A Case of Alport Syndrome with Posttransplant Antiglomerular Basement Membrane Disease despite Negative Antiglomerular Basement Membrane Antibodies by EIA Treated with Plasmapheresis and Intravenous Immunoglobulin.

PubMed

Armstead, Sumiko I; Hellmark, Thomas; Wieslander, Jorgen; Zhou, Xin J; Saxena, Ramesh; Rajora, Nilum

2013-01-01

Posttransplant antiglomerular basement membrane (anti-GBM) disease occurs in approximately 5% of Alport patients and usually ends in irreversible graft failure. Recent research has focused on characterizing the structure of the anti-GBM alloepitope. Here we present a case of a 22-year-old male with end-stage renal disease secondary to Alport syndrome, with a previously failed renal allograft, who received a second deceased-donor kidney transplant. Six days after transplantation, he developed acute kidney injury. The serum anti-GBM IgG was negative by enzyme immunoassay (EIA). On biopsy, he had crescentic glomerulonephritis with linear GBM fixation of IgG. With further analysis by western blotting, we were able to detect antibodies to an unidentified protein from the basement membrane. This patient was treated with plasmapheresis twice per week and monthly intravenous immunoglobulin (IVIG) for a total of five months. At the end of treatment, these unknown antibodies were no longer detected. His renal function improved, and he has not required dialysis. We conclude that anti-GBM disease in patients with Alport Syndrome may be caused by circulating antibodies to other components of the basement membrane that are undetectable by routine anti-GBM EIA and may respond to treatment with plasmapheresis and IVIG.

Detection of Anti-basement Membrane Zone Antibodies in the Blister Fluid in Subepidermal Autoimmune Bullous Diseases

PubMed Central

Surya, Ravindran; Tejasvi, Bobbili; Shenoi, Shrutakirthi D; Pai, Sathish; Rao, Chythra; Rao, Raghavendra

2017-01-01

Background: Subepidermal autoimmune bullous diseases (sAIBD) are diverse of conditions with clinicopathological overlap. Circulating autoantibodies in the serum can be demonstrated using indirect immunofluorescence (IIF) microscopy. Artificially split normal human skin is considered as an optimum substrate for the demonstration of anti-basement membrane zone (BMZ) antibodies using IIF in sAIBD; it not only helps to detect the presence of circulating antibodies in the serum but also helps to subclassify these conditions into “roof” and “floor” binding disorders. Aim: In this study, we evaluated the utility of IIF to detect anti-BMZ antibodies in the blister fluid of patients with sAIBD. Materials and Methods: Twenty-two patients with a clinical diagnosis of sAIBD were enrolled in the study. IIF of serum and blister fluid were done simultaneously using salt-split skin as a substrate. Results: Anti-BMZ antibodies could be detected in the blister fluid using IIF in all patients in the study group. Limitation: We could not do enzyme-linked immunosorbent assay of blister fluid. This would have given us the quantitative data of circulating antibodies in the blister fluid. Conclusion: Blister fluid offers an alternate source for the detection of autoantibodies in patients with sAIBD. It may be of particular help in children and in elderly with poor venous access. PMID:29263541

Alport syndrome and Pierson syndrome: Diseases of the glomerular basement membrane.

PubMed

Funk, Steven D; Lin, Meei-Hua; Miner, Jeffrey H

2018-04-16

The glomerular basement membrane (GBM) is an important component of the kidney's glomerular filtration barrier. Like all basement membranes, the GBM contains type IV collagen, laminin, nidogen, and heparan sulfate proteoglycan. It is flanked by the podocytes and glomerular endothelial cells that both synthesize it and adhere to it. Mutations that affect the GBM's collagen α3α4α5(IV) components cause Alport syndrome (kidney disease with variable ear and eye defects) and its variants, including thin basement membrane nephropathy. Mutations in LAMB2 that impact the synthesis or function of laminin α5β2γ1 (LM-521) cause Pierson syndrome (congenital nephrotic syndrome with eye and neurological defects) and its less severe variants, including isolated congenital nephrotic syndrome. The very different types of kidney diseases that result from mutations in collagen IV vs. laminin are likely due to very different pathogenic mechanisms. A better understanding of these mechanisms should lead to targeted therapeutic approaches that can help people with these rare but important diseases. Copyright © 2017 International Society of Matrix Biology. Published by Elsevier B.V. All rights reserved.

Basement membrane of mouse bone marrow sinusoids shows distinctive structure and proteoglycan composition: a high resolution ultrastructural study.

PubMed

Inoue, S; Osmond, D G

2001-11-01

Venous sinusoids in bone marrow are the site of a large-scale traffic of cells between the extravascular hemopoietic compartment and the blood stream. The wall of the sinusoids consists solely of a basement membrane interposed between a layer of endothelial cells and an incomplete covering of adventitial cells. To examine its possible structural specialization, the basement membrane of bone marrow sinusoids has now been examined by high resolution electron microscopy of perfusion-fixed mouse bone marrow. The basement membrane layer was discontinuous, consisting of irregular masses of amorphous material within a uniform 60-nm-wide space between apposing endothelial cells and adventitial cell processes. At maximal magnifications, the material was resolved as a random arrangement of components lacking the "cord network" formation seen in basement membranes elsewhere. Individual components exhibited distinctive ultrastructural features whose molecular identity has previously been established. By these morphological criteria, the basement membrane contained unusually abundant chondroitin sulfate proteoglycan (CSPG) revealed by 3-nm-wide "double tracks," and moderate amounts of both laminin as dense irregular coils and type IV collagen as 1-1.5-nm-wide filaments, together with less conspicuous amounts of amyloid P forming pentagonal frames. In contrast, 4.5-5-nm-wide "double tracks" characteristic of heparan sulfate proteoglycan (HSPG) were absent. The findings demonstrate that, in comparison with "typical" basement membranes in other tissues, the bone marrow sinusoidal basement membrane is uniquely specialized in several respects. Its discontinuous nature, lack of network organization, and absence of HSPG, a molecule that normally helps to maintain membrane integrity, may facilitate disassembly and reassembly of basement membrane material in concert with movements of adventitial cell processes as maturing hemopoietic cells pass through the sinusoidal wall: the

Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease.

PubMed

Mak, Ki M; Mei, Rena

2017-08-01

Basement membranes provide structural support to epithelium, endothelium, muscles, fat cells, Schwann cells, and axons. Basement membranes are multifunctional: they modulate cellular behavior, regulate organogenesis, promote tissue repair, form a barrier to filtration and tumor metastasis, bind growth factors, and mediate angiogenesis. All basement membranes contain type IV collagen (Col IV), laminin, nidogen, and perlecan. Col IV and laminin self-assemble into two independent supramolecular networks that are linked to nidogen and perlecan to form a morphological discernable basement membrane/basal lamina. The triple helical region, 7S domain and NCI domain of Col IV, laminin and laminin fragment P1 have been evaluated as noninvasive fibrosis biomarkers of alcoholic liver disease, viral hepatitis, and nonalcoholic fatty liver disease. Elevated serum Col IV and laminin are related to degrees of fibrosis and severity of hepatitis, and may reflect hepatic basement membrane metabolism. But the serum assays have not been linked to disclosing the anatomical sites and lobular distribution of perisinusoidal basement membrane formation in the liver. Hepatic sinusoids normally lack a basement membrane, although Col IV is a normal matrix component of the space of Disse. In liver disease, laminin deposits in the space of Disse and codistributes with Col IV, forming a perisinusoidal basement membrane. Concomitantly, the sinusoidal endothelium loses its fenestrae and is transformed into vascular type endothelium. These changes lead to capillarization of hepatic sinusoids, a significant pathology that impairs hepatic function. Accordingly, codistribution of Col IV and laminin serves as histochemical marker of perisinusoidal basement membrane formation in liver disease. Anat Rec, 300:1371-1390, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Studies of the permeation properties of glomerular basement membrane: cross-linking renders glomerular basement membrane permeable to protein.

PubMed

Walton, H A; Byrne, J; Robinson, G B

1992-03-20

Cross-linking glomerular basement membrane (GBM) has been shown to render it more permeable to protein. Isolated pig GBM was cross-linked with dimethylmalonimidate which reacts selectively with lysine epsilon-NH2 groups or with glutaraldehyde, a less selective cross-linking agent. Studies of the ultrafiltration properties of these materials in vitro using cytochrome c, myoglobin, bovine serum albumin and immunoglobulin showed that cross-linking had markedly increased solvent and protein fluxes as compared with native membranes particularly at higher pressures. Filtration studies with serum demonstrated that the cross-linked membranes were more permeable to serum proteins. Thickness measurements under pressure indicated that cross-linked membrane was less compressed than native membrane as pressure was increased. Pore theory did not provide a suitable model for analysis of the results, but analysis of the results using the fibre-matrix hypothesis indicated that cross-linking had the effect of bundling together the fibres (type IV collagen) in the GBM matrix. The effect of cross-linking on filtration could be explained by a combination of contraction of the membrane, fibre bundling and increased rigidity compared with native membrane. Cross-linking of GBM might lead to long-term damage of the glomerular capillary wall in nephritis, so promoting proteinuria.

Regulation of the basement membrane by epithelia generated forces

NASA Astrophysics Data System (ADS)

Tanner, Kandice

2012-12-01

Tumor metastasis involves a progressive loss of tissue architecture and dissolution of structural boundaries between the epithelium and connective tissue. The basement membrane (BM), a specialized network of extracellular matrix proteins forms a barrier that physically restricts pre-invasive lesions such that they remain as local insults. The BM is not a static structure, but one that is constantly regenerated and remodeled in the adult organism. Matrix organization also regulates cell function. Thus alterations in the balance of synthesis, remodeling and proteolytic degradation of the extracellular matrix proteins may contribute to a loss of structural integrity. However, the de novo assembly and maintenance of the complex structural properties of in vivo basement membranes remain elusive. Here, this paper highlights the current understanding on the structural properties and the establishment of the BM, and discusses the potential role of self-generated forces in adult tissue remodeling and the maintenance of the BM as a malignancy suppressor.

Regeneration of the epidermis and basement membrane of the planarian Dugesia japonica after total-body x irradiation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hori, I.

1979-03-01

Fresh-water planarians were studied to examine effects of x rays on regeneration of the epidermis and basement membrane. During early stages of regeneration, free rhabdite-forming cells were associated with the wound epidermis and recruited it. In later stages, however, a gradual degeneration occurred in the epidermis and cells undergoing epithelization decreased in number. Eventually epidermal cells on the wound surface appeared necrotic as evidenced by pyknotic nuclei and vacuolized dense cytoplasm. The entire basement membrane could not be reconstituted in any stage after wounding though its precursor-like material was secreted in the interspace between epidermis and parenchyma. Morphological changes inmore » extracellular products and in the cells surrounding the products suggest that epidermal cells which have covered the wound surface synthesize precursors of the basement membrane. Possible factors of a characteristic perturbation in epithelization and basement membrane formation after total-body irradiation are discussed.« less

Collagen IV Diseases: A Focus on the Glomerular Basement Membrane in Alport Syndrome

PubMed Central

Cosgrove, Dominic; Liu, Shiguang

2016-01-01

Alport syndrome is the result of mutations in any of three type IV collagen genes, COL4A3, COL4A4, or COL4A5. Because the three collagen chains form heterotrimers, there is an absence of all three proteins in the basement membranes where they are expressed. In the glomerulus, the mature glomerular basement membrane type IV collagen network, normally comprised of two separate networks, α3(IV)/α4(IV)/α5(IV) and α1(IV)/α2(IV), is comprised entirely of collagen α1(IV)/α2. This review addresses the current state of our knowledge regarding the consequence of this change in basement membrane composition, including both the direct, via collagen receptor binding, and indirect, regarding influences on glomerular biomechanics. The state of our current understanding regarding mechanisms of glomerular disease initiation and progression will be examined, as will the current state of the art regarding emergent therapeutic approaches to slow or arrest glomerular disease in Alport patients. PMID:27576055

Ten cases of severe oral lichen planus showing granular C3 deposition in oral mucosal basement membrane zone.

PubMed

Hashimoto, Takashi; Fukuda, Aoi; Himejima, Akio; Morita, Shosuke; Tsuruta, Daisuke; Koga, Hiroshi; Krol, Rafal P; Ishii, Norito

2015-01-01

Oral lichen planus (OLP) may show depositions of immunoglobulins and complement components in oral mucosal basement membrane zone (BMZ) in direct immunofluorescence, although these finding are not frequently seen. We collected and examined ten cases of severe OLP showing granular C3 deposition in BMZ. In addition to clinical, histopathological and direct immunofluorescence assessments, we performed various immune-serological tests, including indirect immunofluorescence of normal human skin and 1M NaCl-split skin, immunoblotting of normal human epidermal and dermal extracts, recombinant proteins of BP180 NC16a and C-terminal domains, concentrated culture supernatant of HaCaT cells and purified human laminin-332, and enzyme-linked immunosorbent assays for BP230 and BP180. Direct immunofluorescence showed C3 deposition in BMZ exclusively of granular pattern in 7 cases and of both granular and linear patterns in 3 cases. The 10 cases showed no positive reactivity for either IgG or IgA antibodies in any immuno-serological tests. Detailed analyses of clinical, histopathological and immunological findings revealed striking female prevalence, although other parameters were in general characteristic of OLP. Granular C3 deposition in oral BMZ may be one of the characteristic features of severe OLP, although mechanisms for C3 deposition and its pathogenic role in OLP are currently unknown.

[Relationship between the changes in ischemia/reperfusion cerebro-microvessel basement membrane injury and gelatinase system in senile rat].

PubMed

Li, Jian-sheng; Liu, Ke; Liu, Jing-xia; Wang, Ming-hang; Zhao, Yue-wu; Liu, Zheng-guo

2008-11-01

To study the relationship of cerebro-microvessel basement membrane injury and gelatinase system after cerebral ischemia/reperfusion (I/R) in aged rats. Cerebral I/R injury model was reproduced by intraluminal silk ligature thrombosis of the middle cerebral artery occlusion (MCAO). Rats were divided randomly into sham control and I/R groups in young rats [ischemia 3 hours (I 3 h) and reperfusion 6 hours (I/R 6 h), 12 hours (I/R 12 h), 24 hours (I/R 24 h), 3 days (I/R 3 d), 6 days (I/R 6 d)], and sham control group and I/R group in aged rats (I 3 h and I/R 6 h, I/R 12 h, I/R 24 h , I/R 3 d, I/R 6 d). The change in cerebro-cortex microvessel basement membrane structure, basement membrane type IV collagen (Col IV) and laminin (LN) contents, matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) expression in every group were determined with immunohistochemical method and zymogram analysis. With the increase in age, Col IV and LN contents of the microvessel basement membrane were increased, and MMP-2 and MMP-9 expressions were stronger. With prolongation of I/R, the degradation of microvessel basement membrane components (Col IV and LN) was positively correlated with the duration of cerebral I/R. MMP-2 expression was increased gradually, and MMP-9 and TIMP-1 expression increased at the beginning and decreased subsequently. Col IV(I 3 h, I/R 6 h , I/R 12 h), LN (I 3 h, I/R 6-24 h), MMP-2 (I 3 h, I/R 6 h-6 d) and MMP-9 (I 3 h, I/R 6-24 h) expression level in aged rats with I/R injury were higher, and TIMP-1 (I/R 24 h) expression was lower than those in young rats (P<0.05 or P<0.01). In addition, changes in MMP-2 and MMP-9 contents as determined by zymogram analysis method coincided with their immunoexpression. With the increase of age, alteration in membrane components of cerebro-microvessel basement membrane in rats is related with MMPs and TIMP. Cerebro-microvessel basement membrane injury is more serious in aged rats than that of young rats

The timing of metamorphism in the Odenwald-Spessart basement, Mid-German Crystalline Zone

NASA Astrophysics Data System (ADS)

Will, T. M.; Schulz, B.; Schmädicke, E.

2017-07-01

New in situ electron microprobe monazite and white mica 40Ar/39Ar step heating ages support the proposition that the Odenwald-Spessart basement, Mid-German Crystalline Zone, consists of at least two distinct crustal terranes that experienced different geological histories prior to their juxtaposition. The monazite ages constrain tectonothermal events at 430 ± 43 Ma, 349 ± 14 Ma, 331 ± 16 Ma and 317 ± 12 Ma/316 ± 4 Ma, and the 40Ar/39Ar analyses provide white mica ages of 322 ± 3 Ma and 324 ± 3 Ma. Granulite-facies metamorphism occurred in the western Odenwald at c. 430 and 349 Ma, and amphibolite-facies metamorphism affected the eastern Odenwald and the central Spessart basements between c. 324 and 316 Ma. We interpret these data to indicate that the Otzberg-Michelbach Fault Zone, which separates the eastern Odenwald-Spessart basement from the Western Odenwald basement, is part of the Rheic Suture, which marks the position of a major Variscan plate boundary separating Gondwana- and Avalonia-derived crustal terranes. The age of the Carboniferous granulite-facies event in the western Odenwald overlaps with the minimum age of eclogite-facies metamorphism in the adjacent eastern Odenwald. The granulite- and eclogite-facies rocks experienced contrasting pressure-temperature paths but occur in close spatial proximity, being separated by the Rheic Suture. As high-pressure and high-temperature metamorphisms are of similar age, we interpret the Odenwald-Spessart basement as a paired metamorphic belt and propose that the adjacent high-pressure and high-temperature rocks were metamorphosed in the same subduction zone system. Juxtaposition of these rocks occurred during the final stages of the Variscan orogeny along the Rheic Suture.

Basement Membrane Defects in Genetic Kidney Diseases

PubMed Central

Chew, Christine; Lennon, Rachel

2018-01-01

The glomerular basement membrane (GBM) is a specialized structure with a significant role in maintaining the glomerular filtration barrier. This GBM is formed from the fusion of two basement membranes during development and its function in the filtration barrier is achieved by key extracellular matrix components including type IV collagen, laminins, nidogens, and heparan sulfate proteoglycans. The characteristics of specific matrix isoforms such as laminin-521 (α5β2γ1) and the α3α4α5 chain of type IV collagen are essential for the formation of a mature GBM and the restricted tissue distribution of these isoforms makes the GBM a unique structure. Detailed investigation of the GBM has been driven by the identification of inherited abnormalities in matrix proteins and the need to understand pathogenic mechanisms causing severe glomerular disease. A well-described hereditary GBM disease is Alport syndrome, associated with a progressive glomerular disease, hearing loss, and lens defects due to mutations in the genes COL4A3, COL4A4, or COL4A5. Other proteins associated with inherited diseases of the GBM include laminin β2 in Pierson syndrome and LMX1B in nail patella syndrome. The knowledge of these genetic mutations associated with GBM defects has enhanced our understanding of cell–matrix signaling pathways affected in glomerular disease. This review will address current knowledge of GBM-associated abnormalities and related signaling pathways, as well as discussing the advances toward disease-targeted therapies for patients with glomerular disease. PMID:29435440

Rituximab for the treatment of refractory simultaneous anti-glomerular basement membrane (anti-GBM) and membranous nephropathy.

PubMed

Bandak, Ghassan; Jones, Bruce A; Li, Jian; Yee, Jerry; Umanath, Kausik

2014-02-01

Antibody-mediated anti-glomerular basement membrane (anti-GBM) disease occurs rarely in the presence of another B-cell disorder, membranous nephropathy. The coexistence of these two autoimmune disorders would be anticipated to require differing, specific therapies targeted to each disease process. We describe a case of concomitant membranous nephropathy and anti-GBM disease in which conventional therapy, including steroids, plasmapheresis and cyclophosphamide, failed to attenuate the anti-GBM disease, yet responded to an alternative treatment of rituximab. This B-cell directed, monoclonal, chimeric antibody treatment substantially reduced anti-GBM antibody titers and led to discontinuation of plasmapheresis, while maintaining the remission of membranous nephropathy and anti-GBM disease.

Inhibition of laminin alpha 1-chain expression leads to alteration of basement membrane assembly and cell differentiation

PubMed Central

1996-01-01

The expression of the constituent alpha 1 chain of laminin-1, a major component of basement membranes, is markedly regulated during development and differentiation. We have designed an antisense RNA strategy to analyze the direct involvement of the alpha 1 chain in laminin assembly, basement membrane formation, and cell differentiation. We report that the absence of alpha 1-chain expression, resulting from the stable transfection of the human colonic cancer Caco2 cells with an eukaryotic expression vector comprising a cDNA fragment of the alpha 1 chain inserted in an antisense orientation, led to (a) an incorrect secretion of the two other constituent chains of laminin-1, the beta 1/gamma 1 chains, (b) the lack of basement membrane assembly when Caco2-deficient cells were cultured on top of fibroblasts, assessed by the absence of collagen IV and nidogen deposition, and (c) changes in the structural polarity of cells accompanied by the inhibition of an apical digestive enzyme, sucrase-isomaltase. The results demonstrate that the alpha 1 chain is required for secretion of laminin-1 and for the assembly of basement membrane network. Furthermore, expression of the laminin alpha 1-chain gene may be a regulatory element in determining cell differentiation. PMID:8609173

Difficulties in differentiating thin basement membrane disease from Alport syndrome.

PubMed

Żurawski, Jakub; Burchardt, Paweł; Seget, Monika; Moczko, Jerzy; Woźniak, Aldona; Grochowalski, Marcin; Salwa-Żurawska, Wiesława

We examined a group of 83 patients (57 children and 26 adults) with thin basement membrane disease and 17 patients with Alport syndrome. We compared the clinical data and, above all, the morphological patterns of both disease entities, with particular focus on not very advanced changes which might lead to a misdiagnosis due to the non-detection of the early stages of Alport syndrome.

Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors.

PubMed

Benedicto, Ignacio; Lehmann, Guillermo L; Ginsberg, Michael; Nolan, Daniel J; Bareja, Rohan; Elemento, Olivier; Salfati, Zelda; Alam, Nazia M; Prusky, Glen T; Llanos, Pierre; Rabbany, Sina Y; Maminishkis, Arvydas; Miller, Sheldon S; Rafii, Shahin; Rodriguez-Boulan, Enrique

2017-05-19

The outer blood-retina barrier is established through the coordinated terminal maturation of the retinal pigment epithelium (RPE), fenestrated choroid endothelial cells (ECs) and Bruch's membrane, a highly organized basement membrane that lies between both cell types. Here we study the contribution of choroid ECs to this process by comparing their gene expression profile before (P5) and after (P30) the critical postnatal period when mice acquire mature visual function. Transcriptome analyses show that expression of extracellular matrix-related genes changes dramatically over this period. Co-culture experiments support the existence of a novel regulatory pathway: ECs secrete factors that remodel RPE basement membrane, and integrin receptors sense these changes triggering Rho GTPase signals that modulate RPE tight junctions and enhance RPE barrier function. We anticipate our results will spawn a search for additional roles of choroid ECs in RPE physiology and disease.

Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors

PubMed Central

Benedicto, Ignacio; Lehmann, Guillermo L.; Ginsberg, Michael; Nolan, Daniel J.; Bareja, Rohan; Elemento, Olivier; Salfati, Zelda; Alam, Nazia M.; Prusky, Glen T.; Llanos, Pierre; Rabbany, Sina Y.; Maminishkis, Arvydas; Miller, Sheldon S.; Rafii, Shahin; Rodriguez-Boulan, Enrique

2017-01-01

The outer blood-retina barrier is established through the coordinated terminal maturation of the retinal pigment epithelium (RPE), fenestrated choroid endothelial cells (ECs) and Bruch's membrane, a highly organized basement membrane that lies between both cell types. Here we study the contribution of choroid ECs to this process by comparing their gene expression profile before (P5) and after (P30) the critical postnatal period when mice acquire mature visual function. Transcriptome analyses show that expression of extracellular matrix-related genes changes dramatically over this period. Co-culture experiments support the existence of a novel regulatory pathway: ECs secrete factors that remodel RPE basement membrane, and integrin receptors sense these changes triggering Rho GTPase signals that modulate RPE tight junctions and enhance RPE barrier function. We anticipate our results will spawn a search for additional roles of choroid ECs in RPE physiology and disease. PMID:28524846

ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice

PubMed Central

Jones, Frances E.; Bailey, Matthew A.; Murray, Lydia S.; Lu, Yinhui; McNeilly, Sarah; Schlötzer-Schrehardt, Ursula; Lennon, Rachel; Sado, Yoshikazu; Brownstein, David G.; Mullins, John J.; Kadler, Karl E.; Van Agtmael, Tom

2016-01-01

ABSTRACT Collagen IV is a major component of basement membranes, and mutations in COL4A1, which encodes collagen IV alpha chain 1, cause a multisystemic disease encompassing cerebrovascular, eye and kidney defects. However, COL4A1 renal disease remains poorly characterized and its pathomolecular mechanisms are unknown. We show that Col4a1 mutations in mice cause hypotension and renal disease, including proteinuria and defects in Bowman's capsule and the glomerular basement membrane, indicating a role for Col4a1 in glomerular filtration. Impaired sodium reabsorption in the loop of Henle and distal nephron despite elevated aldosterone levels indicates that tubular defects contribute to the hypotension, highlighting a novel role for the basement membrane in vascular homeostasis by modulation of the tubular response to aldosterone. Col4a1 mutations also cause diabetes insipidus, whereby the tubular defects lead to polyuria associated with medullary atrophy and a subsequent reduction in the ability to upregulate aquaporin 2 and concentrate urine. Moreover, haematuria, haemorrhage and vascular basement membrane defects confirm an important vascular component. Interestingly, although structural and compositional basement membrane defects occurred in the glomerulus and Bowman's capsule, no tubular basement membrane defects were detected. By contrast, medullary atrophy was associated with chronic ER stress, providing evidence for cell-type-dependent molecular mechanisms of Col4a1 mutations. These data show that both basement membrane defects and ER stress contribute to Col4a1 renal disease, which has important implications for the development of treatment strategies for collagenopathies. PMID:26839400

Removal of the basement membrane enhances corneal wound healing.

PubMed

Pal-Ghosh, Sonali; Pajoohesh-Ganji, Ahdeah; Tadvalkar, Gauri; Stepp, Mary Ann

2011-12-01

Recurrent corneal erosions are painful and put patients' vision at risk. Treatment typically begins with debridement of the area around the erosion site followed by more aggressive treatments. An in vivo mouse model has been developed that reproducibly induces recurrent epithelial erosions in wild-type mice spontaneously within two weeks after a single 1.5 mm corneal debridement wound created using a dulled-blade. This study was conducted to determine whether 1) inhibiting MMP9 function during healing after dulled-blade wounding impacts erosion development and 2) wounds made with a rotating-burr heal without erosions. Oral or topical inhibition of MMPs after dulled-blade wounding does not improve healing. Wounds made by rotating-burr heal with significantly fewer erosions than dulled-blade wounds. The localization of MMP9, β4 integrin and basement membrane proteins (LN332 and type VII collagen), immune cell influx, and reinnervation of the corneal nerves were compared after both wound types. Rotating-burr wounds remove the anterior basement membrane centrally but not at the periphery near the wound margin, induce more apoptosis of corneal stromal cells, and damage more stromal nerve fibers. Despite the fact that rotating-burr wounds do more damage to the cornea, fewer immune cells are recruited and significantly more wounds resolve completely. Copyright © 2011 Elsevier Ltd. All rights reserved.

Anti-glomerular basement membrane disease and dual positivity for antineutrophil cytoplasmic antibody in a patient with membranous nephropathy.

PubMed

Meisels, I S; Stillman, I E; Kuhlik, A B

1998-10-01

We present the case of a 50-year-old man who underwent kidney biopsy for nephrotic syndrome. In addition to a membranous pattern, anti-glomerular basement membrane (anti-GBM) staining was noted before manifestations of anti-GBM disease. Hematuria and renal failure ensued 2 weeks later. In addition, he had simultaneous circulating levels of anti-GBM antibody and both perinuclear (P-) and cytoplasmic (C-) antineutrophil cytoplasmic antibody (ANCA).

Immunohistochemical expression of basement membrane proteins of verrucous carcinoma of the oral mucosa.

PubMed

Arduino, Paolo G; Carrozzo, Marco; Pagano, Marco; Broccoletti, Roberto; Scully, Crispian; Gandolfo, Sergio

2010-06-01

Squamous cell carcinoma (SCC) of the oral cavity is an extremely invasive tumour of stratified squamous epithelium that spreads throughout degradation of the basement membrane (BM) and extra-cellular matrix. Oral verrucous carcinoma (VC) is a rare low-grade variant of oral SCC that penetrates into the subepithelial connective tissue. It also has a different clinical behaviour from classical oral SCC. We investigated the immunohistochemical expression of laminin, laminin-5, collagen IV and fibronectin in VC, severe epithelial dysplasia (SED) and SCC in order to analyse if the pattern of these molecules expression contributes to the differences in the biological behaviour of these diseases. The staining pattern of laminin was less intensive in SCC compared with SED and VC, and collagen IV expression was increased in VC compared with SED. Discontinuities of laminin, collagen IV and fibronectin were more evident in SED than in VC. This study indicates that VC has a biological behaviour different from SED or SCC, observable by immunohistochemistry in the BM zone.

Quantitative image analysis of laminin immunoreactivity in skin basement membrane irradiated with 1 GeV/nucleon iron particles

NASA Technical Reports Server (NTRS)

Costes, S.; Streuli, C. H.; Barcellos-Hoff, M. H.

2000-01-01

We previously reported that laminin immunoreactivity in mouse mammary epithelium is altered shortly after whole-body irradiation with 0.8 Gy from 600 MeV/nucleon iron ions but is unaffected after exposure to sparsely ionizing radiation. This observation led us to propose that the effect could be due to protein damage from the high ionization density of the ion tracks. If so, we predicted that it would be evident soon after radiation exposure in basement membranes of other tissues and would depend on ion fluence. To test this hypothesis, we used immunofluorescence, confocal laser scanning microscopy, and image segmentation techniques to quantify changes in the basement membrane of mouse skin epidermis. At 1 h after exposure to 1 GeV/nucleon iron ions with doses from 0.03 to 1.6 Gy, neither the visual appearance nor the mean pixel intensity of laminin in the basement membrane of mouse dorsal skin epidermis was altered compared to sham-irradiated tissue. This result does not support the hypothesis that particle traversal directly affects laminin protein integrity. However, the mean pixel intensity of laminin immunoreactivity was significantly decreased in epidermal basement membrane at 48 and 96 h after exposure to 0.8 Gy 1 GeV/nucleon iron ions. We confirmed this effect with two additional antibodies raised against affinity-purified laminin 1 and the E3 fragment of the long-arm of laminin 1. In contrast, collagen type IV, another component of the basement membrane, was unaffected. Our studies demonstrate quantitatively that densely ionizing radiation elicits changes in skin microenvironments distinct from those induced by sparsely ionizing radiation. Such effects may might contribute to the carcinogenic potential of densely ionizing radiation by altering cellular signaling cascades mediated by cell-extracellular matrix interactions.

Embryoid body attachment to reconstituted basement membrane induces a genetic program of epithelial differentiation via jun N-terminal kinase signaling.

PubMed

Ho, Hoang-Yen; Moffat, Ryan C; Patel, Rupal V; Awah, Franklin N; Baloue, Kaitrin; Crowe, David L

2010-09-01

Embryonic stem (ES) cells are derived from early stage mammalian embryos and have broad developmental potential. These cells can be manipulated experimentally to generate cells of multiple tissue types which could be important in treating human diseases. The ability to produce relevant amounts of these differentiated cell populations creates the basis for clinical interventions in tissue regeneration and repair. Understanding how embryonic stem cells differentiate also can reveal important insights into cell biology. A previously reported mouse embryonic stem cell model demonstrated that differentiated epithelial cells migrated out of embryoid bodies attached to reconstituted basement membrane. We used genomic technology to profile ES cell populations in order to understand the molecular mechanisms leading to epithelial differentiation. Cells with characteristics of cultured epithelium migrated from embryoid bodies attached to reconstituted basement membrane. However, cells that comprised embryoid bodies also rapidly lost ES cell-specific gene expression and expressed proteins characteristic of stratified epithelia within hours of attachment to basement membrane. Gene expression profiling of sorted cell populations revealed upregulation of the BMP/TGFbeta signaling pathway, which was not sufficient for epithelial differentiation in the absence of basement membrane attachment. Activation of c-jun N-terminal kinase 1 (JNK1) and increased expression of Jun family transcription factors was observed during epithelial differentiation of ES cells. Inhibition of JNK signaling completely blocked epithelial differentiation in this model, revealing a key mechanism by which ES cells adopt epithelial characteristics via basement membrane attachment. Copyright (c) 2010 Elsevier B.V. All rights reserved.

Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components.

PubMed

Berasaín, P; Goñi, F; McGonigle, S; Dowd, A; Dalton, J P; Frangione, B; Carmona, C

1997-02-01

The invasive stages of the parasitic trematode Fasciola hepatica release proteinases into the medium in which they are maintained. In this study, we investigated the interaction of F. hepatica excretory/secretory (E/S) products and 2 cysteine proteinases (CL1 and CL2) purified from these products with extracellular matrix and basement membrane macromolecules. Fasciola hepatica E/S products contained collagenolytic activity on fibrillar types I and III collagen as well as basement membrane type IV collagen. CL1 and CL2 were capable of degrading acid-soluble type III and type IV collagen but not insoluble type I collagen. In contrast, neither the E/S products nor the purified CL1 and CL2 showed elastinolytic activity. Fibronectin and laminin were degraded by E/S products and by CL1 and CL2. Sequence analysis of fibronectin degradation products showed that the fragments obtained corresponded to complete biologically active domains. These results indicate that the cysteine proteinases secreted by F. hepatica may be involved in the process of tissue invasion by the parasite.

basement reservoir geometry and properties

NASA Astrophysics Data System (ADS)

Walter, bastien; Geraud, yves; Diraison, marc

2017-04-01

Basement reservoirs are nowadays frequently investigated for deep-seated fluid resources (e.g. geothermal energy, groundwater, hydrocarbons). The term 'basement' generally refers to crystalline and metamorphic formations, where matrix porosity is negligible in fresh basement rocks. Geothermal production of such unconventional reservoirs is controlled by brittle structures and altered rock matrix, resulting of a combination of different tectonic, hydrothermal or weathering phenomena. This work aims to characterize the petro-structural and petrophysical properties of two basement surface analogue case studies in geological extensive setting (the Albert Lake rift in Uganda; the Ifni proximal margin of the South West Morocco Atlantic coast). Different datasets, using field structural study, geophysical acquisition and laboratory petrophysical measurements, were integrated to describe the multi-scale geometry of the porous network of such fractured and weathered basement formations. This study points out the multi-scale distribution of all the features constituting the reservoir, over ten orders of magnitude from the pluri-kilometric scale of the major tectonics structures to the infra-millimetric scale of the secondary micro-porosity of fractured and weathered basements units. Major fault zones, with relatively thick and impermeable fault core structures, control the 'compartmentalization' of the reservoir by dividing it into several structural blocks. The analysis of these fault zones highlights the necessity for the basement reservoirs to be characterized by a highly connected fault and fracture system, where structure intersections represent the main fluid drainage areas between and within the reservoir's structural blocks. The suitable fluid storage areas in these reservoirs correspond to the damage zone of all the fault structures developed during the tectonic evolution of the basement and the weathered units of the basement roof developed during pre

Live-cell confocal microscopy and quantitative 4D image analysis of anchor cell invasion through the basement membrane in C. elegans

PubMed Central

Kelley, Laura C.; Wang, Zheng; Hagedorn, Elliott J.; Wang, Lin; Shen, Wanqing; Lei, Shijun; Johnson, Sam A.; Sherwood, David R.

2018-01-01

Cell invasion through basement membrane (BM) barriers is crucial during development, leukocyte trafficking, and for the spread of cancer. Despite its importance in normal and diseased states, the mechanisms that direct invasion are poorly understood, in large part because of the inability to visualize dynamic cell-basement membrane interactions in vivo. This protocol describes multi-channel time-lapse confocal imaging of anchor cell invasion in live C. elegans. Methods presented include outline slide preparation and worm growth synchronization (15 min), mounting (20 min), image acquisition (20-180 min), image processing (20 min), and quantitative analysis (variable timing). Images acquired enable direct measurement of invasive dynamics including invadopodia formation, cell membrane protrusions, and BM removal. This protocol can be combined with genetic analysis, molecular activity probes, and optogenetic approaches to uncover molecular mechanisms underlying cell invasion. These methods can also be readily adapted for real-time analysis of cell migration, basement membrane turnover, and cell membrane dynamics by any worm laboratory. PMID:28880279

Role of 17 beta-estradiol on type IV collagen fibers volumetric density in the basement membrane of bladder wall.

PubMed

de Fraga, Rogerio; Dambros, Miriam; Miyaoka, Ricardo; Riccetto, Cássio Luís Zanettini; Palma, Paulo César Rodrigues

2007-10-01

The authors quantified the type IV collagen fibers volumetric density in the basement membrane of bladder wall of ovariectomized rats with and without estradiol replacement. This study was conducted on 40 Wistar rats (3 months old) randomly divided in 4 groups: group 1, remained intact (control); group 2, submitted to bilateral oophorectomy and daily replacement 4 weeks later of 17 beta-estradiol for 12 weeks; group 3, sham operated and daily replacement 4 weeks later of sesame oil for 12 weeks; and group 4, submitted to bilateral oophorectomy and killed after 12 weeks. It was used in immunohistochemistry evaluation using type IV collagen polyclonal antibody to stain the fibers on paraffin rat bladder sections. The M-42 stereological grid system was used to analyze the fibers. Ovariectomy had an increase effect on the volumetric density of the type IV collagen fibers in the basement membrane of rat bladder wall. Estradiol replacement in castrated animals demonstrated a significative difference in the stereological parameters when compared to the castrated group without hormonal replacement. Surgical castration performed on rats induced an increasing volumetric density of type IV collagen fibers in the basement membrane of rats bladder wall and the estradiol treatment had a significant effect in keeping a low volumetric density of type IV collagen fibers in the basement membrane of rats bladder wall.

Regeneration of defective epithelial basement membrane and restoration of corneal transparency

PubMed Central

Marino, Gustavo K.; Santhiago, Marcony R.; Santhanam, Abirami; Torricelli, Andre A. M.; Wilson, Steven E.

2018-01-01

PURPOSE To study regeneration of the normal ultrastructure of the epithelial basement membrane (EBM) in rabbit corneas that had -9D photorefractive keratectomy (PRK) and developed late haze (fibrosis) with restoration of transparency over one to four months after surgery and in corneas that had incisional wounds. METHODS Twenty-four rabbits had one of their eyes included into one of the two procedure groups (-9D PRK or nearly full-thickness incisional wounds), while the opposite eye serving as unwounded controls. All corneas were evaluated with slit lamp photos, transmission electron microscopy and immunohistochemistry for the myofibroblast marker alpha-smooth muscle actin and collagen type III. RESULTS In the ‘-9D PRK group’, corneas at one month after surgery had dense corneal haze and no evidence of regenerated EBM ultrastructure. By two months after surgery, however, small areas of stromal clearing began to appear within the confluent opacity (lacunae), and these corresponded to small islands of normally-regenerated EBM detected within larger area of the excimer laser-ablated zone with no evidence of normal EBM. By four months after surgery, the EBM was fully-regenerated and the corneal transparency was completely restored to the ablated zone. In the ‘Incisional wound group’, the two dense, linear corneal opacities were observed at one month after surgery and progressively faded by two and three months after surgery. The EBM ultrastructure was fully regenerated at the site of the incisions, including around epithelial plugs that extended into the stroma, by one month after surgery in all eyes. CONCLUSIONS In the rabbit model, spontaneous resolution of corneal fibrosis (haze) after high correction PRK is triggered by regeneration of EBM with normal ultrastructure in the excimer laser- ablated zone. Conversely, incisional wounds heal in rabbit corneas without the development of myofibroblasts because the EBM regenerates normally by one month after surgery

Late Vendian Complexes in the Structure of Metamorphic Basement of the Fore Range Zone, Greater Caucasus

NASA Astrophysics Data System (ADS)

Kamzolkin, V. A.; Latyshev, A. V.; Vidyapin, Yu. P.; Somin, M. L.; Smul'skaya, A. I.; Ivanov, S. D.

2018-05-01

The paper presents new data on the composition, age, and relationships (with host and overlying deposits) of intrusive rocks in the basement of the Fore Range zone (Greater Caucasus), in the Malaya Laba River Basin. The evolutionary features of intrusive units located within the Blyb metamorphic complex are described. It is shown for the first time that the lower levels of this complex are, in a structural sense, outcrops of the Late Vendian basement. The basement is composed of the Balkan Formation and a massif of quartz metadiorites that intrudes it; for the rocks of this massif, ages ranging from 549 ± 7.4 to 574.1 ± 6.7 Ma are obtained for three U-Pb datings by the SHRIMP-II method. The Herzyinan magmatic event is represented by a group of granodiorite intrusions penetrating the Blyb complex on a series of faults extending along its boundary with the Main Range zone. The obtained estimate for the U-Pb age of one of the intrusions (319 ± 3.8 Ma) corresponds to the end of the Serpukhovian stage of the Early Carboniferous.

A Novel Function for the nm23-Hl Gene: Overexpression in Human Breast Carcinoma Cells Leads to the Formation of Basement Membrane and Growth Arrest

DOE Office of Scientific and Technical Information (OSTI.GOV)

Howlett, Anthony R; Petersen, Ole W; Steeg, Patricia S

1994-01-01

We have developed a culture system using reconstituted basement membrane components in which normal human mammary epithelial cells exhibit several aspects of the development and differentiation process, including formation of acinar-like structures, production and basal deposition of basement membrane components, and production and apical secretion of sialomucins. Cell lines and cultures from human breast carcinomas failed to recapitulate this process. The data indicate the importance of cellular interactions with the basement membrane in the regulation of normal breast differentiation and, potentially, its loss in neoplasia. Our purpose was to use this assay to investigate the role of the putative metastasismore » suppressor gene nm23-H1 in mammary development and differentiation. The metastatic human breast carcinoma cell line MDA-MB-435, clones transfected with a control pCMVBamneo vector, and clones transfected with pCMVBamneo vector containing nm23-H1 complementary DNA (the latter of which exhibited a substantial reduction in spontaneous metastatic potential in vivo) were cultured within a reconstituted basement membrane. Clones were examined for formation of acinus-like spheres, deposition of basement membrane components, production of sialomucin, polarization, and growth arrest. In contrast to the parental cell line and control transfectants, MDA-MB-435 breast carcinoma cells overexpressing Nm23-H1 protein regained several aspects of the normal phenotype within reconstituted basement membrane. Nm23-H1 protein-positive cells formed organized acinus-like spheres, deposited the basement membrane components type IV collagen and, to some extent, laminin to the outside of the spheres, expressed sialomucin, and growth arrested. Growth arrest of Nm23-H1 protein-positive cells was preceded by and correlated with formation of a basement membrane, suggesting a causal relationship. The data indicate a previously unidentified cause-and-effect relationship between nm23

High resolution immunoelectron microscopic localization of functional domains of laminin, nidogen, and heparan sulfate proteoglycan in epithelial basement membrane of mouse cornea reveals different topological orientations.

PubMed

Schittny, J C; Timpl, R; Engel, J

1988-10-01

Thin and ultrathin cryosections of mouse cornea were labeled with affinity-purified antibodies directed against either laminin, its central segments (domain 1), the end of its long arm (domain 3), the end of one of its short arms (domain 4), nidogen, or low density heparan sulfate proteoglycan. All basement membrane proteins are detected by indirect immunofluorescence exclusively in the epithelial basement membrane, in Descemet's membrane, and in small amorphous plaques located in the stroma. Immunoelectron microscopy using the protein A-gold technique demonstrated laminin domain 1 and nidogen in a narrow segment of the lamina densa at the junction to the lamina lucida within the epithelial basement membrane. Domain 3 shows three preferred locations at both the cellular and stromal boundaries of the epithelial basement membrane and in its center. Domain 4 is located predominantly in the lamina lucida and the adjacent half of the lamina densa. The low density heparan sulfate proteoglycan is found all across the basement membrane showing a similar uniform distribution as with antibodies against the whole laminin molecule. In Descemet's membrane an even distribution was found with all these antibodies. It is concluded that within the epithelial basement membrane the center of the laminin molecule is located near the lamina densa/lamina lucida junction and that its long arm favors three major orientations. One is close to the cell surface indicating binding to a cell receptor, while the other two are directed to internal matrix structures. The apparent codistribution of laminin domain 1 and nidogen agrees with biochemical evidence that nidogen binds to this domain.

Comparing The Efficacy of Hematoxylin and Eosin, Periodic Acid Schiff and Fluorescent Periodic Acid Schiff-Acriflavine Techniques for Demonstration of Basement Membrane in Oral Lichen Planus: A Histochemical Study

PubMed Central

Pujar, Ashwini; Pereira, Treville; Tamgadge, Avinash; Bhalerao, Sudhir; Tamgadge, Sandhya

2015-01-01

Background: Basement membrane (BM) is a thick sheet of extracellular matrix molecules, upon which epithelial cells attach. Various immunohistochemical studies in the past have been carried out but these advanced staining techniques are expensive and not feasible in routine laboratories. Although hematoxylin and eosin (H-E) is very popular among pathologists for looking at biopsies, the method has some limitations. This is where special stains come handy. Aims and Objectives: The aim of the present study was to demonstrate and compare the efficacy of H-E, periodic acid Schiff (PAS) and fluorescent periodic acid–acriflavine staining techniques for the basement membrane and to establish a histochemical stain which could be cost effective, less time consuming, and unambiguous for observation of the basement membrane zone. Materials and Methods: A total number of 40 paraffin-embedded tissue sections of known basement membrane containing tissues including 10 – Normal oral mucosa (NOM) and 30 – oral lichen planus (OLP) were considered in the study. Four-micron-thick sections of each block were cut and stained with H-E stain, PAS and fluorescent periodic acid–acriflavine stain. Sections were evaluated by three oral pathologists independently for continuity, contrast and pattern. Results: Though all the three stains showed favorable features at different levels, acriflavine stain was better than the other stains in demonstrating BM continuity, contrast and also the pattern followed by PAS stain. Acriflavine stain was the better in demonstrating a fibrillar pattern of a BM. Acriflavine stains a BM distinctly and is less time consuming and easy to carry out using readily available dyes as compared to other stains. Conclusion: The continuity and contrast along with the homogenous pattern and the afibrillar pattern of the BM was better demonstrated by acriflavine followed by the PAS stain. PMID:26538690

The First Evidence of the Precambrian Basement in the Fore Range Zone of the Great Caucasus.

NASA Astrophysics Data System (ADS)

Latyshev, A.; Kamzolkin, V.; Vidjapin, Y.; Somin, M.; Ivanov, S.

2017-12-01

Within the Great Caucasus fold-thrust belt, the Fore Range zone has the most complicated structure, and the highest degree of metamorphism was found there. This zone consists of several salients with the different composition and the structural and metamorphic evolution. The largest Blyb salient includes the metamorphic basement covered by the pack of thrusts. According to the recent isotopic data the upper levels of the Blyb metamorphic complex (BMC) are supposed to be Middle-Paleozoic (Somin, 2011). We studied zircons from the granitic intrusions located in the metamorphic rocks of the BMC. The U-Pb dating (SHRIMP II, VSEGEI, Russia) of zircons from the large Balkan metadiorite massif yielded the ages of 549±7,4, 574,1±6,7, and 567,9±6,9 Ma. All studied zircons show the high Th/U ratios and likely have the magmatic origin. This data is the first confirmation of the presence of the Precambrian basement and Vendian magmatic activity in the Fore Range zone. Zircons from the Unnamed granodiorite massif from the south of the Blyb salient yielded the age of 319±3.8 Ma (the Early Carboniferous). This fact taken together with the low grade of metamorphism in this intrusion reveals the Late Paleozoic magmatic event in the Fore Range zone. We also suggest that the Precambrian basement of the BMC, including the Balkan intrusion, is covered by so-called Armovsky nappe. This is confirmed by the field data, Middle-Paleozoic U-Pb ages and the higher degree of metamorphism of the Armovsky gneisses and schists. Thus, the BMC is not uniform but includes the blocks of the different age and metamorphic grades. Finally, we measured the anisotropy of magnetic susceptibility (AMS) of the Balkan metadiorites. The axes of AMS ellipsoid fix the conditions of the north-east compression, as well as the strain field reconstructed from the macrostructures orientation, which corresponds to the thrusts propagation. Therefore, the emplacement of the Balkan massif happened before the thrust

Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa

PubMed Central

Watt, Stephen A.; Dayal, Jasbani H. S.; Wright, Sheila; Riddle, Megan; Pourreyron, Celine; McMillan, James R.; Kimble, Roy M.; Prisco, Marco; Gartner, Ulrike; Warbrick, Emma; McLean, W. H. Irwin; Leigh, Irene M.; McGrath, John A.; Salas-Alanis, Julio C.; Tolar, Jakub; South, Andrew P.

2015-01-01

Recessive dystrophic epidermolysis bullosa (RDEB) is caused by mutations in COL7A1 resulting in reduced or absent type VII collagen, aberrant anchoring fibril formation and subsequent dermal-epidermal fragility. Here, we identify a significant decrease in PLOD3 expression and its encoded protein, the collagen modifying enzyme lysyl hydroxylase 3 (LH3), in RDEB. We show abundant LH3 localising to the basement membrane in normal skin which is severely depleted in RDEB patient skin. We demonstrate expression is in-part regulated by endogenous type VII collagen and that, in agreement with previous studies, even small reductions in LH3 expression lead to significantly less secreted LH3 protein. Exogenous type VII collagen did not alter LH3 expression in cultured RDEB keratinocytes and we show that RDEB patients receiving bone marrow transplantation who demonstrate significant increase in type VII collagen do not show increased levels of LH3 at the basement membrane. Our data report a direct link between LH3 and endogenous type VII collagen expression concluding that reduction of LH3 at the basement membrane in patients with RDEB will likely have significant implications for disease progression and therapeutic intervention. PMID:26380979

Laminin- and basement membrane-polycaprolactone blend nanofibers as a scaffold for regenerative medicine.

PubMed

Neal, Rebekah A; Lenz, Steven M; Wang, Tiffany; Abebayehu, Daniel; Brooks, Benjamin P C; Ogle, Roy C; Botchwey, Edward A

2014-09-01

Mimicking one or more components of the basement membrane (BM) holds great promise for overcoming insufficiencies in tissue engineering therapies. We have electrospun laminin nanofibers (NFs) isolated from the murine Engelbreth-Holm Swarm (EHS) tumor and evaluated them as a scaffold for embryonic stem cell culture. Seeded human embryonic stem cells were found to better maintain their undifferentiated, colony environment when cultured on laminin NFs compared to laminin mats, with 75% remaining undifferentiated on NFs. Mouse embryonic stem cells cultured on 10% laminin-polycaprolactone (PCL) NFs maintained their colony formation for twice as long without passage compared to those on PCL or gelatin substrates. In addition, we have established a protocol for electrospinning reconstituted basement membrane aligned (RBM)-PCL NFs within 10° of angular deviation. Neuron-like PC12 cells show significantly greater attachment (p < 0.001) and percentage of neurite-extending cells in vitro on 10% RBM-PCL NFs when compared to 1% and 0% RBM-PCL NFs (p < 0.015 and p < 0.001, respectively). Together, these results implicate laminin- and RBM-PCL scaffolds as a promising biomimetic substrate for regenerative medicine applications.

Convective influx/glymphatic system: tracers injected into the CSF enter and leave the brain along separate periarterial basement membrane pathways.

PubMed

Albargothy, Nazira J; Johnston, David A; MacGregor-Sharp, Matthew; Weller, Roy O; Verma, Ajay; Hawkes, Cheryl A; Carare, Roxana O

2018-05-12

Tracers injected into CSF pass into the brain alongside arteries and out again. This has been recently termed the "glymphatic system" that proposes tracers enter the brain along periarterial "spaces" and leave the brain along the walls of veins. The object of the present study is to test the hypothesis that: (1) tracers from the CSF enter the cerebral cortex along pial-glial basement membranes as there are no perivascular "spaces" around cortical arteries, (2) tracers leave the brain along smooth muscle cell basement membranes that form the Intramural Peri-Arterial Drainage (IPAD) pathways for the elimination of interstitial fluid and solutes from the brain. 2 μL of 100 μM soluble, fluorescent fixable amyloid β (Aβ) were injected into the CSF of the cisterna magna of 6-10 and 24-30 month-old male mice and their brains were examined 5 and 30 min later. At 5 min, immunocytochemistry and confocal microscopy revealed Aβ on the outer aspects of cortical arteries colocalized with α-2 laminin in the pial-glial basement membranes. At 30 min, Aβ was colocalised with collagen IV in smooth muscle cell basement membranes in the walls of cortical arteries corresponding to the IPAD pathways. No evidence for drainage along the walls of veins was found. Measurements of the depth of penetration of tracer were taken from 11 regions of the brain. Maximum depths of penetration of tracer into the brain were achieved in the pons and caudoputamen. Conclusions drawn from the present study are that tracers injected into the CSF enter and leave the brain along separate periarterial basement membrane pathways. The exit route is along IPAD pathways in which Aβ accumulates in cerebral amyloid angiopathy (CAA) in Alzheimer's disease. Results from this study suggest that CSF may be a suitable route for delivery of therapies for neurological diseases, including CAA.

Detection of microinvasion in vulvar and cervical intraepithelial neoplasia using double immunostaining for cytokeratin and basement membrane components.

PubMed

Rush, Demaretta; Hyjek, Elizabeth; Baergen, Rebecca N; Ellenson, Lora H; Pirog, Edyta C

2005-06-01

Identification of early invasion in vulvar intraepithelial neoplasia 3 (VIN 3) and cervical intraepithelial neoplasia 3 (CIN 3) may be difficult with the use of routine hematoxylin-eosin staining. Presence of obscuring inflammation and tangential tissue sectioning are the most common diagnostic pitfalls. To examine the utility of double immunostaining for cytokeratin-collagen IV or cytokeratin-laminin in the detection of early invasion in VIN 3 and CIN 3. The study group consisted of 10 cases of "VIN 3, suspicious for invasion" and 10 cases of "CIN 3, suspicious for invasion." The negative control group consisted of VIN 3 (n = 15) and CIN 3 (n = 10). The positive control group consisted of cases of invasive vulvar carcinoma (n = 11) and invasive cervical carcinoma (n = 25). All cases were double immunostained for cytokeratin and collagen IV and, in a separate reaction, for cytokeratin and laminin. The continuity of the basement membrane and the presence of stromal invasion were assessed in the stained sections. The staining for collagen IV and laminin yielded identical results. A well-defined, continuous basement membrane was visualized in all cases of VIN 3 and CIN 3. A discontinuous or absent basement membrane was observed around the malignant cells on the invasive tumor front in all cases of vulvar and cervical carcinoma. In 2 of 10 cases of VIN 3, suspicious for invasion and in 4 of 10 cases of CIN 3, suspicious for invasion definitive foci of microinvasion were identified with the use of double immunostaining. A well-defined, continuous basement membrane was present in the remaining cases "suspicious for invasion." Double immunostaining for cytokeratin- collagen IV or cytokeratin-laminin is useful for evaluation of early invasion in equivocal cases of VIN 3 and CIN 3.

Coexistence of Anti-Glomerular Basement Membrane Glomerulonephritis and Membranous Nephropathy in a Female Patient with Preserved Renal Function.

PubMed

Ogawara, Aoi; Harada, Makoto; Ichikawa, Tohru; Fujii, Kazuaki; Ehara, Takashi; Kobayashi, Mamoru

2017-12-01

Renal prognosis for anti-glomerular basement membrane (GBM) glomerulonephritis is poor. The greater the amount of anti-GBM antibody binding the antigen (type IV collagen of the glomerular basement membrane), the greater the number of crescents that develop in glomeruli, resulting in progression of renal impairment. Immunofluorescence staining reveals linear IgG depositions on glomerular capillary walls. Membranous nephropathy (MN) is one of the most common causes of nephrotic syndrome in middle-aged to elderly patients. Immune complex is deposited in the sub-epithelial space of the glomerulus resulting in the development of a membranous lesion. Immunofluorescence staining reveals granular IgG depositions on glomerular capillary walls. Coexisting anti-GBM glomerulonephritis and MN are rare and, here we report a case of coexisting anti-GBM glomerulonephritis and MN with preserved renal function. There are some cases of coexisting anti-GBM glomerulonephritis and MN do not show severely decreased renal function. A 76-year-old Japanese woman presented with nephrotic syndrome, microscopic hematuria, and was positive for anti-GBM antibody. Kidney biopsy revealed linear and granular IgG depositions in glomerular capillary walls, crescent formations, and electron-dense deposits in the sub-epithelial space. She was diagnosed with anti-GBM glomerulonephritis and MN. Steroid and cyclosporine therapy achieved complete remission, and kidney function was preserved. In conclusion, coexisting anti-GBM glomerulonephritis and MN can have preserved renal function. IgG subclass of deposited anti-GBM antibody may be associated with the severity of anti-GBM glomerulonephritis. In addition, in the case of nephrotic syndrome with hematuria, we should consider the possibility of coexisting anti-GBM glomerulonephritis and MN.

Geoelectrical characterisation of basement aquifers: the case of Iberekodo, southwestern Nigeria

NASA Astrophysics Data System (ADS)

Aizebeokhai, Ahzegbobor P.; Oyeyemi, Kehinde D.

2018-03-01

Basement aquifers, which occur within the weathered and fractured zones of crystalline bedrocks, are important groundwater resources in tropical and subtropical regions. The development of basement aquifers is complex owing to their high spatial variability. Geophysical techniques are used to obtain information about the hydrologic characteristics of the weathered and fractured zones of the crystalline basement rocks, which relates to the occurrence of groundwater in the zones. The spatial distributions of these hydrologic characteristics are then used to map the spatial variability of the basement aquifers. Thus, knowledge of the spatial variability of basement aquifers is useful in siting wells and boreholes for optimal and perennial yield. Geoelectrical resistivity is one of the most widely used geophysical methods for assessing the spatial variability of the weathered and fractured zones in groundwater exploration efforts in basement complex terrains. The presented study focuses on combining vertical electrical sounding with two-dimensional (2D) geoelectrical resistivity imaging to characterise the weathered and fractured zones in a crystalline basement complex terrain in southwestern Nigeria. The basement aquifer was delineated, and the nature, extent and spatial variability of the delineated basement aquifer were assessed based on the spatial variability of the weathered and fractured zones. The study shows that a multiple-gradient array for 2D resistivity imaging is sensitive to vertical and near-surface stratigraphic features, which have hydrological implications. The integration of resistivity sounding with 2D geoelectrical resistivity imaging is efficient and enhances near-surface characterisation in basement complex terrain.

Synthesis and deposition of basement membrane proteins by primary brain capillary endothelial cells in a murine model of the blood-brain barrier.

PubMed

Thomsen, Maj Schneider; Birkelund, Svend; Burkhart, Annette; Stensballe, Allan; Moos, Torben

2017-03-01

The brain vascular basement membrane is important for both blood-brain barrier (BBB) development, stability, and barrier integrity and the contribution hereto from brain capillary endothelial cells (BCECs), pericytes, and astrocytes of the BBB is probably significant. The aim of this study was to analyse four different in vitro models of the murine BBB for expression and possible secretion of major basement membrane proteins from murine BCECs (mBCECs). mBCECs, pericytes and glial cells (mainly astrocytes and microglia) were prepared from brains of C57BL/6 mice. The mBCECs were grown as monoculture, in co-culture with pericytes or mixed glial cells, or as a triple-culture with both pericytes and mixed glial cells. The integrity of the BBB models was validated by measures of transendothelial electrical resistance (TEER) and passive permeability to mannitol. The expression of basement membrane proteins was analysed using RT-qPCR, mass spectrometry and immunocytochemistry. Co-culturing mBCECs with pericytes, mixed glial cells, or both significantly increased the TEER compared to the monoculture, and a low passive permeability was correlated with high TEER. The mBCECs expressed all major basement membrane proteins such as laminin-411, laminin-511, collagen [α1(IV)] 2 α2(IV), agrin, perlecan, and nidogen 1 and 2 in vitro. Increased expression of the laminin α5 subunit correlated with the addition of BBB-inducing factors (hydrocortisone, Ro 20-1724, and pCPT-cAMP), whereas increased expression of collagen IV α1 primarily correlated with increased levels of cAMP. In conclusion, BCECs cultured in vitro coherently form a BBB and express basement membrane proteins as a feature of maturation. Cover Image for this issue: doi: 10.1111/jnc.13789. © 2016 International Society for Neurochemistry.

C-O-H-N fluids circulations and graphite precipitation in reactivated Hudsonian shear zones during basement uplift of the Wollaston-Mudjatik Transition Zone: Example of the Cigar Lake U deposit

NASA Astrophysics Data System (ADS)

Martz, Pierre; Cathelineau, Michel; Mercadier, Julien; Boiron, Marie-Christine; Jaguin, Justine; Tarantola, Alexandre; Demacon, Mickael; Gerbeaud, Olivier; Quirt, David; Doney, Amber; Ledru, Patrick

2017-12-01

Graphitic shear zones are spatially associated with unconformity-related uranium deposits that are located around the unconformity between the strata of the Paleo- to Mesoproterozoic Athabasca Basin (Saskatchewan, Canada) and its underlying Archean to Paleoproterozoic basement. The present study focuses on basement-hosted ductile-brittle graphitic shear zones near the Cigar Lake U deposit, one of the largest unconformity-related U deposits. The goal of the study is to decipher the pre-Athabasca Basin fluid migration history recorded within such structures and its potential role on the formation of such exceptional deposit. Dominantly C-O-H(-N) metamorphic fluids have been trapped in Fluid Inclusion Planes (FIPs) in magmatic quartz within ductile-brittle graphitic shear zones active during retrograde metamorphism associated with the formation of the Wollaston-Mudjatik Transition Zone (WMTZ) between ca. 1805 and 1720 Ma. Such fluids show a compositional evolution along the retrograde path, from a dense and pure CO2 fluid during the earliest stages, through a lower density CO2 ± CH4-N2 (± H2O) fluid and, finally, to a very low density CH4-N2 fluid. Statistical study of the orientation, distribution, proportion, and chemical characterization of the FIPs shows that: i) CO2 (δ13CCO2 around - 9‰ PDB) from decarbonation reactions and/or partial water-metamorphic graphite equilibrium initially migrated regionally and pervasively under lithostatic conditions at about 500 to 800 °C and 150 to 300 MPa. Such P-T conditions attest to a high geothermal gradient of around 60 to 90 °C/km, probably related to rapid exhumation of the basement or a large-scale heat source. ii) Later brittle reactivation of the shear zone at around 450 °C and 25-50 MPa favored circulation of CO2-CH4-N2(± H2O) fluids in equilibrium with metamorphic graphite (δ13CCO2 around - 14‰) under hydrostatic conditions and only within the shear zones. Cooling of these fluids and the water uptake linked

An Overlapping Case of Alport Syndrome and Thin Basement Membrane Disease.

PubMed

Alganabi, Mashriq; Eter, Ahmad

2016-10-01

We report a case of a 48-year-old male who presented with hematuria of at least 10 years, and has a daughter with hematuria as well. The patient has a history of degenerative hearing loss, decreased vision and cataract formation, but no diabetes, hypertension or proteinuria. A full serology and urology workup was negative for any abnormality. A kidney biopsy for the patient revealed a diagnosis of Alport syndrome but was unable to rule out thin basement membrane disease. The biopsy was inconclusive in making the diagnosis but the patient's clinical presentation led to the diagnosis of Alport syndrome. The patient's 10-year-old daughter also has hematuria with no clear etiology but now can subsequently be anticipatorily managed for Alport syndrome progression. Due to the rarity of the disease, diagnosis is often missed or delayed by primary care providers especially when no associated proteinuria has yet developed. This can lead to confusion and misdiagnosis with thin basement membrane disease, a generally benign hematuria without kidney failure progression. Additionally, biopsy can be inconclusive in these patients, relying on the physician's history and physical examination findings to diagnose. It is important to appropriately diagnose Alport syndrome not only to manage the patient's rate of kidney failure progression but also allow for a higher degree of suspicion, screening and intervention in the patient's family members. Both the inconclusive nature of kidney biopsies and the usefulness of diagnosis for family member screening are often overlooked in medical literature but are explored in this case.

An Overlapping Case of Alport Syndrome and Thin Basement Membrane Disease

PubMed Central

Alganabi, Mashriq; Eter, Ahmad

2016-01-01

We report a case of a 48-year-old male who presented with hematuria of at least 10 years, and has a daughter with hematuria as well. The patient has a history of degenerative hearing loss, decreased vision and cataract formation, but no diabetes, hypertension or proteinuria. A full serology and urology workup was negative for any abnormality. A kidney biopsy for the patient revealed a diagnosis of Alport syndrome but was unable to rule out thin basement membrane disease. The biopsy was inconclusive in making the diagnosis but the patient’s clinical presentation led to the diagnosis of Alport syndrome. The patient’s 10-year-old daughter also has hematuria with no clear etiology but now can subsequently be anticipatorily managed for Alport syndrome progression. Due to the rarity of the disease, diagnosis is often missed or delayed by primary care providers especially when no associated proteinuria has yet developed. This can lead to confusion and misdiagnosis with thin basement membrane disease, a generally benign hematuria without kidney failure progression. Additionally, biopsy can be inconclusive in these patients, relying on the physician’s history and physical examination findings to diagnose. It is important to appropriately diagnose Alport syndrome not only to manage the patient’s rate of kidney failure progression but also allow for a higher degree of suspicion, screening and intervention in the patient’s family members. Both the inconclusive nature of kidney biopsies and the usefulness of diagnosis for family member screening are often overlooked in medical literature but are explored in this case. PMID:27635185

Expert guidelines for the management of Alport syndrome and thin basement membrane nephropathy.

PubMed

Savige, Judy; Gregory, Martin; Gross, Oliver; Kashtan, Clifford; Ding, Jie; Flinter, Frances

2013-02-01

Few prospective, randomized controlled clinical trials address the diagnosis and management of patients with Alport syndrome or thin basement membrane nephropathy. Adult and pediatric nephrologists and geneticists from four continents whose clinical practice focuses on these conditions have developed the following guidelines. The 18 recommendations are based on Level D (Expert opinion without explicit critical appraisal, or based on physiology, bench research, or first principles-National Health Service category) or Level III (Opinions of respected authorities, based on clinical experience, descriptive studies, or reports of expert committees-U.S. Preventive Services Task Force) evidence. The recommendations include the use of genetic testing as the gold standard for the diagnosis of Alport syndrome and the demonstration of its mode of inheritance; the need to identify and follow all affected members of a family with X-linked Alport syndrome, including most mothers of affected males; the treatment of males with X-linked Alport syndrome and individuals with autosomal recessive disease with renin-angiotensin system blockade, possibly even before the onset of proteinuria; discouraging the affected mothers of males with X-linked Alport syndrome from renal donation because of their own risk of kidney failure; and consideration of genetic testing to exclude X-linked Alport syndrome in some individuals with thin basement membrane nephropathy. The authors recognize that as evidence emerges, including data from patient registries, these guidelines will evolve further.

Study of the relationship between mononuclear inflammatory infiltrate and Ki-67 and basement membrane and extracellular matrix protein expression in radicular cysts.

PubMed

Mourão, R V C; Júnior, E C Pinheiro; Barros Silva, P G; Turatti, E; Mota, M R L; Alves, A P N N

2016-05-01

To evaluate the relationship between mononuclear inflammatory infiltrate and the expression of a proliferative immunomarker (Ki-67) as well as to evaluate basement membrane and extracellular matrix proteins (laminin and collagen type IV) in radicular cysts and dentigerous cysts (DC). Immunohistochemical analyses were performed in heavily inflamed radicular cysts (HIRC), slightly inflamed radicular cysts (SIRC) and DC (n = 20) using Ki-67 (Dako(®) , 1 : 50), anticollagen type IV (DBS(®) , 1 : 40) and antilaminin (DBS(®) , 1 : 20). The data were analysed using anova/Tukey's test (Ki-67) and Kruskal-Wallis/Dunn's test (collagen type IV and laminin) (P < 0.05). The immunoexpression of Ki-67 was significantly greater in the SIRC group compared with the HIRC and DC (P = 0.0040). Likewise, the immunoexpression of collagen type IV in the basement membrane of the SIRC group was significantly more continuous (P = 0.0475) than in the HIRC group. DC had significantly less collagen type IV in extracellular matrix immunoexpression than HIRC and SIRC (P = 0.0246). Laminin was absent in the basement membrane in the SIRC and DC groups, and the extracellular matrix of the HIRC was weak and punctate. The presence of inflammatory factors in the radicular cyst wall modified the expression of proliferation factors in the epithelial lining and the expression of collagen type IV and laminin in the basement membrane, but did not modify extracellular matrix behaviour in radicular cysts. © 2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.

The interplay of fold mechanisms and basement weaknesses at the transition between Laramide basement-involved arches, north-central Wyoming, USA

NASA Astrophysics Data System (ADS)

Neely, Thomas G.; Erslev, Eric A.

2009-09-01

Horizontally-shortened, basement-involved foreland orogens commonly exhibit anastomosing networks of bifurcating basement highs (here called arches) whose structural culminations are linked by complex transition zones of diversely-oriented faults and folds. The 3D geometry and kinematics of the southern Beartooth arch transition zone of north-central Wyoming were studied to understand the fold mechanisms and control on basement-involved arches. Data from 1581 slickensided minor faults are consistent with a single regional shortening direction of 065°. Evidence for oblique-slip, vertical axis rotations and stress refraction at anomalously-oriented folds suggests formation over reactivated pre-existing weaknesses. Restorable cross-sections and 3D surfaces, constrained by surface, well, and seismic data, document blind, ENE-directed basement thrusting and associated thin-skinned backthrusting and folding along the Beartooth and Oregon Basin fault systems. Between these systems, the basement-cored Rattlesnake Mountain backthrust followed basement weaknesses and rotated a basement chip toward the basin before the ENE-directed Line Creek fault system broke through and connected the Beartooth and Oregon Basin fault systems. Slip was transferred at the terminations of the Rattlesnake Mountain fault block by pivoting to the north and tear faulting to the south. In summary, unidirectional Laramide compression and pre-existing basement weaknesses combined with fault-propagation and rotational fault-bend folding to create an irregular yet continuous basement arch transition.

Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo

PubMed Central

Klueh, Ulrike; Qiao, Yi; Czajkowski, Caroline; Ludzinska, Izabela; Antar, Omar; Kreutzer, Donald L.

2015-01-01

Background: Implantable glucose sensors demonstrate a rapid decline in function that is likely due to biofouling of the sensor. Previous efforts directed at overcoming this issue has generally focused on the use of synthetic polymer coatings, with little apparent effect in vivo, clearly a novel approach is required. We believe that the key to extending sensor life span in vivo is the development of biocompatible basement membrane (BM) based bio-hydrogels as coatings for glucose sensors. Method: BM based bio-hydrogel sensor coatings were developed using purified BM preparations (ie, Cultrex from Trevigen Inc). Modified Abbott sensors were coated with Cultrex BM extracts. Sensor performance was evaluated for the impact of these coatings in vitro and in vivo in a continuous glucose monitoring (CGM) mouse model. In vivo sensor function was assessed over a 28-day time period expressed as mean absolute relative difference (MARD) values. Tissue reactivity of both Cultrex coated and uncoated glucose sensors was evaluated at 7, 14, 21 and 28 days post–sensor implantation with standard histological techniques. Results: The data demonstrate that Cultrex-based sensor coatings had no effect on glucose sensor function in vitro. In vivo glucose sensor performance was enhanced following BM coating as determined by MARD analysis, particularly in weeks 2 and 3. In vivo studies also demonstrated that Cultrex coatings significantly decreased sensor-induced tissue reactions at the sensor implantation sites. Conclusion: Basement-membrane-based sensor coatings enhance glucose sensor function in vivo, by minimizing or preventing sensor-induced tissues reactions. PMID:26306494

Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo.

PubMed

Klueh, Ulrike; Qiao, Yi; Czajkowski, Caroline; Ludzinska, Izabela; Antar, Omar; Kreutzer, Donald L

2015-08-25

Implantable glucose sensors demonstrate a rapid decline in function that is likely due to biofouling of the sensor. Previous efforts directed at overcoming this issue has generally focused on the use of synthetic polymer coatings, with little apparent effect in vivo, clearly a novel approach is required. We believe that the key to extending sensor life span in vivo is the development of biocompatible basement membrane (BM) based bio-hydrogels as coatings for glucose sensors. BM based bio-hydrogel sensor coatings were developed using purified BM preparations (ie, Cultrex from Trevigen Inc). Modified Abbott sensors were coated with Cultrex BM extracts. Sensor performance was evaluated for the impact of these coatings in vitro and in vivo in a continuous glucose monitoring (CGM) mouse model. In vivo sensor function was assessed over a 28-day time period expressed as mean absolute relative difference (MARD) values. Tissue reactivity of both Cultrex coated and uncoated glucose sensors was evaluated at 7, 14, 21 and 28 days post-sensor implantation with standard histological techniques. The data demonstrate that Cultrex-based sensor coatings had no effect on glucose sensor function in vitro. In vivo glucose sensor performance was enhanced following BM coating as determined by MARD analysis, particularly in weeks 2 and 3. In vivo studies also demonstrated that Cultrex coatings significantly decreased sensor-induced tissue reactions at the sensor implantation sites. Basement-membrane-based sensor coatings enhance glucose sensor function in vivo, by minimizing or preventing sensor-induced tissues reactions. © 2015 Diabetes Technology Society.

Agrin is a major heparan sulfate proteoglycan in the human glomerular basement membrane.

PubMed

Groffen, A J; Ruegg, M A; Dijkman, H; van de Velden, T J; Buskens, C A; van den Born, J; Assmann, K J; Monnens, L A; Veerkamp, J H; van den Heuvel, L P

1998-01-01

Agrin is a heparan sulfate proteoglycan (HSPG) that is highly concentrated in the synaptic basal lamina at the neuromuscular junction (NMJ). Agrin-like immunoreactivity is also detected outside the NMJ. Here we show that agrin is a major HSPG component of the human glomerular basement membrane (GBM). This is in addition to perlecan, a previously characterized HSPG of basement membranes. Antibodies against agrin and against an unidentified GBM HSPG produced a strong staining of the GBM and the NMJ, different from that observed with anti-perlecan antibodies. In addition, anti-agrin antisera recognized purified GBM HSPG and competed with an anti-GBM HSPG monoclonal antibody in ELISA. Furthermore, both antibodies recognized a molecule that migrated in SDS-PAGE as a smear and had a molecular mass of approximately 200-210 kD after deglycosylation. In immunoelectron microscopy, agrin showed a linear distribution along the GBM and was present throughout the width of the GBM. This was again different from perlecan, which was exclusively present on the endothelial side of the GBM and was distributed in a nonlinear manner. Quantitative ELISA showed that, compared with perlecan, the agrin-like GBM HSPG showed a sixfold higher molarity in crude glomerular extract. These results show that agrin is a major component of the GBM, indicating that it may play a role in renal ultrafiltration and cell matrix interaction. (J Histochem Cytochem 46:19-27, 1998)

Precambrian basement geologic map of Montana; an interpretation of aeromagnetic anomalies

USGS Publications Warehouse

Sims, P.K.; O'Neill, J. M.; Bankey, Viki; Anderson, E.

2004-01-01

Newly compiled aeromagnetic anomaly data of Montana, in conjunction with the known geologic framework of basement rocks, have been combined to produce a new interpretive geologic basement map of Montana. Crystalline basement rocks compose the basement, but are exposed only in the cores of mountain ranges in southwestern Montana. Principal features deduced from the map are: (1) A prominent northeast-trending, 200-km-wide zone of spaced negative anomalies, which extends more than 700 km from southwestern Montana's Beaverhead Mountains to the Canadian border and reflects suturing of the Archean Mexican Hat Block against the Archean Wyoming Province along the Paleoproterozoic Trans-Montana Orogen (new name) at about 1.9-1.8 Ga; (2) North-northwest-trending magnetic lows in northeastern Montana, which reflect the 1.9-1.8 Ga Trans-Hudson Orogen and truncate the older Trans-Montana Zone; and (3) Subtle northwest- and west-trending negative anomalies in central and western Montana, which represent the northernmost segment of brittle-ductile transcurrent faults of the newly recognized Mesoproterozoic Trans-Rocky Mountain fault system. Structures developed in the Proterozoic provided zones of crustal weakness reactivated during younger Proterozoic and Phanerozoic igneous and tectonic activity. For example, the Trans-Montana Zone guided basement involved thrust faulting in southwestern Montana during the Sevier Orogeny. The Boulder Batholith and associated ore deposits and the linear belt of alkaline intrusions to the northeast were localized along a zone of weakness between the Missouri River suture and the Dillon shear zone of the Trans-Montana Orogen. The northwest-trending faults of Trans-Rocky Mountain system outline depocenters for sedimentary rocks in the Belt Basin. This fault system provided zones of weakness that guided Laramide uplifts during basement crustal shortening. Northwest-trending zones have been locally reactivated during Neogene basin-range extension.

Cell therapy for basement membrane-linked diseases.

PubMed

Nyström, Alexander; Bornert, Olivier; Kühl, Tobias

2017-01-01

For most disorders caused by mutations in genes encoding basement membrane (BM) proteins, there are at present only limited treatment options available. Genetic BM-linked disorders can be viewed as especially suited for treatment with cell-based therapy approaches because the proteins that need to be restored are located in the extracellular space. In consequence, complete and permanent engraftment of cells does not necessarily have to occur to achieve substantial causal therapeutic effects. For these disorders cells can be used as transient vehicles for protein replacement. In addition, it is becoming evident that BM-linked genetic disorders are modified by secondary diseases mechanisms. Cell-based therapies have also the ability to target such disease modifying mechanisms. Thus, cell therapies can simultaneously provide causal treatment and symptomatic relief, and accordingly hold great potential for treatment of BM-linked disorders. However, this potential has for most applications and diseases so far not been realized. Here, we will present the state of cell therapies for BM-linked diseases. We will discuss use of both pluripotent and differentiated cells, the limitation of the approaches, their challenges, and the way forward to potential wider implementation of cell therapies in the clinics. Copyright © 2016 Elsevier B.V. All rights reserved.

Nanoscale protein architecture of the kidney glomerular basement membrane

PubMed Central

Suleiman, Hani; Zhang, Lei; Roth, Robyn; Heuser, John E; Miner, Jeffrey H; Shaw, Andrey S; Dani, Adish

2013-01-01

In multicellular organisms, proteins of the extracellular matrix (ECM) play structural and functional roles in essentially all organs, so understanding ECM protein organization in health and disease remains an important goal. Here, we used sub-diffraction resolution stochastic optical reconstruction microscopy (STORM) to resolve the in situ molecular organization of proteins within the kidney glomerular basement membrane (GBM), an essential mediator of glomerular ultrafiltration. Using multichannel STORM and STORM-electron microscopy correlation, we constructed a molecular reference frame that revealed a laminar organization of ECM proteins within the GBM. Separate analyses of domains near the N- and C-termini of agrin, laminin, and collagen IV in mouse and human GBM revealed a highly oriented macromolecular organization. Our analysis also revealed disruptions in this GBM architecture in a mouse model of Alport syndrome. These results provide the first nanoscopic glimpse into the organization of a complex ECM. DOI: http://dx.doi.org/10.7554/eLife.01149.001 PMID:24137544

In vivo laser confocal microscopy findings in patients with map-dot-fingerprint (epithelial basement membrane) dystrophy.

PubMed

Kobayashi, Akira; Yokogawa, Hideaki; Sugiyama, Kazuhisa

2012-01-01

The purpose of this study was to investigate pathological changes of the corneal cell layer in patients with map-dot-fingerprint (epithelial basement membrane) dystrophy by in vivo laser corneal confocal microscopy. Two patients were evaluated using a cornea-specific in vivo laser scanning confocal microscope (Heidelberg Retina Tomograph 2 Rostock Cornea Module, HRT 2-RCM). The affected corneal areas of both patients were examined. Image analysis was performed to identify corneal epithelial and stromal deposits correlated with this dystrophy. Variously shaped (linear, multilaminar, curvilinear, ring-shape, geographic) highly reflective materials were observed in the "map" area, mainly in the basal epithelial cell layer. In "fingerprint" lesions, multiple linear and curvilinear hyporeflective lines were observed. Additionally, in the affected corneas, infiltration of possible Langerhans cells and other inflammatory cells was observed as highly reflective Langerhans cell-like or dot images. Finally, needle-shaped materials were observed in one patient. HRT 2-RCM laser confocal microscopy is capable of identifying corneal microstructural changes related to map-dot-fingerprint corneal dystrophy in vivo. The technique may be useful in elucidating the pathogenesis and natural course of map-dot-fingerprint corneal dystrophy and other similar basement membrane abnormalities.

Overexpression of β1-chain-containing laminins in capillary basement membranes of human breast cancer and its metastases

PubMed Central

Fujita, Manabu; Khazenzon, Natalya M; Bose, Shikha; Sekiguchi, Kiyotoshi; Sasaki, Takako; Carter, William G; Ljubimov, Alexander V; Black, Keith L; Ljubimova, Julia Y

2005-01-01

Introduction Laminins are the major components of vascular and parenchymal basement membranes. We previously documented a switch in the expression of vascular laminins containing the α4 chain from predominantly laminin-9 (α4β2γ1) to predominantly laminin-8 (α4β1γ1) during progression of human brain gliomas to high-grade glioblastoma multiforme. Here, differential expression of laminins was studied in blood vessels and ductal epithelium of the breast. Method In the present study the expressions of laminin isoforms α1–α5, β1–β3, γ1, and γ2 were examined during progression of breast cancer. Forty-five clinical samples of breast tissues including normal breast, ductal carcinomas in situ, invasive ductal carcinomas, and their metastases to the brain were compared using Western blot analysis and immunohistochemistry for various chains of laminin, in particular laminin-8 and laminin-9. Results Laminin α4 chain was observed in vascular basement membranes of most studied tissues, with the highest expression in metastases. At the same time, the expression of laminin β2 chain (a constituent of laminin-9) was mostly seen in normal breast and carcinomas in situ but not in invasive carcinomas or metastases. In contrast, laminin β1 chain (a constituent of laminin-8) was typically found in vessel walls of carcinomas and their metastases but not in those of normal breast. The expression of laminin-8 increased in a progression-dependent manner. A similar change was observed from laminin-11 (α5β2γ1) to laminin-10 (α5β1γ1) during breast tumor progression. Additionally, laminin-2 (α2β1γ1) appeared in vascular basement membranes of invasive carcinomas and metastases. Chains of laminin-5 (α3β3γ2) were expressed in the ductal epithelium basement membranes of the breast and diminished with tumor progression. Conclusion These results suggest that laminin-2, laminin-8, and laminin-10 are important components of tumor microvessels and may associate with breast

Zebrafish collagen XII is present in embryonic connective tissue sheaths (fascia) and basement membranes.

PubMed

Bader, Hannah L; Keene, Douglas R; Charvet, Benjamin; Veit, Guido; Driever, Wolfgang; Koch, Manuel; Ruggiero, Florence

2009-01-01

Connective tissues ensure the cohesion of the tissues of the body, but also form specialized structures such as tendon and bone. Collagen XII may enhance the stability of connective tissues by bridging collagen fibrils, but its function is still unclear. Here, we used the zebrafish model to visualize its expression pattern in the whole organism. The zebrafish col12a1 gene is homologous to the small isoform of the tetrapod col12a1 gene. In agreement with the biochemical data reported for the small isoform, the zebrafish collagen XII alpha1 chain was characterized as a collagenase sensitive band migrating at approximately 200 kDa. Using newly generated polyclonal antibodies and anti-sense probes, we performed a comprehensive analysis of its expression in developing zebrafish. Collagen XII exhibited a much broader expression pattern than previously thought: it was ubiquitously expressed in the connective tissue sheaths (fascia) that encase the tissues and organs of the body. For example, it was found in sclera, meninges, epimysia and horizontal and vertical myosepta. Collagen XII was also detected in head mesenchyme, pharyngeal arches and within the spinal cord, where it was first expressed within and then at the lateral borders of the floor plate and at the dorsal midline. Furthermore, double immunofluorescence staining with laminin and immunogold electron microscopy revealed that collagen XII is associated with basement membranes. These data suggest that collagen XII is implicated in tissue cohesion by stabilizing fascia and by linking fascia to basement membranes.

Regional differences in the morphological and functional effects of aging on cerebral basement membranes and perivascular drainage of amyloid-β from the mouse brain.

PubMed

Hawkes, Cheryl A; Gatherer, Maureen; Sharp, Matthew M; Dorr, Adrienne; Yuen, Ho Ming; Kalaria, Rajesh; Weller, Roy O; Carare, Roxana O

2013-04-01

Development of cerebral amyloid angiopathy (CAA) and Alzheimer's disease (AD) is associated with failure of elimination of amyloid-β (Aβ) from the brain along perivascular basement membranes that form the pathways for drainage of interstitial fluid and solutes from the brain. In transgenic APP mouse models of AD, the severity of cerebral amyloid angiopathy is greater in the cerebral cortex and hippocampus, intermediate in the thalamus, and least in the striatum. In this study we test the hypothesis that age-related regional variation in (1) vascular basement membranes and (2) perivascular drainage of Aβ contribute to the different regional patterns of CAA in the mouse brain. Quantitative electron microscopy of the brains of 2-, 7-, and 23-month-old mice revealed significant age-related thickening of capillary basement membranes in cerebral cortex, hippocampus, and thalamus, but not in the striatum. Results from Western blotting and immunocytochemistry experiments showed a significant reduction in collagen IV in the cortex and hippocampus with age and a reduction in laminin and nidogen 2 in the cortex and striatum. Injection of soluble Aβ into the hippocampus or thalamus showed an age-related reduction in perivascular drainage from the hippocampus but not from the thalamus. The results of the study suggest that changes in vascular basement membranes and perivascular drainage with age differ between brain regions, in the mouse, in a manner that may help to explain the differential deposition of Aβ in the brain in AD and may facilitate development of improved therapeutic strategies to remove Aβ from the brain in AD. © 2013 The Authors Aging Cell © 2013 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland.

Magnetic basement and crustal structure in the Arabia-Eurasia collision zone from a combined gravity and magnetic model

NASA Astrophysics Data System (ADS)

Mousavi, Naeim; Ebbing, Jörg

2017-04-01

In this study, we investigate the magnetic basement and crustal structure in the region of Iran by inverse and forward modeling of aeromagnetic data and gravity data. The main focus is on the definition of the magnetic top basement. The combination of multiple shallow magnetic sources and an assumed shallow Curie isotherm depth beneath the Iranian Plateau creates a complex magnetic architecture over the area. Qualitative analysis, including pseudo gravity, wavelength filtering and upward continuation allowed a first separation of probable deep and shallow features, like the Sanandaj Sirjan zone, Urumieh Dokhtar Magmatic Assemblage, Kopet Dagh structural unit and Central Iran domain. In the second step, we apply inverse modeling to generate an estimate of the top basement geometry. The initial model was established from top basement to (a) constant depth of 25 km and (b) Moho depth. The inversion result was used as starting model for more detailed modelling in 3D to evaluate the effect of susceptibility heterogeneities in the crust. Subsequently, the model was modified with respect to tectonic and geological characterization of the region. Further modification of model in regards more details of susceptibility distribution was led to separating upper crust to different magnetic domains. In addition, we refined the top basement geometry by using terrestrial gravity observation as well. The best fitting model is consistent with the Curie isotherm depth as the base of magnetization. The Curie isotherm was derived from independent geophysical-petrological model.

In vivo laser confocal microscopy findings in patients with map-dot-fingerprint (epithelial basement membrane) dystrophy

PubMed Central

Kobayashi, Akira; Yokogawa, Hideaki; Sugiyama, Kazuhisa

2012-01-01

Background: The purpose of this study was to investigate pathological changes of the corneal cell layer in patients with map-dot-fingerprint (epithelial basement membrane) dystrophy by in vivo laser corneal confocal microscopy. Methods: Two patients were evaluated using a cornea-specific in vivo laser scanning confocal microscope (Heidelberg Retina Tomograph 2 Rostock Cornea Module, HRT 2-RCM). The affected corneal areas of both patients were examined. Image analysis was performed to identify corneal epithelial and stromal deposits correlated with this dystrophy. Results: Variously shaped (linear, multilaminar, curvilinear, ring-shape, geographic) highly reflective materials were observed in the “map” area, mainly in the basal epithelial cell layer. In “fingerprint” lesions, multiple linear and curvilinear hyporeflective lines were observed. Additionally, in the affected corneas, infiltration of possible Langerhans cells and other inflammatory cells was observed as highly reflective Langerhans cell-like or dot images. Finally, needle-shaped materials were observed in one patient. Conclusion: HRT 2-RCM laser confocal microscopy is capable of identifying corneal microstructural changes related to map-dot-fingerprint corneal dystrophy in vivo. The technique may be useful in elucidating the pathogenesis and natural course of map-dot-fingerprint corneal dystrophy and other similar basement membrane abnormalities. PMID:22888214

Frictional Behavior of Altered Basement Approaching the Nankai Trough

NASA Astrophysics Data System (ADS)

Saffer, D. M.; Ikari, M.; Rooney, T. O.; Marone, C.

2017-12-01

The frictional behavior of basement rocks plays an important role in subduction zone faulting and seismicity. This includes earthquakes seaward of the trench, large megathrust earthquakes where seamounts are subducting, or where the plate interface steps down to basement. In exhumed subduction zone rocks such as the Shimanto complex in Japan, slivers of basalt are entrained in mélange which is evidence of basement involvement in the fault system. Scientific drilling during the Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) recovered basement rock from two reference sites (C0011 and C0012) located seaward of the trench offshore the Kii Peninsula during Integrated Ocean Discovery Program (IODP) Expeditions 322 and 333. The basement rocks are pillow basalts that appear to be heterogeneously altered, resulting in contrasting dense blue material and more vesicular gray material. Major element geochemistry shows differences in silica, calcium oxides and loss-on-ignition between the two types of samples. Minor element geochemistry reveals significant differences in vanadium, chromium, and barium. X-ray diffraction on a bulk sample powder representing an average composition shows a phyllosilicate content of 20%, most of which is expandable clays. We performed laboratory friction experiments in a biaxial testing apparatus as either intact sample blocks, or as gouge powders. We combine these experiments with measurements of Pennsylvania slate for comparison, including a mixed-lithology intact block experiment. Intact Nankai basement blocks exhibit a coefficient of sliding friction of 0.73; for Nankai basement powder, slate powder, slate blocks and slate-on-basement blocks the coefficient of sliding friction ranges from 0.44 to 0.57. At slip rates ranging from 3x10-8 to 3x10-4 m/s we observe predominantly velocity-strengthening frictional behavior, indicating a tendency for stable slip. At rates of < 1x10-6 m/s some velocity-weakening was observed, specifically in

Quantitative Proteome Analysis Reveals Increased Content of Basement Membrane Proteins in Arteries From Patients With Type 2 Diabetes Mellitus and Lower Levels Among Metformin Users.

PubMed

Preil, Simone A R; Kristensen, Lars P; Beck, Hans C; Jensen, Pia S; Nielsen, Patricia S; Steiniche, Torben; Bjørling-Poulsen, Marina; Larsen, Martin R; Hansen, Maria L; Rasmussen, Lars M

2015-10-01

The increased risk of cardiovascular diseases in type 2 diabetes mellitus has been extensively documented, but the origins of the association remain largely unknown. We sought to determine changes in protein expressions in arterial tissue from patients with type 2 diabetes mellitus and moreover hypothesized that metformin intake influences the protein composition. We analyzed nonatherosclerotic repair arteries gathered at coronary bypass operations from 30 patients with type 2 diabetes mellitus and from 30 age- and sex-matched nondiabetic individuals. Quantitative proteome analysis was performed by isobaric tag for relative and absolute quantitation-labeling and liquid chromatography-mass spectrometry, tandem mass spectrometry analysis on individual arterial samples. The amounts of the basement membrane components, α1-type IV collagen and α2-type IV collagen, γ1-laminin and β2-laminin, were significantly increased in patients with diabetes mellitus. Moreover, the expressions of basement membrane components and other vascular proteins were significantly lower among metformin users when compared with nonusers. Patients treated with or without metformin had similar levels of hemoglobin A1c, cholesterol, and blood pressure. In addition, quantitative histomorphometry showed increased area fractions of collagen-stainable material in tunica intima and media among patients with diabetes mellitus. The distinct accumulation of arterial basement membrane proteins in type 2 diabetes mellitus discloses a similarity between the diabetic macroangiopathy and microangiopathy and suggests a molecular explanation behind the alterations in vascular remodeling, biomechanical properties, and aneurysm formation described in diabetes mellitus. The lower amounts of basement membrane components in metformin-treated individuals are compatible with the hypothesis of direct beneficial drug effects on the matrix composition in the vasculature. © 2015 American Heart Association, Inc.

Fluid Mechanics of the Vascular Basement Membrane in the Brain

NASA Astrophysics Data System (ADS)

Coloma, Mikhail; Hui, Jonathan; Chiarot, Paul; Huang, Peter; Carare, Roxana; McLeod, Kenneth; Schaffer, David

2013-11-01

Beta-amyloid is a normal product of brain metabolic function and is found within the interstitial fluid of the brain. Failure of the clearance of beta-amyloid from the aging brain leads to its accumulation within the walls of arteries and to Alzheimer's disease. The vascular basement membrane (VBM) within the walls of cerebral arteries surrounds the spirally arranged smooth muscle cells and represents an essential pathway for removal of beta-amyloid from the brain. This process fails with the stiffening of arterial walls associated with aging. In this study we hypothesize that the deformation of the VBM associated with arterial pulsations drives the interstitial fluid to drain in the direction opposite of the arterial blood flow. This hypothesis is theoretically investigated by modeling the VBM as a thin, coaxial, fluid-filled porous medium surrounding a periodically deforming cylindrical tube. Flow and boundary conditions required to achieve such a backward clearance are derived through a control volume analysis of mass, momentum, and energy.

Soy protein preserves basement membrane integrity through a synergistic effect on nephrin, matrix metalloproteinase and vascular endothelial growth factor.

PubMed

Palanisamy, Nallasamy; Anuradha, Carani Venkataraman

2011-01-01

Soy protein improves renal function and prevents albuminuria in diabetic rats. This study investigates whether the renoprotective effect of soy protein is related to sustenance of basement membrane integrity. Adult male albino rats were randomized into four groups and fed one of the following semi-synthetic diets consisting of corn starch (60%) and casein (20%; CCD), fructose (60%) and casein (20%; FCD), fructose (60%) and soy protein (20%; FSD), or corn starch (60%) and soy protein (20%; CSD). Plasma chemistry and renal changes were analyzed after 60 days. FCD rats displayed metabolic derangements and renal ultrastructural changes. FSD rats showed reduction in type IV collagen, tissue inhibitor for matrix metallo-proteinase-2, vascular endothelial growth factor and tumor necrosis factor-α expression and improved matrix metallo-proteinase expression. Renal architecture was preserved in these rats. Soy protein supplementation not only improved insulin sensitivity but also markedly attenuated renal basement membrane changes in fructose diet-fed rats. These findings provide evidence in support of the use of dietary soy protein in patients with diabetic kidney disease. Copyright © 2011 S. Karger AG, Basel.

Alport syndrome and thin glomerular basement membrane nephropathy: a practical approach to diagnosis.

PubMed

Haas, Mark

2009-02-01

Alport syndrome and thin glomerular basement membrane nephropathy (TBMN) are genetically heterogeneous conditions characterized by structural abnormalities in the glomerular basement membrane and an initial presentation that usually involves hematuria. Approximately 40% of patients with TBMN are heterozygous carriers for autosomal recessive Alport syndrome, with mutations at the genetic locus encoding type IV collagen alpha(3) [alpha(3)(IV)] and alpha(4) chains. However, although the clinical course of TBMN is usually benign, Alport syndrome, particularly the X-linked form with mutations in the locus encoding the alpha(5) chain of type IV collagen [alpha(5)(IV)], typically results in end-stage renal disease. Electron microscopy is essential to diagnosis of TBMN and Alport syndrome on renal biopsy, although electron microscopy alone is of limited value in distinguishing between TBMN, the heterozygous carrier state of X-linked Alport syndrome, autosomal recessive Alport syndrome, and even early stages of X-linked Alport syndrome. To review diagnostic pathologic features of each of the above conditions, emphasizing the need for immunohistology for alpha(3)(IV) and alpha(5)(IV) in addition to electron microscopy to resolve this differential diagnosis on a renal biopsy. The diagnostic value of immunofluorescence studies for alpha(5)(IV) on a skin biopsy in family members of patients with Alport syndrome also is reviewed. Original and comprehensive review articles on the diagnosis of Alport syndrome and TBMN from the past 35 years, primarily the past 2 decades, and experience in our own renal pathology laboratory. Although Alport syndrome variants and TBMN do not show characteristic light microscopic findings and can be difficult to differentiate from each other even by electron microscopy, using a combination of electron microscopy and immunohistology for alpha(3)(IV) and alpha(5)(IV) enables pathologists to definitively diagnose these disorders on renal biopsy in most

Fractone-heparan sulphates mediate FGF-2 stimulation of cell proliferation in the adult subventricular zone.

PubMed

Douet, V; Kerever, A; Arikawa-Hirasawa, E; Mercier, F

2013-04-01

Fractones are extracellular matrix structures that form a niche for neural stem cells and their immediate progeny in the subventricular zone of the lateral ventricle (SVZa), the primary neurogenic zone in the adult brain. We have previously shown that heparan sulphates (HS) associated with fractones bind fibroblast growth factor-2 (FGF-2), a powerful mitotic growth factor in the SVZa. Here, our objective was to determine whether the binding of FGF-2 to fractone-HS is implicated in the mechanism leading to cell proliferation in the SVZa. Heparitinase-1 was intracerebroventricularly injected with FGF-2 to N-desulfate HS proteoglycans and determine whether the loss of HS and of FGF-2 binding to fractones modifies FGF-2 effect on cell proliferation. We also examined in vivo the binding of Alexa-Fluor-FGF-2 in relationship with the location of HS immunoreactivity in the SVZa. Heparatinase-1 drastically reduced the stimulatory effect of FGF-2 on cell proliferation in the SVZa. Alexa-Fluor-FGF-2 binding was strictly co-localized with HS immunoreactivity in fractones and adjacent vascular basement membranes in the SVZa. Our results demonstrate that FGF-2 requires HS to stimulate cell proliferation in the SVZa and suggest that HS associated with fractones and vascular basement membranes are responsible for activating FGF-2. Therefore, fractones and vascular basement membranes may function as a HS niche to drive cell proliferation in the adult neurogenic zone. © 2013 Blackwell Publishing Ltd.

A Novel Human Adipocyte-derived Basement Membrane for Tissue Engineering Applications

NASA Astrophysics Data System (ADS)

Damm, Aaron

Tissue engineering strategies have traditionally focused on the use of synthetic polymers as support scaffolds for cell growth. Recently, strategies have shifted towards a natural biologically derived scaffold, with the main focus on decellularized organs. Here, we report the development and engineering of a scaffold naturally secreted by human preadipocytes during differentiation. During this differentiation process, the preadipocytes remodel the extracellular matrix by releasing new extracellular proteins. Finally, we investigated the viability of the new basement membrane as a scaffold for tissue engineering using human pancreatic islets, and as a scaffold for soft tissue repair. After identifying the original scaffold material, we sought to improve the yield of material, treating the cell as a bioreactor, through various nutritional and cytokine stimuli. The results suggest that adipocytes can be used as bioreactors to produce a designer-specified engineered human extracellular matrix scaffold for specific tissue engineering applications.

Contribution of alpha3(IV)alpha4(IV)alpha5(IV) Collagen IV to the Mechanical Properties of the Glomerular Basement Membrane

NASA Astrophysics Data System (ADS)

Gyoneva, Lazarina

The glomerular basement membrane (GBM) is a vital part of the blood-urine filtration barrier in the kidneys. In healthy GBMs, the main tension-resisting component is alpha3(IV)alpha4(IV)alpha5(IV) type IV collagen, but in some diseases it is replaced by other collagen IV isoforms. As a result, the GBM becomes leaky and disorganized, ultimately resulting in kidney failure. Our goal is to understanding the biomechanical aspects of the alpha3(IV)alpha4(IV)alpha5(IV) chains and how their absence could be responsible for (1) the initial injury to the GBM and (2) progression to kidney failure. A combination of experiments and computational models were designed for that purpose. A model basement membrane was used to compare experimentally the distensibility of tissues with the alpha3(IV)alpha4(IV)alpha5(IV) chains present and missing. The experiments showed basement membranes containing alpha3(IV)alpha4(IV)alpha5(IV) chains were less distensible. It has been postulated that the higher level of lateral cross-linking (supercoiling) in the alpha3(IV)alpha4(IV)alpha5(IV) networks contributes additional strength/stability to basement membranes. In a computational model of supercoiled networks, we found that supercoiling greatly increased the stiffness of collagen IV networks but only minimally decreased the permeability, which is well suited for the needs of the GBM. It is also known that the alpha3(IV)alpha4(IV)alpha5(IV) networks are more protected from enzymatic degradation, and we explored their significance in GBM remodeling. Our simulations showed that the more protected network was needed to prevent the system from entering a dangerous feedback cycle due to autoregulation mechanisms in the kidneys. Overall, the work adds to the evidence of biomechanical differences between the alpha3(IV)alpha4(IV)alpha5(IV) networks and other collagen IV networks, points to supercoiling as the main source of biomechanical differences, discusses the suitability of alpha3(IV)alpha4(IV

Temporal retinal thinning and the diagnosis of Alport syndrome and Thin basement membrane nephropathy.

PubMed

Chen, Yan; Colville, Deb; Ierino, Francesco; Symons, Andrew; Savige, Judy

2018-04-01

Alport syndrome is an inherited disease characterized by renal failure, hearing loss, and ocular abnormalities, including temporal retinal thinning. This study compared retinal thinning in Alport syndrome and other renal diseases. Alport syndrome was diagnosed on renal biopsy and genetic testing. Subjects underwent optical coherence tomography (OCT) (Spectralis OCT, Heidelberg Instruments). Retinal thinning was determined from horizontal macular OCT scans through the foveal center using the formula: Temporal thickness index (TTI) = (nasal - temporal thickness) ÷ nasal thickness × 100%, and compared with the normal range for each age group. Statistical analysis was performed using Student's t test, Mann-Whitney U test, and ROC analysis (SPPS, IBM). The mean temporal retinal thickness index was 12.4 ± 5.2% in men (n = 19) and 7.4 ± 1.4% in women (n = 28) with X-linked Alport syndrome; 13.1 ± 4.5% (n = 4) in recessive disease; 6.4 ± 2.2% (n = 5) in Thin basement membrane nephropathy; and 6.3 ± 3.3% (n = 14) in other renal diseases. Thinning was worse in men than women with X-linked disease (p < 0.01), and worse in men who developed early onset renal failure (R 2  = 0.75). Temporal retinal thinning was 84% sensitive for men with X-linked Alport syndrome and 67% specific (AUC = 0.83) compared with other renal diseases. Retinal temporal thinning is diagnostic for X-linked Alport syndrome in men and distinguishes them this condition from Thin basement membrane nephropathy, but only in men (p = 0.002). Temporal retinal thinning may also identify men and women with the rarer autosomal recessive disease.

Kidney diseases caused by glomerular basement membrane type IV collagen defects in dogs.

PubMed

Lees, George E

2013-01-01

To review the pathogenesis, as well as the clinical and pathologic features of canine glomerular diseases caused by genetic type IV collagen defects. Original studies and review articles from human and veterinary medical fields. Presence in glomerular basement membranes (GBM) of a network composed of α3.α4.α5 heterotrimers of type IV collagen is required to maintain structure and function of glomerular capillary walls. Hereditary nephropathy (HN) is the most commonly used name for kidney diseases that occur in dogs due to genetic type IV collagen abnormalities. To date, 4 different collagen IV gene mutations have been identified in dogs with HN; 2 are COL4A5 mutations that cause X-linked HN (XL-HN), and 2 are COL4A4 mutations that cause autosomal recessive HN (AR-HN). Affected males with XL-HN and affected males and females with AR-HN develop juvenile-onset kidney disease manifested by proteinuria typically starting at 3-6 months of age and followed by progressive kidney disease leading to terminal failure usually at 6-24 months of age. Carrier female dogs with XL-HN also develop proteinuria starting at 3-6 months of age, but progressive disease causing kidney failure is uncommon until they are >5 years old. The distinctive pathologic lesions of HN are extensive multilaminar splitting and thickening of the GBM, as demonstrated by electron microscopy, and abnormal type IV collagen α-chain content of basement membranes, as demonstrated by immunolabeling. Identification of the underlying gene mutations has permitted genetic testing and selective breeding practices that currently are minimizing HN in breeds known to be at risk. Canine HN is a rare disease that should be considered whenever a dog exhibits a juvenile-onset kidney disease characterized partly by proteinuria, but highly specialized methods are required to pursue a definitive diagnosis. © Veterinary Emergency and Critical Care Society 2013.

Membrane Contact Sites: Complex Zones for Membrane Association and Lipid Exchange

PubMed Central

Quon, Evan; Beh, Christopher T.

2015-01-01

Lipid transport between membranes within cells involves vesicle and protein carriers, but as agents of nonvesicular lipid transfer, the role of membrane contact sites has received increasing attention. As zones for lipid metabolism and exchange, various membrane contact sites mediate direct associations between different organelles. In particular, membrane contact sites linking the plasma membrane (PM) and the endoplasmic reticulum (ER) represent important regulators of lipid and ion transfer. In yeast, cortical ER is stapled to the PM through membrane-tethering proteins, which establish a direct connection between the membranes. In this review, we consider passive and facilitated models for lipid transfer at PM–ER contact sites. Besides the tethering proteins, we examine the roles of an additional repertoire of lipid and protein regulators that prime and propagate PM–ER membrane association. We conclude that instead of being simple mediators of membrane association, regulatory components of membrane contact sites have complex and multilayered functions. PMID:26949334

Proteolytic processing of lysyl oxidase-like-2 in the extracellular matrix is required for crosslinking of basement membrane collagen IV.

PubMed

López-Jiménez, Alberto J; Basak, Trayambak; Vanacore, Roberto M

2017-10-13

Lysyl oxidase-like-2 (LOXL2) is an enzyme secreted into the extracellular matrix that crosslinks collagens by mediating oxidative deamination of lysine residues. Our previous work demonstrated that this enzyme crosslinks the 7S domain, a structural domain that stabilizes collagen IV scaffolds in the basement membrane. Despite its relevant role in extracellular matrix biosynthesis, little is known about the structural requirements of LOXL2 that enable collagen IV crosslinking. In this study, we demonstrate that LOXL2 is processed extracellularly by serine proteases, generating a 65-kDa form lacking the first two scavenger receptor cysteine-rich domains. Site-specific mutagenesis to prevent proteolytic processing generated a full-length enzyme that is active in vitro toward a soluble substrate, but fails to crosslink insoluble collagen IV within the extracellular matrix. In contrast, the processed form of LOXL2 binds to collagen IV and crosslinks the 7S domain. Together, our data demonstrate that proteolytic processing is an important event that allows LOXL2-mediated crosslinking of basement membrane collagen IV. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

VP08R from Infectious Spleen and Kidney Necrosis Virus Is a Novel Component of the Virus-Mock Basement Membrane

PubMed Central

Xu, Xiaopeng; Yan, Muting; Wang, Rui; Lin, Ting; Tang, Junliang; Li, Chaozheng; Weng, Shaoping

2014-01-01

ABSTRACT Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus Megalocytivirus, family Iridoviridae, brings great harm to fish farming. In infected tissues, ISKNV infection is characterized by a unique phenomenon, in that the infected cells are attached by lymphatic endothelial cells (LECs), which are speculated to wall off the infected cells from host immune attack. A viral membrane protein, VP23R, binds and recruits the host nidogen-1 protein to construct a basement membrane (BM)-like structure, termed virus-mock basement membrane (VMBM), on the surface of infected cells to provide attaching sites for LECs. VMBMs do not contain collagen IV protein, which is essential for maintenance of BM integrity and functions. In this study, we identified the VP08R protein encoded by ISKNV. VP08R was predicted to be a secreted protein with a signal peptide but without a transmembrane domain. However, immunofluorescence assays demonstrated that VP08R is located on the plasma membrane of infected cells and shows an expression profile similar to that of VP23R. Coimmunoprecipitation showed that VP08R interacts with both VP23R and nidogen-1, indicating that VP08R is a component of VMBM and is present on the cell membrane by binding to VP23R. Through formation of intermolecular disulfide bonds, VP08R molecules self-organized into a multimer, which may play a role in the maintenance of VMBM integrity and stability. Moreover, the VP08R multimer was easily degraded when the ISKNV-infected cells were lysed, which may be a mechanism for VMBM disassembly when necessary to free LECs and release the mature virions. IMPORTANCE Infectious spleen and kidney necrosis virus (ISKNV; genus Megalocytivirus, family Iridovirus) is most harmful to cultured fishes. In tissues, the ISKNV-infected cells are attached by lymphatic endothelial cells (LECs), which are speculated to segregate the host immune system. A viral membrane protein, VP23R, binds and recruits the host

Mast cells distribution and variations in epithelium thickness and basement membrane in oral lichen planus lesion and oral lichenoid reaction

PubMed Central

Jahanshahi, Gholamreza; Ghalayani, Parichehr; Maleki, Laleh

2012-01-01

Background: Oral lichen planus (OLP) is a chronic mucocutaneous lesion with unknown etiology. Oral lichenoid lesions (OLL) comprise a family of lesions with different etiologies. Both lesions have similar clinical and histopathologic characteristics although their management is different. Differential diagnosis between OLP and OLL has always been a major challenge. Materials and Methods: In this prospective analytical study, the role of mast cells in pathogenesis of these lesions was investigated by evaluation of 52 patients with clinical and histopathological diagnosis of OLP (26 cases) and OLL (26 cases) based on WHO criteria, and by applying a more accessible staining methods, Hematoxylin and Eosin, toluidine blue (histochemistry) and Periodic Acid Schiff staining. In order to distinguish these two lesions, number of mast cells and thickness of epithelium and basement membrane were measured using light microscopy. Data were analyzed by SPSS software using t-test method (P<0.001). Results: No significant difference was observed between the total numbers of mast cells of two groups (P=0.148), but a statistically significant difference was detected between degranulated mast cells in two groups (P<0.001). A significant difference was also observed between the thickness of epithelium in two groups (P<0.001), although no difference was seen between basement membrane thickness in these lesions. Conclusion: Number of degranulated mast cells in reticular layer of corium in lichenoid lesions was more than that of OLP. This implies that despite the increase in number of these cells, in both groups of diseases, the role of these cells has not been the same in pathogenesis of the diseases. Moreover, the epithelium thickness was lower in lesions of OLP compared to lesions of oral lichenoid, so this parameter may be a useful criterion together with other histopathological and clinical finding to discriminate these lesions. However, discrepancy of basement membrane thickness

Age-related morphological changes in the basement membrane in the stria vascularis of C57BL/6 mice.

PubMed

Suzuki, Mitsuya; Sakamoto, Takashi; Kashio, Akinori; Yamasoba, Tatsuya

2016-01-01

Basement membrane anionic sites (BMAS) are involved in the selective transport of electrically charged macromolecules in cochlear capillaries. Using cationic polyethyleneimine (PEI), we examined age-related changes in BMAS in the cochleae of C57BL/6 mice. The mice were grouped according to age as follows: 3 days, 4 weeks, 8 weeks, 6 months, and 12 months. In the right bony labyrinths, widths of the stria vascularis were measured in paraffin-embedded sections using light microscopy. The left bony labyrinths were immersed in a 0.5 % cationic PEI solution and embedded in epoxy resin. Ultrathin sections of the left cochlea were examined using transmission electron microscopy. A significant difference in stria vascularis width was observed between the 4-week-old and 12-month-old mice. The PEI distribution in the capillary and epithelial basement membranes (BMs) of the cochlea was observed. In all animals, PEI particles were evenly distributed in the capillary BM of the spiral ligament and in the subepithelial BM of Reissner's membrane. In the stria vascularis, PEI particles were evenly distributed in the capillary BM in 3-day-old mice. In 4- and 8-week-old mice, PEI particle sizes were markedly lower than those observed in 3-day-old mice. In 6- and 12-month-old mice, PEI particles were hardly detected in the strial capillary BM. In the strial capillary BM in these mice, the laminae rarae externa and interna disappeared, but the lamina densa became larger. We speculated that age-related changes of strial capillary BMAS may affect electrically charged macromolecule transport systems in the stria vascularis of C57BL/6 mice.

Podocytes regulate the glomerular basement membrane protein nephronectin by means of miR-378a-3p in glomerular diseases.

PubMed

Müller-Deile, Janina; Dannenberg, Jan; Schroder, Patricia; Lin, Meei-Hua; Miner, Jeffrey H; Chen, Rongjun; Bräsen, Jan-Hinrich; Thum, Thomas; Nyström, Jenny; Staggs, Lynne Beverly; Haller, Hermann; Fiedler, Jan; Lorenzen, Johan M; Schiffer, Mario

2017-10-01

The pathophysiology of many proteinuric kidney diseases is poorly understood, and microRNAs (miRs) regulation of these diseases has been largely unexplored. Here, we tested whether miR-378a-3p is a novel regulator of glomerular diseases. MiR-378a-3p has two predicted targets relevant to glomerular function, the glomerular basement membrane matrix component, nephronectin (NPNT), and vascular endothelial growth factor VEGF-A. In zebrafish (Danio rerio), miR-378a-3p mimic injection or npnt knockdown by a morpholino oligomer caused an identical phenotype consisting of edema, proteinuria, podocyte effacement, and widening of the glomerular basement membrane in the lamina rara interna. Zebrafish vegf-A protein could not rescue this phenotype. However, mouse Npnt constructs containing a mutated 3'UTR region prevented the phenotype caused by miR-378a-3p mimic injection. Overexpression of miR-378a-3p in mice confirmed glomerular dysfunction in a mammalian model. Biopsies from patients with focal segmental glomerulosclerosis and membranous nephropathy had increased miR-378a-3p expression and reduced glomerular levels of NPNT. Thus, miR-378a-3p-mediated suppression of the glomerular matrix protein NPNT is a novel mechanism for proteinuria development in active glomerular diseases. Copyright © 2017 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.

Endothelial basement membrane limits tip cell formation by inducing Dll4/Notch signalling in vivo.

PubMed

Stenzel, Denise; Franco, Claudio A; Estrach, Soline; Mettouchi, Amel; Sauvaget, Dominique; Rosewell, Ian; Schertel, Andreas; Armer, Hannah; Domogatskaya, Anna; Rodin, Sergey; Tryggvason, Karl; Collinson, Lucy; Sorokin, Lydia; Gerhardt, Holger

2011-10-28

How individual components of the vascular basement membrane influence endothelial cell behaviour remains unclear. Here we show that laminin α4 (Lama4) regulates tip cell numbers and vascular density by inducing endothelial Dll4/Notch signalling in vivo. Lama4 deficiency leads to reduced Dll4 expression, excessive filopodia and tip cell formation in the mouse retina, phenocopying the effects of Dll4/Notch inhibition. Lama4-mediated Dll4 expression requires a combination of integrins in vitro and integrin β1 in vivo. We conclude that appropriate laminin/integrin-induced signalling is necessary to induce physiologically functional levels of Dll4 expression and regulate branching frequency during sprouting angiogenesis in vivo.

Endothelial basement membrane limits tip cell formation by inducing Dll4/Notch signalling in vivo

PubMed Central

Stenzel, Denise; Franco, Claudio A; Estrach, Soline; Mettouchi, Amel; Sauvaget, Dominique; Rosewell, Ian; Schertel, Andreas; Armer, Hannah; Domogatskaya, Anna; Rodin, Sergey; Tryggvason, Karl; Collinson, Lucy; Sorokin, Lydia; Gerhardt, Holger

2011-01-01

How individual components of the vascular basement membrane influence endothelial cell behaviour remains unclear. Here we show that laminin α4 (Lama4) regulates tip cell numbers and vascular density by inducing endothelial Dll4/Notch signalling in vivo. Lama4 deficiency leads to reduced Dll4 expression, excessive filopodia and tip cell formation in the mouse retina, phenocopying the effects of Dll4/Notch inhibition. Lama4-mediated Dll4 expression requires a combination of integrins in vitro and integrin β1 in vivo. We conclude that appropriate laminin/integrin-induced signalling is necessary to induce physiologically functional levels of Dll4 expression and regulate branching frequency during sprouting angiogenesis in vivo. PMID:21979816

Precambrian crystalline basement map of Idaho-an interpretation of aeromagnetic anomalies

USGS Publications Warehouse

Sims, P.K.; Lund, Karen; Anderson, E.

2005-01-01

Idaho lies within the northern sector of the U.S. Cordillera astride the boundary between the Proterozoic continent (Laurentia) to the east and the Permian to Jurassic accreted terranes to the west. The continental basement is mostly covered by relatively undeformed Mesoproterozoic metasedimentary rocks and intruded or covered by Phanerozoic igneous rocks; accordingly, knowledge of the basement geology is poorly constrained. Incremental knowledge gained since the pioneering studies by W. Lindgren, C.P. Ross, A.L. Anderson, A. Hietanen, and others during the early- and mid-1900's has greatly advanced our understanding of the general geology of Idaho. However, knowledge of the basement geology remains relatively poor, partly because of the remoteness of much of the region plus the lack of a stimulus to decipher the complex assemblage of high-grade gneisses and migmatite of central Idaho. The availability of an updated aeromagnetic anomaly map of Idaho (North American Magnetic Anomaly Group, 2002) provides a means to determine the regional Precambrian geologic framework of the State. The combined geologic and aeromagnetic data permit identification of previously unrecognized crystalline basement terranes, assigned to Archean and Paleoproterozoic ages, and the delineation of major shear zones, which are expressed in the aeromagnetic data as linear negative anomalies (Finn and Sims, 2004). Limited geochronologic data on exposed crystalline basement aided by isotopic studies of zircon inheritance, particularly Bickford and others (1981) and Mueller and others (1995), provide much of the geologic background for our interpretation of the basement geology. In northwestern United States, inhomogeneities in the basement inherited from Precambrian tectogenesis controlled many large-scale tectonic features that developed during the Phanerozoic. Two basement structures, in particular, provided zones of weakness that were repeatedly rejuvenated: (1) northeast-trending ductile

Enamel organic matrix: potential structural role in enamel and relationship to residual basement membrane constituents at the dentin enamel junction

PubMed Central

McGuire, Jacob D.; Walker, Mary P.; Dusevich, Vladimir; Wang, Yong; Gorski, Jeff P.

2015-01-01

Although mature enamel is predominantly composed of mineral, a previously uncharacterized organic matrix layer remains in the post-eruptive tissue that begins at the dentin enamel junction and extends 200–300 µm towards the outer tooth surface. Identification of the composition of this layer has been hampered by its insolubility; however, we have developed a single step method to isolate the organic enamel matrix relatively intact. After dissociative dissolution of the matrix with SDS and urea, initial characterization by Western blotting and gel zymography indicates the presence of type IV and type VII basement membrane collagens and active matrix metalloproteinase-20. When combined with data from transgenic knockout mice and from human mutations, these data suggest that the enamel organic matrix (EOM) and dentin enamel junction may have a structural and functional relationship with basement membranes, e.g. skin. To clarify this relationship, we hypothesize a “foundation” model which proposes that components of the EOM form a support structure that stabilizes the crystalline enamel layer, and bonds it to the underlying dentin along the dentin enamel junction. Since we have also co-localized an active matrix metalloproteinase to this layer, our hypothesis suggests that, under pathologic conditions, MMP-mediated degradation of the EOM could destabilize the enamel–dentin interface. PMID:25158177

Basement-driven strike-slip deformation involving a salt-stock canopy system

NASA Astrophysics Data System (ADS)

Dooley, Tim; Jackson, Martin; Hudec, Mike

2016-04-01

NW-striking basement-involved strike-slip zones have been reported or inferred from the northern Gulf of Mexico (GoM). This interpretation is uncertain, because the effects of strike-slip deformation are commonly difficult to recognize in cross sections. Recognition is doubly difficult if the strike-slip zone passes through a diapir field that complicates deformation, and an associated salt canopy that partially decouples shallow deformation from deep deformation. We use physical models to explore the effects of strike-slip deformation above and below a salt-stock canopy system. Canopies of varying maturity grew from a series of 14 feeders/diapirs located on and off the axis of a dextral basement fault. Strike-slip deformation styles in the overburden vary significantly depending on: (1) the location of the diapirs with respect to the basement fault trace, and; (2) the continuity of the canopy system. On-axis diapirs (where the diapirs lie directly above the basement fault) are typically strongly deformed and pinched shut at depth to form sharp S-shapes, whereas their shallow deformation style is that of a open-S-shaped pop-up structure in a restraining bend. The narrow diapir stem acts as a shear zone at depth. Pull-apart structures form between diapirs that are arranged in a right-stepping array tangental to the basement fault trace. These grade along strike into narrow negative flower structures. Off-axis diapirs (diapirs laterally offset from the basement fault but close enough to participate in the deformation) form zones of distributed deformation in the form of arrays of oblique faults (R shears) that converge along strike onto the narrower deformation zones associated with on-axis diapirs. Above an immature, or patchy, canopy system the strike-slip structures closely match sub canopy structures, with the exception of wrench fold formation where the supracanopy roof is thin. In contrast, the surface structures above a mature canopy system consist of a broad

Interior detail of basement door, woodenlouvered transom and glass doorknob, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Interior detail of basement door, wooden-louvered transom and glass doorknob, facing southeast. - Gorgas Hospital, Administration & Clinics Building, Culebra Road, Balboa Heights, Former Panama Canal Zone, CZ

Fault geometries in basement-induced wrench faulting under different initial stress states

NASA Astrophysics Data System (ADS)

Naylor, M. A.; Mandl, G.; Supesteijn, C. H. K.

Scaled sandbox experiments were used to generate models for relative ages, dip, strike and three-dimensional shape of faults in basement-controlled wrench faulting. The basic fault sequence runs from early en échelon Riedel shears and splay faults through 'lower-angle' shears to P shears. The Riedel shears are concave upwards and define a tulip structure in cross-section. In three dimensions, each Riedel shear has a helicoidal form. The sequence of faults and three-dimensional geometry are rationalized in terms of the prevailing stress field and Coulomb-Mohr theory of shear failure. The stress state in the sedimentary overburden before wrenching begins has a substantial influence on the fault geometries and on the final complexity of the fault zone. With the maximum compressive stress (∂ 1) initially parallel to the basement fault (transtension), Riedel shears are only slightly en échelon, sub-parallel to the basement fault, steeply dipping with a reduced helicoidal aspect. Conversely, with ∂ 1 initially perpendicular to the basement fault (transpression), Riedel shears are strongly oblique to the basement fault strike, have lower dips and an exaggerated helicoidal form; the final fault zone is both wide and complex. We find good agreement between the models and both mechanical theory and natural examples of wrench faulting.

Collagen IV and basement membrane at the evolutionary dawn of metazoan tissues

PubMed Central

Fidler, Aaron L; Darris, Carl E; Chetyrkin, Sergei V; Pedchenko, Vadim K; Boudko, Sergei P; Brown, Kyle L; Gray Jerome, W; Hudson, Julie K; Rokas, Antonis; Hudson, Billy G

2017-01-01

The role of the cellular microenvironment in enabling metazoan tissue genesis remains obscure. Ctenophora has recently emerged as one of the earliest-branching extant animal phyla, providing a unique opportunity to explore the evolutionary role of the cellular microenvironment in tissue genesis. Here, we characterized the extracellular matrix (ECM), with a focus on collagen IV and its variant, spongin short-chain collagens, of non-bilaterian animal phyla. We identified basement membrane (BM) and collagen IV in Ctenophora, and show that the structural and genomic features of collagen IV are homologous to those of non-bilaterian animal phyla and Bilateria. Yet, ctenophore features are more diverse and distinct, expressing up to twenty genes compared to six in vertebrates. Moreover, collagen IV is absent in unicellular sister-groups. Collectively, we conclude that collagen IV and its variant, spongin, are primordial components of the extracellular microenvironment, and as a component of BM, collagen IV enabled the assembly of a fundamental architectural unit for multicellular tissue genesis. DOI: http://dx.doi.org/10.7554/eLife.24176.001 PMID:28418331

Cadherin 11 Involved in Basement Membrane Damage and Dermal Changes in Melasma.

PubMed

Kim, Nan-Hyung; Choi, Soo-Hyun; Lee, Tae Ryong; Lee, Chang-Hoon; Lee, Ai-Young

2016-06-15

Basement membrane (BM) disruption and dermal changes (elastosis, collagenolysis, vascular ectasia) have been reported in melasma. Although ultraviolet (UV) irradiation can induce these changes, UV is not always necessary for melasma development. Cadherin 11 (CDH11), which is upregulated in some melasma patients, has previously been shown to stimulate melanogenesis. Because CDH11 action requires cell-cell adhesion between fibroblasts and melanocytes, BM disruption in vivo should facilitate this. The aim of this study was to examine whether CDH11 overexpression leads to BM disruption and dermal changes, independent of UV irradiation. Immunohistochemistry/immunofluorescence, real-time PCR, Western blotting, and zymography suggested that BM disruption/dermal changes and related factors were present in the hyperpigmented skin of CDH11-upregulated melasma patients and in CDH11-overexpressing fibroblasts/keratinocytes. The opposite was seen in CDH11-knockdown cells. UV irradiation of the cultured cells did not increase CDH11 expression. Collectively, these data demonstrate that CDH11 overexpression could induce BM disruption and dermal changes in melasma, regardless of UV exposure.

Regional trends in radiogenic heat generation in the Precambrian basement of the Western Canadian Basin

NASA Astrophysics Data System (ADS)

Jones, F. W.; Majorowicz, J. A.

Radiogenic heat generation values for 381 basement samples from 229 sites in the western Canadian basin exhibit a lognormal frequency distribution. The mean value = 2.06 (S.D. = 1.22) µWm-3 is larger than the radiogenic heat generation values reported for the shield in the Superior (ca. 1.2 µWm-3, Jessop and Lewis, 1978) and Churchill (ca. 0.7 µWm-3, Drury, 1985) provinces. When equal Log A contour intervals are used to map the basement heat generation, three large zones of relatively high heat generation are found. One coincides with the Peace River Arch basement structure and one with the Athabasca axis (Darnley, 1981). There is no apparent indication of increased heat flow through the Paleozoic formations associated with these two zones. The third zone, in southwestern Saskatchewan, coincides with a high heat flow zone in the Swift Current area. The lack of correlation between heat flow and heat generation in Alberta may be due to the disturbance to the heat flow in the Paleozoic formations by water motion, or may indicate that the heat is from uranium, thorium and potassium isotope enrichment near the basement surface rather than enrichment throughout the entire upper crust.

Feasibility of Repairing Glomerular Basement Membrane Defects in Alport Syndrome

PubMed Central

Lin, Xiaobo; Suh, Jung Hee; Go, Gloriosa

2014-01-01

Alport syndrome is a hereditary glomerular disease that leads to kidney failure. It is caused by mutations affecting one of three chains of the collagen α3α4α5(IV) heterotrimer, which forms the major collagen IV network of the glomerular basement membrane (GBM). In the absence of the α3α4α5(IV) network, the α1α1α2(IV) network substitutes, but it is insufficient to maintain normal kidney function. Inhibition of angiotensin-converting enzyme slows progression to kidney failure in patients with Alport syndrome but is not a cure. Restoration of the normal collagen α3α4α5(IV) network in the GBM, by either cell- or gene-based therapy, is an attractive and logical approach toward a cure, but whether or not the abnormal GBM can be repaired once it has formed and is functioning is unknown. Using a mouse model of Alport syndrome and an inducible transgene system, we found that secretion of α3α4α5(IV) heterotrimers by podocytes into a preformed, abnormal, filtering Alport GBM is effective at restoring the missing collagen IV network, slowing kidney disease progression, and extending life span. This proof-of-principle study demonstrates the plasticity of the mature GBM and validates the pursuit of therapeutic approaches aimed at normalizing the GBM to prolong kidney function. PMID:24262794

Feasibility of repairing glomerular basement membrane defects in Alport syndrome.

PubMed

Lin, Xiaobo; Suh, Jung Hee; Go, Gloriosa; Miner, Jeffrey H

2014-04-01

Alport syndrome is a hereditary glomerular disease that leads to kidney failure. It is caused by mutations affecting one of three chains of the collagen α3α4α5(IV) heterotrimer, which forms the major collagen IV network of the glomerular basement membrane (GBM). In the absence of the α3α4α5(IV) network, the α1α1α2(IV) network substitutes, but it is insufficient to maintain normal kidney function. Inhibition of angiotensin-converting enzyme slows progression to kidney failure in patients with Alport syndrome but is not a cure. Restoration of the normal collagen α3α4α5(IV) network in the GBM, by either cell- or gene-based therapy, is an attractive and logical approach toward a cure, but whether or not the abnormal GBM can be repaired once it has formed and is functioning is unknown. Using a mouse model of Alport syndrome and an inducible transgene system, we found that secretion of α3α4α5(IV) heterotrimers by podocytes into a preformed, abnormal, filtering Alport GBM is effective at restoring the missing collagen IV network, slowing kidney disease progression, and extending life span. This proof-of-principle study demonstrates the plasticity of the mature GBM and validates the pursuit of therapeutic approaches aimed at normalizing the GBM to prolong kidney function.

IgA antibasement membrane nephritis with pulmonary hemorrhage.

PubMed

Border, W A; Baehler, R W; Bhathena, D; Glassock, R J

1979-07-01

Goodpasture's syndrome has characteristically been described as being mediated by IgG antibodies. We have recently seen a 55-year-old man who developed renal failure and hemoptysis; a renal biopsy showed linear deposits of IgA and C3 involving glomerular and tubular basement membrane. Serologic tests for detecting (IgG) antiglomerular basement membrane antibodies were negative. Elution studies of kidney and lung showed the presence of an IgA antibasement membrane antibody only. The patient's serum contained IgA, but not IgG, antibodies reactive with glomerular and tubular basement membrane of normal human kidney and alveolar basement membrane of normal human lung. Attempts to transfer disease with the patient's IgA antibody to a monkey and to Lewis and Brown-Norway rats were unsuccessful. Immunoglobulin A antibasement membrane antibody must be considered in the design of immunoserologic procedures for the diagnosis of Goodpasture's syndrome.

Glucuronylated core 1 glycans are required for precise localization of neuromuscular junctions and normal formation of basement membranes on Drosophila muscles.

PubMed

Itoh, Kazuyoshi; Akimoto, Yoshihiro; Kondo, Shu; Ichimiya, Tomomi; Aoki, Kazuhiro; Tiemeyer, Michael; Nishihara, Shoko

2018-04-15

T antigen (Galβ1-3GalNAcα1-Ser/Thr) is an evolutionary-conserved mucin-type core 1 glycan structure in animals synthesized by core 1 β1,3-galactosyltransferase 1 (C1GalT1). Previous studies showed that T antigen produced by Drosophila C1GalT1 (dC1GalT1) was expressed in various tissues and dC1GalT1 loss in larvae led to various defects, including decreased number of circulating hemocytes, hyper-differentiation of hematopoietic stem cells in lymph glands, malformation of the central nervous system, mislocalization of neuromuscular junction (NMJ) boutons, and ultrastructural abnormalities in NMJs and muscle cells. Although glucuronylated T antigen (GlcAβ1-3Galβ1-3GalNAcα1-Ser/Thr) has been identified in Drosophila, the physiological function of this structure has not yet been clarified. In this study, for the first time, we unraveled biological roles of glucuronylated T antigen. Our data show that in Drosophila, glucuronylation of T antigen is predominantly carried out by Drosophila β1,3-glucuronyltransferase-P (dGlcAT-P). We created dGlcAT-P null mutants and found that mutant larvae showed lower expression of glucuronylated T antigen on the muscles and at NMJs. Furthermore, mislocalization of NMJ boutons and a partial loss of the basement membrane components collagen IV (Col IV) and nidogen (Ndg) at the muscle 6/7 boundary were observed. Those two phenotypes were correlated and identical to previously described phenotypes in dC1GalT1 mutant larvae. In addition, dGlcAT-P null mutants exhibited fewer NMJ branches on muscles 6/7. Moreover, ultrastructural analysis revealed that basement membranes that lacked Col IV and Ndg were significantly deformed. We also found that the loss of dGlcAT-P expression caused ultrastructural defects in NMJ boutons. Finally, we showed a genetic interaction between dGlcAT-P and dC1GalT1. Therefore, these results demonstrate that glucuronylated core 1 glycans synthesized by dGlcAT-P are key modulators of NMJ bouton localization

Timing and conditions of regional metamorphism and crustal shearing in the granulite facies basement of south Namibia: Implications for the crustal evolution of the Namaqualand metamorphic basement in the Mesoproterozoic

NASA Astrophysics Data System (ADS)

Bial, Julia; Büttner, Steffen; Appel, Peter

2016-11-01

Granulite facies basement gneisses from the Grünau area in the Kakamas Domain of the Namaqua-Natal Metamorphic Province in south Namibia show high-grade mineral assemblages, most commonly consisting of garnet, cordierite, sillimanite, alkali feldspar and quartz. Cordierite + hercynitic spinel, and in some places quartz + hercynitic spinel, indicate granulite facies P-T conditions. The peak assemblage equilibrated at 800-850 °C at 4.0-4.5 kbar. Sillimanite pseudomorphs after kyanite1 and late-stage staurolite and kyanite2 indicate that the metamorphic record started and ended within the stability field of kyanite. Monazite in the metamorphic basement gneisses shows a single-phase growth history dated as 1210-1180 Ma, which we interpret as the most likely age of the regional metamorphic peak. This time coincides with the emplacement of granitic plutons in the Grünau region. The ∼10 km wide, NW-SE striking Grünau shear zone crosscuts the metamorphic basement and overprints high-temperature fabrics. In sheared metapelites, the regional metamorphic peak assemblage is largely obliterated, and is replaced by synkinematic biotite2, quartz, alkali feldspar, sillimanite and cordierite or muscovite. In places, gedrite, staurolite, sillimanite and green biotite3 may have formed late- or post-kinematically. The mylonitic mineral assemblage equilibrated at 590-650 °C at 3.5-5.0 kbar, which is similar to a retrograde metamorphic stage in the basement away from the shear zone. Monazite cores in two mylonite samples are similar in texture and age (∼1200 Ma) to monazite in metapelites away from the shear zone. Chemically distinct monazite rims indicate a second growth episode at ∼1130-1120 Ma. This age is interpreted to date the main deformation episode along the Grünau shear zone and the retrograde metamorphic stage seen in the basement. The main episode of ductile shearing along the Grünau shear zone took place 70-80 million years after the thermal peak metamorphism

Basement diapirism associated with the emplacement of major ophiolite nappes: Some constraints

NASA Astrophysics Data System (ADS)

Andrews-Speed, C. P.; Johns, C. C.

1985-09-01

The association of basement uplifts with major ophiolite nappes in some Phanerozoic orogenic belts suggests that gravitational instability results in the local diapiric uplift of the basement following ophiolite emplacement. In previous analyses of diapirism in crustal silicate rocks, viscous behaviour of rocks has been assumed. It is argued that this assumption is not valid. An alternative analysis is offered to determine whether or not the stress would be sufficient for diapirism to occur. The negative buoyancy stress resulting from the emplacement of an ophiolite nappe 5-15 km thick onto continental basement may be in the range 10-50 MPa. If the horizontal deviatoric stress is zero, this will be the maximum principal compressive stress. After ophiolite emplacement the thermal profile through the ophiolite and the basement will relax from a saw-tooth form to an equilibrium profile. If the ophiolite is young and thick there will be a zone of ductile strain in the lower part of the ophiolite and in the upper part of the continental basement. Results from steady-state creep experiments suggest that temperatures in this zone may be high enough for a short time after ophiolite emplacement (3 Ma or more) for the rocks in this zone to deform at geologically significant strain rates (10 -14 or greater) in response to the negative buoyancy stress. A thin ophiolite or rapid erosion will result in this ductile zone being absent or too short-lived for significant strain. Aquaeous fluids may reduce the strength of brittle rocks by decreasing the effective normal stress or by encouraging pressure solution creep. Evidence suggests that the deviatoric stress across presently active faults may be as low at 10 MPa. Thus diapirism in response to ophiolite emplacement may occur through brittle strain. Gravity spreading within the ophiolite is an alternative mechanism for accommodating the gravitational instability. The critical evidence lies in the field.

Effects of plasma proteins on sieving of tracer macromolecules in glomerular basement membrane.

PubMed

Lazzara, M J; Deen, W M

2001-11-01

It was found previously that the sieving coefficients of Ficoll and Ficoll sulfate across isolated glomerular basement membrane (GBM) were greatly elevated when BSA was present at physiological levels, and it was suggested that most of this increase might have been the result of steric interactions between BSA and the tracers (5). To test this hypothesis, we extended the theory for the sieving of macromolecular tracers to account for the presence of a second, abundant solute. Increasing the concentration of an abundant solute is predicted to increase the equilibrium partition coefficient of a tracer in a porous or fibrous membrane, thereby increasing the sieving coefficient. The magnitude of this partitioning effect depends on solute size and membrane structure. The osmotic reduction in filtrate velocity caused by an abundant, mostly retained solute will also tend to elevate the tracer sieving coefficient. The osmotic effect alone explained only about one-third of the observed increase in the sieving coefficients of Ficoll and Ficoll sulfate, whereas the effect of BSA on tracer partitioning was sufficient to account for the remainder. At physiological concentrations, predictions for tracer sieving in the presence of BSA were found to be insensitive to the assumed shape of the protein (sphere or prolate spheroid). For protein mixtures, the theoretical effect of 6 g/dl BSA on the partitioning of spherical tracers was indistinguishable from that of 3 g/dl BSA and 3 g/dl IgG. This suggests that for partitioning and sieving studies in vitro, a good experimental model for plasma is a BSA solution with a mass concentration matching that of total plasma protein. The effect of plasma proteins on tracer partitioning is expected to influence sieving not only in isolated GBM but also in intact glomerular capillaries in vivo.

Ultrastructural study of electron dense deposits in renal tubular basement membrane: prevalence and relationship to epithelial atrophy.

PubMed

Yong, Jim L C; Killingsworth, Murray C

2014-08-01

This study reports the prevalence of immune deposits associated with the proximal and distal tubules in a series of routine renal biopsies received in our department during a single calendar year. From 87 cases, 65 (74%) were found to have glomerular immune deposits by immunofluorescence. Tubular immune deposits were found in 12 cases (18%), 3 of which had no glomerular deposits. By transmission electron microscopy (EM), 58 cases (66%) were found to have deposits of granular or vesicular material associated with the tubular basement membranes (TBM). Finely granular electron dense deposits appeared to correspond to the immune deposits seen by immunofluorescence microscopy (IF) and may be a sensitive marker of immune deposition.

Ultrastructural Study of Electron Dense Deposits in Renal Tubular Basement Membrane: Prevalence and Relationship to Epithelial Atrophy

PubMed Central

Killingsworth, Murray C.

2014-01-01

This study reports the prevalence of immune deposits associated with the proximal and distal tubules in a series of routine renal biopsies received in our department during a single calendar year. From 87 cases, 65 (74%) were found to have glomerular immune deposits by immunofluorescence. Tubular immune deposits were found in 12 cases (18%), 3 of which had no glomerular deposits. By transmission electron microscopy (EM), 58 cases (66%) were found to have deposits of granular or vesicular material associated with the tubular basement membranes (TBM). Finely granular electron dense deposits appeared to correspond to the immune deposits seen by immunofluorescence microscopy (IF) and may be a sensitive marker of immune deposition. PMID:24933115

Metrics of cellular and vascular infiltration of human acellular dermal matrix in ventral hernia repairs.

PubMed

Campbell, Kristin Turza; Burns, Nadja K; Ensor, Joe; Butler, Charles E

2012-04-01

Human acellular dermal matrix is used for ventral hernia repair, as it resists infection and remodels by means of surrounding tissue. However, the tissue source and impact of basement membrane on cell and vessel infiltration have not been determined. The authors hypothesized that musculofascia would be the primary tissue source of cells and vessels infiltrating into human acellular dermal matrix and that the basement membrane would inhibit infiltration. Fifty-six guinea pigs underwent inlay human acellular dermal matrix ventral hernia repair with the basement membrane oriented toward or away from the peritoneum. At postoperative weeks 1, 2, or 4, repair sites were completely excised. Histologic and immunohistochemical analyses were performed to quantify cell and vessel density within repair-site zones, including interface (lateral, beneath musculofascia) and center (beneath subcutaneous fat) zones. Cell and vessel quantities were compared as functions of zone, basement membrane orientation, and time. Cellular and vascular infiltration increased over time universally. The interface demonstrated greater mean cell density than the center (weeks 1 and 2, p = 0.01 and p < 0.0001, respectively). Cell density was greater with the basement membrane oriented toward the peritoneum at week 4 (p = 0.02). The interface zone had greater mean vessel density than the center zone at week 4 (p < 0.0001). Orienting the basement membrane toward the peritoneum increased vessel density at week 4 (p = 0.0004). Cellular and vascular infiltration into human acellular dermal matrix for ventral hernia repairs was greater from musculofascia than from subcutaneous fat, and the basement membrane inhibited cellular and vascular infiltration. Human acellular dermal matrix should be placed adjacent to the best vascularizing tissue to improve fibrovascular incorporation.

Structural decoding of netrin-4 reveals a regulatory function towards mature basement membranes

PubMed Central

Reuten, Raphael; Patel, Trushar R.; McDougall, Matthew; Rama, Nicolas; Nikodemus, Denise; Gibert, Benjamin; Delcros, Jean-Guy; Prein, Carina; Meier, Markus; Metzger, Stéphanie; Zhou, Zhigang; Kaltenberg, Jennifer; McKee, Karen K.; Bald, Tobias; Tüting, Thomas; Zigrino, Paola; Djonov, Valentin; Bloch, Wilhelm; Clausen-Schaumann, Hauke; Poschl, Ernst; Yurchenco, Peter D.; Ehrbar, Martin; Mehlen, Patrick; Stetefeld, Jörg; Koch, Manuel

2016-01-01

Netrins, a family of laminin-related molecules, have been proposed to act as guidance cues either during nervous system development or the establishment of the vascular system. This was clearly demonstrated for netrin-1 via its interaction with the receptors DCC and UNC5s. However, mainly based on shared homologies with netrin-1, netrin-4 was also proposed to play a role in neuronal outgrowth and developmental/pathological angiogenesis via interactions with netrin-1 receptors. Here, we present the high-resolution structure of netrin-4, which shows unique features in comparison with netrin-1, and show that it does not bind directly to any of the known netrin-1 receptors. We show that netrin-4 disrupts laminin networks and basement membranes (BMs) through high-affinity binding to the laminin γ1 chain. We hypothesize that this laminin-related function is essential for the previously described effects on axon growth promotion and angiogenesis. Our study unveils netrin-4 as a non-enzymatic extracellular matrix protein actively disrupting pre-existing BMs. PMID:27901020

A reworked Lake Zone margin: Chronological and geochemical constraints from the Ordovician arc-related basement of the Hovd Zone (western Mongolia)

NASA Astrophysics Data System (ADS)

Soejono, Igor; Buriánek, David; Janoušek, Vojtěch; Svojtka, Martin; Čáp, Pavel; Erban, Vojtěch; Ganpurev, Nyamtsetseg

2017-12-01

The primary relationships and character of the boundaries between principal lithotectonic domains in the Mongolian tract of the Central Asian Orogenic Belt (CAOB) are still poorly constrained. This brings much uncertainty in understanding of the orogeny configuration and the complete accretionary history. The plutonic Khuurai Tsenkher Gol Complex and the mainly metasedimentary Bij Group represent associated medium- to high-grade basement complexes exposed in the Hovd Zone close to its boundary with the Lake Zone in western Mongolia. The Khuurai Tsenkher Gol Complex is composed of variously deformed acid to basic magmatic rocks intimately associated with the metamorphosed sedimentary and volcanic rocks of the Bij Group. Results of our field work, new U-Pb zircon ages and whole-rock geochemical data suggest an existence of two separate magmatic events within the evolution of the Khuurai Tsenkher Gol Complex. Early to Mid-Ordovician (476 ± 5 Ma and 467 ± 4 Ma protoliths) normal- to high-K calc-alkaline orthogneisses, metadiorites and metagabbros predominate over Mid-Silurian (430 ± 3 Ma) tholeiitic-mildly alkaline quartz monzodiorites. Whereas the geochemical signature of the former suite unequivocally demonstrates its magmatic-arc origin, that of the latter quartz monzodiorite suggests an intra-plate setting. As shown by Sr-Nd isotopic data, the older arc-related magmas were derived from depleted mantle and/or were generated by partial melting of juvenile metabasic crust. Detrital zircon age populations of the metasedimentary rocks together with geochemical signatures of the associated amphibolites imply that the Bij Group was a volcano-sedimentary sequence, formed probably in the associated fore-arc wedge basin. Moreover, our data argue for an identical provenance of the Altai and Hovd domains, overall westward sediment transport during the Early Palaeozoic and the east-dipping subduction polarity. The obvious similarities of the Khuurai Tsenkher Gol Complex

Structural analysis of the Hasan-Robat marbles as traces of folded basement in the Sanandaj-Sirjan Zone, Iran

NASA Astrophysics Data System (ADS)

Nadimi, Alireza

2015-11-01

Cherty marbles of Hasan-Robat area, northwest of Isfahan, in the Sanandaj-Sirjan Zone of Iran preserves evidences of multiple deformational events. The Sanandaj-Sirjan Zone is the inner crystalline zone of the Zagros Orogen, which has been highly deformed and exhumed during continental collision between the Arabian Plate and Central Iran. The Hasan-Robat area is an example of the exposed Precambrian-Paleozoic basement rocks that stretched along two NW-SE-trending faults and located in the inner part of the HasanRobat positive flower strcuture. The Hasan-Robat marbles record a complex shortening and shearing history. This lead to the development of disharmonic ptygmatic folds with vertical to sub-vertical axes and some interference patterns of folding that may have been created from deformations during the Pan-African Orogeny and later phases. Based on this research, tectonic evolution of the Hasan-Robat area is interpreted as the product of three major geotectonic events that have been started after Precambrian to Quaternary: (1) old deformation phases (2) contractional movements and (3) strike-slip movements. Different sets and distributions of joints, faults and folds are confirmed with effect of several deformational stages of the area and formation of the flower structure.

Effect of basement structure and salt tectonics on deformation styles along strike: An example from the Kuqa fold-thrust belt, West China

NASA Astrophysics Data System (ADS)

Neng, Yuan; Xie, Huiwen; Yin, Hongwei; Li, Yong; Wang, Wei

2018-04-01

The Kuqa fold-thrust belt (KFTB) has a complex thrust-system geometry and comprises basement-involved thrusts, décollement thrusts, triangle zones, strike-slip faults, transpressional faults, and pop-up structures. These structures, combined with the effects of Paleogene salt tectonics and Paleozoic basement uplift form a complex structural zone trending E-W. Interpretation and comprehensive analysis of recent high-quality seismic data, field observations, boreholes, and gravity data covering the KFTB has been performed to understand the characteristics and mechanisms of the deformation styles along strike. Regional sections, fold-thrust system maps of the surface and the sub-salt layer, salt and basement structure distribution maps have been created, and a comprehensive analysis of thrust systems performed. The results indicate that the thrust-fold system in Paleogene salt range can be divided into five segments from east to west: the Kela-3, Keshen, Dabei, Bozi, and Awate segments. In the easternmost and westernmost parts of the Paleogene salt range, strike-slip faulting and basement-involved thrusting are the dominant deformation styles, as basement uplift and the limits of the Cenozoic evaporite deposit are the main controls on deformation. Salt-core detachment fold-thrust systems coincide with areas of salt tectonics, and pop-up, imbricate, and duplex structures are associated with the main thrust faults in the sub-salt layer. Distribution maps of thrust systems, basement structures, and salt tectonics show that Paleozoic basement uplift controlled the Paleozoic foreland basin morphology and the distribution of Cenozoic salt in the KFTB, and thus had a strong influence on the segmented structural deformation and evolution of the fold-thrust belt. Three types of transfer zone are identified, based on the characteristics of the salt layer and basement uplift, and the effects of these zones on the fault systems are evaluated. Basement uplift and the boundary of

Histones have high affinity for the glomerular basement membrane. Relevance for immune complex formation in lupus nephritis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmiedeke, T.M.; Stoeckl, F.W.W.; Weber, R.

1989-06-01

An effort has been made to integrate insights on charge-based interactions in immune complex glomerulonephritis with nuclear antigen involvement in lupus nephritis. Attention was focussed on the histones, a group of highly cationic nuclear constituents, which could be expected to bind to fixed anionic sites present in the glomerular basement membrane (GBM). We demonstrated that all histone subfractions, prepared according to Johns, have a high affinity for GBM and the basement membrane of peritubular capillaries. Tissue uptake of /sup 125/I-labeled histones was measured by injecting 200 micrograms of each fraction into the left kidney via the aorta and measuring organmore » uptake after 15 min. In glomeruli isolated from the left kidneys, the following quantities of histones were found: f1, 13 micrograms; f2a (f2al + f2a2), 17 micrograms; f2b, 17 micrograms; and f3, 32 micrograms. Kinetic studies of glomerular binding showed that f1 disappeared much more rapidly than f2a. The high affinity of histones (pI between 10.5 and 11.0; mol wt 10,000-22,000) for the GBM correlates well with their ability to form aggregates (mol wt greater than 100,000) for comparison lysozyme (pI 11, mol wt 14,000), which does not aggregate spontaneously bound poorly (0.4 micrograms in isolated glomeruli). The quantity of histones and lysozyme found in the isolated glomeruli paralleled their in vitro affinity for a Heparin-Sepharose column (gradient elution studies). This gel matrix contains the sulfated, highly anionic polysaccharide heparin, which is similar to the negatively charged heparan sulfate present in the GBM. Lysozyme eluted with 0.15 M NaCl, f1 with 1 M NaCl, and f2a, f2b, and f3 could not be fully desorbed even with 2 M NaCl; 6 M guanidine-HCl was necessary.« less

Dillon cutoff-Basement-involved tectonic link between the disturbed belt of west-central Montana and the overthrust belt of extreme southwestern Montana

NASA Astrophysics Data System (ADS)

O'Neill, J. Michael; Schmidt, Christopher J.; Genovese, Paul W.

1990-11-01

The front of the Cordilleran fold and thrust belt in western Montana follows the disturbed belt in the north, merges with the southwest Montana transverse zone in the west-central part of the region, and in southwestern Montana is marked by a broad zone characterized by complex interaction between thrust belt structures and basement uplifts. The front margin of the thrust belt in Montana reflects mainly thin-skinned tectonic features in the north, an east-trending lateral ramp that curves southwest in the central part into the Dillon cutoff, an oblique-slip, thick-skinned displacement transfer zone that cuts through basement rocks of the Lima recess, and a zone of overlap between thin- and thick-skinned thrusts in extreme southwestern Montana. The transverse ramp and basement-involved thrust faults are controlled by Proterozoic structures.

Disruption of basement membrane, extracellular matrix metalloproteinases and E-cadherin in renal-cell carcinoma.

PubMed

Morell-Quadreny, L; Rubio, Jose; Lopez-Guerrero, Jose Antonio; Casanova, Juan; Ramos, D; Iborra, Inmaculada; Solsona, Eduardo; Llombart-Bosch, A

2003-01-01

A retrospective study was performed to determine the prognostic value of Basement Membrane (BM) integrity, Matrix Metalloproteinases (MMPs) and E-Cadherin expression in renal cell carcinoma (RCC). An immunohistochemical study on laminin and collagen IV, MMPs 1 and 2, and E-Cadherin was carried out on 71 RCCs. BM fragmentation was considered taking 75% as a cut-off. MMP 1 and MMP2 immunostaining, as well as E-Cadherin was considered taking 25% as a cut-off. An inverse relationship was seen between E-Cadherin with laminin, collagen IV and MMPs. More than 75% loss of laminin, collagen IV and E-Cadherin, as well as higher expression of MMPs, were associated with symptoms, tumoral size and worse grade. Loss of collagen IV and E-Cadherin were of prognostic value. Both BM and E-Cadherin are good prognostic markers. MMPs patterns show a relationship between BM proteins and E-Cadherin, but evaluation is more time-consuming and provide no better prognostication; consequently they are not useful in routine clinical applications.

Mapping structural landmarks, ligand binding sites and missense mutations to the collagen IV heterotrimers predicts major functional domains, novel interactions and variation in phenotypes in inherited diseases affecting basement membranes

PubMed Central

Des Parkin, J.; San Antonio, James D.; Pedchenko, Vadim; Hudson, Billy; Jensen, Shane T.; Savige, Judy

2016-01-01

Collagen IV is the major protein found in basement membranes. It comprises 3 heterotrimers (α1α1α2, α3α4α5, and α5α5α6) that form distinct networks, and are responsible for membrane strength and integrity. We constructed linear maps of the collagen IV heterotrimers (‘interactomes’) that indicated major structural landmarks, known and predicted ligand-binding sites, and missense mutations, in order to identify functional and disease-associated domains, potential interactions between ligands, and genotype-phenotype relationships. The maps documented more than 30 known ligand-binding sites as well as motifs for integrins, heparin, von Willebrand factor (VWF), decorin and bone morphogenetic protein (BMP). They predicted functional domains for angiogenesis and haemostasis, and disease domains for autoimmunity, tumor growth and inhibition, infection and glycation. Cooperative ligand interactions were indicated by binding site proximity, for example, between integrins, matrix metalloproteinases and heparin. The maps indicated that mutations affecting major ligand-binding sites, for example for Von Hippel Lindau (VHL) protein in the α1 chain or integrins in the α5 chain, resulted in distinctive phenotypes (Hereditary Angiopathy, Nephropathy, Aneurysms and muscle Cramps (HANAC) syndrome, and early onset Alport syndrome respectively). These maps further our understanding of basement membrane biology and disease, and suggest novel membrane interactions, functions, and therapeutic targets. PMID:21280145

Reticular basement membrane in asthma and COPD: Similar thickness, yet different composition

PubMed Central

Liesker, Jeroen JW; Hacken, Nick H Ten; Zeinstra-Smith, Mieke; Rutgers, Steven R; Postma, Dirkje S; Timens, Wim

2009-01-01

Background Reticular basement membrane (RBM) thickening has been variably associated with asthma and chronic obstructive pulmonary disease (COPD). Even if RBM thickness is similar in both diseases, its composition might still differ. Objective To assess whether RBM thickness and composition differ between asthma and COPD. Methods We investigated 24 allergic asthmatics (forced expiratory volume in one second [FEV1] 92% predicted), and 17 nonallergic COPD patients (FEV1 60% predicted), and for each group a control group of similar age and smoking habits (12 and 10 persons, respectively). Snap-frozen sections of bronchial biopsies were stained with hematoxylin/eosin and for collagen I, III, IV, V, laminin and tenascin. RBM thickening was assessed by digital image analysis. Relative staining intensity of each matrix component was determined. Results Mean (SD) RBM thickness was not significantly different between asthma and COPD 5.5 (1.3) vs 6.0 (1.8) μm, but significantly larger than in their healthy counterparts, ie, 4.7 (0.9) and 4.8 (1.2) μm, respectively. Collagen I and laminin stained significantly stronger in asthma than in COPD. Tenascin stained stronger in asthma than in healthy controls of similar age, and stronger in COPD controls than in asthma controls (p < 0.05). Conclusion RBM thickening occurs both in asthma and COPD. We provide supportive evidence that its composition differs in asthma and COPD. PMID:19436691

APPALACHIAN FOLDS, LATERAL RAMPS, AND BASEMENT FAULTS: A MODERN ENGINEERING PROBLEM?

USGS Publications Warehouse

Pohn, Howard A.

1987-01-01

Field studies and analysis of radar data have shown that cross-strike faulting in the central and southern Appalachians has affected geologic structures at the surface. These basement faults appear to have been active through much of geologic time. Indeed, more than 45 percent of modern earthquakes occur along these narrow zones here termed 'lateral ramps. ' Because of this seismic activity, these lateral ramps are likely to be zones that are prone to slope failure. The engineer should be aware of the presence of such zones and the higher landslide potential along them.

Antibody localization in the glomerular basement membrane may precede in situ immune deposit formation in rat glomeruli.

PubMed

Agodoa, L Y; Gauthier, V J; Mannik, M

1985-02-01

The administration of cationized antibodies, specific to human serum albumin, into the renal artery of rats caused transient presence of IgG in glomeruli by immunofluorescence microscopy. Intravenous infusion of appropriate doses of antigen after the injection of cationized antibodies resulted in immune deposit formation in glomeruli that persisted through 96 hr. By electron microscopy, these deposits were located in the subepithelial area. The injection of large doses of antigen produced immune deposits which were present in glomeruli for only a few hours, presumably due to formation of only small-latticed immune complexes. The presented data indicate that cationic antibodies bound to the fixed negative charges of the glomerular basement membrane can interact with circulating antigen to form immune deposits in glomeruli. This mechanism may be important because anionic antigens have been shown to induce the synthesis of cationic antibodies.

Protective and Heat Retention Effects of Thermo-sensitive Basement Membrane Extract (Matrigel) in Hepatic Radiofrequency Ablation in an Experimental Animal Study.

PubMed

Fu, Jing-Jing; Wang, Song; Yang, Wei; Gong, Wei; Jiang, An-Na; Yan, Kun; Chen, Min-Hua

2017-07-01

To evaluate the protective effect of using thermo-sensitive basement membrane extract (Matrigel) for hydrodissection to minimize thermal injury to nearby structures and to evaluate its heat sink effect on the ablation zone in radiofrequency ablation (RFA) of the liver. First, the viscosity profile and heat sink effect of Matrigel were assessed during RFA in vitro and ex vivo. Fresh pig liver tissue was used, and the temperature changes in Matrigel and in 5% dextrose in water (D5W) during RFA were recorded. Then, the size of the ablation zone in the peripheral liver after RFA was measured. Second, in an in vivo study, 45 Sprague-Dawley rats were divided into three groups of 15 rats each (Matrigel, D5W and control). In the experimental groups, artificial ascites with 10 ml of Matrigel or D5W were injected using ultrasound guidance prior to RFA. The frequency of thermal injury to the nearby organs was compared among the three groups, with assessments of several locations: near the diaphragm, the abdominal wall and the gastrointestinal (GI) tract. Finally, the biological degradation of Matrigel by ultrasound was evaluated over 60 days. First, Matrigel produced a greater heat retention (less heat sink) effect than D5W during ex vivo ablation (63 ± 9 vs. 26 ± 6 °C at 1 min on the surface of the liver, P < 0.001). Hepatic ablation zone volume did not differ between the two groups. Second, thermal injury to the nearby structures was found in 14 of 15 cases (93.3%) in the control group, 8 of 15 cases (53.3%) in the D5W group, and 1 of 15 cases (6.7%) in the Matrigel group. Significant differences in the thermal injury rates for nearby structures were detected among the three groups (P < 0.001). The most significant difference in the thermal injury rate was found in locations near the GI tract (P = 0.003). Finally, Matrigel that was injected in vivo was gradually degraded during the following 60 days. Using thermo-sensitive Matrigel as a hydrodissection

A Review of String Vessels or Collapsed, Empty Basement Membrane Tubes

PubMed Central

Brown, William R.

2011-01-01

String vessels are thin connective tissue strands, remnants of capillaries, with no endothelial cells; they do not carry blood flow. They occur in numerous species, particularly in the central nervous system, but can occur in any tissue where capillaries have died. String vessels are often associated with pathologies such as Alzheimer’s disease, ischemia, and irradiation, but are also found in normal human brains from preterm babies to the aged. They provide a record of the original blood vessel location, but gradually disappear after months or years. There have been numerous studies of string vessels (acellular capillaries) in the retina, because retinal vessels can be seen in great detail in whole mounts after trypsin digestion. Capillary regression occurs by apoptosis, synchronously along capillary segments, with macrophages engulfing apoptotic endothelial cells. Macrophages may cause the apoptosis, or the regression may be triggered by loss of the endothelial cell survival factor VEGF. VEGF expression is induced by hypoxia and promotes capillary growth. Cessation of blood flow eliminates the shear stress that helps maintain endothelial cell survival. Capillaries can re-grow by proliferation and migration of endothelial cells into empty basement membrane tubes, which provide a structural scaffold, replete with signaling molecules. This is a problem in tumor control, but useful for recovery from capillary loss. There is an age-related waning of VEGF expression in response to hypoxia. This causes an age-related decline in cerebral angiogenesis and results in neuronal loss. It may also contribute to the proposed age-related loss of brain reserve. PMID:20634580

Optimizing the design of vertical seismic profiling (VSP) for imaging fracture zones over hardrock basement geothermal environments

NASA Astrophysics Data System (ADS)

Reiser, Fabienne; Schmelzbach, Cedric; Maurer, Hansruedi; Greenhalgh, Stewart; Hellwig, Olaf

2017-04-01

A primary focus of geothermal seismic imaging is to map dipping faults and fracture zones that control rock permeability and fluid flow. Vertical seismic profiling (VSP) is therefore a most valuable means to image the immediate surroundings of an existing borehole to guide, for example, the placing of new boreholes to optimize production from known faults and fractures. We simulated 2D and 3D acoustic synthetic seismic data and processed it through to pre-stack depth migration to optimize VSP survey layouts for mapping moderately to steeply dipping fracture zones within possible basement geothermal reservoirs. Our VSP survey optimization procedure for sequentially selecting source locations to define the area where source points are best located for optimal imaging makes use of a cross-correlation statistic, by which a subset of migrated shot gathers is compared with a target or reference image from a comprehensive set of source gathers. In geothermal exploration at established sites, it is reasonable to assume that sufficient à priori information is available to construct such a target image. We generally obtained good results with a relatively small number of optimally chosen source positions distributed over an ideal source location area for different fracture zone scenarios (different dips, azimuths, and distances from the surveying borehole). Adding further sources outside the optimal source area did not necessarily improve the results, but rather resulted in image distortions. It was found that fracture zones located at borehole-receiver depths and laterally offset from the borehole by 300 m can be imaged reliably for a range of the different dips, but more source positions and large offsets between sources and the borehole are required for imaging steeply dipping interfaces. When such features cross-cut the borehole, they are particularly difficult to image. For fracture zones with different azimuths, 3D effects are observed. Far offset source positions

Active Deformation of Malawi Rift's North Basin Hinge Zone Modulated by Reactivation of Preexisting Precambrian Shear Zone Fabric

NASA Astrophysics Data System (ADS)

Kolawole, F.; Atekwana, E. A.; Laó-Dávila, D. A.; Abdelsalam, M. G.; Chindandali, P. R.; Salima, J.; Kalindekafe, L.

2018-03-01

We integrated temporal aeromagnetic data and recent earthquake data to address the long-standing question on the role of preexisting Precambrian structures in modulating strain accommodation and subsequent ruptures leading to seismic events within the East African Rift System. We used aeromagnetic data to elucidate the relationship between the locations of the 2009 Mw 6.0 Karonga, Malawi, earthquake surface ruptures and buried basement faults along the hinge zone of the half-graben comprising the North Basin of the Malawi Rift. Through the application of derivative filters and depth-to-magnetic-source modeling, we identified and constrained the trend of the Precambrian metamorphic fabrics and correlated them to the three-dimensional structure of buried basement faults. Our results reveal an unprecedented detail of the basement fabric dominated by high-frequency WNW to NW trending magnetic lineaments associated with the Precambrian Mughese Shear Zone fabric. The high-frequency magnetic lineaments are superimposed by lower frequency NNW trending magnetic lineaments associated with possible Cenozoic faults. Surface ruptures associated with the 2009 Mw 6.0 Karonga earthquake swarm aligned with one of the NNW-trending magnetic lineaments defining a normal fault that is characterized by right-stepping segments along its northern half and coalesced segments on its southern half. Fault geometries, regional kinematics, and spatial distribution of seismicity suggest that seismogenic faults reactivated the basement fabric found along the half-graben hinge zone. We suggest that focusing of strain accommodation and seismicity along the half-graben hinge zone is facilitated and modulated by the presence of the basement fabric.

Influence of pre-existing basement faults on the structural evolution of the Zagros Simply Folded belt: 3D numerical modelling

NASA Astrophysics Data System (ADS)

Ruh, Jonas B.; Gerya, Taras

2015-04-01

The Simply Folded Belt of the Zagros orogen is characterized by elongated fold trains symptomatically defining the geomorphology along this mountain range. The Zagros orogen results from the collision of the Arabian and the Eurasian plates. The Simply Folded Belt is located southwest of the Zagros suture zone. An up to 2 km thick salt horizon below the sedimentary sequence enables mechanical and structural detachment from the underlying Arabian basement. Nevertheless, deformation within the basement influences the structural evolution of the Simply Folded Belt. It has been shown that thrusts in form of reactivated normal faults can trigger out-of-sequence deformation within the sedimentary stratigraphy. Furthermore, deeply rooted strike-slip faults, such as the Kazerun faults between the Fars zone in the southeast and the Dezful embayment and the Izeh zone, are largely dispersing into the overlying stratigraphy, strongly influencing the tectonic evolution and mechanical behaviour. The aim of this study is to reveal the influence of basement thrusts and strike-slip faults on the structural evolution of the Simply Folded Belt depending on the occurrence of intercrustal weak horizons (Hormuz salt) and the rheology and thermal structure of the basement. Therefore, we present high-resolution 3D thermo-mechnical models with pre-existing, inversively reactivated normal faults or strike-slip faults within the basement. Numerical models are based on finite difference, marker-in-cell technique with (power-law) visco-plastic rheology accounting for brittle deformation. Preliminary results show that deep tectonic structures present in the basement may have crucial effects on the morphology and evolution of a fold-and-thrust belt above a major detachment horizon.

Role for Macrophage Metalloelastase in Glomerular Basement Membrane Damage Associated with Alport Syndrome

PubMed Central

Rao, Velidi H.; Meehan, Daniel T.; Delimont, Duane; Nakajima, Motowo; Wada, Takashi; Ann Gratton, Michael; Cosgrove, Dominic

2006-01-01

Alport syndrome is a glomerular basement membrane (GBM) disease caused by mutations in type IV collagen genes. A unique irregular thickening and thinning of the GBM characterizes the progressive glomerular pathology. The metabolic imbalances responsible for these GBM irregularities are not known. Here we show that macrophage metalloelastase (MMP-12) expression is >40-fold induced in glomeruli from Alport mice and is markedly induced in glomeruli of both humans and dogs with Alport syndrome. Treatment of Alport mice with MMI270 (CGS27023A), a broad spectrum MMP inhibitor that blocks MMP-12 activity, results in largely restored GBM ultrastructure and function. Treatment with BAY-129566, a broad spectrum MMP inhibitor that does not inhibit MMP-12, had no effect. We show that inhibition of CC chemokine receptor 2 (CCR2) receptor signaling with propagermanium blocks induction of MMP-12 mRNA and prevents GBM damage. CCR2 receptor is expressed in glomerular podocytes of Alport mice, suggesting MCP-1 activation of CCR2 on podocytes may underlie induction of MMP-12. These data indicate that the irregular GBM that characterizes Alport syndrome may be mediated, in part, by focal degradation of the GBM due to MMP dysregulation, in particular, MMP-12. Thus, MMP-12/CCR2 inhibitors may provide a novel and effective therapeutic strategy for Alport glomerular disease. PMID:16816359

Airway basement membrane perimeter in human airways is not a constant; potential implications for airway remodeling in asthma.

PubMed

McParland, Brent E; Paré, Peter D; Johnson, Peter R A; Armour, Carol L; Black, Judith L

2004-08-01

Many studies that demonstrate an increase in airway smooth muscle in asthmatic patients rely on the assumption that bronchial internal perimeter (P(i)) or basement membrane perimeter (P(bm)) is a constant, i.e., not affected by fixation pressure or the degree of smooth muscle shortening. Because it is the basement membrane that has been purported to be the indistensible structure, this study examines the assumption that P(bm) is not affected by fixation pressure. P(bm) was determined for the same human airway segment (n = 12) fixed at distending pressures of 0 cmH(2)O and 21 cmH(2)O in the absence of smooth muscle tone. P(bm) for the segment fixed at 0 cmH(2)O was determined morphometrically, and the P(bm) for the same segment, had the segment been fixed at 21 cmH(2)O, was predicted from knowing the luminal volume and length of the airway when distended to 21 cmH(2)O (organ bath-derived P(i)). To ensure an accurate transformation of the organ bath-derived P(i) value to a morphometry-derived P(bm) value, had the segment been fixed at 21 cmH(2)O, the relationship between organ bath-derived P(i) and morphometry-derived P(bm) was determined for five different bronchial segments distended to 21 cmH(2)O and fixed at 21 cmH(2)O (r(2) = 0.99, P < 0.0001). Mean P(bm) for bronchial segments fixed at 0 cmH(2)O was 9.4 +/- 0.4 mm, whereas mean predicted P(bm), had the segments been fixed at 21 cmH(2)O, was 14.1 +/- 0.5 mm (P < 0.0001). This indicates that P(bm) is not a constant when isolated airway segments without smooth muscle tone are fixed distended to 21 cmH(2)O. The implication of these results is that the increase in smooth muscle mass in asthma may have been overestimated in some previous studies. Therefore, further studies are required to examine the potential artifact using whole lungs with and without abolition of airway smooth muscle tone and/or inflation.

Expression and organization of basement membranes and focal adhesion proteins in pregnant myometrium is regulated by uterine stretch.

PubMed

Shynlova, Oksana; Chow, Michelle; Lye, Stephen J

2009-10-01

The mechanisms underlying the preparation of the uterus for labor are not fully understood. We have previously found a significant increase in the expression of messenger RNA (mRNAs) encoding extracellular basement membrane (BM) proteins of the smooth muscle cells (SMCs) in late pregnant rat myometrium. At term, the myometrium is stretched by growing fetuses and these mechanical signals are transmitted from extracellular matrix into SMCs through focal adhesions (FA). The aim of this study was to investigate the effect of gravidity on the expression and spatiotemporal distribution of major BM proteins, laminin-gamma2 and collagen IV, as well as typical FA constituents, vinculin and paxillin, in the myometrium during gestation and parturition, using a unilaterally pregnant rat model. We found that the expression of laminin-gamma2 and collagen IV proteins increased significantly with gestational age (P < .05) and was dependent on gravidity whereas vinculin and paxillin proteins were not affected. Near term, BM proteins from gravid horn myometrium demonstrated increased extracellular immunostaining and major rearrangement from sporadic protein distribution to organized, continuous, and regular structures surrounding the plasma membrane of each myocyte. Examination of FA proteins revealed that paxillin was translocated from the cytoplasm to the cell periphery, while vinculin was sequestered specifically to FAs. At labor, BM and FA proteins, organized in similar bead-like structures, were localized on opposing sides of SMC plasma membrane into 2 different compartments. We suggest that these stretch-induced changes facilitate formation of stable cell-matrix adhesions and provide the molecular basis for optimal force transduction during labor contractions.

Basement structures over Rio Grande Rise from gravity inversion

NASA Astrophysics Data System (ADS)

Constantino, Renata; Hackspacker, Peter Christian; Anderson de Souza, Iata; Sousa Lima Costa, Iago

2017-04-01

In this study, we show that from satellite-derived gravity field, bathymetry and sediment thicknesses, it is possible to give a 3-D model of the basement over oceanic areas, and for this purpose, we have chosen the Rio Grande Rise, in South Atlantic Ocean, to build a gravity-equivalent basement topography. The advantages of the method applied in this study are manifold: does not depend directly on reflection seismic data; can be applied quickly and with fewer costs for acquiring and interpreting the data; and as the main result, presents the physical surface below the sedimentary layer, which may be different from the acoustic basement. We evaluated the gravity effect of the sediments using the global sediment thickness model of NOAA, fitting a sediment compaction model to observed density values from Deep Sea Drilling Program (DSDP) reports. The Global Relief Model ETOPO1 and constraining data from seismic interpretation on crustal thickness are integrated in the gravity inversion procedure. The modeled Moho depth values vary between 6 to 27 km over the area, being thicker under the Rio Grande Rise and also in the direction of São Paulo Plateau. The inversion for the gravity-equivalent basement topography is applied for a gravity residual data, which is free from the gravity effect of sediments and from the gravity effect of the estimated Moho interface. A description of the basement depth over Rio Grande Rise area is unprecedented in the literature, however, our results could be compared to in situ data, provided by DSDP, and a small difference of only 9 m between our basement depth and leg 516 F was found. Our model shows a rift crossing the entire Rio Grande Rise deeper than previously presented in literature, with depths up to 5 km in the East Rio Grande Rise (ERGR) and deeper in the West Rio Grande Rise (WRGR), reaching 6.4 km. We find several short-wavelengths structures not present in the bathymetry data. Seamounts, guyots and fracture zones are much more

Control of Precambrian basement deformation zones on emplacement of the Laramide Boulder batholith and Butte mining district, Montana, United States

USGS Publications Warehouse

Berger, Byron R.; Hildenbrand, Thomas G.; O'Neill, J. Michael

2011-01-01

What are the roles of deep Precambrian basement deformation zones in the localization of subsequent shallow-crustal deformation zones and magmas? The Paleoproterozoic Great Falls tectonic zone and its included Boulder batholith (Montana, United States) provide an opportunity to examine the importance of inherited deformation fabrics in batholith emplacement and the localization of magmatic-hydrothermal mineral deposits. Northeast-trending deformation fabrics predominate in the Great Falls tectonic zone, which formed during the suturing of Paleoproterozoic and Archean cratonic masses approximately 1,800 mega-annum (Ma). Subsequent Mesoproterozoic to Neoproterozoic deformation fabrics trend northwest. Following Paleozoic through Early Cretaceous sedimentation, a Late Cretaceous fold-and-thrust belt with associated strike-slip faulting developed across the region, wherein some Proterozoic faults localized thrust faulting, while others were reactivated as strike-slip faults. The 81- to 76-Ma Boulder batholith was emplaced along the reactivated central Paleoproterozoic suture in the Great Falls tectonic zone. Early-stage Boulder batholith plutons were emplaced concurrent with east-directed thrust faulting and localized primarily by northwest-trending strike-slip and related faults. The late-stage Butte Quartz Monzonite pluton was localized in a northeast-trending pull-apart structure that formed behind the active thrust front and is axially symmetric across the underlying northeast-striking Paleoproterozoic fault zone, interpreted as a crustal suture. The modeling of potential-field geophysical data indicates that pull-apart?stage magmas fed into the structure through two funnel-shaped zones beneath the batholith. Renewed magmatic activity in the southern feeder from 66 to 64 Ma led to the formation of two small porphyry-style copper-molybdenum deposits and ensuing world-class polymetallic copper- and silver-bearing veins in the Butte mining district. Vein orientations

7. VIEW OF BASEMENT, LOOKING NORTH ALONG EAST BASEMENT WALL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. VIEW OF BASEMENT, LOOKING NORTH ALONG EAST BASEMENT WALL TOWARD TURBINES. AT RIGHT IS A WATER-POWERED EAR CORN CRUSHER (manufacturer unknown), WHICH PERFORMED THE INITIAL COARSE GRINDING OF EAR CORN Photographer: Jet T. Lowe, 1985 - Alexander's Grist Mill, Lock 37 on Ohio & Erie Canal, South of Cleveland, Valley View, Cuyahoga County, OH

Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix.

PubMed

Kehlet, Stephanie N; Willumsen, Nicholas; Armbrecht, Gabriele; Dietzel, Roswitha; Brix, Susanne; Henriksen, Kim; Karsdal, Morten A

2018-01-01

The extracellular matrix (ECM) plays a vital role in maintaining normal tissue function. Collagens are major components of the ECM and there is a tight equilibrium between degradation and formation of these proteins ensuring tissue health and homeostasis. As a consequence of tissue turnover, small collagen fragments are released into the circulation, which act as important biomarkers in the study of certain tissue-related remodeling factors in health and disease. The aim of this study was to establish an age-related collagen turnover profile of the main collagens of the interstitial matrix (type I and III collagen) and basement membrane (type IV collagen) in healthy men and women. By using well-characterized competitive ELISA-assays, we assessed specific fragments of degraded (C1M, C3M, C4M) and formed (PINP, Pro-C3, P4NP7S) type I, III and IV collagen in serum from 617 healthy men and women ranging in ages from 22 to 86. Subjects were divided into 5-year age groups according to their sex and age. Groups were compared using Kruskal-Wallis adjusted for Dunn's multiple comparisons test and Mann-Whitney t-test. Age-specific changes in collagen turnover was most profound for type I collagen. PINP levels decreased in men with advancing age, whereas in women, the level decreased in early adulthood followed by an increase around the age of menopause (age 40-60). Sex-specific changes in type I, III and IV collagen turnover was present at the age around menopause (age 40-60) with women having an increased turnover. In summary, collagen turnover is affected by age and sex with the interstitial matrix and the basement membrane being differently regulated. The observed changes needs to be accounted for when measuring ECM related biomarkers in clinical studies.

Fault-Slip Data Analysis and Cover Versus Basement Fracture Patterns - Implications for Subsurface Technical Processes in Thuringia, Germany

NASA Astrophysics Data System (ADS)

Kasch, N.; Kley, J.; Navabpour, P.; Siegburg, M.; Malz, A.

2014-12-01

Recent investigations in Thuringia, Central Germany, focus on the potential for carbon sequestration, groundwater supply and geothermal energy. We report on the results of an integrated fault-slip data analysis to characterize the geometries and kinematics of systematic fractures in contrasting basement and cover rock lithologies. The lithostratigraphy of the area comprises locally exposed crystalline rocks and intermittently overlying Permian volcanic and clastic sedimentary rocks, together referred to as basement. A Late Permian sequence of evaporites, carbonates and shale constitutes the transition to the continuous sedimentary cover of Triassic age. Major NW-SE-striking fault zones and minor NNE-SSW-striking faults affect this stratigraphic succession. These characteristic narrow deforming areas (< 3 km width) build a dense network of individual fault strands with a close juxtaposition to wider (> 15 km) non-deforming areas suggesting localized zones of mechanical weakness, which can be confirmed by the frequent reactivation of single fault strands. Along the major fault zones, the basement and cover contain dominant inclined to sub-vertical NW-SE-striking fractures. These fractures indicate successive normal, dextral strike-slip and reverse senses of slip, evidencing events of NNE-SSW extension and contraction. Another system of mostly sub-vertical NNW-SSE- and NE-SW-striking conjugate strike-slip faults mainly developed within the cover implies NNE-SSW contraction and WNW-ESE extension. Earthquake focal mechanisms and in-situ stress measurements reveal a NW-SE trend for the modern SHmax. Nevertheless, fractures and fault-slip indicators are rare in the non-deforming areas, which characterizes Thuringia as a dual domain of (1) large unfractured areas and (2) narrow zones of high potential for technical applications. Our data therefore provide a basis for estimation of slip and dilation tendency of the contrasting fractures in the basement and cover under the

NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone

PubMed Central

Awata, Junya; Takada, Saeko; Standley, Clive; Lechtreck, Karl F.; Bellvé, Karl D.; Pazour, Gregory J.; Fogarty, Kevin E.; Witman, George B.

2014-01-01

ABSTRACT The protein nephrocystin-4 (NPHP4) is widespread in ciliated organisms, and defects in NPHP4 cause nephronophthisis and blindness in humans. To learn more about the function of NPHP4, we have studied it in Chlamydomonas reinhardtii. NPHP4 is stably incorporated into the distal part of the flagellar transition zone, close to the membrane and distal to CEP290, another transition zone protein. Therefore, these two proteins, which are incorporated into the transition zone independently of each other, define different domains of the transition zone. An nphp4-null mutant forms flagella with nearly normal length, ultrastructure and intraflagellar transport. When fractions from isolated wild-type and nphp4 flagella were compared, few differences were observed between the axonemes, but the amounts of certain membrane proteins were greatly reduced in the mutant flagella, and cellular housekeeping proteins >50 kDa were no longer excluded from mutant flagella. Therefore, NPHP4 functions at the transition zone as an essential part of a barrier that regulates both membrane and soluble protein composition of flagella. The phenotypic consequences of NPHP4 mutations in humans likely follow from protein mislocalization due to defects in the transition zone barrier. PMID:25150219

22. VIEW OF THE BASEMENT FLOOR PLAN. THE BASEMENT TUNNELS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. VIEW OF THE BASEMENT FLOOR PLAN. THE BASEMENT TUNNELS WERE DESIGNED AS FALLOUT SHELTERS AND USED FOR STORAGE. THE ORIGINAL DRAWING HAS BEEN ARCHIVED ON MICROFILM. THE DRAWING WAS REPRODUCED AT THE BEST QUALITY POSSIBLE. LETTERS AND NUMBERS IN THE CIRCLES INDICATE FOOTER AND/OR COLUMN LOCATIONS. - Rocky Flats Plant, General Manufacturing, Support, Records-Central Computing, Southern portion of Plant, Golden, Jefferson County, CO

Endurance exercise training decreases capillary basement membrane width in older nondiabetic and diabetic adults.

PubMed

Williamson, J R; Hoffmann, P L; Kohrt, W M; Spina, R J; Coggan, A R; Holloszy, O

1996-03-01

The objectives of these studies were to 1) evaluate the relationships among age, glucose intolerance, and skeletal muscle capillary basement membrane (CBM) width (CBMW) and 2) determine the effects of exercise training on CBMW by comparing values of young (28 +/- 4 yr) and older (63 +/- 7 yr) athletes with those of age-matched sedentary control subjects and by measuring CBMW in older men and women before and after a 9-mo endurance-exercise training program. CBMW was measured in tissue samples obtained from the gastrocnemius muscle. CBMW in sedentary 64 +/- 3-yr-old subjects was 25% thicker than in sedentary 24 +/- 3-yr-old subjects. CBMW was similar in young and older athletes and was thinner than the CBMW of age-matched sedentary control subjects. There were no differences in CBMW among older sedentary individuals with normal or impaired glucose tolerance or mild non-insulin-dependent diabetes mellitus. Nine months of endurance exercise training reduced CBMW in older men and women by 30-40%, to widths that were not different from those of the young subjects; this response was independent of glucose tolerance status. These findings suggest that habitual exercise prevents the thickening of the skeletal muscle CBM that is characteristic of advancing age. Moreover, the thickening of the CBM appears to be readily reversed as a result of exercise training, even in older individuals.

LOFT. Containment and service building (TAN650) basement floor plan. Basement ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LOFT. Containment and service building (TAN-650) basement floor plan. Basement airlock, shielded roadway, service areas, connection to control building. Kaiser engineers 6413-11-STEP/LOFT-650-A-1. Date: October 1964. INEEL index code no. 036-650-00-416-122213 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

[Morphology of basement membrane and associated matrix proteins in normal and pathological tissues].

PubMed

Nerlich, A

1995-01-01

Basement membranes (BM) are specialized structures of the extracellular matrix. Their composition is of particular importance for the maintenance of normal morphological and functional properties of a multitude of organs and tissue systems and it is thus required for regular homeostasis of body function. Generally, they possess three main functions, i.e. participation in the maintenance of tissue structure, control of fluid and substrate exchange, and regulation of cell growth and differentiation. BMs are made up by various components which are in part specifically localized within the BM zone, or which represent ubiquitous matrix constituents with specific quantitative and/or qualitative differences in their localization. On the basis of a thorough immunohistochemical analysis of normal and diseased tissues, we provide here a concept of "functional morphology/pathomorphology" of the different BM components analyzed: 1.) The ubiquitous BM-constituent collagen IV primarily stabilizes the BM-zone and thus represents the "backbone" of the BM providing mechanical strength. Its loss leads to cystic tissue transformation as it is evidenced from the analysis of polycystic nephropathies. Thus, in other cystic tissue transformations a similar formal pathogenesis may be present. 2.) The specific localization of collagen VII as the main structural component of anchoring fibrils underlines the mechanical anchoring function of this collagenous protein. Defects in this protein lead to hereditary epidermolysis. The rapid re-occurrence of epidermal collagen VII during normal human wound healing indicates a quick reconstitution of the mechanical tensile strength of healing wounds. 3.) The BM-specific heparan sulfate proteoglycan (HSPG, Perlecan) with its highly negative anionic charge can be assumed to exert filter control. This assumption is corroborated by the localizatory findings of a preferential deposition of HSPG in endothelial and particularly in glomerular BM. Similarly

A bioartificial environment for kidney epithelial cells based on a supramolecular polymer basement membrane mimic and an organotypical culture system.

PubMed

Mollet, Björne B; Bogaerts, Iven L J; van Almen, Geert C; Dankers, Patricia Y W

2017-06-01

Renal applications in healthcare, such as renal replacement therapies and nephrotoxicity tests, could potentially benefit from bioartificial kidney membranes with fully differentiated and functional human tubular epithelial cells. A replacement of the natural environment of these cells is required to maintain and study cell functionality cell differentiation in vitro. Our approach was based on synthetic supramolecular biomaterials to mimic the natural basement membrane (BM) on which these cells grow and a bioreactor to provide the desired organotypical culture parameters. The BM mimics were constructed from ureidopyrimidinone (UPy)-functionalized polymer and bioactive peptides by electrospinning. The resultant membranes were shown to have a hierarchical fibrous BM-like structure consisting of self-assembled nanofibres within the electrospun microfibres. Human kidney-2 (HK-2) epithelial cells were cultured on the BM mimics under organotypical conditions in a custom-built bioreactor. The bioreactor facilitated in situ monitoring and functionality testing of the cultures. Cell viability and the integrity of the epithelial cell barrier were demonstrated inside the bioreactor by microscopy and transmembrane leakage of fluorescently labelled inulin, respectively. Furthermore, HK-2 cells maintained a polarized cell layer and showed modulation of both gene expression of membrane transporter proteins and metabolic activity of brush border enzymes when subjected to a continuous flow of culture medium inside the new bioreactor for 21 days. These results demonstrated that both the culture and study of renal epithelial cells was facilitated by the bioartificial in vitro environment that is formed by synthetic supramolecular BM mimics in our custom-built bioreactor. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

A role for PDGF-C/PDGFRα signaling in the formation of the meningeal basement membranes surrounding the cerebral cortex

PubMed Central

Andrae, Johanna; Gouveia, Leonor; Gallini, Radiosa; He, Liqun; Fredriksson, Linda; Nilsson, Ingrid; Johansson, Bengt R.; Eriksson, Ulf; Betsholtz, Christer

2016-01-01

ABSTRACT Platelet-derived growth factor-C (PDGF-C) is one of three known ligands for the tyrosine kinase receptor PDGFRα. Analysis of Pdgfc null mice has demonstrated roles for PDGF-C in palate closure and the formation of cerebral ventricles, but redundancy with other PDGFRα ligands might obscure additional functions. In search of further developmental roles for PDGF-C, we generated mice that were double mutants for Pdgfc−/− and PdgfraGFP/+. These mice display a range of severe phenotypes including spina bifida, lung emphysema, abnormal meninges and neuronal over-migration in the cerebral cortex. We focused our analysis on the central nervous system (CNS), where PDGF-C was identified as a critical factor for the formation of meninges and assembly of the glia limitans basement membrane. We also present expression data on Pdgfa, Pdgfc and Pdgfra in the cerebral cortex and microarray data on cerebral meninges. PMID:26988758

Fluid flow and permeabilities in basement fault zones

NASA Astrophysics Data System (ADS)

Hollinsworth, Allan; Koehn, Daniel

2017-04-01

Fault zones are important sites for crustal fluid flow, specifically where they cross-cut low permeability host rocks such as granites and gneisses. Fluids migrating through fault zones can cause rheology changes, mineral precipitation and pore space closure, and may alter the physical and chemical properties of the host rock and deformation products. It is therefore essential to consider the evolution of permeability in fault zones at a range of pressure-temperature conditions to understand fluid migration throughout a fault's history, and how fluid-rock interaction modifies permeability and rheological characteristics. Field localities in the Rwenzori Mountains, western Uganda and the Outer Hebrides, north-west Scotland, have been selected for field work and sample collection. Here Archaean-age TTG gneisses have been faulted within the upper 15km of the crust and have experienced fluid ingress. The Rwenzori Mountains are an anomalously uplifted horst-block located in a transfer zone in the western rift of the East African Rift System. The north-western ridge is characterised by a tectonically simple western flank, where the partially mineralised Bwamba Fault has detached from the Congo craton. Mineralisation is associated with hydrothermal fluids heated by a thermal body beneath the Semliki rift, and has resulted in substantial iron oxide precipitation within porous cataclasites. Non-mineralised faults further north contain foliated gouges and show evidence of leaking fluids. These faults serve as an analogue for faults associated with the Lake Albert oil and gas prospects. The Outer Hebrides Fault Zone (OHFZ) was largely active during the Caledonian Orogeny (ca. 430-400 Ma) at a deeper crustal level than the Ugandan rift faults. Initial dry conditions were followed by fluid ingress during deformation that controlled its rheological behaviour. The transition also altered the existing permeability. The OHFZ is a natural laboratory in which to study brittle fault

Basement and cover-rock deformation during Laramide contraction in the northern Madison Range (Montana) and its influence on Cenozoic basin formation

USGS Publications Warehouse

Kellogg, K.S.; Schmidt, C.J.; Young, S.W.

1995-01-01

Two major Laramide fault systems converge in the northwestern Madison Range: the northwest-striking, southwest-vergent Spanish Peaks reverse fault and the north-striking, east-vergent Hilgard thrust system. Analysis of foliation attitudes in basement gneiss north and south of the Spanish Peaks fault indicates that the basement in thrusted blocks of the Hilgard thrust system have been rotated by an amount similar to that of the basement-cover contact. Steeply dipping, north-striking breccia zones enclosing domains of relatively undeformed basement may have permitted domino-style rotation of basement blocks during simple shear between pairs of thrusts. No hydrocarbon discoveries have been made in this unique structural province. However, petroleum exploration here has focused on basement-cored anticlines, both surface and subthrust, related to the two major Laramide fault systems and on the fault-bounded blocks of Tertiary rocks within the post-Laramide extensional basins. -from Authors

Integrating Reflection Seismic, Gravity and Magnetic Data to Reveal the Structure of Crystalline Basement: Implications for Understanding Rift Development

NASA Astrophysics Data System (ADS)

Lenhart, Antje; Jackson, Christopher A.-L.; Bell, Rebecca E.; Duffy, Oliver B.; Fossen, Haakon; Gawthorpe, Robert L.

2016-04-01

Numerous rifts form above crystalline basement containing pervasive faults and shear zones. However, the compositional and mechanical heterogeneity within crystalline basement and the geometry and kinematics of discrete and pervasive basement fabrics are poorly understood. Furthermore, the interpretation of intra-crustal structures beneath sedimentary basins is often complicated by limitations in the depth of conventional seismic imaging, the commonly acoustically transparent nature of basement, limited well penetrations, and complex overprinting of multiple tectonic events. Yet, a detailed knowledge of the structural and lithological complexity of crystalline basement rocks is crucial to improve our understanding of how rifts evolve. Potential field methods are a powerful but perhaps underutilised regional tool that can decrease interpretational uncertainty based solely on seismic reflection data. We use petrophysical data, high-resolution 3D reflection seismic volumes, gridded gravity and magnetic data, and 2D gravity and magnetic modelling to constrain the structure of crystalline basement offshore western Norway. Intra-basement structures are well-imaged on seismic data due to relatively shallow burial of the basement beneath a thin (<3.5 km) sedimentary cover. Variations in basement composition were interpreted from detailed seismic facies analysis and mapping of discrete intra-basement reflections. A variety of data filtering and isolation techniques were applied to the original gravity and magnetic data in order to enhance small-scale field variations, to accentuate formation boundaries and discrete linear trends, and to isolate shallow and deep crustal anomalies. In addition, 2D gravity and magnetic data modelling was used to verify the seismic interpretation and to further constrain the configuration of the upper and lower crust. Our analysis shows that the basement offshore western Norway is predominantly composed of Caledonian allochthonous nappes

New Mexico structural zone - An analogue of the Colorado mineral belt

USGS Publications Warehouse

Sims, P.K.; Stein, H.J.; Finn, C.A.

2002-01-01

Updated aeromagnetic maps of New Mexico together with current knowledge of the basement geology in the northern part of the state (Sangre de Cristo and Sandia-Manzano Mountains)-where basement rocks were exposed in Precambrian-cored uplifts-indicate that the northeast-trending Proterozoic shear zones that controlled localization of ore deposits in the Colorado mineral belt extend laterally into New Mexico. The shear zones in New Mexico coincide spatially with known epigenetic precious- and base-metal ore deposits; thus, the mineralized belts in the two states share a common inherited basement tectonic setting. Reactivation of the basement structures in Late Cretaceous-Eocene and Mid-Tertiary times provided zones of weakness for emplacement of magmas and conduits for ore-forming solutions. Ore deposits in the Colorado mineral belt are of both Late Cretaceous-Eocene and Mid-Tertiary age; those in New Mexico are predominantly Mid-Tertiary in age, but include Late Cretaceous porphyry-copper deposits in southwestern New Mexico. The mineralized belt in New Mexico, named the New Mexico structural zone, is 250-km wide. The northwest boundary is the Jemez subzone (or the approximately equivalent Globe belt), and the southeastern boundary was approximately marked by the Santa Rita belt. Three groups (subzones) of mineral deposits characterize the structural zone: (1) Mid-Tertiary porphyry molybdenite and alkaline-precious-metal deposits, in the northeast segment of the Jemez zone; (2) Mid-Tertiary epithermal precious-metal deposits in the Tijeras (intermediate) zone; and (3) Late Cretaceous porphyry-copper deposits in the Santa Rita zone. The structural zone was inferred to extend from New Mexico into adjacent Arizona. The structural zone provides favorable sites for exploration, particularly those parts of the Jemez subzone covered by Neogene volcanic and sedimentary rocks. ?? 2002 Published by Elsevier Science B.V.

Pathogenesis of Acute and Delayed Corneal Lesions after Ocular Exposure to Sulfur Mustard Vapor

DTIC Science & Technology

2012-01-01

mechanistic understanding and therapeutic development. Here we evaluate the histopathologic, biochemical and ultrastructural expressions of...were identified, including destabilization of the basal corneal epithelium , basement membrane zone abnormalities and stromal deformation. Clinical...pathology of MGK, in part resulting from persistent necrosis of the basal corneal epithelium and deterioration of the basement membrane. The findings

Listeria monocytogenes InlP interacts with afadin and facilitates basement membrane crossing.

PubMed

Faralla, Cristina; Bastounis, Effie E; Ortega, Fabian E; Light, Samuel H; Rizzuto, Gabrielle; Nocadello, Salvatorre; Anderson, Wayne F; Robbins, Jennifer R; Theriot, Julie A; Bakardjiev, Anna I

2018-05-30

During pregnancy, the placenta protects the fetus against the maternal immune response, as well as bacterial and viral pathogens. Bacterial pathogens that have evolved specific mechanisms of breaching this barrier, such as Listeria monocytogenes, present a unique opportunity for learning how the placenta carries out its protective function. We previously identified the L. monocytogenes protein Internalin P (InlP) as a secreted virulence factor critical for placental infection. Here, we show that InlP, but not the highly similar L. monocytogenes internalin Lmo2027, binds to human afadin (encoded by AF-6), a protein associated with cell-cell junctions. A crystal structure of InlP reveals several unique features, including an extended leucine-rich repeat (LRR) domain with a distinctive Ca2+-binding site. Despite afadin's involvement in the formation of cell-cell junctions, MDCK epithelial cells expressing InlP displayed a decrease in the magnitude of the traction stresses they could exert on deformable substrates, similar to the decrease in traction exhibited by AF-6 knock-out MDCK cells. L. monocytogenes ΔinlP mutants were deficient in their ability to form actin-rich protrusions from the basal face of polarized epithelial monolayers, a necessary step in the crossing of such monolayers (transcytosis). A similar phenotype was observed for bacteria expressing an internal in-frame deletion in inlP (inlP ΔLRR5) that specifically disrupts its interaction with afadin. However, afadin deletion in the host cells did not rescue the transcytosis defect. We conclude that secreted InlP targets cytosolic afadin to specifically promote L. monocytogenes transcytosis across the basal face of epithelial monolayers, which may contribute to the crossing of the basement membrane during placental infection.

Primary cellular meningeal defects cause neocortical dysplasia and dyslamination

PubMed Central

Hecht, Jonathan H.; Siegenthaler, Julie A.; Patterson, Katelin P.; Pleasure, Samuel J.

2010-01-01

Objective Cortical malformations are important causes of neurological morbidity, but in many cases their etiology is poorly understood. Mice with Foxc1 mutations have cellular defects in meningeal development. We use hypomorphic and null alleles of Foxc1 to study the effect of meningeal defects on neocortical organization. Methods Embryos with loss of Foxc1 activity were generated using the hypomorphic Foxc1hith allele and the null Foxc1lacZ allele. Immunohistologic analysis was used to assess cerebral basement membrane integrity, marginal zone heterotopia formation, neuronal overmigration, meningeal defects, and changes in basement membrane composition. Dysplasia severity was quantified using two measures. Results Cortical dysplasia resembling cobblestone cortex, with basement membrane breakdown and lamination defects, is seen in Foxc1 mutants. As Foxc1 activity was reduced, abnormalities in basement membrane integrity, heterotopia formation, neuronal overmigration, and meningeal development appeared earlier in gestation and were more severe. Surprisingly, the basement membrane appeared intact at early stages of development in the face of severe deficits in meningeal development. Prominent defects in basement membrane integrity appeared as development proceeded. Molecular analysis of basement membrane laminin subunits demonstrated that loss of the meninges led to changes in basement membrane composition. Interpretation Cortical dysplasia can be caused by cellular defects in the meninges. The meninges are not required for basement membrane establishment but are needed for remodeling as the brain expands. Specific changes in basement membrane composition may contribute to subsequent breakdown. Our study raises the possibility that primary meningeal defects may cortical dysplasia in some cases. PMID:20976766

COL4A3 founder mutations in Greek-Cypriot families with thin basement membrane nephropathy and focal segmental glomerulosclerosis dating from around 18th century.

PubMed

Voskarides, Konstantinos; Patsias, Charalampos; Pierides, Alkis; Deltas, Constantinos

2008-06-01

Mutations in the COL4A3/COL4A4 genes of type IV collagen account for about 40% of cases of thin basement membrane nephropathy, a condition that is estimated to affect 1% or more of the general population. We recently described 10 Cypriot families with familial hematuria and thin basement membrane nephropathy in the presence of focal segmental glomerulosclerosis, with founder mutations on COL4A3 gene. Seven of the families carried mutation G1334E on haplotype K, and another three carried mutation G871C on haplotype Ky. In this report we performed extension of the haplotypes with additional polymorphic markers, 12 for haplotype K and 22 for haplotype Ky, to estimate the linkage disequilibrium value between the mutation and flanking noncommon markers. Haplotype Ky extended to 13.71 Mb, but we did not attempt further analysis owing to the small number of chromosomes. Haplotype K extended to 3.83 Mb, thereby suggesting that it was a much older event compared to mutation G871C. Mutation G1334E was calculated to be about 5-10 generations old with a possible origin between 1693 and 1818 AD, during the Ottoman ruling of the island. Both mutations are clustered in specific geographic regions with apparently formerly isolated populations, although mutation G1334E has been detected elsewhere on the island. The identification of founder mutations in large families with microscopic hematuria greatly facilitates presymptomatic diagnosis and provides useful information on the history of the population, while it may also assist in association studies in search for disease modifier genes.

Necessary Conditions for Intraplate Seismic Zones in North America

NASA Astrophysics Data System (ADS)

Thomas, William A.; Powell, Christine A.

2017-12-01

The cause of intraplate seismic zones persists as an important scientific and societal question. Most intraplate earthquakes are concentrated in specific seismic zones along or adjacent to large-scale basement structures (e.g., rifts or sutures at ancient plate boundaries) within continental crust. The major intraplate seismic zones are limited to specific segments and are not distributed along the lengths of the ancient structures. We present a new hypothesis that major intraplate seismic zones are restricted to places where concentrated crustal deformation (CCD) is overprinted on large-scale basement structures. Examples where CCD affects the stability of specific parts of large-scale structures in response to present-day stress conditions include the most active seismic zones in central and eastern North America: Charlevoix, Eastern Tennessee, and New Madrid. Our hypothesis has important implications for the assessment of seismic hazards.

20. VIEW OF THE BASEMENT FLOOR PLAN. THE BASEMENT AREA ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. VIEW OF THE BASEMENT FLOOR PLAN. THE BASEMENT AREA INCLUDES A UTILITY ROOM, PROCESS WASTE STORAGE AND MAINTENANCE AREAS, AND THE ENTRANCE TO AN UNDERGROUND TUNNEL LEADING TO BUILDING 881. THE ORIGINAL DRAWING HAS BEEN ARCHIVED ON MICROFILM. THE DRAWING WAS REPRODUCED AT THE BEST QUALITY POSSIBLE. LETTERS AND NUMBERS IN THE CIRCLES INDICATE FOOTER AND/OR COLUMN LOCATIONS. - Rocky Flats Plant, Uranium Rolling & Forming Operations, Southeast section of plant, southeast quadrant of intersection of Central Avenue & Eighth Street, Golden, Jefferson County, CO

Depth-to-basement, sediment-thickness, and bathymetry data for the deep-sea basins offshore of Washington, Oregon, and California

USGS Publications Warehouse

Wong, Florence L.; Grim, Muriel S.

2015-01-01

Contours and derivative raster files of depth-to-basement, sediment-thickness, and bathymetry data for the area offshore of Washington, Oregon, and California are provided here as GIS-ready shapefiles and GeoTIFF files. The data were used to generate paper maps in 1992 and 1993 from 1984 surveys of the U.S. Exclusive Economic Zone by the U.S. Geological Survey for depth to basement and sediment thickness, and from older data for the bathymetry.

Basement membrane reconstruction in human skin equivalents is regulated by fibroblasts and/or exogenously activated keratinocytes.

PubMed

El Ghalbzouri, Abdoelwaheb; Jonkman, Marcel F; Dijkman, Remco; Ponec, Maria

2005-01-01

This study was undertaken to examine the role fibroblasts play in the formation of the basement membrane (BM) in human skin equivalents. For this purpose, keratinocytes were seeded on top of fibroblast-free or fibroblast-populated collagen matrix or de-epidermized dermis and cultured in the absence of serum and exogenous growth factors. The expression of various BM components was analyzed on the protein and mRNA level. Irrespective of the presence or absence of fibroblasts, keratin 14, hemidesmosomal proteins plectin, BP230 and BP180, and integrins alpha1beta1, alpha2beta1, alpha3beta1, and alpha6beta4 were expressed but laminin 1 was absent. Only in the presence of fibroblasts or of various growth factors, laminin 5 and laminin 10/11, nidogen, uncein, type IV and type VII collagen were decorating the dermal/epidermal junction. These findings indicate that the attachment of basal keratinocytes to the dermal matrix is most likely mediated by integrins alpha1beta1 and alpha2beta1, and not by laminins that bind to integrin alpha6beta4 and that the epithelial-mesenchymal cross-talk plays an important role in synthesis and deposition of various BM components.

Long-term outcome of anti-glomerular basement membrane antibody disease treated with immunoadsorption.

PubMed

Biesenbach, Peter; Kain, Renate; Derfler, Kurt; Perkmann, Thomas; Soleiman, Afschin; Benharkou, Alexandra; Druml, Wilfred; Rees, Andrew; Säemann, Marcus D

2014-01-01

Anti-glomerular basement membrane (GBM) antibody disease may lead to acute crescentic glomerulonephritis with poor renal prognosis. Current therapy favours plasma exchange (PE) for removal of pathogenic antibodies. Immunoadsorption (IAS) is superior to PE regarding efficiency of antibody-removal and safety. Apart from anecdotal data, there is no systemic analysis of the long-term effects of IAS on anti-GBM-disease and antibody kinetics. To examine the long-term effect of high-frequency IAS combined with standard immunosuppression on patient and renal survival in patients with anti-GBM-disease and to quantify antibody removal and kinetics through IAS. Retrospective review of patients treated with IAS for anti-GBM-antibody disease confirmed by biopsy and/or anti-GBM-antibodies. University Hospital of Vienna, Austria. 10 patients with anti-GBM-disease treated with IAS. Patient and renal survival, renal histology, anti-GBM-antibodies. Anti-GBM-antibodies were reduced by the first 9 IAS treatments (mean number of 23) to negative levels in all patients. Renal survival was 40% at diagnosis, 70% after the end of IAS, 63% after one year and 50% at the end of observation (mean 84 months, range 9 to 186). Dialysis dependency was successfully reversed in three of six patients. Patient survival was 90% at the end of observation. IAS efficiently eliminates anti-GBM-antibodies suggesting non-inferiority to PE with regard to renal and patient survival. Hence IAS should be considered as a valuable treatment option for anti-GBM-disease, especially in patients presenting with a high percentage of crescents and dialysis dependency due to an unusual high proportion of responders.

Viruses in the Oceanic Basement.

PubMed

Nigro, Olivia D; Jungbluth, Sean P; Lin, Huei-Ting; Hsieh, Chih-Chiang; Miranda, Jaclyn A; Schvarcz, Christopher R; Rappé, Michael S; Steward, Grieg F

2017-03-07

Microbial life has been detected well into the igneous crust of the seafloor (i.e., the oceanic basement), but there have been no reports confirming the presence of viruses in this habitat. To detect and characterize an ocean basement virome, geothermally heated fluid samples (ca. 60 to 65°C) were collected from 117 to 292 m deep into the ocean basement using seafloor observatories installed in two boreholes (Integrated Ocean Drilling Program [IODP] U1362A and U1362B) drilled in the eastern sediment-covered flank of the Juan de Fuca Ridge. Concentrations of virus-like particles in the fluid samples were on the order of 0.2 × 10 5 to 2 × 10 5  ml -1 ( n = 8), higher than prokaryote-like cells in the same samples by a factor of 9 on average (range, 1.5 to 27). Electron microscopy revealed diverse viral morphotypes similar to those of viruses known to infect bacteria and thermophilic archaea. An analysis of virus-like sequences in basement microbial metagenomes suggests that those from archaeon-infecting viruses were the most common (63 to 80%). Complete genomes of a putative archaeon-infecting virus and a prophage within an archaeal scaffold were identified among the assembled sequences, and sequence analysis suggests that they represent lineages divergent from known thermophilic viruses. Of the clustered regularly interspaced short palindromic repeat (CRISPR)-containing scaffolds in the metagenomes for which a taxonomy could be inferred (163 out of 737), 51 to 55% appeared to be archaeal and 45 to 49% appeared to be bacterial. These results imply that the warmed, highly altered fluids in deeply buried ocean basement harbor a distinct assemblage of novel viruses, including many that infect archaea, and that these viruses are active participants in the ecology of the basement microbiome. IMPORTANCE The hydrothermally active ocean basement is voluminous and likely provided conditions critical to the origins of life, but the microbiology of this vast habitat is not

Architectural and Biochemical Expressions of Mustard Gas Keratopathy: Preclinical Indicators and Pathogenic Mechanisms

DTIC Science & Technology

2012-08-10

development. Here we evaluate the histopathologic, biochemical and ultrastructural expressions of pathogenesis of the chronic SM injury over eight weeks...the basal corneal epithelium , basement membrane zone abnormalities and stromal deformation. Clinical sequelae of MGK appeared abruptly three weeks after...persistent necrosis of the basal corneal epithelium and deterioration of the basement membrane. The findings also provide a potential explanation as to why

Matrigel basement membrane matrix induces eccrine sweat gland cells to reconstitute sweat gland-like structures in nude mice.

PubMed

Li, Haihong; Chen, Lu; Zeng, Shaopeng; Li, Xuexue; Zhang, Xiang; Lin, Changmin; Zhang, Mingjun; Xie, Sitian; He, Yunpu; Shu, Shenyou; Yang, Lvjun; Tang, Shijie; Fu, Xiaobing

2015-03-01

Severe burn results in irreversible damage to eccrine sweat glands, for which no effective treatment is available. Interaction between the extracellular matrix and epithelial cells is critical for proper three-dimensional organization and function of the epithelium. Matrigel-embedded eccrine sweat gland cells were subcutaneously implanted into the inguinal regions of nude mice. Two weeks later, the Matrigel plugs were removed and evaluated for series of detection items. Sweat gland cells developed into sweat gland-like structures in the Matrigel plugs based on: (1) de novo formation of tubular-like structures with one or more hollow lumens, (2) expression of epithelial and sweat gland markers (pancytokeratin, CK5/7/14/19, α-SMA and CEA), (3) basement membrane formation, (4) myoepithelial cells presenting in and encompassing the tubular-like structures, (5) cellular polarization, evident by the expression of tight junction proteins (claudin-1 and ZO-2), anchoring junctions (desmoglein-1 and -2 and E-cadherin) and CEA in the luminal membrane, (6) expression of proteins related to sweat secretion and absorption (Na(+)-K(+)-ATPase α/β, Na(+)-K(+)-2Cl-cotranspoter 1, Na(+)/H(+) exchanger 1, aquaporin-5, epithelial sodium channel, cystic fibrosis transmembrane conductance regulator, potassium channel and vacuolar-type H+-ATPase), and (7) about 20% of the tubular-like structures are de novo coils and 80% are de novo ducts. This study provides not only an excellent model to study eccrine sweat gland development, cytodifferentiation and reconstitution, but also an in vivo model for regeneration of eccrine sweat glands. Copyright © 2015 Elsevier Inc. All rights reserved.

Gondwanan/peri-Gondwanan origin for the Uchee terrane, Alabama and georgia: Carolina zone or Suwannee terrane(?) and its suture with Grenvillian basement of the Pine Mountain window

USGS Publications Warehouse

Steltenpohl, M.G.; Mueller, P.M.; Heatherington, A.L.; Hanley, T.B.; Wooden, J.L.

2008-01-01

The poorly known, suspect, Uchee terrane occupies a critical tectonic position with regard to how and when peri-Gondwanan (Carolina) and Gondwanan (Suwannee) terranes were sutured to Laurentia. It lies sandwiched between Laurentian(?) continental basement exposed in the Pine Mountain window and adjacent buried Gondwanan crust of the Suwannee terrane. The Uchee terrane has been proposed as both a septum of Piedmont rocks that once was continuous across the erosionally breached Pine Mountain window or part of the Carolina zone. To help resolve this issue, we conducted U-Pb (SHRIMP-RG) (sensitive high-resolution ion microprobe-reverse geometry) zircon studies and whole-rock isotopic analyses of principal metasedimentary and metaplutonic units. U-Pb ages for zircons from the Phenix City Gneiss suggest igneous crystallization at ca. 620 Ma, inheritance ca. 1000 to ca. 1700 Ma, and a ca. 300 Ma (Alleghanian) overprint recorded by zircon rims. Zircons from the metasedimentary/metavolcaniclastic Moffits Mill Schist yield bimodal dates at ca. 620 and 640 Ma. The 620 to 640 Ma dates make these rocks age-equivalent to the oldest parts of the Carolina slate belt (Virgilina and Savannah River) and strongly suggest a Gondwanan (Pan-African and/or Trans-Brasiliano) origin for the Uchee terrane. Alternatively, the Uchee terrane may be correlative with metamorphic basement of the Suwannee terrane. The ca. 300 Ma overgrowths on zircons are compatible with previously reported 295 to 288 Ma 40Ar/39Ar hornblende dates on Uchee terrane rocks, which were interpreted to indicate deep tectonic burial of the Uchee terrane contemporaneous with the Alleghanian orogeny recorded in the foreland. Temperature-time paths for the Uchee terrane are similar to that of the Pine Mountain terrane, indicating a minimum age of ca. 295 Ma for docking. In terms of tectono-metamorphic history of the Uchee terrane, it is important to note that no evidence for intermediate "Appalachian" dates (e.g, Acadian or

Thick-skinned tectonics in a Late Cretaceous-Neogene intracontinental belt (High Atlas Mountains, Morocco): The flat-ramp fault control on basement shortening and cover folding

NASA Astrophysics Data System (ADS)

Fekkak, A.; Ouanaimi, H.; Michard, A.; Soulaimani, A.; Ettachfini, E. M.; Berrada, I.; El Arabi, H.; Lagnaoui, A.; Saddiqi, O.

2018-04-01

Most of the structural studies of the intracontinental High Atlas belt of Morocco have dealt with the central part of the belt, whose basement does not crop out. Here we study the Alpine deformation of the North Subatlas Zone, which is the part of the Western High Atlas (WHA) Paleozoic Massif that involves both Paleozoic basement units and remnants of their Mesozoic-Cenozoic cover formations. Our aim is to better constrain the geometry and kinematics of the basement faults during the Alpine shortening. Based on detail mapping, satellite imagery and field observations, we describe an array of sub-equatorial, transverse and oblique faults between the WHA Axial Zone and the Haouz Neogene basin. They define a mosaic of basement blocks pushed upon one another and upon the Haouz basement along the North Atlas Fault (NAF). The Axial Zone makes up the hanging-wall of the Adassil-Medinet Fault (AMF) south of this mosaic. The faults generally presents flat-ramp-flat geometry linked to the activation of multiple décollement levels, either within the basement where its foliation is subhorizontal or within favourable cover formations (Jurassic evaporites, Lower Cretaceous silty red beds, Upper Cretaceous evaporitic marls, Neogene basal argillites). The occurrence of the North Atlas detachment (NAD) allowed folded pop-up units to develop in front of the propagating NAF. Shortening began as early as the Campanian-Maastrichtian along the AMF. The direction of the maximum horizontal stress rotated from NNE-SSW to NNW-SSE from the Maastrichtian-Paleocene to the Neogene. The amount of shortening reaches 20% in the Azegour transect. This compares with the shortening amount published for the central-eastern High Atlas, suggesting that similar structures characterize the Paleozoic basement all along the belt. The WHA thick-skinned tectonics evokes that of the frontal Sevier belt and of the external Western Alps, although with a much minor pre-inversion burial.

Permeation of macromolecules into the renal glomerular basement membrane and capture by the tubules

PubMed Central

Lawrence, Marlon G.; Altenburg, Michael K.; Sanford, Ryan; Willett, Julian D.; Bleasdale, Benjamin; Ballou, Byron; Wilder, Jennifer; Li, Feng; Miner, Jeffrey H.; Berg, Ulla B.; Smithies, Oliver

2017-01-01

How the kidney prevents urinary excretion of plasma proteins continues to be debated. Here, using unfixed whole-mount mouse kidneys, we show that fluorescent-tagged proteins and neutral dextrans permeate into the glomerular basement membrane (GBM), in general agreement with Ogston's 1958 equation describing how permeation into gels is related to molecular size. Electron-microscopic analyses of kidneys fixed seconds to hours after injecting gold-tagged albumin, negatively charged gold nanoparticles, and stable oligoclusters of gold nanoparticles show that permeation into the lamina densa of the GBM is size-sensitive. Nanoparticles comparable in size with IgG dimers do not permeate into it. IgG monomer-sized particles permeate to some extent. Albumin-sized particles permeate extensively into the lamina densa. Particles traversing the lamina densa tend to accumulate upstream of the podocyte glycocalyx that spans the slit, but none are observed upstream of the slit diaphragm. At low concentrations, ovalbumin-sized nanoparticles reach the primary filtrate, are captured by proximal tubule cells, and are endocytosed. At higher concentrations, tubular capture is saturated, and they reach the urine. In mouse models of Pierson’s or Alport’s proteinuric syndromes resulting from defects in GBM structural proteins (laminin β2 or collagen α3 IV), the GBM is irregularly swollen, the lamina densa is absent, and permeation is increased. Our observations indicate that size-dependent permeation into the lamina densa of the GBM and the podocyte glycocalyx, together with saturable tubular capture, determines which macromolecules reach the urine without the need to invoke direct size selection by the slit diaphragm. PMID:28246329

WY14,643, a PPARalpha ligand, attenuates expression of anti-glomerular basement membrane disease.

PubMed

Archer, D C; Frkanec, J T; Cromwell, J; Clopton, P; Cunard, R

2007-11-01

Peroxisome proliferator-activated receptor alpha (PPARalpha) ligands are medications used to treat hyperlipidaemia and atherosclerosis. Increasing evidence suggests that these agents are immunosuppressive. In the following studies we demonstrate that WY14,643, a PPARalpha ligand, attenuates expression of anti-glomerular basement membrane disease (AGBMD). C57BL/6 mice were fed 0.05% WY14,643 or control food and immunized with the non-collagenous domain of the alpha3 chain of Type IV collagen [alpha3(IV) NC1] in complete Freund's adjuvant (CFA). WY14,643 reduced proteinuria and greatly improved glomerular and tubulo-interstitial lesions. However, the PPARalpha ligand did not alter the extent of IgG-binding to the GBM. Immunohistochemical studies revealed that the prominent tubulo-interstitial infiltrates in the control-fed mice consisted predominately of F4/80(+) macrophages and WY14,643-feeding decreased significantly the number of renal macrophages. The synthetic PPARalpha ligand also reduced significantly expression of the chemokine, monocyte chemoattractant protein (MCP)-1/CCL2. Sera from mice immunized with AGBMD were also evaluated for antigen-specific IgGs. There was a significant increase in the IgG1 : IgG2c ratio and a decline in the intrarenal and splenocyte interferon (IFN)-gamma mRNA expression in the WY14,643-fed mice, suggesting that the PPARalpha ligand could skew the immune response to a less inflammatory T helper 2-type of response. These studies suggest that PPARalpha ligands may be a novel treatment for inflammatory renal disease.

WY14,643, a PPARα ligand, attenuates expression of anti-glomerular basement membrane disease

PubMed Central

Archer, D C; Frkanec, J T; Cromwell, J; Clopton, P; Cunard, R

2007-01-01

Peroxisome proliferator-activated receptor alpha (PPARα) ligands are medications used to treat hyperlipidaemia and atherosclerosis. Increasing evidence suggests that these agents are immunosuppressive. In the following studies we demonstrate that WY14,643, a PPARα ligand, attenuates expression of anti-glomerular basement membrane disease (AGBMD). C57BL/6 mice were fed 0·05% WY14,643 or control food and immunized with the non-collagenous domain of the α3 chain of Type IV collagen [α3(IV) NC1] in complete Freund's adjuvant (CFA). WY14,643 reduced proteinuria and greatly improved glomerular and tubulo-interstitial lesions. However, the PPARα ligand did not alter the extent of IgG-binding to the GBM. Immunohistochemical studies revealed that the prominent tubulo-interstitial infiltrates in the control-fed mice consisted predominately of F4/80+ macrophages and WY14,643-feeding decreased significantly the number of renal macrophages. The synthetic PPARα ligand also reduced significantly expression of the chemokine, monocyte chemoattractant protein (MCP)-1/CCL2. Sera from mice immunized with AGBMD were also evaluated for antigen-specific IgGs. There was a significant increase in the IgG1 : IgG2c ratio and a decline in the intrarenal and splenocyte interferon (IFN)-γ mRNA expression in the WY14,643-fed mice, suggesting that the PPARα ligand could skew the immune response to a less inflammatory T helper 2-type of response. These studies suggest that PPARα ligands may be a novel treatment for inflammatory renal disease. PMID:17888025

Basement structure based on gravity anomaly in the northern Noto peninsula, Central Japan

NASA Astrophysics Data System (ADS)

Mizubayashi, T.; Sawada, A.; Hamada, M.; Hiramatsu, Y.; Honda, R.

2012-12-01

(density is 2670kg/m3), Neocene volcanic rock (density is 2400kg/m3, or 2550kg/m3), Neocene sedimentary rock (density is 2200kg/m3), and Quaternary sedimentary rock (density is 1800kg/m3, or 1500kg/m3) (Honda et al., 2008). To compare our basement model to the geological block structures, we focus on a transition zone of the basement depth. We recognize that two of three geological block boundaries correspond to the transition zones. These boundaries also correspond to the boundary of active fault segments on the seafloor. Therefore, based on the relationship between the source fault of the 2007 Noto Hanto earthquake and the geological block, we suggest that the movement of those geological blocks is possibly controlled by the corresponding active fault segments. However, we find that the other block boundary doesn't correspond to the transition zone.

Crosslinked basement membrane-based coatings enhance glucose sensor function and continuous glucose monitoring in vivo.

PubMed

Klueh, Ulrike; Ludzinska, Izabela; Czajkowski, Caroline; Qiao, Yi; Kreutzer, Donald L

2018-01-01

Overcoming sensor-induced tissue reactions is an essential element of achieving successful continuous glucose monitoring (CGM) in the management of diabetes, particularly when used in closed loop technology. Recently, we demonstrated that basement membrane (BM)-based glucose sensor coatings significantly reduced tissue reactions at sites of device implantation. However, the biocompatible BM-based biohydrogel sensor coating rapidly degraded over a less than a 3-week period, which effectively eliminated the protective sensor coating. In an effort to increase the stability and effectiveness of the BM coating, we evaluated the impact of crosslinking BM utilizing glutaraldehyde as a crosslinking agent, designated as X-Cultrex. Sensor performance (nonrecalibrated) was evaluated for the impact of these X-Cultrex coatings in vitro and in vivo. Sensor performance was assessed over a 28-day time period in a murine CGM model and expressed as mean absolute relative difference (MARD) values. Tissue reactivity of Cultrex-coated, X-Cultrex-coated, and uncoated glucose sensors was evaluated over a 28-day time period in vivo using standard histological techniques. These studies demonstrated that X-Cultrex-based sensor coatings had no effect on glucose sensor function in vitro. In vivo, glucose sensor performance was significantly enhanced following X-Cultrex coating throughout the 28-day study. Histological evaluations of X-Cultrex-treated sensors demonstrated significantly less tissue reactivity when compared to uncoated sensors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 7-16, 2018. © 2017 Wiley Periodicals, Inc.

A large-sized bubbling appearance of the glomerular basement membrane in a patient with pulmonary limited AL amyloidosis and a past history of lupus nephritis.

PubMed

Suga, Norihiro; Miura, Naoto; Uemura, Yuko; Nakamura, Toshinobu; Morita, Hiroyuki; Banno, Shogo; Imai, Hirokazu

2011-12-01

We report an unusual pathological finding, a large-sized bubbling appearance of the glomerular basement membrane (GBM), in a patient with pulmonary limited AL amyloidosis and a past history of lupus nephritis. The first renal biopsy specimen from 10 years ago, when systemic lupus erythematosus was diagnosed, demonstrated mild mesangial proliferation and subepithelial deposits (WHO classification: III + V). Light microscopy of the current biopsy using periodic acid methenamine silver (PAMS) stain demonstrated a large-sized bubbling appearance of the GBM; however, very weak immunoglobulin and complement deposition was observed in immunofluorescence studies. Routine electron microscopy demonstrated partial subendothelial expansion with electron-lucent materials, but no electron-dense deposits or amyloid fibrils. Electron microscopy with PAMS stain revealed electron-lucent endothelial scalloping, including some cellular components and microspheres in the GBM; however, it is not clear if these materials are derived from endothelial cells. One possibility is that these unique findings represent a recovery phase of lupus membranous nephritis; another is that these findings correspond to a new disease entity.

Phosphatidylinositol-4,5-Bisphosphate-Rich Plasma Membrane Patches Organize Active Zones of Endocytosis and Ruffling in Cultured Adipocytes

PubMed Central

Huang, Shaohui; Lifshitz, Larry; Patki-Kamath, Varsha; Tuft, Richard; Fogarty, Kevin; Czech, Michael P.

2004-01-01

A major regulator of endocytosis and cortical F-actin is thought to be phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] present in plasma membranes. Here we report that in 3T3-L1 adipocytes, clathrin-coated membrane retrieval and dense concentrations of polymerized actin occur in restricted zones of high endocytic activity. Ultrafast-acquisition and superresolution deconvolution microscopy of cultured adipocytes expressing an enhanced green fluorescent protein- or enhanced cyan fluorescent protein (ECFP)-tagged phospholipase Cδ1 (PLCδ1) pleckstrin homology (PH) domain reveals that these zones spatially coincide with large-scale PtdIns(4,5)P2-rich plasma membrane patches (PRMPs). PRMPs exhibit lateral dimensions exceeding several micrometers, are relatively stationary, and display extensive local membrane folding that concentrates PtdIns(4,5)P2 in three-dimensional space. In addition, a higher concentration of PtdIns(4,5)P2 in the membranes of PRMPs than in other regions of the plasma membrane can be detected by quantitative fluorescence microscopy. Vesicular structures containing both clathrin heavy chains and PtdIns(4,5)P2 are revealed immediately beneath PRMPs, as is dense F actin. Blockade of PtdIns(4,5)P2 function in PRMPs by high expression of the ECFP-tagged PLCδ1 PH domain inhibits transferrin endocytosis and reduces the abundance of cortical F-actin. Membrane ruffles induced by the expression of unconventional myosin 1c were also found to localize at PRMPs. These results are consistent with the hypothesis that PRMPs organize active PtdIns(4,5)P2 signaling zones in the adipocyte plasma membrane that in turn control regulators of endocytosis, actin dynamics, and membrane ruffling. PMID:15456883

Impact of climate changes during the last 5 million years on groundwater in basement aquifers.

PubMed

Aquilina, Luc; Vergnaud-Ayraud, Virginie; Les Landes, Antoine Armandine; Pauwels, Hélène; Davy, Philippe; Pételet-Giraud, Emmanuelle; Labasque, Thierry; Roques, Clément; Chatton, Eliot; Bour, Olivier; Ben Maamar, Sarah; Dufresne, Alexis; Khaska, Mahmoud; Le Gal La Salle, Corinne; Barbecot, Florent

2015-09-22

Climate change is thought to have major effects on groundwater resources. There is however a limited knowledge of the impacts of past climate changes such as warm or glacial periods on groundwater although marine or glacial fluids may have circulated in basements during these periods. Geochemical investigations of groundwater at shallow depth (80-400 m) in the Armorican basement (western France) revealed three major phases of evolution: (1) Mio-Pliocene transgressions led to marine water introduction in the whole rock porosity through density and then diffusion processes, (2) intensive and rapid recharge after the glacial maximum down to several hundred meters depths, (3) a present-day regime of groundwater circulation limited to shallow depth. This work identifies important constraints regarding the mechanisms responsible for both marine and glacial fluid migrations and their preservation within a basement. It defines the first clear time scales of these processes and thus provides a unique case for understanding the effects of climate changes on hydrogeology in basements. It reveals that glacial water is supplied in significant amounts to deep aquifers even in permafrosted zones. It also emphasizes the vulnerability of modern groundwater hydrosystems to climate change as groundwater active aquifers is restricted to shallow depths.

Antiproteinuric effect of pirfenidone in a rat model of anti-glomerular basement membrane glomerulonephritis.

PubMed

Takakura, Koji; Mizukami, Kazuhiko; Mitori, Hikaru; Noto, Takahisa; Tomura, Yuichi

2014-08-15

While pirfenidone has been established as an effective anti-fibrosis remedy, whether or not its antifibrotic effect contributes to a reduction of proteinuria remains unclear. We investigated the renoprotective properties of pirfenidone in an anti-glomerular basement membrane (GBM) glomerulonephritis model both prophylactically and therapeutically to determine its profile against proteinuria. In the prophylactic regimen, pirfenidone was treated immediately after anti-serum injection. We observed a significant reduction in the progression of proteinuria (P<0.05) and decline in renal function (P<0.01) and also noted histological improvement in renal injury. These effects appeared to be due to the maintained expression of nephrin and podocin on podocytes as well as the reduced expression of profibrotic factors like transforming growth factor-β (TGF-β). The expression of nephrin mRNA was strongly negatively correlated with the amount of urinary protein excretion (R=-0.84, P<0.001), implicating podocyte damage in the outcome of proteinuria (R(2)=0.70). These results suggest that preservation of podocytes with the pirfenidone treatment may have resulted in the decrease of proteinuria. In contrast, when the therapeutic regimen was initiated 2 weeks after nephritis induction, pirfenidone had little effect on the progression of proteinuria, although the decline of renal function and fibrosis were suppressed. Taken together, present findings suggested that pirfenidone prevented the progression of proteinuria only when administered prophylactically but was still able to ameliorate the decline of renal function independent of proteinuria. In conclusion, pirfenidone as a prophylactic regimen reduces proteinuria in anti-GBM nephritis via preservation of podocytes with markedly reduced efficacy when administered as a therapeutic regimen. Copyright © 2014 Elsevier B.V. All rights reserved.

Modulation of interferon-induced genes by lipoxin analogue in anti-glomerular basement membrane nephritis.

PubMed

Ohse, Takamoto; Ota, Tatsuru; Kieran, Niamh; Godson, Catherine; Yamada, Koei; Tanaka, Tetsuhiro; Fujita, Toshiro; Nangaku, Masaomi

2004-04-01

Immune complex deposition is associated with the accumulation of neutrophils, which play an important role in the various immune-mediated diseases. A novel anti-inflammatory agent, the lipoxin A (LXA) analogue (15-epi-16-(FPhO)-LXA-Me)), a stable synthetic analogue of aspirin-triggered 15-epi-lipoxin A4 (ATLa), was used in experimental anti-glomerular basement membrane (GBM) antibody nephritis in mice. ATLa was administered before the induction of the disease, and 2 h later, the animals were killed. ATLa reduced the infiltrating neutrophils and nitrotyrosine staining in glomeruli. Subsequent changes of gene expression in the early phase were evaluated, and 5674 genes were present under the basal conditions in kidneys from normal mice; 54 upregulated genes and 25 downregulated genes were detected in anti-GBM nephritis. Eighteen of these upregulated genes were those induced by IFN-gamma. Real-time quantitative PCR analysis confirmed the results of the microarrays. To investigate a role of IFN-gamma in neutrophil infiltration, anti-GBM nephritis was induced in IFN-gamma knockout mice. The number of infiltrating neutrophils in these mice did not differ from those in wild-type mice. Also examined were CD11b expression on neutrophils from mice treated with ATLa by flow cytometry, but suppression of this adhesion molecule was not observed. Neutrophil infiltration was successfully inhibited by ATLa in the early phase of murine anti-GBM nephritis. Microarray analysis detected the change of mRNA expression in anti-GBM nephritis and demonstrated amelioration of various genes by ATLa, which may provide a clue to the development of novel therapeutic approaches in immune renal injury.

Did the Basement-Involved Main Caucasus Thrust Form during the Cenozoic Arabia-Eurasia Collision?

NASA Astrophysics Data System (ADS)

Vasey, D. A.; Cowgill, E.; Niemi, N. A.; Godoladze, T.; Javakhishvili, Z.; Skhirtladze, I.; Boichenko, G.

2017-12-01

The Greater Caucasus Mountains lie between the Black and Caspian Seas at the northern margin of the active Arabia-Eurasia collision zone. The north-dipping Main Caucasus Thrust (MCT) is commonly assumed to be a first-order structure within the range that places Paleozoic crystalline basement to the north over metasedimentary cover of inferred Mesozoic age to the south. Although most workers assume this juxtaposition occurred during Cenozoic growth of the range, the timing of ductile (quartz-plastic) shearing along the MCT remains to be established. Here, we present data to discriminate between two competing models of quartz-plastic deformation along the proposed MCT location at the basement-cover contact. In the first model, quartz-plastic deformation occurred during the Cenozoic Arabia-Eurasia collision, whereas in the second, this deformation took place during an older orogenic event, such as the Paleozoic Variscan orogeny. To test these models, we are combining field observations, microstructural investigations, and thermochronologic analyses on two 10 km-long traverses in the Republic of Georgia, separated by 200 km along strike, across the MCT. Our fieldwork and microstructural analyses along the basement-cover contact document north-dipping zones of high strain that are 100 m thick and show quartz-plastic deformation, top-to-the-south shear sense, and greenschist-facies metamorphism. Zircon (U-Th)/He (ZHe) analyses along the eastern traverse near Stepantsminda yielded latest Miocene ( 7-8 Ma) ages in both the MCT shear zone and 150 m structurally above the MCT. In contrast, along the western traverse near Nakra, a sample 300 m structurally below the MCT yielded an early Oligocene ( 31 Ma) ZHe age. These data require Cenozoic exhumation from temperatures >180° and are compatible with recent ductile shear along the MCT. However, results from biotite and muscovite 40Ar/39Ar analyses in progress are needed to confirm this hypothesis. The MCT appears to have

Expression of matrix metalloproteinase genes during basement membrane degradation in the metamorphosis of Bombyx mori.

PubMed

Kawasaki, Hideki; Manickam, Asaithambi; Shahin, Rima; Ote, Manabu; Iwanaga, Masashi

2018-01-05

The present study was conducted to clarify the involvement of the basement membrane (BM) in insect metamorphosis through analysis of the expression profile of two types of metalloproteinase (MMP and ADAMTS) genes in several organs, their ecdysone involvement, and the histological change of BM. BM was observed around wing sac and in the wing cavity and around fat bodies at the W0 stage but disappeared after the W3 stage, and wing discs evaginated and fat body cells scattered after the W3 stage. The disappearance of the BM of midgut and silk glands was not observed after the W3 stage, but degenerated epithelium cells in the midgut and shrunken cells in the silk gland were observed after the W3 stage. BmMMP1 showed a peak at P0 in the wing discs, fat bodies, midgut, and silk gland. BmMMP2 showed a broad peak around pupation in the wing discs, fat bodies, midgut, and silk gland. BmADAMTS-1 showed enhanced expression at W2 in the wing discs, fat bodies, midgut, and hemocyte, while BmADAMTS-L showed enhanced expression at W3 in the fat bodies, midgut, silk gland, and hemocyte. After pupation, they showed a different expression in different organs. All of four genes were induced by 20-hydroxyecdysone in wing discs in vitro. The present results suggested the involvement of MMPs and ADAMTS in the BM digestion and the morphogenesis of organs during Bombyx metamorphosis. Copyright © 2017 Elsevier B.V. All rights reserved.

The hyaloid vasculature facilitates basement membrane breakdown during choroid fissure closure in the zebrafish eye.

PubMed

James, Andrea; Lee, Chanjae; Williams, Andre M; Angileri, Krista; Lathrop, Kira L; Gross, Jeffrey M

2016-11-15

A critical aspect of vertebrate eye development is closure of the choroid fissure (CF). Defects in CF closure result in colobomas, which are a significant cause of childhood blindness worldwide. Despite the growing number of mutated loci associated with colobomas, we have a limited understanding of the cell biological underpinnings of CF closure. Here, we utilize the zebrafish embryo to identify key phases of CF closure and regulators of the process. Utilizing Laminin-111 as a marker for the basement membrane (BM) lining the CF, we determine the spatial and temporal patterns of BM breakdown in the CF, a prerequisite for CF closure. Similarly, utilizing a combination of in vivo time-lapse imaging, β-catenin immunohistochemistry and F-actin staining, we determine that tissue fusion, which serves to close the fissure, follows BM breakdown closely. Periocular mesenchyme (POM)-derived endothelial cells, which migrate through the CF to give rise to the hyaloid vasculature, possess distinct actin foci that correlate with regions of BM breakdown. Disruption of talin1, which encodes a regulator of the actin cytoskeleton, results in colobomas and these correlate with structural defects in the hyaloid vasculature and defects in BM breakdown. cloche mutants, which entirely lack a hyaloid vasculature, also possess defects in BM breakdown in the CF. Taken together, these data support a model in which the hyaloid vasculature and/or the POM-derived endothelial cells that give rise to the hyaloid vasculature contribute to BM breakdown during CF closure. Copyright © 2016 Elsevier Inc. All rights reserved.

ETR BASEMENT, TRA642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID33G101 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

ETR BASEMENT, TRA-642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID-33-G-101 AND ID-33-G-102) WITH TANK AND SODIUM-RELATED APPARATUS. CAMERA STANDS BEFORE ROLL-UP DOOR SHOWN IN PHOTO ID-33-G-101. INL NEGATIVE NO. HD24-3-3. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

Application of high resolution aeromagnetic data for basement topography mapping of Siluko and environs, southwestern Nigeria

NASA Astrophysics Data System (ADS)

Osinowo, Olawale O.; Akanji, Adesoji O.; Olayinka, Abel I.

2014-11-01

The discovery of hydrocarbon in commercial quantity in the Niger Delta, southern Nigeria, has since the early fifties shifted the attention of exploration/active geological studies from the Dahomey basin and the adjacent basement terrain in south-western Nigeria towards the south and this has left some gaps in information required for the discovery and exploitation of the economic potential of the region. This study mapped the Siluko transition zone in south-western Nigeria in terms of structures, geometry and basement topography with the object of providing requisite geological information that will engender interest in the exploration and exploitation of the numerous economic potentials of south-western part of Nigeria. Acquired high resolution aeromagnetic data were filtered, processed and enhanced, the resultant data were subjected to qualitative and quantitative magnetic interpretation, depth weighting analyses and modelling to generate the subsurface basement topography across the study area. The obtained results indicate regions of high and low magnetic anomalies with residual magnetic intensity values ranging from -100.8 nT to 100.9 nT. Euler Deconvolution indicates generally undulating basement topography with depth range of 125-1812 m. The basement relief is generally gentle and flat lying within the basement terrain with depth ranging from 125 to 500 m. However the sedimentary terrain is undulating and generally steeps south, down the basin with depth range of 300-1812 m. A basement topography model of the magnetic data constrained by Euler solutions correlate positively with the geology of the study area and indicates a generally increasing sedimentary deposits' thickness southward toward the western part of Dahomey basin. The revealed basement topography and structures as well as the delineated direction of continuous increase in thickness of sedimentary deposit provide insight to the controlling factor responsible for tar sand deposit and bitumen

Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.

PubMed

Funahashi, Junichiro; Tanaka, Hiromitsu; Hirano, Tomoo

2018-01-01

Fast repetitive synaptic transmission depends on efficient exocytosis and retrieval of synaptic vesicles around a presynaptic active zone. However, the functional organization of an active zone and regulatory mechanisms of exocytosis, endocytosis and reconstruction of release-competent synaptic vesicles have not been fully elucidated. By developing a novel visualization method, we attempted to identify the location of exocytosis of a single synaptic vesicle within an active zone and examined movement of synaptic vesicle protein synaptophysin (Syp) after exocytosis. Using cultured hippocampal neurons, we induced formation of active-zone-like membranes (AZLMs) directly adjacent and parallel to a glass surface coated with neuroligin, and imaged Syp fused to super-ecliptic pHluorin (Syp-SEP) after its translocation to the plasma membrane from a synaptic vesicle using total internal reflection fluorescence microscopy (TIRFM). An AZLM showed characteristic molecular and functional properties of a presynaptic active zone. It contained active zone proteins, cytomatrix at the active zone-associated structural protein (CAST), Bassoon, Piccolo, Munc13 and RIM, and showed an increase in intracellular Ca 2+ concentration upon electrical stimulation. In addition, single-pulse stimulation sometimes induced a transient increase of Syp-SEP signal followed by lateral spread in an AZLM, which was considered to reflect an exocytosis event of a single synaptic vesicle. The diffusion coefficient of Syp-SEP on the presynaptic plasma membrane after the membrane fusion was estimated to be 0.17-0.19 μm 2 /s, suggesting that Syp-SEP diffused without significant obstruction. Synchronous exocytosis just after the electrical stimulation tended to occur at multiple restricted sites within an AZLM, whereas locations of asynchronous release occurring later after the stimulation tended to be more scattered.

Occupant radon exposure in houses with basements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Franklin, E.M.; Fuoss, S.

1995-12-31

This study compares basement and main-level radon exposure based on bi-level week-long radon measurements, occupancy and activity data collected in normal use during heating and non-heating seasons in a geographically-stratified random sample of about 600 Minnesota homes, in response to critiques of radon measurement protocol. Basement radon (RN1) (M=4.5, SD=4.5) and main level (Rn2)(M=2.9, SD=3.4) correlation was 0.8 (p=.00), including seasonal variation. In a 101-house subsample where Rn1 >=4.0 pCi/L and Rn2 <=3.9 pCi/L, maximum household exposure in basements was 1162 pCiHrs (M=120, Sd=207), main-level 2486 pCiHrs (M-434, SD=421). In same households, persons with most basement-time maxed 100 hrs (M=13,SD=23),more » persons with most main-level time maxed 160 hrs (M=79, SD=39). Basement activities show two patterns, (1) member used it for personal domain, e.g. sleeping, and (2) household used it for general activities, e.g. TV or children`s play. Basement occupancy justifies measurement of radon in the lowest livable housing level.« less

Geophysical-geological studies of possible extensions of the New Madrid Fault Zone. Annual report, 1982. Vol. 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hinze, W.J.; Braile, L.W.; Keller, G.R.

1983-05-01

An integrated geophysical/geologic program is being conducted to evaluate the rift complex hypothesis as an explanation for the earthquake activity in the New Madrid Seismic Zone and its extensions, to refine our knowledge of the rift complex, and to investigate the possible northern extensions of the New Madrid Fault Zone, especially its possible connection to the Anna, Ohio seismogenic region. Drillhole basement lithologies are being investigated to aid in tectonic analysis and geophysical interpretation, particularly in the Anna, Ohio area. Gravity and magnetic modeling combined with limited seismic reflection studies in southwest Indiana are interpreted as confirming speculation that anmore » arm of the New Madrid Rift Complex extends northeasterly into Indiana. The geologic and geophysical evidence confirm that the basement lithology in the Anna, Ohio area is highly variable reflecting a complex geologic history. The data indicate that as many as three major Late Precambrian tectonic features intersect within the basement of the Anna area suggesting that the seismicity may be related to basement zones of weakness.« less

Influence of basements, garages, and common hallways on indoor residential volatile organic compound concentrations

NASA Astrophysics Data System (ADS)

Dodson, Robin E.; Levy, Jonathan I.; Spengler, John D.; Shine, James P.; Bennett, Deborah H.

Concentrations of many volatile organic compounds (VOCs) are often higher inside residences than outdoors as a result of sources or activities within the residences. These sources can be located directly in the living space of the home or in areas associated with the home such as an attached garage, basement, or common apartment hallway. To characterize the contributions from these areas to indoor residential concentrations, VOC concentrations were measured inside, outside, and, if present, in the attached garage, basement, or common hallway of an apartment of 55 residences in the Boston area, most over two seasons, as part of the Boston Exposure Assessment in Microenvironments (BEAM) Study. Of the 55 residences in the study, 11 had attached garages and basements, 24 had only basements, 10 other residences had common apartment hallways, and the remaining 10 were treated as single compartment residences. Concentrations in the garage were up to 5-10 times higher at the median than indoor concentrations for mobile source pollutants including benzene, toluene, ethylbenzene, and xylenes. Basement/indoor concentration ratios were significantly >1 for methylene chloride, ethylbenzene, m, p-xylene, and o-xylene, and summer ratios tended to be higher than winter ratios. Approximately, 20-40% of the indoor concentration for compounds associated with gasoline sources, such as methyl t-butyl ether (MTBE), benzene, toluene, ethylbenzene, and xylenes, can be attributed to an attached garage at the residence, with garages laterally attached to the first floor of the home having a larger impact. At the median, basements contributed to approximately 10-20% of the estimated indoor concentrations. For apartments, approximately 5-10% of the estimated indoor concentrations confer with air from the hallway. Contributions of these secondary zones to concentrations in the living area of a home were calculated using concentration and airflow estimates. Our findings illustrate the potential

Geophysical evaluation of groundwater potential in part of southwestern Basement Complex terrain of Nigeria

NASA Astrophysics Data System (ADS)

Bayewu, Olateju O.; Oloruntola, Moroof O.; Mosuro, Ganiyu O.; Laniyan, Temitope A.; Ariyo, Stephen O.; Fatoba, Julius O.

2017-12-01

The geophysical assessment of groundwater in Awa-Ilaporu, near Ago Iwoye southwestern Nigeria was carried out with the aim of delineating probable areas of high groundwater potential. The area falls within the Crystalline Basement Complex of southwestern Nigeria which is predominantly underlain by banded gneiss, granite gneiss and pegmatite. The geophysical investigation involves the very low frequency electromagnetic (VLF-EM) and Vertical Electrical Sounding (VES) methods. The VLF-EM survey was at 10 m interval along eight traverses ranging between 290 and 700 m in length using ABEM WADI VLF-EM unit. The VLF-EM survey was used to delineate areas with conductive/fractured zones. Twenty-three VES surveys were carried out with the use of Campus Ohmega resistivity meter at different location and at locations areas delineated as high conductive areas by VLF-EM survey. The result of VLF-EM survey along its traverse was used in delineating high conductive/fractured zones, it is, however, in agreement with the delineation of the VES survey. The VES results showed 3-4 geoelectric layers inferred as sandy topsoil, sandy clay, clayey and fractured/fresh basement. The combination of these two methods, therefore, helped in resolving the prospecting location for the groundwater yield in the study area.

Identification of 47 novel mutations in patients with Alport syndrome and thin basement membrane nephropathy.

PubMed

Weber, Stefanie; Strasser, Katja; Rath, Sabine; Kittke, Achim; Beicht, Sonja; Alberer, Martin; Lange-Sperandio, Bärbel; Hoyer, Peter F; Benz, Marcus R; Ponsel, Sabine; Weber, Lutz T; Klein, Hanns-Georg; Hoefele, Julia

2016-06-01

Alport syndrome (ATS) is a progressive hereditary nephropathy characterized by hematuria and proteinuria. It can be associated with extrarenal manifestations. In contrast, thin basement membrane nephropathy (TBMN) is characterized by microscopic hematuria, is largely asymptomatic, and is rarely associated with proteinuria and end-stage renal disease. Mutations have been identified in the COL4A5 gene in ATS and in the COL4A3 and COL4A4 genes in ATS and TBMN. To date, more than 1000 different mutations in COL4A5, COL4A3, and COL4A4 are known. In this study mutational analysis by exon sequencing and multiplex ligation-dependent probe amplification was performed in a large European cohort of families with ATS and TBMN. Molecular diagnostic testing of 216 individuals led to the detection of 47 novel mutations, thereby expanding the spectrum of known mutations causing ATS and TBMN by up to 10 and 6%, respectively, depending on the database. Remarkably, a high number of ATS patients with only single mutations in COL4A3 and COL4A4 were identified. Additionally, three ATS patients presented with synonymous sequence variants that possible affect correct mRNA splicing, as suggested by in silico analysis. The results of this study clearly broaden the genotypic spectrum of known mutations for ATS and TBMN, which will in turn now facilitate future studies into genotype-phenotype correlations. Further studies should also examine the significance of single heterozygous mutations in COL4A3 and COL4A4 and of synonymous sequence variants associated with ATS.

Uemachi flexure zone investigated by borehole database and numeical simulation

NASA Astrophysics Data System (ADS)

Inoue, N.; Kitada, N.; Takemura, K.

2014-12-01

The Uemachi fault zone extending north and south, locates in the center of the Osaka City, in Japan. The Uemachi fault is a blind reverse fault and forms the flexure zone. The effects of the Uemachi flexure zone are considered in constructing of lifelines and buildings. In this region, the geomorphological survey is difficult because of the regression of transgression. Many organizations have carried out investigations of fault structures. Various surveys have been conducted, such as seismic reflection survey in and around Osaka. Many borehole data for construction conformations have been collected and the geotechnical borehole database has been constructed. The investigation with several geological borehole data provides the subsurface geological information to the geotechnical borehole database. Various numerical simulations have been carried out to investigate the growth of a blind reverse fault in unconsolidated sediments. The displacement of the basement was given in two ways. One is based on the fault movement, such as dislocation model, the other is a movement of basement block of hanging wall. The Drucker-Prager and elastic model were used for the sediment and basement, respectively. The simulation with low and high angle fault movements, show the good agree with the actual distribution of the marine clay inferred from borehole data in the northern and southern Uemachi fault flexure zone, respectively. This research is partly funded by the Comprehensive Research on the Uemachi Fault Zone (from FY2010 to FY2012) by The Ministry of Education, Culture, Sports, Science and Technology (MEXT).

Two-dimensional basement modeling of central loop transient electromagnetic data from the central Azraq basin area, Jordan

NASA Astrophysics Data System (ADS)

Yogeshwar, P.; Tezkan, B.

2017-01-01

Thick sedimentary sequences are deposited in the central area of the Azraq basin in Jordan consisting mostly of hyper-saline clay and various evaporates. These sediment successions form the 10 km × 10 km large Azraq mudflat and are promising archives for a palaeoclimatical reconstruction. Besides palaeoclimatical research, the Azraq area is of tremendous importance to Jordan due to groundwater and mineral resources. The heavy exploitation of groundwater has lead to a drastic decline of the water table and drying out of the former Azraq Oasis. Two 7 and 5 km long transects were investigated from the periphery of the mudflat across its center using a total of 150 central loop transient electromagnetic (TEM) soundings. The scope of the survey was to detect the thickness of sedimentary deposits along both transects and to provide a basis for future drilling activities. We derive a two-dimensional model which can explain the TEM data for all soundings along each profile simultaneously. Previously uncertain depths of geological boundaries were determined along both transects. Particularly the thickness of the deposited mudflat sediments was identified and ranges from 40 m towards the periphery down to approximately 130 m at the deepest location. Besides that, the depth and lateral extent of a buried basalt layer was identified. In the basin center the groundwater is hyper-saline. The lateral extent of the saline water body was determined precisely along both transects. In order to investigate the detectability of the basement below the high conductive mudflat sediments an elaborate two-dimensional modeling study was performed. Both, the resistivity and depth of the basement were varied systematically. The basement resistivity cannot be determined precisely in most zones and may range roughly between 1 and 100 Ωm without deteriorating the misfit. In contrast to that, the depth down to the basement is detected accurately in most zones and along both transects. Varying

Subsurface Biodegradation in a Fractured Basement Reservoir, Shropshire, UK

NASA Astrophysics Data System (ADS)

Parnell, John; Baba, Mas'ud; Bowden, Stephen; Muirhead, David

2017-04-01

Subsurface Biodegradation in a Fractured Basement Reservoir, Shropshire, UK. John Parnell, Mas'ud Baba, Stephen Bowden, David Muirhead Subsurface biodegradation in current oil reservoirs is well established, but there are few examples of fossil subsurface degradation. Biomarker compositions of viscous and solid oil residues ('bitumen') in fractured Precambrian and other basement rocks below the Carboniferous cover in Shropshire, UK, show that they are variably biodegraded. High levels of 25-norhopanes imply that degradation occurred in the subsurface. Lower levels of 25-norhopanes occur in active seepages. Liquid oil trapped in fluid inclusions in mineral veins in the fractured basement confirm that the oil was emplaced fresh before subsurface degradation. A Triassic age for the veins implies a 200 million year history of hydrocarbon migration in the basement rocks. The data record microbial colonization of a fractured basement reservoir, and add to evidence in modern basement aquifers for microbial activity in deep fracture systems. Buried basement highs may be especially favourable to colonization, through channelling fluid flow to shallow depths and relatively low temperatures

Basement inheritance and salt tectonics in the SE Barents Sea: Insights from new potential field data

NASA Astrophysics Data System (ADS)

Gernigon, L.; Broenner, M.; Dumais, M. A.; Gradmann, S.; Grønlie, A.; Nasuti, A.; Roberts, D.

2017-12-01

The tectonic evolution of the former `grey zone' between Russia and Norway has so far remained poorly constrained due to a lack of geophysical data. In 2014, we carried out a new aeromagnetic survey (BASAR-14) in the southern part of the new Norwegian offshore territory. Caledonian and Timanian structures, highlighted by the new potential field data, dominate the basement patterns and have exerted a strong influence on the structure and development of the overlying basins and basement highs. Clearly associated with NW-SE-oriented Timanian trends, the Tiddlybanken Basin represents an atypical sag basin that developed at the southern edge of the Fedynsky High. Regional extension and rapid sedimentation initiated the salt tectonics in the Barents Sea in the Early Triassic. Some of the pillows became diapiric during the Early Triassic and rejuvenated during subsequent Jurassic-Tertiary episodes of regional extension and/or compression. At present, quite a few large diapiric salt domes along the Nordkapp and Tiddlybanken basins are relatively shallow, locally reaching the seabed and thus show a clear bathymetric and magnetic signature. Quantitative modelling along 2D seismic transects was also carried out to constrain the structural and basement composition of the study area. The predominant NE-SW Mesozoic trend of the Nordkapp Basin represents a major crustal hinge zone between the Finnmark Platform, poorly affected by major crustal deformation, and the Bjarmeland Platform where Late Palaeozoic rifting controlled the widespread accumulation of salt deposits in Late Carboniferous-Early Permian time. The entire structure and segmentation of the Nordkapp Basin have been influenced by the inherited basement configuration highlighted by the new aeromagnetic data. Both the Nordkapp and the Tiddlybanken basins appear to lie at the edge of a peculiar thick and rigid crustal feature that coincides with a highly magnetic region. The abrupt termination of the eastern Nordkapp

Equatorial Pacific gravity lineaments: interpretations with basement topography along seismic reflection lines

NASA Astrophysics Data System (ADS)

Mitchell, Neil C.; Davies, Huw

2018-03-01

The central equatorial Pacific is interesting for studying clues to upper mantle processes, as the region lacks complicating effects of continental remnants or major volcanic plateaus. In particular, the most recently produced maps of the free-air gravity field from satellite altimetry show in greater detail the previously reported lineaments west of the East Pacific Rise (EPR) that are aligned with plate motion over the mantle and originally suggested to have formed from mantle convection rolls. In contrast, the gravity field 600 km or farther west of the EPR reveals lineaments with varied orientations. Some are also parallel with plate motion over the mantle but others are sub-parallel with fracture zones or have other orientations. This region is covered by pelagic sediments reaching 500-600 m thickness so bathymetry is not so useful for seeking evidence for plate deformation across the lineaments. We instead use depth to basement from three seismic reflection cruises. In some segments of these seismic data crossing the lineaments, we find that the co-variation between gravity and basement depth is roughly compatible with typical densities of basement rocks (basalt, gabbro or mantle), as expected for some explanations for the lineaments (e.g., mantle convection rolls, viscous asthenospheric inter-fingering or extensional deformation). However, some other lineaments are associated with major changes in basement depth with only subtle changes in the gravity field, suggesting topography that is locally supported by varied crustal thickness. Overall, the multiple gravity lineament orientations suggest that they have multiple origins. In particular, we propose that a further asthenospheric inter-fingering instability mechanism could occur from pressure variations in the asthenosphere arising from regional topography and such a mechanism may explain some obliquely oriented gravity lineaments that have no other obvious origin.

The continuation of the Kazerun fault system across the Sanandaj-Sirjan zone (Iran)

NASA Astrophysics Data System (ADS)

Safaei, Homayon

2009-08-01

The Kazerun (or Kazerun-Qatar) fault system is a north-trending dextral strike-slip fault zone in the Zagros mountain belt of Iran. It probably originated as a structure in the Panafrican basement. This fault system played an important role in the sedimentation and deformation of the Phanerozoic cover sequence and is still seismically active. No previous studies have reported the continuation of this important and ancient fault system northward across the Sanandaj-Sirjan zone. The Isfahan fault system is a north-trending dextral strike-slip fault across the Sanandaj-Sirjan zone that passes west of Isfahan city and is here recognized for the first time. This important fault system is about 220 km long and is seismically active in the basement as well as the sedimentary cover sequence. This fault system terminates to the south near the Main Zagros Thrust and to the north at the southern boundary of the Urumieh-Dokhtar zone. The Isfahan fault system is the boundary between the northern and southern parts of Sanandaj-Sirjan zone, which have fundamentally different stratigraphy, petrology, geomorphology, and geodynamic histories. Similarities in the orientations, kinematics, and geologic histories of the Isfahan and Kazerun faults and the way they affect the magnetic basement suggest that they are related. In fact, the Isfahan fault is a continuation of the Kazerun fault across the Sanandaj-Sirjan zone that has been offset by about 50 km of dextral strike-slip displacement along the Main Zagros Thrust.

Heat flow evidence for hydrothermal circulation in the volcanic basement of subducting plates

NASA Astrophysics Data System (ADS)

Harris, R. N.; Spinelli, G. A.; Fisher, A. T.

2017-12-01

We summarize and interpret evidence for hydrothermal circulation in subducting oceanic basement from the Nankai, Costa Rica, south central Chile, Haida Gwaii, and Cascadia margins and explore the influence of hydrothermal circulation on plate boundary temperatures in these settings. Heat flow evidence for hydrothermal circulation in the volcanic basement of incoming plates includes: (a) values that are well below conductive (lithospheric) predictions due to advective heat loss, and (b) variability about conductive predictions that cannot be explained by variations in seafloor relief or thermal conductivity. We construct thermal models of these systems that include an aquifer in the upper oceanic crust that enhances heat transport via a high Nusselt number proxy for hydrothermal circulation. At the subduction zones examined, patterns of seafloor heat flow are not well fit by purely conductive simulations, and are better explained by simulations that include the influence of hydrothermal circulation. This result is consistent with the young basement ages (8-35 Ma) of the incoming igneous crust at these sites as well as results from global heat flow analyses showing a significant conductive heat flow deficit for crustal ages less than 65 Ma. Hydrothermal circulation within subducting oceanic basement can have a profound influence on temperatures close to the plate boundary and, in general, leads to plate boundary temperatures that are cooler than those where fluid flow does not occur. The magnitude of cooling depends on the permeability structure of the incoming plate and the evolution of permeability with depth and time. Resolving complex relationships between subduction processes, the permeability structure in the ocean crust, and the dynamics of hydrothermal circulation remains an interdisciplinary frontier.

Membranous nephropathy (bubbling appearance and spike formation) without immunoglobulin deposition in a patient with systemic lupus erythematosus.

PubMed

Miura, Naoto; Mori, Yuki; Yoshino, Masabumi; Suga, Norihiro; Kitagawa, Wataru; Yamada, Harutaka; Nishikawa, Kazuhiro; Imai, Hirokazu

2008-12-01

A 53-year-old Japanese man with systemic lupus erythematosus developed proteinuria and hematuria after a urinary stone episode. A light microscopic study of a kidney biopsy specimen demonstrated a bubbling appearance and spike formation of the basement membrane. Immunofluorescent studies revealed that there were no significant depositions of immunoglobulins, such as IgG (-), IgA (-), IgM (+/-), kappa light chain (+/-), lambda light chain (+/-), or C3 (-) in the glomerular capillary wall, though C1q was present as one-plus positive staining in mesangial areas. Electron microscopic studies showed that the thickness of the basement membrane varied from thin to thick without electron dense deposits, and that the cellular components of the podocyte were irregularly present in the basement membrane. Urinary protein decreased after the usage of prednisolone and mizoribine; however, proteinuria aggravated after an episode of urinary stone during the same treatment.

Diverse ages and origins of basement complexes, Luzon, Philippines

DOE Office of Scientific and Technical Information (OSTI.GOV)

Geary, E.E.; Harrison, T.M.; Heizler, M.

1988-04-01

Geological field investigations and /sup 40/Ar//sup 39/Ar ages from two basement complexes in southeast Luzon document the first known occurrences of pre-Late Cretaceous age rocks in the eastern Philippines. However, individual components within the two complexes vary in age from Late Jurassic (Caramoan basement complex) to Early Cretaceous and early Miocene (Camarines Norte-Calaguas Islands basement complex). These and other data show that southeast Luzon basement complexes are genetically diverse, and they indicate that the concept of an old, autochthonous basement in the Philippines is open to question. This supports the hypothesis that the Philippine Archipelago is an amalgamation of allochthonousmore » Mesozoic and Cenozoic island-arc, ocean-basin, and continental fragments that were assembled during the Tertiary.« less

The Campanian-Maastrichtian foraminiferal biostratigraphy of the basement sediments from the southern Pannonian Basin (Vojvodina, northern Serbia): implications for the continuation of the Eastern Vardar and Sava zones

NASA Astrophysics Data System (ADS)

Dunčić, Milena; Dulić, Ivan; Popov, Olivera; Bogićević, Goran; Vranjković, Alan

2017-04-01

Micropalaeontological and biostratigraphical studies included Campanian-Maastrichtian complexes from five oil exploration wells drilled in northern Serbia (Vojvodina): the first is a carbonate-clastic complex and second is a complex containing ophiolites intercalated with hemipelagic and pelagic sediments. Within the studied complexes, rich associations of planktonic and benthic foraminifera, calcareous nannoplankton, palynomorphs, as well as shallow and deep-water fossil detritus were determined. The presence of relatively rich associations of planktonic foraminifera allowed recognition of two biozones: the Globotruncana ventricosa Zone, observed in the sediments of the carbonate-clastic complex and the Gansserina gansseri Zone, observed in both complexes. Except biozones, based on documented index species, for some units in both complexes, larger benthic foraminifera species had special biostratigraphical value, and in some of them, the calcareous nannoplankton zones were recognized. The studied complexes represent deep-water formations, generated in oceanic island arc and trough zones. The presence of limestones, which originate from destroyed rudist reefs, is explained by transfer by means of gravitational transport mechanisms of shallow-water sediments to deep-water depositional environments. In this paper, the results of more detailed biostratigraphical and palaeo-ecological studies of foraminifera associations in Campanian-Maastrichtian complexes in Vojvodina are presented. Combined with lithological studies, seven units were determined within the complexes. The obtained results are important as a part of multidisciplinary, regional exploration of both complexes, generated in specific geological conditions, that today constitute a part of the pre-Neogene basement complex in the southeastern part of the Pannonian Basin. The Campanian- Maastrichtian carbonate-clastic complex represents sedimentary cover of the Eastern Vardar Ophiolitic Unit, while the

Basement rocks of Halmahera, eastern Indonesia: Implications for the early history of the Philippine Sea

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hall, R.G.N.; Ballantyne, P.

1990-06-01

The oldest rocks known on Halmahera, eastern Indonesia, are petrologically and chemically similar to supra-subduction ophiolites and include boninitic volcanics resembling those dredged from the Marianas forearc. The age of the ophiolitic rocks is unknown; in east Halmahera they are overlain by Late Cretaceous and Eocene volcanics and associated sediments. Similar volcanics form the basement of western Halmahera. Plutonic rocks intruding the ophiolite and associated metamorphic rocks also yield Late Cretaceous to Eocene radiometric ages. The petrology and chemistry of the igneous rocks indicate an island arc origin. These rocks are locally overlain by shallow-water Eocene limestones and all aremore » overlain unconformably by Neogene sediments. The Halmahera basement rocks have many structural, petrological, and stratigraphic similarities to submarine plateaus of the southern and northern Philippine Sea and basement terranes of the eastern Philippines. The authors suggest that these similarities indicate the existence of an extensive region of Late Cretaceous and Eocene volcanism built upon probable Mesozoic ophiolitic basement. The resultant thickened crust was later fragmented by spreading in the West Philippine Sea Central Basin and backarc spreading in the Eastern Philippine Sea. It is difficult to reconcile the present distribution of these crustal fragments with a linear arc, but equally difficult to propose a simple alternative. A proto-Philippine archipelago, with short-lived arcs separated by small oceanic basins, may be the closest modern analog. The development of younger subduction zones has been influenced by the distribution of thickened crustal fragments as they have re-amalgamated since the Miocene.« less

Structural analysis of a fractured basement reservoir, central Yemen

NASA Astrophysics Data System (ADS)

Veeningen, Resi; Rice, Hugh; Schneider, Dave; Grasemann, Bernhard; Decker, Kurt

2013-04-01

The Pan-African Arabian-Nubian Shield (ANS), within which Yemen lies, formed as a result of Neoproterozoic collisional events between c. 870-550 Ma. Several subsequent phases of extension occurred, from the Mesozoic (due to the breakup of Gondwana) to the Recent (forming the Gulf of Aden and the Red Sea). These resulted in the formation of numerous horst- and-graben structures and the development of fractured basement reservoirs in the southeast part of the ANS. Two drill cores from the Mesozoic Marib-Shabwa Basin, central Yemen, penetrated the upper part of the Pan-African basement. The cores show both a lithological and structural inhomogeneity, with variations in extension-related deformation structures such as dilatational breccias, open fractures and closed veins. At least three deformation events have been recognized: D1) Ductile to brittle NW-SE directed faulting during cooling of a granitic pluton. U-Pb zircon ages revealed an upper age limit for granite emplacement at 627±3.5 Ma. As these structures show evidence for ductile deformation, this event must have occurred during the Ediacaran, shortly after intrusion, since Rb/Sr and (U-Th)/He analyses show that subsequent re-heating of the basement did not take place. D2) The development of shallow dipping, NNE-SSW striking extensional faults that formed during the Upper Jurassic, simultaneously with the formation of the Marib-Shabwa Basin. These fractures are regularly cross-cut by D3. D3) Steeply dipping NNE-SSW to ENE-WSW veins that are consistent with the orientation of the opening of the Gulf of Aden. These faults are the youngest structures recognized. The formation of ductile to brittle faults in the granite (D1) resulted in a hydrothermally altered zone ca. 30 cm wide replacing (mainly) plagioclase with predominantly chlorite, as well as kaolinite and heavy element minerals such as pyrite. The alteration- induced porosity has an average value of 20%, indicating that the altered zone is potentially a

Staged membrane oxidation reactor system

DOEpatents

Repasky, John Michael; Carolan, Michael Francis; Stein, VanEric Edward; Chen, Christopher Ming-Poh

2014-05-20

Ion transport membrane oxidation system comprising (a) two or more membrane oxidation stages, each stage comprising a reactant zone, an oxidant zone, one or more ion transport membranes separating the reactant zone from the oxidant zone, a reactant gas inlet region, a reactant gas outlet region, an oxidant gas inlet region, and an oxidant gas outlet region; (b) an interstage reactant gas flow path disposed between each pair of membrane oxidation stages and adapted to place the reactant gas outlet region of a first stage of the pair in flow communication with the reactant gas inlet region of a second stage of the pair; and (c) one or more reactant interstage feed gas lines, each line being in flow communication with any interstage reactant gas flow path or with the reactant zone of any membrane oxidation stage receiving interstage reactant gas.

Staged membrane oxidation reactor system

DOEpatents

Repasky, John Michael; Carolan, Michael Francis; Stein, VanEric Edward; Chen, Christopher Ming-Poh

2013-04-16

Ion transport membrane oxidation system comprising (a) two or more membrane oxidation stages, each stage comprising a reactant zone, an oxidant zone, one or more ion transport membranes separating the reactant zone from the oxidant zone, a reactant gas inlet region, a reactant gas outlet region, an oxidant gas inlet region, and an oxidant gas outlet region; (b) an interstage reactant gas flow path disposed between each pair of membrane oxidation stages and adapted to place the reactant gas outlet region of a first stage of the pair in flow communication with the reactant gas inlet region of a second stage of the pair; and (c) one or more reactant interstage feed gas lines, each line being in flow communication with any interstage reactant gas flow path or with the reactant zone of any membrane oxidation stage receiving interstage reactant gas.

Staged membrane oxidation reactor system

DOEpatents

Repasky, John Michael; Carolan, Michael Francis; Stein, VanEric Edward; Chen, Christopher Ming-Poh

2012-09-11

Ion transport membrane oxidation system comprising (a) two or more membrane oxidation stages, each stage comprising a reactant zone, an oxidant zone, one or more ion transport membranes separating the reactant zone from the oxidant zone, a reactant gas inlet region, a reactant gas outlet region, an oxidant gas inlet region, and an oxidant gas outlet region; (b) an interstage reactant gas flow path disposed between each pair of membrane oxidation stages and adapted to place the reactant gas outlet region of a first stage of the pair in flow communication with the reactant gas inlet region of a second stage of the pair; and (c) one or more reactant interstage feed gas lines, each line being in flow communication with any interstage reactant gas flow path or with the reactant zone of any membrane oxidation stage receiving interstage reactant gas.

Fuzzy logic modeling of the resistivity parameter and topography features for aquifer assessment in hydrogeological investigation of a crystalline basement complex

NASA Astrophysics Data System (ADS)

Adabanija, M. A.; Omidiora, E. O.; Olayinka, A. I.

2008-05-01

A linguistic fuzzy logic system (LFLS)-based expert system model has been developed for the assessment of aquifers for the location of productive water boreholes in a crystalline basement complex. The model design employed a multiple input/single output (MISO) approach with geoelectrical parameters and topographic features as input variables and control crisp value as the output. The application of the method to the data acquired in Khondalitic terrain, a basement complex in Vizianagaram District, south India, shows that potential groundwater resource zones that have control output values in the range 0.3295-0.3484 have a yield greater than 6,000 liters per hour (LPH). The range 0.3174-0.3226 gives a yield less than 4,000 LPH. The validation of the control crisp value using data acquired from Oban Massif, a basement complex in southeastern Nigeria, indicates a yield less than 3,000 LPH for control output values in the range 0.2938-0.3065. This validation corroborates the ability of control output values to predict a yield, thereby vindicating the applicability of linguistic fuzzy logic system in siting productive water boreholes in a basement complex.

Localization of type IV collagen a 1 to a 6 chains in basement membrane during mouse molar germ development.

PubMed

Nagai, N; Nakano, K; Sado, Y; Naito, I; Gunduz, M; Tsujigiwa, H; Nagatsuka, H; Ninomiya, Y; Siar, C H

2001-10-01

The dental basement membrane (BM) putatively mediates epithelial-mesenchymal interactions during tooth morphogenesis and cytodifferentiation. Type IV collagen alpha chains, a major network-forming protein of the dental BM, was studied and results disclosed distinct expression patterns at different stages of mouse molar germ development. At the dental placode and bud stage, the BM of the oral epithelium expressed alpha 1, alpha 2, alpha 5 and alpha 6 chains while the gubernaculum dentis, in addition to the above four chains, also expressed a 4 chain. An asymmetrical expression for alpha 4, alpha 5 and alpha 6 chains was observed at the bud stage. At the early bell stage, the BM associated with the inner enamel epithelium (IEE) of molar germ expressed alpha 1, alpha 2 and alpha 4 chains while the BM of the outer enamel epithelium (OEE) expressed only alpha 1 and a 2 chains. With the onset of dentinogenesis, the collagen a chain profile of the IEE BM gradually disappeared. Howeverfrom the early to late bell stage, the gubernaculum dentis consistently expressed alpha 1, alpha 2, alpha 5 and a 6 chains resembling fetal oral mucosa. These findings suggest that stage- and position-specific distribution of type IV collagen alpha subunits occur during molar germ development and that these changes are essential for molar morphogenesis and cytodifferentiation.

The pectinate zone is stiff and the arcuate zone determines passive basilar membrane mechanics in the gerbil

NASA Astrophysics Data System (ADS)

Xia, Hongyi; Steele, Charles R.; Puria, Sunil

2018-05-01

The gerbil basilar membrane (BM) differs from other mammalian BMs in that the lower collagen-fiber layer of the pectinate zone (PZ) forms an arch, the upper fiber layer is flat, and ground substance separates the two layers. The role of this arch has been unknown, but can be elucidated by models. In the standard simple beam model (SBM), the upper and lower collagen-fiber layers of the BM are represented as a single layer in both the PZ and the arcuate zone (AZ). In our new arch-beam model (ABM), the upper fiber layer is flat, the lower layer forms an arch in the PZ, and the two layers combine to form the flat portion of the BM in the AZ. This design is incorporated into a 3D finite-element tapered-box model of the cochlea with viscous fluid. We find in the model that the PZ rotates as a rigid body, so its specific properties have little influence, while the AZ thickness and collagen volume fraction primarily determine passive BM mechanics.

Magnetic Basement Depth Inversion in the Space Domain

NASA Astrophysics Data System (ADS)

Nunes, Tiago Mane; Barbosa, Valéria Cristina F.; Silva, João Batista C.

2008-10-01

We present a total-field anomaly inversion method to determine both the basement relief and the magnetization direction (inclination and declination) of a 2D sedimentary basin presuming negligible sediment magnetization. Our method assumes that the magnetic intensity contrast is constant and known. We use a nonspectral approach based on approximating the vertical cross section of the sedimentary basin by a polygon, whose uppermost vertices are forced to coincide with the basin outcrop, which are presumably known. For fixed values of the x coordinates our method estimates the z coordinates of the unknown polygon vertices. To obtain the magnetization direction we assume that besides the total-field anomaly, information about the basement’s outcrops at the basin borders and the basement depths at a few points is available. To obtain stable depth-to-basement estimates we impose overall smoothness and positivity constraints on the parameter estimates. Tests on synthetic data showed that the simultaneous estimation of the irregular basement relief and the magnetization direction yields good estimates for the relief despite the mild instability in the magnetization direction. The inversion of aeromagnetic data from the onshore Almada Basin, Brazil, revealed a shallow, eastward-dipping basement basin.

Glomerular basement membrane injuries in IgA nephropathy evaluated by double immunostaining for α5(IV) and α2(IV) chains of type IV collagen and low-vacuum scanning electron microscopy.

PubMed

Masuda, Yukinari; Yamanaka, Nobuaki; Ishikawa, Arimi; Kataoka, Mitue; Arai, Takashi; Wakamatsu, Kyoko; Kuwahara, Naomi; Nagahama, Kiyotaka; Ichikawa, Kaori; Shimizu, Akira

2015-06-01

The glomerulus contains well-developed capillaries, which are at risk of injury due to high hydrostatic pressure, hyperfiltration, hypertension and inflammation. However, the pathological alterations of the injured glomerular basement membrane (GBM), the main component of the glomerular filtration barrier, are still uncertain in cases of glomerulonephritis. We examined the alterations of the GBM in 50 renal biopsy cases with IgA nephropathy (31.8 ± 17.6 years old) using double immunostaining for the α2(IV) and α5(IV) chains of type IV collagen, and examining the ultrastructural alterations by transmission electron microscopy (TEM) and low-vacuum scanning electron microscopy (LV-SEM). The GBM of IgA nephropathy cases showed various morphological and qualitative alterations. In the TEM findings, thinning, gaps, rupture, thickening with a lamellar and reticular structure and double contours were detected in the GBM. Double immunostaining for α5(IV) and α2(IV) showed thickening of the GBM with reduced α5(IV) and increased α2(IV), or mosaic images of α5(IV) and α2(IV), and holes, fractures, spiny projections and rupture of α5(IV) in the GBM. In addition, LV-SEM showed an etched image and multiple holes in a widening and wavy GBM. These findings might be associated with the development of a brittle GBM in IgA nephropathy. Glomerular basement membrane alterations were frequently noted in IgA nephropathy, and were easily evaluated by double immunostaining for α2(IV) and α5(IV) of type IV collagen and LV-SEM. The application of these analyses to human renal biopsy specimens may enhance our understanding of the alterations of the GBM that occur in human glomerular diseases.

Contour mapping of relic structures in the Precambrian basement of the Reelfoot rift, North American midcontinent

USGS Publications Warehouse

Dart, R.L.; Swolfs, H.S.

1998-01-01

A new contour map of the basement of the Reelfoot rift constructed from drill hole and seismic reflection data shows the general surface configuration as well as several major and minor structural features. The major features are two asymmetric intrarift basins, bounded by three structural highs, and the rift margins. The basins are oriented normal to the northeast trend of the rift. Two of the highs appear to be ridges of undetermined width that extend across the rift. The third high is an isolated dome or platform located between the basins. The minor features are three linear structures of low relief oriented subparallel to the trend of the rift. Two of these, located within the rift basins, may divide the rift basins into paired subbasins. These mapped features may be the remnants of initial extensional rifting, half graben faulting, and basement subsidence. The rift basins are interpreted as having formed as opposing half graben, and the structural highs are interpreted as having formed as associated accommodation zones. Some of these features appear to be reactivated seismogenic structures within the modem midcontinent compressional stress regime. A detailed knowledge of the geometries of the Reelfoot rift's basement features, therefore, is essential when evaluating their seismic risk potential.

Geo-electrical investigation of near surface conductive structures suitable for groundwater accumulation in a resistive crystalline basement environment: A case study of Isuada, southwestern Nigeria

NASA Astrophysics Data System (ADS)

Kayode, J. S.; Adelusi, A. O.; Nawawi, M. N. M.; Bawallah, M.; Olowolafe, T. S.

2016-07-01

This paper presents a geophysical surveying for groundwater identification in a resistive crystalline basement hard rock in Isuada area, Southwestern Nigeria. Very low frequency (VLF) electromagnetic and electrical resistivity geophysical techniques combined with well log were used to characterize the concealed near surface conductive structures suitable for groundwater accumulation. Prior to this work; little was known about the groundwater potential of this area. Qualitative and semi-quantitative interpretations of the data collected along eight traverses at 20 m spacing discovered conductive zones suspected to be fractures, faults, and cracks which were further mapped using Vertical Electrical Sounding (VES) technique. Forty VES stations were utilized using Schlumberger configurations with AB/2 varying from 1 to 100 m. Four layers i.e. the top soil, the weathered layer, the partially weathered/fractured basement and the fresh basement were delineated from the interpreted resistivity curves. The weathered layers constitute the major aquifer unit in the area and are characterized by moderately low resistivity values which ranged between about 52 Ωm and 270 Ωm while the thickness varied from 1 to 35 m. The depth to the basement and the permeable nature of the weathered layer obtained from both the borehole and the hand-dug wells was used to categorize the groundwater potential of the study area into high, medium and low ratings. The groundwater potential map revealed that about 45% of the study area falls within the low groundwater potential rating while about 10% constitutes the medium groundwater potential and the remaining 45% constitutes high groundwater potential. The low resistivity, thick overburden, and fractured bedrock constitute the aquifer units and the series of basement depressions identified from the geoelectric sections as potential conductive zones appropriate for groundwater development.

Distribution of Basement Membrane Molecules, Laminin and Collagen Type IV, in Normal and Degenerated Cartilage Tissues

PubMed Central

Toh, Wei Seong; Gomoll, Andreas H.; Olsen, Bjørn Reino; Spector, Myron

2014-01-01

Objective: The objective of the present study was to investigate the presence and distribution of 2 basement membrane (BM) molecules, laminin and collagen type IV, in healthy and degenerative cartilage tissues. Design: Normal and degenerated tissues were obtained from goats and humans, including articular knee cartilage, the intervertebral disc, and meniscus. Normal tissue was also obtained from patella-tibial enthesis in goats. Immunohistochemical analysis was performed using anti-laminin and anti–collagen type IV antibodies. Human and goat skin were used as positive controls. The percentage of cells displaying the pericellular presence of the protein was graded semiquantitatively. Results: When present, laminin and collagen type IV were exclusively found in the pericellular matrix, and in a discrete layer on the articulating surface of normal articular cartilage. In normal articular (hyaline) cartilage in the human and goat, the proteins were found co-localized pericellularly. In contrast, in human osteoarthritic articular cartilage, collagen type IV but not laminin was found in the pericellular region. Nonpathological fibrocartilaginous tissues from the goat, including the menisci and the enthesis, were also positive for both laminin and collagen type IV pericellularly. In degenerated fibrocartilage, including intervertebral disc, as in degenerated hyaline cartilage only collagen type IV was found pericellularly around chondrocytes but with less intense staining than in non-degenerated tissue. In calcified cartilage, some cells were positive for laminin but not type IV collagen. Conclusions: We report differences in expression of the BM molecules, laminin and collagen type IV, in normal and degenerative cartilaginous tissues from adult humans and goats. In degenerative tissues laminin is depleted from the pericellular matrix before collagen type IV. The findings may inform future studies of the processes underlying cartilage degeneration and the functional

Distribution of Basement Membrane Molecules, Laminin and Collagen Type IV, in Normal and Degenerated Cartilage Tissues.

PubMed

Foldager, Casper Bindzus; Toh, Wei Seong; Gomoll, Andreas H; Olsen, Bjørn Reino; Spector, Myron

2014-04-01

The objective of the present study was to investigate the presence and distribution of 2 basement membrane (BM) molecules, laminin and collagen type IV, in healthy and degenerative cartilage tissues. Normal and degenerated tissues were obtained from goats and humans, including articular knee cartilage, the intervertebral disc, and meniscus. Normal tissue was also obtained from patella-tibial enthesis in goats. Immunohistochemical analysis was performed using anti-laminin and anti-collagen type IV antibodies. Human and goat skin were used as positive controls. The percentage of cells displaying the pericellular presence of the protein was graded semiquantitatively. When present, laminin and collagen type IV were exclusively found in the pericellular matrix, and in a discrete layer on the articulating surface of normal articular cartilage. In normal articular (hyaline) cartilage in the human and goat, the proteins were found co-localized pericellularly. In contrast, in human osteoarthritic articular cartilage, collagen type IV but not laminin was found in the pericellular region. Nonpathological fibrocartilaginous tissues from the goat, including the menisci and the enthesis, were also positive for both laminin and collagen type IV pericellularly. In degenerated fibrocartilage, including intervertebral disc, as in degenerated hyaline cartilage only collagen type IV was found pericellularly around chondrocytes but with less intense staining than in non-degenerated tissue. In calcified cartilage, some cells were positive for laminin but not type IV collagen. We report differences in expression of the BM molecules, laminin and collagen type IV, in normal and degenerative cartilaginous tissues from adult humans and goats. In degenerative tissues laminin is depleted from the pericellular matrix before collagen type IV. The findings may inform future studies of the processes underlying cartilage degeneration and the functional roles of these 2 extracellular matrix proteins

Air exchange rates and migration of VOCs in basements and residences

PubMed Central

Du, Liuliu; Batterman, Stuart; Godwin, Christopher; Rowe, Zachary; Chin, Jo-Yu

2015-01-01

Basements can influence indoor air quality by affecting air exchange rates (AERs) and by the presence of emission sources of volatile organic compounds (VOCs) and other pollutants. We characterized VOC levels, AERs and interzonal flows between basements and occupied spaces in 74 residences in Detroit, Michigan. Flows were measured using a steady-state multi-tracer system, and 7-day VOC measurements were collected using passive samplers in both living areas and basements. A walkthrough survey/inspection was conducted in each residence. AERs in residences and basements averaged 0.51 and 1.52 h−1, respectively, and had strong and opposite seasonal trends, e.g., AERs were highest in residences during the summer, and highest in basements during the winter. Air flows from basements to occupied spaces also varied seasonally. VOC concentration distributions were right-skewed, e.g., 90th percentile benzene, toluene, naphthalene and limonene concentrations were 4.0, 19.1, 20.3 and 51.0 μg m−3, respectively; maximum concentrations were 54, 888, 1117 and 134 μg m−3. Identified VOC sources in basements included solvents, household cleaners, air fresheners, smoking, and gasoline-powered equipment. The number and type of potential VOC sources found in basements are significant and problematic, and may warrant advisories regarding the storage and use of potentially strong VOCs sources in basements. PMID:25601281

NATURAL BASEMENT VENTILATION AS A RADON MITIGATION TECHNIQUE

EPA Science Inventory

The report documents a study of natural basement ventilation in two research houses during both the summer cooling season and the winter heating season. NOTE: Natural basement ventilation has always been recommended as a way to reduce radon levels in houses. However, its efficacy...

Histological analysis of retraction pocket pars tensa of tympanic membrane in children.

PubMed

Urík, M; Hurník, P; Žiak, D; Machač, J; Šlapák, I; Motyka, O; Vaculová, J; Dvořáčková, J

2016-07-01

Histological and histochemical analysis of retraction pocket of pars tensa of tympanic membrane in children. Identification of morphological abnormalities in comparison with a healthy tympanic membrane as it is described in standard textbook. Identification of signs typical for cholesteatoma and support for a retraction theory of cholesteatoma formation. A prospective study analysing 31 samples of retraction pockets taken during surgery. University Hospital, Children's Medical Centre Samples of retraction pockets were processed by a standard process for light microscopy, stained by haematoxylin-eosin. Van Gieson's stain was used for differential staining of collagen, Verhoeff's stain for elastic fibre tissues, Alcian blue for acidic polysaccharides and PAS (Periodic Acid Schiff) method for basement membrane polysaccharides. The following findings were observed in the samples of retraction pockets: hyperkeratosis (100%), hypervascularisations (100%), subepithelial fragmented elastic fibres (96%), myxoid changes (87%), subepithelial inflammatory infiltration (84%), rete pegs (71%), papilomatosis (71%), intraepithelial inflammatory cellularizations, (48%), intraepithelial spongiosis (16%) and parakeratosis (3%). No basement membrane continuity interruptions were observed. Thickness of retraction pocket, thickness of epidermis, occurrence of rete pegs and frequency of fragmented elastic fibres was higher in a Grade III stage RP than Grade II stage RP (according to Charachon). Morphological abnormalities in the structure of retraction pockets in comparison with a healthy tympanic membrane were described. The changes are typical for a structure of cholesteatoma (these changes are common in matrix and perimatrix), supporting retraction theory of its origin. Our observations show that it is inflammation that probably plays a key role in the pathogenesis of retraction pocket. The frequency of some of the changes increases with the stage of retraction pocket (II

Prevention of retinal capillary basement membrane thickening in diabetic dogs by a non-steroidal anti-inflammatory drug.

PubMed

Gardiner, T A; Anderson, H R; Degenhardt, T; Thorpe, S R; Baynes, J W; Archer, D B; Stitt, A W

2003-09-01

To investigate the effect of treatment with the non-steroidal anti-inflammatory drug Sulindac on the early vascular pathology of diabetic retinopathy in the dog, and it's effect on recognised biochemical indices of hyperglycaemia-related pathophysiology. Experimental diabetes (streptozotocin/alloxan) was induced in 22 male beagle dogs and 12 of the animals were assigned at random to receive oral Sulindac (10 mg/kg daily). Age- and sex-matched control animals were maintained as non-diabetic controls. After 4 years, several morphological parameters were quantified in the retinal microvasculature of each animal group using an established stereological method. Also, the following diabetes-associated biochemical parameters were analysed: accumulation of advanced glycation end products (AGEs), red blood cell polyol levels and antioxidant status. Diabetes increased red blood cell sorbitol levels when compared to non-diabetic controls (p< or =0.05), however, there was no difference in sorbitol levels between the untreated and the treated diabetic animals. No significant differences were found in red blood cell myoinositol levels between the three groups of animals. Pentosidine and other AGEs were increased two- to three-fold in the diabetic animals (p< or =0.001) although treatment with Sulindac did not affect their accumulation in diabetic skin collagen or alter diabetes-induced rises in plasma malondialdehyde. Retinal capillary basement membrane volume was significantly increased in the untreated diabetic dogs compared to non-diabetic controls or Sulindac-treated diabetic animals (p< or =0.0001). This study has confirmed the beneficial effect of a non-steroidal anti-inflammatory drug on the early vascular pathology of diabetic retinopathy. However the treatment benefit was not dependent on inhibition of polyol pathway activity, advanced glycation, or oxidative stress.

Hindered transport of macromolecules in isolated glomeruli. II. Convection and pressure effects in basement membrane.

PubMed

Edwards, A; Daniels, B S; Deen, W M

1997-01-01

The filtration rates for water and a polydisperse mixture of Ficoll across films of isolated glomerular basement membrane (GBM) were measured to characterize convective transport across this part of the glomerular capillary wall. Glomeruli were isolated from rat kidneys and the cells were removed by detergent lysis, leaving a preparation containing almost pure GBM that could be consolidated into a layer at the base of a small ultrafiltration cell. A Ficoll mixture with Stokes-Einstein radii ranging from about 2.0 to 7.0 nm was labeled with fluorescein, providing a set of rigid, spherical test macromolecules with little molecular charge. Filtration experiments were performed at two physiologically relevant hydraulic pressure differences (delta P), 35 and 60 mmHg. The sieving coefficient (filtrate-to-retentate concentration ratio) for a given size of Ficoll tended to be larger at 35 than at 60 mmHg, the changes being greater for the smaller molecules. The Darcy permeability also varied inversely with pressure, averaging 1.48 +/- 0.10 nm2 at 35 mmHg and 0.82 +/- 0.07 nm2 at 60 mmHg. Both effects could be explained most simply by postulating that the intrinsic permeability properties of the GBM change in response to compression. The sieving data were consistent with linear declines in the hindrance factors for convection and diffusion with increasing pressure, and correlations were derived to relate those hindrance factors to molecular size and delta P. Comparisons with previous Ficoll sieving data for rats in vivo suggest that the GBM is less size-restrictive than the cell layers, but that its contribution to the overall size selectivity of the barrier is not negligible. Theoretical predictions of the Darcy permeability based on a model in which the GBM is a random fibrous network consisting of two populations of fibers were in excellent agreement with the present data and with ultrastructural observations in the literature.

Polymetamorphic evolution of the granulite-facies Paleoproterozoic basement of the Kabul Block, Afghanistan

NASA Astrophysics Data System (ADS)

Collett, Stephen; Faryad, Shah Wali; Mosazai, Amir Mohammad

2015-08-01

The Kabul Block is an elongate crustal fragment which cuts across the Afghan Central Blocks, adjoining the Indian and Eurasian continents. Bounded by major strike slip faults and ophiolitic material thrust onto either side, the block contains a strongly metamorphosed basement consisting of some of the only quantifiably Proterozoic rocks south of the Herat-Panjshir Suture Zone. The basement rocks crop-out extensively in the vicinity of Kabul City and consist predominantly of migmatites, gneisses, schists and small amounts of higher-grade granulite-facies rocks. Granulite-facies assemblages were identified in felsic and mafic siliceous rocks as well as impure carbonates. Granulite-facies conditions are recorded by the presence of orthopyroxene overgrowing biotite in felsic rocks; by orthopyroxene overgrowing amphibole in mafic rocks and by the presence of olivine and clinohumite in the marbles. The granulite-facies assemblages are overprinted by a younger amphibolite-facies event that is characterized by the growth of garnet at the expense of the granulite-facies phases. Pressure-temperature (P-T) conditions for the granulite-facies event of around 850 °C and up to 7 kbar were calculated through conventional thermobarometry and phase equilibria modeling. The younger, amphibolite-facies event shows moderately higher pressures of up to 8.5 kbar at around 600 °C. This metamorphism likely corresponds to the dominant metamorphic event within the basement of the Kabul Block. The results of this work are combined with the litho-stratigraphic relations and recent geochronological dating to analyze envisaged Paleoproterozoic and Neoproterozoic metamorphic events in the Kabul Block.

A skin substitute based on human amniotic membrane.

PubMed

Tauzin, Hélène; Rolin, Gwenaël; Viennet, Céline; Saas, Philippe; Humbert, Philippe; Muret, Patrice

2014-06-01

Human amniotic membrane (HAM) has biological properties which are useful for wound healing. HAM is notably one of the therapeutic alternatives for venous leg ulcer care. Indeed, a prospective clinical study has demonstrated that cryopreserved HAM transplantation for leg ulcer is feasible, safe and has beneficial effects: 80 % of the patients had a significant clinical response. Nevertheless, at the end of the 3-month follow-up period, only 20 % of the ulcers were totally closed. The aim of this work was to create and characterize a model of epidermized HAM. The method of HAM desepithelialization was validated by histology, immunohistochemistry and scanning electron microscopy. Then, de-epithelialized HAM was seeded with primary keratinocytes. After 21 days of culture, 15 at the air-liquid interface, the model obtained was analyzed histologically and by immunohistochemistry. The amniotic basement membrane was preserved during enzymatic desepithelialization of HAM. Primary keratinocytes proliferated on HAM: the model obtained showed involucrin expression and had a good basement membrane. As re-epithelialization is an important step for ulcer closure, a model of epidermized HAM could be used to speed up the healing of such wounds.

Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype.

PubMed

Yang, Shaowei; Sun, Yexiao; Geng, Zhijun; Ma, Kui; Sun, Xiaoyan; Fu, Xiaobing

2016-05-01

The majority of studies on scar formation have mainly focused on the dermis and little is known of the involvement of the epidermis. Previous research has demonstrated that the scar tissue-derived keratinocytes are different from normal cells at both the genetic and cell biological levels; however, the mechanisms responsible for the fundamental abnormalities in keratinocytes during scar development remain elusive. For this purpose, in this study, we used normal, wound edge and hypertrophic scar tissue to examine the morphological changes which occur during epidermal regeneration as part of the wound healing process and found that the histological structure of hypertrophic scar tissues differed from that of normal skin, with a significant increase in epidermal thickness. Notably, staining of the basement membrane (BM) appeared to be absent in the scar tissues. Moreover, immunofluorescence staining for cytokeratin (CK)10, CK14, CK5, CK19 and integrin-β1 indicated the differential expression of cell markers in the epidermal keratinocytes among the normal, wound edge and hypertrophic scar tissues, which corresponded with the altered BM structures. By using a panel of proteins associated with BM components, we validated our hypothesis that the BM plays a significant role in regulating the cell fate decision of epidermal keratinocytes during skin wound healing. Alterations in the structure of the BM promote basal keratinocytes to adopt a proliferative phenotype both in vivo and in vitro.

Crucial Role of Mesangial Cell-derived Connective Tissue Growth Factor in a Mouse Model of Anti-Glomerular Basement Membrane Glomerulonephritis

PubMed Central

Toda, Naohiro; Mori, Kiyoshi; Kasahara, Masato; Ishii, Akira; Koga, Kenichi; Ohno, Shoko; Mori, Keita P.; Kato, Yukiko; Osaki, Keisuke; Kuwabara, Takashige; Kojima, Katsutoshi; Taura, Daisuke; Sone, Masakatsu; Matsusaka, Taiji; Nakao, Kazuwa; Mukoyama, Masashi; Yanagita, Motoko; Yokoi, Hideki

2017-01-01

Connective tissue growth factor (CTGF) coordinates the signaling of growth factors and promotes fibrosis. Neonatal death of systemic CTGF knockout (KO) mice has hampered analysis of CTGF in adult renal diseases. We established 3 types of CTGF conditional KO (cKO) mice to investigate a role and source of CTGF in anti-glomerular basement membrane (GBM) glomerulonephritis. Tamoxifen-inducible systemic CTGF (Rosa-CTGF) cKO mice exhibited reduced proteinuria with ameliorated crescent formation and mesangial expansion in anti-GBM nephritis after induction. Although CTGF is expressed by podocytes at basal levels, podocyte-specific CTGF (pod-CTGF) cKO mice showed no improvement in renal injury. In contrast, PDGFRα promoter-driven CTGF (Pdgfra-CTGF) cKO mice, which predominantly lack CTGF expression by mesangial cells, exhibited reduced proteinuria with ameliorated histological changes. Glomerular macrophage accumulation, expression of Adgre1 and Ccl2, and ratio of M1/M2 macrophages were all reduced both in Rosa-CTGF cKO and Pdgfra-CTGF cKO mice, but not in pod-CTGF cKO mice. TGF-β1-stimulated Ccl2 upregulation in mesangial cells and macrophage adhesion to activated mesangial cells were decreased by reduction of CTGF. These results reveal a novel mechanism of macrophage migration into glomeruli with nephritis mediated by CTGF derived from mesangial cells, implicating the therapeutic potential of CTGF inhibition in glomerulonephritis. PMID:28191821

Crucial Role of Mesangial Cell-derived Connective Tissue Growth Factor in a Mouse Model of Anti-Glomerular Basement Membrane Glomerulonephritis.

PubMed

Toda, Naohiro; Mori, Kiyoshi; Kasahara, Masato; Ishii, Akira; Koga, Kenichi; Ohno, Shoko; Mori, Keita P; Kato, Yukiko; Osaki, Keisuke; Kuwabara, Takashige; Kojima, Katsutoshi; Taura, Daisuke; Sone, Masakatsu; Matsusaka, Taiji; Nakao, Kazuwa; Mukoyama, Masashi; Yanagita, Motoko; Yokoi, Hideki

2017-02-13

Connective tissue growth factor (CTGF) coordinates the signaling of growth factors and promotes fibrosis. Neonatal death of systemic CTGF knockout (KO) mice has hampered analysis of CTGF in adult renal diseases. We established 3 types of CTGF conditional KO (cKO) mice to investigate a role and source of CTGF in anti-glomerular basement membrane (GBM) glomerulonephritis. Tamoxifen-inducible systemic CTGF (Rosa-CTGF) cKO mice exhibited reduced proteinuria with ameliorated crescent formation and mesangial expansion in anti-GBM nephritis after induction. Although CTGF is expressed by podocytes at basal levels, podocyte-specific CTGF (pod-CTGF) cKO mice showed no improvement in renal injury. In contrast, PDGFRα promoter-driven CTGF (Pdgfra-CTGF) cKO mice, which predominantly lack CTGF expression by mesangial cells, exhibited reduced proteinuria with ameliorated histological changes. Glomerular macrophage accumulation, expression of Adgre1 and Ccl2, and ratio of M1/M2 macrophages were all reduced both in Rosa-CTGF cKO and Pdgfra-CTGF cKO mice, but not in pod-CTGF cKO mice. TGF-β1-stimulated Ccl2 upregulation in mesangial cells and macrophage adhesion to activated mesangial cells were decreased by reduction of CTGF. These results reveal a novel mechanism of macrophage migration into glomeruli with nephritis mediated by CTGF derived from mesangial cells, implicating the therapeutic potential of CTGF inhibition in glomerulonephritis.

Grenville age of basement rocks in Cape May NJ well: New evidence for Laurentian crust in U.S. Atlantic Coastal Plain basement Chesapeake terrane

USGS Publications Warehouse

Sheridan, R.E.; Maguire, T.J.; Feigenson, M.D.; Patino, L.C.; Volkert, R.A.

1999-01-01

The Chesapeake terrane of the U.S. mid-Atlantic Coastal Plain basement is bounded on the northwest by the Salisbury positive gravity and magnetic anomaly and extends to the southeast as far as the Atlantic coast. It underlies the Coastal Plain of Virginia, Maryland, Delaware and southern New Jersey. Rubidium/Strontium dating of the Chesapeake terrane basement yields an age of 1.025 ?? 0.036 Ga. This age is typical of Grenville province rocks of the Middle to Late Proterozoic Laurentian continent. The basement lithologies are similar to some exposed Grenville-age rocks of the Appalachians. The TiO2 and Zr/P2O5 composition of the metagabbro from the Chesapeake terrane basement is overlapped by those of the Proterozoic mafic dikes in the New Jersey Highlands. These new findings support the interpretation that Laurentian basement extends southeast as far as the continental shelf in the U.S. mid-Atlantic region. The subcrop of Laurentian crust under the mid-Atlantic Coastal Plain implies unroofing by erosion of the younger Carolina (Avalon) supracrustal terrane. Dextral-transpression fault duplexes may have caused excessive uplift in the Salisbury Embayment area during the Alleghanian orogeny. This extra uplift in the Salisbury area may have caused the subsequent greater subsidence of the Coastal Plain basement in the embayment.

Structure of the active rift zone and margins of the northern Imperial Valley from Salton Seismic Imaging Project (SSIP) data

NASA Astrophysics Data System (ADS)

Livers, A.; Han, L.; Delph, J. R.; White-Gaynor, A. L.; Petit, R.; Hole, J. A.; Stock, J. M.; Fuis, G. S.

2012-12-01

First-arrival refraction data were used to create a seismic velocity model of the upper crust across the actively rifting northern Imperial Valley and its margins. The densely sampled seismic refraction data were acquired by the Salton Seismic Imaging Project (SSIP) , which is investigating rift processes in the northern-most rift segment of the Gulf of California extensional province and earthquake hazards at the southern end of the San Andreas Fault system. A 95-km long seismic line was acquired across the northern Imperial Valley, through the Salton Sea geothermal field, parallel to the five Salton Butte volcanoes and perpendicular to the Brawley Seismic Zone and major strike-slip faults. Nineteen explosive shots were recorded with 100 m seismometer spacing across the valley and with 300-500 m spacing into the adjacent ranges. First-arrival travel times were picked from shot gathers along this line and a seismic velocity model was produced using tomographic inversion. Sedimentary basement and seismic basement in the valley are interpreted to be sediment metamorphosed by the very high heat flow. The velocity model shows that this basement to the west of the Brawley Seismic Zone is at ~4-km depth. The basement shallows to ~2-km depth in the active geothermal field and Salton Buttes volcanic field which locally coincide with the Brawley Seismic Zone. At the eastern edge of the geothermal field, the basement drops off again to ~3.5-km depth. The eastern edge of the valley appears to be fault bounded by the along-strike extension of the Sand Hills Fault, an inactive strike-slip fault. The seismic velocities to the east of the fault correspond to metamorphic rock of the Chocolate Mountains, different from the metamorphosed basement in the valley. The western edge of the valley appears to be fault bounded by the active Superstition Hills Fault. To the west of the valley, >4-km deep valley basement extends to the active Superstition Hills Fault. Basement then shallows

FOVEAL AVASCULAR ZONE AREA AFTER INTERNAL LIMITING MEMBRANE PEELING FOR EPIRETINAL MEMBRANE AND MACULAR HOLE COMPARED WITH THAT OF FELLOW EYES AND HEALTHY CONTROLS.

PubMed

Kumagai, Kazuyuki; Furukawa, Mariko; Suetsugu, Tetsuyuki; Ogino, Nobuchika

2017-08-01

To measure the foveal avascular zone (FAZ) area after internal limiting membrane (ILM) peeling and to determine the factors significantly correlated with the FAZ area. This was a retrospective, observational, and cross-sectional study. The affected and normal fellow eyes of 102 patients with unilateral macular diseases and 169 healthy subjects were studied. The patients underwent successful vitrectomy with internal limiting membrane peeling for an epiretinal membrane (n = 56) or a macular hole (n = 46). The superficial FAZ area and average foveal (within 1 mm) thickness were measured. The main outcome measures were the en face FAZ area measured in the optical coherence tomography angiographic images. The FAZ area in the epiretinal membrane group (0.148 ± 0.094 mm) and in the macular hole group (0.255 ± 0.111 mm) were significantly smaller than that in the healthy control group (0.358 ± 0.118 mm; all, P < 0.0001). Multiple regression analysis showed that a thicker fovea was significantly correlated with a smaller FAZ area in the epiretinal membrane group (r = -0.799, P < 0.0001), macular hole group (r = -0.473, P = 0.0042), and control group (r = -0.612, P < 0.0001). The FAZ area after internal limiting membrane peeling was smaller than that of the controls. A smaller FAZ area was correlated with a thicker fovea both in internal limiting membrane-peeled eyes and normal eyes.

Yakataga fold-and-thrust belt: Structural geometry and tectonic implications of a small continental collision zone

NASA Astrophysics Data System (ADS)

Wallace, Wesley K.

Collision of the Yakutat terrane with southern Alaska created a collisional fold-and-thrust belt along the Pacific-North America plate boundary. This southerner fold-and-thrust belt formed within continental sedimentary rocks but with the seaward vergence and tectonic position typical of an accretionary wedge. Northward exposure of progressively older rocks reflects that the fold-and-thrust belt forms a southward-tapered orogenic wedge that increases northward in structural relief and depth of erosion. Narrow, sharp anticlines separate wider, flat-bottomed synclines. Relatively steep thrust faults commonly cut the forelimbs of anticlines. Fold shortening and fault displacement both generally increase northward, whereas fault dip generally decreases northward. The coal-bearing lower part of the sedimentary section serves as a detachment for both folds and thrust faults. The folded and faulted sedimentary section defines a regional south dip of about 8°. The structural relief combined with the low magnitude of shortening of the sedimentary section suggest that the underlying basement is structurally thickened. I propose a new interpretation in which this thickening was accommodated by a passive-roof duplex with basement horses that are separated from the overlying folded and thrust-faulted sedimentary cover by a roof thrust with a backthrust sense of motion. Basement horses are ˜7 km thick, based on the thickness between the inferred roof thrust and the top of the basement in offshore seismic reflection data. This thickness is consistent with the depth of the zone of seismicity onshore. The inferred zone of detachment and imbrication of basement corresponds with the area of surface exposure of the fold-and-thrust belt within the Yakutat terrane and with the Wrangell subduction zone and arc farther landward. By contrast, to the west, the crust of the Yakutat terrane has been carried down a subduction zone that extends far landward with a gentle dip, corresponding

Measure Guideline: Basement Insulation Basics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aldrich, R.; Mantha, P.; Puttagunta, S.

2012-10-01

This guideline is intended to describe good practices for insulating basements in new and existing homes, and is intended to be a practical resources for building contractors, designers, and also to homeowners.

Barents Sea Paleozoic basement and basin configurations: Crustal structure from deep seismic and potential field data

NASA Astrophysics Data System (ADS)

Aarseth, Iselin; Mjelde, Rolf; Breivik, Asbjørn Johan; Huismans, Ritske; Faleide, Jan Inge

2016-04-01

The Barents Sea is underlain by at least two different basement domains; the Caledonian in the west and the Timanian in the east. The transition between these two domains is not well constrained and contrasting interpretations have been published recently. Interpretations of new high-quality magnetic data covering most of the SW Barents Sea has challenged the Late Paleozoic basin configurations in the western and central Barents Sea as outlined in previous studies. Two regional ocean bottom seismic (OBS) profiles were acquired in 2014. This new dataset crosses the two major directions of Caledonian deformation proposed by different authors: N-S direction and SW-NE direction. Of particular importance are the high velocity anomalies related to Caledonian eclogites, revealing the location of Caledonian suture zones in the northern Barents Sea. One of the main objectives with this project is to locate the main Caledonian suture in the western Barents Sea, as well as the possible Barentsia-Baltica suture postulated further eastwards. The collapse of the Caledonian mountain range predominantly along these suture zones is expected to be tightly linked to the deposition of large thicknesses of Devonian erosional products, and later rifting is expected to be influenced by inheritance of Caledonian trends. The P-wave travel-time modelling is done by use of a combined ray-tracing and inversion scheme, and gravity- and magnetic modelling will be used to augment the seismic model. The preliminary results indicate high P-wave velocities (mostly over 4 km/s) close to the seafloor as well as high velocity (around 6 km/s) zones at shallow depths which are interpreted as volcanic sills. The crustal transects reveal areas of complex geology and velocity inversions. A low seismic impedance contrast between the sedimentary section and top crystalline basement makes identification of this interface uncertain. Depth to Moho mostly lies around 30 km, except in an area of rapid change in

3. AERIAL VIEW, LOOKING SOUTH, OF BUILDING 371 BASEMENT UNDER ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. AERIAL VIEW, LOOKING SOUTH, OF BUILDING 371 BASEMENT UNDER CONSTRUCTION. THE BASEMENT HOUSES HEATING, VENTILATION, AND AIR CONDITIONING EQUIPMENT AND MECHANICAL UTILITIES, THE UPPER PART OF THE PLUTONIUM STORAGE VAULT AND MAINTENANCE BAY, AND SMALL PLUTONIUM PROCESSING AREAS. THE BASEMENT LEVEL IS DIVIDED INTO NEARLY EQUAL NORTH AND SOUTH PARTS BY THE UPPER PORTION OF THE PLUTONIUM STORAGE VAULT. (10/7/74) - Rocky Flats Plant, Plutonium Recovery Facility, Northwest portion of Rocky Flats Plant, Golden, Jefferson County, CO

Conceptual Model for Basement and Surface Structure Relationships in an Oblique Collision, Sawtooth Range, MT

NASA Astrophysics Data System (ADS)

Palu, J. M.; Burberry, C. M.

2014-12-01

The reactivation potential of pre-existing basement structures affects the geometry of subsequent deformation structures. A conceptual model depicting the results of these interactions can be applied to multiple fold-thrust systems and lead to valuable deformation predictions. These predictions include the potential for hydrocarbon traps or seismic risk in an actively deforming area. The Sawtooth Range, Montana, has been used as a study area. A model for the development of structures close to the Augusta Syncline in the Sawtooth Range is being developed using: 1) an ArcGIS map of the basement structures of the belt based on analysis of geophysical data indicating gravity anomalies and aeromagnetic lineations, seismic data indicating deformation structures, and well logs for establishing lithologies, previously collected by others and 2) an ArcGIS map of the surface deformation structures of the belt based on interpretation of remote sensing images and verification through the collection of surface field data indicating stress directions and age relationships, resulting in a conceptual model based on the understanding of the interaction of the two previous maps including statistical correlations of data and development of balanced cross-sections using Midland Valley's 2D/3D Move software. An analysis of the model will then indicate viable deformation paths where prominent basement structures influenced subsequently developed deformation structures and reactivated faults. Preliminary results indicate that the change in orientation of thrust faults observed in the Sawtooth Range, from a NNW-SSE orientation near the Gibson Reservoir to a WNW-ESE trend near Haystack Butte correlates with pre-existing deformation structures lying within the Great Falls Tectonic Zone. The Scapegoat-Bannatyne trend appears to be responsible for this orientation change and rather than being a single feature, may be composed of up to 4 NE-SW oriented basement strike-slip faults. This

Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype

PubMed Central

YANG, SHAOWEI; SUN, YEXIAO; GENG, ZHIJUN; MA, KUI; SUN, XIAOYAN; FU, XIAOBING

2016-01-01

The majority of studies on scar formation have mainly focused on the dermis and little is known of the involvement of the epidermis. Previous research has demonstrated that the scar tissue-derived keratinocytes are different from normal cells at both the genetic and cell biological levels; however, the mechanisms responsible for the fundamental abnormalities in keratinocytes during scar development remain elusive. For this purpose, in this study, we used normal, wound edge and hypertrophic scar tissue to examine the morphological changes which occur during epidermal regeneration as part of the wound healing process and found that the histological structure of hypertrophic scar tissues differed from that of normal skin, with a significant increase in epidermal thickness. Notably, staining of the basement membrane (BM) appeared to be absent in the scar tissues. Moreover, immunofluorescence staining for cytokeratin (CK)10, CK14, CK5, CK19 and integrin-β1 indicated the differential expression of cell markers in the epidermal keratinocytes among the normal, wound edge and hypertrophic scar tissues, which corresponded with the altered BM structures. By using a panel of proteins associated with BM components, we validated our hypothesis that the BM plays a significant role in regulating the cell fate decision of epidermal keratinocytes during skin wound healing. Alterations in the structure of the BM promote basal keratinocytes to adopt a proliferative phenotype both in vivo and in vitro. PMID:26986690

A possible source of water in seismogenic subduction zones

NASA Astrophysics Data System (ADS)

Kameda, J.; Yamaguchi, A.; Kimura, G.; Iodp Exp. 322 Scientists

2010-12-01

Recent works on the subduction megathrusts have emphasized the mechanical function of fluids contributing dynamic slip-weakening. Basalt-hosting fault zones in on-land accretionary complexes present several textures of seismic slip under fluid-assisted condition such as implosion breccia with carbonate matrix and decrepitation of fluid inclusion. In order to clarify initiation and evolution processes of such fault zones as well as possible source of fluid in the seismogenic subduction zone, we examined a mineralogical/geochemical feature of basaltic basement recovered by IODP Exp. 322 at C0012, that is a reference site for subduction input in the Nankai Trough. A total of 10 samples (about 4 m depth interval from the basement top) were analyzed in this study. XRD analyses indicate that all of the samples contain considerable amount of smectite. The smectite does not appear as a form of interstratified phase with illite or chlorite. Preliminary chemical analyses by EDS in TEM suggest that the smectite is trioctahedral saponite with Ca as a dominant interlayer cation. To determine the saponite content quantitatively, cation exchange capacity (CEC) of bulk samples was measured. The samples show almost similar CEC of around 30 meq/100g, implying that bulk rock contains about 30 wt% of saponite, considering a general CEC of 100 meq/100g for monomineralic saponite. Such abundance of saponite might be a result from intense alteration of oceanic crust due to sea water circulation at low temperature. Previous experimental work suggests that saponite might be highly hydrated (two to three water layer hydration form) at the seismogenic P-T condition. Hence, altered upper oceanic crust is a possible water sink in the seismogenic zone. The water stored in the smectite interlayer region will be expelled via smectite to chlorite transition reaction, that might contribute to the dynamic weakening of the seimogenic plate boundary between the basement basalt and overlying

Geochemical provenance of Florida basement components

DOE Office of Scientific and Technical Information (OSTI.GOV)

Heatherington, A.L.; Mueller, P.A.; Dallmeyer, R.D.

1993-03-01

The pre-Cretaceous basement of Florida is generally considered to be exotic with respect to Proterozoic Laurentia. Paleontologic and paleomagnetic evidence have suggested a Gondwanan provenance for the Floridan basement, as either a peri-Gondwanide terrane or as a rifted block of the West African craton. The report of generally similar lithologic sequences and a record of similar Ar-Ar cooling ages in some Floridan and West African lithologic units has led to very specific correlations between these units. U-Pb, Sm-Nd, and Rb-Sr geochronologic studies as well as isotopic and elemental abundance data have been used to evaluate the validity of these correlations.more » Results indicate: (1) geochemical similarities between volcanic rocks of northeastern Florida and a Pan-African metavolcanic sequence (Niokola-Koba group) exposed in Senegal; (2) an absence of a Grenvillian-age (i.e., Laurentian) component in zircons separated from a Paleozoic Suwanee basin sandstone; and (3) whole-rock Sm-Nd and U-Pb zircon evidence for an Archean ([approximately]3.0 Ga) component in the neo-Proterozoic Osceola granitoid(s). Although silicic rocks from throughout Florida have Nd model ages (T[sub DM]) that are predominantly Grenvillian (1.1--1.4 Ga), the absence of a Grenvillian component in zircons separated from granite and sandstone suggests that the model ages represent a mixture of older and younger components. Overall, the evidence for Birimian ([approximately]2.1 Ga) and Liberian ([approximately]3.0 Ga) age components in the Florida basement are consistent with its origin as a rifted block of cratonic Gondwana. In addition to demonstrating a strong affinity between the Florida basement and cratonic West Africa/northern South America, these data provide a basis for comparison with other circum-Atlantic terranes traditionally described as Avalonian/Cadomian, etc.« less

26. EXCAVATION OF EAST (FRONT) BASEMENT WELL AND DRAINAGE SYSTEM, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. EXCAVATION OF EAST (FRONT) BASEMENT WELL AND DRAINAGE SYSTEM, WITH ARCHED ENTRY INTO BASEMENT UNDER FRONT ENTRY IN BACKGROUND, LOOKING NORTH (NOTE GALLETING IN BRICK FOUNDATION) - Belair, Tulip Grove Drive, Belair-at-Bowie, Bowie, Prince George's County, MD

Hydrocarbons in New Guinea, controlled by basement fabric, Mesozoic extension and Tertiary convergent margin tectonics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hill, K.C.; Kendrick, R.D.; Crowhurst, P.V.

1996-01-01

Most models for the tectonic evolution of New Guinea involve Early and Late Miocene arc-continent collisions, creating an orogenic belt. Structural trends and prospectivity are then analyzed in terms of belts across the country; the Fold Belt (with the discovered oil and gas fields), the Mobile Belt and the accreted arcs. This model inhibits realistic assessment of prospectivity. It now appears the Mobile Belt formed by Oligocene compression then by Early Miocene extension, related to slab-rollback, that unroofed metamorphic core complexes adjacent to starved half-grabens. The grabens filled in the Middle Miocene and were largely transported intact during the Pliocenemore » arc-collision. Early Miocene reefs and hypothesized starved basin source rocks create a viable play throughout northern New Guinea as in the Salawati Basin. The Pliocene clastic section is locally prospective due to overthrusting and deep burial. Within the Fold Belt, the site and types of oil and gas fields are largely controlled by the basement architecture. This controlled the transfer zones and depocentres during Mesozoic extension and the location of major basement uplifts during compression. In PNG, the Bosavi lineament separates an oil province from a gas province. In Irian Jaya the transition from a relatively competent sequence to a rifted sequence west of [approx]139[degrees]E may also be a gas-oil province boundary. Understanding, in detail, the compartmentalization of inverted blocks and areas of thin-skinned thrusting, controlled by the basement architecture, will help constrain hydrocarbon prospectivity.« less

Hydrocarbons in New Guinea, controlled by basement fabric, Mesozoic extension and Tertiary convergent margin tectonics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hill, K.C.; Kendrick, R.D.; Crowhurst, P.V.

1996-12-31

Most models for the tectonic evolution of New Guinea involve Early and Late Miocene arc-continent collisions, creating an orogenic belt. Structural trends and prospectivity are then analyzed in terms of belts across the country; the Fold Belt (with the discovered oil and gas fields), the Mobile Belt and the accreted arcs. This model inhibits realistic assessment of prospectivity. It now appears the Mobile Belt formed by Oligocene compression then by Early Miocene extension, related to slab-rollback, that unroofed metamorphic core complexes adjacent to starved half-grabens. The grabens filled in the Middle Miocene and were largely transported intact during the Pliocenemore » arc-collision. Early Miocene reefs and hypothesized starved basin source rocks create a viable play throughout northern New Guinea as in the Salawati Basin. The Pliocene clastic section is locally prospective due to overthrusting and deep burial. Within the Fold Belt, the site and types of oil and gas fields are largely controlled by the basement architecture. This controlled the transfer zones and depocentres during Mesozoic extension and the location of major basement uplifts during compression. In PNG, the Bosavi lineament separates an oil province from a gas province. In Irian Jaya the transition from a relatively competent sequence to a rifted sequence west of {approx}139{degrees}E may also be a gas-oil province boundary. Understanding, in detail, the compartmentalization of inverted blocks and areas of thin-skinned thrusting, controlled by the basement architecture, will help constrain hydrocarbon prospectivity.« less

Geochemical, microtextural and petrological studies of the Samba prospect in the Zambian Copperbelt basement: a metamorphosed Palaeoproterozoic porphyry Cu deposit.

NASA Astrophysics Data System (ADS)

Master, Sharad; Mirrander Ndhlovu, N.

2015-04-01

Ever since Wakefield (1978, IMM Trans., B87, 43-52) described a porphyry-type meta-morphosed Cu prospect, the ca 50 Mt, 0.5% Cu Samba deposit (12.717°S, 27.833°E), hosted by porphyry-associated quartz-sericite-biotite schists in northern Zambia, there has been controversy about its origin and significance. This is because it is situated in the basement to the world's largest stratabound sediment-hosted copper province, the Central African Copperbelt, which is hosted by rocks of the Neoproterozoic Katanga Supergroup. Mineralization in the pre-Katangan basement has long played a prominent role in ore genetic models, with some authors suggesting that basement Cu mineralization may have been recycled into the Katangan basin through erosion and redeposition, while others have suggested that the circulation of fluids through Cu-rich basement may have leached out the metals which are found concentrated in the Katangan orebodies. On the basis of ca 490-460 Ma Ar-Ar ages, Hitzman et al. (2012, Sillitoe Vol., SEG Spec. Publ., 16, 487-514) suggested that Samba represents late-stage impregnation of copper mineralization into the basement, and that it was one of the youngest copper deposits known in the Central African Copperbelt. If the Samba deposit really is that young, then it would have post-dated regional deformation and metamorphism (560-510 Ma), and it ought to be undeformed and unmetamorphosed. The Samba mineralization consists of chalcopyrite and bornite, occurring as disseminations, stringers and veinlets, found in a zone >1 km along strike, in steeply-dipping lenses up to 10m thick and >150m deep. Our new major and trace element XRF geochemical data (14 samples) show that the host rocks are mainly calc-alkaline metadacites. Cu is correlated with Ag (Cu/Ag ~10,000:1) with no Au or Mo. Our study focused on the microtextures and petrology of the Samba ores. We confirm that there is alteration of similar style to that accompanying classical porphyry Cu mineralization

Bunkhouse basement interior showing storage area and a conveyor belt ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Bunkhouse basement interior showing storage area and a conveyor belt (circa 1936) used to unload dry goods into the basement through an opening on the east side of the bunkhouse. - Sespe Ranch, Bunkhouse, 2896 Telegraph Road, Fillmore, Ventura County, CA

Avoid the Wet Basement Blues: Construction Methods Guarantee Success

ERIC Educational Resources Information Center

Domermuth, David

2006-01-01

A damp basement can be a nightmare, especially after mildew creates its eye-watering, nose-offending stench. There are two enemies to overcome: damp air condensing on interior walls and ground water penetrating the exterior. One can address condensation by drying or heating the air in a basement to raise it above the dew point. The simple way to…

Quantitative analysis of external limiting membrane, ellipsoid zone and interdigitation zone defects in patients with macular holes.

PubMed

Houly, Jacques Ramos; Veloso, Carlos Eduardo; Passos, Elke; Nehemy, Márcio Bittar

2017-07-01

To investigate the correlation between the length of external limiting membrane (ELM), ellipsoid zone (EZ) and interdigitation zone (IZ) defects and visual prognosis in patients undergoing macular hole (MH) surgery, using spectral-domain optical coherence tomography (SD-OCT). This is a retrospective, consecutive, observational case series study. Fifty-two eyes of 52 patients with primary MH were evaluated. A quantitative analysis of ELM, EZ and IZ defects was performed preoperatively and at 3 and 6 months postoperatively using SD-OCT. The correlation between pre- and postoperative ELM, EZ and IZ defects and the best-corrected visual acuity (BCVA) was investigated. The lengths of ELM, EZ and IZ defects correlated significantly with BCVA in each study period (P < 0.001). Preoperative measures of these band defects were also associated with visual outcomes 3 and 6 months after surgery (P < 0.05). Considering all preoperative parameters, the length of the ELM defect was the factor most strongly correlated with BCVA at 6 months (β = 0.643, P < 0.012). The integrity of the ELM was the only factor significantly associated with BCVA at 6 months (β = 0.427; P = 0.004). The preoperative length of the ELM defect is the strongest predictor of visual acuity after MH surgery. Postoperative integrity of the ELM is significantly associated with visual restoration after surgical treatment of MH.

6. INTERIOR VIEW OF NORTH ENTRANCE TO BASEMENT SHOWING WORKBENCH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. INTERIOR VIEW OF NORTH ENTRANCE TO BASEMENT SHOWING WORKBENCH AT PHOTO LEFT AND ONE OF TWO DOORWAYS TO MAIN BASEMENT AREA AT PHOTO RIGHT. VIEW TO NORTH. - Bishop Creek Hydroelectric System, Control Station, Worker Cottage, Bishop Creek, Bishop, Inyo County, CA

Basement-involved faults and deep structures in the West Philippine Basin: constrains from gravity field

NASA Astrophysics Data System (ADS)

Wang, Gang; Jiang, Suhua; Li, Sanzhong; Zhang, Huixuan; Lei, Jianping; Gao, Song; Zhao, Feiyu

2017-06-01

To reveal the basement-involved faults and deep structures of the West Philippine Basin (WPB), the gravitational responses caused by these faults are observed and analyzed based on the latest spherical gravity model: WGM2012 Model. By mapping the free-air and Bouguer gravity anomalies, several main faults and some other linear structures are located and observed in the WPB. Then, by conducting a 2D discrete multi-scale wavelet decomposition, the Bouguer anomalies are decomposed into the first- to eighth-order detail and approximation fields (the first- to eighth-order Details and Approximations). The first- to third-order Details reflect detailed and localized geological information of the crust at different depths, and of which the higher-order reflects gravity field of the deeper depth. The first- to fourth-order Approximations represent the regional gravity fields at different depths of the crust, respectively. The fourth-order Approximation represents the regional gravity fluctuation caused by the density inhomogeneity of Moho interface. Therefore, taking the fourth-order Approximation as input, and adopting Parker-Oldenburg interactive inversion, We calculated the depth of Moho interface in the WPB. Results show that the Moho interface depth in the WPB ranges approximately from 8 to 12 km, indicating that there is typical oceanic crust in the basin. In the Urdaneta Plateau and the Benham Rise, the Moho interface depths are about 14 and 16 km, respectively, which provides a piece of evidence to support that the Banham Rise could be a transitional crust caused by a large igneous province. The second-order vertical derivative and the horizontal derivatives in direction 0° and 90° are computed based on the data of the third-order Detail, and most of the basement-involved faults and structures in the WPB, such as the Central Basin Fault Zone, the Gagua Ridge, the Luzon-Okinawa Fault Zone, and the Mindanao Fault Zone are interpreted by the gravity derivatives.

Microfluidic free-flow zone electrophoresis and isotachophoresis using carbon black nano-composite PDMS sidewall membranes.

PubMed

Fu, Xiaotong; Mavrogiannis, Nicholas; Ibo, Markela; Crivellari, Francesca; Gagnon, Zachary R

2017-01-01

We present a new type of free-flow electrophoresis (FFE) device for performing on-chip microfluidic isotachophoresis and zone electrophoresis. FFE is performed using metal gallium electrodes, which are isolated from a main microfluidic flow channel using thin micron-scale polydimethylsiloxane/carbon black (PDMS/CB) composite membranes integrated directly into the sidewalls of the microfluidic channel. The thin membrane allows for field penetration and effective electrophoresis, but serves to prevent bubble generation at the electrodes from electrolysis. We experimentally demonstrate the ability to use this platform to perform on-chip free-flow electrophoretic separation and isotachophoretic concentration. Due to the small size and simple fabrication procedure, this PDMS/CB platform could be used as a part of an on-chip upstream sample preparation toolkit for portable microfluidic diagnostic applications. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The Alteration of the Epidermal Basement Membrane Complex of Human Nevus Tissue and Keratinocyte Attachment after High Hydrostatic Pressurization.

PubMed

Morimoto, Naoki; Jinno, Chizuru; Mahara, Atsushi; Sakamoto, Michiharu; Kakudo, Natsuko; Inoie, Masukazu; Fujisato, Toshia; Suzuki, Shigehiko; Kusumoto, Kenji; Yamaoka, Tetsuji

2016-01-01

We previously reported that human nevus tissue was inactivated after high hydrostatic pressure (HHP) higher than 200 MPa and that human cultured epidermis (hCE) engrafted on the pressurized nevus at 200 MPa but not at 1000 MPa. In this study, we explore the changes to the epidermal basement membrane in detail and elucidate the cause of the difference in hCE engraftment. Nevus specimens of 8 mm in diameter were divided into five groups (control and 100, 200, 500, and 1000 MPa). Immediately after HHP, immunohistochemical staining was performed to detect the presence of laminin-332 and type VII collagen, and the specimens were observed by transmission electron microscopy (TEM). hCE was placed on the pressurized nevus specimens in the 200, 500, and 1000 MPa groups and implanted into the subcutis of nude mice; the specimens were harvested at 14 days after implantation. Then, human keratinocytes were seeded on the pressurized nevus and the attachment was evaluated. The immunohistochemical staining results revealed that the control and 100 MPa, 200 MPa, and 500 MPa groups were positive for type VII collagen and laminin-332 immediately after HHP. TEM showed that, in all of the groups, the lamina densa existed; however, anchoring fibrils were not clearly observed in the 500 or 1000 MPa groups. Although the hCE took in the 200 and 500 MPa groups, keratinocyte attachment was only confirmed in the 200 MPa group. This result indicates that HHP at 200 MPa is preferable for inactivating nevus tissue to allow its reuse for skin reconstruction in the clinical setting.

Anti-nucleosome antibodies complexed to nucleosomal antigens show anti-DNA reactivity and bind to rat glomerular basement membrane in vivo.

PubMed Central

Kramers, C; Hylkema, M N; van Bruggen, M C; van de Lagemaat, R; Dijkman, H B; Assmann, K J; Smeenk, R J; Berden, J H

1994-01-01

Histones can mediate the binding of DNA and anti-DNA to the glomerular basement membrane (GBM). In ELISA histone/DNA/anti-DNA complexes are able to bind to heparan sulfate (HS), an intrinsic constituent of the GBM. We questioned whether histone containing immune complexes are able to bind to the GBM, and if so, whether the ligand in the GBM is HS. Monoclonal antibodies (mAbs) complexed to nucleosomal antigens and noncomplexed mAbs were isolated from culture supernatants of four IgG anti-nuclear mAbs. All noncomplexed mAbs showed strong anti-nucleosome reactivity in ELISA. One of them showed in addition anti-DNA reactivity in noncomplexed form. The other three mAbs only showed anti-DNA reactivity when they were complexed to nucleosomal antigens. After renal perfusion a fine granular binding of complexed mAbs to the glomerular capillary wall and activation of complement was observed in immunofluorescence, whereas noncomplexed mAbs did not bind. Immuno-electron microscopy showed binding of complexes to the whole width of the GBM. When HS in the GBM was removed by renal heparinase perfusion the binding of complexed mAb decreased, but did not disappear completely. We conclude that anti-nucleosome mAbs, which do not bind DNA, become DNA reactive once complexed to nucleosomal antigens. These complexed mAbs can bind to the GBM. The binding ligand in the GBM is partly, but not solely, HS. Binding to the GBM of immune complexes containing nucleosomal material might be an important event in the pathogenesis of lupus nephritis. Images PMID:8040312

Poliomyelitis in MuLV-infected ICR-SCID mice after injection of basement membrane matrix contaminated with lactate dehydrogenase-elevating virus.

PubMed

Carlson Scholz, Jodi A; Garg, Rohit; Compton, Susan R; Allore, Heather G; Zeiss, Caroline J; Uchio, Edward M

2011-10-01

The arterivirus lactate dehydrogenase-elevating virus (LDV) causes life-long viremia in mice. Although LDV infection generally does not cause disease, infected mice that are homozygous for the Fv1(n) allele are prone to develop poliomyelitis when immunosuppressed, a condition known as age-dependent poliomyelitis. The development of age-dependent poliomyelitis requires coinfection with endogenous murine leukemia virus. Even though LDV is a common contaminant of transplantable tumors, clinical signs of poliomyelitis after inadvertent exposure to LDV have not been described in recent literature. In addition, LDV-induced poliomyelitis has not been reported in SCID or ICR mice. Here we describe the occurrence of poliomyelitis in ICR-SCID mice resulting from injection of LDV-contaminated basement membrane matrix. After exposure to LDV, a subset of mice presented with clinical signs including paresis, which was associated with atrophy of the hindlimb musculature, and tachypnea; in addition, some mice died suddenly with or without premonitory signs. Mice presenting within the first 6 mo after infection had regions of spongiosis, neuronal necrosis and astrocytosis of the ventral spinal cord, and less commonly, brainstem. Axonal degeneration of ventral roots prevailed in more chronically infected mice. LDV was identified by RT-PCR in 12 of 15 mice with typical neuropathology; positive antiLDV immunolabeling was identified in all PCR-positive animals (n = 7) tested. Three of 8 mice with neuropathology but no clinical signs were LDV negative by RT-PCR. RT-PCR yielded murine leukemia virus in spinal cords of all mice tested, regardless of clinical presentation or neuropathology.

The Subduction of an Exhumed and Serpentinized Magma-Poor Basement Beneath the Northern Lesser Antilles Reveals the Early Tectonic Fabric at Slow-Spreading Mid-Oceanic Ridges

NASA Astrophysics Data System (ADS)

Marcaillou, B.; Klingelhoefer, F.; Laurencin, M.; Biari, Y.; Graindorge, D.; Jean-Frederic, L.; Laigle, M.; Lallemand, S.

2017-12-01

Multichannel and wide-angle seismic data as well as heat-flow measurements (ANTITHESIS cruise, 2016) reveal a 200x200km patch of magma-poor oceanic basement in the trench and beneath the outer fore-arc offshore of Antigua to Saint Martin in the Northern Lesser Antilles. These data highlight an oceanic basement with the following features: 1/ Absence of any reflection at typical Moho depth and layer2/layer3 limit depths. 2/ High Velocity Vp at the top (>5.5 km/s), low velocity gradient with depth (<0.3 s-1) and no significant velocity change at theoretical Moho depth. 3/ Anomalously low heat-flow (40±15mW.m-2) compared to the central Antilles and to theoretical values for an 80 Myr-old oceanic plate suggesting the influence of deep hydrothermal circulation. 4/ Two sets of reflections dipping toward the paleo mid-Atlantic ridge and toward the Vidal Transform Fault Zone respectively. These highly reflective planes sometimes fracture the top of the basement, deforming the interplate contact and extend downward to 20km depth with a 20° angle. We thus propose that a large patch of mantle rocks, exhumed and serpentinized at the slow-spreading mid-Atlantic Ridge 80 Myr ago, is currently subducting beneath the Northern Lesser Antilles. During the exhumation, early extension triggers penetrative shear zones sub-parallel to the ridge and to the transform fault. Eventually, this early extension generates sliding along the so-called detachment fault, while the other proto-detachment abort. Approaching the trench, the plate bending reactivates these weak zones in normal faults and fluid pathways promoting deep serpentinisation and localizing tectonic deformation at the plate interface. These subducting fluid-rich mechanically weak mantle rocks rise questions about their relation to the faster slab deepening, the lower seismic activity and the pervasive tectonic partitioning in this margin segment.

Reactivation versus reworking of the active continental margin during the Zagros collision: Mahallat-Muteh-Laybid complexes, Sanandaj-Sirjan zone, Iran

NASA Astrophysics Data System (ADS)

Aflaki, Mahtab; Shabanian, Esmaeil; Davoodi, Zeinab; Mohajjel, Mohammad

2017-06-01

Reactivation of long-lived basement faults has significant influences on further deformation of collision zones. Three major inherited pre-collisional NW-, N- and NE-trending basement discontinuities have played important roles on the structural and tectono-sedimentary evolution of the Iranian micro-continent in the northeastern part of the Gondwana super-continent. Sanandaj-Sirjan zone (SSZ), known as the metamorphic belt of the Zagros orogeny, marks the SW margin of the Central Iran. SSZ is formed as a result of the Arabia-Eurasia collision and its general trend of deformation coincides with the NW structural trend of the collision. The NE-trending Mahallat, Muteh and Laybid complexes in the middle part of the NW-trending SSZ are the exception and have a trend almost normal to the NW-trending Zagros. A combined methodology of remote sensing, geometric and kinematics analyses complemented by field work was used to reconstruct the history of deformation in the Zagros hinterland since the earlier stages of collision to the present-day. Our results reveal the key role of the preexisting discontinuities of the Iranian basement in both the kinematics and structural pattern of the middle part of the SSZ. These basement faults have acted as main boundary conditions changing the collisional fabric perpendicular to its overall trend. Progressive deformation and the related changes during collision have caused drastic changes in the kinematics of the boundary faults. The establishment of dextral transtension in the SSZ has had secondary influences on the pattern of deformation by local clockwise rotation and localized dextral shear in the southern parts of the area of interest. This study highlights the significance of long-lived pre-existing structures in the deformation of collision zones. Such basement faults are capable to change both the pattern and kinematics of deformation of the adjacent areas involved in a continental collision.

Interdigitation Zone Band Restoration After Treatment of Diabetic Macular Edema.

PubMed

Serizawa, Satoshi; Ohkoshi, Kishiko; Minowa, Yuko; Soejima, Kumiko

2016-09-01

To investigate whether the integrity of the interdigitation zone band, the ellipsoid zone band, and the external limiting membrane are reliable markers of treatment outcome in diabetic macular edema (DME). In this retrospective study, we examined 41 treatment-naïve eyes (38 patients) with DME that were treated with laser therapy, pharmacotherapy, and/or vitrectomy. Best-corrected visual acuity and the integrity of the interdigitation zone band, the ellipsoid zone band, and the external limiting membrane were assessed before treatment and at 3, 6, and 12 months after DME treatment. One year after treatment, the external limiting membrane, ellipsoid zone band, and interdigitation zone band were completely visible in 30 (73.2%), 24 (58.5%), and 2 (4.9%) eyes, respectively. Interdigitation zone band status improved significantly (P = 0.005) 1 year after treatment. The interdigitation zone did not improve in the absence of the ellipsoid zone band. Likewise, ellipsoid zone status did not improve in the absence of the external limiting membrane at any time after treatment. The results of this study show that restoration of the interdigitation zone band constitutes a very sensitive marker of DME treatment outcome when the ellipsoid zone band is visible before treatment.

The morphology and nature of the East Arctic ocean acoustic basement

NASA Astrophysics Data System (ADS)

Rekant, Pavel

2017-04-01

As the result of the thorough interpretation and cross-correlation of the large seismic dataset (>150000 km and >600 seismic lines), the depth structure map of the acoustic basement was constrained. Tectonic framework, basement surface morphology and linkage of the deep basin structures with shelves ones, was significantly clarified based on the map. It becomes clear that most morphostructures presently located within deep-water basin are tectonically connected with shelf structures. Acoustic basement contains a number of pre-Cambrian, Caledonian and Mesozoic consolidated blocks. The basement heterogeneity is highlighted by faults framework and basement surface morphology differences, as well thickness and stratigraphy of the sediment cover. The deepest basins of the East Arctic - Hanna Trough, North Chukchi and Podvodnikov Basins form a united mega-depression, wedged between pre-Cambrian continental blocks (Chukchi Borderland - Mendeleev Rise - Toll Saddle) from the north and the Caledonian deformation front from the south. The basement age/origin speculations are consistent with paleontological and U-Pb zircon ages from dredged rock samples. Most of morphological boundaries in the modern Arctic differ considerably from the tectonic framework. Only part of the Arctic morphostructures is constrained by tectonic boundaries. They are: eastern slope of the Lomonosov Ridge, continental slope in the Laptev Sea, upper continental slope in the Podvodnikov Basin, southern slope of the North Chukchi Basin and borders of the Chukchi Borderland. The rest significant part of modern morphological boundaries are caused by sedimentation processes.

Deformation associated with the Ste. Genevieve fault zone and mid-continent tectonics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schultz, A.; Baker, G.S.; Harrison, R.W.

1992-01-01

The Ste. Genevieve fault is a northwest-trending deformation zone on the northeast edge of the Ozark Dome in Missouri. The fault has been described as a high-angle block fault resulting from vertical uplift of Proterozoic basement rocks, and also as a left-lateral, strike-slip or transpressive wrench fault associated with the Reelfoot rift. Recent mapping across the fault zone documents significant changes in the style of deformation along strike, including variations in the number and the spacing of fault strands, changes in the orientation of rocks within and adjacent to the fault zone, and changes in the direction of stratigraphic offsetmore » between different fault slices. These data are inconsistent with existing Ste. Genevieve models of monoclinal folding over basement upthrusts. Mesoscopic structural analysis of rocks in and near the fault zone indicates highly deformed noncylindrical folds, faults with normal, reverse, oblique, and strike-slip components of movement, and complex joint systems. Fabric orientation, calcite shear fibers, and slickensides indicate that the majority of these mesoscopic structures are kinematically related to left-lateral oblique slip with the southwest side up. Within the fault zone are highly fractured rocks, microscopic to coarse-grained carbonate breccia, and siliciclastic cataclasite. Microscopic deformation includes twinning in carbonate rocks, deformation banding, undulose extinction, and strain-induced polygonization in quartz, tectonic stylolites, extension veining, microfractures, and grain-scale cataclasis. Data are consistent with models relating the Ste. Genevieve fault zone to left-lateral oblique slip possibly associated with New Madrid tectonism.« less

U redox fronts and kaolinisation in basement-hosted unconformity-related U ores of the Athabasca Basin (Canada): late U remobilisation by meteoric fluids

NASA Astrophysics Data System (ADS)

Mercadier, Julien; Cuney, Michel; Cathelineau, Michel; Lacorde, Mathieu

2011-02-01

Proterozoic basement-hosted unconformity-related uranium deposits of the Athabasca Basin (Saskatchewan, Canada) were affected by significant uranium redistribution along oxidation-reduction redox fronts related to cold and late meteoric fluid infiltration. These redox fronts exhibit the same mineralogical and geochemical features as the well-studied uranium roll-front deposits in siliclastic rocks. The primary hydrothermal uranium mineralisation (1.6-1.3 Ga) of basement-hosted deposits is strongly reworked to new disseminated ores comprising three distinctly coloured zones: a white-green zone corresponding to the previous clay-rich alteration halo contemporaneous with hydrothermal ores, a uranium front corresponding to the uranium deposition zone of the redox front (brownish zone, rich in goethite) and a hematite-rich red zone marking the front progression. The three zones directly reflect the mineralogical zonation related to uranium oxides (pitchblende), sulphides, iron minerals (hematite and goethite) and alumino-phosphate-sulphate (APS) minerals. The zoning can be explained by processes of dissolution-precipitation along a redox interface and was produced by the infiltration of cold (<50°C) meteoric fluids to the hydrothermally altered areas. U, Fe, Ca, Pb, S, REE, V, Y, W, Mo and Se were the main mobile elements in this process, and their distribution within the three zones was, for most of them, directly dependent on their redox potential. The elements concentrated in the redox fronts were sourced by the alteration of previously crystallised hydrothermal minerals, such as uranium oxides and light rare earth element (LREE)-rich APS. The uranium oxides from the redox front are characterised by LREE-enriched patterns, which differ from those of unconformity-related ores and clearly demonstrate their distinct conditions of formation. Uranium redox front formation is thought to be linked to fluid circulation episodes initiated during the 400-300 Ma period during

Membranous glomerulonephritis in a child asymptomatic for hepatitis B virus. Concomitant seropositivity for HBsAG and anti-HBs.

PubMed

Hirsch, H Z; Ainsworth, S K; DeBeukelaer, M; Brissie, R M; Hennigar, G R

1981-04-01

The presence of hepatitis B surface antigen (HBsAg) in association with immunoglobulins and complement components within the glomerular basement membranes of adults having chronic active hepatitis has been well documented. In addition, investigators in Poland have demonstrated HBsAg immune complexes in glomeruli of children who did not have clinical evidence of hepatitis. More recently, a single case of childhood membranous glomerulonephritis in an asymptomatic carrier of hepatitis B virus was cited by observers in Canada. Reported here is the deposition of HBsAg immune complexes in the glomerular basement membranes of a 13-year-old black boy who had membranous glomerulopathy but not clinical evidence of hepatitis. This may be the first reported case in the United States of HbsAg-associated membranous glomerulonephritis in a child asymptomatic for hepatitis B virus, and only the second such case in North America. However, unlike previous studies of childhood glomerulopathy in association with hepatitis B virus, this patient is seropositive for both HBsAg and anti-HBs (antibody for hepatitis B surface antigen). Similar "rare" serologic findings were found for the patient's eldest male sib.

Evidence for synchronous thin-skinned and basement deformation in the Cordilleran fold-thrust belt: the Tendoy Mountains, southwestern Montana

NASA Astrophysics Data System (ADS)

McDowell, Robin John

1997-01-01

The Tendoy Mountains contain the easternmost thin-skinned thrust sheets in the Cordilleran fold-thrust belt of southwestern Montana, and are in the zone of tectonic overlap between the Rocky Mountain foreland and the Cordilleran fold-thrust belt. The three frontal thrust sheets of the Tendoy Mountains are from north to south, the Armstead, McKenzie, and Tendoy sheets. Near the southeastern terminus of the Tendoy thrust sheet is a lateral ramp in which the Tendoy thrust climbs along strike from the Upper Mississippian Lombard Limestone to lower Cretaceous rocks. This ramp coincides with the southeastern side of the Paleozoic Snowcrest trough and projection of the range-flanking basement thrust of the Blacktail-Snowcrest uplift, suggesting either basement or stratigraphic control on location of the lateral ramp. Axes of major folds on the southern part of the Tendoy thrust sheet are parallel to the direction of thrust transport and to the trend of the Snowcrest Range. They are a result of: (1) Pre-thrust folding above basement faults; (2) Passive transportation of the folds from a down-plunge position; (3) Minor reactivation of basement faults; and (4) Emplacement of blind, sub-Tendoy, thin-skinned thrust faults. The Tendoy sheet also contains a major out-of-sequence thrust fault that formed in thick Upper Mississippian shales and created large, overturned, foreland-verging folds in Upper Mississippian to Triassic rocks. The out-of-sequence fault can be identified where stratigraphic section is omitted, and by a stratigraphic separation diagram that shows it cutting down section in the direction of transport. The prominent lateral ramp at the southern terminus of the Tendoy thrust sheet is a result of fault propagation through strata folded over the edge of the Blacktail-Snowcrest uplift.

Radiolytic hydrogen production in basaltic basement of the South Pacific Gyre

NASA Astrophysics Data System (ADS)

Dzaugis, M. E.; Spivack, A. J.; Dunlea, A. G.; Murray, R. W.; Kelley, K. A.; D'Hondt, S. L.

2013-12-01

Water radiolysis is the decomposition of water molecules due to interactions with ionizing radiation from the natural decay of radioactive elements, such as uranium (238U, 235U), thorium (232Th) and potassium (40K). This abiotic process produces electron donors (e.g., H2) and acceptors (e.g., O2) that microorganisms can metabolize for energy. Although water radiolysis has been examined in deep continental crust (Lin et al., 2005) and marine sediment (Blair et al., 2007), it has not been rigorously addressed in oceanic basement. The submarine depth to which life extends on Earth, and the potential for life in basaltic aquifers of other worlds (such as Mars and Europa), may depend on radiolytic production of electron donors and acceptors. In order to quantify the extent to which water radiolysis occurs in the subseafloor basaltic basement, we (i) quantified radioactive element concentrations of basement samples from Integrated Ocean Drilling Program (IODP) Expedition 329 and (ii) developed a quantitative model of H2 production by water radiolysis in the basement aquifer. Modeling radiolytic production of H2 in oceanic basement is difficult because the basement is a heterogeneous environment. Microscale changes in physical properties and chemical composition cause microscale variation in water radiolysis within the basement. During radioactive decay, alpha particles, beta particles and gamma rays are emitted, each with a spectrum of characteristic energies. The distance over which radiation is attenuated depends on the kind of radiation (alpha, beta or gamma), initial energy, and the absorbing material. These properties and the concentration of radioactive elements provide the basis for our preliminary model. We are using inductively coupled plasma emission spectroscopy (ICP-ES), mass spectrometry (ICP-MS) and laser ablation (LA ICP-MS) to map variation in radioelement concentrations from phase to phase (e.g., across successive alteration halos to unaltered rock). The

Ivory Basements and Ivory Towers

ERIC Educational Resources Information Center

Fitzgerald, Tanya

2012-01-01

The metaphors of the ivory tower and ivory basement are used in this chapter to reflect how many women understand and experience the academy. The ivory tower signifies a place that is protected, a place of privilege and authority and a place removed from the outside world (and consequently the rigours of the market place). The ivory tower, by…

Oxygen activation at the plasma membrane: relation between superoxide and hydroxyl radical production by isolated membranes.

PubMed

Heyno, Eiri; Mary, Véronique; Schopfer, Peter; Krieger-Liszkay, Anja

2011-07-01

Production of reactive oxygen species (hydroxyl radicals, superoxide radicals and hydrogen peroxide) was studied using EPR spin-trapping techniques and specific dyes in isolated plasma membranes from the growing and the non-growing zones of hypocotyls and roots of etiolated soybean seedlings as well as coleoptiles and roots of etiolated maize seedlings. NAD(P)H mediated the production of superoxide in all plasma membrane samples. Hydroxyl radicals were only produced by the membranes of the hypocotyl growing zone when a Fenton catalyst (FeEDTA) was present. By contrast, in membranes from other parts of the seedlings a low rate of spontaneous hydroxyl radical formation was observed due to the presence of small amounts of tightly bound peroxidase. It is concluded that apoplastic hydroxyl radical generation depends fully, or for the most part, on peroxidase localized in the cell wall. In soybean plasma membranes from the growing zone of the hypocotyl pharmacological tests showed that the superoxide production could potentially be attributed to the action of at least two enzymes, an NADPH oxidase and, in the presence of menadione, a quinone reductase.

Dialyzer-related Thrombocytopenia due to a Polysulfone Membrane.

PubMed

Kobari, Eri; Terawaki, Hiroyuki; Takahashi, Yasuhito; Kusano, Yuki; Sakurai, Kaoru; Matsunaga, Keiko; Fukushima, Naotaro; Suzuki, Sawako; Tanaka, Ken-Ichi; Hayashi, Yoshimitsu; Watanabe, Tsuyoshi; Nakayama, Masaaki

2016-01-01

A 72-year-old Japanese woman was admitted to our hospital with rapidly progressive glomerulonephritis associated with anti-glomerular basement membrane antibody. Hemodialysis (HD) therapy was initiated on the day of admission using a biocompatible polysulfone (PS) membrane. Her platelet count (PLT; ×10(4)/μL) decreased gradually from 58.7 (day 1) to 5.8 (day 25). Considering the possibility of dialyzer-related thrombocytopenia (DRT), we measured her PLT count before and after the HD session on day 72, which revealed a dramatic decrease of 7.5 to 4.3. This finding suggested that the PS dialyzer caused PLT depletion. After discontinuation of the PS dialyzer, DRT was resolved.

Cover sequence stratigraphy and structure: Salem Church basement culmination, Georgia Blue Ridge

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, L.; Tull, J.F.

The Salem Church anticline SW of Jasper, Georgia in the western Blue Ridge is roughly an oval shaped structural dome with its long axis trending NE-SW. The anticline is cored by the Grenville age Corbin Gneiss which represents allochthonous North American basement. In debate for decades has been the age and origin of several kilometers of poly-deformed cover sequence rocks which were metamorphosed to greenschist facies and were probably transported over a long distance inland after their deposition. The stratigraphy of the cover sequence exhibits rapid lithofacies changes. At most localities, the basement is overlain by a 500--600 m thickmore » coarse clastic unit sourced from the basement rocks, composed mainly of metaconglomerate, metasandstone and metadiamictite. A thin unit less than 20 m thick of sericite phyllite occurs between the basement and the coarse clastic unit along the SE limb of the anticline but pinches out to the MW. A relatively sharp stratigraphic contact occurs between quartzite unit and overlying dark colored metagreywackes and metadiamictites containing distinctive cobbles and boulders of granitic and gneissic basement rocks up to 1 meter in length. This unit is about 100 m thick in the SW but thins rapidly towards the NE. It grades up into a geographically widespread graphitic phyllite which encircles most of the anticline. Unlike the cover sequence above the corbin basement west of Waleska, Georgia, no carbonate is found in this area.« less

The Salton Seismic Imaging Project: Seismic velocity structure of the Brawley Seismic Zone, Salton Buttes and Geothermal Field, Salton Trough, California

NASA Astrophysics Data System (ADS)

Delph, J.; Hole, J. A.; Fuis, G. S.; Stock, J. M.; Rymer, M. J.

2011-12-01

The Salton Trough is an active rift in southern California in a step-over between the plate-bounding Imperial and San Andreas Faults. In March 2011, the Salton Seismic Imaging Project (SSIP) investigated the rift's crustal structure by acquiring several seismic refraction and reflection lines. One of the densely sampled refraction lines crosses the northern-most Imperial Valley, perpendicular to the strike-slip faults and parallel to a line of small Quaternary rhyolitic volcanoes. The line crosses the obliquely extensional Brawley Seismic Zone and goes through one of the most geothermally productive areas in the United States. Well logs indicate the valley is filled by several kilometers of late Pliocene-recent lacustrine, fluvial, and shallow marine sediment. The 42-km long seismic line was comprised of eleven 110-460 kg explosive shots and receivers at a 100 m spacing. First arrival travel times were used to build a tomographic seismic velocity image of the upper crust. Velocity in the valley increases smoothly from <2 km/s to >5 km/s, indicating diagenesis and gradational metamorphism of rift sediments at very shallow depth due to an elevated geotherm. The velocity gradient is much smaller in the relatively low velocity (<6 km/s) crystalline basement comprised of recently metamorphosed sediment reaching greenschist to lower amphibolite facies. The depth of this basement is about 4-km below the aseismic region of the valley west of the Brawley Seismic Zone, but rises sharply to ~2 km depth beneath the seismically, geothermally, and volcanically active area of the Brawley Seismic Zone. The basement deepens to the northeast of the active tectonic zone and then is abruptly offset to shallower depth on the northeast side of the valley. This offset may be the subsurficial expression of a paleofault, most likely an extension of the Sand Hills Fault, which bounds the basin to the east. Basement velocity east of the fault is ~5.7 km/s, consistent with the granitic rocks

Transient formation fluid pressures and temperatures in the Costa Rica forearc prism and subducting oceanic basement: CORK monitoring at ODP Sites 1253 and 1255

NASA Astrophysics Data System (ADS)

Davis, Earl E.; Villinger, Heinrich W.

2006-05-01

Seafloor and formation-fluid pressure data from two Ocean Drilling Program (ODP) borehole hydrologic observatories installed at the toe of the subduction-zone prism off Costa Rica provide new information about the average and transient state of this non-accretionary prism. Data collected to date span a 16-month period from the time of installation during ODP Leg 205 in late 2002 to the most recent submersible site visit in March 2004. Pressure monitoring is part of a larger coordinated effort involving temperature monitoring and continuous fluid sampling within the formation and at the seafloor. The holes are positioned 800 m apart and monitoring points include two in igneous basement just seaward of the prism toe, one in the decollement that separates the underthrust sediments of the incoming Cocos plate from the Costa Rica prism, and one in the overthrust-prism sediments. Response of formation-fluid pressure to oceanographic loading at the seafloor constrains the framework compressibility of basement (ca . 1.1-1.3 × 10 - 10 Pa - 1) and the prism and decollement sediments (ca . 4-7 × 10 - 9 Pa - 1). Values are equivalent to ones determined elsewhere in similar sections. Once effects of seafloor loading are removed, pressures at both basement levels are seen to be steady, nearly identical, and less than but very close to hydrostatic (- 6 kPa). This state probably reflects the local hydrothermal regime of the oceanic crust, not the hydrologic regime of the consolidating subduction complex, and is consistent with basement being highly permeable and hydrologically well connected to distant igneous outcrops where free exchange of water between the crust and the ocean can occur. To what depth in the subduction zone high basement permeability persists is not known, but until permeability is reduced by alteration or mechanical fracture closure, basement must serve to provide a drainage path for water expelled from the consolidating underthrust sedimentary section. The

Membrane events in the acrosomal reaction of Limulus sperm. Membrane fusion, filament-membrane particle attachment, and the source and formation of new membrane surface

PubMed Central

1979-01-01

The membranes of Limulus (horseshoe crab) sperm were examined before and during the acrosomal reaction by using the technique of freeze- fracturing and thin sectioning. We focused on three areas. First, we examined stages in the fusion of the acrosomal vacuole with the cell surface. Fusion takes place in a particle-free zone which is surrounded by a circlet of particles on the P face of the plasma membrane and an underlying circlet of particles on the P face of the acrosomal vauole membrane. These circlets of particles are present before induction. Up to nine focal points of fusion occur within the particle-free zone. Second, we describe a system of fine filaments, each 30 A in diameter, which lies between the acrosomal vacuole and the plasma membrane. These filaments change their orientation as the vacuole opens, a process that takes place in less than 50 ms. Membrane particles seen on the P face of the acrosomal vacuole membrane change their orientation at the same time and in the same way as do the filaments, thus indicating that the membrane particles and filaments are probably connected. Third, we examined the source and the point of fusion of new membrane needed to cover the acrosomal process. This new membrane is almost certainly derived from the outer nuclear envelope and appears to insert into the plasma membrane in a particle-free area adjacent to an area rich in particles. The latter is the region where the particles are probably connected to the cytoplasmic filaments. The relevance of these observations in relation to the process of fertilization of this fantastic sperm is discussed. PMID:582596

27. EXCAVATION OF EAST (FRONT) BASEMENT WELL AND DRAINAGE SYSTEM, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. EXCAVATION OF EAST (FRONT) BASEMENT WELL AND DRAINAGE SYSTEM, WITH ARCHED ENTRY INTO BASEMENT UNDER FRONT ENTRY IN BACKGROUND, LOOKING NORTH (NOTE GALLETING IN BRICK FOUNDATION) BUT CLOSER RANGE SHOWING BRICK STRUCTURE WHICH CARRIED WATER FROM THE GUTTER DRAIN PIPE INTO THE BRICK DRAIN ALONG THE GROUND AND AWAY FROM THE FOUNDATION OF THE HOUSE - Belair, Tulip Grove Drive, Belair-at-Bowie, Bowie, Prince George's County, MD

22. VIEW OF THE BASEMENT AND MEZZANINE FLOOR PLANS. ALL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. VIEW OF THE BASEMENT AND MEZZANINE FLOOR PLANS. ALL MECHANICAL AND ELECTRICAL UTILITY EQUIPMENT IS CONTAINED IN THE BASEMENT. THE MEZZANINE CONTAINS OFFICES. THE DRAWING WAS REPRODUCED AT THE BEST QUALITY POSSIBLE. LETTERS AND NUMBERS IN THE CIRCLES INDICATE FOOTER AND/OR COLUMN LOCATIONS. - Rocky Flats Plant, Non-Nuclear Production Facility, South of Cottonwood Avenue, west of Seventh Avenue & east of Building 460, Golden, Jefferson County, CO

Volcanism, mantle exhumation and spreading at the axial zone of a fossil slow spreading ocean

NASA Astrophysics Data System (ADS)

Chalot-Prat, F.; Coco, E.

2003-04-01

Within an axial zone of a slow spreading ocean, the mechanisms checking together volcano emplacement, mantle exhumation and ocean enlargement are poorly known. In order to better assess how they could be linked , a detailed mapping of a fossil ocean-floor structure, preserved from alpine tectonic and metamorphism, was performed in the Chenaillet unit (Franco-Italian Alps)(Chalot-Prat &Coco, submit.). The detailed 3D geometry of the ophiolite evidences that from its dimensions, topography, morphology, and the architecture of the volcanic cover at different scales, the Chenaillet unit is a witness of an axial zone of Atlantic type. The basement (serpentinized peridotites and gabbros), below and in the prolongation of the volcanic cover (le50 m), is capped by a tectonic breccias horizon (Chalot-Prat and Manatschal, 2002), underlining detachment faults responsible for its exhumation at the seafloor. Clasts of dolerite, found within the fault zone, indicate that basement exhumation had to be active during and even after volcano emplacement. Stair- and comb-type volcanic systems check the distribution of individual volcanoes; the higher the edifice, the younger it is relative to the others. In the stair-type (up to 600 m of height difference between base and top), each step is formed with a pillow and tube tongue stacking fed from fissural conduits located at the root of each step. This system formed by uplift, step by step fracturation of an already exhumed basement, and magma injection along the fissures once formed. The comb-type (up to 200 m of height difference between base and top) consists in well-defined alignments of pillow and tube conic edifices. Their central feeder dykes are emplaced on the crossing of two types of fractures, oblique (tooth) and parallel (line) to the main branch of the comb. Along a same line, eruptions are coeval as proved by rhythmic variations of major and trace element contents of basalts from one line to another. The comb formation

Possible detachment zone in Precambrian rocks of Kanjamalai Hills, Cauvery Suture Zone, Southern India: Implications to accretionary tectonics

NASA Astrophysics Data System (ADS)

Mohanty, D. P.; Chetty, T. R. K.

2014-07-01

Existence of a possible detachment zone at Elampillai region, NW margin of Kanjamalai Hills, located in the northern part of Cauvery Suture Zone (CSZ), Southern India, is reported here for the first time. Detailed structural mapping provides anatomy of the zone, which are rarely preserved in Precambrian high grade terranes. The detachment surface separates two distinct rock units of contrasting lithological and structural characters: the upper and lower units. The detachment zone is characterized by a variety of fold styles with the predominance of tight isoclinal folds with varied plunge directions, limb rotations and the hinge line variations often leading to lift-off fold like geometries and deformed sheath folds. Presence of parasitic folding and associated penetrative strains seem to be controlled by differences in mechanical stratigraphy, relative thicknesses of the competent and incompetent units, and the structural relief of the underlying basement. Our present study in conjunction with other available geological, geochemical and geochronological data from the region indicates that the structures of the detachment zone are genetically related to thrust tectonics forming a part of subduction-accretion-collision tectonic history of the Neoproterozoic Gondwana suture.

Geo-Thermochronometric Insights on the Cycladic Basement and Cycladic Blueschist Unit Contact in the Southern Cyclades, Ios Island, Greece

NASA Astrophysics Data System (ADS)

Flansburg, M. E.; Stockli, D. F.; Poulaki, E. M.; Soukis, K. I.

2017-12-01

The North Cycladic Detachment System, the West Cycladic Detachment System, and the Naxos-Paros Detachment accommodated large-scale Oligo-Miocene exhumation in the backarc of the retreating Hellenic subduction zone. While bivergent detachment faults in the northern and western Cyclades are either contained within the Cycladic Blueschist Unit (CBU) or at the CBU-Upper Unit interface, the sheared contact between the CBU and the underlying Cycladic Basement in the southern Cyclades (Ios) has been debated for over 30 years, largely due to the ambiguous coexistence of both top-to-the-N and top-to-the-S shear sense indicators and a lack of robust timing information. Reliable chronostratigraphic and thermal history constraints allow us to test whether the contact is a low-angle normal fault-possibly part of a larger detachment system-or the South Cycladic Thrust by placing absolute ages on deformation, determining older over younger relationships or vice versa, and quantifying possible differential exhumation during Cenozoic extension. Zircon U-Pb dating for the granitic Basement core of Ios gave Carboniferous-Permian age and shows that surrounding Basement metasedimentary units can be divided into two groups based on detrital zircon signatures. An older group of metasedimentary rocks have maximum depositional ages (MDAs) ranging from 450 Ma to 354 Ma and predate the intrusions, and late Permian Basement paragneisses are younger than the intrusions and likely originally deposited unconformably on the older units. Samples from the CBU in northern Ios yielded MDAs ranging from Mid-Jurassic to Late Cretaceous and appear to be repeated due to either thrusting or subduction accretion and exhibit older over younger relationships. MDA data from mapped CBU at the southern end of Ios yielded Ordovician to Permian ages, calling into question their assignment as CBU, while also revealing older over younger relationships. Zircon (U-Th)/He ages for the Basement and the CBU on Ios are 9

Basement Structure and Styles of Active Tectonic Deformation in Central Interior Alaska

NASA Astrophysics Data System (ADS)

Dixit, N.; Hanks, C.

2017-12-01

Central Interior Alaska is one of the most seismically active regions in North America, exhibiting a high concentration of intraplate earthquakes approximately 700 km away from the southern Alaska subduction zone. Based on increasing seismological evidence, intraplate seismicity in the region does not appear to be uniformly distributed, but concentrated in several discrete seismic zones, including the Nenana basin and the adjacent Tanana basin. Recent seismological and neotectonics data further suggests that these seismic zones operate within a field of predominantly pure shear driven primarily by north-south crustal shortening. Although the location and magnitude of the seismic activity in both basins are well defined by a network of seismic stations in the region, the tectonic controls on intraplate earthquakes and the heterogeneous nature of Alaska's continental interior remain poorly understood. We investigated the current crustal architecture and styles of tectonic deformation of the Nenana and Tanana basins using existing geological, geophysical and geochronological datasets. The results of our study demonstrate that the basements of the basins show strong crustal heterogeneity. The Tanana basin is a relatively shallow (up to 2 km) asymmetrical foreland basin with its southern, deeper side controlled by the northern foothills of the central Alaska Range. Northeast-trending strike-slip faults within the Tanana basin are interpreted as a zone of clockwise crustal block rotation. The Nenana basin has a fundamentally different geometry; it is a deep (up to 8 km), narrow transtensional pull-apart basin that is deforming along the left-lateral Minto Fault. This study identifies two distinct modes of tectonic deformation in central Interior Alaska at present, and provides a basis for modeling the interplay between intraplate stress fields and major structural features that potentially influence the generation of intraplate earthquakes in the region.

LPT. Shield test facility (TAN645 and 646). Basement and subbasement ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LPT. Shield test facility (TAN-645 and -646). Basement and sub-basement plan. Stairway plans and details. Ralph M. Parsons 1229-17 ANP/GE-6-645-A-2. April 1957. Approved by INEEL Classification Office for public release. INEEL index code no. 037-0645/0646-00-693-107348 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

Basement control of structure in the Gettysburg rift basin, Pennsylvania and Maryland

NASA Astrophysics Data System (ADS)

Root, Samuel I.

1989-09-01

Jurassic faulting formed the 93 km long Gettysburg basin as an extensional half graben paralleling the basement structural grain. Preserved in the basin are rift-related Carnian to Rhaetian strata that were tilted 20-30° NW into a SE dipping, listric normal fault at the northwest border of the basin. Vertical displacement on the border fault approaches 10 km. The border fault developed parallel to the trend of the terminal Paleozoic Alleghenian South Mountain cleavage of the Blue Ridge basement along 80% of its extent. However, it is only roughly parallel to discordant to dip of the cleavage. Relationship of cleavage and later border faulting may be the result of persistent reactivation of the original Appalachian continental margin. Local complex structures in the half graben are related to reactivation of two subvertical, pre-Mesozoic faults that transect basement structural grain (cleavage) at a large angle. The northern Shippensburg fault was reactivated during basin normal faulting, offsetting the border fault in a right-lateral sense by 3.5 km and forming within the basin a fold and a fault sliver of basement. The southern Carbaugh-Marsh Creek fault was not reactivated, but is the locus of a 20°-30° change of trend of both the basement cleavage and later border fault. However, two large, NW trending, left-lateral wrench faults, antithetic to the Carbaugh-March Creek fault, developed here offsetting the border fault and forming en echelon folds and horst blocks of basement rock within the basin.

In vivo confocal microscopy of human cornea covered with human amniotic membrane.

PubMed

Mimura, Tatsuya; Yamagami, Satoru; Usui, Tomohiko; Honda, Norihiko; Araki, Fumiyuki; Amano, Shiro

2008-01-01

Amniotic membrane transplantation has been widely performed to reconstruct the surface of the eye and treat chemical burns or epithelial defects. However, we have difficulty observing the cornea through the opaque transplanted amniotic membrane by slit-lamp biomicroscopy. We investigated the use of confocal microscopy for observation of human corneas covered with amniotic membrane. Human amniotic membrane was placed onto the normal corneas of five volunteers aged 22-24 years. Then, all layers of the covered corneas were observed by in vivo confocal microscopy. Confocal microscopy displayed the epithelium, basement membrane, and stroma of the amniotic membrane. It also displayed the corneal epithelium. Furthermore, corneal stromal keratocytes and the corneal endothelium were clearly observed through the amniotic membrane by confocal microscopy. We demonstrated that in vivo confocal microscopy enabled us to observe all layers of corneas covered with amniotic membrane in normal human eyes. Our findings suggest that confocal microscopy may have advantages for clinical examination of the ocular surface, including all layers of the cornea.

The Fiber Grating Sensors Applied in the Deformation Measurement of Shipborne Antenna Basement

NASA Astrophysics Data System (ADS)

Liu, Yong; Chen, Jiahong; Zhao, Wenhua

2016-02-01

The optical fiber grating sensor is a novel fibre-optical passive device, its reflecting optical spectrum is linearly related with strain. It is broadly applied in the structural monitoring industry. Shipborne antenna basement is the basic supporting structure for the radar tracking movement. The bending deformation of the basement caused by ship attitude changing influences the antenna tracking precision, According to the structure of shipborne antenna basement, a distributed strain testing method based on the fibre grating sensor is approved to measure the bending deformation under the bending force. The strain-angle model is built. The regularity of the strain distribution is obtained. The finite element method is used to analyze the deformation of the antenna basement. The measuring experiment on the contractible basement mould is carried out to verify the availability of the method. The result of the experiment proves that the model is effective to apply in the deformation measurement. It provides an optimized method for the distribution of the fiber grating sensor in the actual measuring process.

43. Dressing rooms and corridor in basement on west side ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

43. Dressing rooms and corridor in basement on west side of building, looking south. (July 1991) A nearly identical corridor with dressing rooms ran along the east side of the basement, and the two corridors were connected by a short hallway at the north end. The theatre seats seen in hallway in this photo are from Seattle's Orpheum Theatre which was demolished in 1967. - Fox Theater, Seventh Avenue & Olive Way, Seattle, King County, WA

The role of basement inheritance faults in the recent fracture system of the inner shelf around Alboran Island, Western Mediterranean

NASA Astrophysics Data System (ADS)

Maestro-González, A.; Bárcenas, P.; Vázquez, J. T.; Díaz-Del-Río, V.

2008-02-01

Fractures associated with volcanic rock outcrops on the inner shelf of Alboran Island, Western Mediterranean, were mapped on the basis of a side-scan sonar mosaic. Absolute maximum fracture orientation frequency is NW SE to NNW SSE, with several sub-maxima oriented NNE SSW, NE SW and ENE WSW. The origin of the main fracture systems in Neogene and Quaternary rocks of the Alboran Basin (south Spain) appears to be controlled by older structures, namely NE SW and WNW ESE to NW SE faults which cross-cut the basement. These faults, pre-Tortonian in origin, have been reactivated since the early Neogene in the form of strike-slip and extensional movements linked to the recent stress field in this area. Fracture analysis of volcanic outcrops on the inner continental shelf of Alboran Island suggests that the shelf has been deformed into a narrow shear zone limited by two NE SW-trending, sub-parallel high-angle faults, the main orientation and density of which have been influenced by previous WNW ESE to NW SE basement fractures.

3D depth-to-basement and density contrast estimates using gravity and borehole data

NASA Astrophysics Data System (ADS)

Barbosa, V. C.; Martins, C. M.; Silva, J. B.

2009-05-01

We present a gravity inversion method for simultaneously estimating the 3D basement relief of a sedimentary basin and the parameters defining the parabolic decay of the density contrast with depth in a sedimentary pack assuming the prior knowledge about the basement depth at a few points. The sedimentary pack is approximated by a grid of 3D vertical prisms juxtaposed in both horizontal directions, x and y, of a right-handed coordinate system. The prisms' thicknesses represent the depths to the basement and are the parameters to be estimated from the gravity data. To produce stable depth-to-basement estimates we impose smoothness on the basement depths through minimization of the spatial derivatives of the parameters in the x and y directions. To estimate the parameters defining the parabolic decay of the density contrast with depth we mapped a functional containing prior information about the basement depths at a few points. We apply our method to synthetic data from a simulated complex 3D basement relief with two sedimentary sections having distinct parabolic laws describing the density contrast variation with depth. Our method retrieves the true parameters of the parabolic law of density contrast decay with depth and produces good estimates of the basement relief if the number and the distribution of boreholes are sufficient. We also applied our method to real gravity data from the onshore and part of the shallow offshore Almada Basin, on Brazil's northeastern coast. The estimated 3D Almada's basement shows geologic structures that cannot be easily inferred just from the inspection of the gravity anomaly. The estimated Almada relief presents steep borders evidencing the presence of gravity faults. Also, we note the existence of three terraces separating two local subbasins. These geologic features are consistent with Almada's geodynamic origin (the Mesozoic breakup of Gondwana and the opening of the South Atlantic Ocean) and they are important in understanding

Petrophysics Features of the Hydrocarbon Reservoirs in the Precambrian Crystalline Basement

NASA Astrophysics Data System (ADS)

Plotnikova, Irina

2014-05-01

A prerequisite for determining the distribution patterns of reservoir zones on the section of crystalline basement (CB) is the solution of a number of problems connected with the study of the nature and structure of empty spaces of reservoirs with crystalline basement (CB) and the impact of petrological, and tectonic factors and the intensity of the secondary transformation of rocks. We decided to choose the Novoelhovskaya well # 20009 as an object of our research because of the following factors. Firstly, the depth of the drilling of the Precambrian crystalline rocks was 4077 m ( advance heading - 5881 m) and it is a maximum for the Volga-Urals region. Secondly, petrographic cut of the well is made on core and waste water, and the latter was sampled regularly and studied macroscopically. Thirdly, a wide range of geophysical studies were performed for this well, which allowed to identify promising areas of collector with high probability. Fourth, along with geological and technical studies that were carried out continuously (including washing and bore hole redressing periods), the studies of the gaseous component of deep samples of clay wash were also carried out, which indirectly helped us estimate reservoir properties and fluid saturation permeable zones. As a result of comprehensive analysis of the stone material and the results of the geophysical studies we could confidently distinguish 5 with strata different composition and structure in the cut of the well. The dominating role in each of them is performed by rocks belonging to one of the structural-material complexes of Archean, and local variations in composition and properties are caused by later processes of granitization on different stages and high temperature diaphthoresis imposed on them. Total capacity of reservoir zones identified according to geophysical studies reached 1034.2 m, which corresponds to 25.8% of the total capacity of 5 rock masses. However, the distribution of reservoirs within the cut

Origin of a major cross-element zone: Moroccan Rif

NASA Astrophysics Data System (ADS)

Morley, C. K.

1987-08-01

Alpine age (Oligocene-Miocene) deformation in the western Mediterranean formed the Rif mountain belt of northern Morocco. A linear east-northeast-west-southwest trend of cross elements from Jebah (Mediterranean coast) to Arbaoua (near the Atlantic coast) extends through several thrust sheets in the western Rif. The cross elements are manifest as a lateral ramp, the northern limit of a large culmination, and they affect syntectonic turbidite sandstone distribution. Gravity anomalies indicate that the cross-element zone is coincident with a transition zone from normal thickness to thinner continental crust. It is suggested that an early Mesozoic strike-slip fault system related to rifting of North America from North Africa caused a strong east-northeast-west-southwest, basement block-fault trend to form on the normal thickness side of the thick-to-thin continental crustal transition zone. This trend later influenced the position of the Alpine age cross-element zone that traverses several different Mesozoic and Tertiary basins, inverted during the Alpine deformation.

Precambrrian crustal evolution in the great falls tectonic zone

NASA Astrophysics Data System (ADS)

Gifford, Jennifer N.

The Great Falls Tectonic Zone (GFTZ) is a zone of northeast trending geological structures in central Montana that parallel structures in the underlying basement. U-Pb zircon and Nd isotopic data from the Little Belt Mountains (LBM) suggest that the GFTZ formed at ~1.86 to 1.80 Ga due to ocean subduction followed by collision between the Archean Wyoming Province (WP) and Medicine Hat Block (MHB). This study characterizes the GFTZ basement by geochronological and geochemical analysis of crustal xenoliths collected from Montana Alkali Province volcanics and exposed basement rock in the Little Rocky Mountains (LRM). Xenoliths collected from the Grassrange and Missouri Breaks diatremes and volcanics in the Bearpaw and Highwood Mountains have igneous crystallization ages from ~1.7 Ga to 1.9 Ga and 2.4 Ga to 2.7 Ga, and metamorphic ages from ~1.65 Ga to 1.84 Ga. Zircon Lu-Hf and whole-rock Sm-Nd data indicate that the xenoliths originated from reworked older continental crust mixed with mantle-derived components in all cases. Trace element patterns show fluid mobile element enrichments and fluid immobile element depletions suggestive of a subduction origin. Igneous ages in the LRM range older, from ~2.4 Ga to 3.2 Ga. Geochemical evidence suggests that the LRM meta-igneous units also formed in a subduction setting. Detrital zircon ages span the early Paleoproterozoic to Mesoarchean, with abundant 2.8 Ga ages. Zircon U-Pb igneous crystallization age data from xenoliths and the LRM are consistent with U-Pb zircon igneous crystallization ages from the MHB, suggesting that this segment of the GFTZ shares an affinity with concealed MHB crust. Published detrital zircon ages from the northern Wyoming Province reveal more abundant >3.0 Ga ages than the MHB or GFTZ samples. These geochronologic and geochemical data from the xenoliths and LRM samples allow for a refined model for crustal evolution in the GFTZ. Subduction under the Neoarchean to Paleoproterozoic crust of the MHB

Relationship of Basal laminar deposit and membranous debris to the clinical presentation of early age-related macular degeneration.

PubMed

Sarks, Shirley; Cherepanoff, Svetlana; Killingsworth, Murray; Sarks, John

2007-03-01

To correlate basal laminar deposit (BLamD) and membranous debris, including basal linear deposit (BLinD), with the evolution of early age-related macular degeneration (AMD). A clinicopathologic collection of 132 eyes with a continuous layer of BLamD was reviewed. The thickness and type of BLamD and the sites of membranous debris deposition were correlated with the clinical progression of the disease. Two types of BLamD, termed early and late, were identified based on light microscopic appearance by using the picro-Mallory stain. The progressive accumulation of late type BLamD correlated well with increasing BLamD thickness, advancing RPE degeneration, poorer vision, increasing age, and clinically evident pigment changes. Membranous debris initially accumulated diffusely as BLinD, most eyes with BLinD and early BLamD remaining funduscopically normal. However, membranous debris also formed focal collections as basal mounds internal to the RPE basement membrane and as soft drusen external to the basement membrane. Eyes in which membranous debris remained confined to basal mounds belonged to older patients with poorer vision, whereas patients with soft drusen were younger and had better vision. The presence of BLinD and early BLamD define threshold AMD, which manifests clinically as a normal fundus. Although late BLamD correlates most closely with clinical pigment abnormalities, it is the quantity and sites of membranous debris accumulation that appear to determine whether the disease develops pigment changes only or follows the alternative pathway of soft drusen formation with its attendant greater risk of choroidal neovascularization (CNV).

The basement of the Mount Athos peninsula, northern Greece: insights from geochemistry and zircon ages

NASA Astrophysics Data System (ADS)

Himmerkus, F.; Zachariadis, P.; Reischmann, T.; Kostopoulos, D.

2012-09-01

The Mount Athos Peninsula is situated in the south-easternmost part of the Chalkidiki Peninsula in northern Greece. It belongs to the Serbo-Macedonian Massif (SMM), a large basement massif within the Internal Hellenides. The south-eastern part of the Mount Athos peninsula is built by fine-grained banded biotite gneisses and migmatites forming a domal structure. The southern tip of the peninsula, which also comprises Mount Athos itself, is built by limestone, marble and low-grade metamorphic rocks of the Chortiatis Unit. The northern part and the majority of the western shore of the Mount Athos peninsula are composed of highly deformed rocks belonging to a tectonic mélange termed the Athos-Volvi-Suture Zone (AVZ), which separates two major basement units: the Vertiskos Terrane in the west and the Kerdillion Unit in the east. The rock-types in this mélange range from metasediments, marbles and gneisses to amphibolites, eclogites and peridotites. The gneisses are tectonic slivers of the adjacent basement complexes. The mélange zone and the gneisses were intruded by granites (Ierissos, Ouranoupolis and Gregoriou). The Ouranoupolis intrusion obscures the contact between the mélange and the gneisses. The granites are only slightly deformed and therefore postdate the accretionary event that assembled the units and created the mélange. Pb-Pb- and U-Pb-SHRIMP-dating of igneous zircons of the gneisses and granites of the eastern Athos peninsula in conjunction with geochemical and isotopic analyses are used to put Athos into the context of a regional tectonic model. The ages form three clusters: The basement age is indicated by two samples that yielded Permo-Carboniferous U-Pb-ages of 292.6 ± 2.9 Ma and 299.4 ± 3.5 Ma. The main magmatic event of the granitoids now forming the gneiss dome is dated by Pb-Pb-ages between 140.0 ± 2.6 Ma and 155.7 ± 5.1 Ma with a mean of 144.7 ± 2.4 Ma. A within-error identical age of 146.6 ± 2.3 Ma was obtained by the U

Extracellular Matrix Signaling from the Cellular Membrane Skeleton to the Nuclear Skeleton: A Model of Gene Regulation

PubMed Central

Lelièvre, Sophie; Weaver, Valerie M.; Bissell, Mina J.

2010-01-01

It is well established that cells must interact with their microenvironment and that such interaction is crucial for coordinated function and homeostasis. However, how cells receive and integrate external signals leading to gene regulation is far from understood. It is now appreciated that two classes of cooperative signals are implicated: a soluble class including hormones and growth factors and a class of insoluble signals emanating from the extracellular matrix (ECM) directly through contact with the cell surface. Using 3-dimensional culture systems and transgenic mice, we have been able to identify some of the elements of this ECM-signaling pathway responsible for gene regulation in rodent mammary gland differentiation and involution. Our major observations are 1) the requirement for a laminin-rich basement membrane; 2) the existence of a cooperative signaling pathway between basement membrane and the lactogenic hormone prolactin (PRL); 3) the importance of β1-integrins and bHLH transcription factor(s) and the presence of DNA response elements (exemplified by BCE-1, located on a milk protein gene, β-casein); and 4) the induction of mammary epithelial cell programmed cell death following degradation of basement membrane. We hypothesize that this cooperative signaling between ECM and PRL may be achieved through integrin- and laminin-directed restructuring of the cytoskeleton leading to profound changes in nuclear architecture and transcription factor localization. We postulate that the latter changes allow the prolactin signal to activate transcription of the β-casein gene. To further understand the molecular mechanisms underlying ECM and hormonal cooperative signaling, we are currently investigating ECM regulation of a “solid-state” signaling pathway including ECM fiber proteins, plasma membrane receptors, cytoskeleton, nuclear matrix and chromatin. We further postulate that disruption of such a pathway may be implicated in cell disorders including

Gradient zone boundary control in salt gradient solar ponds

DOEpatents

Hull, John R.

1984-01-01

A method and apparatus for suppressing zone boundary migration in a salt gradient solar pond includes extending perforated membranes across the pond at the boundaries, between the convective and non-convective zones, the perforations being small enough in size to prevent individual turbulence disturbances from penetrating the hole, but being large enough to allow easy molecular diffusion of salt thereby preventing the formation of convective zones in the gradient layer. The total area of the perforations is a sizable fraction of the membrane area to allow sufficient salt diffusion while preventing turbulent entrainment into the gradient zone.

Gradient zone-boundary control in salt-gradient solar ponds

DOEpatents

Hull, J.R.

1982-09-29

A method and apparatus for suppressing zone boundary migration in a salt gradient solar pond includes extending perforated membranes across the pond at the boundaries, between the convective and non-convective zones, the perforations being small enough in size to prevent individual turbulence disturbances from penetrating the hole, but being large enough to allow easy molecular diffusion of salt thereby preventing the formation of convective zones in the gradient layer. The total area of the perforations is a sizeable fraction of the membrane area to allow sufficient salt diffusion while preventing turbulent entrainment into the gradient zone.

Precambrian Basement Structure Map of the Continental United States - An Interpretation of Geologic and Aeromagnetic Data

USGS Publications Warehouse

Sims, Paul K.; Saltus, Richard W.; Anderson, Eric D.

2008-01-01

The Precambrian basement rocks of the continental United States are largely covered by younger sedimentary and volcanic rocks, and the availability of updated aeromagnetic data (NAMAG, 2002) provides a means to infer major regional basement structures and tie together the scattered, but locally abundant, geologic information. Precambrian basement structures in the continental United States have strongly influenced later Proterozoic and Phanerozoic tectonism within the continent, and there is a growing awareness of the utility of these structures in deciphering major younger tectonic and related episodes. Interest in the role of basement structures in the evolution of continents has been recently stimulated, particularly by publications of the Geological Society of London (Holdsworth and others, 1998; Holdsworth and others, 2001). These publications, as well as others, stress the importance of reactivation of basement structures in guiding the subsequent evolution of continents. Knowledge of basement structures is an important key to understanding the geology of continental interiors.

Autoimmune Subepidermal Bullous Diseases of the Skin and Mucosae: Clinical Features, Diagnosis, and Management.

PubMed

Amber, Kyle T; Murrell, Dedee F; Schmidt, Enno; Joly, Pascal; Borradori, Luca

2018-02-01

Autoimmune subepidermal blistering diseases of the skin and mucosae constitute a large group of sometimes devastating diseases, encompassing bullous pemphigoid, gestational pemphigoid, mucous membrane pemphigoid, epidermolysis bullosa acquisita, and anti-p200 pemphigoid. Their clinical presentation is polymorphic. These autoimmune blistering diseases are associated with autoantibodies that target distinct components of the basement membrane zone of stratified epithelia. These autoantigens represent structural proteins important for maintenance of dermo-epidermal integrity. Bullous pemphigoid (BP) is the most common subepidermal autoimmune blistering disease of the skin and mucosae. Although the disease typically presents with a generalized blistering eruption associated with itch, atypical variants with either localized bullous lesions or "non-bullous" presentations are observed in approximately 20% of patients. A peculiar form of BP typically associated with pregnancy is pemphigoid gestationis. In anti-p200 pemphigoid, patients present with tense blisters on erythematosus or normal skin resembling BP, with a predilection for acral surfaces. These patients have antibodies targeting the 200-kDa basement membrane protein. Epidermolysis bullosa is a rare autoimmune blistering disease associated with autoantibodies against type VII collagen that can have several phenotypes including a classical form mimicking dystrophic epidermolysis bullosa, an inflammatory presentation mimicking BP, or mucous membrane pemphigoid-like lesions. Mucous membrane pemphigoid (MMP) is the term agreed upon by international consensus for an autoimmune blistering disorder, which affects one or more mucous membrane and may involve the skin. The condition involves a number of different autoantigens in the basement membrane zone. It may result in severe complications from scarring, such as blindness and strictures. Diagnosis of these diseases relies on direct immunofluorescence microscopy studies

Variable deep structure of a midcontinent fault and fold zone from seismic reflection: La Salle deformation belt, Illinois basin

USGS Publications Warehouse

McBride, J.H.

1997-01-01

Deformation within the United States mid-continent is frequently expressed as quasilinear zones of faulting and folding, such as the La Salle deformation belt, a northwest-trending series of folds cutting through the center of the Illinois basin. Seismic reflection profiles over the southern La Salle deformation belt reveal the three-dimensional structural style of deformation in the lower Paleozoic section and uppermost Precambrian(?) basement. Individual profiles and structural contour maps show for the first time that the folds of the La Salle deformation belt are underlain at depth by reverse faults that disrupt and offset intrabasement structure, offset the top of interpreted Precambrian basement, and accommodate folding of overlying Paleozoic strata. The folds do not represent development of initial dips by strata deposited over a preexisting basement high. Rather, the structures resemble subdued "Laramide-style" forced folds, in that Paleozoic stratal reflectors appear to be flexed over a fault-bounded basement uplift with the basement-cover contact folded concordantly with overlying strata. For about 40 km along strike, the dominant faults reverse their dip direction, alternating between east and west. Less well expressed antithetic or back thrusts appear to be associated with the dominant faults and could together describe a positive flower structure. The overall trend of this part of the La Salle deformation belt is disrupted by along-strike discontinuities that separate distinct fold culminations. Observations of dual vergence and along-strike discontinuities suggest an original deformation regime possibly involving limited transpression associated with distant late Paleozoic Appalachian-Ouachita mountain building. Moderate-magnitude earthquakes located west of the western flank of the La Salle deformation belt have reverse and strike-slip mechanisms at upper trustai depths, which might be reactivating deep basement faults such as observed in this study

Nonautonomous Roles of MAB-5/Hox and the Secreted Basement Membrane Molecule SPON-1/F-Spondin in Caenorhabditis elegans Neuronal Migration.

PubMed

Josephson, Matthew P; Miltner, Adam M; Lundquist, Erik A

2016-08-01

Nervous system development and circuit formation requires neurons to migrate from their birthplaces to specific destinations.Migrating neurons detect extracellular cues that provide guidance information. In Caenorhabditis elegans, the Q right (QR) and Q left (QL) neuroblast descendants migrate long distances in opposite directions. The Hox gene lin-39 cell autonomously promotes anterior QR descendant migration, and mab-5/Hox cell autonomously promotes posterior QL descendant migration. Here we describe a nonautonomous role of mab-5 in regulating both QR and QL descendant migrations, a role masked by redundancy with lin-39 A third Hox gene, egl-5/Abdominal-B, also likely nonautonomously regulates Q descendant migrations. In the lin-39 mab-5 egl-5 triple mutant, little if any QR and QL descendant migration occurs. In addition to well-described roles of lin-39 and mab-5 in the Q descendants, our results suggest that lin-39, mab-5, and egl-5 might also pattern the posterior region of the animal for Q descendant migration. Previous studies showed that the spon-1 gene might be a target of MAB-5 in Q descendant migration. spon-1 encodes a secreted basement membrane molecule similar to vertebrate F-spondin. Here we show that spon-1 acts nonautonomously to control Q descendant migration, and might function as a permissive rather than instructive signal for cell migration. We find that increased levels of MAB-5 in body wall muscle (BWM) can drive the spon-1 promoter adjacent to the Q cells, and loss of spon-1 suppresses mab-5 gain of function. Thus, MAB-5 might nonautonomously control Q descendant migrations by patterning the posterior region of the animal to which Q cells respond. spon-1 expression from BWMs might be part of the posterior patterning necessary for directed Q descendant migration. Copyright © 2016 by the Genetics Society of America.

Nonautonomous Roles of MAB-5/Hox and the Secreted Basement Membrane Molecule SPON-1/F-Spondin in Caenorhabditis elegans Neuronal Migration

PubMed Central

Josephson, Matthew P.; Miltner, Adam M.; Lundquist, Erik A.

2016-01-01

Nervous system development and circuit formation requires neurons to migrate from their birthplaces to specific destinations.Migrating neurons detect extracellular cues that provide guidance information. In Caenorhabditis elegans, the Q right (QR) and Q left (QL) neuroblast descendants migrate long distances in opposite directions. The Hox gene lin-39 cell autonomously promotes anterior QR descendant migration, and mab-5/Hox cell autonomously promotes posterior QL descendant migration. Here we describe a nonautonomous role of mab-5 in regulating both QR and QL descendant migrations, a role masked by redundancy with lin-39. A third Hox gene, egl-5/Abdominal-B, also likely nonautonomously regulates Q descendant migrations. In the lin-39mab-5egl-5 triple mutant, little if any QR and QL descendant migration occurs. In addition to well-described roles of lin-39 and mab-5 in the Q descendants, our results suggest that lin-39, mab-5, and egl-5 might also pattern the posterior region of the animal for Q descendant migration. Previous studies showed that the spon-1 gene might be a target of MAB-5 in Q descendant migration. spon-1 encodes a secreted basement membrane molecule similar to vertebrate F-spondin. Here we show that spon-1 acts nonautonomously to control Q descendant migration, and might function as a permissive rather than instructive signal for cell migration. We find that increased levels of MAB-5 in body wall muscle (BWM) can drive the spon-1 promoter adjacent to the Q cells, and loss of spon-1 suppresses mab-5 gain of function. Thus, MAB-5 might nonautonomously control Q descendant migrations by patterning the posterior region of the animal to which Q cells respond. spon-1 expression from BWMs might be part of the posterior patterning necessary for directed Q descendant migration. PMID:27225683

Structural-geophysical model of the basement complex of the Aden-Red Sea region

NASA Astrophysics Data System (ADS)

Isaev, E. N.

1987-11-01

A relief map of the basement complex underlying the volcanogenic sedimentary cover has been constructed on the basis of composite Bouguer anomaly maps and maps of magnetic anomalies ΔT. Seismic and geological data on the Gulf of Aden and the Red Sea as well as on adjacent areas of Africa and Arabia have also been used. The mid-Red Sea and mid-Aden uplifts (similar to the mid-oceanic ones) as well as the foredeep have been identified. The thickness of cover in the foredeeps is 6-8 km. A regional negative Bouguer anomaly crosses the Aden-Red Sea rift system and includes the area of young volcanism. Intensive linear magnetic anomalies are traceable only within the area of overlap of the rift system and the zone of young volcanism. Rift system apophyses have advanced into the continent and their nature is similar to that of the Afar triangle.

33. VIEW OF BASEMENT UNDER EAST BOILER ROOM LOOKING TOWARD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. VIEW OF BASEMENT UNDER EAST BOILER ROOM LOOKING TOWARD WEST BOILER ROOM BASEMENT THROUGH THE ASH TRANSFER TUNNEL. ASH HOPPER FOR BOILER 900 IS ON THE RIGHT. NOTE THE TRACKS ALONG THE FLOOR OF THE TUNNEL. A SMALL ELECTRIC LOCOMOTIVE HAULED CARS FOR TRANSFERRING ASH FROM BOILERS TO DISPOSAL SITES OUTSIDE THE BUILDING. THIS SYSTEM BECAME OBSOLETE IN 1938 WHEN BOILERS IN THE WEST BOILER ROOM WERE REMOVED AND PULVERIZED COAL WAS ADOPTED AS THE FUEL. - New York, New Haven & Hartford Railroad, Cos Cob Power Plant, Sound Shore Drive, Greenwich, Fairfield County, CT

Ultrathin Transparent Membranes for Cellular Barrier and Co-Culture Models

PubMed Central

Carter, Robert N.; Casillo, Stephanie M.; Mazzocchi, Andrea R.; DesOrmeaux, Jon-Paul S.; Roussie, James A.; Gaborski, Thomas R.

2017-01-01

Typical in vitro barrier and co-culture models rely upon thick semi-permeable polymeric membranes that physically separate two compartments. Polymeric track-etched membranes, while permeable to small molecules, are far from physiological with respect to physical interactions with co-cultured cells and are not compatible with high-resolution imaging due to light scattering and autofluorescence. Here we report on an optically transparent ultrathin membrane with porosity exceeding 20%. We optimize deposition and annealing conditions to create a tensile and robust porous silicon dioxide membrane that is comparable in thickness to the vascular basement membrane (100–300 nm). We demonstrate that human umbilical vein endothelial cells (HUVECs) spread and proliferate on these membranes similarly to control substrates. Additionally, HUVECs are able to transfer cytoplasmic cargo to adipose-derived stem cells when they are co-cultured on opposite sides of the membrane, demonstrating its thickness supports physiologically relevant cellular interactions. Lastly, we confirm that these porous glass membranes are compatible with lift-off processes yielding membrane sheets with an active area of many square centimeters. We believe that these membranes will enable new in vitro barrier and co-culture models while offering dramatically improved visualization compared to conventional alternatives. PMID:28140345

Changes in carbohydrate content and membrane stability of two ecotypes of Calamagrostis arundinacea growing at different elevations in the drawdown zone of the Three Gorges Reservoir.

PubMed

Lei, Shutong; Zeng, Bo; Yuan, Zhi; Su, Xiaolei

2014-01-01

The Three Gorges project has caused many ecosystem problems. Ecological restoration using readily-available plants is an effective way of mitigating environmental impacts. Two perennial submergence-tolerant ecotypes of Calamagrostis arundinacea were planted in an experimental field in the drawdown zone. Responses of the two plant ecotypes to flooding stress in the drawdown zone were unknown. Carbohydrate content and membrane stability, two key factors for survival of plants under flooding stress, of two ecotypes (designated "dwarf" and "green") of C. arundinacea growing at different elevations of the drawdown zone were investigated. Live stems (LS) and dead stems (DS) of the two plant ecotypes at eight elevations (175, 170, 162, 160, 158, 155, 152 m and 149 m) were sampled. Contents of soluble sugar, starch and malondialdehyde (MDA), as well as plasma membrane permeability of live stems were measured. The lowest elevations for survival of dwarf and green C. arundinacea were 160 m and 158 m, respectively. Soluble sugar content of live stems of both ecotypes decreased with elevation, with amounts from an elevation of 170 m being lower than from an elevation of 175 m. MDA content and plasma membrane permeability in live stems of green C. arundinacea did not increase with the decrease in elevation, while these measures in dwarf C. arundinacea from an elevation of 162 m were significantly higher than from an elevation of 175 m. Carbohydrate content, especially soluble sugar content, in both ecotypes was more sensitive to flooding stress than membrane stability. Green C. arundinacea had a higher tolerance to submergence than dwarf C. arundinacea, and thus green C. arundinacea can be planted at lower elevations than dwarf C. arundinacea.

CHARACTER AND REGIONAL SIGNIFICANCE OF GREAT FALLS TECTONIC ZONE, EAST-CENTRAL IDAHO AND WEST-CENTRAL MONTANA.

USGS Publications Warehouse

O'Neill, J. Michael; Lopez, David A.

1985-01-01

The Great Falls tectonic zone, here named, is a belt of diverse northeast-trending geologic features that can be traced from the Idaho batholith in the Cordilleran miogeocline, across thrust-belt structures and basement rocks of west-central and southwestern Montana, through cratonic rocks of central Montana, and into southwestern-most Saskatchewan, Canada. Geologic mapping in east-central Idaho and west-central Montana has outlined a continuous zone of high-angle faults and shear zones. Recurrent fault movement in this zone and strong structural control over igneous intrusion suggest a fundamental tectonic feature that has influenced the tectonic development of the Idaho-Montana area from a least middle Proterozoic time to the present. Refs.

Structural analysis of the Gachsar sub-zone in central Alborz range; constrain for inversion tectonics followed by the range transverse faulting

NASA Astrophysics Data System (ADS)

Yassaghi, A.; Naeimi, A.

2011-08-01

Analysis of the Gachsar structural sub-zone has been carried out to constrain structural evolution of the central Alborz range situated in the central Alpine Himalayan orogenic system. The sub-zone bounded by the northward-dipping Kandovan Fault to the north and the southward-dipping Taleghan Fault to the south is transversely cut by several sinistral faults. The Kandovan Fault that controls development of the Eocene rocks in its footwall from the Paleozoic-Mesozoic units in the fault hanging wall is interpreted as an inverted basin-bounding fault. Structural evidences include the presence of a thin-skinned imbricate thrust system propagated from a detachment zone that acts as a footwall shortcut thrust, development of large synclines in the fault footwall as well as back thrusts and pop-up structures on the fault hanging wall. Kinematics of the inverted Kandovan Fault and its accompanying structures constrain the N-S shortening direction proposed for the Alborz range until Late Miocene. The transverse sinistral faults that are in acute angle of 15° to a major magnetic lineament, which represents a basement fault, are interpreted to develop as synthetic Riedel shears on the cover sequences during reactivation of the basement fault. This overprinting of the transverse faults on the earlier inverted extensional fault occurs since the Late Miocene when the south Caspian basin block attained a SSW movement relative to the central Iran. Therefore, recent deformation in the range is a result of the basement transverse-fault reactivation.

Structural and Geophysical Characterization of Oklahoma Basement

NASA Astrophysics Data System (ADS)

Morgan, C.; Johnston, C. S.; Carpenter, B. M.; Reches, Z.

2017-12-01

Oklahoma has experienced a large increase in seismicity since 2009 that has been attributed to wastewater injection. Most earthquakes, including four M5+ earthquakes, nucleated at depths > 4 km, well within the pre-Cambrian crystalline basement, even though wastewater injection occurred almost exclusively in the sedimentary sequence above. To better understand the structural characteristics of the rhyolite and granite that makeup the midcontinent basement, we analyzed a 150 m long core recovered from a basement borehole (Shads 4) in Rogers County, NE Oklahoma. The analysis of the fracture network in the rhyolite core included measurements of fracture inclination, aperture, and density, the examination fracture surface features and fill minerology, as well as x-ray diffraction analysis of secondary mineralization. We also analyzed the highly fractured and faulted segments of the core with a portable gamma-ray detector, magnetometer, and rebound hammer. The preliminary analysis of the fractures within the rhyolite core showed: (1) Fracture density increasing with depth by a factor of 10, from 4 fractures/10m in the upper core segment to 40 fracture/10m at 150 m deeper. (2) The fractures are primarily sub-vertical, inclined 10-20° from the axis of the vertical core. (3) The secondary mineralization is dominated by calcite and epidote. (4) Fracture aperture ranges from 0.35 to 2.35mm based on the thickness of secondary filling. (5) About 8% of the examined fractures display slickenside striations. (6) Increases of elasticity (by rebound hammer) and gamma-ray emissions are systematically correlated with a decrease in magnetic susceptibility in core segments of high fracture density and/or faulting; this observation suggests diagenetic fracture re-mineralization.

Structural-tectonic zoning of the Arctic

NASA Astrophysics Data System (ADS)

Petrov, Oleg; Sobolev, Nikolay; Morozov, Andrey; Shokalsky, Sergey; Kashubin, Sergey; Grikurov, Garrik; Tolmacheva, Tatiana; Rekant, Pavel; Petrov, Evgeny

2017-04-01

Structural-tectonic zoning of the Arctic is based on the processing of geological and geophysical data and bottom sampling materials produced within the project "Atlas of Geological Maps of the Circumpolar Arctic." Zoning of the Arctic territories has been conducted taking into account the Earth's crust types, age of consolidated basement, and features of geological structure of the sedimentary cover. Developed legend for the zoning scheme incorporates five main groups of elements: continental and oceanic crust, folded platform covers, accretion-collision systems, and provinces of continental cover basalts. An important feature of the structural-tectonic zoning scheme is designation of continental crust in the central regions of the Arctic Ocean, the existence of which is assumed on the basis of numerous geological data. It has been found that most of the Arctic region has continental crust with the exception of the Eurasian Basin and the central part of the Canada Basin, which are characterized by oceanic crust type. Thickness of continental crust from seismic data varies widely: from 30-32 km on the Mendeleev Rise to 18-20 km on the Lomonosov Ridge, decreasing to 8-10 km in rift structures of the Podvodnikov-Makarov Basin at the expense of reduction of the upper granite layer. New data confirm similar basement structure on the western and eastern continental margins of the Eurasian oceanic basin. South to north, areas of Neoproterozoic (Baikalian) and Paleozoic (Ellesmerian) folding are successively distinguished. Neoproterozoic foldbelt is observed in Central Taimyr (Byrranga Mountains). Continuation of this belt in the eastern part of the Arctic is Novosibirsk-Chukchi fold system. Ellesmerian orogen incorporates the northernmost areas of Taimyr and Severnaya Zemlya, wherefrom it can be traced to the Geofizikov Spur of the Lomonosov Ridge and further across the De Long Archipelago and North Chukchi Basin to the north of Alaska Peninsula and in the Beaufort Sea

Changes in the basal membrane of dorsal root ganglia Schwann cells explain the biphasic pattern of the peripheral neuropathy in streptozotocin-induced diabetic rats.

PubMed

Becker, Maria; Benromano, Tali; Shahar, Abraham; Nevo, Zvi; Pick, Chaim G

2014-12-01

Peripheral neuropathy is one of the main complications of diabetes mellitus. The current study demonstrated the bimodal pattern of diabetic peripheral neuropathy found in the behavioral study of pain perception in parallel to the histopathological findings in dorsal root ganglia (DRGs) neurons and satellite Schwann cell basement membranes. A gradual decrease in heparan sulfate content, with a reciprocal increase in deposited laminin in the basement membranes of dorsal root ganglia Schwann cells, was shown in streptozotocin-treated rats. In addition, the characteristic biphasic pain profiles were demonstrated in diabetic rats, as shown by hypersensitivity at the third week and hyposensitivity at the tenth week post-streptozotocin injection, accompanied by a continuous decrease in the sciatic nerve conduction velocity. It appears that these basal membrane abnormalities in content of heparan sulfate and laminin, noticed in diabetic rats, may underline the primary damage in dorsal ganglion sensory neurons, simultaneously with the bimodal painful profile in diabetic peripheral neuropathy, simulating the scenario of filtration rate in diabetic kidney.

Fault distribution in the Precambrian basement of South Norway

NASA Astrophysics Data System (ADS)

Gabrielsen, Roy H.; Nystuen, Johan Petter; Olesen, Odleiv

2018-03-01

Mapping of the structural pattern by remote sensing methods (Landsat, SPOT, aerial photography, potential field data) and field study of selected structural elements shows that the cratonic basement of South Norway is strongly affected by a regular lineament pattern that encompasses fault swarms of different orientation, age, style, attitude and frequency. Albeit counting numerous fault and fracture populations, the faults are not evenly distributed and N-S to NNE-SSW/NNW-SSE and NE-SE/ENE-WSW-systems are spatially dominant. N-S to NNW-SSE structures can be traced underneath the Caledonian nappes to the Western Gneiss Region in western and central South Norway, emphasizing their ancient roots. Dyke swarms of different ages are found within most of these zones. Also, the Østfold, Oslo-Trondheim and the Mandal-Molde lineament zones coincide with trends of Sveconorwegian post-collision granites. We conclude that the N-S-trend includes the most ancient structural elements, and that the trend can be traced back to the Proterozoic (Svecofennian and Sveconorwegian) orogenic events. Some of the faults may have been active in Neoproterozoic times as marginal faults of rift basins at the western margin of Baltica. Remnants of such fault activity have survived in the cores of many of the faults belonging to this system. The ancient systems of lineaments were passively overridden by the Caledonian fold-and-thrust system and remained mostly, but note entirely inactive throughout the Sub-Cambrian peneplanation and the Caledonian orogenic collapse in the Silurian-Devonian. The system was reactivated in extension from Carboniferous times, particularly in the Permian with the formation of the Oslo Rift and parts of it remain active to the Present, albeit by decreasing extension and fault activity.

Differential expression of basement membrane type IV collagen α2 and α6 chains as a prognostic factor in patients with extrahepatic bile duct carcinoma.

PubMed

Hirashima, Kotaro; Iyama, Ken-Ichi; Baba, Yoshifumi; Honda, Yumi; Sado, Yoshikazu; Ninomiya, Yoshifumi; Watanabe, Masayuki; Takamori, Hiroshi; Beppu, Toru; Baba, Hideo

2013-03-01

The destruction of the basement membrane (BM) is the first step in cancer invasion and metastasis. Type IV collagen is a major component of the BM, and is composed of six genetically distinct α(IV) chains; α1(IV) to α6(IV). The loss of α5(IV) and α6(IV) chains from the epithelial BM at the early stage of cancer invasion has been reported in several types of cancers. However, the expression of α5(IV) and α6(IV) chains in extrahepatic bile duct carcinoma (EBDC) remains unclear. We examined the expression of α(IV) chains by immunohistochemistry using 71 resected EBDC specimens. Prognostic significance of α(IV) chains was examined by Cox regression and Kaplan-Meier analyses. In the invasive cancer, the expression of α6(IV) chain in the BM was lost partially or completely preceded by the loss of α2(IV) chain. The loss of α6(IV) chain in the BM of the invasive cancer was related to the tumor classification, TNM stages, and the expression of α2(IV) chain. The patients with α2(IV)-negative and α6(IV)-negative chains had significantly poorer prognosis than those with α2(IV)-positive and α6(IV)-positive/negative chains (P = 0.04). The loss of α2(IV) and α6(IV) chains might be a useful prognostic factor in patients with EBDC. Copyright © 2012 Wiley Periodicals, Inc.

Basement radon entry and stack driven moisture infiltration reduced by active soil depressurization

Treesearch

C.R. Boardman; Samuel V. Glass

2015-01-01

This case study presents measurements of radon and moisture infiltration from soil gases into the basement of an unoccupied research house in Madison, Wisconsin, over two full years. The basement floor and exterior walls were constructed with preservative-treated lumber and plywood. In addition to continuous radon monitoring, measurements included building air...

14. View toward the northwest corner of the basement in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. View toward the northwest corner of the basement in the north segment of the building. Portions of the basement floor are earth, and portions are concrete. For some undetermined reason an unbonded, narrow panel of brick occurs in the west (left) wall. A corbeled brick footing is seen under this panel, as if the panel is carrying a concentrated load. An identical element occurs to the left, outside the camera's view. These 'columns' may support the second-story brick facade over the ground floor store windows. Credit GADA/MRM. - Stroud Building, 31-33 North Central Avenue, Phoenix, Maricopa County, AZ

The role of discrete intrabasement shear zones during multiphase continental rifting

NASA Astrophysics Data System (ADS)

Phillips, Thomas B.; Jackson, Christopher A.-L.; Bell, Rebecca E.; Duffy, Oliver B.; Fossen, Haakon

2016-04-01

Rift systems form within areas of relatively weak, heterogeneous lithosphere, containing a range of pre-existing structures imparted from previous tectonic events. The extent to which these structures may reactivate during later rift phases, and therefore affect the geometry and evolution of superposed rift systems, is poorly understood. The greatest obstacle to understanding how intrabasement structures influence the overlying rift is obtaining detailed constraints on the origin and 3D geometry of structures within crystalline basement. Such structures are often deeply buried beneath rift systems and therefore rarely sampled directly. In addition, due to relatively low internal acoustic impedance contrasts and large burial depths, crystalline basement typically appears acoustically transparent on seismic reflection data showing no resolvable internal structure. However, offshore SW Norway, beneath the Egersund Basin, intrabasement structures are exceptionally well-imaged due to large impedance contrasts within a highly heterogeneous and shallow basement. We use borehole-constrained 2D and 3D seismic reflection data to constrain the 3D geometry of these intrabasement reflections, and examine their interactions with the overlying rift system. Two types of intrabasement structure are observed: (i) thin (c. 100 m) reflections displaying a characteristic trough-peak-trough wavetrain; and (ii) thick (c. 1 km), sub-parallel reflection packages dipping at c. 30°. Through 1D waveform modelling we show that these reflection patterns arise from a layered sequence as opposed to a single interface. Integrating this with our seismic mapping we correlate these structures to the established onshore geology; specifically layered mylonites associated with the Caledonian thrust belt and cross-cutting extensional Devonian shear zones. We observe multiple phases of reactivation along these structures throughout multiple rift events, in addition to a range of interactions with

Indoors and health: results of a systematic literature review assessing the potential health effects of living in basements.

PubMed

Mezzoiuso, Angelo Giosué; Gola, Marco; Rebecchi, Andrea; Riccò, Matteo; Capolongo, Stefano; Buffoli, Maddalena; Tirani, Marcello; Odone, Anna; Signorelli, Carlo

2017-10-23

A new law approved in March 2017 in the Lombardy Region makes it possible to live in basements. Basements are defined as buildings partly below curb level but with at least one-half of its height above the curb. Basements' features and structural characteristics might pose risks to human health. In this paper we adopt a multidisciplinary approach to assess the potential health effects of living in basements. In particular, we define a conceptual framework to describe basements' structural characteristics which are risk factors, as well as the mechanisms through which they impact on human health. We also conduct a systematic review on the scientific databases PubMed,Embase, DOAJ, Proquest and EBSCO to retrieve, pool and critically analyze all available research that quantified the risk of living in basements for different health outcomes. Available evidence suggests living in basements increases the risk of respiratory diseases (asthma and allergic disorders); more heterogeneous data are available for cancers and cardiovascular diseases. As more quantitative data need to be prospectively retrieved to assess and monitor the risk of living in basements for human health, clear minimum requirements for light, air, sanitation and egress are to be defined by technical experts and enforced by policy makers.

Fault zone characteristics and basin complexity in the southern Salton Trough, California

USGS Publications Warehouse

Persaud, Patricia; Ma, Yiran; Stock, Joann M.; Hole, John A.; Fuis, Gary S.; Han, Liang

2016-01-01

Ongoing oblique slip at the Pacific–North America plate boundary in the Salton Trough produced the Imperial Valley (California, USA), a seismically active area with deformation distributed across a complex network of exposed and buried faults. To better understand the shallow crustal structure in this region and the connectivity of faults and seismicity lineaments, we used data primarily from the Salton Seismic Imaging Project to construct a three-dimensional P-wave velocity model down to 8 km depth and a velocity profile to 15 km depth, both at 1 km grid spacing. A VP = 5.65–5.85 km/s layer of possibly metamorphosed sediments within, and crystalline basement outside, the valley is locally as thick as 5 km, but is thickest and deepest in fault zones and near seismicity lineaments, suggesting a causative relationship between the low velocities and faulting. Both seismicity lineaments and surface faults control the structural architecture of the western part of the larger wedge-shaped basin, where two deep subbasins are located. We estimate basement depths, and show that high velocities at shallow depths and possible basement highs characterize the geothermal areas.

Colorectal laterally spreading tumors show characteristic expression of cell polarity factors, including atypical protein kinase C λ/ι, E-cadherin, β-catenin and basement membrane component.

PubMed

Ichikawa, Yasushi; Nagashima, Yoji; Morioka, Kaori; Akimoto, Kazunori; Kojima, Yasuyuki; Ishikawa, Takashi; Goto, Ayumu; Kobayashi, Noritoshi; Watanabe, Kazuteru; Ota, Mitsuyoshi; Fujii, Shoichi; Kawamata, Mayumi; Takagawa, Ryo; Kunizaki, Chikara; Takahashi, Hirokazu; Nakajima, Atsushi; Maeda, Shin; Shimada, Hiroshi; Inayama, Yoshiaki; Ohno, Shigeo; Endo, Itaru

2014-09-01

Colorectal flat-type tumors include laterally spreading tumors (LSTs) and flat depressed-type tumors. The former of which shows a predominant lateral spreading growth rather than an invasive growth. The present study examined the morphological characteristics of LSTs, in comparison with polypoid- or flat depressed-type tumors, along with the expression of atypical protein kinase C (aPKC) λ/ι, a pivotal cell polarity regulator, and the hallmarks of cell polarity, as well as with type IV collagen, β-catenin and E-cadherin. In total, 37 flat-type (24 LSTs and 13 flat depressed-type tumors) and 20 polypoid-type colorectal tumors were examined. The LSTs were classified as 15 LST adenoma (LST-A) and nine LST cancer in adenoma (LST-CA). An immunohistochemical examination was performed on aPKC λ/ι, type IV collagen, β-catenin and E-cadherin. The LST-A and -CA showed a superficial replacing growth pattern, with expression of β-catenin and E-cadherin in the basolateral membrane and type IV collagen along the basement membrane. In addition, 86.6% of LST-A and 55.6% of LST-CA showed aPKC λ/ι expression of 1+ (weak to normal intensity staining in the cytoplasm compared with the normal epithelium). Furthermore, ~45% of the polypoid-type adenomas showed 2+ (moderate intensity staining in the cytoplasm and/or nucleus) and 66.7% of the polypoid-type cancer in adenoma were 3+ (strong intensity staining in the cytoplasm and nucleus). A statistically significant positive correlation was observed between the expression of aPKC λ/ι and β-catenin (r=0.842; P<0.001), or type IV collagen (r=0.823; P<0.001). The LSTs showed a unique growth pattern, different from the expanding growth pattern presented by a polypoid tumor and invasive cancer. The growth characteristics of LST appear to be caused by adequate coexpression of β-catenin, type IV collagen and aPKC λ/ι.

Volcanic conduit migration over a basement landslide at Mount Etna (Italy).

PubMed

Nicolosi, I; Caracciolo, F D'Ajello; Branca, S; Ventura, G; Chiappini, M

2014-06-13

The flanks of volcanoes may slide in response to the loading of the edifice on a weak basement, magma push, and/or to tectonic stress. However, examples of stratovolcanoes emplaced on active landslides are lacking and the possible effects on the volcano dynamics unknown. Here, we use aeromagnetic data to construct a three-dimensional model of the clay-rich basement of Etna volcano (Italy). We provide evidence for a large stratovolcano growing on a pre-existing basement landslide and show that the eastern Etna flank, which slides toward the sea irrespective of volcanic activity, moves coherently with the underlying landslide. The filling of the landslide depression by lava flows through time allows the formation of a stiffness barrier, which is responsible for the long-term migration of the magma pathways from the coast to the present-day Etna summit. These unexpected results provide a new interpretation clue on the causes of the volcanic instability processes and of the mechanisms of deflection and migration of volcanic conduits.

Basement geology of the National Petroleum Reserve Alaska (NPRA), Northern Alaska

USGS Publications Warehouse

Saltus, R.W.; Hudson, T.L.; Phillips, J.D.; Kulander, C.; Dumoulin, Julie A.; Potter, C.

2002-01-01

Gravity, aeromagnetic, seismic, and borehole information enable mapping of crustal basement characteristics within the National Petroleum Reserve Alaska (NPRA). In general, the pre-Mississippian basement of the southern portion of the NPRA is different from that in the north in that it is deeper and thinner, is made up of dense magnetic rocks, is cut by more normal faults, and underlies thicker accumulations of Mississippian to Triassic Ellesmerian sequence sedimentary rocks. Mafic igneous rocks within the basement and locally within the deeper Ellesmerian sequence sedimentary section could explain the observed density and magnetic variations. Because these variations spatially overlap thicker Ellesmerian sequence sediment accumulations, they may have developed, at least in part, during Mississippian to Triassic extension and basin formation. If this period of extension, and postulated mafic magmatism, was accompanied by higher heat flow, then early Ellesmerian sequence clastic sediments may have become mature for hydrocarbon generation (Magoon and Bird, 1988). This could have produced an early petroleum system in the Colville basin.

Origin of a major cross-element zone: Moroccan Rif

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morley, C.K.

1987-08-01

Alpine age (Oligocene-Miocene) deformation in the western Mediterranean formed the Rif mountain belt of northern Morocco. A linear east-northeast-west-southwest trend of cross elements from Jebah (Mediterranean coast) to Arbaoua (near the Atlantic coast) extends through several thrust sheets in the western Rif. The cross elements are manifest as a lateral ramp, the northern limit of a large culmination, and they affect syntectonic turbidite sandstone distribution. Gravity anomalies indicate that the cross-element zone is coincident with a transition zone from normal thickness to thinner continental crust. It is suggested that an early Mesozoic strike-slip fault system related to rifting of Northmore » America from North Africa caused a strong east-northeast-west-southwest, basement block-fault trend to form on the normal thickness side of the thick-to-thin continental crustal transition zone. This trend later influenced the position of the Alpine age cross-element zone that traverses several different Mesozoic and Tertiary basins, inverted during the Alpine deformation.« less

Influence of the Amlia fracture zone on the evolution of the Aleutian Terrace forearc basin, central Aleutian subduction zone

USGS Publications Warehouse

Ryan, Holly F.; Draut, Amy E.; Keranen, Katie M.; Scholl, David W.

2012-01-01

During Pliocene to Quaternary time, the central Aleutian forearc basin evolved in response to a combination of tectonic and climatic factors. Initially, along-trench transport of sediment and accretion of a frontal prism created the accommodation space to allow forearc basin deposition. Transport of sufficient sediment to overtop the bathymetrically high Amlia fracture zone and reach the central Aleutian arc began with glaciation of continental Alaska in the Pliocene. As the obliquely subducting Amlia fracture zone swept along the central Aleutian arc, it further affected the structural evolution of the forearc basins. The subduction of the Amlia fracture zone resulted in basin inversion and loss of accommodation space east of the migrating fracture zone. Conversely, west of Amlia fracture zone, accommodation space increased arcward of a large outer-arc high that formed, in part, by a thickening of arc basement. This difference in deformation is interpreted to be the result of a variation in interplate coupling across the Amlia fracture zone that was facilitated by increasing subduction obliquity, a change in orientation of the subducting Amlia fracture zone, and late Quaternary intensification of glaciation. The change in coupling is manifested by a possible tear in the subducting slab along the Amlia fracture zone. Differences in coupling across the Amlia fracture zone have important implications for the location of maximum slip during future great earthquakes. In addition, shaking during a great earthquake could trigger large mass failures of the summit platform, as evidenced by the presence of thick mass transport deposits of primarily Quaternary age that are found in the forearc basin west of the Amlia fracture zone.

Evaluation of human amniotic membrane as a wound dressing for split-thickness skin-graft donor sites.

PubMed

Loeffelbein, Denys J; Rohleder, Nils H; Eddicks, Matthias; Baumann, Claudia M; Stoeckelhuber, Mechthild; Wolff, Klaus-D; Drecoll, Enken; Steinstraesser, Lars; Hennerbichler, Simone; Kesting, Marco R

2014-01-01

Human amniotic membrane (HAM) has been used as a biomaterial in various surgical procedures and exceeds some qualities of common materials. We evaluated HAM as wound dressing for split-thickness skin-graft (STSG) donor sites in a swine model (Part A) and a clinical trial (Part B). Part A: STSG donor sites in 4 piglets were treated with HAM or a clinically used conventional polyurethane (PU) foil (n = 8 each). Biopsies were taken on days 5, 7, 10, 20, 40, and 60 and investigated immunohistochemically for alpha-smooth muscle actin (αSMA: wound contraction marker), von Willebrand factor (vWF: angiogenesis), Ki-67 (cell proliferation), and laminin (basement membrane integrity). Part B: STSG donor sites in 45 adult patients (16 female/29 male) were treated with HAM covered by PU foam, solely by PU foam, or PU foil/paraffin gauze (n = 15 each). Part A revealed no difference in the rate of wound closure between groups. HAM showed improved esthetic results and inhibitory effects on cicatrization. Angioneogenesis was reduced, and basement membrane formation was accelerated in HAM group. Part B: no difference in re-epithelialization/infection rate was found. HAM caused less ichor exudation and less pruritus. HAM has no relevant advantage over conventional dressings but might be a cost-effective alternative.

New Constraints on the Extent of Paleoproterozoic and Archean Basement in the Northwest U.S. Cordillera

NASA Astrophysics Data System (ADS)

Brewer, R. A.; Vervoort, J.; Lewis, R. S.; Gaschnig, R. M.; Hart, G.

2008-12-01

The Laurentian basement west of the Wyoming craton in southwest Montana and northern Idaho has been interpreted as a collage of Archean and Proterozoic terranes which accreted to the North American craton and incorporated into Laurentia at ~ 1.86 Ga [1]. This basement and the geometry of the Archean and Proterozoic crust are poorly understood due to coverage by metasediments of the Belt-Purcell Supergroup and are further obscured by Mesozoic magmatism (Idaho Batholith, sensu lato). Exposures of the basement are rare but have been documented in a few regions including the Priest River Complex in northern Idaho and the Sevier fold and thrust belt just northwest of the Wyoming craton in the Great Falls tectonic zone (Foster et al. 2006). New ages and isotopic data from orthogneisses in north-central Idaho provide evidence for previously undocumented exposures of both Paleoproterozoic and Archean basement that may place important constraints on the reconstruction of Laurentia and its tectonic setting. The orthogneisses analyzed in this study (all previously mapped as deformed Cretaceous plutons) fall into two distinct age groups of 1.86 Ga and 2.67 Ga. The zircons from both the Archean and Proterozoic rocks have simple systematics. The zircons from three Archean samples have ɛHf(i) values of 2.4 ± 2.1, 3.8 ± 1.8, and 5.2 ± 3.5 (average values based on 6 individual zircon Hf analyses per sample). Zircons from the Paleoproterozoic gneisses have different but internally consistent ɛHf(i) values of -8.0 ± 0.9 and -0.6 ± 1.4. In contrast, both Hf and Nd whole rock data are highly scattered in these samples especially in the Archean samples in which ɛHf(i) varies from -25 to +21 and ɛNd(i) varies from -8 to +11. These extreme values are implausible for initial compositions and indicate open system behavior in both Lu-Hf and Sm-Nd in the whole rocks. The zircons, in contrast, appear to be closed to significant Hf mobility on the scale of the laser analyses. The data

Volcanic conduit migration over a basement landslide at Mount Etna (Italy)

PubMed Central

Nicolosi, I.; Caracciolo, F. D'Ajello; Branca, S.; Ventura, G.; Chiappini, M.

2014-01-01

The flanks of volcanoes may slide in response to the loading of the edifice on a weak basement, magma push, and/or to tectonic stress. However, examples of stratovolcanoes emplaced on active landslides are lacking and the possible effects on the volcano dynamics unknown. Here, we use aeromagnetic data to construct a three-dimensional model of the clay-rich basement of Etna volcano (Italy). We provide evidence for a large stratovolcano growing on a pre-existing basement landslide and show that the eastern Etna flank, which slides toward the sea irrespective of volcanic activity, moves coherently with the underlying landslide. The filling of the landslide depression by lava flows through time allows the formation of a stiffness barrier, which is responsible for the long-term migration of the magma pathways from the coast to the present-day Etna summit. These unexpected results provide a new interpretation clue on the causes of the volcanic instability processes and of the mechanisms of deflection and migration of volcanic conduits. PMID:24924784

Basement structure of the United Arab Emirates derived from an analysis of regional gravity and aeromagnetic database

NASA Astrophysics Data System (ADS)

Ali, M. Y.; Fairhead, J. D.; Green, C. M.; Noufal, A.

2017-08-01

Gravity and aeromagnetic data covering the whole territory of the United Arab Emirates (UAE) have been used to evaluate both shallow and deep geological structures, in particular the depth to basement since it is not imaged by seismic data anywhere within the UAE. Thus, the aim has been to map the basement so that its structure can help to assess its control on the distribution of hydrocarbons within the UAE. Power spectrum analysis reveals gravity and magnetic signatures to have some similarities, in having two main density/susceptibility interfaces widely separated in depth such that regional-residual anomaly separation could effectively be undertaken. The upper density/susceptibility interface occurs at a depth of about 1.0 km while the deeper interface varies in depth throughout the UAE. For gravity, this deeper interface is assumed to be due to the combined effect of lateral changes in density structures within the sediments and in depth of basement while for magnetics it is assumed the sediments have negligible susceptibility and the anomalies unrelated to the volcanic/magmatic bodies result from only changes in depth to basement. The power spectrum analysis over the suspect volcanic/magmatic bodies indicates they occur at 5 km depth. The finite tilt-depth and finite local wavenumber methods were used to estimate depth to source and only depths that agree to within 10% of each other were used to generate the depth to basement map. This depth to basement map, to the west of the UAE-Oman Mountains, varies in depth from 5 km to in excess of 15 km depth and is able to structurally account for the location of the shear structures, seen in the residual magnetic data, and the location of the volcanic/magmatic centres relative to a set of elongate N-S to NE-SW trending basement highs. The majority of oilfields in the UAE are located within these basement highs. Therefore, the hydrocarbon distribution in the UAE basin appears to be controlled by the location of the

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

PubMed Central

Rodriguez-Teja, Mercedes; Breit, Claudia; Clarke, Mitchell; Talar, Kamil; Wang, Kai; Mohammad, Mohammad A.; Pickwell, Sage; Etchandy, Guillermina; Stasiuk, Graeme J.; Sturge, Justin

2016-01-01

Here we describe a protocol that can be used to study the biophysical microenvironment related to increased thickness and stiffness of the basement membrane (BM) during age-related pathologies and metabolic disorders (e.g. cancer, diabetes, microvascular disease, retinopathy, nephropathy and neuropathy). The premise of the model is non-enzymatic crosslinking of reconstituted BM (rBM) matrix by treatment with glycolaldehyde (GLA) to promote advanced glycation endproduct (AGE) generation via the Maillard reaction. Examples of laboratory techniques that can be used to confirm AGE generation, non-enzymatic crosslinking and increased stiffness in GLA treated rBM are outlined. These include preparation of native rBM (treated with phosphate-buffered saline, PBS) and stiff rBM (treated with GLA) for determination of: its AGE content by photometric analysis and immunofluorescent microscopy, its non-enzymatic crosslinking by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) as well as confocal microscopy, and its increased stiffness using rheometry. The procedure described here can be used to increase the rigidity (elastic moduli, E) of rBM up to 3.2-fold, consistent with measurements made in healthy versus diseased human prostate tissue. To recreate the biophysical microenvironment associated with the aging and diseased prostate gland three prostate cell types were introduced on to native rBM and stiff rBM: RWPE-1, prostate epithelial cells (PECs) derived from a normal prostate gland; BPH-1, PECs derived from a prostate gland affected by benign prostatic hyperplasia (BPH); and PC3, metastatic cells derived from a secondary bone tumor originating from prostate cancer. Multiple parameters can be measured, including the size, shape and invasive characteristics of the 3D glandular acini formed by RWPE-1 and BPH-1 on native versus stiff rBM, and average cell length, migratory velocity and persistence of cell movement of 3D spheroids formed by PC3 cells under

Geologic map of the Kechumstuk fault zone in the Mount Veta area, Fortymile mining district, east-central Alaska

USGS Publications Warehouse

Day, Warren C.; O’Neill, J. Michael; Dusel-Bacon, Cynthia; Aleinikoff, John N.; Siron, Christopher R.

2014-01-01

This map was developed by the U.S. Geological Survey Mineral Resources Program to depict the fundamental geologic features for the western part of the Fortymile mining district of east-central Alaska, and to delineate the location of known bedrock mineral prospects and their relationship to rock types and structural features. This geospatial map database presents a 1:63,360-scale geologic map for the Kechumstuk fault zone and surrounding area, which lies 55 km northwest of Chicken, Alaska. The Kechumstuk fault zone is a northeast-trending zone of faults that transects the crystalline basement rocks of the Yukon-Tanana Upland of the western part of the Fortymile mining district. The crystalline basement rocks include Paleozoic metasedimentary and metaigneous rocks as well as granitoid intrusions of Triassic, Jurassic, and Cretaceous age. The geologic units represented by polygons in this dataset are based on new geologic mapping and geochronological data coupled with an interpretation of regional and new geophysical data collected by the Alaska Department of Natural Resources, Division of Geological and Geophysical Surveys. The geochronological data are reported in the accompanying geologic map text and represent new U-Pb dates on zircons collected from the igneous and metaigneous units within the map area.

4. BASEMENT WALL BENEATH SOUTH PASSAGE SHOWING STEAM TUNNEL OPENING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. BASEMENT WALL BENEATH SOUTH PASSAGE SHOWING STEAM TUNNEL OPENING IN SOUTH WALL. - Pennsylvania Railroad Station, South Baggage Passage & Canopy, 1101 Liberty Avenue, Pittsburgh, Allegheny County, PA

7. Details of northeast elevation, demonstrating the relationship of basement ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Details of northeast elevation, demonstrating the relationship of basement windows to column bases. - Roanoke Veterans Administration Hospital, Building No. 6, 1970 Roanoke Boulevard, Salem, Salem, VA

Basement characterization and crustal structure beneath the Arabia-Eurasia collision (Iran): A combined gravity and magnetic study

NASA Astrophysics Data System (ADS)

Mousavi, Naeim; Ebbing, Jörg

2018-04-01

We present a study on the depth to basement and magnetic crustal domains beneath the Iranian Plateau by modeling aeromagnetic and gravity data. First, field processing of the aeromagnetic data was undertaken to estimate the general characteristics of the magnetic basement. Afterwards, inverse modeling of aeromagnetic data was carried out to estimate the depth to basement. The obtained model of basement was refined using combined gravity and magnetic forward modeling. Hereby, we were able to distinguish different magnetic domains in the uppermost crust (10-20 km depths) influencing the medium to long wavelength trends of the magnetic anomalies. Magnetic basement mapping shows that prominent shallow magnetic features are furthermore located in the volcanic areas, e.g. the Urumieh Dokhtar Magmatic Assemblage. The presence of ophiolite outcrops in SE Iran implies that shallow oceanic crust (with high magnetization) is the main source of one of the biggest magnetic anomalies in entire Iran area located north of the Makran.

The Dandridge-Vonore Fault Zone in the Eastern Tennessee Seismic Zone (and Rejuvenation of the Smokies?)

NASA Astrophysics Data System (ADS)

Cox, R. T.; Hatcher, R. D., Jr.; Forman, S. L.; Gamble, E. D. S.; Warrell, K. F.

2017-12-01

The eastern Tennessee seismic zone (ETSZ) trends 045o from NE Alabama and NW Georgia through Tennessee to SE Kentucky, and seismicity is localized 5-26 km deep in the basement. The ETSZ is the second most seismically active region in North America east of the Rocky Mountains, although no historic earthquakes larger than Mw 4.8 have been recorded here. Late Quaternary paleoiseismic evidence suggests that the ETSZ is capable of M7+ earthquakes and that neotectonic faults may have significantly influenced the regional relief. We have identified an 80 km-long, 060o-trending corridor in eastern Tennessee that contains collinear northeast-striking thrust, strike-slip, and normal Quaternary faults with displacements of 1-2 m, herein termed the Dandridge-Vonore fault zone (DVFZ). French Broad River alluvium in the northeast DVFZ near Dandridge, TN, is displaced by a 050o-striking, SE-dipping thrust fault and by a set of related fissures that record at least two significant post 25 ka paleo-earthquakes. Southwest of Dandridge near Alcoa, TN, a 060o-striking, SE-dipping thrust fault cuts Little River alluvium and records two significant post-15 ka paleo-earthquakes. Farther southwest at Vonore, colluvium with alluvial cobbles is thrust >1 m by a 057o-striking, steeply SE-dipping fault that may also have a significant strike-slip component, and Little Tennessee River alluvium is dropped >2 m along a 070o- striking normal fault. The DVFZ partly overlaps and is collinear with a local trend of maximum seismicity that extends 30 km farther SW of the DVFZ (as currently mapped), for a total length of 110 km. The DVFZ is coincident with a steep gradient in S-wave velocities (from high velocity on the SE to low velocity on the NW) at mid-crustal depths of 20 to 24 km, consistent with a fault and source zone at hypocentral depths in the crystalline basement. Moreover, the DVFZ parallels the NW foot of Blue Ridge Mountains, and the sense of thrusting at all sites of Quaternary faulting

Organic membranous skeleton of the Precambrian metazoans from Namibia

NASA Astrophysics Data System (ADS)

Dzik, Jerzy

1999-06-01

Unlike the celebrated Ediacara fossils, those from the roughly coeval localities of the Kuibis Quarzite of Namibia are preserved not as imprints on the sandstone bedding plane, but three-dimensionally, within the rock matrix. The pattern of deformation and the presence of sand in lower parts of the bodies of Ernietta, the most common and typical of those organisms, indicate that their three-dimensional preservation is a result of a density-controlled sinking of sand-filled organic skeletons within hydrated mud layers. Specimens of Ernietta have preserved various stages of migration across the mud beds. Their wall material, as documented by the mode of deformation, was not only flexible, but also elastic, which makes it unlike chitin. The walls thus seem to be proteinaceous, built probably of a collagenous fabric. The Ernietta skeleton was built of series of parallel chambers, which excludes the possibility that these were external body covers. The chambers apparently represent walls of hydraulic skeleton units, resembling the basement membrane of chaetognaths or the notochord sheath of primitive chordates. Such chambers are widespread among the earliest fossil animals represented by fossils preserved in sandstone. The rise and fall of the Ediacaran faunas thus seem to be partially preservational artifacts. The range of its occurrence is a result of two successive evolutionary events: the origin of an internal hydraulic skeleton enclosed by a strong basement membrane, and the appearance of decomposers with abilities to disintegrate such collagenous sheaths.

Membrane-Mediated Cooperativity of Proteins

NASA Astrophysics Data System (ADS)

Weikl, Thomas R.

2018-04-01

Besides direct protein-protein interactions, indirect interactions mediated by membranes play an important role for the assembly and cooperative function of proteins in membrane shaping and adhesion. The intricate shapes of biological membranes are generated by proteins that locally induce membrane curvature. Indirect curvature-mediated interactions between these proteins arise because the proteins jointly affect the bending energy of the membranes. These curvature-mediated interactions are attractive for crescent-shaped proteins and are a driving force in the assembly of the proteins during membrane tubulation. Membrane adhesion results from the binding of receptor and ligand proteins that are anchored in the apposing membranes. The binding of these proteins strongly depends on nanoscale shape fluctuations of the membranes, leading to a fluctuation-mediated binding cooperativity. A length mismatch between receptor-ligand complexes in membrane adhesion zones causes repulsive curvature-mediated interactions that are a driving force for the length-based segregation of proteins during membrane adhesion.

A Nonlinear Inversion Approach to Map the Magnetic Basement: A Case Study from Central India Using Aeromagnetic Data

NASA Astrophysics Data System (ADS)

Kumar, R.; Bansal, A. R.; Anand, S. P.; Rao, V. K.; Singh, U. K.

2016-12-01

The central India region is having complex geology covering various geological units e.g., Precambrian Bastar Craton (including Proterozoic Chhattisgarh Basin, granitic intrusions etc.) and Eastern Ghat Mobile Belt, Gondwana Godavari and Mahanadi Grabens, Late Cretaceous Deccan Traps etc. The central India is well covered by reconnaissance scale aeromagnetic data. We analyzed this data for mapping the basement by dividing into143 overlapping blocks of 100×100km using least square nonlinear inversion method for fractal distribution of sources. The scaling exponents and depth values are optimized using grid search method. We interpreted estimated depths of anomalous sources as magnetic basement and shallow anomalous magnetic sources. The shallow magnetic anomalies are found to vary from 1 to 3km whereas magnetic basement depths are found to vary from 2km to 7km. The shallowest basement depth of 2km found corresponding to Kanker granites a part of Bastar Craton whereas deepest basement depth of 7km is associated with Godavari Graben and south eastern part of Eastern Ghat Mobile Belts near the Parvatipuram Bobbili fault. The variation of magnetic basement, shallow depths and scaling exponent in the region indicate complex tectonic, heterogeneity and intrusive bodies at different depths which is due to different tectonic processes in the region. The detailed basement depth of central India is presented in this study.

Paleozoic to early Cenozoic cooling and exhumation of the basement underlying the eastern Puna plateau margin prior to plateau growth

NASA Astrophysics Data System (ADS)

Insel, N.; Grove, M.; Haschke, M.; Barnes, J. B.; Schmitt, A. K.; Strecker, M. R.

2012-12-01

Constraining the pre-Neogene history of the Puna plateau is crucial for establishing the initial conditions that attended the early stage evolution of the southern extent of the Andean plateau. We apply high- to low-temperature thermochronology data from plutonic rocks in northwestern Argentina to quantify the Paleozoic, Mesozoic and early Tertiary cooling history of the Andean crust. U-Pb crystallization ages of zircons indicate that pluton intrusion occurred during the early mid-Ordovician (490-470 Ma) and the late Jurassic (160-150 Ma). Lower-temperature cooling histories from 40Ar/39Ar analyses of K-feldspar vary substantially. Basement rocks underlying the western Puna resided at temperatures below 200°C (<6 km depth) since the Devonian (˜400 Ma). In contrast, basement rocks underlying the southeastern Puna were hotter (˜200-300°C) throughout the Paleozoic and Jurassic and cooled to temperatures of <200°C by ˜120 Ma. The southeastern Puna basement records a rapid cooling phase coeval with active extension of the Cretaceous Salta rift at ˜160-100 Ma that we associate with tectonic faulting and lithospheric thinning. The northeastern Puna experienced protracted cooling until the late Cretaceous with temperatures <200°C during the Paleocene. Higher cooling rates between 78 and 55 Ma are associated with thermal subsidence during the postrift stage of the Salta rift and/or shortening-related flexural subsidence. Accelerated cooling and deformation during the Eocene was focused within a narrow zone along the eastern Puna/Eastern Cordillera transition that coincides with Paleozoic/Mesozoic structural and thermal boundaries. Our results constrain regional erosion-induced cooling throughout the Cenozoic to have been less than ˜150°C, which implies total Cenozoic denudation of <6-4 km.

The Eastern Tennessee Seismic Zone: Reactivation of an Ancient Continent-Continent Suture Zone

NASA Astrophysics Data System (ADS)

Powell, C. A.

2014-12-01

The eastern Tennessee seismic zone (ETSZ) may represent reactivation of an ancient shear zone that accommodated left-lateral, transpressive motion of the Amazon craton during the Grenville orogeny. Several different lines of evidence support this concept including velocity models for the crust, earthquake hypocenter alignments, focal mechanism solutions, potential field anomalies, paleomagnetic pole positions, and isotopic geochemical studies. The ETSZ trends NE-SW for about 300 km and displays remarkable correlation with the prominent New York - Alabama (NY-AL) aeromagnetic lineament. Vp and Vs models for the crust derived from a local ETSZ earthquake tomography study reveal the presence of a narrow, NE-SW trending, steeply dipping zone of low velocities that extends to a depth of at least 24 km and is associated with the vertical projection of the NY-AL aeromagnetic lineament. The low velocity zone is interpreted as a major basement fault. The recent Mw 4.2 Perry County eastern Kentucky earthquake occurred north of the ETSZ but has a focal depth and mechanism that are similar to those for ETSZ earthquakes. We investigate the possibility that the proposed ancient shear zone extends into eastern Kentucky using Bouguer and aeromagnetic maps. The southern end of the ETSZ is characterized by hypocenters that align along planes dipping at roughly 45 degrees and focal mechanisms that contain large normal faulting components. The NY-AL aeromagnetic lineament also changes trend in the southern end of the ETSZ and the exact location of the lineament is ambiguous. We suggest that the southern portion of the ETSZ involves reactivation of reverse faults (now as normal faults) that mark the ancient transition between a collisional to a more transpressive boundary between Amazonia and Laurentia during the formation of the super continent Rodinia.

4. VIEW LOOKING WEST FROM BASEMENT UNDER OPEN CONCOURSE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. VIEW LOOKING WEST FROM BASEMENT UNDER OPEN CONCOURSE - CEILING OF BRICK ARCHES; WOOD PANELS MARK LOCATION OF ORIGINAL GLASS PRISM SKYLIGHTS REPLACED WITH CONCRETE - Pennsylvania Railroad Station, Open Concourse & Concourse Roof Extension, 1101 Liberty Avenue, Pittsburgh, Allegheny County, PA

Basement thrust sheets in the Clearwater orogenic zone, central Idaho and western Montana

NASA Astrophysics Data System (ADS)

Skipp, Betty

1987-03-01

The Clearwater orogenic zone in central Idaho and western Montana contains at least two major northeast-directed Cordilleran thrust plates of Early Proterozoic metasedimentary and metaigneous rocks that overrode previously folded Middle Proterozoic rocks of the Belt basin in Cretaceous time. The northeastward migration of the resultant thickened wedge of crustal material combined with Cretaceous subduction along the western continental margin produced a younger northern Bitterroot lobe of the Idaho batholith relative to an older southern Atlanta lobe. Eocene extensional unroofing and erosion of the Bitterroot lobe has exposed the roots of the thick Cordilleran thrust sheets.

58. Corridor, building 500 to building 515, basement level, looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

58. Corridor, building 500 to building 515, basement level, looking southeast - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

Basement, bathroom, looking south U.S. Veterans Hospital, Jefferson Barracks, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Basement, bathroom, looking south - U.S. Veterans Hospital, Jefferson Barracks, Medical Officer in Charge Residence, VA Medical Center, Jefferson Barracks Division 1 Jefferson Barracks Drive, Saint Louis, Independent City, MO

Free and membrane-bound calcium in microgravity and microgravity effects at the membrane level

NASA Astrophysics Data System (ADS)

Belyavskaya, N. A.

The changes of [Ca^2+]_i controlled is known to play a key regulatory role in numerous cellular processes especially associated with membranes. Previous studies from our laboratory have demonstrated an increase in calcium level in root cells of pea seedlings grown aboard orbital station ``Salyut 6'' /1/. These results: 1) indicate that observed Ca^2+-binding sites of membranes also consist in proteins and phospholipids; 2) suggest that such effects of space flight in membrane Ca-binding might be due to the enhancement of Ca^2+ influx through membranes. In model presented, I propose that Ca^2+-activated channels in plasma membrane in response to microgravity allow the movement of Ca^2+ into the root cells, causing a rise in cytoplasmic free Ca^2+ levels. The latter, in its turn, may induce the inhibition of a Ca^2+ efflux by Ca^2+-activated ATPases and through a Ca^2+/H^+ antiport. It is possible that increased cytosolic levels of Ca^2+ ions have stimulated hydrolysis and turnover of phosphatidylinositols, with a consequent elevation of cytosolic [Ca^2+]_i. Plant cell can response to such a Ca^2+ rise by an enhancement of membranous Ca^2+-binding activities to rescue thus a cell from an abundance of a cytotoxin. A Ca^2+-induced phase separation of membranous lipids assists to appear the structure nonstable zones with high energy level at the boundary of microdomains which are rich by some phospholipid components; there is mixing of molecules of the membranes contacted in these zones, the first stage of membranous fusion, which was found in plants exposed to microgravity. These results support the hypothesis that a target for microgravity effect is the flux mechanism of Ca^2+ to plant cell.

12. COLD CALIBRATION BLOCKHOUSE BASEMENT VIEW FROM LEFT TO RIGHT, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. COLD CALIBRATION BLOCKHOUSE BASEMENT VIEW FROM LEFT TO RIGHT, CABLE TRAYS, RACKS, CABLE CONNECTION TERMINALS. - Marshall Space Flight Center, East Test Area, Cold Calibration Test Stand, Huntsville, Madison County, AL

STAIRWAY FROM THE PROMENADE TO THE BASEMENT SHOWING SHELTER SIGNAGE. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

STAIRWAY FROM THE PROMENADE TO THE BASEMENT SHOWING SHELTER SIGNAGE. VIEW FACING WEST - U.S. Naval Base, Pearl Harbor, Theater, Hornet Avenue between Enterprise & Pokomoke Streets, Pearl City, Honolulu County, HI

7. BLOCK HOUSE BASEMENT LOOKING THROUGH DOOR INTO CABLE TUNNEL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. BLOCK HOUSE BASEMENT LOOKING THROUGH DOOR INTO CABLE TUNNEL RUNNING BETWEEN BLOCK HOUSE AND STATIC TEST TOWER. - Marshall Space Flight Center, East Test Area, Block House, Huntsville, Madison County, AL

Developmental distribution of the plasma membrane-enriched proteome in the maize primary root growth zone.

PubMed

Zhang, Zhe; Voothuluru, Priyamvada; Yamaguchi, Mineo; Sharp, Robert E; Peck, Scott C

2013-01-01

Within the growth zone of the maize primary root, there are well-defined patterns of spatial and temporal organization of cell division and elongation. However, the processes underlying this organization remain poorly understood. To gain additional insights into the differences amongst the defined regions, we performed a proteomic analysis focusing on fractions enriched for plasma membrane (PM) proteins. The PM is the interface between the plant cell and the apoplast and/or extracellular space. As such, it is a key structure involved in the exchange of nutrients and other molecules as well as in the integration of signals that regulate growth and development. Despite the important functions of PM-localized proteins in mediating these processes, a full understanding of dynamic changes in PM proteomes is often impeded by low relative concentrations relative to total proteins. Using a relatively simple strategy of treating microsomal fractions with Brij-58 detergent to enrich for PM proteins, we compared the developmental distribution of proteins within the root growth zone which revealed a number of previously known as well as novel proteins with interesting patterns of abundance. For instance, the quantitative proteomic analysis detected a gradient of PM aquaporin proteins similar to that previously reported using immunoblot analyses, confirming the veracity of this strategy. Cellulose synthases increased in abundance with increasing distance from the root apex, consistent with expected locations of cell wall deposition. The similar distribution pattern for Brittle-stalk-2-like protein implicates that this protein may also have cell wall related functions. These results show that the simplified PM enrichment method previously demonstrated in Arabidopsis can be successfully applied to completely unrelated plant tissues and provide insights into differences in the PM proteome throughout growth and development zones of the maize primary root.

Paleomagnetism and alteration of lower Paleozoic rocks and Precambrian basement in the SHADS No. 4 drill core, Oklahoma

NASA Astrophysics Data System (ADS)

Evans, S. C.; Hamilton, M.; Hardwick, J.; Terrell, C.; Elmore, R. D.

2017-12-01

The chacterization of the lower Paleozoic sedimentary rock and the underlying Precambrian basement in northern Oklahoma is currently the subject of research to better understand induced seismicity in Oklahoma. We are investigating approximately 140 meters of igneous basement and over 300 meters of Ordovician Arbuckle Group carbonates and underlying sandstone in the Amoco SHADS No. 4 drill core from Rogers Co., Oklahoma, to better understand the nature, origin, and timing of fluid alteration and the relationship between fluid flow in the Arbuckle Group and the basement. Preliminary attempts to orient the core using the viscous remanent magnetization (VRM) method were unsuccessful, probably due to a steep drilling-induced component. The dolomitized Arbuckle Group contains a characteristic remanent magnetization (ChRM) with shallow inclinations (-5°) and variable declinations that, based on unblocking temperatures, is interpreted to reside in magnetite. This ChRM is interpreted as a chemical remanent magnetization (CRM) acquired in the Permian based on the shallow inclinations. The CRM could be related to hydrothermal fluids which migrated into the rocks in the late Paleozoic, as other studies in northern Oklahoma have reported. The Arbuckle Group dolomites are porous and extensively altered and consist of several generations of dolomite, including baroque dolomite. The basement rock is andesitic to trachytic ignimbrite that exhibits extensive alteration. There are many near-vertical fractures mineralized with epidote that are cross cut by calcite-filled fractures. Anisotropy of magnetic susceptibility (AMS) measurements indicate an oblate fabric in the top of the basement and the overlying sandstones. At greater depths, the AMS is variable and may include both alteration and primary fabrics. Demagnetization of the basement rocks is in the initial stages. We are currently investigating if and how far the alteration in the Arbuckle Group extended into the basement

Estimating the Subsurface Basement Topography of Dodge County, Wisconsin Using Three Dimensional Modeling of Gravity and Aeromagnetic Data

NASA Astrophysics Data System (ADS)

MacAlister, E.; Skalbeck, J.; Stewart, E.

2016-12-01

Since the late 1800's, geologic studies have been completed in Wisconsin in pursuit of understanding the basement topography and locating economically viable mineral resources. The doubly plunging Baraboo Syncline located in Columbia and Sauk Counties provides a classic record of Precambrian deformation. A similar buried structure is thought to exist in adjacent Dodge County based on a prominent aeromagnetic anomaly. For this study, 3-D modeling of gravity and aeromagnetic survey data was used to approximate the structure of the Precambrian basement topography beneath Dodge County, Wisconsin. The aim of the research was to determine a suitable basement topography grid using potential field data and then use this grid as the base for groundwater flow models. Geosoft Oasis Montaj GM-SYS 3D modeling software was used to build grids of subsurface layers and the model was constrained by well records of basement rock elevations located throughout the county. The study demonstrated that there is a complex network of crystalline basement structures that have been folded through tectonic activity during the Precambrian. A thick layer of iron rich sedimentary material was deposited on top of the basement rocks, causing a distinct magnetic signature that outlined the basement structure in the magnetic survey. Preliminary results reveal an iron layer with a density of 3.7 g/cm3 and magnetic susceptibility of 8000 x 10-6 cgs that is approximately 500 feet thick and ranges between elevations of -300 meters below and 400 meters above sea level. The 3-D model depths are consistent with depths from recent core drilling operations performed by the Wisconsin Geological and Natural History Survey. Knowing the depth to and structure of basement rock throughout Dodge County and Wisconsin plays an important role in understanding the geologic history of the region. Also, better resolution of the basement topography can enhance the accuracy of future groundwater flow models.

Deep structure of the Texas Gulf passive margin and its Ouachita-Precambrian basement: Results of the COCORP San Marcos arch survey

DOE Office of Scientific and Technical Information (OSTI.GOV)

Culotta, R.; Latham, T.; Oliver, J.

1992-02-01

This COCORP deep seismic survey provides a comprehensive image of the southeast-Texas part of the Gulf passive margin and its accreted Ouachita arc foundation. Beneath the updip limit of the Cenozoic sediment wedge, a prominent antiformal structure is imaged within the interior zone of the buried late Paleozoic Ouachita orogen. The structure appears to involve Precambrian Grenville basement. The crest of the antiform is coincident with the Cretaceous-Tertiary Luling-Mexia-Talco fault zone. Some of these faults dip to the northwest, counter to the general regional pattern of down-to-the-basin faulting, and appear to sole into the top of the antiform, suggesting thatmore » the Ouachita structure has been reactivated as a hingeline to the subsiding passive margin. The antiform may be tied via this fault system and the Ouachita gravity gradient to the similar Devils River, Waco, and Benton uplifts, interpreted as Precambrian basement-cored massifs. Above the Paleozoic sequence, a possible rift-related graben is imaged near the updip limit of Jurassic salt. Paleoshelf edges of the major Tertiary depositional sequences are marked by expanded sections disrupted by growth faults and shale diapirs. Within the Wilcox Formation, the transect crosses the mouth of the 900-m-deep Yoakum Canyon, a principal pathway of sediment delivery from the Laramide belt to the Gulf. Beneath the Wilcox, the Comanchean (Lower Cretaceous) shelf edge, capped by the Stuart City reef, is imaged as a pronounced topographic break onlapped by several moundy sediment packages. Because this segment of the line parallels strike, the topographic break may be interpreted as a 2,000-m-deep embayment in the Cretaceous shelf-edge, and possibly a major submarine canyon older and deeper than the Yoakum Canyon.« less

35. Basement, passage beneath main entrance porch, showing circular skylight ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. Basement, passage beneath main entrance porch, showing circular skylight opening, view to northwest - Portsmouth Naval Hospital, Hospital Building, Rixey Place, bounded by Williamson Drive, Holcomb Road, & The Circle, Portsmouth, Portsmouth, VA

North rear, east part. Ramp leads to basement utility rooms ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

North rear, east part. Ramp leads to basement utility rooms and specimen preparation rooms. - San Bernardino Valley College, Life Science Building, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

21. Generator Room (basement beneath Blacksmith Shop): view looking north ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. Generator Room (basement beneath Blacksmith Shop): view looking north showing remains of 1923 six-cylinder Studebaker engine and a dynamo - Ben Thresher's Mill, State Aid No. 1, Barnet, Caledonia County, VT

17. Woodworking Mill (basement): view looking north showing Ames Iron ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. Woodworking Mill (basement): view looking north showing Ames Iron Works steam boiler; note turbine control handle in middle right of photo - Ben Thresher's Mill, State Aid No. 1, Barnet, Caledonia County, VT

Detail of basement level concrete beams at southwest corner; camera ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail of basement level concrete beams at southwest corner; camera facing west. - Mare Island Naval Shipyard, Hospital Ward, Johnson Lane, west side at intersection of Johnson Lane & Cossey Street, Vallejo, Solano County, CA

17. INTERIOR VIEW OF BASEMENT EXHIBITION OF EVENTS OF CIVIL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. INTERIOR VIEW OF BASEMENT EXHIBITION OF EVENTS OF CIVIL RIGHTS MOVEMENT AND THE 1963 BOMBING OF THE CHURCH, LOOKING SOUTH - Sixteenth Street Baptist Church, 1530 Sixth Avenue North, Birmingham, Jefferson County, AL

Noncatalytic hydrogenation of naphthalene in nanosized membrane reactors with accumulated hydrogen and controlled adjustment of their reaction zone volumes

NASA Astrophysics Data System (ADS)

Soldatov, A. P.

2017-05-01

As part of ongoing studies aimed at designing the next generation of nanosized membrane reactors (NMRs) with accumulated hydrogen, the noncatalytic hydrogenation of naphthalene in pores of ceramic membranes (TRUMEM ultrafiltration membranes with D av = 50 and 90 nm) is performed for the first time, using hydrogen preadsorbed in a hybrid carbon nanostructure: mono- and multilayered oriented carbon nanotubes with graphene walls (OCNTGs) that form on inner pore surfaces. In this technique, the reaction proceeds in the temperature range of 330-390°C at contact times of 10-16 h. The feedstock is an 8% naphthalene solution in decane. The products are analyzed via chromatography on a quartz capillary column coated with polydimethylsiloxane (SE-30). It is established for the first time that in NMRs, the noncatalytic hydrogenation of naphthalene occurs at 370-390°C, forming 1,2,3,4-tetrahydronaphthalene in amounts of up to 0.61%. The rate constants and activation energy (123.5 kJ/mol) of the noncatalytic hydrogenation reaction are determined for the first time. The possibility of designing an NMR with an adjustable reaction zone volume is explored. Changes in the pore structure of the membranes after their modification with pyrocarbon nanosized crystallites (PNCs) are therefore studied as well. It is shown that lengthening the process time reduces pore size: within 23 h after the deposition of PNCs, the average pore radius ( r av) falls from 25 to 3.1 nm. The proposed approach would allow us to design nanoreactors of molecular size and conduct hydrogenation reactions within certain guidelines to synthesize new chemical compounds.

Proteomic analysis of corneal endothelial cell-descemet membrane tissues reveals influence of insulin dependence and disease severity in type 2 diabetes mellitus.

PubMed

Skeie, Jessica M; Aldrich, Benjamin T; Goldstein, Andrew S; Schmidt, Gregory A; Reed, Cynthia R; Greiner, Mark A

2018-01-01

The objective of this study was to characterize the proteome of the corneal endothelial cell layer and its basement membrane (Descemet membrane) in humans with various severities of type II diabetes mellitus compared to controls, and identify differentially expressed proteins across a range of diabetic disease severities that may influence corneal endothelial cell health. Endothelium-Descemet membrane complex tissues were peeled from transplant suitable donor corneas. Protein fractions were isolated from each sample and subjected to multidimensional liquid chromatography and tandem mass spectrometry. Peptide spectra were matched to the human proteome, assigned gene ontology, and grouped into protein signaling pathways unique to each of the disease states. We identified an average of 12,472 unique proteins in each of the endothelium-Descemet membrane complex tissue samples. There were 2,409 differentially expressed protein isoforms that included previously known risk factors for type II diabetes mellitus related to metabolic processes, oxidative stress, and inflammation. Gene ontology analysis demonstrated that diabetes progression has many protein footprints related to metabolic processes, binding, and catalysis. The most represented pathways involved in diabetes progression included mitochondrial dysfunction, cell-cell junction structure, and protein synthesis regulation. This proteomic dataset identifies novel corneal endothelial cell and Descemet membrane protein expression in various stages of diabetic disease. These findings give insight into the mechanisms involved in diabetes progression relevant to the corneal endothelium and its basement membrane, prioritize new pathways for therapeutic targeting, and provide insight into potential biomarkers for determining the health of this tissue.

8. SAC command center underground structure, building 501, basement entry, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. SAC command center underground structure, building 501, basement entry, machine room, April 11, 1955 - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Command Center, 901 SAC Boulevard, Bellevue, Sarpy County, NE

Dissolution of biogenic ooze over basement edifices in the equatorial Pacific with implications for hydrothermal ventilation of the oceanic crust

USGS Publications Warehouse

Bekins, B.A.; Spivack, A.J.; Davis, E.E.; Mayer, L.A.

2007-01-01

Recent observations indicate that curious closed depressions in carbonate sediments overlying basement edifices are widespread in the equatorial Pacific. A possible mechanism for their creation is dissolution by fluids exiting basement vents from off-axis hydrothermal flow. Quantitative analysis based on the retrograde solubility of calcium carbonate and cooling of basement fluids during ascent provides an estimate for the dissolution capacity of the venting fluids. Comparison of the dissolution capacity and fluid flux with typical equatorial Pacific carbonate mass accumulation rates shows that this mechanism is feasible. By maintaining sediment-free basement outcrops, the process may promote widespread circulation of relatively unaltered seawater in the basement in an area where average sediment thicknesses are 300-500 m. The enhanced ventilation can explain several previously puzzling observations in this region, including anomalously low heat flux, relatively unaltered seawater in the basement, and aerobic and nitrate-reducing microbial activity at the base of the sediments. ?? 2007 The Geological Society of America.

FORMATION OF INTRACYTOPLASMIC MEMBRANE SYSTEM OF MYCOBACTERIA RELATED TO CELL DIVISION

PubMed Central

Imaeda, Tamotsu; Ogura, Mituo

1963-01-01

Imaeda, Tamotsu (Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela) and Mitua Ogura. Formation of intracytoplasmic membrane system of mycobacteria related to cell division. J. Bacteriol. 85:150–163. 1963.—Mycobacterium leprae, M. lepraemurium, and a Mycobacterium sp. were observed with an electron microscope. In these bacilli, the three-dimensional structure of the intracytoplasmic membrane system consists of tubular infoldings of the invaginated plasma membrane. The moderately dense substance, presumably representing the cell-wall precursor, is found in the membranous system, especially in the rapid growth phase of mycobacteria. This system always shows an intimate relationship with cell division. A low-density zone, probably corresponding to the low-density substance which coats the cell wall, appears in the connecting regions of the system and in the longitudinal portion of the cell wall. These zones extend centripetally, and the separation of the cell wall occurs after the two zones meet. Based on these results, we hypothesize that the intracytoplasmic membrane system may produce cell-wall material during cell division of mycobacteria. Images PMID:13956365

3D model of Campo de Dalías basement from H/V spectral ratio of ambient seismic noise

NASA Astrophysics Data System (ADS)

García-Jerez, Antonio; Seivane, Helena; Luzón, Francisco; Navarro, Manuel; Molina, Luis; Aranda, Carolina; Piña-Flores, José; Navarro, Francisco; Sánchez-Martos, Francisco; Vidal, Francisco; Posadas, Antonio M.; Sánchez-Sesma, Francisco J.

2017-04-01

Campo de Dalías is a large coastal plain in the southeastern mountain front of the Betic Cordillera (SE of the Iberian Peninsula), being one of the most seismically active regions of Spain. This area has a population of about 213.000 inhabitants, with a high growth rate during the last decades due to the development of intensive agricultural activities. Seismic risk assessment and hydrogeological issues are major topics of interest for this area, relaying on the knowledge of the geophysical properties of the basin. A passive seismic survey has been conducted throughout the basin. We have recorded ambient noise at 340 sites located approximately on the vertexes of a 1000 x 1000 m square grid, as well as around a set of deep boreholes reaching the Triassic basement. These broad-band records, of at least 45 minutes long each, have been analyzed by using the horizontal-to-vertical spectral ratio method (H/V). The spectral analysis shows clear H/V peaks with periods ranging from 0.3 s to 4 s, approximately, associated to relevant contrasts in S-wave velocity (Vs) at depth. Simulations based on the diffuse field approach (Sánchez-Sesma et al. 2011) show that long periods are explained by the effect of several hundred meters of soft sedimentary rocks (mainly Miocene marls). Well-developed high-frequency secondary peaks have been found in some specific zones (e.g. N of Roquetas de Mar town). Then, fundamental frequencies and basement depths at borehole sites have been fitted by means of a power law, which can be applied down to 900 - 970m. Larger depths are estimated by extrapolation. This relationship has been used to map the basement (main Vs contrast) throughout the plain. The prospected basement model describes well the main structural features of this smoothly folded region, namely, the El Ejido Synform and the Guardias Viejas Antiform, with ENE-WSW-trend. These features are shifted toward the south in comparison with Pedrera et al. (2015). The homogeneous

32. VIEW OF BASEMENT BELOW BOILER 904 LOOKING SOUTHEAST AT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

32. VIEW OF BASEMENT BELOW BOILER 904 LOOKING SOUTHEAST AT TURBINE DRIVEN FORCED DRAFT FAN FOR BOILER 904. - New York, New Haven & Hartford Railroad, Cos Cob Power Plant, Sound Shore Drive, Greenwich, Fairfield County, CT

Percolation of diagenetic fluids in the Archaean basement of the Franceville basin

NASA Astrophysics Data System (ADS)

Mouélé, Idalina Moubiya; Dudoignon, Patrick; Albani, Abderrazak El; Cuney, Michel; Boiron, Marie-Christine; Gauthier-Lafaye, François

2014-01-01

The Palaeoproterozoic Franceville basin, Gabon, is mainly known for its high-grade uranium deposits, which are the only ones known to act as natural nuclear fission reactors. Previous work in the Kiéné region investigated the nature of the fluids responsible for these natural nuclear reactors. The present work focuses on the top of the Archaean granitic basement, specifically, to identify and date the successive alteration events that affected this basement just below the unconformity separating it from the Palaeoproterozoic basin. Core from four drill holes crosscutting the basin-basement unconformity have been studied. Dating is based on U-Pb isotopic analyses performed on monazite. The origin of fluids is discussed from the study of fluid inclusion planes (FIP) in quartz from basement granitoids. From the deepest part of the drill holes to the unconformable boundary with the basin, propylitic alteration assemblages are progressively replaced by illite and locally by a phengite + Fe chlorite ± Fe oxide assemblage. Illitic alteration is particularly strong along the sediment-granitoid contact and is associated with quartz dissolution. It was followed by calcite and anhydrite precipitation as fracture fillings. U-Pb isotopic dating outlines three successive events: a 3.0-2.9-Ga primary magmatic event, a 2.6-Ga propylitic alteration and a late 1.9-Ga diagenetic event. Fluid inclusion microthermometry suggests the circulation of three types of fluids: (1) a Na-Ca-rich diagenetic brine, (2) a moderately saline (diagenetic + meteoric) fluid, and (3) a low-salinity fluid of probable meteoric origin. These fluids are similar to those previously identified within the overlying sedimentary rocks of the Franceville basin. Overall, the data collected in this study show that the Proterozoic-Archaean unconformity has operated as a major flow corridor for fluids circulation, around 1.9 Ga. highly saline diagenetic brines; hydrocarbon-rich fluids derived from organic matter

36. Basement, cellar beneath main stairway, with skylight in stair ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

36. Basement, cellar beneath main stairway, with skylight in stair riser, and adjacent storage room, view to east - Portsmouth Naval Hospital, Hospital Building, Rixey Place, bounded by Williamson Drive, Holcomb Road, & The Circle, Portsmouth, Portsmouth, VA

12. Credit WS. Basement of Mill, showing wooden flywheels to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

12. Credit WS. Basement of Mill, showing wooden flywheels to water wheel and Fairbanks. Morse 20 hp, 350 rpm diesel engine, patented April 20, 1920. - Bunker Hill Mill, County Route 26, Bunker Hill, Berkeley County, WV

26. OVERALL SHOT OF BASEMENT, MILL NO. 1. ORIGINALLY MACHINE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. OVERALL SHOT OF BASEMENT, MILL NO. 1. ORIGINALLY MACHINE SHOP. PALLETS ON FLOOR ADDED IN LATE 20th C. FOR CLOTH STORAGE. - Prattville Manufacturing Company, Number One, 242 South Court Street, Prattville, Autauga County, AL

Gravitational sliding of the Mt. Etna massif along a sloping basement

NASA Astrophysics Data System (ADS)

Murray, John B.; van Wyk de Vries, Benjamin; Pitty, Andy; Sargent, Phil; Wooller, Luke

2018-04-01

Geological field evidence and laboratory modelling indicate that volcanoes constructed on slopes slide downhill. If this happens on an active volcano, then the movement will distort deformation data and thus potentially compromise interpretation. Our recent GPS measurements demonstrate that the entire edifice of Mt. Etna is sliding to the ESE, the overall direction of slope of its complex, rough sedimentary basement. We report methods of discriminating the sliding vector from other deformation processes and of measuring its velocity, which averaged 14 mm year-1 during four intervals between 2001 and 2012. Though sliding of one sector of a volcano due to flank instability is widespread and well-known, this is the first time basement sliding of an entire active volcano has been directly observed. This is important because the geological record shows that such sliding volcanoes are prone to devastating sector collapse on the downslope side, and whole volcano migration should be taken into account when assessing future collapse hazard. It is also important in eruption forecasting, as the sliding vector needs to be allowed for when interpreting deformation events that take place above the sliding basement within the superstructure of the active volcano, as might occur with dyke intrusion or inflation/deflation episodes.

A classification of morphoseismic features in the New Madrid seismic zone

DOE Office of Scientific and Technical Information (OSTI.GOV)

Knox, R.; Stewart, D.

1993-03-01

The New Madrid Seismic Zone (NMSZ) contains thousands of surface features distributed over 5,000 square miles in four states. These are attributable to some combination of (1) seismically-induced liquefaction (SIL), (2) secondary deformation, and (3) seismically-induced slope failures. Most of these features were produced by the 1811--12 series of great earthquakes, but some predate and some postdate 1811--12. Subsequent non-seismic factors, such as hydrologically-induced liquefaction (HIL), mechanically-induced liquefaction (MIL), human activities, mass wasting, eolian and fluvial processes have modified all of these features. Morphoseismic features are new landforms produced by earthquakes, or are pre-existing landforms modified by them. Involved aremore » complex interrelationships among several variables, including: (1) intensity and duration of seismic ground motion, (2) surface wave harmonics, (3) depth to water table, (4) depth to basement, (5) particle size, composition, and sorting of sediment making up the liquefied (LZ) and non-liquefied zones (NLZ), (6) topographic parameters, and (7) attitudes of beds and lenses susceptible to liquefaction. Morphoseismic features are depicted as results of a time-flow sequence initiated by primary basement disturbances which produce three major categories of surface response: secondary deformation, liquefaction and slope failure. Nine subcategories incorporate features produced by or resulting in: extruded sand, intruded sand, lateral spreading, faulting, subsidence of large areas, uplift of large areas, altered streams, coherent landslides, and incoherent landslides. The total morphoseismic features identified by this classification are 34 in number.« less

16. June 1974. BASEMENT, VIEW LOOKING SOUTHWEST, SHOWING THE MAIN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. June 1974. BASEMENT, VIEW LOOKING SOUTHWEST, SHOWING THE MAIN LINE SHAFT COMING THROUGH THE WALL FROM THE OTTO ENGINE. - Gruber Wagon Works, Pennsylvania Route 183 & State Hill Road at Red Bridge Park, Bernville, Berks County, PA

MTR BASEMENT. GENERAL ELECTRIC CONTROL CONSOLE FOR AIRCRAFT NUCLEAR PROPULSION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

MTR BASEMENT. GENERAL ELECTRIC CONTROL CONSOLE FOR AIRCRAFT NUCLEAR PROPULSION EXPERIMENT NO. 1. INL NEGATIVE NO. 6510. Unknown Photographer, 9/29/1959 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

11. INTERIOR DETAIL, BASEMENT, SHOWING CONDUITS LEADING UNDERGROUND TO SWITCHES ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. INTERIOR DETAIL, BASEMENT, SHOWING CONDUITS LEADING UNDERGROUND TO SWITCHES AND SIGNALS - Baltimore & Potomac Interlocking Tower, Adjacent to AMTRAK railroad tracks in block bounded by Howard Street, Jones Falls Expressway, Maryland Avenue & Falls Road, Baltimore, Independent City, MD

Stress rotation along pre-Cenozoic basement structures

NASA Astrophysics Data System (ADS)

Reiter, K.; Heidbach, O.; Henk, A.

2017-12-01

The in-situ stress state of the Earth's crust is under investigation since decades for both, scientific and economic purposes. Several methods have been established to indicate the contemporary orientation of the maximum compressive horizontal stress (SHmax). It is assumed that the same forces that drive plate motion are the first order stress sources and one could presume that SHmax is always parallel to plate motion, which is the case for some regions. However, deviations from this general trend occur in many regions. Therefore, second and third order sources of stress have been identified that potentially cause regional and local stress rotation with respect to the long wave-length trend imposed by plate tectonic forces. One group of such subordinate stress sources are lateral heterogeneities based on structures, petrothermal or petrophysical properties. The World Stress Map (WSM) project compiles systematically data records of the present day SHmax orientation. The increasing amount of stress orientation data allows to investigate areas with consistent stress rotation, divergent to the regional stress pattern. In our work we analyse the stress pattern variability and its causes beneath Germany. In the Molasse Basin in the Alpine foreland the SHmax orientation is perpendicular to the Alpine front as a consequence of gravitational potential energy of the orogen. SHmax is oriented in N-S direction in the central Alpine foreland and within the North German Basin. Between both, within the Mid-German Crystalline High, SHmax is divergent oriented in SE-NW direction. Neither gravitational potential energy nor petrothermal effects can be indicated as stress source. But when comparing the stress pattern with the Variscan basement structures it is obvious that SHmax is perpendicular oriented to this Palaeozoic basement structures. Therefore, petrophysical heterogeneities can be expected as reason for the observed stress rotation. Two assumptions can be made for the Mid

Regional differences in the expression of laminin isoforms during mouse neural tube development

PubMed Central

Copp, Andrew J.; Carvalho, Rita; Wallace, Adam; Sorokin, Lydia; Sasaki, Takako; Greene, Nicholas D.E.; Ybot-Gonzalez, Patricia

2013-01-01

Many significant human birth defects originate around the time of neural tube closure or early during post-closure nervous system development. For example, failure of the neural tube to close generates anencephaly and spina bifida, faulty cell cycle progression is implicated in primary microcephaly, while defective migration of neuroblasts can lead to neuronal migration disorders such as lissencephaly. At the stage of neural tube closure, basement membranes are becoming organised around the neuroepithelium, and beneath the adjacent non-neural surface ectoderm. While there is circumstantial evidence to implicate basement membrane dynamics in neural tube and surface ectodermal development, we have an incomplete understanding of the molecular composition of basement membranes at this stage. In the present study, we examined the developing basement membranes of the mouse embryo at mid-gestation (embryonic day 9.5), with particular reference to laminin composition. We performed in situ hybridization to detect the mRNAs of all eleven individual laminin chains, and immunohistochemistry to identify which laminin chains are present in the basement membranes. From this information, we inferred the likely laminin variants and their tissues of origin: that is, whether a given basement membrane laminin is contributed by epithelium, mesenchyme, or both. Our findings reveal major differences in basement composition along the body axis, with the rostral neural tube (at mandibular arch and heart levels) exhibiting many distinct laminin variants, while the lumbar level where the neural tube is just closing shows a much simpler laminin profile. Moreover, there appears to be a marked difference in the extent to which the mesenchyme contributes laminin variants to the basement membrane, with potential contribution of several laminins rostrally, but no contribution caudally. This information paves the way towards a mechanistic analysis of basement membrane laminin function during early

Modelling the role of basement block rotation and strike-slip faulting on structural pattern in the cover units of fold-and-thrust belts

NASA Astrophysics Data System (ADS)

Koyi, Hemin; Nilfouroushan, Faramarz; Hessami, Khaled

2015-04-01

A series of scaled analogue models are run to study the degree of coupling between basement block kinematics and cover deformation. In these models, rigid basal blocks were rotated about vertical axis in a "bookshelf" fashion, which caused strike-slip faulting along the blocks and, to some degrees, in the overlying cover units of loose sand. Three different combinations of cover basement deformations are modeled; cover shortening prior to basement fault movement; basement fault movement prior to shortening of cover units; and simultaneous cover shortening with basement fault movement. Model results show that the effect of basement strike-slip faults depends on the timing of their reactivation during the orogenic process. Pre- and syn-orogen basement strike-slip faults have a significant impact on the structural pattern of the cover units, whereas post-orogenic basement strike-slip faults have less influence on the thickened hinterland of the overlying fold-and-thrust belt. The interaction of basement faulting and cover shortening results in formation of rhomb features. In models with pre- and syn-orogen basement strike-slip faults, rhomb-shaped cover blocks develop as a result of shortening of the overlying cover during basement strike-slip faulting. These rhombic blocks, which have resemblance to flower structures, differ in kinematics, genesis and structural extent. They are bounded by strike-slip faults on two opposite sides and thrusts on the other two sides. In the models, rhomb-shaped cover blocks develop as a result of shortening of the overlying cover during basement strke-slip faulting. Such rhomb features are recognized in the Alborz and Zagros fold-and-thrust belts where cover units are shortened simultaneously with strike-slip faulting in the basement. Model results are also compared with geodetic results obtained from combination of all available GPS velocities in the Zagros and Alborz FTBs. Geodetic results indicate domains of clockwise and

Asymmetric Effects of Subaerial and Subaqueous Basement Slopes on Self-Similar Morphology of Prograding Deltas

NASA Astrophysics Data System (ADS)

Lai, Steven Yueh Jen; Hsiao, Yung-Tai; Wu, Fu-Chun

2017-12-01

Deltas form over basements of various slope configurations. While the morphodynamics of prograding deltas over single-slope basements have been studied previously, our understanding of delta progradation over segmented basements is still limited. Here we use experimental and analytical approaches to investigate the deltaic morphologies developing over two-slope basements with unequal subaerial and subaqueous slopes. For each case considered, the scaled profiles of the evolving delta collapse to a single profile for constant water and sediment influxes, allowing us to use the analytical self-similar profiles to investigate the individual effects of subaerial/subaqueous slopes. Individually varying the subaerial/subaqueous slopes exerts asymmetric effects on the morphologies. Increasing the subaerial slope advances the entire delta; increasing the subaqueous slope advances the upstream boundary of the topset yet causes the downstream boundary to retreat. The delta front exhibits a first-retreat-then-advance migrating trend with increasing subaqueous slope. A decrease in subaerial topset length is always accompanied by an increase in subaqueous volume fraction, no matter which segment is steepened. Applications are presented for estimating shoreline retreat caused by steepening of basement slopes, and estimating subaqueous volume and delta front using the observed topset length. The results may have implications for real-world delta systems subjected to upstream tectonic uplift and/or downstream subsidence. Both scenarios would exhibit reduced topset lengths, which are indicative of the accompanied increases in subaqueous volume and signal tectonic uplift and/or subsidence that are at play. We highlight herein the importance of geometric controls on partitioning of sediment between subaerial and subaqueous delta components.

A Thick, Deformed Sedimentary Wedge in an Erosional Subduction Zone, Southern Costa Rica

NASA Astrophysics Data System (ADS)

Silver, E. A.; Kluesner, J. W.; Edwards, J. H.; Vannucchi, P.

2014-12-01

A paradigm of erosional subduction zones is that the lower part of the wedge is composed of strong, crystalline basement (Clift and Vannucchi, Rev. Geophys., 42, RG2001, 2004). The CRISP 3D seismic reflection study of the southern part of the Costa Rica subduction zone shows quite the opposite. Here the slope is underlain by a series of fault-cored anticlines, with faults dipping both landward and seaward that root into the plate boundary. Deformation intensity increases with depth, and young, near-surface deformation follows that of the deeper structures but with basin inversions indicating a dynamic evolution (Edwards et al., this meeting). Fold wavelength increases landward, consistent with the folding of a landward-thickening wedge. Offscraping in accretion is minimal because incoming sediments on the lower plate are very thin. Within the wedge, thrust faulting dominates at depth in the wedge, whereas normal faulting dominates close to the surface, possibly reflecting uplift of the deforming anticlines. Normal faults form a mesh of NNW and ENE-trending structures, whereas thrust faults are oriented approximately parallel to the dominant fold orientation, which in turn follows the direction of roughness on the subducting plate. Rapid subduction erosion just prior to 2 Ma is inferred from IODP Expedition 334 (Vannucchi et al., 2013, Geology, 49:995-998). Crystalline basement may have been largely removed from the slope region during this rapid erosional event, and the modern wedge may consist of rapidly redeposited material (Expedition 344 Scientists, 2013) that has been undergoing deformation since its inception, producing a structure quite different from that expected of an eroding subduction zone.

Basement hall under the northeast part of the building. Live ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Basement hall under the northeast part of the building. Live animal cages and dissection rooms are to the right. Note concrete footings. - San Bernardino Valley College, Life Science Building, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

Fault-zone structure and weakening processes in basin-scale reverse faults: The Moonlight Fault Zone, South Island, New Zealand

NASA Astrophysics Data System (ADS)

Alder, S.; Smith, S. A. F.; Scott, J. M.

2016-10-01

The >200 km long Moonlight Fault Zone (MFZ) in southern New Zealand was an Oligocene basin-bounding normal fault zone that reactivated in the Miocene as a high-angle reverse fault (present dip angle 65°-75°). Regional exhumation in the last c. 5 Ma has resulted in deep exposures of the MFZ that present an opportunity to study the structure and deformation processes that were active in a basin-scale reverse fault at basement depths. Syn-rift sediments are preserved only as thin fault-bound slivers. The hanging wall and footwall of the MFZ are mainly greenschist facies quartzofeldspathic schists that have a steeply-dipping (55°-75°) foliation subparallel to the main fault trace. In more fissile lithologies (e.g. greyschists), hanging-wall deformation occurred by the development of foliation-parallel breccia layers up to a few centimetres thick. Greyschists in the footwall deformed mainly by folding and formation of tabular, foliation-parallel breccias up to 1 m wide. Where the hanging-wall contains more competent lithologies (e.g. greenschist facies metabasite) it is laced with networks of pseudotachylyte that formed parallel to the host rock foliation in a damage zone extending up to 500 m from the main fault trace. The fault core contains an up to 20 m thick sequence of breccias, cataclasites and foliated cataclasites preserving evidence for the progressive development of interconnected networks of (partly authigenic) chlorite and muscovite. Deformation in the fault core occurred by cataclasis of quartz and albite, frictional sliding of chlorite and muscovite grains, and dissolution-precipitation. Combined with published friction and permeability data, our observations suggest that: 1) host rock lithology and anisotropy were the primary controls on the structure of the MFZ at basement depths and 2) high-angle reverse slip was facilitated by the low frictional strength of fault core materials. Restriction of pseudotachylyte networks to the hanging-wall of the

An Interactive GIS Procedure for Building and Basement Corrections in Urban Microgravity Surveys

NASA Astrophysics Data System (ADS)

Chasseriau, P.; Olivier, R.

2007-12-01

Construction of a new underground railway in Lausanne, a highly-urbanized city in Switzerland, was an opportunity to test the feasibility and reliability of microgravity surveys in urban environments. The goal of our microgravity survey was to determine the depth-to-bedrock along the project corridor. Available drilling information allowed us verify the density model obtained. The geophysical results also provided spatially exhaustive subsurface information that could not be obtained with drilling methods alone. Gravimetry is one of the rare geophysical methods that can be used in noisy urban environments. An inevitable constraint of this method is terrain correction. It is not easy to obtain a simple and accurate digital elevation model (DEM) of an urban environment considering that buildings and basements are not included. However, these structures significantly influence gravity measurements. We calculate, with software that we have developed, the influence of buildings and basements in order to correct our gravity data. Our procedure permits the integration of gravity measurements, cadastral information (building typology and geometry) and basement geometry in an Access database that allows interactive determination of the Bouguer anomaly. A geographic information system (GIS) is used to extract building geometries based on cadastral information and to correct the influence of each building using a simplified architectural style. Basement voids are then introduced in the final DEM using building outlines given by cadastral maps. The depths and altitudes of the basements are measured by visiting them, and then linking the results to a regional topographic map. All of these corrections can be calculated before the gravity acquisition has begun in order to optimize the design of the survey. The surveys are executed late at night so as to minimize the effects of traffic noise. 160 gravity measurements were carried out before and after digging of the underground

57. Entry door (open), BE16, basement level, building 500, looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

57. Entry door (open), BE-16, basement level, building 500, looking southeast - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

54. Room BF14, IDA room, basement level, building 500, looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

54. Room BF-14, IDA room, basement level, building 500, looking west - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

56. Entry door (closed), BB16, basement level, building 500, looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

56. Entry door (closed), BB-16, basement level, building 500, looking southeast - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

2D Seismic Velocity Modelling in the Southeastern Romanian Carpathians and its Foreland (Vrancea Zone and Focsani Basin)

NASA Astrophysics Data System (ADS)

Stephenson, R.; Bocin, A.; Tryggvason, A.

2003-12-01

The DACIA-PLAN (Danube and Carpathian Integrated Action on Processes in the Lithosphere and Neotectonics) deep seismic reflection survey was performed in August-September 2001, with the objective of obtaining of new information on the deep structure of the external Carpathians nappes and the architecture of Tertiary/Quaternary basins developed within and adjacent to the seismically-active Vrancea Zone, including the rapidly subsiding Focsani Basin. The DACIA-PLAN profile is about 140 km long, having a roughly NW-SE direction, from near the southeast Transylvanian Basin, across the mountainous southeastern Carpathians and their foreland to near the Danube Dalta. A high resolution 2D velocity model of the upper crust along the seismic profile has been determined from a first-arrival tomographic inversion of the DACIA-PLAN data. The shallowing of Palaeozoic-Mesozoic basement, and related structural heterogeneity within it, beneath the eastern flank of the Focsani Basin is clearly seen. Velocity heterogeneity within the Carpathian nappe belt is also evident and is indicative of internal structural complexity, including the presence of salt bodies and basement involvement in thrusting, thus favouring some current geological models over others. The presence of basement involvement implies the compressional reactivation of pre-existing basement normal faults. Members of the DACIA-PLAN/TomoSeis Working Group (see poster) should be considered as co-authors of this presentation.

The Vardar zone as a suture for the Mirdita ophiolites, Albania: Constraints from the structural analysis of the Korabi-Pelagonia zone

NASA Astrophysics Data System (ADS)

Tremblay, Alain; Meshi, Avni; Deschamps, Thomas; Goulet, François; Goulet, Normand

2015-02-01

The Dinarides-Hellenides result from underthrusting of the Adriatic margin during Africa-Europe convergence. In Albania, they consist of (1) a western zone of nappes derived from Adria; (2) a central belt made up of the Mirdita ophiolites; and (3) an eastern zone, the Korabi-Pelagonia zone, of Variscan basement overlain by Permian to Mesozoic rift deposits and carbonates. Some authors interpret the Korabi-Pelagonia zone as a microcontinent between the Mirdita-Pindos oceanic basin to the west and the eastern Vardar oceanic basin to the east; other regard the Korabi-Pelagonia zone as a tectonic window below a single ophiolitic nappe. This contribution argues for a far-traveled thrust sheet. The Mirdita ophiolites are 165-160 Ma. The metamorphic sole yielded 40Ar/39Ar ages of 171 to 162 Ma. The Korabi-Pelagonia zone is subdivided into the Korabi and Gjegjan subzones. The structural analysis of these rocks supports the rooting of the Mirdita ophiolites in the Western Vardar zone. The post-Variscan cover sequence of the Korabi subzone records two phases of deformation: D1 is associated with a SE dipping to flat-lying schistosity axial planar to NW verging folds and thrust faults, related to ophiolite obduction; D2 is a postobduction NNE trending crenulation cleavage. Published zircon fission track analyses yielded 150-125 Ma, suggesting that regional metamorphism is Early Cretaceous or older. K-Ar mica ages from correlative rocks of Macedonia cluster between 148 and 130 Ma, indicating that D1 is Late Jurassic. A west directed obduction is favored, as is a rooting east of the Mirdita ophiolites because of the top-to-the-west structural polarity of obduction-related deformation.

Numerical models of pore pressure and stress changes along basement faults due to wastewater injection: Applications to the 2014 Milan, Kansas Earthquake

USGS Publications Warehouse

Hearn, Elizabeth H.; Koltermann, Christine; Rubinstein, Justin R.

2018-01-01

We have developed groundwater flow models to explore the possible relationship between wastewater injection and the 12 November 2014 Mw 4.8 Milan, Kansas earthquake. We calculate pore pressure increases in the uppermost crust using a suite of models in which hydraulic properties of the Arbuckle Formation and the Milan earthquake fault zone, the Milan earthquake hypocenter depth, and fault zone geometry are varied. Given pre‐earthquake injection volumes and reasonable hydrogeologic properties, significantly increasing pore pressure at the Milan hypocenter requires that most flow occur through a conductive channel (i.e., the lower Arbuckle and the fault zone) rather than a conductive 3‐D volume. For a range of reasonable lower Arbuckle and fault zone hydraulic parameters, the modeled pore pressure increase at the Milan hypocenter exceeds a minimum triggering threshold of 0.01 MPa at the time of the earthquake. Critical factors include injection into the base of the Arbuckle Formation and proximity of the injection point to a narrow fault damage zone or conductive fracture in the pre‐Cambrian basement with a hydraulic diffusivity of about 3–30 m2/s. The maximum pore pressure increase we obtain at the Milan hypocenter before the earthquake is 0.06 MPa. This suggests that the Milan earthquake occurred on a fault segment that was critically stressed prior to significant wastewater injection in the area. Given continued wastewater injection into the upper Arbuckle in the Milan region, assessment of the middle Arbuckle as a hydraulic barrier remains an important research priority.

55. Room BF9, paper shredding facility, basement level, building 500, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

55. Room BF-9, paper shredding facility, basement level, building 500, looking east - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Headquarters Building, 901 SAC Boulevard, Bellevue, Sarpy County, NE

PBF (PER620) interior, basement level. Sampling equipment. Date: May 2004. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF (PER-620) interior, basement level. Sampling equipment. Date: May 2004. INEEL negative no. HD-41-5-4 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

Origin of a crustal splay fault and its relation to the seismogenic zone and underplating at the erosional north Ecuador-south Colombia oceanic margin

NASA Astrophysics Data System (ADS)

Collot, J.-Y.; Agudelo, W.; Ribodetti, A.; Marcaillou, B.

2008-12-01

Splay faults within accretionary complexes are commonly associated with the updip limit of the seismogenic zone. Prestack depth migration of a multichannel seismic line across the north Ecuador-south Colombia oceanic margin images a crustal splay fault that correlates with the seaward limit of the rupture zone of the 1958 (Mw 7.7) tsunamogenic subduction earthquake. The splay fault separates 5-6.6 km/s velocity, inner wedge basement rocks, which belong to the accreted Gorgona oceanic terrane, from 4 to 5 km/s velocity outer wedge rocks. The outer wedge is dominated by basal tectonic erosion. Despite a 3-km-thick trench fill, subduction of 2-km-high seamount prevented tectonic accretion and promotes basal tectonic erosion. The low-velocity and poorly reflective subduction channel that underlies the outer wedge is associated with the aseismic, décollement thrust. Subduction channel fluids are expected to migrate upward along splay faults and alter outer wedge rocks. Conversely, duplexes are interpreted to form from and above subducting sediment, at ˜14- to 15-km depths between the overlapping seismogenic part of the splay fault and the underlying aseismic décollement. Coeval basal erosion of the outer wedge and underplating beneath the apex of inner wedge control the margin mass budget, which comes out negative. Intraoceanic basement fossil listric normal faults and a rift zone inverted in a flower structure reflect the evolution of the Gorgona terrane from Cretaceous extension to likely Eocene oblique compression. The splay faults could have resulted from tectonic inversion of listric normal faults, thus showing how inherited structures may promote fluid flow across margin basement and control seismogenesis.

Matrix Metalloproteinase Dysregulation in the Stria Vascularis of Mice with Alport Syndrome

PubMed Central

Gratton, Michael Anne; Rao, Velidi H.; Meehan, Daniel T.; Askew, Charles; Cosgrove, Dominic

2005-01-01

Alport syndrome results from mutations in genes encoding collagen α3(IV), α4(IV), or α5(IV) and is characterized by progressive glomerular disease associated with a high-frequency sensorineural hearing loss. Earlier studies of a gene knockout mouse model for Alport syndrome noted thickening of strial capillary basement membranes in the cochlea, suggesting that the stria vascularis is the primary site of cochlear pathogenesis. Here we combine a novel cochlear microdissection technique with molecular analyses to illustrate significant quantitative alterations in strial expression of mRNAs encoding matrix metalloproteinases-2, -9, -12, and -14. Gelatin zymography of extracts from the stria vascularis confirmed these findings. Treatment of Alport mice with a small molecule inhibitor of these matrix metalloproteinases exacerbated strial capillary basement membrane thickening, demonstrating that alterations in basement membrane metabolism result in matrix accumulation in the strial capillary basement membranes. This is the first demonstration of true quantitative analysis of specific mRNAs for matrix metalloproteinases in a cochlear microcompartment. Further, these data suggest that the altered basement membrane composition in Alport stria influences the expression of genes involved in basement membrane metabolism. PMID:15855646

7. AGENT STORAGE TANKS LOCATED IN CONCRETE BASEMENT. PHOTOGRAPH IS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. AGENT STORAGE TANKS LOCATED IN CONCRETE BASEMENT. PHOTOGRAPH IS OF THE EASTERN MOST TANK LOOKING SOUTH. - Rocky Mountain Arsenal, Tank House, Quadrant 1, approximately 1000 feet South of December Seventh Avenue; 2200 feet East of D Street, Commerce City, Adams County, CO

22. INTERIOR VIEW, BASEMENT UNDER NORTH ROOM OF MAIN BLOCK, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. INTERIOR VIEW, BASEMENT UNDER NORTH ROOM OF MAIN BLOCK, VIEW OF NORTHWEST WALL SHOWING CORBELING BASE OF FIRST FLOOR CHIMNEY BLOCK WITH STOVE-PIPE HOLE, AND MORTISE AND TENON FRAMING FOR HEARTH BED - Clifton Farm, Off Baker Road, Frederick, Frederick County, MD

17. INTERIOR VIEW, BASEMENT, LOOKING SOUTHWEST AT THE GEAR PIT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

17. INTERIOR VIEW, BASEMENT, LOOKING SOUTHWEST AT THE GEAR PIT BELOW THE GRINDING STONES, SHOWING WOODEN COGS ATTACHED TO UNDERGROUND TURBINES. FRICTION DRIVE VISIBLE BEHIND CONTROL BAR (LEFT) WHICH OPERATES SMUT MILL - Schech's Mill, Beaver Creek State Park, La Crescent, Houston County, MN

71. (Credit JTL) Pipe gallery looking south in basement underneath ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

71. (Credit JTL) Pipe gallery looking south in basement underneath 1910-11 and 1924 filter wing extensions. Note bottoms of converted New York horizontal pressure filters in right background. - McNeil Street Pumping Station, McNeil Street & Cross Bayou, Shreveport, Caddo Parish, LA

6. VIEW OF BASEMENT, LOOKING NORTHEAST TOWARD THIRD TURBINE. VISIBLE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. VIEW OF BASEMENT, LOOKING NORTHEAST TOWARD THIRD TURBINE. VISIBLE IS THE LINE SHAFTING THAT DROVE THE MILL'S WATER-POWERED MACHINERY. Photographer: Jet T. Lowe, 1985 - Alexander's Grist Mill, Lock 37 on Ohio & Erie Canal, South of Cleveland, Valley View, Cuyahoga County, OH

Neotectonic reactivation of shear zones and implications for faulting style and geometry in the continental margin of NE Brazil

NASA Astrophysics Data System (ADS)

Bezerra, F. H. R.; Rossetti, D. F.; Oliveira, R. G.; Medeiros, W. E.; Neves, B. B. Brito; Balsamo, F.; Nogueira, F. C. C.; Dantas, E. L.; Andrades Filho, C.; Góes, A. M.

2014-02-01

The eastern continental margin of South America comprises a series of rift basins developed during the breakup of Pangea in the Jurassic-Cretaceous. We integrated high resolution aeromagnetic, structural and stratigraphic data in order to evaluate the role of reactivation of ductile, Neoproterozoic shear zones in the deposition and deformation of post-rift sedimentary deposits in one of these basins, the Paraíba Basin in northeastern Brazil. This basin corresponds to the last part of the South American continent to be separated from Africa during the Pangea breakup. Sediment deposition in this basin occurred in the Albian-Maastrichtian, Eocene-Miocene, and in the late Quaternary. However, our investigation concentrates on the Miocene-Quaternary, which we consider the neotectonic period because it encompasses the last stress field. This consisted of an E-W-oriented compression and a N-S-oriented extension. The basement of the basin forms a slightly seaward-tilted ramp capped by a late Cretaceous to Quaternary sedimentary cover ~ 100-400 m thick. Aeromagnetic lineaments mark the major steeply-dipping, ductile E-W- to NE-striking shear zones in this basement. The ductile shear zones mainly reactivated as strike-slip, normal and oblique-slip faults, resulting in a series of Miocene-Quaternary depocenters controlled by NE-, E-W-, and a few NW-striking faults. Faulting produced subsidence and uplift that are largely responsible for the present-day morphology of the valleys and tablelands in this margin. We conclude that Precambrian shear zone reactivation controlled geometry and orientation, as well as deformation of sedimentary deposits, until the Neogene-Quaternary.

Equipment Location Plan, partial basement plan. (Includes identification of each ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Equipment Location Plan, partial basement plan. (Includes identification of each separate CPU, tape drive, hard drive, printer, keyboard, etc., within the data processing center in the southeast part of the basement.) March Air Force Base, Riverside, California, Combat Operations Center, 465-L DPC. By International Electric Corporation, Paramus, New Jersey (3/5/62); for Moffatt and Nichol, Engineers, 122 West Fifth Street, Long Beach, California; for the Corps of Engineers, U.S. Army, Office of the District Engineer, Los Angeles, California. Drawing no. AW-60-02-03, sheet no. 100, approved March, 1962; specifications no. OCI-62-66; D.O. series AW 1596/100, Rev. "A"; file drawer 1290. Last revised 3 October 1966. Scale one-quarter inch to one foot. 28.75x40.5 inches. ink on linen - March Air Force Base, Strategic Air Command, Combat Operations Center, 5220 Riverside Drive, Moreno Valley, Riverside County, CA

PBF (PER620) interior, first basement level. Sampling equipment. Date: March ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF (PER-620) interior, first basement level. Sampling equipment. Date: March 2004. INEEL negative no. HD-41-4-1 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

Basement thrust sheets in the Clearwater orogenic zone, central Idaho and western Montana ( USA).

USGS Publications Warehouse

Skipp, B.

1987-01-01

The Clearwater orogenic zone in central Idaho and W Montana contains at least 2 major NE-directed Cordilleran thrust plates of Early Proterozoic metasedimentary and metaigneous rocks that overrode previously folded Middle Proterozoic rocks of the Belt basin in Cretaceous time. The northeastward migration of the resultant thickened wedge of crustal material combined with Cretaceous subduction along the W continental margin produced a younger N Bitterroot lobe of the Idaho batholith relative to an older S Atlanta lobe. Eocene extensional unroofing and erosion of the Bitterroot lobe has exposed the roots of the thick Cordilleran thrust sheets.-Author

The Proterozoic Mount Isa Fault Zone, northeastern Australia: is it really a ca. 1.9 Ga terrane-bounding suture?

NASA Astrophysics Data System (ADS)

Bierlein, Frank P.; Betts, Peter G.

2004-09-01

In marked contrast to Palaeoproterozoic Laurentia, the location of sutures and boundaries of discrete crustal fragments amalgamated during Palaeoproterozoic formation of the North Australian Craton remain highly speculative. Interpretations of suture locations have relied heavily on the analysis of regional geophysical datasets because of sparse exposure of rocks of the appropriate age. The Mount Isa Fault Zone has been interpreted as one such Palaeoproterozoic terrane-bounding suture. Furthermore, the coincidence of this fault zone with major shale-hosted massive sulphide Pb-Zn-Ag orebodies has led to speculations that trans-lithospheric faults may be an important ingredient for the development of this deposit type. This study has integrated geophysical and geochemical data to test the statute of the Mount Isa Fault as a terrane-bounding suture. Forward modelling of gravity data shows that basement rocks on either side of the Mount Isa Fault have similar densities. These interpretations are consistent with geochemical observations and Sm-Nd data that suggest that basement lithologies on either side of the Mount Isa Fault are geochemically and isotopically indistinguishable from each other, and that the Mount Isa Fault is unlikely to represent a suture zone that separates different Palaeoproterozoic terranes. Our data indicate that the crustal blocks on both sides of the Mount Isa Fault Zone must have been in within close proximity of each other since the Palaeoproterozoic, and that the Western Fold Belt was part of the (ancestral) North Australian Craton well before the ˜1.89-1.87 Ga Barramundi Orogeny. It appears that deep crustal variations in density may be related to the boundary between a shallowly west-dipping high-density mafic to ultramafic plate and low-density basement rocks. This interpretation in turn impacts on crustal-scale models for the development of shale-hosted massive sulphide Pb-Zn mineralisation, which do not require trans

PBF (PER620) interior, basement level. Detail of coolant piping. Date: ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF (PER-620) interior, basement level. Detail of coolant piping. Date: May 2004. INEEL negative no. HD-41-5-2 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

Membrane Assembly during the Infection Cycle of the Giant Mimivirus

PubMed Central

Mutsafi, Yael; Shimoni, Eyal; Shimon, Amir; Minsky, Abraham

2013-01-01

Although extensively studied, the structure, cellular origin and assembly mechanism of internal membranes during viral infection remain unclear. By combining diverse imaging techniques, including the novel Scanning-Transmission Electron Microscopy tomography, we elucidate the structural stages of membrane biogenesis during the assembly of the giant DNA virus Mimivirus. We show that this elaborate multistage process occurs at a well-defined zone localized at the periphery of large viral factories that are generated in the host cytoplasm. Membrane biogenesis is initiated by fusion of multiple vesicles, ∼70 nm in diameter, that apparently derive from the host ER network and enable continuous supply of lipid components to the membrane-assembly zone. The resulting multivesicular bodies subsequently rupture to form large open single-layered membrane sheets from which viral membranes are generated. Membrane generation is accompanied by the assembly of icosahedral viral capsids in a process involving the hypothetical major capsid protein L425 that acts as a scaffolding protein. The assembly model proposed here reveals how multiple Mimivirus progeny can be continuously and efficiently generated and underscores the similarity between the infection cycles of Mimivirus and Vaccinia virus. Moreover, the membrane biogenesis process indicated by our findings provides new insights into the pathways that might mediate assembly of internal viral membranes in general. PMID:23737745

113. ENTRANCE TO GOLD AREA SECURITY ROOM IN BASEMENT, LOCATED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

113. ENTRANCE TO GOLD AREA SECURITY ROOM IN BASEMENT, LOCATED IN BOTTOM OF ORIGINAL WET-BUCKET ELEVATOR SHAFT, ADJACENT TO DIESTER TABLE ROOM. NOTE BOARD WITH INDIVIDUAL TAGS FOR GOLD AREA EMPLOYEES. - Shenandoah-Dives Mill, 135 County Road 2, Silverton, San Juan County, CO

Pneumatic vacuum tube message center, basement room 23, looking southeast ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Pneumatic vacuum tube message center, basement room 23, looking southeast toward doorway and corridor. Note soundproof walls, pedestal flooring, and cable tray suspended from the ceiling - March Air Force Base, Strategic Air Command, Combat Operations Center, 5220 Riverside Drive, Moreno Valley, Riverside County, CA

10. Historic American Buildings Survey Photocopy BASEMENT PLAN, NORTH ELEVATION, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Historic American Buildings Survey Photocopy BASEMENT PLAN, NORTH ELEVATION, ARCHITECT'S ORIGINAL PLAN Restricted: Not to be reproduced without written permission from Beinecke Rare Books Library, Yale University, New Haven, Conn. - John Pitkin Norton House, 52 Hillhouse Avenue, New Haven, New Haven County, CT

Prostate-specific membrane antigen PET/MRI validation of MR textural analysis for detection of transition zone prostate cancer.

PubMed

Bates, Anthony; Miles, Kenneth

2017-12-01

To validate MR textural analysis (MRTA) for detection of transition zone (TZ) prostate cancer through comparison with co-registered prostate-specific membrane antigen (PSMA) PET-MR. Retrospective analysis was performed for 30 men who underwent simultaneous PSMA PET-MR imaging for staging of prostate cancer. Thirty texture features were derived from each manually contoured T2-weighted, transaxial, prostatic TZ using texture analysis software that applies a spatial band-pass filter and quantifies texture through histogram analysis. Texture features of the TZ were compared to PSMA expression on the corresponding PET images. The Benjamini-Hochberg correction controlled the false discovery rate at <5%. Eighty-eight T2-weighted images in 18 patients demonstrated abnormal PSMA expression within the TZ on PET-MR. 123 images were PSMA negative. Based on the corrected p-value of 0.005, significant differences between PSMA positive and negative slices were found for 16 texture parameters: Standard deviation and mean of positive pixels for all spatial filters (p = <0.0001 for both at all spatial scaling factor (SSF) values) and mean intensity following filtration for SSF 3-6 mm (p = 0.0002-0.0018). Abnormal expression of PSMA within the TZ is associated with altered texture on T2-weighted MR, providing validation of MRTA for the detection of TZ prostate cancer. • Prostate transition zone (TZ) MR texture analysis may assist in prostate cancer detection. • Abnormal transition zone PSMA expression correlates with altered texture on T2-weighted MR. • TZ with abnormal PSMA expression demonstrates significantly reduced MI, SD and MPP.

Rock strength measurements on Archaean basement granitoids recovered from scientific drilling in the active Koyna seismogenic zone, western India

NASA Astrophysics Data System (ADS)

Goswami, Deepjyoti; Akkiraju, Vyasulu V.; Misra, Surajit; Roy, Sukanta; Singh, Santosh K.; Sinha, Amalendu; Gupta, Harsh; Bansal, B. K.; Nayak, Shailesh

2017-08-01

Reservoir triggered earthquakes have been occurring in the Koyna area, western India for the past five decades. Triaxial tests carried out on 181 core samples of Archaean granitoids underlying the Deccan Traps provide valuable constraints on rock strength properties in the Koyna seismogenic zone for the first time. The data include measurements on granite gneiss, granite, migmatitic gneiss and mylonitised granite gneiss obtained from boreholes KBH-3, KBH-4A, KBH-5 and KBH-7 located in the western and eastern margins of the seismic zone. Salient results are as follows. (i) Increase of rock strength with increasing confining pressure allow determination of the linearized failure envelopes from which the cohesive strength and angle of internal friction are calculated. (ii) Variable differential stresses at different depths are the manifestations of deformation partitioning in close association of fault zone(s) or localized fracture zones. (iii) Fractures controlled by naturally developed weak planes such as cleavage and fabric directly affect the rock strength properties, but the majority of failure planes developed during triaxial tests is not consistent with the orientations of pre-existing weak planes. The failure planes may, therefore, represent other planes of weakness induced by ongoing seismic activity. (iv) Stress-strain curves confirm that axial deformation is controlled by the varying intensity of pre-existing shear in the granitoids, viz., mylonite, granite gneiss and migmatitic gneiss. (v) Frequent occurrences of low magnitude earthquakes may be attributed to low and variable rock strength of the granitoids, which, in turn, is modified by successive seismic events.

Pyrenean hyper-extension : breaking, thinning, or stretching of the crust ? A view from the central north-Pyrenean zone

NASA Astrophysics Data System (ADS)

de Saint Blanquat, Michel; Bajolet, Flora; Boulvais, Philippe; Boutin, Alexandre; Clerc, Camille; Delacour, Adélie; Deschamp, Fabien; Ford, Mary; Fourcade, Serge; Gouache, Corentin; Grool, Arjan; Labaume, Pierre; Lagabrielle, Yves; Lahfid, Abdeltif; Lemirre, Baptiste; Monié, Patrick; de Parseval, Philippe; Poujol, Marc

2017-04-01

The geology of the North Pyrenean Zone in the central Pyrenees allows for the observation in the field of the entire section of the Pyrenean rift, from the mantle to the crust and the Mesozoic cover (pre, syn and post rift). The good knowledge we have of the pre-Alpine history of the Pyrenees allows us to properly constrain the Alpine geological evolution of the pre-Triassic rocks which record both Variscan and Alpine orogenic cycles. The mantle outcrop as kilometric to centimetric fragments of peridotite dispersed within a carbonate metamorphic breccia. The study of peridotite serpentinisation shows several events of low-temperature serpentinisation, in contact with seawater. In some locallities, we can observe a mixture of fragments of variously serpentinized peridotites. This suggests a tectonic context where fragments of peridotites from different structural levels were sampled more or less synchronously. The granulitic basement is characterized by a Variscan syndeformational HT event (300-280 Ma). So far we have not found any trace of a Cretaceous HT event (> 500°C). On the other hand, the basement is affected by a regional metasomatism that began during the Jurassic and became more spatially focused with time until it was restricted to the Pyrenean rift during the Aptien, Albian and Cenomanian. The talc-chlorite metasomatism (120-95 Ma) shows an evolution from a static toward a syn-deformation hydrothermal event, under a more or less normal geothermal gradient. Extensional deformation is recorded by the reworking of several inherited low-angle Variscan tectonic contacts, but also by dispersed high-angle extensional shear zones formed under greenshist conditions. The metamorphic Mesozoic cover of the basement massifs, which constitute the so-called Internal Metamorphic Zone, is an allochtonous unit made of lenses of Mesozoic rocks enclosed into the breccia, which locally contains peridotite and basement clasts. The Mesozoic metamorphic carbonates show a first

30. VIEW WEST IN BASEMENT OF BUILDING 41A; NOTE PLASTIC ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

30. VIEW WEST IN BASEMENT OF BUILDING 41A; NOTE PLASTIC DUCTING WHICH WAS USED TO VENT CORROSIVE FUMES FROM ACID PICKLING AND ELECTROPLATING TANKS - Scovill Brass Works, 59 Mill Street, Waterbury, New Haven County, CT

29. Basement under central corridor. Shaft on right actuates cross ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. Basement under central corridor. Shaft on right actuates cross over valve. Shaft at left operates main flood valve to admit water into the bed. - Lake Whitney Water Filtration Plant, Filtration Plant, South side of Armory Street between Edgehill Road & Whitney Avenue, Hamden, New Haven County, CT

NORTH BASEMENT WALL. IBEAM COLUMNS HAVE BEEN ENCASED IN CONCRETE. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

NORTH BASEMENT WALL. I-BEAM COLUMNS HAVE BEEN ENCASED IN CONCRETE. STEEL BEAMS LAY ACROSS FIRST FLOOR AWAITING CONCRETE POUR. CAMERA LOOKS SOUTHWEST. INL NEGATIVE NO. 735. Unknown Photographer, 10/6/1950 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

VIEW OF PROCESS DEVELOPMENT PILE (PDP) TANK, LOOKING WESTSOUTHWEST, BASEMENT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF PROCESS DEVELOPMENT PILE (PDP) TANK, LOOKING WEST-SOUTHWEST, BASEMENT LEVEL -15’. EDGE O FRESONANCE TEST REACTOR (RTR), LATER KNOWN AS LATTICE TEST REACTOR (LTR), VISIBLE TO RIGHT OF PDP TANK - Physics Assembly Laboratory, Area A/M, Savannah River Site, Aiken, Aiken County, SC

The mafic-ultramafic complex of Aniyapuram, Cauvery Suture Zone, southern India: Petrological and geochemical constraints for Neoarchean suprasubduction zone tectonics

NASA Astrophysics Data System (ADS)

Yellappa, T.; Venkatasivappa, V.; Koizumi, T.; Chetty, T. R. K.; Santosh, M.; Tsunogae, T.

2014-12-01

Several Precambrian mafic-ultramafic complexes occur along the Cauvery Suture Zone (CSZ) in Southern Granulite Terrain, India. Their origin, magmatic evolution and relationship with the associated high-grade rocks have not been resolved. The Aniyapuram Mafic-Ultramafic Complex (AMUC), the focus of the present study in southern part of the CSZ, is dominantly composed of peridotites, pyroxenites, gabbros, metagabbros/mafic granulites, hornblendites, amphibolites, plagiogranites, felsic granulites and ferruginous cherts. The rock types in the AMUC are structurally emplaced within hornblende gneiss (TTG) basement rocks and are highly deformed. The geochemical signature of the amphibolites indicates tholeiitic affinity for the protolith with magma generation in island arc-setting. N-MORB normalized pattern of the amphibolites show depletion in HFS-elements (P, Zr, Sm, Ti, and Y) and enrichment of LIL-elements (Rb, Ba, Th, Sr) with negative Nb anomalies suggesting involvement of subduction component in the depleted mantle source and formation in a supra-subduction zone tectonic setting. Our new results when correlated with the available age data suggest that the lithological association of AMUC represent the remnants of the Neoarchean oceanic lithosphere.

Dynamical Organization of Syntaxin-1A at the Presynaptic Active Zone

PubMed Central

Ullrich, Alexander; Böhme, Mathias A.; Schöneberg, Johannes; Depner, Harald; Sigrist, Stephan J.; Noé, Frank

2015-01-01

Synaptic vesicle fusion is mediated by SNARE proteins forming in between synaptic vesicle (v-SNARE) and plasma membrane (t-SNARE), one of which is Syntaxin-1A. Although exocytosis mainly occurs at active zones, Syntaxin-1A appears to cover the entire neuronal membrane. By using STED super-resolution light microscopy and image analysis of Drosophila neuro-muscular junctions, we show that Syntaxin-1A clusters are more abundant and have an increased size at active zones. A computational particle-based model of syntaxin cluster formation and dynamics is developed. The model is parametrized to reproduce Syntaxin cluster-size distributions found by STED analysis, and successfully reproduces existing FRAP results. The model shows that the neuronal membrane is adjusted in a way to strike a balance between having most syntaxins stored in large clusters, while still keeping a mobile fraction of syntaxins free or in small clusters that can efficiently search the membrane or be traded between clusters. This balance is subtle and can be shifted toward almost no clustering and almost complete clustering by modifying the syntaxin interaction energy on the order of only 1 kBT. This capability appears to be exploited at active zones. The larger active-zone syntaxin clusters are more stable and provide regions of high docking and fusion capability, whereas the smaller clusters outside may serve as flexible reserve pool or sites of spontaneous ectopic release. PMID:26367029

Membranous glomerulopathy with spherules: an uncommon variant with obscure pathogenesis.

PubMed

Kowalewska, Jolanta; Smith, Kelly D; Hudkins, Kelly L; Chang, Anthony; Fogo, Agnes B; Houghton, Donald; Leslie, Deena; Aitchison, John; Nicosia, Roberto F; Alpers, Charles E

2006-06-01

Occasional case reports of membranous glomerulopathy described unique subepithelial accumulations of an unusual type of immune deposit composed of spherular structures. The identity of such structures as nuclear pores has been suggested, but not established. We identified a cohort of patients (n = 14, including 1 patient with disease recurrence in an allograft) who presented with nephrotic syndrome and had renal biopsy specimens with light and immunofluorescence microscopic findings characteristic of membranous glomerulopathy. These patients were distinguished by ultrastructural studies that showed glomerular capillary wall accumulations of subepithelial immune deposits composed of uniform spherular structures, while lacking the typical granular electron-dense deposits seen in membranous glomerulopathy. The molecular identity of these spherular structures as nuclear pores was tested by using immunofluorescence microscopy and immunohistochemistry with mouse monoclonal antinuclear pore antibodies (Covance, Princeton, NJ) and anti-Nuclear Pore-O-Linked Glycoprotein (Affinity BioReagents Inc, Golden, CO) antibodies. Measurement of spherular structures by using high-magnification electron microscopy showed an average diameter of 84.5 nm, which correlated well with accepted diameters of nuclear pores (80 to 120 nm). Immunofluorescence microscopy and immunoperoxidase staining with both antibodies showed characteristic beaded staining of nuclear membranes of multiple cell types within normal control kidney, but no staining of immune-type deposits within glomerular basement membranes. These cases form a rare, but distinctive, morphological subclass of membranous glomerulopathy. The antigenic specificity of immune deposits in these cases remains elusive.

Pathophysiology of Corneal Scarring in Persistent Epithelial Defects After PRK and Other Corneal Injuries.

PubMed

Wilson, Steven E; Medeiros, Carla S; Santhiago, Marcony R

2018-01-01

To analyze corneal persistent epithelial defects that occurred at 3 to 4 weeks after -4.50 diopter (D) photorefractive keratectomy (PRK) in rabbits and apply this pathophysiology to the treatment of persistent epithelial defects that occur after any corneal manipulations or diseases. Two of 168 corneas that had -4.50 D PRK to study epithelial basement membrane regeneration developed spontaneous persistent epithelial defects that did not heal at 3 weeks after PRK. These were studied with slit-lamp photographs, immunohistochemistry for the myofibroblast marker alpha-smooth muscle actin (α-SMA), and transmission electron microscopy. Myofibroblasts developed at the stromal surface within the persistent epithelial defect and for a short distance peripheral to the leading edge of the epithelium. No normal epithelial basement membrane was detectable within the persistent epithelial defect or for up to 0.3 mm behind the leading edge of the epithelium, although epithelial basement membrane had normally regenerated in other areas of the zone ablated by an excimer laser where the epithelium healed promptly. A persistent epithelial defect in the cornea results in the development of myofibroblasts and disordered extracellular matrix produced by these cells that together cause opacity within, and a short distance beyond, the persistent epithelial defect. Clinicians should treat persistent epithelial defects within 10 days of non-closure of the epithelium to facilitate epithelial healing to prevent long-term stromal scarring (fibrosis). [J Refract Surg. 2018;34(1):59-64.]. Copyright 2018, SLACK Incorporated.

Lithology of the basement underlying the Campi Flegrei caldera: Volcanological and petrological constraints

NASA Astrophysics Data System (ADS)

D'Antonio, Massimo

2011-02-01

A geologically reasonable working hypothesis is proposed for the lithology of the basement underlying the Campi Flegrei caldera in the ca. 4-8 km depth range. In most current geophysical modeling, this portion of crust is interpreted as composed of Meso-Cenozoic carbonate rocks, underlain by a ca. 1 km thick sill of partially molten rock, thought to be a main magma reservoir. Shallower magma reservoirs likely occur in the 3-4 km depth range. However, the lack of carbonate lithics in any Campi Flegrei caldera volcanic rocks does not support the hypothesis of a limestone basement. Considering the major caldera-forming eruptions, which generated widespread and voluminous ignimbrites during late Quaternary times, including the Campanian Ignimbrite and Neapolitan Yellow Tuff eruptions, the total volume of trachytic to phonolitic ejected magma is conservatively estimated at not less than 350 km 3. Results of least-squared mass-balance calculations suggest that this evolved magma formed through fractional crystallization from at least 2500 km 3 of parent shoshonitic magma, in turn derived from even more voluminous, more mafic, K-basaltic magma. Calculations suggest that shoshonitic magma, likely emplaced at ca. 8 km depth, must have crystallized about 2100 km 3 of solid material, dominated by alkali-feldspar and plagioclase, with a slightly lower amount of mafic minerals, during its route toward shallower magma reservoirs, before feeding the Campi Flegrei large-volume eruptions. The calculated volume of cumulate material, likely syenitic in composition at least in its upper portions, is more than enough to completely fill the basement volume in the 4-8 km depth range beneath the Campi Flegrei caldera, estimated at ca. 1250 km 3. Thus, it is proposed that the basement underlying the Campi Flegrei caldera below 4 km is composed mostly of crystalline igneous rocks, as for many large calderas worldwide. Syenite sensu lato would meet physical properties requirements for

Geochemical variability of the Yucatan basement: Constraints from crystalline clasts in Chicxulub impactites

NASA Astrophysics Data System (ADS)

Kettrup, B.; Deutsch, A.

2003-07-01

The 65 Ma old Chicxulub impact structure with a diameter of about 180 km is again in the focus of the geosciences because of the recently commenced drilling of the scientific well Yaxcopoil- 1. Chicxulub is buried beneath thick post-impact sediments, yet samples of basement lithologies in the drill cores provide a unique insight into age and composition of the crust beneath Yucatan. This study presents major element, Sr, and Nd isotope data for Chicxulub impact melt lithologies and clasts of basement lithologies in impact breccias from the PEMEX drill cores C-1 and Y-6, as well as data for ejecta material from the K/T boundaries at La Lajilla, Mexico, and Furlo, Italy. The impact melt lithologies have an andesitic composition with significantly varying contents of Al, Ca, and alkali elements. Their present day 87Sr/86Sr ratios cluster at about 0.7085, and 143Nd/144Nd ratios range from 0.5123 to 0.5125. Compared to the melt lithologies that stayed inside the crater, data for ejecta material show larger variations. The 87Sr/86Sr ratios range from 0.7081 for chloritized spherules from La Lajilla to 0.7151 for sanidine spherules from Furlo. The 143Nd/144Nd ratio is 0.5126 for La Lajilla and 0.5120 for the Furlo spherules. In an tCHUR(Nd)-tUR(Sr) diagram, the melt lithologies plot in a field delimited by Cretaceous platform sediments, various felsic lithic clasts and a newly found mafic fragment from a suevite. Granite, gneiss, and amphibolite have been identified among the fragments from crystalline basement gneiss. Their 87Sr/86Sr ratios range from 0.7084 to 0.7141, and their 143Nd/144Nd ratios range from 0.5121 to 0.5126. The TNdDM model ages vary from 0.7 to 1.4 Ga, pointing to different source terranes for these rocks. This leads us to believe that the geological evolution and the lithological composition of the Yucatàn basement is probably more complex than generally assumed, and Gondwanan as well as Laurentian crust may be present in the Yucatàn basement.

REACTIVITY MEASUREMENT FACILITY, UNDER CONSTRUCTION OVER MTR CANAL IN BASEMENT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

REACTIVITY MEASUREMENT FACILITY, UNDER CONSTRUCTION OVER MTR CANAL IN BASEMENT OF MTR BUILDING, TRA-603. WOOD PLANKS REST ON CANAL WALL OBSERVABLE IN FOREGROUND. INL NEGATIVE NO. 11745. Unknown Photographer, 8/20/1954 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

Transforming growth factor-beta production in anti-glomerular basement membrane disease in the rabbit.

PubMed Central

Coimbra, T.; Wiggins, R.; Noh, J. W.; Merritt, S.; Phan, S. H.

1991-01-01

The purpose of this study was to assay for the presence of collagen synthesis stimulatory activity in the kidney during immune-induced renal injury that results in severe fibrosis in both glomerular and interstitial compartments. A model of antiglomerular basement (anti-GBM) disease in the rabbit was induced on day 0 by the injection of anti-GBM antibody and renal cortex tissues were then sampled at various time points. Only conditioned media prepared from diseased renal cortical samples showed collagen synthesis stimulatory activity when tested on rabbit mesangial cells. The activity had an estimated molecular weight range of 16 to 25 kd and was neutralized by antibody to transforming growth factor-beta (TGF-beta). A standard assay for TGF-beta using a mink lung epithelial cell line confirmed the increase in TGF-beta activity in conditioned media of diseased cortex from day 7 and day 14 animals, which was not significantly activated by previous acidification. This suggests that most of the TGF-beta present in renal conditioned media was in the active form. The increase in renal cortical secretion of active TGF-beta was accompanied by increases in renal cortical TGF-beta mRNA content on days 4 and 7 after induction, with subsequent return to control levels. A similar increase in TGF-beta activity was present in nonacidified conditioned media of purified glomeruli from diseased days 7 and 14 animals, which was also accompanied by significant increases in TGF-beta mRNA. However with acidification no significant differences were noted between control and diseased samples, suggesting the presence of substantial latent TGF-beta activity in control glomerular conditioned media. These same control-conditioned media contained inhibitor activity for added exogenous TGF-beta. These results support the conclusion that the association between increased TGF-beta secretion and increased renal cortical collagen synthesis in this model is consistent with a role for this cytokine

Structure of Masuleh Shear Zone: Evidence for Early–Middle Jurassic Dextral Shear Along Paleo-Tethys Suture Zone in the Western Alborz, NW Iran

NASA Astrophysics Data System (ADS)

Moosavi, E.; Rasouli-Jamadi, F.

2018-03-01

The Paleo-Tethys suture zone in northern Iran was formed when the Paleo-Tethys Ocean, (between Gonwana-derived Alborz Microcontinent and the Turan Plate), closed during the Eocimmerian orogeny and after they collided together in the Mid-Late Triassic. The NW-striking Boghrov-Dagh basement Fault Zone that lies in the vicinity of Masuleh village and the southern boundary of Gasht Metamorphic Complex is a part of the Eocimmerian suture zone in the Western Alborz. Along this part of the suture zone, tourmaline leucogranites intruded in metamorphic rocks. We recognize three distinct deformation stages (D1 to D3) in the study area especially in the Masuleh Shear Zone. D1 which was synchronous with formation of the main metamorphic minerals, such as sillimanite and staurolite under medium- to high-grade metamorphic conditions probably during the Hercynian event and a NE-directed shortening. The slaty cleavage in metamorphosed Upper Paleozoic rocks and crenulation cleavage and folds in the older rocks were produced due to D2 deformation during the Eocimmerian event under greenschist facies conditions. The Masuleh Shear Zone formed as a result of a ductile strike-slip shear during the Early-Middle Jurassic Mid-Cimmerian D3 event with a pure dextral to transtension shear sense at low to locally medium-grade conditions. All of the D3 structural features agree with a NNW-directed compression and an ENE-directed extension caused by overall dextral shear parallel to the Masuleh shear zone and the Boghrov-Dagh Fault Zone. Based on the available evidence, especially cross-cutting relationships between structural fabrics and rock units, emplacement of the Gasht-Masuleh leucogranites occurred after the D2 collisional event coeval to the possible slab break-off and before the D3 event, between Eocimmerian and Mid-Cimmerian movements.

Ultrathin Polymer Membranes with Patterned, Micrometric Pores for Organs-on-Chips.

PubMed

Pensabene, Virginia; Costa, Lino; Terekhov, Alexander Y; Gnecco, Juan S; Wikswo, John P; Hofmeister, William H

2016-08-31

The basal lamina or basement membrane (BM) is a key physiological system that participates in physicochemical signaling between tissue types. Its formation and function are essential in tissue maintenance, growth, angiogenesis, disease progression, and immunology. In vitro models of the BM (e.g., Boyden and transwell chambers) are common in cell biology and lab-on-a-chip devices where cells require apical and basolateral polarization. Extravasation, intravasation, membrane transport of chemokines, cytokines, chemotaxis of cells, and other key functions are routinely studied in these models. The goal of the present study was to integrate a semipermeable ultrathin polymer membrane with precisely positioned pores of 2 μm diameter in a microfluidic device with apical and basolateral chambers. We selected poly(l-lactic acid) (PLLA), a transparent biocompatible polymer, to prepare the semipermeable ultrathin membranes. The pores were generated by pattern transfer using a three-step method coupling femtosecond laser machining, polymer replication, and spin coating. Each step of the fabrication process was characterized by scanning electron microscopy to investigate reliability of the process and fidelity of pattern transfer. In order to evaluate the compatibility of the fabrication method with organs-on-a-chip technology, porous PLLA membranes were embedded in polydimethylsiloxane (PDMS) microfluidic devices and used to grow human umbilical vein endothelial cells (HUVECS) on top of the membrane with perfusion through the basolateral chamber. Viability of cells, optical transparency of membranes and strong adhesion of PLLA to PDMS were observed, thus confirming the suitability of the prepared membranes for use in organs-on-a-chip devices.

15. ELECTRICAL REACTOR SHELVES, CONSTRUCTED OF CONCRETE IN THE BASEMENT ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

15. ELECTRICAL REACTOR SHELVES, CONSTRUCTED OF CONCRETE IN THE BASEMENT ALONG EAST WALL, WITH REACTOR PADS BEHIND FRAMED AND SCREENED CAGE, AND PORCELAIN-LINED CABLE DUCTS VISIBLE IN WALL NEAR FLOOR AT REAR - Bonneville Power Administration South Bank Substation, I-84, South of Bonneville Dam Powerhouse, Bonneville, Multnomah County, OR

New process for alleviation of membrane fouling of modified hybrid MBR system for advanced domestic wastewater treatment.

PubMed

Shuo, Liu; Baozhen, Wang; Hongjun, Han; Yanping, Liu

2008-01-01

A pilot-scale hybrid membrane bioreactor using a submerged flat panel membrane was designed and applied for advanced treatment of domestic wastewater. The new process adapted to the hybrid membrane bioreactor exhibits substantial decrease in membrane fouling and much easier cleaning. In this study, the new process configurations including the addition of anoxic/anaerobic zones, the package of synthetic fibrous fabric carrier for biofilm attached growth, activated sludge recycling and modified dosage of polished diatomite with high activity and multi-functions were investigated to select the optimal operational parameters for the hybrid membrane bioreactor system. The carrier package in the aerobic zone contributed 3.65 g/L (maximum) of fixed biomass to the system, thus reducing the suspended biomass, and has decreased the membrane cleaning cycle remarkably. The operation performance at the sludge recycle rate 0, 100%, 200% and 300% showed that, the trans-membrane pressure of flat panel membrane declined sharply with the increase of sludge recycling rate within a certain range, and 200% was decided to be optimal for in the membrane bioreactor system. EPS concentration in each sludge recycling rate was 135 mg/L, 92 mg/L, 68 mg/L and 55 mg/L respectively. The addition of anoxic and anaerobic zones degraded some large molecular organic compounds, which facilitated the biodegradation and removal of organic substances in aerobic zone. The modified dosage of polished diatomite has played a major important role for both preventing of membrane from fouling and its much easier cleaning when it formed. Copyright (c) IWA Publishing 2008.

Modeling the Proterozoic Basement's Effective Stress Field, Assessing Fault Reactivation Potential Related to Increased Fluid Pressures, and Improved 3D Structural Interpretation of Faulting within Wellington and Anson-Bates Fields, Sumner County, Kansas

NASA Astrophysics Data System (ADS)

Keast, R. T.; Lacroix, B.; Raef, A. E.; Adam, C.; Bidgoli, T. S.; Leclere, H.; Daniel, G.

2017-12-01

South-central Kansas has experienced an increase in seismic activity within the Proterozoic basement. Since 2013, United States Geological Survey (USGS) seismograph stations have recorded 3414 earthquakes. Fluid pressure increases associated with recent high-rate wastewater injection into the dolomitic Arbuckle disposal zone is the hypothesized cause of reactivation of the faulted study region's Proterozoic basement. Although the magnitude of the pressure change required for reactivation of these faults is likely low given failure equilibrium conditions in the midcontinent, heterogeneities in the basement could allow for a range of fluid pressure changes associated with injection. This research aims to quantify the fluid pressure changes responsible for fault reactivation of the Proterozoic basement. To address this issue, we use 103 focal mechanisms and 3,414 seismic events, from the USGS catalog, within an area encompassing 4,000 km2. Three major fault populations have been identified using the dense seismicity and focal mechanism datasets. Win-Tensor paleostress reconstruction software was used to identify effective stress ratios, R = (σ'1/σ'3), and stress tensors for twelve 22 km by 17 km grid squares covering the study area. One fault population strikes parallel with the Nemaha Ridge basement structure ( 030˚). Another reoccurring fault population is oriented 310˚, closely parallel to the Central Kansas Uplift, a subtle anticlinal structure subjected to repeated movement during the Paleozoic. The third population of faults is parallel to the regional maximum compressive stress oriented 265˚ as determined by previous researchers using borehole image logs and shear wave anisotropy. A 3D stress modeling Matlab script was used to analyze fault reactivation potential based on results obtained from Win-Tensor to better understand fault orientations and their susceptibility to reactivation related to pore fluid pressure increases. In addition, the orientations

Geothermal energy prospectivity of the Torrens Hinge Zone: evidence from new heat flow data

NASA Astrophysics Data System (ADS)

Matthews, Chris

2009-09-01

The Torrens Hinge Zone is a long but narrow (up to 40km wide) geological transition zone between the relatively stable Eastern Gawler Craton `Olympic Domain' to the west, and the sedimentary basin known as the Adelaide Geosyncline to the east. The author hypothesised from first principles that the Torrens Hinge Zone should be prospective for high geothermal gradients due to the likely presence of high heat flow and insulating cover rocks. A method to test this hypothesis was devised, which involved the determination of surface heat flow on a pattern grid using purpose-drilled wells, precision temperature logging and detailed thermal conductivity measurements. The results of this structured test have validated the hypothesis, with heat flow values over 90mW/m2 recorded in five of six wells drilled. With several kilometres thickness of moderate conductivity sediments overlying the crystalline basement in this region, predicted temperature at 5000m ranges between 200 and 300°C.

ELKS active zone proteins as multitasking scaffolds for secretion

PubMed Central

Held, Richard G.

2018-01-01

Synaptic vesicle exocytosis relies on the tethering of release ready vesicles close to voltage-gated Ca2+ channels and specific lipids at the future site of fusion. This enables rapid and efficient neurotransmitter secretion during presynaptic depolarization by an action potential. Extensive research has revealed that this tethering is mediated by an active zone, a protein dense structure that is attached to the presynaptic plasma membrane and opposed to postsynaptic receptors. Although roles of individual active zone proteins in exocytosis are in part understood, the molecular mechanisms that hold the protein scaffold at the active zone together and link it to the presynaptic plasma membrane have remained unknown. This is largely due to redundancy within and across scaffolding protein families at the active zone. Recent studies, however, have uncovered that ELKS proteins, also called ERC, Rab6IP2 or CAST, act as active zone scaffolds redundant with RIMs. This redundancy has led to diverse synaptic phenotypes in studies of ELKS knockout mice, perhaps because different synapses rely to a variable extent on scaffolding redundancy. In this review, we first evaluate the need for presynaptic scaffolding, and we then discuss how the diverse synaptic and non-synaptic functional roles of ELKS support the hypothesis that ELKS provides molecular scaffolding for organizing vesicle traffic at the presynaptic active zone and in other cellular compartments. PMID:29491150

Structural evolution of the Sarandí del Yí Shear Zone, Uruguay: kinematics, deformation conditions and tectonic significance

NASA Astrophysics Data System (ADS)

Oriolo, S.; Oyhantçabal, P.; Heidelbach, F.; Wemmer, K.; Siegesmund, S.

2015-10-01

The Sarandí del Yí Shear Zone is a crustal-scale shear zone that separates the Piedra Alta Terrane from the Nico Pérez Terrane and the Dom Feliciano Belt in southern Uruguay. It represents the eastern margin of the Río de la Plata Craton and, consequently, one of the main structural features of the Precambrian basement of Western Gondwana. This shear zone first underwent dextral shearing under upper to middle amphibolite facies conditions, giving rise to the reactivation of pre-existing crustal fabrics in the easternmost Piedra Alta Terrane. Afterwards, pure-shear-dominated sinistral shearing with contemporaneous magmatism took place under lower amphibolite to upper greenschist facies conditions. The mylonites resulting from this event were then locally reactivated by a cataclastic deformation. This evolution points to strain localization under progressively retrograde conditions with time, indicating that the Sarandí del Yí Shear Zone represents an example of a thinning shear zone related to the collisional to post-collisional evolution of the Dom Feliciano Belt that occurred between the Meso- to Neoproterozoic (>600 Ma) and late Ediacaran-lower Cambrian times.

5. INTERIOR VIEW OF BASEMENT, LOOKING WEST. DEEP DRAW, HEAVY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. INTERIOR VIEW OF BASEMENT, LOOKING WEST. DEEP DRAW, HEAVY PRESS MANUFACTURED BY E. W. BLISS CO., BROOKLYN, NEW YORK. PRESS #3-3/4 S, PATENTED BY E. W. BLISS CO., AUGUST 16, 1892, AND JANUARY 31, 1893. THIS PRESS DATES FROM CA. 1920. - Illinois Pure Aluminum Company, 109 Holmes Street, Lemont, Cook County, IL

Magnetotelluric survey to locate the Archean/Proterozoic suture zone north of Wells, Nevada

USGS Publications Warehouse

Williams, Jackie M.; Rodriguez, Brian D.

2006-01-01

It is important to know whether major mining districts in the Northern Nevada Gold Province are underlain by rocks of the Archean Wyoming craton, which are known to contain orogenic gold deposits, or by accreted rocks of the Paleoproterozoic Mojave province. It is also important to know the location and orientation of the Archean/Proterozoic suture zone between these provinces as well as major basement structures within these terranes because they may influence subsequent patterns of sedimentation, deformation, magmatism, and hydrothermal activity. The Archean was the main gold-mineralization period, and Archean lode-gold deposits were formed at mid-crustal depths along major shear zones. The nature of the crystalline basement below the Northern Nevada Gold Province and the location of major faults within it are relevant to Rodinian reconstructions, crustal development, and ore deposit models (e.g., Hofstra and Cline, 2000; Grauch and others, 2003). According to Whitmeyer and Karlstrom (2004), the Archean cratons of the northwestern United States and Canada had stabilized as continental lithosphere by 2.5 Ga, and were rifted and assembled into a large continental mass by 1.8 Ga, to which the 1.73-1.68 Ga Mohave province was accreted by 1.65 Ga. The Archean/Proterozoic suture zone has a west-southwest strike where it is exposed (Reed, 1993) at the eastern Utah and southwestern Wyoming border (Cheyenne Belt) where it is characterized by an up to 7-km-thick mylonite zone (Smithson and Boyd, 1998). In the Great Basin, the strike of the Archean/Proterozoic suture zone is poorly constrained because it is largely concealed below a Neoproterozoic-Paleozoic miogeocline and basin fill. East-west and southwest-northeast strikes for the Archean/Proterozoic suture zone have been inferred based on Sr, Nd, and Pb isotopic compositions of granitoid intrusions (Tosdal and others, 2000). To better constrain the location and strike of the Archean/Proterozoic suture zone below cover

ETR BUILDING, TRA642, INTERIOR. BASEMENT. ROLLUP DOOR TO CUBICLE POSTS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

ETR BUILDING, TRA-642, INTERIOR. BASEMENT. ROLLUP DOOR TO CUBICLE POSTS CAUTION SIGNS BECAUSE OF SODIUM HAZARD WITHIN. INL NEGATIVE NO. HD24-3-1. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

MTR WING, TRA604, INTERIOR. BASEMENT. WEST CORRIDOR. CAMERA FACES NORTH. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

MTR WING, TRA-604, INTERIOR. BASEMENT. WEST CORRIDOR. CAMERA FACES NORTH. HVAC AREA IS AT RIGHT OF CORRIDOR. INL NEGATIVE NO. HD46-13-3. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

PBF Reactor Building (PER620). Camera in first basement, facing south ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF Reactor Building (PER-620). Camera in first basement, facing south and upward toward main floor. Cable trays being erected. Photographer: Kirsh. Date: May 20, 1969. INEEL negative no. 69-3110 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

PBF (PER620) interior, basement level. Concrete wall shows outline of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF (PER-620) interior, basement level. Concrete wall shows outline of reactor basin. Sign says, "Flashing Light - Reactor On - Evacuate Area." Date: May 2004. INEEL negative no. HD-41-5-3 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

ETR BUILDING, TRA642, INTERIOR. BASEMENT. LIQUID SODIUM PIPING INSIDE CUBICLE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

ETR BUILDING, TRA-642, INTERIOR. BASEMENT. LIQUID SODIUM PIPING INSIDE CUBICLE SHOWN IN ID-33-G-101. INL NEGATIVE NO. HD24-3-4. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

Numerical analysis of temperature distribution due to basement radiogenic heat production, St. Lawrence Lowlands, eastern Canada

NASA Astrophysics Data System (ADS)

Liu, Hejuan; Giroux, Bernard; Harris, Lyal B.; Mansour, John

2017-04-01

Although eastern Canada is considered as having a low potential for high-temperature geothermal resources, the possibility for additional localized radioactive heat sources in Mesoproterozoic Grenvillian basement to parts of the Palaeozoic St. Lawrence Lowlands in Quebec, Canada, suggests that this potential should be reassessed. However, such a task remains hard to achieve due to scarcity of heat flow data and ambiguity about the nature of the basement. To get an appraisal, the impact of radiogenic heat production for different Grenville Province crystalline basement units on temperature distribution at depth was simulated using the Underworld Geothermal numerical modelling code. The region south of Trois-Rivières was selected as representative for the St. Lawrence Lowlands. An existing 3D geological model based on well log data, seismic profiles and surface geology was used to build a catalogue of plausible thermal models. Statistical analyses of radiogenic element (U, Th, K) concentrations from neighbouring outcropping Grenville domains indicate that the radiogenic heat production of rocks in the modelled region is in the range of 0.34-3.24 μW/m3, with variations in the range of 0.94-5.83 μW/m3 for the Portneuf-Mauricie (PM) Domain, 0.02-4.13 μW/m3 for the Shawinigan Domain (Morin Terrane), and 0.34-1.96 μW/m3 for the Parc des Laurentides (PDL) Domain. Various scenarios considering basement characteristics similar to the PM domain, Morin Terrane and PDL Domain were modelled. The results show that the temperature difference between the scenarios can be as much as 12 °C at a depth of 5 km. The results also show that the temperature distribution is strongly affected by both the concentration of radiogenic elements and the thermal conductivity of the basement rocks. The thermal conductivity in the basement affects the trend of temperature change between two different geological units, and the spatial extent of thermal anomalies. The validity of the results was

11. VIEW NORTH OF MACHINE SHOP IN BASEMENT OF OLD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. VIEW NORTH OF MACHINE SHOP IN BASEMENT OF OLD POWERHOUSE, WITH PIPECUTTER (LEFT), DRILL PRESS LEFT (CENTER), AND GRINDER (RIGHT CENTER) BENEATH LINE SHAFTING) - Trenton Falls Hydroelectric Station, Powerhouse & Substation, On west bank of West Canada Creek, along Trenton Falls Road, 1.25 miles north of New York Route 28, Trenton Falls, Oneida County, NY

7. INTERIOR VIEW OF BASEMENT OF CA. 1948 FACTORY ADDITION, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. INTERIOR VIEW OF BASEMENT OF CA. 1948 FACTORY ADDITION, LOOKING SOUTHEAST. AT CENTER IS A DEEP-BRAWN, HEAVY PRESS MANUFACTURED BY E. W. BLISS CO., BROOKLYN, NEW YORK. PRESS #3-1/2-C PATENTED BY E. W. BLISS CO., 1893. MANUFACTURER'S PLATE INDICATES PRESS DATES FROM 1922. - Illinois Pure Aluminum Company, 109 Holmes Street, Lemont, Cook County, IL

Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue.

PubMed

Holsti, Mari; Wanhainen, Anders; Lundin, Christina; Björck, Martin; Tegler, Gustaf; Svensson, Johan; Sund, Malin

2018-04-12

Abdominal aortic aneurysm (AAA) is characterised by enhanced proteolytic activity, and extracellular matrix (ECM) remodelling in the vascular wall. Type IV and XVIII collagen/endostatin are structural proteins in vascular basement membrane (VBM), a specialised ECM structure. Here the association between plasma levels of these collagens with the aortic diameter and expansion rate is studied, and their expression in aortic tissue characterised. This was a retrospective population based cohort study. Type IV and XVIII collagen/endostatin were analysed in plasma by ELISA assay in 615 men, divided into three groups based on the aortic diameter: 1) normal aorta ≤ 25 mm, 2) sub-aneurysmal aorta (SAA) 26-29 mm, and 3) AAA ≥ 30 mm. Follow up data were available for 159 men. The association between collagen levels and aortic diameter at baseline, and with the expansion rate at follow up were analysed in ordinal logistic regression and linear regression models, controlling for common confounding factors. Tissue expression of the collagens was analysed in normal aorta (n = 6) and AAA (n = 6) by immunofluorescence. Plasma levels of type XVIII collagen/endostatin (136 ng/mL [SD 29] in individuals with a normal aorta diameter, 154 ng/ml [SD 45] in SAA, and 162 ng/ml [SD 46] in AAA; p = .001) and type IV collagen (105 ng/mL [SD 42] normal aorta, 124 ng/ml [SD 46] SAA, and 127 ng/ml [SD 47] AAA; p = .037) were associated with a larger aortic diameter. A significant association was found between the baseline levels of type XVIII/endostatin and the aortic expansion rate (p = .035), but in the multivariable model, only the initial aortic diameter remained significantly associated with expansion (p = .005). Altered expression patterns of both collagens were observed in AAA tissue. Plasma levels of circulating type IV and XVIII collagen/endostatin increase with AAA diameter. The expression pattern of VBM proteins is altered in the aneurysm wall. Copyright �

31. VIEW EAST IN BASEMENT OF BUILDING 41A; NOTE REMAINS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. VIEW EAST IN BASEMENT OF BUILDING 41A; NOTE REMAINS OF SUPPORTS FOR OVERHEAD TROLLEY SYSTEM AT LEFT CENTER OF PHOTOGRAPH; TROLLEY SYSTEM WAS USED TO CARRY BASKETS OF SMALL PARTS ALONG THE ELECTROPLATING LINE - Scovill Brass Works, 59 Mill Street, Waterbury, New Haven County, CT

PBF (PER620) interior, first basement. Detail of valves and other ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF (PER-620) interior, first basement. Detail of valves and other penetrations along wall. Bricks are made of high density shielding materials. Date: March 2004. INEEL negative no. HD-41-4-2 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

Localization of sialic acid in kidney glomeruli: regionalization in the podocyte plasma membrane and loss in experimental nephrosis.

PubMed

Charest, P M; Roth, J

1985-12-01

Sialic acid residues were localized by electron microscopy in renal glomeruli of normal and puromycin-treated rats with a cytochemical technique that utilized the Limax flavus lectin. In Lowicryl K4M thin sections from normal rats, sialic acid residues were found along the plasma membrane of the various glomerular cell types and in the glomerular basement membrane as well as the mesangial matrix. In NaDodSO4/PAGE, sialic acid residues of normal glomeruli were mainly confined to a 140-kDa protein previously identified as podocalyxin. The distribution of sialic acid residues in the podocyte plasma membrane was found to be remarkably regionalized. Based on the differential labeling intensity, three plasma membrane domains could be defined: the foot process base, the foot process region above the slit diaphragm, and the body of podocytes. Cytochemical and biochemical analysis of glomeruli from puromycin-treated rats showed a loss of sialic acid residues from glomerular sialoglycoconjugates indicating a perturbated glycosylation.

Lithologies of the basement complex (Devonian and older) in the National Petroleum Reserve - Alaska

USGS Publications Warehouse

Dumoulin, Julie A.; Houseknecht, David W.

2001-01-01

Rocks of the basement complex (Devonian and older) were encountered in at least 30 exploratory wells in the northern part of the NPRA. Fine-grained, variably deformed sedimentary rocks deposited in a slope or basinal setting predominate and include varicolored (mainly red and green) argillite in the Simpson area, dark argillite and chert near Barrow, and widespread gray argillite. Chitinozoans of Middle-Late Ordovician and Silurian age occur in the dark argillite and chert unit. Sponge spicules and radiolarians establish a Phanerozoic age for the varicolored and gray argillite units, both of which contain local interbeds of chert-rich sandstone and silt-stone. Conglomerate and sandstone, also chert-rich but interbedded with mudstone and coal and of Early-Middle Devonian age, occur in the Topagoruk area; these strata formed in a fluvial environment. At East Teshekpuk, granite of probable Devonian age was penetrated. Brecciated, quartz-veined rock of uncertain protolith that may be part of the basement complex was encountered in the Ikpikpuk well. Seismic data indicate that angular unconformities truncate all sedimentary units of the basement complex in NPRA. Rocks correlative in age and lithofacies with the dark argillite and chert unit occur in the subsurface near Prudhoe Bay. Other argillite units in NPRA have similarities to basement rocks in the subsurface adjacent to ANWR and the Ordovician-Silurian Iviagik Group at Cape Lisburne, but lack the interbedded limestones found in the ANWR strata, and are less metamorphosed than, and compositionally distinct from, the Iviagik. The Topagoruk conglomerate and the East Teshekpuk granite resemble the Ulungarat formation and the Okpilak batholith, respectively, in the northeastern Brooks Range.

Halogens are key cofactors in building of collagen IV scaffolds outside the cell.

PubMed

Brown, Kyle L; Hudson, Billy G; Voziyan, Paul A

2018-05-01

The purpose of this review is to highlight recent advances in understanding the molecular assembly of basement membranes, as exemplified by the glomerular basement membrane (GBM) of the kidney filtration apparatus. In particular, an essential role of halogens in the basement membrane formation has been discovered. Extracellular chloride triggers a molecular switch within non collagenous domains of collagen IV that induces protomer oligomerization and scaffold assembly outside the cell. Moreover, bromide is an essential cofactor in enzymatic cross-linking that reinforces the stability of scaffolds. Halogenation and halogen-induced oxidation of the collagen IV scaffold in disease states damage scaffold function. Halogens play an essential role in the formation of collagen IV scaffolds of basement membranes. Pathogenic damage of these scaffolds by halogenation and halogen-induced oxidation is a potential target for therapeutic interventions.

PBF (PER620) interior, second basement level. Coolant and tank piping. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF (PER-620) interior, second basement level. Coolant and tank piping. Mark on vertical pipe says, "H.P. Demin. Water." (High pressure demineralized water.) Date: March 2004. INEEL negative no. HD-41-4-3 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

Isotopic, geochemical, and temporal characterization of Proterozoic basement rocks in the Quitovac region, northwestern Sonora, Mexico: Implications for the reconstruction of the southwestern margin of Laurentia

USGS Publications Warehouse

Iriondo, A.; Premo, W.R.; Martínez-Torres, Luis M.; Budahn, J.R.; Atkinson, William W.; Siems, D.F.; Guaras-Gonzalez, B.

2004-01-01

A detailed geochemical characterization of 19 representative Proterozoic basement rocks in the Quitovac region in northwestern Sonora, Mexico, has identified two distinct Paleoproterozoic basement blocks that coincide spatially with the previously proposed Caborca and "North America" blocks. New U-Pb zircon geochronology revises their age ranges, the Caborca (1.78-1.69 Ga) and "North America" (1.71-1.66 Ga) blocks at Quitovac, and precludes a simple age differentiation between them. In addition, Grenvillian-age granitoids (ca. 1.1 Ga), spatially associated with the Caborca block have been identified at Quitovac. Nd isotopes and major- and trace-element geochemistry support the distinction of these Paleoproterozoic blocks. Granitoids of the "North America" block are characterized by depleted ??Nd values (3.4-3.9) and younger Nd model ages (1800-1740 Ma) and have lower K2O, Y, Rb, Ba, Th, REE, and Fe/Mg values than coeval rocks of the Caborca block. The Caborca block granitoids are likewise characterized by slightly less depleted ??Nd (0.6-2.6) and older Nd model ages (2070-1880 Ma). Despite the subtle differences, granitoids from both the Caborca and "North America" blocks exhibit island arc-like affinities. We propose that the Proterozoic basement rocks from the Quitovac region are an extension of the Proterozoic crustal provinces in the southwestern United States. Specifically, rocks of the Caborca block exhibit an affinity to rocks of either the Yavapai province or the Mojave-Yavapai transition zone, whereas rocks of the "North America" block have signatures similar to those of the Mazatzal province or possibly the Yavapai province of Arizona. The new isotopic ages and geochemical data do not support the existence of the Late Jurassic Mojave-Sonora megashear at Quitovac, as originally proposed. However, the Quitovac region accounts only for a small fraction of the Proterozoic basement in Sonora, so these findings do not eliminate the possibility of a megashear

Studies on improved integrated membrane-based chromatographic process for bioseparation

NASA Astrophysics Data System (ADS)

Xu, Yanke

To improve protein separation and purification directly from a fermentation broth, a novel membrane filtration-cum-chromatography device configuration having a relatively impermeable coated zone near the hollow fiber module outlet has been developed. The integrated membrane filtration-cum-chromatography unit packed with chromatographic beads on the shell side of the hollow fiber unit enjoys the advantages of both membrane filtration and chromatography; it allows one to load the chromatographic media directly from the fermentation broth or lysate and separate the adsorbed proteins through the subsequent elution step in a cyclic process. Interfacial polymerization was carried out to coat the bottom section of the hollow fiber membrane while leaving the rest of the hollow fiber membrane unaffected. Myoglobin (Mb), bovine serum albumin (BSA) and a-lactalbumin (a-LA) were used as model proteins in binary mixtures. Separation behaviors of binary protein mixtures were studied in devices using either an ultrafiltration (UF) membrane or a microfiltration (MF) membrane. Experimental results show that the breakthrough time and the protein loading capacities were dramatically improved after coating in both UF and MF modules. For a synthetic yeast fermentation broth feed, the Mb and a-LA elution profiles for the four consecutive cyclic runs were almost superimposable. Due to the lower transmembrane flux in this device plus the periodical washing-elution during the chromatographic separation, fouling was not a problem as it is in conventional microfiltration. A mathematical model describing the hydrodynamic and protein loading behaviors of the integrated device using UF membrane with a coated zone was developed. The simulation results for the breakthrough agree well with the experimental breakthrough curves. The optimal length of the coated zone was obtained from the simulation. A theoretical analysis of the protein mass transfer was performed using a diffusion-convection model

Age of Izu-Bonin-Mariana arc basement

NASA Astrophysics Data System (ADS)

Ishizuka, Osamu; Hickey-Vargas, Rosemary; Arculus, Richard J.; Yogodzinski, Gene M.; Savov, Ivan P.; Kusano, Yuki; McCarthy, Anders; Brandl, Philipp A.; Sudo, Masafumi

2018-01-01

Documenting the early tectonic and magmatic evolution of the Izu-Bonin-Mariana (IBM) arc system in the Western Pacific is critical for understanding the process and cause of subduction initiation along the current convergent margin between the Pacific and Philippine Sea plates. Forearc igneous sections provide firm evidence for seafloor spreading at the time of subduction initiation (52 Ma) and production of "forearc basalt". Ocean floor drilling (International Ocean Discovery Program Expedition 351) recovered basement-forming, low-Ti tholeiitic basalt crust formed shortly after subduction initiation but distal from the convergent margin (nominally reararc) of the future IBM arc (Amami Sankaku Basin: ASB). Radiometric dating of this basement gives an age range (49.3-46.8 Ma with a weighted average of 48.7 Ma) that overlaps that of basalt in the present-day IBM forearc, but up to 3.3 m.y. younger than the onset of forearc basalt activity. Similarity in age range and geochemical character between the reararc and forearc basalts implies that the ocean crust newly formed by seafloor spreading during subduction initiation extends from fore- to reararc of the present-day IBM arc. Given the age difference between the oldest forearc basalt and the ASB crust, asymmetric spreading caused by ridge migration might have taken place. This scenario for the formation of the ASB implies that the Mesozoic remnant arc terrane of the Daito Ridges comprised the overriding plate at subduction initiation. The juxtaposition of a relatively buoyant remnant arc terrane adjacent to an oceanic plate was more favourable for subduction initiation than would have been the case if both downgoing and overriding plates had been oceanic.

Membrane-lined foundations for liquid thermal storage

NASA Astrophysics Data System (ADS)

Bourne, R. C.

1981-06-01

The membrane lined storage (MLS) container which is a spinoff of vinyl-lined swimming pool and waterbed technologies was developed. The state of development of MLS was evaluated and concepts for MLS structural and heat transfer systems were improved. Preferred structural supports were identified and designed for 1500 gal MLS containers for basement, crawl space, and slab-on-grade foundation types. Techniques are developed to provide space heating via forced air through a finned storage jacket for the two preferred structural enclosure designs. Cost effectiveness of the direct air heating technique is evaluated. Alternate free convection domestic water preheaters and a preferred heat exchanger material is selected. Collector and space heat inlet/outlet designs, design concepts for auxiliary heat input to MLS from resistance electric, combustion, and heat pump sources are developed.

MTR BASEMENT. WORKERS (DON ALVORD AND CYRIL VAN ORDEN OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

MTR BASEMENT. WORKERS (DON ALVORD AND CYRIL VAN ORDEN OF PHILLIPS PETROLEUM CO.) POSE FOR GAMMA IRRADIATION EXPERIMENT IN MTR CANAL. CANS OF FOOD WILL BE LOWERED TO CANAL BOTTOM, WHERE SPENT MTR FUEL ELEMENTS EMIT GAMMA RADIATION. INL NEGATIVE NO. 11746. Unknown Photographer, 8/20/1954 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

PBF Reactor Building (PER620). Camera in second basement near subpile ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF Reactor Building (PER-620). Camera in second basement near sub-pile room (directly below reactor vessel). Door and penetrations lead to sub-pile room. Date: August 15, 1969. Photographer: Larry Page. INEEL negative no. 69-4310 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP601) BASEMENT SHOWING PROCESS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP-601) BASEMENT SHOWING PROCESS CORRIDOR AND EIGHTEEN CELLS. TO LEFT IS LABORATORY BUILDING (CPP-602). INL DRAWING NUMBER 200-0601-00-706-051981. ALTERNATE ID NUMBER CPP-E-1981. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID

Precambrian basement geology of North and South Dakota.

USGS Publications Warehouse

Klasner, J.S.; King, E.R.

1986-01-01

Combined analysis of drill-hole, gravity and magnetic data indicates that the Precambrian rocks in the basement of the Dakotas may be divided into a series of lithotectonic terrains. On the basis of an analysis of geological and geophysical data in the Dakotas and from the surrounding states and Canada, it is shown how the exposed Precambrian rocks of the adjacent shield areas project into the study area. Brief comments are made on the tectonic implications of this study. Geological and geophysical characteristics of 11 terrains are tabulated. -P.Br.

Fusion competent synaptic vesicles persist upon active zone disruption and loss of vesicle docking

PubMed Central

Wang, Shan Shan H.; Held, Richard G.; Wong, Man Yan; Liu, Changliang; Karakhanyan, Aziz; Kaeser, Pascal S.

2016-01-01

In a nerve terminal, synaptic vesicle docking and release are restricted to an active zone. The active zone is a protein scaffold that is attached to the presynaptic plasma membrane and opposed to postsynaptic receptors. Here, we generated conditional knockout mice removing the active zone proteins RIM and ELKS, which additionally led to loss of Munc13, Bassoon, Piccolo, and RIM-BP, indicating disassembly of the active zone. We observed a near complete lack of synaptic vesicle docking and a strong reduction in vesicular release probability and the speed of exocytosis, but total vesicle numbers, SNARE protein levels, and postsynaptic densities remained unaffected. Despite loss of the priming proteins Munc13 and RIM and of docked vesicles, a pool of releasable vesicles remained. Thus, the active zone is necessary for synaptic vesicle docking and to enhance release probability, but releasable vesicles can be localized distant from the presynaptic plasma membrane. PMID:27537483

Effect of Fibrin Glue on the Biomechanical Properties of Human Descemet's Membrane

PubMed Central

Chaurasia, Shyam S.; Champakalakshmi, Ravi; Li, Ang; Poh, Rebekah; Tan, Xiao Wei; Lakshminarayanan, Rajamani; Lim, Chwee T.; Tan, Donald T.; Mehta, Jodhbir S.

2012-01-01

Background Corneal transplantation has rapidly evolved from full-thickness penetrating keratoplasty (PK) to selective tissue corneal transplantation, where only the diseased portions of the patient's corneal tissue are replaced with healthy donor tissue. Descemet's membrane endothelial keratoplasty (DMEK) performed in patients with corneal endothelial dysfunction is one such example where only a single layer of endothelial cells with its basement membrane (10–15 µm in thickness), Descemet's membrane (DM) is replaced. It is challenging to replace this membrane due to its intrinsic property to roll in an aqueous environment. The main objective of this study was to determine the effects of fibrin glue (FG) on the biomechanical properties of DM using atomic force microscopy (AFM) and relates these properties to membrane folding propensity. Methodology/Principal Findings Fibrin glue was sprayed using the EasySpray applicator system, and the biomechanical properties of human DM were determined by AFM. We studied the changes in the “rolling up” tendency of DM by examining the changes in the elasticity and flexural rigidity after the application of FG. Surface topography was assessed using scanning electron microscopy (SEM) and AFM imaging. Treatment with FG not only stabilized and stiffened DM but also led to a significant increase in hysteresis of the glue-treated membrane. In addition, flexural or bending rigidity values also increased in FG-treated membranes. Conclusions/Significance Our results suggest that fibrin glue provides rigidity to the DM/endothelial cell complex that may aid in subsequent manipulation by maintaining tissue integrity. PMID:22662156

TESTING OF INDOOR RADON REDUCTION TECHNIQUES IN BASEMENT HOUSES HAVING ADJOINING WINGS

EPA Science Inventory

The report gives results of tests of indoor radon reduction techniques in 12 existing Maryland houses, with the objective of determining when basement houses with adjoining wings require active soil depressurization (ASD) treatment of both wings, and when treatment of the basemen...

Metasomatic tourmalinite formation along basement-cover decollements, Orobic Alps, Italy

USGS Publications Warehouse

Slack, J.F.; Passchier, C.W.; Zhang, J.S.

1996-01-01

Cryptocrystalline tourmalinites that occur discontinuously for ???30 km along basement-cover de??collements of the Orohic Alps (Italy) formed by the metasomatism of aluminous cataclasites derived from Permian conglomerates and/or feldspathic sandstones. Using Al as an immobile element monitor, calculations show that the majority of tourmalinites in the region formed through the addition of moderate to significant amounts of B, Mg, Na, Sr, and Be, and the loss of moderate to significant Mn, Ca, K, P, Rb, Ba, and Cr; minor Si, Ti, V, light REE, and Eu also were lost. Data tor relatively immobile Al, Zr, Th, Sc, Nb, and heavy REE indicate that, on average, these tourmalinites formed through ???12% net mass loss assuming an original conglomerate protolith, or through ???7% net mass loss assuming a sandstone protolith. The B and other introduced constituents in the tourmalinites were deposited by hydrothermal fluids focused along and near basement-cover de??collements. These fluids, believed to be associated with late Hercynian felsic magmatism, probably are related to fluids that formed the tourmaline-rich U-Mo-Zn deposits at the nearby Novazza mine and the U-Zn deposits at the nearby Val Vedello mine.

Paleohighs and Paleolows in the Basement Rocks of the Eastern Gulf of Mexico

NASA Astrophysics Data System (ADS)

Robinson, D.; Weislogel, A. L.

2017-12-01

The Eastern Gulf of Mexico has topography on the basement rocks composed of igneous and metamorphic rocks as well as some sedimentary rocks underneath a relatively thin salt layer with 3-6 km of topography relief. Paleohighs from south to north include Sarasota Arch, Middle Ground Arch/Southern Platform, Pensacola Arch, Conecuh Ridge Complex, Baldwin High, Wiggins Arch and Choctaw Ridge Complex. Paleolows from south to north include South Florida Basin, Tampa Embayment, Apalachicola Basin/Desoto Canyon Salt Basin, Conecuh Embayment, Manila Embayment and the Mississippi Interior Salt Basin. The topography on the basement is a result of several collisions between Laurentian and Gondwana to produce Pangea with final suturing during Pennsylvanian time and also from extension in Late Triassic to Early Cretaceous time as a result of the opening of the Gulf and rotation of Yucatan. Heterogeneities related to previous collisions may have also factored into producing these paleohighs and paleolows. A series of grabens and half-grabens, trending northeast-southwest from northwest-southeast directed extension and with the sedimentary rocks, exist on the continents and appear to be present in the offshore under the salt. We know the paleolows were depositional pathways to funnel sediments from onshore to offshore via water and wind in Jurassic and maybe Cretaceous times. Many tectonic models call for the paleohighs and paleolows to be structurally controlled; however, finding the faults called upon to control the "horst and graben" structures is challenging. We present data from several seismic studies that questions the idea that these paleohighs and paleolows are the result of horst and graben extension. Half grabens exist in the offshore with graben bounding faults northeast-southwest; however, down is to the north instead of the anticipated down to the south. Instead, the basement paleohighs and paleolows in the offshore Eastern Gulf of Mexico may be the result of

Development of an arcuate fold-thrust belt as a result of basement configuration: an example from the Rocky Mountain Front Range, Montana

NASA Astrophysics Data System (ADS)

Burberry, C. M.; Cannon, D. L.; Engelder, T.; Cosgrove, J. W.

2010-12-01

The Sawtooth Range forms part of the Montana Disturbed Belt in the Front Ranges of the Rocky Mountains, along strike from the Alberta Syncline in the Canadian Rockies. The belt developed in the footwall to the Lewis Thrust during the Sevier orogeny and is similar in deformation style to the Canadian Foothills, with a series of stacked thrust sheets carrying Palaeozoic carbonates. The Sawtooth Range can be divided into an inner and outer deformed belt, separated by exposed fold structures in the overlying clastic sequence. Structures in the deformed belts plunge into the culmination of the NE-trending Scapegoat-Bannatyne trend, part of the Great Falls Tectonic Zone (GFTZ). Other mapped faults, including the Pendroy fault zone to the north, parallel this trend. A number of mechanisms have been proposed for the development of primary arcs in fold-thrust belts, including linkage of two thrust belts with different strikes, differential transport of segments of the belt, the geometry of the indentor, local plate heterogeneity and pre-existing basement configuration. Arcuate belts may also develop as a result of later bending of an initially straight orogen. In the Swift Dam area, part of the outer belt of the Sawtooth Range, the strike of the belt changes from 165 to 150. This apparent change in strike is accommodated by a sinistral lateral ramp in the Swift Dam Thrust. In addition, this outer belt becomes broader to the north in the Swift Dam region. However, the outer belt becomes extremely narrow in the Teton Canyon region to the south, and the deformation front is characterised by an intercutaneous wedge structure, rather than the trailing-edge imbricate fan seen to the north. A similar imbricate fan structure is seen to the south, in the Sun River Canyon region, corresponding well to the classic model of a deformation belt governed by a dominant thrust sheet, after Boyer & Elliot. The Sawtooth Range can be described as an active-roof duplex in the footwall to the

Hydrothermal Circulation Within and Between Basement Outcrops on a Young Ridge Flank: Numerical Models and Thermal Constraints

NASA Astrophysics Data System (ADS)

Hutnak, M.; Fisher, A. T.; Stauffer, P.; Gable, C. W.

2005-12-01

We use two-dimensional, finite-element models of coupled heat and fluid flow to investigate local and large-scale heat and fluid transport around and between basement outcrops on a young ridge flank. System geometries and properties are based on observations and measurements on the 3.4-3.6 Ma eastern flank of the Juan de Fuca Ridge. A small area of basement exposure (Baby Bare outcrop) experiences focused hydrothermal discharge, whereas a much larger feature (Grizzly Bare outcrop) 50 km to the south is a site of hydrothermal recharge. Observations of seafloor heat flow, subseafloor pressures, and basement fluid geochemistry at and near these outcrops constrain acceptable model results. Single-outcrop simulations suggest that local convection alone (represented by a high Nusselt number proxy) cannot explain the near-outcrop heat flow patterns; rapid through-flow is required. Venting of at least 5 L/s through the smaller outcrop, a volumetric flow rate consistent with earlier estimates based on plume and outcrop measurements, is needed to match seafloor heat flow patterns. Heat flow patterns are more variable and complex near the larger, recharging outcrop. Simulations that include 5-20 L/s of recharge through this feature can replicate first-order trends in the data, but small-scale variations are likely to result from heterogeneous flow paths and vigorous, local convection. Two-outcrop simulations started with a warm hydrostatic initial condition, based on a conductive model, result in rapid fluid flow from the smaller outcrop to the larger outcrop, inconsistent with observations. Flow can be sustained in the opposite (correct) direction if it is initially forced, which generates a hydrothermal siphon between the two features. Free flow simulations maintain rapid circulation at rates consistent with observations (specific discharge of m/yr to tens of m/yr), provided basement permeability is on the order of 10-10 m2 or greater. Lateral flow rates scale inversely

Distribution of type IV collagen in pancreatic adenocarcinoma and chronic pancreatitis.

PubMed Central

Lee, C. S.; Montebello, J.; Georgiou, T.; Rode, J.

1994-01-01

Changes in the basement membrane are present in various neoplastic conditions such as neurofibrosarcoma, cervical carcinoma, colorectal cancers and hepatoblastoma. This study examines the expression of type IV collagen in the basement membrane, using an immunohistochemical method, in the normal pancreas (n = 10), chronic pancreatitis (n = 15) and pancreatic adenocarcinoma (n = 30). The formalin fixed, paraffin embedded tissue was sectioned and pretreated with protease prior to immunostaining for type IV collagen. There was a statistically significant difference in type IV collagen expression between pancreatic carcinoma and chronic pancreatitis (P = 0.0001; chi 2 test with continuity correction). In pancreatic adenocarcinoma, type IV collagen distribution in the basement membrane was discontinuous and irregular or absent around individual or groups of neoplastic cells (n = 30). Most cases of chronic pancreatitis showed continuous pattern of basement membrane type IV collagen around residual ducts (n = 10). In the normal pancreas, only one of the ten cases showed discontinuous basement membrane around pancreatic ducts, while in the rest of the cases, the pattern was continuous. This study suggests that there is abnormal distribution of type IV collagen in the basement membrane in pancreatic carcinoma, which may be related to either abnormal deposition or degradation of the collagen. Immunostaining for type IV collagen may be of some diagnostic use for distinguishing pancreatic adenocarcinoma from problematic cases of chronic pancreatitis. Images Figure 1 Figure 2 Figure 3 PMID:8199008

Biomimetic, ultrathin and elastic hydrogels regulate human neutrophil extravasation across endothelial-pericyte bilayers.

PubMed

Lauridsen, Holly M; Gonzalez, Anjelica L

2017-01-01

The vascular basement membrane-a thin, elastic layer of extracellular matrix separating and encasing vascular cells-provides biological and mechanical cues to endothelial cells, pericytes, and migrating leukocytes. In contrast, experimental scaffolds typically used to replicate basement membranes are stiff and bio-inert. Here, we present thin, porated polyethylene glycol hydrogels to replicate human vascular basement membranes. Like commercial transwells, our hydrogels are approximately 10μm thick, but like basement membranes, the hydrogels presented here are elastic (E: 50-80kPa) and contain a dense network of small pores. Moreover, the inclusion of bioactive domains introduces receptor-mediated biochemical signaling. We compare elastic hydrogels to common culture substrates (E: >2GPa) for human endothelial cell and pericyte monolayers and bilayers to replicate postcapillary venules in vitro. Our data demonstrate that substrate elasticity facilitates differences in vascular phenotype, supporting expression of vascular markers that are increasingly replicative of venules. Endothelial cells differentially express vascular markers, like EphB4, and leukocyte adhesion molecules, such as ICAM-1, with decreased mechanical stiffness. With porated PEG hydrogels we demonstrate the ability to evaluate and observe leukocyte recruitment across endothelial cell and pericyte monolayers and bilayers, reporting that basement membrane scaffolds can significantly alter the rate of vascular migration in experimental systems. Overall, this study demonstrates the creation and utility of a new and accessible method to recapture the mechanical and biological complexity of human basement membranes in vitro.

Role of the Precambrian Mughese Shear Zone on Cenozoic faulting along the Rukwa-Malawi Rift segment of the East African Rift System

NASA Astrophysics Data System (ADS)

Heilman, E.; Kolawole, F.; Mayle, M.; Atekwana, E. A.; Abdelsalam, M. G.

2017-12-01

We address the longstanding question of the role of long-lived basement structures in strain accommodation within active rift systems. Studies have highlighted the influence of pre-existing zones of lithospheric weakness in modulating faulting and fault kinematics. Here, we investigate the role of the Neoproterozoic Mughese Shear Zone (MSZ) in Cenozoic rifting along the Rukwa-Malawi rift segment of the East African Rift System (EARS). Detailed analyses of Shuttle Radar Topography Mission (SRTM) DEM and filtered aeromagnetic data allowed us to determine the relationship between rift-related basement-rooted normal faults and the MSZ fabric extending along the southern boundary of the Rukwa-Malawi Rift North Basin. Our results show that the magnetic lineaments defining the MSZ coincide with the collinear Rukwa Rift border fault (Ufipa Fault), a dextral strike-slip fault (Mughese Fault), and the North Basin hinge-zone fault (Mbiri Fault). Fault-scarp and minimum fault-throw analyses reveal that within the Rukwa Rift, the Ufipa Border Fault has been accommodating significant displacement relative to the Lupa Border Fault, which represents the northeastern border fault of the Rukwa Rift. Our analysis also shows that within the North Basin half-graben, the Mbiri Fault has accommodated the most vertical displacement relative to other faults along the half-graben hinge zone. We propose that the Cenozoic reactivation along the MSZ facilitated significant normal slip displacement along the Ufipa Border Fault and the Mbiri Fault, and minor dextral strike-slip between the two faults. We suggest that the fault kinematics along the Rukwa-Malawi Rift is the result of reactivation of the MSZ through regional oblique extension.

Volume gain during shearing of the Whatley Mill Gneiss, Pine Mountain Basement massif, eastern Alabama--A trace element approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Salpas, P.A.; Daniell, N.

1993-03-01

The Whatley Mill Gneiss is the most voluminous exposure of the Pine Mountain Basement massif in eastern Alabama. Its type lithology is a proto-mylonitic gneiss composed of K-spar augen, up to 5 cm in diameter, in a finer matrix of biotite, microcline, and quartz. Granulite-facies mineral assemblages in the Whatley Mill Gneiss have been completely retrograded to amphibolite- and greenschist-facies assemblages in response to deformation that produced shear zones paralleling the foliation of the gneiss. The augen gneiss and its associated mylonites are well-exposed in a creek bed in Chewacla State Park. At this location the mineralogy of the mylonitesmore » is dominated by quartz indicating that shearing was associated with influx of a silica-rich fluid. A detailed geochemical study of these rocks shows that the augen gneiss displays relatively little variation in its major and trace element compositions while the quartz-rich mylonites display wider ranges, are enriched in SiO[sub 2] and depleted in the REE and other incompatible trace elements relative to the augen gneiss. When standard composition/volume calculations are applied to the mylonites the results show (1) the bulk of all of the elements, including the REE, were immobile during shearing with the exceptions of Si and Al which were added; and, (2) volume changes calculated using the REE as immobile elements range from +70% to +350%. Though these volume changes seem excessive, they apply to meter-thick shear zones which may actually represent only a small fraction of the total volume of the augen gneiss. Consistent with previous interpretations of these shear zones, the calculated volume gains imply shearing during extension.« less

4. INTERIOR VIEW OF BASEMENT. CENTRAL LINE SHAFTING RUNNING NORTHSOUTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. INTERIOR VIEW OF BASEMENT. CENTRAL LINE SHAFTING RUNNING NORTH-SOUTH IS IN PLACE; AT RIGHT IS A PRESS FOR WORKING THE ALUMINUM SHEETS; E. W. BLISS CO. OF BROOKLYN, NEW YORK, MANUFACTURED THE PRESS. MACHINERY ORIGINALLY POWERED BY OVERHEAD BELTS CONNECTED TO CENTRAL LINE SHAFTS; BY ABOUT THE 1940s THE MACHINERY WAS ELECTRICALLY POWERED. - Illinois Pure Aluminum Company, 109 Holmes Street, Lemont, Cook County, IL

Microbial diversity within Juan de Fuca ridge basement fluids sampled from oceanic borehole observatories

NASA Astrophysics Data System (ADS)