Separated flow over bodies of revolution using an unsteady discrete-vorticity cross wake. Part 2: Computer program description

NASA Technical Reports Server (NTRS)

Marshall, F. J.; Deffenbaugh, F. D.

1974-01-01

A method is developed to determine the flow field of a body of revolution in separated flow. The computer was used to integrate various solutions and solution properties of the sub-flow fields which made up the entire flow field without resorting to a finite difference solution to the complete Navier-Stokes equations. The technique entails the use of the unsteady cross flow analogy and a new solution to the two-dimensional unsteady separated flow problem based upon an unsteady, discrete-vorticity wake. Data for the forces and moments on aerodynamic bodies at low speeds and high angle of attack (outside the range of linear inviscid theories) such that the flow is substantially separated are produced which compare well with experimental data. In addition, three dimensional steady separated regions and wake vortex patterns are determined. The computer program developed to perform the numerical calculations is described.

A Theory of Material Spike Formation in Flow Separation

NASA Astrophysics Data System (ADS)

Serra, Mattia; Haller, George

2017-11-01

We develop a frame-invariant theory of material spike formation during flow separation over a no-slip boundary in two-dimensional flows with arbitrary time dependence. This theory identifies both fixed and moving separation, is effective also over short-time intervals, and admits a rigorous instantaneous limit. Our theory is based on topological properties of material lines, combining objectively stretching- and rotation-based kinematic quantities. The separation profile identified here serves as the theoretical backbone for the material spike from its birth to its fully developed shape, and remains hidden to existing approaches. Finally, our theory can be used to rigorously explain the perception of off-wall separation in unsteady flows, and more importantly, provide the conditions under which such a perception is justified. We illustrate our results in several examples including steady, time-periodic and unsteady analytic velocity fields with flat and curved boundaries, and an experimental dataset.

Model of separation performance of bilinear gradients in scanning format counter-flow gradient electrofocusing techniques.

PubMed

Shameli, Seyed Mostafa; Glawdel, Tomasz; Ren, Carolyn L

2015-03-01

Counter-flow gradient electrofocusing allows the simultaneous concentration and separation of analytes by generating a gradient in the total velocity of each analyte that is the sum of its electrophoretic velocity and the bulk counter-flow velocity. In the scanning format, the bulk counter-flow velocity is varying with time so that a number of analytes with large differences in electrophoretic mobility can be sequentially focused and passed by a single detection point. Studies have shown that nonlinear (such as a bilinear) velocity gradients along the separation channel can improve both peak capacity and separation resolution simultaneously, which cannot be realized by using a single linear gradient. Developing an effective separation system based on the scanning counter-flow nonlinear gradient electrofocusing technique usually requires extensive experimental and numerical efforts, which can be reduced significantly with the help of analytical models for design optimization and guiding experimental studies. Therefore, this study focuses on developing an analytical model to evaluate the separation performance of scanning counter-flow bilinear gradient electrofocusing methods. In particular, this model allows a bilinear gradient and a scanning rate to be optimized for the desired separation performance. The results based on this model indicate that any bilinear gradient provides a higher separation resolution (up to 100%) compared to the linear case. This model is validated by numerical studies. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Base flow of streams in the outcrop area of southeastern sand aquifer, South Carolina, Georgia, Alabama, and Mississippi

USGS Publications Warehouse

Stricker, Virginia

1983-01-01

The base flow component of streamflow was separated from hydrographs for unregulated streams in the Cretaceous and Tertiary clastic outcrop area of South Carolina, Georgia, Alabama, and Mississippi. The base flow values are used in estimating recharge to the sand aquifer. Relations developed between mean annual base flow and stream discharge at the 60- and 65-percent streamflow duration point can be used to approximate mean annual base flow in lieu of hydrograph separation methods for base flows above 10 cu ft/s. Base flow recession curves were used to derive estimates of hydraulic diffusivity of the aquifer which was converted to transmissivity using estimated specific yield. These base-flow-derived transmissivities are in general agreement with transmissivities derived from well data. The shape of flow duration curves of streams is affected by the lithology of the Coastal Plain sediments. Steep flow duration curves appear to be associated with basins underlain by clay or chalk where a low percentage of the discharge is base flow while flatter curves appear to be associated with basins underlain by sand and gravel where a high percentage of the discharge is base flow. (USGS)

Process for separating carbon dioxide from flue gas using sweep-based membrane separation and absorption steps

DOEpatents

Wijmans, Johannes G.; Baker, Richard W.; Merkel, Timothy C.

2012-08-21

A gas separation process for treating flue gases from combustion processes, and combustion processes including such gas separation. The invention involves routing a first portion of the flue gas stream to be treated to an absorption-based carbon dioxide capture step, while simultaneously flowing a second portion of the flue gas across the feed side of a membrane, flowing a sweep gas stream, usually air, across the permeate side, then passing the permeate/sweep gas to the combustor.

Novel surface modification of polymer-based separation media controlling separation selectivity, retentivity and generation of electroosmotic flow.

PubMed

Hosoya, Ken; Kubo, Takuya; Takahashi, Katsuo; Ikegami, Tohru; Tanaka, Nobuo

2002-12-06

Uniformly sized packing materials based on synthetic polymer particles for high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC) have been prepared from polymerization mixtures containing methacrylic acid (MAA) as a functional monomer and by using a novel surface modification method. This "dispersion method" affords effectively modified separation media. Both the amount of MAA utilized in the preparation and reaction time affect the selectivity of chromatographic separation in both the HPLC and the CEC mode and electroosmotic flow. This detailed study revealed that the dispersion method effectively modified internal surface of macroporous separation media and, based on the amount of MAA introduced, exclusion mechanism for the separation of certain solutes could be observed.

Systems for column-based separations, methods of forming packed columns, and methods of purifying sample components

DOEpatents

Egorov, Oleg B.; O'Hara, Matthew J.; Grate, Jay W.; Chandler, Darrell P.; Brockman, Fred J.; Bruckner-Lea, Cynthia J.

2000-01-01

The invention encompasses systems for column-based separations, methods of packing and unpacking columns and methods of separating components of samples. In one aspect, the invention includes a method of packing and unpacking a column chamber, comprising: a) packing a matrix material within a column chamber to form a packed column; and b) after the packing, unpacking the matrix material from the column chamber without moving the column chamber. In another aspect, the invention includes a system for column-based separations, comprising: a) a fluid passageway, the fluid passageway comprising a column chamber and a flow path in fluid communication with the column chamber, the flow path being obstructed by a retaining material permeable to a carrier fluid and impermeable to a column matrix material suspended in the carrier fluid, the flow path extending through the column chamber and through the retaining material, the flow path being configured to form a packed column within the column chamber when a suspension of the fluid and the column matrix material is flowed along the flow path; and b) the fluid passageway extending through a valve intermediate the column chamber and the retaining material.

Systems For Column-Based Separations, Methods Of Forming Packed Columns, And Methods Of Purifying Sample Components

DOEpatents

Egorov, Oleg B.; O'Hara, Matthew J.; Grate, Jay W.; Chandler, Darrell P.; Brockman, Fred J.; Bruckner-Lea, Cynthia J.

2006-02-21

The invention encompasses systems for column-based separations, methods of packing and unpacking columns and methods of separating components of samples. In one aspect, the invention includes a method of packing and unpacking a column chamber, comprising: a) packing a matrix material within a column chamber to form a packed column; and b) after the packing, unpacking the matrix material from the column chamber without moving the column chamber. In another aspect, the invention includes a system for column-based separations, comprising: a) a fluid passageway, the fluid passageway comprising a column chamber and a flow path in fluid communication with the column chamber, the flow path being obstructed by a retaining material permeable to a carrier fluid and impermeable to a column matrix material suspended in the carrier fluid, the flow path extending through the column chamber and through the retaining material, the flow path being configured to form a packed column within the column chamber when a suspension of the fluid and the column matrix material is flowed along the flow path; and b) the fluid passageway extending through a valve intermediate the column chamber and the retaining material.

Systems For Column-Based Separations, Methods Of Forming Packed Columns, And Methods Of Purifying Sample Components.

DOEpatents

Egorov, Oleg B.; O'Hara, Matthew J.; Grate, Jay W.; Chandler, Darrell P.; Brockman, Fred J.; Bruckner-Lea, Cynthia J.

2004-08-24

The invention encompasses systems for column-based separations, methods of packing and unpacking columns and methods of separating components of samples. In one aspect, the invention includes a method of packing and unpacking a column chamber, comprising: a) packing a matrix material within a column chamber to form a packed column; and b) after the packing, unpacking the matrix material from the column chamber without moving the column chamber. In another aspect, the invention includes a system for column-based separations, comprising: a) a fluid passageway, the fluid passageway comprising a column chamber and a flow path in fluid communication with the column chamber, the flow path being obstructed by a retaining material permeable to a carrier fluid and impermeable to a column matrix material suspended in the carrier fluid, the flow path extending through the column chamber and through the retaining material, the flow path being configured to form a packed column within the column chamber when a suspension of the fluid and the column matrix material is flowed along the flow path; and b) the fluid passageway extending through a valve intermediate the column chamber and the retaining material.

A Novel Approach to Adaptive Flow Separation Control

DTIC Science & Technology

2016-09-03

particular, it considers control of flow separation over a NACA-0025 airfoil using microjet actuators and develops Adaptive Sampling Based Model...Predictive Control ( Adaptive SBMPC), a novel approach to Nonlinear Model Predictive Control that applies the Minimal Resource Allocation Network...Distribution Unlimited UU UU UU UU 03-09-2016 1-May-2013 30-Apr-2016 Final Report: A Novel Approach to Adaptive Flow Separation Control The views, opinions

Sheathless focusing and separation of microparticles using tilted angle travelling surface acoustic waves.

PubMed

Ahmed, Husnain; Destgeer, Ghulam; Park, Jinsoo; Afzal, Muhammad; Sung, Hyung Jin

2018-06-18

The sheathless focusing and separation of microparticles is an important pre-processing step in various biochemical assays in which enriched sample isolation is critical. Most previous microfluidic particle separation techniques have used a sheath flow to achieve efficient sample focusing. The sheath flow diluted the analyte, and required additional microchannels and accurate flow control. We demonstrated a tilted angle travelling surface acoustic wave (taTSAW)-based sheathless focusing and separation of particles in a continuous flow. The proposed device consisted of a piezoelectric substrate with a pair of interdigitated transducers (IDTs) deposited at two different angles relative to the flow direction. A Y-shaped polydimethylsiloxane (PDMS) microchannel having one inlet and two outlet ports was positioned on top of the IDTs such that the acoustic energy coupling into the fluid was maximized and wave attenuation by the PDMS walls was minimized. The two IDTs independently produced high-frequency taTSAWs, which propagated at ±30° with respect to the flow direction and imparted a direct acoustic radiation force onto the target particles. A sample mixture containing 4.8 and 3.2 µm particles was focused and then separated by the actuation of the IDTs at 194 and 136 MHz frequencies, respectively, without using an additional sheath flow. The proposed taTSAW-based particle separation device offered a high purity > 99% at the both outlets over a wide range of flow speeds (up to 83.3 mm/s).

Stability of spanwise-modulated flows behind backward-facing steps

NASA Astrophysics Data System (ADS)

Boiko, A. V.; Dovgal, A. V.; Sorokin, A. M.

2017-10-01

An overview and synthesis of researches on development of local vortical disturbances in laminar separated flows downstream of backward-facing steps, in which the velocity field depends essentially on two variables are given. Peculiarities of transition to turbulence in such spatially inhomogeneous separated zones are discussed. The experimental data are supplemented by the linear stability characteristics of model velocity profiles of the separated flow computed using both the classical local formulation and the nonlocal approach based on the Floquet theory for partial differential equations with periodic coefficients. The results clarify the response of the local separated flows to their modulation with stationary geometrical and temperature inhomogeneities. The results can be useful for the development of new methods of laminar separation control.

Effects of Wall-Normal and Angular Momentum Injections in Airfoil Separation Control

NASA Astrophysics Data System (ADS)

Munday, Phillip M.; Taira, Kunihiko

2018-05-01

The objective of this computational study is to quantify the influence of wall-normal and angular momentum injections in suppressing laminar flow separation over a canonical airfoil. Open-loop control of fully separated, incompressible flow over a NACA 0012 airfoil at $\\alpha = 9^\\circ$ and $Re = 23,000$ is examined with large-eddy simulations. This study independently introduces wall-normal momentum and angular momentum into the separated flow using swirling jets through model boundary conditions. The response of the flow field and the surface vorticity fluxes to various combinations of actuation inputs are examined in detail. It is observed that the addition of angular momentum input to wall-normal momentum injection enhances the suppression of flow separation. Lift enhancement and suppression of separation with the wall-normal and angular momentum inputs are characterized by modifying the standard definition of the coefficient of momentum. The effect of angular momentum is incorporated into the modified coefficient of momentum by introducing a characteristic swirling jet velocity based on the non-dimensional swirl number. With this single modified coefficient of momentum, we are able to categorize each controlled flow into separated, transitional, and attached flows.

On recent developments in marginal separation theory.

PubMed

Braun, S; Scheichl, S

2014-07-28

Thin aerofoils are prone to localized flow separation at their leading edge if subjected to moderate angles of attack α. Although 'laminar separation bubbles' at first do not significantly alter the aerofoil performance, they tend to 'burst' if α is increased further or if perturbations acting upon the flow reach a certain intensity. This then either leads to global flow separation (stall) or triggers the laminar-turbulent transition process within the boundary layer flow. This paper addresses the asymptotic analysis of the early stages of the latter phenomenon in the limit as the characteristic Reynolds number [Formula: see text], commonly referred to as marginal separation theory. A new approach based on the adjoint operator method is presented that enables the fundamental similarity laws of marginal separation theory to be derived and the analysis to be extended to higher order. Special emphasis is placed on the breakdown of the flow description, i.e. the formation of finite-time singularities (a manifestation of the bursting process), and on its resolution being based on asymptotic arguments. The passage to the subsequent triple-deck stage is described in detail, which is a prerequisite for carrying out a future numerical treatment of this stage in a proper way. Moreover, a composite asymptotic model is developed in order for the inherent ill-posedness of the Cauchy problems associated with the current flow description to be resolved.

Evolution of shock-induced pressure on a flat-face/flat-base body and afterbody flow separation

NASA Technical Reports Server (NTRS)

Yoshikawa, K. K.; Wray, A. A.

1982-01-01

The time-dependent, compressible Reynolds-averaged, Navier-Stokes equations are applied to solve an axisymmetric supersonic flow around a flat-face/flat-base body with and without a sting support. Important transient phenomena, not yet well understood, are investigated, and the significance of the present solution to the phenomena is discussed. The phenomena, described in detail, are as follows: the transient formation of the bow and recompression shock waves; the evolution of a pressure buildup due to diffraction of the incident shock wave in the forebody and afterbody regions, including the luminosity accompanying the pressure buildup; the separation of the flow as influenced by pressure buildup; the location of the separation and the reattachment points; and the transient period of the shock-induced base flow. The important influence of the nonsteady (transient) and steady flow on the aerodynamic characteristics, radiative heat transfer, and, thus, on the survivability or safeguard problems for an aircraft fuselage, missile, or planetary entry probe at very high flight speeds is described.

Flow-separation patterns on symmetric forebodies

NASA Technical Reports Server (NTRS)

Keener, Earl R.

1986-01-01

Flow-visualization studies of ogival, parabolic, and conical forebodies were made in a comprehensive investigation of the various types of flow patterns. Schlieren, vapor-screen, oil-flow, and sublimation flow-visualization tests were conducted over an angle-of-attack range from 0 deg. to 88 deg., over a Reynolds-number range from 0.3X10(6) to 2.0X10(6) (based on base diameter), and over a Mach number range from 0.1 to 2. The principal effects of angle of attack, Reynolds number, and Mach number on the occurrence of vortices, the position of vortex shedding, the principal surface-flow-separation patterns, the magnitude of surface-flow angles, and the extent of laminar and turbulent flow for symmetric, asymmetric, and wake-like flow-separation regimes are presented. It was found that the two-dimensional cylinder analogy was helpful in a qualitative sense in analyzing both the surface-flow patterns and the external flow field. The oil-flow studies showed three types of primary separation patterns at the higher Reynolds numbers owing to the influence of boundary-layer transition. The effect of angle of attack and Reynolds number is to change the axial location of the onset and extent of the primary transitional and turbulent separation regions. Crossflow inflectional-instability vortices were observed on the windward surface at angles of attack from 5 deg. to 55 deg. Their effect is to promote early transition. At low angles of attack, near 10 deg., an unexpected laminar-separation bubble occurs over the forward half of the forebody. At high angles of attack, at which vortex asymmetry occurs, the results support the proposition that the principal cause of vortex asymmetry is the hydrodynamic instability of the inviscid flow field. On the other hand, boundary-layer asymmetries also occur, especially at transitional Reynolds numbers. The position of asymmetric vortex shedding moves forward with increasing angle of attack and with increasing Reynolds number, and moves rearward with increasing Mach number.

Dynamics of Active Separation Control at High Reynolds Numbers

NASA Technical Reports Server (NTRS)

Pack, LaTunia G.; Seifert, Avi

2000-01-01

A series of active flow control experiments were recently conducted at high Reynolds numbers on a generic separated configuration. The model simulates the upper surface of a 20% thick Glauert-Goldschmied type airfoil at zero angle of attack. The flow is fully turbulent since the tunnel sidewall boundary layer flows over the model. The main motivation for the experiments is to generate a comprehensive data base for validation of unsteady numerical simulation as a first step in the development of a CFD design tool, without which it would not be possible to effectively utilize the great potential of unsteady flow control. This paper focuses on the dynamics of several key features of the baseline as well as the controlled flow. It was found that the thickness of the upstream boundary layer has a negligible effect on the flow dynamics. It is speculated that separation is caused mainly by the highly convex surface while viscous effects are less important. The two-dimensional separated flow contains unsteady waves centered on a reduced frequency of 0.9, while in the three dimensional separated flow, frequencies around a reduced frequency of 0.3 and 1 are active. Several scenarios of resonant wave interaction take place at the separated shear-layer and in the pressure recovery region. The unstable reduced frequency bands for periodic excitation are centered on 1.5 and 5, but these reduced frequencies are based on the length of the baseline bubble that shortens due to the excitation. The conventional works well for the coherent wave features. Reproduction of these dynamic effects by a numerical simulation would provide benchmark validation.

Using resolvent analysis for the design of separation control on a NACA 0012 airfoil

NASA Astrophysics Data System (ADS)

Yeh, Chi-An; Taira, Kunihiko

2017-11-01

A combined effort based on large-eddy simulation and resolvent analysis on the separated flow over a NACA 0012 airfoil is conducted to design active flow control for suppression of separation. This study considers the the airfoil at 6 deg. angle-of-attack and Reynolds number of 23000. The response mode obtained from the resolvent analysis about the baseline turbulent mean flow reveals modal structures that can be categorized into three families when sweeping through the resonant frequency: (1) von Karman wake structure for low frequency; (2) Kelvin-Helmholtz structure in the separation bubble for high frequency; (3) blended structure of (1) and (2) for the intermediate frequency. Leveraging the insights from resolvent analysis, unsteady thermal actuation is introduced to the flow near the leading-edge to examine the use of the frequencies from three families for separation control in LES. As indicated by the resolvent response modes, we find that the use of intermediate frequencies are most effective in suppressing the flow separation, since the shear layer over the separation bubble and the wake are both receptive to the perturbation at the these frequencies. The resolvent-analysis-based control strategy achieves 35% drag reduction and 9% lift increase with effective frequency. This work was supported by Office of Naval Research (N00014-15-R-FO13) and Army Research Office (W911NF-14-1-0224).

Towards DMD-Based Estimation and Control of Flow Separation using an Array of Surface Pressure Sensors

NASA Astrophysics Data System (ADS)

Deem, Eric; Cattafesta, Louis; Zhang, Hao; Rowley, Clancy

2016-11-01

Closed-loop control of flow separation requires the spatio-temporal states of the flow to be fed back through the controller in real time. Previously, static and dynamic estimation methods have been employed that provide reduced-order model estimates of the POD-coefficients of the flow velocity using surface pressure measurements. However, this requires a "learning" dataset a priori. This approach is effective as long as the dynamics during control do not stray from the learning dataset. Since only a few dynamical features are required for feedback control of flow separation, many of the details provided by full-field snapshots are superfluous. This motivates a state-observation technique that extracts key dynamical features directly from surface pressure, without requiring PIV snapshots. The results of identifying DMD modes of separated flow through an array of surface pressure sensors in real-time are presented. This is accomplished by employing streaming DMD "on the fly" to surface pressure snapshots. These modal characteristics exhibit striking similarities to those extracted from PIV data and the pressure field obtained via solving Poisson's equation. Progress towards closed-loop separation control based on the dynamic modes of surface pressure will be discussed. Supported by AFOSR Grant FA9550-14-1-0289.

Low speed streak formation in a separating turbulent boundary layer

NASA Astrophysics Data System (ADS)

Santos, Leonardo; Lang, Amy; Wahidi, Redha; Bonacci, Andrew

2017-11-01

Separation control mechanisms present on the skin of the shortfin mako shark may permit higher swimming speeds. The morphology of the scales varies over the entire body, with maximum scale flexibility found on the flank region with an adverse pressure gradient(APG). It is hypothesized that reversing flow close the skin bristles the scales inhibiting further flow reversal and controlling flow separation. Experiments are conducted in water tunnel facility and the flow field of a separating turbulent boundary layer(TBL) is measured using DPIV and Insight V3V. Flow separation is induced by a rotating cylinder which generates a controlled APG over a flat plate (Re = 510000 and 620000). Specifically, the low speed streak(LSS) formation is documented and matches predicted sizing based on viscous length scale calculations. It is surmised that shark scale width corresponds to this LSS sizing for real swimming TBL conditions. However, flow separation control has been demonstrated over real skin specimens under much lower speed conditions which indicates the mechanism is fairly Re independent if multiple scales are bristled as the width of the LSS increases. The formation of reversing flow within the streaks is studied specifically to better understand the process by which this flow initiates scale bristling on shortfin mako skin as a passive, flow actuated separation control mechanism. The authors would like to greatefully acknowledge the Army Research Office for funding this project.

VORSTAB: A computer program for calculating lateral-directional stability derivatives with vortex flow effect

NASA Technical Reports Server (NTRS)

Lan, C. Edward

1985-01-01

A computer program based on the Quasi-Vortex-Lattice Method of Lan is presented for calculating longitudinal and lateral-directional aerodynamic characteristics of nonplanar wing-body combination. The method is based on the assumption of inviscid subsonic flow. Both attached and vortex-separated flows are treated. For the vortex-separated flow, the calculation is based on the method of suction analogy. The effect of vortex breakdown is accounted for by an empirical method. A summary of the theoretical method, program capabilities, input format, output variables and program job control set-up are described. Three test cases are presented as guides for potential users of the code.

Numerical techniques for the solution of the compressible Navier-Stokes equations and implementation of turbulence models. [separated turbulent boundary layer flow problems

NASA Technical Reports Server (NTRS)

Baldwin, B. S.; Maccormack, R. W.; Deiwert, G. S.

1975-01-01

The time-splitting explicit numerical method of MacCormack is applied to separated turbulent boundary layer flow problems. Modifications of this basic method are developed to counter difficulties associated with complicated geometry and severe numerical resolution requirements of turbulence model equations. The accuracy of solutions is investigated by comparison with exact solutions for several simple cases. Procedures are developed for modifying the basic method to improve the accuracy. Numerical solutions of high-Reynolds-number separated flows over an airfoil and shock-separated flows over a flat plate are obtained. A simple mixing length model of turbulence is used for the transonic flow past an airfoil. A nonorthogonal mesh of arbitrary configuration facilitates the description of the flow field. For the simpler geometry associated with the flat plate, a rectangular mesh is used, and solutions are obtained based on a two-equation differential model of turbulence.

Large-eddy simulation of flow in a plane, asymmetric diffuser

NASA Technical Reports Server (NTRS)

Kaltenbach, Hans-Jakob

1993-01-01

Recent improvements in subgrid-scale modeling as well as increases in computer power make it feasible to investigate flows using large-eddy simulation (LES) which have been traditionally studied with techniques based on Reynolds averaging. However, LES has not yet been applied to many flows of immediate technical interest. Preliminary results from LES of a plane diffuser flow are described. The long term goal of this work is to investigate flow separation as well as separation control in ducts and ramp-like geometries.

Stochastic Estimation and Non-Linear Wall-Pressure Sources in a Separating/Reattaching Flow

NASA Technical Reports Server (NTRS)

Naguib, A.; Hudy, L.; Humphreys, W. M., Jr.

2002-01-01

Simultaneous wall-pressure and PIV measurements are used to study the conditional flow field associated with surface-pressure generation in a separating/reattaching flow established over a fence-with-splitter-plate geometry. The conditional flow field is captured using linear and quadratic stochastic estimation based on the occurrence of positive and negative pressure events in the vicinity of the mean reattachment location. The results shed light on the dominant flow structures associated with significant wall-pressure generation. Furthermore, analysis based on the individual terms in the stochastic estimation expansion shows that both the linear and non-linear flow sources of the coherent (conditional) velocity field are equally important contributors to the generation of the conditional surface pressure.

Juncture flow improvement for wing/pylon configurations by using CFD methodology

NASA Technical Reports Server (NTRS)

Gea, Lie-Mine; Chyu, Wei J.; Stortz, Michael W.; Chow, Chuen-Yen

1993-01-01

Transonic flow field around a fighter wing/pylon configuration was simulated by using an implicit upwinding Navier-Stokes flow solver (F3D) and overset grid technology (Chimera). Flow separation and local shocks near the wing/pylon junction were observed in flight and predicted by numerical calculations. A new pylon/fairing shape was proposed to improve the flow quality. Based on numerical results, the size of separation area is significantly reduced and the onset of separation is delayed farther downstream. A smoother pressure gradient is also obtained near the junction area. This paper demonstrates that computational fluid dynamics (CFD) methodology can be used as a practical tool for aircraft design.

A direct-inverse method for transonic and separated flows about airfoils

NASA Technical Reports Server (NTRS)

Carlson, Leland A.

1990-01-01

A direct-inverse technique and computer program called TAMSEP that can be used for the analysis of the flow about airfoils at subsonic and low transonic freestream velocities is presented. The method is based upon a direct-inverse nonconservative full potential inviscid method, a Thwaites laminar boundary layer technique, and the Barnwell turbulent momentum integral scheme; and it is formulated using Cartesian coordinates. Since the method utilizes inverse boundary conditions in regions of separated flow, it is suitable for predicting the flow field about airfoils having trailing edge separated flow under high lift conditions. Comparisons with experimental data indicate that the method should be a useful tool for applied aerodynamic analyses.

Dynamics of Active Separation Control at High Reynolds Numbers

NASA Technical Reports Server (NTRS)

Pack, LaTunia G.; Seifert, Avi

2000-01-01

A series of active flow control experiments were recently conducted at high Reynolds numbers on a generic separated configuration. The model simulates the upper surface of a 20% thick Glauert-Goldschmied type airfoil at zero angle of attack. The flow is fully turbulent since the tunnel sidewall boundary layer flows over the model. The main motivation for the experiments is to generate a comprehensive data base for validation of unsteady numerical simulation as a first step in the development of a CFD design tool, without which it would not be possible to effectively utilize the great potential of unsteady flow control. This paper focuses on the dynamics of several key features of the baseline as well as the controlled flow. It was found that the thickness of the upstream boundary layer has a negligible effect on the flow dynamics. It is speculated that separation is caused mainly by the highly convex surface while viscous effects are less important. The two-dimensional separated flow contains unsteady waves centered on a reduced frequency of 0.8, while in the three dimensional separated flow, frequencies around a reduced frequency of 0.3 and 1 are active. Several scenarios of resonant wave interaction take place at the separated shear-layer and in the pressure recovery region. The unstable reduced frequency bands for periodic excitation are centered on 1.5 and 5, but these reduced frequencies are based on the length of the baseline bubble that shortens due to the excitation. The conventional swept wing-scaling works well for the coherent wave features. Reproduction of these dynamic effects by a numerical simulation would provide benchmark validation.

A Preliminary Assessment of Phase Separator Ground-Based and Reduced-Gravity Testing for ALS Systems

NASA Technical Reports Server (NTRS)

Hall, Nancy Rabel

2006-01-01

A viewgraph presentation of phase separator ground-based and reduced-gravity testing for Advanced Life Support (ALS) systems is shown. The topics include: 1) Multiphase Flow Technology Program; 2) Types of Separators; 3) MOBI Phase Separators; 4) Experiment set-up; and 5) Preliminary comparison/results.

Active flow separation control by a position-based iterative learning control algorithm with experimental validation

NASA Astrophysics Data System (ADS)

Cai, Zhonglun; Chen, Peng; Angland, David; Zhang, Xin

2014-03-01

A novel iterative learning control (ILC) algorithm was developed and applied to an active flow control problem. The technique uses pulsed air jets to delay flow separation on a two-element high-lift wing. The ILC algorithm uses position-based pressure measurements to update the actuation. The method was experimentally tested on a wing model in a 0.9 m × 0.6 m low-speed wind tunnel at the University of Southampton. Compressed air and fast switching solenoid valves were used as actuators to excite the flow, and the pressure distribution around the chord of the wing was measured as a feedback control signal for the ILC controller. Experimental results showed that the actuation was able to delay the separation and increase the lift by approximately 10%-15%. By using the ILC algorithm, the controller was able to find the optimum control input and maintain the improvement despite sudden changes of the separation position.

Paramagnetic capture mode magnetophoretic microseparator for high efficiency blood cell separations.

PubMed

Han, Ki-Ho; Frazier, A Bruno

2006-02-01

This paper presents the characterization of continuous single-stage and three-stage cascade paramagnetic capture (PMC) mode magnetophoretic microseparators for high efficiency separation of red and white blood cells from diluted whole blood based on their native magnetic properties. The separation mechanism for both PMC microseparators is based on a high gradient magnetic separation (HGMS) method. This approach enables separation of blood cells without the use of additives such as magnetic beads. Experimental results for the single-stage PMC microseparator show that 91.1% of red blood cells were continuously separated from the sample at a volumetric flow rate of 5 microl h-1. In addition, the three-stage cascade PMC microseparator continuously separated 93.5% of red blood cells and 97.4% of white blood cells from whole blood at a volumetric flow rate of 5 microl h-1.

Cavity-induced microstreaming for simultaneous on-chip pumping and size-based separation of cells and particles.

PubMed

Patel, Maulik V; Nanayakkara, Imaly A; Simon, Melinda G; Lee, Abraham P

2014-10-07

We present a microfluidic platform for simultaneous on-chip pumping and size-based separation of cells and particles without external fluidic control systems required for most existing platforms. The device utilizes an array of acoustically actuated air/liquid interfaces generated using dead-end side channels termed Lateral Cavity Acoustic Transducers (LCATs). The oscillating interfaces generate local streaming flow while the angle of the LCATs relative to the main channel generates a global bulk flow from the inlet to the outlet. The interaction of these two competing velocity fields (i.e. global bulk velocity vs. local streaming velocity) is responsible for the observed separation. It is shown that the separation of 5 μm and 10 μm polystyrene beads is dependent on the ratio of these two competing velocity fields. The experimental and simulation results suggest that particle trajectories based only on Stokes drag force cannot fully explain the separation behavior and that the impact of additional forces due to the oscillating flow field must be considered to determine the trajectory of the beads and ultimately the separation behavior of the device. To demonstrate an application of this separation platform with cellular components, smaller red blood cells (7.5 ± 0.8 μm) are separated from larger K562 cells (16.3 ± 2.0 μm) with viabilities comparable to those of controls based on a trypan blue exclusion assay.

HYSEP: A Computer Program for Streamflow Hydrograph Separation and Analysis

USGS Publications Warehouse

Sloto, Ronald A.; Crouse, Michele Y.

1996-01-01

HYSEP is a computer program that can be used to separate a streamflow hydrograph into base-flow and surface-runoff components. The base-flow component has traditionally been associated with ground-water discharge and the surface-runoff component with precipitation that enters the stream as overland runoff. HYSEP includes three methods of hydrograph separation that are referred to in the literature as the fixed interval, sliding-interval, and local-minimum methods. The program also describes the frequency and duration of measured streamflow and computed base flow and surface runoff. Daily mean stream discharge is used as input to the program in either an American Standard Code for Information Interchange (ASCII) or binary format. Output from the program includes table,s graphs, and data files. Graphical output may be plotted on the computer screen or output to a printer, plotter, or metafile.

Disturbance Source Separation in Shear Flows Using Blind Source Separation Methods

NASA Astrophysics Data System (ADS)

Gluzman, Igal; Cohen, Jacob; Oshman, Yaakov

2017-11-01

A novel approach is presented for identifying disturbance sources in wall-bounded shear flows. The method can prove useful for active control of boundary layer transition from laminar to turbulent flow. The underlying idea is to consider the flow state, as measured in sensors, to be a mixture of sources, and to use Blind Source Separation (BSS) techniques to recover the separate sources and their unknown mixing process. We present a BSS method based on the Degenerate Unmixing Estimation Technique. This method can be used to identify any (a priori unknown) number of sources by using the data acquired by only two sensors. The power of the new method is demonstrated via numerical and experimental proofs of concept. Wind tunnel experiments involving boundary layer flow over a flat plate were carried out, in which two hot-wire anemometers were used to separate disturbances generated by disturbance generators such as a single dielectric barrier discharge plasma actuator and a loudspeaker.

Controlling mixing and segregation in time periodic granular flows

NASA Astrophysics Data System (ADS)

Bhattacharya, Tathagata

Segregation is a major problem for many solids processing industries. Differences in particle size or density can lead to flow-induced segregation. In the present work, we employ the discrete element method (DEM)---one type of particle dynamics (PD) technique---to investigate the mixing and segregation of granular material in some prototypical solid handling devices, such as a rotating drum and chute. In DEM, one calculates the trajectories of individual particles based on Newton's laws of motion by employing suitable contact force models and a collision detection algorithm. Recently, it has been suggested that segregation in particle mixers can be thwarted if the particle flow is inverted at a rate above a critical forcing frequency. Further, it has been hypothesized that, for a rotating drum, the effectiveness of this technique can be linked to the probability distribution of the number of times a particle passes through the flowing layer per rotation of the drum. In the first portion of this work, various configurations of solid mixers are numerically and experimentally studied to investigate the conditions for improved mixing in light of these hypotheses. Besides rotating drums, many studies of granular flow have focused on gravity driven chute flows owing to its practical importance in granular transportation and to the fact that the relative simplicity of this type of flow allows for development and testing of new theories. In this part of the work, we observe the deposition behavior of both mono-sized and polydisperse dry granular materials in an inclined chute flow. The effects of different parameters such as chute angle, particle size, falling height and charge amount on the mass fraction distribution of granular materials after deposition are investigated. The simulation results obtained using DEM are compared with the experimental findings and a high degree of agreement is observed. Tuning of the underlying contact force parameters allows the achievement of realistic results and is used as a means of validating the model against available experimental data. The tuned model is then used to find the critical chute length for segregation based on the hypothesis that segregation can be thwarted if the particle flow is inverted at a rate above a critical forcing frequency. The critical frequency, fcrit, is inversely proportional to the characteristic time of segregation, ts. Mixing is observed instead of segregation when the chute length L < U avgts, where Uavg denotes the average stream-wise flow velocity of the particles. While segregation is often an undesired effect, sometimes separating the components of a particle mixture is the ultimate goal. Rate-based separation processes hold promise as both more environmentally benign as well as less energy intensive when compared to conventional particle separations technologies such as vibrating screens or flotation methods. This approach is based on differences in the kinetic properties of the components of a mixture, such as the velocity of migration or diffusivity. In this portion of the work, two examples of novel rate-based separation devices are demonstrated. The first example involves the study of the dynamics of gravity-driven particles through an array of obstacles. Both discrete element (DEM) simulations and experiments are used to augment the understanding of this device. Dissipative collisions (both between the particles themselves and with the obstacles) give rise to a diffusive motion of particles perpendicular to the flow direction and the differences in diffusion lengths are exploited to separate the particles. The second example employs DEM to analyze a ratchet mechanism where a current of particles can be produced in a direction perpendicular to the energy input. In this setup, a vibrating saw-toothed base is employed to induce different mobility for different types of particles. The effect of operating conditions and design parameters on the separation efficiency are discussed. Keywords: granular flow, particle, mixing, segregation, discrete element method, particle dynamics, tumbler, chute, periodic flow inversion, collisional flow, rate-based separation, ratchet, static separator, dissipative particle dynamics, non-spherical droplet.

Afterbody Drag. Volume 3. Literature Survey.

DTIC Science & Technology

1980-06-01

Investigacion Aeronautica y Espacial , Cordoba, Argentine, Rep. 01-65, June 1965. de Krasinski, J., "A Study of Separated Base Flow Behind Bodies of...Investigacion Aeronautica y Espacial , Cordoba, Argentine, January 1966. de Krasinski, J., "Study of Separated Base Flow Behind Bodies of Revolution in...Investigacion Aeronautica y Espacial , Cordoba, Argentine, June 1966. De Kuyper, R.E., "Investigation of Plume Effects on the Stability Charac- teristics of a

Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP).

PubMed

Moon, Hui-Sung; Kwon, Kiho; Kim, Seung-Il; Han, Hyunju; Sohn, Joohyuk; Lee, Soohyeon; Jung, Hyo-Il

2011-03-21

Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer, so their isolations and quantifications are important for biomedical applications such as cancer prognosis and measuring the responses to drug treatments. In this paper, we present the development of a microfluidic device for the separation of CTCs from blood cells based on the physical properties of cells. For use as a CTC model, we successfully separated human breast cancer cells (MCF-7) from a spiked blood cell sample by combining multi-orifice flow fractionation (MOFF) and dielectrophoretic (DEP) cell separation technique. Hydrodynamic separation takes advantage of the massive and high-throughput filtration of blood cells as it can accommodate a very high flow rate. DEP separation plays a role in precise post-processing to enhance the efficiency of the separation. The serial combination of these two different sorting techniques enabled high-speed continuous flow-through separation without labeling. We observed up to a 162-fold increase in MCF-7 cells at a 126 µL min(-1) flow rate. Red and white blood cells were efficiently removed with separation efficiencies of 99.24% and 94.23% respectively. Therefore, we suggest that our system could be used for separation and detection of CTCs from blood cells for biomedical applications. This journal is © The Royal Society of Chemistry 2011

Quantitative identification of riverine nitrogen from point, direct runoff and base flow sources.

PubMed

Huang, Hong; Zhang, Baifa; Lu, Jun

2014-01-01

We present a methodological example for quantifying the contributions of riverine total nitrogen (TN) from point, direct runoff and base flow sources by combining a recursive digital filter technique and statistical methods. First, we separated daily riverine flow into direct runoff and base flow using a recursive digital filter technique; then, a statistical model was established using daily simultaneous data for TN load, direct runoff rate, base flow rate, and temperature; and finally, the TN loading from direct runoff and base flow sources could be inversely estimated. As a case study, this approach was adopted to identify the TN source contributions in Changle River, eastern China. Results showed that, during 2005-2009, the total annual TN input to the river was 1,700.4±250.2 ton, and the contributions of point, direct runoff and base flow sources were 17.8±2.8%, 45.0±3.6%, and 37.2±3.9%, respectively. The innovation of the approach is that the nitrogen from direct runoff and base flow sources could be separately quantified. The approach is simple but detailed enough to take the major factors into account, providing an effective and reliable method for riverine nitrogen loading estimation and source apportionment.

Continuous separation of multiple size microparticles using alternating current dielectrophoresis in microfluidic device with acupuncture needle electrodes

NASA Astrophysics Data System (ADS)

Tao, Ye; Ren, Yukun; Yan, Hui; Jiang, Hongyuan

2016-03-01

The need to continuously separate multiple microparticles is required for the recent development of lab-on-chip technology. Dielectrophoresis(DEP)-based separation device is extensively used in kinds of microfluidic applications. However, such conventional DEP-based device is relatively complicated and difficult for fabrication. A concise microfluidic device is presented for effective continuous separation of multiple size particle mixtures. A pair of acupuncture needle electrodes are creatively employed and embedded in a PDMS(poly-dimethylsiloxane) hurdle for generating non-uniform electric field thereby achieving a continuous DEP separation. The separation mechanism is that the incoming particle samples with different sizes experience different negative DEP(nDEP) forces and then they can be transported into different downstream outlets. The DEP characterizations of particles are calculated, and their trajectories are numerically predicted by considering the combined action of the incoming laminar flow and the nDEP force field for guiding the separation experiments. The device performance is verified by successfully separating a three-sized particle mixture, including polystyrene microspheres with diameters of 3 μm, 10 μm and 25 μm. The separation purity is below 70% when the flow rate ratio is less than 3.5 or more than 5.1, while the separation purity can be up to more than 90% when the flow rate ratio is between 3.5 and 5.1 and meanwhile ensure the voltage output falls in between 120 V and 150 V. Such simple DEP-based separation device has extensive applications in future microfluidic systems.

Protein separation through preliminary experiments concerning pH and salt concentration by tube radial distribution chromatography based on phase separation multiphase flow using a polytetrafluoroethylene capillary tube.

PubMed

Kan, Hyo; Tsukagoshi, Kazuhiko

2017-07-01

Protein mixtures were separated using tube radial distribution chromatography (TRDC) in a polytetrafluoroethylene (PTFE) capillary (internal diameter=100µm) separation tube. Separation by TRDC is based on the annular flow in phase separation multiphase flow and features an open-tube capillary without the use of specific packing agents or application of high voltages. Preliminary experiments were conducted to examine the effects of pH and salt concentration on the phase diagram of the ternary mixed solvent solution of water-acetonitrile-ethyl acetate (8:2:1 volume ratio) and on the TRDC system using the ternary mixed solvent solution. A model protein mixture containing peroxidase, lysozyme, and bovine serum albumin was analyzed via TRDC with the ternary mixed solvent solution at various pH values, i.e., buffer-acetonitrile-ethyl acetate (8:2:1 volume ratio). Protein was separated on the chromatograms by the TRDC system, where the elution order was determined by the relation between the isoelectric points of protein and the pH values of the solvent solution. Copyright © 2017 Elsevier B.V. All rights reserved.

Towards an entropy-based detached-eddy simulation

NASA Astrophysics Data System (ADS)

Zhao, Rui; Yan, Chao; Li, XinLiang; Kong, WeiXuan

2013-10-01

A concept of entropy increment ratio ( s¯) is introduced for compressible turbulence simulation through a series of direct numerical simulations (DNS). s¯ represents the dissipation rate per unit mechanical energy with the benefit of independence of freestream Mach numbers. Based on this feature, we construct the shielding function f s to describe the boundary layer region and propose an entropy-based detached-eddy simulation method (SDES). This approach follows the spirit of delayed detached-eddy simulation (DDES) proposed by Spalart et al. in 2005, but it exhibits much better behavior after their performances are compared in the following flows, namely, pure attached flow with thick boundary layer (a supersonic flat-plate flow with high Reynolds number), fully separated flow (the supersonic base flow), and separated-reattached flow (the supersonic cavity-ramp flow). The Reynolds-averaged Navier-Stokes (RANS) resolved region is reliably preserved and the modeled stress depletion (MSD) phenomenon which is inherent in DES and DDES is partly alleviated. Moreover, this new hybrid strategy is simple and general, making it applicable to other models related to the boundary layer predictions.

A direct-inverse method for transonic and separated flows about airfoils

NASA Technical Reports Server (NTRS)

Carlson, K. D.

1985-01-01

A direct-inverse technique and computer program called TAMSEP that can be sued for the analysis of the flow about airfoils at subsonic and low transonic freestream velocities is presented. The method is based upon a direct-inverse nonconservative full potential inviscid method, a Thwaites laminar boundary layer technique, and the Barnwell turbulent momentum integral scheme; and it is formulated using Cartesian coordinates. Since the method utilizes inverse boundary conditions in regions of separated flow, it is suitable for predicing the flowfield about airfoils having trailing edge separated flow under high lift conditions. Comparisons with experimental data indicate that the method should be a useful tool for applied aerodynamic analyses.

Experimental Investigation Of Base Flow Buffeting On The Ariane 5 Launcher Using High Speed PIV

NASA Astrophysics Data System (ADS)

Schrijer, F. F. J.; Sciacchitano, A.; Scarrano, F.; Hannemann, K.; Pallegoix, J.-F.; Maseand, J. E. J.; Schwane, R.

2011-05-01

Experiments have been performed on a 1:60 scale Ariane 5 launcher in the DNW HST wind tunnel by means of two-component particle image velocimetry (2C-PIV). Measurements are performed for Mach 0.5 and Mach 0.8. The investigation focuses on studying the flow-buffeting phenomenon in the base of an Ariane V rocket. In total four configurations are tested: reference configuration, skirt, scoop and reference configuration without struts. It has been found that the presence of the struts has a large effect on the flow field; the secondary flow caused by the struts decreases the separated region and increases the overall turbulence. The skirt has the effect that the shear layer separates later and therefore does not reattach on the nozzle. Also in the separated region a secondary recirculation region is formed. For the scoop configuration it was observed that a second wake was formed by the scoop element causing the overall shear layer to become thicker. Finally using POD analysis two dominant modes are identified that can be associated to the separation bubble and shear layer dynamics.

Oxy-fired boiler unit and method of operating the same

DOEpatents

Lou, Xinsheng; Zhang, Jundong; Joshi, Abhinaya; McCombe, James A.; Levasseur, Armand A.

2016-12-06

An oxy-combustion boiler unit is disclosed which includes a furnace for combusting fuel and for emitting flue gas resulting from combustion. The furnace has first, second and third combustion zones, and an air separation unit for separating oxygen gas from air and providing a first portion of the separated oxygen to a first oxidant flow, a second portion to a second oxidant flow, and a third portion of the separated oxygen gas to the first, second, and third zones of the furnace. A controller can cause the separated oxygen gas to be distributed so that the first and second oxygen flows have a desired oxygen content, and so that the first, second, and third zones of the furnace receive a desired amount of oxygen based on a combustion zone stoichiometry control.

Comparison of liquid rocket engine base region heat flux computations using three turbulence models

NASA Technical Reports Server (NTRS)

Kumar, Ganesh N.; Griffith, Dwaine O., II; Prendergast, Maurice J.; Seaford, C. M.

1993-01-01

The flow in the base region of launch vehicles is characterized by flow separation, flow reversals, and reattachment. Computation of the convective heat flux in the base region and on the nozzle external surface of Space Shuttle Main Engine and Space Transportation Main Engine (STME) is an important part of defining base region thermal environments. Several turbulence models were incorporated in a CFD code and validated for flow and heat transfer computations in the separated and reattaching regions associated with subsonic and supersonic flows over backward facing steps. Heat flux computations in the base region of a single STME engine and a single S1C engine were performed using three different wall functions as well as a renormalization-group based k-epsilon model. With the very limited data available, the computed values are seen to be of the right order of magnitude. Based on the validation comparisons, it is concluded that all the turbulence models studied have predicted the reattachment location and the velocity profiles at various axial stations downstream of the step very well.

Continuous high throughput molecular adhesion based cell sorting using ridged microchannels

NASA Astrophysics Data System (ADS)

Tasadduq, Bushra; Wang, Gonghao; Alexeev, Alexander; Sarioglu, Ali Fatih; Sulchek, Todd

2016-11-01

Cell molecular interactions govern important physiological processes such as stem cell homing, inflammation and cancer metastasis. But due to a lack of effective separation technologies selective to these interactions it is challenging to specifically sort cells. Other label free separation techniques based on size, stiffness and shape do not provide enough specificity to cell type, and correlation to clinical condition. We propose a novel microfluidic device capable of high throughput molecule dependent separation of cells by flowing them through a microchannel decorated with molecule specific coated ridges. The unique aspect of this sorting design is the use of optimized gap size which is small enough to lightly squeeze the cells while flowing under the ridged part of the channel to increase the surface area for interaction between the ligand on cell surface and coated receptor molecule but large enough so that biomechanical markers, stiffness and viscoelasticity, do not dominate the cell separation mechanism. We are able to separate Jurkat cells based on its expression of PSGL-1ligand using ridged channel coated with P selectin at a flow rate of 0.045ml/min and achieve 2-fold and 5-fold enrichment of PSGL-1 positive and negative Jurkat cells respectively.

A multiple-time-scale turbulence model based on variable partitioning of turbulent kinetic energy spectrum

NASA Technical Reports Server (NTRS)

Kim, S.-W.; Chen, C.-P.

1988-01-01

The paper presents a multiple-time-scale turbulence model of a single point closure and a simplified split-spectrum method. Consideration is given to a class of turbulent boundary layer flows and of separated and/or swirling elliptic turbulent flows. For the separated and/or swirling turbulent flows, the present turbulence model yielded significantly improved computational results over those obtained with the standard k-epsilon turbulence model.

Three-dimensional printed magnetophoretic system for the continuous flow separation of avian influenza H5N1 viruses.

PubMed

Wang, Yuhe; Li, Yanbin; Wang, Ronghui; Wang, Maohua; Lin, Jianhan

2017-04-01

As a result of the low concentration of avian influenza viruses in samples for routine screening, the separation and concentration of these viruses are vital for their sensitive detection. We present a novel three-dimensional printed magnetophoretic system for the continuous flow separation of the viruses using aptamer-modified magnetic nanoparticles, a magnetophoretic chip, a magnetic field, and a fluidic controller. The magnetic field was designed based on finite element magnetic simulation and developed using neodymium magnets with a maximum intensity of 0.65 T and a gradient of 32 T/m for dragging the nanoparticle-virus complexes. The magnetophoretic chip was designed by SOLIDWORKS and fabricated by a three-dimensional printer with a magnetophoretic channel for the continuous flow separation of the viruses using phosphate-buffered saline as carrier flow. The fluidic controller was developed using a microcontroller and peristaltic pumps to inject the carrier flow and the viruses. The trajectory of the virus-nanoparticle complexes was simulated using COMSOL for optimization of the carrier flow and the magnetic field, respectively. The results showed that the H5N1 viruses could be captured, separated, and concentrated using the proposed magnetophoretic system with the separation efficiency up to 88% in a continuous flow separation time of 2 min for a sample volume of 200 μL. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

An experimental study of separated flow on a finite wing

NASA Technical Reports Server (NTRS)

Winkelmann, A. E.

1981-01-01

The flow field associated with the formation of a mushroom shaped trailing edge stall cell on a low-aspect-ratio (AR = 4.0) wing was investigated in a series of low speed wind tunnel tests (Reynolds number based on 15.2 cm chord = 480,000). Flow field surveys of the separation bubble and wake of a partially stalled and fully stalled wing were completed using a hot-wire probe, a split-film probe, and a directional sensitive pressure probe. A new color video display technique was developed to display the flow field survey data. Photographs were obtained of surface oil flow patterns and smoke flow visualization

Passive chip-based droplet sorting

DOEpatents

Beer, Neil Reginald; Lee, Abraham P; Hatch, Andrew C; Fisher, Jeffrey S

2015-03-03

An apparatus for passive sorting of microdroplets including a main flow channel, a flow stream of microdroplets in the main flow channel wherein the microdroplets have substantially the same diameter and wherein the flow stream of microdroplets includes first microdroplets having a first degree of stiffness and second microdroplets having a second degree of stiffness wherein the second degree of stiffness is different than the first degree of stiffness. A second flow channel is connected to the main flow channel for the second microdroplets having a second degree of stiffness. A separator separates the second microdroplets having a second degree of stiffness from the first microdroplets and directs the second microdroplets having a second degree of stiffness into the second flow channel.

Numerical Simulations of Plasma Based Flow Control Applications

NASA Technical Reports Server (NTRS)

Suzen, Y. B.; Huang, P. G.; Jacob, J. D.; Ashpis, D. E.

2005-01-01

A mathematical model was developed to simulate flow control applications using plasma actuators. The effects of the plasma actuators on the external flow are incorporated into Navier Stokes computations as a body force vector. In order to compute this body force vector, the model solves two additional equations: one for the electric field due to the applied AC voltage at the electrodes and the other for the charge density representing the ionized air. The model is calibrated against an experiment having plasma-driven flow in a quiescent environment and is then applied to simulate a low pressure turbine flow with large flow separation. The effects of the plasma actuator on control of flow separation are demonstrated numerically.

An assessment of the tracer-based approach to quantifying groundwater contributions to streamflow

NASA Astrophysics Data System (ADS)

Jones, J. P.; Sudicky, E. A.; Brookfield, A. E.; Park, Y.-J.

2006-02-01

The use of conservative geochemical and isotopic tracers along with mass balance equations to determine the pre-event groundwater contributions to streamflow during a rainfall event is widely used for hydrograph separation; however, aspects related to the influence of surface and subsurface mixing processes on the estimates of the pre-event contribution remain poorly understood. Moreover, the lack of a precise definition of "pre-event" versus "event" contributions on the one hand and "old" versus "new" water components on the other hand has seemingly led to confusion within the hydrologic community about the role of Darcian-based groundwater flow during a storm event. In this work, a fully integrated surface and subsurface flow and solute transport model is used to analyze flow system dynamics during a storm event, concomitantly with advective-dispersive tracer transport, and to investigate the role of hydrodynamic mixing processes on the estimates of the pre-event component. A number of numerical experiments are presented, including an analysis of a controlled rainfall-runoff experiment, that compare the computed Darcian-based groundwater fluxes contributing to streamflow during a rainfall event with estimates of these contributions based on a tracer-based separation. It is shown that hydrodynamic mixing processes can dramatically influence estimates of the pre-event water contribution estimated by a tracer-based separation. Specifically, it is demonstrated that the actual amount of bulk flowing groundwater contributing to streamflow may be much smaller than the quantity indirectly estimated from a separation based on tracer mass balances, even if the mixing processes are weak.

Active-Adaptive Control of Inlet Separation Using Supersonic Microjets

NASA Technical Reports Server (NTRS)

Alvi, Farrukh S.

2007-01-01

Flow separation in internal and external flows generally results in a significant degradation in aircraft performance. For internal flows, such as inlets and transmission ducts in aircraft propulsion systems, separation is undesirable as it reduces the overall system performance. The aim of this research has been to understand the nature of separation and more importantly, to explore techniques to actively control it. In this research, we extended our investigation of active separation control (under a previous NASA grant) where we explored the use of microjets for the control of boundary layer separation. The geometry used for the initial study was a simple diverging Stratford ramp, equipped with arrays of microjets. These early results clearly show that the activation of microjets eliminated flow separation. Furthermore, the velocity-field measurements, using PIV, also demonstrate that the gain in momentum due to the elimination of separation is at least an order of magnitude larger (two orders of magnitude larger in most cases) than the momentum injected by the microjets and is accomplished with very little mass flow through the microjets. Based on our initial promising results this research was continued under the present grant, using a more flexible model. This model allows for the magnitude and extent of separation as well as the microjet parameters to be independently varied. The results, using this model were even more encouraging and demonstrated that microjet control completely eliminated significant regions of flow separation over a wide range of conditions with almost negligible mass flow. Detailed studies of the flowfield and its response to microjets were further examined using 3-component PIV and unsteady pressure measurements, among others. As the results presented this report will show, microjets were successfully used to control the separation of a much larger extent and magnitude than demonstrated in our earlier experiments. In fact, using the appropriate combination of control parameters (microjet, location, angle and pressure) separation was completely eliminated for the largest separated flowfield we could generate with the present model. Separation control also resulted in a significant reduction in the unsteady pressures in the flow where the unsteady pressure field was found to be directly responsive to the state of the flow above the surface. Hence, our study indicates that the unsteady pressure signature is a strong candidate for a flow state sensor , which can be used to estimate the location, magnitude and other properties of the separated flowfield. Once better understood and properly utilized, this behavior can be of significant practical importance for developing and implementing online control.

Topology of three-dimensional separated flows

NASA Technical Reports Server (NTRS)

Tobak, M.; Peake, D. J.

1981-01-01

Based on the hypothesis that patterns of skin-friction lines and external streamlines reflect the properties of continuous vector fields, topology rules define a small number of singular points (nodes, saddle points, and foci) that characterize the patterns on the surface and on particular projections of the flow (e.g., the crossflow plane). The restricted number of singular points and the rules that they obey are considered as an organizing principle whose finite number of elements can be combined in various ways to connect together the properties common to all steady three dimensional viscous flows. Introduction of a distinction between local and global properties of the flow resolves an ambiguity in the proper definition of a three dimensional separated flow. Adoption of the notions of topological structure, structural stability, and bifurcation provides a framework to describe how three dimensional separated flows originate and succeed each other as the relevant parameters of the problem are varied.

Estimates of ground-water recharge, base flow, and stream reach gains and losses in the Willamette River basin, Oregon

USGS Publications Warehouse

Lee, Karl K.; Risley, John C.

2002-03-19

Precipitation-runoff models, base-flow-separation techniques, and stream gain-loss measurements were used to study recharge and ground-water surface-water interaction as part of a study of the ground-water resources of the Willamette River Basin. The study was a cooperative effort between the U.S. Geological Survey and the State of Oregon Water Resources Department. Precipitation-runoff models were used to estimate the water budget of 216 subbasins in the Willamette River Basin. The models were also used to compute long-term average recharge and base flow. Recharge and base-flow estimates will be used as input to a regional ground-water flow model, within the same study. Recharge and base-flow estimates were made using daily streamflow records. Recharge estimates were made at 16 streamflow-gaging-station locations and were compared to recharge estimates from the precipitation-runoff models. Base-flow separation methods were used to identify the base-flow component of streamflow at 52 currently operated and discontinued streamflow-gaging-station locations. Stream gain-loss measurements were made on the Middle Fork Willamette, Willamette, South Yamhill, Pudding, and South Santiam Rivers, and were used to identify and quantify gaining and losing stream reaches both spatially and temporally. These measurements provide further understanding of ground-water/surface-water interactions.

Subsurface Supergranular Vertical Flows as Measured Using Large Distance Separations in Time-Distance Helioseismology

NASA Technical Reports Server (NTRS)

Duvall, T. L., Jr.; Hanasoge, S. M.

2012-01-01

As large-distance rays (say, 10-24 deg) approach the solar surface approximately vertically, travel times measured from surface pairs for these large separations are mostly sensitive to vertical flows, at least for shallow flows within a few Mm of the solar surface. All previous analyses of supergranulation have used smaller separations and have been hampered by the difficulty of separating the horizontal and vertical flow components. We find that the large separation travel times associated with upergranulation cannot be studied using the standard phase-speed filters of time-distance helioseismology. These filters, whose use is based upon a refractive model of the perturbations,reduce the resultant travel time signal by at least an order of magnitude at some distances. More effective filters are derived. Modeling suggests that the center-annulus travel time difference in the separation range 10-24 deg is insensitive to the horizontally diverging flow from the centers of the supergranules and should lead to a constant signal from the vertical flow. Our measurement of this quantity for the average supergranule, 5.1 s, is constant over the distance range. This magnitude of signal cannot be caused by the level of upflow at cell centers seen at the photosphere of 10 m/s extended in depth. It requires the vertical flow to increase with depth. A simple Gaussian model of the increase with depth implies a peak upward flow of 240 m/s at a depth of 2.3 Mm and a peak horizontal flow of 700 m/s at a depth of 1.6 Mm.

Continuous inertial microparticle and blood cell separation in straight channels with local microstructures.

PubMed

Wu, Zhenlong; Chen, Yu; Wang, Moran; Chung, Aram J

2016-02-07

Fluid inertia which has conventionally been neglected in microfluidics has been gaining much attention for particle and cell manipulation because inertia-based methods inherently provide simple, passive, precise and high-throughput characteristics. Particularly, the inertial approach has been applied to blood separation for various biomedical research studies mainly using spiral microchannels. For higher throughput, parallelization is essential; however, it is difficult to realize using spiral channels because of their large two dimensional layouts. In this work, we present a novel inertial platform for continuous sheathless particle and blood cell separation in straight microchannels containing microstructures. Microstructures within straight channels exert secondary flows to manipulate particle positions similar to Dean flow in curved channels but with higher controllability. Through a balance between inertial lift force and microstructure-induced secondary flow, we deterministically position microspheres and cells based on their sizes to be separated downstream. Using our inertial platform, we successfully sorted microparticles and fractionized blood cells with high separation efficiencies, high purities and high throughputs. The inertial separation platform developed here can be operated to process diluted blood with a throughput of 10.8 mL min(-1)via radially arrayed single channels with one inlet and two rings of outlets.

Calculation of symmetric and asymmetric vortex seperation on cones and tangent ogives based on discrete vortex models

NASA Technical Reports Server (NTRS)

Chin, S.; Lan, C. Edward

1988-01-01

An inviscid discrete vortex model, with newly derived expressions for the tangential velocity imposed at the separation points, is used to investigate the symmetric and asymmetric vortex separation on cones and tangent ogives. The circumferential locations of separation are taken from experimental data. Based on a slender body theory, the resulting simultaneous nonlinear algebraic equations in a cross-flow plane are solved with Broyden's modified Newton-Raphson method. Total force coefficients are obtained through momentum principle with new expressions for nonconical flow. It is shown through the method of function deflation that multiple solutions exist at large enough angles of attack, even with symmetric separation points. These additional solutions are asymmetric in vortex separation and produce side force coefficients which agree well with data for cones and tangent ogives.

The structure of dilute combusting sprays

NASA Technical Reports Server (NTRS)

Shuen, J. S.; Solomon, A. S. P.; Faeth, F. M.

1985-01-01

An experimental and theoretical study of drop processes in a turbulent flame is described. The experiments involved a monodisperse (105 and 180 micro m initial diameter) stream of methanol drops injected at the base of a turbulent methane-fueled diffusion flame burning in still air. The following measurements were made: mean and fluctuating phase velocities, mean drop number flux, drop-size distributions and mean gas-phase temperatures. Measurements were compared with predictions of two separated flow models: (1) deterministic separated flow, where drop-turbulence interactions are ignored; and (2) stochastic separated flow, where drop-turbulence interactions are considered using random-walk computations. The stochastic separated flow analysis yielded best agreement with measurements, since it provides for turbulent dispersion of drops which was important for present test conditions (and probably for most combusting sprays as well). Distinguishing the presence or absence of envelope flames around the drops, however, was relatively unimportant for present test conditions, since the drops spent most of their lifetime in fuel-rich regions of the flow where this distinction is irrelevant.

The effect of small temperature gradients on flow in a continuous flow electrophoresis chamber

NASA Technical Reports Server (NTRS)

Rhodes, P. H.; Snyder, R. S.

1982-01-01

Continuous flow electrophoresis employs an electric field to separate biological cells suspended in a flowing liquid buffer solution. Good separations based on differences in electrophoretic mobility are obtained only when a unidirectional flow is maintained. The desired flow has a parabolic structure in the narrow dimension of the chamber and is uniform acros the width, except near the edges where the no-slip condition prevails. However, because of buoyancy, very small laterall or axial temperature gradients deform the flow significantly. The results of experiments conducted with a specially instrumented chamber show the origin and structure of the buoyancy-driven perturbations. It is found that very small temperature gradients can disturb the flow significantly, as was predicted by earlier theoretical work.

Low-frequency dynamics of pressure-induced turbulent separation bubbles

NASA Astrophysics Data System (ADS)

Weiss, Julien; Mohammed-Taifour, Abdelouahab; Lefloch, Arnaud

2017-11-01

We experimentally investigate a pressure-induced turbulent separation bubble (TSB), which is generated on a flat test surface through a combination of adverse and favorable pressure gradients imposed on a nominally two-dimensional, incompressible, turbulent boundary layer. We probe the flow using piezo-resistive pressure transducers, MEMS shear-stress sensors, and high-speed, 2D-2C, PIV measurements. Through the use of Fourier analysis of the wall-pressure fluctuations and Proper Orthogonal Decomposition of the velocity fields, we show that this type of flow is characterized by a self-induced, low-frequency contraction and expansion - called breathing - of the TSB. The dominant Strouhal number of this motion, based on the TSB length and the incoming velocity in the potential flow, is of the order of 0.01. We compare this motion to the low-frequency dynamics observed in laminar separation bubbles (LSBs), geometry-induced TSBs, and shock-induced separated flows.

A point vortex model for the formation of ocean eddies by flow separation

NASA Astrophysics Data System (ADS)

Southwick, O. R.; Johnson, E. R.; McDonald, N. R.

2015-01-01

A simple model for the formation of ocean eddies by flow separation from sharply curved horizontal boundary topography is developed. This is based on the Brown-Michael model for two-dimensional vortex shedding, which is adapted to more realistically model mesoscale oceanic flow by including a deforming free surface. With a free surface, the streamfunction for the flow is not harmonic so the conformal mapping methods used in the standard Brown-Michael approach cannot be used and the problem must be solved numerically. A numerical scheme is developed based on a Chebyshev spectral method for the streamfunction partial differential equation and a second order implicit timestepping scheme for the vortex position ordinary differntial equations. This method is used to compute shed vortex trajectories for three background flows: (A) a steady flow around a semi-infinite plate, (B) a free vortex moving around a semi-infinite plate, and (C) a free vortex moving around a right-angled wedge. In (A), the inclusion of surface deformation dramatically slows the vortex and changes its trajectory from a straight path to a curved one. In (B) and (C), without the inclusion of flow separation, free vortices traverse fully around the tip along symmetrical trajectories. With the effects of flow separation included, very different trajectories are found: for all values of the model parameter—the Rossby radius—the free and shed vortices pair up and move off to infinity without passing around the tip. Their final propagation angle depends strongly and monotonically on the Rossby radius.

On-the-Fly Cross Flow Laser Guided Separation of Aerosol Particles Based on Size, Refractive Index and Density-Theoretical Analysis

DTIC Science & Technology

2010-12-20

Optical chromatography Size determination by eluting particles ,” Talanta 48(3), 551–557 (1999). 15. A. Ashkin, and J. M. Dziedzic, “Optical levitation ...the use of optical force in the gas phase, for example, levitation of airborne particles [15,16], and more recent studies on aerosol optical guiding...On-the-fly cross flow laser guided separation of aerosol particles based on size, refractive index and density–theoretical analysis A. A. Lall

Asymptotic research of transonic gas flows

NASA Astrophysics Data System (ADS)

Velmisov, Petr A.; Tamarova, Yuliya A.

2017-12-01

The article is dedicated to the development asymptotic theory of gas flowing at speed next to sound velocity, particularly of gas transonic flows, i.e. the flows, containing both, subsonic and supersonic areas. The main issue, when styding such flows, are nonlinearity and combined type of equations, describing the transonic flow. Based on asymptotic nonlinear equation obtained in the article, the gas transonic flows is studied, considering transverse disturbance with respect to the main flow. The asymptotic conditions at shock-wave front and conditions on the streamlined surface are found. Moreover, the equation of sound surface and asymptotic formula defining the pressure are recorded. Several exact particular solutions of such equation are given, and their application to solve several tasks of transonic aerodynamics is indicated. Specifically, the polynomial form solution describing gas axisymmetric flows in Laval nozzles with constant acceleration in direction of the nozzle's axis and flow swirling is obtained. The solutions describing the unsteady flow along the channels between spinning surfaces are presented. The asymptotic equation is obtained, describing the flow, appearing during non-separated and separated flow past, closely approximated to cylindrical one. Specific solutions are given, based on which the examples of steady flow are formed.

Flow Control on Low-Pressure Turbine Airfoils Using Vortex Generator Jets

NASA Technical Reports Server (NTRS)

Volino, Ralph J.; Ibrahim, Mounir B.; Kartuzova, Olga

2010-01-01

Motivation - Higher loading on Low-Pressure Turbine (LPT) airfoils: Reduce airfoil count, weight, cost. Increase efficiency, and Limited by suction side separation. Growing understanding of transition, separation, wake effects: Improved models. Take advantage of wakes. Higher lift airfoils in use. Further loading increases may require flow control: Passive: trips, dimples, etc. Active: plasma actuators, vortex generator jets (VGJs). Can increased loading offset higher losses on high lift airfoils. Objectives: Advance knowledge of boundary layer separation and transition under LPT conditions. Demonstrate, improve understanding of separation control with pulsed VGJs. Produce detailed experimental data base. Test and develop computational models.

Wavelet analysis of hemispheroid flow separation toward understanding human vocal fold pathologies

NASA Astrophysics Data System (ADS)

Plesniak, Daniel H.; Carr, Ian A.; Bulusu, Kartik V.; Plesniak, Michael W.

2014-11-01

Physiological flows observed in human vocal fold pathologies, such as polyps and nodules, can be modeled by flow over a wall-mounted protuberance. The experimental investigation of flow separation over a surface-mounted hemispheroid was performed using particle image velocimetry (PIV) and measurements of surface pressure in a low-speed wind tunnel. This study builds on the hypothesis that the signatures of vortical structures associated with flow separation are imprinted on the surface pressure distributions. Wavelet decomposition methods in one- and two-dimensions were utilized to elucidate the flow behavior. First, a complex Gaussian wavelet was used for the reconstruction of surface pressure time series from static pressure measurements acquired from ports upstream, downstream, and on the surface of the hemispheroid. This was followed by the application of a novel continuous wavelet transform algorithm (PIVlet 1.2) using a 2D-Ricker wavelet for coherent structure detection on instantaneous PIV-data. The goal of this study is to correlate phase shifts in surface pressure with Strouhal numbers associated with the vortex shedding. Ultimately, the wavelet-based analytical framework will be aimed at addressing pulsatile flows. This material is based in part upon work supported by the National Science Foundation under Grant Number CBET-1236351, and GW Center for Biomimetics and Bioinspired Engineering (COBRE).

Experimental, Theoretical, and Computational Investigation of Separated Nozzle Flows

NASA Technical Reports Server (NTRS)

Hunter, Craig A.

2004-01-01

A detailed experimental, theoretical, and computational study of separated nozzle flows has been conducted. Experimental testing was performed at the NASA Langley 16-Foot Transonic Tunnel Complex. As part of a comprehensive static performance investigation, force, moment, and pressure measurements were made and schlieren flow visualization was obtained for a sub-scale, non-axisymmetric, two-dimensional, convergent- divergent nozzle. In addition, two-dimensional numerical simulations were run using the computational fluid dynamics code PAB3D with two-equation turbulence closure and algebraic Reynolds stress modeling. For reference, experimental and computational results were compared with theoretical predictions based on one-dimensional gas dynamics and an approximate integral momentum boundary layer method. Experimental results from this study indicate that off-design overexpanded nozzle flow was dominated by shock induced boundary layer separation, which was divided into two distinct flow regimes; three- dimensional separation with partial reattachment, and fully detached two-dimensional separation. The test nozzle was observed to go through a marked transition in passing from one regime to the other. In all cases, separation provided a significant increase in static thrust efficiency compared to the ideal prediction. Results indicate that with controlled separation, the entire overexpanded range of nozzle performance would be within 10% of the peak thrust efficiency. By offering savings in weight and complexity over a conventional mechanical exhaust system, this may allow a fixed geometry nozzle to cover an entire flight envelope. The computational simulation was in excellent agreement with experimental data over most of the test range, and did a good job of modeling internal flow and thrust performance. An exception occurred at low nozzle pressure ratios, where the two-dimensional computational model was inconsistent with the three-dimensional separation observed in the experiment. In general, the computation captured the physics of the shock boundary layer interaction and shock induced boundary layer separation in the nozzle, though there were some differences in shock structure compared to experiment. Though minor, these differences could be important for studies involving flow control or thrust vectoring of separated nozzles. Combined with other observations, this indicates that more detailed, three-dimensional computational modeling needs to be conducted to more realistically simulate shock-separated nozzle flows.

Shock unsteadiness in a thrust optimized parabolic nozzle

NASA Astrophysics Data System (ADS)

Verma, S. B.

2009-07-01

This paper discusses the nature of shock unsteadiness, in an overexpanded thrust optimized parabolic nozzle, prevalent in various flow separation modes experienced during start up {(δ P0 /δ t > 0)} and shut down {(δ P0/δ t < 0)} sequences. The results are based on simultaneously acquired data from real-time wall pressure measurements using Kulite pressure transducers, high-speed schlieren (2 kHz) of the exhaust flow-field and from strain-gauges installed on the nozzle bending tube. Shock unsteadiness in the separation region is seen to increase significantly just before the onset of each flow transition, even during steady nozzle operation. The intensity of this measure ( rms level) is seen to be strongly influenced by relative locations of normal and overexpansion shock, the decrease in radial size of re-circulation zone in the back-flow region, and finally, the local nozzle wall contour. During restricted shock separation, the pressure fluctuations in separation region exhibit periodic characteristics rather than the usually observed characteristics of intermittent separation. The possible physical mechanisms responsible for the generation of flow unsteadiness in various separation modes are discussed. The results are from an experimental study conducted in P6.2 cold-gas subscale test facility using a thrust optimized parabolic nozzle of area-ratio 30.

Analytical method for predicting the pressure distribution about a nacelle at transonic speeds

NASA Technical Reports Server (NTRS)

Keith, J. S.; Ferguson, D. R.; Merkle, C. L.; Heck, P. H.; Lahti, D. J.

1973-01-01

The formulation and development of a computer analysis for the calculation of streamlines and pressure distributions around two-dimensional (planar and axisymmetric) isolated nacelles at transonic speeds are described. The computerized flow field analysis is designed to predict the transonic flow around long and short high-bypass-ratio fan duct nacelles with inlet flows and with exhaust flows having appropriate aerothermodynamic properties. The flow field boundaries are located as far upstream and downstream as necessary to obtain minimum disturbances at the boundary. The far-field lateral flow field boundary is analytically defined to exactly represent free-flight conditions or solid wind tunnel wall effects. The inviscid solution technique is based on a Streamtube Curvature Analysis. The computer program utilizes an automatic grid refinement procedure and solves the flow field equations with a matrix relaxation technique. The boundary layer displacement effects and the onset of turbulent separation are included, based on the compressible turbulent boundary layer solution method of Stratford and Beavers and on the turbulent separation prediction method of Stratford.

Meeting in Florida: Using Asymmetric Flow Field-Flow Fractionation (AF4) to Determine C60 Colloidal Size Distributions

EPA Science Inventory

The study of nanomaterials in environmental systems requires robust and specific analytical methods. Analytical methods which discriminate based on particle size and molecular composition are not widely available. Asymmetric Flow Field-Flow Fractionation (AF4) is a separation...

Subsurface Supergranular Vertical Flows as Measured Using Large Distance Separations in Time-Distance Helioseismology

NASA Technical Reports Server (NTRS)

Duvall, Thomas L., Jr.; Hanasoge, S. M.

2012-01-01

As large-distance rays (say, 10 - 24deg) approach the solar surface approximately vertically, travel times measured from surface pairs for these large separations are mostly sensitive to vertical flows, at least for shallow flows within a few Mm of the solar surface. All previous analyses of supergranulation have used smaller separations and have been hampered by the difficulty of separating the horizontal and vertical flow components. We find that the large-separation travel times associated with supergranulation cannot be studied using the standard phase-speed filters of time-distance helioseismology. These filters, whose use is based upon a refractive model of the perturbations, reduce the resultant travel time signal by at least an order of magnitude at some distances. More effective filters are derived. Modeling suggests that the center-annulus travel-time difference [outward-going time minus inward-going time] in the separation range delta= 10 - 24deg is insensitive to the horizontally diverging flow from the centers of the supergranules and should lead to a constant signal from the vertical flow. Our measurement of this quantity, 5.1+/-0.1 seconds, is constant over the distance range. This magnitude of the signal cannot be caused by the level of upflow at cell centers seen at the photosphere of 10 ms(exp-1) extended in depth. It requires the vertical flow to increase with depth. A simple Gaussian model of the increase with depth implies a peak upward flow of 240 ms(exp-1) at a depth of 2.3 Mm and a peak horizontal flow of 700 ms(exp-1) at a depth of 1.6 Mm.

High-performance computing-based exploration of flow control with micro devices.

PubMed

Fujii, Kozo

2014-08-13

The dielectric barrier discharge (DBD) plasma actuator that controls flow separation is one of the promising technologies to realize energy savings and noise reduction of fluid dynamic systems. However, the mechanism for controlling flow separation is not clearly defined, and this lack of knowledge prevents practical use of this technology. Therefore, large-scale computations for the study of the DBD plasma actuator have been conducted using the Japanese Petaflops supercomputer 'K' for three different Reynolds numbers. Numbers of new findings on the control of flow separation by the DBD plasma actuator have been obtained from the simulations, and some of them are presented in this study. Knowledge of suitable device parameters is also obtained. The DBD plasma actuator is clearly shown to be very effective for controlling flow separation at a Reynolds number of around 10(5), and several times larger lift-to-drag ratio can be achieved at higher angles of attack after stall. For higher Reynolds numbers, separated flow is partially controlled. Flow analysis shows key features towards better control. DBD plasma actuators are a promising technology, which could reduce fuel consumption and contribute to a green environment by achieving high aerodynamic performance. The knowledge described above can be obtained only with high-end computers such as the supercomputer 'K'. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Numerical and Experimental study of secondary flows in a rotating two-phase flow: the tea leaf paradox

NASA Astrophysics Data System (ADS)

Calderer, Antoni; Neal, Douglas; Prevost, Richard; Mayrhofer, Arno; Lawrenz, Alan; Foss, John; Sotiropoulos, Fotis

2015-11-01

Secondary flows in a rotating flow in a cylinder, resulting in the so called ``tea leaf paradox'', are fundamental for understanding atmospheric pressure systems, developing techniques for separating red blood cells from the plasma, and even separating coagulated trub in the beer brewing process. We seek to gain deeper insights in this phenomenon by integrating numerical simulations and experiments. We employ the Curvilinear Immersed boundary method (CURVIB) of Calderer et al. (J. Comp. Physics 2014), which is a two-phase flow solver based on the level set method, to simulate rotating free-surface flow in a cylinder partially filled with water as in the tea leave paradox flow. We first demonstrate the validity of the numerical model by simulating a cylinder with a rotating base filled with a single fluid, obtaining results in excellent agreement with available experimental data. Then, we present results for the cylinder case with free surface, investigate the complex formation of secondary flow patterns, and show comparisons with new experimental data for this flow obtained by Lavision. Computational resources were provided by the Minnesota Supercomputing Institute.

Unsteady three-dimensional flow separation

NASA Technical Reports Server (NTRS)

Hui, W. H.

1988-01-01

A concise mathematical framework is constructed to study the topology of steady 3-D separated flows of an incompressible, or a compressible viscous fluid. Flow separation is defined by the existence of a stream surface which intersects with the body surface. The line of separation is itself a skin-friction line. Flow separation is classified as being either regular or singular, depending respectively on whether the line of separation contains only a finite number of singular points or is a singular line of the skin-friction field. The special cases of 2-D and axisymmetric flow separation are shown to be of singular type. In regular separation it is shown that a line of separation originates from a saddle point of separation of the skin-friction field and ends at nodal points of separation. Unsteady flow separation is defined relative to a coordinate system fixed to the body surface. It is shown that separation of an unsteady 3-D incompressible viscous flow at time t, when viewed from such a frame of reference, is topologically the same as that of the fictitious steady flow obtained by freezing the unsteady flow at the instant t. Examples are given showing effects of various forms of flow unsteadiness on flow separation.

Transesterification of rapeseed oil for biodiesel production in trickle-bed reactors packed with heterogeneous Ca/Al composite oxide-based alkaline catalyst.

PubMed

Meng, Yong-Lu; Tian, Song-Jiang; Li, Shu-Fen; Wang, Bo-Yang; Zhang, Min-Hua

2013-05-01

A conventional trickle bed reactor and its modified type both packed with Ca/Al composite oxide-based alkaline catalysts were studied for biodiesel production by transesterification of rapeseed oil and methanol. The effects of the methanol usage and oil flow rate on the FAME yield were investigated under the normal pressure and methanol boiling state. The oil flow rate had a significant effect on the FAME yield for the both reactors. The modified trickle bed reactor kept over 94.5% FAME yield under 0.6 mL/min oil flow rate and 91 mL catalyst bed volume, showing a much higher conversion and operational stability than the conventional type. With the modified trickle bed reactor, both transesterification and methanol separation could be performed simultaneously, and glycerin and methyl esters were separated additionally by gravity separation. Copyright © 2013 Elsevier Ltd. All rights reserved.

Optimal hydrograph separation using a recursive digital filter constrained by chemical mass balance, with application to selected Chesapeake Bay watersheds

USGS Publications Warehouse

Raffensperger, Jeff P.; Baker, Anna C.; Blomquist, Joel D.; Hopple, Jessica A.

2017-06-26

Quantitative estimates of base flow are necessary to address questions concerning the vulnerability and response of the Nation’s water supply to natural and human-induced change in environmental conditions. An objective of the U.S. Geological Survey National Water-Quality Assessment Project is to determine how hydrologic systems are affected by watershed characteristics, including land use, land cover, water use, climate, and natural characteristics (geology, soil type, and topography). An important component of any hydrologic system is base flow, generally described as the part of streamflow that is sustained between precipitation events, fed to stream channels by delayed (usually subsurface) pathways, and more specifically as the volumetric discharge of water, estimated at a measurement site or gage at the watershed scale, which represents groundwater that discharges directly or indirectly to stream reaches and is then routed to the measurement point.Hydrograph separation using a recursive digital filter was applied to 225 sites in the Chesapeake Bay watershed. The recursive digital filter was chosen for the following reasons: it is based in part on the assumption that groundwater acts as a linear reservoir, and so has a physical basis; it has only two adjustable parameters (alpha, obtained directly from recession analysis, and beta, the maximum value of the base-flow index that can be modeled by the filter), which can be determined objectively and with the same physical basis of groundwater reservoir linearity, or that can be optimized by applying a chemical-mass-balance constraint. Base-flow estimates from the recursive digital filter were compared with those from five other hydrograph-separation methods with respect to two metrics: the long-term average fraction of streamflow that is base flow, or base-flow index, and the fraction of days where streamflow is entirely base flow. There was generally good correlation between the methods, with some biased slightly high and some biased slightly low compared to the recursive digital filter. There were notable differences between the days at base flow estimated by the different methods, with the recursive digital filter having a smaller range of values. This was attributed to how the different methods determine cessation of quickflow (the part of streamflow which is not base flow).For 109 Chesapeake Bay watershed sites with available specific conductance data, the parameters of the filter were optimized using a chemical-mass-balance constraint and two different models for the time-dependence of base-flow specific conductance. Sixty-seven models were deemed acceptable and the results compared well with non-optimized results. There are a number of limitations to the optimal hydrograph-separation approach resulting from the assumptions implicit in the conceptual model, the mathematical model, and the approach taken to impose chemical mass balance (including tracer choice). These limitations may be evidenced by poor model results; conversely, poor model fit may provide an indication that two-component separation does not adequately describe the hydrologic system’s runoff response.The results of this study may be used to address a number of questions regarding the role of groundwater in understanding past changes in stream-water quality and forecasting possible future changes, such as the timing and magnitude of land-use and management practice effects on stream and groundwater quality. Ongoing and future modeling efforts may benefit from the estimates of base flow as calibration targets or as a means to filter chemical data to model base-flow loads and trends. Ultimately, base-flow estimation might provide the basis for future work aimed at improving the ability to quantify groundwater discharge, not only at the scale of a gaged watershed, but at the scale of individual reaches as well.

Continuous Flow Deformability-Based Separation of Circulating Tumor Cells Using Microfluidic Ratchets.

PubMed

Park, Emily S; Jin, Chao; Guo, Quan; Ang, Richard R; Duffy, Simon P; Matthews, Kerryn; Azad, Arun; Abdi, Hamidreza; Todenhöfer, Tilman; Bazov, Jenny; Chi, Kim N; Black, Peter C; Ma, Hongshen

2016-04-13

Circulating tumor cells (CTCs) offer tremendous potential for the detection and characterization of cancer. A key challenge for their isolation and subsequent analysis is the extreme rarity of these cells in circulation. Here, a novel label-free method is described to enrich viable CTCs directly from whole blood based on their distinct deformability relative to hematological cells. This mechanism leverages the deformation of single cells through tapered micrometer scale constrictions using oscillatory flow in order to generate a ratcheting effect that produces distinct flow paths for CTCs, leukocytes, and erythrocytes. A label-free separation of circulating tumor cells from whole blood is demonstrated, where target cells can be separated from background cells based on deformability despite their nearly identical size. In doping experiments, this microfluidic device is able to capture >90% of cancer cells from unprocessed whole blood to achieve 10(4) -fold enrichment of target cells relative to leukocytes. In patients with metastatic castration-resistant prostate cancer, where CTCs are not significantly larger than leukocytes, CTCs can be captured based on deformability at 25× greater yield than with the conventional CellSearch system. Finally, the CTCs separated using this approach are collected in suspension and are available for downstream molecular characterization. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Cell design concepts for aqueous lithium-oxygen batteries: A model-based assessment

NASA Astrophysics Data System (ADS)

Grübl, Daniel; Bessler, Wolfgang G.

2015-11-01

Seven cell design concepts for aqueous (alkaline) lithium-oxygen batteries are investigated using a multi-physics continuum model for predicting cell behavior and performance in terms of the specific energy and specific power. Two different silver-based cathode designs (a gas diffusion electrode and a flooded cathode) and three different separator designs (a porous separator, a stirred separator chamber, and a redox-flow separator) are compared. Cathode and separator thicknesses are varied over a wide range (50 μm-20 mm) in order to identify optimum configurations. All designs show a considerable capacity-rate effect due to spatiotemporally inhomogeneous precipitation of solid discharge product LiOH·H2O. In addition, a cell design with flooded cathode and redox-flow separator including oxygen uptake within the external tank is suggested. For this design, the model predicts specific power up to 33 W/kg and specific energy up to 570 Wh/kg (gravimetric values of discharged cell including all cell components and catholyte except housing and piping).

Laminar flow effects in the coil planet centrifuge

NASA Technical Reports Server (NTRS)

Herrmann, F. T.

1984-01-01

The coil planet centrifuge designed by Ito employs flow of a single liquid phase, through a rotating coiled tube in a centrifugal force field, to provide a separation of particles based on sedimentation rates. Mathematical solutions are derived for the linear differential equations governing particle behavior in the coil planet centrifuge device. These solutions are then applied as the basis of a model for optimizing particle separations.

Computational Fluid Mechanics

NASA Technical Reports Server (NTRS)

Hassan, H. A.

1988-01-01

A one-equation turbulence model based on the turbulent kinetic energy equation is presented. The model is motivated by the success of the Johnson-King model and incorporates a number of features uncovered by Simpson's experiments on separated flows. Based on the results obtained, the model duplicates the success of algebraic models in attached flow regions and outperforms the two-equation models in detached flow regions.

Concept of modernization of input device of oil and gas separator

NASA Astrophysics Data System (ADS)

Feodorov, A. B.; Afanasov, V. I.; Miroshnikov, R. S.; Bogachev, V. V.

2017-10-01

The process of defoaming in oil production is discussed. This technology is important in oil and gas fields. Today, the technology of separating the gas fraction is based on chemical catalysis. The use of mechanical technologies improves the economics of the process. Modernization of the separator input device is based on the use of long thin tubes. The chosen length of the tubes is two orders of magnitude larger than the diameter. The separation problem is solved by creating a high centrifugal acceleration. The tubes of the input device are connected in parallel and divide the input stream into several arms. The separated fluid flows are directed tangentially into the working tubes to create a vortex motion. The number of tubes connected in parallel is calculated in accordance with the flow rate of the fluid. The connection of the working tubes to the supply line is made in the form of a flange. This connection allows carrying out maintenance without stopping the flow of fluid. An important feature of this device is its high potential for further modernization. It is concerned with the determination of the parameters of the tubes and the connection geometry in the construction of a single product.

Selective IR multiphoton dissociation of molecules in a pulsed gas-dynamically cooled molecular flow interacting with a solid surface as an alternative to low-energy methods of molecular laser isotope separation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Makarov, G N; Petin, A N

2016-03-31

We report the results of studies on the isotope-selective infrared multiphoton dissociation (IR MFD) of SF{sub 6} and CF{sub 3}I molecules in a pulsed, gas-dynamically cooled molecular flow interacting with a solid surface. The productivity of this method in the conditions of a specific experiment (by the example of SF{sub 6} molecules) is evaluated. A number of low-energy methods of molecular laser isotope separation based on the use of infrared lasers for selective excitation of molecules are analysed and their productivity is estimated. The methods are compared with those of selective dissociation of molecules in the flow interacting with amore » surface. The advantages of this method compared to the low-energy methods of molecular laser isotope separation and the IR MPD method in the unperturbed jets and flows are shown. It is concluded that this method could be a promising alternative to the low-energy methods of molecular laser isotope separation. (laser separation of isotopes)« less

PFB Coal Fired Combined Cycle Development Program. Advanced hot gas cleanup concept evaluation (Task 4. 3). Volume A. Aerodyne cyclone evaluation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This report summarizes the results of testing of a rotary flow cyclone, manufactured by Aerodyne Development Corporation under license by Siemens Kraftwerk Union. This cyclone was selected for evaluation due to the unusually high separative efficiencies claimed by the manufacturer (based on developer data), and relative lack of open literature data. The most significant finding of this work was the observation that electrostatic forces could enhance or, in fact, dominate the separation process. Separative efficiencies, with electrostatic forces present, were found to be substantially independent of flow rate and, by inference, could be independent of unit size. Hence this findingmore » offers a major hope that large cyclones employed in the hot gas cleanup train of the CFCC system may not suffer the performance degradation compared to small cyclones, as projected from conventional inertial theory. The separative efficiencies of the Aerodyne cyclone separator were found from both the cold flow and the hot flow tests to be disappointingly poorer than expectations (in agreement with Westinghouse results), and even poorer than conventional cyclones. (LTN)« less

Measurements in a Transitional Boundary Layer Under Low-Pressure Turbine Airfoil Conditions

NASA Technical Reports Server (NTRS)

Simon, Terrence W.; Qiu, Songgang; Yuan, Kebiao; Ashpis, David (Technical Monitor); Simon, Fred (Technical Monitor)

2000-01-01

This report presents the results of an experimental study of transition from laminar to turbulent flow in boundary layers or in shear layers over separation zones on a convex-curved surface which simulates the suction surface of a low-pressure turbine airfoil. Flows with various free-stream turbulence intensity (FSTI) values (0.5%, 2.5% and 10%), and various Reynolds numbers (50,000, 100,000 200,000 and 300,000) are investigated. Reynold numbers in the present study are based on suction surface length and passage exit mean velocity. Flow separation followed by transition within the separated flow region is observed for the lower-Re cases at each of the FSTI levels. At the highest Reynolds numbers and at elevated FSn, transition of the attached boundary layer begins before separation, and the separation zone is small. Transition proceeds in the shear layer over the separation bubble. For both the transitional boundary layer and the transitional shear layer, mean velocity, turbulence intensity and intermittency (the fraction of the time the flow is turbulent) distributions are presented. The present data are compared to published distribution models for bypass transition, intermittency distribution through transition, transition start position, and transition length. A model developed for transition of separated flows is shown to adequately predict the location of the beginning of transition, for these cases, and a model developed for transitional boundary layer flows seems to adequately predict the path of intermittency through transition when the transition start and end are known. These results are useful for the design of low-pressure turbine stages which are known to operate under conditions replicated by these tests.

Simulations of hypersonic, high-enthalpy separated flow over a 'tick' configuration

NASA Astrophysics Data System (ADS)

Moss, J. N.; O'Byrne, S.; Deepak, N. R.; Gai, S. L.

2012-11-01

The effect of slip is investigated in direct simulation Monte Carlo and Navier-Stokes-based computations of the separated flow between an expansion and a following compression surface, a geometry we call the 'tick' configuration. This configuration has been chosen as a test of separated flow with zero initial boundary layer thickness, a flowfield well suited to Chapman's analytical separated flow theories. The predicted size of the separated region is different for the two codes, although both codes meet their respective particle or grid resolution requirements. Unlike previous comparisons involving cylinder flares or double cones, the separation does not occur in a region of elevated density, and is therefore well suited to the direct simulation Monte Carlo method because the effect of slip at the surface is significant. The reasons for the difference between the two calculations are hypothesized to be a combination of significant rarefaction effects near the expansion surface and the non-zero radius of the leading edge. When the leading edge radius is accounted for, the rarefaction effect at the leading edge is less significant and the behavior of the flowfields predicted by the two methods becomes more similar.

Plume effects on the flow around a blunted cone at hypersonic speeds

NASA Technical Reports Server (NTRS)

Atcliffe, P.; Kumar, D.; Stollery, J. L.

1992-01-01

Tests at M = 8.2 show that a simulated rocket plume at the base of a blunted cone can cause large areas of separated flow, with dramatic effects on the heat transfer rate distribution. The plume was simulated by solid discs of varying sizes or by an annular jet of gas. Flow over the cone without a plume is fully laminar and attached. Using a large disc, the boundary layer is laminar at separation at the test Reynolds number. Transition occurs along the separated shear layer and the boundary layer quickly becomes turbulent. The reduction in heat transfer associated with a laminar separated region is followed by rising values as transition occurs and the heat transfer rates towards the rear of the cone substantially exceed the values obtained without a plume. With the annular jet or a small disc, separation occurs much further aft, so that heat transfer rates at the front of the cone are comparable with those found without a plume. Downstream of separation the shear layer now remains laminar and the heat transfer rates to the surface are significantly lower than the attached flow values.

Enhanced Line Integral Convolution with Flow Feature Detection

NASA Technical Reports Server (NTRS)

Lane, David; Okada, Arthur

1996-01-01

The Line Integral Convolution (LIC) method, which blurs white noise textures along a vector field, is an effective way to visualize overall flow patterns in a 2D domain. The method produces a flow texture image based on the input velocity field defined in the domain. Because of the nature of the algorithm, the texture image tends to be blurry. This sometimes makes it difficult to identify boundaries where flow separation and reattachments occur. We present techniques to enhance LIC texture images and use colored texture images to highlight flow separation and reattachment boundaries. Our techniques have been applied to several flow fields defined in 3D curvilinear multi-block grids and scientists have found the results to be very useful.

Application of mean wall shear stress boundary condition to complex turbulent flows using a wall-modeled large eddy simulation

NASA Astrophysics Data System (ADS)

Cho, Minjeong; Lee, Jungil; Choi, Haecheon

2012-11-01

The mean wall shear stress boundary condition was successfully applied to turbulent channel and boundary flows using large eddy simulation without resolving near-wall region (see Lee, Cho & Choi in this book of abstracts). In the present study, we apply this boundary condition to more complex flows where flow separation and redeveloping flow exist. As a test problem, we consider flow over a backward-facing step at Reh = 22860 based on the step height. Turbulent boundary layer flow at the inlet (Reθ = 1050) is obtained using inflow generation technique by Lund et al. (1998) but with wall shear stress boundary condition. First, we prescribe the mean wall shear stress distribution obtained from DNS (Kim, 2011, Ph.D. Thesis, Stanford U.) as the boundary condition of present simulation. Here we give no-slip boundary condition at flow-reversal region. The present results are in good agreements with the flow statistics by DNS. Currently, a dynamic approach of obtaining mean wall shear stress based on the log-law is being applied to the flow having flow separation and its results will be shown in the presentation. Supported by the WCU and NRF programs.

Cavitating flow during water hammer using a generalized interface vaporous cavitation model

NASA Astrophysics Data System (ADS)

Sadafi, Mohamadhosein; Riasi, Alireza; Nourbakhsh, Seyed Ahmad

2012-10-01

In a transient flow simulation, column separation may occur when the calculated pressure head decreases to the saturated vapor pressure head in a computational grid. Abrupt valve closure or pump failure can result in a fast transient flow with column separation, potentially causing problems such as pipe failure, hydraulic equipment damage, cavitation or corrosion. This paper reports a numerical study of water hammer with column separation in a simple reservoir-pipeline-valve system and pumping station. The governing equations for two-phase transient flow in pipes are solved based on the method of characteristics (MOC) using a generalized interface vaporous cavitating model (GIVCM). The numerical results were compared with the experimental data for validation purposes, and the comparison indicated that the GIVCM describes the experimental results more accurately than the discrete vapor cavity model (DVCM). In particular, the GIVCM correlated better with the experimental data than the DVCM in terms of timing and pressure magnitude. The effects of geometric and hydraulic parameters on flow behavior in a pumping station with column separation were also investigated in this study.

Composite separators and redox flow batteries based on porous separators

DOEpatents

Li, Bin; Wei, Xiaoliang; Luo, Qingtao; Nie, Zimin; Wang, Wei; Sprenkle, Vincent L.

2016-01-12

Composite separators having a porous structure and including acid-stable, hydrophilic, inorganic particles enmeshed in a substantially fully fluorinated polyolefin matrix can be utilized in a number of applications. The inorganic particles can provide hydrophilic characteristics. The pores of the separator result in good selectivity and electrical conductivity. The fluorinated polymeric backbone can result in high chemical stability. Accordingly, one application of the composite separators is in redox flow batteries as low cost membranes. In such applications, the composite separator can also enable additional property-enhancing features compared to ion-exchange membranes. For example, simple capacity control can be achieved through hydraulic pressure by balancing the volumes of electrolyte on each side of the separator. While a porous separator can also allow for volume and pressure regulation, in RFBs that utilize corrosive and/or oxidizing compounds, the composite separators described herein are preferable for their robustness in the presence of such compounds.

Space cryogenics components based on the thermomechanical effect - Vapor-liquid phase separation

NASA Technical Reports Server (NTRS)

Yuan, S. W. K.; Frederking, T. H. K.

1989-01-01

Applications of the thermomechanical effect has been qualified including incorporation in large-scale space systems in the area of vapor-liquid phase separation (VLPS). The theory of the porous-plug phase separator is developed for the limit of a high thermal impedance of the solid-state grains. Extensions of the theory of nonlinear turbulent flow are presented based on experimental results.

Flow visualization for investigating stator losses in a multistage axial compressor

NASA Astrophysics Data System (ADS)

Smith, Natalie R.; Key, Nicole L.

2015-05-01

The methodology and implementation of a powder-paint-based flow visualization technique along with the illuminated flow physics are presented in detail for application in a three-stage axial compressor. While flow visualization often accompanies detailed studies, the turbomachinery literature lacks a comprehensive study which both utilizes flow visualization to interrupt the flow field and explains the intricacies of execution. Lessons learned for obtaining high-quality images of surface flow patterns are discussed in this study. Fluorescent paint is used to provide clear, high-contrast pictures of the recirculation regions on shrouded vane rows. An edge-finding image processing procedure is implemented to provide a quantitative measure of vane-to-vane variability in flow separation, which is approximately 7 % of the suction surface length for Stator 1. Results include images of vane suction side corner separations from all three stages at three loading conditions. Additionally, streakline patterns obtained experimentally are compared with those calculated from computational models. Flow physics associated with vane clocking and increased rotor tip clearance and their implications to stator loss are also investigated with this flow visualization technique. With increased rotor tip clearance, the vane surface flow patterns show a shift to larger separations and more radial flow at the tip. Finally, the effects of instrumentation on the flow field are highlighted.

Predictions of spray combustion interactions

NASA Technical Reports Server (NTRS)

Shuen, J. S.; Solomon, A. S. P.; Faeth, G. M.

1984-01-01

Mean and fluctuating phase velocities; mean particle mass flux; particle size; and mean gas-phase Reynolds stress, composition and temperature were measured in stationary, turbulent, axisymmetric, and flows which conform to the boundary layer approximations while having well-defined initial and boundary conditions in dilute particle-laden jets, nonevaporating sprays, and evaporating sprays injected into a still air environment. Three models of the processes, typical of current practice, were evaluated. The local homogeneous flow and deterministic separated flow models did not provide very satisfactory predictions over the present data base. In contrast, the stochastic separated flow model generally provided good predictions and appears to be an attractive approach for treating nonlinear interphase transport processes in turbulent flows containing particles (drops).

Computational Study of Separating Flow in a Planar Subsonic Diffuser

NASA Technical Reports Server (NTRS)

DalBello, Teryn; Dippold, Vance, III; Georgiadis, Nicholas J.

2005-01-01

A computational study of the separated flow through a 2-D asymmetric subsonic diffuser has been performed. The Wind Computational Fluid Dynamics code is used to predict the separation and reattachment behavior for an incompressible diffuser flow. The diffuser inlet flow is a two-dimensional, turbulent, and fully-developed channel flow with a Reynolds number of 20,000 based on the centerline velocity and the channel height. Wind solutions computed with the Menter SST, Chien k-epsilon, Spalart-Allmaras and Explicit Algebraic Reynolds Stress turbulence models are compared with experimentally measured velocity profiles and skin friction along the upper and lower walls. In addition to the turbulence model study, the effects of grid resolution and use of wall functions were investigated. The grid studies varied the number of grid points across the diffuser and varied the initial wall spacing from y(sup +) = 0.2 to 60. The wall function study assessed the applicability of wall functions for analysis of separated flow. The SST and Explicit Algebraic Stress models provide the best agreement with experimental data, and it is recommended wall functions should only be used with a high level of caution.

Installation Restoration Program. Phase 2. Confirmation/Quantification. Stage 2. Volume 1. Luke Air Force Base, Arizona.

DTIC Science & Technology

1988-06-15

equipped with an oil/water separator. Separated oil would be collected for reuse or proper disposal, and water would be piped to the Sewage Treatment...1940’s. The STP is located on Glendale Avenue, approximately 2 miles east of the Main Base, and adjacent to the Agua Fria River as shown in Figure 1-7...flow. The effluent from the STP is discharged into a canal that flows into lagoons in the dry Agua Fria River bed. The treated efflu- ent is routinely

Thermally conductive porous element-based recuperators

NASA Technical Reports Server (NTRS)

Du, Jian Hua (Inventor); Chow, Louis C (Inventor); Lin, Yeong-Ren (Inventor); Wu, Wei (Inventor); Kapat, Jayanta (Inventor); Notardonato, William U. (Inventor)

2012-01-01

A heat exchanger includes at least one hot fluid flow channel comprising a first plurality of open cell porous elements having first gaps there between for flowing a hot fluid in a flow direction and at least one cold fluid flow channel comprising a second plurality of open cell porous elements having second gaps therebetween for flowing a cold fluid in a countercurrent flow direction relative to the flow direction. The thermal conductivity of the porous elements is at least 10 W/mK. A separation member is interposed between the hot and cold flow channels for isolating flow paths associated these flow channels. The first and second plurality of porous elements at least partially overlap one another to form a plurality of heat transfer pairs which transfer heat from respective ones of the first porous elements to respective ones of the second porous elements through the separation member.

A Gravity-Driven Microfluidic Particle Sorting Device with Hydrodynamic Separation Amplification

PubMed Central

Huh, Dongeun; Bahng, Joong Hwan; Ling, Yibo; Wei, Hsien-Hung; Kripfgans, Oliver D.; Fowlkes, J. Brian; Grotberg, James B.; Takayama, Shuichi

2008-01-01

This paper describes a simple microfluidic sorting system that can perform size-profiling and continuous mass-dependent separation of particles through combined use of gravity (1g) and hydrodynamic flows capable of rapidly amplifying sedimentation-based separation between particles. Operation of the device relies on two microfluidic transport processes: i) initial hydrodynamic focusing of particles in a microchannel oriented parallel to gravity, ii) subsequent sample separation where positional difference between particles with different mass generated by sedimentation is further amplified by hydrodynamic flows whose streamlines gradually widen out due to the geometry of a widening microchannel oriented perpendicular to gravity. The microfluidic sorting device was fabricated in poly(dimethylsiloxane) (PDMS), and hydrodynamic flows in microchannels were driven by gravity without using external pumps. We conducted theoretical and experimental studies on fluid dynamic characteristics of laminar flows in widening microchannels and hydrodynamic amplification of particle separation. Direct trajectory monitoring, collection, and post-analysis of separated particles were performed using polystyrene microbeads with different sizes to demonstrate rapid (< 1 min) and high-purity (> 99.9 %) separation. Finally, we demonstrated biomedical applications of our system by isolating small-sized (diameter < 6 μm) perfluorocarbon liquid droplets from polydisperse droplet emulsions, which is crucial in preparing contrast agents for safe, reliable ultrasound medical imaging, tracers for magnetic resonance imaging, or transpulmonary droplets used in ultrasound-based occlusion therapy for cancer treatment. Our method enables straightforward, rapid real-time size-monitoring and continuous separation of particles in simple stand-alone microfabricated devices without the need for bulky and complex external power sources. We believe that this system will provide a useful tool o separate colloids and particles for various analytical and preparative applications, and may hold 3 potential for separation of cells or development of diagnostic tools requiring point-of-care sample preparation or testing. PMID:17297936

Boundary layer separation on isolated boattail nozzles. M.S. Thesis; [conducted in the Langley 16-foot transonic wind tunnel

NASA Technical Reports Server (NTRS)

Abeyounis, W. K.

1977-01-01

The phenomenon of separated flow on a series of circular-arc afterbodies was investigated using the Langley 16-foot transonic tunnel at free-stream Mach numbers from 0.40 to 0.95 at 0 deg angle of attack. Both high-pressure air and solid circular cylinders with a diameter equal to the nozzle exit diameter were used to simulate jet exhausts. A detailed data base of boundary layer separation locations was obtained using oil-flow techniques. The results indicate that boundary layer separation is most extensive on steep boattails at high Mach numbers.

Heat transfer optimization for air-mist cooling between a stack of parallel plates

NASA Astrophysics Data System (ADS)

Issa, Roy J.

2010-06-01

A theoretical model is developed to predict the upper limit heat transfer between a stack of parallel plates subject to multiphase cooling by air-mist flow. The model predicts the optimal separation distance between the plates based on the development of the boundary layers for small and large separation distances, and for dilute mist conditions. Simulation results show the optimal separation distance to be strongly dependent on the liquid-to-air mass flow rate loading ratio, and reach a limit for a critical loading. For these dilute spray conditions, complete evaporation of the droplets takes place. Simulation results also show the optimal separation distance decreases with the increase in the mist flow rate. The proposed theoretical model shall lead to a better understanding of the design of fins spacing in heat exchangers where multiphase spray cooling is used.

Anisotropy in pair dispersion of inertial particles in turbulent channel flow

NASA Astrophysics Data System (ADS)

Pitton, Enrico; Marchioli, Cristian; Lavezzo, Valentina; Soldati, Alfredo; Toschi, Federico

2012-07-01

The rate at which two particles separate in turbulent flows is of central importance to predict the inhomogeneities of particle spatial distribution and to characterize mixing. Pair separation is analyzed for the specific case of small, inertial particles in turbulent channel flow to examine the role of mean shear and small-scale turbulent velocity fluctuations. To this aim an Eulerian-Lagrangian approach based on pseudo-spectral direct numerical simulation (DNS) of fully developed gas-solid flow at shear Reynolds number Reτ = 150 is used. Pair separation statistics have been computed for particles with different inertia (and for inertialess tracers) released from different regions of the channel. Results confirm that shear-induced effects predominate when the pair separation distance becomes comparable to the largest scale of the flow. Results also reveal the fundamental role played by particles-turbulence interaction at the small scales in triggering separation during the initial stages of pair dispersion. These findings are discussed examining Lagrangian observables, including the mean square separation, which provide prima facie evidence that pair dispersion in non-homogeneous anisotropic turbulence has a superdiffusive nature and may generate non-Gaussian number density distributions of both particles and tracers. These features appear to persist even when the effects of shear dispersion are filtered out, and exhibit strong dependency on particle inertia. Application of present results is discussed in the context of modelling approaches for particle dispersion in wall-bounded turbulent flows.

Intermittent Behavior of the Separated Boundary Layer along the Suction Surface of a Low Pressure Turbine Blade under Periodic Unsteady Flow Conditions

NASA Technical Reports Server (NTRS)

Oeztuerk, B; Schobeiri, M. T.; Ashpis, David E.

2005-01-01

The paper experimentally and theoretically studies the effects of periodic unsteady wake flow and aerodynamic characteristics on boundary layer development, separation and re-attachment along the suction surface of a low pressure turbine blade. The experiments were carried out at Reynolds number of 110,000 (based on suction surface length and exit velocity). For one steady and two different unsteady inlet flow conditions with the corresponding passing frequencies, intermittency behaviors were experimentally and theoretically investigated. The current investigation attempts to extend the intermittency unsteady boundary layer transition model developed in previously to the LPT cases, where separation occurs on the suction surface at a low Reynolds number. The results of the unsteady boundary layer measurements and the intermittency analysis were presented in the ensemble-averaged and contour plot forms. The analysis of the boundary layer experimental data with the flow separation, confirms the universal character of the relative intermittency function which is described by a Gausssian function.

Research on virtual network load balancing based on OpenFlow

NASA Astrophysics Data System (ADS)

Peng, Rong; Ding, Lei

2017-08-01

The Network based on OpenFlow technology separate the control module and data forwarding module. Global deployment of load balancing strategy through network view of control plane is fast and of high efficiency. This paper proposes a Weighted Round-Robin Scheduling algorithm for virtual network and a load balancing plan for server load based on OpenFlow. Load of service nodes and load balancing tasks distribution algorithm will be taken into account.

Fast blood flow monitoring in deep tissues with real-time software correlators

PubMed Central

Wang, Detian; Parthasarathy, Ashwin B.; Baker, Wesley B.; Gannon, Kimberly; Kavuri, Venki; Ko, Tiffany; Schenkel, Steven; Li, Zhe; Li, Zeren; Mullen, Michael T.; Detre, John A.; Yodh, Arjun G.

2016-01-01

We introduce, validate and demonstrate a new software correlator for high-speed measurement of blood flow in deep tissues based on diffuse correlation spectroscopy (DCS). The software correlator scheme employs standard PC-based data acquisition boards to measure temporal intensity autocorrelation functions continuously at 50 – 100 Hz, the fastest blood flow measurements reported with DCS to date. The data streams, obtained in vivo for typical source-detector separations of 2.5 cm, easily resolve pulsatile heart-beat fluctuations in blood flow which were previously considered to be noise. We employ the device to separate tissue blood flow from tissue absorption/scattering dynamics and thereby show that the origin of the pulsatile DCS signal is primarily flow, and we monitor cerebral autoregulation dynamics in healthy volunteers more accurately than with traditional instrumentation as a result of increased data acquisition rates. Finally, we characterize measurement signal-to-noise ratio and identify count rate and averaging parameters needed for optimal performance. PMID:27231588

Knowledge-based modularization and global optimization of artificial neural network models in hydrological forecasting.

PubMed

Corzo, Gerald; Solomatine, Dimitri

2007-05-01

Natural phenomena are multistationary and are composed of a number of interacting processes, so one single model handling all processes often suffers from inaccuracies. A solution is to partition data in relation to such processes using the available domain knowledge or expert judgment, to train separate models for each of the processes, and to merge them in a modular model (committee). In this paper a problem of water flow forecast in watershed hydrology is considered where the flow process can be presented as consisting of two subprocesses -- base flow and excess flow, so that these two processes can be separated. Several approaches to data separation techniques are studied. Two case studies with different forecast horizons are considered. Parameters of the algorithms responsible for data partitioning are optimized using genetic algorithms and global pattern search. It was found that modularization of ANN models using domain knowledge makes models more accurate, if compared with a global model trained on the whole data set, especially when forecast horizon (and hence the complexity of the modelled processes) is increased.

Numerical Simulation of Fluidic Actuators for Flow Control Applications

NASA Technical Reports Server (NTRS)

Vasta, Veer N.; Koklu, Mehti; Wygnanski, Israel L.; Fares, Ehab

2012-01-01

Active flow control technology is finding increasing use in aerospace applications to control flow separation and improve aerodynamic performance. In this paper we examine the characteristics of a class of fluidic actuators that are being considered for active flow control applications for a variety of practical problems. Based on recent experimental work, such actuators have been found to be more efficient for controlling flow separation in terms of mass flow requirements compared to constant blowing and suction or even synthetic jet actuators. The fluidic actuators produce spanwise oscillating jets, and therefore are also known as sweeping jets. The frequency and spanwise sweeping extent depend on the geometric parameters and mass flow rate entering the actuators through the inlet section. The flow physics associated with these actuators is quite complex and not fully understood at this time. The unsteady flow generated by such actuators is simulated using the lattice Boltzmann based solver PowerFLOW R . Computed mean and standard deviation of velocity profiles generated by a family of fluidic actuators in quiescent air are compared with experimental data. Simulated results replicate the experimentally observed trends with parametric variation of geometry and inflow conditions.

Temporal behaviour of a corner separation in a radial vaned diffuser of a centrifugal compressor operating near surge

NASA Astrophysics Data System (ADS)

Marsan, A.; Trébinjac, I.; Coste, S.; Leroy, G.

2013-12-01

The temporal behaviour of a flow separation in the hub-suction side corner of a transonic diffuser is studied thanks to unsteady numerical simulations based on the phase-lagged approach. The validity of the numerical results is confirmed by comparison with experimental unsteady pressure measurements. An analysis of the instantaneous skin-friction pattern and particles trajectories is presented. It highlights the topology of the separation and its temporal behaviour. The major result is that, despite of a highly time-dependent core flow, the separation is found to be a "fixed unsteady separation" characterized by a fixed location of the main saddle of the separation but an extent of the stall region modulated by the pressure waves induced by the impeller-diffuser interaction.

The Baldwin-Lomax model for separated and wake flows using the entropy envelope concept

NASA Technical Reports Server (NTRS)

Brock, J. S.; Ng, W. F.

1992-01-01

Implementation of the Baldwin-Lomax algebraic turbulence model is difficult and ambiguous within flows characterized by strong viscous-inviscid interactions and flow separations. A new method of implementation is proposed which uses an entropy envelope concept and is demonstrated to ensure the proper evaluation of modeling parameters. The method is simple, computationally fast, and applicable to both wake and boundary layer flows. The method is general, making it applicable to any turbulence model which requires the automated determination of the proper maxima of a vorticity-based function. The new method is evalulated within two test cases involving strong viscous-inviscid interaction.

Singular value decomposition of received ultrasound signal to separate tissue, blood flow, and cavitation signals

NASA Astrophysics Data System (ADS)

Ikeda, Hayato; Nagaoka, Ryo; Lafond, Maxime; Yoshizawa, Shin; Iwasaki, Ryosuke; Maeda, Moe; Umemura, Shin-ichiro; Saijo, Yoshifumi

2018-07-01

High-intensity focused ultrasound is a noninvasive treatment applied by externally irradiating ultrasound to the body to coagulate the target tissue thermally. Recently, it has been proposed as a noninvasive treatment for vascular occlusion to replace conventional invasive treatments. Cavitation bubbles generated by the focused ultrasound can accelerate the effect of thermal coagulation. However, the tissues surrounding the target may be damaged by cavitation bubbles generated outside the treatment area. Conventional methods based on Doppler analysis only in the time domain are not suitable for monitoring blood flow in the presence of cavitation. In this study, we proposed a novel filtering method based on the differences in spatiotemporal characteristics, to separate tissue, blood flow, and cavitation by employing singular value decomposition. Signals from cavitation and blood flow were extracted automatically using spatial and temporal covariance matrices.

Enhanced H-filter based on Fåhræus-Lindqvist effect for efficient and robust dialysis without membrane

PubMed Central

Zheng, Wei-Chao; Xie, Rui; He, Li-Qun; Xi, Yue-Heng; Liu, Ying-Mei; Meng, Zhi-Jun; Wang, Wei; Ju, Xiao-Jie; Chen, Gang; Chu, Liang-Yin

2015-01-01

A novel microfluidic device for highly efficient and robust dialysis without membrane is highly desired for the development of portable or wearable microdialyzer. Here we report an enhanced H-filter with pillar array based on Fåhræus-Lindqvist effect (F-L effect) for highly efficient and robust membraneless dialysis of simplified blood for the first time. The H-filter employs two fluids laminarly flowing in the microchannel for continuously membraneless dialysis. With pillar array in the microchannel, the two laminar flows, with one containing blood cells and small molecules and another containing dialyzate solution, can form a cell-free layer at the interface as selective zones for separation. This provides enhanced mixing yet extremely low shear for extraction of small molecules from the blood-cell-containing flow into the dialyzate flow, resulting in robust separation with reduced cell loss and improved efficiency. We demonstrate this by first using Chlorella pyrenoidosa as model cells to quantitatively study the separation performances, and then using simplified human blood for dialysis. The advanced H-filter, with highly efficient and robust performance for membraneless dialysis, shows great potential as promising candidate for rapid blood analysis/separation, and as fundamental structure for portable dialyzer. PMID:26339313

Enhanced H-filter based on Fåhræus-Lindqvist effect for efficient and robust dialysis without membrane.

PubMed

Zheng, Wei-Chao; Xie, Rui; He, Li-Qun; Xi, Yue-Heng; Liu, Ying-Mei; Meng, Zhi-Jun; Wang, Wei; Ju, Xiao-Jie; Chen, Gang; Chu, Liang-Yin

2015-07-01

A novel microfluidic device for highly efficient and robust dialysis without membrane is highly desired for the development of portable or wearable microdialyzer. Here we report an enhanced H-filter with pillar array based on Fåhræus-Lindqvist effect (F-L effect) for highly efficient and robust membraneless dialysis of simplified blood for the first time. The H-filter employs two fluids laminarly flowing in the microchannel for continuously membraneless dialysis. With pillar array in the microchannel, the two laminar flows, with one containing blood cells and small molecules and another containing dialyzate solution, can form a cell-free layer at the interface as selective zones for separation. This provides enhanced mixing yet extremely low shear for extraction of small molecules from the blood-cell-containing flow into the dialyzate flow, resulting in robust separation with reduced cell loss and improved efficiency. We demonstrate this by first using Chlorella pyrenoidosa as model cells to quantitatively study the separation performances, and then using simplified human blood for dialysis. The advanced H-filter, with highly efficient and robust performance for membraneless dialysis, shows great potential as promising candidate for rapid blood analysis/separation, and as fundamental structure for portable dialyzer.

Computational assessment of model-based wave separation using a database of virtual subjects.

PubMed

Hametner, Bernhard; Schneider, Magdalena; Parragh, Stephanie; Wassertheurer, Siegfried

2017-11-07

The quantification of arterial wave reflection is an important area of interest in arterial pulse wave analysis. It can be achieved by wave separation analysis (WSA) if both the aortic pressure waveform and the aortic flow waveform are known. For better applicability, several mathematical models have been established to estimate aortic flow solely based on pressure waveforms. The aim of this study is to investigate and verify the model-based wave separation of the ARCSolver method on virtual pulse wave measurements. The study is based on an open access virtual database generated via simulations. Seven cardiac and arterial parameters were varied within physiological healthy ranges, leading to a total of 3325 virtual healthy subjects. For assessing the model-based ARCSolver method computationally, this method was used to perform WSA based on the aortic root pressure waveforms of the virtual patients. Asa reference, the values of WSA using both the pressure and flow waveforms provided by the virtual database were taken. The investigated parameters showed a good overall agreement between the model-based method and the reference. Mean differences and standard deviations were -0.05±0.02AU for characteristic impedance, -3.93±1.79mmHg for forward pressure amplitude, 1.37±1.56mmHg for backward pressure amplitude and 12.42±4.88% for reflection magnitude. The results indicate that the mathematical blood flow model of the ARCSolver method is a feasible surrogate for a measured flow waveform and provides a reasonable way to assess arterial wave reflection non-invasively in healthy subjects. Copyright © 2017 Elsevier Ltd. All rights reserved.

Navier-Stokes and Euler solutions for lee-side flows over supersonic delta wings. A correlation with experiment

NASA Technical Reports Server (NTRS)

Mcmillin, S. Naomi; Thomas, James L.; Murman, Earll M.

1990-01-01

An Euler flow solver and a thin layer Navier-Stokes flow solver were used to numerically simulate the supersonic leeside flow fields over delta wings which were observed experimentally. Three delta wings with 75, 67.5, and 60 deg leading edge sweeps were computed over an angle-of-attack range of 4 to 20 deg at a Mach number 2.8. The Euler code and Navier-Stokes code predict equally well the primary flow structure where the flow is expected to be separated or attached at the leading edge based on the Stanbrook-Squire boundary. The Navier-Stokes code is capable of predicting both the primary and the secondary flow features for the parameter range investigated. For those flow conditions where the Euler code did not predict the correct type of primary flow structure, the Navier-Stokes code illustrated that the flow structure is sensitive to boundary layer model. In general, the laminar Navier-Stokes solutions agreed better with the experimental data, especially for the lower sweep delta wings. The computational results and a detailed re-examination of the experimental data resulted in a refinement of the flow classifications. This refinement in the flow classification results in the separation bubble with the shock flow type as the intermediate flow pattern between separated and attached flows.

Numerical simulation of the deterministic vector separation of particles flowing over slanted open cavities

NASA Astrophysics Data System (ADS)

Shaqfeh, Eric S. G.; Bernate, Jorge A.; Yang, Mengfei

2016-12-01

Within the past decade, the separation of particles via continuous flow through microfluidic devices has been developed largely through an Edisonian approach whereby devices have been developed based on observation and intuition. This is particularly true in the development of vector chromatography at vanishingly small Reynolds number for non-Brownian particles. Note that this latter phenomenon has its origins in the irreversible forces that are at work in the device, since Stokes flow reversibility typically prohibits their function otherwise. We present a numerical simulation of the vector separation of non-Brownian particles of different sizes and deformabilities in the Stokes flow through channels whose lower surface is composed of slanted cavities. The simulations are designed to understand the physical principles behind the separation as well as to provide design criteria for devices for separating particles in a given size and flexibility range. The numerical simulations are Stokes flow boundary element simulations using techniques defined elsewhere in the literature, but including a close-range repulsive force between the particles and the slanted cavities. We demonstrate that over a range of repulsive force that is comparable to the roughness in the experimental devices, the separation data (particularly in particle size) are predicted quantitatively and are a very weak function of the range of the force. We then vary the geometric parameters of the simulated devices to demonstrate the sensitivity of the separation efficiency to these parameters, thus making design predictions as to which devices are appropriate for separating particles in different size, shape, and deformability ranges.

Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics.

PubMed

Faridi, Muhammad Asim; Ramachandraiah, Harisha; Banerjee, Indradumna; Ardabili, Sahar; Zelenin, Sergey; Russom, Aman

2017-01-04

Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. Here, we report a size based, label-free, bacteria separation from whole blood using elasto-inertial microfluidics. In elasto-inertial microfluidics, the viscoelastic flow enables size based migration of blood cells into a non-Newtonian solution, while smaller bacteria remain in the streamline of the blood sample entrance and can be separated. We first optimized the flow conditions using particles, and show continuous separation of 5 μm particles from 2 μm at a yield of 95% for 5 µm particle and 93% for 2 µm particles at respective outlets. Next, bacteria were continuously separated at an efficiency of 76% from undiluted whole blood sample. We demonstrate separation of bacteria from undiluted while blood using elasto-inertial microfluidics. The label-free, passive bacteria preparation method has a great potential for downstream phenotypic and molecular analysis of bacteria.

Transient Three-Dimensional Analysis of Side Load in Liquid Rocket Engine Nozzles

NASA Technical Reports Server (NTRS)

Wang, Ten-See

2004-01-01

Three-dimensional numerical investigations on the nozzle start-up side load physics were performed. The objective of this study is to identify the three-dimensional side load physics and to compute the associated aerodynamic side load using an anchored computational methodology. The computational methodology is based on an unstructured-grid, and pressure-based computational fluid dynamics formulation, and a simulated inlet condition based on a system calculation. Finite-rate chemistry was used throughout the study so that combustion effect is always included, and the effect of wall cooling on side load physics is studied. The side load physics captured include the afterburning wave, transition from free- shock to restricted-shock separation, and lip Lambda shock oscillation. With the adiabatic nozzle, free-shock separation reappears after the transition from free-shock separation to restricted-shock separation, and the subsequent flow pattern of the simultaneous free-shock and restricted-shock separations creates a very asymmetric Mach disk flow. With the cooled nozzle, the more symmetric restricted-shock separation persisted throughout the start-up transient after the transition, leading to an overall lower side load than that of the adiabatic nozzle. The tepee structures corresponding to the maximum side load were addressed.

Separation control in adverse pressure gradients using high-speed microjets

NASA Astrophysics Data System (ADS)

Kumar, Vikas

Inlets to aircraft propulsion systems must supply flow to the compressor with minimal pressure loss, flow distortion or unsteadiness. Flow separation in internal flows such as inlets and ducts in aircraft propulsion systems and external flows such as over aircraft wings, is undesirable as it reduces the overall system performance. The objective of present study is to understand the nature of separation and more importantly, to explore the applicability of high-speed microjets to actively control this flow separation. The geometry used for this experimental study was a generic backward facing "Stratford Ramp" equipped with arrays of high-speed microjets. The incoming flow was examined over a freestream velocity range of 10-65m/s and at ramp angle in range of 0-10°. It was observed that the flow separates at 30m/s and beyond for all angle of attack. The magnitude and extent of separation bubble increases with increasing adverse pressure gradients and/or increase in free-stream velocity. The separated flow for all the examined conditions was completely attached using suitable array of high-speed microjets. The most notable fact was that elimination of reverse velocity regions was accompanied by a reduction in flow unsteadiness and increased two-dimensionality in the flow. In particular, these gains were achieved with a minimal mass flux, less than 0.2% of the primary flow based on 30% Boundary Layer Ingesting duct. Detailed measurements were obtained to understand the flow control dynamics. The control effectiveness was found to be dependent on the actuation location with respect to separation, jet to cross-flow momentum ratio and the angle at which microjets supply the momentum. It was also determined that the control effect of the microjets, in part, is due to creation of strong stream-wise vortices which enhance the mixing between low-momentum fluid closer to the surface and high-momentum fluid further away from the surface. The penetration depth of microjets was found to be much higher than that of a jet exiting in to uniform cross-flow and correlations were developed to predict this. Subsequently, means for identification of the flow conditions were sought to develop a simple, robust, complete control strategy. It was observed that the flow conditions were very well represented in unsteady surface pressure measurements. The unsteady surface pressure and velocity field were correlated to develop a simple scheme to predict the peak unsteadiness location over the surface. The results from this model and knowledge of microjet in cross flow was used to provide guidelines for an active control strategy. A case study was then undertaken to validate the results obtained using the model. The results show that the model is a good first step towards developing a simple, robust, active-adaptive separation control strategy using microjets.

Effect of Reynolds Number and Periodic Unsteady Wake Flow Condition on Boundary Layer Development, Separation, and Intermittency Behavior Along the Suction Surface of a Low Pressure Turbine Blade

NASA Technical Reports Server (NTRS)

Schobeiri, M. T.; Ozturk, B.; Ashpis, David E.

2007-01-01

The paper experimentally studies the effects of periodic unsteady wake flow and different Reynolds numbers on boundary layer development, separation and re-attachment along the suction surface of a low pressure turbine blade. The experimental investigations were performed on a large scale, subsonic unsteady turbine cascade research facility at Turbomachinery Performance and Flow Research Laboratory (TPFL) of Texas A&M University. The experiments were carried out at Reynolds numbers of 110,000 and 150,000 (based on suction surface length and exit velocity). One steady and two different unsteady inlet flow conditions with the corresponding passing frequencies, wake velocities, and turbulence intensities were investigated. The reduced frequencies chosen cover the operating range of LP turbines. In addition to the unsteady boundary layer measurements, surface pressure measurements were performed. The inception, onset, and the extent of the separation bubble information collected from the pressure measurements were compared with the hot wire measurements. The results presented in ensemble-averaged, and the contour plot forms help to understand the physics of the separation phenomenon under periodic unsteady wake flow and different Reynolds number. It was found that the suction surface displayed a strong separation bubble for these three different reduced frequencies. For each condition, the locations defining the separation bubble were determined carefully analyzing and examining the pressure and mean velocity profile data. The location of the boundary layer separation was dependent of the Reynolds number. It is observed that starting point of the separation bubble and the re-attachment point move further downstream by increasing Reynolds number from 110,000 to 150,000. Also, the size of the separation bubble is smaller when compared to that for Re=110,000.

Aramid Nanofiber Composites for Energy Storage Applications

NASA Astrophysics Data System (ADS)

Tung, Siu on

Lithium ion batteries and non-aqueous redox flow batteries represent two of the most important energy storage technologies to efficient electric vehicles and power grid, which are essential to decreasing U.S. dependence on fossil fuels and sustainable economic growth. Many of the developmental roadblocks for these batteries are related to the separator, an electrically insulating layer between the cathode and anode. Lithium dendrite growth has limited the performance and threatened the safety of lithium ion batteries by piercing the separator and causing internal shorts. In non-aqueous redox flow batteries, active material crossover through microporous separators and the general lack of a suitable ion conducting membrane has led to low operating efficiencies and rapid capacity fade. Developing new separators for these batteries involve the combination of different and sometimes seemingly contradictory properties, such as high ionic conductivity, mechanical stability, thermal stability, chemical stability, and selective permeability. In this dissertation, I present work on composites made from Kevlar-drived aramid nanofibers (ANF) through rational design and fabrication techniques. For lithium ion batteries, a dendrite suppressing layer-by-layer composite of ANF and polyethylene oxide is present with goals of high ionic conductivity, improved safety and thermal stability. For non-aqueous redox flow batteries, a nanoporous ANF separator with surface polyelectrolyte modification is used to achieve high coulombic efficiencies and cycle life in practical flow cells. Finally, manufacturability of ANF based separators is addressed through a prototype machine for continuous ANF separator production and a novel separator coated on anode assembly. In combination, these studies serve as a foundation for addressing the challenges in separator engineering for lithium ion batteries and redox flow batteries.

Effect of Reynolds Number and Periodic Unsteady Wake Flow Condition on Boundary Layer Development, Separation, and Re-attachment along the Suction Surface of a Low Pressure Turbine Blade

NASA Technical Reports Server (NTRS)

Ozturk, B.; Schobeiri, M. T.; Ashpis, David E.

2005-01-01

The paper experimentally studies the effects of periodic unsteady wake flow and different Reynolds numbers on boundary layer development, separation and re-attachment along the suction surface of a low pressure turbine blade. The experimental investigations were performed on a large scale, subsonic unsteady turbine cascade research facility at Turbomachinery Performance and Flow Research Laboratory (TPFL) of Texas A&M University. The experiments were carried out at Reynolds numbers of 110,000 and 150,000 (based on suction surface length and exit velocity). One steady and two different unsteady inlet flow conditions with the corresponding passing frequencies, wake velocities, and turbulence intensities were investigated. The reduced frequencies chosen cover the operating range of LP turbines. In addition to the unsteady boundary layer measurements, surface pressure measurements were performed. The inception, onset, and the extent of the separation bubble information collected from the pressure measurements were compared with the hot wire measurements. The results presented in ensemble-averaged, and the contour plot forms help to understand the physics of the separation phenomenon under periodic unsteady wake flow and different Reynolds number. It was found that the suction surface displayed a strong separation bubble for these three different reduced frequencies. For each condition, the locations defining the separation bubble were determined carefully analyzing and examining the pressure and mean velocity profile data. The location of the boundary layer separation was dependent of the Reynolds number. It is observed that starting point of the separation bubble and the re-attachment point move further downstream by increasing Reynolds number from 110,000 to 150,000. Also, the size of the separation bubble is smaller when compared to that for Re=110,000.

Viscous/potential flow about multi-element two-dimensional and infinite-span swept wings: Theory and experiment

NASA Technical Reports Server (NTRS)

Olson, L. E.; Dvorak, F. A.

1975-01-01

The viscous subsonic flow past two-dimensional and infinite-span swept multi-component airfoils is studied theoretically and experimentally. The computerized analysis is based on iteratively coupled boundary layer and potential flow analysis. The method, which is restricted to flows with only slight separation, gives surface pressure distribution, chordwise and spanwise boundary layer characteristics, lift, drag, and pitching moment for airfoil configurations with up to four elements. Merging confluent boundary layers are treated. Theoretical predictions are compared with an exact theoretical potential flow solution and with experimental measures made in the Ames 40- by 80-Foot Wind Tunnel for both two-dimensional and infinite-span swept wing configurations. Section lift characteristics are accurately predicted for zero and moderate sweep angles where flow separation effects are negligible.

Experimental studies on flow visualization and velocity field of compression ramp with different incoming boundary layers

NASA Astrophysics Data System (ADS)

Wu, Yu; Yi, Shi-He; He, Lin; Chen, Zhi; Zhu, Yang-Zhu

2014-11-01

Experimental studies which focus on flow visualization and the velocity field of a supersonic laminar/turbulent flow over a compression ramp were carried out in a Mach 3.0 wind tunnel. Fine flow structures and velocity field structures were obtained via NPLS (nanoparticle-tracer planar laser scattering) and PIV (particle image velocimetry) techniques, time-averaged flow structures were researched, and spatiotemporal evolutions of transient flow structures were analyzed. The flow visualization results indicated that when the ramp angles were 25°, a typical separation occurred in the laminar flow, some typical flow structures such as shock induced by the boundary layer, separation shock, reversed flow and reattachment shock were visible clearly. While a certain extent separation occurred in turbulent flow, the separation region was much smaller. When the ramp angles were 28°, laminar flow separated further, and the separation region expanded evidently, flow structures in the separation region were complex. While a typical separation occurred in turbulent flow, reversed flow structures were significant, flow structures in the separation region were relatively simple. The experimental results of velocity field were corresponding to flow visualization, and the velocity field structures of both compression ramp flows agreed with the flow structures well. There were three layered structures in the U component velocity, and the V component velocity appeared like an oblique “v”. Some differences between these two compression ramp flows can be observed in the velocity profiles of the shear layer and the shearing intensity.

Proteomic analysis of exosomes from human neural stem cells by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry.

PubMed

Kang, Dukjin; Oh, Sunok; Ahn, Sung-Min; Lee, Bong-Hee; Moon, Myeong Hee

2008-08-01

Exosomes, small membrane vesicles secreted by a multitude of cell types, are involved in a wide range of physiological roles such as intercellular communication, membrane exchange between cells, and degradation as an alternative to lysosomes. Because of the small size of exosomes (30-100 nm) and the limitations of common separation procedures including ultracentrifugation and flow cytometry, size-based fractionation of exosomes has been challenging. In this study, we used flow field-flow fractionation (FlFFF) to fractionate exosomes according to differences in hydrodynamic diameter. The exosome fractions collected from FlFFF runs were examined by transmission electron microscopy (TEM) to morphologically confirm their identification as exosomes. Exosomal lysates of each fraction were digested and analyzed using nanoflow LC-ESI-MS-MS for protein identification. FIFFF, coupled with mass spectrometry, allows nanoscale size-based fractionation of exosomes and is more applicable to primary cells and stem cells since it requires much less starting material than conventional gel-based separation, in-gel digestion and the MS-MS method.

The Azimuth Structure of Nuclear Collisions — I

NASA Astrophysics Data System (ADS)

Trainor, Thomas A.; Kettler, David T.

We describe azimuth structure commonly associated with elliptic and directed flow in the context of 2D angular autocorrelations for the purpose of precise separation of so-called nonflow (mainly minijets) from flow. We extend the Fourier-transform description of azimuth structure to include power spectra and autocorrelations related by the Wiener-Khintchine theorem. We analyze several examples of conventional flow analysis in that context and question the relevance of reaction plane estimation to flow analysis. We introduce the 2D angular autocorrelation with examples from data analysis and describe a simulation exercise which demonstrates precise separation of flow and nonflow using the 2D autocorrelation method. We show that an alternative correlation measure based on Pearson's normalized covariance provides a more intuitive measure of azimuth structure.

Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter.

PubMed

Coelho, Christian; Parot, Jérémie; Gonsior, Michael; Nikolantonaki, Maria; Schmitt-Kopplin, Philippe; Parlanti, Edith; Gougeon, Régis D

2017-04-01

Two analytical separation methods-size-exclusion chromatography and asymmetrical flow field-flow fractionation-were implemented to evaluate the integrity of the colloidal composition of Chardonnay white wine and the impact of pressing and fermentations on the final macromolecular composition. Wine chromophoric colloidal matter, representing UV-visible-absorbing wine macromolecules, was evaluated by optical and structural measurements combined with the description of elution profiles obtained by both separative techniques. The objective of this study was to apply these two types of fractionation on a typical Chardonnay white wine produced in Burgundy and to evaluate how each of them impacted the determination of the macromolecular chromophoric content of wine. UV-visible and fluorescence measurements of collected fractions were successfully applied. An additional proteomic study revealed that grape and microorganism proteins largely impacted the composition of chromophoric colloidal matter of Chardonnay wines. Asymmetrical flow field-flow fractionation appeared to be more reliable and less invasive with respect to the native chemical environment of chromophoric wine macromolecules, and hence is recommended as a tool to fractionate chromophoric colloidal matter in white wines. Graphical Abstract An innovative macromolecular separation method based on Asymmetrical Flow Field-Flow Fractionation was developed to better control colloidal dynamics across Chardonnay white winemaking.

Status of flow separation prediction in liquid propellant rocket nozzles

NASA Technical Reports Server (NTRS)

Schmucker, R. H.

1974-01-01

Flow separation which plays an important role in the design of a rocket engine nozzle is discussed. For a given ambient pressure, the condition of no flow separation limits the area ratio and, therefore, the vacuum performance. Avoidance of performance loss due to area ratio limitation requires a correct prediction of the flow separation conditions. To provide a better understanding of the flow separation process, the principal behavior of flow separation in a supersonic overexpanded rocket nozzle is described. The hot firing separation tests from various sources are summarized, and the applicability and accuracy of the measurements are described. A comparison of the different data points allows an evaluation of the parameters that affect flow separation. The pertinent flow separation predicting methods, which are divided into theoretical and empirical correlations, are summarized and the numerical results are compared with the experimental points.

Development and numerical analysis of low specific speed mixed-flow pump

NASA Astrophysics Data System (ADS)

Li, H. F.; Huo, Y. W.; Pan, Z. B.; Zhou, W. C.; He, M. H.

2012-11-01

With the development of the city, the market of the mixed flow pump with large flux and high head is prospect. The KSB Shanghai Pump Co., LTD decided to develop low speed specific speed mixed flow pump to meet the market requirements. Based on the centrifugal pump and axial flow pump model, aiming at the characteristics of large flux and high head, a new type of guide vane mixed flow pump was designed. The computational fluid dynamics method was adopted to analyze the internal flow of the new type model and predict its performances. The time-averaged Navier-Stokes equations were closed by SST k-ω turbulent model to adapt internal flow of guide vane with larger curvatures. The multi-reference frame(MRF) method was used to deal with the coupling of rotating impeller and static guide vane, and the SIMPLEC method was adopted to achieve the coupling solution of velocity and pressure. The computational results shows that there is great flow impact on the head of vanes at different working conditions, and there is great flow separation at the tailing of the guide vanes at different working conditions, and all will affect the performance of pump. Based on the computational results, optimizations were carried out to decrease the impact on the head of vanes and flow separation at the tailing of the guide vanes. The optimized model was simulated and its performance was predicted. The computational results show that the impact on the head of vanes and the separation at the tailing of the guide vanes disappeared. The high efficiency of the optimized pump is wide, and it fit the original design destination. The newly designed mixed flow pump is now in modeling and its experimental performance will be getting soon.

Prediction of space shuttle fluctuating pressure environments, including rocket plume effects

NASA Technical Reports Server (NTRS)

Plotkin, K. J.; Robertson, J. E.

1973-01-01

Preliminary estimates of space shuttle fluctuating pressure environments have been made based on prediction techniques developed by Wyle Laboratories. Particular emphasis has been given to the transonic speed regime during launch of a parallel-burn space shuttle configuration. A baseline configuration consisting of a lightweight orbiter and monolithic SRB, together with a typical flight trajectory, have been used as models for the predictions. Critical fluctuating pressure environments are predicted at transonic Mach numbers. Comparisons between predicted environments and wind tunnel test results, in general, showed good agreement. Predicted one-third octave band spectra for the above environments were generally one of three types: (1) attached turbulent boundary layer spectra (typically high frequencies); (2) homogeneous separated flow and shock-free interference flow spectra (typically intermediate frequencies); and (3) shock-oscillation and shock-induced interference flow spectra (typically low frequencies). Predictions of plume induced separated flow environments were made. Only the SRB plumes are important, with fluctuating levels comparable to compression-corner induced separated flow shock oscillation.

Application of Synthetic Jets to Reduce Stator Flow Separation in a Low Speed Axial Compressor

NASA Technical Reports Server (NTRS)

Braunscheidel, Edward P.; Culley, Dennis E.; Zaman, Khairul B.M.Q.

2008-01-01

Flow control using synthetic jet injection has been applied in a low speed axial compressor. The synthetic jets were applied from the suction surface of a stator vane via a span-wise row of slots pitched in the streamwise direction. Actuation was provided externally from acoustic drivers coupled to the vane tip via flexible tubing. The acoustic resonance characteristics of the system, and the resultant jet velocities were obtained. The effects on the separated flow field for various jet velocities and frequencies were explored. Total pressure loss reductions across the vane passage were measured. The effect of synthetic jet injection was shown to be comparable to that of pulsatory injection with mass addition for stator vanes which had separated flow. While only a weak dependence of the beneficial effect was noted based on the excitation frequency, a strong dependence on the amplitude was observed at all frequencies.

Calculations of turbulent separated flows

NASA Technical Reports Server (NTRS)

Zhu, J.; Shih, T. H.

1993-01-01

A numerical study of incompressible turbulent separated flows is carried out by using two-equation turbulence models of the K-epsilon type. On the basis of realizability analysis, a new formulation of the eddy-viscosity is proposed which ensures the positiveness of turbulent normal stresses - a realizability condition that most existing two-equation turbulence models are unable to satisfy. The present model is applied to calculate two backward-facing step flows. Calculations with the standard K-epsilon model and a recently developed RNG-based K-epsilon model are also made for comparison. The calculations are performed with a finite-volume method. A second-order accurate differencing scheme and sufficiently fine grids are used to ensure the numerical accuracy of solutions. The calculated results are compared with the experimental data for both mean and turbulent quantities. The comparison shows that the present model performs quite well for separated flows.

Gas separation device based on electrical swing adsorption

DOEpatents

Judkins, Roddie R.; Burchell, Timothy D.

1999-10-26

A method and apparatus for separating one constituent, especially carbon dioxide, from a fluid mixture, such as natural gas. The fluid mixture flows through an adsorbent member having an affinity for molecules of the one constituent, the molecules being adsorbed on the adsorbent member. A voltage is applied to the adsorbent member, the voltage imparting a current flow which causes the molecules of the one constituent to be desorbed from the adsorbent member.

Method of removing an immiscible lubricant from a refrigeration system and apparatus for same

DOEpatents

Spauschus, Hans O.; Starr, Thomas L.

1999-01-01

A method of separating an immiscible lubricant from a liquid refrigerant in a refrigerating system including a compressor, a condenser, an expansion device and an evaporator, wherein the expansion device is connected to the condenser by a liquid refrigerant flow line for liquid refrigerant and immiscible lubricant. The method comprising slowing the rate of flow of the liquid refrigerant and immiscible lubricant between the condenser and the expansion device such that the liquid refrigerant and the immiscible lubricant separate based upon differences in density. The method also comprises collecting the separated immiscible lubricant in a collection chamber in fluid communication with the separated immiscible lubricant. Apparatus for performing the method is also disclosed.

Microfluidic volumetric flow determination using optical coherence tomography speckle: An autocorrelation approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

De Pretto, Lucas R., E-mail: lucas.de.pretto@usp.br; Nogueira, Gesse E. C.; Freitas, Anderson Z.

2016-04-28

Functional modalities of Optical Coherence Tomography (OCT) based on speckle analysis are emerging in the literature. We propose a simple approach to the autocorrelation of OCT signal to enable volumetric flow rate differentiation, based on decorrelation time. Our results show that this technique could distinguish flows separated by 3 μl/min, limited by the acquisition speed of the system. We further perform a B-scan of gradient flow inside a microchannel, enabling the visualization of the drag effect on the walls.

Electrophoretic separator for purifying biologicals, part 1

NASA Technical Reports Server (NTRS)

Mccreight, L. R.

1978-01-01

A program to develop an engineering model of an electrophoretic separator for purifying biologicals is summarized. An extensive mathematical modeling study and numerous ground based tests were included. Focus was placed on developing an actual electrophoretic separator of the continuous flow type, configured and suitable for flight testing as a space processing applications rocket payload.

A simplified sheathless cell separation approach using combined gravitational-sedimentation-based prefocusing and dielectrophoretic separation.

PubMed

Luo, Tao; Fan, Lei; Zeng, Yixiao; Liu, Ya; Chen, Shuxun; Tan, Qiulin; Lam, Raymond H W; Sun, Dong

2018-05-04

Prefocusing of the cell mixture is necessary for achieving a high-efficiency and continuous dielectrophoretic (DEP) cell separation. However, prefocusing through sheath flow requires a complex and tedious peripheral system for multi-channel fluid control, hindering the integration of DEP separation systems with other microfluidic functionalities for comprehensive clinical and biological tasks. This paper presented a simplified sheathless cell separation approach that combines gravitational-sedimentation-based sheathless prefocusing and DEP separation methods. Through gravitational sedimentation in a tubing, which was inserted into the inlet of a microfluidic chip with an adjustable steering angle, the cells were focused into a stream at the upstream region of a microchannel prior to separation. Then, a DEP force was applied at the downstream region of the microchannel for the active separation of the cells. Through this combined strategy, the peripheral system for the sheath flow was no longer required, and thus the integration of cell separation system with additional microfluidic functionalities was facilitated. The proposed sheathless scheme focused the mixture of cells with different sizes and dielectric properties into a stream in a wide range of flow rates without changing the design of the microfluidic chip. The DEP method is a label-free approach that can continuously separate cells on the basis of the sizes or dielectric properties of the cells and thus capable of greatly flexible cell separation. The efficiency of the proposed approach was experimentally assessed according to its performance in the separation of human acute monocytic leukemia THP-1 cells from yeast cells with respect to different sizes and THP-1 cells from human acute myelomonocytic leukemia OCI-AML3 cells with respect to different dielectric properties. The experimental results revealed that the separation efficiency of the method can surpass 90% and thus effective in separating cells on the basis of either size or dielectric property.

COMPILATION OF GROUND WATER MODELS

EPA Science Inventory

The full report presents an overview of currently available computer-based simulation models for ground-water flow, solute and heat transport, and hydrogeochemistry in both porous media and fractured rock. Separate sections address multiphase flow and related chemical species tra...

Numerical aspects and implementation of a two-layer zonal wall model for LES of compressible turbulent flows on unstructured meshes

NASA Astrophysics Data System (ADS)

Park, George Ilhwan; Moin, Parviz

2016-01-01

This paper focuses on numerical and practical aspects associated with a parallel implementation of a two-layer zonal wall model for large-eddy simulation (LES) of compressible wall-bounded turbulent flows on unstructured meshes. A zonal wall model based on the solution of unsteady three-dimensional Reynolds-averaged Navier-Stokes (RANS) equations on a separate near-wall grid is implemented in an unstructured, cell-centered finite-volume LES solver. The main challenge in its implementation is to couple two parallel, unstructured flow solvers for efficient boundary data communication and simultaneous time integrations. A coupling strategy with good load balancing and low processors underutilization is identified. Face mapping and interpolation procedures at the coupling interface are explained in detail. The method of manufactured solution is used for verifying the correct implementation of solver coupling, and parallel performance of the combined wall-modeled LES (WMLES) solver is investigated. The method has successfully been applied to several attached and separated flows, including a transitional flow over a flat plate and a separated flow over an airfoil at an angle of attack.

The application of improved flow diverter for first flush management.

PubMed

Mrowiec, M

2010-01-01

The paper presents the investigations on first flush phenomenon based on the total suspended solids (TSS) concentration measurement during selected rainfalls at central part of Czestochowa (Poland) and also the hydrodynamic model of the catchment. The model allows to present the conception of first flush management using an improved flow diverter Septurn. Flow diverters used in the separate sewer systems create a hybrid system called "semi-separate" sewage system, which allows to treat the first flush volume in the waste water treatment plant (WWTP). Proposed construction of the flow diverter makes possible to capture significant part of the pollutant load (TSS) and simultaneously to reduce volume discharges to WWTPs during wet weather.

Tube Radial Distribution Flow Separation in a Microchannel Using an Ionic Liquid Aqueous Two-Phase System Based on Phase Separation Multi-Phase Flow.

PubMed

Nagatani, Kosuke; Shihata, Yoshinori; Matsushita, Takahiro; Tsukagoshi, Kazuhiko

2016-01-01

Ionic liquid aqueous two-phase systems were delivered into a capillary tube to achieve tube radial distribution flow (TRDF) or annular flow in a microspace. The phase diagram, viscosity of the phases, and TRDF image of the 1-butyl-3-methylimidazolium chloride and NaOH system were examined. The TRDF was formed with inner ionic liquid-rich and outer ionic liquid-poor phases in the capillary tube. The phase configuration was explained using the viscous dissipation principle. We also examined the distribution of rhodamine B in a three-branched microchannel on a microchip with ionic liquid aqueous two-phase systems for the first time.

Detached eddy simulation for turbulent fluid-structure interaction of moving bodies using the constraint-based immersed boundary method

NASA Astrophysics Data System (ADS)

Nangia, Nishant; Bhalla, Amneet P. S.; Griffith, Boyce E.; Patankar, Neelesh A.

2016-11-01

Flows over bodies of industrial importance often contain both an attached boundary layer region near the structure and a region of massively separated flow near its trailing edge. When simulating these flows with turbulence modeling, the Reynolds-averaged Navier-Stokes (RANS) approach is more efficient in the former, whereas large-eddy simulation (LES) is more accurate in the latter. Detached-eddy simulation (DES), based on the Spalart-Allmaras model, is a hybrid method that switches from RANS mode of solution in attached boundary layers to LES in detached flow regions. Simulations of turbulent flows over moving structures on a body-fitted mesh incur an enormous remeshing cost every time step. The constraint-based immersed boundary (cIB) method eliminates this operation by placing the structure on a Cartesian mesh and enforcing a rigidity constraint as an additional forcing in the Navier-Stokes momentum equation. We outline the formulation and development of a parallel DES-cIB method using adaptive mesh refinement. We show preliminary validation results for flows past stationary bodies with both attached and separated boundary layers along with results for turbulent flows past moving bodies. This work is supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1324585.

Experimental Studies of Low-Pressure Turbine Flows and Flow Control

NASA Technical Reports Server (NTRS)

Volino, Ralph J.

2012-01-01

This report summarizes research performed in support of the NASA Glenn Research Center (GRC) Low-Pressure Turbine (LPT) Flow Physics Program. The work was performed experimentally at the U.S. Naval Academy faculties. The geometry corresponded to "Pak B" LPT airfoil. The test section simulated LPT flow in a passage. Three experimental studies were performed: (a) Boundary layer measurements for ten baseline cases under high and low freestream turbulence conditions at five Reynolds numbers of 25,000, 50,000, 100,000, 200,000, and 300,000, based on passage exit velocity and suction surface wetted length; (b) Passive flow control studies with three thicknesses of two-dimensional bars, and two heights of three-dimensional circular cylinders with different spanwise separations, at same flow conditions as the 10 baseline cases; (c) Active flow control with oscillating synthetic (zero net mass flow) vortex generator jets, for one case with low freestream turbulence and a low Reynolds number of 25,000. The Passive flow control was successful at controlling the separation problem at low Reynolds numbers, with varying degrees of success from case to case and varying levels of impact at higher Reynolds numbers. The active flow control successfully eliminated the large separation problem for the low Reynolds number case. Very detailed data was acquired using hot-wire anemometry, including single and two velocity components, integral boundary layer quantities, turbulence statistics and spectra, turbulent shear stresses and their spectra, and intermittency, documenting transition, separation and reattachment. Models were constructed to correlate the results. The report includes a summary of the work performed and reprints of the publications describing the various studies.

Asymmetrical flow field-flow fractionation for human serum albumin based nanoparticle characterisation and a deeper insight into particle formation processes.

PubMed

John, C; Langer, K

2014-06-13

Nanoparticles used as drug delivery systems are of growing interest in the pharmaceutical field. Understanding the behaviour and effects of nanosystems in the human body is dependent on comprehensive characterisation of the systems especially with regard to size and size distribution. Asymmetrical flow field-flow fractionation (AF4) is a promising method for this challenge as this technique enables chromatographic separation of particles and solute molecules according to their respective size. Within this study AF4 was used for the characterisation of human serum albumin (HSA) based nanoparticles. In a first part, the most important aspects of method development like the choice of cross flow rate, focusing and the increase of sample concentration via outlet stream splitting on the sample separation were evaluated. Sample fractionation was controlled by inline-coupling of a dynamic light scattering detector (DLS, Zetasizer) and was confirmed by DLS batch mode measurements. In a second part the applicability of field-flow fractionation for characterisation of the HSA particle formation process by a desolvation method was evaluated. A time dependent particle formation was observed which was controlled by the amount of desolvating agent. Furthermore, field-flow fractionation in combination with in-line dynamic light scattering was used to monitor the increase of particle diameter during PEGylation of the resulting HSA nanoparticles. The separation of nanoparticles from dissolved polyethylene glycol (PEG) could successfully be used for determination of the particles' PEGylation degree. Copyright © 2014 Elsevier B.V. All rights reserved.

Gross separation approaching a blunt trailing edge as the turbulence intensity increases.

PubMed

Scheichl, B

2014-07-28

A novel rational description of incompressible two-dimensional time-mean turbulent boundary layer (BL) flow separating from a bluff body at an arbitrarily large globally formed Reynolds number, Re, is devised. Partly in contrast to and partly complementing previous approaches, it predicts a pronounced delay of massive separation as the turbulence intensity level increases. This is bounded from above by a weakly decaying Re-dependent gauge function (hence, the BL approximation stays intact locally), and thus the finite intensity level characterizing fully developed turbulence. However, it by far exceeds the moderate level found in a preceding study which copes with the associated moderate delay of separation. Thus, the present analysis bridges this self-consistent and another forerunner theory, proposing extremely retarded separation by anticipating a fully attached external potential flow. Specifically, it is shown upon formulation of a respective distinguished limit at which rate the separation point and the attached-flow trailing edge collapse as [Formula: see text] and how on a short streamwise scale the typical small velocity deficit in the core region of the incident BL evolves to a large one. Hence, at its base, the separating velocity profile varies generically with the one-third power of the wall distance, and the classical triple-deck problem describing local viscous-inviscid interaction crucial for moderately retarded separation is superseded by a Rayleigh problem, governing separation of that core layer. Its targeted solution proves vital for understanding the separation process more close to the wall. Most importantly, the analysis does not resort to any specific turbulence closure. A first comparison with the available experimentally found positions of separation for the canonical flow past a circular cylinder is encouraging. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Size-Based Separation of Particles and Cells Utilizing Viscoelastic Effects in Straight Microchannels.

PubMed

Liu, Chao; Xue, Chundong; Chen, Xiaodong; Shan, Lei; Tian, Yu; Hu, Guoqing

2015-06-16

Viscoelasticity-induced particle migration has recently received increasing attention due to its ability to obtain high-quality focusing over a wide range of flow rates. However, its application is limited to low throughput regime since the particles can defocus as flow rate increases. Using an engineered carrier medium with constant and low viscosity and strong elasticity, the sample flow rates are improved to be 1 order of magnitude higher than those in existing studies. Utilizing differential focusing of particles of different sizes, here, we present sheathless particle/cell separation in simple straight microchannels that possess excellent parallelizability for further throughput enhancement. The present method can be implemented over a wide range of particle/cell sizes and flow rates. We successfully separate small particles from larger particles, MCF-7 cells from red blood cells (RBCs), and Escherichia coli (E. coli) bacteria from RBCs in different straight microchannels. The proposed method could broaden the applications of viscoelastic microfluidic devices to particle/cell separation due to the enhanced sample throughput and simple channel design.

On the Physics of Flow Separation Along a Low Pressure Turbine Blade Under Unsteady Flow Conditions

NASA Technical Reports Server (NTRS)

Schobeiri, Meinhard T.; Ozturk, Burak; Ashpis, David E.

2005-01-01

The present study, which is the first of a series of investigations dealing with specific issues of low pressure turbine (LPT) boundary layer aerodynamics, is aimed at providing detailed unsteady boundary flow information to understand the underlying physics of the inception, onset, and extent of the separation zone. A detailed experimental study on the behavior of the separation zone on the suction surface of a highly loaded LPT-blade under periodic unsteady wake flow is presented. Experimental investigations were performed at Texas A&M Turbomachinery Performance and Flow Research Laboratory using a large-scale unsteady turbine cascade research facility with an integrated wake generator and test section unit. To account for a high flow deflection of LPT-cascades at design and off-design operating points, the entire wake generator and test section unit including the traversing system is designed to allow a precise angle adjustment of the cascade relative to the incoming flow. This is done by a hydraulic platform, which simultaneously lifts and rotates the wake generator and test section unit. The unit is then attached to the tunnel exit nozzle with an angular accuracy of better than 0.05 , which is measured electronically. Utilizing a Reynolds number of 110,000 based on the blade suction surface length and the exit velocity, one steady and two different unsteady inlet flow conditions with the corresponding passing frequencies, wake velocities and turbulence intensities are investigated using hot-wire anemometry. In addition to the unsteady boundary layer measurements, blade surface pressure measurements were performed at Re=50,000, 75,000, 100,000, and 125,000 at one steady and two periodic unsteady inlet flow conditions. Detailed unsteady boundary layer measurement identifies the onset and extent of the separation zone as well as its behavior under unsteady wake flow. The results presented in ensemble-averaged and contour plot forms contribute to understanding the physics of the separation phenomenon under periodic unsteady wake flow. Several physical mechanisms are discussed.

Flow processes in overexpanded chemical rocket nozzles. Part 1: Flow separation

NASA Technical Reports Server (NTRS)

Schmucker, R. H.

1984-01-01

An investigation was made of published nozzle flow separation data in order to determine the parameters which affect the separation conditions. A comparison of experimental data with empirical and theoretical separation prediction methods leads to the selection of suitable equations for the separation criterion. The results were used to predict flow separation of the main space shuttle engine.

Flow processes in overexpanded chemical rocket nozzles. Part 1: Flow separation

NASA Technical Reports Server (NTRS)

Schmucker, R. H.

1973-01-01

An investigation was made of published nozzle flow separation data in order to determine the parameters which affect the separation condition. A comparison of experimental data with empirical and theoretical separation prediction methods leads to the selection of suitable equations for the separation criterion. The results were used to predict flow separation of the main space shuttle engine.

Development of a recursion RNG-based turbulence model

NASA Technical Reports Server (NTRS)

Zhou, YE; Vahala, George; Thangam, S.

1993-01-01

Reynolds stress closure models based on the recursion renormalization group theory are developed for the prediction of turbulent separated flows. The proposed model uses a finite wavenumber truncation scheme to account for the spectral distribution of energy. In particular, the model incorporates effects of both local and nonlocal interactions. The nonlocal interactions are shown to yield a contribution identical to that from the epsilon-renormalization group (RNG), while the local interactions introduce higher order dispersive effects. A formal analysis of the model is presented and its ability to accurately predict separated flows is analyzed from a combined theoretical and computational stand point. Turbulent flow past a backward facing step is chosen as a test case and the results obtained based on detailed computations demonstrate that the proposed recursion -RNG model with finite cut-off wavenumber can yield very good predictions for the backstep problem.

A peculiar segmented flow microfluidics for isoquercitrin biosynthesis based on coupling of reaction and separation.

PubMed

Gong, An; Gu, Shuang-Shuang; Wang, Jun; Sheng, Sheng; Wu, Fu-An

2015-10-01

A segmented flow containing a buffer-ionic liquid/solvent in a micro-channel reactor was applied to synthesize isoquercitrin by the hesperidinase-catalyzed selective hydrolysis of rutin, based on a novel system of reaction coupling with separation. Within the developed microchannel reactor with one T-shaped inlet and outlet, the maximum isoquercitrin yield (101.7 ± 2.6%) was achieved in 20 min at 30 °C and 4 μL/min. Compared with a continuous-flow reactor, reaction rate was increased 4-fold due to a glycine-sodium hydroxide:[Bmim][BF4]/glycerol triacetate (1:1, v/v) system that formed a slug flow in microchannel and significantly increased mass transfer rates. The mass transfer coefficient significantly increased and exhibited a linear relationship with the flow rate. Hesperidinase could be efficiently reused at least 5 times, without losing any activity. The bonding mechanism and secondary structure of hesperidinase indicated that hesperidinase had a greater affinity to rutin at a production rate of 4 μL/min in this segmented flow microreactor. Copyright © 2015 Elsevier Ltd. All rights reserved.

Comparison of turbulent separation over a smooth surface and mako shark skin on a NACA 4412 hydrofoil

NASA Astrophysics Data System (ADS)

Smith, Drew; Lang, Amy; Wahidi, Redha

2011-11-01

Shark skin is being investigated as a means of passive flow separation control due to the flexibility and preferential flow direction of the scales covering the skin. In this study, the effect of the scales is observed in a tripped turbulent boundary layer by comparing the flow over a NACA 4412 hydrofoil with a smooth surface to that over the same hydrofoil with samples of mako shark skin affixed to its upper surface. These samples were taken from the flank area of the shark because the scales at that location have been shown to have the greatest angle of erection, and thus the best potential for separation control. All flow data in this study was obtained using Time-Resolved Digital Particle Image Velocimetry and recorded at multiple angles of attack (between 8 and 16 degrees) and two Reynolds numbers. The flow was primarily analyzed by means of the backflow coefficient (a value based on the percentage of time that flow in a region over the hydrofoil is reversed) and the time history of instantaneous flow velocity values at specific points in the boundary layer over the hydrofoil models. Research performed under NSF grant 0932352.

Boundary-Layer Separation Control under Low-Pressure Turbine Airfoil Conditions using Glow-Discharge Plasma Actuators

NASA Technical Reports Server (NTRS)

Hultgren, Lennart S.; Ashpis, David E.

2003-01-01

Modem low-pressure turbines, in general, utilize highly loaded airfoils in an effort to improve efficiency and to lower the number of airfoils needed. Typically, the airfoil boundary layers are turbulent and fully attached at takeoff conditions, whereas a substantial fraction of the boundary layers on the airfoils may be transitional at cruise conditions due to the change of density with altitude. The strong adverse pressure gradients on the suction side of these airfoils can lead to boundary-layer separation at the latter low Reynolds number conditions. Large separation bubbles, particularly those which fail to reattach, cause a significant degradation of engine efficiency. A component efficiency drop of the order 2% may occur between takeoff and cruise conditions for large commercial transport engines and could be as large as 7% for smaller engines at higher altitude. An efficient means of of separation elimination/reduction is, therefore, crucial to improved turbine design. Because the large change in the Reynolds number from takeoff to cruise leads to a distinct change in the airfoil flow physics, a separation control strategy intended for cruise conditions will need to be carefully constructed so as to incur minimum impact/penalty at takeoff. A complicating factor, but also a potential advantage in the quest for an efficient strategy, is the intricate interplay between separation and transition for the situation at hand. Volino gives a comprehensive discussion of several recent studies on transition and separation under low-pressure-turbine conditions, among them one in the present facility. Transition may begin before or after separation, depending on the Reynolds number and other flow conditions. If the transition occurs early in the boundary layer then separation may be reduced or completely eliminated. Transition in the shear layer of a separation bubble can lead to rapid reattachment. This suggests using control mechanisms to trigger and enhance early transition. Gad-el-Hak provides a review of various techniques for flow control in general and Volino discusses recent studies on separation control under low-pressure-turbine conditions utilizing passive as well as active devices. As pointed out by Volino, passive devices optimized for separation control at low Reynolds numbers tend to increase losses at high Reynolds numbers, Active devices have the attractive feature that they can be utilized only in operational regimes where they are needed and when turned off would not affect the flow. The focus in the present paper is an experimental Separation is induced on a flat plate installed in a closed-circuit wind tunnel by a shaped insert on the opposite wall. The flow conditions represent flow over the suction surface of a modem low-pressure-turbine airfoil ('Pak-B'). The Reynolds number, based on wetted plate length and nominal exit velocity, is varied from 50,000 to 300,000, covering cruise to takeoff conditions. Low (0.2%) and high (2.5%) Gee-stream turbulence intensities are set using passive grids. A spanwise-oriented phased-plasma-array actuator, fabricated on a printed circuit board, is surface- flush-mounted upstream of the separation point and can provide forcing in a wide frequency range. Static surface pressure measurements and hot-wire anemometry of the base and controlled flows are performed and indicate that the glow-discharge plasma actuator is an effective device for separation control. of active separation control using glow discharge plasma actuators.

Economic viability and critical influencing factors assessment of black water and grey water source-separation sanitation system.

PubMed

Thibodeau, C; Monette, F; Glaus, M; Laflamme, C B

2011-01-01

The black water and grey water source-separation sanitation system aims at efficient use of energy (biogas), water and nutrients but currently lacks evidence of economic viability to be considered a credible alternative to the conventional system. This study intends to demonstrate economic viability, identify main cost contributors and assess critical influencing factors. A technico-economic model was built based on a new neighbourhood in a Canadian context. Three implementation scales of source-separation system are defined: 500, 5,000 and 50,000 inhabitants. The results show that the source-separation system is 33% to 118% more costly than the conventional system, with the larger cost differential obtained by lower source-separation system implementation scales. A sensitivity analysis demonstrates that vacuum toilet flow reduction from 1.0 to 0.25 L/flush decreases source-separation system cost between 23 and 27%. It also shows that high resource costs can be beneficial or unfavourable to the source-separation system depending on whether the vacuum toilet flow is low or normal. Therefore, the future of this configuration of the source-separation system lies mainly in vacuum toilet flow reduction or the introduction of new efficient effluent volume reduction processes (e.g. reverse osmosis).

Flow-structure interaction simulation of voice production in a canine larynx

NASA Astrophysics Data System (ADS)

Jiang, Weili; Zheng, Xudong; Xue, Qian; Oren, Liran; Khosla, Sid

2017-11-01

Experimental measurements conducted on a hemi-larynx canine vocal fold showed that negative pressures formed in the glottis near the superior surface of the vocal fold in the closing phase even without a supra-glottal vocal tract. It was hypothesized that such negative pressures were due to intraglottal vortices caused by flow separation in a divergent vocal tract during vocal fold closing phase. This work aims to test this hypothesis from the numerical aspect. Flow-structure interaction simulations are performed in realistic canine laryngeal shapes. In the simulations, a sharp interface immersed boundary method based incompressible flow solver is utilized to model the air flow; a finite element based solid mechanics solver is utilized to model the vocal fold vibration. The geometric structure of the vocal fold and vocal tract are based on MRI scans of a mongrel canine. The vocal fold tissue is modeled as transversely isotropic nonlinear materials with a vertical stiffness gradient. Numerical indentation is first performed and compared with the experiment data to obtain the material properties. Simulation setup about the inlet and outlet pressure follows the setup in the experiment. Simulation results including the fundamental frequency, air flow rate, the divergent angle will be compared with the experimental data, providing the validation of the simulation approach. The relationship between flow separation, intra-glottal vortices, divergent angle and flow rate will be comprehensively analyzed.

On the wake flow of asymmetrically beveled trailing edges

NASA Astrophysics Data System (ADS)

Guan, Yaoyi; Pröbsting, Stefan; Stephens, David; Gupta, Abhineet; Morris, Scott C.

2016-05-01

Trailing edge and wake flows are of interest for a wide range of applications. Small changes in the design of asymmetrically beveled or semi-rounded trailing edges can result in significant difference in flow features which are relevant for the aerodynamic performance, flow-induced structural vibration and aerodynamically generated sound. The present study describes in detail the flow field characteristics around a family of asymmetrically beveled trailing edges with an enclosed trailing-edge angle of 25° and variable radius of curvature R. The flow fields over the beveled trailing edges are described using data obtained by particle image velocimetry (PIV) experiments. The flow topology for different trailing edges was found to be strongly dependent on the radius of curvature R, with flow separation occurring further downstream as R increases. This variation in the location of flow separation influences the aerodynamic force coefficients, which were evaluated from the PIV data using a control volume approach. Two-point correlations of the in-plane velocity components are considered to assess the structure in the flow field. The analysis shows large-scale coherent motions in the far wake, which are associated with vortex shedding. The wake thickness parameter yf is confirmed as an appropriate length scale to characterize this large-scale roll-up motion in the wake. The development in the very near wake was found to be critically dependent on R. In addition, high-speed PIV measurements provide insight into the spectral characteristics of the turbulent fluctuations. Based on the time-resolved flow field data, the frequency range associated with the shedding of coherent vortex pairs in the wake is identified. By means of time-correlation of the velocity components, turbulent structures are found to convect from the attached or separated shear layers without distinct separation point into the wake.

Experimental Studies of Low-Pressure Turbine Flows and Flow Control. Streamwise Pressure Profiles and Velocity Profiles

NASA Technical Reports Server (NTRS)

Volino, Ralph

2012-01-01

This report summarizes research performed in support of the NASA Glenn Research Center (GRC) Low-Pressure Turbine (LPT) Flow Physics Program. The work was performed experimentally at the U.S. Naval Academy faculties. The geometry corresponded to "Pak B" LPT airfoil. The test section simulated LPT flow in a passage. Three experimental studies were performed: (a) Boundary layer measurements for ten baseline cases under high and low freestream turbulence conditions at five Reynolds numbers of 25,000, 50,000, 100,000, 200,000, and 300,000, based on passage exit velocity and suction surface wetted length; (b) Passive flow control studies with three thicknesses of two-dimensional bars, and two heights of three-dimensional circular cylinders with different spanwise separations, at same flow conditions as the 10 baseline cases; (c) Active flow control with oscillating synthetic (zero net mass flow) vortex generator jets, for one case with low freestream turbulence and a low Reynolds number of 25,000. The Passive flow control was successful at controlling the separation problem at low Reynolds numbers, with varying degrees of success from case to case and varying levels of impact at higher Reynolds numbers. The active flow control successfully eliminated the large separation problem for the low Reynolds number case. Very detailed data was acquired using hot-wire anemometry, including single and two velocity components, integral boundary layer quantities, turbulence statistics and spectra, turbulent shear stresses and their spectra, and intermittency, documenting transition, separation and reattachment. Models were constructed to correlate the results. The report includes a summary of the work performed and reprints of the publications describing the various studies.This report summarizes research performed in support of the NASA Glenn Research Center (GRC) Low-Pressure Turbine (LPT) Flow Physics Program. The work was performed experimentally at the U.S. Naval Academy faculties. The geometry corresponded to "Pak B" LPT airfoil. The test section simulated LPT flow in a passage. Three experimental studies were performed: (a) Boundary layer measurements for ten baseline cases under high and low freestream turbulence conditions at five Reynolds numbers of 25,000, 50,000, 100,000, 200,000, and 300,000, based on passage exit velocity and suction surface wetted length; (b) Passive flow control studies with three thicknesses of two-dimensional bars, and two heights of three-dimensional circular cylinders with different spanwise separations, at same flow conditions as the 10 baseline cases; (c) Active flow control with oscillating synthetic (zero net mass flow) vortex generator jets, for one case with low freestream turbulence and a low Reynolds number of 25,000. The Passive flow control was successful at controlling the separation problem at low Reynolds numbers, with varying degrees of success from case to case and varying levels of impact at higher Reynolds numbers. The active flow control successfully eliminated the large separation problem for the low Reynolds number case. Very detailed data was acquired using hot-wire anemometry, including single and two velocity components, integral boundary layer quantities, turbulence statistics and spectra, turbulent shear stresses and their spectra, and intermittency, documenting transition, separation and reattachment. Models were constructed to correlate the results. The report includes a summary of the work performed and reprints of the publications describing the various studies. The folders in this supplement contain processed data in ASCII format. Streamwise pressure profiles and velocity profiles are included. The velocity profiles were acquired using single sensor and cross sensor hot-wire probes which were traversed from the wall to the freestream at various streamwise locations. In some of the flow control cases (3D Trips and Jets) profiles were acquired at multiple spanwise locations.

Rapid flow fractionation of particles combining liquid and particulate dielectrophoresis

NASA Technical Reports Server (NTRS)

King, Michael R. (Inventor); Lomakin, Oleg (Inventor); Jones, Thomas B. (Inventor); Ahmed, Rajib (Inventor)

2007-01-01

Rapid, size-based, deposition of particles from liquid suspension is accomplished using a nonuniform electric field created by coplanar microelectrode strips patterned on an insulating substrate. The scheme uses the dielectrophoretic force both to distribute aqueous liquid containing particles and, simultaneously, to separate the particles. Size-based separation is found within nanoliter droplets formed along the structure after voltage removal. Bioparticles or macromolecules of similar size can also be separated based on subtle differences in dielectric property, by controlling the frequency of the AC current supplied to the electrodes.

Method of removing an immiscible lubricant from a refrigeration system and apparatus for same

DOEpatents

Spauschus, H.O.; Starr, T.L.

1999-03-30

A method is described for separating an immiscible lubricant from a liquid refrigerant in a refrigerating system including a compressor, a condenser, an expansion device and an evaporator, wherein the expansion device is connected to the condenser by a liquid refrigerant flow line for liquid refrigerant and immiscible lubricant. The method comprising slowing the rate of flow of the liquid refrigerant and immiscible lubricant between the condenser and the expansion device such that the liquid refrigerant and the immiscible lubricant separate based upon differences in density. The method also comprises collecting the separated immiscible lubricant in a collection chamber in fluid communication with the separated immiscible lubricant. Apparatus for performing the method is also disclosed. 3 figs.

On the Physics of Flow Separation Along a Low Pressure Turbine Blade Under Unsteady Flow Conditions

NASA Technical Reports Server (NTRS)

Schobeiri, Meinhard T.; Ozturk, Burak; Ashpis, David E.

2003-01-01

The present study, which is the first of a series of investigations dealing with specific issues of low pressure turbine (LPT) boundary layer aerodynamics, is aimed at providing detailed unsteady boundary flow information to understand the underlying physics of the inception, onset, and extent of the separation zone. A detailed experimental study on the behavior of the separation zone on the suction surface of a highly loaded LPT-blade under periodic unsteady wake flow is presented. Experimental investigations were performed at Texas A&M Turbomachinery Performance and Flow Research Laboratory using a large-scale unsteady turbine cascade research facility with an integrated wake generator and test section unit. To account for a high flow deflection of LPT-cascades at design and off-design operating points, the entire wake generator and test section unit including the traversing system is designed to allow a precise angle adjustment of the cascade relative to the incoming flow. This is done by a hydraulic platform, which simultaneously lifts and rotates the wake generator and test section unit. The unit is then attached to the tunnel exit nozzle with an angular accuracy of better than 0.05 , which is measured electronically. Utilizing a Reynolds number of 110,000 based on the blade suction surface length and the exit velocity, one steady and two different unsteady inlet flowconditions with the corresponding passing frequencies, wake velocities and turbulence intensities are investigated using hot-wire anemometry. In addition to the unsteady boundary layer measurements, blade surface pressure measurements were performed at Re=50,000, 75,000, 100,000, and 125,000 at one steady and two periodic unsteady inlet flow conditions. Detailed unsteady boundary layer measurement identifies the onset and extent of the separation zone as well as its behavior under unsteady wake flow. The results presented in ensemble-averaged and contour plot forms contribute to understanding the physics of the separation phenomenon under periodic unsteady wake flow. Several physical mechanisms are discussed.

Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics.

PubMed

Sarkar, Aniruddh; Hou, Han Wei; Mahan, Alison E; Han, Jongyoon; Alter, Galit

2016-03-30

Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required for many diagnostic, therapeutic and research applications. Current affinity-based protein or cell separation methods use binary 'bind-elute' separations and are inefficient when applied to the isolation of multiple low-abundance proteins or cell types. We present a method for rapid and multiplexed, yet inexpensive, affinity-based isolation of both proteins and cells, using a size-coded mixture of multiple affinity-capture microbeads and an inertial microfluidic particle sorter device. In a single binding step, different targets-cells or proteins-bind to beads of different sizes, which are then sorted by flowing them through a spiral microfluidic channel. This technique performs continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding. We demonstrate the simultaneous isolation of multiple antibodies from serum and multiple cell types from peripheral blood mononuclear cells or whole blood. We use the technique to isolate low abundance antibodies specific to different HIV antigens and rare HIV-specific cells from blood obtained from HIV+ patients.

Methods And Apparatus For Acoustic Fiber Fractionation

DOEpatents

Brodeur, Pierre

1999-11-09

Methods and apparatus for acoustic fiber fractionation using a plane ultrasonic wave field interacting with water suspended fibers circulating in a channel flow using acoustic radiation forces to separate fibers into two or more fractions based on fiber radius, with applications of the separation concept in the pulp and paper industry. The continuous process relies on the use of a wall-mounted, rectangular cross-section piezoelectric ceramic transducer to selectively deflect flowing fibers as they penetrate the ultrasonic field. The described embodiment uses a transducer frequency of approximately 150 kHz. Depending upon the amount of dissolved gas in water, separation is obtained using a standing or a traveling wave field.

Semi-preparative asymmetrical flow field-flow fractionation: A closer look at channel dimensions and separation performance.

PubMed

Bria, Carmen R M; Skelly, Patrick W; Morse, James R; Schaak, Raymond E; Williams, S Kim Ratanathanawongs

2017-05-26

The design and performance of a semi-preparative asymmetrical flow field-flow fractionation (SP-AF4) channel are investigated with the objective of better understanding and exploiting the relationship between channel dimensions, sample loading, and resolution. Most size-based separations of nanometer and submicrometer particles are currently limited to analytical scale quantities (<100μg). However, there is a strong need to fractionate and collect larger quantities so that fundamental properties of the more narrowly dispersed fractions can be studied using additional characterization methods and for subsequent applications. In this work, dimensions of the spacer that defines the form of SP-AF4 channels are varied and their performances are assessed with respect to sample focusing position and loading. Separations are performed in aqueous and organic carrier fluids. A critical evaluation of channel dimensions showed that increasing the channel breadth is a practical and effective route to maintaining separation resolution while increasing sample loads to milligram quantities. Good size resolution (∼1.0) is achieved for separations of 10mg of 50 and 100nm silica nanoparticles suspended in water and up to 0.6mg of ∼10 to 35nm inorganic hybrid nanoparticles suspended in tetrahydrofuran. This work represents important advances in the understanding of SP-AF4 separations and extends sample loading capacities in both aqueous and organic solvents. Copyright © 2017 Elsevier B.V. All rights reserved.

Continuous cell introduction and rapid dynamic lysis for high-throughput single-cell analysis on microfludic chips with hydrodynamic focusing.

PubMed

Xu, Chun-Xiu; Yin, Xue-Feng

2011-02-04

A chip-based microfluidic system for high-throughput single-cell analysis is described. The system was integrated with continuous introduction of individual cells, rapid dynamic lysis, capillary electrophoretic (CE) separation and laser induced fluorescence (LIF) detection. A cross microfluidic chip with one sheath-flow channel located on each side of the sampling channel was designed. The labeled cells were hydrodynamically focused by sheath-flow streams and sequentially introduced into the cross section of the microchip under hydrostatic pressure generated by adjusting liquid levels in the reservoirs. Combined with the electric field applied on the separation channel, the aligned cells were driven into the separation channel and rapidly lysed within 33ms at the entry of the separation channel by Triton X-100 added in the sheath-flow solution. The maximum rate for introducing individual cells into the separation channel was about 150cells/min. The introduction of sheath-flow streams also significantly reduced the concentration of phosphate-buffered saline (PBS) injected into the separation channel along with single cells, thus reducing Joule heating during electrophoretic separation. The performance of this microfluidic system was evaluated by analysis of reduced glutathione (GSH) and reactive oxygen species (ROS) in single erythrocytes. A throughput of 38cells/min was obtained. The proposed method is simple and robust for high-throughput single-cell analysis, allowing for analysis of cell population with considerable size to generate results with statistical significance. Copyright © 2010 Elsevier B.V. All rights reserved.

Closed-loop separation control over a sharp edge ramp using genetic programming

NASA Astrophysics Data System (ADS)

Debien, Antoine; von Krbek, Kai A. F. F.; Mazellier, Nicolas; Duriez, Thomas; Cordier, Laurent; Noack, Bernd R.; Abel, Markus W.; Kourta, Azeddine

2016-03-01

We experimentally perform open and closed-loop control of a separating turbulent boundary layer downstream from a sharp edge ramp. The turbulent boundary layer just above the separation point has a Reynolds number Re_{θ }≈ 3500 based on momentum thickness. The goal of the control is to mitigate separation and early re-attachment. The forcing employs a spanwise array of active vortex generators. The flow state is monitored with skin-friction sensors downstream of the actuators. The feedback control law is obtained using model-free genetic programming control (GPC) (Gautier et al. in J Fluid Mech 770:442-457, 2015). The resulting flow is assessed using the momentum coefficient, pressure distribution and skin friction over the ramp and stereo PIV. The PIV yields vector field statistics, e.g. shear layer growth, the back-flow area and vortex region. GPC is benchmarked against the best periodic forcing. While open-loop control achieves separation reduction by locking-on the shedding mode, GPC gives rise to similar benefits by accelerating the shear layer growth. Moreover, GPC uses less actuation energy.

Separation and characterization of gold nanoparticle mixtures by flow-field-flow fractionation.

PubMed

Calzolai, Luigi; Gilliland, Douglas; Garcìa, César Pascual; Rossi, François

2011-07-08

We show that using asymmetric flow-field-flow fractionation and UV-vis detector it is possible to separate, characterize, and quantify the correct number size distribution of gold nanoparticle (AuNP) mixtures of various sizes in the 5-60 nm range for which simple dynamic light scattering measurements give misleading information. The size of the collected nanoparticles fractions can be determined both in solution and in the solid state, and their surface chemistry characterized by NMR. This method will find widespread applications both in the process of "size purification" after the synthesis of AuNP and in the identification and characterization of gold-based nanomaterials in consumer products. Copyright © 2011 Elsevier B.V. All rights reserved.

Preventing Bandwidth Abuse at the Router through Sending Rate Estimate-based Active Queue Management

DTIC Science & Technology

2007-06-01

behavior is growing in the Internet. These non-responsive sources can monopolize network bandwidth and starve the “congestion friendly” flows. Without...unnecessarily complex because most of the flows in the Internet are short flows usually termed as “web mice ” [7]. Moreover, having a separate queue for each

Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates

PubMed Central

Tanase, Maya; Zolla, Valerio; Clement, Cristina C; Borghi, Francesco; Urbanska, Aleksandra M; Rodriguez-Navarro, Jose Antonio; Roda, Barbara; Zattoni, Andrea; Reschiglian, Pierluigi; Cuervo, Ana Maria; Santambrogio, Laura

2016-01-01

Herein we describe a protocol that uses hollow-fiber flow field-flow fractionation (FFF) coupled with multiangle light scattering (MALS) for hydrodynamic size-based separation and characterization of complex protein aggregates. The fractionation method, which requires 1.5 h to run, was successfully modified from the analysis of protein aggregates, as found in simple protein mixtures, to complex aggregates, as found in total cell lysates. In contrast to other related methods (filter assay, analytical ultracentrifugation, gel electrophoresis and size-exclusion chromatography), hollow-fiber flow FFF coupled with MALS allows a flow-based fractionation of highly purified protein aggregates and simultaneous measurement of their molecular weight, r.m.s. radius and molecular conformation (e.g., round, rod-shaped, compact or relaxed). The polyethersulfone hollow fibers used, which have a 0.8-mm inner diameter, allow separation of as little as 20 μg of total cell lysates. In addition, the ability to run the samples in different denaturing and nondenaturing buffer allows defining true aggregates from artifacts, which can form during sample preparation. The protocol was set up using Paraquat-induced carbonylation, a model that induces protein aggregation in cultured cells. This technique will advance the biochemical, proteomic and biophysical characterization of molecular-weight aggregates associated with protein mutations, as found in many CNS degenerative diseases, or chronic oxidative stress, as found in aging, and chronic metabolic and inflammatory conditions. PMID:25521790

Interaction of a Synthetic Jet Actuator with a Severely Separated Crossflow

NASA Astrophysics Data System (ADS)

Jansen, Kenneth; Farnsworth, John; Rasquin, Michel; Rathay, Nick; Monastero, Marianne; Amitay, Michael

2017-11-01

A coordinated experimental/computational study of synthetic jet-based flow control on a vertical tail/rudder assembly has been carried out on a 1/19th scale model operating at 30 degree rudder deflection, 0 degree side slip, and 20m/s free-stream flow. Under these conditions a very strong span-wise separated flow develops over the rudder surface for a majority of its span. Twelve synthetic jets were distributed across the span of the vertical tail just upstream of the rudder hinge-line to determine their ability to reduce flow separation and thereby increase the side force production; to extend the rudder effectiveness. Experiments were completed for the baseline case (i.e. no jets blowing) and for cases where 1, 6, and 12 jets were activated. RANS and DDES computations were completed to match these four experiments. While some experimental results for the same geometry have been previously reported, more detailed results concerning the experiments and their comparison to the DDES computations for the baseline and 1 jet active cases are reported here. Specifically, this effort focuses on the near-jet flow and the phase-averaged vortical structures produced by a single jet interacting with a severely separated, turbulent cross-flow. An award of computer time was provided by the INCITE program and the Theta and Aurora ESP through ALCF which is supported by the DOE under Contract DE-AC02-06CH11357.

Hydrodynamic chromatography of macromolecules using polymer monolithic columns.

PubMed

Edam, Rob; Eeltink, Sebastiaan; Vanhoutte, Dominique J D; Kok, Wim Th; Schoenmakers, Peter J

2011-12-02

The selectivity window of size-based separations of macromolecules was tailored by tuning the macropore size of polymer monolithic columns. Monolithic materials with pore sizes ranging between 75 nm and 1.2 μm were prepared in situ in large I.D. columns. The dominant separation mechanism was hydrodynamic chromatography in the flow-through pores. The calibration curves for synthetic polymers matched with the elution behavior by HDC separations in packed columns with 'analyte-to-pore' aspect ratios (λ) up to 0.2. For large-macropore monoliths, a deviation in retention behavior was observed for small polystyrene polymers (M(r)<20 kDa), which may be explained by a combined HDC-SEC mechanism for λ<0.02. The availability of monoliths with very narrow pore sizes allowed investigation of separations at high λ values. For high-molecular weight polymers (M(r)>300,000 Da) confined in narrow channels, the separation strongly depended on flow rate. Flow-rate dependent elution behavior was evaluated by calculation of Deborah numbers and confirmed to be outside the scope of classic shear deformation or slalom chromatography. Shear-induced forces acting on the periphery of coiled polymers in solution may be responsible for flow-rate dependent elution. Copyright © 2011 Elsevier B.V. All rights reserved.

DSMC Simulation of Separated Flows About Flared Bodies at Hypersonic Conditions

NASA Technical Reports Server (NTRS)

Moss, James N.

2000-01-01

This paper describes the results of a numerical study of interacting hypersonic flows at conditions that can be produced in ground-based test facilities. The computations are made with the direct simulation Monte Carlo (DSMC) method of Bird. The focus is on Mach 10 flows about flared axisymmetric configurations, both hollow cylinder flares and double cones. The flow conditions are those for which experiments have been or will be performed in the ONERA R5Ch low-density wind tunnel and the Calspan-University of Buffalo Research Center (CUBRC) Large Energy National Shock (LENS) tunnel. The range of flow conditions, model configurations, and model sizes provides a significant range of shock/shock and shock/boundary layer interactions at low Reynolds number conditions. Results presented will highlight the sensitivity of the calculations to grid resolution, contrast the differences in flow structure for hypersonic cold flows and those of more energetic but still low enthalpy flows, and compare the present results with experimental measurements for surface heating, pressure, and extent of separation.

A study of the second and third order closure models of turbulence for prediction of separated shear flows

NASA Technical Reports Server (NTRS)

Amano, R. S.

1985-01-01

The hybrid model of the Reynolds-stress turbulence closure is tested for the computation of the flows over a step and disk. Here it is attempted to improve the redistributive action of the turbulence energy among the Reynolds stresses. By evaluating the existing models for the pressure-strain correlation, better coefficients are obtained for the prediction of separating shear flows. Furthermore, the diffusion rate of the Reynolds stresses is reevaluated adopting several algebraic correlations for the triple-velocity products. The models of Cormack et al., Daly-Harlow, Hanjalic-Launder, and Shir were tested for the reattaching shear flows. It was generally observed that all these algebraic models give considerably low values of the triple-velocity products. This is attributed to the fact that none of the algebraic models can take the convective effect of the triple-velocity products into account in the separating shear flows, thus resulting in much lower diffusion rate than Reynolds stresses. In order to improve the evaluation of these quantities correction factors are introduced based on the comparison with some experimental data.

Implicit LES of Turbulent, Separated Flow: Wall-Mounted Hump Configuration

NASA Technical Reports Server (NTRS)

Sekhar, Susheel; Mansour, Nagi N.; Caubilla, David Higuera

2015-01-01

Direct simulations (ILES) of turbulent, separated flow over the wall-mounted hump configuration is conducted to investigate the physics of separated flows. A chord-based Reynolds number of Re(sub c) = 47,500 is set up, with a turbulent in flow of Re(sub theta) = 1,400 (theta/c = 3%). FDL3DI, a code that solves the compressible Navier-Stokes equations using high- order compact-difference scheme and filter, with the standard recycling/rescaling method of turbulence generation, is used. Two different configurations of the upper-wall are analyzed, and results are compared with both a higher Re(sub c) (= 936,000, Re(sub theta) = 7,200, theta/c = 0.77%) experiment for major flow features, and RANS (k-omega SST) results. A lower Rec allows for DNS-like mesh resolution, and an adequately wide span. Both ILES and RANS show delayed reattachment compared to experiment, and significantly higher skin friction in the forebody of the hump, as expected. The upper-wall shape influences the C(sub p) distribution only. Results from this study are being used to setup higher Rec (lower theta/c) ILES.

Continuous-flow separation of nanoparticles by electrostatic sieving at a micro-nanofluidic interface.

PubMed

Regtmeier, Jan; Käsewieter, Jörg; Everwand, Martina; Anselmetti, Dario

2011-05-01

Continuous-flow separation of nanoparticles (NPs) (15 and 39 nm) is demonstrated based on electrostatic sieving at a micro-nanofluidic interface. The interface is realized in a poly(dimethylsiloxane) device with a nanoslit of 525 nm laterally spanning the microfluidic channel (aspect ratio of 540:1). Within this nanoslit, the Debye layers overlap and generate an electrostatic sieve. This was exploited to selectively deflect and sort NPs with a sorting purity of up to 97%. Because of the continuous-flow operation, the sample is continuously fed into the device, immediately separated, and the parameters can be adapted in real time. For bioanalytical purposes, we also demonstrate the deflection of proteins (longest axis 6.8 nm). The continuous operation mode and the general applicability of this separation concept make this method a valuable addition to the current Lab-on-a-Chip devices for continuous sorting of NPs and macromolecules. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Optofluidic microscope with 3D spatial resolution.

PubMed

Vig, Asger Laurburg; Marie, Rodolphe; Jensen, Eric; Kristensen, Anders

2010-03-01

This paper reports on-chip based optical detection with three-dimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1 microm , 2.1 microm , 3 microm and 4 microm is determined by the OFM. These results are bench marked against those obtained with a PFF device using conventional fluorescence microscope readout. The size separated microspheres are detected by OFM with an accuracy of

Behavior of a particle-laden flow in a spiral channel

NASA Astrophysics Data System (ADS)

Lee, Sungyon; Stokes, Yvonne; Bertozzi, Andrea L.

2014-04-01

Spiral gravity separators are devices used in mineral processing to separate particles based on their specific gravity or size. The spiral geometry allows for the simultaneous application of gravitational and centripetal forces on the particles, which leads to segregation of particles. However, this segregation mechanism is not fundamentally understood, and the spiral separator literature does not tell a cohesive story either experimentally or theoretically. While experimental results vary depending on the specific spiral separator used, present theoretical works neglect the significant coupling between the particle dynamics and the flow field. Using work on gravity-driven monodisperse slurries on an incline that empirically accounts for this coupling, we consider a monodisperse particle slurry of small depth flowing down a rectangular channel that is helically wound around a vertical axis. We use a thin-film approximation to derive an equilibrium profile for the particle concentration and fluid depth and find that, in the steady state limit, the particles concentrate towards the vertical axis of the helix, leaving a region of clear fluid.

Heterogeneity in the growth hormone pituitary gland system of rats and humans: Implications to microgravity based research

NASA Technical Reports Server (NTRS)

Hymer, W. C.; Grindeland, R.; Hayes, C.; Lanham, J. W.; Cleveland, C.; Todd, P.; Morrison, Dennis R.

1988-01-01

The cell separation techniques of velocity sedimentation, flow cytometry and continuous flow electrophoresis were used to obtain enriched populations of growth hormone (GH) cells. The goal was to isolate a GH cell subpopulation which releases GH molecules which are very high in biological activity, it was important to use a method which was effective in processing large numbers of cells over a short time span. The techniques based on sedimentation are limited by cell density overlaps and streaming. While flow cytometry is useful in the analytical mode for objectively establishing cell purity, the numbers of cells which can be processed in the sort mode are so small as to make this approach ineffective in terms of the long term goals. It was shown that continuous flow electrophoresis systems (CFES) can separate GH cells from other cell types on the basis of differences in surface charge. The bioreactive producers appear to be more electrophoretically mobile than the low producers. Current ground based CFES efforts are hampered by cell clumping in low ionic strength buffers and poor cell recoveries from the CFES device.

Experimental Study of the Effects of Periodic Unsteady Wakes on Flow Separation in Low Pressure Turbines

NASA Technical Reports Server (NTRS)

Ozturk, Burak; Schobeiri, Meinhard T.

2009-01-01

The present study, which is the first of a series of investigations of low pressure turbine (LPT) boundary layer aerodynamics, is aimed at providing detailed unsteady boundary layer flow information to understand the underlying physics of the inception, onset, and extent of the separation zone. A detailed experimental study on the behavior of the separation zone on the suction surface of a highly loaded LPT-blade under periodic unsteady wake flow is presented. Experimental investigations were performed on a large-scale, high-subsonic unsteady turbine cascade research facility with an integrated wake generator and test section unit. Blade Pak B geometry was used in the cascade. The wakes were generated by continuously moving cylindrical bars device. Boundary layer investigations were performed using hot wire anemometry at Reynolds number of 110,000, based on the blade suction surface length and the exit velocity, for one steady and two unsteady inlet flow conditions, with the corresponding passing frequencies, wake velocities, and turbulence intensities. The reduced frequencies cover the entire operation range of LP-turbines. In addition to the unsteady boundary layer measurements, blade surface pressure measurements were performed at Re = 50,000, 75,000, 100,000, 110,000, and 125,000. For each Reynolds number, surface pressure measurements are carried out at one steady and two periodic unsteady inlet flow conditions. Detailed unsteady boundary layer measurement identifies the onset and extension of the separation zone as well as its behavior under unsteady wake flow. The results, presented in ensemble-averaged and contour plot forms, help to understand the physics of the separation phenomenon under periodic unsteady wake flow.

An analytical model for highly seperated flow on airfoils at low speeds

NASA Technical Reports Server (NTRS)

Zunnalt, G. W.; Naik, S. N.

1977-01-01

A computer program was developed to solve the low speed flow around airfoils with highly separated flow. A new flow model included all of the major physical features in the separated region. Flow visualization tests also were made which gave substantiation to the validity of the model. The computation involves the matching of the potential flow, boundary layer and flows in the separated regions. Head's entrainment theory was used for boundary layer calculations and Korst's jet mixing analysis was used in the separated regions. A free stagnation point aft of the airfoil and a standing vortex in the separated region were modelled and computed.

Coil planet centrifugation as a means for small particle separation

NASA Technical Reports Server (NTRS)

Herrmann, F. T.

1983-01-01

The coil planet centrifuge uses a centrifugal force field to provide separation of particles based on differences in sedimentation rates by flow through a rotating coiled tube. Three main separations are considered: (1) single phase fresh sheep and human erythrocytes, (2) single phase fixed heep and human erythrocytes, and (3) electrophoretically enhanced single phase fresh sheep and human erythrocytes.

Separation of red blood cells in deep deterministic lateral displacement devices

NASA Astrophysics Data System (ADS)

Kabacaoglu, Gokberk; Biros, George

2017-11-01

Microfluidic cell separation techniques are of great interest since they help rapid medical diagnoses and tests. Deterministic lateral displacement (DLD) is one of them. A DLD device consists of arrays of pillars. Main flow and alignment of the pillars define two different directions. Size-based separation of rigid spherical particles is possible as they follow one of these directions depending on their sizes. However, the separation of non-spherical deformable particles such as red blood cells (RBCs) is more complicated than that due to their intricate dynamics. We study the separation of RBCs in DLD using an in-house integral equation solver. We systematically investigate the effects of the interior fluid viscosity and the membrane elasticity of an RBC on its behavior. These mechanical properties of a cell determine its deformability, which can be altered by several diseases. We particularly consider deep devices in which an RBC can show rich dynamics such as tank-treading and tumbling. It turns out that strong hydrodynamic lift force moves the tank-treading cells along the pillars and downward force leads the tumbling ones to move with the flow. Thereby, deformability-based separation of RBCs is possible.

Numerical simulation of adverse-pressure-gradient boundary layer with or without roughness

NASA Astrophysics Data System (ADS)

Mottaghian, Pouya; Yuan, Junlin; Piomelli, Ugo

2014-11-01

Large-eddy and direct numerical simulations are carried out on flat-plate boundary layer over smooth and rough surfaces, with adverse pressure gradient.The deceleration is achieved by imposing a wall-normal freestream velocity profile, and is strong enough to cause separation at the wall. The Reynolds number based on momentum thickness and freestream velocity at inlet is 600. Numerical sandgrain roughness is applied based on an immersed boundary method, yielding a flow that is transitionally rough. The turbulence intensity increases before separation, and reaches a higher value for the rough case, indicating stronger mixing. Roughness also causes higher momentum deficit near the wall, leading to earlier separation. This is consistent with previous observation made on rough-wall flow separation over a ramp. In both cases, the turbulent kinetic energy peaks inside the shear layer above the detachment region, with higher values in the rough case; it then decreases approaching the reattachment region. Near the wall inside the separation bubble, the near-zero turbulent intensity indicates that the turbulent structures are lifted up in the separation region. Compared with the smooth case, the shear layer is farther from the wall and the reattachment length is longer on the rough wall.

Sample injection and electrophoretic separation on a simple laminated paper based analytical device.

PubMed

Xu, Chunxiu; Zhong, Minghua; Cai, Longfei; Zheng, Qingyu; Zhang, Xiaojun

2016-02-01

We described a strategy to perform multistep operations on a simple laminated paper-based separation device by using electrokinetic flow to manipulate the fluids. A laminated crossed-channel paper-based separation device was fabricated by cutting a filter paper sheet followed by lamination. Multiple function units including sample loading, sample injection, and electrophoretic separation were integrated on a single paper based analytical device for the first time, by applying potential at different reservoirs for sample, sample waste, buffer, and buffer waste. As a proof-of-concept demonstration, mixed sample solution containing carmine and sunset yellow were loaded in the sampling channel, and then injected into separation channel followed by electrophoretic separation, by adjusting the potentials applied at the four terminals of sampling and separation channel. The effects of buffer pH, buffer concentration, channel width, and separation time on resolution of electrophoretic separation were studied. This strategy may be used to perform multistep operations such as reagent dilution, sample injection, mixing, reaction, and separation on a single microfluidic paper based analytical device, which is very attractive for building micro total analysis systems on microfluidic paper based analytical devices. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Characteristics of Five Ejector Configurations at Free-Stream Mach Numbers from 0 to 2.0

NASA Technical Reports Server (NTRS)

Klann, John L.; Huff, Ronald G.

1959-01-01

Thrust, air-handling, and base-pressure characteristics of five ejector configurations were investigated in the Lewis 8-by 6-foot wind tunnel at free-stream Mach numbers from 0 to 2.0 over ranges of primary-jet pressure ratio up to 24 and corrected secondary weight-flow ratio up to 13 percent. The ejector-shroud geometries varied from convergent to divergent. Base pressure ratio and ejector performance were interrelated by means of an exit-momentum parameter. Correlations, to at least a first approximation, with base pressure ratio, of both internal-ejector-flow separation and external-flow separation over the model boattail were shown. Furthermore, it was shown that magnitudes and exact trends in base pressure ratio depended largely, and in a complicated fashion, on ejector geometry and amount of secondary flow. External-stream effects on ejector jet thrust were determined for a typical schedule of jet-engine pressure ratios. With the exception of the ejector having the largest (1.81) shroud-exit-to primary-diameter ratio, there were no stream effects at Mach numbers from 1.5 to 2.0 and variations from quiescent-air thrust data were less than 2.5 percent at the subsonic speed investigated.

Water outlet control mechanism for fuel cell system operation in variable gravity environments

NASA Technical Reports Server (NTRS)

Vasquez, Arturo (Inventor); McCurdy, Kerri L. (Inventor); Bradley, Karla F. (Inventor)

2007-01-01

A self-regulated water separator provides centrifugal separation of fuel cell product water from oxidant gas. The system uses the flow energy of the fuel cell's two-phase water and oxidant flow stream and a regulated ejector or other reactant circulation pump providing the two-phase fluid flow. The system further uses a means of controlling the water outlet flow rate away from the water separator that uses both the ejector's or reactant pump's supply pressure and a compressibility sensor to provide overall control of separated water flow either back to the separator or away from the separator.

ALESEP. Part 2: A computer program for the analysis of leading edge separation bubbles on infinite swept wings

NASA Technical Reports Server (NTRS)

Davis, R. L.

1986-01-01

A program called ALESEP is presented for the analysis of the inviscid-viscous interaction which occurs due to the presence of a closed laminar-transitional separation bubble on an airfoil or infinite swept wing. The ALESEP code provides an iterative solution of the boundary layer equations expressed in an inverse formulation coupled to a Cauchy integral representation of the inviscid flow. This interaction analysis is treated as a local perturbation to a known solution obtained from a global airfoil analysis; hence, part of the required input to the ALESEP code are the reference displacement thickness and tangential velocity distributions. Special windward differencing may be used in the reversed flow regions of the separation bubble to accurately account for the flow direction in the discretization of the streamwise convection of momentum. The ALESEP code contains a forced transition model based on a streamwise intermittency function, a natural transition model based on a solution of the integral form of the turbulent kinetic energy equation, and an empirical natural transition model.

Application of a Fast Separation Method for Anti-diabetics in Pharmaceuticals Using Monolithic Column: Comparative Study With Silica Based C-18 Particle Packed Column.

PubMed

Hemdan, A; Abdel-Aziz, Omar

2018-04-01

Run time is a predominant factor in HPLC for quality control laboratories especially if there is large number of samples have to be analyzed. Working at high flow rates cannot be attained with silica based particle packed column due to elevated backpressure issues. The use of monolithic column as an alternative to traditional C-18 column was tested for fast separation of pharmaceuticals, where the results were very competitive. The performance comparison of both columns was tested for separation of anti-diabetic combination containing Metformin, Pioglitazone and Glimepiride using Gliclazide as an internal standard. Working at high flow rates with less significant backpressure was obtained with the monolithic column where the run time was reduced from 6 min in traditional column to only 1 min in monolithic column with accepted resolution. The structure of the monolith contains many pores which can adapt the high flow rate of the mobile phase. Moreover, peak symmetry and equilibration time were more efficient with monolithic column.

High-frequency counter-flow plasma synthetic jet actuator and its application in suppression of supersonic flow separation

NASA Astrophysics Data System (ADS)

Wang, Hongyu; Li, Jun; Jin, Di; Tang, Mengxiao; Wu, Yun; Xiao, Lianghua

2018-01-01

We come up with a control strategy for suppression of supersonic flow separation based on high-frequency Counter-flow Plasma Synthetic Jet Actuator (CPSJA). The main purpose of this investigation is to verify if its control authority can be enhanced by the jet/shock interaction. We use a blunt nose to generate a bow shock, a step on a flat plate to introduce a massive separation in a Mach 2 wind tunnel, and the CPSJA to generate Plasma Synthetic Jet (PSJ). In this study, pulsed capacitive discharge is provided for an array of CPSJAs, which makes the actuation (discharge) frequency f1 = 1 kHz, f2 = 2 kHz and f3 = 3 kHz. We use the high-speed schlieren imaging and fast response pressure transducers as well as a numerical simulation to investigate the quiescent PSJ properties, the interaction between the jet and bow shock, and its disturbance effect on the downstream separated region. The schlieren images show that PSJ is characterized by a succession of vortex rings; the jet strength weakens with the increase of frequency. A 4.5 mN jet thrust is found for all the frequencies. The simulation results show that jet/shock interaction produces vorticity in the vortex ring of the jet, enhancing turbulent mixing in PSJ so that a great deal of momentum is produced into the flow. We found the downstream flow is significantly disturbed by the enhanced actuation. Actuation with frequency of f2, f3 which is close to the natural frequency fn of the separation bubble suppresses the separation with the upstream laminar boundary layer being periodically attenuated, which has a better control effect than f1. The control effect is sensitive to the position where PSJ interacts with the shear layer, but the amount of energy deposited in one pulse is not crucial in a separation reduction in the experiment.

Bandwidth turbulence control based on flow community structure in the Internet

NASA Astrophysics Data System (ADS)

Wu, Xiaoyu; Gu, Rentao; Ji, Yuefeng

2016-10-01

Bursty flows vary rapidly in short period of time, and cause fierce bandwidth turbulence in the Internet. In this letter, we model the flow bandwidth turbulence process by constructing a flow interaction network (FIN network), with nodes representing flows and edges denoting bandwidth interactions among them. To restrain the bandwidth turbulence in FIN networks, an immune control strategy based on flow community structure is proposed. Flows in community boundary positions are immunized to cut off the inter-community turbulence spreading. By applying this control strategy in the first- and the second-level flow communities separately, 97.2% flows can effectively avoid bandwidth variations by immunizing 21% flows, and the average bandwidth variation degree reaches near zero. To achieve a similar result, about 70%-90% immune flows are needed with targeted control strategy based on flow degrees and random control strategy. Moreover, simulation results showed that the control effect of the proposed strategy improves significantly if the immune flow number is relatively smaller in each control step.

Annual and average estimates of water-budget components based on hydrograph separation and PRISM precipitation for gaged basins in the Appalachian Plateaus Region, 1900-2011

USGS Publications Warehouse

Nelms, David L.; Messinger, Terence; McCoy, Kurt J.

2015-07-14

As part of the U.S. Geological Survey’s Groundwater Resources Program study of the Appalachian Plateaus aquifers, annual and average estimates of water-budget components based on hydrograph separation and precipitation data from parameter-elevation regressions on independent slopes model (PRISM) were determined at 849 continuous-record streamflow-gaging stations from Mississippi to New York and covered the period of 1900 to 2011. Only complete calendar years (January to December) of streamflow record at each gage were used to determine estimates of base flow, which is that part of streamflow attributed to groundwater discharge; such estimates can serve as a proxy for annual recharge. For each year, estimates of annual base flow, runoff, and base-flow index were determined using computer programs—PART, HYSEP, and BFI—that have automated the separation procedures. These streamflow-hydrograph analysis methods are provided with version 1.0 of the U.S. Geological Survey Groundwater Toolbox, which is a new program that provides graphing, mapping, and analysis capabilities in a Windows environment. Annual values of precipitation were estimated by calculating the average of cell values intercepted by basin boundaries where previously defined in the GAGES–II dataset. Estimates of annual evapotranspiration were then calculated from the difference between precipitation and streamflow.

Topology of Flow Separation on Three-Dimensional Bodies

NASA Technical Reports Server (NTRS)

Chapman, Gary T.; Yates, Leslie A.

1991-01-01

In recent years there has been extensive research on three-dimensional flow separation. There are two different approaches: the phenomenological approach and a mathematical approach using topology. These two approaches are reviewed briefly and the shortcomings of some of the past works are discussed. A comprehensive approach applicable to incompressible and compressible steady-state flows as well as incompressible unsteady flow is then presented. The approach is similar to earlier topological approaches to separation but is more complete and in some cases adds more emphasis to certain points than in the past. To assist in the classification of various types of flow, nomenclature is introduced to describe the skin-friction portraits on the surface. This method of classification is then demonstrated on several categories of flow to illustrate particular points as well as the diversity of flow separation. The categories include attached, two-dimensional separation and three different types of simple, three-dimensional primary separation, secondary separation, and compound separation. Hypothetical experiments are utilized to illustrate the topological terminology and its role in characterizing these flows. These hypothetical experiments use colored oil injected onto the surface at singular points in the skin-friction portrait. Actual flow-visualization information, if available, is used to corroborate the hypothetical examples.

Study of vortex generator influence on the flow in the wake of high-lift system wing

NASA Astrophysics Data System (ADS)

Bragin, N. N.; Ryabov, D. I.; Skomorokhov, S. I.; Slitinskaya, A. Yu.

2016-10-01

Passive vortex generators (VG) are known as one of the ways to improve the flow of the wings and other surfaces in the presence of flow separation. In particular, the VG are installed on the wings and nacelles of many foreign airplanes, including the most recent ones (for example, Boeing 787, Airbus A-350). The principle of the passive VG effects on flow is to transfer the kinetic energy of the external flow separation region by the vortices system arising from the flow VG themselves. For example, by increasing the angle of attack of the wing separation it is highly three-dimensional picture of the flow and sufficiently sensitive to external influences. Thus separated flow can be controlled when using the VG destroy large separation vortices. The VG effectiveness depends on many parameters. This is primarily the relative position of the second harmonic and the separation region on the wing and their size and position relative to each other, the orientation of the second harmonic relative to the local flow direction of the external flow, etc. Obviously, the VG effect will depend essentially on the intensity ratio of the second harmonic vortexes and nature of flow separation in the separation area. In the presence of intense flow separation the effect of conventional VG may be reduced or not occur at all. Until recently, investigations and selection of position of conventional VG were made only experimentally. Currently, the possibilities of calculation methods allow estimating the VG effect on the flow in the separation area. However, due to the phenomenon complexity the accuracy of these calculations is low. The experimental data are required to validate the computational methods, including information not only about the total impact, but also about the flow structure in the separation area. To obtain such information is the subject of this paper. In the test model of high-lift devices swept wing with modern supercritical profile the parametric studies were performed on the VG effects on the flow in the intensive separation zone on flaps. A number of VG types is considered that differ by configuration, size, location in relation to the area of flow separation on the flap, as well as the orientation relative to the incoming flow. The major part of standard of VG positions is investigated. The VG influence on head velocity loss and the characteristics of the amplitude-frequency spectra of pressure fluctuations in the wake of the wing are obtained, as well as the flow spectra are obtained by means of fluorescent mini-tufts.

Numerical analysis of a dielectrophoresis field-flow fractionation device for the separation of multiple cell types.

PubMed

Shamloo, Amir; Kamali, Ali

2017-10-01

In this study, a dielectrophoresis field-flow fractionation device was analyzed using a numerical simulation method and the behaviors of a set of different cells were investigated. By reducing the alternating current frequency of the electrodes from the value used in the original setup configuration and increasing the number of exit channels, total discrimination in cell trajectories and subsequent separation of four cell types were achieved. Cells were differentiated based on their size and dielectric response that are represented in their real part of Clausius-Mossotti factor at different frequencies. A number of novel designs were also proposed based on the original setup configuration. It was seen that by reducing the length of the main channel and the number of electrodes at low frequencies and not changing the inlet flow velocities, cell separation was still achieved successfully, although with a slightly larger electrode voltage. The shorter main channel decreased the residence time for the cells on the chip and also reduced the overall size of the device-these were improvements over the original design. The obtained results can be used to analyze other cell types by knowing their size and dielectric properties to design geometries that can ensure separation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Development of polymeric coatings for control of electro-osmotic flow in ASTP MA-011 electrophoresis technology experiment

NASA Technical Reports Server (NTRS)

Patterson, W. J.

1976-01-01

The development of a methyl cellulose based coating system for control of electro-osmotic flow at the walls of electrophoresis cells is described. Flight electrophoresis columns were coated with this system, resulting in a flight set of six columns. In flight photography of MA-011 electrophoretic separations verified control of electro-osmotic flow.

Role of secondary flows on flow separation induced by shock/boundary layer interaction in supersonic inlets

NASA Astrophysics Data System (ADS)

Morajkar, Rohan

Flow separation in the scramjet air intakes is one of the reasons of failure of these engines which rely on shock waves to achieve flow compression. The shock waves interact with the boundary layers (Shock/ Boundary Layer Interaction or SBLI) on the intake walls inducing adverse pressure gradients causing flow separation. In this experimental study we investigate the role of secondary flows associated with the corners of ducted flows and identify the mechanisms by which they affect flow separation induced by a shock wave interacting with the boundary layers developing along supersonic inlets. The coupling between flow three-dimensionality, shock waves and secondary flows is in fact a key aspect that limits the performance and control of supersonic inlets. The study is conducted at the University of Michigan Glass Supersonic Wind Tunnel (GSWT). This facility replicates some of the features of the three-dimensional (3D) flow-field in a low aspect ratio supersonic inlet. The study uses stereoscopic particle image velocimetry (SPIV) to measure the three-component (3C) velocity field on several orthogonal planes, and thus allows us to identify the length scales of separation, its locations and statistical properties. Furthermore, these measurements allow us to extract the 3D structure of the underlying vortical features, which are important in determining the overall structure of separated regions and their dynamics. The measurements and tools developed are used to study flow fields of three cases: (1) Moderately strong SBLI (Mach 2.75 with 6° deflection), (2) weak SBLI (Mach 2.75 with 4.6° deflection) and (3) secondary corner flows in empty channels. In the configuration of the initial work (moderately strong SBLI), the shock wave system interacts with the boundary layers on the sidewall and the floor of the duct (inlet), thus generating both a swept-shock and an incident-shock interactions. Furthermore, the swept-shock interaction taking place on the sidewalls interacts with the secondary flows in the corners of the tunnel, which are prone to separation. This interaction causes major flow separation on the sidewall as fluid is swept from the sidewall. Flow separation on the floor should be expected given the strength of the SBLI (moderately strong case), but it is instead not observed in the mean flow fields. Our hypothesis is that interacting secondary flows are one of the factors responsible for the sidewall separation and directing the incoming flow towards the center-plane to stabilize and energize the flow on the center of the duct, thus preventing or at least reducing, flow separation on the floor. The secondary flows in an empty tunnel are then investigated to study their evolution and effects on the primary flow field to identify potential separation sites. The results from the empty tunnel experiments are then used to predict locations of flow separations in the moderately strong and weak SBLIs. The predictions were found to be in agreement with the observations.

Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability.

PubMed

Park, Emily S; Duffy, Simon P; Ma, Hongshen

2017-01-01

Circulating tumor cells (CTCs) have been implicated as the seeds of cancer metastasis and therefore have the potential to provide significant prognostic and diagnostic values. Here, we describe a procedure for separating CTCs from whole blood based on size and deformability using the microfluidic ratchet device. This device leverages the ratcheting motion of single cells created as they are deformed through funnel-shaped constrictions using oscillatory flow in order to divert cells based on differences in size and deformability. Subsequent methods for CTC identification and enumeration using immunofluorescence after separation are also described.

A double-gaussian, percentile-based method for estimating maximum blood flow velocity.

PubMed

Marzban, Caren; Illian, Paul R; Morison, David; Mourad, Pierre D

2013-11-01

Transcranial Doppler sonography allows for the estimation of blood flow velocity, whose maximum value, especially at systole, is often of clinical interest. Given that observed values of flow velocity are subject to noise, a useful notion of "maximum" requires a criterion for separating the signal from the noise. All commonly used criteria produce a point estimate (ie, a single value) of maximum flow velocity at any time and therefore convey no information on the distribution or uncertainty of flow velocity. This limitation has clinical consequences especially for patients in vasospasm, whose largest flow velocities can be difficult to measure. Therefore, a method for estimating flow velocity and its uncertainty is desirable. A gaussian mixture model is used to separate the noise from the signal distribution. The time series of a given percentile of the latter, then, provides a flow velocity envelope. This means of estimating the flow velocity envelope naturally allows for displaying several percentiles (e.g., 95th and 99th), thereby conveying uncertainty in the highest flow velocity. Such envelopes were computed for 59 patients and were shown to provide reasonable and useful estimates of the largest flow velocities compared to a standard algorithm. Moreover, we found that the commonly used envelope was generally consistent with the 90th percentile of the signal distribution derived via the gaussian mixture model. Separating the observed distribution of flow velocity into a noise component and a signal component, using a double-gaussian mixture model, allows for the percentiles of the latter to provide meaningful measures of the largest flow velocities and their uncertainty.

Recent theoretical developments and experimental studies pertinent to vortex flow aerodynamics - With a view towards design

NASA Technical Reports Server (NTRS)

Lamar, J. E.; Luckring, J. M.

1978-01-01

A review is presented of recent progress in a research program directed towards the development of an improved vortex-flow technology base. It is pointed out that separation induced vortex-flows from the leading and side edges play an important role in the high angle-of-attack aerodynamic characteristics of a wide range of modern aircraft. In the analysis and design of high-speed aircraft, a detailed knowledge of this type of separation is required, particularly with regard to critical wind loads and the stability and performance at various off-design conditions. A description of analytical methods is presented. The theoretical methods employed are divided into two classes which are dependent upon the underlying aerodynamic assumptions. One conical flow method is considered along with three different nonconical flow methods. Comparisons are conducted between the described methods and available aerodynamic data. Attention is also given to a vortex flow drag study and a vortex flow wing design using suction analogy.

Calculations of separated 3-D flows with a pressure-staggered Navier-Stokes equations solver

NASA Technical Reports Server (NTRS)

Kim, S.-W.

1991-01-01

A Navier-Stokes equations solver based on a pressure correction method with a pressure-staggered mesh and calculations of separated three-dimensional flows are presented. It is shown that the velocity pressure decoupling, which occurs when various pressure correction algorithms are used for pressure-staggered meshes, is caused by the ill-conditioned discrete pressure correction equation. The use of a partial differential equation for the incremental pressure eliminates the velocity pressure decoupling mechanism by itself and yields accurate numerical results. Example flows considered are a three-dimensional lid driven cavity flow and a laminar flow through a 90 degree bend square duct. For the lid driven cavity flow, the present numerical results compare more favorably with the measured data than those obtained using a formally third order accurate quadratic upwind interpolation scheme. For the curved duct flow, the present numerical method yields a grid independent solution with a very small number of grid points. The calculated velocity profiles are in good agreement with the measured data.

Effects of Swirler Shape on Two-Phase Swirling Flow in a Steam Separator

NASA Astrophysics Data System (ADS)

Kataoka, Hironobu; Shinkai, Yusuke; Tomiyama, Akio

Experiments on two-phase swirling flow in a separator are carried out using several swirlers having different vane angles, different hub diameters and different number of vanes to seek a way for improving steam separators of uprated boiling water reactors. Ratios of the separated liquid flow rate to the total liquid flow rate, flow patterns, liquid film thicknesses and pressure drops are measured to examine the effects of swirler shape on air-water two-phase swirling annular flows in a one-fifth scale model of the separator. As a result, the following conclusions are obtained for the tested swirlers: (1) swirler shape scarcely affects the pressure drop in the barrel of the separator, (2) decreasing the vane angle is an effective way for reducing the pressure drop in the diffuser of the separator, and (3) the film thickness at the inlet of the pick-off-ring of the separator is not sensitive to swirler shape, which explains the reason why the separator performance does not depend on swirler shape.

Investigation of Flow Separation in a Transonic-fan Linear Cascade Using Visualization Methods

NASA Technical Reports Server (NTRS)

Lepicovsky, Jan; Chima, Rodrick V.; Jett, Thomas A.; Bencic, Timothy J.; Weiland, Kenneth E.

2000-01-01

An extensive study into the nature of the separated flows on the suction side of modem transonic fan airfoils at high incidence is described in the paper. Suction surface.flow separation is an important flow characteristic that may significantly contribute to stall flutter in transonic fans. Flutter in axial turbomachines is a highly undesirable and dangerous self-excited mode of blade oscillations that can result in high cycle fatigue blade failure. The study basically focused on two visualization techniques: surface flow visualization using dye oils, and schlieren (and shadowgraph) flow visualization. The following key observations were made during the study. For subsonic inlet flow, the flow on the suction side of the blade is separated over a large portion of the blade, and the separated area increases with increasing inlet Mach number. For the supersonic inlet flow condition, the flow is attached from the leading edge up to the point where a bow shock from the upper neighboring blade hits the blade surface. Low cascade solidity, for the subsonic inlet flow, results in an increased area of separated flow. For supersonic flow conditions, a low solidity results in an improvement in flow over the suction surface. Finally, computational results modeling the transonic cascade flowfield illustrate our ability to simulate these flows numerically.

Time-of-flight dependency on transducer separation distance in a reflective-path guided-wave ultrasonic flow meter at zero flow conditions.

PubMed

Aanes, Magne; Kippersund, Remi Andre; Lohne, Kjetil Daae; Frøysa, Kjell-Eivind; Lunde, Per

2017-08-01

Transit-time flow meters based on guided ultrasonic wave propagation in the pipe spool have several advantages compared to traditional inline ultrasonic flow metering. The extended interrogation field, obtained by continuous leakage from guided waves traveling in the pipe wall, increases robustness toward entrained particles or gas in the flow. In reflective-path guided-wave ultrasonic flow meters (GW-UFMs), the flow equations are derived from signals propagating solely in the pipe wall and from signals passing twice through the fluid. In addition to the time-of-flight (TOF) through the fluid, the fluid path experiences an additional time delay upon reflection at the opposite pipe wall due to specular and non-specular reflections. The present work investigates the influence of these reflections on the TOF in a reflective-path GW-UFM as a function of transducer separation distance at zero flow conditions. Two models are used to describe the signal propagation through the system: (i) a transient full-wave finite element model, and (ii) a combined plane-wave and ray-tracing model. The study shows that a range-dependent time delay is associated with the reflection of the fluid path, introducing transmitter-receiver distance dependence. Based on these results, the applicability of the flow equations derived using model (ii) is discussed.

The role of RhD agglutination for the detection of weak D red cells by anti-D flow cytometry.

PubMed

Grey, D E; Davies, J I; Connolly, M; Fong, E A; Erber, W N

2005-04-01

Anti-D flow cytometry is an accurate method for quantifying feto-maternal haemorrhage (FMH). However, weak D red cells with <1000 RhD sites are not detectable using this methodology but are immunogenic. As quantitation of RhD sites is not practical, an alternative approach is required to identify those weak D fetal red cells where anti-D flow cytometry is inappropriate. We describe a simple algorithm based on RhD agglutination and flow cytometry peak separation. All weak D (n = 34) gave weak agglutination with RUM-1 on immediate spin (grading

Experimental Investigations on the Surface-Driven Capillary Flow of Aqueous Microparticle Suspensions in the Microfluidic Laboratory-On Systems

NASA Astrophysics Data System (ADS)

Mukhopadhyay, Subhadeep

In this work, total 1592 individual leakage-free polymethylmethacrylate (PMMA) microfluidic devices as laboratory-on-a-chip systems are fabricated by maskless lithography, hot embossing lithography, and direct bonding technique. Total 1094 individual Audio Video Interleave Files as experimental outputs related to the surface-driven capillary flow have been recorded and analyzed. The influence of effective viscosity, effect of surface wettability, effect of channel aspect ratio, and effect of centrifugal force on the surface-driven microfluidic flow of aqueous microparticle suspensions have been successfully and individually investigated in these laboratory-on-a-chip systems. Also, 5 micron polystyrene particles have been separated from the aqueous microparticle suspensions in the microfluidic lab-on-a-chip systems of modified design with 98% separation efficiency, and 10 micron polystyrene particles have been separated with 100% separation efficiency. About the novelty of this work, the experimental investigations have been performed on the surface-driven microfluidic flow of aqueous microparticle suspensions with the investigations on the separation time in particle-size based separation mechanism to control these suspensions in the microfluidic lab-on-a-chip systems. This research work contains a total of 10,112 individual experimental outputs obtained using total 30 individual instruments by author’s own hands-on completely during more than three years continuously. Author has performed the experimental investigations on both the fluid statics and fluid dynamics to develop an automated fluid machine.

Contribution to the theory of stationary separation areas

NASA Technical Reports Server (NTRS)

Taganov, G. I.

1985-01-01

An attempt is made to determine the region of existence of possible steady flows with a closed separation area in a range of Reynolds numbers such that flow in the viscous mixing area can be described by the Prandtl's equations. The boundary conditions for the flow in the separation region are selected so as to simplify the flow pattern in this region, making it possible to use the methods of hydrodynamic analysis. A rule for determining stable steady flows with separation areas is formulated which is well suited for analyzing laminar flows and can be applied to turbulent flows in some areas.

Viscous investigation of a flapping foil propulsor

NASA Astrophysics Data System (ADS)

Posri, Attapol; Phoemsapthawee, Surasak; Thaweewat, Nonthipat

2018-01-01

Inspired by how fishes propel themselves, a flapping-foil device is invented as an alternative propulsion system for ships and boats. The performance of such propulsor has been formerly investigated using a potential flow code. The simulation results have shown that the device has high propulsive efficiency over a wide range of operation. However, the potential flow gives good results only when flow separation is not present. In case of high flapping frequency, the flow separation can occur over a short instant due to fluid viscosity and high angle of attack. This may cause a reduction of propulsive efficiency. A commercial CFD code based on Lattice Boltzmann Method, XFlow, is then employed in order to investigate the viscous effect over the propulsive performance of the flapping foil. The viscous results agree well with the potential flow results, confirming the high efficiency of the propulsor. As expected, viscous results show lower efficiency in high flapping frequency zone.

Control of flow separation in a turbulent boundary layer

NASA Astrophysics Data System (ADS)

Cho, Minjeong; Choi, Sangho; Choi, Haecheon

2015-11-01

Towards the development of successful control methods for separation delay in a turbulent boundary layer, we adopt a model flow field, in which a turbulent separation occurs above a flat plate (Na and Moin 1998 JFM), and apply controls to this flow for reducing the size of the separation bubble and investigating the interaction between the forcing and flow near the separation bubble. We provide a single-frequency forcing with zero net mass flow rate at the upstream of the separation bubble. At low forcing frequencies, spanwise vortices are generated and travel downstream, bringing high momentum toward the wall and reducing the size of the separation bubble. Also, these vortices cause the separation and reattachment points to travel downstream. On the other hand, at high forcing frequencies, the size of the separation bubble becomes smaller and larger in time, respectively, due to the pressure gradient alternating favorably and adversely in time. Supported by NRF-2011-0028032 and 2014048162.

Separate Flow Nozzle Test Status Meeting

NASA Technical Reports Server (NTRS)

Saiyed, Naseem H. (Editor)

2000-01-01

NASA Glenn, in partnership with US industry, completed an exhaustive experimental study on jet noise reduction from separate flow nozzle exhaust systems. The study developed a data base on various bypass ratio nozzles, screened quietest configurations and acquired pertinent data for predicting the plume behavior and ultimately its corresponding jet noise. Several exhaust system configurations provided over 2.5 EPNdB jet noise reduction at take-off power. These data were disseminated to US aerospace industry in a conference hosted by NASA GRC whose proceedings are shown in this report.

Hormone purification by isoelectric focusing in space

NASA Technical Reports Server (NTRS)

Bier, M.

1988-01-01

The objective of the program was the definition and development of optimal methods for electrophoretic separations in microgravity. The approach is based on a triad consisting of ground based experiments, mathematical modeling and experiments in microgravity. Zone electrophoresis is a rate process, where separation is achieved in uniform buffers on the basis of differences in electrophoretic mobilities. Optimization and modeling of continuous flow electrophoresis mainly concern the hydrodynamics of the flow process, including gravity dependent fluid convection due to density gradients and gravity independent electroosmosis. Optimization of focusing requires a more complex model describing the molecular transport processes involved in electrophoresis of interacting systems. Three different focusing instruments were designed, embodying novel principles of fluid stabilization. Fluid stability was achieved by: (1) flow streamlining by means of membrane elements in combination with rapid fluid recycling; (2) apparatus rotation in combination with said membrane elements; and (3) shear stress induced by rapid recycling through a narrow gap channel.

Parametric Study of Synthetic-Jet-Based Flow Control on a Vertical Tail Model

NASA Astrophysics Data System (ADS)

Monastero, Marianne; Lindstrom, Annika; Beyar, Michael; Amitay, Michael

2015-11-01

Separation control over the rudder of the vertical tail of a commercial airplane using synthetic-jet-based flow control can lead to a reduction in tail size, with an associated decrease in drag and increase in fuel savings. A parametric, experimental study was undertaken using an array of finite span synthetic jets to investigate the sensitivity of the enhanced vertical tail side force to jet parameters, such as jet spanwise spacing and jet momentum coefficient. A generic wind tunnel model was designed and fabricated to fundamentally study the effects of the jet parameters at varying rudder deflection and model sideslip angles. Wind tunnel results obtained from pressure measurements and tuft flow visualization in the Rensselaer Polytechnic Subsonic Wind Tunnel show a decrease in separation severity and increase in model performance in comparison to the baseline, non-actuated case. The sensitivity to various parameters will be presented.

Cluster-based control of a separating flow over a smoothly contoured ramp

NASA Astrophysics Data System (ADS)

Kaiser, Eurika; Noack, Bernd R.; Spohn, Andreas; Cattafesta, Louis N.; Morzyński, Marek

2017-12-01

The ability to manipulate and control fluid flows is of great importance in many scientific and engineering applications. The proposed closed-loop control framework addresses a key issue of model-based control: The actuation effect often results from slow dynamics of strongly nonlinear interactions which the flow reveals at timescales much longer than the prediction horizon of any model. Hence, we employ a probabilistic approach based on a cluster-based discretization of the Liouville equation for the evolution of the probability distribution. The proposed methodology frames high-dimensional, nonlinear dynamics into low-dimensional, probabilistic, linear dynamics which considerably simplifies the optimal control problem while preserving nonlinear actuation mechanisms. The data-driven approach builds upon a state space discretization using a clustering algorithm which groups kinematically similar flow states into a low number of clusters. The temporal evolution of the probability distribution on this set of clusters is then described by a control-dependent Markov model. This Markov model can be used as predictor for the ergodic probability distribution for a particular control law. This probability distribution approximates the long-term behavior of the original system on which basis the optimal control law is determined. We examine how the approach can be used to improve the open-loop actuation in a separating flow dominated by Kelvin-Helmholtz shedding. For this purpose, the feature space, in which the model is learned, and the admissible control inputs are tailored to strongly oscillatory flows.

Passive Flap Actuation by Reversing Flow in Laminar Boundary Layer Separation

NASA Astrophysics Data System (ADS)

Parsons, Chase; Lang, Amy; Santos, Leo; Bonacci, Andrew

2017-11-01

Reducing the flow separation is of great interest in the field of fluid mechanics in order to reduce drag and improve the overall efficiency of aircraft. This project seeks to investigate passive flow control using shark inspired microflaps in laminar boundary layer separation. This study aims to show that whether a flow is laminar or turbulent, laminar and 2D or turbulent and 3D, microflaps actuated by reversing flow is a robust means of controlling flow separation. In order to generate a controlled adverse pressure gradient, a rotating cylinder induces separation at a chosen location on a flat plate boundary layer with Re above 10000. Within this thick boundary layer, digital particle image velocimetry is used to map the flow. This research can be used in the future to better understand the nature of the bristling shark scales and its ability to passively control separation. Results show that microflaps successfully actuated due to backflow and that this altered the formation of flow separation. I would like to thank the NSF for REU Grant EEC 1659710 and the Army Research Office for funding this project.

Computed Flow Through An Artificial Heart Valve

NASA Technical Reports Server (NTRS)

Rogers, Stewart E.; Kwak, Dochan; Kiris, Cetin; Chang, I-Dee

1994-01-01

Report discusses computations of blood flow through prosthetic tilting disk valve. Computational procedure developed in simulation used to design better artificial hearts and valves by reducing or eliminating following adverse flow characteristics: large pressure losses, which prevent hearts from working efficiently; separated and secondary flows, which causes clotting; and high turbulent shear stresses, which damages red blood cells. Report reiterates and expands upon part of NASA technical memorandum "Computed Flow Through an Artificial Heart and Valve" (ARC-12983). Also based partly on research described in "Numerical Simulation of Flow Through an Artificial Heart" (ARC-12478).

A review of exosome separation techniques and characterization of B16-F10 mouse melanoma exosomes with AF4-UV-MALS-DLS-TEM.

PubMed

Petersen, Kevin E; Manangon, Eliana; Hood, Joshua L; Wickline, Samuel A; Fernandez, Diego P; Johnson, William P; Gale, Bruce K

2014-12-01

Exosomes participate in cancer metastasis, but studying them presents unique challenges as a result of their small size and purification difficulties. Asymmetrical field flow fractionation with in-line ultraviolet absorbance, dynamic light scattering, and multi-angle light scattering was applied to the size separation and characterization of non-labeled B16-F10 exosomes from an aggressive mouse melanoma cell culture line. Fractions were collected and further analyzed using batch mode dynamic light scattering, transmission electron microscopy and compared with known size standards. Fractogram peak positions and computed radii show good agreement between samples and across fractions. Ultraviolet absorbance fractograms in combination with transmission electron micrographs were able to resolve subtle heterogeneity of vesicle retention times between separate batches of B16-F10 exosomes collected several weeks apart. Further, asymmetrical field flow fractionation also effectively separated B16-F10 exosomes into vesicle subpopulations by size. Overall, the flow field flow fractionation instrument combined with multiple detectors was able to rapidly characterize and separate exosomes to a degree not previously demonstrated. These approaches have the potential to facilitate a greater understanding of exosome function by subtype, as well as ultimately allow for "label-free" isolation of large scale clinical exosomes for the purpose of developing future exosome-based diagnostics and therapeutics.

A review of exosome separation techniques and characterization of B16-F10 mouse melanoma exosomes with AF4-UV-MALS-DLS-TEM

PubMed Central

Manangon, Eliana; Hood, Joshua L.; Wickline, Samuel A.; Fernandez, Diego P.; Johnson, William P.; Gale, Bruce K.

2015-01-01

Exosomes participate in cancer metastasis, but studying them presents unique challenges as a result of their small size and purification difficulties. Asymmetrical field flow fractionation with in-line ultraviolet absorbance, dynamic light scattering, and multi-angle light scattering was applied to the size separation and characterization of non-labeled B16-F10 exosomes from an aggressive mouse melanoma cell culture line. Fractions were collected and further analyzed using batch mode dynamic light scattering, transmission electron microscopy and compared with known size standards. Fractogram peak positions and computed radii show good agreement between samples and across fractions. Ultraviolet absorbance fractograms in combination with transmission electron micrographs were able to resolve subtle heterogeneity of vesicle retention times between separate batches of B16-F10 exosomes collected several weeks apart. Further, asymmetrical field flow fractionation also effectively separated B16-F10 exosomes into vesicle subpopulations by size. Overall, the flow field flow fractionation instrument combined with multiple detectors was able to rapidly characterize and separate exosomes to a degree not previously demonstrated. These approaches have the potential to facilitate a greater understanding of exosome function by subtype, as well as ultimately allow for “label-free” isolation of large scale clinical exosomes for the purpose of developing future exosome-based diagnostics and therapeutics. PMID:25084738

PFB coal fired combined cycle development program. Advanced hot gas cleanup concept evaluation (Task 4. 3). Volume A. Aerodyne cyclone evaluation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This report summarizes the results of testing of a rotary flow cyclone, manufactured by Aerodyne Development Corporation under license by Siemens Kraftwerk Union. This cyclone was selected for evaluation due to the unusually high separative efficiencies claimed by the manufacturer (based on developer data), and relative lack of open literature data. The most significant finding of this work was the observation that electrostatic forces could enhance or, in fact, dominate the separation process. Separative efficiencies, with electrostatic forces present, were found to be substantially independent of flow rate and, by inference, could be independent of unit size. This finding suggestsmore » that large cyclones with natural or augmented electrostatic forces employed in the hot gas cleanup train of the CFCC system may not suffer the performance degradation compared to small cyclones, as projected from conventional inertial theory. This is of special importance since the use of many small cyclones in parallel, or multicyclones, commonly suffers from fouling and this approach is not recommended in the CFCC application. The original objective of this investigation was to assess the relative merits of the Aerodyne cyclone separator. It was found from both the cold flow and the hot flow tests that its separative efficiencies are disappointingly poorer than expectations (in agreement with Westinghouse results), and even poorer than conventional cyclones.« less

Some observations of separated flow on finite wings

NASA Technical Reports Server (NTRS)

Winkelmann, A. E.; Ngo, H. T.; De Seife, R. C.

1982-01-01

Wind tunnel test results for aspects of flow over airfoils exhibiting single and multiple trailing edge stall 'mushroom' cells are reported. Rectangular wings with aspect ratios of 4.0 and 9.0 were tested at Reynolds numbers of 480,000 and 257,000, respectively. Surface flow patterns were visualized by means of a fluorescent oil flow technique, separated flow was observed with a tuft wand and a water probe, spanwise flow was studied with hot-wire anemometry, smoke flow and an Ar laser illuminated the centerplane flow, and photographs were made of the oil flow patterns. Swirl patterns on partially and fully stalled wings suggested vortex flow attachments in those regions, and a saddle point on the fully stalled AR=4.0 wing indicated a secondary vortex flow at the forward region of the separation bubble. The separation wake decayed downstream, while the tip vortex interacted with the separation bubble on the fully stalled wing. Three mushroom cells were observed on the AR=9.0 wing.

Feasibility study of red blood cell debulking by magnetic field-flow fractionation with step-programmed flow

PubMed Central

Moore, Lee R.; Williams, P. Stephen; Nehl, Franziska; Abe, Koji; Chalmers, Jeffrey J.; Zborowski, Maciej

2013-01-01

Emerging applications of rare cell separation and analysis, such as separation of mature red blood cells from hematopoietic cell cultures require efficient methods of red blood cell (RBC) debulking. We have tested the feasibility of magnetic RBC separation as an alternative to centrifugal separation using an approach based on the mechanism of magnetic field-flow fractionation (MgFFF). A specially designed permanent magnet assembly generated a quadrupole field having a maximum field of 1.68 T at the magnet pole tips, zero field at the aperture axis, and a nearly constant radial field gradient of 1.75 T/mm (with a negligible angular component) inside a cylindrical aperture of 1.9 mm (diameter) and 76 mm (length). The cell samples included high-spin hemoglobin RBCs obtained by chemical conversion of hemoglobin to methemoglobin (met RBC) or by exposure to anoxic conditions (deoxy RBC), low-spin hemoglobin obtained by exposure of RBC suspension to ambient air (oxy RBC), and mixtures of deoxy RBC and cells from a KG-1a white blood cell (WBC) line. The observation that met RBCs did not elute from the channel at the lower flow rate of 0.05 mL/min applied for 15 min but quickly eluted at the subsequent higher flow rate of 2.0 mL/min was in agreement with FFF theory. The well-defined experimental conditions (precise field and flow characteristics) and a well-established FFF theory verified by studies with model cell systems provided us with a strong basis for making predictions about potential practical applications of the magnetic RBC separation. PMID:24141316

Feasibility study of red blood cell debulking by magnetic field-flow fractionation with step-programmed flow.

PubMed

Moore, Lee R; Williams, P Stephen; Nehl, Franziska; Abe, Koji; Chalmers, Jeffrey J; Zborowski, Maciej

2014-02-01

Emerging applications of rare cell separation and analysis, such as separation of mature red blood cells from hematopoietic cell cultures, require efficient methods of red blood cell (RBC) debulking. We have tested the feasibility of magnetic RBC separation as an alternative to centrifugal separation using an approach based on the mechanism of magnetic field-flow fractionation (MgFFF). A specially designed permanent magnet assembly generated a quadrupole field having a maximum field of 1.68 T at the magnet pole tips, zero field at the aperture axis, and a nearly constant radial field gradient of 1.75 T/mm (with a negligible angular component) inside a cylindrical aperture of 1.9 mm (diameter) and 76 mm (length). The cell samples included high-spin hemoglobin RBCs obtained by chemical conversion of hemoglobin to methemoglobin (met RBC) or by exposure to anoxic conditions (deoxy RBC), low-spin hemoglobin obtained by exposure of RBC suspension to ambient air (oxy RBC), and mixtures of deoxy RBC and cells from a KG-1a white blood cell (WBC) line. The observation that met RBCs did not elute from the channel at the lower flow rate of 0.05 mL/min applied for 15 min but quickly eluted at the subsequent higher flow rate of 2.0 mL/min was in agreement with FFF theory. The well-defined experimental conditions (precise field and flow characteristics) and a well-established FFF theory verified by studies with model cell systems provided us with a strong basis for making predictions about potential practical applications of the magnetic RBC separation.

Separation Dynamics of Controlled Internal Flow in an Adverse Pressure Gradient

NASA Astrophysics Data System (ADS)

Peterson, C. J.; Vukasinovic, B.; Glezer, A.

2017-11-01

The effects of fluidic actuation on the dynamic evolution of aggressive internal flow separation is investigated at speeds up to M = 0.4 within a constant-width diffuser branching off of a primary flow duct. It is shown that a spanwise array of fluidic actuators upstream of the separation actively controls the flow constriction (and losses) within the diffuser and consequently the local pressure gradient at its entrance. The effectiveness of the actuation, as may be measured by the increased flow rate that is diverted through the diffuser, scales with its flow rate coefficient. In the presence of actuation (0.7% mass fraction), the mass flow rate in the primary duct increases by 10% while the fraction of the diverted mass flow rate in the diffuser increases by more than 45%. The flow dynamics near separation in the absence and presence of actuation are characterized using high speed particle image velocimetry and analyzed using proper orthogonal and spectral decompositions. In particular, the spectral contents of the incipient boundary layer separation are compared in the absence and presence of actuation with emphasis on the changes in local dynamics near separation as the characteristic cross stream scale of the boundary layer increases with separation delay.

CO2-vegetation feedbacks and other climate changes implicated in reducing base flow

NASA Astrophysics Data System (ADS)

Trancoso, Ralph; Larsen, Joshua R.; McVicar, Tim R.; Phinn, Stuart R.; McAlpine, Clive A.

2017-03-01

Changes in the hydrological cycle have a significant impact in water limited environments. Globally, some of these regions are experiencing declining precipitation yet are simultaneously becoming greener, partly due to vegetation feedbacks associated with increasing atmospheric CO2 concentrations. Reduced precipitation together with increasing rates of actual evapotranspiration diminishes streamflow, especially base flow, a critical freshwater dry-season resource. Here we assess recent changes in base flow in Australia from 1981-2013 and 1950-2013 and separate the contribution of precipitation, potential evapotranspiration, and other factors on base flow trends. Our findings reveal that these other factors influencing the base flow trends are best explained by an increase in photosynthetic activity. These results provide the first robust observational evidence that increasing atmospheric CO2 and its associated vegetation feedbacks are reducing base flow in addition to other climatic impacts. These findings have broad implications for water resource management, especially in the world's water limited regions.

Active Flow Effectors for Noise and Separation Control

NASA Technical Reports Server (NTRS)

Turner, Travis L.

2011-01-01

New flow effector technology for separation control and enhanced mixing is based upon shape memory alloy hybrid composite (SMAHC) technology. The technology allows for variable shape control of aircraft structures through actively deformable surfaces. The flow effectors are made by embedding shape memory alloy actuator material in a composite structure. When thermally actuated, the flow effector def1ects into or out of the flow in a prescribed manner to enhance mixing or induce separation for a variety of applications, including aeroacoustic noise reduction, drag reduction, and f1ight control. The active flow effectors were developed for noise reduction as an alternative to fixed-configuration effectors, such as static chevrons, that cannot be optimized for airframe installation effects or variable operating conditions and cannot be retracted for off-design or fail-safe conditions. Benefits include: Increased vehicle control, overall efficiency, and reduced noise throughout all f1ight regimes, Reduced flow noise, Reduced drag, Simplicity of design and fabrication, Simplicity of control through direct current stimulation, autonomous re sponse to environmental heating, fast re sponse, and a high degree of geometric stability. The concept involves embedding prestrained SMA actuators on one side of the chevron neutral axis in order to generate a thermal moment and def1ect the structure out of plane when heated. The force developed in the host structure during def1ection and the aerodynamic load is used for returning the structure to the retracted position. The chevron design is highly scalable and versatile, and easily affords active and/or autonomous (environmental) control. The technology offers wide-ranging market applications, including aerospace, automotive, and any application that requires flow separation or noise control.

Improved prediction of disturbed flow via hemodynamically-inspired geometric variables.

PubMed

Bijari, Payam B; Antiga, Luca; Gallo, Diego; Wasserman, Bruce A; Steinman, David A

2012-06-01

Arterial geometry has long been considered as a pragmatic alternative for inferring arterial flow disturbances, and their impact on the natural history and treatment of vascular diseases. Traditionally, definition of geometric variables is based on convenient shape descriptors, with only superficial consideration of their influence on flow and wall shear stress patterns. In the present study we demonstrate that a more studied consideration of the actual (cf. nominal) local hemodynamics can lead to substantial improvements in the prediction of disturbed flow by geometry. Starting from a well-characterized computational fluid dynamics (CFD) dataset of 50 normal carotid bifurcations, we observed that disturbed flow tended to be confined proximal to the flow divider, whereas geometric variables previously shown to be significant predictors of disturbed flow included features distal to the flow divider in their definitions. Flaring of the bifurcation leading to flow separation was redefined as the maximum relative expansion of the common carotid artery (CCA), proximal to the flow divider. The beneficial effect of primary curvature on flow inertia, via suppression of flow separation, was characterized by the in-plane tortuosity of CCA as it enters the flare region. Multiple linear regressions of these redefined geometric variables against various metrics of disturbed flow revealed R(2) values approaching 0.6, better than the roughly 0.3 achieved using the conventional shape-based variables, while maintaining their demonstrated real-world reproducibility. Such a hemodynamically-inspired approach to the definition of geometric variables may reap benefits for other applications where geometry is used as a surrogate marker of local hemodynamics. Copyright © 2012 Elsevier Ltd. All rights reserved.

Antibody-immobilized column for quick cell separation based on cell rolling.

PubMed

Mahara, Atsushi; Yamaoka, Tetsuji

2010-01-01

Cell separation using methodological standards that ensure high purity is a very important step in cell transplantation for regenerative medicine and for stem cell research. A separation protocol using magnetic beads has been widely used for cell separation to isolate negative and positive cells. However, not only the surface marker pattern, e.g., negative or positive, but also the density of a cell depends on its developmental stage and differentiation ability. Rapid and label-free separation procedures based on surface marker density are the focus of our interest. In this study, we have successfully developed an antiCD34 antibody-immobilized cell-rolling column, that can separate cells depending on the CD34 density of the cell surfaces. Various conditions for the cell-rolling column were optimized including graft copolymerization, and adjustment of the column tilt angle, and medium flow rate. Using CD34-positive and -negative cell lines, the cell separation potential of the column was established. We observed a difference in the rolling velocities between CD34-positive and CD34-negative cells on antibody-immobilized microfluidic device. Cell separation was achieved by tilting the surface 20 degrees and the increasing medium flow. Surface marker characteristics of the isolated cells in each fraction were analyzed using a cell-sorting system, and it was found that populations containing high density of CD34 were eluted in the delayed fractions. These results demonstrate that cells with a given surface marker density can be continuously separated using the cell rolling column.

Tapered Microfluidic for Continuous Micro-Object Separation Based on Hydrodynamic Principle.

PubMed

Ahmad, Ida Laila; Ahmad, Mohd Ridzuan; Takeuchi, Masaru; Nakajima, Masahiro; Hasegawa, Yasuhisa

2017-12-01

Recent advances in microfluidic technologies have created a demand for a simple and efficient separation intended for various applications such as food industries, biological preparation, and medical diagnostic. In this paper, we report a tapered microfluidic device for passive continuous separation of microparticles by using hydrodynamic separation. By exploiting the hydrodynamic properties of the fluid flow and physical characteristics of micro particles, effective size based separation is demonstrated. The tapered microfluidic device has widening geometries with respect to specific taper angle which amplify the sedimentation effect experienced by particles of different sizes. A mixture of 3-μm and 10-μm polystyrene microbeads are successfully separated using 20° and 25° taper angles. The results obtained are in agreement with three-dimensional finite element simulation conducted using Abaqus 6.12. Moreover, the feasibility of this mechanism for biological separation is demonstrated by using polydisperse samples consists of 3-μm polystyrene microbeads and human epithelial cervical carcinoma (HeLa) cells. 98% of samples purity is recovered at outlet 1 and outlet 3 with flow rate of 0.5-3.0 μl/min. Our device is interesting despite adopting passive separation approach. This method enables straightforward, label-free, and continuous separation of multiparticles in a stand-alone device without the need for bulky apparatus. Therefore, this device may become an enabling technology for point of care diagnosis tools and may hold potential for micrototal analysis system applications.

Quality testing of an innovative cascade separation system for multiple cell separation

NASA Astrophysics Data System (ADS)

Pierzchalski, Arkadiusz; Moszczynska, Aleksandra; Albrecht, Bernd; Heinrich, Jan-Michael; Tarnok, Attila

2012-03-01

Isolation of different cell types from mixed samples in one separation step by FACS is feasible but expensive and slow. It is cheaper and faster but still challenging by magnetic separation. An innovative bead-based cascade-system (pluriSelect GmbH, Leipzig, Germany) relies on simultaneous physical separation of different cell types. It is based on antibody-mediated binding of cells to beads of different size and isolation with sieves of different mesh-size. We validated pluriSelect system for single parameter (CD3) and simultaneous separation of CD3 and CD15 cells from EDTA blood-samples. Results were compared with those obtained by MACS (Miltenyi-Biotech) magnetic separation (CD3 separation). pluriSelect separation was done in whole blood, MACS on Ficoll gradient isolated leukocytes, according to the manufacturer's protocols. Isolated and residual cells were immunophenotyped (7-color 8-antibody panel (CD3; CD16/56; CD4; CD8; CD14; CD19; CD45; HLADR) on a CyFlowML flow cytometer (Partec GmbH). Cell count (Coulter), purity, yield and viability (7-AAD exclusion) were determined. There were no significant differences between both systems regarding purity (92-98%), yield (50-60%) and viability (92-98%) of isolated cells. PluriSelect separation was slightly faster than MACS (1.15 h versus 1.5h). Moreover, no preenrichment steps were necessary. In conclusion, pluriSelect is a fast, simple and gentle system for efficient simultaneous separation of two cell subpopulation directly from whole blood and can provide a simple alternative to FACS. The isolated cells can be used for further research applications.

Hydrodynamic interaction of two deformable drops in confined shear flow.

PubMed

Chen, Yongping; Wang, Chengyao

2014-09-01

We investigate hydrodynamic interaction between two neutrally buoyant circular drops in a confined shear flow based on a computational fluid dynamics simulation using the volume-of-fluid method. The rheological behaviors of interactive drops and the flow regimes are explored with a focus on elucidation of underlying physical mechanisms. We find that two types of drop behaviors during interaction occur, including passing-over motion and reversing motion, which are governed by the competition between the drag of passing flow and the entrainment of reversing flow in matrix fluid. With the increasing confinement, the drop behavior transits from the passing-over motion to reversing motion, because the entrainment of the reversing-flow matrix fluid turns to play the dominant role. The drag of the ambient passing flow is increased by enlarging the initial lateral separation due to the departure of the drop from the reversing flow in matrix fluid, resulting in the emergence of passing-over motion. In particular, a corresponding phase diagram is plotted to quantitatively illustrate the dependence of drop morphologies during interaction on confinement and initial lateral separation.

A Structured Grid Based Solution-Adaptive Technique for Complex Separated Flows

NASA Technical Reports Server (NTRS)

Thornburg, Hugh; Soni, Bharat K.; Kishore, Boyalakuntla; Yu, Robert

1996-01-01

The objective of this work was to enhance the predictive capability of widely used computational fluid dynamic (CFD) codes through the use of solution adaptive gridding. Most problems of engineering interest involve multi-block grids and widely disparate length scales. Hence, it is desirable that the adaptive grid feature detection algorithm be developed to recognize flow structures of different type as well as differing intensity, and adequately address scaling and normalization across blocks. In order to study the accuracy and efficiency improvements due to the grid adaptation, it is necessary to quantify grid size and distribution requirements as well as computational times of non-adapted solutions. Flow fields about launch vehicles of practical interest often involve supersonic freestream conditions at angle of attack exhibiting large scale separate vortical flow, vortex-vortex and vortex-surface interactions, separated shear layers and multiple shocks of different intensity. In this work, a weight function and an associated mesh redistribution procedure is presented which detects and resolves these features without user intervention. Particular emphasis has been placed upon accurate resolution of expansion regions and boundary layers. Flow past a wedge at Mach=2.0 is used to illustrate the enhanced detection capabilities of this newly developed weight function.

Numerical simulation of separated flows. Ph.D. Thesis - Stanford Univ., Calif.

NASA Technical Reports Server (NTRS)

Spalart, P. R.; Leonard, A.; Baganoff, D.

1983-01-01

A new numerical method, based on the Vortex Method, for the simulation of two-dimensional separated flows, was developed and tested on a wide range of gases. The fluid is incompressible and the Reynolds number is high. A rigorous analytical basis for the representation of the Navier-Stokes equation in terms of the vorticity is used. An equation for the control of circulation around each body is included. An inviscid outer flow (computed by the Vortex Method) was coupled with a viscous boundary layer flow (computed by an Eulerian method). This version of the Vortex Method treats bodies of arbitrary shape, and accurately computes the pressure and shear stress at the solid boundary. These two quantities reflect the structure of the boundary layer. Several versions of the method are presented and applied to various problems, most of which have massive separation. Comparison of its results with other results, generally experimental, demonstrates the reliability and the general accuracy of the new method, with little dependence on empirical parameters. Many of the complex features of the flow past a circular cylinder, over a wide range of Reynolds numbers, are correctly reproduced.

Separation control in a hypersonic shock wave / turbulent boundary-layer interaction

NASA Astrophysics Data System (ADS)

Schreyer, Anne-Marie; Bermejo-Moreno, Ivan; Kim, Jeonglae; Urzay, Javier

2016-11-01

Hypersonic vehicles play a key role for affordable access to space. The associated flow fields are strongly affected by shock wave/turbulent boundary-layer interactions, and the inherent separation causes flow distortion and low-frequency unsteadiness. Microramp sub-boundary layer vortex generators are a promising means to control separation and diminish associated detrimental effects. We investigate the effect of a microramp on the low-frequency unsteadiness in a fully separated interaction. A large eddy simulation of a 33 ∘ -compression-ramp interaction was performed for an inflow Mach number of 7.2 and a Reynolds number based on momentum thickness of Reθ = 3500 , matching the experiment of Schreyer et al. (2011). For the control case, we introduced a counter-rotating vortex pair, as induced by a single microramp, into the boundary layer through the inflow conditions. We applied a dynamic mode decomposition (DMD) on both cases to identify coherent structures that are responsible for the dynamic behavior. Based on the DMD, we discuss the reduction of the separation zone and the stabilization of the shock motion achieved by the microramp, and contribute to the description of the governing mechanisms. Pursued during the 2016 CTR Summer Program at Stanford University.

Glow Discharge Plasma Demonstrated for Separation Control in the Low-Pressure Turbine

NASA Technical Reports Server (NTRS)

Ashpis, David e.; Hultgren, Lennart S.

2004-01-01

Flow separation in the low-pressure turbine (LPT) is a major barrier that limits further improvements of aerodynamic designs of turbine airfoils. The separation is responsible for performance degradation, and it prevents the design of highly loaded airfoils. The separation can be delayed, reduced, or eliminated completely if flow control techniques are used. Successful flow control technology will enable breakthrough improvements in gas turbine performance and design. The focus of this research project was the development and experimental demonstration of active separation control using glow discharge plasma (GDP) actuators in flow conditions simulating the LPT. The separation delay was shown to be successful, laying the foundation for further development of the technologies to practical application in the LPT. In a fluid mechanics context, the term "flow control" means a technology by which a very small input results in a very large effect on the flow. In this project, the interest is to eliminate or delay flow separation on LPT airfoils by using an active flow control approach, in which disturbances are dynamically inserted into the flow, they interact with the flow, and they delay separation. The disturbances can be inserted using a localized, externally powered, actuating device, examples are acoustic, pneumatic, or mechanical devices that generate vibrations, flow oscillations, or pulses. A variety of flow control devices have been demonstrated in recent years in the context of the external aerodynamics of aircraft wings and airframes, where the incoming flow is quiescent or of a very low turbulence level. However, the flow conditions in the LPT are significantly different because there are high levels of disturbances in the incoming flow that are characterized by high free-stream turbulence intensity. In addition, the Reynolds number, which characterizes the viscous forces in the flow and is related to the flow speed, is very low in the LPT passages.

A Resonant Pulse Detonation Actuator for High-Speed Boundary Layer Separation Control

NASA Technical Reports Server (NTRS)

Beck, B. T.; Cutler, A. D.; Drummond, J. P.; Jones, S. B.

2004-01-01

A variety of different types of actuators have been previously investigated as flow control devices. Potential applications include the control of boundary layer separation in external flows, as well as jet engine inlet and diffuser flow control. The operating principles for such devices are typically based on either mechanical deflection of control surfaces (which include MEMS flap devices), mass injection (which includes combustion driven jet actuators), or through the use of synthetic jets (diaphragm devices which produce a pulsating jet with no net mass flow). This paper introduces some of the initial flow visualization work related to the development of a relatively new type of combustion-driven jet actuator that has been proposed based on a pulse detonation principle. The device is designed to utilize localized detonation of a premixed fuel (Hydrogen)-air mixture to periodically inject a jet of gas transversely into the primary flow. Initial testing with airflow successfully demonstrated resonant conditions within the range of acoustic frequencies expected for the design. Schlieren visualization of the pulsating air jet structure revealed axially symmetric vortex flow, along with the formation of shocks. Flow visualization of the first successful sustained oscillation condition is also demonstrated for one configuration of the current test section. Future testing will explore in more detail the onset of resonant combustion and the approach to conditions of sustained resonant detonation.

Bluff-body drag reduction using a deflector

NASA Astrophysics Data System (ADS)

Fourrié, Grégoire; Keirsbulck, Laurent; Labraga, Larbi; Gilliéron, Patrick

2011-02-01

A passive flow control on a generic car model was experimentally studied. This control consists of a deflector placed on the upper edge of the model rear window. The study was carried out in a wind tunnel at Reynolds numbers based on the model height of 3.1 × 105 and 7.7 × 105. The flow was investigated via standard and stereoscopic particle image velocimetry, Kiel pressure probes and surface flow visualization. The aerodynamic drag was measured using an external balance and calculated using a wake survey method. Drag reductions up to 9% were obtained depending on the deflector angle. The deflector increases the separated region on the rear window. The results show that when this separated region is wide enough, it disrupts the development of the counter-rotating longitudinal vortices appearing on the lateral edges of the rear window. The current study suggests that flow control on such geometries should consider all the flow structures that contribute to the model wake flow.

Flow processes in electric discharge drivers

NASA Technical Reports Server (NTRS)

Baganoff, D.

1975-01-01

The performance of an electric discharge shock tube is discussed from the point of view that the conditions at the sonic station are the primary controlling variables (likewise in comparing designs), and that the analysis of the flow on either side of the sonic station should be done separately. The importance of considering mass-flow rate in matching a given driver design to the downstream flow required for a particular shock-wave speed is stressed. It is shown that a driver based on the principle of liquid injection (of H2) is superior to one based on the Ludwieg tube, because of the greater mass-flow rate and the absence of a massive diaphragm.

Impact of non-ideal analyte behavior on the separation of protein aggregates by asymmetric flow field-flow fractionation.

PubMed

Boll, Björn; Josse, Lena; Heubach, Anja; Hochenauer, Sophie; Finkler, Christof; Huwyler, Jörg; Koulov, Atanas V

2018-04-25

Asymmetric flow field-flow fractionation is a valuable tool for the characterization of protein aggregates in biotechnology owing to its broad size range and unique separation principle. However, in practice asymmetric flow field-flow fractionation is non-trivial to use due to the major deviations from theory and the influence on separation by various factors that are not fully understood. Here we report methods to assess the non-ideal effects that influence asymmetric flow field-flow fractionation separation and for the first time identify experimentally the main factors that impact it. Furthermore, we propose new approaches to minimize such non-ideal behavior, showing that by adjusting the mobile phase composition (pH and ionic strength) the resolution of asymmetric flow field-flow fractionation separation can be drastically improved. Additionally, we propose a best practice method for new proteins. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Cross-flow-assembled ultrathin and robust graphene oxide membranes for efficient molecule separation

NASA Astrophysics Data System (ADS)

Ying, Yulong; Ying, Wen; Guo, Yi; Peng, Xinsheng

2018-04-01

A graphene oxide (GO) membrane is promising for molecule separation. However, it is still a big challenge to achieve highly stable pristine GO membranes, especially in water. In this work, an ultrathin and robust GO membrane is assembled via the cross-flow method. The as-prepared 12 nm thick GO membrane (GOCF membrane) presents high stability with water permeance of 1505 ± 65 litres per hour per square meter per bar (LHM bar-1) and Evans Blue (EB) rejection of 98.7 ± 0.4%, 21-fold enhancement in water permeance compared with that of a pristine GO membrane (50-70 LHM bar-1) and 100 times higher than that of commercial ultrafiltration membranes (15 LHM.bar-1, GE2540F30, MWCO 1000, GE Co., Ltd) with similar rejection. Attributed to the surface cross-flow, the GO nanosheets will be refolded, crumpled, or wrinkled, resulting in a very strong inter-locking structure among the GO membrane, which significantly enhances the stability and facilitates their separation performance. This cross-flow assembling technique is also easily extended to assemble GO membranes onto other various backing filter supports. Based on the Donnan effect and size sieving mechanism, selective membrane separation of dyes with a similar molecular structure from their mixture (such as Rhodamine B (RhB) and Rose Bengal, and RhB and EB) are achieved with a selectivity of 133 ± 10 and 227 ± 15, respectively. Assembly of this ultrathin GO membrane with high stability and separation performance, via a simple cross-flow method, shows great potential for water purification.

Use of exhaust gas as sweep flow to enhance air separation membrane performance

DOEpatents

Dutart, Charles H.; Choi, Cathy Y.

2003-01-01

An intake air separation system for an internal combustion engine is provided with purge gas or sweep flow on the permeate side of separation membranes in the air separation device. Exhaust gas from the engine is used as a purge gas flow, to increase oxygen flux in the separation device without increasing the nitrogen flux.

Separation of CHO cells using hydrocyclones.

PubMed

Pinto, Rodrigo C V; Medronho, Ricardo A; Castilho, Leda R

2008-01-01

Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design. It was shown that cell separation efficiencies for cultures of the wild-type CHO.K1 cell line and of a recombinant CHO cell line producing granulocyte-macrophage colony stimulating factor (GM-CSF) were kept above 97%. Low viability losses were observed, as measured by trypan blue exclusion and by determination of intracellular lactate dehydrogenase (LDH) released to the culture medium. Mathematical models were proposed to predict the flow rate, flow ratio and separation efficiency as a function of hydrocyclone geometry and pressure drop. When cells were monitored for any induction of apoptosis upon passage through the hydrocyclones, no increase in apoptotic cell concentration was observed within 48 h of hydrocycloning. Thus, based on the high separation efficiencies, the robustness of the equipment, and the absence of apoptosis induction, hydrocyclones seem to be specially suited for use as cell retention devices in long-term perfusion runs.

Reducing Liquid Loss during Ullage Venting in Microgravity

NASA Technical Reports Server (NTRS)

Nguyen, Bich; Nguyen, Lauren

2008-01-01

A centripetal-force-based liquid/gas separator has been proposed as a means of reducing the loss of liquid during venting of the ullage of a tank in microgravity as a new supply of liquid is pumped into the tank. Centripetal-force-based liquid/gas separators are used on Earth, where mechanical drives (e.g., pumps and spinners) are used to impart flow speeds sufficient to generate centripetal forces large enough to effect separation of liquids from gases. For the proposed application, the separator would be designed so that there would be no need for such a pump because the tank-pressure-induced outflow speed during venting of the ullage would be sufficient for centripetal separation. A relatively small pump would be used, not for separation, but for returning the liquid recovered by the separator to the tank.

[Identifying dry-weather flow and pollution load sources of separate storm sewer systems with different degrees of illicit discharge].

PubMed

Meng, Ying-ying; Feng, Cang; Li, Tian; Wang, Ling

2009-12-01

Dry-weather flow quantity and quality of three representative separate storm sewer systems in Shanghai-H, G, N were studied. Based on survey of operating status of the pumping stations as well as characteristics of the drainage systems, it was obtained that the interception sewage volumes per unit area in the three systems were 3610 m3/(km2 x d), 1550 m3/(km2 x d), 2970 m3/(km2 x d) respectively; the sanitary wastewater included accounted for 25%, 85% and 71% respectively; the interception volume of H was mainly composed of infiltrated underground water, so the dry-weather flow pollution was slighter, and the interception volumes of G, N were both mainly composed of sanitary wastewater, so the dry-weather which were flow pollution was relatively serious. The water characteristics of potential illicit discharge sources of dry-weather which were flow-grey water, black water and underground water were preliminarily explored, so that treating three parameters-LAS/ NH4+ -N, NH4+ -N/K, Mg/K as tracer parameters of grey water, black water and underground water was put forward. Moreover, the water characteristics of grey water and sanitary wastewater including black water were summarized: the feature of grey water was LAS/NH4+ -N > 0.2, NH4+ -N/K <1, and sanitary wastewater was LAS/NH4+ -N < 0.2, NH4+ -N/K >1. Based on the above, the applications of flow chart method and CMBM method in dry-weather flow detection of monitored storm systems were preliminarily discussed, and the results were basically same as that obtained in flow quantity and quality comprehensive analysis. The research results and methods can provide guidance for analysis and diagnosis of dry-weather flow sources and subsequent reconstruction projects in similar separate storm sewer systems at home.

Surface-Micromachined Microfluidic Devices

DOEpatents

Galambos, Paul C.; Okandan, Murat; Montague, Stephen; Smith, James H.; Paul, Phillip H.; Krygowski, Thomas W.; Allen, James J.; Nichols, Christopher A.; Jakubczak, II, Jerome F.

2004-09-28

Microfluidic devices are disclosed which can be manufactured using surface-micromachining. These devices utilize an electroosmotic force or an electromagnetic field to generate a flow of a fluid in a microchannel that is lined, at least in part, with silicon nitride. Additional electrodes can be provided within or about the microchannel for separating particular constituents in the fluid during the flow based on charge state or magnetic moment. The fluid can also be pressurized in the channel. The present invention has many different applications including electrokinetic pumping, chemical and biochemical analysis (e.g. based on electrophoresis or chromatography), conducting chemical reactions on a microscopic scale, and forming hydraulic actuators. Microfluidic devices are disclosed which can be manufactured using surface-micromachining. These devices utilize an electroosmotic force or an electromagnetic field to generate a flow of a fluid in a microchannel that is lined, at least in part, with silicon nitride. Additional electrodes can be provided within or about the microchannel for separating particular constituents in the fluid during the flow based on charge state or magnetic moment. The fluid can also be pressurized in the channel. The present invention has many different applications including electrokinetic pumping, chemical and biochemical analysis (e.g. based on electrophoresis or chromatography), conducting chemical reactions on a microscopic scale, and forming hydraulic actuators.

Cellular Structures in the Flow Over the Flap of a Two-Element Wing

NASA Technical Reports Server (NTRS)

Yon, Steven A.; Katz, Joseph

1997-01-01

Flow visualization information and time dependent pressure coefficients were recorded for the flow over a two-element wing. The investigation focused on the stall onset; particularly at a condition where the flow is attached on the main element but separated on the flap. At this condition, spanwise separation cells were visible in the flow over the flap, and time dependent pressure data was measured along the centerline of the separation cell. The flow visualizations indicated that the spanwise occurrence of the separation cells depends on the flap (and not wing) aspect ratio.

Velocity-Field Measurements of an Axisymmetric Separated Flow Subjected to Amplitude-Modulated Excitation

NASA Technical Reports Server (NTRS)

Trosin, Barry James

2007-01-01

Active flow control was applied at the point of separation of an axisymmetric, backward-facing-step flow. The control was implemented by employing a Helmholtz resonator that was externally driven by an amplitude-modulated, acoustic disturbance from a speaker located upstream of the wind tunnel. The velocity field of the separating/reattaching flow region downstream of the step was characterized using hotwire velocity measurements with and without flow control. Conventional statistics of the data reveal that the separating/reattaching flow is affected by the imposed forcing. Triple decomposition along with conditional averaging was used to distinguish periodic disturbances from random turbulence in the fluctuating velocity component. A significant outcome of the present study is that it demonstrates that amplitude-modulated forcing of the separated flow alters the flow in the same manner as the more conventional method of periodic excitation.

Separation of Single-Walled Carbon Nanotubes with DEP-FFF

NASA Technical Reports Server (NTRS)

Schmidt, Howard K.; Peng, Haiqing; Alvarez, Noe; Mendes, Manuel; Pasquali, Matteo

2011-01-01

A process using a modified dielectrophoresis device separates single-walled carbon nanotubes (SWNTs) according to their polarizability in electric fields. This depends on the size and dielectric constant of individual nanotubes and easily separates metallic from semiconducting nanotubes. Separation by length has also been demonstrated. Partial separation (enrichment) according to bandgap (which is linked to polarizability) has also been shown and can be improved to full separation of individual types of semiconducting SWNTs with better control over operational parameters and the length of SWNT starting material. This process and device can be scaled affordably to generate useful amounts of semiconducting SWNTs for electronic device development and production. In this study, a flow injection dielectrophoresis technique was used with a modified dielectrophoresis device. The length, width, and height of the modified chamber were 28, 2.5, and 0.025 cm, respectively. On the bottom of the chamber, there are two arrays of 50-m-wide, 2-m-thick gold electrodes, which are connected to an AC voltage generator and are alternately arranged so that every electrode is adjacent to two electrodes of the opposite polar. There is an additional plate electrode on the top of the chamber that is negatively biased. During the experiment, a syringe pump constantly pumps in the mobile phase, 1-percent sodium dodecylbenzene sulfonate (SDBS) solution, into the chamber. The frequency and voltage are set to 1 MHz and 10 V peak-to-peak, respectively. About 150 micro-L of SWNTs in 1- percent SDBS decanted solution are injected to the mobile phase through a septum near the entrance of the chamber. The flow rate of the mobile phase is set to 0.02 cu cm/min. The injected SWNTs sample flows through the chamber before it is lead into a fluorescence flow-through cell and collected for further analysis. The flow-through cell has three windows, thus allowing the fluorometer to collect fluorescence spectrum and visible absorption spectrums simultaneously. Dielectrophoresis field-flow fractionation (DEP-FFF) generally depends on interaction of a sedimentation force and DEP force for particle separation, and SWNTs are neutrally buoyant in water. In this innovation, the third electrode was added to create a sedimentation force based on DC electrophoresis. This makes this particular device applicable to separations on any neutrally buoyant particles in solution and a more general process for a broad range of nanomaterials sorting and separations.

A two-stage flow-based intrusion detection model for next-generation networks.

PubMed

Umer, Muhammad Fahad; Sher, Muhammad; Bi, Yaxin

2018-01-01

The next-generation network provides state-of-the-art access-independent services over converged mobile and fixed networks. Security in the converged network environment is a major challenge. Traditional packet and protocol-based intrusion detection techniques cannot be used in next-generation networks due to slow throughput, low accuracy and their inability to inspect encrypted payload. An alternative solution for protection of next-generation networks is to use network flow records for detection of malicious activity in the network traffic. The network flow records are independent of access networks and user applications. In this paper, we propose a two-stage flow-based intrusion detection system for next-generation networks. The first stage uses an enhanced unsupervised one-class support vector machine which separates malicious flows from normal network traffic. The second stage uses a self-organizing map which automatically groups malicious flows into different alert clusters. We validated the proposed approach on two flow-based datasets and obtained promising results.

A two-stage flow-based intrusion detection model for next-generation networks

PubMed Central

2018-01-01

The next-generation network provides state-of-the-art access-independent services over converged mobile and fixed networks. Security in the converged network environment is a major challenge. Traditional packet and protocol-based intrusion detection techniques cannot be used in next-generation networks due to slow throughput, low accuracy and their inability to inspect encrypted payload. An alternative solution for protection of next-generation networks is to use network flow records for detection of malicious activity in the network traffic. The network flow records are independent of access networks and user applications. In this paper, we propose a two-stage flow-based intrusion detection system for next-generation networks. The first stage uses an enhanced unsupervised one-class support vector machine which separates malicious flows from normal network traffic. The second stage uses a self-organizing map which automatically groups malicious flows into different alert clusters. We validated the proposed approach on two flow-based datasets and obtained promising results. PMID:29329294

Turbulent transition behavior in a separated and attached-flow low pressure turbine passage

NASA Astrophysics Data System (ADS)

Memory, Curtis L.

Various time accurate numerical simulations were conducted on the aft-loaded L1A low pressure turbine airfoil operating at Reynolds numbers presenting with fully-stalled, non-reattaching laminar separation. The numerical solver TURBO was modified from its annular gas turbine simulation configuration to conduct simulations based on a linear cascade wind tunnel facility. Simulation results for the fully separated flow fields revealed various turbulent decay mechanisms. Separated shear layer decay, in the form of vortices forming between the shear layer and the blade wall, was shown to agree with experimental particle image velocimetry (PIV) data in terms of decay vortex size and core vorticity levels. These vortical structures eventually mix into a large recirculation zone which dominates the blade wake. Turbulent wake ex- tent and time-averaged velocity distributions agreed with PIV data. Steady-blowing vortex generating jet (VGJ) flow control was then applied to the flow fields. VGJ-induced streamwise vorticity was only present at blowing ratios above 1.5. VGJs actuated at the point of flow separation on the blade wall were more effective than those actuated downstream, within the separation zone. Pulsed-blowing VGJs at the upstream blade wall position were then actuated at various pulsing frequencies, duty cycles, and blowing ratios. These condition variations yielded differing levels of separation zone mitigation. Pulsed VGJs were shown to be more effective than steady blowing VGJs at conditions of high blowing ratio, high frequency, or high duty cycle, where blowing ratio had the highest level of influence on pulsed jet efficacy. The characteristic "calm zone" following the end of a given VGJ pulse was observed in simulations exhibiting high levels of separation zone mitigation. Numerical velocity fields near the blade wall during this calm zone was shown to be similar to velocity fields observed in PIV data. Instantaneous numerical vorticity fields indicated that the elimination of the separation zone directly downstream of the VGJ hole is a pri- mary indicator of pulsed VGJ efficacy. This indicator was confirmed by numerical time-averaged velocity magnitude rms data in the same region.

Visualization of entry flow separation for oscillating flow in tubes

NASA Technical Reports Server (NTRS)

Qiu, Songgang; Simon, Terence W.

1992-01-01

Neutrally buoyant helium-filled soap bubbles with laser illumination are used to document entry flow separation for oscillating flow in tubes. For a symmetric entry case, the size of the separation zone appears to mildly depend on Reynolds number in the acceleration phase, but is roughly Reynolds number independent in the deceleration phase. For the asymmetric entry case, the separation zone was larger and appeared to grow somewhat during the deceleration phase. The separation zones for both entry geometry cases remain relatively small throughout the cycle. This is different from what would be observed in all-laminar, oscillator flows and is probably due to the high turbulence of the flow, particularly during the deceleration phase of the cycle.

Removing Grit During Wastewater Treatment: CFD Analysis of HDVS Performance.

PubMed

Meroney, Robert N; Sheker, Robert E

2016-05-01

Computational Fluid Dynamics (CFD) was used to simulate the grit and sand separation effectiveness of a typical hydrodynamic vortex separator (HDVS) system. The analysis examined the influences on the separator efficiency of: flow rate, fluid viscosities, total suspended solids (TSS), and particle size and distribution. It was found that separator efficiency for a wide range of these independent variables could be consolidated into a few curves based on the particle fall velocity to separator inflow velocity ratio, Ws/Vin. Based on CFD analysis it was also determined that systems of different sizes with length scale ratios ranging from 1 to 10 performed similarly when Ws/Vin and TSS were held constant. The CFD results have also been compared to a limited range of experimental data.

Surface flow visualization of separated flows on the forebody of an F-18 aircraft and wind-tunnel model

NASA Technical Reports Server (NTRS)

Fisher, David F.; Richwine, David M.; Banks, Daniel W.

1988-01-01

A method of in-flight surface flow visualization similar to wind-tunnel-model oil flows is described for cases where photo-chase planes or onboard photography are not practical. This method, used on an F-18 aircraft in flight at high angles of attack, clearly showed surface flow streamlines in the fuselage forebody. Vortex separation and reattachment lines were identified with this method and documented using postflight photography. Surface flow angles measured at the 90 and 270 degrees meridians show excellent agreement with the wind tunnel data for a pointed tangent ogive with an aspect ratio of 3.5. The separation and reattachment line locations were qualitatively similar to the F-18 wind-tunnel-model oil flows but neither the laminar separation bubble nor the boundary-layer transition on the wind tunnel model were evident in the flight surface flows. The separation and reattachment line locations were in fair agreement with the wind tunnel data for the 3.5 ogive. The elliptical forebody shape of the F-18 caused the primary separation lines to move toward the leeward meridian. Little effect of angle of attack on the separation locations was noted for the range reported.

Documentation and Control of Flow Separation on a Low Pressure Turbine Linear Cascade of Pak-B Blades Using Plasma Actuators

NASA Technical Reports Server (NTRS)

Corke, Thomas c.; Thomas, FLint, O.; Huang, Junhui

2007-01-01

This work involved the documentation and control of flow separation that occurs over low pressure turbine (LPT) blades at low Reynolds numbers. A specially constructed linear cascade was utilized to study the flow field over a generic LPT cascade consisting of Pratt & Whitney "Pak-B" shaped blades. Flow visualization, surface pressure measurements, LDV measurements, and hot-wire anemometry were conducted to examine the flow fields with and without separation control. Experimental conditions were chosen to give a range of chord Reynolds numbers (based on axial chord and inlet velocity) from 10,000 to 100,000, and a range of freestream turbulence intensities from u'/U(infinity) = 0.08 to 2.85 percent. The blade pressure distributions were measured and used to identify the region of separation that depends on Reynolds number and the turbulence intensity. Separation control was performed using dielectric barrier discharge (DBD) plasma actuators. Both steady and unsteady actuation were implemented and found to work well. The comparison between the steady and unsteady actuators showed that the unsteady actuators worked better than the steady ones. For the steady actuators, it was found that the separated region is significantly reduced. For the unsteady actuators, where the signal was pulsed, the separation was eliminated. The total pressure losses (a low Reynolds number) was reduced by approximately a factor of two. It was also found that lowest plasma duty cycle (10 percent in this work) was as effective as the highest plasma duty cycle (50 percent in this work). The mechanisms of the steady and unsteady plasma actuators were studied. It was suggested by the experimental results that the mechanism for the steady actuators is turbulence tripping, while the mechanism for the unsteady actuators is to generate a train of spanwise structures that promote mixing.

A novel mechanical model for phase-separation in debris flows

NASA Astrophysics Data System (ADS)

Pudasaini, Shiva P.

2015-04-01

Understanding the physics of phase-separation between solid and fluid phases as a two-phase mass moves down slope is a long-standing challenge. Here, I propose a fundamentally new mechanism, called 'separation-flux', that leads to strong phase-separation in avalanche and debris flows. This new model extends the general two-phase debris flow model (Pudasaini, 2012) to include a separation-flux mechanism. The new flux separation mechanism is capable of describing and controlling the dynamically evolving phase-separation, segregation, and/or levee formation in a real two-phase, geometrically three-dimensional debris flow motion and deposition. These are often observed phenomena in natural debris flows and industrial processes that involve the transportation of particulate solid-fluid mixture material. The novel separation-flux model includes several dominant physical and mechanical aspects that result in strong phase-separation (segregation). These include pressure gradients, volume fractions of solid and fluid phases and their gradients, shear-rates, flow depth, material friction, viscosity, material densities, boundary structures, gravity and topographic constraints, grain shape, size, etc. Due to the inherent separation mechanism, as the mass moves down slope, more and more solid particles are brought to the front, resulting in a solid-rich and mechanically strong frontal surge head followed by a weak tail largely consisting of the viscous fluid. The primary frontal surge head followed by secondary surge is the consequence of the phase-separation. Such typical and dominant phase-separation phenomena are revealed here for the first time in real two-phase debris flow modeling and simulations. However, these phenomena may depend on the bulk material composition and the applied forces. Reference: Pudasaini, Shiva P. (2012): A general two-phase debris flow model. J. Geophys. Res., 117, F03010, doi: 10.1029/2011JF002186.

A multi-block adaptive solving technique based on lattice Boltzmann method

NASA Astrophysics Data System (ADS)

Zhang, Yang; Xie, Jiahua; Li, Xiaoyue; Ma, Zhenghai; Zou, Jianfeng; Zheng, Yao

2018-05-01

In this paper, a CFD parallel adaptive algorithm is self-developed by combining the multi-block Lattice Boltzmann Method (LBM) with Adaptive Mesh Refinement (AMR). The mesh refinement criterion of this algorithm is based on the density, velocity and vortices of the flow field. The refined grid boundary is obtained by extending outward half a ghost cell from the coarse grid boundary, which makes the adaptive mesh more compact and the boundary treatment more convenient. Two numerical examples of the backward step flow separation and the unsteady flow around circular cylinder demonstrate the vortex structure of the cold flow field accurately and specifically.

Numerical simulation of base flow of a long range flight vehicle

NASA Astrophysics Data System (ADS)

Saha, S.; Rathod, S.; Chandra Murty, M. S. R.; Sinha, P. K.; Chakraborty, Debasis

2012-05-01

Numerical exploration of base flow of a long range flight vehicle is presented for different flight conditions. Three dimensional Navier-Stokes equations are solved along with k-ɛ turbulence model using commercial CFD software. Simulation captured all essential flow features including flow separation at base shoulder, shear layer formation at the jet boundary, recirculation at the base region etc. With the increase in altitude, the plume of the rocket exhaust is seen to bulge more and more and caused more intense free stream and rocket plume interaction leading to higher gas temperature in the base cavity. The flow field in the base cavity is investigated in more detail, which is found to be fairly uniform at different instant of time. Presence of the heat shield is seen to reduce the hot gas entry to the cavity region due to different recirculation pattern in the base region. Computed temperature history obtained from conjugate heat transfer analysis is found to compare very well with flight measured data.

A Navier-Stokes Solution of Hull-Ring Wing-Thruster Interaction

NASA Technical Reports Server (NTRS)

Yang, C.-I.; Hartwich, P.; Sundaram, P.

1991-01-01

Navier-Stokes simulations of high Reynolds number flow around an axisymmetric body supported in a water tunnel were made. The numerical method is based on a finite-differencing high resolution second-order accurate implicit upwind scheme. Four different configurations were investigated, these are: (1) barebody; (2) body with an operating propeller; (3) body with a ring wing; and (4) body with a ring wing and an operating propeller. Pressure and velocity components near the stern region were obtained computationally and are shown to compare favorably with the experimental data. The method correctly predicts the existence and extent of stern flow separation for the barebody and the absence of flow separation for the three other configurations with ring wing and/or propeller.

Proceedings: Joint DOE/NSF Workshop on flow of particulates and fluids

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-12-31

These proceedings are the result of the Fifth DOR-NSF Workshop on fundamental research in the area of particulate two-phase flow and granular flow. The present collection of twenty contributions from universities and national laboratories is based on research projects sponsored by either the Department of Energy or the National Science Foundation. These papers illustrate some of the latest advances in theory, simulations, and experiments. The papers from the Workshop held September 29--October 1, 1993 have been separated into three basic areas: experiments, theory, and numerical simulations. A list of attendees at the workshop is included at the end of themore » proceedings. Selected papers have been indexed separately for inclusion in the Energy Science and Technology Database.« less

An efficient multi-dimensional implementation of VSIAM3 and its applications to free surface flows

NASA Astrophysics Data System (ADS)

Yokoi, Kensuke; Furuichi, Mikito; Sakai, Mikio

2017-12-01

We propose an efficient multidimensional implementation of VSIAM3 (volume/surface integrated average-based multi-moment method). Although VSIAM3 is a highly capable fluid solver based on a multi-moment concept and has been used for a wide variety of fluid problems, VSIAM3 could not simulate some simple benchmark problems well (for instance, lid-driven cavity flows) due to relatively high numerical viscosity. In this paper, we resolve the issue by using the efficient multidimensional approach. The proposed VSIAM3 is shown to capture lid-driven cavity flows of the Reynolds number up to Re = 7500 with a Cartesian grid of 128 × 128, which was not capable for the original VSIAM3. We also tested the proposed framework in free surface flow problems (droplet collision and separation of We = 40 and droplet splashing on a superhydrophobic substrate). The numerical results by the proposed VSIAM3 showed reasonable agreements with these experiments. The proposed VSIAM3 could capture droplet collision and separation of We = 40 with a low numerical resolution (8 meshes for the initial diameter of droplets). We also simulated free surface flows including particles toward non-Newtonian flow applications. These numerical results have showed that the proposed VSIAM3 can robustly simulate interactions among air, particles (solid), and liquid.

Towards a Viscous Wall Model for Immersed Boundary Methods

NASA Technical Reports Server (NTRS)

Brehm, Christoph; Barad, Michael F.; Kiris, Cetin C.

2016-01-01

Immersed boundary methods are frequently employed for simulating flows at low Reynolds numbers or for applications where viscous boundary layer effects can be neglected. The primary shortcoming of Cartesian mesh immersed boundary methods is the inability of efficiently resolving thin turbulent boundary layers in high-Reynolds number flow application. The inefficiency of resolving the thin boundary is associated with the use of constant aspect ratio Cartesian grid cells. Conventional CFD approaches can efficiently resolve the large wall normal gradients by utilizing large aspect ratio cells near the wall. This paper presents different approaches for immersed boundary methods to account for the viscous boundary layer interaction with the flow-field away from the walls. Different wall modeling approaches proposed in previous research studies are addressed and compared to a new integral boundary layer based approach. In contrast to common wall-modeling approaches that usually only utilize local flow information, the integral boundary layer based approach keeps the streamwise history of the boundary layer. This allows the method to remain effective at much larger y+ values than local wall modeling approaches. After a theoretical discussion of the different approaches, the method is applied to increasingly more challenging flow fields including fully attached, separated, and shock-induced separated (laminar and turbulent) flows.

Fuel cell repeater unit including frame and separator plate

DOEpatents

Yamanis, Jean; Hawkes, Justin R; Chiapetta, Jr., Louis; Bird, Connie E; Sun, Ellen Y; Croteau, Paul F

2013-11-05

An example fuel cell repeater includes a separator plate and a frame establishing at least a portion of a flow path that is operative to communicate fuel to or from at least one fuel cell held by the frame relative to the separator plate. The flow path has a perimeter and any fuel within the perimeter flow across the at least one fuel cell in a first direction. The separator plate, the frame, or both establish at least one conduit positioned outside the flow path perimeter. The conduit is outside of the flow path perimeter and is configured to direct flow in a second, different direction. The conduit is fluidly coupled with the flow path.

Vorticity Distributions in Unsteady Flow Separation

DTIC Science & Technology

1988-11-08

a significant result, which was presented at the Unsteady Separated Flow Workshop at the Air Force Academy last July, and which is ready for...i~~A’I C amsi4 61102F 2307 A2 11 Ti-,LE (Incluce Security Claw fication) Vorticity Distributions in Unsteady Flow Separation 12 PERSONAL AUTHOR(S...LSIIAINO HSPG / UNCLASSIFIED Report MEUA-IT-88-2 VORTICITY DISTRIBUTIONS IN UNSTEADY FLOW SEPARATION Frederick S. Sherman Department of Mechanical

Laboratory investigation and direct numerical simulation of wind effect on steep surface waves

NASA Astrophysics Data System (ADS)

Troitskaya, Yuliya; Sergeev, Daniil; Druzhinin, Oleg; Ermakova, Olga

2015-04-01

The small scale ocean-atmosphere interaction at the water-air interface is one of the most important factors determining the processes of heat, mass, and energy exchange in the boundary layers of both geospheres. Another important aspect of the air-sea interaction is excitation of surface waves. One of the most debated open questions of wave modeling is concerned with the wind input in the wave field, especially for the case of steep and breaking waves. Two physical mechanisms are suggested to describe the excitation of finite amplitude waves. The first one is based on the treatment of the wind-wave interaction in quasi-linear approximation in the frameworks of semi-empirical models of turbulence of the low atmospheric boundary layer. An alternative mechanism is associated with separation of wind flow at the crests of the surface waves. The "separating" and "non-separating" mechanisms of wave generation lead to different dependences of the wind growth rate on the wave steepness: the latter predicts a decrease in the increment with wave steepness, and the former - an increase. In this paper the mechanism of the wind-wave interaction is investigated basing on physical and numerical experiments. In the physical experiment, turbulent airflow over waves was studied using the video-PIV method, based on the application of high-speed video photography. Alternatively to the classical PIV technique this approach provides the statistical ensembles of realizations of instantaneous velocity fields. Experiments were performed in a round wind-wave channel at Institute of Applied Physics, Russian Academy of Sciences. A fan generated the airflow with the centerline velocity 4 m/s. The surface waves were generated by a programmed wave-maker at the frequency of 2.5 Hz with the amplitudes of 0.65 cm, 1.4 cm, and 2 cm. The working area (27.4 × 10.7 cm2) was at a distance of 3 m from the fan. To perform the measurements of the instantaneous velocity fields, spherical polyamide particles 20 μm in diameter were injected into the airflow. The images of the illuminated particles were photographed with a digital CCD video camera at a rate of 1000 frames per second. For the each given parameters of wind and waves, a statistical ensemble of 30 movies with duration from 200 to 600 ms was obtained. Individual flow realizations manifested the typical features of flow separation, while the average vector velocity fields obtained by the phase averaging of the individual vector fields were smooth and slightly asymmetrical, with the minimum of the horizontal velocity near the water surface shifted to the leeward side of the wave profile, but do not demonstrate the features of flow separation. The wave-induced pressure perturbations, averaged over the turbulent fluctuations, were retrieved from the measured velocity fields, using the Reynolds equations. It ensures sufficient accuracy for study of the dependence of the wave increment on the wave amplitude. The dependences of the wave growth rate on the wave steepness are weakly decreasing, serving as indirect proof of the non-separated character of flow over waves. Also direct numerical simulation of the airflow over finite amplitude periodic surface wave was performed. In the experiments the primitive 3-dimensional fluid mechanics equations were solved in the airflow over curved water boundary for the following parameters: the Reynolds number Re=15000, the wave steepness ka=0-0.2, the parameter c/u*=0-10 (where u* is the friction velocity and c is the wave celerity). Similar to the physical experiment the instant realizations of the velocity field demonstrate flow separation at the crests of the waves, but the ensemble averaged velocity fields had typical structures similar to those excising in shear flows near critical levels, where the phase velocity of the disturbance coincides with the flow velocity. The wind growth rate determined by the ensemble averaged wave-induced pressure component in phase of the wave slope was retrieved from the DNS results. Similar to the physical experiment the wave growth rate weakly decreased with the wave steepness. The results of physical and numerical experiments were compared with the calculations within the theoretical model of a turbulent boundary layer based on the system of Reynolds equations with the first-order closing hypothesis. Within the model the wind-wave interaction is considered within the quasi-linear approximation and the mean airflow over waves within the model is treated as a non-separated. The calculations within the model represents well profiles of the mean wind velocity, turbulent stress, amplitude and phase of the main harmonics of the wave-induced velocity components and also wave-induced pressure fluctuations and wind wave growth rate obtained both in the physical experiment and DNS. Applicability of the non-separating quasi-linear theory for description of average fields in the airflow over steep and even breaking waves, when the effect of separation is manifested in the instantaneous flow images, can possibly be explained qualitatively by the strongly non-stationary character of the separation process with the typical time being much less than the wave period, and by the small scale of flow heterogeneity in the area of separation. In such a situation small-scale vortices produced within the separation bubble affect the mean flow and wind-induced disturbances as eddy viscosity. Then, the flow turbulence affects the averaged fields as a very viscous fluid, where the effective Reynolds number for the average fields determined by the eddy viscosity was small even for steep waves. It follows from this assumption that strongly nonlinear effects, such as flow separations should not be expected in the flow averaged over turbulent fluctuations, and the main harmonics of the wave-induced disturbances of the averaged flow, which determine the energy flux to surface waves, can be described in the weakly-nonlinear approximation. This paper was supported by a grant from the Government of the Russian Federation under Contract no. 11.G34.31.0048; the European Research Council Advanced Grant, FP7-IDEAS, 227915; RFBF grant 13-05-00865-а, 13-05-12093-ofi-m,15-05-91767.

40 CFR 98.443 - Calculating CO2 geologic sequestration.

Code of Federal Regulations, 2011 CFR

2011-07-01

... each gas-liquid separator for which flow is measured using a mass flow meter, you must calculate...) For each gas-liquid separator for which flow is measured using a volumetric flow meter, you must...) To aggregate production data, you must sum the mass of all of the CO2 separated at each gas-liquid...

Three-dimensional numerical study of heat transfer enhancement in separated flows

NASA Astrophysics Data System (ADS)

Kumar, Saurav; Vengadesan, S.

2017-11-01

The flow separation appears in a wide range of heat transfer applications and causes poor heat transfer performance. It motivates the study of heat transfer enhancement in laminar as well as turbulent flows over a backward facing step by means of an adiabatic fin mounted on the top wall. Recently, we have studied steady, 2-D numerical simulations in laminar flow and investigated the effect of fin length, location, and orientation. It revealed that the addition of fin causes enhancement of heat transfer and it is very effective to control the flow and thermal behavior. The fin is most effective and sensitive when it is placed exactly above the step. A slight displacement of the fin in upstream of the step causes the complete change of flow and thermal behavior. Based on the obtained 2-D results it is interesting to investigate the side wall effect in three-dimensional simulations. The comparison of two-dimensional and three-dimensional numerical simulations with the available experimental results will be presented. Special attention has to be given to capture unsteadiness in the flow and thermal field.

Numerical computation of viscous flow about unconventional airfoil shapes

NASA Technical Reports Server (NTRS)

Ahmed, S.; Tannehill, J. C.

1990-01-01

A new two-dimensional computer code was developed to analyze the viscous flow around unconventional airfoils at various Mach numbers and angles of attack. The Navier-Stokes equations are solved using an implicit, upwind, finite-volume scheme. Both laminar and turbulent flows can be computed. A new nonequilibrium turbulence closure model was developed for computing turbulent flows. This two-layer eddy viscosity model was motivated by the success of the Johnson-King model in separated flow regions. The influence of history effects are described by an ordinary differential equation developed from the turbulent kinetic energy equation. The performance of the present code was evaluated by solving the flow around three airfoils using the Reynolds time-averaged Navier-Stokes equations. Excellent results were obtained for both attached and separated flows about the NACA 0012 airfoil, the RAE 2822 airfoil, and the Integrated Technology A 153W airfoil. Based on the comparison of the numerical solutions with the available experimental data, it is concluded that the present code in conjunction with the new nonequilibrium turbulence model gives excellent results.

Asymmetric flow field flow fractionation with light scattering detection - an orthogonal sensitivity analysis.

PubMed

Galyean, Anne A; Filliben, James J; Holbrook, R David; Vreeland, Wyatt N; Weinberg, Howard S

2016-11-18

Asymmetric flow field flow fractionation (AF 4 ) has several instrumental factors that may have a direct effect on separation performance. A sensitivity analysis was applied to ascertain the relative importance of AF 4 primary instrument factor settings for the separation of a complex environmental sample. The analysis evaluated the impact of instrumental factors namely, cross flow, ramp time, focus flow, injection volume, and run buffer concentration on the multi-angle light scattering measurement of natural organic matter (NOM) molar mass (MM). A 2 (5-1) orthogonal fractional factorial design was used to minimize analysis time while preserving the accuracy and robustness in the determination of the main effects and interactions between any two instrumental factors. By assuming that separations resulting in smaller MM measurements would be more accurate, the analysis produced a ranked list of effects estimates for factors and interactions of factors based on their relative importance in minimizing the MM. The most important and statistically significant AF 4 instrumental factors were buffer concentration and cross flow. The least important was ramp time. A parallel 2 (5-2) orthogonal fractional factorial design was also employed on five environmental factors for synthetic natural water samples containing silver nanoparticles (NPs), namely: NP concentration, NP size, NOM concentration, specific conductance, and pH. None of the water quality characteristic effects or interactions were found to be significant in minimizing the measured MM; however, the interaction between NP concentration and NP size was an important effect when considering NOM recovery. This work presents a structured approach for the rigorous assessment of AF 4 instrument factors and optimal settings for the separation of complex samples utilizing efficient orthogonal factional factorial design and appropriate graphical analysis. Copyright © 2016 Elsevier B.V. All rights reserved.

Ground-water recharge from streamflow data, NW Florida

USGS Publications Warehouse

Vecchioli, John; Bridges, W.C.; Rumenik, Roger P.; Grubbs, J.W.

1991-01-01

Annual base flows of streams draining Okaloosa County and adjacent areas in northwest Florida were determined through hydrograph separation and correlation techniques for purposes of evaluating variations in ground-water recharge rates. Base flows were least in the northern part of the county and greatest in the southern part. Topographic and soils data were then superimposed on the distribution of base flow by subbasin to produce a map showing distribution of ground-water recharge throughout the county. The highest recharge rate occurs in the southern part of the county where relatively flat upland areas underlain by excessively drained sandy soils result in minimal storm runoff and evapotranspiration.

Particle Image Velocimetry Measurements of a Two/Three-dimensional Separating/Reattaching Boundary Layer Downstream of an Axisymmetric Backward-facing Step

NASA Technical Reports Server (NTRS)

Hudy, Laura M.; Naguib, Ahmed M.; Humphreys, William M.; Bartram, Scott M.

2005-01-01

Planar Particle Image Velocimetry measurements were obtained in the separating/reattaching flow region downstream of an axisymmetric backward-facing step. Data were acquired for a two-dimensional (2D) separating boundary layer at five different Reynolds numbers based on step height (Re(sub h)), spanning 5900-33000, and for a three-dimensional (3D) separating boundary layer at Re(sub h) = 5980 and 8081. Reynolds number effects were investigated in the 2D cases using mean-velocity field, streamwise and wall-normal turbulent velocity, and Reynolds stress statistics. Results show that both the reattachment length (x(sub r)) and the secondary separation point are Reynolds number dependent. The reattachment length increased with rising Re(sub h) while the secondary recirculation region decreased in size. These and other Re(sub h) effects were interpreted in terms of changes in the separating boundary layer thickness and wall-shear stress. On the other hand, in the 3D case, it was found that the imposed cross-flow component was relatively weak in comparison to the streamwise component. As a result, the primary influences of three dimensionality only affected the near-separation region rather than the entire separation bubble.

Classification of California streams using combined deductive and inductive approaches: Setting the foundation for analysis of hydrologic alteration

USGS Publications Warehouse

Pyne, Matthew I.; Carlisle, Daren M.; Konrad, Christopher P.; Stein, Eric D.

2017-01-01

Regional classification of streams is an early step in the Ecological Limits of Hydrologic Alteration framework. Many stream classifications are based on an inductive approach using hydrologic data from minimally disturbed basins, but this approach may underrepresent streams from heavily disturbed basins or sparsely gaged arid regions. An alternative is a deductive approach, using watershed climate, land use, and geomorphology to classify streams, but this approach may miss important hydrological characteristics of streams. We classified all stream reaches in California using both approaches. First, we used Bayesian and hierarchical clustering to classify reaches according to watershed characteristics. Streams were clustered into seven classes according to elevation, sedimentary rock, and winter precipitation. Permutation-based analysis of variance and random forest analyses were used to determine which hydrologic variables best separate streams into their respective classes. Stream typology (i.e., the class that a stream reach is assigned to) is shaped mainly by patterns of high and mean flow behavior within the stream's landscape context. Additionally, random forest was used to determine which hydrologic variables best separate minimally disturbed reference streams from non-reference streams in each of the seven classes. In contrast to stream typology, deviation from reference conditions is more difficult to detect and is largely defined by changes in low-flow variables, average daily flow, and duration of flow. Our combined deductive/inductive approach allows us to estimate flow under minimally disturbed conditions based on the deductive analysis and compare to measured flow based on the inductive analysis in order to estimate hydrologic change.

Experimental Study of Unsteady Flow Separation in a Laminar Boundary Layer

NASA Astrophysics Data System (ADS)

Bonacci, Andrew; Lang, Amy; Wahidi, Redha; Santos, Leonardo

2017-11-01

Flow separation, caused by an adverse pressure gradient, is a major problem in many applications. Reversing flow near the wall is the first sign of incipient separation and can bristle shark scales which may be linked to a passive, flow actuated separation control mechanism. An investigation of how this backflow forms and how it interacts with shark skin is of interest due to the fact that this could be used as a bioinspired means of initiating flow control. A water tunnel experiment aims to study unsteady separation with a focus on the reversing flow development near the wall within a flat plate laminar boundary layer (Re on order of 105) as an increasing adverse pressure gradient is induced by a rotating cylinder. Unsteady reversing flow development is documented using DPIV. Funding was provided by the National Science Foundation under the Research Experience for Undergraduates (REU) program (EEC 1659710) and the Army Research Office.

Turbulent Flow Modification With Thermoacoustic Waves for Separation Control

DTIC Science & Technology

2017-08-24

analyses using two different approaches in order to provide guidance to physics-based design of active flow control using thermal-based actuators. RPPR... control effects are also observed by Post & Corke (2004) on the same airfoil. The uses of plasma actuators on other shear layer setups have been...region may be a more practical approach than introducing control inputs externally. On the other hand, Barone & Lele (2005) studied the receptivity of the

Enhancing resolution of free-flow zone electrophoresis via a simple sheath-flow sample injection.

PubMed

Yang, Ying; Kong, Fan-Zhi; Liu, Ji; Li, Jun-Min; Liu, Xiao-Ping; Li, Guo-Qing; Wang, Ju-Fang; Xiao, Hua; Fan, Liu-Yin; Cao, Cheng-Xi; Li, Shan

2016-07-01

In this work, a simple and novel sheath-flow sample injection method (SFSIM) is introduced to reduce the band broadening of free-flow zone electrophoresis separation in newly developed self-balance free-flow electrophoresis instrument. A needle injector was placed in the center of the separation inlet, into which the BGE and sample solution were pumped simultaneously. BGE formed sheath flow outside the sample stream, resulting in less band broadening related to hydrodynamics and electrodynamics. Hemoglobin and C-phycocyanin were successfully separated by the proposed method in contrast to the poor separation of free-flow electrophoresis with the traditional injection method without sheath flow. About 3.75 times resolution enhancement could be achieved by sheath-flow sample injection method. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Fluid Dynamic Mechanisms and Interactions within Separated Flows.

DTIC Science & Technology

1986-07-01

Vol. 42, Series E, No., pp. 197, pp. 387-39S. b5-bD, March N95, 18. Warpinski , N. R., and Chow, W. L., "Base Pres- 27. Chow, W. L., "Base Pressure of a...lied Rocket/Plume Fluid Dynamic Interactions, Vol. Mechanics, Vol. 46, No. 3, Sept. 197. 1, Base Flows, Fluid Dynamic Lab Report 63-101, 19. Warpinski ...34Surface Pressure Measurements ’" Warpinski , N. R. and Chow, W. L., "Base Pressure Associated on a Boattailed Projectile Shape at Transonic Speeds," ARBRL

High Speed Size Sorting of Subcellular Organelles by Flow Field-Flow Fractionation.

PubMed

Yang, Joon Seon; Lee, Ju Yong; Moon, Myeong Hee

2015-06-16

Separation/isolation of subcellular species, such as mitochondria, lysosomes, peroxisomes, Golgi apparatus, and others, from cells is important for gaining an understanding of the cellular functions performed by specific organelles. This study introduces a high speed, semipreparative scale, biocompatible size sorting method for the isolation of subcellular organelle species from homogenate mixtures of HEK 293T cells using flow field-flow fractionation (FlFFF). Separation of organelles was achieved using asymmetrical FlFFF (AF4) channel system at the steric/hyperlayer mode in which nuclei, lysosomes, mitochondria, and peroxisomes were separated in a decreasing order of hydrodynamic diameter without complicated preprocessing steps. Fractions in which organelles were not clearly separated were reinjected to AF4 for a finer separation using the normal mode, in which smaller sized species can be well fractionated by an increasing order of diameter. The subcellular species contained in collected AF4 fractions were examined with scanning electron microscopy to evaluate their size and morphology, Western blot analysis using organelle specific markers was used for organelle confirmation, and proteomic analysis was performed with nanoflow liquid chromatography-tandem mass spectrometry (nLC-ESI-MS/MS). Since FlFFF operates with biocompatible buffer solutions, it offers great flexibility in handling subcellular components without relying on a high concentration sucrose solution for centrifugation or affinity- or fluorescence tag-based sorting methods. Consequently, the current study provides an alternative, competitive method for the isolation/purification of subcellular organelle species in their intact states.

Optimization and evaluation of asymmetric flow field-flow fractionation of silver nanoparticles.

PubMed

Loeschner, Katrin; Navratilova, Jana; Legros, Samuel; Wagner, Stephan; Grombe, Ringo; Snell, James; von der Kammer, Frank; Larsen, Erik H

2013-01-11

Asymmetric flow field-flow fractionation (AF(4)) in combination with on-line optical detection and mass spectrometry is one of the most promising methods for separation and quantification of nanoparticles (NPs) in complex matrices including food. However, to obtain meaningful results regarding especially the NP size distribution a number of parameters influencing the separation need to be optimized. This paper describes the development of a separation method for polyvinylpyrrolidone-stabilized silver nanoparticles (AgNPs) in aqueous suspension. Carrier liquid composition, membrane material, cross flow rate and spacer height were shown to have a significant influence on the recoveries and retention times of the nanoparticles. Focus time and focus flow rate were optimized with regard to minimum elution of AgNPs in the void volume. The developed method was successfully tested for injected masses of AgNPs from 0.2 to 5.0 μg. The on-line combination of AF(4) with detection methods including ICP-MS, light absorbance and light scattering was helpful because each detector provided different types of information about the eluting NP fraction. Differences in the time-resolved appearance of the signals obtained by the three detection methods were explained based on the physical origin of the signal. Two different approaches for conversion of retention times of AgNPs to their corresponding sizes and size distributions were tested and compared, namely size calibration with polystyrene nanoparticles (PSNPs) and calculations of size based on AF(4) theory. Fraction collection followed by transmission electron microscopy was performed to confirm the obtained size distributions and to obtain further information regarding the AgNP shape. Characteristics of the absorbance spectra were used to confirm the presence of non-spherical AgNP. Copyright © 2012 Elsevier B.V. All rights reserved.

Impact of L/D on 90 Degree Sharp-Edge Orifice Flow with Manifold Passage Cross Flow (Preprint)

DTIC Science & Technology

2007-04-30

that are observed by measurement as the flow transitions from non-cavitation to cavitation (turbulent flow), supercavitation , and finally separation in...include inception of cavitation, supercavitation , and separation. 15. SUBJECT TERMS 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF...cavitation to cavitation (turbulent flow), supercavitation , and finally separation in sharp-edge 90 degree orifices. This study includes orifice L/D from

Speckle variance optical coherence tomography of blood flow in the beating mouse embryonic heart.

PubMed

Grishina, Olga A; Wang, Shang; Larina, Irina V

2017-05-01

Efficient separation of blood and cardiac wall in the beating embryonic heart is essential and critical for experiment-based computational modelling and analysis of early-stage cardiac biomechanics. Although speckle variance optical coherence tomography (SV-OCT) relying on calculation of intensity variance over consecutively acquired frames is a powerful approach for segmentation of fluid flow from static tissue, application of this method in the beating embryonic heart remains challenging because moving structures generate SV signal indistinguishable from the blood. Here, we demonstrate a modified four-dimensional SV-OCT approach that effectively separates the blood flow from the dynamic heart wall in the beating mouse embryonic heart. The method takes advantage of the periodic motion of the cardiac wall and is based on calculation of the SV signal over the frames corresponding to the same phase of the heartbeat cycle. Through comparison with Doppler OCT imaging, we validate this speckle-based approach and show advantages in its insensitiveness to the flow direction and velocity as well as reduced influence from the heart wall movement. This approach has a potential in variety of applications relying on visualization and segmentation of blood flow in periodically moving structures, such as mechanical simulation studies and finite element modelling. Picture: Four-dimensional speckle variance OCT imaging shows the blood flow inside the beating heart of an E8.5 mouse embryo. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Comparison of methods for estimating ground-water recharge and base flow at a small watershed underlain by fractured bedrock in the Eastern United States

USGS Publications Warehouse

Risser, Dennis W.; Gburek, William J.; Folmar, Gordon J.

2005-01-01

This study by the U.S. Geological Survey (USGS), in cooperation with the Agricultural Research Service (ARS), U.S. Department of Agriculture, compared multiple methods for estimating ground-water recharge and base flow (as a proxy for recharge) at sites in east-central Pennsylvania underlain by fractured bedrock and representative of a humid-continental climate. This study was one of several within the USGS Ground-Water Resources Program designed to provide an improved understanding of methods for estimating recharge in the eastern United States. Recharge was estimated on a monthly and annual basis using four methods?(1) unsaturated-zone drainage collected in gravity lysimeters, (2) daily water balance, (3) water-table fluctuations in wells, and (4) equations of Rorabaugh. Base flow was estimated by streamflow-hydrograph separation using the computer programs PART and HYSEP. Estimates of recharge and base flow were compared for an 8-year period (1994-2001) coinciding with operation of the gravity lysimeters at an experimental recharge site (Masser Recharge Site) and a longer 34-year period (1968-2001), for which climate and streamflow data were available on a 2.8-square-mile watershed (WE-38 watershed). Estimates of mean-annual recharge at the Masser Recharge Site and WE-38 watershed for 1994-2001 ranged from 9.9 to 14.0 inches (24 to 33 percent of precipitation). Recharge, in inches, from the various methods was: unsaturated-zone drainage, 12.2; daily water balance, 12.3; Rorabaugh equations with PULSE, 10.2, or RORA, 14.0; and water-table fluctuations, 9.9. Mean-annual base flow from streamflow-hydrograph separation ranged from 9.0 to 11.6 inches (21-28 percent of precipitation). Base flow, in inches, from the various methods was: PART, 10.7; HYSEP Local Minimum, 9.0; HYSEP Sliding Interval, 11.5; and HYSEP Fixed Interval, 11.6. Estimating recharge from multiple methods is useful, but the inherent differences of the methods must be considered when comparing results. For example, although unsaturated-zone drainage from the gravity lysimeters provided the most direct measure of potential recharge, it does not incorporate spatial variability that is contained in watershed-wide estimates of net recharge from the Rorabaugh equations or base flow from streamflow-hydrograph separation. This study showed that water-level fluctuations, in particular, should be used with caution to estimate recharge in low-storage fractured-rock aquifers because of the variability of water-level response among wells and sensitivity of recharge to small errors in estimating specific yield. To bracket the largest range of plausible recharge, results from this study indicate that recharge derived from RORA should be compared with base flow from the Local-Minimum version of HYSEP.

Features of separating turbulent boundary layers

NASA Technical Reports Server (NTRS)

Nagabushana, K. A.; Agarwal, Naval K.; Simpson, Roger L.

1988-01-01

In the present study of two strong adverse pressure gradient flows, mean flow and turbulence characteristics are measured, together with frequency spectra, using hot-wire and laser anemometry. In these separating flows, reattachment occurs over a shorter distance than separation. It is noted that the outer flow variables form a unique set of scaling parameters for streamwise power spectra in adverse pressure gradient flows. The inner flow scaling of Perry et al. (1985) for streamwise spectra does not hold in the backflow region unless the value of the downstream-upstream intermittency in the flow is unity.

Vortex shedding within laminar separation bubbles forming over an airfoil

NASA Astrophysics Data System (ADS)

Kirk, Thomas M.; Yarusevych, Serhiy

2017-05-01

Vortex shedding within laminar separation bubbles forming over the suction side of a NACA 0018 airfoil is studied through a combination of high-speed flow visualization and boundary layer measurements. Wind tunnel experiments are performed at a chord-based Reynolds number of 100,000 and four angles of attack. The high-speed flow visualization is complemented by quantitative velocity and surface pressure measurements. The structures are shown to originate from the natural amplification of small-amplitude disturbances, and the shear layer roll-up is found to occur coherently across the span. However, significant cycle-to-cycle variations are observed in vortex characteristics, including shedding period and roll-up location. The formation of the roll-up vortices precedes the later stages of transition, during which these structures undergo significant deformations and breakdown to smaller scales. During this stage of flow development, vortex merging is also observed. The results provide new insight into the development of coherent structures in separation bubbles and their relation to the overall bubble dynamics and mean bubble topology.

Characteristics of Evaporator with a Lipuid-Vapor Separator

NASA Astrophysics Data System (ADS)

Ikeguchi, Masaki; Tanaka, Naoki; Yumikura, Tsuneo

Flow pattern of refrigerant in a heat exchanger tube changes depending on vapor quality, tube diameter, refrigerant flow rate and refrigerant properties. High flow rate causes mist flow where the quality is from 0.8 to 1.0. 1n this flow pattern, the liquid film detaches from the tube wall so that the heat flow is intervened. The heat transfer coefficient generally increases with the flow rate. But the pressure drop of refrigerant flow simultaneously increases and the region of the mist flow enlarges. In order to reduce the pressure drop and suppress the mist flow, we have developped a small liquid-vapor separator that removes the vapor from the evaporating refrigerant flow. This separator is equipped in the middle of the evaporator where the flow pattern is annular. The experiments to evaluate the effect of this separator were carried out and the following conclutions were obtained. (1) Average heat transfer coefficient increases by 30-60 %. (2) Pressure drop reduces by 20-30 %. (3) Cooling Capacity increases by 2-9 %.

Electrohydrodynamic effects in continuous flow electrophoresis

NASA Technical Reports Server (NTRS)

Rhodes, P. H.; Snyder, R. S.; Roberts, G. O.; Baygents, J. C.

1991-01-01

We demonstrate experimentally and theoretically the importance of electrohydrodynamic (EHD) flows in continuous-flow electrophoresis (CFE) separations. These flows are associated with variations in the conductivity or dielectric constant, and are quadratic in the field strength. They appear to be the main cause of extraneous and undesired flows in CFE which have degraded separation performance and have until now not been explained. We discuss the importance of EHD flows relative to other effects. We also describe possible techniques for reducing the associated degradation of CFE separations.

Investigation of Compressibility Effect for Aeropropulsive Shear Flows

NASA Technical Reports Server (NTRS)

Balasubramanyam, M. S.; Chen, C. P.

2005-01-01

Rocket Based Combined Cycle (RBCC) engines operate within a wide range of Mach numbers and altitudes. Fundamental fluid dynamic mechanisms involve complex choking, mass entrainment, stream mixing and wall interactions. The Propulsion Research Center at the University of Alabama in Huntsville is involved in an on- going experimental and numerical modeling study of non-axisymmetric ejector-based combined cycle propulsion systems. This paper attempts to address the modeling issues related to mixing, shear layer/wall interaction in a supersonic Strutjet/ejector flow field. Reynolds Averaged Navier-Stokes (RANS) solutions incorporating turbulence models are sought and compared to experimental measurements to characterize detailed flow dynamics. The effect of compressibility on fluids mixing and wall interactions were investigated using an existing CFD methodology. The compressibility correction to conventional incompressible two- equation models is found to be necessary for the supersonic mixing aspect of the ejector flows based on 2-D simulation results. 3-D strut-base flows involving flow separations were also investigated.

Very high pressure liquid chromatography using core-shell particles: quantitative analysis of fast gradient separations without post-run times.

PubMed

Stankovich, Joseph J; Gritti, Fabrice; Stevenson, Paul G; Beaver, Lois A; Guiochon, Georges

2014-01-17

Five methods for controlling the mobile phase flow rate for gradient elution analyses using very high pressure liquid chromatography (VHPLC) were tested to determine thermal stability of the column during rapid gradient separations. To obtain rapid separations, instruments are operated at high flow rates and high inlet pressure leading to uneven thermal effects across columns and additional time needed to restore thermal equilibrium between successive analyses. The purpose of this study is to investigate means to minimize thermal instability and obtain reliable results by measuring the reproducibility of the results of six replicate gradient separations of a nine component RPLC standard mixture under various experimental conditions with no post-run times. Gradient separations under different conditions were performed: constant flow rates, two sets of constant pressure operation, programmed flow constant pressure operation, and conditions which theoretically should yield a constant net heat loss at the column's wall. The results show that using constant flow rates, programmed flow constant pressures, and constant heat loss at the column's wall all provide reproducible separations. However, performing separations using a high constant pressure with programmed flow reduces the analysis time by 16% compared to constant flow rate methods. For the constant flow rate, programmed flow constant pressure, and constant wall heat experiments no equilibration time (post-run time) was required to obtain highly reproducible data. Copyright © 2013 Elsevier B.V. All rights reserved.

Comparative study of MacCormack and TVD MacCormack schemes for three-dimensional separation at wing/body junctions in supersonic flows

NASA Technical Reports Server (NTRS)

Lakshmanan, Balakrishnan; Tiwari, Surendra N.

1992-01-01

A robust, discontinuity-resolving TVD MacCormack scheme containing no dependent parameters requiring adjustment is presently used to investigate the 3D separation of wing/body junction flows at supersonic speeds. Many production codes employing MacCormack schemes can be adapted to use this method. A numerical simulation of laminar supersonic junction flow is found to yield improved separation location predictions, as well as the axial velocity profiles in the separated flow region.

Integrated turbomachine oxygen plant

DOEpatents

Anand, Ashok Kumar; DePuy, Richard Anthony; Muthaiah, Veerappan

2014-06-17

An integrated turbomachine oxygen plant includes a turbomachine and an air separation unit. One or more compressor pathways flow compressed air from a compressor through one or more of a combustor and a turbine expander to cool the combustor and/or the turbine expander. An air separation unit is operably connected to the one or more compressor pathways and is configured to separate the compressed air into oxygen and oxygen-depleted air. A method of air separation in an integrated turbomachine oxygen plant includes compressing a flow of air in a compressor of a turbomachine. The compressed flow of air is flowed through one or more of a combustor and a turbine expander of the turbomachine to cool the combustor and/or the turbine expander. The compressed flow of air is directed to an air separation unit and is separated into oxygen and oxygen-depleted air.

DSMC Simulations of Hypersonic Flows With Shock Interactions and Validation With Experiments

NASA Technical Reports Server (NTRS)

Moss, James N.; Bird, Graeme A.

2004-01-01

The capabilities of a relatively new direct simulation Monte Carlo (DSMC) code are examined for the problem of hypersonic laminar shock/shock and shock/boundary layer interactions, where boundary layer separation is an important feature of the flow. Flow about two model configurations is considered, where both configurations (a biconic and a hollow cylinder-flare) have recent published experimental measurements. The computations are made by using the DS2V code of Bird, a general two-dimensional/axisymmetric time accurate code that incorporates many of the advances in DSMC over the past decade. The current focus is on flows produced in ground-based facilities at Mach 12 and 16 test conditions with nitrogen as the test gas and the test models at zero incidence. Results presented highlight the sensitivity of the calculations to grid resolutions, sensitivity to physical modeling parameters, and comparison with experimental measurements. Information is provided concerning the flow structure and surface results for the extent of separation, heating, pressure, and skin friction.

DSMC Simulations of Hypersonic Flows With Shock Interactions and Validation With Experiments

NASA Technical Reports Server (NTRS)

Moss, James N.; Bird, Graeme A.

2004-01-01

The capabilities of a relatively new direct simulation Monte Carlo (DSMC) code are examined for the problem of hypersonic laminar shock/shock and shock/boundary layer interactions, where boundary layer separation is an important feature of the flow. Flow about two model configurations is considered, where both configurations (a biconic and a hollow cylinder-flare) have recent published experimental measurements. The computations are made by using the DS2V code of Bird, a general two-dimensional/axisymmetric time accurate code that incorporates many of the advances in DSMC over the past decade. The current focus is on flows produced in ground-based facilities at Mach 12 and 16 test conditions with nitrogen as the test gas and the test models at zero incidence. Results presented highlight the sensitivity of the calculations to grid resolution, sensitivity to physical modeling parameters, and comparison with experimental measurements. Information is provided concerning the flow structure and surface results for the extent of separation, heating, pressure, and skin friction.

Computational aspects of unsteady flows

NASA Technical Reports Server (NTRS)

Cebeci, T.; Carr, L. W.; Khattab, A. A.; Schimke, S. M.

1985-01-01

The calculation of unsteady flows and the development of numerical methods for solving unsteady boundary layer equations and their application to the flows around important configurations such as oscillating airfoils are presented. A brief review of recent work is provided with emphasis on the need for numerical methods which can overcome possible problems associated with flow reversal and separation. The zig-zag and characteristic box schemes are described in this context, and when embodied in a method which permits interaction between solutions of inviscid and viscous equations, the characteristic box scheme is shown to avoid the singularity associated with boundary layer equations and prescribed pressure gradient. Calculations were performed for a cylinder started impulsively from rest and oscillating airfoils. The results are presented and discussed. It is conlcuded that turbulence models based on an algebraic specification of eddy viscosity can be adequate, that location of translation is important to the calculation of the location of flow separation and, therefore, to the overall lift of an oscillating airfoil.

Inflow/outflow boundary conditions for particle-based blood flow simulations: Application to arterial bifurcations and trees

DOE PAGES

Lykov, Kirill; Li, Xuejin; Lei, Huan; ...

2015-08-28

When blood flows through a bifurcation, red blood cells (RBCs) travel into side branches at different hematocrit levels, and it is even possible that all RBCs enter into one branch only, leading to a complete separation of plasma and R- BCs. To quantify this phenomenon via particle-based mesoscopic simulations, we developed a general framework for open boundary conditions in multiphase flows that is effective even for high hematocrit levels. The inflow at the inlet is duplicated from a fully developed flow generated in a pilot simulation with periodic boundary conditions. The outflow is controlled by adaptive forces to maintain themore » flow rate and velocity gradient at fixed values, while the particles leaving the arteriole at the outlet are removed from the system. Upon valida- tion of this approach, we performed systematic 3D simulations to study plasma skimming in arterioles of diameters 20 to 32 microns. For a flow rate ratio 6:1 at the branches, we observed the \\all-or-nothing" phenomenon with plasma only entering the low flow rate branch. We then simulated blood-plasma separation in arteriolar bifurcations with different bifurcation angles and same diameter of the daughter branches. Our simulations predict a significant increase in RBC flux through the main daughter branch as the bifurcation angle is increased. Lastly, we demonstrated the new methodology for simulating blood flow in ves- sels with multiple inlets and outlets, constructed using an angiogenesis model.« less

Inflow/outflow boundary conditions for particle-based blood flow simulations: Application to arterial bifurcations and trees

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lykov, Kirill; Li, Xuejin; Lei, Huan

When blood flows through a bifurcation, red blood cells (RBCs) travel into side branches at different hematocrit levels, and it is even possible that all RBCs enter into one branch only, leading to a complete separation of plasma and R- BCs. To quantify this phenomenon via particle-based mesoscopic simulations, we developed a general framework for open boundary conditions in multiphase flows that is effective even for high hematocrit levels. The inflow at the inlet is duplicated from a fully developed flow generated in a pilot simulation with periodic boundary conditions. The outflow is controlled by adaptive forces to maintain themore » flow rate and velocity gradient at fixed values, while the particles leaving the arteriole at the outlet are removed from the system. Upon valida- tion of this approach, we performed systematic 3D simulations to study plasma skimming in arterioles of diameters 20 to 32 microns. For a flow rate ratio 6:1 at the branches, we observed the \\all-or-nothing" phenomenon with plasma only entering the low flow rate branch. We then simulated blood-plasma separation in arteriolar bifurcations with different bifurcation angles and same diameter of the daughter branches. Our simulations predict a significant increase in RBC flux through the main daughter branch as the bifurcation angle is increased. Lastly, we demonstrated the new methodology for simulating blood flow in ves- sels with multiple inlets and outlets, constructed using an angiogenesis model.« less

Inflow/Outflow Boundary Conditions for Particle-Based Blood Flow Simulations: Application to Arterial Bifurcations and Trees.

PubMed

Lykov, Kirill; Li, Xuejin; Lei, Huan; Pivkin, Igor V; Karniadakis, George Em

2015-08-01

When blood flows through a bifurcation, red blood cells (RBCs) travel into side branches at different hematocrit levels, and it is even possible that all RBCs enter into one branch only, leading to a complete separation of plasma and RBCs. To quantify this phenomenon via particle-based mesoscopic simulations, we developed a general framework for open boundary conditions in multiphase flows that is effective even for high hematocrit levels. The inflow at the inlet is duplicated from a fully developed flow generated in a pilot simulation with periodic boundary conditions. The outflow is controlled by adaptive forces to maintain the flow rate and velocity gradient at fixed values, while the particles leaving the arteriole at the outlet are removed from the system. Upon validation of this approach, we performed systematic 3D simulations to study plasma skimming in arterioles of diameters 20 to 32 microns. For a flow rate ratio 6:1 at the branches, we observed the "all-or-nothing" phenomenon with plasma only entering the low flow rate branch. We then simulated blood-plasma separation in arteriolar bifurcations with different bifurcation angles and same diameter of the daughter branches. Our simulations predict a significant increase in RBC flux through the main daughter branch as the bifurcation angle is increased. Finally, we demonstrated the effectiveness of the new methodology in simulations of blood flow in vessels with multiple inlets and outlets, constructed using an angiogenesis model.

Comparing model-based and model-free analysis methods for QUASAR arterial spin labeling perfusion quantification.

PubMed

Chappell, Michael A; Woolrich, Mark W; Petersen, Esben T; Golay, Xavier; Payne, Stephen J

2013-05-01

Amongst the various implementations of arterial spin labeling MRI methods for quantifying cerebral perfusion, the QUASAR method is unique. By using a combination of labeling with and without flow suppression gradients, the QUASAR method offers the separation of macrovascular and tissue signals. This permits local arterial input functions to be defined and "model-free" analysis, using numerical deconvolution, to be used. However, it remains unclear whether arterial spin labeling data are best treated using model-free or model-based analysis. This work provides a critical comparison of these two approaches for QUASAR arterial spin labeling in the healthy brain. An existing two-component (arterial and tissue) model was extended to the mixed flow suppression scheme of QUASAR to provide an optimal model-based analysis. The model-based analysis was extended to incorporate dispersion of the labeled bolus, generally regarded as the major source of discrepancy between the two analysis approaches. Model-free and model-based analyses were compared for perfusion quantification including absolute measurements, uncertainty estimation, and spatial variation in cerebral blood flow estimates. Major sources of discrepancies between model-free and model-based analysis were attributed to the effects of dispersion and the degree to which the two methods can separate macrovascular and tissue signal. Copyright © 2012 Wiley Periodicals, Inc.

Visualization of Flow Separation Around an Atmospheric Entry Capsule at Low-Subsonic Mach Number Using Background-Oriented Schlieren (BOS)

NASA Technical Reports Server (NTRS)

Mizukaki, Toshiharu; Borg, Stephen E.; Danehy, Paul M.; Murman, Scott M.

2014-01-01

This paper presents the results of visualization of separated flow around a generic entry capsule that resembles the Apollo Command Module (CM) and the Orion Multi-Purpose Crew Vehicle (MPCV). The model was tested at flow speeds up to Mach 0.4 at a single angle of attack of 28 degrees. For manned spacecraft using capsule-shaped vehicles, certain flight operations such as emergency abort maneuvers soon after launch and flight just prior to parachute deployment during the final stages of entry, the command module may fly at low Mach number. Under these flow conditions, the separated flow generated from the heat-shield surface on both windward and leeward sides of the capsule dominates the wake flow downstream of the capsule. In this paper, flow visualization of the separated flow was conducted using the background-oriented schlieren (BOS) method, which has the capability of visualizing significantly separated wake flows without the particle seeding required by other techniques. Experimental results herein show that BOS has detection capability of density changes on the order of 10(sup-5).

A study of the laminar separation bubble on an airfoil at low Reynolds numbers using flow visualization techniques

NASA Technical Reports Server (NTRS)

Schmidt, Gordon S.; Mueller, Thomas J.

1987-01-01

The use of flow visualization to study separation bubbles is evaluated. The wind tunnel, two NACA 66(3)-018 airfoil models, and kerosene vapor, titanium tetrachloride, and surface flow visualizations techniques are described. The application of the three visualization techniques to the two airfoil models reveals that the smoke and vapor techniques provide data on the location of laminar separation and the onset of transition, and the surface method produces information about the location of turbulent boundary layer separation. The data obtained with the three flow visualization techniques are compared to pressure distribution data and good correlation is detected. It is noted that flow visualization is an effective technique for examining separation bubbles.

Flow control of micro-ramps on supersonic forward-facing step flow

NASA Astrophysics Data System (ADS)

Qing-Hu, Zhang; Tao, Zhu; Shihe, Yi; Anping, Wu

2016-05-01

The effects of the micro-ramps on supersonic turbulent flow over a forward-facing step (FFS) was experimentally investigated in a supersonic low-noise wind tunnel at Mach number 3 using nano-tracer planar laser scattering (NPLS) and particle image velocimetry (PIV) techniques. High spatiotemporal resolution images and velocity fields of supersonic flow over the testing model were captured. The fine structures and their spatial evolutionary characteristics without and with the micro-ramps were revealed and compared. The large-scale structures generated by the micro-ramps can survive the downstream FFS flowfield. The micro-ramps control on the flow separation and the separation shock unsteadiness was investigated by PIV results. With the micro-ramps, the reduction in the range of the reversal flow zone in streamwise direction is 50% and the turbulence intensity is also reduced. Moreover, the reduction in the average separated region and in separation shock unsteadiness are 47% and 26%, respectively. The results indicate that the micro-ramps are effective in reducing the flow separation and the separation shock unsteadiness. Project supported by the National Natural Science Foundation of China (Grant Nos. 11172326 and 11502280).

Stripe-patterned thermo-responsive cell culture dish for cell separation without cell labeling.

PubMed

Kumashiro, Yoshikazu; Ishihara, Jun; Umemoto, Terumasa; Itoga, Kazuyoshi; Kobayashi, Jun; Shimizu, Tatsuya; Yamato, Masayuki; Okano, Teruo

2015-02-11

A stripe-patterned thermo-responsive surface is prepared to enable cell separation without labeling. The thermo-responsive surface containing a 3 μm striped pattern exhibits various cell adhesion and detachment properties. A mixture of three cell types is separated on the patterned surface based on their distinct cell-adhesion properties, and the composition of the cells is analyzed by flow cytometry. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Computation of viscous flows over airfoils, including separation, with a coupling approach

NASA Technical Reports Server (NTRS)

Leballeur, J. C.

1983-01-01

Viscous incompressible flows over single or multiple airfoils, with or without separation, were computed using an inviscid flow calculation, with modified boundary conditions, and by a method providing calculation and coupling for boundary layers and wakes, within conditions of strong viscous interaction. The inviscid flow is calculated with a method of singularities, the numerics of which were improved by using both source and vortex distributions over profiles, associated with regularity conditions for the fictitious flows inside of the airfoils. The viscous calculation estimates the difference between viscous flow and inviscid interacting flow, with a direct or inverse integral method, laminar or turbulent, with or without reverse flow. The numerical method for coupling determines iteratively the boundary conditions for the inviscid flow. For attached viscous layers regions, an underrelaxation is locally calculated to insure stability. For separated or separating regions, a special semi-inverse algorithm is used. Comparisons with experiments are presented.

Flow separation in a computational oscillating vocal fold model

NASA Astrophysics Data System (ADS)

Alipour, Fariborz; Scherer, Ronald C.

2004-09-01

A finite-volume computational model that solves the time-dependent glottal airflow within a forced-oscillation model of the glottis was employed to study glottal flow separation. Tracheal input velocity was independently controlled with a sinusoidally varying parabolic velocity profile. Control parameters included flow rate (Reynolds number), oscillation frequency and amplitude of the vocal folds, and the phase difference between the superior and inferior glottal margins. Results for static divergent glottal shapes suggest that velocity increase caused glottal separation to move downstream, but reduction in velocity increase and velocity decrease moved the separation upstream. At the fixed frequency, an increase of amplitude of the glottal walls moved the separation further downstream during glottal closing. Increase of Reynolds number caused the flow separation to move upstream in the glottis. The flow separation cross-sectional ratio ranged from approximately 1.1 to 1.9 (average of 1.47) for the divergent shapes. Results suggest that there may be a strong interaction of rate of change of airflow, inertia, and wall movement. Flow separation appeared to be ``delayed'' during the vibratory cycle, leading to movement of the separation point upstream of the glottal end only after a significant divergent angle was reached, and to persist upstream into the convergent phase of the cycle.

Experimental Study of Unsteady Separation in a Laminar Boundary Layer

NASA Astrophysics Data System (ADS)

Bonacci, Andrew; Lang, Amy; Wahidi, Redha; Santos, Leo

2016-11-01

Separation, caused by an adverse pressure gradient, can be a major problem to aircraft. Reversing flow occurs in separated regions and an investigation of how this backflow forms is of interest due to the fact that this could be used as a means of initiating flow control. Specifically, backflow can bristle shark scales which may be linked to a passive, flow actuated separation control mechanism. An experiment was conducted in a water tunnel to replicate separation, with a focus on the reversing flow development near the wall within a laminar boundary layer. Using a rotating cylinder, an adverse pressure gradient was induced creating a separated region over a flat plate. In this experiment the boundary layer grows to sizes great enough that the scale of the flow is increased, making it more measurable to DPIV. In the future, this research can be utilized to better understand flow control mechanisms such as those enabled by shark skin. Funding from Army Research Office and NSF REU site Grant EEC 1358991 is greatly appreciated.

Measurement of flow separation in a human vocal folds model

NASA Astrophysics Data System (ADS)

Šidlof, Petr; Doaré, Olivier; Cadot, Olivier; Chaigne, Antoine

2011-07-01

The paper provides experimental data on flow separation from a model of the human vocal folds. Data were measured on a four times scaled physical model, where one vocal fold was fixed and the other oscillated due to fluid-structure interaction. The vocal folds were fabricated from silicone rubber and placed on elastic support in the wall of a transparent wind tunnel. A PIV system was used to visualize the flow fields immediately downstream of the glottis and to measure the velocity fields. From the visualizations, the position of the flow separation point was evaluated using a semiautomatic procedure and plotted for different airflow velocities. The separation point position was quantified relative to the orifice width separately for the left and right vocal folds to account for flow asymmetry. The results indicate that the flow separation point remains close to the narrowest cross-section during most of the vocal fold vibration cycle, but moves significantly further downstream shortly prior to and after glottal closure.

[Effects of carrier liquid and flow rate on the separation in gravitational field-flow fractionation].

PubMed

Guo, Shuang; Zhu, Chenqi; Gao-Yang, Yaya; Qiu, Bailing; Wu, Di; Liang, Qihui; He, Jiayuan; Han, Nanyin

2016-02-01

Gravitational field-flow fractionation is the simplest field-flow fractionation technique in terms of principle and operation. The earth' s gravity is its external field. Different sized particles are injected into a thin channel and carried by carrier fluid. The different velocities of the carrier liquid in different places results in a size-based separation. A gravitational field-flow fractionation (GrFFF) instrument was designed and constructed. Two kinds of polystyrene (PS) particles with different sizes (20 µm and 6 µm) were chosen as model particles. In this work, the separation of the sample was achieved by changing the concentration of NaN3, the percentage of mixed surfactant in the carrier liquid and the flow rate of carrier liquid. Six levels were set for each factor. The effects of these three factors on the retention ratio (R) and plate height (H) of the PS particles were investigated. It was found that R increased and H decreased with increasing particle size. On the other hand, the R and H increased with increasing flow rate. The R and H also increased with increasing NaN3 concentration. The reason was that the electrostatic repulsive force between the particles and the glass channel wall increased. The force allowed the samples approach closer to the channel wall. The results showed that the resolution and retention time can be improved by adjusting the experimental conditions. These results can provide important values to the further applications of GrFFF technique.

Unsteady flow through in-vitro models of the glottis

NASA Astrophysics Data System (ADS)

Hofmans, G. C. J.; Groot, G.; Ranucci, M.; Graziani, G.; Hirschberg, A.

2003-03-01

The unsteady two-dimensional flow through fixed rigid in vitro models of the glottis is studied in some detail to validate a more accurate model based on the prediction of boundary-layer separation. The study is restricted to the flow phenomena occurring within the glottis and does not include effects of vocal-fold movement on the flow. Pressure measurements have been carried out for a transient flow through a rigid scale model of the glottis. The rigid model with a fixed geometry driven by an unsteady pressure is used in order to achieve a high accuracy in the specification of the geometry of the glottis. The experimental study is focused on flow phenomena as they might occur in the glottis, such as the asymmetry of the flow due to the Coanda effect and the transition to turbulent flow. It was found that both effects need a relatively long time to establish themselves and are therefore unlikely to occur during the production of normal voiced speech when the glottis closes completely during part of the oscillation cycle. It is shown that when the flow is still laminar and symmetric the prediction of the boundary-layer model and the measurement of the pressure drop from the throat of the glottis to the exit of the glottis agree within 40%. Results of the boundary-layer model are compared with a two-dimensional vortex-blob method for viscous flow. The difference between the results of the simpiflied boundary-layer model and the experimental results is explained by an additional pressure difference between the separation point and the far field within the jet downstream of the separation point. The influence of the movement of the vocal folds on our conclusions is still unclear.

Experiments on an unsteady, three-dimensional separation

NASA Technical Reports Server (NTRS)

Henk, R. W.; Reynolds, W. C.; Reed, H. L.

1992-01-01

Unsteady, three-dimensional flow separation occurs in a variety of technical situations including turbomachinery and low-speed aircraft. An experimental program at Stanford in unsteady, three-dimensional, pressure-driven laminar separation has investigated the structure and time-scaling of these flows; of particular interest is the development, washout, and control of flow separation. Results reveal that a two-dimensional, laminar boundary layer passes through several stages on its way to a quasi-steady three-dimensional separation. The quasi-steady state of the separation embodies a complex, unsteady, vortical structure.

Numerical simulation of transitional flow on a wind turbine airfoil with RANS-based transition model

NASA Astrophysics Data System (ADS)

Zhang, Ye; Sun, Zhengzhong; van Zuijlen, Alexander; van Bussel, Gerard

2017-09-01

This paper presents a numerical investigation of transitional flow on the wind turbine airfoil DU91-W2-250 with chord-based Reynolds number Rec = 1.0 × 106. The Reynolds-averaged Navier-Stokes based transition model using laminar kinetic energy concept, namely the k - kL - ω model, is employed to resolve the boundary layer transition. Some ambiguities for this model are discussed and it is further implemented into OpenFOAM-2.1.1. The k - kL - ω model is first validated through the chosen wind turbine airfoil at the angle of attack (AoA) of 6.24° against wind tunnel measurement, where lift and drag coefficients, surface pressure distribution and transition location are compared. In order to reveal the transitional flow on the airfoil, the mean boundary layer profiles in three zones, namely the laminar, transitional and fully turbulent regimes, are investigated. Observation of flow at the transition location identifies the laminar separation bubble. The AoA effect on boundary layer transition over wind turbine airfoil is also studied. Increasing the AoA from -3° to 10°, the laminar separation bubble moves upstream and reduces in size, which is in close agreement with wind tunnel measurement.

Investigation of Convection and Pressure Treatment with Splitting Techniques

NASA Technical Reports Server (NTRS)

Thakur, Siddharth; Shyy, Wei; Liou, Meng-Sing

1995-01-01

Treatment of convective and pressure fluxes in the Euler and Navier-Stokes equations using splitting formulas for convective velocity and pressure is investigated. Two schemes - controlled variation scheme (CVS) and advection upstream splitting method (AUSM) - are explored for their accuracy in resolving sharp gradients in flows involving moving or reflecting shock waves as well as a one-dimensional combusting flow with a strong heat release source term. For two-dimensional compressible flow computations, these two schemes are implemented in one of the pressure-based algorithms, whose very basis is the separate treatment of convective and pressure fluxes. For the convective fluxes in the momentum equations as well as the estimation of mass fluxes in the pressure correction equation (which is derived from the momentum and continuity equations) of the present algorithm, both first- and second-order (with minmod limiter) flux estimations are employed. Some issues resulting from the conventional use in pressure-based methods of a staggered grid, for the location of velocity components and pressure, are also addressed. Using the second-order fluxes, both CVS and AUSM type schemes exhibit sharp resolution. Overall, the combination of upwinding and splitting for the convective and pressure fluxes separately exhibits robust performance for a variety of flows and is particularly amenable for adoption in pressure-based methods.

Investigation of jet exhaust and local Mach number effects on the Solid Rocket Booster (SRB) base pressures (fa19)

NASA Technical Reports Server (NTRS)

Love, D. A.

1978-01-01

Two single nozzles with flare angles of 10 and 20 degrees were tested at Mach numbers of 0.5, 0.9, 1.2, 1.46, 1.96 and 3.48 in the presence of gaseous plumes. An attempt was made to determine the local Mach number above the flare by utilizing a pitot probe. This objective was only partially satisfied because the 20 degree flare separated the flow ahead of the flare for Mach numbers of 0.5 to 1.96. An accurate local Mach number could not be determined because of the separated flow. To meet the objective of a data base as a function of freestream Mach number, model surface and base pressures were obtained in the presence of gaseous plumes for a matrix of chamber pressures and temperatures at Mach numbers of 0.5, 0.9, 1.2, 1.46, 1.96 and 3.48.

[A method for the analysis of overlapped peaks in the high performance liquid chromatogram based on spectrum analysis].

PubMed

Liu, Bao; Fan, Xiaoming; Huo, Shengnan; Zhou, Lili; Wang, Jun; Zhang, Hui; Hu, Mei; Zhu, Jianhua

2011-12-01

A method was established to analyse the overlapped chromatographic peaks based on the chromatographic-spectra data detected by the diode-array ultraviolet detector. In the method, the three-dimensional data were de-noised and normalized firstly; secondly the differences and clustering analysis of the spectra at different time points were calculated; then the purity of the whole chromatographic peak were analysed and the region were sought out in which the spectra of different time points were stable. The feature spectra were extracted from the spectrum-stable region as the basic foundation. The nonnegative least-square method was chosen to separate the overlapped peaks and get the flow curve which was based on the feature spectrum. The three-dimensional divided chromatographic-spectrum peak could be gained by the matrix operations of the feature spectra with the flow curve. The results displayed that this method could separate the overlapped peaks.

Sheathless interface to match flow rate of capillary electrophoresis with electrospray mass spectrometry using regular-sized capillary.

PubMed

Yin, Yue; Li, Gongyu; Guan, Yafeng; Huang, Guangming

2016-08-01

The flow rate match has been a great challenge when coupling capillary electrophoresis (CE) with electrospray ionization mass spectrometry (ESI-MS). Conventional CE-ESI-MS interfaces used liquid sheath flow, narrowed capillary or additional pressure to meet this requirement; sacrifice of either capillary inner diameter (i.d.) or separation efficiency is often inevitable. Thus, a regular-sized capillary-based sheathless interface would be attractive for flow rate match in CE-MS. The regular-sized capillary-based CE-MS interface was achieved by coupling CE with induced electrospray ionization (iESI) which was stimulated by the fact that the iESI could both achieve flow rate down to 0.2 μL/min and retain ionization efficiency. The CE-iESI-MS interface was completed with an intact separation capillary, outside the outlet end of which a metal electrode was attached for the application of alternating current (ac) high voltage (HV). The feasibility of this CE-iESI-MS interface was demonstrated through the stable total ion chromatograms obtained by continuous CE infusion of tripropylamine with regular-sized capillaries. Tripropylamine and atenolol were separated and detected successfully in phosphate buffer solution (PBS) by CE-iESI-MS using a 50 or 75 μm i.d. capillary. Furthermore, this new interface showed a better signal-to-noise (S/N) of 3 to 7 times enhancement compared with another sheathless CE-ESI-MS interface that using one high voltage for both separation and electrospray when analyzing the mixture of tripropylamine and proline in NH4 OAc buffer. In addition, the reproducibility of this interface gave satisfactory results with relative standard deviation (RSD) in retention time in the range between 1% and 3%. The novel sheathless CE-MS interface introduced here could match conventional electroosmotic flow (EOF) with electrospray which could also preserve the separation efficiency and sensitivity of CE-MS. This newly developed CE-iESI-MS interface was also demonstrated to be effective for different buffers, PBS and NH4 OAc, without any additives such as methanol and acetic acid. Hence, we believe that this sheathless CE-MS interface could be operated with other nonvolatile and volatile buffers. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

A computational study of incipient leading-edge separation on a 65-deg delta wing at M = 1.60

NASA Technical Reports Server (NTRS)

Mcmillin, S. Naomi; Pittman, James L.; Thomas, James L.

1990-01-01

A computational study on a 65-deg delta wing at a freestream Mach number of 1.60 has been conducted by obtaining conical Reynolds-averaged Navier-Stokes solutions on a parametric series of geometries which varied in leading-edge radius and/or circular-arc camber. The computational results showed that increasing leading-edge radius or camber can delay the onset of leading-edge separation on the leeside of a delta wing at a specific angle of attack. Reynolds number was varied from 1 x 10 to the 6th to 5 x 10 to the 6th for a turbulent boundary-layer and was shown to have a minor effect on the effectiveness of leading-edge radius and/or camber in delaying the onset of leading-edge separation. Both laminar and turbulent boundary-layer models were investigated at a Reynolds number of 1 x 10 to the 6th, and the predicted flow pattern was found to change from attached flow for the turbulent boundary-layer model to separated flow for the laminar boundary-layer model. Based upon these results, three wind-tunnel models have been designed to be tested in the Langley Unitary Plan Wind Tunnel.

Computation of transonic separated wing flows using an Euler/Navier-Stokes zonal approach

NASA Technical Reports Server (NTRS)

Kaynak, Uenver; Holst, Terry L.; Cantwell, Brian J.

1986-01-01

A computer program called Transonic Navier Stokes (TNS) has been developed which solves the Euler/Navier-Stokes equations around wings using a zonal grid approach. In the present zonal scheme, the physical domain of interest is divided into several subdomains called zones and the governing equations are solved interactively. The advantages of the Zonal Grid approach are as follows: (1) the grid for any subdomain can be generated easily; (2) grids can be, in a sense, adapted to the solution; (3) different equation sets can be used in different zones; and, (4) this approach allows for a convenient data base organization scheme. Using this code, separated flows on a NACA 0012 section wing and on the NASA Ames WING C have been computed. First, the effects of turbulence and artificial dissipation models incorporated into the code are assessed by comparing the TNS results with other CFD codes and experiments. Then a series of flow cases is described where data are available. The computed results, including cases with shock-induced separation, are in good agreement with experimental data. Finally, some futuristic cases are presented to demonstrate the abilities of the code for massively separated cases which do not have experimental data.

Reversing flow causes passive shark scale actuation in a separating turbulent boundary layer

NASA Astrophysics Data System (ADS)

Lang, Amy; Gemmell, Bradford; Motta, Phil; Habegger, Laura; Du Clos, Kevin; Devey, Sean; Stanley, Caleb; Santos, Leo

2017-11-01

Control of flow separation by shortfin mako skin in experiments has been demonstrated, but the mechanism is still poorly understood yet must be to some extent Re independent. The hypothesized mechanisms inherent in the shark skin for controlling flow separation are: (1) the scales, which are capable of being bristled only by reversing flow, inhibit flow reversal events from further development into larger-scale separation and (2) the cavities formed when scales bristle induces mixing of high momentum flow towards the wall thus energizing the flow close to the surface. Two studies were carried out to measure passive scale actuation caused by reversing flow. A small flow channel induced an unsteady, wake flow over the scales prompting reversing flow events and scale actuation. To resolve the flow and scale movements simultaneously we used specialized optics at high magnification (1 mm field of view) at 50,000 fps. In another study, 3D printed models of shark scales, or microflaps (bristling capability up to 50 degrees), were set into a flat plate. Using a tripped, turbulent boundary layer grown over the long flat plate and a localized adverse pressure gradient, a separation bubble was generated within which the microflaps were placed. Passive flow actuation of both shark scales and microflaps by reversing flow was observed. Funding from Army Research Office and NSF REU site Grant.

Flow field investigation in a bulb turbine diffuser

NASA Astrophysics Data System (ADS)

Pereira, M.; Duquesne, P.; Aeschlimann, V.; Deschênes, C.

2017-04-01

An important drop in turbine performances has been measured in a bulb turbine model operated at overload. Previous investigations have correlated the performance drop with diffuser losses, and particularly to the flow separation zone at the diffuser wall. The flow has been investigated in the transition part of the diffuser using two LDV measurement sections. The transition part is a diffuser section that transforms from a circular to a rectangular section. The two measurement sections are at the inlet and outlet of the diffuser transition part. The turbine has been operated at three operating points, which are representative of different flow patterns at the diffuser exit at overload. In addition to the average velocity field, the analysis is conducted based on a backflow occurrence function and on the swirl level. Results reveal a counter-rotating zone in the diffuser, which intensifies with the guide vanes opening. The guide vanes opening induces a modification of the flow phenomena: from a central backflow recirculation zone at the lowest flowrate to a backflow zone induced by flow separation at the wall at the highest flowrate.

Use of streamflow data to estimate base flowground-water recharge for Wisconsin

USGS Publications Warehouse

Gebert, W.A.; Radloff, M.J.; Considine, E.J.; Kennedy, J.L.

2007-01-01

The average annual base flow/recharge was determined for streamflow-gaging stations throughout Wisconsin by base-flow separation. A map of the State was prepared that shows the average annual base flow for the period 1970-99 for watersheds at 118 gaging stations. Trend analysis was performed on 22 of the 118 streamflow-gaging stations that had long-term records, unregulated flow, and provided aerial coverage of the State. The analysis found that a statistically significant increasing trend was occurring for watersheds where the primary land use was agriculture. Most gaging stations where the land cover was forest had no significant trend. A method to estimate the average annual base flow at ungaged sites was developed by multiple-regression analysis using basin characteristics. The equation with the lowest standard error of estimate, 9.5%, has drainage area, soil infiltration and base flow factor as independent variables. To determine the average annual base flow for smaller watersheds, estimates were made at low-flow partial-record stations in 3 of the 12 major river basins in Wisconsin. Regression equations were developed for each of the three major river basins using basin characteristics. Drainage area, soil infiltration, basin storage and base-flow factor were the independent variables in the regression equations with the lowest standard error of estimate. The standard error of estimate ranged from 17% to 52% for the three river basins. ?? 2007 American Water Resources Association.

The Dynamics of Controlled Flow Separation within a Diverter Duct Diffuser

NASA Astrophysics Data System (ADS)

Peterson, C. J.; Vukasinovic, B.; Glezer, A.

2016-11-01

The evolution and receptivity to fluidic actuation of the flow separation within a rectangular, constant-width, diffuser that is branched off of a primary channel is investigated experimentally at speeds up to M = 0.4. The coupling between the diffuser's adverse pressure gradient and the internal separation that constricts nearly half of the flow passage through the duct is controlled using a spanwise array of fluidic actuators on the surface upstream of the diffuser's inlet plane. The dynamics of the separating surface vorticity layer in the absence and presence of actuation are investigated using high-speed particle image velocimetry combined with surface pressure measurements and total pressure distributions at the primary channel's exit plane. It is shown that the actuation significantly alters the incipient dynamics of the separating vorticity layer as the characteristic cross stream scales of the boundary layer upstream of separation and of the ensuing vorticity concentrations within the separated flow increase progressively with actuation level. It is argued that the dissipative (high frequency) actuation alters the balance between large- and small-scale motions near separation by intensifying the large-scale motions and limiting the small-scale dynamics. Controlling separation within the diffuser duct also has a profound effect on the global flow. In the presence of actuation, the mass flow rate in the primary duct increases 10% while the fraction of the diverted mass flow rate in the diffuser increases by more than 45% at 0.7% actuation mass fraction. Supported by the Boeing Company.

Hydrograph separation for karst watersheds using a two-domain rainfall-discharge model

USGS Publications Warehouse

Long, Andrew J.

2009-01-01

Highly parameterized, physically based models may be no more effective at simulating the relations between rainfall and outflow from karst watersheds than are simpler models. Here an antecedent rainfall and convolution model was used to separate a karst watershed hydrograph into two outflow components: one originating from focused recharge in conduits and one originating from slow flow in a porous annex system. In convolution, parameters of a complex system are lumped together in the impulse-response function (IRF), which describes the response of the system to an impulse of effective precipitation. Two parametric functions in superposition approximate the two-domain IRF. The outflow hydrograph can be separated into flow components by forward modeling with isolated IRF components, which provides an objective criterion for separation. As an example, the model was applied to a karst watershed in the Madison aquifer, South Dakota, USA. Simulation results indicate that this watershed is characterized by a flashy response to storms, with a peak response time of 1 day, but that 89% of the flow results from the slow-flow domain, with a peak response time of more than 1 year. This long response time may be the result of perched areas that store water above the main water table. Simulation results indicated that some aspects of the system are stationary but that nonlinearities also exist.

A theoretical method for the analysis and design of axisymmetric bodies. [flow distribution and incompressible fluids

NASA Technical Reports Server (NTRS)

Beatty, T. D.

1975-01-01

A theoretical method is presented for the computation of the flow field about an axisymmetric body operating in a viscous, incompressible fluid. A potential flow method was used to determine the inviscid flow field and to yield the boundary conditions for the boundary layer solutions. Boundary layer effects in the forces of displacement thickness and empirically modeled separation streamlines are accounted for in subsequent potential flow solutions. This procedure is repeated until the solutions converge. An empirical method was used to determine base drag allowing configuration drag to be computed.

A New Modular Approach for Tightly Coupled Fluid/Structure Analysis

NASA Technical Reports Server (NTRS)

Guruswamy, Guru

2003-01-01

Static aeroelastic computations are made using a C++ executive suitable for closely coupled fluid/structure interaction studies. The fluid flow is modeled using the Euler/Navier Stokes equations and the structure is modeled using finite elements. FORTRAN based fluids and structures codes are integrated under C++ environment. The flow and structural solvers are treated as separate object files. The data flow between fluids and structures is accomplished using I/O. Results are demonstrated for transonic flow over partially flexible surface that is important for aerospace vehicles. Use of this development to accurately predict flow induced structural failure will be demonstrated.

Experimental investigations on airfoils with different geometries in the domain of high angles of attack-flow separation

NASA Technical Reports Server (NTRS)

Keil, J.

1985-01-01

Wind tunnel tests were conducted on airfoil models in order to study the flow separation phenomena occurring for high angles of attack. Pressure distribution on wings of different geometries were measured. Results show that for three-dimensional airfoils layout and span lift play a role. Separation effects on airfoils with moderate extension are three-dimensional. The flow domains separated from the air foil must be treated three-dimensionally. The rolling-up of separated vortex layers increases with angle in intensity and induction effect and shows strong nonlinearities. Boundary layer material moves perpendicularly to the flow direction due to the pressure gradients at the airfoil; this has a stabilizing effect. The separation starts earlier with increasing pointed profiles.

The Effect of Micro-ramps on Supersonic Flow over a Forward-Facing Step

NASA Astrophysics Data System (ADS)

Zhang, Qing-Hu; Yi, Shi-He; Zhu, Yang-Zhu; Chen, Zhi; Wu, Yu

2013-04-01

The effect of micro-ramp control on fully developed turbulent flow over a forward-facing step (FFS) is investigated in a supersonic low-noise wind tunnel at Mach number 3 using nano-tracer planar laser scattering (NPLS) and supersonic particle image velocimetry (PIV) techniques. High spatiotemporal resolution images and the average velocity profiles of supersonic flow over the FFS with and without the control of the micro-ramps are captured. The fine structures of both cases, including the coherent structures of fully developed boundary layer and the large-scale hairpin-like vortices originated from the micro-ramps as well as the interaction of shock waves with the large-scale structures, are revealed and compared. Based on the time-correlation images, the temporal and spatial evolutionary characteristics of the coherent structures are investigated. It is beneficial to understand the dynamic mechanisms of the separated flow and the control mechanisms of the micro-ramps. The size of the separation region is determined by the NPLS and PIV. The results indicate that the control of the micro-ramps is capable of delaying the separation and diminishing the extent of recirculation zone.

A multiscale SPH particle model of the near-wall dynamics of leukocytes in flow.

PubMed

Gholami, Babak; Comerford, Andrew; Ellero, Marco

2014-01-01

A novel multiscale Lagrangian particle solver based on SPH is developed with the intended application of leukocyte transport in large arteries. In such arteries, the transport of leukocytes and red blood cells can be divided into two distinct regions: the bulk flow and the near-wall region. In the bulk flow, the transport can be modeled on a continuum basis as the transport of passive scalar concentrations. Whereas in the near-wall region, specific particle tracking of the leukocytes is required and lubrication forces need to be separately taken into account. Because of large separation of spatio-temporal scales involved in the problem, simulations of red blood cells and leukocytes are handled separately. In order to take the exchange of leukocytes between the bulk fluid and the near-wall region into account, solutions are communicated through coupling of conserved quantities at the interface between these regions. Because the particle tracking is limited to those leukocytes lying in the near-wall region only, our approach brings considerable speedup to the simulation of leukocyte circulation in a test geometry of a backward-facing step, which encompasses many flow features observed in vivo. Copyright © 2013 John Wiley & Sons, Ltd.

Characterization of Unsteady Flow Structures Around Tandem Cylinders for Component Interaction Studies in Airframe Noise

NASA Technical Reports Server (NTRS)

Jenkins, Luther N.; Khorrami, Mehdi R.; Choudhari, Meelan M.; McGinley, Catherine B.

2005-01-01

A joint computational and experimental study has been performed at NASA Langley Research Center to investigate the unsteady flow generated by the components of an aircraft landing gear system. Because the flow field surrounding a full landing gear is so complex, the study was conducted on a simplified geometry consisting of two cylinders in tandem arrangement to isolate and characterize the pertinent flow phenomena. This paper focuses on the experimental effort where surface pressures, 2-D Particle Image Velocimetry, and hot-wire anemometry were used to document the flow interaction around the two cylinders at a Reynolds Number of 1.66 x 10(exp 5), based on cylinder diameter, and cylinder spacing-todiameter ratios, L/D, of 1.435 and 3.70. Transition strips were applied to the forward cylinder to produce a turbulent boundary layer upstream of the flow separation. For these flow conditions and L/D ratios, surface pressures on both the forward and rear cylinders show the effects of L/D on flow symmetry, base pressure, and the location of flow separation and attachment. Mean velocities and instantaneous vorticity obtained from the PIV data are used to examine the flow structure between and aft of the cylinders. Shedding frequencies and spectra obtained using hot-wire anemometry are presented. These results are compared with unsteady, Reynolds-Averaged Navier-Stokes (URANS) computations for the same configuration in a companion paper by Khorrami, Choudhari, Jenkins, and McGinley (2005). The experimental dataset produced in this study provides information to better understand the mechanisms associated with component interaction noise, develop and validate time-accurate computer methods used to calculate the unsteady flow field, and assist in modeling of the radiated noise from landing gears.

Closed Loop Active Flow Separation Detection and Control in a Multistage Compressor

NASA Technical Reports Server (NTRS)

Bright, Michelle M.; Culley, Dennis E.; Braunscheidel, Edward P.; Welch, Gerard E.

2005-01-01

Active closed loop flow control was successfully demonstrated on a full annulus of stator vanes in a low speed axial compressor. Two independent methods of detecting separated flow conditions on the vane suction surface were developed. The first technique detects changes in static pressure along the vane suction surface, while the second method monitors variation in the potential field of the downstream rotor. Both methods may feasibly be used in future engines employing embedded flow control technology. In response to the detection of separated conditions, injection along the suction surface of each vane was used. Injected mass flow on the suction surface of stator vanes is known to reduce separation and the resulting limitation on static pressure rise due to lowered diffusion in the vane passage. A control algorithm was developed which provided a proportional response of the injected mass flow to the degree of separation, thereby minimizing the performance penalty on the compressor system.

Particle separation

NASA Technical Reports Server (NTRS)

Arnott, W. Patrick (Inventor); Chakrabarty, Rajan K. (Inventor); Moosmuller, Hans (Inventor)

2011-01-01

Embodiments of a method for selecting particles, such as based on their morphology, is disclosed. In a particular example, the particles are charged and acquire different amounts of charge, or have different charge distributions, based on their morphology. The particles are then sorted based on their flow properties. In a specific example, the particles are sorted using a differential mobility analyzer, which sorts particles, at least in part, based on their electrical mobility. Given a population of particles with similar electrical mobilities, the disclosed process can be used to sort particles based on the net charge carried by the particle, and thus, given the relationship between charge and morphology, separate the particles based on their morphology.

Particle separation

DOEpatents

Moosmuller, Hans [Reno, NV; Chakrabarty, Rajan K [Reno, NV; Arnott, W Patrick [Reno, NV

2011-04-26

Embodiments of a method for selecting particles, such as based on their morphology, is disclosed. In a particular example, the particles are charged and acquire different amounts of charge, or have different charge distributions, based on their morphology. The particles are then sorted based on their flow properties. In a specific example, the particles are sorted using a differential mobility analyzer, which sorts particles, at least in part, based on their electrical mobility. Given a population of particles with similar electrical mobilities, the disclosed process can be used to sort particles based on the net charge carried by the particle, and thus, given the relationship between charge and morphology, separate the particles based on their morphology.

Quantitative Magnetic Separation of Particles and Cells using Gradient Magnetic Ratcheting

PubMed Central

Murray, Coleman; Pao, Edward; Tseng, Peter; Aftab, Shayan; Kulkarni, Rajan; Rettig, Matthew; Di Carlo, Dino

2016-01-01

Extraction of rare target cells from biosamples is enabling for life science research. Traditional rare cell separation techniques, such as magnetic activated cell sorting (MACS), are robust but perform coarse, qualitative separations based on surface antigen expression. We report a quantitative magnetic separation technology using high-force magnetic ratcheting over arrays of magnetically soft micro-pillars with gradient spacing, and use the system to separate and concentrate magnetic beads based on iron oxide content (IOC) and cells based on surface expression. The system consists of a microchip of permalloy micro-pillar arrays with increasing lateral pitch and a mechatronic device to generate a cycling magnetic-field. Particles with higher IOC separate and equilibrate along the miro-pillar array at larger pitches. We develop a semi-analytical model that predicts behavior for particles and cells. Using the system, LNCaP cells were separated based on the bound quantity of 1μm anti-EpCAM particles as a metric for expression. The ratcheting cytometry system was able to resolve a ±13 bound particle differential, successfully distinguishing LNCaP from PC3 populations based on EpCAM expression, correlating with flow cytometry analysis. As a proof of concept, EpCAM-labeled cells from patient blood were isolated with 74% purity, demonstrating potential towards a quantitative magnetic separation instrument. PMID:26890496

Base flow (1966-2005) and streamflow gain and loss (2006) of the Brazos River, McLennan County to Fort Bend County, Texas

USGS Publications Warehouse

Turco, Michael J.; East, Jeffery W.; Milburn, Matthew S.

2007-01-01

During 2006?07, the U.S. Geological Survey (USGS), in cooperation with the Texas Water Development Board, did a study to quantify historical (water years 1966?2005) base flow and streamflow gains and losses from two streamflow-measuring surveys (March and August 2006) in the Brazos River from McLennan County to Fort Bend County, Texas. The Brazos River is hydraulically connected to the Brazos River alluvium aquifer, which in turn is hydraulically connected to several underlying aquifers, the outcrops of which occur in laterally adjacent layers generally parallel to the coast (major aquifers, Carrizo-Wilcox and Gulf Coast, and minor aquifers, Queen City, Sparta, and Yegua-Jackson). Hydrograph separation was done using the USGS computer program Hydrograph Separation and Analysis with historical streamflow from 10 USGS gaging stations, three on the Brazos River and seven on selected tributaries to the Brazos River. Streamflow data for computation of gains and losses were collected in March 2006 from 36 sites on the Brazos River and 19 sites on 19 tributaries to the Brazos River; and in August 2006 from 28 sites on the Brazos River and 16 sites on tributaries. Hydrograph separation and associated analyses indicate an appreciable increase in base flow as a percentage of streamflow in the reach of the Brazos River that crosses the outcrops of the Carrizo-Wilcox, Queen City, Sparta, and Yegua-Jackson aquifers compared to that in the adjacent upstream reach (on average from about 43 percent to about 60 percent). No increase in base flow as a percentage of streamflow in the reach of the Brazos River crossing the Gulf Coast aquifer compared to that in the adjacent upstream reach was indicated. Streamflow gains and losses computed for March 2006 for 35 reaches defined by pairs of sites on the Brazos River indicated that five reaches were verifiably gaining streamflow (computed gain exceeded potential flow measurement error) and none were verifiably losing streamflow. Four of the five gaining reaches are in the outcrop areas of the Carrizo-Wilcox and Yegua-Jackson aquifers. The results of the synoptic gain and loss surveys are consistent with the results of the base-flow analysis of historical streamflow. Appreciable increases in streamflow, apparently the result of increases in base flow, occur in the reach of the Brazos River that crosses the outcrops of the Carrizo-Wilcox, Queen City, Sparta, and Yegua-Jackson aquifers.

Validation of SIV measurements of turbulent characteristics in the separation region

NASA Astrophysics Data System (ADS)

Dushin, N. S.; Mikheev, N. I.; Dushina, O. A.; Zaripov, D. I.; Aslaev, A. K.

2017-11-01

Temporally and spatially resolved 2D measurements are important for the studies of complex turbulent flows. The recently developed SIV technique (Smoke Image Velocimetry), which is superior to PIV in some cases, can be used for this purpose. SIV validation results are presented for the steady turbulent backward-facing step flow measurements. Velocity profiles and Reynolds stress profiles are given for the regions of oncoming flow, reverse flow, flow reattachment and relaxation. The Reynolds number based on the step height and oncoming flow velocity at the boundary layer edge was Reh = 4834. The obtained data have been compared to LDA measurements and DNS.

Unsteady features of the flow on a bump in transonic environment

NASA Astrophysics Data System (ADS)

Budovsky, A. D.; Sidorenko, A. A.; Polivanov, P. A.; Vishnyakov, O. I.; Maslov, A. A.

2016-10-01

The study deals with experimental investigation of unsteady features of separated flow on a profiled bump in transonic environment. The experiments were conducted in T-325 wind tunnel of ITAM for the following flow conditions: P0 = 1 bar, T0 = 291 K. The base flow around the model was studied by schlieren visualization, steady and unsteady wall pressure measurements and PIV. The experimentally data obtained using PIV are analyzed by Proper Orthogonal Decomposition (POD) technique to investigate the underlying unsteady flow organization, as revealed by the POD eigenmodes. The data obtained show that flow pulsations revealed upstream and downstream of shock wave are correlated and interconnected.

Downstream Effects on Orbiter Leeside Flow Separation for Hypersonic Flows

NASA Technical Reports Server (NTRS)

Buck, Gregory M.; Pulsonetti, Maria V.; Weilmuenster, K. James

2005-01-01

Discrepancies between experiment and computation for shuttle leeside flow separation, which came to light in the Columbia accident investigation, are resolved. Tests were run in the Langley Research Center 20-Inch Hypersonic CF4 Tunnel with a baseline orbiter model and two extended trailing edge models. The extended trailing edges altered the wing leeside separation lines, moving the lines toward the fuselage, proving that wing trailing edge modeling does affect the orbiter leeside flow. Computations were then made with a wake grid. These calculations more closely matched baseline experiments. Thus, the present findings demonstrate that it is imperative to include the wake flow domain in CFD calculations in order to accurately predict leeside flow separation for hypersonic vehicles at high angles of attack.

Environmental continuous air monitor inlet with combined preseparator and virtual impactor

DOEpatents

Rodgers, John C [Santa Fe, NM

2007-06-19

An inlet for an environmental air monitor is described wherein a pre-separator interfaces with ambient environment air and removes debris and insects commonly associated with high wind outdoors and a deflector plate in communication with incoming air from the pre-separator stage, that directs the air radially and downward uniformly into a plurality of accelerator jets located in a manifold of a virtual impactor, the manifold being cylindrical and having a top, a base, and a wall, with the plurality of accelerator jets being located in the top of the manifold and receiving the directed air and accelerating directed air, thereby creating jets of air penetrating into the manifold, where a major flow is deflected to the walls of the manifold and extracted through ports in the walls. A plurality of receiver nozzles are located in the base of the manifold coaxial with the accelerator jets, and a plurality of matching flow restrictor elements are located in the plurality of receiver nozzles for balancing and equalizing the total minor flow among all the plurality of receiver nozzles, through which a lower, fractional flow extracts large particle constituents of the air for collection on a sample filter after passing through the plurality of receiver nozzles and the plurality of matching flow restrictor elements.

Flow processes in overexpanded chemical rocket nozzles. Part 3: Methods for the aimed flow separation and side load reduction

NASA Technical Reports Server (NTRS)

Schmucker, R. H.

1983-01-01

Methods aimed at reduction of overexpansion and side load resulting from asymmetric flow separation for rocket nozzles with a high opening ratio are described. The methods employ additional measures for nozzles with a fixed opening ratio. The flow separation can be controlled by several types of nozzle inserts, the properties of which are discussed. Side loads and overexpansion can be reduced by adapting the shape of the nozzle and taking other additional measures for controlled separation of the boundary layer, such as trip wires.

Use of DES in mildly separated internal flow: dimples in a turbulent channel

NASA Astrophysics Data System (ADS)

Tay, Chien Ming Jonathan; Khoo, Boo Cheong; Chew, Yong Tian

2017-12-01

Detached eddy simulation (DES) is investigated as a means to study an array of shallow dimples with depth to diameter ratios of 1.5% and 5% in a turbulent channel. The DES captures large-scale flow features relatively well, but is unable to predict skin friction accurately due to flow modelling near the wall. The current work instead relies on the accuracy of DES to predict large-scale flow features, as well as its well-documented reliability in predicting flow separation regions to support the proposed mechanism that dimples reduce drag by introducing spanwise flow components near the wall through the addition of streamwise vorticity. Profiles of the turbulent energy budget show the stabilising effect of the dimples on the flow. The presence of flow separation however modulates the net drag reduction. Increasing the Reynolds number can reduce the size of the separated region and experiments show that this increases the overall drag reduction.

An investigation into the mechanisms of drag reduction of a boat tailed base cavity on a blunt based body

NASA Astrophysics Data System (ADS)

Kehs, Joshua Paul

It is well documented in the literature that boat-tailed base cavities reduce the drag on blunt based bodies. The majority of the previous work has been focused on the final result, namely reporting the resulting drag reduction or base pressure increase without examining the methods in which such a device changes the fluid flow to enact such end results. The current work investigates the underlying physical means in which these devices change the flow around the body so as to reduce the overall drag. A canonical model with square cross section was developed for the purpose of studying the flow field around a blunt based body. The boat-tailed base cavity tested consisted of 4 panels of length equal to half the width of the body extending from the edges of the base at an angle towards the models center axis of 12°. Drag and surface pressure measurements were made at Reynolds numbers based on width from 2.3x105 to 3.6x10 5 in the Clarkson University high-speed wind tunnel over a range of pitch and yaw angles. Cross-stream hotwire wake surveys were used to identify wake width and turbulence intensities aft of the body at Reynolds numbers of 2.3x105 to 3.0x105. Particle Image Velocimetry (PIV) was used to quantify the flow field in the wake of the body, including the mean flow, vorticity, and turbulence measurements. The results indicated that the boat-tailed aft cavity decreases the drag significantly due to increased pressure on the base. Hotwire measurements indicated a reduction in wake width as well as a reduction in turbulence in the wake. PIV measurements indicated a significant reduction in wake turbulence and revealed that there exists a co-flowing stream that exits the cavity parallel to the free stream, reducing the shear in the flow at the flow separation point. The reduction in shear at the separation point indicated the method by which the turbulence was reduced. The reduction in turbulence combined with the reduction in wake size provided the mechanism of drag reduction by limiting the rate of entrainment of fluid in the recirculating wake to the free stream and by limiting the area over which this entrainment occurs.

Electrophoresis device

NASA Technical Reports Server (NTRS)

Rhodes, P. H.; Snyder, R. S. (Inventor)

1982-01-01

A device for separating cellular particles of a sample substance into fractionated streams of different cellular species includes a casing having a distribution chamber, a separation chamber, and a collection chamber. The electrode chambers are separated from the separation chamber interior by means of passages such that flow variations and membrane variations around the slotted portion of the electrode chamber do not enduce flow perturbations into the laminar buffer curtain flowing in the separation chamber. The cellular particles of the sample are separated under the influence of the electrical field and the separation chamber into streams of different cellular species. The streams of separated cells enter a partition array in the collection chamber where they are fractionated and collected.

Measurements in a separation bubble on an airfoil using laser velocimetry

NASA Technical Reports Server (NTRS)

Fitzgerald, Edward J.; Mueller, Thomas J.

1990-01-01

An experimental investigation was conducted to measure the reverse flow within the transitional separation bubble that forms on an airfoil at low Reynolds numbers. Measurements were used to determine the effect of the reverse flow on integrated boundary-layer parameters often used to model the bubble. Velocity profile data were obtained on an NACA 663-018 airfoil at angle of attack of 12 deg and a chord Reynolds number of 140,000 using laser Doppler and single-sensor hot-wire anemometry. A new correlation is proposed based on zero velocity position, since the Schmidt (1986) correlations fail in the turbulent portion of the bubble.

Physical mechanisms in shock-induced turbulent separated flow

NASA Astrophysics Data System (ADS)

Dolling, D. S.

1987-12-01

It has been demonstrated that the flow downstream of the moving shock is separated and that the foot of the shock is effectively the instantaneous separation point. The shock induced turbulent separation is an intermittant process and the separation line indicated by surface tracer methods, such as kerosene-lampblack, is a downstream boundary of a region of intermittent separation.

Simultaneous Multiple-Location Separation Control

NASA Technical Reports Server (NTRS)

Greenblatt, David (Inventor)

2009-01-01

A method of controlling a shear layer for a fluid dynamic body introduces first periodic disturbances into the fluid medium at a first flow separation location. Simultaneously, second periodic disturbances are introduced into the fluid medium at a second flow separation location. A phase difference between the first and second periodic disturbances is adjusted to control flow separation of the shear layer as the fluid medium moves over the fluid dynamic body.

Theoretical evaluation of engine auxiliary inlet design for supersonic V/STOL aircraft

NASA Technical Reports Server (NTRS)

Boles, Michael A.; Heavner, Richard L.

1988-01-01

A higher order panel method is used to evaluate the potential flow of a two dimensional supersonic V/STOL inlet. A non-symmetric analytical inlet model is developed to closely match a wind tunnel model. The analytical inlet is analyzed for flow characteristics around the lower cowl lip and auxiliary inlets. The results are obtained from the output of a computer program that is based on the Hess Panel Method which determines source strengths of panels distributed over a three dimensional body. The analytical model was designed for the implementation of drooped/translated cowl lip and auxiliary inlets as flow improvement concepts. A 40 or 70 degree droop lip can be incorporated on the inlet to determine if these geometry modifications result in flow improvements which may reduce the propensity for flow separation on the interior portion of the lip. Auxiliary inlets are employed to decrease the mass flow over the inlet lip. Thus, the peak flow velocity is reduced at the lip which also lessens the likelihood of flow separation on the interior portion of the lip. A 2, 4, and 6 inch translated lip can be employed to also decrease mass flow over the inlet lower lip in the same manner as the auxiliary inlet. The performance results of the flow improvement concepts show that three possible inlet configurations provide a situation where separation is less likely to occur. A 70 degree droop lip maintains flow conditions such that attached flow over the lower cowl lip may exist for the entire angle of attack range studied. A 0 degree droop and translated lip combination provides similar results for the angle of attack range. The third configuration consists of a 0 degree droop and auxiliary inlet combination. This configuration provides slightly less favorable results than the other two, but still allows for conditions favorable to attached flow within the inlet.

Flow and heat transfer in an L-shaped cooling passage with ribs and pin fins for the trailing edge of a gas-turbine vane and blade

NASA Astrophysics Data System (ADS)

Pardeshi, Irsha

Efficient and effective cooling of the trailing edges of gas-turbine vanes and blades is challenging because there is very little space to work with. In this study, CFD simulations based on steady RANS closed by the shear-stress transport turbulence model were performed to study the flow and heat transfer in an L-shaped duct for the trailing edge under two operating conditions. One operating condition, referred to as the laboratory condition, where experimental measurements were made, has a Reynolds number at the duct inlet of ReD = 15,000, coolant inlet temperature of Tinlet = 300 K, wall temperature of Twall = 335 K, a back pressure of Pb = 1 atm. When rotating, the angular speed was O = 1,000 rpm. The other condition, referred to as the engine-relevant condition, has Re D = 150,000 at the duct inlet, Tinlet = 673 K, Twall = 1,173 K, and Pb = 25 atm. When rotating, O was 3,600 rpm. The objective is to understand the nature of the flow and heat transfer in an L-shaped cooling passage for the trailing edge that has a combination of ribs and pin fins under rotating and non-rotating conditions with focus on how pin fins and ribs distribute the flow throughout the passage and to understand what features of the flow and heat transfer can or cannot be extrapolated from the laboratory to the engine-relevant operating conditions. When there is no rotation, results obtained show that for both operating conditions, the pin fins minimized the size of the separation bubble when the flow exits the inlet duct into the expanded portion of the L-shaped duct. The size of the separation bubble at the tip of the L-shaped duct created by the adverse pressure gradient is quite large for the laboratory condition and relatively small for the engine condition. Each rib was found to create two sets of recirculating flows, one just upstream of the rib because of the adverse pressure gradient induced by the rib and one just downstream of the rib because of flow separation from a sharp edge. These recirculating flows spiral from the ribs towards the exit of the L-shaped duct, and the spiraling brings cool fluid from the middle of the passage to the walls. Each pin fin was found to induce a pair of counter-rotating separated regions behind it and has horse-shoe vortices that wrap around it next to the top and bottom walls. The heat transfer is highest just upstream of the each rib, around the pin fins, and when the cooling fluid impinges on walls, and very low in the separated region next to the tip. When there is rotation, Coriolis force creates a pair of counter-rotating vortices that bring the cooler fluid to the trailing wall in the inlet duct. Thus, the trailing wall has higher heat transfer than the leading wall. In the inlet duct, centrifugal buoyancy causes a massive flow separation on the leading wall. In the expanded portion of the L-shaped duct, the centrifugal-buoyancy-induced separation on the leading wall is limited to the region with the ribs, and the separation degenerates into a series of smaller spiraling separation bubbles, one between every set of consecutive ribs. On the leading and trailing walls, the ribs and the pin fins induce the same kind of flows as they did under non-rotating conditions. Because of centrifugal-buoyancy-induced flow separation on the leading face, the heat transfer on the leading wall is 10-15% lower than that on the trailing wall, which is not significant. The adverse effects of centrifugal buoyancy were mitigated because the separation bubbles between the ribs are spiraling from the side wall to the trailing-edge exit and are constantly supplied by new coolant. The heat transfer on the side and back walls is higher near the trailing wall because centrifugal buoyancy directed most of the coolant flow towards the trailing wall. The size of the separation bubble at the tip of the L-shaped duct essentially disappeared when there is rotation for both the lab and engine-relevant conditions.

A study of the compressible flow through a diffusing S-duct

NASA Technical Reports Server (NTRS)

Wellborn, Steven R.; Okiishi, Theodore H.; Reichert, Bruce A.

1993-01-01

Benchmark aerodynamic data are presented for compressible flow through a representative S-duct configuration. A numerical prediction of the S-duct flow field, obtained from a subsonic parabolized Navier-Stokes algorithm, is also shown. The experimental and numerical results are compared. Measurements of the three-dimensional velocity field, total pressures, and static pressures were obtained at five cross-sectional planes. Aerodynamic data were gathered with calibrated pneumatic probes. Surface static pressure and surface flow visualization data were also acquired. All reported tests were conducted with an inlet centerline Mach number of 0.6. The Reynolds number, based on the inlet centerline velocity and duct inlet diameter, was 2.6 x 10(exp 6). Thin inlet turbulent boundary layers existed. The collected data should be beneficial to aircraft inlet designers and the measurements are suitable for the validation of computational codes. The results show that a region of streamwise flow separation occurred within the duct. Details about the separated flow region, including mechanisms which drive this complicated flow phenomenon, are discussed. Results also indicate that the duct curvature induces strong pressure driven secondary flows. The cross flows evolve into counter-rotating vortices. These vortices convect low momentum fluid of the boundary layer toward the center of the duct, degrading both the uniformity and magnitude of the total pressure profile.

Analysis of Aerospike Plume Induced Base-Heating Environment

NASA Technical Reports Server (NTRS)

Wang, Ten-See

1998-01-01

Computational analysis is conducted to study the effect of an aerospike engine plume on X-33 base-heating environment during ascent flight. To properly account for the effect of forebody and aftbody flowfield such as shocks and to allow for potential plume-induced flow-separation, thermo-flowfield of trajectory points is computed. The computational methodology is based on a three-dimensional finite-difference, viscous flow, chemically reacting, pressure-base computational fluid dynamics formulation, and a three-dimensional, finite-volume, spectral-line based weighted-sum-of-gray-gases radiation absorption model computational heat transfer formulation. The predicted convective and radiative base-heat fluxes are presented.

Application of an Upwind High Resolution Finite-Differencing Scheme and Multigrid Method in Steady-State Incompressible Flow Simulations

NASA Technical Reports Server (NTRS)

Yang, Cheng I.; Guo, Yan-Hu; Liu, C.- H.

1996-01-01

The analysis and design of a submarine propulsor requires the ability to predict the characteristics of both laminar and turbulent flows to a higher degree of accuracy. This report presents results of certain benchmark computations based on an upwind, high-resolution, finite-differencing Navier-Stokes solver. The purpose of the computations is to evaluate the ability, the accuracy and the performance of the solver in the simulation of detailed features of viscous flows. Features of interest include flow separation and reattachment, surface pressure and skin friction distributions. Those features are particularly relevant to the propulsor analysis. Test cases with a wide range of Reynolds numbers are selected; therefore, the effects of the convective and the diffusive terms of the solver can be evaluated separately. Test cases include flows over bluff bodies, such as circular cylinders and spheres, at various low Reynolds numbers, flows over a flat plate with and without turbulence effects, and turbulent flows over axisymmetric bodies with and without propulsor effects. Finally, to enhance the iterative solution procedure, a full approximation scheme V-cycle multigrid method is implemented. Preliminary results indicate that the method significantly reduces the computational effort.

Comparison of Turbulence Models for Nozzle-Afterbody Flows with Propulsive Jets

NASA Technical Reports Server (NTRS)

Compton, William B., III

1996-01-01

A numerical investigation was conducted to assess the accuracy of two turbulence models when computing non-axisymmetric nozzle-afterbody flows with propulsive jets. Navier-Stokes solutions were obtained for a Convergent-divergent non-axisymmetric nozzle-afterbody and its associated jet exhaust plume at free-stream Mach numbers of 0.600 and 0.938 at an angle of attack of 0 deg. The Reynolds number based on model length was approximately 20 x 10(exp 6). Turbulent dissipation was modeled by the algebraic Baldwin-Lomax turbulence model with the Degani-Schiff modification and by the standard Jones-Launder kappa-epsilon turbulence model. At flow conditions without strong shocks and with little or no separation, both turbulence models predicted the pressures on the surfaces of the nozzle very well. When strong shocks and massive separation existed, both turbulence models were unable to predict the flow accurately. Mixing of the jet exhaust plume and the external flow was underpredicted. The differences in drag coefficients for the two turbulence models illustrate that substantial development is still required for computing very complex flows before nozzle performance can be predicted accurately for all external flow conditions.

Experimental investigation of the draft tube inlet flow of a bulb turbine

NASA Astrophysics Data System (ADS)

Vuillemard, J.; Aeschlimann, V.; Fraser, R.; Lemay, S.; Deschênes, C.

2014-03-01

In the BulbT project framework, a bulb turbine model was studied with a strongly diverging draft tube. At high discharge, flow separation occurs in the draft tube correlated to significant efficiency and power drops. In this context, a focus was put on the draft tube inlet flow conditions. Actually, a precise inlet flow velocity field is required for comparison and validation purposes with CFD simulation. This paper presents different laser Doppler velocimetry (LDV) measurements at the draft tube inlet and their analysis. The LDV was setup to measure the axial and circumferential velocity on a radius under the runner and a diameter under the hub. A method was developed to perform indirect measurement of the mean radial velocity component. Five operating conditions were studied to correlate the inlet flow to the separation in the draft tube. Mean velocities, fluctuations and frequencies allowed characterizing the flow. Using this experimental database, the flow structure was characterized. Phase averaged velocities based on the runner position allowed detecting the runner blade wakes. The velocity gradients induced by the blade tip vortices were captured. The guide vane wakes was also detected at the draft tube inlet. The recirculation in the hub wake was observed.

Biased Cyclical Electrical Field-Flow Fractionation for Separation of Submicron Particles

PubMed Central

Ornthai, Mathuros; Siripinyanond, Atitaya; Gale, Bruce K.

2015-01-01

The potential of biased cyclical electrical field flow fractionation (BCyElFFF), which applies the positive cycle voltage longer than the negative cycle voltage, for characterization of submicron particles, was investigated. Parameters affecting separation and retention such as voltage, frequency, and duty cycle were examined. The results suggest that the separation mechanism in BCyElFFF in many cases is more related to the size of particles, as is the case with normal ElFFF, in the studied conditions, than the electrophoretic mobility, which is what the theory predicts for CyElFFF. However, better resolution was obtained when separating using BCyElFFF mode than when using normal CyElFFF. BCyElFFF was able to demonstrate simultaneous baseline separations of a mixture of 0.04, 0.1, and 0.2 μm particles and near separation of 0.5 μm particles. This study has shown the applicability of the BCyElFFF for separation and characterization of submicron particles greater than 0.1 μm in size, which had not been demonstrated previously. The separation and retention results suggest that for particles of this size, retention is based more on particle size than on electrophoretic mobility, which is contrary to existing theory for CyElFFF. PMID:26612733

Biased cyclical electrical field-flow fractionation for separation of submicron particles.

PubMed

Ornthai, Mathuros; Siripinyanond, Atitaya; Gale, Bruce K

2016-01-01

The potential of biased cyclical electrical field-flow fractionation (BCyElFFF), which applies the positive cycle voltage longer than the negative cycle voltage, for characterization of submicron particles, was investigated. Parameters affecting separation and retention such as voltage, frequency, and duty cycle were examined. The results suggest that the separation mechanism in BCyElFFF in many cases is more related to the size of particles, as is the case with normal ElFFF, in the studied conditions, than the electrophoretic mobility, which is what the theory predicts for CyElFFF. However, better resolution was obtained when separating using BCyElFFF mode than when using normal CyElFFF. BCyElFFF was able to demonstrate simultaneous baseline separations of a mixture of 0.04-, 0.1-, and 0.2-μm particles and near separation of 0.5-μm particles. This study has shown the applicability of BCyElFFF for separation and characterization of submicron particles greater than 0.1-μm in size, which had not been demonstrated previously. The separation and retention results suggest that for particles of this size, retention is based more on particle size than on electrophoretic mobility, which is contrary to existing theory for CyElFFF.

Fluid Dynamic Mechanisms and Interactions within Separated Flows and Their Effects on Missile Aerodynamics

DTIC Science & Technology

1983-05-01

Mechanics, Vol. 43, S~ries E, No. 3, September 1976, pp. 387-395. 18. Warpinski , N. R. and Chow, W. L., "Base Pressure Associated With Incompressible...Flow Past Wedges at High Reynolds Numbers," J. of Applied Mechanics, Vol. 46, No. 3, September 1979. 19. Warpinski , N. R. and Chow, W. L., "Viscid

Experimental Study of Transitional Flow Behavior in a Simulated Low Pressure Turbine

NASA Technical Reports Server (NTRS)

Sohn, Ki Hyeon; DeWitt, Kenneth J.

1998-01-01

A detailed investigation of the flow physics occurring on the suction side of a simulated Low Pressure Turbine (LPT) blade was performed. A contoured upper wall was designed to simulate the pressure distribution of an actual LPT airfoil onto a flat lower plate. The experiments were carried out for the Reynolds numbers of 35,000, 70,000, 100,000 and 250,000 with four levels of freestream turbulence ranging from 1% to 4%. For the three lower Reynolds numbers, the boundary layer on the flat plate was separated and formed a bubble. The size of laminar separation bubble was measured to be inversely proportional to the freestream turbulence levels and Reynolds numbers. However, no separation was observed for the Re = 250,000 case. The transition on a separated flow was found to proceed through the formation of turbulent spots in the free shear layer as evidenced in the intermittency profiles for Re = 35,000, 70,000 and 100,000. Spectral data show no evidence of Kelvin-Helmholtz or Tollmien-Schlichting instability waves in the free shear layer over a separation bubble (bypass transition). However, the flow visualization revealed the large vortex structures just outside of the bubble and their development to turbulent flow for Re = 50,000, which is similar to that in the free shear layer (separated-flow transition). Therefore, it is fair to say that the bypass and separated-flow transition modes coexist in the transitional flows over the separation bubble for certain conditions. Transition onset and end locations and length determined from intermittency profiles decrease as Reynolds number and freestream turbulence levels increase.

Experimental Study of Transitional Flow Behavior in a Simulated Low Pressure Turbine

NASA Technical Reports Server (NTRS)

Sohn, Ki Hyeon; DeWitt, Kenneth J.

2007-01-01

A detailed investigation of the flow physics occurring on the suction side of a simulated Low Pressure Turbine (LPT) blade was performed. A contoured upper wall was designed to simulate the pressure distribution of an actual LPT airfoil onto a flat lower plate. The experiments were carried out for the Reynolds numbers of 35,000, 70,000, 100,000, and 250,000 with four levels of freestream turbulence ranging from 1 to 4 percent. For the three lower Reynolds numbers, the boundary layer on the flat plate was separated and formed a bubble. The size of laminar separation bubble was measured to be inversely proportional to the freestream turbulence levels and Reynolds numbers. However, no separation was observed for the Re = 250,000 case. The transition on a separated flow was found to proceed through the formation of turbulent spots in the free shear layer as evidenced in the intermittency profiles for Re = 35,000, 70,000, and 100,000. Spectral data show no evidence of Kelvin-Helmholtz of Tollmien-Schlichting instability waves in the free shear layer over a separation bubble (bypass transition). However, the flow visualization revealed the large vortex structures just outside of the bubble and their development to turbulent flow for Re = 50,000, which is similar to that in the free shear layer (separated-flow transition). Therefore, it is fair to say that the bypass and separated-flow transition modes coexist in the transition flows over the separation bubble of certain conditions. Transition onset and end locations and length determined from intermittency profiles decreased as Reynolds number and freestream turbulence levels increase.

On-chip free-flow magnetophoresis: continuous flow separation of magnetic particles and agglomerates.

PubMed

Pamme, Nicole; Manz, Andreas

2004-12-15

The separation of magnetic microparticles was achieved by on-chip free-flow magnetophoresis. In continuous flow, magnetic particles were deflected from the direction of laminar flow by a perpendicular magnetic field depending on their magnetic susceptibility and size and on the flow rate. Magnetic particles could thus be separated from each other and from nonmagnetic materials. Magnetic and nonmagnetic particles were introduced into a microfluidic separation chamber, and their deflection was studied under the microscope. The magnetic particles were 2.0 and 4.5 microm in diameter with magnetic susceptibilities of 1.12 x 10(-4) and 1.6 x 10(-4) m(3) kg(-1), respectively. The 4.5-microm particles with the larger susceptibility were deflected further from the direction of laminar flow than the 2.0-microm magnetic particles. Nonmagnetic 6-microm polystyrene beads, however, were not deflected at all. Furthermore, agglomerates of magnetic particles were found to be deflected to a larger extent than single magnetic particles. The applied flow rate and the strength and gradient of the applied magnetic field were the key parameters in controlling the deflection. This separation method has a wide applicability since magnetic particles are commonly used in bioanalysis as a solid support material for antigens, antibodies, DNA, and even cells. Free-flow magnetophoretic separations could be hyphenated with other microfluidic devices for reaction and analysis steps to form a micro total analysis system.

Bistable flow occurrence in the 2D model of a steam turbine valve

NASA Astrophysics Data System (ADS)

Pavel, Procházka; Václav, Uruba

2017-09-01

The internal flow inside a steam turbine valve was investigated experimentally using PIV measurement. The valve model was proposed to be two-dimensional. The model was connected to the blow-down wind tunnel. The flow conditions were set by the different position of the valve plug. Several angles of the diffuser by diverse radii were investigated concerning flow separation and flow dynamics. It was found that the flow takes one of two possible bistable modes. The first regime is characterized by a massive flow separation just at the beginning of the diffuser section on the one side. The second regime is axisymmetric and the flow separation is not detected at all.

Fluid dynamic problems associated with air-breathing propulsive systems

NASA Technical Reports Server (NTRS)

Chow, W. L.

1979-01-01

A brief account of research activities on problems related to air-breathing propulsion is made in this final report for the step funded research grant NASA NGL 14-005-140. Problems include the aircraft ejector-nozzle propulsive system, nonconstant pressure jet mixing process, recompression and reattachment of turbulent free shear layer, supersonic turbulent base pressure, low speed separated flows, transonic boattail flow with and without small angle of attack, transonic base pressures, Mach reflection of shocks, and numerical solution of potential equation through hodograph transformation.

The NASA Low-Pressure Turbine Flow Physics Program

NASA Technical Reports Server (NTRS)

Ashpis, David E.

1998-01-01

An overview of the NASA Lewis Low-Pressure Turbine (LPT) Flow Physics Program will be presented. The program was established in response to the aero-engine industry's need for improved LPT efficiency and designs. Modern jet engines have four to seven LPT stages, significantly contributing to engine weight. In addition, there is a significant efficiency degradation between takeoff and cruise conditions, of up to 2 points. Reducing the weight and part count of the LPT and minimizing the efficiency degradation will translate into fuel savings. Accurate prediction methods of LPT flows and losses are needed to accomplish those improvements. The flow in LPT passages is at low Reynolds number, and is dominated by interplay of three basic mechanisms: transition, separation and wake interaction. The affecting parameters traditionally considered are Reynolds number, freestream turbulence intensity, wake frequency parameter, and the pressure distribution (loading). Three-dimensional effects and additional parameters, particularly turbulence characteristics like length scales, spectra and other statistics, as well as wake turbulence intensity and properties also play a role. The flow of most interest is on the suction surface, where large losses are generated as the flow tends to separate at the low Reynolds numbers. Ignoring wakes, a common flow scenario, there is laminar separation, followed by transition on the separation bubble and turbulent reattachment. If transition starts earlier the separation will be eliminated and the boundary layer will be attached leading to the well known bypass transition issues. In contrast, transition over a separation bubble is closer to free shear layer transition and was not investigated as well, particularly in the turbine environment. Unsteadiness created by wakes complicates the picture. Wakes induce earlier transition, and the calmed regions trailing the induced turbulent spots can delay or eliminate separation via shear stress modification. Three-dimensional flow physics and geometry will have strong effects. Altogether a very complex and challenging problem emerges. The objective of the program is to provide improved models and physical understanding of the complex flow, which are essential for accurate prediction of flow and losses in the LPT. Experimental, computational and analytical work as complementing and augmenting approaches are used. The program involves industry, universities and research institutes, and other government laboratories. It is characterized by strong interaction among participants, quick dissemination of results, and responsiveness to industry's needs. The presentation will describe the work elements. Highlighting some activities in progress are experiments on simulated blade suction surface in low-speed wind tunnels, on curved wall, and on a flat-plate, both with pressure gradient. In the area of computation, assessment of existing models is performed using RANS (Reynolds Averaged Navier Stokes) simulations. Laminar flow DNS was completed. Analytical studies of instability and receptivity in attached and separated flows were started. In the near future the program is moving to include wake effects and development of improved modeling. Experimental work in preparation stages are: (1) Addition of wakes to the curved tunnel experiment; (2) Low-speed rotating rig experiment on GE90 engine LPT; and (3) Transonic cascade. In the area of computation, it is expected to move from model assessment towards development of improved models. In addition, a new project of Large Eddy Simulation (LES) of LPT is to begin and will provide numerical data bases. It is planned to implement the emerging improved models in a multistage turbomachinery code and to validate against the GE90 engine LPT.

Quantifying components of the hydrologic cycle in Virginia using chemical hydrograph separation and multiple regression analysis

USGS Publications Warehouse

Sanford, Ward E.; Nelms, David L.; Pope, Jason P.; Selnick, David L.

2012-01-01

This study by the U.S. Geological Survey, prepared in cooperation with the Virginia Department of Environmental Quality, quantifies the components of the hydrologic cycle across the Commonwealth of Virginia. Long-term, mean fluxes were calculated for precipitation, surface runoff, infiltration, total evapotranspiration (ET), riparian ET, recharge, base flow (or groundwater discharge) and net total outflow. Fluxes of these components were first estimated on a number of real-time-gaged watersheds across Virginia. Specific conductance was used to distinguish and separate surface runoff from base flow. Specific-conductance data were collected every 15 minutes at 75 real-time gages for approximately 18 months between March 2007 and August 2008. Precipitation was estimated for 1971–2000 using PRISM climate data. Precipitation and temperature from the PRISM data were used to develop a regression-based relation to estimate total ET. The proportion of watershed precipitation that becomes surface runoff was related to physiographic province and rock type in a runoff regression equation. Component flux estimates from the watersheds were transferred to flux estimates for counties and independent cities using the ET and runoff regression equations. Only 48 of the 75 watersheds yielded sufficient data, and data from these 48 were used in the final runoff regression equation. The base-flow proportion for the 48 watersheds averaged 72 percent using specific conductance, a value that was substantially higher than the 61 percent average calculated using a graphical-separation technique (the USGS program PART). Final results for the study are presented as component flux estimates for all counties and independent cities in Virginia.

Broadening of Analyte Streams due to a Transverse Pressure Gradient in Free-Flow Isoelectric Focusing

PubMed Central

Dutta, Debashis

2017-01-01

Pressure-driven cross-flows can arise in free-flow isoelectric focusing systems (FFIEF) due to a non-uniform electroosmotic flow velocity along the channel width induced by the pH gradient in this direction. In addition, variations in the channel cross-section as well as unwanted differences in hydrostatic heads at the buffer/sample inlet ports can also lead to such pressure-gradients which besides altering the equilibrium position of the sample zones have a tendency to substantially broaden their widths deteriorating the separations. In this situation, a thorough assessment of stream broadening due to transverse pressure-gradients in FFIEF devices is necessary in order to establish accurate design rules for the assay. The present article describes a mathematical framework to estimate the noted zone dispersion in FFIEF separations based on the method-of-moments approach under laminar flow conditions. A closed-form expression has been derived for the spatial variance of the analyte streams at their equilibrium positions as a function of the various operating parameters governing the assay performance. This expression predicts the normalized stream variance under the chosen conditions to be determined by two dimensionless Péclet numbers evaluated based on the transverse pressure-driven and electrophoretic solute velocities in the separation chamber, respectively. Moreover, the analysis shows that while the stream width can be expected to increase with an increase in the value of the first Péclet number, the opposite trend will be followed with respect to the latter. The noted results have been validated using Monte Carlo simulations that also establish a time/length scale over which the predicted equilibrium stream width is attained in the system. PMID:28081900

Circulation Plasma Centrifuge with Product Flow

NASA Astrophysics Data System (ADS)

Borisevich, V. D.; Potanin, E. P.

2018-05-01

We have analyzed the isotope separation in a high-frequency plasma circulating centrifuge operating with a product flow. The rotation of a weakly ionized plasma is ensured by a rotating magnetic field, while the countercurrent flow (circulation) is produced by a traveling magnetic field. We have calculated the dependences of the enrichment factor and the separative power of the centrifuge on a product flow. The optimal characteristics of the separation unit have been determined.

Turbulence Simulation of Laboratory Wind-Wave Interaction in High Winds and Upscaling to Ocean Conditions

DTIC Science & Technology

2016-12-22

investigated air-sea fluxes characterized by strong air flow separation over a very steep wave field. We first investigated propagating steep wave...mechanisms for flow separation over rigid surfaces compared with unsteady surfaces with a boundary slip velocity. We investigated passive scalar fluxes. In...turbulent flow over steep stationary roughness, the primary mechanism for momentum flux is via pressure drag resulting from flow separation. However

Thin-layer approximation and algebraic model for separated turbulent flows

NASA Technical Reports Server (NTRS)

Baldwin, B.; Lomax, H.

1978-01-01

An algebraic turbulence model for two- and three-dimensional separated flows is specified that avoids the necessity for finding the edge of the boundary layer. Properties of the model are determined and comparisons made with experiment for an incident shock on a flat plate, separated flow over a compression corner, and transonic flow over an airfoil. Separation and reattachment points from numerical Navier-Stokes solutions agree with experiment within one boundary-layer thickness. Use of law-of-the-wall boundary conditions does not alter the predictions significantly. Applications of the model to other cases are contained in companion papers.

Corona anemometry for qualitative measurement of reversing surface flow with application to separation control by external excitation

NASA Technical Reports Server (NTRS)

Durbin, P. A.; Mckinzie, D. J.

1987-01-01

An corona anemometer which detects gas flow by the displacement of an ion beam is described, and experiments are performed using the anemometer to investigate the active control of diffusor separation by periodic forcing. The apparatus is applied to the separated flow over a rearward facing ramp. An oscillating vane is attached to the surface near the separation point. It is suggested that the enhancement in turbulent energy produced by the oscillating vane is due to drastic modification of the wake shear flow, and not to vane-produced turbulence.

Particle image velocimetry based on wavelength division multiplexing

NASA Astrophysics Data System (ADS)

Tang, Chunxiao; Li, Enbang; Li, Hongqiang

2018-01-01

This paper introduces a technical approach of wavelength division multiplexing (WDM) based particle image velocimetry (PIV). It is designed to measure transient flows with different scales of velocity by capturing multiple particle images in one exposure. These images are separated by different wavelengths, and thus the pulse separation time is not influenced by the frame rate of the camera. A triple-pulsed PIV system has been created in order to prove the feasibility of WDM-PIV. This is demonstrated in a sieve plate extraction column model by simultaneously measuring the fast flow in the downcomer and the slow vortices inside the plates. A simple displacement/velocity field combination method has also been developed. The constraints imposed by WDM-PIV are limited wavelength choices of available light sources and cameras. The usage of WDM technique represents a feasible way to realize multiple-pulsed PIV.

A cell-vertex multigrid method for the Navier-Stokes equations

NASA Technical Reports Server (NTRS)

Radespiel, R.

1989-01-01

A cell-vertex scheme for the Navier-Stokes equations, which is based on central difference approximations and Runge-Kutta time stepping, is described. Using local time stepping, implicit residual smoothing, a multigrid method, and carefully controlled artificial dissipative terms, very good convergence rates are obtained for a wide range of two- and three-dimensional flows over airfoils and wings. The accuracy of the code is examined by grid refinement studies and comparison with experimental data. For an accurate prediction of turbulent flows with strong separations, a modified version of the nonequilibrium turbulence model of Johnson and King is introduced, which is well suited for an implementation into three-dimensional Navier-Stokes codes. It is shown that the solutions for three-dimensional flows with strong separations can be dramatically improved, when a nonequilibrium model of turbulence is used.

Flow separation of currents in shallow water

USGS Publications Warehouse

Signell, Richard P.

1989-01-01

Flow separation of currents in shallow coastal areas is investigated using a boundary layer model for two-dimensional (depth-averaged) tidal flow past an elliptic headland. If the shoaling region near the coast is narrow compared to the scale of the headland, bottom friction causes the flow to separate just downstream of the point where the pressure gradient switches from favoring to adverse. As long as the shoaling region at the coast is well resolved, the inclusion of eddy viscosity and a no-slip boundary condition have no effect on this result. An approximate analytic solution for the pressure gradient along the boundary is obtained by assuming the flow away from the immediate vicinity of the boundary is irrotational. On the basis of the pressure gradient obtained from the irrotational flow solution, flow separation is a strong function of the headland aspect ratio, an equivalent Reynolds number, and a Keulegan-Carpenter number.

Application of Sweeping Jet Actuators on the NASA Hump Model and Comparison with CFDVAL2004 Experiments

NASA Technical Reports Server (NTRS)

Koklu, Mehti

2017-01-01

Flow separation control over a wall-mounted hump model was studied experimentally to assess the performance of sweeping jet actuators. Results were compared to that of the 2004 CFD validation experiment (CFDVAL2004), which examined flow separation control with steady suction and unsteady zero-net-mass-flow actuators. Comparisons were carried out at low and high amplitude excitations. In addition to the active flow control methods, a passive flow control method (i.e., vortex generator) was used to complement the dataset. Steady/unsteady surface pressure measurements and surface oilflow visualization were used in the performance assessment of the actuators. The results indicated that the sweeping jet actuators are more effective than the steady suction and unsteady zero-net-mass-flow actuators. For the same momentum coefficient, the sweeping jet actuators produced more flow acceleration upstream of separation, more pressure recovery downstream, and consistently a smaller separation bubble.

Influence of a three-phase separator configuration on the performance of an upflow anaerobic sludge bed reactor treating wastewater from a fruit-canning factory.

PubMed

Wongnoi, Rachbordin; Songkasiri, Warinthorn; Phalakornkule, Chantaraporn

2007-02-01

The objective of this study was to investigate the influence of a three-phase separator configuration on the performance of an upflow anaerobic sludge bed (USAB) treating wastewater from a fruit canning factory. The performances of two 30-L UASB reactors--one with a modified three-phase separator giving a spiral flow pattern and the other with a conventional configuration-were investigated in parallel. Wastewater, with a chemical oxygen demand (COD) concentration between 2000 and 7000 mg/L, was obtained from a fruit-canning factory. Based on the effluent data of the first 100 operation days, the UASB with the three-phase separator giving spiral flow patterns yielded up to 25% lower biomass washout. It also showed better efficiencies in treating wastewater--up to 60% lower effluent COD, up to 20% higher COD percent removal, and up to 29% higher biogas production. This work presents evidence of an improvement on the conventional physical design of a UASB.

Tessellated permanent magnet circuits for flow-through, open gradient separations of weakly magnetic materials

PubMed Central

Moore, Lee R.; Williams, P. Stephen; Chalmers, Jeffrey J.; Zborowski, Maciej

2017-01-01

Emerging microfluidic-based cell assays favor label-free red blood cell (RBC) depletion. Magnetic separation of RBC is possible because of the paramagnetism of deoxygenated hemoglobin but the process is slow for open-gradient field configurations. In order to increase the throughput, periodic arrangements of the unit magnets were considered, consisting of commercially available Nd-Fe-B permanent magnets and soft steel flux return pieces. The magnet design is uniquely suitable for multiplexing by magnet tessellation, here meaning the tiling of the magnet assembly cross-sectional plane by periodic repetition of the magnet and the flow channel shapes. The periodic pattern of magnet magnetizations allows a reduction of the magnetic material per channel with minimal distortion of the field cylindrical symmetry inside the magnet apertures. A number of such magnet patterns are investigated for separator performance, size and economy with the goal of designing an open-gradient magnetic separator capable of reducing the RBC number concentration a hundred-fold in 1 mL whole blood per hour. PMID:29104346

Tessellated permanent magnet circuits for flow-through, open gradient separations of weakly magnetic materials.

PubMed

Moore, Lee R; Williams, P Stephen; Chalmers, Jeffrey J; Zborowski, Maciej

2017-04-01

Emerging microfluidic-based cell assays favor label-free red blood cell (RBC) depletion. Magnetic separation of RBC is possible because of the paramagnetism of deoxygenated hemoglobin but the process is slow for open-gradient field configurations. In order to increase the throughput, periodic arrangements of the unit magnets were considered, consisting of commercially available Nd-Fe-B permanent magnets and soft steel flux return pieces. The magnet design is uniquely suitable for multiplexing by magnet tessellation, here meaning the tiling of the magnet assembly cross-sectional plane by periodic repetition of the magnet and the flow channel shapes. The periodic pattern of magnet magnetizations allows a reduction of the magnetic material per channel with minimal distortion of the field cylindrical symmetry inside the magnet apertures. A number of such magnet patterns are investigated for separator performance, size and economy with the goal of designing an open-gradient magnetic separator capable of reducing the RBC number concentration a hundred-fold in 1 mL whole blood per hour.

Low-Frequency Flow Oscillations on Stalled Wings Exhibiting Cellular Separation Topology

NASA Astrophysics Data System (ADS)

Disotell, Kevin James

One of the most pervasive threats to aircraft controllability is wing stall, a condition associated with loss of lift due to separation of air flow from the wing surface at high angles of attack. A recognized need for improved upset recovery training in extended-envelope flight simulators is a physical understanding of the post-stall aerodynamic environment, particularly key flow phenomena which influence the vehicle trajectory. Large-scale flow structures known as stall cells, which scale with the wing chord and are spatially-periodic along the span, have been previously observed on post-stall airfoils with trailing-edge separation present. Despite extensive documentation of stall cells in the literature, the physical mechanisms behind their formation and evolution have proven to be elusive. The undertaken study has sought to characterize the inherently turbulent separated flow existing above the wing surface with cell formation present. In particular, the question of how the unsteady separated flow may interact with the wing to produce time-averaged cellular surface patterns is considered. Time-resolved, two-component particle image velocimetry measurements were acquired at the plane of symmetry of a single stall cell formed on an extruded NACA 0015 airfoil model at chord Reynolds number of 560,000 to obtain insight into the time-dependent flow structure. The evolution of flow unsteadiness was analyzed over a static angle-of-attack range covering the narrow post-stall regime in which stall cells have been observed. Spectral analysis of velocity fields acquired near the stall angle confirmed a low-frequency flow oscillation previously detected in pointwise surface measurements by Yon and Katz (1998), corresponding to a Strouhal number of 0.042 based on frontal projected chord height. Probability density functions of the streamwise velocity component were used to estimate the convective speed of this mode at approximately half the free-stream velocity, in agreement with Yon and Katz. Large-amplitude streamwise Reynolds stresses in the separated shear layer were found to be manifested by the low-frequency oscillation through inspection of the spectral energy distribution. Using the method of Proper Orthogonal Decomposition to construct reduced-order models of the acquired time sequences, the low-frequency unsteadiness appeared to be linked to an interaction between the separated and trailing-edge shear layers, in contrast to a bubble-bursting mechanism which has been observed for different stall behaviors. As the static angle of attack was increased further, the separated flow structure was seen to transition to a faster eddy motion expected for bluff-body wakes. A novel scaling study was conducted to evaluate the potential role of low-frequency unsteadiness in producing the spanwise wavelengths associated with cell formation, which was found to be in favorable agreement with scaling trends in the literature. Finally, instantaneous pressure-sensitive paint measurements were demonstrated on a DU 97-W-300 wind turbine airfoil at chord Reynolds number of 225,000 with leading-edge trip applied, in which the development of spiral node structures associated with cell formation were captured in the trailing-edge separation. The contributed work suggests that further study into the influence of large-scale unsteadiness on the three-dimensional organization of stall cells is merited.

Stormflow-hydrograph separation based on isotopes: the thrill is gone--what's next?

USGS Publications Warehouse

Burns, Douglas A.

2002-01-01

Beginning in the 1970s, the promise of a new method for separatingstormflow hydrographs using18O,2H, and3Hprovedanirresistibletemptation, and was a vast improvement over graphical separationand solute tracer methods that were prevalent at the time. Eventu-ally, hydrologists realized that this new method entailed a plethoraof assumptions about temporal and spatial homogeneity of isotopiccomposition (many of which were commonly violated). Nevertheless,hydrologists forged ahead with dozens of isotope-based hydrograph-separation studies that were published in the 1970s and 1980s.Hortonian overland flow was presumed dead. By the late 1980s,the new isotope-based hydrograph separation technique had movedinto adolescence, accompanied by typical adolescent problems suchas confusion and a search for identity. As experienced hydrologistscontinued to use the isotope technique to study stormflow hydrol-ogy in forested catchments in humid climates, their younger peersfollowed obligingly—again and again. Was Hortonian overland flowreally dead and forgotten, though? What about catchments in whichpeople live and work? And what about catchments in dry climatesand the tropics? How useful were study results when several of theassumptions about the homogeneity of source waters were commonlyviolated? What if two components could not explain the variation ofisotopic composition measured in the stream during stormflow? Andwhat about uncertainty? As with many new tools, once the initialshine wore off, the limitations of the method became a concern—oneof which was that isotope-based hydrograph separations alone couldnot reveal much about the flow paths by which water arrives at astream channel during storms.

Control of low-speed turbulent separated flow over a backward-facing ramp. Ph.D. Thesis - Old Dominion Univ.

NASA Technical Reports Server (NTRS)

Lin, John C.

1992-01-01

The relative performance and flow phenomena associated with several devices for controlling turbulent separated flow were investigated at low speeds. Relative performance of the devices was examined for flow over a curved, backward-facing ramp in a wind tunnel, and the flow phenomena were examined in a water tunnel using dye-flow visualization. Surface static pressure measurements and oil-flow visualization results from the wind tunnel tests indicated that transverse grooves, longitudinal grooves, submerged vortex generators, vortex generator jets (VGJ's), Viets' fluidic flappers, elongated arches at positive angle of attack, and large-eddy breakup devices (LEBU's) at positive angle of attack placed near the baseline separation location reduce flow separation and increase pressure recovery. Spanwise cylinders reduce flow separation but decrease pressure recovery downstream. Riblets, passive porous surfaces, swept grooves, Helmholtz resonators, and arches and LEBU's with angle of attack less than or = 0 degrees had no significant effect in reducing the extent of the separation region. Wall-cooling computations indicated that separation delay on a partially-cooled ramp is nearly the same as on a fully-cooled ramp, while minimizing the frictional drag increase associated with the wall cooling process. Dry-flow visualization tests in the water tunnel indicated that wishbone vortex generators in the forward orientation shed horseshoe vortices; wishbone vortex generators oriented in the reverse direction and doublet vortex generators shed streamwise counterrotating vortices; a spanewise cylinder located near the wall and LEBU's at angle of attack = -10 degrees produced eddies or transverse vortices which rotated with the same sign as the mean vorticity in a turbulent boundary layer; and the most effective VGJ's produced streamwise co-rotating vortices. Comparative wind-tunnel test results indicated that transferring momentum from the outer region of a turbulent boundary layer through the action of embedded streamwise vortices is more effective than by transverse vortices for the separation control application studied herein.

Experimental Investigation of Transition to Turbulence as Affected By Passing Wakes

NASA Technical Reports Server (NTRS)

Kaszeta, Richard W.; Ashpis, David E.; Simon, Terrence W.

2001-01-01

This paper presents experimental results from a study of the effects of periodically passing wakes upon laminar-to-turbulent transition and separation in a low-pressure turbine passage. The test section geometry is designed to simulate unsteady wakes in turbine engines for studying their effects on boundary layers and separated flow regions over the suction surface by using a single suction surface and a single pressure surface to simulate a single turbine blade passage. Single-wire, thermal anemometry techniques are used to measure time-resolved and phase averaged, wall-normal profiles of velocity, turbulence intensity and intermittency at multiple streamwise locations over the turbine airfoil suction surface. These data are compared to steady-state wake-free data collected in the same geometry to identify the effects of wakes upon laminar-to-turbulent transition. Results are presented for flows with a Reynolds number based on suction surface length and stage exit velocity of 50,000 and an approach flow turbulence intensity of 2.5%. While both existing design and experimental data are primarily concerned with higher Reynolds number flows (Re greater than 100,000), recent advances in gas turbine engines, and the accompanying increase in laminar and transitional flow effects, have made low-Re research increasingly important. From the presented data, the effects of passing wakes on transition and separation in the boundary layer, due to both increased turbulence levels and varying streamwise pressure gradients are presented. The results show how the wakes affect transition. The wakes affect the flow by virtue of their difference in turbulence levels and scales from those of the free-stream and by virtue of their ensemble- averaged velocity deficits, relative to the free-stream velocity, and the concomitant changes in angle of attack and temporal pressure gradients. The relationships between the velocity oscillations in the freestream and the unsteady velocity profile shapes in the near-wall flow are described. In this discussion is support for the theory that bypass transition is a response of the near-wall viscous layer to pressure fluctuations imposed upon it from the free-stream flow. Recent transition models are based on that premise. The data also show a significant lag between when the wake is present over the surface and when transition begins.cous layer to pressure fluctuations imposed upon it from the free-stream flow. Recent transition models are based on that premise. The data also show a significant lag between when the wake is present over the surface and when transition begins.cous layer to pressure fluctuations imposed upon it from the free-stream flow. Recent transition models are based on that premise. The data also show a significant lag between when the wake is present over the surface and when transition begins.

Simulations of Bluff Body Flow Interaction for Noise Source Modeling

NASA Technical Reports Server (NTRS)

Khorrami, Medi R.; Lockard David P.; Choudhari, Meelan M.; Jenkins, Luther N.; Neuhart, Dan H.; McGinley, Catherine B.

2006-01-01

The current study is a continuation of our effort to characterize the details of flow interaction between two cylinders in a tandem configuration. This configuration is viewed to possess many of the pertinent flow features of the highly interactive unsteady flow field associated with the main landing gear of large civil transports. The present effort extends our previous two-dimensional, unsteady, Reynolds Averaged Navier-Stokes computations to three dimensions using a quasilaminar, zonal approach, in conjunction with a two-equation turbulence model. Two distinct separation length-to-diameter ratios of L/D = 3.7 and 1.435, representing intermediate and short separation distances between the two cylinders, are simulated. The Mach 0.166 simulations are performed at a Reynolds number of Re = 1.66 105 to match the companion experiments at NASA Langley Research Center. Extensive comparisons with the measured steady and unsteady surface pressure and off-surface particle image velocimetry data show encouraging agreement. Both prominent and some of the more subtle trends in the mean and fluctuating flow fields are correctly predicted. Both computations and the measured data reveal a more robust and energetic shedding process at L/D = 3.7 in comparison with the weaker shedding in the shorter separation case of L/D = 1.435. The vortex shedding frequency based on the computed surface pressure spectra is in reasonable agreement with the measured Strouhal frequency.

A real-time control strategy for separation of highly polluted storm water based on UV-Vis online measurements--from theory to operation.

PubMed

Hoppe, H; Messmann, S; Giga, A; Gruening, H

2011-01-01

'Classical' real-time control (RTC) strategies in sewer systems are based on water level and flow measurements with the goal of activation of retention volume. The control system rule of 'clean (storm water) runoff into the receiving water - polluted runoff into the treatment plant' has been thwarted by rough operating conditions and lack of measurements. Due to the specific boundary conditions in the city of Wuppertal's separate sewer system (clean stream water is mixed with polluted storm water runoff) a more sophisticated--pollution-based--approach was needed. In addition the requirements to be met by the treatment of storm water runoff have become more stringent in recent years. To separate the highly-polluted storm water runoff during rain events from the cleaner stream flow a pollution-based real-time control (P-RTC) system was developed and installed. This paper describes the measurement and P-RTC equipment, the definition of total suspended solids as the pollution-indicating parameter, the serviceability of the system, and also gives a cost assessment. A sensitivity analysis and pollution load calculations have been carried out in order to improve the P-RTC algorithm. An examination of actual measurements clearly shows the ecological and economic advantages of the P-RTC strategy.

In-flight flow visualization results from the X-29A aircraft at high angles of attack

NASA Technical Reports Server (NTRS)

Delfrate, John H.; Saltzman, John A.

1992-01-01

Flow visualization techniques were used on the X-29A aircraft at high angles of attack to study the vortical flow off the forebody and the surface flow on the wing and tail. The forebody vortex system was studied because asymmetries in the vortex system were suspected of inducing uncommanded yawing moments at zero sideslip. Smoke enabled visualization of the vortex system and correlation of its orientation with flight yawing moment data. Good agreement was found between vortex system asymmetries and the occurrence of yawing moments. Surface flow on the forward-swept wing of the X-29A was studied using tufts and flow cones. As angle of attack increased, separated flow initiated at the root and spread outboard encompassing the full wing by 30 deg angle of attack. In general, the progression of the separated flow correlated well with subscale model lift data. Surface flow on the vertical tail was also studied using tufts and flow cones. As angle of attack increased, separated flow initiated at the root and spread upward. The area of separated flow on the vertical tail at angles of attack greater than 20 deg correlated well with the marked decrease in aircraft directional stability.

Flow control in a diffusing S-Duct

NASA Technical Reports Server (NTRS)

Vakili, A. D.; Wu, J. M.; Liver, P.; Bhat, M. K.

1985-01-01

Accurate measurements have been made of secondary flow in a 1.51 area ratio diffusing 30 deg - 30 deg S-Duct with circulair cross section. Turbulent flow was entering the duct at Mach number of 0.6, the boundary layer thickness at the duct entrance was ten percent of the duct inlet diameter. Through measurements made, local flow velocity vector as well as static and total pressures mapping of the flow at several stations were obtained. Strong secondary flow was measured in the first bend which continued into the second bend with new vorticity produced in there in the opposite direction. Surface oil flow visualization and wall pressures indicated a region of separated flow starting at theta approximately equal to 22 deg on the inside of the first bend up to theta approximately equal to 44 deg on the outside of the second bend. The flow separated in 'cyclone' form and never reattached in the duct. As a result of the secondary flow and the flow separation, significant total pressure distortion was observed at the exit of the duct. Using flow control devices the separation was eliminated while the exit distortion was improved.

The effect of initial flow nonuniformity on second-stage fuel injection and combustion in a supersonic duct. [supersonic combustion ramjet engine

NASA Technical Reports Server (NTRS)

Russin, W. R.

1975-01-01

The effects of flow nonuniformity on second-stage hydrogen fuel injection and combustion in supersonic flow were evaluated. The first case, second-stage fuel injection into a uniform duct flow, produced data indicating that fuel mixing is considerably slower than estimates based on an empirical mixing correlation. The second-case, two-stage fuel injection (or second-stage fuel injection into a nonuniform duct flow), produced a large interaction between stages with extensive flow separation. For this case the measured wall pressure, heat transfer, and amount of reaction at the duct exit were significantly greater than estimates based on the mixing correlation. Substantially more second-stage fuel burned in the second case than in the first case. Overall effects of unmixedness/chemical kinetics were found not to be significant at the exit for stoichiometric fuel injection.

Atomic Force Microscope Mediated Chromatography

NASA Technical Reports Server (NTRS)

Anderson, Mark S.

2013-01-01

The atomic force microscope (AFM) is used to inject a sample, provide shear-driven liquid flow over a functionalized substrate, and detect separated components. This is demonstrated using lipophilic dyes and normal phase chromatography. A significant reduction in both size and separation time scales is achieved with a 25-micron-length column scale, and one-second separation times. The approach has general applications to trace chemical and microfluidic analysis. The AFM is now a common tool for ultra-microscopy and nanotechnology. It has also been demonstrated to provide a number of microfluidic functions necessary for miniaturized chromatography. These include injection of sub-femtoliter samples, fluidic switching, and sheardriven pumping. The AFM probe tip can be used to selectively remove surface layers for subsequent microchemical analysis using infrared and tip-enhanced Raman spectroscopy. With its ability to image individual atoms, the AFM is a remarkably sensitive detector that can be used to detect separated components. These diverse functional components of microfluidic manipulation have been combined in this work to demonstrate AFM mediated chromatography. AFM mediated chromatography uses channel-less, shear-driven pumping. This is demonstrated with a thin, aluminum oxide substrate and a non-polar solvent system to separate a mixture of lipophilic dyes. In conventional chromatographic terms, this is analogous to thin-layer chromatography using normal phase alumina substrate with sheardriven pumping provided by the AFM tip-cantilever mechanism. The AFM detection of separated components is accomplished by exploiting the variation in the localized friction of the separated components. The AFM tip-cantilever provides the mechanism for producing shear-induced flows and rapid pumping. Shear-driven chromatography (SDC) is a relatively new concept that overcomes the speed and miniaturization limitations of conventional liquid chromatography. SDC is based on a sliding plate system, consisting of two flat surfaces, one of which has a recessed channel. A fluid flow is produced by axially sliding one plate past another, where the fluid has mechanical shear forces imposed at each point along the channel length. The shear-induced flow rates are very reproducible, and do not have pressure or voltage gradient limitations. SDC opens up a new range of enhanced separation kinetics by permitting the sample confinement with submicron dimensions. Small, highly confined liquid is advantageous for chromatographic separation because the separation rate is known to scale according to the square of the confined sample diameter. In addition, because shear-driven flows are not limited by fluid velocity, shear-driven liquid chromatography may provide up to 100,000 plate efficiency.

The effect of large aspect ratio wing yaw on active separation control

NASA Astrophysics Data System (ADS)

Tewes, Philipp; Taubert, Lutz; Wygnanski, Israel

2014-11-01

The applicability of the boundary layer independence principle to turbulent boundary layers developing on infinitely yawed wings, suggested that active separation control might be carried out differently to the two presumably independent developing boundary layers. At low incidence or flap deflection the control of the spanwise component of the flow is effective provided the aggregate number of actuators is small. In this case the actuator jets provide jet-curtains that virtually eliminate the spanwise flow component of the flow in their vicinity. At higher incidence or flap deflection, the focus of the active separation control has to shift to the chordwise component that has to overcome a high adverse pressure gradient. The idea was proven experimentally on a flapped wing based on a NACA 0012 airfoil that could be swept back and forward while being suspended from a ceiling of a wind tunnel connected to a six-component balance. The experiments were carried out at Reynolds numbers varying between 300,000 and 500,000. The project was supported in part by a grant from AFOSR.

Study of unsteady flow simulation of backward impeller with non-uniform casing

NASA Astrophysics Data System (ADS)

Swe, War War Min; Morimatsu, Hiroya; Hayashi, Hidechito; Okumura, Tetsuya; Oda, Ippei

2017-06-01

The flow characteristics of the centrifugal fans with different blade outlet angles are basically discussed on steady and unsteady simulations for a rectangular casing fan. The blade outlet angles of the impellers are 35° and 25° respectively. The unsteady flow behavior in the passage of the impeller 35° is quite different from that in the steady flow behavior. The large flow separation occurs in the steady flow field and unsteady flow field of the impeller 35°, the flow distribution in the circumferential direction varies remarkably and the flow separation on the blade occurs only at the back region of the fan; but the steady flow behavior in the impeller 25° is almost consistent with the unsteady flow behavior, the flow distribution of the circumferential direction doesn't vary much and the flow separation on the blade hardly occurs. When the circumferential variation of the flow in the impeller is large, the steady flow simulation is not coincident to the unsteady flow simulation.

The numerical calculation of laminar boundary-layer separation

NASA Technical Reports Server (NTRS)

Klineberg, J. M.; Steger, J. L.

1974-01-01

Iterative finite-difference techniques are developed for integrating the boundary-layer equations, without approximation, through a region of reversed flow. The numerical procedures are used to calculate incompressible laminar separated flows and to investigate the conditions for regular behavior at the point of separation. Regular flows are shown to be characterized by an integrable saddle-type singularity that makes it difficult to obtain numerical solutions which pass continuously into the separated region. The singularity is removed and continuous solutions ensured by specifying the wall shear distribution and computing the pressure gradient as part of the solution. Calculated results are presented for several separated flows and the accuracy of the method is verified. A computer program listing and complete solution case are included.

Enhancement of microfluidic particle separation using cross-flow filters with hydrodynamic focusing

PubMed Central

Chiu, Yun-Yen; Huang, Chen-Kang

2016-01-01

A microfluidic chip is proposed to separate microparticles using cross-flow filtration enhanced with hydrodynamic focusing. By exploiting a buffer flow from the side, the microparticles in the sample flow are pushed on one side of the microchannels, lining up to pass through the filters. Meanwhile a larger pressure gradient in the filters is obtained to enhance separation efficiency. Compared with the traditional cross-flow filtration, our proposed mechanism has the buffer flow to create a moving virtual boundary for the sample flow to actively push all the particles to reach the filters for separation. It further allows higher flow rates. The device only requires soft lithograph fabrication to create microchannels and a novel pressurized bonding technique to make high-aspect-ratio filtration structures. A mixture of polystyrene microparticles with 2.7 μm and 10.6 μm diameters are successfully separated. 96.2 ± 2.8% of the large particle are recovered with a purity of 97.9 ± 0.5%, while 97.5 ± 0.4% of the small particle are depleted with a purity of 99.2 ± 0.4% at a sample throughput of 10 μl/min. The experiment is also conducted to show the feasibility of this mechanism to separate biological cells with the sample solutions of spiked PC3 cells in whole blood. By virtue of its high separation efficiency, our device offers a label-free separation technique and potential integration with other components, thereby serving as a promising tool for continuous cell filtration and analysis applications. PMID:26858812

Analysis of cellular autofluorescence in touch samples by flow cytometry: implications for front end separation of trace mixture evidence.

PubMed

Katherine Philpott, M; Stanciu, Cristina E; Kwon, Ye Jin; Bustamante, Eduardo E; Greenspoon, Susan A; Ehrhardt, Christopher J

2017-07-01

The goal of this study was to survey optical and biochemical variation in cell populations deposited onto a surface through touch or contact and identify specific features that may be used to distinguish and then sort cell populations from separate contributors in a trace biological mixture. Although we were not able to detect meaningful biochemical variation in touch samples deposited by different contributors through preliminary antibody surveys, we did observe distinct differences in red autofluorescence emissions (650-670 nm), with as much as a tenfold difference in mean fluorescence intensities observed between certain pairs of donors. Results indicate that the level of red autofluorescence in touch samples can be influenced by a donor's contact with specific material prior to handling the substrate from which cells were collected. In particular, we observed increased red autofluorescence in cells deposited subsequent to handling laboratory gloves, plant material, and certain types of marker ink, which could be easily visualized microscopically or using flow cytometry, and persisted after hand washing. To test whether these observed optical differences could potentially be used as the basis for a cell separation workflow, a controlled two-person touch mixture was separated into two fractions via fluorescence-activated cell sorting (FACS) using gating criteria based on intensity of 650-670 nm emissions and then subjected to DNA analysis. Genetic analysis of the sorted fractions provided partial DNA profiles that were consistent with separation of individual contributors from the mixture suggesting that variation in autofluorescence signatures, even if driven by extrinsic factors, may nonetheless be a useful means of isolating contributors to some touch mixtures. Graphical Abstract Conceptual workflow diagram. Trace biological mixtures containing cells from multiple individuals are analyzed by flow cytometry. Cells are then physically separated into two populations based on intensity of red autofluorescence using Fluorescence Activated Cell Sorting. Each isolated cell fraction is subjected to DNA analysis resulting in a DNA profile for each contributor.

Predicting streamflow regime metrics for ungauged streamsin Colorado, Washington, and Oregon

NASA Astrophysics Data System (ADS)

Sanborn, Stephen C.; Bledsoe, Brian P.

2006-06-01

Streamflow prediction in ungauged basins provides essential information for water resources planning and management and ecohydrological studies yet remains a fundamental challenge to the hydrological sciences. A methodology is presented for stratifying streamflow regimes of gauged locations, classifying the regimes of ungauged streams, and developing models for predicting a suite of ecologically pertinent streamflow metrics for these streams. Eighty-four streamflow metrics characterizing various flow regime attributes were computed along with physical and climatic drainage basin characteristics for 150 streams with little or no streamflow modification in Colorado, Washington, and Oregon. The diverse hydroclimatology of the study area necessitates flow regime stratification and geographically independent clusters were identified and used to develop separate predictive models for each flow regime type. Multiple regression models for flow magnitude, timing, and rate of change metrics were quite accurate with many adjusted R2 values exceeding 0.80, while models describing streamflow variability did not perform as well. Separate stratification schemes for high, low, and average flows did not considerably improve models for metrics describing those particular aspects of the regime over a scheme based on the entire flow regime. Models for streams identified as 'snowmelt' type were improved if sites in Colorado and the Pacific Northwest were separated to better stratify the processes driving streamflow in these regions thus revealing limitations of geographically independent streamflow clusters. This study demonstrates that a broad suite of ecologically relevant streamflow characteristics can be accurately modeled across large heterogeneous regions using this framework. Applications of the resulting models include stratifying biomonitoring sites and quantifying linkages between specific aspects of flow regimes and aquatic community structure. In particular, the results bode well for modeling ecological processes related to high-flow magnitude, timing, and rate of change such as the recruitment of fish and riparian vegetation across large regions.

Loss reduction in axial-flow compressors through low-speed model testing

NASA Technical Reports Server (NTRS)

Wisler, D. C.

1984-01-01

A systematic procedure for reducing losses in axial-flow compressors is presented. In this procedure, a large, low-speed, aerodynamic model of a high-speed core compressor is designed and fabricated based on aerodynamic similarity principles. This model is then tested at low speed where high-loss regions associated with three-dimensional endwall boundary layers flow separation, leakage, and secondary flows can be located, detailed measurements made, and loss mechanisms determined with much greater accuracy and much lower cost and risk than is possible in small, high-speed compressors. Design modifications are made by using custom-tailored airfoils and vector diagrams, airfoil endbends, and modified wall geometries in the high-loss regions. The design improvements resulting in reduced loss or increased stall margin are then scaled to high speed. This paper describes the procedure and presents experimental results to show that in some cases endwall loss has been reduced by as much as 10 percent, flow separation has been reduced or eliminated, and stall margin has been substantially improved by using these techniques.

Performance of Reynolds Averaged Navier-Stokes Models in Predicting Separated Flows: Study of the Hump Flow Model Problem

NASA Technical Reports Server (NTRS)

Cappelli, Daniele; Mansour, Nagi N.

2012-01-01

Separation can be seen in most aerodynamic flows, but accurate prediction of separated flows is still a challenging problem for computational fluid dynamics (CFD) tools. The behavior of several Reynolds Averaged Navier-Stokes (RANS) models in predicting the separated ow over a wall-mounted hump is studied. The strengths and weaknesses of the most popular RANS models (Spalart-Allmaras, k-epsilon, k-omega, k-omega-SST) are evaluated using the open source software OpenFOAM. The hump ow modeled in this work has been documented in the 2004 CFD Validation Workshop on Synthetic Jets and Turbulent Separation Control. Only the baseline case is treated; the slot flow control cases are not considered in this paper. Particular attention is given to predicting the size of the recirculation bubble, the position of the reattachment point, and the velocity profiles downstream of the hump.

Numerical Analysis of Incipient Separation on 53 Deg Swept Diamond Wing

NASA Technical Reports Server (NTRS)

Frink, Neal T.

2015-01-01

A systematic analysis of incipient separation and subsequent vortex formation from moderately swept blunt leading edges is presented for a 53 deg swept diamond wing. This work contributes to a collective body of knowledge generated within the NATO/STO AVT-183 Task Group titled 'Reliable Prediction of Separated Flow Onset and Progression for Air and Sea Vehicles'. The objective is to extract insights from the experimentally measured and numerically computed flow fields that might enable turbulence experts to further improve their models for predicting swept blunt leading-edge flow separation. Details of vortex formation are inferred from numerical solutions after establishing a good correlation of the global flow field and surface pressure distributions between wind tunnel measurements and computed flow solutions. From this, significant and sometimes surprising insights into the nature of incipient separation and part-span vortex formation are derived from the wealth of information available in the computational solutions.

Propagations of fluctuations and flow separation on an unsteadily loaded airfoil

NASA Astrophysics Data System (ADS)

Tenney, Andrew; Lewalle, Jacques

2014-11-01

We analyze pressure data from 18 taps located along the surface of a DU-96-W180 airfoil in bothand steady flow conditions. The conditions were set to mimic the flow conditions experienced by a wind turbine blade under unsteady loading to test and to quantify the effects of several flow control schemes. Here we are interested in the propagation of fluctuations along the pressure and suction sides, particularly in relation to the fluctuating separation point. An unsteady phase of the incoming fluctuations is defined using Morlet wavelets, and phase-conditioned cross-correlations are calculated. Using wavelet-based pattern recognition, individual events in the pressure data are identified with several different algorithms utilizing both the original time series pressure signals and their corresponding scalograms. The data analyzed in this study was collected by G. Wang in the Skytop anechoic chamber at Syracuse University in the spring of 2013; the work of Zhe Bai on this data is also acknowledged.

Redox active polymers and colloidal particles for flow batteries

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gavvalapalli, Nagarjuna; Moore, Jeffrey S.; Rodriguez-Lopez, Joaquin

The invention provides a redox flow battery comprising a microporous or nanoporous size-exclusion membrane, wherein one cell of the battery contains a redox-active polymer dissolved in the non-aqueous solvent or a redox-active colloidal particle dispersed in the non-aqueous solvent. The redox flow battery provides enhanced ionic conductivity across the electrolyte separator and reduced redox-active species crossover, thereby improving the performance and enabling widespread utilization. Redox active poly(vinylbenzyl ethylviologen) (RAPs) and redox active colloidal particles (RACs) were prepared and were found to be highly effective redox species. Controlled potential bulk electrolysis indicates that 94-99% of the nominal charge on different RAPsmore » is accessible and the electrolysis products are stable upon cycling. The high concentration attainable (>2.0 M) for RAPs in common non-aqueous battery solvents, their electrochemical and chemical reversibility, and their hindered transport across porous separators make them attractive materials for non-aqueous redox flow batteries based on size-selectivity.« less

Turbulence measurements in hypersonic shock-wave boundary-layer interaction flows

NASA Technical Reports Server (NTRS)

Mikulla, V.; Horstman, C. C.

1976-01-01

Turbulent intensity and Reynolds shear stress measurements are presented for two nonadiabatic hypersonic shock-wave boundary-layer interaction flows, one with and one without separation. These measurements were obtained using a new hot-wire probe specially designed for heated flows. Comparison of the separated and attached flows shows a significant increase above equilibrium values in the turbulent intensity and shear stress downstream of the interaction region for the attached case, while for the separated case, the turbulent fluxes remain close to equilibrium values. This effect results in substantial differences in turbulence lifetime for the two flows. We propose that these differences are due to a coupling between the turbulent energy and separation bubble unsteadiness, a hypothesis supported by the statistical properties of the turbulent fluctuations.

Evaluation of the HF-Radar network system around Taiwan using normalized cumulative Lagrangian separation.

NASA Astrophysics Data System (ADS)

Fredj, Erick; Kohut, Josh; Roarty, Hugh; Lai, Jian-Wu

2017-04-01

The Lagrangian separation distance between the endpoints of simulated and observed drifter trajectories is often used to assess the performance of numerical particle trajectory models. However, the separation distance fails to indicate relative model performance in weak and strong current regions, such as over continental shelves and the adjacent deep ocean. A skill score described in detail by (Lui et.al. 2011) was applied to estimate the cumulative Lagrangian separation distances normalized by the associated cumulative trajectory lengths. In contrast, the Lagrangian separation distance alone gives a misleading result. The proposed dimensionless skill score is particularly useful when the number of drifter trajectories is limited and neither a conventional Eulerian-based velocity nor a Lagrangian based probability density function may be estimated. The skill score assesses The Taiwan Ocean Radar Observing System (TOROS) performance. TOROS consists of 17 SeaSonde type radars around the Taiwan Island. The currents off Taiwan are significantly influenced by the nearby Kuroshio current. The main stream of the Kuroshio flows along the east coast of Taiwan to the north throughout the year. Sometimes its branch current also bypasses the south end of Taiwan and goes north along the west coast of Taiwan. The Kuroshio is also prone to seasonal change in its speed of flow, current capacity, distribution width, and depth. The evaluations of HF-Radar National Taiwanese network performance using Lagrangian drifter records demonstrated the high quality and robustness of TOROS HF-Radar data using a purely trajectory-based non-dimensional index. Yonggang Liu and Robert H. Weisberg, "Evaluation of trajectory modeling in different dynamic regions using normalized cumulative Lagrangian separation", Journal of Geophysical Research, Vol. 116, C09013, doi:10.1029/2010JC006837, 2011

Fiber-based monolithic columns for liquid chromatography.

PubMed

Ladisch, Michael; Zhang, Leyu

2016-10-01

Fiber-based monoliths for use in liquid chromatographic separations are defined by columns packed with aligned fibers, woven matrices, or contiguous fiber structures capable of achieving rapid separations of proteins, macromolecules, and low molecular weight components. A common denominator and motivating driver for this approach, first initiated 25 years ago, was reducing the cost of bioseparations in a manner that also reduced residence time of retained components while achieving a high ratio of mass to momentum transfer. This type of medium, when packed into a liquid chromatography column, minimized the fraction of stagnant liquid and resulted in a constant plate height for non-adsorbing species. The uncoupling of dispersion from eluent flow rate enabled the surface chemistry of the stationary phase to be considered separately from fluid transport phenomena and pointed to new ways to apply chemistry for the engineering of rapid bioseparations. This paper addresses developments and current research on fiber-based monoliths and explains how the various forms of this type of chromatographic stationary phase have potential to provide new tools for analytical and preparative scale separations. The different stationary phases are discussed, and a model that captures the observed constant plate height as a function of mobile phase velocity is reviewed. Methods that enable hydrodynamically stable fiber columns to be packed and operated over a range of mobile phase flow rates, together with the development of new fiber chemistries, are shown to provide columns that extend the versatility of liquid chromatography using monoliths, particularly at the preparative scale. Graphical Abstract Schematic representation of a sample mixture being separated by a rolled-stationary phase column, resulting separated peaks shown in the chromatogram.

Silver and gold nanoparticle separation using asymmetrical flow-field flow fractionation: Influence of run conditions and of particle and membrane charges.

PubMed

Meisterjahn, Boris; Wagner, Stephan; von der Kammer, Frank; Hennecke, Dieter; Hofmann, Thilo

2016-04-01

Flow-Field Flow Fractionation (Flow-FFF), coupled with online detection systems is one of the most promising tools available for the separation and quantification of engineered nanoparticles (ENPs) in complex matrices. To correctly relate the retention of nanoparticles in the Flow-FFF-channel to the particle size, ideal separation conditions must be met. This requires optimization of the parameters that influence the separation behavior. The aim of this study was therefore to systematically investigate and evaluate the influence of parameters such as the carrier liquid, the cross flow, and the membrane material, on the separation behavior of two metallic ENPs. For this purpose the retention, recovery, and separation efficiency of sterically stabilized silver nanoparticles (AgNPs) and electrostatically stabilized gold nanoparticles (AuNPs), which represent two materials widely used in investigations on environmental fate and ecotoxicology, were investigated against a parameter matrix of three different cross-flow densities, four representative carrier solutions, and two membrane materials. The use of a complex mixture of buffers, ionic and non-ionic surfactants (FL-70 solution) together with a medium cross-flow density provided an acceptable compromise in peak quality and recovery for both types of ENPs. However, these separation conditions do not represent a perfect match for both particle types at the same time (maximized recovery at maximized retention). It could be shown that the behavior of particles within Flow-FFF channels cannot be predicted or explained purely in terms of electrostatic interactions. Particles were irreversibly lost under conditions where the measured zeta potentials suggested that there should have been sufficient electrostatic repulsion to ensure stabilization of the particles in the Flow-FFF channel resulting in good recoveries. The wide variations that we observed in ENP behavior under different conditions, together with the different behavior that has been reported in published literature for the same NPs under similar conditions, indicate a need for improvement in the membrane materials used for Flow-FFF analysis of NPs. This research has shown that careful adjustment of separation conditions can result in acceptable, but not ideal, separation conditions for two fundamentally different stabilized materials, and that it may not be possible to separate a set of different particles under ideal conditions for each particle type. This therefore needs to be taking into account in method development and when interpreting FFF results from complex samples. Copyright © 2016 Elsevier B.V. All rights reserved.

Turbine endwall single cylinder program

NASA Technical Reports Server (NTRS)

Langston, L. S.

1982-01-01

Detailed measurement of the flow field in front of a large-scale single cylinder, mounted in a wind tunnel is discussed. A better understanding of the three dimensional separation occuring in front of the cylinder on the endwall, and of the vortex system that is formed is sought. A data base with which to check analytical and numerical computer models of three dimensional flows is also anticipated.

Separate and combined sewer systems: a long-term modelling approach.

PubMed

Mannina, Giorgio; Viviani, Gaspare

2009-01-01

Sewer systems convey mostly dry weather flow, coming from domestic and industrial sanitary sewage as well as infiltration flow, and stormwater due to meteoric precipitations. Traditionally, in urban drainage two types of sewer systems are adopted: separate and combined sewers. The former convey dry and wet weather flow separately into two different networks, while the latter convey dry and wet weather flow together. Which is the best solution in terms of cost-benefit analysis still remains a controversial subject. The present study was aimed at comparing the pollution loads discharged to receiving bodies by Wastewater Treatment Plant (WWTP) and Combined Sewer Overflow (CSO) for different kinds of sewer systems (combined and separate). To accomplish this objective, a comparison between the two systems was carried out using results from simulations of catchments characterised by different dimensions, population densities and water supply rate. The analysis was based on a parsimonious mathematical model able to simulate the sewer system as well as the WWTP during both dry and wet weather. The rain series employed for the simulations was six years long. Several pollutants, both dissolved and particulate, were modelled. The results confirmed the uncertainties in the choice of one system versus the other, emphasising the concept that case-by-case solutions have to be undertaken. Further, the compared systems showed different responses in terms of effectiveness in reducing the discharged mass to the RWB in relation to the particular pollutant taken into account.

Water flow in carbon-based nanoporous membranes impacted by interactions between hydrated ions and aromatic rings.

PubMed

Liu, Jian; Shi, Guosheng; Fang, Haiping

2017-02-24

Carbon-based nanoporous membranes, such as carbon nanotubes (CNTs), graphene/graphene oxide and graphyne, have shown great potential in water desalination and purification, gas and ion separation, biosensors, and lithium-based batteries, etc. A deep understanding of the interaction between hydrated ions in an aqueous solution and the graphitic surface in systems composed of water, ions and a graphitic surface is essential for applications with carbon-based nanoporous membrane platforms. In this review, we describe the recent progress of the interaction between hydrated ions and aromatic ring structures on the carbon-based surface and its applications in the water flow in a carbon nanotube. We expect that these works can be extended to the understanding of water flow in other nanoporous membranes, such as nanoporous graphene, graphyne and stacked sheets of graphene oxide.

Quantitative Magnetic Separation of Particles and Cells Using Gradient Magnetic Ratcheting.

PubMed

Murray, Coleman; Pao, Edward; Tseng, Peter; Aftab, Shayan; Kulkarni, Rajan; Rettig, Matthew; Di Carlo, Dino

2016-04-13

Extraction of rare target cells from biosamples is enabling for life science research. Traditional rare cell separation techniques, such as magnetic activated cell sorting, are robust but perform coarse, qualitative separations based on surface antigen expression. A quantitative magnetic separation technology is reported using high-force magnetic ratcheting over arrays of magnetically soft micropillars with gradient spacing, and the system is used to separate and concentrate magnetic beads based on iron oxide content (IOC) and cells based on surface expression. The system consists of a microchip of permalloy micropillar arrays with increasing lateral pitch and a mechatronic device to generate a cycling magnetic field. Particles with higher IOC separate and equilibrate along the miropillar array at larger pitches. A semi-analytical model is developed that predicts behavior for particles and cells. Using the system, LNCaP cells are separated based on the bound quantity of 1 μm anti-epithelial cell adhesion molecule (EpCAM) particles as a metric for expression. The ratcheting cytometry system is able to resolve a ±13 bound particle differential, successfully distinguishing LNCaP from PC3 populations based on EpCAM expression, correlating with flow cytometry analysis. As a proof-of-concept, EpCAM-labeled cells from patient blood are isolated with 74% purity, demonstrating potential toward a quantitative magnetic separation instrument. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Suppression of turbulent energy cascade due to phase separation in homogenous binary mixture fluid

NASA Astrophysics Data System (ADS)

Takagi, Youhei; Okamoto, Sachiya

2015-11-01

When a multi-component fluid mixture becomes themophysically unstable state by quenching from well-melting condition, phase separation due to spinodal decomposition occurs, and a self-organized structure is formed. During phase separation, free energy is consumed for the structure formation. In our previous report, the phase separation in homogenous turbulence was numerically simulated and the coarsening process of phase separation was discussed. In this study, we extended our numerical model to a high Schmidt number fluid corresponding to actual polymer solution. The governing equations were continuity, Navier-Stokes, and Chan-Hiliard equations as same as our previous report. The flow filed was an isotropic homogenous turbulence, and the dimensionless parameters in the Chan-Hilliard equation were estimated based on the thermophysical condition of binary mixture. From the numerical results, it was found that turbulent energy cascade was drastically suppressed in the inertial subrange by phase separation for the high Schmidt number flow. By using the identification of turbulent and phase separation structure, we discussed the relation between total energy balance and the structures formation processes. This study is financially supported by the Grand-in-Aid for Young Scientists (B) (No. T26820045) from the Ministry of Education, Cul-ture, Sports, Science and Technology of Japan.

High efficiency virtual impactor

DOEpatents

Loo, B.W.

1980-03-27

Environmental monitoring of atmospheric air is facilitated by a single stage virtual impactor for separating an inlet flow (Q/sub 0/) having particulate contaminants into a coarse particle flow (Q/sub 1/) and a fine particle flow (Q/sub 2/) to enable collection of such particles on different filters for separate analysis. An inlet particle acceleration nozzle and coarse particle collection probe member having a virtual impaction opening are aligned along a single axis and spaced apart to define a flow separation region at which the fine particle flow (Q/sub 2/) is drawn radially outward into a chamber while the coarse particle flow (Q/sub 1/) enters the virtual impaction opening.

Minnowbrook VI: 2009 Workshop on Flow Physics and Control for Internal and External Aerodynamics

NASA Technical Reports Server (NTRS)

LaGraff, John E.; Povinelli, Louis A.; Gostelow, J. Paul; Glauser, Mark

2010-01-01

Topics covered include: Flow Physics and control for Internal and External Aerodynamics (not in TOC...starts on pg13); Breaking CFD Bottlenecks in Gas-Turbine Flow-Path Design; Streamwise Vortices on the Convex Surfaces of Circular Cylinders and Turbomachinery Blading; DNS and Embedded DNS as Tools for Investigating Unsteady Heat Transfer Phenomena in Turbines; Cavitation, Flow Structure and Turbulence in the Tip Region of a Rotor Blade; Development and Application of Plasma Actuators for Active Control of High-Speed and High Reynolds Number Flows; Active Flow Control of Lifting Surface With Flap-Current Activities and Future Directions; Closed-Loop Control of Vortex Formation in Separated Flows; Global Instability on Laminar Separation Bubbles-Revisited; Very Large-Scale Motions in Smooth and Rough Wall Boundary Layers; Instability of a Supersonic Boundary-Layer With Localized Roughness; Active Control of Open Cavities; Amplitude Scaling of Active Separation Control; U.S. Air Force Research Laboratory's Need for Flow Physics and Control With Applications Involving Aero-Optics and Weapon Bay Cavities; Some Issues Related to Integrating Active Flow Control With Flight Control; Active Flow Control Strategies Using Surface Pressure Measurements; Reduction of Unsteady Forcing in a Vaned, Contra-Rotating Transonic Turbine Configuration; Active Flow Control Stator With Coanda Surface; Controlling Separation in Turbomachines; Flow Control on Low-Pressure Turbine Airfoils Using Vortex Generator Jets; Reduced Order Modeling Incompressible Flows; Study and Control of Flow Past Disk, and Circular and Rectangular Cylinders Aligned in the Flow; Periodic Forcing of a Turbulent Axisymmetric Wake; Control of Vortex Breakdown in Critical Swirl Regime Using Azimuthal Forcing; External and Turbomachinery Flow Control Working Group; Boundary Layers, Transitions and Separation; Efficiency Considerations in Low Pressure Turbines; Summary of Conference; and Final Plenary Session Transcript.

Reversible chemical delithiation/lithiation of LiFePO4: towards a redox flow lithium-ion battery.

PubMed

Huang, Qizhao; Li, Hong; Grätzel, Michael; Wang, Qing

2013-02-14

Reversible chemical delithiation/lithiation of LiFePO(4) was successfully demonstrated using ferrocene derivatives, based on which a novel energy storage system--the redox flow lithium-ion battery (RFLB), was devised by integrating the operation flexibility of a redox flow battery and high energy density of a lithium-ion battery. Distinct from the recent semi-solid lithium rechargeable flow battery, the energy storage materials of RFLB stored in separate energy tanks remain stationary upon operation, giving us a fresh perspective on building large-scale energy storage systems with higher energy density and improved safety.

Vortical structures of supersonic flow over a delta-wing on a flat plate

NASA Astrophysics Data System (ADS)

Wang, D. P.; Xia, Z. X.; Zhao, Y. X.; Wang, Q. H.; Liu, B.

2013-02-01

Employing the nanoparticle-based planar laser scattering (NPLS), supersonic flow over a delta-winged vortex generator on a flat plate was experimentally investigated in a supersonic quiet wind tunnel at Ma = 2.68. The fine structures of the flow field, shock waves, separation vortices, wake, and boundary layer transition were observed in the NPLS images. According to the time-correlation of the NPLS images and the measurement results of particle image velocimetry, the structural model of the flow field was improved further, and coherent wake structures were observed, which is of significance theoretically and in engineering application.

Coupling Flash LC with MS for enrichment and isolation of milk oligosaccharides for functional studies

PubMed Central

Strum, John S.; Aldredge, Danielle; Barile, Daniela; Lebrilla, Carlito B.

2013-01-01

Mass spectrometry has been coupled with flash liquid chromatography to yield new capabilities for isolating non-chromophoric material from complicated biological mixtures. A flash LC/MS/MS method enabled fraction collection of milk oligosaccharides from biological mixtures based on composition and structure. The method is compatible with traditional gas-pressure driven flow flash chromatography, widely employed in organic chemistry laboratories. The on-line mass detector enabled real-time optimization of chromatographic parameters to favor separation of oligosaccharides that would otherwise be indistinguishable from co-eluting components with a non-specific detector. Unlike previously described preparative LC/MS techniques, we have employed a dynamic flow connection that permits any flow rate from the flash system to be delivered from 1–200 mL/min without affecting the ionization conditions of the mass spectrometer. A new way of packing large amounts of graphitized carbon allowed the enrichment and separation of milligram quantities of structurally heterogeneous mixtures of human milk oligosaccharides (HMOs) and bovine milk oligosaccharides (BMOs). Abundant saccharide components in milk, such as lactose and lacto-N-tetraose, were separated from the rarer and less abundant oligosaccharides that have greater structural diversity and biological functionality. Neutral and acidic HMOs and BMOs were largely separated and enriched with a dual binary solvent system. PMID:22370281

Separation system with a sheath-flow supported electrochemical detector

DOEpatents

Mathies, Richard A [Moraga, CA; Emrich, Charles A [Berkeley, CA; Singhal, Pankaj [Pasadena, CA; Ertl, Peter [Styria, AT

2008-10-21

An electrochemical detector including side channels associated with a separation channel of a sample component separation apparatus is provided. The side channels of the detector, in one configuration, provide a sheath-flow for an analyte exiting the separation channel which directs the analyte to the electrically developed electrochemical detector.

A New Reynolds Stress Algebraic Equation Model

NASA Technical Reports Server (NTRS)

Shih, Tsan-Hsing; Zhu, Jiang; Lumley, John L.

1994-01-01

A general turbulent constitutive relation is directly applied to propose a new Reynolds stress algebraic equation model. In the development of this model, the constraints based on rapid distortion theory and realizability (i.e. the positivity of the normal Reynolds stresses and the Schwarz' inequality between turbulent velocity correlations) are imposed. Model coefficients are calibrated using well-studied basic flows such as homogeneous shear flow and the surface flow in the inertial sublayer. The performance of this model is then tested in complex turbulent flows including the separated flow over a backward-facing step and the flow in a confined jet. The calculation results are encouraging and point to the success of the present model in modeling turbulent flows with complex geometries.

Active control of massively separated high-speed/base flows with electric arc plasma actuators

NASA Astrophysics Data System (ADS)

DeBlauw, Bradley G.

The current project was undertaken to evaluate the effects of electric arc plasma actuators on high-speed separated flows. Two underlying goals motivated these experiments. The first goal was to provide a flow control technique that will result in enhanced flight performance for supersonic vehicles by altering the near-wake characteristics. The second goal was to gain a broader and more sophisticated understanding of these complex, supersonic, massively-separated, compressible, and turbulent flow fields. The attainment of the proposed objectives was facilitated through energy deposition from multiple electric-arc plasma discharges near the base corner separation point. The control authority of electric arc plasma actuators on a supersonic axisymmetric base flow was evaluated for several actuator geometries, frequencies, forcing modes, duty cycles/on-times, and currents. Initially, an electric arc plasma actuator power supply and control system were constructed to generate the arcs. Experiments were performed to evaluate the operational characteristics, electromagnetic emission, and fluidic effect of the actuators in quiescent ambient air. The maximum velocity induced by the arc when formed in a 5 mm x 1.6 mm x 2 mm deep cavity was about 40 m/s. During breakdown, the electromagnetic emission exhibited a rise and fall in intensity over a period of about 340 ns. After breakdown, the emission stabilized to a near-constant distribution. It was also observed that the plasma formed into two different modes: "high-voltage" and "low-voltage". It is believed that the plasma may be switching between an arc discharge and a glow discharge for these different modes. The two types of plasma do not appear to cause substantial differences on the induced fluidic effects of the actuator. In general, the characterization study provided a greater fundamental understanding of the operation of the actuators, as well as data for computational model comparison. Preliminary investigations of actuator geometry in the supersonic base flow determined that inclined cavity and normal cavity actuators positioned on the base near the base edge could produce significant disturbances in the shear layer. The disturbances were able to be tracked in time with phase-locked schlieren imaging and particle image velocimetry (PIV). The final set of flow control experiments were therefore performed with an eight-actuator base using the inclined cavity actuator geometry. The actuators were able to cause moderate influences on the axisymmetric shear layer velocity profile and base pressure. The most substantial changes to the shear layer and base pressure were noted for the highest current and duty cycle tests. At 1 A and 20% duty cycle, the base pressure was reduced by 3.5%. Similar changes were noted for all modes and a range of frequencies from about 10-30 kHz. Increases in duty cycle between 4% and 20% caused a nearly linear decrease in base pressure. Analysis of the shear layer velocity profiles acquired through PIV show a local thickening of the shear layer in the region of the disturbances caused by the actuator. A slight increase in thickness was also observed away from the disturbance. Disturbances were able to be tracked at all frequencies and translated along the shear layer at a convective velocity of 430 +/- 20 m/s. A fairly clear trend of increasing velocity disturbance amplitude correlating to increasing base pressure changes was noted. Moreover, the ability of the disturbances to stay well organized further down the shear layer also appears to be a significant factor in the actuators' effect on base pressure. Consistent with these observations, it appears that increased duty cycle causes increased shear layer disturbance amplitudes. The use of PIV has enabled substantial insight to be gained into the effects of the actuators on the ensemble-averaged flow field and on the variability of the instantaneous flow field with and without control. A sensitive bimodal recirculation region behavior was found in the no-control flow field that the plasma actuators could force. The flow field and turbulence statistics in each mode were substantially different. Through analysis of past no-control base pressure measurements, it is believed that the bimodal behavior fluctuates at a characteristic frequency between 0.4 and 0.5 Hz [StD = [special character omitted](5x10-5)]. The flat time-averaged base pressure distribution is due to the superposition of a normally non-flat instantaneous base pressure distribution. Also, the standard deviation of the base pressure measurements is reduced when in one recirculation region mode as compared to when it is fluctuating between recirculation region modes.

Sweep and Compressibility Effects on Active Separation Control at High Reynolds Numbers

NASA Technical Reports Server (NTRS)

Seifert, Avi; Pack, LaTunia G.

2000-01-01

This paper explores the effects of compressibility, sweep and excitation location on active separation control at high Reynolds numbers. The model, which was tested in a cryogenic pressurized wind tunnel, simulates the upper surface of a 20% thick GlauertGoldschmied type airfoil at zero angle of attack. The flow is fully turbulent since the tunnel sidewall boundary layer flows over the model. Without control, the flow separates at the highly convex area and a large turbulent separation bubble is formed. Periodic excitation is applied to gradually eliminate the separation bubble. Two alternative blowing slot locations as well as the effect of compressibility, sweep and steady suction or blowing were studied. During the test the Reynolds numbers ranged from 2 to 40 million and Mach numbers ranged from 0.2 to 0.7. Sweep angles were 0 and 30 deg. It was found that excitation must be introduced slightly upstream of the separation region regardless of the sweep angle at low Mach number. Introduction of excitation upstream of the shock wave is more effective than at its foot. Compressibility reduces the ability of steady mass transfer and periodic excitation to control the separation bubble but excitation has an effect on the integral parameters, which is similar to that observed in low Mach numbers. The conventional swept flow scaling is valid for fully and even partially attached flow, but different scaling is required for the separated 3D flow. The effectiveness of the active control is not reduced by sweep. Detailed flow field dynamics are described in the accompanying paper.

Sweep and Compressibility Effects on Active Separation Control at High Reynolds Numbers

NASA Technical Reports Server (NTRS)

Seifert, Avi; Pack, LaTunia G.

2000-01-01

This paper explores the effects of compressibility, sweep and excitation location on active separation control at high Reynolds numbers. The model, which was tested in a cryogenic pressurized wind tunnel, simulates the upper surface of a 20% thick Glauert Goldschmied type airfoil at zero angle of attack. The flow is fully turbulent since the tunnel sidewall boundary layer flows over the model. Without control, the flow separates at the highly convex area and a large turbulent separation bubble is formed. Periodic excitation is applied to gradually eliminate the separation bubble. Two alternative blowing slot locations as well as the effect of compressibility, sweep and steady suction or blowing were studied. During the test the Reynolds numbers ranged from 2 to 40 million and Mach numbers ranged from 0.2 to 0.7. Sweep angles were 0 and 30 deg. It was found that excitation must be introduced slightly upstream of the separation region regardless of the sweep angle at low Mach number. Introduction of excitation upstream of the shock wave is more effective than at its foot. Compressibility reduces the ability of steady mass transfer and periodic excitation to control the separation bubble but excitation has an effect on the integral parameters, which is similar to that observed in low Mach numbers. The conventional swept flow scaling is valid for fully and even partially attached flow, but different scaling is required for the separated 3D flow. The effectiveness of the active control is not reduced by sweep. Detailed flow field dynamics are described in the accompanying paper.

Numerical and experimental investigation of VG flow control for a low-boom inlet

NASA Astrophysics Data System (ADS)

Rybalko, Michael

The application of vortex generators (VGs) for shock/boundary layer interaction flow control in a novel external compression, axisymmetric, low-boom concept inlet was studied using numerical and experimental methods. The low-boom inlet design features a zero-angle cowl and relaxed isentropic compression centerbody spike, resulting in defocused oblique shocks and a weak terminating normal shock. This allows reduced external gas dynamic waves at high mass flow rates but suffers from flow separation near the throat and a large hub-side boundary layer at the Aerodynamic Interface Plane (AIP), which marks the inflow to the jet engine turbo-machinery. Supersonic VGs were investigated to reduce the shock-induced flow separation near the throat while subsonic VGs were investigated to reduce boundary layer radial distortion at the AIP. To guide large-scale inlet experiments, Reynolds-Averaged Navier-Stokes (RANS) simulations using three-dimensional, structured, chimera (overset) grids and the WIND-US code were conducted. Flow control cases included conventional and novel types of vortex generators at positions both upstream of the terminating normal shock (supersonic VGs) and downstream (subsonic VGs). The performance parameters included incompressible axisymmetric shape factor, post-shock separation area, inlet pressure recovery, and mass flow ratio. The design of experiments (DOE) methodology was used to select device size and location, analyze the resulting data, and determine the optimal choice of device geometry. Based on the above studies, a test matrix of supersonic and subsonic VGs was adapted for a large-scale inlet test to be conducted at the 8'x6' supersonic wind tunnel at NASA Glenn Research Center (GRC). Comparisons of RANS simulations with data from the Fall 2010 8'x6' inlet test showed that predicted VG performance trends and case rankings for both supersonic and subsonic devices were consistent with experimental results. For example, experimental surface oil flow visualization revealed a significant post-shock separation bubble with flow recirculation for the baseline (no VG) case that was substantially broken up in the micro-ramp VG case, consistent with simulations. Furthermore, the predicted subsonic VG performance with respect to a reduction in radial distortion (quantified in terms of axisymmetric incompressible shape factor) was found to be consistent with boundary layer rake measurements. To investigate the unsteady turbulent flow features associated with the shock-induced flow separation and the hub-side boundary layer, a detached eddy simulation (DES) approach using the WIND-US code was employed to model the baseline inlet flow field. This approach yielded improved agreement with experimental data for time-averaged diffuser stagnation pressure profiles and allowed insight into the pressure fluctuations and turbulent kinetic energy distributions which may be present at the AIP. In addition, streamwise shock position statistics were obtained and compared with experimental Schlieren results. The predicted shock oscillations were much weaker than those seen experimentally (by a factor of four), which indicates that the mechanism for the experimental shock oscillations was not captured. In addition, the novel supersonic vortex generator geometries were investigated experimentally (prior to the large-scale inlet 8'x6' wind tunnel tests) in an inlet-relevant flow field containing a Mach 1.4 normal shock wave followed by a subsonic diffuser. A parametric study of device height and distance upstream of the normal shock was undertaken for split-ramp and ramped-vane geometries. Flow field diagnostics included high-speed Schlieren, oil flow visualization, and Pitot-static pressure measurements. Parameters including flow separation, pressure recovery, centerline incompressible boundary layer shape factor, and shock stability were analyzed and compared to the baseline uncontrolled case. While all vortex generators tested eliminated centerline flow separation, the presence of VGs also increased the significant three-dimensionality of the flow via increased side-wall interaction. The stronger streamwise vorticity generated by ramped-vanes also yielded improved pressure recovery and fuller boundary layer velocity profiles within the subsonic diffuser. (Abstract shortened by UMI.)

User's guide for ENSAERO: A multidisciplinary program for fluid/structural/control interaction studies of aircraft (release 1)

NASA Technical Reports Server (NTRS)

Guruswamy, Guru P.

1994-01-01

Strong interactions can occur between the flow about an aerospace vehicle and its structural components resulting in several important aeroelastic phenomena. These aeroelastic phenomena can significantly influence the performance of the vehicle. At present, closed-form solutions are available for aeroelastic computations when flows are in either the linear subsonic or supersonic range. However, for aeroelasticity involving complex nonlinear flows with shock waves, vortices, flow separations, and aerodynamic heating, computational methods are still under development. These complex aeroelastic interactions can be dangerous and limit the performance of aircraft. Examples of these detrimental effects are aircraft with highly swept wings experiencing vortex-induced aeroelastic oscillations, transonic regime at which the flutter speed is low, aerothermoelastic loads that play a critical role in the design of high-speed vehicles, and flow separations that often lead to buffeting with undesirable structural oscillations. The simulation of these complex aeroelastic phenomena requires an integrated analysis of fluids and structures. This report presents a summary of the development, applications, and procedures to use the multidisciplinary computer code ENSAERO. This code is based on the Euler/Navier-Stokes flow equations and modal/finite-element structural equations.

Investigation of Positively Curved Blade in Compressor Cascade Based on Transition Model

NASA Astrophysics Data System (ADS)

Chen, Shaowen; Lan, Yunhe; Zhou, Zhihua; Wang, Songtao

2016-06-01

Experiment and numerical simulation of flow transition in a compressor cascade with positively curved blade is carried out in a low speed. In the experimental investigation, the outlet aerodynamic parameters are measured using a five-hole aerodynamic probe, and an ink-trace flow visualization is applied to the cascade surface. The effects of transition flow on the boundary layer development, three-dimensional flow separation and aerodynamic performance are studied. The feasibility of a commercial computational fluid dynamic code is validated and the numerical results show a good agreement with experimental data. The blade-positive curving intensifies the radial force from the endwalls to the mid-span near the suction surface, which leads to the smaller scope of the intermittent region, the lesser extents of turbulence intensity and the shorter radial height of the separation bubble near the endwalls, but has little influence on the flow near the mid-span. The large passage vortex is divided into two smaller shedding vortexes under the impact of the radial pressure gradient due to the positively curved blade. The new concentrated shedding vortex results in an increase in the turbulence intensity and secondary flow loss of the corresponding region.

An analysis method for multi-component airfoils in separated flow

NASA Technical Reports Server (NTRS)

Rao, B. M.; Duorak, F. A.; Maskew, B.

1980-01-01

The multi-component airfoil program (Langley-MCARF) for attached flow is modified to accept the free vortex sheet separation-flow model program (Analytical Methods, Inc.-CLMAX). The viscous effects are incorporated into the calculation by representing the boundary layer displacement thickness with an appropriate source distribution. The separation flow model incorporated into MCARF was applied to single component airfoils. Calculated pressure distributions for angles of attack up to the stall are in close agreement with experimental measurements. Even at higher angles of attack beyond the stall, correct trends of separation, decrease in lift coefficients, and increase in pitching moment coefficients are predicted.

The efficient simulation of separated three-dimensional viscous flows using the boundary-layer equations

NASA Technical Reports Server (NTRS)

Van Dalsem, W. R.; Steger, J. L.

1985-01-01

A simple and computationally efficient algorithm for solving the unsteady three-dimensional boundary-layer equations in the time-accurate or relaxation mode is presented. Results of the new algorithm are shown to be in quantitative agreement with detailed experimental data for flow over a swept infinite wing. The separated flow over a 6:1 ellipsoid at angle of attack, and the transonic flow over a finite-wing with shock-induced 'mushroom' separation are also computed and compared with available experimental data. It is concluded that complex, separated, three-dimensional viscous layers can be economically and routinely computed using a time-relaxation boundary-layer algorithm.

3D CFD simulation of Multi-phase flow separators

NASA Astrophysics Data System (ADS)

Zhu, Zhiying

2017-10-01

During the exploitation of natural gas, some water and sands are contained. It will be better to separate water and sands from natural gas to insure favourable transportation and storage. In this study, we use CFD to analyse the effect of multi-phase flow separator, whose detailed geometrical parameters are designed in advanced. VOF model and DPM are used here. From the results of CFD, we can draw a conclusion that separated effect of multi-phase flow achieves better results. No solid and water is carried out from gas outlet. CFD simulation provides an economical and efficient approach to shed more light on details of the flow behaviour.

Incidence angle bounds for lip flow separation of three 13.97-centimeter-diameter inlets

NASA Technical Reports Server (NTRS)

Luidens, R. W.; Abbott, J. M.

1976-01-01

Low speed wind tunnel tests were conducted to establish a procedure for determining inlet-lip flow separation and to make preliminary examination of the incidence angle bounds for lip flow separation on inlets intended for the nacelles of STOL (short takeoff and landing) aircraft. Three inlets were tested. Two of the inlets had short centerbodies with lower lip area contraction ratios of 1.30 and 1.44. The third inlet had a cylindrical centerbody extended forward into the inlet throat with a lower lip area contraction ratio of 1.44. The inlets were sized to fit a 13.97 centimeter-diameter fan. For inlet throat Mach numbers less than about 0.43, the lip flow separation angle was increased by either increasing the ratio of throat velocity to freestream velocity (Vt/Vo) or by increasing the lower lip area contraction ratio. For throat Mach numbers greater than a certain value (ranging from 0.43 to 0.52), increasing throat Mach number in some cases resulted in a decrease in the lip flow separation angle. Extending a cylindrical centerbody into the inlet throat increased the flow separation angle for nearly all values of Vt/Vo.

Vortex-induced dynamic loads on a non-spinning volleyball

NASA Astrophysics Data System (ADS)

Qing-ding, Wei; Rong-sheng, Lin; Zhi-jie, Liu

1988-09-01

An experiment on vortex-induced dynamic loads on a non-spinning Volleyball was conducted in a wind tunnel. The flow past the Volleyball was visualized, and the aerodynamic load was measured by use of a strain gauge balance. The separation on the Volleyball was measured with hot-film. The experimental results suggest that under the action of an unstable tail vortex system the separation region is changeable, and that the fluctuation of drag and lateral forces is the same order of magnitude as the mean drag, no matter whether the seam of the Volleyball is symmetric or asymmetric, with regard to the flow. Based on the experimental data a numerical simulation of Volleyball swerve motion was made.

Efficient gas-separation process to upgrade dilute methane stream for use as fuel

DOEpatents

Wijmans, Johannes G [Menlo Park, CA; Merkel, Timothy C [Menlo Park, CA; Lin, Haiqing [Mountain View, CA; Thompson, Scott [Brecksville, OH; Daniels, Ramin [San Jose, CA

2012-03-06

A membrane-based gas separation process for treating gas streams that contain methane in low concentrations. The invention involves flowing the stream to be treated across the feed side of a membrane and flowing a sweep gas stream, usually air, across the permeate side. Carbon dioxide permeates the membrane preferentially and is picked up in the sweep air stream on the permeate side; oxygen permeates in the other direction and is picked up in the methane-containing stream. The resulting residue stream is enriched in methane as well as oxygen and has an EMC value enabling it to be either flared or combusted by mixing with ordinary air.

Particle-laden swirling free jets: Measurements and predictions

NASA Technical Reports Server (NTRS)

Bulzan, D. L.; Shuen, J.-S.; Faeth, G. M.

1987-01-01

A theoretical and experimental investigation of single-phase and particle-laden weakly swirling jets was conducted. The jets were injected vertically downward from a 19 mm diameter tube with swirl numbers ranging from 0 to 0.33. The particle-laden jets had a single loading ratio (0.2) with particles having a SMD of 39 microns. Mean and fluctuating properties of both phases were measured using nonintrusive laser based methods while particle mass flux was measured using an isokinetic sampling probe. The continuous phase was analyzed using both a baseline kappa-epsilon turbulence model and an extended version with modifications based on the flux Richardson number to account for effects of streamline curvature. To highlight effects of interphase transport rates and particle/turbulence interactions, effects of the particles were analyzed as follows: (1) locally homogeneous flow (LHF) analysis, where interphase transport rates are assumed to be infinitely fast; (2) deterministic separated flow (DSF) analysis, where finite interphase transport rates are considered but particle/turbulence interactions are ignored; and (3) stochastic separated flow (SSF) analysis, where both effects are considered using random-walk computations.

Flow chemistry and polymer-supported pseudoenantiomeric acylating agents enable parallel kinetic resolution of chiral saturated N-heterocycles

NASA Astrophysics Data System (ADS)

Kreituss, Imants; Bode, Jeffrey W.

2017-05-01

Kinetic resolution is a common method to obtain enantioenriched material from a racemic mixture. This process will deliver enantiopure unreacted material when the selectivity factor of the process, s, is greater than 1; however, the scalemic reaction product is often discarded. Parallel kinetic resolution, on the other hand, provides access to two enantioenriched products from a single racemic starting material, but suffers from a variety of practical challenges regarding experimental design that limit its applications. Here, we describe the development of a flow-based system that enables practical parallel kinetic resolution of saturated N-heterocycles. This process provides access to both enantiomers of the starting material in good yield and high enantiopurity; similar results with classical kinetic resolution would require selectivity factors in the range of s = 100. To achieve this, two immobilized quasienantiomeric acylating agents were designed for the asymmetric acylation of racemic saturated N-heterocycles. Using the flow-based system we could efficiently separate, recover and reuse the polymer-supported reagents. The amide products could be readily separated and hydrolysed to the corresponding amines without detectable epimerization.

High speed Deans switch for low duty cycle comprehensive two-dimensional gas chromatography.

PubMed

Ghosh, Abhijit; Bates, Carly T; Seeley, Stacy K; Seeley, John V

2013-05-24

A new high-speed valve-based modulator has been designed and tested for use in comprehensive two-dimensional gas chromatography (GC×GC). The modulator is a Deans switch constructed from two micro-volume fittings and a solenoid valve. Modulator performance was characterized over a wide range of device settings including the magnitude of the switching flow, the gap between the tips of the primary and secondary column, the primary column flow rate, and the carrier gas identity. Under optimized conditions, the modulator was found to be capable of generating narrow pulses (<50ms) of primary effluent with a 2mL/min secondary column flow. This capability will ultimately allow the modulator to be used with GC×GC separations employing a wide range of detectors and secondary column geometries. The main disadvantage of this modulator is that it employs a low sampling duty cycle, and thus it produces separations with sensitivities that are lower than those produced with thermal modulators or differential flow modulators. The efficacy of the new high-speed Deans switch modulator was demonstrated through the GC×GC separation of a hydrocarbon standard and gasoline. Precise quantitation of individual components was possible provided the modulation ratio was kept greater than 2.0. Copyright © 2013 Elsevier B.V. All rights reserved.

Control of a Normal Shock Boundary Layer Interaction with Ramped Vanes of Various Sizes

NASA Astrophysics Data System (ADS)

Lee, Sang; Loth, Eric

2017-11-01

A novel vortex generator design positioned upstream of a normal shock and a subsequent diffuser was investigated using large eddy simulations. In particular, ``ramped-vane'' flow control devices with three difference heights relative to the incoming boundary layer thickness (0.34 δ 0.52 δ and 0.75 δ were placed in a supersonic boundary layer with a freestream Mach number of 1.3 and a Reynolds number of 2,400 based on momentum thickness. These devices are similar to subsonic vanes but are designed to be more mechanically robust while having low wave drag. The devices generated strong streamwise vortices that entrained high momentum fluid to the near-wall region and increased turbulent mixing. The devices also decreased shock-induced flow separation, which resulted in a higher downstream skin friction in the diffuser. In general, the largest ramped-vane (0.75 δ) produced the largest reductions in flow separation, shape factor and overall unsteadiness. However, the medium-sized ramped vane (0.52 δ) was able to also reduce both the separation area and the diffuser displacement thickness. The smallest device (0.34 δ) had a weak impact of the flow in the diffuser, though a 10% reduction in the shape factor was achieved.

Microgravity Segregation in Binary Mixtures of Inelastic Spheres Driven by Velocity Fluctuation Gradients

NASA Technical Reports Server (NTRS)

Jenkins, James T.; Louge, Michel Y.

1996-01-01

We are interested in collisional granular flows of dry materials in reduced gravity. Because the particles interact through collisions, the energy of the particle velocity fluctuations plays an important role in the physics. Here we focus on the separation of grains by properties - size, for example - that is driven by spatial gradients in the fluctuation energy of the grains. The segregation of grains by size is commonly observed in geophysical flows and industrial processes. Segregation of flowing grains can also take place based on other properties, e.g. shape, mass, friction, and coefficient of restitution. Many mechanisms may be responsible for segregation; most of these are strongly influenced by gravity. Here, we outline a mechanism that is independent of gravity. This mechanism may be important but is often obscured in terrestrial grain flows. It is driven by gradients in fluctuation energy. In microgravity, the separation of grains by property will proceed slowly enough to permit flight observations to provide an unambiguous measurement of the transport coefficients associated with the segregation. In this context, we are planning a microgravity shear cell experiment that contains a mixture of two types of spherical grains. The grains will be driven to interact with two different types of boundaries on either sides of the cell. The resulting separation will be observed visually.

Autonomous Agent-Based Systems and Their Applications in Fluid Dynamics, Particle Separation, and Co-evolving Networks

NASA Astrophysics Data System (ADS)

Graeser, Oliver

This thesis comprises three parts, reporting research results in Fluid Dynamics (Part I), Particle Separation (Part II) and Co-evolving Networks (Part III). Part I deals with the simulation of fluid dynamics using the lattice-Boltzmann method. Microfluidic devices often feature two-dimensional, repetitive arrays. Flows through such devices are pressure-driven and confined by solid walls. We have defined new adaptive generalised periodic boundary conditions to represent the effects of outer solid walls, and are thus able to exploit the periodicity of the array by simulating the flow through one unit cell in lieu of the entire device. The so-calculated fully developed flow describes the flow through the entire array accurately, but with computational requirements that are reduced according to the dimensions of the array. Part II discusses the problem of separating macromolecules like proteins or DNA coils. The reliable separation of such molecules is a crucial task in molecular biology. The use of Brownian ratchets as mechanisms for the separation of such particles has been proposed and discussed during the last decade. Pressure-driven flows have so far been dismissed as possible driving forces for Brownian ratchets, as they do not generate ratchet asymmetry. We propose a microfluidic design that uses pressure-driven flows to create asymmetry and hence allows particle separation. The dependence of the asymmetry on various factors of the microfluidic geometry is discussed. We further exemplify the feasibility of our approach using Brownian dynamics simulations of particles of different sizes in such a device. The results show that ratchet-based particle separation using flows as the driving force is possible. Simulation results and ratchet theory predictions are in excellent agreement. Part III deals with the co-evolution of networks and dynamic models. A group of agents occupies the nodes of a network, which defines the relationship between these agents. The evolution of the agents is defined by the rules of the dynamic model and depends on the relationship between agents, i.e., the state of the network. In return, the evolution of the network depends on the state of the dynamic model. The concept is introduced through the adaptive SIS model. We show that the previously used criterion determining the critical infected fraction, i.e., the number of infected agents required to sustain the epidemic, is inappropriate for this model. We introduce a different criterion and show that the critical infected fraction so determined is in good agreement with results obtained by numerical simulations. We further discuss the concept of co-evolving dynamics using the Snowdrift Game as a model paradigm. Co-evolution occurs through agents cutting dissatisfied links and rewiring to other agents at random. The effect of co-evolution on the emergence of cooperation is discussed using a mean-field theory and numerical simulations. A transition between a connected and a disconnected, highly cooperative state of the system is observed, and explained using the mean-field model. Quantitative deviations regarding the level of cooperation in the disconnected regime can be fully resolved through an improved mean-field theory that includes the effect of random fluctuations into its model.

Computation of subsonic flow around airfoil systems with multiple separation

NASA Technical Reports Server (NTRS)

Jacob, K.

1982-01-01

A numerical method for computing the subsonic flow around multi-element airfoil systems was developed, allowing for flow separation at one or more elements. Besides multiple rear separation also sort bubbles on the upper surface and cove bubbles can approximately be taken into account. Also, compressibility effects for pure subsonic flow are approximately accounted for. After presentation the method is applied to several examples and improved in some details. Finally, the present limitations and desirable extensions are discussed.

Single cell Enrichment with High Throughput Microfluidic Devices

NASA Astrophysics Data System (ADS)

Pakjesm Pourfard, Pedram

Microfluidics is a rapidly growing field of biomedical engineering with numerous applications such as diagnostic testing, therapeutics, and research preparation. Cell enrichment for automated diagnostic is often assayed through measurement of biochemical and biophysical markers. Although biochemical markers have been widely used, intrinsic biophysical markers, such as, Shear migration, Lift force, Dean force, and many other label-free techniques, are advantageous since they don't require costly labeling or sample preparation. However, current passive techniques for enrichment had limited adoption in clinical and cell biology research applications. They generally require low flow rate and low cell volume fraction for high efficiency. The Control increment filtration, T-shaped microfluidic device, and spiral-shaped microfluidic devices will be studied for single-cell separation from aggregates. Control increment filtration works like the tangential filter; however, cells are separated based off of same amount of flow rate passing through large space gaps. Main microchannel of T-Shaped is connected to two perpendicular side channels. Based off Shear-modulated inertial migration, this device will enable selective enrichment of cells. The spiral shaped microfluidic device depends on different Dean and lift forces acting on cells to separate them based off different sizes. The spiral geometry of the microchannel will enable dominant inertial forces and the Dean Rotation force to cause larger cells to migrate to the inner side of the microchannel. Because manipulation of microchannel dimensions correlates to the degree of cell separation, versatility in design exists. Cell mixture samples will contain cells of different sizes and therefore design strategies could be utilized to maximize the effectiveness of single-cell separation.

Kinetically driven self-assembly of a binary solute mixture with controlled phase separation via electro-hydrodynamic flow of corona discharge.

PubMed

Jung, Hee Joon; Huh, June; Park, Cheolmin

2012-10-21

This feature article describes a new and facile process to fabricate a variety of thin films of non-volatile binary solute mixtures suitable for high performance organic electronic devices via electro-hydrodynamic flow of conventional corona discharge. Both Corona Discharge Coating (CDC) and a modified version of CDC, Scanning Corona Discharge Coating (SCDC), are based on utilizing directional electric flow, known as corona wind, of the charged uni-polar particles generated by corona discharge between a metallic needle and a bottom plate under a high electric field (5-10 kV cm(-1)). The electric flow rapidly spreads out the binary mixture solution on the bottom plate and subsequently forms a smooth and flat thin film in a large area within a few seconds. In the case of SCDC, the static movement of the bottom electrode on which a binary mixture solution is placed provides further control of thin film formation, giving rise to a film highly uniform over a large area. Interesting phase separation behaviors were observed including nanometer scale phase separation of a polymer-polymer binary mixture and vertical phase separation of a polymer-organic semiconductor mixture. Core-shell type phase separation of either polymer-polymer or polymer-colloidal nanoparticle binary mixtures was also developed with a periodically patterned microstructure when the relative location of the corona wind was controlled to a binary solution droplet on a substrate. We also demonstrate potential applications of thin functional films with controlled microstructures by corona coating to various organic electronic devices such as electroluminescent diodes, field effect transistors and non-volatile polymer memories.

Flow separation in a straight draft tube, particle image velocimetry

NASA Astrophysics Data System (ADS)

Duquesne, P.; Maciel, Y.; Ciocan, G. D.; Deschênes, C.

2014-03-01

As part of the BulbT project, led by the Consortium on Hydraulic Machines and the LAMH (Hydraulic Machine Laboratory of Laval University), the efficiency and power break off in a bulb turbine has been investigated. Previous investigations correlated the break off to draft tube losses. Tuft visualizations confirmed the emergence of a flow separation zone at the wall of the diffuser. Opening the guide vanes tends to extend the recirculation zone. The flow separations were investigated with two-dimensional and two-component particle image velocimetry (PIV) measurements designed based on the information collected from tuft visualizations. Investigations were done for a high opening blade angle with a N11 of 170 rpm, at best efficiency point and at two points with a higher Q11. The second operating point is inside the efficiency curve break off and the last operating point corresponds to a lower efficiency and a larger recirculation region in the draft tube. The PIV measurements were made near the wall with two cameras in order to capture two measurement planes simultaneously. The instantaneous velocity fields were acquired at eight different planes. Two planes located near the bottom wall were parallel to the generatrix of the conical part of the diffuser, while two other bottom planes diverged more from the draft tube axis than the cone generatrix. The last four planes were located on the draft tube side and diverged more from the draft tube axis than the cone generatrix. By combining the results from the various planes, the separation zone is characterized using pseudo-streamlines of the mean velocity fields, maps of the Reynolds stresses and maps of the reverse-flow parameter. The analysis provides an estimation of the separation zone size, shape and unsteady character, and their evolution with the guide vanes opening.

Kinetically driven self-assembly of a binary solute mixture with controlled phase separation via electro-hydrodynamic flow of corona discharge

NASA Astrophysics Data System (ADS)

Jung, Hee Joon; Huh, June; Park, Cheolmin

2012-09-01

This feature article describes a new and facile process to fabricate a variety of thin films of non-volatile binary solute mixtures suitable for high performance organic electronic devices via electro-hydrodynamic flow of conventional corona discharge. Both Corona Discharge Coating (CDC) and a modified version of CDC, Scanning Corona Discharge Coating (SCDC), are based on utilizing directional electric flow, known as corona wind, of the charged uni-polar particles generated by corona discharge between a metallic needle and a bottom plate under a high electric field (5-10 kV cm-1). The electric flow rapidly spreads out the binary mixture solution on the bottom plate and subsequently forms a smooth and flat thin film in a large area within a few seconds. In the case of SCDC, the static movement of the bottom electrode on which a binary mixture solution is placed provides further control of thin film formation, giving rise to a film highly uniform over a large area. Interesting phase separation behaviors were observed including nanometer scale phase separation of a polymer-polymer binary mixture and vertical phase separation of a polymer-organic semiconductor mixture. Core-shell type phase separation of either polymer-polymer or polymer-colloidal nanoparticle binary mixtures was also developed with a periodically patterned microstructure when the relative location of the corona wind was controlled to a binary solution droplet on a substrate. We also demonstrate potential applications of thin functional films with controlled microstructures by corona coating to various organic electronic devices such as electroluminescent diodes, field effect transistors and non-volatile polymer memories.

Photophoretic velocimetry for the characterization of aerosols.

PubMed

Haisch, Christoph; Kykal, Carsten; Niessner, Reinhard

2008-03-01

Aerosols are particles in a size range from some nanometers to some micrometers suspended in air or other gases. Their relevance varies as wide as their origin and composition. In the earth's atmosphere they influence the global radiation balance and human health. Artificially produced aerosols are applied, e.g., for drug administration, as paint and print pigments, or in rubber tire production. In all these fields, an exact characterization of single particles as well as of the particle ensemble is essential. Beyond characterization, continuous separation is often required. State-of-the-art separation techniques are based on electrical, thermal, or flow fields. In this work we present an approach to apply light in the form of photophoretic (PP) forces for characterization and separation of aerosol particles according to their optical properties. Such separation technique would allow, e.g., the separation of organic from inorganic particles of the same aerodynamic size. We present a system which automatically records velocities induced by PP forces and does a statistical evaluation in order to characterize the particle ensemble properties. The experimental system essentially consists of a flow cell with rectangular cross section (1 cm(2), length 7 cm), where the aerosol stream is pumped through in the vertical direction at ambient pressure. In the cell, a laser beam is directed orthogonally to the particle flow direction, which results in a lateral displacement of the particles. In an alternative configuration, the beam is directed in the opposite direction to the aerosol flow; hence, the particles are slowed down by the PP force. In any case, the photophoretically induced variations of speed and position are visualized by a second laser illumination and a camera system, feeding a mathematical particle tracking algorithm. The light source inducing the PP force is a diode laser (lambda = 806 nm, P = 0.5 W).

Evolution of engine cycles for STOVL propulsion concepts

NASA Technical Reports Server (NTRS)

Bucknell, R. L.; Frazier, R. H.; Giulianetti, D. J.

1990-01-01

Short Take-off, Vertical Landing (STOVL) demonstrator concepts using a common ATF engine core are discussed. These concepts include a separate fan and core flow engine cycle, mixed flow STOVL cycles, separate flow cycles convertible to mixed flow, and reaction control system engine air bleed. STOVL propulsion controls are discussed.

Hydrodynamic Assists Magnetophoreses Rare Cancer cells Separation in Microchannel Simulation and Experimental Verifications

NASA Astrophysics Data System (ADS)

Saeed, O.; Duru, L.; Yulin, D.

2018-05-01

A proposed microfluidic design has been fabricated and simulated using COMSOL Multiphysics software, based on two physical models included in this design. The device’s ability to create a narrow stream of the core sample by controlling the sheath flow rates Qs1 and Qs2 in both peripheral channels was investigated. The main target of this paper is to study the possibility of combing the hydrodynamic and magnetic techniques, in order to achieve a high rate of cancer cells separation from a cell mixture and/or buffer sample. The study has been conducted in two stages, firstly, the effects of the sheath flow rates (Qs1 and Qs2) on the sample stream focusing were studied, to find the proposed device effectiveness optimal conditions and its capability in cell focusing, and then the magnetic mechanism has been utilized to finalize the pre-labelled cells separation process.

Flow Separation Control Over a Ramp Using Sweeping Jet Actuators

NASA Technical Reports Server (NTRS)

Koklu, Mehti; Owens, Lewis R.

2014-01-01

Flow separation control on an adverse-pressure-gradient ramp model was investigated using various flow-control methods in the NASA Langley 15-Inch Wind Tunnel. The primary flow-control method studied used a sweeping jet actuator system to compare with more classic flow-control techniques such as micro-vortex generators, steady blowing, and steady- and unsteady-vortex generating jets. Surface pressure measurements and a new oilflow visualization technique were used to characterize the effects of these flow-control actuators. The sweeping jet actuators were run in three different modes to produce steady-straight, steady-angled, and unsteady-oscillating jets. It was observed that all of these flow-control methods are effective in controlling the separated flows on the ramp model. The steady-straight jet energizes the boundary layer by momentum addition and was found to be the least effective method for a fixed momentum coefficient. The steady-angled jets achieved better performance than the steady-straight jets because they generate streamwise vortices that energize the boundary layer by mixing high-momentum fluid with near wall low-momentum fluid. The unsteady-oscillating jets achieved the best performance by increasing the pressure recovery and reducing the downstream flow separation. Surface flow visualizations indicated that two out-of-phase counter-rotating vortices are generated per sweeping jet actuator, while one vortex is generated per vortex-generating jets. The extra vortex resulted in increased coverage, more pressure recovery, and reduced flow separation.

[Low-Frequency Flow Oscillation

NASA Technical Reports Server (NTRS)

Bragg, Michael B.

1997-01-01

The results of the research conducted under this grant are presented in detail in three Master theses, by Heinrich, Balow, and Broeren. Additional analysis of the experimental data can be found in two AIAA Journal articles and two conference papers. Citations for all of the studies' publications can be found in the bibliography which is attached. The objective of Heinrich's study was to document the low-frequency flow oscillation on the LRN-1007 airfoil, which had been previously observed at low Reynolds number, to determine its origin, and explore the phenomenon at higher Reynolds number. Heinrich performed detailed flow visualization on the airfoil using surface fluorescent oil and laser-sheet off-body visualization. A large leading-edge separation bubble and trailing-edge separation was identified on the airfoil just prior to the onset of the unsteady stall flow oscillation. From the laser-sheet data, the unsteady flow appeared as a massive boundary-layer separation followed by flow reattachment. Hot-wire data were taken in the wake to identify the presence of the flow oscillation and the dominant frequency. The oscillation was found in the flow from a Reynolds number of 0.3 to 1.3 x 10 exp 6. The Strouhal number based on airfoil projected height was nominally 0.02 and increased slightly with increasing Reynolds number and significantly with increasing airfoil angle of attack. Balow focused his research on the leading-edge separation bubble which was hypothesized to be the origin of the low-frequency oscillation. Initially, experimental measurements in the bubble at the onset of the low-frequency oscillation were attempted to study the characteristics of the bubble and explain possible relationships to the shear-layer-flapping phenomena. Unfortunately, the bubble proved to be extremely sensitive to the probe interference and it drastically reduced the size of the bubble. These detailed measurements were then abandoned by Balow. However, this led to a series of tests where the leading-edge bubble and trailing-edge separation were altered and the affect on the flow-oscillation studied. Balow found that by tripping the airfoil boundary-layer with "zigzag" tape ahead of bubble separation, the bubble was effectively eliminated mid the oscillation suppressed. Wake survey drag measurements showed a drastic reduction in airfoil drag when the bubble and oscillation were eliminated. Using the "zigzag" tape, the trailing-edge separation was moved downstream approximately 5 percent chord. This was found to reduce the amplitude of the oscillation, particularly in the onset stage at low angle of attack (around 14 degrees). Through detailed analysis of the wake behind the airfoil during the unsteady flow oscillation, Balow provided a better understanding of the wake flowfield. Broeren studied the oscillating flowfield in detail at Reynolds number equal 3 x 10 exp 5 and an angle of attack of 15 degrees using laser Doppler velocimetry (LDV). Two-dimensional LDV data were acquired at 687 grid points above the model upper surface while hot-wire data were taken simultaneously in the wake. Using the hot-wire signal, the LDV data were phase averaged into 24 bins to represent a single ensemble average of one oscillation cycle. The velocity data showed a flowfield oscillation that could be divided into three flow regimes. In the first regime, the flow over the airfoil was completely separated initially, the flowfield reattached from the leading edge and the reattachment point moved downstream with increasing time or phase. Broeren referred to this as the reattachment regime. The bubble development regime followed, where a leading-edge separation bubble formed at the leading edge and grew with increasing time. During the initial part of this regime the trailing-edge separation continued to move downstream. However, during the last 30 degrees of phase the trailing-edge separation moved rapidly forward and appeared to merge with the leading-edge bubble. During the third regime, the separation regime, the flow was segmented from the airfoil leading edge and did not reattach to the airfoil surface. The reverse flow was seen to grow in vertical extent up from the model surface as the phase increased. Next reattachment began again at the leading edge signaling the start of the reattachment regime, and so the cycle continued. From Broeren's work, the details of the unsteady flowfield over the airfoil were seen for the first time. From this research a great deal has been learned about the low-frequency flow oscillation which naturally occurs on the LRN-1007 airfoil near stall. The oscillation was seen to persist at higher Reynolds number, the dependence of the Strouhal number on angle of attack and Reynolds number were discovered, the critical role played by the laminar bubble was shown and the entire upper surface flowfield during a flow oscillation cycle was measured and analyzed. What still eludes understanding is the scaling of the flow oscillation and why certain airfoils, such as the LRN, have a very strong low-frequency mode and other airfoils exhibit no organized low-frequency oscillation at all.

Discussion of Priorities

NASA Technical Reports Server (NTRS)

2003-01-01

The Microgravity Science Division identifies four priority ratings for microgravity research and technology issues: 1) Critical; 2) Severely Limiting; 3) Enhancements; 4) Communication. Reduced gravity instabilities are critical, while severely limiting issues include phase separation, phase change, and flow through components. Enhancements are listed for passive phase separation and phase change. This viewgraph presentation also classifies microgravity issues as spaceflight, ground-based, or other for the time periods 2003-2008, 2009-2015, and beyond.

Novel platform for minimizing cell loss on separation process: Droplet-based magnetically activated cell separator

NASA Astrophysics Data System (ADS)

Kim, Youngho; Hong, Su; Lee, Sang Ho; Lee, Kangsun; Yun, Seok; Kang, Yuri; Paek, Kyeong-Kap; Ju, Byeong-Kwon; Kim, Byungkyu

2007-07-01

To reduce the problem of cell loss due to adhesion, one of the basic phenomena in microchannel, we proposed the droplet-based magnetically activated cell separator (DMACS). Based on the platform of the DMACS—which consists of permanent magnets, a coverslip with a circle-shaped boundary, and an injection tube—we could collect magnetically (CD45)-labeled (positive) cells with high purity and minimize cell loss due to adhesion. To compare separation efficiency between the MACS and the DMACS, the total number of cells before and after separation with both the separators was counted by flow cytometry. We could find that the number (3241/59940) of cells lost in the DMACS is much less than that (22360/59940) in the MACS while the efficiency of cell separation in the DMACS (96.07%) is almost the same as that in the MACS (96.72%). Practically, with fluorescent images, it was visually confirmed that the statistical data are reliable. From the viability test by using Hoechst 33 342, it was also demonstrated that there was no cell damage on a gas-liquid interface. Conclusively, DMACS will be a powerful tool to separate rare cells and applicable as a separator, key component of lab-on-a-chip.

Nanostructured pillars based on vertically aligned carbon nanotubes as the stationary phase in micro-CEC.

PubMed

Wu, Ren-Guei; Yang, Chung-Shi; Wang, Pen-Cheng; Tseng, Fan-Gang

2009-06-01

We present a micro-CEC chip carrying out a highly efficient separation of dsDNA fragments through vertically aligned multi-wall carbon nanotubes (MWCNTs) in a microchannel. The vertically aligned MWCNTs were grown directly in the microchannel to form straight nanopillar arrays as ordered and directional chromatographic supports. 1-Pyrenedodecanoic acid was employed for the surface modification of the MWCNTs' stationary phase to adsorb analytes by hydrophobic interactions. This device was used for separating dsDNA fragments of three different lengths (254, 360, and 572 bp), and fluorescence detection was employed to verify the electrokinetic transport in the MWCNT array. The micro-CEC separation of the three compounds was achieved in less than 300 s at a field strength of 66 V/cm due to superior laminar flow patterns and a lower flow resistance resulting from the vertically aligned MWCNTs being used as the stationary phase medium. In addition, a fivefold reduction of band broadening was obtained when the analyte was separated by the chromatographic MWCNT array channel instead of the CE channel. From all of the results, we suggest that an in situ grown and directional MWCNT array can potentially be useful for preparing more diversified forms of stationary phases for vertically efficient chip-based electrochromatography.

Membraneless laminar flow cell for electrocatalytic CO2 reduction with liquid product separation

NASA Astrophysics Data System (ADS)

Monroe, Morgan M.; Lobaccaro, Peter; Lum, Yanwei; Ager, Joel W.

2017-04-01

The production of liquid fuel products via electrochemical reduction of CO2 is a potential path to produce sustainable fuels. However, to be practical, a separation strategy is required to isolate the fuel-containing electrolyte produced at the cathode from the anode and also prevent the oxidation products (i.e. O2) from reaching the cathode. Ion-conducting membranes have been applied in CO2 reduction reactors to achieve this separation, but they represent an efficiency loss and can be permeable to some product species. An alternative membraneless approach is developed here to maintain product separation through the use of a laminar flow cell. Computational modelling shows that near-unity separation efficiencies are possible at current densities achievable now with metal cathodes via optimization of the spacing between the electrodes and the electrolyte flow rate. Laminar flow reactor prototypes were fabricated with a range of channel widths by 3D printing. CO2 reduction to formic acid on Sn electrodes was used as the liquid product forming reaction, and the separation efficiency for the dissolved product was evaluated with high performance liquid chromatography. Trends in product separation efficiency with channel width and flow rate were in qualitative agreement with the model, but the separation efficiency was lower, with a maximum value of 90% achieved.

Experimental parametric study of jet vortex generators for flow separation control

NASA Technical Reports Server (NTRS)

Selby, Gregory

1991-01-01

A parametric wind-tunnel study was performed with jet vortex generators to determine their effectiveness in controlling flow separation associated with low-speed turbulence flow over a two-dimensional rearward-facing ramp. Results indicate that flow-separation control can be accomplished, with the level of control achieved being a function of jet speed, jet orientation (with respect to the free-stream direction), and orifice pattern (double row of jets vs. single row). Compared to slot blowing, jet vortex generators can provide an equivalent level of flow control over a larger spanwise region (for constant jet flow area and speed). Dye flow visualization tests in a water tunnel indicated that the most effective jet vortex generator configurations produced streamwise co-rotating vortices.

Ducted fan inlet/exit and rotor tip flow improvements for vertical lift systems

NASA Astrophysics Data System (ADS)

Akturk, Ali

The current research utilized experimental and computational techniques in 5" and 22" diameter ducted fan test systems that have been custom designed and manufactured. Qualitative investigation of flow around the ducted fan was also performed using smoke flow visualizations. Quantitative measurements consisted of 2D and 3D velocity measurements using planar and Stereoscopic Particle Image Velocimetry (PIV and SPIV), high resolution total pressure measurements using Kiel total pressure probes and real time six-component force and torque measurements. The computational techniques used in this thesis included a recently developed radial equilibrium based rotor model(REBRM) and a three dimensional Reynolds-Averaged Navier Stokes (RANS) based CFD model. A radial equilibrium based rotor model (REBRM) developed by the author was effectively integrated into a three-dimensional RANS based computational system. The PIV measurements and computational flow predictions using (REBRM) near the fan inlet plane were in a good agreement at hover and forward flight conditions. The aerodynamic modifications resulting from the fan inlet flow distortions in forward flight regime were clearly captured in 2D PIV results. High resolution total pressure measurements at the downstream of the fan rotor showed that tip leakage, rotor hub separation, and passage flow related total pressure losses were dominant in hover condition. However, the losses were dramatically increased in forward flight because of inlet lip separation and distortion. A novel ducted fan inlet flow conditioning concept named "Double Ducted Fan" (DDF) was developed. The (DDF) concept has a potential to significantly improve the performance and controllability of VTOL UAVs and many other ducted fan based vertical lift systems. The new concept that will significantly reduce the inlet lip separation related performance penalties used a secondary stationary duct system to control "inlet lip separation" occurring especially at elevated forward flight velocities. The (DDF) is self-adjusting in a wide forward flight velocity range. DDFs corrective aerodynamic in influence becomes more pronounced with increasing flight velocity due to its inherent design properties. RANS simulations of the flow around rotor blades and duct geometry in the rotating frame of reference provided a comprehensive description of the tip leakage and passage flow in the flow environment of the two ducted fan research facilities developed throughout this thesis. The aerodynamic measurements and results of the RANS simulation showed good agreement especially near the tip region. A number of novel tip treatments based on custom designed pressure side extensions were introduced. Various tip leakage mitigation schemes were introduced by varying the chordwise location and the width of the extension in the circumferential direction. The current study showed that a proper selection of the pressure side bump location and width were the two critical parameters in influencing the success of the tip leakage mitigation approach. Significant gains in axial mean velocity component were observed when a proper pressure side tip extension was used. It is also observed that an effective tip leakage mitigation scheme significantly reduced the tangential velocity component near the tip of the axial fan blade. Reduced tip clearance related flow interactions were essential in improving the energy efficiency and range of ducted fan based vehicle. Full and inclined pressure side tip squealers were designed. Squealer tips were effective in changing the overall trajectory of the tip vortex to a higher path in radial direction. The interaction of rotor blades and tip vortex was effectively reduced and aerodynamic performance of the rotor blades was improved. The overall aerodynamic gain was a measurable reduction in leakage mass flow rate. This leakage reduction increased thrust significantly. Full and inclined pressure side tip squealers increased thrust obtained in hover condition by 9.1 % and 9.6 % respectively. A reduction or elimination of the momentum deficit in tip vortices is essential to reduce the adverse performance effects originating from the unsteady and highly turbulent tip leakage flows rotating against a stationary casing. The novel tip treatments developed throughout this thesis research are highly effective in reducing the adverse performance effects of ducted fan systems developed for VTOL vehicles. (Abstract shortened by UMI.)

Turbulence measurements in hypersonic shock-wave boundary-layer interaction flows

NASA Technical Reports Server (NTRS)

Mikulla, V.; Horstman, C. C.

1976-01-01

Turbulent intensity and Reynolds shear stress measurements are presented for two nonadiabatic hypersonic shock-wave boundary-layer interaction flows, one with and one without separation. These measurements were obtained using a new hot-wire probe specially designed for heated flows. Comparison of the separated and attached flows shows a significant increase above equilibrium values in the turbulent intensity and shear stress downstream of the interaction region for the attached case, while for the separated case, the turbulent fluxes remain close to equilibrium values. This effect results in substantial differences in turbulence lifetimes for the two flows. It is proposed that these differences are due to a coupling between the turbulent energy and separation bubble unsteadiness, a hypothesis supported by the statistical properties of the turbulent fluctuations.

Analysis of high-incidence separated flow past airfoils

NASA Technical Reports Server (NTRS)

Chia, K. N.; Osswald, G. A.; Chia, U.

1989-01-01

An unsteady Navier-Stokes (NS) analysis is developed and used to carefully examine high-incidence aerodynamic separated flows past airfoils. Clustered conformal C-grids are employed for the 12 percent thick symmetric Joukowski airfoil as well as for the NACA 0012 airfoil with a sharp trailing edge. The clustering is controlled by appropriate one-dimensional stretching transformations. An attempt is made to resolve many of the dominant scales of an unsteady flow with massive separation, while maintaining the transformation metrics to be smooth and continuous in the entire flow field. A fully implicit time-marching alternating-direction implicit-block Gaussian elimination (ADI-BGE) method is employed, in which no use is made of any explicit artificial dissipation. Detailed results are obtained for massively separated, unsteady flow past symmetric Joukowski and NACA 0012 airfoils.

Investigation of aerodynamic design issues with regions of separated flow

NASA Technical Reports Server (NTRS)

Gally, Tom

1993-01-01

Existing aerodynamic design methods have generally concentrated on the optimization of airfoil or wing shapes to produce a minimum drag while satisfying some basic constraints such as lift, pitching moment, or thickness. Since the minimization of drag almost always precludes the existence of separated flow, the evaluation and validation of these design methods for their robustness and accuracy when separated flow is present has not been aggressively pursued. However, two new applications for these design tools may be expected to include separated flow and the issues of aerodynamic design with this feature must be addressed. The first application of the aerodynamic design tools is the design of airfoils or wings to provide an optimal performance over a wide range of flight conditions (multipoint design). While the definition of 'optimal performance' in the multipoint setting is currently being hashed out, it is recognized that given a wide range of flight conditions, it will not be possible to ensure a minimum drag constraint at all conditions, and in fact some amount of separated flow (presumably small) may have to be allowed at the more demanding flight conditions. Thus a multipoint design method must be tolerant of the existence of separated flow and may include some controls upon its extent. The second application is in the design of wings with extended high speed buffet boundaries of their flight envelopes. Buffet occurs on a wing when regions of flow separation have grown to the extent that their time varying pressures induce possible destructive effects upon the wing structure or adversely effect either the aircraft controllability or passenger comfort. A conservative approach to the expansion of the buffet flight boundary is to simply expand the flight envelope of nonseparated flow under the assumption that buffet will also thus be alleviated. However, having the ability to design a wing with separated flow and thus to control the location, extent and severity of the separated flow regions may allow aircraft manufacturers to gain an advantage in the early design stages of an aircraft, when configuration changes are relatively inexpensive to make. The goal of the summer research at NASA Langley Research Center (LaRC) was twofold: first, to investigate a particular airfoil design problem observed under conditions of strong shock induced flow separation on the upper surface of an airfoil at transonic conditions; and second, to suggest and investigate design methodologies for the prediction (or detection) and control of flow separation. The context of both investigations was to use an existing two dimensional Navier-Stokes flow solver and the constrained direct/iterative surface curvature (CDISC) design algorithm developed at LaRC. As a lead in to the primary task, it was necessary to gain a familiarity with both the design method and the computational analysis and to perform the FORTRAN coding needed to couple them together.

An investigation of paper based microfluidic devices for size based separation and extraction applications.

PubMed

Zhong, Z W; Wu, R G; Wang, Z P; Tan, H L

2015-09-01

Conventional microfluidic devices are typically complex and expensive. The devices require the use of pneumatic control systems or highly precise pumps to control the flow in the devices. This work investigates an alternative method using paper based microfluidic devices to replace conventional microfluidic devices. Size based separation and extraction experiments conducted were able to separate free dye from a mixed protein and dye solution. Experimental results showed that pure fluorescein isothiocyanate could be separated from a solution of mixed fluorescein isothiocyanate and fluorescein isothiocyanate labeled bovine serum albumin. The analysis readings obtained from a spectrophotometer clearly show that the extracted tartrazine sample did not contain any amount of Blue-BSA, because its absorbance value was 0.000 measured at a wavelength of 590nm, which correlated to Blue-BSA. These demonstrate that paper based microfluidic devices, which are inexpensive and easy to implement, can potentially replace their conventional counterparts by the use of simple geometry designs and the capillary action. These findings will potentially help in future developments of paper based microfluidic devices. Copyright © 2015 Elsevier B.V. All rights reserved.

An analysis of the crossover between local and massive separation on airfoils

NASA Technical Reports Server (NTRS)

Barnett, M.; Carter, J. E.

1987-01-01

Massive separation on airfoils operating at high Reynolds number is an important problem to the aerodynamicist, since its onset generally determines the limiting performance of an airfoil, and it can lead to serious problems related to aircraft control as well as turbomachinery operation. The phenomenon of crossover between local separation and massive separation on realistic airfoil geometries induced by airfoil thickness is investigated for low speed (incompressible) flow. The problem is studied both for the asymptotic limit of infinite Reynolds number using triple-deck theory, and for finite Reynolds number using interacting boundary-layer theory. Numerical results are presented which follow the evolution of the flow as it develops from a mildly separated state to one dominated by the massively separated flow structure as the thickness of the airfoil geometry is systematically increased. The effect of turbulence upon the evolution of the flow is considered, and the impact is significant, with the principal effect being the suppression of the onset of separation. Finally, the effect of surface suction and injection for boundary-layer control is considered. The approach which was developed provides a valuable tool for the analysis of boundary-layer separation up to and beyond stall. Another important conclusion is that interacting boundary-layer theory provides an efficient tool for the analysis of the effect of turbulence and boundary-layer control upon separated vicsous flow.

Numerical study of the SSME nozzle flow fields during transient operations: A comparison of the animated results with test

NASA Technical Reports Server (NTRS)

Wang, Ten-See; Dumas, Catherine

1993-01-01

A computational fluid dynamics (CFD) model has been applied to study the transient flow phenomena of the nozzle and exhaust plume of the Space Shuttle Main Engine (SSME), fired at sea level. The CFD model is a time accurate, pressure based, reactive flow solver. A six-species hydrogen/oxygen equilibrium chemistry is used to describe the chemical-thermodynamics. An adaptive upwinding scheme is employed for the spatial discretization, and a predictor, multiple corrector method is used for the temporal solution. Both engine start-up and shut-down processes were simulated. The elapse time is approximately five seconds for both cases. The computed results were animated and compared with the test. The images for the animation were created with PLOT3D and FAST and then animated with ABEKAS. The hysteresis effects, and the issues of free-shock separation, restricted-shock separation and the end-effects were addressed.

Effect of Stagger on the Vibroacoustic Loads from Clustered Rockets

NASA Technical Reports Server (NTRS)

Rojo, Raymundo; Tinney, Charles E.; Ruf, Joseph H.

2016-01-01

The effect of stagger startup on the vibro-acoustic loads that form during the end- effects-regime of clustered rockets is studied using both full-scale (hot-gas) and laboratory scale (cold gas) data. Both configurations comprise three nozzles with thrust optimized parabolic contours that undergo free shock separated flow and restricted shock separated flow as well as an end-effects regime prior to flowing full. Acoustic pressure waveforms recorded at the base of the nozzle clusters are analyzed using various statistical metrics as well as time-frequency analysis. The findings reveal a significant reduction in end- effects-regime loads when engine ignition is staggered. However, regardless of stagger, both the skewness and kurtosis of the acoustic pressure time derivative elevate to the same levels during the end-effects-regime event thereby demonstrating the intermittence and impulsiveness of the acoustic waveforms that form during engine startup.

Dielectrophoretic systems without embedded electrodes

DOEpatents

Cummings, Eric B [Livermore, CA; Singh, Anup K [San Francisco, CA

2006-03-21

Method and apparatus for dielectrophoretic separation of particles in a fluid based using array of insulating structures arranged in a fluid flow channel. By utilizing an array of insulating structures, a spatially inhomogeneous electric field is created without the use of the embedded electrodes conventionally employed for dielectrophoretic separations. Moreover, by using these insulating structures a steady applied electric field has been shown to provide for dielectrophoresis in contrast to the conventional use of an alternating electric field. In a uniform array of posts, dielectrophoretic effects have been produced flows having significant pressure-driven and electrokinetic transport. Above a threshold applied electric field, filaments of concentrated and rarefied particles appear in the flow as a result of dielectrophoresis. Above a higher threshold applied voltage, dielectrophoresis produces zones of highly concentrated and immobilized particles. These patterns are strongly influenced by the angle of the array of insulating structures with respect to the mean applied electric field and the shape of the insulating structures.

A note on flow reversal in a wavy channel filled with anisotropic porous material

NASA Astrophysics Data System (ADS)

Karmakar, Timir; Raja Sekhar, G. P.

2017-07-01

Viscous flow through a symmetric wavy channel filled with anisotropic porous material is investigated analytically. Flow inside the porous bed is assumed to be governed by the anisotropic Brinkman equation. It is assumed that the ratio of the channel width to the wavelength is small (i.e. δ2≪1). The problem is solved up to O(δ2) assuming that δ2λ2≪1, where λ is the anisotropic ratio. The key purpose of this paper is to study the effect of anisotropic permeability on flow near the crests of the wavy channel which causes flow reversal. We present a detailed analysis of the flow reversal at the crests. The ratio of the permeabilities (anisotropic ratio) is responsible for the flow separation near the crests of the wall where viscous forces are effective. For a flow configuration (say, low amplitude parameter) in which there is no separation if the porous media is isotropic, introducing anisotropy causes flow separation. On the other hand, interestingly, flow separation occurs even in the case of isotropic porous medium if the amplitude parameter a is large.

Separation control on the wing by jet actuators

NASA Astrophysics Data System (ADS)

Karyakin, O. M.; Nalivaiko, A. G.; Ustinov, M. V.; Flaxman, Ja. Sh.

2018-05-01

Use of jet actuators to eliminate flow separation is experimentally investigated on a straight wing with a NACA 0012 airfoil. It is shown that under the influence of synthetic jets the size of separation zone greatly reduces and the flow separation point displaces downstream. In addition, lift coefficient increases by more than 10%.

Using the developed cross-flow filtration chip for collecting blood plasma under high flow rate condition and applying the immunoglobulin E detection

NASA Astrophysics Data System (ADS)

Yeh, Chia-Hsien; Hung, Chia-Wei; Wu, Chun-Han; Lin, Yu-Cheng

2014-09-01

This paper presents a cross-flow filtration chip for separating blood cells (white blood cells, red blood cells, and platelets) and obtaining blood plasma from human blood. Our strategy is to flow the sample solution in parallel to the membrane, which can generate a parallel shear stress to remove the clogging microparticles on the membrane, so the pure sample solution is obtained in the reservoir. The cross-flow filtration chip includes a cross-flow layer, a Ni-Pd alloy micro-porous membrane, and a reservoir layer. The three layers are packaged in a polymethylmethacrylate (PMMA) frame to create the cross-flow filtration chip. Various dilutions of the blood sample (original, 2 × , 3 × , 5 × , and 10×), pore sizes with different diameters (1 µm, 2 µm, 4 µm, 7 µm, and 10 µm), and different flow rates (1 mL/min, 3 mL/min, 5 mL/min, 7 mL/min, and 10 mL/min) are tested to determine their effects on filtration percentage. The best filtration percentage is 96.2% when the dilution of the blood sample is 10 × , the diameter of pore size of a Ni-Pd alloy micro-porous membrane is 2 µm, and the flow rate is 10 mL/min. Finally, for the clinical tests of the immunoglobulin E (IgE) concentration, the cross-flow filtration chip is used to filter the blood of the allergy patients to obtain the blood plasma. This filtered blood plasma is compared with that obtained using the conventional centrifugation based on the enzyme-linked immunosorbent assay. The results reveal that these two blood separation methods have similar detection trends. The proposed filtration chip has the advantages of low cost, short filtration time, and easy operation and thus can be applied to the separation of microparticles, cells, bacteria, and blood.

High efficiency virtual impactor

DOEpatents

Loo, Billy W.

1981-01-01

Environmental monitoring of atmospheric air is facilitated by a single stage virtual impactor (11) for separating an inlet flow (Q.sub.O) having particulate contaminants into a coarse particle flow (Q.sub.1) and a fine particle flow (Q.sub.2) to enable collection of such particles on different filters (19a, 19b) for separate analysis. An inlet particle acceleration nozzle (28) and coarse particle collection probe member (37) having a virtual impaction opening (41) are aligned along a single axis (13) and spaced apart to define a flow separation region (14) at which the fine particle flow (Q.sub.2) is drawn radially outward into a chamber (21) while the coarse particle flow (Q.sub.1) enters the virtual impaction opening (41). Symmetrical outlet means (47) for the chamber (21) provide flow symmetry at the separation region (14) to assure precise separation of particles about a cutpoint size and to minimize losses by wall impaction and gravitational settling. Impulse defocusing means (42) in the probe member (37) provides uniform coarse particle deposition on the filter (19a) to aid analysis. Particle losses of less than 1% for particles in the 0 to 20 micron range may be realized.

Corner separation and the onset of stall in an axial compressor

NASA Astrophysics Data System (ADS)

Thiam, Aicha; Whittlesey, Robert; Wark, Candace; Williams, David

2007-11-01

Axial compressor performance is limited by the onset of stall between the diffusing passageways of the rotors and stators. The flow physics responsible for the stall depends on the blade geometry of the machine, and in this experiment stall develops from a blade-hub corner separation. The 1.5 stage axial compressor consists of inlet guide vanes, a rotor and stator section. Separate motors drive the downstream fan and rotor, which makes it possible to change the compressor pressure ratio and flow coefficient by changing either the wheel speed or the bulk flow rate through the machine. Detailed maps of the flow behind the stators and in front of the rotors were obtained using a Kulite stagnation pressure probe. Mean pressure measurements show the growth of the corner flow separation and divergence of the ``through flow'' toward the outer casing. Spectra show a sensitivity of the separated region to small amplitude external disturbances, in this case originating from the downstream fan. The onset of rotating stall appears as the first subharmonic of the rotor frequency, 0.5 fr, then shifts to a slightly lower frequency 0.45 fr as the flow coefficient is decreased.

Thermally assisted acoustophoresis as a new stiffness-based separation method

NASA Astrophysics Data System (ADS)

Dolatmoradi, Ata; El-Zahab, Bilal

2017-02-01

The use of acoustophoretic separation devices provides a feasible means in biomedical diagnostics for label-free separation of diseased cells. Separation via acoustophoresis, however, has been restricted mainly to size contrast. Thermally-assisted acoustophoresis, as a newly-developed approach, integrates acoustic and thermal actuators on the same platform, enabling a stiffness-based separation when adjusted properly. Using this method, we have demonstrated the possibility of separating cell-mimicking liposomes based on their membrane stiffness. In a temperature-tuned microchannel with an overlaid ultrasonic standing wave, the acoustic contrast factor of a liposome is mainly determined according to its compressibility compared to that of medium. The sign of this factor was observed to flip to a negative value at a specific temperature, unique to the composition of the liposome. This sign switch was hypothesized to be due to the thermotropic phase transitions in the liposome's membrane upon which an apparent effect on the compressibility is experienced by the liposome. By choosing the midpoint of the existing temperature window for two different compositions, within which liposomes were mechanically distinct enough to become differentiable in the acoustic radiation field, we examined the separation efficiency under different flow rate conditions.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R.; Torczynski, John R.; Brady, Patrick V.; Gallis, Michail; Brooks, Carlton F.

2014-06-17

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R; Torczynski, John R; Brady, Patrick V; Gallis, Michail; Brooks, Carlton F

2013-09-17

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R; Torczynski, John R; Brady, Patrick V; Gallis, Michail; Brooks, Carlton F

2013-11-19

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Vortex developments over steady and accelerated airfoils incorporating a trailing edge jet

NASA Technical Reports Server (NTRS)

Finaish, F.; Okong'o, N.; Frigerio, J.

1993-01-01

Computational and experimental studies are conducted to investigate the influence of a trailing edge jet on flow separation and subsequent vortex formation over steady and accelerated airfoils at high angles of attack. A computer code, employing the stream function-vorticity approach, is developed and utilized to conduct numerical experiments on the flow problem. To verify and economize such efforts, an experimental system is developed and incorporated into a subsonic wind tunnel where streamline and vortex flow visualization experiments are conducted. The study demonstrates the role of the trailing edge jet in controlling flow separation and subsequent vortex development for steady and accelerating flow at angles past the static stall angle of attack. The results suggest that the concept of the trailing edge jet may be utilized to control the characteristics of unsteady separated flows over lifting surfaces. This control possibility seems to be quite effective and could have a significant role in controlling unsteady separated flows.

[Present situation and development trends of asymmetrical flow field-flow fractionation].

PubMed

Liang, Qihui; Wu, Di; Qiu, Bailing; Han, Nanyin

2017-09-08

Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field). Asymmetrical flow field-flow fractionation (AF4) is a vital subvariant of FFF, which applying a vertical flow field as the second dimension force field. The separation in AF4 opening channel is carried out by any composition carrier fluid, universally and effectively used in separation of bioparticles and biopolymers due to the non-invasivity feature. Herein, bio-analytes are held in bio-friendly environment and easily sterilized without using degrading carrier fluid which is conducive to maintain natural conformation. In this review, FFF and AF4 principles are briefly described, and some classical and emerging applications and developments in the bioanalytical fields are concisely introduced and tabled. Also, special focus is given to the hyphenation of AF4 with highly specific, sensitive detection technologies.

Flow control in axial fan inlet guide vanes by synthetic jets

NASA Astrophysics Data System (ADS)

Cyrus, V.; Trávníček, Z.; Wurst, P.; Kordík, J.

2013-04-01

Tested high pressure axial flow fan with hub/tip ratio of 0.70 and external diameter of 600 mm consisted of inlet guide vanes (IGV), rotor and stator blade rows. Fan peripheral velocity was 47 m/s. Air volume flow rate was changed by turning of rear part of the inlet guide vanes. At turning of 20 deg the flow was separated on the IGV profiles. The synthetic jets were introduced through radial holes in machine casing in the location before flow separation origin. Synthetic jet actuator was designed with the use of a speaker by UT AVCR. Its membrane had diameter of 63 mm. Excitation frequency was chosen in the range of 500 Hz - 700 Hz. Synthetic jets favourably influenced separated flow on the vane profiles in the distance of (5 - 12) mm from the casing surface. The reduction of flow separation area caused in the region near the casing the decrease of the profile loss coefficient approximately by 20%.

Assessment of the viability of skin grafts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wahner, H.W.

1988-07-01

A number of tests are available to monitor the blood flow in free and distant pedicle skin grafts. The information from these tests aids in the development of measures to enhance vascularization and is occasionally needed to make clinical decisions in patients with distant pedicle grafts. Measurements of the disappearance of an intradermally injected small amount of /sup 133/Xe allows determination of a clearance rate and blood flow before and after clamping the original blood supply through the base. With /sup 99m/Tc, which is generally more readily available, a flow index and block index can be determined. Clinically both proceduresmore » give equally good results in determining a safe time for pedicle base separation. The fluorescein test allows assessment of regional blood flow distribution within the pedicle.« less

Debris-flow deposits and watershed erosion rates near southern Death Valley, CA, United States

USGS Publications Warehouse

Schmidt, K.M.; Menges, C.M.; ,

2003-01-01

Debris flows from the steep, granitic hillslopes of the Kingston Range, CA are commensurate in age with nearby fluvial deposits. Quaternary chronostratigraphic differentiation of debris-flow deposits is based upon time-dependent characteristics such as relative boulder strength, derived from Schmidt Hammer measurements, degree of surface desert varnish, pedogenesis, and vertical separation. Rock strength is highest for Holocene-aged boulders and decreases for Pleistocene-aged boulders weathering to grus. Volumes of age-stratified debris-flow deposits, constrained by deposit thickness above bedrock, GPS surveys, and geologic mapping, are greatest for Pleistocene deposits. Shallow landslide susceptibility, derived from a topographically based GIS model, in conjunction with deposit volumes produces watershed-scale erosion rates of ???2-47 mm ka-1, with time-averaged Holocene rates exceeding Pleistocene rates. ?? 2003 Millpress.

Microfluidic co-flow of Newtonian and viscoelastic fluids for high-resolution separation of microparticles.

PubMed

Tian, Fei; Zhang, Wei; Cai, Lili; Li, Shanshan; Hu, Guoqing; Cong, Yulong; Liu, Chao; Li, Tiejun; Sun, Jiashu

2017-09-12

The microfluidic passive control of microparticles largely relies on the hydrodynamic effects of the carrier media such as Newtonian fluids and viscoelastic fluids. Yet the viscoelastic/Newtonian interfacial effect has been scarcely investigated, especially for high-resolution particle separation. Here we report a microfluidic co-flow of Newtonian (water or PBS) and viscoelastic fluids (PEO) for the size-dependent separation of microparticles. The co-flow condition generates a stable viscoelastic/Newtonian interface, giving rise to the wall-directed elastic lift forces that compete with the center-directed lift forces, and efficiently hinders the migration of microparticles from the Newtonian to the viscoelastic fluid in a size-dependent manner. An almost complete separation of a binary mixture of 1 μm and 2 μm polystyrene particles is achieved by the co-flow of water and a very dilute PEO solution (100 ppm), whereas the sole use of water or PEO could not lead to an efficient separation. This co-flow microfluidic system is also applied for the separation of Staphylococcus aureus (1 μm) from platelets (2-3 μm) with >90% efficiencies and purities.

Computation of turbulent flows over backward and forward-facing steps using a near-wall Reynolds stress model

NASA Technical Reports Server (NTRS)

Ko, Sung HO

1993-01-01

Separation and reattachment of turbulent shear layers is observed in many important engineering applications, yet it is poorly understood. This has motivated many studies on understanding and predicting the processes of separation and reattachment of turbulent shear layers. Both of the situations in which separation is induced by adverse pressure gradient, or by discontinuities of geometry, have attracted attention of turbulence model developers. Formulation of turbulence closure models to describe the essential features of separated turbulent flows accurately is still a formidable task. Computations of separated flows associated with sharp-edged bluff bodies are described. For the past two decades, the backward-facing step flow, the simplest separated flow, has been a popular test case for turbulence models. Detailed studies on the performance of many turbulence models, including two equation turbulence models and Reynolds stress models, for flows over steps can be found in the papers by Thangam & Speziale and Lasher & Taulbee). These studies indicate that almost all the existing turbulence models fail to accurately predict many important features of back step flow such as reattachment length, recovery rate of the redeveloping boundary layers downstream of the reattachment point, streamlines near the reattachment point, and the skin friction coefficient. The main objectives are to calculate flows over backward and forward-facing steps using the NRSM and to make use of the newest DNS data for detailed comparison. This will give insights for possible improvements of the turbulence model.

Closed-loop Separation Control Using Oscillatory Flow Excitation

NASA Technical Reports Server (NTRS)

Allan, Brian G.; Juang, Jer-Nan; Raney, David L.; Seifert, Avi; Pack, latunia G.; Brown, Donald E.

2000-01-01

Design and implementation of a digital feedback controller for a flow control experiment was performed. The experiment was conducted in a cryogenic pressurized wind tunnel on a generic separated configuration at a chord Reynolds number of 16 million and a Mach number of 0.25. The model simulates the upper surface of a 20% thick airfoil at zero angle-of-attack. A moderate favorable pressure gradient, up to 55% of the chord, is followed by a severe adverse pressure gradient which is relaxed towards the trailing edge. The turbulent separation bubble, behind the adverse pressure gradient, is then reduced by introducing oscillatory flow excitation just upstream of the point of flow separation. The degree of reduction in the separation region can be controlled by the amplitude of the oscillatory excitation. A feedback controller was designed to track a given trajectory for the desired degree of flow reattachment and to improve the transient behavior of the flow system. Closed-loop experiments demonstrated that the feedback controller was able to track step input commands and improve the transient behavior of the open-loop response.

High-throughput separation of cells by dielectrophoresis enhanced with 3D gradient AC electric field.

PubMed

Tada, Shigeru; Hayashi, Masako; Eguchi, Masanori; Tsukamoto, Akira

2017-11-01

We propose a novel, high-performance dielectrophoretic (DEP) cell-separation flow chamber with a parallel-plate channel geometry. The flow chamber, consisting of a planar electrode on the top and an interdigitated-pair electrode array at the bottom, was developed to facilitate the separation of cells by creating a nonuniform AC electric field throughout the volume of the flow chamber. The operation and performance of the device were evaluated using live and dead human epithermal breast (MCF10A) cells. The separation dynamics of the cell suspension in the flow chamber was also investigated by numerically simulating the trajectories of individual cells. A theoretical model to describe the dynamic cell behavior under the action of DEP, including dipole-dipole interparticle, viscous, and gravitational forces, was developed. The results demonstrated that the live cells traveling through the flow chamber congregated into sites where the electric field gradient was minimal, in the middle of the flow stream slightly above the centerlines of the grounded electrodes at the bottom. Meanwhile, the dead cells were trapped on the edges of the high-voltage electrodes at the bottom. Cells were thus successfully separated with a remarkably high separation ratio (∼98%) at the appropriately tuned field frequency and applied voltage. The numerically predicted behavior and spatial distribution of the cells during separation also showed good agreement with those observed experimentally.

The lagRST Model: A Turbulence Model for Non-Equilibrium Flows

NASA Technical Reports Server (NTRS)

Lillard, Randolph P.; Oliver, A. Brandon; Olsen, Michael E.; Blaisdell, Gregory A.; Lyrintzis, Anastasios S.

2011-01-01

This study presents a new class of turbulence model designed for wall bounded, high Reynolds number flows with separation. The model addresses deficiencies seen in the modeling of nonequilibrium turbulent flows. These flows generally have variable adverse pressure gradients which cause the turbulent quantities to react at a finite rate to changes in the mean flow quantities. This "lag" in the response of the turbulent quantities can t be modeled by most standard turbulence models, which are designed to model equilibrium turbulent boundary layers. The model presented uses a standard 2-equation model as the baseline for turbulent equilibrium calculations, but adds transport equations to account directly for non-equilibrium effects in the Reynolds Stress Tensor (RST) that are seen in large pressure gradients involving shock waves and separation. Comparisons are made to several standard turbulence modeling validation cases, including an incompressible boundary layer (both neutral and adverse pressure gradients), an incompressible mixing layer and a transonic bump flow. In addition, a hypersonic Shock Wave Turbulent Boundary Layer Interaction with separation is assessed along with a transonic capsule flow. Results show a substantial improvement over the baseline models for transonic separated flows. The results are mixed for the SWTBLI flows assessed. Separation predictions are not as good as the baseline models, but the over prediction of the peak heat flux downstream of the reattachment shock that plagues many models is reduced.

System and method for treatment of a medium

DOE Office of Scientific and Technical Information (OSTI.GOV)

Singh, Surinder Prabhjot; Acharya, Harish Radhakrishna; Perry, Robert James

2017-05-23

A system and method for treatment of a medium is disclosed. The system includes a plurality of separator zones and a plurality of heat transfer zones. Each of the separator zone and the heat transfer zone among the plurality of separator zones and heat transfer zones respectively, are disposed alternatively in a flow duct. Further, each separator zone includes an injector device for injecting a sorbent into the corresponding separator zone. Within the corresponding separator zone, the injected sorbent is reacted with a gaseous medium flowing in the flow duct, so as to generate a reacted gaseous medium and amore » reacted sorbent. Further, each heat transfer zone exchanges heat between the reacted gaseous medium fed from the corresponding separator zone and a heat transfer medium.« less

Development of the Two Phase Flow Separator Experiment for a Reduced Gravity Aircraft Flight

NASA Technical Reports Server (NTRS)

Golliher, Eric; Gotti, Daniel; Owens, Jay; Gilkey, Kelly; Pham, Nang; Stehno, Philip

2016-01-01

The recent hardware development and testing of a reduced gravity aircraft flight experiment has provided valuable insights for the future design of the Two Phase Flow Separator Experiment (TPFSE). The TPFSE is scheduled to fly within the Fluids Integration Rack (FIR) aboard the International Space Station (ISS) in 2020. The TPFSE studies the operational limits of gas and liquid separation of passive cyclonic separators. A passive cyclonic separator utilizes only the inertia of the incoming flow to accomplish the liquid-gas separation. Efficient phase separation is critical for environmental control and life support systems, such as recovery of clean water from bioreactors, for long duration human spaceflight missions. The final low gravity aircraft flight took place in December 2015 aboard NASA's C9 airplane.

On an interface of the online system for a stochastic analysis of the varied information flows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gorshenin, Andrey K.; MIREA, MGUPI; Kuzmin, Victor Yu.

The article describes a possible approach to the construction of an interface of an online asynchronous system that allows researchers to analyse varied information flows. The implemented stochastic methods are based on the mixture models and the method of moving separation of mixtures. The general ideas of the system functionality are demonstrated on an example for some moments of a finite normal mixture.

Computational Models of Laryngeal Aerodynamics: Potentials and Numerical Costs.

PubMed

Sadeghi, Hossein; Kniesburges, Stefan; Kaltenbacher, Manfred; Schützenberger, Anne; Döllinger, Michael

2018-02-07

Human phonation is based on the interaction between tracheal airflow and laryngeal dynamics. This fluid-structure interaction is based on the energy exchange between airflow and vocal folds. Major challenges in analyzing the phonatory process in-vivo are the small dimensions and the poor accessibility of the region of interest. For improved analysis of the phonatory process, numerical simulations of the airflow and the vocal fold dynamics have been suggested. Even though most of the models reproduced the phonatory process fairly well, development of comprehensive larynx models is still a subject of research. In the context of clinical application, physiological accuracy and computational model efficiency are of great interest. In this study, a simple numerical larynx model is introduced that incorporates the laryngeal fluid flow. It is based on a synthetic experimental model with silicone vocal folds. The degree of realism was successively increased in separate computational models and each model was simulated for 10 oscillation cycles. Results show that relevant features of the laryngeal flow field, such as glottal jet deflection, develop even when applying rather simple static models with oscillating flow rates. Including further phonatory components such as vocal fold motion, mucosal wave propagation, and ventricular folds, the simulations show phonatory key features like intraglottal flow separation and increased flow rate in presence of ventricular folds. The simulation time on 100 CPU cores ranged between 25 and 290 hours, currently restricting clinical application of these models. Nevertheless, results show high potential of numerical simulations for better understanding of phonatory process. Copyright © 2018 The Voice Foundation. Published by Elsevier Inc. All rights reserved.

Development of an Efficient Meso- scale Multi-phase Flow Solver in Nuclear Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Taehun

2015-10-20

The proposed research aims at formulating a predictive high-order Lattice Boltzmann Equation for multi-phase flows relevant to nuclear energy related application - namely, saturated and sub-cooled boiling in reactors, and liquid- liquid mixing and extraction for fuel cycle separation. An efficient flow solver will be developed based on the Finite Element based Lattice Boltzmann Method (FE- LBM), accounting for phase-change heat transfer and capable of treating multiple phases over length scales from the submicron to the meter. A thermal LBM will be developed in order to handle adjustable Prandtl number, arbitrary specific heat ratio, a wide range of temperature variations,more » better numerical stability during liquid-vapor phase change, and full thermo-hydrodynamic consistency. Two-phase FE-LBM will be extended to liquid–liquid–gas multi-phase flows for application to high-fidelity simulations building up from the meso-scale up to the equipment sub-component scale. While several relevant applications exist, the initial applications for demonstration of the efficient methods to be developed as part of this project include numerical investigations of Critical Heat Flux (CHF) phenomena in nuclear reactor fuel bundles, and liquid-liquid mixing and interfacial area generation for liquid-liquid separations. In addition, targeted experiments will be conducted for validation of this advanced multi-phase model.« less

Determining Aqueous Fullerene Particle Size Distributions by Asymmetric Flow Field-Flow Fractionation (AF4) without Surfactants

EPA Science Inventory

To determine the behavior of nanoparticles in environmental systems, methods must be developed to measure nanoparticle size. Asymmetric Flow Field Flow Fractionation (AF4) is an aqueous compatible size separation technique which is able to separate particles from 1 nm to 10 µm in...

Separation and quantification of monoclonal-antibody aggregates by hollow-fiber-flow field-flow fractionation.

PubMed

Fukuda, Jun; Iwura, Takafumi; Yanagihara, Shigehiro; Kano, Kenji

2014-10-01

Hollow-fiber-flow field-flow fractionation (HF5) separates protein molecules on the basis of the difference in the diffusion coefficient, and can evaluate the aggregation ratio of proteins. However, HF5 is still a minor technique because information on the separation conditions is limited. We examined in detail the effect of different settings, including the main-flow rate, the cross-flow rate, the focus point, the injection amount, and the ionic strength of the mobile phase, on fractographic characteristics. On the basis of the results, we proposed optimized conditions of the HF5 method for quantification of monoclonal antibody in sample solutions. The HF5 method was qualified regarding the precision, accuracy, linearity of the main peak, and quantitation limit. In addition, the HF5 method was applied to non-heated Mab A and heat-induced-antibody-aggregate-containing samples to evaluate the aggregation ratio and the distribution extent. The separation performance was comparable with or better than that of conventional methods including analytical ultracentrifugation-sedimentation velocity and asymmetric-flow field-flow fractionation.

High-Performance Oligomeric Catholytes for Effective Macromolecular Separation in Nonaqueous Redox Flow Batteries

PubMed Central

2018-01-01

Nonaqueous redox flow batteries (NRFBs) represent an attractive technology for energy storage from intermittent renewable sources. In these batteries, electrical energy is stored in and extracted from electrolyte solutions of redox-active molecules (termed catholytes and anolytes) that are passed through an electrochemical flow cell. To avoid battery self-discharge, the anolyte and catholyte solutions must be separated by a membrane in the flow cell. This membrane prevents crossover of the redox active molecules, while simultaneously allowing facile transport of charge-balancing ions. A key unmet challenge for the field is the design of redox-active molecule/membrane pairs that enable effective electrolyte separation while maintaining optimal battery properties. Herein, we demonstrate the development of oligomeric catholytes based on tris(dialkylamino)cyclopropenium (CP) salts that are specifically tailored for pairing with size-exclusion membranes composed of polymers of intrinsic microporosity (PIMs). Systematic studies were conducted to evaluate the impact of oligomer size/structure on properties that are crucial for flow battery performance, including cycling stability, charge capacity, solubility, electron transfer kinetics, and crossover rates. These studies have led to the identification of a CP-derived tetramer in which these properties are all comparable, or significantly improved, relative to the monomeric counterpart. Finally, a proof-of-concept flow battery is demonstrated by pairing this tetrameric catholyte with a PIM membrane. After 6 days of cycling, no crossover is detected, demonstrating the promise of this approach. These studies provide a template for the future design of other redox-active oligomers for this application. PMID:29532018

High-Performance Oligomeric Catholytes for Effective Macromolecular Separation in Nonaqueous Redox Flow Batteries

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hendriks, Koen H.; Robinson, Sophia G.; Braten, Miles N.

Nonaqueous redox flow batteries (NRFBs) represent an attractive technology for energy storage from intermittent renewable sources. In these batteries, electrical energy is stored in and extracted from electrolyte solutions of redox-active molecules (termed catholytes and anolytes) that are passed through an electrochemical flow cell. To avoid battery self-discharge, the anolyte and catholyte solutions must be separated by a membrane in the flow cell. This membrane prevents crossover of the redox active molecules, while simultaneously allowing facile transport of charge-balancing ions. A key unmet challenge for the field is the design of redox-active molecule/membrane pairs that enable effective electrolyte separation whilemore » maintaining optimal battery properties. Herein, we demonstrate the development of oligomeric catholytes based on tris(dialkylamino)cyclopropenium (CP) salts that are specifically tailored for pairing with size-exclusion membranes composed of polymers of intrinsic microporosity (PIMs). Systematic studies were conducted to evaluate the impact of oligomer size/structure on properties that are crucial for flow battery performance, including cycling stability, charge capacity, solubility, electron transfer kinetics, and crossover rates. These studies have led to the identification of a CP-derived tetramer in which these properties are all comparable, or significantly improved, relative to the monomeric counterpart. Finally, a proof-of-concept flow battery is demonstrated by pairing this tetrameric catholyte with a PIM membrane. After 6 days of cycling, no crossover is detected, demonstrating the promise of this approach. Finally, these studies provide a template for the future design of other redox-active oligomers for this application.« less

High-Performance Oligomeric Catholytes for Effective Macromolecular Separation in Nonaqueous Redox Flow Batteries.

PubMed

Hendriks, Koen H; Robinson, Sophia G; Braten, Miles N; Sevov, Christo S; Helms, Brett A; Sigman, Matthew S; Minteer, Shelley D; Sanford, Melanie S

2018-02-28

Nonaqueous redox flow batteries (NRFBs) represent an attractive technology for energy storage from intermittent renewable sources. In these batteries, electrical energy is stored in and extracted from electrolyte solutions of redox-active molecules (termed catholytes and anolytes) that are passed through an electrochemical flow cell. To avoid battery self-discharge, the anolyte and catholyte solutions must be separated by a membrane in the flow cell. This membrane prevents crossover of the redox active molecules, while simultaneously allowing facile transport of charge-balancing ions. A key unmet challenge for the field is the design of redox-active molecule/membrane pairs that enable effective electrolyte separation while maintaining optimal battery properties. Herein, we demonstrate the development of oligomeric catholytes based on tris(dialkylamino)cyclopropenium (CP) salts that are specifically tailored for pairing with size-exclusion membranes composed of polymers of intrinsic microporosity (PIMs). Systematic studies were conducted to evaluate the impact of oligomer size/structure on properties that are crucial for flow battery performance, including cycling stability, charge capacity, solubility, electron transfer kinetics, and crossover rates. These studies have led to the identification of a CP-derived tetramer in which these properties are all comparable, or significantly improved, relative to the monomeric counterpart. Finally, a proof-of-concept flow battery is demonstrated by pairing this tetrameric catholyte with a PIM membrane. After 6 days of cycling, no crossover is detected, demonstrating the promise of this approach. These studies provide a template for the future design of other redox-active oligomers for this application.

High-Performance Oligomeric Catholytes for Effective Macromolecular Separation in Nonaqueous Redox Flow Batteries

DOE PAGES

Hendriks, Koen H.; Robinson, Sophia G.; Braten, Miles N.; ...

2018-01-17

Nonaqueous redox flow batteries (NRFBs) represent an attractive technology for energy storage from intermittent renewable sources. In these batteries, electrical energy is stored in and extracted from electrolyte solutions of redox-active molecules (termed catholytes and anolytes) that are passed through an electrochemical flow cell. To avoid battery self-discharge, the anolyte and catholyte solutions must be separated by a membrane in the flow cell. This membrane prevents crossover of the redox active molecules, while simultaneously allowing facile transport of charge-balancing ions. A key unmet challenge for the field is the design of redox-active molecule/membrane pairs that enable effective electrolyte separation whilemore » maintaining optimal battery properties. Herein, we demonstrate the development of oligomeric catholytes based on tris(dialkylamino)cyclopropenium (CP) salts that are specifically tailored for pairing with size-exclusion membranes composed of polymers of intrinsic microporosity (PIMs). Systematic studies were conducted to evaluate the impact of oligomer size/structure on properties that are crucial for flow battery performance, including cycling stability, charge capacity, solubility, electron transfer kinetics, and crossover rates. These studies have led to the identification of a CP-derived tetramer in which these properties are all comparable, or significantly improved, relative to the monomeric counterpart. Finally, a proof-of-concept flow battery is demonstrated by pairing this tetrameric catholyte with a PIM membrane. After 6 days of cycling, no crossover is detected, demonstrating the promise of this approach. Finally, these studies provide a template for the future design of other redox-active oligomers for this application.« less

Labyrinthine flows across multilayer graphene-based membranes

NASA Astrophysics Data System (ADS)

Yoshida, Hiroaki

Graphene-based materials have recently found extremely wide applications for fluidic purposes thanks to remarkable developments in micro-/nano-fabrication techniques. In particular, high permeability and specific selectivity have been reported for these graphene-based membranes, such as the graphene-oxide membranes, with however controversial experimental results. There is therefore an urgent need to propose a theoretical framework of fluid transport in these architectures in order to rationalize the experimental results.In this presentation, we report a theoretical study of mass transport across multilayer graphene based membranes, which we benchmark by atomic-scale molecular dynamics. Specifically, we consider the water flow across multiple graphene layers with an inter-layer distance ranging from sub-nanometer to a few nanometers. The graphene layers have nanoslits aligned in a staggered fashion, and thus the water flows involve multiple twists and turns. We compare the continuum model predictions for the permeability with the lattice Boltzmann calculations and molecular dynamics simulations. The highlight is that, in spite of extreme confinement, the permeability across the graphene-based membrane is quantitatively predicted on the basis of a properly designed continuum model. The framework of this study constitutes a benchmark to which we compare favourably published experimental data.In addition, flow properties of a water-ethanol mixture are presented, demonstrating the possibility of a novel separation technique. While the membrane is permeable to both pure liquids, it exhibits a counter-intuitive ``self-semi-permeability'' to water in the presence of the mixture. This suggests a robust and versatile membrane-based separation method built on a pressure-driven reverse-osmosis process, which is considerably less energy consuming than distillation processes. The author acknowledges the ERC project Micromegas and the ANR projects BlueEnergy and Equip@Meso.

Determination of base-flow characteristics at selected streamflow-gaging stations on the Mad River, Ohio

USGS Publications Warehouse

Koltun, G.F.

1995-01-01

This report describes the results of a study to estimate characteristics of base flow and sustained ground-water discharge at five streamflow-gaging stations on the Mad River in Ohio. The five streamflow-gaging stations are located at Zanesfield, near Urbana, at St. Paris Pike (at Eagle City), near Springfield, and near Dayton. The median of the annual-mean base flows, determined by means of hydrograph separation, ranged from 0.64 (ft3/s)/mi2 (cubic feet per second per square mile) at Zanesfield to 0.74 (ft3/s)/mi2 at St. Paris Pike. The median percentage of annual total streamflow attributed to base flow ranged from 61.8 percent at Zanesfield to 76.1 percent near Urbana. Estimates of an upper limit (or threshold) at which base flows can be considered to be composed predominately of sustained ground-water discharge were made by constructing and analyzing base- flow-duration curves. The sustained ground-water discharges (base flows less than or equal to the estimated sustained ground-water-discharge thresholds) are assumed to originate from ground-water- flow systems that are minimally affected by seasonal climatic changes. The median sustained ground- water discharge ranged from 0.11 (ft3/s)/mi2 at Zanesfield to 0.26 (ft3/s)/mi2 at St. Paris Pike (at Eagle City) and near Springfield. The median sustained ground-water discharge, expressed as a percentage of the median annual-mean base flow, ranged from 17.2 percent at Zanesfield to 38.6 percent near Springfield.

Recycling of rare earth particle by mini-hydrocyclones.

PubMed

Yu, Jian-Feng; Fu, Jian; Cheng, Hao; Cui, Zhengwei

2017-03-01

Mini-hydrocyclones were applied to separate the fine rare earth particles from the suspensions. The effects of the flow rate, split ratio, and feed concentration on the total separation efficiency and grade separation efficiency were studied. The combined effects of the flow rate (1200-1600L/h), split ratio (20-60%) and concentration (0.6-1.0wt%) on the total separation efficiency in mini-hydrocyclones were investigated using a response surface methodology. The optimum operating parameters for a total separation efficiency of 92.5% were: feed flow rate=1406L/h, split ratio=20%, and feed concentration=1wt%. Copyright © 2016 Elsevier Ltd. All rights reserved.

Large perturbation flow field analysis and simulation for supersonic inlets

NASA Technical Reports Server (NTRS)

Varner, M. O.; Martindale, W. R.; Phares, W. J.; Kneile, K. R.; Adams, J. C., Jr.

1984-01-01

An analysis technique for simulation of supersonic mixed compression inlets with large flow field perturbations is presented. The approach is based upon a quasi-one-dimensional inviscid unsteady formulation which includes engineering models of unstart/restart, bleed, bypass, and geometry effects. Numerical solution of the governing time dependent equations of motion is accomplished through a shock capturing finite difference algorithm, of which five separate approaches are evaluated. Comparison with experimental supersonic wind tunnel data is presented to verify the present approach for a wide range of transient inlet flow conditions.

Acoustic black hole in a stationary hydrodynamic flow of microcavity polaritons.

PubMed

Nguyen, H S; Gerace, D; Carusotto, I; Sanvitto, D; Galopin, E; Lemaître, A; Sagnes, I; Bloch, J; Amo, A

2015-01-23

We report an experimental study of superfluid hydrodynamic effects in a one-dimensional polariton fluid flowing along a laterally patterned semiconductor microcavity and hitting a micron-sized engineered defect. At high excitation power, superfluid propagation effects are observed in the polariton dynamics; in particular, a sharp acoustic horizon is formed at the defect position, separating regions of sub- and supersonic flow. Our experimental findings are quantitatively reproduced by theoretical calculations based on a generalized Gross-Pitaevskii equation. Promising perspectives to observe Hawking radiation via photon correlation measurements are illustrated.

In-flight total forces, moments and static aeroelastic characteristics of an oblique-wing research airplane

NASA Technical Reports Server (NTRS)

Curry, R. E.; Sim, A. G.

1984-01-01

A low-speed flight investigation has provided total force and moment coefficients and aeroelastic effects for the AD-1 oblique-wing research airplane. The results were interpreted and compared with predictions that were based on wind tunnel data. An assessment has been made of the aeroelastic wing bending design criteria. Lateral-directional trim requirements caused by asymmetry were determined. At angles of attack near stall, flow visualization indicated viscous flow separation and spanwise vortex flow. These effects were also apparent in the force and moment data.

Appliance of Inertial Gas-Dynamic Separation of Gas-Dispersion Flows in the Curvilinear Convergent-Divergent Channels for Compressor Equipment Reliability Improvement

NASA Astrophysics Data System (ADS)

Liaposhchenko, O. O.; Sklabinskyi, V. I.; Zavialov, V. L.; Pavlenko, I. V.; Nastenko, O. V.; Demianenko, M. M.

2017-08-01

The new methods of vibration and inertial gas-dynamic separation of gas-condensate and dusty flows and the corresponding separation devices are proposed in order to avoid emergencies and premature wear of parts and components of the compressor equipment. The formation of the gas flow and disperse particles in the curvilinear convergent-divergent channels are investigated. The optimizing hydrodynamic profiling of a geometrical configuration of curvilinear separation channels with rigid and flexible walls of baffles is carried out.

Gas separation and bubble behavior at a woven screen

NASA Astrophysics Data System (ADS)

Conrath, Michael; Dreyer, Michael E.

Gas-liquid two phase flows are widespread and in many applications the separation of both phases is necessary. Chemical reactors, water treatment devices or gas-free delivery of liquids like propellant are only some of them. We study the performance of a woven metal screen in respect to its phase separation behavior under static and dynamic conditions. Beside hydraulic screen resistance and static bubble point, our study also comprises the bubble detachment from the screen upon gas breakthrough. Since a woven screen is essentially an array of identical pores, analogies to bubble detachment from a needle can be established. While the bubble point poses an upper limit for pressurized gas at a wetted screen to preclude gas breakthrough, the necessary pressure for growing bubbles to detach from the screen pores a lower limit when breakthrough is already in progress. Based on that inside, the dynamic bubble point effects were constituted that relate to a trapped bubble at such a screen in liquid flow. A trapped is caused to break through the screen by the flow-induced pressure drop across it. Our model includes axially symmetric bubble shapes, degree of coverage of the screen and bubble pressurization due to hydraulic losses in the rest of the circuit. We have built an experiment that consists of a Dutch Twilled woven screen made of stainless steel in a vertical acrylic glass tube. The liquid is silicon oil SF0.65. The screen is suspended perpendicular to the liquid flow which is forced through it at variable flow rate. Controlled injection of air from a needle allows us to examine the ability of the screen to separate gas and liquid along the former mentioned effects. We present experimental data on static bubble point and detachment pressure for breakthrough at different gas supply rates that suggest a useful criterion for reliable static bubble point measurements. Results for the dynamic bubble point are presented that include i) screen pressure drop for different trapped bubble volumes, liquid flow rates and flow-induced compression, ii) typical breakthrough of a trapped bubble at rising liquid flow rate and iii) steady gas supply in steady liquid flow. It shows that our model can explain the experimental observations. One of the interesting findings for the dynamic bubble point is that hydraulic losses in the rest of the circuit will shift the breakthrough of gas to higher liquid flow rates.

Experimental Investigation of Normal Shock Boundary-Layer Interaction with Hybrid Flow Control

NASA Technical Reports Server (NTRS)

Vyas, Manan A.; Hirt, Stefanie M.; Anderson, Bernhard H.

2012-01-01

Hybrid flow control, a combination of micro-ramps and micro-jets, was experimentally investigated in the 15x15 cm Supersonic Wind Tunnel (SWT) at the NASA Glenn Research Center. Full factorial, a design of experiments (DOE) method, was used to develop a test matrix with variables such as inter-ramp spacing, ramp height and chord length, and micro-jet injection flow ratio. A total of 17 configurations were tested with various parameters to meet the DOE criteria. In addition to boundary-layer measurements, oil flow visualization was used to qualitatively understand shock induced flow separation characteristics. The flow visualization showed the normal shock location, size of the separation, path of the downstream moving counter-rotating vortices, and corner flow effects. The results show that hybrid flow control demonstrates promise in reducing the size of shock boundary-layer interactions and resulting flow separation by means of energizing the boundary layer.