Satellite Doppler data processing using a microcomputer

NASA Technical Reports Server (NTRS)

Schmid, P. E.; Lynn, J. J.

1977-01-01

A microcomputer which was developed to compute ground radio beacon position locations using satellite measurements of Doppler frequency shift is described. Both the computational algorithms and the microcomputer hardware incorporating these algorithms were discussed. Results are presented where the microcomputer in conjunction with the NIMBUS-6 random access measurement system provides real time calculation of beacon latitude and longitude.

Development of satellite position location system for aircraft and boat distress beacons

NASA Technical Reports Server (NTRS)

Kahle, D. R.

1982-01-01

An international satellite system for the detection and location of distress beacons carried on aircraft and vessels is in its technical checkout phase. User demonstration and evaluation (D&E) will start in early 1983 and continue for two years. The D&E phase and a subsequent transition period will form the basis for improved search and rescue operations for the 1980's and beyond. The system, called COSPAS/SARSAT, has international participation involving the U.S., Canada, and France as SARSAT members and joint participation with the Soviet Union's COSPAS Project. Norway and the U.K. have recently joined as investigators. Average position location error of the satellite aided processing is expected to be from 10-20KM for the existing 121.5/243 MHz distress beacons and from 2-5KM for experimental beacons transmitting in the 406 MHz band.

Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons.

PubMed

Weusten, Jos J A M; Carpay, Wim M; Oosterlaken, Tom A M; van Zuijlen, Martien C A; van de Wiel, Paul A

2002-03-15

For quantitative NASBA-based viral load assays using homogeneous detection with molecular beacons, such as the NucliSens EasyQ HIV-1 assay, a quantitation algorithm is required. During the amplification process there is a constant growth in the concentration of amplicons to which the beacon can bind while generating a fluorescence signal. The overall fluorescence curve contains kinetic information on both amplicon formation and beacon binding, but only the former is relevant for quantitation. In the current paper, mathematical modeling of the relevant processes is used to develop an equation describing the fluorescence curve as a function of the amplification time and the relevant kinetic parameters. This equation allows reconstruction of RNA formation, which is characterized by an exponential increase in concentrations as long as the primer concentrations are not rate limiting and by linear growth over time after the primer pool is depleted. During the linear growth phase, the actual quantitation is based on assessing the amplicon formation rate from the viral RNA relative to that from a fixed amount of calibrator RNA. The quantitation procedure has been successfully applied in the NucliSens EasyQ HIV-1 assay.

Caged molecular beacons: controlling nucleic acid hybridization with light.

PubMed

Wang, Chunming; Zhu, Zhi; Song, Yanling; Lin, Hui; Yang, Chaoyong James; Tan, Weihong

2011-05-28

We have constructed a novel class of light-activatable caged molecular beacons (cMBs) that are caged by locking two stems with a photo-labile biomolecular interaction or covalent bond. With the cMBs, the nucleic acid hybridization process can be easily controlled with light, which offers the possibility for a high spatiotemporal resolution study of intracellular mRNAs. © The Royal Society of Chemistry 2011

Novel approach for beacon formation through simulated turbulence: initial lab-test results

NASA Astrophysics Data System (ADS)

Khizhnyak, A.; Markov, V.; Tomov, I.; Wu, F.

2010-02-01

In this paper we report the results of the analysis and experimental modeling of the target-in-the-loop (TIL) approach that is used to form a localized beacon for a laser beam propagating through turbulent atmosphere. The analogy between the TIL system and the laser cavity has been used here to simulate the process shaping the laser beacon on a remote image-resolved target with rough surface. The TIL breadboard was integrated and used for laboratory modeling of the proposed approach. This breadboard allowed to simulate the TIL arrangement with a rough-surface target and laser beam propagation through the turbulent atmospheric layer. Here we present the initial results of the performed studies.

Gold nanoparticle-based beacon to detect STAT5b mRNA expression in living cells: a case optimized by bioinformatics screen.

PubMed

Deng, Dawei; Li, Yang; Xue, Jianpeng; Wang, Jie; Ai, Guanhua; Li, Xin; Gu, Yueqing

2015-01-01

Messenger RNA (mRNA), a single-strand ribonucleic acid with functional gene information is usually abnormally expressed in cancer cells and has become a promising biomarker for the study of tumor progress. Hairpin DNA-coated gold nanoparticle (hDAuNP) beacon containing a bare gold nanoparticle (AuNP) as fluorescence quencher and thiol-terminated fluorescently labeled stem-loop-stem oligonucleotide sequences attached by Au-S bond is currently a new nanoscale biodiagnostic platform capable of mRNA detection, in which the design of the loop region sequence is crucial for hybridizing with the target mRNA. Hence, in this study, to improve the sensitivity and selectivity of hDAuNP beacon simultaneously, the loop region of hairpin DNA was screened by bioinformatics strategy. Here, signal transducer and activator of transcription 5b (STAT5b) mRNA was selected and used as a practical example. The results from the combined characterizations using optical techniques, flow cytometry assay, and cell microscopic imaging showed that after optimization, the as-prepared hDAuNP beacon had higher selectivity and sensitivity for the detection of STAT5b mRNA in living cells, as compared with our previous beacon. Thus, the bioinformatics method may be a promising new strategy for assisting in the designing of the hDAuNP beacon, extending its application in the detection of mRNA expression and the resultant mRNA-based biological processes and disease pathogenesis.

Dual Functional Roles of Molecular Beacon as a MicroRNA Detector and Inhibitor*

PubMed Central

Li, Wai Ming; Chan, Ching-Man; Miller, Andrew L.; Lee, Chow H.

2017-01-01

MicroRNAs are essential in many cellular processes. The ability to detect microRNAs is important for understanding its function and biogenesis. This study is aimed at using a molecular beacon to detect miR-430 in developing zebrafish embryos as a proof of principle. miR-430 is crucial for the clearance of maternal mRNA during maternal zygotic transition in embryonic development. Despite its known function, the temporal and spatial expression of miR-430 remains unclear. We used various imaging techniques, including laser scanning confocal microscopy, spinning disk, and lightsheet microscopy, to study the localization of miR-430 and any developmental defects possibly caused by the molecular beacon. Our results show that miR-430 is expressed early in development and is localized in distinct cytoplasmic granules where its target mRNA can be detected. We also show that the designed molecular beacon can inhibit the function of miR-430 and cause developmental defect in the brain, notochord, heart, and kidney, depending on the delivery site within the embryo, suggesting that miR-430 plays a diverse role in embryonic morphogenesis. When compared with morpholino, molecular beacon is 2 orders of magnitude more potent in inhibiting miR-430. Thus, our results reveal that in addition to being used as a valuable tool for the detection of microRNAs in vivo, molecular beacons can also be employed to inhibit microRNAs in a specific manner. PMID:28100783

Gold nanoparticle-based beacon to detect STAT5b mRNA expression in living cells: a case optimized by bioinformatics screen

PubMed Central

Deng, Dawei; Li, Yang; Xue, Jianpeng; Wang, Jie; Ai, Guanhua; Li, Xin; Gu, Yueqing

2015-01-01

Messenger RNA (mRNA), a single-strand ribonucleic acid with functional gene information is usually abnormally expressed in cancer cells and has become a promising biomarker for the study of tumor progress. Hairpin DNA-coated gold nanoparticle (hDAuNP) beacon containing a bare gold nanoparticle (AuNP) as fluorescence quencher and thiol-terminated fluorescently labeled stem–loop–stem oligonucleotide sequences attached by Au–S bond is currently a new nanoscale biodiagnostic platform capable of mRNA detection, in which the design of the loop region sequence is crucial for hybridizing with the target mRNA. Hence, in this study, to improve the sensitivity and selectivity of hDAuNP beacon simultaneously, the loop region of hairpin DNA was screened by bioinformatics strategy. Here, signal transducer and activator of transcription 5b (STAT5b) mRNA was selected and used as a practical example. The results from the combined characterizations using optical techniques, flow cytometry assay, and cell microscopic imaging showed that after optimization, the as-prepared hDAuNP beacon had higher selectivity and sensitivity for the detection of STAT5b mRNA in living cells, as compared with our previous beacon. Thus, the bioinformatics method may be a promising new strategy for assisting in the designing of the hDAuNP beacon, extending its application in the detection of mRNA expression and the resultant mRNA-based biological processes and disease pathogenesis. PMID:25987838

An Autonomous Distributed Fault-Tolerant Local Positioning System

NASA Technical Reports Server (NTRS)

Malekpour, Mahyar R.

2017-01-01

We describe a fault-tolerant, GPS-independent (Global Positioning System) distributed autonomous positioning system for static/mobile objects and present solutions for providing highly-accurate geo-location data for the static/mobile objects in dynamic environments. The reliability and accuracy of a positioning system fundamentally depends on two factors; its timeliness in broadcasting signals and the knowledge of its geometry, i.e., locations and distances of the beacons. Existing distributed positioning systems either synchronize to a common external source like GPS or establish their own time synchrony using a scheme similar to a master-slave by designating a particular beacon as the master and other beacons synchronize to it, resulting in a single point of failure. Another drawback of existing positioning systems is their lack of addressing various fault manifestations, in particular, communication link failures, which, as in wireless networks, are increasingly dominating the process failures and are typically transient and mobile, in the sense that they typically affect different messages to/from different processes over time.

Crew Activity Analyzer

NASA Technical Reports Server (NTRS)

Murray, James; Kirillov, Alexander

2008-01-01

The crew activity analyzer (CAA) is a system of electronic hardware and software for automatically identifying patterns of group activity among crew members working together in an office, cockpit, workshop, laboratory, or other enclosed space. The CAA synchronously records multiple streams of data from digital video cameras, wireless microphones, and position sensors, then plays back and processes the data to identify activity patterns specified by human analysts. The processing greatly reduces the amount of time that the analysts must spend in examining large amounts of data, enabling the analysts to concentrate on subsets of data that represent activities of interest. The CAA has potential for use in a variety of governmental and commercial applications, including planning for crews for future long space flights, designing facilities wherein humans must work in proximity for long times, improving crew training and measuring crew performance in military settings, human-factors and safety assessment, development of team procedures, and behavioral and ethnographic research. The data-acquisition hardware of the CAA (see figure) includes two video cameras: an overhead one aimed upward at a paraboloidal mirror on the ceiling and one mounted on a wall aimed in a downward slant toward the crew area. As many as four wireless microphones can be worn by crew members. The audio signals received from the microphones are digitized, then compressed in preparation for storage. Approximate locations of as many as four crew members are measured by use of a Cricket indoor location system. [The Cricket indoor location system includes ultrasonic/radio beacon and listener units. A Cricket beacon (in this case, worn by a crew member) simultaneously transmits a pulse of ultrasound and a radio signal that contains identifying information. Each Cricket listener unit measures the difference between the times of reception of the ultrasound and radio signals from an identified beacon. Assuming essentially instantaneous propagation of the radio signal, the distance between that beacon and the listener unit is estimated from this time difference and the speed of sound in air.] In this system, six Cricket listener units are mounted in various positions on the ceiling, and as many as four Cricket beacons are attached to crew members. The three-dimensional position of each Cricket beacon can be estimated from the time-difference readings of that beacon from at least three Cricket listener units

Action Research on Development and Application of Internet of Things Services in Hospital.

PubMed

Park, Arum; Chang, Hyejung; Lee, Kyoung Jun

2017-01-01

Services based on the Internet of Things (IoT) technologies have emerged in various business environments. To enhance health service quality and maximize benefits, this study applied an IoT technology based on NFC and iBeacon as an omni-channel service for patient care in hospitals. Application of the IoT technology based on NFC and iBeacon was conducted in a general hospital during August 2015 through June 2016, and the development and evaluation results were aligned to an action research framework. The five phases in the action research included diagnosing, planning action, taking action, evaluating action, and specifying learning phases. During the first two phases, problems of functional operations in a hospital were diagnosed and eight service models were designed by using iBeacon and NFC to solve the problems. Service models were applied to the hospital by installing beacons, wearable beacons, beacon scanners, and NFC tags during the third phase. During the fourth and fifth phases, the roles and benefits of stakeholders participating in the service models were evaluated, and issues and knowledge of the whole application process were derived and summarized from technological, economic, social and legal perspectives, respectively. From an action research perspective, IoT-based healthcare services were developed and verified. IoT-based services enable the hospital to acquire lifelog data for precision medicine and ultimately be able to go one step closer to precision medical care. The derived service models could provide patients more enhanced healthcare services and improve the work efficiency and effectiveness of the hospital.

Action Research on Development and Application of Internet of Things Services in Hospital

PubMed Central

Park, Arum; Chang, Hyejung

2017-01-01

Objectives Services based on the Internet of Things (IoT) technologies have emerged in various business environments. To enhance health service quality and maximize benefits, this study applied an IoT technology based on NFC and iBeacon as an omni-channel service for patient care in hospitals. Methods Application of the IoT technology based on NFC and iBeacon was conducted in a general hospital during August 2015 through June 2016, and the development and evaluation results were aligned to an action research framework. The five phases in the action research included diagnosing, planning action, taking action, evaluating action, and specifying learning phases. Results During the first two phases, problems of functional operations in a hospital were diagnosed and eight service models were designed by using iBeacon and NFC to solve the problems. Service models were applied to the hospital by installing beacons, wearable beacons, beacon scanners, and NFC tags during the third phase. During the fourth and fifth phases, the roles and benefits of stakeholders participating in the service models were evaluated, and issues and knowledge of the whole application process were derived and summarized from technological, economic, social and legal perspectives, respectively. Conclusions From an action research perspective, IoT-based healthcare services were developed and verified. IoT-based services enable the hospital to acquire lifelog data for precision medicine and ultimately be able to go one step closer to precision medical care. The derived service models could provide patients more enhanced healthcare services and improve the work efficiency and effectiveness of the hospital. PMID:28261528

Dual Functional Roles of Molecular Beacon as a MicroRNA Detector and Inhibitor.

PubMed

Li, Wai Ming; Chan, Ching-Man; Miller, Andrew L; Lee, Chow H

2017-03-03

MicroRNAs are essential in many cellular processes. The ability to detect microRNAs is important for understanding its function and biogenesis. This study is aimed at using a molecular beacon to detect miR-430 in developing zebrafish embryos as a proof of principle. miR-430 is crucial for the clearance of maternal mRNA during maternal zygotic transition in embryonic development. Despite its known function, the temporal and spatial expression of miR-430 remains unclear. We used various imaging techniques, including laser scanning confocal microscopy, spinning disk, and lightsheet microscopy, to study the localization of miR-430 and any developmental defects possibly caused by the molecular beacon. Our results show that miR-430 is expressed early in development and is localized in distinct cytoplasmic granules where its target mRNA can be detected. We also show that the designed molecular beacon can inhibit the function of miR-430 and cause developmental defect in the brain, notochord, heart, and kidney, depending on the delivery site within the embryo, suggesting that miR-430 plays a diverse role in embryonic morphogenesis. When compared with morpholino, molecular beacon is 2 orders of magnitude more potent in inhibiting miR-430. Thus, our results reveal that in addition to being used as a valuable tool for the detection of microRNAs in vivo , molecular beacons can also be employed to inhibit microRNAs in a specific manner. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Digital Controller For Emergency Beacon

NASA Technical Reports Server (NTRS)

Ivancic, William D.

1990-01-01

Prototype digital controller intended for use in 406-MHz emergency beacon. Undergoing development according to international specifications, 406-MHz emergency beacon system includes satellites providing worldwide monitoring of beacons, with Doppler tracking to locate each beacon within 5 km. Controller turns beacon on and off and generates binary codes identifying source (e.g., ship, aircraft, person, or vehicle on land). Codes transmitted by phase modulation. Knowing code, monitor attempts to communicate with user, monitor uses code information to dispatch rescue team appropriate to type and locations of carrier.

Smartphone-Based Indoor Localization with Bluetooth Low Energy Beacons

PubMed Central

Zhuang, Yuan; Yang, Jun; Li, You; Qi, Longning; El-Sheimy, Naser

2016-01-01

Indoor wireless localization using Bluetooth Low Energy (BLE) beacons has attracted considerable attention after the release of the BLE protocol. In this paper, we propose an algorithm that uses the combination of channel-separate polynomial regression model (PRM), channel-separate fingerprinting (FP), outlier detection and extended Kalman filtering (EKF) for smartphone-based indoor localization with BLE beacons. The proposed algorithm uses FP and PRM to estimate the target’s location and the distances between the target and BLE beacons respectively. We compare the performance of distance estimation that uses separate PRM for three advertisement channels (i.e., the separate strategy) with that use an aggregate PRM generated through the combination of information from all channels (i.e., the aggregate strategy). The performance of FP-based location estimation results of the separate strategy and the aggregate strategy are also compared. It was found that the separate strategy can provide higher accuracy; thus, it is preferred to adopt PRM and FP for each BLE advertisement channel separately. Furthermore, to enhance the robustness of the algorithm, a two-level outlier detection mechanism is designed. Distance and location estimates obtained from PRM and FP are passed to the first outlier detection to generate improved distance estimates for the EKF. After the EKF process, the second outlier detection algorithm based on statistical testing is further performed to remove the outliers. The proposed algorithm was evaluated by various field experiments. Results show that the proposed algorithm achieved the accuracy of <2.56 m at 90% of the time with dense deployment of BLE beacons (1 beacon per 9 m), which performs 35.82% better than <3.99 m from the Propagation Model (PM) + EKF algorithm and 15.77% more accurate than <3.04 m from the FP + EKF algorithm. With sparse deployment (1 beacon per 18 m), the proposed algorithm achieves the accuracies of <3.88 m at 90% of the time, which performs 49.58% more accurate than <8.00 m from the PM + EKF algorithm and 21.41% better than <4.94 m from the FP + EKF algorithm. Therefore, the proposed algorithm is especially useful to improve the localization accuracy in environments with sparse beacon deployment. PMID:27128917

Smartphone-Based Indoor Localization with Bluetooth Low Energy Beacons.

PubMed

Zhuang, Yuan; Yang, Jun; Li, You; Qi, Longning; El-Sheimy, Naser

2016-04-26

Indoor wireless localization using Bluetooth Low Energy (BLE) beacons has attracted considerable attention after the release of the BLE protocol. In this paper, we propose an algorithm that uses the combination of channel-separate polynomial regression model (PRM), channel-separate fingerprinting (FP), outlier detection and extended Kalman filtering (EKF) for smartphone-based indoor localization with BLE beacons. The proposed algorithm uses FP and PRM to estimate the target's location and the distances between the target and BLE beacons respectively. We compare the performance of distance estimation that uses separate PRM for three advertisement channels (i.e., the separate strategy) with that use an aggregate PRM generated through the combination of information from all channels (i.e., the aggregate strategy). The performance of FP-based location estimation results of the separate strategy and the aggregate strategy are also compared. It was found that the separate strategy can provide higher accuracy; thus, it is preferred to adopt PRM and FP for each BLE advertisement channel separately. Furthermore, to enhance the robustness of the algorithm, a two-level outlier detection mechanism is designed. Distance and location estimates obtained from PRM and FP are passed to the first outlier detection to generate improved distance estimates for the EKF. After the EKF process, the second outlier detection algorithm based on statistical testing is further performed to remove the outliers. The proposed algorithm was evaluated by various field experiments. Results show that the proposed algorithm achieved the accuracy of <2.56 m at 90% of the time with dense deployment of BLE beacons (1 beacon per 9 m), which performs 35.82% better than <3.99 m from the Propagation Model (PM) + EKF algorithm and 15.77% more accurate than <3.04 m from the FP + EKF algorithm. With sparse deployment (1 beacon per 18 m), the proposed algorithm achieves the accuracies of <3.88 m at 90% of the time, which performs 49.58% more accurate than <8.00 m from the PM + EKF algorithm and 21.41% better than <4.94 m from the FP + EKF algorithm. Therefore, the proposed algorithm is especially useful to improve the localization accuracy in environments with sparse beacon deployment.

Tester for Distress Beacons

NASA Technical Reports Server (NTRS)

Wade, W. R.

1986-01-01

Distress beacons on aircraft and boats checked for proper operation with aid of onboard monitor. Monitor mounted in aircraft cockpit or at wheel of boat. Connected to beacon electronics by cable. Monitor used with interface circuitry in beacon, which acts as buffer so that operation of beacon is not adversely affected if monitor is removed or if connecting cable is accidentally short circuited.

Magnetic resonance beacon to detect intracellular microRNA during neurogenesis.

PubMed

Lee, Jonghwan; Jin, Yeon A; Ko, Hae Young; Lee, Yong Seung; Heo, Hyejung; Cho, Sujeong; Kim, Soonhag

2015-02-01

Magnetic resonance imaging (MRI) offers great spatial resolution for viewing deep tissues and anatomy. We developed a self-assembling signal-on magnetic fluorescence nanoparticle to visualize intracellular microRNAs (miRNAs or miRs) during neurogenesis using MRI. The self-assembling nanoparticle (miR124a MR beacon) was aggregated by the incubation of three different oligonucleotides: a 3' adaptor, a 5' adaptor, and a linker containing miR124a-binding sequences. The T2-weighted magnetic resonance (MR) signal of the self-assembled nanoparticle was quenched when miR124a was absent from test tubes or was minimally expressed in cells and tissues. When miR124a was present in test tubes or highly expressed in vitro and in vivo during P19 cell neurogenesis, it hybridized with the miR124a MR beacon, causing the linker to detach, resulting in increased signal-on MRI intensity. This MR beacon can be used as a new imaging probe to monitor the miRNA-mediated regulation of cellular processes. Copyright © 2014 Elsevier Ltd. All rights reserved.

Robot-Beacon Distributed Range-Only SLAM for Resource-Constrained Operation

PubMed Central

Torres-González, Arturo; Martínez-de Dios, Jose Ramiro; Ollero, Anibal

2017-01-01

This work deals with robot-sensor network cooperation where sensor nodes (beacons) are used as landmarks for Range-Only (RO) Simultaneous Localization and Mapping (SLAM). Most existing RO-SLAM techniques consider beacons as passive devices disregarding the sensing, computational and communication capabilities with which they are actually endowed. SLAM is a resource-demanding task. Besides the technological constraints of the robot and beacons, many applications impose further resource consumption limitations. This paper presents a scalable distributed RO-SLAM scheme for resource-constrained operation. It is capable of exploiting robot-beacon cooperation in order to improve SLAM accuracy while meeting a given resource consumption bound expressed as the maximum number of measurements that are integrated in SLAM per iteration. The proposed scheme combines a Sparse Extended Information Filter (SEIF) SLAM method, in which each beacon gathers and integrates robot-beacon and inter-beacon measurements, and a distributed information-driven measurement allocation tool that dynamically selects the measurements that are integrated in SLAM, balancing uncertainty improvement and resource consumption. The scheme adopts a robot-beacon distributed approach in which each beacon participates in the selection, gathering and integration in SLAM of robot-beacon and inter-beacon measurements, resulting in significant estimation accuracies, resource-consumption efficiency and scalability. It has been integrated in an octorotor Unmanned Aerial System (UAS) and evaluated in 3D SLAM outdoor experiments. The experimental results obtained show its performance and robustness and evidence its advantages over existing methods. PMID:28425946

Robot-Beacon Distributed Range-Only SLAM for Resource-Constrained Operation.

PubMed

Torres-González, Arturo; Martínez-de Dios, Jose Ramiro; Ollero, Anibal

2017-04-20

This work deals with robot-sensor network cooperation where sensor nodes (beacons) are used as landmarks for Range-Only (RO) Simultaneous Localization and Mapping (SLAM). Most existing RO-SLAM techniques consider beacons as passive devices disregarding the sensing, computational and communication capabilities with which they are actually endowed. SLAM is a resource-demanding task. Besides the technological constraints of the robot and beacons, many applications impose further resource consumption limitations. This paper presents a scalable distributed RO-SLAM scheme for resource-constrained operation. It is capable of exploiting robot-beacon cooperation in order to improve SLAM accuracy while meeting a given resource consumption bound expressed as the maximum number of measurements that are integrated in SLAM per iteration. The proposed scheme combines a Sparse Extended Information Filter (SEIF) SLAM method, in which each beacon gathers and integrates robot-beacon and inter-beacon measurements, and a distributed information-driven measurement allocation tool that dynamically selects the measurements that are integrated in SLAM, balancing uncertainty improvement and resource consumption. The scheme adopts a robot-beacon distributed approach in which each beacon participates in the selection, gathering and integration in SLAM of robot-beacon and inter-beacon measurements, resulting in significant estimation accuracies, resource-consumption efficiency and scalability. It has been integrated in an octorotor Unmanned Aerial System (UAS) and evaluated in 3D SLAM outdoor experiments. The experimental results obtained show its performance and robustness and evidence its advantages over existing methods.

Building and strengthening infrastructure for data exchange: lessons from the beacon communities.

PubMed

Torres, Gretchen W; Swietek, Karen; Ubri, Petry S; Singer, Rachel F; Lowell, Kristina H; Miller, Wilhelmine

2014-01-01

The Beacon Community Cooperative Agreement Program supports interventions, including care-delivery innovations, provider performance measurement and feedback initiatives, and tools for providers and consumers to enhance care. Using a learning health system framework, we examine the Beacon Communities' processes in building and strengthening health IT (HIT) infrastructures, specifically successes and challenges in sharing patient information to improve clinical care. In 2010, the Office of the National Coordinator for Health Information Technology (ONC) launched the three-year program, which provided $250 million to 17 Beacon Communities to invest in HIT and health information exchange (HIE) infrastructure. Beacon Communities used this funding to develop and disseminate HIT-enabled quality improvement practices found effective in particular community and practice environments. NORC conducted 7 site visits, November 2012-March 2013, selecting Communities to represent diverse program features. From August-October 2013, NORC held discussions with the remaining 10 Communities. Following each visit or discussion, NORC summarized the information gathered, including transcripts, team observations, and other documents the Community provided, to facilitate a within-Community analysis of context and stakeholders, intervention strategies, enabling factors, and challenges. Although each Community designed and implemented data-sharing strategies in a unique environment, similar challenges and enabling factors emerged across the Beacons. From a learning health system perspective, their strategies to build and strengthen data-sharing infrastructures address the following crosscutting priorities: promoting technical advances and innovations by helping providers adapt EHRs for data exchange and performance measurement with customizable IT and offering technical support to smaller, independent providers; engaging key stakeholders; and fostering transparent governance and stewardship of the infrastructure with neutral conveners. While all the Communities developed or strengthened data-exchange infrastructure, each did this in a unique environment of existing health care market and legal factors. The Communities, however, encountered similar challenges and enabling factors. Organizations undertaking collaborative data sharing, performance measurement and clinical transformation can learn from the Beacon Communities' experience.

Building and Strengthening Infrastructure for Data Exchange: Lessons from the Beacon Communities

PubMed Central

Torres, Gretchen W.; Swietek, Karen; Ubri, Petry S.; Singer, Rachel F.; Lowell, Kristina H.; Miller, Wilhelmine

2014-01-01

Introduction: The Beacon Community Cooperative Agreement Program supports interventions, including care-delivery innovations, provider performance measurement and feedback initiatives, and tools for providers and consumers to enhance care. Using a learning health system framework, we examine the Beacon Communities’ processes in building and strengthening health IT (HIT) infrastructures, specifically successes and challenges in sharing patient information to improve clinical care. Background: In 2010, the Office of the National Coordinator for Health Information Technology (ONC) launched the three-year program, which provided $250 million to 17 Beacon Communities to invest in HIT and health information exchange (HIE) infrastructure. Beacon Communities used this funding to develop and disseminate HIT-enabled quality improvement practices found effective in particular community and practice environments. Methods: NORC conducted 7 site visits, November 2012–March 2013, selecting Communities to represent diverse program features. From August–October 2013, NORC held discussions with the remaining 10 Communities. Following each visit or discussion, NORC summarized the information gathered, including transcripts, team observations, and other documents the Community provided, to facilitate a within-Community analysis of context and stakeholders, intervention strategies, enabling factors, and challenges. Results: Although each Community designed and implemented data-sharing strategies in a unique environment, similar challenges and enabling factors emerged across the Beacons. From a learning health system perspective, their strategies to build and strengthen data-sharing infrastructures address the following crosscutting priorities: promoting technical advances and innovations by helping providers adapt EHRs for data exchange and performance measurement with customizable IT and offering technical support to smaller, independent providers; engaging key stakeholders; and fostering transparent governance and stewardship of the infrastructure with neutral conveners. Conclusion: While all the Communities developed or strengthened data-exchange infrastructure, each did this in a unique environment of existing health care market and legal factors. The Communities, however, encountered similar challenges and enabling factors. Organizations undertaking collaborative data sharing, performance measurement and clinical transformation can learn from the Beacon Communities’ experience. PMID:25848619

SU-E-J-257: Image Artifacts Caused by Implanted Calypso Beacons in MRI Studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Amro, H; Chetty, I; Gordon, J

2014-06-01

Purpose: The presence of Calypso Beacon-transponders in patients can cause artifacts during MRI imaging studies. This could be a problem for post-treatment follow up of cancer patients using MRI studies to evaluate metastasis and for functional imaging studies.This work assesses (1) the volume immediately surrounding the transponders that will not be visualized by the MRI due to the beacons, and (2) the dependence of the non-visualized volume on beacon orientation, and scanning techniques. Methods: Two phantoms were used in this study (1) water filled box, (2) and a 2300 cc block of pork meat. Calypso beacons were implanted in themore » phantoms both in parallel and perpendicular orientations with respect to the MR scanner magnetic field. MR image series of the phantom were obtained with on a 1.0T high field open MR-SIM with multiple pulse sequences, for example, T1-weighted fast field echo and T2-weighted turbo spin echo. Results: On average, a no-signal region with 2 cm radius and 3 cm length was measured. Image artifacts are more significant when beacons are placed parallel to scanner magnetic field; the no-signal area around the beacon was about 0.5 cm larger in orthogonal orientation. The no-signal region surrounding the beacons slightly varies in dimension for the different pulse sequences. Conclusion: The use of Calypso beacons can prohibit the use of MRI studies in post-treatment assessments, especially in the immediate region surrounding the implanted beacon. A characterization of the MR scanner by identifying the no-signal regions due to implanted beacons is essential. This may render the use of Calypso beacons useful for some cases and give the treating physician a chance to identify those patients prior to beacon implantation.« less

Privacy Risks from Genomic Data-Sharing Beacons

PubMed Central

Shringarpure, Suyash S.; Bustamante, Carlos D.

2015-01-01

The human genetics community needs robust protocols that enable secure sharing of genomic data from participants in genetic research. Beacons are web servers that answer allele-presence queries—such as “Do you have a genome that has a specific nucleotide (e.g., A) at a specific genomic position (e.g., position 11,272 on chromosome 1)?”—with either “yes” or “no.” Here, we show that individuals in a beacon are susceptible to re-identification even if the only data shared include presence or absence information about alleles in a beacon. Specifically, we propose a likelihood-ratio test of whether a given individual is present in a given genetic beacon. Our test is not dependent on allele frequencies and is the most powerful test for a specified false-positive rate. Through simulations, we showed that in a beacon with 1,000 individuals, re-identification is possible with just 5,000 queries. Relatives can also be identified in the beacon. Re-identification is possible even in the presence of sequencing errors and variant-calling differences. In a beacon constructed with 65 European individuals from the 1000 Genomes Project, we demonstrated that it is possible to detect membership in the beacon with just 250 SNPs. With just 1,000 SNP queries, we were able to detect the presence of an individual genome from the Personal Genome Project in an existing beacon. Our results show that beacons can disclose membership and implied phenotypic information about participants and do not protect privacy a priori. We discuss risk mitigation through policies and standards such as not allowing anonymous pings of genetic beacons and requiring minimum beacon sizes. PMID:26522470

Privacy Risks from Genomic Data-Sharing Beacons.

PubMed

Shringarpure, Suyash S; Bustamante, Carlos D

2015-11-05

The human genetics community needs robust protocols that enable secure sharing of genomic data from participants in genetic research. Beacons are web servers that answer allele-presence queries--such as "Do you have a genome that has a specific nucleotide (e.g., A) at a specific genomic position (e.g., position 11,272 on chromosome 1)?"--with either "yes" or "no." Here, we show that individuals in a beacon are susceptible to re-identification even if the only data shared include presence or absence information about alleles in a beacon. Specifically, we propose a likelihood-ratio test of whether a given individual is present in a given genetic beacon. Our test is not dependent on allele frequencies and is the most powerful test for a specified false-positive rate. Through simulations, we showed that in a beacon with 1,000 individuals, re-identification is possible with just 5,000 queries. Relatives can also be identified in the beacon. Re-identification is possible even in the presence of sequencing errors and variant-calling differences. In a beacon constructed with 65 European individuals from the 1000 Genomes Project, we demonstrated that it is possible to detect membership in the beacon with just 250 SNPs. With just 1,000 SNP queries, we were able to detect the presence of an individual genome from the Personal Genome Project in an existing beacon. Our results show that beacons can disclose membership and implied phenotypic information about participants and do not protect privacy a priori. We discuss risk mitigation through policies and standards such as not allowing anonymous pings of genetic beacons and requiring minimum beacon sizes. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Evaluation of Pedestrian Hybrid Beacons and Rapid Flashing Beacons

DOT National Transportation Integrated Search

2016-07-01

Two pedestrian treatments receiving national attention are the rectangular rapid-flashing beacon (RRFB) and the pedestrian hybrid beacon (PHB). These devices have unique characteristics that produce improved vehicle stopping and yielding to crossing ...

Evaluation of Skylab earth laser beacon imagery. [spaceborne photography

NASA Technical Reports Server (NTRS)

Piech, K. R.; Schott, J. R.

1975-01-01

During the Skylab 3 and 4 missions the Skylab spacecraft was illuminated by a low power argon ion and dye laser. The earth laser beacon was studied visually by the astronauts. In addition, they collected 35 mm hand-held color photographs of the beacons. Photographs are shown that were obtained on Skylab 3 and Skylab 4. The imagery collected during the Skylab mission was analyzed to evaluate the utility of beacon lasers as terrestial 'artificial stars' for space navigation. The analyses of the imagery revealed two unusual features of the earth laser beacon: (1) The beacon, even though of a low power (approximately 1 watt), is considerably brighter than any other terrain feature and is readily visible on imagery at a distance in excess of 1500 km (900 miles). (2) Another feature of the beacon is its large size. The typical beacon extends over about 5 resolution areas with a characteristic dimension of about 200 m.

Beacon Beams for Deep Turbulence High Energy Laser Beam Directors

DTIC Science & Technology

2012-11-02

variance and nC is the atmospheric refractive index structure constant. The effect of turbulence on the focused beacon beam on target, TR...complete phase conjugation of the beacon beam is accomplished by employing Brillouin enhanced optical four wave mixing. A beacon beam formed by...Naval Research Laboratory Washington, DC 20375-5320 NRL/MR/6790--12-9445 Beacon Beams for Deep Turbulence High Energy Laser Beam Directors P

Determining Locations by Use of Networks of Passive Beacons

NASA Technical Reports Server (NTRS)

Okino, Clayton; Gray, Andrew; Jennings, Esther

2009-01-01

Networks of passive radio beacons spanning moderate-sized terrain areas have been proposed to aid navigation of small robotic aircraft that would be used to explore Saturn s moon Titan. Such networks could also be used on Earth to aid navigation of robotic aircraft, land vehicles, or vessels engaged in exploration or reconnaissance in situations or locations (e.g., underwater locations) in which Global Positioning System (GPS) signals are unreliable or unavailable. Prior to use, it would be necessary to pre-position the beacons at known locations that would be determined by use of one or more precise independent global navigation system(s). Thereafter, while navigating over the area spanned by a given network of passive beacons, an exploratory robot would use the beacons to determine its position precisely relative to the known beacon positions (see figure). If it were necessary for the robot to explore multiple, separated terrain areas spanned by different networks of beacons, the robot could use a long-haul, relatively coarse global navigation system for the lower-precision position determination needed during transit between such areas. The proposed method of precise determination of position of an exploratory robot relative to the positions of passive radio beacons is based partly on the principles of radar and partly on the principles of radio-frequency identification (RFID) tags. The robot would transmit radar-like signals that would be modified and reflected by the passive beacons. The distance to each beacon would be determined from the roundtrip propagation time and/or round-trip phase shift of the signal returning from that beacon. Signals returned from different beacons could be distinguished by means of their RFID characteristics. Alternatively or in addition, the antenna of each beacon could be designed to radiate in a unique pattern that could be identified by the navigation system. Also, alternatively or in addition, sets of identical beacons could be deployed in unique configurations such that the navigation system could identify their unique combinations of radio-frequency reflections as an alternative to leveraging the uniqueness of the RFID tags. The degree of dimensional accuracy would depend not only on the locations of the beacons but also on the number of beacon signals received, the number of samples of each signal, the motion of the robot, and the time intervals between samples. At one extreme, a single sample of the return signal from a single beacon could be used to determine the distance from that beacon and hence to determine that the robot is located somewhere on a sphere, the radius of which equals that distance and the center of which lies at the beacon. In a less extreme example, the three-dimensional position of the robot could be determined with fair precision from a single sample of the signal from each of three beacons. In intermediate cases, position estimates could be refined and/or position ambiguities could be resolved by use of supplementary readings of an altimeter and other instruments aboard the robot.

14 CFR 171.269 - Marker beacon performance requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Marker beacon performance requirements. 171.269 Section 171.269 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Landing System (ISMLS) § 171.269 Marker beacon performance requirements. ISMLS marker beacon equipment...

NOA: A Scalable Multi-Parent Clustering Hierarchy for WSNs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cree, Johnathan V.; Delgado-Frias, Jose; Hughes, Michael A.

2012-08-10

NOA is a multi-parent, N-tiered, hierarchical clustering algorithm that provides a scalable, robust and reliable solution to autonomous configuration of large-scale wireless sensor networks. The novel clustering hierarchy's inherent benefits can be utilized by in-network data processing techniques to provide equally robust, reliable and scalable in-network data processing solutions capable of reducing the amount of data sent to sinks. Utilizing a multi-parent framework, NOA reduces the cost of network setup when compared to hierarchical beaconing solutions by removing the expense of r-hop broadcasting (r is the radius of the cluster) needed to build the network and instead passes network topologymore » information among shared children. NOA2, a two-parent clustering hierarchy solution, and NOA3, the three-parent variant, saw up to an 83% and 72% reduction in overhead, respectively, when compared to performing one round of a one-parent hierarchical beaconing, as well as 92% and 88% less overhead when compared to one round of two- and three-parent hierarchical beaconing hierarchy.« less

Localization with a mobile beacon in underwater acoustic sensor networks.

PubMed

Lee, Sangho; Kim, Kiseon

2012-01-01

Localization is one of the most important issues associated with underwater acoustic sensor networks, especially when sensor nodes are randomly deployed. Given that it is difficult to deploy beacon nodes at predetermined locations, localization schemes with a mobile beacon on the sea surface or along the planned path are inherently convenient, accurate, and energy-efficient. In this paper, we propose a new range-free Localization with a Mobile Beacon (LoMoB). The mobile beacon periodically broadcasts a beacon message containing its location. Sensor nodes are individually localized by passively receiving the beacon messages without inter-node communications. For location estimation, a set of potential locations are obtained as candidates for a node's location and then the node's location is determined through the weighted mean of all the potential locations with the weights computed based on residuals.

Localization with a Mobile Beacon in Underwater Acoustic Sensor Networks

PubMed Central

Lee, Sangho; Kim, Kiseon

2012-01-01

Localization is one of the most important issues associated with underwater acoustic sensor networks, especially when sensor nodes are randomly deployed. Given that it is difficult to deploy beacon nodes at predetermined locations, localization schemes with a mobile beacon on the sea surface or along the planned path are inherently convenient, accurate, and energy-efficient. In this paper, we propose a new range-free Localization with a Mobile Beacon (LoMoB). The mobile beacon periodically broadcasts a beacon message containing its location. Sensor nodes are individually localized by passively receiving the beacon messages without inter-node communications. For location estimation, a set of potential locations are obtained as candidates for a node's location and then the node's location is determined through the weighted mean of all the potential locations with the weights computed based on residuals. PMID:22778597

An Interactive Real-Time Locating System Based on Bluetooth Low-Energy Beacon Network †

PubMed Central

Lin, You-Wei

2018-01-01

The ubiquity of Bluetooth-enabled smartphones and peripherals has brought tremendous convenience to our daily life. In recent years, Bluetooth beacons have also been gaining popularity in implementing a variety of innovative location-based services such as self-guided systems in exhibition centers. However, the broadcast-based beacon technology can only provide unidirectional communication. In case smartphone users would like to respond to the beacon messages, they have to rely on their own mobile Internet connections to send the information back to the backend system. Nevertheless, mobile Internet services may not be always available or too costly. In this work, we develop a real-time locating system based only on the Bluetooth low energy (BLE) technology to support interactive communications by combining the broadcast and mesh topology options to extend the applicability of beacon solutions. Specifically, we turn the smartphone into a beacon device and augment the beacon devices with the capability of forming a mesh network. The implementation result shows that our beacon devices can detect the presence of specific users at specific locations, and then the presence state can be sent to the application server via the relay of beacon devices. Moreover, the application server can send personalized location-based messages to the users, again via the relay of beacon devices. With the capability of relaying messages between the beacon devices, it would be convenient for developers to implement a variety of interactive applications such as tracking VIP customers at the airport, or tracking an elder with Alzheimer’s disease in the neighborhood. PMID:29883386

An Interactive Real-Time Locating System Based on Bluetooth Low-Energy Beacon Network †.

PubMed

Lin, You-Wei; Lin, Chi-Yi

2018-05-21

The ubiquity of Bluetooth-enabled smartphones and peripherals has brought tremendous convenience to our daily life. In recent years, Bluetooth beacons have also been gaining popularity in implementing a variety of innovative location-based services such as self-guided systems in exhibition centers. However, the broadcast-based beacon technology can only provide unidirectional communication. In case smartphone users would like to respond to the beacon messages, they have to rely on their own mobile Internet connections to send the information back to the backend system. Nevertheless, mobile Internet services may not be always available or too costly. In this work, we develop a real-time locating system based only on the Bluetooth low energy (BLE) technology to support interactive communications by combining the broadcast and mesh topology options to extend the applicability of beacon solutions. Specifically, we turn the smartphone into a beacon device and augment the beacon devices with the capability of forming a mesh network. The implementation result shows that our beacon devices can detect the presence of specific users at specific locations, and then the presence state can be sent to the application server via the relay of beacon devices. Moreover, the application server can send personalized location-based messages to the users, again via the relay of beacon devices. With the capability of relaying messages between the beacon devices, it would be convenient for developers to implement a variety of interactive applications such as tracking VIP customers at the airport, or tracking an elder with Alzheimer’s disease in the neighborhood.

14 CFR 171.321 - DME and marker beacon performance requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false DME and marker beacon performance... (MLS) § 171.321 DME and marker beacon performance requirements. (a) The DME equipment must meet the..._regulations/ibr_locations.html. (b) MLS marker beacon equipment must meet the performance requirements...

A Beacon Transmission Power Control Algorithm Based on Wireless Channel Load Forecasting in VANETs.

PubMed

Mo, Yuanfu; Yu, Dexin; Song, Jun; Zheng, Kun; Guo, Yajuan

2015-01-01

In a vehicular ad hoc network (VANET), the periodic exchange of single-hop status information broadcasts (beacon frames) produces channel loading, which causes channel congestion and induces information conflict problems. To guarantee fairness in beacon transmissions from each node and maximum network connectivity, adjustment of the beacon transmission power is an effective method for reducing and preventing channel congestion. In this study, the primary factors that influence wireless channel loading are selected to construct the KF-BCLF, which is a channel load forecasting algorithm based on a recursive Kalman filter and employs multiple regression equation. By pre-adjusting the transmission power based on the forecasted channel load, the channel load was kept within a predefined range; therefore, channel congestion was prevented. Based on this method, the CLF-BTPC, which is a transmission power control algorithm, is proposed. To verify KF-BCLF algorithm, a traffic survey method that involved the collection of floating car data along a major traffic road in Changchun City is employed. By comparing this forecast with the measured channel loads, the proposed KF-BCLF algorithm was proven to be effective. In addition, the CLF-BTPC algorithm is verified by simulating a section of eight-lane highway and a signal-controlled urban intersection. The results of the two verification process indicate that this distributed CLF-BTPC algorithm can effectively control channel load, prevent channel congestion, and enhance the stability and robustness of wireless beacon transmission in a vehicular network.

A Beacon Transmission Power Control Algorithm Based on Wireless Channel Load Forecasting in VANETs

PubMed Central

Mo, Yuanfu; Yu, Dexin; Song, Jun; Zheng, Kun; Guo, Yajuan

2015-01-01

In a vehicular ad hoc network (VANET), the periodic exchange of single-hop status information broadcasts (beacon frames) produces channel loading, which causes channel congestion and induces information conflict problems. To guarantee fairness in beacon transmissions from each node and maximum network connectivity, adjustment of the beacon transmission power is an effective method for reducing and preventing channel congestion. In this study, the primary factors that influence wireless channel loading are selected to construct the KF-BCLF, which is a channel load forecasting algorithm based on a recursive Kalman filter and employs multiple regression equation. By pre-adjusting the transmission power based on the forecasted channel load, the channel load was kept within a predefined range; therefore, channel congestion was prevented. Based on this method, the CLF-BTPC, which is a transmission power control algorithm, is proposed. To verify KF-BCLF algorithm, a traffic survey method that involved the collection of floating car data along a major traffic road in Changchun City is employed. By comparing this forecast with the measured channel loads, the proposed KF-BCLF algorithm was proven to be effective. In addition, the CLF-BTPC algorithm is verified by simulating a section of eight-lane highway and a signal-controlled urban intersection. The results of the two verification process indicate that this distributed CLF-BTPC algorithm can effectively control channel load, prevent channel congestion, and enhance the stability and robustness of wireless beacon transmission in a vehicular network. PMID:26571042

Molecular beacon sequence design algorithm.

PubMed

Monroe, W Todd; Haselton, Frederick R

2003-01-01

A method based on Web-based tools is presented to design optimally functioning molecular beacons. Molecular beacons, fluorogenic hybridization probes, are a powerful tool for the rapid and specific detection of a particular nucleic acid sequence. However, their synthesis costs can be considerable. Since molecular beacon performance is based on its sequence, it is imperative to rationally design an optimal sequence before synthesis. The algorithm presented here uses simple Microsoft Excel formulas and macros to rank candidate sequences. This analysis is carried out using mfold structural predictions along with other free Web-based tools. For smaller laboratories where molecular beacons are not the focus of research, the public domain algorithm described here may be usefully employed to aid in molecular beacon design.

Antarctic field tests of SARSAT personal locater beacons

NASA Technical Reports Server (NTRS)

Bindschadler, Robert

1987-01-01

Field tests of SARSAT personal locater beacons were conducted in the Antarctic to assess the viability of using these beacons to increase the safety of Antarctic field parties. Data were collected on the extent to which dry or wet snow, melting conditions, crevasse walls and snow bridges affected the ability of the SARSAT satellite to calculate an accurate position of the beacon. Average response time between beacon turn on and alert reception in McMurdo was between 4 and 5 hours for these tests. It is concluded that the SARSAT system is viable for Antarctic operations and it is recommended that it be implemented for future field operations. Because of obstruction of line-of-sight between beacon and satellite degrades the accuracy of the location calculation (particularly in wet snow), it is further recommended that field parties have sufficient numbers of beacons to insure that in an emergency, one will be able to operate from the surface.

High-visibility infrared beacons for IFF and combat ID

NASA Astrophysics Data System (ADS)

Pralle, Martin; Puscasu, Irina; Johnson, Edward; Loges, Peter; Melnyk, James

2005-05-01

A new kind of Identification Friend or Foe (IFF) infrared beacon has been demonstrated. The omni-directional beacon consists of a pyramidal array of 1W pulsIR thermal light sources. Operating at a total power of 84W, the beacon can be used to track and identify surface vehicles and personnel with a recognition range of up to 6 miles on the battlefield and in urban environments or the marine boundary layer. Advanced photonic technology enables the beacon to be seen only while using a 3-5 μm or 8-12 μm thermal imaging system. There is no visible or near-IR emission to betray the location of the beacon. The beacon is rugged and will operate from -40 to 50°C ambient temperature, 0-100% relative humidity, 0 - 10,000 ft altitude, and meets MIL-STD 810F and MIL-STD 461E.

Use of a Text Message Program to Raise Type 2 Diabetes Risk Awareness and Promote Health Behavior Change (Part I): Assessment of Participant Reach and Adoption

PubMed Central

Hirzel, Lindsey; Turske, Scott A; Des Jardins, Terrisca R; Yarandi, Hossein; Bondurant, Patricia

2013-01-01

Background There are an estimated 25.8 million American children and adults, equivalent to 8.3% of the US population, living with diabetes. Diabetes is particularly burdensome on minority populations. The use of mobile technologies for reaching broad populations is a promising approach, given its wide footprint and ability to deliver inexpensive personalized messages, to increase awareness of type 2 diabetes and promote behavior changes targeting risk factors associated with type 2 diabetes. As a part of the Beacon Community Cooperative Agreement Program, txt4health, a public-facing mobile health information service, was launched in 3 Beacon Communities: the Southeast Michigan Beacon Community in Detroit, MI, the Greater Cincinnati Beacon Community in Cincinnati, OH, and the Crescent City Beacon Community in New Orleans, LA. Txt4health is a mobile health information service designed to help people understand their risk for type 2 diabetes and become more informed about the steps they can take to lead healthy lives. Objective The purpose of this investigation was to use the RE-AIM framework to document txt4health reach and adoption by focusing on enrollment and participant engagement in program pilots in Southeast Michigan and Greater Cincinnati. Methods We conducted a retrospective records analysis of individual-level txt4health system data from participants in Southeast Michigan and Greater Cincinnati to determine participant usage of txt4health and engagement with the program. Results Results from the retrospective records analysis revealed that 5570 participants initiated the 2-step enrollment process via 1 of 3 enrollment strategies: text message, website, or directly with Beacon staff who signed participants up via the website. In total, 33.00% (1838/5570) of participants completed the 2-step enrollment process and were fully enrolled in the program. All participants (100.00%, 1620/1620) who enrolled via text message completed the entire 2-step enrollment process versus 5.52% (218/3950) of participants who enrolled via website or a Beacon staff member. Of those who fully enrolled, 71.00% (1305/1838) completed the diabetes risk assessment and 74.27% (1365/1838) set an initial weight loss goal. Overall, 39.06% (718/1838) of participants completed all 14 weeks of the program and 56.26% (1034/1838) dropped out before completing all 14 weeks, with the bulk of dropouts occurring in the first 4 weeks. Length of participation varied greatly, ranging from 0-48.7 weeks (median 8.6, mean 15.8, SD 15.8). Wide variability of participant engagement in regards to weekly weight and physical activity was documented. Conclusions Although broadly focused public health text message interventions may have the potential to reach large populations and show high levels of engagement among some users, the level of individual engagement among participants varies widely, suggesting that this type of approach may not be appropriate for all. PMID:24356329

33 CFR 62.23 - Beacons and buoys.

Code of Federal Regulations, 2010 CFR

2010-07-01

... navigation. The primary components of the U.S. Aids to Navigation System are beacons and buoys. (b) Beacons are aids to navigation structures which are permanently fixed to the earth's surface. They range from... UNITED STATES AIDS TO NAVIGATION SYSTEM The U.S. Aids to Navigation System § 62.23 Beacons and buoys. (a...

33 CFR 62.23 - Beacons and buoys.

Code of Federal Regulations, 2011 CFR

2011-07-01

... navigation. The primary components of the U.S. Aids to Navigation System are beacons and buoys. (b) Beacons are aids to navigation structures which are permanently fixed to the earth's surface. They range from... UNITED STATES AIDS TO NAVIGATION SYSTEM The U.S. Aids to Navigation System § 62.23 Beacons and buoys. (a...

33 CFR 62.23 - Beacons and buoys.

Code of Federal Regulations, 2013 CFR

2013-07-01

... navigation. The primary components of the U.S. Aids to Navigation System are beacons and buoys. (b) Beacons are aids to navigation structures which are permanently fixed to the earth's surface. They range from... UNITED STATES AIDS TO NAVIGATION SYSTEM The U.S. Aids to Navigation System § 62.23 Beacons and buoys. (a...

33 CFR 62.23 - Beacons and buoys.

Code of Federal Regulations, 2014 CFR

2014-07-01

... navigation. The primary components of the U.S. Aids to Navigation System are beacons and buoys. (b) Beacons are aids to navigation structures which are permanently fixed to the earth's surface. They range from... UNITED STATES AIDS TO NAVIGATION SYSTEM The U.S. Aids to Navigation System § 62.23 Beacons and buoys. (a...

33 CFR 62.23 - Beacons and buoys.

Code of Federal Regulations, 2012 CFR

2012-07-01

... navigation. The primary components of the U.S. Aids to Navigation System are beacons and buoys. (b) Beacons are aids to navigation structures which are permanently fixed to the earth's surface. They range from... UNITED STATES AIDS TO NAVIGATION SYSTEM The U.S. Aids to Navigation System § 62.23 Beacons and buoys. (a...

Development of a high-precision selenodetic coordinate system for the physical surface of the Moon based on LED beacons on its surface

NASA Astrophysics Data System (ADS)

Shirenin, A. M.; Mazurova, E. M.; Bagrov, A. V.

2016-11-01

The paper presents a mathematical algorithm for processing an array of angular measurements of light beacons on images of the lunar surface onboard a polar artificial lunar satellite (PALS) during the Luna-Glob mission and coordinate-time referencing of the PALS for the development of reference selenocentric coordinate systems. The algorithm makes it possible to obtain angular positions of point light beacons located on the surface of the Moon in selenocentric celestial coordinates. The operation of measurement systems that determine the position and orientation of the PALS during its active existence have been numerically simulated. Recommendations have been made for the optimal use of different types of measurements, including ground radio trajectory measurements, navigational star sensors based on the onboard star catalog, gyroscopic orientation systems, and space videos of the lunar surface.

Visually Detecting the Variation of miR-301a Expression Using Gold Nanoparticle Beacon.

PubMed

Zhang, Ying; Li, Kai; Li, Dandan; Li, Changfeng; Zhang, Bin

2018-02-01

It is well known that microRNA-301a plays an important role in many diseases, as well as is overexpressed in human colon cancer and affects the process of tumorigenesis. Determination of the miR-301a expression provides insight into the mechanism of tumor progression. In this study, we designed a special hairpin deoxyribonucleic acid (DNA) for miR-301a to functionalize gold nanoparticles, which served as a beacon for detecting miR-301a expression. A-quenching efficiency up to 90% was achieved. The beacon we designed in this study can monitor the precise variation of miR-301a expression in vivo. The strategy reported in this study is a promising approach for the measurement of miRNA in living cells. Moreover, it has a great potential in the study of drug screening and discovery.

Power-Efficient Beacon Recognition Method Based on Periodic Wake-Up for Industrial Wireless Devices.

PubMed

Song, Soonyong; Lee, Donghun; Jang, Ingook; Choi, Jinchul; Son, Youngsung

2018-04-17

Energy harvester-integrated wireless devices are attractive for generating semi-permanent power from wasted energy in industrial environments. The energy-harvesting wireless devices may have difficulty in their communication with access points due to insufficient power supply for beacon recognition during network initialization. In this manuscript, we propose a novel method of beacon recognition based on wake-up control to reduce instantaneous power consumption in the initialization procedure. The proposed method applies a moving window for the periodic wake-up of the wireless devices. For unsynchronized wireless devices, beacons are always located in the same positions within each beacon interval even though the starting offsets are unknown. Using these characteristics, the moving window checks the existence of the beacon associated withspecified resources in a beacon interval, checks again for neighboring resources at the next beacon interval, and so on. This method can reduce instantaneous power and generates a surplus of charging time. Thus, the proposed method alleviates the problems of power insufficiency in the network initialization. The feasibility of the proposed method is evaluated using computer simulations of power shortage in various energy-harvesting conditions.

Addressing Beacon re-identification attacks: quantification and mitigation of privacy risks.

PubMed

Raisaro, Jean Louis; Tramèr, Florian; Ji, Zhanglong; Bu, Diyue; Zhao, Yongan; Carey, Knox; Lloyd, David; Sofia, Heidi; Baker, Dixie; Flicek, Paul; Shringarpure, Suyash; Bustamante, Carlos; Wang, Shuang; Jiang, Xiaoqian; Ohno-Machado, Lucila; Tang, Haixu; Wang, XiaoFeng; Hubaux, Jean-Pierre

2017-07-01

The Global Alliance for Genomics and Health (GA4GH) created the Beacon Project as a means of testing the willingness of data holders to share genetic data in the simplest technical context-a query for the presence of a specified nucleotide at a given position within a chromosome. Each participating site (or "beacon") is responsible for assuring that genomic data are exposed through the Beacon service only with the permission of the individual to whom the data pertains and in accordance with the GA4GH policy and standards.While recognizing the inference risks associated with large-scale data aggregation, and the fact that some beacons contain sensitive phenotypic associations that increase privacy risk, the GA4GH adjudged the risk of re-identification based on the binary yes/no allele-presence query responses as acceptable. However, recent work demonstrated that, given a beacon with specific characteristics (including relatively small sample size and an adversary who possesses an individual's whole genome sequence), the individual's membership in a beacon can be inferred through repeated queries for variants present in the individual's genome.In this paper, we propose three practical strategies for reducing re-identification risks in beacons. The first two strategies manipulate the beacon such that the presence of rare alleles is obscured; the third strategy budgets the number of accesses per user for each individual genome. Using a beacon containing data from the 1000 Genomes Project, we demonstrate that the proposed strategies can effectively reduce re-identification risk in beacon-like datasets. © The Author 2017. Published by Oxford University Press on behalf of the American Medical Informatics Association.

Indoor Navigation using Direction Sensor and Beacons

NASA Technical Reports Server (NTRS)

Shields, Joel; Jeganathan, Muthu

2004-01-01

A system for indoor navigation of a mobile robot includes (1) modulated infrared beacons at known positions on the walls and ceiling of a room and (2) a cameralike sensor, comprising a wide-angle lens with a position-sensitive photodetector at the focal plane, mounted in a known position and orientation on the robot. The system also includes a computer running special-purpose software that processes the sensor readings to obtain the position and orientation of the robot in all six degrees of freedom in a coordinate system embedded in the room.

A Place to Grow: Evaluation of the New York City Beacons. Final Report

ERIC Educational Resources Information Center

Warren, Constancia; Feist, Michelle; Nevarez, Nancy

2002-01-01

This study examined six Beacon centers, which are community centers located in public school buildings that offer a range of activities and services to participants of all ages, before and after school, in the evenings, and on weekends. Individual Beacons are managed by community-based organizations. The study examined how the Beacons provided…

A Place To Grow: Evaluation of the New York City Beacons. Summary Report.

ERIC Educational Resources Information Center

Warren, Constancia; Feist, Michelle; Nevarez, Nancy

This study examined six Beacon centers, which are community centers located in public school buildings that offer a range of activities and services to participants of all ages, before and after school, in the evenings, and on weekends. Individual Beacons are managed by community-based organizations. The study examined how the Beacons provided…

33 CFR 149.580 - What are the requirements for a radar beacon?

Code of Federal Regulations, 2011 CFR

2011-07-01

... radar beacon? 149.580 Section 149.580 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Navigation Miscellaneous § 149.580 What are the requirements for a radar beacon? (a) A radar beacon (RACON... Morse code character, the length of which does not exceed 25 percent of the radar range expected to be...

33 CFR 149.580 - What are the requirements for a radar beacon?

Code of Federal Regulations, 2010 CFR

2010-07-01

... radar beacon? 149.580 Section 149.580 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Navigation Miscellaneous § 149.580 What are the requirements for a radar beacon? (a) A radar beacon (RACON... Morse code character, the length of which does not exceed 25 percent of the radar range expected to be...

Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids.

PubMed

Engelen, Wouter; van de Wiel, Kayleigh M; Meijer, Lenny H H; Saha, Bedabrata; Merkx, Maarten

2017-03-02

Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.

33 CFR 149.580 - What are the requirements for a radar beacon?

Code of Federal Regulations, 2013 CFR

2013-07-01

... radar beacon? 149.580 Section 149.580 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Navigation Miscellaneous § 149.580 What are the requirements for a radar beacon? (a) A radar beacon (RACON... Morse code character, the length of which does not exceed 25 percent of the radar range expected to be...

33 CFR 149.580 - What are the requirements for a radar beacon?

Code of Federal Regulations, 2014 CFR

2014-07-01

... radar beacon? 149.580 Section 149.580 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Navigation Miscellaneous § 149.580 What are the requirements for a radar beacon? (a) A radar beacon (RACON... Morse code character, the length of which does not exceed 25 percent of the radar range expected to be...

33 CFR 149.580 - What are the requirements for a radar beacon?

Code of Federal Regulations, 2012 CFR

2012-07-01

... radar beacon? 149.580 Section 149.580 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Navigation Miscellaneous § 149.580 What are the requirements for a radar beacon? (a) A radar beacon (RACON... Morse code character, the length of which does not exceed 25 percent of the radar range expected to be...

Polarization tracking system for free-space optical communication, including quantum communication

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nordholt, Jane Elizabeth; Newell, Raymond Thorson; Peterson, Charles Glen

Quantum communication transmitters include beacon lasers that transmit a beacon optical signal in a predetermined state of polarization such as one of the states of polarization of a quantum communication basis. Changes in the beacon polarization are detected at a receiver, and a retarder is adjusted so that the states of polarization in a received quantum communication optical signal are matched to basis polarizations. The beacon and QC signals can be at different wavelengths so that the beacon does not interfere with detection and decoding of the QC optical signal.

Addressing Beacon re-identification attacks: quantification and mitigation of privacy risks

PubMed Central

Zhao, Yongan; Carey, Knox; Lloyd, David; Sofia, Heidi; Baker, Dixie; Flicek, Paul; Shringarpure, Suyash; Bustamante, Carlos; Wang, Shuang; Jiang, Xiaoqian; Ohno-Machado, Lucila; Tang, Haixu; Wang, XiaoFeng; Hubaux, Jean-Pierre

2018-01-01

The Global Alliance for Genomics and Health (GA4GH) created the Beacon Project as a means of testing the willingness of data holders to share genetic data in the simplest technical context—a query for the presence of a specified nucleotide at a given position within a chromosome. Each participating site (or “beacon”) is responsible for assuring that genomic data are exposed through the Beacon service only with the permission of the individual to whom the data pertains and in accordance with the GA4GH policy and standards. While recognizing the inference risks associated with large-scale data aggregation, and the fact that some beacons contain sensitive phenotypic associations that increase privacy risk, the GA4GH adjudged the risk of re-identification based on the binary yes/no allele-presence query responses as acceptable. However, recent work demonstrated that, given a beacon with specific characteristics (including relatively small sample size and an adversary who possesses an individual’s whole genome sequence), the individual’s membership in a beacon can be inferred through repeated queries for variants present in the individual’s genome. In this paper, we propose three practical strategies for reducing re-identification risks in beacons. The first two strategies manipulate the beacon such that the presence of rare alleles is obscured; the third strategy budgets the number of accesses per user for each individual genome. Using a beacon containing data from the 1000 Genomes Project, we demonstrate that the proposed strategies can effectively reduce re-identification risk in beacon-like datasets. PMID:28339683

A search for optical beacons: implications of null results.

PubMed

Blair, David G; Zadnik, Marjan G

2002-01-01

Over the past few years a series of searches for interstellar radio beacons have taken place using the Parkes radio telescope. Here we report hitherto unpublished results from a search for optical beacons from 60 solar-type stars using the Perth-Lowell telescope. We discuss the significance of the null results from these searches, all of which were based on the interstellar contact channel hypothesis. While the null results of all searches to date can be explained simply by the nonexistence of electromagnetically communicating life elsewhere in the Milky Way, four other possible explanations that do not preclude its existence are proposed: (1) Extraterrestrial civilizations desiring to make contact through the use of electromagnetic beacons have a very low density in the Milky Way. (2) The interstellar contact channel hypothesis is incorrect, and beacons exist at frequencies that have not yet been searched. (3) The search has been incomplete in terms of sensitivity and/or target directions: Beacons exist, but more sensitive equipment and/or more searching is needed to achieve success. (4) The search has occurred before beacon signals can be expected to have arrived at the Earth, and beacon signals may be expected in the future. Based on consideration of the technology required for extraterrestrial civilizations to identify target planets, we argue that the fourth possibility is likely to be valid and that powerful, easily detectable beacons could be received in coming centuries.

Underwater Acoustic Beacon Location System

DTIC Science & Technology

2016-12-23

300087 1 of 31 UNDERWATER ACOUSTIC BEACON LOCATION SYSTEM [0001] The present application claims the benefit of United States Provisional...Application Serial Number 62/297,179 filed on February 19, 2016 by the inventor, Steven E. Crocker and entitled “ Underwater Acoustic Beacon...search for and can locate an underwater location beacon. Attorney Docket No. 300087 2 of 31 (2) Description of the Prior Art [0005

Power-Efficient Beacon Recognition Method Based on Periodic Wake-Up for Industrial Wireless Devices

PubMed Central

Lee, Donghun; Jang, Ingook; Choi, Jinchul; Son, Youngsung

2018-01-01

Energy harvester-integrated wireless devices are attractive for generating semi-permanent power from wasted energy in industrial environments. The energy-harvesting wireless devices may have difficulty in their communication with access points due to insufficient power supply for beacon recognition during network initialization. In this manuscript, we propose a novel method of beacon recognition based on wake-up control to reduce instantaneous power consumption in the initialization procedure. The proposed method applies a moving window for the periodic wake-up of the wireless devices. For unsynchronized wireless devices, beacons are always located in the same positions within each beacon interval even though the starting offsets are unknown. Using these characteristics, the moving window checks the existence of the beacon associated withspecified resources in a beacon interval, checks again for neighboring resources at the next beacon interval, and so on. This method can reduce instantaneous power and generates a surplus of charging time. Thus, the proposed method alleviates the problems of power insufficiency in the network initialization. The feasibility of the proposed method is evaluated using computer simulations of power shortage in various energy-harvesting conditions. PMID:29673206

The CERTO Beacon on CASSIOPE/e-POP and Experiments Using High-Power HF Ionospheric Heaters

NASA Astrophysics Data System (ADS)

Siefring, Carl L.; Bernhardt, Paul A.; James, H. Gordon; Parris, Richard Todd

2015-06-01

A new Coherent Electromagnetic Radio Tomography (CERTO) beacon is on the CASSIOPE satellite and part of the enhanced-Polar Outflow Probe (e-POP) suite of scientific instruments. CERTO signals can be used to measure ionospheric Total Electron Content (TEC) and radio scintillations along propagation paths between CERTO and receivers. The combination of CERTO and the array of e-POP in-situ diagnostics form a powerful tool for studying ionospheric plasma processes that have not been previously possible. Of note, the combination CERTO and the Radio Receiver Instrument (RRI), a modern digital receiver, which measures between 10 Hz to 18 MHz in selectable bands allows for innovative High Frequency (HF) radio propagation experiments. The use of high-power HF ionospheric heating facilities for such experiments further allows for repeatable studies of a number of important plasma processes. The new CERTO beacon transmits un-modulated, phase-coherent waves at 150, 400, and 1067 MHz with either right-hand-circular or linear polarization and TEC is measured using either differential phase and/or Faraday rotation. With a linear array of CERTO receivers, TEC data can be used for tomographic imaging of the ionosphere yielding two-dimensional maps of the plasma below the satellite orbit. In addition, the three CERTO frequencies cover a wide range for determination of radio scintillation effects caused by diffraction from propagation through ionospheric irregularities. We will describe the CERTO beacon and several potential innovative experiments using HF heating facilities in conjunction with CERTO, the RRI and other e-POP instruments.

From Beacon Valley, Antarctica to Mars: Bringing the PolarTREC Teacher Research Experience to the Classroom

NASA Astrophysics Data System (ADS)

Hams, J. E.; Marchant, D. R.

2009-12-01

PolarTREC is an educational research experience in which K-14 teachers participate in polar research with scientists as a pathway to improving science education. The program is funded by the National Science Foundation and managed by the Arctic Research Consortium of the United States. Teachers share their experiences with scientists, educators, communities, and students around the world during the expeditions through online journals, and translate the experience to the classroom by developing curricula based on the research expedition. A 2008 PolarTREC teacher research experience involved drilling through buried ice beneath glaciers in one of the Dry Valleys located in the cold-polar desert region of Antarctica. PolarTREC teacher Jacquelyn Hams accompanied Boston University researchers Dr. David Marchant and Sean Mackay to Beacon Valley, Antarctica. Beacon Valley is significant because the ice beneath it is estimated to be over several million years in age, making it the oldest ice known on this planet and it is one of the most Mars-like climatic environments and landscapes on Earth. In order to translate this unique environment to the classroom, lesson plans were created for students to study the topography, glacial landforms, and wind patterns of Beacon Valley. Students also examine the same imagery that scientists use to study the processes that operate in the extreme environments of Antarctica and Mars. The topographic maps and aerial images of Beacon Valley were obtained from the NASA LIMA (Landsat Image Mosaic of Antarctica) website and Mars imagery was obtained from the University of Arizona HiRISE (Hi Resolution Imaging Science Experiment) website. Wind data was downloaded from an anemometer in Beacon Valley and provided courtesy of Sean Mackay of Boston University. The lesson plans are available through the PolarTREC website or by contacting Jacquelyn Hams at hamsje@lavc.edu.

MO-FG-BRA-06: Electromagnetic Beacon Insertion in Lung Cancer Patients and Resultant Surrogacy Errors for Dynamic MLC Tumour Tracking

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hardcastle, N; Booth, J; Caillet, V

Purpose: To assess endo-bronchial electromagnetic beacon insertion and to quantify the geometric accuracy of using beacons as a surrogate for tumour motion in real-time multileaf collimator (MLC) tracking of lung tumours. Methods: The LIGHT SABR trial is a world-first clinical trial in which the MLC leaves move with lung tumours in real time on a standard linear accelerator. Tracking is performed based on implanted electromagnetic beacons (CalypsoTM, Varian Medical Systems, USA) as a surrogate for tumour motion. Five patients have been treated and have each had three beacons implanted endo-bronchially under fluoroscopic guidance. The centre of mass (C.O.M) has beenmore » used to adapt the MLC in real-time. The geometric error in using the beacon C.O.M as a surrogate for tumour motion was measured by measuring the tumour and beacon C.O.M in all phases of the respiratory cycle of a 4DCT. The surrogacy error was defined as the difference in beacon and tumour C.O.M relative to the reference phase (maximum exhale). Results: All five patients have had three beacons successfully implanted with no migration between simulation and end of treatment. Beacon placement relative to tumour C.O.M varied from 14 to 74 mm and in one patient spanned two lobes. Surrogacy error was measured in each patient on the simulation 4DCT and ranged from 0 to 3 mm. Surrogacy error as measured on 4DCT was subject to artefacts in mid-ventilation phases. Surrogacy error was a function of breathing phase and was typically larger at maximum inhale. Conclusion: Beacon placement and thus surrogacy error is a major component of geometric uncertainty in MLC tracking of lung tumours. Surrogacy error must be measured on each patient and incorporated into margin calculation. Reduction of surrogacy error is limited by airway anatomy, however should be taken into consideration when performing beacon insertion and planning. This research is funded by Varian Medical Systems via a collaborative research agreement.« less

Design and Practical Evaluation of a Family of Lightweight Protocols for Heterogeneous Sensing through BLE Beacons in IoT Telemetry Applications.

PubMed

Hernández-Rojas, Dixys L; Fernández-Caramés, Tiago M; Fraga-Lamas, Paula; Escudero, Carlos J

2017-12-27

The Internet of Things (IoT) involves a wide variety of heterogeneous technologies and resource-constrained devices that interact with each other. Due to such constraints, IoT devices usually require lightweight protocols that optimize the use of resources and energy consumption. Among the different commercial IoT devices, Bluetooth and Bluetooth Low Energy (BLE)-based beacons, which broadcast periodically certain data packets to notify their presence, have experienced a remarkable growth, specially due to their application in indoor positioning systems. This article proposes a family of protocols named Lightweight Protocol for Sensors (LP4S) that provides fast responses and enables plug-and-play mechanisms that allow IoT telemetry systems to discover new nodes and to describe and auto-register the sensors and actuators connected to a beacon. Thus, three protocols are defined depending on the beacon hardware characteristics: LP4S-6 (for resource-constraint beacons), LP4S-X (for more powerful beacons) and LP4S-J (for beacons able to run complex firmware). In order to demonstrate the capabilities of the designed protocols, the most restrictive (LP4S-6) is tested after implementing it for a telemetry application in a beacon based on Eddystone (Google's open beacon format). Thus, the beacon specification is extended in order to increase its ability to manage unlimited sensors in a telemetry system without interfering in its normal operation with Eddystone frames. The performed experiments show the feasibility of the proposed solution and its superiority, in terms of latency and energy consumption, with respect to approaches based on Generic Attribute Profile (GATT) when multiple users connect to a mote or in scenarios where latency is not a restriction, but where low-energy consumption is essential.

Design and Practical Evaluation of a Family of Lightweight Protocols for Heterogeneous Sensing through BLE Beacons in IoT Telemetry Applications

PubMed Central

2017-01-01

The Internet of Things (IoT) involves a wide variety of heterogeneous technologies and resource-constrained devices that interact with each other. Due to such constraints, IoT devices usually require lightweight protocols that optimize the use of resources and energy consumption. Among the different commercial IoT devices, Bluetooth and Bluetooth Low Energy (BLE)-based beacons, which broadcast periodically certain data packets to notify their presence, have experienced a remarkable growth, specially due to their application in indoor positioning systems. This article proposes a family of protocols named Lightweight Protocol for Sensors (LP4S) that provides fast responses and enables plug-and-play mechanisms that allow IoT telemetry systems to discover new nodes and to describe and auto-register the sensors and actuators connected to a beacon. Thus, three protocols are defined depending on the beacon hardware characteristics: LP4S-6 (for resource-constraint beacons), LP4S-X (for more powerful beacons) and LP4S-J (for beacons able to run complex firmware). In order to demonstrate the capabilities of the designed protocols, the most restrictive (LP4S-6) is tested after implementing it for a telemetry application in a beacon based on Eddystone (Google’s open beacon format). Thus, the beacon specification is extended in order to increase its ability to manage unlimited sensors in a telemetry system without interfering in its normal operation with Eddystone frames. The performed experiments show the feasibility of the proposed solution and its superiority, in terms of latency and energy consumption, with respect to approaches based on Generic Attribute Profile (GATT) when multiple users connect to a mote or in scenarios where latency is not a restriction, but where low-energy consumption is essential. PMID:29280975

SPHERES ultrasound beacon tester floats in Node 1 during Expedition 8

NASA Image and Video Library

2004-03-24

ISS008-E-19132 (24 March 2004) --- The Synchronized Position Hold, Engage, Reorient, Experimental Satellites (SPHERES) Beacon / Beacon Tester floats in the Unity node of the International Space Station.

Pedestrian and motorists' actions at pedestrian hybrid beacon sites: findings from a pilot study.

PubMed

Pulugurtha, Srinivas S; Self, Debbie R

2015-01-01

This paper focuses on an analysis of pedestrian and motorists' actions at sites with pedestrian hybrid beacons and assesses their effectiveness in improving the safety of pedestrians. Descriptive and statistical analyses (one-tail two-sample T-test and two-proportion Z-test) were conducted using field data collected during morning and evening peak hours at three study sites in the city of Charlotte, NC, before and after the installation of pedestrian hybrid beacons. Further, an analysis was conducted to assess the change in pedestrian and motorists' actions over time (before the installation; 1 month, 3 months, 6 months, and 12 months after the installation). Results showed an increase in average traffic speed at one of the pedestrian hybrid beacon sites while no specific trends were observed at the other two pedestrian hybrid beacon sites. A decrease in the number of motorists not yielding to pedestrians, pedestrians trapped in the middle of the street, and pedestrian-vehicle conflicts were observed at all the three pedestrian hybrid beacon sites. The installation of pedestrian hybrid beacons did not have a negative effect on pedestrian actions at two out of the three sites. Improvements seem to be relatively more consistent 3 months after the installation of the pedestrian hybrid beacon.

Development of a Universal RNA Beacon for Exogenous Gene Detection

PubMed Central

Guo, Yuanjian; Lu, Zhongju; Cohen, Ira Stephen

2015-01-01

Stem cell therapy requires a nontoxic and high-throughput method to achieve a pure cell population to prevent teratomas that can occur if even one cell in the implant has not been transformed. A promising method to detect and separate cells expressing a particular gene is RNA beacon technology. However, developing a successful, specific beacon to a particular transfected gene can take months to develop and in some cases is impossible. Here, we report on an off-the-shelf universal beacon that decreases the time and cost of applying beacon technology to select any living cell population transfected with an exogenous gene. PMID:25769653

Development of a universal RNA beacon for exogenous gene detection.

PubMed

Guo, Yuanjian; Lu, Zhongju; Cohen, Ira Stephen; Scarlata, Suzanne

2015-05-01

Stem cell therapy requires a nontoxic and high-throughput method to achieve a pure cell population to prevent teratomas that can occur if even one cell in the implant has not been transformed. A promising method to detect and separate cells expressing a particular gene is RNA beacon technology. However, developing a successful, specific beacon to a particular transfected gene can take months to develop and in some cases is impossible. Here, we report on an off-the-shelf universal beacon that decreases the time and cost of applying beacon technology to select any living cell population transfected with an exogenous gene. ©AlphaMed Press.

Rayleigh beacon for measuring the surface profile of a radio telescope.

PubMed

Padin, S

2014-12-01

Millimeter-wavelength Rayleigh scattering from water droplets in a cloud is proposed as a means of generating a bright beacon for measuring the surface profile of a radio telescope. A λ=3  mm transmitter, with an output power of a few watts, illuminating a stratiform cloud, can generate a beacon with the same flux as Mars in 10 GHz bandwidth, but the beacon has a narrow line width, so it is extremely bright. The key advantage of the beacon is that it can be used at any time, and positioned anywhere in the sky, as long as there are clouds.

Q-Band (37-41 GHz) Satellite Beacon Architecture for RF Propagation Experiments

NASA Technical Reports Server (NTRS)

Simmons, Rainee N.; Wintucky, Edwin G.

2012-01-01

In this paper, the design of a beacon transmitter that will be flown as a hosted payload on a geostationary satellite to enable propagation experiments at Q-band (37-41 GHz) frequencies is presented. The beacon uses a phased locked loop stabilized dielectric resonator oscillator and a solid-state power amplifier to achieve the desired output power. The satellite beacon antenna is configured as an offset-fed cut-paraboloidal reflector.

Q-Band (37 to 41 GHz) Satellite Beacon Architecture for RF Propagation Experiments

NASA Technical Reports Server (NTRS)

Simons, Rainee N.; Wintucky, Edwin G.

2014-01-01

In this paper, the design of a beacon transmitter that will be flown as a hosted payload on a geostationary satellite to enable propagation experiments at Q-band (37 to 41 GHz) frequencies is presented. The beacon uses a phased locked loop stabilized dielectric resonator oscillator and a solid-state power amplifier to achieve the desired output power. The satellite beacon antenna is configured as an offset-fed cutparaboloidal reflector.

Research on the electro-optical assistant landing system based on the dual camera photogrammetry algorithm

NASA Astrophysics Data System (ADS)

Mi, Yuhe; Huang, Yifan; Li, Lin

2015-08-01

Based on the location technique of beacon photogrammetry, Dual Camera Photogrammetry (DCP) algorithm was used to assist helicopters landing on the ship. In this paper, ZEMAX was used to simulate the two Charge Coupled Device (CCD) cameras imaging four beacons on both sides of the helicopter and output the image to MATLAB. Target coordinate systems, image pixel coordinate systems, world coordinate systems and camera coordinate systems were established respectively. According to the ideal pin-hole imaging model, the rotation matrix and translation vector of the target coordinate systems and the camera coordinate systems could be obtained by using MATLAB to process the image information and calculate the linear equations. On the basis mentioned above, ambient temperature and the positions of the beacons and cameras were changed in ZEMAX to test the accuracy of the DCP algorithm in complex sea status. The numerical simulation shows that in complex sea status, the position measurement accuracy can meet the requirements of the project.

Design and implementation of Bluetooth beacon in mobile payment system

NASA Astrophysics Data System (ADS)

Han, Tiantian; Ding, Lei

2017-08-01

The current line of payment means, mainly in the following ways, cash payment, credit card payment, WeChat Alipay sweep payment. There are many inconvenience in Cash payment, large amounts of cash inconvenience to carry, count the money to spend time and effort, true and false banknotes difficult to distinguish, ticket settlement easy to go wrong. Credit card payment is relatively time-consuming, and WeChat Alipay sweep payment need to sweep. Therefore, the design of a convenient, fast payment to meet the line to pay the demand is particularly important. Based on the characteristics of BLE Bluetooth wireless communication technology, this paper designs a kind of payment method based on Bluetooth beacon. Through the Bluetooth beacon broadcast consumption, consumers only need to open the relevant APP in the Android client, and you can get Bluetooth via mobile phone Bluetooth the amount of consumption of the standard broadcast, in accordance with the corresponding payment platform to complete the payment process, which pay less time to improve the efficiency of payment.

BBIS: Beacon Bus Information System

NASA Astrophysics Data System (ADS)

Kasim, Shahreen; Hafit, Hanayanti; Pei Juin, Kong; Afizah Afif, Zehan; Hashim, Rathiah; Ruslai, Husni; Jahidin, Kamaruzzaman; Syafwan Arshad, Mohammad

2016-11-01

Lack of bus information for example bus timetable, status of the bus and messy advertisement on bulletin board at the bus stop will give negative impact to tourist. Therefore, a real-time update bus information bulletin board provides all information needed so that passengers can save their bus information searching time. Supported with Android or iOS, Beacon Bus Information System (BBIS) provides bus information between Batu Pahat and Kluang area. BBIS is a system that implements physical web technology and interaction on demand. It built on Backend-as-a-Service, a cloud solution and Firebase non relational database as data persistence backend and syncs between user client in the real-time. People walk through bus stop with smart device and do not require any application. Bluetooth Beacon is used to achieve smart device's best performance of data sharing. Intellij IDEA 15 is one of the tools that that used to develop the BBIS system. Multi-language included front end and backend supported Integration development environment (IDE) helped to speed up integration process.

Searching for Cost-Optimized Interstellar Beacons

NASA Astrophysics Data System (ADS)

Benford, Gregory; Benford, James; Benford, Dominic

2010-06-01

What would SETI beacon transmitters be like if built by civilizations that had a variety of motives but cared about cost? In a companion paper, we presented how, for fixed power density in the far field, a cost-optimum interstellar beacon system could be built. Here, we consider how we should search for a beacon if it were produced by a civilization similar to ours. High-power transmitters could be built for a wide variety of motives other than the need for two-way communication; this would include beacons built to be seen over thousands of light-years. Extraterrestrial beacon builders would likely have to contend with economic pressures just as their terrestrial counterparts do. Cost, spectral lines near 1 GHz, and interstellar scintillation favor radiating frequencies substantially above the classic "water hole." Therefore, the transmission strategy for a distant, cost-conscious beacon would be a rapid scan of the galactic plane with the intent to cover the angular space. Such pulses would be infrequent events for the receiver. Such beacons built by distant, advanced, wealthy societies would have very different characteristics from what SETI researchers seek. Future searches should pay special attention to areas along the galactic disk where SETI searches have seen coherent signals that have not recurred on the limited listening time intervals we have used. We will need to wait for recurring events that may arriarrive in intermittent bursts. Several new SETI search strategies have emerged from these ideas. We propose a new test for beacons that is based on the Life Plane hypotheses.

Searching for Cost-Optimized Interstellar Beacons

NASA Technical Reports Server (NTRS)

Benford, Gregory; Benford, James; Benford, Dominic

2010-01-01

What would SETI beacon transmitters be like if built by civilizations that had a variety of motives but cared about cost? In a companion paper, we presented how, for fixed power density in the far field, a cost-optimum interstellar beacon system could be built. Here, we consider how we should search for a beacon if it were produced by a civilization similar to ours. High-power transmitters could be built for a wide variety of motives other than the need for two-way communication, this would include beacons built to be seen over thousands of light-years. Extraterrestrial beacon builders would likely have to contend with economic pressures just as their terrestrial counterparts do. Cost, spectral lines near 1GHz, and interstellar scintillation favor radiating frequencies substantially above the classic "water hole." Therefore, the transmission strategy for a distant, cost-conscious beacon would be a rapid scan of the galactic plane with the intent to cover the angular space. Such pulses would be infrequent events for the receiver. Such beacons built by distant, advanced, wealthy societies would have very different characteristics from what SETI researchers seek. Future searches should pay special attention to areas along the galactic disk where SETI searches have seen coherent signals that have not recurred on the limited listening time intervals we have used. We will need to wait for recurring events that may arrive in intermittent bursts. Several new SETI search strategies have emerged from these ideas. We propose a new test for beacons that is based on the Life Plane hypotheses.

Searching for cost-optimized interstellar beacons.

PubMed

Benford, Gregory; Benford, James; Benford, Dominic

2010-06-01

What would SETI beacon transmitters be like if built by civilizations that had a variety of motives but cared about cost? In a companion paper, we presented how, for fixed power density in the far field, a cost-optimum interstellar beacon system could be built. Here, we consider how we should search for a beacon if it were produced by a civilization similar to ours. High-power transmitters could be built for a wide variety of motives other than the need for two-way communication; this would include beacons built to be seen over thousands of light-years. Extraterrestrial beacon builders would likely have to contend with economic pressures just as their terrestrial counterparts do. Cost, spectral lines near 1 GHz, and interstellar scintillation favor radiating frequencies substantially above the classic "water hole." Therefore, the transmission strategy for a distant, cost-conscious beacon would be a rapid scan of the galactic plane with the intent to cover the angular space. Such pulses would be infrequent events for the receiver. Such beacons built by distant, advanced, wealthy societies would have very different characteristics from what SETI researchers seek. Future searches should pay special attention to areas along the galactic disk where SETI searches have seen coherent signals that have not recurred on the limited listening time intervals we have used. We will need to wait for recurring events that may arrive in intermittent bursts. Several new SETI search strategies have emerged from these ideas. We propose a new test for beacons that is based on the Life Plane hypotheses.

Lessons Learned During Implementation and Early Operations of the DS1 Beacon Monitor Experiment

NASA Technical Reports Server (NTRS)

Sherwood, Rob; Wyatt, Jay; Hotz, Henry; Schlutsmeyer, Alan; Sue, Miles

1998-01-01

A new approach to mission operations will be flight validated on NASA's New Millennium Program Deep Space One (DS1) mission which launched in October 1998. The Beacon Monitor Operations Technology is aimed at decreasing the total volume of downlinked engineering telemetry by reducing the frequency of downlink and the volume of data received per pass. Cost savings are achieved by reducing the amount of routine telemetry processing and analysis performed by ground staff. The technology is required for upcoming NASA missions to Pluto, Europa, and possibly some other missions. With beacon monitoring, the spacecraft will assess its own health and will transmit one of four beacon messages each representing a unique frequency tone to inform the ground how urgent it is to track the spacecraft for telemetry. If all conditions are nominal, the tone provides periodic assurance to ground personnel that the mission is proceeding as planned without having to receive and analyze downlinked telemetry. If there is a problem, the tone will indicate that tracking is required and the resulting telemetry will contain a concise summary of what has occurred since the last telemetry pass. The primary components of the technology are a tone monitoring technology, AI-based software for onboard engineering data summarization, and a ground response system. In addition, there is a ground visualization system for telemetry summaries. This paper includes a description of the Beacon monitor concept, the trade-offs with adapting that concept as a technology experiment, the current state of the resulting implementation on DS1, and our lessons learned during the initial checkout phase of the mission. Applicability to future missions is also included.

The utah beacon experience: integrating quality improvement, health information technology, and practice facilitation to improve diabetes outcomes in small health care facilities.

PubMed

Tennison, Janet; Rajeev, Deepthi; Woolsey, Sarah; Black, Jeff; Oostema, Steven J; North, Christie

2014-01-01

The Utah Improving Care through Connectivity and Collaboration (IC3) Beacon community (2010-2013) was spearheaded by HealthInsight, a nonprofit, community-based organization. One of the main objectives of IC(3) was to improve health care provided to patients with diabetes in three Utah counties, collaborating with 21 independent smaller clinics and two large health care enterprises. This paper will focus on the use of health information technology (HIT) and practice facilitation to develop and implement new care processes to improve clinic workflow and ultimately improve patients' diabetes outcomes at 21 participating smaller, independent clinics. Early in the project, we learned that most of the 21 clinics did not have the resources needed to successfully implement quality improvement (QI) initiatives. IC(3) helped clinics effectively use data generated from their electronic health records (EHRs) to design and implement interventions to improve patients' diabetes outcomes. This close coupling of HIT, expert practice facilitation, and Learning Collaboratives was found to be especially valuable in clinics with limited resources. Through this process we learned that (1) an extensive readiness assessment improved clinic retention, (2) clinic champions were important for a successful collaboration, and (3) current EHR systems have limited functionality to assist in QI initiatives. In general, smaller, independent clinics lack knowledge and experience with QI and have limited HIT experience to improve patient care using electronic clinical data. Additionally, future projects like IC(3) Beacon will be instrumental in changing clinic culture so that QI is integrated into routine workflow. Our efforts led to significant changes in how practice staff optimized their EHRs to manage and improve diabetes care, while establishing the framework for sustainability. Some of the IC(3) Beacon practices are currently smoothly transitioning to new models of care such as Patient-Centered Medical Homes. Thus, IC(3) Beacon has been instrumental in creating a strong community partnership among various organizations to meet the shared vision of better health and lower costs, and the experience over the last few years has helped the community prepare for the changing health care landscape.

The Utah Beacon Experience: Integrating Quality Improvement, Health Information Technology, and Practice Facilitation to Improve Diabetes Outcomes in Small Health Care Facilities

PubMed Central

Tennison, Janet; Rajeev, Deepthi; Woolsey, Sarah; Black, Jeff; Oostema, Steven J.; North, Christie

2014-01-01

Purpose: The Utah Improving Care through Connectivity and Collaboration (IC3) Beacon community (2010–2013) was spearheaded by HealthInsight, a nonprofit, community-based organization. One of the main objectives of IC3 was to improve health care provided to patients with diabetes in three Utah counties, collaborating with 21 independent smaller clinics and two large health care enterprises. This paper will focus on the use of health information technology (HIT) and practice facilitation to develop and implement new care processes to improve clinic workflow and ultimately improve patients’ diabetes outcomes at 21 participating smaller, independent clinics. Innovation: Early in the project, we learned that most of the 21 clinics did not have the resources needed to successfully implement quality improvement (QI) initiatives. IC3 helped clinics effectively use data generated from their electronic health records (EHRs) to design and implement interventions to improve patients’ diabetes outcomes. This close coupling of HIT, expert practice facilitation, and Learning Collaboratives was found to be especially valuable in clinics with limited resources. Findings: Through this process we learned that (1) an extensive readiness assessment improved clinic retention, (2) clinic champions were important for a successful collaboration, and (3) current EHR systems have limited functionality to assist in QI initiatives. In general, smaller, independent clinics lack knowledge and experience with QI and have limited HIT experience to improve patient care using electronic clinical data. Additionally, future projects like IC3 Beacon will be instrumental in changing clinic culture so that QI is integrated into routine workflow. Conclusion and Discussion: Our efforts led to significant changes in how practice staff optimized their EHRs to manage and improve diabetes care, while establishing the framework for sustainability. Some of the IC3 Beacon practices are currently smoothly transitioning to new models of care such as Patient-Centered Medical Homes. Thus, IC3 Beacon has been instrumental in creating a strong community partnership among various organizations to meet the shared vision of better health and lower costs, and the experience over the last few years has helped the community prepare for the changing health care landscape. PMID:25848624

DNA aptamer beacon assay for C-telopeptide and handheld fluorometer to monitor bone resorption.

PubMed

Bruno, John Gordon; Carrillo, Maria P; Phillips, Taylor; Hanson, Douglas; Bohmann, Jonathan A

2011-09-01

A novel DNA aptamer beacon is described for quantification of a 26-amino acid C-telopeptide (CTx) of human type I bone collagen. One aptamer sequence and its reverse complement dominated the aptamer pool (31.6% of sequenced clones). Secondary structures of these aptamers were examined for potential binding pockets. Three-dimensional computer models which analyzed docking topologies and binding energies were in agreement with empirical fluorescence experiments used to select one candidate loop for beacon assay development. All loop structures from the aptamer finalists were end-labeled with TYE 665 and Iowa Black quencher for comparison of beacon fluorescence levels as a function of CTx concentration. The optimal beacon, designated CTx 2R-2h yielded a low ng/ml limit of detection using a commercially available handheld fluorometer. The CTx aptamer beacon bound full-length 26-amino acid CTx peptide, but not a shorter 8-amino acid segment of CTx peptide which is a common target for commercial CTx ELISA kits. The prototype assay was shown to detect CTx peptide from human urine after creatinine and urea were removed by size-exclusion chromatography to prevent nonspecific denaturing of the aptamer beacon. This work demonstrates the potential of aptamer beacons to be utilized for rapid and sensitive bone health monitoring in a handheld or point-of-care format.

Visual detection of STAT5B gene expression in living cell using the hairpin DNA modified gold nanoparticle beacon.

PubMed

Xue, Jianpeng; Shan, Lingling; Chen, Haiyan; Li, Yang; Zhu, Hongyan; Deng, Dawei; Qian, Zhiyu; Achilefu, Samuel; Gu, Yueqing

2013-03-15

Signal transducer and activator of transcription 5B (STAT5B) is an important protein in JAK-STAT signaling pathway that is responsible for the metastasis and proliferation of tumor cells. Determination of the STAT5B messenger Ribonucleic Acid (mRNA) relating to the STAT5B expression provides insight into the mechanism of tumor progression. In this study, we designed and used a special hairpin deoxyribonucleic acid (DNA) for human STAT5B mRNA to functionalize gold nanoparticles, which served as a beacon for detecting human STAT5B expression. Up to 90% quenching efficiency was achieved. Upon hybridizing with the target mRNA, the hairpin DNA modified gold nanoparticle beacons (hDAuNP beacons) release the fluorophores attached at 5' end of the oligonucleotide sequence. The fluorescence properties of the beacon before and after the hybridization with the complementary DNA were confirmed in vitro. The stability of hDAuNP beacons against degradation by DNase I and GSH indicated that the prepared beacon is stable inside cells. The detected fluorescence in MCF-7 cancer cells correlates with the specific STAT5B mRNA expression, which is consistent with the result from PCR measurement. Fluorescence microscopy showed that the hDAuNP beacons internalized in cells without using transfection agents, with intracellular distribution in the cytoplasm rather than the nucleus. The results demonstrated that this beacon could directly provide quantitative measurement of the intracellular STAT5B mRNA in living cells. Compared to the previous approaches, this beacon has advantages of higher target to background ratio of detection and an increased resistance to nuclease degradation. The strategy reported in this study is a promising approach for the intracellular measurement of RNA or protein expression in living cells, and has great potential in the study of drug screening and discovery. Copyright © 2012 Elsevier B.V. All rights reserved.

Optimizing the MAC Protocol in Localization Systems Based on IEEE 802.15.4 Networks

PubMed Central

Claver, Jose M.; Ezpeleta, Santiago

2017-01-01

Radio frequency signals are commonly used in the development of indoor localization systems. The infrastructure of these systems includes some beacons placed at known positions that exchange radio packets with users to be located. When the system is implemented using wireless sensor networks, the wireless transceivers integrated in the network motes are usually based on the IEEE 802.15.4 standard. But, the CSMA-CA, which is the basis for the medium access protocols in this category of communication systems, is not suitable when several users want to exchange bursts of radio packets with the same beacon to acquire the radio signal strength indicator (RSSI) values needed in the location process. Therefore, new protocols are necessary to avoid the packet collisions that appear when multiple users try to communicate with the same beacons. On the other hand, the RSSI sampling process should be carried out very quickly because some systems cannot tolerate a large delay in the location process. This is even more important when the RSSI sampling process includes measures with different signal power levels or frequency channels. The principal objective of this work is to speed up the RSSI sampling process in indoor localization systems. To achieve this objective, the main contribution is the proposal of a new MAC protocol that eliminates the medium access contention periods and decreases the number of packet collisions to accelerate the RSSI collection process. Moreover, the protocol increases the overall network throughput taking advantage of the frequency channel diversity. The presented results show the suitability of this protocol for reducing the RSSI gathering delay and increasing the network throughput in simulated and real environments. PMID:28684666

Optimizing the MAC Protocol in Localization Systems Based on IEEE 802.15.4 Networks.

PubMed

Pérez-Solano, Juan J; Claver, Jose M; Ezpeleta, Santiago

2017-07-06

Radio frequency signals are commonly used in the development of indoor localization systems. The infrastructure of these systems includes some beacons placed at known positions that exchange radio packets with users to be located. When the system is implemented using wireless sensor networks, the wireless transceivers integrated in the network motes are usually based on the IEEE 802.15.4 standard. But, the CSMA-CA, which is the basis for the medium access protocols in this category of communication systems, is not suitable when several users want to exchange bursts of radio packets with the same beacon to acquire the radio signal strength indicator (RSSI) values needed in the location process. Therefore, new protocols are necessary to avoid the packet collisions that appear when multiple users try to communicate with the same beacons. On the other hand, the RSSI sampling process should be carried out very quickly because some systems cannot tolerate a large delay in the location process. This is even more important when the RSSI sampling process includes measures with different signal power levels or frequency channels. The principal objective of this work is to speed up the RSSI sampling process in indoor localization systems. To achieve this objective, the main contribution is the proposal of a new MAC protocol that eliminates the medium access contention periods and decreases the number of packet collisions to accelerate the RSSI collection process. Moreover, the protocol increases the overall network throughput taking advantage of the frequency channel diversity. The presented results show the suitability of this protocol for reducing the RSSI gathering delay and increasing the network throughput in simulated and real environments.

Monopulse azimuth measurement in the ATC Radar Beacon System

DOT National Transportation Integrated Search

1971-12-01

A review is made of the application of sum-difference beam : techniques to the ATC Radar Beacon System. A detailed error analysis : is presented for the case of a monopulse azimuth measurement based : on the existing beacon antenna with a modified fe...

Direct detection of microRNAs using isothermal amplification and molecular beacon with excellent sensitivity and specificity

NASA Astrophysics Data System (ADS)

Zhang, Wancun; Zhang, Qi; Qian, Zhiyu; Gu, Yueqing

2017-02-01

MicroRNAs (miRNAs) play important roles in a wide range of biological processes, including proliferation, development, metabolism, immunological response, tumorigenesis, and viral infection. The detection of miRNAs is imperative for gaining a better understanding of the functions of these biomolecules and has great potential for the early diagnosis of human disease as well as the discovery of new drugs through the use of miRNAs as targets. In this article, we develop a highly sensitive, and specific miRNA assay based on the two-stage isothermal amplification reactions and molecular beacon. The two-stage isothermal amplification reactions involves two templates and two-stage amplification reactions under isothermal conditions. The first template enables the amplification of miRNA, and the second template enables the conversion of miRNA to the reporter oligonucleotide(Y). Importantly, different miRNAs can be converted to the same Y seperately, which can hybridize with the same set of molecular beacon to generate fluorescent signals. This assay is highly sensitive and specific with a detection limit of 1 fM and can even discriminate single-nucleotide differences. Moreover, in combination with the specific templates, this method can be applied for multiplex miRNA assay by simply using the same molecular beacon. This method has potential to become a promising miRNA quantification method in biomedical research and clinical diagnosis.

14 CFR 171.21 - Scope.

Code of Federal Regulations, 2010 CFR

2010-01-01

... FACILITIES NON-FEDERAL NAVIGATION FACILITIES Nondirectional Radio Beacon Facilities § 171.21 Scope. (a) This... radio beacon facilities that are to be involved in the approval of instrument flight rules and air traffic control procedures related to those facilities. (b) A nondirectional radio beacon (“H” facilities...

State Regulation of Heliport Design

DTIC Science & Technology

2001-05-01

Arpt Lgt Sked: Dusk-Dawn 81. Schedule for beacon; if lights on different from beacon list as a remark. If no beacon list light schedule. 82. Unicorn ...continued importance of hospital heliports and the rapidly growing use of instrument approach/departure procedures at such sites, is it appropriate

Adaptive optics without altering visual perception.

PubMed

Koenig, D E; Hart, N W; Hofer, H J

2014-04-01

Adaptive optics combined with visual psychophysics creates the potential to study the relationship between visual function and the retina at the cellular scale. This potential is hampered, however, by visual interference from the wavefront-sensing beacon used during correction. For example, we have previously shown that even a dim, visible beacon can alter stimulus perception (Hofer et al., 2012). Here we describe a simple strategy employing a longer wavelength (980nm) beacon that, in conjunction with appropriate restriction on timing and placement, allowed us to perform psychophysics when dark adapted without altering visual perception. The method was verified by comparing detection and color appearance of foveally presented small spot stimuli with and without the wavefront beacon present in 5 subjects. As an important caution, we found that significant perceptual interference can occur even with a subliminal beacon when additional measures are not taken to limit exposure. Consequently, the lack of perceptual interference should be verified for a given system, and not assumed based on invisibility of the beacon. Copyright © 2014 Elsevier B.V. All rights reserved.

Accurate beacon positioning method for satellite-to-ground optical communication.

PubMed

Wang, Qiang; Tong, Ling; Yu, Siyuan; Tan, Liying; Ma, Jing

2017-12-11

In satellite laser communication systems, accurate positioning of the beacon is essential for establishing a steady laser communication link. For satellite-to-ground optical communication, the main influencing factors on the acquisition of the beacon are background noise and atmospheric turbulence. In this paper, we consider the influence of background noise and atmospheric turbulence on the beacon in satellite-to-ground optical communication, and propose a new locating algorithm for the beacon, which takes the correlation coefficient obtained by curve fitting for image data as weights. By performing a long distance laser communication experiment (11.16 km), we verified the feasibility of this method. Both simulation and experiment showed that the new algorithm can accurately obtain the position of the centroid of beacon. Furthermore, for the distortion of the light spot through atmospheric turbulence, the locating accuracy of the new algorithm was 50% higher than that of the conventional gray centroid algorithm. This new approach will be beneficial for the design of satellite-to ground optical communication systems.

Detection of DNA damage by using hairpin molecular beacon probes and graphene oxide.

PubMed

Zhou, Jie; Lu, Qian; Tong, Ying; Wei, Wei; Liu, Songqin

2012-09-15

A hairpin molecular beacon tagged with carboxyfluorescein in combination with graphene oxide as a quencher reagent was used to detect the DNA damage by chemical reagents. The fluorescence of molecular beacon was quenched sharply by graphene oxide; while in the presence of its complementary DNA the quenching efficiency decreased because their hybridization prevented the strong adsorbability of molecular beacon on graphene oxide. If the complementary DNA was damaged by a chemical reagent and could not form intact duplex structure with molecular beacon, more molecular beacon would adsorb on graphene oxide increasing the quenching efficiency. Thus, damaged DNA could be detected based on different quenching efficiencies afforded by damaged and intact complementary DNA. The damage effects of chlorpyrifos-methyl and three metabolites of styrene such as mandelieaeids, phenylglyoxylieaeids and epoxystyrene on DNA were studied as models. The method for detection of DNA damage was reliable, rapid and simple compared to the biological methods. Copyright © 2012 Elsevier B.V. All rights reserved.

Fusing Range Measurements from Ultrasonic Beacons and a Laser Range Finder for Localization of a Mobile Robot

PubMed Central

Ko, Nak Yong; Kuc, Tae-Yong

2015-01-01

This paper proposes a method for mobile robot localization in a partially unknown indoor environment. The method fuses two types of range measurements: the range from the robot to the beacons measured by ultrasonic sensors and the range from the robot to the walls surrounding the robot measured by a laser range finder (LRF). For the fusion, the unscented Kalman filter (UKF) is utilized. Because finding the Jacobian matrix is not feasible for range measurement using an LRF, UKF has an advantage in this situation over the extended KF. The locations of the beacons and range data from the beacons are available, whereas the correspondence of the range data to the beacon is not given. Therefore, the proposed method also deals with the problem of data association to determine which beacon corresponds to the given range data. The proposed approach is evaluated using different sets of design parameter values and is compared with the method that uses only an LRF or ultrasonic beacons. Comparative analysis shows that even though ultrasonic beacons are sparsely populated, have a large error and have a slow update rate, they improve the localization performance when fused with the LRF measurement. In addition, proper adjustment of the UKF design parameters is crucial for full utilization of the UKF approach for sensor fusion. This study contributes to the derivation of a UKF-based design methodology to fuse two exteroceptive measurements that are complementary to each other in localization. PMID:25970259

Quantum dot-based molecular beacon to monitor intracellular microRNAs.

PubMed

Lee, Jonghwan; Moon, Sung Ung; Lee, Yong Seung; Ali, Bahy A; Al-Khedhairy, Abdulaziz A; Ali, Daoud; Ahmed, Javed; Al Salem, Abdullah M; Kim, Soonhag

2015-06-02

Fluorescence monitoring of endogenous microRNA (miRNA or miR) activity related to neuronal development using nano-sized materials provides crucial information on miRNA expression patterns in a noninvasive manner. In this study, we report a new method to monitor intracellular miRNA124a using quantum dot-based molecular beacon (R9-QD-miR124a beacon). The R9-QD-miR124a beacon was constructed using QDs and two probes, miR124a-targeting oligomer and arginine rich cell-penetrating peptide (R9 peptide). The miR124a-targeting oligomer contains a miR124a binging sequence and a black hole quencher 1 (BHQ1). In the absence of target miR124a, the R9-QD-miR124a beacon forms a partial duplex beacon and remained in quenched state because the BHQ1 quenches the fluorescence signal of the R9-QD-miR124a beacon. The binding of miR124a to the miR124a binding sequence of the miR124a-targeting oligomer triggered the separation of the BHQ1 quencher and subsequent signal-on of a red fluorescence signal. Moreover, enhanced cellular uptake was achieved by conjugation with the R9 peptide, which resulted in increased fluorescent signal of the R9-QD-miR124a beacons in P19 cells during neurogenesis due to the endogenous expression of miR124a.

76 FR 65216 - Beacon Medical Services, LLC, Aurora, CO; Notice of Negative Determination Regarding Application...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-20

... application received July 25, 2011, a worker requested administrative reconsideration of the negative determination regarding workers' eligibility to apply for Trade Adjustment Assistance (TAA) applicable to workers and former workers of Beacon Medical Services, LLC, Aurora, Colorado (Beacon Medical Services...

46 CFR 169.744 - Emergency position indicating radio beacon (EPIRB).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Emergency position indicating radio beacon (EPIRB). 169.744 Section 169.744 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS... position indicating radio beacon (EPIRB). Each EPIRB must be marked with the vessel's name. ...

46 CFR 169.555 - Emergency position indicating radio beacon (EPIRB).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Emergency position indicating radio beacon (EPIRB). 169.555 Section 169.555 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS... Emergency position indicating radio beacon (EPIRB). (a) Each vessel certificated for exposed waters must...

Crescent Evaluation : appendix F : state-line beacon project : user case studies

DOT National Transportation Integrated Search

1994-02-01

The essence of the AVI "Beacon" concept as it applies to the HELP organization would be to provide location, date, and tune information from the roadside to the on-board computer (OBC). A roadside transmit beacon would be located on the state lines a...

Collaborating To Help High-Risk Students Succeed. Beacon Guide.

ERIC Educational Resources Information Center

Chemeketa Community Coll., Salem, OR.

The goal of the American Association of Community and Junior Colleges' Beacon Colleges Initiative is to disseminate information about exemplary collaborative programs and services. In Oregon, Chemeketa Community College is the Beacon College which has been working in association with five other community colleges in the state to build community…

46 CFR 169.831 - Emergency position indicating radio beacon (EPIRB).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Emergency position indicating radio beacon (EPIRB). 169.831 Section 169.831 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS... radio beacon (EPIRB). The master shall ensure that— (a) The EPIRB required in § 169.555 of this...

Beacon- and Schema-Based Method for Recognizing Algorithms from Students' Source Code

ERIC Educational Resources Information Center

Taherkhani, Ahmad; Malmi, Lauri

2013-01-01

In this paper, we present a method for recognizing algorithms from students programming submissions coded in Java. The method is based on the concept of "programming schemas" and "beacons". Schemas are high-level programming knowledge with detailed knowledge abstracted out, and beacons are statements that imply specific…

Beach Advisory and Closing Online Notification (BEACON) system

EPA Pesticide Factsheets

Beach Advisory and Closing Online Notification system (BEACON) is a colletion of state and local data reported to EPA about beach closings and advisories. BEACON is the public-facing query of the Program tracking, Beach Advisories, Water quality standards, and Nutrients database (PRAWN) which tracks beach closing and advisory information.

Simulation of the air traffic control radar beacon system (soar) with application to a discrete address beacon system volume II: appendixes

DOT National Transportation Integrated Search

1975-04-01

The report describes a computer simulation of the Air Traffic Control Radar Beacon System (ATCRBS). Operating on real air traffic data and actual characteristics of the relevant ground interrogators, the FORTRAN program re-enacts system operation in ...

The First Year of Beacon School Status: Maintaining Excellence and Sharing Success.

ERIC Educational Resources Information Center

Burton, Neil; Brundrett, Mark

2000-01-01

During summer 1998, the British government's Department for Education and Employment recognized 74 schools as model "beacons of excellence." A recent survey reveals that effective communication, coupled with a clear sense of purpose and vision, has enabled Beacon schools to maintain educational excellence while facilitating other…

14 CFR 99.13 - Transponder-on requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... equipped with an operable radar beacon transponder, shall operate the transponder, including altitude... designated in subpart B of this part unless that aircraft is equipped with a coded radar beacon transponder..., within, or across the contiguous U.S. ADIZ unless that aircraft is equipped with a coded radar beacon...

14 CFR 99.13 - Transponder-on requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... equipped with an operable radar beacon transponder, shall operate the transponder, including altitude... designated in subpart B of this part unless that aircraft is equipped with a coded radar beacon transponder..., within, or across the contiguous U.S. ADIZ unless that aircraft is equipped with a coded radar beacon...

54. DETAIL OF GENERAL ELECTRIC AIRBORNE BEACON EQUIPMENT TEST SET ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

54. DETAIL OF GENERAL ELECTRIC AIRBORNE BEACON EQUIPMENT TEST SET (LEFT) AND ASSOCIATED GOULD BRUSH CHART RECORDERS (RIGHT). ELAPSED TIME COUNTER SITS ATOP AIRBORNE BEACON EQUIPMENT TEST SET. - Vandenberg Air Force Base, Space Launch Complex 3, Launch Operations Building, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

14 CFR 99.13 - Transponder-on requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... equipped with an operable radar beacon transponder, shall operate the transponder, including altitude... designated in subpart B of this part unless that aircraft is equipped with a coded radar beacon transponder..., within, or across the contiguous U.S. ADIZ unless that aircraft is equipped with a coded radar beacon...

14 CFR 99.13 - Transponder-on requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... equipped with an operable radar beacon transponder, shall operate the transponder, including altitude... designated in subpart B of this part unless that aircraft is equipped with a coded radar beacon transponder..., within, or across the contiguous U.S. ADIZ unless that aircraft is equipped with a coded radar beacon...

14 CFR 99.13 - Transponder-on requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... equipped with an operable radar beacon transponder, shall operate the transponder, including altitude... designated in subpart B of this part unless that aircraft is equipped with a coded radar beacon transponder..., within, or across the contiguous U.S. ADIZ unless that aircraft is equipped with a coded radar beacon...

Simulation of the Air Traffic Control Radar Beacon System (SOAR) with application to a Discrete Address Beacon System. Volume 1 : text

DOT National Transportation Integrated Search

1975-04-01

The report describes a computer simulation of the Air Traffic Control Radar Beacon System (ATCRBS). Operating on real air traffic data and actual characteristics of the relevant ground interrogators, the FORTRAN program re-enacts system operation in ...

High efficiency low cost monolithic module for SARSAT distress beacons

NASA Technical Reports Server (NTRS)

Petersen, Wendell C.; Siu, Daniel P.

1992-01-01

The program objectives were to develop a highly efficient, low cost RF module for SARSAT beacons; achieve significantly lower battery current drain, amount of heat generated, and size of battery required; utilize MMIC technology to improve efficiency, reliability, packaging, and cost; and provide a technology database for GaAs based UHF RF circuit architectures. Presented in viewgraph form are functional block diagrams of the SARSAT distress beacon and beacon RF module as well as performance goals, schematic diagrams, predicted performances, and measured performances for the phase modulator and power amplifier.

Supporting Beacon and Event-Driven Messages in Vehicular Platoons through Token-Based Strategies

PubMed Central

Uhlemann, Elisabeth

2018-01-01

Timely and reliable inter-vehicle communications is a critical requirement to support traffic safety applications, such as vehicle platooning. Furthermore, low-delay communications allow the platoon to react quickly to unexpected events. In this scope, having a predictable and highly effective medium access control (MAC) method is of utmost importance. However, the currently available IEEE 802.11p technology is unable to adequately address these challenges. In this paper, we propose a MAC method especially adapted to platoons, able to transmit beacons within the required time constraints, but with a higher reliability level than IEEE 802.11p, while concurrently enabling efficient dissemination of event-driven messages. The protocol circulates the token within the platoon not in a round-robin fashion, but based on beacon data age, i.e., the time that has passed since the previous collection of status information, thereby automatically offering repeated beacon transmission opportunities for increased reliability. In addition, we propose three different methods for supporting event-driven messages co-existing with beacons. Analysis and simulation results in single and multi-hop scenarios showed that, by providing non-competitive channel access and frequent retransmission opportunities, our protocol can offer beacon delivery within one beacon generation interval while fulfilling the requirements on low-delay dissemination of event-driven messages for traffic safety applications. PMID:29570676

Supporting Beacon and Event-Driven Messages in Vehicular Platoons through Token-Based Strategies.

PubMed

Balador, Ali; Uhlemann, Elisabeth; Calafate, Carlos T; Cano, Juan-Carlos

2018-03-23

Timely and reliable inter-vehicle communications is a critical requirement to support traffic safety applications, such as vehicle platooning. Furthermore, low-delay communications allow the platoon to react quickly to unexpected events. In this scope, having a predictable and highly effective medium access control (MAC) method is of utmost importance. However, the currently available IEEE 802.11p technology is unable to adequately address these challenges. In this paper, we propose a MAC method especially adapted to platoons, able to transmit beacons within the required time constraints, but with a higher reliability level than IEEE 802.11p, while concurrently enabling efficient dissemination of event-driven messages. The protocol circulates the token within the platoon not in a round-robin fashion, but based on beacon data age, i.e., the time that has passed since the previous collection of status information, thereby automatically offering repeated beacon transmission opportunities for increased reliability. In addition, we propose three different methods for supporting event-driven messages co-existing with beacons. Analysis and simulation results in single and multi-hop scenarios showed that, by providing non-competitive channel access and frequent retransmission opportunities, our protocol can offer beacon delivery within one beacon generation interval while fulfilling the requirements on low-delay dissemination of event-driven messages for traffic safety applications.

Accelerated Adoption of Advanced Health Information Technology in Beacon Community Health Centers.

PubMed

Jones, Emily; Wittie, Michael

2015-01-01

To complement national and state-level HITECH Act programs, 17 Beacon communities were funded to fuel community-wide use of health information technology to improve quality. Health centers in Beacon communities received supplemental funding. This article explores the association between participation in the Beacon program and the adoption of electronic health records. Using the 2010-2012 Uniform Data System, trends in health information technology adoption among health centers located within and outside of Beacon communities were explored using differences in mean t tests and multivariate logistic regression. Electronic health record adoption was widespread and rapidly growing in all health centers, especially quality improvement functionalities: structured data capture, order and results management, and clinical decision support. Adoption lagged for functionalities supporting patient engagement, performance measurement, care coordination, and public health. The use of advanced functionalities such as care coordination grew faster in Beacon health centers, and Beacon health centers had 1.7 times higher odds of adopting health records with basic safety and quality functionalities in 2010-2012. Three factors likely underlie these findings: technical assistance, community-wide activation supporting health information exchange, and the layering of financial incentives. Additional technical assistance and community-wide activation is needed to support the use of functionalities that are currently lagging. © Copyright 2015 by the American Board of Family Medicine.

Precise locating approach of the beacon based on gray gradient segmentation interpolation in satellite optical communications.

PubMed

Wang, Qiang; Liu, Yuefei; Chen, Yiqiang; Ma, Jing; Tan, Liying; Yu, Siyuan

2017-03-01

Accurate location computation for a beacon is an important factor of the reliability of satellite optical communications. However, location precision is generally limited by the resolution of CCD. How to improve the location precision of a beacon is an important and urgent issue. In this paper, we present two precise centroid computation methods for locating a beacon in satellite optical communications. First, in terms of its characteristics, the beacon is divided into several parts according to the gray gradients. Afterward, different numbers of interpolation points and different interpolation methods are applied in the interpolation area; we calculate the centroid position after interpolation and choose the best strategy according to the algorithm. The method is called a "gradient segmentation interpolation approach," or simply, a GSI (gradient segmentation interpolation) algorithm. To take full advantage of the pixels of the beacon's central portion, we also present an improved segmentation square weighting (SSW) algorithm, whose effectiveness is verified by the simulation experiment. Finally, an experiment is established to verify GSI and SSW algorithms. The results indicate that GSI and SSW algorithms can improve locating accuracy over that calculated by a traditional gray centroid method. These approaches help to greatly improve the location precision for a beacon in satellite optical communications.

Visual detection of multidrug resistance gene in living cell using the molecular beacon imaging

NASA Astrophysics Data System (ADS)

Zhou, Qiumei; Ma, Yi; Gu, Yueqing

2014-09-01

A major problem in cancer treatment is the development of resistance to chemotherapeutic agents in tumor cells. Detection of effective prognostic biomarkers and targets are of crucial importance to the management of individualized therapies. However, quantitative analysis of the drug resistance gene had been difficult because of technical limitations. In this study, we designed and used a special hairpin deoxyribonucleic acid (DNA), which served as a beacon for detecting human drug resistance indicater. Upon hybridizing with the target mRNA, the hairpin DNA modified gold nanoparticle beacons (hDAuNP beacons) release the fluorophores attached at 5'end of the oligonucleotide sequence. The fluorescence properties of the beacon before and after the hybridization with the complementary DNA were confirmed in vitro. The hDAuNP beacons could be taken up by living cells with low inherent cytotoxicity and higher stability. hDAuNP beacon imaged by confocal laser scanning microscopy to detect the resistance gene expression. The detected fluorescence in MCF7and MCF7/ADR cells correlates with the specific drug resistance gene expression, which is consistent with the result from Q-PCR. Thus, this approach overcame many of the challenges of previous techniques by creating highly sensitive and effective intracellular probes for monitoring gene expression.

Marking emergency exits and evacuation routes with sound beacons utilizing the precedence effect

NASA Astrophysics Data System (ADS)

van Wijngaarden, Sander J.; Bronkhorst, Adelbert W.; Boer, Louis C.

2004-05-01

Sound beacons can be extremely useful during emergency evacuations, especially when vision is obscured by smoke. When exits are marked with suitable sound sources, people can find these using only their capacity for directional hearing. Unfortunately, unless very explicit instructions were given, sound beacons currently commercially available (based on modulated noise) led to disappointing results during an evacuation experiment in a traffic tunnel. Only 19% out of 65 subjects were able to find an exit by ear. A signal designed to be more self-explanatory and less hostile-sounding (alternating chime signal and spoken message ``exit here'') increased the success rate to 86%. In a more complex environment-a mock-up of a ship's interior-routes to the exit were marked using multiple beacons. By applying carefully designed time delays between successive beacons, the direction of the route was marked, utilizing the precedence effect. Out of 34 subjects, 71% correctly followed the evacuation route by ear (compared to 24% for a noise signal as used in commercially available beacons). Even when subjects were forced to make a worst-case left-right decision at a T-junction, between two beacons differing only in arrival of the first wave front, 77% made the right decision.

A quantum dot-aptamer beacon using a DNA intercalating dye as the FRET reporter: application to label-free thrombin detection.

PubMed

Chi, Chun-Wei; Lao, Yeh-Hsing; Li, Yi-Shan; Chen, Lin-Chi

2011-03-15

A new quantum dot (QD)-aptamer (apt) beacon that acts by folding-induced dissociation of a DNA intercalating dye, BOBO-3(B), is demonstrated with label-free thrombin detection. The beacon, denoted as QD-apt:B, is constructed by (1) coupling of a single-stranded thrombin aptamer to Qdot 565 via EDC/Sulfo-NHS chemistry and (2) staining the duplex regions of the aptamer on QD with excess BOBO-3 before thrombin binding. When mixing a thrombin sample with QD-apt:B, BOBO-3 is competed away from the beacon due to target-induced aptamer folding, which then causes a decrease in QD fluorescence resonance energy transfer (FRET)-mediated BOBO-3 emission and achieves thrombin quantitation. In this work, the effects of Mg(2+), coupling time, and aptamer type on the beacon's performances are investigated and discussed thoroughly with various methods, including transmission electron microscopy (TEM), dynamic light scattering (DLS), and two-color differential gel electrophoresis. Using the best aptamer beacon (HTQ37), we attain highly specific and wide-range detection (from nM to μM) of thrombin in buffer, and the beacon can sense nM-range thrombin in 15% diluted serum. Compared to the reported QD aptamer assays, our method is advantageous from the aspect of using a simple sensory unit design without losing the detection sensitivity. Therefore, we consider the QD-apt:B beacon a potential alternative to immuno-reagents and an effective tool to study nucleic acid folding on QD as well. Copyright © 2011 Elsevier B.V. All rights reserved.

Beacons In Brief. P/PV In Brief. Issue 2

ERIC Educational Resources Information Center

Blank, Susan; Farley, Chelsea

2004-01-01

This second issue in P/PV's "In Brief" series focuses on the San Francisco Beacon Initiative and P/PV's recently released evaluation results. The Beacon Initiative established after-school programs in eight public schools in low-income San Francisco neighborhoods. P/PV's 36-month evaluation examined key developmental and academic outcomes.…

47 CFR 95.1401 - Frequency.

Code of Federal Regulations, 2010 CFR

2010-10-01

... SERVICES Personal Locator Beacons (PLB). § 95.1401 Frequency. The frequency band 406.0-406.1 MHz is an emergency and distress frequency band available for use by Personal Locator Beacons (PLBs). Personal Locator Beacons that transmit on the frequency band 406.0-406.1 MHz must use G1D emission. Use of these...

What Happened to the Beacon Schools? Policy Reform and Educational Equity

ERIC Educational Resources Information Center

Smith, Emma

2015-01-01

This paper considers the impact of the Beacon schools initiative on the social and academic characteristics of secondary schools in England. The Beacon schools programme ran from 1998 to 2004 and epitomised the (then) Labour government's focus on school improvement through diversity, collaboration and partnership. This paper looks at variation in…

Highly sensitive and rapid bacteria detection using molecular beacon-Au nanoparticles hybrid nanoprobes.

PubMed

Cao, Jing; Feng, Chao; Liu, Yan; Wang, Shouyu; Liu, Fei

2014-07-15

Since many diseases are caused by pathogenic bacterial infections, accurate and rapid detection of pathogenic bacteria is in urgent need to timely apply appropriate treatments and to reduce economic costs. To end this, we designed molecular beacon-Au nanoparticle hybrid nanoprobes to improve the bacterial detection efficiency and sensitivity. Here, we show that the designed molecular beacon modified Au nanoparticles could specifically recognize synthetic DNAs targets and can readily detect targets in clinical samples. Moreover, the hybrid nanoprobes can recognize Escherichia coli within an hour at a concentration of 10(2) cfu/ml, which is 1000-folds sensitive than using molecular beacon directly. Our results show that the molecular beacon-Au nanoparticle hybrid nanoprobes have great potential in medical and biological applications. Copyright © 2014 Elsevier B.V. All rights reserved.

"Off-on" electrochemical hairpin-DNA-based genosensor for cancer diagnostics.

PubMed

Farjami, Elaheh; Clima, Lilia; Gothelf, Kurt; Ferapontova, Elena E

2011-03-01

A simple and robust "off-on" signaling genosensor platform with improved selectivity for single-nucleotide polymorphism (SNP) detection based on the electronic DNA hairpin molecular beacons has been developed. The DNA beacons were immobilized onto gold electrodes in their folded states through the alkanethiol linker at the 3'-end, while the 5'-end was labeled with a methylene blue (MB) redox probe. A typical "on-off" change of the electrochemical signal was observed upon hybridization of the 27-33 nucleotide (nt) long hairpin DNA to the target DNA, in agreement with all the hitherto published data. Truncation of the DNA hairpin beacons down to 20 nts provided improved genosensor selectivity for SNP and allowed switching of the electrochemical genosensor response from the on-off to the off-on mode. Switching was consistent with the variation in the mechanism of the electron transfer reaction between the electrode and the MB redox label, for the folded beacon being characteristic of the electrochemistry of adsorbed species, while for the "open" duplex structure being formally controlled by the diffusion of the redox label within the adsorbate layer. The relative current intensities of both processes were governed by the length of the formed DNA duplex, potential scan rate, and apparent diffusion coefficient of the redox species. The off-on genosensor design used for detection of a cancer biomarker TP53 gene sequence favored discrimination between the healthy and SNP-containing DNA sequences, which was particularly pronounced at short hybridization times.

Serum inverts and improves the fluorescence response of an aptamer beacon to various vitamin D analytes.

PubMed

Bruno, John G; Carrillo, Maria P; Phillips, Taylor; Edge, Allison

2012-01-01

A dominant aptamer loop structure from a library of nearly 100 candidate aptamer sequences developed against immobilized 25-hydroxyvitamin D(3) (calcidiol) was converted into a 5'-TYE 665 and 3'-Iowa black-labelled aptamer beacon. The aptamer beacon exhibited a mild 'lights on' reaction in buffer as a function of increasing concentrations of several vitamin D analogues and metabolites, with a limit of detection of approximately 200 ng/mL, and was not specific for any particular congener. In 10% or 50% human serum, the same aptamer beacon inverted its fluorescence behaviour to become a more intense 'lights off' reaction with an improved limit of detection in the range 4-16 ng/mL. We hypothesized that this drastic change in fluorescence behaviour was due to the presence of creatinine and urea in serum, which might destabilize the quenched beacon, causing an increase in fluorescence followed by decreasing fluorescence as a function of vitamin D concentrations that may bind and quench increasingly greater fractions of the denatured beacons. However, the results of several control experiments in the presence of physiological or greater concentrations of creatinine and urea, alone or combined in buffer, failed to produce the beacon fluorescence inversion. Other possible mechanistic hypotheses are also discussed. Copyright © 2011 John Wiley & Sons, Ltd.

An Oasis in This Desert: Parents Talk about the New York City Beacons.

ERIC Educational Resources Information Center

Nevarez, Nancy

This report presents the findings of focus groups convened to determine what the parents of youth participants in the New York City Beacons think about the program. The Beacons initiative is a comprehensive model of school-community-family partnerships undertaken by New York City in 1991. The initiative originally enabled 10 community-based…

Building Partnerships with Agencies & Employers To Help High Risk Students Succeed. The AACJC/Kellogg Beacon Colleges Initiative.

ERIC Educational Resources Information Center

American Association of Community and Junior Colleges, Washington, DC.

The American Association of Community and Junior Colleges' (AACJC's) Beacon College Project requires a Beacon College to form a consortium with five to ten associate community colleges (CCs) for the purpose of furthering the recommendations of the AACJC Futures Commission report regarding the building of communities. In Oregon, Chemeketa Community…

Bilingual Education and Academic/Career Outreach for Newcomers: Project BEACON, 1988-89. Evaluation Section Report. OREA Report.

ERIC Educational Resources Information Center

Berney, Tomi D.; Gritzer, Glenn

The Bilingual Education Academic/Career Outreach for Newcomers Program (Project BEACON) completed its fourth year. The goals of Project BEACON are to facilitate acquisition of English proficiency, develop native language literacy skills, and provide instruction in bilingual and English content area/vocational classes. The project served 753…

77 FR 41271 - Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson River, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-13

... 1625-AA00 Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson River, NY AGENCY: Coast Guard, DHS... navigable waters of the Hudson River, NY in the vicinity of Newburgh, NY for the annual Newburgh Beacon Swim... hazards associated with swimmers competing in a swim across the Hudson River. Persons and vessels are...

Beacon Editor: Capturing Signal Transduction Pathways Using the Systems Biology Graphical Notation Activity Flow Language.

PubMed

Elmarakeby, Haitham; Arefiyan, Mostafa; Myers, Elijah; Li, Song; Grene, Ruth; Heath, Lenwood S

2017-12-01

The Beacon Editor is a cross-platform desktop application for the creation and modification of signal transduction pathways using the Systems Biology Graphical Notation Activity Flow (SBGN-AF) language. Prompted by biologists' requests for enhancements, the Beacon Editor includes numerous powerful features for the benefit of creation and presentation.

Messaging with Cost-Optimized Interstellar Beacons

NASA Technical Reports Server (NTRS)

Benford, James; Benford, Gregory; Benford, Dominic

2010-01-01

On Earth, how would we build galactic-scale beacons to attract the attention of extraterrestrials, as some have suggested we should do? From the point of view of expense to a builder on Earth, experience shows an optimum trade-off. This emerges by minimizing the cost of producing a desired power density at long range, which determines the maximum range of detectability of a transmitted signal. We derive general relations for cost-optimal aperture and power. For linear dependence of capital cost on transmitter power and antenna area, minimum capital cost occurs when the cost is equally divided between antenna gain and radiated power. For nonlinear power-law dependence, a similar simple division occurs. This is validated in cost data for many systems; industry uses this cost optimum as a rule of thumb. Costs of pulsed cost-efficient transmitters are estimated from these relations by using current cost parameters ($/W, $/sq m) as a basis. We show the scaling and give examples of such beacons. Galactic-scale beacons can be built for a few billion dollars with our present technology. Such beacons have narrow "searchlight" beams and short "dwell times" when the beacon would be seen by an alien observer in their sky. More-powerful beacons are more efficient and have economies of scale: cost scales only linearly with range R, not as R(exp 2), so number of stars radiated to increases as the square of cost. On a cost basis, they will likely transmit at higher microwave frequencies, -10 GHz. The natural corridor to broadcast is along the galactic radius or along the local spiral galactic arm we are in. A companion paper asks "If someone like us were to produce a beacon, how should we look for it?"

Electroactive crown ester-Cu2+ complex with in-situ modification at molecular beacon probe serving as a facile electrochemical DNA biosensor for the detection of CaMV 35s.

PubMed

Zhan, Fengping; Liao, Xiaolei; Gao, Feng; Qiu, Weiwei; Wang, Qingxiang

2017-06-15

A novel electrochemical DNA biosensor has been facilely constructed by in-situ assembly of electroactive 4'-aminobenzo-18-crown-6-copper(II) complex (AbC-Cu 2+ ) on the free terminal of the hairpin-structured molecule beacon. The 3'-SH modified molecule beacon probe was first immobilized on the gold electrode (AuE) surface through self-assembly chemistry of Au-S bond. Then the crow ester of AbC was covalently coupled with 5'-COOH on the molecule beacon, and served as a platform to attach the Cu 2+ by coordination with ether bond (-O-) of the crown cycle. Thus, an electroactive molecule beacon-based biosensing interface was constructed. In comparison with conventional methods for preparation of electroactive molecule beacon, the approach presented in this work is much simpler, reagent- and labor-saving. Selectivity study shows that the in-situ fabricated electroactive molecule beacon remains excellent recognition ability of pristine molecule beacon probe to well differentiate various DNA fragments. The target DNA can be quantatively determined over the range from 0.10pM to 0.50nM. The detection limit of 0.060pM was estimated based on signal-to-noise ratio of 3. When the biosensor was applied for the detection cauliflower mosaic virus 35s (CaMV 35s) in soybean extraction samples, satisfactory results are achieved. This work opens a new strategy for facilely fabricating electrochemical sensing interface, which also shows great potential in aptasensor and immurosensor fabrication. Copyright © 2016 Elsevier B.V. All rights reserved.

Real-time monitoring of rolling-circle amplification using a modified molecular beacon design

PubMed Central

Nilsson, Mats; Gullberg, Mats; Dahl, Fredrik; Szuhai, Karoly; Raap, Anton K.

2002-01-01

We describe a method to monitor rolling-circle replication of circular oligonucleotides in dual-color and in real-time using molecular beacons. The method can be used to study the kinetics of the polymerization reaction and to amplify and quantify circularized oligonucleotide probes in a rolling-circle amplification (RCA) reaction. Modified molecular beacons were made of 2′-O-Me-RNA to prevent 3′ exonucleolytic degradation by the polymerase used. Moreover, the complement of one of the stem sequences of the molecular beacon was included in the RCA products to avoid fluorescence quenching due to inter-molecular hybridization of neighboring molecular beacons hybridizing to the concatemeric polymerization product. The method allows highly accurate quantification of circularized DNA over a broad concentration range by relating the signal from the test DNA circle to an internal reference DNA circle reporting in a distinct fluorescence color. PMID:12136114

Chemically directing d-block heterometallics to nanocrystal surfaces as molecular beacons of surface structure

DOE PAGES

Rosen, Evelyn L.; Gilmore, Keith; Sawvel, April M.; ...

2015-07-28

Our understanding of structure and bonding in nanoscale materials is incomplete without knowledge of their surface structure. Needed are better surveying capabilities responsive not only to different atoms at the surface, but also their respective coordination environments. We report here that d-block organometallics, when placed at nanocrystal surfaces through heterometallic bonds, serve as molecular beacons broadcasting local surface structure in atomic detail. This unique ability stems from their elemental specificity and the sensitivity of their d-orbital level alignment to local coordination environment, which can be assessed spectroscopically. Re-surfacing cadmium and lead chalcogenide nanocrystals with iron- or ruthenium-based molecular beacons ismore » readily accomplished with trimethylsilylated cyclopentadienyl metal carbonyls. For PbSe nanocrystals with iron-based beacons, we show how core-level X-ray spectroscopies and DFT calculations enrich our understanding of both charge and atomic reorganization at the surface when beacons are bound.« less

Low and Mid-Latitude Ionospheric Irregularities Studies Using TEC and Radio Scintillation Data from the CITRIS Radio Beacon Receiver in Low-Earth-Orbit

NASA Astrophysics Data System (ADS)

Siefring, C. L.; Bernhardt, P. A.; Huba, J.; Krall, J.; Roddy, P. A.

2009-12-01

Unique data on ionospheric plasma irregularities from the Naval Research Laboratory (NRL) CITRIS (Scintillation and TEC Receiver in Space) instrument will be presented. CITRIS is a multi-band receiver that recorded TEC (Total Electron Content) and radio scintillations from Low-Earth Orbit (LEO) on STPSat1. The 555+/5 km altitude 35° inclination orbit covers low and mid-latitudes. The measurements require propagation from a transmitter to a receiver through the F-region plasma. CITRIS used both 1) satellite beacons in LEO, such as the NRL CERTO (Coherent Electromagnetic Radio TOmography) beacons and 2) the global network of ground-based DORIS (Doppler Orbitography and Radiopositioning Integrated by Satellite) beacons. The TEC measurements allow for tracking of ionospheric disturbances and irregularities while the measurements of scintillations can simultaneously characterize their effects. CITRIS was operated in a complementary fashion with the C/NOFS (Communication/Navigations Outages Forecasting System) satellite during most of its first year of operations. C/NOFS carries a three-frequency 150/400/1067 MHz CERTO beacon and is dedicated to the study of Spread-F. In the case of Spread-F, ionospheric irregularities start with large scale size density gradients (100s of km) and cascade through complex processes to short scale sizes (10s of meters). It is typically the 100m-1km scale features that harm communication and navigation systems through scintillations. A multi-sensor approach is needed to completely understand this complex system, such as, the combination of CITRIS remote radio sensing and C/NOFS in-situ data. Several types of irregularities have been studied including Spread-F and the newly discovered dawn-side depletions. Comparisons with the physics based SAMI3 model are being performed to help our understanding of the morphology of the irregularities.

Evaluation of the New York City Beacons. Summary of Phase I Findings.

ERIC Educational Resources Information Center

Warren, Constancia

This report presents findings from Phase 1 of an evaluation of the New York City Beacons initiative, a model of school-community-family partnerships initiated in 1991. Beacons are community centers located in public schools, offering a range of activities and services to participants of all ages, before and after school, in the evenings, and on…

76 FR 18577 - Merritt Island National Wildlife Refuge, Volusia and Brevard Counties, FL; Collection of Entrance...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-04

... Cove, Beacon 42, and Bio Lab). Fees are not required to enter any other portion of the refuge. DATES..., Beacon 42, and Bio Lab; Black Point Wildlife Drive; public access roads; parking lots; overlooks; and... the boat ramps at Bairs Cove, Beacon 42, and Bio Lab. If public comments were to provide substantive...

Preliminary performance analysis of an interplanetary navigation system using asteroid based beacons

NASA Technical Reports Server (NTRS)

Jee, J. Rodney; Khatib, Ahmad R.; Muellerschoen, Ronald J.; Williams, Bobby G.; Vincent, Mark A.

1988-01-01

A futuristic interplanetary navigation system using transmitters placed on selected asteroids is introduced. This network of space beacons is seen as a needed alternative to the overly burdened Deep Space Network. Covariance analyses on the potential performance of these space beacons located on a candidate constellation of eight real asteroids are initiated. Simplified analytic calculations are performed to determine limiting accuracies attainable with the network for geometric positioning. More sophisticated computer simulations are also performed to determine potential accuracies using long arcs of range and Doppler data from the beacons. The results from these computations show promise for this navigation system.

Adaptive optics without altering visual perception

PubMed Central

DE, Koenig; NW, Hart; HJ, Hofer

2014-01-01

Adaptive optics combined with visual psychophysics creates the potential to study the relationship between visual function and the retina at the cellular scale. This potential is hampered, however, by visual interference from the wavefront-sensing beacon used during correction. For example, we have previously shown that even a dim, visible beacon can alter stimulus perception (Hofer, H. J., Blaschke, J., Patolia, J., & Koenig, D. E. (2012). Fixation light hue bias revisited: Implications for using adaptive optics to study color vision. Vision Research, 56, 49-56). Here we describe a simple strategy employing a longer wavelength (980nm) beacon that, in conjunction with appropriate restriction on timing and placement, allowed us to perform psychophysics when dark adapted without altering visual perception. The method was verified by comparing detection and color appearance of foveally presented small spot stimuli with and without the wavefront beacon present in 5 subjects. As an important caution, we found that significant perceptual interference can occur even with a subliminal beacon when additional measures are not taken to limit exposure. Consequently, the lack of perceptual interference should be verified for a given system, and not assumed based on invisibility of the beacon. PMID:24607992

DNA Duplex-Based Photodynamic Molecular Beacon for Targeted Killing of Retinoblastoma Cell.

PubMed

Wei, Yanchun; Lu, Cuixia; Chen, Qun; Xing, Da

2016-11-01

Retinoblastoma (RB) is the most common primary intraocular malignancy of infancy. An alternative RB treatment protocol is proposed and tested. It is based on a photodynamic therapy (PDT) with a designed molecular beacon that specifically targets the murine double minute x (MDMX) high-expressed RB cells. A MDMX mRNA triggered photodynamic molecular beacon is designed by binding a photosensitizer molecule (pyropheophorbide-a, or PPa) and a black hole quencher-3 (BHQ3) through a complementary oligonucleotide sequence. Cells with and without MDMX high-expression are incubated with the beacon and then irradiated with a laser. The fluorescence and reactive oxygen species are detected in solution to verify the specific activation of PPa by the perfectly matched DNA targets. The cell viabilities are evaluated with CCK-8 and flow cytometry assay. The fluorescence and photo-cytoxicity of PPa is recovered and significantly higher in the MDMX high-expressed Y79 and WERI-Rb1 cells, compared to that with the MDMX low-expressed cells. The synthesized beacon exhibits high PDT efficiency toward MDMX high-expressed RB cells. The data suggest that the designed beacon may provide a potential alternative for RB therapy and secures the ground for future investigation.

The Effects of Beacons, Comments, and Tasks on Program Comprehension Process in Software Maintenance

ERIC Educational Resources Information Center

Fan, Quyin

2010-01-01

Program comprehension is the most important and frequent process in software maintenance. Extensive research has found that individual characteristics of programmers, differences of computer programs, and differences of task-driven motivations are the major factors that affect the program comprehension results. There is no study specifically…

Multiple beacons for supporting lunar landing navigation

NASA Astrophysics Data System (ADS)

Theil, Stephan; Bora, Leonardo

2018-02-01

The exploration and potential future exploitation of solar system bodies requires technologies for precise and safe landings. Current navigation systems for landing probes are relying on a combination of inertial and optical sensor measurements to determine the current flight state with respect to the target body and the desired landing site. With a future transition from single exploration missions to more frequent first exploration and then exploitation missions, the implementation and operation of these missions changes, since it can be expected that a ground infrastructure on the target body is available in the vicinity of the landing site. In a previous paper, the impact of a single ground-based beacon on the navigation performance was investigated depending on the type of radiometric measurements and on the location of the beacon with respect to the landing site. This paper extends this investigation on options for ground-based multiple beacons supporting the on-board navigation system. It analyzes the impact on the achievable navigation accuracy. For that purpose, the paper introduces briefly the existing navigation architecture based on optical navigation and its extension with radiometric measurements. The same scenario of lunar landing as in the previous paper is simulated. The results are analyzed and discussed. They show a single beacon at a large distance along the landing trajectory and multiple beacons close to the landing site can improve the navigation performance. The results show how large the landing area can be increased where a sufficient navigation performance is achieved using the beacons.

CHARACTERIZATION OF FINE PARTICULATE MATTER

EPA Science Inventory

Size distribution data processing and fitting
Ultrafine, very fine and fine PM were collected nearly continuously from December 2000 through March 2003 at a Washington State Department of Ecology site on Beacon Hill in Seattle. Particle size distributio...

Beacon data acquisition and display system

DOEpatents

Skogmo, D.G.; Black, B.D.

1991-12-17

A system for transmitting aircraft beacon information received by a secondary surveillance radar through telephone lines to a remote display includes a digitizer connected to the radar for preparing a serial file of data records containing position and identification information of the beacons detected by each sweep of the radar. This information is transmitted through the telephone lines to a remote computer where it is displayed. 6 figures.

Beacon data acquisition and display system

DOEpatents

Skogmo, David G.; Black, Billy D.

1991-01-01

A system for transmitting aircraft beacon information received by a secondary surveillance radar through telephone lines to a remote display includes a digitizer connected to the radar for preparing a serial file of data records containing position and identification information of the beacons detected by each sweep of the radar. This information is transmitted through the telephone lines to a remote computer where it is displayed.

33 CFR 149.535 - What are the requirements for rotating beacons on platforms?

Code of Federal Regulations, 2011 CFR

2011-07-01

... beacon must: (a) Have an effective intensity of at least 15,000 candela; (b) Flash at least once every 20 seconds; (c) Provide a white light signal; (d) Operate in wind speeds of up to 100 knots at a rotation rate that is within 6 percent of the operating speed displayed on the beacon; (e) Have one or more...

33 CFR 149.535 - What are the requirements for rotating beacons on platforms?

Code of Federal Regulations, 2013 CFR

2013-07-01

... beacon must: (a) Have an effective intensity of at least 15,000 candela; (b) Flash at least once every 20 seconds; (c) Provide a white light signal; (d) Operate in wind speeds of up to 100 knots at a rotation rate that is within 6 percent of the operating speed displayed on the beacon; (e) Have one or more...

33 CFR 149.535 - What are the requirements for rotating beacons on platforms?

Code of Federal Regulations, 2012 CFR

2012-07-01

... beacon must: (a) Have an effective intensity of at least 15,000 candela; (b) Flash at least once every 20 seconds; (c) Provide a white light signal; (d) Operate in wind speeds of up to 100 knots at a rotation rate that is within 6 percent of the operating speed displayed on the beacon; (e) Have one or more...

33 CFR 149.535 - What are the requirements for rotating beacons on platforms?

Code of Federal Regulations, 2010 CFR

2010-07-01

... beacon must: (a) Have an effective intensity of at least 15,000 candela; (b) Flash at least once every 20 seconds; (c) Provide a white light signal; (d) Operate in wind speeds of up to 100 knots at a rotation rate that is within 6 percent of the operating speed displayed on the beacon; (e) Have one or more...

33 CFR 149.535 - What are the requirements for rotating beacons on platforms?

Code of Federal Regulations, 2014 CFR

2014-07-01

... beacon must: (a) Have an effective intensity of at least 15,000 candela; (b) Flash at least once every 20 seconds; (c) Provide a white light signal; (d) Operate in wind speeds of up to 100 knots at a rotation rate that is within 6 percent of the operating speed displayed on the beacon; (e) Have one or more...

SU-F-J-142: Proposed Method to Broaden Inclusion Potential of Patients Able to Use the Calypso Tracking System in Prostate Radiotherapy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fiedler, D; Kuo, H; Bodner, W

2016-06-15

Purpose: To introduce a non-standard method of patient setup, using BellyBoard immobilization, to better utilize the localization and tracking potential of an RF-beacon system with EBRT for prostate cancer. Methods: An RF-beacon phantom was imaged using a wide bore CT scanner, both in a standard level position and with a known rotation (4° pitch and 7.5° yaw). A commercial treatment planning system (TPS) was used to determine positional coordinates of each beacon, and the centroid of the three beacons for both setups. For each setup at the Linac, kV AP and Rt Lateral images were obtained. A full characterization ofmore » the RF-beacon system in clinical mode was completed for various beacons’ array-to-centroid distances, which includes vertical, lateral, and longitudinal offset data, as well as pitch and yaw offset measurements for the tilted phantom. For the single patient who has been setup using the proposed BellyBoard method, a supine simulation was first obtained. When abdominal protrusion was found to be exceeding the limits of the RF-Beacon system through distance-based analysis in the TPS, the patient is re-simulated prone with the BellyBoard. Array to centroid distance is measured again in the TPS, and if found to be within the localization or tracking region it is applied. Results: Characterization of limitations for the RF-beacon system in clinical mode showed acceptable consistency of offset determination for phantom setup accuracy. The nonstandard patient setup method reduced the beacons’ centroid-to-array distance by 8.32cm, from 25.13cm to 16.81cm; completely out of tracking range (greater than 20cm) to within setup tracking range (less than 20cm). Conclusion: Using the RF-beacon system in combination with this novel patient setup can allow patients who would otherwise not be candidates for beacon enhanced EBRT to now be able to benefit from the reduced PTV margins of this treatment method.« less

Per-Pixel, Dual-Counter Scheme for Optical Communications

NASA Technical Reports Server (NTRS)

Farr, William H.; Bimbaum, Kevin M.; Quirk, Kevin J.; Sburlan, Suzana; Sahasrabudhe, Adit

2013-01-01

Free space optical communications links from deep space are projected to fulfill future NASA communication requirements for 2020 and beyond. Accurate laser-beam pointing is required to achieve high data rates at low power levels.This innovation is a per-pixel processing scheme using a pair of three-state digital counters to implement acquisition and tracking of a dim laser beacon transmitted from Earth for pointing control of an interplanetary optical communications system using a focal plane array of single sensitive detectors. It shows how to implement dim beacon acquisition and tracking for an interplanetary optical transceiver with a method that is suitable for both achieving theoretical performance, as well as supporting additional functions of high data rate forward links and precision spacecraft ranging.

Beaconing Signalization Substantially Reduces Blind Pedestrians’ Veer on Snow-Covered Pavement

PubMed Central

Guth, David A.; Long, Richard G.; Kim, Dae Shik; Robertson, Elizabeth A.; Reesor, Abbie L.; Bacik, Catherine J.; Eckert, Jaclyn M.

2017-01-01

Veering outside of crosswalks is a common problem experienced by individuals who are blind. One technology found to be effective for reducing this veer when other guidance cues are absent is audible beaconing. However, veering in general and veering from crosswalks in particular have been studied primarily on smooth, flat walking surfaces such as clear pavement. This experiment compared veering on clear pavement with veering on snow-covered pavement, with and without audible beaconing. Eleven blind participants traveling with long canes attempted to walk a straight path for 72 ft (21.9 m), a typical length of a six-lane crosswalk. Beaconing substantially reduced veering at 36 ft (11.0 m) and 72 ft from the starting point and enabled participants to remain within a simulated crosswalk. Walking on snow was not found to affect veering but did increase the number of steps taken. The findings suggest that in snowy and clear conditions alike, audible beaconing is an effective wayfinding tool for intersections equipped with accessible pedestrian signals. PMID:29307955

Measuring Time-of-Flight in an Ultrasonic LPS System Using Generalized Cross-Correlation

PubMed Central

Villladangos, José Manuel; Ureña, Jesús; García, Juan Jesús; Mazo, Manuel; Hernández, Álvaro; Jiménez, Ana; Ruíz, Daniel; De Marziani, Carlos

2011-01-01

In this article, a time-of-flight detection technique in the frequency domain is described for an ultrasonic Local Positioning System (LPS) based on encoded beacons. Beacon transmissions have been synchronized and become simultaneous by means of the DS-CDMA (Direct-Sequence Code Division Multiple Access) technique. Every beacon has been associated to a 255-bit Kasami code. The detection of signal arrival instant at the receiver, from which the distance to each beacon can be obtained, is based on the application of the Generalized Cross-Correlation (GCC), by using the cross-spectral density between the received signal and the sequence to be detected. Prior filtering to enhance the frequency components around the carrier frequency (40 kHz) has improved estimations when obtaining the correlation function maximum, which implies an improvement in distance measurement precision. Positioning has been achieved by using hyperbolic trilateration, based on the Time Differences of Arrival (TDOA) between a reference beacon and the others. PMID:22346645

Measuring time-of-flight in an ultrasonic LPS system using generalized cross-correlation.

PubMed

Villladangos, José Manuel; Ureña, Jesús; García, Juan Jesús; Mazo, Manuel; Hernández, Alvaro; Jiménez, Ana; Ruíz, Daniel; De Marziani, Carlos

2011-01-01

In this article, a time-of-flight detection technique in the frequency domain is described for an ultrasonic local positioning system (LPS) based on encoded beacons. Beacon transmissions have been synchronized and become simultaneous by means of the DS-CDMA (direct-sequence code Division multiple access) technique. Every beacon has been associated to a 255-bit Kasami code. The detection of signal arrival instant at the receiver, from which the distance to each beacon can be obtained, is based on the application of the generalized cross-correlation (GCC), by using the cross-spectral density between the received signal and the sequence to be detected. Prior filtering to enhance the frequency components around the carrier frequency (40 kHz) has improved estimations when obtaining the correlation function maximum, which implies an improvement in distance measurement precision. Positioning has been achieved by using hyperbolic trilateration, based on the time differences of arrival (TDOA) between a reference beacon and the others.

Improving the detectability of oxygen saturation level targets for preterm neonates: A laboratory test of tremolo and beacon sonifications.

PubMed

Deschamps, Marie-Lys; Sanderson, Penelope; Hinckfuss, Kelly; Browning, Caitlin; Loeb, Robert G; Liley, Helen; Liu, David

2016-09-01

Recent guidelines recommend oxygen saturation (SpO2) levels of 90%-95% for preterm neonates on supplemental oxygen but it is difficult to discern such levels with current pulse oximetry sonifications. We tested (1) whether adding levels of tremolo to a conventional log-linear pulse oximetry sonification would improve identification of SpO2 ranges, and (2) whether adding a beacon reference tone to conventional pulse oximetry confuses listeners about the direction of change. Participants using the Tremolo (94%) or Beacon (81%) sonifications identified SpO2 range significantly more accurately than participants using the LogLinear sonification (52%). The Beacon sonification did not confuse participants about direction of change. The Tremolo sonification may have advantages over the Beacon sonification for monitoring SpO2 of preterm neonates, but both must be further tested with clinicians in clinically representative scenarios, and with different levels of ambient noise and distractions. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

Beaconing Signalization Substantially Reduces Blind Pedestrians' Veer on Snow-Covered Pavement.

PubMed

Guth, David A; Long, Richard G; Kim, Dae Shik; Robertson, Elizabeth A; Reesor, Abbie L; Bacik, Catherine J; Eckert, Jaclyn M

2017-01-01

Veering outside of crosswalks is a common problem experienced by individuals who are blind. One technology found to be effective for reducing this veer when other guidance cues are absent is audible beaconing. However, veering in general and veering from crosswalks in particular have been studied primarily on smooth, flat walking surfaces such as clear pavement. This experiment compared veering on clear pavement with veering on snow-covered pavement, with and without audible beaconing. Eleven blind participants traveling with long canes attempted to walk a straight path for 72 ft (21.9 m), a typical length of a six-lane crosswalk. Beaconing substantially reduced veering at 36 ft (11.0 m) and 72 ft from the starting point and enabled participants to remain within a simulated crosswalk. Walking on snow was not found to affect veering but did increase the number of steps taken. The findings suggest that in snowy and clear conditions alike, audible beaconing is an effective wayfinding tool for intersections equipped with accessible pedestrian signals.

The 406 MHz ELT/EPIRBs. [Emergency Locator Transmitters/Emergency Position Indicating Radio Beacons (ELT/EPIRB)

NASA Technical Reports Server (NTRS)

Flatow, F. S.; Gal, C.; Hayes, E. J.

1984-01-01

Specifications for the COSPAS/SARSAT beacons are presented and related design considerations are discussed. Critical design aspects having significant impact on cost and performance are highlighted. Among these is the oscillator, whose frequency drift specifications require stabilization by ovens or digital control. Design options are presented and their impact on cost and performance assessed. Beacon designs developed to meet COSPAS/SARSAT specifications are shown.

The Development of Landmark and Beacon Use in Young Children: Evidence from a Touchscreen Search Task

ERIC Educational Resources Information Center

Sutton, Jennifer E.

2006-01-01

Children ages 2, 3 and 4 years participated in a novel hide-and-seek search task presented on a touchscreen monitor. On beacon trials, the target hiding place could be located using a beacon cue, but on landmark trials, searching required the use of a nearby landmark cue. In Experiment 1, 2-year-olds performed less accurately than older children…

Energy-Efficient BOP-Based Beacon Transmission Scheduling in Wireless Sensor Networks

NASA Astrophysics Data System (ADS)

Kim, Eui-Jik; Youm, Sungkwan; Choi, Hyo-Hyun

Many applications in wireless sensor networks (WSNs) require the energy efficiency and scalability. Although IEEE 802.15.4/Zigbee which is being considered as general technology for WSNs enables the low duty-cycling with time synchronization of all the nodes in network, it still suffer from its low scalability due to the beacon frame collision. Recently, various algorithms to resolve this problem are proposed. However, their manners to implement are somewhat ambiguous and the degradation of energy/communication efficiency is serious by the additional overhead. This paper describes an Energy-efficient BOP-based Beacon transmission Scheduling (EBBS) algorithm. EBBS is the centralized approach, in which a resource-sufficient node called as Topology Management Center (TMC) allocates the time slots to transmit a beacon frame to the nodes and manages the active/sleep schedules of them. We also propose EBBS with Adaptive BOPL (EBBS-AB), to adjust the duration to transmit beacon frames in every beacon interval, adaptively. Simulation results show that by using the proposed algorithm, the energy efficiency and the throughput of whole network can be significantly improved. EBBS-AB is also more effective for the network performance when the nodes are uniformly deployed on the sensor field rather than the case of random topologies.

Quencher-free molecular beacon tethering 7-hydroxycoumarin detects targets through protonation/deprotonation.

PubMed

Kashida, Hiromu; Yamaguchi, Kyohei; Hara, Yuichi; Asanuma, Hiroyuki

2012-07-15

In this study, we synthesized a simple but efficient quencher-free molecular beacon tethering 7-hydroxycoumarin on D-threoninol based on its pK(a) change. The pK(a) of 7-hydroxycoumarin in a single strand was determined as 8.8, whereas that intercalated in the duplex was over 10. This large pK(a) shift (more than 1.2) upon hybridization could be attributed to the anionic and hydrophobic microenvironment inside the DNA duplex. Because 7-hydroxycoumarin quenches its fluorescence upon protonation, the emission intensity of the duplex at pH 8.5 was 1/15 that of the single strand. We applied this quenching mechanism to the preparation of a quencher-free molecular beacon by introducing the dye into the middle of the stem part. In the absence of the target, the stem region formed a duplex and fluorescence was quenched. However, when the target was added, the molecular beacon opened and the dye was deprotonated. As a result, the emission intensity of the molecular beacon with the target was 10 times higher than that without the target. Accordingly, a quencher-free molecular beacon utilizing the pK(a) change was successfully developed. Copyright © 2012 Elsevier Ltd. All rights reserved.

MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA.

PubMed

James, Amanda Marie; Baker, Meredith B; Bao, Gang; Searles, Charles D

2017-01-01

MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer, and cardiovascular disease (CVD). Commonly, miRNA abundance is assessed using quantitative real-time PCR (qRT-PCR), however, qRT-PCR for miRNA can be labor intensive, time consuming, and may lack specificity for detection of mature versus precursor forms of miRNA. Here, we describe a novel double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for disease. Using this approach, we found that molecular beacons with DNA or combined locked nucleic acid (LNA)-DNA backbones can detect mature and precursor miRNAs (pre-miRNAs) of low (< 1 nM) abundance in vitro . The double molecular beacon assay was accurate in assessing miRNA abundance in a sample containing a mixed population of mature and precursor miRNAs. In contrast, qRT-PCR and the single molecular beacon assay overestimated miRNA abundance. Additionally, the double molecular beacon assay was less labor intensive than traditional qRT-PCR and had 10-25% increased specificity. Our data suggest that the double molecular beacon-based approach is more precise and specific than previous methods, and has the promise of being the standard for assessing miRNA levels in biological samples.

MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA

PubMed Central

James, Amanda Marie; Baker, Meredith B.; Bao, Gang; Searles, Charles D.

2017-01-01

MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer, and cardiovascular disease (CVD). Commonly, miRNA abundance is assessed using quantitative real-time PCR (qRT-PCR), however, qRT-PCR for miRNA can be labor intensive, time consuming, and may lack specificity for detection of mature versus precursor forms of miRNA. Here, we describe a novel double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for disease. Using this approach, we found that molecular beacons with DNA or combined locked nucleic acid (LNA)-DNA backbones can detect mature and precursor miRNAs (pre-miRNAs) of low (< 1 nM) abundance in vitro. The double molecular beacon assay was accurate in assessing miRNA abundance in a sample containing a mixed population of mature and precursor miRNAs. In contrast, qRT-PCR and the single molecular beacon assay overestimated miRNA abundance. Additionally, the double molecular beacon assay was less labor intensive than traditional qRT-PCR and had 10-25% increased specificity. Our data suggest that the double molecular beacon-based approach is more precise and specific than previous methods, and has the promise of being the standard for assessing miRNA levels in biological samples. PMID:28255356

BEACON: automated tool for Bacterial GEnome Annotation ComparisON.

PubMed

Kalkatawi, Manal; Alam, Intikhab; Bajic, Vladimir B

2015-08-18

Genome annotation is one way of summarizing the existing knowledge about genomic characteristics of an organism. There has been an increased interest during the last several decades in computer-based structural and functional genome annotation. Many methods for this purpose have been developed for eukaryotes and prokaryotes. Our study focuses on comparison of functional annotations of prokaryotic genomes. To the best of our knowledge there is no fully automated system for detailed comparison of functional genome annotations generated by different annotation methods (AMs). The presence of many AMs and development of new ones introduce needs to: a/ compare different annotations for a single genome, and b/ generate annotation by combining individual ones. To address these issues we developed an Automated Tool for Bacterial GEnome Annotation ComparisON (BEACON) that benefits both AM developers and annotation analysers. BEACON provides detailed comparison of gene function annotations of prokaryotic genomes obtained by different AMs and generates extended annotations through combination of individual ones. For the illustration of BEACON's utility, we provide a comparison analysis of multiple different annotations generated for four genomes and show on these examples that the extended annotation can increase the number of genes annotated by putative functions up to 27%, while the number of genes without any function assignment is reduced. We developed BEACON, a fast tool for an automated and a systematic comparison of different annotations of single genomes. The extended annotation assigns putative functions to many genes with unknown functions. BEACON is available under GNU General Public License version 3.0 and is accessible at: http://www.cbrc.kaust.edu.sa/BEACON/ .

Prototyping and testing of a fully autonomous road construction beacon, the iCone.

DOT National Transportation Integrated Search

2010-04-01

A revolutionary portable traffic monitoring device is developed, extensively prototyped and thoroughly tested throughout the State of New York as well as several other states. The resulting device, trademarked as the iCone, simplifies the process o...

Facilitative Strategies in Action.

ERIC Educational Resources Information Center

Fuller, Thara M. A.; Haugabrook, Adrian K.

2001-01-01

Describes campus-based strategies to facilitate collaboration by examining the process of restructuring a division of student affairs as an educational partner with academic affairs. Describes three collaborative efforts at the University of Massachusetts Boston: the Beacon Leadership Project, the Diversity Research Initiative, and the Beacon…

Traveling-Wave Tube Amplifier Second Harmonic as Millimeter-Wave Beacon Source for Atmospheric Propagation Studies

NASA Technical Reports Server (NTRS)

Simons, Rainee N.; Wintucky, Edwin G.

2014-01-01

This paper presents the design and test results of a CW millimeter-wave satellite beacon source, based on the second harmonic from a traveling-wave tube amplifier and utilizes a novel waveguide multimode directional coupler. A potential application of the beacon source is for investigating the atmospheric effects on Q-band (37 to 42 GHz) and V/W-band (71 to 76 GHz) satellite-to-ground signals.

Traveling-Wave Tube Amplifier Second Harmonic as Millimeter-Wave Beacon Source for Atmospheric Propagation Studies

NASA Technical Reports Server (NTRS)

Simons, Rainee N.; Wintucky, Edwin G.

2014-01-01

This paper presents the design and test results of a CW millimeter-wave satellite beacon source, based on the second harmonic from a traveling-wave tube amplifier and utilizes a novel waveguide multimode directional coupler. A potential application of the beacon source is for investigating the atmospheric effects on Q-band (37-42 GHz) and V/W-band (71- 76 GHz) satellite-to-ground signals.

Olympus propagation studies in the US: Propagation terminal hardware and experiments

NASA Technical Reports Server (NTRS)

Stutzmzn, Warren L.

1990-01-01

Virginia Tech is performing a comprehensive set of propagation measurements using the Olympus satellite beacons at 12.5, 20, and 30 GHz. These data will be used to characterize propagation conditions on small earth terminal (VSAT)-type networks for next generation small aperture Ka-band systems. The European Space Agency (ESA) satellite Olympus was launched July 12, 1989. The spacecraft contains a sophisticated package of propagation beacons operating at 12.5, 19.77, and 29.66 GHz (referred to as 12.5, 20, and 30 beacons). These beacons cover the east coast of the United States with sufficient power for attenuation measurements. The Virginia Satellite Communications Group is completing the hardware construction phase and will begin formal data collection in June.

Precise Selenodetic Coordinate System on Artificial Light Refers

NASA Astrophysics Data System (ADS)

Bagrov, Alexander; Pichkhadze, Konstantin M.; Sysoev, Valentin

Historically a coordinate system for the Moon was established on the base of telescopic observations from the Earth. As the angular resolution of Earth-to-Space telescopic observations is limited by Earth atmosphere, and is ordinary worse then 1 ang. second, the mean accuracy of selenodetic coordinates is some angular minutes, which corresponds to errors about 900 meters for positions of lunar objects near center of visible lunar disk, and at least twice more when objects are near lunar poles. As there are no Global Positioning System nor any astronomical observation instruments on the Moon, we proposed to use an autonomous light beacon on the Luna-Globe landing module to fix its position on the surface of the moon ant to use it as refer point for fixation of spherical coordinates system for the Moon. The light beacon is designed to be surely visible by orbiting probe TV-camera. As any space probe has its own stars-orientation system, there is not a problem to calculate a set of directions to the beacon and to the referent stars in probe-centered coordinate system during flight over the beacon. Large number of measured angular positions and time of each observation will be enough to calculate both orbital parameters of the probe and selenodetic coordinates of the beacon by methods of geodesy. All this will allow fixing angular coordinates of any feature of lunar surface in one global coordinate system, referred to the beacon. The satellite’s orbit plane contains ever the center mass of main body, so if the beacon will be placed closely to a lunar pole, we shall determine pole point position of the Moon with accuracy tens times better then it is known now. When angular accuracy of self-orientation by stars of the orbital module of Luna-Glob mission will be 6 angular seconds, then being in circular orbit with height of 200 km the on-board TV-camera will allow calculation of the beacon position as well as 6" corresponding to spatial resolution of the camera. It mean that coordinates of the beacon will be determined with accuracy not worse then 6 meters on the lunar surface. Much more accuracy can be achieved if orbital probe will use as precise angular measurer as optical interferometer. The limiting accuracy of proposed method is far above any reasonable level, because it may be sub-millimeter one. Theoretical analysis shows that for achievement of 1-meter accuracy of coordinate measuring over lunar globe it will be enough to disperse over it surface some 60 light beacons. Designed by Lavochkin Association light beacon is autonomous one, and it will work at least 10 years, so coordinate frame of any other lunar mission could use established selenodetic coordinates during this period. The same approach may be used for establishing Martial coordinates system.

Rapid detection of rifampin resistance in Mycobacterium tuberculosis isolates from India and Mexico by a molecular beacon assay.

PubMed

Varma-Basil, Mandira; El-Hajj, Hiyam; Colangeli, Roberto; Hazbón, Manzour Hernando; Kumar, Sujeet; Bose, Mridula; Bobadilla-del-Valle, Miriam; García, Lourdes García; Hernández, Araceli; Kramer, Fred Russell; Osornio, Jose Sifuentes; Ponce-de-León, Alfredo; Alland, David

2004-12-01

We assessed the performance of a rapid, single-well, real-time PCR assay for the detection of rifampin-resistant Mycobacterium tuberculosis by using clinical isolates from north India and Mexico, regions with a high incidence of tuberculosis. The assay uses five differently colored molecular beacons to determine if a short region of the M. tuberculosis rpoB gene contains mutations that predict rifampin resistance in most isolates. Until now, the assay had not been sufficiently tested on samples from countries with a high incidence of tuberculosis. In the present study, the assay detected mutations in 16 out of 16 rifampin-resistant isolates from north India (100%) and in 55 of 64 rifampin-resistant isolates from Mexico (86%) compared to results with standard susceptibility testing. The assay did not detect mutations (a finding predictive of rifampin susceptibility) in 37 out of 37 rifampin-susceptible isolates from India (100%) and 125 out of 126 rifampin-susceptible isolates from Mexico (99%). DNA sequencing revealed that none of the nine rifampin-resistant isolates from Mexico, which were misidentified as rifampin susceptible by the molecular beacon assay, contained a mutation in the region targeted by the molecular beacons. The one rifampin-susceptible isolate from Mexico that appeared to be rifampin resistant by the molecular beacon assay contained an S531W mutation, which is usually associated with rifampin resistance. Of the rifampin-resistant isolates that were correctly identified in the molecular beacon assay, one contained a novel L530A mutation and another contained a novel deletion between codons 511 and 514. Overall, the molecular beacon assay appears to have sufficient sensitivity (89%) and specificity (99%) for use in countries with a high prevalence of tuberculosis.

Development of an aptamer beacon for detection of interferon-gamma.

PubMed

Tuleuova, Nazgul; Jones, Caroline N; Yan, Jun; Ramanculov, Erlan; Yokobayashi, Yohei; Revzin, Alexander

2010-03-01

Traditional antibody-based affinity sensing strategies employ multiple reagents and washing steps and are unsuitable for real-time detection of analyte binding. Aptamers, on the other hand, may be designed to monitor binding events directly, in real-time, without the need for secondary labels. The goal of the present study was to design an aptamer beacon for fluorescence resonance energy transfer (FRET)-based detection of interferon-gamma (IFN-gamma)--an important inflammatory cytokine. Variants of DNA aptamer modified with biotin moieties and spacers were immobilized on avidin-coated surfaces and characterized by surface plasmon resonance (SPR). The SPR studies showed that immobilization of aptamer via the 3' end resulted in the best binding IFN-gamma (K(d) = 3.44 nM). This optimal aptamer variant was then used to construct a beacon by hybridizing fluorophore-labeled aptamer with an antisense oligonucleotide strand carrying a quencher. SPR studies revealed that IFN-gamma binding with an aptamer beacon occurred within 15 min of analyte introduction--suggesting dynamic replacement of the quencher-complementary strand by IFN-gamma molecules. To further highlight biosensing applications, aptamer beacon molecules were immobilized inside microfluidic channels and challenged with varying concentration of analyte. Fluorescence microscopy revealed low fluorescence in the absence of analyte and high fluorescence after introduction of IFN-gamma. Importantly, unlike traditional antibody-based immunoassays, the signal was observed directly upon binding of analyte without the need for multiple washing steps. The surface immobilized aptamer beacon had a linear range from 5 to 100 nM and a lower limit of detection of 5 nM IFN-gamma. In conclusion, we designed a FRET-based aptamer beacon for monitoring of an inflammatory cytokine-IFN-gamma. In the future, this biosensing strategy will be employed to monitor dynamics of cytokine production by the immune cells.

Beacon communities' public health initiatives: a case study analysis.

PubMed

Massoudi, Barbara L; Marcial, Laura H; Haque, Saira; Bailey, Robert; Chester, Kelley; Cunningham, Shellery; Riley, Amanda; Soper, Paula

2014-01-01

The Beacon Communities for Public Health (BCPH) project was launched in 2011 to gain a better understanding of the range of activities currently being conducted in population- and public health by the Beacon Communities. The project highlighted the successes and challenges of these efforts with the aim of sharing this information broadly among the public health community. The Beacon Community Program, designed to showcase technology-enabled, community-based initiatives to improve outcomes, focused on: building and strengthening health information technology (IT) infrastructure and exchange capabilities; translating investments in health IT to measureable improvements in cost, quality, and population health; and, developing innovative approaches to performance measurement, technology, and care delivery. Four multimethod case studies were conducted based on a modified sociotechnical framework to learn more about public health initiative implementation and use in the Beacon Communities. Our methodological approach included using document review and semistructured key informant interviews. NACCHO Model Practice Program criteria were used to select the public health initiatives included in the case studies. Despite differences among the case studies, common barriers and facilitators were found to be present in all areas of the sociotechnical framework application including structure, people, technology, tasks, overarching considerations, and sustainability. Overall, there were many more facilitators (range = 7-14) present for each Beacon compared to barriers (range = 4-6). Four influential promising practices were identified through the work: forging strong and sustainable partnerships; ensuring a good task-technology fit and a flexible and iterative design; fostering technology acceptance; and, providing education and demonstrating value. A common weakness was the lack of a framework or model for the Beacon Communities evaluation work. Sharing a framework or approach to evaluation at the beginning of implementation made the work more effective. Supporting evaluation to inform future implementations is important.

Microcomputer system for receiving and processing of satellite TOVS/TIP data for vertical sounding of the atmosphere

NASA Astrophysics Data System (ADS)

Baranski, L. A.; Rozemski, K.

TOVS/TIP digital data transmitted at the VHF-BEACON range from NOAA satellites are receiving and processing at the SDRPC. Receiving station is connected with the microcomputer IBM-PC/AT which process TOVS/TIP data via two states: initial data processing and retrieval of vertical profiles of the temperature, water vapour and ozone mixing ratio in the atmosphere. Receiving and processing equipment, retrieval methods, results and error discussion are presented.

Traveling-Wave Tube Amplifier Second Harmonic as Millimeter-Wave Beacon Source for Atmospheric Propagation Studies

NASA Technical Reports Server (NTRS)

Simons, Rainee N.; Wintucky, Edwin G.

2014-01-01

The design and test results of a novel waveguide multimode directional coupler for a CW millimeter-wave satellite beacon source are presented. The coupler separates the second harmonic power from the fundamental output power of a traveling-wave tube amplifier. A potential application of the beacon source is for investigating the atmospheric effects on Q-band (37 to 42 GHz) and VW-band (71 to 76 GHz) satellite-to-ground signals.

Laser Transmitter Aims At Laser Beacon

NASA Technical Reports Server (NTRS)

Hemmati, Hamid; Lesh, James R.

1993-01-01

Transmitter part of developmental optical communication system. Compact, lightweight, partially-self-aiming laser transmitter built to verify some capabilities of developmental free-space optical communication system. Design capable of providing 0.5 Mbps data return over range equal to Moon-Earth distance. Breadboard of transmitting terminal constructed and tested in laboratory. Prototype transmitter includes receiving circuitry that keeps it aimed at beacon, once brought into initial alignment within about 1.7 degrees of line of sight to beacon.

A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction

PubMed Central

Szuhai, Károly; Sandhaus, Emily; Kolkman-Uljee, Sandra M.; Lemaître, Marc; Truffert, Jean-Christophe; Dirks, Roeland W.; Tanke, Hans J.; Fleuren, Gert Jan; Schuuring, Ed; Raap, Anton K.

2001-01-01

Human papillomaviruses (HPVs) play an important role in the pathogenesis of cervical cancer. For identification of the large number of different HPV types found in (pre)malignant lesions, a robust methodology is needed that combines general HPV detection with HPV genotyping. We have developed for formaldehyde-fixed samples a strategy that, in a homogenous, real-time fluorescence polymerase chain reaction (PCR)-based assay, accomplishes general HPV detection by SybrGreen reporting of HPV-DNA amplicons, and genotyping of seven prevalent HPV types (HPV-6, -11, -16, -18, -31, -33, -45) by real-time molecular beacon PCR. The false-positive rate of the HPV SybrGreen-PCR was 4%, making it well suited as a prescreening, general HPV detection technology. The type specificity of the seven selected HPV molecular beacons was 100% and double infections were readily identified. The multiplexing capacity of the HPV molecular beacon PCR was analyzed and up to three differently labeled molecular beacons could be used in one PCR reaction without observing cross talk. The inherent quantitation capacities of real-time fluorescence PCR allowed the determination of average HPV copy number per cell. We conclude that the HPV SybrGreen-PCR in combination with the HPV molecular beacon PCR provides a robust, sensitive, and quantitative general HPV detection and genotyping methodology. PMID:11696426

A nonlinear OPC technique for laser beam control in turbulent atmosphere

NASA Astrophysics Data System (ADS)

Markov, V.; Khizhnyak, A.; Sprangle, P.; Ting, A.; DeSandre, L.; Hafizi, B.

2013-05-01

A viable beam control technique is critical for effective laser beam transmission through turbulent atmosphere. Most of the established approaches require information on the impact of perturbations on wavefront propagated waves. Such information can be acquired by measuring the characteristics of the target-scattered light arriving from a small, preferably diffraction-limited, beacon. This paper discusses an innovative beam control approach that can support formation of a tight laser beacon in deep turbulence conditions. The technique employs Brillouin enhanced fourwave mixing (BEFWM) to generate a localized beacon spot on a remote image-resolved target. Formation of the tight beacon doesn't require a wavefront sensor, AO system, or predictive feedback algorithm. Unlike conventional adaptive optics methods which allow wavefront conjugation, the proposed total field conjugation technique is critical for beam control in the presence of strong turbulence and can be achieved by using this non-linear BEFWM technique. The phase information retrieved from the established beacon beam can then be used in conjunction with an AO system to propagate laser beams in deep turbulence.

Audible Beaconing with Accessible Pedestrian Signals

PubMed Central

Barlow, Janet M.; Scott, Alan C.; Bentzen, Billie Louise

2010-01-01

Purpose Although Accessible Pedestrian Signals (APS) are often assumed to provide wayfinding information, the type of APS that has been typically installed in the U.S has not had positive effects on finding crosswalks, locating pushbuttons, or providing directional guidance. This paper reports the results of research on crossings by blind pedestrians at complex signalized intersections, before and after the installation of APS with innovative audible beaconing features, designed to improve wayfinding. Methods Objective data on measures of street crossing performance by 56 participants was obtained at four intersections, two each in Charlotte, NC, and Portland, OR. Results In the first round of testing, APS with beaconing features resulted in only slightly improved wayfinding. Revisions to the audible beaconing features resulted in improved performance on four measures of wayfinding as compared to the pre-installation condition: beginning crossings within the crosswalk, ending crossings within the crosswalk, independence in finding the starting location, and independence in aligning to cross. Implications for Practice Use of APS that provide beaconing from the far-end of the crosswalk show promise of improving wayfinding at street crossings. PMID:20622978

Impacts of Fog Characteristics, Forward Illumination, and Warning Beacon Intensity Distribution on Roadway Hazard Visibility

PubMed Central

2016-01-01

Warning beacons are critical for the safety of transportation, construction, and utility workers. These devices need to produce sufficient luminous intensity to be visible without creating glare to drivers. Published standards for the photometric performance of warning beacons do not address their performance in conditions of reduced visibility such as fog. Under such conditions light emitted in directions other than toward approaching drivers can create scattered light that makes workers and other hazards less visible. Simulations of visibility of hazards under varying conditions of fog density, forward vehicle lighting, warning beacon luminous intensity, and intensity distribution were performed to assess their impacts on visual performance by drivers. Each of these factors can influence the ability of drivers to detect and identify workers and hazards along the roadway in work zones. Based on the results, it would be reasonable to specify maximum limits on the luminous intensity of warning beacons in directions that are unlikely to be seen by drivers along the roadway, limits which are not included in published performance specifications. PMID:27314058

16S rRNA beacons for bacterial monitoring during human space missions.

PubMed

Larios-Sanz, Maia; Kourentzi, Katerina D; Warmflash, David; Jones, Jeffrey; Pierson, Duane L; Willson, Richard C; Fox, George E

2007-04-01

Microorganisms are unavoidable in space environments and their presence has, at times, been a source of problems. Concerns about disease during human space missions are particularly important considering the significant changes the immune system incurs during spaceflight and the history of microbial contamination aboard the Mir space station. Additionally, these contaminants may have adverse effects on instrumentation and life-support systems. A sensitive, highly specific system to detect, characterize, and monitor these microbial populations is essential. Herein we describe a monitoring approach that uses 16S rRNA targeted molecular beacons to successfully detect several specific bacterial groupings. This methodology will greatly simplify in-flight monitoring by minimizing sample handling and processing. We also address and provide solutions to target accessibility problems encountered in hybridizations that target 16S rRNA.

Note: Methodology for the analysis of Bluetooth gateways in an implemented scatternet.

PubMed

Etxaniz, J; Monje, P M; Aranguren, G

2014-03-01

This Note introduces a novel methodology to analyze the time performance of Bluetooth gateways in multi-hop networks, known as scatternets. The methodology is focused on distinguishing between the processing time and the time that each communication between nodes takes along an implemented scatternet. This technique is not only valid for Bluetooth networks but also for other wireless networks that offer access to their middleware in order to include beacons in the operation of the nodes. We show in this Note the results of the tests carried out on a Bluetooth scatternet in order to highlight the reliability and effectiveness of the methodology. The results also validate this technique showing convergence in the results when subtracting the time for the beacons from the delay measurements.

Creating a Connected Community: Lessons Learned from the Western New York Beacon Community

PubMed Central

Maloney, Nancy; Heider, Arvela R.; Rockwood, Amy; Singh, Ranjit

2014-01-01

Introduction: Secure exchange of clinical data among providers has the potential to improve quality, safety, efficiency, and reduce duplication. Many communities are experiencing challenges in building effective health information exchanges (HIEs). Previous studies have focused on financial and technical issues regarding HIE development. This paper describes the Western New York (WNY) HIE growth and lessons learned about accelerating progress to become a highly connected community. Methods: HEALTHeLINK, with funding from the Office of the National Coordinator for Health Information Technology (ONC) under the Beacon Community Program, expanded HIE usage in eight counties. The communitywide transformation process used three main drivers: (1) a communitywide Electronic Health Record (EHR) adoption program; (2) clinical transformation partners; and (3) HIE outreach and infrastructure development. Results: ONC Beacon Community funding allowed WNY to achieve a new level in the use of interoperable HIE. Electronic delivery of results into the EHR expanded from 23 practices in 2010 to 222 practices in 2013, a tenfold increase. There were more than 12.5 million results delivered electronically (HL7 messages) to 222 practices’ EHRs via the HIE in 2013. Use of a secure portal and Virtual Health Record (VHR) to access reports (those not delivered directly to the EHR) also increased significantly, from 13,344 report views in 2010 to over 600,000 in 2013. Discussion and Conclusion: The WNY Beacon successfully expanded the sharing of clinical information among different sources of data and providers, creating a highly connected community to improve the quality and continuity of care. Technical, organizational, and community lessons described in this paper should prove beneficial to others as they pursue efforts to create connected communities. PMID:25848618

Gangliosidoses

MedlinePlus

... Diseases Association 2001 Beacon Street Suite 204 Boston MA Boston, MA 02135 info@ntsad.org http://www.ntsad.org ... Diseases Association 2001 Beacon Street Suite 204 Boston MA Boston, MA 02135 info@ntsad.org http://www. ...

14 CFR 171.205 - Minimum requirements for approval.

Code of Federal Regulations, 2010 CFR

2010-01-01

... (CONTINUED) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES VHF Marker Beacons § 171.205 Minimum... marker beacon facility under this subpart: (1) The facility's performances, as determined by air and...

14 CFR 171.23 - Requests for IFR procedure.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Nondirectional Radio Beacon Facilities § 171.23... beacon facility that he owns must submit the following information with that request: (1) A description...

Aspect Determination Using a Beacon with a Spiral Wave Front: Modeling and Performance Analysis in Operational Environments

DTIC Science & Technology

2014-12-19

used to evaluate the beacon performance at the Navy’s Seneca Lake Sonar Test Facility operated by NUWC-Newport. These tests occurred in the summer...prototype has been designed. Efforts have been underway to implement the spiral beacon into the Navy’s Sonar Simulation Toolset developed by Dr. Robert...mil). Digital Object Identifier 10.1109/JOE.2013.2293962 acoustic depth finding or sonar imaging may be compared with maps to coordinate position and

14 CFR 171.201 - Scope.

Code of Federal Regulations, 2010 CFR

2010-01-01

... FACILITIES NON-FEDERAL NAVIGATION FACILITIES VHF Marker Beacons § 171.201 Scope. (a) This subpart sets forth minimum requirements for the approval and operation of non-Federal VHF marker beacon facilities that are...

14 CFR 171.203 - Requests for IFR procedure.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES VHF Marker Beacons § 171.203 Requests for IFR... beacon facility that he owns must submit the following information with that request: (1) A description...

Design and Analysis of A Beacon-Less Routing Protocol for Large Volume Content Dissemination in Vehicular Ad Hoc Networks.

PubMed

Hu, Miao; Zhong, Zhangdui; Ni, Minming; Baiocchi, Andrea

2016-11-01

Large volume content dissemination is pursued by the growing number of high quality applications for Vehicular Ad hoc NETworks(VANETs), e.g., the live road surveillance service and the video-based overtaking assistant service. For the highly dynamical vehicular network topology, beacon-less routing protocols have been proven to be efficient in achieving a balance between the system performance and the control overhead. However, to the authors' best knowledge, the routing design for large volume content has not been well considered in the previous work, which will introduce new challenges, e.g., the enhanced connectivity requirement for a radio link. In this paper, a link Lifetime-aware Beacon-less Routing Protocol (LBRP) is designed for large volume content delivery in VANETs. Each vehicle makes the forwarding decision based on the message header information and its current state, including the speed and position information. A semi-Markov process analytical model is proposed to evaluate the expected delay in constructing one routing path for LBRP. Simulations show that the proposed LBRP scheme outperforms the traditional dissemination protocols in providing a low end-to-end delay. The analytical model is shown to exhibit a good match on the delay estimation with Monte Carlo simulations, as well.

A Two-Layer Method for Sedentary Behaviors Classification Using Smartphone and Bluetooth Beacons.

PubMed

Cerón, Jesús D; López, Diego M; Hofmann, Christian

2017-01-01

Among the factors that outline the health of populations, person's lifestyle is the more important one. This work focuses on the caracterization and prevention of sedentary lifestyles. A sedentary behavior is defined as "any waking behavior characterized by an energy expenditure of 1.5 METs (Metabolic Equivalent) or less while in a sitting or reclining posture". To propose a method for sedentary behaviors classification using a smartphone and Bluetooth beacons considering different types of classification models: personal, hybrid or impersonal. Following the CRISP-DM methodology, a method based on a two-layer approach for the classification of sedentary behaviors is proposed. Using data collected from a smartphones' accelerometer, gyroscope and barometer; the first layer classifies between performing a sedentary behavior and not. The second layer of the method classifies the specific sedentary activity performed using only the smartphone's accelerometer and barometer data, but adding indoor location data, using Bluetooth Low Energy (BLE) beacons. To improve the precision of the classification, both layers implemented the Random Forest algorithm and the personal model. This study presents the first available method for the automatic classification of specific sedentary behaviors. The layered classification approach has the potential to improve processing, memory and energy consumption of mobile devices and wearables used.

Multiplex detection of microRNAs by combining molecular beacon probes with T7 exonuclease-assisted cyclic amplification reaction.

PubMed

Liu, Yacui; Zhang, Jiangyan; Tian, Jingxiao; Fan, Xiaofei; Geng, Hao; Cheng, Yongqiang

2017-01-01

A simple, highly sensitive, and specific assay was developed for the homogeneous and multiplex detection of microRNAs (miRNAs) by combining molecular beacon (MB) probes and T7 exonuclease-assisted cyclic amplification. An MB probe with five base pairs in the stem region without special modification can effectively prevent the digestion by T7 exonuclease. Only in the presence of target miRNA is the MB probe hybridized with the target miRNA, and then digested by T7 exonuclease in the 5' to 3' direction. At the same time, the target miRNA is released and subsequently initiates the nuclease-assisted cyclic digestion process, generating enhanced fluorescence signal significantly. The results show that the combination of T7 exonuclease-assisted cyclic amplification reaction and MB probe possesses higher sensitivity for miRNA detection. Moreover, multiplex detection of miRNAs was successfully achieved by designing two MB probes labeled with FAM and Cy3, respectively. As a result, the method opens a new pathway for the sensitive and multiplex detection of miRNAs as well as clinical diagnosis. Graphical Abstract A simple, highly sensitive, and specific assay was developed for the detection of microRNAs by combining molecular beacon probes with T7 exonuclease-assisted cyclic amplification reaction.

Design and Analysis of A Beacon-Less Routing Protocol for Large Volume Content Dissemination in Vehicular Ad Hoc Networks

PubMed Central

Hu, Miao; Zhong, Zhangdui; Ni, Minming; Baiocchi, Andrea

2016-01-01

Large volume content dissemination is pursued by the growing number of high quality applications for Vehicular Ad hoc NETworks(VANETs), e.g., the live road surveillance service and the video-based overtaking assistant service. For the highly dynamical vehicular network topology, beacon-less routing protocols have been proven to be efficient in achieving a balance between the system performance and the control overhead. However, to the authors’ best knowledge, the routing design for large volume content has not been well considered in the previous work, which will introduce new challenges, e.g., the enhanced connectivity requirement for a radio link. In this paper, a link Lifetime-aware Beacon-less Routing Protocol (LBRP) is designed for large volume content delivery in VANETs. Each vehicle makes the forwarding decision based on the message header information and its current state, including the speed and position information. A semi-Markov process analytical model is proposed to evaluate the expected delay in constructing one routing path for LBRP. Simulations show that the proposed LBRP scheme outperforms the traditional dissemination protocols in providing a low end-to-end delay. The analytical model is shown to exhibit a good match on the delay estimation with Monte Carlo simulations, as well. PMID:27809285

Visual format for detection of Mycobacterium tuberculosis and M. bovis in clinical samples using molecular beacons.

PubMed

Kumar, Parameet; Nath, Kapili; Rath, Bimba; Sen, Manas K; Vishalakshi, Potharuju; Chauhan, Devender S; Katoch, Vishwa M; Singh, Sarman; Tyagi, Sanjay; Sreenivas, Vishnubhatla; Prasad, Hanumanthappa K

2009-09-01

A real-time polymerase chain reaction (PCR) assay for the direct identification of Mycobacterium tuberculosis and M. bovis using molecular beacons was developed. The assay was modified for use in regular thermal cyclers. Molecular beacons that were specific for M. tuberculosis (Tb-B) and M. bovis (Bo-B) were designed. The fluorescence of the target PCR product-molecular beacon probe complex was detected visually using a transilluminator. The results were then compared with those of conventional multiplex PCR (CM-PCR) assays and biochemical identification. The detection limit of Tb-B and Bo-B beacons was 500 fg and 50 fg by the visual format and real-time PCR assay, respectively, compared with 5 pg by CM-PCR assay. Pulmonary and extrapulmonary samples were examined. The agreement between culture and the two assays was very good in sputum samples and fair in extrapulmonary samples. The agreement between clinical diagnoses with the two assays was moderate in extrapulmonary samples. There was very good agreement between CM-PCR and visual format assays for all samples used in the study. Concordance in the identification of isolates by the visual, CM-PCR assay, and biochemical identification was seen. Hence, the use of molecular beacon detection of M. tuberculosis and M. bovis in clinical samples is feasible by setting up two asymmetric PCRs concurrently. The assay is sensitive, specific, simple to interpret, and takes less than 3 hours to complete.

Visual Format for Detection of Mycobacterium tuberculosis and M. bovis in Clinical Samples Using Molecular Beacons

PubMed Central

Kumar, Parameet; Nath, Kapili; Rath, Bimba; Sen, Manas K.; Vishalakshi, Potharuju; Chauhan, Devender S.; Katoch, Vishwa M.; Singh, Sarman; Tyagi, Sanjay; Sreenivas, Vishnubhatla; Prasad, Hanumanthappa K.

2009-01-01

A real-time polymerase chain reaction (PCR) assay for the direct identification of Mycobacterium tuberculosis and M. bovis using molecular beacons was developed. The assay was modified for use in regular thermal cyclers. Molecular beacons that were specific for M. tuberculosis (Tb-B) and M. bovis (Bo-B) were designed. The fluorescence of the target PCR product-molecular beacon probe complex was detected visually using a transilluminator. The results were then compared with those of conventional multiplex PCR (CM-PCR) assays and biochemical identification. The detection limit of Tb-B and Bo-B beacons was 500 fg and 50 fg by the visual format and real-time PCR assay, respectively, compared with 5 pg by CM-PCR assay. Pulmonary and extrapulmonary samples were examined. The agreement between culture and the two assays was very good in sputum samples and fair in extrapulmonary samples. The agreement between clinical diagnoses with the two assays was moderate in extrapulmonary samples. There was very good agreement between CM-PCR and visual format assays for all samples used in the study. Concordance in the identification of isolates by the visual, CM-PCR assay, and biochemical identification was seen. Hence, the use of molecular beacon detection of M. tuberculosis and M. bovis in clinical samples is feasible by setting up two asymmetric PCRs concurrently. The assay is sensitive, specific, simple to interpret, and takes less than 3 hours to complete. PMID:19661384

Ultrasonically-Induced Vaporization of Perfluorocarbon Droplets for Occlusion Therapy of Breast Cancer

DTIC Science & Technology

2004-06-01

lithotripsy applications. Their focusing process can be iterated to work on several point beacons. Other methods compute the angular spectrum, i.e., the...99 [10] Hutton, S., P. 1972 Inaugural Lecture University of Southampton [11] Trevena, D. H. (1984). “Cavitation and the Generation of Tension in

Improved fixation quality provided by a Bessel beacon in an adaptive optics system.

PubMed

Lambert, Andrew J; Daly, Elizabeth M; Dainty, Christopher J

2013-07-01

We investigate whether a structured probe beam that creates the beacon for use in a retinal imaging adaptive optics system can provide useful side effects. In particular we investigate whether a Bessel beam that is seen by the subject as a set of concentric rings has a dampening effect on fixation variations of the subject under observation. This calming effect would allow longer periods of observation, particularly for patients with abnormal fixation. An experimental adaptive optics system developed for retinal imaging is used to monitor the fluctuations in aberrations for artificial and human subjects. The probe beam is alternated between a traditional beacon and one provided by a Bessel beam created by SLM. Time-frequency analysis is used to indicate the differences in power and time variation during fixation depending on whether the Bessel beam or the traditional beacon is employed. Comparison is made with the response for an artificial eye to discount systemic variations. Significant evidence is accrued to indicate the reduced fluctuations in fixation when the Bessel beam is employed to create the beacon. © 2013 The Authors Ophthalmic & Physiological Optics © 2013 The College of Optometrists.

Reputation-Based Secure Sensor Localization in Wireless Sensor Networks

PubMed Central

He, Jingsha; Xu, Jing; Zhu, Xingye; Zhang, Yuqiang; Zhang, Ting; Fu, Wanqing

2014-01-01

Location information of sensor nodes in wireless sensor networks (WSNs) is very important, for it makes information that is collected and reported by the sensor nodes spatially meaningful for applications. Since most current sensor localization schemes rely on location information that is provided by beacon nodes for the regular sensor nodes to locate themselves, the accuracy of localization depends on the accuracy of location information from the beacon nodes. Therefore, the security and reliability of the beacon nodes become critical in the localization of regular sensor nodes. In this paper, we propose a reputation-based security scheme for sensor localization to improve the security and the accuracy of sensor localization in hostile or untrusted environments. In our proposed scheme, the reputation of each beacon node is evaluated based on a reputation evaluation model so that regular sensor nodes can get credible location information from highly reputable beacon nodes to accomplish localization. We also perform a set of simulation experiments to demonstrate the effectiveness of the proposed reputation-based security scheme. And our simulation results show that the proposed security scheme can enhance the security and, hence, improve the accuracy of sensor localization in hostile or untrusted environments. PMID:24982940

Road User Behaviors At Pedestrian Hybrid Beacons

DOT National Transportation Integrated Search

2016-06-01

The pedestrian hybrid beacon (PHB) has shown great potential for improving pedestrian safety and driver yielding. However, questions remain regarding under what roadway conditions such as crossing distance (i.e., number of lanes) and posted speed ...

Development and flight test of a helicopter compact, portable, precision landing system concept

NASA Technical Reports Server (NTRS)

Clary, G. R.; Bull, J. S.; Davis, T. J.; Chisholm, J. P.

1984-01-01

An airborne, radar-based, precision approach concept is being developed and flight tested as a part of NASA's Rotorcraft All-Weather Operations Research Program. A transponder-based beacon landing system (BLS) applying state-of-the-art X-band radar technology and digital processing techniques, was built and is being flight tested to demonstrate the concept feasibility. The BLS airborne hardware consists of an add-on microprocessor, installed in conjunction with the aircraft weather/mapping radar, which analyzes the radar beacon receiver returns and determines range, localizer deviation, and glide-slope deviation. The ground station is an inexpensive, portable unit which can be quickly deployed at a landing site. Results from the flight test program show that the BLS concept has a significant potential for providing rotorcraft with low-cost, precision instrument approach capability in remote areas.

A Tree Based Self-routing Scheme for Mobility Support in Wireless Sensor Networks

NASA Astrophysics Data System (ADS)

Kim, Young-Duk; Yang, Yeon-Mo; Kang, Won-Seok; Kim, Jin-Wook; An, Jinung

Recently, WSNs (Wireless Sensor Networks) with mobile robot is a growing technology that offer efficient communication services for anytime and anywhere applications. However, the tiny sensor node has very limited network resources due to its low battery power, low data rate, node mobility, and channel interference constraint between neighbors. Thus, in this paper, we proposed a tree based self-routing protocol for autonomous mobile robots based on beacon mode and implemented in real test-bed environments. The proposed scheme offers beacon based real-time scheduling for reliable association process between parent and child nodes. In addition, it supports smooth handover procedure by reducing flooding overhead of control packets. Throughout the performance evaluation by using a real test-bed system and simulation, we illustrate that our proposed scheme demonstrates promising performance for wireless sensor networks with mobile robots.

Frequency Estimator Performance for a Software-Based Beacon Receiver

NASA Technical Reports Server (NTRS)

Zemba, Michael J.; Morse, Jacquelynne Rose; Nessel, James A.; Miranda, Felix

2014-01-01

As propagation terminals have evolved, their design has trended more toward a software-based approach that facilitates convenient adjustment and customization of the receiver algorithms. One potential improvement is the implementation of a frequency estimation algorithm, through which the primary frequency component of the received signal can be estimated with a much greater resolution than with a simple peak search of the FFT spectrum. To select an estimator for usage in a QV-band beacon receiver, analysis of six frequency estimators was conducted to characterize their effectiveness as they relate to beacon receiver design.

Time-Resolved Nucleic Acid Hybridization Beacons Utilizing Unimolecular and Toehold-Mediated Strand Displacement Designs.

PubMed

Massey, Melissa; Ancona, Mario G; Medintz, Igor L; Algar, W Russ

2015-12-01

Nucleic acid hybridization probes are sought after for numerous assay and imaging applications. These probes are often limited by the properties of fluorescent dyes, prompting the development of new probes where dyes are paired with novel or nontraditional luminescent materials. Luminescent terbium complexes are an example of such a material, and these complexes offer several unique spectroscopic advantages. Here, we demonstrate two nonstem-loop designs for light-up nucleic acid hybridization beacons that utilize time-resolved Förster resonance energy transfer (TR-FRET) between a luminescent Lumi4-Tb cryptate (Tb) donor and a fluorescent reporter dye, where time-resolved emission from the dye provides an analytical signal. Both designs are based on probe oligonucleotides that are labeled at their opposite termini with Tb and a fluorescent reporter dye. In one design, a probe is partially blocked with a quencher dye-labeled oligonucleotide, and target hybridization is signaled through toehold-mediated strand displacement and loss of a competitive FRET pathway. In the other design, the intrinsic folding properties of an unblocked probe are utilized in combination with a temporal mechanism for signaling target hybridization. This temporal mechanism is based on a recently elucidated "sweet spot" for TR-FRET measurements and exploits distance control over FRET efficiencies to shift the Tb lifetime within or outside the time-gated detection window for measurements. Both the blocked and unblocked beacons offer nanomolar (femtomole) detection limits, response times on the order of minutes, multiplexing through the use of different reporter dyes, and detection in complex matrices such as serum and blood. The blocked beacons offer better mismatch selectivity, whereas the unblocked beacons are simpler in design. The temporal mechanism of signaling utilized with the unblocked beacons also plays a significant role with the blocked beacons and represents a new and effective strategy for developing FRET probes for bioassays.

Beacon Communities’ Public Health Initiatives: A Case Study Analysis

PubMed Central

Massoudi, Barbara L.; Marcial, Laura H.; Haque, Saira; Bailey, Robert; Chester, Kelley; Cunningham, Shellery; Riley, Amanda; Soper, Paula

2014-01-01

Introduction: The Beacon Communities for Public Health (BCPH) project was launched in 2011 to gain a better understanding of the range of activities currently being conducted in population- and public health by the Beacon Communities. The project highlighted the successes and challenges of these efforts with the aim of sharing this information broadly among the public health community. Background: The Beacon Community Program, designed to showcase technology-enabled, community-based initiatives to improve outcomes, focused on: building and strengthening health information technology (IT) infrastructure and exchange capabilities; translating investments in health IT to measureable improvements in cost, quality, and population health; and, developing innovative approaches to performance measurement, technology, and care delivery. Methods: Four multimethod case studies were conducted based on a modified sociotechnical framework to learn more about public health initiative implementation and use in the Beacon Communities. Our methodological approach included using document review and semistructured key informant interviews. NACCHO Model Practice Program criteria were used to select the public health initiatives included in the case studies. Findings: Despite differences among the case studies, common barriers and facilitators were found to be present in all areas of the sociotechnical framework application including structure, people, technology, tasks, overarching considerations, and sustainability. Overall, there were many more facilitators (range = 7–14) present for each Beacon compared to barriers (range = 4–6). Discussion: Four influential promising practices were identified through the work: forging strong and sustainable partnerships; ensuring a good task-technology fit and a flexible and iterative design; fostering technology acceptance; and, providing education and demonstrating value. Conclusions: A common weakness was the lack of a framework or model for the Beacon Communities evaluation work. Sharing a framework or approach to evaluation at the beginning of implementation made the work more effective. Supporting evaluation to inform future implementations is important. PMID:25848620

47 CFR 95.1402 - Special requirements for 406 MHz PLBs.

Code of Federal Regulations, 2010 CFR

2010-10-01

...: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200 Auth Road, Suitland, Maryland... Administration (NOAA) whose address is: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200...

47 CFR 95.1402 - Special requirements for 406 MHz PLBs.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200 Auth Road, Suitland, Maryland... Administration (NOAA) whose address is: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200...

47 CFR 95.1402 - Special requirements for 406 MHz PLBs.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200 Auth Road, Suitland, Maryland... Administration (NOAA) whose address is: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200...

47 CFR 95.1402 - Special requirements for 406 MHz PLBs.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200 Auth Road, Suitland, Maryland... Administration (NOAA) whose address is: SARSAT Beacon Registration, NOAA, NESDIS, E/SP3, Room 3320, FB-4, 5200...

Evaluation of light-emitting diode beacon light fixtures.

DOT National Transportation Integrated Search

2009-12-01

Rotating beacons containing filament light sources have long been used on highway maintenance trucks : to indicate the presence of the truck to other drivers. Because of advances in light-emitting diode (LED) : technologies, flashing lights containin...

Hybrid diagnostic system: beacon-based exception analysis for multimissions - Livingstone integration

NASA Technical Reports Server (NTRS)

Park, Han G.; Cannon, Howard; Bajwa, Anupa; Mackey, Ryan; James, Mark; Maul, William

2004-01-01

This paper describes the initial integration of a hybrid reasoning system utilizing a continuous domain feature-based detector, Beacon-based Exceptions Analysis for Multimissions (BEAM), and a discrete domain model-based reasoner, Livingstone.

78 FR 21043 - Amendment of Class D and Class E Airspace; Caldwell, NJ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-09

... Class E Airspace at Caldwell, NJ as the Paterson Non-Directional Radio Beacon (NDB) has been..., Caldwell, NJ. The Patterson Non-Directional Beacon has been decommissioned, and the NDB approach cancelled...

14 CFR 91.217 - Data correspondence between automatically reported pressure altitude data and the pilot's...

Code of Federal Regulations, 2011 CFR

2011-01-01

... radar beacon transponder— (1) When deactivation of that equipment is directed by ATC; (2) Unless, as... operate any automatic pressure altitude reporting equipment associated with a radar beacon transponder or...

Evaluation of alternative pedestrian control devices.

DOT National Transportation Integrated Search

2012-03-01

A literature review, field study of Rectangular Rapid Flashing Beacon (RRFB) installations in Oregon, and a static survey : on the sequencing of the Pedestrian Hybrid Beacon (PHB) were completed. : The field study conducted in this project was design...

Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing.

PubMed

Guo, Bingyuan; Sheng, Yingying; Zhou, Ke; Liu, Quansheng; Liu, Lei; Wu, Hai-Chen

2018-03-26

A new nanopore sensing strategy based on triplex molecular beacon was developed for the detection of specific DNA or multivalent proteins. The sensor is composed of a triplex-forming molecular beacon and a stem-forming DNA component that is modified with a host-guest complex. Upon target DNA hybridizing with the molecular beacon loop or multivalent proteins binding to the recognition elements on the stem, the DNA probe is released and produces highly characteristic current signals when translocated through α-hemolysin. The frequency of current signatures can be used to quantify the concentrations of the target molecules. This sensing approach provides a simple, quick, and modular tool for the detection of specific macromolecules with high sensitivity and excellent selectivity. It may find useful applications in point-of-care diagnostics with a portable nanopore kit in the future. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus.

PubMed

Mandappa, I M; Joglekar, Prasanna; Manonmani, H K

2015-07-01

A multiplex real-time isothermal amplification assay was developed using molecular beacons for the detection of Bacillus cereus and Staphylococcus aureus by targeting four important virulence genes. A correlation between targeting highly accessible DNA sequences and isothermal amplification based molecular beacon efficiency and sensitivity was demonstrated using phi(Φ)29 DNA polymerase at a constant isothermal temperature of 30 °C. It was very selective and consistently detected down to 10(1) copies of DNA. The specificity and sensitivity of this assay, when tested with pure culture were high, surpassing those of currently used PCR assays for the detection of these organisms. The molecular beacon based real-time isothermal amplification (MBRTIA) assay could be carried out entirely in 96 well plates or well strips, enabling a rapid and high-throughput detection of food borne pathogens.

A beacon interval shifting scheme for interference mitigation in body area networks.

PubMed

Kim, Seungku; Kim, Seokhwan; Kim, Jin-Woo; Eom, Doo-Seop

2012-01-01

This paper investigates the issue of interference avoidance in body area networks (BANs). IEEE 802.15 Task Group 6 presented several schemes to reduce such interference, but these schemes are still not proper solutions for BANs. We present a novel distributed TDMA-based beacon interval shifting scheme that reduces interference in the BANs. A design goal of the scheme is to avoid the wakeup period of each BAN coinciding with other networks by employing carrier sensing before a beacon transmission. We analyze the beacon interval shifting scheme and investigate the proper back-off length when the channel is busy. We compare the performance of the proposed scheme with the schemes presented in IEEE 802.15 Task Group 6 using an OMNeT++ simulation. The simulation results show that the proposed scheme has a lower packet loss, energy consumption, and delivery-latency than the schemes of IEEE 802.15 Task Group 6.

Error Estimation for the Linearized Auto-Localization Algorithm

PubMed Central

Guevara, Jorge; Jiménez, Antonio R.; Prieto, Jose Carlos; Seco, Fernando

2012-01-01

The Linearized Auto-Localization (LAL) algorithm estimates the position of beacon nodes in Local Positioning Systems (LPSs), using only the distance measurements to a mobile node whose position is also unknown. The LAL algorithm calculates the inter-beacon distances, used for the estimation of the beacons’ positions, from the linearized trilateration equations. In this paper we propose a method to estimate the propagation of the errors of the inter-beacon distances obtained with the LAL algorithm, based on a first order Taylor approximation of the equations. Since the method depends on such approximation, a confidence parameter τ is defined to measure the reliability of the estimated error. Field evaluations showed that by applying this information to an improved weighted-based auto-localization algorithm (WLAL), the standard deviation of the inter-beacon distances can be improved by more than 30% on average with respect to the original LAL method. PMID:22736965

A Beacon Interval Shifting Scheme for Interference Mitigation in Body Area Networks

PubMed Central

Kim, Seungku; Kim, Seokhwan; Kim, Jin-Woo; Eom, Doo-Seop

2012-01-01

This paper investigates the issue of interference avoidance in body area networks (BANs). IEEE 802.15 Task Group 6 presented several schemes to reduce such interference, but these schemes are still not proper solutions for BANs. We present a novel distributed TDMA-based beacon interval shifting scheme that reduces interference in the BANs. A design goal of the scheme is to avoid the wakeup period of each BAN coinciding with other networks by employing carrier sensing before a beacon transmission. We analyze the beacon interval shifting scheme and investigate the proper back-off length when the channel is busy. We compare the performance of the proposed scheme with the schemes presented in IEEE 802.15 Task Group 6 using an OMNeT++ simulation. The simulation results show that the proposed scheme has a lower packet loss, energy consumption, and delivery-latency than the schemes of IEEE 802.15 Task Group 6. PMID:23112639

Beacon of Hope? Lessons Learned from Efforts to Reduce Civilian Deaths from Police Shootings in an Australian State.

PubMed

Saligari, Jessica; Evans, Richard

2016-04-01

In the 1990s, the police service in Victoria, Australia, faced a crisis of community confidence due to a spate of civilian deaths from police shootings. In that decade, twice as many civilians died at the hands of the police in Victoria than in every other Australian state combined. Most of those killed were mentally ill and affected by drugs and alcohol, and were rarely a serious threat except to themselves. The problem was also almost entirely an urban phenomenon. Shootings in rural communities, where mentally ill people were more likely to be personally known to local police, were (and remain) almost unknown. The large number of fatalities was recognised as a serious threat to public confidence, and Victoria Police introduced a ground-breaking training programme, Operation Beacon. Operating procedures and weapons training were fundamentally changed, to focus on de-escalation of conflict and avoiding or minimising police use of force. In the short term, Operation Beacon was successful. Shooting incidents were dramatically reduced. However, during the first decade of the new century, the number of civilians being killed again increased. This article examines Operation Beacon, both as a successful model for reducing civilian deaths at the hand of police and as a cautionary tale for police reform. We argue that the lessons of Operation Beacon have been gradually forgotten and that old habits and attitudes resurfaced. Fatal shootings of mentally ill civilians can be prevented, but if success is to be other than temporary, the Beacon philosophy must be continually reemphasised by police management.

Double-hairpin molecular-beacon-based amplification detection for gene diagnosis linked to cancer.

PubMed

Xu, Huo; Zhang, Rongbo; Li, Feng; Zhou, Yingying; Peng, Ting; Wang, Xuedong; Shen, Zhifa

2016-09-01

A powerful double-hairpin molecular beacon (DHMB) was developed for cancer-related KRAS gene detection based on the one-to-two stoichiometry. During target DNA detection, DHMB can execute signal transduction even if no any exogenous element is involved. Unlike the conventional molecular beacon based on the one-to-one interaction, one target DNA not only hybridizes with one DHMB and opens its hairpin but also promotes the interaction between two DHMBs, causing the separation of two fluorophores from quenchers. This leads to an enhanced fluorescence signal. As a result, the target KRAS gene is able to be detected within a wide dynamic range from 0.05 to 200 nM with the detection limit of 50 pM, indicating a dramatic improvement compared with traditional molecular beacons. Moreover, the point mutations existing in target DNAs can be easily screened. The potential application for target species in real samples was indicated by the analysis of PCR amplicons of DNAs from the DNA extracted from SW620 cell. Besides becoming a promising candidate probe for molecular biology research and clinical diagnosis of genetic diseases, the DHMB is expected to provide a significant insight into the design of DNA probe-based homogenous sensing systems. Graphical Abstract A powerful double-hairpin molecular beacon (DHMB) was developed for cancer-related gene KRAS detection based on the one-to-two stoichiometry. Without the help of any exogenous probe, the point mutation is easily screened, and the target DNA can be quantified down to 50 pM, indicating a dramatic improvement compared with traditional molecular beacons.

Evaluation of light-emitting diode beacon light fixtures : final report.

DOT National Transportation Integrated Search

2009-12-01

Rotating beacons containing filament light sources have long been used on highway maintenance trucks : to indicate the presence of the truck to other drivers. Because of advances in light-emitting diode (LED) : technologies, flashing lights containin...

GaAs monolithic R.F. modules for SARSAT distress beacons

NASA Technical Reports Server (NTRS)

Cauley, Michael A.

1991-01-01

Monolithic GaAs UHF components for use in SARSAT Emergency Distress beacons are under development by Microwave Monolithics, Inc., Simi Valley, CA. The components include a bi-phase modulator, driver amplifier, and a 5 watt power amplifier.

GaAs monolithic RF modules for SARSAT distress beacons

NASA Technical Reports Server (NTRS)

Cauley, Michael A.

1991-01-01

Monolithic GaAs UHF components for use in SARSAT Emergency Distress beacons are under development by Microwave Monolithics, Inc., Simi Valley, CA. The components include a bi-phase modulator, driver amplifier, and a 5 watt power amplifier.

Pedestrian hybrid beacon guide : recommendations and case study.

DOT National Transportation Integrated Search

2014-01-01

A pedestrian hybrid beacon (PHB) is a traffic control : device similar to a European pedestrian signal : (PELICAN) that was imported to the US and adapted by : engineers in Arizona to increase motorists awareness of : pedestrian crossings at uncon...

Beacon Collision Avoidance System (BCAS) Alternative Concepts for Determining Target Positions

DOT National Transportation Integrated Search

1978-09-01

The (Litchford) Beacon-based Collision Avoidance System concept requires the computation of target range and bearing relative to the BCAS aircraft. Techniques for determining target range and bearing under four different assumptions about the ground ...

VHF-FM Emergency Position Indicating Radio Beacon

DOT National Transportation Integrated Search

1978-10-01

This report describes the development and testing of an Emergency Position Indicating Radio Beacon (EPIRB) which operates on Channels 15 and 16 of the Maritime Mobile VHF Band. It provides functions necessary to ensure that distress alerting and loca...

A MURI Center for Intelligent Biomimetic Image Processing and Classification

DTIC Science & Technology

2007-11-01

Grossberg, S., Editor of a new journal on Current Opinion in Cognitive Neurodynamics , 2005. 34. Grossberg, S., Editor of the new International...Department of Cognitive and Neural Systems 677 Beacon Street Boston MA 02215 steve(&bu.edu Phone: 617-353-7857 Fax: 617-353-7755 http://cns.bu.edu...memory, learning of sequential plans, and sequence performance during cognitive information processing; (8) coordinated ballistic and smooth pursuit

75 FR 11216 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change Relating...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-10

..., 2009) (SR-NYSEArca-2009-83) (order approving listing of Grail American Beacon International Equity ETF... appreciation above international benchmarks, such as the BNY Mellon Classic ADR Index and the MSCI EAFE Index... process include demographics, global commerce, outsourcing, the growing global middle class and the...

Modulation and coding for a compatible Discrete Address Beacon System.

DOT National Transportation Integrated Search

1972-02-01

One of several possible candidate configurations for the Discrete Address System is described. The configuration presented is compatible with the Air Traffic Control Radar Beacon System, and it provides for gradual transition from one system to the o...

Beacons of X-ray Light Animation

NASA Image and Video Library

2014-10-08

This image shows a neutron star -- the core of a star that exploded in a massive supernova. This particular neutron star is known as a pulsar because it sends out rotating beams of X-rays that sweep past Earth like lighthouse beacons.

Beacon communities aim to use health information technology to transform the delivery of care.

PubMed

Maxson, Emily R; Jain, Sachin H; McKethan, Aaron N; Brammer, Craig; Buntin, Melinda Beeuwkes; Cronin, Kelly; Mostashari, Farzad; Blumenthal, David

2010-09-01

The Beacon Community Program, authorized under the 2009 American Recovery and Reinvestment Act (ARRA), aims to demonstrate the potential for health information technology to enable local improvements in health care quality, cost efficiency, and population health. If successful, these communitywide efforts will yield important lessons that will assist other communities seeking to harness technology to achieve and sustain health care improvements. This paper highlights key programmatic details that reflect the meaningful use of technology in the fifteen Beacon communities. It describes the innovations they propose and provides insight into current and future challenges.

Frequency Estimator Performance for a Software-Based Beacon Receiver

NASA Technical Reports Server (NTRS)

Zemba, Michael J.; Morse, Jacquelynne R.; Nessel, James A.

2014-01-01

As propagation terminals have evolved, their design has trended more toward a software-based approach that facilitates convenient adjustment and customization of the receiver algorithms. One potential improvement is the implementation of a frequency estimation algorithm, through which the primary frequency component of the received signal can be estimated with a much greater resolution than with a simple peak search of the FFT spectrum. To select an estimator for usage in a Q/V-band beacon receiver, analysis of six frequency estimators was conducted to characterize their effectiveness as they relate to beacon receiver design.

Sequential strand displacement beacon for detection of DNA coverage on functionalized gold nanoparticles.

PubMed

Paliwoda, Rebecca E; Li, Feng; Reid, Michael S; Lin, Yanwen; Le, X Chris

2014-06-17

Functionalizing nanomaterials for diverse analytical, biomedical, and therapeutic applications requires determination of surface coverage (or density) of DNA on nanomaterials. We describe a sequential strand displacement beacon assay that is able to quantify specific DNA sequences conjugated or coconjugated onto gold nanoparticles (AuNPs). Unlike the conventional fluorescence assay that requires the target DNA to be fluorescently labeled, the sequential strand displacement beacon method is able to quantify multiple unlabeled DNA oligonucleotides using a single (universal) strand displacement beacon. This unique feature is achieved by introducing two short unlabeled DNA probes for each specific DNA sequence and by performing sequential DNA strand displacement reactions. Varying the relative amounts of the specific DNA sequences and spacing DNA sequences during their coconjugation onto AuNPs results in different densities of the specific DNA on AuNP, ranging from 90 to 230 DNA molecules per AuNP. Results obtained from our sequential strand displacement beacon assay are consistent with those obtained from the conventional fluorescence assays. However, labeling of DNA with some fluorescent dyes, e.g., tetramethylrhodamine, alters DNA density on AuNP. The strand displacement strategy overcomes this problem by obviating direct labeling of the target DNA. This method has broad potential to facilitate more efficient design and characterization of novel multifunctional materials for diverse applications.

Method of identifying hairpin DNA probes by partial fold analysis

DOEpatents

Miller, Benjamin L [Penfield, NY; Strohsahl, Christopher M [Saugerties, NY

2009-10-06

Method of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.

Method of identifying hairpin DNA probes by partial fold analysis

DOEpatents

Miller, Benjamin L.; Strohsahl, Christopher M.

2008-10-28

Methods of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.

SPHERES: Design of a Formation Flying Testbed for ISS

NASA Astrophysics Data System (ADS)

Sell, S. W.; Chen, S. E.

2002-01-01

The SPHERES (Synchronized Position Hold Engage and Reorient Experimental Satellites) payload is an innovative formation-flying spacecraft testbed currently being developed for use internally aboard the International Space Station (ISS). The purpose of the testbed is to provide a cost-effective, long duration, replenishable, and easily reconfigurable platform with representative dynamics for the development and validation of metrology, formation flying, and autonomy algorithms. The testbed components consist of three 8-inch diameter free-flying "satellites," five ultrasound beacons, and an ISS laptop workstation. Each satellite is self-contained with on-board battery power, cold-gas propulsion (CO2), and processing systems. Satellites use two packs of eight standard AA batteries for approximately 90 minutes of lifetime while beacons last the duration of the mission powered by a single AA battery. The propulsion system uses pressurized carbon dioxide gas, stored in replaceable tanks, distributed through an adjustable regulator and associated tubing to twelve thrusters located on the faces of the satellites. A Texas Instruments C6701 DSP handles control algorithm data while an FPGA manages all sensor data, timing, and communication processes on the satellite. All three satellites communicate with each other and with the controlling laptop via a wireless RF link. Five ultrasound beacons, located around a predetermined work area, transmit ultrasound signals that are received by each satellite. The system effectively acts as a pseudo-GPS system, allowing the satellites to determine position and attitude and to navigate within the test arena. The payload hardware are predominantly Commercial Off The Shelf (COTS) products with the exception of custom electronics boards, selected propulsion system adaptors, and beacon and satellite structural elements. Operationally, SPHERES will run in short duration test sessions with approximately two weeks between each session. During operations, satellites will autonomously perform various maneuvers with one to three satellites operating simultaneously, involving a crew member only to upload protocols and replace satellite consumables (gas and power) during the test session. Once completed, data will be downlinked to the ground for analysis by the SPHERES team, facilitating the iterative process of new and/or modified protocols being uplinked for use in the next test session. SPHERES has prior flight experience on the NASA KC-135 Reduced Gravity aircraft and has also been in constant use in laboratory air table testing for almost two years. Slated for launch to the International Space Station on ISS12A.1, SPHERES will use its six-month flight to conduct risk-reduction investigations involving the coordinated motion of multiple satellites in a micro-gravity environment.

a Statistical Analysis on the System Performance of a Bluetooth Low Energy Indoor Positioning System in a 3d Environment

NASA Astrophysics Data System (ADS)

Haagmans, G. G.; Verhagen, S.; Voûte, R. L.; Verbree, E.

2017-09-01

Since GPS tends to fail for indoor positioning purposes, alternative methods like indoor positioning systems (IPS) based on Bluetooth low energy (BLE) are developing rapidly. Generally, IPS are deployed in environments covered with obstacles such as furniture, walls, people and electronics influencing the signal propagation. The major factor influencing the system performance and to acquire optimal positioning results is the geometry of the beacons. The geometry of the beacons is limited to the available infrastructure that can be deployed (number of beacons, basestations and tags), which leads to the following challenge: Given a limited number of beacons, where should they be placed in a specified indoor environment, such that the geometry contributes to optimal positioning results? This paper aims to propose a statistical model that is able to select the optimal configuration that satisfies the user requirements in terms of precision. The model requires the definition of a chosen 3D space (in our case 7 × 10 × 6 meter), number of beacons, possible user tag locations and a performance threshold (e.g. required precision). For any given set of beacon and receiver locations, the precision, internal- and external reliability can be determined on forehand. As validation, the modeled precision has been compared with observed precision results. The measurements have been performed with an IPS of BlooLoc at a chosen set of user tag locations for a given geometric configuration. Eventually, the model is able to select the optimal geometric configuration out of millions of possible configurations based on a performance threshold (e.g. required precision).

[Molecular beacon based PNA-FISH method combined with fluorescence scanning for rapid detection of Listeria monocytogenes].

PubMed

Wu, Shan; Zhang, Xiaofeng; Shuai, Jiangbing; Li, Ke; Yu, Huizhen; Jin, Chenchen

2016-07-04

To simplify the PNA-FISH (Peptide nucleic acid-fluorescence in situ hybridization) test, molecular beacon based PNA probe combined with fluorescence scanning detection technology was applied to replace the original microscope observation to detect Listeria monocytogenes The 5′ end and 3′ end of the L. monocytogenes specific PNA probes were labeled with the fluorescent group and the quenching group respectively, to form a molecular beacon based PNA probe. When PNA probe used for fluorescence scanning and N1 treatment as the control, the false positive rate was 11.4%, and the false negative rate was 0; when N2 treatment as the control, the false positive rate decreased to 4.3%, but the false negative rate rose to 18.6%. When beacon based PNA probe used for fluorescence scanning, taken N1 treatment as blank control, the false positive rate was 8.6%, and the false negative rate was 1.4%; taken N2 treatment as blank control, the false positive rate was 5.7%, and the false negative rate was 1.4%. Compared with PNA probe, molecular beacon based PNA probe can effectively reduce false positives and false negatives. The success rates of hybridization of the two PNA probes were 83.3% and 95.2% respectively; and the rates of the two beacon based PNA probes were 91.7% and 90.5% respectively, which indicated that labeling the both ends of the PNA probe dose not decrease the hybridization rate with the target bacteria. The combination of liquid phase PNA-FISH and fluorescence scanning method, can significantly improve the detection efficiency.

Low cost high efficiency GaAs monolithic RF module for SARSAT distress beacons

NASA Technical Reports Server (NTRS)

Petersen, W. C.; Siu, D. P.; Cook, H. F.

1991-01-01

Low cost high performance (5 Watts output) 406 MHz beacons are urgently needed to realize the maximum utilization of the Search and Rescue Satellite-Aided Tracking (SARSAT) system spearheaded in the U.S. by NASA. Although current technology can produce beacons meeting the output power requirement, power consumption is high due to the low efficiency of available transmitters. Field performance is currently unsatisfactory due to the lack of safe and reliable high density batteries capable of operation at -40 C. Low cost production is also a crucial but elusive requirement for the ultimate wide scale utilization of this system. Microwave Monolithics Incorporated (MMInc.) has proposed to make both the technical and cost goals for the SARSAT beacon attainable by developing a monolithic GaAs chip set for the RF module. This chip set consists of a high efficiency power amplifier and a bi-phase modulator. In addition to implementing the RF module in Monolithic Microwave Integrated Circuit (MMIC) form to minimize ultimate production costs, the power amplifier has a power-added efficiency nearly twice that attained with current commercial technology. A distress beacon built using this RF module chip set will be significantly smaller in size and lighter in weight due to a smaller battery requirement, since the 406 MHz signal source and the digital controller have far lower power consumption compared to the 5 watt power amplifier. All the program tasks have been successfully completed. The GaAs MMIC RF module chip set has been designed to be compatible with the present 406 MHz signal source and digital controller. A complete high performance low cost SARSAT beacon can be realized with only additional minor iteration and systems integration.

Pedestrian hybrid beacon crosswalk system (PHB) or high-intensity activated crosswalk (HAWK).

DOT National Transportation Integrated Search

2014-11-01

The Pedestrian Hybrid Beacon Crosswalk (PHB) is a type of traffic control system, used to aid : pedestrians safely crossing the street and to regulate traffic flow. This study examines the : success of the first PHB installed in the state of Vermont....

Conceptual development of a ground-based radio-beacon navigation system for use on the surface of the moon

NASA Technical Reports Server (NTRS)

Beggins, Andrew J.; Canney, Lora M.; Dolezal, Anna Belle

1988-01-01

A spread-spectrum radio-beacon navigation system for use on the lunar surface is described. The subjects discussed are principle of operation and specifications to include power requirements, operating frequencies, weight, size, and range.

Enhanced Terminal Information Services (ETIS) Utilizing the Discrete Address Beacon System (DABS) Data Link - Concept Description

DOT National Transportation Integrated Search

1979-07-01

This report describes a concept for providing enhanced terminal information services (ETIS) to aircraft utilizing the ground-air-ground data link capability of the Discrete Address Beacon System (DABS). ETIS is envisioned as an eventual replacement f...

Radiometer calibration procedure and beacon attenuation estimation reference level

NASA Technical Reports Server (NTRS)

Crane, Robert K.

1994-01-01

The primary objectives are to compare radiometer attenuation with beacon attenuation and to compare sky temperature estimates with calculations using simultaneous meteorological data. Secondary objectives are: (1) noise diode and reference load measurements and (2) to adjust for outside temperature and component temperature changes.

14 CFR 171.207 - Performance requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Performance requirements. 171.207 Section...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES VHF Marker Beacons § 171.207 Performance requirements. (a) VHF Marker Beacons must meet the performance requirements set forth in the “International...

14 CFR 171.207 - Performance requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Performance requirements. 171.207 Section...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES VHF Marker Beacons § 171.207 Performance requirements. (a) VHF Marker Beacons must meet the performance requirements set forth in the “International...

14 CFR 171.207 - Performance requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Performance requirements. 171.207 Section...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES VHF Marker Beacons § 171.207 Performance requirements. (a) VHF Marker Beacons must meet the performance requirements set forth in the “International...

Experimental evaluation of open-loop UpLink Power Control using ACTS

NASA Technical Reports Server (NTRS)

Dissanayake, Asoka

1995-01-01

The present investigation deals with the implementation of open-loop up-link power control using a beacon signal in the down-link frequency band as the control parameter. A power control system was developed and tested using the ACTS satellite. ACTS carries beacon signals in both up- and down-link bands with which the relationship between the up- and down-link fading can be established. A power controlled carrier was transmitted to the ACTS satellite from a NASA operated ground station and the transponded signal was received at COMSAT Laboratories using a terminal that was routinely used to monitor the two ACTS beacon signals. The experiment ran for a period of approximately six months and the collected data were used to evaluate the performance of the power control system. A brief review of propagation factors involved in estimating the up-link fade using a beacon signal in the down-link band are presented. The power controller design and the experiment configuration are discussed. Results of the experiment are discussed.

HRO: A New Forward-Scatter Observation Method Using a Ham-Band Beacon

NASA Astrophysics Data System (ADS)

Maegawa, K.

1999-02-01

A new forward-scatter meteor observation method has been used since 1996 in Japan. It uses its own 50 W continuous wave beacon with a broad directivity antenna on 53.750 MHz. To compensate for the weak echo power from the beacon, observers use SSB mode receivers and narrow band echo detection methods with Fast Fourier Transform software on personal computers. More than 250000 echoes have been counted per year so far. >From these results, diurnal and seasonal variations have been derived and are presented and discussed here. This method (HRO) will continue to play a leading radio observation role in Japan for the future.

Controller-reported performance defects in the air traffic control radar beacon system (1971 survey)

DOT National Transportation Integrated Search

1973-03-01

This report analyzes the returns from a recent ATC performance survey initiated by the Beacon System Interference Problem Subgroup. The survey began on the 27 November 1971 and lasted for two weeks. Participatione was limited to 37 facilities with pr...

Analysis of space shuttle main engine data using Beacon-based exception analysis for multi-missions

NASA Technical Reports Server (NTRS)

Park, H.; Mackey, R.; James, M.; Zak, M.; Kynard, M.; Sebghati, J.; Greene, W.

2002-01-01

This paper describes analysis of the Space Shuttle Main Engine (SSME) sensor data using Beacon-based exception analysis for multimissions (BEAM), a new technology developed for sensor analysis and diagnostics in autonomous space systems by the Jet Propulsion Laboratory (JPL).

X/X/Ka-band prime focus feed antenna for the Mars Observer beacon spacecraft

NASA Technical Reports Server (NTRS)

Stanton, P.; Reilly, H.; Esquivel, M.

1988-01-01

The results of an X/X/Ka-band feed design concept demonstration are presented. The purpose is to show the feasibility of adding a Ka-band beacon to the Mars Observer spacecraft. Scale model radiation patterns were made and analyzed.

46 CFR 25.26-5 - Commercial fishing industry vessels.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Commercial fishing industry vessels. 25.26-5 Section 25... Position Indicating Radio Beacons (EPIRB) § 25.26-5 Commercial fishing industry vessels. (a) The owner of a fishing vessel, a fish processing vessel, or a fish tender vessel, 11 meters (36 feet) or more in length...

46 CFR 25.26-5 - Commercial fishing industry vessels.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Commercial fishing industry vessels. 25.26-5 Section 25... Position Indicating Radio Beacons (EPIRB) § 25.26-5 Commercial fishing industry vessels. (a) The owner of a fishing vessel, a fish processing vessel, or a fish tender vessel, 11 meters (36 feet) or more in length...

46 CFR 25.26-5 - Commercial fishing industry vessels.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Commercial fishing industry vessels. 25.26-5 Section 25... Position Indicating Radio Beacons (EPIRB) § 25.26-5 Commercial fishing industry vessels. (a) The owner of a fishing vessel, a fish processing vessel, or a fish tender vessel, 11 meters (36 feet) or more in length...

46 CFR 25.26-5 - Commercial fishing industry vessels.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Commercial fishing industry vessels. 25.26-5 Section 25... Position Indicating Radio Beacons (EPIRB) § 25.26-5 Commercial fishing industry vessels. (a) The owner of a fishing vessel, a fish processing vessel, or a fish tender vessel, 11 meters (36 feet) or more in length...

46 CFR 25.26-5 - Commercial fishing industry vessels.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Commercial fishing industry vessels. 25.26-5 Section 25... Position Indicating Radio Beacons (EPIRB) § 25.26-5 Commercial fishing industry vessels. (a) The owner of a fishing vessel, a fish processing vessel, or a fish tender vessel, 11 meters (36 feet) or more in length...

Submarine harbor navigation using image data

NASA Astrophysics Data System (ADS)

Stubberud, Stephen C.; Kramer, Kathleen A.

2017-01-01

The process of ingress and egress of a United States Navy submarine is a human-intensive process that takes numerous individuals to monitor locations and for hazards. Sailors pass vocal information to bridge where it is processed manually. There is interest in using video imaging of the periscope view to more automatically provide navigation within harbors and other points of ingress and egress. In this paper, video-based navigation is examined as a target-tracking problem. While some image-processing methods claim to provide range information, the moving platform problem and weather concerns, such as fog, reduce the effectiveness of these range estimates. The video-navigation problem then becomes an angle-only tracking problem. Angle-only tracking is known to be fraught with difficulties, due to the fact that the unobservable space is not the null space. When using a Kalman filter estimator to perform the tracking, significant errors arise which could endanger the submarine. This work analyzes the performance of the Kalman filter when angle-only measurements are used to provide the target tracks. This paper addresses estimation unobservability and the minimal set of requirements that are needed to address it in this complex but real-world problem. Three major issues are addressed: the knowledge of navigation beacons/landmarks' locations, the minimal number of these beacons needed to maintain the course, and update rates of the angles of the landmarks as the periscope rotates and landmarks become obscured due to blockage and weather. The goal is to address the problem of navigation to and from the docks, while maintaining the traversing of the harbor channel based on maritime rules relying solely on the image-based data. The minimal number of beacons will be considered. For this effort, the image correlation from frame to frame is assumed to be achieved perfectly. Variation in the update rates and the dropping of data due to rotation and obscuration is considered. The analysis will be based on a simple straight-line channel harbor entry to the dock, similar to a submarine entering the submarine port in San Diego.

Promoting Health in Hospitals: The Role of Beacon

ERIC Educational Resources Information Center

Whitelaw, Sandy; Watson, Jonathan; Hennessy, Sue

2004-01-01

"Best practice" is currently being used to enable modernisation within the National Health Service (NHS) in the United Kingdom. One element of this is the Beacon programme where examples of hospitals that exemplify local "best practice" are supported to develop and disseminate learning across the wider NHS. The aims of this…

Beacon Spacecraft Operations: Lessons in Automation

NASA Technical Reports Server (NTRS)

Sherwood, R.; Schlutsmeyer, A.; Sue, M.; Szijjarto, J.; Wyatt, E. J.

2000-01-01

A new approach to mission operations has been flight validated on NASA's Deep Space One (DS1) mission that launched in October 1998. The beacon monitor operations technology is aimed at decreasing the total volume of downlinked engineering telemetry by reducing the frequency of downlink and the volume of data received per pass.

Evaluation of a rectangular rapid flashing beacon system at the Belmont Ridge Road and W&OD Trail mid-block crosswalk.

DOT National Transportation Integrated Search

2015-05-01

On April 8, 2013, the Virginia Department of Transportation (VDOT) installed a Rectangular Rapid Flashing Beacon : (RRFB) system at Belmont Ridge Road in Loudoun County that included two units at the Washington and Old Dominion : (W&OD) Trail crossin...

47 CFR 80.605 - U.S. Coast Guard coordination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... documentation as to this fact. Note: Surveillance radar coast stations do not require U.S. Coast Guard approval. (b) Coast station transponders (i.e., radar beacons, or racons) operating in the band 2900-3100 or... Radar Beacons (RACONS),” with Annexes, 1995. Applications for certification of these transponders must...

47 CFR 80.605 - U.S. Coast Guard coordination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... documentation as to this fact. Note: Surveillance radar coast stations do not require U.S. Coast Guard approval. (b) Coast station transponders (i.e., radar beacons, or racons) operating in the band 2900-3100 or... Radar Beacons (RACONS),” with Annexes, 1995. Applications for certification of these transponders must...

Locate and rescue system components

NASA Technical Reports Server (NTRS)

1976-01-01

Two types of search and rescue systems not involving satellites are studied; one using a network radio communications link and the other a characteristic beacon signal. Line of slight limitation of VHF radio and beacon signals limit the range (approximately 25 miles) between the origin of the distress signal and the mobile rescue unit.

76 FR 17625 - Proposed Information Collection; Comment Request; Emergency Beacon Registrations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-30

... Collection; Comment Request; Emergency Beacon Registrations AGENCY: National Oceanic and Atmospheric... satellites to detect and locate ships, aircraft, or individuals in distress if they are equipped with an... provided by registration can assist in identifying who is in trouble and in suppressing false alarms. II...

76 FR 36899 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-23

...: National Oceanic and Atmospheric Administration (NOAA). Title: Emergency Beacon Registrations. OMB Control..., aircraft, or individuals in distress if they are equipped with an emergency radio beacon. Persons...) regulations at 47 CFR 80.1061, 47 CFR 87.199 and 47 CFR 95.1402. The data provided by registration can assist...

Automated Internet-Based Control of Spacecraft Groundstations: Beacon-Based Health Monitoring Concept

NASA Technical Reports Server (NTRS)

Cantwell, Brian; Twiggs, Robert; Swartwout, Michael

1997-01-01

This report serves as an update about the activities of Stanford University's Space Systems Development Laboratory (SSDL) in their beacon-based health monitoring experiment. Section 1 describes the goals of the project and the organization of the team. Section 2 provides an overview of the major components of the system, describing the general approach of automated health monitoring and the beacon signal relay. It also provides background about the SAPPHIRE spacecraft and ASSET operations system, which will be used for the experiment. Specific details about implementation and status of each element of the experiment are found in Section 3. Section 4 describes the experiment and future work, and references are contained in Section 5.

Long Shelf Life of a Lyophilized DNA Aptamer Beacon Assay.

PubMed

Bruno, John G

2017-03-01

An aptamer beacon previously developed to detect C-telopeptide (CTx) from human bone collagen breakdown was lyophilized and shown to give a "lights on" concentration-dependent spectral fluorescence response essentially identical to that of the fresh reagent despite storage in a dark dry environment for the past 5.5 years.

Design of a hybrid receiver for the OLYMPUS spacecraft beacons

NASA Technical Reports Server (NTRS)

Sweeney, D. G.; Mckeeman, J. C.

1990-01-01

The theory and design of a hybrid analogue/digital receiver which acquires and monitors the OLYMPUS satellite beacons is presented. The analogue portion of this receiver uses a frequency locked loop for signal tracking. A digital sampling detector operating at IF is used to obtain the I and Q outputs.

COSPAS/SARSAT 406-MHz emergency beacon digital controller

NASA Technical Reports Server (NTRS)

Ivancic, William D.

1988-01-01

The digital control portion of a low-cost 406-MHz COSPAS/SARSAT emergency beacon has been designed and breadboarded at the NASA Lewis Research Center. This report discusses the requirements and design tradeoffs of the digital controller and describes the hardware and software design, which is available only to United States citizens and companies.

The Effects of a Rectangular Rapid-Flashing Beacon on Vehicle Speed

ERIC Educational Resources Information Center

VanWagner, Michelle; Van Houten, Ron; Betts, Brian

2011-01-01

In 2008, nearly 31% of vehicle fatalities were related to failure to adhere to safe vehicle speeds (National Highway Traffic Safety Administration [NHTSA], 2009). The current study evaluated the effect of a rectangular rapid-flashing beacon (RRFB) triggered by excessive speed on vehicle speed using a combined alternating treatments and reversal…

Impact of beacon wavelength on phase-compensation performance

NASA Astrophysics Data System (ADS)

Enterline, Allison A.; Spencer, Mark F.; Burrell, Derek J.; Brennan, Terry J.

2017-09-01

This study evaluates the effects of beacon-wavelength mismatch on phase-compensation performance. In general, beacon-wavelength mismatch occurs at the system level because the beacon-illuminator laser (BIL) and high-energy laser (HEL) are often at different wavelengths. Such is the case, for example, when using an aperture sharing element to isolate the beam-control sensor suite from the blinding nature of the HEL. With that said, this study uses the WavePlex Toolbox in MATLAB® to model ideal spherical wave propagation through various atmospheric-turbulence conditions. To quantify phase-compensation performance, we also model a nominal adaptive-optics (AO) system. We achieve correction from a Shack-Hartmann wavefront sensor and continuous-face-sheet deformable mirror using a least-squares phase reconstruction algorithm in the Fried geometry and a leaky integrator control law. To this end, we plot the power in the bucket metric as a function of BIL-HEL wavelength difference. Our initial results show that positive BIL-HEL wavelength differences achieve better phase compensation performance compared to negative BIL-HEL wavelength differences (i.e., red BILs outperform blue BILs). This outcome is consistent with past results.

Vibration control for the ARGOS laser launch path

NASA Astrophysics Data System (ADS)

Peter, Diethard; Gässler, Wolfgang; Borelli, Jose; Barl, Lothar; Rabien, S.

2012-07-01

Present and future adaptive optics systems aim for the correction of the atmospheric turbulence over a large field of view combined with large sky coverage. To achieve this goal the telescope is equipped with multiple laser beacons. Still, to measure tip-tilt aberrations a natural guide star is used. For some fields such a tilt-star is not available and a correction on the laser beacons alone is applied. For this method to work well the laser beacons must not be affected by telescope vibrations on their up-link path. For the ARGOS system the jitter of the beacons is specified to be below 0.05. To achieve this goal a vibration compensation system is necessary to mitigate the mechanical disturbances. The ARGOS vibration compensation system is an accelerometer based feed forward system. The accelerometer measurements are fed into a real time controller. To achieve high performance the controller of the system is model based. The output is applied to a fast steering mirror. This paper presents the concept of the ARGOS vibration compensation, the hardware, and laboratory results.

The trillion planet survey: an optical search for directed intelligence in M31

NASA Astrophysics Data System (ADS)

Stewart, Andrew; Lubin, Philip

2017-09-01

In realm of optical SETI, searches for pulsed laser signals have historically been preferred over those for continuous wave beacons. There are many valid reasons for this, namely the near elimination of false positives and simple experimental components. However, due to significant improvements in laser technologies and light-detection systems since the mid-20th century, as well as new data from the recent Kepler mission, continuous wave searches should no longer be ignored. In this paper we propose a search for continuous wave laser beacons from an intelligent civilization in the Andromeda galaxy. Using only a 0.8 meter telescope, a standard photometric system, and an image processing pipeline, we expect to be able to detect any CW laser signal directed at us from an extraterrestrial civilization in M31, as long as the civilization is operating at a wavelength we can "see" and has left the beacon on long enough for us to detect it here on Earth. The search target is M31 due to its high stellar density relative to our own Milky Way galaxy. Andromeda is home to at least one trillion stars, and thus at least one trillion planets. As a result, in surveying M31, we are surveying one trillion planets, and consequently one trillion possible locations of intelligent life. This is an unprecedented number of targets relative to other past SETI searches. We call this the TPS or Trillion Planet Survey.

Beacon Hill end moraine, Boston: new explanation of an important urban feature

USGS Publications Warehouse

Kaye, Clifford A.; Coates, Donald R.

1976-01-01

The usefulness of geology to engineers is in direct proportion to how well it helps us predict the subsurface; these predictions, in turn, depend on our knowledge of the geomorphic processes that molded the terrain. The uncertainties of interpretation are particularly great in glaciated terrain because our understanding of both glacial processes and history is so incomplete, a fact well illustrated in Beacon Hill. Recent construction activities in the eastern part of the hill, until now classified as a drumlin, have shown that it is better interpreted as an end moraine formed by a Wisconsonian glacial readvance. Instead of the firm till that was anticipated as foundation material, excavations exposed a complex of sand, gravel, and clay, with only minor zones of till. The structure of these deposits strongly suggests that originally they were plates of the glacial bed that froze to the glacier and were transported englacially. Thrust faulting and other deformations are glacial structures formed within the ice in the glacier's terminal zone. In spite of the complex englacial history, these deposits lost little of their original appearance and intergranular relationships. Upon deglaciation, the frozen moraine thawed, and slumping formed complex secondary structures on the ridge's lower flanks.

Investigation and Development of Data-Driven D-Region Model for HF Systems Impacts

NASA Technical Reports Server (NTRS)

Eccles, J. V.; Rice, D.; Sojka, J. J.; Hunsucker, R. D.

2002-01-01

Space Environment Corporation (SEC) and RP Consultants (RPC) are to develop and validate a weather-capable D region model for making High Frequency (HF) absorption predictions in support of the HF communications and radar communities. The weather-capable model will assimilate solar and earth space observations from NASA satellites. The model will account for solar-induced impacts on HF absorption, including X-rays, Solar Proton Events (SPE's), and auroral precipitation. The work plan includes: I . Optimize D-region model to quickly obtain ion and electron densities for proper HF absorption calculations. 2. Develop indices-driven modules for D-region ionization sources for low, mid, & high latitudes including X-rays, cosmic rays, auroral precipitation, & solar protons. (Note: solar spectrum & auroral modules already exist). 3. Setup low-cost monitors of existing HF beacons and add one single-frequency beacon. 4. Use PENEX HF-link database with HF monitor data to validate D-region/HF absorption model using climatological ionization drivers. 5. Develop algorithms to assimilate NASA satellite data of solar, interplanetary, and auroral observations into ionization source modules. 6. Use PENEX HF-link & HF-beacon data for skill score comparison of assimilation versus climatological D-region/HF absorption model. Only some satellites are available for the PENEX time period, thus, HF-beacon data is necessary. 7. Use HF beacon monitors to develop HF-link data assimilation algorithms for regional improvement to the D-region/HF absorption model.

A molecular-beacon-based asymmetric PCR assay for easy visualization of amplicons in the diagnosis of trichomoniasis.

PubMed

Sonkar, Subash C; Sachdev, Divya; Mishra, Prashant K; Kumar, Anita; Mittal, Pratima; Saluja, Daman

2016-12-15

The currently available nucleic acid amplification tests (NAATs) for trichomoniasis are accurate, quick and confirmative with superior sensitivity than traditional culture-based microbiology assays. However, these assays are associated with problems of carry over contamination, false positive results, requirement of technical expertise for performance and detection of end product. Hence, a diagnostic assay with easy visualization of the amplified product will be profitable. An in-house, rapid, sensitive, specific molecular-beacon-based PCR assay, using primers against pfoB gene of Trichomonas vaginalis, was developed and evaluated using dry ectocervical swabs (n=392) from symptomatic females with vaginal discharge. Total DNA was isolated and used as template for the PCR assays. The performance and reproducibility of PCR assay was evaluated by composite reference standard (CRS). For easy visualization of the amplified product, molecular-beacon was designed and amplicons were visualized directly using fluorescent handheld dark reader or by Micro-Plate Reader. Molecular-beacons are single-stranded hairpin shaped nucleic acid probes composed of a stem, with fluorophore/quencher pair and a loop region complementary to the desired DNA. The beacon-based PCR assay designed in the present study is highly specific as confirmed by competition experiments and extremely sensitive with detection limit of 20fg of genomic DNA (3-4 pathogens). The minimum infrastructure requirement and ease to perform the assay makes this method highly useful for resource poor countries for better disease management. Copyright © 2016 Elsevier B.V. All rights reserved.

Data governance and data sharing agreements for community-wide health information exchange: lessons from the beacon communities.

PubMed

Allen, Claudia; Des Jardins, Terrisca R; Heider, Arvela; Lyman, Kristin A; McWilliams, Lee; Rein, Alison L; Schachter, Abigail A; Singh, Ranjit; Sorondo, Barbara; Topper, Joan; Turske, Scott A

2014-01-01

Unprecedented efforts are underway across the United States to electronically capture and exchange health information to improve health care and population health, and reduce costs. This increased collection and sharing of electronic patient data raises several governance issues, including privacy, security, liability, and market competition. Those engaged in such efforts have had to develop data sharing agreements (DSAs) among entities involved in information exchange, many of whom are "nontraditional" health care entities and/or new partners. This paper shares lessons learned based on the experiences of six federally funded communities participating in the Beacon Community Cooperative Agreement Program, and offers guidance for navigating data governance issues and developing DSAs to facilitate community-wide health information exchange. While all entities involved in electronic data sharing must address governance issues and create DSAs accordingly, until recently little formal guidance existed for doing so - particularly for community-based initiatives. Despite this lack of guidance, together the Beacon Communities' experiences highlight promising strategies for navigating complex governance issues, which may be useful to other entities or communities initiating information exchange efforts to support delivery system transformation. For the past three years, AcademyHealth has provided technical assistance to most of the 17 Beacon Communities, 6 of whom contributed to this collaborative writing effort. Though these communities varied widely in terms of their demographics, resources, and Beacon-driven priorities, common themes emerged as they described their approaches to data governance and DSA development. The 6 Beacon Communities confirmed that DSAs are necessary to satisfy legal and market-based concerns, and they identified several specific issues, many of which have been noted by others involved in network data sharing initiatives. More importantly, these communities identified several promising approaches to timely and effective DSA development, including: stakeholder engagement; identification and effective communication of value; adoption of a parsimonious approach; attention to market-based concerns; flexibility in adapting and expanding existing agreements and partnerships; and anticipation of required time and investment.

The Evolution of Hydrocarbon Compounds in Saturn's Stratosphere During the 2010 Northern Storm

NASA Astrophysics Data System (ADS)

Hesman, B. E.; Bjoraker, G. L.; Achterberg, R. K.; Sada, P. V.; Jennings, D. E.; Lunsford, A. W.; Sinclair, J.; Romani, P. N.; Boyle, R.; Fletcher, L. N.; Irwin, P.

2013-12-01

The massive eruption at 40N (planetographic latitude) in December 2010 has produced significant and long-lived changes in temperature and species abundances in Saturn's northern hemisphere (Hesman et al. 2012a, Fletcher et al. 2012). The northern storm region has been observed on many occasions between January 2011 and June of 2012 by Cassini's Composite Infrared Spectrometer (CIRS). In this time period, temperatures in regions referred to as 'beacons' (warm regions in the stratosphere at certain longitudes in the storm latitude) became significantly warmer than pre-storm values of 140K. In this period hydrocarbon emission greatly increased; however, this increased emission could not be attributed due to the temperature changes alone for many of these species (Hesman et al. 2012b, Bjoraker et al 2012). The unique nature of the stratospheric beacons also resulted in the detection of ethylene (C2H4) using CIRS. These beacon regions have also led to the identification of rare hydrocarbon species such as C4H2 and C3H4 in the stratosphere. These species are all expected from photochemical processes in the stratosphere, however high temperatures, unusual chemistry, or dynamics are enhancing these species. The exact cause of these enhancements is still under investigation. Ground-based observations were performed using the high-resolution spectrometer Celeste in May 2011 to confirm the CIRS detection of C2H4 and to study its spectral signatures at higher spectral resolution. In order to follow the evolution of its emission further observations were performed in July 2011 and March 2012. These observations are being used in conjunction with the CIRS observations to investigate the source of the approximately 100-fold increase of ethylene in the stratospheric beacon. The time evolution of hydrocarbon emission from C2H2, C2H4, C2H6, C3H4, and C4H2 in Saturn's Northern Storm beacon regions will be discussed. References: Bjoraker, G., B.E. Hesman, R.K. Achterberg, P.N. Romani. 2012, 'The Evolution of Hydrocarbons in Saturn's Northern Storm Region,' AAS DPS Conference, Vol. 44, #403.05. Fletcher, L.N. et al. 2012, 'The Origin and Evolution of Saturn's 2011-2012 Stratospheric Vortex,' Icarus, 221, 560-586. Hesman, B.E. et al. 2012a, 'Elusive Ethylene Detected in Saturn's Northern Storm Region,' The Astrophysical Journal, 760, 24-30. Hesman, B.E. et al. 2012b, 'Ethylene Emission in the Aftermath of Saturn's 2010 Northern Storm,' AAS DPS Conference, Vol. 44, #403.06.

The Methodist Hospital CCU: a Beacon unit of excellence.

PubMed

Lewis, Tricia; Abanobi, Beatrice; Alleman, Paula; Ballinas, Eva; Botbyl, Brenda; Bries, Girlie; Clark, Liz; Clarkson, Terri; Cooper, Bridgette; Cooper, Jason; Cox, Shelly; Cude, Rebecca; Davis, Juanita; Delosreyes, Arlene; Durst, Kimberly; East, Ana; Edwards, Erving; Ellison, Rose; Eparwa, Perlita; Forjuoh, Harriet; Foster, Donald; Foytik, Lisa; Gordon, Maria; Grace, Jean; Green, Lisa; Harrison, Cassandra; Herrera, Rubin; Horn, Mary; Hunsinger, Cheryce; Issac, Annamma; Jackson, Valerie; Johnson, Athie; Kitayama, Susanna; Karaan, Juliet; Kezmarski, Mary; Kleinrock, Richard; Latson, Maria; Lee, Valerie; Long, Robin; Macapagal, Fred; Magsino, Diana; Manojkumar, Saleena; Martin, Denise; Matura, Lea Ann; McCarthy, Cynthia; McClellan, Emma; McDaniel, Christy; Mengo, Pam; Meurer, Judy; Muttathottil, Liz; Osayande, Esther; Osei-Frimpong, Diana; Phillips, Betty; Pittman, James; Pratt, Craig; Putney, David; Refuerzo, Laarni; Rendon, Lavinia; Richter, Russ; Simms, Sylvia; Sitoy, Diana; Small-Nelson, Judith; Sosa, Tomas; Stuckey, Denise; Tacquard, Natalie; Talbott, Sarah; Talladen, Aleine; Taylor, Shani; Thomas, Lini; Thompson, Pam; Walters, Gordon; West, Rachel

2005-06-01

This article features the Coronary Care Unit of The Methodist Hospital of Houston, Texas. This unit was one of the first Beacon Critical Care Units recognized by the American Association of Critical Care Nurses. This article focuses on how to achieve this award. The Coronary Care Unit nursing infrastructure is described, and specific unit examples are included.

Guiding Math Students to Campus Services: An Impact Evaluation of the Beacon Program at South Texas College

ERIC Educational Resources Information Center

Visher, Mary; Butcher, Kristin F.; Cerna, Oscar S.

2011-01-01

This research rigorously evaluates whether a low-cost intervention can improve students' performance in developmental math. The "Beacon Mentoring Program" was developed at South Texas College by professors, administrators, and staff at the college. Surveys of students revealed that many did not have someone on campus whom they felt they…

Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs.

PubMed

Wile, Brian M; Ban, Kiwon; Yoon, Young-Sup; Bao, Gang

2014-10-01

Molecular beacons (MBs) are dual-labeled oligonucleotides that fluoresce only in the presence of complementary mRNA. The use of MBs to target specific mRNAs allows sorting of specific cells from a mixed cell population. In contrast to existing approaches that are limited by available surface markers or selectable metabolic characteristics, the MB-based method enables the isolation of a wide variety of cells. For example, the ability to purify specific cell types derived from pluripotent stem cells (PSCs) is important for basic research and therapeutics. In addition to providing a general protocol for MB design, validation and nucleofection into cells, we describe how to isolate a specific cell population from differentiating PSCs. By using this protocol, we have successfully isolated cardiomyocytes differentiated from mouse or human PSCs (hPSCs) with ∼ 97% purity, as confirmed by electrophysiology and immunocytochemistry. After designing MBs, their ordering and validation requires 2 weeks, and the isolation process requires 3 h.

VLC-beacon detection with an under-sampled ambient light sensor

NASA Astrophysics Data System (ADS)

Green, Jacob; Pérez-Olivas, Huetzin; Martínez-Díaz, Saúl; García-Márquez, Jorge; Domínguez-González, Carlos; Santiago-Montero, Raúl; Guan, Hongyu; Rozenblat, Marc; Topsu, Suat

2017-08-01

LEDs will replace in a near future the current worldwide lighting mainly due to their low production-cost and energy-saving assets. Visible light communications (VLC) will turn gradually the existing lighting network into a communication network. Nowadays VLC transceivers can be found in some commercial centres in Europe; some of them broadcast continuously an identification tag that contains its coordinate position. In such a case, the transceiver acts as a geolocation beacon. Nevertheless, mobile transceivers represent a challenge in the VLC communication chain, as smartphones have not integrated yet a VLC customized detection stage. In order to make current smartphones capable to detect VLC broadcasted signals, their Ambient Light Sensor (ALS) is adapted as a VLC detector. For this to be achieved, lighting transceivers need to adapt their modulation scheme. For instance, frequencies representing start bit, 1, and 0 logic values can be set to avoid flicker from illumination and to permit detecting the under-sampled signal. Decoding the signal requires a multiple steps real-time signal processing as shown here.

BErkeley Atmospheric CO2 Network (BEACON) - Bringing Measurements of CO2 Emissions to a School Near You

NASA Astrophysics Data System (ADS)

Teige, V. E.; Havel, E.; Patt, C.; Heber, E.; Cohen, R. C.

2011-12-01

The University of California at Berkeley in collaboration with the Chabot Space and Science Center describe a set of educational programs, workshops, and exhibits based on a multi-node greenhouse gas and air quality monitoring network being deployed over Oakland, California. Examining raw numerical data using highly engaging and effective geo-data visualization tools like Google Earth can make the science come alive for students, and provide a hook for drawing them into deeper investigations. The Climate Science Investigations teacher workshop at the Chabot Space and Science Center will make use of Google Earth, Excel, and other geo-data visualization tools to step students through the process from data acquisition to discovery. Using multiple data sources, including output from the BErkeley Atmospheric CO2 Network (BEACON) project, participants will be encouraged to explore a variety of different modes of data display toward producing a unique, and ideally insightful, illumination of the data.

Micro-machined resonator oscillator

DOEpatents

Koehler, Dale R.; Sniegowski, Jeffry J.; Bivens, Hugh M.; Wessendorf, Kurt O.

1994-01-01

A micro-miniature resonator-oscillator is disclosed. Due to the miniaturization of the resonator-oscillator, oscillation frequencies of one MHz and higher are utilized. A thickness-mode quartz resonator housed in a micro-machined silicon package and operated as a "telemetered sensor beacon" that is, a digital, self-powered, remote, parameter measuring-transmitter in the FM-band. The resonator design uses trapped energy principles and temperature dependence methodology through crystal orientation control, with operation in the 20-100 MHz range. High volume batch-processing manufacturing is utilized, with package and resonator assembly at the wafer level. Unique design features include squeeze-film damping for robust vibration and shock performance, capacitive coupling through micro-machined diaphragms allowing resonator excitation at the package exterior, circuit integration and extremely small (0.1 in. square) dimensioning. A family of micro-miniature sensor beacons is also disclosed with widespread applications as bio-medical sensors, vehicle status monitors and high-volume animal identification and health sensors. The sensor family allows measurement of temperatures, chemicals, acceleration and pressure. A microphone and clock realization is also available.

Basic Investigations of Dynamic Travel Time Estimation Model for Traffic Signals Control Using Information from Optical Beacons

NASA Astrophysics Data System (ADS)

Okutani, Iwao; Mitsui, Tatsuro; Nakada, Yusuke

In this paper put forward are neuron-type models, i.e., neural network model, wavelet neuron model and three layered wavelet neuron model(WV3), for estimating traveling time between signalized intersections in order to facilitate adaptive setting of traffic signal parameters such as green time and offset. Model validation tests using simulated data reveal that compared to other models, WV3 model works very fast in learning process and can produce more accurate estimates of travel time. Also, it is exhibited that up-link information obtainable from optical beacons, i.e., travel time observed during the former cycle time in this case, makes a crucial input variable to the models in that there isn't any substantial difference between the change of estimated and simulated travel time with the change of green time or offset when up-link information is employed as input while there appears big discrepancy between them when not employed.

Ultranarrowband searches for extraterrestrial intelligence with dedicated signal-processing hardware.

PubMed

Horowitz, P; Matthews, B S; Forster, J; Linscott, I; Teague, C C; Chen, K; Backus, P

1986-01-01

Multichannel spectroscopy with millihertz resolution constitutes an attractive strategy for a microwave search for extraterrestrial intelligence (SETI), assuming the transmission of a narrow-band radiofrequency beacon. Such resolution matches the properties of the interstellar medium, and the necessary receiver Doppler corrections provide a high degree of interference rejection. We have constructed a frequency-agile swept receiver with an 8,388,608-channel spectrum analyzer, on-line signal recognition, and multithreshold archiving. A search of 250 Sun-like stars at 1.4 and 2.8 GHz has been carried out with the Arecibo 305-m antenna, and a meridian transit search of the northern sky is in progress at the Harvard-Smithsonian 26-m antenna. Successive spectra of 400 kHz at 0.05 Hz resolution are searched for features characteristic of an intentional narrowband beacon transmission. These spectra are centered on guessable ("magic") frequencies (such as the 21-cm hydrogen hyperfine line), referenced successively to the local standard of rest, the galactic barycenter, and the cosmic blackbody rest frame.

Beacon of Hope: The Promise of Early Head Start for America's Youngest Children

ERIC Educational Resources Information Center

Lombardi, Joan, Ed.; Bogle, Mary M., Ed.

2005-01-01

Between 1995 and 2004, Early Head Start (EHS) grew from 68 to more than 700 programs nationwide, serving more than 62,000 children and securing the program's reputation as a beacon of hope for the nation's low-income families with infants and toddlers. According to a comprehensive 5-year national evaluation of EHS, children who participated in…

A NEW LOOK AT SPECIAL PROBLEMS, PROJECT BEACON--A PROJECT ADDRESSED TO THE NEEDS OF THE SOCIALLY DISADVANTAGED.

ERIC Educational Resources Information Center

FISHMAN, JOSHUA

A GREAT NUMBER OF AMERICAN CHILDREN ARE GROWING UP IN SOCIALLY DEPRIVED SURROUNDINGS. THESE DEPRIVATIONS ARE REFLECTED IN LOWER EDUCATIONAL ACHIEVEMENT. TEACHERS SHOULD HAVE SPECIAL PREPARATION IN ORDER TO LESSEN THIS WASTE OF EDUCATIONAL POTENTIAL. PROJECT BEACON IS AN EFFORT TO INTRODUCE WITHIN THE PUBLIC SCHOOL SYSTEM A PERMANENT CORPS OF…

75 FR 20364 - Public Buildings Service; Key Largo Beacon Annex Site; Key Largo, FL; Transfer of Property

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-19

... GENERAL SERVICES ADMINISTRATION [Wildlife Order 187; 4-D-FL-1218] Public Buildings Service; Key Largo Beacon Annex Site; Key Largo, FL; Transfer of Property Pursuant to section 2 of Public Law 537, 80th Congress, approved May 19, 1948 (16 U.S.C. 667c), notice is hereby given that: 1. The General...

The Child Development Training Consortium. A Status Report on the San Juan College AACJC-Kellogg Beacon College Project.

ERIC Educational Resources Information Center

Beers, C. David; Ott, Richard W.

The Child Development Training Consortium, a Beacon College Project directed by San Juan College (SJC) is a collaborative effort of colleges and universities in New Mexico and Arizona. The consortium's major objective is to create child development training materials for community college faculty who teach "at-risk" Native American and…

Beacon Charter School Needs a School: A Case of Capital Outlay of Charters in a Public District

ERIC Educational Resources Information Center

Wronowski, Meredith L.

2017-01-01

The case study presented below is a representation of a real-world, ongoing situation involving a public school district's capital outlay for charter schools within its boundaries. One particular charter, Beacon Charter School, was promised a permanent building by the public school district that also acts as its authorizer. However, recent events…

The New York City Beacons: Rebuilding Communities of Support in Urban Neighborhoods

ERIC Educational Resources Information Center

Kleinbard, Peter

2005-01-01

The first ten New York City Beacons began operation in 1991 as a result of the recommendation of a commission appointed by then-mayor David N. Dinkins. Richard Murphy, commissioner of youth services, led the development and implementation. Michele Cahill served as a consultant in the design and created the Youth Development Institute (YDI) as part…

Biomolecular Architectures Molecular Biology

DTIC Science & Technology

2013-08-31

when the Salmonella beacon (13 nM) was tested in the presence of 800 ng bacterial genomic DNA in chicken broth (33%) (data not shown). Since it was...bacterium, Bacillus thuringiensis, transgenic tobacco containing the transgene, Bt cry1Ac, the Gram-negative bacterium, Salmonella Typhimurium, and the Gram... Salmonella Typhimurium, and the Gram-positive bacterium, Listeria monocytogenes, were monitored for detection by coupling molecular beacon (MB

A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.

PubMed

Zhang, Feng; Li, Shuangming; Cao, Kang; Wang, Pengjuan; Su, Yan; Zhu, Xinhua; Wan, Ying

2015-06-11

A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the "aptamer beacon", highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

Fixation light hue bias revisited: implications for using adaptive optics to study color vision.

PubMed

Hofer, H J; Blaschke, J; Patolia, J; Koenig, D E

2012-03-01

Current vision science adaptive optics systems use near infrared wavefront sensor 'beacons' that appear as red spots in the visual field. Colored fixation targets are known to influence the perceived color of macroscopic visual stimuli (Jameson, D., & Hurvich, L. M. (1967). Fixation-light bias: An unwanted by-product of fixation control. Vision Research, 7, 805-809.), suggesting that the wavefront sensor beacon may also influence perceived color for stimuli displayed with adaptive optics. Despite its importance for proper interpretation of adaptive optics experiments on the fine scale interaction of the retinal mosaic and spatial and color vision, this potential bias has not yet been quantified or addressed. Here we measure the impact of the wavefront sensor beacon on color appearance for dim, monochromatic point sources in five subjects. The presence of the beacon altered color reports both when used as a fixation target as well as when displaced in the visual field with a chromatically neutral fixation target. This influence must be taken into account when interpreting previous experiments and new methods of adaptive correction should be used in future experiments using adaptive optics to study color. Copyright © 2012 Elsevier Ltd. All rights reserved.

Multi-Band Multi-Tone Tunable Millimeter-Wave Frequency Synthesizer For Satellite Beacon Transmitter

NASA Technical Reports Server (NTRS)

Simons, Rainee N.; Wintucky, Edwin G.

2016-01-01

This paper presents the design and test results of a multi-band multi-tone tunable millimeter-wave frequency synthesizer, based on a solid-state frequency comb generator. The intended application of the synthesizer is in a satellite beacon transmitter for radio wave propagation studies at K-band (18 to 26.5 GHz), Q-band (37 to 42 GHz), and E-band (71 to 76 GHz). In addition, the architecture for a compact beacon transmitter, which includes the multi-tone synthesizer, polarizer, horn antenna, and power/control electronics, has been investigated for a notional space-to-ground radio wave propagation experiment payload on a small satellite. The above studies would enable the design of robust high throughput multi-Gbps data rate future space-to-ground satellite communication links.

Personal Beacon

NASA Technical Reports Server (NTRS)

2000-01-01

The MicroPLB (personal locator beacon) is a search and rescue satellite-aided tracking (SARSAT) transmitter. When activated it emits a distress signal to a constellation of internationally operated satellites. The endangered person's identity and location anywhere on Earth is automatically forwarded to central monitoring stations around the world. It is accurate to within just a few meters. The user uses the device to download navigation data from a global positioning satellite receiver. After the download is complete, the MicroPLB functions as a self-locating beacon. Also, it is the only PLB to use a safe battery. In the past, other PLB devices have used batteries that have enough volatility to explode with extreme force. It was developed by Microwave Monolithic, Inc. through SBIR funding from Glenn Research Center and Goddard Space Flight Center.

Mobile robot trajectory tracking using noisy RSS measurements: an RFID approach.

PubMed

Miah, M Suruz; Gueaieb, Wail

2014-03-01

Most RF beacons-based mobile robot navigation techniques rely on approximating line-of-sight (LOS) distances between the beacons and the robot. This is mostly performed using the robot's received signal strength (RSS) measurements from the beacons. However, accurate mapping between the RSS measurements and the LOS distance is almost impossible to achieve in reverberant environments. This paper presents a partially-observed feedback controller for a wheeled mobile robot where the feedback signal is in the form of noisy RSS measurements emitted from radio frequency identification (RFID) tags. The proposed controller requires neither an accurate mapping between the LOS distance and the RSS measurements, nor the linearization of the robot model. The controller performance is demonstrated through numerical simulations and real-time experiments. ©2013 Published by ISA. All rights reserved.

Proceedings of the Symposium of the COSPAR Satellite Beacon Group on the Geophysical Use of Satellite Beacon Observations Held at Boston University on 1- 4 June 1976

DTIC Science & Technology

1976-06-04

CAL, , ,-"E - ’ :’’, EO..... : Ao GAAOEHO - - - 2 ’£ - - o- ,/ .""’ CO / 2: i i ; ’ o ,* , - /% t, ’ 2. 0 4 H 20 22 2 LOC AL’’E HL I Figure 7 Figure 8...24 0 4 S 2 LOC ~~lL TIMEASAI LOCAL T0k (bt) Figure 9 Figure 10 (233 1241 (252 1307 1334 1441 k L T 2526 2501 2449 23 76 2261 2159 AL 22 29 40 &o 105...981-1206, 1975 [2] Barrett, L. OTropospheric irregularity effects on the 360 Hartmann, G.K. MHz ATS-6 Radio Beacon". Submitted for publics- Leo , R.L

Validation of the Calypso Surface Beacon Transponder.

PubMed

Belanger, Maxwell; Saleh, Ziad; Volpe, Tom; Margiasso, Rich; Li, Xiang; Chan, Maria; Zhu, Xiaofeng; Tang, Xiaoli

2016-07-08

Calypso L-shaped Surface Beacon transponder has recently become available for clinical applications. We herein conduct studies to validate the Surface Beacon transponder in terms of stability, reproducibility, orientation sensitivity, cycle rate dependence, and respiratory waveform tracking accuracy. The Surface Beacon was placed on a Quasar respiratory phantom and positioned at the isocenter with its two arms aligned with the lasers. Breathing waveforms were simulated, and the motion of the transponder was tracked. Stability and drift analysis: sinusoidal waveforms (200 cycles) were produced, and the amplitudes of phases 0% (inhale) and 50% (exhale) were recorded at each breathing cycle. The mean and standard deviation (SD) of the amplitudes were calculated. Linear least-squares fitting was performed to access the possible amplitude drift over the breathing cycles. Reproducibility: similar setting to stability and drift analysis, and the phantom generated 100 cycles of the sinusoidal waveform per run. The Calypso system's was re-setup for each run. Recorded amplitude and SD of 0% and 50% phase were compared between runs to assess contribution of Calypso electromagnetic array setup variation. Beacon orientation sensitivity: the Calypso tracks sinusoidal phantom motion with a defined angular offset of the beacon to assess its effect on SD and peak-to-peak amplitude. Rate dependence: sinusoidal motion was generated at cycle rates of 1 Hz, .33 Hz, and .2 Hz. Peak-to-peak displacement and SDs were assessed. Respiratory waveform tracking accuracy: the phantom reproduced recorded breathing cycles (by volunteers and patients) were tracked by the Calypso system. Deviation in tracking position from produced waveform was used to calculate SD throughout entire breathing cycle. Stability and drift analysis: Mean amplitude ± SD of phase 0% or 50% were 20.01 ± 0.04 mm and -19.65 ± 0.08 mm, respectively. No clinically significant drift was detected with drift measured as 5.1 × 10-5 mm/s at phase 0% and -6.0 × 10-5 mm/s at phase 50%. Reproducibility: The SD of the setup was 0.06 mm and 0.02 mm for phases 0% and 50%, respectively. The combined SDs, including both setup and intrarun error of all runs at phases 0% and 50%, were 0.07mm and 0.11 mm, respectively. Beacon orientation: SD ranged from 0.032mm to 0.039 mm at phase 0% and from 0.084 mm to 0.096 mm at phase 50%. The SD was found not to vary linearly with Beacon angle in the range of 0° and 15°. A positive systematic error was observed with amplitude 0.07 mm/degree at phase 0% and 0.05 mm/degree at phase 50%. Rate dependence: SD and displacement amplitudes did not vary significantly between 0.2 Hz and 0.33 Hz. At 1 Hz, both 0% and 50% amplitude measurements shifted up appreciably, by 0.72 mm and 0.78mm, respectively. As compared with the 0.33 Hz data, SD at phase 0% was 1.6 times higher and 5.4 times higher at phase 50%. Respiratory waveform tracking accuracy: SD of 0.233 mm with approximately normal distribution in over 134 min of tracking (201468 data points). The Surface Beacon transponder appears to be stable, accurate, and reproducible. Submillimeter resolution is achieved throughout breathing and sinusoidal waveforms. © 2016 The Authors

Validation of the Calypso Surface Beacon Transponder

PubMed Central

Saleh, Ziad; Volpe, Tom; Margiasso, Rich; Li, Xiang; Chan, Maria; Zhu, Xiaofeng; Tang, Xiaoli

2016-01-01

Calypso L‐shaped Surface Beacon transponder has recently become available for clinical applications. We herein conduct studies to validate the Surface Beacon transponder in terms of stability, reproducibility, orientation sensitivity, cycle rate dependence, and respiratory waveform tracking accuracy. The Surface Beacon was placed on a Quasar respiratory phantom and positioned at the isocenter with its two arms aligned with the lasers. Breathing waveforms were simulated, and the motion of the transponder was tracked. Stability and drift analysis: sinusoidal waveforms (200 cycles) were produced, and the amplitudes of phases 0% (inhale) and 50% (exhale) were recorded at each breathing cycle. The mean and standard deviation (SD) of the amplitudes were calculated. Linear least‐squares fitting was performed to access the possible amplitude drift over the breathing cycles. Reproducibility: similar setting to stability and drift analysis, and the phantom generated 100 cycles of the sinusoidal waveform per run. The Calypso system's was re‐setup for each run. Recorded amplitude and SD of 0% and 50% phase were compared between runs to assess contribution of Calypso electromagnetic array setup variation. Beacon orientation sensitivity: the Calypso tracks sinusoidal phantom motion with a defined angular offset of the beacon to assess its effect on SD and peak‐to‐peak amplitude. Rate dependence: sinusoidal motion was generated at cycle rates of 1 Hz, .33 Hz, and .2 Hz. Peak‐to‐peak displacement and SDs were assessed. Respiratory waveform tracking accuracy: the phantom reproduced recorded breathing cycles (by volunteers and patients) were tracked by the Calypso system. Deviation in tracking position from produced waveform was used to calculate SD throughout entire breathing cycle. Stability and drift analysis: Mean amplitude ± SD of phase 0% or 50% were 20.01±0.04 mm and ‐19.65±0.08 mm, respectively. No clinically significant drift was detected with drift measured as 5.1×10‐5 mm/s at phase 0% and ‐6.0×10‐5 mm/s at phase 50%. Reproducibility: The SD of the setup was 0.06 mm and 0.02 mm for phases 0% and 50%, respectively. The combined SDs, including both setup and intrarun error of all runs at phases 0% and 50%, were 0.07 mm and 0.11 mm, respectively. Beacon orientation: SD ranged from 0.032 mm to 0.039 mm at phase 0% and from 0.084 mm to 0.096 mm at phase 50%. The SD was found not to vary linearly with Beacon angle in the range of 0° and 15°. A positive systematic error was observed with amplitude 0.07 mm/degree at phase 0% and 0.05 mm/degree at phase 50%. Rate dependence: SD and displacement amplitudes did not vary significantly between 0.2 Hz and 0.33 Hz. At 1 Hz, both 0% and 50% amplitude measurements shifted up appreciably, by 0.72 mm and 0.78 mm, respectively. As compared with the 0.33 Hz data, SD at phase 0% was 1.6 times higher and 5.4 times higher at phase 50%. Respiratory waveform tracking accuracy: SD of 0.233 mm with approximately normal distribution in over 134 min of tracking (201468 data points). The Surface Beacon transponder appears to be stable, accurate, and reproducible. Submillimeter resolution is achieved throughout breathing and sinusoidal waveforms. PACS number(s): 87.50.ct, 87.50.st, 87.50.ux, 87.50.wp, 87.50.yt PMID:27455489

Toward observationally constrained high space and time resolution CO2 urban emission inventories

NASA Astrophysics Data System (ADS)

Maness, H.; Teige, V. E.; Wooldridge, P. J.; Weichsel, K.; Holstius, D.; Hooker, A.; Fung, I. Y.; Cohen, R. C.

2013-12-01

The spatial patterns of greenhouse gas (GHG) emission and sequestration are currently studied primarily by sensor networks and modeling tools that were designed for global and continental scale investigations of sources and sinks. In urban contexts, by design, there has been very limited investment in observing infrastructure, making it difficult to demonstrate that we have an accurate understanding of the mechanism of emissions or the ability to track processes causing changes in those emissions. Over the last few years, our team has built a new high-resolution observing instrument to address urban CO2 emissions, the BErkeley Atmospheric CO2 Observing Network (BEACON). The 20-node network is constructed on a roughly 2 km grid, permitting direct characterization of the internal structure of emissions within the San Francisco East Bay. Here we present a first assessment of BEACON's promise for evaluating the effectiveness of current and upcoming local emissions policy. Within the next several years, a variety of locally important changes are anticipated--including widespread electrification of the motor vehicle fleet and implementation of a new power standard for ships at the port of Oakland. We describe BEACON's expected performance for detecting these changes, based on results from regional forward modeling driven by a suite of projected inventories. We will further describe the network's current change detection capabilities by focusing on known high temporal frequency changes that have already occurred; examples include a week of significant freeway traffic congestion following the temporary shutdown of the local commuter rail (the Bay Area Rapid Transit system).

BEACON: A Summary Framework to Overcome Potential Reimbursement Hurdles.

PubMed

Dunlop, William C N; Mullins, C Daniel; Pirk, Olaf; Goeree, Ron; Postma, Maarten J; Enstone, Ashley; Heron, Louise

2016-10-01

To provide a framework for addressing payers' criteria during the development of pharmaceuticals. A conceptual framework was presented to an international health economic expert panel for discussion. A structured literature search (from 2010 to May 2015), using the following databases in Ovid: Medline(®) and Medline(®) In-Process (PubMed), Embase (Ovid), EconLit (EBSCOhost) and the National Health Service Economic Evaluation Database (NHS EED), and a 'grey literature' search, were conducted to identify existing criteria from the payer perspective. The criteria assessed by existing frameworks and guidelines were collated; the most commonly reported criteria were considered for inclusion in the framework. A mnemonic was conceived as a memory aide to summarise these criteria. Overall, 41 publications were identified as potentially relevant to the objective. Following further screening, 26 were excluded upon full-text review on the basis of no framework presented (n = 13), redundancy (n = 11) or abstract only (n = 2). Frameworks that captured criteria developed for or utilised by the pharmaceutical industry (n = 5) and reimbursement guidance (n = 10) were reviewed. The most commonly identified criteria-unmet need/patient burden, safety, efficacy, quality-of-life outcomes, environment, evidence quality, budget impact and comparator-were incorporated into the summary framework. For ease of communication, the following mnemonic was developed: BEACON (Burden/target population, Environment, Affordability/value, Comparator, Outcomes, Number of studies/quality of evidence). The BEACON framework aims to capture the 'essence' of payer requirements by addressing the most commonly described criteria requested by payers regarding the introduction of a new pharmaceutical.

Epilithic lichens in the Beacon sandstone formation, Victoria Land, Antarctica

NASA Technical Reports Server (NTRS)

Hale, M. E.; Friedmann, E. I. (Principal Investigator)

1987-01-01

The epilithic lichen flora on the Beacon sandstone formation in Victoria Land consists of seven species: Acarospora gwynnii Dodge & Rudolph, Buellia grisea Dodge & Baker, B. pallida Dodge & Baker, Carbonea capsulata (Dodge & Baker) Hale comb. nov., Lecanora fuscobrunnea Dodge & Baker, Lecidea cancriformis Dodge & Baker, and L. siplei Dodge & Baker. The typification of the species is given along with descriptions and distribution in Antarctica.

Characterization of Atmospheric Turbulence Effects Over 149 km Propagation Path using Multi-Wavelength Laser Beacons

DTIC Science & Technology

2010-09-01

received beams (Fig. 2). Narrow bandpass filters were used to dedicate each subaperture to a specific wave from a single beacon. In this paper we...r , (6) where 1 1 ( )Mn n mmI M I − = = ∑ r is the aperture-average intensity for the nth frame. The index S in Eq. (6) denotes averaging over

Flight Software Implementation of the Beacon Monitor Expreiment On the NASA New Millennium Deep Space 1 (DS-1) Mission

NASA Technical Reports Server (NTRS)

Foster, R.; Schlutsmeyer, A.

1997-01-01

A new technology that can lower the cost of mission operations on future spacecraft will be tested on the NASA New Millennium Deep Space 1 (DS-1) Mission. This technology, the Beacon Monitor Experiment (BMOX), can be used to reduce the Deep Space Network (DSN) tracking time and its associated costs on future missions.

Early Detection of Breast Cancer Using Molecular Beacons

DTIC Science & Technology

2008-01-01

a molecular beacon (MB)-based approach for direct examination of gene expression in viable and fixed cells (2, 3). This objective of proposed study ...can be distinguished from normal cells (dark) (Figure 1) (2, 3, 8). Recently, a class of new fluorescent emitting nanoparticles, semiconductor ...morphological classification. This method may offer a simple and fast procedure to detect biomarker gene expression in clinical samples. Our study results

Modeling Self-Referencing Interferometers with Extended Beacons and Strong Turbulence

DTIC Science & Technology

2011-09-01

identified then typically compensated. These results not only serve to address problems when using adaptive optics to correct for strong turbulence ...compensat- ing for distortions due to atmospheric turbulence with adaptive optics (AO) [70, 84]. AO typically compensates for atmospheric distortions... used in Chapter VII to discuss how strong atmospheric turbulence and extended beacons affect the performance of an SRI. Additionally, it enumerates the

Data Governance and Data Sharing Agreements for Community-Wide Health Information Exchange: Lessons from the Beacon Communities

PubMed Central

Allen, Claudia; Des Jardins, Terrisca R.; Heider, Arvela; Lyman, Kristin A.; McWilliams, Lee; Rein, Alison L.; Schachter, Abigail A.; Singh, Ranjit; Sorondo, Barbara; Topper, Joan; Turske, Scott A.

2014-01-01

Purpose: Unprecedented efforts are underway across the United States to electronically capture and exchange health information to improve health care and population health, and reduce costs. This increased collection and sharing of electronic patient data raises several governance issues, including privacy, security, liability, and market competition. Those engaged in such efforts have had to develop data sharing agreements (DSAs) among entities involved in information exchange, many of whom are “nontraditional” health care entities and/or new partners. This paper shares lessons learned based on the experiences of six federally funded communities participating in the Beacon Community Cooperative Agreement Program, and offers guidance for navigating data governance issues and developing DSAs to facilitate community-wide health information exchange. Innovation: While all entities involved in electronic data sharing must address governance issues and create DSAs accordingly, until recently little formal guidance existed for doing so – particularly for community-based initiatives. Despite this lack of guidance, together the Beacon Communities’ experiences highlight promising strategies for navigating complex governance issues, which may be useful to other entities or communities initiating information exchange efforts to support delivery system transformation. Credibility: For the past three years, AcademyHealth has provided technical assistance to most of the 17 Beacon Communities, 6 of whom contributed to this collaborative writing effort. Though these communities varied widely in terms of their demographics, resources, and Beacon-driven priorities, common themes emerged as they described their approaches to data governance and DSA development. Conclusions: The 6 Beacon Communities confirmed that DSAs are necessary to satisfy legal and market-based concerns, and they identified several specific issues, many of which have been noted by others involved in network data sharing initiatives. More importantly, these communities identified several promising approaches to timely and effective DSA development, including: stakeholder engagement; identification and effective communication of value; adoption of a parsimonious approach; attention to market-based concerns; flexibility in adapting and expanding existing agreements and partnerships; and anticipation of required time and investment. PMID:25848589

Selective amyloid β oligomer assay based on abasic site-containing molecular beacon and enzyme-free amplification.

PubMed

Zhu, Linling; Zhang, Junying; Wang, Fengyang; Wang, Ya; Lu, Linlin; Feng, Chongchong; Xu, Zhiai; Zhang, Wen

2016-04-15

Amyloid-beta (Aβ) oligomers are highly toxic species in the process of Aβ aggregation and are regarded as potent therapeutic targets and diagnostic markers for Alzheimer's disease (AD). Herein, a label-free molecular beacon (MB) system integrated with enzyme-free amplification strategy was developed for simple and highly selective assay of Aβ oligomers. The MB system was constructed with abasic site (AP site)-containing stem-loop DNA and a fluorescent ligand 2-amino-5,6,7-trimethyl-1,8-naphyridine (ATMND), of which the fluorescence was quenched upon binding to the AP site in DNA stem. Enzyme-free amplification was realized by target-triggered continuous opening of two delicately designed MBs (MB1 and MB2). Target DNA hybridization with MB1 and then MB2 resulted in the release of two ATMND molecules in one binding event. Subsequent target recycling could greatly amplify the detection sensitivity due to the greatly enhanced turn-on emission of ATMND fluorescence. Combining with Aβ oligomers aptamers, the strategy was applied to analyze Aβ oligomers and the results showed that it could quantify Aβ oligomers with high selectivity and monitor the Aβ aggregation process. This novel method may be conducive to improve the diagnosis and pathogenic study of Alzheimer's disease. Copyright © 2015 Elsevier B.V. All rights reserved.

OPEX propagation measurements and studies

NASA Technical Reports Server (NTRS)

Arbesser-Rastburg, Bertram

1990-01-01

With the launch of the telecommunications Olympus satellite a new area began for the Olympus Propagation Experiments (OPEX) group. The years of preparations are now paying off - the experiments are underway and the co-operative effort is now turning its attention to the processing and analysis of data and to the interpretation of results. The aim here is to give a short review of the accomplishments made since NAPEX 13 and the work planned for the future. When ESA's Olympus was launched in summer of 1989 it carried a payload producing three unmodulated beacons at 12.5, 19.8, and 29.7 GHz. The main purpose of these beacons is to enable scientists to carry out long term slant path propagation experiments at these frequencies. The OPEX group, which was set up under ESA auspices in 1980, had been preparing for this event very carefully. The specifications for the equipment to be used and the elaboration of standard procedures for data processing and analysis have been worked out jointly. Today the OPEX community includes approximately 30 groups of experimenters. Immediately after achieving platform stability at the orbital location at 341 degrees east, ESA performed the In-Orbit Tests. Most measurements were carried out in Belgium using terminals specially developed for this purpose. A summary of the test results is given.

Baseline characteristics in the Bardoxolone methyl EvAluation in patients with Chronic kidney disease and type 2 diabetes mellitus: the Occurrence of renal eveNts (BEACON) trial.

PubMed

Lambers Heerspink, Hiddo J; Chertow, Glenn M; Akizawa, Tadao; Audhya, Paul; Bakris, George L; Goldsberry, Angie; Krauth, Melissa; Linde, Peter; McMurray, John J; Meyer, Colin J; Parving, Hans-Henrik; Remuzzi, Giuseppe; Christ-Schmidt, Heidi; Toto, Robert D; Vaziri, Nosratola D; Wanner, Christoph; Wittes, Janet; Wrolstad, Danielle; de Zeeuw, Dick

2013-11-01

Type 2 diabetes mellitus (T2DM) is the most important contributing cause of end-stage renal disease (ESRD) worldwide. Bardoxolone methyl, a nuclear factor-erythroid-2-related factor 2 activator, augments estimated glomerular filtration. The Bardoxolone methyl EvAluation in patients with Chronic kidney disease and type 2 diabetes mellitus: the Occurrence of renal eveNts (BEACON) trial was designed to establish whether bardoxolone methyl slows or prevents progression to ESRD. Herein, we describe baseline characteristics of the BEACON population. BEACON is a randomized double-blind placebo-controlled clinical trial in 2185 patients with T2DM and chronic kidney disease stage 4 (eGFR between 15 and 30 mL/min/1.73 m(2)) designed to test the hypothesis that bardoxolone methyl added to guideline-recommended treatment including inhibitors of the renin-angiotensin-aldosterone system slows or prevents progression to ESRD or cardiovascular death compared with placebo. Baseline characteristics (mean or percentage) of the population include age 68.5 years, female 43%, Caucasian 78%, eGFR 22.5 mL/min/1.73 m(2) and systolic/diastolic blood pressure 140/70 mmHg. The median urinary albumin:creatinine ratio was 320 mg/g and the frequency of micro- and macroalbuminuria was 30 and 51%, respectively. Anemia, abnormalities in markers of bone metabolism and elevations in cardiovascular biomarkers were frequently observed. A history of cardiovascular disease was present in 56%, neuropathy in 47% and retinopathy in 41% of patients. The BEACON trial enrolled a population heretofore unstudied in an international randomized controlled trial. Enrolled patients suffered with numerous co-morbid conditions and exhibited multiple laboratory abnormalities, highlighting the critical need for new therapies to optimize management of these conditions.

Aftermath of bustamante attack on genomic beacon service.

PubMed

Aziz, Md Momin Al; Ghasemi, Reza; Waliullah, Md; Mohammed, Noman

2017-07-26

With the enormous need for federated eco-system for holding global genomic and clinical data, Global Alliance for Genomic and Health (GA4GH) has created an international website called beacon service which allows a researcher to find out whether a specific dataset can be utilized to his or her research beforehand. This simple webservice is quite useful as it allows queries like whether a certain position of a target chromosome has a specific nucleotide. However, the increased integration of individuals genomic data into clinical practice and research raised serious privacy concern. Though the answer of such queries are yes or no in Bacon network, it results in serious privacy implication as demonstrated in a recent work from Shringarpure and Bustamante. In their attack model, the authors demonstrated that with a limited number of queries, presence of an individual in any dataset can be determined. We propose two lightweight algorithms (based on randomized response) which captures the efficacy while preserving the privacy of the participants in a genomic beacon service. We also elaborate the strength and weakness of the attack by explaining some of their statistical and mathematical models using real world genomic database. We extend their experimental simulations for different adversarial assumptions and parameters. We experimentally evaluated the solutions on the original attack model with different parameters for better understanding of the privacy and utility tradeoffs provided by these two methods. Also, the statistical analysis further elaborates the different aspects of the prior attack which leads to a better risk management for the participants in a beacon service. The differentially private and lightweight solutions discussed here will make the attack much difficult to succeed while maintaining the fundamental motivation of beacon database network.

United States Navy - Canadian forces solid state flight data recorder/crash position locator experiment on the B-720 controlled impact demonstration

NASA Technical Reports Server (NTRS)

Watters, D. M.

1986-01-01

The operation of a radio beacon position locator during and after the remotely controlled transport aircraft is discussed. The radio beacon transmission was actuated and was picked up by the Navy P-3A chase aircraft for a short time, after which reception was lost. The pilot reported that he received a signal on both 121.5 MHz and 243 MHz for a period of approximately 5 seconds. Five minutes after the crash a portable direction finding unit located on the roof of the NASA Dryden Flight Research Facility, 4 miles distant from the crash, was unable to pick up the beacon transmission. The fire crews started fighting the fires approximately 90 seconds after the time of impact. Navy personnel access to the crash site was allowed on the morning of December 2, 1984. Radio beacon locator was found resting top side up, 15 feet forward and 13 feet perpendicular from the tray location the starboard side of the aircraft. An immediate inspection indicated the airfoil suffered moderate fire damage with paint peeling but not intumescing. The visual marker strobe lamp housings were intact but extensively burned such that it was impossible to see if the lamps had survived. The airfoil suffered minor structural damage, with assorted dents, etc. The extended plunger on the ARU-21 release unit indicated that the pyrotechnic deployment system operated. The radio beacon base (tray) suffered some heat and fire damage, and was charred and blackened by smoke. The frangible switch in the nose survived and the switch in the belly was recovered and found to have actuated. It is assumed that this switch fired the ARU-21 squib. There were no other release switches installed in the normally open system in the aircraft.

Increased dose near the skin due to electromagnetic surface beacon transponder.

PubMed

Ahn, Kang-Hyun; Manger, Ryan; Halpern, Howard J; Aydogan, Bulent

2015-05-08

The purpose of this study was to evaluate the increased dose near the skin from an electromagnetic surface beacon transponder, which is used for localization and tracking organ motion. The bolus effect due to the copper coil surface beacon was evaluated with radiographic film measurements and Monte Carlo simulations. Various beam incidence angles were evaluated for both 6 MV and 18 MV experimentally. We performed simulations using a general-purpose Monte Carlo code MCNPX (Monte Carlo N-Particle) to supplement the experimental data. We modeled the surface beacon geometry using the actual mass of the glass vial and copper coil placed in its L-shaped polyethylene terephthalate tubing casing. Film dosimetry measured factors of 2.2 and 3.0 enhancement in the surface dose for normally incident 6 MV and 18 MV beams, respectively. Although surface dose further increased with incidence angle, the relative contribution from the bolus effect was reduced at the oblique incidence. The enhancement factors were 1.5 and 1.8 for 6 MV and 18 MV, respectively, at an incidence angle of 60°. Monte Carlo simulation confirmed the experimental results and indicated that the epidermal skin dose can reach approximately 50% of the dose at dmax at normal incidence. The overall effect could be acceptable considering the skin dose enhancement is confined to a small area (~ 1 cm2), and can be further reduced by using an opposite beam technique. Further clinical studies are justified in order to study the dosimetric benefit versus possible cosmetic effects of the surface beacon. One such clinical situation would be intact breast radiation therapy, especially large-breasted women.

Synchronous identification of friendly targets

DOEpatents

Telle, John M.; Roger, Stutz A.

1998-01-01

A synchronous communication targeting system for use in battle. The present invention includes a transceiver having a stabilizing oscillator, a synchronous amplifier and an omnidirectional receiver, all in electrical communication with each other. A remotely located beacon is attached to a blackbody radiation source and has an amplitude modulator in electrical communication with a optical source. The beacon's amplitude modulator is set so that the optical source transmits radiation frequency at approximately the same or lower amplitude than that of the blackbody radiation source to which the beacon is attached. The receiver from the transceiver is adapted to receive frequencies approximately at or below blackbody radiation signals and sends such signals to the synchronous amplifier. The synchronous amplifier then rectifies and amplifies those signals which correspond to the predetermined frequency to therefore identify whether the blackbody radiation source is friendly or not.

Method and apparatus for a multibeam beacon laser assembly for optical communications

NASA Technical Reports Server (NTRS)

Biswas, Abhijit (Inventor); Sanji, Babak (Inventor); Wright, Malcolm W. (Inventor); Page, Norman Alan (Inventor)

2005-01-01

An optical beacon is comprised of a telescope having a primary focal plane or Coud? focal plane, a plurality of fiber coupled laser sources for generating a plurality of beams, a collimator for collimating the plurality of beams, and optics for combining and focusing the plurality of collimated beams onto the primary or Coud? focal plane of the telescope. The telescope propagates the optical beacon, which is arranged into a ring of incoherent plurality of collimated beams. The apparatus further comprises fiber splitters coupled to each laser source to provide at least eight beams from at least four laser sources. The optics comprises a prism assembly, a combiner lens, a focusing lens and a field lens for focusing the plurality of collimated beams onto the primary focal plane or Coud? focal plane of the telescope.

Comparison of OLYMPUS beacon and radiometric attenuation measurements at Blacksburg, Virginia

NASA Technical Reports Server (NTRS)

Snider, J. B.; Jacobson, M. D.; Beeler, R. H.; Hazen, D. A.

1991-01-01

Measurements of attenuation of the 20 and 30 GHz beacons onboard the OLYMPUS satellite are compared to simultaneous observations of atmospheric attenuation by a multichannel microwave radiometer along the same path. Departures from high correlation between the two measurements are believed to be related to differences in antenna beamwidths. Mean equivalent zenith attenuations derived from the slant path data are compared to zenith observations made at previous locations.

Secure Overlay Services (SOS)

DTIC Science & Technology

2004-08-01

special node in the SOS architecture that is easily reached, called the beacon. 3. The beacon forwards the packet to a “secret” node, called the secret servlet...whose identity is known to only a small subset of participants in the SOS architecture. 6 4. The secret servlet forwards the packet to...address is the secret servlet. In the following discussion, we motivate why the SOS architecture requires the series of steps described above

Crash Position Indicator/Crash Survivable Flight Data Recorder Ejectable versus Nonejectable

DTIC Science & Technology

1983-07-27

for environmental protection. The radio beacon transmitter, antenna, and manual shutoff switch are molded into the airfoil foam. The battery and CSFDR...FWD BATERY LRADIO) BEACON ANTENNA TRANSMITTER SOLID-STATE FLIGHT INCIDENT RECORDER Figure 2 DEPLOYABLE FLIGHT INCIDENT RECORDER/CRASH POSITION LOCATOR...mechanical and thermal environment than do ejectable systems that depart the aircraft and clear the crash and fire. As a result of the more stringent

78 FR 73553 - Prospective Grant of Exclusive License: Development of Cripto-1 Point of Care (POC) Tests and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-06

...This is notice, in accordance with 35 U.S.C. 209 and 37 CFR Part 404, that the National Institutes of Health, Department of Health and Human Services, is contemplating the grant of an exclusive patent license to practice the inventions embodied in the following U.S. Patents and Patent Applications to Beacon Biomedical LLC (``Beacon'') located in Scottsdale, AZ, USA.

Integrated Formulation of Beacon-Based Exception Analysis for Multimissions

NASA Technical Reports Server (NTRS)

Mackey, Ryan; James, Mark; Park, Han; Zak, Mickail

2003-01-01

Further work on beacon-based exception analysis for multimissions (BEAM), a method of real-time, automated diagnosis of a complex electromechanical systems, has greatly expanded its capability and suitability of application. This expanded formulation, which fully integrates physical models and symbolic analysis, is described. The new formulation of BEAM expands upon previous advanced techniques for analysis of signal data, utilizing mathematical modeling of the system physics, and expert-system reasoning,

a Search for New Physics with the Beacon Mission

NASA Astrophysics Data System (ADS)

Turyshev, Slava G.; Shao, Michael; Girerd, André; Lane, Benjamin

The primary objective of the Beyond Einstein Advanced Coherent Optical Network (BEACON) mission is a search for new physics beyond general relativity by measuring the curvature of relativistic space-time around the Earth. This curvature is characterized by the Eddington parameter γ — the most fundamental relativistic gravity parameter and a direct measure for the presence of new physical interactions. BEACON will achieve an accuracy of 1 × 10-9 in measuring the parameter γ, thereby going a factor of 30,000 beyond the present best result involving the Cassini spacecraft. Secondary mission objectives include: (i) a direct measurement of the "frame-dragging" and geodetic precessions in the Earth's rotational gravitomagnetic field, to 0.05% and 0.03% accuracy respectively, (ii) the first measurement of gravity's nonlinear effects on light and the corresponding second order spatial metric's effects to 0.01% accuracy. BEACON will lead to robust advances in tests of fundamental physics — this mission could discover a violation or extension of general relativity and/or reveal the presence of an additional long range interaction in physics. It will provide crucial information to separate modern scalar-tensor theories of gravity from general relativity, probe possible ways for gravity quantization, and test modern theories of cosmological evolution.

Molecular beacon probes-base multiplex NASBA Real-time for detection of HIV-1 and HCV.

PubMed

Mohammadi-Yeganeh, S; Paryan, M; Mirab Samiee, S; Kia, V; Rezvan, H

2012-06-01

Developed in 1991, nucleic acid sequence-based amplification (NASBA) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than PCR, and it can also be more sensitive. This paper describes the development of a molecular beacon-based multiplex NASBA assay for simultaneous detection of HIV-1 and HCV in plasma samples. A well-conserved region in the HIV-1 pol gene and 5'-NCR of HCV genome were used for primers and molecular beacon design. The performance features of HCV/HIV-1 multiplex NASBA assay including analytical sensitivity and specificity, clinical sensitivity and clinical specificity were evaluated. The analysis of scalar concentrations of the samples indicated that the limit of quantification of the assay was <1000 copies/ml for HIV-1 and <500 copies/ml for HCV with 95% confidence interval. Multiplex NASBA assay showed a 98% sensitivity and 100% specificity. The analytical specificity study with BLAST software demonstrated that the primers do not attach to any other sequences except for that of HIV-1 or HCV. The primers and molecular beacon probes detected all HCV genotypes and all major variants of HIV-1. This method may represent a relatively inexpensive isothermal method for detection of HIV-1/HCV co-infection in monitoring of patients.

Estimation of distributed Fermat-point location for wireless sensor networking.

PubMed

Huang, Po-Hsian; Chen, Jiann-Liang; Larosa, Yanuarius Teofilus; Chiang, Tsui-Lien

2011-01-01

This work presents a localization scheme for use in wireless sensor networks (WSNs) that is based on a proposed connectivity-based RF localization strategy called the distributed Fermat-point location estimation algorithm (DFPLE). DFPLE applies triangle area of location estimation formed by intersections of three neighboring beacon nodes. The Fermat point is determined as the shortest path from three vertices of the triangle. The area of estimated location then refined using Fermat point to achieve minimum error in estimating sensor nodes location. DFPLE solves problems of large errors and poor performance encountered by localization schemes that are based on a bounding box algorithm. Performance analysis of a 200-node development environment reveals that, when the number of sensor nodes is below 150, the mean error decreases rapidly as the node density increases, and when the number of sensor nodes exceeds 170, the mean error remains below 1% as the node density increases. Second, when the number of beacon nodes is less than 60, normal nodes lack sufficient beacon nodes to enable their locations to be estimated. However, the mean error changes slightly as the number of beacon nodes increases above 60. Simulation results revealed that the proposed algorithm for estimating sensor positions is more accurate than existing algorithms, and improves upon conventional bounding box strategies.

Fundamental limits on isoplanatic correction with multiconjugate adaptive optics

NASA Astrophysics Data System (ADS)

Lloyd-Hart, Michael; Milton, N. Mark

2003-10-01

We investigate the performance of a general multiconjugate adaptive optics (MCAO) system in which signals from multiple reference beacons are used to drive several deformable mirrors in the optical beam train. Taking an analytic approach that yields a detailed view of the effects of low-order aberration modes defined over the metapupil, we show that in the geometrical optics approximation, N deformable mirrors conjugated to different ranges can be driven to correct these modes through order N with unlimited isoplanatic angle, regardless of the distribution of turbulence along the line of sight. We find, however, that the optimal deformable mirror shapes are functions of target range, so the best compensation for starlight is in general not the correction that minimizes the wave-front aberration in a laser guide beacon. This introduces focal anisoplanatism in the wave-front measurements that can be overcome only through the use of beacons at several ranges. We derive expressions for the number of beacons required to sense the aberration to arbitrary order and establish necessary and sufficient conditions on their geometry for both natural and laser guide stars. Finally, we derive an expression for the residual uncompensated error by mode as a function of field angle, target range, and MCAO system geometry.

Micro-machined resonator oscillator

DOEpatents

Koehler, D.R.; Sniegowski, J.J.; Bivens, H.M.; Wessendorf, K.O.

1994-08-16

A micro-miniature resonator-oscillator is disclosed. Due to the miniaturization of the resonator-oscillator, oscillation frequencies of one MHz and higher are utilized. A thickness-mode quartz resonator housed in a micro-machined silicon package and operated as a telemetered sensor beacon'' that is, a digital, self-powered, remote, parameter measuring-transmitter in the FM-band. The resonator design uses trapped energy principles and temperature dependence methodology through crystal orientation control, with operation in the 20--100 MHz range. High volume batch-processing manufacturing is utilized, with package and resonator assembly at the wafer level. Unique design features include squeeze-film damping for robust vibration and shock performance, capacitive coupling through micro-machined diaphragms allowing resonator excitation at the package exterior, circuit integration and extremely small (0.1 in. square) dimensioning. A family of micro-miniature sensor beacons is also disclosed with widespread applications as bio-medical sensors, vehicle status monitors and high-volume animal identification and health sensors. The sensor family allows measurement of temperatures, chemicals, acceleration and pressure. A microphone and clock realization is also available. 21 figs.

Imaging the Directed Transport of Single Engineered RNA Transcripts in Real-Time Using Ratiometric Bimolecular Beacons

PubMed Central

Zhang, Xuemei; Zajac, Allison L.; Huang, Lingyan; Behlke, Mark A.; Tsourkas, Andrew

2014-01-01

The relationship between RNA expression and cell function can often be difficult to decipher due to the presence of both temporal and sub-cellular processing of RNA. These intricacies of RNA regulation can often be overlooked when only acquiring global measurements of RNA expression. This has led to development of several tools that allow for the real-time imaging of individual engineered RNA transcripts in living cells. Here, we describe a new technique that utilizes an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), to image RNA transcripts that were engineered to contain 96-tandem repeats of the RBMB target sequence in the 3′-untranslated region. Binding of RBMBs to the target RNA resulted in discrete bright fluorescent spots, representing individual transcripts, that could be imaged in real-time. Since RBMBs are a synthetic probe, the use of photostable, bright, and red-shifted fluorophores led to a high signal-to-background. RNA motion was readily characterized by both mean squared displacement and moment scaling spectrum analyses. These analyses revealed clear examples of directed, Brownian, and subdiffusive movements. PMID:24454933

Measuring weather for aviation safety in the 1980's

NASA Technical Reports Server (NTRS)

Wedan, R. W.

1980-01-01

Requirements for an improved aviation weather system are defined and specifically include the need for (1) weather observations at all airports with instrument approaches, (2) more accurate and timely radar detection of weather elements hazardous to aviation, and (3) better methods of timely distribution of both pilot reports and ground weather data. The development of the discrete address beacon system data link, Doppler weather radar network, and various information processing techniques are described.

Design of a K/Q-Band Beacon Receiver for the Alphasat TDP#5 Experiment

NASA Technical Reports Server (NTRS)

Morse, Jacquelynne R.

2014-01-01

This paper describes the design and performance of a coherent KQ-band (2040 GHz) beacon receiver developed at NASA Glenn Research Center (GRC) that will be installed at the Politecnico di Milano (POLIMI) for use in the Alphasat Technology Demonstration Payload 5 (TDP5) beacon experiment. The goal of this experiment is to characterize rain fade attenuation at 40 GHz to improve the performance of existing statistical rain attenuation models in the Q-band. The ground terminal developed by NASA GRC utilizes an FFT-based frequency estimation receiver capable of characterizing total path attenuation effects due to gaseous absorption, clouds, rain, and scintillation. The receiver system has been characterized in the lab and demonstrates a system dynamic range performance of better than 58 dB at 1 Hz and better than 48 dB at 10 Hz rates.

Design of a K/Q-Band Beacon Receiver for the Alphasat Technology Demonstration Payload (TDP) #5 Experiment

NASA Technical Reports Server (NTRS)

Morse, Jacquelynne R.

2014-01-01

This paper describes the design and performance of a coherent KQ-band (2040 GHz) beacon receiver developed at NASA Glenn Research Center (GRC) that will be installed at the Politecnico di Milano (POLIMI) for use in the Alphasat Technology Demonstration Payload 5 (TDP5) beacon experiment. The goal of this experiment is to characterize rain fade attenuation at 40 GHz to improve the performance of existing statistical rain attenuation models in the Q-band. The ground terminal developed by NASA GRC utilizes an FFT-based frequency estimation receiver capable of characterizing total path attenuation effects due to gaseous absorption, clouds, rain, and scintillation. The receiver system has been characterized in the lab and demonstrates a system dynamic range performance of better than 58 dB at 1 Hz and better than 48 dB at 10 Hz rates.

Switchable cucurbituril-bipyridine beacons.

PubMed

Sinha, Mantosh K; Reany, Ofer; Parvari, Galit; Karmakar, Ananta; Keinan, Ehud

2010-08-09

4-Aminobipyridine derivatives form strong inclusion complexes with cucurbit[6]uril, exhibiting remarkably large enhancements in fluorescence intensity and quantum yields. The remarkable complexation-induced pK(a) shift (DeltapK(a)=3.3) highlights the strong charge-dipole interaction upon binding. The reversible binding phenomenon can be used for the design of switchable beacons that can be incorporated into cascades of binding networks. This concept is demonstrated herein by three different applications: 1) a switchable fluorescent beacon for chemical sensing of transition metals and other ligands; 2) direct measurement of binding constants between cucurbit[6]uril and various nonfluorescent guest molecules; and 3) quantitative monitoring of biocatalytic reactions and determination of their kinetic parameters. The latter application is illustrated by the hydrolysis of an amide catalyzed by penicillin G acylase and by the elimination reaction of a beta-cabamoyloxy ketone catalyzed by aldolase antibody 38C2.

Design of a Combined Beacon Receiver and Digital Radiometer for 40 GHz Propagation Measurements at the Madrid Deep Space Communications Complex

NASA Technical Reports Server (NTRS)

Zemba, Michael J.; Nessel, James A.; Morabito, David D.

2017-01-01

NASA Glenn Research Center (GRC) and the Jet Propulsion Laboratory (JPL) have jointly developed an atmospheric propagation terminal to measure and characterize propagation phenomena at 40 GHz at the Madrid Deep Space Communications Complex (MDSCC) in Robledo de Chavela, Spain. The hybrid Q-band system combines a 40 GHz beacon receiver and digital radiometer into the same RF front-end and observes the 39.402 GHz beacon of the European Space Agencys Alphasat Aldo Paraboni TDP5 experiment. The goals of these measurements are to assist MDSCC mission operations as well as to contribute to the development and improvement of International Telecommunications Union (ITU) models for prediction of communications systems performance within the Q-band. Herein, we provide an overview of the system design, characterization, and plan of operations to commence at the MDSCC beginning in March 2017.

A molecular beacon based on DNA-templated silver nanoclusters for the highly sensitive and selective multiplexed detection of virulence genes.

PubMed

Han, Dan; Wei, Chunying

2018-05-01

In this work, we develop a fluorescent molecular beacon based on the DNA-templated silver nanoclusters (DNA-Ag NCs). The skillfully designed molecular beacon can be conveniently used for detection of diverse virulence genes as long as the corresponding recognition sequences are embedded. Importantly, the constructed detection system allows simultaneous detection of multiple nucleic acids, which is attributed to non-overlapping emission spectra of the as-synthesized silver nanoclusters. Based on the target-induced fluorescence enhancement, three infectious disease-related genes HIV, H1N1, and H5N1 are detected, and the corresponding detection limits are 3.53, 0.12 and 3.95nM, respectively. This design allows specific, versatile and simultaneous detection of diverse targets with easy operation and low cost. Copyright © 2017 Elsevier B.V. All rights reserved.

Design of a K/Q-band Beacon Receiver for the Alphasat TDP#5 Experiment

NASA Technical Reports Server (NTRS)

Nessel, James A.; Zemba, Michael J.; Morse, Jacquelynne R.

2014-01-01

This paper describes the design and performance of a coherent K/Q-band (20/40GHz) beacon receiver developed at NASA Glenn Research Center (GRC) that will be installed at the Politecnico di Milano (POLIMI) for use in the Alphasat Technology Demonstration Payload #5 (TDP#5) beacon experiment. The goal of this experiment is to characterize rain fade attenuation at 40GHz to improve the performance of existing statistical rain attenuation models in the Q-band. The ground terminal developed by NASA GRC utilizes an FFT-based frequency estimation receiver capable of characterizing total path attenuation effects due to gaseous absorption, clouds, rain, and scintillation. The receiver system has been characterized in the lab and demonstrates a system dynamic range performance of better than 58dB at 1Hz and better than 48dB at 10Hz rates.

Autonomous navigation using lunar beacons

NASA Technical Reports Server (NTRS)

Khatib, A. R.; Ellis, J.; French, J.; Null, G.; Yunck, T.; Wu, S.

1983-01-01

The concept of using lunar beacon signal transmission for on-board navigation for earth satellites and near-earth spacecraft is described. The system would require powerful transmitters on the earth-side of the moon's surface and black box receivers with antennae and microprocessors placed on board spacecraft for autonomous navigation. Spacecraft navigation requires three position and three velocity elements to establish location coordinates. Two beacons could be soft-landed on the lunar surface at the limits of allowable separation and each would transmit a wide-beam signal with cones reaching GEO heights and be strong enough to be received by small antennae in near-earth orbit. The black box processor would perform on-board computation with one-way Doppler/range data and dynamical models. Alternatively, GEO satellites such as the GPS or TDRSS spacecraft can be used with interferometric techniques to provide decimeter-level accuracy for aircraft navigation.

Mars approach navigation using Doppler and range measurements to surface beacons and orbiting spacecraft

NASA Technical Reports Server (NTRS)

Thurman, Sam W.; Estefan, Jeffrey A.

1991-01-01

Approximate analytical models are developed and used to construct an error covariance analysis for investigating the range of orbit determination accuracies which might be achieved for typical Mars approach trajectories. The sensitivity or orbit determination accuracy to beacon/orbiter position errors and to small spacecraft force modeling errors is also investigated. The results indicate that the orbit determination performance obtained from both Doppler and range data is a strong function of the inclination of the approach trajectory to the Martian equator, for surface beacons, and for orbiters, the inclination relative to the orbital plane. Large variations in performance were also observed for different approach velocity magnitudes; Doppler data in particular were found to perform poorly in determining the downtrack (along the direction of flight) component of spacecraft position. In addition, it was found that small spacecraft acceleration modeling errors can induce large errors in the Doppler-derived downtrack position estimate.

Atmospheric effects on active illumination

NASA Astrophysics Data System (ADS)

Shaw, Scot E. J.; Kansky, Jan E.

2005-08-01

For some beam-control applications, we can rely on the cooperation of the target when gathering information about the target location and the state of the atmosphere between the target and the beam-control system. The typical example is a cooperative point-source beacon on the target. Light from such a beacon allows the beam-control system to track the target accurately, and, if higher-order adaptive optics is to be employed, to make wave-front measurements and apply appropriate corrections with a deformable mirror. In many applications, including directed-energy weapons, the target is not cooperative. In the absence of a cooperative beacon, we must find other ways to collect the relevant information. This can be accomplished with an active-illumination system. Typically, this means shining one or more lasers at the target and observing the reflected light. In this paper, we qualitatively explore a number of difficulties inherent to active illumination, and suggest some possible mitigation techniques.

A program to compute the two-step excitation of mesospheric sodium atoms for the Polychromatic Laser Guide Star Project

NASA Astrophysics Data System (ADS)

Bellanger, Véronique; Courcelle, Arnaud; Petit, Alain

2004-09-01

A program to compute the two-step excitation of sodium atoms ( 3S→3P→4D) using the density-matrix formalism is presented. The BEACON program calculates population evolution and the number of photons emitted by fluorescence from the 3P, 4D, 4P, 4S levels. Program summaryTitle of program: BEACON Catalogue identifier:ADSX Program Summary URL:http://cpc.cs.qub.ac.uk/cpc/summaries/ADSX Program obtainable from: CPC Program Library, Queen's University of Belfast, N. Ireland Licensing provisions: none Operating systems under which the program has been tested: Win; Unix Programming language used: FORTRAN 77 Memory required to execute with typical data: 1 Mw Number of bits in a word: 32 Number of processors used: 1 (a parallel version of this code is also available and can be obtained on request) Number of lines in distributed program, including test data, etc.: 29 287 Number of bytes in distributed program, including test data, etc.: 830 331 Distribution format: tar.gz CPC Program Library subprograms used: none Nature of physical problem: Resolution of the Bloch equations in the case of the two-step laser excitation of sodium atoms. Method of solution: The program BEACON calculates the evolution of level population versus time using the density-matrix formalism. The number of photons emitted from the 3P, 4D and 4P levels is calculated using the branching ratios and the level lifetimes. Restriction on the complexity of the problem: Since the backscatter emission is calculated after the excitation process, excitation with laser pulse duration longer than the 4D level lifetime cannot be rigorously treated. Particularly, cw laser excitation cannot be calculated with this code. Typical running time:12 h

Aircraft Position Measurement Using Laser Beacon Optics.

DTIC Science & Technology

1981-01-01

8217Comparison of Solar Concentrators .’ Solar Energy, Vol. 10, p. 93, 1976. 7. Winston , R., ’Light Collection Within the Framework of Goemetrical Optics ...8217 J. Optical Society of Am., Vol. 60, p. 245, 1970. 8. Welford, W. T., tics of Nonimaging Concentrators . New York, Academic Press, T978. 9. Bracewell...helicopter pilot and the flight engineer on board the YO-3A. This thesis will concentrate on the development of the laser beacon, the detector optics

Achievements of ATS-6 beacon experiment over Indian sub-continent

NASA Technical Reports Server (NTRS)

Deshpande, M. R.; Rastogi, R. G.; Vats, H. O.; Sethia, G.; Chandra, H.; Davies, K.; Grubb, R. N.; Jones, J. E.

1978-01-01

The repositioning of the ATS-6 satellite at 34 deg E enabled the scientific community of India to use the satellite's radio beacon for ionospheric studies. Two scientific projects were undertaken. The objective of the first project was to map ionospheric electron content, range rate errors, traveling ionospheric phenomena, solar flare effect, and magnetic phenomena. The second project was aimed at studying geophysical phenomena associated with the equatorial electrojet. The principal results of these studies are described.

Dual-channel detection of metallothioneins and mercury based on a mercury-mediated aptamer beacon using thymidine-mercury-thymidine complex as a quencher.

PubMed

Chen, Si-Han; Wang, Yong-Sheng; Chen, Yun-Sheng; Tang, Xian; Cao, Jin-Xiu; Li, Ming-Hui; Wang, Xiao-Feng; Zhu, Yu-Feng; Huang, Yan-Qin

2015-01-01

A novel dual-channel strategy for the detection of metallothioneins (MTs) and Hg(2+) has been developed based on a mercury-mediated aptamer beacon (MAB) using thymidine-mercury-thymidine complex as a quencher for the first time. In the presence of Hg(2+), the T-rich oligonucleotide with a 6-carboxyfluorescein (TRO-FAM) can form an aptamer beacon via the formation of T-Hg(2+)-T base pairs, which results in a fluorescence quenching of the sensing system owing to the fluorescence resonance energy transfer (FRET) from the fluorophore of FAM to the terminated T-Hg(2+)-T base pair. The addition of MTs into this solution leads to the disruption of the T-Hg(2+)-T complex, resulting in an increase of the fluorescent signal of the system. In the optimizing condition, ΔF was directly proportional to the concentrations ranging from 5.63 nM to 0.275 μM for MTs, and 14.2 nM to 0.30 μM for Hg(2+) with the detection limits of 1.69 nM and 4.28 nM, respectively. The proposed dual-channel method avoids the label steps of a quencher in common molecular beacon strategies, without tedious procedure or the requirement of sophisticated equipment, and is rapid, inexpensive and sensitive. Copyright © 2015 Elsevier B.V. All rights reserved.

The CAFADIS camera: a new tomographic wavefront sensor for Adaptive Optics

NASA Astrophysics Data System (ADS)

Rodríguez, J. M.; Femenía, B.; Montilla, I.; Rodríguez-Ramos, L. F.; Marichal-Hernández, J. G.; Lüke, J. P.; López, R.; Díaz, J. J.; Martín, Y.

The CAFADIS camera is a new wavefront sensor (WFS) patented by the Universidad de La Laguna. CAFADIS is a system based on the concept of plenoptic camera originally proposed by Adelson and Wang [Single lens stereo with a plenoptic camera, IEEE Transactions on Pattern Analysis and Machine Intelligence 14 (1992)] and its most salient feature is its ability to simultaneously measuring wavefront maps and distances to objects [Wavefront and distance measurements using the CAFADIS camera, in Astronomical telescopes, Marseille (2008)]. This makes of CAFADIS an interesting alternative for LGS-based AO systems as it is capable of measuring from an LGS-beacon the atmospheric turbulence wavefront and simultaneously the distance to the LGS beacon thus removing the need of a NGS defocus sensor to probe changes in distance to the LGS beacon due to drifts of the mesospheric Na layer. In principle, the concept can also be employed to recover 3D profiles of the Na Layer allowing for optimizations of the measurement of the distance to the LGS-beacon. Currently we are investigating the possibility of extending the plenoptic WFS into a tomographic wavefront sensor. Simulations will be shown of a plenoptic WFS when operated within an LGS-based AO system for the recovery of wavefront maps at different heights. The preliminary results presented here show the tomographic ability of CAFADIS.

United States Air Force Summer Faculty Research Program. 1985 Technical Report. Volume 2.

DTIC Science & Technology

1985-12-01

Voluntary Hand Grip Torque for Dr. Samuel Adams Circular Electrical Connectors 3 Properties and Processing of a Dr. Vernon R. Allen Perfluorinated ...Neutral Particle Beam at Low Energies in the Mark I Aerospace Chamber 126 Preparation of Non-Flammable Model Dr. Terrill D. Smith Compounds 127 Studies on...Synthesis of Azo Compounds F xi 140 A Comparison of Measured and Calculated Dr. Larry Vardiman Attenuation of 28 GHZ Beacon Signals in Three California

Ultranarrowband searches for extraterrestrial intelligence with dedicated signal-processing hardware

NASA Technical Reports Server (NTRS)

Horowitz, P.; Matthews, B. S.; Forster, J.; Linscott, I.; Teague, C. C.; Chen, K.; Backus, P.

1986-01-01

An evaluation is made of the prospects for SETI applications of multichannel spectroscopy, assuming narrowband RF beacon transmission, with a mHz resolution that matches interstellar medium properties. Receiver Doppler corrections must furnish substantial interference rejection. Results are presented from an Arecibo antenna search of 250 sunlike stars at 1.4 and 2.8 GHz. A meridian transit search of the northern sky is also in progress with the Harvard-Smithsonian 26-m antenna.

RNA imaging: tracking in real-time RNA transport in neurons using molecular beacons and confocal microscopy.

PubMed

Zepeda, Angélica; Arias, Clorinda; Flores-Jasso, Fabian; Vaca, Luis

2013-01-01

RNAs are present within eukaryotic cells and are involved in several biological processes. RNA transport within cell compartments is important for proper cell function. To understand in depth the cellular processes in which RNA is involved requires a method that reveals RNA localization in real time in a sub-cellular context in living cells. In this protocol we describe a method for imaging RNA in living cells and in particular in neuronal cultures based on cell microinjection of molecular beacons in conjunction with confocal microscopy. This methodology overcomes some of the main obstacles for imaging RNA in live cells since microinjection allows the delivery of the probe to a desired cellular compartment and MBs bind with high specificity to its target RNA without inhibiting its function. The proper design of the MBs is essential to obtain RNA-MB association at the temperature of the cell cytosol. MBs design with other purposes in mind (such as PCR experiments) have a design that facilitates association to its target at high temperatures, rendering them unsuitable for live cell imaging. Using the methodology described in this chapter allows the study of RNA transport to different regions of neurons and may be combined with the tagging of proteins of interest to measure co-transport of the protein and the RNA to different cellular regions. Copyright © 2013 Elsevier Inc. All rights reserved.

Numerical analysis of hybrid adaptive optics system for correcting beacon anisoplanatism and thermal blooming

NASA Astrophysics Data System (ADS)

Belen'kii, Mikhail S.; Rye, Vincent; Runyeon, Hope

2007-09-01

A concept of a Hybrid Wavefront-based Stochastic Parallel Gradient Decent (WSPGD) Adaptive Optics (AO) system for correcting the combined effects of Beacon Anisoplanatism and Thermal Blooming is introduced. This system integrates a conventional phase conjugate (PC) AO system with a WSPGD AO system. It uses on-axis wavefront measurements of a laser return from an extended beacon to generate initial deformable mirror (DM) commands. Since high frequency phase components are removed from the wavefront of a laser return by a low-pass filter effect of an extended beacon, the system also uses off-axis wavefront measurements to provide feedback for a multi-dithering beam control algorithm in order to generate additional DM commands that account for those missing high frequency phase components. Performance of the Hybrid WSPGD AO system was evaluated in simulation using a wave optics code. Numerical analysis was performed for two tactical scenarios that included ranges of L = 2 km and L = 20 km, ratio of aperture diameter to Fried parameter, D/r 0, of up to 15, ratio of beam spot size at the target to isoplanatic angle, θ B/θ 0, of up to 40, and general distortion number characterizing the strength of Thermal Blooming, N d = 50, 75, and 100. A line-of-sight in the corrected beam was stabilized using a target-plane tracker. The simulation results reveal that the Hybrid WSPGD AO system can efficiently correct the effects of Beacon Anisoplanatism and Thermal Blooming, providing improved compensation of Thermal Blooming in the presence of strong turbulence. Simulation results also indicate that the Hybrid WSPGD AO system outperforms a conventional PC AO system, increasing the Strehl ratio by up to 300% in less than 50 iterations. A follow-on laboratory demonstration performed under a separate program confirmed our theoretical predictions.

Developing children’s palliative care in Africa through beacon centres: lessons learnt

PubMed Central

2013-01-01

Much progress has been made in the provision of palliative care across sub-Saharan Africa, however much still remains to be done, particularly in the area of children’s palliative care (CPC). The Beacon Centres programme was set up in 2009, aimed at improving access to CPC in South Africa, Uganda and Tanzania through more and better-trained health professionals and CPC clinical services of a high standard. Having identified sites in each country to develop into CPC Beacon Centres, Navigators were identified who would be the ‘champions’ for CPC in those sites and lead a programme of training, mentorship and support. Five navigators (2 in Uganda and Tanzania and 1 in South Africa) were trained between September and December 2009. Following this they undertook CPC needs assessments at the 3 centres and set up and delivered a six-month CPC training programme, providing mentorship and support to students to enable them to integrate CPC into their workplaces. To date, 188 participants have commenced the six-month course, with 80 having completed it. CPC has been integrated into the activities of the centres and a CPC virtual resource centre set up in South Africa. The achievements from the Beacon project have been great and the work of the navigators immense, but as in all projects it has not been without its challenges. Lessons learnt include issues around: the focus of the project; the length and nature of the training; assessment; accreditation; the choice of navigators; mentoring; administrative support; co-ordination; the choice of project sites; and the integration of CPC into services. The need for CPC is not going to go away and it is therefore important that models of scaling-up are found that are not only practical, feasible, affordable and sustainable, but that focus on the outcome of improved CPC for all those who need it. It is hoped that the lessons shared from the Beacon Project will help in developing and implementing such models. PMID:23419095

The research of adaptive-exposure on spot-detecting camera in ATP system

NASA Astrophysics Data System (ADS)

Qian, Feng; Jia, Jian-jun; Zhang, Liang; Wang, Jian-Yu

2013-08-01

High precision acquisition, tracking, pointing (ATP) system is one of the key techniques of laser communication. The spot-detecting camera is used to detect the direction of beacon in laser communication link, so that it can get the position information of communication terminal for ATP system. The positioning accuracy of camera decides the capability of laser communication system directly. So the spot-detecting camera in satellite-to-earth laser communication ATP systems needs high precision on target detection. The positioning accuracy of cameras should be better than +/-1μ rad . The spot-detecting cameras usually adopt centroid algorithm to get the position information of light spot on detectors. When the intensity of beacon is moderate, calculation results of centroid algorithm will be precise. But the intensity of beacon changes greatly during communication for distance, atmospheric scintillation, weather etc. The output signal of detector will be insufficient when the camera underexposes to beacon because of low light intensity. On the other hand, the output signal of detector will be saturated when the camera overexposes to beacon because of high light intensity. The calculation accuracy of centroid algorithm becomes worse if the spot-detecting camera underexposes or overexposes, and then the positioning accuracy of camera will be reduced obviously. In order to improve the accuracy, space-based cameras should regulate exposure time in real time according to light intensity. The algorithm of adaptive-exposure technique for spot-detecting camera based on metal-oxide-semiconductor (CMOS) detector is analyzed. According to analytic results, a CMOS camera in space-based laser communication system is described, which utilizes the algorithm of adaptive-exposure to adapting exposure time. Test results from imaging experiment system formed verify the design. Experimental results prove that this design can restrain the reduction of positioning accuracy for the change of light intensity. So the camera can keep stable and high positioning accuracy during communication.

Utilization of space technology for terrestrial solar power applications

NASA Technical Reports Server (NTRS)

Yasui, R. K.; Patterson, R. E.

1974-01-01

A description is given of the evolution of photovoltaic power systems designed and built for terrestrial applications, giving attention to problem areas which are currently impeding the further development of such systems. The rooftop testing of surplus solar panels is considered along with solar powered seismic observatories, solar powered portable radio sets, and design considerations identified from past experience. Present activities discussed are related to a solar powered on-shore beacon flasher system, a solar powered buoy, and a solar powered beacon flasher buoy.

Mountain-Top-to-Mountain-Top Optical Link Demonstration. Part 2

NASA Technical Reports Server (NTRS)

Biswas, A.; Wright, M. W.

2002-01-01

A mountain-top-to-mountain-top optical communications demonstration was conducted between the JPL Table Mountain Facility (TMF), Wrightwood, California, and Strawberry Peak (SP), Lake Arrowhead, California, during the months of August and September of 2000. The link was nearly horizontal at an altitude of 2 km and spanned a range of 46.8 km. A 780-nm multibeam beacon broadcast from TMF was received by the JPL Optical Communications Demonstrator (OCD) located at SP. The received beacon was utilized as a pointing reference to retransmit an 852-nm communications laser beam, modulated at 400 Mb/s by a PN7 pseudo-random bit stream (PRBS) sequence. The long atmospheric path resulted in atmospheric-turbulence-induced angle-of-arrival fluctuations of the beacon at the OCD aperture. A .ne-steering control loop was used to track the resulting beacon centroid motion and update the pointing of the communications laser beam transmitted from SP to TMF. Fried parameters, or r0, inferred from focal spot sizes received at SP were 4 to 5 cm whereas, using the spot sizes received at TMF, they were 2 to 3 cm. In both cases, theory predicts larger r0 values. The predicted angle-of-arrival fluctuations compare well with measured rms displacements exhibited by the focal spots at either end of the link. An uncompensated error of 1.1 rad in the x-axis and 2 rad in the y-axis was obtained using centroid data logged by the OCD. Average bit-error rates of 10-5 were recorded for extended periods of time. An atmospheric coherence length r0 of 3 to 5 cm was inferred using the focal-plane spot size measured on the CCD tracking sensor and compared to a predicted value of 5 to 7 cm using reasonable atmospheric models. The irradiance bounds required for the CCD tracking sensor to perform centroiding was found to range from 2000 to 3000 integrated pixel counts, although a more reliable range was 600 to 3000, indicating a dynamic range of 6 to 11 dB. The motion of the spot on the focal plane was also recorded and yielded rms angle-of-arrival-induced focal-plane displacement of 9 to 11 m, compared to a predicted value of 7.8 to 11 m. The irradiance measurements made with the OCD clearly indicate that best tracking performance is obtained when the mean received signal is 2000 to 2200 counts. The best tracking performance resulted in an rms uncompensated error of 1.1 rad. The uncompensated error appeared to increase with either an increase or decrease in mean beacon-signal level. The rms uncompensated error determined by deriving the beacon displacement power spectral density from the beacon centroid-versustime data and the rejection function of the control loop yielded a higher value of 3.4 rad.

Tracking a head-mounted display in a room-sized environment with head-mounted cameras

NASA Astrophysics Data System (ADS)

Wang, Jih-Fang; Azuma, Ronald T.; Bishop, Gary; Chi, Vernon; Eyles, John; Fuchs, Henry

1990-10-01

This paper presents our efforts to accurately track a Head-Mounted Display (HMD) in a large environment. We review our current benchtop prototype (introduced in {WCF9O]), then describe our plans for building the full-scale system. Both systems use an inside-oui optical tracking scheme, where lateraleffect photodiodes mounted on the user's helmet view flashing infrared beacons placed in the environment. Church's method uses the measured 2D image positions and the known 3D beacon locations to recover the 3D position and orientation of the helmet in real-time. We discuss the implementation and performance of the benchtop prototype. The full-scale system design includes ceiling panels that hold the infrared beacons and a new sensor arrangement of two photodiodes with holographic lenses. In the full-scale system, the user can walk almost anywhere under the grid of ceiling panels, making the working volume nearly as large as the room.

Meteorological factors in Earth-satellite propagation

NASA Technical Reports Server (NTRS)

Levis, C. A.; Taylor, R. C.; Leonard, R.; Lin, K. T.; Pigon, B.; Weller, A.

1982-01-01

Using the COMSTAR D/4 28.56 GHz beacon as a source, a differential gain experiment was performed by connecting a 5-meter paraboloidal antenna and a 0.6-meter paraboloidal antenna alternately to the same receiver. Substantial differential gain changes were observed during some, but not all, rain events. A site-diversity experiment was implemented which consists of two 28.56 GHz radiometers separated by 9 km. The look-angle corresponds to that of the D/4 beacon, and data were obtained with one radiometer during several weeks of concurrent beacon operation to verify the system calibration. A theoretical study of the effect of scattering from a nonuniform rain distribution along the path is under way to aid in interpreting the results of this experiment. An improved empirical site diversity-gain model was derived from data in the literature relating to 34 diversity experiments. Work on the experiment control and data acquisition system is continuing with a view toward future experiments.

Microbial detection with low molecular weight RNA.

PubMed

Kourentzi, K D; Fox, G E; Willson, R C

2001-12-01

The need to monitor microorganisms in the environment has increased interest in assays based on hybridization probes that target nucleic acids (e.g., rRNA). We report the development of liquid-phase assays for specific bacterial 5S rRNA sequences or similarly sized artificial RNAs (aRNAs) using molecular beacon technology. These beacons fluoresce only in the presence of specific target sequences, rendering as much as a 27-fold fluorescence enhancement. The assays can be used with both crude cell lysates and purified total RNA preparations. Minimal sample preparation (e.g., heating to promote leakage from cells) is sufficient to detect many Gram-negative bacteria. Using this approach it was possible to detect an aRNA-labeled Escherichia coli strain in the presence of a large background of an otherwise identical E. coli strain. Finally, by using a longer wavelength carboxytetramethylrhodamine beacon it was possible to reduce the fraction of the signal due to cellular autofluorescence to below 0.5%.

Statistical results from the Virginia Tech propagation experiment using the Olympus 12, 20, and 30 GHz satellite beacons

NASA Technical Reports Server (NTRS)

Stutzman, Warren L.; Safaai-Jazi, A.; Pratt, Timothy; Nelson, B.; Laster, J.; Ajaz, H.

1993-01-01

Virginia Tech has performed a comprehensive propagation experiment using the Olympus satellite beacons at 12.5, 19.77, and 29.66 GHz (which we refer to as 12, 20, and 30 GHz). Four receive terminals were designed and constructed, one terminal at each frequency plus a portable one with 20 and 30 GHz receivers for microscale and scintillation studies. Total power radiometers were included in each terminal in order to set the clear air reference level for each beacon and also to predict path attenuation. More details on the equipment and the experiment design are found elsewhere. Statistical results for one year of data collection were analyzed. In addition, the following studies were performed: a microdiversity experiment in which two closely spaced 20 GHz receivers were used; a comparison of total power and Dicke switched radiometer measurements, frequency scaling of scintillations, and adaptive power control algorithm development. Statistical results are reported.

Topical MMP beacon enabled fluorescence-guided resection of oral carcinoma

PubMed Central

Burgess, Laura; Chen, Juan; Wolter, Nikolaus E.; Wilson, Brian; Zheng, Gang

2016-01-01

Each year almost 300,000 individuals worldwide are diagnosed with oral cancer, more than 90% of these being oral carcinoma [N. Engl. J. Med. 328, 184 19938417385]. Surgical resection is the standard of care, but accurate delineation of the tumor boundaries is challenging, resulting in either under-resection with risk of local recurrence or over-resection with increased functional loss and negative impact on quality of life. This study evaluates, in two pre-clinical in vivo tumor models, the potential of fluorescence-guided resection using molecular beacons activated by metalloproteinases, which are frequently upregulated in human oral cancer. In both models there was rapid (<15 min) beacon activation upon local application, allowing clear fluoresecence imaging in vivo and confirmed by ex vivo fluorescence microscopy and HPLC, with minimal activation in normal oral tissues. Although the tissue penetration was limited using topical application, these findings support further development of this approach towards translation to first-in-human trials. PMID:27231609

Microbial detection with low molecular weight RNA

NASA Technical Reports Server (NTRS)

Kourentzi, K. D.; Fox, G. E.; Willson, R. C.

2001-01-01

The need to monitor microorganisms in the environment has increased interest in assays based on hybridization probes that target nucleic acids (e.g., rRNA). We report the development of liquid-phase assays for specific bacterial 5S rRNA sequences or similarly sized artificial RNAs (aRNAs) using molecular beacon technology. These beacons fluoresce only in the presence of specific target sequences, rendering as much as a 27-fold fluorescence enhancement. The assays can be used with both crude cell lysates and purified total RNA preparations. Minimal sample preparation (e.g., heating to promote leakage from cells) is sufficient to detect many Gram-negative bacteria. Using this approach it was possible to detect an aRNA-labeled Escherichia coli strain in the presence of a large background of an otherwise identical E. coli strain. Finally, by using a longer wavelength carboxytetramethylrhodamine beacon it was possible to reduce the fraction of the signal due to cellular autofluorescence to below 0.5%.

First observations of large-scale wave structure and equatorial spread F using CERTO radio beacon on the C/NOFS satellite

NASA Astrophysics Data System (ADS)

Thampi, Smitha V.; Yamamoto, Mamoru; Tsunoda, Roland T.; Otsuka, Yuichi; Tsugawa, Takuya; Uemoto, Jyunpei; Ishii, Mamoru

2009-09-01

First observations of large-scale wave structure (LSWS) and the subsequent development of equatorial spread F (ESF), using total electron content (TEC) derived from the ground based reception of beacon signals from the CERTO (Coherent Electromagnetic Radio Tomography) radio beacon on board C/NOFS (Communications/Navigation Outage Forecasting System) satellite, are presented. Selected examples of TEC variations, using measurements made during January 2009 from Bac Lieu, Vietnam (9.2°N, 105.6°E geographic, 1.7°N magnetic dip latitude) are presented to illustrate two key findings: (1) LSWS appears to play a more important role in the development of ESF than the post-sunset rise (PSSR) of the F-layer, and (2) LSWS can appear well before E region sunset. Other findings, that LSWS does not have significant zonal drift in the initial stages of growth, and can have zonal wavelengths of several hundred kilometers, corroborate earlier reports.

RNA-templated single-base mutation detection based on T4 DNA ligase and reverse molecular beacon.

PubMed

Tang, Hongxing; Yang, Xiaohai; Wang, Kemin; Tan, Weihong; Li, Huimin; He, Lifang; Liu, Bin

2008-06-15

A novel RNA-templated single-base mutation detection method based on T4 DNA ligase and reverse molecular beacon (rMB) has been developed and successfully applied to identification of single-base mutation in codon 273 of the p53 gene. The discrimination was carried out using allele-specific primers, which flanked the variable position in the target RNA and was ligated using T4 DNA ligase only when the primers perfectly matched the RNA template. The allele-specific primers also carried complementary stem structures with end-labels (fluorophore TAMRA, quencher DABCYL), which formed a molecular beacon after RNase H digestion. One-base mismatch can be discriminated by analyzing the change of fluorescence intensity before and after RNase H digestion. This method has several advantages for practical applications, such as direct discrimination of single-base mismatch of the RNA extracted from cell; no requirement of PCR amplification; performance of homogeneous detection; and easily design of detection probes.

"Signal" search for intelligence in the galactic nucleus with the array of the Lowlands.

PubMed

Shostak, G S; Tarter, J

1985-01-01

In August, 1981, the Westerbork Synthesis Radio Telescope was used for 4 h to search for narrowband pulsing radio beacons in the direction of the Galactic Center. By using both the spatial discrimination and temporal stability available to an interferometric measurement, weak intermittent signals can be detected even in the face of the strong, naturally caused radiation from this region. A radio beacon within our bandwidth, centered on the 21 cm neutral hydrogen line, would be recognizable if it had a repetition period between 40 sec and 1/2 h. The rms sensitivity to point sources was approximately 50 mJy/cycle, and the detection limit was 500 mJy/cycle. The limit degrades for pulse widths < 0.02s. No repetitive signals were found. For a swept, narrow-band radio beacon constrained to the Galactic Disk (beamwidth = 0.02 rad), our detection limit corresponds to a transmitter power of 10(11) MW at the Galactic Center.

Tracking Data Acquisition System (TDAS) for the 1990's. Volume 6: TDAS navigation system architecture

NASA Technical Reports Server (NTRS)

Elrod, B. D.; Jacobsen, A.; Cook, R. A.; Singh, R. N. P.

1983-01-01

One-way range and Doppler methods for providing user orbit and time determination are examined. Forward link beacon tracking, with on-board processing of independent navigation signals broadcast continuously by TDAS spacecraft; forward link scheduled tracking; with on-board processing of navigation data received during scheduled TDAS forward link service intervals; and return link scheduled tracking; with ground-based processing of user generated navigation data during scheduled TDAS return link service intervals are discussed. A system level definition and requirements assessment for each alternative, an evaluation of potential navigation performance and comparison with TDAS mission model requirements is included. TDAS satellite tracking is also addressed for two alternatives: BRTS and VLBI tracking.

A method for optical ground station reduce alignment error in satellite-ground quantum experiments

NASA Astrophysics Data System (ADS)

He, Dong; Wang, Qiang; Zhou, Jian-Wei; Song, Zhi-Jun; Zhong, Dai-Jun; Jiang, Yu; Liu, Wan-Sheng; Huang, Yong-Mei

2018-03-01

A satellite dedicated for quantum science experiments, has been developed and successfully launched from Jiuquan, China, on August 16, 2016. Two new optical ground stations (OGSs) were built to cooperate with the satellite to complete satellite-ground quantum experiments. OGS corrected its pointing direction by satellite trajectory error to coarse tracking system and uplink beacon sight, therefore fine tracking CCD and uplink beacon optical axis alignment accuracy was to ensure that beacon could cover the quantum satellite in all time when it passed the OGSs. Unfortunately, when we tested specifications of the OGSs, due to the coarse tracking optical system was commercial telescopes, the change of position of the target in the coarse CCD was up to 600μrad along with the change of elevation angle. In this paper, a method of reduce alignment error between beacon beam and fine tracking CCD is proposed. Firstly, OGS fitted the curve of target positions in coarse CCD along with the change of elevation angle. Secondly, OGS fitted the curve of hexapod secondary mirror positions along with the change of elevation angle. Thirdly, when tracking satellite, the fine tracking error unloaded on the real-time zero point position of coarse CCD which computed by the firstly calibration data. Simultaneously the positions of the hexapod secondary mirror were adjusted by the secondly calibration data. Finally the experiment result is proposed. Results show that the alignment error is less than 50μrad.

Navigation Architecture for a Space Mobile Network

NASA Technical Reports Server (NTRS)

Valdez, Jennifer E.; Ashman, Benjamin; Gramling, Cheryl; Heckler, Gregory W.; Carpenter, Russell

2016-01-01

The Tracking and Data Relay Satellite System (TDRSS) Augmentation Service for Satellites (TASS) is a proposed beacon service to provide a global, space based GPS augmentation service based on the NASA Global Differential GPS (GDGPS) System. The TASS signal will be tied to the GPS time system and usable as an additional ranging and Doppler radiometric source. Additionally, it will provide data vital to autonomous navigation in the near Earth regime, including space weather information, TDRS ephemerides, Earth Orientation Parameters (EOP), and forward commanding capability. TASS benefits include enhancing situational awareness, enabling increased autonomy, and providing near real-time command access for user platforms. As NASA Headquarters' Space Communication and Navigation Office (SCaN) begins to move away from a centralized network architecture and towards a Space Mobile Network (SMN) that allows for user initiated services, autonomous navigation will be a key part of such a system. This paper explores how a TASS beacon service enables the Space Mobile Networking paradigm, what a typical user platform would require, and provides an in-depth analysis of several navigation scenarios and operations concepts. This paper provides an overview of the TASS beacon and its role within the SMN and user community. Supporting navigation analysis is presented for two user mission scenarios: an Earth observing spacecraft in low earth orbit (LEO), and a highly elliptical spacecraft in a lunar resonance orbit. These diverse flight scenarios indicate the breadth of applicability of the TASS beacon for upcoming users within the current network architecture and in the SMN.

Application of quantum-dots for analysis of nanosystems by either utilizing or preventing FRET

NASA Astrophysics Data System (ADS)

Kim, Joong H.; Chaudhary, Sumit; Stephens, Jared P.; Singh, Krishna V.; Ozkan, Mihrimah

2005-04-01

We have developed conjugates with quantum-dots (QDs) for the purpose of analysis of nanosystems that are organic or inorganic in nature such as DNA and carbon nanotubes. First, by employing Florescence Resonant Energy Transfer (FRET) principles, a hybrid molecular beacon conjugates are synthesized. For water- solubilization of QDs, we modified the surface of CdSe-ZnS core-shell QD by using mercaptoacetic acid ligand. This modification does not affect the size of QDs from that of unmodified QDs. After linking molecular beacons to the carboxyl groups of the modified QDs using 1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride, hybrid molecular beacons are prepared as a DNA probe. After hybridization with specific target DNA and non-specific target DNA, the hybrid conjugates show high specificity to the target DNA with 5-fold increase in the intensity of fluorescence. By developing atomic model of the conjugates, we calculated with 8 numbers of molecular beacons on a single quantum dots, we could increase the efficiency of FRET up to 90%. In other hands, for application of quantum dots to the carbon nanotubes, FRET is a barrier. Thus, after employing 1 % sodium-dodecyl-sulfonate (SDS), single-walled carbon nanotubes are decorated with QDs at their outer surface. This enables fluorescent microscopy imaging of single-walled carbon nanotubes which is a more common technique than electron microscopy. In summary, QDs can be used for analysis or detection of both organic and inorganic based nanosystems.

A Machine Learning Approach to Predict Gene Regulatory Networks in Seed Development in Arabidopsis

PubMed Central

Ni, Ying; Aghamirzaie, Delasa; Elmarakeby, Haitham; Collakova, Eva; Li, Song; Grene, Ruth; Heath, Lenwood S.

2016-01-01

Gene regulatory networks (GRNs) provide a representation of relationships between regulators and their target genes. Several methods for GRN inference, both unsupervised and supervised, have been developed to date. Because regulatory relationships consistently reprogram in diverse tissues or under different conditions, GRNs inferred without specific biological contexts are of limited applicability. In this report, a machine learning approach is presented to predict GRNs specific to developing Arabidopsis thaliana embryos. We developed the Beacon GRN inference tool to predict GRNs occurring during seed development in Arabidopsis based on a support vector machine (SVM) model. We developed both global and local inference models and compared their performance, demonstrating that local models are generally superior for our application. Using both the expression levels of the genes expressed in developing embryos and prior known regulatory relationships, GRNs were predicted for specific embryonic developmental stages. The targets that are strongly positively correlated with their regulators are mostly expressed at the beginning of seed development. Potential direct targets were identified based on a match between the promoter regions of these inferred targets and the cis elements recognized by specific regulators. Our analysis also provides evidence for previously unknown inhibitory effects of three positive regulators of gene expression. The Beacon GRN inference tool provides a valuable model system for context-specific GRN inference and is freely available at https://github.com/BeaconProjectAtVirginiaTech/beacon_network_inference.git. PMID:28066488

A gold nanoparticles-modified aptamer beacon for urinary adenosine detection based on structure-switching/fluorescence-"turning on" mechanism.

PubMed

Zhang, Jin-Quan; Wang, Yong-Sheng; Xue, Jin-Hua; He, Yan; Yang, Hui-Xian; Liang, Jun; Shi, Lin-Fei; Xiao, Xi-Lin

2012-11-01

A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis. The Ade aptamer was engineered into an aptamer beacon by adding a gold nanoparticle-modified nucleotide sequence which is complementary to aptamer sequence (FDNA) at the 3'-end of FDNA. The fluorescence signal "turning on" was observed when AB was bound to Ade, which is attributed to a significant conformational change in AB from a FDNA/QDNA duplex to a FDNA-Ade complex. The Ade measurement was carried out in 20 mmol L(-1) Tris-HCl buffer solution of pH 7.4, ΔF signal linearly correlated with the concentration of Ade over the range of 2.0×10(-8) to 1.8×10(-6) mol L(-1). The limit of detection (LOD) for Ade is 6.0×10(-9) mol L(-1) with relative standard deviations (R.S.D) of 3.64-5.36%, and the recoveries were 98.6%, 100%, 102% (n=6), respectively. The present method has been successfully applied to determine Ade in human urine samples, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the unique properties of the AB could provide a promising potential for small molecules detection, and be benefit to extend the application of aptamer beacon technique. Copyright © 2012 Elsevier B.V. All rights reserved.

Beacon: a web portal to high-quality mental health websites for use by health professionals and the public.

PubMed

Christensen, Helen; Murray, Kristen; Calear, Alison L; Bennett, Kylie; Bennett, Anthony; Griffiths, Kathleen M

2010-06-07

To describe the Beacon web portal, which lists and rates quality health websites, collects user characteristics and publishes user feedback; and to report summary data on Beacon's highest-rated (best evidence-based) sites for mental health. A systematic search was undertaken in February 2009 for potentially relevant websites through a review of research papers and a recently published book, an internet search of Open Directory Project medical categories, a review of material on a high-profile mental health portal, and a survey of international researchers. All sites were ranked on a 7-point scale from -1 to 5, with negative scores indicating evidence of no effect and scores of 2 or more indicating evidence of efficacy based on reports in the scientific literature. By March 2010, 183 sites had been identified, of which 122 focused on physical health or wellbeing, 40 targeted anxiety, and 23 targeted depression. Of the eight generalised anxiety disorder sites identified, four achieved ratings of 2 or above. Two social anxiety disorder sites achieved scores higher than 2. Ten panic disorder sites were identified, with three achieving ratings of 2 or above; and five post-traumatic stress disorder sites were identified, with two achieving ratings of 2 or above. Of the 23 identified depression sites, four achieved a rating of 2 or above. There are a number of high-quality mental health websites on the internet, and Beacon provides a portal to enable the wide dissemination of these resources.

Validation of Air Traffic Controller Workload Models

DTIC Science & Technology

1979-09-01

15 2.2 ATF Description ................................. 20 2.3 Summary of Changes to RECEP/ATF ............... 22 2.3.1 Definition of Routine...as an exhavistive descrip- tion. Emphasis is placed more on methodology while highlighting areas where changes in the process have oeen made as a... Change 3.7 2.2 Beacon (Discrete) Code 3.3 2.3 Mode C Altitude Report 2.9 2.0 A/C Position Report 3.0 2.6 A/C Altitude Report 2.0 2.6 Heading and Speed

Telemetry link for an automatic salmon migration monitor

NASA Technical Reports Server (NTRS)

Baldwin, H. A.; Freyman, R. W.

1973-01-01

The antenna and transmitter described in this report were designed for integration into the remote acoustic assessment system for detection of sockeye salmon in the Bristol Bay region of the Bering Sea. The assessment system configuration consists of an upward directed sonar buoy anchored 150 ft below the surface and attached by cable to a spar buoy tethered some 300 ft laterally. The spar buoy contains a telemetry transmitter, power supply, data processing electronics, an antenna and a beacon light.

Investment Criteria for Airport Surveillance Radar, Air Traffic Control Radar Beacon System, and Automated Radar Terminal System (ASR/ATCRBS/ARTS)

DTIC Science & Technology

1983-05-01

15 FIQGX 4 Tine Saved By ASR: 3 Mile vs. 7.5 Mile Separation 40 3 4 S10" 6 ;9 q4, ItI I ILI 10! i~o 2 _ _P𔃻_ _ P_ _ IFR HUM 16 :,.4eS _OE_ AT_...8217 16 . Abstract ""This report develops revised investment criteria for Airport Survoiillance Radar, Air Traffic Control Radar Beacon System, and...12 A. Introduction. .................... 1. 32 B. IFR Delay Reduction Benefits ...... ............. 13 C. Safety Benefits

Indoor Pedestrian Localization Using iBeacon and Improved Kalman Filter.

PubMed

Sung, Kwangjae; Lee, Dong Kyu 'Roy'; Kim, Hwangnam

2018-05-26

The reliable and accurate indoor pedestrian positioning is one of the biggest challenges for location-based systems and applications. Most pedestrian positioning systems have drift error and large bias due to low-cost inertial sensors and random motions of human being, as well as unpredictable and time-varying radio-frequency (RF) signals used for position determination. To solve this problem, many indoor positioning approaches that integrate the user's motion estimated by dead reckoning (DR) method and the location data obtained by RSS fingerprinting through Bayesian filter, such as the Kalman filter (KF), unscented Kalman filter (UKF), and particle filter (PF), have recently been proposed to achieve higher positioning accuracy in indoor environments. Among Bayesian filtering methods, PF is the most popular integrating approach and can provide the best localization performance. However, since PF uses a large number of particles for the high performance, it can lead to considerable computational cost. This paper presents an indoor positioning system implemented on a smartphone, which uses simple dead reckoning (DR), RSS fingerprinting using iBeacon and machine learning scheme, and improved KF. The core of the system is the enhanced KF called a sigma-point Kalman particle filter (SKPF), which localize the user leveraging both the unscented transform of UKF and the weighting method of PF. The SKPF algorithm proposed in this study is used to provide the enhanced positioning accuracy by fusing positional data obtained from both DR and fingerprinting with uncertainty. The SKPF algorithm can achieve better positioning accuracy than KF and UKF and comparable performance compared to PF, and it can provide higher computational efficiency compared with PF. iBeacon in our positioning system is used for energy-efficient localization and RSS fingerprinting. We aim to design the localization scheme that can realize the high positioning accuracy, computational efficiency, and energy efficiency through the SKPF and iBeacon indoors. Empirical experiments in real environments show that the use of the SKPF algorithm and iBeacon in our indoor localization scheme can achieve very satisfactory performance in terms of localization accuracy, computational cost, and energy efficiency.

Dual-band beacon experiment over Southeast Asia for ionospheric irregularity analysis

NASA Astrophysics Data System (ADS)

Watthanasangmechai, K.; Yamamoto, M.; Saito, A.; Saito, S.; Maruyama, T.; Tsugawa, T.; Nishioka, M.

2013-12-01

An experiment of dual-band beacon over Southeast Asia was started in March 2012 in order to capture and analyze ionospheric irregularities in equatorial region. Five GNU Radio Beacon Receivers (GRBRs) were aligned along 100 degree geographic longitude. The distances between the stations reach more than 500 km. The field of view of this observational network covers +/- 20 degree geomagnetic latitude including the geomagnetic equator. To capture ionospheric irregularities, the absolute TEC estimation technique was developed. The two-station method (Leitinger et al., 1975) is generally accepted as a suitable method to estimate TEC offsets of dual-band beacon experiment. However, the distances between the stations directly affect on the robustness of the technique. In Southeast Asia, the observational network is too sparse to attain a benefit of the classic two-station method. Moreover, the least-squares approch used in the two-station method tries too much to adjust the small scales of the TEC distribution which are the local minima. We thus propose a new technique to estimate the TEC offsets with the supporting data from absolute GPS-TEC from local GPS receivers and the ionospheric height from local ionosondes. The key of the proposed technique is to utilize the brute-force technique with weighting function to find the TEC offset set that yields a global minimum of RMSE in whole parameter space. The weight is not necessary when the TEC distribution is smooth, while it significantly improves the TEC estimation during the ESF events. As a result, the latitudinal TEC shows double-hump distribution because of the Equatorial Ionization Anomaly (EIA). In additions, the 100km-scale fluctuations from an Equatorial Spread F (ESF) are captured at night time in equinox seasons. The plausible linkage of the meridional wind with triggering of ESF is under invatigating and will be presented. The proposed method is successful to estimate the latitudinal TEC distribution from dual-band frequency beacon data for the sparse observational network in Southeast Asia which may be useful for other equatorial sectors like Affrican region as well.

Simultaneous and multiplexed detection of exosome microRNAs using molecular beacons.

PubMed

Lee, Ji Hye; Kim, Jeong Ah; Jeong, Seunga; Rhee, Won Jong

2016-12-15

Simultaneous and multiplexed detection of microRNAs (miRNAs) in a whole exosome is developed, which can be utilized as a PCR-free efficient diagnosis method for various diseases. Exosomes are small extracellular vesicles that contain biomarker miRNAs from parental cells. Because they circulate throughout bodily fluids, exosomal biomarkers offer great advantages for diagnosis in many aspects. In general, PCR-based methods can be used for exosomal miRNA detection but they are laborious, expensive, and time-consuming, which make them unsuitable for high-throughput diagnosis of diseases. Previously, we reported that single miRNA in the exosomes can be detected specifically using an oligonucleotide probe or molecular beacon. Herein, we demonstrate for the first time that multiple miRNAs can be detected simultaneously in exosomes using miRNA-targeting molecular beacons. Exosomes from a breast cancer cell line, MCF-7, were used for the production of exosomes because MCF-7 has a high level of miR-21, miR-375, and miR-27a as target miRNAs. Molecular beacons successfully hybridized with multiple miRNAs in the cancer cell-derived exosomes even in the presence of high human serum concentration. In addition, it is noteworthy that the choice of fluorophores for multiplexing biomarkers in an exosome is crucial because of its small size. The proposed method described in this article is beneficial to high-throughput analysis for disease diagnosis, prognosis, and response to treatment because it is a time-, labor-, and cost-saving technique. Copyright © 2016 Elsevier B.V. All rights reserved.

Performance of the NASA Beacon Receiver for the Alphasat Aldo Paraboni TDP5 Propagation Experiment

NASA Technical Reports Server (NTRS)

Nessel, James; Morse, Jacquelynne; Zemba, Michael; Riva, Carlo; Luini, Lorenzo

2015-01-01

NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 gigahertz band. NASA GRC has developed and installed a K/Q-band (20/40 gigahertz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20/40 gigahertz signals broadcast from the Alphasat Aldo Paraboni Technology Demonstration Payload (TDP) no. 5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we describe the design and preliminary performance of the NASA propagation terminal, which has been installed and operating in Milan since June 2014. The receiver is based upon a validated Fast Fourier Transform (FFT) I/Q digital design approach utilized in other operational NASA propagation terminals, but has been modified to employ power measurement via a frequency estimation technique and to coherently track and measure the amplitude of the 20/40 gigahertz beacon signals. The system consists of a 1.2-meter K-band and a 0.6-meter Q-band Cassegrain reflector employing synchronous open-loop tracking to track the inclined orbit of the Alphasat satellite. An 8 hertz sampling rate is implemented to characterize scintillation effects, with a 1-hertz measurement bandwidth dynamic range of 45 decibels. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models.

A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design.

PubMed

Zhou, Zhenpeng; Huang, Hongduan; Chen, Yang; Liu, Feng; Huang, Cheng Zhi; Li, Na

2014-02-15

A new metal-enhanced fluorescence (MEF) based platform was developed on the basis of distance-dependent fluorescence quenching-enhancement effect, which combined the easiness of Ag-thiol chemistry with the MEF property of noble-metal structures as well as the molecular beacon design. For the given sized AgNPs, the fluorescence enhancement factor was found to increase with a d(6) dependency in agreement with fluorescence resonance energy transfer mechanism at shorter distance and decrease with a d(-3) dependency in agreement with plasmonic enhancement mechanism at longer distance between the fluorophore and the AgNP surface. As a proof of concept, the platform was demonstrated by a sensitive detection of mercuric ions, using thymine-containing molecular beacon to tune silver nanoparticle (AgNP)-enhanced fluorescence. Mercuric ions were detected via formation of a thymine-mercuric-thymine structure to open the hairpin, facilitating fluorescence recovery and AgNP enhancement to yield a limit of detection of 1 nM, which is well below the U.S. Environmental Protection Agency regulation of the Maximum Contaminant Level Goal (10nM) in drinking water. Since the AgNP functioned as not only a quencher to reduce the reagent blank signal but also an enhancement substrate to increase fluorescence of the open hairpin when target mercuric ions were present, the quenching-enhancement strategy can greatly improve the detection sensitivity and can in principle be a universal approach for various targets when combined with molecular beacon design. © 2013 Elsevier B.V. All rights reserved.

Implications of directed energy for SETI

NASA Astrophysics Data System (ADS)

Lubin, Philip

2016-09-01

We compute the detectability of directed-energy (DE) sources from distant civilizations that may exist. Recent advances in our own DE technology suggest that our eventual capabilities will radically enhance our capacity to broadcast our presence and hence allow us to ponder the reverse case of detection. We show that DE systems are detectable at vast distances, possibly across the entire horizon, which profoundly alters conceivable search strategies for extra-terrestrial, technologically-advanced civilizations. Even modest searches are extremely effective at detecting or constraining many civilization classes. A single civilization anywhere in our galaxy of comparable technological advancement to our own can be detected with near unity probability with a cluster of 0.1 m telescopes on Earth. A 1 m class telescope can detect a single civilization anywhere in the Andromeda galaxy. A search strategy is proposed using small Earth-based telescopes to observe 1012-1020 stellar and planetary systems. Such observations could address whether there exist other civilizations which are broadcasting with similar or more advanced DE capability. We show that such searches have near-unity probability of detecting comparably advanced civilizations anywhere in our galaxy within a few years, assuming the civilization: (1) adopts a simple "intelligent targeting" beacon strategy; (2) is beaconing at a wavelength we can detect; (3) broadcast the beacon long enough for the light to reach Earth now. In this blind-beacon, blind-search strategy, the civilization need not know where we are nor do we need to know where they are. The same basic strategy can be extended to extragalactic distances.

Preliminary Results of the NASA Beacon Receiver for Alphasat Aldo Paraboni TDP5 Propagation Experiment

NASA Technical Reports Server (NTRS)

Nessel, James; Morse, Jacquelynne; Zemba, Michael; Riva, Carlo; Luini, Lorenzo

2014-01-01

NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 GHz band. NASA GRC has developed and installed a K/Q-band (20/40 GHz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20/40 GHz signals broadcast from the Alphasat Aldo Paraboni TDP#5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we describe the design and preliminary performance of the NASA propagation terminal, which has been installed and operating in Milan since May 2014. The receiver is based upon a validated Fast Fourier Transform (FFT) I/Q digital design approach utilized in other operational NASA propagation terminals, but has been modified to employ power measurement via a frequency estimation technique and to coherently track and measure the amplitude of the 20/40 GHz beacon signals. The system consists of a 1.2-m K-band and a 0.6-m Qband Cassegrain reflector employing synchronous open-loop tracking to track the inclined orbit of the Alphasat satellite. An 8 Hz sampling rate is implemented to characterize scintillation effects, with a 1-Hz measurement bandwidth dynamic range of 45 dB. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models.

Development of a Molecular-Beacon Assay To Detect the G1896A Precore Mutation in Hepatitis B Virus-Infected Individuals

PubMed Central

Waltz, Therese L.; Marras, Salvatore; Rochford, Gemma; Nolan, John; Lee, Eugenia; Melegari, Margherita; Pollack, Henry

2005-01-01

The 1896 precore (PC) mutation is the most frequent cause of hepatitis B virus e-antigen (HBeAg)-negative chronic hepatitis B virus (HBV) infection. Detection of the 1896 PC mutation has application in studies monitoring antiviral therapy and the natural history of the disease. Identification of this mutation is usually performed by direct sequencing, which is both costly and laborious. The aim of this study was to develop a rapid, high-throughput assay to detect the 1896 PC mutation using real-time PCR and molecular-beacon technology. The assay was initially standardized on oligonucleotide targets and plasmids containing the wild-type (WT) and PC mutation and then tested on plasma samples from children with HBV DNA of >106 copies/ml. Nine individuals were HBeAg negative and suspected to harbor HBeAg mutations, while 12 children were HBeAg positive and selected as controls. Ninety percent (19 of 21) of plasma samples tested with molecular beacons were in complete agreement with sequencing results. The remaining 10% (2 of 21) of samples were identified as heterogeneous mixtures of WT and mutant virus by molecular beacons, though sequencing found only a homogeneous mutant in both cases. Overall, the 1896 PC mutation was detected by this assay in 55.5% of the children with HBeAg-negative infection. In summary, this assay is a rapid, sensitive, and specific technique that effectively discriminates WT from 1896 PC mutant HBV and may be useful in clinical and epidemiological studies. PMID:15634980

Making it local: Beacon Communities use health information technology to optimize care management.

PubMed

Allen, Amy; Des Jardins, Terrisca R; Heider, Arvela; Kanger, Chatrian R; Lobach, David F; McWilliams, Lee; Polello, Jennifer M; Rein, Alison L; Schachter, Abigail A; Singh, Ranjit; Sorondo, Barbara; Tulikangas, Megan C; Turske, Scott A

2014-06-01

Care management aims to provide cost-effective, coordinated, non-duplicative care to improve care quality, population health, and reduce costs. The 17 communities receiving funding from the Office of the National Coordinator for Health Information Technology through the Beacon Community Cooperative Agreement Program are leaders in building and strengthening their health information technology (health IT) infrastructure to provide more effective and efficient care management. This article profiles 6 Beacon Communities' health IT-enabled care management programs, highlighting the influence of local context on program strategy and design, and describing challenges, lessons learned, and policy implications for care delivery and payment reform. The unique needs (eg, disease burden, demographics), community partnerships, and existing resources and infrastructure all exerted significant influence on the overall priorities and design of each community's care management program. Though each Beacon Community needed to engage in a similar set of care management tasks--including patient identification, stratification, and prioritization; intervention; patient engagement; and evaluation--the contextual factors helped shape the specific strategies and tools used to carry out these tasks and achieve their objectives. Although providers across the country are striving to deliver standardized, high-quality care, the diverse contexts in which this care is delivered significantly influence the priorities, strategies, and design of community-based care management interventions. Gaps and challenges in implementing effective community-based care management programs include: optimizing allocation of care management services; lack of available technology tailored to care management needs; lack of standards and interoperability; integrating care management into care settings; evaluating impact; and funding and sustainability.

The ANTARES optical beacon system

NASA Astrophysics Data System (ADS)

Ageron, M.; Aguilar, J. A.; Albert, A.; Ameli, F.; Anghinolfi, M.; Anton, G.; Anvar, S.; Ardellier-Desages, F.; Aslanides, E.; Aubert, J.-J.; Auer, R.; Barbarito, E.; Basa, S.; Battaglieri, M.; Becherini, Y.; Beltramelli, J.; Bertin, V.; Bigi, A.; Billault, M.; Blaes, R.; de Botton, N.; Bouwhuis, M. C.; Bradbury, S. M.; Bruijn, R.; Brunner, J.; Burgio, G. F.; Busto, J.; Cafagna, F.; Caillat, L.; Calzas, A.; Capone, A.; Caponetto, L.; Carmona, E.; Carr, J.; Cartwright, S. L.; Castel, D.; Castorina, E.; Cavasinni, V.; Cecchini, S.; Ceres, A.; Charvis, P.; Chauchot, P.; Chiarusi, T.; Circella, M.; Colnard, C.; Compère, C.; Coniglione, R.; Cottini, N.; Coyle, P.; Cuneo, S.; Cussatlegras, A.-S.; Damy, G.; van Dantzig, R.; de Bonis, G.; de Marzo, C.; de Vita, R.; Dekeyser, I.; Delagnes, E.; Denans, D.; Deschamps, A.; Destelle, J.-J.; Dinkespieler, B.; Distefano, C.; Donzaud, C.; Drogou, J.-F.; Druillole, F.; Durand, D.; Ernenwein, J.-P.; Escoffier, S.; Falchini, E.; Favard, S.; Fehr, F.; Feinstein, F.; Ferry, S.; Fiorello, C.; Flaminio, V.; Fratini, K.; Fuda, J.-L.; Galeotti, S.; Gallone, J.-M.; Giacomelli, G.; Girard, N.; Gojak, C.; Goret, Ph.; Graf, K.; Hallewell, G.; Harakeh, M. N.; Hartmann, B.; Heijboer, A.; Heine, E.; Hello, Y.; Hernández-Rey, J. J.; Hößl, J.; Hoffman, C.; Hogenbirk, J.; Hubbard, J. R.; Jaquet, M.; Jaspers, M.; de Jong, M.; Jouvenot, F.; Kalantar-Nayestanaki, N.; Kappes, A.; Karg, T.; Katz, U.; Keller, P.; Kok, E.; Kok, H.; Kooijman, P.; Kopper, C.; Korolkova, E. V.; Kouchner, A.; Kretschmer, W.; Kruijer, A.; Kuch, S.; Kudryavstev, V. A.; Lagier, P.; Lahmann, R.; Lamanna, G.; Lamare, P.; Lambard, G.; Languillat, J.-C.; Laschinsky, H.; Lavalle, J.; Le Guen, Y.; Le Provost, H.; Le van Suu, A.; Lefèvre, D.; Legou, T.; Lelaizant, G.; Lim, G.; Lo Presti, D.; Loehner, H.; Loucatos, S.; Louis, F.; Lucarelli, F.; Lyashuk, V.; Marcelin, M.; Margiotta, A.; Masullo, R.; Mazéas, F.; Mazure, A.; McMillan, J. E.; Megna, R.; Melissas, M.; Migneco, E.; Milovanovic, A.; Mongelli, M.; Montaruli, T.; Morganti, M.; Moscoso, L.; Musumeci, M.; Naumann-Godo, M.; Naumann, C.; Niess, V.; Noble, T.; Olivetto, C.; Ostasch, R.; Palanque-Delabrouille, N.; Payre, P.; Peek, H.; Perez, A.; Petta, C.; Piattelli, P.; Pillet, R.; Pineau, J.-P.; Poinsignon, J.; Popa, V.; Pradier, T.; Racca, C.; Randazzo, N.; van Randwijk, J.; Real, D.; van Rens, B.; Réthoré, F.; Rewiersma, P.; Riccobene, G.; Rigaud, V.; Ripani, M.; Roca, V.; Roda, C.; Rolin, J. F.; Rose, H. J.; Rostovtsev, A.; Roux, J.; Ruppi, M.; Russo, G. V.; Rusydi, G.; Salesa, F.; Salomon, K.; Sapienza, P.; Schmitt, F.; Schuller, J.-P.; Shanidze, R.; Sokalski, I.; Spona, T.; Spurio, M.; van der Steenhoven, G.; Stolarczyk, T.; Streeb, K.; Sulak, L.; Taiuti, M.; Tamburini, C.; Tao, C.; Terreni, G.; Thompson, L. F.; Urbano, F.; Valdy, P.; Valente, V.; Vallage, B.; Vaudaine, G.; Venekamp, G.; Verlaat, B.; Vernin, P.; de Vries-Uiterweerd, G.; van Wijk, R.; Wijnker, G.; de Witt Huberts, P.; Wobbe, G.; de Wolf, E.; Yao, A.-F.; Zaborov, D.; Zaccone, H.; Zornoza, J. D.; Zúñiga, J.

2007-08-01

ANTARES is a neutrino telescope being deployed in the Mediterranean Sea. It consists of a three-dimensional array of photomultiplier tubes that can detect the Cherenkov light induced by charged particles produced in the interactions of neutrinos with the surrounding medium. High angular resolution can be achieved, in particular, when a muon is produced, provided that the Cherenkov photons are detected with sufficient timing precision. Considerations of the intrinsic time uncertainties stemming from the transit time spread in the photomultiplier tubes and the mechanism of transmission of light in sea water lead to the conclusion that a relative time accuracy of the order of 0.5 ns is desirable. Accordingly, different time calibration systems have been developed for the ANTARES telescope. In this article, a system based on Optical Beacons, a set of external and well-controlled pulsed light sources located throughout the detector, is described. This calibration system takes into account the optical properties of sea water, which is used as the detection volume of the ANTARES telescope. The design, tests, construction and first results of the two types of beacons, LED and laser-based, are presented.

GPS-Like Phasing Control of the Space Solar Power System Transmission Array

NASA Technical Reports Server (NTRS)

Psiaki, Mark L.

2003-01-01

The problem of phasing of the Space Solar Power System's transmission array has been addressed by developing a GPS-like radio navigation system. The goal of this system is to provide power transmission phasing control for each node of the array that causes the power signals to add constructively at the ground reception station. The phasing control system operates in a distributed manner, which makes it practical to implement. A leader node and two radio navigation beacons are used to control the power transmission phasing of multiple follower nodes. The necessary one-way communications to the follower nodes are implemented using the RF beacon signals. The phasing control system uses differential carrier phase relative navigation/timing techniques. A special feature of the system is an integer ambiguity resolution procedure that periodically resolves carrier phase cycle count ambiguities via encoding of pseudo-random number codes on the power transmission signals. The system is capable of achieving phasing accuracies on the order of 3 mm down to 0.4 mm depending on whether the radio navigation beacons operate in the L or C bands.

Studying the influence of stem composition in pH-sensitive molecular beacons onto their sensing properties.

PubMed

Dembska, Anna; Kierzek, Elzbieta; Juskowiak, Bernard

2017-10-16

Intracellular sensing using fluorescent molecular beacons is a potentially useful strategy for real-time, in vivo monitoring of important cellular events. This work is focused on evaluation of pyrene excimer signaling molecular beacons (MBs) for the monitoring of pH changes in vitro as well as inside living cells. The recognition element in our MB called pHSO (pH-sensitive oligonucleotide) is the loop enclosing cytosine-rich fragment that is able to form i-motif structure in a specific pH range. However, alteration of a sequence of the 6 base pairs containing stem of MB allowed the design of pHSO probes that exhibited different dynamic pH range and possessed slightly different transition midpoint between i-motif and open loop configuration. Moreover, this conformational transition was accompanied by spectral changes showing developed probes different pyrene excimer-monomer emission ratio triggered by pH changes. The potential of these MBs for intracellular pH sensing is demonstrated on the example of HeLa cells line. Copyright © 2017 Elsevier B.V. All rights reserved.

Rotating stellar populations in the Fornax dSph galaxy

NASA Astrophysics Data System (ADS)

del Pino, Andrés; Aparicio, Antonio; Hidalgo, Sebastian L.; Łokas, Ewa L.

2017-03-01

We present a novel analysis of the internal kinematics of the Fornax dwarf spheroidal galaxy. Our results are based on the largest sample of spectroscopic data for Fornax stars presently available (>2500 stars), for which we have chemical and kinematic information. We introduce new software, BEACON, designed to detect chemo-kinematic patterns among stars of different stellar populations using their metallicity and velocity along the line of sight. Applying BEACON to Fornax, we have detected non-negligible rotation signals around main optical axes of the galaxy, characteristic for a triaxial system partially supported by rotation. The dominant rotation pattern is relatively strong (∼12 km s-1), but the galaxy also shows additional weaker albeit complex rotation patterns. Using the information available from the star formation history of Fornax, we have also derived the average age of the different chemo-kinematic components found by BEACON, which has allowed us to obtain its kinematic history. Our results point to a possible major merger suffered by Fornax at redshift z ∼ 1, in agreement with the previous works.

Collaborative Localization Algorithms for Wireless Sensor Networks with Reduced Localization Error

PubMed Central

Sahoo, Prasan Kumar; Hwang, I-Shyan

2011-01-01

Localization is an important research issue in Wireless Sensor Networks (WSNs). Though Global Positioning System (GPS) can be used to locate the position of the sensors, unfortunately it is limited to outdoor applications and is costly and power consuming. In order to find location of sensor nodes without help of GPS, collaboration among nodes is highly essential so that localization can be accomplished efficiently. In this paper, novel localization algorithms are proposed to find out possible location information of the normal nodes in a collaborative manner for an outdoor environment with help of few beacons and anchor nodes. In our localization scheme, at most three beacon nodes should be collaborated to find out the accurate location information of any normal node. Besides, analytical methods are designed to calculate and reduce the localization error using probability distribution function. Performance evaluation of our algorithm shows that there is a tradeoff between deployed number of beacon nodes and localization error, and average localization time of the network can be increased with increase in the number of normal nodes deployed over a region. PMID:22163738

Molecular beacons for DNA binding proteins: an emerging technology for detection of DNA binding proteins and their ligands.

PubMed

Dummitt, Benjamin; Chang, Yie-Hwa

2006-06-01

Quantitation of the level or activity of specific proteins is one of the most commonly performed experiments in biomedical research. Protein detection has historically been difficult to adapt to high throughput platforms because of heavy reliance upon antibodies for protein detection. Molecular beacons for DNA binding proteins is a recently developed technology that attempts to overcome such limitations. Protein detection is accomplished using inexpensive, easy-to-synthesize oligonucleotides, accompanied by a fluorescence readout. Importantly, detection of the protein and reporting of the signal occur simultaneously, allowing for one-step protocols and increased potential for use in high throughput analysis. While the initial iteration of the technology allowed only for the detection of sequence-specific DNA binding proteins, more recent adaptations allow for the possibility of development of beacons for any protein, independent of native DNA binding activity. Here, we discuss the development of the technology, the mechanism of the reaction, and recent improvements and modifications made to improve the assay in terms of sensitivity, potential for multiplexing, and broad applicability.

ARGOS: the laser guide star system for the LBT

NASA Astrophysics Data System (ADS)

Rabien, S.; Ageorges, N.; Barl, L.; Beckmann, U.; Blümchen, T.; Bonaglia, M.; Borelli, J. L.; Brynnel, J.; Busoni, L.; Carbonaro, L.; Davies, R.; Deysenroth, M.; Durney, O.; Elberich, M.; Esposito, S.; Gasho, V.; Gässler, W.; Gemperlein, H.; Genzel, R.; Green, R.; Haug, M.; Hart, M. L.; Hubbard, P.; Kanneganti, S.; Masciadri, E.; Noenickx, J.; Orban de Xivry, G.; Peter, D.; Quirrenbach, A.; Rademacher, M.; Rix, H. W.; Salinari, P.; Schwab, C.; Storm, J.; Strüder, L.; Thiel, M.; Weigelt, G.; Ziegleder, J.

2010-07-01

ARGOS is the Laser Guide Star adaptive optics system for the Large Binocular Telescope. Aiming for a wide field adaptive optics correction, ARGOS will equip both sides of LBT with a multi laser beacon system and corresponding wavefront sensors, driving LBT's adaptive secondary mirrors. Utilizing high power pulsed green lasers the artificial beacons are generated via Rayleigh scattering in earth's atmosphere. ARGOS will project a set of three guide stars above each of LBT's mirrors in a wide constellation. The returning scattered light, sensitive particular to the turbulence close to ground, is detected in a gated wavefront sensor system. Measuring and correcting the ground layers of the optical distortions enables ARGOS to achieve a correction over a very wide field of view. Taking advantage of this wide field correction, the science that can be done with the multi object spectrographs LUCIFER will be boosted by higher spatial resolution and strongly enhanced flux for spectroscopy. Apart from the wide field correction ARGOS delivers in its ground layer mode, we foresee a diffraction limited operation with a hybrid Sodium laser Rayleigh beacon combination.

GPS-Squitter capacity analysis

NASA Astrophysics Data System (ADS)

Orlando, Vincent A.; Harman, William H.

1994-05-01

GPS-Squitter is a system concept that merges the capabilities of Automatic Dependent Surveillance (ADS) and the Mode S beacon radar. The result is an integrated concept for seamless surveillance and data link that permits equipped aircraft to participate in ADS and/or beacon ground environments. This concept offers many possibilities for transition from a beacon to an ADS-based environment. This report provides the details of the techniques used to estimate GPS-Squitter surveillance and data link capacity. Surveillance capacity of airborne aircraft is calculated for the omni and six-sector ground stations. Next, the capacity of GPS-Squitter for surface traffic is estimated. The interaction between airborne and surface operations is addressed to show the independence of these systems. Air ground data link capacity for GPS-Squitter is estimated, together with an estimate of the use of the Mode S link to support other ground surveillance and data link activities as well as TCAS operation. The analysis indicates the low transponder occupancy resulting from the total effect of these activities. Low occupancy is a key requirement in avoiding interference with the operation of the current ATCRBS and future Mode S interrogators.

Two-Photon-Absorption Scheme for Optical Beam Tracking

NASA Technical Reports Server (NTRS)

Ortiz, Gerardo G.; Farr, William H.

2011-01-01

A new optical beam tracking approach for free-space optical communication links using two-photon absorption (TPA) in a high-bandgap detector material was demonstrated. This tracking scheme is part of the canonical architecture described in the preceding article. TPA is used to track a long-wavelength transmit laser while direct absorption on the same sensor simultaneously tracks a shorter-wavelength beacon. The TPA responsivity was measured for silicon using a PIN photodiode at a laser beacon wavelength of 1,550 nm. As expected, the responsivity shows a linear dependence with incident power level. The responsivity slope is 4.5 x 10(exp -7) A/W2. Also, optical beam spots from the 1,550-nm laser beacon were characterized on commercial charge coupled device (CCD) and complementary metal-oxide semiconductor (CMOS) imagers with as little as 13.7 microWatts of optical power (see figure). This new tracker technology offers an innovative solution to reduce system complexity, improve transmit/receive isolation, improve optical efficiency, improve signal-to-noise ratio (SNR), and reduce cost for free-space optical communications transceivers.

Preliminary Statistics from the NASA Alphasat Beacon Receiver in Milan, Italy

NASA Technical Reports Server (NTRS)

Zemba, Michael J.; Nessel, James A.; Morse, Jacquelynne R.

2015-01-01

NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 GHz band. NASA GRC has developed and installed a KQ-band (20-40 GHz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20-40 GHz signals broadcast from the Alphasat Aldo Paraboni TDP5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we provide an overview of the design and data calibration procedure, and present 6 months of preliminary statistics of the NASA propagation terminal, which has been installed and operating in Milan since May 2014. The Q-band receiver has demonstrated a dynamic range of 40 dB at an 8-Hz sampling rate. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models

Preliminary Statistics from the NASA Alphasat Beacon Receiver in Milan, Italy

NASA Technical Reports Server (NTRS)

Nessel, James; Zemba, Michael; Morse, Jacquelynne; Luini, Lorenzo; Riva, Carlo

2015-01-01

NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 gigahertz band. NASA GRC has developed and installed a K/Q-band (20/40 gigahertz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20/40 gigahertz signals broadcast from the Alphasat Aldo Paraboni TDP no. 5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we provide an overview of the design and data calibration procedure, and present 6 months of preliminary statistics of the NASA propagation terminal, which has been installed and operating in Milan since May 2014. The Q-band receiver has demonstrated a dynamic range of 40 decibels at an 8-hertz sampling rate. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models.

20 MW Flywheel frequency regulation plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arseneaux, James

Hazle designed, built, commissioned, and operates a utility-scale 20 MW flywheel energy storage plant in Hazle Township, Pennsylvania (the Hazle Facility) using flywheel technology developed by its affiliate, Beacon Power, LLC (Beacon Power). The Hazle Facility provides frequency regulation services to the regional transmission organization, PJM Interconnection, LLC (PJM), through its participation in PJM’s Regulation Market (a market-based system for the purchase and sale of the Regulation ancillary service). The zero emission Hazle Facility is designed for a 20 year-life over which it is capable of performing at least 100,000 full depth of discharge cycles. To achieve its 20 MWmore » capacity, the Hazle Facility is comprised of two hundred of Beacon Power’s 100 kilowatt (kW)/25 kilowatt/hour (kWh) flywheels connected in parallel. The Hazle Facility can fully respond to a signal from PJM in less than 2 seconds. The Hazle facility was constructed in an economic development zone designated by the Commonwealth of Pennsylvania and its construction relied on local contractors and labor for completion.« less

ATS-6 - Radio Beacon Experiment: The first years. [ionospheric and satellite-to-ground electron content

NASA Technical Reports Server (NTRS)

Davies, K.; Fritz, R. B.; Grubb, R. N.; Jones, J. E.

1975-01-01

The Radio Beacon Experiment aboard Applications Technology Satellite-6 (ATS-6) is designed to measure the total electron content and the ionospheric electron content between the satellite and ground. The spaceborne beacon transmits signals on frequencies of 40, 140, and 360 MHz with amplitude modulations of 1 MHz and/or 0.1 MHz for the measurement of modulation phase, Faraday rotation, and amplitude. The modulation phase delays are calibrated in the satellite and in the ground equipment, and the polarization of the emitted signals are predetermined by standard antenna range techniques. The design of the ATS-6 receiver in Boulder, Colorado, is discussed. The antennae are of the short backfire type described by Ehrenspeck (1967), with nominal gains of 13, 19, and 22 dB at 40, 140, and 360 MHz, respectively. Data recording and overall supervision of the receiver is carried out by a 16-bit minicomputer with 8 k of memory. Overall performance of the system is satisfactory. Sample data on the monthly median hourly values of the total electron content, plasmospheric content, and shape factor show distinct seasonal and diurnal variations.

Power sources for search and rescue 406 MHz beacons

NASA Technical Reports Server (NTRS)

Attia, Alan I.; Perrone, David E.

1987-01-01

The results of a study directed at the selection of a commercially available, safe, low cost, light weight and long storage life battery for search and rescue (Sarsat) 406 MHz emergency beacons are presented. In the course of this work, five electrochemical systems (lithium-manganese dioxide, lithium-carbon monofluoride, lithium-silver vanadium oxide, alkaline cells, and cadmium-mercuric oxide) were selected for limited experimental studies to determine their suitability for this application. Two safe, commercially available batteries (lithium-manganese dioxide and lithium-carbon monofluoride) which meet the near term requirements and several alternatives for the long term were identified.

A Fluorescence Quenching Assay Based on Molecular Beacon Formation through a Ligase Detection Reaction for Facile and Rapid Detection of Point Mutations.

PubMed

Sawamura, Kensuke; Hashimoto, Masahiko

2017-01-01

A fluorescence quenching assay based on a ligase detection reaction was developed for facile and rapid detection of point mutations present in a mixed population of non-variant DNA. If the test DNA carried a targeted mutation, then the two allele-specific primers were ligated to form a molecular beacon resulting in the expected fluorescence quenching signatures. Using this method, we successfully detected as low as 5% mutant DNA in a mixture of wild-type DNA (t test at 99% confidence level).

Flight Validation of On-Demand Operations: The Deep Space One Beacon Monitor Operations Experiment

NASA Technical Reports Server (NTRS)

Wyatt, Jay; Sherwood, Rob; Sue, Miles; Szijjarto, John

2000-01-01

After a brief overview of the operational concept, this paper will provide a detailed description of the _as-flown_ flight software components, the DS1 experiment plan, and experiment results to date. Special emphasis will be given to experiment results and lessons learned since the basic system design has been previously reported. Mission scenarios where beacon operations is highly applicable will be described. Detailed cost savings estimates for a sample science mission will be provided as will cumulative savings that are possible over the next fifteen years of NASA missions.

Recognition of dual targets by a molecular beacon-based sensor: subtyping of influenza A virus.

PubMed

Lee, Chun-Ching; Liao, Yu-Chieh; Lai, Yu-Hsuan; Lee, Chang-Chun David; Chuang, Min-Chieh

2015-01-01

A molecular beacon (MB)-based sensor to offer a decisive answer in combination with information originated from dual-target inputs is designed. The system harnesses an assistant strand and thermodynamically favored designation of unpaired nucleotides (UNs) to process the binary targets in "AND-gate" format and report fluorescence in "off-on" mechanism via a formation of a DNA four-way junction (4WJ). By manipulating composition of the UNs, the dynamic fluorescence difference between the binary targets-coexisting circumstance and any other scenario was maximized. Characteristic equilibrium constant (K), change of entropy (ΔS), and association rate constant (k) between the association ("on") and dissociation ("off") states of the 4WJ were evaluated to understand unfolding behavior of MB in connection to its sensing capability. Favorable MB and UNs were furthermore designed toward analysis of genuine genetic sequences of hemagglutinin (HA) and neuraminidase (NA) in an influenza A H5N2 isolate. The MB-based sensor was demonstrated to yield a linear calibration range from 1.2 to 240 nM and detection limit of 120 pM. Furthermore, high-fidelity subtyping of influenza virus was implemented in a sample of unpurified amplicons. The strategy opens an alternative avenue of MB-based sensors for dual targets toward applications in clinical diagnosis.

Trifunctional molecular beacon-mediated quadratic amplification for highly sensitive and rapid detection of mercury(II) ion with tunable dynamic range.

PubMed

Zhao, Yue; Liu, Huaqing; Chen, Feng; Bai, Min; Zhao, Junwu; Zhao, Yongxi

2016-12-15

Analyses of target with low abundance or concentration varying over many orders of magnitude are severe challenges faced by numerous assay methods due to their modest sensitivity and limited dynamic range. Here, we introduce a homogeneous and rapid quadratic polynomial amplification strategy through rational design of a trifunctional molecular beacon, which serves as not only a reporter molecule but also a bridge to couple two stage amplification modules without adding any reaction components or process other than basic linear amplification. As a test bed for our studies, we took mercury(II) ion as an example and obtained a high sensitivity with detection limit down to 200 pM within 30min. In order to create a tunable dynamic range, homotropic allostery is employed to modulate the target specific binding. When the number of metal binding site varies from 1 to 3, signal response is programmed accordingly with useful dynamic range spanning 50, 25 and 10 folds, respectively. Furthermore, the applicability of the proposed method in river water and biological samples are successfully verified with good recovery and reproducibility, indicating considerable potential for its practicality in complex real samples. Copyright © 2016 Elsevier B.V. All rights reserved.

Approach and Evaluation of a Mobile Video-Based and Location-Based Augmented Reality Platform for Information Brokerage

NASA Astrophysics Data System (ADS)

Dastageeri, H.; Storz, M.; Koukofikis, A.; Knauth, S.; Coors, V.

2016-09-01

Providing mobile location-based information for pedestrians faces many challenges. On one hand the accuracy of localisation indoors and outdoors is restricted due to technical limitations of GPS and Beacons. Then again only a small display is available to display information as well as to develop a user interface. Plus, the software solution has to consider the hardware characteristics of mobile devices during the implementation process for aiming a performance with minimum latency. This paper describes our approach by including a combination of image tracking and GPS or Beacons to ensure orientation and precision of localisation. To communicate the information on Points of Interest (POIs), we decided to choose Augmented Reality (AR). For this concept of operations, we used besides the display also the acceleration and positions sensors as a user interface. This paper especially goes into detail on the optimization of the image tracking algorithms, the development of the video-based AR player for the Android platform and the evaluation of videos as an AR element in consideration of providing a good user experience. For setting up content for the POIs or even generate a tour we used and extended the Open Geospatial Consortium (OGC) standard Augmented Reality Markup Language (ARML).

Mechanisms contributing to adverse cardiovascular events in patients with type 2 diabetes mellitus and stage 4 chronic kidney disease treated with bardoxolone methyl.

PubMed

Chin, Melanie P; Reisman, Scott A; Bakris, George L; O'Grady, Megan; Linde, Peter G; McCullough, Peter A; Packham, David; Vaziri, Nosratola D; Ward, Keith W; Warnock, David G; Meyer, Colin J

2014-01-01

Bardoxolone methyl, an Nrf2-activating and nuclear factor-κB-inhibiting semisynthetic oleanane triterpenoid compound, was evaluated in a phase 3 trial (BEACON) in patients with type 2 diabetes mellitus (T2DM) and stage 4 chronic kidney disease (CKD). The trial was terminated because of an increase in heart failure events in the bardoxolone methyl group, many of which appeared related to fluid retention. Thus, additional analyses were conducted to explain these serious adverse events. Patients (n = 2,185) were randomized to receive once-daily bardoxolone methyl (20 mg) or placebo. Twenty-four-hour urine collections were analyzed in a subset of the BEACON population and from a separate, open-label pharmacology study in patients with stage 3b/4 CKD and T2DM administered 20 mg bardoxolone methyl once daily for 56 consecutive days. Bardoxolone-methyl-treated patients in the BEACON substudy had a clinically meaningful reduction in urine volume and sodium excretion at week 4 relative to baseline (p < 0.05), and a separate study revealed that decreased sodium excretion and urine output occurred in some patients with stage 4 CKD but not those with stage 3b CKD. The clinical phenotype of fluid overload and heart failure in BEACON was similar to that observed with endothelin receptor antagonists in advanced CKD patients, and preclinical data demonstrate that bardoxolone methyl modifies endothelin signaling. The totality of the evidence suggests that through modulation of the endothelin pathway, bardoxolone methyl may pharmacologically promote acute sodium and volume retention and increase blood pressure in patients with more advanced CKD.

Novel Pulse Oximetry Sonifications for Neonatal Oxygen Saturation Monitoring: A Laboratory Study.

PubMed

Hinckfuss, Kelly; Sanderson, Penelope; Loeb, Robert G; Liley, Helen G; Liu, David

2016-03-01

We aimed to test whether the use of novel pulse oximetry sounds (sonifications) better informs listeners when a neonate's oxygen saturation (SpO2) deviates from the recommended range. Variable-pitch pulse oximeters do not accurately inform clinicians via sound alone when SpO2 is outside the target range of 90% to 95% for neonates on supplemental oxygen. Risk of blindness, organ damage, and death increase if SpO2 remains outside the target range. A more informative sonification may improve clinicians' ability to maintain the target range. In two desktop experiments, nonclinicians' ability to detect SpO2 range and direction of change was tested with novel versus conventional sonifications of simulated patient data. In Experiment 1, a "shoulder" sonification used larger pitch differences between adjacent saturation percentages for SpO2 values outside the target range. In Experiment 2, a "beacon" sonification used equal-appearing pitch differences, but when SpO2 was outside the target range, a fixed-pitch reference tone from the center of the target SpO2 range preceded every fourth pulse tone. The beacon sonification improved range identification accuracy over the control display (85% vs. 60%; p < .001), but the shoulder sonification did not (55% vs. 52%). The beacon provided a distinct auditory alert and reference that significantly improved nonclinical participants' ability to identify SpO2 range. Adding a beacon to the variable-pitch pulse oximeter sound may help clinicians identify when, and by how much, a neonate's SpO2 deviates from the target range, particularly during patient transport situations when auditory information becomes essential. © 2015, Human Factors and Ergonomics Society.

Fluorometric determination of nucleic acids based on the use of polydopamine nanotubes and target-induced strand displacement amplification.

PubMed

Ge, Jia; Bai, Dong-Mei; -Geng, Xin; Hu, Ya-Lei; Cai, Qi-Yong; Xing, Ke; Zhang, Lin; Li, Zhao-Hui

2018-01-10

The authors describe a fluorometric method for the quantitation of nucleic acids by combining (a) cycled strand displacement amplification, (b) the unique features of the DNA probe SYBR Green, and (c) polydopamine nanotubes. SYBR Green undergoes strong fluorescence enhancement upon intercalation into double-stranded DNA (dsDNA). The polydopamine nanotubes selectively adsorb single-stranded DNA (ssDNA) and molecular beacons. In the absence of target DNA, the molecular beacon, primer and SYBR Green are adsorbed on the surface of polydopamine nanotubes. This results in quenching of the fluorescence of SYBR Green, typically measured at excitation/emission wavelengths of 488/518 nm. Upon addition of analyte (target DNA) and polymerase, the stem of the molecular beacon is opened so that it can bind to the primer. This triggers target strand displacement polymerization, during which dsDNA is synthesized. The hybridized target is then displaced due to the strand displacement activity of the polymerase. The displaced target hybridizes with another molecular beacon. This triggers the next round of polymerization. Consequently, a large amount of dsDNA is formed which is detected by addition of SYBR Green. Thus, sensitive and selective fluorometric detection is realized. The fluorescent sensing strategy shows very good analytical performances towards DNA detection, such as a wide linear range from 0.05 to 25 nM with a low limit of detection of 20 pM. Graphical abstract Schematic of a fluorometric strategy for highly sensitive and selective determination of nucleic acids by combining strand displacement amplification and the unique features of SYBR Green I (SG) and polydopamine nanotubes.

Making It Local: Beacon Communities Use Health Information Technology to Optimize Care Management

PubMed Central

Allen, Amy; Des Jardins, Terrisca R.; Heider, Arvela; Kanger, Chatrian R.; Lobach, David F.; McWilliams, Lee; Polello, Jennifer M.; Schachter, Abigail A.; Singh, Ranjit; Sorondo, Barbara; Tulikangas, Megan C.; Turske, Scott A.

2014-01-01

Abstract Care management aims to provide cost-effective, coordinated, non-duplicative care to improve care quality, population health, and reduce costs. The 17 communities receiving funding from the Office of the National Coordinator for Health Information Technology through the Beacon Community Cooperative Agreement Program are leaders in building and strengthening their health information technology (health IT) infrastructure to provide more effective and efficient care management. This article profiles 6 Beacon Communities' health IT-enabled care management programs, highlighting the influence of local context on program strategy and design, and describing challenges, lessons learned, and policy implications for care delivery and payment reform. The unique needs (eg, disease burden, demographics), community partnerships, and existing resources and infrastructure all exerted significant influence on the overall priorities and design of each community's care management program. Though each Beacon Community needed to engage in a similar set of care management tasks—including patient identification, stratification, and prioritization; intervention; patient engagement; and evaluation—the contextual factors helped shape the specific strategies and tools used to carry out these tasks and achieve their objectives. Although providers across the country are striving to deliver standardized, high-quality care, the diverse contexts in which this care is delivered significantly influence the priorities, strategies, and design of community-based care management interventions. Gaps and challenges in implementing effective community-based care management programs include: optimizing allocation of care management services; lack of available technology tailored to care management needs; lack of standards and interoperability; integrating care management into care settings; evaluating impact; and funding and sustainability. (Population Health Management 2014;17:149–158) PMID:24476558

Prolonged survival in patients with breast cancer and a history of brain metastases: results of a preplanned subgroup analysis from the randomized phase III BEACON trial.

PubMed

Cortés, Javier; Rugo, Hope S; Awada, Ahmad; Twelves, Chris; Perez, Edith A; Im, Seock-Ah; Gómez-Pardo, Patricia; Schwartzberg, Lee S; Diéras, Veronique; Yardley, Denise A; Potter, David A; Mailliez, Audrey; Moreno-Aspitia, Alvaro; Ahn, Jin-Seok; Zhao, Carol; Hoch, Ute; Tagliaferri, Mary; Hannah, Alison L; O'Shaughnessy, Joyce

2017-09-01

Conventional chemotherapy has limited activity in patients with breast cancer and brain metastases (BCBM). Etirinotecan pegol (EP), a novel long-acting topoisomerase-1 inhibitor, was designed using advanced polymer technology to preferentially accumulate in tumor tissue including brain metastases, providing sustained cytotoxic SN38 levels. The phase 3 BEACON trial enrolled 852 women with heavily pretreated locally recurrent or metastatic breast cancer between 2011 and 2013. BEACON compared EP with treatment of physician's choice (TPC; eribulin, vinorelbine, gemcitabine, nab-paclitaxel, paclitaxel, ixabepilone, or docetaxel) in patients previously treated with anthracycline, taxane, and capecitabine, including those with treated, stable brain metastases. The primary endpoint, overall survival (OS), was assessed in a pre-defined subgroup of BCBM patients; an exploratory post hoc analysis adjusting for the diagnosis-specific graded prognostic assessment (GPA) index was also conducted. In the trial, 67 BCBM patients were randomized (EP, n = 36; TPC, n = 31). Treatment subgroups were balanced for baseline characteristics and GPA indices. EP was associated with a significant reduction in the risk of death (HR 0.51; P < 0.01) versus TPC; median OS was 10.0 and 4.8 months, respectively. Improvement in OS was observed in both poorer and better GPA prognostic groups. Survival rates at 12 months were 44.4% for EP versus 19.4% for TPC. Consistent with the overall BEACON population, fewer patients on EP experienced grade ≥3 toxicity (50 vs. 70%). The significant improvement in survival in BCBM patients provides encouraging data for EP in this difficult-to-treat subgroup of patients. A phase three trial of EP in BCBM patients is underway (ClinicalTrials.gov NCT02915744).

Applications of detrital geochronology and thermochronology from glacial deposits to the Paleozoic and Mesozoic thermal history of the Ross Embayment, Antarctica

NASA Astrophysics Data System (ADS)

Welke, Bethany; Licht, Kathy; Hennessy, Andrea; Hemming, Sidney; Pierce Davis, Elizabeth; Kassab, Christine

2016-07-01

Till from moraines at the heads of six major outlet glaciers in the Transantarctic Mountains (TAM) and from till beneath three West Antarctic ice streams have a ubiquitous zircon U-Pb age population spanning the time of the Ross/Pan-African Orogenies (610-475 Ma). Geochronology and thermochronology of detrital minerals in these Antarctic glacial tills reveal two different thermal histories for the central and southern TAM. Double-dating of the zircons reveals a geographically widespread (U-Th)/He (ZHe) population of 180-130 Ma in most of the till samples. Sandstone outcrops at Shackleton Glacier, and three Beacon Supergroup sandstone clasts from three moraines, have ZHe ages that fall entirely within this range. The similar population and proximity of many of the till samples to Beacon outcrops lead us to suggest that this extensive ZHe population in the tills is derived from Beacon Supergroup rocks and reflects the thermal response of the Beacon Basin to the breakup of Gondwana. A second population of older (>200 Ma) ZHe ages in tills at the head of Byrd, Nimrod, and Reedy Glaciers. For the tills at the head of the Nimrod and Byrd Glaciers, integrating the double-dated zircon results with 40Ar/39Ar of hornblende, muscovite and biotite, and U-Pb and (U-Th-Sm)/He double-dates on apatite yields a typical pattern of early rapid orogenic cooling (˜4-10°C/Myr) 590-475 Ma after the emplacement of the Granite Harbour Intrusives. Low temperature thermochronometers at these sites yield variable but quite old ages (ZHe 480-70 Ma and AHe 200-70 Ma) that require a long history at low temperature.

Evaluation of the Tindouf Basin Region in Southern Morocco as an Analog Site for Soil Geochemistry on Noachian Mars.

PubMed

Oberlin, Elizabeth A; Claire, Mark W; Kounaves, Samuel P

2018-02-09

Locations on Earth that provide insights into processes that may be occurring or may have occurred throughout martian history are often broadly deemed "Mars analog environments." As no single locale can precisely represent a past or present martian environment, it is important to focus on characterization of terrestrial processes that produce analogous features to those observed in specific regions of Mars or, if possible, specific time periods during martian history. Here, we report on the preservation of ionic species in soil samples collected from the Tindouf region of Morocco and compare them with the McMurdo Dry Valleys of Antarctica, the Atacama Desert in Chile, the martian meteorite EETA79001, and the in situ Mars analyses from the Phoenix Wet Chemistry Laboratory (WCL). The Moroccan samples show the greatest similarity with those from Victoria Valley, Beacon Valley, and the Atacama, while being consistently depleted compared to University Valley and enriched compared to Taylor Valley. The NO 3 /Cl ratios are most similar to Victoria Valley and Atacama, while the SO 4 /Cl ratios are similar to those from Beacon Valley, Victoria Valley, and the Atacama. While perchlorate concentrations in the Moroccan samples are typically lower than those found in samples of other analog sites, conditions in the region are sufficiently arid to retain oxychlorines at detectable levels. Our results suggest that the Tindouf Basin in Morocco can serve as a suitable analogue for the soil geochemistry and subsequent aridification of the Noachian epoch on Mars. Key Words: Mars analogues-Antarctica-Morocco-Oxyanions-Perchlorate-Nitrate. Astrobiology 18, xxx-xxx.

Position determination systems. [using orbital antenna scan of celestial bodies

NASA Technical Reports Server (NTRS)

Shores, P. W. (Inventor)

1976-01-01

A system for an orbital antenna, operated at a synchronous altitude, to scan an area of a celestial body is disclosed. The antenna means comprises modules which are operated by a steering signal in a repetitive function for providing a scanning beam over the area. The scanning covers the entire area in a pattern and the azimuth of the scanning beam is transmitted to a control station on the celestial body simultaneous with signals from an activated ground beacon on the celestial body. The azimuth of the control station relative to the antenna is known and the location of the ground beacon is readily determined from the azimuth determinations.

Non-linear molecular pattern classification using molecular beacons with multiple targets.

PubMed

Lee, In-Hee; Lee, Seung Hwan; Park, Tai Hyun; Zhang, Byoung-Tak

2013-12-01

In vitro pattern classification has been highlighted as an important future application of DNA computing. Previous work has demonstrated the feasibility of linear classifiers using DNA-based molecular computing. However, complex tasks require non-linear classification capability. Here we design a molecular beacon that can interact with multiple targets and experimentally shows that its fluorescent signals form a complex radial-basis function, enabling it to be used as a building block for non-linear molecular classification in vitro. The proposed method was successfully applied to solving artificial and real-world classification problems: XOR and microRNA expression patterns. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

The phase of the crosspolarized signal generated by millimeter wave propagation through rain

NASA Technical Reports Server (NTRS)

Overstreet, W. P.; Bostian, C. W.

1978-01-01

Proposed schemes for cancelling rain-induced crosstalk in dual-polarized communications systems depend upon the phase relationships between the wanted and unwanted signals. This report investigates the phase relationship of the rain-generated crosspolarized signal relative to the copolarized signal. Theoretical results obtained from a commonly accepted propagation model are presented. Experimental data from the Communications Technology Satellite beacon and from the Comstar beacon are presented and the correlation between theory and data is discussed. An inexpensive semi-adaptive cancellation system is proposed and its performance expectations are presented. The implications of phase variations on a cancellation system are also discussed.

The 40 and 50 GHz propagation experiments at the Rutherford Appleton Laboratory, UK, using the ITALSAT beacons

NASA Technical Reports Server (NTRS)

Woodroffe, J. M.; Davies, P. G.; Ladd, D. N.; Norbury, John R.

1994-01-01

This paper describes the current experimental program and future plans for the reception of transmissions from the 18.7, 39.6, and 49.5 GHz beacons from the ITALSAT satellite by the Radio Communications Research Unit at Rutherford Appleton Laboratory, UK. The Radio Communications Research Unit, which has had considerable experience in developing experimental millimetric equipment for propagation studies, has initiated the development of a single-channel receiver and a triple-channel receiver to measure propagation effects at 49.5 GHz and 39.6 GHz respectively. The initial location of the receivers will be at Chilbolton, Hampshire, UK.

Method to improve the survival of night-swarming mayflies near bridges in areas of distracting light pollution

PubMed Central

Száz, Dénes; Farkas, Alexandra; Pereszlényi, Ádám; Kriska, György

2017-01-01

Numerous negative ecological effects of urban lighting have been identified during the last decades. In spite of the development of lighting technologies, the detrimental effect of this form of light pollution has not declined. Several insect species are affected including the night-swarming mayfly Ephoron virgo: when encountering bridges during their mass swarming, these mayflies often fall victim to artificial lighting. We show a simple method for the conservation of these mayflies exploiting their positive phototaxis. With downstream-facing light-emitting diode beacon lights above two tributaries of the river Danube, we managed to guide egg-laying females to the water and prevent them from perishing outside the river near urban lights. By means of measuring the mayfly outflow from the river as a function of time and the on/off state of the beacons, we showed that the number of mayflies exiting the river's area was practically zero when our beacons were operating. Tributaries could be the sources of mayfly recolonization in case of water quality degradation of large rivers. The protection of mayfly populations in small rivers and safeguarding their aggregation and oviposition sites is therefore important. PMID:29291103

A distributed transmit beamforming synchronization strategy for multi-element radar systems

NASA Astrophysics Data System (ADS)

Xiao, Manlin; Li, Xingwen; Xu, Jikang

2017-02-01

The distributed transmit beamforming has recently been discussed as an energy-effective technique in wireless communication systems. A common ground of various techniques is that the destination node transmits a beacon signal or feedback to assist source nodes to synchronize signals. However, this approach is not appropriate for a radar system since the destination is a non-cooperative target of an unknown location. In our paper, we propose a novel synchronization strategy for a distributed multiple-element beamfoming radar system. Source nodes estimate parameters of beacon signals transmitted from others to get their local synchronization information. The channel information of the phase propagation delay is transmitted to nodes via the reflected beacon signals as well. Next, each node generates appropriate parameters to form a beamforming signal at the target. Transmit beamforming signals of all nodes will combine coherently at the target compensating for different propagation delay. We analyse the influence of the local oscillation accuracy and the parameter estimation errors on the performance of the proposed synchronization scheme. The results of numerical simulations illustrate that this synchronization scheme is effective to enable the transmit beamforming in a distributed multi-element radar system.

Secure Utilization of Beacons and UAVs in Emergency Response Systems for Building Fire Hazard

PubMed Central

Seo, Seung-Hyun; Choi, Jung-In; Song, Jinseok

2017-01-01

An intelligent emergency system for hazard monitoring and building evacuation is a very important application area in Internet of Things (IoT) technology. Through the use of smart sensors, such a system can provide more vital and reliable information to first-responders and also reduce the incidents of false alarms. Several smart monitoring and warning systems do already exist, though they exhibit key weaknesses such as a limited monitoring coverage and security, which have not yet been sufficiently addressed. In this paper, we propose a monitoring and emergency response method for buildings by utilizing beacons and Unmanned Aerial Vehicles (UAVs) on an IoT security platform. In order to demonstrate the practicability of our method, we also implement a proof of concept prototype, which we call the UAV-EMOR (UAV-assisted Emergency Monitoring and Response) system. Our UAV-EMOR system provides the following novel features: (1) secure communications between UAVs, smart sensors, the control server and a smartphone app for security managers; (2) enhanced coordination between smart sensors and indoor/outdoor UAVs to expand real-time monitoring coverage; and (3) beacon-aided rescue and building evacuation. PMID:28946659

Diagnosis of Neisseria gonorrhoeae Using Molecular Beacon

PubMed Central

Patel, Achchhe Lal; Sonkar, Subash Chandra; Kumari, Indu; Saluja, Daman

2015-01-01

Neisseria gonorrhoeae is an important sexually transmitted diseases (STD) causing pathogen worldwide. Due to absence of an affordable diagnostic assay, routine screening of gonococcal infection becomes impossible in developing countries where infection rates are maximum. Treatment is given on the basis of symptoms alone which leads to spread of infection. Thus, development of a rapid, sensitive, specific, and PCR based visual diagnostic assay suitable for developing countries, required for better disease management, is aimed at in present study. Endocervical swabs were collected from patients visiting gynecology department of various hospitals in Delhi. In-house PCR based assay was developed and modified to visual assay using molecular beacon for end-point detection. It was evaluated against Roche AMPLICOR NG kit and rmp gene. Specificity of beacon was confirmed by competition experiments. Diagnostic test was 98.21% specific and 99.59% sensitive whereas negative and positive predicted value were 99.40% and 98.78%, respectively. We also observed that twice the concentration (2X) of premix was stable at 4°C for 4 months and dry swab samples gave concordant results with that of wet swabs. These features make the test best suitable for routine diagnosis of genital infections in developing countries. PMID:25802857

Measurement of the PPN parameter γ by testing the geometry of near-Earth space

NASA Astrophysics Data System (ADS)

Luo, Jie; Tian, Yuan; Wang, Dian-Hong; Qin, Cheng-Gang; Shao, Cheng-Gang

2016-06-01

The Beyond Einstein Advanced Coherent Optical Network (BEACON) mission was designed to achieve an accuracy of 10^{-9} in measuring the Eddington parameter γ , which is perhaps the most fundamental Parameterized Post-Newtonian parameter. However, this ideal accuracy was just estimated as a ratio of the measurement accuracy of the inter-spacecraft distances to the magnitude of the departure from Euclidean geometry. Based on the BEACON concept, we construct a measurement model to estimate the parameter γ with the least squares method. Influences of the measurement noise and the out-of-plane error on the estimation accuracy are evaluated based on the white noise model. Though the BEACON mission does not require expensive drag-free systems and avoids physical dynamical models of spacecraft, the relatively low accuracy of initial inter-spacecraft distances poses a great challenge, which reduces the estimation accuracy in about two orders of magnitude. Thus the noise requirements may need to be more stringent in the design in order to achieve the target accuracy, which is demonstrated in the work. Considering that, we have given the limits on the power spectral density of both noise sources for the accuracy of 10^{-9}.

A BLE-Based Pedestrian Navigation System for Car Searching in Indoor Parking Garages

PubMed Central

Wang, Sheng-Shih

2018-01-01

The continuous global increase in the number of cars has led to an increase in parking issues, particularly with respect to the search for available parking spaces and finding cars. In this paper, we propose a navigation system for car owners to find their cars in indoor parking garages. The proposed system comprises a car-searching mobile app and a positioning-assisting subsystem. The app guides car owners to their cars based on a “turn-by-turn” navigation strategy, and has the ability to correct the user’s heading orientation. The subsystem uses beacon technology for indoor positioning, supporting self-guidance of the car-searching mobile app. This study also designed a local coordinate system to support the identification of the locations of parking spaces and beacon devices. We used Android as the platform to implement the proposed car-searching mobile app, and used Bytereal HiBeacon devices to implement the proposed positioning-assisting subsystem. We also deployed the system in a parking lot in our campus for testing. The experimental results verified that the proposed system not only works well, but also provides the car owner with the correct route guidance information. PMID:29734753

Secure Utilization of Beacons and UAVs in Emergency Response Systems for Building Fire Hazard.

PubMed

Seo, Seung-Hyun; Choi, Jung-In; Song, Jinseok

2017-09-25

An intelligent emergency system for hazard monitoring and building evacuation is a very important application area in Internet of Things (IoT) technology. Through the use of smart sensors, such a system can provide more vital and reliable information to first-responders and also reduce the incidents of false alarms. Several smart monitoring and warning systems do already exist, though they exhibit key weaknesses such as a limited monitoring coverage and security, which have not yet been sufficiently addressed. In this paper, we propose a monitoring and emergency response method for buildings by utilizing beacons and Unmanned Aerial Vehicles (UAVs) on an IoT security platform. In order to demonstrate the practicability of our method, we also implement a proof of concept prototype, which we call the UAV-EMOR (UAV-assisted Emergency Monitoring and Response) system. Our UAV-EMOR system provides the following novel features: (1) secure communications between UAVs, smart sensors, the control server and a smartphone app for security managers; (2) enhanced coordination between smart sensors and indoor/outdoor UAVs to expand real-time monitoring coverage; and (3) beacon-aided rescue and building evacuation.

Analysis of rocket beacon transmissions for computerized reconstruction of ionospheric densities

NASA Technical Reports Server (NTRS)

Bernhardt, P. A.; Huba, J. D.; Chaturvedi, P. K.; Fulford, J. A.; Forsyth, P. A.; Anderson, D. N.; Zalesak, S. T.

1993-01-01

Three methods are described to obtain ionospheric electron densities from transionospheric, rocket-beacon TEC data. First, when the line-of-sight from a ground receiver to the rocket beacon is tangent to the flight trajectory, the electron concentration can be obtained by differentiating the TEC with respect to the distance to the rocket. A similar method may be used to obtain the electron-density profile if the layer is horizontally stratified. Second, TEC data obtained during chemical release experiments may be interpreted with the aid of physical models of the disturbed ionosphere to yield spatial maps of the modified regions. Third, computerized tomography (CT) can be used to analyze TEC data obtained along a chain of ground-based receivers aligned along the plane of the rocket trajectory. CT analysis of TEC data is used to reconstruct a 2D image of a simulated equatorial plume. TEC data is computed for a linear chain of nine receivers with adjacent spacings of either 100 or 200 km. The simulation data are analyzed to provide an F region reconstruction on a grid with 15 x 15 km pixels. Ionospheric rocket tomography may also be applied to rocket-assisted measurements of amplitude and phase scintillations and airglow intensities.

Method to improve the survival of night-swarming mayflies near bridges in areas of distracting light pollution.

PubMed

Egri, Ádám; Száz, Dénes; Farkas, Alexandra; Pereszlényi, Ádám; Horváth, Gábor; Kriska, György

2017-11-01

Numerous negative ecological effects of urban lighting have been identified during the last decades. In spite of the development of lighting technologies, the detrimental effect of this form of light pollution has not declined. Several insect species are affected including the night-swarming mayfly Ephoron virgo : when encountering bridges during their mass swarming, these mayflies often fall victim to artificial lighting. We show a simple method for the conservation of these mayflies exploiting their positive phototaxis. With downstream-facing light-emitting diode beacon lights above two tributaries of the river Danube, we managed to guide egg-laying females to the water and prevent them from perishing outside the river near urban lights. By means of measuring the mayfly outflow from the river as a function of time and the on/off state of the beacons, we showed that the number of mayflies exiting the river's area was practically zero when our beacons were operating. Tributaries could be the sources of mayfly recolonization in case of water quality degradation of large rivers. The protection of mayfly populations in small rivers and safeguarding their aggregation and oviposition sites is therefore important.

Design of a Combined Beacon Receiver and Digital Radiometer for 40 GHz Propagation Measurements at the Madrid Deep Space Communications Complex

NASA Technical Reports Server (NTRS)

Zemba, Michael; Nessel, James; Morabito, David

2017-01-01

NASA Glenn Research Center (GRC) and the Jet Propulsion Laboratory (JPL) have jointly developed an atmospheric propagation terminal to measure and characterize propagation phenomena at 40 GHz at the Madrid Deep Space Communications Complex (MDSCC) in Robledo de Chavela, Spain. The hybrid Q-band system utilizes a novel design which combines a 40 GHz beacon receiver and digital radiometer into the same RF front-end and observes the 39.402 GHz beacon of the European Space Agencys Alphasat Aldo Paraboni TDP5 experiment. Atmospheric measurements include gaseous absorption, rain fade, and scintillation. The radiometric measurement is calibrated by means of an included noise diode as well as tipping calibration. The goals of these measurements are to assist MDSCC mission operations as the facility increasingly supports Ka-band missions, as well as to contribute to the development and improvement of International Telecommunications Union (ITU) models for prediction of communications systems performance within the Q-band through the Aldo Paraboni Experiment. Herein, we provide an overview of the system design, characterization, and plan of operations which commenced at the MDSCC beginning in March 2017.

May Small Digital PZT And Radio Beacons Improve The LPhL For Future Lunar Missions?

NASA Astrophysics Data System (ADS)

Ping, Jinsong; Su, Xiaoli; Hanada, Hideo; Gusev, Alexandra; Li, Jinling; Shi, Xian; Liu, Qinghui

2012-08-01

LLR is the current unique precise method to measure the LPhL since the Apollo missions. After 40years observations, the measu ring error of LPhL amplitudes have been reduced to about a couple of tens milli-arcseconds. To improve the measuring precision of LPhL, the new ideas of digital PZT (ILOM) and radio beacons are suggested by researchers from Japan, Russia and China for up - coming lunar missions. To promote above ideas in these mission s, we developed a prototype PZT, proposed radio beacons on CE - 3/4 lunar landing missions and on Lunar - Glob/Resource lunar landing missions. We are also developing the small VLBI antennas in Russia and China to prepare the possible LPhL joint in - beam radio observation from later 2013 or earlier 2014. The analyzing work of simulations have been carried out. Additionally, the newly developed digital PZT technique will also be used on measuring the local or regional plume line variation, which has been recently noticed closely related to the volcano and earthquake activities on the Earth (See Li et al. and Yang et al. in this meeting).

Controlling the signal: Practical privacy protection of genomic data sharing through Beacon services.

PubMed

Wan, Zhiyu; Vorobeychik, Yevgeniy; Kantarcioglu, Murat; Malin, Bradley

2017-07-26

Genomic data is increasingly collected by a wide array of organizations. As such, there is a growing demand to make summary information about such collections available more widely. However, over the past decade, a series of investigations have shown that attacks, rooted in statistical inference methods, can be applied to discern the presence of a known individual's DNA sequence in the pool of subjects. Recently, it was shown that the Beacon Project of the Global Alliance for Genomics and Health, a web service for querying about the presence (or absence) of a specific allele, was vulnerable. The Integrating Data for Analysis, Anonymization, and Sharing (iDASH) Center modeled a track in their third Privacy Protection Challenge on how to mitigate the Beacon vulnerability. We developed the winning solution for this track. This paper describes our computational method to optimize the tradeoff between the utility and the privacy of the Beacon service. We generalize the genomic data sharing problem beyond that which was introduced in the iDASH Challenge to be more representative of real world scenarios to allow for a more comprehensive evaluation. We then conduct a sensitivity analysis of our method with respect to several state-of-the-art methods using a dataset of 400,000 positions in Chromosome 10 for 500 individuals from Phase 3 of the 1000 Genomes Project. All methods are evaluated for utility, privacy and efficiency. Our method achieves better performance than all state-of-the-art methods, irrespective of how key factors (e.g., the allele frequency in the population, the size of the pool and utility weights) change from the original parameters of the problem. We further illustrate that it is possible for our method to exhibit subpar performance under special cases of allele query sequences. However, we show our method can be extended to address this issue when the query sequence is fixed and known a priori to the data custodian, so that they may plan stage their responses accordingly. This research shows that it is possible to thwart the attack on Beacon services, without substantially altering the utility of the system, using computational methods. The method we initially developed is limited by the design of the scenario and evaluation protocol for the iDASH Challenge; however, it can be improved by allowing the data custodian to act in a staged manner.

The Southeastern Minnesota Beacon Project for Community-driven Health Information Technology: Origins, Achievements, and Legacy.

PubMed

Chute, Christopher G; Hart, Lacey A; Alexander, Alex K; Jensen, Daniel W

2014-01-01

The Southeastern (SE) Minnesota Beacon organized all the health care providers, county public health organizations, and school districts in the deployment and integration of health information exchange (HIE) and targeted health communication around childhood asthma and diabetes. The community cooperated to establish a clinical data repository for all residents in the 11-county region. Through this community of practice approach that involved traditional and nontraditional providers, the SE Minnesota Beacon was able to realize unique applications of this technology. This manuscript overviews the associated organization and infrastructure of this community collaboration. The Office of the National Coordinator for Health Information Technology (ONC), as part of the American Recovery and Reinvestment Act of 2009 (ARRA) stimulus, established 17 projects throughout the United States targeting the introduction and meaningful use of health information technology (HIT). These 17 communities were intended to serve as an example of what could be accomplished. The SE Minnesota Beacon is one of these communities. The community ultimately opted for peer-to-peer HIE, using Nationwide Health Information Network (NwHIN) Connect software. The clinical data repository was established using the infrastructure developed by the Regenstrief Institute, which operated as a trusted third party. As an extension to HIE, the consortium of county public health departments created a patient data portal for use by school nurses and parents. Childhood asthma was addressed by creating, exchanging, and maintaining an "asthma action plan" for each affected child, shared throughout the community, including through the patient portal. Diabetes management introduced patient treatment decision tools and patient quality of life measures, facilitating care. Influenza vaccination was enhanced by large-scale community reporting in partnership with the state vaccination registry. The methodology and principles for arriving at these solutions included community engagement, sustainability, scalability, standards, and best practices that fit a variety of organizations-from large, robust providers to small organizations. The SE Minnesota Beacon demonstrated that all providers for a geographically defined population can cooperate in the development and shared governance of a low-cost, sustainable HIE, and the operation of a community-managed clinical data repository. Furthermore, these infrastructures can be leveraged to collaboratively improve the care of patients, as demonstrated for childhood asthma and adult diabetes mellitus. The shared governance of HIT by a community can palpably change the scope and success of collaborations targeted to improve patient and community health care.

The International DORIS Service contribution to the 2014 realization of the International Terrestrial Reference Frame

NASA Astrophysics Data System (ADS)

Moreaux, Guilhem; Lemoine, Frank G.; Capdeville, Hugues; Kuzin, Sergey; Otten, Michiel; Štěpánek, Petr; Willis, Pascal; Ferrage, Pascale

2016-12-01

In preparation of the 2014 realization of the International Terrestrial Reference Frame (ITRF2014), the International DORIS Service delivered to the International Earth Rotation and Reference Systems Service a set of 1140 weekly solution files including station coordinates and Earth orientation parameters, covering the time period from 1993.0 to 2015.0. The data come from eleven DORIS satellites: TOPEX/Poseidon, SPOT2, SPOT3, SPOT4, SPOT5, Envisat, Jason-1, Jason-2, Cryosat-2, Saral and HY-2A. In their processing, the six analysis centers which contributed to the DORIS combined solution used the latest time variable gravity models and estimated DORIS ground beacon frequency variations. Furthermore, all the analysis centers but one excepted included in their processing phase center variations for ground antennas. The main objective of this study is to present the combination process and to analyze the impact of the new modeling on the performance of the new combined solution. Comparisons with the IDS contribution to ITRF2008 show that (i) the application of the DORIS ground phase center variations in the data processing shifts the combined scale upward by nearly 7-11 mm and (ii) thanks to estimation of DORIS ground beacon frequency variations, the new combined solution no longer shows any scale discontinuity in early 2002 and does not present unexplained vertical discontinuities in any station position time series. However, analysis of the new series with respect to ITRF2008 exhibits a scale increase late 2011 which is not yet explained. A new DORIS Terrestrial Reference Frame was computed to evaluate the intrinsic quality of the new combined solution. That evaluation shows that the addition of data from the new missions equipped with the latest generation of DORIS receiver (Jason-2, Cryosat-2, HY-2A, Saral), results in an internal position consistency of 10 mm or better after mid-2008.

Racial Differences in Processes of Care at End of Life in VA Medical Centers: Planned Secondary Analysis of Data from the BEACON Trial

PubMed Central

Williams, Beverly R.; Dionne-Odom, J. Nicholas; Redden, David T.; Noh, Hyunjin; Goode, Patricia S.; Kvale, Elizabeth; Bakitas, Marie; Bailey, F. Amos

2016-01-01

Abstract Background: Racial differences exist for a number of health conditions, services, and outcomes, including end-of-life (EOL) care. Objective: The aim of the study was to examine differences in processes of care in the last 7 days of life between African American and white inpatients. Methods: Secondary analysis was conducted of data collected in the Best Practices for End-of-Life Care for Our Nation's Veterans (BEACON) trial (conducted 2005–2011). Subjects were 4891 inpatient decedents in six Veterans Administration Medical Centers. Data were abstracted from decedents' medical records. Multi-variable analyses were conducted to examine the relationship between race and each of 18 EOL processes of care controlling for patient characteristics, study site, year of death, and whether the observation was pre- or post-intervention. Results: The sample consisted of 1690 African American patients (34.6%) and 3201 white patients (65.4%). African Americans were less likely to have: do not resuscitate (DNR) orders (odds ratio [OR]: 0.67; p = 0.004), advance directives (OR: 0.71; p = 0.023), active opioid orders (OR: 0.64, p = 0.0008), opioid medications administered (OR: 0.61, p = 0.004), benzodiazepine orders (OR: 0.68, p < 0.0001), benzodiazepines administered (OR: 0.61, p < 0.0001), antipsychotics administered (OR: 0.73, p = 0.004), and steroids administered (OR: 0.76, p = 0.020). Racial differences were not found for other processes of care, including palliative care consultation, pastoral care, antipsychotic and steroid orders, and location of death. Conclusions: Racial differences exist in some but not all aspects of EOL care. Further study is needed to understand the extent to which racial differences reflect different patient needs and preferences and whether interventions are needed to reduce disparities in patient/family education or access to quality EOL care. PMID:26840851

Racial Differences in Processes of Care at End of Life in VA Medical Centers: Planned Secondary Analysis of Data from the BEACON Trial.

PubMed

Burgio, Kathryn L; Williams, Beverly R; Dionne-Odom, J Nicholas; Redden, David T; Noh, Hyunjin; Goode, Patricia S; Kvale, Elizabeth; Bakitas, Marie; Bailey, F Amos

2016-02-01

Racial differences exist for a number of health conditions, services, and outcomes, including end-of-life (EOL) care. The aim of the study was to examine differences in processes of care in the last 7 days of life between African American and white inpatients. Secondary analysis was conducted of data collected in the Best Practices for End-of-Life Care for Our Nation's Veterans (BEACON) trial (conducted 2005-2011). Subjects were 4891 inpatient decedents in six Veterans Administration Medical Centers. Data were abstracted from decedents' medical records. Multi-variable analyses were conducted to examine the relationship between race and each of 18 EOL processes of care controlling for patient characteristics, study site, year of death, and whether the observation was pre- or post-intervention. The sample consisted of 1690 African American patients (34.6%) and 3201 white patients (65.4%). African Americans were less likely to have: do not resuscitate (DNR) orders (odds ratio [OR]: 0.67; p = 0.004), advance directives (OR: 0.71; p = 0.023), active opioid orders (OR: 0.64, p = 0.0008), opioid medications administered (OR: 0.61, p = 0.004), benzodiazepine orders (OR: 0.68, p < 0.0001), benzodiazepines administered (OR: 0.61, p < 0.0001), antipsychotics administered (OR: 0.73, p = 0.004), and steroids administered (OR: 0.76, p = 0.020). Racial differences were not found for other processes of care, including palliative care consultation, pastoral care, antipsychotic and steroid orders, and location of death. Racial differences exist in some but not all aspects of EOL care. Further study is needed to understand the extent to which racial differences reflect different patient needs and preferences and whether interventions are needed to reduce disparities in patient/family education or access to quality EOL care.

The Argos contribution to the demonstration of the effectiveness of a satellite-based search and rescue system

NASA Astrophysics Data System (ADS)

Rolland, R.

The use of the CNES/NASA/NOAA Argos environmental-research instrumentation on the Tiros-N series of satellites as a search and rescue location-finding system for trans-Atlantic yacht races during 1979-1982 is described. The transmission beacons, satellite equipment, data-processing center, and data distribution facilities of Argos are characterized and illustrated; the nine race rescue operations in which Argos was involved are listed and discussed; and the deficiencies of Argos are shown to be fully corrected in the 406-MHz location system developed for Sarsat.

Facile conversion of ATP-binding RNA aptamer to quencher-free molecular aptamer beacon.

PubMed

Park, Yoojin; Nim-Anussornkul, Duangrat; Vilaivan, Tirayut; Morii, Takashi; Kim, Byeang Hyean

2018-01-15

We have developed RNA-based quencher-free molecular aptamer beacons (RNA-based QF-MABs) for the detection of ATP, taking advantage of the conformational changes associated with ATP binding to the ATP-binding RNA aptamer. The RNA aptamer, with its well-defined structure, was readily converted to the fluorescence sensors by incorporating a fluorophore into the loop region of the hairpin structure. These RNA-based QF-MABs exhibited fluorescence signals in the presence of ATP relative to their low background signals in the absence of ATP. The fluorescence emission intensity increased upon formation of a RNA-based QF-MAB·ATP complex. Copyright © 2017 Elsevier Ltd. All rights reserved.

Low Cost and Flexible UAV Deployment of Sensors

PubMed Central

Sørensen, Lars Yndal; Jacobsen, Lars Toft; Hansen, John Paulin

2017-01-01

This paper presents a platform for airborne sensor applications using low-cost, open-source components carried by an easy-to-fly unmanned aircraft vehicle (UAV). The system, available in open-source , is designed for researchers, students and makers for a broad range of exploration and data-collection needs. The main contribution is the extensible architecture for modularized airborne sensor deployment and real-time data visualisation. Our open-source Android application provides data collection, flight path definition and map tools. Total cost of the system is below 800 dollars. The flexibility of the system is illustrated by mapping the location of Bluetooth beacons (iBeacons) on a ground field and by measuring water temperature in a lake. PMID:28098819

Low Cost and Flexible UAV Deployment of Sensors.

PubMed

Sørensen, Lars Yndal; Jacobsen, Lars Toft; Hansen, John Paulin

2017-01-14

This paper presents a platform for airborne sensor applications using low-cost, open-source components carried by an easy-to-fly unmanned aircraft vehicle (UAV). The system, available in open-source , is designed for researchers, students and makers for a broad range of exploration and data-collection needs. The main contribution is the extensible architecture for modularized airborne sensor deployment and real-time data visualisation. Our open-source Android application provides data collection, flight path definition and map tools. Total cost of the system is below 800 dollars. The flexibility of the system is illustrated by mapping the location of Bluetooth beacons (iBeacons) on a ground field and by measuring water temperature in a lake.

Burial and exhumation of the Eisenhower Range, Transantarctic Mountains, based on thermochronological, sedimentary rock maturity and petrographic constraints

NASA Astrophysics Data System (ADS)

Prenzel, J.; Lisker, F.; Elsner, M.; Schöner, R.; Balestrieri, M. L.; Läufer, A. L.; Berner, U.; Spiegel, C.

2014-09-01

The Eisenhower Range is a N-S trending mountain range in the Transantarctic Mountains (TAM) adjacent to the NW Ross Sea Embayment. New AFT and apatite (U-Th-Sm)/He (AHe) data from vertical basement profiles supplemented by paleotemperature and pressure estimates derived from Beacon sandstones provide new quantitative results on regional burial evolution and first regional constraints on basin inversion and exhumation processes. AFT ages between 32 ± 2 and 259 ± 18 Ma and AHe ages of 37 ± 3-173 ± 16 Ma correlate positively with sample elevations. Thermal history modeling of these data and complementary thermal indications detect heating of the paleosurface on the Eisenhower Range to temperatures ≥ 80 °C subsequent to Ferrar magmatism, and constrain Late Eocene rapid cooling. Regression of modeled paleotemperatures against sample elevations refers to a high Jurassic (~ 45 °C/km) and a moderate Cretaceous-Eocene (28 ± 8 °C/km) geothermal gradient. The texture of Beacon sandstones supports strong mechanical compaction that requires a higher overburden than preserved in the stratigraphic record. Modeled paleotemperatures and pressures suggest basement burial that increases from Late Jurassic (0.7-1.1 km) to Eocene (1.8-2.1 km). The overburden comprises 0.7-1.1 km cumulative Beacon/Ferrar rocks and 0.7-1.4 km of post-Ferrar sediments. Rapid cooling of the whole sample suite between ~ 35 and 30 Ma implies fast erosion of the post-Ferrar sediments and (re-) exposure of underlying magmatic rocks. Subsequent differential sample cooling to present-day surface temperature infers ongoing exhumation by glacial incision enhanced by isostatic response to basin inversion. Decreasing amounts of exhumation from the coast (> 3 km) toward the interior (1.5-2.2 km) point to backstepping incision along the fault controlled Priestley Glacier. Substantial exhumation of the Eisenhower Range since the Late Eocene is hence triggered by both tectonic and climatic factors, superimposed by considerable lithological influence during the initial exhumation stage.

Quantum Dot-Fullerene Based Molecular Beacon Nanosensors for Rapid, Highly Sensitive Nucleic Acid Detection.

PubMed

Liu, Ye; Kannegulla, Akash; Wu, Bo; Cheng, Li-Jing

2018-05-15

Spherical fullerene (C 60 ) can quench the fluorescence of a quantum dot (QD) through energy transfer and charge transfer processes, with the quenching efficiency regulated by the number of proximate C 60 on each QD. With the quenching property and its small size compared with other nanoparticle-based quenchers, it is advantageous to group a QD reporter and multiple C 60 -labeled oligonucleotide probes to construct a molecular beacon (MB) probe for sensitive, robust nucleic acid detection. We demonstrated a rapid, high-sensitivity DNA detection method using the nanosensors composed of QD-C 60 based MBs carried by magnetic nanoparticles (MNPs). The assay was accelerated by first dispersing the nanosensors in analytes for highly efficient DNA capture resulting from short-distance 3-dimensional diffusion of targets to the sensor surface and then concentrating the nanosensors to a substrate by magnetic force to amplify the fluorescence signal for target quantification. The enhanced mass transport enabled a rapid detection (< 10 min) with a small sample volume (1-10 µl). The high signal-to-noise ratio produced by the QD-C 60 pairs and magnetic concentration yielded a detection limit of 100 fM (~106 target DNA copies for a 10 µl analyte). The rapid, sensitive, label-free detection method will benefit the applications in point-of-care molecular diagnostic technologies.

Noninvasive Monitoring of Three-Dimensional Chondrogenic Constructs Using Molecular Beacon Nanosensors.

PubMed

Tay, Li Min; Wiraja, Christian; Yeo, David C; Wu, Yingnan; Yang, Zheng; Chuah, Yon Jin; Lee, Eng Hin; Kang, Yuejun; Xu, Chenjie

2017-01-01

Chondrogenic differentiation of human mesenchymal stem cells (MSCs) in three-dimensional hydrogel holds promise as a method for repairing injured articular cartilage. Given MSC plasticity (its potential to mature into alternative lineages), nondestructive monitoring is critical for the optimization of chondrogenic differentiation conditions and the evaluation of the final product. However, conventional validation/assessments of the differentiation process (i.e., quantitative reverse transcription polymerase chain reaction [qRT-PCR] and histology) are end-point assays requiring disruption of the sample. This report introduces molecular beacon (MB)-based nanosensors to achieve noninvasive monitoring of chondrogenic differentiation. These nanosensors consist of biodegradable poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) encapsulating MBs to detect Type II Collagen (Col2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNAs that serve as lineage-specific and housekeeping biomarkers, respectively. The sustainable release of MBs from MB-NPs allows longitudinal monitoring of MSCs undergoing chondrogenic differentiation over a period of 28 days. Dual-colored MB loading ensures accurate assessment of Col2 mRNA expression level, where potential heterogeneity in nanosensor uptake and retention by MSCs are taken into account. When normalized nanosensor signal was compared against qRT-PCR result, a tight correlation was observed (R 2  = 0.9301). Finally, nanosensor usage was compatible with MSC potency with minimal influence on chondrogenic, adipogenic, and osteogenic differentiation.

Solid-Phase Nucleic Acid Sequence-Based Amplification and Length-Scale Effects during RNA Amplification.

PubMed

Ma, Youlong; Teng, Feiyue; Libera, Matthew

2018-06-05

Solid-phase oligonucleotide amplification is of interest because of possible applications to next-generation sequencing, multiplexed microarray-based detection, and cell-free synthetic biology. Its efficiency is, however, less than that of traditional liquid-phase amplification involving unconstrained primers and enzymes, and understanding how to optimize the solid-phase amplification process remains challenging. Here, we demonstrate the concept of solid-phase nucleic acid sequence-based amplification (SP-NASBA) and use it to study the effect of tethering density on amplification efficiency. SP-NASBA involves two enzymes, avian myeloblastosis virus reverse transcriptase (AMV-RT) and RNase H, to convert tethered forward and reverse primers into tethered double-stranded DNA (ds-DNA) bridges from which RNA - amplicons can be generated by a third enzyme, T7 RNA polymerase. We create microgels on silicon surfaces using electron-beam patterning of thin-film blends of hydroxyl-terminated and biotin-terminated poly(ethylene glycol) (PEG-OH, PEG-B). The tethering density is linearly related to the PEG-B concentration, and biotinylated primers and molecular beacon detection probes are tethered to streptavidin-activated microgels. While SP-NASBA is very efficient at low tethering densities, the efficiency decreases dramatically with increasing tethering density due to three effects: (a) a reduced hybridization efficiency of tethered molecular beacon detection probes; (b) a decrease in T7 RNA polymerase efficiency; (c) inhibition of T7 RNA polymerase activity by AMV-RT.

Detection of Ochratoxin a Using Molecular Beacons and Real-Time PCR Thermal Cycler

PubMed Central

Sanzani, Simona Marianna; Reverberi, Massimo; Fanelli, Corrado; Ippolito, Antonio

2015-01-01

We developed a simple and cheap assay for quantitatively detecting ochratoxin A (OTA) in wine. A DNA aptamer available in literature was used as recognition probe in its molecular beacon form, i.e., with a fluorescence-quenching pair at the stem ends. Our aptabeacon could adopt a conformation allowing OTA binding, causing a fluorescence rise due to the increased distance between fluorophore and quencher. We used real-time PCR equipment for capturing the signal. With this assay, under optimized conditions, the entire process can be completed within 1 h. In addition, the proposed system exhibited a good selectivity for OTA against other mycotoxins (ochratoxin B and aflatoxin M1) and limited interference from aflatoxin B1 and patulin. A wide linear detection range (0.2–2000 µM) was achieved, with LOD = 13 nM, r = 0.9952, and R2 = 0.9904. The aptabeacon was also applied to detect OTA in red wine spiked with the same dilution series. A linear correlation with a LOD = 19 nM, r = 0.9843, and R2 = 0.9708 was observed, with recoveries in the range 63%–105%. Intra- and inter-day assays confirmed its reproducibility. The proposed biosensor, although still being finalized, might significantly facilitate the quantitative detection of OTA in wine samples, thus improving their quality control from a food safety perspective. PMID:25760080

Transfer of Real-time Dynamic Radiation Environment Assimilation Model; Research to Operation

NASA Astrophysics Data System (ADS)

Cho, K. S. F.; Hwang, J.; Shin, D. K.; Kim, G. J.; Morley, S.; Henderson, M. G.; Friedel, R. H.; Reeves, G. D.

2015-12-01

Real-time Dynamic Radiation Environment Assimilation Model (rtDREAM) was developed by LANL for nowcast of energetic electrons' flux at the radiation belt to quantify potential risks from radiation damage at the satellites. Assimilated data are from multiple sources including LANL assets (GEO, GPS). For transfer from research to operation of the rtDREAM code, LANL/KSWC/NOAA makes a Memorandum Of Understanding (MOU) on the collaboration between three parts. By this MOU, KWSC/RRA provides all the support for transitioning the research version of DREAM to operations. KASI is primarily responsible for providing all the interfaces between the current scientific output formats of the code and useful space weather products that can be used and accessed through the web. In the second phase, KASI will be responsible in performing the work needed to transform the Van Allen Probes beacon data into "DREAM ready" inputs. KASI will also provide the "operational" code framework and additional data preparation, model output, display and web page codes back to LANL and SWPC. KASI is already a NASA partnering ground station for the Van Allen Probes' space weather beacon data and can here show use and utility of these data for comparison between rtDREAM and observations by web. NOAA has offered to take on some of the data processing tasks specific to the GOES data.

Temporal change of EIA asymmetry revealed by a beacon receiver network in Southeast Asia

NASA Astrophysics Data System (ADS)

Watthanasangmechai, Kornyanat; Yamamoto, Mamoru; Saito, Akinori; Maruyama, Takashi; Yokoyama, Tatsuhiro; Nishioka, Michi; Ishii, Mamoru

2015-05-01

To reveal the temporal change of the equatorial ionization anomaly (EIA) asymmetry, a multipoint satellite-ground beacon experiment was conducted along the meridional plane of the Thailand-Indonesia sector. The observation includes one station near the magnetic equator and four stations at off-equator latitudes. This is the first EIA asymmetry study with high spatial resolution using GNU Radio Beacon Receiver (GRBR) observations in Southeast Asia. GRBR-total electron contents (TECs) from 97 polar-orbit satellite passes in March 2012 were analyzed in this study. Successive passes captured rapid evolution of EIA asymmetry, especially during geomagnetic disturbances. The penetrating electric fields that occur during geomagnetic disturbed days are not the cause of the asymmetry. Instead, high background TEC associated with an intense electric field empowers the neutral wind to produce severe asymmetry of the EIA. Such rapid evolution of EIA asymmetry was not seen during nighttime, when meridional wind mainly controlled the asymmetric structures. Additional data are necessary to identify the source of the variations, i.e., atmospheric waves. Precisely capturing the locations of the crests and the evolution of the asymmetry enhances understanding of the temporal change of EIA asymmetry at the local scale and leads to a future local modeling for TEC prediction in Southeast Asia.

First results from the ionospheric tomography experiment using beacon TEC data obtained by means of a network along a longitude of 136°E over Japan

NASA Astrophysics Data System (ADS)

Thampi, Smitha V.; Yamamoto, Mamoru

2010-03-01

A chain of newly designed GNU (GNU is not UNIX) Radio Beacon Receivers (GRBR) has recently been established over Japan, primarily for tomographic imaging of the ionosphere over this region. Receivers installed at Shionomisaki (33.45°N, 135.8°E), Shigaraki (34.8°N, 136.1°E), and Fukui (36°N, 136°E) continuously track low earth orbiting satellites (LEOS), mainly OSCAR, Cosmos, and FORMOSAT-3/COSMIC, to obtain simultaneous total electron content (TEC) data from these three locations, which are then used for the tomographic reconstruction of ionospheric electron densities. This is the first GRBR network established for TEC observations, and the first beacon-based tomographic imaging in Japanese longitudes. The first tomographic images revealed the temporal evolution with all of the major features in the ionospheric electron density distribution over Japan. A comparison of the tomographically reconstructed electron densities with the ƒ o F 2 data from Kokubunji (35°N, 139°E) revealed that there was good agreement between the datasets. These first results show the potential of GRBR and its network for making continuous, unattended ionospheric TEC measurements and for tomographic imaging of the ionosphere.

An Empirical Study of the Transmission Power Setting for Bluetooth-Based Indoor Localization Mechanisms

PubMed Central

Castillo-Cara, Manuel; Lovón-Melgarejo, Jesús; Bravo-Rocca, Gusseppe; Orozco-Barbosa, Luis; García-Varea, Ismael

2017-01-01

Nowadays, there is a great interest in developing accurate wireless indoor localization mechanisms enabling the implementation of many consumer-oriented services. Among the many proposals, wireless indoor localization mechanisms based on the Received Signal Strength Indication (RSSI) are being widely explored. Most studies have focused on the evaluation of the capabilities of different mobile device brands and wireless network technologies. Furthermore, different parameters and algorithms have been proposed as a means of improving the accuracy of wireless-based localization mechanisms. In this paper, we focus on the tuning of the RSSI fingerprint to be used in the implementation of a Bluetooth Low Energy 4.0 (BLE4.0) Bluetooth localization mechanism. Following a holistic approach, we start by assessing the capabilities of two Bluetooth sensor/receiver devices. We then evaluate the relevance of the RSSI fingerprint reported by each BLE4.0 beacon operating at various transmission power levels using feature selection techniques. Based on our findings, we use two classification algorithms in order to improve the setting of the transmission power levels of each of the BLE4.0 beacons. Our main findings show that our proposal can greatly improve the localization accuracy by setting a custom transmission power level for each BLE4.0 beacon. PMID:28590413

An Empirical Study of the Transmission Power Setting for Bluetooth-Based Indoor Localization Mechanisms.

PubMed

Castillo-Cara, Manuel; Lovón-Melgarejo, Jesús; Bravo-Rocca, Gusseppe; Orozco-Barbosa, Luis; García-Varea, Ismael

2017-06-07

Nowadays, there is a great interest in developing accurate wireless indoor localization mechanisms enabling the implementation of many consumer-oriented services. Among the many proposals, wireless indoor localization mechanisms based on the Received Signal Strength Indication (RSSI) are being widely explored. Most studies have focused on the evaluation of the capabilities of different mobile device brands and wireless network technologies. Furthermore, different parameters and algorithms have been proposed as a means of improving the accuracy of wireless-based localization mechanisms. In this paper, we focus on the tuning of the RSSI fingerprint to be used in the implementation of a Bluetooth Low Energy 4.0 (BLE4.0) Bluetooth localization mechanism. Following a holistic approach, we start by assessing the capabilities of two Bluetooth sensor/receiver devices. We then evaluate the relevance of the RSSI fingerprint reported by each BLE4.0 beacon operating at various transmission power levels using feature selection techniques. Based on our findings, we use two classification algorithms in order to improve the setting of the transmission power levels of each of the BLE4.0 beacons. Our main findings show that our proposal can greatly improve the localization accuracy by setting a custom transmission power level for each BLE4.0 beacon.

Parametric study of sensor placement for vision-based relative navigation system of multiple spacecraft

NASA Astrophysics Data System (ADS)

Jeong, Junho; Kim, Seungkeun; Suk, Jinyoung

2017-12-01

In order to overcome the limited range of GPS-based techniques, vision-based relative navigation methods have recently emerged as alternative approaches for a high Earth orbit (HEO) or deep space missions. Therefore, various vision-based relative navigation systems use for proximity operations between two spacecraft. For the implementation of these systems, a sensor placement problem can occur on the exterior of spacecraft due to its limited space. To deal with the sensor placement, this paper proposes a novel methodology for a vision-based relative navigation based on multiple position sensitive diode (PSD) sensors and multiple infrared beacon modules. For the proposed method, an iterated parametric study is used based on the farthest point optimization (FPO) and a constrained extended Kalman filter (CEKF). Each algorithm is applied to set the location of the sensors and to estimate relative positions and attitudes according to each combination by the PSDs and beacons. After that, scores for the sensor placement are calculated with respect to parameters: the number of the PSDs, number of the beacons, and accuracy of relative estimates. Then, the best scoring candidate is determined for the sensor placement. Moreover, the results of the iterated estimation show that the accuracy improves dramatically, as the number of the PSDs increases from one to three.

Analysis of SSEM Sensor Data Using BEAM

NASA Technical Reports Server (NTRS)

Zak, Michail; Park, Han; James, Mark

2004-01-01

A report describes analysis of space shuttle main engine (SSME) sensor data using Beacon-based Exception Analysis for Multimissions (BEAM) [NASA Tech Briefs articles, the two most relevant being Beacon-Based Exception Analysis for Multimissions (NPO- 20827), Vol. 26, No.9 (September 2002), page 32 and Integrated Formulation of Beacon-Based Exception Analysis for Multimissions (NPO- 21126), Vol. 27, No. 3 (March 2003), page 74] for automated detection of anomalies. A specific implementation of BEAM, using the Dynamical Invariant Anomaly Detector (DIAD), is used to find anomalies commonly encountered during SSME ground test firings. The DIAD detects anomalies by computing coefficients of an autoregressive model and comparing them to expected values extracted from previous training data. The DIAD was trained using nominal SSME test-firing data. DIAD detected all the major anomalies including blade failures, frozen sense lines, and deactivated sensors. The DIAD was particularly sensitive to anomalies caused by faulty sensors and unexpected transients. The system offers a way to reduce SSME analysis time and cost by automatically indicating specific time periods, signals, and features contributing to each anomaly. The software described here executes on a standard workstation and delivers analyses in seconds, a computing time comparable to or faster than the test duration itself, offering potential for real-time analysis.

Screening mTOR siRNA based on bioinformatics and detecting the transcription by the gold nanoparticle beacon

NASA Astrophysics Data System (ADS)

Tian, Caiping; Ma, Yi; Li, Siwen; Gu, Yueqing

2014-09-01

Mammalian target of rapamycin (mTOR) as a key protein in PI3K-AKT-mTOR signaling pathway ,plays an important role in the tumor growth. The small interfering RNA (siRNA) of mTOR would decrease the expression of mTOR protein. In this study, we screened the mTOR siRNA sequence using MATLAB software and ascertained it based on BLAST. Then we imported it with the aid of Lipofectamine2000 into MCF-7 cancer cells where mTOR is over expression .And then we used a special hairpin deoxyribonucleic acid (DNA) for combining with the human mTOR mRNA to functionalize gold nanoparticles, which served as a molecule beacon for detecting human mTOR mRNA transcription. Laser scanning confocal microscope and Flow Cytometry data showed that the quenching efficiency was up to 90%,which are consistent with the RT-PCR measurement and Western. Compared to the previous approaches, this beacon has advantages of higher target to background ratio of detection. The strategy reported in this study is a promising approach for the intracellular measurement of the result of siRNA or protein expression in living cells, and has great potential in the study of drug screening and discovery.

SU-E-T-574: Fessiblity of Using the Calypso System for HDR Interstitial Catheter Reconstruction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, J S; Ma, C

2014-06-01

Purpose: It is always a challenge to reconstruct the interstitial catheter for high dose rate (HDR) brachytherapy on patient CT or MR images. This work aims to investigate the feasibility of using the Calypso system (Varian Medical, CA) for HDR catheter reconstruction utilizing its accuracy on tracking the electromagnetic transponder location. Methods: Experiment was done with a phantom that has a HDR interstitial catheter embedded inside. CT scan with a slice thickness of 1.25 mm was taken for this phantom with two Calypso beacon transponders in the catheter. The two transponders were connected with a wire. The Calypso system wasmore » used to record the beacon transponders’ location in real time when they were gently pulled out with the wire. The initial locations of the beacon transponders were used for registration with the CT image and the detected transponder locations were used for the catheter path reconstruction. The reconstructed catheter path was validated on the CT image. Results: The HDR interstitial catheter was successfully reconstructed based on the transponders’ coordinates recorded by the Calypso system in real time when the transponders were pulled in the catheter. After registration with the CT image, the shape and location of the reconstructed catheter are evaluated against the CT image and the result shows an accuracy of 2 mm anywhere in the Calypso detectable region which is within a 10 cm X 10 cm X 10 cm cubic box for the current system. Conclusion: It is feasible to use the Calypso system for HDR interstitial catheter reconstruction. The obstacle for its clinical usage is the size of the beacon transponder whose diameter is bigger than most of the interstitial catheters used in clinic. Developing smaller transponders and supporting software and hardware for this application is necessary before it can be adopted for clinical use.« less

SU-G-JeP1-01: A Combination of Real Time Electromagnetic Localization and Tracking with Cone Beam Computed Tomography in Stereotactic Radiosurgery for Brain Tumors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Muralidhar, K Raja; Pangam, Suresh; Ponaganti, Srinivas

2016-06-15

Purpose: 1. online verification of patient position during treatment using calypso electromagnetic localization and tracking system. 2. Verification and comparison of positional accuracy between cone beam computed tomography and calypso system. 3. Presenting the advantage of continuation localization in Stereotactic radiosurgery treatments. Methods: Ten brain tumor cases were taken for this study. Patients with head mask were under gone Computed Tomography (CT). Before scanning, mask was cut on the fore head area to keep surface beacons on the skin. Slice thickness of 0.65 mm were taken for this study. x, y, z coordinates of these beacons in TPS were enteredmore » into tracking station. Varian True Beam accelerator, equipped with On Board Imager was used to take Cone beam Computed Tomography (CBCT) to localize the patient. Simultaneously Surface beacons were used to localize and track the patient throughout the treatment. The localization values were compared in both systems. For localization CBCT considered as reference. Tracking was done throughout the treatment using Calypso tracking system using electromagnetic array. This array was in tracking position during imaging and treatment. Flattening Filter free beams of 6MV photons along with Volumetric Modulated Arc Therapy was used for the treatment. The patient movement was observed throughout the treatment ranging from 2 min to 4 min. Results: The average variation observed between calypso system and CBCT localization was less than 0.5 mm. These variations were due to manual errors while keeping beacon on the patient. Less than 0.05 cm intra-fraction motion was observed throughout the treatment with the help of continuous tracking. Conclusion: Calypso target localization system is one of the finest tools to perform radiosurgery in combination with CBCT. This non radiographic method of tracking is a real beneficial method to treat patients confidently while observing real-time motion information of the patient.« less

Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules.

PubMed

Mohamad, Nurhidayatul Asma; Mustafa, Shuhaimi; Khairil Mokhtar, Nur Fadhilah; El Sheikha, Aly Farag

2018-03-05

The pharmaceutical industry has boosted gelatin consumption worldwide. This is supported by the availability of cost-effective gelatin production from porcine by-products. However, cross-contamination of gelatin materials, where porcine gelatin was unintentionally included in the other animal sources of gelatin, has caused significant concerns about halal authenticity. The real-time polymerase chain reaction (PCR) has enabled a highly specific and sensitive animal species detection method in various food products. Hence, such a technique was employed in the present study to detect and quantify porcine DNA in gelatin using a molecular beacon probe, with differences in performance between mitochondrial (cytochrome b gene) and chromosomal DNA-(MPRE42 repetitive element) based porcine-specific PCR assays being compared. A higher sensitivity was observed in chromosomal DNA (MPRE-PCR assay), where this assay allows the detection of gelatin DNA at amounts as as low as 1 pg, whereas mitochondrial DNA (CBH-PCR assay) can only detect at levels down to 10 pg of gelatin DNA. When an analysis with commercial gelatin and gelatin capsule samples was conducted, the same result was observed, with a significantly more sensitive detection being provided by the repetitive element of chromosomal DNA. The present study has established highly sensitive DNA-based porcine detection systems derived from chromosomal DNA that are feasible for highly processed products such as gelatin and gelatin capsules containing a minute amount of DNA. This sensitive detection method can also be implemented to assist the halal authentication process of various food products available on the market. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

HamSCI: The Ham Radio Science Citizen Investigation

NASA Astrophysics Data System (ADS)

Frissell, N. A.; Moses, M. L.; Earle, G. D.; McGwier, R. W.; Miller, E. S.; Kaeppler, S. R.; Silver, H. W.; Ceglia, F.; Pascoe, D.; Sinanis, N.; Smith, P.; Williams, R.; Shovkoplyas, A.; Gerrard, A. J.

2016-12-01

Amateur (or "ham") radio operators are individuals with a non-pecuniary interest in radio technology, engineering, communications, science, and public service. They are licensed by their national governments to transmit on amateur radio frequencies. In many jurisdictions, there is no age requirement for a ham radio license, and operators from diverse backgrounds participate. There are more than 740,000 hams in the US, and over 3 million (estimated) worldwide. Many amateur communications are conducted using transionospheric links and thus affected by space weather and ionospheric processes. Recent technological advances have enabled the development of automated ham radio observation networks (e.g. the Reverse Beacon Network, www.reversebeacon.net) and specialized operating modes for the study of weak-signal propagation. The data from these networks have been shown to be useful for the study of ionospheric processes. In order to connect professional researchers with the volunteer-based ham radio community, HamSCI (Ham Radio Science Citizen Investigation, www.hamsci.org) has been established. HamSCI is a platform for publicizing and promoting projects that are consistent with the following objectives: (1) Advance scientific research and understanding through amateur radio activities. (2) Encourage the development of new technologies to support this research. (3) Provide educational opportunities for the amateur community and the general public. HamSCI researchers are working with the American Radio Relay League (ARRL, www.arrl.org) to publicize these objectives and recruit interested hams. The ARRL is the US national organization for amateur radio with a membership of over 170,000 and a monthly magazine, QST. HamSCI is currently preparing to support ionospheric research connected to the 21 Aug 2017 Total Solar Eclipse by expanding coverage of the Reverse Beacon Network and organizing a large-scale ham radio operating event ("QSO Party") to generate data during the eclipse.

The Southeastern Minnesota Beacon Project for Community-driven Health Information Technology: Origins, Achievements, and Legacy

PubMed Central

Chute, Christopher G.; Hart, Lacey A.; Alexander, Alex K.; Jensen, Daniel W.

2014-01-01

Introduction: The Southeastern (SE) Minnesota Beacon organized all the health care providers, county public health organizations, and school districts in the deployment and integration of health information exchange (HIE) and targeted health communication around childhood asthma and diabetes. The community cooperated to establish a clinical data repository for all residents in the 11-county region. Through this community of practice approach that involved traditional and nontraditional providers, the SE Minnesota Beacon was able to realize unique applications of this technology. This manuscript overviews the associated organization and infrastructure of this community collaboration. Background: The Office of the National Coordinator for Health Information Technology (ONC), as part of the American Recovery and Reinvestment Act of 2009 (ARRA) stimulus, established 17 projects throughout the United States targeting the introduction and meaningful use of health information technology (HIT). These 17 communities were intended to serve as an example of what could be accomplished. The SE Minnesota Beacon is one of these communities. Methods: The community ultimately opted for peer-to-peer HIE, using Nationwide Health Information Network (NwHIN) Connect software. The clinical data repository was established using the infrastructure developed by the Regenstrief Institute, which operated as a trusted third party. As an extension to HIE, the consortium of county public health departments created a patient data portal for use by school nurses and parents. Childhood asthma was addressed by creating, exchanging, and maintaining an “asthma action plan” for each affected child, shared throughout the community, including through the patient portal. Diabetes management introduced patient treatment decision tools and patient quality of life measures, facilitating care. Influenza vaccination was enhanced by large-scale community reporting in partnership with the state vaccination registry. The methodology and principles for arriving at these solutions included community engagement, sustainability, scalability, standards, and best practices that fit a variety of organizations—from large, robust providers to small organizations. Findings: The SE Minnesota Beacon demonstrated that all providers for a geographically defined population can cooperate in the development and shared governance of a low-cost, sustainable HIE, and the operation of a community-managed clinical data repository. Furthermore, these infrastructures can be leveraged to collaboratively improve the care of patients, as demonstrated for childhood asthma and adult diabetes mellitus. Conclusion: The shared governance of HIT by a community can palpably change the scope and success of collaborations targeted to improve patient and community health care. PMID:25848625

50 CFR 600.746 - Observers.

Code of Federal Regulations, 2012 CFR

2012-10-01

... USCG regulations: (1) Personal flotation devices/ immersion suits; (2) Ring buoys; (3) Distress signals; (4) Fire extinguishing equipment; (5) Emergency position indicating radio beacon (EPIRB), when...

50 CFR 600.746 - Observers.

Code of Federal Regulations, 2014 CFR

2014-10-01

... USCG regulations: (1) Personal flotation devices/ immersion suits; (2) Ring buoys; (3) Distress signals; (4) Fire extinguishing equipment; (5) Emergency position indicating radio beacon (EPIRB), when...

Real-time monitoring of disintegration activity of catalytic core domain of HIV-1 integrase using molecular beacon.

PubMed

Zhang, Da-wei; Zhao, Ming-ming; He, Hong-qiu; Guo, Shun-xing

2013-09-15

HIV-1 integrase, an essential enzyme for retroviral replication, is a validated target for anti-HIV therapy development. The catalytic core domain of integrase (IN-CCD) is capable of catalyzing disintegration reaction. In this work, a hairpin-shaped disintegration substrate was designed and validated by enzyme-linked immunosorbent assay; a molecular beacon-based assay was developed for disintegration reaction of IN-CCD. Results showed that the disintegration substrate could be recognized and catalyzed by IN-CCD, and the disintegration reaction can be monitored according to the increase of fluorescent signal. The assay can be applied to real-time detection of disintegration with advantages of simplicity, high sensitivity, and excellent specificity. Copyright © 2013 Elsevier Inc. All rights reserved.

Radio/FADS/IMU integrated navigation for Mars entry

NASA Astrophysics Data System (ADS)

Jiang, Xiuqiang; Li, Shuang; Huang, Xiangyu

2018-03-01

Supposing future orbiting and landing collaborative exploration mission as the potential project background, this paper addresses the issue of Mars entry integrated navigation using radio beacon, flush air data sensing system (FADS), and inertial measurement unit (IMU). The range and Doppler information sensed from an orbiting radio beacon, the dynamic pressure and heating data sensed from flush air data sensing system, and acceleration and attitude angular rate outputs from an inertial measurement unit are integrated in an unscented Kalman filter to perform state estimation and suppress the system and measurement noise. Computer simulations show that the proposed integrated navigation scheme can enhance the navigation accuracy, which enables precise entry guidance for the given Mars orbiting and landing collaborative exploration mission.

Miniaturization of the atmospheric laser communication APT system

NASA Astrophysics Data System (ADS)

Sun, Wei; Ai, Yong; Yang, Jinling; Huang, Haibo

2003-09-01

The paper presents a scheme of the miniaturization of APT system and the design of the system based on the investigation of status in quo. It deals with the infrared image of the other terminal's beacon from the Charge Coupled Device (CCD) by the Complex Programmable Logic Device (CPLD). The result of the transaction is delivered to Single Chip Microcomputer (SCM), which controls the micro-servomotor. Subsequently, the precision drive system drives the optical system that uses only one light axis for signal beam and beacon to finish the acquisition, pointing, and tracking of the communication terminals. The anlayses of the APT system's error indicate that the tracking error limits in 70uRad with the weight of the system lighter than 8-kilogram.

Emergency Lightning System

NASA Astrophysics Data System (ADS)

1980-01-01

Super Vacuum Manufacturing Company's Stem-Lite Emergency Lighting System is widely used by fire, police, ambulance and other emergency service departments. The lights -- four floodlights which provide 2,000 watts of daytime equivalent visibility and a high-intensity flashing beacon can be elevated 10 feet above the roof of an emergency vehicle by means of an extendible mast. The higher elevation expands the effective radius of the floodlights and increases the beacon's visibility to several miles affording extra warning time to approaching traffic. When not in use, the light can be retracted into the compact rooftop housing. Stem-Lite also includes a generator which can serve to power such emergency equipment as pumps and drills, and a dashboard-mounted control panel for switching the lights and extending or retracting the mast.

Potential effects of the introduction of the discrete address beacon system data link on air/ground information transfer problems

NASA Technical Reports Server (NTRS)

Grayson, R. L.

1981-01-01

This study of Aviation Safety Reporting System reports suggests that benefits should accure from implementation of discrete address beacon system data link. The phase enhanced terminal information system service is expected to provide better terminal information than present systems by improving currency and accuracy. In the exchange of air traffic control messages, discrete address insures that only the intended recipient receives and acts on a specific message. Visual displays and printer copy of messages should mitigate many of the reported problems associated with voice communications. The problems that remain unaffected include error in addressing the intended recipient and messages whose content is wrong but are otherwise correct as to format and reasonableness.

CENet: A Cabinet Environmental Sensing Network

PubMed Central

Zhang, Zusheng; Yu, Fengqi; Chen, Liang; Cao, Guangmin

2010-01-01

For data center cooling and intelligent substation systems, real time cabinet environmental monitoring is a strong requirement. Monitoring data, such as temperature, humidity, and noise, is important for operators to manage the facilities in cabinets. We here propose a sensing network, called CENet, which is energy efficient and reliable for cabinet environmental monitoring. CENet achieves above 93% reliable data yield and sends fewer beacons compared to periodic beaconing. It does so through a data-aided routing protocol. In addition, based on B-MAC, we propose a scheduling scheme to increase the lifetime of the network by reducing unnecessary message snooping and channel listening, thus it is more energy efficient than B-MAC. The performance of CENet is evaluated by simulations and experiments. PMID:22205856

Rationale and trial design of Bardoxolone Methyl Evaluation in Patients with Chronic Kidney Disease and Type 2 Diabetes: the Occurrence of Renal Events (BEACON).

PubMed

de Zeeuw, Dick; Akizawa, Tadao; Agarwal, Rajiv; Audhya, Paul; Bakris, George L; Chin, Melanie; Krauth, Melissa; Lambers Heerspink, Hiddo J; Meyer, Colin J; McMurray, John J; Parving, Hans-Henrik; Pergola, Pablo E; Remuzzi, Giuseppe; Toto, Robert D; Vaziri, Nosratola D; Wanner, Christoph; Warnock, David G; Wittes, Janet; Chertow, Glenn M

2013-01-01

Chronic kidney disease (CKD) associated with type 2 diabetes mellitus constitutes a global epidemic complicated by considerable renal and cardiovascular morbidity and mortality, despite the provision of inhibitors of the renin-angiotensin-aldosterone system (RAAS). Bardoxolone methyl, a synthetic triterpenoid that reduces oxidative stress and inflammation through Nrf2 activation and inhibition of NF-κB was previously shown to increase estimated glomerular filtration rate (eGFR) in patients with CKD associated with type 2 diabetes mellitus. To date, no antioxidant or anti-inflammatory therapy has proved successful at slowing the progression of CKD. Herein, we describe the design of Bardoxolone Methyl Evaluation in Patients with Chronic Kidney Disease and Type 2 Diabetes: the Occurrence of Renal Events (BEACON) trial, a multinational, multicenter, double-blind, randomized, placebo-controlled Phase 3 trial designed to determine whether long-term administration of bardoxolone methyl (on a background of standard therapy, including RAAS inhibitors) safely reduces renal and cardiac morbidity and mortality. The primary composite endpoint is time-to-first occurrence of either end-stage renal disease or cardiovascular death. Secondary endpoints include the change in eGFR and time to occurrence of cardiovascular events. BEACON will be the first event-driven trial to evaluate the effect of an oral antioxidant and anti-inflammatory drug in advanced CKD. Copyright © 2013 S. Karger AG, Basel.

Rapid and amplification-free detection of fish pathogens by utilizing a molecular beacon-based microfluidic system.

PubMed

Su, Yi-Chih; Wang, Chih-Hung; Chang, Wen-Hsin; Chen, Tzong-Yueh; Lee, Gwo-Bin

2015-01-15

Nervous necrosis virus (NNV) and iridovirus are highly infectious pathogens that can cause lethal diseases in various species of fish. These infectious diseases have no effective treatments and the mortality rate is over 80%, which could cause dramatic economic losses in the aquaculture industry. Conventional diagnostic methods of NNV or iridovirus infected fishes, such as virus culture, enzyme-linked immunosorbent assays and nucleic acid assays usually require time-consuming and complex procedures performed by specialized technicians with delicate laboratory facilities. Rapid, simple, accurate and on-site detection of NNV and iridovirus infections would enable timely preventive measures such as immediate sacrifice of infected fishes, and is therefore critically needed for the aquaculture industry. In this study, a microfluidic-based assay that employ magnetic beads conjugated with viral deoxyribonucleic acid (DNA) capturing probes and fluorescent DNA molecular beacons were developed to rapidly detect NNV and iridovirus. Importantly, this new assay was realized in an integrated microfluidic system with a custom-made control system. With this approach, direct and automated NNV and iridovirus detection from infected fishes can be achieved in less than 30 min. Therefore, this molecular-beacon based microfluidic system presents a potentially promising tool for rapid diagnosis of fish pathogens in the field in the future. Copyright © 2014 Elsevier B.V. All rights reserved.

Long-stem shaped multifunctional molecular beacon for highly sensitive nucleic acids determination via intramolecular and intermolecular interactions based strand displacement amplification.

PubMed

Xu, Jianguo; Zheng, Tingting; Le, Jingqing; Jia, Lee

2017-11-20

Occurrence and application of oligonucleotide probes have promoted great progress in the biochemical analysis field due to their unique biological and chemical properties. In this work, a long-stem shaped multifunctional molecular beacon (LS-MMB) that is responsive to a cancer-related gene, p53, is well-prepared. By designing the probe with long-paired bases at its two ends and short-paired bases between the middle region and the 3' end, the LS-MMB is intelligently endowed with the ability to recognize the target analyte, serve as the polymerization primer/template, and signal the hybridization event synchronously, which is distinctly advantageous over the traditional molecular beacons (MBs). Moreover, it is excitingly found that the LS-MMB can be employed to exert intramolecular and intermolecular interactions for strand displacement amplification (SDA) without the involvement of any assistant probes; this therapy results in a really easy and rapid sensing system that provides an extremely low background noise and high target output signal. In this case, an excellent sensitivity and specificity to detect target gene down to picomolar level and resolution to even one nucleotide variation are achieved, respectively. In addition, the application potential for real genomic DNA analysis is realized. We envision that the probe of LS-MMB can act as a universal platform for biosensing and biomedical research.

A Localization Method for Underwater Wireless Sensor Networks Based on Mobility Prediction and Particle Swarm Optimization Algorithms

PubMed Central

Zhang, Ying; Liang, Jixing; Jiang, Shengming; Chen, Wei

2016-01-01

Due to their special environment, Underwater Wireless Sensor Networks (UWSNs) are usually deployed over a large sea area and the nodes are usually floating. This results in a lower beacon node distribution density, a longer time for localization, and more energy consumption. Currently most of the localization algorithms in this field do not pay enough consideration on the mobility of the nodes. In this paper, by analyzing the mobility patterns of water near the seashore, a localization method for UWSNs based on a Mobility Prediction and a Particle Swarm Optimization algorithm (MP-PSO) is proposed. In this method, the range-based PSO algorithm is used to locate the beacon nodes, and their velocities can be calculated. The velocity of an unknown node is calculated by using the spatial correlation of underwater object’s mobility, and then their locations can be predicted. The range-based PSO algorithm may cause considerable energy consumption and its computation complexity is a little bit high, nevertheless the number of beacon nodes is relatively smaller, so the calculation for the large number of unknown nodes is succinct, and this method can obviously decrease the energy consumption and time cost of localizing these mobile nodes. The simulation results indicate that this method has higher localization accuracy and better localization coverage rate compared with some other widely used localization methods in this field. PMID:26861348

Palindromic Molecule Beacon-Based Cascade Amplification for Colorimetric Detection of Cancer Genes.

PubMed

Shen, Zhi-Fa; Li, Feng; Jiang, Yi-Fan; Chen, Chang; Xu, Huo; Li, Cong-Cong; Yang, Zhe; Wu, Zai-Sheng

2018-03-06

A highly sensitive and selective colorimetric assay based on a multifunctional molecular beacon with palindromic tail (PMB) was proposed for the detection of target p53 gene. The PMB probe can serve as recognition element, primer, and polymerization template and contains a nicking site and a C-rich region complementary to a DNAzyme. In the presence of target DNA, the hairpin of PMB is opened, and the released palindromic tails intermolecularly hybridize with each other, triggering the autonomous polymerization/nicking/displacement cycles. Although only one type of probe is involved, the system can execute triple and continuous polymerization strand displacement amplifications, generating large amounts of G-quadruplex fragments. These G-rich fragments can bind to hemin and form the DNAzymes that possess the catalytic activity similar to horseradish peroxidase, catalyzing the oxidation of ABTS by H 2 O 2 and producing the colorimetric signal. Utilizing the newly proposed sensing system, target DNA can be detected down to 10 pM with a linear response range from 10 pM to 200 nM, and mutant target DNAs are able to be distinguished even by the naked eye. The desirable detection sensitivity, high specificity, and operation convenience without any separation step and chemical modification demonstrate that the palindromic molecular beacon holds the potential for detecting and monitoring a variety of nucleic acid-related biomarkers.

Status of ARGOS - The Laser Guide Star System for the LBT

NASA Astrophysics Data System (ADS)

Raab, Walfried; Rabien, Sebastian; Gaessler, Wolfgang; Esposito, Simone; Antichi, Jacopo; Lloyd-Hart, Michael; Barl, Lothar; Beckmann, Udo; Bonaglia, Marco; Borelli, Jose; Brynnel, Joar; Buschkamp, Peter; Busoni, Lorenzo; Carbonaro, Luca; Christou, Julian; Connot, Claus; Davies, Richard; Deysenroth, Matthias; Durney, Olivier; Green, Richard; Gemperlein, Hans; Gasho, Victor; Haug, Marcus; Hubbard, Pete; Ihle, Sebastian; Kulas, Martin; Loose, Christina; Lehmitz, Michael; Noenickx, Jamison; Nussbaum, Edmund; Orban De Xivry, Gilles; Quirrenbach, Andreas; Peter, Diethard; Rahmer, Gustavo; Rademacher, Matt; Storm, Jesper; Schwab, Christian; Vaitheeswaran, Vidhya; Ziegleder, Julian

2013-12-01

ARGOS is an innovative multiple laser guide star adaptive optics system for the Large Binocular Telescope (LBT), designed to perform effective GLAO correction over a very wide field of view. The system is using high powered pulsed green (532 nm) lasers to generate a set of three guide stars above each of the LBT mirrors. The laser beams are launched through a 40 cm telescope and focused at an altitude of 12 km, creating laser beacons by means of Rayleigh scattering. The returning scattered light, primarily sensitive to the turbulences close to the ground, is detected by a gated wavefront sensor system. The derived ground layer correction signals are directly driving the adaptive secondary mirror of the LBT. ARGOS is especially designed for operation with the multiple object spectrograph Luci, which will benefit from both, the improved spatial resolution, as well as the strongly enhanced flux. In addition to the GLAO Rayleigh beacon system, ARGOS was also designed for a possible future upgrade with a hybrid sodium laser - Rayleigh beacon combination, enabling diffraction limited operation. The ARGOS laser system has undergone extensive tests during Summer 2012 and is scheduled for installation at the LBT in Spring 2013. The remaining sub-systems will be installed during the course of 2013. We report on the overall status of the ARGOS system and the results of the sub-system characterizations carried out so far.

Twelve Foot Subsatellite

NASA Image and Video Library

1958-04-25

Engineer and 12 foot Beacon showing NACA emblem on inflated satelloon . For related information see, Spaceflight Revolution, NASA from Sputnik to Apollo, by James R. Hansen. NASA SP-4308, 1995. p. 173.

Creating accessible science museums with user-activated environmental audio beacons (ping!).

PubMed

Landau, Steven; Wiener, William; Naghshineh, Koorosh; Giusti, Ellen

2005-01-01

In 2003, Touch Graphics Company carried out research on a new invention that promises to improve accessibility to science museums for visitors who are visually impaired. The system, nicknamed Ping!, allows users to navigate an exhibit area, listen to audio descriptions, and interact with exhibits using a cell phone-based interface. The system relies on computer telephony, and it incorporates a network of wireless environmental audio beacons that can be triggered by users wishing to travel to destinations they choose. User testing indicates that the system is effective, both as a way-finding tool and as a means of providing accessible information on museum content. Follow-up development projects will determine if this approach can be successfully implemented in other settings and for other user populations.

Molecular beacon-based photodynamic therapy

NASA Astrophysics Data System (ADS)

Chen, Juan; Stefflova, Klara; Kim, Soungkyoo; Li, Hui; Marotta, Diane; Chance, Britton; Glickson, Jerry D.; Zheng, Gang

2005-01-01

A new concept for photodynamic therapy (PDT) has been developed based on incorporating a photosensitizer (PS) and a singlet oxygen (1O2) quenching/scavenging molecule (Q) onto a disease-targeting carrier, such that the PS becomes activatable by light only when targeting has occurred. This has the potential to give very high disease specificity in PDT treatment. The first model compound designed using this concept was synthesized containing a pyropheophorbide as the PS and a carotenoid as the 1O2 quencher. These were kept in close proximity by the self-folding of a caspase-3 specific peptide sequence. Upon caspase-3-induced cleavage, the 1O2 production increase has been validated by direct 1O2 luminescence and lifetime measurements, providing proof-of-concept of this 'PDT beacon.'

Model-Based Localization and Tracking Using Bluetooth Low-Energy Beacons

PubMed Central

Cemgil, Ali Taylan

2017-01-01

We introduce a high precision localization and tracking method that makes use of cheap Bluetooth low-energy (BLE) beacons only. We track the position of a moving sensor by integrating highly unreliable and noisy BLE observations streaming from multiple locations. A novel aspect of our approach is the development of an observation model, specifically tailored for received signal strength indicator (RSSI) fingerprints: a combination based on the optimal transport model of Wasserstein distance. The tracking results of the entire system are compared with alternative baseline estimation methods, such as nearest neighboring fingerprints and an artificial neural network. Our results show that highly accurate estimation from noisy Bluetooth data is practically feasible with an observation model based on Wasserstein distance interpolation combined with the sequential Monte Carlo (SMC) method for tracking. PMID:29109375

Model-Based Localization and Tracking Using Bluetooth Low-Energy Beacons.

PubMed

Daniş, F Serhan; Cemgil, Ali Taylan

2017-10-29

We introduce a high precision localization and tracking method that makes use of cheap Bluetooth low-energy (BLE) beacons only. We track the position of a moving sensor by integrating highly unreliable and noisy BLE observations streaming from multiple locations. A novel aspect of our approach is the development of an observation model, specifically tailored for received signal strength indicator (RSSI) fingerprints: a combination based on the optimal transport model of Wasserstein distance. The tracking results of the entire system are compared with alternative baseline estimation methods, such as nearest neighboring fingerprints and an artificial neural network. Our results show that highly accurate estimation from noisy Bluetooth data is practically feasible with an observation model based on Wasserstein distance interpolation combined with the sequential Monte Carlo (SMC) method for tracking.

Advanced TIL system for laser beam focusing in a turbulent regime

NASA Astrophysics Data System (ADS)

Sprangle, Phillip A.; Ting, Antonio C.; Kaganovich, Dmitry; Khizhnyak, Anatoliy I.; Tomov, Ivan V.; Markov, Vladimir B.; Korobkin, Dmitriy V.

2014-10-01

This paper discusses an advanced target in the loop (ATIL) system with its performance based on a nonlinear phase conjugation scheme that performs rapid adjustment of the laser beam wavefront to mitigate effects associated with atmospheric turbulence along the propagation path. The ATIL method allows positional control of the laser spot (the beacon) on a remote imaged-resolved target. The size of this beacon is governed by the reciprocity of two counterpropagating beams (one towards the target and another scattered by the target) and the fidelity of the phase conjugation scheme. In this presentation we will present the results of the thorough analysis of ATIL operation, factors that affect its performance, its focusing efficiency and the comparison of laboratory experimental validation and computer simulation results.

Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe.

PubMed

Ma, Changbei; Lv, Xiaoyuan; Wang, Kemin; Jin, Shunxin; Liu, Haisheng; Wu, Kefeng; Zeng, Weimin

2017-11-02

Protein kinase A was detected by quantifying the amount of ATP used after a protein kinase reaction. The ATP assay was performed using the T4 DNA ligase and a molecular beacon (MB). In the presence of ATP, DNA ligase catalyzed the ligation of short DNA. The ligation product then hybridized to MB, resulting in a fluorescence enhancement of the MB. This assay was capable of determining protein kinase A in the range of 12.5∼150 nM, with a detection limit of 1.25 nM. Furthermore, this assay could also be used to investigate the effect of genistein on protein kinase A. It was a universal, non-radioisotopic, and homogeneous method for assaying protein kinase A.

Sensitive detection of narrowband pulses.

PubMed

Cullers, D K

1986-01-01

Highly monochromatic signals, such as TV carriers, can be detected sensitively by using a narrow filter (b < or = 1 Hz) and performing power accumulation on its output. If instead a low-duty-cycle pulsed signal of the same total energy is present, the sensitivity of a square law device, followed by a thresholding operation (to eliminate most samples containing only noise), followed by the algorithm to be described, is greater by about 7 dB in typical cases. This is particularly interesting to SETI because such a pulsed signal is exactly what is sent by a rotating beacon with a directional antenna. Such a pulsed signal is, therefore, a good candidate for an extraterrestrial beacon. Software for detecting this signal type is now ready for field testing with the NASA Multichannel Spectrum Analyzer (MCSA).

A label-free colorimetric isothermal cascade amplification for the detection of disease-related nucleic acids based on double-hairpin molecular beacon.

PubMed

Wu, Dong; Xu, Huo; Shi, Haimei; Li, Weihong; Sun, Mengze; Wu, Zai-Sheng

2017-03-08

K-Ras mutations at codon 12 play an important role in an early step of carcinogenesis. Here, a label-free colorimetric isothermal cascade amplification for ultrasensitive and specific detection of K-Ras point mutation is developed based on a double-hairpin molecular beacon (DHMB). The biosensor consists of DHMB probe and a primer-incorporated polymerization template (PPT) designed partly complementary to DHMB. In the presence of polymerase, target DNA is designed to trigger strand displacement amplification (SDA) via promote the hybridization of PPT with DHMB and subsequently initiates cascade amplification process with the help of the nicking endonuclease. During the hybridization and enzymatic reaction, G-quadruplex/hemin DNAzymes are generated, catalyzing the oxidation of ABTS 2- by H 2 O 2 in the presence of hemin. Utilizing the proposed facile colorimetric scheme, the target DNA can be quantified down to 4 pM with the dynamic response range of 5 orders of magnitude, indicating the substantially improved detection capability. Even more strikingly, point mutation in K-ras gene can be readily observed by the naked eye without the need for the labeling or expensive equipment. Given the high-performance for K-Ras analysis, the enhanced signal transduction capability associated with double-hairpin structure of DHMB provides a novel rout to screen biomarkers, and the descripted colorimetric biosensor seems to hold great promise for diagnostic applications of genetic diseases. Copyright © 2016 Elsevier B.V. All rights reserved.

High resolution SETI: Experiences and prospects

NASA Astrophysics Data System (ADS)

Horowitz, Paul; Clubok, Ken

Megachannel spectroscopy with sub-Hertz resolution constitutes an attractive strategy for a microwave search for extraterrestrial intelligence (SETI), assuming the transmission of a narrowband radiofrequency beacon. Such resolution matches the properties of the interstellar medium, and the necessary Doppler corrections provide a high degree of interference rejection. We have constructed a frequency-agile receiver with an FFT-based 8 megachannel digital spectrum analyzer, on-line signal recognition, and multithreshold archiving. We are using it to conduct a meridian transit search of the northern sky at the Harvard-Smithsonian 26-m antenna, with a second identical system scheduled to begin observations in Argentina this month. Successive 400 kHz spectra, at 0.05 Hz resolution, are searched for features characteristic of an intentional narrowband beacon transmission. These spectra are centered on guessable frequencies (such as λ21 cm), referenced successively to the local standard of rest, the galactic barycenter, and the cosmic blackbody rest frame. This search has rejected interference admirably, but is greatly limited both in total frequency coverage and sensitivity to signals other than carriers. We summarize five years of high resolution SETI at Harvard, in the context of answering the questions "How useful is narrowband SETI, how serious are its limitations, what can be done to circumvent them, and in what direction should SETI evolve?" Increasingly powerful signal processing hardware, combined with ever-higher memory densities, are particularly relevant, permitting the construction of compact and affordable gigachannel spectrum analyzers covering hundreds of megahertz of instantaneous bandwidth.

Human place and response learning: navigation strategy selection, pupil size and gaze behavior.

PubMed

de Condappa, Olivier; Wiener, Jan M

2016-01-01

In this study, we examined the cognitive processes and ocular behavior associated with on-going navigation strategy choice using a route learning paradigm that distinguishes between three different wayfinding strategies: an allocentric place strategy, and the egocentric associative cue and beacon response strategies. Participants approached intersections of a known route from a variety of directions, and were asked to indicate the direction in which the original route continued. Their responses in a subset of these test trials allowed the assessment of strategy choice over the course of six experimental blocks. The behavioral data revealed an initial maladaptive bias for a beacon response strategy, with shifts in favor of the optimal configuration place strategy occurring over the course of the experiment. Response time analysis suggests that the configuration strategy relied on spatial transformations applied to a viewpoint-dependent spatial representation, rather than direct access to an allocentric representation. Furthermore, pupillary measures reflected the employment of place and response strategies throughout the experiment, with increasing use of the more cognitively demanding configuration strategy associated with increases in pupil dilation. During test trials in which known intersections were approached from different directions, visual attention was directed to the landmark encoded during learning as well as the intended movement direction. Interestingly, the encoded landmark did not differ between the three navigation strategies, which is discussed in the context of initial strategy choice and the parallel acquisition of place and response knowledge.

Use of a text message program to raise type 2 diabetes risk awareness and promote health behavior change (part II): assessment of participants' perceptions on efficacy.

PubMed

Buis, Lorraine R; Hirzel, Lindsey; Turske, Scott A; Des Jardins, Terrisca R; Yarandi, Hossein; Bondurant, Patricia

2013-12-19

Although there is great enthusiasm in both the public and private sector for the further development and use of large-scale consumer-facing public health applications for mobile platforms, little is known about user experience and satisfaction with this type of approach. As a part of the Beacon Community Cooperative Agreement Program, txt4health, a public-facing, mobile phone-based health information service targeting type 2 diabetes, was launched in 3 Beacon Communities: the Southeast Michigan Beacon Community in Detroit, MI, the Greater Cincinnati Beacon Community in Cincinnati, OH, and the Crescent City Beacon Community in New Orleans, LA. This program was marketed via large public health campaigns and drew many users within the respective communities. The purpose of this investigation was to use the RE-AIM framework to document txt4health efficacy by focusing on perceptions of satisfaction, usage, and behavior change among individuals who used txt4health in pilot studies in Southeast Michigan and Greater Cincinnati. We conducted a multimodal user survey with txt4health users recruited via text message through the program to understand participant perceptions of program use and satisfaction, as well as self-reported perceptions of behavior change as a result of using txt4health. Txt4health users reported very high levels of program satisfaction, with 67.1% (108/161) reporting satisfaction scores of ≥8 on a 10-point scale, with 10 equivalent to most satisfied (mean 8.2, SD 1.6). All survey participants agreed/strongly agreed that the messages included in txt4health were clear and easy to understand (100.0%, 160/160), and most found txt4health made them knowledgeable about their risk for type 2 diabetes (88.1%, 140/159) and made them conscious of their diet and physical activity (88.8%, 142/160). Most participants reported that txt4health helped them to make behavior changes related to diet; after having completed txt4health, most agreed/strongly agreed that they are more likely to replace sugary drinks, such as juice or soda, with water (78.0%, 124/159), have a piece of fresh fruit instead of dessert (74.2%, 118/159), substitute a small salad for chips or fries when dining out (76.1%, 121/159), buy healthier foods when grocery shopping (79.7%, 126/158), and eat more grilled, baked, or broiled foods instead of fried (75.5%, 120/159). Results from this study suggest that participants in txt4health, a large-scale, public health-focused text message program targeting type 2 diabetes, have positive perceptions of the program and that participation has led to positive behavior change.

47 CFR 97.203 - Beacon station.

Code of Federal Regulations, 2013 CFR

2013-10-01

... written notification thereof to the Interference Office, National Radio Astronomy Observatory, P.O. Box 2... the proposed operation is received by the FCC from the National Radio Astronomy Observatory at Green...

47 CFR 97.203 - Beacon station.

Code of Federal Regulations, 2011 CFR

2011-10-01

... written notification thereof to the Interference Office, National Radio Astronomy Observatory, P.O. Box 2... the proposed operation is received by the FCC from the National Radio Astronomy Observatory at Green...