Measurement of Sediment Deposition Rates using an Optical Backscatter Sensor
NASA Astrophysics Data System (ADS)
Ridd, P.; Day, G.; Thomas, S.; Harradence, J.; Fox, D.; Bunt, J.; Renagi, O.; Jago, C.
2001-02-01
An optical method for measuring siltation of sediment has been developed using an optical fibre backscatter (OBS) nephelometer. Sediment settling upon the optical fibre sensor causes an increase in the backscatter reading which can be related to the settled sediment surface density (SSSD) as measured in units of mg cm -2. Calibration and laboratory tests indicate that the resolution of measurements of SSSD is 0·01 mg cm -2and an accuracy of 5% in still water. In moving water it is more difficult to determine the accuracy of the method because other methods with suitable resolution are unavailable. However, indirect methods using measurements of changing suspended sediment concentration in a ring flume, indicate that the OBS method under-predicts deposition. The series of siltation from three field sites are presented. This sensor offers considerable advances over other methods of measuring settling because time series of settling may be taken and thus settling events may be related to other hydrodynamic parameters such as wave climate and currents.
NASA Astrophysics Data System (ADS)
Xu, K.; Champagne, B. N.
2017-12-01
The transport of sediment in the coastal zone and continental shelf is highly impacted by fluvial and oceanographic dynamics. In Louisiana, the Mississippi River delivers a bulk of water, sediment, and nutrients to the coast. However, coastal land loss highlights the importance of the sediment deposited at the mouth of the river. Sediment is the foundation to build land and suspended sediment concentration (SSC) tracks the delivery, deposition, and erosion of sediment. On a more applicable scale, variables such as SSC can be used to calculate sediment transport flux, an important parameter for projects such as sediment diversions and barrier island restoration. In order to rely on suspended sediment concentration (SSC) as continuous data, lab experiments are needed to establish the relationship between turbidity and SSC. Factors such as sensor type (optical or acoustic) and grain size (coarse or fine) can greatly impact the estimated SSC. In this study, fine-grained sediment was collected from multiple sites in coastal Louisiana and used to calibrate both optical backscatter (OBS) and acoustic backscatter (ABS) sensors to establish the relationship between sensor type and accuracy of the SSC estimation. Multiple grain-size analyses using a Laser Diffraction Particle Size Analyzer helped determine the effects of sensor accuracy regarding grain size. The results of these experiments were combined in order to establish the calibration curves of SSC. Our results indicated that the OBS-3A sensor's turbidity data were more correlated with the SSC than the OBS-5+'s data. Possible explanations for this could be due to differences between the instruments' measuring ranges and their sensitivity to various grain sizes. This technology development has a broad impact to the studies of sediment delivery, transport, and deposition in multiple types of coastal protection and restoration projects.
Biological interference of optical backscatterance sensors in Tampa Bay, Florida
Schoellhamer, D.H.
1993-01-01
Optical backscatterance (OBS, D&A Instruments, Inc.1 1 Use of brand, firm, or trade names in this paper is for identification purposes only and does not constitute endorsement by the U.S. Geological Survey.) sensors for measuring suspended-solids concentrations have been deployed in Tampa Bay to monitor resuspension of bottom sediments. This paper describes biological factors that affected the OBS sensors deployed in Tampa Bay and discusses deployment strategies that minimize biological interference. Phytoplankton may interfere with the OBS sensors when the suspended-solids concentration is near or below the sensor response threshold. Fish swimming in front of the OBS sensors caused spikes in the OBS sensor output, so the median average was more appropriate than the mean average. An algal slime on the OBS sensors caused excessive backscatterance that dominated the backscatterance from suspended material. Because of the fouling problem, deployments were limited to less than a week, and OBS sensors were cleaned daily, if possible. Calibration of OBS sensors with water samples collected from Tampa Bay was satisfactory when biological interference was not significant. When properly deployed, the OBS sensors can successfully monitor sediment resuspension in Tampa Bay and similar subtropical estuaries. ?? 1993.
NASA Astrophysics Data System (ADS)
Ierodiaconou, Daniel; Schimel, Alexandre C. G.; Kennedy, David; Monk, Jacquomo; Gaylard, Grace; Young, Mary; Diesing, Markus; Rattray, Alex
2018-06-01
Habitat mapping data are increasingly being recognised for their importance in underpinning marine spatial planning. The ability to collect ultra-high resolution (cm) multibeam echosounder (MBES) data in shallow waters has facilitated understanding of the fine-scale distribution of benthic habitats in these areas that are often prone to human disturbance. Developing quantitative and objective approaches to integrate MBES data with ground observations for predictive modelling is essential for ensuring repeatability and providing confidence measures for habitat mapping products. Whilst supervised classification approaches are becoming more common, users are often faced with a decision whether to implement a pixel based (PB) or an object based (OB) image analysis approach, with often limited understanding of the potential influence of that decision on final map products and relative importance of data inputs to patterns observed. In this study, we apply an ensemble learning approach capable of integrating PB and OB Image Analysis from ultra-high resolution MBES bathymetry and backscatter data for mapping benthic habitats in Refuge Cove, a temperate coastal embayment in south-east Australia. We demonstrate the relative importance of PB and OB seafloor derivatives for the five broad benthic habitats that dominate the site. We found that OB and PB approaches performed well with differences in classification accuracy but not discernible statistically. However, a model incorporating elements of both approaches proved to be significantly more accurate than OB or PB methods alone and demonstrate the benefits of using MBES bathymetry and backscatter combined for class discrimination.
NASA Astrophysics Data System (ADS)
Lynch, J. F.; Gross, T. F.; Sherwood, C. R.; Irish, J. D.; Brumley, B. H.
1997-04-01
During the 1988-1989 Sediment Transport Events on Shelves and Slopes (STRESS) experiment, a 1-MHz acoustic backscatter system (ABSS), deployed in 90 m of water off the California coast measured vertical profiles of suspended sediment concentration from 1.5 to (nominally) 26 meters above bottom (m.a.b.). An 8-week-long time series was obtained, showing major sediment transport events (storms) in late December and early January. Comparison of the acoustics measurements from 1.5 m.a.b. are made with optical backscatter system (OBS) concentration estimates lower in the boundary layer (0.25 m.a.b.). Correlations between ABSS and OBS concentration measurements and the boundary layer forcing functions (waves, currents, and their non-linear interaction) provided a variety of insights into the nature of the sediment transport of the STRESS site. Transport rates and integrated transport are seen to be dominated by the largest storm events.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Berdeyans, D.; Bocharov, V.I.; Lobachevskii, L.A.
Ionosphere observations by the OBS method were performed to study ionospheric conditions under which radio waves in the decameter range propagate on Cuba--Soviet Union paths. The results of observations in the summer of 1973 are reported. The distance--frequency and distance--time characteristics of back-scattered signals in the sounding direction for each day of observation are discussed. (JFP)
2012-09-30
standard linear wave theory. Suspended sediment concentration (SSC) was estimated using the backscatter signal of the ADCP and the turbidity value...measured by the OBS when present. The OBS turbidity signal was calibrated against SSC measured in a laboratory tank, using sediments collected on the...link the geotechnical properties of sediment substrates to the spatial and hydrodynamic characteristics of tidal channels • To develop new
Noninvasive identification of bladder cancer with subsurface backscattered light
NASA Astrophysics Data System (ADS)
Bigio, Irving J.; Mourant, Judith R.; Boyer, James D.; Johnson, Tamara M.; Shimada, Tsutomu; Conn, Richard L.
1994-05-01
We have developed and are testing early prototypes of an optical biopsy system (OBS) for detection of cancer and other tissue pathologies. The OBS invokes a unique approach to optical diagnosis of tissue pathologies based on the elastic scattering properties, over a wide range of wavelengths, of the microscopic structure of the tissue. Absorption bands in the tissue also add useful complexity to the spectral data collected. The data acquisition and storage/display time with the OBS instrument is approximately 1 second. Thus, in addition to the reduced invasiveness of this technique compared with current state-of- the-art methods, the OBS offers the possibility of impressively faster diagnostic assessment. The OBS employs a small fiber-optic probe that is amendable to use with any endoscope, catheter or hypodermic, or to direct surface examination (e.g., as in skin cancer or cervical cancer). We report here specifically on its potential application in the detection of bladder cancer.
Data files from the Grays Harbor Sediment Transport Experiment Spring 2001
Landerman, Laura A.; Sherwood, Christopher R.; Gelfenbaum, Guy; Lacy, Jessica; Ruggiero, Peter; Wilson, Douglas; Chisholm, Tom; Kurrus, Keith
2005-01-01
This publication consists of two DVD-ROMs, both of which are presented here. This report describes data collected during the Spring 2001 Grays Harbor Sediment Transport Experiment, and provides additional information needed to interpret the data. Two DVDs accompany this report; both contain documentation in html format that assist the user in navigating through the data. DVD-ROM-1 contains a digital version of this report in .pdf format, raw Aquatec acoustic backscatter (ABS) data in .zip format, Sonar data files in .avi format, and coastal processes and morphology data in ASCII format. ASCII data files are provided in .zip format; bundled coastal processes ASCII files are separated by deployment and instrument; bundled morphology ASCII files are separated into monthly data collection efforts containing the beach profiles collected (or extracted from the surface map) at that time; weekly surface maps are also bundled together. DVD-ROM-2 contains a digital version of this report in .pdf format, the binary data files collected by the SonTek instrumentation, calibration files for the pressure sensors, and Matlab m-files for loading the ABS data into Matlab and cleaning-up the optical backscatter (OBS) burst time-series data.
Holographic otoscope for nano-displacement measurements of surfaces under dynamic excitation
Flores-Moreno, J. M.; Furlong, Cosme; Rosowski, John J.; Harrington, Ellery; Cheng, Jeffrey T.; Scarpino, C.; Santoyo, F. Mendoza
2011-01-01
Summary We describe a novel holographic otoscope system for measuring nano-displacements of objects subjected to dynamic excitation. Such measurements are necessary to quantify the mechanical deformation of surfaces in mechanics, acoustics, electronics, biology and many other fields. In particular, we are interested in measuring the sound-induced motion of biological samples, such as an eardrum. Our holographic otoscope system consists of laser illumination delivery (IS), optical head (OH), and image processing computer (IP) systems. The IS delivers the object beam (OB) and the reference beam (RB) to the OH. The backscattered light coming from the object illuminated by the OB interferes with the RB at the camera sensor plane to be digitally recorded as a hologram. The hologram is processed by the IP using Fresnel numerical reconstruction algorithm, where the focal plane can be selected freely. Our holographic otoscope system is currently deployed in a clinic, and is packaged in a custom design. It is mounted in a mechatronic positioning system to increase its maneuverability degrees to be conveniently positioned in front of the object to be measured. We present representative results highlighting the versatility of our system to measure deformations of complex elastic surfaces in the wavelength scale including a copper foil membrane and postmortem tympanic membrane (TM). PMID:21898459
Holographic otoscope for nanodisplacement measurements of surfaces under dynamic excitation.
Flores-Moreno, J M; Furlong, Cosme; Rosowski, John J; Harrington, Ellery; Cheng, Jeffrey T; Scarpino, C; Santoyo, F Mendoza
2011-01-01
We describe a novel holographic otoscope system for measuring nanodisplacements of objects subjected to dynamic excitation. Such measurements are necessary to quantify the mechanical deformation of surfaces in mechanics, acoustics, electronics, biology, and many other fields. In particular, we are interested in measuring the sound-induced motion of biological samples, such as an eardrum. Our holographic otoscope system consists of laser illumination delivery (IS), optical head (OH), and image processing computer (IP) systems. The IS delivers the object beam (OB) and the reference beam (RB) to the OH. The backscattered light coming from the object illuminated by the OB interferes with the RB at the camera sensor plane to be digitally recorded as a hologram. The hologram is processed by the IP using the Fresnel numerical reconstruction algorithm, where the focal plane can be selected freely. Our holographic otoscope system is currently deployed in a clinic, and is packaged in a custom design. It is mounted in a mechatronic positioning system to increase its maneuverability degrees to be conveniently positioned in front of the object to be measured. We present representative results highlighting the versatility of our system to measure deformations of complex elastic surfaces in the wavelength scale including a copper foil membrane and postmortem tympanic membrane. SCANNING 33: 342-352, 2011. © 2011 Wiley Periodicals, Inc. Copyright © 2011 Wiley Periodicals, Inc.
NASA Astrophysics Data System (ADS)
Hello, Y.; Yegikyan, M.; Charvis, P.; Philippe, O.
2017-12-01
Manta is a new BroadBand OBS developed at Geoazur and commercialized by Osean. The design is inspired by 3-years autonomy MUG-OBS a Multiparameter Ocean Bottom System which carry a lot of sort of sensor types. As Mug-OBS, Manta-OBS rated 6000m is designed to resist a trawling. All the components are non corrosive such polyethylene, titanium and buoyancy is ensured by syntactic foam. Equipped in standard version with a Trillium compact OBS Manta has an autonomy of 18 months, but can accept on its 4 input channels any kind of signal as low as from an hydrophone or larger from other type of a seismometer or accelerometer. Tri-axial geophones unit (2 Hz or 4.5 Hz ) can replace the seismometer and will expend the lifespan for the instrument. The seismometer is encapsulated in a central well established by four panels of the main structure to protect it from sea current convection and is decoupled from main chassis. An health bulletin is recoverable by acoustic any time to facilitate the installation and during a visit when instrument is deployed. Main parameters for acquisition can be changed by acoustics command from surface at any time. Once at the bottom, release for the main sensor installation is programmed on a timer but controlled by the tilt of the OBS. If the tilt is too important based on programmed limits, sensor will not released automatically, but this can be forced by acoustic command after returning the tilt informations to the boat operator. Manta is equipped with flash light and AIS system for easy location at recovery, and can also send it's position by Iridium satellite in case of an unexpected ascent such caused by a possible trawling if deployed in shallow water. Clock drift calculation is automatically made against GPS time signal once the OBS return at the surface. The recovery of the OBS is initiated by an acoustic command. These new features made Manta a very versatile instrument for monitoring earthquakes.
Weng, Shenglin; Li, Yiping; Wei, Jin; Du, Wei; Gao, Xiaomeng; Wang, Wencai; Wang, Jianwei; Acharya, Kumud; Luo, Liancong
2018-05-01
The identification of coherent structures is very important in investigating the sediment transport mechanism and controlling the eutrophication in shallow lakes. This study analyzed the turbulence characteristics and the sensitivity of quadrant analysis to threshold level. Simultaneous in situ measurements of velocities and suspended sediment concentration (SSC) were conducted in Lake Taihu with acoustic Doppler velocimeter (ADV) and optical backscatter sensor (OBS) instruments. The results show that the increase in hole size makes the difference between dominant and non-dominant events more distinct. Wind velocity determines the frequency of occurrence of sweep and ejection events, which provide dominant contributions to the Reynolds stress. The increase of wind velocity enlarges the magnitude of coherent events but has little impact on the events frequency with the same hole size. The events occurring within short periods provide large contributions to the momentum flux. Transportation and diffusion of sediment are in control of the intermittent coherent events to a large extent.
Kinematic analysis of conically scanned environmental properties
NASA Technical Reports Server (NTRS)
Wilkerson, Thomas D. (Inventor); Sanders, Jason A. (Inventor); Andrus, Ionio Q. (Inventor)
2003-01-01
A method for determining the velocity of features such as wind. The method preferably includes producing sensor signals and projecting the sensor signals sequentially along lines lying on the surface of a cone. The sensor signals may be in the form of lidar, radar or sonar for example. As the sensor signals are transmitted, the signals contact objects and are backscattered. The backscattered sensor signals are received to determine the location of objects as they pass through the transmission path. The speed and direction the object is moving may be calculated using the backscattered data. The data may be plotted in a two dimensional array with a scan angle on one axis and a scan time on the other axis. The prominent curves that appear in the plot may be analyzed to determine the speed and direction the object is traveling.
Moody, J. D.; Strozzi, D. J.; Divol, L.; ...
2013-07-09
Stimulated Raman backscatter is used as a remote sensor to quantify the instantaneous laser power after transfer from outer to inner cones that cross in a National Ignition Facility (NIF) gas-filled hohlraum plasma. By matching stimulated Raman backscatter between a shot reducing outer versus a shot reducing inner power we infer that about half of the incident outer-cone power is transferred to inner cones, for the specific time and wavelength configuration studied. Furthermore, this is the first instantaneous nondisruptive measure of power transfer in an indirect drive NIF experiment using optical measurements.
Laissez-Faire : Fully Asymmetric Backscatter Communication
Hu, Pan; Zhang, Pengyu; Ganesan, Deepak
2016-01-01
Backscatter provides dual-benefits of energy harvesting and low-power communication, making it attractive to a broad class of wireless sensors. But the design of a protocol that enables extremely power-efficient radios for harvesting-based sensors as well as high-rate data transfer for data-rich sensors presents a conundrum. In this paper, we present a new fully asymmetric backscatter communication protocol where nodes blindly transmit data as and when they sense. This model enables fully flexible node designs, from extraordinarily power-efficient backscatter radios that consume barely a few micro-watts to high-throughput radios that can stream at hundreds of Kbps while consuming a paltry tens of micro-watts. The challenge, however, lies in decoding concurrent streams at the reader, which we achieve using a novel combination of time-domain separation of interleaved signal edges, and phase-domain separation of colliding transmissions. We provide an implementation of our protocol, LF-Backscatter, and show that it can achieve an order of magnitude or more improvement in throughput, latency and power over state-of-art alternatives. PMID:28286885
NASA Astrophysics Data System (ADS)
Frontera, T.; Deschamps, A.; Ugalde, A.; Jara, J. A.; Hello, Y.; Goula, X.; Olivera, C.
2009-04-01
The aim of this work is to compare two permanent broad band ocean bottom seismometers (OBS) in terms of noise conditions by taking to account their similar instrumentation types, but their very different site characteristics. Both OBS stations have Güralp CMG-3T three component broad band sensors and differential pressure gauges (DPG). The first sensor operates since August 2005 under the framework of a research project that aims to improve the knowledge of the seismicity and seismic risk in the Tarragona region (north eastern Spain). This pioneering project in Spain is being carried out by the Institut Geològic de Catalunya (IGC) and the Observatori de l'Ebre, in collaboration with the Spanish oil company Repsol Investigaciones Petrolíferas, and is being financed by the Ministerio de Educación y Ciencia (CASABLANCA REN2003-06577), FEDER funding, the IGC and the Institut Cartogràfic de Catalunya. The OBS is installed inside the security perimeter of the Casablanca oil platform, which is located 40 km offshore Tarragona. The sensors are submerged at about 400 m to the SW of the oil platform at about 150 m in depth. Data are digitized on-site and are transmitted through a submarine cable to the platform, where they are recorded. In July 2007 some improvements were made: i) the OBS was completely buried and the DPG was moved about 10 m away from the OBS; and ii) via satellite signal transmission was implemented, which allowed to have continuous and real time data in Barcelona so that the OBS could be integrated into the Catalan Seismic Network. The second seismometer operates in the frame of the Antares neutrino telescope project developed in Liguria Sea. Geoazur is carrying out the project of deployment of a broad band seismological instrument in the aim of developing the permanent sea floor observation knowledge necessary to characterize Ligurian Sea seismicity and seismic risk along French Riviera coast (SE France). The operation was facilitated by the deployment of an opto-electrical cable to provide power and data transmission from the coast to a recording point at 2550 m in depth and 15km south to Porquerolles Island in the frame of the Antares neutrino telescope project. The program was founded in the 2002-2006 CPER (State-Region Research plan) by PACA region and CNRS/INSU and in the Antares collaboration which is responsible of all the marine operations on the site. The OBS and the DPG were set up by the ROV "Victor" from Ifremer (French Research Institute for Exploitation of the Sea) and partially buried; levelling and orientation were performed directly on the site. The instrument was running from April 2005 to April 2007. In April 2006 the sensor was completely buried. Data transmitted in quasi real time to Geoazur were integrated to the regional data center. In April 2007 the sensor was retrieved and reinstalled with data transmission protocol modification in October 2008. A water leak detected in December forced to cut the alimentation in January 2009. Because both sensors have not operated over the same time period, two different periods have been analysed: 2006 for the Antares OBS and 2008 for the Casablanca's one. In this way we are able to make a comparison of the noise behaviour by observing also the seasonal evolution. As observed on most of the ocean floor observatories, the noise is quite large on all components for both OBS. In general, the Antares OBS shows a better behaviour than the Casablanca OBS, due to the more favourable site conditions. Both OBS have been reinstalled to ameliorate some site condition deficiencies, which has basically affected the low frequency band, between 0.008 and 0.07 Hz. After the reinstallations, the noise conditions have improved at least 10 dB. A comparison of the power spectral density (PSD) curves for both stations concerning the periods after the reinstallations of the OBS show that, at low frequencies, the vertical component PSD curve for the Casablanca OBS is about 20 dB higher than the Antares PSD curve. However, both fall in between Peterson's new high (NHNM) and low (NLNM) noise models. The PSD of the horizontal components for the Casablanca OBS in the same frequency band is higher than that of the vertical component and is also higher than the NHNM. For the Antares OBS the PSD curves of the horizontal components are higher than the Antares vertical component PSD curve, but they lie inside the margins marked by Peterson's models. On the microseismic band, between 0.07 and 2 Hz, quieter PSD curves for the Antares OBS than for the Casablanca's one are observed. Nevertheless, the primary microseismic peak is not seen on the Antares PSD curves, whereas it can be observed on the vertical component PSD curve for the Casablanca OBS. As for the secondary microseimic peak, it is much better defined on the Antares PSD curves than for the Casablanca case for all components. Finally, at high frequencies between 2 and 50 Hz, high noise levels are observed for all components and both OBS.
Uncooperative target-in-the-loop performance with backscattered speckle-field effects
NASA Astrophysics Data System (ADS)
Kansky, Jan E.; Murphy, Daniel V.
2007-09-01
Systems utilizing target-in-the-loop (TIL) techniques for adaptive optics phase compensation rely on a metric sensor to perform a hill climbing algorithm that maximizes the far-field Strehl ratio. In uncooperative TIL, the metric signal is derived from the light backscattered from a target. In cases where the target is illuminated with a laser with suffciently long coherence length, the potential exists for the validity of the metric sensor to be compromised by speckle-field effects. We report experimental results from a scaled laboratory designed to evaluate TIL performance in atmospheric turbulence and thermal blooming conditions where the metric sensors are influenced by varying degrees of backscatter speckle. We compare performance of several TIL configurations and metrics for cases with static speckle, and for cases with speckle fluctuations within the frequency range that the TIL system operates. The roles of metric sensor filtering and system bandwidth are discussed.
Rodent wearable ultrasound system for wireless neural recording.
Piech, David K; Kay, Joshua E; Boser, Bernhard E; Maharbiz, Michel M
2017-07-01
Advances in minimally-invasive, distributed biological interface nodes enable possibilities for networks of sensors and actuators to connect the brain with external devices. The recent development of the neural dust sensor mote has shown that utilizing ultrasound backscatter communication enables untethered sub-mm neural recording devices. These implanted sensor motes require a wearable external ultrasound interrogation device to enable in-vivo, freely-behaving neural interface experiments. However, minimizing the complexity and size of the implanted sensors shifts the power and processing burden to the external interrogator. In this paper, we present an ultrasound backscatter interrogator that supports real-time backscatter processing in a rodent-wearable, completely wireless device. We demonstrate a generic digital encoding scheme which is intended for transmitting neural information. The system integrates a front-end ultrasonic interface ASIC with off-the-shelf components to enable a highly compact ultrasound interrogation device intended for rodent neural interface experiments but applicable to other model systems.
Frequency shift of the Bragg and Non-Bragg backscattering from periodic water wave
NASA Astrophysics Data System (ADS)
Wen, Biyang; Li, Ke
2016-08-01
Doppler effect is used to measure the relative speed of a moving target with respect to the radar, and is also used to interpret the frequency shift of the backscattering from the ocean wave according to the water-wave phase velocity. The widely known relationship between the Doppler shift and the water-wave phase velocity was deduced from the scattering measurements data collected from actual sea surface, and has not been verified under man-made conditions. Here we show that this ob- served frequency shift of the scattering data from the Bragg and Non-Bragg water wave is not the Doppler shift corresponding to the water-wave phase velocity as commonly believed, but is the water-wave frequency and its integral multiple frequency. The power spectrum of the backscatter from the periodic water wave consists of serials discrete peaks, which is equally spaced by water wave frequency. Only when the water-wave length is the integer multiples of the Bragg wave, and the radar range resolution is infinite, does the frequency shift of the backscattering mathematically equal the Doppler shift according to the water-wave phase velocity.
Noninvasive identification of bladder cancer with sub-surface backscattered light
DOE Office of Scientific and Technical Information (OSTI.GOV)
Bigio, I.J.; Mourant, J.R.; Boyer, J.
1994-02-01
A non-invasive diagnostic tool that could identify malignancy in situ and in real time would have a major impact on the detection and treatment of cancer. We have developed and are testing early prototypes of an optical biopsy system (OBS) for detection of cancer and other tissue pathologies. The OBS invokes a unique approach to optical diagnosis of tissue pathologies based on the elastic scattering properties, over a wide range of wavelengths, of the microscopic structure of the tissue. Absorption bands in the tissue also add useful complexity to the spectral data collected. The use of elastic scattering as themore » key to optical tissue diagnostics in the OBS is based on the fact that many tissue pathologies, including a majority of cancer forms, manifest significant architectural changes at the cellular and sub-cellular level. Since the cellular components that cause elastic scattering have dimensions typically on the order of visible to near-IR wavelengths, the elastic (Mie) scattering properties will be strongly wavelength dependent. Thus, morphology and size changes can be expected to cause significant changes in an optical signature that is derived from the wavelength-dependence of elastic scattering as well as absorption. The data acquisition and storage/display time with the OBS instrument is {approximately}1 second. Thus, in addition to the reduced invasiveness of this technique compared with current state-of-the-art methods (surgical biopsy and pathology analysis), the OBS offers the possibility of impressively faster diagnostic assessment. The OBS employs a small fiber-optic probe that is amenable to use with any endoscope, catheter or hypodermic, or to direct surface examination (e.g., as in skin cancer or cervical cancer). We report here specifically on its potential application in the detection of bladder cancer.« less
Yan, Aidong; Huang, Sheng; Li, Shuo; Chen, Rongzhang; Ohodnicki, Paul; Buric, Michael; Lee, Shiwoo; Li, Ming-Jun; Chen, Kevin P
2017-08-24
This paper reports a technique to enhance the magnitude and high-temperature stability of Rayleigh back-scattering signals in silica fibers for distributed sensing applications. With femtosecond laser radiation, more than 40-dB enhancement of Rayleigh backscattering signal was generated in silica fibers using 300-nJ laser pulses at 250 kHz repetition rate. The laser-induced Rayleigh scattering defects were found to be stable from the room temperature to 800 °C in hydrogen gas. The Rayleigh scatter at high temperatures was correlated to the formation and modification of nanogratings in the fiber core. Using optical fibers with enhanced Rayleigh backscattering profiles as distributed temperature sensors, we demonstrated real-time monitoring of solid oxide fuel cell (SOFC) operations with 5-mm spatial resolution at 800 °C. Information gathered by these fiber sensor tools can be used to verify simulation results or operated in a process-control system to improve the operational efficiency and longevity of SOFC-based energy generation systems.
Orienting Ocean Bottom Seismic Sensors from Ship Noise Polarization Analysis
NASA Astrophysics Data System (ADS)
Barruol, Guilhem; Dreo, Richard; Fontaine, Fabrice R.; Scholz, John R.; Sigloch, Karin; Geay, Bruno; Bouillon, Alexandre
2017-04-01
For the RHUM-RUM project (Réunion Hotspot and Upper Mantle - Réunions Unterer Mantel, www.rhum-rum.net), a network of 57 ocean-bottom seismometers (OBS) was installed on the ocean floor around La Réunion Island in the SW Indian Ocean. Part of the network happened to be located beneath a route of heavy ship traffic connecting SE-Asia and the South-Atlantic region. We analysed the ship noise recorded on the OBS and show that it can be used for determining the horizontal orientations of the seismic instruments as they were recording on the ocean floor. The OBS, provided by the German DEPAS and the French INSU OBS national pools, were equipped with wide-band or broad-band three-components seismic and hydro-acoustic sensors. They were deployed in Nov. 2012 by R/V Marion Dufresne and recovered by R/V Meteor one year later. Depending on the configuration, the OBS recorded for 8 to 13 months. By combining the trajectories of passing ships - provided by AIS (Automatic Identification system) GPS data - with our geophysical data recorded on the ocean floor, we show that both hydro-acoustic and seismic spectral analyses exhibit clear signals associated with vessels between 1 and 50 Hz, in the high-frequency range of our instruments. Large cargo vessels are detected several hours before and after their closest point of approach (CPA) and show clear Doppler effects which put quantitative constraints on their distances and speeds. By analysing the continuous noise polarization on the three seismic components, we show that the polarization of the noise emitted by ships passing in the neighbourhood of an ocean-bottom seismometer can be used for retrieving the orientation of the OBS horizontal components on the ocean floor with respect to the geographic reference frame. We find good agreement between OBS orientations thus calculated from ship noise and the OBS orientations determined independently from teleseismic body and surface wave polarization methods (Scholz et al., GJI, 2017).
NASA Astrophysics Data System (ADS)
Mi, Hongyu; Guan, Mingming; Liu, Jilin; Shan, Hongyan; Fei, Qiang; Huan, Yanfu; Feng, Guodong
2017-04-01
In this work, a turn on fluorescent sensor, based on Hg2 + coordination conjugated polymer, was developed to detect cysteine-containing compounds. The fluorescence of conjugated polymer (poly(2,5-bis (sodium 4-oxybutyrate) -1,4 - phenylethynylene-alt-1,4-phenyleneethynylene; PPE-OBS) would be quenched by Hg2 + because of the coordination-induced aggregation and electron transfers of PPE-OBS toward Hg2 +. When there were some cysteine-containing compounds in PPE-OBS-Hg2 + system, the fluorescence of PPE-OBS would be recovered. It indicated that the PPE-OBS-Hg2 + system could be used to detect cysteine-containing compounds. Under the optimized conditions, the experiment results showed that there were particularly linear range, high sensitivity and selectivity over other amino acids. The limit of detection (LOD) of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) were 0.725 μmol L- 1, 0.982 μmol L- 1 and 1.21 μmol L- 1 by using this sensor. In addition, Cys standard recovery in several green tea drink and honey samples was also demonstrated. The recovery of Cys was range from 96.3 to 105.0% and RSD was less than 3.25%. The satisfactory results demonstrated that the proposed method could be as a potential fluorescent method for determining cysteine-containing compounds in real samples.
Jürgens, Hella S; Schürmann, Annette; Kluge, Reinhart; Ortmann, Sylvia; Klaus, Susanne; Joost, Hans-Georg; Tschöp, Matthias H
2006-04-13
Among polygenic mouse models of obesity, the New Zealand obese (NZO) mouse exhibits the most severe phenotype, with fat depots exceeding 40% of total body weight at the age of 6 mo. Here we dissected the components of energy balance including feeding behavior, locomotor activity, energy expenditure, and thermogenesis compared with the related lean New Zealand black (NZB) and obese B6.V-Lep(ob)/J (ob/ob) strains (11% and 65% fat at 23 wk, respectively). NZO mice exhibited a significant hyperphagia that, when food intake was expressed per metabolic body mass, was less pronounced than that of the ob/ob strain. Compared with NZB, NZO mice exhibited increased meal frequency, meal duration, and meal size. Body temperature as determined by telemetry with implanted sensors was reduced in NZO mice, but again to a lesser extent than in the ob/ob strain. In striking contrast to ob/ob mice, NZO mice were able to maintain a constant body temperature during a 20-h cold exposure, thus exhibiting a functioning cold-induced thermogenesis. No significant differences in spontaneous home cage activity were observed among NZO, NZB, and ob/ob strains. When mice had access to voluntary running wheels, however, running activity was significantly lower in NZO than NZB mice and even lower in ob/ob mice. These data indicate that obesity in NZO mice, just as in humans, is due to a combination of hyperphagia, reduced energy expenditure, and insufficient physical activity. Because NZO mice differ strikingly from the ob/ob strain in their resistance to cold stress, we suggest that the molecular defects causing hyperphagia in NZO mice are located distal from leptin and its receptor.
Kishen, Anil; Shrestha, Annie; Rafique, Adeela
2008-01-01
In this study, a Fiber Optic Backscatter Spectroscopic Sensor (FOBSS) is used to monitor demineralization and remineralization induced changes in the enamel. A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface. The experiments were conducted in two parts. In Part 1, experiments were carried out using fiber optic backscatter spectroscopy on (1) sound enamel and dentine sections and (2) sound tooth specimens subjected to demineralization and remineralization. In Part 2, polarization microscopy was conducted to examine the depth of demineralization in tooth specimens. The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra. The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine. The experiments in Part 2 showed that demineralization and remineralization processes induced a linear decrease and linear increase in the backscatter light intensity respectively. A negative correlation between the decrease in the backscatter light intensity during demineralization and the depth of demineralization determined using the polarization microscopy was calculated to be p = -0.994. This in vitro experiment highlights the potential benefit of using FOBSS to detect demineralization and remineralization of enamel. PMID:20142887
NASA Astrophysics Data System (ADS)
Kotthaus, Simone; O'Connor, Ewan; Münkel, Christoph; Charlton-Perez, Cristina; Haeffelin, Martial; Gabey, Andrew M.; Grimmond, C. Sue B.
2016-08-01
Ceilometer lidars are used for cloud base height detection, to probe aerosol layers in the atmosphere (e.g. detection of elevated layers of Saharan dust or volcanic ash), and to examine boundary layer dynamics. Sensor optics and acquisition algorithms can strongly influence the observed attenuated backscatter profiles; therefore, physical interpretation of the profiles requires careful application of corrections. This study addresses the widely deployed Vaisala CL31 ceilometer. Attenuated backscatter profiles are studied to evaluate the impact of both the hardware generation and firmware version. In response to this work and discussion within the CL31/TOPROF user community (TOPROF, European COST Action aiming to harmonise ground-based remote sensing networks across Europe), Vaisala released new firmware (versions 1.72 and 2.03) for the CL31 sensors. These firmware versions are tested against previous versions, showing that several artificial features introduced by the data processing have been removed. Hence, it is recommended to use this recent firmware for analysing attenuated backscatter profiles. To allow for consistent processing of historic data, correction procedures have been developed that account for artefacts detected in data collected with older firmware. Furthermore, a procedure is proposed to determine and account for the instrument-related background signal from electronic and optical components. This is necessary for using attenuated backscatter observations from any CL31 ceilometer. Recommendations are made for the processing of attenuated backscatter observed with Vaisala CL31 sensors, including the estimation of noise which is not provided in the standard CL31 output. After taking these aspects into account, attenuated backscatter profiles from Vaisala CL31 ceilometers are considered capable of providing valuable information for a range of applications including atmospheric boundary layer studies, detection of elevated aerosol layers, and model verification.
Intercomparison of Atmospheric Correction Algorithms Applied to HICO Imagery
2011-03-02
photons and shadows a region. The water-leaving radiance from the shadowed region, LwsJu (A) that reaches the sensor results from only skylight ...solar and skylight photons. In addition to the water-leaving radiance, radiance recorded at the sensor also includes path radiance due to molecular (or...one part caused by the backscattering of the diffuse skylight and the other part by backscattering of the direct solar beam. For the sunny and
Probabilistic Verification of Multi-Robot Missions in Uncertain Environments
2015-11-01
has been used to measure the environment, including any dynamic obstacles. However, no matter how the model originates, this approach is based on...modeled as bivariate Gaussian distributions and estimated by calibration measurements . The Robot process model is described in prior work [13...sn〉 (pR,pE)(obR) = In〈pR〉〈p〉 ; In〈pE〉〈e〉 ; ( Gtr〈 d(p,e), sr〉〈p1〉 ; Out〈obR,p1〉 | Lte 〈 d(p,e), sr〉〈p2〉 ; Out〈obR, sn+p2 〉 ) ; Sensors
Adaptive focus for deep tissue using diffuse backscatter
NASA Astrophysics Data System (ADS)
Kress, Jeremy; Pourrezaei, Kambiz
2014-02-01
A system integrating high density diffuse optical imaging with adaptive optics using MEMS for deep tissue interaction is presented. In this system, a laser source is scanned over a high density fiber bundle using Digital Micromirror Device (DMD) and channeled to a tissue phantom. Backscatter is then collected from the tissue phantom by a high density fiber array of different fiber type and channeled to CMOS sensor for image acquisition. Intensity focus is directly verified using a second CMOS sensor which measures intensity transmitted though the tissue phantom. A set of training patterns are displayed on the DMD and backscatter is numerically fit to the transmission intensity. After the training patterns are displayed, adaptive focus is performed using only the backscatter and fitting functions. Additionally, tissue reconstruction and prediction of interference focusing by photoacoustic and optical tomographic methods is discussed. Finally, potential NIR applications such as in-vivo adaptive neural photostimulation and cancer targeting are discussed.
European Science Notes Information Bulletin. Reports on Current European and Middle Eastern Science
1993-04-01
beamwidth of 2 degrees . Hie had interesting data wideband backscatter in the 2-50 kHz band and on profiles, including hydrothermal plumes ob- forward...civil aviation. Academician P. Naza- gravity in casting Al-Pb alloys. This process renko is rector of the institut’. and Professor N . produces a...pro- east -west sections across 36’ N from Gibraltar to grams such as WOCE (World Ocean Circulation Bermuda and northwest across the Gulf Stream
Lu, Fei; Wang, Haixing; Guo, Yanjie; Tan, Qiulin; Zhang, Wendong; Xiong, Jijun
2018-01-16
A wireless and passive temperature sensor operating up to 800 °C is proposed. The sensor is based on microwave backscatter RFID (radio frequency identification) technology. A thin-film planar structure and simple working principle make the sensor easy to operate under high temperature. In this paper, the proposed high temperature sensor was designed, fabricated, and characterized. Here the 99% alumina ceramic with a dimension of 40 mm × 40 mm × 1 mm was prepared in micromechanics for fabrication of the sensor substrate. The metallization of the Au slot patch was realized in magnetron sputtering with a slot width of 2 mm and a slot length of 32 mm. The measured resonant frequency of the sensor at 25 °C is 2.31 GHz. It was concluded that the resonant frequency decreases with the increase in the temperature in range of 25-800 °C. It was shown that the average sensor sensitivity is 101.94 kHz/°C.
CO2 lidar backscatter profiles over Hawaii during fall 1988
NASA Technical Reports Server (NTRS)
Post, Madison J.; Cupp, Richard E.
1992-01-01
Aerosol and cloud backscatter data, obtained over a 24-day period in fall 1988 with the National Oceanic and Atmospheric Administration's Doppler lidar at 10.59-micron wavelength, are analyzed by using a new technique to lessen biases that are due to dropouts. Typical backscatter cross sections were significantly lower than those routinely observed over the continental United States, although episodic backscatter enhancements caused by cirrus and mineral dust also occurred. Implications of these data on the proposed Laser Atmospheric Wind Sounder wind profiling satellite sensor are discussed.
OBS development for long term observation in the Marmara Sea, NW Turkey
NASA Astrophysics Data System (ADS)
Takahashi, Narumi; Shimizu, Satoshi; Maekawa, Takuya; Kalafat, Dogan; Pinar, Ali; Citak, Seckin; Kaneda, Yoshiyuki
2015-04-01
We have carried out a collaboration study between Japan and Turkey since 2013, which is one of SATREPS projects, "Earthquake and Tsunami Disaster Mitigation in The Marmara Region and Disaster Education in Turkey". The main objective of this project is to reduce risk brought by earthquakes and tsunamis. In particular, the North Anatolian Fault system runs through the Marmara sea and it is expected that the seismic gap exists there according to past seismic studies. The details of seismicity distribution in the Marmara Sea is, however, still insufficient to construct fault model along the active faults. Therefore, we prepare ten ocean bottom seismographs (OBSs) to realize long term observation. We aim to identify size and depth of seismogenic zones using micro seismicity. In addition, we need to cover relative broad area from off-shore Istanbul city to the western end of the Marmara Sea. To clear these conditions, OBS specifications we need are high dynamic range and low instrument noise to observe micro seismicity, low electrical consumption to realize long term observation of over one year, high cost performance to cover the broad area for OBS installation, low cost implementation, and good operability to treat by relatively small number of persons. All items, which are three components velocity sensor, batteries, a recorder, a GPS receiver, a transponder and its transducer to control OBS retrieval, a flasher and a beacon, are installed in the 17 inches glass sphere. The natural frequency of the velocity sensor is 4.5 Hz and the frequency range of our OBS is from 4.5 Hz to 250 Hz. Data sampling is selectable among 100 Hz, 250 Hz and 500 Hz. Because our OBS is deployed by free fall, accuracy of the OBS clock is essentially one of important factors, and it is less than 0.1 ppm. And the resolution of A/D conversion performed on the recorder is 24 bit and we keep the dynamic range of over 135 dB. These data is stored on a semiconductor memory and the capacity is over 300 days with 100 Hz sampling observation. We adopted noncontact charge using lithium ion batteries to reduce implementation cost. And we can charge batteries and transfer stored data without opening glass sphere. All parameters of each OBS are controlled using wireless LAN. In this presentation, we introduce details of OBS development for Japan-Turkey project.
Prediction of Backscatter and Emissivity of Snow at Millimeter Wavelengths.
1980-01-01
LIST OF FIGURES Figure Title Page C-I General Area Map 204 C-2 Location of Alpena National Weather 205 Service Station C-3 Photographs of Site E 206 C-4...February 5, 6, 7, 1979 were ob- tained from National Weather Service, Alpena Station and included in Tables C-9 through C-12. Figure C-2 shows the...relative position * 1 of the sites and the Alpena National Weather Service Station. 203 I 0 1 s LAKE HURON ROGERS CITY 20 0 5 10 MILES 0 C HEBOYGAN NORTH
Modeling and characterization of different channels based on human body communication.
Jingzhen Li; Zedong Nie; Yuhang Liu; Lei Wang
2017-07-01
Human body communication (HBC), which uses the human body as a transmission medium for electrical signals, provides a prospective communication solution for body sensor networks (BSNs). In this paper, an inhomogeneous model which includes the tissue layers of skin, fat, and muscle is proposed to study the propagation characteristics of different HBC channels. Specifically, the HBC channels, namely, the on-body to on-body (OB-OB)channel, on-body to in-body (OB-IB) channel, in-body to on-body (IB-OB) channel, and in-body to in-body (IB-IB)channel, are studied over different frequencies (from 1MHz to 100MHz) through numerical simulations with finite-difference time-domain (FDTD) method. The results show that the gain of OB-IB channel and IB-OB channel is almost the same. The gain of IB-IB channel is greater than other channels in the frequency range 1MHz to 70MHz. In addition, the gain of all channels is associated with the channel length and communication frequency. The simulations are verified by experimental measurements in a porcine tissue sample. The results show that the simulations are in agreement with the measurements.
Curtis, Jennifer A.; Flint, Lorraine E.; Alpers, Charles N.; Wright, Scott A.; Snyder, Noah P.
2006-01-01
Sediment transport in the upper Yuba River watershed, California, was evaluated from October 2001 through September 2003. This report presents results of a three-year study by the U.S. Geological Survey, in cooperation with the California Ecosystem Restoration Program of the California Bay-Delta Authority and the California Resources Agency. Streamflow and suspended-sediment concentration (SSC) samples were collected at four gaging stations; however, this report focuses on sediment transport at the Middle Yuba River (11410000) and the South Yuba River (11417500) gaging stations. Seasonal suspended-sediment rating curves were developed using a group-average method and non-linear least-squares regression. Bed-load transport relations were used to develop bed-load rating curves, and bed-load measurements were collected to assess the accuracy of these curves. Annual suspended-sediment loads estimated using seasonal SSC rating curves were compared with previously published annual loads estimated using the Graphical Constituent Loading Analysis System (GCLAS). The percent difference ranged from -85 percent to +54 percent and averaged -7.5 percent. During water year 2003 optical backscatter sensors (OBS) were installed to assess event-based suspended-sediment transport. Event-based suspended-sediment loads calculated using seasonal SSC rating curves were compared with loads calculated using calibrated OBS output. The percent difference ranged from +50 percent to -369 percent and averaged -79 percent. The estimated average annual sediment yield at the Middle Yuba River (11410000) gage (5 tons/mi2) was significantly lower than that estimated at the South Yuba River (11417500) gage (14 tons/mi2). In both rivers, bed load represented 1 percent or less of the total annual load throughout the project period. Suspended sediment at the Middle Yuba River (11410000) and South Yuba River (11417500) gages was typically greater than 85 percent silt and clay during water year 2003, and sand concentrations at the South Yuba River (11417500) gage were typically higher than those at the Middle Yuba River (11410000) gage for a given streamflow throughout the three year project period. Factors contributing to differences in sediment loads and grain-size distributions at the Middle Yuba River (11410000) and South Yuba River (11417500) gages include contributing drainage area, flow diversions, and deposition of bed-material-sized sediment in reservoirs upstream of the Middle Yuba River (11410000) gage. Owing to its larger drainage area, higher flows, and absence of man-made structures that restrict sediment movement in the lower basin, the South Yuba River transports a greater and coarser sediment load.
Velocity and sediment surge: What do we see at times of very shallow water on intertidal mudflats?
NASA Astrophysics Data System (ADS)
Zhang, Qian; Gong, Zheng; Zhang, Changkuan; Townend, Ian; Jin, Chuang; Li, Huan
2016-02-01
A self-designed "bottom boundary layer hydrodynamic and suspended sediment concentration (SSC) measuring system" was built to observe the hydrodynamic and the SSC processes over the intertidal mudflats at the middle part of the Jiangsu coast during August 8-10, 2013. Velocity profiles within 10 cm of the mudflat surface were obtained with a vertical resolution as fine as 1 mm. An ADCP was used to extend the profile over the full water depth with a resolution of 10 cm and the vertical SSC profile was measured at intervals using Optical Backscatter Sensors (OBS). At the same time, water levels and wave conditions were measured with a Tide and Wave Recorder. Measured data suggested that the vertical structure of velocity profiles within 10 cm above the bed maintains a logarithmic distribution during the whole tidal cycle except the slack-water periods. Shallow flows during both the early-flood period and the later-ebb period are characterized by a relatively large vertical velocity gradient and a "surge" feature. We conclude that the very shallow water stages are transient and may not contribute much to the whole water and sediment transport, while they can play a significant role in the formation and evolution of micro-topographies on tidal flats.
Laboratory evaluation of the Sequoia Scientific LISST-ABS acoustic backscatter sediment sensor
Snazelle, Teri T.
2017-12-18
Sequoia Scientific’s LISST-ABS is an acoustic backscatter sensor designed to measure suspended-sediment concentration at a point source. Three LISST-ABS were evaluated at the U.S. Geological Survey (USGS) Hydrologic Instrumentation Facility (HIF). Serial numbers 6010, 6039, and 6058 were assessed for accuracy in solutions with varying particle-size distributions and for the effect of temperature on sensor accuracy. Certified sediment samples composed of different ranges of particle size were purchased from Powder Technology Inc. These sediment samples were 30–80-micron (µm) Arizona Test Dust; less than 22-µm ISO 12103-1, A1 Ultrafine Test Dust; and 149-µm MIL-STD 810E Silica Dust. The sensor was able to accurately measure suspended-sediment concentration when calibrated with sediment of the same particle-size distribution as the measured. Overall testing demonstrated that sensors calibrated with finer sized sediments overdetect sediment concentrations with coarser sized sediments, and sensors calibrated with coarser sized sediments do not detect increases in sediment concentrations from small and fine sediments. These test results are not unexpected for an acoustic-backscatter device and stress the need for using accurate site-specific particle-size distributions during sensor calibration. When calibrated for ultrafine dust with a less than 22-µm particle size (silt) and with the Arizona Test Dust with a 30–80-µm range, the data from sensor 6039 were biased high when fractions of the coarser (149-µm) Silica Dust were added. Data from sensor 6058 showed similar results with an elevated response to coarser material when calibrated with a finer particle-size distribution and a lack of detection when subjected to finer particle-size sediment. Sensor 6010 was also tested for the effect of dissimilar particle size during the calibration and showed little effect. Subsequent testing revealed problems with this sensor, including an inadequate temperature compensation, making this data questionable. The sensor was replaced by Sequoia Scientific with serial number 6039. Results from the extended temperature testing showed proper temperature compensation for sensor 6039, and results from the dissimilar calibration/testing particle-size distribution closely corroborated the results from sensor 6058.
Distributed Fiber-Optic Sensors for Vibration Detection
Liu, Xin; Jin, Baoquan; Bai, Qing; Wang, Yu; Wang, Dong; Wang, Yuncai
2016-01-01
Distributed fiber-optic vibration sensors receive extensive investigation and play a significant role in the sensor panorama. Optical parameters such as light intensity, phase, polarization state, or light frequency will change when external vibration is applied on the sensing fiber. In this paper, various technologies of distributed fiber-optic vibration sensing are reviewed, from interferometric sensing technology, such as Sagnac, Mach–Zehnder, and Michelson, to backscattering-based sensing technology, such as phase-sensitive optical time domain reflectometer, polarization-optical time domain reflectometer, optical frequency domain reflectometer, as well as some combinations of interferometric and backscattering-based techniques. Their operation principles are presented and recent research efforts are also included. Finally, the applications of distributed fiber-optic vibration sensors are summarized, which mainly include structural health monitoring and perimeter security, etc. Overall, distributed fiber-optic vibration sensors possess the advantages of large-scale monitoring, good concealment, excellent flexibility, and immunity to electromagnetic interference, and thus show considerable potential for a variety of practical applications. PMID:27472334
Distributed Fiber-Optic Sensors for Vibration Detection.
Liu, Xin; Jin, Baoquan; Bai, Qing; Wang, Yu; Wang, Dong; Wang, Yuncai
2016-07-26
Distributed fiber-optic vibration sensors receive extensive investigation and play a significant role in the sensor panorama. Optical parameters such as light intensity, phase, polarization state, or light frequency will change when external vibration is applied on the sensing fiber. In this paper, various technologies of distributed fiber-optic vibration sensing are reviewed, from interferometric sensing technology, such as Sagnac, Mach-Zehnder, and Michelson, to backscattering-based sensing technology, such as phase-sensitive optical time domain reflectometer, polarization-optical time domain reflectometer, optical frequency domain reflectometer, as well as some combinations of interferometric and backscattering-based techniques. Their operation principles are presented and recent research efforts are also included. Finally, the applications of distributed fiber-optic vibration sensors are summarized, which mainly include structural health monitoring and perimeter security, etc. Overall, distributed fiber-optic vibration sensors possess the advantages of large-scale monitoring, good concealment, excellent flexibility, and immunity to electromagnetic interference, and thus show considerable potential for a variety of practical applications.
Functional imaging of cortical feedback projections to the olfactory bulb
Rothermel, Markus; Wachowiak, Matt
2014-01-01
Processing of sensory information is substantially shaped by centrifugal, or feedback, projections from higher cortical areas, yet the functional properties of these projections are poorly characterized. Here, we used genetically-encoded calcium sensors (GCaMPs) to functionally image activation of centrifugal projections targeting the olfactory bulb (OB). The OB receives massive centrifugal input from cortical areas but there has been as yet no characterization of their activity in vivo. We focused on projections to the OB from the anterior olfactory nucleus (AON), a major source of cortical feedback to the OB. We expressed GCaMP selectively in AON projection neurons using a mouse line expressing Cre recombinase (Cre) in these neurons and Cre-dependent viral vectors injected into AON, allowing us to image GCaMP fluorescence signals from their axon terminals in the OB. Electrical stimulation of AON evoked large fluorescence signals that could be imaged from the dorsal OB surface in vivo. Surprisingly, odorants also evoked large signals that were transient and coupled to odorant inhalation both in the anesthetized and awake mouse, suggesting that feedback from AON to the OB is rapid and robust across different brain states. The strength of AON feedback signals increased during wakefulness, suggesting a state-dependent modulation of cortical feedback to the OB. Two-photon GCaMP imaging revealed that different odorants activated different subsets of centrifugal AON axons and could elicit both excitation and suppression in different axons, indicating a surprising richness in the representation of odor information by cortical feedback to the OB. Finally, we found that activating neuromodulatory centers such as basal forebrain drove AON inputs to the OB independent of odorant stimulation. Our results point to the AON as a multifunctional cortical area that provides ongoing feedback to the OB and also serves as a descending relay for other neuromodulatory systems. PMID:25071454
NASA Astrophysics Data System (ADS)
Yamashita, Y.; Shinohara, M.; Yamada, T.; Shiobara, H.
2017-12-01
It is important to understand coupling between plates in a subduction zone for studies of earthquake generation. Recently low frequency tremor and very low frequency earthquake (VLFE) were discovered in plate boundary near a trench. These events (slow earthquakes) in shallow plate boundary should be related to slow slip on a plate boundary. For observation of slow earthquakes, Broad Band Ocean Bottom Seismometer (BBOBS) is useful, however a number of BBOBSs are limited due to cost. On the other hand, a number of Long-term OBSs (LT-OBSs) with recording period of one year are available. However, the LT-OBS has seismometer with a natural period of 1 second. Therefore frequency band of observation is slightly narrow for slow earthquakes. Therefore we developed a compact long-term broad-band OBS by replacement of the seismic sensor of the LT-OBSs to broadband seismometer.We adopted seismic sensor with natural period of 20 seconds (Trillium Compact Broadband Seismometer, Nanometrics). Because tilt of OBS on seafloor can not be controlled due to free-fall, leveling system for seismic sensor is necessary. The broadband seismic senor has cylinder shape with diameter of 90 mm and height of 100 mm, and the developed levelling system can mount the seismic sensor with no modification of shape. The levelling system has diameter of 160 mm and height of 110 mm, which is the same size as existing levelling system of the LT-OBS. The levelling system has two horizontal axes and each axis is driven by motor. Leveling can be performed up to 20 degrees by using micro-processor (Arduino). Resolution of levelling is less than one degree. The system immediately starts leveling by the power-on of controller. After levelling, the the seismic senor is powered and the controller records angles of levelling to SD RAM. Then the controller is shut down to consume no power. Compact long-term broadband ocean bottom seismometer is useful for observation of slow earthquakes on seafloor. In addition, seafloor observations of teleseismic events and deep earthquakes to estimate seismic structure of deep regions and observations of submarine volcanoes are expected.
Correlation studies of passive and active microwave data in the marginal ice zone
NASA Technical Reports Server (NTRS)
Comiso, J. C.
1991-01-01
The microwave radiative and backscatter characteristics of sea ice in an Arctic marginal ice zone have been studied using near-simultaneous passive and active synthetic aperture radar microwave data. Intermediate-resolution multichannel passive microwave data were registered and analyzed. Passive and active microwave data generally complement each other as the two sensors are especially sensitive to different physical properties of the sea ice. In the inner pack, undeformed first-year ice is observed to have low backscatter values but high brightness temperatures while multiyear ice has generally high backscatter values and low brightness temperatures. However, in the marginal ice zone, the signature and backscatter for multiyear ice are considerably different and closer to those of first-year ice. Some floes identified by photography as snow-covered thick ice have backscatter similar to that of new ice or open water while brash ice has backscatter similar to or higher than that of ridged ice.
Towards Snowpack Characterization using C-band Synthetic Aperture Radar (SAR)
NASA Astrophysics Data System (ADS)
Park, J.; Forman, B. A.
2017-12-01
Sentinel 1A and 1B, operated by the European Space Agency (ESA), carries a C-band synthetic aperture radar (SAR) sensor that can be used to monitor terrestrial snow properties. This study explores the relationship between terrestrial snow-covered area, snow depth, and snow water equivalent with Sentinel 1 backscatter observations in order to better characterize snow mass. Ground-based observations collected by the National Oceanic and Atmospheric Administration - Cooperative Remote Sensing Science and Technology Center (NOAA-CREST) in Caribou, Maine in the United States are also used in the comparative analysis. Sentinel 1 Ground Range Detected (GRD) imagery with Interferometric Wide swath (IW) were preprocessed through a series of steps accounting for thermal noise, sensor orbit, radiometric calibration, speckle filtering, and terrain correction using ESA's Sentinel Application Platform (SNAP) software package, which is an open-source module written in Python. Comparisons of dual-polarized backscatter coefficients (i.e., σVV and σVH) with in-situ measurements of snow depth and SWE suggest that cross-polarized backscatter observations exhibit a modest correlation between both snow depth and SWE. In the case of the snow-covered area, a multi-temporal change detection method was used. Results using Sentinel 1 yield similar spatial patterns as when using hyperspectral observations collected by the MODerate Resolution Imaging Spectroradiometer (MODIS). These preliminary results suggest the potential application of Sentinel 1A/1B backscatter coefficients towards improved discrimination of snow cover, snow depth, and SWE. One goal of this research is to eventually merge C-band SAR backscatter observations with other snow information (e.g., passive microwave brightness temperatures) as part of a multi-sensor snow assimilation framework.
Kwoun, Oh-Ig; Lu, Z.
2009-01-01
Using multi-temporal European Remote-sensing Satellites (ERS-1/-2) and Canadian Radar Satellite (RADARSAT-1) synthetic aperture radar (SAR) data over the Louisiana coastal zone, we characterize seasonal variations of radar backscat-tering according to vegetation type. Our main findings are as follows. First, ERS-1/-2 and RADARSAT-1 require careful radiometric calibration to perform multi-temporal backscattering analysis for wetland mapping. We use SAR backscattering signals from cities for the relative calibration. Second, using seasonally averaged backscattering coefficients from ERS-1/-2 and RADARSAT-1, we can differentiate most forests (bottomland and swamp forests) and marshes (freshwater, intermediate, brackish, and saline marshes) in coastal wetlands. The student t-test results support the usefulness of season-averaged backscatter data for classification. Third, combining SAR backscattering coefficients and an optical-sensor-based normalized difference vegetation index can provide further insight into vegetation type and enhance the separation between forests and marshes. Our study demonstrates that SAR can provide necessary information to characterize coastal wetlands and monitor their changes.
Characterization of Global Near-Nadir Backscatter for Remote Sensing Radar Design
NASA Technical Reports Server (NTRS)
Spencer, Michael W.; Long, David G.
2000-01-01
In order to evaluate side-lobe contamination from the near-nadir region for Ku-Band radars, a statistical characterization of global near-nadir backscatter is constructed. This characterization is performed for a variety of surface types using data from TRMM, Seasat, and Topex. An assessment of the relative calibration accuracy of these sensors is also presented.
Characterization of Global Near-Nadir Backscatter for Remote Sensing Radar Design
NASA Technical Reports Server (NTRS)
Spencer, Michael W.; Long, David G.
2000-01-01
In order to evaluate side-lobe contamination from the near-nadir region for Ku-Band radars, a statistical characterization of global near-nadir backscatter is constructed. This characterization is performed for a variety of surface types using data from TRMM, Seasat, and Topex. An assessment of the relative calibration accuracy of them sensors is also presented.
On-line carbon balance of yeast fermentations using miniaturized optical sensors.
Beuermann, Thomas; Egly, Dominik; Geoerg, Daniel; Klug, Kerris Isolde; Storhas, Winfried; Methner, Frank-Juergen
2012-03-01
Monitoring of microbiological processes using optical sensors and spectrometers has gained in importance over the past few years due to its advantage in enabling non-invasive on-line analysis. Near-infrared (NIR) and mid-infrared (MIR) spectrometer set-ups in combination with multivariate calibrations have already been successfully employed for the simultaneous determination of different metabolites in microbiological processes. Photometric sensors, in addition to their low price compared to spectrometer set-ups, have the advantage of being compact and are easy to calibrate and operate. In this work, the detection of ethanol and CO(2) in the exhaust gas during aerobic yeast fermentation was performed by two photometric gas analyzers, and dry yeast biomass was monitored using a fiber optic backscatter set-up. The optical sensors could be easily fitted to the bioreactor and exhibited high robustness during measuring. The ethanol content of the fermentation broth was monitored on-line by measuring the ethanol concentration in the fermentation exhaust and applying a conversion factor. The vapor/liquid equilibrium and the associated conversion factor strongly depend on the process parameter temperature but not on aeration and stirring rate. Dry yeast biomass was determined in-line by a backscattering signal applying a linear calibration. An on-line balance with a recovery rate of 95-97% for carbon was achieved with the use of three optical sensors (two infrared gas analyzers and one fiber optic backscatter set-up). Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
NASA Astrophysics Data System (ADS)
Lynch, James F.; Irish, James D.; Sherwood, Christopher R.; Agrawal, Yogesh C.
1994-08-01
During the winter of 1990-1991 an Acoustic BackScatter System (ABSS), five Optical Backscatterance Sensors (OBSs) and a Laser In Situ Settling Tube (LISST) were deployed in 90 m of water off the California coast for 3 months as part of the Sediment Transport Events on Shelves and Slopes (STRESS) experiment. By looking at sediment transport events with both optical (OBS) and acoustic (ABSS) sensors, one obtains information about the size of the particles transported as well as their concentration. Specifically, we employ two different methods of estimating "average particle size". First, we use vertical scattering intensity profile slopes (acoustical and optical) to infer average particle size using a Rouse profile model of the boundary layer and a Stokes law fall velocity assumption. Secondly, we use a combination of optics and acoustics to form a multifrequency (two frequency) inverse for the average particle size. These results are compared to independent observations from the LISST instrument, which measures the particle size spectrum in situ using laser diffraction techniques. Rouse profile based inversions for particle size are found to be in good agreement with the LISST results except during periods of transport event initiation, when the Rouse profile is not expected to be valid. The two frequency inverse, which is boundary layer model independent, worked reasonably during all periods, with average particle sizes correlating well with the LISST estimates. In order to further corroborate the particle size inverses from the acoustical and optical instruments, we also examined size spectra obtained from in situ sediment grab samples and water column samples (suspended sediments), as well as laboratory tank experiments using STRESS sediments. Again, good agreement is noted. The laboratory tank experiment also allowed us to study the acoustical and optical scattering law characteristics of the STRESS sediments. It is seen that, for optics, using the cross sectional area of an equivalent sphere is a very good first approximation whereas for acoustics, which is most sensitive in the region ka ˜ 1, the particle volume itself is best sensed. In concluding, we briefly interpret the history of some STRESS transport events in light of the size distribution and other information available. For one of the events "anomalous" suspended particle size distributions are noted, i.e. larger particles are seen suspended before finer ones. Speculative hypotheses for why this signature is observed are presented.
NASA Astrophysics Data System (ADS)
Hello, Y.; Yegikyan, M.; Charvis, P.; Verfaillie, R.; Philippe, O.
2015-12-01
There are several attempts to monitor real time seismic activity, using regional scale wired nodes, such as Neptune in Canada and in the U.S, Antares in France or DONET in Japan.On another hand there are also initiatives in deploying repeatedly OBS array like during the amphibious Cascadia Initiative (four 1-year deployments), the Japanese Pacific Array (broadband OBSs "ocean-bottom broadband dispersion survey" with 2-years autonomy), the Obsismer program in the French Lesser Antilles (eight 6-months deployments) and the Osisec program in Ecuador (four 6-months deployments). These OBSs are autonomous, they are self-recovered or recovered using an ROV. These systems are costly including ship time, and require to recover the OBS before to start working on data.Among the most recent alternative we developed a 3-years autonomy OBS equipped with a Nanometrics Trillium 120 s, a triaxial accelerometer, a differential, an absolute pressure gauge, and a hydrophone. MUG-OBS is a free falling instrument rated down to 6000 m. The installation of the sensor is monitored by acoustic commands from the surface and a health bulletin with data checking is recovered by acoustic during the installation. The major innovation is that it is possible to recover the data any time on demand (regularly every 6-months or after a seismic crisis) utilizing one of the 6 data-shuttles released from the surface by acoustic command using a one day fast cruise boat of opportunity. Since sensors stayed at the same location for 3 years (when an OBS is redeployed on the same site, it will not land in the same place), it is a perfect tool to monitor slow seismic events, background seismic activity and aftershock distribution. Clock, drift measurement and GPS localization is automatic when the shuttle reaches the surface. A new version is being developed; for remote areas, shuttles released automatically and a seismic events bulletin is transmitted. Selected data can be recovered by two- way Iridium satellite communication. After a period of 3 years the main station is self-recovered by acoustic.
Rainfall and River Currents Retrieved from Microwave Backscatter
Plant, W.J.; Keller, W.C.; Hayes, K.; Nystuen, J.; Spicer, K.
2003-01-01
The use of CW microwave sensors in yielding information on both river surface velocities and rain rates was discussed. Eight CW microwave sensors were installed at Cowlitz river in Western Washington State in the US. The sensors measured the river surface velocity via Doppler shifts at eight locations across the river. Comparison of the surface velocities derived from the sensors mounted on the bridge with those measured by current meters and acoustic sensors demonstrated good agreement.
NASA Astrophysics Data System (ADS)
Nechad, Bouchra; Alvera-Azcaràte, Aida; Ruddick, Kevin; Greenwood, Naomi
2011-08-01
In situ measurements of total suspended matter (TSM) over the period 2003-2006, collected with two autonomous platforms from the Centre for Environment, Fisheries and Aquatic Sciences (Cefas) measuring the optical backscatter (OBS) in the southern North Sea, are used to assess the accuracy of TSM time series extracted from satellite data. Since there are gaps in the remote sensing (RS) data, due mainly to cloud cover, the Data Interpolating Empirical Orthogonal Functions (DINEOF) is used to fill in the TSM time series and build a continuous daily "recoloured" dataset. The RS datasets consist of TSM maps derived from MODIS imagery using the bio-optical model of Nechad et al. (Rem Sens Environ 114: 854-866, 2010). In this study, the DINEOF time series are compared to the in situ OBS measured in moderately to very turbid waters respectively in West Gabbard and Warp Anchorage, in the southern North Sea. The discrepancies between instantaneous RS, DINEOF-filled RS data and Cefas data are analysed in terms of TSM algorithm uncertainties, space-time variability and DINEOF reconstruction uncertainty.
Remote measurements of water pollution with a lidar polarimeter
NASA Technical Reports Server (NTRS)
Sheives, T. C.; Rouse, J. W., Jr.; Mayo, W. T., Jr.
1974-01-01
This paper examines a dual polarization laser backscatter system as a method for remote measurements of certain water quality parameters. Analytical models for describing the backscatter from turbid water and oil on turbid water are presented and compared with experimental data. Laser backscatter field measurements from natural waterways are presented and compared with simultaneous ground observations of the water quality parameters: turbidity, suspended solids, and transmittance. The results of this study show that the analytical models appear valid and that the sensor investigated is applicable to remote measurements of these water quality parameters and oil spills on water.-
Su, Shujing; Lu, Fei; Wu, Guozhu; Wu, Dezhi; Tan, Qiulin; Dong, Helei; Xiong, Jijun
2017-08-25
The highly sensitive pressure sensor presented in this paper aims at wireless passive sensing in a high temperature environment by using microwave backscattering technology. The structure of the re-entrant resonator was analyzed and optimized using theoretical calculation, software simulation, and its equivalent lump circuit model was first modified by us. Micro-machining and high-temperature co-fired ceramic (HTCC) process technologies were applied to fabricate the sensor, solving the common problem of cavity sealing during the air pressure loading test. In addition, to prevent the response signal from being immersed in the strong background clutter of the hermetic metal chamber, which makes its detection difficult, we proposed two key techniques to improve the signal to noise ratio: the suppression of strong background clutter and the detection of the weak backscattered signal of the sensor. The pressure sensor demonstrated in this paper works well for gas pressure loading between 40 and 120 kPa in a temperature range of 24 °C to 800 °C. The experimental results show that the sensor resonant frequency lies at 2.1065 GHz, with a maximum pressure sensitivity of 73.125 kHz/kPa.
Regional Glacier Mapping by Combination of Dense Optical and SAR Satellite Image Time-Series
NASA Astrophysics Data System (ADS)
Winsvold, S. H.; Kääb, A.; Andreassen, L. M.; Nuth, C.; Schellenberger, T.; van Pelt, W.
2016-12-01
Near-future dense time series from both SAR (Sentinel-1A and B) and optical satellite sensors (Landsat 8, Sentinel-2A and B) will promote new multisensory time series applications for glacier mapping. We assess such combinations of optical and SAR data among others by 1) using SAR data to supplement optical time series that suffer from heavy cloud cover (chronological gap-filling), 2) merging the two data types based on stack statistics (Std.dev, Mean, Max. etc.), or 3) better explaining glacier facies patterns in SAR data using optical satellite images. As one example, summer SAR backscatter time series have been largely unexplored and even neglected in many glaciological studies due to the high content of liquid melt water on the ice surface and its intrusion in the upper part of the snow and firn. This water content causes strong specular scattering and absorption of the radar signal, and little energy is scattered back to the SAR sensor. We find in many scenes of a Sentinel-1 time series a significant temporal backscatter difference between the glacier ice surface and the seasonal snow as it melts up glacier. Even though both surfaces have typically wet conditions, we suggest that the backscatter difference is due to different roughness lengths of the two surfaces. Higher backscatter is found on the ice surface in the ablation area compared to the firn/seasonal snow surface. We find and present also other backscatter patterns in the Sentinel-1 time series related to glacier facies and weather events. For the Ny Ålesund area, Svalbard we use Radarsat-2 time series to explore the glacier backscatter conditions in a > 5 year period, discussing distinct temporal signals from among others refreezing of the firn in late autumn, or temporal lakes. All these examples are analyzed using the above 3 methods. By this multi-temporal and multi-sensor approach we also explore and describe the possible connection between combined SAR/optical time series and surface mass balance.
NASA Astrophysics Data System (ADS)
Laiolo, Paola; Gabellani, Simone; Rudari, Roberto; Boni, Giorgio; Puca, Silvia
2013-04-01
Soil moisture plays a fundamental role in the partitioning of mass and energy fluxes between land surface and atmosphere, thereby influencing climate and weather, and it is important in determining the rainfall-runoff response of catchments; moreover, in hydrological modelling and flood forecasting, a correct definition of moisture conditions is a key factor for accurate predictions. Different sources of information for the estimation of the soil moisture state are currently available: satellite data, point measurements and model predictions. All are affected by intrinsic uncertainty. Among different satellite sensors that can be used for soil moisture estimation three major groups can be distinguished: passive microwave sensors (e.g., SSMI), active sensors (e.g. SAR, Scatterometers), and optical sensors (e.g. Spectroradiometers). The last two families, mainly because of their temporal and spatial resolution seem the most suitable for hydrological applications In this work soil moisture point measurements from 10 sensors in the Italian territory are compared of with the satellite products both from the HSAF project SM-OBS-2, derived from the ASCAT scatterometer, and from ACHAB, an operative energy balance model that assimilate LST data derived from MSG and furnishes daily an evaporative fraction index related to soil moisture content for all the Italian region. Distributed comparison of the ACHAB and SM-OBS-2 on the whole Italian territory are performed too.
In-situ Observations of Swash-zone Flow Velocities and Sediment Transport on a Steep Beach
NASA Astrophysics Data System (ADS)
Chardon-Maldonado, P.; Puleo, J. A.; Figlus, J.
2014-12-01
A 45 m scaffolding frame containing an array of instruments was installed at South Bethany Beach, Delaware, to obtain in-situ measurements in the swash zone. Six cross-shore stations were established to simultaneously measure near-bed velocity profiles, sediment concentration and water level fluctuations on a steep beach. Measurements of swash-zone hydrodynamics and morphological change were collected from February 12 to 25, 2014, following a large Nor'easter storm with surf zone significant wave height exceeding 5 m. Swash-zone flow velocities (u,v,w) were measured at each cross-shore location using a Nortek Vectrino profiling velocimeter that measured a 30 mm velocity profile at 1 mm vertical increments at 100 Hz. These velocity profiles were used to quantify the vertical flow structure over the foreshore and estimate hydrodynamic parameters such as bed shear stress and turbulent kinetic energy dissipation. Sediment concentrations were measured using optical backscatter sensors (OBS) to obtain spatio-temporal measurements during both uprush and backwash phases of the swash cycle. Cross-shore sediment transport rates at each station were estimated by taking the product of cross-shore velocity and sediment concentration. Foreshore elevations were sampled every low tide using a Leica GPS system with RTK capability. Cross-shore sediment transport rates and gradients derived from the velocities and bed shear stress estimates will be related to the observed morphological change.
Experimental assessment of an RFID-based crack sensor for steel structures
NASA Astrophysics Data System (ADS)
E Martínez-Castro, R.; Jang, S.; Nicholas, J.; Bansal, R.
2017-08-01
The use of welded steel cover plates had been a common design practice to increase beam section capacity in regions of high moment for decades. Many steel girder bridges with cover plates are still in service. Steel girder bridges are subject to cyclic loading, which can initiate crack formation at the toe of the weld and reduce beam capacity. Thus, timely detection of fatigue cracks is of utmost importance in steel girder bridge monitoring. To date, crack monitoring methods using in-house radio frequency identification (RFID)-based sensors have been developed to complement visual inspection and provide quantitative information of damage level. Offering similar properties at a reduced cost, commercial ultra-high frequency (UHF) passive RFID tags have been identified as a more financially viable option for pervasive crack monitoring using a dense array of sensors. This paper presents a study on damage sensitivity of low-cost commercial UHF RFID tags for crack detection and monitoring on metallic structures. Using backscatter power as a parameter for damage identification, a crack sensing system has been developed for single and multiple tag configurations for increased sensing pervasiveness. The effect on backscatter power of the existence and stage of crack propagation has been successfully characterized. For further automation of crack detection, a damage index based on the variation of backscatter power has also been established. The tested commercial RFID-based crack sensor contributes to the usage of this technology on steel girder bridges.
Distributed measurement of high electric current by means of polarimetric optical fiber sensor.
Palmieri, Luca; Sarchi, Davide; Galtarossa, Andrea
2015-05-04
A novel distributed optical fiber sensor for spatially resolved monitoring of high direct electric current is proposed and analyzed. The sensor exploits Faraday rotation and is based on the polarization analysis of the Rayleigh backscattered light. Preliminary laboratory tests, performed on a section of electric cable for currents up to 2.5 kA, have confirmed the viability of the method.
Aerial ultrasonic micro Doppler sonar detection range in outdoor environments.
Bradley, Marshall; Sabatier, James M
2012-03-01
Current research demonstrates that micro Doppler sonar has the capability to uniquely identify the presence of a moving human, making it an attractive component in surveillance systems for border security applications. Primary environmental factors that limit sonar performance are two-way spreading losses, ultrasonic absorption, and backscattered energy from the ground that appears at zero Doppler shift in the sonar signal processor. Spectral leakage from the backscatter component has a significant effect on sonar performance for slow moving targets. Sonar performance is shown to rapidly decay as the sensor is moved closer to the ground due to increasing surface backscatter levels. © 2012 Acoustical Society of America
NASA Technical Reports Server (NTRS)
Cutten, D. R.; Spinhirne, J. D.; Menzies, R. T.; Bowdle, D. A.; Srivastava, V.; Pueschel, R. F.; Clarke, A. D.; Rothermel, J.
1998-01-01
Aerosol backscatter coefficient data were examined from two nights near Japan and Hawaii undertaken during NASA's Global Backscatter Experiment (GLOBE) in May-June 1990. During each of these two nights the aircraft traversed different altitudes within a region of the atmosphere defined by the same set of latitude and longitude coordinates. This provided an ideal opportunity to allow flight level focused continuous wave (CW) lidar backscatter measured at 9.11-micron wavelength and modeled aerosol backscatter from two aerosol optical counters to be compared with pulsed lidar aerosol backscatter data at 1.06- and 9.25-micron wavelengths. The best agreement between all sensors was found in the altitude region below 7 km, where backscatter values were moderately high at all three wavelengths. Above this altitude the pulsed lidar backscatter data at 1.06- and 9.25-micron wavelengths were higher than the flight level data obtained from the CW lidar or derived from the optical counters, suggesting sample volume effects were responsible for this. Aerosol microphysics analysis of data near Japan revealed a strong sea-salt aerosol plume extending upward from the marine boundary layer. On the basis of sample volume differences, it was found that large particles were of different composition compared with the small particles for low backscatter conditions.
Wide-Area Persistent Energy-Efficient Maritime Sensing
2015-09-30
Matt Reynolds, Lefteris Kampianakis, and Andreas Pedrosse-Engel at UW designed and tested a Software Defined Radar testbed as well as an Arduino - based ...hardware based on a software-defined radio platform. 2) Development of a standalone Arduino - based backscatter node. 3) Analysis of the limits of the... Arduino - based node that can modulate radar backscatter with data received from a sensor using a low-power Arduino Nano processor. Figure 5 shows a
NASA Astrophysics Data System (ADS)
Pazos, Antonio; Davila, Jose Martin; Buforn, Elisa; Bezzeghoud, Mourad; Harnafi, Mimoun; Mattesini, Mauricio; Caldeira, Bento; Hanka, Winfried; El Moudnib, Lahcen; Strollo, Angelo; Roca, Antoni; Lopez de Mesa, Mireya; Dahm, Torsten; Cabieces, Roberto
2016-04-01
The Western Mediterranean (WM) seismic network started in 1996 as an initiative of the Royal Spanish Navy Observatory (ROA) and the Universidad Complutense de Madrid (UCM), with the collaboration of the GeoForschungsZentrum (GFZ) of Potsdam. A first broad band seismic station (SFUC) was installed close to Cádiz (South Spain). Since then, additional stations have been installed in the Ibero-Moghrebian region. In 2005, the "WM" code was assigned by the FDSN and new partners were jointed: Evora University (UEVO, Portugal), the Scientifique Institute of Rabat (ISRABAT, Morocco), and GFZ. Now days, the WM network is composed by 15 BB stations, all of them with Streckaisen STS-2 or STS-2.5 sensors, Quanterra or Earthdata digitizers and SeiscomP. Most them have co-installed a permanent geodetic GPS stations, and some them also have an accelerometer. There are 10 stations deployed in Spanish territory (5 in the Iberian peninsula, 1 in Balearic islands and 4 in North Africa Spanish places) with VSAT or Internet communications, 2 in Portugal (one of them without real time), and 3 in Morocco (2 VSAT and 1 ADSL). Additionally, 2 more stations (one in South Spain and one in Morocco) will be installed along this year. Additionally ROA has deployed a permanent real time VBB (CMG-3T: 360s) station at the Alboran Island. Due to the fact that part of the seismic activity is located at marine areas, and also because of the poor geographic azimuthal coverage at some zones provided by the land stations (specially in the SW of the San Vicente Cape area), ROA and UCM have acquired six broad band "LOBSTERN" OBS, manufactured by KUM (Kiel, Germany), conforming the OBS FOMAR pool. Three of them with CMG-40T sensor and the other with Trillium 120. These OBS were deployed along the Gibraltar strait since January to November 2014 to study the microseismicity in the Gibraltar strait area. In September 2015 FOMAR network has been deployed in SW of the San Vicente Cape for 8 months as a part of ALERTES-RIM project. In this work the current state and future plans of the WM network, the results of the Gibraltar strait campaign and San Vicente Cape OBS experiment are shown.
Ship Noise in the SW Indian Ocean Recorded by Ocean Bottom Seismic and Hydroacoustic Sensors
NASA Astrophysics Data System (ADS)
Barruol, G.; Dreo, R.; Fontaine, F. R.; Scholz, J. R.; Sigloch, K.
2016-12-01
In the frame of the RHUM-RUM project (Réunion Hotspot and Upper Mantle - Réunions Unterer Mantel, www.rhum-rum.net), a network of 57 ocean-bottom seismometers (OBS) has been installed on the ocean floor around La Réunion Island, but also on the neighbouring Southwest and Central Indian Ridges. The OBS were equipped by wide- and broad-band three-components seismic and hydroacoustic sensors. They were deployed in Nov. 2012, and depending on the configuration, they recorded for 8 to 13 months. Interestingly, part of the network was located beneath a NE-SW trending lane of very dense ship traffic connecting SE-Asia and the South-Atlantic region. By combining the vessel position - provided by AIS GPS data - and our geophysical data recorded on the ocean floor, we analyze the seismic and hydroacoustic ship signatures. From spectral analyzes, we show clear signals over the whole high-frequency range available from our instruments (between 1 and 50 Hz). The RHUM-RUM network covering latitude between 17 and 34° South, this allows to detect numerous vessels and to compare the noise characteristics (frequency content, polarization) of each vessel. We also investigate the possibility of using the polarization of the noise emitted by ships passing above an ocean-bottom seismometer, to help retrieving the orientation of the OBS horizontal components on the ocean floor in the geographic reference frame.
An investigation of the observability of ocean-surface parameters using GEOS-3 backscatter data
NASA Technical Reports Server (NTRS)
Miller, L. S.; Priester, R. W.
1978-01-01
The degree to which ocean surface roughness can be synoptically observed through use of the information extracted from the GEOS-3 backscattered waveform data was evaluated. Algorithms are given for use in estimating the radar sensed waveheight distribution or ocean-surface impulse response. Other factors discussed include comparisons between theoretical and experimental radar cross section values, sea state bias effects, spatial variability of significant waveheight data, and sensor-related considerations.
The Geostationary Trace gas and Aerosol Sensor Optimization (GeoTASO) airborne instrument is a test bed for upcoming air quality satellite instruments that will measure backscattered ultraviolet, visible and near-infrared light from geostationary orbit. GeoTASO flew on the NASA F...
Turbulence Characterization and Control
1975-07-01
backscatter experiment, measures the returns from density fluctuations over the lower 1000 ft. of the atmosphere. Microthermal sensors measure local... microthermal sensors mentioned above provide useful data of this type. In additiona an optical experiment capable of making quantitative...analysis of these data has been completed. During some of these experimental runs, microthermal and acoustic sounder data was also col- lected by
NASA Astrophysics Data System (ADS)
Renaud, Rémi; Bendahmane, Mounir; Chery, Romain; Martin, Claire; Gurden, Hirac; Pain, Frederic
2012-06-01
Wide field multispectral imaging of light backscattered by brain tissues provides maps of hemodynamics changes (total blood volume and oxygenation) following activation. This technique relies on the fit of the reflectance images obtain at two or more wavelengths using a modified Beer-Lambert law1,2. It has been successfully applied to study the activation of several sensory cortices in the anesthetized rodent using visible light1-5. We have carried out recently the first multispectral imaging in the olfactory bulb6 (OB) of anesthetized rats. However, the optimization of wavelengths choice has not been discussed in terms of cross talk and uniqueness of the estimated parameters (blood volume and saturation maps) although this point was shown to be crucial for similar studies in Diffuse Optical Imaging in humans7-10. We have studied theoretically and experimentally the optimal sets of wavelength for multispectral imaging of rodent brain activation in the visible. Sets of optimal wavelengths have been identified and validated in vivo for multispectral imaging of the OB of rats following odor stimulus. We studied the influence of the wavelengths sets on the magnitude and time courses of the oxy- and deoxyhemoglobin concentration variations as well as on the spatial extent of activated brain areas following stimulation. Beyond the estimation of hemodynamic parameters from multispectral reflectance data, we observed repeatedly and for all wavelengths a decrease of light reflectance. For wavelengths longer than 590 nm, these observations differ from those observed in the somatosensory and barrel cortex and question the basis of the reflectance changes during activation in the OB. To solve this issue, Monte Carlo simulations (MCS) have been carried out to assess the relative contribution of absorption, scattering and anisotropy changes to the intrinsic optical imaging signals in somatosensory cortex (SsC) and OB model.
Observations of the Variability of Floc Sizes on the Louisiana Shelf
NASA Astrophysics Data System (ADS)
Sahin, Cihan; Sheremet, Alexandru
2014-05-01
The general principles of floc formation under variable turbulent stresses and sediment availability are well known, but the details of the dynamics are still unclear. Flocculation of primary particles occurs when these particles get close enough to collide, and a significant number of these collisions result in adhesion. Particle concentration, the intensity and number of collisions (turbulent shear) control the size of the flocs. However, aggregation transitions into fragmentation if the intensity of collisions or turbulent shear exceeds a certain threshold. In this case, a limiting maximum size might exist (Berhane et al., 1997; Dyer and Manning, 1999; Uncles et al., 2010). This study investigates the relation between SSC (suspended sediment concentration), turbulent stresses, and floc size using the high-resolution observations of suspended sediment concentration, flow and acoustic backscatter made for 2 weeks in Spring 2008 on the muddy Atchafalaya Shelf. During the experiment, pressure, near-bed current velocities, and acoustic backscatter profiles were sampled using a downward-pointing 1500-kHz PC-ADP (Pulse-Coherent Acoustic Doppler Profiler, Sontek/YSI). In addition, a downward-pointing single frequency ABS (Acoustic Backscatter Sensor, 700-kHz, Marine Electronics, Isle of Guernsey) measured the intensity of acoustic return in the first meter above bed. Thus, acoustic backscatter profiles were observed by two different frequencies (700 kHz for the ABS and 1500 kHz for the PC-ADP). Direct SSC observations were provided by two OBS-3s at 15 and 40-cm above the bed, which sampled synchronously with the PC-ADP. Simultaneous profiles of SSC and the mean floc size at cm-scale vertical resolution were obtained using acoustic backscatter intensity at the different acoustic frequencies. For the calibration of the instruments, which involves estimation of the instruments system constants, the algorithm described in Sahin et al. (2013) was followed. The mean floc size profiles were obtained at each range bin and propagated along the backscattered profile using the implicit iterative approach, which is based on calculation of SSC and mean size iteratively until the values in the last two iterations converge (Sahin, under review). Estimated SSC profiles are in agreement with OBS point measurements with a correlation coefficient of 0.88 and 0.12 kg/m3 RMS error. The range of floc size estimates is consistent with the floc-size measurements in 2006 at the same site. Turbulent shear profiles were estimated using the current velocity profiles measured by the PC-ADP. As a first step, the friction velocities are estimated following Lacy et. al. (2005) by fitting the logarithmic profiles in a least-square sense to the current velocity profiles outside the wave boundary layer (Safak et al., 2013), which can then be used to approximate the dissipation of turbulent kinetic energy and the turbulent shear for each bin. The detailed investigation of the resulting SSC, floc-size and the turbulent shear rate profiles showed that low-to-mid values of shear rate promotes flocculation by increasing cohesive sediment particle collision. However, higher turbulent shear strongly affects large flocs, which have weaker internal bonds than those of the primary particles. These field observations support the findings observed in several previous experimental and numerical studies (Parker et al., 1972; Dyer, 1989). Acknowledgements This research was supported by the Office of Naval Research funding of contracts N00014-10-1-0363 and N00014-11-1-0269. References Berhane, I., Sternberg R.W., Kineke G.C., Milligan T.G., Kranck, K., 1997. The variability of suspended aggregates on the Amazon Continental Shelf. Continental Shelf Research 17, 267-285. Dyer, K. R., 1989. Sediment processes in estuaries: future research requirements. Journal of Geophysical Research 97, 14327-14339. Dyer, K. R., Manning A.J., 1999. Observation of size, settling velocity and effective density of flocs, and their fractal dimensions. J. Sea. Res., 41, 87-95. Hay, A.E. and Sheng J., 1992. Vertical Profiles of Suspended Sand Concentration and Size From Multifrequency Acoustic Backscatter. J. Geophys. Res., 97, C10, doi:10.1029/92JC01240. Lacy, J.R., Sherwood, C.R., Wilson, D.J., Chisholm, T.A., Gelfenbaum, G.R., 2005. Characteristics of suspended particles at an 11 hour anchor station in San Francisco Bay. Journal of Geophysical Research 110, C06014, http://dx.doi.org/10.1029/2003JC001814. Parker, D.S., Kaufman, and W.J., Jenkins, D., 1972. Floc breakup in turbulent processes. Journal of the Sanitary Engineering Division, ASCE , SA1, pp. 79-99. Safak, I., Allison M. A., Sheremet A., 2013. Floc variability under changing turbulent stresses and sediment availability on a wave energetic muddy shelf. Continental Shelf Research 53, 1-10, http://dx.doi.org/10.1016/j.csr.2012.11.015. Sahin, C., Safak I., Hsu T.-J., Sheremet A., 2013. Observations of suspended sediment stratification from acoustic backscatter in muddy environments. Marine Geology 336, 24-32, http://dx.doi.org/10.1016/j.margeo.2012.12.001. Sahin, C., under review. Investigation of the variability of floc sizes on the Louisiana Shelf using acoustic estimates of cohesive sediment properties. Uncles, R.J., Bale A.J., Stephens J.A., Frickers P.E., Harris, C., 2010. Observations of floc sizes in a muddy estuary. Estuarine, Coastal and Shelf Science 87, 186-196.
NASA Astrophysics Data System (ADS)
Kuznetsov, A. G.; Babin, Sergei A.; Shelemba, Ivan S.
2009-11-01
We demonstrate a Raman-based all-fibre temperature sensor utilising a pulsed erbium fibre laser. The sensor is made of a standard single-mode telecom fibre, SMF-28, and includes a number of directional couplers as band-pass filters. The temperature profile along a 7-km fibreoptic line is measured with an accuracy of 2oC and a spatial resolution of 10 m. In data processing, we take into account the difference in attenuation between the spectral components of the backscatter signal.
Ocean Bottom Seismometer Augmentation of the Philippine Sea Experiment (OBSAPS) Cruise Report
2011-09-01
single 77.5Hz M-sequence on six OBSAPS receivers: (from bottom to top) the vertical geophone on the North OBS ( blue ), the hydrophone module on the...wet end electronics (pressure sensor, hydrophone and octopus ) to the spare J15-3 S/N 14 and re-deployed the transducer and tow body assembly. We then...our wet end electronics (hydrophone, pressure sensor and octopus ) to the S/N 11 unit and re-deployed. The repaired J15-3 S/N 11 unit performed
Airborne lidar measurements of El Chichon stratospheric aerosols, October 1982 to November 1982
NASA Technical Reports Server (NTRS)
Mccormick, M. P.; Osborn, M. T.
1985-01-01
A coordinated flight mission to determine the spatial distribution and aerosol characteristics of the El Chichon produced stratospheric aerosol was flown in October to November 1982. The mission covered 46 deg N to 46 deg S and included rendezvous between balloon-, airplane-, and satellite-borne sensors. The lidar data from the flight mission are presented. Representative profiles of lidar backscatter ratio, plots of the integrated backscattering function versus latitude, and contours of backscatter mixing ratio versus altitude and latitude are given. In addition, tables containing numerical values of the backscatter ratio and backscattering functions versus altitude are supplied for each profile. The bulk of the material produced by the El Chichon eruptions of late March 10 to early April 1982 resided between latitudes from 5 to 7 deg S to 35 to 37 deg N and was concentrated above 21 km in a layer that peaked at 23 to 25 km. In this latitude region, peak scattering ratios at a wavelength of 0.6943 micron were approximately 24. The results of this mission are presented in a ready-to-use format for atmospheric and climatic studies.
OBSIP: Advancing Capabilities and Expanding the Ocean Bottom Seismology Community
NASA Astrophysics Data System (ADS)
Aderhold, K.; Evers, B.
2016-12-01
The Ocean Bottom Seismograph Instrument Pool (OBSIP) is a National Science Foundation sponsored instrument facility that provides ocean bottom seismometers (OBS) and technical support for research in the areas of marine geology, seismology, and geodynamics. OBSIP is comprised of an OBSIP Management Office (OMO) and three Institutional Instrument Contributors (IICs), who each contribute instruments and technical support to the pool. OBSIP operates both short period and broadband OBS instruments with a variety of capabilities to operate in shallow or deep water over both short and long term durations. Engineering developments at the IICs include capability for freshwater deployments, increased recording duration (15+ months), more efficient recovery systems, and sensor upgrades for a less heterogeneous fleet. OBSIP will provide instruments for three experiments in 2016 with deployments along a 1500 km transect in the South Atlantic, a large active-source experiment on the Chilean megathrust, and the very first seismometers ever deployed in Yellowstone Lake. OBSIP OMO strives to lower the barrier to working with OBS data by performing quality checks on data, investigating and responding to community questions, and providing data products like horizontal orientation calculations. This has resulted in a significant increase in new users to OBS data, especially for the open data sets from community seismic experiments. In 2015 the five-year Cascadia Initiative community seismic experiment concluded with over 250 OBS deployments and recoveries in an extensive grid off-shore Washington, Oregon, and California. The logistics of the Cascadia Initiative were challenging, but lessons were learned and efficiencies have been identified for implementation in future experiments. Large-scale community seismic experiments that cross the shoreline like the Cascadia Initiative and the Eastern North American Margin experiment have led to the proposal of even more ambitious endeavors like the Subduction Zone Observatory. OBSIP also is working to develop international collaboration and networking between OBS operators and researchers through special interest group meetings and the biannual OBS Symposium, to be held again in Fall 2017.
NASA Technical Reports Server (NTRS)
Soreide, David C.; Bogue, Rodney K.; Ehernberger, L. J.; Hannon, Stephen M.; Bowdle, David A.
2000-01-01
The purpose of the ACLAIM program is ultimately to establish the viability of light detection and ranging (lidar) as a forward-looking sensor for turbulence. The goals of this flight test are to: 1) demonstrate that the ACLAIM lidar system operates reliably in a flight test environment, 2) measure the performance of the lidar as a function of the aerosol backscatter coefficient (beta), 3) use the lidar system to measure atmospheric turbulence and compare these measurements to onboard gust measurements, and 4) make measurements of the aerosol backscatter coefficient, its probability distribution and spatial distribution. The scope of this paper is to briefly describe the ACLAIM system and present examples of ACLAIM operation in flight, including comparisons with independent measurements of wind gusts, gust-induced normal acceleration, and the derived eddy dissipation rate.
Biogeochemical sensor performance in the SOCCOM profiling float array
NASA Astrophysics Data System (ADS)
Johnson, Kenneth S.; Plant, Joshua N.; Coletti, Luke J.; Jannasch, Hans W.; Sakamoto, Carole M.; Riser, Stephen C.; Swift, Dana D.; Williams, Nancy L.; Boss, Emmanuel; Haëntjens, Nils; Talley, Lynne D.; Sarmiento, Jorge L.
2017-08-01
The Southern Ocean Carbon and Climate Observations and Modeling (SOCCOM) program has begun deploying a large array of biogeochemical sensors on profiling floats in the Southern Ocean. As of February 2016, 86 floats have been deployed. Here the focus is on 56 floats with quality-controlled and adjusted data that have been in the water at least 6 months. The floats carry oxygen, nitrate, pH, chlorophyll fluorescence, and optical backscatter sensors. The raw data generated by these sensors can suffer from inaccurate initial calibrations and from sensor drift over time. Procedures to correct the data are defined. The initial accuracy of the adjusted concentrations is assessed by comparing the corrected data to laboratory measurements made on samples collected by a hydrographic cast with a rosette sampler at the float deployment station. The long-term accuracy of the corrected data is compared to the GLODAPv2 data set whenever a float made a profile within 20 km of a GLODAPv2 station. Based on these assessments, the fleet average oxygen data are accurate to 1 ± 1%, nitrate to within 0.5 ± 0.5 µmol kg-1, and pH to 0.005 ± 0.007, where the error limit is 1 standard deviation of the fleet data. The bio-optical measurements of chlorophyll fluorescence and optical backscatter are used to estimate chlorophyll a and particulate organic carbon concentration. The particulate organic carbon concentrations inferred from optical backscatter appear accurate to with 35 mg C m-3 or 20%, whichever is larger. Factors affecting the accuracy of the estimated chlorophyll a concentrations are evaluated.
BIO ARGO floats: tools for operational monitoring of the Black Sea
NASA Astrophysics Data System (ADS)
Palazov, Atanas; Slabakova, Violeta; Peneva, Elisaveta; Stanev, Emil
2014-05-01
The assessment of ecological status in the context of the Water Framework Directive (WFD) and Marine Strategy Framework Directive (MSFD) requires comprehensive knowledge and understanding of the physical and biogeochemical processes that determine the functioning of marine ecosystems. One of the main challenges however is the need of data with frequency relevant to the spatial and temporal scales of the ecological processes. The majority of in situ observations that are commonly used for ecological monitoring of the Black Sea are generally based on near-shore monitoring programs or irregular oceanographic cruises that provide either non-synoptic, coarse resolution realizations of large scale processes or detailed, but time and site specific snapshots of local features. These gaps can be filled by two independent sources: satellite observation and profiling floats. In fact satellite ocean color sensors allows for determination at synoptic scale of water quality parameters through its absorption properties. However the satellite ocean color methods have a number of limitations such as: measurements can only be made during daylight hours; require cloud-free conditions and are sensitive to atmospheric aerosols; provide information only for the upper layer of the ocean (approximately the depth of 10% incident light); algorithms developed for global applications are a source of large uncertainties in the marginal seas and costal areas. These constrains of the optical remote sensing observations can be avoided by using miniature biogeochemical sensors and autonomous platforms that offer remarkable perspectives for observing the "biological" ocean, notably at critical spatiotemporal scales which have been out of reach until recently (Claustre et al., 2010). In the frame of "E-AIMS: Euro-Argo Improvements for the GMES marine Service" 7 EC FP project two Bio Argo floats were deployed in the Black Sea. Beside the traditionally CTD the floats were equipped with biogeochemical sensors (oxygen, irradiance, chl-a and backscattering). The selection of the deployment locations was limited only to the Bulgarian Black Sea waters, so that the optimal deployment strategy that has been chosen was the floats to be deployed in the maximum distant positions from each other along the Black Sea geostrophic current at depth ~ 1800 m. Coincident biogeochemical and in-water radiometric measurements were collected at the time of each float deployment to ensure intercalibration of the instruments mounted on the floats and as well as to find empirical relationship between optical data and biogeochemical variables. The data obtained form Bio floats will be used to: investigate the seasonal evolution of oxygen in the upper layers, including the subsurface oxygen maximum; study the seasonal and inter annual dynamics of phytoplankton blooms in the deeper Black Sea; cross validation of satellite derived Chl-a and backscattering. References: Claustre et al. (2010). Bio-optical profiling floats as new observational tools for biogeochemical and ecosystem studies: potential synergies with ocean color remote sensing. Proceedings of the "OceanObs'09: Sustained Ocean Observations and Information for Society" Conference, Venice/Italy.
NASA Astrophysics Data System (ADS)
Lamarche, Geoffroy; Lurton, Xavier
2018-06-01
Multibeam echosounders are becoming widespread for the purposes of seafloor bathymetry mapping, but the acquisition and the use of seafloor backscatter measurements, acquired simultaneously with the bathymetric data, are still insufficiently understood, controlled and standardized. This presents an obstacle to well-accepted, standardized analysis and application by end users. The Marine Geological and Biological Habitat Mapping group (Geohab.org) has long recognized the need for better coherence and common agreement on acquisition, processing and interpretation of seafloor backscatter data, and established the Backscatter Working Group (BSWG) in May 2013. This paper presents an overview of this initiative, the mandate, structure and program of the working group, and a synopsis of the BSWG Guidelines and Recommendations to date. The paper includes (1) an overview of the current status in sensors and techniques available in seafloor backscatter data from multibeam sonars; (2) the presentation of the BSWG structure and results; (3) recommendations to operators, end-users, sonar manufacturers, and software developers using sonar backscatter for seafloor-mapping applications, for best practice methods and approaches for data acquisition and processing; and (4) a discussion on the development needs for future systems and data processing. We propose for the first time a nomenclature of backscatter processing levels that affords a means to accurately and efficiently describe the data processing status, and to facilitate comparisons of final products from various origins.
Comparison of AMSR-E and SSM/I snow parameter retrievals over the Ob river basin
Mognard, N.M.; Grippa, M.; LeToan, T.; Kelly, R.E.J.; Chang, A.T.C.; Josberger, E.G.
2004-01-01
Passive microwave observations from the Advanced Microwave Scanning Radiometer - EOS (AMSR-E) and from the Special Sensor Microwave Imager (SSM/I) are used to analyse the evolution of the snow pack in the Ob river basin during the snow season of 2002-03. The Ob river is the biggest Russian river with respect to its watershed area (2 975 000 km2). The Ob originates in the Altai mountains and flows northward across the vast West Siberian lowland towards the Arctic Ocean. The majority of snow cover is contained in the lowlands rather than in mountainous regions and persists for six months or more. During the snow season, surface air temperatures are very cold. Therefore, the combination of cold dry snow and large areas of uniform topography is ideal for snowpack extent and water equivalent retrievals from passive microwave observations. The thermal gradient through the snow pack is estimated and used to model the growth of the snow grain size and to compute the evolution of the passive microwave derived snow depth over the region. A comparison between the AMSR-E and SSM/I estimates is performed and the differences between the snow parameters from the two satellite instruments are analysed.
NASA Astrophysics Data System (ADS)
hello, yann; Charvis, Philippe; Yegikyan, Manuk; verfaillie, Romain; Rivet, Diane
2016-04-01
Real time monitoring of seismic activity is a major issue for early warning of earthquakes and tsunamis. It can be done using regional scale wired nodes, such as Neptune in Canada and in the U.S, or DONET in Japan. Another approach to monitor seismic activity at sea is to deploying repeatedly OBS array like during the amphibious Cascadia Initiative (four time 1-year deployments), the Japanese Pacific Array (broadband OBSs "ocean-bottom broadband dispersion survey" with 2-years autonomy), the Obsismer program in the French Lesser Antilles (eight time 6-months deployments) and the Osisec program in Ecuador (four time 6-months deployments). These autonomous OBSs are self-recovered or recovered using an ROV. These systems are costly including ship time, and require to recover the OBS before to start working on data. Among the most recent alternative we developed a 3/4 years autonomy ocean bottom system with 9 channels (?) allowing the acquisition of different seismic or environmental parameters. MUG-OBS is a free falling instrument rated down to 6000 m. The installation of the sensor is monitored by acoustic commands from the surface and a health bulletin with data checking is recovered by acoustic during the installation. The major innovation is that it is possible to recover the data any time on demand (regularly every 6-months or after a crisis) using one of the 6 data-shuttles released from the surface by acoustic command using a one day fast cruise boat of opportunity. Since sensors stayed at the same location for 3 years, it is a perfect tool to monitor large seismic events, background seismic activity and aftershock distribution. Clock, drift measurement and GPS localization is automatic when the shuttle reaches the surface. For remote areas, shuttles released automatically and a seismic events bulletin is transmitted. Selected data can be recovered by two-way Iridium satellite communication. After a period of 3 years the main station is self-recovered by acoustic.
NASA Astrophysics Data System (ADS)
Tsekhmistrenko, Maria; Sigloch, Karin; Hosseini, Kasra; Barruol, Guilhem
2016-04-01
From 2011 to 2014, the RHUM-RUM project (Reunion Hotspot Upper Mantle - Reunions Unterer Mantel) instrumented a 2000x2000km2 area of Indian Ocean seafloor, islands and Madagascar with broadband seismometers and hydrophones. The central component was a 13-month deployment of 57 German and French Ocean Bottom Seismometers (OBS) in 2300-5600 m depth. This was supplemented by 2-3 year deployments of 37 island stations on Reunion, Mauritius, Rodrigues, the southern Seychelles, the Iles Eparses and southern Madagascar. Two partner projects contributed another 30+ stations on Madagascar. Our ultimate objective is multifrequency waveform tomography of the entire mantle column beneath the Reunion hotspot. Ideally we would use all passbands that efficiently transmit body waves but this meets practical limits in the noise characteristics of ocean-bottom recordings in particular. Here we present the preliminary data set of frequency-dependent P-wave traveltime measurements on seismometers and hydrophones, obtained by cross-correlation of observed with predicted waveforms. The latter are synthesized from fully numerical Green's functions and carefully estimated, broadband source time functions. More than 200 teleseismic events during the 13-month long deployment yielded usable P-waveform measurements. We present our methods and discuss data yield and quality of ocean-bottom versus land seismometers, and of OBS versus broadband hydrophones. Above and below the microseismic noise band, data yields are higher than within it, especially for OBS. The 48 German OBS, equipped with Guralp 60 s sensors, were afflicted by relatively high self-noise compared to the 9 French instruments equipped with Nanometrics Trillium 240 s sensors. The HighTechInc (model HTI-01 and HTI-04-PCA/ULF) hydrophones (100 s corner period) functioned particularly reliably but their waveforms are relatively more challenging to model due to reverberations in the water column. We obtain ~15000 combined cross-correlations measurements that should be usable in multifrequency P-wave tomography, in passbands between 30 s and 2.7 s dominant period.
Marginal Ice Zone: Biogeochemical Sampling with Gliders
2014-09-30
chlorophyll primary productivity model to estimate and compare phytoplankton productivity under full ice cover, in the MIZ, and in open ice-free water...September, the gliders and still operating but will be retrieved in early October from the R/V Norseman. All gliders carried sensors for chlorophyll ...program, with modification for local conditions. The specific protocols for each sensor – backscatter and chlorophyll fluorescence – are described in
Concentration Measurements of Suspended Load using ADV with Influence of the Particle Size
NASA Astrophysics Data System (ADS)
Schwarzwälder, Kordula
2017-04-01
ADV backscatter data can be used under certain conditions to gain information about the concentrations of suspended loads. This was shown in many studies before (Fugate and Friedrichs 2002; Chanson et al 2008; Ha et al. 2009). This paper reports on a pre-study to investigate the influence of particle size on concentration measurements for suspended sediment load with ADV. The study was conducted in a flume in the Oskar-von-Miller-Institute using fresh water from a river including the natural suspended load. The ADV used in the experiments was a Vectrino Profiler (Nortek). In addition water samples were taken for TSS and TOC. For the measurements a surge was generated in the flume to ensure that also particles of larger size will be present in the water phase. The measurements and samples were taken during the whole surge event. Therefore we were able to find a good correlation between the backscatter data of the ADV and the TSS as well as TOC results. For the decreasing part of the flow event the concentration of TOC in the suspended load of the water phase is decreasing much slower than the TSS and results in a damped decrease of the backscatter values. This means that the results for concentration measurements might be slightly influenced by the size of the particles. Further evaluations of measurements conducted with a LISST SL (Sequoia) will be investigated to show the trend of the particle sizes during this process and fortify this result. David C. Fugate, Carl T. Friedrichs, Determining concentration and fall velocity of estuarine particle populations using ADV, OBS and LISST, Continental Shelf Research, Volume 22, Issues 11-13, 2002 H.K. Ha, W.-Y. Hsu, J.P.-Y. Maa, Y.Y. Shao, C.W. Holland, Using ADV backscatter strength for measuring suspended cohesive sediment concentration, Continental Shelf Research, Volume 29, Issue 10, 2009 Hubert Chanson, Maiko Takeuchi, Mark Trevethan, Using turbidity and acoustic backscatter intensity as surrogate measures of suspended sediment concentration in a small subtropical estuary, Journal of Environmental Management, Volume 88, Issue 4, 2008
Distributed temperature sensor testing in liquid sodium
DOE Office of Scientific and Technical Information (OSTI.GOV)
Gerardi, Craig; Bremer, Nathan; Lisowski, Darius
Rayleigh-backscatter-based distributed fiber optic sensors were immersed in sodium to obtain high-resolution liquid-sodium temperature measurements. Distributed temperature sensors (DTSs) functioned well up to 400°C in a liquid sodium environment. The DTSs measured sodium column temperature and the temperature of a complex geometrical pattern that leveraged the flexibility of fiber optics. A single Ø 360 lm OD sensor registered dozens of temperatures along a length of over one meter at 100 Hz. We also demonstrated the capability to use a single DTS to simultaneously detect thermal interfaces (e.g. sodium level) and measure temperature.
NASA Astrophysics Data System (ADS)
Heffron, E.; Lurton, X.; Lamarche, G.; Brown, C.; Lucieer, V.; Rice, G.; Schimel, A.; Weber, T.
2015-12-01
Backscatter data acquired with multibeam sonars are now commonly used for the remote geological interpretation of the seabed. The systems hardware, software, and processing methods and tools have grown in numbers and improved over the years, yet many issues linger: there are no standard procedures for acquisition, poor or absent calibration, limited understanding and documentation of processing methods, etc. A workshop organized at the GeoHab (a community of geoscientists and biologists around the topic of marine habitat mapping) annual meeting in 2013 was dedicated to seafloor backscatter data from multibeam sonars and concluded that there was an overwhelming need for better coherence and agreement on the topics of acquisition, processing and interpretation of data. The GeoHab Backscatter Working Group (BSWG) was subsequently created with the purpose of documenting and synthetizing the state-of-the-art in sensors and techniques available today and proposing methods for best practice in the acquisition and processing of backscatter data. Two years later, the resulting document "Backscatter measurements by seafloor-mapping sonars: Guidelines and Recommendations" was completed1. The document provides: An introduction to backscatter measurements by seafloor-mapping sonars; A background on the physical principles of sonar backscatter; A discussion on users' needs from a wide spectrum of community end-users; A review on backscatter measurement; An analysis of best practices in data acquisition; A review of data processing principles with details on present software implementation; and finally A synthesis and key recommendations. This presentation reviews the BSWG mandate, structure, and development of this document. It details the various chapter contents, its recommendations to sonar manufacturers, operators, data processing software developers and end-users and its implication for the marine geology community. 1: Downloadable at https://www.niwa.co.nz/coasts-and-oceans/research-projects/backscatter-measurement-guidelines
Submarine cable OBS using a retired submarine telecommunication cable: GeO-TOC program
NASA Astrophysics Data System (ADS)
Kasahara, Junzo; Utada, Hisashi; Sato, Toshinori; Kinoshita, Hajimu
1998-06-01
In order to study the Earth's structure and subduction zone tectonics, seismic data from the oceanic region are extremely important. The present seismograph distribution in the oceanic region, however, provides a very poor coverage. To improve this poor seismic coverage, a cable OBS system using a retired submarine telecommunication cable is proposed. The GeO-TOC cable runs from Ninomiya, Japan, to Guam through the Izu-Bonin forearc and the Marina Trough. The total length of the cable is 2659 km. An OBS, IZU, using the GeO-TOC cable, was successfully installed at the landward slope of the Izu-Bonin Trench in January 1997. The IZU OBS is located approximately 400 km south of Tokyo. The installation method is similar to repair work on submarine cables. The IZU OBS is equipped with three accelerometers, a hydrophone, a quartz pressure gauge, and a quartz precision thermometer with a few temperature sensors to monitor overheating of the internal electronics. After installation, the voltage increase is 90 V when the current is maintained at a constant 370 mA. Data from accelerometers are digitized by 24-bit A/D converters and sent to Ninomiya at 9600 bps for each component. Hydrophone data are sent to Ninomiya as analog signals using the AM (Amplitude Modulation) method for safety reasons. Hydrophone data are digitized at the shore station. Other slow-rate data are multiplexed and sent to the shore at 9600 bps. The instrument can be controlled by a shore computer. All data will be transmitted from Ninomiya to Tokyo and combined with other existing seismic data.
Radar backscattering properties of corn and soybeans at frequencies of 1.6, 4.75, and 13.3. GHz
NASA Technical Reports Server (NTRS)
Paris, J. F.
1983-01-01
The NASA Johnson Space Center made an observational study of the radar-backscattering properties of corn and soybeans in commercial fields in a test site in Webster County, IA. Aircraft-based radar scatterometers measured the backscattering coefficient of the crops at three frequencies, 1.6 GHz (L-band), 4.75 GHz (C-band), and 13.3 GHz (Ku-band), at 10 sensor look-angles (5 to 50 degrees from the nadir in steps of 5 degrees), and with several polarization combinations. Among other findings, it was determined that: (1) row direction differences among fields affected significantly the radar-backscattering coefficient of the fields when the radar system used like-polarization at look-angles from 5 to 25 degrees; (2) row-direction differences had no effect on radar backscattering when the system used either cross-polarization or look-angles greater than 25 degrees regardless of the polarization; (3) wet surface-soil moisture conditions resulted in significantly poorer spectral separability of the two crops as compared to dry-soil conditions; and (4) on the dry-soil date, the best channel for separating corn from soybeans was the C-band cross-polarized measurement at a look-angle of 50 degrees.
Atmospheric aerosol measurements by employing a polarization scheimpflug lidar system
NASA Astrophysics Data System (ADS)
Mei, Liang; Guan, Peng; Yang, Yang
2018-04-01
A polarization Scheimpflug lidar system based on the Scheimpflug principle has been developed by employing a compact 808-nm multimode highpower laser diode and two highly integrated CMOS sensors in Dalian University of Technology (DLUT), Dalian, China. The parallel and orthogonal polarized backscattering signal are recorded by two 45 degree tilted image sensors, respectively. Atmospheric particle measurements were carried out by employing the polarization Scheimpflug lidar system.
An Autonomous Wireless Sensor Node With Asynchronous ECG Monitoring in 0.18 μ m CMOS.
Mansano, Andre L; Li, Yongjia; Bagga, Sumit; Serdijn, Wouter A
2016-06-01
The design of a 13.56 MHz/402 MHz autonomous wireless sensor node with asynchronous ECG monitoring for near field communication is presented. The sensor node consists of an RF energy harvester (RFEH), a power management unit, an ECG readout, a data encoder and an RF backscattering transmitter. The energy harvester supplies the system with 1.25 V and offers a power conversion efficiency of 19% from a -13 dBm RF source at 13.56 MHz. The power management unit regulates the output voltage of the RFEH to supply the ECG readout with VECG = 0.95 V and the data encoder with VDE = 0.65 V . The ECG readout comprises an analog front-end (low noise amplifier and programmable voltage to current converter) and an asynchronous level crossing ADC with 8 bits resolution. The ADC output is encoded by a pulse generator that drives a backscattering transmitter at 402 MHz. The total power consumption of the sensor node circuitry is 9.7 μ W for a data rate of 90 kb/s and a heart rate of 70 bpm. The chip has been designed in a 0.18 μm CMOS process and shows superior RF input power sensitivity and lower power consumption when compared to previous works.
NASA Astrophysics Data System (ADS)
Fonseca, Luciano; Hung, Edson Mintsu; Neto, Arthur Ayres; Magrani, Fábio José Guedes
2018-06-01
A series of multibeam sonar surveys were conducted from 2009 to 2013 around Admiralty Bay, Shetland Islands, Antarctica. These surveys provided a detailed bathymetric model that helped understand and characterize the bottom geology of this remote area. Unfortunately, the acoustic backscatter records registered during these bathymetric surveys were heavily contaminated with noise and motion artifacts. These artifacts persisted in the backscatter records despite the fact that the proper acquisition geometry and the necessary offsets and delays were applied during the survey and in post-processing. These noisy backscatter records were very difficult to interpret and to correlate with gravity-core samples acquired in the same area. In order to address this issue, a directional notch-filter was applied to the backscatter waterfall in the along-track direction. The proposed filter provided better estimates for the backscatter strength of each sample by considerably reducing residual motion artifacts. The restoration of individual samples was possible since the waterfall frame of reference preserves the acquisition geometry. Then, a remote seafloor characterization procedure based on an acoustic model inversion was applied to the restored backscatter samples, generating remote estimates of acoustic impedance. These remote estimates were compared to Multi Sensor Core Logger measurements of acoustic impedance obtained from gravity core samples. The remote estimates and the Core Logger measurements of acoustic impedance were comparable when the shallow seafloor was homogeneous. The proposed waterfall notch-filtering approach can be applied to any sonar record, provided that we know the system ping-rate and sampling frequency.
NASA Astrophysics Data System (ADS)
Riedi, J.; Labonnote, L. C.; Contaut, F.; Platnick, S. E.; Yang, P.
2016-12-01
Realistic assumptions for representation of ice crystal optical properties are key in deriving meaningful information on ice clouds from spaceborne observations. With the increasing number of multi-sensor analysis it is also of paramount importance that ice crystal models be consistents for the interpretation of both passive and active observations in the solar and thermal infrared spectral domains. There has been significant evidences in the past few years that roughened particles might represent an overall good proxy for ice crystal models being able to simultaneously explain visible and infrared observations obtained from either active or passive sensors (Holz et al, 2016). Nevertheless, details of the exact phase function remain very informative fingerprints of ice crystal shapes and can also be critical parameters for retrievals performed under specific viewing geometries. Analysis of lidar observation for instance remains very sensitive to details of phase function in and around the backscatter direction. The relative magnitude and width of the backscatter peak intensity that appears in phase functions of ice crystal has been shown to carry useful information for characterization of ice crystal habits (Zhou & Yang, 2015). Based on these theoretical results we are revisiting here our previous analysis of coincident POLDER, MODIS and CALIOP observations whereby we were able to study the angular variability of ice clouds reflectance in and around the exact backscatter direction. Statistics from 5 years of observations of peak intensities derived from POLDER have been established in relation to coincident MODIS cloud optical thickness and effective radius retrievals as well as CALIOP layer integrated depolarization ratio and attenuated backscatter. Those are analyzed in view of the theoretical results from Zhou & Yang (2015). In particular, correlation of peak intensity and width with particle size retrieved from MODIS will be presented and implications for ice cloud microphysical properties and remote sensing applications will be discussed. Chen Zhou and Ping Yang : Backscattering peak of ice cloud particles, Opt. Express 23, 11995-12003 (2015) Holz, R. E. et al : Resolving ice cloud optical thickness biases between CALIOP and MODIS using infrared retrievals, Atmos. Chem. Phys., 16, 5075-5090 (2016)
Active pixel sensor array as a detector for electron microscopy.
Milazzo, Anna-Clare; Leblanc, Philippe; Duttweiler, Fred; Jin, Liang; Bouwer, James C; Peltier, Steve; Ellisman, Mark; Bieser, Fred; Matis, Howard S; Wieman, Howard; Denes, Peter; Kleinfelder, Stuart; Xuong, Nguyen-Huu
2005-09-01
A new high-resolution recording device for transmission electron microscopy (TEM) is urgently needed. Neither film nor CCD cameras are systems that allow for efficient 3-D high-resolution particle reconstruction. We tested an active pixel sensor (APS) array as a replacement device at 200, 300, and 400 keV using a JEOL JEM-2000 FX II and a JEM-4000 EX electron microscope. For this experiment, we used an APS prototype with an area of 64 x 64 pixels of 20 microm x 20 microm pixel pitch. Single-electron events were measured by using very low beam intensity. The histogram of the incident electron energy deposited in the sensor shows a Landau distribution at low energies, as well as unexpected events at higher absorbed energies. After careful study, we concluded that backscattering in the silicon substrate and re-entering the sensitive epitaxial layer a second time with much lower speed caused the unexpected events. Exhaustive simulation experiments confirmed the existence of these back-scattered electrons. For the APS to be usable, the back-scattered electron events must be eliminated, perhaps by thinning the substrate to less than 30 microm. By using experimental data taken with an APS chip with a standard silicon substrate (300 microm) and adjusting the results to take into account the effect of a thinned silicon substrate (30 microm), we found an estimate of the signal-to-noise ratio for a back-thinned detector in the energy range of 200-400 keV was about 10:1 and an estimate for the spatial resolution was about 10 microm.
NASA Technical Reports Server (NTRS)
Fenner, R. G.; Reid, S. C.; Solie, C. H.
1980-01-01
An evaluation is given of how active and passive microwave sensors can best be used in oil spill detection and assessment. Radar backscatter curves taken over oil spills are presented and their effect on synthetic aperture radar (SAR) imagery are discussed. Plots of microwave radiometric brightness variations over oil spills are presented and discussed. Recommendations as to how to select the best combination of frequency, viewing angle, and sensor type for evaluation of various aspects of oil spills are also discussed.
NASA Technical Reports Server (NTRS)
Jarzembski, Maurice A.; Srivastava, Vandana; Goodman, H. Michael (Technical Monitor)
2000-01-01
Airborne lidar systems are used to determine wind velocity and to measure aerosol or cloud backscatter variability. Atmospheric aerosols, being affected by local and regional sources, show tremendous variability. Continuous wave (cw) lidar can obtain detailed aerosol loading with unprecedented high resolution (3 sec) and sensitivity (1 mg/cubic meter) as was done during the 1995 NASA Multi-center Airborne Coherent Atmospheric Wind Sensor (MACAWS) mission over western North America and the Pacific Ocean. Backscatter variability was measured at a 9.1 micron wavelength cw focused CO2 Doppler lidar for approximately 52 flight hours, covering an equivalent horizontal distance of approximately 30,000 km in the troposphere. Some quasi-vertical backscatter profiles were also obtained during various ascents and descents at altitudes that ranged from approximately 0.1 to 12 km. Similarities and differences for aerosol loading over land and ocean were observed. Mid-tropospheric aerosol backscatter background mode was approximately 6 x 10(exp -11)/ms/r, consistent with previous lidar datasets. While these atmospheric measurements were made, the lidar also retrieved a distinct backscatter signal from the Earth's surface from the unfocused part of the focused cw lidar beam during aircraft rolls. Atmospheric backscatter can be highly variable both spatially and temporally, whereas, Earth-surface backscatter is relatively much less variant and can be quite predictable. Therefore, routine atmospheric backscatter measurements by an airborne lidar also give Earth surface backscatter which can allow for investigating the Earth terrain. In the case where the Earth's surface backscatter is coming from a well-known and fairly uniform region, then it can potentially offer lidar calibration opportunities during flight. These Earth surface measurements over varying Californian terrain during the mission were compared with laboratory backscatter measurements using the same lidar of various Earth surfaces giving good agreement, suggesting that the lidar efficiency, and thus a lidar calibration factor for detection, can be estimated fairly well using Earth's surface signal.
Hyperspectral image analysis for standoff trace detection using IR laser spectroscopy
NASA Astrophysics Data System (ADS)
Jarvis, J.; Fuchs, F.; Hugger, S.; Ostendorf, R.; Butschek, L.; Yang, Q.; Dreyhaupt, A.; Grahmann, J.; Wagner, J.
2016-05-01
In the recent past infrared laser backscattering spectroscopy using Quantum Cascade Lasers (QCL) emitting in the molecular fingerprint region between 7.5 μm and 10 μm proved a highly promising approach for stand-off detection of dangerous substances. In this work we present an active illumination hyperspectral image sensor, utilizing QCLs as spectral selective illumination sources. A high performance Mercury Cadmium Telluride (MCT) imager is used for collection of the diffusely backscattered light. Well known target detection algorithms like the Adaptive Matched Subspace Detector and the Adaptive Coherent Estimator are used to detect pixel vectors in the recorded hyperspectral image that contain traces of explosive substances like PETN, RDX or TNT. In addition we present an extension of the backscattering spectroscopy technique towards real-time detection using a MOEMS EC-QCL.
Comparison of SeaWinds Backscatter Imaging Algorithms
Long, David G.
2017-01-01
This paper compares the performance and tradeoffs of various backscatter imaging algorithms for the SeaWinds scatterometer when multiple passes over a target are available. Reconstruction methods are compared with conventional gridding algorithms. In particular, the performance and tradeoffs in conventional ‘drop in the bucket’ (DIB) gridding at the intrinsic sensor resolution are compared to high-spatial-resolution imaging algorithms such as fine-resolution DIB and the scatterometer image reconstruction (SIR) that generate enhanced-resolution backscatter images. Various options for each algorithm are explored, including considering both linear and dB computation. The effects of sampling density and reconstruction quality versus time are explored. Both simulated and actual data results are considered. The results demonstrate the effectiveness of high-resolution reconstruction using SIR as well as its limitations and the limitations of DIB and fDIB. PMID:28828143
High Resolution Near-Bed Observations in Winter Near Cape Hatteras, North Carolina
2010-06-01
Druck pressure sensors, Campbell optical backscatter, and Seatech or Wetlabs CSTAR transmission sensors. All the transmissometers were 25 cm path...14.0 m Depth Flobee Tripods Sontek hydra Acoustic Doppler velocimeter (ADV), thermistor 3D flow velocity, temperature 8 Hz for 17.5 min hourly Pulse...coherent acoustic Doppler profiler (PCADP), thermistor Profiles of 3D flow velocity, temperature 1 Hz for 17.5 min hourly, 6.3 cm bins Pressure
NASA Technical Reports Server (NTRS)
Srivastava, V.; Rothermel, J.; Jarzembski, M. A.; Clarke, A. D.; Cutten, D. R.; Bowdle, D. A.; Spinhirne, J. D.; Menzies, R. T.
1999-01-01
Space-based and airborne coherent Doppler lidars designed for measuring global tropospheric wind profiles in cloud-free air rely on backscatter, beta from aerosols acting as passive wind tracers. Aerosol beta distribution in the vertical can vary over as much as 5-6 orders of magnitude. Thus, the design of a wave length-specific, space-borne or airborne lidar must account for the magnitude of 8 in the region or features of interest. The SPAce Readiness Coherent Lidar Experiment under development by the National Aeronautics and Space Administration (NASA) and scheduled for launch on the Space Shuttle in 2001, will demonstrate wind measurements from space using a solid-state 2 micrometer coherent Doppler lidar. Consequently, there is a critical need to understand variability of aerosol beta at 2.1 micrometers, to evaluate signal detection under varying aerosol loading conditions. Although few direct measurements of beta at 2.1 micrometers exist, extensive datasets, including climatologies in widely-separated locations, do exist for other wavelengths based on CO2 and Nd:YAG lidars. Datasets also exist for the associated microphysical and chemical properties. An example of a multi-parametric dataset is that of the NASA GLObal Backscatter Experiment (GLOBE) in 1990 in which aerosol chemistry and size distributions were measured concurrently with multi-wavelength lidar backscatter observations. More recently, continuous-wave (CW) lidar backscatter measurements at mid-infrared wavelengths have been made during the Multicenter Airborne Coherent Atmospheric Wind Sensor (MACAWS) experiment in 1995. Using Lorenz-Mie theory, these datasets have been used to develop a method to convert lidar backscatter to the 2.1 micrometer wavelength. This paper presents comparison of modeled backscatter at wavelengths for which backscatter measurements exist including converted beta (sub 2.1).
Rojas-Líbano, Daniel; Frederick, Donald E.; Egaña, José I.; Kay, Leslie M.
2014-01-01
Sensory-motor relationships are part of the normal operation of sensory systems. Sensing occurs in the context of active sensor movement, which in turn influences sensory processing. We address such a process in the rat olfactory system. Through recordings of the diaphragm electromyogram (EMG), we monitored the motor output of the respiratory circuit involved in sniffing behavior, simultaneously with the local field potential (LFP) of the olfactory bulb (OB) in rats moving freely in a familiar environment, where they display a wide range of respiratory frequencies. We show that the OB LFP represents the sniff cycle with high reliability at every sniff frequency and can therefore be used to study the neural representation of motor drive in a sensory cortex. PMID:24966821
Hydrodynamics Offshore of the North Beach of Indian River Inlet, DE
NASA Astrophysics Data System (ADS)
DiCosmo, N. R.; Puleo, J. A.
2014-12-01
The Indian River Inlet (IRI) on the east coast of Delaware, USA connects the Atlantic Ocean to the Indian River and Rehoboth Bays. Long-term and large-scale net alongshore sediment transport along this portion of coastline is from south to north. The north beach of IRI suffers from severe erosion due to interruption of the alongshore transport and current variability near the inlet. The magnitude of such erosion has increased over the past decade and questions have arisen as to the cause. The goal of this study is to quantify currents and wave patterns and estimate sediment transport rates at the north beach and near the inlet in an effort to determine the causes of persistent erosion. Data were obtained from October 2013 to March 2014 in the form of 3 separate 28-day deployments. Each deployment consisted of 4 proposed deployment sites. Data at each site were collected using a bottom mounted Nortek Aquadopp Acoustic Doppler Current Profiler (ADCP) and 2 Campbell Scientific Optical Backscatter Sensors (OBS). Currents and OBS data were sampled every 120 s. Waves were sampled for approximately 17 minutes at the beginning of every hour. Data analysis from the deployments indicates the presence of several interesting trends in currents that can be linked to the persistent erosion. Current data are filtered to quantify typical current speed and direction for a tidal cycle (peak flood to peak flood) at each deployment site. The typical currents off of the north beach and up to 800 m north of the north jetty are mostly directed southward over the entire tidal cycle. This consistent southward flow implies: 1) there is no flow reversal based on tide, contrary to what might be expected at an inlet adjacent beach, 2) the typical current direction is opposite of the expectations for the known long-term large-scale net alongshore transport and 3) the consistency of this atypical current may be responsible for transporting sediment southward and away from the north beach. Currents and waves will be further analyzed for storm and non-storm conditions in order to more completely quantify the hydrodynamics of the area. Sediment data will also be analyzed in conjunction with the hydrodynamic data in order to better understand the sediment transport process.
NASA Astrophysics Data System (ADS)
Nuñez-Cornu, F. J.; Barba, D. C., Sr.; Danobeitia, J.; Bandy, W. L.; Zamora-Camacho, A.; Marquez-Ramirez, V. H.; Ambros, M.; Gomez, A.; Sandoval, J. M.; Mortera-Gutierrez, C. A.
2016-12-01
The second stage of TsuJal Project includes the study of passive seismic activity in the region of the plate Rivera and Jalisco block by anchoring OBS and densifying the network of seismic stations on land for at least four months. This stage began in April 2016 with the deployment of 25 Obsidian stations with sensor Le-3D MkIII from the northern part of Nayarit state to the south of Colima state, including the Marias Islands. This temporal seismic network complements the Jalisco Seismic Network (RESAJ) for a total of 50 stations. Offshore, ten OBS type LCHEAPO 2000 with 4 channel (3 seismic short period and 1 pressure) were deployed, in the period from 19 to 30 April 2016 using the BO El Puma from UNAM. The OBS were deployed in an array from the Marias Islands to offcoast of the border of Colima and Michoacan states. On May 4, an earthquake with Ml = 4.2 took place in the contact area of the Rivera Plate, Cocos Plate and the Middle America Trench, subsequently occurred a seismic swarm with over 200 earthquakes until May 16, including an earthquake with Ml = 5.0 on May 7. A second swarm took place between May 28 and Jun 4 including an earthquake with Ml = 4.8 on Jun 1. An analysis of the quality of different location methods is presented: automatic preliminary RESAJ location using Antelope; location with revised RESAJ phases in Antelope; relocation of RESAJ data with hypo and a regional velocity model; relocation of RESAJ data with hypo adding data from the temporal seismic network stations; and finally the relocation adding the data from the OBS network. Moreover, the tectonic implications of these earthquakes are discussed.
Nieto, Gema; Xiong, Youling L; Payne, Fred; Castillo, Manuel
2015-02-01
The objective of this study was to determine whether light backscatter response from fresh pork meat emulsions is correlated to final product stability indices. A specially designed fiber optic measurement system was used in combination with a miniature fiber optic spectrometer to determine the intensity of light backscatter within the wavelength range 300-1100 nm (UV/VIS/NIR) at different radial distances (2, 2.5 and 3mm) with respect to the light source in pork meat emulsions with two fat levels (15%, 30%) and two levels (0, 2.5%) of the natural antioxidant hydrolyzed potato protein (HPP). Textural parameters (hardness, deformability, cohesiveness and breaking force), cooking loss, TBARS (1, 2, 3, and 7 days of storage at 4 °C) and CIELAB color coordinates of cooked emulsions were measured. The light backscatter was directly correlated with cooking losses, color, breaking force and TBARS. The optical configuration proposed would compensate for the emulsion heterogeneity, maximizing the existing correlation between the optical signal and the emulsion quality metrics.
Study of Optical Fiber Sensors for Cryogenic Temperature Measurements.
De Miguel-Soto, Veronica; Leandro, Daniel; Lopez-Aldaba, Aitor; Beato-López, Juan Jesus; Pérez-Landazábal, José Ignacio; Auguste, Jean-Louis; Jamier, Raphael; Roy, Philippe; Lopez-Amo, Manuel
2017-11-30
In this work, the performance of five different fiber optic sensors at cryogenic temperatures has been analyzed. A photonic crystal fiber Fabry-Pérot interferometer, two Sagnac interferometers, a commercial fiber Bragg grating (FBG), and a π-phase shifted fiber Bragg grating interrogated in a random distributed feedback fiber laser have been studied. Their sensitivities and resolutions as sensors for cryogenic temperatures have been compared regarding their advantages and disadvantages. Additionally, the results have been compared with the given by a commercial optical backscatter reflectometer that allowed for distributed temperature measurements of a single mode fiber.
Distributed optical fiber dynamic magnetic field sensor based on magnetostriction.
Masoudi, Ali; Newson, Trevor P
2014-05-01
A distributed optical fiber sensor is introduced which is capable of quantifying multiple magnetic fields along a 1 km sensing fiber with a spatial resolution of 1 m. The operation of the proposed sensor is based on measuring the magnetorestrictive induced strain of a nickel wire attached to an optical fiber. The strain coupled to the optical fiber was detected by measuring the strain-induced phase variation between the backscattered Rayleigh light from two segments of the sensing fiber. A magnetic field intensity resolution of 0.3 G over a bandwidth of 50-5000 Hz was demonstrated.
Study of Optical Fiber Sensors for Cryogenic Temperature Measurements
Leandro, Daniel; Lopez-Aldaba, Aitor; Beato-López, Juan Jesus; Pérez-Landazábal, José Ignacio; Auguste, Jean-Louis; Jamier, Raphael; Roy, Philippe; Lopez-Amo, Manuel
2017-01-01
In this work, the performance of five different fiber optic sensors at cryogenic temperatures has been analyzed. A photonic crystal fiber Fabry-Pérot interferometer, two Sagnac interferometers, a commercial fiber Bragg grating (FBG), and a π-phase shifted fiber Bragg grating interrogated in a random distributed feedback fiber laser have been studied. Their sensitivities and resolutions as sensors for cryogenic temperatures have been compared regarding their advantages and disadvantages. Additionally, the results have been compared with the given by a commercial optical backscatter reflectometer that allowed for distributed temperature measurements of a single mode fiber. PMID:29189755
Evaluation of ship-based sediment flux measurements by ADCPs in tidal flows
NASA Astrophysics Data System (ADS)
Becker, Marius; Maushake, Christian; Grünler, Steffen; Winter, Christian
2017-04-01
In the past decades acoustic backscatter calibration developed into a frequently applied technique to measure fluxes of suspended sediments in rivers and estuaries. Data is mainly acquired using single-frequency profiling devices, such as ADCPs. In this case, variations of acoustic particle properties may have a significant impact on the calibration with respect to suspended sediment concentration, but associated effects are rarely considered. Further challenges regarding flux determination arise from incomplete vertical and lateral coverage of the cross-section, and the small ratio of the residual transport to the tidal transport, depending on the tidal prism. We analyzed four sets of 13h cross-sectional ADCP data, collected at different locations in the range of the turbidity zone of the Weser estuary, North Sea, Germany. Vertical LISST, OBS and CTD measurements were taken very hour. During the calibration sediment absorption was taken into account. First, acoustic properties were estimated using LISST particle size distributions. Due to the tidal excursion and displacement of the turbidity zone, acoustic properties of particles changed during the tidal cycle, at all locations. Applying empirical functions, the lowest backscattering cross-section and highest sediment absorption coefficient were found in the center of the turbidity zone. Outside the tidally averaged location of the turbidity zone, changes of acoustic parameters were caused mainly by advection. In the turbidity zone, these properties were also affected by settling and entrainment, inducing vertical differences and systematic errors in concentration. In general, due to the iterative correction of sediment absorption along the acoustic path, local errors in concentration propagate and amplify exponentially. Based on reference concentration obtained from water samples and OBS data, we quantified these errors and their effect on cross-sectional averaged concentration and sediment flux. We found that errors are effectively decreased by applying calibration parameters interpolated in time, and by an optimization of the sediment absorption coefficient. We further discuss practical aspects of residual flux determination in tidal environments and of measuring strategies in relation to site-specific tidal dynamics.
NASA Astrophysics Data System (ADS)
Perry, M. J.; Lee, C.; Rainville, L.; Cetinic, I.; Yang, E. J.; Kang, S. H.
2016-02-01
In late summer 2014 during the Marginal Ice Zone (MIZ) Experiment, an international project sponsored by ONR, four Seagliders transited open water, through the marginal ice zone, and under ice-covered regions in the Beaufort Sea, penetrating as far as 100 km into the ice pack. The gliders navigated either by GPS in open water or, when under the ice, by acoustics from sound sources embedded in the MIZ autonomous observing array. The glider sensor suite included temperature, temperature microstructure, salinity, oxygen, chlorophyll fluorescence, optical backscatter, and multi-spectral downwelling irradiance. Cruises on the IBRV Araon operating in the open Beaufort Sea and on the R/V Ukpik and Norseman operating in continental shelf waters off Alaska's north slope allowed us to construct proxy libraries for converting chlorophyll fluorescence to chlorophyll concentration and optical backscatter to particulate organic carbon concentration. Water samples were collected for chlorophyll and particulate organic carbon analysis on the cruises and aligned with optical profiles of fluorescence and backscatter using sensors that were factory calibrated at the same time as the glider sensors. Fields of chlorophyll, particulate organic carbon, light, and primary productivity are constructed from the glider data. Productivity is modeled as a function of chlorophyll and light, using photosynthesis-light (PE) models with available PE parameters from Arctic measurements. During August the region under the ice was characterized by a deep chlorophyll maximum layer with low rates of production in overlying waters. A phytoplankton bloom developed in open water at the end of September, preceding the rapid reformation of ice, despite shorter days and reduce irradiation.
DC-8 Scanning Lidar Characterization of Aircraft Contrails and Cirrus Clouds
NASA Technical Reports Server (NTRS)
Uthe, Edward E.; Nielsen, Norman B.; Oseberg, Terje E.
1998-01-01
An angular-scanning large-aperture (36 cm) backscatter lidar was developed and deployed on the NASA DC-8 research aircraft as part of the SUCCESS (Subsonic Aircraft: Contrail and Cloud Effects Special Study) program. The lidar viewing direction could be scanned continuously during aircraft flight from vertically upward to forward to vertically downward, or the viewing could be at fixed angles. Real-time pictorial displays generated from the lidar signatures were broadcast on the DC-8 video network and used to locate clouds and contrails above, ahead of, and below the DC-8 to depict their spatial structure and to help select DC-8 altitudes for achieving optimum sampling by onboard in situ sensors. Several lidar receiver systems and real-time data displays were evaluated to help extend in situ data into vertical dimensions and to help establish possible lidar configurations and applications on future missions. Digital lidar signatures were recorded on 8 mm Exabyte tape and generated real-time displays were recorded on 8mm video tape. The digital records were transcribed in a common format to compact disks to facilitate data analysis and delivery to SUCCESS participants. Data selected from the real-time display video recordings were processed for publication-quality displays incorporating several standard lidar data corrections. Data examples are presented that illustrate: (1) correlation with particulate, gas, and radiometric measurements made by onboard sensors, (2) discrimination and identification between contrails observed by onboard sensors, (3) high-altitude (13 km) scattering layer that exhibits greatly enhanced vertical backscatter relative to off-vertical backscatter, and (4) mapping of vertical distributions of individual precipitating ice crystals and their capture by cloud layers. An angular scan plotting program was developed that accounts for DC-8 pitch and velocity.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Pal, Sambit Bikas; Haldar, Arijit; Roy, Basudev
A photonic force microscope comprises of an optically trapped micro-probe and a position detection system to track the motion of the probe. Signal collection for motion detection is often carried out using the backscattered light off the probe-however, this mode has problems of low S/N due to the small backscattering cross sections of the micro-probes typically used. The position sensors often used in these cases are quadrant photodetectors. To ensure maximum sensitivity of such detectors, it would help if the detector size matched with the detection beam radius after the condenser lens (which for backscattered detection would be the trappingmore » objective itself). To suit this condition, we have used a miniature displacement sensor whose dimensions makes it ideal to work with 1:1 images of micrometer-sized trapped probes in the backscattering detection mode. The detector is based on the quadrant photo-integrated chip in the optical pick-up head of a compact disc player. Using this detector, we measured absolute displacements of an optically trapped 1.1 {mu}m probe with a resolution of {approx}10 nm for a bandwidth of 10 Hz at 95% significance without any sample or laser stabilization. We characterized our optical trap for different sized probes by measuring the power spectrum for each probe to 1% accuracy, and found that for 1.1 {mu}m diameter probes, the noise in our position measurement matched the thermal resolution limit for averaging times up to 10 ms. We also achieved a linear response range of around 385 nm with cross talk between axes {approx_equal}4% for 1.1 {mu}m diameter probes. The detector has extremely high bandwidth (few MHz) and low optical power threshold-other factors that can lead to its widespread use in photonic force microscopy.« less
Estimation of Soil Moisture with L-band Multi-polarization Radar
NASA Technical Reports Server (NTRS)
Shi, J.; Chen, K. S.; Kim, Chung-Li Y.; Van Zyl, J. J.; Njoku, E.; Sun, G.; O'Neill, P.; Jackson, T.; Entekhabi, D.
2004-01-01
Through analyses of the model simulated data-base, we developed a technique to estimate surface soil moisture under HYDROS radar sensor (L-band multi-polarizations and 40deg incidence) configuration. This technique includes two steps. First, it decomposes the total backscattering signals into two components - the surface scattering components (the bare surface backscattering signals attenuated by the overlaying vegetation layer) and the sum of the direct volume scattering components and surface-volume interaction components at different polarizations. From the model simulated data-base, our decomposition technique works quit well in estimation of the surface scattering components with RMSEs of 0.12,0.25, and 0.55 dB for VV, HH, and VH polarizations, respectively. Then, we use the decomposed surface backscattering signals to estimate the soil moisture and the combined surface roughness and vegetation attenuation correction factors with all three polarizations.
Spring Blooms Observed with Biochemical Profiling Floats from a Chemical and Biological Perspective
NASA Astrophysics Data System (ADS)
Plant, J. N.; Johnson, K. S.; Sakamoto, C.; Jannasch, H. W.; Coletti, L. J.; Elrod, V.
2015-12-01
Recently there has been renewed interest in the mechanisms which control the seasonal increases in plankton biomass (spring blooms). Changes in physical and chemical forcing (light, wind, heat and nutrients) may increase the specific growth rate of phytoplankton. These changes may also shift the predator - prey relationships within the food web structure, which can alter the balance between plankton growth and loss rates. Biogeochemical profiling floats provide a means to observe the seasonal evolution of spring blooms from a physical, chemical and biological perspective in near real time. Floats equipped with optical sensors to measure nitrate, oxygen, chlorophyll fluorescence, and optical backscatter now have a presence in many ocean regions including the North Pacific, Subarctic Pacific, North Atlantic, South Atlantic and the Southern Ocean. Data from these regions are used to compare and contrast the evolution of spring blooms. The evolution of the bloom is examined using both chemical (oxygen, nitrate) and biooptical (phytoplankton from chlorophyll fluorescence and particulate organic carbon from optical backscatter) sensors under vastly different environmental conditions.
First deployment of a Multiparameter Ocean Bottom System in the Mediterranean sea
NASA Astrophysics Data System (ADS)
Hello, Y.; Charvis, P.; Yegikyan, M.; Rivet, D.; Deschamps, A.
2016-12-01
Continuous monitoring of oceans is the next great scientific and technical challenge. Recently several cabled sea bottom observatories were developed in Canada (Neptune), in Japan (DONET) and in France (Antares) for real time monitoring of seismic activity, dynamics of the water column, global environmental changes, observation of marine life, the detection of neutrinos, etc. Nevertheless, these initiatives are costly to install and to maintain and it is unlikely that we can deploy many similar observatories in the near future. Among the most recent alternative we developed a 3-years autonomy system equipped with a Nanometrics Trillium 120 s, a triaxial accelerometer, a differential and an absolute pressure gauge, and a hydrophone. MUG-OBS (Multiparameter Geophysical Ocean Bottom system) is a free falling instrument rated down to 6000 m for the monitoring of geophysical signals (earthquakes, submarine landslides, other transient signals, …). The major innovation is that it is possible to recover the data any time on demand (regularly every 6-months or after a crisis) using one of the 6 data-shuttles released from the surface by acoustic command. The MUG-OBS prototype was tested in situ twice for a short period of one week in the bay of Villefranche-sur-mer (French Riviera) in November 2015 and March 2016. During these periods we were lucky to record several teleseismic events with an excellent signal to noise ratio. In September 2016 the instrument will be deployed in the Ligurian sea, 35 miles offshore the city of Nice, for a three-years period, to extend offshore the regional land seismic network. We will present the first seismic signals available from this new instrument. MUG-OBS is a user friendly design and once installed can be maintained by non-specialists to recover data using ship of opportunity. This make it a good challenger to be deployed along the subduction of South America in the frame of the future Subduction Zone Observatory. Such a sea bottom system could also be equipped with other sensors (ADCP, underwater Methane sensor, CO2 flow-through sensor,…) and could be used as a multidisciplinary platform for the environmental monitoring of the deep Ocean.
"Data like a movie instead of a photo” - continuous water sensor data collection, 2015 Lake MI CSMI
During the 2015 Lake Michigan CSMI effort we collected continuous undulating tow and autonomous glider data including conductivity, temperature, depth, fluorescence, and backscatter across the northern half of Lake Michigan. During the same period, we also collected discreet vert...
NASA Astrophysics Data System (ADS)
Latini, Daniele; Del Frate, Fabio; Jones, Cathleen E.
2014-10-01
SAR instruments with polarimetric capabilities, high resolution and short revisit time can provide powerful support in oil spill monitoring and different techniques of analysis have been developed for this purpose [1][2]. An oil film on the sea surface results in darker areas in SAR images, but careful interpretation is required because dark spots can also be caused by natural phenomena. In view of the very low backscatter from slicks, the Noise Equivalent Sigma Zero (NESZ) is a primary sensor parameter to be considered when using a sensor for slick analysis. Among the existing full polarimetric sensors, the high resolution and very low NESZ values of UAVSAR (L-band) and RADARSAT-2 (C-band) make them preferable for oil spill analysis compared to the last generation SAR instruments. The Deepwater Horizon disaster that occurred in the Gulf of Mexico in 2010 represents a unique and extensive test site where large amounts of SAR imagery and ground validation data are available. By applying the Cloude-Pottier decomposition method to full polarimetric UAVSAR (L-band) and RADARSAT-2 (C-band), it is possible to extract parameters that describe the scattering mechanism of the target. By comparing quasi-simultaneous acquisitions and exploiting the different penetration capabilities of the sensors, we investigate the potential of full polarimetric SAR to discriminate oil on the sea surface from look-alike phenomena covering the full range of backscattering values down to those at the instrument noise floor.
Spiral passive electromagnetic sensor (SPES) for smart sensing and de-icing
NASA Astrophysics Data System (ADS)
Iervolino, Onorio; Meo, Michele
2015-04-01
The objective of this work was to develop a wireless Spiral Passive Electromagnetic Sensor (SPES) to monitor the complex permittivity of a surrounding medium. The sensor is a self-resonating planar pattern of electrically conductive material. Investigation were conducted to demonstrate the capability of the SPES to monitor humidity and temperature gradients, and acting as an ice protection tool. An oscillating signal is used to interrogate remotely the sensor with a single loop antenna or wiring it directly to a spectrum analyser and monitoring the backscattering signal. The excited sensor responds with its own resonant frequency, amplitude and bandwidth that can be correlated to physical quantities to be monitored. Our studies showed the capability of the sensor to monitor temperature and humidity changes in composite materials and uniformly produce induction heating when the conductive path is activated by an external electric power supply that can be used for deicing of aircraft structures.
Ridge-Runnel and Swash Dynamics Field Experiment on a Steep Meso-Tidal Beach
NASA Astrophysics Data System (ADS)
Figlus, J.; Song, Y.-K.; Chardon-Maldonado, P.; Puleo, J. A.
2014-12-01
Ridge-runnel (RR) systems are morphological features that may form in the intermittently wet and dry zone of the beach immediately after storm events. Their onshore migration provides a natural way of recovery for an eroded beach but the detailed swash interactions and complex feedback mechanisms between wave dynamics, sediment transport and profile evolution are not well understood and challenging to measure in-situ. During a storm, elevated water levels and large waves can significantly erode the beach profile in a matter of hours through offshore-directed sediment transport. The beach recovery process, on the other hand, occurs over a much longer time period during less intense wave conditions. In the beginning of this 3-week field campaign at South Bethany Beach, Delaware, a Nor'easter, eroded significant portions of this steep, meso-tidal beach and formed a pronounced RR system which then evolved during the less energetic conditions after the storm. An extensive cross-shore array of sensors was installed immediately after the storm measuring near-bed velocity profiles (5 Nortek Vectrino Profilers) and horizontal velocities (6 Sontec Electromagnetic Current Meters; 1 side-looking Nortek Vectrino) suspended sediment concentrations (10 Optical Backscatter Sensors OBS-3+), and pressure fluctuations (7 GE Druck pressure transducers) in the swash zone. Dense topography surveys of the RR system were conducted twice a day during low tide conditions with a Leica RTK GPS rover system. In addition, sediment grab samples along the entire RR cross-section were collected daily. An offshore ADCP with surface wave tracking capability (Nortek 2MHz AWAC AST) measured directional wave spectra and current profiles at a water depth of approximately 6m. The RR system showed rapid onshore migration over the two tide cycles immediately after the storm, followed by a period of vertical ridge accretion of up to 3 ft at certain locations. A first look at the collected data and analysis results related to the feedback mechanisms between wave forcing and RR evolution is presented along with a discussion of difficulties encountered during the experiment.
Obesity induced alterations in redox homeostasis and oxidative stress are present from an early age.
Lechuga-Sancho, Alfonso M; Gallego-Andujar, David; Ruiz-Ocaña, Pablo; Visiedo, Francisco M; Saez-Benito, Ana; Schwarz, Mónica; Segundo, Carmen; Mateos, Rosa M
2018-01-01
Oxidative stress and inflammation have been postulated as underlying mechanisms for the development of obesity-related insulin resistance. This association however, remains elusive especially in childhood. We sought to investigate this relation by measuring oxidative stress and antioxidant response biomarkers, before and during an oral glucose tolerance test (OGTT), in different biological samples from obese children. 24 children were recruited for the study, (18 obese and 6 controls). After OGTT, the obese group was subdivided in two, according to whether or not carbohydrate metabolic impairment (Ob.IR+, Ob.IR-; respectively) was found. Different biomarkers were analyzed after fasting (T = 0) and during an OGTT (T = 60 and 120 min). Lipoperoxides were measured in plasma, erythrocytes, and urine; while advanced glycation end products were determined in plasma, and redox status (GSH/GSSG ratio) in erythrocytes. We found marked differences in the characterization of the oxidative status in urine and erythrocytes, and in the dynamics of the antioxidant response during OGTT. Specifically, Ob.IR+ children show increased oxidative stress, deficient antioxidant response and a significant imbalance in redox status, in comparison to controls and Ob.IR- children. Obese children with insulin resistance show increased levels of oxidative stress biomarkers, and a stunted antioxidant response to an OGTT leading to increased oxidative stress after a single glucose load, as detected in erythrocytes, but not in plasma. We propose erythrocytes as sensors of early and acute changes in oxidative stress associated with insulin resistance in childhood obesity. This is a pilot study, performed with a limited sample size, so data should be interpreted with caution until reproduced.
de Albuquerque, Victor Hugo C.; Barbosa, Cleisson V.; Silva, Cleiton C.; Moura, Elineudo P.; Rebouças Filho, Pedro P.; Papa, João P.; Tavares, João Manuel R. S.
2015-01-01
Secondary phases, such as laves and carbides, are formed during the final solidification stages of nickel-based superalloy coatings deposited during the gas tungsten arc welding cold wire process. However, when aged at high temperatures, other phases can precipitate in the microstructure, like the γ” and δ phases. This work presents an evaluation of the powerful optimum path forest (OPF) classifier configured with six distance functions to classify background echo and backscattered ultrasonic signals from samples of the inconel 625 superalloy thermally aged at 650 and 950 °C for 10, 100 and 200 h. The background echo and backscattered ultrasonic signals were acquired using transducers with frequencies of 4 and 5 MHz. The potentiality of ultrasonic sensor signals combined with the OPF to characterize the microstructures of an inconel 625 thermally aged and in the as-welded condition were confirmed by the results. The experimental results revealed that the OPF classifier is sufficiently fast (classification total time of 0.316 ms) and accurate (accuracy of 88.75% and harmonic mean of 89.52) for the application proposed. PMID:26024416
de Albuquerque, Victor Hugo C; Barbosa, Cleisson V; Silva, Cleiton C; Moura, Elineudo P; Filho, Pedro P Rebouças; Papa, João P; Tavares, João Manuel R S
2015-05-27
Secondary phases, such as laves and carbides, are formed during the final solidification stages of nickel-based superalloy coatings deposited during the gas tungsten arc welding cold wire process. However, when aged at high temperatures, other phases can precipitate in the microstructure, like the γ'' and δ phases. This work presents an evaluation of the powerful optimum path forest (OPF) classifier configured with six distance functions to classify background echo and backscattered ultrasonic signals from samples of the inconel 625 superalloy thermally aged at 650 and 950 °C for 10, 100 and 200 h. The background echo and backscattered ultrasonic signals were acquired using transducers with frequencies of 4 and 5 MHz. The potentiality of ultrasonic sensor signals combined with the OPF to characterize the microstructures of an inconel 625 thermally aged and in the as-welded condition were confirmed by the results. The experimental results revealed that the OPF classifier is sufficiently fast (classification total time of 0.316 ms) and accurate (accuracy of 88.75%" and harmonic mean of 89.52) for the application proposed.
Contextual Awareness for Robust Robot Autonomy
2013-12-30
are some ob - stacles that are either undetectable or only partially detectable to the sensors. Transparent obstacles are particularly challenging for...the probability that a certain object will be found in a certain space (e.g., the probability that coffee is found in the kitchen). While searching...with which it finds such objects, to find abnormal situations (e.g., if someone is actively hiding all the coffee from the CoBots). One of the most
Multi-center Airborne Coherent Atmospheric Wind Sensor (MACAWS)
NASA Technical Reports Server (NTRS)
Rhothermel, Jeffry; Jones, W. D.; Dunkin, J. A.; Mccaul, E. W., Jr.
1993-01-01
This effort involves development of a calibrated, pulsed coherent CO2 Doppler lidar, followed by a carefully-planned and -executed program of multi-dimensional wind velocity and aerosol backscatter measurements from the NASA DC-8 research aircraft. The lidar, designated as the Multi-center Airborne Coherent Atmospheric Wind Sensor (MACAWS), will be applicable to two research areas. First, MACAWS will enable specialized measurements of atmospheric dynamical processes in the planetary boundary layer and free troposphere in geographic locations and over scales of motion not routinely or easily accessible to conventional sensors. The proposed observations will contribute fundamentally to a greater understanding of the role of the mesoscale, helping to improve predictive capabilities for mesoscale phenomena and to provide insights into improving model parameterizations of sub-grid scale processes within large-scale circulation models. As such, it has the potential to contribute uniquely to major, multi-institutional field programs planned for the mid 1990's. Second, MACAWS measurements can be used to reduce the degree of uncertainty in performance assessments and algorithm development for NASA's prospective Laser Atmospheric Wind Sounder (LAWS), which has no space-based instrument heritage. Ground-based lidar measurements alone are insufficient to address all of the key issues. To minimize costs, MACAWS is being developed cooperatively by the lidar remote sensing groups of the Jet Propulsion Laboratory, NOAA Wave Propagation Laboratory, and MSFC using existing lidar hardware and manpower resources. Several lidar components have already been exercised in previous airborne lidar programs (for example, MSFC Airborne Doppler Lidar System (ADLS) used in 1981,4 Severe Storms Wind Measurement Program; JPL Airborne Backscatter Lidar Experiment (ABLE) used in 1989,90 Global Backscatter Experiment Survey Missions). MSFC has been given responsibility for directing the overall program of instrument development and scientific measurement. The focus of current research and plans for next year are presented.
Ohnishi, Mutsuko; Kato, Shigeko; Razzaque, M. Shawkat
2013-01-01
Objective The adverse effects of metabolic disorders in obesity have been extensively studied; however, the pathologic effects of hyperphosphatemia or phosphate toxicity in obesity have not been studied in similar depth and detail, chiefly because such an association is thought to be uncommon. Studies have established that the incidence of obesity-associated nephropathy is increasing. Because hyperphosphatemia is a major consequence of renal impairment, this study determines the in vivo effects of hyperphosphatemia in obesity. Methods and results We genetically induced hyperphosphatemia in leptin-deficient obese (ob/ob) mice by generating ob/ob and klotho double knockout [ob/ob-klotho−/−] mice. As a control, we made ob/ob mice with hypophosphatemia by generating ob/ob and 1-alpha hydroxylase double knockout [ob/ob-1α(OH)ase−/−] mice. Compared to the wild-type mice, all three obese background mice, namely ob/ob, ob/ob-klotho−/−, and ob/ob-1α(OH)ase−/− mice developed hypercholesterolemia. In addition, the hyperphosphatemic, ob/ob-klotho−/− genetic background induced generalized tissue atrophy and widespread soft-tissue and vascular calcifications, which led to a shorter lifespan; no such changes were observed in the hypophosphatemic, ob/ob-1α(OH)ase−/− mice. Significantly, in contrast to the reduced survival of the ob/ob-klotho−/− mice, lowering serum phosphate levels in ob/ob-1α(OH)ase−/− mice showed no such compromised survival, despite both mice being hypercholesterolemic. Conclusion These genetic manipulation studies suggest phosphate toxicity is an important risk factor in obesity that can adversely affect survival. PMID:22037453
Ohnishi, Mutsuko; Kato, Shigeko; Razzaque, M Shawkat
2011-11-25
The adverse effects of metabolic disorders in obesity have been extensively studied; however, the pathologic effects of hyperphosphatemia or phosphate toxicity in obesity have not been studied in similar depth and detail, chiefly because such an association is thought to be uncommon. Studies have established that the incidence of obesity-associated nephropathy is increasing. Because hyperphosphatemia is a major consequence of renal impairment, this study determines the in vivo effects of hyperphosphatemia in obesity. We genetically induced hyperphosphatemia in leptin-deficient obese (ob/ob) mice by generating ob/ob and klotho double knockout [ob/ob-klotho(-/-)] mice. As a control, we made ob/ob mice with hypophosphatemia by generating ob/ob and 1-alpha hydroxylase double knockout [ob/ob-1α(OH)ase(-/-)] mice. Compared to the wild-type mice, all three obese background mice, namely ob/ob, ob/ob-klotho(-/-), and ob/ob-1α(OH)ase(-/-) mice developed hypercholesterolemia. In addition, the hyperphosphatemic, ob/ob-klotho(-/-) genetic background induced generalized tissue atrophy and widespread soft-tissue and vascular calcifications, which led to a shorter lifespan; no such changes were observed in the hypophosphatemic, ob/ob-1α(OH)ase(-/-) mice. Significantly, in contrast to the reduced survival of the ob/ob-klotho(-/-) mice, lowering serum phosphate levels in ob/ob-1α(OH)ase(-/-) mice showed no such compromised survival, despite both mice being hypercholesterolemic. These genetic manipulation studies suggest phosphate toxicity is an important risk factor in obesity that can adversely affect survival. Copyright © 2011 Elsevier Inc. All rights reserved.
Kloock, Joachim P.; Mourzina, Youlia G.; Ermolenko, Yuri; Doll, Theodor; Schubert, Jürgen; Schöning, Michael J.
2004-01-01
Chalcogenide glasses offer an excellent “challenge” for their use and implementation in sensor arrays due to their good sensor-specific advantages in comparison to their crystalline counterparts. This paper will give an introduction on the preparation of chalcogenide glasses in the thin-film state. First, single microsensors have been prepared with the methods of semiconductor technology. In a next step, three microsensors are implemented onto one single silicon substrate to an “one chip” sensor array. Different ionselective chalcogenide glass membranes (PbSAgIAs2S3, CdSAgIAs2S3, CuAgAsSeTe and TlAgAsIS) were prepared by means of the pulsed laser deposition (PLD) process. The different sensor membranes and structures have been physically characterized by means of Rutherford backscattering spectrometry, scanning electron microscopy and video microscopy. The electrochemical behavior has been investigated by potentiometric measurements.
Sáinz, Neira; Rodríguez, Amaia; Catalán, Victoria; Becerril, Sara; Ramírez, Beatriz; Lancha, Andoni; Burgos-Ramos, Emma; Gómez-Ambrosi, Javier; Frühbeck, Gema
2012-01-01
Leptin improves insulin sensitivity in skeletal muscle. Our goal was to determine whether proteins controlling GLUT4 traffic are altered by leptin deficiency and in vivo leptin administration in skeletal muscle of wild type and ob/ob mice. Leptin-deficient ob/ob mice were divided in three groups: control, leptin-treated (1 mg/kg/d) and leptin pair-fed ob/ob mice. Microarray analysis revealed that 1,546 and 1,127 genes were regulated by leptin deficiency and leptin treatment, respectively. Among these, we identified 24 genes involved in intracellular vesicle-mediated transport in ob/ob mice. TBC1 domain family, member 1 (Tbc1d1), a negative regulator of GLUT4 translocation, was up-regulated (P = 0.001) in ob/ob mice as compared to wild types. Importantly, leptin treatment reduced the transcript levels of Tbc1d1 (P<0.001) and Tbc1d4 (P = 0.004) in the leptin-treated ob/ob as compared to pair-fed ob/ob animals. In addition, phosphorylation levels of TBC1D1 and TBC1D4 were enhanced in leptin-treated ob/ob as compared to control ob/ob (P = 0.015 and P = 0.023, respectively) and pair-fed ob/ob (P = 0.036 and P = 0.034, respectively) mice. Despite similar GLUT4 protein expression in wild type and ob/ob groups a different immunolocalization of this protein was evidenced in muscle sections. Leptin treatment increased GLUT4 immunoreactivity in gastrocnemius and extensor digitorum longus sections of leptin-treated ob/ob mice. Moreover, GLUT4 protein detected in immunoprecipitates from TBC1D4 was reduced by leptin replacement compared to control ob/ob (P = 0.013) and pair-fed ob/ob (P = 0.037) mice. Our findings suggest that leptin enhances the intracellular GLUT4 transport in skeletal muscle of ob/ob animals by reducing the expression and activity of the negative regulators of GLUT4 traffic TBC1D1 and TBC1D4. PMID:22253718
NASA Astrophysics Data System (ADS)
Glennon, John J.; Nichols, Terry; Gatt, Phillip; Baynard, Tahllee; Marquardt, John H.; Vanderbeek, Richard G.
2009-05-01
The weaponization and dissemination of biological warfare agents (BWA) constitute a high threat to civilians and military personnel. An aerosol release, disseminated from a single point, can directly affect large areas and many people in a short time. Because of this threat real-time standoff detection of BWAs is a key requirement for national and military security. BWAs are a general class of material that can refer to spores, bacteria, toxins, or viruses. These bioaerosols have a tremendous size, shape, and chemical diversity that, at present, are not well characterized [1]. Lockheed Martin Coherent Technologies (LMCT) has developed a standoff lidar sensor with high sensitivity and robust discrimination capabilities with a size and ruggedness that is appropriate for military use. This technology utilizes multiwavelength backscatter polarization diversity to discriminate between biological threats and naturally occurring interferents such as dust, smoke, and pollen. The optical design and hardware selection of the system has been driven by performance modeling leading to an understanding of measured system sensitivity. Here we briefly discuss the challenges of standoff bioaerosol discrimination and the approach used by LMCT to overcome these challenges. We review the radiometric calculations involved in modeling direct-detection of a distributed aerosol target and methods for accurately estimating wavelength dependent plume backscatter coefficients. Key model parameters and their validation are discussed and outlined. Metrics for sensor sensitivity are defined, modeled, and compared directly to data taken at Dugway Proving Ground, UT in 2008. Sensor sensitivity is modeled to predict performance changes between day and night operation and in various challenging environmental conditions.
Data documentation for the 1981 summer vegetation experiment. [Kansas River floodplain
NASA Technical Reports Server (NTRS)
Ulaby, F. T. (Principal Investigator); Brisco, B.; Allen, C.
1982-01-01
The mobile agricultural radar sensor was used to collect data from 31 fields in the floodplain of the Kansas River east of Lawrence, Kansas during the summer of 1981. Corn, soybeans, and wheat crops were observed from May 1 to November 11. Radar backscattering measurements were acquired at 10.2 GHz for VV and VH polarizations at 50 deg incidence angles for all fields and at 30 deg, 40 deg, 50 deg, 60 deg, and 70 deg for nine of the 31 fields. Target parameters describing the vegatation and soil characteristics, such as plant moisture, plant height, soil moisture, etc., were also measured. The methodology, radar backscatter data and associated ground-truth data obtained during this experiment are documented.
NASA Technical Reports Server (NTRS)
Grant, W. B.; Hinkley, E. D.
1984-01-01
Remote sensor uses laser radiation backscattered from natural targets. He/Ne Laser System for remote scanning of Methane leaks employs topographic target to scatter light to receiver near laser transmitter. Apparatus powered by 1.5kW generator transported to field sites and pointed at suspected methane leaks. Used for remote detection of natural-gas leaks and locating methane emissions in landfill sites.
2009-09-30
shelled animals using multi-frequency, in situ measurements of backscatter from pteropods .” In review. RESULTS 2008 Field Effort Numerous...using light sensors developed during the 2007 field season . 3 0 50 100 150 200 250 300 2000 2100 2200 2300 0000 0100 Local time (h) D ep th (m ) 0
Genetically obese (ob/ob) mice are resistant to the lethal effects of thioacetamide hepatotoxicity
DOE Office of Scientific and Technical Information (OSTI.GOV)
Won, Young-Suk; Song, Ji-Won; Lim, Jong-Hwan
Obesity increases the risk of chronic liver diseases, including viral hepatitis, alcohol-induced liver disease, and non-alcoholic steatohepatitis. In this study, we investigated the effects of obesity in acute hepatic failure using a murine model of thioacetamide (TA)-induced liver injury. Genetically obese ob/ob mice, together with non-obese ob/+ littermates, were subjected to a single intraperitoneal injection of TA, and examined for signs of hepatic injury. ob/ob mice showed a significantly higher survival rate, lower levels of serum alanine aminotransferase and aspartate aminotransferase, and less hepatic necrosis and apoptosis, compared with ob/+ mice. In addition, ob/ob mice exhibited significantly lower levels ofmore » malondialdehyde and significantly higher levels of glutathione and antioxidant enzyme activities compared with their ob/+ counterparts. Bioactivation analyses revealed reduced plasma clearance of TA and covalent binding of [{sup 14}C]TA to liver macromolecules in ob/ob mice. Together, these data demonstrate that genetically obese mice are resistant to TA-induced acute liver injury through diminished bioactivation of TA and antioxidant effects. - Highlights: • ob/ob mice are resistant to lethal doses of thioacetamide, compared to ob/+ mice. • ob/ob mice show reduced oxidative stress and enhanced antioxidant enzyme activity. • ob/ob mice exhibit diminished bioactivation of thioacetamide.« less
Integrated System for Monitoring and Prevention in Obstetrics-Gynaecology.
Robu, Andreea; Gauca, Bianca; Crisan-Vida, Mihaela; Stoicu-Tivadar, Lăcrămioara
2016-01-01
A better monitoring of pregnant women, mainly during the third trimester of pregnancy and an easy communication between physician and patients are very important for the prevention and good health of baby and mother. The paper presents an integrated system as support for the Obstetrics - Gynaecology domain consisting in two modules: a mobile application, ObGynCare, dedicated to the pregnant women and a new component of the Obstetrics-Gynaecology Department Information System dedicated to the physicians for a better monitoring of the pregnant women. The mobile application informs the pregnant women about their status, permits them to introduce glycaemia and weight values and has as option pulse and blood pressure acquisition from a smart sensor and provides results in a graphic format. It also provides support for easy patient-doctor communication related to any health problems. ObGyn Care offers nutrition recommendations and gives the pregnant women the possibility to enter a social space of common interests using social networks (Facebook) to exchange useful and practical information. Data collected from patients and from sensor are stored on the cloud and the physician may access the information and analyse it. The extended module of the Obstetrics-Gynaecology Department Information System already developed supports the physicians to visualize weekly, monthly, or on a trimester, the patient data and to discuss with her through the chat module. The mobile application is in test by pregnant women and medical personnel.
Obesity induced alterations in redox homeostasis and oxidative stress are present from an early age
Lechuga-Sancho, Alfonso M.; Gallego-Andujar, David; Ruiz-Ocaña, Pablo; Visiedo, Francisco M.; Saez-Benito, Ana; Schwarz, Mónica; Segundo, Carmen
2018-01-01
Objectives Oxidative stress and inflammation have been postulated as underlying mechanisms for the development of obesity-related insulin resistance. This association however, remains elusive especially in childhood. We sought to investigate this relation by measuring oxidative stress and antioxidant response biomarkers, before and during an oral glucose tolerance test (OGTT), in different biological samples from obese children. Subjects 24 children were recruited for the study, (18 obese and 6 controls). After OGTT, the obese group was subdivided in two, according to whether or not carbohydrate metabolic impairment (Ob.IR+, Ob.IR-; respectively) was found. Different biomarkers were analyzed after fasting (T = 0) and during an OGTT (T = 60 and 120 min). Lipoperoxides were measured in plasma, erythrocytes, and urine; while advanced glycation end products were determined in plasma, and redox status (GSH/GSSG ratio) in erythrocytes. Results We found marked differences in the characterization of the oxidative status in urine and erythrocytes, and in the dynamics of the antioxidant response during OGTT. Specifically, Ob.IR+ children show increased oxidative stress, deficient antioxidant response and a significant imbalance in redox status, in comparison to controls and Ob.IR- children. Conclusion Obese children with insulin resistance show increased levels of oxidative stress biomarkers, and a stunted antioxidant response to an OGTT leading to increased oxidative stress after a single glucose load, as detected in erythrocytes, but not in plasma. We propose erythrocytes as sensors of early and acute changes in oxidative stress associated with insulin resistance in childhood obesity. This is a pilot study, performed with a limited sample size, so data should be interpreted with caution until reproduced. PMID:29370267
Wachowiak, Matt; Economo, Michael N.; Díaz-Quesada, Marta; Brunert, Daniela; Wesson, Daniel W.; White, John. A.; Rothermel, Markus
2013-01-01
Understanding central processing requires precise monitoring of neural activity across populations of identified neurons in the intact brain. Here we used recently-optimized variants of the genetically-encoded calcium sensor GCaMP (GCaMP3 and GCaMPG5G) to image activity among genetically- and anatomically-defined neuronal populations in the olfactory bulb (OB), including two types of GABA-ergic interneurons (periglomerular (PG) and short axon (SA) cells) and OB output neurons (mitral/tufted (MT) cells) projecting to piriform cortex. We first established that changes in neuronal spiking can be accurately related to GCaMP fluorescence changes via a simple quantitative relationship over a large dynamic range. We next used in vivo two-photon imaging from individual neurons and epifluorescence signals reflecting population-level activity to investigate the spatiotemporal representation of odorants across these neuron types in anesthetized and awake mice. Under anesthesia, individual PG and SA cells showed temporally simple responses and little spontaneous activity, while MT cells were spontaneously active and showed diverse temporal responses. At the population level, response patterns of PG, SA and MT cells were surprisingly similar to those imaged from sensory inputs, with shared odorant-specific topography across the dorsal OB and inhalation-coupled temporal dynamics. During wakefulness, PG and SA cell responses increased in magnitude but remained temporally simple while those of MT cells changed to complex spatiotemporal patterns reflecting restricted excitation and widespread inhibition. These results point to multiple circuit elements with distinct roles in transforming odor representations in the OB and provide a framework for further dissecting early olfactory processing using optical and genetic tools. PMID:23516293
Novel laser Doppler flowmeter for pulpal blood flow measurements
NASA Astrophysics Data System (ADS)
Zang, De Yu; Millerd, James E.; Wilder-Smith, Petra B. B.; Arrastia-Jitosho, Anna-Marie A.
1996-04-01
We have proposed and experimentally demonstrated a new configuration of laser Doppler flowmetry for dental pulpal blood flow measurements. To date, the vitality of a tooth can be determined only by subjective thermal or electric tests, which are of questionable reliability and may induced pain in patient. Non-invasive techniques for determining pulpal vascular reactions to injury, treatment, and medication are in great demand. The laser Doppler flowmetry technique is non-invasive; however, clinical studies have shown that when used to measure pulpal blood flow the conventional back-scattering Doppler method suffers from low signal-to-noise ratio (SNR) and unreliable flux readings rendering it impossible to calibrate. A simplified theoretical model indicates that by using a forward scattered geometry the detected signal has a much higher SNR and can be calibrated. The forward scattered signal is readily detectable due to the fact that teeth are relatively thin organs with moderate optical loss. A preliminary experiment comparing forward scattered detection with conventional back- scattered detection was carried out using an extracted human molar. The results validated the findings of the simple theoretical model and clearly showed the utility of the forward scattering geometry. The back-scattering method had readings that fluctuated by as much as 187% in response to small changes in sensor position relative to the tooth. The forward scattered method had consistent readings (within 10%) that were independent of the sensor position, a signal-to-noise ratio that was at least 5.6 times higher than the back-scattering method, and a linear response to flow rate.
Öztürk, Mehmet; Work, Paul A.
2016-01-01
A data set was acquired on a shallow mudflat in south San Francisco Bay that featured simultaneous, co-located optical and acoustic sensors for subsequent estimation of suspended sediment concentrations (SSC). The optical turbidity sensor output was converted to SSC via an empirical relation derived at a nearby site using bottle sample estimates of SSC. The acoustic data was obtained using an acoustic Doppler velocimeter. Backscatter and noise were combined to develop another empirical relation between the optical estimates of SSC and the relative backscatter from the acoustic velocimeter. The optical and acoustic approaches both reproduced similar general trends in the data and have merit. Some seasonal variation in the dataset was evident, with the two methods differing by greater or lesser amounts depending on which portion of the record was examined. It is hypothesized that this is the result of flocculation, affecting the two signals by different degrees, and that the significance or mechanism of the flocculation has some seasonal variability. In the earlier portion of the record (March), there is a clear difference that appears in the acoustic approach between ebb and flood periods, and this is not evident later in the record (May). The acoustic method has promise but it appears that characteristics of flocs that form and break apart may need to be accounted for to improve the power of the method. This may also be true of the optical method: both methods involve assuming that the sediment characteristics (size, size distribution, and shape) are constant.
Ozturk, Mehmet; Work, Paul A.
2016-01-01
A data set was acquired on a shallow mudflat in south San Francisco Bay that featured simultaneous, co-located optical and acoustic sensors for subsequent estimation of suspended sediment concentrations (SSC). The optical turbidity sensor output was converted to SSC via an empirical relation derived at a nearby site using bottle sample estimates of SSC. The acoustic data was obtained using an acoustic Doppler velocimeter. Backscatter and noise were combined to develop another empirical relation between the optical estimates of SSC and the relative backscatter from the acoustic velocimeter. The optical and acoustic approaches both reproduced similar general trends in the data and have merit. Some seasonal variation in the dataset was evident, with the two methods differing by greater or lesser amounts depending on which portion of the record was examined. It is hypothesized that this is the result of flocculation, affecting the two signals by different degrees, and that the significance or mechanism of the flocculation has some seasonal variability. In the earlier portion of the record (March), there is a clear difference that appears in the acoustic approach between ebb and flood periods, and this is not evident later in the record (May). The acoustic method has promise but it appears that characteristics of flocs that form and break apart may need to be accounted for to improve the power of the method. This may also be true of the optical method: both methods involve assuming that the sediment characteristics (size, size distribution, and shape) are constant
NASA Technical Reports Server (NTRS)
Lee, Zhong-Ping; Carder, Kendall L.
2001-01-01
A multi-band analytical (MBA) algorithm is developed to retrieve absorption and backscattering coefficients for optically deep waters, which can be applied to data from past and current satellite sensors, as well as data from hyperspectral sensors. This MBA algorithm applies a remote-sensing reflectance model derived from the Radiative Transfer Equation, and values of absorption and backscattering coefficients are analytically calculated from values of remote-sensing reflectance. There are only limited empirical relationships involved in the algorithm, which implies that this MBA algorithm could be applied to a wide dynamic range of waters. Applying the algorithm to a simulated non-"Case 1" data set, which has no relation to the development of the algorithm, the percentage error for the total absorption coefficient at 440 nm a (sub 440) is approximately 12% for a range of 0.012 - 2.1 per meter (approximately 6% for a (sub 440) less than approximately 0.3 per meter), while a traditional band-ratio approach returns a percentage error of approximately 30%. Applying it to a field data set ranging from 0.025 to 2.0 per meter, the result for a (sub 440) is very close to that using a full spectrum optimization technique (9.6% difference). Compared to the optimization approach, the MBA algorithm cuts the computation time dramatically with only a small sacrifice in accuracy, making it suitable for processing large data sets such as satellite images. Significant improvements over empirical algorithms have also been achieved in retrieving the optical properties of optically deep waters.
Ma, Xiaojun; Lin, Yuezhen; Lin, Ligen; Qin, Guijun; Pereira, Fred A; Haymond, Morey W; Butte, Nancy F; Sun, Yuxiang
2012-08-01
The orexigenic hormone ghrelin is important in diabetes because it has an inhibitory effect on insulin secretion. Ghrelin ablation in leptin-deficient ob/ob (Ghrelin(-/-):ob/ob) mice increases insulin secretion and improves hyperglycemia. The physiologically relevant ghrelin receptor is the growth hormone secretagogue receptor (GHS-R), and GHS-R antagonists are thought to be an effective strategy for treating diabetes. However, since some of ghrelin's effects are independent of GHS-R, we have utilized genetic approaches to determine whether ghrelin's effect on insulin secretion is mediated through GHS-R and whether GHS-R antagonism indeed inhibits insulin secretion. We investigated the effects of GHS-R on glucose homeostasis in Ghsr-ablated ob/ob mice (Ghsr(-/-):ob/ob). Ghsr ablation did not rescue the hyperphagia, obesity, or insulin resistance of ob/ob mice. Surprisingly, Ghsr ablation worsened the hyperglycemia, decreased insulin, and impaired glucose tolerance. Consistently, Ghsr ablation in ob/ob mice upregulated negative β-cell regulators (such as UCP-2, SREBP-1c, ChREBP, and MIF-1) and downregulated positive β-cell regulators (such as HIF-1α, FGF-21, and PDX-1) in whole pancreas; this suggests that Ghsr ablation impairs pancreatic β-cell function in leptin deficiency. Of note, Ghsr ablation in ob/ob mice did not affect the islet size; the average islet size of Ghsr(-/-):ob/ob mice is similar to that of ob/ob mice. In summary, because Ghsr ablation in leptin deficiency impairs insulin secretion and worsens hyperglycemia, this suggests that GHS-R antagonists may actually aggravate diabetes under certain conditions. The paradoxical effects of ghrelin ablation and Ghsr ablation in ob/ob mice highlight the complexity of the ghrelin-signaling pathway.
Emerging Needs for Pervasive Passive Wireless Sensor Networks on Aerospace Vehicles
NASA Technical Reports Server (NTRS)
Wilson, William C.; Juarez, Peter D.
2014-01-01
NASA is investigating passive wireless sensor technology to reduce instrumentation mass and volume in ground testing, air flight, and space exploration applications. Vehicle health monitoring systems (VHMS) are desired on all aerospace programs to ensure the safety of the crew and the vehicles. Pervasive passive wireless sensor networks facilitate VHMS on aerospace vehicles. Future wireless sensor networks on board aerospace vehicles will be heterogeneous and will require active and passive network systems. Since much has been published on active wireless sensor networks, this work will focus on the need for passive wireless sensor networks on aerospace vehicles. Several passive wireless technologies such as microelectromechanical systems MEMS, SAW, backscatter, and chipless RFID techniques, have all shown potential to meet the pervasive sensing needs for aerospace VHMS applications. A SAW VHMS application will be presented. In addition, application areas including ground testing, hypersonic aircraft and spacecraft will be explored along with some of the harsh environments found in aerospace applications.
Phase difference statistics related to sensor and forest parameters
NASA Technical Reports Server (NTRS)
Lopes, A.; Mougin, E.; Beaudoin, A.; Goze, S.; Nezry, E.; Touzi, R.; Karam, M. A.; Fung, A. K.
1992-01-01
The information content of ordinary synthetic aperture radar (SAR) data is principally contained in the radiometric polarization channels, i.e., the four Ihh, Ivv, Ihv and Ivh backscattered intensities. In the case of clutter, polarimetric information is given by the four complex degrees of coherence, from which the mean polarization phase differences (PPD), correlation coefficients or degrees of polarization can be deduced. For radiometric features, the polarimetric parameters are corrupted by multiplicative speckle noise and by some sensor effects. The PPD distribution is related to the sensor, speckle and terrain properties. Experimental results are given for the variation of the terrain hh/vv mean phase difference and magnitude of the degree of coherence observed on bare soil and on different pine forest stands.
Satellite stratospheric aerosol measurement validation
NASA Technical Reports Server (NTRS)
Russell, P. B.; Mccormick, M. P.
1984-01-01
The validity of the stratospheric aerosol measurements made by the satellite sensors SAM II and SAGE was tested by comparing their results with each other and with results obtained by other techniques (lider, dustsonde, filter, and impactor). The latter type of comparison required the development of special techniques that convert the quantity measured by the correlative sensor (e.g. particle backscatter, number, or mass) to that measured by the satellite sensor (extinction) and quantitatively estimate the uncertainty in the conversion process. The results of both types of comparisons show agreement within the measurement and conversion uncertainties. Moreover, the satellite uncertainty is small compared to aerosol natural variability (caused by seasonal changes, volcanoes, sudden warmings, and vortex structure). It was concluded that the satellite measurements are valid.
Wide-beam sensors for controlling dual-delay systems
NASA Astrophysics Data System (ADS)
Edwards, J. B.; Twemlow, J. K.
1982-09-01
A class of dual delay feedback systems of open loop transfer function G(s) = k exp(-Xs)/l - exp(-Ws) is shown to be unstable if ratio X/W is noninteger. By means of z-transform techniques it is shown that, by using a feedback transducer that senses over a substantial distance either side of its central axis, closed-loop stability may be restored. Such transducers, termed widebeam sensors, include transmission, backscatter and natural radiation types as well as electromechanical conveyor belt weighers. Designing transducers for very narrow beams may not be desirable from the overall system viewpoint.
Wendel, Angela A.; Li, Lei O.; Li, Yue; Cline, Gary W.; Shulman, Gerald I.; Coleman, Rosalind A.
2010-01-01
OBJECTIVE Hepatic steatosis is strongly associated with insulin resistance, but a causal role has not been established. In ob/ob mice, sterol regulatory element binding protein 1 (SREBP1) mediates the induction of steatosis by upregulating target genes, including glycerol-3-phosphate acyltransferase-1 (Gpat1), which catalyzes the first and committed step in the pathway of glycerolipid synthesis. We asked whether ob/ob mice lacking Gpat1 would have reduced hepatic steatosis and improved insulin sensitivity. RESEARCH DESIGN AND METHODS Hepatic lipids, insulin sensitivity, and hepatic insulin signaling were compared in lean (Lep+/?), lean-Gpat1−/−, ob/ob (Lepob/ob), and ob/ob-Gpat1−/− mice. RESULTS Compared with ob/ob mice, the lack of Gpat1 in ob/ob mice reduced hepatic triacylglycerol (TAG) and diacylglycerol (DAG) content 59 and 74%, respectively, but increased acyl-CoA levels. Despite the reduction in hepatic lipids, fasting glucose and insulin concentrations did not improve, and insulin tolerance remained impaired. In both ob/ob and ob/ob-Gpat1−/− mice, insulin resistance was accompanied by elevated hepatic protein kinase C-ε activation and blunted insulin-stimulated Akt activation. CONCLUSIONS These results suggest that decreasing hepatic steatosis alone does not improve insulin resistance, and that factors other than increased hepatic DAG and TAG contribute to hepatic insulin resistance in this genetically obese model. They also show that the SREBP1-mediated induction of hepatic steatosis in ob/ob mice requires Gpat1. PMID:20200319
Effect of leptin administration on myelination in ob/ob mouse cerebrum after birth.
Hashimoto, Ryuju; Matsumoto, Akihiro; Udagawa, Jun; Hioki, Kyoji; Otani, Hiroki
2013-01-09
Brain weight and size are known to be reduced in adult leptin-deficient Lep/Lep (OB) mice when compared with the wild-type (+/+) mice (C57BL/6: B6). We here analyzed leptin's effects on myelination by examining morphometrically the myelin sheath (MS) in the cerebrum of postnatal day (P) 14 and P28 OB that had received leptin 1 nmol/capita/day from P7 to P14 or P28 (OB+lep), in comparison with OB and B6. We examined myelin basic protein (MBP) mRNA levels and the differentiation of oligodendrocytes by comparing the number of oligodendrocyte precursor cells (OPCs) and the mature oligodendrocytes in the cerebrum between OB, OB+lep, and B6 on P14 and P28. MBP-mRNA expression was lower in OB than in B6 on P14 and P28. On P14, it was higher in OB+lep than in OB but was still lower than in B6, whereas on P28 it was even higher in OB+lep than in B6. On P28, the radii of myelinated axons were larger in OB than in B6 and OB+lep. The MS on P28 was significantly thinner in OB than in B6, but there was no significant difference between OB and OB+lep. There were significantly fewer mature oligodendrocytes in OB and OB+lep than in B6 on P28, whereas on P14 there were significantly fewer OPCs in OB and OB+lep than in B6. Our results suggested that leptin regulates the myelination of oligodendrocytes and that the replenishment of leptin in OB recovered myelination but did not affect the differentiation of OPCs from P7 to P28.
Role of estrogen receptor signaling in skeletal response to leptin in female ob/ob mice.
Turner, Russell T; Philbrick, Kenneth A; Kuah, Amida F; Branscum, Adam J; Iwaniec, Urszula T
2017-06-01
Leptin, critical in regulation of energy metabolism, is also important for normal bone growth, maturation and turnover. Compared to wild type (WT) mice, bone mass is lower in leptin-deficient ob/ob mice. Osteopenia in growing ob/ob mice is due to decreased bone accrual, and is associated with reduced longitudinal bone growth, impaired cancellous bone maturation and increased marrow adipose tissue (MAT). However, leptin deficiency also results in gonadal dysfunction, disrupting production of gonadal hormones which regulate bone growth and turnover. The present study evaluated the role of increased estrogen in mediating the effects of leptin on bone in ob/ob mice. Three-month-old female ob/ob mice were randomized into one of the 3 groups: (1) ob/ob + vehicle (veh), (2) ob/ob + leptin (leptin) or (3) ob/ob + leptin and the potent estrogen receptor antagonist ICI 182,780 (leptin + ICI). Age-matched WT mice received vehicle. Leptin (40 µg/mouse, daily) and ICI (10 µg/mouse, 2×/week) were administered by subcutaneous injection for 1 month and bone analyzed by X-ray absorptiometry, microcomputed tomography and static and dynamic histomorphometry. Uterine weight did not differ between ob/ob mice and ob/ob mice receiving leptin + ICI, indicating that ICI successfully blocked the uterine response to leptin-induced increases in estrogen levels. Compared to leptin-treated ob/ob mice, ob/ob mice receiving leptin + ICI had lower uterine weight; did not differ in weight loss, MAT or bone formation rate; and had higher longitudinal bone growth rate and cancellous bone volume fraction. We conclude that increased estrogen signaling following leptin treatment is dispensable for the positive actions of leptin on bone and may attenuate leptin-induced bone growth. © 2017 Society for Endocrinology.
Acoustic backscatter models of fish: Gradual or punctuated evolution
NASA Astrophysics Data System (ADS)
Horne, John K.
2004-05-01
Sound-scattering characteristics of aquatic organisms are routinely investigated using theoretical and numerical models. Development of the inverse approach by van Holliday and colleagues in the 1970s catalyzed the development and validation of backscatter models for fish and zooplankton. As the understanding of biological scattering properties increased, so did the number and computational sophistication of backscatter models. The complexity of data used to represent modeled organisms has also evolved in parallel to model development. Simple geometric shapes representing body components or the whole organism have been replaced by anatomically accurate representations derived from imaging sensors such as computer-aided tomography (CAT) scans. In contrast, Medwin and Clay (1998) recommend that fish and zooplankton should be described by simple theories and models, without acoustically superfluous extensions. Since van Holliday's early work, how has data and computational complexity influenced accuracy and precision of model predictions? How has the understanding of aquatic organism scattering properties increased? Significant steps in the history of model development will be identified and changes in model results will be characterized and compared. [Work supported by ONR and the Alaska Fisheries Science Center.
Specific absorption and backscatter coefficient signatures in southeastern Atlantic coastal waters
NASA Astrophysics Data System (ADS)
Bostater, Charles R., Jr.
1998-12-01
Measurements of natural water samples in the field and laboratory of hyperspectral signatures of total absorption and reflectance were obtained using long pathlength absorption systems (50 cm pathlength). Water was sampled in Indian River Lagoon, Banana River and Port Canaveral, Florida. Stations were also occupied in near coastal waters out to the edge of the Gulf Stream in the vicinity of Kennedy Space Center, Florida and estuarine waters along Port Royal Sound and along the Beaufort River tidal area in South Carolina. The measurements were utilized to calculate natural water specific absorption, total backscatter and specific backscatter optical signatures. The resulting optical cross section signatures suggest different models are needed for the different water types and that the common linear model may only appropriate for coastal and oceanic water types. Mean particle size estimates based on the optical cross section, suggest as expected, that particle size of oceanic particles are smaller than more turbid water types. The data discussed and presented are necessary for remote sensing applications of sensors as well as for development and inversion of remote sensing algorithms.
2009-01-01
1008.3 r <•-• ADOR/Director NCST E. R. Franchi , 7000 Public Affairs (Unclassified/ Unlimited Only), Code 703Q 4 ’ iJL:,. iUn’i i’-"Vt... global ocean color sensors (e.g., MODIS). Also, this resolution roughly matches the swath of MicroSAS radiometric measurements in the visible range
1988-05-01
interests are centered on signal processing and the physics of underwater acoustic propagation, ambient noise, and reverberation. Mr. Rojas is a member...Airborne underwater acoustics Bottom-limited acoustics Arctic underwater acoustics Propagation Noise Ambient noise measurements and modeling Spectral...Multispectral image correlation Space sensor and mission analysis CROWN" STALK Time, ms (100 ms after impactO Time-history of the . radar backscatter from a
Tseng, Hsiu-Ting; Park, Young Joo; Lee, Yoon Kwang; Moore, David D
2015-05-08
Small heterodimer partner (SHP, NR0B2) is involved in diverse metabolic pathways, including hepatic bile acid, lipid and glucose homeostasis, and has been implicated in effects on the peroxisome proliferator-activated receptor γ (PPARγ), a master regulator of adipogenesis and the receptor for antidiabetic drugs thiazolidinediones (TZDs). In this study, we aim to investigate the role of SHP in TZD response by comparing TZD-treated leptin-deficient (ob/ob) and leptin-, SHP-deficient (ob/ob;Shp(-/-)) double mutant mice. Both ob/ob and double mutant ob/ob;Shp(-/-) mice developed hyperglycemia, insulin resistance, and hyperlipidemia, but hepatic fat accumulation was decreased in the double mutant ob/ob;Shp(-/-) mice. PPARγ2 mRNA levels were markedly lower in ob/ob;Shp(-/-) liver and decreased to a lesser extent in adipose tissue. The TZD troglitazone did not reduce glucose or circulating triglyceride levels in ob/ob;Shp(-/-) mice. Expression of the adipocytokines, such as adiponectin and resistin, was not stimulated by troglitazone treatment. Expression of hepatic lipogenic genes was also reduced in ob/ob;Shp(-/-) mice. Moreover, overexpression of SHP by adenovirus infection increased PPARγ2 mRNA levels in mouse primary hepatocytes. Our results suggest that SHP is required for both antidiabetic and hypolipidemic effects of TZDs in ob/ob mice through regulation of PPARγ expression.
USDA-ARS?s Scientific Manuscript database
The orexigenic hormone ghrelin is important in diabetes because it has an inhibitory effect on insulin secretion. Ghrelin ablation in leptin-deficient ob/ob (Ghrelin(-/-):ob/ob) mice increases insulin secretion and improves hyperglycemia. The physiologically relevant ghrelin receptor is the growth ...
Fiber optic distributed temperature sensor mapping of a jet-mixing flow field
Lomperski, Stephen; Gerardi, Craig; Pointer, William David
2015-03-04
In this paper, we introduce the use of a Rayleigh backscatter-based distributed fiber optic sensor to map the temperature field in air flow for a thermal fatigue application. The experiment involves a pair of air jets at 22 and 70°C discharging from 136 mm hexagonal channels into a 1 × 1 × 1.7 m tank at atmospheric pressure. A 40 m-long, Φ155 µm fiber optic sensor was wound back and forth across the tank midplane to form 16 horizontal measurement sections with a vertical spacing of 51 mm. This configuration generated a 2D temperature map with 2800 data points overmore » a 0.76 × 1.7 m plane. Fiber optic sensor readings were combined with PIV and infrared measurements to relate flow field characteristics to the thermal signature of the tank lid. The paper includes sensor stability data and notes issues encountered using the distributed temperature sensor in a flow field. In conclusion, sensors are sensitive to strain and humidity, and so accuracy relies upon strict control of both.« less
Fiber optic distributed temperature sensor mapping of a jet-mixing flow field
DOE Office of Scientific and Technical Information (OSTI.GOV)
Lomperski, Stephen; Gerardi, Craig; Pointer, William David
In this paper, we introduce the use of a Rayleigh backscatter-based distributed fiber optic sensor to map the temperature field in air flow for a thermal fatigue application. The experiment involves a pair of air jets at 22 and 70°C discharging from 136 mm hexagonal channels into a 1 × 1 × 1.7 m tank at atmospheric pressure. A 40 m-long, Φ155 µm fiber optic sensor was wound back and forth across the tank midplane to form 16 horizontal measurement sections with a vertical spacing of 51 mm. This configuration generated a 2D temperature map with 2800 data points overmore » a 0.76 × 1.7 m plane. Fiber optic sensor readings were combined with PIV and infrared measurements to relate flow field characteristics to the thermal signature of the tank lid. The paper includes sensor stability data and notes issues encountered using the distributed temperature sensor in a flow field. In conclusion, sensors are sensitive to strain and humidity, and so accuracy relies upon strict control of both.« less
Passive Sensors for Long Duration Internet of Things Networks.
Pereira, Felisberto; Correia, Ricardo; Carvalho, Nuno Borges
2017-10-03
In this work, three different concepts are used to develop a fully passive sensor that is capable of measuring different types of data. The sensor was supplied by Wireless Power Transmission (WPT). Communication between the sensor and reader is established by a backscatter, and to ensure minimum energy consumption, low power techniques are used. In a simplistic way, the process starts by the transmission of two different waves by the reader to the sensor, one of which is used in power transmission and the other of which is used to communicate. Once the sensor is powered, the monitoring process starts. From the monitoring state, results from after processing are used to modulate the incoming wave, which is the information that is sent back from the reader to the tag. This new combination of technologies enables the possibility of using sensors without any cables or batteries to operate 340 cm from the reader. The developed prototype measures acceleration and temperature. However, it is scalable. This system enables a new generation of passive Internet of Things (IoT) devices.
Passive Sensors for Long Duration Internet of Things Networks
Correia, Ricardo; Carvalho, Nuno Borges
2017-01-01
In this work, three different concepts are used to develop a fully passive sensor that is capable of measuring different types of data. The sensor was supplied by Wireless Power Transmission (WPT). Communication between the sensor and reader is established by a backscatter, and to ensure minimum energy consumption, low power techniques are used. In a simplistic way, the process starts by the transmission of two different waves by the reader to the sensor, one of which is used in power transmission and the other of which is used to communicate. Once the sensor is powered, the monitoring process starts. From the monitoring state, results from after processing are used to modulate the incoming wave, which is the information that is sent back from the reader to the tag. This new combination of technologies enables the possibility of using sensors without any cables or batteries to operate 340 cm from the reader. The developed prototype measures acceleration and temperature. However, it is scalable. This system enables a new generation of passive Internet of Things (IoT) devices. PMID:28972554
2014-02-01
F. J. Moffat9, and N. R. Walborn2 1 Center for High Angular Resolution Astronomy, Department of Physics and Astronomy, Georgia State University, P.O...Martin Drive, Baltimore, MD 21218, USA; nelan@stsci.edu, walborn@stsci.edu 3 Department of Natural Sciences, University of South Carolina Beaufort, 1 ...Online-only material: figure set 1 . INTRODUCTION Massive stars (10M) play a fundamental role in the evo- lution of the universe, from influencing
A Study of the Vertical Component of Ocean Floor Vibrations in Two Geographical Chokepoints
2017-05-30
Libby Reissued 30 May 2017 with Second Reader’s non -NPS affiliation added to title page. THIS PAGE INTENTIONALLY LEFT BLANK i REPORT DOCUMENTATION...choke points were considered to be a good representation of where these experimental bottom mounted sensors might be located should they be built...calibrated data, and the methodology used to get the calibrated data is discussed in detail. The results showed that one OBS out of the four was highly
Zhou, Da-Peng; Li, Wenhai; Chen, Liang; Bao, Xiaoyi
2013-01-31
A distributed optical fiber sensor with the capability of simultaneously measuring temperature and strain is proposed using a large effective area non-zero dispersion shifted fiber (LEAF) with sub-meter spatial resolution. The Brillouin frequency shift is measured using Brillouin optical time-domain analysis (BOTDA) with differential pulse-width pair technique, while the spectrum shift of the Rayleigh backscatter is measured using optical frequency-domain reflectometry (OFDR). These shifts are the functions of both temperature and strain, and can be used as two independent parameters for the discrimination of temperature and strain. A 92 m measurable range with the spatial resolution of 50 cm is demonstrated experimentally, and accuracies of ±1.2 °C in temperature and ±15 με in strain could be achieved.
Parameter Space of the Columbia River Estuarine Turbidity Maxima
NASA Astrophysics Data System (ADS)
McNeil, C. L.; Shcherbina, A.; Lopez, J.; Karna, T.; Baptista, A. M.; Crump, B. C.; Sanford, T. B.
2016-12-01
We present observations of estuarine turbidity maxima (ETM) in the North Channel of the Columbia River estuary (OR and WA, USA) covering different river discharge and flood tide conditions. Measurements were made using optical backscattering sensors on two REMUS-100 autonomous underwater vehicles (AUVs) during spring 2012, summer 2013, and fall 2012. Although significant short term variability in AUV measured optical backscatter was observed, some clustering of the data occurs around the estuarine regimes defined by a mixing parameter and a freshwater Froude number (Geyer & MacCready [2014]). Similar clustering is observed in long term time series of turbidity from the SATURN observatory. We will use available measurements and numerical model simulations of suspended sediment to further explore the variability of suspended sediment dynamics within a frame work of estuarine parameter space.
Zhou, Tao; Li, Zhaofu; Pan, Jianjun
2018-01-27
This paper focuses on evaluating the ability and contribution of using backscatter intensity, texture, coherence, and color features extracted from Sentinel-1A data for urban land cover classification and comparing different multi-sensor land cover mapping methods to improve classification accuracy. Both Landsat-8 OLI and Hyperion images were also acquired, in combination with Sentinel-1A data, to explore the potential of different multi-sensor urban land cover mapping methods to improve classification accuracy. The classification was performed using a random forest (RF) method. The results showed that the optimal window size of the combination of all texture features was 9 × 9, and the optimal window size was different for each individual texture feature. For the four different feature types, the texture features contributed the most to the classification, followed by the coherence and backscatter intensity features; and the color features had the least impact on the urban land cover classification. Satisfactory classification results can be obtained using only the combination of texture and coherence features, with an overall accuracy up to 91.55% and a kappa coefficient up to 0.8935, respectively. Among all combinations of Sentinel-1A-derived features, the combination of the four features had the best classification result. Multi-sensor urban land cover mapping obtained higher classification accuracy. The combination of Sentinel-1A and Hyperion data achieved higher classification accuracy compared to the combination of Sentinel-1A and Landsat-8 OLI images, with an overall accuracy of up to 99.12% and a kappa coefficient up to 0.9889. When Sentinel-1A data was added to Hyperion images, the overall accuracy and kappa coefficient were increased by 4.01% and 0.0519, respectively.
NASA Astrophysics Data System (ADS)
Bécel, Anne; Laigle, Mireille; Diaz, Jordi; Hirn, Alfred; Flueh, Ernst; Charvis, Philippe
2010-05-01
In the frame of the European Union « THALES WAS RIGHT » and French ANR CATTELL SUBSISMANTI funding, an unprecedented array of 80 OBS, Ocean Bottom Seismometers of Géoazur Nice, INSU/IPGP Paris, IfM-GEOMAR Kiel, AWI Bremerhaven could gathered. They have been deployed for continuous recording over four months on the fore-arc domain of the Lesser Antilles subduction zone offshore Martinique, Dominica, Guadeloupe and Antigua Islands, by scientific cruises of N/O ATALANTE, F/S M. A. MERIAN and N/O ANTEA. One of the aims of this OBS array was the feasibility study of detecting at sea-bottom the seismological part of recently discovered phenomena such as NVT non-volcanic tremors and LP, for Long-Period events. The ability of detecting such transient signals is of importance, since they are possibly related to potential mega-thrust earthquakes and their preparation zone. At the Lesser Antilles subduction zone, the fore-arc domain overlying the seismogenic part of the interplate is located offshore, covered by as much as 4000 m of water. In this case, transient signals can be accessible only from OBS observations. Hence, there is a major difference, in the sense of the instrumental and logistical effort, with the subductions under NW US-Canada and under Central Japan where these signals have been discovered. There, the subduction zones have an emerged fore-arc that has allowed the chance discovery of those phenomena by regular instrument maintained routinely on land. Over 20 of the instruments were BB-OBS, with broadband seismic sensors, possibly the largest such gathering at the time of the experiment among the OBS types. Among those broadband OBS designed or used by different Institutions, there were at least three different seismometer brands and acoustical sensors, as well as different mechanical mounting and technical solutions for coupling them to ground. This did not facilitate data recovery and processing, but on the other hand, as planned by interweaving the different instruments deployments, it provided diverse views, as through different glasses. This ultimately proved valuable to help extract the harder facts from their diverse appearances when seen through different instruments and in different types of sites. After analyzing the data for spurious and instrument-related peculiarities, and possible interpretation pitfalls, it remains that the noise level shows an overwhelming influence of the marine domain due to both its own sources, hydrosphere motions, and to meteorological-climatological actions. As well, the response of the laterally variable fore-arc basin on top of which measurements have to be made is much adverse to quality recording, with respect to seismological observatories on land which can be buried deep into basement rocks. The study of this noise itself may allow us to initiate a discussion of the interactions of the oceanic and atmospheric processes with the Solid Earth. Transients at depth in the subduction zone have been tentatively discussed in terms of its seismogenic evolution. If such transient events would indeed have a component over a very broad spectral range from NVT to LP and ULP events as it has been suggested very recently in Japan (Ide et al., 2008), the conditions and the best observation windows in which they can be best searched for are now documented for ocean bottom recording in the case of the Lesser Antilles subduction zone.
NASA Astrophysics Data System (ADS)
Mahdavi, Sahel; Maghsoudi, Yasser; Amani, Meisam
2017-07-01
Environmental conditions have considerable effects on synthetic aperture radar (SAR) imagery. Therefore, assessing these effects is important for obtaining accurate and reliable results. In this study, three series of RADARSAT-2 SAR images were evaluated. In each of these series, the sensor configuration was fixed, but the environmental conditions differed. The effects of variable environmental conditions were also investigated on co- and cross-polarized backscattering coefficients, Freeman-Durden scattering contributions, and the pedestal height in different classes of a forest area in Ottawa, Ontario. It was observed that the backscattering coefficient of wet snow was up to 2 dB more than that of dry snow. The absence of snow also caused a decrease of up to 3 dB in the surface scattering of ground and up to 5 dB in that of trees. In addition, the backscatter coefficients of ground vegetation, hardwood species, and softwood species were more similar at temperatures below 0°C than those at temperatures above 0°C. Moreover, the pedestal height was generally greater at temperatures above 0°C than at temperatures below 0°C. Finally, the highest class separability was observed when the temperature was at or above 0°C and there was no snow on the ground or trees.
Research on the ϕ-OTDR fiber sensor sensitive for all of the distance
NASA Astrophysics Data System (ADS)
Kong, Yong; Liu, Yang; Shi, Yi; Ansari, Farhad; Taylor, Todd
2018-01-01
In this paper, a modified construction for the traditional ϕ-OTDR fiber sensor sensitive for all of distance is presented, the related numerical simulation and experiment analysis results show that this construction can reduce the gain imbalance for all of the distance along the fiber caused by the Rayleigh scattering loss of the fiber and the gain imbalance of Raman fiber amplifier in this fiber sensor system. In order to improve further the vibration sensitivity of this system, the possible methods to restrain the influences of modulation instability effect, Stimulated Brillouin effect, reduce the amplified spontaneous emission (ASE) noises of Raman laser (RL) and Erbium3+-doped fiber amplifiers (EDFA), double Rayleigh backscattering noise in this system are discussed, which will offer a great reference value for the science research and engineering application in the field of fiber sensor as we believe.
NASA Astrophysics Data System (ADS)
Razenkov, I. A.
2017-11-01
Micro pulse lasers have allowed solution of some technical problems and design of a specialized aerosol lidar capable of recording backscattering amplification (BSA) in a turbulent atmosphere (2014) by now. The BSA-lidar has two receiving channels, one of which is affected by a turbulence. The measurement result is the ratio of echo signals, i.e., the coefficient of backscattering amplification. The problem of lidar data inversion and retrieval of "optical" turbulence parameters was recently solved by V.V. Vorob'ev theoretically (2016). A lidar experiment was organized for testing the solution, and the asymptotic solution was applied to echo signals, which allowed estimating the daily behavior of the structural characteristics Cn 2 along a horizontal 2-km path. The experiment was accompanied by parallel independent measurements of Cn 2 by an image jitter sensor along the same path. It was shown experimentally that the Vorob'ev solution is applicable to Cn 2 retrieval from BSA-lidar data if β0 2<=3 for β0 2>3, the saturation of the amplification effect and a decrease in the experimental data with respect to calculation results are observed. The coefficient of correlation between the retrieved structural characteristics Cn 2 of the lidar and jitter sensor is 0.8-0.9. The Cn 2 values retrieved from lidar signals turned out to be 20-40% lower than the Cn 2 values of the image jitter sensor.
Roselle is cardioprotective in diet-induced obesity rat model with myocardial infarction.
Si, Lislivia Yiang-Nee; Ali, Siti Aishah Mohd; Latip, Jalifah; Fauzi, Norsyahida Mohd; Budin, Siti Balkis; Zainalabidin, Satirah
2017-12-15
Obesity increase the risks of hypertension and myocardial infarction (MI) mediated by oxidative stress. This study was undertaken to investigate the actions of roselle aqueous extract (R) on cardiotoxicity in obese (OB) rats and thereon OB rats subjected to MI. Male Sprague-Dawley rats were fed with either normal diet or high-fat diet for 8weeks. Firstly, OB rats were divided into (1) OB and (2) OB+R (100mg/kg, p.o, 28days). Then, OB rats were subjected to MI (ISO, 85mg/kg, s.c, 2days) and divided into three groups: (1) OB+MI, (2) OB+MI+R and (3) OB+MI+enalapril for another 4weeks. Roselle ameliorated OB and OB+MI's cardiac systolic dysfunction and reduced cardiac hypertrophy and fibrosis. The increased oxidative markers and decreased antioxidant enzymes in OB and OB+MI groups were all attenuated by roselle. These observations indicate the protective effect of roselle on cardiac dysfunction in OB and OB+MI rats, which suggest its potential to be developed as a nutraceutical product for obese and obese patients with MI in the future. Copyright © 2017 Elsevier Inc. All rights reserved.
Optical backscattering properties of the "clearest" natural waters
NASA Astrophysics Data System (ADS)
Twardowski, M. S.; Claustre, H.; Freeman, S. A.; Stramski, D.; Huot, Y.
2007-11-01
During the BIOSOPE field campaign October-December 2004, measurements of inherent optical properties from the surface to 500 m depth were made with a ship profiler at stations covering over 8000 km through the Southeast Pacific Ocean. Data from a ~3000 km section containing the very clearest waters in the central gyre are reported here. The total volume scattering function at 117°, βt(117°), was measured with a WET Labs ECO-BB3 sensor at 462, 532, and 650 nm with estimated uncertainties of 2×10-5, 5×10-6, and 2×10-6 m-1 sr-1, respectively. These values were approximately 6%, 3%, and 3% of the volume scattering by pure seawater at their respective wavelengths. From a methodological perspective, there were several results: - distributions were resolvable even though some of the values from the central gyre were an order of magnitude lower than the lowest previous measurements in the literature; - Direct in-situ measurements of instrument dark offsets were necessary to accurately resolve backscattering at these low levels; - accurate pure seawater backscattering values are critical in determining particulate backscattering coefficients in the open ocean (not only in these very clear waters); the pure water scattering values determined by Buiteveld et al. (1994) with a [1+0.3S/37] adjustment for salinity based on Morel (1974) appear to be the most accurate estimates, with aggregate accuracies as low as a few percent; and - closure was demonstrated with subsurface reflectance measurements reported by Morel et al. (2007) within instrument precisions, a useful factor in validating the backscattering measurements. This methodology enabled several observations with respect to the hydrography and the use of backscattering as a biogeochemical proxy: -The clearest waters sampled were found at depths between 300 and 350 m, from 23.5° S, 118° W to 26° S, 114° W, where total backscattering at 650 nm was not distinguishable from pure seawater; -Distributions of particulate backscattering bbp across the central gyre exhibited a broad particle peak centered ~100 m; -The particulate backscattering ratio typically ranged between 0.4% and 0.6% at 650 nm through the majority of the central gyre from the surface to ~210 m, indicative of "soft" water-filled particles with low bulk refractive index; and - bbp showed a distinct secondary deeper layer centered ~230 m that was absent in particulate attenuation cp data. The particulate backscattering ratio was significantly higher in this layer than in the rest of the water column, reaching 1.2% in some locations. This high relative backscattering, along with the pigment composition and ecological niche of this layer, appear to be consistent with the coccolithophorid Florisphaera profunda. Moreover, results were consistent with several expectations extrapolated from theory and previous work in oceanic and coastal regions, supporting the conclusion that particulate and total backscattering could be resolved in these extremely clear natural waters.
Optical backscattering properties of the "clearest" natural waters
NASA Astrophysics Data System (ADS)
Twardowski, M. S.; Claustre, H.; Freeman, S. A.; Stramski, D.; Huot, Y.
2007-07-01
During the BIOSOPE field campaign October-December 2004, measurements of inherent optical properties from the surface to 500 m depth were made with a ship profiler at stations covering over ~8000 km through the Southeast Pacific Ocean. Data from a ~3000 km section containing the very clearest waters in the central gyre are reported here. The total volume scattering function at 117°, βt(117°), was measured with a WET Labs ECO-BB3 sensor at 462, 532, and 650 nm with estimated uncertainties of 2×10-5, 5×10-6, and 2×10-6 m-1 sr-1, respectively. These values were approximately 6%, 3%, and 3% of the scattering by pure seawater at their respective wavelengths. From a methodological perspective, there were several results: - bbp distributions were resolvable even though some of the values from the central gyre were an order of magnitude lower than the lowest previous measurements in the literature; - Direct in-situ measurements of instrument dark offsets were necessary to accurately resolve backscattering at these low levels; - accurate pure seawater backscattering values are critical in determining particulate backscattering coefficients in the open ocean (not only in these very clear waters); the pure water scattering values determined by Buiteveld et al. (1994) with a [1 + 0.3S/37] adjustment for salinity based on Morel (1974) appear to be the most accurate estimates, with aggregate accuracies as low as a few percent; and - closure was demonstrated with subsurface reflectance measurements reported by Morel et al. (2007) within instrument precisions, a useful factor in validating the backscattering measurements. This methodology enabled several observations with respect to the hydrography and the use of backscattering as a biogeochemical proxy: - The clearest waters sampled were found at depths between 300 and 350 m, from 23.5° S, 118° W to 26° S, 114° W, where total backscattering at 650 nm was not distinguishable from pure seawater; - Distributions of particulate backscattering bbp across the central gyre exhibited a broad particle peak centered ~100 m; - The particulate backscattering ratio typically ranged between 0.4% and 0.6% through the majority of the central gyre from the surface to ~210 m, indicative of "soft" water-filled particles with low bulk refractive index; and - bbp at 532 and 650 nm showed a distinct secondary deeper layer centered ~230 m that was absent in particulate attenuation cp data. The particulate backscattering ratio was significantly higher in this layer than in the rest of the water column, reaching 1.2% in some locations. This high relative backscattering, along with the pigment composition and ecological niche of this layer, appear to be consistent with the coccolithophorid F. profunda. Moreover, results were consistent with several expectations extrapolated from theory and previous work in oceanic and coastal regions, supporting the conclusion that particulate and total backscattering could be resolved in these extremely clear natural waters.
NASA Astrophysics Data System (ADS)
Scholz, John-Robert; Barruol, Guilhem; Fontaine, Fabrice R.; Sigloch, Karin
2016-04-01
To image the upper mantle structure beneath La Réunion hotspot, a large-scale seismic network has been deployed on land and at sea in the frame of the RHUM-RUM project (Réunion Hotspot and Upper Mantle - Réunions Unterer Mantel). This French-German passive seismic experiment was designed to investigate and image the deep structure beneath La Réunion, from crust to core, to precise the shape and depth origin of a mantle plume, if any, and to precise the horizontal and vertical mantle flow associated to a possible plume upwelling, to its interaction with the overlying plate and with the neighboring Indian ridges. For this purpose, 57 Ocean-Bottom Seismometers (OBS) were installed around La Réunion and along the Central and Southwest Indian ridges. Broad-band instruments were deployed with the French R/V Marion Dufresne in late 2012 (cruise MD192), and recovered 13 months later by the German R/V Meteor (cruise M101). The pool of OBS was complemented by ~60 terrestrial stations, installed on different islands in the western Indian Ocean, such as La Réunion, Madagascar, Mauritius, Seychelles, Mayotte and the Îles Éparses in the Mozambique channel. The OBS installation is a free-fall down to the seafloor, where they landed in an unknown orientation. Since seismologic investigations of crustal and upper mantle structure (e.g., receiver functions) and azimuthal anisotropy (e.g., SKS-splitting and Rayleigh waves) rely on the knowledge of the correct OBS orientation with respect to the geographic reference frame, it is of importance to determine the orientations of the OBS while recording on the seafloor. In an isotropic, horizontally homogeneous and non-dipping layered globe, the misorientation of each station refers to the offset between theoretical and recorded back-azimuth angle of a passive seismic event. Using large earthquakes (MW > 5.0), it is possible to establish multiple successful measurements per station and thus to determine with good confidence the sensor orientations. In this study, we analyzed particle motions of P-waves (P-pol) and of Rayleigh waves (R-pol) to quantify the orientation of each of the 57 OBS. We performed 213 polarization measurements based on 35 earthquakes for P-pol, and 381 polarization measurements based on 48 earthquakes for R-pol. This allowed us to successfully determine the North/South orientations for 40 out of 57 OBS: 13 stations were devoid of usable data and 4 stations were too noisy. From twice the standard deviation (95% confidence interval), we estimate the errors between 1° and 20°.
NASA Astrophysics Data System (ADS)
Jamlos, Mohd Aminudin; Ismail, Abdul Hafiizh; Jamlos, Mohd Faizal; Narbudowicz, Adam
2017-01-01
Hybrid graphene-copper ultra-wideband array sensor applied to microwave imaging technique is successfully used in detecting and visualizing tumor inside human brain. The sensor made of graphene coated film for the patch while copper for both the transmission line and parasitic element. The hybrid sensor performance is better than fully copper sensor. Hybrid sensor recorded wider bandwidth of 2.0-10.1 GHz compared with fully copper sensor operated from 2.5 to 10.1 GHz. Higher gain of 3.8-8.5 dB is presented by hybrid sensor, while fully copper sensor stated lower gain ranging from 2.6 to 6.7 dB. Both sensors recorded excellent total efficiency averaged at 97 and 94%, respectively. The sensor used for both transmits equivalent signal and receives backscattering signal from stratified human head model in detecting tumor. Difference in the data of the scattering parameters recorded from the head model with presence and absence of tumor is used as the main data to be further processed in confocal microwave imaging algorithm in generating image. MATLAB software is utilized to analyze S-parameter signals obtained from measurement. Tumor presence is indicated by lower S-parameter values compared to higher values recorded by tumor absence.
Peng, Wen Yu; Goldenstein, Christopher S; Mitchell Spearrin, R; Jeffries, Jay B; Hanson, Ronald K
2016-11-20
The development and demonstration of a four-color single-ended mid-infrared tunable laser-absorption sensor for simultaneous measurements of H2O, CO2, CO, and temperature in combustion flows is described. This sensor operates by transmitting laser light through a single optical port and measuring the backscattered radiation from within the combustion device. Scanned-wavelength-modulation spectroscopy with second-harmonic detection and first-harmonic normalization (scanned-WMS-2f/1f) was used to account for variable signal collection and nonabsorption losses in the harsh environment. Two tunable diode lasers operating near 2551 and 2482 nm were utilized to measure H2O concentration and temperature, while an interband cascade laser near 4176 nm and a quantum cascade laser near 4865 nm were used for measuring CO2 and CO, respectively. The lasers were modulated at either 90 or 112 kHz and scanned across the peaks of their respective absorption features at 1 kHz, leading to a measurement rate of 2 kHz. A hybrid demultiplexing strategy involving both spectral filtering and frequency-domain demodulation was used to decouple the backscattered radiation into its constituent signals. Demonstration measurements were made in the exhaust of a laboratory-scale laminar methane-air flat-flame burner at atmospheric pressure and equivalence ratios ranging from 0.7 to 1.2. A stainless steel reflective plate was placed 0.78 cm away from the sensor head within the combustion exhaust, leading to a total absorption path length of 1.56 cm. Detection limits of 1.4% H2O, 0.6% CO2, and 0.4% CO by mole were reported. To the best of the authors' knowledge, this work represents the first demonstration of a mid-infrared laser-absorption sensor using a single-ended architecture in combustion flows.
Simulation Studies of Forest Structure using 3D Lidar and Radar Models
NASA Technical Reports Server (NTRS)
Sun, Guoqing; Ranson, K. Jon; Koetz, Benjamin; Liu, Dawei
2007-01-01
The use of lidars and radars to measure forest structure attributes such as height and biomass are being considered for future Earth Observation missions. Large footprint lidar makes a direct measurement of the heights of scatterers in the illuminated footprint and can yield information about the vertical profile of the canopy. Synthetic Aperture Radar (SAR) is known to sense the canopy volume, especially at longer wavelengths and is useful for estimating biomass. Interferometric SAR (InSAR) has been shown to yield forest canopy height information. For example, the height of scattering phase retrieved from InSAR data is considered to be correlated with the three height and the spatial structure of the forest stand. There is much interest in exploiting these technologies separately and together to get important information for carbon cycle and ecosystem science. More detailed information of the electromagnetic radiation interactions within forest canopies is needed. And backscattering models can be of much utility here. As part of a NASA funded project to explore data fusion, a three-dimensional (3D) coherent radar backscattering model and a 3D lidar backscatter models were used to investigate the use of large footprint lidar, SAR and InSAR for characterizing realistic forest scenes. For this paper, we use stem maps and other forest measurements to develop a realistic spatial structure of a spruce-hemlock forest canopy found in Maine, USA. The radar and lidar models used measurements of the 3D forest scene as input and simulated the coherent radar backscattering signature and 1064nm energy backscatter, respectively. The relationships of backscatter derived forest structure were compared with field measurements. In addition, we also had detailed airborne lidar (Laser Imaging Vegetation Sensor, LVIS) data available over the stem map sites that was used to study the accuracies of tree height derived from modeled SAR backscatter and the scattering phase center retrieved from the simulated InSAR data will be compared with the height indices, or other structure parameters derived from the lidar data. These results will address the possible synergies between lidar and radar in data in terms of forest structural information.
2010-09-29
1 1 1 2 2 2ˆ ˆ ˆ ˆ ˆ ˆˆ ˆ ˆ ˆ0 0 0 0 0 0 0 0 0 0 T r ob ob ob ob ob ob tr tr trX r r rθ φ θ φ θ φ= (3.28) 29...ˆ ˆ ˆ ˆˆ ˆ ˆ0 0 0 0 0 0 0 T r ob ob ob tr tr trX r rθ φ θ φ⎡ ⎤= ⎣ ⎦ (3.29) Since obstacles are assumed to be point obstacles, it
Shallow water acoustic backscatter and reverberation measurements using a 68-kHz cylindrical array
NASA Astrophysics Data System (ADS)
Gallaudet, Timothy Cole
2001-10-01
The characterization of high frequency, shallow water acoustic backscatter and reverberation is important because acoustic systems are used in many scientific, commercial, and military applications. The approach taken is to use data collected by the Toroidal Volume Search Sonar (TVSS), a 68 kHz multibeam sonar capable of 360° imaging in a vertical plane perpendicular to its direction of travel. With this unique capability, acoustic backscatter imagery of the seafloor, sea surface, and horizontal and vertical planes in the volume are constructed from data obtained in 200m deep waters in the Northeastern Gulf of Mexico when the TVSS was towed 78m below the surface, 735m astern of a towship. The processed imagery provide a quasi-synoptic characterization of the spatial and temporal structure of boundary and volume acoustic backscatter and reverberation. Diffraction, element patterns, and high sidelobe levels are shown to be the most serious problems affecting cylindrical arrays such as the TVSS, and an amplitude shading method is presented for reducing the peak sidelobe levels of irregular-line and non-coplanar arrays. Errors in the towfish's attitude and motion sensor, and irregularities in the TVSS's transmitted beampattern produce artifacts in the TVSS-derived bathymetry and seafloor acoustic backscatter imagery. Correction strategies for these problems are described, which are unique in that they use environmental information extracted from both ocean boundaries. Sea surface and volume acoustic backscatter imagery are used to explore and characterize the structure of near-surface bubble clouds, schooling fish, and zooplankton. The simultaneous horizontal and vertical coverage provided by the TVSS is shown to be a primary advantage, motivating further use of multibeam sonars in these applications. Whereas boundary backscatter fluctuations are well described by Weibull, K, and Rayleigh mixture probability distributions, those corresponding to volume backscatter are multi-modal, with the log-normal distribution providing the best fits to the centers of the distributions, and the Rayleigh mixture models providing the best fits to the tails of the distributions. The largest distribution tails result from resonant microbubbles and patchy aggregations of zooplankton. The Office of Naval Research funded this work under ONR-NRL Contract No. N00014-96-1-G9I3.
A finger-free wrist-worn pulse oximeter for the monitoring of chronic obstructive pulmonary disease
NASA Astrophysics Data System (ADS)
Chu, Chang-Sheng; Chuang, Shuang-Chao; Lee, Yeh Wen; Fan, Chih-Hsun; Chung, Lung Pin; Li, Yu-Tang; Chen, Jyh-Chern
2016-03-01
Herein, a finger-free wrist-worn pulse oximeter is presented. This device allows patients to measure blood oxygen level and pulse rate without hindering their normal finger movement. This wrist-worn pulse oximeter is built with a reflectance oximetry sensor, which consists of light emitting diodes and photodiode light detectors located side by side. This reflectance oximetry sensor is covered with an optical element with micro structured surface. This micro structured optical element is designed to modulate photon propagation beneath the skin tissue so that the photoplethysmogram signals of reflected lights or backscattered lights detected by the photodetector are therefore enhanced.
NASA Technical Reports Server (NTRS)
Schmullius, C.; Nithack, J.
1992-01-01
On July 12, the MAC Europe '91 (Multi-Sensor Airborne Campaign) took place over test site Oberpfaffenhofen. The DLR Institute of Radio-Frequency Technology participated with its C-VV, X-VV, and X-HH Experimental Synthetic Aperture Radar (E-SAR). The high resolution E-SAR images with a pixel size between 1 and 2 m and the polarimetric AIRSAR images were analyzed. Using both sensors in combination is a unique opportunity to evaluate SAR images in a frequency range from P- to X-band and to investigate polarimetric information.
Högberg, Helena; Engblom, Lars; Ekdahl, Asa; Lidell, Veronica; Walum, Erik; Alberts, Peteris
2006-04-01
The aims were to compare the temperature dependence of the metabolic rate in young ob/ob mice with that in mature ob/ob and db/db mice and to examine the effect on the metabolic substrate preference of leptin and etomoxir in ob/ob, C57BL/6J (wild-type), and db/db mice. In vivo oxygen consumption and carbon dioxide production were continuously measured by indirect calorimetry, and body temperature and total locomotor activity were measured by an implanted transponder. Leptin, etomoxir, or vehicle was administered intraperitoneally. The temperature dependence of the metabolic rate of mature ob/ob and db/db mice were similar to that in wild-type mice. In young 6-week-old ob/ob mice, the metabolic rate was almost doubled at 15 degrees C. Leptin (2 x 3 mg/kg) decreased the respiratory quotient (RQ) and carbon dioxide production but did not alter oxygen consumption, body temperature, or locomotor activity in ob/ob and C57BL/6J mice and had no effect in the db/db mice. Etomoxir (2 x 30 mg/kg) enhanced RQ and decreased oxygen consumption, carbon dioxide production, and body temperature in ob/ob, C57BL/6J, and db/db mice. Total locomotor activity was reduced in ob/ob and C57BL/6J mice. In young ob/ob mice, the temperature sensitivity was enhanced compared with mature mice. Leptin and etomoxir had opposite effects on metabolic substrate preference. Leptin and lowered environmental temperature increased the relative fat oxidation as indicated by decreased RQ, possibly through activation of the sympathetic nervous system.
Martins, Fabiane Ferreira; Bargut, Thereza Cristina Lonzetti; Aguila, Marcia Barbosa; Mandarim-de-Lacerda, Carlos Alberto
2017-03-01
Brown adipose tissue (BAT) is specialized in heat production, but its metabolism in ob/ob mice is still a matter of debate. We aimed to verify ob/ob mice BAT using C57Bl/6 male mice (as the wild-type, WT) and leptin-deficient ob/ob mice (on the C57Bl/6 background strain), at three months of age (n=10/group). At euthanasia, animals had their interscapular BAT weighed, and prepared for analysis (Western blot, and RT-qPCR). In comparison with the WT group, the ob/ob group showed reduced thermogenic signaling markers (gene expression of beta 3-adrenergic receptor, beta3-AR; PPARgamma coactivator 1 alpha, PGC1alpha, and uncoupling protein 1, UCP1). The ob/ob group also showed impaired gene expression for lipid utilization (perilipin was increased, while other markers were diminished: carnitine palmitoyltransferase-1b, CPT-1b; cluster of differentiation 36, CD36; fatty acid binding protein 4, FABP4; fatty acid synthase, FAS, and sterol regulatory element-binding protein 1c, SREBP1c), and altered protein expression of insulin signaling (diminished pAKT, TC10, and GLUT-4). Lastly, the ob/ob group showed increased gene expression of markers of inflammation (interleukin 1 beta, IL-1beta; IL-6, tumor necrosis factor alpha, TNFalpha; and monocyte chemotactic protein-1, MCP-1). In conclusion, the ob/ob mice have decreased thermogenic markers associated with reduced gene expression related to fatty acid synthesis, mobilization, and oxidation. There were also alterations in insulin signaling and protein and gene expressions of inflammation. The findings suggest that the lack of substrate for thermogenesis and the local inflammation negatively regulated thermogenic signaling in the ob/ob mice. Copyright © 2016 Elsevier GmbH. All rights reserved.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Sekiya, Motohiro; Yahagi, Naoya, E-mail: nyahagi-tky@umin.ac.jp; Laboratory of Molecular Physiology on Energy Metabolism, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655
2009-09-25
It has long been a matter of debate whether the hormone-sensitive lipase (HSL)-mediated lipolysis in pancreatic {beta}-cells can affect insulin secretion through the alteration of lipotoxicity. We generated mice lacking both leptin and HSL (Lep{sup ob/ob}/HSL{sup -/-}) and explored the role of HSL in pancreatic {beta}-cells in the setting of obesity. Lep{sup ob/ob}/HSL{sup -/-} developed elevated blood glucose levels and reduced plasma insulin levels compared with Lep{sup ob/ob}/HSL{sup +/+} in a fed state, while the deficiency of HSL did not affect glucose homeostasis in Lep{sup +/+} background. The deficiency of HSL exacerbated the accumulation of triglycerides in Lep{sup ob/ob} islets,more » leading to reduced glucose-stimulated insulin secretion. The deficiency of HSL also diminished the islet mass in Lep{sup ob/ob} mice due to decreased cell proliferation. In conclusion, HSL affects insulin secretary capacity especially in the setting of obesity.« less
Energy balance in adrenalectomized ob/ob mice: effects of dietary starch and glucose.
Warwick, B P; Romsos, D R
1988-07-01
Effects of different carbohydrate types on energy balance, fatty acid synthesis, and plasma insulin concentrations in adrenalectomized ob/ob mice were investigated. Obese (ob/ob) and lean mice adrenalectomized at 4 wk of age received one of four high-carbohydrate powdered diets for 3 wk: stock, glucose, starch, or starch plus wheat bran. Adrenalectomy reduced energy intake of ob/ob mice equally independent of diet type, whereas energetic efficiency, in vivo rates of fatty acid synthesis in liver and white adipose tissue, and plasma insulin concentrations were substantially reduced to approach values in lean mice in all adrenalectomized ob/ob mice except those fed glucose. The ability of adrenalectomy to normalize energy balance in ob/ob mice depends on factors other than the reduced circulating concentration of glucocorticoids alone. Diet composition is a crucial factor, and striking differences exist between semipurified diets containing a simple sugar (glucose) and those containing a complex carbohydrate (starch), with no additional effect of dietary fiber (wheat bran).
Dielectrically-Loaded Cylindrical Resonator-Based Wireless Passive High-Temperature Sensor
Xiong, Jijun; Wu, Guozhu; Tan, Qiulin; Wei, Tanyong; Wu, Dezhi; Shen, Sanmin; Dong, Helei; Zhang, Wendong
2016-01-01
The temperature sensor presented in this paper is based on a microwave dielectric resonator, which uses alumina ceramic as a substrate to survive in harsh environments. The resonant frequency of the resonator is determined by the relative permittivity of the alumina ceramic, which monotonically changes with temperature. A rectangular aperture etched on the surface of the resonator works as both an incentive and a coupling device. A broadband slot antenna fed by a coplanar waveguide is utilized as an interrogation antenna to wirelessly detect the sensor signal using a radio-frequency backscattering technique. Theoretical analysis, software simulation, and experiments verified the feasibility of this temperature-sensing system. The sensor was tested in a metal-enclosed environment, which severely interferes with the extraction of the sensor signal. Therefore, frequency-domain compensation was introduced to filter the background noise and improve the signal-to-noise ratio of the sensor signal. The extracted peak frequency was found to monotonically shift from 2.441 to 2.291 GHz when the temperature was varied from 27 to 800 °C, leading to an average absolute sensitivity of 0.19 MHz/°C. PMID:27916920
Towards Enhanced Underwater Lidar Detection via Source Separation
NASA Astrophysics Data System (ADS)
Illig, David W.
Interest in underwater optical sensors has grown as technologies enabling autonomous underwater vehicles have been developed. Propagation of light through water is complicated by the dual challenges of absorption and scattering. While absorption can be reduced by operating in the blue-green region of the visible spectrum, reducing scattering is a more significant challenge. Collection of scattered light negatively impacts underwater optical ranging, imaging, and communications applications. This thesis concentrates on the ranging application, where scattering reduces operating range as well as range accuracy. The focus of this thesis is on the problem of backscatter, which can create a "clutter" return that may obscure submerged target(s) of interest. The main contributions of this thesis are explorations of signal processing approaches to increase the separation between the target and backscatter returns. Increasing this separation allows detection of weak targets in the presence of strong scatter, increasing both operating range and range accuracy. Simulation and experimental results will be presented for a variety of approaches as functions of water clarity and target position. This work provides several novel contributions to the underwater lidar field: 1. Quantification of temporal separation approaches: While temporal separation has been studied extensively, this work provides a quantitative assessment of the extent to which both high frequency modulation and spatial filter approaches improve the separation between target and backscatter. 2. Development and assessment of frequency separation: This work includes the first frequency-based separation approach for underwater lidar, in which the channel frequency response is measured with a wideband waveform. Transforming to the time-domain gives a channel impulse response, in which target and backscatter returns may appear in unique range bins and thus be separated. 3. Development and assessment of statistical separation: The first investigations of statistical separation approaches for underwater lidar are presented. By demonstrating that target and backscatter returns have different statistical properties, a new separation axis is opened. This work investigates and quantifies performance of three statistical separation approaches. 4. Application of detection theory to underwater lidar: While many similar applications use detection theory to assess performance, less development has occurred in the underwater lidar field. This work applies these concepts to statistical separation approaches, providing another perspective in which to assess performance. In addition, by using detection theory approaches, statistical metrics can be used to associate a level of confidence in each ranging measurement. 5. Preliminary investigation of forward scatter suppression: If backscatter is sufficiently suppressed, forward scattering becomes a performance-limiting factor. This work presents a proof-of-concept demonstration of the potential for statistical separation approaches to suppress both forward and backward scatter. These results provide a demonstration of the capability that signal processing has to improve separation between target and backscatter. Separation capability improves in the transition from temporal to frequency to statistical separation approaches, with the statistical separation approaches improving target detection sensitivity by as much as 30 dB. Ranging and detection results demonstrate the enhanced performance this would allow in ranging applications. This increased performance is an important step in moving underwater lidar capability towards the requirements of the next generation of sensors.
Loss of stearoyl-CoA desaturase 1 rescues cardiac function in obese leptin-deficient mice.
Dobrzyn, Pawel; Dobrzyn, Agnieszka; Miyazaki, Makoto; Ntambi, James M
2010-08-01
The heart of leptin-deficient ob/ob mice is characterized by pathologic left ventricular hypertrophy along with elevated triglyceride (TG) content, increased stearoyl-CoA desaturase (SCD) activity, and increased myocyte apoptosis. In the present study, using an ob/ob;SCD1(-/-) mouse model, we tested the hypothesis that lack of SCD1 could improve steatosis and left ventricle (LV) function in leptin deficiency. We show that disruption of the SCD1 gene improves cardiac function in ob/ob mice by correcting systolic and diastolic dysfunction without affecting levels of plasma TG and FFA. The improvement is associated with reduced expression of genes involved in FA transport and lipid synthesis in the heart, as well as reduction in cardiac FFA, diacylglycerol, TG, and ceramide levels. The rate of FA beta-oxidation is also significantly lower in the heart of ob/ob;SCD1(-/-) mice compared with ob/ob controls. Moreover, SCD1 deficiency reduces cardiac apoptosis in ob/ob mice due to increased expression of antiapoptotic factor Bcl-2 and inhibition of inducible nitric oxide synthase and caspase-3 activities. Reduction in myocardial lipid accumulation and inhibition of apoptosis appear to be one of the main mechanisms responsible for improved LV function in ob/ob mice caused by SCD1 deficiency.
Schaab, Michael; Kausch, Henriette; Klammt, Juergen; Nowicki, Marcin; Anderegg, Ulf; Gebhardt, Rolf; Rose-John, Stefan; Scheller, Juergen; Thiery, Joachim; Kratzsch, Juergen
2012-01-01
The adipokine leptin realizes signal transduction via four different membrane-anchored leptin receptor (Ob-R) isoforms in humans. However, the amount of functionally active Ob-R is affected by constitutive shedding of the extracellular domain via a so far unknown mechanism. The product of the cleavage process the so-called soluble leptin receptor (sOb-R) is the main binding protein for leptin in human blood and modulates its bioavailability. sOb-R levels are differentially regulated in metabolic disorders like type 1 diabetes mellitus or obesity and can, therefore, enhance or reduce leptin sensitivity. To describe mechanisms of Ob-R cleavage and to investigate the functional significance of differential sOb-R levels we established a model of HEK293 cells transiently transfected with different human Ob-R isoforms. Using siRNA knockdown experiments we identified ADAM10 (A Disintegrin And Metalloproteinase 10) as a major protease for constitutive and activated Ob-R cleavage. Additionally, the induction of lipotoxicity and apoptosis led to enhanced shedding shown by increased levels of the soluble leptin receptor (sOb-R) in cell supernatants. Conversely, high leptin concentrations and ER stress reduced sOb-R levels. Decreased amounts of sOb-R due to ER stress were accompanied by impaired leptin signaling and reduced leptin binding. Lipotoxicity and apoptosis increased Ob-R cleavage via ADAM10-dependent mechanisms. In contrast high leptin levels and ER stress led to reduced sOb-R levels. While increased sOb-R concentrations seem to directly block leptin action, reduced amounts of sOb-R may reflect decreased membrane expression of Ob-R. These findings could explain changes of leptin sensitivity which are associated with variations of serum sOb-R levels in metabolic diseases.
Schaab, Michael; Kausch, Henriette; Klammt, Juergen; Nowicki, Marcin; Anderegg, Ulf; Gebhardt, Rolf; Rose-John, Stefan; Scheller, Juergen; Thiery, Joachim; Kratzsch, Juergen
2012-01-01
Background The adipokine leptin realizes signal transduction via four different membrane-anchored leptin receptor (Ob-R) isoforms in humans. However, the amount of functionally active Ob-R is affected by constitutive shedding of the extracellular domain via a so far unknown mechanism. The product of the cleavage process the so-called soluble leptin receptor (sOb-R) is the main binding protein for leptin in human blood and modulates its bioavailability. sOb-R levels are differentially regulated in metabolic disorders like type 1 diabetes mellitus or obesity and can, therefore, enhance or reduce leptin sensitivity. Methodology/Principal Findings To describe mechanisms of Ob-R cleavage and to investigate the functional significance of differential sOb-R levels we established a model of HEK293 cells transiently transfected with different human Ob-R isoforms. Using siRNA knockdown experiments we identified ADAM10 (A Disintegrin And Metalloproteinase 10) as a major protease for constitutive and activated Ob-R cleavage. Additionally, the induction of lipotoxicity and apoptosis led to enhanced shedding shown by increased levels of the soluble leptin receptor (sOb-R) in cell supernatants. Conversely, high leptin concentrations and ER stress reduced sOb-R levels. Decreased amounts of sOb-R due to ER stress were accompanied by impaired leptin signaling and reduced leptin binding. Conclusions Lipotoxicity and apoptosis increased Ob-R cleavage via ADAM10-dependent mechanisms. In contrast high leptin levels and ER stress led to reduced sOb-R levels. While increased sOb-R concentrations seem to directly block leptin action, reduced amounts of sOb-R may reflect decreased membrane expression of Ob-R. These findings could explain changes of leptin sensitivity which are associated with variations of serum sOb-R levels in metabolic diseases. PMID:22545089
Characterization of Forest Opacity Using Multi-Angular Emission and Backscatter Data
NASA Technical Reports Server (NTRS)
Kurum, Mehmet; O'Neill, Peggy; Lang, Roger H.; Joseph, Alicia T.; Cosh, Michael H.; Jackson, Thomas J.
2010-01-01
This paper discusses the results from a series of field experiments using ground-based L-band microwave active/passive sensors. Three independent approaches are employed to the microwave data to determine vegetation opacity of coniferous trees. First, a zero-order radiative transfer model is fitted to multi-angular microwave emissivity data in a least-square sense to provide "effective" vegetation optical depth. Second, a ratio between radar backscatter measurements with the corner reflector under trees and in an open area is calculated to obtain "measured" tree propagation characteristics. Finally, the "theoretical" propagation constant is determined by forward scattering theorem using detailed measurements of size/angle distributions and dielectric constants of the tree constituents (trunk, branches, and needles). The results indicate that "effective" values underestimate attenuation values compared to both "theoretical" and "measured" values.
NASA Astrophysics Data System (ADS)
Burnett, T. L.; Weaver, P. M.; Blackburn, J. F.; Stewart, M.; Cain, M. G.
2010-08-01
The functional properties of ferroelectric ceramic bulk or thin film materials are strongly influenced by their nanostructure, crystallographic orientation, and structural geometry. In this paper, we show how, by combining textural analysis, through electron backscattered diffraction, with piezoresponse force microscopy, quantitative measurements of the piezoelectric properties can be made at a scale of 25 nm, smaller than the domain size. The combined technique is used to obtain data on the domain-resolved effective single crystal piezoelectric response of individual crystallites in Pb(Zr0.4Ti0.6)O3 ceramics. The results offer insight into the science of domain engineering and provide a tool for the future development of new nanostructured ferroelectric materials for memory, nanoactuators, and sensors based on magnetoelectric multiferroics.
LISA pathfinder optical interferometry
NASA Astrophysics Data System (ADS)
Braxmaier, Claus; Heinzel, Gerhard; Middleton, Kevin F.; Caldwell, Martin E.; Konrad, W.; Stockburger, H.; Lucarelli, S.; te Plate, Maurice B.; Wand, V.; Garcia, A. C.; Draaisma, F.; Pijnenburg, J.; Robertson, D. I.; Killow, Christian; Ward, Harry; Danzmann, Karsten; Johann, Ulrich A.
2004-09-01
The LISA Technology Package (LTP) aboard of LISA pathfinder mission is dedicated to demonstrate and verify key technologies for LISA, in particular drag free control, ultra-precise laser interferometry and gravitational sensor. Two inertial sensor, the optical interferometry in between combined with the dimensional stable Glass ceramic Zerodur structure are setting up the LTP. The validation of drag free operation of the spacecraft is planned by measuring laser interferometrically the relative displacement and tilt between two test masses (and the optical bench) with a noise levels of 10pm/√Hz and 10 nrad/√Hz between 3mHz and 30mHz. This performance and additionally overall environmental tests was currently verified on EM level. The OB structure is able to support two inertial sensors (≍17kg each) and to withstand 25 g design loads as well as 0...40°C temperature range. Optical functionality was verified successfully after environmental tests. The engineering model development and manufacturing of the optical bench and interferometry hardware and their verification tests will be presented.
Lange-Consiglio, Anna; Corradetti, Bruna; Perrini, Claudia; Bizzaro, Davide; Cremonesi, Fausto
2016-04-01
In human and swine, leptin (OB) has been identified in seminal plasma and leptin receptors (OB-R) on the cell surface of spermatozoa, indicating that spermatozoa are a target for OB. This hormone has also been detected in follicular fluid (FF) in women and mares, although its role requires further study. The aims of this study were to investigate the immunolocalisation and the expression of OB and OB-R in equine spermatozoa and to evaluate the involvement of OB in equine in vitro fertilisation (IVF). Since progesterone (P) and OB are both found in FF, the individual and combined effects of these two hormones were studied in equine IVF and compared with the results obtained from the use of FF for in vitro sperm preparation. For the first time, we were able to identify OB and OB-R mRNA and their corresponding proteins in equine spermatozoa. When spermatozoa were treated with OB, there was a decrease in the three motility parameters VSL, STR and LIN, commonly associated with hyperactivation, whilst the acrosome reaction rate increased (P<0.05). The fertilisation rate was 51% with FF, 46.15% with P, 43.64% with P+OB and 0% with OB alone. The percentage of eight-cell stage embryos was 18.7% with FF, 17.1% with P and 16.7% with OB+P. OB alone did not permit oocyte fertilisation, indicating that, in the horse, OB is involved in capacitation and hyperactivation but not in sperm penetration.
Rüter, Jens; Hoffmann, Torsten; Demuth, Hans-Ulrich; Moschansky, Petra; Klapp, Burghard F; Hildebrandt, Martin
2004-06-01
We assessed changes of the enzyme dipeptidyl peptidase IV (DPP IV, CD26) in the context of leptin or leptin receptor deficiency. C57BL/6 mice, Leptin-deficient mice (ob/ob mice, B6.V-Lep
Hutchison, R E
1975-10-01
As intact, breeding female budgerigars approach egg-laying, they spend an increasing amount of time in the nestbox and nest hollow. The brood patch area begins to defeather and becomes vascular and the oviduct increases in weight. Precursory albumen forms in the tubular glands of the oviduct. Oestradiol (OB) treatment in combination with prolactin (OB+PL) induced ovariectomized budgerigars to display nesting behaviour which did not differ from that shown by intact females in the 3 days immediately preceding egg-laying. In contrast, OB induced only the initial phase of the nesting sequence and the effects of OB in combination with progesterone (OB+PR) were intermediate between treatments with OB alone and OB+PL. Incubation of artificial eggs occurred only in the OB+PL group and the latency to display of the incubation posture was shorter in the OB+PL group than in the OB+PR group. No incubation posture was displayed by the OB-treated group. Oviduct development was not influenced by prolactin, but progesterone induced precocious development of tubular glands in the magnum region of the oviduct. Treatment with OB+PR induced uniform development of precursor albumen in the tubular glands. Development of the brood patch occurred with both OB+PL and OB+PR treatment. However, OB+PR resulted in defeathering which was advanced in relation to vascularity when compared with intact breeding females, whereas defeathering and vascularity of the OB+PL group did not differ from that of intact females at egg-laying. These results indicate that prolactin in combination with oestradiol was more effective than progesterone not only in inducing the later phases of nesting behaviour but also in initiating incubation behaviour and defeathering of the brood patch area.
Sennello, Joseph A.; Fayad, Raja; Pini, Maria; Gove, Melissa E.; Ponemone, Venkatesh; Cabay, Robert J.; Siegmund, Britta; Dinarello, Charles A.; Fantuzzi, Giamila
2008-01-01
Obesity is associated with increased severity of acute pancreatitis (AP). The cytokines IL-18 and IL-12 are elevated in patients with AP, and IL-18 levels are high in obesity. We aimed to develop a pathologically relevant model to study obesity-associated severe AP. Lean WT and obese leptin-deficient ob/ob mice received two injections of IL-12 plus IL-18. Survival, pancreatic inflammation, and biochemical markers of AP were measured. Dosing with IL-12 plus IL-18 induced 100% lethality in ob/ob mice; no lethality was observed in WT mice. Disruption of pancreatic exocrine tissue and acinar cell death as well as serum amylase and lipase levels were significantly higher in ob/ob than in WT mice. Edematous AP developed in WT mice, whereas obese ob/ob mice developed necrotizing AP. Adipose tissue necrosis and saponification were present in cytokine-injected ob/ob but not in WT mice. Severe hypocalcemia and elevated acute-phase response developed in ob/ob mice. The cytokine combination induced high levels of regenerating protein 1 and pancreatitis-associated protein expression in the pancreas of WT but not of ob/ob mice. To differentiate the contribution of obesity to that of leptin deficiency, mice received short- and long-term leptin replacement therapy. Short-term leptin reconstitution in the absence of major weight loss did not protect ob/ob mice, whereas leptin deficiency in the absence of obesity resulted in a significant reduction in the severity of the pancreatitis. In conclusion, we developed a pathologically relevant model of AP in which obesity per se is associated with increased severity. PMID:18515422
Accurate estimation of sigma(exp 0) using AIRSAR data
NASA Technical Reports Server (NTRS)
Holecz, Francesco; Rignot, Eric
1995-01-01
During recent years signature analysis, classification, and modeling of Synthetic Aperture Radar (SAR) data as well as estimation of geophysical parameters from SAR data have received a great deal of interest. An important requirement for the quantitative use of SAR data is the accurate estimation of the backscattering coefficient sigma(exp 0). In terrain with relief variations radar signals are distorted due to the projection of the scene topography into the slant range-Doppler plane. The effect of these variations is to change the physical size of the scattering area, leading to errors in the radar backscatter values and incidence angle. For this reason the local incidence angle, derived from sensor position and Digital Elevation Model (DEM) data must always be considered. Especially in the airborne case, the antenna gain pattern can be an additional source of radiometric error, because the radar look angle is not known precisely as a result of the the aircraft motions and the local surface topography. Consequently, radiometric distortions due to the antenna gain pattern must also be corrected for each resolution cell, by taking into account aircraft displacements (position and attitude) and position of the backscatter element, defined by the DEM data. In this paper, a method to derive an accurate estimation of the backscattering coefficient using NASA/JPL AIRSAR data is presented. The results are evaluated in terms of geometric accuracy, radiometric variations of sigma(exp 0), and precision of the estimated forest biomass.
Using SAR satellite data time series for regional glacier mapping
NASA Astrophysics Data System (ADS)
Winsvold, Solveig H.; Kääb, Andreas; Nuth, Christopher; Andreassen, Liss M.; van Pelt, Ward J. J.; Schellenberger, Thomas
2018-03-01
With dense SAR satellite data time series it is possible to map surface and subsurface glacier properties that vary in time. On Sentinel-1A and RADARSAT-2 backscatter time series images over mainland Norway and Svalbard, we outline how to map glaciers using descriptive methods. We present five application scenarios. The first shows potential for tracking transient snow lines with SAR backscatter time series and correlates with both optical satellite images (Sentinel-2A and Landsat 8) and equilibrium line altitudes derived from in situ surface mass balance data. In the second application scenario, time series representation of glacier facies corresponding to SAR glacier zones shows potential for a more accurate delineation of the zones and how they change in time. The third application scenario investigates the firn evolution using dense SAR backscatter time series together with a coupled energy balance and multilayer firn model. We find strong correlation between backscatter signals with both the modeled firn air content and modeled wetness in the firn. In the fourth application scenario, we highlight how winter rain events can be detected in SAR time series, revealing important information about the area extent of internal accumulation. In the last application scenario, averaged summer SAR images were found to have potential in assisting the process of mapping glaciers outlines, especially in the presence of seasonal snow. Altogether we present examples of how to map glaciers and to further understand glaciological processes using the existing and future massive amount of multi-sensor time series data.
Object-based connectedness facilitates matching.
Koning, Arno; van Lier, Rob
2003-10-01
In two matching tasks, participants had to match two images of object pairs. Image-based (IB) connectedness refers to connectedness between the objects in an image. Object-based (OB) connectedness refers to connectedness between the interpreted objects. In Experiment 1, a monocular depth cue (shadow) was used to distinguish different relation types between object pairs. Three relation types were created: IB/OB-connected objects, IB/OB-disconnected objects, and IB-connected/OB-disconnected objects. It was found that IB/OB-connected objects were matched faster than IB/OB-disconnected objects. Objects that were IB-connected/OB-disconnected were matched equally to IB/OB-disconnected objects. In Experiment 2, stereoscopic presentation was used. With relation types comparable to those in Experiment 1, it was again found that OB connectedness determined speed of matching, rather than IB connectedness. We conclude that matching of projections of three-dimensional objects depends more on OB connectedness than on IB connectedness.
Quantitative use of multiincidence-angle SAR for geologic mapping
NASA Technical Reports Server (NTRS)
Farr, T. G.; Albee, A. L.; Evans, D. L.; Solomon, J. E.; Daily, M. I.; Labotka, T. C.; Smith, M. O.
1984-01-01
It is proposed that techniques be developed and used for quantitative interpretation of shuttle imaging radar-B (SIR-B) data for lithologic identification and mapping. The use of backscatter versus incidence angle signatures derived from SIR-B images is to be investigated. The use of SIR-B with other sensors for geologic mapping is also to be considered. Anticipated results are discussed in terms of geologic mapping.
STS-34: Mission Overview Briefing
NASA Technical Reports Server (NTRS)
1989-01-01
Live footage shows Milt Heflin, the Lead Flight Director participating in the STS-34 Mission Briefing. He addresses the primary objective, and answered questions from the audience and other NASA Centers. Heflin also mentions the Shuttle Solar Backscatter Ultraviolet secondary payload, and several experiments. These experiments include Growth Hormone Crystal Distribution (Plants), Polymer Morphology, Sensor Technology Experiment, Mesoscale Lightning Experiment, Shuttle Student Involvement Program "Ice Crystals", and the Air Force Maui Optical Site.
Simplified multiple scattering model for radiative transfer in turbid water
NASA Technical Reports Server (NTRS)
Ghovanlou, A. H.; Gupta, G. N.
1978-01-01
Quantitative analytical procedures for relating selected water quality parameters to the characteristics of the backscattered signals, measured by remote sensors, require the solution of the radiative transport equation in turbid media. Presented is an approximate closed form solution of this equation and based on this solution, the remote sensing of sediments is discussed. The results are compared with other standard closed form solutions such as quasi-single scattering approximations.
The Hyperspectral Imager for the Coastal Ocean (HICO): Sensor and Data Processing Overview
2010-01-20
backscattering coefficients, and others. Several of these software modules will be developed within the Automated Processing System (APS), a data... Automated Processing System (APS) NRL developed APS, which processes satellite data into ocean color data products. APS is a collection of methods...used for ocean color processing which provide the tools for the automated processing of satellite imagery [1]. These tools are in the process of
NASA Astrophysics Data System (ADS)
Boreysho, Anatoly; Savin, Andrey; Morozov, Alexey; Konyaev, Maxim; Konovalov, Konstantin
2007-06-01
Recognition of aerosol clouds material at some significant distance is now a key requirement for the wide range of applications. The elastic backscatter lidar have demonstrated high capabilities in aerosol remote detection, cloud real-time mapping at very long distances for low-concentration natural aerosols as well as artificial ones [1]. However, recognition ability is required to make them more relevant. Laser-induced fluorescence (LIF) looks very promising with respect to the recognition problem. New approach based on mobile lidar complex [2] equipped by spectrally-and range-resolved LIF-sensor is described as well as some results of field tests. The LIF-sensor consists of four-harmonics Nd:YAG laser equipped by an output expander to provide final beam divergence <1 mrad, 500-mm aspheric Cassegrain-type multi-wavelength receiving telescope, set of single-element receivers for measurement of the elastic backscatter radiation, and multi-element receiver with monochromator for spectrally-resolved LIF measurements. The system is equipped by 2-axis scanning mirror and variable-FOV video-camera collimated with the lidar scanning direction. The LIF-lidar is mounted on a truck-based platform (20-feet container) as a part of multi-purpose mobile lidar complex and adjusted for field conditions.
Ribeiro, Rita S. R.; Cunha, João P. S.; Rosa, Carla C.; Jorge, Pedro A. S.
2018-01-01
Recent trends on microbiology point out the urge to develop optical micro-tools with multifunctionalities such as simultaneous manipulation and sensing. Considering that miniaturization has been recognized as one of the most important paradigms of emerging sensing biotechnologies, optical fiber tools, including Optical Fiber Tweezers (OFTs), are suitable candidates for developing multifunctional small sensors for Medicine and Biology. OFTs are flexible and versatile optotools based on fibers with one extremity patterned to form a micro-lens. These are able to focus laser beams and exert forces onto microparticles strong enough (piconewtons) to trap and manipulate them. In this paper, through an exploratory analysis of a 45 features set, including time and frequency-domain parameters of the back-scattered signal of particles trapped by a polymeric lens, we created a novel single feature able to differentiate synthetic particles (PMMA and Polystyrene) from living yeasts cells. This single statistical feature can be useful for the development of label-free hybrid optical fiber sensors with applications in infectious diseases detection or cells sorting. It can also contribute, by revealing the most significant information that can be extracted from the scattered signal, to the development of a simpler method for particles characterization (in terms of composition, heterogeneity degree) than existent technologies. PMID:29495502
Paiva, Joana S; Ribeiro, Rita S R; Cunha, João P S; Rosa, Carla C; Jorge, Pedro A S
2018-02-27
Recent trends on microbiology point out the urge to develop optical micro-tools with multifunctionalities such as simultaneous manipulation and sensing. Considering that miniaturization has been recognized as one of the most important paradigms of emerging sensing biotechnologies, optical fiber tools, including Optical Fiber Tweezers (OFTs), are suitable candidates for developing multifunctional small sensors for Medicine and Biology. OFTs are flexible and versatile optotools based on fibers with one extremity patterned to form a micro-lens. These are able to focus laser beams and exert forces onto microparticles strong enough (piconewtons) to trap and manipulate them. In this paper, through an exploratory analysis of a 45 features set, including time and frequency-domain parameters of the back-scattered signal of particles trapped by a polymeric lens, we created a novel single feature able to differentiate synthetic particles (PMMA and Polystyrene) from living yeasts cells. This single statistical feature can be useful for the development of label-free hybrid optical fiber sensors with applications in infectious diseases detection or cells sorting. It can also contribute, by revealing the most significant information that can be extracted from the scattered signal, to the development of a simpler method for particles characterization (in terms of composition, heterogeneity degree) than existent technologies.
NASA Technical Reports Server (NTRS)
Kaupp, V. H.; Macdonald, H. C.; Waite, W. P.
1981-01-01
The initial phase of a program to determine the best interpretation strategy and sensor configuration for a radar remote sensing system for geologic applications is discussed. In this phase, terrain modeling and radar image simulation were used to perform parametric sensitivity studies. A relatively simple computer-generated terrain model is presented, and the data base, backscatter file, and transfer function for digital image simulation are described. Sets of images are presented that simulate the results obtained with an X-band radar from an altitude of 800 km and at three different terrain-illumination angles. The simulations include power maps, slant-range images, ground-range images, and ground-range images with statistical noise incorporated. It is concluded that digital image simulation and computer modeling provide cost-effective methods for evaluating terrain variations and sensor parameter changes, for predicting results, and for defining optimum sensor parameters.
Xu, Jialin; Donepudi, Ajay C; More, Vijay R; Kulkarni, Supriya R; Li, Liya; Guo, Liangran; Yan, Bingfang; Chatterjee, Tapan; Weintraub, Neal; Slitt, Angela L
2015-02-01
To evaluate whether Nrf2 deficiency impacts insulin resistance and lipid accumulation in liver and white adipose tissue. Lep(ob/ob) mice (OB) with targeted Nrf2 deletion (OB-Nrf2KO) were generated. Pathogenesis of obesity and type 2 diabetes was measured in C57BL/6J, Nrf2KO, OB, and OB-Nrf2KO mice. Hepatic lipid content, lipid clearance, and very low-density lipoprotein (VLDL) secretion were determined between OB and OB-Nrf2KO mice. OB-Nrf2KO mice exhibited decreased white adipose tissue mass and decreased adipogenic and lipogenic gene expression compared with OB mice. Nrf2 deficiency prolonged hyperglycemia in response to glucose challenge, which was paralleled by reduced insulin-stimulated Akt phosphorylation. In OB mice, Nrf2 deficiency decreased hepatic lipid accumulation, decreased peroxisome proliferator-activated receptor γ expression and nicotinamide adenine dinucleotide phosphate (NADPH) content, and enhanced VLDL secretion. However, this observation was opposite in lean mice. Additionally, OB-Nrf2KO mice exhibited increased plasma triglyceride content, decreased HDL-cholesterol content, and enhanced apolipoprotein B expression, suggesting Nrf2 deficiency caused dyslipidemia in these mice. Nrf2 deficiency in Lep(ob/ob) mice reduced white adipose tissue mass and prevented hepatic lipid accumulation but induced insulin resistance and dyslipidemia. This study indicates a dual role of Nrf2 during metabolic dysregulation-increasing lipid accumulation in liver and white adipose tissue but preventing lipid accumulation in obese mice. © 2014 The Obesity Society.
Cool, Barbara; Zinker, Bradley; Chiou, William; Kifle, Lemma; Cao, Ning; Perham, Matthew; Dickinson, Robert; Adler, Andrew; Gagne, Gerard; Iyengar, Rajesh; Zhao, Gang; Marsh, Kennan; Kym, Philip; Jung, Paul; Camp, Heidi S; Frevert, Ernst
2006-06-01
AMP-activated protein kinase (AMPK) is a key sensor and regulator of intracellular and whole-body energy metabolism. We have identified a thienopyridone family of AMPK activators. A-769662 directly stimulated partially purified rat liver AMPK (EC50 = 0.8 microM) and inhibited fatty acid synthesis in primary rat hepatocytes (IC50 = 3.2 microM). Short-term treatment of normal Sprague Dawley rats with A-769662 decreased liver malonyl CoA levels and the respiratory exchange ratio, VCO2/VO2, indicating an increased rate of whole-body fatty acid oxidation. Treatment of ob/ob mice with 30 mg/kg b.i.d. A-769662 decreased hepatic expression of PEPCK, G6Pase, and FAS, lowered plasma glucose by 40%, reduced body weight gain and significantly decreased both plasma and liver triglyceride levels. These results demonstrate that small molecule-mediated activation of AMPK in vivo is feasible and represents a promising approach for the treatment of type 2 diabetes and the metabolic syndrome.
Chromium (D-phenylalanine)3 improves obesity-induced cardiac contractile defect in ob/ob mice.
Dong, Feng; Yang, Xiaoping; Sreejayan, Nair; Ren, Jun
2007-11-01
Low-molecular weight chromium compounds, such as chromium picolinate [Cr(pic)(3)], improve insulin sensitivity, although toxicity is a concern. We synthesized a novel chromium complex, chromium (d-phenylalanine)(3) [Cr(d-phe)(3)], in an attempt to improve insulin sensitivity with reduced toxicity. The aim of this study was to compare the two chromium compounds on cardiac contractile function in ob/ob obese mice. C57BL lean and ob/ob obese mice were randomly divided into three groups: H(2)O, Cr(d-phe)(3), or Cr(pic)(3) (45 mug/kg per day orally for 6 months). The glucose tolerance test displayed improved glucose clearance by Cr(d-phe)(3) but not Cr(pic)(3). Myocytes from ob/ob mice exhibited depressed peak shortening (PS) and maximal velocity of shortening/relengthening (+/-dL/dt), prolonged time-to-PS and time-to-90% relengthening (TR90), reduced electrically stimulated rise in intracellular Ca(2+) (Deltafura-2 fluorescence intensity), and slowed intracellular Ca(2+) decay. Although a 3-month Cr(d-phe)(3) treatment for a separate group of ob/ob and lean 2-month-old mice only rectified reduced +/-dL/dt in ob/ob mice, all mechanical and intracellular Ca(2+) abnormalities were significantly attenuated or ablated by 6 months of Cr(d-phe)(3) but not Cr(pic)(3) treatment (except TR90). Sarco(endo)plasmic reticulum Ca(2+) ATPase activity and Na(+)-Ca(2+) exchanger expression were depressed in ob/ob mice, which were reversed by both Cr(d-phe)(3) and Cr(pic)(3), with a more pronounced effect from Cr(d-phe)(3). Cr(d-phe)(3) corrected reduced insulin-stimulated glucose uptake and improved basal phosphorylation of Akt and insulin receptor, as well as insulin-stimulated phosphorylation of Akt and insulin receptor in ob/ob myocytes. Heart homogenates from ob/ob mice had enhanced oxidative stress and protein carbonyl formation compared with the lean group, which were attenuated by both Cr(d-phe)(3) and Cr(pic)(3). Our data suggest that the new Cr(d-phe)(3) compound possesses better cardio-protective and insulin-sensitizing properties against obesity.
Brain volume in early MS patients with and without IgG oligoclonal bands in CSF.
Fenu, G; Lorefice, L; Sechi, V; Loi, L; Contu, F; Cabras, F; Coghe, G; Frau, J; Secci, M A; Melis, C; Schirru, L; Costa, G; Melas, V; Arru, M; Barracciu, M A; Marrosu, M G; Cocco, E
2018-01-01
Oligoclonal bands of IgG (OB) are proposed as an early prognostic factor of the disease. Growing attention is directed towards brain volume evaluation as a possible marker of the severity of MS. Previous studies found that MS patients lacking OB have less brain atrophy. to evaluate a possible relationship between OB and cerebral volume in a cohort of early MS patients. Inclusion criteria were: diagnosis of relapsing-remitting MS; CSF analysis and MRI acquired simultaneously and within 12 months from clinical onset. A total of 15 healthy controls underwent MRI. In 20 MS patients, CSF analysis did not show OB synthesis (OB negative group). A control group of 25 MS patients in whom OB was detected was also randomly recruited (OB positive group). T test showed a significant difference in NWV between the OB positive and OB negative groups (P value = 0.01), and between the OB positive group and the healthy controls (P value = 0.001). No differences were detected between OB negative group and healthy controls. Multivariable linear regression showed a relationship between NWV and OB synthesis (P value = 0.02) controlling for age, gender, and EDSS. Our preliminary results suggest that OB positive patients show more atrophy of white matter since early phases of the disease, supporting the role of CSF analysis as a prognostic factor in MS. Copyright © 2017 Elsevier B.V. All rights reserved.
Switching performance of OBS network model under prefetched real traffic
NASA Astrophysics Data System (ADS)
Huang, Zhenhua; Xu, Du; Lei, Wen
2005-11-01
Optical Burst Switching (OBS) [1] is now widely considered as an efficient switching technique in building the next generation optical Internet .So it's very important to precisely evaluate the performance of the OBS network model. The performance of the OBS network model is variable in different condition, but the most important thing is that how it works under real traffic load. In the traditional simulation models, uniform traffics are usually generated by simulation software to imitate the data source of the edge node in the OBS network model, and through which the performance of the OBS network is evaluated. Unfortunately, without being simulated by real traffic, the traditional simulation models have several problems and their results are doubtable. To deal with this problem, we present a new simulation model for analysis and performance evaluation of the OBS network, which uses prefetched IP traffic to be data source of the OBS network model. The prefetched IP traffic can be considered as real IP source of the OBS edge node and the OBS network model has the same clock rate with a real OBS system. So it's easy to conclude that this model is closer to the real OBS system than the traditional ones. The simulation results also indicate that this model is more accurate to evaluate the performance of the OBS network system and the results of this model are closer to the actual situation.
NASA Astrophysics Data System (ADS)
Sierra-Pérez, Julián; Torres-Arredondo, M.-A.; Alvarez-Montoya, Joham
2018-01-01
Structural health monitoring consists of using sensors integrated within structures together with algorithms to perform load monitoring, damage detection, damage location, damage size and severity, and prognosis. One possibility is to use strain sensors to infer structural integrity by comparing patterns in the strain field between the pristine and damaged conditions. In previous works, the authors have demonstrated that it is possible to detect small defects based on strain field pattern recognition by using robust machine learning techniques. They have focused on methodologies based on principal component analysis (PCA) and on the development of several unfolding and standardization techniques, which allow dealing with multiple load conditions. However, before a real implementation of this approach in engineering structures, changes in the strain field due to conditions different from damage occurrence need to be isolated. Since load conditions may vary in most engineering structures and promote significant changes in the strain field, it is necessary to implement novel techniques for uncoupling such changes from those produced by damage occurrence. A damage detection methodology based on optimal baseline selection (OBS) by means of clustering techniques is presented. The methodology includes the use of hierarchical nonlinear PCA as a nonlinear modeling technique in conjunction with Q and nonlinear-T 2 damage indices. The methodology is experimentally validated using strain measurements obtained by 32 fiber Bragg grating sensors bonded to an aluminum beam under dynamic bending loads and simultaneously submitted to variations in its pitch angle. The results demonstrated the capability of the methodology for clustering data according to 13 different load conditions (pitch angles), performing the OBS and detecting six different damages induced in a cumulative way. The proposed methodology showed a true positive rate of 100% and a false positive rate of 1.28% for a 99% of confidence.
NASA Astrophysics Data System (ADS)
Werth, Alexandra; Liakat, Sabbir; Dong, Anqi; Woods, Callie M.; Gmachl, Claire F.
2018-05-01
An integrating sphere is used to enhance the collection of backscattered light in a noninvasive glucose sensor based on quantum cascade laser spectroscopy. The sphere enhances signal stability by roughly an order of magnitude, allowing us to use a thermoelectrically (TE) cooled detector while maintaining comparable glucose prediction accuracy levels. Using a smaller TE-cooled detector reduces form factor, creating a mobile sensor. Principal component analysis has predicted principal components of spectra taken from human subjects that closely match the absorption peaks of glucose. These principal components are used as regressors in a linear regression algorithm to make glucose concentration predictions, over 75% of which are clinically accurate.
Top/bottom multisensor remote sensing of Arctic sea ice
NASA Technical Reports Server (NTRS)
Comiso, J. C.; Wadhams, P.; Krabill, W. B.; Swift, R. N.; Crawford, J. P.
1991-01-01
Results are presented on the Aircraft/Submarine Sea Ice Project experiment carried out in May 1987 to investigate concurrently the top and the bottom features of the Arctic sea-ice cover. Data were collected nearly simultaneously by instruments aboard two aircraft and a submarine, which included passive and active (SAR) microwave sensors, upward looking and sidescan sonars, a lidar profilometer, and an IR sensor. The results described fall into two classes of correlations: (1) quantitative correlations between profiles, such as ice draft (sonar), ice elevation (laser), SAR backscatter along the track line, and passive microwave brightness temperatures; and (2) qualitative and semiquantitative correlations between corresponding areas of imagery (i.e., passive microwave, AR, and sidescan sonar).
NASA Astrophysics Data System (ADS)
Yi, Xiaohua; Cho, Chunhee; Cooper, James; Wang, Yang; Tentzeris, Manos M.; Leon, Roberto T.
2013-08-01
This research investigates a passive wireless antenna sensor designed for strain and crack sensing. When the antenna experiences deformation, the antenna shape changes, causing a shift in the electromagnetic resonance frequency of the antenna. A radio frequency identification (RFID) chip is adopted for antenna signal modulation, so that a wireless reader can easily distinguish the backscattered sensor signal from unwanted environmental reflections. The RFID chip captures its operating power from an interrogation electromagnetic wave emitted by the reader, which allows the antenna sensor to be passive (battery-free). This paper first reports the latest simulation results on radiation patterns, surface current density, and electromagnetic field distribution. The simulation results are followed with experimental results on the strain and crack sensing performance of the antenna sensor. Tensile tests show that the wireless antenna sensor can detect small strain changes lower than 20 με, and can perform well at large strains higher than 10 000 με. With a high-gain reader antenna, the wireless interrogation distance can be increased up to 2.1 m. Furthermore, an array of antenna sensors is capable of measuring the strain distribution in close proximity. During emulated crack and fatigue crack tests, the antenna sensor is able to detect the growth of a small crack.
Empirical algorithms to estimate water column pH in the Southern Ocean
NASA Astrophysics Data System (ADS)
Williams, N. L.; Juranek, L. W.; Johnson, K. S.; Feely, R. A.; Riser, S. C.; Talley, L. D.; Russell, J. L.; Sarmiento, J. L.; Wanninkhof, R.
2016-04-01
Empirical algorithms are developed using high-quality GO-SHIP hydrographic measurements of commonly measured parameters (temperature, salinity, pressure, nitrate, and oxygen) that estimate pH in the Pacific sector of the Southern Ocean. The coefficients of determination, R2, are 0.98 for pH from nitrate (pHN) and 0.97 for pH from oxygen (pHOx) with RMS errors of 0.010 and 0.008, respectively. These algorithms are applied to Southern Ocean Carbon and Climate Observations and Modeling (SOCCOM) biogeochemical profiling floats, which include novel sensors (pH, nitrate, oxygen, fluorescence, and backscatter). These algorithms are used to estimate pH on floats with no pH sensors and to validate and adjust pH sensor data from floats with pH sensors. The adjusted float data provide, for the first time, seasonal cycles in surface pH on weekly resolution that range from 0.05 to 0.08 on weekly resolution for the Pacific sector of the Southern Ocean.
Kemp, J G; Blazev, R; Stephenson, D G; Stephenson, G M M
2009-08-01
Knowledge of the morphological and biochemical alterations occurring in skeletal muscles of obese animals is relatively limited, particularly with respect to non-limb muscles and relationship to fibre type. Sternomastoid (SM; fast-twitch), extensor digitorum longus (EDL; fast-twitch), and soleus (SOL; mixed) muscles of ob/ob mouse (18-22 weeks) were examined with respect to size (mass, muscle mass-to-body mass ratio, cross-sectional area (CSA)), fibre CSA, protein content, myosin heavy chain (MHC) content, MHC isoform (MHC(i)) composition, MHC(i)-based fibre type composition, and lactate dehydrogenase isoenzyme (LDH(iso)) composition. Compared with (control) muscles from lean mice, all the three muscles from ob/ob mice were smaller in size (by 13-30%), with SM and EDL being the most affected. The CSA of IIB and IIB+IID fibres (the predominant fibre types in SM and EDL muscles) was markedly smaller (by approximately 30%) in ob/ob mice, consistent with differences in muscle size. Total protein content (normalised to muscle mass) was significantly lower in EDL (-9.7%) and SOL (-14.1%) muscles of ob/ob mice, but there were no differences between SM, EDL, and SOL muscles from the two animal groups with respect to MHC content (also normalised to muscle mass). Electrophoretic analyses of MHC(i) composition in whole muscle homogenates and single muscle fibres showed a shift towards slower MHC(i) content, slower MHC(i) containing fibres, and a greater proportion of hybrid fibres in all the three muscles of ob/ob mice, with a shift towards a more aerobic-oxidative phenotype also observed with respect to LDH(iso) composition. This study showed that SM, EDL, and SOL muscles of ob/ob mice display size reductions to an extent that seems to be largely related to fibre type composition, and a shift in fibre type composition that may result from a process of structural remodelling, as suggested by the increased proportion of hybrid fibres in muscles of ob/ob mice.
Manolescu, Daniel-Constantin; Jankowski, Marek; Danalache, Bogdan A; Wang, Donghao; Broderick, Tom L; Chiasson, Jean-Louis; Gutkowska, Jolanta
2014-10-01
In hypertensive rodents, retinoic acid (RA) prevents adverse cardiac remodelling and improves myocardial infarction outcome, but its role in obesity-related changes of cardiac tissue are unclear. We hypothesized that all-trans RA (ATRA) treatment will improve the cardioprotective oxytocin-natriuretic peptides (OT-NP) system, preventing apoptosis and collagen accumulation in hearts of ob/ob mice, a mouse model of obesity and insulin resistance. Female 9-week-old B6.V-Lep/J ob/ob mice (n = 16) were divided into 2 groups: 1 group (n = 8) treated with 100 μg of ATRA dissolved in 100 μL of corn oil (vehicle) delivered daily (∼2 μg·g body weight(-1)·day(-1)) by stomach intubation for 16 days, and 1 group (n = 8) that received the vehicle alone. A group of nonobese littermate mice (n = 9) served as controls. Ob/ob mice exhibited obesity, hyperglycaemia, and downregulation of the cardiac OT-NP system, including the mRNA for the transcription factor GATA4, OT receptor and brain NP, and the protein expression for endothelial nitric oxide synthase. Hearts from ob/ob mice also demonstrated increased apoptosis and collagen accumulation. ATRA treatment induced weight loss and decreased adipocytes diameter in the visceral fat, thus reducing visceral obesity, which is associated with a high risk for cardiovascular disease. RA treatment was associated with a reduction in hyperglycemia and a normalization of the OT-NP system's expression in the hearts of ob/ob mice. Furthermore, ATRA treatment prevented apoptosis and collagen accumulation in hearts of ob/ob mice. The present study indicates that ATRA treatment was effective in restoring the cardioprotective OT-NP system and in preventing abnormal cardiac remodelling in the ob/ob mice.
Current status of visibility sensors for aviation
NASA Technical Reports Server (NTRS)
Burnham, D. C.
1983-01-01
The development of a new increased range higher visibility was investigated. The visibility sensor that is currently is the Transmissometer. Current transmissometer technology provides only a factor of 10 dynamic range with a single base line. The FAA is preparing to install automated weather observing systems (AWOS) at many locations. These systems require visibilities up to approximately 5 miles. To use a transmissometer for this type of measurement, the base line must be about 1,000 feet; where alignment becomes very difficult to maintain. New technologies were developd for measuring visibility. Improved transmissometers, forward-scatter meters and back-scatter meters have become available. A current practical issue for visibility sensors is how to specify one that is good enough to meet the needs of aviation. The first question is what performance is required; how accurately must the sensor measure? Visibility sensors do not actually measure the visibility directly. The purpose of measuring the visibility is to predict what the pilot will see a considerable distance away from the sensor location. The atmosphere introduces considerable variation in the measurement and the basic sensor accuracy needed is difficult to define. The second question for high visibilities is what to use as the standard reference sensor. The third question pertains to the competitive procurement of visibility sensors, which is mandated at present. Two acceptance test procedures to be used to insure satisfactory sensor performance is examined.
Takayama, Yukihisa; Nishie, Akihiro; Asayama, Yoshiki; Ishigami, Kousei; Kakihara, Daisuke; Ushijima, Yasuhiro; Fujita, Nobuhiro; Shirabe, Ken; Takemura, Atsushi; Honda, Hiroshi
2017-05-18
To retrospectively evaluate the diagnostic performance of free-breathing diffusion-weighted imaging (FB-DWI) with modified imaging parameter settings for detecting hepatocellular carcinomas (HCCs). Fifty-one patients at risk for HCC were scanned with both FB-DWI and respiratory-triggered DWI with the navigator echo respiratory-triggering technique (RT-DWI). Qualitatively, the sharpness of the liver contour, the image noise and the chemical shift artifacts on each DWI with b -values of 1000 s/mm 2 were independently evaluated by three radiologists using 4-point scoring. We compared the image quality scores of each observer between the two DWI methods, using the Wilcoxon signed-rank test. Quantitatively, we compared the signal-to-noise ratios (SNRs) of the liver parenchyma and lesion-to-nonlesion contrast-to-noise ratios (CNRs) after measuring the signal intensity on each DWI with a b-factor of 1000 s/mm 2 . The average SNRs and CNRs between the two DWI methods were compared by the paired t-test. The detectability of HCC on each DWI was also analyzed by three radiologists. The detectability provided by the two DWI methods was compared using McNemar's test. For all observers, the averaged image quality scores of FB-DWI were: Sharpness of the liver contour [observer (Obs)-1, 3.08 ± 0.81; Obs-2, 2.98 ± 0.73; Obs-3, 3.54 ± 0.75], those of the distortion (Obs-1, 2.94 ± 0.50; Obs-2, 2.71 ± 0.70; Obs-3, 3.27 ± 0.53), and the chemical shift artifacts (Obs-1, 3.38 ± 0.60; Obs-2, 3.15 ± 1.07; Obs-3, 3.21 ± 0.85). The averaged image quality scores of RT-DWI were: Sharpness of the liver contour (Obs-1, 2.33 ± 0.65; Obs-2, 2.37 ± 0.74; Obs-3, 2.75 ± 0.81), distortion (Obs-1, 2.81 ± 0.56; Obs-2, 2.25 ± 0.74; Obs-3, 2.96 ± 0.71), and the chemical shift artifacts (Obs-1, 2.92 ± 0.59; Obs-2, 2.21 ± 0.85; Obs-3, 2.77 ± 1.08). All image quality scores of FB-DWI were significantly higher than those of RT-DWI ( P < 0.05). The average SNR of the normal liver parenchyma by FB-DWI (11.0 ± 4.8) was not significantly different from that shown by RT-DWI (11.0 ± 5.0); nor were the lesion-to-nonlesion CNRs significantly different (FB-DWI, 21.4 ± 17.7; RT-DWI, 20.1 ± 15.1). For all three observers, the detectability of FB-DWI (Obs-1, 43.6%; Obs-2, 53.6%; and Obs-3, 45.0%) was significantly higher than that of RT-DWI (Obs-1, 29.1%; Obs-2, 43.6%; and Obs-3, 34.5%) ( P < 0.05). FB-DWI showed better image quality and higher detectability of HCC compared to RT-DWI, without significantly reducing the SNRs of the liver parenchyma and lesion-to-nonlesion CNRs.
Takayama, Yukihisa; Nishie, Akihiro; Asayama, Yoshiki; Ishigami, Kousei; Kakihara, Daisuke; Ushijima, Yasuhiro; Fujita, Nobuhiro; Shirabe, Ken; Takemura, Atsushi; Honda, Hiroshi
2017-01-01
AIM To retrospectively evaluate the diagnostic performance of free-breathing diffusion-weighted imaging (FB-DWI) with modified imaging parameter settings for detecting hepatocellular carcinomas (HCCs). METHODS Fifty-one patients at risk for HCC were scanned with both FB-DWI and respiratory-triggered DWI with the navigator echo respiratory-triggering technique (RT-DWI). Qualitatively, the sharpness of the liver contour, the image noise and the chemical shift artifacts on each DWI with b-values of 1000 s/mm2 were independently evaluated by three radiologists using 4-point scoring. We compared the image quality scores of each observer between the two DWI methods, using the Wilcoxon signed-rank test. Quantitatively, we compared the signal-to-noise ratios (SNRs) of the liver parenchyma and lesion-to-nonlesion contrast-to-noise ratios (CNRs) after measuring the signal intensity on each DWI with a b-factor of 1000 s/mm2. The average SNRs and CNRs between the two DWI methods were compared by the paired t-test. The detectability of HCC on each DWI was also analyzed by three radiologists. The detectability provided by the two DWI methods was compared using McNemar’s test. RESULTS For all observers, the averaged image quality scores of FB-DWI were: Sharpness of the liver contour [observer (Obs)-1, 3.08 ± 0.81; Obs-2, 2.98 ± 0.73; Obs-3, 3.54 ± 0.75], those of the distortion (Obs-1, 2.94 ± 0.50; Obs-2, 2.71 ± 0.70; Obs-3, 3.27 ± 0.53), and the chemical shift artifacts (Obs-1, 3.38 ± 0.60; Obs-2, 3.15 ± 1.07; Obs-3, 3.21 ± 0.85). The averaged image quality scores of RT-DWI were: Sharpness of the liver contour (Obs-1, 2.33 ± 0.65; Obs-2, 2.37 ± 0.74; Obs-3, 2.75 ± 0.81), distortion (Obs-1, 2.81 ± 0.56; Obs-2, 2.25 ± 0.74; Obs-3, 2.96 ± 0.71), and the chemical shift artifacts (Obs-1, 2.92 ± 0.59; Obs-2, 2.21 ± 0.85; Obs-3, 2.77 ± 1.08). All image quality scores of FB-DWI were significantly higher than those of RT-DWI (P < 0.05). The average SNR of the normal liver parenchyma by FB-DWI (11.0 ± 4.8) was not significantly different from that shown by RT-DWI (11.0 ± 5.0); nor were the lesion-to-nonlesion CNRs significantly different (FB-DWI, 21.4 ± 17.7; RT-DWI, 20.1 ± 15.1). For all three observers, the detectability of FB-DWI (Obs-1, 43.6%; Obs-2, 53.6%; and Obs-3, 45.0%) was significantly higher than that of RT-DWI (Obs-1, 29.1%; Obs-2, 43.6%; and Obs-3, 34.5%) (P < 0.05). CONCLUSION FB-DWI showed better image quality and higher detectability of HCC compared to RT-DWI, without significantly reducing the SNRs of the liver parenchyma and lesion-to-nonlesion CNRs. PMID:28588750
Design data collection with Skylab microwave radiometer-scatterometer S-193, volume 2
NASA Technical Reports Server (NTRS)
Moore, R. K.; Ulaby, F. T. (Principal Investigator)
1975-01-01
The author has identified the following significant results. Skylab S-193 radiometer/scatterometer produced terrain responses with various polarizations and observation angles for cells of 100 to 400 sq km area. Classification of the observations into natural categories was achieved by K-means and spatial clustering algorithms. Microwave data acquired over the Great Salt Lake Desert area by sensors aboard Skylab and Nimbus 5 indicate that the microwave emission and backscatter were strongly influenced by contributions from subsurface layers of sediment saturated with brine. Correlations were noted between microwave backscatter response at approximately 33 deg from scatterometer (operating at 13.9 GHz) and the configuration of ground targets in Brazil as discerned from coarse scale maps. With limited, available ground truth, these correlations were sufficient to permit the production of image-like displays which bear a marked resemblance to known terrain features in several instances.
Characteristics of 13.9 GHz radar scattering from oil films on the sea surface
NASA Technical Reports Server (NTRS)
Johnson, J. W.; Croswell, W. F.
1982-01-01
Aircraft microwave scatterometer measurements are presented, which were made in 1979 as part of a project to study the response of a number of active and passive microwave and optical remote sensors to an oil-covered sea surface conducted by NASA Langley Research Center. A 13.9-GHz Doppler scatterometer with a fan beam antenna and coherent detection was used to measure radar backscatter as a function of incidence angle. The radar scattering signature of the clear surface and signatures of the surface covered with various crude oil films are compared. Reductions in Ku band microwave backscatter up to 14 dB are observed for both treated and untreated LaRosa and Murban crude oil films deposited on the sea surface. Maximum Ku band sensitivity to the effects of the oil in terms of differential scatter is observed in the 25-35 deg incidence angle region.
Schaab, Michael; Kratzsch, Juergen
2015-10-01
The adipokine leptin realizes signal transduction via four different leptin receptor (OB-R) isoforms. The amount of functionally active OB-R, however, is affected by constitutive shedding of the extracellular domain. The product of the cleavage process, the so-called soluble leptin receptor (sOB-R), is the main binding protein for leptin in human blood and modulates its bioavailability. Concentrations of sOB-R are differentially regulated in metabolic disorders, such as type 1 diabetes mellitus or obesity, and can, therefore, enhance or reduce leptin sensitivity. Lipotoxicity and apoptosis increase OB-R cleavage via ADAM10-dependent mechanisms. In contrast, although increased sOB-R concentrations seem to directly inhibit leptin effects, reduced amounts of sOB-R may reflect decreased membrane expression of OB-R. These findings, in part, explain alterations of leptin sensitivity that are associated with changes in serum sOB-R concentrations seen in metabolic disorders. Copyright © 2015 Elsevier Ltd. All rights reserved.
Leptin receptor mRNA in rat brain astrocytes
Hsuchou, Hung; Pan, Weihong; Barnes, Maria J.; Kastin, Abba J.
2009-01-01
We recently reported that mouse astrocytes express leptin receptors (ObR), and that obesity induces upregulation of astrocytic ObR. To provide further evidence of the importance of astrocytic ObR expression, we performed double-labeling fluorescent in-situ hybridization (FISH) and immunohistochemistry in the rat hypothalamus. Laser confocal microscopic image analysis showed that ObR mRNA was present in glial fibrillary acidic protein (+) cells that show distinctive astrocytic morphology as well as in neurons. In addition to the presence of ObR mRNA, ObR protein was shown in both astrocytes and neurons in the rat hypothalamus by double-labeling immunohistochemistry. In cultured rat C6 astrocytoma cells treated with different doses of lipopolysaccharide for 6 h, the mRNA for ObRa or ObRb did not show significant changes, as measured by quantitative RT-PCR. However, the protein expression of both ObRa and ObRb, determined by western blotting, was increased after the C6 cells were treated with either lipopolysaccharide or tumor necrosis factor-α. The results indicate that astrocytic ObR expression is present in rats as well as mice, and that it probably plays a role in the neuroinflammatory response. PMID:19747514
Radar Measurements of Ocean Surface Waves using Proper Orthogonal Decomposition
2017-03-30
rely on use of Fourier transforms (FFT) and filtering spectra on the linear dispersion relationship for ocean surface waves. This report discusses...the measured signal (e.g., Young et al., 1985). In addition, the methods often rely on filtering the FFT of radar backscatter or Doppler velocities...to those obtained with conventional FFT and dispersion curve filtering techniques (iv) Compare both results of(iii) to ground truth sensors (i .e
Fromenty, Bernard; Vadrot, Nathalie; Massart, Julie; Turlin, Bruno; Barri-Ova, Nadège; Lettéron, Philippe; Fautrel, Alain; Robin, Marie-Anne
2009-10-01
Clinical studies suggest that moderate alcohol consumption can have beneficial effects, in particular regarding cardiovascular events, insulin resistance, and type 2 diabetes. In this study, lean and obese diabetic ob/ob mice were submitted or not to chronic ethanol intake via the drinking water for 6 months, which was associated with moderate levels of plasma ethanol. Plasma levels of alanine aminotransferase and aspartate aminotransferase were not increased by alcohol intake. Ethanol consumption progressively reduced the gain of body weight in ob/ob mice, but not in lean mice, and this was observed despite higher calorie intake. Increased plasma free fatty acids and glycerol in ethanol-treated ob/ob mice suggested peripheral lipolysis. Glycemia and insulinemia were significantly reduced, whereas adiponectinemia was increased in ethanol-treated ob/ob mice. Liver weight and triglycerides were significantly decreased in ethanol-treated ob/ob mice, and this was associated with less microvesicular steatosis. Hepatic levels of AMP-activated protein kinase and the phosphorylated form of acetyl-CoA carboxylase were higher in ethanol-treated ob/ob mice, suggesting better fatty acid oxidation. However, hepatic mRNA expression of several lipogenic genes was not reduced by ethanol consumption. Finally, mild oxidative stress was noticed in the liver of ethanol-treated mice, regardless of their genotype. Hence, our data are in keeping with clinical studies suggesting that moderate ethanol intake can have beneficial effects on type 2 diabetes and insulin sensitivity, at least in part through increased levels of plasma adiponectin. However, further studies are needed to determine whether long-term drinking of light-to-moderate amounts of ethanol is safe for the liver.
Noel, Camille E; Parikh, Parag J; Spencer, Christopher R; Green, Olga L; Hu, Yanle; Mutic, Sasa; Olsen, Jeffrey R
2015-01-01
Onboard magnetic resonance imaging (OB-MRI) for daily localization and adaptive radiotherapy has been under development by several groups. However, no clinical studies have evaluated whether OB-MRI improves visualization of the target and organs at risk (OARs) compared to standard onboard computed tomography (OB-CT). This study compared visualization of patient anatomy on images acquired on the MRI-(60)Co ViewRay system to those acquired with OB-CT. Fourteen patients enrolled on a protocol approved by the Institutional Review Board (IRB) and undergoing image-guided radiotherapy for cancer in the thorax (n = 2), pelvis (n = 6), abdomen (n = 3) or head and neck (n = 3) were imaged with OB-MRI and OB-CT. For each of the 14 patients, the OB-MRI and OB-CT datasets were displayed side-by-side and independently reviewed by three radiation oncologists. Each physician was asked to evaluate which dataset offered better visualization of the target and OARs. A quantitative contouring study was performed on two abdominal patients to assess if OB-MRI could offer improved inter-observer segmentation agreement for adaptive planning. In total 221 OARs and 10 targets were compared for visualization on OB-MRI and OB-CT by each of the three physicians. The majority of physicians (two or more) evaluated visualization on MRI as better for 71% of structures, worse for 10% of structures, and equivalent for 14% of structures. 5% of structures were not visible on either. Physicians agreed unanimously for 74% and in majority for > 99% of structures. Targets were better visualized on MRI in 4/10 cases, and never on OB-CT. Low-field MR provides better anatomic visualization of many radiotherapy targets and most OARs as compared to OB-CT. Further studies with OB-MRI should be pursued.
Hydrogen sulfide improves diabetic wound healing in ob/ob mice via attenuating inflammation.
Zhao, Huichen; Lu, Shengxia; Chai, Jiachao; Zhang, Yuchao; Ma, Xiaoli; Chen, Jicui; Guan, Qingbo; Wan, Meiyan; Liu, Yuantao
2017-09-01
The proposed mechanisms of impaired wound healing in diabetes involve sustained inflammation, excess oxidative stress and compromised agiogenesis. Hydrogen sulfide (H 2 S) has been reported to have multiple biological activities. We aim to investigate the role of H 2 S in impaired wound healing in ob/ob mice and explore the possible mechanisms involved. Full-thickness skin dorsal wounds were created on ob/ob mice and C57BL/6 mice. Cystathionine-γ-lyase (CSE) expression and H 2 S production were determined in granulation tissues of the wounds. Effects of NaHS on wound healing were evaluated. Inflammation and angiogenesis in granulation tissues of the wounds were examined. CSE expression, and H 2 S content were significantly reduced in granulation tissues of wounds in ob/ob mice compared with control mice. NaHS treatment significantly improved wound healing in ob/ob mice, which was associated with reduced neutrophil and macrophage infiltration, decreased production of tumor necrosis factor (TNF)-α, interleukin (IL)-6. NaHS treatment decreased metalloproteinase (MMP)-9, whereas increased collagen deposition and vascular-like structures in granulation tissues of wounds in ob/ob mice. CSE down-regulation may play a role in the pathogenesis of diabetic impaired wound healing. Exogenous H 2 S could be a potential agent to improve diabetic impaired wound healing by attenuating inflammation and increasing angiogenesis. Copyright © 2017 Elsevier Inc. All rights reserved.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Inami, Yoshihiro; Yamashina, Shunhei, E-mail: syamashi@juntendo.ac.jp; Izumi, Kousuke
2011-09-09
Highlights: {yields} Acidification of autophagosome was blunted in steatotic hepatocytes. {yields} Hepatic steatosis did not disturb fusion of isolated autophagosome and lysosome. {yields} Proteinase activity of cathepsin B and L in autolysosomes was inhibited by steatosis. {yields} Hepatic expression of cathepsin B and L was suppressed by steatosis. -- Abstract: Autophagy, one of protein degradation system, contributes to maintain cellular homeostasis and cell defense. Recently, some evidences indicated that autophagy and lipid metabolism are interrelated. Here, we demonstrate that hepatic steatosis impairs autophagic proteolysis. Though accumulation of autophagosome is observed in hepatocytes from ob/ob mice, expression of p62 was augmentedmore » in liver from ob/ob mice more than control mice. Moreover, degradation of the long-lived protein leucine was significantly suppressed in hepatocytes isolated from ob/ob mice. More than 80% of autophagosomes were stained by LysoTracker Red (LTR) in hepatocytes from control mice; however, rate of LTR-stained autophagosomes in hepatocytes were suppressed in ob/ob mice. On the other hand, clearance of autolysosomes loaded with LTR was blunted in hepatocytes from ob/ob mice. Although fusion of isolated autophagosome and lysosome was not disturbed, proteinase activity of cathepsin B and L in autolysosomes and cathepsin B and L expression of liver were suppressed in ob/ob mice. These results indicate that lipid accumulation blunts autophagic proteolysis via impairment of autophagosomal acidification and cathepsin expression.« less
Proteomic analysis of oil bodies in mature Jatropha curcas seeds with different lipid content.
Liu, Hui; Wang, Cuiping; Chen, Fan; Shen, Shihua
2015-01-15
To reveal the difference among three mature Jatropha curcas seeds (JcVH, variant with high lipid content; JcW, wild type and JcVL, variant with low lipid content) with different lipid content, comparative proteomics was employed to profile the changes of oil body (OB) associated protein species by using gels-based proteomic technique. Eighty-three protein species were successfully identified through LTQ-ES-MS/MS from mature JcW seeds purified OBs. Two-dimensional electrophoresis analysis of J. curcas OB associated protein species revealed they had essential interactions with other organelles and demonstrated that oleosin and caleosin were the most abundant OB structural protein species. Twenty-eight OB associated protein species showed significant difference among JcVH, JcW and JcVL according to statistical analysis. Complementary transient expression analysis revealed that calcium ion binding protein (CalBP) and glycine-rich RNA binding protein (GRP) were well targeted in OBs apart from the oleosins. This study demonstrated that ratio of lipid content to caleosins abundance was involved in the regulation of OB size, and the mutant induced by ethylmethylsulfone treatment might be related to the caleosin like protein species. These findings are important for biotechnological improvement with the aim to alter the lipid content in J. curcas seeds. The economic value of Jatropha curcas largely depends on the lipid content in seeds which are mainly stored in the special organelle called oil bodies (OBs). In consideration of the biological importance and applications of J. curcas OB in seeds, it is necessary to further explore the components and functions of J. curcas OBs. Although a previous study concerning the J. curcas OB proteome revealed oleosins were the major OB protein component and additional protein species were similar to those in other oil seed plants, these identified OB associated protein species were corresponding to the protein bands instead of protein spots in the electrophoresis gels. Furthermore, the interaction of OB associated protein species and their contribution to OB formation and stabilization are still blank. In this study, with the overall object of profiling OB protein species from mature J. curcas seeds with different lipid content, we provided a setting of comparative OB proteomics with biochemical data and transient expression to explore the core of OB associated protein species involved in the regulation of OB size and lipid accumulation. The results were important for biotechnological improvement with the aim to a global modification of lipid storage in J. curcas seeds. Meanwhile, this study gave insight into possible associations between OBs and other organelles in mature J. curcas seeds. It may represent new aspects of the biological functions of the OBs during the oil mobilization. Combined the technique of transient transformation, a newly reported protein species, glycine-rich RNA binding protein (GRP) was successfully targeted in OBs. Therefore, further molecular analysis of these protein species is warranted to verify this association and what role they have in OBs. Copyright © 2014 Elsevier B.V. All rights reserved.
Advances in detection of diffuse seafloor venting using structured light imaging.
NASA Astrophysics Data System (ADS)
Smart, C.; Roman, C.; Carey, S.
2016-12-01
Systematic, remote detection and high resolution mapping of low temperature diffuse hydrothermal venting is inefficient and not currently tractable using traditional remotely operated vehicle (ROV) mounted sensors. Preliminary results for hydrothermal vent detection using a structured light laser sensor were presented in 2011 and published in 2013 (Smart) with continual advancements occurring in the interim. As the structured light laser passes over active venting, the projected laser line effectively blurs due to the associated turbulence and density anomalies in the vent fluid. The degree laser disturbance is captured by a camera collecting images of the laser line at 20 Hz. Advancements in the detection of the laser and fluid interaction have included extensive normalization of the collected laser data and the implementation of a support vector machine algorithm to develop a classification routine. The image data collected over a hydrothermal vent field is then labeled as seafloor, bacteria or a location of venting. The results can then be correlated with stereo images, bathymetry and backscatter data. This sensor is a component of an ROV mounted imaging suite which also includes stereo cameras and a multibeam sonar system. Originally developed for bathymetric mapping, the structured light laser sensor, and other imaging suite components, are capable of creating visual and bathymetric maps with centimeter level resolution. Surveys are completed in a standard mowing the lawn pattern completing a 30m x 30m survey with centimeter level resolution in under an hour. Resulting co-registered data includes, multibeam and structured light laser bathymetry and backscatter, stereo images and vent detection. This system allows for efficient exploration of areas with diffuse and small point source hydrothermal venting increasing the effectiveness of scientific sampling and observation. Recent vent detection results collected during the 2013-2015 E/V Nautilus seasons will be presented. Smart, C. J. and Roman, C. and Carey, S. N. (2013) Detection of diffuse seafloor venting using structured light imaging, Geochemistry, Geophysics, Geosystems, 14, 4743-4757
Pan, Jianjun
2018-01-01
This paper focuses on evaluating the ability and contribution of using backscatter intensity, texture, coherence, and color features extracted from Sentinel-1A data for urban land cover classification and comparing different multi-sensor land cover mapping methods to improve classification accuracy. Both Landsat-8 OLI and Hyperion images were also acquired, in combination with Sentinel-1A data, to explore the potential of different multi-sensor urban land cover mapping methods to improve classification accuracy. The classification was performed using a random forest (RF) method. The results showed that the optimal window size of the combination of all texture features was 9 × 9, and the optimal window size was different for each individual texture feature. For the four different feature types, the texture features contributed the most to the classification, followed by the coherence and backscatter intensity features; and the color features had the least impact on the urban land cover classification. Satisfactory classification results can be obtained using only the combination of texture and coherence features, with an overall accuracy up to 91.55% and a kappa coefficient up to 0.8935, respectively. Among all combinations of Sentinel-1A-derived features, the combination of the four features had the best classification result. Multi-sensor urban land cover mapping obtained higher classification accuracy. The combination of Sentinel-1A and Hyperion data achieved higher classification accuracy compared to the combination of Sentinel-1A and Landsat-8 OLI images, with an overall accuracy of up to 99.12% and a kappa coefficient up to 0.9889. When Sentinel-1A data was added to Hyperion images, the overall accuracy and kappa coefficient were increased by 4.01% and 0.0519, respectively. PMID:29382073
Odor-Induced Neuronal Rhythms in the Olfactory Bulb Are Profoundly Modified in ob/ob Obese Mice
Chelminski, Yan; Magnan, Christophe; Luquet, Serge H.; Everard, Amandine; Meunier, Nicolas; Gurden, Hirac; Martin, Claire
2017-01-01
Leptin, the product of the Ob(Lep) gene, is a peptide hormone that plays a major role in maintaining the balance between food intake and energy expenditure. In the brain, leptin receptors are expressed by hypothalamic cells but also in the olfactory bulb, the first central structure coding for odors, suggesting a precise function of this hormone in odor-evoked activities. Although olfaction plays a key role in feeding behavior, the ability of the olfactory bulb to integrate the energy-related signal leptin is still missing. Therefore, we studied the fate of odor-induced activity in the olfactory bulb in the genetic context of leptin deficiency using the obese ob/ob mice. By means of an odor discrimination task with concomitant local field potential recordings, we showed that ob/ob mice perform better than wild-type (WT) mice in the early stage of the task. This behavioral gain of function was associated in parallel with profound changes in neuronal oscillations in the olfactory bulb. The distribution of the peaks in the gamma frequency range was shifted toward higher frequencies in ob/ob mice compared to WT mice before learning. More notably, beta oscillatory activity, which has been shown previously to be correlated with olfactory discrimination learning, was longer and stronger in expert ob/ob mice after learning. Since oscillations in the olfactory bulb emerge from mitral to granule cell interactions, our results suggest that cellular dynamics in the olfactory bulb are deeply modified in ob/ob mice in the context of olfactory learning. PMID:28154537
Reduction of obesity, as induced by leptin, reverses endothelial dysfunction in obese (Lep(ob)) mice
NASA Technical Reports Server (NTRS)
Winters, B.; Mo, Z.; Brooks-Asplund, E.; Kim, S.; Shoukas, A.; Li, D.; Nyhan, D.; Berkowitz, D. E.
2000-01-01
Obesity is a major health care problem and is associated with significant cardiovascular morbidity. Leptin, a neuroendocrine hormone released by adipose tissue, is important in modulating obesity by signaling satiety and increasing metabolism. Moreover, leptin receptors are expressed on vascular endothelial cells (ECs) and mediate angiogenesis. We hypothesized that leptin may also play an important role in vasoregulation. We investigated vasoregulatory mechanisms in the leptin-deficient obese (ob/ob) mouse model and determined the influence of leptin replacement on endothelial-dependent vasorelaxant responses. The direct effect of leptin on EC nitric oxide (NO) production was also tested by using 4, 5-diaminofluorescein-2 diacetate staining and measurement of nitrate and nitrite concentrations. Vasoconstrictor responses to phenylephrine, norepinephrine, and U-46619 were markedly enhanced in aortic rings from ob/ob mice and were modulated by NO synthase inhibition. Vasorelaxant responses to ACh were markedly attenuated in mesenteric microvessels from ob/ob mice. Leptin replacement resulted in significant weight loss and reversal of the impaired endothelial-dependent vasorelaxant responses observed in ob/ob mice. Preincubation of ECs with leptin enhanced the release of NO production. Thus leptin-deficient ob/ob mice demonstrate marked abnormalities in vasoregulation, including impaired endothelial-dependent vasodilation, which is reversed by leptin replacement. These findings may be partially explained by the direct effect of leptin on endothelial NO production. These vascular abnormalities are similar to those observed in obese, diabetic, leptin-resistant humans. The ob/ob mouse may, therefore, be an excellent new model for the study of the cardiovascular effects of obesity.
NASA Astrophysics Data System (ADS)
Epps, S. A.
2017-12-01
Suspended particulate matter (SPM) is an important agent in generating marine light conditions which in turn have strong influences on biogeochemical systems. SPM also behaves as a vehicle for contaminant migration and is of interest to the estimation of bulk material transport in the marine environment. The measurement of inherent optical properties (IOPs) and apparent optical properties (AOPs) is becoming increasingly important in the prediction of SPM concentration. To more fully utilize data generated in bathymetric lidar surveys, modern systems such as CZMIL (the Coastal Zone Mapping Imaging LIDAR) include a hyperspectral sensor to collect data necessary for remote sensing reflectance (Rrs), an AOP. Some IOPs can be estimated can be estimated from Rrs. Additionally, a bathymetric lidar return signal contains both absorption and backscattering components (IOPs) at 532 nm which may be utilized for SPM prediction. This research utilizes IOP measurements using AC-9, AC-S, BB-9, and LISST-100X-B sensors deployed in the Northern Gulf of Mexico concurrent with SPM collection via filtration. Concomitant Rrs values were collected using a hand held hyperspectral sensor. Several hundred linearly regressed single-parameter estimates are created to predict SPM concentration using the IOPs attenuation, total scatter, backscatter, absorption and significant amalgamations thereof. Multiple wavelengths of light are analyzed for each IOP or IOP combination. Consideration is given to the suitability of each IOP type to SPM concentration prediction. Several criteria are assessed to winnow out the best predictors. These include sensor, data, and environmental limitations. The quantitative analyses of this research assist to identify the best types of IOPs (and wavelengths) for SPM prediction. Rrs at multiple wavelengths is also considered for SPM prediction. This research is focused on the functionality of IOP and AOP based SPM concentration predictions made available from the data products of bathymetric lidar surveys. It has applications for researchers with interest in IOPs, AOPs and SPM. There are also implications for monitoring estuarine, coastal, and offshore environments using bathymetric lidar and in-situ optical sensor suites to estimate SPM.
iNOS inhibits hair regeneration in obese diabetic (ob/ob) mice.
Sasaki, Mari; Shinozaki, Shohei; Morinaga, Hironobu; Kaneki, Masao; Nishimura, Emi; Shimokado, Kentaro
2018-07-02
Previous studies have shown that androgenic alopecia is associated with metabolic syndrome and diabetes. However, the detailed mechanism whereby diabetes causes alopecia still remains unclear. We focused on the inflammatory response that is caused by diabetes or obesity, given that inflammation is a risk factor for hair loss. Inducible nitric oxide synthase (iNOS) is known to be upregulated under conditions of acute or chronic inflammation. To clarify the potential role of iNOS in diabetes-related alopecia, we generated obese diabetic iNOS-deficient (ob/ob; iNOS-KO mice). We observed that ob/ob; iNOS-KO mice were potentiated for the transition from telogen (rest phase) to anagen (growth phase) in the hair cycle compared with iNOS-proficient ob/ob mice. To determine the effect of nitric oxide (NO) on the hair cycle, we administered an iNOS inhibitor intraperitoneally (compound 1400 W, 10 mg/kg) or topically (10% aminoguanidine) in ob/ob mice. We observed that iNOS inhibitors promoted anagen transition in ob/ob mice. Next, we administered an NO donor (S-nitrosoglutathione, GSNO), to test whether NO has the telogen elongation effects. The NO donor was sufficient to induce telogen elongation in wild-type mice. Together, our data indicate that iNOS-derived NO plays a role in telogen elongation under the inflammatory conditions associated with diabetes in mice. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.
Church, Stephanie J; Begley, Paul; Kureishy, Nina; McHarg, Selina; Bishop, Paul N; Bechtold, David A; Unwin, Richard D; Cooper, Garth J S
2015-05-08
Ob/ob mice provide an animal model for non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH) in patients with obesity and type-2 diabetes. Low liver copper has been linked to hepatic lipid build-up (steatosis) in animals with systemic copper deficiency caused by low-copper diets. However, hepatic copper status in patients with NAFLD or NASH is uncertain, and a validated animal model useful for the study of hepatic copper regulation in common forms of metabolic liver disease is lacking. Here, we report parallel measurements of essential metal levels in whole-liver tissue and defatted-dried liver tissue from ob/ob and non-obese control mice. Measurements in whole-liver tissue from ob/ob mice at an age when they have developed NAFLD/NASH, provide compelling evidence for factitious lowering of copper and all other essential metals by steatosis, and so cannot be used to study hepatic metal regulation in this model. By marked contrast, metal measurements in defatted-dried liver samples reveal that most essential metals were actually normal and indicate specific lowering of copper in ob/ob mice, consistent with hepatic copper deficiency. Thus ob/ob mice can provide a model useful for the study of copper regulation in NAFLD and NASH, provided levels are measured in defatted-dried liver tissue. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
Gratuze, Maud; El Khoury, Noura B; Turgeon, Andréanne; Julien, Carl; Marcouiller, François; Morin, Françoise; Whittington, Robert A; Marette, André; Calon, Frédéric; Planel, Emmanuel
2017-02-01
Over the last few decades, there has been a significant increase in epidemiological studies suggesting that type 2 diabetes (T2DM) is linked to a higher risk of Alzheimer's disease (AD). However, how T2DM affects AD pathology, such as tau hyperphosphorylation, is not well understood. In this study, we investigated the impact of T2DM on tau phosphorylation in ob/ob mice, a spontaneous genetic model of T2DM. Tau phosphorylation at the AT8 epitope was slightly elevated in 4-week-old ob/ob mice while 26-week-old ob/ob mice exhibited tau hyperphosphorylation at multiple tau phospho-epitopes (Tau1, CP13, AT8, AT180, PHF1). We then examined the mechanism of tau hyperphosphorylation and demonstrated that it is mostly due to hypothermia, as ob/ob mice were hypothermic and normothermia restored tau phosphorylation to control levels. As caffeine has been shown to be beneficial for diabetes, obesity and tau phosphorylation, we, therefore, used it as therapeutic treatment. Unexpectedly, chronic caffeine intake exacerbated tau hyperphosphorylation by promoting deeper hypothermia. Our data indicate that tau hyperphosphorylation is predominately due to hypothermia consequent to impaired thermoregulation in ob/ob mice. This study establishes a novel link between diabetes and AD, and reinforces the importance of recording body temperature to better assess the relationship between diabetes and AD. Copyright © 2016 Elsevier Inc. All rights reserved.
Zhao, Huichen; Chen, Jicui; Chai, Jiachao; Zhang, Yuchao; Yu, Cong; Pan, Zhe; Gao, Peng; Zong, Chen; Guan, Qingbo; Fu, Yuqin; Liu, Yuantao
2017-07-01
Epoxyeicosatrienoic acids (EETs) are the epoxidation products of arachidonic acid catalyzed by cytochrome P450 (CYP) epoxygenases, which possess multiple biological activities. In the present study, we aimed to explore the role and effects of CYP epoxygenases/EETs in wound healing in ob/ob mice. Full-thickness skin dorsal wounds were made on ob/ob mice and C57BL/6 control mice. The mRNA and protein expression of CYP epoxygenases were determined in granulation tissues of wounds. Effects of EETs on wound healing were evaluated. Inflammation and angiogenesis in wounds were also observed. Compared with C57BL/6 mice, the mRNA and protein expression of CYP2C65 and CYP2J6 in the granulation tissues in ob/ob mice were significantly reduced. 11,12-EET treatment significantly improved wound healing in ob/ob mice, whereas 14,15-EEZE, an EET antagonist, showed the opposite effect. 11,12-EET treatment decreased neutrophil and macrophage infiltration to the wound sites, resulting in reduced production of inflammatory cytokines, decreased MMP-9 expression, and increased collagen accumulation in the granulation tissues of ob/ob mice. In addition, 11,12-EET increased angiogenesis in the granulation tissues of wounds in ob/ob mice. These findings indicate that reduced expression of CYP epoxygenases may contribute to impaired diabetic wound healing, and exogenous EETs may improve diabetic wound healing by modulating inflammation and angiogenesis.
Sentinel-1 backscatter sensitivity to vegetation dynamics at the field scale.
NASA Astrophysics Data System (ADS)
Vreugdenhil, Mariette; Eder, Alexander; Bauer-Marschallinger, Bernhard; Cao, Senmao; Naeimi, Vahid; Oismueller, Markus; Strauss, Peter; Wagner, Wolfgang
2017-04-01
Vegetation monitoring is pivotal to improve our understanding of the role vegetation dynamics play in the global carbon-, energy- and hydrological cycle. And with the increasing stress on food supply due to the growing world populating and changing climate, vegetation monitoring is of great importance in agricultural areas. By closely tracking crop conditions, droughts and subsequent crop losses could be mitigated. Sensors operating in the microwave domain are sensitive to several surface characteristics, including soil moisture and vegetation. Hence, spaceborne microwave remote sensing provides the means to monitor vegetation and soil conditions on different scales, ranging from field scale to global scale. However, it also presents a challenge since multiple combinations of soil and vegetation characteristics can lead to a similar measurement. Copernicus Sentinel-1 (S-1) is a series of two satellites, developed by the European Space Agency (ESA) , which carry C-band Synthetic Aperture Radars. The C-SAR sensors provide VV, HH, VH and HV backscatter at a 5 m by 20 m spatial resolution. The temporal revisit time of the two satellites is 3-6 days. With their unique capacity for temporally dense and spatially detailed data, the S-1 satellite series provides for the first time the chance to investigate vegetation dynamics at high temporal and spatial resolution. The aim of this study is to assess the sensitivity of Sentinel-1 backscatter to vegetation dynamics. The study is performed in the Hydrological Open Air Laboratory (HOAL), which is a 66 hectare large catchment located in Petzenkirchen, Austria. In the HOAL several vegetation parameters were measured during the course of the growing season (2016) at the overpass time of S-1a. Vegetation height was obtained ten times for the whole catchment, using georeferenced photos made by a motorized paraglider and a Land Surface Model. In addition, vegetation water content, Leaf Area Index and soil moisture were measured in four different cropfields. An in situ soil moisture network provides continuous soil moisture measurements at 31 locations within the catchment. Different polarizations and ratios thereof were calculated and compared, both spatially and temporally, to the in situ measurements of vegetation height, LAI, vegetation water content and soil moisture. Preliminary results show a clear spatial pattern in cross-polarized backscatter, which is related to different crop types. Time series analysis suggests that a ratio between cross- and co-polarized backscatter is affected by both vegetation water content and vegetation structure. This presentation will provide a comprehensive assessment of Sentinel-1's capability for monitoring of vegetation over croplands, using in situ reference data obtained over a full growing season.
NASA Technical Reports Server (NTRS)
Palm, Stephen P.; Schwemmer, Geary K.; Vandemark, Doug; Evans, Keith; Miller, David O.; Demoz, Belay B.; Starr, David OC. (Technical Monitor)
2001-01-01
A new technique combining active and passive remote sensing instruments for the estimation of surface latent heat flux over the ocean is presented. This synergistic method utilizes aerosol lidar backscatter data, multi-channel infrared radiometer data, and microwave scatterometer data acquired onboard the NASA P-313 research aircraft during an extended field campaign over the Atlantic ocean in support of the Lidar In-space Technology Experiment (LITE) in September of 1994. The 10 meter wind speed derived from scatterometers and lidar-radiometer inferred near-surface moisture are used to obtain an estimate of the surface flux of moisture via a bulk aerodynamic formula. The results are compared with the Special Sensor Microwave Imager (SSM/I) daily average latent heat flux and show reasonable agreement. However, the SSM/I values are biased low by about 15 W/sq m. In addition, the Marine Atmospheric Boundary Layer (MABL) height, entrainment zone thickness and integrated lidar backscatter intensity are computed from the lidar data and compared with the magnitude of the surface fluxes. The results show that the surface latent heat flux is most strongly correlated with entrainment zone depth, MABL height and the integrated MABL lidar backscatter, with corresponding correlation coefficients of 0.39, 0.43 and 0.71, respectively.
NASA Technical Reports Server (NTRS)
Palm, Stephen P.; Miller, David O.; Schwemmer, Geary
2000-01-01
A new technique combining active and passive remote sensing instruments for the estimation of surface latent heat flux over the ocean is presented. This synergistic method uses aerosol lidar backscatter data, multi-channel infrared radiometer data and microwave scatterometer data acquired onboard the NASA P-3B research aircraft during an extended field campaign over the Atlantic ocean in support of the Lidar In-space Technology Experiment (LITE) in September of 1994. The 10 meter wind speed derived from the scatterometers and the lidar-radiometer inferred near-surface moisture are used to obtain an estimate of the surface flux of moisture via bulk aerodynamic formulae. The results are compared with the Special Sensor Microwave Imager (SSM/I) daily average latent heat flux and show reasonable agreement with an rms error and bias of about 50 and 25 W per square meters, respectively. In addition, the MABL height, entrainment zone thickness and integrated lidar backscatter intensity are computed from the lidar data and compared with the magnitude of the surface fluxes. The results show that the surface latent heat flux is most strongly correlated with entrainment zone top, bottom and the integrated MABL lidar backscatter, with corresponding correlation coefficients of 0.62, 0.67 and 0.61, respectively.
Aerosol Backscatter Profiles at 10.59 and 9.25 Micrometers near Mauna Loa, Hawaii, 1988
NASA Astrophysics Data System (ADS)
Post, Madison J.
1989-12-01
The NOAA Doppler lidar trailer was transported from Boulder, Colorado, to the 3.231km level of Hawaii's Mauna Loa volcano (lat. 19.55°N, long. 155.56°W) in No-vember 1988 to participate in the NASA-sponsored Mauna Loa Backscatter Intercomparison Experiment (MABIE) for 1988. Our purpose was multifold. Among the aerosol studies our goals were to gather a statistically meaningful set of vertical backscatter pro-files at two wavelengths in the clean Pacific environment, to compare data from several microphysical sensors located at the GMCC observatory 3 km away, to assess the representativeness of the ground-based GMCC samplers with respect to the air mass over-head, and to understand the depth of the upslope and downslope flows that have historically affected the GMCC samplers. We were highly successful on all counts, having gathered 243 vertical profiles at 10.59 gm, 49 profiles at 9.25 vim, 278 GMCC intercom-parisons, and 404 wind profiles and cross sections. Our data-gathering period extended over 24 days through December 11. We calibrated the system on seven different days, usually at both wavelengths, to insure accuracy in our results. We also acquired data close in time to nearby SAGE 11. sampling, and twice took data simultaneously with GMCC's ruby lidar.
Sestili, Piero; Ismail, Tariq; Calcabrini, Cinzia; Guescini, Michele; Catanzaro, Elena; Turrini, Eleonora; Layla, Anam; Akhtar, Saeed; Fimognari, Carmela
2018-07-01
Basil (Ocimum basilicum L., OB) is a plant world widely used as a spice and a typical ingredient of the healthy Mediterranean diet. In traditional medicine, OB is indicated for many maladies and conditions; OB-containing nutritional supplements are increasingly sold. Conversely, safety concerns have been raised about the promutagens and procarcinogens alkenylbenzenes contained in OB. Areas covered: A critical review of the current status of OB as a nutraceutical, the pharmacology of its bioactive components, the rationale for its indications, and its safety. Expert opinion: Due to the polyphenolic and flavonoidic content, OB can be considered as an important ingredient in healthy diets; OB preparations may be effective as chemopreventive agents or adjunctive therapy in the treatment of different clinical conditions. From a toxicological perspective, since the tumorigenic potential of alkenylbenzenes is counteracted by other OB constituents such as nevadensin, it can be concluded that OB consumption in food and preparations is safe. The only concern relates to OB essential oils: in this case, a concentration limit for alkenylbenzenes should be precautionary defined, and the use of plant chemotypes with no or low levels of these alkylbenzenes for the preparation of essential oils should be made compulsory.
Leptin Increases Striatal Dopamine D2 Receptor Binding in Leptin-Deficient Obese (ob/ob) Mice
DOE Office of Scientific and Technical Information (OSTI.GOV)
Pfaffly, J.; Michaelides, M.; Wang, G-J.
2010-06-01
Peripheral and central leptin administration have been shown to mediate central dopamine (DA) signaling. Leptin-receptor deficient rodents show decreased DA D2 receptor (D2R) binding in striatum and unique DA profiles compared to controls. Leptin-deficient mice show increased DA activity in reward-related brain regions. The objective of this study was to examine whether basal D2R-binding differences contribute to the phenotypic behaviors of leptin-deficient ob/ob mice, and whether D2R binding is altered in response to peripheral leptin treatment in these mice. Leptin decreased body weight, food intake, and plasma insulin concentration in ob/ob mice but not in wild-type mice. Basal striatal D2Rmore » binding (measured with autoradiography [{sup 3}H] spiperone) did not differ between ob/ob and wild-type mice but the response to leptin did. In wild-type mice, leptin decreased striatal D2R binding, whereas, in ob/ob mice, leptin increased D2R binding. Our findings provide further evidence that leptin modulates D2R expression in striatum and that these effects are genotype/phenotype dependent.« less
The Cygnus OB2 Star Forming Complex
NASA Astrophysics Data System (ADS)
Rybarczyk, Daniel R.; Bania, Thomas
2018-01-01
Almost all astrophysical systems—from planets to stars to supernovae to entire galaxies—are impacted by the process of star formation. The brightest, most massive stars (OB stars) form in hot young clusters called OB associations. Cygnus OB2 is an OB association containing over 160 OB stars, making it one of the largest in the Milky Way Galaxy. At a distance of less than 1.5 kpc, its proximity to the Sun makes it optimal for assessing the process of Galactic star formation and its implications for stellar evolution, Galactic structure, and Galactic chemical evolution. Using existing data sets, we derive comprehensive maps of the distribution of thermal continuum, atomic, and molecular emission from the interstellar gas in Cyg OB2. The thermal continuum emission stems from the plasma ionized by OB stars. The atomic gas is probed by emission from atomic hydrogen, HI, at 21 cm wavelength. The molecular gas is traced by emission from the CO molecule which is a proxy for molecular hydrogen, H2. We combine these atomic and molecular data to derive a map of the total proton column density distribution in Cyg OB2. We also analyze the velocity fields of the OB stars, the atomic and molecular hydrogen gas, and the HII regions' radio recombination emission. As expected, we find HII regions to be spatially coincident with zones of higher cloud density. Surrounding the greatest concentration of OB stars is a cavity in the radio continuum and CO emission. This results from shock waves produced by the combined action of the high HII region pressure and winds from the OB stars. Such a distribution implies that Cyg OB2 is old enough to have evolved to this state.
NASA Astrophysics Data System (ADS)
Faustov, A.; Gussarov, A.; Wuilpart, M.; Fotiadi, A. A.; Liokumovich, L. B.; Kotov, O. I.; Zolotovskiy, I. O.; Tomashuk, A. L.; Deschoutheete, T.; Mégret, P.
2012-04-01
On-line monitoring of environmental conditions in nuclear facilities is becoming a more and more important problem. Standard electronic sensors are not the ideal solution due to radiation sensitivity and difficulties in installation of multiple sensors. In contrast, radiation-hard optical fibres can sustain very high radiation doses and also naturally offer multi-point or distributed monitoring of external perturbations. Multiple local electro-mechanical sensors can be replaced by just one measuring fibre. At present, there are over four hundred operational nuclear power plants (NPPs) in the world 1. Operating experience has shown that ineffective control of the ageing degradation of major NPP components can threaten plant safety and also plant life. Among those elements, cables are vital components of I&C systems in NPPs. To ensure their safe operation and predict remaining life, environmental monitoring is necessary. In particular, temperature and radiation dose are considered to be the two most important parameters. The aim of this paper is to assess experimentally the feasibility of optical fibre temperature measurements in a low doserate radiation environment, using a commercially available reflectometer based on Rayleigh backscattering. Four different fibres were installed in the Sub-Pile Room of the BR2 Material testing nuclear reactor in Mol, Belgium. This place is man-accessible during the reactor shut-down, allowing easy fibre installation. When the reactor operates, the dose-rates in the room are in a range 0.005-5 Gy/h with temperatures of 40-60 °C, depending on the location. Such a surrounding is not much different to some "hot" environments in NPPs, where I&C cables are located.
Multiscale radar mapping of surface melt over mountain glaciers in High Mountain Asia
NASA Astrophysics Data System (ADS)
Steiner, N.; McDonald, K. C.
2017-12-01
Glacier melt dominates input for many hydrologic systems in the Himalayan Hindukush region that feed rivers that are critical for downstream ecosystems and hydropower generation in this highly populated area. Deviation in seasonal surface melt timing and duration with a changing climate has the potential to affect up to a billion people on the Indian Subcontinent. Satellite-borne microwave remote sensing has unique capabilities that allow monitoring of numerous landscape processes associated with snowmelt and freeze/thaw state, without many of the limitations in optical-infrared sensors such as solar illumination or atmospheric conditions. The onset of regional freeze/thaw and surface melting transitions determine important surface hydrologic variables like river discharge. Theses regional events are abrupt therefore difficult to observe with low-frequency observation sensors. Recently launched synthetic aperture radar (SAR) onboard the Sentinel-1 A and B satellites from the European Space Agency (ESA) provide wide-swath and high spatial resolution (50-100 m) C-Band SAR observations with observations frequencies not previously available, on the order of 8 to 16 days. The Sentinel SARs provide unique opportunity to study freeze/thaw and mountain glacier melt dynamics at process level scales, spatial and temporal. The melt process of individual glaciers, being fully resolved by imaging radar, will inform on the radiometric scattering physics associated with surface hydrology during the transition from melted to thawed state and during refreeze. Backscatter observations, along with structural information about the surface will be compared with complimentary coarse spatial resolution C-Band radar scatterometers, Advanced Scatterometer (ASCAT Met Op A+B), to understand the sub-pixel contribution of surface melting and freeze/thaw signals. This information will inform on longer-scale records of backscatter from ASCAT, 2006-2017. We present a comparison of polarimetric C-Band melt signals contained in the multi-scale backscatter and present a coincident freeze/thaw and snowmelt records from ASCAT and Sentinel-1 for the Gandaki basin, Nepal.
Curtis, Jennifer A.
2007-01-01
The U.S. Geological Survey, in cooperation with Point Reyes National Seashore, is studying suspended-sediment transport dynamics in the two primary tributaries to Tomales Bay, Lagunitas Creek and Walker Creek. Suspended-sediment samples and continuous optical backscatter (turbidity) data were collected at three locations during water years 2004?06 (October 1, 2003?September 30, 2006): at two sites in the Lagunitas Creek watershed and at one site in the Walker Creek watershed. Sediment samples were analyzed for suspended-sediment concentration, grain size, and turbidity. Data were used to estimate mean daily and annual seasonal suspended-sediment discharge, which were published in U.S. Geological Survey Annual Water-Data Reports. Data were utilized further in this report to develop field-based optical-backscatter calibration equations, which then were used to derive a continuous time series (15-minute interval) of suspended-sediment concentrations. Sensor fouling and aggradation of the channel bed occurred periodically throughout the project period, resulting in data loss. Although periods of data loss occurred, collection of optical sensor data improved our understanding of suspended-sediment dynamics in the Lagunitas Creek and Walker Creek watersheds by providing continuous time-series storm event data that were analyzed to determine durations of elevated sediment concentrations (periods of time when suspended-sediment concentration was greater than 100 mg/L). Data derived from this project contributed baseline suspended-sediment transport information that will be used to develop and implement sediment total maximum daily loads for Tomales Bay and its tributary watersheds, and provides supporting information for additional total maximum daily loads (pathogens, nutrients, and mercury) and restoration efforts for four federally listed aquatic species that are affected directly by sediment loading in the Tomales Bay watershed. In addition, this project provided an opportunity to evaluate the suitability of using optical data as a surrogate for more traditional labor-intensive methods of measuring suspended-sediment transport in steep coastal watersheds.
Development of an L-, C-, and X-band radar for backscattering studies over vegetation
NASA Technical Reports Server (NTRS)
Lockhart, G. Lance
1995-01-01
With the recent surge of interest in global change, the impact of different ecosystems on the Earth's carbon budget has become the focus of many scientific studies. Studies have been launched by NASA and other agencies to address this issue. One such study is the Boreal Ecosystem-Atmosphere Study (BOREAS). BOREAS focuses on the boreal ecosystem in Northern Canada. As a part of the BOREAS study, we have developed a helicopter-borne three-band radar system for measuring the scattering coefficient of various stands within the boreal forest. During the summer of 1994 the radar was used at the southern study area (SSA) in Saskatchewan over the young jack pine (YJP), old jack pine (OJP), old black spruce (OBS) and old aspen (OA) sites. The data collected will be used to study the interaction of microwaves with forest canopy. By making use of three different frequency bands the contribution to the backscatter from each of the layers within the canopy can be determined. Using the knowledge gained from these studies, we will develop algorithms to enable more accurate interpretation of SAR images of the boreal region. This report describes in detail the development of the L-, C- and X-band radar system. The first section provides background information and explains the objectives of the boreal forest experiment. The second section describes the design and implementation of the radar system. All of the subsystems of the radar are explained in this section. Next, problems that were encountered during system testing and the summer experiments are discussed. System performance and results are then presented followed by a section on conclusions and further work.
Tagaloa, Sherry; Zhang, Linda; Dare, Anna J.; MacDonald, Julia R.; Yeong, Mee-Ling; Bartlett, Adam S. J. R.; Phillips, Anthony R. J.
2014-01-01
Background Hepatic steatosis is a major risk factor for graft failure in liver transplantation. Hepatic steatosis shows a greater negative influence on graft function following prolonged cold ischaemia. As the impact of steatosis on hepatocyte metabolism during extended cold ischaemia is not well-described, we compared markers of metabolic capacity and mitochondrial function in steatotic and lean livers following clinically relevant durations of cold preservation. Methods Livers from 10-week old leptin-deficient obese (ob/ob, n = 9) and lean C57 mice (n = 9) were preserved in ice-cold University of Wisconsin solution. Liver mitochondrial function was then assessed using high resolution respirometry after 1.5, 3, 5, 8, 12, 16 and 24 hours of storage. Metabolic marker enzymes for anaerobiosis and mitochondrial mass were also measured in conjunction with non-bicarbonate tissue pH buffering capacity. Results Ob/ob and lean mice livers showed severe (>60%) macrovesicular and mild (<30%) microvesicular steatosis on Oil Red O staining, respectively. Ob/ob livers had lower baseline enzymatic complex I activity but similar adenosine triphosphate (ATP) levels compared to lean livers. During cold storage, the respiratory control ratio and complex I-fueled phosphorylation deteriorated approximately twice as fast in ob/ob livers compared to lean livers. Ob/ob livers also demonstrated decreased ATP production capacities at all time-points analyzed compared to lean livers. Ob/ob liver baseline lactate dehydrogenase activities and intrinsic non-bicarbonate buffering capacities were depressed by 60% and 40%, respectively compared to lean livers. Conclusions Steatotic livers have impaired baseline aerobic and anaerobic capacities compared to lean livers, and mitochondrial function indices decrease particularly from after 5 hours of cold preservation. These data provide a mechanistic basis for the clinical recommendation of shorter cold storage durations in steatotic donor livers. PMID:24956382
Deng, Xiuling; Wang, Jiliang; Jiao, Li; Utaipan, Tanyarath; Tuma-Kellner, Sabine; Schmitz, Gerd; Liebisch, Gerhard; Stremmel, Wolfgang; Chamulitrat, Walee
2016-05-01
PLA2G6 or GVIA calcium-independent PLA2 (iPLA2β) is identified as one of the NAFLD modifier genes in humans, and thought to be a target for NAFLD therapy. iPLA2β is known to play a house-keeping role in phospholipid metabolism and remodeling. However, its role in NAFLD pathogenesis has not been supported by results obtained from high-fat feeding of iPLA2β-null (PKO) mice. Unlike livers of human NAFLD and genetically obese rodents, fatty liver induced by high-fat diet is not associated with depletion of hepatic phospholipids. We therefore tested whether iPLA2β could regulate obesity and hepatic steatosis in leptin-deficient mice by cross-breeding PKO with ob/ob mice to generate ob/ob-PKO mice. Here we observed an improvement in ob/ob-PKO mice with significant reduction in serum enzymes, lipids, glucose, insulin as well as improved glucose tolerance, and reduction in islet hyperplasia. The improvement in hepatic steatosis measured by liver triglycerides, fatty acids and cholesterol esters was associated with decreased expression of PPARγ and de novo lipogenesis genes, and the reversal of β-oxidation gene expression. Notably, ob/ob livers contained depleted levels of lysophospholipids and phospholipids, and iPLA2β deficiency in ob/ob-PKO livers lowers the former, but replenished the latter particularly phosphatidylethanolamine (PE) and phosphatidylcholine (PC) that contained arachidonic (AA) and docosahexaenoic (DHA) acids. Compared with WT livers, PKO livers also contained increased PE and PC containing AA and DHA. Thus, iPLA2β deficiency protected against obesity and ob/ob fatty liver which was associated with hepatic fatty-acyl phospholipid remodeling. Our results support the deleterious role of iPLA2β in severe obesity associated NAFLD. Copyright © 2016 Elsevier B.V. All rights reserved.
Clinical effect of four different ointment bases on healthy cat eyes.
Eördögh, Réka; Schwendenwein, Ilse; Tichy, Alexander; Loncaric, Igor; Nell, Barbara
2016-07-01
To describe the effects of long-term treatment with four different eye ointment bases (OBs) in cats. Ten healthy cats. The study was performed in two periods. Four different OBs were tested. Hundred grams of OB contained the following: OB-A: 35.17 g liquid paraffin (lp), 64.83 g white petrolatum (wp); OB-B: 10.03 g lp, 84.95 g wp 5.02 g lanolin; OB-C: 18.34 g lp, 51.40 g wp, 25.00 mg KH2 PO4 , 57.00 mg K2 HPO4 , 18.90 g eucerinum anhydricum, 11.28 g water for injections; and OB-D: 70 g unguentum lanalcoli, 20 g lp, 10 g aqua conservans. One eye was treated, and the other served as a negative control. Cats received the OBs TID for 28 days. The two study periods were separated by a 4-month washout phase. Samples for conjunctival impression cytology, swabs for bacteriologic and mycologic examination, and cytobrush samples for FHV-1 and Chlamydophila felis PCR detection were obtained. Both eyes were examined daily. Severity of ocular symptoms was scored using a modified Draize eye irritation test. A total of five eyes were treated with OB-A, five with OB-B, four with OB-C, and five with OB-D. Treated eyes had significantly higher clinical scores. Eyes receiving OB-A had the highest overall clinical score. The results of bacteriologic and mycologic examination concur with the previously published data. All samples tested were negative for FHV-1 and Chlamydophila felis. There was no significant difference between treated and control eyes upon cytological examination. The application of OBs resulted in clinical symptoms in treated eyes. The long-term use of ointments is not well tolerated in cats and may lead to ocular irritation. © 2015 American College of Veterinary Ophthalmologists.
Kharlamova, A S; Barabanov, V M; Saveliev, S V
2015-01-01
We provide the data of the olfactory bulbs (OB) development in the human fetuses on the stages from 8 week to birth. Immunochistochemical markers of presynaptic terminals (anti-SNAP-25, -synapsin-I, -synaptophysin) were used to evaluate the maturation of the OB. Differentiation of the OB layers begins from periphery, which implicitly evidences that growth of the olfactory nerves fibers induses not only anatomical differentiation of the OB, but also differentiation of its functional layers. The sites of the developing glomerulus are revealed using the immunochistochemical prosedure on the stage before distinct glomerulus can be identified with common histological procedure. OB conductive system demonstrates immunoreactivity with the antibodies to the presynaptic proteins on the all stages from 10-11 weeks of fetus development. Four stages of the OB development are described. All functional layers of the OB are mature at the 22-weeks stage. Further differentiation of the OB neuroblasts, including lamina formation of the internal granular leyer, glomerular layer development, OB growth continue after 20-22 weeks stage until 38-40 weeks of the fetus develoment. Patterns of the immunoreactivity with antibodies to SNAP-25, synapsin-I and synaptophysin are completely appropriate to those of adult's OB on the 38-40 weeks of the prenatal development. Complete maturity of the human OB is achived at 38-40 weeks of the prenatal development.
Kinematics of OB-associations in Gaia epoch
NASA Astrophysics Data System (ADS)
Mel'nik, A. M.; Dambis, A. K.
2017-12-01
We use stellar proper motions from the Tycho-Gaia Astrometric Solution (TGAS) catalogue to study the kinematics of OB-associations. The TGAS proper motions of OB-associations generally agree well with the Hipparcos proper motions. The parameters of the Galactic rotation curve obtained with TGAS and Hipparcos proper motions agree within the errors. The average one-dimensional velocity dispersion inside 18 OB-associations with more than 10 TGAS stars is σv = 3.9 km s-1, which is considerably smaller, by a factor of 0.4, than the velocity dispersions derived from Hipparcos data. The effective contribution from orbital motions of binary OB-stars into the velocity dispersion σv inside OB-associations is σb = 1.2 km s-1. The median virial and stellar masses of OB-associations are equal to 7.1 × 105 and 9.0 × 103 M⊙, respectively. Thus, OB-associations must be unbound objects, provided they do not include a lot of dense gas. The median star-formation efficiency is ε = 2.1 per cent. Nearly one-third of stars of OB-associations must lie outside their tidal radius. We found that the Per OB1 and Car OB1 associations are expanding with the expansion started in a small region of 11-27 pc 7-10 Myr ago. The average expansion velocity is 6.3 km s-1.
Comparative analysis of multisensor satellite monitoring of Arctic sea-ice
Belchansky, G.I.; Mordvintsev, Ilia N.; Douglas, David C.
1999-01-01
This report represents comparative analysis of nearly coincident Russian OKEAN-01 polar orbiting satellite data, Special Sensor Microwave Imager (SSM/I) and Advanced Very High Resolution Radiometer (AVHRR) imagery. OKEAN-01 ice concentration algorithms utilize active and passive microwave measurements and a linear mixture model for measured values of the brightness temperature and the radar backscatter. SSM/I and AVHRR ice concentrations were computed with NASA Team algorithm and visible and thermal-infrared wavelength AVHRR data, accordingly
Hwang, Dusun; Yoon, Dong-Jin; Kwon, Il-Bum; Seo, Dae-Cheol; Chung, Youngjoo
2010-05-10
A novel method for auto-correction of fiber optic distributed temperature sensor using anti-Stokes Raman back-scattering and its reflected signal is presented. This method processes two parts of measured signal. One part is the normal back scattered anti-Stokes signal and the other part is the reflected signal which eliminate not only the effect of local losses due to the micro-bending or damages on fiber but also the differential attenuation. Because the beams of the same wavelength are used to cancel out the local variance in transmission medium there is no differential attenuation inherently. The auto correction concept was verified by the bending experiment on different bending points. (c) 2010 Optical Society of America.
Smart Garment Fabrics to Enable Non-Contact Opto-Physiological Monitoring.
Iakovlev, Dmitry; Hu, Sijung; Hassan, Harnani; Dwyer, Vincent; Ashayer-Soltani, Roya; Hunt, Chris; Shen, Jinsong
2018-03-29
Imaging photoplethysmography (iPPG) is an emerging technology used to assess microcirculation and cardiovascular signs by collecting backscattered light from illuminated tissue using optical imaging sensors. The aim of this study was to study how effective smart garment fabrics could be capturing physiological signs in a non-contact mode. The present work demonstrates a feasible approach of, instead of using conventional high-power illumination sources, integrating a grid of surface-mounted light emitting diodes (LEDs) into cotton fabric to spotlight the region of interest (ROI). The green and the red LEDs (525 and 660 nm) placed on a small cotton substrate were used to locally illuminate palm skin in a dual-wavelength iPPG setup, where the backscattered light is transmitted to a remote image sensor through the garment fabric. The results show that the illuminations from both wavelength LEDs can be used to extract heart rate (HR) reaching an accuracy of 90% compared to a contact PPG probe. Stretching the fabric over the skin surface alters the morphology of iPPG signals, demonstrating a significantly higher pulsatile amplitude in both channels of green and red illuminations. The skin compression by the fabric could be potentially utilised to enhance the penetration of illumination into cutaneous microvascular beds. The outcome could lead a new avenue of non-contact opto-physiological monitoring and assessment with functional garment fabrics.
New massive members of Cygnus OB2
NASA Astrophysics Data System (ADS)
Berlanas, S. R.; Herrero, A.; Comerón, F.; Pasquali, A.; Motta, C. Bertelli; Sota, A.
2018-04-01
Context. The Cygnus complex is one of the most powerful star forming regions at a close distance from the Sun ( 1.4 kpc). Its richest OB association Cygnus OB2 is known to harbor many tens of O-type stars and hundreds of B-type stars, providing a large homogeneous population of OB stars that can be analyzed. Many studies of its massive population have been developed in the last decades, although the total number of OB stars is still incomplete. Aim. Our aim is to increase the sample of O and B members of Cygnus OB2 and its surroundings by spectroscopically classifying 61 candidates as possible OB-type members of Cygnus OB2, using new intermediate resolution spectroscopy. Methods: We have obtained intermediate resolution (R 5000) spectra for all of the OB-type candidates between 2013 and 2017. We thus performed a spectral classification of the sample using HeI-II and metal lines rates, as well as the Marxist Ghost Buster (MGB) software for O-type stars and the IACOB standards catalog for B-type stars. Results: From the whole sample of 61 candidates, we have classified 42 stars as new massive OB-type stars, earlier than B3, in Cygnus OB2 and surroundings, including 11 O-type stars. The other candidates are discarded as they display later spectral types inconsistent with membership in the association. We have also obtained visual extinctions for all the new confirmed massive OB members, placing them in a Hertzsprung-Russell Diagram using calibrations for Teff and luminosity. Finally, we have studied the age and extinction distribution of our sample within the region. Conclusions: We have obtained new blue intermediate-resolution spectra suitable for spectral classification of 61 OB candidates in Cygnus OB2 and surroundings. The confirmation of 42 new OB massive stars (earlier than B3) in the region allows us to increase the young massive population known in the field. We have also confirmed the correlation between age and Galactic longitude previously found in the region. We conclude that many O and early B stars at B > 16 mag are still undiscovered in Cygnus.
The role of gap junctions in megakaryocyte-mediated osteoblast proliferation and differentiation.
Ciovacco, Wendy A; Goldberg, Carolyn G; Taylor, Amanda F; Lemieux, Justin M; Horowitz, Mark C; Donahue, Henry J; Kacena, Melissa A
2009-01-01
Gap junctions (GJs) are membrane-spanning channels that facilitate intercellular communication by allowing small signaling molecules (e.g. calcium ions, inositol phosphates, and cyclic nucleotides) to pass from cell to cell. Over the past two decades, many studies have described a role for GJ intercellular communication (GJIC) in the proliferation and differentiation of many cells, including bone cells. Recently, we reported that megakaryocytes (MKs) enhance osteoblast (OB) proliferation by a juxtacrine signaling mechanism. Here we determine whether this response is facilitated by GJIC. First we demonstrate that MKs express connexin 43 (Cx43), the predominant GJ protein expressed by bone cells, including OBs. Next, we provide data showing that MKs can communicate with OBs via GJIC, and that the addition of two distinct GJ uncouplers, 18alpha-glycyrrhetinic acid (alphaGA) or oleamide, inhibits this communication. We then demonstrate that inhibiting MK-mediated GJIC further enhances the ability of MKs to stimulate OB proliferation. Finally, we show that while culturing MKs with OBs reduces gene expression of several differentiation markers/matrix proteins (type I collagen, osteocalcin, and alkaline phosphatase), reduces alkaline phosphatase enzymatic activity, and decreases mineralization in OBs, blocking GJIC does not result in MK-induced reductions in OB gene expression, enzymatic levels, or mineralized nodule formation. Overall, these data provide evidence that GJIC between MKs and OBs is functional, and that inhibiting GJIC in MK-OB cultures enhances OB proliferation without apparently altering differentiation when compared to similarly treated OB cultures. Thus, these observations regarding MK-OB GJIC inhibition may provide insight regarding potential novel targets for anabolic bone formation.
The Role of Gap Junctions in Megakaryocyte-Mediated Osteoblast Proliferation and Differentiation
Ciovacco, Wendy A.; Goldberg, Carolyn G.; Taylor, Amanda F.; Lemieux, Justin M.; Horowitz, Mark C.; Donahue, Henry J.; Kacena, Melissa A.
2009-01-01
Gap junctions (GJs) are membrane-spanning channels that facilitate intercellular communication by allowing small signaling molecules (e.g. calcium ions, inositol phosphates, and cyclic nucleotides) to pass from cell to cell. Over the past two decades, many studies have described a role for GJ intercellular communication (GJIC) in the proliferation and differentiation of many cells, including bone cells. Recently, we reported that megakaryocytes (MKs) enhance osteoblast (OB) proliferation by a juxtacrine signaling mechanism. Here we determine whether that response is facilitated by GJIC. First we demonstrate that MKs express connexin 43 (Cx43), the predominant GJ protein expressed by bone cells, including OBs. Next, we provide data showing that MKs can communicate with OBs via GJIC, and that the addition of two distinct GJ uncouplers, 18α-glycyrrhetinic acid (αGA) or oleamide, inhibits this communication. We then demonstrate that inhibiting MK-mediated GJIC further enhances the ability of MK to stimulate OB proliferation. Finally, we show that while culturing MKs with OBs reduces gene expression of several differentiation markers/matrix proteins (type I collagen, osteocalcin, and alkaline phosphatase), reduces alkaline phosphatase enzymatic activity, and decreases mineralization in OBs, blocking GJIC does not result in MK-induced reductions in OB gene expression, enzymatic levels, or mineralized nodule formation. Overall, these data provide evidence that GJIC between MKs and OBs is functional, and that inhibiting GJIC in MK-OB cultures enhances OB proliferation without apparently altering differentiation when compared to similarly treated OB cultures. Thus, these observations regarding MK-OB GJIC inhibition may provide insight regarding potential novel targets for anabolic bone formation. PMID:18848655
Jones, Katherine M; Carter, Michele M; Schulkin, Jay
2015-06-01
African American and Hispanic women are disproportionately affected by cardiovascular disease (CVD) and its many risk factors. Obstetrician-gynecologists (OB/GYNs) play an integral role in well-woman care and have a unique opportunity to provide CVD counseling and screening to these at-risk and underserved groups. To assess whether OB/GYN race/ethnicity and OB/GYN practices with increasing minority patient populations predicted differences in OB/GYNs' knowledge, attitudes, and practice patterns relevant to racial/ethnic disparities in CVD. This study also sought to determine provider and patient-related barriers to CVD care. A questionnaire on CVD was mailed to 273 members of The American College of Obstetricians and Gynecologists in March-July 2013. African American and Hispanic OB/GYNs and OB/GYN practices with increasing minority patient populations were more knowledgeable of CVD disparities. These OB/GYNs reported greater concern for minority women's CVD risk relative to White OB/GYNs. Overall, OB/GYNs appear less knowledgeable and concerned with Hispanics' increased CVD risk relative to African Americans'. The most commonly reported provider and patient-related barriers to CVD care were time constraints, patient nonadherence to treatment recommendations, and inadequate training. It is likely that minority OB/GYNs and those with practices with increasing minority patient populations have greater exposure to women at risk for CVD. Dissemination of educational information regarding Hispanic women's CVD risk profile may improve OB/GYN knowledge, counseling, and screening. Increased training in CVD and multicultural competency during medical school and residency should help OB/GYNs overcome what they report as primary barriers to CVD care.
Yue, Zhijian; Zhang, Yuhui; Wang, Laixing; Liu, Jianmin
2017-01-01
Vasculogenic mimicry (VM) was an important tumor blood supply to complement the endothelial cell-dependent angiogenesis, while leptin and receptor (ObR) involved in angiogenesis in glioblastoma has been reported on previous study, but the relationship between ObR expression and VM formation in human glioblastoma tissues, as well as their prognostic significance still remains unclear. In our study, we found that VM recognized by CD31-/PAS+ immunohistochemical staining in glioblastoma tissues showed a positive correlation with leptin expression (r = 0.58, P < 0.01), as well as ObR expression in glioblastoma tissues (r = 0.61, P < 0.01). Association of glial to mesenchymal transition (GMT)-related molecular with ObR expression and VM formation in glioblastoma tissues indicated that ObR-positive glioblastoma cells with GMT phenotype might be more likely to constitute VM, and co-expression of ObR and CD133 or Nestin to constitute the channel impliated that ObR-positive glioblastoma cells displayed glioblastoma stem cells (GSC) properties. Moreover, Kaplan–Meier statistical analysis showed that patients with more VM or ObR expression displayed poorer prognosis for overall survival times than patients with less expression (VMhigh vs. VMlow: P = 0.033; ObRhigh vs. ObRlow: P = 0.009). And ObR+ glioblastoma cells with GSC characteristic were mostly involved in VM formation, whereas a little part of cells were also related to microvascular density (MVD), which suggested that ObR was an important target for anticancer therapy, so further related studies were needed to improve glioblastoma treatment. PMID:28938545
Evaluation of physical properties of different digital intraoral sensors.
Al-Rawi, Wisam; Teich, Sorin
2013-09-01
Digital technologies provide clinically acceptable results comparable to traditional films while having other advantages such as the ability to store and manipulate images, immediate evaluation of the image diagnostic quality, possible reduction in patient radiation exposure, and so on. The purpose of this paper is to present the results of the evaluation of the physical design of eight CMOS digital intraoral sensors. Sensors tested included: XDR (Cyber Medical Imaging, Los Angeles, CA, USA), RVG 6100 (Carestream Dental LLC, Atlanta, GA, USA), Platinum (DEXIS LLC., Hatfield, PA, USA), CDR Elite (Schick Technologies, Long Island City, NY, USA), ProSensor (Planmeca, Helsinki, Finland), EVA (ImageWorks, Elmsford, NY, USA), XIOS Plus (Sirona, Bensheim, Germany), and GXS-700 (Gendex Dental Systems, Hatfield, PA, USA). The sensors were evaluated for cable configuration, connectivity interface, presence of back-scattering radiation shield, plate thickness, active sensor area, and comparing the active imaging area to the outside casing and to conventional radiographic films. There were variations among the physical design of different sensors. For most parameters tested, a lack of standardization exists in the industry. The results of this study revealed that these details are not always available through the material provided by the manufacturers and are often not advertised. For all sensor sizes, active imaging area was smaller compared with conventional films. There was no sensor in the group that had the best physical design. Data presented in this paper establishes a benchmark for comparing the physical design of digital intraoral sensors.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Hong, Tianzhen; Chen, Yixing; Belafi, Zsofia
Occupant behavior (OB) in buildings is a leading factor influencing energy use in buildings. Quantifying this influence requires the integration of OB models with building performance simulation (BPS). This study reviews approaches to representing and implementing OB models in today’s popular BPS programs, and discusses weaknesses and strengths of these approaches and key issues in integrating of OB models with BPS programs. Two of the key findings are: (1) a common data model is needed to standardize the representation of OB models, enabling their flexibility and exchange among BPS programs and user applications; the data model can be implemented usingmore » a standard syntax (e.g., in the form of XML schema), and (2) a modular software implementation of OB models, such as functional mock-up units for co-simulation, adopting the common data model, has advantages in providing a robust and interoperable integration with multiple BPS programs. Such common OB model representation and implementation approaches help standardize the input structures of OB models, enable collaborative development of a shared library of OB models, and allow for rapid and widespread integration of OB models with BPS programs to improve the simulation of occupant behavior and quantification of their impact on building performance.« less
Hong, Tianzhen; Chen, Yixing; Belafi, Zsofia; ...
2017-07-27
Occupant behavior (OB) in buildings is a leading factor influencing energy use in buildings. Quantifying this influence requires the integration of OB models with building performance simulation (BPS). This study reviews approaches to representing and implementing OB models in today’s popular BPS programs, and discusses weaknesses and strengths of these approaches and key issues in integrating of OB models with BPS programs. Two of the key findings are: (1) a common data model is needed to standardize the representation of OB models, enabling their flexibility and exchange among BPS programs and user applications; the data model can be implemented usingmore » a standard syntax (e.g., in the form of XML schema), and (2) a modular software implementation of OB models, such as functional mock-up units for co-simulation, adopting the common data model, has advantages in providing a robust and interoperable integration with multiple BPS programs. Such common OB model representation and implementation approaches help standardize the input structures of OB models, enable collaborative development of a shared library of OB models, and allow for rapid and widespread integration of OB models with BPS programs to improve the simulation of occupant behavior and quantification of their impact on building performance.« less
Neutralizing IL-17 Prevents Obliterative Bronchiolitis in Murine Orthotopic Lung Transplantation
Fan, Lin; Benson, Heather L.; Vittal, Ragini; Mickler, Elizabeth A.; Presson, Robin; Jo Fisher, Amanda; Cummings, Oscar W.; Heidler, Kathleen M.; Keller, Melissa R.; Burlingham, William J.; Wilkes, David S.
2011-01-01
Obliterative bronchiolitis (OB) is the key impediment to the long term survival of lung transplant recipients and the lack of a robust pre-clinical model precludes examining OB immunopathogenesis. In the current study, lungs from C57BL/10 H-2b mice that are MHC compatible, but minor histocompatability antigen incompatible, were transplanted into C57BL/6 mice. Histological features and cytokine profiles of OB were assessed. Moderate rejection (grade A3) developed by day 14, with evidence of OB at that time point. At 21 days, OB was present in 55% of grafts and moderate to severe rejection (Grade A3-A4) was present in all mice. At 28 days, OB was present in 44% of mice and severe rejection (Grade A4) was present in all. IL-17A, but not IL-17F, splenic mRNA transcripts and serum protein levels were increased only in mice that developed OB, whereas IL-10 transcripts and protein were increased only in non-OB mice. Neutralizing IL-17 prevented OB, down regulated acute rejection, and upregulated systemic IL-10. Collectively, these data show that transplantation of minor histoincompatible lungs from C57BL/10 mice into C57BL/6 mice results in a highly reproducible pre-clinical model of OB. In addition, these data indicate that neutralizing IL-17A or augmenting IL-10 could be therapeutic interventions to prevent OB. PMID:21521466
Kelly, Glenn; Simpson, Grahame K; Brown, Suzanne; Kremer, Peter; Gillett, Lauren
2017-05-23
The objectives were to test the properties, via a psychometric study, of the Overt Behaviour Scale-Self-Report (OBS-SR), a version of the OBS-Adult Scale developed to provide a client perspective on challenging behaviours after acquired brain injury. Study sample 1 consisted of 37 patients with primary brain tumour (PBT) and a family-member informant. Sample 2 consisted of 34 clients with other acquired brain injury (mixed brain injury, MBI) and a service-provider informant. Participants completed the OBS-SR (at two time points), and the Awareness Questionnaire (AQ) and Mayo Portland Adaptability Inventory-III (MPAI-III) once; informants completed the OBS-Adult and AQ once only. PBT-informant dyads displayed "good" levels of agreement (ICC 2,k = .74; OBS-SR global index). Although MBI-informant dyads displayed no agreement (ICC 2,k = .22; OBS-SR global index), the sub-group (17/29) rated by clinicians as having moderate to good levels of awareness displayed "fair" agreement (ICC 2,k = .58; OBS-SR global index). Convergent/divergent validity was demonstrated by significant correlations between OBS-SR subscales and MPAI-III subscales with behavioural content (coefficients in the range .36 -.61). Scores had good reliability across one week (ICC 2,k = .69). The OBS-SR took approximately 15 minutes to complete. It was concluded that the OBS-SR demonstrated acceptable reliability and validity, providing a useful resource in understanding clients' perspectives about their behaviour.
NASA Astrophysics Data System (ADS)
Seeling, S.; Buddenbaum, H.; Seeger, M.; Löhnertz, M.
2009-04-01
In this presentation we summarize our experience in the derivation of variables for identification of erosion and areas endagered of erosion from different remote sensing sensors. The field study is situated at the "Zemmer-Plateau" (north-east from Trier) and was undertaken to compare the ability of different, passive and active, remote sensing sensors to derive several process parameters of soil erosion in agricultural landscapes. Additionally the added value of sensor combinations was investigated. Backscatter of C-Band microwave instruments is known to be sensitive to soil roughness and surface soil moisture. If landuse and roughness is approximately constant, backscatter is mostly affected by temporal changes in soil moisture. For the test site multitemporal imagery from the ASAR and ERS2 sensors was available. For the identification of areas prone to waterlogging an approach based on principal component analysis was used. Multitemporal imagery from optical sensors like Landsat and SPOT HRV allow the assessment of slow changes within the landscape and annual changes of vegetation cover. We used Landsat imagery from 1975, 1984 and 2000 to map the changes in landuse and associated soil development, multi temporal imagery from SPOT 4 and 5 satellites was used to identify different crop types. Additionally we investigated which areas that are prone to erosion by their topography position, have, due to maladjusted land management, not been protected by vegetation cover during the main annual rainfall season in 2003. Airborne Laser Scanning (ALS) data is well suited for discovering areas susceptible of erosion. Even under forest canopies ALS can provide high-resolution terrain models that can be used for identifying trenches, linear features, steep hills and other terrain features, which trigger erosion or are even results of erosion. ALS-derived DTMs usually have a spatial resolution of about 1 m, while DTMs from other data sources are much coarser. A key problem when working with ALS is finding the echoes that have really been reflected by the ground and not by buildings or vegetation. This is achieved by filtering the last and only return laser points. The investigations were aided be the analyses of two Quickbird datasets. The information layers derived from different sensors were merged into a preliminary erosion information system. This data base allows the identification of areas prone to erosion risk. Furthermore the results allow setting the focus on the most effective methods for further investigations.
Chitteti, Brahmananda R.; Cheng, Ying-Hua; Kacena, Melissa A.; Srour, Edward F.
2013-01-01
The role of osteoblasts (OB) in maintaining hematopoietic stem cells (HSC) in their niche is well elucidated, but the exact definition, both phenotypically and hierarchically of OB responsible for these functions is not clearly known. We previously demonstrated that OB maturational status influences HSC function whereby immature OB with high Runx2 expression promote hematopoietic expansion. Here, we show that Activated Leukocyte Cell Adhesion Molecule (ALCAM) or CD166 expression on OB is directly correlated with Runx2 expression and high hematopoiesis enhancing activity (HEA). Fractionation of OB with lineage markers: Sca1, osteopontin (OPN), CD166, CD44, and CD90 revealed that Lin-Sca1-OPN+CD166+ cells (CD166+) and their subpopulations fractionated with CD44 and CD90 expressed high levels of Runx2 and low levels of osteocalcin (OC) demonstrating the relatively immature status of these cells. Conversely, the majority of the Lin-Sca1-OPN+CD166− cells (CD166−) expressed high OC levels suggesting that CD166− OB are more mature. In vitro hematopoietic potential of LSK cells co-cultured for 7 days with fresh OB or OB pre-cultured for 1, 2, or 3 weeks declined precipitously with increasing culture duration concomitant with loss of CD166 expression. Importantly, LSK cells co-cultured with CD166+CD44+CD90+ OB maintained their in vivo repopulating potential through primary and secondary transplantation, suggesting that robust HEA activity is best mediated by immature CD166+ OB with high Runx2 and low OC expression. These studies begin to define the hierarchical organization of osteoblastic cells and provide a more refined definition of OB that can mediate HEA. PMID:23369988
Hasegawa, Tadashi; Ito, Yoshiya; Wijeweera, Jayanthika; Liu, Jie; Malle, Ernst; Farhood, Anwar; McCuskey, Robert S.; Jaeschke, Hartmut
2016-01-01
Steatosis is a major risk factor for complications after liver surgery. Since neutrophil cytotoxicity is critical for ischemia-reperfusion injury in normal livers, the aim of the present study was to evaluate whether an exaggerated inflammatory response could cause the increased injury in steatotic livers. In C57Bl/6 mice, 60 min of warm hepatic ischemia triggered a gradual increase in hepatic neutrophil accumulation during reperfusion with peak levels of 100-fold over baseline at 12 h of reperfusion. Neutrophil extravasation and a specific neutrophil-induced oxidant stress (immunostaining for hypochlorous acid-modified epitopes) started at 6 h of reperfusion and peaked at 12–24 h. Ob/ob mice, which had a severe macrovesicular steatosis, suffered significantly higher injury (alanine transaminase activity: 18,000 ± 2,100 U/l; 65% necrosis) compared with lean littermates (alanine transaminase activity: 4,900 ± 720 U/l; 24% necrosis) at 6 h of reperfusion. However, 62% fewer neutrophils accumulated in steatotic livers. This correlated with an attenuated increase in mRNA levels of several proinflammatory genes in ob/ob mice during reperfusion. In contrast, sham-operated ob/ob mice had a 50% reduction in liver blood flow and 35% fewer functional sinusoids compared with lean littermates. These deficiencies in liver blood flow and the microcirculation were further aggravated only in ob/ob mice during reperfusion. The attenuated inflammatory response and reduced neutrophil-induced oxidant stress observed in steatotic livers during reperfusion cannot be responsible for the dramatically increased injury in ob/ob mice. In contrast, the aggravated injury appears to be mediated by ischemic necrosis due to massive impairment of blood and oxygen supply in the steatotic livers. PMID:17307725
Salmon, D. Michael W.; Hems, Douglas A.
1973-01-01
1. Lipoproteins in the plasma of mice were characterized by agarose-gel chromatography and polyacrylamide-gel electrophoresis: genetically obese (ob/ob) mice exhibited hyperlipoproteinaemia (compared with lean mice), largely owing to an increase in the concentration of cholesterol in high-density lipoprotein. Plasma concentrations of triglyceride and phospholipid were not markedly increased in genetically obese mice. 2. The formation of glycerolipids in liver and plasma was investigated with 14C-labelled precursors. The synthesis of hepatic triglyceride and phospholipid from glucose or palmitate was enhanced in ob/ob mice, compared with lean mice. The rate of entry of triglyceride into plasma, calculated from the time-course of incorporation of 14C from [14C]palmitate into plasma triglyceride, was increased in ob/ob mice (0.5μmol of fatty acid/min, compared with 0.2 in lean mice). 3. The removal from plasma of murine lipoprotein triglyceride-[14C]fatty acid was increased in ob/ob mice (half-time 2.2min, compared with 7.2min in lean mice). Similar results were obtained with an injected lipid emulsion (Intralipid). 4. From these measurements, estimates of the rates of turnover of plasma triglyceride in mice (fed on a mixed diet, female, 3 months old) are about 1.0μmol of fatty acid/min in ob/ob mice, and 0.25 in lean mice. 5. The major precursor of hepatic and plasma triglyceride in lean and ob/ob mice was calculated to be plasma free fatty acid. 6. These results are discussed, in connexion with the role of the liver in triglyceride metabolism in mice, especially in relation to genetic obesity. PMID:4360712
Li, Zhiping; Oben, Jude A; Yang, Shiqi; Lin, Huizhi; Stafford, Elizabeth A; Soloski, Mark J; Thomas, Steven A; Diehl, Anna Mae
2004-08-01
It is not known why natural killer T (NKT) cells, which modulate liver injury by regulating local cytokine production, are reduced in leptin-deficient ob/ob mice. NKT cells express adrenoceptors. Thus, we hypothesize that the low norepinephrine (NE) activity of ob/ob mice promotes depletion of liver NKT cells, thereby sensitizing ob/ob livers to lipopolysaccharide (LPS) toxicity. To evaluate this hypothesis, hepatic NKT cells were quantified in wild-type mice before and after treatment with NE inhibitors, and in dopamine beta-hydroxylase knockout mice (which cannot synthesize NE) and ob/ob mice before and after 4 weeks of NE supplementation. Decreasing NE activity consistently reduces liver NKT cells, while increasing NE has the opposite effect. Analysis of hepatic and thymic NKT cells in mice of different ages demonstrate an age-related accumulation of hepatic NKT cells in normal mice, while liver NKT cells become depleted after birth in ob/ob mice, which have increased apoptosis of hepatic NKT cells. NE treatment inhibits apoptosis and restores hepatic NKT cells. In ob/ob mice with reduced hepatic NKT cells, hepatic T and NKT cells produce excessive T helper (Th)-1 proinflammatory cytokines and the liver is sensitized to LPS toxicity. NE treatment decreases Th-1 cytokines, increases production of Th-2 cytokines, and reduces hepatotoxicity. Studies of CD1d-deficient mice, which lack the receptor required for NKT cell development, demonstrate that they are also unusually sensitive to LPS hepatotoxicity. In conclusion, low NE activity increases hepatic NKT cell apoptosis and depletes liver NKT cells, promoting proinflammatory polarization of hepatic cytokine production that sensitizes the liver to LPS toxicity. Copyright 2004 American Association for the Study of Liver Diseases
Shafrir, E; Trostler, N
1984-01-01
The low-insulin responding spiny mice (Acomys cahirinus), maintained on a 50% sucrose diet vs isocaloric regular diet, responded with an impressive increase in the activity of hepatic enzymes of glycolysis and lipogenesis and in hyperlipidemia. There was no hyperinsulinemia or hyperglycemia and spiny mice did not gain weight on sucrose due to loss of adipose tissue. Serum T3 levels rose 1.8 fold and the activity of the hepatic mitochondrial FAD-glycerol-3-phosphate oxidase became induced 2.6 fold representing the enhancement of multiple, T3-dependent, energy-consuming metabolic cycles. An increased TG lipolysis in adipose tissue was also observed. C57BL/6J ob/ob mice were markedly hyperinsulinemic and gained weight on sucrose almost as much as those on regular diet, without changes in serum glucose or insulin. Serum triglyceride level decreased, whereas liver triglycerides accumulated markedly. The extent of the increase in hepatic enzyme activities related to lipogenesis was much lower both in the ob/ob mice and their lean siblings, than in spiny mice, but the basal enzyme activities in ob/ob mice were remarkably elevated. Serum T3 level was also elevated already on the regular diet and rose only slightly on sucrose. Basal glycerol phosphate oxidase activity in ob/ob mice exceeded that in spiny mice and rose only marginally on sucrose. Adipose tissue lipolysis was not increased. Thus, sucrose diet by enhancing the T3 production appeared to activate protective mechanism against weight gain in normoinsulinemic spiny mice, whereas the full expression of these mechanisms appeared to be precluded by the hyperinsulinemia of ob/ob mice.(ABSTRACT TRUNCATED AT 250 WORDS)
In vivo evidence for unidentified leptin-induced circulating factors that control white fat mass.
Harris, Ruth B S
2015-12-15
Fat transplants increase body fat mass without changing the energy status of an animal and provide a tool for investigating control of total body fat. Early transplant studies found that small pieces of transplanted fat took on the morphology of the transplant recipient. Experiments described here tested whether this response was dependent upon expression of leptin receptors in either transplanted fat or the recipient mouse. Fat from leptin receptor deficient db/db mice or wild-type mice was placed subcutaneously in db/db mice. After 12 wk, cell size distribution in the transplant was the same as in endogenous fat of the recipient. Thus, wild-type fat cells, which express leptin receptors, were enlarged in a hyperleptinemic environment, indicating that leptin does not directly control adipocyte size. By contrast, db/db or wild-type fat transplanted into wild-type mice decreased in size, suggesting that a functional leptin system in the recipient is required for body fat mass to be controlled. In the final experiment, wild-type fat was transplanted into a db/db mouse parabiosed to either another db/db mouse to an ob/ob mouse or in control pairs in which both parabionts were ob/ob mice. Transplants increased in size in db/db-db/db pairs, decreased in db/db-ob/ob pairs and did not change in ob/ob-ob/ob pairs. We propose that leptin from db/db parabionts activated leptin receptors in their ob/ob partners. This, in turn, stimulated release of unidentified circulating factors, which travelled back to the db/db partner and acted on the transplant to reduce fat cell size. Copyright © 2015 the American Physiological Society.
Mao, Xiangbing; Zeng, Xiangfang; Huang, Zhimin; Wang, Junjun; Qiao, Shiyan
2013-07-28
Leucine and leptin play important roles in regulating protein synthesis and degradation in skeletal muscles in vitro and in vivo. However, the objective of the present study was to determine whether leptin and leucine function synergistically in regulating protein metabolism of skeletal muscles. In the in vitro experiment, C2C12 myotubes were cultured for 2 h in the presence of 5 mm-leucine and/or 50 ng/ml of leptin. In the in vivo experiment, C57BL/6 and ob/ob mice were randomly assigned to be fed a non-purified diet supplemented with 3 % L-leucine or 2·04 % L-alanine (isonitrogenous control) for 14 d. Ob/ob mice were injected intraperitoneally with sterile PBS or recombinant mouse leptin (0·1 μg/g body weight) for 14 d. In C57BL/6 mice, dietary leucine supplementation increased (P< 0·05) plasma leptin, leptin receptor expression and protein synthesis in skeletal muscles, but reduced (P< 0·05) plasma urea and protein degradation in skeletal muscles. Dietary leucine supplementation and leptin injection increased the relative weight of the gastrocnemius and soleus muscles in ob/ob mice. Moreover, leucine and leptin treatments stimulated (P< 0·05) protein synthesis and inhibited (P< 0·05) protein degradation in C2C12 myotubes and skeletal muscles of ob/ob mice. There were interactions (P< 0·05) between the leucine and leptin treatments with regard to protein metabolism in C2C12 myotubes and soleus muscles of ob/ob mice but not in the gastrocnemius muscles of ob/ob mice. Collectively, these results suggest that leptin and leucine synergistically regulate protein metabolism in skeletal muscles both in vitro and in vivo.
Diversity in olfactory bulb size in birds reflects allometry, ecology, and phylogeny
Corfield, Jeremy R.; Price, Kasandra; Iwaniuk, Andrew N.; Gutierrez-Ibañez, Cristian; Birkhead, Tim; Wylie, Douglas R.
2015-01-01
The relative size of olfactory bulbs (OBs) is correlated with olfactory capabilities across vertebrates and is widely used to assess the relative importance of olfaction to a species’ ecology. In birds, variations in the relative size of OBs are correlated with some behaviors; however, the factors that have led to the high level of diversity seen in OB sizes across birds are still not well understood. In this study, we use the relative size of OBs as a neuroanatomical proxy for olfactory capabilities in 135 species of birds, representing 21 orders. We examine the scaling of OBs with brain size across avian orders, determine likely ancestral states and test for correlations between OB sizes and habitat, ecology, and behavior. The size of avian OBs varied with the size of the brain and this allometric relationship was for the most part isometric, although species did deviate from this trend. Large OBs were characteristic of more basal species and in more recently derived species the OBs were small. Living and foraging in a semi-aquatic environment was the strongest variable driving the evolution of large OBs in birds; olfaction may provide cues for navigation and foraging in this otherwise featureless environment. Some of the diversity in OB sizes was also undoubtedly due to differences in migratory behavior, foraging strategies and social structure. In summary, relative OB size in birds reflect allometry, phylogeny and behavior in ways that parallel that of other vertebrate classes. This provides comparative evidence that supports recent experimental studies into avian olfaction and suggests that olfaction is an important sensory modality for all avian species. PMID:26283931
Huh, Jeong-Eun; Seo, Dong-Min; Baek, Yong-Hyeon; Choi, Do-Young; Park, Dong-Suk; Lee, Jae-Dong
2010-04-01
Osteoarthritis is a multifactorial disease characterized by loss of articular cartilage and subchondral plate thickening. Therefore, biochemical analysis of the underlying bone tissue has provided important information for treatment of osteoarthritis. In this study, we determined the potential role of formononetin, a phytoestrogen isolated from Astragalus membranaceus to alter the expression of metabolic markers and cytokine production of human normal osteoblasts (Obs) and osteoarthritis subchondral osteoblasts (OA Obs). Human OA Obs and normal Obs were cultured for 3days, 7days or 14days in the present medium only or were treated with various doses of formononetin. Cells were analyzed for viability by WST-8 assay, alkaline phosphatase (ALP) activity, osteogenic markers (osteocalcin (OCN) and type I collagen (Col I)) and cytokines (interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2)). The level of IL-6, VEGF, BMP-2, OCN and Col I was increased in OA Obs compared with normal Obs. Formononetin dose-dependently decreased ALP, IL-6, VEGF, BMP-2, OCN and Col I in OA Obs, while markedly increased ALP, VEGF, BMP-2, OCN and Col I in normal Obs. Interestingly, formononetin markedly increased the expression of VEGF and BMP-2 for 3days of culture and significantly increased OCN and Col I at 14days in human normal Obs. The remodeling effect of formononetin on osteogenic markers and cytokines of inflammatory mediators was more striking in OA Obs as well. Taken together, these results could suggest that formononetin has biphasic positive effects on normal Obs and OA Obs by modifying their biological synthetic capacities. Crown Copyright 2010. Published by Elsevier B.V. All rights reserved.
Impurity-doped optical shock, detonation and damage location sensor
Weiss, J.D.
1995-02-07
A shock, detonation, and damage location sensor providing continuous fiber-optic means of measuring shock speed and damage location, and could be designed through proper cabling to have virtually any desired crush pressure. The sensor has one or a plurality of parallel multimode optical fibers, or a singlemode fiber core, surrounded by an elongated cladding, doped along their entire length with impurities to fluoresce in response to light at a different wavelength entering one end of the fiber(s). The length of a fiber would be continuously shorted as it is progressively destroyed by a shock wave traveling parallel to its axis. The resulting backscattered and shifted light would eventually enter a detector and be converted into a proportional electrical signals which would be evaluated to determine shock velocity and damage location. The corresponding reduction in output, because of the shortening of the optical fibers, is used as it is received to determine the velocity and position of the shock front as a function of time. As a damage location sensor the sensor fiber cracks along with the structure to which it is mounted. The size of the resulting drop in detector output is indicative of the location of the crack. 8 figs.
Impurity-doped optical shock, detonation and damage location sensor
Weiss, Jonathan D.
1995-01-01
A shock, detonation, and damage location sensor providing continuous fiber-optic means of measuring shock speed and damage location, and could be designed through proper cabling to have virtually any desired crush pressure. The sensor has one or a plurality of parallel multimode optical fibers, or a singlemode fiber core, surrounded by an elongated cladding, doped along their entire length with impurities to fluoresce in response to light at a different wavelength entering one end of the fiber(s). The length of a fiber would be continuously shorted as it is progressively destroyed by a shock wave traveling parallel to its axis. The resulting backscattered and shifted light would eventually enter a detector and be converted into a proportional electrical signals which would be evaluated to determine shock velocity and damage location. The corresponding reduction in output, because of the shortening of the optical fibers, is used as it is received to determine the velocity and position of the shock front as a function of time. As a damage location sensor the sensor fiber cracks along with the structure to which it is mounted. The size of the resulting drop in detector output is indicative of the location of the crack.
Large-scale magnetic topologies of mid M dwarfs
NASA Astrophysics Data System (ADS)
Morin, J.; Donati, J.-F.; Petit, P.; Delfosse, X.; Forveille, T.; Albert, L.; Aurière, M.; Cabanac, R.; Dintrans, B.; Fares, R.; Gastine, T.; Jardine, M. M.; Lignières, F.; Paletou, F.; Ramirez Velez, J. C.; Théado, S.
2008-10-01
We present in this paper, the first results of a spectropolarimetric analysis of a small sample (~20) of active stars ranging from spectral type M0 to M8, which are either fully convective or possess a very small radiative core. This study aims at providing new constraints on dynamo processes in fully convective stars. This paper focuses on five stars of spectral type ~M4, i.e. with masses close to the full convection threshold (~=0.35Msolar), and with short rotational periods. Tomographic imaging techniques allow us to reconstruct the surface magnetic topologies from the rotationally modulated time-series of circularly polarized profiles. We find that all stars host mainly axisymmetric large-scale poloidal fields. Three stars were observed at two different epochs separated by ~1 yr; we find the magnetic topologies to be globally stable on this time-scale. We also provide an accurate estimation of the rotational period of all stars, thus allowing us to start studying how rotation impacts the large-scale magnetic field. Based on observations obtained at the Canada-France-Hawaii Telescope (CFHT) and the Télescope Bernard Lyot (TBL). CFHT is operated by the National Research Council of Canada, the Institut National des Science de l'Univers of the Centre National de la Recherche Scientifique of France (INSU/CNRS) and the University of Hawaii, while the TBL is operated by CNRS/INSU. E-mail: jmorin@ast.obs-mip.fr (JM); donati@ast.obs-mip.fr (J-FD); petit@ast.obs-mip.fr (PP); xavier.delfosse@obs.ujf-grenoble.fr (XD); thierry.forveille@obs.ujf-grenoble.fr (TF); albert@cfht.hawaii.edu (LA); auriere@ast.obs-mip.fr (MA); remi.cabanac@ast.obs-mip.fr (RC); dintrans@ast.obs-mip.fr (BD); rfares@ast.obs-mip.fr (RF); tgastine@ast.obs-mip.fr (TG); mmj@st-andrews.ac.uk (MMJ); ligniere@ast.obs-mip.fr (FL); fpaletou@ast.obs-mip.fr (FP); jramirez@mesiog.obspm.fr (JR); sylvie.theado@ast.obs-mip.fr (ST)
Anderson, Brian M; Jacobson, Lauren; Novakovic, Zachary M; Grasso, Patricia
2017-06-01
This study describes the localization of [D-Leu-4]-OB3 and MA-[D-Leu-4]-OB3, synthetic peptide leptin mimetics, in the hypothalamus of Swiss Webster and C57BL/6J wild-type mice, leptin-deficient ob/ob mice, and leptin-resistant diet-induced obese (DIO) mice. The mice were given [D-Leu-4]-OB3 or MA-[D-Leu-4]-OB3 in 0.3% dodecyl maltoside by oral gavage. Once peak serum concentrations were reached, the mice received a lethal dose of pentobarbital and were subjected to intracardiac perfusion fixation. The brains were excised, post-fixed in paraformaldehyde, and cryo-protected in sucrose. Free-floating frozen coronal sections were cut at 25-µm and processed for imaging by immunofluorescence microscopy. In all four strains of mice, dense staining was concentrated in the area of the median eminence, at the base and/or along the inner wall of the third ventricle, and in the brain parenchyma at the level of the arcuate nucleus. These results indicate that [D-Leu-4]-OB3 and MA-[D-Leu-4]-OB3 cross the blood-brain barrier and concentrate in an area of the hypothalamus known to regulate energy balance and glucose homeostasis. Most noteworthy is the localization of [D-Leu-4]-OB3 immunoreactivity within the hypothalamus of DIO mice via a conduit that is closed to leptin in this rodent model, and in most cases of human obesity. Together with our previous studies describing the effects of [D-Leu-4]-OB3 and MA-[D-Leu-4]-OB3 on energy balance, glucose regulation, and signal transduction pathway activation, these findings are consistent with a central mechanism of action for these synthetic peptide leptin mimetics, and suggest their potential usefulness in the management of leptin-resistant obesity and type 2 diabetes in humans. Copyright © 2017 Elsevier B.V. All rights reserved.
An NFC-Enabled CMOS IC for a Wireless Fully Implantable Glucose Sensor.
DeHennis, Andrew; Getzlaff, Stefan; Grice, David; Mailand, Marko
2016-01-01
This paper presents an integrated circuit (IC) that merges integrated optical and temperature transducers, optical interface circuitry, and a near-field communication (NFC)-enabled digital, wireless readout for a fully passive implantable sensor platform to measure glucose in people with diabetes. A flip-chip mounted LED and monolithically integrated photodiodes serve as the transduction front-end to enable fluorescence readout. A wide-range programmable transimpedance amplifier adapts the sensor signals to the input of an 11-bit analog-to-digital converter digitizing the measurements. Measurement readout is enabled by means of wireless backscatter modulation to a remote NFC reader. The system is able to resolve current levels of less than 10 pA with a single fluorescent measurement energy consumption of less than 1 μJ. The wireless IC is fabricated in a 0.6-μm-CMOS process and utilizes a 13.56-MHz-based ISO15693 for passive wireless readout through a NFC interface. The IC is utilized as the core interface to a fluorescent, glucose transducer to enable a fully implantable sensor-based continuous glucose monitoring system.
Olfactory bulb volume in Taiwanese patients with posttraumatic anosmia.
Jiang, Rong-San; Chai, Jyh-Wen; Chen, Wen-Hsien; Fuh, Wen-Bin; Chiang, Chin-Ming; Chen, Clayton Chi-Chang
2009-01-01
Olfactory bulb (OB) volume has been shown to be an indicator of olfactory function. However, few studies have been done in Asia to investigate the influence of different disorders on OB volume. Data from patients with posttraumatic anosmia were collected in our department. Their olfactory thresholds were assessed by the phenyl ethyl alcohol threshold test. They were treated with a course of high-dose steroid, and followed up for at least 3 months without any olfactory improvement. Magnetic resonance imaging was subsequently used to measure patients' OB volumes. Subjects who self-reported their olfactory function was normal were also included in the control group for comparison. Fifty-four patients with posttraumatic anosmia and 30 subjects who self-reported their olfactory function was normal were enrolled in this study. The mean right OB volume was 45.2 mm3, and the mean left OB volume was 46.3 mm3 in patients with posttraumatic anosmia. The mean right OB volume was 59.7 mm3, and the mean left OB volume was 66.0 mm3 in control subjects. The OB volumes were significantly lower in patients with posttraumatic anosmia. OB volumes were significantly lower in Taiwanese patients with posttraumatic anosmia.
Strong, Amy L; Ohlstein, Jason F; Biagas, Brandi A; Rhodes, Lyndsay V; Pei, Dorothy T; Tucker, H Alan; Llamas, Claire; Bowles, Annie C; Dutreil, Maria F; Zhang, Shijia; Gimble, Jeffrey M; Burow, Matthew E; Bunnell, Bruce A
2015-08-19
The steady increase in the incidence of obesity among adults has been paralleled with higher levels of obesity-associated breast cancer. While recent studies have suggested that adipose stromal/stem cells (ASCs) isolated from obese women enhance tumorigenicity, the mechanism(s) by which this occurs remains undefined. Evidence suggests that increased adiposity results in increased leptin secretion from adipose tissue, which has been shown to increased cancer cell proliferation. Previously, our group demonstrated that ASCs isolated from obese women (obASCs) also express higher levels of leptin relative to ASCs isolated from lean women (lnASCs) and that this obASC-derived leptin may account for enhanced breast cancer cell growth. The current study investigates the impact of inhibiting leptin expression in lnASCs and obASCs on breast cancer cell (BCC) growth and progression. Estrogen receptor positive (ER+) BCCs were co-cultured with leptin shRNA lnASCs or leptin shRNA obASCs and changes in the proliferation, migration, invasion, and gene expression of BCCs were investigated. To assess the direct impact of leptin inhibition in obASCs on BCC proliferation, MCF7 cells were injected alone or mixed with control shRNA obASCs or leptin shRNA obASCs into SCID/beige mice. ER+ BCCs were responsive to obASCs during direct co-culture, whereas lnASCs were unable to increase ER(+) BCC growth. shRNA silencing of leptin in obASCs negated the enhanced proliferative effects of obASC on BCCs following direct co-culture. BCCs co-cultured with obASCs demonstrated enhanced expression of epithelial-to-mesenchymal transition (EMT) and metastasis genes (SERPINE1, MMP-2, and IL-6), while BCCs co-cultured with leptin shRNA obASCs did not display similar levels of gene induction. Knockdown of leptin significantly reduced tumor volume and decreased the number of metastatic lesions to the lung and liver. These results correlated with reduced expression of both SERPINE1 and MMP-2 in tumors formed with MCF7 cells mixed with leptin shRNA obASCs, when compared to tumors formed with MCF7 cells mixed with control shRNA obASCs. This study provides mechanistic insight as to how obesity enhances the proliferation and metastasis of breast cancer cells; specifically, obASC-derived leptin contributes to the aggressiveness of breast cancer in obese women.
AVIRIS calibration using the cloud-shadow method
NASA Technical Reports Server (NTRS)
Carder, K. L.; Reinersman, P.; Chen, R. F.
1993-01-01
More than 90 percent of the signal at an ocean-viewing, satellite sensor is due to the atmosphere, so a 5 percent sensor-calibration error viewing a target that contributes but 10 percent of the signal received at the sensor may result in a target-reflectance error of more than 50 percent. Since prelaunch calibration accuracies of 5 percent are typical of space-sensor requirements, recalibration of the sensor using ground-base methods is required for low-signal target. Known target reflectance or water-leaving radiance spectra and atmospheric correction parameters are required. In this article we describe an atmospheric-correction method that uses cloud shadowed pixels in combination with pixels in a neighborhood region of similar optical properties to remove atmospheric effects from ocean scenes. These neighboring pixels can then be used as known reflectance targets for validation of the sensor calibration and atmospheric correction. The method uses the difference between water-leaving radiance values for these two regions. This allows nearly identical optical contributions to the two signals (e.g., path radiance and Fresnel-reflected skylight) to be removed, leaving mostly solar photons backscattered from beneath the sea to dominate the residual signal. Normalization by incident solar irradiance reaching the sea surface provides the remote-sensing reflectance of the ocean at the location of the neighbor region.
Wang, Anke; Anderson, Brian M; Novakovic, Zachary M; Grasso, Patricia
2018-03-01
We have previously shown that following oral delivery in dodecyl maltoside (DDM), [D-Leu-4]-OB3 and its myristic acid conjugate, MA-[D-Leu-4]-OB3, improved energy balance and glucose homeostasis in genetically obese/diabetic mouse models. More recently, we have provided immunohistochemical evidence indicating that these synthetic peptide leptin mimetics cross the blood-brain barrier and concentrate in the area of the arcuate nucleus of the hypothalamus in normal C57BL/6J and Swiss Webster mice, in genetically obese ob/ob mice, and in diet-induced obese (DIO) mice. In the present study, we describe the effects of oral delivery of [D-Leu-4]-OB3 and MA-[D-Leu-4]-OB3 on glycemic control in diet-induced (DIO) mice, a non-genetic rodent model of obesity and its associated insulin resistance, which more closely recapitulates common obesity and diabetes in humans. Male C57BL/6J and DIO mice, 17, 20, and 28 weeks of age, were maintained on a low-fat or high-fat diet and given vehicle (DDM) alone or [D-Leu-4]-OB3 or MA-[D-Leu-4]-OB3 in DDM by oral gavage for 12 or 14 days. Body weight gain, food and water intake, fasting blood glucose, oral glucose tolerance, and serum insulin levels were measured. Our data indicate that (1) [D-Leu-4]-OB3 and MA-[D-Leu-4]-OB3 restore glucose tolerance in male DIO mice maintained on a high-fat diet to levels comparable to those of non-obese C57BL/6J wild-type mice of the same age and sex maintained on a low-fat diet; and (2) the influence of [D-Leu-4]-OB3 and MA-[D-Leu-4]-OB3 on glycemic control appears to be independent of their effects on energy balance. These results suggest that [D-Leu-4]-OB3 and/or MA-[D-Leu-4]-OB3 may have application to the management of the majority of cases of common obesity in humans, a state characterized at least in part, by leptin resistance resulting from a defect in leptin transport across the blood-brain barrier. They further suggest that these small molecule synthetic peptide leptin mimetics, through their influence on glycemic control, may prevent the pre-diabetic state associated with most cases of common obesity from escalating into overt type 2 diabetes mellitus. Copyright © 2017 Elsevier Inc. All rights reserved.
Tanik, Nermin; Serin, Halil Ibrahim; Celikbilek, Asuman; Inan, Levent Ertugrul; Gundogdu, Fatma
2015-11-15
Multiple sclerosis (MS) is a neuroinflammatory and neurodegenerative disease that progresses to axonal loss and demyelinization. Olfactory dysfunction in patients with MS has been reported frequently. We were interested in the associations of olfactory bulb (OB) and olfactory sulcus depth (OSD) with disease duration and attack frequency. We included 25 patients with MS and 30 age- and sex-matched controls in this study. The Expanded Disability Status Scale, Beck Depression Inventory, and Mini Mental State Examination were applied. OB, OSD, and magnetic resonance imaging plaque numbers were calculated. OB volume and OSD in patients with MS were significantly lower than those in the control group (right and left OB: p<0.001; right OSD: p=0.001; and left OSD: p=0.039). Disease duration was negatively correlated with right and left OB volume (right OB: r=-0.434, p=0.030 and left OB: r=-0.518, p=0.008). Attack frequency was negatively correlated with left OB volume and left OSD (left OB: r=-0.428, p=0.033 and left OSD: r=-0.431, p=0.032). The OB and OSD were atrophied significantly in patients with MS, and this was correlated with disease duration and attack frequency. The left side tended to be dominant. Copyright © 2015 Elsevier B.V. All rights reserved.
Oil Bodies Extracted from High-Fat and Low-Fat Soybeans: Stability and Composition During Storage.
Wang, Qiu Ling; Li Cui, Chun; Jiang, Lian Zhou; Liu, Yue; Liang, Xin Ting; Hou, Jun Cai
2017-06-01
Soybeans contain oil bodies (OBs) that encapsulate triacylglycerols (TAGs) with a phospholipid monolayer carrying scattered proteins. In nature, soybean OBs can form natural emulsions in aqueous media and may serve as natural, minimally processed, stable, and pre-emulsified oil for addition into appropriate food systems. In this study, OBs were obtained by aqueous extraction from the mature seeds of 2 soybean crop cultivars, high-fat soybean and low-fat soybeans. The compositions of the extracted OBs were analyzed during storage at room temperature up to 14 d (pH = 7). The oxidative stability of these OBs, stored at 60 °C, was evaluated by measuring the presence of primary (lipid hydroperoxides) and secondary lipid oxidation products (malondialdehyde) by determining the standard peroxide value (PV) and thiobarbituric acid-reactive substances (TBARS) value. During storage, the contents of unsaturated fatty acids, phospholipids, and tocopherols declined in both OBs, while their mean particle diameters (d 32 ) and ζ-potentials increased. The changes in PV and TBARS values exhibited a similar trend for both OBs, but the OBs from low-fat soybeans had significantly lower PV and higher TBARS values than the OBs from high-fat soybean cultivars (P < 0.05). Overall, the OBs from both soybean cultivars had good stability during storage. © 2017 Institute of Food Technologists®.
A theoretical/experimental program to develop active optical pollution sensors, part 2
NASA Technical Reports Server (NTRS)
Poultney, S. K.
1975-01-01
Progress is reported on experimental investigations of Lidar and the application of Lidar to environmental and atmospheric science. Specifically the following programs are considered: calibration and application of the LaRC 48-inch Lidar; efficient and certain detection of SO2 and other gases in the calibration tank using the Raman Stack Monitor Lidar; the potential of Lidar remote sensing from the space shuttle; and the planning and mounting of efforts to realize the promise of backscatter differential absorption Lidar.
Dependence of Radar Backscatter on the Energetics of the Air-Sea Interface
1990-07-01
14 3 Figure 41a. Shematic Spectrum of Wind Speed Near the Ground Estimated from a Study of Van der Hoven (1957...O.O0 Figure 41a. Schematic Spectrum of Wind Speed Near the Ground Estimated from a Study of Van der Hoven (1957) (from Lumley and Panofsky, 1964) The...resolved is 0.6 to 8.0s. Following Der (1976), the sensors are capacitance transduction devices which produce output voltage signals proportional to surface
Surface Observation Climatic Summaries (SOCS) For Mather AFB, California
1992-02-01
1100 SPEND (RTS) CE14 GEIS GE25 OBS GE14 GEl8 GE25 OBS GE14 GEl8 GE25 OBS GE14 GEl8 GE25 OBS CATEGORY A 1.7 .5 .1 930 1.6 .6 .1 930 1.6 .4 930 3.4 1.0...ITS) GE14 0E18 GE25 ORS GE14 GEIS GE25 OS GE14 GEl8 GE25 OBS GE14 GE18 GE25 OBS CATEGORY A 5.7 2.4 930 5.2 1.3 .2 930 1.7 .5 .2 930 1.5 .5 .1 930...TIME (LST) 0600 - 2000 ALL HOURS SPEED K75 GE14 GEl8 GE25 OBS GE14 GEl8 UE25 OS CAT Y A 3.5 1.1 .1 4650 2.8 .9
Krisko, Tibor I; LeClair, Katherine B; Cohen, David E
2017-03-01
Phosphatidylcholine transfer protein (PC-TP; synonym StarD2) is highly expressed in liver and oxidative tissues. PC-TP promotes hepatic glucose production during fasting and aggravates glucose intolerance in high fat fed mice. However, because PC-TP also suppresses thermogenesis in brown adipose tissue (BAT), its direct contribution to obesity-associated diabetes in mice remains unclear. Here we examined the effects of genetic PC-TP ablation on glucose homeostasis in leptin-deficient ob/ob mice, which exhibit both diabetes and altered thermoregulation. Mice lacking both PC-TP and leptin (Pctp -/- ;ob/ob) were prepared by crossing Pctp -/- with ob/+ mice. Glucose homeostasis was assessed by standard assays, and energy expenditure was determined by indirect calorimetry using a comprehensive laboratory animal monitoring system, which also recorded physical activity and food intake. Body composition was determined by NMR and hepatic lipids by enzymatic assays. Core body temperature was measured using a rectal thermocouple probe. Pctp -/- ;ob/ob mice demonstrated improved glucose homeostasis, as evidenced by markedly improved glucose and pyruvate tolerance tests, without changes in insulin tolerance. However, there were no differences in EE at any ambient temperature. There were also no effects of PC-TP expression on physical activity, food intake or core body temperature. Improved glucose tolerance in Pctp -/- ;ob/ob mice in the absence of increases in energy expenditure or core body temperature indicates a direct pathogenic role for PC-TP in diabetes in leptin deficient mice. Copyright © 2016 Elsevier Inc. All rights reserved.
Ham, Suyun; Song, Homin; Oelze, Michael L; Popovics, John S
2017-03-01
We describe an approach that utilizes ultrasonic surface wave backscatter measurements to characterize the volume content of relatively small distributed defects (microcrack networks) in concrete. A simplified weak scattering model is used to demonstrate that the scattered wave field projected in the direction of the surface wave propagation is relatively insensitive to scatterers that are smaller than the propagating wavelength, while the scattered field projected in the opposite direction is more sensitive to sub-wavelength scatterers. Distributed microcracks in the concrete serve as the small scatterers that interact with a propagating surface wave. Data from a finite element simulation were used to demonstrate the viability of the proposed approach, and also to optimize a testing configuration to collect data. Simulations were validated through experimental measurements of ultrasonic backscattered surface waves from test samples of concrete constructed with different concentrations of fiber filler (0.0, 0.3 and 0.6%) to mimic increasing microcrack volume density and then samples with actual cracking induced by controlled thermal cycles. A surface wave was induced in the concrete samples by a 50kHz ultrasonic source operating 10mm above the surface at an angle of incidence of 9°. Silicon-based miniature MEMS acoustic sensors located a few millimeters above the concrete surface both behind and in front of the sender were used to detect leaky ultrasonic surface waves emanating from concrete. A normalized backscattered energy parameter was calculated from the signals. Statistically significant differences in the normalized backscattered energy were observed between concrete samples with varying levels of simulated and actual cracking damage volume. Copyright © 2016 Elsevier B.V. All rights reserved.
Model validation of untethered, ultrasonic neural dust motes for cortical recording.
Seo, Dongjin; Carmena, Jose M; Rabaey, Jan M; Maharbiz, Michel M; Alon, Elad
2015-04-15
A major hurdle in brain-machine interfaces (BMI) is the lack of an implantable neural interface system that remains viable for a substantial fraction of the user's lifetime. Recently, sub-mm implantable, wireless electromagnetic (EM) neural interfaces have been demonstrated in an effort to extend system longevity. However, EM systems do not scale down in size well due to the severe inefficiency of coupling radio-waves at those scales within tissue. This paper explores fundamental system design trade-offs as well as size, power, and bandwidth scaling limits of neural recording systems built from low-power electronics coupled with ultrasonic power delivery and backscatter communication. Such systems will require two fundamental technology innovations: (1) 10-100 μm scale, free-floating, independent sensor nodes, or neural dust, that detect and report local extracellular electrophysiological data via ultrasonic backscattering and (2) a sub-cranial ultrasonic interrogator that establishes power and communication links with the neural dust. We provide experimental verification that the predicted scaling effects follow theory; (127 μm)(3) neural dust motes immersed in water 3 cm from the interrogator couple with 0.002064% power transfer efficiency and 0.04246 ppm backscatter, resulting in a maximum received power of ∼0.5 μW with ∼1 nW of change in backscatter power with neural activity. The high efficiency of ultrasonic transmission can enable the scaling of the sensing nodes down to 10s of micrometer. We conclude with a brief discussion of the application of neural dust for both central and peripheral nervous system recordings, and perspectives on future research directions. Copyright © 2014 Elsevier B.V. All rights reserved.
Leptin differentially regulates STAT3 activation in the ob/ob mice adipose mesenchymal stem cells
USDA-ARS?s Scientific Manuscript database
Leptin-deficient genetically obese ob/ob mice exhibit adipocyte hypertrophy and hyperplasia as well as elevated adipose tissue and systemic inflammation. Studies have shown that multipotent stem cells isolated from adult adipose tissue can differentiate into adipocytes ex vivo and thereby contribute...
Ocean bottom seismometer: design and test of a measurement system for marine seismology.
Mànuel, Antoni; Roset, Xavier; Del Rio, Joaquin; Toma, Daniel Mihai; Carreras, Normandino; Panahi, Shahram Shariat; Garcia-Benadí, A; Owen, Tim; Cadena, Javier
2012-01-01
The Ocean Bottom Seismometer (OBS) is a key instrument for the geophysical study of sea sub-bottom layers. At present, more reliable autonomous instruments capable of recording underwater for long periods of time and therefore handling large data storage are needed. This paper presents a new Ocean Bottom Seismometer designed to be used in long duration seismic surveys. Power consumption and noise level of the acquisition system are the key points to optimize the autonomy and the data quality. To achieve our goals, a new low power data logger with high resolution and Signal-to-Noise Ratio (SNR) based on Compact Flash memory card is designed to enable continuous data acquisition. The equipment represents the achievement of joint work from different scientific and technological disciplines as electronics, mechanics, acoustics, communications, information technology, marine geophysics, etc. This easy to handle and sophisticated equipment allows the recording of useful controlled source and passive seismic data, as well as other time varying data, with multiple applications in marine environment research. We have been working on a series of prototypes for ten years to improve many of the aspects that make the equipment easy to handle and useful to work in deep-water areas. Ocean Bottom Seismometers (OBS) have received growing attention from the geoscience community during the last forty years. OBS sensors recording motion of the ocean floor hold key information in order to study offshore seismicity and to explore the Earth's crust. In a seismic survey, a series of OBSs are placed on the seabed of the area under study, where they record either natural seismic activity or acoustic signals generated by compressed air-guns on the ocean surface. The resulting data sets are subsequently used to model both the earthquake locations and the crustal structure.
Ocean Bottom Seismometer: Design and Test of a Measurement System for Marine Seismology
Mànuel, Antoni; Roset, Xavier; Del Rio, Joaquin; Toma, Daniel Mihai; Carreras, Normandino; Panahi, Shahram Shariat; Garcia-Benadí, A.; Owen, Tim; Cadena, Javier
2012-01-01
The Ocean Bottom Seismometer (OBS) is a key instrument for the geophysical study of sea sub-bottom layers. At present, more reliable autonomous instruments capable of recording underwater for long periods of time and therefore handling large data storage are needed. This paper presents a new Ocean Bottom Seismometer designed to be used in long duration seismic surveys. Power consumption and noise level of the acquisition system are the key points to optimize the autonomy and the data quality. To achieve our goals, a new low power data logger with high resolution and Signal–to-Noise Ratio (SNR) based on Compact Flash memory card is designed to enable continuous data acquisition. The equipment represents the achievement of joint work from different scientific and technological disciplines as electronics, mechanics, acoustics, communications, information technology, marine geophysics, etc. This easy to handle and sophisticated equipment allows the recording of useful controlled source and passive seismic data, as well as other time varying data, with multiple applications in marine environment research. We have been working on a series of prototypes for ten years to improve many of the aspects that make the equipment easy to handle and useful to work in deep-water areas. Ocean Bottom Seismometers (OBS) have received growing attention from the geoscience community during the last forty years. OBS sensors recording motion of the ocean floor hold key information in order to study offshore seismicity and to explore the Earth’s crust. In a seismic survey, a series of OBSs are placed on the seabed of the area under study, where they record either natural seismic activity or acoustic signals generated by compressed air-guns on the ocean surface. The resulting data sets are subsequently used to model both the earthquake locations and the crustal structure. PMID:22737032
Accurate ocean bottom seismometer positioning method inspired by multilateration technique
Benazzouz, Omar; Pinheiro, Luis M.; Matias, Luis M. A.; Afilhado, Alexandra; Herold, Daniel; Haines, Seth S.
2018-01-01
The positioning of ocean bottom seismometers (OBS) is a key step in the processing flow of OBS data, especially in the case of self popup types of OBS instruments. The use of first arrivals from airgun shots, rather than relying on the acoustic transponders mounted in the OBS, is becoming a trend and generally leads to more accurate positioning due to the statistics from a large number of shots. In this paper, a linearization of the OBS positioning problem via the multilateration technique is discussed. The discussed linear solution solves jointly for the average water layer velocity and the OBS position using only shot locations and first arrival times as input data.
Automotive System for Remote Surface Classification.
Bystrov, Aleksandr; Hoare, Edward; Tran, Thuy-Yung; Clarke, Nigel; Gashinova, Marina; Cherniakov, Mikhail
2017-04-01
In this paper we shall discuss a novel approach to road surface recognition, based on the analysis of backscattered microwave and ultrasonic signals. The novelty of our method is sonar and polarimetric radar data fusion, extraction of features for separate swathes of illuminated surface (segmentation), and using of multi-stage artificial neural network for surface classification. The developed system consists of 24 GHz radar and 40 kHz ultrasonic sensor. The features are extracted from backscattered signals and then the procedures of principal component analysis and supervised classification are applied to feature data. The special attention is paid to multi-stage artificial neural network which allows an overall increase in classification accuracy. The proposed technique was tested for recognition of a large number of real surfaces in different weather conditions with the average accuracy of correct classification of 95%. The obtained results thereby demonstrate that the use of proposed system architecture and statistical methods allow for reliable discrimination of various road surfaces in real conditions.
Automotive System for Remote Surface Classification
Bystrov, Aleksandr; Hoare, Edward; Tran, Thuy-Yung; Clarke, Nigel; Gashinova, Marina; Cherniakov, Mikhail
2017-01-01
In this paper we shall discuss a novel approach to road surface recognition, based on the analysis of backscattered microwave and ultrasonic signals. The novelty of our method is sonar and polarimetric radar data fusion, extraction of features for separate swathes of illuminated surface (segmentation), and using of multi-stage artificial neural network for surface classification. The developed system consists of 24 GHz radar and 40 kHz ultrasonic sensor. The features are extracted from backscattered signals and then the procedures of principal component analysis and supervised classification are applied to feature data. The special attention is paid to multi-stage artificial neural network which allows an overall increase in classification accuracy. The proposed technique was tested for recognition of a large number of real surfaces in different weather conditions with the average accuracy of correct classification of 95%. The obtained results thereby demonstrate that the use of proposed system architecture and statistical methods allow for reliable discrimination of various road surfaces in real conditions. PMID:28368297
NASA Technical Reports Server (NTRS)
Redemann, Jens; Russell, Philip B.; Winker, David M.; McCormick, M. Patrick; Hipskind, R. Stephen (Technical Monitor)
2000-01-01
The current low confidence in the estimates of aerosol-induced perturbations of Earth's radiation balance is caused by the highly non-uniform compositional, spatial and temporal distributions of tropospheric aerosols on a global scale owing to their heterogeneous sources and short lifetimes. Nevertheless, recent studies have shown that the inclusion of aerosol effects in climate model calculations can improve agreement with observed spatial and temporal temperature distributions. In light of the short lifetimes of aerosols, determination of their global distribution with space-borne sensors seems to be a necessary approach. Until recently, satellite measurements of tropospheric aerosols have been approximate and did not provide the full set of information required to determine their radiative effects. With the advent of active aerosol remote sensing from space (e.g., PICASSO-CENA), the applicability fo lidar-derived aerosol 180 deg -backscatter data to radiative flux calculations and hence studies of aerosol effects on climate needs to be investigated.
Automated identification of OB associations in M31
NASA Technical Reports Server (NTRS)
Magnier, Eugene A.; Battinelli, Paolo; Lewin, Walter H. G.; Haiman, Zoltan; Paradijs, Jan Van; Hasinger, Guenther; Pietsch, Wolfgang; Supper, Rodrigo; Truemper, Joachim
1993-01-01
A new identification of OB associations in M31 has been performed using the Path Linkage Criterion (PLC) technique of Battinelli (1991). We found 174 associations with a very small contamination (less than 5%) by random clumps of stars. The expected total number and average size of OB associations in the region of M 31 covered by our data set (Magnier et al. 1992) are approximately 280 and approximately 90 pc, respectively. M31 associations therefore have sizes similar to those of OB associations observed in nearby galaxies, so that we can consider them to be classical OB associations. This list of OB associations will be used for the study of the spatial distribution of OB associations and their correlation with other objects. Taking into account the fact that we do not cover the entire disk of M31, we extrapolate a total number of association in M31 of approximately 420.
Organic brain syndrome and aging. A six-year follow-up of surviving twins.
Jarvik, L F; Ruth, V; Matsuyama, S S
1980-03-01
The development of organic brain syndrome (OBS) was studied in a small group of survivors from a longitudinal investigation of aging twins. At the time of initial evaluation, the frequency of moderate to severe OBS was 25%. Among the 22 survivors who had a second psychiatric evaluation after approximately six years, the corrected rate for the development of OBS among those without it at the initial examination was 16%. Thus, the vast majority of those diagnosed as being without OBS at about the age of 80 years remained asymptomatic in subsequent years, supporting the view that OBS is not a necessary concomitant of old age, but the result of disease for which prevention and cure should be sought. Persons originally diagnosed as having OBS had the higher mortality, an observation in accord with prior reports in the literature. In the present study, the increased mortality was related to the severity of OBS but apparently independent of coexisting physical illness, again supporting the argument that OBS represents pathological as distinct from physiological aging.
Structural anatomy of telomere OB proteins.
Horvath, Martin P
2011-10-01
Telomere DNA-binding proteins protect the ends of chromosomes in eukaryotes. A subset of these proteins are constructed with one or more OB folds and bind with G+T-rich single-stranded DNA found at the extreme termini. The resulting DNA-OB protein complex interacts with other telomere components to coordinate critical telomere functions of DNA protection and DNA synthesis. While the first crystal and NMR structures readily explained protection of telomere ends, the picture of how single-stranded DNA becomes available to serve as primer and template for synthesis of new telomere DNA is only recently coming into focus. New structures of telomere OB fold proteins alongside insights from genetic and biochemical experiments have made significant contributions towards understanding how protein-binding OB proteins collaborate with DNA-binding OB proteins to recruit telomerase and DNA polymerase for telomere homeostasis. This review surveys telomere OB protein structures alongside highly comparable structures derived from replication protein A (RPA) components, with the goal of providing a molecular context for understanding telomere OB protein evolution and mechanism of action in protection and synthesis of telomere DNA.
Structural anatomy of telomere OB proteins
Horvath, Martin P.
2015-01-01
Telomere DNA-binding proteins protect the ends of chromosomes in eukaryotes. A subset of these proteins are constructed with one or more OB folds and bind with G+T-rich single-stranded DNA found at the extreme termini. The resulting DNA-OB protein complex interacts with other telomere components to coordinate critical telomere functions of DNA protection and DNA synthesis. While the first crystal and NMR structures readily explained protection of telomere ends, the picture of how single-stranded DNA becomes available to serve as primer and template for synthesis of new telomere DNA is only recently coming into focus. New structures of telomere OB fold proteins alongside insights from genetic and biochemical experiments have made significant contributions towards understanding how protein-binding OB proteins collaborate with DNA-binding OB proteins to recruit telomerase and DNA polymerase for telomere homeostasis. This review surveys telomere OB protein structures alongside highly comparable structures derived from replication protein A (RPA) components, with the goal of providing a molecular context for understanding telomere OB protein evolution and mechanism of action in protection and synthesis of telomere DNA. PMID:21950380
Ogura, Kasane; Ogura, Masahito; Shoji, Toshihiko; Sato, Yuichi; Tahara, Yumiko; Yamano, Gen; Sato, Hiroki; Sugizaki, Kazu; Fujita, Naotaka; Tatsuoka, Hisato; Usui, Ryota; Mukai, Eri; Fujimoto, Shimpei; Inagaki, Nobuya; Nagashima, Kazuaki
2016-11-23
Procyanidins, the main ingredient of apple polyphenols, are known to possess antioxidative and anti-inflammatory effects associated closely with the pathophysiology of insulin resistance and type 2 diabetes. We investigated the effects of orally administered apple procyanidins (APCs) on glucose metabolism using diabetic ob/ob mice. We found no difference in body weight or body composition between mice treated with APCs and untreated mice. A 4 week oral administration of APCs containing water [0.5% (w/v)] ameliorated glucose tolerance, insulin resistance, and hepatic gluconeogenesis in ob/ob mice. APCs also suppressed the increase in the level of the pancreatic β-cell. Insulin-stimulated Akt phosphorylation was significantly enhanced; pro-inflammatory cytokine expression levels were significantly decreased, and c-Jun N-terminal kinase phosphorylation was downregulated in the liver of those mice treated with APCs. In conclusion, APCs ameliorate insulin resistance by improving hepatic insulin signaling through suppression of hepatic inflammation in ob/ob mice, which may be a mechanism with possible beneficial health effects of APCs in disturbed glucose metabolism.
German Radar Observation Shuttle Experiment (ROSE)
NASA Technical Reports Server (NTRS)
Sleber, A. J.; Hartl, P.; Haydn, R.; Hildebrandt, G.; Konecny, G.; Muehlfeld, R.
1984-01-01
The success of radar sensors in several different application areas of interest depends on the knowledge of the backscatter of radar waves from the targets of interest, the variance of these interaction mechanisms with respect to changing measurement parameters, and the determination of the influence of he measuring systems on the results. The incidence-angle dependency of the radar cross section of different natural targets is derived. Problems involved by the combination of data gained with different sensors, e.g., MSS-, TM-, SPOTand SAR-images are analyzed. Radar cross-section values gained with ground-based radar spectrometers and spaceborne radar imaging, and non-imaging scatterometers and spaceborne radar images from the same areal target are correlated. The penetration of L-band radar waves into vegetated and nonvegetated surfaces is analyzed.
Akaishi, Tetsuya; Takahashi, Toshiyuki; Nakashima, Ichiro
2018-04-15
To elucidate the etiology and clinical significance of periventricular lesions (PVLs) and oligoclonal bands (OB) in multiple sclerosis (MS). We enrolled a total of 97 MS patients (67 OB-positive and 30 OB-negative) who were negative both for anti-aquaporin-4 (AQP4) autoantibody and anti-myelin oligodendrocyte glycoprotein (MOG) antibody. In these patients, comprehensive data including clinical, laboratory, and MRI were collected to investigate the significance of OB and PVLs. Within the MS patients, OB-positivity and the number of PVLs were associated each other. Both of OB-positivity and PVLs did not affect clinical severity or relapse rate, though the patients with ≥3 PVLs showed faster cerebral atrophy than those with <3 PVLs. The patients with OB or ≥3 PVLs showed lower values of quotient of albumin (QAlb), a biomarker of blood-brain barrier (BBB)-permeability and possibly reflects the ongoing disease activity, and lower protein levels in cerebrospinal fluid (CSF). The causal relationship between the decreased QAlb and OB or ≥3 PVLs was not determined. OB and PVLs were associated each other, but they did not affect the clinical course or increased the BBB-permeability within MS patients. Copyright © 2018 Elsevier B.V. All rights reserved.
NASA Technical Reports Server (NTRS)
Kacenelenbogen, M.; Vaughan, M. A.; Redemann, J.; Hoff, R. M.; Rogers, R. R.; Ferrare, R. A.; Russell, P. B.; Hostetler, C. A.; Hair, J. W.; Holben, B. N.
2011-01-01
The Cloud Aerosol LIdar with Orthogonal Polarization (CALIOP), on board the CALIPSO platform, has measured profiles of total attenuated backscatter coefficient (level 1 products) since June 2006. CALIOP s level 2 products, such as the aerosol backscatter and extinction coefficient profiles, are retrieved using a complex succession of automated algorithms. The goal of this study is to help identify potential shortcomings in the CALIOP version 2 level 2 aerosol extinction product and to illustrate some of the motivation for the changes that have been introduced in the next version of CALIOP data (version 3, released in June 2010). To help illustrate the potential factors contributing to the uncertainty of the CALIOP aerosol extinction retrieval, we focus on a one-day, multi-instrument, multiplatform comparison study during the CALIPSO and Twilight Zone (CATZ) validation campaign on 4 August 2007. On that day, we observe a consistency in the Aerosol Optical Depth (AOD) values recorded by four different instruments (i.e. spaceborne MODerate Imaging Spectroradiometer, MODIS: 0.67 and POLarization and Directionality of Earth s Reflectances, POLDER: 0.58, airborne High Spectral Resolution Lidar, HSRL: 0.52 and ground-based AErosol RObotic NETwork, AERONET: 0.48 to 0.73) while CALIOP AOD is a factor of two lower (0.32 at 532 nm). This case study illustrates the following potential sources of uncertainty in the CALIOP AOD: (i) CALIOP s low signal-to-noise ratio (SNR) leading to the misclassification and/or lack of aerosol layer identification, especially close to the Earth s surface; (ii) the cloud contamination of CALIOP version 2 aerosol backscatter and extinction profiles; (iii) potentially erroneous assumptions of the aerosol extinction-to-backscatter ratio (Sa) used in CALIOP s extinction retrievals; and (iv) calibration coefficient biases in the CALIOP daytime attenuated backscatter coefficient profiles. The use of version 3 CALIOP extinction retrieval for our case study seems to partially fix factor (i) although the aerosol retrieved by CALIOP is still somewhat lower than the profile measured by HSRL; the cloud contamination (ii) appears to be corrected; no particular change is apparent in the observation-based CALIOP Sa value (iii). Our case study also showed very little difference in version 2 and version 3 CALIOP attenuated backscatter coefficient profiles, illustrating a minor change in the calibration scheme (iv).
Huang, Chien-Yu; Chung, Chun-I; Lin, Yao-Cheng; Hsing, Yue-Ie Caroline; Huang, Anthony H.C.
2009-01-01
Searches of sequenced genomes of diverse organisms revealed that the moss Physcomitrella patens is the most primitive organism possessing oleosin genes. Microscopy examination of Physcomitrella revealed that oil bodies (OBs) were abundant in the photosynthetic vegetative gametophyte and the reproductive spore. Chromatography illustrated the neutral lipids in OBs isolated from the gametophyte to be largely steryl esters and triacylglycerols, and SDS-PAGE showed the major proteins to be oleosins. Reverse transcription-PCR revealed the expression of all three oleosin genes to be tissue specific. This tissue specificity was greatly altered via alternative splicing, a control mechanism of oleosin gene expression unknown in higher plants. During the production of sex organs at the tips of gametophyte branches, the number of OBs in the top gametophyte tissue decreased concomitant with increases in the number of peroxisomes and level of transcripts encoding the glyoxylate cycle enzymes; thus, the OBs are food reserves for gluconeogenesis. In spores during germination, peroxisomes adjacent to OBs, along with transcripts encoding the glyoxylate cycle enzymes, appeared; thus, the spore OBs are food reserves for gluconeogenesis and equivalent to seed OBs. The one-cell-layer gametophyte could be observed easily with confocal microscopy for the subcellular OBs and other structures. Transient expression of various gene constructs transformed into gametophyte cells revealed that all OBs were linked to the endoplasmic reticulum (ER), that oleosins were synthesized in extended regions of the ER, and that two different oleosins were colocated in all OBs. PMID:19420327
Macronutrients and micronutrients of soybean oil bodies extracted at different pH.
Chen, Yeming; Cao, Yanyun; Zhao, Luping; Kong, Xiangzhen; Hua, Yufei
2014-07-01
In this study, the macronutrients and micronutrients of pH 6.8, 8.0, 9.5, and 11.0 extracted soybean oil bodies (OBs) were examined, revealing that soybean OBs might be used as a natural carrier for bioactive components (unsaturated fatty acids, phospholipid, tocopherol, and phytosterol). pH 6.8 extracted OBs (dry basis) contained 85.88% neutral lipid, 8.18% protein, and 5.85% polar lipid (mainly phospholipid) by gravimetric analysis. The percentage of neutral lipid was increased, while those of protein and polar lipid were decreased with increasing pH. Tocopherol (about 75 mg/100 g neutral lipid of OBs) was not affected, while phytosterol was decreased (136 to 110 mg/100 g neutral lipid of OBs) with increasing pH. The detectable total monosaccaride (galactosamine, glucosamine, and glucose) content of extracted OBs was low and also decreased (35.80 to 6.13 mg/100 g neutral lipid of OBs) with increasing pH. The protein of extracted OBs had higher percentage of essential amino acids than soybean protein isolate with tryptophan and methionine as limited amino acids. The fatty acid composition of extracted OBs was rich in linoleic acid (about 59%), oleic acid (about 20%), and α-linolenic acid (about 7%). Oil bodies (OBs) from soybean and other plant seeds are greatly examined owing to their potential utilizations in food ingredients. The determination of its macronutrients and micronutrients would be very meaningful for its efficient utilization in the future. © 2014 Institute of Food Technologists®
Sirtuin-2 Regulates Sepsis Inflammation in ob/ob Mice
Wang, Xianfeng; Buechler, Nancy L.; Martin, Ayana; Wells, Jonathan; Yoza, Barbara; McCall, Charles E.; Vachharajani, Vidula
2016-01-01
Objective Obesity increases morbidity and resource utilization in sepsis patients. Sepsis transitions from early/hyper-inflammatory to late/hypo-inflammatory phase. Majority of sepsis-mortality occurs during the late sepsis; no therapies exist to treat late sepsis. In lean mice, we have shown that sirtuins (SIRTs) modulate this transition. Here, we investigated the role of sirtuins, especially the adipose-tissue abundant SIRT-2 on transition from early to late sepsis in obese with sepsis. Methods Sepsis was induced using cecal ligation and puncture (CLP) in ob/ob mice. We measured microvascular inflammation in response to lipopolysaccharide/normal saline re-stimulation as a “second-hit” (marker of immune function) at different time points to track phases of sepsis in ob/ob mice. We determined SIRT-2 expression during different phases of sepsis. We studied the effect of SIRT-2 inhibition during the hypo-inflammatory phase on immune function and 7-day survival. We used a RAW264.7 (RAW) cell model of sepsis for mechanistic studies. We confirmed key findings in diet induced obese (DIO) mice with sepsis. Results We observed that the ob/ob-septic mice showed an enhanced early inflammation and a persistent and prolonged hypo-inflammatory phase when compared to WT mice. Unlike WT mice that showed increased SIRT1 expression, we found that SIRT2 levels were increased in ob/ob mice during hypo-inflammation. SIRT-2 inhibition in ob/ob mice during the hypo-inflammatory phase of sepsis reversed the repressed microvascular inflammation in vivo via activation of endothelial cells and circulating leukocytes and significantly improved survival. We confirmed the key finding of the role of SIRT2 during hypo-inflammatory phase of sepsis in this project in DIO-sepsis mice. Mechanistically, in the sepsis cell model, SIRT-2 expression modulated inflammatory response by deacetylation of NFκBp65. Conclusion SIRT-2 regulates microvascular inflammation in obese mice with sepsis and may provide a novel treatment target for obesity with sepsis. PMID:27500833
Moriguchi, E H; Moriguchi, Y; Yamori, Y
2004-12-01
1. Japanese immigrants from Okinawa living in Brazil have a higher mortality from cardiovascular diseases and have their mean life expectancy shortened compared with their counterparts living in Japan. 2. A cross-sectional study comparing Okinawans living in Okinawa (OO) and Okinawan immigrants living in Brazil (OB) was designed to characterize the dietary factors that could interfere with the profile of cardiovascular risk factors and with this reduction on the life expectancy when Okinawans emigrate to Brazil. 3. In total, 234 OO and 160 OB (aged 45-59 years) were recruited to the present study to undergo medical and dietary history, blood pressure measurement, electrocardiograph (ECG), blood tests and 24 h food/urine collection. 4. In the present study, OO subjects presented with 37% less obesity and 50% less systemic hypertension than OB. The OB subjects used threefold more antihypertensive medication than OO. Meat intake was 34% higher in OB than OO, whereas fish intake was sevenfold higher in OO than OB. Serum potassium levels were 10% higher in OO than OB. Urinary taurine (an index of seafood intake) was 43% higher in OO than OB. Urinary isoflavones (an index of the intake of soy products) were significantly lower in OB than in OO. Of acid (20:5) and docosahexaenoic acid (22:6) were two- and threefold higher in OO than OB, respectively. 5. The rate of ischaemic ECG changes in OO subjects was only 50% of that of OB subjects. 6. There were no differences in the smoking rate between OO and OB subjects. 7. The results of the present study suggest that coronary risk factors and cardiovascular health are not only regulated by genetic factors, but that the impact of lifestyle (mainly diet) can be large enough to modulate the expression of genes.
Holsen, L M; Savage, C R; Martin, L E; Bruce, A S; Lepping, R J; Ko, E; Brooks, W M; Butler, M G; Zarcone, J R; Goldstein, J M
2012-05-01
The majority of research on obesity (OB) has focused primarily on clinical features (eating behavior, adiposity measures) or peripheral appetite-regulatory peptides (leptin, ghrelin). However, recent functional neuroimaging studies have demonstrated that some reward circuitry regions that are associated with appetite-regulatory hormones are also involved in the development and maintenance of OB. Prader-Willi syndrome (PWS), characterized by hyperphagia and hyperghrelinemia reflecting multi-system dysfunction in inhibitory and satiety mechanisms, serves as an extreme model of genetic OB. Simple (non-PWS) OB represents an OB-control state. This study investigated subcortical food motivation circuitry and prefrontal inhibitory circuitry functioning in response to food stimuli before and after eating in individuals with PWS compared with OB. We hypothesized that groups would differ in limbic regions (that is, hypothalamus, amygdala) and prefrontal regions associated with cognitive control (that is, dorsolateral prefrontal cortex (DLPFC), orbitofrontal cortex (OFC) after eating. A total of 14 individuals with PWS, 14 BMI- and age-matched individuals with OB, and 15 age-matched healthy-weight controls viewed food and non-food images while undergoing functional MRI before (pre-meal) and after (post-meal) eating. Using SPM8, group contrasts were tested for hypothesized regions: hypothalamus, nucleus accumbens (NAc), amygdala, hippocampus, OFC, medial PFC and DLPFC. Compared with OB and HWC, PWS demonstrated higher activity in reward/limbic regions (NAc, amygdala) and lower activity in the hypothalamus and hippocampus in response to food (vs non-food) images pre-meal. Post meal, PWS exhibited higher subcortical activation (hypothalamus, amygdala, hippocampus) compared with OB and HWC. OB showed significantly higher activity versus PWS and HWC in cortical regions (DLPFC, OFC) associated with inhibitory control. In PWS, compared with OB per se, results suggest hyperactivations in subcortical reward circuitry and hypoactivations in cortical inhibitory regions after eating, which provides evidence of neural substrates associated with variable abnormal food motivation phenotypes in PWS and simple OB.
Sommer, Christine; Lee, Sindre; Gulseth, Hanne Løvdal; Jensen, Jørgen; Drevon, Christian A; Birkeland, Kåre Inge
2018-03-01
Plasma soluble leptin receptor (sOb-R) seems protective of gestational and type 2 diabetes in observational studies, but the mechanisms are unknown. sOb-R is formed by ectodomain shedding of membrane-bound leptin receptors (Ob-Rs), but its associations with messenger RNA (mRNA) expression are scarcely explored. To explore associations between plasma levels of sOb-R and (1) insulin sensitivity, (2) mRNA pathways in adipose tissue and skeletal muscle, and (3) mRNA of candidate genes for sOb-R generation in adipose tissue and skeletal muscle. The MyoGlu study included 26 sedentary, middle-aged men who underwent a 12-week intensive exercise intervention. We measured plasma sOb-R with enzyme-linked immunosorbent assay, insulin sensitivity with a hyperinsulinemic euglycemic clamp, and mRNA in skeletal muscle and adipose tissue with high-throughput sequencing. Baseline plasma sOb-R was strongly associated with baseline glucose infusion rate (GIR) [β (95% confidence interval), 1.19 (0.57 to 1.82) mg/kg/min, P = 0.0006] and GIR improvement after the exercise intervention [0.58 (0.03 to 1.12) mg/kg/min, P = 0.039], also independently of covariates, including plasma leptin. In pathway analyses, high plasma sOb-R correlated with upregulation of metabolic pathways and downregulation of inflammatory pathways in both adipose tissue and skeletal muscle. In skeletal muscle, mRNA of LEPROT and LEPROTL1 (involved in Ob-R cell surface expression) and ADAM10 and ADAM17 (involved sOb-R-shedding) increased after the exercise intervention. Higher plasma sOb-R was associated with improved GIR, upregulation of metabolic pathways, and downregulation of inflammatory pathways, which may be possible mechanisms for the seemingly protective effect of plasma sOb-R on subsequent risk of gestational and type 2 diabetes found in observational studies.
Gender Dimorphism in Skeletal Muscle Leptin Receptors, Serum Leptin and Insulin Sensitivity
Guerra, Borja; Fuentes, Teresa; Delgado-Guerra, Safira; Guadalupe-Grau, Amelia; Olmedillas, Hugo; Santana, Alfredo; Ponce-Gonzalez, Jesus Gustavo; Dorado, Cecilia; Calbet, José A. L.
2008-01-01
To determine if there is a gender dimorphism in the expression of leptin receptors (OB-R170, OB-R128 and OB-R98) and the protein suppressor of cytokine signaling 3 (SOCS3) in human skeletal muscle, the protein expression of OB-R, perilipin A, SOCS3 and alpha-tubulin was assessed by Western blot in muscle biopsies obtained from the m. vastus lateralis in thirty-four men (age = 27.1±6.8 yr) and thirty-three women (age = 26.7±6.7 yr). Basal serum insulin concentration and HOMA were similar in both genders. Serum leptin concentration was 3.4 times higher in women compared to men (P<0.05) and this difference remained significant after accounting for the differences in percentage of body fat or soluble leptin receptor. OB-R protein was 41% (OB-R170, P<0.05) and 163% (OB-R128, P<0.05) greater in women than men. There was no relationship between OB-R expression and the serum concentrations of leptin or 17β-estradiol. In men, muscle OB-R128 protein was inversely related to serum free testosterone. In women, OB-R98 and OB-R128 were inversely related to total serum testosterone concentration, and OB-R128 to serum free testosterone concentration. SOCS3 protein expression was similar in men and women and was not related to OB-R. In women, there was an inverse relationship between the logarithm of free testosterone and SCOS3 protein content in skeletal muscle (r = −0.46, P<0.05). In summary, there is a gender dimorphism in skeletal muscle leptin receptors expression, which can be partly explained by the influence of testosterone. SOCS3 expression in skeletal muscle is not up-regulated in women, despite very high serum leptin concentrations compared to men. The circulating form of the leptin receptor can not be used as a surrogate measure of the amount of leptin receptors expressed in skeletal muscles. PMID:18941624
SPM response to tide and river flow in the hyper-turbid Ems River
NASA Astrophysics Data System (ADS)
Winterwerp, Johan C.; Vroom, Julia; Wang, Zheng-B.; Krebs, Martin; Hendriks, Erik C. M.; van Maren, Dirk S.; Schrottke, Kerstin; Borgsmüller, Christine; Schöl, Andreas
2017-05-01
In this paper, we analyse the behaviour of fine sediments in the hyper-turbid Lower Ems River, with focus on the river's upper reaches, a stretch of about 25 km up-estuary of Terborg. Our analysis is based on long records of suspended particulate matter (SPM) from optical backscatter (OBS) measurements close to the bed at seven stations along the river, records of salinity and water level measurements at these stations, acoustic measurements on the vertical mud structure just up-estuary of Terborg and oxygen profiles in the lower 3 m of the water column close to Leerort and Terborg. Further, we use cross-sectionally averaged velocities computed with a calibrated numerical model. Distinction is made between four timescales, i.e. the semi-diurnal tidal timescale, the spring-neap tidal timescale, a timescale around an isolated peak in river flow (i.e. about 3 weeks) and a seasonal timescale. The data suggest that a pool of fluid/soft mud is present in these upper reaches, from up-estuary of Papenburg to a bit down-estuary of Terborg. Between Terborg and Gandersum, SPM values drop rapidly but remain high at a few gram per litre. The pool of fluid/soft mud is entrained/mobilized at the onset of flood, yielding SPM values of many tens gram per litre. This suspension is transported up-estuary with the flood. Around high water slack, part of the suspension settles, being remixed during ebb, while migrating down-estuary, but likely not much further than Terborg. Around low water slack, a large fraction of the sediment settles, reforming the pool of fluid mud. The rapid entrainment from the fluid mud layer after low water slack is only possible when the peak flood velocity exceeds a critical value of around 1 m/s, i.e. when the stratified water column seems to become internally supercritical. If the peak flood velocity does not reach this critical value, f.i. during neap tide, fluid mud is not entrained up to the OBS sensors. Thus, it is not classical tidal asymmetry, but the peak flood velocity itself which governs the hyper-turbid state in the Lower Ems River. The crucial role of river flow and river floods is in reducing these peak flood velocities. During elongated periods of high river flow, in e.g. wintertime, SPM concentrations reduce, and the soft mud deposits consolidate and possibly become locally armoured as well by sand washed in from the river. We have no observations that sediments are washed out of the hyper-turbid zone. Down-estuary of Terborg, where SPM values do not reach hyper-turbid conditions, the SPM dynamics are governed by classical tidal asymmetry and estuarine circulation. Hence, nowhere in the river, sediments are flushed from the upper reaches of the river into the Ems-Dollard estuary during high river flow events. However, exchange of sediment between river and estuary should occur because of tide-induced dispersion.
Dense fog on the highway: Visual range monitoring in cars?
NASA Technical Reports Server (NTRS)
Hahn, W.; Krichbaumer, W.; Streicher, J.; Werner, CH.
1992-01-01
This paper reports on the development of a new sensor. Laser range-finders are currently installed in cars and trucks to measure the distance to a proceeding car (LEICA). A modification of such a sensor to measure visibility was made. The problems that had to be solved were: (1) choice of wavelength with relation to the human eye for visibility measurements; (2) dependency of the wavelength on atmospheric turbidity; (3) laser eye-safety; and (4) influence of multiple scattering at visibilities smaller than 200 m. The wavelength used for lidar sensors in the near infrared presents no real problems because the object to be sensed is fog appearing white which means that scattering from fog is wavelength independent. There are however differences in backscatter-to-extinction ratio for different fog and weather situations. The two solutions to these problems are polarization and multiple scattering. As known from airport operations of a laser ceilometer, one can use this multiple scattering contribution to determine the visibility.
Satellite-Sensor Calibration Verification Using the Cloud-Shadow Method
NASA Technical Reports Server (NTRS)
Reinersman, P.; Carder, K. L.; Chen, F. R.
1995-01-01
An atmospheric-correction method which uses cloud-shaded pixels together with pixels in a neighboring region of similar optical properties is described. This cloud-shadow method uses the difference between the total radiance values observed at the sensor for these two regions, thus removing the nearly identical atmospheric radiance contributions to the two signals (e.g. path radiance and Fresnel-reflected skylight). What remains is largely due to solar photons backscattered from beneath the sea to dominate the residual signal. Normalization by the direct solar irradiance reaching the sea surface and correction for some second-order effects provides the remote-sensing reflectance of the ocean at the location of the neighbor region, providing a known 'ground target' spectrum for use in testing the calibration of the sensor. A similar approach may be useful for land targets if horizontal homogeneity of scene reflectance exists about the shadow. Monte Carlo calculations have been used to correct for adjacency effects and to estimate the differences in the skylight reaching the shadowed and neighbor pixels.
An Analysis of the Impact of Outward Bound on Twelve High Schools.
ERIC Educational Resources Information Center
Schulze, Joseph R.
Describing and analyzing the impact of Outward Bound (OB) programs on 12 high schools which reflect OB involvement varying from 1 to 5 years and include urban, suburban, and rural (public, private, boarding, and day) schools, this 1970-71 report is aimed at furthering OB philosophy and method. The report presents OB program: background; evaluation…
Complete Genome Sequence of Escherichia coli Strain M8, Isolated from ob/ob Mice
Siddharth, Jay; Membrez, Mathieu; Chakrabarti, Anirikh; Betrisey, Bertrand; Chou, Chieh Jason
2017-01-01
ABSTRACT Escherichia coli is one of the common inhabitants of the mammalian gastrointestinal track. We isolated a strain from an ob/ob mouse and performed whole-genome sequencing, which yielded a chromosome of ~5.1 Mb and three plasmids of ~160 kb, ~6 kb, and ~4 kb. PMID:28572322
Optical cloud detection from a disposable airborne sensor
NASA Astrophysics Data System (ADS)
Nicoll, Keri; Harrison, R. Giles; Brus, David
2016-04-01
In-situ measurement of cloud droplet microphysical properties is most commonly made from manned aircraft platforms due to the size and weight of the instrumentation, which is both costly and typically limited to sampling only a few clouds. This work describes the development of a small, lightweight (<200g), disposable, optical cloud sensor which is designed for use on routine radiosonde balloon flights and also small unmanned aerial vehicle (UAV) platforms. The sensor employs the backscatter principle, using an ultra-bright LED as the illumination source, with a photodiode detector. Scattering of the LED light by cloud droplets generates a small optical signal which is separated from background light fluctuations using a lock-in technique. The signal to noise obtained permits cloud detection using the scattered LED light, even in daytime. During recent field tests in Pallas, Finland, the retrieved optical sensor signal has been compared with the DMT Cloud and Aerosol Spectrometer (CAS) which measures cloud droplets in the size range from 0.5 to 50 microns. Both sensors were installed at the hill top observatory of Sammaltunturi during a field campaign in October and November 2015, which experienced long periods of immersion inside cloud. Preliminary analysis shows very good agreement between the CAPS and the disposable cloud sensor for cloud droplets >5micron effective diameter. Such data and calibration of the sensor will be discussed here, as will simultaneous balloon launches of the optical cloud sensor through the same cloud layers.
Smsynth: AN Imagery Synthesis System for Soil Moisture Retrieval
NASA Astrophysics Data System (ADS)
Cao, Y.; Xu, L.; Peng, J.
2018-04-01
Soil moisture (SM) is a important variable in various research areas, such as weather and climate forecasting, agriculture, drought and flood monitoring and prediction, and human health. An ongoing challenge in estimating SM via synthetic aperture radar (SAR) is the development of the retrieval SM methods, especially the empirical models needs as training samples a lot of measurements of SM and soil roughness parameters which are very difficult to acquire. As such, it is difficult to develop empirical models using realistic SAR imagery and it is necessary to develop methods to synthesis SAR imagery. To tackle this issue, a SAR imagery synthesis system based on the SM named SMSynth is presented, which can simulate radar signals that are realistic as far as possible to the real SAR imagery. In SMSynth, SAR backscatter coefficients for each soil type are simulated via the Oh model under the Bayesian framework, where the spatial correlation is modeled by the Markov random field (MRF) model. The backscattering coefficients simulated based on the designed soil parameters and sensor parameters are added into the Bayesian framework through the data likelihood where the soil parameters and sensor parameters are set as realistic as possible to the circumstances on the ground and in the validity range of the Oh model. In this way, a complete and coherent Bayesian probabilistic framework is established. Experimental results show that SMSynth is capable of generating realistic SAR images that suit the needs of a large amount of training samples of empirical models.
Combined In-situ and Ground-based Observations of Quasi-periodic Radar Echoes
NASA Astrophysics Data System (ADS)
Pfaff, R.; Kudeki, E.; Larsen, M.; Clemmons, J.; Earle, G.
A series of combined rocket/radar investigation of the electrodynamics and neutral- plasma coupling associated with sporadic-E layers and quasi-periodic backscatter radar echoes has been carried out from launch sites at both Puerto Rico and the Wallops Flight Facility, Virginia (USA) between 1998-2001. The instrumented rock- ets consisted of main and sub-payloads and were launched while strong quasi- periodic VHF echoes were observed simultaneously with the Univ. of Illinois 50 MHz backscatter radar. The rocket apogee was purposely limited so that the payloads would dwell in the sporadic-E region (90-115 km). The main payload included vector DC and AC electric field detectors, a DC magnetometer, an ion mass spectrometer, an ioniza- tion gauge, and spaced-electric field receivers to measure the wavelength and phase velocity of the unstable plasma waves. The sub-payload was instrumented to measure DC and wave electric fields and plasma density. In one case, a separate rocket was launched a few minutes later which released luminous TMA trails to measure the neu- tral wind, its velocity shear, and embedded neutral structures. In this experiment, the payloads successfully pierced a well-defined, 2-3 km thick metallic sporadic-E layer of approximately 10**5 e/cc near 103 km altitude. In-situ DC electric field measure- ments revealed ~5mV/m ambient meridional fields above and below the layer with 1-2 mV/m amplitude, large scale structures superimposed. The wavelengths of these structures were approximately 2-4 km and may be related to the seat of the quasi- periodic echoes. Intense (~5 mV/m), higher frequency (shorter scale) broadband waves were also observed in-situ, both above and below the layer, consistent with the VHF backscatter observations during the time of the launch. Neither the large scale nor short scale plasma waves appeared to be distinctly organized by the sporadic-E den- sity layer. The TMA release showed large amplitude (~ 100 m/s) meridional winds near 102-105 km, with the most intense shears directly below these altitudes, where the short scale electric field fluctuations were most intense. We summarize the ob- servations from the different experiments and discuss them in the context of current theories regarding quasi-periodic echoes.
Current Status Of The NAVSEA Backscatter Absorption Gas Imaging (BAGI) Development Project
NASA Astrophysics Data System (ADS)
Kulp, Thomas J.; Kennedy, Randall B.; Garvis, Darrel G.; McRae, Thomas G.; Stahovec, Joe
1989-07-01
During the last five years, work has been underway at the Lawrence Livermore National Laboratory (LLNL) to develop a method for imaging gas clouds that are normally invisible to the human eye. The effort was initiated to provide an effective means of locating leaks of hazardous vapors. Although conventional point or line-of-sight detectors are well suited to the measurement of gas concentrations, their utility in identifying the origin and direction of travel of gas plumes is limited. To obtain spatial information from sensors that provide only zero- or one-dimensional readings, either sequential readings at many different locations from a single device, or multiplexed simultaneous measurements from a sensor array must be taken. The former approach is time consuming and, therefore, impractical in emergency situations where rapid action is required. The latter is useful only in cases where the probability of a hazardous release is high enough to warrant the prior installation of a sensor network. Either method demands high measuremental precision and sufficient discrimination against both interfering gases and interfering sources of the target gas. Backscatter Absorption Gas Imaging (BAGI) is a new technique that makes gas clouds and their surroundings "visible" in a real-time video image. It is superior to conventional sensors in characterizing the spatial properties of gas clouds because it provides data that are inherently two-dimensional. Less measuremental precision is required by the BAGI technique because it conveys information as contrasts between different areas in an image rather than as absolute concentration values. Furthermore, the pictorial display of this information allows it to be rapidly assimilated by emergency-response teams. The size and orientation of the plume are evident through comparison with familiar objects that also appear in the image. Subtler evaluations can be made as well, such as the distinction between innocous and hazardous sources of the target gas. For example, in using a conventional sensor to search for the source of a gas that is also present at low levels in automobile exhaust, one might be led astray near a highway. Gas imaging allows the searcher to recognize that the cars are producing the gas, and that they are not the objective of the search.
Schaber, G.G.
1999-01-01
Synthetic Aperture Radar (SAR) images acquired over part of the Yuma Desert in southwestern Arizona demonstrate the ability of C-band (5.7-cm wavelength), L-band (24.5 cm), and P-band (68 cm) AIRSAR signals to backscatter from increasingly greater depths reaching several meters in blow sand and sandy alluvium. AIRSAR images obtained within the Barry M. Goldwater Bombing and Gunnery Range near Yuma, Arizona, show a total reversal of C- and P-band backscatter contrast (image tone) for three distinct geologic units. This phenomenon results from an increasingly greater depth of radar imaging with increasing radar wavelength. In the case of sandy- and small pebble-alluvium surfaces mantled by up to several meters of blow sand, backscatter increases directly with SAR wavelength as a result of volume scattering from a calcic soil horizon at shallow depth and by volume scattering from the root mounds of healthy desert vegetation that locally stabilize blow sand. AIRSAR images obtained within the military range are also shown to be useful for detecting metallic military ordnance debris that is located either at the surface or covered by tens of centimeters to several meters of blow sand. The degree of detectability of this ordnance increases with SAR wavelength and is clearly maximized on P-band images that are processed in the cross-polarized mode (HV). This effect is attributed to maximum signal penetration at P-band and the enhanced PHV image contrast between the radar-bright ordnance debris and the radar-dark sandy desert. This article focuses on the interpretation of high resolution AIRSAR images but also Compares these airborne SAR images with those acquired from spacecraft sensors such as ERS-SAR and Space Radar Laboratory (SIR-C/X-SAR).Synthetic Aperture Radar (SAR) images acquired over part of the Yuma Desert in southwestern Arizona demonstrate the ability of C-band (5.7-cm wavelength), L-band (24.5 cm), and P-band (68 cm) AIRSAR signals to backscatter from increasingly greater depths reaching several meters in blow sand and sandy alluvium. AIRSAR images obtained within the Barry M. Goldwater Bombing and Gunnery Range near Yuma, Arizona, show a total reversal of C- and P-band backscatter contrast (image tone) for three distinct geologic units. This phenomenon results from an increasingly greater depth of radar imaging with increasing radar wavelength. In the case of sandy- and small pebble-alluvium surfaces mantled by up to several meters of blow sand, backscatter increases directly with SAR wavelength as a result of volume scattering from a calcic soil horizon at shallow depth and by volume scattering from the root mounds of healthy desert vegetation that locally stabilize blow sand. AIRSAR images obtained within the military range are also shown to be useful for detecting metallic military ordnance debris that is located either at the surface or covered by tens of centimeters to several meters of blow sand. The degree of detectability of this ordnance increases with SAR wavelength and is clearly maximized on P-band images that are processed in the cross-polarized mode (HV). This effect is attributed to maximum signal penetration at P-band and the enhanced PHV image contrast between the radar-bright ordnance debris and the radar-dark sandy desert. This article focuses on the interpretation of high resolution AIRSAR images but also compares these airborne SAR images with those acquired from spacecraft sensors such as ERS-SAR and Space Radar Laboratory (SIR-C/X-SAR).
Latchney, Sarah E; Rivera, Phillip D; Mao, Xiao W; Ferguson, Virginia L; Bateman, Ted A; Stodieck, Louis S; Nelson, Gregory A; Eisch, Amelia J
2014-06-15
Space missions necessitate physiological and psychological adaptations to environmental factors not present on Earth, some of which present significant risks for the central nervous system (CNS) of crewmembers. One CNS region of interest is the adult olfactory bulb (OB), as OB structure and function are sensitive to environmental- and experience-induced regulation. It is currently unknown how the OB is altered by spaceflight. In this study, we evaluated OB volume and neurogenesis in mice shortly after a 13-day flight on Space Shuttle Atlantis [Space Transport System (STS)-135] relative to two groups of control mice maintained on Earth. Mice housed on Earth in animal enclosure modules that mimicked the conditions onboard STS-135 (AEM-Ground mice) had greater OB volume relative to mice maintained in standard housing on Earth (Vivarium mice), particularly in the granule (GCL) and glomerular (GL) cell layers. AEM-Ground mice also had more OB neuroblasts and fewer apoptotic cells relative to Vivarium mice. However, the AEM-induced increase in OB volume and neurogenesis was not seen in STS-135 mice (AEM-Flight mice), suggesting that spaceflight may have negated the positive effects of the AEM. In fact, when OB volume of AEM-Flight mice was considered, there was a greater density of apoptotic cells relative to AEM-Ground mice. Our findings suggest that factors present during spaceflight have opposing effects on OB size and neurogenesis, and provide insight into potential strategies to preserve OB structure and function during future space missions. Copyright © 2014 the American Physiological Society.
Taguchi, Kumiko; Matsumoto, Takayuki; Kamata, Katsuo; Kobayashi, Tsuneo
2012-01-01
In type 2 diabetes, impaired insulin-induced Akt/endothelial nitric oxide synthase (eNOS) signaling may decrease the vascular relaxation response. Previously, we reported that this response was negatively regulated by G protein–coupled receptor kinase 2 (GRK2). In this study, we investigated whether/how in aortas from ob/ob mice (a model of type 2 diabetes) GRK2 and β-arrestin 2 might regulate insulin-induced signaling. Endothelium-dependent relaxation was measured in aortic strips. GRK2, β-arrestin 2, and Akt/eNOS signaling pathway proteins and activities were mainly assayed by Western blotting. In ob/ob (vs. control [Lean]) aortas: 1) insulin-induced relaxation was reduced, and this deficit was prevented by GRK2 inhibitor, anti-GRK2 antibody, and an siRNA specifically targeting GRK2. The Lean aorta relaxation response was reduced to the ob/ob level by pretreatment with an siRNA targeting β-arrestin 2. 2) Insulin-stimulated Akt and eNOS phosphorylations were decreased. 3) GRK2 expression in membranes was elevated, and, upon insulin stimulation, this expression was further increased, but β-arrestin 2 was decreased. In ob/ob aortic membranes under insulin stimulation, the phosphorylations of Akt and eNOS were augmented by GRK2 inhibitor. In mouse aorta, GRK2 may be, upon translocation, a key negative regulator of insulin responsiveness and an important regulator of the β-arrestin 2/Akt/eNOS signaling, which is implicated in diabetic endothelial dysfunction. PMID:22688330
Taguchi, Kumiko; Matsumoto, Takayuki; Kamata, Katsuo; Kobayashi, Tsuneo
2012-08-01
In type 2 diabetes, impaired insulin-induced Akt/endothelial nitric oxide synthase (eNOS) signaling may decrease the vascular relaxation response. Previously, we reported that this response was negatively regulated by G protein-coupled receptor kinase 2 (GRK2). In this study, we investigated whether/how in aortas from ob/ob mice (a model of type 2 diabetes) GRK2 and β-arrestin 2 might regulate insulin-induced signaling. Endothelium-dependent relaxation was measured in aortic strips. GRK2, β-arrestin 2, and Akt/eNOS signaling pathway proteins and activities were mainly assayed by Western blotting. In ob/ob (vs. control [Lean]) aortas: 1) insulin-induced relaxation was reduced, and this deficit was prevented by GRK2 inhibitor, anti-GRK2 antibody, and an siRNA specifically targeting GRK2. The Lean aorta relaxation response was reduced to the ob/ob level by pretreatment with an siRNA targeting β-arrestin 2. 2) Insulin-stimulated Akt and eNOS phosphorylations were decreased. 3) GRK2 expression in membranes was elevated, and, upon insulin stimulation, this expression was further increased, but β-arrestin 2 was decreased. In ob/ob aortic membranes under insulin stimulation, the phosphorylations of Akt and eNOS were augmented by GRK2 inhibitor. In mouse aorta, GRK2 may be, upon translocation, a key negative regulator of insulin responsiveness and an important regulator of the β-arrestin 2/Akt/eNOS signaling, which is implicated in diabetic endothelial dysfunction.
Bernal, Alexandra; Simón, Oihane; Williams, Trevor; Muñoz, Delia; Caballero, Primitivo
2018-01-03
A Chrysodeixis chalcites nucleopolyhedrovirus from the Canary Islands (ChchNPV-TF1) has proved to be effective for control of Chrysodeixis chalcites on banana crops. Commercialization of this virus as a bioinsecticide requires an efficient production system. The sixth instar (L 6 ) was the most suitable for virus production, producing 1.80 × 10 11 occlusion bodies (OB)/larva and showed a lower prevalence of cannibalism (5.4%) than fourth (L 4 ) or fifth (L 5 ) instars. Inoculation of L 6 at 24 h post molting produced six times more OB (5.72 × 10 11 OB/larva) than recently molted L 6 larvae (1.00 × 10 11 OB/larva). No significant differences were recorded in mean time to death (165-175 h) or OB production per larva (3.75 × 10 11 to 5.97 × 10 11 ) or per mg larval weight (1.30 × 10 11 to 2.11 × 10 9 ), in larvae inoculated with a range of inoculum concentrations (LC 50 -LC 90 ). Groups of infected L 6 larvae reared at a density of 150 larvae/container produced a greater total number of OBs (8.07 × 10 13 OB/container) than lower densities (25, 50 and 100 OB/container), and a similar number to containers with 200 inoculated larvae (8.43 × 10 13 OB/container). The processes described here allow efficient production of sufficient OBs to treat ∼ 40 ha of banana crops using the insects from a single container. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.
Autophagy in osteoblasts is involved in mineralization and bone homeostasis
Nollet, Marie; Santucci-Darmanin, Sabine; Breuil, Véronique; Al-Sahlanee, Rasha; Cros, Chantal; Topi, Majlinda; Momier, David; Samson, Michel; Pagnotta, Sophie; Cailleteau, Laurence; Battaglia, Séverine; Farlay, Delphine; Dacquin, Romain; Barois, Nicolas; Jurdic, Pierre; Boivin, Georges; Heymann, Dominique; Lafont, Frank; Lu, Shi Shou; Dempster, David W; Carle, Georges F; Pierrefite-Carle, Valérie
2014-01-01
Bone remodeling is a tightly controlled mechanism in which osteoblasts (OB), the cells responsible for bone formation, osteoclasts (OC), the cells specialized for bone resorption, and osteocytes, the multifunctional mechanosensing cells embedded in the bone matrix, are the main actors. Increased oxidative stress in OB, the cells producing and mineralizing bone matrix, has been associated with osteoporosis development but the role of autophagy in OB has not yet been addressed. This is the goal of the present study. We first show that the autophagic process is induced in OB during mineralization. Then, using knockdown of autophagy-essential genes and OB-specific autophagy-deficient mice, we demonstrate that autophagy deficiency reduces mineralization capacity. Moreover, our data suggest that autophagic vacuoles could be used as vehicles in OB to secrete apatite crystals. In addition, autophagy-deficient OB exhibit increased oxidative stress and secretion of the receptor activator of NFKB1 (TNFSF11/RANKL), favoring generation of OC, the cells specialized in bone resorption. In vivo, we observed a 50% reduction in trabecular bone mass in OB-specific autophagy-deficient mice. Taken together, our results show for the first time that autophagy in OB is involved both in the mineralization process and in bone homeostasis. These findings are of importance for mineralized tissues which extend from corals to vertebrates and uncover new therapeutic targets for calcified tissue-related metabolic pathologies. PMID:25484092
Kulkarni, Supriya R.; Xu, Jialin; Donepudi, Ajay C.; Wei, Wei
2014-01-01
Purpose Fatty liver alters liver transporter expression. Caloric restriction (CR), the recommended therapy to reverse fatty liver, increases Sirtuin1 deacetylase activity in liver. This study evaluated whether CR and CR mimetics reversed obesity-induced transporter expression in liver and hepatocytes. Methods mRNA and protein expression was determined in adult lean (lean) and leptin-deficient obese (OB) mice fed ad libitum or placed on 40% (kCal) reduced diet. Hepatocytes were isolated from lean and OB mice, treated with AMP Kinase activators, and gene expression was determined. Results CR decreased Oatp1a1, Oatp1b2, and Abcb11 mRNA expression in lean, but not OB mice. CR increased Abcc2 mRNA OB livers, whereas protein expression increased in both genotypes. CR increased Abcc3 protein expression increased in OB livers. CR did not alter Abcc1, 4 and 5 mRNA expression in lean mice but decreased expression in livers of OB mice. CR increased Abcc4 protein in lean, but not OB mice. Conclusions CR restriction reversed the expression of some, but not all transporters in livers of OB mice. Overall, these data indicate a potential for CR to restore some hepatic transporter changes in OB mice, but suggest a functional leptin axis is needed for reversal of expression for some transporters. PMID:23949303
Tobe, Seiichi; Kamezaki, Hiroki; Watanabe, Toshiyuki; Takaoka, Hiromitsu; Sakaguchi, Masahiro
2010-01-01
House dust mites (Dermatophagoides farinae) in bedding and clothes are a major allergen. However, house dust mites cannot be killed by general washing conditions under 50 degrees C. Therefore, low-temperature washing conditions must be improved to eliminate house dust mites. Sodium alkyl acyloxybenzene sulfonate (OBS) is a bleach activator that is used to intensify the bleaching effects of some laundry products. The purpose of this study was to evaluate the effect of OBS on the elimination of house dust mites in low-temperature washing conditions. D. farinae was soaked in solutions containing different types of OBS for various durations and at various temperatures. The miticidal effects of the various washing conditions were also evaluated for D. farinae. Then sodium lauroyloxybenzene sulfonate (OBS-12) produced the highest D. farinae mortality rate among the OBS solutions that were examined and had a stronger miticidal effect than available chlorine under general washing conditions. OBS exhibited miticidal effects under general washing conditions at low temperatures. Since OBS is already used as an additive in some laundry products to increase the bleaching activity, OBS can be easily used to kill house dust mites under general washing conditions.
Gu, Jianwei; Chao, Hongbo; Wang, Hao; Li, Yonghong; Li, Dianrong; Xiang, Jun; Gan, Jianping; Lu, Guangyuan; Zhang, Xuekun; Long, Yan; Li, Maoteng
2017-01-01
Oil bodies (OBs) are relatively simple but very important organelles comprising a matrix of triacylglycerol (TAG) surrounded by a phospholipid monolayer embedded and covered with unique proteins. The OB structure in Brassica napus with different oil content and the relationship between the oil content and the OB structure needs to be better understood. In this paper, the characteristics of OBs in the embryo of a series of B. napus materials with different oil content ranging from 34% to over 60% were studied. The results indicated that the OB size was significantly positively correlated with the oil content but was significantly negatively correlated with the glucosinolates and the protein content. Many genes associated with TAG synthesis, OB-membrane proteins, and the cell progress regulatory pathway were identified in the confidence interval of co-located QTLs for oil content, fatty acid (FA) compositions, and protein content. Our results suggested that the morphology of OBs might be directly controlled by the genes associated with OB-membrane proteins and indirectly controlled by the genes associated with TAG synthesis and cell progress regulatory pathway. PMID:28111582
Gu, Jianwei; Chao, Hongbo; Wang, Hao; Li, Yonghong; Li, Dianrong; Xiang, Jun; Gan, Jianping; Lu, Guangyuan; Zhang, Xuekun; Long, Yan; Li, Maoteng
2016-01-01
Oil bodies (OBs) are relatively simple but very important organelles comprising a matrix of triacylglycerol (TAG) surrounded by a phospholipid monolayer embedded and covered with unique proteins. The OB structure in Brassica napus with different oil content and the relationship between the oil content and the OB structure needs to be better understood. In this paper, the characteristics of OBs in the embryo of a series of B. napus materials with different oil content ranging from 34% to over 60% were studied. The results indicated that the OB size was significantly positively correlated with the oil content but was significantly negatively correlated with the glucosinolates and the protein content. Many genes associated with TAG synthesis, OB-membrane proteins, and the cell progress regulatory pathway were identified in the confidence interval of co-located QTLs for oil content, fatty acid (FA) compositions, and protein content. Our results suggested that the morphology of OBs might be directly controlled by the genes associated with OB-membrane proteins and indirectly controlled by the genes associated with TAG synthesis and cell progress regulatory pathway.
Oil body proteins sequentially accumulate throughout seed development in Brassica napus.
Jolivet, Pascale; Boulard, Céline; Bellamy, Annick; Valot, Benoît; d'Andréa, Sabine; Zivy, Michel; Nesi, Nathalie; Chardot, Thierry
2011-11-15
Despite the importance of seed oil bodies (OBs) as enclosed compartments for oil storage, little is known about lipid and protein accumulation in OBs during seed formation. OBs from rapeseed (Brassica napus) consist of a triacylglycerol (TAG) core surrounded by a phospholipid monolayer embedded with integral proteins which confer high stability to OBs in the mature dry seed. In the present study, we investigated lipid and protein accumulation patterns throughout seed development (from 5 to 65 days after pollination [DAP]) both in the whole seed and in purified OBs. Deposition of the major proteins (oleosins, caleosins and steroleosins) into OBs was assessed through (i) gene expression pattern, (ii) proteomics analysis, and (iii) protein immunodetection. For the first time, a sequential deposition of integral OB proteins was established. Accumulation of oleosins and caleosins was observed starting from early stages of seed development (12-17 DAP), while steroleosins accumulated later (~25 DAP) onwards. Copyright © 2011 Elsevier GmbH. All rights reserved.
The Puppis region and the last crusade for faint OB stars
NASA Astrophysics Data System (ADS)
Orsatti, Ana M.
1992-08-01
UBV photoelectric and photographic measurements of OB stars from a list of 397 OB stars and 5 early-type supergiants and from the Luminous Stars Survey are presented. The galactic distribution of the OB stars in the region shows concentrations around the open clusters Ruprecht 44 and Ruprecht 55, and the presence of an important grouping of young stars located far below the plane. The distribution in latitude shows that young stars in the region are not restricted to a thin sheet around the plane but are spread over negative latitudes reaching at least b = -5 deg. In longitude, the OB distribution exhibits a concentration of Ob stars in the interval 244-251 deg; this is argued to be due to the presence of the local arm extension.
What is an Obstetrics/Gynecology Hospitalist?
McCue, Brigid
2015-09-01
The obstetrics/gynecology (OB/GYN) hospitalist is the latest subspecialist to evolve from obstetrics and gynecology. Starting in 2002, academic leaders recognized the impact of such coalescing forces as the pressure to reduce maternal morbidity and mortality, stagnant reimbursements and the increasing cost of private practice, the decrease in applications for OB/GYN residencies, and the demand among practicing OB/GYNs for work/life balance. Initially coined laborist, the concept of the OB/GYN hospitalist emerged. Thinking of becoming an OB/GYN hospitalist? Here is what you need to know. Copyright © 2015 Elsevier Inc. All rights reserved.
Du, Shu-Fang; Yu, Qing; Chuan, Kai; Ye, Chang-Lin; He, Ze-Jia; Liu, Shu-Juan; Zhu, Xiao-Yan; Liu, Yu-Jian
2017-10-01
Exercise training is advocated for treating chronic inflammation and obesity-related metabolic syndromes. Glucocorticoids (GCs), the anti-inflammatory hormones, are synthesized or metabolized in extra-adrenal organs. This study aims to examine whether exercise training affects obesity-associated pulmonary inflammation by regulating local GC synthesis or metabolism. We found that sedentary obese ( ob/ob ) mice exhibited increased levels of interleukin (IL)-1β, IL-18, monocyte chemotactic protein (MCP)-1, and leukocyte infiltration in lung tissues compared with lean mice, which was alleviated by 6 wk of exercise training. Pulmonary corticosterone levels were decreased in ob/ob mice. Exercise training increased pulmonary corticosterone levels in both lean and ob/ob mice. Pulmonary corticosterone levels were negatively correlated with IL-1β, IL-18, and MCP-1. Immunohistochemical staining of the adult mouse lung sections revealed positive immunoreactivities for the steroidogenic acute regulatory protein, the cholesterol side-chain cleavage enzyme (CYP11A1), the steroid 21-hydroxylase (CYP21), 3β-hydroxysteroid dehydrogenase (3β-HSD), and type 1 and type 2 11β-hydroxysteroid dehydrogenase (11β-HSD) but not for 11β-hydroxylase (CYP11B1). Exercise training significantly increased pulmonary 11β-HSD1 expression in both lean and ob/ob mice. In contrast, exercise training per se had no effect on pulmonary 11β-HSD2 expression, although pulmonary 11β-HSD2 levels in ob/ob mice were significantly higher than in lean mice. RU486, a glucocorticoid receptor antagonist, blocked the anti-inflammatory effects of exercise training in lung tissues of obese mice and increased inflammatory cytokines in lean exercised mice. These findings indicate that exercise training increases pulmonary expression of 11β-HSD1, thus contributing to local GC activation and suppression of pulmonary inflammation in obese mice. NEW & NOTEWORTHY Treadmill training leads to a significant increase in pulmonary corticosterone levels in ob/ob mice, which is in parallel with the favorable effects of exercise on obesity-associated pulmonary inflammation. Exercise training increases pulmonary 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) expression but has no significant effect on 11β-HSD2 expression in both lean and ob/ob mice. These findings indicate that exercise training increases pulmonary expression of 11β-HSD1, thus contributing to local glucocorticoid activation and suppression of pulmonary inflammation in obese mice. Copyright © 2017 the American Physiological Society.
NASA Astrophysics Data System (ADS)
Spencer, G.; Llewellyn-Jones, D.
In the summer of 2002 the Meteosat Second Generation (MSG) satellite is due to be launched. On board the MSG satellite is the Geostationary Earth Radiation Budget (GERB) sensor. This is a new radiometer that will be able to observe and measure the outgoing longwave radiation from the top of the atmosphere for the whole ob- served Earth disc, due to its unique position in geostationary orbit. Every 15 minutes the GERB sensor will make a full Earth disc observation, centred on the Greenwich meridian. Thus, the GERB sensor will provide unprecedented coupled temporal and spatial resolution of the outgoing longwave radiation (4.0 to 30.0 microns), by first measuring the broadband radiation (0.32 to 30.0 microns) and then subtracting the measured reflected shortwave solar radiation (0.32 to 4.0 microns), from the earth- atmosphere system. The GERB sensor is able to make measurements to within an accuracy of 1 W/sq. m. A forward model is being developed at Leicester to simulate the data from the GERB sensor for representative geophysical scenes and to investigate key parameters and processes that will affect the top of the atmosphere signal. At the heart of this model is a line-by-line radiative transfer model, the Oxford Reference Forward Model (RFM) that is to be used with model atmospheres generated from ECMWF analysis data. When MSG is launched, cloud data from the Spinning Enhanced Visible and Infrared Imager (SEVIRI), also on board, is to be used in conjunction with GERB data.
A Characterization of Movement Skills in Obese Children with and without Prader-Willi Syndrome
ERIC Educational Resources Information Center
Lam, Melanie Y.; Rubin, Daniela A.; Duran, Andrea T.; Chavoya, Frank A.; White, Elizabeth; Rose, Debra J.
2016-01-01
Purpose: The aim of this study was twofold: (a) to measure and compare motor proficiency in obese children with Prader-Willi syndrome (OB-PWS) to that in obese children without PWS (OB), and (b) to compare motor proficiency in OB-PWS and OB to normative data. Method: Motor proficiency was measured using the Bruininks-Oseretsky Test of Motor…
Kato, Shingo; Ohkuma, Moriya; Powell, Deborah H.; ...
2015-11-13
Neutrophilic microaerophilic iron-oxidizing bacteria (FeOB) are thought to play a significant role in cycling of carbon, iron and associated elements in both freshwater and marine iron-rich environments. However, the roles of the neutrophilic microaerophilic FeOB are still poorly understood due largely to the difficulty of cultivation and lack of functional gene markers. Here, we analyze the genomes of two freshwater neutrophilic microaerophilic stalk-forming FeOB, Ferriphaselus amnicola OYT1 and Ferriphaselus strain R-1. Phylogenetic analyses confirm that these are distinct species within Betaproteobacteria; we describe strain R-1 and propose the name F. globulitus. We compare the genomes to those of two freshwatermore » Betaproteobacterial and three marine Zetaproteobacterial FeOB isolates in order to look for mechanisms common to all FeOB, or just stalk-forming FeOB. The OYT1 and R-1 genomes both contain homologs to cyc2, which encodes a protein that has been shown to oxidize Fe in the acidophilic FeOB, Acidithiobacillus ferrooxidans. This c-type cytochrome common to all seven microaerophilic FeOB isolates, strengthening the case for its common utility in the Fe oxidation pathway. In contrast, the OYT1 and R-1 genomes lack mto genes found in other freshwater FeOB. OYT1 and R-1 both have genes that suggest they can oxidize sulfur species. Both have the genes necessary to fix carbon by the Calvin–Benson– Basshom pathway, while only OYT1 has the genes necessary to fix nitrogen. The stalk-forming FeOB share xag genes that may help form the polysaccharide structure of stalks. Both OYT1 and R-1 make a novel biomineralization structure, short rod-shaped Fe oxyhydroxides much smaller than their stalks; these oxides are constantly shed, and may be a vector for C, P, and metal transport to downstream environments. Lastly, our results show that while different FeOB are adapted to particular niches, freshwater and marine FeOB likely share common mechanisms for Fe oxidation electron transport and biomineralization pathways.« less
Massicotte, Frédéric; Fernandes, Julio Cesar; Martel-Pelletier, Johanne; Pelletier, Jean-Pierre; Lajeunesse, Daniel
2006-03-01
Human osteoarthritis (OA) is characterized by cartilage loss, bone sclerosis, osteophyte formation and inflammation of the synovial membrane. We previously reported that OA osteoblasts (Ob) show abnormal phenotypic characteristics possibly responsible for bone sclerosis and that two subgroups of OA patients can be identified by low or high endogenous production of prostaglandin E2 (PGE2) by OA Ob. Here, we determined that the elevated PGE2 levels in the high OA subgroup were linked with enhanced cyclooxygenase-2 (COX-2) protein levels compared to normal and low OA Ob. A linear relationship was observed between endogenous PGE2 levels and insulin-like growth factor 1 (IGF-1) levels in OA Ob. As parathyroid hormone (PTH) and PGE2 are known stimulators of IGF-1 production in Ob, we next evaluated their effect in OA Ob. Both subgroups increased their IGF-1 production similarly in response to PGE2, while the high OA subgroup showed a blunted response to PTH compared to the low OA group. Conversely, only the high OA group showed a significant inhibition of IGF-1 production when PGE2 synthesis was reduced with Naproxen, a non-steroidal antiinflammatory drug (NSAID) that inhibits cyclooxygenases (COX). The PGE2-dependent stimulation of IGF-1 synthesis was due in part to the cAMP/protein kinase A pathway since both the direct inhibition of this pathway with H-89 and the inhibition of EP2 or EP4 receptors, linked to cAMP production, reduced IGF-1 synthesis. The production of the most abundant IGF-1 binding proteins (IGFBPs) in bone tissue, IGFBP-3, -4, and -5, was lower in OA compared to normal Ob independently of the OA group. Under basal condition, OA Ob expressed similar IGF-1 mRNA to normal Ob; however, PGE2 stimulated IGF-1 mRNA expression more in OA than normal Ob. These data suggest that increased IGF-1 levels correlate with elevated endogenous PGE2 levels in OA Ob and that higher IGF-1 levels in OA Ob could be important for bone sclerosis in OA.
Effect of hypothalamic electrolytic lesions in White Leghorn and broiler male cockerels.
Arnon, E; Snapir, N; Robinzon, B; Heiblum, R
2004-06-01
1. This study compared the effect of bilateral electrolytic lesions of the basomedial hypothalamus (HL) in broiler and White Leghorn (WL) males. 2. Hypothalamic lesions were placed in WL at 10 weeks of age (body weight 1.1 kg) and in broilers, either at 6 weeks (body weight 1.5kg) or at 10 weeks of age (body weight 3.4kg). They were fed ad libitum until autopsy at 16 and 17 weeks of age for broilers and WL, respectively. 3. Hypothalamic lesions caused obesity (high percentage weight of abdominal adipose tissue) in both strains. Obese fowls with unimpaired reproductive systems were classified as OB and those with functional castration as FC (functionally castrated) or FCLC (functionally castrated with large comb). 4. All post-HL syndromes-OB, FC and FCLC-were present in WL, whereas all obese broilers (which are immature at this age) were classified as OB. 5. The percentage weight of abdominal adipose tissue in OB broilers was lower than in OB WL (3% vs 5%, respectively). 6. Daily food intake of OB broilers was higher than control at 12 to 15 weeks of age, regardless of time of placement of HL, whereas daily food intake of OB WL was significantly higher than that of control WL only during the first 2 weeks following HL. 7. Body weight of OB broilers at autopsy was 20% higher than control broilers, whereas body weight of OB WL was not significantly affected. 8. An additional group of broilers was reared to sexual maturity under food restriction until 28 weeks of age. HL were placed at 10 weeks of age (body weight 1.7 kg). Autopsy was performed after a 4-week period of ad libitum feeding. 9. There were OB as well as FC and FCLC among the HL, food-restricted broilers. Percentage weight of testes and spleen were reduced in OB fowls of both strains, but more so in OB WL. 10. Hyperphagia and weight gain were not observed during the ad libitum feeding period of those obese broilers after HL, indicating that hyperphagia and weight gain are secondary to obesity.
VIIRS captures phytoplankton vertical migration in the NE Gulf of Mexico.
Qi, Lin; Hu, Chuanmin; Barnes, Brian B; Lee, Zhongping
2017-06-01
In summer 2014, a toxic Karenia brevis bloom (red tide) occurred in the NE Gulf of Mexico, during which vertical migration of K. brevis has been observed from glider measurements. The current study shows that satellite observations from the Visible Infrared Imaging Radiometer Suite (VIIRS) can capture changes in surface reflectance and chlorophyll concentration occurring within 2h, which may be attributed this K. brevis vertical migration. The argument is supported by earlier glider measurements in the same bloom, by the dramatic changes in the VIIRS-derived surface chlorophyll, and by the consistency between the short-term reflectance changes and those reported earlier from field-measured K. brevis vertical migration. Estimates using the quasi-analytical algorithm also indicate significant increases in both total absorption coefficient and backscattering coefficient in two hours. The two observations in a day from a single polar-orbiting satellite sensor are thus shown to be able to infer phytoplankton vertical movement within a short timeframe, a phenomenon difficult to capture with other sensors as each sensor can provide at most one observation per day, and cross-sensor inconsistency may make interpretation of merged-sensor data difficult. These findings strongly support geostationary satellite missions to study short-term bloom dynamics. Copyright © 2017 Elsevier B.V. All rights reserved.
Integrated fiber optic structural health sensors for inflatable space habitats
NASA Astrophysics Data System (ADS)
Ohanian, Osgar John; Garg, Naman; Castellucci, Matthew A.
2017-04-01
Inflatable space habitats offer many advantages for future space missions; however, the long term integrity of these flexible structures is a major concern in harsh space environments. Structural Health Monitoring (SHM) of these structures is essential to ensure safe operation, provide early warnings of damage, and measure structural changes over long periods of time. To address this problem, the authors have integrated distributed fiber optic strain sensors to measure loading and to identify the occurrence and location of damage in the straps and webbing used in the structural restraint layer. The fiber optic sensors employed use Rayleigh backscatter combined with optical frequency domain reflectometry to enable measurement of strain every 0.65 mm (0.026 inches) along the sensor. The Kevlar woven straps that were tested exhibited large permanent deformation during initial cycling and continued to exhibit hysteresis thereafter, but there was a consistent linear relationship between the sensor's measurement and the actual strain applied. Damage was intentionally applied to a tensioned strap, and the distributed strain measurement clearly identified a change in the strain profile centered on the location of the damage. This change in structural health was identified at a loading that was less than half of the ultimate loading that caused a structural failure. This sensing technique will be used to enable integrated SHM sensors to detect loading and damage in future inflatable space habitat structures.
Baer, Tamara E; Milliren, Carly E; Walls, Courtney; DiVasta, Amy D
2015-10-01
To review the clinical presentation, evaluation, and management of normal-weight (NW), overweight (OW), and obese (OB) adolescent and young adult women with polycystic ovary syndrome (PCOS) during a 2-year follow-up. Retrospective chart review. One hundred seventy-three adolescent and young adult women, aged 12-22 years, diagnosed with PCOS. Demographic, health data, and laboratory measures were abstracted from 3 clinic visits: baseline and 1- and 2-year follow-up. Subjects were classified as NW, OW, or OB. Longitudinal data were analyzed using repeated-measures analysis of variance. Body mass index, self-reported concerns, and lifestyle changes. Most patients (73%) were OW or OB. Family history of type 2 diabetes was greater in OW (38%) and OB (53%) patients compared with NW (22%) patients (P = .002). Acanthosis nigricans was identified in OW (62%) and OB (21%) patients but not in NW patients (0%; P < .001). OW and OB patients had higher fasting insulin (P < .001) and lower high-density lipoprotein cholesterol (P = .005) levels than NW patients, although screening rates were low. Body mass index Z-scores decreased in both OW and OB patients over time (0.07 unit/yr, P < .001). Most patients with PCOS were OW or OB. Substantial clinical variability existed in cardiovascular disease (CVD) screening; among those screened, OW and OB patients had greater CVD risk factors. Despite self-reported concerns about weight and diabetes risk among OW and OB patients, no clinically significant change in body mass index percentile occurred. Evidence-based interventions and recommendations for screening tests are needed to address CVD risk in adolescents and young adults with PCOS. Copyright © 2015 North American Society for Pediatric and Adolescent Gynecology. Published by Elsevier Inc. All rights reserved.
Zhu, Xiaoling; Jiang, Shasha; Hu, Nan; Luo, Fuling; Dong, Hailong; Kang, Yu-Ming; Jones, Kyla R; Zou, Yunzeng; Xiong, Lize; Ren, Jun
2014-07-01
Lenalidomide (Revlimid; Selleck Chemicals, Houston, TX, USA), an analogue of thalidomide, possesses potent cytokine modulatory capacity through inhibition of cytokines such as tumour necrosis factor (TNF)-α, a cytokine pivotal for the onset and development of complications in obesity and diabetes mellitus. The present study was designed to evaluate the effect of lenalidomide on oxidative stress, protein and DNA damage in multiple organs in an ob/ob murine model of obesity. To this end, C57BL/6 lean and ob/ob obese mice were administered lenalidomide (50 mg/kg per day, p.o.) for 5 days. Oxidative stress, protein and DNA damage were assessed using the conversion of reduced glutathione (GSH) to oxidized glutathione (GSSG), carbonyl formation and Comet assay, respectively. Apoptosis was evaluated using caspase 3 activity, and levels of Bax, Bcl-2, Bip, caspase 8, caspase 9 and TNF-α were assessed using western blot analysis. Lenalidomide treatment did not affect glucose clearance in lean or ob/ob mice. Obese mice exhibited a reduced GSH/GSSG ratio in the liver, gastrocnemius skeletal muscle and small intestine, as well as enhanced protein carbonyl formation, DNA damage and caspase 3 activity in the liver, kidney, skeletal muscle and intestine; these effects were alleviated by lenalidomide, with the exception of obesity-associated DNA damage in the liver and kidney. Western blot analysis revealed elevated TNF-α, Bax, Bcl-2, Bip, caspase 8 and caspase 9 in ob/ob mice with various degrees of reversal by lenalidomide treatment. Together, these data indicate that lenalidomide protects against obesity-induced tissue injury and protein damage, possibly in association with antagonism of cytokine production and cytokine-induced apoptosis and oxidative stress. © 2014 Wiley Publishing Asia Pty Ltd.
Arrizubieta, Maite; Simón, Oihane; Williams, Trevor; Caballero, Primitivo
2016-01-01
A co-occluded binary mixture of Helicoverpa armigera nucleopolyhedrovirus genotypes HearSP1B and HearLB6 at a 1:1 ratio (HearSP1B+HearLB6) was selected for the development of a virus-based biological insecticide, which requires an efficient large-scale production system. In vivo production systems require optimization studies in each host-virus pathosystem. In the present study, the effects of larval instar, rearing density, timing of inoculation, inoculum concentration and temperature on the production of HearSP1B+HearLB6 in its homologous host were evaluated. The high prevalence of cannibalism in infected larvae (40-87%) indicated that insects require individual rearing to avoid major losses in OB production. The OB production of recently molted fifth instars (7.0 x 109 OBs/larva), combined with a high prevalence of mortality (85.7%), resulted in the highest overall OB yield (6.0 x 1011 OBs/100 inoculated larvae), compared to those of third or fourth instars. However, as inoculum concentration did not influence final OB yield, the lowest concentration, LC80 (5.5 x 106 OBs/ml), was selected. Incubation temperature did not significantly influence OB yield, although larvae maintained at 30°C died 13 and 34 hours earlier than those incubated at 26°C and 23°C, respectively. We conclude that the efficient production of HearSP1B+HearLB6 OBs involves inoculation of recently molted fifth instars with a LC80 concentration of OBs followed by individual rearing at 30°C.
Li, Zi-Lun; Ebrahimi, Behzad; Zhang, Xin; Eirin, Alfonso; Woollard, John R; Tang, Hui; Lerman, Amir; Wang, Shen-Ming; Lerman, Lilach O
2014-04-01
Obesity associated with metabolic derangements (ObM) worsens the prognosis of patients with coronary artery stenosis (CAS), but the underlying cardiac pathophysiologic mechanisms remain elusive. We tested the hypothesis that ObM exacerbates cardiomyocyte loss distal to moderate CAS. Obesity-prone pigs were randomized to four groups (n = 6 each): lean-sham, ObM-sham, lean-CAS, and ObM-CAS. Lean and ObM pigs were maintained on a 12-wk standard or atherogenic diet, respectively, and left circumflex CAS was then induced by placing local-irritant coils. Cardiac structure, function, and myocardial oxygenation were assessed 4 wk later by computed-tomography and blood oxygenation level dependent (BOLD) MRI, the microcirculation with micro-computed-tomography, and injury mechanisms by immunoblotting and histology. ObM pigs showed obesity, dyslipidemia, and insulin resistance. The degree of CAS (range, 50-70%) was similar in lean and ObM pigs, and resting myocardial perfusion and global cardiac function remained unchanged. Increased angiogenesis distal to the moderate CAS observed in lean was attenuated in ObM pigs, which also showed microvascular dysfunction and increased inflammation (M1-macrophages, TNF-α expression), oxidative stress (gp91), hypoxia (BOLD-MRI), and fibrosis (Sirius-red and trichrome). Furthermore, lean-CAS showed increased myocardial autophagy, which was blunted in ObM pigs (downregulated expression of unc-51-like kinase-1 and autophagy-related gene-12; P < 0.05 vs. lean CAS) and associated with marked apoptosis. The interaction diet xstenosis synergistically inhibited angiogenic, autophagic, and fibrogenic activities. ObM exacerbates structural and functional myocardial injury distal to moderate CAS with preserved myocardial perfusion, possibly due to impaired cardiomyocyte turnover.
A SEARCH FOR STELLAR-MASS BLACK HOLES VIA ASTROMETRIC MICROLENSING
DOE Office of Scientific and Technical Information (OSTI.GOV)
Lu, J. R.; Sinukoff, E.; Ofek, E. O.
While dozens of stellar-mass black holes (BHs) have been discovered in binary systems, isolated BHs have eluded detection. Their presence can be inferred when they lens light from a background star. We attempt to detect the astrometric lensing signatures of three photometrically identified microlensing events, OGLE-2011-BLG-0022, OGLE-2011-BLG-0125, and OGLE-2012-BLG-0169 (OB110022, OB110125, and OB120169), located toward the Galactic Bulge. These events were selected because of their long durations, which statistically favors more massive lenses. Astrometric measurements were made over one to two years using laser-guided adaptive optics observations from the W. M. Keck Observatory. Lens model parameters were first constrained bymore » the photometric light curves. The OB120169 light curve is well fit by a single-lens model, while both OB110022 and OB110125 light curves favor binary lens models. Using the photometric fits as prior information, no significant astrometric lensing signal was detected and all targets were consistent with linear motion. The significant lack of astrometric signal constrains the lens mass of OB110022 to 0.05–1.79 M {sub ⊙} in a 99.7% confidence interval, which disfavors a BH lens. Fits to OB110125 yielded a reduced Einstein crossing time and insufficient observations during the peak, so no mass limits were obtained. Two degenerate solutions exist for OB120169, which have a lens mass between 0.2–38.8 M {sub ⊙} and 0.4–39.8 M {sub ⊙} for a 99.7% confidence interval. Follow-up observations of OB120169 will further constrain the lens mass. Based on our experience, we use simulations to design optimal astrometric observing strategies and show that with more typical observing conditions the detection of BHs is feasible.« less
Chen, H-L; Tung, Y-T; Tsai, C-L; Lai, C-W; Lai, Z-L; Tsai, H-C; Lin, Y-L; Wang, C-H; Chen, C-M
2014-09-01
Fatty liver disease is commonly associated with obesity, insulin resistance and diabetes. Severe fatty liver is sometimes accompanied by steatohepatitis and may lead to the development of hepatocellular carcinoma. At present, there is no effective treatment for non-alcoholic fatty liver disease (NAFLD); thus, recent investigations have focused on developing effective therapeutics to treat this condition. This study aimed to evaluate the effects of kefir on the hepatic lipid metabolism of ob/ob mice, which are commonly used to model fatty liver disease. In this study, we used leptin receptor-deficient ob/ob mice as an animal disease model of NAFLD. Six-week-old ob/ob mice were orally administered the dairy product kefir (140 mg kg(-1) of body weight (BW) per day) for 4 weeks. The data demonstrated that kefir improved fatty liver syndrome on BW, energy expenditure and basal metabolic rate by inhibiting serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities (P<0.05) and by decreasing the triglyceride (TG) and total cholesterol (TC) contents of the liver (P<0.05). Oral kefir administration also significantly reduced the macrovesicular fat quantity in liver tissue. In addition, kefir markedly decreased the expression of the genes sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) (P<0.05) but not the expression of peroxisome proliferator-activated receptor α (PPARα) or hepatic carnitine palmitoyltransferase-1α (CPT1α) in the livers of ob/ob mice. On the basis of these results, we conclude that kefir improves NAFLD on BW, energy expenditure and basal metabolic rate by inhibiting the lipogenesis pathway and that kefir may have the potential for clinical application to the prevention or treatment of NAFLD.
Fujisawa, Manabu; Seike, Keisuke; Fukumoto, Kouta; Suehara, Yasuhito; Fukaya, Masafumi; Sugihara, Hiroki; Takeuchi, Masami; Matsue, Kosei
2014-01-01
The emergence of oligoclonal bands (OB) has been reported in patients with multiple myeloma (MM) after stem cell transplantation (SCT) or successful chemotherapy. However, their clinical relevance remains unclear. We reviewed the clinical records of MM patients from January 2006 to May 2014. Treatment response was evaluated by International Working Group (IMWG) criteria. Serum immunofixation tests were performed at least every 3 months if the patient achieved more than very good partial response (VGPR). Free light chain (FLC) and minimal residual disease measurement by multicolor flow cytometry (MFC) were performed to evaluate the response to treatment. Among the 163 patients included in the study, 40 developed OB. Detection rates of OB in patients with complete response (CR), VGPR and partial response (PR) or less were 51.8, 36.3 and 0%, respectively. Patients with OB showed better progression-free survival (PFS) and overall survival (OS) rates than those without OB (P = 0.028 and P < 0.001, respectively). However, if the patients were limited to ≥VGPR or CR, development of OB did not affect PFS (P = 0.621 and P = 0.646, respectively) or OS (P = 0.189 and P = 0.766, respectively). OB was observed in 60% of patients after SCT, and in 36.6% of patients with more than VGPR without SCT (P < 0.001). Patients with OB tended to have less minimal residual disease than those without OB (P = 0.054) and its presence may affect the stringent CR criteria. In conclusion, the emergence of OB was seen exclusively in patients with favorable responses, but its emergence per se could not be translated to improved survival. PMID:25182124
Fujisawa, Manabu; Seike, Keisuke; Fukumoto, Kouta; Suehara, Yasuhito; Fukaya, Masafumi; Sugihara, Hiroki; Takeuchi, Masami; Matsue, Kosei
2014-11-01
The emergence of oligoclonal bands (OB) has been reported in patients with multiple myeloma (MM) after stem cell transplantation (SCT) or successful chemotherapy. However, their clinical relevance remains unclear. We reviewed the clinical records of MM patients from January 2006 to May 2014. Treatment response was evaluated by International Working Group (IMWG) criteria. Serum immunofixation tests were performed at least every 3 months if the patient achieved more than very good partial response (VGPR). Free light chain (FLC) and minimal residual disease measurement by multicolor flow cytometry (MFC) were performed to evaluate the response to treatment. Among the 163 patients included in the study, 40 developed OB. Detection rates of OB in patients with complete response (CR), VGPR and partial response (PR) or less were 51.8, 36.3 and 0%, respectively. Patients with OB showed better progression-free survival (PFS) and overall survival (OS) rates than those without OB (P = 0.028 and P < 0.001, respectively). However, if the patients were limited to ≥VGPR or CR, development of OB did not affect PFS (P = 0.621 and P = 0.646, respectively) or OS (P = 0.189 and P = 0.766, respectively). OB was observed in 60% of patients after SCT, and in 36.6% of patients with more than VGPR without SCT (P < 0.001). Patients with OB tended to have less minimal residual disease than those without OB (P = 0.054) and its presence may affect the stringent CR criteria. In conclusion, the emergence of OB was seen exclusively in patients with favorable responses, but its emergence per se could not be translated to improved survival. © 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.
Finger, Beate C; Schellekens, Harriët; Dinan, Timothy G; Cryan, John F
2011-08-01
Several fine-tuned and interconnected hypothalamic peptidergic systems orchestrate the regulation of energy homeostasis in the body. The orexigenic peptide ghrelin and the anorexigenic peptide leptin are among the most important, and both have been implicated in the development of eating disorders from obesity to anorexia nervosa. The goal of these studies was to examine the response of leptin-deficient ob/ob mice in ghrelin-receptor ligands in a food intake task. Changes in cumulative food intake were measured after peripheral administration of ghrelin (1 and 2 nmol/10 g) and the ghrelin-receptor antagonist (D-Lys(3))-GHRP-6 (66.6 and 133.3 nmol/10 g) in obese and lean control mice during the light and dark cycle as well as in a state of food restriction. Hypothalamic ghrelin and ghrelin-receptor expression was measured in ob/ob and lean mice at two different timepoints. Ghrelin increased food intake in lean and obese mice in the light and dark cycle, whereas the ghrelin-receptor antagonist caused significantly stronger reduction in food intake in obese mice only in the dark cycle. After fasting, ob/ob mice displayed decreased light cycle sensitivity to the anorexigenic effects of the ghrelin-receptor antagonist. Hypothalamic expression levels of ghrelin were unaltered during the light cycle but decreased during the dark cycle in ob/ob mice; whereas, although unchanged in the light cycle, ghrelin-receptor expression was increased in the dark cycle in obese mice. The functionality and sensitivity of the ghrelinergic system is dependent on the time of day and the satiety state in leptin-deficient ob/ob mice.
Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-).
Martins, Fabiane Ferreira; Aguila, Marcia Barbosa; Mandarim-de-Lacerda, Carlos Alberto
2017-06-01
The obesity and its comorbidities, including resistance to leptin, impacts the reproductive function. Testes express leptin receptors in the germ cells and Leydig cells. Then, leptin-deficient animals are obese and infertile. We aimed to evaluate the structure and steroidogenic pathway of the testis of deficient leptin mice. Three months old male C57BL/6 mice (wild-type, WT) and deficient leptin (ob/ob) mice had their testes dissected and prepared for analyses. Compared to the WT group, the ob/ob group showed a greater body mass with smaller testes, and alterations in the germinative epithelium: fewer spermatogonia, spermatocytes, and spermatids. The Sertoli cells and the germ cells showed condensed nuclei and nuclear fragmentation indicating cell death, in agreement with a low expression of the proliferating cell nuclear antigen and a high expression of Caspase3. In the ob/ob group, the sperm was absent in the seminiferous tubules, and the steroidogenic pathway was compromised (low 3Beta hydroxysteroid dehydrogenase and steroidogenic acute regulatory protein). Further, all hormone receptors involved in the testicular function were down expressed (androgen, estrogen, follicle-stimulating, luteinizing, aromatase, and nicotinamide adenine dinucleotide phosphate). In conclusion, the findings indicate significant morphological, hormonal and enzymatic changes in the testis of the ob/ob mice. The shifts in the enzymatic steroidogenic pathway and the enzymes related to spermatic activity support the insights about the failures in the fertility of these animals. The study provides new evidence and contributes to the understanding of how the lack of leptin and obesity might negatively modulate the testicular function leading to infertility. Copyright © 2017 Elsevier GmbH. All rights reserved.
Arrizubieta, Maite; Simón, Oihane; Williams, Trevor; Caballero, Primitivo
2016-01-01
A co-occluded binary mixture of Helicoverpa armigera nucleopolyhedrovirus genotypes HearSP1B and HearLB6 at a 1:1 ratio (HearSP1B+HearLB6) was selected for the development of a virus-based biological insecticide, which requires an efficient large-scale production system. In vivo production systems require optimization studies in each host-virus pathosystem. In the present study, the effects of larval instar, rearing density, timing of inoculation, inoculum concentration and temperature on the production of HearSP1B+HearLB6 in its homologous host were evaluated. The high prevalence of cannibalism in infected larvae (40–87%) indicated that insects require individual rearing to avoid major losses in OB production. The OB production of recently molted fifth instars (7.0 x 109 OBs/larva), combined with a high prevalence of mortality (85.7%), resulted in the highest overall OB yield (6.0 x 1011 OBs/100 inoculated larvae), compared to those of third or fourth instars. However, as inoculum concentration did not influence final OB yield, the lowest concentration, LC80 (5.5 x 106 OBs/ml), was selected. Incubation temperature did not significantly influence OB yield, although larvae maintained at 30°C died 13 and 34 hours earlier than those incubated at 26°C and 23°C, respectively. We conclude that the efficient production of HearSP1B+HearLB6 OBs involves inoculation of recently molted fifth instars with a LC80 concentration of OBs followed by individual rearing at 30°C. PMID:27732657
Altered insulin response to an acute bout of exercise in pediatric obesity.
Tran, Brian D; Leu, Szu-Yun; Oliver, Stacy; Graf, Scott; Vigil, Diana; Galassetti, Pietro
2014-11-01
Pediatric obesity typically induces insulin resistance, often later evolving into type 2 diabetes. While exercise, enhancing insulin sensitivity, is broadly used to prevent this transition, it is unknown whether alterations in the exercise insulin response pattern occur in obese children. Therefore, we measured exercise insulin responses in 57 healthy weight (NW), 20 overweight (OW), and 56 obese (Ob) children. Blood samples were drawn before and after 30 min of intermittent (2 min on, 1 min off) cycling at ~80% VO2max. In a smaller group (14 NW, 6 OW, 15 Ob), a high-fat meal was ingested 45 min preexercise. Baseline glycemia was similar and increased slightly and similarly in all groups during exercise. Basal insulin (pmol/L) was significantly higher in Ob vs. other groups; postexercise, insulin increased in NW (+7± 3) and OW (+5 ± 8), but decreased in Ob (-15±5, p < .0167 vs. NW). This insulin drop in Ob was disproportionately more pronounced in the half of Ob children with higher basal insulin (Ob-H). In all groups, high-fat feeding caused a rapid rise in insulin, promptly corrected by exercise. In Ob, however, insulin rose again 30 min postexercise. Our data indicates a distinct pattern of exercise-induced insulin modulation in pediatric obesity, possibly modulated by basal insulin concentrations.
A Fully Implantable, NFC Enabled, Continuous Interstitial Glucose Monitor
Anabtawi, Nijad; Freeman, Sabrina; Ferzli, Rony
2017-01-01
This work presents an integrated system-on-chip (SoC) that forms the core of a long-term, fully implantable, battery assisted, passive continuous glucose monitor. It integrates an amperometric glucose sensor interface, a near field communication (NFC) wireless front-end and a fully digital switched mode power management unit for supply regulation and on board battery charging. It uses 13.56 MHz (ISM) band to harvest energy and backscatter data to an NFC reader. System was implemented in 14nm CMOS technology and validated with post layout simulations. PMID:28702512
A Fully Implantable, NFC Enabled, Continuous Interstitial Glucose Monitor.
Anabtawi, Nijad; Freeman, Sabrina; Ferzli, Rony
2016-02-01
This work presents an integrated system-on-chip (SoC) that forms the core of a long-term, fully implantable, battery assisted, passive continuous glucose monitor. It integrates an amperometric glucose sensor interface, a near field communication (NFC) wireless front-end and a fully digital switched mode power management unit for supply regulation and on board battery charging. It uses 13.56 MHz (ISM) band to harvest energy and backscatter data to an NFC reader. System was implemented in 14nm CMOS technology and validated with post layout simulations.
Satellite microwave observations of the Utah Great Salt Lake Desert
NASA Technical Reports Server (NTRS)
Ulaby, F. T.; Dellwig, L. F.; Schmugge, T.
1975-01-01
Microwave data acquired over the Great Salt Lake Desert area by sensors aboard Skylab and Nimbus 5 indicate that the microwave emission and backscatter were strongly influenced by contributions from subsurface layers of sediment saturated with brine. This phenomenon was observed by Skylab's S-194 radiometer operating at 1.4 GHz, S-193 RADSCAT (Radiometer-Scatterometer) operating at 13.9 GHz, and the Nimbus 5 ESMR (Electrically Scanning Microwave Radiometer) operating at 19.35 GHz. The availability of ESMR data over an 18-month period allowed an investigation of temporal variations.
NASA Astrophysics Data System (ADS)
Zuo, Li-qin; Lu, Yong-jun; Wang, Ya-ping; Liu, Huai-xiang
2014-06-01
In order to study the mechanism of flow-sediment movement, it is essential to obtain measured data of water hydrodynamic and sediment concentration process with high spatial and temporal resolution in the bottom boundary layer (BBL). Field observations were carried out in the northwest Caofeidian sea area in the Bohai Bay. Near 2 m isobath (under the lowest tidal level), a tripod system was installed with AWAC (Acoustic Wave And Current), ADCP (Acoustic Doppler Current Profilers), OBS-3A (Optical Backscatter Point Sensor), ADV (Acoustic Doppler Velocimeters), etc. The accurate measurement of the bottom boundary layer during a single tidal period was carried out, together with a long-term sediment concentration measurement under different hydrological conditions. All the measured data were used to analyze the characteristics of wave-current-sediment movement and the BBL. Analysis was performed on flow structure, shear stress, roughness, eddy viscosity and other parameters of the BBL. Two major findings were made. Firstly, from the measured data, the three-layer distribution model of the velocity profiles and eddy viscosities in the wave-current BBL are proposed in the observed sea area; secondly, the sediment movement is related closely to wind-waves in the muddy coast area where sediment is clayey silt: 1) The observed suspended sediment concentration under light wind conditions is very low, with the peak value generally smaller than 0.1 kg/m3 and the average value being 0.03 kg/m3; 2) The sediment concentration increases continuously under the gales over 6-7 in Beaufort scale, under a sustained wind action. The measured peak sediment concentration at 0.4 m above the seabed is 0.15-0.32 kg/m3, and the average sediment concentration during wind-wave action is 0.08-0.18 kg/m3, which is about 3-6 times the value under light wind conditions. The critical wave height signaling remarkable changes of sediment concentration is 0.5 m. The results show that the suspended load sediment concentration is mainly influenced by wave-induced sediment suspension.
Near-bed observations of high-concentration sediment transport in the Changjiang Estuary
NASA Astrophysics Data System (ADS)
Zhou, Z.; Ge, J.; Ding, P.
2017-12-01
The North Passage, the core of turbidity maximum in the Changjiang Estuary, is now under the strong sedimentation due to the abundant sediment supply from the upstream Changjiang River and the river-tide interacted dynamics. Recent studies suggested that strong siltation could be attributed to bottom high-concentration sediment transport, which however is very difficult to be detected and observed by vessel-anchored survey methods. To better understand the mechanisms of sediment transport and deposition in the channel region of the North Passage and its adjacent areas, we conducted continuous field observations which covered spring and neap tide period in the wintertime of 2016, the summertime of 2015 and 2017, focusing on near-bottom sediment transport. Tripods mounted with multiple instruments, including up-looking and down-looking Acoustic Doppler Current Profilers(ADCP), Vector Current Meter(ADV), Optical Backscatter Sensor(OBS), ASM, ALEC and RBR were used to observe the near-bottom physical process and its induced sediment dynamics. Results of these observations clearly described the current-wave-sediment interaction, which produced different patterns of bottom mud suspension at different tripods. Both hydrodynamic features and suspended sediment showed variations between spring and neap tide. Taking data of 2016 as an example, averaged suspended sediment concentration(SSC) at two tripods was 1.52 g/L and 2.13 g/L during the neap tide, 4.51 g/L and 5.75 g/L with the peak value reaching 25 g/L during the spring tide. At the tripod which was closer to the channel region, three peaks of SSC during the spring tide occurred near the flood slack with notable salinity increase, indicating the impact of saltwater intrusion on the bottom hydrodynamics. The results showed the occurrence of high-concentration suspended sediment was probably related to combined effects of bottom salinity intrusion, turbulent kinetic energy(TKE) and local stratification due to density gradient from intruded salinity and local sediment suspension. Meanwhile, tripods' monitoring identified a significant cross-channel component of residual current, which could produce potential bottom sediment accumulation in the channel region within the North Passage.
A Novel Phase Sensitive Quantum Well Nanostructure Scheme for Controlling Optical Bistability
NASA Astrophysics Data System (ADS)
Raheli, Ali
2018-04-01
A novel four-level lambda-type quantum well (QW) nanostructure is proposed based on phase sensitive optical bistability (OB) and multistability (OM) with a closed-loop configuration. The influence of controlling parameters of the system on OB and OM is investigated. In particular, it is found that the OB behavior is strongly sensitive to the relative phase of applied fields. It is also shown that under certain parametric conditions, the OB can be switched to OM or vice versa. The controllability of OB/OM in such a QW nanostructure may bring some new possibilities for technological applications in solid-state quantum information science and optoelectronics.
Tobe, Seiichi; Hoshi, Marika; Iizuka, Kinue; Tadenuma, Hirohiko; Takaoka, Hiromitsu; Komoriya, Tomoe; Kohno, Hideki
2012-01-01
Noroviruses (NVs) are major causative pathogens of gastroenteritis. The disinfection of contaminated clothing during common household washing is desirable. The virucidal effects of 2 bleach activators, sodium alkyl acyloxybenzene sulfonate (OBS) and alkyl acyloxybenzoic acid (OBC), were studied using Feline calicivirus (FCV) as a surrogate for NVs. FCV was added to solutions containing either OBS or OBC and sodium percarbonate at various temperatures and for varying lengths of time. OBS and OBC, which generate long carbon chain peroxy acids, enhanced the virucidal effect of sodium percarbonate (PC). In particular, sodium lauroyloxybenzene sulfonate (OBS-12) and decanoyloxybenzoic acid (OBC-10) showed superior virucidal effects. Although the virucidal effect of 38-200 mg/L OBS-12 was maintained with 2-5% (v/v) horse serum, there was less of an effect with the same concentration of available chlorine. OBS and OBC have been used as ingredients in some laundry products to increase bleaching activity. It is expected that the use of OBS and OBC is also effective for the inactivation of NVs under common household washing conditions.
Initial Verification of GEOS-4 Aerosols Using CALIPSO and MODIS: Scene Classification
NASA Technical Reports Server (NTRS)
Welton, Ellsworth J.; Colarco, Peter R.; Hlavka, Dennis; Levy, Robert C.; Vaughan, Mark A.; daSilva, Arlindo
2007-01-01
A-train sensors such as MODIS and MISR provide column aerosol properties, and in the process a means of estimating aerosol type (e.g. smoke vs. dust). Correct classification of aerosol type is important because retrievals are often dependent upon selection of the right aerosol model. In addition, aerosol scene classification helps place the retrieved products in context for comparisons and analysis with aerosol transport models. The recent addition of CALIPSO to the A-train now provides a means of classifying aerosol distribution with altitude. CALIPSO level 1 products include profiles of attenuated backscatter at 532 and 1064 nm, and depolarization at 532 nm. Backscatter intensity, wavelength ratio, and depolarization provide information on the vertical profile of aerosol concentration, size, and shape. Thus similar estimates of aerosol type using MODIS or MISR are possible with CALIPSO, and the combination of data from all sensors provides a means of 3D aerosol scene classification. The NASA Goddard Earth Observing System general circulation model and data assimilation system (GEOS-4) provides global 3D aerosol mass for sulfate, sea salt, dust, and black and organic carbon. A GEOS-4 aerosol scene classification algorithm has been developed to provide estimates of aerosol mixtures along the flight track for NASA's Geoscience Laser Altimeter System (GLAS) satellite lidar. GLAS launched in 2003 and did not have the benefit of depolarization measurements or other sensors from the A-train. Aerosol typing from GLAS data alone was not possible, and the GEOS-4 aerosol classifier has been used to identify aerosol type and improve the retrieval of GLAS products. Here we compare 3D aerosol scene classification using CALIPSO and MODIS with the GEOS-4 aerosol classifier. Dust, smoke, and pollution examples will be discussed in the context of providing an initial verification of the 3D GEOS-4 aerosol products. Prior model verification has only been attempted with surface mass comparisons and column optical depth from AERONET and MODIS.
Development of an electromagnetic imaging system for well bore integrity inspection
NASA Astrophysics Data System (ADS)
Plotnikov, Yuri; Wheeler, Frederick W.; Mandal, Sudeep; Climent, Helene C.; Kasten, A. Matthias; Ross, William
2017-02-01
State-of-the-art imaging technologies for monitoring the integrity of oil and gas well bores are typically limited to the inspection of metal casings and cement bond interfaces close to the first casing region. The objective of this study is to develop and evaluate a novel well-integrity inspection system that is capable of providing enhanced information about the flaw structure and topology of hydrocarbon producing well bores. In order to achieve this, we propose the development of a multi-element electromagnetic (EM) inspection tool that can provide information about material loss in the first and second casing structure as well as information about eccentricity between multiple casing strings. Furthermore, the information gathered from the EM inspection tool will be combined with other imaging modalities (e.g. data from an x-ray backscatter imaging device). The independently acquired data are then fused to achieve a comprehensive assessment of integrity with greater accuracy. A test rig composed of several concentric metal casings with various defect structures was assembled and imaged. Initial test results were obtained with a scanning system design that includes a single transmitting coil and several receiving coils mounted on a single rod. A mechanical linear translation stage was used to move the EM sensors in the axial direction during data acquisition. For simplicity, a single receiving coil and repetitive scans were employed to simulate performance of the designed receiving sensor array system. The resulting electromagnetic images enable the detection of the metal defects in the steel pipes. Responses from several sensors were used to assess the location and amount of material loss in the first and second metal pipe as well as the relative eccentric position between these two pipes. The results from EM measurements and x-ray backscatter simulations demonstrate that data fusion from several sensing modalities can provide an enhanced assessment of flaw structures in producing well bores and potentially allow for early detection of anomalies that if undetected might lead to catastrophic failures.
The CIDA Variability Survey of Orion OB1. I. The Low-Mass Population of Ori OB1a and 1b
NASA Astrophysics Data System (ADS)
Briceño, Cesar; Calvet, Nuria; Hernández, J.; Vivas, A. K.; Hartmann, Lee; Downes, J. J.; Berlind, Perry
2005-02-01
We present results of a large-scale, multiepoch optical survey of the Orion OB1 association, carried out with the QUEST camera at the Venezuela National Astronomical Observatory. We identify for the first time the widely spread low-mass, young population in the Ori OB1a and OB1b subassociations. Candidate members were picked up by their variability in the V band and position in color-magnitude diagrams. We obtained spectra to confirm membership. In a region spanning ~68 deg2, we found 197 new young stars; of these, 56 are located in the Ori OB1a subassociation and 141 in Ori OB1b. The spatial distribution of the low-mass young stars is spatially coincident with that of the high-mass members but suggests a much sharper edge to the association. Comparison with the spatial extent of molecular gas and extinction maps indicates that the subassociation Ori OB1b is concentrated within a ringlike structure of radius ~2°(~15 pc at 440 pc), centered roughly on the star ɛ Ori in the Orion belt. The ring is apparent in 13CO and corresponds to a region with an extinction AV>=1. The stars exhibiting strong Hα emission, an indicator of active accretion, are found along this ring, whereas the center is populated with weak Hα-emitting stars. In contrast, Ori OB1a is located in a region devoid of gas and dust. We identify a grouping of stars within a ~3 deg2 area located in Ori OB1a, roughly clustered around the B2 star 25 Ori. The Herbig Ae/Be star V346 Ori is also associated with this grouping, which could be an older analog of σ Ori. Using several sets of evolutionary tracks, we find an age of 7-10 Myr for Ori OB1a and of ~4-6 Myr for Ori OB1b, consistent with previous estimates from OB stars. Indicators such as the equivalent width of Hα and near-IR excesses show that the number of accreting low-mass stars decreases sharply between Ori OB1b and Ori OB1a. These results indicate that although a substantial fraction of accreting disks remain at ages ~5 Myr, inner disks are essentially dissipated by 10 Myr. Based on observations obtained at the Llano del Hato National Astronomical Observatory of Venezuela, operated by Centro de Investigaciones de Astronomía (CIDA) for the Ministerio de Ciencia y Tecnología, and at the Fred Lawrence Whipple Observatory (FLWO) of the Smithsonian Institution. Based on observations obtained at the 3.5 m WIYN Telescope. The WIYN Observatory is a joint facility of the University of Wisconsin-Madison, Indiana University, Yale University, and the National Optical Astronomy Observatory (NOAO).
Zhou, Yi; Yu, Xueqing; Chen, Huimei; Sjöberg, Sara; Roux, Joséphine; Zhang, Lijun; Ivoulsou, Al-Habib; Bensaid, Farid; Liu, Conglin; Liu, Jian; Tordjman, Joan; Clement, Karine; Lee, Chih-Hao; Hotamisligil, Gokhan S.; Libby, Peter; Shi, Guo-Ping
2015-01-01
SUMMARY Mast cells (MCs) contribute to the pathogenesis of obesity and diabetes. This study demonstrates that leptin deficiency slants MCs toward anti-inflammatory functions. MCs in the white adipose tissues (WAT) of lean humans and mice express negligible leptin. Adoptive transfer of leptin-deficient MCs expanded ex vivo mitigates diet-induced and pre-established obesity and diabetes in mice. Mechanistic studies show that leptin-deficient MCs polarize macrophages from M1 to M2 functions because of impaired cell signaling and an altered balance between pro- and anti-inflammatory cytokines, but do not affect T-cell differentiation. Rampant body weight gain in ob/ob mice, a strain that lacks leptin, associates with reduced MC content in WAT. In ob/ob mice, genetic depletion of MCs exacerbates obesity and diabetes, and repopulation of ex vivo expanded ob/ob MCs ameliorates these diseases. PMID:26481668
Large-scale magnetic topologies of early M dwarfs
NASA Astrophysics Data System (ADS)
Donati, J.-F.; Morin, J.; Petit, P.; Delfosse, X.; Forveille, T.; Aurière, M.; Cabanac, R.; Dintrans, B.; Fares, R.; Gastine, T.; Jardine, M. M.; Lignières, F.; Paletou, F.; Ramirez Velez, J. C.; Théado, S.
2008-10-01
We present here additional results of a spectropolarimetric survey of a small sample of stars ranging from spectral type M0 to M8 aimed at investigating observationally how dynamo processes operate in stars on both sides of the full convection threshold (spectral type M4). The present paper focuses on early M stars (M0-M3), that is above the full convection threshold. Applying tomographic imaging techniques to time series of rotationally modulated circularly polarized profiles collected with the NARVAL spectropolarimeter, we determine the rotation period and reconstruct the large-scale magnetic topologies of six early M dwarfs. We find that early-M stars preferentially host large-scale fields with dominantly toroidal and non-axisymmetric poloidal configurations, along with significant differential rotation (and long-term variability); only the lowest-mass star of our subsample is found to host an almost fully poloidal, mainly axisymmetric large-scale field resembling those found in mid-M dwarfs. This abrupt change in the large-scale magnetic topologies of M dwarfs (occurring at spectral type M3) has no related signature on X-ray luminosities (measuring the total amount of magnetic flux); it thus suggests that underlying dynamo processes become more efficient at producing large-scale fields (despite producing the same flux) at spectral types later than M3. We suspect that this change relates to the rapid decrease in the radiative cores of low-mass stars and to the simultaneous sharp increase of the convective turnover times (with decreasing stellar mass) that models predict to occur at M3; it may also be (at least partly) responsible for the reduced magnetic braking reported for fully convective stars. Based on observations obtained at the Télescope Bernard Lyot (TBL), operated by the Institut National des Science de l'Univers of the Centre National de la Recherche Scientifique of France. E-mail: donati@ast.obs-mip.fr (J-FD); jmorin@ast.obs-mip.fr (JM); petit@ast.obs-mip.fr (PP); xavier.delfosse@obs.ujf-grenoble.fr (XD); thierry.forveille@obs.ujf-grenoble.fr (TF); auriere@ast.obs-mip.fr (MA); remi.cabanac@ast.obs-mip.fr (RC); dintrans@ast.obs-mip.fr (BD); rfares@ast.obs-mip.fr (RF); tgastine@ast.obs-mip.fr (TG); mmj@st-and.ac.uk (MMJ); lignieres@ast.obs-mip.fr (FL); fpaletou@ast.obs-mip.fr (FP); julio.ramirez@obspm.fr (JCRV); sylvie.theado@ast.obs-mip.fr (ST)
Defensive medicine among obstetricians and gynaecologists in Turkey.
Küçük, Mert
2018-02-01
In recent years, there has been a remarkable increase in medical malpractice litigations against OB/GYNs in Turkey and globally. This high litigation atmosphere may have changed attitudes, behaviour and practice of OB/GYNs. In the current study, opinions and attitudes of OB/GYNs regarding defensive medicine and to what extent they practice it were investigated. One hundred and eight OB/GYNs participated in the study. All participants found obstetrics and gynaecology riskier when compared with other medical branches and reported that they were increasingly practising defensive medicine. The majority of the OB/GYNs stated that they abstained from many risky interventions and expressed their belief that the high caesarean section (C-section) rate was associated with medico-legal concerns. The majority of the participants supported enacting of a specific medical malpractice law and supported the establishment of medically specialised courts. These regulations demanded by OB/GYNs should be taken into account by health authorities. Impact statement What is already known on this subject: In recent years, there has been a remarkable increase in medical malpractice litigations against OB/GYNs in Turkey and globally. Turkey has serious problems with the high C-section rate, which has been suggested to be related to medicolegal issues in a previous research. Fifty-one percent of babies, namely most of them, are delivered via C-section. There is no specific medical malpractice law and medically specialised court in Turkey. What the results of this study add: It seems like there is a professional liability crisis among OB/GYNs in Turkey. OB/GYNs reported that they were increasingly practising defensive medicine, and stated that they abstained from many risky interventions. A high C-section rate was found to be related to medicolegal concerns in OB/GYNs' perspective in the current study. OB/GYNs demanded some reasonable regulations. What the implications are of these findings for clinical practice and/or further research: Regulations demanded by OB/GYNs, which were probed in the current study, such as enacting a specific medical malpractice law and establishment of a medically specialised court, should be taken into account by health authorities in Turkey. The findings of the current study is believed to produce important results for the success of Health transformation programme put into practice in Turkey, which was not able to stop increasing C-section rates. Studies evaluating the direct or indirect costs related to defensive medicine practices of OB/GYNs in Turkey should be performed in subsequent research.
Blažetić, Senka; Labak, Irena; Viljetić, Barbara; Balog, Marta; Vari, Sandor G.; Krivošíková, Zora; Gajdoš, Martin; Kramárová, Patrícia; Kebis, Anton; Vuković, Rosemary; Puljak, Livia; Has-Schön, Elizabeta; Heffer, Marija
2014-01-01
Aim To evaluate in a rat animal model whether ovariectomy, high fat diet (HFD), and physical activity in the form of running affect leptin receptor (Ob-R) distribution in the brain and white fat tissue compared to sham (Sh) surgery, standard diet (StD), and sedentary conditions. Methods The study included 48 female laboratory Wistar rats (4 weeks old). Following eight weeks of feeding with standard or HFD, rats were subjected to either OVX or Sh surgery. After surgery, all animals continued StD or HFD for the next 10 weeks. During these 10 weeks, ovariectomy and Sh groups were subjected to physical activity or sedentary conditions. Free-floating immunohistochemistry and Western blot methods were carried out to detect Ob-R in the brain and adipose tissue. Results StD-ovariectomy-sedentary group had a greater number of Ob-R positive neurons in lateral hypothalamic nuclei than StD-Sh-sedentary group. There was no difference in Ob-R positive neurons in arcuatus nuclei between all groups. Ob-R distribution in the barrel cortex was higher in HFD group than in StD group. Ob-R presence in perirenal and subcutaneous fat was decreased in StD-ovariectomy group. Conclusion HFD and ovariectomy increased Ob-R distribution in lateral hypothalamic nuclei, but there was no effect on arcuatus nuclei. Our results are first to suggest that HFD, ovariectomy, and physical activity affect Ob-R distribution in the barrel cortex, which might be correlated with the role of Ob-R in election of food in rats. PMID:24891281
Leptin regulates ACE activity in mice.
Hilzendeger, Aline Mourao; Morais, Rafael Leite; Todiras, Mihail; Plehm, Ralph; da Costa Goncalves, Andrey; Qadri, Fatimunnisa; Araujo, Ronaldo Carvalho; Gross, Volkmar; Nakaie, Clovis Ryuichi; Casarini, Dulce Elena; Carmona, Adriana Karaoglanovic; Bader, Michael; Pesquero, João Bosco
2010-09-01
Leptin is a hormone related to metabolism. It also influences blood pressure, but the mechanisms triggered in this process are not yet elucidated. Angiotensin-I converting enzyme (ACE) regulates cardiovascular functions and recently has been associated with metabolism control and obesity. Here, we used ob/ob mice, a model lacking leptin, to answer the question whether ACE and leptin could interact to influence blood pressure, thereby linking the renin-angiotensin system and obesity. These mice are obese and diabetic but have normal 24 h mean arterial pressure. Our results show that plasma and lung ACE activities as well as ACE mRNA expression were significantly decreased in ob/ob mice. In agreement with these findings, the hypotensive effect produced by enalapril administration was attenuated in the obese mice. Plasma renin, angiotensinogen, angiotensin I, bradykinin, and angiotensin 1-7 were increased, whereas plasma angiotensin II concentration was unchanged in obese mice. Chronic infusion of leptin increased renin activity and angiotensin II concentration in both groups and increased ACE activity in ob/ob mice. Acute leptin infusion restored ACE activity in leptin-deficient mice. Moreover, the effect of an ACE inhibitor on blood pressure was not changed in ob/+ mice during leptin treatment but increased four times in obese mice. In summary, our findings show that the renin-angiotensin system is altered in ob/ob mice, with markedly reduced ACE activity, which suggests a possible connection between the renin-angiotensin system and leptin. These results point to an important interplay between the angiotensinergic and the leptinergic systems, which may play a role in the pathogenesis of obesity, hypertension, and metabolic syndrome.
Blažetić, Senka; Labak, Irena; Viljetić, Barbara; Balog, Marta; Vari, Sandor G; Krivošíková, Zora; Gajdoš, Martin; Kramárová, Patrícia; Kebis, Anton; Vuković, Rosemary; Puljak, Livia; Has-Schön, Elizabeta; Heffer, Marija
2014-06-01
To evaluate in a rat animal model whether ovariectomy, high fat diet (HFD), and physical activity in the form of running affect leptin receptor (Ob-R) distribution in the brain and white fat tissue compared to sham (Sh) surgery, standard diet (StD), and sedentary conditions. The study included 48 female laboratory Wistar rats (4 weeks old). Following eight weeks of feeding with standard or HFD, rats were subjected to either OVX or Sh surgery. After surgery, all animals continued StD or HFD for the next 10 weeks. During these 10 weeks, ovariectomy and Sh groups were subjected to physical activity or sedentary conditions. Free-floating immunohistochemistry and Western blot methods were carried out to detect Ob-R in the brain and adipose tissue. StD-ovariectomy-sedentary group had a greater number of Ob-R positive neurons in lateral hypothalamic nuclei than StD-Sh-sedentary group. There was no difference in Ob-R positive neurons in arcuatus nuclei between all groups. Ob-R distribution in the barrel cortex was higher in HFD group than in StD group. Ob-R presence in perirenal and subcutaneous fat was decreased in StD-ovariectomy group. HFD and ovariectomy increased Ob-R distribution in lateral hypothalamic nuclei, but there was no effect on arcuatus nuclei. Our results are first to suggest that HFD, ovariectomy, and physical activity affect Ob-R distribution in the barrel cortex, which might be correlated with the role of Ob-R in election of food in rats.
NASA Astrophysics Data System (ADS)
Abdikan, S.; Sekertekin, A.; Ustunern, M.; Balik Sanli, F.; Nasirzadehdizaji, R.
2018-04-01
Temporal monitoring of crop types is essential for the sustainable management of agricultural activities on both national and global levels. As a practical and efficient tool, remote sensing is widely used in such applications. In this study, Sentinel-1 Synthetic Aperture Radar (SAR) imagery was utilized to investigate the performance of the sensor backscatter image on crop monitoring. Multi-temporal C-band VV and VH polarized SAR images were acquired simultaneously by in-situ measurements which was conducted at Konya basin, central Anatolia Turkey. During the measurements, plant height of maize plant was collected and relationship between backscatter values and plant height was analysed. The maize growth development was described under Biologische Bundesanstalt, bundessortenamt und CHemische industrie (BBCH). Under BBCH stages, the test site was classified as leaf development, stem elongation, heading and flowering in general. The correlation coefficient values indicated high correlation for both polarimetry during the early stages of the plant, while late stages indicated lower values in both polarimetry. As a last step, multi-temporal coverage of crop fields was analysed to map seasonal land use. To this aim, object based image classification was applied following image segmentation. About 80 % accuracies of land use maps were created in this experiment. As preliminary results, it is concluded that Sentinel-1 data provides beneficial information about plant growth. Dual-polarized Sentinel-1 data has high potential for multi-temporal analyses for agriculture monitoring and reliable mapping.
Martineau, Xavier; Abed, Élie; Martel-Pelletier, Johanne; Pelletier, Jean-Pierre; Lajeunesse, Daniel
2017-01-01
Objective Clinical and in vitro studies suggest that subchondral bone sclerosis due to abnormal osteoblasts (Ob) is involved in the progression and/or onset of osteoarthritis (OA). Human Ob isolated from sclerotic subchondral OA bone tissue show an altered phenotype, a decreased canonical Wnt/β-catenin signaling pathway (cWnt), and a reduced mineralization in vitro. In addition to the cWnt pathway, at least two non-canonical signaling pathways, the Wnt/PKC and Wnt/PCP pathway have been described. However, there are no reports of either pathway in OA Ob. Here, we studied the two non-canonical pathways in OA Ob and if they influence their phenotype. Methods Human primary subchondral Ob were isolated from the subchondral bone plate of tibial plateaus of OA patients undergoing total knee arthroplasty, or of normal individuals at autopsy. The expression of genes involved in non-canonical Wnt signaling was evaluated by qRT-PCR and their protein production by Western blot analysis. Alkaline phosphatase activity and osteocalcin secretion (OC) were determined with substrate hydrolysis and EIA, respectively. Mineralization levels were evaluated with Alizarin Red Staining, Wnt/PKC and Wnt/PCP pathways by target gene expression and their respective activity using the NFAT and AP-1 luciferase reporter assays. Results OA Ob showed an altered phenotype as illustrated by an increased alkaline phosphatase activity and osteocalcin release compared to normal Ob. The expression of the non-canonical Wnt5a ligand was increased in OA Ob compared to normal. Whereas, the expression of LGR5 was significantly increased in OA Ob compared to normal Ob, the expression of LGR4 was similar. Wnt5a directly stimulated the expression and production of LGR5, contrasting, Wnt5a did not stimulate the expression of LGR4. Wnt5a also stimulated the phosphorylation of both JNK and PKC, as well as the activity of both NFAT and AP-1 transcription factors. The inhibition of Wnt5a expression partially corrects the abnormal mineralization, OC secretion and ALPase activity of OA Ob. Conclusion These data indicate that the alteration of Wnt5a, a non-canonical Wnt signaling activator, is implicated in the modified signalisation and phenotype observed in OA Ob. PMID:28777797
Schönke, Milena; Björnholm, Marie; Chibalin, Alexander V; Zierath, Juleen R; Deshmukh, Atul S
2018-03-01
Skeletal muscle insulin resistance, an early metabolic defect in the pathogenesis of type 2 diabetes (T2D), may be a cause or consequence of altered protein expression profiles. Proteomics technology offers enormous promise to investigate molecular mechanisms underlying pathologies, however, the analysis of skeletal muscle is challenging. Using state-of-the-art multienzyme digestion and filter-aided sample preparation (MED-FASP) and a mass spectrometry (MS)-based workflow, we performed a global proteomics analysis of skeletal muscle from leptin-deficient, obese, insulin resistant (ob/ob) and lean mice in mere two fractions in a short time (8 h per sample). We identified more than 6000 proteins with 118 proteins differentially regulated in obesity. This included protein kinases, phosphatases, and secreted and fiber type associated proteins. Enzymes involved in lipid metabolism in skeletal muscle from ob/ob mice were increased, providing evidence against reduced fatty acid oxidation in lipid-induced insulin resistance. Mitochondrial and peroxisomal proteins, as well as components of pyruvate and lactate metabolism, were increased. Finally, the skeletal muscle proteome from ob/ob mice displayed a shift toward the "slow fiber type." This detailed characterization of an obese rodent model of T2D demonstrates an efficient workflow for skeletal muscle proteomics, which may easily be adapted to other complex tissues. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Clavijo, Gabriel; Williams, Trevor; Muñoz, Delia; Caballero, Primitivo; López-Ferber, Miguel
2010-01-01
An insect nucleopolyhedrovirus naturally survives as a mixture of at least nine genotypes. Infection by multiple genotypes results in the production of virus occlusion bodies (OBs) with greater pathogenicity than those of any genotype alone. We tested the hypothesis that each OB contains a genotypically diverse population of virions. Few insects died following inoculation with an experimental two-genotype mixture at a dose of one OB per insect, but a high proportion of multiple infections were observed (50%), which differed significantly from the frequencies predicted by a non-associated transmission model in which genotypes are segregated into distinct OBs. By contrast, insects that consumed multiple OBs experienced higher mortality and infection frequencies did not differ significantly from those of the non-associated model. Inoculation with genotypically complex wild-type OBs indicated that genotypes tend to be transmitted in association, rather than as independent entities, irrespective of dose. To examine the hypothesis that virions may themselves be genotypically heterogeneous, cell culture plaques derived from individual virions were analysed to reveal that one-third of virions was of mixed genotype, irrespective of the genotypic composition of the OBs. We conclude that co-occlusion of genotypically distinct virions in each OB is an adaptive mechanism that favours the maintenance of virus diversity during insect-to-insect transmission. PMID:19939845
Adipose Deficiency of Nrf2 in ob/ob Mice Results in Severe Metabolic Syndrome
Xue, Peng; Hou, Yongyong; Chen, Yanyan; Yang, Bei; Fu, Jingqi; Zheng, Hongzhi; Yarborough, Kathy; Woods, Courtney G.; Liu, Dianxin; Yamamoto, Masayuki; Zhang, Qiang; Andersen, Melvin E.; Pi, Jingbo
2013-01-01
Nuclear factor E2–related factor 2 (Nrf2) is a transcription factor that functions as a master regulator of the cellular adaptive response to oxidative stress. Our previous studies showed that Nrf2 plays a critical role in adipogenesis by regulating expression of CCAAT/enhancer-binding protein β and peroxisome proliferator–activated receptor γ. To determine the role of Nrf2 in the development of obesity and associated metabolic disorders, the incidence of metabolic syndrome was assessed in whole-body or adipocyte-specific Nrf2-knockout mice on a leptin-deficient ob/ob background, a model with an extremely positive energy balance. On the ob/ob background, ablation of Nrf2, globally or specifically in adipocytes, led to reduced white adipose tissue (WAT) mass, but resulted in an even more severe metabolic syndrome with aggravated insulin resistance, hyperglycemia, and hypertriglyceridemia. Compared with wild-type mice, WAT of ob/ob mice expressed substantially higher levels of many genes related to antioxidant response, inflammation, adipogenesis, lipogenesis, glucose uptake, and lipid transport. Absence of Nrf2 in WAT resulted in reduced expression of most of these factors at mRNA or protein levels. Our findings support a novel role for Nrf2 in regulating adipose development and function, by which Nrf2 controls the capacity of WAT expansion and insulin sensitivity and maintains glucose and lipid homeostasis. PMID:23238296
Watanabe, E; Lee, J S; Kawakubo, K
2011-07-01
Maternal employment has been shown to be associated with childhood overweight and obesity (Ow/Ob), but the presence of family members who care for children in place of the mothers might influence children's Ow/Ob and lifestyles. The influence of maternal employment on children's Ow/Ob should be examined together with the presence of caregivers such as grandparents. The effects of maternal employment and the presence of grandparents on lifestyles and Ow/Ob in Japanese pre-school children were investigated. Cross-sectional study on 2114 children aged 3-6 years who attended all childcare facilities in a city and primary caregivers was conducted. Children's weight and height, family environments (family members, maternal employment, single parent, number of siblings and parental Ow/Ob) and lifestyles (dietary, physical activity and sleeping habits) were surveyed using a self-administered questionnaire. Ow/Ob was defined by the International Obesity Task Force cut-offs. The eligible participants were 1765 children. The prevalence of Ow/Ob was 8.4% in boys and 9.9% in girls. Maternal employment was associated positively with irregular mealtimes, unfixed snacking times, bedtime after 10 p.m. and nighttime sleep duration of less than 10 h, whereas three-generation families were associated negatively with irregular mealtimes after adjustment for children's characteristics and family environments. Irregular mealtimes (OR (95% CI); 2.03 (1.36, 3.06)) and nighttime sleep duration of less than 10 h (1.96 (1.28, 3.01)) were associated with increased risks of being Ow/Ob. Both maternal employment and three-generation families were significantly associated with children's Ow/Ob. However, three-generation families maintained a significant association (1.59 (1.08, 2.35)) after adjustment for maternal employment. These study results suggest that the grandparents who care for pre-school children in place of mothers are more likely to contribute to childhood Ow/Ob than maternal employment. The family-focused lifestyle strategies to prevent childhood Ow/Ob must include grandparents who care for children.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Wattanachanya, Lalita, E-mail: lalita_md@yahoo.com; Division of Endocrinology and Metabolism, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok; Wang, Liping, E-mail: lipingwang05@yahoo.com
G protein-coupled receptor (GPCR) signaling in osteoblasts (OBs) is an important regulator of bone formation. We previously described a mouse model expressing Rs1, an engineered constitutively active G{sub s}-coupled GPCR, under the control of the 2.3 kb Col I promoter. These mice showed a dramatic age-dependent increase in trabecular bone of femurs. Here, we further evaluated the effects of enhanced G{sub s} signaling in OBs on intramembranous bone formation by examining calvariae of 1- and 9-week-old Col1(2.3)/Rs1 mice and characterized the in vivo gene expression specifically occurring in osteoblasts with activated G{sub s} G protein-coupled receptor signaling, at the cellularmore » level rather than in a whole bone. Rs1 calvariae displayed a dramatic increase in bone volume with partial loss of cortical structure. By immunohistochemistry, Osterix was detected in cells throughout the inter-trabecular space while Osteocalcin was expressed predominantly in cells along bone surfaces, suggesting the role of paracrine mediators secreted from OBs driven by 2.3 kb Col I promoter could influence early OB commitment, differentiation, and/or proliferation. Gene expression analysis of calvarial OBs revealed that genes affected by Rs1 signaling include those encoding proteins important for cell differentiation, cytokines and growth factors, angiogenesis, coagulation, and energy metabolism. The set of G{sub s}-GPCRs and other GPCRs that may contribute to the observed skeletal phenotype and candidate paracrine mediators of the effect of G{sub s} signaling in OBs were also determined. Our results identify novel detailed in vivo cellular changes of the anabolic response of the skeleton to G{sub s} signaling in mature OBs. - Highlights: • OB expression of an engineered G{sub s}-coupled receptor dramatically increases bone mass. • We investigated the changes in gene expression in vivo in enhanced OB G{sub s} signaling. • Genes in cell cycle and transcription were increased in enhanced OB G{sub s} signaling. • GPCRs and paracrine mediators of the effect of G{sub s} signaling in OBs were determined.« less
Initial Validation and Results of Geoscience Laser Altimeter System Optical Properties Retrievals
NASA Technical Reports Server (NTRS)
Hlavka, Dennis L.; Hart, W. D.; Pal, S. P.; McGill, M.; Spinhirne, J. D.
2004-01-01
Verification of Geoscience Laser Altimeter System (GLAS) optical retrievals is . problematic in that passage over ground sites is both instantaneous and sparse plus space-borne passive sensors such as MODIS are too frequently out of sync with the GLAS position. In October 2003, the GLAS Validation Experiment was executed from NASA Dryden Research Center, California to greatly increase validation possibilities. The high-altitude NASA ER-2 aircraft and onboard instrumentation of Cloud Physics Lidar (CPL), MODIS Airborne Simulator (MAS), and/or MODIS/ASTER Airborne Simulator (MASTER) under-flew seven orbit tracks of GLAS for cirrus, smoke, and urban pollution optical properties inter-comparisons. These highly calibrated suite of instruments are the best data set yet to validate GLAS atmospheric parameters. In this presentation, we will focus on the inter-comparison with GLAS and CPL and draw preliminary conclusions about the accuracies of the GLAS 532nm retrievals of optical depth, extinction, backscatter cross section, and calculated extinction-to-backscatter ratio. Comparisons to an AERONET/MPL ground-based site at Monterey, California will be attempted. Examples of GLAS operational optical data products will be shown.
Multi-Sensor Approach for Assessing the Taiga-Tundra Boundary
NASA Technical Reports Server (NTRS)
Ranson, K. J.; Sun, G.; Kharuk, V. I.; Kovacs, K.
2003-01-01
Monitoring the dynamics of the tundra-taiga boundary is critical for our understanding of the causes and consequences of the changes in this area. Because of its inaccessibility, remote sensing data will play an important role. In this study we examined the use of several remote sensing techniques for identifying the existing tundra-taiga ecotone. These include Landsat, MISR and RADARSAT data. High-resolution IKONOS images were used for local ground truth. It was found that on Landsat ETM+ summer images, reflectance from tundra and taiga at band 4 (NIR) is similar, but different at other bands such as red, and MIR bands. When the incidence angle is small, C-band HH-pol backscattering coefficients from both tundra and taiga are relatively high. The backscattering from tundra targets decreases faster than taiga targets when the incidence angle increases, because the tundra targets look smoother than taiga. Because of the shading effect of the vegetation, the MISR data, both multi-spectral data at nadir looking and multi-angle data at red and NIR bands, clearly show the transition zone.
NASA Technical Reports Server (NTRS)
Bhartia, Pawan K.; Ziemke, Jerry; Chandra, Sushil; Joiner, Joanna; Vassilkov, Alexandra; Taylor, Steven; Yang, Kai; Ahn, Chang-Woo
2004-01-01
The Cloud Slicing technique has emerged as a powerful tool for the study of ozone in the upper troposphere. In this technique one looks at the variation with cloud height of the above-cloud column ozone derived from the backscattered ultraviolet instruments, such as TOMS, to determine the ozone mixing ratio. For this technique to work properly one needs an instrument with relatively good horizontal resolution with very good signal to noise in measuring above-cloud column ozone. In addition, one needs the (radiatively) effective cloud pressure rather than the cloud-top pressure, for the ultraviolet photons received by a satellite instrument are scattered from inside the cloud rather than from the top. For this study we use data from the OMI sensor, which was recently launched on the EOS Aura satellite. OMI is a W-Visible backscattering instrument with a nadir pixel size of 13 x 24 km. The effective cloud pressure is derived from a new algorithm based on Rotational Raman Scattering and O2-O2, absorption in the 340-400 nm band of OMI.
NASA Astrophysics Data System (ADS)
Jarvis, Jan; Haertelt, Marko; Hugger, Stefan; Butschek, Lorenz; Fuchs, Frank; Ostendorf, Ralf; Wagner, Joachim; Beyerer, Juergen
2017-04-01
In this work we present data analysis algorithms for detection of hazardous substances in hyperspectral observations acquired using active mid-infrared (MIR) backscattering spectroscopy. We present a novel background extraction algorithm based on the adaptive target generation process proposed by Ren and Chang called the adaptive background generation process (ABGP) that generates a robust and physically meaningful set of background spectra for operation of the well-known adaptive matched subspace detection (AMSD) algorithm. It is shown that the resulting AMSD-ABGP detection algorithm competes well with other widely used detection algorithms. The method is demonstrated in measurement data obtained by two fundamentally different active MIR hyperspectral data acquisition devices. A hyperspectral image sensor applicable in static scenes takes a wavelength sequential approach to hyperspectral data acquisition, whereas a rapid wavelength-scanning single-element detector variant of the same principle uses spatial scanning to generate the hyperspectral observation. It is shown that the measurement timescale of the latter is sufficient for the application of the data analysis algorithms even in dynamic scenarios.
Schaber, Gerald G.; McCauley, John F.; Breed, Carol S.; Olhoeft, Gary R.
1986-01-01
It is found that the Shuttle Imaging Radar A (SIR-A) signal penetration and subsurface backscatter within the upper meter or so of the sediment blanket in the Eastern Sahara of southern Egypt and northern Sudan are enhanced both by radar sensor parameters and by the physical and chemical characteristics of eolian and alluvial materials. The near-surface stratigraphy, the electrical properties of materials, and the types of radar interfaces found to be responsible for different classes of SIR-A tonal response are summarized. The dominant factors related to efficient microwave signal penetration into the sediment blanket include 1) favorable distribution of particle sizes, 2) extremely low moisture content and 3) reduced geometric scattering at the SIR-A frequency (1. 3 GHz). The depth of signal penetration that results in a recorded backscatter, called radar imaging depth, was documented in the field to be a maximum of 1. 5 m, or 0. 25 times the calculated skin depth, for the sediment blanket. The radar imaging depth is estimated to be between 2 and 3 m for active sand dune materials.
NASA Astrophysics Data System (ADS)
Hu, Chuanmin; Lee, Zhongping; Muller-Karger, Frank E.; Carder, Kendall L.
2003-05-01
A spectra-matching optimization algorithm, designed for hyperspectral sensors, has been implemented to process SeaWiFS-derived multi-spectral water-leaving radiance data. The algorithm has been tested over Southwest Florida coastal waters. The total spectral absorption and backscattering coefficients can be well partitioned with the inversion algorithm, resulting in RMS errors generally less than 5% in the modeled spectra. For extremely turbid waters that come from either river runoff or sediment resuspension, the RMS error is in the range of 5-15%. The bio-optical parameters derived in this optically complex environment agree well with those obtained in situ. Further, the ability to separate backscattering (a proxy for turbidity) from the satellite signal makes it possible to trace water movement patterns, as indicated by the total absorption imagery. The derived patterns agree with those from concurrent surface drifters. For waters where CDOM overwhelmingly dominates the optical signal, however, the procedure tends to regard CDOM as the sole source of absorption, implying the need for better atmospheric correction and for adjustment of some model coefficients for this particular region.
Ambulatory Blood Pressure Monitoring in Lean, Obese and Diabetic Children and Adolescents
Shikha, Deep; Singla, Montish; Walia, Rachna; Potter, Natia; Umpaichitra, Vatcharapan; Mercado, Arlene; Winer, Nathaniel
2015-01-01
Aim To determine if children and adolescents who have obesity (Ob) or type 2 diabetes (T2DM) of relatively short duration have impaired cardiovascular function compared with lean subjects using 24-hour ambulatory blood pressure as a surrogate measure of evaluation. Methods We enrolled 100 African-Caribbean subjects (45 males/55 females), mean ages 14.4-15.2 years (range 11.8-18.5 years) and Tanner stage 4.2-4.8. Mean BMI for the Ob (n = 40), T2DM (n = 39) and lean (n = 21) groups were 40.3, 34.2 and 20.8, respectively (p < 0.01, Ob and T2DM vs. lean). Mean hemoglobin A1c in lean and Ob was 5.4 and 5.5% compared to 8.8% in T2DM (p < 0.001, T2DM vs. lean and Ob). Ambulatory blood pressure was recorded every 20 min over 24 h using Spacelabs 70207. Results Mean 24-hour, daytime and nighttime systolic blood pressure was significantly higher in Ob and T2DM compared with lean subjects (mean 24-hour 117 and 120 vs. 109 mm Hg; daytime 121 and 123 vs. 113 mm Hg; and nighttime 109 and 115 vs. 101 mm Hg; p < 0.01 for all time periods). The nocturnal systolic dip in Ob and T2DM did not differ from that of lean, whereas nocturnal diastolic dip decreased significantly in Ob and T2DM compared to lean (11.5 and 10.4 vs. 20.6 mm Hg; p < 0.01). Mean pulse pressure was significantly increased in the Ob and T2DM groups compared to lean subjects (51 and 54 vs. 45 mm Hg; p < 0.01). Conclusion Adolescent Ob and T2DM groups share adverse risk factors, which may be harbingers of adult cardiovascular events. PMID:26195970
Kaiyala, Karl J; Ogimoto, Kayoko; Nelson, Jarrell T; Schwartz, Michael W; Morton, Gregory J
2015-01-01
Survival of free-living animals depends on the ability to maintain core body temperature in the face of rapid and dramatic changes in their thermal environment. If food intake is not adjusted to meet the changing energy demands associated with changes of ambient temperature, a serious challenge to body energy stores can occur. To more fully understand the coupling of thermoregulation to energy homeostasis in normal animals and to investigate the role of the adipose hormone leptin to this process, comprehensive measures of energy homeostasis and core temperature were obtained in leptin-deficient ob/ob mice and their wild-type (WT) littermate controls when housed under cool (14°C), usual (22°C) or ∼ thermoneutral (30°C) conditions. Our findings extend previous evidence that WT mice robustly defend normothermia in response to either a lowering (14°C) or an increase (30°C) of ambient temperature without changes in body weight or body composition. In contrast, leptin-deficient, ob/ob mice fail to defend normothermia at ambient temperatures lower than thermoneutrality and exhibit marked losses of both body fat and lean mass when exposed to cooler environments (14°C). Our findings further demonstrate a strong inverse relationship between ambient temperature and energy expenditure in WT mice, a relationship that is preserved in ob/ob mice. However, thermal conductance analysis indicates defective heat retention in ob/ob mice, irrespective of temperature. While a negative relationship between ambient temperature and energy intake also exists in WT mice, this relationship is disrupted in ob/ob mice. Thus, to meet the thermoregulatory demands of different ambient temperatures, leptin signaling is required for adaptive changes in both energy intake and thermal conductance. A better understanding of the mechanisms coupling thermoregulation to energy homeostasis may lead to the development of new approaches for the treatment of obesity.
Kaiyala, Karl J.; Ogimoto, Kayoko; Nelson, Jarrell T.; Schwartz, Michael W.; Morton, Gregory J.
2015-01-01
Survival of free-living animals depends on the ability to maintain core body temperature in the face of rapid and dramatic changes in their thermal environment. If food intake is not adjusted to meet the changing energy demands associated with changes of ambient temperature, a serious challenge to body energy stores can occur. To more fully understand the coupling of thermoregulation to energy homeostasis in normal animals and to investigate the role of the adipose hormone leptin to this process, comprehensive measures of energy homeostasis and core temperature were obtained in leptin-deficient ob/ob mice and their wild-type (WT) littermate controls when housed under cool (14°C), usual (22°C) or ∼ thermoneutral (30°C) conditions. Our findings extend previous evidence that WT mice robustly defend normothermia in response to either a lowering (14°C) or an increase (30°C) of ambient temperature without changes in body weight or body composition. In contrast, leptin-deficient, ob/ob mice fail to defend normothermia at ambient temperatures lower than thermoneutrality and exhibit marked losses of both body fat and lean mass when exposed to cooler environments (14°C). Our findings further demonstrate a strong inverse relationship between ambient temperature and energy expenditure in WT mice, a relationship that is preserved in ob/ob mice. However, thermal conductance analysis indicates defective heat retention in ob/ob mice, irrespective of temperature. While a negative relationship between ambient temperature and energy intake also exists in WT mice, this relationship is disrupted in ob/ob mice. Thus, to meet the thermoregulatory demands of different ambient temperatures, leptin signaling is required for adaptive changes in both energy intake and thermal conductance. A better understanding of the mechanisms coupling thermoregulation to energy homeostasis may lead to the development of new approaches for the treatment of obesity. PMID:25756181
Obstetrician–Gynecologists’ Objections to and Willingness to Help Patients Obtain an Abortion
Harris, Lisa H; Cooper, Alexandra; Rasinski, Kenneth A; Curlin, Farr A; Lyerly, Anne Drapkin
2014-01-01
Objective To describe obstetrician–gynecologists’ (ob-gyns) views and willingness to help women seeking abortion in a variety of clinical scenarios. Methods We conducted a mailed survey of 1,800 U.S. ob-gyns. We presented seven scenarios in which patients sought abortion. For each, respondents indicated if they morally objected to abortion and if they would help patients obtain an abortion. We analyzed predictors of objection and assistance. Results The response rate was 66%. Objection to abortion ranged from 16% (cardiopulmonary disease) to 82% (sex selection); willingness to assist ranged from 64% (sex selection) to 93% (cardiopulmonary disease). Excluding sex selection, objection was less likely among ob-gyns who were female (odds ratio [OR] 0.5, 95% confidence interval [CI] 0.4–0.8), urban (OR 0.3, CI 0.1–0.7), or Jewish (OR 0.3, CI 0.1–0.7) compared to male, rural, or unaffiliated ob-gyns. Objection was more likely among ob-gyns from the South (OR 1.9, CI 1.2–3.0) or Midwest (OR 1.9, CI 1.2–3.1), and among Catholic, evangelical Protestant, or Muslim ob-gyns, or those for whom religion was most important, compared to reference. Among ob-gyns who objected to abortion in a given case, approximately two-thirds nevertheless help patients obtain an abortion. Excluding sex selection, assistance despite objection was more likely among female (OR 1.8, CI 1.1–2.9) and US-born ob-gyns (OR 2.2, CI 1.1–4.7), and less likely among Southern ob-gyns (OR 0.3, CI 0.2–0.6), or those for whom religion was most important (OR 0.3, CI 0.1–0.7). Conclusions Most ob-gyns help patients obtain an abortion even when they morally object to abortion in that case. Willingness to assist varies by clinical context and physician characteristics. PMID:21934455
Gender and sexual health in clinical practice in Lebanon
El-Kak, F.; Jurdi, R.; Kaddour, A.; Zurayk, H.
2006-01-01
This study examines the readiness of obstetricians/gynecologists (Ob/Gyns) in Lebanon to provide sexual consultation, their degree of comfort when discussing issues of sexual health, and their attitudes regarding assessment, treatment, and referral. Data on these concepts were collected through face-to-face interviews with 286 randomly selected Ob/Gyns. Most Ob/Gyns reported feeling comfortable discussing sexuality during consultations, which they attributed much more to professional experience than to training. Most Ob/Gyns reported giving proper time for management of sexual health issues and follow-up, as these issues are brought up frequently by their women clients. However, results suggest that only one-third (31%) of Lebanese Ob/Gyns nearly always take the initiative in asking patients about their sexual health. Moreover, almost 45% of participating Ob/Gyns did not recognize a strong relationship between reproductive health and sexual functioning. Gender was not found to be an important predictor for any of the indicators measured in the present study. Ob/Gyns in Lebanon are significant consultants on various sexual issues, and they need better postgraduate training, continuing medical education, and access to medical congress resources on the topic of sexuality and its relationship to reproductive health. PMID:15548405
A CATALOG OF NEW SPECTROSCOPICALLY CONFIRMED MASSIVE OB STARS IN CARINA
DOE Office of Scientific and Technical Information (OSTI.GOV)
Alexander, Michael J.; Hanes, Richard J.; McSwain, M. Virginia
2016-12-01
The Carina star-forming region is one of the largest in the Galaxy, and its massive star population is still being unveiled. The large number of stars combined with high, and highly variable, interstellar extinction makes it inherently difficult to find OB stars in this type of young region. We present the results of a spectroscopic campaign to study the massive star population of the Carina Nebula, with the primary goal to confirm or reject previously identified Carina OB star candidates. A total of 141 known O- and B-type stars and 94 candidates were observed, of which 73 candidates had highmore » enough signal-to-noise ratio to classify. We find 23 new OB stars within the Carina Nebula, a 32% confirmation rate. One of the new OB stars has blended spectra and is suspected to be a double-lined spectroscopic binary (SB2). We also reclassify the spectral types of the known OB stars and discover nine new SB2s among this population. Finally, we discuss the spatial distribution of these new OB stars relative to known structures in the Carina Nebula.« less
Rabi cropped area forecasting of parts of Banaskatha District,Gujarat using MRS RISAT-1 SAR data
NASA Astrophysics Data System (ADS)
Parekh, R. A.; Mehta, R. L.; Vyas, A.
2016-10-01
Radar sensors can be used for large-scale vegetation mapping and monitoring using backscatter coefficients in different polarisations and wavelength bands. Due to cloud and haze interference, optical images are not always available at all phonological stages important for crop discrimination. Moreover, in cloud prone areas, exclusively SAR approach would provide operational solution. This paper presents the results of classifying the cropped and non cropped areas using multi-temporal SAR images. Dual polarised C- band RISAT MRS (Medium Resolution ScanSAR mode) data were acquired on 9thDec. 2012, 28thJan. 2013 and 22nd Feb. 2013 at 18m spatial resolution. Intensity images of two polarisations (HH, HV) were extracted and converted into backscattering coefficient images. Cross polarisation ratio (CPR) images and Radar fractional vegetation density index (RFDI) were created from the temporal data and integrated with the multi-temporal images. Signatures of cropped and un-cropped areas were used for maximum likelihood supervised classification. Separability in cropped and umcropped classes using different polarisation combinations and classification accuracy analysis was carried out. FCC (False Color Composite) prepared using best three SAR polarisations in the data set was compared with LISS-III (Linear Imaging Self-Scanning System-III) image. The acreage under rabi crops was estimated. The methodology developed was for rabi cropped area, due to availability of SAR data of rabi season. Though, the approach is more relevant for acreage estimation of kharif crops when frequent cloud cover condition prevails during monsoon season and optical sensors fail to deliver good quality images.
A New Study of Sea Spray Optical Properties from Multi-Sensor Spaceborne Observations
NASA Technical Reports Server (NTRS)
Dawson, K. W.; Meskhidze, N.; Josset, D.; Gasso, S.
2014-01-01
Retrievals of aerosol optical depth (AOD) from the Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) satellite sensor require the assumption of an extinction-to-backscatter ratio, also known as the lidar ratio. This paper evaluates a new method to calculate lidar ratio of sea spray aerosol using two independent sources: the AOD from Synergized Optical Depth of Aerosols (SODA) and the integrated attenuated backscatter from CALIOP. The method applied in this study allows particulate lidar ratio to be calculated for individual CALIOP retrievals of single aerosol layer columns over the ocean. Analyses are carried out using CALIOP level 2, 5km sea spray aerosol layer products and collocated SODA nighttime data from December 2007 to December 2009. The global mean lidar ratio for sea spray aerosols was found to be 26 sr, roughly 30 higher than the one prescribed by CALIOP. Data analysis also showed considerable spatiotemporal variability in calculated lidar ratio over different parts of the remote oceans. The calculated aerosol lidar ratios are shown to be inversely related to the mean ocean surface wind speed: increase in ocean surface wind speed (U10) from 0 to 15 ms-1 reduces the mean lidar ratios for sea spray particles from 32 sr (for 0 U10 4 ms-1) to 22 sr (for U10 15 ms-1). Such changes in the lidar ratio are expected to have a corresponding effect on sea spray AOD. The outcomes of this study are relevant for future improvements of the SODA and CALIOP operational product and could lead to more accurate retrievals of sea spray AOD.
Remote sensing of particle backscattering in Chesapeake Bay: a 6-year SeaWiFS retrospective view
Zawada, D.G.; Hu, C.; Clayton, T.; Chen, Z.; Brock, J.C.; Muller-Karger, F. E.
2007-01-01
Traditional field techniques to monitor water quality in large estuaries, such as boat-based surveys and autonomous moored sensors, generally provide limited spatial coverage. Satellite imagery potentially can be used to address both of these limitations. Here, we show that satellite-based observations are useful for inferring total-suspended-solids (TSS) concentrations in estuarine areas. A spectra-matching optimization algorithm was used to estimate the particle backscattering coefficient at 400 nm, bbp(400), in Chesapeake Bay from Sea-viewing Wide-Field-of-view Sensor (SeaWiFS) satellite imagery. These estimated values of bbp(400) were compared to in situ measurements of TSS for the study period of September 1997–December 2003. Contemporaneous SeaWiFS bbp(400) values and TSS concentrations were positively correlated (N = 340, r2 = 0.4, P bp(400) values served as a reasonable first-order approximation for synoptically mapping TSS. Overall, large-scale patterns of SeaWiFS bbp(400) appeared to be consistent with expectations based on field observations and historical reports of TSS. Monthly averages indicated that SeaWiFS bbp(400) was typically largest in winter (>0.049 m−1, November–February) and smallest in summer (−1, June–August), regardless of the amount of riverine discharge to the bay. The study period also included Hurricanes Floyd and Isabel, which caused large-scale turbidity events and changes in the water quality of the bay. These results demonstrate that this technique can provide frequent synoptic assessments of suspended solids concentrations in Chesapeake Bay and other coastal regions.
NASA Astrophysics Data System (ADS)
Tian, Chunlei; Yin, Yawei; Wu, Jian; Lin, Jintong
2008-11-01
The interworking network of Generalized Multi-Protocol Label Switching (GMPLS) and Optical Burst Switching (OBS) is attractive network architecture for the future IP/DWDM network nowadays. In this paper, OSPF-TE extensions for multi-domain Optical Burst Switching networks connected by GMPLS controlled WDM network are proposed, the corresponding experimental results such as the advertising latency are also presented by using an OBS network testbed. The experimental results show that it works effectively on the OBS/GMPLS networks.
Searching for self-enrichment in Cygnus OB2
NASA Astrophysics Data System (ADS)
Berlanas, Sara R.; Herrero, Artemio; Comerón, Fernando; Pasquali, Anna; Simón-Díaz, Sergio
2017-11-01
Cygnus OB2 is a rich and relatively close (d~1.4 kpc) OB association in our Galaxy. It represents an ideal testbed for our theories about self-enrichment processes produced by pollution of the interstellar medium by successive generations of massive stars. Comerón & Pasquali (2012, A&A, 543, A101) found a correlation between the age of young stellar groups in Cygnus OB2 and their Galactic longitude. If is associated with a chemical composition gradient, it could support these self-enrichment processes.
Meijome, Tomas E; Baughman, Jenna T; Hooker, R Adam; Cheng, Ying-Hua; Ciovacco, Wendy A; Balamohan, Sanjeev M; Srinivasan, Trishya L; Chitteti, Brahmananda R; Eleniste, Pierre P; Horowitz, Mark C; Srour, Edward F; Bruzzaniti, Angela; Fuchs, Robyn K; Kacena, Melissa A
2016-04-01
C-Mpl is the receptor for thrombopoietin (TPO), the main megakaryocyte (MK) growth factor, and c-Mpl is believed to be expressed on cells of the hematopoietic lineage. As MKs have been shown to enhance bone formation, it may be expected that mice in which c-Mpl was globally knocked out (c-Mpl(-/-) mice) would have decreased bone mass because they have fewer MKs. Instead, c-Mpl(-/-) mice have a higher bone mass than WT controls. Using c-Mpl(-/-) mice we investigated the basis for this discrepancy and discovered that c-Mpl is expressed on both osteoblasts (OBs) and osteoclasts (OCs), an unexpected finding that prompted us to examine further how c-Mpl regulates bone. Static and dynamic bone histomorphometry parameters suggest that c-Mpl deficiency results in a net gain in bone volume with increases in OBs and OCs. In vitro, a higher percentage of c-Mpl(-/-) OBs were in active phases of the cell cycle, leading to an increased number of OBs. No difference in OB differentiation was observed in vitro as examined by real-time PCR and functional assays. In co-culture systems, which allow for the interaction between OBs and OC progenitors, c-Mpl(-/-) OBs enhanced osteoclastogenesis. Two of the major signaling pathways by which OBs regulate osteoclastogenesis, MCSF/OPG/RANKL and EphrinB2-EphB2/B4, were unaffected in c-Mpl(-/-) OBs. These data provide new findings for the role of MKs and c-Mpl expression in bone and may provide insight into the homeostatic regulation of bone mass as well as bone loss diseases such as osteoporosis. © 2015 Wiley Periodicals, Inc.
Pena, R R; Pereira-Caixeta, A R; Moraes, M F D; Pereira, G S
2014-10-01
To identify an individual as familiar, rodents form a specific type of memory named social recognition memory. The olfactory bulb (OB) is an important structure for social recognition memory, while the hippocampus recruitment is still controversial. The present study was designed to elucidate the OB and the dorsal hippocampus contribution to the consolidation of social memory. For that purpose, we tested the effect of anisomycin (ANI), which one of the effects is the inhibition of protein synthesis, on the consolidation of social recognition memory. Swiss adult mice with cannulae implanted into the CA1 region of the dorsal hippocampus or into the OB were exposed to a juvenile during 5 min (training session; TR), and once again 1.5 h or 24 h later to test social short-term memory (S-STM) or social long-term memory (S-LTM), respectively. To study S-LTM consolidation, mice received intra-OB or intra-CA1 infusion of saline or ANI immediately, 3, 6 or 18 h after TR. ANI impaired S-LTM consolidation in the OB, when administered immediately or 6h after TR. In the dorsal hippocampus, ANI was amnesic only if administered 3 h after TR. Furthermore, the infusion of ANI in either OB or CA1, immediately after training, did not affect S-STM. Moreover, ANI administered into the OB did not alter the animal's performance in the buried food-finding task. Altogether, our results suggest the consolidation of S-LTM requires both OB and hippocampus participation, although in different time points. This study may help shedding light on the specific roles of the OB and dorsal hippocampus in social recognition memory. Copyright © 2014 Elsevier Inc. All rights reserved.
Lin, Hung-Yun; Yang, Sheng-Huei; Tang, Heng-Yuan; Cheng, Guei-Yun; Davis, Paul J; Grasso, Patricia
2014-07-01
The effects of leptin-related synthetic peptides [d-Leu-4]-OB3 and OB3 on energy balance and glucose homeostasis in ob/ob and db/db mice have been confirmed. The molecular basis of these effects, however, remains unclear. In the present study, we examined the ability of these peptides to activate signal transduction pathways known to be involved in transduction of the leptin signal. In a specific and concentration-dependent manner, [d-Leu-4]-OB3 induced phosphorylation of ERK1/2, PI-3K, Ser-727 STAT3, and Tyr-705 of STAT3. OB3 also induced activation of STAT3 via phosphorylation of ERK1/2, STAT3 Ser-727, STAT3 Tyr-705 and PI-3K p85, but to a lesser degree. Using PD98059 and LY294002, specific inhibitors of MEK and PI-3K, respectively, we were able to identify the signal transduction pathways involved in peptide-induced STAT3 activation. [d-Leu-4]-OB3 induced serine phosphorylation of STAT3 primarily through activation of ERK1/2. Tyrosine phosphorylation of STAT3, however, was induced primarily through activation of PI-3K. Our data suggest that in db/db mice, [d-Leu-4]-OB3 binding to short isoforms of the leptin receptor induces intracellular signaling cascades which do not require OB-Rb activation. These signals may ultimately result in peptide effects on transcriptional and translational events associated with energy balance and glycemic regulation. In summary, we have shown for the first time that, similar to leptin, bioactive leptin-related synthetic peptide analogs activate STAT3 via phosphorylation of serine and tyrosine residues by multiple signal transduction pathways. Copyright © 2014 Elsevier Inc. All rights reserved.
Mizuno, Márcia Sanae; Crisma, Amanda Rabello; Borelli, Primavera; Castelucci, Patricia
2012-01-01
AIM: To examine whether the ob/ob mouse model of obesity is accompanied by enteric nervous system abnormalities such as altered motility. METHODS: The study examined the distribution of the P2X2 receptor (P2X2R) in myenteric neurons of female ob/ob mice. Specifically, we used immunohistochemistry to analyze the co-expression of the P2X2R with neuronal nitric oxide synthase (nNOS), choline acetyltransferase (ChAT), and calretinin (CalR) in neurons of the small intestine myenteric plexus in ob/ob and control female mice. In these sections, we used scanning confocal microscopy to analyze the co-localization of these markers as well as the neuronal density (cm2) and area profile (μm²) of P2X2R-positive neurons. In addition, enteric neurons were labeled using the nicotinamide adenine dinucleotide (NADH) diaphorase method and analyzed with light microscopy as an alternate means by which to analyze neuronal density and area. RESULTS: In the present study, we observed a 29.6% increase in the body weight of the ob/ob animals (OG) compared to the control group (CG). In addition, the average small intestine area was increased by approximately 29.6% in the OG compared to the CG. Immunoreactivity (IR) for the P2X2R, nNOS, ChAT and CalR was detectable in the myenteric plexus, as well as in the smooth muscle, in both groups. This IR appeared to be mainly cytoplasmic and was also associated with the cell membrane of the myenteric plexus neurons, where it outlined the neuronal cell bodies and their processes. P2X2R-IR was observed to co-localize 100% with that for nNOS, ChAT and CalR in neurons of both groups. In the ob/ob group, however, we observed that the neuronal density (neuron/cm2) of P2X2R-IR cells was increased by 62% compared to CG, while that of NOS-IR and ChAT-IR neurons was reduced by 49% and 57%, respectively, compared to control mice. The neuronal density of CalR-IR neurons was not different between the groups. Morphometric studies further demonstrated that the cell body profile area (μm²) of nNOS-IR, ChAT-IR and CalR-IR neurons was increased by 34%, 20% and 55%, respectively, in the OG compared to controls. Staining for NADH diaphorase activity is widely used to detect alterations in the enteric nervous system; however, our qualitative examination of NADH-diaphorase positive neurons in the myenteric ganglia revealed an overall similarity between the two groups. CONCLUSION: We demonstrate increases in P2X2R expression and alterations in nNOS, ChAT and CalR IR in ileal myenteric neurons of female ob/ob mice compared to wild-type controls. PMID:23002338
Aerosol and Cloud Interaction Observed From High Spectral Resolution Lidar Data
NASA Technical Reports Server (NTRS)
Su, Wenying; Schuster, Gregory L.; Loeb, Norman G.; Rogers, Raymond R.; Ferrare, Richard A.; Hostetler, Chris A.; Hair, Johnathan W.; Obland, Michael D.
2008-01-01
Recent studies utilizing satellite retrievals have shown a strong correlation between aerosol optical depth (AOD) and cloud cover. However, these retrievals from passive sensors are subject to many limitations, including cloud adjacency (or 3D) effects, possible cloud contamination, uncertainty in the AOD retrieval. Some of these limitations do not exist in High Spectral Resolution Lidar (HSRL) observations; for instance, HSRL observations are not a ected by cloud adjacency effects, are less prone to cloud contamination, and offer accurate aerosol property measurements (backscatter coefficient, extinction coefficient, lidar ratio, backscatter Angstrom exponent,and aerosol optical depth) at a neospatial resolution (less than 100 m) in the vicinity of clouds. Hence, the HSRL provides an important dataset for studying aerosol and cloud interaction. In this study, we statistically analyze aircraft-based HSRL profiles according to their distance from the nearest cloud, assuring that all profile comparisons are subject to the same large-scale meteorological conditions. Our results indicate that AODs from HSRL are about 17% higher in the proximity of clouds (approximately 100 m) than far away from clouds (4.5 km), which is much smaller than the reported cloud 3D effect on AOD retrievals. The backscatter and extinction coefficients also systematically increase in the vicinity of clouds, which can be explained by aerosol swelling in the high relative humidity (RH) environment and/or aerosol growth through in cloud processing (albeit not conclusively). On the other hand, we do not observe a systematic trend in lidar ratio; we hypothesize that this is caused by the opposite effects of aerosol swelling and aerosol in-cloud processing on the lidar ratio. Finally, the observed backscatter Angstrom exponent (BAE) does not show a consistent trend because of the complicated relationship between BAE and RH. We demonstrate that BAE should not be used as a surrogate for Angstrom exponent, especially at high RH.
NASA Astrophysics Data System (ADS)
Molina Garcia, Victor; Sasi, Sruthy; Efremenko, Dmitry; Doicu, Adrian; Loyola, Diego
2017-04-01
In this work, the requirements for the retrieval of cloud properties in the back-scattering region are described, and their application to the measurements taken by the Earth Polychromatic Imaging Camera (EPIC) on board the Deep Space Climate Observatory (DSCOVR) is shown. Various radiative transfer models and their linearizations are implemented, and their advantages and issues are analyzed. As radiative transfer calculations in the back-scattering region are computationally time-consuming, several acceleration techniques are also studied. The radiative transfer models analyzed include the exact Discrete Ordinate method with Matrix Exponential (DOME), the Matrix Operator method with Matrix Exponential (MOME), and the approximate asymptotic and equivalent Lambertian cloud models. To reduce the computational cost of the line-by-line (LBL) calculations, the k-distribution method, the Principal Component Analysis (PCA) and a combination of the k-distribution method plus PCA are used. The linearized radiative transfer models for retrieval of cloud properties include the Linearized Discrete Ordinate method with Matrix Exponential (LDOME), the Linearized Matrix Operator method with Matrix Exponential (LMOME) and the Forward-Adjoint Discrete Ordinate method with Matrix Exponential (FADOME). These models were applied to the EPIC oxygen-A band absorption channel at 764 nm. It is shown that the approximate asymptotic and equivalent Lambertian cloud models give inaccurate results, so an offline processor for the retrieval of cloud properties in the back-scattering region requires the use of exact models such as DOME and MOME, which behave similarly. The combination of the k-distribution method plus PCA presents similar accuracy to the LBL calculations, but it is up to 360 times faster, and the relative errors for the computed radiances are less than 1.5% compared to the results when the exact phase function is used. Finally, the linearized models studied show similar behavior, with relative errors less than 1% for the radiance derivatives, but FADOME is 2 times faster than LDOME and 2.5 times faster than LMOME.
Tessmer-Tuck, Jennifer A; Rayburn, William F
2015-09-01
Obstetrician-gynecologists (OB-GYNs) are the fourth largest group of physicians and the only specialty dedicated solely to women's health care. The specialty is unique in providing 24-hour inpatient coverage, surgical care and ambulatory preventive health care. This article identifies and reviews changes in the OB-GYN workforce, including more female OB-GYNs, an increasing emphasis on work-life balance, more sub-specialization, larger group practices with more employed physicians and, finally, an emphasis on quality and performance improvement. It then describes the evolution of the OB-GYN hospitalist movement to date and the role of OB-GYN hospitalists in the future with regard to these workforce changes. Copyright © 2015 Elsevier Inc. All rights reserved.
Reduced adiposity in ob/ob mice following total body irradiation and bone marrow transplantation.
Ablamunits, Vitaly; Weisberg, Stuart P; Lemieux, Jacob E; Combs, Terry P; Klebanov, Simon
2007-06-01
The objective of this study was to assess long-term metabolic consequences of total body irradiation (TBI) and bone marrow transplantation. Severe obesity develops due to both hypertrophy and hyperplasia of adipocytes. We hypothesized that TBI would arrest adipose tissue growth and would affect insulin resistance (IR). We exposed 2-month-old female ob/ob mice to 8 Grays of TBI followed by bone marrow transplantation and tested the animals for body weight (BW) gain, body composition, blood glucose, and insulin sensitivity. Two months after TBI, irradiated mice stopped gaining BW, whereas non-treated mice continued to grow. At the age of 9.5 months, body mass of irradiated mice was 60.6 +/- 1.4 grams, which was only 61% of that in non-treated ob/ob controls (99.4 +/- 1.6 grams). Body composition measurements by DXA showed that decreased BW was primarily due to an impaired fat accumulation. This could not result from the production of leptin by bone marrow-derived adipocyte progenitors because inhibition of the obese phenotype was identical in recipients of both B6 and ob/ob bone marrow. Inability of the irradiated mice to accumulate fat was associated with hepatomegaly, lower levels of monocyte chemoattractant protein-1 expression in adipose tissue, and increased IR. Our data argue in favor of the hypothesis that inability of adipose tissue to expand may increase IR. This mouse model may be valuable for studies of late-onset radiation-induced IR in humans.
Delgado, Teresa C; Violante, Inês R; Nieto-Charques, Laura; Cerdán, Sebastián
2011-01-01
Manganese-Enhanced Magnetic Resonance Imaging (MEMRI), 1H and 13C High-Resolution-Magic Angle Spinning (HR-MAS) Spectroscopy, and genomic approaches were used to compare cerebral activation and neuronal and glial oxidative metabolism in ad libitum fed C57BL6/J leptin-deficient, genetically obese ob/ob mice. T1-weighted Magnetic Resonance Images across the hypothalamic Arcuate and the Ventromedial nuclei were acquired kinetically after manganese infusion. Neuroglial compartmentation was investigated in hypothalamic biopsies after intraperitoneal injections of [1-13C]glucose or [2-13C]acetate. Total RNA was extracted to determine the effects of leptin deficiency in the expression of representative genes coding for regulatory enzymes of hypothalamic energy pathways and glutamatergic neurotransmission. Manganese-Enhanced Magnetic Resonance Imaging revealed enhanced cerebral activation in the hypothalamic Arcuate and Ventromedial nuclei of the ob/ob mice. 13C HR-MAS analysis showed increased 13C accumulation in the hypothalamic glutamate and glutamine carbons of ob/ob mice after the administration of [1-13C]glucose, a primarily neuronal substrate. Hypothalamic expression of the genes coding for glucokinase, phosphofructokinase, pyruvate dehydrogenase, and glutamine synthase was not significantly altered while pyruvate kinase expression was slightly upregulated. In conclusion, leptin deficiency associated with obesity led to increased cerebral activation in the hypothalamic Arcuate and Ventromedial nuclei, concomitant with significant increases in neuronal oxidative metabolism and glutamatergic neurotransmission. PMID:21971349
Haro, Carmen; García-Carpintero, Sonia; Rangel-Zúñiga, Oriol A; Alcalá-Díaz, Juan F; Landa, Blanca B; Clemente, José C; Pérez-Martínez, Pablo; López-Miranda, José; Pérez-Jiménez, Francisco; Camargo, Antonio
2017-12-01
The consumption of two healthy diets (Mediterranean (MED) and low-fat (LF) diets) may restore the gut microbiome dysbiosis in obese patients depending on the degree of metabolic dysfunction. The differences in bacterial community at baseline and after 2 years of dietary intervention of 106 subjects from the CORDIOPREV study were analyzed, 33 of whom were obese patients with severe metabolic disease (5 criteria for metabolic syndrome) (MetS-OB), 32 obese patients without metabolic dysfunction (2 or less criteria for metabolic syndrome) (NonMetS-OB) and 41 non-obese subjects (NonMetS-NonOB). Our study showed a marked dysbiosis in people with severe metabolic disease (Met-OB), compared with obese people without MetS (NonMetS-OB) and non-obese people (NonMetS-NonOB). This disbiotic pattern was reversed by consumption of both MED (35% of calories as fat (22% MUFA fat, 6% PUFA fat and <10% saturated fat) or LF (<30% total fat (<10% saturated fat, 12%-14% MUFA fat and 6-8% PUFA fat) diets, whereas no significant microbiota changes were observed in NonMetS-NonOB and NonMetS-OB groups. Our results suggest that the chronic intake of two healthy dietary patterns partially restores the gut microbiome dysbiosis in obese patients with coronary heart disease, depending on the degree of metabolic dysfunction. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Sajjan, Dayanand B; Hinchigeri, Shivayogeppa B
2016-03-01
Baculoviruses are the ingenious insect pathogens. Outside the host, baculovirus occlusion bodies (OB) provide stability to occlusion-derived viruses (ODV) embedded within. The OB is an organized structure, chiefly composed of proteins namely polyhedrin, polyhedron envelope protein (PEP) and P10. Currently, the structural organization of OB is poorly understood and the role of OB proteins in conferring the stability to ODV is unknown. Here we have shown that the assembly of polyhedrin unit cells into an OB is a rapid process; the PEP forms in multiple layers; the PEP layers predominantly contribute to ODV viability. Full-grown OBs (n = 36) were found to be 4.0 ± 1.0 µm in diameter and possessed a peculiar geometry of a truncated rhombic dodecahedron. The atomic force microscopy (AFM) study on the structure of OBs at different stages of growth in insect cells revealed polyhedrin assembly and thickness of PEP layers. The thickness of PEP layers at 53 h post-transfection (hpt) ranged from 56 to 80 nm. Mature PEP layers filled up approximately one third of the OB volume. The size of ODV nucleocapsid was found to be 433 ± 10 nm in length. The zeta potential and particle size distribution study of viruses revealed the protective role of PEP layers. The presence of a multilayered PEP confers a viable advantage to the baculoviruses compared to single-layered PEP. Thus, these findings may help in developing PEP layer-based biopolymers for protein-based nanodevices, nanoelectrodes and more stable biopesticides.
Peripheral nervous system insulin resistance in ob/ob mice
2013-01-01
Background A reduction in peripheral nervous system (PNS) insulin signaling is a proposed mechanism that may contribute to sensory neuron dysfunction and diabetic neuropathy. Neuronal insulin resistance is associated with several neurological disorders and recent evidence has indicated that dorsal root ganglion (DRG) neurons in primary culture display altered insulin signaling, yet in vivo results are lacking. Here, experiments were performed to test the hypothesis that the PNS of insulin-resistant mice displays altered insulin signal transduction in vivo. For these studies, nondiabetic control and type 2 diabetic ob/ob mice were challenged with an intrathecal injection of insulin or insulin-like growth factor 1 (IGF-1) and downstream signaling was evaluated in the DRG and sciatic nerve using Western blot analysis. Results The results indicate that insulin signaling abnormalities documented in other “insulin sensitive” tissues (i.e. muscle, fat, liver) of ob/ob mice are also present in the PNS. A robust increase in Akt activation was observed with insulin and IGF-1 stimulation in nondiabetic mice in both the sciatic nerve and DRG; however this response was blunted in both tissues from ob/ob mice. The results also suggest that upregulated JNK activation and reduced insulin receptor expression could be contributory mechanisms of PNS insulin resistance within sensory neurons. Conclusions These findings contribute to the growing body of evidence that alterations in insulin signaling occur in the PNS and may be a key factor in the pathogenesis of diabetic neuropathy. PMID:24252636
AVIRIS calibration and application in coastal oceanic environments
NASA Technical Reports Server (NTRS)
Carder, Kendall L.
1992-01-01
The Airborne Visible-Infrared Imaging Spectrometer (AVIRIS) is a test-bed for future spacecraft sensors such as the High-Resolution Imaging Spectrometer and the Moderate-Resolution Imaging Spectrometers planned for the Earth Observing System. To use this sensor for ocean applications, S/N was increased by spatial averaging of images. Post-flight recalibration was accomplished using in situ the water-leaving radiance measured at flight time, modeling radiance transmission to the aircraft, and adding modeled atmospheric radiance to that value. The preflight calibration curve was then adjusted until aircraft and modeled total radiance values matched. Water-leaving radiance values from the recalibrated AVIRIS imagery were consistent with in situ data supporting the validity of the approach. Imagery of the absorption coefficient at 415 nm and backscattering coefficient at 671 nm were used to depict the dissolved and particulate constituents of an ebb-tidal esturance plume on the East coast of Florida.
NASA Technical Reports Server (NTRS)
Lubin, Dan
2001-01-01
This study has used a combination of ocean color, backscattered ultraviolet, and passive microwave satellite data to investigate the impact of the springtime Antarctic ozone depletion on the base of the Antarctic marine food web - primary production by phytoplankton. Spectral ultraviolet (UV) radiation fields derived from the satellite data are propagated into the water column where they force physiologically-based numerical models of phytoplankton growth. This large-scale study has been divided into two components: (1) the use of Total Ozone Mapping Spectrometer (TOMS) and Special Sensor Microwave Imager (SSM/I) data in conjunction with radiative transfer theory to derive the surface spectral UV irradiance throughout the Southern Ocean; and (2) the merging of these UV irradiances with the climatology of chlorophyll derived from SeaWiFS data to specify the input data for the physiological models.
C-band polarimetric scatterometer for soil studies
NASA Astrophysics Data System (ADS)
D'Alessio, Angelo C.; Mongelli, Antonio; Notarnicola, Claudia; Paparella, Giuseppina; Posa, Francesco; Sabatelli, Vincenzo
2003-03-01
The aim of this study is to evaluate the performances of a polarimetric scatterometer. This sensor can measure the module of the electromagnetic backscattering matrix elements. The knowledge of this matrix permits the computation of all the possible polarisation combinations of transmitted and received signals through a Polarisation Synthesis approach. Scatterometer data are useful for monitoring a large number of soil physical parameters. In particular, the sensitivity of a C-band radar to different growing conditions of vegetation depends on the wave polarisation. As consequences, the possibility of acquiringi both polarisation components presents a great advantage in the vegetarian studies. In addition, this type of ground sensor can permit a fast coverage of the areas of interest. A first test of the polarimetric scatterometer has been performed over an asphalt surface, which has a well-known electromagnetic response. Moreover, a calibration procedure has been tested using both passive (Trihedral Corner Reflector, TCR) and active (Active Radar Calibrator, ARC) radar calibrator.
Optical dynamic deformation measurements at translucent materials.
Philipp, Katrin; Koukourakis, Nektarios; Kuschmierz, Robert; Leithold, Christoph; Fischer, Andreas; Czarske, Jürgen
2015-02-15
Due to their high stiffness-to-weight ratio, glass fiber-reinforced polymers are an attractive material for rotors, e.g., in the aerospace industry. A fundamental understanding of the material behavior requires non-contact, in-situ dynamic deformation measurements. The high surface speeds and particularly the translucence of the material limit the usability of conventional optical measurement techniques. We demonstrate that the laser Doppler distance sensor provides a powerful and reliable tool for monitoring radial expansion at fast rotating translucent materials. We find that backscattering in material volume does not lead to secondary signals as surface scattering results in degradation of the measurement volume inside the translucent medium. This ensures that the acquired signal contains information of the rotor surface only, as long as the sample surface is rough enough. Dynamic deformation measurements of fast-rotating fiber-reinforced polymer composite rotors with surface speeds of more than 300 m/s underline the potential of the laser Doppler sensor.
NASA Technical Reports Server (NTRS)
Brucks, J. T.; Leming, T. D.; Jones, W. L.
1980-01-01
Sea surface wind stress measurements recorded by a sonic anemometer are correlated with airborne scatterometer measurements of ocean roughness (cross section of radar backscatter) to establish the accuracy of remotely sensed data and assist in the definition of geophysical algorithms for the scatterometer sensor aboard Seasat A. Results of this investigation are as follows: Comparison of scatterometer and sonic anemometer wind stress measurements are good for the majority of cases; however, a tendency exists for scatterometer wind stress to be somewhat high for higher wind conditions experienced in this experiment (6-9 m/s). The scatterometer wind speed algorithm tends to overcompute the higher wind speeds by approximately 0.5 m/s. This is a direct result of the scatterometer overestimate of wind stress from which wind speeds are derived. Algorithmic derivations of wind speed and direction are, in most comparisons, within accuracies defined by Seasat A scatterometer sensor specifications.
Recent Seismic Experiments of OBS in the South China Sea
NASA Astrophysics Data System (ADS)
Ruan, A.; Li, J.; Wu, Z.
2012-12-01
Since 2006 some research institutions of China have carried out some important seismic experiments by using ocean bottom seismometer(OBS) in the South China Sea (SCS) and obtained many concrete progresses in modeling the crustal structure of SCS and also in understanding of its formation and evolution as well. In 2006 three wide-angle profiles were completed in the northern margin, named OBS2006-1 across the northwestern sub-basin, OBS2006-2 parallel to the sea basin boundary and OBS2006-3 across the Dongsha Rise and Chaoshan Depression respectively. In 2010 two wide- angle profiles were completed, named OBS2010-1 and OBS 2010-2 both perpendicular to the northern off-shore faulting system. During 2009-2011 four wide-angle profiles were completed in the southern margin, named OBS973-1 from southern margin to the southwestern sub-basin, OBS973-2 from Liyue Bank to the southwestern sub-basin and OBS973-3 from Xisha to the southwestern sub-basin, OBS2011-2 from Xisha to Hainan Island respectively. In 2011 two 3D seismic array of OBS were completed in the Zhongnan-Changlong sea mount chain and Huangyan-Zhenbei sea mount chain respectively. Here we present some primary but important results as follows. (1) The velocity model of OBS2006-1 indicates that the crust under the continental slope decreases from 21km to 11km, and to 7.7km in the northwestern sub-basin with Moho depth ascends from 21km to 11km. The tectonic geometry and velocity structure of the northwestern sub-basin and its margins on both sides shows symmetrical and conjugate and indicates pure shear mode of continental margin rifting mechanism. (2) The velocity model of OBS2006-3 reveals remarkable thickness with maximum 8 km of the Mesozoic sediment in Chaoshan Depression in which velocity increases downward from 4.4 km/s at top to 5.3 km/s at the bottom. The buried depth of Moho decreases from 24-25 km under Dongsha Rise to 17 km in the lower slope and an obviously velocity abnormal is detected in the upper crust of the Dongsha Rise and its velocity raises to 6.9 km/s due to the mantle underplating and magma activities. A high velocity layer (HVL) of 3-12 km thick is found in the lower crust, and its velocity is 7.1-7.4 km/s. (3) The wide angle seismic profile-OBS973-2 that extends in NW-SE direction 369km long from the northeastern Liyue Bank to the central sub-basin. It indicates that there are some small volcanoes on the top of crust in Liyue Bank and P wave velocities downward increase from 5.5~6.4km/s in the upper crust (9~10km thick) to 6.6~7.1km/s in the lower crust (11km thick). In the transition zone and sea basin P wave velocities downward increase from 5.9~6.1km/s in the upper curst (4~5km thick) to 6.6~6.9km/s in the lower crust (2~4km thick). The buried depth of Moho is 23km in Liyue Bank and 8~12km in the sea basin. The comparison of profile OBS973-2 with profile OBS2006-1 in the northern margin shows remarkable similarity between them and suggests a possibility of conjugation relationship between Liyue Bank and Zhongsha Massif. Acknowledgements This work was supported by the National Natural Science Foundation of China (91028006,40876035,41106053 and 41176046) and the National Basic Research Program of China (2007CB411701) .
NASA Technical Reports Server (NTRS)
Miller, John; Freemantle, Jim; Nickeson, Jaime (Editor); Hall, Forrest G. (Editor); Smith, David E. (Technical Monitor)
2000-01-01
The Remote Sensing Science (RSS)-19 team collected Compact Airborne Spectrographic Imager (CASI) images from the Chieftain Navaho aircraft in order to observe the seasonal change in the radiometric reflectance properties of the boreal forest landscape. CASI was deployed as a site-specific optical sensor during Boreal Ecosystem-Atmosphere Study (BOREAS) field campaigns. Image data were collected with CASI on 36 days during five field campaigns between February and September 1994, primarily at flux tower sites located at study sites near Thompson, Manitoba, and Prince Albert, Saskatchewan. A variety of CASI data collection strategies were used to meet the following scientific objectives: 1) canopy bidirectional reflectance, 2) canopy biochemistry, 3) spatial variability, and 4) estimates of up and downwelling Photosynthetically Active Radiation (PAR) and spectral albedo, as well as changes along transects across lakes and transects the Northern Study Area (NSA) and Southern Study Area (SSA). The images are stored as binary image files. A subset of the 1994 CASI acquisitions have been compressed and included on the BOREAS CD-ROM set. This subset includes three images for the NSA-OBS (Old Black Spruce) site on 06 Jun 94, 08 Aug 94, and 06 Sep 94, one image for the SSA-OBS site on 24 Jul 94; and one image for the NSA-Fen site on 08 Aug 94. The CASI imagery on the BOREAS CD-ROMs have been compressed using the Gzip program. The rest of the 1994 BOREAS CASI archive are not contained on the BOREAS CD-ROM set. Inventory listing files are supplied on the CD-ROM to inform users of the data that were collected. The RSS-19 1994 CASI images are available from the Earth Observing System Data and Information System (EOSDIS) Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC). The data files are available on a CD-ROM (see document number 20010000884).
NASA Astrophysics Data System (ADS)
Beauducel, François; Bosson, Alexis; Randriamora, Frédéric; Anténor-Habazac, Christian; Lemarchand, Arnaud; Saurel, Jean-Marie; Nercessian, Alexandre; Bouin, Marie-Paule; de Chabalier, Jean-Bernard; Clouard, Valérie
2010-05-01
Seismological and Volcanological observatories have common needs and often common practical problems for multi disciplinary data monitoring applications. In fact, access to integrated data in real-time and estimation of measurements uncertainties are keys for an efficient interpretation, but instruments variety, heterogeneity of data sampling and acquisition systems lead to difficulties that may hinder crisis management. In Guadeloupe observatory, we have developed in the last years an operational system that attempts to answer the questions in the context of a pluri-instrumental observatory. Based on a single computer server, open source scripts (Matlab, Perl, Bash, Nagios) and a Web interface, the system proposes: an extended database for networks management, stations and sensors (maps, station file with log history, technical characteristics, meta-data, photos and associated documents); a web-form interfaces for manual data input/editing and export (like geochemical analysis, some of the deformation measurements, ...); routine data processing with dedicated automatic scripts for each technique, production of validated data outputs, static graphs on preset moving time intervals, and possible e-mail alarms; computers, acquisition processes, stations and individual sensors status automatic check with simple criteria (files update and signal quality), displayed as synthetic pages for technical control. In the special case of seismology, WebObs includes a digital stripchart multichannel continuous seismogram associated with EarthWorm acquisition chain (see companion paper Part 1), event classification database, location scripts, automatic shakemaps and regional catalog with associated hypocenter maps accessed through a user request form. This system leads to a real-time Internet access for integrated monitoring and becomes a strong support for scientists and technicians exchange, and is widely open to interdisciplinary real-time modeling. It has been set up at Martinique observatory and installation is planned this year at Montserrat Volcanological Observatory. It also in production at the geomagnetic observatory of Addis Abeba in Ethiopia.
Frankfurt Medical Department Activity (FMEDDAC) OB/GYN Clinic Study
1993-08-01
ideas took hold and flourished in post-World War II Japan ( Arikan , 1990). The Japanese institutionalized the following chain FMEDDAC OB-GYN 8 reaction...be required for most organizations. FMEDDAC OB-GYN 69 References Arikan , V.L. (1991). Total quality management - Applications to nursing service
Long term seismic observation using ocean bottom seismographs in Marmara Sea, Turkey
NASA Astrophysics Data System (ADS)
Takahashi, N.; Pinar, A.; Kalafat, D.; Yamamoto, Y.; Citak, S.; Comoglu, M.; Çok, Ö.; Ogutcu, Z.; Suvarikli, M.; Tunc, S.; Gurbuz, C.; Ozel, N.; Kaneda, Y.
2015-12-01
The North Anatolian Fault crosses the Marmara Sea with a direction of E-W. There are many large earthquakes repeatedly along the fault with a linkage each other. Due to recent large eastern Aegean earthquake with M6, the Marmara Sea is the "blank zone". Japan and Turkey have a SATREPS collaborative study to clarify the structural characters, construct fault models, simulate the strong motion and tsunami, evaluate these risks with hazard maps and educate disaster prevention for local governments and residents. Our activity is one of the most basic studies, and the objectives are to clarify hypocenter locations, monitor the move, and construct fault models referring seismic/magnetotelluric structures, geodetic nature and trenching works. The target area is from western Marmara Sea to the off Istanbul area along the north Anatolian Fault. We deployed ten Ocean Bottom Seismographs (OBSs) between the Tekirdag Basin and the Central Basin in September, 2014. Then, we added five Japanese OBSs and deployed them at the western end of the Marmara Sea and the eastern Central Basin to extend observed area in March, 2015. The OBS has a three-component velocity sensor with a natural frequency of 4.5 Hz and a hydrophone. Japanese team have clarified seismicity around Japan using the OBS. The magnitude of the detected events is 1.0-1.5. We retrieved all 15 OBSs in July, 2015 and deployed them again on the same locations after data copy and battery maintenance. We started OBS data analysis combined with land stations data. Now we detect events automatically using these data and succeeded detection of over one thousand around the north Anatolian Fault. The tentative results show heterogeneous seismicity. The western and central basins have relative high seismicity and the seismogenic zone becomes thicker rather than previous estimation. Then we will evaluate hypocenter locations with high resolution and discuss the shape of faults in each segment and their linkage.
Organic brain syndrome. The psychiatric imposter.
Dubin, W R; Weiss, K J; Zeccardi, J A
1983-01-07
Rapid differentiation of organic brain syndrome (OBS) from functional psychiatric illness can be difficult when patients come to an emergency department with predominantly psychiatric symptoms. Using four screening criteria-disorientation, abnormal vital signs, clouded consciousness, and patients older than 40 years with no previous psychiatric history-we detected 38 cases of OBS from 1,140 medically cleared patients. Twelve of these patients were subsequently admitted to a medical unit. Despite the presence of striking behavioral aberration, an increased awareness of the clinical manifestations of OBS will enhance the physician's ability to discriminate OBS from other psychiatric illness.
Obstetrics Hospitalists: Risk Management Implications.
Veltman, Larry
2015-09-01
The concept of having an in-house obstetrician (serving as an obstetrics [OB] hospitalist) available 24 hours a day, 7 days a week provides a safety net for OB events that many need immediate intervention for a successful outcome. A key precept of risk management, that of loss prevention, fits perfectly with the addition of an OB hospitalist role in the perinatal department. Inherent in the role of OB hospitalists are the patient safety and risk management principles of improved communication, enhanced readiness, and immediate availability. Copyright © 2015 Elsevier Inc. All rights reserved.
Additional red and reddened stars in Cyg OB2 association
NASA Technical Reports Server (NTRS)
Parthasarathy, M.; Jain, S. K.
1989-01-01
Several new red and reddened stars are detected in the most heavily reddened associations Cyg OB2. About 47 IRAS sources are detected in Cyg OB2. Their flux distributions, and colors, suggest that they are young stellar objects embedded in dust envelopes or disks (some of them may be proto stars) and are most likely members of the Cyg OB2 association. The large values of the flux ratio L sub IR/L sub VIS suggests that the central objects are obscured because of very large extinction.
The Organic Brain Syndrome (OBS) scale: a systematic review.
Björkelund, Karin Björkman; Larsson, Sylvia; Gustafson, Lars; Andersson, Edith
2006-03-01
The Organic Brain Syndrome (OBS) Scale was developed to determine elderly patients' disturbances of awareness and orientation as to time, place and own identity, and assessment of various emotional and behavioural symptoms appearing in delirium, dementia and other organic mental diseases. The aim of the study was to examine the OBS Scale, using the eight criteria and guidelines formulated by the Scientific Advisory Committee of the Medical Outcomes Trust (SAC), and to investigate its relevance and suitability for use in various clinical settings. Systematic search and analysis of papers (30) on the OBS Scale were carried out using the criteria suggested by the SAC. The OBS Scale in many aspects satisfies the requirements suggested by the SAC: conceptual and measurement model, reliability, validity, responsiveness, interpretability, respondent and administrative burden, alternative forms of administration, and cultural and language adaptations, but there is a need for additional evaluation, especially with regard to different forms of reliability, and the translation and adaptation to other languages. The OBS Scale is a sensitive scale which is clinically useful for the description and long-term follow-up of patients showing symptoms of acute confusional state and dementia. Although the OBS Scale has been used in several clinical studies there is need for further evaluation.
Towards Experiments to Test Violation of the Original Bell Inequality
NASA Astrophysics Data System (ADS)
Khrennikov, Andrei; Basieva, Irina
2018-04-01
The aim of this note is to attract attention of experimenters to the original Bell (OB) inequality which was shadowed by the common consideration of the CHSH inequality. There are two reasons to test the OB inequality and not the CHSH inequality. First of all, the OB inequality is a straightforward consequence to the EPR-argumentation. And only this inequality is related to the EPR-Bohr debate. However, the last statement can be objected by some experts working in quantum foundations. Therefore to convince experimenters to perform the OB-violation experiment, we prefer to concentrate on the second distinguishing feature of the OB inequality which was emphasized by I. Pitowsky. He pointed out that the OB inequality provides a higher degree of violations of classicality than the CHSH inequality. The main problem is that the OB inequality is derived under the assumption of perfect (anti-) correlations. However, the last years were characterized by the amazing development of quantum technologies. Nowadays, there exist sources producing with very high probability the pairs of photons in the singlet state. Moreover, the efficiency of photon detectors was improved tremendously. In any event one can start by proceeding with the fair sampling assumption. Another possibility is to use the scheme of Hensen et al. experiment for entangled electrons.
Body fat mass, leptin and puberty.
Kiess, W; Müller, G; Galler, A; Reich, A; Deutscher, J; Klammt, J; Kratzsch, J
2000-07-01
Leptin, the ob gene product, provides a molecular basis for the lipostatic theory of the regulation of energy balance. Leptin circulates as a monomeric 16 kDa protein in rodent and human plasma and is also bound to leptin binding proteins that may form large high molecular weight complexes. Initial models of leptin action included leptin-deficient ob/ob mice and leptin-insensitive db/db mice. Peripheral or central administration of leptin reduced body weight, adiposity, and food intake in ob/ob mice but not in db/db mice. In ob/ob mice leptin treatment restored fertility. Leptin interacts with many messenger molecules in the brain. For example, leptin suppresses neuropeptide Y (NPY) expression in the arcuate nucleus. Increased NPY activity has an inhibitory effect on the gonadotropin axis and represents a direct mechanism for inhibiting sexual maturation and reproductive function in conditions of food restriction and/or energy expenditure. By modulating the hypothalamo-pituitary-gonadal axis both directly and indirectly, leptin may thus serve as the signal from fat to the brain about the adequacy of fat stores for pubertal development and reproduction. Normal leptin secretion is necessary for normal reproductive function to proceed and leptin may be a signal allowing for the point of initiation of and progression toward puberty.
Lgl1 Is Required for Olfaction and Development of Olfactory Bulb in Mice.
Li, Zhenzu; Zhang, Tingting; Lin, Zhuchun; Hou, Congzhe; Zhang, Jian; Men, Yuqin; Li, Huashun; Gao, Jiangang
2016-01-01
Lethal giant larvae 1 (Lgl1) was initially identified as a tumor suppressor in Drosophila and functioned as a key regulator of epithelial polarity and asymmetric cell division. In this study, we generated Lgl1 conditional knockout mice mediated by Pax2-Cre, which is expressed in olfactory bulb (OB). Next, we examined the effects of Lgl1 loss in the OB. First, we determined the expression patterns of Lgl1 in the neurogenic regions of the embryonic dorsal region of the LGE (dLGE) and postnatal OB. Furthermore, the Lgl1 conditional mutants exhibited abnormal morphological characteristics of the OB. Our behavioral analysis exhibited greatly impaired olfaction in Lgl1 mutant mice. To elucidate the possible mechanisms of impaired olfaction in Lgl1 mutant mice, we investigated the development of the OB. Interestingly, reduced thickness of the MCL and decreased density of mitral cells (MCs) were observed in Lgl1 mutant mice. Additionally, we observed a dramatic loss in SP8+ interneurons (e.g. calretinin and GABAergic/non-dopaminergic interneurons) in the GL of the OB. Our results demonstrate that Lgl1 is required for the development of the OB and the deletion of Lgl1 results in impaired olfaction in mice.
Lgl1 Is Required for Olfaction and Development of Olfactory Bulb in Mice
Li, Zhenzu; Zhang, Tingting; Lin, Zhuchun; Hou, Congzhe; Zhang, Jian; Men, Yuqin; Li, Huashun
2016-01-01
Lethal giant larvae 1 (Lgl1) was initially identified as a tumor suppressor in Drosophila and functioned as a key regulator of epithelial polarity and asymmetric cell division. In this study, we generated Lgl1 conditional knockout mice mediated by Pax2-Cre, which is expressed in olfactory bulb (OB). Next, we examined the effects of Lgl1 loss in the OB. First, we determined the expression patterns of Lgl1 in the neurogenic regions of the embryonic dorsal region of the LGE (dLGE) and postnatal OB. Furthermore, the Lgl1 conditional mutants exhibited abnormal morphological characteristics of the OB. Our behavioral analysis exhibited greatly impaired olfaction in Lgl1 mutant mice. To elucidate the possible mechanisms of impaired olfaction in Lgl1 mutant mice, we investigated the development of the OB. Interestingly, reduced thickness of the MCL and decreased density of mitral cells (MCs) were observed in Lgl1 mutant mice. Additionally, we observed a dramatic loss in SP8+ interneurons (e.g. calretinin and GABAergic/non-dopaminergic interneurons) in the GL of the OB. Our results demonstrate that Lgl1 is required for the development of the OB and the deletion of Lgl1 results in impaired olfaction in mice. PMID:27603780
High-accuracy fiber-optic shape sensing
NASA Astrophysics Data System (ADS)
Duncan, Roger G.; Froggatt, Mark E.; Kreger, Stephen T.; Seeley, Ryan J.; Gifford, Dawn K.; Sang, Alexander K.; Wolfe, Matthew S.
2007-04-01
We describe the results of a study of the performance characteristics of a monolithic fiber-optic shape sensor array. Distributed strain measurements in a multi-core optical fiber interrogated with the optical frequency domain reflectometry technique are used to deduce the shape of the optical fiber; referencing to a coordinate system yields position information. Two sensing techniques are discussed herein: the first employing fiber Bragg gratings and the second employing the intrinsic Rayleigh backscatter of the optical fiber. We have measured shape and position under a variety of circumstances and report the accuracy and precision of these measurements. A discussion of error sources is included.
A system analysis of the 13.3 GHz scatterometer. [antenna patterns and signal transmission
NASA Technical Reports Server (NTRS)
Wang, J. R.
1977-01-01
The performance of the 13.3 GHz airborne scatterometer system which is used as a microwave remote sensor to detect moisture content of soil is analyzed with respect to its antenna pattern, the signal flow in the receiver data channels, and the errors in the signal outputs. The operational principle and the sensitivity of the system, as well as data handling are also described. The dielectric property of the terrain surface, as far as the scatterometer is concerned, is contained in the assumed forms of the functional dependence of the backscattering coefficient of the incident angle.
Review of progress in quantitative NDE
NASA Astrophysics Data System (ADS)
s of 386 papers and plenary presentations are included. The plenary sessions are related to the national technology initiative. The other sessions covered the following NDE topics: corrosion, electromagnetic arrays, elastic wave scattering and backscattering/noise, civil structures, material properties, holography, shearography, UT wave propagation, eddy currents, coatings, signal processing, radiography, computed tomography, EM imaging, adhesive bonds, NMR, laser ultrasonics, composites, thermal techniques, magnetic measurements, nonlinear acoustics, interface modeling and characterization, UT transducers, new techniques, joined materials, probes and systems, fatigue cracks and fracture, imaging and sizing, NDE in engineering and process control, acoustics of cracks, and sensors. An author index is included.
NASA Technical Reports Server (NTRS)
Moore, R. K.; Ulaby, F. T. (Principal Investigator); Barr, J. C.; Sobti, A.
1974-01-01
The author has identified the following significant results. Terrain microwave backscatter and emission response to soil moisture variations were investigated using Skylab's 13.9 GHz RADSCAT (radiometer/scatterometer) system. Data acquired on June 5, 1973, over a test site in west-central Texas indicated a fair degree of correlation with composite rainfall. The scan made was cross-track contiguous (CTC) with a pitch of 29.4 deg and no roll effect. Vertical polarization was employed with both radiometer and scatterometer. The composite rainfall was computed according to the flood prediction technique using rainfall data supplied by weather reporting stations.
Wang, Yue-jing; Huang, Su-ling; Feng, Ying; Ning, Meng-meng; Leng, Ying
2012-01-01
Aim: Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a potent and selective inhibitor of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) with the ability to ameliorate metabolic disorders in diet-induced obese mice. In the present study, we investigated the effects of emodin on adipocyte function and the underlying mechanisms in vitro, and its anti-diabetic effects in ob/ob mice. Methods: 3T3-L1 adipocytes were used for in vitro studies. 11β-HSD1A activity was evaluated with a scintillation proximity assay. The adipogenesis, glucose uptake, lipolysis and adiponectin secretion were investigated in 3T3-L1 adipocytes treated with emodin in the presence of active (corticosterone) or inactive glucocorticoid (11-dehydrocorticosterone). For in vivo studies, ob/ob mice were administered emodin (25 and 50 mg·kg−1·d−1, ip) for 26 d. On the last day of administration, the serum was collected and the mesenteric and perirenal fat were dissected for analyses. Results: Emodin inhibited the 11β-HSD1 activity in 3T3-L1 adipocytes in concentration- and time-dependent manners (the IC50 values were 7.237 and 4.204 μmol/L, respectively, after 1 and 24 h treatment. In 3T3-L1 adipocytes, emodin (30 μmol/L) suppressed 11-dehydrocorticosterone-induced adipogenesis without affecting corticosterone-induced adipogenesis; emodin (3 μmol/L) reduced 11-dehydrocorticosterone-stimulated lipolysis, but had no effect on corticosterone-induced lipolysis. Moreover, emodin (3 μmol/L) partly reversed the impaired insulin-stimulated glucose uptake and adiponectin secretion induced by 11-dehydrocorticosterone but not those induced by corticosterone. In ob/ob mice, long-term emodin administration decreased 11β-HSD1 activity in mesenteric adipose tissues, lowered non-fasting and fasting blood glucose levels, and improved glucose tolerance. Conclusion: Emodin improves the inactive glucocorticoid-induced adipose tissue dysfunction by selective inhibition on 11β-HSD1 in adipocyte in vitro and improves glycemic control in ob/ob mice. PMID:22922341
Musser, A M; Archer, M
1998-01-01
A reconstruction of the skull, dentary and dentition of the middle Miocene ornithorhynchid Obdurodon dicksoni has been made possible by acquisition of nearly complete cranial and dental material. Access to new anatomical work on the living platypus, Ornithorhynchus anatinus, and the present comparative study of the cranial foramina of Ob. dicksoni and Or. anatinus have provided new insights into the evolution of the ornithorhynchid skull. The hypertrophied bill in Ob. dicksoni is seen here as possibly apomorphic, although evidence from ontogenetic studies of Or. anatinus suggests that the basic form of the bill in Ob. dicksoni (where the rostral crura meet at the midline) may be ancestral to the form of the bill in Or. anatinus (where the rostral crura meet at the midline in the embryonic platypus but diverge in the adult). Differences in the relative positions of cranial structures, and in the relationships of certain cranial foramina, indicate that the cranium may have become secondarily shortened in Or. anatinus, possibly evolving from a more elongate skull type such as that of Ob. dicksoni. The plesiomorphic dentary of Ob. dicksoni, with well-developed coronoid and angular processes, contrasts with the dentary of Or. anatinus, in which the processes are almost vestigial, as well as with the dentary of the late Oligocene, congeneric Ob. insignis, in which the angular process appears to be reduced (the coronoid process is missing). In this regard the dentary of Ob. insignis seems to be morphologically closer to Or. anatinus than is the dentary of the younger Ob. dicksoni. Phylogenetic conclusions differ from previous analyses in viewing the northern Australian Ob. dicksoni as possibly derived in possessing a hypertrophied bill and dorsoventrally flattened skull and dentary, perhaps being a specialized branch of the Obdurodon line rather than ancestral to species of Ornithorhynchus. The presence of functional teeth and the robust, flattened skull and dentary in Ob. dicksoni argue for differences in diet and lifestyle between this extinct ornithorhynchid and the living Ornithorhynchus. PMID:9720105
SOBECKI, Janelle N.; CURLIN, Farr A.; RASINSKI, Kenneth A.; LINDAU, Stacy Tessler
2012-01-01
Introduction Sexuality is a key aspect of women’s physical and psychological health. Research shows both patients and physicians face barriers to communication about sexuality. Given their expertise and training in addressing conditions of the female genital tract across the female life course, obstetrician/gynecologists (ob/gyns) are well-positioned among all physicians to address sexuality issues with female patients. New practice guidelines for management of female sexual dysfunction and the importance of female sexual behavior and function to virtually all aspects of ob/gyn care, and to women’s health more broadly, warrant up-to-date information regarding ob/gyns’ sexual history-taking routine. Aims To determine obstetrician/gynecologists’ practices of communication with patients about sexuality, and to examine the individual and practice-level correlates of such communication. Methods A population-based sample of 1154 practicing U.S. obstetrician/gynecologists (53% male; mean age 48 years) was surveyed regarding their practices of communication with patients about sex. Main Outcome Measures Self-reported frequency measures of ob/gyns’ communication practices with patients including whether or not ob/gyns discuss patients’ sexual activities, sexual orientation, satisfaction with sexual life, pleasure with sexual activity, and sexual problems or dysfunction, as well as whether or not one ever expresses disapproval of or disagreement with patients’ sexual practices. Multivariable analysis was used to correlate physicians’ personal and practice characteristics with these communication practices. Results Survey response rate was 65.6%. Sixty-three percent of ob/gyns reported routinely assessing patients’ sexual activities; 40% routinely asked about sexual problems. Fewer asked about sexual satisfaction (28.5%), sexual orientation/identity (27.7%), or pleasure with sexual activity (13.8%). A quarter of ob/gyns reported they had expressed disapproval of patients’ sexual practices. Ob/gyns practicing predominately gynecology were significantly more likely than other ob/gyns to routinely ask about each of the five outcomes investigated. Conclusion The majority of U.S. ob/gyns report routinely asking patients about their sexual activities, but most other areas of patients’ sexuality are not routinely discussed. PMID:22443146
Choudhary, Shilpa; Blackwell, Katherine; Voznesensky, Olga; Roy, Abhijit Deb; Pilbeam, Carol
2014-01-01
Intermittent PTH is the major anabolic therapy for osteoporosis while continuous PTH causes bone loss. PTH acts on the osteoblast (OB) lineage to regulate bone resorption and formation. PTH also induces cyclooxygenase-2 (COX-2), producing prostaglandin E2 (PGE2) that can act on both OBs and osteoclasts (OCs). Because intermittent PTH is more anabolic in Cox-2 knockout (KO) than wild type (WT) mice, we hypothesized COX-2 might contribute to the effects of continuous PTH by suppressing PTH-stimulated differentiation of mesenchymal stem cells into OBs. We compared effects of continuous PTH on bone marrow stromal cells (BMSCs) and primary OBs (POBs) from Cox-2 KO mice, mice with deletion of PGE2 receptors (Ptger4 and Ptger2 KO mice), and WT controls. PTH increased OB differentiation in BMSCs only in the absence of COX-2 expression or activity. In the absence of COX-2, PTH stimulated differentiation if added during the first week of culture. In Cox-2 KO BMSCs, PTH-stimulated differentiation was prevented by adding PGE2 to cultures. Co-culture of POBs with M-CSF-expanded bone marrow macrophages (BMMs) showed that the inhibition of PTH-stimulated OB differentiation required not only COX-2 or PGE2 but also BMMs. Sufficient PGE2 to mediate the inhibitory effect was made by either WT POBs or WT BMMs. The inhibitory effect mediated by COX-2/PGE2 was transferred by conditioned media from RANKL-treated BMMs and could be blocked by osteoprotegerin, which interferes with RANKL binding to its receptor on OC lineage cells. Deletion of Ptger4, but not Ptger2, in BMMs prevented the inhibition of PTH-stimulated OB differentiation. As expected, PGE2 also stimulated OB differentiation, but when given in combination with PTH, the stimulatory effects of both were abrogated. These data suggest that PGE2, acting via EP4R on BMMs committed to the OC lineage, stimulated secretion of a factor or factors that acted to suppress PTH-stimulated OB differentiation. This suppression of OB differentiation could contribute to the bone loss seen with continuous PTH in vivo. PMID:23639875
Broad-band BOS (BBYB) development and calibration in Taiwan
NASA Astrophysics Data System (ADS)
Lin, C. R.; Wang, C. C.; Kuo, B. Y.; Chen, P.; Jang, J. P.; Chang, H.; Laio, Y. C.; Chang, K. H.; Lin, F. S.
2016-12-01
Since 2009, combine with Academia Sinica, National Applied Research Laboratories and National Sun Yat-sen University formed ocean bottom seismograph (OBS) development team to develop sub-broadband OBS (called Yardbird OBS). Through a series deploy experiment at seafloor offshore Taiwan that got a lot of data can be used to study plate tectonics, seismic activity, source characteristics. Nowadays they have pretty good results already. Due to bandwidth limitations of the Yardbird OBS that inadequate to use for analyze global-scale earthquake. Therefor developing broadband ocean bottom seismograph is an important goal for the development team. Currently the broadband OBS (called BBYB) design and construction have completed the initial experiment phase. Due to underwater instruments always got high risk. Something accidentally making equipment sank in the sea cannot be recovery. Even recovery of equipment may also be causing poor performance because there is no data record. It cannot be to accomplish the experiment mission. In order to improve the OBS performance, avoid OBS dis-recovery or data collection is incomplete, must be sure all OBS's each component (such as seismic data recording device, balanced body, sonar dashboard, instruments and internal wiring ...) with well-done quality before assembly. Each component could go through very rigorous testing, strict and pick out the good components in the assembly process. Be sure all of produce the OBS under the water after a long deployment could successful recovery and got valuable data. In this presentation we will show a serial testing procedure and results for quality each BBYB component. Such as: Data logger: digitizer sensitivity, sampling rate, clock timing. Acoustic controller: function of Enable, Disable, Range, Release 1, Release 2, Option 1(Release disable). Air pressure gauge for glass ball: accuracy. A check lists of connector wiring check for assembling instrument. Design deploy and recovery procedure for operation on deck. We hope through repeated testing to ensure perfect performance of BBYB and apply the testing concept to another equipment for improve instruments performance. Keywords: ocean bottom seismograph (OBS); Data logger; Acoustic controller; Air pressure gauge.
NASA Astrophysics Data System (ADS)
McIntyre, M. L.; Naar, D. F.; Carder, K. L.; Howd, P. A.; Lewis, J. M.; Donahue, B. T.; Chen, F. R.
2002-12-01
There is growing interest in applying optical remote sensing techniques to shallow-water geological applications such as bathymetry and bottom characterization. Model inversions of hyperspectral remote-sensing reflectance imagery can provide estimates of bottom albedo and depth. This research was conducted in support of the HyCODE (Hyperspectral Coupled Ocean Dynamics Experiment) project in order to test optical sensor performance and the use of a hyperspectral remote-sensing reflectance algorithm for shallow waters in estimating bottom depths and reflectance. The objective of this project was to compare optically derived products of bottom depths and reflectance to shipborne acoustic measurements of bathymetry and backscatter. A set of three high-resolution, multibeam surveys within an 18 km by 1.5 km shore-perpendicular transect 5 km offshore of Sarasota, Florida were collected at water depths ranging from 8 m to 16 m. These products are compared to bottom depths derived from aircraft remote-sensing data collected with the AVIRIS (Airborne Visible-Infrared Imaging Spectrometer) instrument data by means of a semi-analytical remote sensing reflectance model. The pixel size of the multibeam bathymetry and AVIRIS data are 0.25 m and 10 m, respectively. When viewed at full resolution, the multibeam bathymetry data show small-scale sedimentary bedforms (wavelength ~10m, amplitude ~1m) that are not observed in the lower resolution hyperspectral bathymetry. However, model-derived bottom depths agree well with a smoothed version of the multibeam bathymetry. Depths derived from shipborne hyperspectral measurements were accurate within 13%. In areas where diver observations confirmed biological growth and bioturbation, derived bottom depths were less accurate. Acoustic backscatter corresponds well with the aircraft hyperspectral imagery and in situ measurements of bottom reflectance. Acoustic backscatter was used to define the distribution of different bottom types. Acoustic backscatter imagery corresponds well with the AVIRIS data in the middle to outer study area, implying a close correspondence between seafloor character and optical reflectance. AVIRIS data in the inner study area show poorer correspondence with the acoustic facies, indicating greater water column effects (turbidity). Acoustic backscatter as a proxy for bottom albedo, in conjunction with multibeam bathymetry data, will allow for more precise modeling of the optical signal in coastal environments.
Macchi, Edoardo Gino; Tosi, Daniele; Braschi, Giovanni; Gallati, Mario; Cigada, Alfredo; Busca, Giorgio; Lewis, Elfed
2014-01-01
Radiofrequency thermal ablation (RFTA) induces a high-temperature field in a biological tissue having steep spatial (up to 6°C∕mm) and temporal (up to 1°C∕s) gradients. Applied in cancer care, RFTA produces a localized heating, cytotoxic for tumor cells, and is able to treat tumors with sizes up to 3 to 5 cm in diameter. The online measurement of temperature distribution at the RFTA point of care has been previously carried out with miniature thermocouples and optical fiber sensors, which exhibit problems of size, alteration of RFTA pattern, hysteresis, and sensor density worse than 1 sensor∕cm. In this work, we apply a distributed temperature sensor (DTS) with a submillimeter spatial resolution for the monitoring of RFTA in porcine liver tissue. The DTS demodulates the chaotic Rayleigh backscattering pattern with an interferometric setup to obtain the real-time temperature distribution. A measurement chamber has been set up with the fiber crossing the tissue along different diameters. Several experiments have been carried out measuring the space-time evolution of temperature during RFTA. The present work showcases the temperature monitoring in RFTA with an unprecedented spatial resolution and is exportable to in vivo measurement; the acquired data can be particularly useful for the validation of RFTA computational models.
Weighing the stigma of weight: An fMRI study of neural reactivity to the pain of obese individuals.
Azevedo, R T; Macaluso, E; Viola, V; Sani, G; Aglioti, S M
2014-05-01
Explicit negative attitudes and blameful beliefs (e.g. poor diet, laziness) towards obese individuals are well documented and are pervasive even among health professionals. Here we sought to determine whether obesity stigma is reflected in a fundamental feature of intersubjectivity namely the automatic neural resonance with others' affective experiences. During fMRI, normal-weight female participants observed short clips depicting normal-weight (NW) and obese (Ob) models experiencing pain. Importantly, participants believed that half of the Ob were overweight due to a hormonal disorder (HormOb) and ignored the cause of obesity of the remaining models (Unknown obese models; UnkOb). Analyses of hemodynamic responses showed reduced activity to the pain of Ob compared to that of NW in areas associated with pain processing and early visual processing. The comparison between the two Ob conditions revealed a further decrease of activity to HormOb's pain compared to UnkOb's (and NW) pain in the right inferior frontal gyrus, an area associated with emotional resonance. Our study demonstrates that stigma for obese individuals can be observed at implicit levels, and that it is modulated by knowledge concerning the etiology of obesity, with the seemingly surprising result that obesity due to disease may result in greater stigmatization. Moreover, the perceived similarity with the models and the ambivalent emotion of pity may index biased brain responses to obese individuals' pain. The study highlights a possibly important neural link between resonance with the pain of others and obesity stigma. Copyright © 2013 Elsevier Inc. All rights reserved.
Gum, Rebecca J; Gaede, Lori L; Heindel, Matthew A; Waring, Jeffrey F; Trevillyan, James M; Zinker, Bradley A; Stark, Margery E; Wilcox, Denise; Jirousek, Michael R; Rondinone, Cristina M; Ulrich, Roger G
2003-06-01
Phosphorylation of stress-activated kinase p38, a MAPK family member, was increased in liver of ob/ob diabetic mice relative to lean littermates. Treatment of ob/ob mice with protein tyrosine phosphatase 1B (PTP1B) antisense oligonucleotides (ASO) reduced phosphorylation of p38 in liver-to below lean littermate levels-and normalized plasma glucose while reducing plasma insulin. Phosphorylation of ERK, but not JNK, was also decreased in ASO-treated mice. PTP1B ASO decreased TNFalpha protein levels and phosphorylation of the transcription factor cAMP response element binding protein (CREB) in liver, both of which can occur through decreased phosphorylation of p38 and both of which have been implicated in insulin resistance or hyperglycemia. Decreased p38 phosphorylation was not directly due to decreased phosphorylation of the kinases that normally phosphorylate p38-MKK3 and MKK6. Additionally, p38 phosphorylation was not enhanced in liver upon insulin stimulation of ASO-treated ob/ob mice (despite increased activation of other signaling molecules) corroborating that p38 is not directly affected via the insulin receptor. Instead, decreased phosphorylation of p38 may be due to increased expression of MAPK phosphatases, particularly the p38/ERK phosphatase PAC1 (phosphatase of activated cells). This study demonstrates that reduction of PTP1B protein using ASO reduces activation of p38 and its substrates TNFalpha and CREB in liver of diabetic mice, which correlates with decreased hyperglycemia and hyperinsulinemia.
Ford, Stephen P; Zhang, Liren; Zhu, Meijun; Miller, Myrna M; Smith, Derek T; Hess, Bret W; Moss, Gary E; Nathanielsz, Peter W; Nijland, Mark J
2009-09-01
Maternal obesity affects offspring weight, body composition, and organ function, increasing diabetes and metabolic syndrome risk. We determined effects of maternal obesity and a high-energy diet on fetal pancreatic development. Sixty days prior to breeding, ewes were assigned to control [100% of National Research Council (NRC) recommendations] or obesogenic (OB; 150% NRC) diets. At 75 days gestation, OB ewes exhibited elevated insulin-to-glucose ratios at rest and during a glucose tolerance test, demonstrating insulin resistance compared with control ewes. In fetal studies, ewes ate their respective diets from 60 days before to 75 days after conception when animals were euthanized under general anesthesia. OB and control ewes increased in body weight by approximately 43% and approximately 6%, respectively, from diet initiation until necropsy. Although all organs were heavier in fetuses from OB ewes, only pancreatic weight increased as a percentage of fetal weight. Blood glucose, insulin, and cortisol were elevated in OB ewes and fetuses on day 75. Insulin-positive cells per unit pancreatic area were 50% greater in fetuses from OB ewes as a result of increased beta-cell mitoses rather than decreased programmed cell death. Lambs of OB ewes were born earlier but weighed the same as control lambs; however, their crown-to-rump length was reduced, and their fat mass was increased. We conclude that increased systemic insulin in fetuses from OB ewes results from increased glucose exposure and/or cortisol-induced accelerated fetal beta-cell maturation and may contribute to premature beta-cell function loss and predisposition to obesity and metabolic disease in offspring.
Pliego, Jose F; Wehbe-Janek, Hania; Rajab, M Hasan; Browning, Jeff L; Fothergill, Russell E
2008-01-01
To evaluate the effectiveness of an obstetrical and gynecologic (Ob/Gyn) Boot Camp simulation training on perceived technical competency, confidence in a leadership role, and stress hardiness of resident training. We conducted a prospective pilot study on the effectiveness of an Ob/Gyn Boot Camp on resident training. Residents participated in an intensive immersion in clinical simulation of common obstetrical emergencies including shoulder dystocia, neonatal resuscitation, postpartum hemorrhage, and ruptured ectopic pregnancy. After the training, residents completed a Web-based survey on their perceptions of how the Ob/Gyn Boot Camp affected their 1) technical competency in the assessment and management of their patients, 2) confidence in taking a leadership role, and 3) stress hardiness. Residents rated their perceptions on a Likert scale of 1 to 5, 1 = poor to 5 = excellent. Twenty-three (14 Ob/Gyn and 9 family medicine) residents participated in this pilot study. Eighteen (78%) residents completed the online survey; 4 Ob/Gyn and 1 family medicine resident did not complete the survey. The residents reported that the simulation training stimulated an interest in learning key skills for obstetrical and gynecologic emergencies. Ob/Gyn residents reported significant improvement in their perceived technical competence and stress hardiness after the Boot Camp. However both Ob/Gyn and family medicine residents reported no significant improvement of confidence in their leadership abilities during obstetrical emergencies after the Boot Camp. Boot Camp simulation training early in the curriculum has the potential for enhancing residents' self-assessments of confidence, competency, and stress hardiness in managing obstetrical emergencies.
Zheng, Qiao; Dunlap, Sarah M; Zhu, Jinling; Downs-Kelly, Erinn; Rich, Jeremy; Hursting, Stephen D; Berger, Nathan A; Reizes, Ofer
2011-08-01
Obesity increases both the risk and mortality associated with many types of cancer including that of the breast. In mice, obesity increases both incidence of spontaneous tumors and burden of transplanted tumors. Our findings identify leptin, an adipose secreted cytokine, in promoting increased mammary tumor burden in obese mice and provide a link between this adipokine and cancer. Using a transplantable tumor that develops spontaneously in the murine mammary tumor virus-Wnt-1 transgenic mice, we show that tumors transplanted into obese leptin receptor (LepRb)-deficient (db/db) mice grow to eight times the volume of tumors transplanted into lean wild-type (WT) mice. However, tumor outgrowth and overall tumor burden is reduced in obese, leptin-deficient (ob/ob) mice. The residual tumors in ob/ob mice contain fewer undifferentiated tumor cells (keratin 6 immunopositive) compared with WT or db/db mice. Furthermore, tumors in ob/ob mice contain fewer cells expressing phosphorylated Akt, a growth promoting kinase activated by the LepRb, compared with WT and db/db mice. In vivo limiting dilution analysis of residual tumors from ob/ob mice indicated reduced tumor initiating activity suggesting fewer cancer stem cells (CSCs). The tumor cell populations reduced by leptin deficiency were identified by fluorescence-activated cell sorting and found to express LepRb. Finally, LepRb expressing tumor cells exhibit stem cell characteristics based on the ability to form tumorspheres in vitro and leptin promotes their survival. These studies provide critical new insight on the role of leptin in tumor growth and implicate LepRb as a CSC target.
Wind collisions in three massive stars of Cygnus OB2
NASA Astrophysics Data System (ADS)
Cazorla, Constantin; Nazé, Yaël; Rauw, Gregor
2014-01-01
Aims: We wish to study the origin of the X-ray emission of three massive stars in the Cyg OB2 association: Cyg OB2 #5, Cyg OB2 #8A, and Cyg OB2 #12. Methods: To this aim, dedicated X-ray observations from XMM-Newton and Swift are used, as well as archival ROSAT and Suzaku data. Results: Our results on Cyg OB2 #8A improve the phase coverage of the orbit and confirm previous studies: the signature of a wind-wind collision is conspicuous. In addition, signatures of a wind-wind collision are also detected in Cyg OB2 #5, but the X-ray emission appears to be associated with the collision between the inner binary and the tertiary component orbiting it with a 6.7 yr period, without a putative collision inside the binary. The X-ray properties strongly constrain the orbital parameters, notably allowing us to discard some proposed orbital solutions. To improve the knowledge of the orbit, we revisit the light curves and radial velocity of the inner binary, looking for reflex motion induced by the third star. Finally, the X-ray emission of Cyg OB2 #12 is also analyzed. It shows a marked decrease in recent years, compatible with either a wind-wind collision in a wide binary or the aftermath of a recent eruption. Based on observations collected at the Observatoire de Haute Provence (OHP) as well as with Swift and XMM-Newton.Tables 1-3 and 5 are available in electronic form at http://www.aanda.org
Harsh Parenting and Food Insecurity in Adolescence: The Association With Emerging Adult Obesity.
Lohman, Brenda J; Gillette, Meghan T; Neppl, Tricia K
2016-07-01
As the first study of its kind, the overall purpose of this article was to examine the relationships and interactions between harsh parenting (HP) and food insecurity (FI) in adolescence on the development of overweight/obesity (OW/OB) in emerging adulthood. Data came from the Iowa Youth and Families Project, a longitudinal study of 451 adolescent youth and their families that began in 1989 in the rural Midwest. Adolescents were aged 13 years at the initial assessment, and weight status in emerging adulthood was measured 10 years later at age 23. Experiencing HP in adolescence predicted greater odds of OW/OB at 23 years old. Higher body mass indexes of the adolescent and his or her father in adolescence also increased the odds of being OW/OB at 23 years. Having parents with higher levels of education lowered the odds of being OW/OB in emerging adulthood. Finally, females who experienced high levels of FI and HP in adolescence had higher odds of OW/OB at 23 years in comparison to males. HP, in combination with FI in adolescence, predicted OW/OB for females in emerging adulthood but not for males. This study contributes to an understanding of the interplay between multiple influences in adolescence: namely, parenting and economic influences. Assessing antecedents to OW/OB in emerging adulthood via multiple pathways provides a more complex understanding of how and why adolescents turn into OW/OB adults. Copyright © 2016 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.
Ford, Stephen P.; Zhang, Liren; Zhu, Meijun; Miller, Myrna M.; Smith, Derek T.; Hess, Bret W.; Moss, Gary E.; Nathanielsz, Peter W.; Nijland, Mark J.
2009-01-01
Maternal obesity affects offspring weight, body composition, and organ function, increasing diabetes and metabolic syndrome risk. We determined effects of maternal obesity and a high-energy diet on fetal pancreatic development. Sixty days prior to breeding, ewes were assigned to control [100% of National Research Council (NRC) recommendations] or obesogenic (OB; 150% NRC) diets. At 75 days gestation, OB ewes exhibited elevated insulin-to-glucose ratios at rest and during a glucose tolerance test, demonstrating insulin resistance compared with control ewes. In fetal studies, ewes ate their respective diets from 60 days before to 75 days after conception when animals were euthanized under general anesthesia. OB and control ewes increased in body weight by ∼43% and ∼6%, respectively, from diet initiation until necropsy. Although all organs were heavier in fetuses from OB ewes, only pancreatic weight increased as a percentage of fetal weight. Blood glucose, insulin, and cortisol were elevated in OB ewes and fetuses on day 75. Insulin-positive cells per unit pancreatic area were 50% greater in fetuses from OB ewes as a result of increased β-cell mitoses rather than decreased programmed cell death. Lambs of OB ewes were born earlier but weighed the same as control lambs; however, their crown-to-rump length was reduced, and their fat mass was increased. We conclude that increased systemic insulin in fetuses from OB ewes results from increased glucose exposure and/or cortisol-induced accelerated fetal β-cell maturation and may contribute to premature β-cell function loss and predisposition to obesity and metabolic disease in offspring. PMID:19605766
Perceived relevance of oral biology by dental students.
Scheven, B A A
2012-02-01
This study investigated the perception that dental students have regarding the relevance of oral biology (OB) to dental education and dentistry in general. Moreover, this study analysed students' attitude towards OB learning approaches and resources. A questionnaire based on a Likert scale was used to survey pre-clinical/second (BDS2)- and final/fifth (BDS5)-year dental students at the School of Dentistry of the University of Birmingham (United Kingdom). In comparison, a small group of postgraduate specialist registrars were surveyed to evaluate the attitudes of practising dentists. The results show that all study groups expressed a high level of perceived relevance of OB to dentistry. Students' perception of OB for dental education, clinical training and practice also scored high. More than 40% of undergraduate students and about 55% of the postgraduates indicated a perceived change in their attitude towards OB with time characterised by increased appreciation of the subject. Lectures were considered as the most important teaching approach, whereas 'group poster projects' ranked lowest. Of the different study resources, lecture handouts received the overall highest importance score. The results indicate that dental students considered OB relevant for dental education and dentistry and suggest a positive attitude towards the subject. This study also suggested that dental students prefer teacher-centred/led teaching rather than student-directed learning of OB. The article addresses the role of OB and science-related research projects within the dental curriculum and discusses that close integration of basic sciences with dental education may enrich dental education and overall learning experience. © 2011 John Wiley & Sons A/S.
Lining bunker walls with oxygen barrier film reduces nutrient losses in corn silages.
Lima, L M; Dos Santos, J P; Casagrande, D R; Ávila, C L S; Lara, M S; Bernardes, T F
2017-06-01
The objective of this study was to evaluate 2 systems for covering corn silage in bunker silos. The first system consisted of a sheet of 45-μm-thick oxygen barrier film (OB, polyethylene + ethylene-vinyl alcohol) placed along the length of the sidewall before filling. After filling, the excess film was pulled over the wall on top of the silage, and a sheet of polyethylene was placed on top. The second system involved using a standard sheet (ST) of 180-μm-thick polyethylene film. Eight commercial bunker silos were divided into 2 parts lengthwise so that one-half of the silo was covered with OB and the other half with a ST system. During the filling, 3 net bags with chopped corn were buried in the central part (halfway between the top and bottom of the silo) of the bunkers (CCOR) in 3 sections 10 m apart. After filling, 18 net bags (9 per covering system) were buried 40 cm below the top surface of the 3 sections. These bags were placed at 3 distances from the bunker walls (0 to 50 cm, 51 to 100 cm, and 101 to 150 cm). During unloading, the bags were removed from the silos to determine the dry matter (DM) losses, fermentation end products, and nutritive value. The Milk2006 spreadsheet was used to estimate milk per tonne of DM. The model included the fixed effect of treatment (7 different locations in the bunker) and the random effect of the silo. Two contrasts were tested to compare silages in the top laterals (shoulders) with that in the CCOR (CCOR vs. OB and CCOR vs. ST). Three contrasts compared the corresponding distances of the silage covered by the 2 systems (OB50 vs. ST50, OB100 vs. ST100 and OB150 vs. ST150). Variables were analyzed with the PROC MIXED procedure of the SAS at the 5% level. The OB method produced well-fermented silages, which were similar to CCOR, whereas the OB system showed less lactic acid and greater pH and mold counts compared with CCOR. The ST method had 116.2 kg of milk/t less than the CCOR, as the OB system and the CCOR were similar (1,258.3 and 1,294.0 kg/t, respectively). Regarding the distances from the walls, the effects were more pronounced from 0 to 101 cm. The OB50 and OB100 silages had better quality and lower mold counts and DM losses than ST50 and ST100. The OB system reduced DM and nutrient losses at the shoulders in farm bunker corn silages compared with no sidewall plastic. The OB film should lap onto the crop for at least 200 cm so that 150 cm are covered outward from the wall. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
A Behavioral Approach to Improving Self-Care Skills in OBS Patients.
ERIC Educational Resources Information Center
McEvoy, Cathy L.; Patterson, Roger L.
Traditionally, the treatment of geriatric patients suffering from Organic Brain Syndrome (OBS) has been characterized by non-therapeutic custodial care. To determine whether elderly clients with dementia can benefit from self-care skill training, and to compare their progress with clients without OBS, 30 clients of the Residential Aging Program in…
Gelfand, Michele J; Aycan, Zeynep; Erez, Miriam; Leung, Kwok
2017-03-01
In celebration of the anniversary of the Journal of Applied Psychology ( JAP ), we take a hundred-year journey to examine how the science of cross-cultural industrial/organizational psychology and organizational behavior (CCIO/OB) has evolved, both in JAP and in the larger field. We review broad trends and provide illustrative examples in the theoretical, methodological, and analytic advances in CCIO/OB during 4 main periods: the early years (1917-1949), the middle 20th century (1950-1979), the later 20th century (1980-2000), and the 21st century (2000 to the present). Within each period, we discuss key historical and societal events that influenced the development of the science of CCIO/OB, major trends in research on CCIO/OB in the field in general and JAP in particular, and important milestones and breakthroughs achieved. We highlight pitfalls in research on CCIO/OB and opportunities for growth. We conclude with recommendations for the next 100 years of CC IO/OB research in JAP and beyond. (PsycINFO Database Record (c) 2017 APA, all rights reserved).
Briceño, C; Vivas, A K; Calvet, N; Hartmann, L; Pacheco, R; Herrera, D; Romero, L; Berlind, P; Sánchez, G; Snyder, J A; Andrews, P
2001-01-05
We are conducting a large-scale, multiepoch, optical photometric survey [Centro de Investigaciones de Astronomia-Quasar Equatorial Survey Team (CIDA-QUEST)] covering about 120 square degrees to identify the young low-mass stars in the Orion OB1 association. We present results for an area of 34 square degrees. Using photometric variability as our main selection criterion, as well as follow-up spectroscopy, we confirmed 168 previously unidentified pre-main sequence stars that are about 0.6 to 0.9 times the mass of the sun (Mo), with ages of about 1 million to 3 million years (Ori OB1b) and about 3 million to 10 million years (Ori OB1a). The low-mass stars are spatially coincident with the high-mass (at least 3 Mo) members of the associations. Indicators of disk accretion such as Halpha emission and near-infrared emission from dusty disks fall sharply from Ori OB1b to Ori OB1a, indicating that the time scale for disk dissipation and possibly the onset of planet formation is a few million years.
Jimenez-Zepeda, Victor H; Reece, Donna E; Trudel, Suzanne; Franke, Norman; Winter, Andrew; Chen, Christine; Tiedemann, Rodger; Kukreti, Vishal
2014-10-01
Abstract Recently, the occurrence of oligoclonal and monoclonal bands (OB/MB) unrelated to the original clone has been reported in patients with multiple myeloma who undergo autologous stem cell transplant (ASCT) and/or receive treatment with novel agents. The aim of our study was to assess the impact of OB/MB occurrence on overall (OS) and progression-free survival (PFS) for patients with MM undergoing single ASCT at our institution. All consecutive patients with documented MM undergoing single ASCT from January 2000 to December 2012 were evaluated. Ninety-nine patients (11.8%) developed OB/MB at day 100 post-ASCT (32.3%, OB and 67.7%, MB). Multivariate analysis identified the development of OBs/MBs as an independent favorable prognostic factor for OS and PFS (p = 0.008 and 0.012, respectively). In conclusion, the occurrence of OB/MB is an important prognostic factor in patients with MM who undergo ASCT. Its impact on clinical outcomes should be prospectively validated and its biological significance further elucidated.
Genistein treatment increases bone mass in obese, hyperglycemic mice.
Michelin, Richard M; Al-Nakkash, Layla; Broderick, Tom L; Plochocki, Jeffrey H
2016-01-01
Obesity and type 2 diabetes mellitus are associated with elevated risk of limb bone fracture. Incidences of these conditions are on the rise worldwide. Genistein, a phytoestrogen, has been shown by several studies to demonstrate bone-protective properties and may improve bone health in obese type 2 diabetics. In this study, we test the effects of genistein treatment on limb bone and growth plate cartilage histomorphometry in obese, hyperglycemic ob/ob mice. Six-week-old ob/ob mice were divided into control and genistein-treated groups. Genistein-treated mice were fed a diet containing 600 mg genistein/kg for a period of 4 weeks. Cross-sectional geometric and histomorphometric analyses were conducted on tibias. Genistein-treated mice remained obese and hyperglycemic. However, histomorphometric comparisons show that genistein-treated mice have greater tibial midshaft diameters and ratios of cortical bone to total tissue area than the controls. Genistein-treated mice also exhibit decreased growth plate thickness of the proximal tibia. Our results indicate that genistein treatment affects bone of the tibial midshaft in the ob/ob mouse, independent of improvements in the hyperglycemic state and body weight.
The daidzein- and estradiol- induced anorectic action in CCK or leptin receptor deficiency rats.
Fujitani, Mina; Mizushige, Takafumi; Bhattarai, Keshab; Iwahara, Asami; Aida, Ryojiro; Kishida, Taro
2015-01-01
We investigated the effect of daidzein feeding and estradiol treatment on food intake in cholecystokinin-1 receptor (CCK1R) deficiency, leptin receptor (ObRb) deficiency rats and their wild-type rats. These rats underwent an ovariectomy or a sham operation. For the 5 week experiment, each rat was divided in three groups: control, daidzein (150 mg/kg diet), and estradiol (4.2 μg/rat/day) groups. In both CCK1R+ and CCK1R- rats, daidzein feeding and estradiol treatment significantly decreased food intake. Daidzein feeding significantly reduced food intake in ovariectomized ObRb- rats, although not in ObRb+ rats. Estradiol treatment significantly lowered food intake in ovariectomized ObRb+ and ObRb- rats. In the ovariectomized rats, estradiol treatment significantly increases uterine weight, while daidzein feeding did not change it, suggesting that daidzein might have no or weak estrogenic effect in our experiment. These results suggest that CCK1R and ObRb signalings were not essential for the daidzein- and estradiol-induced anorectic action.
Kirchner, Henriette; Heppner, Kristy M; Holland, Jenna; Kabra, Dhiraj; Tschöp, Matthias H; Pfluger, Paul T
2013-01-01
Type 2 Diabetes is a global health burden and based on current estimates will become an even larger problem in the future. Developing new strategies to prevent and treat diabetes is a scientific challenge of high priority. The stomach hormone ghrelin has been associated with playing a role in the regulation of glucose homeostasis. However, its precise mechanism and impact on whole glucose metabolism remains to be elucidated. This study aims to clarify the role of the two ghrelin isoforms acyl- and desacyl ghrelin in regulating glucose homeostasis. Therefore ghrelin activating enzyme Ghrelin-O-acyltransferase (GOAT) was ablated in leptin-deficient ob/ob mice to study whether specific acyl ghrelin deficiency or desacyl ghrelin abundance modifies glucose tolerance on a massively obese background. As targeted deletion of acyl ghrelin does not improve glucose homeostasis in our GOAT-ob/ob mouse model we conclude that neither acyl ghrelin nor the increased ratio of desacyl/acyl ghrelin is crucial for controlling glucose homeostasis in the here presented model of massive obesity induced by leptin deficiency.
McDonald, Michelle L; Huang, Andy; Proudfoot, James A; Le, Joan T; Chiang, George J; Bush, Ruth A
2016-01-01
Evaluate the relationship between body mass index (BMI), overweight status (OW), or obesity (OB) and ambulatory status in a predominantly Hispanic population of children with spinal dysraphism (SD). Retrospective data were extracted from records of 272 children and youth aged 0-24 years with a diagnosis of SD. Body mass index (BMI) and OW / OB rates were calculated for children 0-3 years, 4-11 years, and adolescents older than 11. Ethnicity was predominantly Hispanic (65.4%). No difference in mean BMI or OW / OB rate was found between ambulation groups (p = .20; p = .72). Mean BMI and OW / OB rate increased with increasing age in all groups (p < .001; p = .02). Forty-four percent of patients were OW / OB, which was greater among Hispanics (48.2%) compared with non-Hispanics [(35.2%), p = .03]. Female gender was a risk factor for increased BMI among Hispanics (p = .00). Despite no difference in ambulatory status, increasing BMI and OW / OB are associated with Hispanic ethnicity and increasing age.
Obliterative bronchiolitis in fibreglass workers: a new occupational disease?
Cullinan, Paul; McGavin, Clive R; Kreiss, Kathleen; Nicholson, Andrew G; Maher, Toby M; Howell, Tim; Banks, John; Newman Taylor, Anthony J; Chen, Chi-Hsien; Tsai, Perng-Jy; Shih, Tung-Sheng; Burge, P Sherwood
2013-05-01
Obliterative bronchiolitis (OB) is a rare disease with a small number of established occupational aetiologies. We describe a case series of severe OB in workers making glass-reinforced plastics. Workplace exposures were the likely cause after the independent diagnosis of OB in two workers laying up the fibreglass hulls of yachts; the second worker took over the job of the first after he left following a lung transplant. Presentation of these two cases at international meetings led to others identifying similar workers. We identified six workers with good evidence of OB. All were involved in preparing fibreglass with styrene resins, five as boat builders laying up fibreglass hulls and one during cooling-tower fabrication. The disease came on rapidly without unusual acute exposures. Two patients had lung transplants, while another died while waiting for one. Histology confirmed OB in the four with biopsies/post-mortem examinations or explanted lungs. A rare, potentially fatal disease occurring in six workers laying up fibreglass with styrene resins from five different worksites suggests that work exposures were the cause of their OB. The precise agent responsible awaits identification.
Martel, Cecile; Allouche, Maya; Esposti, Davide Degli; Fanelli, Elena; Boursier, Céline; Henry, Céline; Chopineau, Joel; Calamita, Giuseppe; Kroemer, Guido; Lemoine, Antoinette; Brenner, Catherine
2013-01-01
Nonalcoholic steatosis is a liver pathology characterized by fat accumulation and severe metabolic alterations involving early mitochondrial impairment and late hepatocyte cell death. However, mitochondrial dysfunction mechanisms remain elusive. Using four models of nonalcoholic steatosis, i.e., livers from patients with fatty liver disease, ob/ob mice, mice fed a high-fat diet, and in vitro models of lipotoxicity, we show that outer mitochondrial membrane permeability is altered and identified a posttranslational modification of voltage-dependent anion channel (VDAC), a membrane channel and NADH oxidase, as a cause of early mitochondrial dysfunction. Thus, in nonalcoholic steatosis VDAC exhibits reduced threonine phosphorylation, which increases the influx of water and calcium into mitochondria, sensitizes the organelle to matrix swelling, depolarization, and cytochrome c release without inducing cell death. This also amplifies VDAC enzymatic and channel activities regulation by calcium and modifies its interaction with proteic partners. Moreover, lipid accumulation triggers a rapid lack of VDAC phosphorylation by glycogen synthase kinase 3 (GSK3). Pharmacological and genetic manipulations proved GSK3 to be responsible for VDAC phosphorylation in normal cells. Notably, VDAC phosphorylation level correlated with steatosis severity in patients. VDAC acts as an early sensor of lipid toxicity and its GSK3-mediated phosphorylation status controls outer mitochondrial membrane permeabilization in hepatosteatosis. Copyright © 2012 American Association for the Study of Liver Diseases.
On Chorin's Method for Stationary Solutions of the Oberbeck-Boussinesq Equation
NASA Astrophysics Data System (ADS)
Kagei, Yoshiyuki; Nishida, Takaaki
2017-06-01
Stability of stationary solutions of the Oberbeck-Boussinesq system (OB) and the corresponding artificial compressible system is considered. The latter system is obtained by adding the time derivative of the pressure with small parameter ɛ > 0 to the continuity equation of (OB), which was proposed by A. Chorin to find stationary solutions of (OB) numerically. Both systems have the same sets of stationary solutions and the system (OB) is obtained from the artificial compressible one as the limit ɛ \\to 0 which is a singular limit. It is proved that if a stationary solution of the artificial compressible system is stable for sufficiently small ɛ > 0, then it is also stable as a solution of (OB). The converse is proved provided that the velocity field of the stationary solution satisfies some smallness condition.
NASA Astrophysics Data System (ADS)
Lee, G. H.; Park, H. B.
2014-12-01
Acoustic Doppler Current Profiler (ADCP), designed for measuring velocity profile, is now widely used for the estimation of suspended sediment concentration from acoustic backscatter intensity, but its application to estuarine environments has not been vigorously tested. In this study, we examined the inversion capability of two ADCPs with 600 and 1200 kHz at three Korean estuaries: macrotidal Han river estuary (HRE), microtidal Nakdong river estuary (NRE), and anthropogenically altered macrotidal Yeongsan river estuary (YRE). In particular, we examined the relative importance of the sound attenuations due to water (aw) and sediment (as) in response to sediment characteristics (size and concentration) as well as changing salinity and temperature. The inverted concentration was compared with reference concentrations obtained either water samples or Optical Backscatter Sensors. In NRE and YRE, where suspended sediment concentrations were smaller than 0.2 kg/m3, the acoustic inversion performed poorly only with as (R2 = 0.05 and 0.39 for NRE and YRE, respectively), but well with aw (R2 = 0.70 and 0.64 for NRE and YRE, respectively). Thus, it is important to accurately constrain aw in low-concentration estuarine environments. However, we did not find that the varying aw performed considerably better than the constant aw. On the other hand, the acoustic inversion was poorest at HRE regardless of aw and as (R2 = 0.58 and mean relative error =45%). The large discrepancy appears to result from the poorly constrained, spatially and temporally varying sediment characteristics (grain size, density and concentration) due to non-local sediment transport at macrotidal HRE.
NASA Astrophysics Data System (ADS)
Park, Hyo-Bong; Lee, Guan-hong
2016-03-01
Acoustic Doppler Current Profilers (ADCP), designed for measuring velocity profiles, are widely used for the estimation of suspended sediment concentration from acoustic backscatter strength, but its application to estuarine environments requires further refinement. In this study, we examined the inversion capability of two ADCPs with 600 and 1200 kHz in three Korean estuaries: the supra-macrotidal Han River Estuary (HRE), microtidal Nakdong River Estuary (NRE), and anthropogenically altered macrotidal Yeongsan River Estuary (YRE). In particular, we examined the relative importance of the sound attenuations due to water (αw) and sediment (αs) in response to sediment characteristics (size and concentration) as well as changing salinity and temperature. The inverted concentration was compared with reference concentrations obtained either from water samples or Optical Backscatter Sensors. In NRE and YRE, where suspended sediment concentrations were less than 0.2 g/l, the acoustic inversion performed poorly only with αs (r = 0.20 and 0.38 for NRE and YRE, respectively), but well with αw (r = 0.66 and 0.42 for NRE and YRE, respectively). Thus, it is important to accurately constrain αw in low-concentration estuarine environments. However, we did not find that the varying αw performed considerably better than the constant αw. On the other hand, the acoustic inversion was poorest at HRE regardless of αw and αs (r = 0.71 and mean relative error = 45%). The large discrepancy appears to result from the poorly constrained, spatially and temporally varying sediment characteristics (grain size, density and concentration) due to non-local sediment transport in the macrotidal HRE.
NASA Astrophysics Data System (ADS)
Lee, Guan-hong; Park, Hyo-Bong
2015-04-01
Acoustic Doppler Current Profiler (ADCP), designed for measuring velocity profile, is now widely used for the estimation of suspended sediment concentration from acoustic backscatter strength, but its application to estuarine environments has still room for improvement. In this study, we examinedthe inversion capability of two ADCPs with 600 and 1200 kHz at three Korean estuaries: macrotidalHan river estuary (HRE), microtidalNakdong river estuary (NRE), and anthropogenically altered macrotidalYeongsan river estuary (YRE). In particular, we examined the relative importance of the sound attenuations due to water (αw) and sediment (αs) in response to sediment characteristics (size and concentration) as well as changing salinity and temperature. The inverted concentration was compared with reference concentrations obtained either water samples or Optical Backscatter Sensors. In NRE and YRE, where suspended sediment concentrations were smaller than 0.2 g/l, the acoustic inversion performed poorly only with αs (r = 0.20and 0.38for NRE and YRE, respectively), but well with αw (r = 0.66and 0.42 for NREand YRE, respectively). Thus, it is important to accurately constrain αw in low-concentration estuarine environments. However, we did not find that the varying αw performed considerably better than the constant αw. On the other hand, the acoustic inversion was poorest at HRE regardless of αw and αs (r = 0.71 and mean relative error =45%). The large discrepancy appears to result from the poorly constrained, spatially and temporally varying sediment characteristics (grain size, density and concentration) due to non-local sediment transport at macrotidal HRE.
Oil detection in a coastal marsh with polarimetric Synthetic Aperture Radar (SAR)
Ramsey, Elijah W.; Rangoonwala, Amina; Suzuoki, Yukihiro; Jones, Cathleen E.
2011-01-01
The National Aeronautics and Space Administration's airborne Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) was deployed in June 2010 in response to the Deepwater Horizon oil spill in the Gulf of Mexico. UAVSAR is a fully polarimetric L-band Synthetic Aperture Radar (SAR) sensor for obtaining data at high spatial resolutions. Starting a month prior to the UAVSAR collections, visual observations confirmed oil impacts along shorelines within northeastern Barataria Bay waters in eastern coastal Louisiana. UAVSAR data along several flight lines over Barataria Bay were collected on 23 June 2010, including the repeat flight line for which data were collected in June 2009. Our analysis of calibrated single-look complex data for these flight lines shows that structural damage of shoreline marsh accompanied by oil occurrence manifested as anomalous features not evident in pre-spill data. Freeman-Durden (FD) and Cloude-Pottier (CP) decompositions of the polarimetric data and Wishart classifications seeded with the FD and CP classes also highlighted these nearshore features as a change in dominant scattering mechanism. All decompositions and classifications also identify a class of interior marshes that reproduce the spatially extensive changes in backscatter indicated by the pre- and post-spill comparison of multi-polarization radar backscatter data. FD and CP decompositions reveal that those changes indicate a transform of dominant scatter from primarily surface or volumetric to double or even bounce. Given supportive evidence that oil-polluted waters penetrated into the interior marshes, it is reasonable that these backscatter changes correspond with oil exposure; however, multiple factors prevent unambiguous determination of whether UAVSAR detected oil in interior marshes.
Gogiashvili, Mikheil; Edlund, Karolina; Gianmoena, Kathrin; Marchan, Rosemarie; Brik, Alexander; Andersson, Jan T; Lambert, Jörg; Madjar, Katrin; Hellwig, Birte; Rahnenführer, Jörg; Hengstler, Jan G; Hergenröder, Roland; Cadenas, Cristina
2017-02-01
Metabolic perturbations resulting from excessive hepatic fat accumulation are poorly understood. Thus, in this study, leptin-deficient ob/ob mice, a mouse model of fatty liver disease, were used to investigate metabolic alterations in more detail. Metabolites were quantified in intact liver tissues of ob/ob (n = 8) and control (n = 8) mice using high-resolution magic angle spinning (HR-MAS) 1 H-NMR. In addition, after demonstrating that HR-MAS 1 H-NMR does not affect RNA integrity, transcriptional changes were measured by quantitative real-time PCR on RNA extracted from the same specimens after HR-MAS 1 H-NMR measurements. Importantly, the gene expression changes obtained agreed with those observed by Affymetrix microarray analysis performed on RNA isolated directly from fresh-frozen tissue. In total, 40 metabolites could be assigned in the spectra and subsequently quantified. Quantification of lactate was also possible after applying a lactate-editing pulse sequence that suppresses the lipid signal, which superimposes the lactate methyl resonance at 1.3 ppm. Significant differences were detected for creatinine, glutamate, glycine, glycolate, trimethylamine-N-oxide, dimethylglycine, ADP, AMP, betaine, phenylalanine, and uridine. Furthermore, alterations in one-carbon metabolism, supported by both metabolic and transcriptional changes, were observed. These included reduced demethylation of betaine to dimethylglycine and the reduced expression of genes coding for transsulfuration pathway enzymes, which appears to preserve methionine levels, but may limit glutathione synthesis. Overall, the combined approach is advantageous as it identifies changes not only at the single gene or metabolite level but also deregulated pathways, thus providing critical insight into changes accompanying fatty liver disease. Graphical abstract A Evaluation of RNA integrity before and after HR-MAS 1 H-NMR of intact mouse liver tissue. B Metabolite concentrations and gene expression levels assessed in ob/ob (steatotic) and ob/+ (control) mice using HR-MAS 1 H-NMR and qRT-PCR, respectively.
Beck, George R; Khazai, Natasha B; Bouloux, Gary F; Camalier, Corinne E; Lin, Yiming; Garneys, Laura M; Siqueira, Joselita; Peng, Limin; Pasquel, Francisco; Umpierrez, Denise; Smiley, Dawn; Umpierrez, Guillermo E
2013-03-01
Thiazolidinedione (TZD) therapy has been associated with an increased risk of bone fractures. Studies in rodents have led to a model in which decreased bone quality in response to TZDs is due to a competition of lineage commitment between osteoblasts (OBs) and adipocytes (ADs) for a common precursor cell, resulting in decreased OB numbers. Our goal was to investigate the effects of TZD exposure on OB-AD lineage determination from primary human bone marrow stromal cells (hBMSCs) both in vitro and in vivo from nondiabetic subjects and patients with type 2 diabetics. Our experimental design included 2 phases. Phase 1 was an in vitro study of TZD effects on the differentiation of hBMSCs into OBs and ADs in nondiabetic subjects. Phase 2 was a randomized, placebo-controlled trial to determine the effects of 6-month pioglitazone treatment in vivo on hBMSC differentiation using AD/OB colony forming unit assays in patients with type 2 diabetes. In vitro, TZDs (pioglitazone and rosiglitazone) enhanced the adipogenesis of hBMSCs, whereas neither altered OB differentiation or function as measured by alkaline phosphatase activity, gene expression, and mineralization. The ability of TZDs to enhance adipogenesis occurred at a specific time/stage of the differentiation process, and pretreating with TZDs did not further enhance adipogenesis. In vivo, 6-month TZD treatment decreased OB precursors, increased AD precursors, and increased total colony number in patients with type 2 diabetes. Our results indicate that TZD exposure in vitro potently stimulates adipogenesis but does not directly alter OB differentiation/mineralization or lineage commitment from hBMSCs. However, TZD treatment in type 2 diabetic patients results in decreased osteoblastogenesis from hBMSCs compared with placebo, indicating an indirect negative effect on OBs and suggesting an alternative model by which TZDs might negatively regulate bone quality. Copyright © 2013 Mosby, Inc. All rights reserved.
Peritumoral adipose tissue as a source of inflammatory and angiogenic factors in colorectal cancer.
Amor, S; Iglesias-de la Cruz, M C; Ferrero, E; García-Villar, O; Barrios, V; Fernandez, N; Monge, L; García-Villalón, A L; Granado, M
2016-02-01
Obesity is a risk factor for the development of human colorectal cancer (CC). The aim of this work is to report the inflammatory and angiogenic scenario in lean (BMI < 25 kg/m2) and obese (BMI > 30 kg/m2) patients with and without CC and to assess the role of peritumoral adipose tissue in CC-induced inflammation. Patients were divided in four experimental groups: obese patients with CC (OB-CC), lean patients with CC (LEAN-CC), obese patients without CC (OB), and lean patients without CC (LEAN). Plasma levels of pro-inflammatory cytokines (interleukin (IL)-6, IL-4, IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF) were increased in OB-CC patients. Peritumoral adipose tissue (TF) explants and cultured mature adipocytes secreted higher amounts of nitrites and nitrates than did control and non-tumoral (NTF) adipose tissue both alone and in response to lipopolysaccharide (LPS). Nitrite and nitrate secretion was also increased in TF explants from OB-CC patients compared with that from LEAN-CC patients. Gene expression of adiponectin, tumor necrosis factor alpha (TNF-α), insulin-like growth factor type I (IGF-I), cyclooxygenase-2 (COX-2), and peroxisome proliferator-activated receptor γ (PPAR-γ) was increased in TF explants from CC patients. LPS increased the gene expression of IL-6, IL-10, TNF-α, vascular endothelial growth factor (VEGF), and COX-2 in OB and in TF explants from OB-CC patients. COX-2 and PPAR-γ inhibition further increased LPS-induced release of nitrites and nitrates in TF explants and adipocytes from OB-CC patients. In conclusion, OB-CC patients have increased plasma levels of pro-inflammatory and angiogenic factors. TF from OB-CC patients shows an increased secretion of inflammatory markers compared with both TF from LEAN-CC and non-tumoral adipose tissue (AT) through a COX-2- and PPAR-γ-independent mechanism.
The Stem Cell Marker Lgr5 Defines a Subset of Postmitotic Neurons in the Olfactory Bulb.
Yu, Yiqun; Moberly, Andrew H; Bhattarai, Janardhan P; Duan, Chen; Zheng, Qian; Li, Fangqi; Huang, Hugh; Olson, William; Luo, Wenqin; Wen, Tieqiao; Yu, Hongmeng; Ma, Minghong
2017-09-27
Lgr5, leucine-rich repeat-containing G-protein coupled receptor 5, is a bona fide biomarker for stem cells in multiple tissues. Lgr5 is also expressed in the brain, but the identities and properties of these Lgr5 + cells are still elusive. Using an Lgr5-EGFP reporter mouse line, we found that, from early development to adulthood, Lgr5 is highly expressed in the olfactory bulb (OB), an area with ongoing neurogenesis. Immunostaining with stem cell, glial, and neuronal markers reveals that Lgr5 does not label stem cells in the OB but instead labels a heterogeneous population of neurons with preference in certain subtypes. Patch-clamp recordings in OB slices reveal that Lgr5-EGFP + cells fire action potentials and display spontaneous excitatory postsynaptic events, indicating that these neurons are integrated into OB circuits. Interestingly, R-spondin 3, a potential ligand of Lgr5, is also expressed in the adult OB. Collectively, our data indicate that Lgr5-expressing cells in the OB are fully differentiated neurons and imply distinct roles of Lgr5 and its ligand in postmitotic cells. SIGNIFICANCE STATEMENT Lgr5 (leucine-rich repeat-containing G-protein coupled receptor 5) is a bona fide stem cell marker in many body organs. Here we report that Lgr5 is also highly expressed in the olfactory bulb (OB), the first relay station in the brain for processing odor information and one of the few neural structures that undergo continuous neurogenesis. Surprisingly, Lgr5 is not expressed in the OB stem cells, but instead in a few subtypes of terminally differentiated neurons, which are incorporated into the OB circuit. This study reveals that Lgr5 + cells in the brain represent a nonstem cell lineage, implying distinct roles of Lgr5 in postmitotic neurons. Copyright © 2017 the authors 0270-6474/17/379403-12$15.00/0.
Bernal, Alexandra; Simón, Oihane; Williams, Trevor; Muñoz, Delia
2013-01-01
A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus wild-type isolate from the Canary Islands, Spain, named ChchSNPV-TF1 (ChchTF1-wt), appears to have great potential as the basis for a biological insecticide for control of the pest. An improved understanding of the genotypic structure of this wild-type strain population should facilitate the selection of genotypes for inclusion in a bioinsecticidal product. Eight genetically distinct genotypes were cloned in vitro: ChchTF1-A to ChchTF1-H. Quantitative real-time PCR (qPCR) analysis confirmed that ChchTF1-A accounted for 36% of the genotypes in the wild-type population. In bioassays, ChchTF1-wt occlusion bodies (OBs) were significantly more pathogenic than any of the component single-genotype OBs, indicating that genotype interactions were likely responsible for the pathogenicity phenotype of wild-type OBs. However, the wild-type population was slower killing and produced higher OB yields than any of the single genotypes alone. These results strongly suggested that the ChchTF1-wt population is structured to maximize its transmission efficiency. Experimental OB mixtures and cooccluded genotype mixtures containing the most abundant and the rarest genotypes, at frequencies similar to those at which they were isolated, revealed a mutualistic interaction that restored the pathogenicity of OBs. In OB and cooccluded mixtures containing only the most abundant genotypes, ChchTF1-ABC, OB pathogenicity was even greater than that of wild-type OBs. The ChchTF1-ABC cooccluded mixture killed larvae 33 h faster than the wild-type population and remained genotypically and biologically stable throughout five successive passages in vivo. In conclusion, the ChchTF1-ABC mixture shows great potential as the active ingredient of a bioinsecticide to control C. chalcites in the Canary Islands. PMID:24096419
Barrera, Gloria; Williams, Trevor; Villamizar, Laura; Caballero, Primitivo; Simón, Oihane
2013-01-01
A Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different genotypic variants, 83 plaque purified virus were characterized. Ten distinct genotypes were identified (named A through J). SfCOL-A was the most prevalent (71±2%; mean ± SE) showing a PstI restriction profile indistinguishable to that of SfCOL-wt. The remaining nine genotypes presented genomic deletions of 3.8 - 21.8 Kb located mainly between nucleotides 11,436 and 33,883 in the reference genome SfMNPV-B, affecting the region between open reading frames (ORFs) sf20 and sf33. The insecticidal activity of each genotype from SfCOL-wt and several mixtures of genotypes was compared to that of SfCOL-wt. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of genotype mixtures co-occluded within OBs were consistently reduced in two-genotype mixtures involving equal proportions of SfCOL-A and one of three deletion genotypes (SfCOL-C, -D or -F). Speed of kill and OB production were improved only when the certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any of the component genotypes, or mixtures thereof. Deleted genotypes reduced OB potency but increased OB production of the SfCOL-wt population, which is structured to maximize the production of OBs in each infected host. PMID:24116220
Sniffin' Sticks and olfactory system imaging in patients with Kallmann syndrome.
Ottaviano, Giancarlo; Cantone, Elena; D'Errico, Arianna; Salvalaggio, Alessandro; Citton, Valentina; Scarpa, Bruno; Favaro, Angela; Sinisi, Antonio Agostino; Liuzzi, Raffaele; Bonanni, Guglielmo; Di Salle, Francesco; Elefante, Andrea; Manara, Renzo; Staffieri, Alberto; Martini, Alessandro; Brunetti, Arturo
2015-09-01
The relationship between olfactory function, rhinencephalon and forebrain changes in Kallmann syndrome (KS) have not been adequately investigated. We evaluated a large cohort of male KS patients using Sniffin' Sticks and MRI in order to study olfactory bulb (OB) volume, olfactory sulcus (OS) depth, cortical thickness close to the OS, and olfactory phenotype. Olfaction was assessed administering Sniffin' Sticks®, in 38 KS patients and 17 controls (by means of Screening 12 test®). All subjects underwent magnetic resonance imaging (MRI) to study OB volume, sulcus depth, and cortical thickness. Compared to controls, KS patients showed smaller OB volume (p<0.0001), reduced sulcus depth (p<0.0001), and thicker cortex in the region close to the OS (p<0.0001). Anosmic KS patients had smaller OB than controls and hyposmic KS patients; there was no difference between hyposmic KS patients and controls. OB volume correlated with Sniffin' Sticks score (r = 0.64; p < 0.001), OS depth (p<0.0001) and, inversely, with cortical thickness changes (p<0.0001). Sniffin' Sticks showed an inverse correlation with cortical thickness (r = -0.5; p<0.0001) and a trend toward a statistically significant correlation with OS depth. The present study provides further evidence of the strict relationship between olfaction and OB volume. The strong correlation between OB volume and the overlying cortical changes highlights the key role of rhinencephalon in forebrain embryogenesis. © 2015 ARS-AAOA, LLC.
Kiviranta, Riku; Yamana, Kei; Saito, Hiroaki; Ho, Daniel K.; Laine, Julius; Tarkkonen, Kati; Nieminen-Pihala, Vappu; Hesse, Eric; Correa, Diego; Määttä, Jorma; Tessarollo, Lino; Rosen, Evan D.; Horne, William C.; Jenkins, Nancy A.; Copeland, Neal G.; Warming, Soren
2013-01-01
Bone homeostasis is maintained by the coupled actions of hematopoietic bone-resorbing osteoclasts (OCs) and mesenchymal bone-forming osteoblasts (OBs). Here we identify early B cell factor 1 (Ebf1) and the transcriptional coregulator Zfp521 as components of the machinery that regulates bone homeostasis through coordinated effects in both lineages. Deletion of Zfp521 in OBs led to impaired bone formation and increased OB-dependent osteoclastogenesis (OC-genesis), and deletion in hematopoietic cells revealed a strong cell-autonomous role for Zfp521 in OC progenitors. In adult mice, the effects of Zfp521 were largely caused by repression of Ebf1, and the bone phenotype of Zfp521+/− mice was rescued in Zfp521+/−:Ebf1+/− mice. Zfp521 interacted with Ebf1 and repressed its transcriptional activity. Accordingly, deletion of Zfp521 led to increased Ebf1 activity in OBs and OCs. In vivo, Ebf1 overexpression in OBs resulted in suppressed bone formation, similar to the phenotype seen after OB-targeted deletion of Zfp521. Conversely, Ebf1 deletion led to cell-autonomous defects in both OB-dependent and cell-intrinsic OC-genesis, a phenotype opposite to that of the Zfp521 knockout. Thus, we have identified the interplay between Zfp521 and Ebf1 as a novel rheostat for bone homeostasis. PMID:23569325
Emiroğlu, Mustafa; Sert, İsmail; İnal, Abdullah; Karaali, Cem; Peker, Kemal; İlhan, Enver; Gülcelik, Mehmet; Erol, Varlık; Güngör, Hilmi; Can, Didem; Aydın, Cengiz
2014-12-01
Oncoplastic Breast Surgery (OBS), which is a combination of oncological procedures and plastic surgery techniques, has recently gained widespread use. To assess the experiences, practice patterns and preferred approaches to Oncoplastic and Reconstructive Breast Surgery (ORBS) undertaken by general surgeons specializing in breast surgery in Turkey. Cross-sectional study. Between December 2013 and February 2014, an eleven-question survey was distributed among 208 general surgeons specializing in breast surgery. The questions focused on the attitudes of general surgeons toward performing oncoplastic breast surgery (OBS), the role of the general surgeon in OBS and their training for it as well as their approaches to evaluating cosmetic outcomes in Breast Conserving Surgery (BCS) and informing patients about ORBS preoperatively. Responses from all 208 surgeons indicated that 79.8% evaluated the cosmetic outcomes of BCS, while 94.2% informed their patients preoperatively about ORBS. 52.5% performed BCS (31.3% themselves, 21.1% together with a plastic surgeon). 53.8% emphasized that general surgeons should carry out OBS themselves. 36.1% of respondents suggested that OBS training should be included within mainstream surgical training, whereas 27.4% believed this training should be conducted by specialised centres. Although OBS procedure rates are low in Turkey, it is encouraging to see general surgeons practicing ORBS themselves. The survey demonstrates that our general surgeons aspire to learn and utilize OBS techniques.
Yokosuka, Makoto; Hagiwara, Akiko; Saito, Toru R; Tsukahara, Naoki; Aoyama, Masato; Wakabayashi, Yoshihiro; Sugita, Shoei; Ichikawa, Masumi
2009-09-01
The nasal cavity and olfactory bulb (OB) of the Japanese jungle crow (Corvus macrorhynchos) were studied using computed tomography (CT) and histochemical staining. The nasal septum divided the nasal cavity in half. The anterior and maxillary conchae were present on both sides of the nasal cavity, but the posterior concha was indistinct. A small OB was present on the ventral surface of the periphery of the cerebrum. The OB-brain ratio--the ratio of the size of the OB to that of the cerebral hemisphere--was 6.13. The olfactory nerve bundles projected independently to the OB, which appeared fused on gross examination. Histochemical analysis confirmed the fusion of all OB layers. Using a neural tracer, we found that the olfactory nerve bundles independently projected to the olfactory nerve layer (ONL) and glomerular layer (GL) of the left and right halves of the fused OB. Only 4 of 21 lectins bound to the ONL and GL. Thus, compared with mammals and other birds, the jungle crow may have a poorly developed olfactory system and an inferior sense of olfaction. However, it has been contended recently that the olfactory abilities of birds cannot be judged from anatomical findings alone. Our results indicate that the olfactory system of the jungle crow is an interesting research model to evaluate the development and functions of vertebrate olfactory systems.
Hirose, Noritaka; Saitoh, Akiyoshi; Kamei, Junzo
2016-10-01
Olfactory bulbectomized (OB) mice produce agitated anxiety-like behaviors in the hole-board test, which was expressed by an increase in head-dipping counts and a decrease in head-dipping latencies. However, the associated mechanisms remain unclear. In the present study, MK-801 (10, 100μg/kg), a selective N-methyl-d-aspartate (NMDA) receptor antagonist, significantly and dose-dependently suppressed the increased head-dipping behaviors in OB mice, without affecting sham mice. Similar results were obtained with another selective NMDA receptor antagonist D-AP5 treatment in OB mice. On the other hand, muscimol, a selective aminobutyric acid type A (GABAA) receptor agonist produced no effects on these hyperemotional behaviors in OB mice at a dose (100μg/kg) that produced anxiolytic-like effects in sham mice. Interestingly, glutamine contents and glutamine/glutamate ratios were significantly increased in the amygdala and frontal cortex of OB mice compared to sham mice. Based on these results, we concluded that the glutamatergic NMDA receptors are involved in the expression of increased head-dipping behaviors in the hole-board tests of OB mice. Accordingly, the changes in glutamatergic transmission in frontal cortex and amygdala may play important roles in the expression of these abnormal behaviors in OB mice. Copyright © 2016. Published by Elsevier B.V.
The antidepressant-like effect of Ocimum basilicum in an animal model of depression.
Ali, S S; Abd El Wahab, M G; Ayuob, N N; Suliaman, M
2017-01-01
We investigated the efficacy of Ocimum basilicum (OB) essential oils for treating depression related behavioral, biochemical and histopathological changes caused by exposure to chronic unpredictable mild stress (CUMS) in mice and to explore the mechanism underlying the pathology. Male albino mice were divided into four groups: controls; CUMS; CUMS plus fluoxetine, the antidepressant administered for pharmacological validation of OB; and CUMS plus OB. Behavioral tests included the forced swim test (FST), elevated plus-maze (EPM) and the open field test (OFT); these tests were performed at the end of the experiment. We assessed serum corticosterone level, protein, gene and immunoexpression of brain-derived neurotropic factor (BDNF) and glucocorticoid receptors (GRs) as well as immunoexpression of glial fibrillary acidic protein (GFAP), Ki67, caspase-3 in the hippocampus. CUMS caused depression in the mice as evidenced by prolonged immobility in the FST, prolonged time spent in the open arms during the EPM test and reduction of open field activity in the OFT. OB ameliorated the CUMS induced depressive status. OB significantly reduced the corticosterone level and up-regulated protein and gene expressions of BDNF and GR. OB reduced CUMS induced hippocampal neuron atrophy and apoptosis, and increased the number of the astrocytes and new nerve cells. OB significantly increased GFAP-positive cells as well as BDNF and GR immunoexpression in the hippocampus.
Misra, Rajesh Chandra; Sandeep; Kamthan, Mohan; Kumar, Santosh; Ghosh, Sumit
2016-01-01
Plant often responds to fungal pathogens by expressing a group of proteins known as pathogenesis-related proteins (PRs). The expression of PR is mediated through pathogen-induced signal-transduction pathways that are fine-tuned by phytohormones such as methyl jasmonate (MeJA). Here, we report functional characterization of an Ocimum basilicum PR5 family member (ObTLP1) that was identified from a MeJA-responsive expression sequence tag collection. ObTLP1 encodes a 226 amino acid polypeptide that showed sequence and structural similarities with a sweet-tasting protein thaumatin of Thaumatococcus danielli and also with a stress-responsive protein osmotin of Nicotiana tabacum. The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition, and responsive to biotic and abiotic stresses, and multiple phytohormone elicitations. Bacterially-expressed recombinant ObTLP1 inhibited mycelial growth of the phytopathogenic fungi, Scleretonia sclerotiorum and Botrytis cinerea; thereby, suggesting its antifungal activity. Ectopic expression of ObTLP1 in Arabidopsis led to enhanced tolerance to S. sclerotiorum and B. cinerea infections, and also to dehydration and salt stress. Moreover, induced expression of the defense marker genes suggested up-regulation of the defense-response pathways in ObTLP1-expressing Arabidopsis upon fungal challenge. Thus, ObTLP1 might be useful for providing tolerance to the fungal pathogens and abiotic stresses in crops. PMID:27150014
Misra, Rajesh Chandra; Sandeep; Kamthan, Mohan; Kumar, Santosh; Ghosh, Sumit
2016-05-06
Plant often responds to fungal pathogens by expressing a group of proteins known as pathogenesis-related proteins (PRs). The expression of PR is mediated through pathogen-induced signal-transduction pathways that are fine-tuned by phytohormones such as methyl jasmonate (MeJA). Here, we report functional characterization of an Ocimum basilicum PR5 family member (ObTLP1) that was identified from a MeJA-responsive expression sequence tag collection. ObTLP1 encodes a 226 amino acid polypeptide that showed sequence and structural similarities with a sweet-tasting protein thaumatin of Thaumatococcus danielli and also with a stress-responsive protein osmotin of Nicotiana tabacum. The expression of ObTLP1 in O. basilicum was found to be organ-preferential under unstressed condition, and responsive to biotic and abiotic stresses, and multiple phytohormone elicitations. Bacterially-expressed recombinant ObTLP1 inhibited mycelial growth of the phytopathogenic fungi, Scleretonia sclerotiorum and Botrytis cinerea; thereby, suggesting its antifungal activity. Ectopic expression of ObTLP1 in Arabidopsis led to enhanced tolerance to S. sclerotiorum and B. cinerea infections, and also to dehydration and salt stress. Moreover, induced expression of the defense marker genes suggested up-regulation of the defense-response pathways in ObTLP1-expressing Arabidopsis upon fungal challenge. Thus, ObTLP1 might be useful for providing tolerance to the fungal pathogens and abiotic stresses in crops.
Majeed, Farrukh; Yar, Talay; Alsunni, Ahmed; Alhawaj, Ali Fouad; AlRahim, Ahmed; Alzaki, Muneer
2017-01-01
Obesity and caffeine consumption may lead to autonomic disturbances that can result in a wide range of cardiovascular disorders. To determine autonomic disturbances produced by the synergistic effects of overweight or obesity (OW/OB) and energy drinks. Cross-sectional, analytical. Physiology department at a university in Saudi Arabia. University students, 18-22 years of age, of normal weight (NW) and OW/OB were recruited by convenience sampling. Autonomic testing by the Valsalva ratio (VR) along with systolic and diastolic blood pressure, pulse pressure, and mean arterial blood pressure were measured at baseline (0 minute) and 60 minutes after energy drink consumption. Autonomic disturbance, hemodynamic changes. In 50 (27 males and 23 females) subjects, 21 NW and 29 OW/OB, a significant decrease in VR was observed in OW/OB subjects and in NW and OW/OB females at 60 minutes after energy drink consumption. Values of systolic and diastolic blood pressure, pulse pressure and mean arterial blood pressure were also significantly higher in OW/OB and in females as compared to NW and males. BMI was negatively correlated with VR and diastolic blood pressure at 60 minutes. Obesity and energy drinks alter autonomic functions. In some individuals, OW/OB may augment these effects. Due to time and resource restraints, only the acute effects of energy drinks were examined.
2009-03-01
Framework ( IATF ) as their guidance for information assurance. The IATF defines what high 5 OB1 is compatible with and intends to use legacy...standardized levels of security. The OB1 team using the IATF as guidance defines high robustness as proving to the maximum extent possible, that
Flooding of the Ob and Irtysh Rivers, Russia
NASA Technical Reports Server (NTRS)
2002-01-01
This pair of true- and false-color images shows flooding along the Ob' (large east-west running river) and Irtysh (southern tributary of the Ob') on July 7, 2002. In the false-color image, land surfaces are orange-gold and flood waters are black or dark blue. Fires are marked with red dots in both images. Rivers
Leinung, Matthew C; Grasso, Patricia
2012-11-10
The escalation predicted for the incidence of both type 2 diabetes mellitus and obesity has prompted investigators to search for additional pharmacotherapeutic approaches to their treatment. Two of these approaches, combination pharmacotherapy and utilization of leptin-related bioactive synthetic peptides as anti-diabetes/anti-obesity agents, were used in the present study. Exenatide or pramlintide acetate was reconstituted in dodecyl maltoside (DDM) in the absence or presence of [D-Leu-4]-OB3, and delivered orally by gavage to insulin-resistant male C57BLK/6-m db/db mice twice daily for 14 days. Body weight gain, food and water intake, blood glucose, and serum insulin levels were measured. Mice given DDM alone for 14 days were 19.7% heavier than they were at the beginning of the study, while oral delivery of exenatide or [D-Leu-4]-OB3 in DDM reduced body weight gain to only 13.9% and 11.5%, respectively, of initial body weight. Mice receiving exenatide and [D-Leu-4]-OB3 were 4.2% lighter than they were at the beginning of the study. In another study, Intravail® treated control mice gained 38.2% of their initial body weight, while mice receiving pramlintide acetate or [D-Leu-4]-OB3 were only 26.8% and 25.4% heavier, respectively, at the end of the study, Co-administration of pramlintide acetate and [D-Leu-4]-OB3 did not further enhance the effect of pramlintide acetate on body weight gain. Food intake was reduced by exenatide, pramlintide acetate, and [D-Leu-4]-OB3 alone, and co-delivery with [D-Leu-4]-OB3 did not induce a further decrease. Water intake was not affected by exenatide, pramlintide acetate, or [D-Leu-4]-OB3 alone, but co-delivery of exenatide or pramlintide acetate with [D-Leu-4]-OB3 resulted in a significant reduction in water intake. Oral delivery of exenatide or pramlintide acetate in DDM significantly lowered blood glucose levels by 20.4% and 30.2%, respectively. Co-delivery with [D-Leu-4]-OB3 further reduced blood glucose by 38.3% and 50.5%, respectively. A concentration-dependent increase in serum insulin was observed in response to increasing concentrations of exenatide, and [D-Leu-4]-OB3 slightly reduced the insulin response to exenatide at all concentrations tested. Increasing concentrations of pramlintide acetate alone did not elevate serum insulin, and when given in combination with [D-Leu-4]-OB3, serum insulin levels fell below those of DDM-treated control mice. Our data indicate that (1) exenatide and pramlintide acetate, currently administered by subcutaneous injection, can be given orally in DDM; (2) the bioactivity of exenatide and pramlintide acetate is retained following oral delivery in DDM; and (3) the effects of exenatide and pramlintide acetate on energy balance and glycemic control can be enhanced by co-administration with [D-Leu-4]-OB3, a synthetic peptide amide with leptin-like activity. Copyright © 2012 Elsevier B.V. All rights reserved.
Donepudi, Ajay C.; Cheng, Qiuqiong; Lu, Zhenqiang James; Cherrington, Nathan J.
2016-01-01
Metabolic syndrome is a multifactorial disease associated with obesity, insulin resistance, diabetes, and the alteration of multiple metabolic hormones. Obesity rates have been rising worldwide, which increases our need to understand how this population will respond to drugs and exposure to other chemicals. The purpose of this study was to determine in lean and obese mice the ontogeny of clinical biomarkers such as serum hormone and blood glucose levels as well as the physiologic markers that correlate with nuclear receptor– and transporter-related pathways. Livers from male and female wild-type (WT) (C57BL/6) and ob/ob mice littermates were collected before, during, and after the onset of obesity. Serum hormone and mRNA levels were analyzed. Physiologic changes and gene expression during maturation and progression to obesity were performed and correlation analysis was performed using canonical correlations. Significant ontogenic changes in both WT and ob/ob mice were observed and these ontogenic changes differ in ob/ob mice with the development of obesity. In males and females, the ontogenic pattern of the expression of genes such as Abcc3, 4, Abcg2, Cyp2b10, and 4a14 started to differ from week 3, and became significant at weeks 4 and 8 in ob/ob mice compared with WT mice. In obese males, serum resistin, glucagon, and glucose levels correlated with the expression of most hepatic ATP-binding cassette (Abc) transporters, whereas in obese females, serum glucagon-like peptide 1 levels were correlated with most hepatic uptake transporters and P450 enzymes. Overall, the correlation between physiologic changes and gene expression indicate that metabolism-related hormones may play a role in regulating the genes involved in drug metabolism and transport. PMID:26847773
do Carmo, Jussara M.; Hosler, Jonathan P.; Hall, John E.
2015-01-01
We investigated obesity-induced changes in kidney lipid accumulation, mitochondrial function, and endoplasmic reticulum (ER) stress in the absence of hypertension, and the potential role of leptin in modulating these changes. We compared two normotensive genetic mouse models of obesity, leptin-deficient ob/ob mice and hyperleptinemic melanocortin-4 receptor-deficient mice (LoxTB MC4R−/−), with their respective lean controls. Compared with controls, ob/ob and LoxTB MC4R−/− mice exhibit significant albuminuria, increased creatinine clearance, and high renal triglyceride content. Renal ATP levels were decreased in both obesity models, and mitochondria isolated from both models showed alterations that would lower mitochondrial ATP production. Mitochondria from hyperleptinemic LoxTB MC4R−/− mice kidneys respired NADH-generating substrates (including palmitate) at lower rates due to an apparent decrease in complex I activity, and these mitochondria showed oxidative damage. Kidney mitochondria of leptin-deficient ob/ob mice showed normal rates of respiration with no evidence of oxidative damage, but electron transfer was partially uncoupled from ATP synthesis. A fourfold induction of C/EBP homologous protein (CHOP) expression indicated induction of ER stress in kidneys of hyperleptinemic LoxTB MC4R−/− mice. In contrast, ER stress was not induced in kidneys of leptin-deficient ob/ob mice. Our findings show that obesity, in the absence of hypertension, is associated with renal dysfunction in mice but not with major renal injury. Alterations to mitochondria that lower cellular ATP levels may be involved in obesity-induced renal injury. The type and severity of mitochondrial and ER dysfunction differs depending upon the presence or absence of leptin. PMID:26290368
Persson-Sjögren, Solveig; Lindström, Per
2004-11-01
Decreased beta-cell function is often observed in older individuals and may predispose to the development of type 2 diabetes. We have studied the age-related effects of M-receptor agonism on insulin release in islets isolated from female ob/ ob and lean mice. Islets were challenged with 11.1 or 16.7 mmol/L glucose in media with HCO3/CO2 (KRBH) or without (KRH). Acetylcholine (ACh) (10 micromol/L) increased glucose-induced insulin release in islets from 4- to 5-week-old ob/ob mice both in KRBH and KRH. In islets from 9- to 13-month-old ob/ob mice, 10 micromol/L ACh and 10 micromol/L carbachol enhanced insulin release in KRBH but not in KRH. ACh increased insulin release in islets from 4- to 5-week-old and 16-month-old lean mice incubated in KRH but not in islets from 24-month-old lean mice. The Na/H exchange inhibitor dimethylamiloride (100 micromol/L) did not affect insulin release stimulated by M-receptor agonists. Carbachol did not enhance glucose-induced insulin secretion in islets from 9- to 10-month-old ob/ob mice in the presence of low extracellular Na concentration. ACh stimulated cytoplasmic Ca mobilization in islets from 9- to 10-month-old mice also when bicarbonate was omitted. The results suggest that cholinergic signal transduction involving extracellular bicarbonate and Na is reduced with age in mouse pancreatic islets. Chronic hyperglycemia may add to the age-related decrease in M-receptor-mediated insulin release by affecting the buffering capacity of the islets through mechanisms other than amiloride-sensitive proton exchange.
A direct comparison of quality of life in obese and Cushing’s syndrome patients
Abraham, Smita Baid; Abel, Brent S; Rubino, Domenica; Nansel, Tonja; Ramsey, Sheila; Nieman, Lynnette K
2014-01-01
Objective Obese (OB) individuals and patients with Cushing’s syndrome (CS) often have similar clinical presentations. While each group has reduced health-related quality of life (HRQL), it is not known whether the degree of impairment is different and might distinguish between them. The objective of this study was to compare HRQL in these two populations. Design Cross-sectional study. Methods Three hundred and twenty-seven OB patients (48.1±11.7 years; 72.5% women) with weight gain and at least two features of CS were recruited from an outpatient weight management clinic. Sixty-six untreated patients with CS (41.6±13.2 years; 78.8% women) presented to the NIH Clinical Center for evaluation. Subjects completed the SF-36 survey and a locally created symptom questionnaire. Results After adjusting for symptom count, OB patients had a significantly higher (better HRQL) mean physical component summary (PCS) score than CS patients (44.9±0.6 vs 35.4±1.5, P<0.0001). However, the mean mental component summary (MCS) score was lower (worse HRQL) in the OB group (41.6±0.6 vs 50.7±1.6, P<0.0001). Symptom count showed significant correlations with PCS and MCS scores. BMI correlated with PCS (r=−0.29) in OB but not in CS patients. BMI was not associated with MCS in either group. Conclusion HRQL is significantly different between OB and CS patients. Surprisingly, after adjusting for symptom count, OB patients showed worse mental health scores than the CS population. Significant differences in HRQL and symptom count may suggest which OB patients should be screened for CS. PMID:23444412
Mahalakshmi, Manni Mohanraj; Bhavadharini, Balaji; Maheswari, Kumar; Anjana, Ranjit Mohan; Jebarani, Saravanan; Ninov, Lyudmil; Kayal, Arivudainambi; Malanda, Belma; Belton, Anne; Uma, Ram; Mohan, Viswanathan; Unnikrishnan, Ranjit
2016-01-01
Aim: To obtain information on existing practices in the diagnosis and management of gestational diabetes mellitus (GDM) among physicians/diabetologists/endocrinologists and obstetricians/gynecologists (OB/GYNs) in India. Methods: Details regarding diagnostic criteria used, screening methods, management strategies, and the postpartum follow-up of GDM were obtained from physicians/diabetologists/endocrinologists and OB/GYNs across 24 states of India using online/in-person surveys using a structured questionnaire. Results: A total of 3841 doctors participated in the survey of whom 68.6% worked in private clinics. Majority of OB/GYNs (84.9%) preferred universal screening for GDM, and screening in the first trimester was performed by 67% of them. Among the OB/GYNs, 600 (36.7%) reported using the nonfasting 2 h criteria for diagnosing GDM whereas 560 (29.4%) of the diabetologists/endocrinologists reported using the same. However, further questioning on the type of blood sample collected and the glucose load used revealed that, in reality, only 208 (12.7%) and 72 (3.8%), respectively, used these criteria properly. The survey also revealed that the International Association of Diabetes and Pregnancy Study Groups criteria was followed properly by 299 (18.3%) of OB/GYNs and 376 (19.7%) of physicians/diabetologists/endocrinologists. Postpartum oral glucose tolerance testing was advised by 56% of diabetologists and 71.6% of OB/GYNs. Conclusion: More than half of the physicians/diabetologists/endocrinologists and OB/GYNs in India do not follow any of the recommended guidelines for the diagnosis of GDM. This emphasizes the need for increased awareness about screening and diagnosis of GDM both among physicians/diabetologists/endocrinologists and OB/GYNs in India. PMID:27186555
Cani, P D; Possemiers, S; Van de Wiele, T; Guiot, Y; Everard, A; Rottier, O; Geurts, L; Naslain, D; Neyrinck, A; Lambert, D M; Muccioli, G G; Delzenne, N M
2009-08-01
Obese and diabetic mice display enhanced intestinal permeability and metabolic endotoxaemia that participate in the occurrence of metabolic disorders. Our recent data support the idea that a selective increase of Bifidobacterium spp. reduces the impact of high-fat diet-induced metabolic endotoxaemia and inflammatory disorders. Here, we hypothesised that prebiotic modulation of gut microbiota lowers intestinal permeability, by a mechanism involving glucagon-like peptide-2 (GLP-2) thereby improving inflammation and metabolic disorders during obesity and diabetes. Study 1: ob/ob mice (Ob-CT) were treated with either prebiotic (Ob-Pre) or non-prebiotic carbohydrates as control (Ob-Cell). Study 2: Ob-CT and Ob-Pre mice were treated with GLP-2 antagonist or saline. Study 3: Ob-CT mice were treated with a GLP-2 agonist or saline. We assessed changes in the gut microbiota, intestinal permeability, gut peptides, intestinal epithelial tight-junction proteins ZO-1 and occludin (qPCR and immunohistochemistry), hepatic and systemic inflammation. Prebiotic-treated mice exhibited a lower plasma lipopolysaccharide (LPS) and cytokines, and a decreased hepatic expression of inflammatory and oxidative stress markers. This decreased inflammatory tone was associated with a lower intestinal permeability and improved tight-junction integrity compared to controls. Prebiotic increased the endogenous intestinotrophic proglucagon-derived peptide (GLP-2) production whereas the GLP-2 antagonist abolished most of the prebiotic effects. Finally, pharmacological GLP-2 treatment decreased gut permeability, systemic and hepatic inflammatory phenotype associated with obesity to a similar extent as that observed following prebiotic-induced changes in gut microbiota. We found that a selective gut microbiota change controls and increases endogenous GLP-2 production, and consequently improves gut barrier functions by a GLP-2-dependent mechanism, contributing to the improvement of gut barrier functions during obesity and diabetes.
Meijome, Tomas E.; Baughman, Jenna T.; Hooker, R. Adam; Cheng, Ying-Hua; Ciovacco, Wendy A.; Balamohan, Sanjeev M.; Srinivasan, Trishya L.; Chitteti, Brahmananda R.; Eleniste, Pierre P.; Horowitz, Mark C.; Srour, Edward F.; Bruzzaniti, Angela; Fuchs, Robyn K.; Kacena, Melissa A.
2016-01-01
C-Mpl is the receptor for thrombopoietin (TPO), the main megakaryocyte (MK) growth factor, and c-Mpl is believed to be expressed on cells of the hematopoietic lineage. As MKs have been shown to enhance bone formation, it may be expected that mice in which c-Mpl was globally knocked out (c-Mpl−/− mice) would have decreased bone mass because they have fewer MKs. Instead, c-Mpl−/− mice have a higher bone mass than WT controls. Using c-Mpl−/− mice we investigated the basis for this discrepancy and discovered that c-Mpl is expressed on both osteoblasts (OBs) and osteoclasts (OCs), an unexpected finding that prompted us to examine further how c-Mpl regulates bone. Static and dynamic bone histomorphometry parameters suggest that c-Mpl deficiency results in a high bone turnover state with a net gain in bone volume. In vitro, a higher percentage of c-Mpl−/− OBs were in active phases of the cell cycle, leading to an increased number of OBs. No difference in OB differentiation was observed in vitro as examined by real-time PCR and functional assays. In co-culture systems, which allow for the interaction between OBs and OC progenitors, c-Mpl−/− OBs enhanced osteoclastogenesis. Two of the major signaling pathways by which OBs regulate osteoclastogenesis, MCSF/OPG/RANKL and EphrinB2-EphB2/B4, were unaffected in c-Mpl−/− OBs. These data provide new findings for the role of MKs and c-Mpl expression in bone and may provide insight into the homeostatic regulation of bone mass as well as bone loss diseases such as osteoporosis. PMID:26375403
Guimarães, Ernesto da Silveira Goulart; de Caires Júnior, Luiz Carlos; Musso, Camila Manso; Macedo de Almeida, Mariana; Gonçalves, Cássio Francisco; Pettersen, Klaus Grossi; Paes, Santiago Tavares; González Garcia, Raúl Marcel; de Freitas Mathias, Paulo Cesar; Torrezan, Rosana; Mourao-Júnior, Carlos Alberto; Andreazzi, Ana Eliza
2017-04-01
Obesity is a metabolic and hormonal disorder with serious social and psychological impacts. There is a close relationship among obesity, neuroendocrine homeostasis and behavioral patterns. However, few data are available in the literature regarding this subject. This study assessed behavior and memory of adult obese rats by monosodium l-glutamate (MSG) neonatal treatment or highly palatable dietary treatment. MSG obesity was induced by subcutaneous injections of MSG (4 mg/g) during the first 5 days of life (Ob-MSG); control group (C-MSG), received saline solution equimolar. Both groups were fed with commercial chow. To induce dietary obesity, 21-day-old rats were assigned to two experimental diets: highly palatable diet (Ob-Diet) and control diet (C-Diet) composed of commercial chow. Ninety-day-old animals were submitted to behavioral assessment by the open-field test and short- and long-term memory by the object recognition test. Biometric variables were obtained, the Lee index was calculated and mass of retroperitoneal and perigonadal fat pads was measured. Furthermore, an altered behavioral profile was investigated by quantification of plasmatic corticosterone, expression, and activity of hypothalamic extracellular signal-regulated kinase protein (ERK) 1 and 2. Increased Lee index and fat pads were observed in Ob-MSG and Ob-Diet groups. Ob-MSG presented a higher level of anxiety and impaired long-term memory compared to C-MSG, while there was no difference between Ob-Diet and C-Diet. The Ob-MSG group presented a higher level of plasmatic corticosterone and increased phosphorylation of hypothalamic ERK1 and 2. Both treatments induced obesity but only Ob-MSG showed altered behavioral parameters, which is related to increased concentration of corticosterone and hypothalamic ERK1 and 2 activation.
Xu, Jialin; Kulkarni, Supriya R; Li, Liya; Slitt, Angela L
2012-02-01
UDP-glucuronosyltransferases (Ugt) catalyze phase II conjugation reactions with glucuronic acid, which enhances chemical polarity and the elimination from the body. Few studies have addressed whether Ugt expression and activity are affected by liver disease, such as steatosis. The purpose of this study was to determine whether steatosis induced by obesity or fasting could affect liver Ugt mRNA expression and activity. Male C57BL/6J and Lep(ob/ob) (ob/ob) mice were fed ad libitum or food was withheld for 24 h. In steatotic livers of ob/ob mice, Ugt1a1, -1a6, -1a9, -2a3, -3a1, and -3a2 mRNA expression increased. Fasting, which also induced steatosis, increased hepatic Ugt1a1, -1a6, -1a7, -1a9, -2b1, -2b5, -2a3, -3a1, and -3a2 mRNA expression in mouse liver. Likewise, acetaminophen glucuronidation increased by 47% in hepatic microsomes from ob/ob mice compared with that in C57BL/6J mice, but not after fasting. In both steatosis models, Ugt induction was accompanied by increased aryl hydrocarbon receptor, constitutive androstane receptor (CAR), peroxisome proliferator-activated receptor (PPAR)-α, pregnane X receptor, nuclear factor (erythroid-derived 2)-like 2 (Nrf2), and peroxisome proliferator-activated receptor-γ coactivator-1α mRNA expression. In addition, fasting increased CAR, PPAR, and Nrf2 binding activity. The work points to hepatic triglyceride concentrations corresponding with nuclear receptor and Ugt expression. The findings indicate that steatosis significantly alters hepatic Ugt expression and activity, which could have a significant impact on determining circulating hormone levels, drug efficacy, and environmental chemical clearance.
Mendelson, M; Borowik, A; Michallet, A-S; Perrin, C; Monneret, D; Faure, P; Levy, P; Pépin, J-L; Wuyam, B; Flore, P
2016-02-01
Decreased sleep duration and altered sleep quality are risk factors for obesity in youth. Structured exercise training has been shown to increase sleep duration and improve sleep quality. This study aimed at evaluating the impact of exercise training for improving sleep duration, sleep quality and physical activity in obese adolescents (OB). Twenty OB (age: 14.5 ± 1.5 years; body mass index: 34.0 ± 4.7 kg m(-2) ) and 20 healthy-weight adolescents (HW) completed an overnight polysomnography and wore an accelerometer (SenseWear Bodymedia) for 7 days. OB participated in a 12-week supervised exercise-training programme consisting of 180 min of exercise weekly. Exercise training was a combination of aerobic exercise and resistance training. Sleep duration was greater in HW compared with OB (P < 0.05). OB presented higher apnoea-hypopnoea index than HW (P < 0.05). Physical activity (average daily metabolic equivalent of tasks [METs]) by accelerometer was lower in OB (P < 0.05). After exercise training, obese adolescents increased their sleep duration (+64.4 min; effect size: 0.88; P = 0.025) and sleep efficiency (+7.6%; effect size: 0.76; P = 0.028). Physical activity levels were increased in OB as evidenced by increased steps per day and average daily METs (P < 0.05). Improved sleep duration was associated with improved average daily METs (r = 0.48, P = 0.04). The present study confirms altered sleep duration and quality in OB. Exercise training improves sleep duration, sleep quality and physical activity. © 2015 World Obesity.
Activation of ER stress and mTORC1 suppresses hepatic sortilin-1 levels in obese mice
Ai, Ding; Baez, Juan M.; Jiang, Hongfeng; Conlon, Donna M.; Hernandez-Ono, Antonio; Frank-Kamenetsky, Maria; Milstein, Stuart; Fitzgerald, Kevin; Murphy, Andrew J.; Woo, Connie W.; Strong, Alanna; Ginsberg, Henry N.; Tabas, Ira; Rader, Daniel J.; Tall, Alan R.
2012-01-01
Recent GWAS have identified SNPs at a human chromosom1 locus associated with coronary artery disease risk and LDL cholesterol levels. The SNPs are also associated with altered expression of hepatic sortilin-1 (SORT1), which encodes a protein thought to be involved in apoB trafficking and degradation. Here, we investigated the regulation of Sort1 expression in mouse models of obesity. Sort1 expression was markedly repressed in both genetic (ob/ob) and high-fat diet models of obesity; restoration of hepatic sortilin-1 levels resulted in reduced triglyceride and apoB secretion. Mouse models of obesity also exhibit increased hepatic activity of mammalian target of rapamycin complex 1 (mTORC1) and ER stress, and we found that administration of the mTOR inhibitor rapamycin to ob/ob mice reduced ER stress and increased hepatic sortilin-1 levels. Conversely, genetically increased hepatic mTORC1 activity was associated with repressed Sort1 and increased apoB secretion. Treating WT mice with the ER stressor tunicamycin led to marked repression of hepatic sortilin-1 expression, while administration of the chemical chaperone PBA to ob/ob mice led to amelioration of ER stress, increased sortilin-1 expression, and reduced apoB and triglyceride secretion. Moreover, the ER stress target Atf3 acted at the SORT1 promoter region as a transcriptional repressor, whereas knockdown of Atf3 mRNA in ob/ob mice led to increased hepatic sortilin-1 levels and decreased apoB and triglyceride secretion. Thus, in mouse models of obesity, induction of mTORC1 and ER stress led to repression of hepatic Sort1 and increased VLDL secretion via Atf3. This pathway may contribute to dyslipidemia in metabolic disease. PMID:22466652
Donepudi, Ajay C; Cheng, Qiuqiong; Lu, Zhenqiang James; Cherrington, Nathan J; Slitt, Angela L
2016-04-01
Metabolic syndrome is a multifactorial disease associated with obesity, insulin resistance, diabetes, and the alteration of multiple metabolic hormones. Obesity rates have been rising worldwide, which increases our need to understand how this population will respond to drugs and exposure to other chemicals. The purpose of this study was to determine in lean and obese mice the ontogeny of clinical biomarkers such as serum hormone and blood glucose levels as well as the physiologic markers that correlate with nuclear receptor- and transporter-related pathways. Livers from male and female wild-type (WT) (C57BL/6) and ob/ob mice littermates were collected before, during, and after the onset of obesity. Serum hormone and mRNA levels were analyzed. Physiologic changes and gene expression during maturation and progression to obesity were performed and correlation analysis was performed using canonical correlations. Significant ontogenic changes in both WT and ob/ob mice were observed and these ontogenic changes differ in ob/ob mice with the development of obesity. In males and females, the ontogenic pattern of the expression of genes such as Abcc3, 4, Abcg2, Cyp2b10, and 4a14 started to differ from week 3, and became significant at weeks 4 and 8 in ob/ob mice compared with WT mice. In obese males, serum resistin, glucagon, and glucose levels correlated with the expression of most hepatic ATP-binding cassette (Abc) transporters, whereas in obese females, serum glucagon-like peptide 1 levels were correlated with most hepatic uptake transporters and P450 enzymes. Overall, the correlation between physiologic changes and gene expression indicate that metabolism-related hormones may play a role in regulating the genes involved in drug metabolism and transport. Copyright © 2016 by The American Society for Pharmacology and Experimental Therapeutics.
Bhetwal, Bhupal P.; An, Changlong; Baker, Salah A.; Lyon, Kristin L.
2013-01-01
Diabetic gastroparesis is a common complication of diabetes, adversely affecting quality of life with symptoms of abdominal discomfort, nausea, and vomiting. The pathogenesis of this complex disorder is not well understood, involving abnormalities in the extrinsic and enteric nervous systems, interstitial cells of Cajal (ICCs), smooth muscles and immune cells. The ob/ob mouse model of obesity and diabetes develops delayed gastric emptying, providing an animal model for investigating how gastric smooth muscle dysfunction contributes to the pathophysiology of diabetic gastroparesis. Although ROCK2, MYPT1, and CPI-17 activities are reduced in intestinal motility disorders, their functioning has not been investigated in diabetic gastroparesis. We hypothesized that reduced expression and phosphorylation of the myosin light chain phosphatase (MLCP) inhibitory proteins MYPT1 and CPI-17 in ob/ob gastric antrum smooth muscles could contribute to the impaired antrum smooth muscle function of diabetic gastroparesis. Spontaneous and carbachol- and high K+-evoked contractions of gastric antrum smooth muscles from 7 to 12 week old male ob/ob mice were reduced compared to age- and strain-matched controls. There were no differences in spontaneous and agonist-evoked intracellular Ca2+ transients and myosin light chain kinase expression. The F-actin:G-actin ratios were similar. Rho kinase 2 (ROCK2) expression was decreased at both ages. Basal and agonist-evoked MYPT1 and myosin light chain 20 phosphorylation, but not CPI-17 phosphorylation, was reduced compared to age-matched controls. These findings suggest that reduced MLCP inhibition due to decreased ROCK2 phosphorylation of MYPT1 in gastric antrum smooth muscles contributes to the antral dysmotility of diabetic gastroparesis. PMID:23576331
Not all sharks are "swimming noses": variation in olfactory bulb size in cartilaginous fishes.
Yopak, Kara E; Lisney, Thomas J; Collin, Shaun P
2015-03-01
Olfaction is a universal modality by which all animals sample chemical stimuli from their environment. In cartilaginous fishes, olfaction is critical for various survival tasks including localizing prey, avoiding predators, and chemosensory communication with conspecifics. Little is known, however, about interspecific variation in olfactory capability in these fishes, or whether the relative importance of olfaction in relation to other sensory systems varies with regard to ecological factors, such as habitat and lifestyle. In this study, we have addressed these questions by directly examining interspecific variation in the size of the olfactory bulbs (OB), the region of the brain that receives the primary sensory projections from the olfactory nerve, in 58 species of cartilaginous fishes. Relative OB size was compared among species occupying different ecological niches. Our results show that the OBs maintain a substantial level of allometric independence from the rest of the brain across cartilaginous fishes and that OB size is highly variable among species. These findings are supported by phylogenetic generalized least-squares models, which show that this variability is correlated with ecological niche, particularly habitat. The relatively largest OBs were found in pelagic-coastal/oceanic sharks, especially migratory species such as Carcharodon carcharias and Galeocerdo cuvier. Deep-sea species also possess large OBs, suggesting a greater reliance on olfaction in habitats where vision may be compromised. In contrast, the smallest OBs were found in the majority of reef-associated species, including sharks from the families Carcharhinidae and Hemiscyllidae and dasyatid batoids. These results suggest that there is great variability in the degree to which these fishes rely on olfactory cues. The OBs have been widely used as a neuroanatomical proxy for olfactory capability in vertebrates, and we speculate that differences in olfactory capabilities may be the result of functional rather than phylogenetic adaptations.
Young, Bridget E; Patinkin, Zachary W; Pyle, Laura; de la Houssaye, Becky; Davidson, Barbara S; Geraghty, Sheela; Morrow, Ardythe L; Krebs, Nancy
2017-06-01
Objective Obesity in adults is associated with inflammation and oxidative stress. Whether or not this phenotype is reflected in human milk (HM) composition, or may impact infant growth remains unknown. We investigated whether HM from overweight/obese (OW/Ob) mothers exhibited higher concentrations of inflammatory cytokines and markers of oxidative stress. We also correlated these bioactive components with infant growth patterns. Methods This was an observational cohort of 56 breastfeeding mothers and their infants [33 normal weight (NW) and 23 OW/Ob]. Infants were followed until 6 months of age and HM collected at 2-weeks and 4-months. Results Markers of oxidative stress, 8-hydroxy-deoxyguanosine (8OHdG) and 4-hydroxynonenol (HNE), decreased in HM over time (p < 0.001) and did not differ between NW and OW/Ob women. Concentrations of inflammatory cytokines, IL-6, IL-8, and TNF-α, were all inter-correlated (p < 0.001) but did not differ between NW and OW/Ob women. HM fat, protein, lactose, and total calories did not differ between NW and OW/Ob women. Infant growth patterns did not differ by group. In a model of infant weight-for-length-Z score trajectory, there was a significant interaction between both lactose and 8OHdG with maternal group: HM lactose and 8OHdG concentrations were both positively associated with increases in WLZ trajectory only among infants breastfed by OW/Ob mothers. Conclusions for Practice HM composition was relatively stable between NW and OW/Ob women. In exclusively breastfed infants, HM concentrations of lactose and 8OHdG, a marker of oxidative stress, may contribute to regulation of infant weight gain, especially among infants of OW/Ob women.
Hyperleptinemia Exacerbates High-Fat Diet-Mediated Atrial Fibrosis and Fibrillation.
Fukui, Akira; Ikebe-Ebata, Yuki; Kondo, Hidekazu; Saito, Shotaro; Aoki, Kohei; Fukunaga, Naoya; Shinohara, Tetsuji; Masaki, Takayuki; Teshima, Yasushi; Takahashi, Naohiko
2017-06-01
Obesity including metabolic syndrome is an independent risk factor of atrial fibrillation (AF). Although hyperleptinemia is usually a characteristic of obese subjects, the relationship with atrial fibrosis and AF is unknown. We tested the hypothesis that high-fat diet (HFD)-induced hyperleptinemia exacerbates atrial fibrosis and AF. Eight-week-old male C57BL/6 (WT) and leptin-deficient ob/ob (Ob) mice were treated with a normal-fat diet (NFD) or 60% HFD. After 8 weeks, transesophageal burst pacing and electrophysiological study using isolated perfused hearts were performed and left atrial (LA) tissues were collected for histological analysis, hydroxyproline assay, and reverse transcription-polymerase chain reaction. HFD treatment increased body weight in both WT and Ob mice compared with NFD (both P < 0.01). In WT-HFD mice, hyperleptinemia was observed as expected. While transesophageal burst pacing invariably induced AF (8/8, 100%) in WT-HFD mice, AF was induced less frequently (1/8, 12.5%) in Ob-HFD mice (P < 0.01). In isolated perfused hearts, the interatrial conduction time was prolonged in WT-HFD mice, but not in Ob-HFD mice (P < 0.05). Masson's trichrome staining and the hydroxyproline assay revealed interstitial LA fibrosis in WT-HFD mice, which was not observed in Ob-HFD mice (P < 0.05). Upregulation of collagen1, collagen3, α-smooth muscle actin, tumor necrosis factor-α, and monocyte chemoattractant protein-1 mRNA levels was noted in WT-HFD mice LA, but attenuated in Ob-HFD mice LA. Our findings suggest that hyperleptinemia exacerbates HFD-mediated atrial fibrosis and AF. Inhibition of leptin signaling may become a novel therapeutic target to prevent obesity-related AF. © 2017 Wiley Periodicals, Inc.
Park, Mi-Young; Jang, Hwan-Hee; Kim, Jung Bong; Yoon, Hyun Nye; Lee, Jin-Young; Lee, Young-Min; Kim, Jae-Hyun
2011-01-01
Dietary intake of whole grains reduces the incidence of chronic diseases such as obesity, diabetes, cardiovascular disease, and cancer. In an earlier study, we showed that Panicum miliaceum L. extract (PME) exhibited the highest anti-lipogenic activity in 3T3-L1 cells among extracts of nine different cereal grains tested. In this study, we hypothesized that PME in the diet would lead to weight loss and augmentation of hyperlipidemia by regulating fatty acid metabolism. PME was fed to ob/ob mice at 0%, 0.5%, or 1% (w/w) for 4 weeks. After the experimental period, body weight changes, blood serum and lipid profiles, hepatic fatty acid metabolism-related gene expression, and white adipose tissue (WAT) fatty acid composition were determined. We found that the 1% PME diet, but not the 0.5%, effectively decreased body weight, liver weight, and blood triglyceride and total cholesterol levels (P < 0.05) compared to obese ob/ob mice on a normal diet. Hepatic lipogenic-related gene (PPARα, L-FABP, FAS, and SCD1) expression decreased, whereas lipolysis-related gene (CPT1) expression increased in animals fed the 1% PME diet (P < 0.05). Long chain fatty acid content and the ratio of C18:1/C18:0 fatty acids decreased significantly in adipose tissue of animals fed the 1% PME diet (P < 0.05). Serum inflammatory mediators also decreased significantly in animals fed the 1% PME diet compared to those of the ob/ob control group (P < 0.05). These results suggest that PME is useful in the chemoprevention or treatment of obesity and obesity-related disorders. PMID:22259675
Sonne, Si Brask; Yadav, Rachita; Yin, Guangliang; Dalgaard, Marlene Danner; Myrmel, Lene Secher; Gupta, Ramneek; Wang, Jun; Madsen, Lise; Kajimura, Shingo; Kristiansen, Karsten
2017-04-03
The present study aimed to identify genes exhibiting concomitant obesity-dependent changes in DNA methylation and gene expression in adipose tissues in the mouse using diet-induced obese (DIO) C57BL/6J and genetically obese ob/ob mice as models. Mature adipocytes were isolated from epididymal and inguinal adipose tissues of ob/ob and DIO C57BL/6J mice. DNA methylation was analyzed by MeDIP-sequencing and gene expression by microarray analysis. The majority of differentially methylated regions (DMRs) were hypomethylated in obese mice. Global methylation of long interspersed elements indicated that hypomethylation did not reflect methyl donor deficiency. In both DIO and ob/ob mice, we observed more obesity-associated methylation changes in epididymal than in inguinal adipocytes. Assignment of DMRs to promoter, exon, intron and intergenic regions demonstrated that DIO-induced changes in DNA methylation in C57BL/6J mice occurred primarily in exons, whereas inguinal adipocytes of ob/ob mice exhibited a higher enrichment of DMRs in promoter regions than in other regions of the genome, suggesting an influence of leptin on DNA methylation in inguinal adipocytes. We observed altered methylation and expression of 9 genes in epididymal adipocytes, including the known obesity-associated genes, Ehd2 and Kctd15, and a novel candidate gene, Irf8, possibly involved in immune type 1/type2 balance. The use of 2 obesity models enabled us to dissociate changes associated with high fat feeding from those associated with obesity per se. This information will be of value in future studies on the mechanisms governing the development of obesity and changes in adipocyte function associated with obesity.
Huang, S-L; Yu, R-T; Gong, J; Feng, Y; Dai, Y-L; Hu, F; Hu, Y-H; Tao, Y-D; Leng, Y
2012-05-01
Arctigenin is a natural compound that had never been previously demonstrated to have a glucose-lowering effect. Here it was found to activate AMP-activated protein kinase (AMPK), and the mechanism by which this occurred, as well as the effects on glucose and lipid metabolism were investigated. 2-Deoxyglucose uptake and AMPK phosphorylation were examined in L6 myotubes and isolated skeletal muscle. Gluconeogenesis and lipid synthesis were evaluated in rat primary hepatocytes. The acute and chronic effects of arctigenin on metabolic abnormalities were observed in C57BL/6J and ob/ob mice. Changes in mitochondrial membrane potential were measured using the J-aggregate-forming dye, JC-1. Analysis of respiration of L6 myotubes or isolated mitochondria was conducted in a channel oxygen system. Arctigenin increased AMPK phosphorylation and stimulated glucose uptake in L6 myotubes and isolated skeletal muscles. In primary hepatocytes, it decreased gluconeogenesis and lipid synthesis. The enhancement of glucose uptake and suppression of hepatic gluconeogenesis and lipid synthesis by arctigenin were prevented by blockade of AMPK activation. The respiration of L6 myotubes or isolated mitochondria was inhibited by arctigenin with a specific effect on respiratory complex I. A single oral dose of arctigenin reduced gluconeogenesis in C57BL/6J mice. Chronic oral administration of arctigenin lowered blood glucose and improved lipid metabolism in ob/ob mice. This study demonstrates a new role for arctigenin as a potent indirect activator of AMPK via inhibition of respiratory complex I, with beneficial effects on metabolic disorders in ob/ob mice. This highlights the potential value of arctigenin as a possible treatment of type 2 diabetes.
Soil moisture retrieval by active/passive microwave remote sensing data
NASA Astrophysics Data System (ADS)
Wu, Shengli; Yang, Lijuan
2012-09-01
This study develops a new algorithm for estimating bare surface soil moisture using combined active / passive microwave remote sensing on the basis of TRMM (Tropical Rainfall Measuring Mission). Tropical Rainfall Measurement Mission was jointly launched by NASA and NASDA in 1997, whose main task was to observe the precipitation of the area in 40 ° N-40 ° S. It was equipped with active microwave radar sensors (PR) and passive sensor microwave imager (TMI). To accurately estimate bare surface soil moisture, precipitation radar (PR) and microwave imager (TMI) are simultaneously used for observation. According to the frequency and incident angle setting of PR and TMI, we first need to establish a database which includes a large range of surface conditions; and then we use Advanced Integral Equation Model (AIEM) to calculate the backscattering coefficient and emissivity. Meanwhile, under the accuracy of resolution, we use a simplified theoretical model (GO model) and the semi-empirical physical model (Qp Model) to redescribe the process of scattering and radiation. There are quite a lot of parameters effecting backscattering coefficient and emissivity, including soil moisture, surface root mean square height, correlation length, and the correlation function etc. Radar backscattering is strongly affected by the surface roughness, which includes the surface root mean square roughness height, surface correlation length and the correlation function we use. And emissivity is differently affected by the root mean square slope under different polarizations. In general, emissivity decreases with the root mean square slope increases in V polarization, and increases with the root mean square slope increases in H polarization. For the GO model, we found that the backscattering coefficient is only related to the root mean square slope and soil moisture when the incident angle is fixed. And for Qp Model, through the analysis, we found that there is a quite good relationship between Qpparameter and root mean square slope. So here, root mean square slope is a parameter that both models shared. Because of its big influence to backscattering and emissivity, we need to throw it out during the process of the combination of GO model and Qp model. The result we obtain from the combined model is the Fresnel reflection coefficient in the normal direction gama(0). It has a good relationship with the soil dielectric constant. In Dobson Model, there is a detailed description about Fresnel reflection coefficient and soil moisture. With the help of Dobson model and gama(0) that we have obtained, we can get the soil moisture that we want. The backscattering coefficient and emissivity data used in combined model is from TRMM/PR, TMI; with this data, we can obtain gama(0); further, we get the soil moisture by the relationship of the two parameters-- gama(0) and soil moisture. To validate the accuracy of the retrieval soil moisture, there is an experiment conducted in Tibet. The soil moisture data which is used to validate the retrieval algorithm is from GAME-Tibet IOP98 Soil Moisture and Temperature Measuring System (SMTMS). There are 9 observing sites in SMTMS to validate soil moisture. Meanwhile, we use the SMTMS soil moisture data obtained by Time Domain Reflectometer (TDR) to do the validation. And the result shows the comparison of retrieval and measured results is very good. Through the analysis, we can see that the retrieval and measured results in D66 is nearly close; and in MS3608, the measured result is a little higher than retrieval result; in MS3637, the retrieval result is a little higher than measured result. According to the analysis of the simulation results, we found that this combined active and passive approach to retrieve the soil moisture improves the retrieval accuracy.