7 CFR 457.109 - Sugar Beet Crop Insurance Provisions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 6 2012-01-01 2012-01-01 false Sugar Beet Crop Insurance Provisions. 457.109 Section... CORPORATION, DEPARTMENT OF AGRICULTURE COMMON CROP INSURANCE REGULATIONS § 457.109 Sugar Beet Crop Insurance Provisions. The Sugar Beet Crop Insurance Provisions for the 1998 and succeeding crop years in countries with...

7 CFR 457.109 - Sugar Beet Crop Insurance Provisions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 6 2014-01-01 2014-01-01 false Sugar Beet Crop Insurance Provisions. 457.109 Section... CORPORATION, DEPARTMENT OF AGRICULTURE COMMON CROP INSURANCE REGULATIONS § 457.109 Sugar Beet Crop Insurance Provisions. The Sugar Beet Crop Insurance Provisions for the 1998 and succeeding crop years in countries with...

Sugar Beet, Energy Beet, and Industrial Beet

USDA-ARS?s Scientific Manuscript database

Sugar beet (Beta vulgaris) is a temperate root crop grown primarily as a source of sucrose for human diets. Breeding has focused on sucrose yield, which is simply the product of total root yield times the proportion of sucrose in the harvested roots, minus loss of sucrose in molasses due to impuriti...

21 CFR 172.585 - Sugar beet extract flavor base.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Sugar beet extract flavor base. 172.585 Section... HUMAN CONSUMPTION Flavoring Agents and Related Substances § 172.585 Sugar beet extract flavor base. Sugar beet extract flavor base may be safely used in food in accordance with the provisions of this...

21 CFR 172.585 - Sugar beet extract flavor base.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Sugar beet extract flavor base. 172.585 Section... HUMAN CONSUMPTION Flavoring Agents and Related Substances § 172.585 Sugar beet extract flavor base. Sugar beet extract flavor base may be safely used in food in accordance with the provisions of this...

21 CFR 172.585 - Sugar beet extract flavor base.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Sugar beet extract flavor base. 172.585 Section... HUMAN CONSUMPTION Flavoring Agents and Related Substances § 172.585 Sugar beet extract flavor base. Sugar beet extract flavor base may be safely used in food in accordance with the provisions of this...

Discrimination of genetically modified sugar beets based on terahertz spectroscopy

NASA Astrophysics Data System (ADS)

Chen, Tao; Li, Zhi; Yin, Xianhua; Hu, Fangrong; Hu, Cong

2016-01-01

The objective of this paper was to apply terahertz (THz) spectroscopy combined with chemometrics techniques for discrimination of genetically modified (GM) and non-GM sugar beets. In this paper, the THz spectra of 84 sugar beet samples (36 GM sugar beets and 48 non-GM ones) were obtained by using terahertz time-domain spectroscopy (THz-TDS) system in the frequency range from 0.2 to 1.2 THz. Three chemometrics methods, principal component analysis (PCA), discriminant analysis (DA) and discriminant partial least squares (DPLS), were employed to classify sugar beet samples into two groups: genetically modified organisms (GMOs) and non-GMOs. The DPLS method yielded the best classification result, and the percentages of successful classification for GM and non-GM sugar beets were both 100%. Results of the present study demonstrate the usefulness of THz spectroscopy together with chemometrics methods as a powerful tool to distinguish GM and non-GM sugar beets.

Influence of Beet necrotic yellow vein virus and freezing temperatures on sugar beet roots in storage

USDA-ARS?s Scientific Manuscript database

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) is a yield limiting sugar beet disease that was observed to influence root resistance to freezing in storage. Thus, studies were conducted to gain a better understanding of the influence BNYVV and freezing on sugar beet roots to improve p...

Influence of beet necrotic yellow vein virus and freezing temperatures on sugar beet roots in storage

USDA-ARS?s Scientific Manuscript database

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) is a yield limiting sugar beet disease that was also observed to influence the roots ability to resist freezing in storage. Roots from 5 commercial sugar beet cultivars (1 susceptible and 4 resistant to BNYVV) were produced in fields unde...

Mapping sugar beet pectin acetylation pattern.

PubMed

Ralet, Marie-Christine; Cabrera, Juan Carlos; Bonnin, Estelle; Quéméner, Bernard; Hellìn, Pilar; Thibault, Jean-François

2005-08-01

Homogalacturonan-derived partly methylated and/or acetylated oligogalacturonates were recovered after enzymatic hydrolysis (endo-polygalacturonase+pectin methyl esterase+side-chain degrading enzymes) of sugar beet pectin followed by anion-exchange and size exclusion chromatography. Around 90% of the GalA and 75% of the acetyl groups present in the initial sugar beet pectin were recovered as homogalacturonan-derived oligogalacturonates, the remaining GalA and acetyl belonging to rhamnogalacturonic regions. Around 50% of the acetyl groups present in sugar beet homogalacturonans were recovered as partly methylated and/or acetylated oligogalacturonates of degree of polymerisation 5 whose structures were determined by electrospray ionization ion trap mass spectrometry (ESI-IT-MSn). 2-O-acetyl- and 3-O-acetyl-GalA were detected in roughly similar amounts but 2,3-di-O-acetylation was absent. Methyl-esterified GalA residues occurred mainly upstream 2-O-acetyl GalA. Oligogalacturonates containing GalA residues that are at once methyl- and acetyl-esterified were recovered in very limited amounts. A tentative mapping of the distribution of acetyl and methyl esters within sugar beet homogalacturonans is proposed. Unsubstituted GalA residues are likely to be present in limited amounts (approximately 10% of total GalA residues), due to the fact that methyl and acetyl groups are assumed to be most often not carried by the same residues.

Structural confirmation of oligosaccharides newly isolated from sugar beet molasses.

PubMed

Abe, Tatsuya; Horiuchi, Kenichi; Kikuchi, Hiroto; Aritsuka, Tsutomu; Takata, Yusuke; Fukushi, Eri; Fukushi, Yukiharu; Kawabata, Jun; Ueno, Keiji; Onodera, Shuichi; Shiomi, Norio

2012-08-27

Sugar beet molasses is a viscous by-product of the processing of sugar beets into sugar. The molasses is known to contain sucrose and raffinose, a typical trisaccharide, with a well-established structure. Although sugar beet molasses contains various other oligosaccharides as well, the structures of those oligosaccharides have not been examined in detail. The purpose of this study was isolation and structural confirmation of these other oligosaccharides found in sugar beet molasses. Four oligosaccharides were newly isolated from sugar beet molasses using high-performance liquid chromatography (HPLC) and carbon-Celite column chromatography. Structural confirmation of the saccharides was provided by methylation analysis, matrix-assisted laser desorption/ionaization time of flight mass spectrometry (MALDI-TOF-MS), and nuclear magnetic resonance (NMR) measurements. The following oligosaccharides were identified in sugar beet molasses: β-D-galactopyranosyl-(1- > 6)-β-D-fructofuranosyl-(2 <-> 1)-α-D-glucopyranoside (named β-planteose), α-D-galactopyranosyl-(1- > 1)-β-D-fructofuranosyl-(2 <-> 1)-α-D-glucopyranoside (named1-planteose), α-D-glucopyranosyl-(1- > 6)-α-D-glucopyranosyl-(1 <-> 2)-β-D-fructofuranoside (theanderose), and β-D-glucopyranosyl-(1- > 3)-α-D-glucopyranosyl-(1 <-> 2)-β-D-fructofuranoside (laminaribiofructose). 1-planteose and laminaribiofructose were isolated from natural sources for the first time.

Does information about sugar source influence consumer liking of products made with beet and cane sugars?

PubMed

Urbanus, Brittany L; Schmidt, Shelly J; Lee, Soo-Yeun

2014-11-01

Beet sugar contains an off-aroma, which was hypothesized to generate expectations on the acceptability of a product made with beet sugar. Thus, the objective of this study was to assess the impact of information about the sugar source (beet vs. cane) on the overall liking of an orange-flavored beverage. One hundred panelists evaluated an orange-flavored powdered beverage mix and beverage made with beet and cane sugars using a 5-phase testing protocol involving a tetrad test and hedonic ratings performed under blind and informed conditions. Tetrad test results indicated that there was a significant difference (P < 0.05) between the beverage mix made with beet sugar and cane sugar; however, no difference was found between the beverage made with beet sugar and cane sugar. Hedonic ratings revealed the significance of information conditions on the panelists evaluation of sugar (F = 24.67, P < 0.001); however, no difference in the liking was identified for the beverage mix or beverage. Average hedonic scores were higher under informed condition compared to blind condition for all products, possibly because labels tend to reduce uncertainty about a product. Results from this study are representative of the responses from the general population and suggest that they are not affected by sugar source information in a beverage product. Based on concerns with the use of beet sugar expressed in the popular press, there may be a subgroup of the population that has a preconceived bias about sugar sources due to their prior experiences and knowledge and, thus, would be influenced by labels indicating the sugar source used in a product. © 2014 Institute of Food Technologists®

Beet curly top resistance in USDA-ARS Kimberly sugar beet germplasm lines, 2016

USDA-ARS?s Scientific Manuscript database

Curly top caused by Beet curly top virus is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. In order to identify no...

Root rot in sugar beet piles at harvest

USDA-ARS?s Scientific Manuscript database

Sugar beet root rots are not only a concern because of reduced yields, but can also be associated with losses in storage. Our primary sugar beet root rot disease problem in the Amalgamated production area is Rhizoctonia root rot. However, this rot frequently only penetrates a short distance past t...

Beet curly top virus strains associated with sugar beet in Idaho, Oregon, and a Western U.S. collection

USDA-ARS?s Scientific Manuscript database

Curly top of sugar beet is a serious, yield limiting disease in semi-arid production areas caused by Beet curly top virus (BCTV) and transmitted by the beet leafhopper. One of the primary means of control for BCTV in sugar beet is host resistance but effectiveness of resistance can vary among BCTV ...

Postharvest Rhizopus rot on sugar beet

USDA-ARS?s Scientific Manuscript database

Rhizopus species have been reported as a minor post-harvest rot on sugar beet, particularly under temperatures above 5 deg C. In 2010, Rhizopus was isolated from beets collected from Michigan storage piles in February at a low frequency. However, recent evidence from Michigan has found a high incide...

Effect of Meloidogyne incognita parasitism on yield and sugar content of sugar beet in Georgia

USDA-ARS?s Scientific Manuscript database

Sugar beet (Beta vulgaris) is typically grown as a summer crop for edible sugar production in the north-central and western US, but it could be incorporated as a winter crop into annual cropping systems in the southern US where the sugar would be used for biofuel and plastic production. Sugar beet ...

Experimental sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2016

USDA-ARS?s Scientific Manuscript database

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 31 experimental cultivars were screened by growing them in a sugar beet field infested with...

Experimental sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2015

USDA-ARS?s Scientific Manuscript database

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 32 commercial cultivars were screened by growing them in a sugar beet field infested with B...

Commercial sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2015

USDA-ARS?s Scientific Manuscript database

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 28 commercial cultivars were screened by growing them in a sugar beet field infested with B...

Commercial sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2016

USDA-ARS?s Scientific Manuscript database

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 22 commercial cultivars were screened by growing them in a sugar beet field infested with B...

Review on sugar beet salt stress studies in Iran

NASA Astrophysics Data System (ADS)

Khayamim, S.; Noshad, H.; Jahadakbar, M. R.; Fotuhi, K.

2017-07-01

Increase of saline lands in most regions of the world and Iran, limit of production increase based on land enhancement and also threat of saline water and soils for crop production make related researches and production of salt tolerant variety to be more serious. There have been many researches about salt stress in Sugar Beet Seed Institute of Iran (SBSI) during several years. Accordingly, the new screening methods for stress tolerance to be continued based on these researches. Previous researches in SBSI were reviewed and results concluded to this study which is presented in this article in three categories including: Agronomy, Breeding and Biotechnology. In agronomy researches, suitable planting medium, EC, growth stage and traits for salinity tolerance screening were determined and agronomic technique such as planting date, planting method and suitable nutrition for sugar beet under salt stress were introduced. Sand was salinizied by saline treatments two times more than Perlit so large sized Perlit is suitable medium for saline studies. Sugar beet genotypes screening for salt tolerance and should be conducted at EC=20 in laboratory and EC= 16 dS/M in greenhouse. Although sugar beet seed germination has been known as more susceptible stage to salinity, it seems establishment is more susceptible than germination in which salinity will cause 70-80% decrease in plant establishment. Measurements of leaves Na, K and total carbohydrate at establishment stage would be useful for faster screening of genotypes, based on high and significant correlation of these traits at establishment with yield at harvest time. In breeding section, SBSI genotypes with drought tolerance background would be useful for salinity stress studies and finally there is a need for more research in the field of biotechnology in Iran.

Effect of NaCl on Germination of Sugar Beet

USDA-ARS?s Scientific Manuscript database

Sugar beet is a salt tolerant crop, but is most vulnerable to salinity during germination. The goal of this research is to examine the response to salinity on the germination of sugar beet, ultimately to provide germplasm that has an agronomic use in saline soils around the world. Expanding the char...

First report of sugar beet cyst nematode, Heterodera schachtii, in North Dakota

USDA-ARS?s Scientific Manuscript database

Sugar beet (Beta vulgaris L.) and canola (Brassica napus L.) are major cops in North Dakota with sugar beet production primarily in the eastern part of the state in the Red River Valley and canola production along the northern half of the state from east to west. Both crops are hosts of sugar beet ...

29 CFR 516.18 - Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false Employees employed in certain tobacco, cotton, sugar cane....18 Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are... cigar leaf tobacco, cotton, cottonseed, cotton ginning, sugar cane, sugar processing or sugar beets who...

29 CFR 516.18 - Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Employees employed in certain tobacco, cotton, sugar cane....18 Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are... cigar leaf tobacco, cotton, cottonseed, cotton ginning, sugar cane, sugar processing or sugar beets who...

29 CFR 516.18 - Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false Employees employed in certain tobacco, cotton, sugar cane....18 Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are... cigar leaf tobacco, cotton, cottonseed, cotton ginning, sugar cane, sugar processing or sugar beets who...

29 CFR 516.18 - Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false Employees employed in certain tobacco, cotton, sugar cane....18 Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are... cigar leaf tobacco, cotton, cottonseed, cotton ginning, sugar cane, sugar processing or sugar beets who...

29 CFR 516.18 - Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false Employees employed in certain tobacco, cotton, sugar cane....18 Employees employed in certain tobacco, cotton, sugar cane or sugar beet services, who are... cigar leaf tobacco, cotton, cottonseed, cotton ginning, sugar cane, sugar processing or sugar beets who...

Epigenomics and bolting tolerance in sugar beet genotypes.

PubMed

Hébrard, Claire; Peterson, Daniel G; Willems, Glenda; Delaunay, Alain; Jesson, Béline; Lefèbvre, Marc; Barnes, Steve; Maury, Stéphane

2016-01-01

In sugar beet (Beta vulgaris altissima), bolting tolerance is an essential agronomic trait reflecting the bolting response of genotypes after vernalization. Genes involved in induction of sugar beet bolting have now been identified, and evidence suggests that epigenetic factors are involved in their control. Indeed, the time course and amplitude of DNA methylation variations in the shoot apical meristem have been shown to be critical in inducing sugar beet bolting, and a few functional targets of DNA methylation during vernalization have been identified. However, molecular mechanisms controlling bolting tolerance levels among genotypes are still poorly understood. Here, gene expression and DNA methylation profiles were compared in shoot apical meristems of three bolting-resistant and three bolting-sensitive genotypes after vernalization. Using Cot fractionation followed by 454 sequencing of the isolated low-copy DNA, 6231 contigs were obtained that were used along with public sugar beet DNA sequences to design custom Agilent microarrays for expression (56k) and methylation (244k) analyses. A total of 169 differentially expressed genes and 111 differentially methylated regions were identified between resistant and sensitive vernalized genotypes. Fourteen sequences were both differentially expressed and differentially methylated, with a negative correlation between their methylation and expression levels. Genes involved in cold perception, phytohormone signalling, and flowering induction were over-represented and collectively represent an integrative gene network from environmental perception to bolting induction. Altogether, the data suggest that the genotype-dependent control of DNA methylation and expression of an integrative gene network participate in bolting tolerance in sugar beet, opening up perspectives for crop improvement. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Kimberly sugar beet germplasm evaluated for rhizomania and storage rot resistance in Idaho, 2015

USDA-ARS?s Scientific Manuscript database

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet germplasm lines with resistance to BNYVV and storage rots, 11germplasm lines from the USDA-ARS Kimberly sugar beet program were screened. The lines wer...

40 CFR 409.10 - Applicability; description of the beet sugar processing subcategory.

Code of Federal Regulations, 2013 CFR

2013-07-01

... sugar processing subcategory. 409.10 Section 409.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing Subcategory § 409.10 Applicability; description of the beet sugar processing subcategory. The...

40 CFR 409.10 - Applicability; description of the beet sugar processing subcategory.

Code of Federal Regulations, 2012 CFR

2012-07-01

... sugar processing subcategory. 409.10 Section 409.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing Subcategory § 409.10 Applicability; description of the beet sugar processing subcategory. The...

40 CFR 409.10 - Applicability; description of the beet sugar processing subcategory.

Code of Federal Regulations, 2014 CFR

2014-07-01

... sugar processing subcategory. 409.10 Section 409.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing Subcategory § 409.10 Applicability; description of the beet sugar processing subcategory. The...

40 CFR 409.10 - Applicability; description of the beet sugar processing subcategory.

Code of Federal Regulations, 2010 CFR

2010-07-01

... sugar processing subcategory. 409.10 Section 409.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing Subcategory § 409.10 Applicability; description of the beet sugar processing subcategory. The...

40 CFR 409.10 - Applicability; description of the beet sugar processing subcategory.

Code of Federal Regulations, 2011 CFR

2011-07-01

... sugar processing subcategory. 409.10 Section 409.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing Subcategory § 409.10 Applicability; description of the beet sugar processing subcategory. The...

Biogas from sugar beet press pulp as substitute of fossil fuel in sugar beet factories.

PubMed

Brooks, L; Parravicini, V; Svardal, K; Kroiss, H; Prendl, L

2008-01-01

Sugar beet press pulp (SBP) accumulates as a by-product in sugar factories and it is generally silaged or dried to be used as animal food. Rising energy prices and the opening of the European Union sugar market has put pressure on the manufacturers to find alternatives for energy supply. The aim of this project was to develop a technology in the treatment of SBP that would lead to savings in energy consumption and would provide a more competitive sugar production from sugar beets. These goals were met by the anaerobic digestion of SBP for biogas production. Lab-scale experiments confirmed the suitability of SBP as substrate for anaerobic bacteria. Pilot-scale experiments focused on process optimization and procedures for a quick start up and operational control. Both single-stage and two-stage process configurations showed similar removal efficiency. A stable biogas production could be achieved in single-stage at a maximum volumetric loading rate of 10 kgCSB/(m(3) x d). Degradation efficiency was 75% for VS and 72% for COD. Average specific gas production reached 530 NL/kgCOD(SBP) or 610 NL/kgVS(SBP). (CH(4): 50 to 53%). The first large-scale biogas plant was put into operation during the sugar processing period 2007 at a Hungarian sugar factory. Digesting approximately 50% of the SBP (800 t/d, 22%TS), the biogas produced could substitute about 40% of the natural gas required for the thermal energy supply within the sugar processing. Copyright IWA Publishing 2008.

Beet curly top virus strains associated with sugar beet in Idaho, Oregon, and a survey collection

USDA-ARS?s Scientific Manuscript database

Curly top of sugar beet is a serious yield limiting disease in semi-arid production areas caused by Beet curly top virus (BCTV) and vectored by the beet leafhopper (Circulifer tennellus). The primary means of control for BCTV is host resistance, but effectiveness of resistance can vary among BCTV s...

76 FR 62339 - Domestic Sugar Program-2011-Crop Cane Sugar and Beet Sugar Marketing Allotments and Company...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-07

... Sugar and Beet Sugar Marketing Allotments and Company Allocations AGENCY: Commodity Credit Corporation... the fiscal year (FY) 2012 State sugar marketing allotments and company allocations to sugarcane and... required to publish the determinations establishing, adjusting, or suspending sugar marketing allotments in...

Genomic selection in sugar beet breeding populations

PubMed Central

2013-01-01

Background Genomic selection exploits dense genome-wide marker data to predict breeding values. In this study we used a large sugar beet population of 924 lines representing different germplasm types present in breeding populations: unselected segregating families and diverse lines from more advanced stages of selection. All lines have been intensively phenotyped in multi-location field trials for six agronomically important traits and genotyped with 677 SNP markers. Results We used ridge regression best linear unbiased prediction in combination with fivefold cross-validation and obtained high prediction accuracies for all except one trait. In addition, we investigated whether a calibration developed based on a training population composed of diverse lines is suited to predict the phenotypic performance within families. Our results show that the prediction accuracy is lower than that obtained within the diverse set of lines, but comparable to that obtained by cross-validation within the respective families. Conclusions The results presented in this study suggest that a training population derived from intensively phenotyped and genotyped diverse lines from a breeding program does hold potential to build up robust calibration models for genomic selection. Taken together, our results indicate that genomic selection is a valuable tool and can thus complement the genomics toolbox in sugar beet breeding. PMID:24047500

Beta vulgaris L. serine proteinase inhibitor gene expression correlates to insect pest resistance in sugar beet

USDA-ARS?s Scientific Manuscript database

Analyzing genes that can be used for improving sugar beet resistance to the sugar beet root maggot (SBRM, Tetanops myopaeformis Roder), one of the most destructive insect pests of sugar beet in North America, was a major goal in our investigation. We report on the expression patterns of a sugar beet...

Yield Potential of Sugar Beet – Have We Hit the Ceiling?

PubMed Central

Hoffmann, Christa M.; Kenter, Christine

2018-01-01

The yield of sugar beet has continuously increased in the past decades. The question arises, whether this progress will continue in the future. A key factor for increasing yield potential of the crop is breeding progress. It was related to a shift in assimilate partitioning in the plant toward more storage carbohydrates (sucrose), whereas structural carbohydrates (leaves, cell wall compounds) unintendedly declined. The yield potential of sugar beet was estimated at 24 t sugar ha-1. For maximum yield, sufficient growth factors have to be available and the crop has to be able to fully utilize them. In sugar beet, limitations result from the lacking coincidence of maximum irradiation rates and full canopy cover, sink strength for carbon assimilation and high water demand, which cannot be met by rainfall alone. After harvest, sugar losses during storage occur. The paper discusses options for a further increase in yield potential, like autumn sowing of sugar beet, increasing sink strength and related constraints. It is prospected that yield increase by further widening the ratio of storage and structural carbohydrates will come to its natural limit as a certain cell wall stability is necessary. New challenges caused by climate change and by prolonged processing campaigns will occur. Thus breeding for improved pathogen resistance and storage properties will be even more important for successful sugar beet production than a further increase in yield potential itself. PMID:29599787

Estrogenicity of sugar beet by-products used as animal feeds

USDA-ARS?s Scientific Manuscript database

A veterinarian observed a reduction in embryo transfer success rates on beef and dairy farms in Minnesota, which were both feeding sugar beet by-products. Beet tailings and pelleted post-extraction beet pulp, associated with the affected farms were analyzed for estrogenicity by E-Screen (proliferati...

A nine-scaffold genome assembly of the nine chromosome sugar beet

USDA-ARS?s Scientific Manuscript database

A sugar beet genome sequence is required to take full advantage of the increasingly powerful approaches directed a single nucleotide resolution across the whole genome. A high quality reference genome serves as a benchmark from which other genotypes might be compared and exploited for sugar beet imp...

Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production

PubMed Central

Berłowska, Joanna; Balcerek, Maria; Dziekońska-Kubczak, Urszula; Patelski, Piotr; Dziugan, Piotr

2016-01-01

Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) and Pichia stipitis (0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching 26.9 ± 1.2 g/L and 86.5 ± 2.1% fermentation efficiency relative to the theoretical yield. PMID:27722169

Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production.

PubMed

Berłowska, Joanna; Pielech-Przybylska, Katarzyna; Balcerek, Maria; Dziekońska-Kubczak, Urszula; Patelski, Piotr; Dziugan, Piotr; Kręgiel, Dorota

2016-01-01

Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015-0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red ( S. cerevisiae ) (1 g/L) and Pichia stipitis (0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching 26.9 ± 1.2 g/L and 86.5 ± 2.1% fermentation efficiency relative to the theoretical yield.

Closing the Yield Gap of Sugar Beet in the Netherlands-A Joint Effort.

PubMed

Hanse, Bram; Tijink, Frans G J; Maassen, Jurgen; van Swaaij, Noud

2018-01-01

The reform of the European Union's sugar regime caused potential decreasing beet prices. Therefore, the Speeding Up Sugar Yield (SUSY) project was initiated. At the start, a 3 × 15 target was formulated: in 2015 the national average sugar yield in the Netherlands equals 15 t/ha (60% of the sugar beet potential) and the total variable costs 15 euro/t sugar beet, aspiring a saving on total variable costs and a strong increase in sugar yield. Based on their average sugar yield in 2000-2004, 26 pairs of "type top" (high yielding) and "type average" (average yielding) growers were selected from all sugar beet growing regions in the Netherlands. On the fields of those farmers, all measures of sugar beet cultivation were investigated, including cost calculation and recording phytopathological, agronomical and soil characteristics in 2006 and 2007. Although there was no significant difference in total variable costs, the "type top" growers yielded significantly 20% more sugar in each year compared to the "type average" growers. Therefore, the most profitable strategy for the growers is maximizing sugar yield and optimizing costs. The difference in sugar yield between growers could be explained by pests and diseases (50%), weed control (30%), soil structure (25%) and sowing date (14%), all interacting with each other. The SUSY-project revealed the effect of the grower's management on sugar yield. As a follow up for the SUSY-project, a growers' guide "Suikerbietsignalen" was published, Best Practice study groups of growers were formed and trainings and workshops were given and field days organized. Further, the benchmarking and feedback on the crop management recordings and the extension on variety choice, sowing performance, foliar fungi control and harvest losses were intensified. On the research part, a resistance breaking strain of the Beet Necrotic Yellow Vein Virus (BNYVV) and a new foliar fungus, Stemphylium beticola , were identified and options for control were

Successful application of dextranase in sugar beet factories

USDA-ARS?s Scientific Manuscript database

Dextranases are sometimes applied to hydrolyze dextran polysaccharide in sugar manufacture when bacterial deterioration of sugar beet has occurred. Unfortunately, dextranases only have a small market and low volume sales compared to many other industrial enzymes. Consequently, research and develop...

Ft. Collins Sugar Beet Germplasm Evaluated for Resistance to Rhizomania and Storability in Idaho, 2010

USDA-ARS?s Scientific Manuscript database

Sugar beet germplasm and commercial check cultivars were evaluated in a sprinkler-irrigated sugar beet field near Kimberly, ID where sugar beet was grown in 2009. The field trial relied on natural inoculum for rhizomania development. The seed was treated with clothianidin (2.1 oz a.i. per 100,000 ...

Evaluation of fungicide and biological treatments for control of fungal storage rots in sugar beet, 2014

USDA-ARS?s Scientific Manuscript database

Preventing sucrose losses in storage is important to the economic viability of the sugar beet industry. In an effort to establish additional measures for reducing sucrose losses in storage, ten fungicide and/or biological treatments were evaluated on sugar beet roots in a commercial sugar beet stor...

Economic feasibility of the sugar beet-to-ethylene value chain.

PubMed

Althoff, Jeroen; Biesheuvel, Kees; De Kok, Ad; Pelt, Henk; Ruitenbeek, Matthijs; Spork, Ger; Tange, Jan; Wevers, Ronald

2013-09-01

As part of a long-term strategy toward renewable feedstock, a feasibility study into options for the production of bioethylene by integrating the sugar beet-to-ethanol-to-ethylene value chain. Seven business cases were studied and tested for actual economic feasibility of alternative sugar-to-ethanol-to-ethylene routes in comparison to fossil-fuel alternatives. An elaborate model was developed to assess the relevant operational and financial aspects of each business case. The calculations indicate that bioethylene from sugar beet is not commercially viable under current market conditions. In light of expected global energy and feedstock prices it is also reasonable to expect that this will not change in the near future. To consider biorenewable sources as starting material, they need to be low in cost (compared to sugar beets) and also require less capital and energy-intensive methods for the conversion to chemicals. In general, European sugar prices will be too high for many chemical applications. Future efforts for in sugar-to-chemicals routes should, therefore, focus on integrated process routes and process intensification and/or on products that contain a significant part of the original carbohydrate backbone. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citramalic acid and salicylic acid in sugar beet root exudates solubilize soil phosphorus

PubMed Central

2011-01-01

Background In soils with a low phosphorus (P) supply, sugar beet is known to intake more P than other species such as maize, wheat, or groundnut. We hypothesized that organic compounds exuded by sugar beet roots solubilize soil P and that this exudation is stimulated by P starvation. Results Root exudates were collected from plants grown in hydroponics under low- and high-P availability. Exudate components were separated by HPLC, ionized by electrospray, and detected by mass spectrometry in the range of mass-to-charge ratio (m/z) from 100 to 1000. Eight mass spectrometric signals were enhanced at least 5-fold by low P availability at all harvest times. Among these signals, negative ions with an m/z of 137 and 147 were shown to originate from salicylic acid and citramalic acid. The ability of both compounds to mobilize soil P was demonstrated by incubation of pure substances with Oxisol soil fertilized with calcium phosphate. Conclusions Root exudates of sugar beet contain salicylic acid and citramalic acid, the latter of which has rarely been detected in plants so far. Both metabolites solubilize soil P and their exudation by roots is stimulated by P deficiency. These results provide the first assignment of a biological function to citramalic acid of plant origin. PMID:21871058

Response of sugar beet recombinant inbred lines to post-harvest rot fungi

USDA-ARS?s Scientific Manuscript database

Sugar beet is commonly stored in outdoor piles prior to processing. During this storage period the crop is subject to multiple post-harvest rots. Resistance to three post harvest rots was identified in two sugar beet germplasm in the 1970s, but there has been little work done on host resistance to p...

Ft. Collins sugar beet germplasm evaluated for rhizomania and storage rot resistance in Idaho, 2015

USDA-ARS?s Scientific Manuscript database

Fifty-seven sugar beet (Beta vulgaris L.) lines from the USDA-ARS Ft. Collins sugar beet program and four check cultivars were screened for resistance to Beet necrotic yellow vein virus (BNYVV), the causal agent of rhizomania, and storage rot. The rhizomania evaluation was conducted at the USDA-ARS...

First report of DMI insensitive Cercospora beticola on sugar beet in Ontario, Canada

USDA-ARS?s Scientific Manuscript database

Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is an economically important foliar disease of sugar beet in Ontario, Canada and worldwide. Fungicides are an important tool in the control of CLS. The first demethylation inhibitor (DMI) fungicide for sugar beet was regi...

TREATMENT OF BEET SUGAR PLANT SEWAGE

PubMed Central

Pearse, Langdon; Greeley, Samuel A.

1920-01-01

Beet sugar is an industry yearly attaining greater and greater importance. Likewise the disposal of the wastes is a problem of increasing consequence in various sections of the country. This paper and the discussions constitute an unusual assembling of the facts, valuable to local authorities and those commercially interested, alike. PMID:18010285

A novel penicillium sp. causes rot in stored sugar beet roots in Idaho

USDA-ARS?s Scientific Manuscript database

Penicillium vulpinum along with a number of other fungi can lead to the rot of stored sugar beet roots. However, Penicillium isolates associated with necrotic lesions on roots from a recent sugar beet storage study were determined to be different from P. vulpinum and other recognized Penicillium sp...

First report of QoI resistance in Alternaria spp. infecting sugar beet (Beta vulgaris) in Michigan, USA

USDA-ARS?s Scientific Manuscript database

Alternaria leaf spot (ALS) of sugar beet is caused by Alternaria spp. in the A. alternata species complex. ALS is common wherever sugar beet is grown, but historically has been a minor issue for sugar beet production in the USA with damage usually not affecting crop yield significantly. Occurrence o...

Management of curly top in sugar beet with seed and foliar insecticides

USDA-ARS?s Scientific Manuscript database

Curly top in sugar beet can result in severe yield losses and is caused by Beet severe curly top virus (BSCTV) and other closely related Curtovirus spp. which are vectored by the beet leafhopper. Neonicotinoid seed treatments (Cruiser, NipsIt, and Poncho) have been shown to be an effective suppleme...

Co-Digestion of Sugar Beet Silage Increases Biogas Yield from Fibrous Substrates

PubMed Central

Einfalt, Daniel; Kazda, Marian

2016-01-01

This study tested the hypothesis that the easily degradable carbohydrates of the sugar beet silage (S) will improve the anaerobic digestion of grass silage (G) more profoundly compared to co-digestion of sugar beet silage with maize silage (M). M : S and G : S mixtures were tested in two continuous laboratory-scale AD experiments at volatile solid ratios of 1 : 0, 6 : 1, 3 : 1, and 1 : 3 at organic loading rates of 1.5 kgVS m−3 day−1. While the sugar beet effects in mixtures with maize silage were negligible, co-digestion with grass silage showed a beneficial performance. There, the specific methane production rate was 0.27 lN kg−1VS h−1at G : S ratio of 6 : 1 compared to G : S 1 : 0 with 0.14 lN kg−1VS h−1. In comparison to G : S 1 : 0, about 44% and 62% higher biogas yields were obtained at G : S 6 : 1 and 3 : 1, respectively. Also, the highest methane concentration was found in G : S at ratio of 1 : 3. Synergistic increase of methane yield was found in co-digestion in both experiments, but higher effect was realized in G : S, independently of the amount of sugar beet silage. The findings of this study emphasize the improvement of AD of grass silage by even low addition of sugar beet silage. PMID:27807538

Co-Digestion of Sugar Beet Silage Increases Biogas Yield from Fibrous Substrates.

PubMed

Ahmed, Sharif; Einfalt, Daniel; Kazda, Marian

2016-01-01

This study tested the hypothesis that the easily degradable carbohydrates of the sugar beet silage (S) will improve the anaerobic digestion of grass silage (G) more profoundly compared to co-digestion of sugar beet silage with maize silage (M). M : S and G : S mixtures were tested in two continuous laboratory-scale AD experiments at volatile solid ratios of 1 : 0, 6 : 1, 3 : 1, and 1 : 3 at organic loading rates of 1.5 kgVS m -3  day -1 . While the sugar beet effects in mixtures with maize silage were negligible, co-digestion with grass silage showed a beneficial performance. There, the specific methane production rate was 0.27 l N  kg -1 VS h -1 at G : S ratio of 6 : 1 compared to G : S 1 : 0 with 0.14 l N  kg -1 VS h -1 . In comparison to G : S 1 : 0, about 44% and 62% higher biogas yields were obtained at G : S 6 : 1 and 3 : 1, respectively. Also, the highest methane concentration was found in G : S at ratio of 1 : 3. Synergistic increase of methane yield was found in co-digestion in both experiments, but higher effect was realized in G : S, independently of the amount of sugar beet silage. The findings of this study emphasize the improvement of AD of grass silage by even low addition of sugar beet silage.

Foliar insecticides for the control of curly top in Idaho sugar beet, 2017

USDA-ARS?s Scientific Manuscript database

Curly top caused by Beet curly top virus (BCTV) is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. The neonicotiono...

A nine-scaffold genome assembly of the nine chromosome sugar beet

USDA-ARS?s Scientific Manuscript database

Over the course of 20 months, we assembled a sugar beet genome (700 - 800 Mb) into a close representation of the nine haploid chromosomes of beet. This result was obtained by sequentially assembling sequences >40 kb in length, orienting these assemblies via optical mapping, and scaffolding with in v...

High resolution melting (HRM) analysis in sugar beet: identification of SNP markers associated to Fusarium resistance

USDA-ARS?s Scientific Manuscript database

Fusarium spp. cause severe damage in many agricultural crops including sugar beet. Sugar beet needs to be protected from these soil borne pathogens to guarantee an optimal sugar yield in the field. The genetic control is the key to overcoming this disease. Identification of single nucleotide polymor...

Response of sugar beet (Beta vulgaris) recombinant inbred lines to post-harvest rot fungi

USDA-ARS?s Scientific Manuscript database

Sugar beet (Beta vulgaris) is commonly stored in outdoor piles prior to processing for food and animal feed. During this storage period the crop is subject to multiple post-harvest rots. Resistance to three post harvest rots was identified in two sugar beet germplasm in the 1970s, but there has been...

Insect resistance to sugar beet pests mediated by a Beta vulgaris proteinase inhibitor transgene

USDA-ARS?s Scientific Manuscript database

We transformed sugar beet (Beta vulgaris) hairy roots and Nicotiana benthamiana plants with a Beta vulgaris root gene (BvSTI) that codes for a serine proteinase inhibitor. BvSTI is a root gene cloned from the F1016 breeding line that has moderate levels of resistance to the sugar beet root maggot ...

Investigation of Copper Sorption by Sugar Beet Processing Lime Waste

EPA Science Inventory

In the western United States, sugar beet processing for sugar recovery generates a lime-based waste product (~250,000 Mg yr^-1) that has little liming value in the region’s calcareous soils. This area has recently experienced an increase in dairy production, with dairi...

USE OF GREEN MANURE CROPS AND SUGAR BEET VARIETIES TO CONTROL HETERODERA BETAE.

PubMed

Raaijmakers, E

2014-01-01

Although it is less studied than the white beet cyst nematode (Heterodera schachtii), the yellow beet cyst nematode (H. betae) has been found in many countries in Europe. For example in The Netherlands, France and Spain. H. betae causes yield losses on sandy soils. A high infestation can result in loss of complete plants. In The Netherlands, this nematode is especially found in the south eastern and north eastern part, where it occurs on 18% and 5% of the fields, respectively. From a project of the Dutch Sugar beet Research Institute IRS (SUSY) on factors explaining differences in sugar yield, this nematode was one of the most important factors reducing sugar yields on sandy soils. Until 2008, the only way to control H. betae was by reducing the number of host crops in the crop rotation. Host crops are crops belonging to the families of Cruciferae, Chenopodiaceae, Polygonaceae, Caryophyllaceae and Leguminosea. In order to find more control measures, research was done to investigate the host status of different green manure crops and the resistance and tolerance of different sugar beet varieties to H. betae. White mustard (Sinapis alba) and oil seed radish (Raphanus sativus spp. oleiferus) varieties resistant to H. schachtii were investigated for their resistance against H. betae. A climate room trial and a field trial with white mustard and oil seed radish were conducted in 2007 and 2008, respectively. Results show that H. betae could multiply on susceptible white mustard and susceptible oil seed radish, but not on the H. schachtii resistant varieties. In climate room trials in 2009, 2010 and 2011 and field trials in 2010, 2011 and 2012, the effect of different sugar beet varieties on the multiplication of H. betae and the effect of H. betae on yield at different infestation levels was investigated. Sugar beet varieties with resistance genes to H. schachtii (from Beta procumbens or B. maritima) were selected. Varieties with resistance genes from these sources were

Sexual crossing of thermophilic fungus Myceliophthora heterothallica improved enzymatic degradation of sugar beet pulp.

PubMed

Aguilar-Pontes, Maria Victoria; Zhou, Miaomiao; van der Horst, Sjors; Theelen, Bart; de Vries, Ronald P; van den Brink, Joost

2016-01-01

Enzymatic degradation of plant biomass requires a complex mixture of many different enzymes. Like most fungi, thermophilic Myceliophthora species therefore have a large set of enzymes targeting different linkages in plant polysaccharides. The majority of these enzymes have not been functionally characterized, and their role in plant biomass degradation is unknown. The biotechnological challenge is to select the right set of enzymes to efficiently degrade a particular biomass. This study describes a strategy using sexual crossing and screening with the thermophilic fungus Myceliophthora heterothallica to identify specific enzymes associated with improved sugar beet pulp saccharification. Two genetically diverse M. heterothallica strains CBS 203.75 and CBS 663.74 were used to generate progenies with improved growth on sugar beet pulp. One progeny, named SBP.F1.2.11, had a different genetic pattern from the parental strains and had improved saccharification activity after the growth on 3 % sugar beet pulp. The improved SBP saccharification was not explained by altered activities of the major (hemi-)cellulases. Exo-proteome analysis of progeny and parental strains after 7-day growth on sugar beet pulp showed that only 17 of the 133 secreted CAZy enzymes were more abundant in progeny SBP.F1.2.11. Particularly one enzyme belonging to the carbohydrate esterase family 5 (CE5) was more abundant in SBP.F1.2.11. This CE5-CBM1 enzyme, named as Axe1, was phylogenetically related to acetyl xylan esterases. Biochemical characterization of Axe1 confirmed de-acetylation activity with optimal activities at 75-85 °C and pH 5.5-6.0. Supplementing Axe1 to CBS 203.75 enzyme set improved release of xylose and glucose from sugar beet pulp. This study identified beneficial enzymes for sugar beet pulp saccharification by selecting progeny with improved growth on this particular substrate. Saccharification of sugar beet pulp was improved by supplementing enzyme mixtures with a previously

Steam explosion and fermentation of sugar beets from Southern Florida and the Midwestern United States

USDA-ARS?s Scientific Manuscript database

Sugar beets have recently gained interest for cultivation in southern Florida for their economic potential as cattle feed, a feedstock for ethanol production and their use to improve the quality of water via soil nutrient accumulation. Sugar beets grown in southern Florida, Minnesota and Nebraska we...

Properties of Two Novel Esterases Identified from Culture Supernatant of Penicillium purpurogenum Grown on Sugar Beet Pulp

PubMed Central

Oleas, Gabriela; Callegari, Eduardo; Sepulveda, Romina; Eyzaguirre, Jaime

2017-01-01

Background The filamentous fungus Penicillium purpurogenum grows on a variety of natural carbon sources, such as sugar beet pulp, and secretes to the medium a large number of enzymes that degrade the carbohydrate components of lignocellulose. Sugar beet pulp is rich in pectin, and the purpose of this work is to identify novel esterases produced by the fungus, which may participate in pectin degradation. Methods and findings Partially purified culture supernatants of the fungus grown on sugar beet pulp were subjected to mass spectrometry analysis. Peptides thus identified, which may be part of potential esterases were probed against the proteins deduced from the fungal genome sequence. The cDNAs of two putative esterases identified were expressed in Pichia pastoris and their properties studied. One of these enzymes, named FAET, is a feruloyl esterase, while the other, PE, is classified as a pectin methyl esterase. Conclusions These findings add to our knowledge of the enzymology of pectin degradation by Penicillium purpurogenum, and define properties of two novel esterases acting on de-esterification of pectin. Their availability may be useful as tools for the study of pectin structure and degradation. PMID:28828411

Disease detection in sugar beet fields: a multi-temporal and multi-sensoral approach on different scales

NASA Astrophysics Data System (ADS)

Mahlein, Anne-Katrin; Hillnhütter, Christian; Mewes, Thorsten; Scholz, Christine; Steiner, Ulrike; Dehne, Heinz-Willhelm; Oerke, Erich-Christian

2009-09-01

Depending on environmental factors fungal diseases of crops are often distributed heterogeneously in fields. Precision agriculture in plant protection implies a targeted fungicide application adjusted these field heterogeneities. Therefore an understanding of the spatial and temporal occurrence of pathogens is elementary. As shown in previous studies, remote sensing techniques can be used to detect and observe spectral anomalies in the field. In 2008, a sugar beet field site was observed at different growth stages of the crop using different remote sensing techniques. The experimental field site consisted of two treatments. One plot was sprayed with a fungicide to avoid fungal infections. In order to obtain sugar beet plants infected with foliar diseases the other plot was not sprayed. Remote sensing data were acquired from the high-resolution airborne hyperspectral imaging ROSIS in July 2008 at sugar beet growth stage 39 and from the HyMap sensor systems in August 2008 at sugar beet growth stage 45, respectively. Additionally hyperspectral signatures of diseased and non-diseased sugar beet plants were measured with a non-imaging hand held spectroradiometer at growth stage 49 in September. Ground truth data, in particular disease severity were collected at 50 sampling points in the field. Changes of reflection rates were related to disease severity increasing with time. Erysiphe betae causing powdery mildew was the most frequent leaf pathogen. A classification of healthy and diseased sugar beets in the field was possible by using hyperspectral vegetation indices calculated from canopy reflectance.

Technical and economic assessments of storage techniques for long-term retention of industrial-beet sugar for non-food industrial fermentations

NASA Astrophysics Data System (ADS)

Vargas-Ramirez, Juan Manuel

Industrial beets may compete against corn grain as an important source of sugars for non-food industrial fermentations. However, dependable and energy-efficient systems for beet sugar storage and processing are necessary to help establish industrial beets as a viable sugar feedstock. Therefore, technical and economic aspects of beet sugar storage and processing were evaluated. First, sugar retention was evaluated in whole beets treated externally with either one of two antimicrobials or a senescence inhibitor and stored for 36 wk at different temperature and atmosphere combinations. Although surface treatment did not improve sugar retention, full retention was enabled by beet dehydration caused by ambient air at 25 °C and with a relative humidity of 37%. This insight led to the evaluation of sugar retention in ground-beet tissue ensiled for 8 wk at different combinations of acidic pH, moisture content (MC), and sugar:solids. Some combinations of pH ≤ 4.0 and MC ≤ 67.5% enabled retentions of at least 90%. Yeast fermentability was also evaluated in non-purified beet juice acidified to enable long-term storage and partially neutralized before fermentation. None of the salts synthesized through juice acidification and partial neutralization inhibited yeast fermentation at the levels evaluated in that work. Conversely, yeast fermentation rates significantly improved in the presence of ammonium salts, which appeared to compensate for nitrogen deficiencies. Capital and operating costs for production and storage of concentrated beet juice for an ethanol plant with a production capacity of 76 x 106 L y-1 were estimated on a dry-sugar basis as U.S. ¢34.0 kg-1 and ¢2.2 kg-1, respectively. Storage and processing techniques evaluated thus far prove that industrial beets are a technically-feasible sugar feedstock for ethanol production.

Temperature, Moisture, and Fungicide Effects in Managing Rhizoctonia Root and Crown Rot of Sugar Beet

USDA-ARS?s Scientific Manuscript database

Rhizoctonia solani AG-2-2 is the causal agent of Rhizoctonia root and crown rot in sugar beet. To assess the capacity at which other anastomosis groups (AGs) are able to infect sugar beet, 15 AGs and subgroups were tested for pathogenicity on resistant (FC708 CMS) and susceptible (Monohikari) seedl...

Cultivar Selection for Sugar Beet Root Rot Resistance

USDA-ARS?s Scientific Manuscript database

Fungal and bacterial root rots in sugar beet caused by Rhizoctonia solani (Rs) and Leuconostoc mesenteroides subsp. dextranicum (Lm) can lead to root yield losses greater than 50%. To reduce the impact of these root rots on sucrose loss in the field, storage, and factories, studies were conducted t...

Yield potential of spring-harvested sugar beet depends on autumn planting time

USDA-ARS?s Scientific Manuscript database

Sugar crops grown for biofuel production provide a source of simple sugars that can readily be made into advanced biofuels. In the mild climate of the southeastern USA, sugar beet can be grown as a winter crop, providing growers with an alternative crop. Experiments evaluated autumn planting dates...

Preparation and properties of water and glycerol-plasticized sugar beet pulp plastics

USDA-ARS?s Scientific Manuscript database

Sugar beet pulp (SBP), the residue from sugar extraction, was compounded and turned into thermoplastic composite materials. The compounding was performed using a common twin screw compounding extruder and water and glycerol were used as plasticizers. The plasticization of SBP utilized the water-solu...

Genomic selection in sugar beet breeding populations.

PubMed

Würschum, Tobias; Reif, Jochen C; Kraft, Thomas; Janssen, Geert; Zhao, Yusheng

2013-09-18

Genomic selection exploits dense genome-wide marker data to predict breeding values. In this study we used a large sugar beet population of 924 lines representing different germplasm types present in breeding populations: unselected segregating families and diverse lines from more advanced stages of selection. All lines have been intensively phenotyped in multi-location field trials for six agronomically important traits and genotyped with 677 SNP markers. We used ridge regression best linear unbiased prediction in combination with fivefold cross-validation and obtained high prediction accuracies for all except one trait. In addition, we investigated whether a calibration developed based on a training population composed of diverse lines is suited to predict the phenotypic performance within families. Our results show that the prediction accuracy is lower than that obtained within the diverse set of lines, but comparable to that obtained by cross-validation within the respective families. The results presented in this study suggest that a training population derived from intensively phenotyped and genotyped diverse lines from a breeding program does hold potential to build up robust calibration models for genomic selection. Taken together, our results indicate that genomic selection is a valuable tool and can thus complement the genomics toolbox in sugar beet breeding.

Cultivar selection for bacterial root rot in sugar beet

USDA-ARS?s Scientific Manuscript database

Bacterial root rot of sugar beet caused by Leuconostoc mesenteroides subsp. dextranicum is a disease problem recently described in the United States, which has frequently been found in association with Rhizoctonia root rot. To reduce the impact of bacterial root rot on sucrose loss in the field, st...

Descriptive parameters for revealing substitution patterns of sugar beet pectins using pectolytic enzymes.

PubMed

Remoroza, C; Buchholt, H C; Gruppen, H; Schols, H A

2014-01-30

Enzymatic fingerprinting was applied to sugar beet pectins (SBPs) modified by either plant or fungal pectin methyl esterases and alkali catalyzed de-esterification to reveal the ester distributions over the pectin backbone. A simultaneous pectin lyase (PL) treatment to the commonly used endo-polygalacturonase (endo-PG) degradation showed to be effective in degrading both high and low methylesterified and/or acetylated homogalaturonan regions of SBP simultaneously. Using LC-HILIC-MS/ELSD, we studied in detail all the diagnostic oligomers present, enabling us to discriminate between differently prepared sugar beet pectins having various levels of methylesterification and acetylation. Furthermore, distinction between commercially extracted and de-esterified sugar beet pectin having different patterns of substitution was achieved by using novel descriptive pectin parameters. In addition to DBabs approach for nonmethylesterified sequences degradable by endo-PG, the "degree of hydrolysis" (DHPG) representing all partially saturated methylesterified and/or acetylated galacturonic acid (GalA) moieties was introduced as a new parameter. Consequently, the description DHPL has been introduced to quantify all esterified unsaturated GalA oligomers. Copyright © 2013 Elsevier Ltd. All rights reserved.

Illumina-based analysis of endophytic bacterial diversity and space-time dynamics in sugar beet on the north slope of Tianshan mountain.

PubMed

Shi, YingWu; Yang, Hongmei; Zhang, Tao; Sun, Jian; Lou, Kai

2014-01-01

Plants harbors complex and variable microbial communities. Endophytic bacteria play an important function and potential role more effectively in developing sustainable systems of crop production. To examine how endophytic bacteria in sugar beet (Beta vulgaris L.) vary across both host growth period and location, PCR-based Illumina was applied to revealed the diversity and stability of endophytic bacteria in sugar beet on the north slope of Tianshan mountain, China. A total of 60.84 M effective sequences of 16S rRNA gene V3 region were obtained from sugar beet samples. These sequences revealed huge amount of operational taxonomic units (OTUs) in sugar beet, that is, 19-121 OTUs in a beet sample, at 3 % cutoff level and sequencing depth of 30,000 sequences. We identified 13 classes from the resulting 449,585 sequences. Alphaproteobacteria were the dominant class in all sugar beets, followed by Acidobacteria, Gemmatimonadetes and Actinobacteria. A marked difference in the diversity of endophytic bacteria in sugar beet for different growth periods was evident. The greatest number of OTUs was detected during rossette formation (109 OTUs) and tuber growth (146 OTUs). Endophytic bacteria diversity was reduced during seedling growth (66 OTUs) and sucrose accumulation (95 OTUs). Forty-three OTUs were common to all four periods. There were more tags of Alphaproteobacteria and Gammaproteobacteria in Shihezi than in Changji. The dynamics of endophytic bacteria communities were influenced by plant genotype and plant growth stage. To the best of our knowledge, this study is the first application of PCR-based Illumina pyrosequencing to characterize and compare multiple sugar beet samples.

Effect of sugar beet tubers as a partial replacer to green fodder on production performance and economics of lactating Surti buffaloes in lean period.

PubMed

Sorathiya, L M; Patel, M D; Tyagi, K K; Fulsoundar, A B; Raval, A P

2015-01-01

The objective of this study was to evaluate the effects of sugar beet tubers as a replacer to green fodder on production performance and economics of lactating Surti buffaloes. This trial was conducted at the Livestock Research Station, Navsari Agricultural University, Navsari. Twenty lactating Surti buffaloes in a changeover experimental design were selected to assess the effects of replacing green fodder with sugar beet (Beta vulgaris L.) tubers on production performance, economics of feeding sugar beet and blood biochemical profile. Half (50%) of the hybrid Napier was replaced with sliced sugar beet tubers in the ration of experimental animals. Partial replacement of hybrid Napier with that of sugar beet tubers numerically improved dry matter intake, milk yield, 4% fat corrected milk and milk composition parameters such as fat, solid non-fat, protein and lactose, but not significantly. The blood parameters were in normal range and non-significant except that of glucose and triglycerides, which were increased in the sugar beet group. Replacing sugar beet tubers also proved to be cost-effective with improved net profit around Rs. 6.63/day. It can be concluded that 50% hybrid Napier fodder can be replaced with sugar beet tubers without any adverse effect on animal production performance, milk composition blood biochemical profile and economics of feeding.

Yield of glyphosate-resistant sugar beets and efficiency of weed management systems with glyphosate and conventional herbicides under German and Polish crop production.

PubMed

Nichterlein, Henrike; Matzk, Anja; Kordas, Leszek; Kraus, Josef; Stibbe, Carsten

2013-08-01

In sugar beet production, weed control is one of the most important and most expensive practices to ensure yield. Since glyphosate-resistant sugar beets are not yet approved for cultivation in the EU, little commercial experience exists with these sugar beets in Europe. Experimental field trials were conducted at five environments (Germany, Poland, 2010, 2011) to compare the effects of glyphosate with the effects of conventional weed control programs on the development of weeds, weed control efficiency and yield. The results show that the glyphosate weed control programs compared to the conventional methods decreased not only the number of herbicide applications but equally in magnitude decreased the dosage of active ingredients. The results also showed effective weed control with glyphosate when the weed covering was greater and sugar beets had a later growth stage of four true leaves. Glyphosate-resistant sugar beets applied with the glyphosate herbicide two or three times had an increase in white sugar yield from 4 to 18 % in comparison to the high dosage conventional herbicide systems. In summary, under glyphosate management sugar beets can positively contribute to the increasingly demanding requirements regarding efficient sugar beet cultivation and to the demands by society and politics to reduce the use of chemical plant protection products in the environment.

Sugar beet proteinase inhibitor (BvSTI) gene promoter is regulated by insects and wounding in transgenic Nicotiana benthamiana

USDA-ARS?s Scientific Manuscript database

A regulatory sequence from a serine proteinase inhibitor gene (BvSTIpro) shown to be up-regulated in resistant interactions with a root pest of sugar beet, the sugar beet root maggot, was fused to the ß-glucuronidase (GUS) reporter gene to characterize its expression patterns in transgenic Nicotiana...

Effect of sugar beet tubers as a partial replacer to green fodder on production performance and economics of lactating Surti buffaloes in lean period

PubMed Central

Sorathiya, L. M.; Patel, M. D.; Tyagi, K. K.; Fulsoundar, A. B.; Raval, A. P.

2015-01-01

Aim: The objective of this study was to evaluate the effects of sugar beet tubers as a replacer to green fodder on production performance and economics of lactating Surti buffaloes. Materials and Methods: This trial was conducted at the Livestock Research Station, Navsari Agricultural University, Navsari. Twenty lactating Surti buffaloes in a changeover experimental design were selected to assess the effects of replacing green fodder with sugar beet (Beta vulgaris L.) tubers on production performance, economics of feeding sugar beet and blood biochemical profile. Half (50%) of the hybrid Napier was replaced with sliced sugar beet tubers in the ration of experimental animals. Results: Partial replacement of hybrid Napier with that of sugar beet tubers numerically improved dry matter intake, milk yield, 4% fat corrected milk and milk composition parameters such as fat, solid non-fat, protein and lactose, but not significantly. The blood parameters were in normal range and non-significant except that of glucose and triglycerides, which were increased in the sugar beet group. Replacing sugar beet tubers also proved to be cost-effective with improved net profit around Rs. 6.63/day. Conclusion: It can be concluded that 50% hybrid Napier fodder can be replaced with sugar beet tubers without any adverse effect on animal production performance, milk composition blood biochemical profile and economics of feeding. PMID:27046988

Enzyme resistant carbohydrate based micro-scale materials from sugar beet (Beta vulgaris L.) pulp for food and pharmaceutical applications

USDA-ARS?s Scientific Manuscript database

Bio-based micro scale materials are increasingly used in functional food and pharmaceutical applications. The present study produced carbohydrate-based micro scale tubular materials from sugar beet (Beta vulgaris L.) pulp (SBP), a by-product of sugar beet processing. The isolated carbohydrates wer...

Comparison of ITS sequences from UK and North American sugar-beet powdery mildews and the designation of Erysiphe betae.

PubMed

Francis, Sally A; Roden, Brett C; Adams, Michael J; Weiland, John; Asher, Michael J C

2007-02-01

Powdery mildew of sugar beet, a disease of major economic significance, was first described at the beginning of the 20th century, and since then there has been some confusion over the correct taxonomic identity of the causal agent. In Europe, the fungus was initially classified as the novel species Microsphaera betae, later re-named Erysiphe betae, whilst in America it was identified as E. polygoni, despite sugar-beet isolates from both regions having a host range restricted to Beta species. It is possible that more than one fungus causes the disease, as published descriptions of conidiogenesis have differed. In this study, isolates of the fungus collected from sugar beet in the UK and USA were investigated for polymorphisms in the rDNA ITS region to determine if the same species caused the disease in both countries, whether there was any justification for the retention of the name E. polygoni in the USA, and to search for evidence of a second species infecting sugar beet. From a total of 18 isolates examined, 23 ITS sequences were obtained. Fifteen of these, which included the UK and USA isolates, were identical and the remainder had single-base substitutions, indicating that the fungi were conspecific. Dendrogram analysis of Erysiphales ITS regions revealed that the UK and North American isolates were more closely related to E. heraclei than to E. polygoni. It is proposed that the species name Erysiphe betae be used for the powdery mildew fungus that infects sugar beet. No evidence was found in this study for a second sugar-beet powdery mildew species.

Storage rot in sugar beet: variable response over time and with different host germplasm

USDA-ARS?s Scientific Manuscript database

Sugar beet (Beta vulgaris) is commonly stored in outdoor piles prior to processing for food and animal feed. While in storage the crop is subject to multiple post-harvest rots. In the Michigan growing region, little loss due to storage rots is observed until beets have been in storage for several mo...

Virulence of Rhizoctonia solani AG2-2 isolates on sugar beet (Beta vulgaris) in response to low temperature

USDA-ARS?s Scientific Manuscript database

Rhizoctonia solani AG2-2 is not only the causal agent of Rhizoctonia root and crown rot in sugar beet (Beta vulgaris) but it can also cause a seedling damping-off. Significant losses can occur in all regions where sugar beets are grown. One recommendation for managing seedling losses to R. solani is...

Hyper sausage neuron: Recognition of transgenic sugar-beet based on terahertz spectroscopy

NASA Astrophysics Data System (ADS)

Liu, Jianjun; Li, Zhi; Hu, Fangrong; Chen, Tao; Du, Yong; Xin, Haitao

2015-01-01

This paper presents a novel approach for identification of terahertz (THz) spectral of genetically modified organisms (GMOs) based on Hyper Sausage Neuron (HSN), and THz transmittance spectra of some typical transgenic sugar-beet samples are investigated to demonstrate its feasibility. Principal component analysis (PCA) is applied to extract features of the spectrum data, and instead of the original spectrum data, the feature signals are fed into the HSN pattern recognition, a new multiple weights neural network (MWNN). The experimental result shows that the HSN model not only can correctly classify different types of transgenic sugar-beets, but also can reject identity non similar samples in the same type. The proposed approach provides a new effective method for detection and identification of GMOs by using THz spectroscopy.

Comparison of Spinach Sex Chromosomes with Sugar Beet Autosomes Reveals Extensive Synteny and Low Recombination at the Male-Determining Locus.

PubMed

Takahata, Satoshi; Yago, Takumi; Iwabuchi, Keisuke; Hirakawa, Hideki; Suzuki, Yutaka; Onodera, Yasuyuki

2016-01-01

Spinach (Spinacia oleracea, 2n = 12) and sugar beet (Beta vulgaris, 2n = 18) are important crop members of the family Chenopodiaceae ss Sugar beet has a basic chromosome number of 9 and a cosexual breeding system, as do most members of the Chenopodiaceae ss. family. By contrast, spinach has a basic chromosome number of 6 and, although certain cultivars and genotypes produce monoecious plants, is considered to be a dioecious species. The loci determining male and monoecious sexual expression were mapped to different loci on the spinach sex chromosomes. In this study, a linkage map with 46 mapped protein-coding sequences was constructed for the spinach sex chromosomes. Comparison of the linkage map with a reference genome sequence of sugar beet revealed that the spinach sex chromosomes exhibited extensive synteny with sugar beet chromosomes 4 and 9. Tightly linked protein-coding genes linked to the male-determining locus in spinach corresponded to genes located in or around the putative pericentromeric and centromeric regions of sugar beet chromosomes 4 and 9, supporting the observation that recombination rates were low in the vicinity of the male-determining locus. The locus for monoecism was confined to a chromosomal segment corresponding to a region of approximately 1.7Mb on sugar beet chromosome 9, which may facilitate future positional cloning of the locus. © The American Genetic Association 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

A SNP mutation affects rhizomania-virus content of sugar beets grown on resistance-breaking soi

USDA-ARS?s Scientific Manuscript database

Rhizomania is one of the most devastating biotic stresses affecting sugar beet (Beta vulgaris L.). It is caused by Beet necrotic yellow vein virus (BNYVV) vectored by the plasmodiophorid Polymyxa betae K. The only means available to control the disease is the use of genetically resistant varieties. ...

Metabolome profiling to understand the defense response to sugar beet (Beta vulgaris) to Rhizoctonia solani AG 2-2 IIIB

USDA-ARS?s Scientific Manuscript database

Rhizoctonia crown and root rot, caused by Rhizoctonia solani Kühn AG 2-2 IIIB, is an important disease of sugar beet (Beta vulgaris L.). The molecular processes that mediate sugar beet resistance to R. solani are largely unknown and identifying the metabolites associated with R. solani infection ma...

Potassium Uptake Efficiency and Dynamics in the Rhizosphere of Maize, Wheat, and Sugar Beet Evaluated with a Mechanistic Model

USDA-ARS?s Scientific Manuscript database

Plant species differ in nutrient uptake efficiency. With a pot experiment, we evaluated potassium (K) uptake efficiency of maize (Zea mays L.), wheat (Triticum aestivum L.), and sugar beet (Beta vulgaris L.) grown on a low-K soil. Sugar beet and wheat maintained higher shoot K concentrations, indica...

Length of efficacy for control of curly top in sugar beet with seed foliar insecticides

USDA-ARS?s Scientific Manuscript database

Curly top in sugar beet caused by Beet curly top virus (BCTV) is an important yield limiting disease that can be reduced via neonicotinoid and pyrethroid insecticides. However the length of efficacy of these insecticides is poorly understood, so a series of field experiments was conducted with the ...

Sugar beet cell wall protein confers fungal and pest resistance in genetically engineered plants

USDA-ARS?s Scientific Manuscript database

Sugar beet biomass and sugar yield are reduced by diseases caused by microbial pathogens and insect pest infestations. Since disease and pest control measures continue to rely on harmful chemical fungicides and insecticides, biotechnological approaches offer an alternate approach for disease and pe...

Identification of Beet necrotic yellow vein virus P25 pathogenicity factor-interacting sugar beet proteins that represent putative virus targets or components of plant resistance.

PubMed

Thiel, Heike; Varrelmann, Mark

2009-08-01

Beet necrotic yellow vein virus (BNYVV) induces the most important disease threatening sugar beet. The growth of partially resistant hybrids carrying monogenic dominant resistance genes stabilize yield but are unable to entirely prevent virus infection and replication. P25 is responsible for symptom development and previous studies have shown that recently occurring resistance-breaking isolates possess increased P25 variability. To better understand the viral pathogenicity factor's interplay with plant proteins and to possibly unravel the molecular basis of sugar beet antivirus resistance, P25 was applied in a yeast two-hybrid screen of a resistant sugar beet cDNA library. This screen identified candidate proteins recognized as orthologues from other plant species which are known to be expressed following pathogen infection and involved in plant defense response. Most of the candidates potentially related to host-pathogen interactions were involved in the ubiquitylation process and plants response to stress, and were part of cell and metabolism components. The interaction of several candidate genes with P25 was confirmed in Nicotiana benthamiana leaf cells by transient agrobacterium-mediated expression applying bimolecular fluorescence complementation assay. The putative functions of several of the candidates identified support previous findings and present first targets for understanding the BNYVV pathogenicity and antivirus resistance mechanism.

Physico-chemical characterization of a cellulosic fraction from sugar beet pulp

USDA-ARS?s Scientific Manuscript database

The residue of sugar beet pulp from which pectin and alkaline soluble polysaccharides have been removed by microwave assisted extraction (MAE) or conventional heat was treated with sodium monochloroacetate under alkaline pH to convert the residual cellulose present to carboxy methyl cellulose (CMC)....

High-throughput RAD-SNP genotyping for characterization of sugar beet genotypes

USDA-ARS?s Scientific Manuscript database

High-throughput SNP genotyping provides a rapid way of developing resourceful set of markers for delineating the genetic architecture and for effective species discrimination. In the presented research, we demonstrate a set of 192 SNPs for effective genotyping in sugar beet using high-throughput mar...

The characterization of sugar beet pectin using the EcoSEC® GPC system coupled to multi-angle light scattering, quasi-elastic light scattering, and differential viscometry

USDA-ARS?s Scientific Manuscript database

The need to increase the use of low valued co-products derived from the processing of sugar beets has prompted the investigation of the structure of the pectin extracted from sugar beet pulp. The characterization of sugar beet pectin is essential as it has the potential to be used in the production ...

Effect of Cuscuta campestris parasitism on the physiological and anatomical changes in untreated and herbicide-treated sugar beet.

PubMed

Saric-Krsmanovic, Marija M; Bozic, Dragana M; Radivojevic, Ljiljana M; Umiljendic, Jelena S Gajic; Vrbnicanin, Sava P

2017-11-02

The effects of field dodder on physiological and anatomical processes in untreated sugar beet plants and the effects of propyzamide on field dodder were examined under controlled conditions. The experiment included the following variants: N-noninfested sugar beet plants (control); I - infested sugar beet plants (untreated), and infested plants treated with propyzamide (1500 g a.i. ha -1 (T 1 ) and 2000 g a.i. ha -1 (T 2 )). The following parameters were checked: physiological-pigment contents (chlorophyll a, chlorophyll b, total carotenoids); anatomical -leaf parameters: thickness of epidermis, parenchyma and spongy tissue, mesophyll and underside leaf epidermis, and diameter of bundle sheath cells; petiole parameters: diameter of tracheid, petiole hydraulic conductance, xylem surface, phloem cell diameter and phloem area in sugar beet plants. A conventional paraffin wax method was used to prepare the samples for microscopy. Pigment contents were measured spectrophotometrically after methanol extraction. All parameters were measured: prior to herbicide application (0 assessment), then 7, 14, 21, 28 and 35 days after application (DAA). Field dodder was found to affect the pigment contents in untreated sugar beet plants, causing significant reductions. Conversely, reduction in the treated plants decreased 27% to 4% for chlorophyll a, from 21% to 5% for chlorophyll b, and from 28% to 5% for carotenoids (T 1 ). Also, in treatment T 2, reduction decreased in infested and treated plants from 19% to 2% for chlorophyll a, from 21% to 2% for chlorophyll b, from 23% to 3% for carotenoids and stimulation of 1% and 2% was observed 28 and 35 DAA, respectively. Plants infested (untreated) by field dodder had lower values of most anatomical parameters, compared to noninfested plants. The measured anatomical parameters of sugar beet leaves and petiole had significantly higher values in noninfested plants and plants treated with propyzamide than in untreated plants. Also, the

Effect of Environment and Sugar Beet Genotype on Root Rot Development and Pathogen Profile During Storage.

PubMed

Liebe, Sebastian; Varrelmann, Mark

2016-01-01

Storage rots represent an economically important factor impairing the storability of sugar beet by increasing sucrose losses and invert sugar content. Understanding the development of disease management strategies, knowledge about major storage pathogens, and factors influencing their occurrence is crucial. In comprehensive storage trials conducted under controlled conditions, the effects of environment and genotype on rot development and associated quality changes were investigated. Prevalent species involved in rot development were identified by a newly developed microarray. The strongest effect on rot development was assigned to environment factors followed by genotypic effects. Despite large variation in rot severity (sample range 0 to 84%), the spectrum of microorganisms colonizing sugar beet remained fairly constant across all treatments with dominant species belonging to the fungal genera Botrytis, Fusarium, and Penicillium. The intensity of microbial tissue necrotization was strongly correlated with sucrose losses (R² = 0.79 to 0.91) and invert sugar accumulation (R² = 0.91 to 0.95). A storage rot resistance bioassay was developed that could successfully reproduce the genotype ranking observed in storage trials. Quantification of fungal biomass indicates that genetic resistance is based on a quantitative mechanism. Further work is required to understand the large environmental influence on rot development in sugar beet.

Molecular markers for improving control of soil-borne pathogen Fusarium oxysporum in sugar beet

USDA-ARS?s Scientific Manuscript database

Fusarium oxysporum f. sp. betae (FOB) is an important pathogen of sugar beet worldwide causing leaf yellowing and vascular discoloration. The use of tolerant varieties is one of the most effective methods for managing this disease. In this study, a large germplasm collection,comprised of 29 sugar be...

Multilocus analysis using putative fungal effectors to describe a population of Fusarium oxysporum from sugar beet.

PubMed

Covey, Paul A; Kuwitzky, Brett; Hanson, Mia; Webb, Kimberly M

2014-08-01

Sugar beet (Beta vulgaris) Fusarium yellows is caused by Fusarium oxysporum f. sp. betae and can lead to significant reductions in root yield, sucrose percentage, juice purity, and storability. F. oxysporum f. sp. betae can be highly variable and many F. oxysporum strains isolated from symptomatic sugar beet are nonpathogenic. Identifying pathogenicity factors and their diversity in the F. oxysporum f. sp. betae population could further understanding of how this pathogen causes disease and potentially provide molecular markers to rapidly identify pathogenic isolates. This study used several previously described fungal effector genes (Fmk1, Fow1, Pda1, PelA, PelD, Pep1, Prt1, Rho1, Sge1, Six1, Six6, Snf1, and Ste12) as genetic markers, in a population of 26 pathogenic and nonpathogenic isolates of F. oxysporum originally isolated from symptomatic sugar beet. Of the genes investigated, six were present in all F. oxysporum isolates from sugar beet (Fmk1, Fow1, PelA, Rho1, Snf1, and Ste12), and seven were found to be dispersed within the population (Pda1, PelD, Pep1, Prt1, Sge1, Six1, and Six6). Of these, Fmk1, Fow1, PelA, Rho1, Sge1, Snf1, and Ste12 were significant in relating clade designations and PelD, and Prt1 were significant for correlating with pathogenicity in F. oxysporum f. sp. betae.

Development of a DNA Microarray-Based Assay for the Detection of Sugar Beet Root Rot Pathogens.

PubMed

Liebe, Sebastian; Christ, Daniela S; Ehricht, Ralf; Varrelmann, Mark

2016-01-01

Sugar beet root rot diseases that occur during the cropping season or in storage are accompanied by high yield losses and a severe reduction of processing quality. The vast diversity of microorganism species involved in rot development requires molecular tools allowing simultaneous identification of many different targets. Therefore, a new microarray technology (ArrayTube) was applied in this study to improve diagnosis of sugar beet root rot diseases. Based on three marker genes (internal transcribed spacer, translation elongation factor 1 alpha, and 16S ribosomal DNA), 42 well-performing probes enabled the identification of prevalent field pathogens (e.g., Aphanomyces cochlioides), storage pathogens (e.g., Botrytis cinerea), and ubiquitous spoilage fungi (e.g., Penicillium expansum). All probes were proven for specificity with pure cultures from 73 microorganism species as well as for in planta detection of their target species using inoculated sugar beet tissue. Microarray-based identification of root rot pathogens in diseased field beets was successfully confirmed by classical detection methods. The high discriminatory potential was proven by Fusarium species differentiation based on a single nucleotide polymorphism. The results demonstrate that the ArrayTube constitute an innovative tool allowing a rapid and reliable detection of plant pathogens particularly when multiple microorganism species are present.

Sugar Beets, Segregation, and Schools: Mexican Americans in a Northern Colorado Community, 1920-1960.

ERIC Educational Resources Information Center

Donato, Ruben

2003-01-01

What was unique about the Mexican American experience in Fort Collins (Colorado) was the extent to which the Great Western Sugar Company colonized Mexican workers. They lived in Mexican colonies, separate neighborhoods, or remote locations on sugar beet farms. In public schools, Mexican Americans were perceived as intellectually inferior and were…

Environmental implications of gene flow from sugar beet to wild beet--current status and future research needs.

PubMed

Bartsch, Detlef; Cuguen, Joel; Biancardi, Enrico; Sweet, Jeremy

2003-01-01

Gene flow via seed or pollen is a basic biological process in plant evolution. The ecological and genetic consequences of gene flow depend on the amount and direction of gene flow as well as on the fitness of hybrids. The assessment of potential risks of transgenic plants should take into account the fact that conventional crops can often cross with wild plants. The precautionary approach in risk management of genetically modified plants (GMPs) may make it necessary to monitor significant wild and weed populations that might be affected by transgene escape. Gene flow is hard to control in wind-pollinated plants like beet (Beta vulgaris). In addition, wild beet populations potentially can undergo evolutionary changes which might expand their geographical distribution. Unintended products of cultivated beets pollinated by wild beets are weed beets that bolt and flower during their first year of planting. Weed beets cause yield losses and can delay harvest. Wild beets are important plant genetic resources and the preservation of wild beet diversity in Europe has been considered in biosafety research. We present here the methodology and research approaches that can be used for monitoring the geographical distribution and diversity of Beta populations. It has recently been shown that a century of gene flow from Beta vulgaris ssp. vulgaris has not altered the genetic diversity of wild Beta vulgaris L. ssp. maritima (L.) Arcang. in the Italian sugar beet seed production area. Future research should focus on the potential evolution of transgenic wild beet populations in comparison to these baseline data. Two monitoring models are presented describing how endpoints can be measured: (1) "Pre-post" crop commercialization against today's baseline and (2) "Parallel" to crop commercialization against GMP free reference areas/ populations. Model 2 has the advantage of taking ongoing changes in genetic diversity and population dynamics into account. Model 1 is more applicable if

Semiquantitative determination of short-chain fatty acids in cane and beet sugars.

PubMed

Batista, Rebecca B; Grimm, Casey C; Godshall, Mary An

2002-03-01

Some sugars, specifically white beet sugar and raw cane sugars, possess off-flavors and off-odors. Although not necessarily the source, the presence of short-chain fatty acids serves as an indicator of an off-odor problem in sugar. Solid-phase microextraction (SPME) is used to collect the volatile compounds from the headspace of sugar. The temperature, moisture, and type of SPME fiber are varied to optimize recovery. Sugars analyzed in the absence of water using an incubation temperature of 70 degrees C with a divinylbenzene-carboxen-polydimethylsiloxane fiber yield the most reproducible results. Data from depletion analyses report a recovery level of 38% for the first injection. The semiquantitative analysis of butyric acid is accomplished using injected standards to develop a calibration curve.

System dynamics approach for modeling of sugar beet yield considering the effects of climatic variables.

PubMed

Pervin, Lia; Islam, Md Saiful

2015-02-01

The aim of this study was to develop a system dynamics model for computation of yields and to investigate the dependency of yields on some major climatic parameters, i.e. temperature and rainfall, for Beta vulgaris subsp. (sugar beet crops) under future climate change scenarios. A system dynamics model was developed which takes account of the effects of rainfall and temperature on sugar beet yields under limited irrigation conditions. A relationship was also developed between the seasonal evapotranspiration and seasonal growing degree days for sugar beet crops. The proposed model was set to run for the present time period of 1993-2012 and for the future period 2013-2040 for Lethbridge region (Alberta, Canada). The model provides sugar beet yields on a yearly basis which are comparable to the present field data. It was found that the future average yield will be increased at about 14% with respect to the present average yield. The proposed model can help to improve the understanding of soil water conditions and irrigation water requirements of an area under certain climatic conditions and can be used for future prediction of yields for any crops in any region (with the required information to be provided). The developed system dynamics model can be used as a supporting tool for decision making, for improvement of agricultural management practice of any region. © 2014 Society of Chemical Industry.

Effects of beer factory sludge on soil properties and growth of sugar beet (Beta vulgaris saccharifera L.).

PubMed

Kütük, Cihat; Cayci, Gökhan; Baran, Abdullah; Başkan, Oguz; Hartmann, Roger

2003-10-01

The possible use of beer factory sludge (BFS) for an agricultural purpose was investigated with sugar beet (Beta vulgaris saccharifera L.). BFS was air dried and sieved through a 4 mm mesh before application to a soil (Typic Xerofluvent). Afterwards, the BFS was mixed with soil at a rate 0, 10, 20, 40, 80 and 160 tonnes ha(-1). The mixtures were than put into pots and kept in the greenhouse for an incubation of five months. During the incubation, pH, the electrical conductivity, the organic matter content, NH4+-N and NO3--N content were regularly measured. At the end of the incubation period, sugar beet seeds were sown into the same pots. After a growing period of six-months the sugar beet plants were harvested, and yield and quality parameters were determined. BFS increased leaf and root yield. However, the effect of BFS on leaf growth was more pronounced than on root growth. The highest sugar content, refined sugar content and refined sugar yield were obtained with the application rate of 10 tonnes BFS per hectare. Ten tonnes of BSF per hectare was the most suitable on the basis of root quality parameters and root yield. However BFS should be applied to the soil six or seven months in advance due to the high level of nitrogen released through mineralization.

Adaptation of Rhizobium leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics

PubMed Central

2011-01-01

Background The rhizosphere is the microbe-rich zone around plant roots and is a key determinant of the biosphere's productivity. Comparative transcriptomics was used to investigate general and plant-specific adaptations during rhizosphere colonization. Rhizobium leguminosarum biovar viciae was grown in the rhizospheres of pea (its legume nodulation host), alfalfa (a non-host legume) and sugar beet (non-legume). Gene expression data were compared to metabolic and transportome maps to understand adaptation to the rhizosphere. Results Carbon metabolism was dominated by organic acids, with a strong bias towards aromatic amino acids, C1 and C2 compounds. This was confirmed by induction of the glyoxylate cycle required for C2 metabolism and gluconeogenesis in all rhizospheres. Gluconeogenesis is repressed in R. leguminosarum by sugars, suggesting that although numerous sugar and putative complex carbohydrate transport systems are induced in the rhizosphere, they are less important carbon sources than organic acids. A common core of rhizosphere-induced genes was identified, of which 66% are of unknown function. Many genes were induced in the rhizosphere of the legumes, but not sugar beet, and several were plant specific. The plasmid pRL8 can be considered pea rhizosphere specific, enabling adaptation of R. leguminosarum to its host. Mutation of many of the up-regulated genes reduced competitiveness for pea rhizosphere colonization, while two genes specifically up-regulated in the pea rhizosphere reduced colonization of the pea but not alfalfa rhizosphere. Conclusions Comparative transcriptome analysis has enabled differentiation between factors conserved across plants for rhizosphere colonization as well as identification of exquisite specific adaptation to host plants. PMID:22018401

Land application of sugar beet by-products: effects on runoff and percolating water quality.

PubMed

Kumar, Kuldip; Rosen, Carl J; Gupta, Satish C; McNearney, Matthew

2009-01-01

Water quality concerns, including greater potential for nutrient transport to surface waters resulting in eutrophication and nutrient leaching to ground water, exist when agricultural or food processing industry wastes and by-products are land applied. Plot- and field-scale studies were conducted to evaluate the effects of sugar beet by-products on NO3-N and P losses and biochemical oxygen demand (BOD) in runoff and NO3-N concentrations in percolating waters. In the runoff plot study, treatments in the first year included two rates (224 and 448 Mg ha(-1) fresh weight) of pulp and spoiled beets and a nonfertilized control. In the second year, no by-products were applied on the treated plots, the control treatment was fertilized with N fertilizer, and an additional treatment was added as a nonfertilized control in buffer areas. Wheat (Triticum aestivum L.) was grown in the year of by-product application and sugar beet (Beta vulgaris L.) in the following year. In the percolation field study, the treatments were the control, pulp (224 Mg ha(-)(1)), and spoiled beets (224 Mg ha(-1)). Results from the runoff plot showed that both by-products caused immobilization of soil inorganic N and thus reduced NO3-N losses in runoff and soil waters during the first growing season. There was some risk of NO3-N exceeding the drinking water limit of 10 mg L(-1), especially between the period of wheat harvest and soil freezing in fall when pulp was applied at 448 Mg ha(-1). The field-scale study showed that by-product application at 224 Mg ha(-1) did not result in increased ground water NO3-N concentrations. Application of spoiled beets at both rates caused significantly higher BODs in runoff in the first year of application. The concentrations of total and soluble reactive P (SRP) were also higher from both rates of spoiled beet application and from the higher application rate of pulp during the 2-yr study period. These high BODs and total P and SRP concentrations in runoff waters

Haplotype Variation of Flowering Time Genes of Sugar Beet and Its Wild Relatives and the Impact on Life Cycle Regimes.

PubMed

Höft, Nadine; Dally, Nadine; Hasler, Mario; Jung, Christian

2017-01-01

The species Beta vulgaris encompasses wild and cultivated members with a broad range of phenological development. The annual life cycle is commonly found in sea beets (ssp. maritima ) from Mediterranean environments which germinate, bolt, and flower within one season under long day conditions. Biennials such as the cultivated sugar beet ( B. vulgaris ssp. vulgaris ) as well as sea beets from northern latitudes require prolonged exposure to cold temperature over winter to acquire floral competence. Sugar beet is mainly cultivated for sugar production in Europe and is likely to have originated from sea beet. Flowering time strongly affects seed yield and yield potential and is thus a trait of high agronomic relevance. Besides environmental cues, there are complex genetic networks known to impact life cycle switch in flowering plants. In sugar beet, BTC1, BvBBX19, BvFT1 , and BvFT2 are major flowering time regulators. In this study, we phenotyped plants from a diversity Beta panel encompassing cultivated and wild species from different geographical origin. Plants were grown under different day length regimes with and without vernalization. Haplotype analysis of BTC1, BvBBX19, BvFT1 , and BvFT2 was performed to identify natural diversity of these genes and their impact on flowering. We found that accessions from northern latitudes flowered significantly later than those from southern latitudes. Some plants did not flower at all, indicating a strong impact of latitude of origin on life cycle. Haplotype analysis revealed a high conservation of the CCT-, REC-, BBX-, and PEBP-domains with regard to SNP occurrence. We identified sequence variation which may impact life cycle adaptation in beet. Our data endorse the importance of BTC1 in the domestication process of cultivated beets and contribute to the understanding of distribution and adaption of Beta species to different life cycle regimes in response to different environments. Moreover, our data provide a resource for

Development of real-time PCR method for the detection and the quantification of a new endogenous reference gene in sugar beet "Beta vulgaris L.": GMO application.

PubMed

Chaouachi, Maher; Alaya, Akram; Ali, Imen Ben Haj; Hafsa, Ahmed Ben; Nabi, Nesrine; Bérard, Aurélie; Romaniuk, Marcel; Skhiri, Fethia; Saïd, Khaled

2013-01-01

KEY MESSAGE : Here, we describe a new developed quantitative real-time PCR method for the detection and quantification of a new specific endogenous reference gene used in GMO analysis. The key requirement of this study was the identification of a new reference gene used for the differentiation of the four genomic sections of the sugar beet (Beta vulgaris L.) (Beta, Corrollinae, Nanae and Procumbentes) suitable for quantification of genetically modified sugar beet. A specific qualitative polymerase chain reaction (PCR) assay was designed to detect the sugar beet amplifying a region of the adenylate transporter (ant) gene only from the species of the genomic section I of the genus Beta (cultivated and wild relatives) and showing negative PCR results for 7 species of the 3 other sections, 8 related species and 20 non-sugar beet plants. The sensitivity of the assay was 15 haploid genome copies (HGC). A quantitative real-time polymerase chain reaction (QRT-PCR) assay was also performed, having high linearity (R (2) > 0.994) over sugar beet standard concentrations ranging from 20,000 to 10 HGC of the sugar beet DNA per PCR. The QRT-PCR assay described in this study was specific and more sensitive for sugar beet quantification compared to the validated test previously reported in the European Reference Laboratory. This assay is suitable for GMO quantification in routine analysis from a wide variety of matrices.

Influence of Rhizoctonia-Bacterial root rot complex on storability of sugar beet

USDA-ARS?s Scientific Manuscript database

The root rot complex, caused by Rhizoctonia solani and Leuconostoc mesenteroides, can lead to yield loss in the field but may also lead to problems with sucrose loss in storage. Thus, studies were conducted to investigate if placing sugar beet roots suffering from root rot together with healthy roo...

Postharvest respiration rate and sucrose concentration of Rhizoctonia-infected sugar beet roots

USDA-ARS?s Scientific Manuscript database

Rhizoctonia crown and root rot (RCRR), caused by Rhizoctonia solani AG 2-2, is a common root disease on sugar beet that reduces yield and sucrose during the growing season and causes further losses by increasing respiration and reducing sucrose content during storage. The industry needs to identify...

Modified sugar beet pectin induces apoptosis of colon cancer cells via interaction with the neutral sugar side-chains

USDA-ARS?s Scientific Manuscript database

Pectins extracted from a variety of sources and modified with heat and/or pH have previously been shown to exhibit activity towards several cancer cell lines. However, the structural basis for the anti-cancer activity of modified pectin requires clarification. Sugar beet and citrus pectin extracts h...

Properties of Two Novel Esterases Identified from Culture Supernatant of Penicillium purpurogenum Grown on Sugar Beet Pulp.

PubMed

Oleas, Gabriela; Callegari, Eduardo; Sepulveda, Romina; Eyzaguirre, Jaime

2016-01-01

The filamentous fungus Penicillium purpurogenum grows on a variety of natural carbon sources, such as sugar beet pulp, and secretes to the medium a large number of enzymes that degrade the carbohydrate components of lignocellulose. Sugar beet pulp is rich in pectin, and the purpose of this work is to identify novel esterases produced by the fungus, which may participate in pectin degradation. Partially purified culture supernatants of the fungus grown on sugar beet pulp were subjected to mass spectrometry analysis. Peptides thus identified, which may be part of potential esterases were probed against the proteins deduced from the fungal genome sequence. The cDNAs of two putative esterases identified were expressed in Pichia pastoris and their properties studied. One of these enzymes, named FAET, is a feruloyl esterase, while the other, PE, is classified as a pectin methyl esterase. These findings add to our knowledge of the enzymology of pectin degradation by Penicillium purpurogenum, and define properties of two novel esterases acting on de-esterification of pectin. Their availability may be useful as tools for the study of pectin structure and degradation.

Real-Time Blob-Wise Sugar Beets VS Weeds Classification for Monitoring Fields Using Convolutional Neural Networks

NASA Astrophysics Data System (ADS)

Milioto, A.; Lottes, P.; Stachniss, C.

2017-08-01

UAVs are becoming an important tool for field monitoring and precision farming. A prerequisite for observing and analyzing fields is the ability to identify crops and weeds from image data. In this paper, we address the problem of detecting the sugar beet plants and weeds in the field based solely on image data. We propose a system that combines vegetation detection and deep learning to obtain a high-quality classification of the vegetation in the field into value crops and weeds. We implemented and thoroughly evaluated our system on image data collected from different sugar beet fields and illustrate that our approach allows for accurately identifying the weeds on the field.

Utilization of concentrate after membrane filtration of sugar beet thin juice for ethanol production.

PubMed

Kawa-Rygielska, Joanna; Pietrzak, Witold; Regiec, Piotr; Stencel, Piotr

2013-04-01

The subject of this study was to investigate the feasibility of the concentrate obtained after membrane ultrafiltration of sugar beet thin juice for ethanol production and selection of fermentation conditions (yeast strain and media supplementation). Resulting concentrate was subjected to batch ethanol fermentation using two strains of Saccharomyces cerevisiae (Ethanol Red and Safdistill C-70). The effect of different forms of media supplementation (mineral salts: (NH4)2SO4, K2HPO4, MgCl2; urea+Mg3(PO4)2 and yeast extract) on the fermentation course was also studied. It was stated that sugar beet juice concentrate is suitable for ethanol production yielding, depending on the yeast strain, ca. 85-87 g L(-1) ethanol with ca. 82% practical yield and more than 95% of sugars consumption after 72 h of fermentation. Nutrients enrichment further increased ethanol yield. The best results were obtained for media supplemented with urea+Mg3(PO4)2 yielding 91.16-92.06 g L(-1) ethanol with practical yield ranging 84.78-85.62% and full sugars consumption. Copyright © 2013. Published by Elsevier Ltd.

DNA methylation of retrotransposons, DNA transposons and genes in sugar beet (Beta vulgaris L.).

PubMed

Zakrzewski, Falk; Schmidt, Martin; Van Lijsebettens, Mieke; Schmidt, Thomas

2017-06-01

The methylation of cytosines shapes the epigenetic landscape of plant genomes, coordinates transgenerational epigenetic inheritance, represses the activity of transposable elements (TEs), affects gene expression and, hence, can influence the phenotype. Sugar beet (Beta vulgaris ssp. vulgaris), an important crop that accounts for 30% of worldwide sugar needs, has a relatively small genome size (758 Mbp) consisting of approximately 485 Mbp repetitive DNA (64%), in particular satellite DNA, retrotransposons and DNA transposons. Genome-wide cytosine methylation in the sugar beet genome was studied in leaves and leaf-derived callus with a focus on repetitive sequences, including retrotransposons and DNA transposons, the major groups of repetitive DNA sequences, and compared with gene methylation. Genes showed a specific methylation pattern for CG, CHG (H = A, C, and T) and CHH sites, whereas the TE pattern differed, depending on the TE class (class 1, retrotransposons and class 2, DNA transposons). Along genes and TEs, CG and CHG methylation was higher than that of adjacent genomic regions. In contrast to the relatively low CHH methylation in retrotransposons and genes, the level of CHH methylation in DNA transposons was strongly increased, pointing to a functional role of asymmetric methylation in DNA transposon silencing. Comparison of genome-wide DNA methylation between sugar beet leaves and callus revealed a differential methylation upon tissue culture. Potential epialleles were hypomethylated (lower methylation) at CG and CHG sites in retrotransposons and genes and hypermethylated (higher methylation) at CHH sites in DNA transposons of callus when compared with leaves. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.

Functional characterisation and cell specificity of BvSUT1, the transporter that loads sucrose into the phloem of sugar beet (Beta vulgaris L.) source leaves.

PubMed

Nieberl, P; Ehrl, C; Pommerrenig, B; Graus, D; Marten, I; Jung, B; Ludewig, F; Koch, W; Harms, K; Flügge, U-I; Neuhaus, H E; Hedrich, R; Sauer, N

2017-05-01

Sugar beet (Beta vulgaris L.) is one of the most important sugar-producing plants worldwide and provides about one third of the sugar consumed by humans. Here we report on molecular characterisation of the BvSUT1 gene and on the functional characterisation of the encoded transporter. In contrast to the recently identified tonoplast-localised sucrose transporter BvTST2.1 from sugar beet taproots, which evolved within the monosaccharide transporter (MST) superfamily, BvSUT1 represents a classical sucrose transporter and is a typical member of the disaccharide transporter (DST) superfamily. Transgenic Arabidopsis plants expressing the β-GLUCURONIDASE (GUS) reporter gene under control of the BvSUT1-promoter showed GUS histochemical staining of their phloem; an anti-BvSUT1-antiserum identified the BvSUT1 transporter specifically in phloem companion cells. After expression of BvSUT1 cDNA in bakers' yeasts (Saccharomyces cerevisiae) uptake characteristics of the BvSUT1 protein were studied. Moreover, the sugar beet transporter was characterised as a proton-coupled sucrose symporter in Xenopus laevis oocytes. Our findings indicate that BvSUT1 is the sucrose transporter that is responsible for loading of sucrose into the phloem of sugar beet source leaves delivering sucrose to the storage tissue in sugar beet taproot sinks. © 2017 German Botanical Society and The Royal Botanical Society of the Netherlands.

Experimental Sugar Beet Cultivars Evaluated for Resistance Bacterial Root Rot in Idaho, 2008

USDA-ARS?s Scientific Manuscript database

Bacterial root rot of sugar beet caused by Leuconostoc mesenteroides subsp. dextranicum is a disease problem recently described in the United States. To ameliorate the impact of bacterial root rot on sucrose loss in the field, storage piles, and factories, a study was conducted to identify resistan...

Scale up of fuel ethanol production from sugar beet juice using loofa sponge immobilized bioreactor.

PubMed

Ogbonna, J C; Mashima, H; Tanaka, H

2001-01-01

Production of fuel ethanol from sugar beet juice, using cells immobilized on loofa sponge was investigated. Based on ethanol productivity and ease of cell immobilization, a flocculating yeast strain, Saccharomyces cerevisiae IR2 was selected for ethanol production from sugar beet juice. It was found that raw sugar beet juice was an optimal substrate for ethanol production, requiring neither pH adjustment nor nitrogen source supplement. When compared with a 2 l bubble column bioreactor, mixing was not sufficient in an 8 l bioreactor containing a bed of sliced loofa sponges and consequently, the immobilized cells were not uniformly distributed within the bed. Most of the cells were immobilized in the lower part of the bed and this resulted in decreased ethanol productivity. By using an external loop bioreactor, constructing the fixed bed with cylindrical loofa sponges, dividing the bed into upper, middle and lower sections with approximately 1 cm spaces between them and circulating the broth through the loop during the immobilization, uniform cell distribution within the bed was achieved. Using this method, the system was scaled up to 50 l and when compared with the 2 l bubble column bioreactor, there were no significant differences (P > 0.05) in ethanol productivity and yield. By using external loop bioreactor to immobilize the cells uniformly on the loofa sponge beds, efficient large scale ethanol production systems can be constructed.

The measurement of mannitol in sugar beet factories to monitor deterioration and processing problems

USDA-ARS?s Scientific Manuscript database

Sugar beet deterioration can still be a major technological constraint in processing. The major (but not sole) contributor to deterioration in many countries, particularly when warm and humid conditions prevail, is infection by hetero-fermentative Leuconostoc mesenteroides lactic acid bacteria. In...

Analysis of Mannitol, as Tracer of Bacterial Infections in Cane and Beet Sugar Factories

USDA-ARS?s Scientific Manuscript database

Mannitol, formed mainly by Leuconostoc mesenteroides bacteria, is a sensitive marker of sugarcane and sugarbeet deterioration that can predict multiple processing problems. The delivery of consignments of deteriorated sugarcane or sugar beets to factories can detrimentally affect multiple process u...

Analysis of Mannitol, as Tracer of Bacterial Infections in Cane and Beet Sugar Factories

USDA-ARS?s Scientific Manuscript database

Mannitol, formed mainly by Leuconostoc mesenteroides bacteria, is a sensitive marker of sugarcane and sugarbeet deterioration that can predict multiple processing problems. The delivery of consignments of deteriorated sugarcane or sugar beets to factories can detrimentally affect multiple process un...

Expression and substrate specificity of betaine/proline transporters suggest a novel choline transport mechanism in sugar beet.

PubMed

Yamada, Nana; Sakakibara, Shota; Tsutsumi, Koichi; Waditee, Rungaroon; Tanaka, Yoshito; Takabe, Teruhiro

2011-09-15

Proline transporters (ProTs) originally described as highly selective transporters for proline, have been shown to also transport glycinebetaine (betaine). Here we examined and compared the transport properties of Bet/ProTs from betaine accumulating (sugar beet, Amaranthus, and Atriplex,) and non-accumulating (Arabidopsis) plants. Using a yeast mutant deficient for uptake of proline and betaine, it was shown that all these transporters exhibited higher affinity for betaine than proline. The uptake of betaine and proline was pH-dependent and inhibited by the proton uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP). We also investigated choline transport by using a choline transport-deficient yeast mutant. Results revealed that these transporters exhibited a higher affinity for choline uptake rather than betaine. Uptake of choline by sugar beet BvBet/ProT1 was independent of the proton gradient and the inhibition by CCCP was reduced compared with that for uptake of betaine, suggesting different proton binding properties between the transport of choline and betaine. Additionally, in situ hybridization experiments revealed the localization of sugar beet BvBet/ProT1 in phloem and xylem parenchyma cells. Copyright © 2011 Elsevier GmbH. All rights reserved.

Monitoring the efficacy and metabolism of phenylcarbamates in sugar beet and black nightshade by chlorophyll fluorescence parameters.

PubMed

Abbaspoor, Majid; Streibig, Jens C

2007-06-01

Desmedipham, phenmedipham and a 50% mixture of the two decreased the maximum quantum efficiency of photosystem II (F(v)/F(m)) and the relative changes at the J step (F(vj)) immediately after spraying in both sugar beet and black nightshade grown in the greenhouse. Sugar beet recovered more rapidly from phenmedipham and the mixture than from desmedipham. Desmedipham and the mixture irreversibly affected F(v)/F(m) and F(vj) in black nightshade at much lower doses than in sugar beet. Black nightshade recovered from phenmedipham injury at the highest dose in the first experiment (120 g AI ha(-1)) but not in the second experiment (500 g AI ha(-1)). The dry matter dose-response relationships and the energy pipeline presentation confirmed the same trend. There was a relatively good correlation between F(vj) taken 1 day after spraying and dry matter taken 2 or 3 weeks after spraying. The differential speed of herbicide metabolism between weed and crop plays an important role in herbicide selectivity and can be studied by using appropriate chlorophyll a fluorescence parameters. Copyright 2007 Society of Chemical Industry.

The America Society of Sugar Beet Technologist, advancing sugarbeet research for 75 years

USDA-ARS?s Scientific Manuscript database

The American Society of Sugar Beet Technologists (ASSBT) was created 75 years ago when a group of researchers that had been meeting informally as the Sugarbeet Roundtable adopted the constitution and by-laws that provided the basis for an organization that continues to foster the exchange of ideas a...

The American Society of Sugar Beet Technologists advancing sugarbeet research for 75 years

USDA-ARS?s Scientific Manuscript database

The American Society of Sugar Beet Technologists (ASSBT) was created 75 years ago when a group of researchers that had been meeting informally as the Sugarbeet Roundtable adopted the constitution and by-laws that provided the basis for an organization that continues to foster the exchange of ideas a...

Role of Free Space in Translocation in Sugar Beet 1

PubMed Central

Geiger, Donald R.; Sovonick, Susan A.; Shock, Terri L.; Fellows, Robert J.

1974-01-01

The involvement of the free space in phloem loading of sucrose was studied in sugar beet source leaves (Beta vulgaris, L.). Sucrose, supplied exogenously to the abraded upper surface of leaves at a concentration of 20 mm, was available for translocation at rates similar to those obtained with photosynthesis. The exogenous sucrose substituted as a source of translocate for assimilate derived from photosynthesis when the latter process was disrupted by plasmolysis of the leaf with 0.8 M mannitol. The mesophyll symplast was not completely disrupted by this treatment, however. Data from the sugar uptake experiments indicate that phloem loading can occur from the free space. Isotope trapping of labeled sugars derived from 14CO2 was used to intercept and identify sugars passing through the free space prior to phloem loading. Increased translocation rates induced by 4 mm ATP or increased light intensity were accompanied by increased trapping of sucrose but not of glucose. The data support the view that sucrose passes into the free space prior to phloem loading. Images PMID:16658995

Investigation of copper sorption by sugar beet processing lime waste.

PubMed

Ippolito, J A; Strawn, D G; Scheckel, K G

2013-01-01

In the western United States, sugar beet processing for sugar recovery generates a lime-based waste product (∼250,000 Mg yr) that has little liming value in the region's calcareous soils. This area has recently experienced an increase in dairy production, with dairies using copper (Cu)-based hoof baths to prevent hoof diseases. A concern exists regarding soil Cu accumulation because spent hoof baths may be disposed of in waste ponds, with pond waters being used for irrigation. The objective of this preliminary study was to evaluate the ability of lime waste to sorb Cu. Lime waste was mixed with increasing Cu-containing solutions (up to 100,000 mg Cu kg lime waste) at various buffered pH values (pH 6, 7, 8, and 9) and shaken over various time periods (up to 30 d). Copper sorption phenomenon was quantified using sorption maximum fitting, and the sorption mechanism was investigated using X-ray absorption spectroscopy. Results showed that sorption onto lime waste increased with decreasing pH and that the maximum Cu sorption of ∼45,000 mg kg occurred at pH 6. X-ray absorption spectroscopy indicated that Cu(OH) was the probable species present, although the precipitate existed as small multinuclear precipitates on the surface of the lime waste. Such structures may be precursors for larger surface precipitates that develop over longer incubation times. Findings suggest that sugar beet processing lime waste can viably sorb Cu from liquid waste streams, and thus it may have the ability to remove Cu from spent hoof baths. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

Beet curly top resistance in USDA-ARS Ft. Collins Germplasm, 2012

USDA-ARS?s Scientific Manuscript database

Seventeen sugar beet (Beta vulgaris L.) lines from the USDA-ARS Ft. Collins sugar beet program were screened for resistance to Beet severe curly top virus (BSCTV) and other closely related Curtovirus species in 2012. Commercial sugar beet cultivars Monohikari and HM PM90 were included as susceptibl...

First report of the stubby root nematode Paratrichodorus allius on sugar beet in Minnesota

USDA-ARS?s Scientific Manuscript database

Stubby root nematodes (Paratrichodorus and Trichodorus) are migratory ectoparasites that feed on roots, transmit tobraviruses, and cause significant crop loss. In June 2015, three soil samples from a sugar beet field near Felton (Clay County), MN were submitted to the Nematology Laboratory at North ...

75 FR 29969 - Environmental Impact Statement; Determination of Nonregulated Status of Sugar Beet Genetically...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-28

... Tolerance to the Herbicide Glyphosate AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION... genetically engineered for tolerance to the herbicide glyphosate. The petition stated that this article should...-tolerant sugar beet systems. What are the impacts of weeds, herbicide-tolerant weeds, weed management...

Biodegradable composites from polyester and sugar beet pulp with antimicrobial coating for food packaging

USDA-ARS?s Scientific Manuscript database

Totally biodegradable, double-layered antimicrobial composite Sheets were introduced for food packaging. The substrate layers of the sheets were prepared from poly (lactic acid) (PLA) and sugar beet pulp (SBP) or poly (butylene adipate-co-terephthalate (PBAT) and SBP by a twin-screw extruder. The ac...

Commercial Sugar Beet Cultivars Evaluated for Resistance to Bacterial Root Rot in Idaho, 2008

USDA-ARS?s Scientific Manuscript database

Bacterial root rot of sugar beet caused by Leuconostoc mesenteroides subsp. dextranicum is a disease problem recently described in the United States. To ameliorate the impact of bacterial root rot on sucrose loss in the field, storage piles, and factories, a study was conducted to identify resistan...

Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots.

PubMed

Cai, Daguang; Thurau, Tim; Tian, Yanyan; Lange, Tina; Yeh, Kai-Wun; Jung, Christian

2003-04-01

Sporamin, a sweet potato tuberous storage protein, is a Kunitz-type trypsin inhibitor. Its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. To test its potential as an anti-feedant for the beet cyst nematode (Heterodera schachtii Schm.), the sporamin gene SpTI-1 was introduced into sugar beet (Beta vulgaris L.) by Agrobacterium rhizogenes-mediated transformation. Twelve different hairy root clones expressing sporamin were selected for studying nematode development. Of these, 8 hairy root clones were found to show significant efficiency in inhibiting the growth and development of the female nematodes whereas 4 root clones did not show any inhibitory effects even though the SpTI-1 gene was regularly expressed in all of the tested hairy roots as revealed by northern and western analyses. Inhibition of nematode development correlated with trypsin inhibitor activity but not with the amount of sporamin expressed in hairy roots. These data demonstrate that the trypsin inhibitor activity is the critical factor for inhibiting growth and development of cyst nematodes in sugar beet hairy roots expressing the sporamin gene. Hence, the sweet potato sporamin can be used as a new and effective anti-feedant for controlling cyst nematodes offering an alternative strategy for establishing nematode resistance in crops.

Measurement of moisture, soluble solids, and sucrose content and mechanical properties in sugar beet using portable visible and near-infrared spectroscopy

USDA-ARS?s Scientific Manuscript database

Visible and near-infrared spectroscopy, coupled with partial least squares regression, was used to predict the moisture, soluble solids and sucrose content and mechanical properties of sugar beet. Interactance spectra were acquired from both intact and sliced beets, using two portable spectrometers ...

Root rot symptoms in sugar beet lines caused by Fusarium oxysporum f. sp. betae

USDA-ARS?s Scientific Manuscript database

The soil-borne fungus Fusarium oxysporum may cause both Fusarium yellows and Fusarium root rot diseases with severe yield losses in cultivated sugar beet worldwide. These two diseases cause similar foliar symptoms but different root response and have been proposed to be due to two distinct F. oxyspo...

Characteristics of on-demand biogas production by using sugar beet silage.

PubMed

Ahmed, Sharif; Kazda, Marian

2017-08-01

On-demand electricity generation can be achieved by just-in-time biogas production instantly utilized in co-generation units. For this goal, easily degradable substrates like sugar beet silage have a high potential. Potential for on-demand biogas production from co-digestion of sugar beet silage (SS) with grass silage (GS) was evaluated in two experiments at organic loading rates (OLRs) of 1.5 kgVS m -3 day -1 and 2.5 kgVS m -3 day -1 , respectively. Each experiment was fed with intermittent feeding system at 8 hrs interval at the same feedstock ratios (volatile solids based) of GS:SS-1:0, 3:1 and 1:3, respectively. Modelling by Gaussian equation was performed in order to understand the effects of SS on biogas production. Addition of sugar beet silage led to maximum biogas production within a short time, but it differed significantly depending on feedstock ratios and OLRs, respectively. At OLR 1.5 kgVS m -3 day -1 , during mono fermentation of grass silage maximum biogas production rate of 0.27 l N hr -1 was reached at 2.74 hrs. Production rate did not change at feedstock ratio of GS:SS-3:1 but increased to 0.64 l N hr -1 at GS:SS-1:3 within a shorter time span (1.58 hrs). On the contrary, at OLR of 2.5 kgVS m -3 day -1 time span between feedstock input and maximum biogas production did not differ significantly (p > 0.05) among the reactors. Biogas production rates were 0.60 l N hr -1 within 2.27 hrs and 0.82 l N hr -1 within 2.30 hrs at GS:SS-3:1 and GS:SS-1:3, respectively. Surprisingly, there was no time lag between maximum biogas and methane production rates, irrespectively of OLR. This implies that once the whole microbial community is adapted to intermittent substrate input, the metabolic products are instantly utilized through the all steps of anaerobic substrate degradation. Applying this finding opens new perspectives for on-demand biogas energy production. Copyright © 2017 Elsevier Ltd. All rights reserved.

E-Screen evaluation of sugar beet feedstuffs in a case of reduced embryo transfer efficiencies in cattle: the role of phytoestrogens and zearalenone

USDA-ARS?s Scientific Manuscript database

The E-Screen assay was used to evaluate the estrogenicity of sugar beet by-products obtained from a dairy farm experiencing low success rates of embryo transfer. The beet tailings had ~ 3 fold the estradiol equivalents of the pelleted beet pulp (3.9 and 1.2 µg estradiol equivalents or E2Eq/kg dry m...

Sugar and Other Sweeteners

NASA Astrophysics Data System (ADS)

Godshall, Mary An

Sugar and starch are among the most abundant plant products available, and large industries exist worldwide to extract and process them from agricultural sources. The world production of sugar (sucrose from cane and beet) in 2004/2005 was 142 million metric tons, raw value, 1 with 24.8 percent of that being beet sugar and 75.1 percent being cane sugar.2 The proportion of beet sugar to cane sugar has fallen steadily since about 1971, when it constituted 42.8 percent of total sugar production. The decline in total beet sugar proportion over the last ten years represents not so much a decline in beet production, which has remained in a range of 33-39 million metric tons, but rather a continued increase in cane sugar production from around 70 million metric tons in 1991 to 112 million metric tons.2 The production of total world sugar has also risen dramatically since 1971/72, when it was 71.7 million tons.3

Leuconostoc spp. associated with root rot in sugar beet and their interaction with rhizoctonia solani

USDA-ARS?s Scientific Manuscript database

Rhizoctonia root and crown is an important disease problem in sugar beet caused by Rhizoctonia solani and also shown to be associated with Leuconostoc. Since, the initial Leuconostoc studies were conducted with only a few isolates and the relationship of Leuconostoc with R. solani is poorly underst...

Dynamic variation of the microbial community structure during the long-time mono-fermentation of maize and sugar beet silage

PubMed Central

Klang, Johanna; Theuerl, Susanne; Szewzyk, Ulrich; Huth, Markus; Tölle, Rainer; Klocke, Michael

2015-01-01

This study investigated the development of the microbial community during a long-term (337 days) anaerobic digestion of maize and sugar beet silage, two feedstocks that significantly differ in their chemical composition. For the characterization of the microbial dynamics, the community profiling method terminal restriction fragment length polymorphism (TRFLP) in combination with a cloning-sequencing approach was applied. Our results revealed a specific adaptation of the microbial community to the supplied feedstocks. Based on the high amount of complex compounds, the anaerobic conversion rate of maize silage was slightly lower compared with the sugar beet silage. It was demonstrated that members from the phylum Bacteroidetes are mainly involved in the degradation of low molecular weight substances such as sugar, ethanol and acetate, the main compounds of the sugar beet silage. It was further shown that species of the genus Methanosaeta are highly sensitive against sudden stress situations such as a strong decrease in the ammonium nitrogen (NH4+-N) concentration or a drop of the pH value. In both cases, a functional compensation by members of the genera Methanoculleus and/or Methanosarcina was detected. However, the overall biomass conversion of both feedstocks proceeded efficiently as a steady state between acid production and consumption was recorded, which further resulted in an equal biogas yield. PMID:25712194

Analyzing the genomes of wild and cultivated beets

USDA-ARS?s Scientific Manuscript database

Sugar beet is an important crop plant that accounts for roughly 25% of the world's sugar production per year. We have previously shown that sugar beet has a quite narrow genetic base, presumably due to a domestication bottleneck. To increase the crop ´s stress tolerance, the introduction of desirabl...

Evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (Beta vulgaris) pectin.

PubMed

Ralet, Marie-Christine; Crépeau, Marie-Jeanne; Bonnin, Estelle

2008-06-01

Commercial acid-extracted sugar beet pectin was extensively hydrolysed using an endo-polygalacturonase (AnPGI from Aspergillus niger or AnPGII from A. niger or FmPG from Fusarium moniliforme) in combination with Aspergillus aculeatus pectin methyl-esterase (AaPME). The homogalacturonan-derived oligogalacturonates released were quantified by high-performance anion-exchange chromatography and their structure determined by mass spectrometry. The different endo-polygalacturonases exhibited variable tolerance towards acetyl groups. AnPGI was the most active and FmPG the less. A hypothetical homogalacturonan was constructed using the AnPGI-recovered oligogalacturonates as building blocks and the validity of the model was checked taking into account FmPG observed requirements and hydrolysis products. A blockwise repartition of the acetyl groups onto sugar beet pectin homogalacturonan is proposed.

Hydrogen production from sugar beet juice using an integrated biohydrogen process of dark fermentation and microbial electrolysis cell.

PubMed

Dhar, Bipro Ranjan; Elbeshbishy, Elsayed; Hafez, Hisham; Lee, Hyung-Sool

2015-12-01

An integrated dark fermentation and microbial electrochemical cell (MEC) process was evaluated for hydrogen production from sugar beet juice. Different substrate to inoculum (S/X) ratios were tested for dark fermentation, and the maximum hydrogen yield was 13% of initial COD at the S/X ratio of 2 and 4 for dark fermentation. Hydrogen yield was 12% of initial COD in the MEC using fermentation liquid end products as substrate, and butyrate only accumulated in the MEC. The overall hydrogen production from the integrated biohydrogen process was 25% of initial COD (equivalent to 6 mol H2/mol hexoseadded), and the energy recovery from sugar beet juice was 57% using the combined biohydrogen. Copyright © 2015 Elsevier Ltd. All rights reserved.

Evaluation of the fermentation of high gravity thick sugar beet juice worts for efficient bioethanol production

PubMed Central

2013-01-01

Background Sugar beet and intermediates of sugar beet processing are considered to be very attractive feedstock for ethanol production due to their content of fermentable sugars. In particular, the processing of the intermediates into ethanol is considerably facilitated because it does not require pretreatment or enzymatic treatment in contrast to production from starch raw materials. Moreover, the advantage of thick juice is high solid substance and saccharose content which eliminates problems with the storability of this feedstock. Results The objective of this study were to investigate bioethanol production from thick juice worts and the effects of their concentration, the type of mineral supplement, as well as the dose of yeast inoculum on fermentation dynamics and ethanol yield. The obtained results show that to ensure efficient ethanolic fermentation of high gravity thick juice worts, one needs to use a yeast strain with high ethanol tolerance and a large amount of inoculum. The highest ethanol yield (94.9 ± 2.8% of the theoretical yield) and sugars intake of 96.5 ± 2.9% were obtained after the fermentation of wort with an extract content of 250 g/kg supplemented with diammonium hydrogen phosphate (0.3 g/L of wort) and inoculated with 2 g of Ethanol Red dry yeast per L of wort. An increase in extract content in the fermentation medium from 250 g/L to 280 g/kg resulted in decreased efficiency of the process. Also the distillates originating from worts with an extract content of 250 g/kg were characterized by lower acetaldehyde concentration than those obtained from worts with an extract content of 280 g/kg. Conclusions Under the favorable conditions determined in our experiments, 38.9 ± 1.2 L of 100% (v/v) ethyl alcohol can be produced from 100 kg of thick juice. The obtained results show that the selection of process conditions and the yeast for the fermentation of worts with a higher sugar content can improve the economic performance of the

Airborne and ground-based remote sensing for the estimation of evapotranspiration and yield of bean, potato, and sugar beet crops

NASA Astrophysics Data System (ADS)

Jayanthi, Harikishan

The focus of this research was two-fold: (1) extend the reflectance-based crop coefficient approach to non-grain (potato and sugar beet), and vegetable crops (bean), and (2) develop vegetation index (VI)-yield statistical models for potato and sugar beet crops using high-resolution aerial multispectral imagery. Extensive crop biophysical sampling (leaf area index and aboveground dry biomass sampling) and canopy reflectance measurements formed the backbone of developing of canopy reflectance-based crop coefficients for bean, potato, and sugar beet crops in this study. Reflectance-based crop coefficient equations were developed for the study crops cultivated in Kimberly, Idaho, and subsequently used in water availability simulations in the plant root zone during 1998 and 1999 seasons. The simulated soil water profiles were compared with independent measurements of actual soil water profiles in the crop root zone in selected fields. It is concluded that the canopy reflectance-based crop coefficient technique can be successfully extended to non-grain crops as well. While the traditional basal crop coefficients generally expect uniform growth in a region the reflectance-based crop coefficients represent the actual crop growth pattern (in less than ideal water availability conditions) in individual fields. Literature on crop canopy interactions with sunlight states that there is a definite correspondence between leaf area index progression in the season and the final yield. In case of crops like potato and sugar beet, the yield is influenced not only on how early and how quickly the crop establishes its canopy but also on how long the plant stands on the ground in a healthy state. The integrated area under the crop growth curve has shown excellent correlations with hand-dug samples of potato and sugar beet crops in this research. Soil adjusted vegetation index-yield models were developed, and validated using multispectral aerial imagery. Estimated yield images were

Fungal secretomes enhance sugar beet pulp hydrolysis.

PubMed

Kracher, Daniel; Oros, Damir; Yao, Wanying; Preims, Marita; Rezic, Iva; Haltrich, Dietmar; Rezic, Tonci; Ludwig, Roland

2014-04-01

The recalcitrance of lignocellulose makes enzymatic hydrolysis of plant biomass for the production of second generation biofuels a major challenge. This work investigates an efficient and economic approach for the enzymatic hydrolysis of sugar beet pulp (SBP), which is a difficult to degrade, hemicellulose-rich by-product of the table sugar industry. Three fungal strains were grown on different substrates and the production of various extracellular hydrolytic and oxidative enzymes involved in pectin, hemicellulose, and cellulose breakdown were monitored. In a second step, the ability of the culture supernatants to hydrolyze thermally pretreated SBP was tested in batch experiments. The supernatant of Sclerotium rolfsii, a soil-borne facultative plant pathogen, was found to have the highest hydrolytic activity on SBP and was selected for further hydrolyzation experiments. A low enzyme load of 0.2 mg g(-1) protein from the culture supernatant was sufficient to hydrolyze a large fraction of the pectin and hemicelluloses present in SBP. The addition of Trichoderma reesei cellulase (1-17.5 mg g(-1) SBP) resulted in almost complete hydrolyzation of cellulose. It was found that the combination of pectinolytic, hemicellulolytic, and cellulolytic activities works synergistically on the complex SBP composite, and a combination of these hydrolytic enzymes is required to achieve a high degree of enzymatic SBP hydrolysis with a low enzyme load. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Carboxylate metabolism in sugar beet plants grown with excess Zn.

PubMed

Sagardoy, R; Morales, F; Rellán-Álvarez, R; Abadía, A; Abadía, J; López-Millán, A F

2011-05-01

The effects of Zn excess on carboxylate metabolism were investigated in sugar beet (Beta vulgaris L.) plants grown hydroponically in a growth chamber. Root extracts of plants grown with 50 or 100μM Zn in the nutrient solution showed increases in several enzymatic activities related to organic acid metabolism, including citrate synthase and phosphoenolpyruvate carboxylase, when compared to activities in control root extracts. Root citric and malic acid concentrations increased in plants grown with 100μM Zn, but not in plants grown with 50μM Zn. In the xylem sap, plants grown with 50 and 100μM Zn showed increases in the concentrations of citrate and malate compared to the controls. Leaves of plants grown with 50 or 100μM Zn showed increases in the concentrations of citric and malic acid and in the activities of citrate synthase and fumarase. Leaf isocitrate dehydrogenase increased only in plants grown with 50μM Zn when compared to the controls. In plants grown with 300μM Zn, the only enzyme showing activity increases in root extracts was citrate synthase, whereas the activities of other enzymes decreased compared to the controls, and root citrate concentrations increased. In the 300μM Zn-grown plants, the xylem concentrations of citric and malic acids were higher than those of controls, whereas in leaf extracts the activity of fumarase increased markedly, and the leaf citric acid concentration was higher than in the controls. Based on our data, a metabolic model of the carboxylate metabolism in sugar beet plants grown under Zn excess is proposed. Copyright © 2010 Elsevier GmbH. All rights reserved.

Sugar beet growth in a changing climate: past, present and future trends in southwest Germany

NASA Astrophysics Data System (ADS)

Kremer, Pascal; Fuchs, Hans-Joachim; Lang, Christian

2017-04-01

In the study, single factors and their impact on sugar beet cultivation against the background of past and projected climate change are being analyzed. The database consists of climate data by the German Weather Service and 1x1 km interpolated INTERMET raster data. Impact models were run to assess possible future trends using climate projection data of the REgional MOdel (REMO), emission scenario A1B, Run 1, data stream 2 for Germany, daily resolution, without bias correction, 10x10 km raster (n=150) (MPI on behalf of UBA 2006). Compared periods were: B:1971 2000; K:2021-2050; L:2071-2100. Agronomic data were collected from the field books of regional trials from 1974 2014 (n=448). Moreover, a business survey of regional farmers was carried out and evaluated. Impact models to predict timing for sowing, the date of field emergence and row closure, were derived from these data. The ontogenesis was simulated using a linear, temperature-based leaf-growth model. Sowing shifted forward by 7,3 days in regional field trials from 1974 2014. Progress-oriented, risk-tolerant farmers start sowing 10-14 days earlier compared to 1980. Recently, sowing is being conducted on average on 21 March in southwest Germany. For period K, 17 March, and for period L, 2 March is being projected as the average future sowing date while the same late frost risk applies compared to present climatic conditions. Shifting forward the sowing date with spring warming and, thus, exploiting the associated yield potential is the most promising agronomic adaptation strategy to the projected climate change on the farm level. In connection to earlier sowing, the field emergence tendentially shifted forward by 14 days in the field trials. Assuming sowing on 15 March, projection results show an advance of field emergence form 7 April in period B to 3 April in period L. Row closure in field trials in average shifted forward by 19,6 days. For period L, 29 May and thus, an earlier row closure of 9 days compared

Sugar beet polygalacturonase-inhibiting proteins with 11 LRRs confer Rhizoctonia, Fusarium and Botrytis resistance in Nicotiana plants

USDA-ARS?s Scientific Manuscript database

Polygalacturonase-inhibiting proteins (PGIPs) are leucine-rich repeat (LRR) proteins that inhibit polygalacturonase (PG) enzymes secreted by pathogens to break down plant cell walls during early stage of disease development. Sugar beet (Beta vulgaris L.) PGIP genes (BvPGIPs) have 11 LRR domains as ...

Evaluation of Rhizoctonia zeae as a potential biological control option for fungal root diseases of sugar beet

USDA-ARS?s Scientific Manuscript database

Several common root diseases routinely damage sugar beet in Nebraska and other production areas of the Central High Plains, and it is becoming more common to find fields infested simultaneously with multiple pathogens. Due to the lack of available chemicals for economic management of soilborne dise...

Efficient acetone-butanol-ethanol production by Clostridium beijerinckii from sugar beet pulp.

PubMed

Bellido, Carolina; Infante, Celia; Coca, Mónica; González-Benito, Gerardo; Lucas, Susana; García-Cubero, María Teresa

2015-08-01

Sugar beet pulp (SBP) has been investigated as a promising feedstock for ABE fermentation by Clostridium beijerinckii. Although lignin content in SBP is low, a pretreatment is needed to enhance enzymatic hydrolysis and fermentation yields. Autohydrolysis at pH 4 has been selected as the best pretreatment for SBP in terms of sugars release and acetone and butanol production. The best overall sugars release yields from raw SBP ranged from 66.2% to 70.6% for this pretreatment. The highest ABE yield achieved was 0.4g/g (5.1g/L of acetone and 6.6g/L butanol) and 143.2g ABE/kg SBP (62.3g acetone and 80.9g butanol) were obtained when pretreated SBP was enzymatically hydrolyzed at 7.5% (w/w) solid loading. Higher solid loadings (10%) offered higher acetone and butanol titers (5.8g/L of acetone and 7.8g/L butanol). All the experiments were carried out under not-controlling pH conditions reaching about 5.3 in the final samples. Copyright © 2015 Elsevier Ltd. All rights reserved.

A single U/C nucleotide substitution changing alanine to valine in the beet necrotic yellow vein virus P25 protein promotes increased virus accumulation in roots of mechanically inoculated, partially resistant sugar beet seedlings.

PubMed

Koenig, R; Loss, S; Specht, J; Varrelmann, M; Lüddecke, P; Deml, G

2009-03-01

Beet necrotic yellow vein virus (BNYVV) A type isolates E12 and S8, originating from areas where resistance-breaking had or had not been observed, respectively, served as starting material for studying the influence of sequence variations in BNYVV RNA 3 on virus accumulation in partially resistant sugar beet varieties. Sub-isolates containing only RNAs 1 and 2 were obtained by serial local lesion passages; biologically active cDNA clones were prepared for RNAs 3 which differed in their coding sequences for P25 aa 67, 68 and 129. Sugar beet seedlings were mechanically inoculated with RNA 1+2/RNA 3 pseudorecombinants. The origin of RNAs 1+2 had little influence on virus accumulation in rootlets. E12 RNA 3 coding for V(67)C(68)Y(129) P25, however, enabled a much higher virus accumulation than S8 RNA 3 coding for A(67)H(68)H(129) P25. Mutants revealed that this was due only to the V(67) 'GUU' codon as opposed to the A(67) 'GCU' codon.

Feruloylated and nonferuloylated arabino-oligosaccharides from sugar beet pectin selectively stimulate the growth of Bifidobacterium spp. in human fecal in vitro fermentations.

PubMed

Holck, Jesper; Lorentzen, Andrea; Vigsnæs, Louise K; Licht, Tine R; Mikkelsen, Jørn D; Meyer, Anne S

2011-06-22

The side chains of the rhamnogalacturonan I fraction in sugar beet pectin are particularly rich in arabinan moieties, which may be substituted with feruloyl groups. In this work the arabinan-rich fraction resulting from sugar beet pulp based pectin production was separated by Amberlite XAD hydrophobic interaction and membrane separation into four fractions based on feruloyl substitution and arabino-oligosaccharide chain length: short-chain (DP 2-10) and long-chain (DP 7-14) feruloylated and nonferuloylated arabino-oligosaccharides, respectively. HPAEC, SEC, and MALDI-TOF/TOF analyses of the fractions confirmed the presence of singly and doubly substituted feruloylated arabino-oligosaccharides in the feruloyl-substituted fractions. In vitro microbial fermentation by human fecal samples (n = 6 healthy human volunteers) showed a selective stimulation of bifidobacteria by both the feruloylated and the nonferuloylated long-chain arabino-oligosaccharides to the same extent as the prebiotic fructo-oligosaccharides control. None of the fractions stimulated the growth of the potential pathogen Clostridium difficile in monocultures. This work provides a first report on the separation of potentially bioactive feruloylated arabino-oligosaccharides from sugar beet pulp and an initial indication of the potentially larger bifidogenic effect of relatively long-chain arabino-oligosaccharides as opposed to short-chain arabino-oligosaccharides.

An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates.

PubMed

Cárdenas-Fernández, Max; Bawn, Maria; Hamley-Bennett, Charlotte; Bharat, Penumathsa K V; Subrizi, Fabiana; Suhaili, Nurashikin; Ward, David P; Bourdin, Sarah; Dalby, Paul A; Hailes, Helen C; Hewitson, Peter; Ignatova, Svetlana; Kontoravdi, Cleo; Leak, David J; Shah, Nilay; Sheppard, Tom D; Ward, John M; Lye, Gary J

2017-09-21

Over 8 million tonnes of sugar beet are grown annually in the UK. Sugar beet pulp (SBP) is the main by-product of sugar beet processing which is currently dried and sold as a low value animal feed. SBP is a rich source of carbohydrates, mainly in the form of cellulose and pectin, including d-glucose (Glu), l-arabinose (Ara) and d-galacturonic acid (GalAc). This work describes the technical feasibility of an integrated biorefinery concept for the fractionation of SBP and conversion of these monosaccharides into value-added products. SBP fractionation is initially carried out by steam explosion under mild conditions to yield soluble pectin and insoluble cellulose fractions. The cellulose is readily hydrolysed by cellulases to release Glu that can then be fermented by a commercial yeast strain to produce bioethanol at a high yield. The pectin fraction can be either fully hydrolysed, using physico-chemical methods, or selectively hydrolysed, using cloned arabinases and galacturonases, to yield Ara-rich and GalAc-rich streams. These monomers can be separated using either Centrifugal Partition Chromatography (CPC) or ultrafiltration into streams suitable for subsequent enzymatic upgrading. Building on our previous experience with transketolase (TK) and transaminase (TAm) enzymes, the conversion of Ara and GalAc into higher value products was explored. In particular the conversion of Ara into l-gluco-heptulose (GluHep), that has potential therapeutic applications in hypoglycaemia and cancer, using a mutant TK is described. Preliminary studies with TAm also suggest GluHep can be selectively aminated to the corresponding chiral aminopolyol. The current work is addressing the upgrading of the remaining SBP monomer, GalAc, and the modelling of the biorefinery concept to enable economic and Life Cycle Analysis (LCA).

Effects of zinc toxicity on sugar beet (Beta vulgaris L.) plants grown in hydroponics.

PubMed

Sagardoy, R; Morales, F; López-Millán, A-F; Abadía, A; Abadía, J

2009-05-01

The effects of high Zn concentration were investigated in sugar beet (Beta vulgaris L.) plants grown in a controlled environment in hydroponics. High concentrations of Zn sulphate in the nutrient solution (50, 100 and 300 microm) decreased root and shoot fresh and dry mass, and increased root/shoot ratios, when compared to control conditions (1.2 microm Zn). Plants grown with excess Zn had inward-rolled leaf edges and a damaged and brownish root system, with short lateral roots. High Zn decreased N, Mg, K and Mn concentrations in all plant parts, whereas P and Ca concentrations increased, but only in shoots. Leaves of plants treated with 50 and 100 microm Zn developed symptoms of Fe deficiency, including decreases in Fe, chlorophyll and carotenoid concentrations, increases in carotenoid/chlorophyll and chlorophyll a/b ratios and de-epoxidation of violaxanthin cycle pigments. Plants grown with 300 microm Zn had decreased photosystem II efficiency and further growth decreases but did not have leaf Fe deficiency symptoms. Leaf Zn concentrations of plants grown with excess Zn were high but fairly constant (230-260 microg.g(-1) dry weight), whereas total Zn uptake per plant decreased markedly with high Zn supply. These data indicate that sugar beet could be a good model to investigate Zn homeostasis mechanisms in plants, but is not an efficient species for Zn phytoremediation.

Phloem unloading in developing leaves of sugar beet

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmalstig, J.G.

1985-01-01

Physiological and transport data support a symplastic pathway for phloem unloading in developing leaves of sugar beet (Beta vulgaris L. Klein E, multigerm). The sulfhydryl inhibitor parachloromercuribenzene sulfonic acid (PCMBS) inhibited uptake of (/sup 14/C)-sucrose added to the free space of developing leaves, but did not affect import of (/sup 14/C)-sucrose during steady-state /sup 14/CO/sub 2/ labeling of a source leaf. The passively-transported xenobiotic sugar, (/sup 14/C)-L-glucose did not readily enter mesophyll cells when supplied through the cut end of the petiole of a sink leaf as determined by whole leaf autoradiography. In contrast, (/sup 14/C)-L-glucose translocated through the phloemmore » from a mature leaf, rapidly entered mesophyll cells, and was evenly distributed between mesophyll and veins. Autoradiographs of developing leaves following a pulse of /sup 14/CO/sub 2/ to a source leaf revealed rapid passage of phloem translocated into progressively higher order veins as the leaf developed. Entry into V order veins occurred during the last stage of import through the phloem. Import into developing leaves was inhibited by glyphosate (N-phosphomethylglycine), a herbicide which inhibits the aromatic amino acid pathway and hence protein synthesis. Glyphosate also stopped net starch accumulation in sprayed mature leaves, but did not affect export of carbon from treated leaves during the time period that import into developed leaves was inhibited.« less

Biological and molecular characterization of Beet oak-leaf virus

USDA-ARS?s Scientific Manuscript database

Beet oak-leaf virus (BOLV) was first isolated from Rhizomania infested fields in California in early 2000. The infected sugar beet leaves showed oak-leaf pattern symptoms in some breeding lines different from Rhizomania, while some beet cultivars were symptomless. BOLV is transmitted by Polymyxe bet...

Comparing salt tolerance of beet cultivars and their halophytic ancestor: consequences of domestication and breeding programmes

PubMed Central

Rozema, Jelte; Cornelisse, Danny; Zhang, Yuancheng; Li, Hongxiu; Bruning, Bas; Katschnig, Diana; Broekman, Rob; Ji, Bin; van Bodegom, Peter

2015-01-01

Salt tolerance of higher plants is determined by a complex set of traits, the timing and rate of evolution of which are largely unknown. We compared the salt tolerance of cultivars of sugar beet and their ancestor, sea beet, in hydroponic studies and evaluated whether traditional domestication and more recent breeding have changed salt tolerance of the cultivars relative to their ancestor. Our comparison of salt tolerance of crop cultivars is based on values of the relative growth rate (RGR) of the entire plant at various salinity levels. We found considerable salt tolerance of the sea beet and slightly, but significantly, reduced salt tolerance of the sugar beet cultivars. This indicates that traditional domestication by selection for morphological traits such as leaf size, beet shape and size, enhanced productivity, sugar content and palatability slightly affected salt tolerance of sugar beet cultivars. Salt tolerance among four sugar beet cultivars, three of which have been claimed to be salt tolerant, did not differ. We analysed the components of RGR to understand the mechanism of salt tolerance at the whole-plant level. The growth rate reduction at higher salinity was linked with reduced leaf area at the whole-plant level (leaf area ratio) and at the individual leaf level (specific leaf area). The leaf weight fraction was not affected by increased salinity. On the other hand, succulence and leaf thickness and the net assimilation per unit of leaf area (unit leaf rate) increased in response to salt treatment, thus partially counteracting reduced capture of light by lower leaf area. This compensatory mechanism may form part of the salt tolerance mechanism of sea beet and the four studied sugar beet cultivars. Together, our results indicate that domestication of the halophytic ancestor sea beet slightly reduced salt tolerance and that breeding for improved salt tolerance of sugar beet cultivars has not been effective. PMID:25492122

Osmotic Stress Induces Expression of Choline Monooxygenase in Sugar Beet and Amaranth1

PubMed Central

Russell, Brenda L.; Rathinasabapathi, Bala; Hanson, Andrew D.

1998-01-01

Choline monooxygenase (CMO) catalyzes the committing step in the synthesis of glycine betaine, an osmoprotectant accumulated by many plants in response to salinity and drought. To investigate how these stresses affect CMO expression, a spinach (Spinacia oleracea L., Chenopodiaceae) probe was used to isolate CMO cDNAs from sugar beet (Beta vulgaris L., Chenopodiaceae), a salt- and drought-tolerant crop. The deduced beet CMO amino acid sequence comprised a transit peptide and a 381-residue mature peptide that was 84% identical (97% similar) to that of spinach and that showed the same consensus motif for coordinating a Rieske-type [2Fe-2S] cluster. A mononuclear Fe-binding motif was also present. When water was withheld, leaf relative water content declined to 59% and the levels of CMO mRNA, protein, and enzyme activity rose 3- to 5-fold; rewatering reversed these changes. After gradual salinization (NaCl:CaCl2 = 5.7:1, mol/mol), CMO mRNA, protein, and enzyme levels in leaves increased 3- to 7-fold at 400 mm salt, and returned to uninduced levels when salt was removed. Beet roots also expressed CMO, most strongly when salinized. Salt-inducible CMO mRNA, protein, and enzyme activity were readily detected in leaves of Amaranthus caudatus L. (Amaranthaceae). These data show that CMO most probably has a mononuclear Fe center, is inducibly expressed in roots as well as in leaves of Chenopodiaceae, and is not unique to this family. PMID:9489025

Energy beets: an undiscovered crop for the Southeastern US

USDA-ARS?s Scientific Manuscript database

Energy beets (Beta vulgaris), which are sugar beets grown for non-food sources, are a potential winter cash crop for growers in the southeastern U.S. that are planted in the autumn and harvested in the spring, complementing current summer crop rotations. The end-product from energy beets will be in...

Assessment of soil quality index for wheat and sugar beet cropping systems on an entisol in Central Anatolia.

PubMed

Şeker, Cevdet; Özaytekin, Hasan Hüseyin; Negiş, Hamza; Gümüş, İlknur; Dedeoğlu, Mert; Atmaca, Emel; Karaca, Ümmühan

2017-04-01

The sustainable use of agricultural lands is significantly affected by the implemented management and land processing methods. In sugar beet and wheat cropping, because the agronomic characteristics of plants are different, the tillage methods applied also exhibit significant variability. Soil quality concept is used, as a holistic approach to determining the effects of these applications on the sustainable use of soil. Agricultural soil quality evaluation is essential for economic success and environmental stability in rapidly developing regions. At present, a variety of methods are used to evaluate soil quality using different indicators. This study was conducted in one of the most important irrigated agriculture areas of Çumra plain in Central Anatolia, Turkey. In the soil under sugar beet and wheat cultivation, 12 soil quality indicators (aggregate stability (AS), available water capacity (AWC), surface penetration resistance (PR 0-20 ), subsurface penetration resistance (PR 20-40 ), organic matter (OM), active carbon (AC), potentially mineralizable nitrogen (PMN), root health value (RHV), pH, available phosphorus (AP), potassium (K), and macro-micro elements (ME) (Mg, Fe, Mn, and Zn)) were measured and scored according to the Cornell Soil Health Assessment (CSHA) and the Soil Management Assessment Framework (SMAF). The differences among 8 (AS, AWC, PR 0-20 , PR 20-40 , AC, PMN, AP, and ME) of these 12 soil quality characteristics measured in two different plant cultivation were found statistically significant. The result of the soil quality evaluation with scoring function in the examined area revealed a soil quality score of 61.46 in the wheat area and of 51.20 in the sugar beet area, which can be classified as medium and low, respectively. Low soil quality scores especially depend on physical and biological soil properties. Therefore, improvement of soil physical and biological properties with sustainable management is necessary to enhance the soil quality

Aggregation Behavior and a Putative Aggregation Pheromone in Sugar Beet Root Maggot Flies (Diptera: Ulidiidae)

PubMed Central

Emmert, Susan Y.; Tindall, Kelly; Ding, Hongjian; Boetel, Mark A.; Rajabaskar, D.; Eigenbrode, Sanford D.

2017-01-01

Male-biased aggregations of sugar beet root maggot, Tetanops myopaeformis (Röder) (Diptera: Ulidiidae), flies were observed on utility poles near sugar beet (Beta vulgaris L. [Chenopodiaceae]) fields in southern Idaho; this contrasts with the approximately equal sex ratio typically observed within fields. Peak observation of mating pairs coincided with peak diurnal abundance of flies. Volatiles released by individual male and female flies were sampled from 08:00 to 24:00 hours in the laboratory using solid-phase microextraction and analyzed using gas chromatography/mass spectrometry (GC/MS). Eleven compounds were uniquely detected from males. Three of these compounds (2-undecanol, 2-decanol, and sec-nonyl acetate) were detected in greater quantities during 12:00–24:00 hours than during 08:00–12:00 hours. The remaining eight compounds uniquely detected from males did not exhibit temporal trends in release. Both sexes produced 2-nonanol, but males produced substantially higher (ca. 80-fold) concentrations of this compound than females, again peaking after 12:00 hours. The temporal synchrony among male aggregation behavior, peak mating rates, and release of certain volatile compounds by males suggest that T. myopaeformis flies exhibit lekking behavior and produce an associated pheromone. Field assays using synthetic blends of the putative aggregation pheromone showed evidence of attraction in both females and males. PMID:28423428

Process model and economic analysis of ethanol production from sugar beet raw juice as part of the cleaner production concept.

PubMed

Vučurović, Damjan G; Dodić, Siniša N; Popov, Stevan D; Dodić, Jelena M; Grahovac, Jovana A

2012-01-01

The batch fermentation process of sugar beet processing intermediates by free yeast cells is the most widely used method in the Autonomous Province of Vojvodina for producing ethanol as fuel. In this study a process and cost model was developed for producing ethanol from raw juice. The model can be used to calculate capital investment costs, unit production costs and operating costs for a plant producing 44 million l of 99.6% pure ethanol annually. In the sensitivity analysis the influence of sugar beet and yeast price, as well as the influence of recycled biomass on process economics, ethanol production costs and project feasibility was examined. The results of this study clearly demonstrate that the raw material costs have a significant influence on the expenses for producing ethanol. Also, the optimal percentage of recycled biomass turned out to be in the range from 50% to 70%. Copyright © 2011 Elsevier Ltd. All rights reserved.

Influence of lignocellulose and low or high levels of sugar beet pulp on nutrient digestibility and the fecal microbiota in dogs.

PubMed

Kröger, S; Vahjen, W; Zentek, J

2017-04-01

Lignocellulose is an alternative fiber source for dogs; however, it has not yet been studied as a feed ingredient for the nutrition of dogs. Eight adult Beagles were involved in the study, which consisted of 3 feeding periods of 8 to 12 wk each. All dogs received 3 different diets, which either had the same concentration of fiber sources (2.7% sugar beet pulp or lignocellulose) or were formulated for a similar concentration of approximately 3% crude fiber: 12% sugar beet pulp (highSBP; 3.1% crude fiber), 2.7% sugar beet pulp (lowSBP; 0.96% crude fiber), or 2.7% lignocellulose (LC; 2.4% crude fiber). Feces samples were collected at the end of each feeding period, and the apparent nutrient digestibility, daily amount, and DM content of feces and fecal cell numbers of relevant bacteria were analyzed. The daily feces amount was lower and the feces DM was higher when dogs were fed the LC diet and the lowSBP diet compared with the highSBP diet ( < 0.001). Apparent digestibility of CP, Na, and K was highest with the lowSBP diet followed by the LC and highSBP diets ( < 0.001). After feeding LC, the bacterial cell counts of spp., spp., and the cluster were reduced compared with feeding highSBP and even more reduced after feeding lowSBP ( < 0.001). The bacterial cell count of the cluster was lower in LC and lowSBP compared with highSBP ( < 0.001). The feces of dogs fed LC and lowSBP had lower concentrations of acetate ( < 0.001), propionate ( < 0.001), -butyrate ( = 0.015), total fatty acids ( < 0.001), and lactate ( < 0.001) compared with dogs fed highSBP. The concentration of -butyrate was higher in the feces of dogs fed with LC compared with dogs fed high and low sugar beet pulp (SBP; < 0.001). The pH of the feces of the LC-fed dogs was highest followed by lowSBP- and highSBP-fed dogs ( < 0.001). Depending on the concentration, the use of LC and SBP as fiber sources in dog feed has different impacts on the fecal microbiota and the apparent digestibility of nutrients

Belowground plant development measured with magnetic resonance imaging (MRI): exploiting the potential for non-invasive trait quantification using sugar beet as a proxy

PubMed Central

Metzner, Ralf; van Dusschoten, Dagmar; Bühler, Jonas; Schurr, Ulrich; Jahnke, Siegfried

2014-01-01

Both structural and functional properties of belowground plant organs are critical for the development and yield of plants but, compared to the shoot, much more difficult to observe due to soil opacity. Many processes concerning the belowground plant performance are not fully understood, in particular spatial and temporal dynamics and their interrelation with environmental factors. We used Magnetic Resonance Imaging (MRI) as a noninvasive method to evaluate which traits can be measured when a complex plant organ is monitored in-vivo while growing in the soil. We chose sugar beet (Beta vulgaris ssp. vulgaris) as a model system. The beet consists mainly of root tissues, is rather complex regarding tissue structure and responses to environmental factors, and thereby a good object to test the applicability of MRI for 3D phenotyping approaches. Over a time period of up to 3 months, traits such as beet morphology or anatomy were followed in the soil and the effect of differently sized pots on beet fresh weight calculated from MRI data was studied. There was a clear positive correlation between the pot size and the increase in fresh weight of a sugar beet over time. Since knowledge of the development of internal beet structures with several concentric cambia, vascular and parenchyma rings is still limited, we consecutively acquired 3D volumetric images on individual plants using the MRI contrast parameter T2 to map the development of rings at the tissue level. This demonstrates that MRI provides versatile protocols to non-invasively measure plant traits in the soil. It opens new avenues to investigate belowground plant performance under adverse environmental conditions such as drought, nutrient shortage, or soil compaction to seek for traits of belowground organs making plants more resilient to stress. PMID:25278947

Assessment of spore presence for Cercospora beticola as demonstrated by sentinel beets

USDA-ARS?s Scientific Manuscript database

Cercospora beticola, the causal agent of Cercospora leaf spot (CLS) in Beta vulgaris (sugar, table, and leaf beet), is an important pathogen globally. Disease forecasting models are widely used to aid in CLS management for sugar beet. Most models rely on weather data to predict infection periods but...

"We Were Beet Workers, and that Was All": Beet Field Laborers in the North Platte Valley, 1902-1930

ERIC Educational Resources Information Center

Kipp, Dustin

2011-01-01

The experiences of the men, women, and children who labored in the beet fields of the North Platte Valley changed significantly as the sugar beet industry went through a period of rapid expansion prior to 1920 and then reached a relatively stable plateau. During the period of expansion, laborers were attracted by promises of reasonable wages, good…

The hrpZ Gene of Pseudomonas syringae pv. phaseolicola Enhances Resistance to Rhizomania Disease in Transgenic Nicotiana benthamiana and Sugar Beet

PubMed Central

Pavli, Ourania I.; Kelaidi, Georgia I.; Tampakaki, Anastasia P.; Skaracis, George N.

2011-01-01

To explore possible sources of transgenic resistance to the rhizomania-causing Beet necrotic yellow vein virus (BNYVV), Nicotiana benthamiana plants were constructed to express the harpin of Pseudomonas syringae pv. phaseolicola (HrpZPsph). The HrpZ protein was expressed as an N-terminal fusion to the PR1 signal peptide (SP/HrpZ) to direct harpin accumulation to the plant apoplast. Transgene integration was verified by mPCR in all primary transformants (T0), while immunoblot analysis confirmed that the protein HrpZPsph was produced and the signal peptide was properly processed. Neither T0 plants nor selfed progeny (T1) showed macroscopically visible necrosis or any other macroscopic phenotypes. However, plants expressing the SP/HrpZPsph showed increased vigor and grew faster in comparison with non-transgenic control plants. Transgenic resistance was assessed after challenge inoculation with BNYVV on T1 progeny by scoring of disease symptoms and by DAS-ELISA at 20 and 30 dpi. Transgenic and control lines showed significant differences in terms of the number of plants that became infected, the timing of infection and the disease symptoms displayed. Plants expressing the SP/HrpZPsph developed localized leaf necrosis in the infection area and had enhanced resistance upon challenge with BNYVV. In order to evaluate the SP/HrpZ-based resistance in the sugar beet host, A. rhizogenes-mediated root transformation was exploited as a transgene expression platform. Upon BNYVV inoculation, transgenic sugar beet hairy roots showed high level of BNYVV resistance. In contrast, the aerial non-transgenic parts of the same seedlings had virus titers that were comparable to those of the seedlings that were untransformed or transformed with wild type R1000 cells. These findings indicate that the transgenically expressed SP/HrpZ protein results in enhanced rhizomania resistance both in a model plant and sugar beet, the natural host of BNYVV. Possible molecular mechanisms underlying the

The hrpZ gene of Pseudomonas syringae pv. phaseolicola enhances resistance to rhizomania disease in transgenic Nicotiana benthamiana and sugar beet.

PubMed

Pavli, Ourania I; Kelaidi, Georgia I; Tampakaki, Anastasia P; Skaracis, George N

2011-03-04

To explore possible sources of transgenic resistance to the rhizomania-causing Beet necrotic yellow vein virus (BNYVV), Nicotiana benthamiana plants were constructed to express the harpin of Pseudomonas syringae pv. phaseolicola (HrpZ(Psph)). The HrpZ protein was expressed as an N-terminal fusion to the PR1 signal peptide (SP/HrpZ) to direct harpin accumulation to the plant apoplast. Transgene integration was verified by mPCR in all primary transformants (T0), while immunoblot analysis confirmed that the protein HrpZ(Psph) was produced and the signal peptide was properly processed. Neither T0 plants nor selfed progeny (T1) showed macroscopically visible necrosis or any other macroscopic phenotypes. However, plants expressing the SP/HrpZ(Psph) showed increased vigor and grew faster in comparison with non-transgenic control plants. Transgenic resistance was assessed after challenge inoculation with BNYVV on T1 progeny by scoring of disease symptoms and by DAS-ELISA at 20 and 30 dpi. Transgenic and control lines showed significant differences in terms of the number of plants that became infected, the timing of infection and the disease symptoms displayed. Plants expressing the SP/HrpZ(Psph) developed localized leaf necrosis in the infection area and had enhanced resistance upon challenge with BNYVV. In order to evaluate the SP/HrpZ-based resistance in the sugar beet host, A. rhizogenes-mediated root transformation was exploited as a transgene expression platform. Upon BNYVV inoculation, transgenic sugar beet hairy roots showed high level of BNYVV resistance. In contrast, the aerial non-transgenic parts of the same seedlings had virus titers that were comparable to those of the seedlings that were untransformed or transformed with wild type R1000 cells. These findings indicate that the transgenically expressed SP/HrpZ protein results in enhanced rhizomania resistance both in a model plant and sugar beet, the natural host of BNYVV. Possible molecular mechanisms

Pretreatment of Sugar Beet Pulp with Dilute Sulfurous Acid is Effective for Multipurpose Usage of Carbohydrates.

PubMed

Kharina, M; Emelyanov, V; Mokshina, N; Ibragimova, N; Gorshkova, T

2016-05-01

Sulfurous acid was used for pretreatment of sugar beet pulp (SBP) in order to achieve high efficiency of both extraction of carbohydrates and subsequent enzymatic hydrolysis of the remaining solids. The main advantage of sulfurous acid usage as pretreatment agent is the possibility of its regeneration. Application of sulfurous acid as hydrolyzing agent in relatively low concentrations (0.6-1.0 %) during a short period of time (10-20 min) and low solid to liquid ratio (1:3, 1:6) allowed effective extraction of carbohydrates from SBP and provided positive effect on subsequent enzymatic hydrolysis. The highest obtained concentration of reducing substances (RS) in hydrolysates was 8.5 %; up to 33.6 % of all carbohydrates present in SBP could be extracted. The major obtained monosaccharides were arabinose and glucose (9.4 and 7.3 g/l, respectively). Pretreatment of SBP with sulfurous acid increased 4.6 times the yield of glucose during subsequent enzymatic hydrolysis of remaining solids with cellulase cocktail, as compared to the untreated SBP. Total yield of glucose during SBP pretreatment and subsequent enzymatic hydrolysis amounted to 89.4 % of the theoretical yield. The approach can be applied directly to the wet SBP. Hydrolysis of sugar beet pulp with sulfurous acid is recommended for obtaining of individual monosaccharides, as well as nutritional media.

Beet curly top resistance in USDA-ARS plant introduction lines, 2016

USDA-ARS?s Scientific Manuscript database

Curly top caused by Beet curly top virus (BCTV) is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. In order to iden...

Beet curly top resistance in USDA-ARS Kimberly germplasm lines, 2015

USDA-ARS?s Scientific Manuscript database

Curly top caused by Beet curly top virus is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. In order to identify no...

Beet curly top resistance in USDA-ARS Ft. Collins germplasm, 2017

USDA-ARS?s Scientific Manuscript database

Curly top caused by Beet curly top virus (BCTV) is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. In order to iden...

Beet curly top resistance in USDA-ARS plant introduction lines, 2017

USDA-ARS?s Scientific Manuscript database

Curly top caused by Beet curly top virus (BCTV) is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. In order to iden...

Phylogenetic relationships and the occurrence of interspecific recombination between beet chlorosis virus (BChV) and Beet mild yellowing virus (BMYV).

PubMed

Kozlowska-Makulska, Anna; Hasiow-Jaroszewska, Beata; Szyndel, Marek S; Herrbach, Etienne; Bouzoubaa, Salah; Lemaire, Olivier; Beuve, Monique

2015-02-01

Samples containing two viruses belonging to the genus Polerovirus, beet chlorosis virus (BChV) and beet mild yellowing virus (BMYV), were collected from French and Polish sugar beet fields. The molecular properties of 24 isolates of BChV and BMYV were investigated, and their genetic diversity was examined in the coat protein (CP)- and P0-encoding genes. For the first time, we have demonstrated that beet polerovirus populations include recombinants between BChV and BMYV containing breakpoints within the CP gene. Moreover, a partial correlation between geographic origin and phylogenetic clustering was observed for BMYV isolates.

Quaternary IPM (integrated pest management)--concept for the control of powdery mildew in sugar beets.

PubMed

Wolf, P F J; Verreet, A

2008-01-01

Powdery mildew, caused by Erysiphe betae (Vanha) Weltzien, may be assumed as an important leaf disease in sugar beet growing areas of central Europe. Although the causal agent is mainly adapted to arid climatic zones, the disease is appearing every year, where the extent of infection is mainly dependent on weather conditions and susceptibility of cultivar. The losses caused by powdery mildew seldom exceed 10% of sugar yield; moreover, losses are likely only under the condition that the epidemic onset occurs before end-August. Nevertheless, the epidemic onset varies in a wide range, as there are years with high incidence followed by growing periods without severe infection. Therefore, in order to have a flexible control of the disease, where the use of fungicides could be minimised to an essential amount, a quaternary IPM (Integrated Pest Management) -concept was developed. The development is based on epidemiological field studies (Germany, 1993-2004, n = 76) of sugar beet leaf diseases under variation of year, site and cultivar. Efficacy of fungicide treatment timing was assessed in relation to the epidemic development. Comparison of treatments comprised fungicide sprays carried out from disease initiation till later stages of the epidemic. Additionally, the assessments were performed in relation to an untreated and a healthy control--the latter was three times treated according to a treatment regime with three to four week intervals. The effect of different application timings was measured by the potential of disease and yield loss control. The quaternary concept combines the advantages of four elements in order to compensate the constraints of the single tools: The period without disease risk is determined by a so-called negative-prognosis (i). First symptoms appear in the period from mid-July till the beginning of September. If disease initiation cannot be excluded, field observations by a sample of 100 leaves are advised. The disease scores enable the appliance

Evaluation of the fermentation of high gravity thick sugar beet juice worts for efficient bioethanol production.

PubMed

Dziugan, Piotr; Balcerek, Maria; Pielech-Przybylska, Katarzyna; Patelski, Piotr

2013-11-08

Sugar beet and intermediates of sugar beet processing are considered to be very attractive feedstock for ethanol production due to their content of fermentable sugars. In particular, the processing of the intermediates into ethanol is considerably facilitated because it does not require pretreatment or enzymatic treatment in contrast to production from starch raw materials. Moreover, the advantage of thick juice is high solid substance and saccharose content which eliminates problems with the storability of this feedstock. The objective of this study were to investigate bioethanol production from thick juice worts and the effects of their concentration, the type of mineral supplement, as well as the dose of yeast inoculum on fermentation dynamics and ethanol yield.The obtained results show that to ensure efficient ethanolic fermentation of high gravity thick juice worts, one needs to use a yeast strain with high ethanol tolerance and a large amount of inoculum. The highest ethanol yield (94.9 ± 2.8% of the theoretical yield) and sugars intake of 96.5 ± 2.9% were obtained after the fermentation of wort with an extract content of 250 g/kg supplemented with diammonium hydrogen phosphate (0.3 g/L of wort) and inoculated with 2 g of Ethanol Red dry yeast per L of wort. An increase in extract content in the fermentation medium from 250 g/L to 280 g/kg resulted in decreased efficiency of the process. Also the distillates originating from worts with an extract content of 250 g/kg were characterized by lower acetaldehyde concentration than those obtained from worts with an extract content of 280 g/kg. Under the favorable conditions determined in our experiments, 38.9 ± 1.2 L of 100% (v/v) ethyl alcohol can be produced from 100 kg of thick juice. The obtained results show that the selection of process conditions and the yeast for the fermentation of worts with a higher sugar content can improve the economic performance of the alcohol-distilling industry due to

Salt-induction of betaine aldehyde dehydrogenase mRNA, protein, and enzymatic activity in sugar beet. [Beta vulgaris L

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCue, K.F.; Hanson, A.D.

1991-05-01

In Chenopodiaceae such as sugar beet (Beta vulgaris L.), glycine betaine (betaine) accumulates in response to drought or salinity stress and functions in the cytoplasm as a compatible osmolyte. The last enzyme in the biosynthetic pathway, betaine aldehyde dehydrogenase (BADH), increases as much as 4-fold in response to rising salinity in the external medium. This increase is accompanied by an increase in both protein and mRNA levels. The steady state increases in BADH were examined at a series of NaCl concentrations from 100 to 500 mM NaCl. BADH protein levels were examined by native PAGE, and by western blot analysismore » using antibodies raised against BADH purified from spinach. mRNA levels were examined by northern plot analysis of total RNA isolated from the leaves and hybridized with a sugar beet BADH cDNA clone. The time course for BADH mRNA induction was determined in a salt shock experiment utilizing 400 mM NaCl added to the external growth medium. Disappearance of BADH was examined in a salt relief experiment using plants step-wise salinized to 500 mM NaCl and then returned to 0 mM NaCl.« less

Changes in physical, chemical and functional properties of whey protein isolate (WPI) and sugar beet pectin (SBP) conjugates formed by controlled dry-heating

USDA-ARS?s Scientific Manuscript database

A Maillard type reaction in the dry state was utilized to create conjugates between whey protein isolate (WPI) and sugar beet pectin (SBP) to achieve improved functional properties including solubility, colloidal stability and oil-in-water emulsion stability. To optimize the reaction conditions, mi...

New Generation of Resistant Sugar Beet Varieties for Advanced Integrated Management of Cercospora Leaf Spot in Central Europe.

PubMed

Vogel, Johannes; Kenter, Christine; Holst, Carsten; Märländer, Bernward

2018-01-01

Cercospora leaf spot (CLS) epidemics in sugar beet have been increasing in recent years causing higher use of fungicides. Concomitantly, the availability of effective fungicides is at risk because of resistance development in the fungus, the lack of new active ingredients as well as restrictive approval practices. A key option for an integrated management of CLS is cultivation of resistant varieties. Because of the yield penalty in resistant varieties, acceptance in commercial practice so far has been low. The aim of our study was to characterize recent sugar beet varieties registered in Germany in terms of resistance and tolerance to CLS and their value for integrated pest management. The genetic basis of CLS resistance in varieties is protected by intellectual property rights even after variety registration and not open to the public due to economic competition. To gain reliable data for cultivation, varieties have to be tested for their resistance traits under field conditions at varying levels of infection with Cercospora beticola . In collaboration with variety related stakeholders, 15 sugar beet varieties were tested in 49 field trials in Germany from 2014 to 2016 for their yield response to CLS. The trials were set up in a split-plot design with and without infection (i.e., with and without fungicide). The classification of varietal reaction to CLS is based on symptomatic leaf area (susceptibility) and the resulting relative yield loss (tolerance). Since the relation between both parameters varied among varieties, it was used as an additional parameter to describe tolerance. On this basis, three groups of varieties were identified. They can be characterized as a susceptible, a resistant and a presumably tolerant cluster. A comparison of the data with an older dataset originating from 2009 to 2011 revealed that yield performance of recent varieties with resistance to C. beticola caught up with susceptible varieties due to breeding progress. They showed no

New Generation of Resistant Sugar Beet Varieties for Advanced Integrated Management of Cercospora Leaf Spot in Central Europe

PubMed Central

Vogel, Johannes; Kenter, Christine; Holst, Carsten; Märländer, Bernward

2018-01-01

Cercospora leaf spot (CLS) epidemics in sugar beet have been increasing in recent years causing higher use of fungicides. Concomitantly, the availability of effective fungicides is at risk because of resistance development in the fungus, the lack of new active ingredients as well as restrictive approval practices. A key option for an integrated management of CLS is cultivation of resistant varieties. Because of the yield penalty in resistant varieties, acceptance in commercial practice so far has been low. The aim of our study was to characterize recent sugar beet varieties registered in Germany in terms of resistance and tolerance to CLS and their value for integrated pest management. The genetic basis of CLS resistance in varieties is protected by intellectual property rights even after variety registration and not open to the public due to economic competition. To gain reliable data for cultivation, varieties have to be tested for their resistance traits under field conditions at varying levels of infection with Cercospora beticola. In collaboration with variety related stakeholders, 15 sugar beet varieties were tested in 49 field trials in Germany from 2014 to 2016 for their yield response to CLS. The trials were set up in a split-plot design with and without infection (i.e., with and without fungicide). The classification of varietal reaction to CLS is based on symptomatic leaf area (susceptibility) and the resulting relative yield loss (tolerance). Since the relation between both parameters varied among varieties, it was used as an additional parameter to describe tolerance. On this basis, three groups of varieties were identified. They can be characterized as a susceptible, a resistant and a presumably tolerant cluster. A comparison of the data with an older dataset originating from 2009 to 2011 revealed that yield performance of recent varieties with resistance to C. beticola caught up with susceptible varieties due to breeding progress. They showed no

Effect of Different Sugar Beet Pulp Pretreatments on Biogas Production Efficiency.

PubMed

Ziemiński, Krzysztof; Kowalska-Wentel, Monika

2017-03-01

The objective of this study was to determine the effect of different sugar beet pulp (SBP) pretreatments on biogas yield from anaerobic digestion. SBP was subjected to grinding, thermal-pressure processing, enzymatic hydrolysis, or combination of these pretreatments. It was observed that grinding of SBP to 2.5-mm particles resulted in the cumulative biogas productivity of 617.2 mL/g volatile solids (VS), which was 20.2 % higher compared to the biogas yield from the not pretreated SBP, and comparable to that from not ground, enzymatically hydrolyzed SBP. The highest cumulative biogas productivity, 898.7 mL/g VS, was obtained from the ground, thermal-pressure pretreated and enzymatically hydrolyzed SBP. The latter pretreatment variant enabled to achieve the highest glucose concentration (24.765 mg/mL) in the enzymatic hydrolysates. The analysis of energy balance showed that the increase in the number of SBP pretreatment operations significantly reduced the gain of electric energy.

Heterologous expression, purification and characterization of three novel esterases secreted by the lignocellulolytic fungus Penicillium purpurogenum when grown on sugar beet pulp

PubMed Central

Oleas, Gabriela; Callegari, Eduardo; Sepúlveda, Romina; Eyzaguirre, Jaime

2017-01-01

The lignocellulolytic fungus, Penicillium purpurogenum, grows on a variety of natural carbon sources, among them sugar beet pulp. Culture supernatants of P. purpurogenum grown on sugar beet pulp were partially purified and the fractions obtained analyzed for esterase activity by zymograms. The bands with activity on methyl umbelliferyl acetate were subjected to mass spectrometry to identify peptides. The peptides obtained were probed against the proteins deduced from the genome sequence of P. purpurogenum. Eight putative esterases thus identified were chosen for future work. Their cDNAs were expressed in Pichia pastoris. The supernatants of the recombinant clones were assayed for esterase activity, and five of the proteins were active against one or more substrates: methyl umbelliferyl acetate, indoxyl acetate, methyl esterified pectin and fluorescein diacetate. Three of those enzymes were purified, further characterized and subjected to a BLAST search. Based on their amino acid sequence and properties, they were identified as follows: RAE1, pectin acetyl esterase (CAZy family CE 12); FAEA, feruloyl esterase (could not be assigned to a CAZy family) and EAN, acetyl esterase (former CAZy family CE 10). PMID:28342968

Centrifugal partition chromatography in a biorefinery context: Separation of monosaccharides from hydrolysed sugar beet pulp.

PubMed

Ward, David P; Cárdenas-Fernández, Max; Hewitson, Peter; Ignatova, Svetlana; Lye, Gary J

2015-09-11

A critical step in the bioprocessing of sustainable biomass feedstocks, such as sugar beet pulp (SBP), is the isolation of the component sugars from the hydrolysed polysaccharides. This facilitates their subsequent conversion into higher value chemicals and pharmaceutical intermediates. Separation methodologies such as centrifugal partition chromatography (CPC) offer an alternative to traditional resin-based chromatographic techniques for multicomponent sugar separations. Highly polar two-phase systems containing ethanol and aqueous ammonium sulphate are examined here for the separation of monosaccharides present in hydrolysed SBP pectin: l-rhamnose, l-arabinose, d-galactose and d-galacturonic acid. Dimethyl sulfoxide (DMSO) was selected as an effective phase system modifier improving monosaccharide separation. The best phase system identified was ethanol:DMSO:aqueous ammonium sulphate (300gL(-1)) (0.8:0.1:1.8, v:v:v) which enabled separation of the SBP monosaccharides by CPC (200mL column) in ascending mode (upper phase as mobile phase) with a mobile phase flow rate of 8mLmin(-1). A mixture containing all four monosaccharides (1.08g total sugars) in the proportions found in hydrolysed SBP was separated into three main fractions; a pure l-rhamnose fraction (>90%), a mixed l-arabinose/d-galactose fraction and a pure d-galacturonic acid fraction (>90%). The separation took less than 2h demonstrating that CPC is a promising technique for the separation of these sugars with potential for application within an integrated, whole crop biorefinery. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

29 CFR 780.819 - Production must be of unrefined sugar or syrup.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false Production must be of unrefined sugar or syrup. 780.819... STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15...

29 CFR 780.819 - Production must be of unrefined sugar or syrup.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false Production must be of unrefined sugar or syrup. 780.819... STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15...

29 CFR 780.819 - Production must be of unrefined sugar or syrup.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false Production must be of unrefined sugar or syrup. 780.819... STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15...

29 CFR 780.819 - Production must be of unrefined sugar or syrup.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Production must be of unrefined sugar or syrup. 780.819... STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15...

29 CFR 780.819 - Production must be of unrefined sugar or syrup.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false Production must be of unrefined sugar or syrup. 780.819... STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15...

Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion

PubMed Central

2011-01-01

Background Biomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment before enzymatic hydrolysis can begin. The optimal pretreatment can be different for different feedstocks, and should not lead to biomass destruction or formation of toxic products. Methods We examined the influence of six mild sulfuric acid or water pretreatments at different temperatures on the enzymatic degradability of sugar-beet pulp (SBP). Results We found that optimal pretreatment at 140°C of 15 minutes in water was able to solubilize 60% w/w of the total carbohydrates present, mainly pectins. More severe treatments led to the destruction of the solubilized sugars, and the subsequent production of the sugar-degradation products furfural, hydroxymethylfurfural, acetic acid and formic acid. The pretreated samples were successfully degraded enzymatically with an experimental cellulase preparation. Conclusions In this study, we found that pretreatment of SBP greatly facilitated the subsequent enzymatic degradation within economically feasible time ranges and enzyme levels. In addition, pretreatment of SBP can be useful to fractionate functional ingredients such as arabinans and pectins from cellulose. We found that the optimal combined severity factor to enhance the enzymatic degradation of SBP was between log R'0 = -2.0 and log R'0 = -1.5. The optimal pretreatment and enzyme treatment solubilized up to 80% of all sugars present in the SBP, including ≥90% of the cellulose. PMID:21627804

Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization

PubMed Central

Benoit, Isabelle; Zhou, Miaomiao; Vivas Duarte, Alexandra; Downes, Damien J.; Todd, Richard B.; Kloezen, Wendy; Post, Harm; Heck, Albert J. R.; Maarten Altelaar, A. F.; de Vries, Ronald P.

2015-01-01

Degradation of plant biomass to fermentable sugars is of critical importance for the use of plant materials for biofuels. Filamentous fungi are ubiquitous organisms and major plant biomass degraders. Single colonies of some fungal species can colonize massive areas as large as five soccer stadia. During growth, the mycelium encounters heterogeneous carbon sources. Here we assessed whether substrate heterogeneity is a major determinant of spatial gene expression in colonies of Aspergillus niger. We analyzed whole-genome gene expression in five concentric zones of 5-day-old colonies utilizing sugar beet pulp as a complex carbon source. Growth, protein production and secretion occurred throughout the colony. Genes involved in carbon catabolism were expressed uniformly from the centre to the periphery whereas genes encoding plant biomass degrading enzymes and nitrate utilization were expressed differentially across the colony. A combined adaptive response of carbon-catabolism and enzyme production to locally available monosaccharides was observed. Finally, our results demonstrate that A. niger employs different enzymatic tools to adapt its metabolism as it colonizes complex environments. PMID:26314379

Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization.

PubMed

Benoit, Isabelle; Zhou, Miaomiao; Vivas Duarte, Alexandra; Downes, Damien J; Todd, Richard B; Kloezen, Wendy; Post, Harm; Heck, Albert J R; Maarten Altelaar, A F; de Vries, Ronald P

2015-08-28

Degradation of plant biomass to fermentable sugars is of critical importance for the use of plant materials for biofuels. Filamentous fungi are ubiquitous organisms and major plant biomass degraders. Single colonies of some fungal species can colonize massive areas as large as five soccer stadia. During growth, the mycelium encounters heterogeneous carbon sources. Here we assessed whether substrate heterogeneity is a major determinant of spatial gene expression in colonies of Aspergillus niger. We analyzed whole-genome gene expression in five concentric zones of 5-day-old colonies utilizing sugar beet pulp as a complex carbon source. Growth, protein production and secretion occurred throughout the colony. Genes involved in carbon catabolism were expressed uniformly from the centre to the periphery whereas genes encoding plant biomass degrading enzymes and nitrate utilization were expressed differentially across the colony. A combined adaptive response of carbon-catabolism and enzyme production to locally available monosaccharides was observed. Finally, our results demonstrate that A. niger employs different enzymatic tools to adapt its metabolism as it colonizes complex environments.

Heterologous expression, purification and characterization of three novel esterases secreted by the lignocellulolytic fungus Penicillium purpurogenum when grown on sugar beet pulp.

PubMed

Oleas, Gabriela; Callegari, Eduardo; Sepúlveda, Romina; Eyzaguirre, Jaime

2017-04-18

The lignocellulolytic fungus, Penicillium purpurogenum, grows on a variety of natural carbon sources, among them sugar beet pulp. Culture supernatants of P. purpurogenum grown on sugar beet pulp were partially purified and the fractions obtained analyzed for esterase activity by zymograms. The bands with activity on methyl umbelliferyl acetate were subjected to mass spectrometry to identify peptides. The peptides obtained were probed against the proteins deduced from the genome sequence of P. purpurogenum. Eight putative esterases thus identified were chosen for future work. Their cDNAs were expressed in Pichia pastoris. The supernatants of the recombinant clones were assayed for esterase activity, and five of the proteins were active against one or more substrates: methyl umbelliferyl acetate, indoxyl acetate, methyl esterified pectin and fluorescein diacetate. Three of those enzymes were purified, further characterized and subjected to a BLAST search. Based on their amino acid sequence and properties, they were identified as follows: RAE1, pectin acetyl esterase (CAZy family CE 12); FAEA, feruloyl esterase (could not be assigned to a CAZy family) and EAN, acetyl esterase (former CAZy family CE 10). Copyright © 2017 Elsevier Ltd. All rights reserved.

Bleaching effects of massecuite on some properties of crystallized sugar beet and milk chocolate bars produced.

PubMed

Sadjadi, F Seyed; Honarvar, M; Kalbasi-Ashtari, A; Motaghian, P

2018-04-01

Four kinds of bleached, unbleached, second and third crystal sugars (BCS, UCS, SCS and TCS) were made from different massecuites in a sugar-beet factory, and their physiochemical (polarization, invert sugar, colorants, pH, ash and SO 2 ), microbiological and functional properties were measured. While the polarization of UCS, SCS and TCS were lower than BCS; their invert sucrose, colorants, pH and ash contents were significantly higher than BCS. The phenols and betaine of BCS, UCS, SCS, and TCS were 144, 401, 384 and 673 (mg/100 g); and 244, 791, 4662, and 6589 (mg/100 g); respectively. Whereas the phenol of milk chocolate bars (MCB) made with UCS, SCS, and TCS were only 10% higher than MCB completed with BCS; their betaine contents were substantially (up to 16 times) higher than the ones finished with BCS. Sensory evaluation showed that the MCB prepared with three sugars including UCS, SCS and TCS had significantly higher glossiness, brittleness, flavor and mouth feel than those made with BCS. The greater colorants, ash content and inverted sugars of UCS, SCS and TCS (in comparison with BCS) made considerable improvements in the glossiness, flavor and brittleness of MCB, respectively. BCS had 8 ppm of toxic sulfur; whereas, UCS, SCS and TCS had no detectable sulfur and significantly higher beneficial copper content than BCS. No pathogenic microorganism were detected in UCS, SCS, TCS or their subsequent MCB. Our results highly recommend using UCS, SCS and TCS instead of BCS in food products (such as MCB) due to their higher health benefits.

Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides.

PubMed

Ghazi, Iraj; Fernandez-Arrojo, Lucia; Gomez De Segura, Aranzazu; Alcalde, Miguel; Plou, Francisco J; Ballesteros, Antonio

2006-04-19

Sugar syrup and molasses from beet processing containing 620 and 570 mg/mL sucrose, respectively, were assayed as low-cost and available substrates for the enzymatic synthesis of fructo-oligosaccharides (FOSs). A commercial pectinase (Pectinex Ultra SP-L, from Aspergillus aculeatus) characterized by the presence of a transfructosylating activity was used as a biocatalyst. The FOS production increased when lowering the initial pH value of syrup (7.5) and molasses (8.9) to 5.5. Sugar syrup and molasses were diluted in order to reduce substrate viscosity; interestingly, the percentage of FOS with regards to total sugars remained almost constant, which indicated a high transferase-to-hydrolase ratio for this enzyme. Kinetics of FOS production was analyzed. Using approximately 10 U transfructosylating activity per g sucrose, the FOS concentration reached a maximum of 388 mg/mL after 30 h using syrup and 235 mg/mL in 65 h with molasses. These values corresponded to approximately 56 and 49% (w/w), respectively, of the total amount of carbohydrates in the mixture. The enzyme was also covalently immobilized on an epoxy-activated polymethacrylate-based polymer (Sepabeads EC-EP5). We found that immobilized Pectinex Ultra SP-L can be efficiently applied to the synthesis of FOS using syrup and molasses as substrates.

Interaction of soil pH and phosphorus efficacy: Long-term effects of P fertilizer and lime applications on wheat, barley, and sugar beet.

PubMed

von Tucher, Sabine; Hörndl, Dorothea; Schmidhalter, Urs

2018-01-01

Phosphorus (P), a plant macronutrient, must be adequately supplied for crop growth. In Germany, many soils are high in plant-available P; specifically in arable farming, P fertilizer application has been reduced or even omitted in the last decade. Therefore, it is important to understand how long these soils can support sustainable crop production, and what concentrations of soil P are required for it. We analyzed a 36-year long-term field experiment regarding the effects of different P application and liming rates on plant growth and soil P concentrations with a crop rotation of sugar beet, wheat, and barley. Sugar beet reacted to low soil P and low soil pH levels more sensitively than wheat, which was not significantly affected by the long-term omitted P application. All three crop species showed adequate growth at soil P levels lower than the currently recommended levels, if low soil pH was optimized by liming. The increase in efficacy of soil and fertilizer P by reduced P application rates therefore requires the adaptation of the soil pH to a soil type-specific optimal level.

Production of oxalic acid from sugar beet molasses by formed nitrogen oxides.

PubMed

Gürü, M; Bilgesü, A Y; Pamuk, V

2001-03-01

Production of oxalic acid from sugar beet molasses was developed in a series of three reactors. Nitrogen oxides formed were used to manufacture oxalic acid in the second and third reactor. Parameters affecting the reaction were determined to be, air flow rate, temperature, the amount of V2O5 catalyst and the concentrations of molasses and H2SO4. The maximum yields in the second and third reactors were 78.9% and 74.6% of theoretical yield, respectively. Also, kinetic experiments were performed and the first-order rate constants were determined for the glucose consumption rate. Nitrogen oxides in off-gases from the final reactor were absorbed in water and concentrated sulphuric acid and reused in the following reactors giving slightly lower yields under similar conditions. In this novel way, it was possible to recover NO(x) and to prevent air pollution. Meanwhile, it was possible to reduce the unit cost of reactant for oxalic acid production. A maximum 77.5% and 74.1% of theoretical yield was obtained by using the absorption solutions with NO(x).

Biosorption of Zn(II) from industrial effluents using sugar beet pulp and F. vesiculosus: From laboratory tests to a pilot approach.

PubMed

Castro, Laura; Blázquez, M Luisa; González, Felisa; Muñoz, Jesús A; Ballester, Antonio

2017-11-15

The aim of this work was to demonstrate the feasibility of the application of biosorption in the treatment of metal polluted wastewaters through the development of several pilot plants to be implemented by the industry. The use as biosorbents of both the brown seaweed Fucus vesiculosus and a sugar beet pulp was investigated to remove heavy metal ions from a wastewater generated in an electroplating industry: Industrial Goñabe (Valladolid, Spain). Batch experiments were performed to study the effects of pH, contact time and initial metal concentration on metal biosorption. It was observed that the adsorption capacity of the biosorbents strongly depended on the pH, increasing as the pH rises from 2 to 5. The adsorption kinetic was studied using three models: pseudo first order, pseudo second order and Elovich models. The experimental data were fitted to Langmuir and Freundlich isotherm models and the brown alga F. vesiculosus showed higher metal uptake than the sugar beet pulp. The biomasses were also used for zinc removal in fixed-bed columns. The performance of the system was evaluated in different experimental conditions. The mixture of the two biomasses, the use of serial columns and the inverse flow can be interesting attempts to improve the biosorption process for large-scale applications. Copyright © 2017 Elsevier B.V. All rights reserved.

Improved sugar beet pectin-stabilized emulsions through complexation with sodium caseinate.

PubMed

Li, Xiangyang; Fang, Yapeng; Phillips, Glyn O; Al-Assaf, Saphwan

2013-02-13

The study investigates the complexes formed between sodium caseinate (SC) and sugar beet pectin (SBP) and to harness them to stabilize SBP emulsions. We find that both hydrophobic and electrostatic interactions are involved in the complexation. In SC/SBP mixed solution, soluble SC/SBP complexes first form on acidification and then aggregate into insoluble complexes, which disassociate into soluble polymers upon further decreasing pH. The critical pH's for the formation of soluble and insoluble complexes and disappearance of insoluble complexes are designated as pH(c), pH(φ), and pH(d), respectively. These critical pH values define four regions in the phase diagram of complexation, and SC/SBP emulsions were prepared in these regions. The results show that the stability of SBP-stabilized emulsion is greatly improved at low SC/SBP ratios and acidic pH's. This enhancement can be attributed to an increase in the amount of adsorbed SBP as a result of cooperative adsorption to sodium caseinate. Using a low ratio of SC/SBP ensured that all caseinate molecules are completely covered by adsorbed SBP chains, which eliminates possible instability induced by thermal aggregation of caseinate molecules resulting from stress acceleration at elevated temperatures. A mechanistic model for the behavior is proposed.

The effect of glyphosate on import into a sink leaf of sugar beet

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shieh, Wenjang; Geiger, D.R.

1990-05-01

The basis for glyphosate inducted limitation of carbon import into developing leaves was studied in sugar beet. To separate the effects of the herbicide on export from those on import, glyphosate was supplied to a developing leaf from two exporting source leaves which fed the sink leaf. Carbon import into the sink leaf was determined by supplying {sup 14}CO{sub 2} to a third source leaf which also supplies carbon to the monitored sink leaf. Import into the sink leaf decreased within 2 to 3 h after glyphosate application, even though photosynthesis and export in the source leaf supplying {sup 14}Cmore » were unaffected. Reduced import into the sink leaf was accompanied by increased import by the tap root. Elongation of the sink leaf was only slightly decreased following arrival of glyphosate. Photosynthesis by the sink leaf was not inhibited. The results to data support the view that import is slowed by the inhibition of synthesis of structural or storage compounds in the developing leaves.« less

Influence of beet sugar, calcium lactate, and Staphylococcus xylosus (with nitrate reductase activity) on the chemical, microbiological, and sensorial properties of Persian uncured frankfurters.

PubMed

Tahmouzi, Saeed; Razavi, Seyed Hadi; Safari, Mohammad; Emam-Djomeh, Zahra

2012-10-01

This study investigated the effects of beet sugar (BS), calcium lactate (CL), and Staphylococcus xylosus (SX) on the general characteristics of uncured frankfurters. Minimum residual nitrite was observed in samples with a high level of BS and S. xylosus (8log(10) l0 viable cfu/g) after 2 mo. There was an increase in total aerobic counts in frankfurters after 4 wk. Samples containing higher BS and CL had no Clostridium perfringens at storage time. Histamine content was similar for all treatments except SX. No differences were observed in textural properties among the treatments. Water activity was decreased significantly in treatment CL1+BS2. The results indicated that samples treated with BS or CL had (P < 0.05) taste and sensory attributes considered appropriate by a tasting panel. Frankfurters are among the most popular meat products in many countries. Health problems (such as cancer) associated with the overconsumption of cured meat have led to searches for new, alternative methods of preservation. Hurdle technology (combinations of beet sugar, calcium lactate, and Staphylococcus xylosus in different concentrations) can be developed for frankfurter processing. Results of this research will be useful for meat manufacturers; however, any new process may affect the strategies chosen for marketing these products. © 2012 Institute of Food Technologists®

[The influence of colonizing methylobacteria on morphogenesis and resistance of sugar beet and white cabbage plants to Erwinia carotovora].

PubMed

Pigoleva, S V; Zakharchenko, N S; Pigolev, A V; Trotsenko, Iu A; Bur'ianov, Ia I

2009-01-01

The influence of colonization of sugar beet (Beta vulgaris var. saccharifera (Alef) Krass) and white cabbage (Brassica oleracea var. capitata L.) plants by methylotrophic bacteria Methylovorus mays on the growth, rooting, and plant resistance to phytopathogen bacteria Erwinia carotovora was investigated. The colonization by methylobacteria led to their steady association with the plants which had increased growth speed, root formation and photosynthetic activity. The colonized plants had increased resistance to Erwinia carotovora phytopathogen and were better adapted to greenhouse conditions. The obtained results showed the perspectives for the practical implementation of methylobacteria in the ecologically clean microbiology substances used as the plant growth stimulators and for the plant protection from pathogens.

21 CFR 73.40 - Dehydrated beets (beet powder).

Code of Federal Regulations, 2010 CFR

2010-04-01

... LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.40 Dehydrated beets (beet powder). (a) Identity. (1) The color additive dehydrated beets is a dark red powder prepared by dehydrating sound, mature, good quality, edible beets. (2) Color additive mixtures made with dehydrated beets may contain as...

Detection of adulteration in honey samples added various sugar syrups with 13C/12C isotope ratio analysis method.

PubMed

Tosun, Murat

2013-06-01

Honey can be adulterated in various ways. One of the adulteration methods is the addition of different sugar syrups during or after honey production. Starch-based sugar syrups, high fructose corn syrup (HFCS), glucose syrup (GS) and saccharose syrups (SS), which are produced from beet or canes, can be used for adulterating honey. In this study, adulterated honey samples were prepared with the addition of HFCS, GS and SS (beet sugar) at a ratio of 0%, 10%, 20%, 40% and 50% by weight. (13)C/(12)C analysis was conducted on these adulterated honey samples using an isotope ratio mass spectrometer in combination with an elemental analyser (EA-IRMS). As a result, adulteration using C(4) sugar syrups (HFCS and GS) could be detected to a certain extent while adulteration of honey using C(3) sugar syrups (beet sugar) could not be detected. Adulteration by using SS (beet sugar) still has a serious detection problem, especially in countries in which beet is used in manufacturing sugar. For this reason, practice and analysis methods are needed to meet this deficit and to detect the adulterations precisely in the studies that will be conducted. Copyright © 2012 Elsevier Ltd. All rights reserved.

Immobilized Sclerotinia sclerotiorum invertase to produce invert sugar syrup from industrial beet molasses by-product.

PubMed

Mouelhi, Refka; Abidi, Ferid; Galai, Said; Marzouki, M Nejib

2014-03-01

The fungus Sclerotinia sclerotiorum produces invertase activity during cultivation on many agroindustrial residues. The molasses induced invertase was purified by DEAE-cellulose chromatography. The molecular mass of the purified enzyme was estimated at 48 kDa. Optimal temperature was determined at 60 °C and thermal stability up to 65 °C. The enzyme was stable between pH 2.0 and 8.0; optimum pH was about 5.5. Apparent K(m) and V(max) for sucrose were estimated to be respectively 5.8 mM and 0.11 μmol/min. The invertase was activated by β-mercaptoethanol. Free enzyme exhibited 80 % of its original activity after two month's storage at 4 °C and 50 % after 1 week at 25 °C. In order to investigate an industrial application, the enzyme was immobilized on alginate and examined for invert sugar production by molasses hydrolysis in a continuous bioreactor. The yield of immobilized invertase was about 78 % and the activity yield was 59 %. Interestingly the immobilized enzyme hydrolyzed beet molasses consuming nearly all sucrose. It retained all of its initial activity after being used for 4 cycles and about 65 % at the sixth cycle. Regarding productivity; 20 g/l of molasses by-product gave the best invert sugar production 46.21 g/day/100 g substrate related to optimal sucrose conversion of 41.6 %.

Impact of extra virgin olive oil and ethylenediaminetetraacetic acid (EDTA) on the oxidative stability of fish oil emulsions and spray-dried microcapsules stabilized by sugar beet pectin.

PubMed

Polavarapu, Sudheera; Oliver, Christine M; Ajlouni, Said; Augustin, Mary Ann

2012-01-11

The influence of EDTA on lipid oxidation in sugar beet pectin-stabilized oil-in-water emulsions (pH 6, 15% oil, wet basis), prepared from fish oil (FO) and fish oil-extra virgin olive oil (FO-EVOO) (1:1 w/w), as well as the spray-dried microcapsules (50% oil, dry basis) prepared from these emulsions, was investigated. Under accelerated conditions (80 °C, 5 bar oxygen pressure) the oxidative stability was significantly (P < 0.05) higher for FO and FO-EVOO formulated with EDTA, in comparison to corresponding emulsions and spray-dried microcapsules formulated without EDTA. The EDTA effect was greater in emulsions than in spray-dried microcapsules, with the greatest protective effect obtained in FO-EVOO emulsions. EDTA enhanced the oxidative stability of the spray-dried microcapsules during ambient storage (~25 °C, a(w) = 0.5), as demonstrated by their lower concentration of headspace volatile oxidation products, propanal and hexanal. These results show that the addition of EDTA is an effective strategy to maximize the oxidative stability of both FO emulsions and spray-dried microcapsules in which sugar beet pectin is used as the encapsulant material.

Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production.

PubMed

Berlowska, Joanna; Cieciura, Weronika; Borowski, Sebastian; Dudkiewicz, Marta; Binczarski, Michal; Witonska, Izabela; Otlewska, Anna; Kregiel, Dorota

2016-10-17

Research into fermentative production of lactic acid from agricultural by-products has recently concentrated on the direct conversion of biomass, whereby pure sugars are replaced with inexpensive feedstock in the process of lactic acid production. In our studies, for the first time, the source of carbon used is sugar beet pulp, generated as a by-product of industrial sugar production. In this paper, we focus on the simultaneous saccharification of lignocellulosic biomass and fermentation of lactic acid, using mixed cultures with complementary assimilation profiles. Lactic acid is one of the primary platform chemicals, and can be used to synthesize a wide variety of useful products, including green propylene glycol. A series of controlled batch fermentations was conducted under various conditions, including pretreatment with enzymatic hydrolysis. Inoculation was performed in two sequential stages, to avoid carbon catabolite repression. Biologically-synthesized lactic acid was catalytically reduced to propylene glycol over 5% Ru/C. The highest lactic acid yield was obtained with mixed cultures. The yield of propylene glycol from the biological lactic acid was similar to that obtained with a water solution of pure lactic acid. Our results show that simultaneous saccharification and fermentation enables generation of lactic acid, suitable for further chemical transformations, from agricultural residues.

Suppressed expression of choline monooxygenase in sugar beet on the accumulation of glycine betaine.

PubMed

Yamada, Nana; Takahashi, Hiroyuki; Kitou, Kunihide; Sahashi, Kosuke; Tamagake, Hideto; Tanaka, Yoshito; Takabe, Teruhiro

2015-11-01

Glycine betaine (GB) is an important osmoprotectant and synthesized by two-step oxidation of choline. Choline monooxygenase (CMO) catalyzes the first step of the pathway and is believed to be a rate limiting step for GB synthesis. Recent studies have shown the importance of choline-precursor supply for GB synthesis. In order to investigate the role of CMO for GB accumulation in sugar beet (Beta vulgaris), transgenic plants carrying the antisense BvCMO gene were developed. The antisense BvCMO plants showed the decreased activity of GB synthesis from choline compared to wild-type (WT) plants which is well related to the suppressed level of BvCMO protein. However, GB contents were similar between transgenic and WT plants with the exception of young leaves and storage roots. Transgenic plants showed enhanced susceptibility to salt stress than WT plants. These results suggest the importance of choline-precursor-supply for GB accumulation, and young leaves and storage root are sensitive sites for GB accumulation. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

Stable operation during pilot-scale anaerobic digestion of nutrient-supplemented maize/sugar beet silage.

PubMed

Nges, Ivo Achu; Björn, Annika; Björnsson, Lovisa

2012-08-01

Biogas production from maize/sugar beet silage was studied under mesophilic conditions in a continuous stirred tank reactor pilot-scale process. While energy crop mono-digestion is often performed with very long hydraulic retention times (HRTs), the present study demonstrated an efficient process operating with a 50-day HRT and a corrected total solids (TS(corr)) based organic loading rate of 3.4 kg/m(3)d. The good performance was attributed to supplementation with both macro- and micronutrients and was evidenced by good methane yields (318 m(3)/ton TS(corr)), which were comparable to laboratory maximum expected yields, plus low total volatile fatty acid concentrations (<0.8 g/L). A viscoplastic and thixotropic digester fluid behaviour was observed, and the viscosity problems common in crop mono-digestion were not seen in this study. The effluent also complied with Swedish certification standards for bio-fertilizer for farmland application. Nutrient addition thus rendered a stable biogas process, while the effluent was a good quality bio-fertilizer. Copyright © 2012 Elsevier Ltd. All rights reserved.

Combinatorial control of gene expression in Aspergillus niger grown on sugar beet pectin.

PubMed

Kowalczyk, Joanna E; Lubbers, Ronnie J M; Peng, Mao; Battaglia, Evy; Visser, Jaap; de Vries, Ronald P

2017-09-27

Aspergillus niger produces an arsenal of extracellular enzymes that allow synergistic degradation of plant biomass found in its environment. Pectin is a heteropolymer abundantly present in the primary cell wall of plants. The complex structure of pectin requires multiple enzymes to act together. Production of pectinolytic enzymes in A. niger is highly regulated, which allows flexible and efficient capture of nutrients. So far, three transcriptional activators have been linked to regulation of pectin degradation in A. niger. The L-rhamnose-responsive regulator RhaR controls the production of enzymes that degrade rhamnogalacturonan-I. The L-arabinose-responsive regulator AraR controls the production of enzymes that decompose the arabinan and arabinogalactan side chains of rhamnogalacturonan-II. The D-galacturonic acid-responsive regulator GaaR controls the production of enzymes that act on the polygalacturonic acid backbone of pectin. This project aims to better understand how RhaR, AraR and GaaR co-regulate pectin degradation. For that reason, we constructed single, double and triple disruptant strains of these regulators and analyzed their growth phenotype and pectinolytic gene expression in A. niger grown on sugar beet pectin.

The Acquisition of Indigenous Plasmids by a Genetically Marked Pseudomonad Population Colonizing the Sugar Beet Phytosphere Is Related to Local Environmental Conditions

PubMed Central

Lilley, A. K.; Bailey, M. J.

1997-01-01

The transfer of naturally occurring conjugative plasmids from the indigenous microflora to a genetically modified population of bacteria colonizing the phytospheres of plants has been observed. The marked strain (Pseudomonas fluorescens SBW25EeZY6KX) was introduced as a seed dressing to sugar beets (Beta vulgaris var. Amethyst) as part of a field experiment to assess the ecology and genetic stability of deliberately released bacterial inocula. The sustained populations of the introduced strain, which colonized the phytosphere, were assessed throughout the growing season for the acquisition of plasmids conferring mercury resistance (Hg(supr)). Transconjugants were isolated only from root and leaf samples collected within a narrow temporal window coincident with the midseason maturation of the crop. Conjugal-transfer events were recorded during this defined period in two separate field release experiments conducted over consecutive years. On one occasion seven of nine individual plants sampled supported transconjugant P. fluorescens SBW25EeZY6KX, demonstrating that conjugative gene transfer between bacterial populations in the phytosphere may be a common event under specific environmental conditions. The plasmids acquired in situ by the colonizing inocula were identified as natural variants of restriction digest pattern group I, III, or IV plasmids from five genetically distinct groups of large, conjugative mercury resistance plasmids known to persist in the phytospheres of sugar beets at the field site. These data demonstrate not only that gene transfer may be a common event but also that the genetic and phenotypic stability of inocula released into the natural environment cannot be predicted. PMID:16535580

Vermistabilization of sugar beet (Beta vulgaris L) waste produced from sugar factory using earthworm Eisenia fetida: Genotoxic assessment by Allium cepa test.

PubMed

Bhat, Sartaj Ahmad; Singh, Jaswinder; Vig, Adarsh Pal

2015-08-01

In the present study, sugar beet mud (SBM) and pulp (SBP) produced as a waste by-products of the sugar industry were mixed with cattle dung (CD) at different ratios on dry weight basis for vermicomposting with Eisenia fetida. Minimum mortality and highest population of worms were observed in 20:80 (SBM20) mixture of SBM and 10:90 (SBP10) ratios. However, increased percentages of wastes significantly affected the growth and fecundity of worms. Nutrients like nitrogen, phosphorus, sodium, increased from initial feed mixture to final products (i.e., vermicompost), while organic carbon (OC), C:N ratio and electrical conductivity (EC) declined in all the products of vermicomposting. Although there was an increase in the contents of all the heavy metals except copper, chromium, and iron in SBM, the contents were less than the international standards for compost which indicates that the vermicompost can be used in the fields without any ill effects on the soil. Allium cepa root chromosomal aberration assay was used to evaluate the genotoxicity of pre- and post-vermicomposted SBM to understand the effect of vermicomposting on the reduction of toxicity. Genotoxicity analysis of post-vermicomposted samples of SBM revealed 18-75% decline in the aberration frequencies. Scanning electron microscopy (SEM) was recorded to identify the changes in texture in the control and vermicomposted samples. The vermicomposted mixtures in the presence of earthworms confirm more numerous surface irregularities that prove to be good manure.

Effect of sugar beet genotype on the Beet necrotic yellow vein virus P25 pathogenicity factor and evidence for a fitness penalty in resistance-breaking strains.

PubMed

Bornemann, Kathrin; Varrelmann, Mark

2013-05-01

Beet necrotic yellow vein virus (BNYVV), vectored by Polymyxa betae, causes rhizomania in sugar beet. For disease control, the cultivation of hybrids carrying Rz1 resistance is crucial, but is compromised by resistance-breaking (RB) strains with specific mutations in the P25 protein at amino acids 67-70 (tetrad). To obtain evidence for P25 variability from soil-borne populations, where the virus persists for decades, populations with wild-type (WT) and RB properties were analysed by P25 deep sequencing. The level of P25 variation in the populations analysed did not correlate with RB properties. Remarkably, one WT population contained P25 with RB mutations at a frequency of 11%. To demonstrate selection by Rz1 and the influence of RB mutations on relative fitness, competition experiments between strains were performed. Following a mixture of strains with four RNAs, a shift in tetrad variants was observed, suggesting that strains did not mix or transreplicate. The plant genotype exerted a clear influence on the frequency of RB tetrads. In Rz1 plants, the RB variants outcompeted the WT variants, and mostly vice versa in susceptible plants, demonstrating a relative fitness penalty of RB mutations. The strong genotype effect supports the hypothesized Rz1 RB strain selection with four RNAs, suggesting that a certain tetrad needs to become dominant in a population to influence its properties. Tetrad selection was not observed when an RB strain, with an additional P26 protein encoded by a fifth RNA, competed with a WT strain, supporting its role as a second BNYVV pathogenicity factor and suggesting the reassortment of both types. © 2013 BSPP AND BLACKWELL PUBLISHING LTD.

Leaching of the Neonicotinoids Thiamethoxam and Imidacloprid from Sugar Beet Seed Dressings to Subsurface Tile Drains.

PubMed

Wettstein, Felix E; Kasteel, Roy; Garcia Delgado, Maria F; Hanke, Irene; Huntscha, Sebastian; Balmer, Marianne E; Poiger, Thomas; Bucheli, Thomas D

2016-08-24

Pesticide transport from seed dressings toward subsurface tile drains is still poorly understood. We monitored the neonicotinoid insecticides imidacloprid and thiamethoxam from sugar beet seed dressings in flow-proportional drainage water samples, together with spray applications of bromide and the herbicide S-metolachlor in spring and the fungicides epoxiconazole and kresoxim-methyl in summer. Event-driven, high first concentration maxima up to 2830 and 1290 ng/L for thiamethoxam and imidacloprid, respectively, were followed by an extended period of tailing and suggested preferential flow. Nevertheless, mass recoveries declined in agreement with the degradation and sorption properties collated in the groundwater ubiquity score, following the order bromide (4.9%), thiamethoxam (1.2%), imidacloprid (0.48%), kresoxim-methyl acid (0.17%), S-metolachlor (0.032%), epoxiconazole (0.013%), and kresoxim-methyl (0.003%), and indicated increased leaching from seed dressings compared to spray applications. Measured concentrations and mass recoveries indicate that subsurface tile drains contribute to surface water contamination with neonicotinoids from seed dressings.

Comparison of mesophilic and thermophilic anaerobic digestion of sugar beet pulp: performance, dewaterability and foam control.

PubMed

Suhartini, Sri; Heaven, Sonia; Banks, Charles J

2014-01-01

Digestion of sugar beet pulp was assessed in relation to biogas and methane production, foaming potential, and digestate dewaterability. Four 4-litre working volume digesters were operated mesophilically (37±0.5 °C) and four thermophilically (55±0.5 °C) over three hydraulic retention times. Digesters were operated in duplicate at organic loading rates (OLR) of 4 and 5 g volatile solids l(-1) day(-1) without water addition. Thermophilic digestion gave higher biogas and methane productivity than mesophilic and was able to operate at the higher OLR, where mesophilic digestion showed signs of instability. Digestate dewaterability was assessed using capillary suction time and frozen image centrifugation. The occurrence of, or potential for, stable foam formation was assessed using a foaming potential test. Thermophilic operation allowed higher loadings to be applied without loss of performance, and gave a digestate with superior dewatering characteristics and very little foaming potential. Copyright © 2013 Elsevier Ltd. All rights reserved.

Sugar (sucrose) holograms

NASA Astrophysics Data System (ADS)

Ponce-Lee, E. L.; Olivares-Pérez, A.; Fuentes-Tapia, I.

2004-06-01

Computer holograms made with sugar crystals are reported. This material is well known as a good sweetener; the sugar from sugar cane or sugar beet (sucrose). These sweetener can be applied as honey "water and diluted sugar" easily on any substrate such as plastics or glasses without critical conditions for developed process. This step corresponds only to the cured sucrose as a photopolymer process. The maximum absorption spectra is localized at UV region λ=240 nm. We record with lithographic techniques some gratings, showing a good diffraction efficiency around 45%. This material has good resolution to make diffraction gratings. These properties are attractive because they open the possibility to make phase holograms on candies. Mainly the phase modulation is by refraction index.

Beet Juice-Induced Green Fabrication of Plasmonic AgCl/Ag Nanoparticles

EPA Science Inventory

A simple, green, and fast approach (complete within 5 min) was explored for the fabrication of hybrid AgCl/Ag plasmonic nanoparticles under microwave (MW) irradiation. In this method, beet juice served as a reducing reagent, which is an abundant sugar-rich agricultural produce. I...

Feeding of wheat bran and sugar beet pulp as sole supplements in high-forage diets emphasizes the potential of dairy cattle for human food supply.

PubMed

Ertl, P; Zebeli, Q; Zollitsch, W; Knaus, W

2016-02-01

Besides the widely discussed negative environmental effects of dairy production, such as greenhouse gas emissions, the feeding of large amounts of potentially human-edible feedstuffs to dairy cows is another important sustainability concern. The aim of this study was therefore to investigate the effects of a complete substitution of common cereal grains and pulses with a mixture of wheat bran and sugar beet pulp in a high-forage diet on cow performance, production efficiency, feed intake, and ruminating behavior, as well as on net food production potential. Thirteen multiparous and 7 primiparous mid-lactation Holstein dairy cows were randomly assigned to 1 of 2 treatments in a change-over design with 7-wk periods. Cows were fed a high-forage diet (grass silage and hay accounted for 75% of the dry matter intake), supplemented with either a cereal grain-based concentrate mixture (CON), or a mixture of wheat bran and dried sugar beet pulp (WBBP). Human-edible inputs were calculated for 2 different scenarios based on minimum and maximum potential recovery rates of human-edible energy and protein from the respective feedstuffs. Dietary starch and neutral detergent fiber contents were 3.0 and 44.1% for WBBP, compared with 10.8 and 38.2% in CON, respectively. Dietary treatment did not affect milk production, milk composition, feed intake, or total chewing activity. However, chewing index expressed in minutes per kilogram of neutral detergent fiber ingested was 12% lower in WBBP compared with CON. In comparison to CON, the human-edible feed conversion efficiencies for energy and protein, defined as human-edible output per human-edible input, were 6.8 and 5.3 times higher, respectively, in WBBP under the maximum scenario. For the maximum scenario, the daily net food production (human-edible output minus human-edible input) increased from 5.4 MJ and 250 g of crude protein per cow in CON to 61.5 MJ and 630 g of crude protein in the WBBP diet. In conclusion, our data suggest

Effect of quality and origin of technical sucrose solutions on the inclusion of colourants into the sugar crystal matrix.

PubMed

Schlumbach, Karl; Scharfe, Maria; Flöter, Eckhard

2018-06-01

Due to the liberalisation of the European sugar market the pressure to improve factory utilisation is growing. Currently, beet and cane as sucrose sources are produced in isolation, according to geography. Co-production of sugar from beet and cane origin in one stream is a promising option. However, the knowledge base for production sugar is practically non-existent. This paper is part of our contribution to this field and specifically addresses effects of raw material quality. This framework formulated for the colouring of sugars crystallised from mixed syrups is also valid for different raw material qualities: raw cane sugars: colour values 1221 to 2505 IU, dextran levels 50 to 1200 mg kg -1 ; beet syrups: 1509 to 2058 IU. Co-crystallisation is the main colour incorporation mechanism. Colour due to liquid inclusion increases strongly at cane inclusion levels in excess of 60%. The prediction of final sugar colour based on characteristics of pure mixture constituents is verified and indicates significant differences to current recommendations. A first comprehensive description of the colour values in sugars produced from mixed cane and beet syrups is presented. Prediction of colour values from data on sugar colour of single sources marks a major contribution to future applications of co-production. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Populations of weedy crop–wild hybrid beets show contrasting variation in mating system and population genetic structure

PubMed Central

Arnaud, Jean-François; Fénart, Stéphane; Cordellier, Mathilde; Cuguen, Joël

2010-01-01

Reproductive traits are key parameters for the evolution of invasiveness in weedy crop–wild hybrids. In Beta vulgaris, cultivated beets hybridize with their wild relatives in the seed production areas, giving rise to crop–wild hybrid weed beets. We investigated the genetic structure, the variation in first-year flowering and the variation in mating system among weed beet populations occurring within sugar beet production fields. No spatial genetic structure was found for first-year populations composed of F1 crop–wild hybrid beets. In contrast, populations composed of backcrossed weed beets emerging from the seed bank showed a strong isolation-by-distance pattern. Whereas gametophytic self-incompatibility prevents selfing in wild beet populations, all studied weed beet populations had a mixed-mating system, plausibly because of the introgression of the crop-derived Sf gene that disrupts self-incompatibility. No significant relationship between outcrossing rate and local weed beet density was found, suggesting no trends for a shift in the mating system because of environmental effects. We further reveal that increased invasiveness of weed beets may stem from positive selection on first-year flowering induction depending on the B gene inherited from the wild. Finally, we discuss the practical and applied consequences of our findings for crop-weed management. PMID:25567926

Sequence-based analysis of pQBR103; a representative of a unique, transfer-proficient mega plasmid resident in the microbial community of sugar beet

PubMed Central

Tett, Adrian; Spiers, Andrew J; Crossman, Lisa C; Ager, Duane; Ciric, Lena; Dow, J Maxwell; Fry, John C; Harris, David; Lilley, Andrew; Oliver, Anna; Parkhill, Julian; Quail, Michael A; Rainey, Paul B; Saunders, Nigel J; Seeger, Kathy; Snyder, Lori AS; Squares, Rob; Thomas, Christopher M; Turner, Sarah L; Zhang, Xue-Xian; Field, Dawn; Bailey, Mark J

2009-01-01

The plasmid pQBR103 was found within Pseudomonas populations colonizing the leaf and root surfaces of sugar beet plants growing at Wytham, Oxfordshire, UK. At 425 kb it is the largest self-transmissible plasmid yet sequenced from the phytosphere. It is known to enhance the competitive fitness of its host, and parts of the plasmid are known to be actively transcribed in the plant environment. Analysis of the complete sequence of this plasmid predicts a coding sequence (CDS)-rich genome containing 478 CDSs and an exceptional degree of genetic novelty; 80% of predicted coding sequences cannot be ascribed a function and 60% are orphans. Of those to which function could be assigned, 40% bore greatest similarity to sequences from Pseudomonas spp, and the majority of the remainder showed similarity to other c-proteobacterial genera and plasmids. pQBR103 has identifiable regions presumed responsible for replication and partitioning, but despite being tra+ lacks the full complement of any previously described conjugal transfer functions. The DNA sequence provided few insights into the functional significance of plant-induced transcriptional regions, but suggests that 14% of CDSs may be expressed (11 CDSs with functional annotation and 54 without), further highlighting the ecological importance of these novel CDSs. Comparative analysis indicates that pQBR103 shares significant regions of sequence with other plasmids isolated from sugar beet plants grown at the same geographic location. These plasmid sequences indicate there is more novelty in the mobile DNA pool accessible to phytosphere pseudomonas than is currently appreciated or understood. PMID:18043644

Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants.

PubMed

Chiba, Soutaro; Miyanishi, Masaki; Andika, Ida Bagus; Kondo, Hideki; Tamada, Tetsuo

2008-05-01

The RNA3-encoded p25 protein of beet necrotic yellow vein virus (BNYVV) is responsible for the production of rhizomania symptoms of sugar beet roots (Beta vulgaris subsp. vulgaris). Here, it was found that the presence of the p25 protein is also associated with the resistance response in rub-inoculated leaves of sugar beet and wild beet (Beta vulgaris subsp. maritima) plants. The resistance phenotype displayed a range of symptoms from no visible lesions to necrotic or greyish lesions at the inoculation site, and only very low levels of virus and viral RNA accumulated. The susceptible phenotype showed large, bright yellow lesions and developed high levels of virus accumulation. In roots after Polymyxa betae vector inoculation, however, no drastic differences in virus and viral RNA accumulation levels were found between plants with susceptible and resistant phenotypes, except at an early stage of infection. There was a genotype-specific interaction between BNYVV strains and two selected wild beet lines (MR1 and MR2) and sugar beet cultivars. Sequence analysis of natural BNYVV isolates and site-directed mutagenesis of the p25 protein revealed that 3 aa residues at positions 68, 70 and 179 are important in determining the resistance phenotype, and that host-genotype specificity is controlled by single amino acid changes at position 68. The mechanism of the occurrence of resistance-breaking BNYVV strains is discussed.

Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by "Vitreoscilla hemoglobin expressing" Escherichia coli.

PubMed

Akbas, Meltem Yesilcimen; Sar, Taner; Ozcelik, Busra

2014-01-01

This work investigated the improvement of ethanol production by engineered ethanologenic Escherichia coli to express the hemoglobin from the bacterium Vitreoscilla (VHb). Ethanologenic E. coli strain FBR5 and FBR5 transformed with the VHb gene in two constructs (strains TS3 and TS4) were grown in cheese whey (CW) medium at small and large scales, at both high and low aeration, or with whey powder (WP) or sugar beet molasses hydrolysate (SBMH) media at large scale and low aeration. Culture pH, cell growth, VHb levels, and ethanol production were evaluated after 48 h. VHb expression in TS3 and TS4 enhanced their ethanol production in CW (21-419%), in WP (17-362%), or in SBMH (48-118%) media. This work extends the findings that "VHb technology" may be useful for improving the production of ethanol from waste and byproducts of various sources.

Combination of sugar and stable isotopes analyses to detect the use of non-grape sugars in balsamic vinegar must.

PubMed

Matteo, Perini; Tiziana, Nardin; Federica, Camin; Mario, Malacarne; Roberto, Larcher

2018-06-15

'Aceto Balsamico di Modena' (ABM) is a PGI (Protected Geographical Indication) salad dressing obtained from cooked and/or concentrated grape must, with the addition of wine vinegar and a maximum of 2% caramel (EU Reg. 583/2009). In this study we investigated whether the combination of 13 C/ 12 C of ethanol using Isotope Ratio Mass Spectrometry with 2 H-site-specific Natural Isotope Fractionation - Nuclear Magnetic Resonance, and minor sugars using Ion Chromatography with Pulse Amperometric and Charged Aerosol Detection, is able to improve detection of sugar addition to ABM must. A large selection of authentic Italian grape musts and different samples of balsamic vinegar with an increasing percentage of added beet, cane and sugar syrups were considered. The possible degradation of sugars in the ABM matrix during shelf life was also investigated. While stable isotope ratios analysis remains the favoured method for determining cane and beet sugar addition, dosage of minor sugar (in particular maltose) proved to be very useful for detecting the addition of sugar syrup. Thanks to this innovative approach, 3 out of 27 commercial ABMs were identified as adulterated. A combination of stable isotope ratio and IC-PAD-CAD analysis can be therefore proposed as a suitable tool for detecting the authenticity of ABM must. This article is protected by copyright. All rights reserved.

Diversification, evolution and methylation of short interspersed nuclear element families in sugar beet and related Amaranthaceae species.

PubMed

Schwichtenberg, Katrin; Wenke, Torsten; Zakrzewski, Falk; Seibt, Kathrin M; Minoche, André; Dohm, Juliane C; Weisshaar, Bernd; Himmelbauer, Heinz; Schmidt, Thomas

2016-01-01

Short interspersed nuclear elements (SINEs) are non-autonomous non-long terminal repeat retrotransposons which are widely distributed in eukaryotic organisms. While SINEs have been intensively studied in animals, only limited information is available about plant SINEs. We analysed 22 SINE families from seven genomes of the Amaranthaceae family and identified 34 806 SINEs, including 19 549 full-length copies. With the focus on sugar beet (Beta vulgaris), we performed a comparative analysis of the diversity, genomic and chromosomal organization and the methylation of SINEs to provide a detailed insight into the evolution and age of Amaranthaceae SINEs. The lengths of consensus sequences of SINEs range from 113 nucleotides (nt) up to 224 nt. The SINEs show dispersed distribution on all chromosomes but were found with higher incidence in subterminal euchromatic chromosome regions. The methylation of SINEs is increased compared with their flanking regions, and the strongest effect is visible for cytosines in the CHH context, indicating an involvement of asymmetric methylation in the silencing of SINEs. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.

cDNA cloning and analysis of betaine aldehyde dehydrogenase, a salt inducible enzyme in sugar beet

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCue, K.F.; Hanson, A.D.

1990-05-01

Betaine accumulates and serves as a compatible osmolyte in some plants subjected to drought or salinity stress. The last enzyme in the betaine biosynthetic pathway is betaine aldehyde dehydrogenase (BADH). The activity of BADH increases in response to increasing salinity levels. This increase in activity corresponds to an increase in protein detectable by immunoblotting, and to an increase in the translatable BADH mRNA. BADH was cloned from a cDNA library constructed in {lambda}gt10 using poly(A){sup +} RNA from sugar beets salinized to 500 mM NaCl. cDNAs were size selected (>1kb) before ligation into the vector, and the library was screenedmore » with a spinach BADH cDNA probe. Three nearly full length clones obtained were confirmed as BADH by their nucleotide and deduced amino acid homology to spinach BADH. Clones averaged 1.8 kb and contained open reading frames of 500 amino acids at 80% identity with spinach BADH. RNA gel blot analysis of poly(A){sup +} RNA indicated that salinization to 500 mM NaCl resulted in a 5-fold increase of BADH mRNA level.« less

Cloning of a cystatin gene from sugar beet M14 that can enhance plant salt tolerance.

PubMed

Wang, Yuguang; Zhan, Yanan; Wu, Chuan; Gong, Shilong; Zhu, Ning; Chen, Sixue; Li, Haiying

2012-08-01

An open reading frame encoding a cysteine protease inhibitor, cystatin was isolated from the buds of sugar beet monosomic addition line M14 (BvM14) using 5'-/3'-RACE method. It encoded a polypeptide of 104 amino acids with conserved G and PW motifs, the consensus phytocystatin sequence LARFAV and the active site QVVAG. The protein showed significant homology to other plant cystatins. BvM14-cystatin was expressed ubiquitously in roots, stems, leaves and flower tissues with relatively high abundance in developing stems and roots. It was found to be localized in the nucleus, cytoplasm and plasma membrane. Recombinant BvM14-cystatin expressed in Escherichia coli was purified and it exhibited cysteine protease inhibitor activity. Salt-stress treatment induced BvM14-cystatin transcript levels in the M14 seedlings. Homozygous Arabidopsis plants over-expressing BvM14-cystatin showed enhanced salt tolerance. Taken together, these data improved understanding of the functions of BvM14-cystatin and highlighted the possibility of employing the cystatin in engineering plants for enhanced salt tolerance. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

The effects of foliar fertilization with iron sulfate in chlorotic leaves are limited to the treated area. A study with peach trees (Prunus persica L. Batsch) grown in the field and sugar beet (Beta vulgaris L.) grown in hydroponics

PubMed Central

El-Jendoubi, Hamdi; Vázquez, Saúl; Calatayud, Ángeles; Vavpetič, Primož; Vogel-Mikuš, Katarina; Pelicon, Primož; Abadía, Javier; Abadía, Anunciación; Morales, Fermín

2014-01-01

Crop Fe deficiency is a worldwide problem. The aim of this study was to assess the effects of foliar Fe applications in two species grown in different environments: peach (Prunus persica L. Batsch) trees grown in the field and sugar beet (Beta vulgaris L. cv. “Orbis”) grown in hydroponics. The distal half of Fe-deficient, chlorotic leaves was treated with Fe sulfate by dipping and using a brush in peach trees and sugar beet plants, respectively. The re-greening of the distal (Fe-treated) and basal (untreated) leaf areas was monitored, and the nutrient and photosynthetic pigment composition of the two areas were also determined. Leaves were also studied using chlorophyll fluorescence imaging, low temperature-scanning electron microscopy microanalysis, scanning transmission ion microscopy-particle induced X-ray emission and Perls Fe staining. The distal, Fe-treated leaf parts of both species showed a significant increase in Fe concentrations (across the whole leaf volume) and marked re-greening, with significant increases in the concentrations of all photosynthetic pigments, as well as decreases in de-epoxidation of xanthophyll cycle carotenoids and increases in photochemical efficiency. In the basal, untreated leaf parts, Fe concentrations increased slightly, but little re-greening occurred. No changes in the concentrations of other nutrients were found. Foliar Fe fertilization was effective in re-greening treated leaf areas both in peach trees and sugar beet plants. Results indicate that the effects of foliar Fe-sulfate fertilization in Fe-deficient, chlorotic leaves were minor outside the leaf surface treated, indicating that Fe mobility within the leaf is a major constraint for full fertilizer effectiveness in crops where Fe-deficiency is established and leaf chlorosis occurs. PMID:24478782

The effects of foliar fertilization with iron sulfate in chlorotic leaves are limited to the treated area. A study with peach trees (Prunus persica L. Batsch) grown in the field and sugar beet (Beta vulgaris L.) grown in hydroponics.

PubMed

El-Jendoubi, Hamdi; Vázquez, Saúl; Calatayud, Angeles; Vavpetič, Primož; Vogel-Mikuš, Katarina; Pelicon, Primož; Abadía, Javier; Abadía, Anunciación; Morales, Fermín

2014-01-01

Crop Fe deficiency is a worldwide problem. The aim of this study was to assess the effects of foliar Fe applications in two species grown in different environments: peach (Prunus persica L. Batsch) trees grown in the field and sugar beet (Beta vulgaris L. cv. "Orbis") grown in hydroponics. The distal half of Fe-deficient, chlorotic leaves was treated with Fe sulfate by dipping and using a brush in peach trees and sugar beet plants, respectively. The re-greening of the distal (Fe-treated) and basal (untreated) leaf areas was monitored, and the nutrient and photosynthetic pigment composition of the two areas were also determined. Leaves were also studied using chlorophyll fluorescence imaging, low temperature-scanning electron microscopy microanalysis, scanning transmission ion microscopy-particle induced X-ray emission and Perls Fe staining. The distal, Fe-treated leaf parts of both species showed a significant increase in Fe concentrations (across the whole leaf volume) and marked re-greening, with significant increases in the concentrations of all photosynthetic pigments, as well as decreases in de-epoxidation of xanthophyll cycle carotenoids and increases in photochemical efficiency. In the basal, untreated leaf parts, Fe concentrations increased slightly, but little re-greening occurred. No changes in the concentrations of other nutrients were found. Foliar Fe fertilization was effective in re-greening treated leaf areas both in peach trees and sugar beet plants. Results indicate that the effects of foliar Fe-sulfate fertilization in Fe-deficient, chlorotic leaves were minor outside the leaf surface treated, indicating that Fe mobility within the leaf is a major constraint for full fertilizer effectiveness in crops where Fe-deficiency is established and leaf chlorosis occurs.

Combination of sugar analysis and stable isotope ratio mass spectrometry to detect the use of artificial sugars in royal jelly production.

PubMed

Wytrychowski, Marine; Daniele, Gaëlle; Casabianca, Hervé

2012-05-01

The effects of feeding bees artificial sugars and/or proteins on the sugar compositions and (13)C isotopic measurements of royal jellies (RJs) were evaluated. The sugars fed to the bees were two C4 sugars (cane sugar and maize hydrolysate), two C3 sugars (sugar beet, cereal starch hydrolysate), and honey. The proteins fed to them were pollen, soybean, and yeast powder proteins. To evaluate the influence of the sugar and/or protein feeding over time, samples were collected during six consecutive harvests. (13)C isotopic ratio measurements of natural RJs gave values of around -25 ‰, which were also seen for RJs obtained when the bees were fed honey or C3 sugars. However, the RJs obtained when the bees were fed cane sugar or corn hydrolysate (regardless of whether they were also fed proteins) gave values of up to -17 ‰. Sugar content analysis revealed that the composition of maltose, maltotriose, sucrose, and erlose varied significantly over time in accordance with the composition of the syrup fed to the bees. When corn and cereal starch hydrolysates were fed to the bees, the maltose and maltotriose contents of the RJs increased up to 5.0 and 1.3 %, respectively, compared to the levels seen in authentic samples (i.e., samples obtained when the bees were fed natural food: honey and pollen) that were inferior to 0.2% and not detected, respectively. The sucrose and erlose contents of natural RJs were around 0.2 %, whereas those in RJs obtained when the bees were fed cane or beet sugar were as much as 4.0 and 1.3 %, respectively. The combination of sugar analysis and (13)C isotopic ratio measurements represents a very efficient analytical methodology for detecting (from early harvests onward) the use of C4 and C3 artificial sugars in the production of RJ.

Effect of Plant Age on the Quantity and Quality of Proteins Extracted from Sugar Beet (Beta vulgaris L.) Leaves.

PubMed

Kiskini, Alexandra; Vissers, Anne; Vincken, Jean-Paul; Gruppen, Harry; Wierenga, Peter Alexander

2016-11-09

Effects of the developmental stage (e.g., young, mature, or senescent) of leaves on their chemical composition have been described in the literature. This study focuses on the variation in chemical composition and quantity and quality of proteins extracted from leaves due to variation in plant age (i.e., harvesting time), using leaves from sugar beets grown in a field (Rhino, Arrival) and in a greenhouse (Isabella). Within the same variety (Rhino, field; Arrival, field; Isabella, greenhouse) the protein content was similar for leaves from young and old plants (22 ± 1, 16 ± 1, and 10 ± 3% w/w db, respectively). Variation in final protein isolation yield was mostly due to variation in nitrogen extractability (28-56%), although no consistent correlation with plant age was found. A significant effect of plant age was observed on the quality (color) of the extracted protein, that is, brown (indicative of polyphenol oxidase activity) and yellow for extracts from old and young plants, respectively.

21 CFR 73.40 - Dehydrated beets (beet powder).

Code of Federal Regulations, 2013 CFR

2013-04-01

... LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.40 Dehydrated beets (beet powder). (a... mixtures for coloring foods. (b) Specifications. The color additive shall conform to the following... that it may not be used to color foods for which standards of identity have been promulgated under...

21 CFR 73.40 - Dehydrated beets (beet powder).

Code of Federal Regulations, 2014 CFR

2014-04-01

... LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.40 Dehydrated beets (beet powder). (a... mixtures for coloring foods. (b) Specifications. The color additive shall conform to the following... that it may not be used to color foods for which standards of identity have been promulgated under...

21 CFR 73.40 - Dehydrated beets (beet powder).

Code of Federal Regulations, 2012 CFR

2012-04-01

... LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.40 Dehydrated beets (beet powder). (a... mixtures for coloring foods. (b) Specifications. The color additive shall conform to the following... that it may not be used to color foods for which standards of identity have been promulgated under...

21 CFR 73.40 - Dehydrated beets (beet powder).

Code of Federal Regulations, 2011 CFR

2011-04-01

... mixtures for coloring foods. (b) Specifications. The color additive shall conform to the following... used for the coloring of foods generally in amounts consistent with good manufacturing practice, except... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Dehydrated beets (beet powder). 73.40 Section 73...

78 FR 36508 - Notice of Sugar Purchase and Exchange for Re-Export Program Credits; and Notice of Re-Export...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-18

... DEPARTMENT OF AGRICULTURE Commodity Credit Corporation Office of the Secretary Notice of Sugar... to purchase sugar to be offered in exchange for Refined Sugar Re-export Program credits. CCC will purchase sugar from domestic sugarcane processors or beet processors under the Cost Reduction Options of...

Strain-specific colonization pattern of Rhizoctonia antagonists in the root system of sugar beet.

PubMed

Zachow, Christin; Fatehi, Jamshid; Cardinale, Massimiliano; Tilcher, Ralf; Berg, Gabriele

2010-10-01

To develop effective biocontrol strategies, basic knowledge of plant growth promotion (PGP) and root colonization by antagonists is essential. The survival and colonization patterns of five different biocontrol agents against Rhizoctonia solani AG2-2IIIB in the rhizosphere of greenhouse-grown sugar beet plants were analysed in single and combined treatments. The study included bacteria (Pseudomonas fluorescens L13-6-12, Pseudomonas trivialis RE(*) 1-1-14, Serratia plymuthica 3Re4-18) as well as fungi (Trichoderma gamsii AT1-2-4, Trichoderma velutinum G1/8). Microscopic analysis by confocal laser scanning microscopy revealed different colonization patterns for each DsRed2/green fluorescent protein-labelled strain. Bacteria and T. velutinum G1/8 colonized the root surface and the endorhiza in single and co-culture, while for T. gamsii AT1-2-4, only the transfer of spores was observed. Whereas Pseudomonas strains formed large microcolonies consisting of hundreds of cells, S. plymuthica was arranged in small endophytic clusters or clouds around the entire root system. In co-culture, each strain showed its typical pattern and occupied specific niches on the root, without clear evidence of morphological interactions. PGP was only observed for four strains with rhizosphere competence and not for T. gamsii AT1-2-4. The results provide useful information on which combination of strains to test in larger biocontrol experiments directed to applications. © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Roles of Reversible and Irreversible Aggregation in Sugar Processing

USDA-ARS?s Scientific Manuscript database

Colloids (1-1000 nm particles) in sugar cane/beet juice originate from non-sucrose impurities (polyphenolic colorants, residual soil, polysaccharides) of the plant materials; additional colloids form during the high temperature processing. Colloids are reactive towards aggregation, sorption, desorp...

Combined HILIC-ELSD/ESI-MS(n) enables the separation, identification and quantification of sugar beet pectin derived oligomers.

PubMed

Remoroza, C; Cord-Landwehr, S; Leijdekkers, A G M; Moerschbacher, B M; Schols, H A; Gruppen, H

2012-09-01

The combined action of endo-polygalacturonase (endo-PGII), pectin lyase (PL), pectin methyl esterase (fungal PME) and RG-I degrading enzymes enabled the extended degradation of methylesterified and acetylated sugar beet pectins (SBPs). The released oligomers were separated, identified and quantified using hydrophilic interaction liquid chromatography (HILIC) with online electrospray ionization ion trap mass spectrometry (ESI-IT-MS(n)) and evaporative light scattering detection (ELSD). By MS(n), the structures of galacturonic acid (GalA) oligomers having an acetyl group in the O-2 and/or O-3 positions eluting from the HILIC column were elucidated. The presence of methylesterified and/or acetylated galacturonic acid units within an oligomer reduced the interaction with the HILIC column significantly compared to the unsubstituted GalA oligomers. The HILIC column enables a good separation of most oligomers present in the digest. The use of ELSD to quantify oligogalacturonides was validated using pure GalA standards and the signal was found to be independent of the chemical structure of the oligomer being detected. The combination of chromatographic and enzymatic strategies enables to distinguish SBPs having different methylesters and acetyl group distribution. Copyright © 2012 Elsevier Ltd. All rights reserved.

Beet juice utilization: Expeditious green synthesis of nobel metal nanoparticles (Ag, Au, Pt, and Pd) using microwaves

EPA Science Inventory

Metal nanoparticles of Ag, Au, Pt, and Pd were prepared in aqueous solutions via a rapid microwave-assisted green method using beet juice, an abundant sugar-rich agricultural produce, served as both a reducing and a capping reagent. The Ag nanoparticles with capping prepared by b...

Impact of 2,4-Diacetylphloroglucinol-Producing Biocontrol Strain Pseudomonas fluorescens F113 on Intraspecific Diversity of Resident Culturable Fluorescent Pseudomonads Associated with the Roots of Field-Grown Sugar Beet Seedlings

PubMed Central

Moënne-Loccoz, Yvan; Tichy, Hans-Volker; O'Donnell, Anne; Simon, Reinhard; O'Gara, Fergal

2001-01-01

The impact of the 2,4-diacetylphloroglucinol-producing biocontrol agent Pseudomonas fluorescens F113Rif on the diversity of the resident community of culturable fluorescent pseudomonads associated with the roots of field-grown sugar beet seedlings was evaluated. At 19 days after sowing, the seed inoculant F113Rif had replaced some of the resident culturable fluorescent pseudomonads at the rhizoplane but had no effect on the number of these bacteria in the rhizosphere. A total of 498 isolates of resident fluorescent pseudomonads were obtained and characterized by molecular means at the level of broad phylogenetic groups (by amplified ribosomal DNA restriction analysis) and at the strain level (with random amplified polymorphic DNA markers) as well as phenotypically (55 physiological tests). The introduced pseudomonad induced a major shift in the composition of the resident culturable fluorescent Pseudomonas community, as the percentage of rhizoplane isolates capable of growing on three carbon substrates (erythritol, adonitol, and l-tryptophan) not assimilated by the inoculant was increased from less than 10% to more than 40%. However, the pseudomonads selected did not display enhanced resistance to 2,4-diacetylphloroglucinol. The shift in the resident populations, which was spatially limited to the surface of the root (i.e., the rhizoplane), took place without affecting the relative proportions of phylogenetic groups or the high level of strain diversity of the resident culturable fluorescent Pseudomonas community. These results suggest that the root-associated Pseudomonas community of sugar beet seedlings is resilient to the perturbation that may be caused by a taxonomically related inoculant. PMID:11472913

Population Dynamics of Dactylella oviparasitica and Heterodera schachtii: Toward a Decision Model for Sugar Beet Planting

PubMed Central

Yang, Jiue-in; Benecke, Scott; Jeske, Daniel R.; Rocha, Fernando S.; Smith Becker, Jennifer; Timper, Patricia; Ole Becker, J.

2012-01-01

A series of experiments were performed to examine the population dynamics of the sugarbeet cyst nematode, Heterodera schachtii, and the nematophagus fungus Dactylella oviparasitica. After two nematode generations, the population densities of H. schachtii were measured in relation to various initial infestation densities of both D. oviparasitica and H. schachtii. In general, higher initial population densities of D. oviparasitica were associated with lower final population densities of H. schachtii. Regression models showed that the initial densities of D. oviparasitica were only significant when predicting the final densities of H. schachtii J2 and eggs as well as fungal egg parasitism, while the initial densities of J2 were significant for all final H. schachtii population density measurements. We also showed that the densities of H. schachtii-associated D. oviparasitica fluctuate greatly, with rRNA gene numbers going from zero in most field-soil-collected cysts to an average of 4.24 x 108 in mature females isolated directly from root surfaces. Finally, phylogenetic analysis of rRNA genes suggested that D. oviparasitica belongs to a clade of nematophagous fungi that includes Arkansas Fungus strain L (ARF-L) and that these fungi are widely distributed. We anticipate that these findings will provide foundational data facilitating the development of more effective decision models for sugar beet planting. PMID:23481664

Targeted next-generation sequencing identification of mutations in disease resistance gene anologs (RGAs) in wild and cultivated beets

USDA-ARS?s Scientific Manuscript database

Resistance gene analogs (RGAs) were searched bioinformatically in the sugar beet (Beta vulgaris L.) genome as potential candidates for improving resistance against different diseases. In the present study, Ion Torrent sequencing technology was used to identify mutations in 21 RGAs. The DNA samples o...

Tillage as a tool to manage crop residue: impact on sugar beet production.

NASA Astrophysics Data System (ADS)

Hiel, Marie-Pierre; Chélin, Marie; Degrune, Florine; Parvin, Nargish; Bodson, Bernard

2015-04-01

Crop residues and plant cover represent a pool of organic matter that can be used either to restore organic matter in soils, and therefore maintain soil fertility, or that can be valorized outside of the field (e.g. energy production). However, it is crucial that the exportation of residues is not done to the detriment of the system sustainability. Three long term experiments have been settled in the loamy region in Belgium. All of them are designed to study the effect of residues management by several tillage systems (conventional plowing versus reduced tillage) on the whole soil-water-plant system. SOLRESIDUS is a field experiment where we study the impact of crop residue management while in SOLCOUVERT and SOLCOUVERT-BIS, we study the impact of cover crop management. SOLRESIDUS was started in 2008. In this field, four contrasted crop residues managements are tested in order to contrast as much as possible the responses from the soil-water plant system. Two practices characterize the four modalities: soil tillage (ploughing at 25 cm depth or reduce tillage at 10 cm max) and residue management (exportation or restitution). SOLCOUVERT and SOLCOUVERT-BIS were started in 2012 and 2013 respectively. In those fields cover crop management is also diverse: destruction of the cover crop by winter ploughing, spring ploughing, strip tillage (with a chemical destruction if needed) or shallow tillage (with a decompaction before cover crop sowing). Although although the overall project aims at studying the impact of management on the whole soil-water-plant system, here we will only present the results concerning crop production (sugar beet) in SOLCOUVERT experiments. The presented data will include germination rate, crop development (biomass quantification and BBCH stages) weeds population, disease occurrence, pest occurrences, nitrogen uptake by plants, quality and quantity of harvested products.

76 FR 64839 - Sugar Program; Feedstock Flexibility Program for Bioenergy Producers

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-19

... increase and that high fructose corn syrup (HFCS) use in Mexico continues to be strong (but not as strong..., in- process sugar products such as beet thick juice or cane syrup are eligible. Since the program...

Demand-driven biogas production from sugar beet silage in a novel fixed bed disc reactor under mesophilic and thermophilic conditions.

PubMed

Terboven, Christiane; Ramm, Patrice; Herrmann, Christiane

2017-10-01

A newly developed fixed bed disc reactor (FBDR) which combines biofilm formation on biofilm carriers and reactor agitation in one single system was assessed for its applicability to demand-driven biogas production by variable feeding of sugar beet silage. Five different feeding patterns were studied at an organic loading of 4g VS L -1 d -1 under mesophilic and thermophilic conditions. High methane yields of 449-462L N kg VS were reached. Feeding variable punctual loadings caused immediate response with 1.2- to 3.5-fold increase in biogas production rates within 15min. Although variable feeding did not induce process instability, a temporary decrease in pH-value and methane concentration below 40% occurred. Thermophilic temperature was advantageous as it resulted in a more rapid, higher methane production and less pronounced decrease in methane content after feeding. The FBDR was demonstrated to be well-suited for flexible biogas production, but further research and comparison with traditional reactor systems are required. Copyright © 2017 Elsevier Ltd. All rights reserved.

Response surface optimization of the thermal acid pretreatment of sugar beet pulp for bioethanol production using Trichoderma viride and Saccharomyces cerevisiae.

PubMed

El-Gendy, Nour Sh; Madian, Hekmat R; Nassar, Hussein N; Abu Amr, Salem S

2015-01-01

Worldwide nowadays, relying on the second generation bioethanol from the lignocellulosic feedstock is a mandatory aim. However, one of the major drawbacks for high ethanol yield is the physical and chemical pretreatment of this kind of feedstock. As the pretreatment is a crucial process operation that modifies the lignocellulosic structure and enhances its accessibility for the high cost hydrolytic enzymes in an attempt to maximize the yield of the fermentable sugars. The objective of this work was to optimize and integrate a physicochemical pretreatment of one of the major agricultural wastes in Egypt; the sugar beet pulp (SBP) and the enzymatic saccharification of the pretreated SBP using a whole fungal cells with a separate bioethanol fermentation batch processes to maximize the bioethanol yield. The response surface methodology was employed in this study to statistically evaluate and optimize the conditions for a thermal acid pretreatment of SBP. The significance and the interaction effects of the concentrations of HCl and SBP and the reaction temperature and time were studied using a three-level central composite design of experiments. A quadratic model equation was obtained to maximize the production of the total reducing sugars. The validity of the predicted model was confirmed. The thermally acid pretreated SBP was further subjected to a solid state fermentation batch process using Trichoderma viride F94. The thermal acid pretreatment and fungal hydrolyzes were integrated with two parallel batch fermentation processes of the produced hydrolyzates using Saccharomyces cerevisiae Y39, that yielded a total of ≈ 48 g/L bioethanol, at a conversion rate of ≈ 0.32 g bioethanol/ g SBP. Applying the proposed integrated process, approximately 97.5 gallon of ethanol would be produced from a ton (dry weight) of SBP.

Response surface optimization of the thermal acid pretreatment of sugar beet pulp for bioethanol production using Trichoderma viride and Saccharomyces cerevisiae.

PubMed

El-Gendy, Nour Sh; Madian, Hekmat R; Nassar, Hussein N; Amr, Salem S Abu

2015-09-15

Worldwide nowadays, relying on the second generation bioethanol from the lignocellulosic feedstock is a mandatory aim. However, one of the major drawbacks for high ethanol yield is the physical and chemical pretreatment of this kind of feedstock. As the pretreatment is a crucial process operation that modifies the lignocellulosic structure and enhances its accessibility for the high cost hydrolytic enzymes in an attempt to maximize the yield of the fermentable sugars. The objective of this work was to optimize and integrate a physicochemical pretreatment of one of the major agricultural wastes in Egypt; the sugar beet pulp (SBP) and the enzymatic saccharification of the pretreated SBP using a whole fungal cells with a separate bioethanol fermentation batch processes to maximize the bioethanol yield. The response surface methodology was employed in this study to statistically evaluate and optimize the conditions for a thermal acid pretreatment of SBP. The significance and the interaction effects of the concentrations of HCl and SBP and the reaction temperature and time were studied using a three-level central composite design of experiments. A quadratic model equation was obtained to maximize the production of the total reducing sugars. The validity of the predicted model was confirmed. The thermally acid pretreated SBP was further subjected to a solid state fermentation batch process using Trichoderma viride F94. The thermal acid pretreatment and fungal hydrolyzes were integrated with two parallel batch fermentation processes of the produced hydrolyzates using Saccharomyces cerevisiae Y39, that yielded a total of ≈ 48 g/L bioethanol, at a conversion rate of ≈ 0.32 g bioethanol/ g SBP. Applying the proposed integrated process, approximately 97.5 gallon of ethanol would be produced from a ton (dry weight) of SBP.

Semi-continuous anaerobic co-digestion of sugar beet byproduct and pig manure: Effect of the organic loading rate (OLR) on process performance.

PubMed

Aboudi, Kaoutar; Álvarez-Gallego, Carlos José; Romero-García, Luis Isidoro

2015-10-01

Anaerobic co-digestion of dried pellet of exhausted sugar beet cossettes (ESBC-DP) with pig manure (PM) was investigated in a semi-continuous stirred tank reactor (SSTR) under mesophilic conditions. Seven hydraulic retention times (HRT) from 20 to 5 days were tested with the aim to evaluate the methane productivities and volatile solids (VS) removal. The corresponding organic loading rates (OLR) ranged from 4.2 to 12.8 gVS/L(reactor) d. The findings revealed that highest system efficiency was achieved at an OLR of 11.2 gVS/L(reactor) d (6 days-HRT) with a methane production rate (MPR) and volatile solids (VS) reduction of 2.91 LCH4/L(reactor) d and 57.5%, respectively. The HRT of 5 days was found critical for the studied process, which leads to volatile fatty acids (VFA) accumulation and sharp drop in pH. However, the increase of HRT permits the recovery of system. Copyright © 2015 Elsevier Ltd. All rights reserved.

Enzymatic Browning in Sugar Beet Leaves (Beta vulgaris L.): Influence of Caffeic Acid Derivatives, Oxidative Coupling, and Coupled Oxidation.

PubMed

Vissers, Anne; Kiskini, Alexandra; Hilgers, Roelant; Marinea, Marina; Wierenga, Peter Alexander; Gruppen, Harry; Vincken, Jean-Paul

2017-06-21

Sugar beet (Beta vulgaris L.) leaves of 8 month (8 m ) plants showed more enzymatic browning than those of 3 month (3 m ). Total phenolic content increased from 4.6 to 9.4 mg/g FW in 3 m and 8 m , respectively, quantitated by reverse-phase-ultrahigh-performance liquid chromatography-ultraviolet-mass spectrometry (RP-UHPLC-UV-MS). The PPO activity was 6.7 times higher in extracts from 8 m than from 3 m leaves. Substrate content increased from 0.53 to 2.45 mg/g FW in 3 m and 8 m , respectively, of which caffeic acid glycosyl esters were most important, increasing 10-fold with age. Caffeic acid glycosides and vitexin derivatives were no substrates. In 3 m and 8 m, nonsubstrate-to-substrate ratios were 8:1 and 3:1, respectively. A model system showed browning at 3:1 ratio due to formation of products with extensive conjugated systems through oxidative coupling and coupled oxidation. The 8:1 ratio did not turn brown as oxidative coupling occurred without much coupled oxidation. We postulate that differences in nonsubstrate-to-substrate ratio and therewith extent of coupled oxidation explain browning.

Enzymatic Browning in Sugar Beet Leaves (Beta vulgaris L.): Influence of Caffeic Acid Derivatives, Oxidative Coupling, and Coupled Oxidation

PubMed Central

2017-01-01

Sugar beet (Beta vulgaris L.) leaves of 8 month (8m) plants showed more enzymatic browning than those of 3 month (3m). Total phenolic content increased from 4.6 to 9.4 mg/g FW in 3m and 8m, respectively, quantitated by reverse-phase-ultrahigh-performance liquid chromatography–ultraviolet-mass spectrometry (RP-UHPLC–UV-MS). The PPO activity was 6.7 times higher in extracts from 8m than from 3m leaves. Substrate content increased from 0.53 to 2.45 mg/g FW in 3m and 8m, respectively, of which caffeic acid glycosyl esters were most important, increasing 10-fold with age. Caffeic acid glycosides and vitexin derivatives were no substrates. In 3m and 8m, nonsubstrate-to-substrate ratios were 8:1 and 3:1, respectively. A model system showed browning at 3:1 ratio due to formation of products with extensive conjugated systems through oxidative coupling and coupled oxidation. The 8:1 ratio did not turn brown as oxidative coupling occurred without much coupled oxidation. We postulate that differences in nonsubstrate-to-substrate ratio and therewith extent of coupled oxidation explain browning. PMID:28570816

Multiple applications of ion chromatography oligosaccharide fingerprint profiles to solve a variety of sugar and sugar-biofuel industry problems.

PubMed

Eggleston, Gillian; Borges, Eduardo

2015-03-25

Sugar crops contain a broad variety of carbohydrates used for human consumption and the production of biofuels and bioproducts. Ion chromatography with integrated pulsed amperometric detection (IC-IPAD) can be used to simultaneously detect mono-, di-, and oligosaccharides, oligosaccharide isomers, mannitol, and ethanol in complex matrices from sugar crops. By utilizing a strong NaOH/NaOAc gradient method over 45 min, oligosaccharides of at least 2-12 dp can be detected. Fingerprint IC oligosaccharide profiles are extremely selective, sensitive, and reliable and can detect deterioration product metabolites from as low as 100 colony-forming units/mL lactic acid bacteria. The IC fingerprints can also be used to (i) monitor freeze deterioration, (ii) optimize harvesting methods and cut-to-crush times, (iii) differentiate between white refined sugar from sugar cane and from sugar beets, (iv) verify the activities of carbohydrate enzymes, (v) select yeasts for ethanol fermentations, and (vi) isolate and diagnose infections and processing problems in sugar factories.

Effect of interfacial composition and crumbliness on aroma release in soy protein/sugar beet pectin mixed emulsion gels.

PubMed

Hou, Jun-Jie; Guo, Jian; Wang, Jin-Mei; Yang, Xiao-Quan

2016-10-01

In this study, soy protein isolate/sugar beet pectin (SPI/SBP) emulsion gels were prepared through an enzymatic gelation process. The effects of emulsifier (SBP, SPI or SPI/SBP complex) and emulsification process on the microstructure, texture, breakdown properties and aroma release behavior of resulting emulsion gels were investigated. Oil emulsification by SBP/SPI complex resulted in a higher amount of emulsifier absorbing on the oil-water interface than by SBP and SPI alone, indicating that a more compact interfacial network was formed. Flocculation of oil droplets was observed and corresponding emulsion gels exhibited lower fracture force and strain when the oil was emulsified by SPI and SBP/SPI complex. Moreover, emulsion gels with small droplets produced a greater quantity of small fragments after mastication. However, microstructure did not have a significant effect on breakdown properties of emulsion gels. Headspace gas chromatography analysis showed that the release rate of ethyl butyrate before and after mastication was significantly lower in emulsion gel with more compact network, but the release of aroma compounds with higher hydrophobicity did not show a significant influence of the microstructure and texture of emulsion gel. This finding provides a useful application for designing semi-solid foods with desirable flavor perception. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Drought Impact Is Alleviated in Sugar Beets (Beta vulgaris L.) by Foliar Application of Fullerenol Nanoparticles

PubMed Central

Borišev, Milan; Borišev, Ivana; Župunski, Milan; Arsenov, Danijela; Pajević, Slobodanka; Ćurčić, Živko; Vasin, Jovica; Djordjevic, Aleksandar

2016-01-01

Over the past few years, significant efforts have been made to decrease the effects of drought stress on plant productivity and quality. We propose that fullerenol nanoparticles (FNPs, molecular formula C60(OH)24) may help alleviate drought stress by serving as an additional intercellular water supply. Specifically, FNPs are able to penetrate plant leaf and root tissues, where they bind water in various cell compartments. This hydroscopic activity suggests that FNPs could be beneficial in plants. The aim of the present study was to analyse the influence of FNPs on sugar beet plants exposed to drought stress. Our results indicate that intracellular water metabolism can be modified by foliar application of FNPs in drought exposed plants. Drought stress induced a significant increase in the compatible osmolyte proline in both the leaves and roots of control plants, but not in FNP treated plants. These results indicate that FNPs could act as intracellular binders of water, creating an additional water reserve, and enabling adaptation to drought stress. Moreover, analysis of plant antioxidant enzyme activities (CAT, APx and GPx), MDA and GSH content indicate that fullerenol foliar application could have some beneficial effect on alleviating oxidative effects of drought stress, depending on the concentration of nanoparticles applied. Although further studies are necessary to elucidate the biochemical impact of FNPs on plants; the present results could directly impact agricultural practice, where available water supplies are often a limiting factor in plant bioproductivity. PMID:27832171

Two-stage anaerobic digestion of sugar beet silage: The effect of the pH-value on process parameters and process efficiency.

PubMed

Kumanowska, Elzbieta; Uruñuela Saldaña, Mariana; Zielonka, Simon; Oechsner, Hans

2017-12-01

The study investigated the influence of the target pH-values 4.5, 5, 5.5 and 6 in the acidification reactor on process parameters, such as substrate-specific methane yield and the intermediates, in the two-stage anaerobic digestion of sugar beet silage. The total specific methane yield (Nlkg -1 CODd -1 ) increased with an increase in the pH (pH 4.5: 140.58±70.08, pH 5: 181.21±55.71, pH 5.5: 218.32±51.01, pH 6: 256.47±28.78). The pH-value also had an effect on the dominant intermediate in hydrolysate. At the pH-value of 4.5, almost no acidification and microbial activity was observed. At pH 5 and 5.5, butyric acid production dominated, guided by H 2 production. At pH 6 acetic acid was the main product. The absence of H 2 and the highest SMY makes it favorable under practical aspects. Copyright © 2017 Elsevier Ltd. All rights reserved.

Interaction between beet vinasse and iron fertilisers in the prevention of iron deficiency in lupins.

PubMed

de Santiago, Ana; Delgado, Antonio

2010-10-01

Recycling of organic byproducts for use as soil amendments or fertilisers may enhance the productivity of soils. The aim of this study was to investigate the potential of sugar beet vinasse to correct iron chlorosis in crops when applied in conjunction with Fe fertilisers such as vivianite and ferrous sulfate (FS). An experiment involving two factors (Fe source and dialysed sugar beet vinasse (DBV) rate) was performed using white lupin (Lupinus albus L.) and calcareous sand as growing medium. Although vivianite provided lower chlorophyll contents than Fe-chelate, dry matter production was not significantly different between the two Fe sources. Vivianite was more effective than FS in preventing iron chlorosis in white lupin, but not when DBV was applied. DBV significantly increased chlorophyll content in plants treated with FS after 3 weeks of growth. DBV increased the effect of FS in preventing iron deficiency chlorosis in white lupin. This was due, at least in part, to the inhibition of the precipitation of Fe oxides by organic compounds and to the increase in the content of Fe complexed by organic compounds in the growing medium, as revealed by sequential Fe fractionation. Copyright © 2010 Society of Chemical Industry.

Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus.

PubMed

Almasi, Mohammad Amin; Almasi, Galavizh

2017-02-01

Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.

Effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production.

PubMed

El-Tayeb, T S; Abdelhafez, A A; Ali, S H; Ramadan, E M

2012-10-01

This study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. The chemical composition of these feedstocks was determined. Conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. In single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the conversion percentage of almost all treated agro-industrial wastes. Lower conversion percentages for some treatments were obtained when increasing the residence time from 90 to 120 min. The two-stage dilute acid hydrolysis by phosphoric acid (1.0 % v/v) followed by sulphuric acid (1.0 % v/v) resulted in the highest conversion percentage (41.3 % w/w) on treated sugar beet waste. This treatment when neutralized, amended with some nutrients and inoculated with baker's yeast, achieved the highest ethanol concentration (1.0 % v/v). Formation of furfural and hydroxymethylfurfural (HMF) were functions of type of acid hydrolysis, acid concentration, residence time and feedstock type. The highest bioconversion of 5 % wastes (37.8 % w/w) was recorded on sugar beet waste by Trichoderma viride EMCC 107. This treatment when followed by baker's yeast fermentation, 0.41 % (v/v) ethanol and 8.2 % (v/w) conversion coefficient were obtained.

Effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production

PubMed Central

El-Tayeb, T.S.; Abdelhafez, A.A.; Ali, S.H.; Ramadan, E.M.

2012-01-01

This study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. The chemical composition of these feedstocks was determined. Conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. In single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the conversion percentage of almost all treated agro-industrial wastes. Lower conversion percentages for some treatments were obtained when increasing the residence time from 90 to 120 min. The two-stage dilute acid hydrolysis by phosphoric acid (1.0 % v/v) followed by sulphuric acid (1.0 % v/v) resulted in the highest conversion percentage (41.3 % w/w) on treated sugar beet waste. This treatment when neutralized, amended with some nutrients and inoculated with baker’s yeast, achieved the highest ethanol concentration (1.0 % v/v). Formation of furfural and hydroxymethylfurfural (HMF) were functions of type of acid hydrolysis, acid concentration, residence time and feedstock type. The highest bioconversion of 5 % wastes (37.8 % w/w) was recorded on sugar beet waste by Trichoderma viride EMCC 107. This treatment when followed by baker’s yeast fermentation, 0.41 % (v/v) ethanol and 8.2 % (v/w) conversion coefficient were obtained. PMID:24031984

Transcriptome Analysis of Beta macrocarpa and Identification of Differentially Expressed Transcripts in Response to Beet Necrotic Yellow Vein Virus Infection.

PubMed

Fan, Huiyan; Zhang, Yongliang; Sun, Haiwen; Liu, Junying; Wang, Ying; Wang, Xianbing; Li, Dawei; Yu, Jialin; Han, Chenggui

2015-01-01

Rhizomania is one of the most devastating diseases of sugar beet. It is caused by Beet necrotic yellow vein virus (BNYVV) transmitted by the obligate root-infecting parasite Polymyxa betae. Beta macrocarpa, a wild beet species widely used as a systemic host in the laboratory, can be rub-inoculated with BNYVV to avoid variation associated with the presence of the vector P. betae. To better understand disease and resistance between beets and BNYVV, we characterized the transcriptome of B. macrocarpa and analyzed global gene expression of B. macrocarpa in response to BNYVV infection using the Illumina sequencing platform. The overall de novo assembly of cDNA sequence data generated 75,917 unigenes, with an average length of 1054 bp. Based on a BLASTX search (E-value ≤ 10-5) against the non-redundant (NR, NCBI) protein, Swiss-Prot, the Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, there were 39,372 unigenes annotated. In addition, 4,834 simple sequence repeats (SSRs) were also predicted, which could serve as a foundation for various applications in beet breeding. Furthermore, comparative analysis of the two transcriptomes revealed that 261 genes were differentially expressed in infected compared to control plants, including 128 up- and 133 down-regulated genes. GO analysis showed that the changes in the differently expressed genes were mainly enrichment in response to biotic stimulus and primary metabolic process. Our results not only provide a rich genomic resource for beets, but also benefit research into the molecular mechanisms of beet- BNYV Vinteraction.

The anaerobic digestion of pig carcase with or without sugar beet pulp, as a novel on-farm disposal method.

PubMed

Kirby, Marie E; Theodorou, Michael K; Brizuela, Carole M; Huntington, James A; Powles, Jayne; Wilkinson, Robert G

2018-05-01

Anaerobic digestion was investigated as a potential method for on-farm disposal of fallen stock (pig carcases), degrading the carcase material to produce biogas and digestate. The effects of feedstock (sugar beet pulp or pig carcase material or a 50:50 mix) and organic loading rate (50 g-TS L -1 or 100 g-TS L -1 ), during mesophilic (35 °C) anaerobic digestion were investigated. Anaerobic digestion was achieved for all experimental treatments, however the pig carcase material at the higher organic loading rate produced the second highest methane yield (0.56 Nm 3 kg-VS -1 versus a range of 0.14-0.58 Nm 3 kg-VS -1 for other treatments), with the highest percentage of methane in total biogas (61.6% versus a range of 36.1-55.2% for all other treatments). Satisfactory pathogen reduction is a legislative requirement for disposal of carcase material. Pathogens were quantified throughout the anaerobic digestion process. Enterococcus faecalis concentrations decreased to negligible levels (2.8 log 10 CFU g-TS -1 ), whilst Clostridium perfringens levels remained unaffected by treatment throughout the digestion process (5.3 ± 0.2 log 10 CFU g-TS -1 ). Copyright © 2018 Elsevier Ltd. All rights reserved.

Photolabeling of tonoplast from sugar beet cell suspensions by [h]5-(N-methyl-N-isobutyl)-amiloride, an inhibitor of the vacuolar na/h antiport.

PubMed

Barkla, B J; Charuk, J H; Cragoe, E J; Blumwald, E

1990-07-01

The effects of 5-(N-methyl-N-isobutyl)-amiloride (MIA), an amiloride analog, was tested on the Na(+)/H(+) antiport activity of intact vacuoles and tonoplast vesicles isolated from sugar beet (Beta vulgaris L.) cell suspension cultures. MIA inhibited Na(+)/H(+) exchange in a competitive manner with a K(i) of 2.5 and 5.9 micromolar for DeltapH-dependent (22)Na(+) influx in tonoplast vesicles and Na(+)-dependent H(+) efflux in intact vacuoles, respectively. Scatchard analysis of the binding of [(3)H]MIA to tonoplast membranes revealed a high affinity binding component with a K(d) of 1.3 micromolar. The close relationship between the dissociation constant value obtained and the constants of inhibition for MIA obtained by fluorescence quenching and isotope exchange suggests that the high affinity component represents a class of sites associated with the tonoplast Na(+)/H(+) antiport. Photolabeling of the tonoplast with [(3)H]MIA revealed two sets of polypeptides with a different affinity to amiloride and its analog.

Analysis of the resistance-breaking ability of different beet necrotic yellow vein virus isolates loaded into a single Polymyxa betae population in soil.

PubMed

Bornemann, Kathrin; Varrelmann, Mark

2011-06-01

The genome of most Beet necrotic yellow vein virus (BNYVV) isolates is comprised of four RNAs. The ability of certain isolates to overcome Rz1-mediated resistance in sugar beet grown in the United States and Europe is associated with point mutations in the pathogenicity factor P25. When the virus is inoculated mechanically into sugar beet roots at high density, the ability depends on an alanine to valine substitution at P25 position 67. Increased aggressiveness is shown by BNYVV P type isolates, which carry an additional RNA species that encodes a second pathogenicity factor, P26. Direct comparison of aggressive isolates transmitted by the vector, Polymyxa betae, has been impossible due to varying population densities of the vector and other soilborne pathogens that interfere with BNYVV infection. Mechanical root inoculation and subsequent cultivation in soil that carried a virus-free P. betae population was used to load P. betae with three BNYVV isolates: a European A type isolate, an American A type isolate, and a P type isolate. Resistance tests demonstrated that changes in viral aggressiveness towards Rz1 cultivars were independent of the vector population. This method can be applied to the study of the synergism of BNYVV with other P. betae-transmitted viruses.

Influence of the incorporation mode of sugar beet pulp in the finishing diet on the digestive tract and performances of geese reared for foie gras production.

PubMed

Arroyo, J; Lavigne, F; Bannelier, C; Fortun-Lamothe, L

2017-09-01

The aim of this work was to study the effects of incorporating sugar beet pulp (SBP) into the diet of geese in two feeding systems (complete pelleted feed or loose-mix feeding system) on crop development and performance. A total of 480 1-d-old male geese were divided into three groups whose diet differed from d 56 to 90: a complete pelleted diet containing 50% corn (control diet: AMEn 11.5 MJ/kg; CP 161 g/kg), and no SBP; a complete pelleted diet containing 50% corn and 10% SBP (SBPcp diet: AMEn: 11.5 MJ/kg; CP: 161 g/kg); and a mix in the same feeder (SBPlm diet) of 500 g/kg of protein-rich pellets containing 20% SBP (SBPprp: AMEn: 9.0 MJ/kg; CP: 250 g/kg) and 500 g/kg of whole corn (WC: AMEn: 14.0 MJ/kg; CP: 72 g/kg). Body traits, including crop volume, were measured at d 91. From d 91 to 106, 88 birds/group were overfed with a mixture containing mainly corn and water before slaughter to measure fatty liver performance. Feed intake from d 56 to 90 was higher (+10%; P = 0.004) in the SBPcp group than the other two, but at d 90, the body weight (BW) of the birds was higher (+7%; P = 0.002) in the SBPlm group than the other two. At d 91, the volume of the crop was greater in the SBPcp group (80.4 mL/kg of BW, P < 0.001) than in the control group (60.3 mL/kg of BW), the SBPlm group being intermediate (64.1 mL/kg of BW). Feed intake (13,321 g), weight gain (2,733 g), and feed-to-gain ratio (4.9) during the overfeeding period, as well as fatty liver weight (963 g) and commercial grading, were similar (P > 0.05) between the three groups. In conclusion, the use of sugar beet pulp in the diet of finishing geese helps the adaptation of the digestive tract to the overfeeding period, even in a loose-mix feeding system based on whole corn. © 2017 Poultry Science Association Inc.

7 CFR 1435.300 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar... allotments for: (1) Processor marketings of sugar domestically processed from sugar beets or in-process beet sugar, whether such sugar beets or in-process beet sugar were produced domestically or imported, (2...

7 CFR 1435.300 - Applicability.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar... allotments for: (1) Processor marketings of sugar domestically processed from sugar beets or in-process beet sugar, whether such sugar beets or in-process beet sugar were produced domestically or imported, (2...

7 CFR 1435.300 - Applicability.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar... allotments for: (1) Processor marketings of sugar domestically processed from sugar beets or in-process beet sugar, whether such sugar beets or in-process beet sugar were produced domestically or imported, (2...

7 CFR 1435.300 - Applicability.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar... allotments for: (1) Processor marketings of sugar domestically processed from sugar beets or in-process beet sugar, whether such sugar beets or in-process beet sugar were produced domestically or imported, (2...

7 CFR 1435.300 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar... allotments for: (1) Processor marketings of sugar domestically processed from sugar beets or in-process beet sugar, whether such sugar beets or in-process beet sugar were produced domestically or imported, (2...

In Vitro Development from Leaf Explants of Sugar Beet (Beta vulgaris L). Rhizogenesis and the Effect of Sequential Exposure to Auxin and Cytokinin

PubMed Central

Gürel, Ekrem; Wren, M. Jill

1995-01-01

Adventitious root development in lamina and midrib-petiole junction expiants of sugar beet cv. Primo was investigated using scanning electron microscopy and light microscopy. Primordia developed close to the vascular strands and areas of newly dividing cells (meristematic centres) were seen adjacent to the intrafascicular cambium after 2 d incubation on medium containing 30 mg 1−11-naphthalene acetic acid. Clearly defined primordia were visible at 4 d and the first roots had emerged by 6 d. A minimum of 24 h exposure to NAA was necessary for root induction. Four days on NAA caused twice as many roots to be initiated but more prolonged exposure (5 and 10 d) inhibited root development. Root initiation continued after transfer to medium containing no plant growth regulators, new primordia appearing as the older ones extended as roots. Attempts were made to modify the development of primordia by sequential culture on cytokinin after induction by auxin. Incubation on N6-benzylaminopurine within 48 h of exposure to NAA disrupted the development of primordia and roots but did not induce shoot formation. PMID:21247910

Bioethanol production from fermentable sugar juice.

PubMed

Zabed, Hossain; Faruq, Golam; Sahu, Jaya Narayan; Azirun, Mohd Sofian; Hashim, Rosli; Boyce, Amru Nasrulhaq

2014-01-01

Bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. Mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. This paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarcane, sugar beet, and sweet sorghum that are the most attractive choice because of their cost-effectiveness and feasibility to use. Three types of fermentation process (batch, fed-batch, and continuous) are employed in ethanol production from these sugar juices. The most common microorganism used in fermentation from its history is the yeast, especially, Saccharomyces cerevisiae, though the bacterial species Zymomonas mobilis is also potentially used nowadays for this purpose. A number of factors related to the fermentation greatly influences the process and their optimization is the key point for efficient ethanol production from these feedstocks.

Bioethanol Production from Fermentable Sugar Juice

PubMed Central

Zabed, Hossain; Faruq, Golam; Sahu, Jaya Narayan; Azirun, Mohd Sofian; Hashim, Rosli; Nasrulhaq Boyce, Amru

2014-01-01

Bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. Mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. This paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarcane, sugar beet, and sweet sorghum that are the most attractive choice because of their cost-effectiveness and feasibility to use. Three types of fermentation process (batch, fed-batch, and continuous) are employed in ethanol production from these sugar juices. The most common microorganism used in fermentation from its history is the yeast, especially, Saccharomyces cerevisiae, though the bacterial species Zymomonas mobilis is also potentially used nowadays for this purpose. A number of factors related to the fermentation greatly influences the process and their optimization is the key point for efficient ethanol production from these feedstocks. PMID:24715820

Functionally redundant but dissimilar microbial communities within biogas reactors treating maize silage in co-fermentation with sugar beet silage

PubMed Central

Langer, Susanne G; Ahmed, Sharif; Einfalt, Daniel; Bengelsdorf, Frank R; Kazda, Marian

2015-01-01

Numerous observations indicate a high flexibility of microbial communities in different biogas reactors during anaerobic digestion. Here, we describe the functional redundancy and structural changes of involved microbial communities in four lab-scale continuously stirred tank reactors (CSTRs, 39°C, 12 L volume) supplied with different mixtures of maize silage (MS) and sugar beet silage (SBS) over 80 days. Continuously stirred tank reactors were fed with mixtures of MS and SBS in volatile solid ratios of 1:0 (Continuous Fermenter (CF) 1), 6:1 (CF2), 3:1 (CF3), 1:3 (CF4) with equal organic loading rates (OLR 1.25 kgVS m−3 d−1) and showed similar biogas production rates in all reactors. The compositions of bacterial and archaeal communities were analysed by 454 amplicon sequencing approach based on 16S rRNA genes. Both bacterial and archaeal communities shifted with increasing amounts of SBS. Especially pronounced were changes in the archaeal composition towards Methanosarcina with increasing proportion of SBS, while Methanosaeta declined simultaneously. Compositional shifts within the microbial communities did not influence the respective biogas production rates indicating that these communities adapted to environmental conditions induced by different feedstock mixtures. The diverse microbial communities optimized their metabolism in a way that ensured efficient biogas production. PMID:26200922

Graded replacement of maize grain with molassed sugar beet pulp modulated ruminal microbial community and fermentation profile in vitro.

PubMed

Münnich, Matthias; Khol-Parisini, Annabella; Klevenhusen, Fenja; Metzler-Zebeli, Barbara U; Zebeli, Qendrim

2018-02-01

Molassed sugar beet pulp (Bp) is a viable alternative to grains in cattle nutrition for reducing human edible energy input. Yet little is known about the effects of high inclusion rates of Bp on rumen microbiota. This study used an in vitro approach and the quantitative polymerase chain reaction technique to establish the effects of a graded replacement of maize grain (MG) by Bp on the ruminal microbial community, fermentation profile and nutrient degradation. Six different amounts of Bp (0-400 g kg -1 ), which replaced MG in the diet, were tested using the in vitro semi-continuous rumen simulation technique. The increased inclusion of Bp resulted in greater dietary content and degradation of neutral detergent fibre (P < 0.01). Further, Bp feeding enhanced (P < 0.01) the abundance of genus Prevotella and shifted (P < 0.01) the short-chain fatty acid patterns in favour of acetate and propionate and at the expense of butyrate. A total replacement of MG with Bp resulted in an increased daily methane production (P < 0.01). Results suggest positive effects of the replacement of MG by Bp especially in terms of stimulating ruminal acetate and propionate fermentation. However, high replacement rates of Bp resulted in lowered utilization of ammonia and higher ruminal methane production. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Elimination of dioxins in milk by dairy cows after the long-term intake of contaminated sugar beet pellets.

PubMed

Piskorska-Pliszczynska, Jadwiga; Maszewski, Sebastian; Mikolajczyk, Szczepan; Pajurek, Marek; Strucinski, Pawel; Olszowy, Marek

2017-05-01

Dried feed materials, such as sugar beet pellets, may be a source of high levels of PCDD/Fs. The studies followed up dioxin congener elimination from contaminated milk (5.02 ± 1.39 pg WHO PCDD/F-PCBs-TEQ g -1 fat) up to 338 days after withdrawal of polluted feed ingredient (4.37 ± 0.25 ng PCDD/F/DL-PCBs-TEQ kg -1 feed). The main objectives of the study were to estimate the length of the time needed to achieve an acceptable PCDD/F milk level as set by European Union law, to track the fate of the PCDD/F congeners, and to assess the potential risk for consumers associated with long-term consumption of contaminated milk. After approximately 200 days, the PCDD/F levels reached ML (2.5 pg WHO-TEQ g -1 fat), then decreased to the action level after more than 300 days (1.75 pg WHO-TEQ g -1 fat) for most of cows. The potential risk of PCDD/F/DL-PCBs intake was characterised by comparing the theoretically calculated weekly and monthly intakes with the toxicological reference values (TRVs). The dioxin intake for the average adult and high-milk consumers did not exceed half of any of the TRVs applied (TWI, PTMI). The consumption of milk by children in the amount recommended by nutritionists (500 ml day -1 ) resulted in an exposure equal to 317% TWI and 275% PTMI.

29 CFR 780.816 - Processing of specific commodities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... processing of sugar beets, sugar-beet molasses, sugarcane, or maple sap is within the exemption. Operations...

29 CFR 780.816 - Processing of specific commodities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... processing of sugar beets, sugar-beet molasses, sugarcane, or maple sap is within the exemption. Operations...

29 CFR 780.816 - Processing of specific commodities.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... processing of sugar beets, sugar-beet molasses, sugarcane, or maple sap is within the exemption. Operations...

29 CFR 780.816 - Processing of specific commodities.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... processing of sugar beets, sugar-beet molasses, sugarcane, or maple sap is within the exemption. Operations...

29 CFR 780.816 - Processing of specific commodities.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... processing of sugar beets, sugar-beet molasses, sugarcane, or maple sap is within the exemption. Operations...

40 CFR 180.408 - Metalaxyl; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances...-(methoxyacetyl)-alanine methyl ester, each expressed as metalaxyl equivalents, in or on the following food... Beet, garden, tops 0.1 Beet, sugar 0.1 Beet, sugar, molasses 1.0 Beet, sugar, roots 0.5 Beet, sugar...

40 CFR 180.408 - Metalaxyl; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances...-(methoxyacetyl)-alanine methyl ester, each expressed as metalaxyl equivalents, in or on the following food... Beet, garden, tops 0.1 Beet, sugar 0.1 Beet, sugar, molasses 1.0 Beet, sugar, roots 0.5 Beet, sugar...

40 CFR 180.408 - Metalaxyl; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances...-(methoxyacetyl)-alanine methyl ester, each expressed as metalaxyl equivalents, in or on the following food... Beet, garden, tops 0.1 Beet, sugar 0.1 Beet, sugar, molasses 1.0 Beet, sugar, roots 0.5 Beet, sugar...

Applying Adaptive Agricultural Management & Industrial Ecology Principles to Produce Lower- Carbon Ethanol from California Energy Beets

NASA Astrophysics Data System (ADS)

Alexiades, Anthy Maria

The life cycle assessment of a proposed beet-to-ethanol pathway demonstrates how agricultural management and industrial ecology principles can be applied to reduce greenhouse gas emissions, minimize agrochemical inputs and waste, provide ecosystem services and yield a lower-carbon fuel from a highly land-use efficient, first-generation feedstock cultivated in California. Beets grown in California have unique potential as a biofuel feedstock. A mature agricultural product with well-developed supply chains, beet-sugar production in California has contracted over recent decades, leaving idle production capacity and forcing growers to seek other crops for use in rotation or find a new market for beets. California's Low Carbon Fuel Standard (LCFS) faces risk of steeply-rising compliance costs, as greenhouse gas reduction targets in the transportation sector were established assuming commercial volumes of lower-carbon fuels from second-generation feedstocks -- such as residues, waste, algae and cellulosic crops -- would be available by 2020. The expected shortfall of cellulosic ethanol has created an immediate need to develop lower-carbon fuels from readily available feedstocks using conventional conversion technologies. The life cycle carbon intensity of this ethanol pathway is less than 28 gCO2e/MJEthanol: a 72% reduction compared to gasoline and 19% lower than the most efficient corn ethanol pathway (34 gCO2e/MJ not including indirect land use change) approved under LCFS. The system relies primarily on waste-to-energy resources; nearly 18 gCO2e/MJ are avoided by using renewable heat and power generated from anaerobic digestion of fermentation stillage and gasification of orchard residues to meet 88% of the facility's steam demand. Co-products displace 2 gCO2e/MJ. Beet cultivation is the largest source of emissions, contributing 15 gCO 2e/MJ. The goal of the study is to explore opportunities to minimize carbon intensity of beet-ethanol and investigate the potential

1H-NMR metabolomic profiling reveals a distinct metabolic recovery response in shoots and roots of temporarily drought-stressed sugar beets

PubMed Central

Maucourt, Mickaël; Deborde, Catherine; Moing, Annick; Gibon, Yves; Goldbach, Heiner E.; Wimmer, Monika A.

2018-01-01

Yield formation in regions with intermittent drought periods depends on the plant’s ability to recover after cessation of the stress. The present work assessed differences in metabolic recovery of leaves and roots of drought-stressed sugar beets with high temporal resolution. Plants were subjected to drought for 13 days, and rewatered for 12 days. At one to two-day intervals, plant material was harvested for untargeted 1H-NMR metabolomic profiling, targeted analyses of hexose-phosphates, starch, amino acids, nitrate and proteins, and physiological measurements including relative water content, osmotic potential, electrolyte leakage and malondialdehyde concentrations. Drought triggered changes in primary metabolism, especially increases in amino acids in both organs, but leaves and roots responded with different dynamics to rewatering. After a transient normalization of most metabolites within 8 days, a second accumulation of amino acids in leaves might indicate a stress imprint beneficial in upcoming drought events. Repair mechanisms seemed important during initial recovery and occurred at the expense of growth for at least 12 days. These results indicate that organ specific metabolic recovery responses might be related to distinct functions and concomitant disparate stress levels in above- and belowground organs. With respect to metabolism, recovery was not simply a reversal of the stress responses. PMID:29738573

75 FR 60715 - Domestic Sugar Program-FY 2010 and FY 2011 Cane Sugar and Beet Sugar Marketing Allotments and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-01

...,716 Hawaii Gay & Robinson, Inc 72,401 -18,673 -50,592 3,136 Hawaiian Commercial & Sugar Company... & Sons 495,489 Total Louisiana 1,620,472 Texas: Rio Grande Valley 182,094 Hawaii: Gay & Robinson, Inc 73...

Noteworthy Facts about a Methane-Producing Microbial Community Processing Acidic Effluent from Sugar Beet Molasses Fermentation.

PubMed

Chojnacka, Aleksandra; Szczęsny, Paweł; Błaszczyk, Mieczysław K; Zielenkiewicz, Urszula; Detman, Anna; Salamon, Agnieszka; Sikora, Anna

2015-01-01

Anaerobic digestion is a complex process involving hydrolysis, acidogenesis, acetogenesis and methanogenesis. The separation of the hydrogen-yielding (dark fermentation) and methane-yielding steps under controlled conditions permits the production of hydrogen and methane from biomass. The characterization of microbial communities developed in bioreactors is crucial for the understanding and optimization of fermentation processes. Previously we developed an effective system for hydrogen production based on long-term continuous microbial cultures grown on sugar beet molasses. Here, the acidic effluent from molasses fermentation was used as the substrate for methanogenesis in an upflow anaerobic sludge blanket bioreactor. This study focused on the molecular analysis of the methane-yielding community processing the non-gaseous products of molasses fermentation. The substrate for methanogenesis produces conditions that favor the hydrogenotrophic pathway of methane synthesis. Methane production results from syntrophic metabolism whose key process is hydrogen transfer between bacteria and methanogenic Archaea. High-throughput 454 pyrosequencing of total DNA isolated from the methanogenic microbial community and bioinformatic sequence analysis revealed that the domain Bacteria was dominated by Firmicutes (mainly Clostridia), Bacteroidetes, δ- and γ-Proteobacteria, Cloacimonetes and Spirochaetes. In the domain Archaea, the order Methanomicrobiales was predominant, with Methanoculleus as the most abundant genus. The second and third most abundant members of the Archaeal community were representatives of the Methanomassiliicoccales and the Methanosarcinales. Analysis of the methanogenic sludge by scanning electron microscopy with Energy Dispersive X-ray Spectroscopy and X-ray diffraction showed that it was composed of small highly heterogeneous mineral-rich granules. Mineral components of methanogenic granules probably modulate syntrophic metabolism and methanogenic

29 CFR 780.801 - Statutory provisions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar..., sugar-beet molasses, sugarcane, or maple sap, into sugar (other than refined sugar) or syrup. Section 13...

29 CFR 780.801 - Statutory provisions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar..., sugar-beet molasses, sugarcane, or maple sap, into sugar (other than refined sugar) or syrup. Section 13...

29 CFR 780.801 - Statutory provisions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar..., sugar-beet molasses, sugarcane, or maple sap, into sugar (other than refined sugar) or syrup. Section 13...

29 CFR 780.801 - Statutory provisions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar..., sugar-beet molasses, sugarcane, or maple sap, into sugar (other than refined sugar) or syrup. Section 13...

29 CFR 780.801 - Statutory provisions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar..., sugar-beet molasses, sugarcane, or maple sap, into sugar (other than refined sugar) or syrup. Section 13...

Can anaerobic digestion of sugar beet pulp support the circular economy? a study of biogas and nutrient potential

NASA Astrophysics Data System (ADS)

Suhartini, S.; Heaven, S.; Banks, C. J.

2018-03-01

Anaerobic digestion (AD), known as a biological process without oxygen to convert complex organic materials into biogas, is capable of processing large tonnage quantities of biomass, such as sugar beet pulp (SBP). In addition to biogas production, its use allows nutrients and organic carbon recycle back to agriculture through the spreading of digestate. Digestate still contains high amount of nutrients (N, P, K) for use as biofertiliser. The aims of this research were to determine biogas/methane potential as a baseline for comparison with performance in semi-continuous digestion, and to determine nutrient and potentially toxic elements (PTE) of digestate fractions with respect to their potential for utilisation in agriculture. The Biochemical Methane Potential (BMP) test was performed in triplicate against blank and positive controls over a period of 28 days with gas measured at regular intervals. Semi-continuous AD of SBP was operated under mesophilic and thermophilic condition for 206 and 165 days. The results indicated that SBP is a very promising feedstock for AD, with the average BMP of 0.321 l CH4 g-1 VS and biogas potential of 0.605 l g-1 VS. Under semi-continuous operation, SBP also demonstrated positive results. Digestates from mesophilic and thermophilic AD of SBP contained useful quantities of N, P and K, with an acceptable Ni concentration in accordance to limits for PTE. These results suggest that digestate has the potential to be utilised on agricultural and arable land. This study illustrated the positive effects of applying AD to the achievement of economic savings and environmental-friendly performance.

The Assessment of Red Beet as a Natural Colorant, and Evaluation of Quality Properties of Emulsified Pork Sausage Containing Red Beet Powder during Cold Storage

PubMed Central

Jin, Sang-Keun; Choi, Jung-Seok; Moon, Sung-Sil; Jeong, Jin-Yeon

2014-01-01

The purpose of this study was to assess red beet as a natural colorant in emulsified pork sausage and to investigate the effect of red beet on quality characteristics of emulsified pork sausage during 20 d of cold storage. Red beet was prepared as a powder and a substitute with sodium nitrite at 0.5% and 1.0% levels in emulsified pork sausage. Red beet significantly increased the moisture content and pH (p<0.0001) and affected color traits. Lightness of emulsified pork sausage decreased by the addition of red beet powder (p<0.01), whereas lightness with red beet treatments slightly increased during 20 d of cold storage at 4℃ (p<0.05). Redness dramatically increased with red beet powder (p<0.0001). Color by sensory evaluation also showed a significant effect from red beet addition (p<0.05), whereas the other sensory properties such as flavor, tenderness, juiciness, and overall acceptability were not affected by the addition of red beet powder (p>0.05). Texture and 2-thiobabituric acid reactive substance were also not affected by red beet addition (p>0.05). Therefore, red beet could be a good natural colorant in emulsified pork sausage but it needs additional processing, such as betalain concentration and extraction as a juice, to be used as an antioxidant in meat products. PMID:26761285

29 CFR 780.800 - Scope and significance of interpretative bulletin.

Code of Federal Regulations, 2011 CFR

2011-07-01

... STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane..., sugar-beet molasses, sugarcane or maple sap, into sugar (other than refined sugar) or syrup. The limited...

29 CFR 780.800 - Scope and significance of interpretative bulletin.

Code of Federal Regulations, 2010 CFR

2010-07-01

... STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane..., sugar-beet molasses, sugarcane or maple sap, into sugar (other than refined sugar) or syrup. The limited...

Photolabeling of Tonoplast from Sugar Beet Cell Suspensions by [3H]5-(N-Methyl-N-Isobutyl)-Amiloride, an Inhibitor of the Vacuolar Na+/H+ Antiport 1

PubMed Central

Barkla, Bronwyn J.; Charuk, Jeffrey H. M.; Cragoe, Edward J.; Blumwald, Eduardo

1990-01-01

The effects of 5-(N-methyl-N-isobutyl)-amiloride (MIA), an amiloride analog, was tested on the Na+/H+ antiport activity of intact vacuoles and tonoplast vesicles isolated from sugar beet (Beta vulgaris L.) cell suspension cultures. MIA inhibited Na+/H+ exchange in a competitive manner with a Ki of 2.5 and 5.9 micromolar for ΔpH-dependent 22Na+ influx in tonoplast vesicles and Na+-dependent H+ efflux in intact vacuoles, respectively. Scatchard analysis of the binding of [3H]MIA to tonoplast membranes revealed a high affinity binding component with a Kd of 1.3 micromolar. The close relationship between the dissociation constant value obtained and the constants of inhibition for MIA obtained by fluorescence quenching and isotope exchange suggests that the high affinity component represents a class of sites associated with the tonoplast Na+/H+ antiport. Photolabeling of the tonoplast with [3H]MIA revealed two sets of polypeptides with a different affinity to amiloride and its analog. Images Figure 7 PMID:16667602

Noteworthy Facts about a Methane-Producing Microbial Community Processing Acidic Effluent from Sugar Beet Molasses Fermentation

PubMed Central

Chojnacka, Aleksandra; Szczęsny, Paweł; Błaszczyk, Mieczysław K.; Zielenkiewicz, Urszula; Detman, Anna; Salamon, Agnieszka; Sikora, Anna

2015-01-01

Anaerobic digestion is a complex process involving hydrolysis, acidogenesis, acetogenesis and methanogenesis. The separation of the hydrogen-yielding (dark fermentation) and methane-yielding steps under controlled conditions permits the production of hydrogen and methane from biomass. The characterization of microbial communities developed in bioreactors is crucial for the understanding and optimization of fermentation processes. Previously we developed an effective system for hydrogen production based on long-term continuous microbial cultures grown on sugar beet molasses. Here, the acidic effluent from molasses fermentation was used as the substrate for methanogenesis in an upflow anaerobic sludge blanket bioreactor. This study focused on the molecular analysis of the methane-yielding community processing the non-gaseous products of molasses fermentation. The substrate for methanogenesis produces conditions that favor the hydrogenotrophic pathway of methane synthesis. Methane production results from syntrophic metabolism whose key process is hydrogen transfer between bacteria and methanogenic Archaea. High-throughput 454 pyrosequencing of total DNA isolated from the methanogenic microbial community and bioinformatic sequence analysis revealed that the domain Bacteria was dominated by Firmicutes (mainly Clostridia), Bacteroidetes, δ- and γ-Proteobacteria, Cloacimonetes and Spirochaetes. In the domain Archaea, the order Methanomicrobiales was predominant, with Methanoculleus as the most abundant genus. The second and third most abundant members of the Archaeal community were representatives of the Methanomassiliicoccales and the Methanosarcinales. Analysis of the methanogenic sludge by scanning electron microscopy with Energy Dispersive X-ray Spectroscopy and X-ray diffraction showed that it was composed of small highly heterogeneous mineral-rich granules. Mineral components of methanogenic granules probably modulate syntrophic metabolism and methanogenic

Isolation, one-step affinity purification, and characterization of a polyextremotolerant laccase from the halophilic bacterium Aquisalibacillus elongatus and its application in the delignification of sugar beet pulp.

PubMed

Rezaei, Shahla; Shahverdi, Ahmad Reza; Faramarzi, Mohammad Ali

2017-04-01

The aim of the present work was to study the ability of a halophilic bacterial laccase to efficient delignification in extreme conditions. Here, a highly stable extracellular laccase showing ligninolytic activity from halophilic Aquisalibacillus elongatus is described. The laccase production was strongly influenced by NaCl and CuSO 4 and under optimal conditions reached 4.8UmL -1 . The monomeric enzyme of 75kDa was purified by a synthetic affinity column with 68.2% yield and 99.8-fold purification. The enzyme showed some valuable features viz. stability against a wide range of organic solvents, salts, metals, inhibitors, and surfactants and specificity to a wide spectrum of substrates diverse in structure and redox potential. It retained more than 50% of the original activity at 25-75°C and pH 5.0-10.0. Furthermore, the enzyme was found to be effective in the delignification of sugar beet pulp in an ionic liquid that makes it useful for industrial applications. Copyright © 2017 Elsevier Ltd. All rights reserved.

Changes in the intraisolate genetic structure of Beet necrotic yellow vein virus populations associated with plant resistance breakdown.

PubMed

Acosta-Leal, Rodolfo; Fawley, Marvin W; Rush, Charles M

2008-06-20

The causal agent of rhizomania disease, Beet necrotic yellow vein virus (BNYVV), typically produces asymptomatic root-limited infections in sugar beets (Beta vulgaris) carrying the Rz1-allele. Unfortunately, this dominant resistance has been recently overcome. Multiple cDNA clones of the viral pathogenic determinant p25, derived from populations infecting susceptible or resistant plants, were sequenced to identify host effects on the viral population structure. Populations isolated from compatible plant-virus interactions (susceptible plant-wild type virus and resistant plant-resistant breaking variants) were large and relatively homogeneous, whereas those from the incompatible interaction (resistant plant-avirulent type virus) were small and highly heterogeneous. All populations from susceptible plants had the same dominant haplotype, whereas those from resistant cultivars had a different haplotype surrounded by a spectrum of mutants. Selection and diversification analyses suggest an evolutionary trajectory of BNYVV with positive selection for changes required to overcome resistance, followed by elimination of hitchhiking mutations through purifying selection.

40 CFR 409.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing... a point source where the sugar beet processing capacity of the point source does not exceed 1090 kkg... results, in whole or in part, from barometric condensing operations and any other beet sugar processing...

40 CFR 409.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing... a point source where the sugar beet processing capacity of the point source does not exceed 1090 kkg... results, in whole or in part, from barometric condensing operations and any other beet sugar processing...

40 CFR 409.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing... a point source where the sugar beet processing capacity of the point source does not exceed 1090 kkg... results, in whole or in part, from barometric condensing operations and any other beet sugar processing...

40 CFR 409.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing... a point source where the sugar beet processing capacity of the point source does not exceed 1090 kkg... results, in whole or in part, from barometric condensing operations and any other beet sugar processing...

40 CFR 409.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar Processing... a point source where the sugar beet processing capacity of the point source does not exceed 1090 kkg... results, in whole or in part, from barometric condensing operations and any other beet sugar processing...

40 CFR 180.411 - Fluazifop-P-butyl; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Beet, sugar, dried pulp 1.0 Beet, sugar, molasses 3.5 Beet, sugar, roots 0.25 Carrot, roots 2.0 Cattle....05 Soybean, seed 2.5 Sweet potato, roots 0.05 1 No U.S. registrations. (b) Section 18 emergency...

40 CFR 180.411 - Fluazifop-P-butyl; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Beet, sugar, dried pulp 1.0 Beet, sugar, molasses 3.5 Beet, sugar, roots 0.25 Carrot, roots 2.0 Cattle....05 Soybean, seed 2.5 Sweet potato, roots 0.05 1 No U.S. registrations. (b) Section 18 emergency...

The increase of the fertility of soils using the liquid organic fertilizers and fertilizers based on sugar-beet wastes.

NASA Astrophysics Data System (ADS)

Vyborova, Oxana

2010-05-01

physical, physicochemical properties of soils, its air, water and thermal rate. Humic acids with mineral and organomineral particles of soil form the soil absorbent complex. The inclusion of humic fertilizers promotes the process when humic substances form a very valuable water-stable clumpy-granular structure, which improves water-carrying and water-holding capacity, its air permeability by agglutination of mineral particles with each other. The soils, where humic fertilizers are carried in soils regularly, are more stable for influence of chemical polluting substances (for example, radioactive nuclides, heavy metals, pesticides) than poor soils. The inclusion of humic fertilizers is very important in period of urbanization and cropping on the plough-lands not far from a big industrial area. The lignitic materials tie together the detrimental compounds formed the insoluble complex in soil solution. The detrimental compounds don't go into plants, subsoil waters and atmosphere. The lignitic watering of soils (in concentration from 0.1 to 0.01%) increases biological activity of soil in a man-caused zones and it promotes to stability of plants to detrimental emission of enterprises. Today the problem of processing of sugar-beet industry is very important. In the result of storing sugar-beet wastes the pollution of environment is occurred, examples of this pollution are gassing, salinization of soils and ground waters by filtrational sediments. One of these wastes is defecation sludge. The defecation sludge consists of CaCO3, organic matter, nitrogen, phosphorus, potassium and microelements. The technology of receiving N-Ca fertilizer based on defecate was developed because of impossibility of using this waste in pure form. For available data, using of these fertilizers improves the soil fertility and degree of pollution by heavy metals don't exceed an acceptance limits.

40 CFR 180.353 - Desmedipham; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... agricultural commodities in the table that follows: Commodity Parts per million Beet, garden, roots 0.05 Beet, garden, tops 1.0 Beet, sugar, roots 0.1 Beet, sugar, tops 5.0 Spinach 6.0 (b) Section 18 emergency...

40 CFR 180.353 - Desmedipham; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... agricultural commodities in the table that follows: Commodity Parts per million Beet, garden, roots 0.05 Beet, garden, tops 1.0 Beet, sugar, roots 0.1 Beet, sugar, tops 5.0 Spinach 6.0 (b) Section 18 emergency...

40 CFR 180.353 - Desmedipham; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

... agricultural commodities in the table that follows: Commodity Parts per million Beet, garden, roots 0.05 Beet, garden, tops 1.0 Beet, sugar, roots 0.1 Beet, sugar, tops 5.0 Spinach 6.0 (b) Section 18 emergency...

40 CFR 180.353 - Desmedipham; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... agricultural commodities in the table that follows: Commodity Parts per million Beet, garden, roots 0.05 Beet, garden, tops 1.0 Beet, sugar, roots 0.1 Beet, sugar, tops 5.0 Spinach 6.0 (b) Section 18 emergency...

40 CFR 180.353 - Desmedipham; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... agricultural commodities in the table that follows: Commodity Parts per million Beet, garden, roots 0.05 Beet, garden, tops 1.0 Beet, sugar, roots 0.1 Beet, sugar, tops 5.0 Spinach 6.0 (b) Section 18 emergency...

Immunological Evidence for the Existence of a Carrier Protein for Sucrose Transport in Tonoplast Vesicles from Red Beet (Beta vulgaris L.) Root Storage Tissue.

PubMed Central

Getz, H. P.; Grosclaude, J.; Kurkdjian, A.; Lelievre, F.; Maretzki, A.; Guern, J.

1993-01-01

Monoclonal antibodies were raised in mice against a highly purified tonoplast fraction from isolated red beet (Beta vulgaris L. ssp. conditiva) root vacuoles. Positive hybridoma clones and sub-clones were identified by prescreening using an enzyme-linked immunosorbent assay (ELISA) and by postscreening using a functional assay. This functional assay consisted of testing the impact of hybridoma supernatants and antibody-containing ascites fluids on basal and ATP-stimulated sugar uptake in vacuoles, isolated from protoplasts, as well as in tonoplast vesicles, prepared from tissue homogenates of red beet roots. Antibodies from four clones were particularly positive in ELISAs and they inhibited sucrose uptake significantly. These antibodies were specific inhibitors of sucrose transport, but they exhibited relatively low membrane and species specificity since uptake into red beet root protoplasts and sugarcane tonoplast vesicles was inhibited as well. Fast protein liquid chromatography assisted size exclusion chromatography on Superose 6 columns yielded two major peaks in the 55 to 65-kD regions and in the 110- to 130-kD regions of solubilized proteins from red beet root tonoplasts, which reacted positively in immunoglobulin-M(IgM)-specific ELISAs with anti-sugarcane tonoplast monoclonal IgM antibodies. Only reconstituted proteoliposomes containing polypeptides from the 55- to 65-kD band took up [14C]-sucrose with linear rates for 2 min, suggesting that this fraction contains the tonoplast sucrose carrier. PMID:12231863

Milled industrial beet color kinetics and total soluble solid contents by image analysis

USDA-ARS?s Scientific Manuscript database

Industrial beets are an emerging feedstock for biofuel and bioproducts industry in the US. Milling of industrial beets is the primary step in front end processing (FEP) for ethanol production. Milled beets undergo multiple pressings with water addition during raw beet juice extraction, and extracted...

40 CFR 180.242 - Thiabendazole; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., dry, seed 0.1 None Beet, sugar, dried pulp 3.5 12/25/10 Beet, sugar, roots 0.25 12/25/10 Beet, sugar... Strawberry1 5.0 None Sweet potato (postharvest to sweet potato intended only for use as seed) 0.05 None Wheat...

Beet yellow stunt

USDA-ARS?s Scientific Manuscript database

Beet yellow stunt virus (BYSV) is a potentially destructive yellows-type virus affecting plants in the family Asteraceae. The virus is a member of the genus Closterovirus, family Closteroviridae, and has been found in California and England. Initial symptoms consist of chlorosis of the older leaves,...

Analysis of drought-tolerant sugar beet (Beta vulgaris L.) mutants induced with gamma radiation using SDS-PAGE and ISSR markers.

PubMed

Sen, Ayse; Alikamanoglu, Sema

2012-01-01

Drought is one of the major environmental stresses which greatly affect the plant growth and productivity. In the present study, various doses (0-75Gy) of gamma rays were applied to investigate the effect of radiation on shoot tip explants. It was observed that the regeneration rates and plant fresh weights decreased significantly with an increase in radiation dose. The optimal irradiation doses for mutation induction were determined at 15 and 20Gy. Afterwards, the induction of somatic mutation in sugar beet (Beta vulgaris L.) was investigated by irradiation of shoot tips with 15 and 20Gy gamma rays. Irradiated shoot tips were sub-cultured and M(1)V(1)-M(1)V(3) generations were obtained. Mutants tolerant to drought stress were selected on MS medium, supplemented with 10 and 20gl(-1) PEG6000. Of the M(1)V(3) plantlets, drought-tolerant mutants were selected. Leaf soluble proteins obtained from the control and drought-tolerant mutants were analyzed by SDS-PAGE. A total of 22 protein bands were determined and 2 of them were observed to be drought-tolerant mutants except the control. Polymorphism was also detected among the control and drought-tolerant mutants by DNA fingerprinting using ISSR markers. A total of 106 PCR fragments were amplified with 19 ISSR primers and 91 of them were polymorphic. The dendrograms were separated into two main clusters. First cluster included M8 mutant plant, which was applied 20Gy gamma radiation and regenerated on selective culture media containing 10gl(-1) PEG6000 concentration, and the second cluster was further divided into five sub-clusters. Copyright © 2012 Elsevier B.V. All rights reserved.

40 CFR 180.464 - Dimethenamid; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., sugar, dried pulp 0.01 Beet, sugar, molasses 0.01 Beet, sugar, roots 0.01 Beet, sugar, tops 0.01 Corn, field, forage 0.01 Corn, field, grain 0.01 Corn, field, stover 0.01 Corn, pop, forage 0.01 Corn, pop, grain 0.01 Corn, pop, stover 0.01 Corn, sweet, forage 0.01 Corn, sweet, kernel plus cob with husks...

40 CFR 180.470 - Acetochlor; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., sugar, dried pulp 0.50 Beet, sugar, molasses 0.80 Beet, sugar, roots 0.30 Beet, sugar, tops 0.70 Corn, field, forage 4.5 Corn, field, grain 0.05 Corn, field, stover 2.5 Corn, pop, grain 0.05 Corn, pop, stover 2.5 Corn, sweet, forage 1.5 Corn, sweet, kernels plus cob with husks removed 0.05 Corn, sweet...

40 CFR 180.464 - Dimethenamid; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., sugar, dried pulp 0.01 Beet, sugar, molasses 0.01 Beet, sugar, roots 0.01 Beet, sugar, tops 0.01 Corn, field, forage 0.01 Corn, field, grain 0.01 Corn, field, stover 0.01 Corn, pop, forage 0.01 Corn, pop, grain 0.01 Corn, pop, stover 0.01 Corn, sweet, forage 0.01 Corn, sweet, kernel plus cob with husks...

40 CFR 180.464 - Dimethenamid; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., sugar, dried pulp 0.01 Beet, sugar, molasses 0.01 Beet, sugar, roots 0.01 Beet, sugar, tops 0.01 Corn, field, forage 0.01 Corn, field, grain 0.01 Corn, field, stover 0.01 Corn, pop, forage 0.01 Corn, pop, grain 0.01 Corn, pop, stover 0.01 Corn, sweet, forage 0.01 Corn, sweet, kernel plus cob with husks...

76 FR 6759 - Monsanto Company and KWS SAAT AG; Decision With Respect to the Petition for Partial Deregulation...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-08

... Genetically Engineered Roundup Ready Sugar Beets AGENCY: Animal and Plant Health Inspection Service, USDA... Ready[supreg] sugar beets developed by the Monsanto Company (Monsanto) and KWS SAAT AG (KWS), designated.... APHIS will grant a partial deregulation for event H7-1 sugar beet root crop production activities when...

40 CFR 409.12 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar... beet sugar processing operation. Effluent characteristic Effluent limitations Maximum for any 1 day...

40 CFR 409.12 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar... beet sugar processing operation. Effluent characteristic Effluent limitations Maximum for any 1 day...

40 CFR 409.12 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar... beet sugar processing operation. Effluent characteristic Effluent limitations Maximum for any 1 day...

40 CFR 409.12 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar... beet sugar processing operation. Effluent characteristic Effluent limitations Maximum for any 1 day...

40 CFR 409.12 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SUGAR PROCESSING POINT SOURCE CATEGORY Beet Sugar... beet sugar processing operation. Effluent characteristic Effluent limitations Maximum for any 1 day...

40 CFR 180.472 - Imidacloprid; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances... 1.0 Banana 0.50 Beet, sugar, molasses 0.30 Beet, sugar, roots 0.05 Beet, sugar, tops 0.50 Biriba 0... Persimmon 3.0 Pistachio 0.05 Pomegranate 0.90 Potato, chip 0.40 Potato, processed potato waste 0.90 Poultry...

40 CFR 180.472 - Imidacloprid; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances... 1.0 Banana 0.50 Beet, sugar, molasses 0.30 Beet, sugar, roots 0.05 Beet, sugar, tops 0.50 Biriba 0....75 Pecan 0.05 Persimmon 3.0 Pistachio 0.05 Pomegranate 0.90 Potato, chip 0.40 Potato, processed...

40 CFR 180.472 - Imidacloprid; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances... 1.0 Banana 0.50 Beet, sugar, molasses 0.30 Beet, sugar, roots 0.05 Beet, sugar, tops 0.50 Biriba 0....75 Pecan 0.05 Persimmon 3.0 Pistachio 0.05 Pomegranate 0.90 Potato, chip 0.40 Potato, processed...

Improved detection of sugar addition to maple syrup using malic acid as internal standard and in 13C isotope ratio mass spectrometry (IRMS).

PubMed

Tremblay, Patrice; Paquin, Réal

2007-01-24

Stable carbon isotope ratio mass spectrometry (delta13C IRMS) was used to detect maple syrup adulteration by exogenous sugar addition (beet and cane sugar). Malic acid present in maple syrup is proposed as an isotopic internal standard to improve actual adulteration detection levels. A lead precipitation method has been modified to isolate quantitatively malic acid from maple syrup using preparative reversed-phase liquid chromatography. The stable carbon isotopic ratio of malic acid isolated from this procedure shows an excellent accuracy and repeatability of 0.01 and 0.1 per thousand respectively, confirming that the modified lead precipitation method is an isotopic fractionation-free process. A new approach is proposed to detect adulteration based on the correlation existing between the delta13Cmalic acid and the delta13Csugars-delta13Cmalic acid (r = 0.704). This technique has been tested on a set of 56 authentic maple syrup samples. Additionally, authentic samples were spiked with exogeneous sugars. The mean theoretical detection level was statistically lowered using this technique in comparison with the usual two-standard deviation approach, especially when maple syrup is adulterated with beet sugar : 24 +/- 12% of adulteration detection versus 48 +/- 20% (t-test, p = 7.3 x 10-15). The method was also applied to published data for pineapple juices and honey with the same improvement.

7 CFR 1435.104 - Loan maintenance.

Code of Federal Regulations, 2013 CFR

2013-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.104... result of the execution of security agreements by sugarcane and sugar beet processors shall be superior to all statutory and common law liens on raw cane sugar, refined beet sugar, and in-process sugar for...

7 CFR 1435.104 - Loan maintenance.

Code of Federal Regulations, 2014 CFR

2014-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.104... result of the execution of security agreements by sugarcane and sugar beet processors shall be superior to all statutory and common law liens on raw cane sugar, refined beet sugar, and in-process sugar for...

7 CFR 1435.104 - Loan maintenance.

Code of Federal Regulations, 2012 CFR

2012-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.104... result of the execution of security agreements by sugarcane and sugar beet processors shall be superior to all statutory and common law liens on raw cane sugar, refined beet sugar, and in-process sugar for...

7 CFR 1435.104 - Loan maintenance.

Code of Federal Regulations, 2011 CFR

2011-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.104... result of the execution of security agreements by sugarcane and sugar beet processors shall be superior to all statutory and common law liens on raw cane sugar, refined beet sugar, and in-process sugar for...

7 CFR 1435.104 - Loan maintenance.

Code of Federal Regulations, 2010 CFR

2010-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.104... result of the execution of security agreements by sugarcane and sugar beet processors shall be superior to all statutory and common law liens on raw cane sugar, refined beet sugar, and in-process sugar for...

7 CFR 1435.309 - Reassignment of deficits.

Code of Federal Regulations, 2014 CFR

2014-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.309 Reassignment of deficits. (a) CCC will determine, from time to time, whether sugar beet or sugarcane processors will be unable to market their allocations. (b) Sugar beet and sugar cane...

7 CFR 1435.500 - General statement.

Code of Federal Regulations, 2013 CFR

2013-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Processor Sugar Payment-In-Kind (PIK) Program § 1435.500 General statement. This subpart shall be applicable to sugar beet and... sugarcane or sugar beets processed by the processors, reduce sugar production in return for a payment of...

7 CFR 1435.309 - Reassignment of deficits.

Code of Federal Regulations, 2012 CFR

2012-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.309 Reassignment of deficits. (a) CCC will determine, from time to time, whether sugar beet or sugarcane processors will be unable to market their allocations. (b) Sugar beet and sugar cane...

7 CFR 1435.309 - Reassignment of deficits.

Code of Federal Regulations, 2013 CFR

2013-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.309 Reassignment of deficits. (a) CCC will determine, from time to time, whether sugar beet or sugarcane processors will be unable to market their allocations. (b) Sugar beet and sugar cane...

7 CFR 1435.309 - Reassignment of deficits.

Code of Federal Regulations, 2011 CFR

2011-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.309 Reassignment of deficits. (a) CCC will determine, from time to time, whether sugar beet or sugarcane processors will be unable to market their allocations. (b) Sugar beet and sugar cane...

7 CFR 1435.309 - Reassignment of deficits.

Code of Federal Regulations, 2010 CFR

2010-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.309 Reassignment of deficits. (a) CCC will determine, from time to time, whether sugar beet or sugarcane processors will be unable to market their allocations. (b) Sugar beet and sugar cane...

7 CFR 1435.500 - General statement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Processor Sugar Payment-In-Kind (PIK) Program § 1435.500 General statement. This subpart shall be applicable to sugar beet and... sugarcane or sugar beets processed by the processors, reduce sugar production in return for a payment of...

Structural and Physiological Changes in Sugar Beet Leaves during Sink to Source Conversion 1

PubMed Central

Fellows, Robert J.; Geiger, Donald R.

1974-01-01

The onset of export during leaf development was correlated with changes in metabolism and ultrastructure and with patterns of solute distribution in the developing seventh leaf of sugar beet (Beta vulgaris L.) in order to study the cause of initiation of translocation. Infrared gas analysis of carbon dioxide uptake showed a broad peak for net photosynthesis dm−2 at 35 to 40% final laminar length. Pulse labeling with 14CO2 demonstrated that maximum import of translocate occurred at 25% final laminar length; export was first observed at 35% final laminar length. Between 40 and 50% final laminar length a rapid increase in amount of export occurred, primarily as a result of the increase in the area of leaf which was exporting. Whole leaf autoradiography revealed that onset of phloem loading spread basipetally from the leaf tip; loading was initiated at about 22% final laminar length and was essentially complete by 50% final laminar length. Those areas which clearly exhibited loading no longer imported from other parts of the plant while the area in transition still appeared to import label from source regions. There was little difference between source and sink leaf tissue in the kinetic parameters Kj and Jmax (30) for uptake of exogenous sucrose supplied via free space. The concentration of solutes in sieve elements and companion cells of the sink leaf was highest in the mature tip area and gradually decreased in the direction of the immature base. There appeared to be no dramatic structural transformation within the phloem of the minor veins that was closely correlated with the time when phloem loading or export began. Rather, there appeared to be a gradual differentiation of phloem which resulted in a sizable proportion of the population of minor vein sieve elements and companion cells attaining maturity in the older sink regions prior to initiation of phloem loading. The area of the leaf undergoing development appeared to exhibit the beginnings of phloem loading

Metabolic effects of dietary sugar beet pulp or wheat bran in growing female pigs.

PubMed

Weber, T E; Kerr, B J

2012-02-01

An experiment was conducted to determine the effects of feeding a moderate level of 2 different fiber sources on energy metabolites; mitochondrial biogenesis in the intestine, liver, and muscle; and the expression of some genes that regulate energy metabolism in intestine, liver, muscle, and adipose tissue. Female pigs (n = 36; BW = 15.0 ± 0.7 kg) were fed diets containing no added fiber, 12.5% sugar beet pulp (SBP), or 12.5% wheat bran (WB) for 24 d. Blood samples were collected on d 7 and 24 for cholesterol, glucose, NEFA, and triglyceride analyses. At completion of the experiment, ileum, colon, subcutaneous adipose, and LM samples were obtained from a subset (n = 6) of pigs fed each diet for analysis of tissue mitochondrial DNA (mtDNA) content and mRNA abundance by quantitative real-time reverse-transcription PCR. Glycogen and triglyceride content of liver and LM were determined, and colon content VFA was also determined. The addition of SBP or WB to the diet had no effect (P > 0.55) on ADG, ADFI, or G:F. Serum NEFA and triglycerides were increased (P < 0.05) in pigs fed SBP compared with pigs fed the control diet or WB on d 7, and NEFA remained increased (P < 0.05) on d 24 in pigs fed SBP. Dietary fiber had no effect (P > 0.24) on glycogen and triglyceride content of liver or LM, but colonic acetate concentrations were increased (P < 0.05) in pigs fed either SBP or WB. Pigs fed WB had an increased (P < 0.05) mtDNA content in ileum tissue and increased (P < 0.05) citrate synthase mRNA in colon tissue. In the liver, feeding either SBP or WB led to a decrease (P < 0.05) in mtDNA content, whereas feeding WB decreased (P < 0.05) mtDNA abundance in the LM, and feeding either SBP or WB decreased (P < 0.05) expression of citrate synthase mRNA. Quantitative reverse-transcription PCR revealed that feeding WB increased (P < 0.05) proliferating cell nuclear antigen mRNA abundance in the ileum and colon. Feeding WB increased (P < 0.05) mRNA abundance of a regulator of

Environmental assessment of mild bisulfite pretreatment of forest residues into fermentable sugars for biofuel production.

PubMed

Nwaneshiudu, Ikechukwu C; Ganguly, Indroneil; Pierobon, Francesca; Bowers, Tait; Eastin, Ivan

2016-01-01

Sugar production via pretreatment and enzymatic hydrolysis of cellulosic feedstock, in this case softwood harvest residues, is a critical step in the biochemical conversion pathway towards drop-in biofuels. Mild bisulfite (MBS) pretreatment is an emerging option for the breakdown and subsequent processing of biomass towards fermentable sugars. An environmental assessment of this process is critical to discern its future sustainability in the ever-changing biofuels landscape. The subsequent cradle-to-gate assessment of a proposed sugar production facility analyzes sugar made from woody biomass using MBS pretreatment across all seven impact categories (functional unit 1 kg dry mass sugar), with a specific focus on potential global warming and eutrophication impacts. The study found that the eutrophication impact (0.000201 kg N equivalent) is less than the impacts from conventional beet and cane sugars, while the global warming impact (0.353 kg CO2 equivalent) falls within the range of conventional processes. This work discusses some of the environmental impacts of designing and operating a sugar production facility that uses MBS as a method of treating cellulosic forest residuals. The impacts of each unit process in the proposed facility are highlighted. A comparison to other sugar-making process is detailed and will inform the growing biofuels literature.

Effect of curtovirus species competitiveness in host plants on transmission and incidence of Beet severe curly top virus and Beet mild curly top virus

USDA-ARS?s Scientific Manuscript database

Curly top disease, caused by viruses in the genus Curtovirus, causes significant economic losses for sugarbeet and other crops throughout the western United States. Recent studies demonstrated the two most abundant curtovirus species in the US are Beet severe curly top virus (BSCTV) and Beet mild c...

Microbiology of sugar-rich environments: diversity, ecology and system constraints.

PubMed

Lievens, Bart; Hallsworth, John E; Pozo, Maria I; Belgacem, Zouhaier Ben; Stevenson, Andrew; Willems, Kris A; Jacquemyn, Hans

2015-02-01

Microbial habitats that contain an excess of carbohydrate in the form of sugar are widespread in the microbial biosphere. Depending on the type of sugar, prevailing water activity and other substances present, sugar-rich environments can be highly dynamic or relatively stable, osmotically stressful, and/or destabilizing for macromolecular systems, and can thereby strongly impact the microbial ecology. Here, we review the microbiology of different high-sugar habitats, including their microbial diversity and physicochemical parameters, which act to impact microbial community assembly and constrain the ecosystem. Saturated sugar beet juice and floral nectar are used as case studies to explore the differences between the microbial ecologies of low and higher water-activity habitats respectively. Nectar is a paradigm of an open, dynamic and biodiverse habitat populated by many microbial taxa, often yeasts and bacteria such as, amongst many others, Metschnikowia spp. and Acinetobacter spp., respectively. By contrast, thick juice is a relatively stable, species-poor habitat and is typically dominated by a single, xerotolerant bacterium (Tetragenococcus halophilus). A number of high-sugar habitats contain chaotropic solutes (e.g. ethyl acetate, phenols, ethanol, fructose and glycerol) and hydrophobic stressors (e.g. ethyl octanoate, hexane, octanol and isoamyl acetate), all of which can induce chaotropicity-mediated stresses that inhibit or prevent multiplication of microbes. Additionally, temperature, pH, nutrition, microbial dispersion and habitat history can determine or constrain the microbiology of high-sugar milieux. Findings are discussed in relation to a number of unanswered scientific questions. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

29 CFR 780.817 - Employees engaged in processing.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... of raw sugar and molasses: Juice weighing and measurement, heating, clarification, filtration, evaporating, crystallization, centrifuging, and handling and storing the raw sugar or molasses at the plant...

29 CFR 780.817 - Employees engaged in processing.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... of raw sugar and molasses: Juice weighing and measurement, heating, clarification, filtration, evaporating, crystallization, centrifuging, and handling and storing the raw sugar or molasses at the plant...

29 CFR 780.818 - Employees not engaged in processing.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... called the “boarding house”). (c) Hauling raw sugar or molasses away from the mill. (d) Any work outside...

29 CFR 780.818 - Employees not engaged in processing.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap... called the “boarding house”). (c) Hauling raw sugar or molasses away from the mill. (d) Any work outside...

40 CFR 180.364 - Glyphosate; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., shoots 0.2 Banana 0.2 Barley, bran 30 Beet, sugar, dried pulp 25 Beet, sugar, roots 10 Beet, sugar, tops..., roots 0.2 Ginger, white, flower 0.2 Gourd, buffalo, seed 0.1 Governor's plum 0.2 Gow kee, leaves 0.2... Mamey apple 0.2 Mango 0.2 Mangosteen 0.2 Marmaladebox 0.2 Meadowfoam, seed 0.1 Mioga, flower 0.2 Mustard...

40 CFR 180.242 - Thiabendazole; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., dry, seed 0.1 None Beet, sugar, dried pulp 3.5 12/25/10 Beet, sugar, roots 0.25 12/25/10 Beet, sugar..., forage 0.01 None Corn, pop, grain 0.01 None Corn, pop, stover 0.01 None Corn, sweet, forage 0.01 None Corn, sweet, kernels plus cop with husks removed 0.01 None Corn, sweet, stover 0.01 None Fruit, citrus...

40 CFR 180.242 - Thiabendazole; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., dry, seed 0.1 None Beet, sugar, dried pulp 3.5 12/25/10 Beet, sugar, roots 0.25 12/25/10 Beet, sugar..., forage 0.01 None Corn, pop, grain 0.01 None Corn, pop, stover 0.01 None Corn, sweet, forage 0.01 None Corn, sweet, kernels plus cop with husks removed 0.01 None Corn, sweet, stover 0.01 None Fruit, citrus...

40 CFR 180.242 - Thiabendazole; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., dry, seed 0.1 None Beet, sugar, dried pulp 3.5 12/25/10 Beet, sugar, roots 0.25 12/25/10 Beet, sugar..., forage 0.01 None Corn, pop, grain 0.01 None Corn, pop, stover 0.01 None Corn, sweet, forage 0.01 None Corn, sweet, kernels plus cop with husks removed 0.01 None Corn, sweet, stover 0.01 None Fruit, citrus...

40 CFR 180.242 - Thiabendazole; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., dry, seed 0.1 None Beet, sugar, dried pulp 3.5 12/25/10 Beet, sugar, roots 0.25 12/25/10 Beet, sugar..., forage 0.01 None Corn, pop, grain 0.01 None Corn, pop, stover 0.01 None Corn, sweet, forage 0.01 None Corn, sweet, kernels plus cop with husks removed 0.01 None Corn, sweet, stover 0.01 None Fruit, citrus...

Reducing added sugar intake increases the relative reinforcing value of high-sugar foods

USDA-ARS?s Scientific Manuscript database

Objective: To determine whether reducing added sugar intake to <10% of calories for 1 week changes the relative reinforcing value (RRV) of foods high in sugar and to test whether changes in RRV of high-sugar foods differed between non-overweight and obese adults. Background: The 2015-2020 DGA focu...

[Betaine-enriched beet suppresses hyperhomocysteinemia induced by choline deficiency in rats].

PubMed

Liu, Yiqun; Han, Feng; Sun, Licui; Lu, Jiaxi; Wang, Qin; Sugiyama, Kimio; Huang, Zhenwu

2015-03-01

To investigate the dose-dependent effects of beet powder supplementation on hyperhomocysteinemia induced by choline deprivation in rats. Methods 48 rats of the Wistar were fed 25% soybean protein diet (25S), choline deprivation in 25S diets (25SCD) with different betaine levels (0. 05% and 0. 1%) and beet powder levels (4. 12% and 8. 24%) corresponds to betaine levels for 10 days, and they were killed by decapitation to obtain blood and livers was subject to analysis the concentration of homocysteine, cysteine and other amino acids, as well as BHMT and CBS activities. The homocysteine concentration was increased from (11. 8 ± 0. 4) µmol/L to (33. 2 ± 0. 6) µmol/L by choline deprived - 25S diets (P < 0. 05). The choline deprivation-induced enhancement of plasma homocysteine concentration in rats fed 25S diet was significantly suppressed by 0. 10% betaine or 8. 24% beet in a dose dependent manner. Supplementation with betaine or beet significant increased hepatic BHMT activity. The results indicated that betaine or beet could completely suppress the hyperhomocysteinemia induced by choline deficiency resulting from stimulating the homocysteine removal by both remethylation and cystathionine formation.

Ozonation as an effective way to stabilize new kinds of fermentation media used in biotechnological production of liquid fuel additives.

PubMed

Dziugan, Piotr; Balcerek, Maria; Binczarski, Michal J; Kregiel, Dorota; Kucner, Marcin; Kunicka-Styczynska, Alina; Pielech-Przybylska, Katarzyna; Smigielski, Krzysztof; Witonska, Izabela A

2016-01-01

Intermediates from processing sugar beets are considered an attractive feedstock for ethanol fermentation due to their high fermentable sugar content. In particular, medium prepared from raw sugar beet juice seems to be suitable for use in fermentation processes, but it is microbiologically unstable and requires sterilization. This study investigates the effect of ozone treatment on the activity of microbial cells from Bacillus subtilis, Leuconostoc mesenteroides, Geobacillus stearothermophilus, Candida vini, and Aspergillus brasiliensis in raw sugar beet juice. Raw sugar beet juice contaminated with 10(5) cfu/mL of the microbial strains was treated with gaseous ozone (ozone concentration in the oxygen stream 0.1 g O3/L O2, flow rate 6 L/h, 10-30 min, 18-20 °C). The number of microflora decreased to 0 cfu/mL after 30 min of ozone treatment in all studied samples. Medium prepared from raw sugar beet juice and sterilized by ozonation is suitable for use in fermentation processes.

7 CFR 1435.102 - Eligibility requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.102... sugar beets or sugarcane, including share rent landowners, at both the time of harvest and the time of... CFR part 718. (b) In addition to all other provisions of this part, a sugar beet or sugarcane...

7 CFR 1435.102 - Eligibility requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.102... sugar beets or sugarcane, including share rent landowners, at both the time of harvest and the time of... CFR part 718. (b) In addition to all other provisions of this part, a sugar beet or sugarcane...

7 CFR 1435.102 - Eligibility requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.102... sugar beets or sugarcane, including share rent landowners, at both the time of harvest and the time of... CFR part 718. (b) In addition to all other provisions of this part, a sugar beet or sugarcane...

7 CFR 1435.102 - Eligibility requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.102... sugar beets or sugarcane, including share rent landowners, at both the time of harvest and the time of... CFR part 718. (b) In addition to all other provisions of this part, a sugar beet or sugarcane...

7 CFR 1435.102 - Eligibility requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Sugar Loan Program § 1435.102... sugar beets or sugarcane, including share rent landowners, at both the time of harvest and the time of... CFR part 718. (b) In addition to all other provisions of this part, a sugar beet or sugarcane...

Wavelet analysis techniques applied to removing varying spectroscopic background in calibration model for pear sugar content

NASA Astrophysics Data System (ADS)

Liu, Yande; Ying, Yibin; Lu, Huishan; Fu, Xiaping

2005-11-01

A new method is proposed to eliminate the varying background and noise simultaneously for multivariate calibration of Fourier transform near infrared (FT-NIR) spectral signals. An ideal spectrum signal prototype was constructed based on the FT-NIR spectrum of fruit sugar content measurement. The performances of wavelet based threshold de-noising approaches via different combinations of wavelet base functions were compared. Three families of wavelet base function (Daubechies, Symlets and Coiflets) were applied to estimate the performance of those wavelet bases and threshold selection rules by a series of experiments. The experimental results show that the best de-noising performance is reached via the combinations of Daubechies 4 or Symlet 4 wavelet base function. Based on the optimization parameter, wavelet regression models for sugar content of pear were also developed and result in a smaller prediction error than a traditional Partial Least Squares Regression (PLSR) mode.

The water footprint of sweeteners and bio-ethanol.

PubMed

Gerbens-Leenes, Winnie; Hoekstra, Arjen Y

2012-04-01

An increasing demand for food together with a growing demand for energy crops result in an increasing demand for and competition over water. Sugar cane, sugar beet and maize are not only essential food crops, but also important feedstock for bio-ethanol. Crop growth requires water, a scarce resource. This study aims to assess the green, blue and grey water footprint (WF) of sweeteners and bio-ethanol from sugar cane, sugar beet and maize in the main producing countries. The WFs of sweeteners and bio-ethanol are mainly determined by the crop type that is used as a source and by agricultural practise and agro-climatic conditions; process water footprints are relatively small. The weighted global average WF of sugar cane is 209 m(3)/tonne; for sugar beet this is 133 m(3)/tonne and for maize 1222 m(3)/tonne. Large regional differences in WFs indicate that WFs of crops for sweeteners and bio-ethanol can be improved. It is more favourable to use maize as a feedstock for sweeteners or bio-ethanol than sugar beet or sugar cane. The WF of sugar cane contributes to water stress in the Indus and Ganges basins. In the Ukraine, the large grey WF of sugar beet contributes to water pollution. In some western European countries, blue WFs of sugar beet and maize need a large amount of available blue water for agriculture. The allocation of the limited global water resources to bio-energy on a large scale will be at the cost of water allocation to food and nature. Copyright © 2011 Elsevier Ltd. All rights reserved.

Agroinoculation of Beet necrotic yellow vein virus cDNA clones results in plant systemic infection and efficient Polymyxa betae transmission.

PubMed

Delbianco, Alice; Lanzoni, Chiara; Klein, Elodie; Rubies Autonell, Concepcion; Gilmer, David; Ratti, Claudio

2013-05-01

Agroinoculation is a quick and easy method for the infection of plants with viruses. This method involves the infiltration of tissue with a suspension of Agrobacterium tumefaciens carrying binary plasmids harbouring full-length cDNA copies of viral genome components. When transferred into host cells, transcription of the cDNA produces RNA copies of the viral genome that initiate infection. We produced full-length cDNA corresponding to Beet necrotic yellow vein virus (BNYVV) RNAs and derived replicon vectors expressing viral and fluorescent proteins in pJL89 binary plasmid under the control of the Cauliflower mosaic virus 35S promoter. We infected Nicotiana benthamiana and Beta macrocarpa plants with BNYVV by leaf agroinfiltration of combinations of agrobacteria carrying full-length cDNA clones of BNYVV RNAs. We validated the ability of agroclones to reproduce a complete viral cycle, from replication to cell-to-cell and systemic movement and, finally, plant-to-plant transmission by its plasmodiophorid vector. We also showed successful root agroinfection of B. vulgaris, a new tool for the assay of resistance to rhizomania, the sugar beet disease caused by BNYVV. © 2013 BSPP AND BLACKWELL PUBLISHING LTD.

7 CFR 1435.306 - Allocation of marketing allotments to processors.

Code of Federal Regulations, 2013 CFR

2013-01-01

...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.306 Allocation of marketing allotments to processors. (a) Each sugar beet processor's allocation, other than a new entrant's, of the beet allotment will be...

7 CFR 1435.306 - Allocation of marketing allotments to processors.

Code of Federal Regulations, 2014 CFR

2014-01-01

...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.306 Allocation of marketing allotments to processors. (a) Each sugar beet processor's allocation, other than a new entrant's, of the beet allotment will be...

7 CFR 1435.306 - Allocation of marketing allotments to processors.

Code of Federal Regulations, 2012 CFR

2012-01-01

...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.306 Allocation of marketing allotments to processors. (a) Each sugar beet processor's allocation, other than a new entrant's, of the beet allotment will be...

7 CFR 1435.306 - Allocation of marketing allotments to processors.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.306 Allocation of marketing allotments to processors. (a) Each sugar beet processor's allocation, other than a new entrant's, of the beet allotment will be...

7 CFR 1435.306 - Allocation of marketing allotments to processors.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS SUGAR PROGRAM Flexible Marketing Allotments For Sugar § 1435.306 Allocation of marketing allotments to processors. (a) Each sugar beet processor's allocation, other than a new entrant's, of the beet allotment will be...

Recovery Effects of Oral Administration of Glucosylceramide and Beet Extract on Skin Barrier Destruction by UVB in Hairless Mice.

PubMed

Tokudome, Yoshihiro; Masutani, Noriomi; Uchino, Shohei; Fukai, Hisano

2017-10-27

Purified glucosylceramide from beet extract (beet GlcCer) and beet extract containing an equal amount of GlcCer were administered orally to ultra violet B (UVB)-irradiated mice, and differences in the protective effects against skin barrier dysfunction caused by UVB irradiation were compared. In the beet GlcCer group, epidermal thickening and the decrease in stratum corneum (SC) ceramide content caused by UVB irradiation were reduced. In the group that was orally administered beet extract containing glucosylceramide, effects similar to those in the beet GlcCer group were observed. Oral administration of beet GlcCer had no obvious effects against an increase in TEWL or decrease in SC water content after UVB irradiation, but there was improvement in the beet extract group. Oral administration of beet GlcCer is effective in improving skin barrier function in UVB-irradiated mice. Beet extract contains constituents other than GlcCer that are also effective in improving skin barrier function.

Concept for Recycling Waste Biomass from the Sugar Industry for Chemical and Biotechnological Purposes.

PubMed

Modelska, Magdalena; Berlowska, Joanna; Kregiel, Dorota; Cieciura, Weronika; Antolak, Hubert; Tomaszewska, Jolanta; Binczarski, Michał; Szubiakiewicz, Elzbieta; Witonska, Izabela A

2017-09-13

The objective of this study was to develop a method for the thermally-assisted acidic hydrolysis of waste biomass from the sugar industry (sugar beet pulp and leaves) for chemical and biotechnological purposes. The distillates, containing furfural, can be catalytically reduced directly into furfurayl alcohol or tetrahydrofurfuryl alcohol. The sugars present in the hydrolysates can be converted by lactic bacteria into lactic acid, which, by catalytic reduction, leads to propylene glycol. The sugars may also be utilized by microorganisms in the process of cell proliferation, and the biomass obtained used as a protein supplement in animal feed. Our study also considered the effects of the mode and length of preservation (fresh, ensilage, and drying) on the yields of furfural and monosaccharides. The yield of furfural in the distillates was measured using gas chromatography with flame ionization detector (GC-FID). The content of monosaccharides in the hydrolysates was measured spectrophotometrically using enzymatic kits. Biomass preserved under all tested conditions produced high yields of furfural, comparable to those for fresh material. Long-term storage of ensiled waste biomass did not result in loss of furfural productivity. However, there were significant reductions in the amounts of monosaccharides in the hydrolysates.

8. August, 1971. SECOND FLOOR LOOKING NW. EVAPORATOR UNITS USED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. August, 1971. SECOND FLOOR LOOKING NW. EVAPORATOR UNITS USED IN SEQUENCE TO REDUCE OR CONCENTRATE BEET JUICE. - Utah Sugar Company, Garland Beet Sugar Refinery, Factory Street, Garland, Box Elder County, UT

Genetics and Genomics

USDA-ARS?s Scientific Manuscript database

Good progress is being made on genetics and genomics of sugar beet, however it is in process and the tools are now being generated and some results are being analyzed. The GABI BeetSeq project released a first draft of the sugar beet genome of KWS2320, a dihaploid (see http://bvseq.molgen.mpg.de/Gen...

77 FR 23625 - Quizalofop Ethyl; Pesticide Tolerances

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-20

... via the oral, dermal, and inhalation routes of exposure. It is not an eye or dermal irritant nor a... the correct terminology, as follows: Bean, dry to bean, dry seed; sorghum, grain to sorghum, grain... 0.05 Bean, dry, seed 0.4 Bean, succulent 0.25 Beet, sugar, molasses 0.2 Beet, sugar, roots 0.1 Beet...

The effect of yeast (Saccharomyces cerevisiae) on nutrient intake, digestibility and finishing performance of lambs fed a diet based on dried molasses sugar beet-pulp.

PubMed

Payandeh, S; Kafilzadeh, F

2007-12-15

This experiment was conducted to determine the effect of yeast (Saccharomyces cerevisiae, SC47) on finishing performance, digestibility, some blood metabolites and carcass characteristics of male lambs fed a diet based on dried Molasses Sugar Beet-Pulp (MSBP). Eighteen Sanjabi male lambs (20.95 +/- 2.7 kg initial body weight and 3 month of age) were used in a completely randomized design. Animals were assigned to one of the two dietary treatments (with or without yeast). Digestibility and nitrogen balance experiment was carried out using six mature rams on finishing diet with and without yeast. Serum metabolites were determined in samples taken from lambs at the end of finishing period. Dry matter digestibility of finishing diet was significantly increased by yeast addition. However, yeast did not have any significant effect on apparent digestibility of OM, NDF, CP and energy. Nitrogen retention was also not affected by yeast addition. Yeast resulted in a significant increase in the average daily gain, dry matter and organic matter intake. However, feed conversion ratio was not significantly affected by addition of yeast. The concentration of the serum metabolites including glucose, urea, cholesterol, sodium, potassium, calcium, phosphorous and cratinine were not affected significantly by yeast supplementation, but triglyceride concentrations increased significantly when yeast was fed. Addition of yeast to the diet did not have any significant effect on the carcass characteristics. Results of this study suggest that feeding saccharomyces cerevisiae with a diet based on MSBP can improve the performance of fattening lambs without any change in carcass characteristics or cuts.

Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran

PubMed Central

Anabestani, Ameneh; Behjatnia, Seyed Ali Akbar; Izadpanah, Keramat; Tabein, Saeid

2017-01-01

Beet curly top virus (BCTV) and beet curly top Iran virus (BCTIV) are known as the causal agents of curly top disease in beet and several other dicotyledonous plants in Iran. These viruses are transmitted by Circulifer species, and until now, there has been no confirmed report of their seed transmission. A percentage (38.2–78.0%) of the seedlings developed from the seeds of a petunia local cultivar under insect-free conditions showed stunting, interveinal chlorosis, leaf curling, and vein swelling symptoms, and were infected by BCTV when tested by PCR. Presence of BCTV in seed extracts of petunia local cultivar was confirmed by PCR and IC-PCR, followed by sequencing. Agroinoculation of curly top free petunia plants with a BCTV infectious clone resulted in BCTV infection of plants and their developed seeds. These results show the seed infection and transmission of BCTV in a local cultivar of petunia. Similar experiments performed with BCTIV showed that this virus is also seed transmissible in the same cultivar of petunia, although with a lower rate (8.8–18.5%). Seed transmission of curly top viruses may have significant implications in the epidemiology of these viruses. PMID:29035342

29 CFR 780.804 - “Ginning” of cotton.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false âGinningâ of cotton. 780.804 Section 780.804 Labor... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for...

29 CFR 780.805 - Ginning of “cotton.”

Code of Federal Regulations, 2014 CFR

2014-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for Market § 780.805 Ginning of “cotton.” Only the ginning of “cotton” is within the first part of the...

29 CFR 780.804 - “Ginning” of cotton.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false âGinningâ of cotton. 780.804 Section 780.804 Labor... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for...

29 CFR 780.804 - “Ginning” of cotton.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false âGinningâ of cotton. 780.804 Section 780.804 Labor... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for...

29 CFR 780.805 - Ginning of “cotton.”

Code of Federal Regulations, 2011 CFR

2011-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for Market § 780.805 Ginning of “cotton.” Only the ginning of “cotton” is within the first part of the...

29 CFR 780.805 - Ginning of “cotton.”

Code of Federal Regulations, 2013 CFR

2013-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for Market § 780.805 Ginning of “cotton.” Only the ginning of “cotton” is within the first part of the...

29 CFR 780.804 - “Ginning” of cotton.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false âGinningâ of cotton. 780.804 Section 780.804 Labor... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for...

29 CFR 780.804 - “Ginning” of cotton.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false âGinningâ of cotton. 780.804 Section 780.804 Labor... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for...

29 CFR 780.805 - Ginning of “cotton.”

Code of Federal Regulations, 2012 CFR

2012-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for Market § 780.805 Ginning of “cotton.” Only the ginning of “cotton” is within the first part of the...

29 CFR 780.805 - Ginning of “cotton.”

Code of Federal Regulations, 2010 CFR

2010-07-01

... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for Market § 780.805 Ginning of “cotton.” Only the ginning of “cotton” is within the first part of the...

Photoacoustic and optothermal studies of tomato ketchup adulterated by the red beet (Beta vulgaris)

NASA Astrophysics Data System (ADS)

Bicanic, D.; Westra, E.; Seters, J.; van Houten, S.; Huberts, D.; Colić-Barić, I.; Cozijnsen, J.; Boshoven, H.

2005-06-01

Photoacoustic (PA) spectroscopy and optothermal window (OW) technique were used to explore their potential to detect red beet added as a colorant to tomato ketchup. The associated changes of colour resulting in the changes of absorbance (and hence of PA and OT signals) were monitored in the 500 nm region corresponding to the absorption maximum of lycopene. Both methods were shown capable of quantifying about 1% of red beet (by mass) in the mixture of ketchup and red beet.

40 CFR 180.206 - Phorate; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... following food commodities: Commodity Parts per million Bean, dry, seed 0.05 Bean, succulent 0.05 Beet, sugar, roots 0.3 Beet, sugar, tops 3.0 Coffee, green bean 1 0.02 Corn, field, forage 0.5 Corn, field...

40 CFR 180.206 - Phorate; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

... following food commodities: Commodity Parts per million Bean, dry, seed 0.05 Bean, succulent 0.05 Beet, sugar, roots 0.3 Beet, sugar, tops 3.0 Coffee, green bean 1 0.02 Corn, field, forage 0.5 Corn, field...

40 CFR 180.206 - Phorate; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... following food commodities: Commodity Parts per million Bean, dry, seed 0.05 Bean, succulent 0.05 Beet, sugar, roots 0.3 Beet, sugar, tops 3.0 Coffee, green bean 1 0.02 Corn, field, forage 0.5 Corn, field...

40 CFR 180.206 - Phorate; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... following food commodities: Commodity Parts per million Bean, dry, seed 0.05 Bean, succulent 0.05 Beet, sugar, roots 0.3 Beet, sugar, tops 3.0 Coffee, green bean 1 0.02 Corn, field, forage 0.5 Corn, field...

40 CFR 180.206 - Phorate; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... following food commodities: Commodity Parts per million Bean, dry, seed 0.05 Bean, succulent 0.05 Beet, sugar, roots 0.3 Beet, sugar, tops 3.0 Coffee, green bean 1 0.02 Corn, field, forage 0.5 Corn, field...

Effects of Pre-Converted Nitrite from Red Beet and Ascorbic Acid on Quality Characteristics in Meat Emulsions

PubMed Central

Kim, Hyun-Wook; Hwang, Ko-Eun

2017-01-01

We investigated the effects of fermented red beet extract and ascorbic acid on color development in meat emulsions. The pH of meat emulsions containing red beet extract decreased with an increase in the amount of extract added. The redness of the treated meat emulsions was higher than that of the control with no added nitrite or fermented red beet extract (p<0.05), though the redness of the meat emulsions treated with fermented red beet extract only was lower than in that treated with both fermented red beet extract and ascorbic acid (p<0.05). The highest VBN, TBARS, and total viable count values were observed in the control, and these values in the meat emulsions treated with fermented red beet extract were higher than in that treated with both fermented red beet extract and ascorbic acid (p<0.05). E. coli and coliform bacteria were not found in any of the meat emulsions tested. Treatment T2, containing nitrite and ascorbic acid, had the highest overall acceptability score (p<0.05); however, there was no significant difference between the T2 treatment and the T6 treatment, which contained 10% pre-converted nitrite from red beet extract and 0.05% ascorbic acid (p>0.05). The residual nitrite content of the meat emulsions treated with ascorbic acid was lower than in those treated without ascorbic acid (p<0.05). Thus, the combination of fermented red beet extract and ascorbic acid could be a viable alternative to synthetic nitrite for the stability of color development in meat emulsions. PMID:28515652

Effects of Pre-Converted Nitrite from Red Beet and Ascorbic Acid on Quality Characteristics in Meat Emulsions.

PubMed

Choi, Yun-Sang; Kim, Tae-Kyung; Jeon, Ki-Hong; Park, Jong-Dae; Kim, Hyun-Wook; Hwang, Ko-Eun; Kim, Young-Boong

2017-01-01

We investigated the effects of fermented red beet extract and ascorbic acid on color development in meat emulsions. The pH of meat emulsions containing red beet extract decreased with an increase in the amount of extract added. The redness of the treated meat emulsions was higher than that of the control with no added nitrite or fermented red beet extract ( p <0.05), though the redness of the meat emulsions treated with fermented red beet extract only was lower than in that treated with both fermented red beet extract and ascorbic acid ( p <0.05). The highest VBN, TBARS, and total viable count values were observed in the control, and these values in the meat emulsions treated with fermented red beet extract were higher than in that treated with both fermented red beet extract and ascorbic acid ( p <0.05). E. coli and coliform bacteria were not found in any of the meat emulsions tested. Treatment T2, containing nitrite and ascorbic acid, had the highest overall acceptability score ( p <0.05); however, there was no significant difference between the T2 treatment and the T6 treatment, which contained 10% pre-converted nitrite from red beet extract and 0.05% ascorbic acid ( p >0.05). The residual nitrite content of the meat emulsions treated with ascorbic acid was lower than in those treated without ascorbic acid ( p <0.05). Thus, the combination of fermented red beet extract and ascorbic acid could be a viable alternative to synthetic nitrite for the stability of color development in meat emulsions.

29 CFR 780.807 - Cotton must be ginned “for market.”

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false Cotton must be ginned âfor market.â 780.807 Section 780.807... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton...

29 CFR 780.807 - Cotton must be ginned “for market.”

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false Cotton must be ginned âfor market.â 780.807 Section 780.807... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton...

29 CFR 780.807 - Cotton must be ginned “for market.”

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false Cotton must be ginned âfor market.â 780.807 Section 780.807... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton...

29 CFR 780.807 - Cotton must be ginned “for market.”

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Cotton must be ginned âfor market.â 780.807 Section 780.807... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton...

29 CFR 780.807 - Cotton must be ginned “for market.”

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false Cotton must be ginned âfor market.â 780.807 Section 780.807... Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton...

40 CFR 180.269 - Aldicarb; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... commodities: Commodity Parts per million Bean, dry, seed 0.1 Beet, sugar, roots 0.05 Beet, sugar, tops 1 Citrus, dried pulp 0.6 Coffee, bean, green 0.1 Cotton, undelinted seed 0.1 Cotton, hulls 0.3 Grapefruit 0...

40 CFR 180.269 - Aldicarb; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

... commodities: Commodity Parts per million Bean, dry, seed 0.1 Beet, sugar, roots 0.05 Beet, sugar, tops 1 Citrus, dried pulp 0.6 Coffee, bean, green 0.1 Cotton, undelinted seed 0.1 Cotton, hulls 0.3 Grapefruit 0...

40 CFR 180.269 - Aldicarb; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... commodities: Commodity Parts per million Bean, dry, seed 0.1 Beet, sugar, roots 0.05 Beet, sugar, tops 1 Citrus, dried pulp 0.6 Coffee, bean, green 0.1 Cotton, undelinted seed 0.1 Cotton, hulls 0.3 Grapefruit 0...

40 CFR 180.269 - Aldicarb; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... commodities: Commodity Parts per million Bean, dry, seed 0.1 Beet, sugar, roots 0.05 Beet, sugar, tops 1 Citrus, dried pulp 0.6 Coffee, bean, green 0.1 Cotton, undelinted seed 0.1 Cotton, hulls 0.3 Grapefruit 0...

40 CFR 180.269 - Aldicarb; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... commodities: Commodity Parts per million Bean, dry, seed 0.1 Beet, sugar, roots 0.05 Beet, sugar, tops 1 Citrus, dried pulp 0.6 Coffee, bean, green 0.1 Cotton, undelinted seed 0.1 Cotton, hulls 0.3 Grapefruit 0...

40 CFR 180.330 - S-(2-(Ethylsulfinyl)ethyl) O,O-dimethyl phosphorothioate; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., forage 5.0 Alfalfa, hay 11.0 Bean, lima 0.2 Beet, sugar, roots 0.3 Beet, sugar, tops 0.5 Broccoli 1.0... sulfone in or on the following food commodities: Commodity Parts per million Broccoli raab 2.0 (d...

A comparative study of the apparent total tract digestibility of carbohydrates in Icelandic and Danish warmblood horses fed two different haylages and a concentrate consisting of sugar beet pulp and black oats.

PubMed

Jensen, Rasmus Bovbjerg; Brokner, Christine; Knudsen, Knud Erik Bach; Tauson, Anne-Helene

2010-10-01

Four Icelandic (ICE) and four Danish Warmblood (DW) horses were used in a crossover study with two treatments to investigate the effect of breed and the effect of stage of maturity of haylage on the apparent total tract digestibility (ATTD) of a diet consisting of sugar beet pulp, black oats and haylage early or late cut. Fibre was analysed as crude fibre (CF), acid detergent fibre (ADF), neutral detergent fibre (NDF) and dietary fibre (DF = non-starch polysaccharides (NSP) plus lignin). In haylage all analysed fibre fractions increased with advancing stage of maturity, with the cell wall components cellulose, non-cellulosic residue, xylose and lignin causing this increase. Crude protein (CP) and sugars decreased with advancing stage of maturity. Feeding early cut haylage resulted in a significantly (p < 0.05) higher ATTD of dry matter (DM), organic matter (OM), energy, NDF, total NSP, DF and CP compared to feeding late cut haylage. There was a significantly (p < 0.05) higher ATTD of CF, DF and starch in ICE than in DW. Diet affected several faecal parameters with a significantly (p < 0.05) lower pH in faeces when horses were fed the early cut haylage. Concentrations of total short-chain fatty acids were significantly (p < 0.05) higher in faeces from horses fed early cut haylage, reflecting the higher fermentability (higher ATTD) of this diet. There was no marked effect of breed on faecal parameters. The DF analysis method gave the most appropriate differentiation of the fibre fractions and their digestibility, compared to the traditional CF, ADF and NDF analyses. A major advantage of the DF analysis is the capacity of recovering soluble fibres. The results suggested that ICE had higher ATTD of DF than DW, and this was caused by a tendency for a higher ATTD of cellulose, but further studies are required to verify that in general.

Beta vulgaris crop types: Genomic signatures of selection (GSS) using next generation sequencing of pooled samples

USDA-ARS?s Scientific Manuscript database

Beta vulgaris crop types represent highly diverged populations with distinct phenotypes resulting from long-term selection. Differential end use in the crop types includes: leaf quality (chard/leaf beet), root enlargement and biomass, (table beet, fodder beet, sugar beet), and secondary metabolite a...

Genetic Variation of Beet Armyworm (Lepidoptera: Noctuidae) Populations Detected Using Microsatellite Markers in Iran.

PubMed

Golikhajeh, Neshat; Naseri, Bahram; Razmjou, Jabraeil; Hosseini, Reza; Aghbolaghi, Marzieh Asadi

2018-05-28

In order to understand the population genetic diversity and structure of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), a serious pest of sugar beet in Iran and the world, we genotyped 133 individuals from seven regions in Iran using four microsatellite loci. Significant difference was seen between the observed and expected heterozygosity in all loci. A lower observed heterozygosity than expected heterozygosity indicated a low heterozygosity in these populations. The value of F showed a high genetic differentiation, so that the mean of Fst was 0.21. Molecular analysis variance showed significant differences within and among populations with group variance accounted for 71 and 21%, respectively. No correlation was found between pair-wise Fst and geographic distance by Mantel test. Bayesian clustering analysis grouped all regions to two clusters. These data suggested that a combination of different factors, such as geographic distance, environmental condition, and physiological behavior in addition to genetic factors, could play an important role in forming variation within and between S. exigua populations.

40 CFR 180.1087 - Sesame stalks; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD... exemption from the requirement of a tolerance is established for residues of the biorational nematicide... fractions; potato; beet, sugar, roots; beet, sugar, tops; tomato; pepper, bell; squash; strawberry; eggplant...

40 CFR 180.1087 - Sesame stalks; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD... exemption from the requirement of a tolerance is established for residues of the biorational nematicide... fractions; potato; beet, sugar, roots; beet, sugar, tops; tomato; pepper, bell; squash; strawberry; eggplant...

40 CFR 180.257 - Chloroneb; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., in or on the commodity. Commodity Parts permillion Expiration/revocation date Bean, dry, seed 0.2 4/16/12 Bean, succulent 0.2 4/16/12 Beet, sugar, roots 0.2 4/16/12 Beet, sugar, tops 0.2 4/16/12 Cowpea...

40 CFR 180.668 - Sulfoxaflor; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Parts per million Almond, hulls 6.0 Barley, grain 0.40 Barley, hay 1.0 Barley, straw 2.0 Bean, dry seed 0.20 Bean, succulent 4.0 Beet, sugar, dried pulp 0.07 Beet, sugar, molasses 0.25 Berry, low growing...

40 CFR 180.257 - Chloroneb; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., in or on the commodity. Commodity Parts permillion Expiration/revocation date Bean, dry, seed 0.2 4/16/12 Bean, succulent 0.2 4/16/12 Beet, sugar, roots 0.2 4/16/12 Beet, sugar, tops 0.2 4/16/12 Cowpea...

40 CFR 180.257 - Chloroneb; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., in or on the commodity. Commodity Parts permillion Expiration/revocation date Bean, dry, seed 0.2 4/16/12 Bean, succulent 0.2 4/16/12 Beet, sugar, roots 0.2 4/16/12 Beet, sugar, tops 0.2 4/16/12 Cowpea...

40 CFR 180.668 - Sulfoxaflor; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Parts per million Almond, hulls 6.0 Barley, grain 0.40 Barley, hay 1.0 Barley, straw 2.0 Bean, dry seed 0.20 Bean, succulent 4.0 Beet, sugar, dried pulp 0.07 Beet, sugar, molasses 0.25 Berry, low growing...

40 CFR 180.475 - Difenoconazole; tolerances for residues.