Performance analysis of bi-directional broadband passive optical network using erbium-doped fiber amplifier

NASA Astrophysics Data System (ADS)

Almalaq, Yasser; Matin, Mohammad A.

2014-09-01

The broadband passive optical network (BPON) has the ability to support high-speed data, voice, and video services to home and small businesses customers. In this work, the performance of bi-directional BPON is analyzed for both down and up streams traffic cases by the help of erbium doped fiber amplifier (EDFA). The importance of BPON is reduced cost. Because PBON uses a splitter the cost of the maintenance between the providers and the customers side is suitable. In the proposed research, BPON has been tested by the use of bit error rate (BER) analyzer. BER analyzer realizes maximum Q factor, minimum bit error rate, and eye height.

Cardinality enhancement utilizing Sequential Algorithm (SeQ) code in OCDMA system

NASA Astrophysics Data System (ADS)

Fazlina, C. A. S.; Rashidi, C. B. M.; Rahman, A. K.; Aljunid, S. A.

2017-11-01

Optical Code Division Multiple Access (OCDMA) has been important with increasing demand for high capacity and speed for communication in optical networks because of OCDMA technique high efficiency that can be achieved, hence fibre bandwidth is fully used. In this paper we will focus on Sequential Algorithm (SeQ) code with AND detection technique using Optisystem design tool. The result revealed SeQ code capable to eliminate Multiple Access Interference (MAI) and improve Bit Error Rate (BER), Phase Induced Intensity Noise (PIIN) and orthogonally between users in the system. From the results, SeQ shows good performance of BER and capable to accommodate 190 numbers of simultaneous users contrast with existing code. Thus, SeQ code have enhanced the system about 36% and 111% of FCC and DCS code. In addition, SeQ have good BER performance 10-25 at 155 Mbps in comparison with 622 Mbps, 1 Gbps and 2 Gbps bit rate. From the plot graph, 155 Mbps bit rate is suitable enough speed for FTTH and LAN networks. Resolution can be made based on the superior performance of SeQ code. Thus, these codes will give an opportunity in OCDMA system for better quality of service in an optical access network for future generation's usage

Finite-SNR analysis for partial relaying cooperation with channel coding and opportunistic relay selection

NASA Astrophysics Data System (ADS)

Vu, Thang X.; Duhamel, Pierre; Chatzinotas, Symeon; Ottersten, Bjorn

2017-12-01

This work studies the performance of a cooperative network which consists of two channel-coded sources, multiple relays, and one destination. To achieve high spectral efficiency, we assume that a single time slot is dedicated to relaying. Conventional network-coded-based cooperation (NCC) selects the best relay which uses network coding to serve the two sources simultaneously. The bit error rate (BER) performance of NCC with channel coding, however, is still unknown. In this paper, we firstly study the BER of NCC via a closed-form expression and analytically show that NCC only achieves diversity of order two regardless of the number of available relays and the channel code. Secondly, we propose a novel partial relaying-based cooperation (PARC) scheme to improve the system diversity in the finite signal-to-noise ratio (SNR) regime. In particular, closed-form expressions for the system BER and diversity order of PARC are derived as a function of the operating SNR value and the minimum distance of the channel code. We analytically show that the proposed PARC achieves full (instantaneous) diversity order in the finite SNR regime, given that an appropriate channel code is used. Finally, numerical results verify our analysis and demonstrate a large SNR gain of PARC over NCC in the SNR region of interest.

Broadening Participation in Biology Education Research: Engaging Community College Students and Faculty.

PubMed

Schinske, Jeffrey N; Balke, Virginia L; Bangera, M Gita; Bonney, Kevin M; Brownell, Sara E; Carter, Robert S; Curran-Everett, Douglas; Dolan, Erin L; Elliott, Samantha L; Fletcher, Linnea; Gonzalez, Beatriz; Gorga, Joseph J; Hewlett, James A; Kiser, Stacey L; McFarland, Jenny L; Misra, Anjali; Nenortas, Apryl; Ngeve, Smith M; Pape-Lindstrom, Pamela A; Seidel, Shannon B; Tuthill, Matthew C; Yin, Yue; Corwin, Lisa A

2017-01-01

Nearly half of all undergraduates are enrolled at community colleges (CCs), including the majority of U.S. students who represent groups underserved in the sciences. Yet only a small minority of studies published in discipline-based education research journals address CC biology students, faculty, courses, or authors. This marked underrepresentation of CC biology education research (BER) limits the availability of evidence that could be used to increase CC student success in biology programs. To address this issue, a diverse group of stakeholders convened at the Building Capacity for Biology Education Research at Community Colleges meeting to discuss how to increase the prevalence of CC BER and foster participation of CC faculty as BER collaborators and authors. The group identified characteristics of CCs that make them excellent environments for studying biology teaching and learning, including student diversity and institutional cultures that prioritize teaching, learning, and assessment. The group also identified constraints likely to impede BER at CCs: limited time, resources, support, and incentives, as well as misalignment between doing research and CC faculty identities as teachers. The meeting culminated with proposing strategies for faculty, administrators, journal editors, scientific societies, and funding agencies to better support CC BER. © 2017 J. N. Schinske et al. CBE—Life Sciences Education © 2017 The American Society for Cell Biology. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

OF MICROBES AND MEN: A SPECIAL REPORT IN THE JOURNAL FOR MINORITY MEDICAL STUDENTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

BILL BOWERS

2008-11-12

In support of the mission for the Office of Science and the Office of Biological and Environmental Research (BER), Spectrum Publishers proposes an editorial project to inform and educate minority undergraduate students in the sciences, minority medical students and minority medical residents of the opportunities and challenges available to them as they complete their training. This editorial project will take the form of a 32-page insert in the Journal for Minority Medical Students. The subject matter will be determined by BER based on mission requirements. The material will be compiled, assembled, edited, revised, designed, printed and distributed as a totalmore » package with a vast majority of the work performed by our staff. Our objective is to provide the special report without added (and burdensome) work to the BER staff. The 32-page report will be distributed to our readership of 10,000 future scientists and physicians. In addition, we will prepare the insert so that it can also be used by BER as a stand-alone piece and outreach tool. After publication, we will solicit feedback from our readers through our unique Campus Rep Program of students strategically located on campuses across the nation who will provide valuable editorial feedback. This innovative program will give BER a quick read on the effectiveness of its message. The total cost for this mission-related project is only $30,000.00. Based on our earlier experience with DOE, we are confident that this level of funding will be sufficient to develop an effective educational campaign.« less

Differential Characteristics Based Iterative Multiuser Detection for Wireless Sensor Networks

PubMed Central

Chen, Xiaoguang; Jiang, Xu; Wu, Zhilu; Zhuang, Shufeng

2017-01-01

High throughput, low latency and reliable communication has always been a hot topic for wireless sensor networks (WSNs) in various applications. Multiuser detection is widely used to suppress the bad effect of multiple access interference in WSNs. In this paper, a novel multiuser detection method based on differential characteristics is proposed to suppress multiple access interference. The proposed iterative receive method consists of three stages. Firstly, a differential characteristics function is presented based on the optimal multiuser detection decision function; then on the basis of differential characteristics, a preliminary threshold detection is utilized to find the potential wrongly received bits; after that an error bit corrector is employed to correct the wrong bits. In order to further lower the bit error ratio (BER), the differential characteristics calculation, threshold detection and error bit correction process described above are iteratively executed. Simulation results show that after only a few iterations the proposed multiuser detection method can achieve satisfactory BER performance. Besides, BER and near far resistance performance are much better than traditional suboptimal multiuser detection methods. Furthermore, the proposed iterative multiuser detection method also has a large system capacity. PMID:28212328

Optical multiple access techniques for on-board routing

NASA Technical Reports Server (NTRS)

Mendez, Antonio J.; Park, Eugene; Gagliardi, Robert M.

1992-01-01

The purpose of this research contract was to design and analyze an optical multiple access system, based on Code Division Multiple Access (CDMA) techniques, for on board routing applications on a future communication satellite. The optical multiple access system was to effect the functions of a circuit switch under the control of an autonomous network controller and to serve eight (8) concurrent users at a point to point (port to port) data rate of 180 Mb/s. (At the start of this program, the bit error rate requirement (BER) was undefined, so it was treated as a design variable during the contract effort.) CDMA was selected over other multiple access techniques because it lends itself to bursty, asynchronous, concurrent communication and potentially can be implemented with off the shelf, reliable optical transceivers compatible with long term unattended operations. Temporal, temporal/spatial hybrids and single pulse per row (SPR, sometimes termed 'sonar matrices') matrix types of CDMA designs were considered. The design, analysis, and trade offs required by the statement of work selected a temporal/spatial CDMA scheme which has SPR properties as the preferred solution. This selected design can be implemented for feasibility demonstration with off the shelf components (which are identified in the bill of materials of the contract Final Report). The photonic network architecture of the selected design is based on M(8,4,4) matrix codes. The network requires eight multimode laser transmitters with laser pulses of 0.93 ns operating at 180 Mb/s and 9-13 dBm peak power, and 8 PIN diode receivers with sensitivity of -27 dBm for the 0.93 ns pulses. The wavelength is not critical, but 830 nm technology readily meets the requirements. The passive optical components of the photonic network are all multimode and off the shelf. Bit error rate (BER) computations, based on both electronic noise and intercode crosstalk, predict a raw BER of (10 exp -3) when all eight users are communicating concurrently. If better BER performance is required, then error correction codes (ECC) using near term electronic technology can be used. For example, the M(8,4,4) optical code together with Reed-Solomon (54,38,8) encoding provides a BER of better than (10 exp -11). The optical transceiver must then operate at 256 Mb/s with pulses of 0.65 ns because the 'bits' are now channel symbols.

Creep-induced anisotropy in covalent adaptable network polymers.

PubMed

Hanzon, Drew W; He, Xu; Yang, Hua; Shi, Qian; Yu, Kai

2017-10-11

Anisotropic polymers with aligned macromolecule chains exhibit directional strengthening of mechanical and physical properties. However, manipulating the orientation of polymer chains in a fully cured thermoset is almost impossible due to its permanently crosslinked nature. In this paper, we demonstrate that rearrangeable networks with bond exchange reactions (BERs) can be utilized to tailor the anisotropic mechanical properties of thermosetting polymers. When a constant force is maintained at BER activated temperatures, the malleable thermoset creeps in the direction of stress, and macromolecule chains align themselves in the same direction. The aligned polymer chains result in an anisotropic network with a stiffer mechanical behavior in the direction of creep, while with a more compliant behavior in the transverse direction. The degree of network anisotropy is proportional to the amount of creep strain. A multi-length scale constitutive model is developed to study the creep-induced anisotropy of thermosetting polymers. The model connects the micro-scale BER kinetics, orientation of polymer chains, and directional mechanical properties of network polymers. Without any fitting parameters, it is able to predict the evolution of creep strain at different temperatures and anisotropic stress-strain behaviors of CANs after creep. Predictions on the chain orientation are verified by molecular dynamics (MD) simulation. Based on parametric studies, it is shown that the influences of creep time and temperature on the network anisotropy can be generalized into a single parameter, and the evolution of directional modulus follows an Arrhenius type time-temperature superposition principle (TTSP). The presented work provides a facile approach to transform isotropic thermosets into anisotropic ones using simple heating, and their directional properties can be readily tailored by the processing conditions.

Broadening Participation in Biology Education Research: Engaging Community College Students and Faculty

PubMed Central

Schinske, Jeffrey N.; Balke, Virginia L.; Bangera, M. Gita; Bonney, Kevin M.; Brownell, Sara E.; Carter, Robert S.; Curran-Everett, Douglas; Dolan, Erin L.; Elliott, Samantha L.; Fletcher, Linnea; Gonzalez, Beatriz; Gorga, Joseph J.; Hewlett, James A.; Kiser, Stacey L.; McFarland, Jenny L.; Misra, Anjali; Nenortas, Apryl; Ngeve, Smith M.; Pape-Lindstrom, Pamela A.; Seidel, Shannon B.; Tuthill, Matthew C.; Yin, Yue; Corwin, Lisa A.

2017-01-01

Nearly half of all undergraduates are enrolled at community colleges (CCs), including the majority of U.S. students who represent groups underserved in the sciences. Yet only a small minority of studies published in discipline-based education research journals address CC biology students, faculty, courses, or authors. This marked underrepresentation of CC biology education research (BER) limits the availability of evidence that could be used to increase CC student success in biology programs. To address this issue, a diverse group of stakeholders convened at the Building Capacity for Biology Education Research at Community Colleges meeting to discuss how to increase the prevalence of CC BER and foster participation of CC faculty as BER collaborators and authors. The group identified characteristics of CCs that make them excellent environments for studying biology teaching and learning, including student diversity and institutional cultures that prioritize teaching, learning, and assessment. The group also identified constraints likely to impede BER at CCs: limited time, resources, support, and incentives, as well as misalignment between doing research and CC faculty identities as teachers. The meeting culminated with proposing strategies for faculty, administrators, journal editors, scientific societies, and funding agencies to better support CC BER. PMID:28450448

Medical reliable network using concatenated channel codes through GSM network.

PubMed

Ahmed, Emtithal; Kohno, Ryuji

2013-01-01

Although the 4(th) generation (4G) of global mobile communication network, i.e. Long Term Evolution (LTE) coexisting with the 3(rd) generation (3G) has successfully started; the 2(nd) generation (2G), i.e. Global System for Mobile communication (GSM) still playing an important role in many developing countries. Without any other reliable network infrastructure, GSM can be applied for tele-monitoring applications, where high mobility and low cost are necessary. A core objective of this paper is to introduce the design of a more reliable and dependable Medical Network Channel Code system (MNCC) through GSM Network. MNCC design based on simple concatenated channel code, which is cascade of an inner code (GSM) and an extra outer code (Convolution Code) in order to protect medical data more robust against channel errors than other data using the existing GSM network. In this paper, the MNCC system will provide Bit Error Rate (BER) equivalent to the BER for medical tele monitoring of physiological signals, which is 10(-5) or less. The performance of the MNCC has been proven and investigated using computer simulations under different channels condition such as, Additive White Gaussian Noise (AWGN), Rayleigh noise and burst noise. Generally the MNCC system has been providing better performance as compared to GSM.

10-Gbps optical duobinary signal generated by bandwidth-limited reflective semiconductor optical amplifier in colorless optical network units and compensated by fiber Bragg grating-based equalizer in optical line terminal

NASA Astrophysics Data System (ADS)

Fu, Meixia; Zhang, Min; Wang, Danshi; Cui, Yue; Han, Huanhuan

2016-10-01

We propose a scheme of optical duobinary-modulated upstream transmission system for reflective semiconductor optical amplifier-based colorless optical network units in 10-Gbps wavelength-division multiplexed passive optical network (WDM-PON), where a fiber Bragg grating (FBG) is adopted as an optical equalizer for better performance. The demodulation module is extremely simple, only needing a binary intensity modulation direct detection receiver. A better received sensitivity of -16.98 dBm at bit rate error (BER)=1.0×10-4 can be achieved at 120 km without FBG, and the BER at the sensitivity of -18.49 dBm can be up to 2.1×10-5 at the transmission distance of 160 km with FBG, which demonstrates the feasibility of our proposed scheme. Moreover, it could be a high cost-effectiveness scheme for WDM-PON in the future.

Spread Spectrum Based Energy Efficient Collaborative Communication in Wireless Sensor Networks.

PubMed

Ghani, Anwar; Naqvi, Husnain; Sher, Muhammad; Khan, Muazzam Ali; Khan, Imran; Irshad, Azeem

2016-01-01

Wireless sensor networks consist of resource limited devices. Most crucial of these resources is battery life, as in most applications like battle field or volcanic area monitoring, it is often impossible to replace or recharge the power source. This article presents an energy efficient collaborative communication system based on spread spectrum to achieve energy efficiency as well as immunity against jamming, natural interference, noise suppression and universal frequency reuse. Performance of the proposed system is evaluated using the received signal power, bit error rate (BER) and energy consumption. The results show a direct proportionality between the power gain and the number of collaborative nodes as well as BER and signal-to-noise ratio (Eb/N0). The analytical and simulation results of the proposed system are compared with SISO system. The comparison reveals that SISO perform better than collaborative communication in case of small distances whereas collaborative communication performs better than SISO in case of long distances. On the basis of these results it is safe to conclude that collaborative communication in wireless sensor networks using wideband systems improves the life time of nodes in the networks thereby prolonging the network's life time.

Dynamic quality of service differentiation using fixed code weight in optical CDMA networks

NASA Astrophysics Data System (ADS)

Kakaee, Majid H.; Essa, Shawnim I.; Abd, Thanaa H.; Seyedzadeh, Saleh

2015-11-01

The emergence of network-driven applications, such as internet, video conferencing, and online gaming, brings in the need for a network the environments with capability of providing diverse Quality of Services (QoS). In this paper, a new code family of novel spreading sequences, called a Multi-Service (MS) code, has been constructed to support multiple services in Optical- Code Division Multiple Access (CDMA) system. The proposed method uses fixed weight for all services, however reducing the interfering codewords for the users requiring higher QoS. The performance of the proposed code is demonstrated using mathematical analysis. It shown that the total number of served users with satisfactory BER of 10-9 using NB=2 is 82, while they are only 36 and 10 when NB=3 and 4 respectively. The developed MS code is compared with variable-weight codes such as Variable Weight-Khazani Syed (VW-KS) and Multi-Weight-Random Diagonal (MW-RD). Different numbers of basic users (NB) are used to support triple-play services (audio, data and video) with different QoS requirements. Furthermore, reference to the BER of 10-12, 10-9, and 10-3 for video, data and audio, respectively, the system can support up to 45 total users. Hence, results show that the technique can clearly provide a relative QoS differentiation with lower value of basic users can support larger number of subscribers as well as better performance in terms of acceptable BER of 10-9 at fixed code weight.

VCSEL-based fiber optic link for avionics: implementation and performance analyses

NASA Astrophysics Data System (ADS)

Shi, Jieqin; Zhang, Chunxi; Duan, Jingyuan; Wen, Huaitao

2006-11-01

A Gb/s fiber optic link with built-in test capability (BIT) basing on vertical-cavity surface-emitting laser (VCSEL) sources for military avionics bus for next generation has been presented in this paper. To accurately predict link performance, statistical methods and Bit Error Rate (BER) measurements have been examined. The results show that the 1Gb/s fiber optic link meets the BER requirement and values for link margin can reach up to 13dB. Analysis shows that the suggested photonic network may provide high performance and low cost interconnections alternative for future military avionics.

Performance Analysis of Relay Subset Selection for Amplify-and-Forward Cognitive Relay Networks

PubMed Central

Qureshi, Ijaz Mansoor; Malik, Aqdas Naveed; Zubair, Muhammad

2014-01-01

Cooperative communication is regarded as a key technology in wireless networks, including cognitive radio networks (CRNs), which increases the diversity order of the signal to combat the unfavorable effects of the fading channels, by allowing distributed terminals to collaborate through sophisticated signal processing. Underlay CRNs have strict interference constraints towards the secondary users (SUs) active in the frequency band of the primary users (PUs), which limits their transmit power and their coverage area. Relay selection offers a potential solution to the challenges faced by underlay networks, by selecting either single best relay or a subset of potential relay set under different design requirements and assumptions. The best relay selection schemes proposed in the literature for amplify-and-forward (AF) based underlay cognitive relay networks have been very well studied in terms of outage probability (OP) and bit error rate (BER), which is deficient in multiple relay selection schemes. The novelty of this work is to study the outage behavior of multiple relay selection in the underlay CRN and derive the closed-form expressions for the OP and BER through cumulative distribution function (CDF) of the SNR received at the destination. The effectiveness of relay subset selection is shown through simulation results. PMID:24737980

Study of bidirectional broadband passive optical network (BPON) using EDFA

NASA Astrophysics Data System (ADS)

Almalaq, Yasser

Optical line terminals (OLTs) and number of optical network units (ONUs) are two main parts of passive optical network (PON). OLT is placed at the central office of the service providers, the ONUs are located near to the end subscribers. When compared with point-to-point design, a PON decreases the number of fiber used and central office components required. Broadband PON (BPON), which is one type of PON, can support high-speed voice, data and video services to subscribers' residential homes and small businesses. In this research, by using erbium doped fiber amplifier (EDFA), the performance of bi-directional BPON is experimented and tested for both downstream and upstream traffic directions. Ethernet PON (E-PON) and gigabit PON (G-PON) are the two other kinds of passive optical network besides BPON. The most beneficial factor of using BPON is it's reduced cost. The cost of the maintenance between the central office and the users' side is suitable because of the use of passive components, such as a splitter in the BPON architecture. In this work, a bidirectional BPON has been analyzed for both downstream and upstream cases by using bit error rate analyzer (BER). BER analyzers test three factors that are the maximum Q factor, minimum bit error rate, and eye height. In other words, parameters such as maximum Q factor, minimum bit error rate, and eye height can be analyzed utilized a BER tester. Passive optical components such as a splitter, optical circulator, and filters have been used in modeling and simulations. A 12th edition Optiwave simulator has been used in order to analyze the bidirectional BPON system. The system has been tested under several conditions such as changing the fiber length, extinction ratio, dispersion, and coding technique. When a long optical fiber above 40km was used, an EDFA was used in order to improve the quality of the signal.

Dissolution of covalent adaptable network polymers in organic solvent

NASA Astrophysics Data System (ADS)

Yu, Kai; Yang, Hua; Dao, Binh H.; Shi, Qian; Yakacki, Christopher M.

2017-12-01

It was recently reported that thermosetting polymers can be fully dissolved in a proper organic solvent utilizing a bond-exchange reaction (BER), where small molecules diffuse into the polymer, break the long polymer chains into short segments, and eventually dissolve the network when sufficient solvent is provided. The solvent-assisted dissolution approach was applied to fully recycle thermosets and their fiber composites. This paper presents the first multi-scale modeling framework to predict the dissolution kinetics and mechanics of thermosets in organic solvent. The model connects the micro-scale network dynamics with macro-scale material properties: in the micro-scale, a model is developed based on the kinetics of BERs to describe the cleavage rate of polymer chains and evolution of chain segment length during the dissolution. The micro-scale model is then fed into a continuum-level model with considerations of the transportation of solvent molecules and chain segments in the system. The model shows good prediction on conversion rate of functional groups, degradation of network mechanical properties, and dissolution rate of thermosets during the dissolution. It identifies the underlying kinetic factors governing the dissolution process, and reveals the influence of different material and processing variables on the dissolution process, such as time, temperature, catalyst concentration, and chain length between cross-links.

Performance Analysis of Hybrid PON (WDM-TDM) with Equal and Unequal Channel Spacing

NASA Astrophysics Data System (ADS)

Sharma, Ramandeep; Dewra, Sanjeev; Rani, Aruna

2016-06-01

In this hybrid WDM-TDM PON has been evaluated and compared the downstream wavelengths with equal and unequal channel spacing at 5 Gbit/s per wavelength in the scenario of triple play services with 128 optical network units (ONUs). The triple play services: data, voice and video signals are transmitted up to 50 km distance having Q factor of 6.68 and BER of 3.64e-012 with unequal channel spacing and 45 km distance having Q factor of 6.33 and BER of 2.40e-011 with equal channel spacing in downstream direction. It has been observed that downstream wavelengths with unequal channel spacing provide better results than equal channel spacing.

Using Fabry-Perot laser diode and reflective semiconductor optical amplifier for long reach WDM-PON system

NASA Astrophysics Data System (ADS)

Yeh, C. H.; Chow, C. W.; Wu, Y. F.; Shih, F. Y.; Chi, S.

2011-10-01

In this investigation, we propose and investigate the simple self-injection locked Fabry-Perot laser diodes (FP-LDs) in optical line terminal (OLT); and wavelength-tunable optical network unit (ONU) using reflective optical semiconductor amplifier (RSOA) and FP-LD laser for downstream and upstream traffic in long reach (LR) wavelength division multiplexed-passive optical network (WDM-PON) respectively. The output performance of the proposed two laser sources in terms of power and side-mode suppression ratio (SMSR) has been discussed. Here, for the downstream traffic, the proposed optical transmitter can be directly modulated at 2.5 Gb/s on-off keying (OOK) format with nearly 0.4 dB power penalty at bit error rate (BER) of 10 -9 through 75 km single-mode fiber (SMF) transmission. Moreover, the proposed upstream transmitter can be directly modulated at 1.25 and 2.5 Gb/s with nearly 0.5 and 1.1 dB power penalty, respectively, at the BER of 10 -9.

Cooperative MIMO communication at wireless sensor network: an error correcting code approach.

PubMed

Islam, Mohammad Rakibul; Han, Young Shin

2011-01-01

Cooperative communication in wireless sensor network (WSN) explores the energy efficient wireless communication schemes between multiple sensors and data gathering node (DGN) by exploiting multiple input multiple output (MIMO) and multiple input single output (MISO) configurations. In this paper, an energy efficient cooperative MIMO (C-MIMO) technique is proposed where low density parity check (LDPC) code is used as an error correcting code. The rate of LDPC code is varied by varying the length of message and parity bits. Simulation results show that the cooperative communication scheme outperforms SISO scheme in the presence of LDPC code. LDPC codes with different code rates are compared using bit error rate (BER) analysis. BER is also analyzed under different Nakagami fading scenario. Energy efficiencies are compared for different targeted probability of bit error p(b). It is observed that C-MIMO performs more efficiently when the targeted p(b) is smaller. Also the lower encoding rate for LDPC code offers better error characteristics.

Cooperative MIMO Communication at Wireless Sensor Network: An Error Correcting Code Approach

PubMed Central

Islam, Mohammad Rakibul; Han, Young Shin

2011-01-01

Cooperative communication in wireless sensor network (WSN) explores the energy efficient wireless communication schemes between multiple sensors and data gathering node (DGN) by exploiting multiple input multiple output (MIMO) and multiple input single output (MISO) configurations. In this paper, an energy efficient cooperative MIMO (C-MIMO) technique is proposed where low density parity check (LDPC) code is used as an error correcting code. The rate of LDPC code is varied by varying the length of message and parity bits. Simulation results show that the cooperative communication scheme outperforms SISO scheme in the presence of LDPC code. LDPC codes with different code rates are compared using bit error rate (BER) analysis. BER is also analyzed under different Nakagami fading scenario. Energy efficiencies are compared for different targeted probability of bit error pb. It is observed that C-MIMO performs more efficiently when the targeted pb is smaller. Also the lower encoding rate for LDPC code offers better error characteristics. PMID:22163732

A network of enzymes involved in repair of oxidative DNA damage in Neisseria meningitidis

PubMed Central

Li, Yanwen; Pelicic, Vladimir; Freemont, Paul S.; Baldwin, Geoff S.; Tang, Christoph M.

2013-01-01

Although oxidative stress is a key aspect of innate immunity, little is known about how host-restricted pathogens successfully repair DNA damage. Base excision repair (BER) is responsible for correcting nucleobases damaged by oxidative stress, and is essential for bloodstream infection caused by the human pathogen, Neisseria meningitidis. We have characterised meningococcal BER enzymes involved in the recognition and removal of damaged nucleobases, and incision of the DNA backbone. We demonstrate that the bi-functional glycosylase/lyases Nth and MutM share several overlapping activities and functional redundancy. However MutM and other members of the GO system, which deal with 8-oxoG, a common lesion of oxidative damage, are not required for survival of N. meningitidis under oxidative stress. Instead, the mismatch repair pathway provides back-up for the GO system, while the lyase activity of Nth can substitute for the meningococcal AP endonuclease, NApe. Our genetic and biochemical evidence show that DNA repair is achieved through a robust network of enzymes that provides a flexible system of DNA repair. This network is likely to reflect successful adaptation to the human nasopharynx, and might provide a paradigm for DNA repair in other prokaryotes. PMID:22296581

Performance enhancement of optical code-division multiple-access systems using transposed modified Walsh code

NASA Astrophysics Data System (ADS)

Sikder, Somali; Ghosh, Shila

2018-02-01

This paper presents the construction of unipolar transposed modified Walsh code (TMWC) and analysis of its performance in optical code-division multiple-access (OCDMA) systems. Specifically, the signal-to-noise ratio, bit error rate (BER), cardinality, and spectral efficiency were investigated. The theoretical analysis demonstrated that the wavelength-hopping time-spreading system using TMWC was robust against multiple-access interference and more spectrally efficient than systems using other existing OCDMA codes. In particular, the spectral efficiency was calculated to be 1.0370 when TMWC of weight 3 was employed. The BER and eye pattern for the designed TMWC were also successfully obtained using OptiSystem simulation software. The results indicate that the proposed code design is promising for enhancing network capacity.

Contributions of the Atmospheric Radiation Measurement (ARM) Program and the ARM Climate Research Facility to the U.S. Climate Change Science Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

SA Edgerton; LR Roeder

The Earth’s surface temperature is determined by the balance between incoming solar radiation and thermal (or infrared) radiation emitted by the Earth back to space. Changes in atmospheric composition, including greenhouse gases, clouds, and aerosols can alter this balance and produce significant climate change. Global climate models (GCMs) are the primary tool for quantifying future climate change; however, there remain significant uncertainties in the GCM treatment of clouds, aerosol, and their effects on the Earth’s energy balance. The 2007 assessment (AR4) by the Intergovernmental Panel on Climate Change (IPCC) reports a substantial range among GCMs in climate sensitivity to greenhousemore » gas emissions. The largest contributor to this range lies in how different models handle changes in the way clouds absorb or reflect radiative energy in a changing climate (Solomon et al. 2007). In 1989, the U.S. Department of Energy (DOE) Office of Science created the Atmospheric Radiation Measurement (ARM) Program within the Office of Biological and Environmental Research (BER) to address scientific uncertainties related to global climate change, with a specific focus on the crucial role of clouds and their influence on the transfer of radiation in the atmosphere. To address this problem, BER has adopted a unique two-pronged approach: * The ARM Climate Research Facility (ACRF), a scientific user facility for obtaining long-term measurements of radiative fluxes, cloud and aerosol properties, and related atmospheric characteristics in diverse climate regimes. * The ARM Science Program, focused on the analysis of ACRF data to address climate science issues associated with clouds, aerosols, and radiation, and to improve GCMs. This report describes accomplishments of the BER ARM Program toward addressing the primary uncertainties related to climate change prediction as identified by the IPCC.« less

Investigation on iterative multiuser detection physical layer network coding in two-way relay free-space optical links with turbulences and pointing errors.

PubMed

Abu-Almaalie, Zina; Ghassemlooy, Zabih; Bhatnagar, Manav R; Le-Minh, Hoa; Aslam, Nauman; Liaw, Shien-Kuei; Lee, It Ee

2016-11-20

Physical layer network coding (PNC) improves the throughput in wireless networks by enabling two nodes to exchange information using a minimum number of time slots. The PNC technique is proposed for two-way relay channel free space optical (TWR-FSO) communications with the aim of maximizing the utilization of network resources. The multipair TWR-FSO is considered in this paper, where a single antenna on each pair seeks to communicate via a common receiver aperture at the relay. Therefore, chip interleaving is adopted as a technique to separate the different transmitted signals at the relay node to perform PNC mapping. Accordingly, this scheme relies on the iterative multiuser technique for detection of users at the receiver. The bit error rate (BER) performance of the proposed system is examined under the combined influences of atmospheric loss, turbulence-induced channel fading, and pointing errors (PEs). By adopting the joint PNC mapping with interleaving and multiuser detection techniques, the BER results show that the proposed scheme can achieve a significant performance improvement against the degrading effects of turbulences and PEs. It is also demonstrated that a larger number of simultaneous users can be supported with this new scheme in establishing a communication link between multiple pairs of nodes in two time slots, thereby improving the channel capacity.

Reed-Muller Codes in Error Correction in Wireless Adhoc Networks

DTIC Science & Technology

2004-03-01

resulting spectrum is the spectrum of the windowed signal. Therefore, the window width is an important pa- rameter that affects the BER performanceof ... compare the results, the same random message was used. The seed value in msg.m was changed only for comparing the PAPR values of the system with

Performance analysis of cooperative virtual MIMO systems for wireless sensor networks.

PubMed

Rafique, Zimran; Seet, Boon-Chong; Al-Anbuky, Adnan

2013-05-28

Multi-Input Multi-Output (MIMO) techniques can be used to increase the data rate for a given bit error rate (BER) and transmission power. Due to the small form factor, energy and processing constraints of wireless sensor nodes, a cooperative Virtual MIMO as opposed to True MIMO system architecture is considered more feasible for wireless sensor network (WSN) applications. Virtual MIMO with Vertical-Bell Labs Layered Space-Time (V-BLAST) multiplexing architecture has been recently established to enhance WSN performance. In this paper, we further investigate the impact of different modulation techniques, and analyze for the first time, the performance of a cooperative Virtual MIMO system based on V-BLAST architecture with multi-carrier modulation techniques. Through analytical models and simulations using real hardware and environment settings, both communication and processing energy consumptions, BER, spectral efficiency, and total time delay of multiple cooperative nodes each with single antenna are evaluated. The results show that cooperative Virtual-MIMO with Binary Phase Shift Keying-Wavelet based Orthogonal Frequency Division Multiplexing (BPSK-WOFDM) modulation is a promising solution for future high data-rate and energy-efficient WSNs.

Performance Analysis of Cooperative Virtual MIMO Systems for Wireless Sensor Networks

PubMed Central

Rafique, Zimran; Seet, Boon-Chong; Al-Anbuky, Adnan

2013-01-01

Multi-Input Multi-Output (MIMO) techniques can be used to increase the data rate for a given bit error rate (BER) and transmission power. Due to the small form factor, energy and processing constraints of wireless sensor nodes, a cooperative Virtual MIMO as opposed to True MIMO system architecture is considered more feasible for wireless sensor network (WSN) applications. Virtual MIMO with Vertical-Bell Labs Layered Space-Time (V-BLAST) multiplexing architecture has been recently established to enhance WSN performance. In this paper, we further investigate the impact of different modulation techniques, and analyze for the first time, the performance of a cooperative Virtual MIMO system based on V-BLAST architecture with multi-carrier modulation techniques. Through analytical models and simulations using real hardware and environment settings, both communication and processing energy consumptions, BER, spectral efficiency, and total time delay of multiple cooperative nodes each with single antenna are evaluated. The results show that cooperative Virtual-MIMO with Binary Phase Shift Keying-Wavelet based Orthogonal Frequency Division Multiplexing (BPSK-WOFDM) modulation is a promising solution for future high data-rate and energy-efficient WSNs. PMID:23760087

Universal method for crosstalk noise and transmission loss analysis for N-port nonblocking optical router for photonic networks-on-chip

NASA Astrophysics Data System (ADS)

Xie, Yiyuan; Zhang, Zhendong; Song, Tingting; He, Chao; Li, Jiachao; Wang, Guijin

2016-05-01

Crosstalk noise and transmission loss are two key elements in determining the performance of optical routers. We propose a universal method for crosstalk noise and transmission loss analysis for the N-port nonblocking optical router used in photonic networks-on-chip. Utilizing this method, we study the crosstalk noise and transmission loss for the five-, six-, seven-, and eight-port optical routers. We ascertain that the crosstalk noise and transmission loss are different for different input-output pairs. For the five-port optical router, the maximum crosstalk noise ranges from 0 to -7.07 dBm, and the transmission loss ranges from -9.05 to -0.51 dB. Furthermore, based on the crosstalk noise and transmission loss, we analyze optical signal-to-noise ratio (OSNR) and bit error ratio (BER) for the five-, six-, seven-, and eight-port nonblocking optical routers. As the number of ports increases, the minimum average OSNR decreases and the average BER increases. In addition, in order to present the performance of the routers more visually, a fiber-optic communications system is designed to simulate the transmission processes of the signals of the different paths of the routers in Optisystem. The results show that the power amplitude of the input signal is obviously higher than the corresponding output signal. With this method, we can easily evaluate the transmission loss, crosstalk noise, OSNR, and BER of high-radix nonblocking optical routers and conveniently study the performance of the N-port optical router.

Big Biology: Supersizing Science During the Emergence of the 21st Century

PubMed Central

Vermeulen, Niki

2017-01-01

Ist Biologie das jüngste Mitglied in der Familie von Big Science? Die vermehrte Zusammenarbeit in der biologischen Forschung wurde in der Folge des Human Genome Project zwar zum Gegenstand hitziger Diskussionen, aber Debatten und Reflexionen blieben meist im Polemischen verhaftet und zeigten eine begrenzte Wertschätzung für die Vielfalt und Erklärungskraft des Konzepts von Big Science. Zur gleichen Zeit haben Wissenschafts- und Technikforscher/innen in ihren Beschreibungen des Wandels der Forschungslandschaft die Verwendung des Begriffs Big Science gemieden. Dieser interdisziplinäre Artikel kombiniert eine begriffliche Analyse des Konzepts von Big Science mit unterschiedlichen Daten und Ideen aus einer Multimethodenuntersuchung mehrerer großer Forschungsprojekte in der Biologie. Ziel ist es, ein empirisch fundiertes, nuanciertes und analytisch nützliches Verständnis von Big Biology zu entwickeln und die normativen Debatten mit ihren einfachen Dichotomien und rhetorischen Positionen hinter sich zu lassen. Zwar kann das Konzept von Big Science als eine Mode in der Wissenschaftspolitik gesehen werden – inzwischen vielleicht sogar als ein altmodisches Konzept –, doch lautet meine innovative Argumentation, dass dessen analytische Verwendung unsere Aufmerksamkeit auf die Ausweitung der Zusammenarbeit in den Biowissenschaften lenkt. Die Analyse von Big Biology zeigt Unterschiede zu Big Physics und anderen Formen von Big Science, namentlich in den Mustern der Forschungsorganisation, der verwendeten Technologien und der gesellschaftlichen Zusammenhänge, in denen sie tätig ist. So können Reflexionen über Big Science, Big Biology und ihre Beziehungen zur Wissensproduktion die jüngsten Behauptungen über grundlegende Veränderungen in der Life Science-Forschung in einen historischen Kontext stellen. PMID:27215209

Peaceful Uses of the Atom and Atoms for Peace

Science.gov Websites

power plants. Converting Energy to Medical Progress [Nuclear Medicine] The DOE Office of Biological and Environmental Research (BER) Medical Sciences program fosters research that develops beneficial applications of nuclear technologies for medical diagnosis and treatment of many diseases. The First Weighing of Plutonium

Biology Education Research Trends in Turkey

ERIC Educational Resources Information Center

Gul, Seyda; Sozbilir, Mustafa

2015-01-01

This paper reports on a content analysis of 633 biology education research [BER] papers published by Turkish science educators in national and international journals. The findings indicate that more research has been undertaken in environment and ecology, the cell and animal form and functions. In addition learning, teaching and attitudes were in…

Two-dimensional optoelectronic interconnect-processor and its operational bit error rate

NASA Astrophysics Data System (ADS)

Liu, J. Jiang; Gollsneider, Brian; Chang, Wayne H.; Carhart, Gary W.; Vorontsov, Mikhail A.; Simonis, George J.; Shoop, Barry L.

2004-10-01

Two-dimensional (2-D) multi-channel 8x8 optical interconnect and processor system were designed and developed using complementary metal-oxide-semiconductor (CMOS) driven 850-nm vertical-cavity surface-emitting laser (VCSEL) arrays and the photodetector (PD) arrays with corresponding wavelengths. We performed operation and bit-error-rate (BER) analysis on this free-space integrated 8x8 VCSEL optical interconnects driven by silicon-on-sapphire (SOS) circuits. Pseudo-random bit stream (PRBS) data sequence was used in operation of the interconnects. Eye diagrams were measured from individual channels and analyzed using a digital oscilloscope at data rates from 155 Mb/s to 1.5 Gb/s. Using a statistical model of Gaussian distribution for the random noise in the transmission, we developed a method to compute the BER instantaneously with the digital eye-diagrams. Direct measurements on this interconnects were also taken on a standard BER tester for verification. We found that the results of two methods were in the same order and within 50% accuracy. The integrated interconnects were investigated in an optoelectronic processing architecture of digital halftoning image processor. Error diffusion networks implemented by the inherently parallel nature of photonics promise to provide high quality digital halftoned images.

The Role of Margin in Link Design and Optimization

NASA Technical Reports Server (NTRS)

Cheung, K.

2015-01-01

Link analysis is a system engineering process in the design, development, and operation of communication systems and networks. Link models that are mathematical abstractions representing the useful signal power and the undesirable noise and attenuation effects (including weather effects if the signal path transverses through the atmosphere) that are integrated into the link budget calculation that provides the estimates of signal power and noise power at the receiver. Then the link margin is applied which attempts to counteract the fluctuations of the signal and noise power to ensure reliable data delivery from transmitter to receiver. (Link margin is dictated by the link margin policy or requirements.) A simple link budgeting approach assumes link parameters to be deterministic values typically adopted a rule-of-thumb policy of 3 dB link margin. This policy works for most S- and X-band links due to their insensitivity to weather effects. But for higher frequency links like Ka-band, Ku-band, and optical communication links, it is unclear if a 3 dB link margin would guarantee link closure. Statistical link analysis that adopted the 2-sigma or 3-sigma link margin incorporates link uncertainties in the sigma calculation. (The Deep Space Network (DSN) link margin policies are 2-sigma for downlink and 3-sigma for uplink.) The link reliability can therefore be quantified statistically even for higher frequency links. However in the current statistical link analysis approach, link reliability is only expressed as the likelihood of exceeding the signal-to-noise ratio (SNR) threshold that corresponds to a given bit-error-rate (BER) or frame-error-rate (FER) requirement. The method does not provide the true BER or FER estimate of the link with margin, or the required signalto-noise ratio (SNR) that would meet the BER or FER requirement in the statistical sense. In this paper, we perform in-depth analysis on the relationship between BER/FER requirement, operating SNR, and coding performance curve, in the case when the channel coherence time of link fluctuation is comparable or larger than the time duration of a codeword. We compute the "true" SNR design point that would meet the BER/FER requirement by taking into account the fluctuation of signal power and noise power at the receiver, and the shape of the coding performance curve. This analysis yields a number of valuable insights on the design choices of coding scheme and link margin for the reliable data delivery of a communication system - space and ground. We illustrate the aforementioned analysis using a number of standard NASA error-correcting codes.

Revision of the stiletto fly genera Acupalpa Kröber and Pipinnipons Winterton (Diptera, Therevidae, Agapophytinae) using cybertaxonomic methods, with a key to Australasian genera

PubMed Central

Winterton, Shaun L.

2011-01-01

Abstract Australian stiletto flies of the sister-genera Acupalpa Kröber, 1912 and Pipinnipons Winterton, 2001 (Diptera: Therevidae: Agapophytinae) are revised. Twelve new species of Acupalpa are described, while Acupalpa imitans (White, 1915), comb. n. is transferred from Pipinnipons and Acupalpa albimanis (Kröber, 1914), comb. n. is transferred from Ectinorhynchus Macquart as a senior synonym of Acupalpa pollinosa Mann. The total number of species of Acupalpa is therefore increased to 19: Acupalpa albimanis (Kröber), comb. n., Acupalpa albitarsa Mann, Acupalpa boharti sp. n., Acupalpa divisa (Walker), Acupalpa dolichorhyncha sp. n., Acupalpa glossa sp. n., Acupalpa imitans (White), comb. n., Acupalpa irwini Winterton, Acupalpa melanophaeos sp. n., Acupalpa miaboolya sp. n., Acupalpa minuta sp. n., Acupalpa minutoides sp. n., Acupalpa notomelas sp. n., Acupalpa novayamarna sp. n., Acupalpa rostrata Kröber, Acupalpa semirufa Mann, Acupalpa westralica sp. n., Acupalpa yalgoo sp. n. and Acupalpa yanchep sp. n. Three new species of Pipinnipons are described, increasing the total number of species to five: Pipinnipons chauncyvallis sp. n., Pipinnipons fascipennis (Kröber), Pipinnipons kampmeierae sp. n., Pipinnipons kroeberi Winterton, and P. sphecoda sp. n. Pipinnipons and Acupalpa are rediagnosed in light of the new species presented herein and revised keys to species are included. A dichotomous key to genera of Australasian Therevidae is included. As an empirical example of cybertaxonomy, taxonomic descriptions were composed using a character matrix developed in Lucid Builder (in Structured Descriptive Data (SDD) format) to generate natural language descriptions supplemented by online specimen and image databases. Web resources are provided throughout the document including: a) links to high resolution colour images of all species on Morphbank, b) registration of authors, publications, taxon names and other nomenclatural acts in Zoobank, with assignment of Life Science Identifiers (LSIDs) for each, c) links to Genbank accession records for DNA sequences, and d) assignment of LSIDs to specimen records with links to respective records in an online Therevidae specimen database. PMID:21594068

Antibacterial and synergy of berberines with antibacterial agents against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA).

PubMed

Zuo, Guo-Ying; Li, Yang; Han, Jun; Wang, Gen-Chun; Zhang, Yun-Ling; Bian, Zhong-Qi

2012-08-29

Antibacterial activity of berberine (Ber) and 8-acetonyl-dihydroberberine (A-Ber) alone and combined uses with antibacterial agents ampicillin (AMP), azithromycin (AZM), cefazolin (CFZ) and levofloxacin (LEV) was studied on 10 clinical isolates of SCCmec III type methicillin-resistant Staphylococcus aureus (MRSA). Susceptibility to each agent alone was tested using a broth microdilution method and the chequerboard and time-kill tests for the combined evaluations, respectively. The alone MICs/MBCs (μg/mL) ranges were 32-128/64-256 (Ber) and 32-128/128-512 (A-Ber). Significant synergies were observed for the Ber (A-Ber)/AZM and Ber (A-Ber)/LEV combinations against 90% of the tested MRSA strains, with fractional inhibitory concentration indices (FICIs) values ranged from 0.188 to 0.500. An additivity result was also observed for the Ber/AZM combination by time-kill curves. These results demonstrated for the first time that Ber and A-Ber enhanced the in vitro inhibitory efficacy of AZM and LEV to a same extent, which had potential for further investigation in combinatory therapeutic applications of patients infected with MRSA.

Telecommunications end-to-end systems monitoring on TOPEX/Poseidon: Tools and techniques

NASA Technical Reports Server (NTRS)

Calanche, Bruno J.

1994-01-01

The TOPEX/Poseidon Project Satellite Performance Analysis Team's (SPAT) roles and responsibilities have grown to include functions that are typically performed by other teams on JPL Flight Projects. In particular, SPAT Telecommunication's role has expanded beyond the nominal function of monitoring, assessing, characterizing, and trending the spacecraft (S/C) RF/Telecom subsystem to one of End-to-End Information Systems (EEIS) monitoring. This has been accomplished by taking advantage of the spacecraft and ground data system structures and protocols. By processing both the received spacecraft telemetry minor frame ground generated CRC flags and NASCOM block poly error flags, bit error rates (BER) for each link segment can be determined. This provides the capability to characterize the separate link segments, determine science data recovery, and perform fault/anomaly detection and isolation. By monitoring and managing the links, TOPEX has successfully recovered approximately 99.9 percent of the science data with an integrity (BER) of better than 1 x 10(exp 8). This paper presents the algorithms used to process the above flags and the techniques used for EEIS monitoring.

Performance study on the effect of filter curve in CWDM System for the access network

NASA Astrophysics Data System (ADS)

Ali, N.; Rahman, N. A.; Hambali, N. A. M. Ahmad; Rashidi, C. B. M.

2017-11-01

This paper presents the study on the effect of filter variation on the coarse wavelength division multiplexing (CWDM) system. The filter curve will affect the performance of the CWDM system due to changes of received power lever and isolation of the signal. The significant impact on the received power level and isolation can be found when the required signal is isolated from unwanted signal by the steep curve of filter. As a result, BER of 1.0x 10-12 was obtained corresponding to receive power level of -24.27 dBm with isolation of 23.22 dB. When the wavelength spacing is reduced to 1nm, the isolation is only 11.30 dB and BER increased to 5.49x10-7 with a received power of -15.39 dBm.

Berberine and zinc oxide-based nanoparticles for the chemo-photothermal therapy of lung adenocarcinoma.

PubMed

Kim, Sungyun; Lee, Song Yi; Cho, Hyun-Jong

2018-05-16

Organic/inorganic hydrid nanoparticles (NPs) composed of berberine (BER) and zinc oxide (ZnO) were developed for the therapy of lung cancers. Without the use of pharmaceutical excipients, NPs were fabricated with only dual anticancer agents (BER and ZnO) by facile blending method. The mean weight ratio between BER and ZnO in BER-ZnO NPs was 39:61 in this study. BER-ZnO NPs dispersed in water exhibited 200-300 nm hydrodynamic size under 5 mg/mL concentration. The exposure of both BER and ZnO in the outer layers of BER-ZnO NPs was identified by X-ray photoelectron spectroscopy analysis. The amorphization of BER and the maintenance of ZnO structure were observed in the results of X-ray powder diffractometer analysis. Improved antiproliferation efficacy, based on the chemo-photothermal therapeutic efficacy, of BER-ZnO NPs in A549 (human lung adenocarcinoma) cells was presented. According to the blood tests in rats after intravenous administration, BER-ZnO NPs did not induce severe hepatotoxicity, renal toxicity, and hemotoxicity. Developed BER-ZnO NPs can be used efficiently and safely for the chemo-photothermal therapy of lung cancers. Copyright © 2018 Elsevier Inc. All rights reserved.

Long-reach transmission experiment of a wavelength division multiplexed-passive optical networks transmitter based on reflective semiconductor optical amplifiers

NASA Astrophysics Data System (ADS)

Jeon, Sie-Wook; Kim, Youngbok; Park, Chang-Soo

2012-01-01

We propose and demonstrate a long-reach wavelength division multiplexed-passive optical networks (WDM-PON) based on reflective semiconductor optical amplifiers (RSOAs) with easy maintenance of the optical source. Unlike previous studies the proposed WDM-PON uses two RSOAs: one for wavelength-selected light generation to provide a constant seed light to the second RSOA, the other for active external modulation. This method is free from intensity-fluctuated power penalties inherent to directly modulated single-RSOA sources, making long-reach transmission possible. Also, the wavelength of the modulated signal can easily be changed for the same RSOA by replacing the external feedback reflector, such as a fiber Bragg grating, or via thermal tuning. The seed light has a high-side-mode suppression ratio (SMSR) of 45 dB, and the bit error rate (BER) curve reveals that the upstream 1.25-Gb/s nonreturn-to-zero (NRZ) signal with a pseudo-random binary sequence (PRBS) of length of 215-1 has power penalties of 0.22 and 0.69 dB at BERs of 10-9 after 55-km and 110-km transmission due to fiber dispersion, respectively.

Channel coding for underwater acoustic single-carrier CDMA communication system

NASA Astrophysics Data System (ADS)

Liu, Lanjun; Zhang, Yonglei; Zhang, Pengcheng; Zhou, Lin; Niu, Jiong

2017-01-01

CDMA is an effective multiple access protocol for underwater acoustic networks, and channel coding can effectively reduce the bit error rate (BER) of the underwater acoustic communication system. For the requirements of underwater acoustic mobile networks based on CDMA, an underwater acoustic single-carrier CDMA communication system (UWA/SCCDMA) based on the direct-sequence spread spectrum is proposed, and its channel coding scheme is studied based on convolution, RA, Turbo and LDPC coding respectively. The implementation steps of the Viterbi algorithm of convolutional coding, BP and minimum sum algorithms of RA coding, Log-MAP and SOVA algorithms of Turbo coding, and sum-product algorithm of LDPC coding are given. An UWA/SCCDMA simulation system based on Matlab is designed. Simulation results show that the UWA/SCCDMA based on RA, Turbo and LDPC coding have good performance such that the communication BER is all less than 10-6 in the underwater acoustic channel with low signal to noise ratio (SNR) from -12 dB to -10dB, which is about 2 orders of magnitude lower than that of the convolutional coding. The system based on Turbo coding with Log-MAP algorithm has the best performance.

Two-Level Scheduling for Video Transmission over Downlink OFDMA Networks

PubMed Central

Tham, Mau-Luen

2016-01-01

This paper presents a two-level scheduling scheme for video transmission over downlink orthogonal frequency-division multiple access (OFDMA) networks. It aims to maximize the aggregate quality of the video users subject to the playback delay and resource constraints, by exploiting the multiuser diversity and the video characteristics. The upper level schedules the transmission of video packets among multiple users based on an overall target bit-error-rate (BER), the importance level of packet and resource consumption efficiency factor. Instead, the lower level renders unequal error protection (UEP) in terms of target BER among the scheduled packets by solving a weighted sum distortion minimization problem, where each user weight reflects the total importance level of the packets that has been scheduled for that user. Frequency-selective power is then water-filled over all the assigned subcarriers in order to leverage the potential channel coding gain. Realistic simulation results demonstrate that the proposed scheme significantly outperforms the state-of-the-art scheduling scheme by up to 6.8 dB in terms of peak-signal-to-noise-ratio (PSNR). Further test evaluates the suitability of equal power allocation which is the common assumption in the literature. PMID:26906398

A Guide for Graduate Students Interested in Postdoctoral Positions in Biology Education Research

ERIC Educational Resources Information Center

Aikens, Melissa L.; Corwin, Lisa A.; Andrews, Tessa C.; Couch, Brian A.; Eddy, Sarah L.; McDonnell, Lisa; Trujillo, Gloriana

2016-01-01

Postdoctoral positions in biology education research (BER) are becoming increasingly common as the field grows. However, many life science graduate students are unaware of these positions or do not understand what these positions entail or the careers with which they align. In this essay, we use a backward-design approach to inform life science…

In vitro and in vivo antitumor efficacy of berberine-nanostructured lipid carriers against H22 tumor

NASA Astrophysics Data System (ADS)

Wang, Zhi-ping; Wu, Jun-biao; Chen, Tong-sheng; Zhou, Qun; Wang, Yi-fei

2015-03-01

Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Berberine (Ber), an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Ber loaded nanostructured lipid carriers (Ber-NLC) was prepared by hot melting and then high pressure homogenization technique. Both in vitro and in vivo anti-hepatocarcinoma effects of Ber-NLC relative to efficacy of bulk Ber were evaluated. The particle size and zeta potential of Ber-NLC were 189.3 nm and -19.3 mV, respectively. MTT assay showed that Ber-NLC effectively inhibited the proliferation of H22 cells, and the corresponding IC50 values were 6.3 μg/ml (22.1 μg/ml of bulk Ber). In vivo studies also showed higher antitumor efficacy, and inhibition rates was 68.3 % (41.4 % of bulk Ber) at 100 mg/kg intragastric administration in the H22 solid tumor bearing mice. These results suggest that the delivery of Ber-NLC is a promising approach for treating tumors.

Anti-hepatocarcinoma effects of berberine nanosuspension against human HepG2 and Huh7 cells as well as H22 tumor bearing mice

NASA Astrophysics Data System (ADS)

Wang, Zhi-ping; Wu, Jun-biao; Zhou, Qun; Wang, Yi-fei; Chen, Tongsheng

2014-09-01

Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Berberine (Ber), an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Ber nanosuspension (Ber-NS) composed of Ber and D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) was prepared by high pressure homogenization technique. Both in vitro and in vivo anti-hepatocarcinoma effects of Ber-NS relative to effcacy of bulk Ber were evaluated. The particle size and zeta potential of Ber-NS were 73.1 +/- 3.7 nm and 6.99 +/- 0.17 mV, respectively. Ber-NS exhibited significant inhibitory effects against human HepG2 and Huh7 cells, and the corresponding IC50 values were 8.1 and 4.7 μg/ml (18.3 and 6.5 μg/ml of Ber solution). In vivo studies also showed higher antitumor efficacy, and inhibition rates was 63.7% (41.4 % of Ber solution) at 100 mg/kg intragastric administration in the H22 solid tumor bearing mice. These results suggest that the delivery of Ber as a nanosuspension is a promising approach for treating hepatocarcinoma.

Field-programmable gate array implementation of an all-digital IEEE 802.15.4-compliant transceiver

NASA Astrophysics Data System (ADS)

Cornetta, Gianluca; Touhafi, Abdellah; Santos, David J.; Vázquez, José M.

2010-12-01

An architecture for a low-cost, low-complexity digital transceiver is presented in this article. The proposed architecture targets the IEEE 802.15.4 standard for short-range wireless personal area networks and has been implemented as a synthesisable VHDL register transfer level description. The system has been evaluated and tested using a Xilinx 90 nm Virtex-4 field-programmable gate array as the target technology. Bit error rate (BER) and error vector magnitude (EVM) have been used as the figures of merit for modem performance. Simulations show that the recommended minimum BER is achieved at E b/N 0 = 8.7 dB, whereas the EVM is 19.5%. The implemented device occupies 10% of the target FPGA and has a normalised maximum power consumption of 44 mW in transmit mode and 53 mW in receiver mode.

Ka-Band Phased Array System Characterization

NASA Technical Reports Server (NTRS)

Acosta, R.; Johnson, S.; Sands, O.; Lambert, K.

2001-01-01

Phased Array Antennas (PAAs) using patch-radiating elements are projected to transmit data at rates several orders of magnitude higher than currently offered with reflector-based systems. However, there are a number of potential sources of degradation in the Bit Error Rate (BER) performance of the communications link that are unique to PAA-based links. Short spacing of radiating elements can induce mutual coupling between radiating elements, long spacing can induce grating lobes, modulo 2 pi phase errors can add to Inter Symbol Interference (ISI), phase shifters and power divider network introduce losses into the system. This paper describes efforts underway to test and evaluate the effects of the performance degrading features of phased-array antennas when used in a high data rate modulation link. The tests and evaluations described here uncover the interaction between the electrical characteristics of a PAA and the BER performance of a communication link.

Bandwidth efficient bidirectional 5 Gb/s overlapped-SCM WDM PON with electronic equalization and forward-error correction.

PubMed

Buset, Jonathan M; El-Sahn, Ziad A; Plant, David V

2012-06-18

We demonstrate an improved overlapped-subcarrier multiplexed (O-SCM) WDM PON architecture transmitting over a single feeder using cost sensitive intensity modulation/direct detection transceivers, data re-modulation and simple electronics. Incorporating electronic equalization and Reed-Solomon forward-error correction codes helps to overcome the bandwidth limitation of a remotely seeded reflective semiconductor optical amplifier (RSOA)-based ONU transmitter. The O-SCM architecture yields greater spectral efficiency and higher bit rates than many other SCM techniques while maintaining resilience to upstream impairments. We demonstrate full-duplex 5 Gb/s transmission over 20 km and analyze BER performance as a function of transmitted and received power. The architecture provides flexibility to network operators by relaxing common design constraints and enabling full-duplex operation at BER ∼ 10(-10) over a wide range of OLT launch powers from 3.5 to 8 dBm.

Amplify-and-forward cooperative diversity for green UWB-based WBSNs.

PubMed

Shaban, Heba; Abou El-Nasr, Mohamad

2013-01-01

This paper proposes a novel green cooperative diversity technique based on suboptimal template-based ultra-wideband (UWB) wireless body sensor networks (WBSNs) using amplify-and-forward (AF) relays. In addition, it analyzes the bit-error-rate (BER) performance of the proposed nodes. The analysis is based on the moment-generating function (MGF) of the total signal-to-noise ratio (SNR) at the destination. It also provides an approximate value for the total SNR. The analysis studies the performance of equally correlated binary pulse position modulation (EC-BPPM) assuming the sinusoidal and square suboptimal template pulses. Numerical results are provided for the performance evaluation of optimal and suboptimal template-based nodes with and without relay cooperation. Results show that one relay node provides ~23 dB performance enhancement at 1e - 3 BER, which mitigates the effect of the nondesirable non-line-of-sight (NLOS) links in WBSNs.

Amplify-and-Forward Cooperative Diversity for Green UWB-Based WBSNs

PubMed Central

2013-01-01

This paper proposes a novel green cooperative diversity technique based on suboptimal template-based ultra-wideband (UWB) wireless body sensor networks (WBSNs) using amplify-and-forward (AF) relays. In addition, it analyzes the bit-error-rate (BER) performance of the proposed nodes. The analysis is based on the moment-generating function (MGF) of the total signal-to-noise ratio (SNR) at the destination. It also provides an approximate value for the total SNR. The analysis studies the performance of equally correlated binary pulse position modulation (EC-BPPM) assuming the sinusoidal and square suboptimal template pulses. Numerical results are provided for the performance evaluation of optimal and suboptimal template-based nodes with and without relay cooperation. Results show that one relay node provides ~23 dB performance enhancement at 1e − 3 BER, which mitigates the effect of the nondesirable non-line-of-sight (NLOS) links in WBSNs. PMID:24307880

Base Excision Repair Variants in Cancer

PubMed Central

Marsden, Carolyn G.; Dragon, Julie A.; Wallace, Susan S.; Sweasy, Joann B.

2018-01-01

Base excision repair (BER) is a key genome maintenance pathway that removes endogenously damaged DNA bases that arise in cells at very high levels on a daily basis. Failure to remove these damaged DNA bases leads to increased levels of mutagenesis and chromosomal instability, which have the potential to drive carcinogenesis. Next Generation sequencing efforts of the germline and tumors genomes of thousands of individuals has uncovered many rare mutations in BER genes. Given that BER is critical for genome maintenance, it is important to determine whether BER genomic variants have functional phenotypes. In this chapter we present our in silico methods for the identification and prioritization of BER variants for further study. We also provide detailed instructions and commentary on the initial cellular assays we employ to dissect potentially important phenotypes of human BER variants and highlight the strengths and weaknesses of our approaches. BER variants possessing interesting functional phenotypes can then be studied in more detail to provide important mechanistic insights regarding the role of aberrant BER in carcinogenesis. PMID:28645367

International Trends in Biology Education Research from 1997 to 2014: A Content Analysis of Papers in Selected Journals

ERIC Educational Resources Information Center

Gul, Seyda; Sozbilir, Mustafa

2016-01-01

This paper provides a descriptive content analysis of biology education research papers published in eight major academic journals indexed in Social Science Citation Index [SSCI] of Thomson Reuters® from 1997 to 2014. Total of 1376 biology education research [BER] papers were examined. The findings indicated that most of the papers were published…

Turbulence mitigation scheme based on multiple-user detection in an orbital-angular-momentum multiplexed system

NASA Astrophysics Data System (ADS)

Zou, Li; Wang, Le; Zhao, Sheng-Mei; Chen, Han-Wu

2016-11-01

Atmospheric turbulence (AT) induced crosstalk can significantly impair the performance of a free-space optical (FSO) communication link using orbital angular momentum (OAM) multiplexing. In this paper, we propose a multiple-user detection (MUD) turbulence mitigation scheme in an OAM-multiplexed FSO communication link. First, we present a MUD equivalent communication model for an OAM-multiplexed FSO communication link under AT. In the equivalent model, each input bit stream represents one user’s information. The deformed OAM spatial modes caused by AT, instead of the pure OAM spatial modes, are used as information carriers, and the overlapping between the deformed OAM spatial modes are computed as the correlation coefficients between the users. Then, we present a turbulence mitigation scheme based on MUD idea to enhance AT tolerance of the OAM-multiplexed FSO communication link. In the proposed scheme, the crosstalk caused by AT is used as a useful component to deduce users’ information. The numerical results show that the performance of the OAM-multiplexed communication link has greatly improved by the proposed scheme. When the turbulence strength is 1 × 10-15 m-2/3, the transmission distance is 1000 m and the channel signal-to-noise ratio (SNR) is 26 dB, the bit-error-rate (BER) performance of four spatial multiplexed OAM modes lm = +1,+2,+3,+4 are all close to 10-5, and there is a 2-3 fold increase in the BER performance in comparison with those results without the proposed scheme. In addition, the proposed scheme is more effective for an OAM-multiplexed FSO communication link with a larger OAM mode topological charge interval. The proposed scheme is a promising direction for compensating the interference caused by AT in the OAM-multiplexed FSO communication link. Project supported by the National Natural Science Foundation of China (Grant Nos. 61271238 and 61475075), the Open Research Fund of Key Lab of Broadband Wireless Communication and Sensor Network Technology, Ministry of Education, China (Grant No. NYKL2015011), the Postgraduate Innovation Research Plan of Jiangsu Province, China (Grant No. CXZZ13_0489), and the University Natural Science Foundation of Jiangsu Province, China (Grant No. 16KJB510037).

Max-margin weight learning for medical knowledge network.

PubMed

Jiang, Jingchi; Xie, Jing; Zhao, Chao; Su, Jia; Guan, Yi; Yu, Qiubin

2018-03-01

The application of medical knowledge strongly affects the performance of intelligent diagnosis, and method of learning the weights of medical knowledge plays a substantial role in probabilistic graphical models (PGMs). The purpose of this study is to investigate a discriminative weight-learning method based on a medical knowledge network (MKN). We propose a training model called the maximum margin medical knowledge network (M 3 KN), which is strictly derived for calculating the weight of medical knowledge. Using the definition of a reasonable margin, the weight learning can be transformed into a margin optimization problem. To solve the optimization problem, we adopt a sequential minimal optimization (SMO) algorithm and the clique property of a Markov network. Ultimately, M 3 KN not only incorporates the inference ability of PGMs but also deals with high-dimensional logic knowledge. The experimental results indicate that M 3 KN obtains a higher F-measure score than the maximum likelihood learning algorithm of MKN for both Chinese Electronic Medical Records (CEMRs) and Blood Examination Records (BERs). Furthermore, the proposed approach is obviously superior to some classical machine learning algorithms for medical diagnosis. To adequately manifest the importance of domain knowledge, we numerically verify that the diagnostic accuracy of M 3 KN is gradually improved as the number of learned CEMRs increase, which contain important medical knowledge. Our experimental results show that the proposed method performs reliably for learning the weights of medical knowledge. M 3 KN outperforms other existing methods by achieving an F-measure of 0.731 for CEMRs and 0.4538 for BERs. This further illustrates that M 3 KN can facilitate the investigations of intelligent healthcare. Copyright © 2018 Elsevier B.V. All rights reserved.

Flexible and scalable wavelength multicast of coherent optical OFDM with tolerance against pump phase-noise using reconfigurable coherent multi-carrier pumping.

PubMed

Lu, Guo-Wei; Bo, Tianwai; Sakamoto, Takahide; Yamamoto, Naokatsu; Chan, Calvin Chun-Kit

2016-10-03

Recently the ever-growing demand for dynamic and high-capacity services in optical networks has resulted in new challenges that require improved network agility and flexibility in order for network resources to become more "consumable" and dynamic, or elastic, in response to requests from higher network layers. Flexible and scalable wavelength conversion or multicast is one of the most important technologies needed for developing agility in the physical layer. This paper will investigate how, using a reconfigurable coherent multi-carrier as a pump, the multicast scalability and the flexibility in wavelength allocation of the converted signals can be effectively improved. Moreover, the coherence in the multiple carriers prevents the phase noise transformation from the local pump to the converted signals, which is imperative for the phase-noise-sensitive multi-level single- or multi-carrier modulated signal. To verify the feasibility of the proposed scheme, we experimentally demonstrate the wavelength multicast of coherent optical orthogonal frequency division multiplexing (CO-OFDM) signals using a reconfigurable coherent multi-carrier pump, showing flexibility in wavelength allocation, scalability in multicast, and tolerance against pump phase noise. Less than 0.5 dB and 1.8 dB power penalties at a bit-error rate (BER) of 10^-3 are obtained for the converted CO-OFDM-quadrature phase-shift keying (QPSK) and CO-OFDM-16-ary quadrature amplitude modulation (16QAM) signals, respectively, even when using a distributed feedback laser (DFB) as a pump source. In contrast, with a free-running pumping scheme, the phase noise from DFB pumps severely deteriorates the CO-OFDM signals, resulting in a visible error-floor at a BER of 10^-2 in the converted CO-OFDM-16QAM signals.

Advanced time and wavelength division multiplexing for metropolitan area optical data communication networks

NASA Astrophysics Data System (ADS)

Watford, M.; DeCusatis, C.

2005-09-01

With the advent of new regulations governing the protection and recovery of sensitive business data, including the Sarbanes-Oxley Act, there has been a renewed interest in business continuity and disaster recovery applications for metropolitan area networks. Specifically, there has been a need for more efficient bandwidth utilization and lower cost per channel to facilitate mirroring of multi-terabit data bases. These applications have further blurred the boundary between metropolitan and wide area networks, with synchronous disaster recovery applications running up to 100 km and asynchronous solutions extending to 300 km or more. In this paper, we discuss recent enhancements in the Nortel Optical Metro 5200 Dense Wavelength Division Multiplexing (DWDM) platform, including features recently qualified for data communication applications such as Metro Mirror, Global Mirror, and Geographically Distributed Parallel Sysplex (GDPS). Using a 10 Gigabit/second (Gbit/s) backbone, this solution transports significantly more Fibre Channel protocol traffic with up to five times greater hardware density in the same physical package. This is also among the first platforms to utilize forward error correction (FEC) on the aggregate signals to improve bit error rate (BER) performance beyond industry standards. When combined with encapsulation into wide area network protocols, the use of FEC can compensate for impairments in BER across a service provider infrastructure without impacting application level performance. Design and implementation of these features will be discussed, including results from experimental test beds which validate these solutions for a number of applications. Future extensions of this environment will also be considered, including ways to provide configurable bandwidth on demand, mitigate Fibre Channel buffer credit management issues, and support for other GDPS protocols.

Base Excision Repair of Tandem Modifications in a Methylated CpG Dinucleotide*

PubMed Central

Sassa, Akira; Çağlayan, Melike; Dyrkheeva, Nadezhda S.; Beard, William A.; Wilson, Samuel H.

2014-01-01

Cytosine methylation and demethylation in tracks of CpG dinucleotides is an epigenetic mechanism for control of gene expression. The initial step in the demethylation process can be deamination of 5-methylcytosine producing the TpG alteration and T:G mispair, and this step is followed by thymine DNA glycosylase (TDG) initiated base excision repair (BER). A further consideration is that guanine in the CpG dinucleotide may become oxidized to 7,8-dihydro-8-oxoguanine (8-oxoG), and this could affect the demethylation process involving TDG-initiated BER. However, little is known about the enzymology of BER of altered in-tandem CpG dinucleotides; e.g. Tp8-oxoG. Here, we investigated interactions between this altered dinucleotide and purified BER enzymes, the DNA glycosylases TDG and 8-oxoG DNA glycosylase 1 (OGG1), apurinic/apyrimidinic (AP) endonuclease 1, DNA polymerase β, and DNA ligases. The overall TDG-initiated BER of the Tp8-oxoG dinucleotide is significantly reduced. Specifically, TDG and DNA ligase activities are reduced by a 3′-flanking 8-oxoG. In contrast, the OGG1-initiated BER pathway is blocked due to the 5′-flanking T:G mispair; this reduces OGG1, AP endonuclease 1, and DNA polymerase β activities. Furthermore, in TDG-initiated BER, TDG remains bound to its product AP site blocking OGG1 access to the adjacent 8-oxoG. These results reveal BER enzyme specificities enabling suppression of OGG1-initiated BER and coordination of TDG-initiated BER at this tandem alteration in the CpG dinucleotide. PMID:24695738

[Feasibility of 3BER-S Process for the Deep Denitrification in Synch with the Removal of PAEs from Reclaimed Water].

PubMed

Xu, Peng-cheng; Hao, Rui-xia; Zhang, Ya; Wang, Dong-yue; Zhong, Li-yan; Xu, Hao-dan

2016-02-15

In order to investigate the feasibility of deep denitrification and simultaneous removing phthalate esters (PAEs) in the process of reclaimed water treatment uses three-dimensional biofilm-electrode reactor coupled with sulfur autotrophic deep denitrification technology (3BER-S), the technological characteristics and mechanisms were analyzed based on determining the static adsorption capacity of biofilm cultured active carbon fillers in 3BER-S reactor together with the operation results of dynamic denitrification and simultaneous PAEs removing. The results showed that the average adsorption rates of DBP, DEHP were 85.84% and 97.12% in the biofilm cultured active carbon fillers, the equilibrium adsorption capacities were 0.1426 mg x g(-1) and 0.162 mg(-1) and the time spans of reaching adsorption saturation were 120 min and 60 min, respectively; The existence of PAEs had no obvious effect on denitrification, the reactor effluent concentration of TN was in range of 1-2 mg x L(-1) before and after the addition of PAEs, and the average removal rate of TN reached above 94%; 3BER-S denitrification system showed significant ability in removing PAEs, leading to effluent concentrations of DBP and DEHP of no more than 6 microg x L(-1) with removal rates of above 96%; this was due to the synergistic effect of absorption, biodegradation and electrochemistry. After treatment with 3BER-S technology, DBP and DEHP in simulative municipal secondary effluent met the regulated limitation of The Reuse of Urban Recycling Water Quality Standard for Groundwater Recharge (GB/T 19772-2005).

Anti-hepatocarcinoma effects of berberine-nanostructured lipid carriers against human HepG2, Huh7, and EC9706 cancer cell lines

NASA Astrophysics Data System (ADS)

Meng, Xiang-Ping; Fan, Hua; Wang, Yi-fei; Wang, Zhi-ping; Chen, Tong-sheng

2016-10-01

Hepatocarcinoma and esophageal squamous cell carcinomas threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma and esophageal carcinoma to chemotherapy. Berberine (Ber), an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma and antiesophageal carcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Ber loaded nanostructured lipid carriers (Ber-NLC) was prepared by hot melting and then high pressure homogenization technique. The in vitro anti-hepatocarcinoma and antiesophageal carcinoma effects of Ber-NLC relative to efficacy of bulk Ber were evaluated. The particle size and zeta potential of Ber-NLC were 189.3 +/- 3.7 nm and -19.3 +/- 1.4 mV, respectively. MTT assay showed that Ber-NLC effectively inhibited the proliferation of human HepG2 and Huh7 and EC9706 cells, and the corresponding IC50 value was 9.1 μg/ml, 4.4 μg/ml, and 6.3 μg/ml (18.3μg/ml, 6.5μg/ml, and 12.4μg/ml μg/ml of bulk Ber solution), respectively. These results suggest that the delivery of Ber-NLC is a promising approach for treating tumors.

In vitro antitumor efficacy of berberine: solid lipid nanoparticles against human HepG2, Huh7 and EC9706 cancer cell lines

NASA Astrophysics Data System (ADS)

Meng, Xiang-Ping; Wang, Xiao; Wang, Huai-ling; Chen, Tong-sheng; Wang, Yi-fei; Wang, Zhi-ping

2016-03-01

Hepatocarcinoma and esophageal squamous cell carcinomas threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma and esophageal carcinoma to chemotherapy. Berberine (Ber), an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma and antiesophageal carcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Ber loaded solid lipid nanoparticles (Ber-SLN) was prepared by hot melting and then high pressure homogenization technique. The in vitro anti-hepatocarcinoma and antiesophageal carcinoma effects of Ber-SLN relative to efficacy of bulk Ber were evaluated. The particle size and zeta potential of Ber-SLN were 154.3 ± 4.1 nm and -11.7 ± 1.8 mV, respectively. MTT assay showed that Ber-SLN effectively inhibited the proliferation of human HepG2 and Huh7 and EC9706 cells, and the corresponding IC50 value was 10.6 μg/ml, 5.1 μg/ml, and 7.3 μg/ml (18.3μg/ml, 6.5μg/ml, and 12.4μg/ml μg/ml of bulk Ber solution), respectively. These results suggest that the delivery of Ber-SLN is a promising approach for treating tumors.

Science and craftsmanship: the art of experiment and instrument making.

PubMed

Dierig, Sven

2006-01-01

In his two-volume monograph Untersuchungen über thierische Elektricität, the Berlin physiologist Emil du Bois-Reymond described the relation between nervous electricity and muscle mechanics by way of a long series of experiments. This work is a key text in the history of the experimental life sciences. But it not only contains new findings about the functioning of muscles and its nerves. Du Bois-Reymond practiced an art of experimentation in which aesthetics of mechanical craftsmanship allied itself with the science of physiology. Experimentation, as du Bois-Reymond understood it, was simultaneously an epistemic and an aesthetic practice. The goal of his science was thus producing both knowledge and aesthetic success. To cite this article: S. Dierig, C. R. Biologies 329 (2006).

Effect of non-Kolmogorov turbulence on BER performance in uplink ground-to-satellite laser communication

NASA Astrophysics Data System (ADS)

Wang, Yi; Zhang, Ao; Ma, Jing; Guo, Qiang

2016-12-01

Based on the non-Kolmogorov turbulence model of the stratosphere, a theoretical model for the influence of the angle of arrival (AOA) fluctuations on the performance of the bit error rate (BER) is established. A closed form expression of BER is then derived based on this model. Then, considering the combined effect of scintillation, beam wander and AOA fluctuations, the probability density function of the received intensity and the closed form expressions of BER for uplink are derived. Coherent detection of Circle Polarization Shift Keying modulation which is suitable for ground-to-satellite laser communication is employed. For an uplink, the BER performance is emulation analyzed and compared to the condition without taking AOA fluctuations into account. And the variation of BER as a function of AOA fluctuations has also been researched. The influence of intensity scintillation, beam wander and AOA fluctuations on BER has been analyzed based on laser transmit power restrictions.

Crisis-Expectant Planning for Crisis Relocation.

DTIC Science & Technology

1981-10-01

WORK UNIT NUmBERS Human Sciences Research, Inc. 7710 Old Springhouse Road Work Unit 482 1G McLean, Virginia 22102 It. CONTROLLING OFFICE NAME AND... Controlling Office) UNCLASSUIFIEDS.(1 ereot I Sm. DECLASSIFICATION/DOWNGRADING SCHEDULE 16. DISTRIBUTION STATE64ENT (of &hs .1. oer) Approved for Pttblic...preattack relocation period. 4. Improved population control with respect to orchestrating or phasing the evacuation movement, the post-evacuation

Demonstration of hybrid orbital angular momentum multiplexing and time-division multiplexing passive optical network.

PubMed

Wang, Andong; Zhu, Long; Liu, Jun; Du, Cheng; Mo, Qi; Wang, Jian

2015-11-16

Mode-division multiplexing passive optical network (MDM-PON) is a promising scheme for next-generation access networks to further increase fiber transmission capacity. In this paper, we demonstrate the proof-of-concept experiment of hybrid mode-division multiplexing (MDM) and time-division multiplexing (TDM) PON architecture by exploiting orbital angular momentum (OAM) modes. Bidirectional transmissions with 2.5-Gbaud 4-level pulse amplitude modulation (PAM-4) downstream and 2-Gbaud on-off keying (OOK) upstream are demonstrated in the experiment. The observed optical signal-to-noise ratio (OSNR) penalties for downstream and upstream transmissions at a bit-error rate (BER) of 2 × 10(-3) are less than 2.0 dB and 3.0 dB, respectively.

DEADS: Depth and Energy Aware Dominating Set Based Algorithm for Cooperative Routing along with Sink Mobility in Underwater WSNs.

PubMed

Umar, Amara; Javaid, Nadeem; Ahmad, Ashfaq; Khan, Zahoor Ali; Qasim, Umar; Alrajeh, Nabil; Hayat, Amir

2015-06-18

Performance enhancement of Underwater Wireless Sensor Networks (UWSNs) in terms of throughput maximization, energy conservation and Bit Error Rate (BER) minimization is a potential research area. However, limited available bandwidth, high propagation delay, highly dynamic network topology, and high error probability leads to performance degradation in these networks. In this regard, many cooperative communication protocols have been developed that either investigate the physical layer or the Medium Access Control (MAC) layer, however, the network layer is still unexplored. More specifically, cooperative routing has not yet been jointly considered with sink mobility. Therefore, this paper aims to enhance the network reliability and efficiency via dominating set based cooperative routing and sink mobility. The proposed work is validated via simulations which show relatively improved performance of our proposed work in terms the selected performance metrics.

Base Excision Repair Facilitates a Functional Relationship Between Guanine Oxidation and Histone Demethylation

PubMed Central

Li, Jianfeng; Braganza, Andrea

2013-01-01

Abstract Significance: Appropriately controlled epigenetic regulation is critical for the normal development and health of an organism. Misregulation of epigenetic control via deoxyribonucleic acid (DNA) methylation or histone methylation has been associated with cancer and chromosomal instability syndromes. Recent Advances: The main function of the proteins in the base excision repair (BER) pathway is to repair DNA single-strand breaks and deamination, oxidation, and alkylation-induced DNA base damage that may result from chemotherapy, environmental exposure, or byproducts of cellular metabolism. Recent studies have suggested that one or more BER proteins may also participate in epigenetic regulation to facilitate gene expression modulation via alteration of the state of DNA methylation or via a reaction coupled to histone modification. BER proteins have also been reported to play an essential role in pluripotent stem cell reprogramming. Critical Issues: One emerging function for BER in epigenetic regulation is the repair of base lesions induced by hydrogen peroxide as a byproduct of lysine-specific demethylase 1 (LSD1) enzymatic activity (LSD1/LSD2-coupled BER) for transcriptional regulation. Future Directions: To shed light on this novel role of BER, this review focuses on the repair of oxidative lesions in nuclear DNA that are induced during LSD1-mediated histone demethylation. Further, we highlight current studies suggesting a role for BER proteins in transcriptional regulation of gene expression via BER-coupled active DNA demethylation in mammalian cells. Such efforts to address the role of BER proteins in epigenetic regulation could broaden cancer therapeutic strategies to include epigenetic modifiers combined with BER inhibitors. Antioxid. Redox Signal. 18, 2429–2443. PMID:23311711

N-methylpurine DNA glycosylase and DNA polymerase β modulate BER inhibitor potentiation of glioma cells to temozolomide

PubMed Central

Tang, Jiang-bo; Svilar, David; Trivedi, Ram N.; Wang, Xiao-hong; Goellner, Eva M.; Moore, Briana; Hamilton, Ronald L.; Banze, Lauren A.; Brown, Ashley R.; Sobol, Robert W.

2011-01-01

Temozolomide (TMZ) is the preferred chemotherapeutic agent in the treatment of glioma following surgical resection and/or radiation. Resistance to TMZ is attributed to efficient repair and/or tolerance of TMZ-induced DNA lesions. The majority of the TMZ-induced DNA base adducts are repaired by the base excision repair (BER) pathway and therefore modulation of this pathway can enhance drug sensitivity. N-methylpurine DNA glycosylase (MPG) initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER. Using the human glioma cell lines LN428 and T98G, we report here that potentiation of TMZ via BER inhibition [methoxyamine (MX), the PARP inhibitors PJ34 and ABT-888 or depletion (knockdown) of PARG] is greatly enhanced by over-expression of the BER initiating enzyme MPG. We also show that methoxyamine-induced potentiation of TMZ in MPG expressing glioma cells is abrogated by elevated-expression of the rate-limiting BER enzyme DNA polymerase β (Polβ), suggesting that cells proficient for BER readily repair AP sites in the presence of MX. Further, depletion of Polβ increases PARP inhibitor-induced potentiation in the MPG over-expressing glioma cells, suggesting that expression of Polβ modulates the cytotoxic effect of combining increased repair initiation and BER inhibition. This study demonstrates that MPG overexpression, together with inhibition of BER, sensitizes glioma cells to the alkylating agent TMZ in a Polβ-dependent manner, suggesting that the expression level of both MPG and Polβ might be used to predict the effectiveness of MX and PARP-mediated potentiation of TMZ in cancer treatment. PMID:21377995

Performance Analysis of Receive Diversity in Wireless Sensor Networks over GBSBE Models

PubMed Central

Goel, Shivali; Abawajy, Jemal H.; Kim, Tai-hoon

2010-01-01

Wireless sensor networks have attracted a lot of attention recently. In this paper, we develop a channel model based on the elliptical model for multipath components involving randomly placed scatterers in the scattering region with sensors deployed on a field. We verify that in a sensor network, the use of receive diversity techniques improves the performance of the system. Extensive performance analysis of the system is carried out for both single and multiple antennas with the applied receive diversity techniques. Performance analyses based on variations in receiver height, maximum multipath delay and transmit power have been performed considering different numbers of antenna elements present in the receiver array, Our results show that increasing the number of antenna elements for a wireless sensor network does indeed improve the BER rates that can be obtained. PMID:22163510

Integration of FTTH and GI-POF in-house networks based on injection locking and direct-detection techniques.

PubMed

Peng, Hsiao-Chun; Lu, Hai-Han; Li, Chung-Yi; Su, Heng-Sheng; Hsu, Chin-Tai

2011-03-28

An integration of fiber-to-the-home (FTTH) and graded-index plastic optical fiber (GI-POF) in-house networks based on injection-locked vertical cavity surface emitting lasers (VCSELs) and direct-detection technique is proposed and experimentally demonstrated. Sufficient low bit error rate (BER) values were obtained over a combination of 20-km single-mode fiber (SMF) and 50-m GI-POF links. Signal qualities satisfy the worldwide interoperability for microwave access (WiMAX) requirement with data signals of 20 Mbps/5.8 GHz and 70 Mbps/10 GHz, respectively. Since our proposed network does not use sophisticated and expensive RF devices in premises, it reveals a prominent one with simpler and more economic advantages. Our proposed architecture is suitable for the SMF-based primary and GI-POF-based in-house networks.

Low threshold all-optical crossbar switch on GaAs-GaAlAs channel waveguide arrays

NASA Astrophysics Data System (ADS)

Jannson, Tomasz; Kostrzewski, Andrew

1994-09-01

During the Phase 2 project entitled 'Low Threshold All-Optical Crossbar Switch on GaAs - GaAlAs Channel Waveguide Array,' Physical Optics Corporation (POC) developed the basic principles for the fabrication of all-optical crossbar switches. Based on this development. POC fabricated a 2 x 2 GaAs/GaAlAs switch that changes the direction of incident light with minimum insertion loss and nonlinear distortion. This unique technology can be used in both analog and digital networks. The applications of this technology are widespread. Because the all-optical network does not have any speed limitations (RC time constant), POC's approach will be beneficial to SONET networks, phased array radar networks, very high speed oscilloscopes, all-optical networks, IR countermeasure systems, BER equipment, and the fast growing video conferencing network market. The novel all-optical crossbar switch developed in this program will solve interconnect problems. and will be a key component in the widely proposed all-optical 200 Gb/s SONET/ATM networks.

Strategic Industry Attack.

DTIC Science & Technology

1980-01-15

Code B364078464 V99QAXNH30303 H2590D. IS KEY WORDS fCo.. e.1 Odn Od It -C.eWV WHO Idnlif b 61-k n 0ber) Strategic Targeting Copper Industry INDATAK 20...develop, debug and test an industrial simulation model (INDATAK) using the LOGATAK model as a point of departure. The copper processing industry is...significant processes in the copper industry, including the transportation network connecting the processing elements, have been formatted for use in

A unified view of base excision repair: lesion-dependent protein complexes regulated by post-translational modification

PubMed Central

Almeida, Karen H.; Sobol, Robert W.

2007-01-01

Base excision repair (BER) proteins act upon a significantly broad spectrum of DNA lesions that result from endogenous and exogenous sources. Multiple sub-pathways of BER (short-path or long-patch) and newly designated DNA repair pathways (e.g., SSBR and NIR) that utilize BER proteins complicate any comprehensive understanding of BER and its role in genome maintenance, chemotherapeutic response, neurodegeneration, cancer or aging. Herein, we propose a unified model of BER, comprised of three functional processes: Lesion Recognition/Strand Scission, Gap Tailoring and DNA Synthesis/Ligation, each represented by one or more multiprotein complexes and coordinated via the XRCC1/DNA Ligase III and PARP1 scaffold proteins. BER therefore may be represented by a series of repair complexes that assemble at the site of the DNA lesion and mediates repair in a coordinated fashion involving protein-protein interactions that dictate subsequent steps or sub-pathway choice. Complex formation is influenced by post-translational protein modifications that arise from the cellular state or the DNA damage response, providing an increase in specificity and efficiency to the BER pathway. In this review, we have summarized the reported BER protein-protein interactions and protein post-translational modifications and discuss the impact on DNA repair capacity and complex formation. PMID:17337257

Noise tolerance in optical waveguide circuits for recognition of optical 16 quadrature amplitude modulation codes

NASA Astrophysics Data System (ADS)

Inoshita, Kensuke; Hama, Yoshimitsu; Kishikawa, Hiroki; Goto, Nobuo

2016-12-01

In photonic label routers, various optical signal processing functions are required; these include optical label extraction, recognition of the label, optical switching and buffering controlled by signals based on the label information and network routing tables, and label rewriting. Among these functions, we focus on photonic label recognition. We have proposed two kinds of optical waveguide circuits to recognize 16 quadrature amplitude modulation codes, i.e., recognition from the minimum output port and from the maximum output port. The recognition function was theoretically analyzed and numerically simulated by finite-difference beam-propagation method. We discuss noise tolerance in the circuit and show numerically simulated results to evaluate bit-error-rate (BER) characteristics against optical signal-to-noise ratio (OSNR). The OSNR required to obtain a BER less than 1.0×10-3 for the symbol rate of 2.5 GBaud was 14.5 and 27.0 dB for recognition from the minimum and maximum output, respectively.

The effect of total noise on two-dimension OCDMA codes

NASA Astrophysics Data System (ADS)

Dulaimi, Layth A. Khalil Al; Badlishah Ahmed, R.; Yaakob, Naimah; Aljunid, Syed A.; Matem, Rima

2017-11-01

In this research, we evaluate the performance of total noise effect on two dimension (2-D) optical code-division multiple access (OCDMA) performance systems using 2-D Modified Double Weight MDW under various link parameters. The impact of the multi-access interference (MAI) and other noise effect on the system performance. The 2-D MDW is compared mathematically with other codes which use similar techniques. We analyzed and optimized the data rate and effective receive power. The performance and optimization of MDW code in OCDMA system are reported, the bit error rate (BER) can be significantly improved when the 2-D MDW code desired parameters are selected especially the cross correlation properties. It reduces the MAI in the system compensate BER and phase-induced intensity noise (PIIN) in incoherent OCDMA The analysis permits a thorough understanding of PIIN, shot and thermal noises impact on 2-D MDW OCDMA system performance. PIIN is the main noise factor in the OCDMA network.

Biological and Environmental Research Program at Oak Ridge National Laboratory, FY 1992--1994

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This report is the 1992--1994 Program Director's Overview Report for Oak Ridge National Laboratory's (ORNL's) Biological and Environmental Research (BER) Program, and as such it addresses KP-funded work at ORNL conducted during FY 1991 and in progress during FY 1992; it also serves as a planning document for the remainder of FY 1992 through FY 1994. Non-BER funded work at ORNL relevant to the mission of OHER is also discussed. The second section of the report describes ORNL facilities and resources used by the BER program. The third section addresses research management practices at ORNL. The fourth, fifth, and sixthmore » sections address BER-funded research in progress, program accomplishments and research highlights, and program orientation for the remainder of FY 1992 through FY 1994, respectively. Work for non-BER sponsors is described in the seventh section, followed by a discussion of significant near and long-term issues facing BER work at ORNL in the eighth section. The last section provides a statistical summary of BER research at ORNL. Appendices supplement the above topics with additional detail.« less

Morphological and immunohistochemical characterization of isolated tumor cells by p53 status in gastrointestinal tumors.

PubMed

Milsmann, C; Füzesi, L; Heinmöller, E; Krause, P; Werner, C; Becker, H; Horstmann, O

2008-01-01

Isolated tumor cells (ITCs) in cancer patients are retrieved mostly using immunohistochemistry with antibodies directed against antiepithelial antigens (for example Ber-EP4), which are supposed not to be present in metastatic-free tissue. To date, there has been ongoing controversy whether those cells have biologic significance and are linked with tumor progression and impaired patient's prognosis. Therefore, the aim of this study was to further characterize Ber-EP4-positive cells in various tissues, with special emphasis on their tumorigenic origin. The frequency and prognostic impact of ITCs in lymph nodes displayed by means of monoclonal antibody Ber-EP4 were evaluated in retrospective (n = 292) and prospective (n = 100) collectives of various gastrointestinal carcinomas free of metastatic disease in conventional histopathology (pN0). Furthermore, the frequency of ITCs in the peritoneal cavity and bone marrow was analyzed in case of absence of overt distant metastasis (pM0) in the prospective collective. Ber-EP4-immunoreactive cells were further characterized for tumorigenic origin using morphological criteria and immunohistochemical double staining for Ber-EP4 and p53. Ber-EP4-positive cells could be revealed in lymph nodes in 44.3% of pN0-gastrointestinal carcinomas, in the peritoneal cavity in 19%, and in the bone marrow in 10%. In lymph nodes, BerEP4-immunoreactive cells exhibited a metastatic-atypical morphology in 59%; however, it was always typical for true tumor cells in the peritoneal cavity or bone marrow. The cumulative 5-year survival rate was adversely affected by Ber-EP4-immunoreactive cells in uni- and multivariate analysis, irrespective of the underlying cell morphology (68% for Ber-EP4 negative, 41% for Ber-EP4 positive with atypical and typical morphology each). In the case of a p53-positive primary tumor, 70% of the corresponding ITCs also overexpressed p53, while the remainder was deemed p53 negative (p = 0.002). ITCs detected by the antiepithelial antibody Ber-EP4 are present in a substantial proportion of apparently tumor-free lymph nodes. These cells impair patients' prognoses, irrespective of the underlying cell morphology. As approximately one third of Ber-EP4-positive cells in p53-positive primary tumors do not overexpress p53; their true tumorigenic origin needs to be further investigated.

Epistatic role of base excision repair and mismatch repair pathways in mediating cisplatin cytotoxicity

PubMed Central

Kothandapani, Anbarasi; Sawant, Akshada; Dangeti, Venkata Srinivas Mohan Nimai; Sobol, Robert W.; Patrick, Steve M.

2013-01-01

Base excision repair (BER) and mismatch repair (MMR) pathways play an important role in modulating cis-Diamminedichloroplatinum (II) (cisplatin) cytotoxicity. In this article, we identified a novel mechanistic role of both BER and MMR pathways in mediating cellular responses to cisplatin treatment. Cells defective in BER or MMR display a cisplatin-resistant phenotype. Targeting both BER and MMR pathways resulted in no additional resistance to cisplatin, suggesting that BER and MMR play epistatic roles in mediating cisplatin cytotoxicity. Using a DNA Polymerase β (Polβ) variant deficient in polymerase activity (D256A), we demonstrate that MMR acts downstream of BER and is dependent on the polymerase activity of Polβ in mediating cisplatin cytotoxicity. MSH2 preferentially binds a cisplatin interstrand cross-link (ICL) DNA substrate containing a mismatch compared with a cisplatin ICL substrate without a mismatch, suggesting a novel mutagenic role of Polβ in activating MMR in response to cisplatin. Collectively, these results provide the first mechanistic model for BER and MMR functioning within the same pathway to mediate cisplatin sensitivity via non-productive ICL processing. In this model, MMR participation in non-productive cisplatin ICL processing is downstream of BER processing and dependent on Polβ misincorporation at cisplatin ICL sites, which results in persistent cisplatin ICLs and sensitivity to cisplatin. PMID:23761438

FPGA implementation of advanced FEC schemes for intelligent aggregation networks

NASA Astrophysics Data System (ADS)

Zou, Ding; Djordjevic, Ivan B.

2016-02-01

In state-of-the-art fiber-optics communication systems the fixed forward error correction (FEC) and constellation size are employed. While it is important to closely approach the Shannon limit by using turbo product codes (TPC) and low-density parity-check (LDPC) codes with soft-decision decoding (SDD) algorithm; rate-adaptive techniques, which enable increased information rates over short links and reliable transmission over long links, are likely to become more important with ever-increasing network traffic demands. In this invited paper, we describe a rate adaptive non-binary LDPC coding technique, and demonstrate its flexibility and good performance exhibiting no error floor at BER down to 10-15 in entire code rate range, by FPGA-based emulation, making it a viable solution in the next-generation high-speed intelligent aggregation networks.

High-Data-Rate Quadrax Cable Microwave Characterization at the NASA Glenn Structural Dynamics Laboratory

NASA Technical Reports Server (NTRS)

Theofylaktos, Onoufrios; Warner, Joseph D.; Sheehe, Charles J.

2012-01-01

An experiment was performed to determine the degradation in the bit-error-rate (BER) in the high-data-rate cables chosen for the Orion Service Module due to extreme launch conditions of vibrations with a magnitude of 60g. The cable type chosen for the Orion Service Module was no. 8 quadrax cable. The increase in electrical noise induced on these no. 8 quadrax cables was measured at the NASA Glenn vibration facility in the Structural Dynamics Laboratory. The intensity of the vibrations was set at 32g, which was the maximum available level at the facility. The cable lengths used during measurements were 1, 4, and 8 m. The noise measurements were done in an analog fashion using a performance network analyzer (PNA) by recording the standard deviation of the transmission scattering parameter S(sub 21) over the frequency range of 100 to 900 MHz. The standard deviation of S(sub 210 was measured before, during, and after the vibration of the cables at the vibration facility. We observed an increase in noise by a factor of 2 to 6. From these measurements we estimated the increase expected in the BER for a cable length of 25 m and concluded that these findings are large enough that the noise increase due to vibration must be taken in to account for the design of the communication system for a BER of 10(exp -8).

Uplink transmission of a 60-km-reach WDM/OCDM-PON using a spectrum-sliced pulse source

NASA Astrophysics Data System (ADS)

Choi, Yong-Kyu; Hanawa, Masanori; Park, Chang-Soo

2014-02-01

We propose and experimentally demonstrate the uplink transmission of a 60-km-reach wavelength division multiplexing/optical code division multiplexing (WDM/OCDM) passive optical network (PON) using a spectrum-sliced pulse source. As a single light source, a broadband pulse source with a bandwidth of 6.5 nm and a repetition rate of 1.25 GHz is generated at a central office and supplied to a remote node (RN) through a 50-km fiber link. At the RN, narrow-band pulses (as a source for uplink transmission) are obtained by spectrum slicing the broadband pulse source with a cyclic arrayed waveguide grating and are then supplied to all optical network units (ONUs) via 1×4 power splitters and 10-km drop fibers. Eight wavelengths are obtained with a 6.5-nm bandwidth of the broadband pulse source, and the qualities of the pulses with a repetition rate of 1.25 GHz and a pulse width of 45 ps for the eight wavelengths are sufficient for four-chip OCDM encoding at the ONUs. In our experiments, four signals are multiplexed by OCDM at one wavelength, and another encoded signal is also multiplexed by WDM. The bit error rates (BERs) of the signals exhibit error-free transmission (BER<10-9) over a 60-km single-mode fiber at 1.25 Gb/s.

Performance Comparison between CDTD and STTD for DS-CDMA/MMSE-FDE with Frequency-Domain ICI Cancellation

NASA Astrophysics Data System (ADS)

Takeda, Kazuaki; Kojima, Yohei; Adachi, Fumiyuki

Frequency-domain equalization (FDE) based on the minimum mean square error (MMSE) criterion can provide a better bit error rate (BER) performance than rake combining. However, the residual inter-chip interference (ICI) is produced after MMSE-FDE and this degrades the BER performance. Recently, we showed that frequency-domain ICI cancellation can bring the BER performance close to the theoretical lower bound. To further improve the BER performance, transmit antenna diversity technique is effective. Cyclic delay transmit diversity (CDTD) can increase the number of equivalent paths and hence achieve a large frequency diversity gain. Space-time transmit diversity (STTD) can obtain antenna diversity gain due to the space-time coding and achieve a better BER performance than CDTD. Objective of this paper is to show that the BER performance degradation of CDTD is mainly due to the residual ICI and that the introduction of ICI cancellation gives almost the same BER performance as STTD. This study provides a very important result that CDTD has a great advantage of providing a higher throughput than STTD. This is confirmed by computer simulation. The computer simulation results show that CDTD can achieve higher throughput than STTD when ICI cancellation is introduced.

TWC and AWG based optical switching structure for OVPN in WDM-PON

NASA Astrophysics Data System (ADS)

Bai, Hui-feng; Chen, Yu-xin; Wang, Qin

2015-03-01

With the rapid development of optical elements with large capacity and high speed, the network architecture is of great importance in determing the performance of wavelength division multiplexing passive optical network (WDM-PON). This paper proposes a switching structure based on the tunable wavelength converter (TWC) and the arrayed-waveguide grating (AWG) for WDM-PON, in order to provide the function of opitcal virtual private network (OVPN). Using the tunable wavelength converter technology, this switch structure is designed and works between the optical line terminal (OLT) and optical network units (ONUs) in the WDM-PON system. Moreover, the wavelength assignment of upstream/downstream can be realized and direct communication between ONUs is also allowed by privite wavelength channel. Simulation results show that the proposed TWC and AWG based switching structure is able to achieve OVPN function and to gain better performances in terms of bite error rate (BER) and time delay.

Impact of nonlinearity phenomenon FWM in DWDM optical link considering dispersive fiber

NASA Astrophysics Data System (ADS)

Puche, William S.; Amaya, Ferney O.; Sierra, Javier E.

2013-12-01

The increasing demand of network traffic requires new research centers; improve their communications networks, due to the excessive use of mobile and portable devices wanting to have greater access to the network by downloading interactive content quickly and effectively. For our case analyze optical network link through simulation results assuming a DWDM (Dense wavelength Division Multiplexing) optical link, considering the nonlinearity phenomenon FWM (Four Mixed Wavelength) in order to compare their performance, assuming transmission bit rates to 2.5 Gbps and 10 Gbps, using three primary wavelengths of 1450 nm, 1550 nm and 1650 nm for the transmission of information, whose separation is 100 GHz to generate 16 channels or user information. Tests were conducted to analyze optical amplifiers EDFAs link robustness at a maximum distance of 200 km and identify parameters OSNR, SNR and BER, for a robust and effective transmission

Deep Drilling Results in the Atlantic Ocean: Continental Margins and Paleoenvironment

DTIC Science & Technology

1979-01-01

nannoplankton, a sparse to rich DITIBTO OF HORIZO C I foraminiferal fauna (simple arenaceous foramin - 5S SSftB / ifera, lagenids, epistominids, and primitive...Deep Sea Pessagno, E.A., Jr., Mesozoic Planctonic Foramin - DrillingP , 11, Washington (U.S. Govern- vera and Radiolaria, in Ewing, M., Worzel, L.J. ment...Strati- B.,er, W.H., Foramin ooze: solution at graphic Micropaleontology of Atlantic Basins depths, Science, 156, 383-385, 1967. and Borderlands

Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication.

PubMed

Bj Rås, Karine Ø; Sousa, Mirta M L; Sharma, Animesh; Fonseca, Davi M; S Gaard, Caroline K; Bj Rås, Magnar; Otterlei, Marit

2017-08-21

Base lesions in DNA can stall the replication machinery or induce mutations if bypassed. Consequently, lesions must be repaired before replication or in a post-replicative process to maintain genomic stability. Base excision repair (BER) is the main pathway for repair of base lesions and is known to be associated with DNA replication, but how BER is organized during replication is unclear. Here we coupled the iPOND (isolation of proteins on nascent DNA) technique with targeted mass-spectrometry analysis, which enabled us to detect all proteins required for BER on nascent DNA and to monitor their spatiotemporal orchestration at replication forks. We demonstrate that XRCC1 and other BER/single-strand break repair (SSBR) proteins are enriched in replisomes in unstressed cells, supporting a cellular capacity of post-replicative BER/SSBR. Importantly, we identify for the first time the DNA glycosylases MYH, UNG2, MPG, NTH1, NEIL1, 2 and 3 on nascent DNA. Our findings suggest that a broad spectrum of DNA base lesions are recognized and repaired by BER in a post-replicative process. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Base Excision Repair and Lesion-Dependent Subpathways for Repair of Oxidative DNA Damage

PubMed Central

Svilar, David; Goellner, Eva M.; Almeida, Karen H.

2011-01-01

Abstract Nuclear and mitochondrial genomes are under continuous assault by a combination of environmentally and endogenously derived reactive oxygen species, inducing the formation and accumulation of mutagenic, toxic, and/or genome-destabilizing DNA lesions. Failure to resolve these lesions through one or more DNA-repair processes is associated with genome instability, mitochondrial dysfunction, neurodegeneration, inflammation, aging, and cancer, emphasizing the importance of characterizing the pathways and proteins involved in the repair of oxidative DNA damage. This review focuses on the repair of oxidative damage–induced lesions in nuclear and mitochondrial DNA mediated by the base excision repair (BER) pathway in mammalian cells. We discuss the multiple BER subpathways that are initiated by one of 11 different DNA glycosylases of three subtypes: (a) bifunctional with an associated β-lyase activity; (b) monofunctional; and (c) bifunctional with an associated β,δ-lyase activity. These three subtypes of DNA glycosylases all initiate BER but yield different chemical intermediates and hence different BER complexes to complete repair. Additionally, we briefly summarize alternate repair events mediated by BER proteins and the role of BER in the repair of mitochondrial DNA damage induced by ROS. Finally, we discuss the relation of BER and oxidative DNA damage in the onset of human disease. Antioxid. Redox Signal. 14, 2491–2507. PMID:20649466

Chromatin associated mechanisms in base excision repair - nucleosome remodeling and DNA transcription, two key players.

PubMed

Menoni, Hervé; Di Mascio, Paolo; Cadet, Jean; Dimitrov, Stefan; Angelov, Dimitar

2017-06-01

Genomic DNA is prone to a large number of insults by a myriad of endogenous and exogenous agents. The base excision repair (BER) is the major mechanism used by cells for the removal of various DNA lesions spontaneously or environmentally induced and the maintenance of genome integrity. The presence of persistent DNA damage is not compatible with life, since abrogation of BER leads to early embryonic lethality in mice. There are several lines of evidences showing existence of a link between deficient BER, cancer proneness and ageing, thus illustrating the importance of this DNA repair pathway in human health. Although the enzymology of BER mechanisms has been largely elucidated using chemically defined DNA damage substrates and purified proteins, the complex interplay of BER with another vital process like transcription or when DNA is in its natural state (i.e. wrapped in nucleosome and assembled in chromatin fiber is largely unexplored. Cells use chromatin remodeling factors to overcome the general repression associated with the nucleosomal organization. It is broadly accepted that energy-dependent nucleosome remodeling factors disrupt histones-DNA interactions at the expense of ATP hydrolysis to favor transcription as well as DNA repair. Importantly, unlike transcription, BER is not part of a regulated developmental process but represents a maintenance system that should be efficient anytime and anywhere in the genome. In this review we will discuss how BER can deal with chromatin organization to maintain genetic information. Emphasis will be placed on the following challenging question: how BER is initiated within chromatin? Copyright © 2017 Elsevier Inc. All rights reserved.

Age and exposure to arsenic alter base excision repair transcript levels in mice.

PubMed

Osmond, Megan J; Kunz, Bernard A; Snow, Elizabeth T

2010-09-01

Arsenic (As) induces DNA-damaging reactive oxygen species. Most oxidative DNA damage is countered by base excision repair (BER), the capacity for which may be reduced in older animals. We examined whether age and consumption of As in lactational milk or drinking water influences BER gene transcript levels in mice. Lactating mothers and 24-week-old mice were exposed (24 h or 2 weeks) to As (2 or 50 p.p.m.) in drinking water. Lung tissue was harvested from adults, neonates (initially 1 week old) feeding from lactating mothers and untreated animals 1-26 weeks old. Transcripts encoding BER proteins were quantified. BER transcript levels decreased precipitously with age in untreated mice but increased in neonates whose mothers were exposed to 50 p.p.m. As for 24 h or 2 weeks. Treatment of 24-week-old mice with 2 or 50 p.p.m. As for 2 weeks decreased all transcript levels measured. Exposure to As attenuated the age-related transcript level decline for only one BER gene. We conclude that aging is associated with a rapid reduction of BER transcript levels in mice, which may contribute to decreased BER activity in older animals. Levels of As that can alter gene expression are transmitted to neonatal mice in lactational milk produced by mothers drinking water containing As, raising concerns about breastfeeding in countries having As-contaminated groundwater. Reduction of BER transcript levels in 24-week-old mice exposed to As for 2 weeks suggests As may potentiate sensitivity to itself in older animals.

Overcoming temozolomide resistance in glioblastoma via dual inhibition of NAD+ biosynthesis and base excision repair

PubMed Central

Goellner, Eva M.; Grimme, Bradford; Brown, Ashley R.; Lin, Ying-Chih; Wang, Xiao-Hong; Sugrue, Kelsey F.; Mitchell, Leah; Trivedi, Ram N.; Tang, Jiang-bo; Sobol, Robert W.

2011-01-01

Glioblastoma multiforme (GBM) is a devastating brain tumor with poor prognosis and low median survival time. Standard treatment includes radiation and chemotherapy with the DNA alkylating agent temozolomide (TMZ). However, a large percentage of tumors are resistant to the cytotoxic effects of the TMZ-induced DNA lesion O6-methylguanine (O6-MeG) due to elevated expression of the repair protein O6-methylguanine-DNA methyltransferase (MGMT) or a defect in the mismatch repair (MMR) pathway. Although a majority of the TMZ induced lesions (N7-methylguanine and N3-methyladenine) are base excision repair (BER) substrates, these DNA lesions are also readily repaired. However, blocking BER can enhance response to TMZ and therefore the BER pathway has emerged as an attractive target for reversing TMZ resistance. Our lab has recently reported that inhibition of BER leads to the accumulation of repair intermediates that induce energy depletion-mediated cell death via hyperactivation of poly(ADP-ribose) polymerase. Based on our observation that TMZ-induced cell death via BER inhibition is dependent on the availability of NAD+, we have hypothesized that combined BER and NAD+ biosynthesis inhibition will increase TMZ efficacy in glioblastoma cell lines greater than BER inhibition alone. Importantly, we find that the combination of BER and NAD+ biosynthesis inhibition significantly sensitizes glioma cells with elevated expression of MGMT and those deficient in MMR, two genotypes normally associated with TMZ resistance. Dual targeting of these two interacting pathways (DNA repair and NAD+ biosynthesis) may prove to be an effective treatment combination for patients with resistant and recurrent GBM. PMID:21406402

A Fully Implemented 12 × 12 Data Vortex Optical Packet Switching Interconnection Network

NASA Astrophysics Data System (ADS)

Shacham, Assaf; Small, Benjamin A.; Liboiron-Ladouceur, Odile; Bergman, Keren

2005-10-01

A fully functional optical packet switching (OPS) interconnection network based on the data vortex architecture is presented. The photonic switching fabric uniquely capitalizes on the enormous bandwidth advantage of wavelength division multiplexing (WDM) wavelength parallelism while delivering minimal packet transit latency. Utilizing semiconductor optical amplifier (SOA)-based switching nodes and conventional fiber-optic technology, the 12-port system exhibits a capacity of nearly 1 Tb/s. Optical packets containing an eight-wavelength WDM payload with 10 Gb/s per wavelength are routed successfully to all 12 ports while maintaining a bit error rate (BER) of 10-12 or better. Median port-to-port latencies of 110 ns are achieved with a distributed deflection routing network that resolves packet contention on-the-fly without the use of optical buffers and maintains the entire payload path in the optical domain.

Oxidized Base Damage and Single-Strand Break Repair in Mammalian Genomes: Role of Disordered Regions and Posttranslational Modifications in Early Enzymes

PubMed Central

Hegde, Muralidhar L.; Izumi, Tadahide; Mitra, Sankar

2012-01-01

Oxidative genome damage induced by reactive oxygen species includes oxidized bases, abasic (AP) sites, and single-strand breaks, all of which are repaired via the evolutionarily conserved base excision repair/single-strand break repair (BER/SSBR) pathway. BER/SSBR in mammalian cells is complex, with preferred and backup sub-pathways, and is linked to genome replication and transcription. The early BER/SSBR enzymes, namely, DNA glycosylases (DGs) and the end-processing proteins such as abasic endonuclease 1 (APE1), form complexes with downstream repair (and other noncanonical) proteins via pairwise interactions. Furthermore, a unique feature of mammalian early BER/ SSBR enzymes is the presence of a disordered terminal extension that is absent in their Escherichia coli prototypes. These nonconserved segments usually contain organelle-targeting signals, common interaction interfaces, and sites of posttranslational modifications that may be involved in regulating their repair function including lesion scanning. Finally, the linkage of BER/SSBR deficiency to cancer, aging, and human neurodegenerative diseases, and therapeutic targeting of BER/SSBR are discussed. PMID:22749145

On the optimum signal constellation design for high-speed optical transport networks.

PubMed

Liu, Tao; Djordjevic, Ivan B

2012-08-27

In this paper, we first describe an optimum signal constellation design algorithm, which is optimum in MMSE-sense, called MMSE-OSCD, for channel capacity achieving source distribution. Secondly, we introduce a feedback channel capacity inspired optimum signal constellation design (FCC-OSCD) to further improve the performance of MMSE-OSCD, inspired by the fact that feedback channel capacity is higher than that of systems without feedback. The constellations obtained by FCC-OSCD are, however, OSNR dependent. The optimization is jointly performed together with regular quasi-cyclic low-density parity-check (LDPC) code design. Such obtained coded-modulation scheme, in combination with polarization-multiplexing, is suitable as both 400 Gb/s and multi-Tb/s optical transport enabling technology. Using large girth LDPC code, we demonstrate by Monte Carlo simulations that a 32-ary signal constellation, obtained by FCC-OSCD, outperforms previously proposed optimized 32-ary CIPQ signal constellation by 0.8 dB at BER of 10(-7). On the other hand, the LDPC-coded 16-ary FCC-OSCD outperforms 16-QAM by 1.15 dB at the same BER.

In-line monitoring technique with visible light from 1.3 microm-band SHG module for optical access systems.

PubMed

Kubo, Takahiro; Taniguchi, Tomohiro; Tadanaga, Osamu; Sakurai, Naoya; Kimura, Hideaki; Hadama, Hisaya; Asobe, Masaki

2010-02-01

We propose an in-line monitoring technique that uses 650 nm visible light for performing maintenance work on Fiber-to-the-home (FTTH) network quickly without the need for measuring skills or external devices. This technique is characterized by visible light (650 nm) generated by an SHG module from the 1.3 microm-band line signal. We fabricate a 1.3 microm-band quasi phase matched LiNbO(3) (QPM-LN) module, and the measure the 650 nm second harmonic (SH) power to test the proposed short-pulse modulation method. The results confirm the feasibility of the short-pulse modulation method with different peak factors (PFs) (1.0-7.3). We also examine the effect of short-pulse modulation on system performance at the optical receiver by measuring the bit error rate (BER) of received data (1.25 Gb/s). The BER is basically unaffected by the PF (1.0-5.5). This means that the proposed technique has little influence on data reception as regards PF (1.0-5.5).

Anderson localized modes in a disordered glass optical fiber

NASA Astrophysics Data System (ADS)

Karbasi, Salman; Hosseini, Seyedrasoul; Koch, Karl W.; Hawkins, Thomas; Ballato, John; Mafi, Arash

2014-02-01

A beam of light can propagate in a time-invariant transversely disordered waveguide because of transverse Anderson localization. We developed a disordered glass optical ber from a porous artisan glass (satin quartz). The refractive index pro le of the disordered glass optical ber is composed of a non-uniform distribution of air voids which can be approximated as longitudinally invariant. The ll-fraction of air voids is higher at the regions closer to the boundary compared with the central regions. The experimental results show that the beam radius of a localized beam is smaller at the regions closer to the boundary than the one at the central regions. In order to understand the reason behind these observations, the fully vectorial modes of the disordered glass ber are calculated using the actual scanning electron microscope image of the ber tip. The numerical calculations show that the modes at regions closer to the boundary of the ber are more localized compared with the modes at the central regions. Coupling of an input beam to the less-localized modes with large tails at the central regions of the ber results in a large beam radius. In comparison, a beam of light launched at the regions close to the boundary couples to the highly compact modes of the ber and results in a small localized beam radius.

The effect of pyramiding Phytophthora infestans resistance genes RPi-mcd1 and RPi-ber in potato

PubMed Central

Tan, M. Y. Adillah; Hutten, Ronald C. B.; Visser, Richard G. F.

2010-01-01

Despite efforts to control late blight in potatoes by introducing Rpi-genes from wild species into cultivated potato, there are still concerns regarding the durability and level of resistance. Pyramiding Rpi-genes can be a solution to increase both durability and level of resistance. In this study, two resistance genes, RPi-mcd1 and RPi-ber, introgressed from the wild tuber-bearing potato species Solanum microdontum and S. berthaultii were combined in a diploid S. tuberosum population. Individual genotypes from this population were classified after four groups, carrying no Rpi-gene, with only RPi-mcd1, with only RPi-ber, and a group with the pyramided RPi-mcd1 and RPi-ber by means of tightly linked molecular markers. The levels of resistance between the groups were compared in a field experiment in 2007. The group with RPi-mcd1 showed a significant delay to reach 50% infection of the leaf area of 3 days. The group with RPi-ber showed a delay of 3 weeks. The resistance level in the pyramid group suggested an additive effect of RPi-mcd1 with RPi-ber. This suggests that potato breeding can benefit from combining individual Rpi-genes, irrespective of the weak effect of RPi-mcd1 or the strong effect of RPi-ber. PMID:20204320

Richtfunktechnik

NASA Astrophysics Data System (ADS)

Plaßmann, Wilfried

Die Nachrichtenübertragung über Richtfunkstrecken wird neben der Übertragung über Kabel eingesetzt. Gegenüber der Rundfunk- und Fernsehübertragung weist sie den Unterschied auf, dass Sender und Empfänger ortsfest sind. Da es nur einen Empfänger gibt, kann die Übertragung mit einem gerichteten elektromagnetischen Feld erfolgen. Die Besonderheit dieser Technik wird hier dargestellt.

Confirmatory Factor Analysis of the "Preschool Behavioral and Emotional Rating Scale" (PreBERS) with Preschool Children with Disabilities

ERIC Educational Resources Information Center

Cress, Cynthia J.; Synhorst, Lori; Epstein, Michael H.; Allen, Elizabeth

2012-01-01

The "Preschool Behavioral and Emotional Rating Scale" (PreBERS) is a standardized, norm-referenced instrument that assesses emotional and behavioral strengths of preschool children. This study investigated whether the PreBERS four-factor structure (i.e., emotional regulation, school readiness, social confidence, and family involvement)…

Decreased expression level of BER genes in Alzheimer's disease patients is not derivative of their DNA methylation status.

PubMed

Sliwinska, Agnieszka; Sitarek, Przemysław; Toma, Monika; Czarny, Piotr; Synowiec, Ewelina; Krupa, Renata; Wigner, Paulina; Bialek, Katarzyna; Kwiatkowski, Dominik; Korycinska, Anna; Majsterek, Ireneusz; Szemraj, Janusz; Galecki, Piotr; Sliwinski, Tomasz

2017-10-03

Neurodegeneration in Alzheimer's disease can be caused by accumulation of oxidative DNA damage resulting from altered expression of genes involved in the base excision repair system (BER). Promoter methylation can affect the profile of BER genes expression. Decreased expression of BER genes was observed in the brains of AD patients. The aim of our study was to compare the expression and methylation profiles of six genes coding for proteins involved in BER, namely: hOGG1, APE1, MUTYH, NEIL1, PARP1 and XRCC1, in the peripheral blood cells of AD patients and healthy volunteers. The study consisted of 100 persons diagnosed with Alzheimer's disease according to DSM-IV criteria, and 110 healthy volunteers. DNA and total RNA were isolated from venous blood cells. Promoter methylation profiles were obtained by High Resolution Melting (HRM) analysis of bisulfide converted DNA samples. Real-time PCR with TaqMan probes was employed for gene expression analysis. APE1, hOGG1, MUTYH, PARP1 and NEIL1 were significantly (p<0.001) down-regulated in the lymphocytes of AD patients, as compared to healthy volunteers. Expression of XRCC1 didn't differ significantly between both groups. We did not find any differences in the methylation pattern of any of the investigated BER genes. The methylation status of promoters is not associated with downregulation of BER genes. Our results show that downregulation of BER genes detected in peripheral blood samples could reflect the changes occurring in the brain of patients with AD, and may be a useful biomarker of this disease. Copyright © 2017 Elsevier Inc. All rights reserved.

Role of relaxin-3/RXFP3 system in stress-induced binge-like eating in female rats.

PubMed

Calvez, Juliane; de Ávila, Camila; Matte, Louis-Olivier; Guèvremont, Geneviève; Gundlach, Andrew L; Timofeeva, Elena

2016-03-01

Binge eating is frequently stimulated by stress. The neuropeptide relaxin-3 (RLN3) and its native receptor RXFP3 are implicated in stress and appetitive behaviors. We investigated the dynamics of the central RLN3/RXFP3 system in a newly established model of stress-induced binge eating. Female Sprague-Dawley rats were subjected to unpredictable intermittent 1-h access to 10% sucrose. When sucrose intake stabilized, rats were assessed for consistency of higher or lower sucrose intake in response to three unpredictable episodes of foot-shock stress; and assigned as binge-like eating prone (BEP) or binge-like eating resistant (BER). BEP rats displayed elevated consumption of sucrose under non-stressful conditions (30% > BER) and an additional marked increase in sucrose intake (60% > BER) in response to stress. Conversely, sucrose intake in BER rats was unaltered by stress. Chow intake was similar in both phenotypes on 'non-stress' days, but was significantly reduced by stress in BER, but not BEP, rats. After stress, BEP, but not BER, rats displayed a significant increase in RLN3 mRNA levels in the nucleus incertus. In addition, in response to stress, BEP, but not BER, rats had increased RXFP3 mRNA levels in the paraventricular and supraoptic nuclei of the hypothalamus. Intracerebroventricular administration of a selective RXFP3 antagonist, R3(B1-22)R, blocked the stress-induced increase in sucrose intake in BEP rats and had no effect on sucrose intake in BER rats. These results provide important evidence for a role of the central RLN3/RXFP3 system in the regulation of stress-induced binge eating in rats, and have therapeutic implications for eating disorders. Copyright © 2015 Elsevier Ltd. All rights reserved.

Joint Frequency-Domain Equalization and Despreading for Multi-Code DS-CDMA Using Cyclic Delay Transmit Diversity

NASA Astrophysics Data System (ADS)

Yamamoto, Tetsuya; Takeda, Kazuki; Adachi, Fumiyuki

Frequency-domain equalization (FDE) based on the minimum mean square error (MMSE) criterion can provide a better bit error rate (BER) performance than rake combining. To further improve the BER performance, cyclic delay transmit diversity (CDTD) can be used. CDTD simultaneously transmits the same signal from different antennas after adding different cyclic delays to increase the number of equivalent propagation paths. Although a joint use of CDTD and MMSE-FDE for direct sequence code division multiple access (DS-CDMA) achieves larger frequency diversity gain, the BER performance improvement is limited by the residual inter-chip interference (ICI) after FDE. In this paper, we propose joint FDE and despreading for DS-CDMA using CDTD. Equalization and despreading are simultaneously performed in the frequency-domain to suppress the residual ICI after FDE. A theoretical conditional BER analysis is presented for the given channel condition. The BER analysis is confirmed by computer simulation.

Base Excision Repair

PubMed Central

Krokan, Hans E.; Bjørås, Magnar

2013-01-01

Base excision repair (BER) corrects DNA damage from oxidation, deamination and alkylation. Such base lesions cause little distortion to the DNA helix structure. BER is initiated by a DNA glycosylase that recognizes and removes the damaged base, leaving an abasic site that is further processed by short-patch repair or long-patch repair that largely uses different proteins to complete BER. At least 11 distinct mammalian DNA glycosylases are known, each recognizing a few related lesions, frequently with some overlap in specificities. Impressively, the damaged bases are rapidly identified in a vast excess of normal bases, without a supply of energy. BER protects against cancer, aging, and neurodegeneration and takes place both in nuclei and mitochondria. More recently, an important role of uracil-DNA glycosylase UNG2 in adaptive immunity was revealed. Furthermore, other DNA glycosylases may have important roles in epigenetics, thus expanding the repertoire of BER proteins. PMID:23545420

BER Analysis of Coherent Free-Space Optical Communication Systems with a Focal-Plane-Based Wavefront Sensor

NASA Astrophysics Data System (ADS)

Cao, Jingtai; Zhao, Xiaohui; Liu, Wei; Gu, Haijun

2018-03-01

A wavefront sensor is one of most important units for an adaptive optics system. Based on our previous works, in this paper, we discuss the bit-error-rate (BER) performance of coherent free space optical communication systems with a focal-plane-based wavefront sensor. Firstly, the theory of a focal-plane-based wavefront sensor is given. Then the relationship between the BER and the mixing efficiency with a homodyne receiver is discussed on the basis of binary-phase-shift-keying (BPSK) modulation. Finally, the numerical simulation results are shown that the BER will be decreased obviously after aberrations correction with the focal-plane-based wavefront sensor. In addition, the BER will decrease along with increasing number of photons received within a single bit. These analysis results will provide a reference for the design of the coherent Free space optical communication (FSOC) system.

Fiber-Bragg-Grating-Based Optical Code-Division Multiple Access Passive Optical Network Using Dual-Baseband Modulation Scheme

NASA Astrophysics Data System (ADS)

Lin, Wen-Piao; Wu, He-Long

2005-08-01

We propose a fiber-Bragg-grating (FBG)-based optical code-division multiple access passive optical network (OCDMA-PON) using a dual-baseband modulation scheme. A mathematical model is developed to study the performance of this scheme. According to the analyzed results, this scheme can allow a tolerance of the spectral power distortion (SPD) ratio of 25% with a bit error rate (BER) of 10-9 when the modified pseudorandom noise (PN) code length is 16. Moreover, we set up a simulated system to evaluate the baseband and radio frequency (RF) band transmission characteristics. The simulation results demonstrate that our proposed OCDMA-PON can provide a cost-effective and scalable fiber-to-the-home solution.

Performance of Complex Spreading MIMO Systems With Interference

DTIC Science & Technology

2011-06-01

14 III. PERFORMANCE ANALYSIS OF DS -PSK MISO ...............................................15 A. SYSTEM DESCRIPTION...15 Figure 9. BER of DS PSK system for broadband jamming and diversity L=1. ..............20 Figure 10. BER of DS PSK system for...broadband jamming and diversity L=2. .............21 Figure 11. BER of DS PSK system for broadband jamming and diversity L=3. ..............22 Figure 12

Conditions for the optical wireless links bit error ratio determination

NASA Astrophysics Data System (ADS)

Kvíčala, Radek

2017-11-01

To determine the quality of the Optical Wireless Links (OWL), there is necessary to establish the availability and the probability of interruption. This quality can be defined by the optical beam bit error rate (BER). Bit error rate BER presents the percentage of successfully transmitted bits. In practice, BER runs into the problem with the integration time (measuring time) determination. For measuring and recording of BER at OWL the bit error ratio tester (BERT) has been developed. The 1 second integration time for the 64 kbps radio links is mentioned in the accessible literature. However, it is impossible to use this integration time for singularity of coherent beam propagation.

A New Closed Form Approximation for BER for Optical Wireless Systems in Weak Atmospheric Turbulence

NASA Astrophysics Data System (ADS)

Kaushik, Rahul; Khandelwal, Vineet; Jain, R. C.

2018-04-01

Weak atmospheric turbulence condition in an optical wireless communication (OWC) is captured by log-normal distribution. The analytical evaluation of average bit error rate (BER) of an OWC system under weak turbulence is intractable as it involves the statistical averaging of Gaussian Q-function over log-normal distribution. In this paper, a simple closed form approximation for BER of OWC system under weak turbulence is given. Computation of BER for various modulation schemes is carried out using proposed expression. The results obtained using proposed expression compare favorably with those obtained using Gauss-Hermite quadrature approximation and Monte Carlo Simulations.

The magnetic field of gastrointestinal smooth muscle activity

NASA Astrophysics Data System (ADS)

Bradshaw, Alan; Ladipo, Jk; Richards, William; Wikswo, John

1997-11-01

The gastrointestinal (GI) tract controls the absorption and transport of ingested materials. Its function is determined largely by the electrical activity of the smooth muscle that lines the GI tract. GI electrical activity consists of an omnipresent slowly oscillating wave known as the basic electrical rhythm (BER) that modulates a higher-frequency spiking activity associated with muscle contraction. The BER has been shown to be a reliable indicator of intestinal viability, and thus, recording of smooth muscle activity may have clinical value. The BER is difficult to measure with cutaneous electrodes because layers of low-conductivity fat between the GI tract and the abdominal surface attenuate the potential. On the other hand, the magnetic field associated with GI electrical activity is mostly unaffected by intervening fat layers. We recorded the magnetic fields from GI activity in 12 volunteers using a multichannel Superconducting QUantum Interference Device (SQUID) magnetometer. Characteristics typical of gastric and intestinal BER were apparent in the data. Channels near the epigastrium recorded gastric BER, and channels in intestinal areas recorded small bowel BER. These results suggest that a single multichannel SQUID magnetometer is able to measure gastrointestinal electrical activity from multiple locations around the abdomen simultaneously.

Overexpression of DNA ligase III in mitochondria protects cells against oxidative stress and improves mitochondrial DNA base excision repair.

PubMed

Akbari, Mansour; Keijzers, Guido; Maynard, Scott; Scheibye-Knudsen, Morten; Desler, Claus; Hickson, Ian D; Bohr, Vilhelm A

2014-04-01

Base excision repair (BER) is the most prominent DNA repair pathway in human mitochondria. BER also results in a temporary generation of AP-sites, single-strand breaks and nucleotide gaps. Thus, incomplete BER can result in the generation of DNA repair intermediates that can disrupt mitochondrial DNA replication and transcription and generate mutations. We carried out BER analysis in highly purified mitochondrial extracts from human cell lines U2OS and HeLa, and mouse brain using a circular DNA substrate containing a lesion at a specific position. We found that DNA ligation is significantly slower than the preceding mitochondrial BER steps. Overexpression of DNA ligase III in mitochondria improved the rate of overall BER, increased cell survival after menadione induced oxidative stress and reduced autophagy following the inhibition of the mitochondrial electron transport chain complex I by rotenone. Our results suggest that the amount of DNA ligase III in mitochondria may be critical for cell survival following prolonged oxidative stress, and demonstrate a functional link between mitochondrial DNA damage and repair, cell survival upon oxidative stress, and removal of dysfunctional mitochondria by autophagy. Copyright © 2014. Published by Elsevier B.V.

Aerial Observation Needs Workshop, May 13-14, 2015

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nasiri, Shaima; Serbin, Shawn; Lesmes, David

2015-10-01

The mission of the Climate and Environmental Sciences Division (CESD) of the Office of Biological and Environmental Research (BER) within the U.S. Department of Energy's (DOE) Office of Science is "to advance a robust, predictive understanding of Earth's climate and environmental systems and to inform the development of sustainable solutions to the nation's energy and environmental challenges." Accomplishing this mission requires aerial observations of the atmospheric and terrestrial components of the climate system. CESD is assessing its current and future aerial observation needs to develop a strategy and roadmap of capability requirements for the next decade. To facilitate this process,more » a workshop was convened that consisted of invited experts in the atmospheric and terrestrial sciences, airborne observations, and modeling. This workshop report summarizes the community input prior to and during the workshop on research challenges and opportunities, as well as specific science questions and observational needs that require aerial observations to address.« less

Suppression of optical beat interference-noise in orthogonal frequency division multiple access-passive optical network link using self-homodyne balanced detection

NASA Astrophysics Data System (ADS)

Won, Yong-Yuk; Jung, Sang-Min; Han, Sang-Kook

2014-08-01

A new technique, which reduces optical beat interference (OBI) noise in orthogonal frequency division multiple access-passive optical network (OFDMA-PON) links, is proposed. A self-homodyne balanced detection, which uses a single laser for the optical line terminal (OLT) as well as for the optical network unit (ONU), reduces OBI noise and also improves the signal to noise ratio (SNR) of the discrete multi-tone (DMT) signal. The proposed scheme is verified by transmitting quadrature phase shift keying (QPSK)-modulated DMT signal over a 20-km single mode fiber. The optical signal to noise ratio (OSNR), that is required for BER of 10-5, is reduced by 2 dB in the balanced detection compared with a single channel due to the cancellation of OBI noise in conjunction with the local laser.

Protocol Support for a New Satellite-Based Airspace Communication Network

NASA Technical Reports Server (NTRS)

Shang, Yadong; Hadjitheodosiou, Michael; Baras, John

2004-01-01

We recommend suitable transport protocols for an aeronautical network supporting Internet and data services via satellite. We study the characteristics of an aeronautical satellite hybrid network and focus on the problems that cause dramatically degraded performance of the Transport Protocol. We discuss various extensions to standard TCP that alleviate some of these performance problems. Through simulation, we identify those TCP implementations that can be expected to perform well. Based on the observation that it is difficult for an end-to-end solution to solve these problems effectively, we propose a new TCP-splitting protocol, termed Aeronautical Transport Control Protocol (AeroTCP). The main idea of this protocol is to use a fixed window for flow control and one duplicated acknowledgement (ACK) for fast recovery. Our simulation results show that AeroTCP can maintain higher utilization for the satellite link than end-to-end TCP, especially in high BER environment.

Physikgeschichte Das Rätsel bleibt

NASA Astrophysics Data System (ADS)

Hoffmann, Dieter

2002-05-01

Die jüngst erfolgte Veröffentlichung von Dokumenten aus dem Privatarchiv der Familie Bohr [1] über den geheimnisumwitterten Besuch von Werner Heisenberg in Kopenhagen im September 1941 hat in den deutschen Medien große Resonanz gefunden [2]. Tatsächlich verraten uns die jetzt veröffentlichten Dokumente sehr viel mehr über die Zeit ihres Entstehens als über den Besuch selbst.

Draft Genome Sequence of Desulfovibrio BerOc1, a Mercury-Methylating Strain

PubMed Central

Gassie, Claire; Bouchez, Oliver; Klopp, Christophe; Guyoneaud, Rémy

2017-01-01

ABSTRACT Desulfovibrio BerOc1 is a sulfate-reducing bacterium isolated from the Berre lagoon (French Mediterranean coast). BerOc1 is able to methylate and demethylate mercury. The genome size is 4,081,579 bp assembled into five contigs. We identified the hgcA and hgcB genes involved in mercury methylation, but not those responsible for mercury demethylation. PMID:28104657

Experimental demonstration of bidirectional up to 40 Gbit/s QPSK coherent free-space optical communication link over ∼1 km

NASA Astrophysics Data System (ADS)

Feng, Xianglian; Wu, Zhihang; Wang, Tianshu; Zhang, Peng; Li, Xiaoyan; Jiang, Huilin; Su, Yuwei; He, Hongwei; Wang, Xiaoyan; Gao, Shiming

2018-03-01

Advanced multi-level modulation formats have shown their great potential in high-speed and high-spectral-efficiency optical communications. Using quadrature phase-shift keying (QPSK) modulation format for free-space optical (FSO) communication, a bidirectional high-speed FSO transmission link with the bit rates of up to 40 Gbit/s over ∼1 km, between two buildings in the campus of Changchun University of Science and Technology, Changchun, China, is experimentally demonstrated cooperating by capture and tracking systems. The eye-diagrams and constellation diagrams of the transmitted QPSK signals are clearly observed. By comparing the bit error rate (BER) curves before and after transmission, one can find that the receiving powers are both less than -16.5 dBm for the forward and backward transmissions of the bidirectional 20, 30, and 40 Gbit/s FSO links, and their power penalties due to the phase fluctuation of the atmospheric channel are both less than 2.6 dB, at the BER of 3.8 ×10-3.

Research in progress: FY 1992. Summaries of projects

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-08-01

The Biological and Environmental Research (BER) Program of OHER has two main missions: (1) to develop the knowledge base necessary to identify, understand, and anticipate the long-term health and environmental consequences of energy use and development and (2) to utilize the Department`s unique scientific and technological capabilities to solve major scientific problems in medicine, biology, and the environment. These missions reflect a commitment to develop the beneficial uses of advanced energy technologies while at the same time assuring that any potentially adverse health and environmental impacts of the Nation`s energy policies are fully identified and understood. The BER Program includesmore » research in atmospheric, marine, and terrestrial processes, including the linkage between the use in greenhouse gases, carbon dioxide, and regional and global climate change; in molecular and subcellular mechanisms underlying human somatic and genetic processes and their responses to energy-related environmental toxicants; in nuclear medicine, structural biology, the human genome, measurement sciences and instrumentation, and other areas that require the unique capabilities of the Department`s laboratory system. The principal areas of research are Health Research and Environmental Research.« less

Adaptively combined FIR and functional link artificial neural network equalizer for nonlinear communication channel.

PubMed

Zhao, Haiquan; Zhang, Jiashu

2009-04-01

This paper proposes a novel computational efficient adaptive nonlinear equalizer based on combination of finite impulse response (FIR) filter and functional link artificial neural network (CFFLANN) to compensate linear and nonlinear distortions in nonlinear communication channel. This convex nonlinear combination results in improving the speed while retaining the lower steady-state error. In addition, since the CFFLANN needs not the hidden layers, which exist in conventional neural-network-based equalizers, it exhibits a simpler structure than the traditional neural networks (NNs) and can require less computational burden during the training mode. Moreover, appropriate adaptation algorithm for the proposed equalizer is derived by the modified least mean square (MLMS). Results obtained from the simulations clearly show that the proposed equalizer using the MLMS algorithm can availably eliminate various intensity linear and nonlinear distortions, and be provided with better anti-jamming performance. Furthermore, comparisons of the mean squared error (MSE), the bit error rate (BER), and the effect of eigenvalue ratio (EVR) of input correlation matrix are presented.

Optical signal suppression by a cascaded SOA/RSOA for wavelength reusing reflective PON upstream transmission.

PubMed

Jung, Sang Min; Mun, Kyoung Hak; Kang, Soo Min; Han, Sang Kook

2017-09-18

An optical signal suppression technique based on a cascaded SOA and RSOA is proposed for the reflective passive optical networks (PONs) with wavelength division multiplexing (WDM). By suppressing the downstream signal of the optical carrier, the proposed reflective PON effectively reuses the downstream optical carrier for upstream signal transmission. As an experimental demonstration, we show that the proposed optical signal suppression technique is effective in terms of the signal bandwidth and bit-error-rate (BER) performance of the remodulated upstream transmission.

Developing an in silico model of the modulation of base excision repair using methoxyamine for more targeted cancer therapeutics.

PubMed

Gurkan-Cavusoglu, Evren; Avadhani, Sriya; Liu, Lili; Kinsella, Timothy J; Loparo, Kenneth A

2013-04-01

Base excision repair (BER) is a major DNA repair pathway involved in the processing of exogenous non-bulky base damages from certain classes of cancer chemotherapy drugs as well as ionising radiation (IR). Methoxyamine (MX) is a small molecule chemical inhibitor of BER that is shown to enhance chemotherapy and/or IR cytotoxicity in human cancers. In this study, the authors have analysed the inhibitory effect of MX on the BER pathway kinetics using a computational model of the repair pathway. The inhibitory effect of MX depends on the BER efficiency. The authors have generated variable efficiency groups using different sets of protein concentrations generated by Latin hypercube sampling, and they have clustered simulation results into high, medium and low efficiency repair groups. From analysis of the inhibitory effect of MX on each of the three groups, it is found that the inhibition is most effective for high efficiency BER, and least effective for low efficiency repair.

Effect of Pointing Error on the BER Performance of an Optical CDMA FSO Link with SIK Receiver

NASA Astrophysics Data System (ADS)

Nazrul Islam, A. K. M.; Majumder, S. P.

2017-12-01

An analytical approach is presented for an optical code division multiple access (OCDMA) system over free space optical (FSO) channel considering the effect of pointing error between the transmitter and the receiver. Analysis is carried out with an optical sequence inverse keying (SIK) correlator receiver with intensity modulation and direct detection (IM/DD) to find the bit error rate (BER) with pointing error. The results are evaluated numerically in terms of signal-to-noise plus multi-access interference (MAI) ratio, BER and power penalty due to pointing error. It is noticed that the OCDMA FSO system is highly affected by pointing error with significant power penalty at a BER of 10-6 and 10-9. For example, penalty at BER 10-9 is found to be 9 dB corresponding to normalized pointing error of 1.4 for 16 users with processing gain of 256 and is reduced to 6.9 dB when the processing gain is increased to 1,024.

Bioenergetic metabolites regulate base excision repair dependent cell death in response to DNA damage

PubMed Central

Tang, Jiang-bo; Goellner, Eva M.; Wang, Xiao-hong; Trivedi, Ram N.; Croix, Claudette M. St; Jelezcova, Elena; Svilar, David; Brown, Ashley R.; Sobol, Robert W.

2009-01-01

Base excision repair (BER) protein expression is important for resistance to DNA damage-induced cytotoxicity. Conversely, BER imbalance (Polß deficiency or repair inhibition) enhances cytotoxicity of radiation and chemotherapeutic DNA-damaging agents. Whereas inhibition of critical steps in the BER pathway result in the accumulation of cytotoxic DNA double-strand breaks, we report that DNA damage-induced cytotoxicity due to deficiency in the BER protein Polß triggers cell death dependent on PARP activation yet independent of poly(ADP-ribose) (PAR)-mediated AIF nuclear translocation or PARG, suggesting that cytotoxicity is not from PAR or PAR-catabolite signaling. Cell death is rescued by the NAD+ metabolite NMN and is synergistic with inhibition of NAD+ biosynthesis, demonstrating that DNA damage-induced cytotoxicity mediated via BER inhibition is primarily dependent on cellular metabolite bioavailability. We offer a mechanistic justification for the elevated alkylation-induced cytotoxicity of Polß deficient cells, suggesting a linkage between DNA repair, cell survival and cellular bioenergetics. PMID:20068071

Analysis of physical layer performance of hybrid optical-wireless access network

NASA Astrophysics Data System (ADS)

Shaddad, R. Q.; Mohammad, A. B.; Al-hetar, A. M.

2011-09-01

The hybrid optical-wireless access network (HOWAN) is a favorable architecture for next generation access network. It is an optimal combination of an optical backhaul and a wireless front-end for an efficient access network. In this paper, the HOWAN architecture is designed based on a wavelengths division multiplexing/time division multiplexing passive optical network (WDM/TDM PON) at the optical backhaul and a wireless fidelity (WiFi) technology at the wireless front-end. The HOWAN is proposed that can provide blanket coverage of broadband and flexible connection for end-users. Most of the existing works, based on performance evaluation are concerned on network layer aspects. This paper reports physical layer performance in terms of the bit error rate (BER), eye diagram, and signal-to-noise ratio (SNR) of the communication system. It accommodates 8 wavelength channels with 32 optical network unit/wireless access points (ONU/APs). It is demonstrated that downstream and upstream of 2 Gb/s can be achieved by optical backhaul for each wavelength channel along optical fiber length of 20 km and a data rate of 54 Mb/s per ONU/AP along a 50 m outdoor wireless link.

Contribution of DNA unwrapping from histone octamers to the repair of oxidatively damaged DNA in nucleosomes

PubMed Central

Maher, Robyn L.; Prasad, Amalthiya; Rizvanova, Olga; Wallace, Susan S.; Pederson, David S.

2013-01-01

Reactive oxygen species generate ~20,000 oxidative lesions in the DNA of every cell, every day. Most of these lesions are located within nucleosomes, which package DNA in chromatin and impede base excision repair (BER). We demonstrated previously that periodic, spontaneous partial unwrapping of DNA from the underlying histone octamer enables BER enzymes to bind to oxidative lesions that would otherwise be sterically inaccessible. In the present study, we asked if these periodic DNA unwrapping events are frequent enough to account for the estimated rates of BER in vivo. We measured rates of excision of oxidative lesions from sites in nucleosomes that are accessible only during unwrapping episodes. Using reaction conditions appropriate for presteady-state kinetic analyses, we derived lesion exposure rates for both 601 and 5S rDNA-based nucleosomes. Although DNA unwrapping-mediated exposure of a lesion ~16 NT from the nucleosome edge occurred ~7–8 times per minute, exposure rates fell dramatically for lesions located 10 or more NT further in from the nucleosome edge. The rates likely are too low to account for observed rates of BER in cells. Thus, chromatin remodeling, either BER-specific or that associated with transcription, replication, or other DNA repair processes, probably contributes to efficient BER in vivo. PMID:24051050

Glutathione homeostasis as an important and novel factor controlling blossom-end rot development in calcium-deficient tomato fruits.

PubMed

Mestre, Teresa C; Garcia-Sanchez, Francisco; Rubio, Francisco; Martinez, Vicente; Rivero, Rosa M

2012-11-15

Based on previous results in which oxidative metabolism was suggested as a possible inducer of blossom-end rot (BER), the main questions addressed here were whether calcium deficiency is the main factor that induces BER or whether this physiological disorder a general stress-related phenomenon? Tomato plants were grown under optimal or deficient calcium concentrations. Only the application of 0.1mM calcium resulted in BER induction, although only half of the fruits grown under this treatment had this disorder. Having fruits showing or not showing BER in the same plant and treatment provided us with a powerful tool that we used to investigate whether calcium deficiency operates alongside another mechanism in the induction of BER. Whether or not this other mechanism was the one controlling BER incidence was also investigated. We performed a complete study of the oxidative metabolism in the pericarp of healthy fruits and in the healthy portion of BER-affected fruits. Calcium deficiency led to an induction of NADPH oxidase, superoxide dismutase, dehydro- and monodehydroascorbate reductase, and to an inhibition of catalase, ascorbate peroxidase and glutathione reductase, with a concomitant accumulation of hydrogen peroxide and an increase in lipid peroxidation. While the ascorbate redox state was not affected by calcium deficiency, the glutathione redox state was markedly reduced. We conclude that calcium deficiency fundamentally affected the activity of the ascorbate-glutathione enzymes, with special importance to the inhibition of GR, which lead to a reduction of the glutathione redox state. This could cause the breakdown of cellular homeostasis, the inhibition of other enzymes responsible for H(2)O(2) detoxification, and ultimately an increase of lipid peroxidation. Therefore, BER is defined here as the visual symptom of a massive lipid peroxidation event caused by the breakdown of cellular glutathione homeostasis. Copyright © 2012 Elsevier GmbH. All rights reserved.

Mitochondrial base excision repair in mouse synaptosomes during normal aging and in a model of Alzheimer’s disease

PubMed Central

Gredilla, Ricardo; Weissman, Lior; Yang, Jenq-Lin; Bohr, Vilhelm A.; Stevnsner, Tinna

2010-01-01

Brain aging is associated with synaptic decline and cognitive impairment. Increased levels of oxidative DNA base damage and accumulation of mitochondrial DNA (mtDNA) mutations or deletions lead to mitochondrial dysfunction, playing an important role in the aging process and the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease (AD). In mitochondria, base excision repair (BER) is the main DNA repair pathway for base modifications such as deamination and oxidation, and constitutes an important mechanism to avoid accumulation of mtDNA mutations. Synaptic function is highly dependent on mitochondria, and in the current study we have investigated BER in synaptosomes of mouse brain during normal aging and in an AD model. Synaptosomes are isolated synapses in membranous structures produced by subcellular fractionation of brain tissue. They include the whole presynaptic terminal as well as portions of the postsynaptic terminal. Synaptosomes contain the molecular machinery necessary for uptake, storage, and release of neurotransmitters, including synaptic vesicles and mitochondria. BER activities were measured in synaptosomal fractions from young and old mice and from pre-symptomatic and symptomatic AD mice harboring mutated APP, Tau and PS1 (3xTgAD). During normal aging, a reduction in the BER capacity was observed in the synaptosomal fraction, which was associated with a decrease in the level of BER proteins. However, we did not observe changes between the synaptosomal BER activities of pre-symptomatic and symptomatic AD mice. Our findings suggest that the age-related reduction in BER capacity in the synaptosomal fraction might contribute to mitochondrial and synaptic dysfunction during aging. The development of AD-like pathology in the 3xTgAD mouse model was, however, not associated with deficiencies of the BER mechanisms in the synaptosomal fraction when the whole brain was analyzed. PMID:20708822

Solid dispersion of berberine-phospholipid complex/TPGS 1000/SiO₂: preparation, characterization and in vivo studies.

PubMed

Zhang, Zhenhai; Chen, Yan; Deng, Jin; Jia, Xiaobin; Zhou, Jianping; Lv, Huixia

2014-04-25

Berberine (Ber), an isoquinoline alkaloid, arouses wide interests in many researchers in recent years because of its numerous new pharmacological actions. However Ber's low oral bioavailability restricts its wide application. In this study, a solid dispersion (BPTS-SD) composed of berberine-phospholipid complex (BPC), D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS 1000) and SiO₂ was prepared by simple solvent evaporation technique. BPC was employed to improve the liposolubility of Ber, and SiO₂ was used to improve the flowability of BPTS-SD, while TPGS 1000 played a dual role: firstly, as a solid dispersion carrier to improve the dissolution rate of BPC and secondly, as a P-glycoprotein (P-gp) inhibitor to enhance the intestinal absorption of Ber. FTIR, DSC and SEM analysis proved the formation of BPC and BPTS-SD. Po/w of BPC successfully increased from 0.25 to 8.75. In vitro dissolution study showed that the cumulative dissolution percentages of BPTS-SDs were nearly 2.67-4.78-folds of BPC. Single-pass intestinal perfusion studies showed that the absorption of Ber in BPC was increased nearly 1.4-2.0-folds compared to that of Ber which was mainly due to the improved liposolubility, and further increased by BPTS-SD around 0.1-1.3-folds compared to that of BPC through the P-gp inhibition of TPGS 1000. Significant improvements in Cmax and AUC₀→t of BPC and BPTS-SD were obtained in pharmacokinetic study (the highest improvement in oral relative bioavailability of BPTS-SD-1 was 322.66% of Ber). All these results indicated that BPTS-SD can be a promising drug delivery system to improve their oral bioavailability for the Ber's analogues. In particular this solid dispersion can be prepared just by a simple method and has a strong feasibility for industrialization. Crown Copyright © 2014. Published by Elsevier B.V. All rights reserved.

ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair.

PubMed

Tsuda, Masataka; Cho, Kosai; Ooka, Masato; Shimizu, Naoto; Watanabe, Reiko; Yasui, Akira; Nakazawa, Yuka; Ogi, Tomoo; Harada, Hiroshi; Agama, Keli; Nakamura, Jun; Asada, Ryuta; Fujiike, Haruna; Sakuma, Tetsushi; Yamamoto, Takashi; Murai, Junko; Hiraoka, Masahiro; Koike, Kaoru; Pommier, Yves; Takeda, Shunichi; Hirota, Kouji

2017-01-01

ALC1/CHD1L is a member of the SNF2 superfamily of ATPases carrying a macrodomain that binds poly(ADP-ribose). Poly(ADP-ribose) polymerase (PARP) 1 and 2 synthesize poly(ADP-ribose) at DNA-strand cleavage sites, promoting base excision repair (BER). Although depletion of ALC1 causes increased sensitivity to various DNA-damaging agents (H2O2, UV, and phleomycin), the role played by ALC1 in BER has not yet been established. To explore this role, as well as the role of ALC1's ATPase activity in BER, we disrupted the ALC1 gene and inserted the ATPase-dead (E165Q) mutation into the ALC1 gene in chicken DT40 cells, which do not express PARP2. The resulting ALC1-/- and ALC1-/E165Q cells displayed an indistinguishable hypersensitivity to methylmethane sulfonate (MMS), an alkylating agent, and to H2O2, indicating that ATPase plays an essential role in the DNA-damage response. PARP1-/- and ALC1-/-/PARP1-/- cells exhibited a very similar sensitivity to MMS, suggesting that ALC1 and PARP1 collaborate in BER. Following pulse-exposure to H2O2, PARP1-/- and ALC1-/-/PARP1-/- cells showed similarly delayed kinetics in the repair of single-strand breaks, which arise as BER intermediates. To ascertain ALC1's role in BER in mammalian cells, we disrupted the ALC1 gene in human TK6 cells. Following exposure to MMS and to H2O2, the ALC1-/- TK6 cell line showed a delay in single-strand-break repair. We therefore conclude that ALC1 plays a role in BER. Following exposure to H2O2, ALC1-/- cells showed compromised chromatin relaxation. We thus propose that ALC1 is a unique BER factor that functions in a chromatin context, most likely as a chromatin-remodeling enzyme.

A continuous flow MFC-CW coupled with a biofilm electrode reactor to simultaneously attenuate sulfamethoxazole and its corresponding resistance genes.

PubMed

Li, Hua; Song, Hai-Liang; Yang, Xiao-Li; Zhang, Shuai; Yang, Yu-Li; Zhang, Li-Min; Xu, Han; Wang, Ya-Wen

2018-05-08

A continuous flow microbial fuel cell constructed wetland (MFC-CW) coupled with a biofilm electrode reactor (BER) system was constructed to remove sulfamethoxazole (SMX). The BER unit powered by the stacked MFC-CWs was used as a pretreatment unit, and effluent flowed into the MFC-CW for further degradation. The experimental results indicated that the removal rate of 2 or 4 mg/L SMX in a BER unit was nearly 90%, and the total removal rate in the coupled system was over 99%. As the hydraulic retention time (HRT) was reduced from 16 h to 4 h, the SMX removal rate in the BER decreased from 75% to 48%. However, the total removal rate in the coupled system was still over 97%. The maximum SMX removal rate in the MFC-CW, which accounted for 42%-55% of the total removal, was obtained in the anode layer. In addition, the relative abundances of sul genes detected in the systems were in the order of sulI > sulII > sulIII, and significant positive correlations of sul gene copy numbers versus SMX concentration and 16S rRNA gene copy numbers were observed. Furthermore, significant negative correlations were identified between sul genes, 16S rRNA gene copy numbers, and HRT. The abundances of the sul genes in the effluent of the MFC-CW were lower than the abundances observed in the BER effluent. High-throughput sequencing revealed that the microbial community diversity of the BER was affected by running time, power supply forms and HRT. Bio-electricity from the MFC-CW may reduce microbial community diversity and contribute to reduction of the antibiotic resistance gene (ARG) abundance in the BER. Taken together, the BER-MFC-CW coupled system is a potential tool to treat wastewater containing SMX and attenuate corresponding ARG abundance. Copyright © 2018 Elsevier B.V. All rights reserved.

Draft Genome Sequence of Desulfovibrio BerOc1, a Mercury-Methylating Strain.

PubMed

Goñi Urriza, Marisol; Gassie, Claire; Bouchez, Oliver; Klopp, Christophe; Guyoneaud, Rémy

2017-01-19

Desulfovibrio BerOc1 is a sulfate-reducing bacterium isolated from the Berre lagoon (French Mediterranean coast). BerOc1 is able to methylate and demethylate mercury. The genome size is 4,081,579 bp assembled into five contigs. We identified the hgcA and hgcB genes involved in mercury methylation, but not those responsible for mercury demethylation. Copyright © 2017 Goñi Urriza et al.

Ber-H2 (CD30) immunohistochemical staining in malignant melanoma.

PubMed

Polski, J M; Janney, C G

1999-09-01

Malignant melanoma can be included in the differential diagnosis of Hodgkin's disease, anaplastic large cell lymphoma, or embryonal carcinoma These malignancies express CD30, a marker of diagnostic value. A retrospective immunohistochemical study was undertaken to determine the frequency of immunoreactivity of Ber-H2 (anti-CD30 monoclonal antibody) in malignant melanoma Archival paraffin-embedded tissue from 24 primary and metastatic lesions was used. No Ber-H2 labeling was observed in the majority of the studied cases. Variable weak cytoplasmic staining was present in only one case. The findings are compared with the previous reports claiming frequent CD30 expression in malignant melanoma. We discuss issues pertaining to the interpretation of the Ber-H2 IHC staining in formalin-fixed, paraffin-embedded tissue.

Mechanism of Adenomatous Polyposis Coli (APC)-mediated Blockage of Longpatch Base Excision Repair†

PubMed Central

Jaiswal, Aruna S.; Balusu, Ramesh; Armas, Melissa L.; Kundu, Chanakya N.; Narayan, Satya

2008-01-01

Recently, we found an interaction between adenomatous polyposis coli (APC) and DNA polymerase β (pol-β) and showed that APC blocks strand-displacement synthesis of long-patch base excision repair (LP-BER) however, the mechanism is not clear. Using an in vivo LP-BER assay system, we now show that the LP-BER is higher in APC−/− cells than in APC+/+ cells. In addition to pol-β, the pull-down experiments showed that the full-length APC also interacted with flap endonuclease 1 (Fen-1). To further characterize the interaction of APC with pol-β and Fen-1, we performed a domain-mapping of APC and found that both pol-β and Fen-1 interact with a 138-amino acids peptide from the APC at the DRI-domain. Our functional assays showed that APC blocks pol-β-mediated 1-nucleotide (1-nt) as well as strand-displacement synthesis of reduced abasic, nicked-, or 1-nt gapped-DNA substrates. Our further studies demonstrated that APC blocks 5′-flap endonuclease as well as 5′-3′ exonuclease activity of Fen-1 resulting in the blockage of LP-BER. From these results we concluded that APC can have three different effects in the LP-BER pathway. First, APC can block pol-β-mediated 1-nt incorporation and strand-displacement synthesis. Second, APC can block LP-BER by blocking coordinated formation and removal of the strand-displaced flap. Third, APC can block LP-BER by blocking “Hit and Run” synthesis. These studies will have important implications of APC in DNA damage-induced carcinogenesis and chemoprevention. PMID:17176113

JPRS Report, East Europe

DTIC Science & Technology

1988-02-24

after that, it was Defense Minister Sokolov and 2 weeks later Lev Saikov, a mem - ber of the Politburo and a central committee secretary, visited...POL1TICHESKOYE OBRAZOVAN1YE in Russian No 12 Dec 87 signed to press 16 Nov 87 pp 114-120 [Article by MSZMP Central Committee Politburo Mem - ber and Central...work" which should above all find its expres- sion in: —the growth of political requirements on party mem - bers; —consolidation of a creative

Free-space optical communication link performance enhancement via modified receiver geometric characteristics

NASA Astrophysics Data System (ADS)

Prasad, Narasimha S.; Kratovil, Patrick T.; Tucker, Sara C.; Vallestero, Neil J.; Khusid, Mark

2004-01-01

A free-space, line-of-sight, ground-based optical link at 1.5 microns is attractive for tactical communications because it would provide eye-safety, covertness and jam-proof operation. However, the effects of atmospheric turbulence have to be appropriately mitigated for achieving acceptable bit-error-rate (BER) for reliable dissemination of information. Models to predict achievable BER at 1.5 microns for several beam propagation schemes that include beam scanning have been developed for various turbulence conditions. In this paper, we report performance characterization of free-space, high-data (>1Gb/s) rate beam propagation parameters at 1.5 microns for achieving BER reduction under the presence of turbulence. For standard free-space optical links, the mean SNR limits the achievable BER to lesser than 10-6 for Cn2 (structure constant of refractive index fluctuations) around 10-12 m-2/3. To validate these models, simultaneous measurements of structure constant of refractive index fluctuations, Cn2, and coherence diameter over tactical ranges have been carried out and analyzed. The effect of input beam conditioning to reduce BER levels have been explored. Furthermore, single and multiple transmit beams in conjunction with single and multiple detector arrangements have been examined. Based on these measurements, it is shown that the advantages of input beam conditioning coupled with modified receiver geometric characteristics would provide a path for BER reduction and hence, appreciable enhancements in data link reliability.

An Architectural Concept for Intrusion Tolerance in Air Traffic Networks

NASA Technical Reports Server (NTRS)

Maddalon, Jeffrey M.; Miner, Paul S.

2003-01-01

The goal of an intrusion tolerant network is to continue to provide predictable and reliable communication in the presence of a limited num ber of compromised network components. The behavior of a compromised network component ranges from a node that no longer responds to a nod e that is under the control of a malicious entity that is actively tr ying to cause other nodes to fail. Most current data communication ne tworks do not include support for tolerating unconstrained misbehavio r of components in the network. However, the fault tolerance communit y has developed protocols that provide both predictable and reliable communication in the presence of the worst possible behavior of a limited number of nodes in the system. One may view a malicious entity in a communication network as a node that has failed and is behaving in an arbitrary manner. NASA/Langley Research Center has developed one such fault-tolerant computing platform called SPIDER (Scalable Proces sor-Independent Design for Electromagnetic Resilience). The protocols and interconnection mechanisms of SPIDER may be adapted to large-sca le, distributed communication networks such as would be required for future Air Traffic Management systems. The predictability and reliabi lity guarantees provided by the SPIDER protocols have been formally v erified. This analysis can be readily adapted to similar network stru ctures.

Average BER of subcarrier intensity modulated free space optical systems over the exponentiated Weibull fading channels.

PubMed

Wang, Ping; Zhang, Lu; Guo, Lixin; Huang, Feng; Shang, Tao; Wang, Ranran; Yang, Yintang

2014-08-25

The average bit error rate (BER) for binary phase-shift keying (BPSK) modulation in free-space optical (FSO) links over turbulence atmosphere modeled by the exponentiated Weibull (EW) distribution is investigated in detail. The effects of aperture averaging on the average BERs for BPSK modulation under weak-to-strong turbulence conditions are studied. The average BERs of EW distribution are compared with Lognormal (LN) and Gamma-Gamma (GG) distributions in weak and strong turbulence atmosphere, respectively. The outage probability is also obtained for different turbulence strengths and receiver aperture sizes. The analytical results deduced by the generalized Gauss-Laguerre quadrature rule are verified by the Monte Carlo simulation. This work is helpful for the design of receivers for FSO communication systems.

Characterization of Impulse Radio Intrabody Communication System for Wireless Body Area Networks.

PubMed

Cai, Zibo; Seyedi, MirHojjat; Zhang, Weiwei; Rivet, Francois; Lai, Daniel T H

2017-01-01

Intrabody communication (IBC) is a promising data communication technique for body area networks. This short-distance communication approach uses human body tissue as the medium of signal propagation. IBC is defined as one of the physical layers for the new IEEE 802.15.6 or wireless body area network (WBAN) standard, which can provide a suitable data rate for real-time physiological data communication while consuming lower power compared to that of radio-frequency protocols such as Bluetooth. In this paper, impulse radio (IR) IBC (IR-IBC) is examined using a field-programmable gate array (FPGA) implementation of an IBC system. A carrier-free pulse position modulation (PPM) scheme is implemented using an IBC transmitter in an FPGA board. PPM is a modulation technique that uses time-based pulse characteristics to encode data based on IR concepts. The transmission performance of the scheme was evaluated through signal propagation measurements of the human arm using 4- and 8-PPM transmitters, respectively. 4 or 8 is the number of symbols during modulations. It was found that the received signal-to-noise ratio (SNR) decreases approximately 8.0 dB for a range of arm distances (5-50 cm) between the transmitter and receiver electrodes with constant noise power and various signal amplitudes. The SNR for the 4-PPM scheme is approximately 2 dB higher than that for the 8-PPM one. In addition, the bit error rate (BER) is theoretically analyzed for the human body channel with additive white Gaussian noise. The 4- and 8-PPM IBC systems have average BER values of 10 -5 and 10 -10 , respectively. The results indicate the superiority of the 8-PPM scheme compared to the 4-PPM one when implementing the IBC system. The performance evaluation of the proposed IBC system will improve further IBC transceiver design.

Anbindung des SISIS-SunRise-Bibliothekssystems an das zentrale Identitätsmanagement

NASA Astrophysics Data System (ADS)

Ebner, Ralf; Pretz, Edwin

Wir berichten über Konzepte und Implementierungen zur Datenprovisionierung aus den Personenverwaltungssystemen der Technischen Universität München (TUM) über das zentrale Metadirectory am Leibniz-Rechenzentrum (LRZ) in das SISIS-SunRise-Bibliothekssystem der Universitätsbibliothek der TUM (TUB). Es werden drei Implementierungsvarianten diskutiert, angefangen von der Generierung und Übertragung einfacher CSV-Dateien über ein OpenLDAP-basiertes Konzept als Backend für die SISIS-Datenbank bis zur endgültigen Implementierung mit dem OCLC IDM Connector.

The Governance of the City University of New York A System at Odds with Itself

DTIC Science & Technology

2000-01-01

provided thoughtful and incisive commentary on drafts of this document. Early drafts also benefited from the reviews of task force staff mem - bers...requirements on the qualifications of mem - bers of the board. In particular, the law does not attempt to ensure that trustees have a measure of...establishing a blue-ribbon process for the appointment of the mem - bers of the Board of Trustees. Such a process would involve the cre- ation of

The First Four Years: A Synopsis of the Global Effort. Department of Defense HIV/AIDS Prevention Program (DHAPP)

DTIC Science & Technology

2005-06-01

prevalence esti- mates of approximately 2% in the general population. The Benin Armed Forces (BAF) is composed of approximately 7500 mem - bers, and HIV...Campaigns DHAPP funding sponsored 2 public awareness events for the BAF mem - bers who were deploying to Côte d’Ivoire. Additionally, 38 public awareness...and training programs for service mem - bers, establishing a counseling and testing (CT) program, and improved HIV surveillance and treatment. The

Frequency-noise cancellation in semiconductor lasers by nonlinear heterodyne detection.

PubMed

Bondurant, R S; Welford, D; Alexander, S B; Chan, V W

1986-12-01

The bit-error-rate (BER) performance of conventional noncoherent, heterodyne frequency-shift-keyed (FSK) optical communications systems can be surpassed by the use of a differential FSK modulation format and nonlinear postdetection processing at the receiver. A BER floor exists for conventional frequency-shift keying because of the frequency noise of the transmitter and local oscillator. The use of differential frequency-shift keying with nonlinear postdetection processing suppresses this BER floor for the semiconductor laser system considered here.

Integrating Empirical-Modeling Approaches to Improve Understanding of Terrestrial Ecology Processes

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCarthy, Heather; Luo, Yiqi; Wullschleger, Stan D

Recent decades have seen tremendous increases in the quantity of empirical ecological data collected by individual investigators, as well as through research networks such as FLUXNET (Baldocchi et al., 2001). At the same time, advances in computer technology have facilitated the development and implementation of large and complex land surface and ecological process models. Separately, each of these information streams provides useful, but imperfect information about ecosystems. To develop the best scientific understanding of ecological processes, and most accurately predict how ecosystems may cope with global change, integration of empirical and modeling approaches is necessary. However, true integration - inmore » which models inform empirical research, which in turn informs models (Fig. 1) - is not yet common in ecological research (Luo et al., 2011). The goal of this workshop, sponsored by the Department of Energy, Office of Science, Biological and Environmental Research (BER) program, was to bring together members of the empirical and modeling communities to exchange ideas and discuss scientific practices for increasing empirical - model integration, and to explore infrastructure and/or virtual network needs for institutionalizing empirical - model integration (Yiqi Luo, University of Oklahoma, Norman, OK, USA). The workshop included presentations and small group discussions that covered topics ranging from model-assisted experimental design to data driven modeling (e.g. benchmarking and data assimilation) to infrastructure needs for empirical - model integration. Ultimately, three central questions emerged. How can models be used to inform experiments and observations? How can experimental and observational results be used to inform models? What are effective strategies to promote empirical - model integration?« less

An FPGA design of generalized low-density parity-check codes for rate-adaptive optical transport networks

NASA Astrophysics Data System (ADS)

Zou, Ding; Djordjevic, Ivan B.

2016-02-01

Forward error correction (FEC) is as one of the key technologies enabling the next-generation high-speed fiber optical communications. In this paper, we propose a rate-adaptive scheme using a class of generalized low-density parity-check (GLDPC) codes with a Hamming code as local code. We show that with the proposed unified GLDPC decoder architecture, a variable net coding gains (NCGs) can be achieved with no error floor at BER down to 10-15, making it a viable solution in the next-generation high-speed fiber optical communications.

Reconfigurable WDM-PON empowered by a low-cost 8-channel directly modulated laser module

NASA Astrophysics Data System (ADS)

Zhang, Yi-ming; Liu, Yu; Zhang, Zhi-ke; Zhao, Ze-ping; Tian, Ye; Zhu, Ning-hua

2017-11-01

A 10 Gbit/s 16-km-long reconfigurable wavelength-division-multiplexing passive optical network (WDM-PON) is presented empowered by a low-cost multi-channel directly modulated laser (DML) module. Compared with the case using discrete devices in conventional scheme, the proposed DML module provides a cost-effective solution with reduced complexity. The clear eye diagram and the bit error rate ( BER) of less than 2×10-7 with a sensitivity of -7 dBm are obtained. Due to the special packaging design, the crosstalk between channels under condition of simultaneous operation can be negligible.

Average BER analysis of SCM-based free-space optical systems by considering the effect of IM3 with OSSB signals under turbulence channels.

PubMed

Lim, Wansu; Cho, Tae-Sik; Yun, Changho; Kim, Kiseon

2009-11-09

In this paper, we derive the average bit error rate (BER) of subcarrier multiplexing (SCM)-based free space optics (FSO) systems using a dual-drive Mach-Zehnder modulator (DD-MZM) for optical single-sideband (OSSB) signals under atmospheric turbulence channels. In particular, we consider the third-order intermodulation (IM3), a significant performance degradation factor, in the case of high input signal power systems. The derived average BER, as a function of the input signal power and the scintillation index, is employed to determine the optimum number of SCM users upon the designing FSO systems. For instance, when the user number doubles, the input signal power decreases by almost 2 dBm under the log-normal and exponential turbulence channels at a given average BER.

Decision Fusion with Channel Errors in Distributed Decode-Then-Fuse Sensor Networks

PubMed Central

Yan, Yongsheng; Wang, Haiyan; Shen, Xiaohong; Zhong, Xionghu

2015-01-01

Decision fusion for distributed detection in sensor networks under non-ideal channels is investigated in this paper. Usually, the local decisions are transmitted to the fusion center (FC) and decoded, and a fusion rule is then applied to achieve a global decision. We propose an optimal likelihood ratio test (LRT)-based fusion rule to take the uncertainty of the decoded binary data due to modulation, reception mode and communication channel into account. The average bit error rate (BER) is employed to characterize such an uncertainty. Further, the detection performance is analyzed under both non-identical and identical local detection performance indices. In addition, the performance of the proposed method is compared with the existing optimal and suboptimal LRT fusion rules. The results show that the proposed fusion rule is more robust compared to these existing ones. PMID:26251908

Influence of current pulse shape on directly modulated system performance in metro area optical networks

NASA Astrophysics Data System (ADS)

Campos, Carmina del Rio; Horche, Paloma R.; Martin-Minguez, Alfredo

2011-03-01

Due to the fact that a metro network market is very cost sensitive, direct modulated schemes appear attractive. In this paper a CWDM (Coarse Wavelength Division Multiplexing) system is studied in detail by means of an Optical Communication System Design Software; a detailed study of the modulated current shape (exponential, sine and gaussian) for 2.5 Gb/s CWDM Metropolitan Area Networks is performed to evaluate its tolerance to linear impairments such as signal-to-noise-ratio degradation and dispersion. Point-to-point links are investigated and optimum design parameters are obtained. Through extensive sets of simulation results, it is shown that some of these shape pulses are more tolerant to dispersion when compared with conventional gaussian shape pulses. In order to achieve a low Bit Error Rate (BER), different types of optical transmitters are considered including strongly adiabatic and transient chirp dominated Directly Modulated Lasers (DMLs). We have used fibers with different dispersion characteristics, showing that the system performance depends, strongly, on the chosen DML-fiber couple.

Next-generation bidirectional Triple-play services using RSOA based WDM Radio on Free-Space Optics PON

NASA Astrophysics Data System (ADS)

Mandal, Gour Chandra; Mukherjee, Rahul; Das, Binoy; Patra, Ardhendu Sekhar

2018-03-01

An innovative low cost reflective semiconductor amplifier (RSOA) based bidirectional Triple-play services (TPS) using wavelength division multiplexed radio on free-space-optics passive optical network (WDM-RoFSO-PON) is proposed and experimentally demonstrated to transmit data, voice and video services simultaneously. In this paper, the TPS (10 Gb/s data/voice and 1.49 Gb/s HDTV signal) are successfully transmitted over a 500 m free-space link in downstream and RSOA is utilized at the receiving site to broadcast 1.25 Gb/s data/voice signal over same free-space link in upstream by reusing the carrier, that makes the system cost-effective. High receiver sensitivity and signal-to-noise ratio (SNR), low bit-error-rate (BER) and low error vector magnitude (EVM), and excellent eye-diagrams in our proposed network build the system more reliable and stable with acceptable performance. Therefore, proposed WDM-RoFSO-PON could be the viable solution for future ubiquitous multiservice wireless network in the scenario of TPS.

Partial loss of the DNA repair scaffolding protein, Xrcc1, results in increased brain damage and reduced recovery from ischemic stroke in mice

PubMed Central

Ghosh, Somnath; Canugovi, Chandrika; Yoon, Jeong Seon; Wilson, David M.; Croteau, Deborah L.; Mattson, Mark P.; Bohr, Vilhelm A.

2017-01-01

Oxidative DNA damage is mainly repaired by base excision repair (BER). Previously, our lab showed that mice lacking the BER glycosylases Ogg1 or Neil1 recover more poorly from focal ischemic stroke than wild-type mice. Here, a mouse model was used to investigate whether loss of one of the two alleles of Xrcc1, which encodes a non-enzymatic scaffold protein required for BER, alters recovery from stroke. Ischemia and reperfusion caused higher brain damage and lower functional recovery in Xrcc1+/− mice than in wild-type mice. Additionally, a greater percentage of Xrcc1+/− mice died as a result of the stroke. Brain samples from human individuals who died of stroke and individuals who died of non-neurological causes were assayed for various steps of BER. Significant losses of thymine glycol incision, abasic endonuclease incision and single nucleotide incorporation activities were identified, as well as lower expression of XRCC1 and NEIL1 proteins in stroke brains compared to controls. Together, these results suggest that impaired BER is a risk factor in ischemic brain injury and contributes to its recovery. PMID:25971543

Analytical Evaluation of the Effect of Cross-Polarization-induced Crosstalk on the BER Performance of a PDM-QPSK Coherent Homodyne Optical Transmission System

NASA Astrophysics Data System (ADS)

Taher, K. A.; Majumder, S. P.

2017-05-01

An analytical approach is developed to find the effect of cross-polarization (XPol)-induced crosstalk on the bit error rate (BER) performance of a polarization division multiplex (PDM) quadrature phase shift keying (QPSK) optical transmission system with polarization diversity receiver. Analytical expression for the XPol-induced crosstalk and signal to crosstalk plus noise ratio (SCNR) are developed at the output of polarization diversity PDM-QPSK coherent optical homodyne receiver conditioned on a given value of mean misalignment angle. Considering Maxwellian distribution for the pdf of the misalignment angle, the average SCNR and average BER are derived. Results show that there is significant deterioration in the BER performance and power penalty due to XPol-induced crosstalk. Penalties in signal power are found to be 8.85 dB, 11.28 dB and 12.59 dB correspondingly for LO laser power of -10 dBm, -5 dBm and 0 dBm at a data rate of 100 Gbps, mean misalignment angle of 7.5 degree and BER of 10-9 compared to the signal power without crosstalk.

Real time network traffic monitoring for wireless local area networks based on compressed sensing

NASA Astrophysics Data System (ADS)

Balouchestani, Mohammadreza

2017-05-01

A wireless local area network (WLAN) is an important type of wireless networks which connotes different wireless nodes in a local area network. WLANs suffer from important problems such as network load balancing, large amount of energy, and load of sampling. This paper presents a new networking traffic approach based on Compressed Sensing (CS) for improving the quality of WLANs. The proposed architecture allows reducing Data Delay Probability (DDP) to 15%, which is a good record for WLANs. The proposed architecture is increased Data Throughput (DT) to 22 % and Signal to Noise (S/N) ratio to 17 %, which provide a good background for establishing high qualified local area networks. This architecture enables continuous data acquisition and compression of WLAN's signals that are suitable for a variety of other wireless networking applications. At the transmitter side of each wireless node, an analog-CS framework is applied at the sensing step before analog to digital converter in order to generate the compressed version of the input signal. At the receiver side of wireless node, a reconstruction algorithm is applied in order to reconstruct the original signals from the compressed signals with high probability and enough accuracy. The proposed algorithm out-performs existing algorithms by achieving a good level of Quality of Service (QoS). This ability allows reducing 15 % of Bit Error Rate (BER) at each wireless node.

The NGEE Arctic Data Archive -- Portal for Archiving and Distributing Data and Documentation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boden, Thomas A; Palanisamy, Giri; Devarakonda, Ranjeet

2014-01-01

The Next-Generation Ecosystem Experiments (NGEE Arctic) project is committed to implementing a rigorous and high-quality data management program. The goal is to implement innovative and cost-effective guidelines and tools for collecting, archiving, and sharing data within the project, the larger scientific community, and the public. The NGEE Arctic web site is the framework for implementing these data management and data sharing tools. The open sharing of NGEE Arctic data among project researchers, the broader scientific community, and the public is critical to meeting the scientific goals and objectives of the NGEE Arctic project and critical to advancing the mission ofmore » the Department of Energy (DOE), Office of Science, Biological and Environmental (BER) Terrestrial Ecosystem Science (TES) program.« less

Impairments Computation for Routing Purposes in a Transparent-Access Optical Network Based on Optical CDMA and WDM

NASA Astrophysics Data System (ADS)

Musa, Ahmed

2016-06-01

Optical access networks are becoming more widespread and the use of multiple services might require a transparent optical network (TON). Multiplexing and privacy could benefit from the combination of wavelength division multiplexing (WDM) and optical coding (OC) and wavelength conversion in optical switches. The routing process needs to be cognizant of different resource types and characteristics such as fiber types, fiber linear impairments such as attenuation, dispersion, etc. as well as fiber nonlinear impairments such as four-wave mixing, cross-phase modulation, etc. Other types of impairments, generated by optical nodes or photonic switches, also affect the signal quality (Q) or the optical signal to noise ratio (OSNR), which is related to the bit error rate (BER). Therefore, both link and switch impairments must be addressed and somehow incorporated into the routing algorithm. However, it is not practical to fully integrate all photonic-specific attributes in the routing process. In this study, new routing parameters and constraints are defined that reflect the distinct characteristics of photonic networking. These constraints are applied to the design phase of TON and expressed as a cost or metric form that will be used in the network routing algorithm.

A laboratory system for the investigation of rain fade compensation techniques for Ka-band satellites

NASA Technical Reports Server (NTRS)

Svoboda, James S.; Kachmar, Brian A.

1993-01-01

The design and performance of a rain fade simulation/counteraction system on a laboratory simulated 30/20 GHz, time division multiple access (TDMA) satellite communications testbed is evaluated. Severe rain attenuation of electromagnetic radiation at 30/20 GHz occurs due to the carrier wavelength approaching the water droplet size. Rain in the downlink path lowers the signal power present at the receiver, resulting in a higher number of bit errors induced in the digital ground terminal. The laboratory simulation performed at NASA Lewis Research Center uses a programmable PIN diode attenuator to simulate 20 GHz satellite downlink geographic rain fade profiles. A computer based network control system monitors the downlink power and informs the network of any power threshold violations, which then prompts the network to issue commands that temporarily increase the gain of the satellite based traveling wave tube (TWT) amplifier. After the rain subsides, the network returns the TWT to the normal energy conserving power mode. Bit error rate (BER) data taken at the receiving ground terminal serves as a measure of the severity of rain degradation, and also evaluates the extent to which the network can improve the faded channel.

Calcium partitioning and allocation and blossom-end rot development in tomato plants in response to whole-plant and fruit-specific abscisic acid treatments.

PubMed

Tonetto de Freitas, Sergio; McElrone, Andrew J; Shackel, Kenneth A; Mitcham, Elizabeth J

2014-01-01

The mechanisms regulating Ca(2+) partitioning and allocation in plants and fruit remain poorly understood. The objectives of this study were to determine Ca(2+) partitioning and allocation in tomato plants and fruit in response to whole-plant and fruit-specific abscisic acid (ABA) treatments, as well as to analyse the effect of changes in Ca(2+) partitioning and allocation on fruit susceptibility to the Ca(2+) deficiency disorder blossom-end rot (BER) under water stress conditions. Tomato plants of the cultivar Ace 55 (Vf) were grown in a greenhouse and exposed to low Ca(2+) conditions during fruit growth and development. Starting 1 day after pollination (DAP), the following treatments were initiated: (i) whole plants were sprayed weekly with deionized water (control) or (ii) with 500mg l(-1) ABA; or fruit on each plant were dipped weekly (iii) in deionized water (control) or (iv) in 500mg l(-1) ABA. At 15 DAP, BER was completely prevented by whole-plant or fruit-specific ABA treatments, whereas plants or fruit treated with water had 16-19% BER incidence. At 30 DAP, BER was prevented by the whole-plant ABA treatment, whereas fruit dipped in ABA had a 16% and water-treated plants or fruit had a 36-40% incidence of BER. The results showed that spraying the whole plant with ABA increases xylem sap flow and Ca(2+) movement into the fruit, resulting in higher fruit tissue and water-soluble apoplastic Ca(2+) concentrations that prevent BER development. Although fruit-specific ABA treatment had no effect on xylem sap flow rates or Ca(2+) movement into the fruit, it increased fruit tissue water-soluble apoplastic Ca(2+) concentrations and reduced fruit susceptibility to BER to a lesser extent.

Calcium partitioning and allocation and blossom-end rot development in tomato plants in response to whole-plant and fruit-specific abscisic acid treatments

PubMed Central

Tonetto de Freitas, Sergio

2014-01-01

The mechanisms regulating Ca2+ partitioning and allocation in plants and fruit remain poorly understood. The objectives of this study were to determine Ca2+ partitioning and allocation in tomato plants and fruit in response to whole-plant and fruit-specific abscisic acid (ABA) treatments, as well as to analyse the effect of changes in Ca2+ partitioning and allocation on fruit susceptibility to the Ca2+ deficiency disorder blossom-end rot (BER) under water stress conditions. Tomato plants of the cultivar Ace 55 (Vf) were grown in a greenhouse and exposed to low Ca2+ conditions during fruit growth and development. Starting 1 day after pollination (DAP), the following treatments were initiated: (i) whole plants were sprayed weekly with deionized water (control) or (ii) with 500mg l−1 ABA; or fruit on each plant were dipped weekly (iii) in deionized water (control) or (iv) in 500mg l−1 ABA. At 15 DAP, BER was completely prevented by whole-plant or fruit-specific ABA treatments, whereas plants or fruit treated with water had 16–19% BER incidence. At 30 DAP, BER was prevented by the whole-plant ABA treatment, whereas fruit dipped in ABA had a 16% and water-treated plants or fruit had a 36–40% incidence of BER. The results showed that spraying the whole plant with ABA increases xylem sap flow and Ca2+ movement into the fruit, resulting in higher fruit tissue and water-soluble apoplastic Ca2+ concentrations that prevent BER development. Although fruit-specific ABA treatment had no effect on xylem sap flow rates or Ca2+ movement into the fruit, it increased fruit tissue water-soluble apoplastic Ca2+ concentrations and reduced fruit susceptibility to BER to a lesser extent. PMID:24220654

SITE project. Phase 1: Continuous data bit-error-rate testing

NASA Technical Reports Server (NTRS)

Fujikawa, Gene; Kerczewski, Robert J.

1992-01-01

The Systems Integration, Test, and Evaluation (SITE) Project at NASA LeRC encompasses a number of research and technology areas of satellite communications systems. Phase 1 of this project established a complete satellite link simulator system. The evaluation of proof-of-concept microwave devices, radiofrequency (RF) and bit-error-rate (BER) testing of hardware, testing of remote airlinks, and other tests were performed as part of this first testing phase. This final report covers the test results produced in phase 1 of the SITE Project. The data presented include 20-GHz high-power-amplifier testing, 30-GHz low-noise-receiver testing, amplitude equalization, transponder baseline testing, switch matrix tests, and continuous-wave and modulated interference tests. The report also presents the methods used to measure the RF and BER performance of the complete system. Correlations of the RF and BER data are summarized to note the effects of the RF responses on the BER.

Orthogonal Multi-Carrier DS-CDMA with Frequency-Domain Equalization

NASA Astrophysics Data System (ADS)

Tanaka, Ken; Tomeba, Hiromichi; Adachi, Fumiyuki

Orthogonal multi-carrier direct sequence code division multiple access (orthogonal MC DS-CDMA) is a combination of orthogonal frequency division multiplexing (OFDM) and time-domain spreading, while multi-carrier code division multiple access (MC-CDMA) is a combination of OFDM and frequency-domain spreading. In MC-CDMA, a good bit error rate (BER) performance can be achieved by using frequency-domain equalization (FDE), since the frequency diversity gain is obtained. On the other hand, the conventional orthogonal MC DS-CDMA fails to achieve any frequency diversity gain. In this paper, we propose a new orthogonal MC DS-CDMA that can obtain the frequency diversity gain by applying FDE. The conditional BER analysis is presented. The theoretical average BER performance in a frequency-selective Rayleigh fading channel is evaluated by the Monte-Carlo numerical computation method using the derived conditional BER and is confirmed by computer simulation of the orthogonal MC DS-CDMA signal transmission.

Oxidized nucleotide insertion by pol β confounds ligation during base excision repair

PubMed Central

Çağlayan, Melike; Horton, Julie K.; Dai, Da-Peng; Stefanick, Donna F.; Wilson, Samuel H.

2017-01-01

Oxidative stress in cells can lead to accumulation of reactive oxygen species and oxidation of DNA precursors. Oxidized purine nucleotides can be inserted into DNA during replication and repair. The main pathway for correcting oxidized bases in DNA is base excision repair (BER), and in vertebrates DNA polymerase β (pol β) provides gap filling and tailoring functions. Here we report that the DNA ligation step of BER is compromised after pol β insertion of oxidized purine nucleotides into the BER intermediate in vitro. These results suggest the possibility that BER mediated toxic strand breaks are produced in cells under oxidative stress conditions. We observe enhanced cytotoxicity in oxidizing-agent treated pol β expressing mouse fibroblasts, suggesting formation of DNA strand breaks under these treatment conditions. Increased cytotoxicity following MTH1 knockout or treatment with MTH1 inhibitor suggests the oxidation of precursor nucleotides. PMID:28067232

Bildanalyse in Medizin und Biologie

NASA Astrophysics Data System (ADS)

Athelogou, Maria; Schönmeyer, Ralf; Schmidt, Günther; Schäpe, Arno; Baatz, Martin; Binnig, Gerd

Heutzutage sind bildgebende Verfahren aus medizinischen Untersuchungen nicht mehr wegzudenken. Diverse Methoden - basierend auf dem Einsatz von Ultraschallwellen, Röntgenstrahlung, Magnetfeldern oder Lichtstrahlen - werden dabei spezifisch eingesetzt und liefern umfangreiches Datenmaterial über den Körper und sein Inneres. Anhand von Mikroskopieaufnahmen aus Biopsien können darüber hinaus Daten über die morphologische Eigenschaften von Körpergeweben gewonnen werden. Aus der Analyse all dieser unterschiedlichen Arten von Informationen und unter Konsultation weiterer klinischer Untersuchungen aus diversen medizinischen Disziplinen kann unter Berücksichtigung von Anamnesedaten ein "Gesamtbild“ des Gesundheitszustands eines Patienten erstellt werden. Durch die Flut der erzeugten Bilddaten kommt der Bildverarbeitung im Allgemeinen und der Bildanalyse im Besonderen eine immer wichtigere Rolle zu. Gerade im Bereich der Diagnoseunterstützung, der Therapieplanung und der bildgeführten Chirurgie bilden sie Schlüsseltechnologien, die den Forschritt nicht nur auf diesen Gebieten maßgeblich vorantreiben.

Adaptive software-defined coded modulation for ultra-high-speed optical transport

NASA Astrophysics Data System (ADS)

Djordjevic, Ivan B.; Zhang, Yequn

2013-10-01

In optically-routed networks, different wavelength channels carrying the traffic to different destinations can have quite different optical signal-to-noise ratios (OSNRs) and signal is differently impacted by various channel impairments. Regardless of the data destination, an optical transport system (OTS) must provide the target bit-error rate (BER) performance. To provide target BER regardless of the data destination we adjust the forward error correction (FEC) strength. Depending on the information obtained from the monitoring channels, we select the appropriate code rate matching to the OSNR range that current channel OSNR falls into. To avoid frame synchronization issues, we keep the codeword length fixed independent of the FEC code being employed. The common denominator is the employment of quasi-cyclic (QC-) LDPC codes in FEC. For high-speed implementation, low-complexity LDPC decoding algorithms are needed, and some of them will be described in this invited paper. Instead of conventional QAM based modulation schemes, we employ the signal constellations obtained by optimum signal constellation design (OSCD) algorithm. To improve the spectral efficiency, we perform the simultaneous rate adaptation and signal constellation size selection so that the product of number of bits per symbol × code rate is closest to the channel capacity. Further, we describe the advantages of using 4D signaling instead of polarization-division multiplexed (PDM) QAM, by using the 4D MAP detection, combined with LDPC coding, in a turbo equalization fashion. Finally, to solve the problems related to the limited bandwidth of information infrastructure, high energy consumption, and heterogeneity of optical networks, we describe an adaptive energy-efficient hybrid coded-modulation scheme, which in addition to amplitude, phase, and polarization state employs the spatial modes as additional basis functions for multidimensional coded-modulation.

Base excision repair imbalance in colorectal cancer has prognostic value and modulates response to chemotherapy

PubMed Central

Leguisamo, Natalia M.; Gloria, Helena C.; Kalil, Antonio N.; Martins, Talita V.; Azambuja, Daniel B.

2017-01-01

Colorectal cancer (CRC) is prevalent worldwide, and treatment often involves surgery and genotoxic chemotherapy. DNA repair mechanisms, such as base excision repair (BER) and mismatch repair (MMR), may not only influence tumour characteristics and prognosis but also dictate chemotherapy response. Defective MMR contributes to chemoresistance in colorectal cancer. Moreover, BER affects cellular survival by repairing genotoxic base damage in a process that itself can disrupt metabolism. In this study, we characterized BER and MMR gene expression in colorectal tumours and the association between this repair profile with patients’ clinical and pathological features. In addition, we exploited the possible mechanisms underlying the association between altered DNA repair, metabolism and response to chemotherapy. Seventy pairs of sporadic colorectal tumour samples and adjacent non-tumour mucosal specimens were assessed for BER and MMR gene and protein expression and their association with pathological and clinical features. MMR-deficient colon cancer cells (HCT116) transiently overexpressing MPG or XRCC1 were treated with 5-FU or TMZ and evaluated for viability and metabolic intermediate levels. Increase in BER gene and protein expression is associated with more aggressive tumour features and poor pathological outcomes in CRC. However, tumours with reduced MMR gene expression also displayed low MPG, OGG1 and PARP1 expression. Imbalancing BER by overexpression of MPG, but not XRCC1, sensitises MMR-deficient colon cancer cells to 5-FU and TMZ and leads to ATP depletion and lactate accumulation. MPG overexpression alters DNA repair and metabolism and is a potential strategy to overcome 5-FU chemotherapeutic resistance in MMR-deficient CRC. PMID:28903334

Inhibitory Mechanisms of Human CYPs by Three Alkaloids Isolated from Traditional Chinese Herbs.

PubMed

Zhao, Yong; Hellum, Bent Håvard; Liang, Aihua; Nilsen, Odd Georg

2015-06-01

The three purified herbal compounds tetrahydropalmatine (Tet), neferine and berberine (Ber) were explored in vitro for basic inhibition mechanisms towards recombinant human CYP1A2, CYP2D6 and CYP3A4 metabolic activities. Phenacetin, dextromethorphan and testosterone, respectively, were used as CYP1A2, CYP2D6 and CYP3A4 substrates, and their metabolites were determined by validated HPLC methodologies. Positive inhibition controls were used. Mechanism-based (irreversible) inhibition was assessed by time-dependent and nicotinamide adenine dinucleotide phosphate-dependent and reversible inhibition by Lineweaver-Burk plot assessments. Inhibition mechanisms were also assessed by computerized interaction prediction by using the Discovery Studio CDOCKER software (Accelrys, San Diego, CA, USA). Tetrahydropalmatine showed a mechanism-based inhibition of both CYP1A2 and CYP2D6, and Ber of CYP2D6. Neferine and Ber both showed a nonmechanistic inhibition of CYP1A2. All compounds showed a similar and significant mechanism-based inhibition of CYP3A4. Tetrahydropalmatine and Ber demonstrated both reversible and irreversible inhibition of CYP2D6 and CYP3A4. Tetrahydropalmatine and Ber displayed H-bond and several Pi-bond connections with specific amino acid residues of CYP1A2, CYP2D6 and CYP3A4, giving further knowledge to the identified reversible and irreversible herb-drug interactions. Tetrahydropalmatine and Ber should be considered for herb-drug interactions in clinical therapy until relevant clinical studies are available. Copyright © 2015 John Wiley & Sons, Ltd.

Nucleosomes suppress the formation of double-strand DNA breaks during attempted base excision repair of clustered oxidative damages.

PubMed

Cannan, Wendy J; Tsang, Betty P; Wallace, Susan S; Pederson, David S

2014-07-18

Exposure to ionizing radiation can produce multiple, clustered oxidative lesions in DNA. The near simultaneous excision of nearby lesions in opposing DNA strands by the base excision repair (BER) enzymes can produce double-strand DNA breaks (DSBs). This attempted BER accounts for many of the potentially lethal or mutagenic DSBs that occur in vivo. To assess the impact of nucleosomes on the frequency and pattern of BER-dependent DSB formation, we incubated nucleosomes containing oxidative damages in opposing DNA strands with selected DNA glycosylases and human apurinic/apyrimidinic endonuclease 1. Overall, nucleosomes substantially suppressed DSB formation. However, the degree of suppression varied as a function of (i) the lesion type and DNA glycosylase tested, (ii) local sequence context and the stagger between opposing strand lesions, (iii) the helical orientation of oxidative lesions relative to the underlying histone octamer, and (iv) the distance between the lesion cluster and the nucleosome edge. In some instances the binding of a BER factor to one nucleosomal lesion appeared to facilitate binding to the opposing strand lesion. DSB formation did not invariably lead to nucleosome dissolution, and in some cases, free DNA ends resulting from DSB formation remained associated with the histone octamer. These observations explain how specific structural and dynamic properties of nucleosomes contribute to the suppression of BER-generated DSBs. These studies also suggest that most BER-generated DSBs will occur in linker DNA and in genomic regions associated with elevated rates of nucleosome turnover or remodeling. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Nucleosomes Suppress the Formation of Double-strand DNA Breaks during Attempted Base Excision Repair of Clustered Oxidative Damages*

PubMed Central

Cannan, Wendy J.; Tsang, Betty P.; Wallace, Susan S.; Pederson, David S.

2014-01-01

Exposure to ionizing radiation can produce multiple, clustered oxidative lesions in DNA. The near simultaneous excision of nearby lesions in opposing DNA strands by the base excision repair (BER) enzymes can produce double-strand DNA breaks (DSBs). This attempted BER accounts for many of the potentially lethal or mutagenic DSBs that occur in vivo. To assess the impact of nucleosomes on the frequency and pattern of BER-dependent DSB formation, we incubated nucleosomes containing oxidative damages in opposing DNA strands with selected DNA glycosylases and human apurinic/apyrimidinic endonuclease 1. Overall, nucleosomes substantially suppressed DSB formation. However, the degree of suppression varied as a function of (i) the lesion type and DNA glycosylase tested, (ii) local sequence context and the stagger between opposing strand lesions, (iii) the helical orientation of oxidative lesions relative to the underlying histone octamer, and (iv) the distance between the lesion cluster and the nucleosome edge. In some instances the binding of a BER factor to one nucleosomal lesion appeared to facilitate binding to the opposing strand lesion. DSB formation did not invariably lead to nucleosome dissolution, and in some cases, free DNA ends resulting from DSB formation remained associated with the histone octamer. These observations explain how specific structural and dynamic properties of nucleosomes contribute to the suppression of BER-generated DSBs. These studies also suggest that most BER-generated DSBs will occur in linker DNA and in genomic regions associated with elevated rates of nucleosome turnover or remodeling. PMID:24891506

The role of the PHP domain associated with DNA polymerase X from Thermus thermophilus HB8 in base excision repair.

PubMed

Nakane, Shuhei; Nakagawa, Noriko; Kuramitsu, Seiki; Masui, Ryoji

2012-11-01

Base excision repair (BER) is one of the most commonly used DNA repair pathways involved in genome stability. X-family DNA polymerases (PolXs) play critical roles in BER, especially in filling single-nucleotide gaps. In addition to a polymerase core domain, bacterial PolXs have a polymerase and histidinol phosphatase (PHP) domain with phosphoesterase activity which is also required for BER. However, the role of the PHP domain of PolX in bacterial BER remains unresolved. We found that the PHP domain of Thermus thermophilus HB8 PolX (ttPolX) functions as two types of phosphoesterase in BER, including a 3'-phosphatase and an apurinic/apyrimidinic (AP) endonuclease. Experiments using T. thermophilus HB8 cell lysates revealed that the majority of the 3'-phosphatase and AP endonuclease activities are attributable to the another phosphoesterase in T. thermophilus HB8, endonuclease IV (ttEndoIV). However, ttPolX possesses significant 3'-phosphatase activity in ΔttendoIV cell lysate, indicating possible complementation. Our experiments also reveal that there are only two enzymes that display the 3'-phosphatase activity in the T. thermophilus HB8 cell, ttPolX and ttEndoIV. Furthermore, phenotypic analysis of ΔttpolX, ΔttendoIV, and ΔttpolX/ΔttendoIV using hydrogen peroxide and sodium nitrite supports the hypothesis that ttPolX functions as a backup for ttEndoIV in BER. Copyright © 2012 Elsevier B.V. All rights reserved.

Genome-wide map of Apn1 binding sites under oxidative stress in Saccharomyces cerevisiae.

PubMed

Morris, Lydia P; Conley, Andrew B; Degtyareva, Natalya; Jordan, I King; Doetsch, Paul W

2017-11-01

The DNA is cells is continuously exposed to reactive oxygen species resulting in toxic and mutagenic DNA damage. Although the repair of oxidative DNA damage occurs primarily through the base excision repair (BER) pathway, the nucleotide excision repair (NER) pathway processes some of the same lesions. In addition, damage tolerance mechanisms, such as recombination and translesion synthesis, enable cells to tolerate oxidative DNA damage, especially when BER and NER capacities are exceeded. Thus, disruption of BER alone or disruption of BER and NER in Saccharomyces cerevisiae leads to increased mutations as well as large-scale genomic rearrangements. Previous studies demonstrated that a particular region of chromosome II is susceptible to chronic oxidative stress-induced chromosomal rearrangements, suggesting the existence of DNA damage and/or DNA repair hotspots. Here we investigated the relationship between oxidative damage and genomic instability utilizing chromatin immunoprecipitation combined with DNA microarray technology to profile DNA repair sites along yeast chromosomes under different oxidative stress conditions. We targeted the major yeast AP endonuclease Apn1 as a representative BER protein. Our results indicate that Apn1 target sequences are enriched for cytosine and guanine nucleotides. We predict that BER protects these sites in the genome because guanines and cytosines are thought to be especially susceptible to oxidative attack, thereby preventing large-scale genome destabilization from chronic accumulation of DNA damage. Information from our studies should provide insight into how regional deployment of oxidative DNA damage management systems along chromosomes protects against large-scale rearrangements. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

Isotopic nitrogen in fecal fiber as an indicator of winter diet in caribou and muskoxen

USGS Publications Warehouse

Gustine, David D.; Barboza, Perry S.; Addison, Jennifer; Shively, Rachel; Oliver, Lola

2014-01-01

RATIONALE: The ratios of stable nitrogen isotopes (δ15N values) in excreta have been used to examine aspects of trophic and nutritional ecology across taxa. Nitrogen fractions in feces of herbivores include endogenous (e.g., sloughed intestinal cells, unresorbed digestive secretions, and microbial debris) and dietary sources. For animals such as large herbivores, that have diets and feces with high concentrations of indigestible fiber, endogenous 15N may constrain the use of fecal δ15N values to estimate dietary δ15N values and reconstruct diets. METHODS: We compared two techniques (detergent and detergent-free) to isolate fractions of plant fibers in the forages of caribou (Rangifer tarandus) and muskoxen (Ovibos moschatus); estimated the discrimination factors between the δ15N values of fecal fiber residues and of the diets of captive animals; and used the more effective isotopic tracer of dietary δ15N values to examine the relationships between the δ15N values of fecal residues and diet composition in several populations of wild caribou and muskoxen throughout North America in winter. RESULTS: The detergent-based approach contaminated the fractions of plant fibers in forages and feces with 14N, whereas the detergent-free method was a good proxy to estimate δ15N values of plant fibers (r2= 0.92) and provided a better estimate of the fecal-fiber to diet discrimination factor for both species (caribou = 3.6‰; muskoxen = 2.8‰). In wild populations, the δ15N values of fecal fibers reflected diet composition in muskoxen (adjusted R2= 0.43) but not caribou (adjusted R2= 0.06). CONCLUSIONS: Contamination from detergent residues prohibited the use of detergent extraction in isolating forage 15N from endogenous 15N in the feces of herbivores. Although δ15N values in fecal fibers can be used to track dietary δ15N values in wild herbivores, discrimination between fecal extracts and diet may vary with the contribution of endogenous nitrogen (N), and, therefore, residual endogenous 15N in feces may limit dietary reconstructions from fecal δ15N values for some large herbivores.

An 802.11 n wireless local area network transmission scheme for wireless telemedicine applications.

PubMed

Lin, C F; Hung, S I; Chiang, I H

2010-10-01

In this paper, an 802.11 n transmission scheme is proposed for wireless telemedicine applications. IEEE 802.11n standards, a power assignment strategy, space-time block coding (STBC), and an object composition Petri net (OCPN) model are adopted. With the proposed wireless system, G.729 audio bit streams, Joint Photographic Experts Group 2000 (JPEG 2000) clinical images, and Moving Picture Experts Group 4 (MPEG-4) video bit streams achieve a transmission bit error rate (BER) of 10-7, 10-4, and 103 simultaneously. The proposed system meets the requirements prescribed for wireless telemedicine applications. An essential feature of this proposed transmission scheme is that clinical information that requires a high quality of service (QoS) is transmitted at a high power transmission rate with significant error protection. For maximizing resource utilization and minimizing the total transmission power, STBC and adaptive modulation techniques are used in the proposed 802.11 n wireless telemedicine system. Further, low power, direct mapping (DM), low-error protection scheme, and high-level modulation are adopted for messages that can tolerate a high BER. With the proposed transmission scheme, the required reliability of communication can be achieved. Our simulation results have shown that the proposed 802.11 n transmission scheme can be used for developing effective wireless telemedicine systems.

High-speed laser communications in UAV scenarios

NASA Astrophysics Data System (ADS)

Griethe, Wolfgang; Gregory, Mark; Heine, Frank; Kämpfner, Hartmut

2011-05-01

Optical links, based on coherent homodyne detection and BPSK modulation with bidirectional data transmission of 5.6 Gbps over distances of about 5,000 km and BER of 10-8, have been sufficiently verified in space. The verification results show that this technology is suitable not only for space applications but also for applications in the troposphere. After a brief description of the Laser Communication Terminal (LCT) for space applications, the paper consequently discusses the future utilization of satellite-based optical data links for Beyond Line of Sight (BLOS) operations of High Altitude Long Endurance (HALE) Unmanned Aerial Vehicles (UAV). It is shown that the use of optical frequencies is the only logical consequence of an ever-increasing demand for bandwidth. In terms of Network Centric Warfare it is highly recommended that Unmanned Aircraft Systems (UAS) of the future should incorporate that technology which allows almost unlimited bandwidth. The advantages of optical communications especially for Intelligence, Surveillance and Reconnaissance (ISR) are underlined. Moreover, the preliminary design concept of an airborne laser communication terminal is described. Since optical bi-directional links have been tested between a LCT in space and a TESAT Optical Ground Station (OGS), preliminary analysis on tracking and BER performance and the impact of atmospheric disturbances on coherent links will be presented.

Exploring crosstalk noise generated in the N-port router used in the WDM-based ONoC

NASA Astrophysics Data System (ADS)

Zhang, Zhendong; Xie, Yiyuan; Song, Tingting; He, Chao; Li, Jiachao; Liu, Yong

2017-07-01

Compared with optical network-on-chip (ONoC) with single wavelength, ONoC adopting wavelength division multiplexing (WDM) technology possesses a very prominent advantage-higher bandwidth. Therefore, WDM-based ONoC has been considered one of the most promising ways to relieve the rapidly increasing traffic load in communication systems. A WDM-based router, as the core equipment of WDM-based ONoC, is influenced by crosstalk noise, especially the nonlinear crosstalk noise generated by the four-wave mixing effect. Thus, to explore the performance of the N-port nonblocking optical router using WDM, we propose a universal analytic model to analyze the transmission loss, crosstalk noise, optical signal-to-noise ratio (OSNR), and bit error ratio (BER). The research results show that crosstalk noise varies along with signals at different wavelengths in the same channel. For signals with the same wavelength, the noises generated in the different transmission paths are obviously different from each other. For research of transmission loss, OSNR, and BER, similar results can be obtained. Based on the eye diagrams, we can learn that crosstalk noise will cause signal distortion to a certain extent. With this model, capability of this kind of multiport optical router using WDM can be understood conveniently.

Revisiting regression in autism: Heller's dementia infantilis. Includes a translation of Über Dementia Infantilis.

PubMed

Westphal, Alexander; Schelinski, Stefanie; Volkmar, Fred; Pelphrey, Kevin

2013-02-01

Theodor Heller first described a severe regression of adaptive function in normally developing children, something he termed dementia infantilis, over one 100 years ago. Dementia infantilis is most closely related to the modern diagnosis, childhood disintegrative disorder. We translate Heller's paper, Über Dementia Infantilis, and discuss similarities in presentation between Heller's cases, and a group of children with childhood disintegrative disorder. In particular we discuss a prodromal period of affective dysregulation described by Heller, and also evident in our sample, but not previously described in any detail since the publication of Über Dementia Infantilis.

Bit error rate performance of Image Processing Facility high density tape recorders

NASA Technical Reports Server (NTRS)

Heffner, P.

1981-01-01

The Image Processing Facility at the NASA/Goddard Space Flight Center uses High Density Tape Recorders (HDTR's) to transfer high volume image data and ancillary information from one system to another. For ancillary information, it is required that very low bit error rates (BER's) accompany the transfers. The facility processes about 10 to the 11th bits of image data per day from many sensors, involving 15 independent processing systems requiring the use of HDTR's. When acquired, the 16 HDTR's offered state-of-the-art performance of 1 x 10 to the -6th BER as specified. The BER requirement was later upgraded in two steps: (1) incorporating data randomizing circuitry to yield a BER of 2 x 10 to the -7th and (2) further modifying to include a bit error correction capability to attain a BER of 2 x 10 to the -9th. The total improvement factor was 500 to 1. Attention is given here to the background, technical approach, and final results of these modifications. Also discussed are the format of the data recorded by the HDTR, the magnetic tape format, the magnetic tape dropout characteristics as experienced in the Image Processing Facility, the head life history, and the reliability of the HDTR's.

Remote heterodyne millimeter-wave over fiber based OFDM-PON with master-to-slave injected dual-mode colorless FPLD pair.

PubMed

Chen, Hsiang-Yu; Chi, Yu-Chieh; Lin, Gong-Ru

2015-08-24

A remote heterodyne millimeter-wave (MMW) carrier at 47.7 GHz over fiber synthesized with the master-to-slave injected dual-mode colorless FPLD pair is proposed, which enables the future connection between the wired fiber-optic 64-QAM OFDM-PON at 24 Gb/s with the MMW 4-QAM OFDM wireless network at 2 Gb/s. Both the single- and dual-mode master-to-slave injection-locked colorless FPLD pairs are compared to optimize the proposed 64-QAM OFDM-PON. For the unamplified single-mode master, the slave colorless FPLD successfully performs the 64-QAM OFDM data at 24 Gb/s with EVM, SNR and BER of 8.5%, 21.5 dB and 2.9 × 10(-3), respectively. In contrast, the dual-mode master-to-slave injection-locked colorless FPLD pair with amplified and unfiltered master can transmit 64-QAM OFDM data at 18 Gb/s over 25-km SMF to provide EVM, SNR and BER of 8.2%, 21.8 dB and 2.2 × 10(-3), respectively. For the dual-mode master-to-slave injection-locked colorless FPLD pair, even though the modal dispersion occurred during 25-km SMF transmission makes it sacrifice the usable OFDM bandwidth by only 1 GHz, which guarantees the sufficient encoding bitrate for the optically generated MMW carrier to implement the fusion of MMW wireless LAN and DWDM-PON with cost-effective and compact architecture. As a result, the 47.7-GHz MMW carrier remotely beat from the dual-mode master-to-slave injection-locked colorless FPLD pair exhibits an extremely narrow bandwidth of only 0.48 MHz. After frequency down-conversion operation, the 47.7-GHz MMW carrier successfully delivers 4-QAM OFDM data up to 2 Gb/s with EVM, SNR and BER of 33.5%, 9.51 dB and 1.4 × 10(-3), respectively.

Polymer enabled 100 Gbaud connectivity for datacom applications

NASA Astrophysics Data System (ADS)

Katopodis, V.; Groumas, P.; Zhang, Z.; Dinu, R.; Miller, E.; Konczykowska, A.; Dupuy, J.-Y.; Beretta, A.; Dede, A.; Choi, J. H.; Harati, P.; Jorge, F.; Nodjiadjim, V.; Riet, Muriel; Cangini, G.; Vannucci, A.; Keil, N.; Bach, H.-G.; Grote, N.; Avramopoulos, H.; Kouloumentas, Ch.

2016-03-01

Polymers hold the promise for ultra-fast modulation of optical signals due to their potential for ultra-fast electro-optic (EO) response and high EO coefficient. In this work, we present the basic structure and properties of an efficient EO material system, and we summarize the efforts made within the project ICT-POLYSYS for the development of high-speed transmitters based on this system. More specifically, we describe successful efforts for the monolithic integration of multi-mode interference (MMI) couplers and Bragg-gratings (BGs) along with Mach-Zehnder modulators (MZMs) on this platform, and for the hybrid integration of InP active elements in the form of laser diodes (LDs) and gain chips (GCs). Using these integration techniques and the combination of the hybrid optical chips with ultra-fast indium phosphide double heterojunction bipolar transistor (InP-DHBT) electronics, we develop and fully package a single 100 Gb/s transmitter and a 2×100 Gb/s transmitter that can support serial operation at this rate with conventional non-return-to-zero on-off-keying (NRZ-OOK) modulation format. We also present the experimental evaluation of the devices, validating the efficiency of the monolithic and hybrid integration concepts and confirming the potential of this technology for single-lane 100 Gb/s optical connectivity in data-center network environments. Results from transmission experiments to this end include the achievement of BER close to 6·10-9 in B2B configuration, the achievement of BER lower than 10-7 for propagation over standard single-mode fiber (SSMF) with total length up to 1000 m, and the achievement of BER at the level of 10-5 after 1625 m of SSMF. Finally, plans for the use of the EO polymer system in a more complex hybrid integration platform for high-flexibility/high-capacity transmitters are also outlined.

Performance of the hybrid MLPNN based VE (hMLPNN-VE) for the nonlinear PMR channels

NASA Astrophysics Data System (ADS)

Wongsathan, Rati; Phakphisut, Watid; Supnithi, Pornchai

2018-05-01

This paper proposes a hybrid of multilayer perceptron neural network (MLPNN) and Volterra equalizer (VE) denoted hMLPNN-VE in nonlinear perpendicular magnetic recording (PMR) channels. The proposed detector integrates the nonlinear product terms of the delayed readback signals generated from the VE into the nonlinear processing of the MLPNN. The detection performance comparison is evaluated in terms of the tradeoff between the bit error rate (BER), complexity and reliability for a nonlinear Volterra channel at high normalized recording density. The proposed hMLPNN-VE outperforms MLPNN based equalizer (MLPNNE), VE and the conventional partial response maximum likelihood (PRML) detector.

Analysis of physical layer performance of data center with optical wavelength switches based on advanced modulation formats

NASA Astrophysics Data System (ADS)

Ahmad, Iftikhar; Chughtai, Mohsan Niaz

2018-05-01

In this paper the IRIS (Integrated Router Interconnected spectrally), an optical domain architecture for datacenter network is analyzed. The IRIS integrated with advanced modulation formats (M-QAM) and coherent optical receiver is analyzed. The channel impairments are compensated using the DSP algorithms following the coherent receiver. The proposed scheme allows N2 multiplexed wavelengths for N×N size. The performance of the N×N-IRIS switch with and without wavelength conversion is analyzed for different Baud rates over M-QAM modulation formats. The performance of the system is analyzed in terms of bit error rate (BER) vs OSNR curves.

Performance analysis of communication links based on VCSEL and silicon photonics technology for high-capacity data-intensive scenario.

PubMed

Boletti, A; Boffi, P; Martelli, P; Ferrario, M; Martinelli, M

2015-01-26

To face the increased demand for bandwidth, cost-effectiveness and simplicity of future Ethernet data communications, a comparison between two different solutions based on directly-modulated VCSEL sources and Silicon Photonics technologies is carried out. Also by exploiting 4-PAM modulation, the transmission of 50-Gb/s and beyond capacity per channel is analyzed by means of BER performance. Applications for optical backplane, very short reach and in case of client-optics networks and intra and inter massive data centers communications (up to 10 km) are taken into account. A comparative analysis based on the power consumption is also proposed.

Polarization-multiplexed rate-adaptive non-binary-quasi-cyclic-LDPC-coded multilevel modulation with coherent detection for optical transport networks.

PubMed

Arabaci, Murat; Djordjevic, Ivan B; Saunders, Ross; Marcoccia, Roberto M

2010-02-01

In order to achieve high-speed transmission over optical transport networks (OTNs) and maximize its throughput, we propose using a rate-adaptive polarization-multiplexed coded multilevel modulation with coherent detection based on component non-binary quasi-cyclic (QC) LDPC codes. Compared to prior-art bit-interleaved LDPC-coded modulation (BI-LDPC-CM) scheme, the proposed non-binary LDPC-coded modulation (NB-LDPC-CM) scheme not only reduces latency due to symbol- instead of bit-level processing but also provides either impressive reduction in computational complexity or striking improvements in coding gain depending on the constellation size. As the paper presents, compared to its prior-art binary counterpart, the proposed NB-LDPC-CM scheme addresses the needs of future OTNs, which are achieving the target BER performance and providing maximum possible throughput both over the entire lifetime of the OTN, better.

A WDM-PON with DPSK modulated downstream and OOK modulated upstream signals based on symmetric 10 Gbit/s wavelength reused bidirectional reflective SOA

NASA Astrophysics Data System (ADS)

El-Nahal, Fady I.

2017-01-01

We investigate a wavelength-division-multiplexing passive optical network (WDM-PON) with centralized lightwave and direct detection. The system is demonstrated for symmetric 10 Gbit/s differential phase-shift keying (DPSK) downstream signals and on-off keying (OOK) upstream signals, respectively. A wavelength reused scheme is employed to carry the upstream data by using a reflective semiconductor optical amplifier (RSOA) as an intensity modulator at the optical network unit (ONU). The constant-intensity property of the DPSK modulation format can keep high extinction ratio ( ER) of downstream signal and reduce the crosstalk to the upstream signal. The bit error rate ( BER) performance of our scheme shows that the proposed 10 Gbit/s symmetric WDM-PON can achieve error free transmission over 25-km-long fiber transmission with low power penalty.

Transmission of 2.5 Gbit/s Spectrum-sliced WDM System for 50 km Single-mode Fiber

NASA Astrophysics Data System (ADS)

Ahmed, Nasim; Aljunid, Sayed Alwee; Ahmad, R. Badlisha; Fadil, Hilal Adnan; Rashid, Mohd Abdur

2011-06-01

The transmission of a spectrum-sliced WDM channel at 2.5 Gbit/s for 50 km of single mode fiber using an system channel spacing only 0.4 nm is reported. We have investigated the system performance using NRZ modulation format. The proposed system is compared with conventional system. The system performance is characterized as the bit-error-rate (BER) received against the system bit rates. Simulation results show that the NRZ modulation format performs well for 2.5 Gbit/s system bit rates. Using this narrow channel spectrum-sliced technique, the total number of multiplexed channels can be increased greatly in WDM system. Therefore, 0.4 nm channel spacing spectrum-sliced WDM system is highly recommended for the long distance optical access networks, like the Metro Area Network (MAN), Fiber-to-the-Building (FTTB) and Fiber-to-the-Home (FTTH).

Performance Analysis of Diversity-Controlled Multi-User Superposition Transmission for 5G Wireless Networks

PubMed Central

Yeom, Jeong Seon; Jung, Bang Chul; Jin, Hu

2018-01-01

In this paper, we propose a novel low-complexity multi-user superposition transmission (MUST) technique for 5G downlink networks, which allows multiple cell-edge users to be multiplexed with a single cell-center user. We call the proposed technique diversity-controlled MUST technique since the cell-center user enjoys the frequency diversity effect via signal repetition over multiple orthogonal frequency division multiplexing (OFDM) sub-carriers. We assume that a base station is equipped with a single antenna but users are equipped with multiple antennas. In addition, we assume that the quadrature phase shift keying (QPSK) modulation is used for users. We mathematically analyze the bit error rate (BER) of both cell-edge users and cell-center users, which is the first theoretical result in the literature to the best of our knowledge. The mathematical analysis is validated through extensive link-level simulations. PMID:29439413

Performance Analysis of Diversity-Controlled Multi-User Superposition Transmission for 5G Wireless Networks.

PubMed

Yeom, Jeong Seon; Chu, Eunmi; Jung, Bang Chul; Jin, Hu

2018-02-10

In this paper, we propose a novel low-complexity multi-user superposition transmission (MUST) technique for 5G downlink networks, which allows multiple cell-edge users to be multiplexed with a single cell-center user. We call the proposed technique diversity-controlled MUST technique since the cell-center user enjoys the frequency diversity effect via signal repetition over multiple orthogonal frequency division multiplexing (OFDM) sub-carriers. We assume that a base station is equipped with a single antenna but users are equipped with multiple antennas. In addition, we assume that the quadrature phase shift keying (QPSK) modulation is used for users. We mathematically analyze the bit error rate (BER) of both cell-edge users and cell-center users, which is the first theoretical result in the literature to the best of our knowledge. The mathematical analysis is validated through extensive link-level simulations.

NB-PLC channel modelling with cyclostationary noise addition & OFDM implementation for smart grid

NASA Astrophysics Data System (ADS)

Thomas, Togis; Gupta, K. K.

2016-03-01

Power line communication (PLC) technology can be a viable solution for the future ubiquitous networks because it provides a cheaper alternative to other wired technology currently being used for communication. In smart grid Power Line Communication (PLC) is used to support communication with low rate on low voltage (LV) distribution network. In this paper, we propose the channel modelling of narrowband (NB) PLC in the frequency range 5 KHz to 500 KHz by using ABCD parameter with cyclostationary noise addition. Behaviour of the channel was studied by the addition of 11KV/230V transformer, by varying load location and load. Bit error rate (BER) Vs signal to noise ratio SNR) was plotted for the proposed model by employing OFDM. Our simulation results based on the proposed channel model show an acceptable performance in terms of bit error rate versus signal to noise ratio, which enables communication required for smart grid applications.

SDN-Enabled Dynamic Feedback Control and Sensing in Agile Optical Networks

NASA Astrophysics Data System (ADS)

Lin, Likun

Fiber optic networks are no longer just pipelines for transporting data in the long haul backbone. Exponential growth in traffic in metro-regional areas has pushed higher capacity fiber toward the edge of the network, and highly dynamic patterns of heterogeneous traffic have emerged that are often bursty, severely stressing the historical "fat and dumb pipe" static optical network, which would need to be massively over-provisioned to deal with these loads. What is required is a more intelligent network with a span of control over the optical as well as electrical transport mechanisms which enables handling of service requests in a fast and efficient way that guarantees quality of service (QoS) while optimizing capacity efficiency. An "agile" optical network is a reconfigurable optical network comprised of high speed intelligent control system fed by real-time in situ network sensing. It provides fast response in the control and switching of optical signals in response to changing traffic demands and network conditions. This agile control of optical signals is enabled by pushing switching decisions downward in the network stack to the physical layer. Implementing such agility is challenging due to the response dynamics and interactions of signals in the physical layer. Control schemes must deal with issues such as dynamic power equalization, EDFA transients and cascaded noise effects, impairments due to self-phase modulation and dispersion, and channel-to-channel cross talk. If these issues are not properly predicted and mitigated, attempts at dynamic control can drive the optical network into an unstable state. In order to enable high speed actuation of signal modulators and switches, the network controller must be able to make decisions based on predictive models. In this thesis, we consider how to take advantage of Software Defined Networking (SDN) capabilities for network reconfiguration, combined with embedded models that access updates from deployed network monitoring sensors. In order to maintain signal quality while optimizing network resources, we find that it is essential to model and update estimates of the physical link impairments in real-time. In this thesis, we consider the key elements required to enable an agile optical network, with contributions as follows: • Control Framework: extended the SDN concept to include the optical transport network through extensions to the OpenFlow (OF) protocol. A unified SDN control plane is built to facilitate control and management capability across the electrical/packet-switched and optical/circuit-switched portions of the network seamlessly. The SDN control plane serves as a platform to abstract the resources of multilayer/multivendor networks. Through this platform, applications can dynamically request the network resources to meet their service requirements. • Use of In-situ Monitors: enabled real-time physical impairment sensing in the control plane using in-situ Optical Performance Monitoring (OPM) and bit error rate (BER) analyzers. OPM and BER values are used as quantitative indicators of the link status and are fed to the control plane through a high-speed data collection interface to form a closed-loop feedback system to enable adaptive resource allocation. • Predictive Network Model: used a network model embedded in the control layer to study the link status. The estimated results of network status is fed into the control decisions to precompute the network resources. The performance of the network model can be enhanced by the sensing results. • Real-Time Control Algorithms: investigated various dynamic resource allocation mechanisms supporting an agile optical network. Intelligent routing and wavelength switching for recovering from traffic impairments is achieved experimentally in the agile optical network within one second. A distance-adaptive spectrum allocation scheme to address transmission impairments caused by cascaded Wavelength Selective Switches (WSS) is proposed and evaluated for improving network spectral efficiency.

Human methyl purine DNA glycosylase and DNA polymerase ß expression collectively predict sensitivity to temozolomide

PubMed Central

Trivedi, Ram N.; Wang, Xiao-hong; Jelezcova, Elena; Goellner, Eva M.; Tang, Jiangbo; Sobol, Robert W.

2014-01-01

Over-expression of N-methylpurine DNA glycosylase (MPG) has been suggested as a possible gene therapy approach to sensitize tumor cells to the cell killing effects of temozolomide, an imidazotetrazine-class chemotherapeutic alkylating agent. In the present study, we show that both elevated MPG expression and shRNA-mediated loss of Pol ß expression in human breast cancer cells increases cellular sensitivity to temozolomide. Resistance to temozolomide is restored by complementation of either wild-type human Pol ß or human Pol ß with an inactivating mutation specific to the polymerase active site yet functional for 5′dRP lyase activity. These genetic and cellular studies uniquely demonstrate that over-expression of MPG causes an imbalance in BER leading to an accumulation of cytotoxic 5′dRP lesions and that the 5′dRP lyase activity of Pol ß is required to restore resistance to temozolomide. These results imply that Pol ß dependent 5′dRP lyase activity is the rate-limiting step in BER in these cells and suggests that BER is a tightly balanced pathway for the repair of alkylated bases such as N7-MeG and N3-MeA. Further, we find that 5′dRP-mediated cell death is independent of caspase-3 activation and does not induce the formation of autophagosomes, as measured by GFP-LC3 localization. The experiments presented herein suggest that it will be important to investigate whether an active BER pathway could be partially responsible for the temozolomide-mediated resistance seen in some tumors and that balanced BER protein expression and overall BER capacity may help predict sensitivity to temozolomide. PMID:18477668

Interference-Assisted Techniques for Transmission and Multiple Access in Optical Communications

NASA Astrophysics Data System (ADS)

Guan, Xun

Optical communications can be in wired or wireless form. Fiber optics communication (FOC) connects transmitters and receivers with optical fiber. Benefiting from its high bandwidth, low cost per volume and stability, it gains a significant market share in long-haul networks, access networks and data centers. Meanwhile, optical wireless communication (OWC) is also emerging as a crucial player in the communication market. In OWC, free-space optical communication (FSO) and visible light communication (VLC) are being studied and commercially deployed extensively. Interference is a common phenomenon in multi-user communication systems. In both FOC and OWC, interference has long been treated as a detrimental effect. However, it could also be beneficial to system applications. The effort of harnessing interference has spurred numerous innovations. Interesting examples are physical-layer network coding (PNC) and non-orthogonal multiple access (NOMA). The first part of this thesis in on the topic of PNC. PNC was firstly proposed in wireless communication to improve the throughput of a two-way relay network (TWRN). As a variation of network coding (NC), PNC turns the common channel interference (CCI) as a natural network coding operation. In this thesis, PNC is introduced into optical communication. Three schemes are proposed in different scenarios. Firstly, PNC is applied to a coherent optical orthogonal frequency division multiplexing (CO-OFDM) system so as to improve the throughput of the multicast network. The optical signal to noise ratio (OSNR) penalty is quite low. Secondly, we investigate the application of PNC in an OFDM passive optical network (OFDM-PON) supporting heterogeneous services. It is found that only minor receiver power penalties are observed to realize PNC-based virtual private networks (VPN), both in the wired service part and the wireless service part in an OFDM-PON with heterogeneous services. Thirdly, we innovate relay-based visible light communication (VLC) by adopting PNC, with a newly proposed phase-aligning method. PNC could improve the throughput at the bottlenecking relay node in a VLC system, and the proposed phase aligning method can improve the BER performance. The second part of this thesis discusses another interference-assisted technology in communication, that is, non-orthogonal multiple access (NOMA). NOMA multiplexes signals from multiple users in another dimension: power domain, with a non-orthogonal multiplexing in other dimensions such as time, frequency and code. Three schemes are proposed in this part. The first and the second schemes both realize NOMA in VLC, with different multiuser detection (MUD) techniques and a proposed phase pre-distortion method. Although both can decrease the system BER compared to conventional NOMA, the scheme using joint detection (JD) outperforms the one using successive interference cancellation (SIC). The third scheme investigated in this part is a combination of NOMA and a multicarrier precoding (MP) technology based on an orthogonal circulant transform matrix (OCT). This combination can avoid the complicated adaptive bit loading or electronic equalization, making NOMA more attractive in a practical system.

Flexible high speed CODEC

NASA Technical Reports Server (NTRS)

Wernlund, James V.

1993-01-01

HARRIS, under contract with NASA Lewis, has developed a hard decision BCH (Bose-Chaudhuri-Hocquenghem) triple error correcting block CODEC ASIC, that can be used in either a bursted or continuous mode. the ASIC contains both encoder and decoder functions, programmable lock thresholds, and PSK related functions. The CODEC provides up to 4 dB of coding gain for data rates up to 300 Mbps. The overhead is selectable from 7/8 to 15/16 resulting in minimal band spreading, for a given BER. Many of the internal calculations are brought out enabling the CODEC to be incorporated in more complex designs. The ASIC has been tested in BPSK, QPSK and 16-ary PSK link simulators and found to perform to within 0.1 dB of theory for BER's of 10(exp -2) to 10(exp -9). The ASIC itself, being a hard decision CODEC, is not limited to PSK modulation formats. Unlike most hard decision CODEC's, the HARRIS CODEC doesn't upgrade BER performance significantly at high BER's but rather becomes transparent.

Composite coating of alginate-olive oil enriched with antioxidants enhances postharvest quality and shelf life of Ber fruit (Ziziphus mauritiana Lamk. Var. Gola).

PubMed

Ramana Rao, T V; Baraiya, Nilanjana S; Vyas, Pinal B; Patel, Dhara M

2016-01-01

The present study has been carried out to evaluate the effect of a composite edible coating of 2 % Sodium alginate and 0.2 % Olive oil with combination of 1 % ascorbic acid and 1 % citric acid on the post harvest nutritional quality and shelf life of Ber fruit stored at 25 ± 2 °C and 65 % R.H. The coatings reduced the decay occurrence, weight loss, accumulation of total soluble solids (TSS) and total sugars in Ber fruit and enhanced the level of antioxidants. The delayed activity of polygalacturonase (PG), Pectate lyase (PL) and Pectin methyl esterase (PME) was noticed in coated fruits than that of the control fruit indicating the reduced softening and ripening process. These findings suggest that the composite edible coating tested under the current study has the potential to control decaying incidence of Ber fruit, extends its storage life and also improves its valuable nutritional characteristics.

OFDM-based broadband underwater wireless optical communication system using a compact blue LED

NASA Astrophysics Data System (ADS)

Xu, Jing; Kong, Meiwei; Lin, Aobo; Song, Yuhang; Yu, Xiangyu; Qu, Fengzhong; Han, Jun; Deng, Ning

2016-06-01

We propose and experimentally demonstrate an IM/DD-OFDM-based underwater wireless optical communication system. We investigate the dependence of its BER performance on the training symbol number as well as LED's bias voltage and driving voltage. With single compact blue LED and a low-cost PIN photodiode, we achieve net bit rates of 225.90 Mb/s at a BER of 1.54×10-3 using 16-QAM and 231.95 Mb/s at a BER of 3.28×10-3 using 32-QAM, respectively, over a 2-m air channel. Over a 2-m underwater channel, we achieve net bit rates of 161.36 Mb/s using 16-QAM, 156.31 Mb/s using 32-QAM, and 127.07 Mb/s using 64-QAM, respectively. The corresponding BERs are 2.5×10-3, 7.42×10-4, and 3.17×10-3, respectively, which are all below the FEC threshold.

Was Newtonian cosmology really inconsistent?

NASA Astrophysics Data System (ADS)

Vickers, Peter

This paper follows up a debate as to the consistency of Newtonian cosmology. Whereas Malament [(1995). Is Newtonian cosmology really inconsistent? Philosophy of Science 62, 489-510] has shown that Newtonian cosmology is not inconsistent, to date there has been no analysis of Norton's claim [(1995). The force of Newtonian cosmology: Acceleration is relative. Philosophy of Science 62, 511-522.] that Newtonian cosmology was inconsistent prior to certain advances in the 1930s, and in particular prior to Seeliger's seminal paper of Seeliger [(1895). Über das Newton'sche Gravitationsgesetz. Astronomische Nachrichten 137 (3273), 129-136.] In this paper I agree that there are assumptions, Newtonian and cosmological in character, and relevant to the real history of science, which are inconsistent. But there are some important corrections to make to Norton's account. Here I display for the first time the inconsistencies-four in total-in all their detail. Although this extra detail shows there to be several different inconsistencies, it also goes some way towards explaining why they went unnoticed for 200 years.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guo, Feng; Jin, Tengchuan; Howard, Andrew

The crystallization of the brazil nut allergen Ber e 2 is reported. Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure ismore » currently under way.« less

Poor permeability and absorption affect the activity of four alkaloids from Coptis.

PubMed

Cui, Han-Ming; Zhang, Qiu-Yan; Wang, Jia-Long; Chen, Jian-Long; Zhang, Yu-Ling; Tong, Xiao-Lin

2015-11-01

Coptidis rhizoma (Coptis) and its alkaloids exert various pharmacological functions in cells and tissues; however, the oral absorption of these alkaloids requires further elucidation. The present study aimed to examine the mechanism underlying the poor absorption of alkaloids, including berberine (BER), coptisine (COP), palmatine (PAL) and jatrorrhizine (JAT). An ultra‑performance liquid chromatography (UPLC) method was validated for the determination of BER, COP, PAL and JAT in the above experimental medium. In addition, the apparent oil‑water partition coefficient (Po/w); apparent permeability coefficient (Papp), determined using a parallel artificial membrane permeability assay (PAMPA) plate; membrane retention coefficient (R %); and effect of P‑glycoprotein (P‑gp) inhibitor on the Papp of the four alkaloids were investigated. The intestinal absorption rate constant (Ka) and absorption percentage (A %) of the four alkaloids were also determined. The results of the present study demonstrated that the Po/w of the four alkaloids in 0.1 mol·l‑1 HCl medium was significantly higher (P<0.01), compared with those of the alkaloids in phosphate buffer (pH 7.4). The Papp of BER was 1.0‑1.2x10‑6 cm·s‑1, determined using a PAMPA plate, and the Papp of BER, COP, PAL and JAT decreased sequentially. The concentrations of the four alkaloids on the apical‑to‑basolateral (AP‑BL) surface and the basolateral‑to‑apical (BL‑AP) surface increased in a linear manner, with increasing concentrations between 10 and 100 µmol. In addition, the transportation of BER on the BL‑AP surface was significantly faster (P<0.01), compared with that on the AP‑BL surface and, following the addition of verpamil (a P‑gp inhibitor), the Papp (AP‑BL) of the four alkaloids increased, whereas the Papp (BL‑AP) was significantly decreased (P<0.01). The rat intestinal perfusion experiment demonstrated that the four alkaloids were poorly absorbed; however, the Ka of BER was significantly higher, compared with the three other alkaloids. Furthermore, the A % and Ka provided evidence that the absorption of BER was increased in the jejunum, compared with in the ileum. In conclusion, the four alkaloids from Coptis appeared to be poorly absorbed, determined using a shake flask, pre‑coated PAMPA plates, a Caco‑2 cell monolayer model and intestinal perfusion; however, absorption was higher in the jejunum than in the ileum. Among the four alkaloids, the permeability of BER was markedly higher than the others, and P‑gp efflux had a significant effect on the absorption of those alkaloids.

Evaluation of the fatigue and toughness of fiber reinforced concrete for use as a new highway pavement design : tech summary.

DOT National Transportation Integrated Search

2016-04-01

This study presents the fi rst approach to develop a new concrete pavement structure reinforced only with fi bers. This : research will identify probable combinations of fi bers (dosage and length combinations) that will adequately perform : in repea...

Network biology: Describing biological systems by complex networks. Comment on "Network science of biological systems at different scales: A review" by M. Gosak et al.

NASA Astrophysics Data System (ADS)

Jalili, Mahdi

2018-03-01

I enjoyed reading Gosak et al. review on analysing biological systems from network science perspective [1]. Network science, first started within Physics community, is now a mature multidisciplinary field of science with many applications ranging from Ecology to biology, medicine, social sciences, engineering and computer science. Gosak et al. discussed how biological systems can be modelled and described by complex network theory which is an important application of network science. Although there has been considerable progress in network biology over the past two decades, this is just the beginning and network science has a great deal to offer to biology and medical sciences.

Combined peak-to-average power ratio reduction and physical layer security enhancement in optical orthogonal frequency division multiplexing visible-light communication systems

NASA Astrophysics Data System (ADS)

Wang, Zhongpeng; Chen, Shoufa

2016-07-01

A physical encryption scheme for discrete Hartley transform (DHT) precoded orthogonal frequency division multiplexing (OFDM) visible-light communication (VLC) systems using frequency domain chaos scrambling is proposed. In the scheme, the chaos scrambling, which is generated by a modified logistic mapping, is utilized to enhance the physical layer of security, and the DHT precoding is employed to reduce of OFDM signal for OFDM-based VLC. The influence of chaos scrambling on peak-to-average power ratio (PAPR) and bit error rate (BER) of systems is studied. The experimental simulation results prove the efficiency of the proposed encryption method for DHT-precoded, OFDM-based VLC systems. Furthermore, the influence of the proposed encryption to the PAPR and BER of systems is evaluated. The experimental results show that the proposed security scheme can protect the DHT-precoded, OFDM-based VLC from eavesdroppers, while keeping the good BER performance of DHT-precoded systems. The BER performance of the encrypted and DHT-precoded system is almost the same as that of the conventional DHT-precoded system without encryption.

The effect of feeding native warm season grasses in the stocker phase on the carcass quality, meat quality, and sensory attributes of beef loin steaks from grain-finished steers.

PubMed

Kurve, V P; Joseph, P; Williams, J B; Kim, T J; Boland, H; Smith, T; Schilling, M W

2016-02-01

The objective of this study was to evaluate the effect of feeding native warm season grass (NWSG) to beef cattle during the stocker phase (110days) on carcass and meat quality. Treatments included Bermudagrass (BER), Indiangrass monoculture (IND), and a mixture of NWSG including Big Bluestem, Little Bluestem, and Indiangrass (MIX). Cattle were finished on grain (180days) in a commercial feedlot and carcass data were collected after harvest. The percentages of 'choice' carcasses were 100, 95.8, and 87 within MIX, BER, and IND treatments, respectively. Steaks from BER had greater (P<0.05) fat content, and lower (P<0.05) protein and moisture percentages when compared to steaks from IND and MIX treatments. However, steaks from the MIX and IND treatments had less (P<0.05) lipid oxidation than steaks from BER after 6days of refrigerated storage. Results indicate that NWSG could be effectively incorporated into forage systems for stocker cattle without compromising carcass and meat quality. Copyright © 2015 Elsevier Ltd. All rights reserved.

Adenomatous Polyposis Coli Interacts with Flap Endonuclease 1 to Block Its Nuclear Entry and Function1

PubMed Central

Jaiswal, Aruna S; Armas, Melissa L; Izumi, Tadahide; Strauss, Phyllis R; Narayan, Satya

2012-01-01

In previous studies, we found that adenomatous polyposis coli (APC) blocks the base excision repair (BER) pathway by interacting with 5′-flap endonuclease 1 (Fen1). In this study, we identify the molecular features that contribute to the formation and/or stabilization of the APC/Fen1 complex that determines the extent of BER inhibition, and the subsequent accumulation of DNA damage creates mutagenic lesions leading to transformation susceptibility. We show here that APC binds to the nuclear localization sequence of Fen1 (Lys365Lys366Lys367), which prevents entry of Fen1 into the nucleus and participation in Pol-β-directed long-patch BER. We also show that levels of the APC/Fen1 complex are higher in breast tumors than in the surrounding normal tissues. These studies demonstrate a novel role for APC in the suppression of Fen1 activity in the BER pathway and a new biomarker profile to be explored to identify individuals who may be susceptible to the development of mammary and other tumors. PMID:22787431

Analytical estimation of laser phase noise induced BER floor in coherent receiver with digital signal processing.

PubMed

Vanin, Evgeny; Jacobsen, Gunnar

2010-03-01

The Bit-Error-Ratio (BER) floor caused by the laser phase noise in the optical fiber communication system with differential quadrature phase shift keying (DQPSK) and coherent detection followed by digital signal processing (DSP) is analytically evaluated. An in-phase and quadrature (I&Q) receiver with a carrier phase recovery using DSP is considered. The carrier phase recovery is based on a phase estimation of a finite sum (block) of the signal samples raised to the power of four and the phase unwrapping at transitions between blocks. It is demonstrated that errors generated at block transitions cause the dominating contribution to the system BER floor when the impact of the additive noise is negligibly small in comparison with the effect of the laser phase noise. Even the BER floor in the case when the phase unwrapping is omitted is analytically derived and applied to emphasize the crucial importance of this signal processing operation. The analytical results are verified by full Monte Carlo simulations. The BER for another type of DQPSK receiver operation, which is based on differential phase detection, is also obtained in the analytical form using the principle of conditional probability. The principle of conditional probability is justified in the case of differential phase detection due to statistical independency of the laser phase noise induced signal phase error and the additive noise contributions. Based on the achieved analytical results the laser linewidth tolerance is calculated for different system cases.

Cost-effective TCM-based WDM-PON for highly asymmetric traffic conditions.

PubMed

Lee, Danbi; Kwon, Won-Bae; Chae, Chang-Joon; Park, Chang-Soo

2015-11-16

A time compression multiplexing (TCM)-based wavelength division multiplexing passive optical network (WDM-PON) using a reflective semiconductor optical amplifier (RSOA) is proposed, and its feasibility is experimentally demonstrated. In the proposed system, the RSOA pre-amplifies a 10 Gb/s downstream signal and modulates the RSOA output, wavelength-locked to the downstream signal, with a 1.25 Gb/s upstream signal simultaneously. The sensitivity of the downstream signal is improved by about 3 dB through the RSOA. The downstream and upstream signals have power penalties of about 0.1 dB and 1.1 dB, respectively, at bit error rates (BERs) of 10(-9) after 20 km transmission.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mohapatra, Purusottam; Satapathy, Shakti Ranjan; Das, Dipon

Cigarette smoking is a key factor for the development and progression of different cancers including mammary tumor in women. Resveratrol (Res) is a promising natural chemotherapeutic agent that regulates many cellular targets including p21, a cip/kip family of cyclin kinase inhibitors involved in DNA damage-induced cell cycle arrest and blocking of DNA replication and repair. We have recently shown that cigarette smoke condensate (CSC) prepared from commercially available Indian cigarette can cause neoplastic transformation of normal breast epithelial MCF-10A cell. Here we studied the mechanism of Res mediated apoptosis in CSC transformed (MCF-10A-Tr) cells in vitro and in vivo. Resmore » mediated apoptosis in MCF-10A-Tr cells was a p21 dependent event. It increased the p21 protein expression in MCF-10A-Tr cells and MCF-10A-Tr cells-mediated tumors in xenograft mice. Res treatment reduced the tumor size(s) and expression of anti-apoptotic proteins (e.g. PI3K, AKT, NFκB) in solid tumor. The expressions of cell cycle regulatory (Cyclins, CDC-2, CDC-6, etc.), BER associated (Pol-β, Pol-δ, Pol-ε, Pol-η, RPA, Fen-1, DNA-Ligase-I, etc.) proteins and LP-BER activity decreased in MCF-10A-Tr cells but remain significantly unaltered in isogenic p21 null MCF-10A-Tr cells after Res treatment. Interestingly, no significant changes were noted in SP-BER activity in both the cell lines after Res exposure. Finally, it was observed that increased p21 blocks the LP-BER in MCF-10A-Tr cells by increasing its interaction with PCNA via competing with Fen-1 after Res treatment. Thus, Res caused apoptosis in CSC-induced cancer cells by reduction of LP-BER activity and this phenomenon largely depends on p21. - Highlights: • Resveratrol (Res) caused reduction of MCF-10A-Tr cell growth by inducing apoptosis. • Res caused cell cycle arrest and DNA damage in p21 dependent manner. • Res mediated LP-BER reduction in MCF-10A-Tr cells was a p21 dependent phenomenon. • Res inhibits BER and PI3K, AKT, and NFκB protein expressions in tumor and xenografts. • Res-induced-p21 inhibited DNA repair by modulating Fen-1 binding to PCNA complex.« less

Evaluation of the RPi-ber late blight resistance gene for tuber resistance in the field and laboratory.

USDA-ARS?s Scientific Manuscript database

The purpose of this research was to evaluate the efficacy in tubers of a late blight resistance gene, RPi-ber, originating from Solanum berthaultii. Experiments were conducted in the field and laboratory. Inoculation of tubers in field trials occurred via sporangia produced on infections in the foli...

Behavioral and Emotional Strength-Based Assessment of Finnish Elementary Students: Psychometrics of the BERS-2

ERIC Educational Resources Information Center

Sointu, Erkko Tapio; Savolainen, Hannu; Lambert, Matthew C.; Lappalainen, Kristiina; Epstein, Michael H.

2014-01-01

When rating scales are used in different countries, thorough investigation of the psychometric properties is needed. We examined the internal structure of the Finnish translated Behavioral and Emotional Rating Scale-2 (BERS-2) using Rasch and confirmatory factor analysis approaches with a sample of youth, parents, and teachers. The results…

Augmented Reality-Based Simulators as Discovery Learning Tools: An Empirical Study

ERIC Educational Resources Information Center

Ibáñez, María-Blanca; Di-Serio, Ángela; Villarán-Molina, Diego; Delgado-Kloos, Carlos

2015-01-01

This paper reports empirical evidence on having students use AR-SaBEr, a simulation tool based on augmented reality (AR), to discover the basic principles of electricity through a series of experiments. AR-SaBEr was enhanced with knowledge-based support and inquiry-based scaffolding mechanisms, which proved useful for discovery learning in…

Über den Beitrag von Heinrich Bruns zur theoretischen geometrischen Optik - unter Berücksichtigung seines Briefwechsels mit Wissenschaftlern der Zeiss-Werke in Jena 1888 - 1893.

NASA Astrophysics Data System (ADS)

Ilgauds, H.-J.; Münzel, G.

Heinrich Bruns, director of the Leipzig University Observatory, was working on theoretical geometrical optics, and applied this to practical questions. His correspondence with opticians of the Zeiss Company in Jena gives evidence of their mutual regard and inspiration.

MPI investigation for 40G NRZ link with low-RL cable assemblies

NASA Astrophysics Data System (ADS)

Satake, Toshiaki; Berdinskikh, Tatiana; Thongdaeng, Rutsuda; Faysanyo, Pitak; Gurreri, Michael

2017-01-01

Bit Error Ratio (BER) dependence on received power was studied for 40Gb/s NRZ short optical fiber transmission, including a series of four low return loss (RL 21dB) and low insertion loss (IL 0.1dB) connections. The calculated power penalty (PP) was 0.15dB for BER 10-11. Although the fiber length was within DFB laser's coherent length of 100m and the multi path interference (MPI) value was 34.3dB, no PP of BER was observed. There was no PP due to low MPI probably because the polarization of the signal pulses were not aligned for optical interference, indicating that NRZ systems have a high resistance to MPI.

Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes.

PubMed

Maher, R L; Marsden, C G; Averill, A M; Wallace, S S; Sweasy, J B; Pederson, D S

2017-09-01

Reactive oxygen species generate some 20,000 base lesions per human cell per day. The vast majority of these potentially mutagenic or cytotoxic lesions are subject to base excision repair (BER). Although chromatin remodelers have been shown to enhance the excision of oxidized bases from nucleosomes in vitro, it is not clear that they are recruited to and act at sites of BER in vivo. To test the hypothesis that cells possess factors that enhance BER in chromatin, we assessed the capacity of nuclear extracts from human cells to excise thymine glycol (Tg) lesions from exogenously added, model nucleosomes. The DNA glycosylase NTHL1 in these extracts was able to excise Tg from both naked DNA and sites in nucleosomes that earlier studies had shown to be sterically accessible. However, the same extracts were able to excise lesions from sterically-occluded sites in nucleosomes only after the addition of Mg 2+ /ATP. Gel mobility shift assays indicated that nucleosomes remain largely intact following the Mg 2+ /ATP -dependent excision reaction. Size exclusion chromatography indicated that the NTHL1-stimulating activity has a relatively low molecular weight, close to that of NTHL1 and other BER glycosylases; column fractions that contained the very large chromatin remodeling complexes did not exhibit this same stimulatory activity. These results indicate that cells possess a factor(s) that promotes the initiation of BER in chromatin, but differs from most known chromatin remodeling complexes. Copyright © 2017 Elsevier B.V. All rights reserved.

Analytical evaluation of the combined influence of polarization mode dispersion and group velocity dispersion on the bit error rate performance of optical homodyne quadrature phase-shift keying systems

NASA Astrophysics Data System (ADS)

Taher, Kazi Abu; Majumder, Satya Prasad

2017-12-01

A theoretical approach is presented to evaluate the bit error rate (BER) performance of an optical fiber transmission system with quadrature phase-shift keying (QPSK) modulation under the combined influence of polarization mode dispersion (PMD) and group velocity dispersion (GVD) in a single-mode fiber (SMF). The analysis is carried out without and with polarization division multiplexed (PDM) transmission considering a coherent homodyne receiver. The probability density function (pdf) of the random phase fluctuations due to PMD and GVD at the output of the receiver is determined analytically, considering the pdf of differential group delay (DGD) to be Maxwellian distribution and that of GVD to be Gaussian approximation. The exact pdf of the phase fluctuation due to PMD and GVD is also evaluated from its moments using a Monte Carlo simulation technique. Average BER is evaluated by averaging the conditional BER over the pdf of the random phase fluctuation. The BER performance results are evaluated for different system parameters. It is found that PDM-QPSK coherent homodyne system suffers more power penalty than the homodyne QPSK system without PDM. A PDM-QPSK system suffers a penalty of 4.3 dB whereas power penalty of QPSK system is 3.0 dB at a BER of 10-9 for DGD of 0.8 Tb and GVD of 1700 ps/nm. Analytical results are compared with the experimental results reported earlier and found to have good conformity.

Performance analysis of LDPC codes on OOK terahertz wireless channels

NASA Astrophysics Data System (ADS)

Chun, Liu; Chang, Wang; Jun-Cheng, Cao

2016-02-01

Atmospheric absorption, scattering, and scintillation are the major causes to deteriorate the transmission quality of terahertz (THz) wireless communications. An error control coding scheme based on low density parity check (LDPC) codes with soft decision decoding algorithm is proposed to improve the bit-error-rate (BER) performance of an on-off keying (OOK) modulated THz signal through atmospheric channel. The THz wave propagation characteristics and channel model in atmosphere is set up. Numerical simulations validate the great performance of LDPC codes against the atmospheric fading and demonstrate the huge potential in future ultra-high speed beyond Gbps THz communications. Project supported by the National Key Basic Research Program of China (Grant No. 2014CB339803), the National High Technology Research and Development Program of China (Grant No. 2011AA010205), the National Natural Science Foundation of China (Grant Nos. 61131006, 61321492, and 61204135), the Major National Development Project of Scientific Instrument and Equipment (Grant No. 2011YQ150021), the National Science and Technology Major Project (Grant No. 2011ZX02707), the International Collaboration and Innovation Program on High Mobility Materials Engineering of the Chinese Academy of Sciences, and the Shanghai Municipal Commission of Science and Technology (Grant No. 14530711300).

Demonstration of 20Gb/s polarization-insensitive wavelength switching system for high-speed free-space optical network

NASA Astrophysics Data System (ADS)

Qian, Feng-chen; Ye, Ya-lin; Wen, Yu; Duan, Tao; Feng, Huan

2015-10-01

A 20Gb/s polarization-insensitive all-optical wavelength switching system for high-speed free-space optical communication (FSO) network is experimentally demonstrated All-optical wavelength conversion (AOWC) is implemented using four-wave mixing (FWM) by highly-nonlinear fiber (HNLF). In the experimental setup, a simple actively mode-locked fiber ring laser (AML-FRL) with repetition frequency from 1 to 15 GHz is used to generate eight 2.5Gb/s tributary signals, which are multiplexed into one 20Gb/s optical data stream. At the receiver, the 20 Gb/s OTDM data stream is demultiplexed down to 2.5 Gb/s via a polarization-insensitive FWM scheme. The whole space communication distance is over 10 meters in building hallway. The experimental results show that this system can stably run over 24 hours at 10-9 BER level, thus the proposed architecture can work at higher rate with wavelength-division multiplexing (WDM) and high order modulation schemes.

Simultaneous generation of 40, 80 and 120 GHz optical millimeter-wave from one Mach-Zehnder modulator and demonstration of millimeter-wave transmission and down-conversion

NASA Astrophysics Data System (ADS)

Zhou, Wen; Qin, Chaoyi

2017-09-01

We demonstrate multi-frequency QPSK millimeter-wave (mm-wave) vector signal generation enabled by MZM-based optical carrier suppression (OCS) modulation and in-phase/quadrature (I/Q) modulation. We numerically simulate the generation of 40-, 80- and 120-GHz vector signal. Here, the three different signals carry the same QPSK modulation information. We also experimentally realize 11Gbaud/s QPSK vector signal transmission over 20 km fiber, and the generation of the vector signals at 40-GHz, 80-GHz and 120-GHz. The experimental results show that the bit-error-rate (BER) for all the three different signals can reach the forward-error-correction (FEC) threshold of 3.8×10-3. The advantage of the proposed system is that provide high-speed, high-bandwidth and high-capacity seamless access of TDM and wireless network. These features indicate the important application prospect in wireless access networks for WiMax, Wi-Fi and 5G/LTE.

Convergent Validity with the BERS-2 Teacher Rating Scale and the Achenbach Teacher's Report Form: A Replication and Extension

ERIC Educational Resources Information Center

Benner, Gregory J.; Beaudoin, Kathleen; Mooney, Paul; Uhing, Brad M.; Pierce, Corey D.

2008-01-01

In the present study, we sought to extend instrument validation research for a strength-based emotional and behavior rating scale, the "Teacher Rating Scale of the Behavior and Emotional Rating Scale-Second Edition" (BERS-2; Epstein, M. H. (2004). "Behavioral and emotional rating scale" (2nd ed.). Austin, TX: PRO-ED) through…

How Photography as Field Notes Helps in Understanding the Building the Education Revolution

ERIC Educational Resources Information Center

Loughlin, Jill

2013-01-01

This paper is the outcome of research conducted between June 2010 and January 2011 as part of understanding the building the education revolution (BER) a major policy initiative of the Australian Federal Government which commenced when Julia Gillard, now PM, was the Federal Minister of Education. The BER policy initiative was part of an economic…

Assay of phenolic compounds from four species of Ber (Ziziphus mauritiana L.) Fruits: Comparision of three base hydrolysis procedure for quantification of total phenolic acids

USDA-ARS?s Scientific Manuscript database

The present study was undertaken to investigate the flavonoids profile in four species of ber (Ziziphus mauritiana Lamk) fruit and to compare various techniques for the analysis of total phenolic acids. The 12 flavonoids identified were quercetin 3-O-robinobioside, quercetin 3-O-rutinoside, querceti...

Zeit im Wandel der Zeit.

NASA Astrophysics Data System (ADS)

Aichelburg, P. C.

Contents: Einleitung(P. C. Aichelburg). 1. Über Zeit, Bewegung und Veränderung (Aristoteles). 2. Ewigkeit und Zeit (Plotin). 3. Was ist die Zeit? (Augustinus). 4. Von der Zeit (Immanuel Kant). 5. Newtons Ansichten über Zeit, Raum und Bewegung (Ernst Mach). 6. Über die mechanische Erklärung irreversibler Vorgänge (Ludwig Boltzmann). 7. Das Maß der Zeit (Henri Poincaré). 8. Dauer und Intuition (Henri Bergson). 9. Die Geschichte des Unendlichkeitsproblems (Bertrand Russell). 10. Raum und Zeit (Hermann Minkowski). 11. Der Unterschied von Zeit und Raum (Hans Reichenbach). 12. Newtonscher und Bergsonscher Zeitbegriff (Norbert Wiener). 13. Die Bildung des Zeitbegriffs beim Kinde (JeanPiaget).14. Eine Bemerkung über die Beziehungen zwischen Relativitätstheorie und der idealistischen Philosophie (Kurt Gödel). 15. Der zweite Hauptsatz und der Unterschied von Vergangenheit und Zukunft (Carl Friedrich v. Weizsäcker). 16. Zeit als physikalischer Begriff (Friedrich Hund). 17. Zeitmessung und Zeitbegriff in der Astronomie (Otto Heckmann). 18. Kann die Zeit rückwärts gehen? (Martin Gardner). 19. Zeit und Zeiten (Ilya Prigogine, Isabelle Stengers). 20. Zeit als dynamische Größe in der Relativitätstheorie (P. C. Aichelburg).

Performance of MIMO-OFDM using convolution codes with QAM modulation

NASA Astrophysics Data System (ADS)

Astawa, I. Gede Puja; Moegiharto, Yoedy; Zainudin, Ahmad; Salim, Imam Dui Agus; Anggraeni, Nur Annisa

2014-04-01

Performance of Orthogonal Frequency Division Multiplexing (OFDM) system can be improved by adding channel coding (error correction code) to detect and correct errors that occur during data transmission. One can use the convolution code. This paper present performance of OFDM using Space Time Block Codes (STBC) diversity technique use QAM modulation with code rate ½. The evaluation is done by analyzing the value of Bit Error Rate (BER) vs Energy per Bit to Noise Power Spectral Density Ratio (Eb/No). This scheme is conducted 256 subcarrier which transmits Rayleigh multipath fading channel in OFDM system. To achieve a BER of 10-3 is required 10dB SNR in SISO-OFDM scheme. For 2×2 MIMO-OFDM scheme requires 10 dB to achieve a BER of 10-3. For 4×4 MIMO-OFDM scheme requires 5 dB while adding convolution in a 4x4 MIMO-OFDM can improve performance up to 0 dB to achieve the same BER. This proves the existence of saving power by 3 dB of 4×4 MIMO-OFDM system without coding, power saving 7 dB of 2×2 MIMO-OFDM and significant power savings from SISO-OFDM system.

Aag DNA Glycosylase Promotes Alkylation-Induced Tissue Damage Mediated by Parp1

PubMed Central

Calvo, Jennifer A.; Moroski-Erkul, Catherine A.; Lake, Annabelle; Eichinger, Lindsey W.; Shah, Dharini; Jhun, Iny; Limsirichai, Prajit; Bronson, Roderick T.; Christiani, David C.; Meira, Lisiane B.; Samson, Leona D.

2013-01-01

Alkylating agents comprise a major class of front-line cancer chemotherapeutic compounds, and while these agents effectively kill tumor cells, they also damage healthy tissues. Although base excision repair (BER) is essential in repairing DNA alkylation damage, under certain conditions, initiation of BER can be detrimental. Here we illustrate that the alkyladenine DNA glycosylase (AAG) mediates alkylation-induced tissue damage and whole-animal lethality following exposure to alkylating agents. Aag-dependent tissue damage, as observed in cerebellar granule cells, splenocytes, thymocytes, bone marrow cells, pancreatic β-cells, and retinal photoreceptor cells, was detected in wild-type mice, exacerbated in Aag transgenic mice, and completely suppressed in Aag −/− mice. Additional genetic experiments dissected the effects of modulating both BER and Parp1 on alkylation sensitivity in mice and determined that Aag acts upstream of Parp1 in alkylation-induced tissue damage; in fact, cytotoxicity in WT and Aag transgenic mice was abrogated in the absence of Parp1. These results provide in vivo evidence that Aag-initiated BER may play a critical role in determining the side-effects of alkylating agent chemotherapies and that Parp1 plays a crucial role in Aag-mediated tissue damage. PMID:23593019

Multiple-access relaying with network coding: iterative network/channel decoding with imperfect CSI

NASA Astrophysics Data System (ADS)

Vu, Xuan-Thang; Renzo, Marco Di; Duhamel, Pierre

2013-12-01

In this paper, we study the performance of the four-node multiple-access relay channel with binary Network Coding (NC) in various Rayleigh fading scenarios. In particular, two relay protocols, decode-and-forward (DF) and demodulate-and-forward (DMF) are considered. In the first case, channel decoding is performed at the relay before NC and forwarding. In the second case, only demodulation is performed at the relay. The contributions of the paper are as follows: (1) two joint network/channel decoding (JNCD) algorithms, which take into account possible decoding error at the relay, are developed in both DF and DMF relay protocols; (2) both perfect channel state information (CSI) and imperfect CSI at receivers are studied. In addition, we propose a practical method to forward the relays error characterization to the destination (quantization of the BER). This results in a fully practical scheme. (3) We show by simulation that the number of pilot symbols only affects the coding gain but not the diversity order, and that quantization accuracy affects both coding gain and diversity order. Moreover, when compared with the recent results using DMF protocol, our proposed DF protocol algorithm shows an improvement of 4 dB in fully interleaved Rayleigh fading channels and 0.7 dB in block Rayleigh fading channels.

A Simplified GCS-DCSK Modulation and Its Performance Optimization

NASA Astrophysics Data System (ADS)

Xu, Weikai; Wang, Lin; Chi, Chong-Yung

2016-12-01

In this paper, a simplified Generalized Code-Shifted Differential Chaos Shift Keying (GCS-DCSK) whose transmitter never needs any delay circuits, is proposed. However, its performance is deteriorated because the orthogonality between substreams cannot be guaranteed. In order to optimize its performance, the system model of the proposed GCS-DCSK with power allocations on substreams is presented. An approximate bit error rate (BER) expression of the proposed model, which is a function of substreams’ power, is derived using Gaussian Approximation. Based on the BER expression, an optimal power allocation strategy between information substreams and reference substream is obtained. Simulation results show that the BER performance of the proposed GCS-DCSK with the optimal power allocation can be significantly improved when the number of substreams M is large.

Linewidth-tolerant 10-Gbit/s 16-QAM transmission using a pilot-carrier based phase-noise cancelling technique.

PubMed

Nakamura, Moriya; Kamio, Yukiyoshi; Miyazaki, Tetsuya

2008-07-07

We experimentally demonstrated linewidth-tolerant 10-Gbit/s (2.5-Gsymbol/s) 16-quadrature amplitude modulation (QAM) by using a distributed-feedback laser diode (DFB-LD) with a linewidth of 30 MHz. Error-free operation, a bit-error rate (BER) of <10(-9) was achieved in transmission over 120 km of standard single mode fiber (SSMF) without any dispersion compensation. The phase-noise canceling capability provided by a pilot-carrier and standard electronic pre-equalization to suppress inter-symbol interference (ISI) gave clear 16-QAM constellations and floor-less BER characteristics. We evaluated the BER characteristics by real-time measurement of six (three different thresholds for each I- and Q-component) symbol error rates (SERs) with simultaneous constellation observation.

A Passion for Discovery

ScienceCinema

Freund, Peter

2017-12-11

The human side of doing theoretical physics is explored through stories about the interactions between physicists and about the way world events can affect not only the scientists' behavior, but even their scientific interests and style.  These stories cluster nicely around certain bigger themes to create an overarching whole.  This happens both on account of some interesting narrative structures intrinsic to the science of Physics itself and on account of the way Physics integrates into the general culture. The stories concern Einstein, Schrödinger, Pauli, Heisenberg, Stueckelberg, Jordan and Fock and also involve some mathematicians like Emmy Noether, Teichmüller and Bers and even the psychologist C.G. Jung.

Behavioral and hormonal responses to stress in binge-like eating prone female rats.

PubMed

Calvez, Juliane; Timofeeva, Elena

2016-04-01

Binge eating episodes are frequently stimulated by stress. We developed a model of binge eating proneness based on individual sensitivity of young female Sprague Dawley rats to significantly increase sucrose consumption in response to stress. The rats were subjected to unpredictable intermittent 1-h access to 10% sucrose. After the stabilization of sucrose intake, rats were assessed for consistency of higher (for binge-like eating prone, BEP) or lower (for binge-like eating resistant, BER) sucrose intake in response to unpredictable episodes of foot-shock stress. The objectives of this study included demonstrating face validity of the BEP model and determining if some of the features of this model were pre-existing before exposure to intermittent access to sucrose and repeated stress. The BEP rats consumed a larger (20%>BER) amount of sucrose in a discrete (1-h) period of time compared to the BER phenotype in non-stressful conditions and significantly increased sucrose intake (50%>BER) under stress. Conversely, stress did not affect sucrose intake in BER rats. BEP rats showed higher sucrose intake compared to BER rats at the beginning of darkness as well as during the light period when they were sated and not physically hungry. Analyses of the sucrose licking microstructure revealed that BEP rats had a high motivational drive to consume sucrose in non-stressful condition and an increased hedonic value of sucrose when they were exposed to stressful conditions. BEP rats consumed sucrose much more rapidly under stressful conditions compared to BER rats. Finally, BEP rats demonstrated compulsive-like intake of sucrose (assessed in the light-dark box) and a blunted stress-induced increase in plasma corticosterone levels. Body weight and chow intake were not different between the phenotypes. Before exposure to intermittent access to sucrose and repeated stress, the BEP rats showed no clear evidence for compulsive sucrose intake. However, from the first 1-h access to sucrose, the BEP rats exhibited sucrose overeating; and from the first exposure to stress before intermittent access to sucrose, the BEP rats showed a blunted increase in corticosterone plasma levels. Innate sucrose hyperconsumption and altered reactivity of the hypothalamo-pituitary adrenal (HPA) axis to stress may be involved in the development of binge-like eating. Increased perceived hedonic value of palatable food and an increased motivation to consume this food despite aversive conditions as well as deregulated reactivity of the HPA axis may contribute to stress-induced bingeing on sucrose in BEP rats. Copyright © 2016 Elsevier Inc. All rights reserved.

Rational Inhibitors of DNA Base Excision Repair (BER) Enzymes: New Tools for Elucidating the Role of the BER in Cancer Chemotherapy

DTIC Science & Technology

2005-05-01

Revised Manuscript Received March 3, 2005 ABSTRACT: Base flipping is a highly conserved strategy used by enzymes to gain catalytic access to DNA bases that...73.13. Jencks, W. P. (1985) A primer for the bema hapothle-an between uracil and other normal DNA bases at this inter- empirical-approach to the

Strategies for P2P connectivity in reconfigurable converged wired/wireless access networks.

PubMed

Puerto, Gustavo; Mora, José; Ortega, Beatriz; Capmany, José

2010-12-06

This paper presents different strategies to define the architecture of a Radio-Over-Fiber (RoF) Access networks enabling Peer-to-Peer (P2P) functionalities. The architectures fully exploit the flexibility of a wavelength router based on the feedback configuration of an Arrayed Waveguide Grating (AWG) and an optical switch to broadcast P2P services among diverse infrastructures featuring dynamic channel allocation and enabling an optical platform for 3G and beyond wireless backhaul requirements. The first architecture incorporates a tunable laser to generate a dedicated wavelength for P2P purposes and the second architecture takes advantage of reused wavelengths to enable the P2P connectivity among Optical Network Units (ONUs) or Base Stations (BS). While these two approaches allow the P2P connectivity in a one at a time basis (1:1), the third architecture enables the broadcasting of P2P sessions among different ONUs or BSs at the same time (1:M). Experimental assessment of the proposed architecture shows approximately 0.6% Error Vector Magnitude (EVM) degradation for wireless services and 1 dB penalty in average for 1 x 10(-12) Bit Error Rate (BER) for wired baseband services.

Dispersion compensation of fiber optic communication system with direct detection using artificial neural networks (ANNs)

NASA Astrophysics Data System (ADS)

Maghrabi, Mahmoud M. T.; Kumar, Shiva; Bakr, Mohamed H.

2018-02-01

This work introduces a powerful digital nonlinear feed-forward equalizer (NFFE), exploiting multilayer artificial neural network (ANN). It mitigates impairments of optical communication systems arising due to the nonlinearity introduced by direct photo-detection. In a direct detection system, the detection process is nonlinear due to the fact that the photo-current is proportional to the absolute square of the electric field intensity. The proposed equalizer provides the most efficient computational cost with high equalization performance. Its performance is comparable to the benchmark compensation performance achieved by maximum-likelihood sequence estimator. The equalizer trains an ANN to act as a nonlinear filter whose impulse response removes the intersymbol interference (ISI) distortions of the optical channel. Owing to the proposed extensive training of the equalizer, it achieves the ultimate performance limit of any feed-forward equalizer (FFE). The performance and efficiency of the equalizer is investigated by applying it to various practical short-reach fiber optic communication system scenarios. These scenarios are extracted from practical metro/media access networks and data center applications. The obtained results show that the ANN-NFFE compensates for the received BER degradation and significantly increases the tolerance to the chromatic dispersion distortion.

Performance Analysis of OCDMA Based on AND Detection in FTTH Access Network Using PIN & APD Photodiodes

NASA Astrophysics Data System (ADS)

Aldouri, Muthana; Aljunid, S. A.; Ahmad, R. Badlishah; Fadhil, Hilal A.

2011-06-01

In order to comprise between PIN photo detector and avalanche photodiodes in a system used double weight (DW) code to be a performance of the optical spectrum CDMA in FTTH network with point-to-multi-point (P2MP) application. The performance of PIN against APD is compared through simulation by using opt system software version 7. In this paper we used two networks designed as follows one used PIN photo detector and the second using APD photo diode, both two system using with and without erbium doped fiber amplifier (EDFA). It is found that APD photo diode in this system is better than PIN photo detector for all simulation results. The conversion used a Mach-Zehnder interferometer (MZI) wavelength converter. Also we are study, the proposing a detection scheme known as AND subtraction detection technique implemented with fiber Bragg Grating (FBG) act as encoder and decoder. This FBG is used to encode and decode the spectral amplitude coding namely double weight (DW) code in Optical Code Division Multiple Access (OCDMA). The performances are characterized through bit error rate (BER) and bit rate (BR) also the received power at various bit rate.

2x2 MIMO-OFDM Gigabit fiber-wireless access system based on polarization division multiplexed WDM-PON.

PubMed

Deng, Lei; Pang, Xiaodan; Zhao, Ying; Othman, M B; Jensen, Jesper Bevensee; Zibar, Darko; Yu, Xianbin; Liu, Deming; Monroy, Idelfonso Tafur

2012-02-13

We propose a spectral efficient radio over wavelength division multiplexed passive optical network (WDM-PON) system by combining optical polarization division multiplexing (PDM) and wireless multiple input multiple output (MIMO) spatial multiplexing techniques. In our experiment, a training-based zero forcing (ZF) channel estimation algorithm is designed to compensate the polarization rotation and wireless multipath fading. A 797 Mb/s net data rate QPSK-OFDM signal with error free (<1 × 10(5)) performance and a 1.59 Gb/s net data rate 16QAM-OFDM signal with BER performance of 1.2 × 10(2) are achieved after transmission of 22.8 km single mode fiber followed by 3 m and 1 m air distances, respectively.

Pharmacodynamic Modeling of Bacillary Elimination Rates and Detection of Bacterial Lipid Bodies in Sputum to Predict and Understand Outcomes in Treatment of Pulmonary Tuberculosis

PubMed Central

Sloan, Derek J.; Mwandumba, Henry C.; Garton, Natalie J.; Khoo, Saye H.; Butterworth, Anthony E.; Allain, Theresa J.; Heyderman, Robert S.; Corbett, Elizabeth L.; Barer, Mike R.; Davies, Geraint R.

2015-01-01

Background. Antibiotic-tolerant bacterial persistence prevents treatment shortening in drug-susceptible tuberculosis, and accumulation of intracellular lipid bodies has been proposed to identify a persister phenotype of Mycobacterium tuberculosis cells. In Malawi, we modeled bacillary elimination rates (BERs) from sputum cultures and calculated the percentage of lipid body–positive acid-fast bacilli (%LB + AFB) on sputum smears. We assessed whether these putative measurements of persistence predict unfavorable outcomes (treatment failure/relapse). Methods. Adults with pulmonary tuberculosis received standard 6-month therapy. Sputum samples were collected during the first 8 weeks for serial sputum colony counting (SSCC) on agar and time-to positivity (TTP) measurement in mycobacterial growth indicator tubes. BERs were extracted from nonlinear and linear mixed-effects models, respectively, fitted to these datasets. The %LB + AFB counts were assessed by fluorescence microscopy. Patients were followed until 1 year posttreatment. Individual BERs and %LB + AFB counts were related to final outcomes. Results. One hundred and thirty-three patients (56% HIV coinfected) participated, and 15 unfavorable outcomes were reported. These were inversely associated with faster sterilization phase bacillary elimination from the SSCC model (odds ratio [OR], 0.39; 95% confidence interval [CI], .22–.70) and a faster BER from the TTP model (OR, 0.71; 95% CI, .55–.94). Higher %LB + AFB counts on day 21–28 were recorded in patients who suffered unfavorable final outcomes compared with those who achieved stable cure (P = .008). Conclusions. Modeling BERs predicts final outcome, and high %LB + AFB counts 3–4 weeks into therapy may identify a persister bacterial phenotype. These methods deserve further evaluation as surrogate endpoints for clinical trials. PMID:25778753

DNA Polymerase β as a Novel Target for Chemotherapeutic Intervention of Colorectal Cancer

PubMed Central

Jaiswal, Aruna S.; Banerjee, Sanjeev; Aneja, Ritu; Sarkar, Fazlul H.; Ostrov, David A.; Narayan, Satya

2011-01-01

Chemoprevention presents a major strategy for the medical management of colorectal cancer. Most drugs used for colorectal cancer therapy induce DNA-alkylation damage, which is primarily repaired by the base excision repair (BER) pathway. Thus, blockade of BER pathway is an attractive option to inhibit the spread of colorectal cancer. Using an in silico approach, we performed a structure-based screen by docking small-molecules onto DNA polymerase β (Pol-β) and identified a potent anti-Pol-β compound, NSC-124854. Our goal was to examine whether NSC-124854 could enhance the therapeutic efficacy of DNA-alkylating agent, Temozolomide (TMZ), by blocking BER. First, we determined the specificity of NSC-124854 for Pol-β by examining in vitro activities of APE1, Fen1, DNA ligase I, and Pol-β-directed single nucleotide (SN)- and long-patch (LP)-BER. Second, we investigated the effect of NSC-124854 on the efficacy of TMZ to inhibit the growth of mismatch repair (MMR)-deficient and MMR-proficient colon cancer cell lines using in vitro clonogenic assays. Third, we explored the effect of NSC-124854 on TMZ-induced in vivo tumor growth inhibition of MMR-deficient and MMR-proficient colonic xenografts implanted in female homozygous SCID mice. Our data showed that NSC-124854 has high specificity to Pol-β and blocked Pol-β-directed SN- and LP-BER activities in in vitro reconstituted system. Furthermore, NSC-124854 effectively induced the sensitivity of TMZ to MMR-deficient and MMR-proficient colon cancer cells both in vitro cell culture and in vivo xenograft models. Our findings suggest a potential novel strategy for the development of highly specific structure-based inhibitor for the prevention of colonic tumor progression. PMID:21311763

Coding gains and error rates from the Big Viterbi Decoder

NASA Technical Reports Server (NTRS)

Onyszchuk, I. M.

1991-01-01

A prototype hardware Big Viterbi Decoder (BVD) was completed for an experiment with the Galileo Spacecraft. Searches for new convolutional codes, studies of Viterbi decoder hardware designs and architectures, mathematical formulations, and decompositions of the deBruijn graph into identical and hierarchical subgraphs, and very large scale integration (VLSI) chip design are just a few examples of tasks completed for this project. The BVD bit error rates (BER), measured from hardware and software simulations, are plotted as a function of bit signal to noise ratio E sub b/N sub 0 on the additive white Gaussian noise channel. Using the constraint length 15, rate 1/4, experimental convolutional code for the Galileo mission, the BVD gains 1.5 dB over the NASA standard (7,1/2) Maximum Likelihood Convolution Decoder (MCD) at a BER of 0.005. At this BER, the same gain results when the (255,233) NASA standard Reed-Solomon decoder is used, which yields a word error rate of 2.1 x 10(exp -8) and a BER of 1.4 x 10(exp -9). The (15, 1/6) code to be used by the Cometary Rendezvous Asteroid Flyby (CRAF)/Cassini Missions yields 1.7 dB of coding gain. These gains are measured with respect to symbols input to the BVD and increase with decreasing BER. Also, 8-bit input symbol quantization makes the BVD resistant to demodulated signal-level variations which may cause higher bandwidth than the NASA (7,1/2) code, these gains are offset by about 0.1 dB of expected additional receiver losses. Coding gains of several decibels are possible by compressing all spacecraft data.

The NASA Science Internet: An integrated approach to networking

NASA Technical Reports Server (NTRS)

Rounds, Fred

1991-01-01

An integrated approach to building a networking infrastructure is an absolute necessity for meeting the multidisciplinary science networking requirements of the Office of Space Science and Applications (OSSA) science community. These networking requirements include communication connectivity between computational resources, databases, and library systems, as well as to other scientists and researchers around the world. A consolidated networking approach allows strategic use of the existing science networking within the Federal government, and it provides networking capability that takes into consideration national and international trends towards multivendor and multiprotocol service. It also offers a practical vehicle for optimizing costs and maximizing performance. Finally, and perhaps most important to the development of high speed computing is that an integrated network constitutes a focus for phasing to the National Research and Education Network (NREN). The NASA Science Internet (NSI) program, established in mid 1988, is structured to provide just such an integrated network. A description of the NSI is presented.

Sitzungsberichte der Heidelberger Akademie der Wissenschaften> " Jahrgang 1987/88 Sitzungsber.Heidelberg 87/88 " Aktive Galaxien

NASA Astrophysics Data System (ADS)

Elsässer, Hans

Die Aktivität von Galaxien ist eine relativ seltene und offenbar kurzlebige Erscheinung, die sich vor allem durch eine verstärkte Abstrahlung, oft über das ganze Spektrum hinweg, vom Röntgen- bis zum Radiobereich, bemerkbar macht. Dabei wird auf neue Befunde an Infrarotgalaxien eingegangen, die dafür sprechen, daß die gravitative Wechselwirkung zwischen Galaxien eine wesentliche Rolle spielt. Das Buch ist eine zusammenfassende Darstellung des heutigen Kenntnisstandes über "Aktive Galaxien", ein Thema, das gegenwärtig im Zentrum des astronomischen Interesses und der aktuellen Forschung steht. Nach einem Überblick über die seit längerem bekannten Phänomene und die Probleme ihrer Deutung wird auf neue, am Max-Planck-Institut für Astronomie mit den Teleskopen der Calar Alto Sternwarte (Südspanien) gewonnene, Ergebnisse eingegangen.

Performance improvement of optical wireless communication through fog with a decision feedback equalizer.

PubMed

Aharonovich, Marius; Arnon, Shlomi

2005-08-01

Optical wireless communication (OWC) systems use the atmosphere as a propagation medium. However, a common problem is that from time to time moderate cloud and fog emerge between the receiver and the transmitter. These adverse weather conditions impose temporal broadening and power loss on the optical signal, which reduces the digital signal-to-noise ratio (DSNR), produces significant intersymbol interference (ISI), and degrades the communication system's bit error rate (BER) and throughput. We propose and investigate the use of a combined adaptive bandwidth mechanism and decision feedback equalizer (DFE) to mitigate these atmospheric multipath effects. Based on theoretical analysis and simulations of DSNR penalties, BER, and optimum system bandwidths, we show that a DFE improves the outdoor OWC system immunity to ISI in foggy weather while maintaining high throughput and desired low BER.

Survivability Using Controlled Security Services

DTIC Science & Technology

2005-06-01

California, Irvine. ear complexities of either (or both) group public key size or signature size with respect to the number of group mem - bers. These...troduced leak-freedom and immediate-revocation. Correctness: any signature produced by any group mem - ber using Sign must be accepted by Verify...can only convince one or more appointed verifiers of its mem - bership, while no other party can verify membership even if the signer cooperates fully

A CBPR Partnership Increases HIV Testing among Men Who Have Sex with Men (MSM): Outcome Findings from a Pilot Test of the "CyBER/Testing" Internet Intervention

ERIC Educational Resources Information Center

Rhodes, Scott D.; Vissman, Aaron T.; Stowers, Jason; Miller, Cindy; McCoy, Thomas P.; Hergenrather, Kenneth C.; Wilkin, Aimee M.; Reece, Michael; Bachmann, Laura H.; Ore, Addison; Ross, Michael W.; Hendrix, Ellen; Eng, Eugenia

2011-01-01

The Internet has emerged as an important tool for the delivery of health promotion and disease prevention interventions. Our community-based participatory research (CBPR) partnership developed and piloted "CyBER/testing", a culturally congruent intervention designed to promote HIV testing among men who have sex with men (MSM) within existing…

Polarization-insensitive all-optical dual pump-phase transmultiplexing from 2 x 10-GBd OOKs to 10-GBd RZ-QPSK using cross-phase modulation in a passive nonlinear birefringent photonic crystal fiber

NASA Astrophysics Data System (ADS)

Mahmood, Tanvir

Considering the network size, bit rate, spectral and channel capacity limitations, different modulation formats may be selectively used in future optical networks. Although the traditional metropolitan area networks (MANs) still uses the non-return-to-zero on-off keying (NRZ-OOK) modulation format due to its technical simplicity and therefore low cost, QPSK format is more advantageous in spectrally efficient long-haul fiber optic transmission systems because of its constant power envelope, and robustness to various transmission impairments. Consequently, an important problem may arise, in particular how to route the OOK-data streams from MANs to long-haul backbone networks when the state of polarization (SOP) of the remotely generated OOK is unpredictable. Hence, the focus of this dissertation was to investigate a polarization insensitive (PI) all-optical nonlinear optical signal processing (NOSP) method that can be implemented at the network cross-connect (X-connect) to transfer data from a remotely and a locally generated OOK data simultaneously to more effectual QPSK format for long-haul transmission. By utilizing cross-phase modulation (XPM) and inherent birefringence of the device, the work demonstrated, for the first time, PI all-optical data transfer utilizing dual pump-phase transmultiplexing (DPTM) from 2 x 10-GBd OOKs to 10-GBd RZ-QPSK in a passive nonlinear birefringent photonic crystal fiber (PCF). Polarization insensitivity was achieved by scrambling the SOP of the remotely generated OOK pump and launching the locally generated OOK pump and the probe off-axis. To mitigate polarization induced power fluctuations and detrimental effects due to nearby partially degenerate and non-degenerate four wave mixings, an optimum pump-probe detuning was also utilized. The PI DPTM RZ-QPSK demonstrated a pre-amplified receiver sensitivity penalty < 5.5 dB at 10--9 bit-error-rate (BER), relative to relative to the FPGA-precoded RZ-DQPSK baseline in ASE-limited transmission system. The effect of the remotely generated OOK pump OSNR degradation on PI DPTM RZ-QPSK was also investigated and it was established that 10 -9 BER metric was attainable till the remotely generated OOK pump reached the threshold OSNR limit of 34 dB/0.1nm. Finally, DWDM transmission performance of the PI DPTM RZ-QPSK signal was evaluated using 138-km long recirculating loop and it was demonstrated that the PI DPTM RZ-QPSK can be transmitted over 1,500 km before it reached ITU-T G.709 7% HD-FEC overhead limit. This propagation distance was well beyond the transmission requisites of any typical metro network (≈ 600 km). Furthermore, it was demonstrated that, within the threshold limit, OSNR degradation of the remotely generated OOK pump had minimal impact on the transmission distance of the PI DPTM RZ-QPSK before it reached 7% HD-FEC overhead limit.

Microbial polychlorinated biphenyl dechlorination in sediments by electrical stimulation: The effect of adding acetate and nonionic surfactant.

PubMed

Yu, Hui; Wan, Hui; Feng, Chunhua; Yi, Xiaoyun; Liu, Xiaoping; Ren, Yuan; Wei, Chaohai

2017-02-15

The necessity for developing an efficient and cost-effective in situ bioremediation technology for sediments contaminated with polychlorinated biphenyls (PCBs) has prompted the application of low-voltage electrical fields to anaerobic digestion systems. Here we show that the use of a sediment-based bio-electrochemical reactor (BER) poised at a potential of -0.50V (vs. a standard calomel electrode, SCE) substantially enhanced the reduction of 2,3,4,5-tetrachlorobiphenyl (PCB 61) when acetate was added as a carbon source. The addition of surfactant Tween 80 to the BER further accelerated the PCB 61 transformation. The comparative study of closed- and open-circuit reactors demonstrated the enrichment conditions affecting the bacterial community structure, the dominant dechlorination metabolisms, and thus the extent, the rate and the products of the reduction of PCBs. The dominant bacterial dechlorinators detected in the BERs in the presence of acetate and Tween 80 are Dehalogenimonas, Dehalobacter, Sulfuricurvum, Dechloromonas and Geobacter, which should be responsible for PCB dechlorination. This study improves understanding of the key factors influencing dechlorination activity in sediment-based BERs polarized at a low potential, as well as the metabolic mechanisms dominating in the PCB dechlorination process. Copyright © 2016 Elsevier B.V. All rights reserved.

Azo dye degradation pathway and bacterial community structure in biofilm electrode reactors.

PubMed

Cao, Xian; Wang, Hui; Zhang, Shuai; Nishimura, Osamu; Li, Xianning

2018-05-31

In this study, the degradation pathway of the azo dye X-3B was explored in biofilm electrode reactors (BERs). The X-3B and chemical oxygen demand (COD) removal efficiencies were evaluated under different voltages, salinities, and temperatures. The removal efficiencies increased with increasing voltage. Additionally, the BER achieved maximum X-3B removal efficiencies of 66.26% and 75.27% at a NaCl concentration of 0.33 g L -1 and temperature of 32 °C, respectively; it achieved a COD removal efficiency of 75.64% at a NaCl concentration of 0.330 g L -1 . Fourier transform infrared spectrometry and gas chromatography-mass spectrometry analysis indicated that the X-3B biodegradation process first involved the interruption of the conjugated double-bond, resulting in aniline, benzodiazepine substance, triazine, and naphthalene ring formation. These compounds were further degraded into lower-molecular-weight products. From this, the degradation pathway of the azo dye X-3B was proposed in BERs. The relative abundances of the microbial community at the phylum and genus levels were affected by temperature, the presence of electrons, and an anaerobic environment in the BERs. To achieve better removal efficiencies, further studies on the functions of the microorganisms are needed. Copyright © 2018. Published by Elsevier Ltd.

Synthesis and characterization of SiO2/(PMMA/Fe3O4) magnetic nanocomposites.

PubMed

Wang, Zhifei; Guo, Yafei; Li, Song; Sun, Yueming; He, Nongyue

2008-04-01

Magnetic silica nanocomposites (magnetic nanoparticles core coated by silica shell) have the wide promising applications in the biomedical field and usually been prepared based on the famous Stöber process. However, the flocculation of Fe3O4 nanoparticles easily occurs during the silica coating, which limits the amount of magnetic silica particles produced in the Stöber process. In this paper, PMMA/Fe3O4 nanoparticles were used in the Stöber process instead of the "nude" Fe3O4 nanoparticles. And coating Fe3O4 with PMMA polymer beforehand can prevent magnetic nanoparticles from the aggregation that usually comes from the increasing of ionic strength during the hydrolyzation of tetraethoxysilane (TEOS) by the steric hindrance. The results show that the critical concentration of magnetic nanoparticles can increase from 12 mg/L for "nude" Fe3O4 nanoparticles to 3 g/L for PMMA/Fe3O4 nanoparticles during the Stöber process. And before the deposition of silica shell, the surface of PMMA/FeO4 nanoparticles had to be further modified by hydrolyzing them in CH3OH/NH3 x H2O mixture solution, which provides the carboxyl groups on their surface to react further with the silanol groups of silicic acid.

Phase Noise Influence in Long-range Coherent Optical OFDM Systems with Delay Detection, IFFT Multiplexing and FFT Demodulation

NASA Astrophysics Data System (ADS)

Jacobsen, Gunnar; Xu, Tianhua; Popov, Sergei; Sergeyev, Sergey; Zhang, Yimo

2012-12-01

We present a study of the influence of dispersion induced phase noise for CO-OFDM systems using FFT multiplexing/IFFT demultiplexing techniques (software based). The software based system provides a method for a rigorous evaluation of the phase noise variance caused by Common Phase Error (CPE) and Inter-Carrier Interference (ICI) including - for the first time to our knowledge - in explicit form the effect of equalization enhanced phase noise (EEPN). This, in turns, leads to an analytic BER specification. Numerical results focus on a CO-OFDM system with 10-25 GS/s QPSK channel modulation. A worst case constellation configuration is identified for the phase noise influence and the resulting BER is compared to the BER of a conventional single channel QPSK system with the same capacity as the CO-OFDM implementation. Results are evaluated as a function of transmission distance. For both types of systems, the phase noise variance increases significantly with increasing transmission distance. For a total capacity of 400 (1000) Gbit/s, the transmission distance to have the BER < 10-2 for the worst case CO-OFDM design is less than 800 and 460 km, respectively, whereas for a single channel QPSK system it is less than 1400 and 560 km.

Delineating the requirements for spontaneous DNA damage resistance pathways in genome maintenance and viability in Saccharomyces cerevisiae.

PubMed

Morey, Natalie J; Doetsch, Paul W; Jinks-Robertson, Sue

2003-06-01

Cellular metabolic processes constantly generate reactive species that damage DNA. To counteract this relentless assault, cells have developed multiple pathways to resist damage. The base excision repair (BER) and nucleotide excision repair (NER) pathways remove damage whereas the recombination (REC) and postreplication repair (PRR) pathways bypass the damage, allowing deferred removal. Genetic studies in yeast indicate that these pathways can process a common spontaneous lesion(s), with mutational inactivation of any pathway increasing the burden on the remaining pathways. In this study, we examine the consequences of simultaneously compromising three or more of these pathways. Although the presence of a functional BER pathway alone is able to support haploid growth, retention of the NER, REC, or PRR pathway alone is not, indicating that BER is the key damage resistance pathway in yeast and may be responsible for the removal of the majority of either spontaneous DNA damage or specifically those lesions that are potentially lethal. In the diploid state, functional BER, NER, or REC alone can support growth, while PRR alone is insufficient for growth. In diploids, the presence of PRR alone may confer a lethal mutation load or, alternatively, PRR alone may be insufficient to deal with potentially lethal, replication-blocking lesions.

Dielectric loaded surface plasmon waveguides for datacom applications

NASA Astrophysics Data System (ADS)

Weeber, J.-C.; Hassan, K.; Nielsen, M. G.; Pitilakis, A.; Tsilipakos, O.; Kriezis, E. E.; Fatome, J.; Finot, C.; Markey, L.; Albrektsen, O.; Bozhevolnyi, S. I.; Dereux, A.

2012-04-01

We rst report on design, fabrication and characterizations of thermally-controlled plasmonic routers relying on the interference of a plasmonic and a photonic mode supported by wide enough dielectric loaded waveguides. We show that, by owing a current through the gold lm on which the dielectric waveguides are deposited, the length of the beating created by the interference of the two modes can be controlled accurately. By operating such a plasmonic dual-mode interferometer switch, symmetric extinction ratio of 7dB are obtained at the output ports of a 2x2 router. Next, we demonstrate ber-to-ber characterizations of stand-alone dielectric loaded surface plasmon waveguide (DLSPPW) devices by using grating couplers. The couplers are comprised of dielectric loaded gratings with carefully chosen periods and duty-cycles close to 0.5. We show that insertion loss below 10dB per coupler can be achieved with optimized gratings. This coupling scheme is used to operate Bit-Error-Rate (BER) measurements for the transmission of a 10Gbits/s signal along a stand-alone straight DLSPPW. We show in particular that these waveguides introduce a rather small BER power penalty (below 1dB) demonstrating the suitability of this plasmonic waveguiding platform for high-bit rate transmission.

Array design considerations for exploitation of stable weakly dispersive modal pulses in the deep ocean

NASA Astrophysics Data System (ADS)

Udovydchenkov, Ilya A.

2017-07-01

Modal pulses are broadband contributions to an acoustic wave field with fixed mode number. Stable weakly dispersive modal pulses (SWDMPs) are special modal pulses that are characterized by weak dispersion and weak scattering-induced broadening and are thus suitable for communications applications. This paper investigates, using numerical simulations, receiver array requirements for recovering information carried by SWDMPs under various signal-to-noise ratio conditions without performing channel equalization. Two groups of weakly dispersive modal pulses are common in typical mid-latitude deep ocean environments: the lowest order modes (typically modes 1-3 at 75 Hz), and intermediate order modes whose waveguide invariant is near-zero (often around mode 20 at 75 Hz). Information loss is quantified by the bit error rate (BER) of a recovered binary phase-coded signal. With fixed receiver depths, low BERs (less than 1%) are achieved at ranges up to 400 km with three hydrophones for mode 1 with 90% probability and with 34 hydrophones for mode 20 with 80% probability. With optimal receiver depths, depending on propagation range, only a few, sometimes only two, hydrophones are often sufficient for low BERs, even with intermediate mode numbers. Full modal resolution is unnecessary to achieve low BERs. Thus, a flexible receiver array of autonomous vehicles can outperform a cabled array.

Proximity to AGCT sequences dictates MMR-independent versus MMR-dependent mechanisms for AID-induced mutation via UNG2

PubMed Central

Thientosapol, Eddy Sanchai; Sharbeen, George; Lau, K.K. Edwin; Bosnjak, Daniel; Durack, Timothy; Stevanovski, Igor; Weninger, Wolfgang

2017-01-01

Abstract AID deaminates C to U in either strand of Ig genes, exclusively producing C:G/G:C to T:A/A:T transition mutations if U is left unrepaired. Error-prone processing by UNG2 or mismatch repair diversifies mutation, predominantly at C:G or A:T base pairs, respectively. Here, we show that transversions at C:G base pairs occur by two distinct processing pathways that are dictated by sequence context. Within and near AGCT mutation hotspots, transversion mutation at C:G was driven by UNG2 without requirement for mismatch repair. Deaminations in AGCT were refractive both to processing by UNG2 and to high-fidelity base excision repair (BER) downstream of UNG2, regardless of mismatch repair activity. We propose that AGCT sequences resist faithful BER because they bind BER-inhibitory protein(s) and/or because hemi-deaminated AGCT motifs innately form a BER-resistant DNA structure. Distal to AGCT sequences, transversions at G were largely co-dependent on UNG2 and mismatch repair. We propose that AGCT-distal transversions are produced when apyrimidinic sites are exposed in mismatch excision patches, because completion of mismatch repair would require bypass of these sites. PMID:28039326

200-GHz and 50-GHz AWG channelized linewidth dependent transmission of weak-resonant-cavity FPLD injection-locked by spectrally sliced ASE.

PubMed

Lin, Gong-Ru; Cheng, Tzu-Kang; Chi, Yu-Chieh; Lin, Gong-Cheng; Wang, Hai-Lin; Lin, Yi-Hong

2009-09-28

In a weak-resonant-cavity Fabry-Perot laser diode (WRC-FPLD) based DWDM-PON system with an array-waveguide-grating (AWG) channelized amplified spontaneous emission (ASE) source located at remote node, we study the effect of AWG filter bandwidth on the transmission performances of the 1.25-Gbit/s directly modulated WRC-FPLD transmitter under the AWG channelized ASE injection-locking. With AWG filters of two different channel spacings at 50 and 200 GHz, several characteristic parameters such as interfered reflection, relatively intensity noise, crosstalk reduction, side-mode-suppressing ratio and power penalty of BER effect of the WRC-FPLD transmitted data are compared. The 200-GHz AWG filtered ASE injection minimizes the noises of WRC-FPLD based ONU transmitter, improving the power penalty of upstream data by -1.6 dB at BER of 10(-12). In contrast, the 50-GHz AWG channelized ASE injection fails to promote better BER but increases the power penalty by + 1.5 dB under back-to-back transmission. A theoretical modeling elucidates that the BER degradation up to 4 orders of magnitude between two injection cases is mainly attributed to the reduction on ASE injection linewidth, since which concurrently degrades the signal-to-noise and extinction ratios of the transmitted data stream.

Average BER and outage probability of the ground-to-train OWC link in turbulence with rain

NASA Astrophysics Data System (ADS)

Zhang, Yixin; Yang, Yanqiu; Hu, Beibei; Yu, Lin; Hu, Zheng-Da

2017-09-01

The bit-error rate (BER) and outage probability of optical wireless communication (OWC) link for the ground-to-train of the curved track in turbulence with rain is evaluated. Considering the re-modulation effects of raining fluctuation on optical signal modulated by turbulence, we set up the models of average BER and outage probability in the present of pointing errors, based on the double inverse Gaussian (IG) statistical distribution model. The numerical results indicate that, for the same covered track length, the larger curvature radius increases the outage probability and average BER. The performance of the OWC link in turbulence with rain is limited mainly by the rain rate and pointing errors which are induced by the beam wander and train vibration. The effect of the rain rate on the performance of the link is more severe than the atmospheric turbulence, but the fluctuation owing to the atmospheric turbulence affects the laser beam propagation more greatly than the skewness of the rain distribution. Besides, the turbulence-induced beam wander has a more significant impact on the system in heavier rain. We can choose the size of transmitting and receiving apertures and improve the shockproof performance of the tracks to optimize the communication performance of the system.

The Identification of Major Factors in the Deployment of a Science DMZ at Small Institutions

ERIC Educational Resources Information Center

Valcourt, Scott A.

2017-01-01

The Science DMZ is a network research tool offering superior large science data transmission between two locations. Through a network design that places the Science DMZ at the edge of the campus network, the Science DMZ defines a network path that avoids packet inspecting devices (firewalls, packet shapers) and produces near line-rate transmission…

Symposium Connects Government Problems with State of the Art Network Science Research

DTIC Science & Technology

2015-10-16

Symposium Connects Government Problems with State-of-the- Art Network Science Research By Rajmonda S. Caceres and Benjamin A. Miller Network...the US Gov- ernment, and match these with the state-of-the- art models and techniques developed in the network science research community. Since its... science has grown significantly in the last several years as a field at the intersec- tion of mathematics, computer science , social science , and engineering

The Science DMZ: A Network Design Pattern for Data-Intensive Science

DOE PAGES

Dart, Eli; Rotman, Lauren; Tierney, Brian; ...

2014-01-01

The ever-increasing scale of scientific data has become a significant challenge for researchers that rely on networks to interact with remote computing systems and transfer results to collaborators worldwide. Despite the availability of high-capacity connections, scientists struggle with inadequate cyberinfrastructure that cripples data transfer performance, and impedes scientific progress. The Science DMZ paradigm comprises a proven set of network design patterns that collectively address these problems for scientists. We explain the Science DMZ model, including network architecture, system configuration, cybersecurity, and performance tools, that creates an optimized network environment for science. We describe use cases from universities, supercomputing centers andmore » research laboratories, highlighting the effectiveness of the Science DMZ model in diverse operational settings. In all, the Science DMZ model is a solid platform that supports any science workflow, and flexibly accommodates emerging network technologies. As a result, the Science DMZ vastly improves collaboration, accelerating scientific discovery.« less

The effect of electronic networking on preservice elementary teachers' science teaching self-efficacy and attitude towards science teaching

NASA Astrophysics Data System (ADS)

Mathew, Nishi Mary

Preservice elementary teachers' science teaching efficacy and attitude towards science teaching are important determinants of whether and how they will teach science in their classrooms. Preservice teachers' understanding of science and science teaching experiences have an impact on their beliefs about their ability to teach science. This study had a quasi-experimental pretest-posttest control group design (N = 60). Preservice elementary teachers in this study were networked through the Internet (using e-mail, newsgroups, listserv, world wide web access and electronic mentoring) during their science methods class and student practicum. Electronic networking provides a social context in which to learn collaboratively, share and reflect upon science teaching experiences and practices, conduct tele-research effectively, and to meet the demands of student teaching through peer support. It was hoped that the activities over the electronic networks would provide them with positive and helpful science learning and teaching experiences. Self-efficacy was measured using a 23-item Likert scale instrument, the Science Teaching Efficacy Belief Instrument, Form-B (STEBI-B). Attitude towards science teaching was measured using the Revised Science Attitude Scale (RSAS). Analysis of covariance was used to analyze the data, with pretest scores as the covariate. Findings of this study revealed that prospective elementary teachers in the electronically networked group had better science teaching efficacy and personal science teaching efficacy as compared to the non-networked group of preservice elementary teachers. The science teaching outcome expectancy of prospective elementary teachers in the networked group was not greater than that of the prospective teachers in the non-networked group (at p < 0.05). Attitude towards science teaching was not significantly affected by networking. However, this is surmised to be related to the duration of the study. Information about the experiences of the participants in this study was also collected through interview, and inventories. Findings from the interview data revealed that prospective teachers benefited from the interactions with peers, science mentors, and science methods instructors during student teaching. Students who did not have access to computers noted that time was a constraint in the use of the electronic networks.

The Earth Science Research Network as Seen Through Network Analysis of the AGU

NASA Astrophysics Data System (ADS)

Narock, T.; Hasnain, S.; Stephan, R.

2017-12-01

Scientometrics is the science of science. Scientometric research includes measurements of impact, mapping of scientific fields, and the production of indicators for use in policy and management. We have leveraged network analysis in a scientometric study of the American Geophysical Union (AGU). Data from the AGU's Linked Data Abstract Browser was used to create a visualization and analytics tools to explore the Earth science's research network. Our application applies network theory to look at network structure within the various AGU sections, identify key individuals and communities related to Earth science topics, and examine multi-disciplinary collaboration across sections. Opportunities to optimize Earth science output, as well as policy and outreach applications, are discussed.

Networks in cognitive science.

PubMed

Baronchelli, Andrea; Ferrer-i-Cancho, Ramon; Pastor-Satorras, Romualdo; Chater, Nick; Christiansen, Morten H

2013-07-01

Networks of interconnected nodes have long played a key role in Cognitive Science, from artificial neural networks to spreading activation models of semantic memory. Recently, however, a new Network Science has been developed, providing insights into the emergence of global, system-scale properties in contexts as diverse as the Internet, metabolic reactions, and collaborations among scientists. Today, the inclusion of network theory into Cognitive Sciences, and the expansion of complex-systems science, promises to significantly change the way in which the organization and dynamics of cognitive and behavioral processes are understood. In this paper, we review recent contributions of network theory at different levels and domains within the Cognitive Sciences. Copyright © 2013 Elsevier Ltd. All rights reserved.

Breast Cancer and Estrogen Biosynthesis in Adipose Tissue

DTIC Science & Technology

1998-10-01

transferred to a nitrocellulose mem - brane. The transferred proteins were subjected to a denaturation/rena- turation process and hybridized to the 32P...aromatase expression in adipose tissue has been recently observed to be regulated by mem - bers of the interleukin-6 (IL-6) cytokine family. Based on...shown in human adipose stromal cells that the stimulatory effects of serum on aromatase expression can be mimicked by mem - bers of the interleukin-6

Experimental results of 5-Gbps free-space coherent optical communications with adaptive optics

NASA Astrophysics Data System (ADS)

Chen, Mo; Liu, Chao; Rui, Daoman; Xian, Hao

2018-07-01

In a free-space optical communication system with fiber optical components, the received signal beam must be coupled into a single-mode fiber (SMF) before being amplified and detected. The impacts analysis of tracking errors and wavefront distortion on SMF coupling show that under the condition of relatively strong turbulence, only the tracking errors compensation is not enough, and turbulence wavefront aberration is required to be corrected. Based on our previous study and design of SMF coupling system with a 137-element continuous surface deformable mirror AO unit, we perform an experiment of a 5-Gbps Free-space Coherent Optical Communication (FSCOC) system, in which the eye pattern and Bit-error Rate (BER) are displayed. The comparative results are shown that the influence of the atmospheric is fatal in FSCOC systems. The BER of coherent communication is under 10-6 with AO compensation, which drops significantly compared with the BER without AO correction.

Channel correlation and BER performance analysis of coherent optical communication systems with receive diversity over moderate-to-strong non-Kolmogorov turbulence.

PubMed

Fu, Yulong; Ma, Jing; Tan, Liying; Yu, Siyuan; Lu, Gaoyuan

2018-04-10

In this paper, new expressions of the channel-correlation coefficient and its components (the large- and small-scale channel-correlation coefficients) for a plane wave are derived for a horizontal link in moderate-to-strong non-Kolmogorov turbulence using a generalized effective atmospheric spectrum which includes finite-turbulence inner and outer scales and high-wave-number "bump". The closed-form expression of the average bit error rate (BER) of the coherent free-space optical communication system is derived using the derived channel-correlation coefficients and an α-μ distribution to approximate the sum of the square root of arbitrarily correlated Gamma-Gamma random variables. Analytical results are provided to investigate the channel correlation and evaluate the average BER performance. The validity of the proposed approximation is illustrated by Monte Carlo simulations. This work will help with further investigation of the fading correlation in spatial diversity systems.

Sitzungsberichte der Heidelberger Akademie der Wissenschaften¬Jahrgang 1987/88 Sitzungsber.Heidelberg 87/88¬Aktive Galaxien

NASA Astrophysics Data System (ADS)

Elsässer, Hans

Die Aktivität von Galaxien ist eine relativ seltene und offenbar kurzlebige Erscheinung, die sich vor allem durch eine verstärkte Abstrahlung, oft über das ganze Spektrum hinweg, vom Röntgen- bis zum Radiobereich, bemerkbar macht. Dabei wird auf neue Befunde an Infrarotgalaxien eingegangen, die dafür sprechen, daß die gravitative Wechselwirkung zwischen Galaxien eine wesentliche Rolle spielt. Das Buch ist eine zusammenfassende Darstellung des heutigen Kenntnisstandes über "Aktive Galaxien", ein Thema, das gegenwärtig im Zentrum des astronomischen Interesses und der aktuellen Forschung steht. Nach einem Überblick über die seit längerem bekannten Phänomene und die Probleme ihrer Deutung wird auf neue, am Max-Planck-Institut für Astronomie mit den Teleskopen der Calar Alto Sternwarte (Südspanien) gewonnene, Ergebnisse eingegangen.

Bildbasierte Navigation eines mobilen Roboters mittels omnidirektionaler und schwenkbarer Kamera

NASA Astrophysics Data System (ADS)

Nierobisch, Thomas; Hoffmann, Frank; Krettek, Johannes; Bertram, Torsten

Dieser Beitrag präsentiert einen neuartigen Ansatz zur entkoppelten Regelung der Kamera-Blickrichtung und der Bewegung eines mobilen Roboters im Kontext der bildbasierten Navigation. Eine schwenkbare monokulare Kamera hält unabhängig von der Roboterbewegung die relevanten Merkmale für die Navigation im Sichtfeld. Die Entkopplung der Kamerablickrichtung von der eigentlichen Roboterbewegung wird durch die Projektion der Merkmale auf eine virtuelle Bildebene realisiert. In der virtuellen Bildebene hängt die Ausprägung der visuellen Merkmale für die bildbasierte Regelung nur von der Roboterposition ab und ist unabhängig gegenüber der tatsächlichen Blickrichtung der Kamera. Durch die Schwenkbarkeit der monokularen Kamera wird der Arbeitsbereich, über dem sich ein Referenzbild zur bildbasierten Regelung eignet, gegenüber einer statischen Kamera signifikant vergrößert. Dies ermöglicht die Navigation auch in texturarmen Umgebungen, die wenig verwertbare Textur- und Strukturmerkmale aufweisen.

Underwater wireless optical MIMO system with spatial modulation and adaptive power allocation

NASA Astrophysics Data System (ADS)

Huang, Aiping; Tao, Linwei; Niu, Yilong

2018-04-01

In this paper, we investigate the performance of underwater wireless optical multiple-input multiple-output communication system combining spatial modulation (SM-UOMIMO) with flag dual amplitude pulse position modulation (FDAPPM). Channel impulse response for coastal and harbor ocean water links are obtained by Monte Carlo (MC) simulation. Moreover, we obtain the closed-form and upper bound average bit error rate (BER) expressions for receiver diversity including optical combining, equal gain combining and selected combining. And a novel adaptive power allocation algorithm (PAA) is proposed to minimize the average BER of SM-UOMIMO system. Our numeric results indicate an excellent match between the analytical results and numerical simulations, which confirms the accuracy of our derived expressions. Furthermore, the results show that adaptive PAA outperforms conventional fixed factor PAA and equal PAA obviously. Multiple-input single-output system with adaptive PAA obtains even better BER performance than MIMO one, at the same time reducing receiver complexity effectively.

Simulation realization of 2-D wavelength/time system utilizing MDW code for OCDMA system

NASA Astrophysics Data System (ADS)

Azura, M. S. A.; Rashidi, C. B. M.; Aljunid, S. A.; Endut, R.; Ali, N.

2017-11-01

This paper presents a realization of Wavelength/Time (W/T) Two-Dimensional Modified Double Weight (2-D MDW) code for Optical Code Division Multiple Access (OCDMA) system based on Spectral Amplitude Coding (SAC) approach. The MDW code has the capability to suppress Phase-Induce Intensity Noise (PIIN) and minimizing the Multiple Access Interference (MAI) noises. At the permissible BER 10-9, the 2-D MDW (APD) had shown minimum effective received power (Psr) = -71 dBm that can be obtained at the receiver side as compared to 2-D MDW (PIN) only received -61 dBm. The results show that 2-D MDW (APD) has better performance in achieving same BER with longer optical fiber length and with less received power (Psr). Also, the BER from the result shows that MDW code has the capability to suppress PIIN ad MAI.

RLS Channel Estimation with Adaptive Forgetting Factor for DS-CDMA Frequency-Domain Equalization

NASA Astrophysics Data System (ADS)

Kojima, Yohei; Tomeba, Hiromichi; Takeda, Kazuaki; Adachi, Fumiyuki

Frequency-domain equalization (FDE) based on the minimum mean square error (MMSE) criterion can increase the downlink bit error rate (BER) performance of DS-CDMA beyond that possible with conventional rake combining in a frequency-selective fading channel. FDE requires accurate channel estimation. Recently, we proposed a pilot-assisted channel estimation (CE) based on the MMSE criterion. Using MMSE-CE, the channel estimation accuracy is almost insensitive to the pilot chip sequence, and a good BER performance is achieved. In this paper, we propose a channel estimation scheme using one-tap recursive least square (RLS) algorithm, where the forgetting factor is adapted to the changing channel condition by the least mean square (LMS)algorithm, for DS-CDMA with FDE. We evaluate the BER performance using RLS-CE with adaptive forgetting factor in a frequency-selective fast Rayleigh fading channel by computer simulation.

Iterative Code-Aided ML Phase Estimation and Phase Ambiguity Resolution

NASA Astrophysics Data System (ADS)

Wymeersch, Henk; Moeneclaey, Marc

2005-12-01

As many coded systems operate at very low signal-to-noise ratios, synchronization becomes a very difficult task. In many cases, conventional algorithms will either require long training sequences or result in large BER degradations. By exploiting code properties, these problems can be avoided. In this contribution, we present several iterative maximum-likelihood (ML) algorithms for joint carrier phase estimation and ambiguity resolution. These algorithms operate on coded signals by accepting soft information from the MAP decoder. Issues of convergence and initialization are addressed in detail. Simulation results are presented for turbo codes, and are compared to performance results of conventional algorithms. Performance comparisons are carried out in terms of BER performance and mean square estimation error (MSEE). We show that the proposed algorithm reduces the MSEE and, more importantly, the BER degradation. Additionally, phase ambiguity resolution can be performed without resorting to a pilot sequence, thus improving the spectral efficiency.

EVIDENCE FOR BASE EXCISION REPAIR PROCESSING OF DNA INTERSTRAND CROSSLINKS

PubMed Central

Kothandapani, Anbarasi; Patrick, Steve M

2013-01-01

Many bifunctional alkylating agents and anticancer drugs exert their cytotoxicity by producing cross links between the two complementary strands of DNA, termed interstrand crosslinks (ICLs). This blocks the strand separating processes during DNA replication and transcription, which can lead to cell cycle arrest and apoptosis. Cells use multiple DNA repair systems to eliminate the ICLs. Concerted action of repair proteins involved in Nucleotide Excision Repair and Homologous Recombination pathways are suggested to play a key role in the ICL repair. However, recent studies indicate a possible role for Base Excision Repair (BER) in mediating the cytotoxicity of ICL inducing agents in mammalian cells. Elucidating the mechanism of BER mediated modulation of ICL repair would help in understanding the recognition and removal of ICLs and aid in the development of potential therapeutic agents. In this review, the influence of BER proteins on ICL DNA repair and the possible mechanisms of action are discussed. PMID:23219605

Application of the Bulgarian emergency response system in case of nuclear accident in environmental assessment study

NASA Astrophysics Data System (ADS)

Syrakov, Dimiter; Veleva, Blagorodka; Georgievs, Emilia; Prodanova, Maria; Slavov, Kiril; Kolarova, Maria

2014-05-01

The development of the Bulgarian Emergency Response System (BERS) for short term forecast in case of accidental radioactive releases to the atmosphere has been started in the mid 1990's [1]. BERS comprises of two main parts - operational and accidental, for two regions 'Europe' and 'Northern Hemisphere'. The operational part runs automatically since 2001 using the 72 hours meteorological forecast from DWD Global model, resolution in space of 1.5o and in time - 12 hours. For specified Nuclear power plants (NPPs), 3 days trajectories are calculated and presented on NIMH's specialized Web-site (http://info.meteo.bg/ews/). The accidental part is applied when radioactive releases are reported or in case of emergency exercises. BERS is based on numerical weather forecast information and long-range dispersion model accounting for the transport, dispersion, and radioactive transformations of pollutants. The core of the accidental part of the system is the Eulerian 3D dispersion model EMAP calculating concentration and deposition fields [2]. The system is upgraded with a 'dose calculation module' for estimation of the prognostic dose fields of 31 important radioactive gaseous and aerosol pollutants. The prognostic doses significant for the early stage of a nuclear accident are calculated as follows: the effective doses from external irradiation (air submersion + ground shinning); effective dose from inhalation; summarized effective dose and absorbed thyroid dose [3]. The output is given as 12, 24, 36, 48, 60 and 72 hours prognostic dose fields according the updated meteorology. The BERS was upgraded to simulate the dispersion of nuclear materials from Fukushima NPP [4], and results were presented in NIMH web-site. In addition BERS took part in the respective ENSEMBLE exercises to model 131I and 137Cs in Fukushima source term. In case of governmental request for expertise BERS was applied for environmental impact assessment of hypothetical accidental transboundary radioactive pollution. The consequences were estimated based on the worst emission scenario for the existing basic reactor type, selection of real meteorological forecast conditions, favoring the direct transport of the contaminated air masses to the territory of the country in consideration. In the present work BERS is used to estimate the worst case accidental scenario impact from a possible new unit of Paks Nuclear Power Plant, Hungary over the territory of Bulgaria. 1. D.Syrakov, M.Prodanova, 1998, Atmospheric Environment, 32 (24), 4367-4375. 2. D. Syrakov, M. Prodanova, K. Slavov, Inernationsal J. Environment and Pollution, 20, 1-6 (2003) 286-296. 3. D. Syrakov, B. Veleva, M. Prodanova, T. Popova, M. Kolarova, Journal of Environmental Radioactivity 100 (2009) 151-156. 4. D.Syrakov, M Prodanova, J. Intern. Sci. Publ.: Ecology & Safety Vol. 6 Part 1 (2011) 94-102. www.scientific-publications.net.

All-optical VPN utilizing DSP-based digital orthogonal filters access for PONs

NASA Astrophysics Data System (ADS)

Zhang, Xiaoling; Zhang, Chongfu; Chen, Chen; Jin, Wei; Qiu, Kun

2018-04-01

Utilizing digital filtering-enabled signal multiplexing and de-multiplexing, a cost-effective all-optical virtual private network (VPN) system is proposed, for the first time to our best knowledge, in digital filter multiple access passive optical networks (DFMA-PONs). Based on the DFMA technology, the proposed system can be easily designed to meet the requirements of next generation network's flexibility, elasticity, adaptability and compatibility. Through dynamic digital filter allocation and recycling, the proposed all-optical VPN system can provide dynamic establishments and cancellations of multiple VPN communications with arbitrary traffic volumes. More importantly, due to the employment of DFMA technology, the system is not limited to a fixed signal format and different signal formats such as pulse amplitude modulation (PAM), quadrature amplitude modulation (QAM) and orthogonal frequency division multiplexing (OFDM) can be used. Moreover, one transceiver is sufficient to simultaneously transmit upstream (US)/VPN data to optical line terminal (OLT) or other VPN optical network units (ONUs), thus leading to great reduction in network constructions and operation expenditures. The proposed all-optical VPN system is demonstrated with the transceiver incorporating the formats of QAM and OFDM, which can be made transparent to downstream (DS), US and VPN communications. The bit error rates (BERs) of DS, US and VPN for OFDM signals are below the forward-error-correction (FEC) limit of 3 . 8 × 10-3 when the received optical powers are about -16.8 dBm, -14.5 dBm and -15.7 dBm, respectively.

Demonstration of flexible multicasting and aggregation functionality for TWDM-PON

NASA Astrophysics Data System (ADS)

Chen, Yuanxiang; Li, Juhao; Zhu, Paikun; Zhu, Jinglong; Tian, Yu; Wu, Zhongying; Peng, Huangfa; Xu, Yongchi; Chen, Jingbiao; He, Yongqi; Chen, Zhangyuan

2017-06-01

The time- and wavelength-division multiplexed passive optical network (TWDM-PON) has been recognized as an attractive solution to provide broadband access for the next-generation networks. In this paper, we propose flexible service multicasting and aggregation functionality for TWDM-PON utilizing multiple-pump four-wave-mixing (FWM) and cyclic arrayed waveguide grating (AWG). With the proposed scheme, multiple TWDM-PON links share a single optical line terminal (OLT), which can greatly reduce the network deployment expense and achieve efficient network resource utilization by load balancing among different optical distribution networks (ODNs). The proposed scheme is compatible with existing TDM-PON infrastructure with fixed-wavelength OLT transmitter, thus smooth service upgrade can be achieved. Utilizing the proposed scheme, we demonstrate a proof-of-concept experiment with 10-Gb/s OOK and 10-Gb/s QPSK orthogonal frequency division multiplexing (OFDM) signal multicasting and aggregating to seven PON links. Compared with back-to-back (BTB) channel, the newly generated multicasting OOK signal and OFDM signal have power penalty of 1.6 dB and 2 dB at the BER of 10-3, respectively. For the aggregation of multiple channels, no obvious power penalty is observed. What is more, to verify the flexibility of the proposed scheme, we reconfigure the wavelength selective switch (WSS) and adjust the number of pumps to realize flexible multicasting functionality. One to three, one to seven, one to thirteen and one to twenty-one multicasting are achieved without modifying OLT structure.

Polar Research with Unmanned Aircraft and Tethered Balloons

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ivey, M; Petty, R; Desilets, D

2014-01-24

The Arctic is experiencing rapid climate change, with nearly double the rate of surface warming observed elsewhere on the planet. While various positive feedback mechanisms have been suggested, the reasons for Arctic amplification are not well understood, nor are the impacts to the global carbon cycle well quantified. Additionally, there are uncertainties associated with the complex interactions between Earth’s surface and the atmosphere. Elucidating the causes and consequences of Arctic warming is one of the many goals of the Climate and Environmental Sciences Division (CESD) of the U.S. Department of Energy’s (DOE) Biological and Environmental Research (BER) program, and ismore » part of the larger CESD initiative to develop a robust predictive understanding of Earth’s climate system.« less

Mendel at the sesquicentennial of 'Versuche über Pflanzen-Hybriden' (1865): The root of the biggest legend in the history of science.

PubMed

Tanghe, Koen B

2015-06-01

In 1965, Mendel was still celebrated as the undisputed founder of genetics. In the ensuing 50 years, scholars questioned and undermined this traditional interpretation of his experiments with hybrid plants, without, however, managing to replace it: at the sesquicentennial of the presentation of his 'Versuche' (1865), the Moravian friar remains, to a vast majority, the heroic Father of genetics or at least some kind of geneticist. This exceptionally inert myth is nourished by ontological intuitions but can only continue to flourish, thanks to a long-standing conceptual void in the historiography of biology. It is merely a symptom of this more fundamental problem. Copyright © 2015 Elsevier Ltd. All rights reserved.

Instrumentation for Scientific Computing in Neural Networks, Information Science, Artificial Intelligence, and Applied Mathematics.

DTIC Science & Technology

1987-10-01

include Security Classification) Instrumentation for scientific computing in neural networks, information science, artificial intelligence, and...instrumentation grant to purchase equipment for support of research in neural networks, information science, artificail intellignece , and applied mathematics...in Neural Networks, Information Science, Artificial Intelligence, and Applied Mathematics Contract AFOSR 86-0282 Principal Investigator: Stephen

Active Hydrogenation Catalyst with a Structured, Peptide-Based Outer-Coordination Sphere

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jain, Avijita; Buchko, Garry W.; Reback, Matthew L.

2012-10-05

The synthesis, catalytic activity, and structural features of a rhodium-based hydrogenation catalyst containing a phosphine ligand coupled to a 14-residue peptide are reported. Both CD and NMR spectroscopy show that the peptide adopts a helical structure in 1:1:1 TFE/MeCN/H2O that is maintained when the peptide is attached to the ligand and when the ligand is attached to the metal complex. The metal complex hydrogenates aqueous solutions of 3-butenol to 1-butanol at 360 ± 50 turnovers/Rh/h at 294 K. This peptide- based catalyst represents a starting point for developing and characterizing a peptide-based outer-coordination sphere that can be used to introducemore » enzyme-like features into molecular catalysts. This work was funded by the US DOE Basic Energy Sciences, Chemical Sciences, Geoscience and Biosciences Division (AJ, JCL and WJS), the Office of Science Early Career Research Program through the Office of Basic Energy Sciences (GWB, MLR and WJS). Part of the research was conducted at the W.R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by U.S. Department of Energy’s Office of Biolog-ical and Environmental Research (BER) program located at Pacific Northwest National Laboratory (PNNL). PNNL is operated by Battelle for the U.S. Department of Energy.« less

Differential mesocorticolimbic responses to palatable food in binge eating prone and binge eating resistant female rats.

PubMed

Sinclair, Elaine B; Culbert, Kristen M; Gradl, Dana R; Richardson, Kimberlei A; Klump, Kelly L; Sisk, Cheryl L

2015-12-01

Binge eating is a key symptom of many eating disorders (e.g. binge eating disorder, bulimia nervosa, anorexia nervosa binge/purge type), yet the neurobiological underpinnings of binge eating are poorly understood. The mesocorticolimbic reward circuit, including the nucleus accumbens and the medial prefrontal cortex, is likely involved because this circuit mediates the hedonic value and incentive salience of palatable foods (PF). Here we tested the hypothesis that higher propensity for binge eating is associated with a heightened response (i.e., Fos induction) of the nucleus accumbens and medial prefrontal cortex to PF, using an animal model that identifies binge eating prone (BEP) and binge eating resistant (BER) rats. Forty adult female Sprague-Dawley rats were given intermittent access to PF (high fat pellets) 3×/week for 3 weeks. Based on a pattern of either consistently high or consistently low PF consumption across these feeding tests, 8 rats met criteria for categorization as BEP, and 11 rats met criteria for categorization as BER. One week after the final feeding test, BEP and BER rats were either exposed to PF in their home cages or were given no PF in their home cages for 1h prior to perfusion, leading to three experimental groups for the Fos analysis: BEPs given PF, BERs given PF, and a No PF control group. The total number of Fos-immunoreactive (Fos-ir) cells in the nucleus accumbens core and shell, and the cingulate, prelimbic, and infralimbic regions of the medial prefrontal cortex was estimated by stereological analysis. PF induced higher Fos expression in the nucleus accumbens shell and core and in the prelimbic and infralimbic cortex of BEP rats compared to No PF controls. Throughout the nucleus accumbens and medial prefrontal cortex, PF induced higher Fos expression in BEP than in BER rats, even after adjusting for differences in PF intake. Differences in the neural activation pattern between BEP and BER rats were more robust in prefrontal cortex than in nucleus accumbens. These data confirm that PF activates brain regions responsible for encoding the incentive salience and hedonic properties of PF, and suggest that binge eating proneness is associated with enhanced responses to PF in brain regions that exert executive control over food reward. Copyright © 2015 Elsevier Inc. All rights reserved.

Differential mesocorticolimbic responses to palatable food in binge eating prone and binge eating resistant female rats

PubMed Central

Sinclair, Elaine B.; Culbert, Kristen M.; Gradl, Dana R.; Richardson, Kimberlei A.; Klump, Kelly L.; Sisk, Cheryl L.

2017-01-01

Binge eating is a key symptom of many eating disorders (e.g. binge eating disorder, bulimia nervosa, anorexia nervosa binge/purge type), yet the neurobiological underpinnings of binge eating are poorly understood. The mesocorticolimbic reward circuit, including the nucleus accumbens and the medial prefrontal cortex, is likely involved because this circuit mediates the hedonic value and incentive salience of palatable foods (PF). Here we tested the hypothesis that higher propensity for binge eating is associated with a heightened response (i.e., Fos induction) of the nucleus accumbens and medial prefrontal cortex to PF, using an animal model that identifies binge eating prone (BEP) and binge eating resistant (BER) rats. Forty adult female Sprague–Dawley rats were given intermittent access to PF (high fat pellets) 3×/week for 3 weeks. Based on a pattern of either consistently high or consistently low PF consumption across these feeding tests, 8 rats met criteria for categorization as BEP, and 11 rats met criteria for categorization as BER. One week after the final feeding test, BEP and BER rats were either exposed to PF in their home cages or were given no PF in their home cages for 1 h prior to perfusion, leading to three experimental groups for the Fos analysis: BEPs given PF, BERs given PF, and a No PF control group. The total number of Fos-immunoreactive (Fos-ir) cells in the nucleus accumbens core and shell, and the cingulate, prelimbic, and infralimbic regions of the medial prefrontal cortex was estimated by stereological analysis. PF induced higher Fos expression in the nucleus accumbens shell and core and in the prelimbic and infralimbic cortex of BEP rats compared to No PF controls. Throughout the nucleus accumbens and medial pre-frontal cortex, PF induced higher Fos expression in BEP than in BER rats, even after adjusting for differences in PF intake. Differences in the neural activation pattern between BEP and BER rats were more robust in prefrontal cortex than in nucleus accumbens. These data confirm that PF activates brain regions responsible for encoding the incentive salience and hedonic properties of PF, and suggest that binge eating proneness is associated with enhanced responses to PF in brain regions that exert executive control over food reward. PMID:26459117

Network Science Center Research Team’s Visit to Kampala, Uganda

DTIC Science & Technology

2013-07-01

Commercial Bank • White Collar Professional • Military Leader 18 | P a g e Network Science Center, West Point www.netscience.usma.edu...people within the social network (Van Der Gaag & Snijders, 2004). 2 | P a g e Network Science Center, West Point www.netscience.usma.edu...University, one of the leading universities in Sub-Saharan Africa. The student body is energetic and tech savvy. 3 | P a g e Network Science

Full colorless transmission of millimeter-wave band gigabit data over WDM-PON using sideband routing

NASA Astrophysics Data System (ADS)

Won, Yong-Yuk; Kim, Hyun-Seung; Son, Yong-Hwan; Han, Sang-Kook

2011-12-01

A new wavelength division multiplexed-radio over fiber (WDM-RoF) access network scheme supporting the simultaneous transmission of a 1.25-Gb/s wired data as well as a 1.25-Gb/s wireless data is proposed in this paper. An optical carrier suppression effect and sideband routing using the multiplexing of arrayed waveguide grating (AWG) with 50-GHz channel spacing are utilized to generate a millimeter wave band carrier. These techniques make the proposed architecture transmit both a wired data and a wireless one at the same time. A reflective semiconductor optical amplifier (RSOA) is employed at both central office and base station so that this architecture is operated colorlessly. Error free transmissions (BER of 10-9) of both downlink and uplink are achieved simultaneously.

A comparative study of three different synthesis routes for hydrophilic fluorophore-doped silica nanoparticles

NASA Astrophysics Data System (ADS)

Shahabi, Shakiba; Treccani, Laura; Rezwan, Kurosch

2016-01-01

The synthesis of fluorophore-doped silica nanoparticles (FDS NPs) with two conventional approaches, Stöber and microemulsion, as well as a novel amino acid-catalyzed seeds regrowth technique (ACSRT) is presented. The efficiency of each applied synthesis route toward incorporation of selected hydrophilic fluorophores, including rhodamine B isothiocyanate and fluorescein isothiocyanate, without and with an amine-containing crosslinker, into silica matrix was systematically studied. Our results clearly highlight the advantages of ACSRT to obtain FDS NPs with a remarkable encapsulation efficiency, high quantum yield, and enhanced stability against bleaching and dye leaking due to efficient embedding of the dyes inside silica network even without the amine-containing silane reagent. Moreover, evaluation of photostability of FDNPs internalized in human bone cells demonstrates the merits of ACSRT.

Analysis of the influence of backscattered optical power over bidirectional PON links

NASA Astrophysics Data System (ADS)

Martínez, J. J.; Garcés, I.; López, A.; Villafranca, A.; Losada, M. A.

2010-05-01

Our aim is to describe the behavior of non-linear scattering effects that arise in standard single mode fiber (SMF), specifically scattering effects that propagate optical power in the reverse direction of the source signal such as Rayleigh Scattering (RS) and Brillouin Scattering (BS). For this purpose, the effects of backscattering phenomena over a bidirectional data transmission in a passive optical network (PON) scheme have been assessed. The impact of these high optical power components over reception at the optical line terminal (OLT) side has been determined when both links use the same wavelength. Bit Error Rate (BER) measurements have been performed with different transmission rates, using several techniques to mitigate the influence of backscattering over the received signal and considering cases with filtered and unfiltered BS.

LiFi: transforming fibre into wireless

NASA Astrophysics Data System (ADS)

Yin, Liang; Islim, Mohamed Sufyan; Haas, Harald

2017-01-01

Light-fidelity (LiFi) uses energy-efficient light-emitting diodes (LEDs) for high-speed wireless communication, and it has a great potential to be integrated with fibre communication for future gigabit networks. However, by making fibre communication wireless, multiuser interference arises. Traditional methods use orthogonal multiple access (OMA) for interference avoidance. In this paper, multiuser interference is exploited with the use of non-orthogonal multiple access (NOMA) relying on successive interference cancellation (SIC). The residual interference due to imperfect SIC in practical scenarios is characterized with a proportional model. Results show that NOMA offers 5 -10 dB gain on the equivalent signal-to-interference-plus-noise ratio (SINR) over OMA. The bit error rate (BER) performance of direct current optical orthogonal frequency division multiplexing (DCO-OFDM) is shown to be significantly improved when SIC is used.

FM and FSK response of tunable two-electrode DFB lasers and their performance with noncoherent detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Willner, A.E.; Kuznetsov, M.; Kaminow, I.P.

1989-12-01

Two-electrode DFB lasers show promise for combining high speed and frequency tunability for FDM-FSK networks. The authors have measured the FM and FSK response of such lasers up to modulation frequencies of {approximately} GHz. Using these lasers in a noncoherent detection system in which a fiber Fabry-Perot tunable optical filter converts an FSK signal into ASK format, the authors demonstrate 10{sup {minus}9} BER up to 1 Gbit/s. Nonuniform FM response and consequent tone broadening of the optical-filtering FSK spectra can lead to system power penalties due to optical-filtering effects. Thus, for a given FM response, they can project the behaviormore » of these lasers in FSK optical systems.« less

A history of the DNA repair and mutagenesis field: The discovery of base excision repair.

PubMed

Friedberg, Errol C

2016-01-01

This article reviews the early history of the discovery of an DNA repair pathway designated as base excision repair (BER), since in contrast to the enzyme-catalyzed removal of damaged bases from DNA as nucleotides [called nucleotide excision repair (NER)], BER involves the removal of damaged or inappropriate bases, such as the presence of uracil instead of thymine, from DNA as free bases. Copyright © 2015. Published by Elsevier B.V.

The Photoviscous Properties of Fluids

DTIC Science & Technology

1942-02-01

and (h) the specific fringe value. The first of these values is de - fined as the velocity gradiont that will produce a rola- tivo retardation of...einer bewegten zähen Flüssigkeit. Ann.-der Phys. (II)t Bd. 151, 1874, p. 154. Kundt i A.: Über die Doppelbrechung des Lichtes in be- wegten...reibenden Flüssigkeiten. Ann. der Phys. (Ill), Bd. 13, 1881, pp. 110-133. deMetz, &.: Über die temporare Doppelbrechung des Lichtes in rotirenden

Mechanism of formation and nanostructure of Stöber silica particles

NASA Astrophysics Data System (ADS)

Masalov, V. M.; Sukhinina, N. S.; Kudrenko, E. A.; Emelchenko, G. A.

2011-07-01

The formation of silica nano- and microparticles has been studied during growth by the modified Stöber-Fink-Bohn (SFB) method. It has been experimentally found that the density and fractal structure of particles vary with size as they grow from 70 to 2200 nm. We propose a model of particle structure which is a dense primary particle core and is composed of concentric secondary particle shells terminating in dense primary particle layers.

Role of DNA base excision repair in the mutability and virulence of Streptococcus mutans

PubMed Central

Gonzalez, Kaisha; Faustoferri, Roberta C.; Quivey, Robert G.

2012-01-01

Summary The oral pathogen, Streptococcus mutans, possesses inducible DNA repair defenses for protection against pH fluctuations and production of reactive oxygen metabolites such as hydrogen peroxide (H2O2), which are present in the oral cavity. DNA base excision repair (BER) has a critical role in genome maintenance by preventing the accumulation of mutations associated with environmental factors and normal products of cellular metabolism. In this study, we examined the consequences of compromising the DNA glycosylases (Fpg and MutY) and endonucleases (Smx and Smn) of the BER pathway and their relative role in adaptation and virulence. Enzymatic characterization of the BER system showed that it protects the organism against the effects of the highly mutagenic lesion, 7,8-dihydro-8-oxo-2’-deoxyguanine (8-oxo-dG). S. mutans strains lacking a functional Fpg, MutY, or Smn showed elevated spontaneous mutation frequencies; and, these mutator phenotypes correlated with the ability of the strains to survive killing by acid and oxidative agents. In addition, in the G. mellonella virulence model, strains of S. mutans deficient in Fpg, MutY and Smn showed increased virulence as compared to the parent strain. Our results suggest that, for S. mutans, mutator phenotypes, due to loss of BER enzymes, may confer an advantage to virulence of the organism. PMID:22651851

EGFR-TKI-induced HSP70 degradation and BER suppression facilitate the occurrence of the EGFR T790 M resistant mutation in lung cancer cells.

PubMed

Cao, Xiang; Zhou, Yi; Sun, Hongfang; Xu, Miao; Bi, Xiaowen; Zhao, Zhihui; Shen, Binghui; Wan, Fengyi; Hong, Zhuan; Lan, Lei; Luo, Lan; Guo, Zhigang; Yin, Zhimin

2018-06-28

Non-small cell lung cancer (NSCLC) patients harboring EGFR-activating mutations initially respond to EGFR tyrosine kinase inhibitors (EGFR-TKIs) and have shown favorable outcomes. However, acquired drug resistance to EGFR-TKIs develops in almost all patients mainly due to the EGFR T790 M mutation. Here, we show that treatment with low-dose EGFR-TKI results in the emergence of the EGFR T790 M mutation and in the reduction of HSP70 protein levels in HCC827 cells. Erlotinib treatment inhibits HSP70 phosphorylation at tyrosine 41 and increases HSP70 ubiquitination, resulting in HSP70 degradation. We show that EGFR-TKI treatment causes increased DNA damage and enhanced gene mutation rates, which are secondary to the EGFR-TKI-induced reduction of HSP70 protein. Importantly, HSP70 overexpression delays the occurrence of Erlotinib-induced EGFR T790 M mutation. We further demonstrate that HSP70 interacts with multiple enzymes in the base excision repair (BER) pathway and promotes not only the efficiency but also the fidelity of BER. Collectively, our findings show that EGFR-TKI treatment facilitates gene mutation and the emergence of EGFR T790 M secondary mutation by the attenuation of BER via induction of HSP70 protein degradation. Copyright © 2018. Published by Elsevier B.V.

Both base excision repair and nucleotide excision repair in humans are influenced by nutritional factors.

PubMed

Brevik, Asgeir; Karlsen, Anette; Azqueta, Amaya; Tirado, Anna Estaban; Blomhoff, Rune; Collins, Andrew

2011-01-01

Lack of reliable assays for DNA repair has largely prevented measurements of DNA repair from being included in human biomonitoring studies. Using newly developed modifications of the comet assay we tested whether a fruit- and antioxidant-rich plant-based intervention could affect base excision repair (BER) and nucleotide excision repair (NER) in a group of 102 male volunteers. BER and NER repair capacities were measured in lymphocytes before and after a dietary intervention lasting 8 weeks. The study had one control group, one group consuming three kiwifruits per day and one group consuming a variety of antioxidant-rich fruits and plant products in addition to their normal diet. DNA strand breaks were reduced following consumption of both kiwifruits (13%, p = 0.05) and antioxidant-rich plant products (20%, p = 0.02). Increased BER (55%, p = 0.01) and reduced NER (-39%, p < 0.01) were observed in the group consuming a wide variety of plant products. Reduced NER was also observed in the kiwifruit group (-38%, p = 0.05), but BER was not affected in this group. Here we have demonstrated that DNA repair is affected by diet and that modified versions of the comet assay can be used to assess activity of different DNA repair pathways in human biomonitoring studies. Copyright © 2010 John Wiley & Sons, Ltd.

The replicative DNA polymerase of herpes simplex virus 1 exhibits apurinic/apyrimidinic and 5′-deoxyribose phosphate lyase activities

PubMed Central

Bogani, Federica; Boehmer, Paul E.

2008-01-01

Base excision repair (BER) is essential for maintaining genome stability both to counter the accumulation of unusual bases and to protect from base loss in the DNA. Herpes simplex virus 1 (HSV-1) is a large dsDNA virus that encodes its own DNA replication machinery, including enzymes involved in nucleotide metabolism. We report on a replicative family B and a herpesvirus-encoded DNA Pol that possesses DNA lyase activity. We have discovered that the catalytic subunit of the HSV-1 DNA polymerase (Pol) (UL30) exhibits apurinic/apyrimidinic (AP) and 5′-deoxyribose phosphate (dRP) lyase activities. These activities are integral to BER and lead to DNA cleavage on the 3′ side of abasic sites and 5′-dRP residues that remain after cleavage by 5′-AP endonuclease. The UL30-catalyzed reaction occurs independently of divalent cation and proceeds via a Schiff base intermediate, indicating that it occurs via a lyase mechanism. Partial proteolysis of the Schiff base shows that the DNA lyase activity resides in the Pol domain of UL30. These observations together with the presence of a virus-encoded uracil DNA glycosylase indicates that HSV-1 has the capacity to perform critical steps in BER. These findings have implications on the role of BER in viral genome maintenance during lytic replication and reactivation from latency. PMID:18695225

NSI customer service representatives and user support office: NASA Science Internet

NASA Technical Reports Server (NTRS)

1991-01-01

The NASA Science Internet, (NSI) was established in 1987 to provide NASA's Offices of Space Science and Applications (OSSA) missions with transparent wide-area data connectivity to NASA's researchers, computational resources, and databases. The NSI Office at NASA/Ames Research Center has the lead responsibility for implementing a total, open networking program to serve the OSSA community. NSI is a full-service communications provider whose services include science network planning, network engineering, applications development, network operations, and network information center/user support services. NSI's mission is to provide reliable high-speed communications to the NASA science community. To this end, the NSI Office manages and operates the NASA Science Internet, a multiprotocol network currently supporting both DECnet and TCP/IP protocols. NSI utilizes state-of-the-art network technology to meet its customers' requirements. THe NASA Science Internet interconnects with other national networks including the National Science Foundation's NSFNET, the Department of Energy's ESnet, and the Department of Defense's MILNET. NSI also has international connections to Japan, Australia, New Zealand, Chile, and several European countries. NSI cooperates with other government agencies as well as academic and commercial organizations to implement networking technologies which foster interoperability, improve reliability and performance, increase security and control, and expedite migration to the OSI protocols.

Performance Enhancement of Multichannel Gigabit Rate Bidirectional WDM-PON Using RSOA and Optimized Modulation Format

NASA Astrophysics Data System (ADS)

Parkash, Sooraj; Sharma, Anurag; Singh, Harsukhpreet

2016-09-01

This paper successfully demonstrates bidirectional wavelength division multiplexing passive optical network (WDM-PON) system for 32 channels, 0.8 nm (100 GHz) channels spacing with 3.5 GHz filter bandwidth. The system delivers 160 GB/s data rate and 80 GB/s data rate in downstream and upstream, respectively. The optical source for downstream data and upstream data is mode-locked laser at central office and reflective semiconductor optical amplifier (RSOA) at optical network unit. The maximum reach of designed system is 50 km without using any dispersion compensation scheme. This paper comprises comparison of series of modulation format in downstream and upstream such as SOLITON, NRZ, RZ, MANCHESTER, CSRZ and CRZ-DPSK and optimization of the performance of designed system. It has been observed that CRZ-DPSK/NRZ gives best performance in downstream and upstream transmission for designed system. The simulation work report of minimum BER is e-13 for CRZ-DPSK in downstream and e-16 for NRZ in upstream transmission in case of 32-channel bidirectional WDM-PON.

Remote Observing with the Keck Telescope Using the ACTS Satellite

NASA Technical Reports Server (NTRS)

Cohen, Judy; Shopbell, Patrick; Bergman, Larry

1998-01-01

As a technical demonstration project for the NASA Advanced Communications Technology Satellite (ACTS), we have implemented remote observing on the 10-meter Keck II telescope on Mauna Kea in Hawaii from the California Institute of Technology campus in Pasadena. The data connection consists of optical fiber networks in Hawaii and California, connecting the end-points to high data rate (HDR) ACTS satellite antennae at JPL in Pasadena and at the Tripler Army Medical Center in Honolulu. The terrestrial fiber networks run the asynchronous transfer mode (ATM) protocol at DS-3 (45 Mbit/sec) speeds, providing ample bandwidth to enable remote observing with a software environment identical to that used for on-site observing in Hawaii. This experiment has explored the data requirements of remote observing with a modern research telescope and large-format detector arrays. While the maximum burst data rates are lower than those required for many other applications (e.g., HDTV), the network reliability and data integrity requirements are critical. As we show in this report, the former issue particularly may be the greatest challenge for satellite networks for this class of application. We have also experimented with the portability of standard TCP/IP applications to satellite networks, demonstrating the need for alternative TCP congestion algorithms and minimization of bit error rates (BER). Reliability issues aside, we have demonstrated that true remote observing over high-speed networks provides several important advantages over standard observing paradigms. Technical advantages of the high-speed network access include more rapid download of data to a user's home institution and the opportunity for alternative communication facilities between members of an observing team, such as audio- and videoconferencing.

New paradigms in the repair of oxidative damage in human genome: mechanisms ensuring repair of mutagenic base lesions during replication and involvement of accessory proteins.

PubMed

Dutta, Arijit; Yang, Chunying; Sengupta, Shiladitya; Mitra, Sankar; Hegde, Muralidhar L

2015-05-01

Oxidized bases in the mammalian genome, which are invariably mutagenic due to their mispairing property, are continuously induced by endogenous reactive oxygen species and more abundantly after oxidative stress. Unlike bulky base adducts induced by UV and other environmental mutagens in the genome that block replicative DNA polymerases, oxidatively damaged bases such as 5-hydroxyuracil, produced by oxidative deamination of cytosine in the template strand, do not block replicative polymerases and thus need to be repaired prior to replication to prevent mutation. Following up our earlier studies, which showed that the Nei endonuclease VIII like 1 (NEIL1) DNA glycosylase, one of the five base excision repair (BER)-initiating enzymes in mammalian cells, has enhanced expression during the S-phase and higher affinity for replication fork-mimicking single-stranded (ss) DNA substrates, we recently provided direct experimental evidence for NEIL1's role in replicating template strand repair. The key requirement for this event, which we named as the 'cow-catcher' mechanism of pre-replicative BER, is NEIL1's non-productive binding (substrate binding without product formation) to the lesion base in ss DNA template to stall DNA synthesis, causing fork regression. Repair of the lesion in reannealed duplex is then carried out by NEIL1 in association with the DNA replication proteins. NEIL1 (and other BER-initiating enzymes) also interact with several accessory and non-canonical proteins including the heterogeneous nuclear ribonucleoprotein U and Y-box-binding protein 1 as well as high mobility group box 1 protein, whose precise roles in BER are still obscure. In this review, we have discussed the recent advances in our understanding of oxidative genome damage repair pathways with particular focus on the pre-replicative template strand repair and the role of scaffold factors like X-ray repairs cross-complementing protein 1 and poly (ADP-ribose) polymerase 1 and other accessory proteins guiding distinct BER sub-pathways.

Cepstral domain modification of audio signals for data embedding: preliminary results

NASA Astrophysics Data System (ADS)

Gopalan, Kaliappan

2004-06-01

A method of embedding data in an audio signal using cepstral domain modification is described. Based on successful embedding in the spectral points of perceptually masked regions in each frame of speech, first the technique was extended to embedding in the log spectral domain. This extension resulted at approximately 62 bits /s of embedding with less than 2 percent of bit error rate (BER) for a clean cover speech (from the TIMIT database), and about 2.5 percent for a noisy speech (from an air traffic controller database), when all frames - including silence and transition between voiced and unvoiced segments - were used. Bit error rate increased significantly when the log spectrum in the vicinity of a formant was modified. In the next procedure, embedding by altering the mean cepstral values of two ranges of indices was studied. Tests on both a noisy utterance and a clean utterance indicated barely noticeable perceptual change in speech quality when lower range of cepstral indices - corresponding to vocal tract region - was modified in accordance with data. With an embedding capacity of approximately 62 bits/s - using one bit per each frame regardless of frame energy or type of speech - initial results showed a BER of less than 1.5 percent for a payload capacity of 208 embedded bits using the clean cover speech. BER of less than 1.3 percent resulted for the noisy host with a capacity was 316 bits. When the cepstrum was modified in the region of excitation, BER increased to over 10 percent. With quantization causing no significant problem, the technique warrants further studies with different cepstral ranges and sizes. Pitch-synchronous cepstrum modification, for example, may be more robust to attacks. In addition, cepstrum modification in regions of speech that are perceptually masked - analogous to embedding in frequency masked regions - may yield imperceptible stego audio with low BER.

Network Science Center Research Team’s Visit to Kampala, Uganda

DTIC Science & Technology

2013-04-15

Leader • Someone in Social Network • Commercial Bank • White Collar Professional • Military Leader 16 | P a g e Network Science Center, West Point www.netscience.usma.edu 845.938.0804 ...regions, where the Lord’s Resistance Army 2 | P a g e Network Science Center, West Point www.netscience.usma.edu 845.938.0804 (LRA), a militant...Non-Governmental Organizations. Teddy Ruge Kanjokya Road in Kamwokya 3 | P a g e Network Science Center, West Point

The Joint Chiefs of Staff and National Policy 1951-1953, Volume III, The Korean War: Part Two,

DTIC Science & Technology

1998-01-01

equally by the Commander in Chief, United Nations Com- mand, and the Commander in Chief of the Communist forces in Korea, in num - bers sufficient to...reached on a limiting num - ber that would satisfy General Ridgway’s maximum rotation requirements. The 53 JCS and National Policy important point... sanguine about the success of these efforts.79 The fate of civilian captives of non-ROK origin—missionaries, personnel of neutral embassies, press

Correlation Between Analog Noise Measurements and the Expected Bit Error Rate of a Digital Signal Propagating Through Passive Components

NASA Technical Reports Server (NTRS)

Warner, Joseph D.; Theofylaktos, Onoufrios

2012-01-01

A method of determining the bit error rate (BER) of a digital circuit from the measurement of the analog S-parameters of the circuit has been developed. The method is based on the measurement of the noise and the standard deviation of the noise in the S-parameters. Once the standard deviation and the mean of the S-parameters are known, the BER of the circuit can be calculated using the normal Gaussian function.

Southeast Asia Report

DTIC Science & Technology

1987-04-16

as a political party in ScptcnW ber 1986 during a Metro Manila con- vention held by Joma Sison and Ber- nabe Buscayno. In a decision denying the...at plOO. The talks have become even more complicated because of two recent developments. UCPB Chairman Ramon Sy , recently appointed to the SMC board...for probability The Green Star or Yellow Star movement could be: !. A once-active „oop of T roraes » -JS^S-.’^fS.^Lfl S* relfstar which L th4 sy -ol

Research on parallel combinatory spread spectrum communication system with double information matching

NASA Astrophysics Data System (ADS)

Xue, Wei; Wang, Qi; Wang, Tianyu

2018-04-01

This paper presents an improved parallel combinatory spread spectrum (PC/SS) communication system with the method of double information matching (DIM). Compared with conventional PC/SS system, the new model inherits the advantage of high transmission speed, large information capacity and high security. Besides, the problem traditional system will face is the high bit error rate (BER) and since its data-sequence mapping algorithm. Hence the new model presented shows lower BER and higher efficiency by its optimization of mapping algorithm.

Optimization of lens layout for THz signal free-space delivery

NASA Astrophysics Data System (ADS)

Yu, Jimmy; Zhou, Wen

2018-03-01

We investigate how to extend the air-space distance for Terahertz (THz) signal by using optimized lens layout. After a delivery over 129.6 cm air-space we realize the BER of 10 Gb/s QPSK signal at 450 GHz smaller than 1 ×10-4 with this optimized lens layout. If only two lenses are employed, the BER is higher than forward error correction (FEC) threshold at the input power of 15 dBm into the photodiode.

Multiple Beam Torus Antenna Study. Volume 2

DTIC Science & Technology

1977-03-01

matezal around the feed horn aperture yields 60Amin - (2 - 4 OHP (5-36) The minimum beam spacing for the front-fed MBTA as a function of D/X with -10- and...members were included. Secondly, the structural stresses on mem - bers at the survival loads were checked. Any member exceeding the yield stress limit...or buckling stress criteria was stiffened accordingly. The yield stress for the backup truss and support mem - bers was azsnad to be 36,000 psi for A36

Fusion Energy Sciences Network Requirements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dart, Eli; Tierney, Brian

2012-09-26

The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy Office of Science, the single largest supporter of basic research in the physical sciences in the United States. In support of the Office of Science programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 25 years. In December 2011, ESnet and the Office of Fusion Energy Sciences (FES), of the DOE Officemore » of Science (SC), organized a workshop to characterize the networking requirements of the programs funded by FES. The requirements identified at the workshop are summarized in the Findings section, and are described in more detail in the body of the report.« less

Motivation for palatable food despite consequences in an animal model of binge eating.

PubMed

Oswald, Kimberly D; Murdaugh, Donna L; King, Vinetra L; Boggiano, Mary M

2011-04-01

Binge eating involves an abnormal motivation for highly palatable food in that these foods are repeatedly consumed despite their binge-triggering effects and life-affecting consequences associated with binge eating. We determined if rats identified as binge-eating prone (BEP) similarly display abnormal motivation for palatable food. Food-sated BEP and binge-eating resistant (BER) rats were given voluntary access to palatable food paired with increasing intensity of footshock. Later, they were exposed to a period of cyclic caloric restriction-refeeding. BEPs consumed significantly more and tolerated higher levels of footshock for palatable food than BERs. Cyclic restriction-refeeding increased BERs' tolerance of shock for palatable food. Previously observed parallels of the rat BEP model to human binge eating can now be extended to include an abnormal motivation for palatable food. This model should prove useful in identifying specific genes that interact with the nutritional environment to mediate binge eating and may point to novel physiological targets to treat compulsive overeating. Copyright © 2010 Wiley Periodicals, Inc.

A two layer chaotic encryption scheme of secure image transmission for DCT precoded OFDM-VLC transmission

NASA Astrophysics Data System (ADS)

Wang, Zhongpeng; Chen, Fangni; Qiu, Weiwei; Chen, Shoufa; Ren, Dongxiao

2018-03-01

In this paper, a two-layer image encryption scheme for a discrete cosine transform (DCT) precoded orthogonal frequency division multiplexing (OFDM) visible light communication (VLC) system is proposed. Firstly, in the proposed scheme the transmitted image is first encrypted by a chaos scrambling sequence,which is generated from the hybrid 4-D hyper- and Arnold map in the upper-layer. After that, the encrypted image is converted into digital QAM modulation signal, which is re-encrypted by chaos scrambling sequence based on Arnold map in physical layer to further enhance the security of the transmitted image. Moreover, DCT precoding is employed to improve BER performance of the proposed system and reduce the PAPR of OFDM signal. The BER and PAPR performances of the proposed system are evaluated by simulation experiments. The experiment results show that the proposed two-layer chaos scrambling schemes achieve image secure transmission for image-based OFDM VLC. Furthermore, DCT precoding can reduce the PAPR and improve the BER performance of OFDM-based VLC.

Motivation for Palatable Food Despite Consequences in an Animal Model of Binge-Eating

PubMed Central

Oswald, Kimberly D.; Murdaugh, Donna L.; King, Vinetra L.; Boggiano, Mary M.

2010-01-01

Objective Binge-eating involves an abnormal motivation for highly palatable food in that these foods are repeatedly consumed despite their binge-triggering effects and life-affecting consequences associated with binge-eating. We determined if rats identified as binge-eating prone (BEP) similarly display abnormal motivation for palatable food. Method Food-sated BEP and binge-eating resistant (BER) rats were given voluntary access to palatable food paired with increasing intensity of footshock. Later, they were exposed to a period of cyclic caloric restriction-refeeding. Results BEPs consumed significantly more and tolerated higher levels of footshock for palatable food than BERs. Cyclic restriction-refeeding increased BERs' tolerance of shock for palatable food. Discussion Previously observed parallels of the rat BEP model to human binge-eating can now be extended to include an abnormal motivation for palatable food. This model should prove useful in identifying specific genes that interact with the nutritional environment to mediate binge-eating and may point to novel physiological targets to treat compulsive overeating. PMID:20186718

Effects of fog on the bit-error rate of a free-space laser communication system.

PubMed

Strickland, B R; Lavan, M J; Woodbridge, E; Chan, V

1999-01-20

Free-space laser communication (lasercom) systems are subject to performance degradation when heavy fog or smoke obscures the line of sight. The bit-error rate (BER) of a high-bandwidth (570 Mbits/s) lasercom system was correlated with the atmospheric transmission over a folded path of 2.4 km. BER's of 10(-7) were observed when the atmospheric transmission was as low as 0.25%, whereas BER's of less than 10(-10) were observed when the transmission was above 2.5%. System performance was approximately 10 dB less than calculated, with the discrepancy attributed to scintillation, multiple scattering, and absorption. Peak power of the 810-nm communications laser was 186 mW, and the beam divergence was purposely degraded to 830 murad. These results were achieved without the use of error correction schemes or active tracking. An optimized system with narrower beam divergence and active tracking could be expected to yield significantly better performance.

Performance analysis of visible light communication using the STBC-OFDM technique for intelligent transportation systems

NASA Astrophysics Data System (ADS)

Li, Changping; Yi, Ying; Lee, Kyujin; Lee, Kyesan

2014-08-01

Visible light communication (VLC) applied in an intelligent transportation system (ITS) has attracted growing attentions, but it also faces challenges, for example deep path loss and optical multi-path dispersion. In this work, we modelled an actual outdoor optical channel as a Rician channel and further proposed space-time block coding (STBC) orthogonal frequency-division multiplexing (OFDM) technology to reduce the influence of severe optical multi-path dispersion associated with such a mock channel for achieving the effective BER of 10-6 even at a low signal-to-noise ratio (SNR). In this case, the optical signals transmission distance can be extended as long as possible. Through the simulation results of STBC-OFDM and single-input-single-output (SISO) counterparts in bit error rate (BER) performance comparison, we can distinctly observe that the VLC-ITS system using STBC-OFDM technique can obtain a strongly improved BER performance due to multi-path dispersion alleviation.

Crosstalk between MSH2-MSH3 and polβ promotes trinucleotide repeat expansion during base excision repair.

PubMed

Lai, Yanhao; Budworth, Helen; Beaver, Jill M; Chan, Nelson L S; Zhang, Zunzhen; McMurray, Cynthia T; Liu, Yuan

2016-08-22

Studies in knockout mice provide evidence that MSH2-MSH3 and the BER machinery promote trinucleotide repeat (TNR) expansion, yet how these two different repair pathways cause the mutation is unknown. Here we report the first molecular crosstalk mechanism, in which MSH2-MSH3 is used as a component of the BER machinery to cause expansion. On its own, pol β fails to copy TNRs during DNA synthesis, and bypasses them on the template strand to cause deletion. Remarkably, MSH2-MSH3 not only stimulates pol β to copy through the repeats but also enhances formation of the flap precursor for expansion. Our results provide direct evidence that MMR and BER, operating together, form a novel hybrid pathway that changes the outcome of TNR instability from deletion to expansion during the removal of oxidized bases. We propose that cells implement crosstalk strategies and share machinery when a canonical pathway is ineffective in removing a difficult lesion.

Crosstalk between MSH2–MSH3 and polβ promotes trinucleotide repeat expansion during base excision repair

PubMed Central

Lai, Yanhao; Budworth, Helen; Beaver, Jill M.; Chan, Nelson L. S.; Zhang, Zunzhen; McMurray, Cynthia T.; Liu, Yuan

2016-01-01

Studies in knockout mice provide evidence that MSH2–MSH3 and the BER machinery promote trinucleotide repeat (TNR) expansion, yet how these two different repair pathways cause the mutation is unknown. Here we report the first molecular crosstalk mechanism, in which MSH2–MSH3 is used as a component of the BER machinery to cause expansion. On its own, pol β fails to copy TNRs during DNA synthesis, and bypasses them on the template strand to cause deletion. Remarkably, MSH2–MSH3 not only stimulates pol β to copy through the repeats but also enhances formation of the flap precursor for expansion. Our results provide direct evidence that MMR and BER, operating together, form a novel hybrid pathway that changes the outcome of TNR instability from deletion to expansion during the removal of oxidized bases. We propose that cells implement crosstalk strategies and share machinery when a canonical pathway is ineffective in removing a difficult lesion. PMID:27546332

Performance analysis of coherent free space optical communications with sequential pyramid wavefront sensor

NASA Astrophysics Data System (ADS)

Liu, Wei; Yao, Kainan; Chen, Lu; Huang, Danian; Cao, Jingtai; Gu, Haijun

2018-03-01

Based-on the previous study on the theory of the sequential pyramid wavefront sensor (SPWFS), in this paper, the SPWFS is first applied to the coherent free space optical communications (FSOC) with more flexible spatial resolution and higher sensitivity than the Shack-Hartmann wavefront sensor, and with higher uniformity of intensity distribution and much simpler than the pyramid wavefront sensor. Then, the mixing efficiency (ME) and the bit error rate (BER) of the coherent FSOC are analyzed during the aberrations correction through numerical simulation with binary phase shift keying (BPSK) modulation. Finally, an experimental AO system based-on SPWFS is setup, and the experimental data is used to analyze the ME and BER of homodyne detection with BPSK modulation. The results show that the AO system based-on SPWFS can increase ME and decrease BER effectively. The conclusions of this paper provide a new method of wavefront sensing for designing the AO system for a coherent FSOC system.

Dependence of the bit error rate on the signal power and length of a single-channel coherent single-span communication line (100 Gbit s{sup -1}) with polarisation division multiplexing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gurkin, N V; Konyshev, V A; Novikov, A G

2015-01-31

We have studied experimentally and using numerical simulations and a phenomenological analytical model the dependences of the bit error rate (BER) on the signal power and length of a coherent single-span communication line with transponders employing polarisation division multiplexing and four-level phase modulation (100 Gbit s{sup -1} DP-QPSK format). In comparing the data of the experiment, numerical simulations and theoretical analysis, we have found two optimal powers: the power at which the BER is minimal and the power at which the fade margin in the line is maximal. We have derived and analysed the dependences of the BER on themore » optical signal power at the fibre line input and the dependence of the admissible input signal power range for implementation of the communication lines with a length from 30 – 50 km up to a maximum length of 250 km. (optical transmission of information)« less

Direct bit detection receiver noise performance analysis for 32-PSK and 64-PSK modulated signals

NASA Astrophysics Data System (ADS)

Ahmed, Iftikhar

1987-12-01

Simple two channel receivers for 32-PSK and 64-PSK modulated signals have been proposed which allow digital data (namely bits), to be recovered directly instead of the traditional approach of symbol detection followed by symbol to bit mappings. This allows for binary rather than M-ary receiver decisions, reduces the amount of signal processing operations and permits parallel recovery of the bits. The noise performance of these receivers quantified by the Bit Error Rate (BER) assuming an Additive White Gaussian Noise interference model is evaluated as a function of Eb/No, the signal to noise ratio, and transmitted phase angles of the signals. The performance results of the direct bit detection receivers (DBDR) when compared to that of convectional phase measurement receivers demonstrate that DBDR's are optimum in BER sense. The simplicity of the receiver implementations and the BER of the delivered data make DBDR's attractive for high speed, spectrally efficient digital communication systems.

Networked Improvement Communities: The Discipline of Improvement Science Meets the Power of Networks

ERIC Educational Resources Information Center

LeMahieu, Paul G.; Grunow, Alicia; Baker, Laura; Nordstrum, Lee E.; Gomez, Louis M.

2017-01-01

Purpose: The purpose of this paper is to delineate an approach to quality assurance in education called networked improvement communities (NICs) that focused on integrating the methodologies of improvement science with few of the networks. Quality improvement, the science and practice of continuously improving programs, practices, processes,…

Network Science Center Research Team’s Visit to Kampala, Uganda

DTIC Science & Technology

2013-07-01

Religious Leader • Someone in Social Network • Commercial Bank • White Collar Professional • Military Leader 18 | P a g e Network Science...relationship between the person under analysis and the people within the social network (Van Der Gaag & Snijders, 2004). 2 | P a g e Network...energetic and tech savvy. 3 | P a g e Network Science Center, West Point www.netscience.usma.edu 845.938.0804 Our team’s next data collection effort

"Getting Practical" and the National Network of Science Learning Centres

ERIC Educational Resources Information Center

Chapman, Georgina; Langley, Mark; Skilling, Gus; Walker, John

2011-01-01

The national network of Science Learning Centres is a co-ordinating partner in the Getting Practical--Improving Practical Work in Science programme. The principle of training provision for the "Getting Practical" programme is a cascade model. Regional trainers employed by the national network of Science Learning Centres trained the cohort of local…

75 FR 65363 - Basic Behavioral and Social Science Opportunity Network (OppNet)

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-22

... public meeting to promote and publicize the Basic Behavioral and Social Science Opportunity Network (Opp... . Background: The Basic Behavioral and Social Science Opportunity Network (OppNet) is a trans-NIH initiative to expand the agency's funding of basic behavioral and social sciences research (b-BSSR). OppNet prioritizes...

Analysis of repair and PCNA complex formation induced by ionizing radiation in human fibroblast cell lines.

PubMed

Karmakar, P; Balajee, A S; Natarajan, A T

2001-05-01

Proliferating cell nuclear antigen (PCNA), an auxiliary factor for DNA polymerase delta and epsilon, is involved in both DNA replication and repair. Previous studies in vitro have demonstrated the requirement of PCNA in the resynthesis step of nucleotide excision repair (NER) and base excision repair (BER). Using a native chromatin template isolated under near physiological conditions, we have analysed the involvement of PCNA in the BER pathway in different NER defective human cell lines. The repair sites and PCNA were visualized by indirect immunolabelling followed by fluorescence microscopy. The results indicate that exposure to X-rays triggers the induction of PCNA in all the three human fibroblast cell lines studied, namely normal, xeroderma pigmentosum group A (XP-A) and Cockayne syndrome group B (CS-B). In all the cell lines, induction of PCNA and repair patches occurred in a dose- and time-dependent fashion. Induction of repair patches in NER-deficient XP-A cells suggests that the X-ray-induced lesions are largely repaired via the BER pathway involving PCNA as one of the key components of this pathway. X-ray-induced repair synthesis was greatly inhibited by treatment of cells with DNA polymerase inhibitors aphidicolin and cytosine arabinoside. Interestingly, inhibition of repair resynthesis did not affect the intensity of PCNA staining in X-irradiated cells indicating that the PCNA may be required for the BER pathway at a step preceding the resynthesis step.

Abscisic acid triggers whole-plant and fruit-specific mechanisms to increase fruit calcium uptake and prevent blossom end rot development in tomato fruit.

PubMed

de Freitas, Sergio Tonetto; Shackel, Kenneth A; Mitcham, Elizabeth J

2011-05-01

Calcium (Ca) uptake into fruit and leaves is dependent on xylemic water movement, and hence presumably driven by transpiration and growth. High leaf transpiration is thought to restrict Ca movement to low-transpiring tomato fruit, which may increase fruit susceptibility to the Ca-deficiency disorder, blossom end rot (BER). The objective of this study was to analyse the effect of reduced leaf transpiration in abscisic acid (ABA)-treated plants on fruit and leaf Ca uptake and BER development. Tomato cultivars Ace 55 (Vf) and AB2 were grown in a greenhouse environment under Ca-deficit conditions and plants were treated weekly after pollination with water (control) or 500 mg l(-1) ABA. BER incidence was completely prevented in the ABA-treated plants and reached values of 30-45% in the water-treated controls. ABA-treated plants had higher stem water potential, lower leaf stomatal conductance, and lower whole-plant water loss than water-treated plants. ABA treatment increased total tissue and apoplastic water-soluble Ca concentrations in the fruit, and decreased Ca concentrations in leaves. In ABA-treated plants, fruit had a higher number of Safranin-O-stained xylem vessels at early stages of growth and development. ABA treatment reduced the phloem/xylem ratio of fruit sap uptake. The results indicate that ABA prevents BER development by increasing fruit Ca uptake, possibly by a combination of whole-plant and fruit-specific mechanisms.

Effect of human umbilical cord mesenchymal stem cells transplantation on nerve fibers of a rat model of endometriosis.

PubMed

Chen, Yan; Li, Dong; Zhang, Zhe; Takushige, Natsuko; Kong, Bei-Hua; Wang, Guo-Yun

2015-01-01

Endometriosis is a common, benign, oestrogen-dependent, chronic gynaecological disorder associated with pelvic pain and infertility. Some researchers have identified nerve fibers in endometriotic lesions in women with endometriosis. Mesenchymal stem cells (MSCs) have attracted interest for their possible use for both cell and gene therapies because of their capacity for self-renewal and multipotentiality of differentiation. We investigated how human umbilical cord-MSCs (hUC-MSCs) could affect nerve fibers density in endometriosis. In this experimental study, hUC-MSCs were isolated from fresh human umbilical cord, characterized by flow cytometry, and then transplanted into surgically induced endometriosis in a rat model. Ectopic endometrial implants were collected four weeks later. The specimens were sectioned and stained immunohistochemically with antibodies against neurofilament (NF), nerve growth factor (NGF), NGF receptor p75 (NGFRp75), tyrosine kinase receptor-A (Trk-A), calcitonin gene-related peptide (CGRP) and substance P (SP) to compare the presence of different types of nerve fibers between the treatment group with the transplantation of hUC-MSCs and the control group without the transplantation of hUC-MSCs. There were significantly less nerve fibers stained with specific markers we used in the treatment group than in the control group (p<0.05). MSC from human umbilical cord reduced nerve fiber density in the treatment group with the transplantation of hUC-MSCs.

Ntg1p, the base excision repair protein, generates mutagenic intermediates in yeast mitochondrial DNA.

PubMed

Phadnis, Naina; Mehta, Reema; Meednu, Nida; Sia, Elaine A

2006-07-13

Mitochondrial DNA is predicted to be highly prone to oxidative damage due to its proximity to free radicals generated by oxidative phosphorylation. Base excision repair (BER) is the primary repair pathway responsible for repairing oxidative damage in nuclear and mitochondrial genomes. In yeast mitochondria, three N-glycosylases have been identified so far, Ntg1p, Ogg1p and Ung1p. Ntg1p, a broad specificity N-glycosylase, takes part in catalyzing the first step of BER that involves the removal of the damaged base. In this study, we examined the role of Ntg1p in maintaining yeast mitochondrial genome integrity. Using genetic reporters and assays to assess mitochondrial mutations, we found that loss of Ntg1p suppresses mitochondrial point mutation rates, frameshifts and recombination rates. We also observed a suppression of respiration loss in the ntg1-Delta cells in response to ultraviolet light exposure implying an overlap between BER and UV-induced damage in the yeast mitochondrial compartment. Over-expression of the BER AP endonuclease, Apn1p, did not significantly affect the mitochondrial mutation rate in the presence of Ntg1p, whereas Apn1p over-expression in an ntg1-Delta background increased the frequency of mitochondrial mutations. In addition, loss of Apn1p also suppressed mitochondrial point mutations. Our work suggests that both Ntg1p and Apn1p generate mutagenic intermediates in the yeast mitochondrial genome.

DIFFERENTIAL ROLE OF BASE EXCISION REPAIR PROTEINS IN MEDIATING CISPLATIN CYTOTOXICITY

PubMed Central

Sawant, Akshada; Floyd, Ashley M.; Dangeti, Mohan; Lei, Wen; Sobol, Robert W.; Patrick, Steve M.

2017-01-01

Interstrand crosslinks (ICLs) are covalent lesions formed by cisplatin. The mechanism for the processing and removal of ICLs by DNA repair proteins involves nucleotide excision repair (NER), homologous recombination (HR) and fanconi anemia (FA) pathways. In this report, we monitored the processing of a flanking uracil adjacent to a cisplatin ICL by the proteins involved in the base excision repair (BER) pathway. Using a combination of extracts, purified proteins, inhibitors, functional assays and cell culture studies, we determined the specific BER proteins required for processing a DNA substrate with a uracil adjacent to a cisplatin ICL. Uracil DNA glycosylase (UNG) is the primary glycosylase responsible for the removal of uracils adjacent to cisplatin ICLs, whereas other uracil glycosylases can process uracils in the context of undamaged DNA. Repair of the uracil adjacent to cisplatin ICLs proceeds through the classical BER pathway, highlighting the importance of specific proteins in this redundant pathway. Removal of uracil is followed by the generation of an abasic site and subsequent cleavage by AP endonuclease 1 (APE1). Inhibition of either the repair or redox domain of APE1 gives rise to cisplatin resistance. Inhibition of the lyase domain of Polymerase β (Polβ) does not influence cisplatin cytotoxicity. In addition, lack of XRCC1 leads to increased DNA damage and results in increased cisplatin cytotoxicity. Our results indicate that BER activation at cisplatin ICLs influences crosslink repair and modulates cisplatin cytotoxicity via specific UNG, APE1 and Polβ polymerase functions. PMID:28110804

Biological mechanisms beyond network analysis via mathematical modeling. Comment on "Network science of biological systems at different scales: A review" by Marko Gosak et al.

NASA Astrophysics Data System (ADS)

Pedersen, Morten Gram

2018-03-01

Methods from network theory are increasingly used in research spanning from engineering and computer science to psychology and the social sciences. In this issue, Gosak et al. [1] provide a thorough review of network science applications to biological systems ranging from the subcellular world via neuroscience to ecosystems, with special attention to the insulin-secreting beta-cells in pancreatic islets.

The ASE Improving Practical Work in Triple Science Learning Skills Network

ERIC Educational Resources Information Center

Barber, Paul; Chapman, Georgina; Ellis-Sackey, Cecilia; Grainger, Beth; Jones, Steve

2011-01-01

In July 2010, the Association for Science Education won a bid to run a "Sharing innovation network" for the Triple Science Support Programme, which is delivered by the Learning Skills Network on behalf of the Department for Education. The network involves schools from the London boroughs of Tower Hamlets and Greenwich. In this article,…

Go Ask Alice: Uncovering the Role of a University Partner in an Informal Science Curriculum Support Network

ERIC Educational Resources Information Center

Baker-Doyle, Kira J.

2013-01-01

This article describes a study from the Linking Instructors Networks of Knowledge in Science Education project, which aims to examine the informal science curriculum support networks of teachers in a school-university curriculum reform partnership. We used social network analysis and qualitative methods to reveal characteristics of the informal…

Secret Key Generation via a Modified Quantum Secret Sharing Protocol

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith IV, Amos M; Evans, Philip G; Lawrie, Benjamin J

We present and experimentally show a novel protocol for distributing secret information between two and only two parties in a N-party single-qubit Quantum Secret Sharing (QSS) system. We demonstrate this new algorithm with N = 3 active parties over 6km of telecom. ber. Our experimental device is based on the Clavis2 Quantum Key Distribution (QKD) system built by ID Quantique but is generalizable to any implementation. We show that any two out of the N parties can build secret keys based on partial information from each other and with collaboration from the remaining N > 2 parties. This algorithm allowsmore » for the creation of two-party secret keys were standard QSS does not and signicantly reduces the number of resources needed to implement QKD on a highly connected network such as the electrical grid.« less

1.25-3.125 Gb/s per user PON with RSOA as phase modulator for statistical wavelength ONU

NASA Astrophysics Data System (ADS)

Chu, Guang Yong; Polo, Victor; Lerín, Adolfo; Tabares, Jeison; Cano, Iván N.; Prat, Josep

2015-12-01

We report a new scheme to support, cost efficiently, ultra-dense wavelength division multiplexing (UDWDM) for optical access networks. As validating experiment, we apply phase modulation of a reflective semiconductor optical amplifier (RSOA) at the ONU with a single DFB, and simplified coherent receiver at OLT for upstream. We extend the limited 3-dB modulation bandwidth of available uncooled To-can packaged RSOA (~400 MHz) and operate it at 3.125 Gb/s with the optimal performance for phase modulation using small and large signal measurement characteristics. The optimal condition is selected at input power of 0 dBm, with 70 mA bias condition. The sensitivities at 3.125 Gb/s (at BER=10-3) for heterodyne and intradyne detection reach -34.3 dBm and -38.8 dBm, respectively.

Quantum red-green-blue image steganography

NASA Astrophysics Data System (ADS)

Heidari, Shahrokh; Pourarian, Mohammad Rasoul; Gheibi, Reza; Naseri, Mosayeb; Houshmand, Monireh

One of the most considering matters in the field of quantum information processing is quantum data hiding including quantum steganography and quantum watermarking. This field is an efficient tool for protecting any kind of digital data. In this paper, three quantum color images steganography algorithms are investigated based on Least Significant Bit (LSB). The first algorithm employs only one of the image’s channels to cover secret data. The second procedure is based on LSB XORing technique, and the last algorithm utilizes two channels to cover the color image for hiding secret quantum data. The performances of the proposed schemes are analyzed by using software simulations in MATLAB environment. The analysis of PSNR, BER and Histogram graphs indicate that the presented schemes exhibit acceptable performances and also theoretical analysis demonstrates that the networks complexity of the approaches scales squarely.

An Analytical Approach for Performance Enhancement of FSO Communication System Using Array of Receivers in Adverse Weather Conditions

NASA Astrophysics Data System (ADS)

Nagpal, Shaina; Gupta, Amit

2017-08-01

Free Space Optics (FSO) link exploits the tremendous network capacity and is capable of offering wireless communications similar to communications through optical fibres. However, FSO link is extremely weather dependent and the major effect on FSO links is due to adverse weather conditions like fog and snow. In this paper, an FSO link is designed using an array of receivers. The disparity of the link for very high attenuation conditions due to fog and snow is analysed using aperture averaging technique. Further effect of aperture averaging technique is investigated by comparing the systems using aperture averaging technique with systems not using aperture averaging technique. The performance of proposed model of FSO link has been evaluated in terms of Q factor, bit error rate (BER) and eye diagram.

All-optical two-way relaying free-space optical communications for HAP-based broadband backhaul networks

NASA Astrophysics Data System (ADS)

Vu, Minh Q.; Nguyen, Nga T. T.; Pham, Hien T. T.; Dang, Ngoc T.

2018-03-01

High-altitude platforms (HAPs) are flexible, non-pollutant and cost-effective infrastructures compared to satellite or old terrestrial systems. They are being researched and developed widely in Europe, USA, Japan, Korea, and so on. However, the current limited data rates and the overload of radio frequency (RF) spectrum are problems which the developers for HAPs are confronting because most of them use RF links to communicate with the ground stations (GSs) or each other. In this paper, we propose an all-optical two-way half-duplex relaying free-space optical (FSO) communication for HAP-based backhaul networks, which connect the base transceiver station (BTS) to the core network (CN) via a single HAP. Our proposed backhaul solution can be deployed quickly and flexibly for disaster relief and for serving users in both urban environments and remote areas. The key subsystem of HAP is an optical regenerate-and-forward (ORF) equipped with an optical hard-limiter (OHL) and an optical XOR gate to perform all-optical processing and help mitigate the background noise. In addition, two-way half-duplex relaying can be provided thanks to the use of network coding scheme. The closed-form expression for the bit error rate (BER) of our proposed system under the effect of path loss, atmospheric turbulence, and noise induced by the background light is formulated. The numerical results are demonstrated to prove the feasibility of our proposed system with the verification by using Monte-Carlo (M-C) simulations.

Enhancing fire science exchange: The Northern Rockies Fire Science Network [poster

Treesearch

Vita Wright

2011-01-01

The Joint Fire Science Program is developing a national network of knowledge exchange consortia comprised of interested management and science stakeholders working together to tailor and actively demonstrate existing fire science information to benefit management.

Effects of amplitude distortions and IF equalization on satellite communication system bit-error rate performance

NASA Technical Reports Server (NTRS)

Kerczewski, Robert J.; Fujikawa, Gene; Svoboda, James S.; Lizanich, Paul J.

1990-01-01

Satellite communications links are subject to distortions which result in an amplitude versus frequency response which deviates from the ideal flat response. Such distortions result from propagation effects such as multipath fading and scintillation and from transponder and ground terminal hardware imperfections. Bit-error rate (BER) degradation resulting from several types of amplitude response distortions were measured. Additional tests measured the amount of BER improvement obtained by flattening the amplitude response of a distorted laboratory simulated satellite channel. The results of these experiments are presented.

Erfassung tribologischer Zusammenhänge und Erkenntnisse in einer Datenbank

NASA Astrophysics Data System (ADS)

Gold, Peter Werner; Jacobs, Georg; Loos, J.; Rombach, Volker; Kurutas, Savas; Fröde, Astrid

Die Datenbank enthält die tribologischen und stofflichen Daten, die innerhalb des Sonderforschungsbereichs 442 ermittelt wurden. Sie wird genutzt, um Informationen über die Eigenschaften von Schmierstoffen und Werkstoffverbunden, sowie über Prüfstände und Versuchsergebnisse zu erhalten. Diese Wissensbasis kann dazu beitragen, ökologisch verträgliche Tribosysteme mit Hilfe der Auswerte-Systeme (Module) auszulegen. Dabei handelt es sich um Berechnungswerkzeuge und Expertenwissen z. B. in Bezug auf die Auswahl von Schichtsystemen. Eine nähere Beschreibung der Auswerte-Systeme kann den folgenden Abschnitten entnommen werden.

Symmetriebrechung und Emergenz in der Kosmologie.

NASA Astrophysics Data System (ADS)

Mainzer, K.

Seit der Antike wird der Aufbau des Universums mit einfachen und regulären (symmetrischen) Grundstrukturen verbunden. Diese Annahme liegt selbst noch den Standardmodellen der relativistischen Kosmologie zugrunde. Demgegenüber läßt sich die Emergenz neuer Strukturen von den Elementarteilchen über Moleküle bis zu den komplexen Systemen des Lebens als Symmetriebrechung verstehen. Symmetriebrechung und strukturelle Komplexität bestimmen die kosmische Evolution. Damit zeichnet sich ein fachübergreifendes Forschungsprogramm von Physik, Chemie und Biologie ab, in dem die Evolution des Universums untersucht werden kann.

Wideband 1.064 micrometer detector evaluation. [for application to space laser communication systems

NASA Technical Reports Server (NTRS)

Green, S. I.

1977-01-01

Several types of communications detectors for use in a 400 Mbps 1.064 micrometer laser communication system were evaluated and characterized. The communication system Bit Error Rate (BER) performance was measured, and test results for the best detector of each type are summarized. The complete BER curves are presented. The 400 Mbps 1.064 micrometer communication system receiver test bed is described. The best performance levels which can be achieved by focusing the signal to diffraction limited spots on the photosensitive area are cited.

An implementation and analysis of the Abstract Syntax Notation One and the basic encoding rules

NASA Technical Reports Server (NTRS)

Harvey, James D.; Weaver, Alfred C.

1990-01-01

The details of abstract syntax notation one standard (ASN.1) and the basic encoding rules standard (BER) that collectively solve the problem of data transfer across incompatible host environments are presented, and a compiler that was built to automate their use is described. Experiences with this compiler are also discussed which provide a quantitative analysis of the performance costs associated with the application of these standards. An evaluation is offered as to how well suited ASN.1 and BER are in solving the common data representation problem.

Human Behavior Modeling in Network Science

DTIC Science & Technology

2010-03-01

in Network Science bringing three distinct research areas together, communication networks, information networks, and social /cognitive networks. The...researchers. A critical part of the social /cognitive network effort is the modeling of human behavior. The modeling efforts range from organizational...behavior to social cognitive trust to explore and refine the theoretical and applied network relationships between and among the human

Two years of LCOGT operations: the challenges of a global observatory

NASA Astrophysics Data System (ADS)

Volgenau, Nikolaus; Boroson, Todd

2016-07-01

With 18 telescopes distributed over 6 sites, and more telescopes being added in 2016, Las Cumbres Observatory Global Telescope Network is a unique resource for timedomain astronomy. The Network's continuous coverage of the night sky, and the optimization of the observing schedule over all sites simultaneously, have enabled LCOGTusers to produce significant science results. However, practical challenges to maximizing the Network's science output remain. The Network began providing observations for members of its Science Collaboration and other partners in May 2014. In the two years since then, LCOGT has made a number of improvements to increase the Network's science yield. We also now have two years' experience monitoring observatory performance; effective monitoring of an observatory that spans the globe is a complex enterprise. Here, we describe some of LCOGT's efforts to monitor the Network, assess the quality of science data, and improve communication with our users.

Joint Transmitter and Receiver Power Allocation under Minimax MSE Criterion with Perfect and Imperfect CSI for MC-CDMA Transmissions

NASA Astrophysics Data System (ADS)

Kotchasarn, Chirawat; Saengudomlert, Poompat

We investigate the problem of joint transmitter and receiver power allocation with the minimax mean square error (MSE) criterion for uplink transmissions in a multi-carrier code division multiple access (MC-CDMA) system. The objective of power allocation is to minimize the maximum MSE among all users each of which has limited transmit power. This problem is a nonlinear optimization problem. Using the Lagrange multiplier method, we derive the Karush-Kuhn-Tucker (KKT) conditions which are necessary for a power allocation to be optimal. Numerical results indicate that, compared to the minimum total MSE criterion, the minimax MSE criterion yields a higher total MSE but provides a fairer treatment across the users. The advantages of the minimax MSE criterion are more evident when we consider the bit error rate (BER) estimates. Numerical results show that the minimax MSE criterion yields a lower maximum BER and a lower average BER. We also observe that, with the minimax MSE criterion, some users do not transmit at full power. For comparison, with the minimum total MSE criterion, all users transmit at full power. In addition, we investigate robust joint transmitter and receiver power allocation where the channel state information (CSI) is not perfect. The CSI error is assumed to be unknown but bounded by a deterministic value. This problem is formulated as a semidefinite programming (SDP) problem with bilinear matrix inequality (BMI) constraints. Numerical results show that, with imperfect CSI, the minimax MSE criterion also outperforms the minimum total MSE criterion in terms of the maximum and average BERs.

Machine Learning Techniques for the Detection of Shockable Rhythms in Automated External Defibrillators

PubMed Central

Irusta, Unai; Morgado, Eduardo; Aramendi, Elisabete; Ayala, Unai; Wik, Lars; Kramer-Johansen, Jo; Eftestøl, Trygve; Alonso-Atienza, Felipe

2016-01-01

Early recognition of ventricular fibrillation (VF) and electrical therapy are key for the survival of out-of-hospital cardiac arrest (OHCA) patients treated with automated external defibrillators (AED). AED algorithms for VF-detection are customarily assessed using Holter recordings from public electrocardiogram (ECG) databases, which may be different from the ECG seen during OHCA events. This study evaluates VF-detection using data from both OHCA patients and public Holter recordings. ECG-segments of 4-s and 8-s duration were analyzed. For each segment 30 features were computed and fed to state of the art machine learning (ML) algorithms. ML-algorithms with built-in feature selection capabilities were used to determine the optimal feature subsets for both databases. Patient-wise bootstrap techniques were used to evaluate algorithm performance in terms of sensitivity (Se), specificity (Sp) and balanced error rate (BER). Performance was significantly better for public data with a mean Se of 96.6%, Sp of 98.8% and BER 2.2% compared to a mean Se of 94.7%, Sp of 96.5% and BER 4.4% for OHCA data. OHCA data required two times more features than the data from public databases for an accurate detection (6 vs 3). No significant differences in performance were found for different segment lengths, the BER differences were below 0.5-points in all cases. Our results show that VF-detection is more challenging for OHCA data than for data from public databases, and that accurate VF-detection is possible with segments as short as 4-s. PMID:27441719

Machine Learning Techniques for the Detection of Shockable Rhythms in Automated External Defibrillators.

PubMed

Figuera, Carlos; Irusta, Unai; Morgado, Eduardo; Aramendi, Elisabete; Ayala, Unai; Wik, Lars; Kramer-Johansen, Jo; Eftestøl, Trygve; Alonso-Atienza, Felipe

2016-01-01

Early recognition of ventricular fibrillation (VF) and electrical therapy are key for the survival of out-of-hospital cardiac arrest (OHCA) patients treated with automated external defibrillators (AED). AED algorithms for VF-detection are customarily assessed using Holter recordings from public electrocardiogram (ECG) databases, which may be different from the ECG seen during OHCA events. This study evaluates VF-detection using data from both OHCA patients and public Holter recordings. ECG-segments of 4-s and 8-s duration were analyzed. For each segment 30 features were computed and fed to state of the art machine learning (ML) algorithms. ML-algorithms with built-in feature selection capabilities were used to determine the optimal feature subsets for both databases. Patient-wise bootstrap techniques were used to evaluate algorithm performance in terms of sensitivity (Se), specificity (Sp) and balanced error rate (BER). Performance was significantly better for public data with a mean Se of 96.6%, Sp of 98.8% and BER 2.2% compared to a mean Se of 94.7%, Sp of 96.5% and BER 4.4% for OHCA data. OHCA data required two times more features than the data from public databases for an accurate detection (6 vs 3). No significant differences in performance were found for different segment lengths, the BER differences were below 0.5-points in all cases. Our results show that VF-detection is more challenging for OHCA data than for data from public databases, and that accurate VF-detection is possible with segments as short as 4-s.

Dual-polarization multi-band optical OFDM transmission and transceiver limitations for up to 500 Gb/s uncompensated long-haul links.

PubMed

Giacoumidis, E; Jarajreh, M A; Sygletos, S; Le, S T; Farjady, F; Tsokanos, A; Hamié, A; Pincemin, E; Jaouën, Y; Ellis, A D; Doran, N J

2014-05-05

A number of critical issues for dual-polarization single- and multi-band optical orthogonal-frequency division multiplexing (DP-SB/MB-OFDM) signals are analyzed in dispersion compensation fiber (DCF)-free long-haul links. For the first time, different DP crosstalk removal techniques are compared, the maximum transmission-reach is investigated, and the impact of subcarrier number and high-level modulation formats are explored thoroughly. It is shown, for a bit-error-rate (BER) of 10(-3), 2000 km of quaternary phase-shift keying (QPSK) DP-MB-OFDM transmission is feasible. At high launched optical powers (LOP), maximum-likelihood decoding can extend the LOP of 40 Gb/s QPSK DP-SB-OFDM at 2000 km by 1.5 dB compared to zero-forcing. For a 100 Gb/s DP-MB-OFDM system, a high number of subcarriers contribute to improved BER but at the cost of digital signal processing computational complexity, whilst by adapting the cyclic prefix length the BER can be improved for a low number of subcarriers. In addition, when 16-quadrature amplitude modulation (16QAM) is employed the digital-to-analogue/analogue-to-digital converter (DAC/ADC) bandwidth is relaxed with a degraded BER; while the 'circular' 8QAM is slightly superior to its 'rectangular' form. Finally, the transmission of wavelength-division multiplexing DP-MB-OFDM and single-carrier DP-QPSK is experimentally compared for up to 500 Gb/s showing great potential and similar performance at 1000 km DCF-free G.652 line.

Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress

PubMed Central

Sobol, Robert W.; Watson, David E.; Nakamura, Jun; Yakes, F. Michael; Hou, Esther; Horton, Julie K.; Ladapo, Joseph; Van Houten, Bennett; Swenberg, James A.; Tindall, Kenneth R.; Samson, Leona D.; Wilson, Samuel H.

2002-01-01

The long-term effect of exposure to DNA alkylating agents is entwined with the cell's genetic capacity for DNA repair and appropriate DNA damage responses. A unique combination of environmental exposure and deficiency in these responses can lead to genomic instability; this “gene–environment interaction” paradigm is a theme for research on chronic disease etiology. In the present study, we used mouse embryonic fibroblasts with a gene deletion in the base excision repair (BER) enzymes DNA β-polymerase (β-pol) and alkyladenine DNA glycosylase (AAG), along with exposure to methyl methanesulfonate (MMS) to study mutagenesis as a function of a particular gene–environment interaction. The β-pol null cells, defective in BER, exhibit a modest increase in spontaneous mutagenesis compared with wild-type cells. MMS exposure increases mutant frequency in β-pol null cells, but not in isogenic wild-type cells; UV light exposure or N-methyl-N′-nitro-N-nitrosoguanidine exposure increases mutant frequency similarly in both cell lines. The MMS-induced increase in mutant frequency in β-pol null cells appears to be caused by DNA lesions that are AAG substrates, because overexpression of AAG in β-pol null cells eliminates the effect. In contrast, β-pol/AAG double null cells are slightly more mutable than the β-pol null cells after MMS exposure. These results illustrate that BER plays a role in protecting mouse embryonic fibroblast cells against methylation-induced mutations and characterize the effect of a particular combination of BER gene defect and environmental exposure. PMID:11983862

Differential role of base excision repair proteins in mediating cisplatin cytotoxicity.

PubMed

Sawant, Akshada; Floyd, Ashley M; Dangeti, Mohan; Lei, Wen; Sobol, Robert W; Patrick, Steve M

2017-03-01

Interstrand crosslinks (ICLs) are covalent lesions formed by cisplatin. The mechanism for the processing and removal of ICLs by DNA repair proteins involves nucleotide excision repair (NER), homologous recombination (HR) and fanconi anemia (FA) pathways. In this report, we monitored the processing of a flanking uracil adjacent to a cisplatin ICL by the proteins involved in the base excision repair (BER) pathway. Using a combination of extracts, purified proteins, inhibitors, functional assays and cell culture studies, we determined the specific BER proteins required for processing a DNA substrate with a uracil adjacent to a cisplatin ICL. Uracil DNA glycosylase (UNG) is the primary glycosylase responsible for the removal of uracils adjacent to cisplatin ICLs, whereas other uracil glycosylases can process uracils in the context of undamaged DNA. Repair of the uracil adjacent to cisplatin ICLs proceeds through the classical BER pathway, highlighting the importance of specific proteins in this redundant pathway. Removal of uracil is followed by the generation of an abasic site and subsequent cleavage by AP endonuclease 1 (APE1). Inhibition of either the repair or redox domain of APE1 gives rise to cisplatin resistance. Inhibition of the lyase domain of Polymerase β (Polβ) does not influence cisplatin cytotoxicity. In addition, lack of XRCC1 leads to increased DNA damage and results in increased cisplatin cytotoxicity. Our results indicate that BER activation at cisplatin ICLs influences crosslink repair and modulates cisplatin cytotoxicity via specific UNG, APE1 and Polβ polymerase functions. Copyright © 2017 Elsevier B.V. All rights reserved.

Loosening the shackles of scientific disciplines with network science. Reply to comments on "Network science of biological systems at different scales: A review"

NASA Astrophysics Data System (ADS)

Gosak, Marko; Markovič, Rene; Dolenšek, Jurij; Rupnik, Marjan Slak; Marhl, Marko; Stožer, Andraž; Perc, Matjaž

2018-03-01

We would like to thank all the experts for their insightful and very interesting comments that have been submitted in response to our review "Network science of biological systems at different scales" [1]. We are delighted with the number of comments that have been written, and even more so with the positive opinions that these comments communicate to the wider audience [2-9]. Although methods of network science have long proven their value in relevantly addressing various challenges in the biological sciences, such interdisciplinary research often still struggles for funding and recognition at many academic levels.

Building a Networked Improvement Community to Promote Equitable, Coherent Systems of Science Education: How a State-Level Team Can Support District-Level Change Efforts

ERIC Educational Resources Information Center

Penuel, William R.; Shaw, Sam; Bell, Philip; Hopkins, Megan; Neill, Tiffany; Farrell, Caitlin C.

2018-01-01

This paper describes a Networked Improvement Community comprised of a network of 13 states focused on improving coherence and equity in state systems of science education. Grounded in principles of improvement science adapted from healthcare, we are developing and testing resources for formative assessment in science, with the aim of developing…

DNA polymerase β variant Ile260Met generates global gene expression changes related to cellular transformation

PubMed Central

Sweasy, Joann B.

2012-01-01

Maintenance of genomic stability is essential for cellular survival. The base excision repair (BER) pathway is critical for resolution of abasic sites and damaged bases, estimated to occur 20,000 times in cells daily. DNA polymerase β (Pol β) participates in BER by filling DNA gaps that result from excision of damaged bases. Approximately 30% of human tumours express Pol β variants, many of which have altered fidelity and activity in vitro and when expressed, induce cellular transformation. The prostate tumour variant Ile260Met transforms cells and is a sequence-context-dependent mutator. To test the hypothesis that mutations induced in vivo by Ile260Met lead to cellular transformation, we characterized the genome-wide expression profile of a clone expressing Ile260Met as compared with its non-induced counterpart. Using a 1.5-fold minimum cut-off with a false discovery rate (FDR) of <0.05, 912 genes exhibit altered expression. Microarray results were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and revealed unique expression profiles in other clones. Gene Ontology (GO) clusters were analyzed using Ingenuity Pathways Analysis to identify altered gene networks and associated nodes. We determined three nodes of interest that exhibited dysfunctional regulation of downstream gene products without themselves having altered expression. One node, peroxisome proliferator-activated protein γ (PPARG), was sequenced and found to contain a coding region mutation in PPARG2 only in transformed cells. Further analysis suggests that this mutation leads to dominant negative activity of PPARG2. PPARG is a transcription factor implicated to have tumour suppressor function. This suggests that the PPARG2 mutant may have played a role in driving cellular transformation. We conclude that PPARG induces cellular transformation by a mutational mechanism. PMID:22914675

Solar-Terrestrial and Astronomical Research Network (STAR-Network) - A Meaningful Practice of New Cyberinfrastructure on Space Science

NASA Astrophysics Data System (ADS)

Hu, X.; Zou, Z.

2017-12-01

For the next decades, comprehensive big data application environment is the dominant direction of cyberinfrastructure development on space science. To make the concept of such BIG cyberinfrastructure (e.g. Digital Space) a reality, these aspects of capability should be focused on and integrated, which includes science data system, digital space engine, big data application (tools and models) and the IT infrastructure. In the past few years, CAS Chinese Space Science Data Center (CSSDC) has made a helpful attempt in this direction. A cloud-enabled virtual research platform on space science, called Solar-Terrestrial and Astronomical Research Network (STAR-Network), has been developed to serve the full lifecycle of space science missions and research activities. It integrated a wide range of disciplinary and interdisciplinary resources, to provide science-problem-oriented data retrieval and query service, collaborative mission demonstration service, mission operation supporting service, space weather computing and Analysis service and other self-help service. This platform is supported by persistent infrastructure, including cloud storage, cloud computing, supercomputing and so on. Different variety of resource are interconnected: the science data can be displayed on the browser by visualization tools, the data analysis tools and physical models can be drived by the applicable science data, the computing results can be saved on the cloud, for example. So far, STAR-Network has served a series of space science mission in China, involving Strategic Pioneer Program on Space Science (this program has invested some space science satellite as DAMPE, HXMT, QUESS, and more satellite will be launched around 2020) and Meridian Space Weather Monitor Project. Scientists have obtained some new findings by using the science data from these missions with STAR-Network's contribution. We are confident that STAR-Network is an exciting practice of new cyberinfrastructure architecture on space science.

Network science of biological systems at different scales: A review

NASA Astrophysics Data System (ADS)

Gosak, Marko; Markovič, Rene; Dolenšek, Jurij; Slak Rupnik, Marjan; Marhl, Marko; Stožer, Andraž; Perc, Matjaž

2018-03-01

Network science is today established as a backbone for description of structure and function of various physical, chemical, biological, technological, and social systems. Here we review recent advances in the study of complex biological systems that were inspired and enabled by methods of network science. First, we present

Pseudodesulfovibrio hydrargyri sp. nov., a mercury-methylating bacterium isolated from a brackish sediment.

PubMed

Ranchou-Peyruse, Magali; Goñi-Urriza, Marisol; Guignard, Marion; Goas, Marjorie; Ranchou-Peyruse, Anthony; Guyoneaud, Rémy

2018-05-01

The strain BerOc1 T was isolated from brackish sediments contaminated with hydrocarbons and heavy metals. This strain has been used as a model strain of sulfate-reducer to study the biomethylation of mercury. The cells are vibrio-shaped, motile and not sporulated. Phylogeny and physiological traits placed this strain within the genus Pseudodesulfovibrio. Optimal growth was obtained at 30 °C, 1.5 % NaCl and pH 6.0-7.4. The estimated G+C content of the genomic DNA was 62.6 mol%. BerOc1 T used lactate, pyruvate, fumarate, ethanol and hydrogen. Terminal electron acceptors used were sulfate, sulfite, thiosulfate and DMSO. Only pyruvate could be used without a terminal electron acceptor. The major fatty acids were C18 : 0, anteiso-C15 : 0, C16 : 0 and C18 : 1ω7. The name Pseudodesulfovibrio hydrargyri sp. nov. is proposed for the type strain BerOc1 T (DSM 10384 T =JCM 31820 T ).

Analyzing the propagation behavior of scintillation index and bit error rate of a partially coherent flat-topped laser beam in oceanic turbulence.

PubMed

Yousefi, Masoud; Golmohammady, Shole; Mashal, Ahmad; Kashani, Fatemeh Dabbagh

2015-11-01

In this paper, on the basis of the extended Huygens-Fresnel principle, a semianalytical expression for describing on-axis scintillation index of a partially coherent flat-topped (PCFT) laser beam of weak to moderate oceanic turbulence is derived; consequently, by using the log-normal intensity probability density function, the bit error rate (BER) is evaluated. The effects of source factors (such as wavelength, order of flatness, and beam width) and turbulent ocean parameters (such as Kolmogorov microscale, relative strengths of temperature and salinity fluctuations, rate of dissipation of the mean squared temperature, and rate of dissipation of the turbulent kinetic energy per unit mass of fluid) on propagation behavior of scintillation index, and, hence, on BER, are studied in detail. Results indicate that, in comparison with a Gaussian beam, a PCFT laser beam with a higher order of flatness is found to have lower scintillations. In addition, the scintillation index and BER are most affected when salinity fluctuations in the ocean dominate temperature fluctuations.

Hide and seek: How do DNA glycosylases locate oxidatively damaged DNA bases amidst a sea of undamaged bases?

PubMed

Lee, Andrea J; Wallace, Susan S

2017-06-01

The first step of the base excision repair (BER) pathway responsible for removing oxidative DNA damage utilizes DNA glycosylases to find and remove the damaged DNA base. How glycosylases find the damaged base amidst a sea of undamaged bases has long been a question in the BER field. Single molecule total internal reflection fluorescence microscopy (SM TIRFM) experiments have allowed for an exciting look into this search mechanism and have found that DNA glycosylases scan along the DNA backbone in a bidirectional and random fashion. By comparing the search behavior of bacterial glycosylases from different structural families and with varying substrate specificities, it was found that glycosylases search for damage by periodically inserting a wedge residue into the DNA stack as they redundantly search tracks of DNA that are 450-600bp in length. These studies open up a wealth of possibilities for further study in real time of the interactions of DNA glycosylases and other BER enzymes with various DNA substrates. Copyright © 2016 Elsevier Inc. All rights reserved.

Determining blood and plasma volumes using bioelectrical response spectroscopy

NASA Technical Reports Server (NTRS)

Siconolfi, S. F.; Nusynowitz, M. L.; Suire, S. S.; Moore, A. D. Jr; Leig, J.

1996-01-01

We hypothesized that an electric field (inductance) produced by charged blood components passing through the many branches of arteries and veins could assess total blood volume (TBV) or plasma volume (PV). Individual (N = 29) electrical circuits (inductors, two resistors, and a capacitor) were determined from bioelectrical response spectroscopy (BERS) using a Hewlett Packard 4284A Precision LCR Meter. Inductance, capacitance, and resistance from the circuits of 19 subjects modeled TBV (sum of PV and computed red cell volume) and PV (based on 125I-albumin). Each model (N = 10, cross validation group) had good validity based on 1) mean differences (-2.3 to 1.5%) between the methods that were not significant and less than the propagated errors (+/- 5.2% for TBV and PV), 2) high correlations (r > 0.92) with low SEE (< 7.7%) between dilution and BERS assessments, and 3) Bland-Altman pairwise comparisons that indicated "clinical equivalency" between the methods. Given the limitation of this study (10 validity subjects), we concluded that BERS models accurately assessed TBV and PV. Further evaluations of the models' validities are needed before they are used in clinical or research settings.

Schönheit und andere Provokationen - Eine neue evolutionsbiologische Theorie der Kunst

NASA Astrophysics Data System (ADS)

Junker, Thomas

Die Evolution hat viele spektakuläre Phänomene hervorgebracht - von der Eleganz des Vogelflugs über die gigantischen Körper der Dinosaurier und die farbenprächtige Vielfalt der Korallenriffe bis hin zu ihrem jüngsten Geniestreich - der menschlichen Kunst. Die schönen Künste - Malerei, Bildhauerei und Architektur, Theater, Tanz, Oper und Filmkunst, Musik und Literatur - Produkte der Evolution? Diese Vorstellung mutet vielen Menschen fremd an, aber wie könnte es anders sein? Denn wenn Charles Darwin recht hat, dann sind nicht nur die körperlichen Merkmale der Menschen als Antworten auf die Erfordernisse des Lebens entstanden, sondern auch ihre geistigen Fähigkeiten und Verhaltensweisen. Im Jahr 1859 hatte er auf den letzten Seiten seines berühmten Buches über die Entstehung der Arten eine kühne Prophezeiung gemacht: Durch die Evolutionstheorie werde es "zu einer bemerkenswerten Revolution in der Naturwissenschaft kommen […]. Die Psychologie wird auf die neue Grundlage gestellt, dass jede geistige Kraft und Fähigkeit notwendigerweise durch graduelle Übergänge erworben wird“ (Darwin 1859, S. 484, 488; Junker 2008).

Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia).

PubMed

Landmann, Christian; Fink, Barbara; Festner, Maria; Dregus, Márta; Engel, Karl-Heinz; Schwab, Wilfried

2007-09-15

The essential oil of lavender (Lavandula angustifolia) is mainly composed of mono- and sesquiterpenes. Using a homology-based PCR strategy, two monoterpene synthases (LaLIMS and LaLINS) and one sesquiterpene synthase (LaBERS) were cloned from lavender leaves and flowers. LaLIMS catalyzed the formation of (R)-(+)-limonene, terpinolene, (1R,5S)-(+)-camphene, (1R,5R)-(+)-alpha-pinene, beta-myrcene and traces of alpha-phellandrene. The proportions of these products changed significantly when Mn(2+) was supplied as the cofactor instead of Mg(2+). The second enzyme LaLINS produced exclusively (R)-(-)-linalool, the main component of lavender essential oil. LaBERS transformed farnesyl diphosphate and represents the first reported trans-alpha-bergamotene synthase. It accepted geranyl diphosphate with higher affinity than farnesyl diphosphate and also produced monoterpenes, albeit at low rates. LaBERS is probably derived from a parental monoterpene synthase by the loss of the plastidial signal peptide and by broadening its substrate acceptance spectrum. The identification and description of the first terpene synthases from L. angustifolia forms the basis for the biotechnological modification of essential oil composition in lavender.

Base excision repair, the redox environment and therapeutic implications.

PubMed

Storr, S J; Woolston, C M; Martin, S G

2012-01-01

Control of redox homeostasis is crucial for a number of cellular processes with deregulation leading to a number of serious consequences including oxidative damage such induction of DNA base lesions. The DNA lesions caused by oxidative damage are principally repaired by the base excision repair (BER) pathway. Pharmacological inhibition of BER is becoming an increasingly active area of research with the emergence of PARP inhibitors in cancer therapy. The redox status of the cell is modulated by a number of systems, including a large number of anti-oxidant enzymes who function in the control of superoxide and hydrogen peroxide, and ultimately in the release of the damaging hydroxyl radical. Here we provide an overview of reactive oxygen species (ROS) production and its modulation by antioxidant enzymes. The review also discusses the effect of ROS on the BER pathway, particularly in relation to cancer. Finally, as the modulation of the redox environment is of interest in cancer therapy, with certain agents having the potential to reverse chemo- and radiotherapy resistance or treat therapy related toxicity, we discuss redox modulating agents currently under development.

Performance analysis of EM-based blind detection for ON-OFF keying modulation over atmospheric optical channels

NASA Astrophysics Data System (ADS)

Dabiri, Mohammad Taghi; Sadough, Seyed Mohammad Sajad

2018-04-01

In the free-space optical (FSO) links, atmospheric turbulence lead to scintillation in the received signal. Due to its ease of implementation, intensity modulation with direct detection (IM/DD) based on ON-OFF keying (OOK) is a popular signaling scheme in these systems. Over turbulence channel, to detect OOK symbols in a blind way, i.e., without sending pilot symbols, an expectation-maximization (EM)-based detection method was recently proposed in the literature related to free-space optical (FSO) communication. However, the performance of EM-based detection methods severely depends on the length of the observation interval (Ls). To choose the optimum values of Ls at target bit error rates (BER)s of FSO communications which are commonly lower than 10-9, Monte-Carlo simulations would be very cumbersome and require a very long processing time. To facilitate performance evaluation, in this letter we derive the analytic expressions for BER and outage probability. Numerical results validate the accuracy of our derived analytic expressions. Our results may serve to evaluate the optimum value for Ls without resorting to time-consuming Monte-Carlo simulations.

A joint technique for sidelobe suppression and peak-to-average power ratio reduction in non-contiguous OFDM-based cognitive radio networks

NASA Astrophysics Data System (ADS)

Sivadas, Namitha Arackal; Mohammed, Sameer Saheerudeen

2017-02-01

In non-contiguous orthogonal frequency division multiplexing (NC-OFDM)-based interweave cognitive radio networks, the sidelobe power of secondary users (SUs) must be strictly controlled to avoid the interference between the SUs and the primary users (PUs) of the adjacent bands. Similarly, the inherent issue of high peak-to-average power ratio (PAPR) of the OFDM signal is another drawback of the cognitive radio communication system based on the NC-OFDM technology. A few methods are available in the literature to solve either of these problems individually, while in this paper, we propose a new method for the joint minimisation of sidelobe power and PAPR in NC-OFDM-based cognitive radio networks using Zadoff-Chu (ZC) sequence. In this method, the sidelobe power suppression of SUs is benefited by PUs and the PAPR is reduced for SUs. We modelled a new optimisation problem for minimising the sidelobe power with a constraint on the maximum tolerable PAPR and sidelobe power. The proper selection of ZC sequence, which is crucial for minimising both the issues simultaneously, is achieved by solving the proposed optimisation problem. The proposed technique is shown to provide 7 dB and 20 dB reduction in PAPR and sidelobe power, respectively, without causing any signal distortion along with the improvement in bit error rate (BER) performance.

The transmission of symmetric 40 Gb/s TWDM-based NG-PON2 utilizing delay interferometer (DI) for RSOA bandwidth enhancement

NASA Astrophysics Data System (ADS)

Bindhaiq, Salem; Zulkifli, Nadiatulhuda; Supa'at, AbuSahmah M.

2016-07-01

Time and wavelength-division multiplexed passive optical network (TWDM-PON) has been finally selected as the pragmatic solution for the next-generation passive optical network stage 2 (NG-PON2). In this paper, we propose a symmetric 40 Gb/s TWDM-PON system with low cost reflective semiconductor optical amplifier (RSOA) for both downstream and upstream directions. A single bi-pass delay interferometer (DI), deployed in the optical line terminal (OLT), is used to enhance the poor performance of the RSOA with respect to the low bandwidth induced by laser chirp. With the help of the 40 GHz free spectrum range (FSR) DI, we show a successful transmission of the proposed work through simulation study where an aggregate capacity of 40 Gb/s is transported over 40 km transmission distance with 32 splits. The TWDM-PON system at BER of 10-6 has shown a minimum receiver sensitivity of -22.78 dBm and -22.71 dBm for both downstream and upstream, respectively with maximum power penalty of 2 dB for downstream channel and 2.39 dB for upstream channel.

Joint Transmit Power Allocation and Splitting for SWIPT Aided OFDM-IDMA in Wireless Sensor Networks

PubMed Central

Li, Shanshan; Zhou, Xiaotian; Wang, Cheng-Xiang; Yuan, Dongfeng; Zhang, Wensheng

2017-01-01

In this paper, we propose to combine Orthogonal Frequency Division Multiplexing-Interleave Division Multiple Access (OFDM-IDMA) with Simultaneous Wireless Information and Power Transfer (SWIPT), resulting in SWIPT aided OFDM-IDMA scheme for power-limited sensor networks. In the proposed system, the Receive Node (RN) applies Power Splitting (PS) to coordinate the Energy Harvesting (EH) and Information Decoding (ID) process, where the harvested energy is utilized to guarantee the iterative Multi-User Detection (MUD) of IDMA to work under sufficient number of iterations. Our objective is to minimize the total transmit power of Source Node (SN), while satisfying the requirements of both minimum harvested energy and Bit Error Rate (BER) performance from individual receive nodes. We formulate such a problem as a joint power allocation and splitting one, where the iteration number of MUD is also taken into consideration as the key parameter to affect both EH and ID constraints. To solve it, a sub-optimal algorithm is proposed to determine the power profile, PS ratio and iteration number of MUD in an iterative manner. Simulation results verify that the proposed algorithm can provide significant performance improvement. PMID:28677636

Joint Transmit Power Allocation and Splitting for SWIPT Aided OFDM-IDMA in Wireless Sensor Networks.

PubMed

Li, Shanshan; Zhou, Xiaotian; Wang, Cheng-Xiang; Yuan, Dongfeng; Zhang, Wensheng

2017-07-04

In this paper, we propose to combine Orthogonal Frequency Division Multiplexing-Interleave Division Multiple Access (OFDM-IDMA) with Simultaneous Wireless Information and Power Transfer (SWIPT), resulting in SWIPT aided OFDM-IDMA scheme for power-limited sensor networks. In the proposed system, the Receive Node (RN) applies Power Splitting (PS) to coordinate the Energy Harvesting (EH) and Information Decoding (ID) process, where the harvested energy is utilized to guarantee the iterative Multi-User Detection (MUD) of IDMA to work under sufficient number of iterations. Our objective is to minimize the total transmit power of Source Node (SN), while satisfying the requirements of both minimum harvested energy and Bit Error Rate (BER) performance from individual receive nodes. We formulate such a problem as a joint power allocation and splitting one, where the iteration number of MUD is also taken into consideration as the key parameter to affect both EH and ID constraints. To solve it, a sub-optimal algorithm is proposed to determine the power profile, PS ratio and iteration number of MUD in an iterative manner. Simulation results verify that the proposed algorithm can provide significant performance improvement.

Topological Interference Management for K-User Downlink Massive MIMO Relay Network Channel.

PubMed

Selvaprabhu, Poongundran; Chinnadurai, Sunil; Li, Jun; Lee, Moon Ho

2017-08-17

In this paper, we study the emergence of topological interference alignment and the characterizing features of a multi-user broadcast interference relay channel. We propose an alternative transmission strategy named the relay space-time interference alignment (R-STIA) technique, in which a K -user multiple-input-multiple-output (MIMO) interference channel has massive antennas at the transmitter and relay. Severe interference from unknown transmitters affects the downlink relay network channel and degrades the system performance. An additional (unintended) receiver is introduced in the proposed R-STIA technique to overcome the above problem, since it has the ability to decode the desired signals for the intended receiver by considering cooperation between the receivers. The additional receiver also helps in recovering and reconstructing the interference signals with limited channel state information at the relay (CSIR). The Alamouti space-time transmission technique and minimum mean square error (MMSE) linear precoder are also used in the proposed scheme to detect the presence of interference signals. Numerical results show that the proposed R-STIA technique achieves a better performance in terms of the bit error rate (BER) and sum-rate compared to the existing broadcast channel schemes.

Topological Interference Management for K-User Downlink Massive MIMO Relay Network Channel

PubMed Central

Li, Jun; Lee, Moon Ho

2017-01-01

In this paper, we study the emergence of topological interference alignment and the characterizing features of a multi-user broadcast interference relay channel. We propose an alternative transmission strategy named the relay space-time interference alignment (R-STIA) technique, in which a K-user multiple-input-multiple-output (MIMO) interference channel has massive antennas at the transmitter and relay. Severe interference from unknown transmitters affects the downlink relay network channel and degrades the system performance. An additional (unintended) receiver is introduced in the proposed R-STIA technique to overcome the above problem, since it has the ability to decode the desired signals for the intended receiver by considering cooperation between the receivers. The additional receiver also helps in recovering and reconstructing the interference signals with limited channel state information at the relay (CSIR). The Alamouti space-time transmission technique and minimum mean square error (MMSE) linear precoder are also used in the proposed scheme to detect the presence of interference signals. Numerical results show that the proposed R-STIA technique achieves a better performance in terms of the bit error rate (BER) and sum-rate compared to the existing broadcast channel schemes. PMID:28817071

Overview of NASA communications infrastructure

NASA Technical Reports Server (NTRS)

Arnold, Ray J.; Fuechsel, Charles

1991-01-01

The infrastructure of NASA communications systems for effecting coordination across NASA offices and with the national and international research and technological communities is discussed. The offices and networks of the communication system include the Office of Space Science and Applications (OSSA), which manages all NASA missions, and the Office of Space Operations, which furnishes communication support through the NASCOM, the mission critical communications support network, and the Program Support Communications network. The NASA Science Internet was established by OSSA to centrally manage, develop, and operate an integrated computer network service dedicated to NASA's space science and application research. Planned for the future is the National Research and Education Network, which will provide communications infrastructure to enhance science resources at a national level.

Suppression of Ice Fog from Cooling Ponds

DTIC Science & Technology

1976-11-01

Data evaporation . rnge of spad (gmn2 day") (mm day) C ) meas. (mm day’) Ohtake (1970) 5040 5.0 4 -15 10 0.9- 5.9 Behlke and McDougall (1973) 4464 4.5...plant cooling pond at -23* C . On the left side of the pond ice fog has been nearly eliminated by the formation of an ice cover. (Photograph by Terry...unlimited. 17. cISTRISUTION STATEMIENT (of S. absauat eod Sm BerS8. If 4fforIon vRepot) t. KEY WORDS ( C €t an o rewoosi .e* I eo~ra md identJ by Week

A new LDPC decoding scheme for PDM-8QAM BICM coherent optical communication system

NASA Astrophysics Data System (ADS)

Liu, Yi; Zhang, Wen-bo; Xi, Li-xia; Tang, Xian-feng; Zhang, Xiao-guang

2015-11-01

A new log-likelihood ratio (LLR) message estimation method is proposed for polarization-division multiplexing eight quadrature amplitude modulation (PDM-8QAM) bit-interleaved coded modulation (BICM) optical communication system. The formulation of the posterior probability is theoretically analyzed, and the way to reduce the pre-decoding bit error rate ( BER) of the low density parity check (LDPC) decoder for PDM-8QAM constellations is presented. Simulation results show that it outperforms the traditional scheme, i.e., the new post-decoding BER is decreased down to 50% of that of the traditional post-decoding algorithm.

The Composite Analytic and Simulation Package or RFI (CASPR) on a coded channel

NASA Technical Reports Server (NTRS)

Freedman, Jeff; Berman, Ted

1993-01-01

CASPR is an analysis package which determines the performance of a coded signal in the presence of Radio Frequency Interference (RFI) and Additive White Gaussian Noise (AWGN). It can analyze a system with convolutional coding, Reed-Solomon (RS) coding, or a concatenation of the two. The signals can either be interleaved or non-interleaved. The model measures the system performance in terms of either the E(sub b)/N(sub 0) required to achieve a given Bit Error Rate (BER) or the BER needed for a constant E(sub b)/N(sub 0).

Sternbilder und ihre Mythen

NASA Astrophysics Data System (ADS)

Fasching, Gerhard

Die Sternbilder und die damit verbundenen Mythen helfen, sich am Sternenhimmel zurechtzufinden und vermitteln die Vielfalt der Bilder der Mythologie und der Sternsagen. Sternkarten und alte Kupferstiche aus dem Bestand der Österreichischen Nationalbibliothek zeigen, wie man sich in früheren Jahrhunderten den Sternenhimmel vorgestellt hat. Ausführliche Sachverzeichnisse mit über 3000 Suchbegriffen erleichtern den Zugang zu Stern- und Sternbildnamen und zur Mythologie. Die dritte, erweiterte Auflage gibt für die kommenden Jahrzehnte darüber Auskunft, wo und wann die Planeten aber auch Sternhaufen, Gasnebel und Galaxien am Himmel mühelos aufgefunden werden können.

Grundlagen und Grundbegriffe der Messtechnik

NASA Astrophysics Data System (ADS)

Plaßmann, Wilfried

Es ist eine wesentliche Aufgabe der Messtechnik, technische Vorgänge quantitativ zu erfassen und anhand der gemessenen Größen Funktionsabläufe zu steuern. Als Beispiel sei ein Kraftwerk zur Energieerzeugung genannt, bei dem nur über die Messung von Temperaturen, Leistungen, Drücken und anderen Größen Aussagen über den momentanen Zustand möglich sind und bei Abweichungen vom Sollwert geeignete Eingriffe in das System erfolgen können. Damit eine eindeutige Kommunikation möglich wird, sind die in der Messtechnik verwendeten Begriffe, Messverfahren und Maßeinheiten in entsprechenden Normen oder Vorschriften festgelegt.

Taxonomic revision of the Neotropical genus Caenopangonia Kröber, 1930 (Diptera: Tabanidae).

PubMed

Krolow, Tiago Kütter; Henriques, Augusto Loureiro; González, Christian R

2016-08-24

The Neotropical species of Caenopangonia Kröber are revised herein based on examination of external and internal morphology of type material and specimens from Argentina and Chile. Three currently valid species are recognized: Caenopangonia aspera (Philip, 1958), Caenopangonia brevirostris (Philippi, 1865), and Caenopangonia hirtipalpis (Bigot, 1892). Two new species are described, Caenopangonia cerdai n. sp. Krolow, Henriques & González (Chile, Chacabuco) and Caenopangonia coscaroni n. sp. Krolow, Henriques & González (Chile, Malleco). We provide diagnosis and/or descriptions, distribution records, illustrations and discussion for all species, as well a key for identification.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scott, Bobby R.; Tokarskaya, Zoya B.; Zhuntova, Galina V.

This report summarizes 4 years of research achievements in this Office of Science (BER), U.S. Department of Energy (DOE) project. The research described was conducted by scientists and supporting staff at Lovelace Respiratory Research Institute (LRRI)/Lovelace Biomedical and Environmental Research Institute (LBERI) and the Southern Urals Biophysics Institute (SUBI). All project objectives and goals were achieved. A major focus was on obtaining improved cancer risk estimates for exposure via inhalation to plutonium (Pu) isotopes in the workplace (DOE radiation workers) and environment (public exposures to Pu-contaminated soil). A major finding was that low doses and dose rates of gamma raysmore » can significantly suppress cancer induction by alpha radiation from inhaled Pu isotopes. The suppression relates to stimulation of the body's natural defenses, including immunity against cancer cells and selective apoptosis which removes precancerous and other aberrant cells.« less

Otoman encounters with European science: sixtienth and seventeenth-century translations into turkish

NASA Astrophysics Data System (ADS)

Gunegrun, Feza

The article concerns the traslation of medical and geographical texts from Arabic, Persian and European languages during Middle Age. The following astonomical texts are mentioned: a)Si fasl der marifet-i takvim (The thirty Chapters of Calendar Making) b)Er- Risaletu'l-fethiyye fi'l- hey'e (Astronomical treatise glorifying the Triump) by Ali Qushji, translated bySeydi Ali Reis c)The European astronomical Tables by Nathalis Durret (1590-1650)translated by Tezkireci Kose Ibrahim (1635-1663) d)Secenel-al-aflak fi gayet al idrak (Mirror of the Heavens and the Summit of Perception)translated by Tezkireci Kose Ibrahim Effendi. It is noted, that Ottoman astrologers and timekeepers used the tables by Ulug Beg till the mid- eighteenth century. e) The astronomical tables by Alexis Clairot (1713-165) were translated by Helifezade Cinari Ismail Effendi.

Curriculum Framework (CF) Implementation Conference. Report of the Regional Educational Laboratory Network Program and the National Network of Eisenhower Mathematics and Science Regional Consortia (Hilton Head Island, South Carolina, January 26-27, 1995).

ERIC Educational Resources Information Center

Palmer, Jackie; Powell, Mary Jo

The Laboratory Network Program and the National Network of Eisenhower Mathematics and Science Regional Consortia, operating as the Curriculum Frameworks Task Force, jointly convened a group of educators involved in implementing state-level mathematics or science curriculum frameworks (CF). The Hilton Head (South Carolina) conference had a dual…

Network Interdependency Modeling for Risk Assessment on Built Infrastructure Systems

DTIC Science & Technology

2013-10-01

does begin to address infrastructure decay as a source of risk comes from the Department of Homeland Security (DHS). In 2009, the DHS Science and...network of connected edges and nodes. The National Research Council (2005) reported that the study of networks as a science and applications of...principles from this science are still in its early stages. As modern infrastructures have become more interlinked, knowledge of an infrastructure’s network

High-Speed Burst-Mode Clock and Data Recovery Circuits for Multiaccess Networks

NASA Astrophysics Data System (ADS)

Shastri, Bhavin J.

Optical multiaccess networks, and specifically passive optical networks (PONs) are considered to be the most promising technologies for the deployment of fiber-to-the-premises/home/user (FTTx) to solve the problem of limited bandwidth in local area networks with a low-cost solution and a guaranteed quality of service. In a PON, multiple users share the fiber infrastructure in a point-to-multipoint (P2MP) network. This topology introduce optical path delays which inherently cause the data packets to undergo amplitude variations up to 20 dB and phase variations from --2pi to +2pi rad--burst-mode traffic. Consequently, this creates new challenges for the design and test of optical receivers front-ends and clock and data recovery circuits (CDRs), in particular, burst-mode CDRs (BM-CDRs). The research presented in this thesis investigates BM-CDRs, both theoretically and experimentally. We demonstrate two novel BM-CDR architectures. These BM-CDRs achieve error-free operation [bit error rate (BER) <10e--10] while providing instantaneous (0 preamble bit) clock phase acquisition for any phase step (+/-2pi rad) between successive bursts. Instantaneous phase acquisition improves the physical efficiency of upstream PON traffic, and increases the effective throughput of the system by raising the information rate. Our eloquent, scalable BM-CDR architectures leverage the design of low complexity commercial electronics providing a cost-effective solution for PONs. The first BM-CDR (rated at 5 Gb/s) is based on phase-tracking time domain oversampling (semiblind) CDR operated at 2x the bit rate and a clock phase aligner (CPA) that makes use of a phase picking algorithm. The second BM-CDR (rate at 10 Gb/s) is based on semiblind space domain oversampling and employs a phase-tracking CDR with multiphase clocks at the bit rate and a CPA with a novel phase picking algorithm. We experimentally demonstrate these BM-CDRs in optical test beds and study the effect of channel-impairments in: (1) 5 Gb/s time-division multiplexing gigabit PON 20-km uplink; (2) 2.5 Gb/s overlapped subcarrier-multiplexing wavelength-division multiplexed PON 20-km uplink; (3) 1.25 Gb/s 1300-km deployed fiber link spanning Montreal--Quebec City and back; and (4) 622 Mb/s in a 7-user spectral amplitude-coded optical code-division multiple access 20-km PON uplink. We also provide a theoretical framework to model and analyze BM-CDRs. We develop a unified probabilistic theory of BM-CDRs based on semiblind oversampling techniques in either the time or space domain. This theory has also been generalized for conventional CDRs and Nx-oversampling CDRs. Based on this theory, we perform a comprehensive theoretical analysis to quantify the performance of the proposed BM-CDRs in terms of the BER and packet loss ratio to assess the tradeoffs between various parameters, and compare the results experimentally to validate the theoretical model. This analysis coupled with the experimental results will refine theoretical models PONs, and provide input for establishing realistic power budgets.

Transmission over UWB channels with OFDM system using LDPC coding

NASA Astrophysics Data System (ADS)

Dziwoki, Grzegorz; Kucharczyk, Marcin; Sulek, Wojciech

2009-06-01

Hostile wireless environment requires use of sophisticated signal processing methods. The paper concerns on Ultra Wideband (UWB) transmission over Personal Area Networks (PAN) including MB-OFDM specification of physical layer. In presented work the transmission system with OFDM modulation was connected with LDPC encoder/decoder. Additionally the frame and bit error rate (FER and BER) of the system was decreased using results from the LDPC decoder in a kind of turbo equalization algorithm for better channel estimation. Computational block using evolutionary strategy, from genetic algorithms family, was also used in presented system. It was placed after SPA (Sum-Product Algorithm) decoder and is conditionally turned on in the decoding process. The result is increased effectiveness of the whole system, especially lower FER. The system was tested with two types of LDPC codes, depending on type of parity check matrices: randomly generated and constructed deterministically, optimized for practical decoder architecture implemented in the FPGA device.

A hybrid lightwave transmission system based on light injection/optoelectronic feedback techniques and fiber-VLLC integration

NASA Astrophysics Data System (ADS)

Tsai, Wen-Shing; Lu, Hai-Han; Li, Chung-Yi; Chen, Bo-Rui; Lin, Hung-Hsien; Lin, Dai-Hua

2016-04-01

A hybrid lightwave transmission system based on light injection/optoelectronic feedback techniques and fiber-visible laser light communication (VLLC) integration is proposed and experimentally demonstrated. To be the first one of its kind in employing light injection and optoelectronic feedback techniques in a fiber-VLLC integration lightwave transmission system, the light is successfully directly modulated with Community Access Television (CATV), 16-QAM, and 16-QAM-OFDM signals. Over a 40 km SMF and a 10 m free-space VLLC transport, good performances of carrier-to-noise ratio (CNR)/composite second-order (CSO)/composite triple-beat (CTB)/bit error rate (BER) are achieved for CATV/16-QAM/16-QAM-OFDM signals transmission. Such a hybrid lightwave transmission system would be very useful since it can provide broadband integrated services including CATV, Internet, and telecommunication services over both distribute fiber and in-building networks.

Atmospheric free-space coherent optical communications with adaptive optics

NASA Astrophysics Data System (ADS)

Ting, Chueh; Zhang, Chengyu; Yang, Zikai

2017-02-01

Free-space coherent optical communications have a potential application to offer last mile bottleneck solution in future local area networks (LAN) because of their information carrier, information security and license-free status. Coherent optical communication systems using orthogonal frequency division multiplexing (OFDM) digital modulation are successfully demonstrated in a long-haul tens Giga bits via optical fiber, but they are not yet available in free space due to atmospheric turbulence-induced channel fading. Adaptive optics is recognized as a promising technology to mitigate the effects of atmospheric turbulence in free-space optics. In this paper, a free-space coherent optical communication system using an OFDM digital modulation scheme and adaptive optics (FSO OFDM AO) is proposed, a Gamma-Gamma distribution statistical channel fading model for the FSO OFDM AO system is examined, and FSO OFDM AO system performance is evaluated in terms of bit error rate (BER) versus various propagation distances.

Progress and recent developments in the GAINS program

NASA Astrophysics Data System (ADS)

Girz, C. M. I. R.:; MacDonald, A. E.; Caracena, F.; Collander, R. S.; Jamison, B. D.; Anderson, R. L.; Latsch, D.; Lachenmeier, T.; Moody, R. A.; Mares, S.; Cooper, J.; Ganoe, G.; Katzberg, S.; Johnson, T.; Russ, B.

2001-08-01

The GAINS (Global Air-ocean IN-situ System) network of long-duration, high-altitude vehicles is proposed as a means to provide critically needed in-situ observations worldwide. This need is increasingly apparent, for example, in the Arctic where there is growing concern around the shrinking of the ice cap and sea ice extent with concomitant decreases in habitat for animal and plant species. In the mid-latitudes, the sustainability of sufficient soil moisture in grain producing regions is questionable under several climate change scenarios. Preparatory steps using smaller balloons and prototype payloads have been taken toward demonstrating the GAINS balloon concept. The balloon envelope recovery system (BERS) has been tested and radio frequency interference, compatibility and distance checks of the prototype command and communication systems were performed. Electronic and mechanical systems have been integrated in preparation for a 48-h flight of an 18-m diameter prototype.

The Community Science Workshop Network Story: Becoming a Networked Organization

ERIC Educational Resources Information Center

St. John, Mark

2014-01-01

The Community Science Workshops (CSWs)--with funding from the S.D. Bechtel, Jr. Foundation, and the Gordon and Betty Moore Foundation--created a network among the CSW sites in California. The goals of the CSW Network project have been to improve programs, build capacity throughout the Network, and establish new sites. Inverness Research has been…

Network Science Center Research Team’s Visit to Addis Ababa, Ethiopia

DTIC Science & Technology

2012-08-01

www.netscience.usma.edu 845.938.0804 enterprise that supports the German Government in achieving its objectives in the field of international cooperation for...U.S. Government . 14. ABSTRACT A Network Science Center research team demonstrated a network analysis “tool kit” to the Political and Economic...by China State Construction Engineering 3 | P a g e Network Science Center, West Point www.netscience.usma.edu 845.938.0804 Corporation as a

450-nm GaN laser diode enables high-speed visible light communication with 9-Gbps QAM-OFDM.

PubMed

Chi, Yu-Chieh; Hsieh, Dan-Hua; Tsai, Cheng-Ting; Chen, Hsiang-Yu; Kuo, Hao-Chung; Lin, Gong-Ru

2015-05-18

A TO-38-can packaged Gallium nitride (GaN) blue laser diode (LD) based free-space visible light communication (VLC) with 64-quadrature amplitude modulation (QAM) and 32-subcarrier orthogonal frequency division multiplexing (OFDM) transmission at 9 Gbps is preliminarily demonstrated over a 5-m free-space link. The 3-dB analog modulation bandwidth of the TO-38-can packaged GaN blue LD biased at 65 mA and controlled at 25°C is only 900 MHz, which can be extended to 1.5 GHz for OFDM encoding after throughput intensity optimization. When delivering the 4-Gbps 16-QAM OFDM data within 1-GHz bandwidth, the error vector magnitude (EVM), signal-to-noise ratio (SNR) and bit-error-rate (BER) of the received data are observed as 8.4%, 22.4 dB and 3.5 × 10(-8), respectively. By increasing the encoded bandwidth to 1.5 GHz, the TO-38-can packaged GaN blue LD enlarges its transmission capacity to 6 Gbps but degrades its transmitted BER to 1.7 × 10(-3). The same transmission capacity of 6 Gbps can also be achieved with a BER of 1 × 10(-6) by encoding 64-QAM OFDM data within 1-GHz bandwidth. Using the 1.5-GHz full bandwidth of the TO-38-can packaged GaN blue LD provides the 64-QAM OFDM transmission up to 9 Gbps, which successfully delivers data with an EVM of 5.1%, an SNR of 22 dB and a BER of 3.6 × 10(-3) passed the forward error correction (FEC) criterion.

Toward rapid analysis, forecast and discovery of bioactive compounds from herbs by jointly using thin layer chromatography and ratiometric surface-enhanced Raman spectroscopy technique.

PubMed

Gu, Xiaoling; Jin, Yang; Dong, Fang; Cai, Yueqing; You, Zhengyi; You, Junhui; Zhang, Liying; Du, Shuhu

2018-05-10

Conventional isolation and identification of active compounds from herbs have been extensively reported by using various chromatographic and spectroscopic techniques. However, how to quickly discover new bioactive ingredients from natural sources still remains a challenging task due to the interference of their similar structures or matrices. Here, we present a grand approach for rapid analysis, forecast and discovery of bioactive compounds from herbs based on a hyphenated strategy of thin layer chromatography and ratiometric surface-enhanced Raman spectroscopy. The performance of the hyphenated strategy is first evaluated by analyzing four protoberberine alkaloids, berberine (BER), coptisine (COP), palmatine (PAT) and jatrorrhizine (JAT), from a typical herb Coptidis Rhizoma as an example. It has been demonstrated that this coupling method can identify the four compounds by characteristic peaks at 728, 708, 736 and 732 cm -1 , and especially discriminate BER and COP (with similar migration distances) by ratiometric Raman intensity (I 708 /I 728 ). The corresponding limits of detection are 0.1, 0.05, 0.1 and 0.5 μM, respectively, which are about 1-2 orders of magnitude lower than those of direct observation method under 254 nm UV lamp. Based on these findings, the proposed method further guides forecast and discovery of unknown compounds from traditional Chinese herb Typhonii Rhizoma. Results infer that two trace alkaloids (BER and COP) from the n-butanol extract of Typhonii Rhizoma are found for the first time. Moreover, in vitro experiments manifest that BER can effectively decrease the viability of human glioma U87 cells by inducing cell cycle arrest in a concentration-dependent manner. Copyright © 2018 Elsevier B.V. All rights reserved.

Der Einfluss von Alitretinoin auf die Lebensqualität bei Patienten mit schwerem chronischen Handekzem: FUGETTA - Beobachtungsstudie unter Praxisbedingungen.

PubMed

Augustin, Matthias; Thaçi, Diamant; Kamps, Anja

2016-12-01

Alitretinoin ist die einzige zugelassene Behandlung für schweres chronisches Handekzem (CHE), das refraktär gegenüber potenten topischen Corticosteroiden ist. Bei dieser Studie (FUGETTA) wurde die Wirksamkeit von leitliniengerecht angewendetem oralem Alitretinoin sowie dessen Einfluss auf die Lebensqualität (LQ) bei Patienten mit schwerem refraktärem CHE beurteilt. Multizentrische, offene, nichtinterventionelle Beobachtungsstudie, durchgeführt in Deutschland. Die Patienten wurden nach Ermessen ihres behandelnden Arztes mit Alitretinoin 10 mg oder 30 mg einmal täglich über maximal 24 Wochen behandelt. Die Wirksamkeit wurde anhand des Physician Global Assessment (PGA) und des Dermatology Life Quality Index (DLQI) bewertet. Zudem wurden unerwünschte Ereignisse (UE) erfasst. Die Studienpopulation bestand aus 658 Patienten (30 mg: n = 581; 10 mg: n = 77). Bei Beobachtungsbeginn litten die meisten Patienten (83 %) gemäß PGA an einem schweren CHE. Bei Beobachtungsende war das Handekzem bei 48 % der Patienten gemäß PGA vollständig oder fast vollständig abgeheilt (30 mg: 49 %; 10 mg: 43 %). Die mittlere Verbesserung des DLQI-Scores in Woche 24 betrug 58 % (30 mg: mittlere [SD] Veränderung gegenüber dem Ausgangswert -10,4 [8,04]) und 70 % (10 mg: mittlere [SD] Veränderung gegenüber dem Ausgangswert -10,8 [7,29]). Die Gesamtinzidenz von NW war niedrig und in den beiden Gruppen ähnlich. Alitretinoin führte zu einer schnellen, deutlichen Verbesserung der LQ bei Patienten mit schwerem CHE. © 2016 Deutsche Dermatologische Gesellschaft (DDG). Published by John Wiley & Sons Ltd.

Role of base-excision repair in the treatment of childhood acute lymphoblastic leukaemia with 6-mercaptopurine and high doses of methotrexate.

PubMed

Stanczyk, M; Sliwinski, T; Trelinska, J; Cuchra, M; Markiewicz, L; Dziki, L; Bieniek, A; Bielecka-Kowalska, A; Kowalski, M; Pastorczak, A; Szemraj, J; Mlynarski, W; Majsterek, I

2012-01-24

Methotrexate (MTX) and 6-mercaptopurine (6MP) are the most commonly used drugs in the therapy of childhood acute lymphoblastic leukaemia (ALL). The main genotoxic effect of MTX resulting from inhibition of thymidylate synthase is mis-incorporation of uracil into DNA, which is considered essential for the effectiveness of the Protocol M in ALL IC BFM 2002/EURO LB 2002 regimens. In this study, we investigated the level of basal and induced DNA damage as well as the effectiveness of DNA repair in lymphocytes of children with ALL at four time-points during therapy with MTX and 6MP. To assess DNA damage and the efficacy of DNA repair we used the modified alkaline comet assay with uracil DNA glycosylase (Udg) and endonuclease III (EndoIII). In addition, we examined the induction of apoptosis in the lymphocytes of the patients during treatment. Finally, we compared the activity of base-excision repair (BER), involved in removal of both uracil and oxidized bases from DNA in lymphocytes of children with ALL and lymphocytes of healthy children. BER efficiency was estimated in an in vitro assay with cellular extracts and plasmid substrates of heteroduplex DNA with an AP-site. Our results indicate that there is a significant decrease in the efficacy of DNA repair associated with an increased level of uracil in DNA and induction of apoptosis during therapy. Moreover, it was found that the BER capacity was decreased in the lymphocytes of ALL patients in contrast to that in lymphocytes of healthy children. Thus, we suggest that an impairment of the BER pathway may play a role in the pathogenesis and therapy of childhood ALL. © 2011 Elsevier B.V. All rights reserved.

DNA Glycosylases Involved in Base Excision Repair May Be Associated with Cancer Risk in BRCA1 and BRCA2 Mutation Carriers

PubMed Central

Osorio, Ana; Milne, Roger L.; Kuchenbaecker, Karoline; Vaclová, Tereza; Pita, Guillermo; Alonso, Rosario; Peterlongo, Paolo; Blanco, Ignacio; de la Hoya, Miguel; Duran, Mercedes; Díez, Orland; Ramón y Cajal, Teresa; Konstantopoulou, Irene; Martínez-Bouzas, Cristina; Andrés Conejero, Raquel; Soucy, Penny; McGuffog, Lesley; Barrowdale, Daniel; Lee, Andrew; SWE-BRCA; Arver, Brita; Rantala, Johanna; Loman, Niklas; Ehrencrona, Hans; Olopade, Olufunmilayo I.; Beattie, Mary S.; Domchek, Susan M.; Nathanson, Katherine; Rebbeck, Timothy R.; Arun, Banu K.; Karlan, Beth Y.; Walsh, Christine; Lester, Jenny; John, Esther M.; Whittemore, Alice S.; Daly, Mary B.; Southey, Melissa; Hopper, John; Terry, Mary B.; Buys, Saundra S.; Janavicius, Ramunas; Dorfling, Cecilia M.; van Rensburg, Elizabeth J.; Steele, Linda; Neuhausen, Susan L.; Ding, Yuan Chun; Hansen, Thomas v. O.; Jønson, Lars; Ejlertsen, Bent; Gerdes, Anne-Marie; Infante, Mar; Herráez, Belén; Moreno, Leticia Thais; Weitzel, Jeffrey N.; Herzog, Josef; Weeman, Kisa; Manoukian, Siranoush; Peissel, Bernard; Zaffaroni, Daniela; Scuvera, Giulietta; Bonanni, Bernardo; Mariette, Frederique; Volorio, Sara; Viel, Alessandra; Varesco, Liliana; Papi, Laura; Ottini, Laura; Tibiletti, Maria Grazia; Radice, Paolo; Yannoukakos, Drakoulis; Garber, Judy; Ellis, Steve; Frost, Debra; Platte, Radka; Fineberg, Elena; Evans, Gareth; Lalloo, Fiona; Izatt, Louise; Eeles, Ros; Adlard, Julian; Davidson, Rosemarie; Cole, Trevor; Eccles, Diana; Cook, Jackie; Hodgson, Shirley; Brewer, Carole; Tischkowitz, Marc; Douglas, Fiona; Porteous, Mary; Side, Lucy; Walker, Lisa; Morrison, Patrick; Donaldson, Alan; Kennedy, John; Foo, Claire; Godwin, Andrew K.; Schmutzler, Rita Katharina; Wappenschmidt, Barbara; Rhiem, Kerstin; Engel, Christoph; Meindl, Alfons; Ditsch, Nina; Arnold, Norbert; Plendl, Hans Jörg; Niederacher, Dieter; Sutter, Christian; Wang-Gohrke, Shan; Steinemann, Doris; Preisler-Adams, Sabine; Kast, Karin; Varon-Mateeva, Raymonda; Gehrig, Andrea; Stoppa-Lyonnet, Dominique; Sinilnikova, Olga M.; Mazoyer, Sylvie; Damiola, Francesca; Poppe, Bruce; Claes, Kathleen; Piedmonte, Marion; Tucker, Kathy; Backes, Floor; Rodríguez, Gustavo; Brewster, Wendy; Wakeley, Katie; Rutherford, Thomas; Caldés, Trinidad; Nevanlinna, Heli; Aittomäki, Kristiina; Rookus, Matti A.; van Os, Theo A. M.; van der Kolk, Lizet; de Lange, J. L.; Meijers-Heijboer, Hanne E. J.; van der Hout, A. H.; van Asperen, Christi J.; Gómez Garcia, Encarna B.; Hoogerbrugge, Nicoline; Collée, J. Margriet; van Deurzen, Carolien H. M.; van der Luijt, Rob B.; Devilee, Peter; HEBON; Olah, Edith; Lázaro, Conxi; Teulé, Alex; Menéndez, Mireia; Jakubowska, Anna; Cybulski, Cezary; Gronwald, Jacek; Lubinski, Jan; Durda, Katarzyna; Jaworska-Bieniek, Katarzyna; Johannsson, Oskar Th.; Maugard, Christine; Montagna, Marco; Tognazzo, Silvia; Teixeira, Manuel R.; Healey, Sue; Investigators, kConFab; Olswold, Curtis; Guidugli, Lucia; Lindor, Noralane; Slager, Susan; Szabo, Csilla I.; Vijai, Joseph; Robson, Mark; Kauff, Noah; Zhang, Liying; Rau-Murthy, Rohini; Fink-Retter, Anneliese; Singer, Christian F.; Rappaport, Christine; Geschwantler Kaulich, Daphne; Pfeiler, Georg; Tea, Muy-Kheng; Berger, Andreas; Phelan, Catherine M.; Greene, Mark H.; Mai, Phuong L.; Lejbkowicz, Flavio; Andrulis, Irene; Mulligan, Anna Marie; Glendon, Gord; Toland, Amanda Ewart; Bojesen, Anders; Pedersen, Inge Sokilde; Sunde, Lone; Thomassen, Mads; Kruse, Torben A.; Jensen, Uffe Birk; Friedman, Eitan; Laitman, Yael; Shimon, Shani Paluch; Simard, Jacques; Easton, Douglas F.; Offit, Kenneth; Couch, Fergus J.; Chenevix-Trench, Georgia; Antoniou, Antonis C.; Benitez, Javier

2014-01-01

Single Nucleotide Polymorphisms (SNPs) in genes involved in the DNA Base Excision Repair (BER) pathway could be associated with cancer risk in carriers of mutations in the high-penetrance susceptibility genes BRCA1 and BRCA2, given the relation of synthetic lethality that exists between one of the components of the BER pathway, PARP1 (poly ADP ribose polymerase), and both BRCA1 and BRCA2. In the present study, we have performed a comprehensive analysis of 18 genes involved in BER using a tagging SNP approach in a large series of BRCA1 and BRCA2 mutation carriers. 144 SNPs were analyzed in a two stage study involving 23,463 carriers from the CIMBA consortium (the Consortium of Investigators of Modifiers of BRCA1 and BRCA2). Eleven SNPs showed evidence of association with breast and/or ovarian cancer at p<0.05 in the combined analysis. Four of the five genes for which strongest evidence of association was observed were DNA glycosylases. The strongest evidence was for rs1466785 in the NEIL2 (endonuclease VIII-like 2) gene (HR: 1.09, 95% CI (1.03–1.16), p = 2.7×10−3) for association with breast cancer risk in BRCA2 mutation carriers, and rs2304277 in the OGG1 (8-guanine DNA glycosylase) gene, with ovarian cancer risk in BRCA1 mutation carriers (HR: 1.12 95%CI: 1.03–1.21, p = 4.8×10−3). DNA glycosylases involved in the first steps of the BER pathway may be associated with cancer risk in BRCA1/2 mutation carriers and should be more comprehensively studied. PMID:24698998

Nuclear depletion of apurinic/apyrimidinic endonuclease 1 (Ape1/Ref-1) is an indicator of energy disruption in neurons.

PubMed

Singh, Shilpee; Englander, Ella W

2012-11-01

Apurinic/apyrimidinic endonuclease 1 (Ape1/Ref-1) is a multifunctional protein critical for cellular survival. Its involvement in adaptive survival responses includes key roles in redox sensing, transcriptional regulation, and repair of DNA damage via the base excision repair (BER) pathway. Ape1 is abundant in most cell types and central in integrating the first BER step catalyzed by different DNA glycosylases. BER is the main process for removal of oxidative DNA lesions in postmitotic brain cells, and after ischemic brain injury preservation of Ape1 coincides with neuronal survival, while its loss has been associated with neuronal death. Here, we report that in cultured primary neurons, diminution of cellular ATP by either oligomycin or H(2)O(2) is accompanied by depletion of nuclear Ape1, while other BER proteins are unaffected and retain their nuclear localization under these conditions. Importantly, while H(2)O(2) induces γH2AX phosphorylation, indicative of chromatin rearrangements in response to DNA damage, oligomycin does not. Furthermore, despite comparable diminution of ATP content, H(2)O(2) and oligomycin differentially affect critical parameters of mitochondrial respiration that ultimately determine cellular ATP content. Taken together, our findings demonstrate that in neurons, nuclear compartmentalization of Ape1 depends on ATP and loss of nuclear Ape1 reflects disruption of neuronal energy homeostasis. Energy crisis is a hallmark of stroke and other ischemic/hypoxic brain injuries. In vivo studies have shown that Ape1 deficit precedes neuronal loss in injured brain regions. Thus, our findings bring to light the possibility that energy failure-induced Ape1 depletion triggers neuronal death in ischemic brain injuries. Copyright © 2012 Elsevier Inc. All rights reserved.

Parp1 activation in mouse embryonic fibroblasts promotes Pol β-dependent cellular hypersensitivity to alkylation damage

PubMed Central

Jelezcova, Elena; Trivedi, Ram N.; Wang, Xiao-hong; Tang, Jiang-bo; Brown, Ashley R.; Goellner, Eva M.; Schamus, Sandy; Fornsaglio, Jamie L.; Sobol, Robert W.

2010-01-01

Alkylating agents induce cell death in wild-type (WT) mouse embryonic fibroblasts (MEFs) by multiple mechanisms, including apoptosis, autophagy and necrosis. DNA polymerase β (Pol β) knockout (KO) MEFs are hypersensitive to the cytotoxic effect of alkylating agents, as compared to WT MEFs. To test the hypothesis that Parp1 is preferentially activated by methyl methanesulfonate (MMS) exposure of Pol β KO MEFs, we have examined the relationship between Pol β expression, Parp1 activation and cell survival following MMS exposure in a series of WT and Pol β deficient MEF cell lines. Consistent with our hypothesis, we observed elevated Parp1 activation in Pol β KO MEFs as compared to matched WT MEFs. Both the MMS-induced activation of Parp1 and the MMS-induced cytoxicity of Pol β KO MEFs are attenuated by pre-treatment with the Parp1/Parp2 inhibitor PJ34. Further, elevated Parp1 activation is observed following knockdown (KD) of endogenous Pol β, as compared to WT cells. Pol β KD MEFs are hypersensitive to MMS and both the MMS-induced hypersensitivity and Parp1 activation is prevented by pre-treatment with PJ34. In addition, the MMS-induced cellular sensitivity of Pol β KO MEFs is reversed when Parp1 is also deleted (Pol β/Parp1 double KO MEFs) and we observe no MMS sensitivity differential between Pol β/Parp1 double KO MEFs and those that express recombinant mouse Pol β. These studies suggest that Parp1 may function as a sensor of BER to initiate cell death when BER is aborted or fails. Parp1 may therefore function in BER as a tumor suppressor by initiating cell death and preventing the accumulation of cells with chromosomal damage due to a BER defect. PMID:20096707

Foundations of data-intensive science: Technology and practice for high throughput, widely distributed, data management and analysis systems

NASA Astrophysics Data System (ADS)

Johnston, William; Ernst, M.; Dart, E.; Tierney, B.

2014-04-01

Today's large-scale science projects involve world-wide collaborations depend on moving massive amounts of data from an instrument to potentially thousands of computing and storage systems at hundreds of collaborating institutions to accomplish their science. This is true for ATLAS and CMS at the LHC, and it is true for the climate sciences, Belle-II at the KEK collider, genome sciences, the SKA radio telescope, and ITER, the international fusion energy experiment. DOE's Office of Science has been collecting science discipline and instrument requirements for network based data management and analysis for more than a decade. As a result of this certain key issues are seen across essentially all science disciplines that rely on the network for significant data transfer, even if the data quantities are modest compared to projects like the LHC experiments. These issues are what this talk will address; to wit: 1. Optical signal transport advances enabling 100 Gb/s circuits that span the globe on optical fiber with each carrying 100 such channels; 2. Network router and switch requirements to support high-speed international data transfer; 3. Data transport (TCP is still the norm) requirements to support high-speed international data transfer (e.g. error-free transmission); 4. Network monitoring and testing techniques and infrastructure to maintain the required error-free operation of the many R&E networks involved in international collaborations; 5. Operating system evolution to support very high-speed network I/O; 6. New network architectures and services in the LAN (campus) and WAN networks to support data-intensive science; 7. Data movement and management techniques and software that can maximize the throughput on the network connections between distributed data handling systems, and; 8. New approaches to widely distributed workflow systems that can support the data movement and analysis required by the science. All of these areas must be addressed to enable large-scale, widely distributed data analysis systems, and the experience of the LHC can be applied to other scientific disciplines. In particular, specific analogies to the SKA will be cited in the talk.

ESnet: Large-Scale Science and Data Management ( (LBNL Summer Lecture Series)

ScienceCinema

Johnston, Bill

2017-12-09

Summer Lecture Series 2004: Bill Johnston of Berkeley Lab's Computing Sciences is a distinguished networking and computing researcher. He managed the Energy Sciences Network (ESnet), a leading-edge, high-bandwidth network funded by DOE's Office of Science. Used for everything from videoconferencing to climate modeling, and flexible enough to accommodate a wide variety of data-intensive applications and services, ESNet's traffic volume is doubling every year and currently surpasses 200 terabytes per month.

Data-Driven Design of Intelligent Wireless Networks: An Overview and Tutorial.

PubMed

Kulin, Merima; Fortuna, Carolina; De Poorter, Eli; Deschrijver, Dirk; Moerman, Ingrid

2016-06-01

Data science or "data-driven research" is a research approach that uses real-life data to gain insight about the behavior of systems. It enables the analysis of small, simple as well as large and more complex systems in order to assess whether they function according to the intended design and as seen in simulation. Data science approaches have been successfully applied to analyze networked interactions in several research areas such as large-scale social networks, advanced business and healthcare processes. Wireless networks can exhibit unpredictable interactions between algorithms from multiple protocol layers, interactions between multiple devices, and hardware specific influences. These interactions can lead to a difference between real-world functioning and design time functioning. Data science methods can help to detect the actual behavior and possibly help to correct it. Data science is increasingly used in wireless research. To support data-driven research in wireless networks, this paper illustrates the step-by-step methodology that has to be applied to extract knowledge from raw data traces. To this end, the paper (i) clarifies when, why and how to use data science in wireless network research; (ii) provides a generic framework for applying data science in wireless networks; (iii) gives an overview of existing research papers that utilized data science approaches in wireless networks; (iv) illustrates the overall knowledge discovery process through an extensive example in which device types are identified based on their traffic patterns; (v) provides the reader the necessary datasets and scripts to go through the tutorial steps themselves.

Data-Driven Design of Intelligent Wireless Networks: An Overview and Tutorial

PubMed Central

Kulin, Merima; Fortuna, Carolina; De Poorter, Eli; Deschrijver, Dirk; Moerman, Ingrid

2016-01-01

Data science or “data-driven research” is a research approach that uses real-life data to gain insight about the behavior of systems. It enables the analysis of small, simple as well as large and more complex systems in order to assess whether they function according to the intended design and as seen in simulation. Data science approaches have been successfully applied to analyze networked interactions in several research areas such as large-scale social networks, advanced business and healthcare processes. Wireless networks can exhibit unpredictable interactions between algorithms from multiple protocol layers, interactions between multiple devices, and hardware specific influences. These interactions can lead to a difference between real-world functioning and design time functioning. Data science methods can help to detect the actual behavior and possibly help to correct it. Data science is increasingly used in wireless research. To support data-driven research in wireless networks, this paper illustrates the step-by-step methodology that has to be applied to extract knowledge from raw data traces. To this end, the paper (i) clarifies when, why and how to use data science in wireless network research; (ii) provides a generic framework for applying data science in wireless networks; (iii) gives an overview of existing research papers that utilized data science approaches in wireless networks; (iv) illustrates the overall knowledge discovery process through an extensive example in which device types are identified based on their traffic patterns; (v) provides the reader the necessary datasets and scripts to go through the tutorial steps themselves. PMID:27258286

Can multilayer brain networks be a real step forward?. Comment on "Network science of biological systems at different scales: A review" by M. Gosak et al.

NASA Astrophysics Data System (ADS)

Buldú, Javier M.; Papo, David

2018-03-01

Over the last two decades Network Science has become one of the most active fields in science, whose growth has been supported by four fundamental pillars: statistical physics, nonlinear dynamics, graph theory and Big Data [1]. Initially concerned with analyzing the structure of networks, Network Science rapidly turned its attention, focused on the implications of network topology, on the dynamics of and processes unfolding on networked systems, greatly improving our understanding of diffusion, synchronization, epidemics and information transmission in complex systems [2]. The network approach typically considered complex systems as evolving in a vacuum; however real networks are generally not isolated systems, but are in continuous and evolving contact with other networks, with which they interact in multiple qualitative different and typically time-varying ways. These systems can then be represented as a collection of subsystems with connectivity layers, which are simply collapsed when considering the traditional monolayer representation. Surprisingly, such an "unpacking" of layers has proven to bear profound consequences on the structural and dynamical properties of networks, leading for instance to counter-intuitive synchronization phenomena, where maximization synchronization is achieved through strategies opposite of those maximizing synchronization in isolated networks [3].

The Intellectual Structure of Research on Educational Technology in Science Education (ETiSE): A Co-citation Network Analysis of Publications in Selected Journals (2008-2013)

NASA Astrophysics Data System (ADS)

Tang, Kai-Yu; Tsai, Chin-Chung

2016-01-01

The main purpose of this paper is to investigate the intellectual structure of the research on educational technology in science education (ETiSE) within the most recent years (2008-2013). Based on the criteria for educational technology research and the citation threshold for educational co-citation analysis, a total of 137 relevant ETiSE papers were identified from the International Journal of Science Education, the Journal of Research in Science Teaching, Science Education, and the Journal of Science Education and Technology. Then, a series of methodologies were performed to analyze all 137 source documents, including document co-citation analysis, social network analysis, and exploratory factor analysis. As a result, 454 co-citation ties were obtained and then graphically visualized with an undirected network, presenting a global structure of the current ETiSE research network. In addition, four major underlying intellectual subfields within the main component of the ETiSE network were extracted and named as: (1) technology-enhanced science inquiry, (2) simulation and visualization for understanding, (3) technology-enhanced chemistry learning, and (4) game-based science learning. The most influential co-citation pairs and cross-boundary phenomena were then analyzed and visualized in a co-citation network. This is the very first attempt to illuminate the core ideas underlying ETiSE research by integrating the co-citation method, factor analysis, and the networking visualization technique. The findings of this study provide a platform for scholarly discussion of the dissemination and research trends within the current ETiSE literature.

Communications among data and science centers

NASA Technical Reports Server (NTRS)

Green, James L.

1990-01-01

The ability to electronically access and query the contents of remote computer archives is of singular importance in space and earth sciences; the present evaluation of such on-line information networks' development status foresees swift expansion of their data capabilities and complexity, in view of the volumes of data that will continue to be generated by NASA missions. The U.S.'s National Space Science Data Center (NSSDC) manages NASA's largest science computer network, the Space Physics Analysis Network; a comprehensive account is given of the structure of NSSDC international access through BITNET, and of connections to the NSSDC available in the Americas via the International X.25 network.

Practical use of a framework for network science experimentation

NASA Astrophysics Data System (ADS)

Toth, Andrew; Bergamaschi, Flavio

2014-06-01

In 2006, the US Army Research Laboratory (ARL) and the UK Ministry of Defence (MoD) established a collaborative research alliance with academia and industry, called the International Technology Alliance (ITA)1 In Network and Information Sciences, to address fundamental issues concerning Network and Information Sciences that will enhance decision making for coalition operations and enable rapid, secure formation of ad hoc teams in coalition environments and enhance US and UK capabilities to conduct coalition warfare. Research conducted under the ITA was extended through collaboration between ARL and IBM UK to characterize and dene a software stack and tooling that has become the reference framework for network science experimentation in support for validation of theoretical research. This paper discusses the composition of the reference framework for experimentation resulting from the ARL/IBM UK collaboration and its use, by the Network Science Collaborative Technology Alliance (NS CTA)2 , in a recent network science experiment conducted at ARL. It also discusses how the experiment was modeled using the reference framework, the integration of two new components, the Apollo Fact-Finder3 tool and the Medusa Crowd Sensing4 application, the limitations identified and how they shall be addressed in future work.

The Role of Informal Support Networks in Teaching the Nature of Science

NASA Astrophysics Data System (ADS)

Herman, Benjamin C.; Olson, Joanne K.; Clough, Michael P.

2017-06-01

This study reports the participation of 13 secondary science teachers in informal support networks and how that participation was associated with their nature of science (NOS) teaching practices 2 to 5 years after having graduated from the same science teacher education program. The nine teachers who participated in informal support networks taught the NOS at high/medium levels, while the four non-participating teachers taught the NOS at low levels. The nine high/medium NOS implementation teachers credited the informal support networks for maintaining/heightening their sense of responsibility for teaching NOS and for helping them navigate institutional constraints that impede effective NOS instruction. Several high/medium NOS instruction implementers initially struggled to autonomously frame and resolve the complexities experienced in schools and thus drew from the support networks to engage in more sophisticated forms of teacher decision-making. In contrast, the NOS pedagogical decisions of the four teachers not participating in support networks were governed primarily by the expectations and constraints experienced in their schools. Implications of this study include the need for reconsidering the structure of teacher mentorship programs to ensure they do not promote archaic science teaching practices that are at odds with reform efforts in science education.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tierney, Brian; Dart, Eli; Tierney, Brian

The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy Office of Science, the single largest supporter of basic research in the physical sciences in the United States of America. In support of the Office of Science programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 20 years. In March 2008, ESnet and the Fusion Energy Sciences (FES) Program Office of themore » DOE Office of Science organized a workshop to characterize the networking requirements of the science programs funded by the FES Program Office. Most sites that conduct data-intensive activities (the Tokamaks at GA and MIT, the supercomputer centers at NERSC and ORNL) show a need for on the order of 10 Gbps of network bandwidth for FES-related work within 5 years. PPPL reported a need for 8 times that (80 Gbps) in that time frame. Estimates for the 5-10 year time period are up to 160 Mbps for large simulations. Bandwidth requirements for ITER range from 10 to 80 Gbps. In terms of science process and collaboration structure, it is clear that the proposed Fusion Simulation Project (FSP) has the potential to significantly impact the data movement patterns and therefore the network requirements for U.S. fusion science. As the FSP is defined over the next two years, these changes will become clearer. Also, there is a clear and present unmet need for better network connectivity between U.S. FES sites and two Asian fusion experiments--the EAST Tokamak in China and the KSTAR Tokamak in South Korea. In addition to achieving its goal of collecting and characterizing the network requirements of the science endeavors funded by the FES Program Office, the workshop emphasized that there is a need for research into better ways of conducting remote collaboration with the control room of a Tokamak running an experiment. This is especially important since the current plans for ITER assume that this problem will be solved.« less

A Space Operations Network Alternative: Using Globally Connected Research and Education Networks for Space-Based Science Operations

NASA Technical Reports Server (NTRS)

Bradford, Robert N.

2006-01-01

Earth based networking in support of various space agency projects has been based on leased service/circuits which has a high associated cost. This cost is almost always taken from the science side resulting in less science. This is a proposal to use Research and Education Networks (RENs) worldwide to support space flight operations in general and space-based science operations in particular. The RENs were developed to support scientific and educational endeavors. They do not provide support for general Internet traffic. The connectivity and performance of the research and education networks is superb. The connectivity at Layer 3 (IP) virtually encompasses the globe. Most third world countries and all developed countries have their own research and education networks, which are connected globally. Performance of the RENs especially in the developed countries is exceptional. Bandwidth capacity currently exists and future expansion promises that this capacity will continue. REN performance statistics has always exceeded minimum requirements for spaceflight support. Research and Education networks are more loosely managed than a corporate network but are highly managed when compared to the commodity Internet. Management of RENs on an international level is accomplished by the International Network Operations Center at Indiana University at Indianapolis. With few exceptions, each regional and national REN has its own network ops center. The acceptable use policies (AUP), although differing by country, allows any scientific program or project the use of their networks. Once in compliance with the first RENs AUP, all others will accept that specific traffic including regional and transoceanic networks. RENs can support spaceflight related scientific programs and projects. Getting the science to the researcher is obviously key to any scientific project. RENs provide a pathway to virtually any college or university in the world, as well as many governmental institutes and science centers. RENs are not to be used for mission critical types of network traffic, even though RENs performance characteristics would support it.

Code 672 observational science branch computer networks

NASA Technical Reports Server (NTRS)

Hancock, D. W.; Shirk, H. G.

1988-01-01

In general, networking increases productivity due to the speed of transmission, easy access to remote computers, ability to share files, and increased availability of peripherals. Two different networks within the Observational Science Branch are described in detail.

Advanced Scientific Computing Research Network Requirements: ASCR Network Requirements Review Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bacon, Charles; Bell, Greg; Canon, Shane

The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy (DOE) Office of Science (SC), the single largest supporter of basic research in the physical sciences in the United States. In support of SC programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 25 years. In October 2012, ESnet and the Office of Advanced Scientific Computing Research (ASCR) of the DOE SCmore » organized a review to characterize the networking requirements of the programs funded by the ASCR program office. The requirements identified at the review are summarized in the Findings section, and are described in more detail in the body of the report.« less

NSI operations center

NASA Technical Reports Server (NTRS)

Zanley, Nancy L.

1991-01-01

The NASA Science Internet (NSI) Network Operations Staff is responsible for providing reliable communication connectivity for the NASA science community. As the NSI user community expands, so does the demand for greater interoperability with users and resources on other networks (e.g., NSFnet, ESnet), both nationally and internationally. Coupled with the science community's demand for greater access to other resources is the demand for more reliable communication connectivity. Recognizing this, the NASA Science Internet Project Office (NSIPO) expands its Operations activities. By January 1990, Network Operations was equipped with a telephone hotline, and its staff was expanded to six Network Operations Analysts. These six analysts provide 24-hour-a-day, 7-day-a-week coverage to assist site managers with problem determination and resolution. The NSI Operations staff monitors network circuits and their associated routers. In most instances, NSI Operations diagnoses and reports problems before users realize a problem exists. Monitoring of the NSI TCP/IP Network is currently being done with Proteon's Overview monitoring system. The Overview monitoring system displays a map of the NSI network utilizing various colors to indicate the conditions of the components being monitored. Each node or site is polled via the Simple Network Monitoring Protocol (SNMP). If a circuit goes down, Overview alerts the Network Operations staff with an audible alarm and changes the color of the component. When an alert is received, Network Operations personnel immediately verify and diagnose the problem, coordinate repair with other networking service groups, track problems, and document problem and resolution into a trouble ticket data base. NSI Operations offers the NSI science community reliable connectivity by exercising prompt assessment and resolution of network problems.

Alternative Antriebe für Automobile: Hybridsysteme, Brennstoffzellen, alternative Energieträger

NASA Astrophysics Data System (ADS)

Stan, Cornel

Über die Realisierungsmöglichkeiten zukünftiger Antriebskonzepte - von Hybridsystemen Elektro-/Verbrennungsmotor über Brennstoffzellen bis zu alternativen Energieträgern wie Wasserstoff oder Alkohol - werden fundierte Kriterien der Qualität eines Antriebs entscheiden. Leistungsdichte, Drehmomentverlauf, Beschleunigungscharakteristik, spezifischer Energieverbrauch sowie Emission chemischer Stoffe und Geräusche sind dafür wichtige Merkmale zur Qualitätsbeurteilung. Die Verfügbarkeit und die Speicherfähigkeit vorgesehener Energieträger, die technische Komplexität, Kosten, Sicherheit, Infrastruktur und Service werden die Randbedingungen für die Einführung realisierbarer Konzepte alternativer Antriebe für Automobile stellen.

Amerikas Einschätzung der deutschen Atomforschung: Das deutsche Uranprojekt

NASA Astrophysics Data System (ADS)

Walker, Mark

2002-07-01

Die amerikanischen Wissenschaftler und ihre emigrierten Kollegen, die am Bau der Atombombe beteiligt waren, verfügten über sehr widersprüchliche und großteils falsche Informationen über den Fortschritt des deutschen Uranprogramms. Noch nach Kriegsende lässt sich dies an Aussagen des Leiters der amerikanischen Alsos-Mission, Samuel Goudsmit, festmachen. Tatsächlich war das deutsche Programm hinsichtlich seiner wissenschaftlichen Grundlagen und des Managements nicht so unterlegen, wie vielfach behauptet wurde. Aber die deutschen Behörden waren nicht in der Lage, Geld und Ressourcen in gleichem Maße in das Uranprojekt zu investieren, wie etwa in das Peenemünder Raketenprojekt.

Fiber optical sensor system for shape and haptics for flexible instruments in minimally invasive surgery: overview and status quo

NASA Astrophysics Data System (ADS)

Ledermann, Christoph; Pauer, Hendrikje; Woern, Heinz

2014-05-01

In minimally invasive surgery, exible mechatronic instruments promise to improve the overall performance of surgical interventions. However, those instruments require highly developed sensors in order to provide haptic feedback to the surgeon or to enable (semi-)autonomous tasks. Precisely, haptic sensors and a shape sensor are required. In this paper, we present our ber optical sensor system of Fiber Bragg Gratings, which consists of a shape sensor, a kinesthetic sensor and a tactile sensor. The status quo of each of the three sensors is described, as well as the concept to integrate them into one ber optical sensor system.

Applying EVM to Satellite on Ground and In-Orbit Testing - Better Data in Less Time

NASA Technical Reports Server (NTRS)

Peters, Robert; Lebbink, Elizabeth-Klein; Lee, Victor; Model, Josh; Wezalis, Robert; Taylor, John

2008-01-01

Using Error Vector Magnitude (EVM) in satellite integration and test allows rapid verification of the Bit Error Rate (BER) performance of a satellite link and is particularly well suited to measurement of low bit rate satellite links where it can result in a major reduction in test time (about 3 weeks per satellite for the Geosynchronous Operational Environmental Satellite [GOES] satellites during ground test) and can provide diagnostic information. Empirical techniques developed to predict BER performance from EVM measurements and lessons learned about applying these techniques during GOES N, O, and P integration test and post launch testing, are discussed.

On the reduced-complexity of LDPC decoders for ultra-high-speed optical transmission.

PubMed

Djordjevic, Ivan B; Xu, Lei; Wang, Ting

2010-10-25

We propose two reduced-complexity (RC) LDPC decoders, which can be used in combination with large-girth LDPC codes to enable ultra-high-speed serial optical transmission. We show that optimally attenuated RC min-sum sum algorithm performs only 0.46 dB (at BER of 10(-9)) worse than conventional sum-product algorithm, while having lower storage memory requirements and much lower latency. We further study the use of RC LDPC decoding algorithms in multilevel coded modulation with coherent detection and show that with RC decoding algorithms we can achieve the net coding gain larger than 11 dB at BERs below 10(-9).

XRCC1 suppresses somatic hypermutation and promotes alternative nonhomologous end joining in Igh genes.

PubMed

Saribasak, Huseyin; Maul, Robert W; Cao, Zheng; McClure, Rhonda L; Yang, William; McNeill, Daniel R; Wilson, David M; Gearhart, Patricia J

2011-10-24

Activation-induced deaminase (AID) deaminates cytosine to uracil in immunoglobulin genes. Uracils in DNA can be recognized by uracil DNA glycosylase and abasic endonuclease to produce single-strand breaks. The breaks are repaired either faithfully by DNA base excision repair (BER) or mutagenically to produce somatic hypermutation (SHM) and class switch recombination (CSR). To unravel the interplay between repair and mutagenesis, we decreased the level of x-ray cross-complementing 1 (XRCC1), a scaffold protein involved in BER. Mice heterozygous for XRCC1 showed a significant increase in the frequencies of SHM in Igh variable regions in Peyer's patch cells, and of double-strand breaks in the switch regions during CSR. Although the frequency of CSR was normal in Xrcc1(+/-) splenic B cells, the length of microhomology at the switch junctions decreased, suggesting that XRCC1 also participates in alternative nonhomologous end joining. Furthermore, Xrcc1(+/-) B cells had reduced Igh/c-myc translocations during CSR, supporting a role for XRCC1 in microhomology-mediated joining. Our results imply that AID-induced single-strand breaks in Igh variable and switch regions become substrates simultaneously for BER and mutagenesis pathways.

Low Temperature Plasma Kills SCaBER Cancer Cells

NASA Astrophysics Data System (ADS)

Barekzi, Nazir; van Way, Lucas; Laroussi, Mounir

2013-09-01

Squamous cell carcinoma of the bladder is a rare type of bladder cancer that forms as a result of chronic irritation of the epithelial lining of the bladder. The cell line used in this study is SCaBER (ATCC® HTB-3™) derived from squamous cell carcinoma of the human urinary bladder. Current treatments of bladder cancer include surgery, radiation and chemotherapy. However, the cost of these treatments, the potential toxicity of the chemotherapeutic agents and the systemic side-effects warrant an alternative to current cancer treatment. This paper represents preliminary studies to determine the effects of biologically tolerant plasma (BTP) on a cell line of human bladder cancer cells. Previous work by our group using the plasma pencil revealed the efficacy of BTP on leukemia cells suspended in solution. Based on these earlier findings we hypothesized that the plasma exposure would elicit a similar programmed cell death in the SCaBER cells. Trypan blue exclusion and MTT assays revealed the cell killing after exposure to BTP. Our study indicates that low temperature plasma generated by ionizing helium gas and the reactive species may be a suitable and safe alternative for cancer therapy.

Nonlinear Demodulation and Channel Coding in EBPSK Scheme

PubMed Central

Chen, Xianqing; Wu, Lenan

2012-01-01

The extended binary phase shift keying (EBPSK) is an efficient modulation technique, and a special impacting filter (SIF) is used in its demodulator to improve the bit error rate (BER) performance. However, the conventional threshold decision cannot achieve the optimum performance, and the SIF brings more difficulty in obtaining the posterior probability for LDPC decoding. In this paper, we concentrate not only on reducing the BER of demodulation, but also on providing accurate posterior probability estimates (PPEs). A new approach for the nonlinear demodulation based on the support vector machine (SVM) classifier is introduced. The SVM method which selects only a few sampling points from the filter output was used for getting PPEs. The simulation results show that the accurate posterior probability can be obtained with this method and the BER performance can be improved significantly by applying LDPC codes. Moreover, we analyzed the effect of getting the posterior probability with different methods and different sampling rates. We show that there are more advantages of the SVM method under bad condition and it is less sensitive to the sampling rate than other methods. Thus, SVM is an effective method for EBPSK demodulation and getting posterior probability for LDPC decoding. PMID:23213281

A novel regulatory circuit in base excision repair involving AP endonuclease 1, Creb1 and DNA polymerase β

PubMed Central

Pei, De-Sheng; Yang, Xiao-Jie; Liu, Wei; Guikema, Jeroen E. J.; Schrader, Carol E.; Strauss, Phyllis R.

2011-01-01

DNA repair is required to maintain genome stability in stem cells and early embryos. At critical junctures, oxidative damage to DNA requires the base excision repair (BER) pathway. Since early zebrafish embryos lack the major polymerase in BER, DNA polymerase ß, repair proceeds via replicative polymerases, even though there is ample polb mRNA. Here, we report that Polb protein fails to appear at the appropriate time in development when AP endonuclease 1 (Apex), the upstream protein in BER, is knocked down. Because polb contains a Creb1 binding site, we examined whether knockdown of Apex affects creb1. Apex knockdown results in loss of Creb1 and Creb complex members but not Creb1 phosphorylation. This effect is independent of p53. Although both apex and creb1 mRNA rescue Creb1 and Polb after Apex knockdown, Apex is not a co-activator of creb1 transcription. This observation has broad significance, as similar results occur when Apex is inhibited in B cells from apex+/− mice. These results describe a novel regulatory circuit involving Apex, Creb1 and Polb and provide a mechanism for lethality of Apex loss in higher eukaryotes. PMID:21172930

A negentropy minimization approach to adaptive equalization for digital communication systems.

PubMed

Choi, Sooyong; Lee, Te-Won

2004-07-01

In this paper, we introduce and investigate a new adaptive equalization method based on minimizing approximate negentropy of the estimation error for a finite-length equalizer. We consider an approximate negentropy using nonpolynomial expansions of the estimation error as a new performance criterion to improve performance of a linear equalizer based on minimizing minimum mean squared error (MMSE). Negentropy includes higher order statistical information and its minimization provides improved converge, performance and accuracy compared to traditional methods such as MMSE in terms of bit error rate (BER). The proposed negentropy minimization (NEGMIN) equalizer has two kinds of solutions, the MMSE solution and the other one, depending on the ratio of the normalization parameters. The NEGMIN equalizer has best BER performance when the ratio of the normalization parameters is properly adjusted to maximize the output power(variance) of the NEGMIN equalizer. Simulation experiments show that BER performance of the NEGMIN equalizer with the other solution than the MMSE one has similar characteristics to the adaptive minimum bit error rate (AMBER) equalizer. The main advantage of the proposed equalizer is that it needs significantly fewer training symbols than the AMBER equalizer. Furthermore, the proposed equalizer is more robust to nonlinear distortions than the MMSE equalizer.

Mobile User Connectivity in Relay-Assisted Visible Light Communications.

PubMed

Pešek, Petr; Zvanovec, Stanislav; Chvojka, Petr; Bhatnagar, Manav R; Ghassemlooy, Zabih; Saxena, Prakriti

2018-04-07

In this paper, we investigate relay-assisted visible light communications (VLC) where a mobile user acts as a relay and forwards data from a transmitter to the end mobile user. We analyse the utilization of the amplify-and-forward (AF) and decode-and-forward (DF) relaying schemes. The focus of the paper is on analysis of the behavior of the mobile user acting as a relay while considering a realistic locations of the receivers and transmitters on a standard mobile phone, more specifically with two photodetectors on both sides of a mobile phone and a transmitting LED array located upright. We also investigate dependency of the bit error rate (BER) performance on the azimuth and elevation angles of the mobile relay device within a typical office environment. We provide a new analytical description of BER for AF and DF-based relays in VLC. In addition we compare AF and DF-based systems and show that DF offers a marginal improvement in the coverage area with a BER < 10 -3 and a data rate of 100 Mb/s. Numerical results also illustrate that relay-based systems offer a significant improvement in terms of the coverage compared to direct non-line of sight VLC links.

Nonlinear demodulation and channel coding in EBPSK scheme.

PubMed

Chen, Xianqing; Wu, Lenan

2012-01-01

The extended binary phase shift keying (EBPSK) is an efficient modulation technique, and a special impacting filter (SIF) is used in its demodulator to improve the bit error rate (BER) performance. However, the conventional threshold decision cannot achieve the optimum performance, and the SIF brings more difficulty in obtaining the posterior probability for LDPC decoding. In this paper, we concentrate not only on reducing the BER of demodulation, but also on providing accurate posterior probability estimates (PPEs). A new approach for the nonlinear demodulation based on the support vector machine (SVM) classifier is introduced. The SVM method which selects only a few sampling points from the filter output was used for getting PPEs. The simulation results show that the accurate posterior probability can be obtained with this method and the BER performance can be improved significantly by applying LDPC codes. Moreover, we analyzed the effect of getting the posterior probability with different methods and different sampling rates. We show that there are more advantages of the SVM method under bad condition and it is less sensitive to the sampling rate than other methods. Thus, SVM is an effective method for EBPSK demodulation and getting posterior probability for LDPC decoding.

Mobile User Connectivity in Relay-Assisted Visible Light Communications

PubMed Central

Pešek, Petr; Zvanovec, Stanislav; Chvojka, Petr; Bhatnagar, Manav R.; Ghassemlooy, Zabih; Saxena, Prakriti

2018-01-01

In this paper, we investigate relay-assisted visible light communications (VLC) where a mobile user acts as a relay and forwards data from a transmitter to the end mobile user. We analyse the utilization of the amplify-and-forward (AF) and decode-and-forward (DF) relaying schemes. The focus of the paper is on analysis of the behavior of the mobile user acting as a relay while considering a realistic locations of the receivers and transmitters on a standard mobile phone, more specifically with two photodetectors on both sides of a mobile phone and a transmitting LED array located upright. We also investigate dependency of the bit error rate (BER) performance on the azimuth and elevation angles of the mobile relay device within a typical office environment. We provide a new analytical description of BER for AF and DF-based relays in VLC. In addition we compare AF and DF-based systems and show that DF offers a marginal improvement in the coverage area with a BER < 10–3 and a data rate of 100 Mb/s. Numerical results also illustrate that relay-based systems offer a significant improvement in terms of the coverage compared to direct non-line of sight VLC links. PMID:29642432

Bit error rate performance of pi/4-DQPSK in a frequency-selective fast Rayleigh fading channel

NASA Technical Reports Server (NTRS)

Liu, Chia-Liang; Feher, Kamilo

1991-01-01

The bit error rate (BER) performance of pi/4-differential quadrature phase shift keying (DQPSK) modems in cellular mobile communication systems is derived and analyzed. The system is modeled as a frequency-selective fast Rayleigh fading channel corrupted by additive white Gaussian noise (AWGN) and co-channel interference (CCI). The probability density function of the phase difference between two consecutive symbols of M-ary differential phase shift keying (DPSK) signals is first derived. In M-ary DPSK systems, the information is completely contained in this phase difference. For pi/4-DQPSK, the BER is derived in a closed form and calculated directly. Numerical results show that for the 24 kBd (48 kb/s) pi/4-DQPSK operated at a carrier frequency of 850 MHz and C/I less than 20 dB, the BER will be dominated by CCI if the vehicular speed is below 100 mi/h. In this derivation, frequency-selective fading is modeled by two independent Rayleigh signal paths. Only one co-channel is assumed in this derivation. The results obtained are also shown to be valid for discriminator detection of M-ary DPSK signals.

Bio-electrochemical removal of nitrate from water and wastewater--a review.

PubMed

Ghafari, Shahin; Hasan, Masitah; Aroua, Mohamed Kheireddine

2008-07-01

Nitrates in different water and wastewater streams raised concerns due to severe impacts on human and animal health. Diverse methods are reported to remove nitrate from water streams which almost fail to entirely treat nitrate, except biological denitrification which is capable of reducing inorganic nitrate compounds to harmless nitrogen gas. Review of numerous studies in biological denitrification of nitrate containing water resources, aquaculture wastewaters and industrial wastewater confirmed the potential of this method and its flexibility towards the remediation of different concentrations of nitrate. The denitrifiers could be fed with organic and inorganic substrates which have different performances and subsequent advantages or disadvantages. Review of heterotrophic and autotrophic denitrifications with different food and energy sources concluded that autotrophic denitrifiers are more effective in denitrification. Autotrophs utilize carbon dioxide and hydrogen as the source of carbon substrate and electron donors, respectively. The application of this method in bio-electro reactors (BERs) has many advantages and is promising. However, this method is not so well established and documented. BERs provide proper environment for simultaneous hydrogen production on cathodes and appropriate consumption by immobilized autotrophs on these cathodes. This survey covers various designs and aspects of BERs and their performances.

Die Energiebranche am Beginn der digitalen Transformation: aus Versorgern werden Utilities 4.0

NASA Astrophysics Data System (ADS)

Doleski, Oliver D.

Die traditionelle Energieversorgung befindet sich in einer Zäsur. Das seit Jahrzehnten bewährte und stabile Geschäftsmodell einer zuverlässig planbaren, zentralen Energieerzeugung mit anschließender unidirektionaler Verteilung geriet spätestens seit der Energiewende des Jahres 2011 immer mehr unter Druck. Angesichts fortschreitender Digitalisierung und Dezentralisierung einerseits und gestiegener Erwartungshaltung der Kunden gegenüber "ihrem" Versorger andererseits sieht sich die Energiebranche heute einem epochalen Transformationsprozess gegenübergestellt. Bei diesem entwickeln sich aus den monopolistischen Versorgern des 19. und 20. Jahrhunderts (Utility 1.0), über die liberalisierten Energieversorgungsunternehmen (Utility 2.0) und Energiedienstleistungsunternehmen (Utility 3.0) heutiger Prägung, die digitalen Energiedienstleistungsunternehmen (Utility 4.0) von morgen. Oliver D. Doleski beschränkt sich in seinem Beitrag nicht auf die reine Beschreibung dieses Veränderungsprozesses an sich. Vielmehr werden darüber hinaus sowohl die mit dieser Entwicklung verbundenen übergeordneten Ziele als auch die wesentlichen Anforderungen an erfolgreiche Utilities 4.0 skizziert. Das Kapitel endet mit einem prägnanten Überblick profitabler Betätigungsfelder, die zur Sicherung der ökonomischen Zukunft von Energiekonzernen, Regionalversorgern und Stadtwerken beitragen können.

A Novel Multiple-Access Correlation-Delay-Shift-Keying

NASA Astrophysics Data System (ADS)

Duan, J. Y.; Jiang, G. P.; Yang, H.

In Correlation-Delay-Shift-Keying (CDSK), the reference signal and the information-bearing signal are added together during a certain time delay. Because the reference signal is not strictly orthogonal to the information-bearing signal, the cross-correlation between the adjacent chaotic signal (Intra-signal Interference, ISI) will be introduced into the demodulation at the receiver. Therefore, the Bit-Error Ratio (BER) of CDSK is higher than that of Differential-Chaos-Shift-Keying (DCSK). To avoid the ISI component and enhance the BER performance of CDSK in multiuser scenario, Multiple-Access CDSK with No Intra-signal Interference (MA-CDSK-NII) is proposed. By constructing the repeated chaotic generator and applying the Walsh code sequence to modulate the reference signal, in MA-CDSK-NII, the ISI component will be eliminated during the demodulation. Gaussian approximation method is adopted here to obtain the exact performance analysis of MA-CDSK-NII over additive white Gaussian noise (AWGN) channel and Rayleigh multipath fading channels. Results show that, due to no ISI component and lower transmitting power, the BER performance of MA-CDSK-NII can be better than that of multiple-access CDSK and Code-Shifted Differential-Chaos-Shift-Keying (CS-DCSK).

On the performance evaluation of LQAM-MPPM techniques over exponentiated Weibull fading free-space optical channels

NASA Astrophysics Data System (ADS)

Khallaf, Haitham S.; Elfiqi, Abdulaziz E.; Shalaby, Hossam M. H.; Sampei, Seiichi; Obayya, Salah S. A.

2018-06-01

We investigate the performance of hybrid L-ary quadrature-amplitude modulation-multi-pulse pulse-position modulation (LQAM-MPPM) techniques over exponentiated Weibull (EW) fading free-space optical (FSO) channel, considering both weather and pointing-error effects. Upper bound and approximate-tight upper bound expressions for the bit-error rate (BER) of LQAM-MPPM techniques over EW FSO channels are obtained, taking into account the effects of fog, beam divergence, and pointing-error. Setup block diagram for both the transmitter and receiver of the LQAM-MPPM/FSO system are introduced and illustrated. The BER expressions are evaluated numerically and the results reveal that LQAM-MPPM technique outperforms ordinary LQAM and MPPM schemes under different fading levels and weather conditions. Furthermore, the effect of modulation-index is investigated and it turned out that a modulation-index greater than 0.4 is required in order to optimize the system performance. Finally, the effect of pointing-error introduces a great power penalty on the LQAM-MPPM system performance. Specifically, at a BER of 10-9, pointing-error introduces power penalties of about 45 and 28 dB for receiver aperture sizes of DR = 50 and 200 mm, respectively.

Catadioptric lenses in Visible Light Communications

NASA Astrophysics Data System (ADS)

Garcia-Marquez, J.; Valencia, J. C.; Perez, H.; Topsu, S.

2015-04-01

Since few years ago, visible light communications (VLC) have experience an accelerated interest from a research point of view. The beginning of this decade has seen many improvements in VLC at an electronic level. High rates of transmission at low bit error ratios (BER) have been reported. A few numbers of start-ups have initiated activities to offer a variety of applications ranging from indoor geo-localization to internet, but in spite of these advancements, some other problems arise. Long-range transmissions mean a high BER which reduce the number of applications. In this sense, new redesigned optical collectors or in some cases, optical reflectors must be considered to ensure a low BER at higher distance transmissions. Here we also expose a preliminary design of a catadioptric and monolithical lens for a LI-FI receiver with two rotationally symmetrical main piecewise surfaces za and zb. These surfaces are represented in a system of cylindrical coordinates with an anterior surface za with a central and refractive sector surrounded by a peripheral reflective sector and a back piecewise surface zb with a central refractive sector and a reflective sector, both characterized as ideal for capturing light within large acceptance angles.

A digital clock recovery algorithm based on chromatic dispersion and polarization mode dispersion feedback dual phase detection for coherent optical transmission systems

NASA Astrophysics Data System (ADS)

Liu, Bo; Xin, Xiangjun; Zhang, Lijia; Wang, Fu; Zhang, Qi

2018-02-01

A new feedback symbol timing recovery technique using timing estimation joint equalization is proposed for digital receivers with two samples/symbol or higher sampling rate. Different from traditional methods, the clock recovery algorithm in this paper adopts another algorithm distinguishing the phases of adjacent symbols, so as to accurately estimate the timing offset based on the adjacent signals with the same phase. The addition of the module for eliminating phase modulation interference before timing estimation further reduce the variance, thus resulting in a smoothed timing estimate. The Mean Square Error (MSE) and Bit Error Rate (BER) of the resulting timing estimate are simulated to allow a satisfactory estimation performance. The obtained clock tone performance is satisfactory for MQAM modulation formats and the Roll-off Factor (ROF) close to 0. In the back-to-back system, when ROF= 0, the maximum of MSE obtained with the proposed approach reaches 0 . 0125. After 100-km fiber transmission, BER decreases to 10-3 with ROF= 0 and OSNR = 11 dB. With the increase in ROF, the performances of MSE and BER become better.

Induction of NEIL1 and NEIL2 DNA glycosylases in aniline-induced splenic toxicity

PubMed Central

Ma, Huaxian; Wang, Jianling; Abdel-Rahman, Sherif Z.; Hazra, Tapas K.; Boor, Paul J.; Khan, M. Firoze

2011-01-01

The mechanisms by which aniline exposure elicits splenotoxic response, especially the tumorigenic response, are not well-understood. Earlier, we have shown that aniline-induced oxidative stress is associated with increased oxidative DNA damage in rat spleen. The base excision repair (BER) pathway is the major mechanism for the repair of oxidative DNA base lesions, and we have shown an up-regulation of 8-oxoguanine glycosylase 1 (OGG1), a specific DNA glycosylase involved in the removal of 8-hydroxy-2′-deoxyguanosine (8-OHdG) adducts, following aniline exposure. Nei-like DNA glycosylases (NEIL1/2) belong to a family of BER proteins that are distinct from other DNA glycosylases, including OGG1. However, contribution of NEIL1/2 in the repair of aniline-induced oxidative DNA damage in the spleen is not known. This study was, therefore, focused on evaluating if NEILs also contribute to the repair of oxidative DNA lesions in the spleen following aniline exposure. To achieve that, male SD rats were subchronically exposed to aniline (0.5 mmol/kg/day via drinking water for 30 days), while controls received drinking water only. The BER activity of NEIL1/2 was assayed using a bubble structure substrate containing 5-OHU (preferred substrates for NEIL1 and NEIL2) and by quantitating the cleavage products. Aniline treatment led to a 1.25-fold increase in the NEIL1/2-associated BER activity in the nuclear extracts of spleen compared to the controls. Real-time PCR analysis for NEIL1 and NEIL2 mRNA expression in the spleen revealed 2.7- and 3.9-fold increases, respectively, in aniline-treated rats compared to controls. Likewise, Western blot analysis showed that protein expression of NEIL1 and NEIL2 in the nuclear extract of spleens from aniline-treated rats was 2.0- and 3.8-fold higher than controls, respectively. Aniline treatment also led to stronger immunoreactivity for NEIL1 and NEIL2 in the spleens, confined to the red pulp areas. These studies, thus, show that aniline-induced oxidative stress is associated with an induction of NEIL1/2. The increased NIELs-mediated BER activity is another indication of aniline-induced oxidative damage in the spleen and could constitute another important mechanism of removal of oxidative DNA lesions, especially in transcribed DNA following aniline insult. PMID:21145906

The ASP Sensor Network: Infrastructure for the Next Generation of NASA Airborne Science

NASA Astrophysics Data System (ADS)

Myers, J. S.; Sorenson, C. E.; Van Gilst, D. P.; Duley, A.

2012-12-01

A state-of-the-art real-time data communications network is being implemented across the NASA Airborne Science Program core platforms. Utilizing onboard Ethernet networks and satellite communications systems, it is intended to maximize the science return from both single-platform missions and complex multi-aircraft Earth science campaigns. It also provides an open platform for data visualization and synthesis software tools, for use by the science instrument community. This paper will describe the prototype implementations currently deployed on the NASA DC-8 and Global Hawk aircraft, and the ongoing effort to expand the capability to other science platforms. Emphasis will be on the basic network architecture, the enabling hardware, and new standardized instrument interfaces. The new Mission Tools Suite, which provides an web-based user interface, will be also described; together with several example use-cases of this evolving technology.

Networking Course Syllabus in Accredited Library and Information Science Programs: A Comparative Analysis Study

ERIC Educational Resources Information Center

Abouserie, Hossam Eldin Mohamed Refaat

2009-01-01

The study investigated networking courses offered in accredited Library and Information Science schools in the United States in 2009. The study analyzed and compared network syllabi according to Course Syllabus Evaluation Rubric to obtain in-depth understanding of basic features and characteristics of networking courses taught. The study embraced…

Social Networking among Library and Information Science Undergraduate Students

ERIC Educational Resources Information Center

Alakpodia, Onome Norah

2015-01-01

The purpose of this study was to examine social networking use among Library and Information Science students of the Delta State University, Abraka. In this study, students completed a questionnaire which assessed their familiarity with social networking sites, the purpose for which they use social networking site and their most preferred sites to…

Development and Evaluation of a City-Wide Wireless Weather Sensor Network

ERIC Educational Resources Information Center

Chang, Ben; Wang, Hsue-Yie; Peng, Tian-Yin; Hsu, Ying-Shao

2010-01-01

This project analyzed the effectiveness of a city-wide wireless weather sensor network, the Taipei Weather Science Learning Network (TWIN), in facilitating elementary and junior high students' study of weather science. The network, composed of sixty school-based weather sensor nodes and a centralized weather data archive server, provides students…

On Several Fundamental Problems of Optimization, Estimation, and Scheduling in Wireless Communications

NASA Astrophysics Data System (ADS)

Gao, Qian

For both the conventional radio frequency and the comparably recent optical wireless communication systems, extensive effort from the academia had been made in improving the network spectrum efficiency and/or reducing the error rate. To achieve these goals, many fundamental challenges such as power efficient constellation design, nonlinear distortion mitigation, channel training design, network scheduling and etc. need to be properly addressed. In this dissertation, novel schemes are proposed accordingly to deal with specific problems falling in category of these challenges. Rigorous proofs and analyses are provided for each of our work to make a fair comparison with the corresponding peer works to clearly demonstrate the advantages. The first part of this dissertation considers a multi-carrier optical wireless system employing intensity modulation (IM) and direct detection (DD). A block-wise constellation design is presented, which treats the DC-bias that conventionally used solely for biasing purpose as an information basis. Our scheme, we term it MSM-JDCM, takes advantage of the compactness of sphere packing in a higher dimensional space, and in turn power efficient constellations are obtained by solving an advanced convex optimization problem. Besides the significant power gains, the MSM-JDCM has many other merits such as being capable of mitigating nonlinear distortion by including a peak-to-power ratio (PAPR) constraint, minimizing inter-symbol-interference (ISI) caused by frequency-selective fading with a novel precoder designed and embedded, and further reducing the bit-error-rate (BER) by combining with an optimized labeling scheme. The second part addresses several optimization problems in a multi-color visible light communication system, including power efficient constellation design, joint pre-equalizer and constellation design, and modeling of different structured channels with cross-talks. Our novel constellation design scheme, termed CSK-Advanced, is compared with the conventional decoupled system with the same spectrum efficiency to demonstrate the power efficiency. Crucial lighting requirements are included as optimization constraints. To control non-linear distortion, the optical peak-to-average-power ratio (PAPR) of LEDs can be individually constrained. With a SVD-based pre-equalizer designed and employed, our scheme can achieve lower BER than counterparts applying zero-forcing (ZF) or linear minimum-mean-squared-error (LMMSE) based post-equalizers. Besides, a binary switching algorithm (BSA) is applied to improve BER performance. The third part looks into a problem of two-phase channel estimation in a relayed wireless network. The channel estimates in every phase are obtained by the linear minimum mean squared error (LMMSE) method. Inaccurate estimate of the relay to destination (RtD) channel in phase 1 could affect estimate of the source to relay (StR) channel in phase 2, which is made erroneous. We first derive a close-form expression for the averaged Bayesian mean-square estimation error (ABMSE) for both phase estimates in terms of the length of source and relay training slots, based on which an iterative searching algorithm is then proposed that optimally allocates training slots to the two phases such that estimation errors are balanced. Analysis shows how the ABMSE of the StD channel estimation varies with the lengths of relay training and source training slots, the relay amplification gain, and the channel prior information respectively. The last part deals with a transmission scheduling problem in a uplink multiple-input-multiple-output (MIMO) wireless network. Code division multiple access (CDMA) is assumed as a multiple access scheme and pseudo-random codes are employed for different users. We consider a heavy traffic scenario, in which each user always has packets to transmit in the scheduled time slots. If the relay is scheduled for transmission together with users, then it operates in a full-duplex mode, where the packets previously collected from users are transmitted to the destination while new packets are being collected from users. A novel expression of throughput is first derived and then used to develop a scheduling algorithm to maximize the throughput. Our full-duplex scheduling is compared with a half-duplex scheduling, random access, and time division multiple access (TDMA), and simulation results illustrate its superiority. Throughput gains due to employment of both MIMO and CDMA are observed.

Reflections from organization science on the development of primary health care research networks.

PubMed

Fenton, E; Harvey, J; Griffiths, F; Wild, A; Sturt, J

2001-10-01

In the UK, policy changes in primary health care research and development have led to the establishment of primary care research networks. These organizations aim to increase research culture, capacity and evidence base in primary care. As publicly funded bodies, these networks need to be accountable. Organizational science has studied network organizations including why and how they develop and how they function most effectively. This paper draws on organizational science to reflect on why primary care research networks appear to be appropriate for primary care research and how their structures and processes can best enable the achievement of their aims.

Who Do You Know? Developing and Analyzing Entrepreneur Networks: Data Collection in the Tech Entrepreneurial Environment of Six African Cities

DTIC Science & Technology

2015-01-01

Prescribed by ANSI Std. Z39.18 4 1 | P a g e Network Science Center, West Point www.netscience.usma.edu 845.938.0804 January 2015...in detail in our 2 | P a g e Network Science Center, West Point www.netscience.usma.edu 845.938.0804 previous methodology paper. Based on...one of the leading universities in Sub-Saharan Africa. The student body is energetic and tech savvy. 3 | P a g e Network Science Center, West Point

Modern network science of neurological disorders.

PubMed

Stam, Cornelis J

2014-10-01

Modern network science has revealed fundamental aspects of normal brain-network organization, such as small-world and scale-free patterns, hierarchical modularity, hubs and rich clubs. The next challenge is to use this knowledge to gain a better understanding of brain disease. Recent developments in the application of network science to conditions such as Alzheimer's disease, multiple sclerosis, traumatic brain injury and epilepsy have challenged the classical concept of neurological disorders being either 'local' or 'global', and have pointed to the overload and failure of hubs as a possible final common pathway in neurological disorders.

Wildland fire science and management in the U.S.: Spanning the boundaries through the regional knowledge exchange network (Abstract)

Treesearch

Susie Kocher; Eric Toman; Sarah Trainor; Vita Wright

2012-01-01

In 2009, the federal Joint Fire Science Program (JFSP) initiated a national network of regional fire science consortia to accelerate awareness, understanding and use of wildland fire science. This presentation synthesizes findings from initial needs assessments conducted by consortia in eight regions of the United States. The assessments evaluated how fire science is...

A social network approach to understanding science communication among fire professionals

Treesearch

Vita Wright

2012-01-01

Studies of science communication and use in the fire management community suggest manager's access research via informal information networks and that these networks vary by both agency and position. We used a phone survey followed by traditional statistical analyses to understand the informal social networks of fire professionals in two western regions of the...

A social network approach to understanding science communication among fire professionals (Abstract)

Treesearch

Vita Wright; Andrea Thode; Anne Mottek-Lucas; Jacklynn Fallon; Megan Matonis

2012-01-01

Studies of science communication and use in the fire management community suggest manager's access research via informal information networks and that these networks vary by both agency and position. We used a phone survey followed by traditional statistical analyses to understand the informal social networks of fire professionals in two western regions of the...

The Community Science Workshop Network Story: Case Studies of the CSW Sites

ERIC Educational Resources Information Center

St. John, Mark

2014-01-01

The Community Science Workshops (CSWs)--with funding from the S.D. Bechtel, Jr. Foundation, and the Gordon and Betty Moore Foundation--created a network among the CSW sites in California. The goals of the CSW Network project have been to improve programs, build capacity throughout the Network, and establish new sites. Inverness Research has been…

Know the Network, Knit the Network: Applying SNA to N2C2 Maturity Model Experiments

DTIC Science & Technology

2010-06-01

Networks (COINS) 2009. Procedia - Social and Behavioral Sciences (2009). Snijders, Tom A.B., Christian E. G. Steglich and Michael Schweinberger...8217 patterning that create social structures. As an interdisciplinary behavioural science specialty, SNA defends that social actors are interdependent...production of social science data involve a process of interpretation. To carry out such interpretation robustly it is understood that it is imperative to

Analysis and logical modeling of biological signaling transduction networks

NASA Astrophysics Data System (ADS)

Sun, Zhongyao

The study of network theory and its application span across a multitude of seemingly disparate fields of science and technology: computer science, biology, social science, linguistics, etc. It is the intrinsic similarities embedded in the entities and the way they interact with one another in these systems that link them together. In this dissertation, I present from both the aspect of theoretical analysis and the aspect of application three projects, which primarily focus on signal transduction networks in biology. In these projects, I assembled a network model through extensively perusing literature, performed model-based simulations and validation, analyzed network topology, and proposed a novel network measure. The application of network modeling to the system of stomatal opening in plants revealed a fundamental question about the process that has been left unanswered in decades. The novel measure of the redundancy of signal transduction networks with Boolean dynamics by calculating its maximum node-independent elementary signaling mode set accurately predicts the effect of single node knockout in such signaling processes. The three projects as an organic whole advance the understanding of a real system as well as the behavior of such network models, giving me an opportunity to take a glimpse at the dazzling facets of the immense world of network science.

The BIOSCI electronic newsgroup network for the biological sciences. Final report, October 1, 1992--June 30, 1996

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kristofferson, D.; Mack, D.

1996-10-01

This is the final report for a DOE funded project on BIOSCI Electronic Newsgroup Network for the biological sciences. A usable network for scientific discussion, major announcements, problem solving, etc. has been created.

Experimental demonstration of large capacity WSDM optical access network with multicore fibers and advanced modulation formats.

PubMed

Li, Borui; Feng, Zhenhua; Tang, Ming; Xu, Zhilin; Fu, Songnian; Wu, Qiong; Deng, Lei; Tong, Weijun; Liu, Shuang; Shum, Perry Ping

2015-05-04

Towards the next generation optical access network supporting large capacity data transmission to enormous number of users covering a wider area, we proposed a hybrid wavelength-space division multiplexing (WSDM) optical access network architecture utilizing multicore fibers with advanced modulation formats. As a proof of concept, we experimentally demonstrated a WSDM optical access network with duplex transmission using our developed and fabricated multicore (7-core) fibers with 58.7km distance. As a cost-effective modulation scheme for access network, the optical OFDM-QPSK signal has been intensity modulated on the downstream transmission in the optical line terminal (OLT) and it was directly detected in the optical network unit (ONU) after MCF transmission. 10 wavelengths with 25GHz channel spacing from an optical comb generator are employed and each wavelength is loaded with 5Gb/s OFDM-QPSK signal. After amplification, power splitting, and fan-in multiplexer, 10-wavelength downstream signal was injected into six outer layer cores simultaneously and the aggregation downstream capacity reaches 300 Gb/s. -16 dBm sensitivity has been achieved for 3.8 × 10^-3 bit error ratio (BER) with 7% Forward Error Correction (FEC) limit for all wavelengths in every core. Upstream signal from ONU side has also been generated and the bidirectional transmission in the same core causes negligible performance degradation to the downstream signal. As a universal platform for wired/wireless data access, our proposed architecture provides additional dimension for high speed mobile signal transmission and we hence demonstrated an upstream delivery of 20Gb/s per wavelength with QPSK modulation formats using the inner core of MCF emulating a mobile backhaul service. The IQ modulated data was coherently detected in the OLT side. -19 dBm sensitivity has been achieved under the FEC limit and more than 18 dB power budget is guaranteed.

Neural Networks In Mining Sciences - General Overview And Some Representative Examples

NASA Astrophysics Data System (ADS)

Tadeusiewicz, Ryszard

2015-12-01

The many difficult problems that must now be addressed in mining sciences make us search for ever newer and more efficient computer tools that can be used to solve those problems. Among the numerous tools of this type, there are neural networks presented in this article - which, although not yet widely used in mining sciences, are certainly worth consideration. Neural networks are a technique which belongs to so called artificial intelligence, and originates from the attempts to model the structure and functioning of biological nervous systems. Initially constructed and tested exclusively out of scientific curiosity, as computer models of parts of the human brain, neural networks have become a surprisingly effective calculation tool in many areas: in technology, medicine, economics, and even social sciences. Unfortunately, they are relatively rarely used in mining sciences and mining technology. The article is intended to convince the readers that neural networks can be very useful also in mining sciences. It contains information how modern neural networks are built, how they operate and how one can use them. The preliminary discussion presented in this paper can help the reader gain an opinion whether this is a tool with handy properties, useful for him, and what it might come in useful for. Of course, the brief introduction to neural networks contained in this paper will not be enough for the readers who get convinced by the arguments contained here, and want to use neural networks. They will still need a considerable portion of detailed knowledge so that they can begin to independently create and build such networks, and use them in practice. However, an interested reader who decides to try out the capabilities of neural networks will also find here links to references that will allow him to start exploration of neural networks fast, and then work with this handy tool efficiently. This will be easy, because there are currently quite a few ready-made computer programs, easily available, which allow their user to quickly and effortlessly create artificial neural networks, run them, train and use in practice. The key issue is the question how to use these networks in mining sciences. The fact that this is possible and desirable is shown by convincing examples included in the second part of this study. From the very rich literature on the various applications of neural networks, we have selected several works that show how and what neural networks are used in the mining industry, and what has been achieved thanks to their use. The review of applications will continue in the next article, filed already for publication in the journal "Archives of Mining Sciences". Only studying these two articles will provide sufficient knowledge for initial guidance in the area of issues under consideration here.

Ultra-high capacity WDM-SDM optical access network with self-homodyne detection downstream and 32QAM-FBMC upstream.

PubMed

Feng, Zhenhua; Xu, Liang; Wu, Qiong; Tang, Ming; Fu, Songnian; Tong, Weijun; Shum, Perry Ping; Liu, Deming

2017-03-20

Towards 100G beyond large-capacity optical access networks, wavelength division multiplexing (WDM) techniques incorporating with space division multiplexing (SDM) and affordable spectrally efficient advanced modulation formats are indispensable. In this paper, we proposed and experimentally demonstrated a cost-efficient multicore fiber (MCF) based hybrid WDM-SDM optical access network with self-homodyne coherent detection (SHCD) based downstream (DS) and direct detection optical filter bank multi carrier (DDO-FBMC) based upstream (US). In the DS experiments, the inner core of the 7-core fiber is used as a dedicated channel to deliver the local oscillator (LO) lights while the other 6 outer cores are used to transmit 4 channels of wavelength multiplexed 200-Gb/s PDM-16QAM-OFDM signals. For US transmission, 4 wavelengths with channel spacing of 100 GHz are intensity modulated with 30 Gb/s 32-QAM-FBMC and directly detected by a ~7 GHz bandwidth receiver after transmission along one of the outer core. The results show that a 4 × 6 × 200-Gb/s DS transmission can be realized over 37 km 7-core fiber without carrier frequency offset (CFO) and phase noise (PN) compensation even using 10 MHz linewidth DFB lasers. The SHCD based on MCF provides a compromise and cost efficient scheme between conventional intradyne coherent detection and intensity modulation and direct detection (IM/DD) schemes. Both US and DS have acceptable BER performance and high spectral efficiency.

Enhancing Use of Learning Sciences Research in Planning for and Supporting Educational Change: Leveraging and Building Social Networks

ERIC Educational Resources Information Center

Penuel, William R.; Bell, Philip; Bevan, Bronwyn; Buffington, Pam; Falk, Joni

2016-01-01

This paper explores practical ways to engage two areas of educational scholarship--research on science learning and research on social networks--to inform efforts to plan and support implementation of new standards. The standards, the "Next Generation Science Standards" (NGSS; NGSS Lead States in Next generation science standards: For…

On Real-Time Systems Using Local Area Networks.

DTIC Science & Technology

1987-07-01

87-35 July, 1987 CS-TR-1892 On Real - Time Systems Using Local Area Networks*I VShem-Tov Levi Department of Computer Science Satish K. Tripathit...1892 On Real - Time Systems Using Local Area Networks* Shem-Tov Levi Department of Computer Science Satish K. Tripathit Department of Computer Science...constraints and the clock systems that feed the time to real - time systems . A model for real-time system based on LAN communication is presented in

High Performance Computing and Network Program. Hearing before the Subcommittee on Science of the Committee on Science, Space, and Technology, House of Representatives, One Hundred Third Congress, First Session.

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. House Committee on Science, Space and Technology.

The purpose of the hearing transcribed in this document was to obtain the views of representatives of network user and provider communities regarding the path the National Science Foundation (NSF) is taking for recompetition of the NSFNET computer network. In particular the committee was interested in the consistency of the evolution of NSFNET…

Network Science Center Research Teams Visit to Addis Ababa, Ethiopia

DTIC Science & Technology

2012-08-01

Network Science Center, West Point www.netscience.usma.edu 845.938.0804 Corporation as a gift from the Government of China, and consists of a 2,500... German Government in achieving its objectives in the field of international cooperation for sustainable development. Construction of Road...authors and do not reflect the official policy or position of the Department of Defense or the U.S. Government . 14. ABSTRACT A Network Science Center

Using Network Science Measures to Predict the Lexical Decision Performance of Adults Who Stutter.

PubMed

Castro, Nichol; Pelczarski, Kristin M; Vitevitch, Michael S

2017-07-12

Methods from network science have examined various aspects of language processing. Clinical populations may also benefit from these novel analyses. Phonological and lexical factors have been examined in adults who stutter (AWS) as potential contributing factors to stuttering, although differences reported are often subtle. We reexamined the performance of AWS and adults who do not stutter (AWNS) from a previously conducted lexical decision task in an attempt to determine if network science measures would provide additional insight into the phonological network of AWS beyond traditional psycholinguistic measures. Multiple regression was used to examine the influence of several traditional psycholinguistic measures as well as several new measures from network science on response times. AWS responded to low-frequency words more slowly than AWNS; responses for both groups were equivalent for high-frequency words. AWS responded to shorter words more slowly than AWNS, producing a reverse word-length effect. For the network measures, degree/neighborhood density and closeness centrality, but not whether a word was inside or outside the giant component, influenced response times similarly between groups. Network analyses suggest that multiple levels of the phonological network might influence phonological processing, not just the micro-level traditionally considered by mainstream psycholinguistics.

Performance and capacity analysis of Poisson photon-counting based Iter-PIC OCDMA systems.

PubMed

Li, Lingbin; Zhou, Xiaolin; Zhang, Rong; Zhang, Dingchen; Hanzo, Lajos

2013-11-04

In this paper, an iterative parallel interference cancellation (Iter-PIC) technique is developed for optical code-division multiple-access (OCDMA) systems relying on shot-noise limited Poisson photon-counting reception. The novel semi-analytical tool of extrinsic information transfer (EXIT) charts is used for analysing both the bit error rate (BER) performance as well as the channel capacity of these systems and the results are verified by Monte Carlo simulations. The proposed Iter-PIC OCDMA system is capable of achieving two orders of magnitude BER improvements and a 0.1 nats of capacity improvement over the conventional chip-level OCDMA systems at a coding rate of 1/10.

LDPC-coded orbital angular momentum (OAM) modulation for free-space optical communication.

PubMed

Djordjevic, Ivan B; Arabaci, Murat

2010-11-22

An orbital angular momentum (OAM) based LDPC-coded modulation scheme suitable for use in FSO communication is proposed. We demonstrate that the proposed scheme can operate under strong atmospheric turbulence regime and enable 100 Gb/s optical transmission while employing 10 Gb/s components. Both binary and nonbinary LDPC-coded OAM modulations are studied. In addition to providing better BER performance, the nonbinary LDPC-coded modulation reduces overall decoder complexity and latency. The nonbinary LDPC-coded OAM modulation provides a net coding gain of 9.3 dB at the BER of 10(-8). The maximum-ratio combining scheme outperforms the corresponding equal-gain combining scheme by almost 2.5 dB.

Performance of the image statistics decoder in conjunction with the Goldstone-VLA array

NASA Technical Reports Server (NTRS)

Wang, H. C.; Pitt, G. H., III

1989-01-01

During Voyager's Neptune encounter, the National Radio Astronomy Observatory's Very Large Array (VLA) will be arrayed with Goldstone antennas to receive the transmitted telemetry data from the spacecraft. The telemetry signal from the VLA will drop out periodically, resulting in a periodic drop in the received signal-to-noise ratio (SNR). The Image Statistics Decoder (ISD), which assumes a correlation between pixels, can improve the bit error rate (BER) for images during these dropout periods. Simulation results have shown that the ISD, in conjunction with the Goldstone-VLA array can provide a 3-dB gain for uncompressed images at a BER of 5.0 x 10(exp -3).

Experimental study of non-binary LDPC coding for long-haul coherent optical QPSK transmissions.

PubMed

Zhang, Shaoliang; Arabaci, Murat; Yaman, Fatih; Djordjevic, Ivan B; Xu, Lei; Wang, Ting; Inada, Yoshihisa; Ogata, Takaaki; Aoki, Yasuhiro

2011-09-26

The performance of rate-0.8 4-ary LDPC code has been studied in a 50 GHz-spaced 40 Gb/s DWDM system with PDM-QPSK modulation. The net effective coding gain of 10 dB is obtained at BER of 10(-6). With the aid of time-interleaving polarization multiplexing and MAP detection, 10,560 km transmission over legacy dispersion managed fiber is achieved without any countable errors. The proposed nonbinary quasi-cyclic LDPC code achieves an uncoded BER threshold at 4×10(-2). Potential issues like phase ambiguity and coding length are also discussed when implementing LDPC in current coherent optical systems. © 2011 Optical Society of America

Analytical performance study of solar blind non-line-of-sight ultraviolet short-range communication links.

PubMed

Xu, Zhengyuan; Ding, Haipeng; Sadler, Brian M; Chen, Gang

2008-08-15

Motivated by recent advances in solid-state incoherent ultraviolet sources and solar blind detectors, we study communication link performance over a range of less than 1 km with a bit error rate (BER) below 10(-3) in solar blind non-line-of-sight situation. The widely adopted yet complex single scattering channel model is significantly simplified by means of a closed-form expression for tractable analysis. Path loss is given as a function of transceiver geometry as well as atmospheric scattering and attenuation and is compared with experimental data for model validation. The BER performance of a shot-noise-limited receiver under this channel model is demonstrated.

Performance Analysis of HF Band FB-MC-SS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hussein Moradi; Stephen Andrew Laraway; Behrouz Farhang-Boroujeny

Abstract—In a recent paper [1] the filter bank multicarrier spread spectrum (FB-MC-SS) waveform was proposed for wideband spread spectrum HF communications. A significant benefit of this waveform is robustness against narrow and partial band interference. Simulation results in [1] demonstrated good performance in a wideband HF channel over a wide range of conditions. In this paper we present a theoretical analysis of the bit error probably for this system. Our analysis tailors the results from [2] where BER performance was analyzed for maximum ration combining systems that accounted for correlation between subcarriers and channel estimation error. Equations are give formore » BER that closely match the simulated performance in most situations.« less

On the contribution of Heinrich Bruns to theoretical geometrical optics. With consideration of his correspondence with scientists of the Zeiss Company in Jena 1888-1893. (German Title: Über den Beitrag von Heinrich Bruns zur theoretischen geometrischen Optik Unter Berücksichtigung seines Briefwechsels mit Wissenschaftlern der Zeiss-Werke in Jena 1888-1893)

NASA Astrophysics Data System (ADS)

Ilgauds, Hans-Joachim; Münzel, Gisela

This paper describes the works of Heinrich Bruns, director of the Leipzig University Observatory, on theoretical geometrical optics, which followed an outstanding tradition in Leipzig. Bruns and his pupils did not stop at theoretical considerations, but applied their findings to practical questions. Bruns' correspondence with opticians of the Zeiss Company in Jena, so far known only fragmentarily, gives impressive evidence of their friendly relationship characterized by mutual regard and stimulation.

Performance analysis of 1-km free-space optical communication system over real atmospheric turbulence channels

NASA Astrophysics Data System (ADS)

Liu, Dachang; Wang, Zixiong; Liu, Jianguo; Tan, Jun; Yu, Lijuan; Mei, Haiping; Zhou, Yusong; Zhu, Ninghua

2017-10-01

The performance of a free-space optical communication system is highly affected by the atmospheric turbulence in terms of scintillation. An optical communication system based on intensity-modulation direct-detection was built with 1-km transmission distance to evaluate the bit error rate (BER) performance over real atmospheric turbulence. 2.5-, 5-, and 10-Gbps data rate transmissions were carried out, where error-free transmission could be achieved during over 37% of the 2.5-Gbps transmissions and over 43% of the 5-Gbps transmissions. In the rest of the transmissions, BER deteriorated as the refractive-index structure constant increased, while the two measured items have almost the same trend.

Isotrope und homogene Materie - Kosmen; On Dynamics and Thermodynamics of Isotropic Matter-Universes

NASA Astrophysics Data System (ADS)

Treder, H.-J.

Die Dynamik und Thermodynamik großer kosmischer Systeme ist fast unabhängig von den besonderen Theorien über die Gravitation. Nur die Feinstruktur der Kosmologie und Kosmonogie reflektiert die speziellen Hypothesen. Diese Neutralität gegenüber den konkreten Gravodynamiken ist die Konsequenz der fundamentalen Eigenschaften der Gravitation: Der Prinzipien der Äquivalenz von Trägheit und Schwere. The dynamics and thermodynamics of great cosmical systems are nearly independent of the theory of gravitation and only the fine-structure of cosmogony and cosmology reflects the special hypotheses.The neutrality against the concret gravodynamics is a consequence of the fundamental properties of gravitation: the principlies of equivalence of gravity and inertia.

Base Excision Repair of Oxidative DNA Damage

PubMed Central

David, Sheila S.; O’Shea, Valerie L.; Kundu, Sucharita

2010-01-01

Base excision repair plays an important role in preventing mutations associated with the common product of oxidative damage, 8-oxoguanine. Recent structural studies have shown that 8-oxoguanine glycosylases use an intricate series of steps to efficiently search and locate 8-oxoguanine lesions within the multitude of undamaged bases. The importance of prevention of mutations associated with 8-oxoguanine has also been illustrated by direct connections between defects in the BER glycosylase MUTYH and colorectal cancer. In addition, the properties of other guanine oxidation products and the BER glycosylases that remove them are being uncovered. This work is providing surprising and intriguing new insights into the process of base excision repair. PMID:17581577

Supercomputer networking for space science applications

NASA Technical Reports Server (NTRS)

Edelson, B. I.

1992-01-01

The initial design of a supercomputer network topology including the design of the communications nodes along with the communications interface hardware and software is covered. Several space science applications that are proposed experiments by GSFC and JPL for a supercomputer network using the NASA ACTS satellite are also reported.

Cooperative wireless network control based health and activity monitoring system.

PubMed

Prakash, R; Ganesh, A Balaji; Girish, Siva V

2016-10-01

A real-time cooperative communication based wireless network is presented for monitoring health and activity of an end-user in their environment. The cooperative communication offers better energy consumption and also an opportunity to aware the current location of a user non-intrusively. The link between mobile sensor node and relay node is dynamically established by using Received Signal Strength Indicator (RSSI) and Link Quality Indicator (LQI) based on adaptive relay selection scheme. The study proposes a Linear Acceleration based Transmission Power Decision Control (LA-TPDC) algorithm to further enhance the energy efficiency of cooperative communication. Further, the occurrences of false alarms are carefully prevented by introducing three stages of sequential warning system. The real-time experiments are carried-out by using the nodes, namely mobile sensor node, relay nodes and a destination node which are indigenously developed by using a CC430 microcontroller integrated with an in-built transceiver at 868 MHz. The wireless node performance characteristics, such as energy consumption, Signal-Noise ratio (SNR), Bit Error Rate (BER), Packet Delivery Ratio (PDR) and transmission offset are evaluated for all the participated nodes. The experimental results observed that the proposed linear acceleration based transmission power decision control algorithm almost doubles the battery life time than energy efficient conventional cooperative communication.

High efficiency diffraction grating technologies LPDL 900 and LPDL 1100 in telecommunications applications

NASA Astrophysics Data System (ADS)

Milner, Darrin; Didona, Kevin; Bannon, David

2005-04-01

With the introduction of wavelength division multiplexing and dense wavelength division multiplexing, equipment manufactures have sought to reduce design tradeoffs and costs while maintaining or increasing their product performance. With the need to reduce if not eliminate optical losses and create the all light path from source to destination, equipment manufactures are addressing the concerns of component manufactures to provide increased performance to support configurable designs for 100, 50, and eventually 12.5GHz. One of the most reliable, robust, and high performance devices is the low polarization dependent loss (LPDL) diffraction grating used to disperse wavelengths for channel blocking, add/drop functionality and real time light path reconfigurations. The networks today have a variety of factors which contribute to the optical loss budget and impact system design cost, facility requirements, maintenance or replacement costs. These factors include first and second order polarization mode dispersion (PMD), polarization dependent loss (PDL), wavelength dependent losses, and chromatic dispersion (CD). Network designers and equipment manufactures have to consider each component capability and its impact to the systems bit error rate (BER). In order to gain an understanding of the advantages of components with low polarization dependency, we will summarize the effects that interplay with these types of components.

Performance of an optical equalizer in a 10 G wavelength converting optical access network.

PubMed

Mendinueta, José Manuel D; Cao, Bowen; Thomsen, Benn C; Mitchell, John E

2011-12-12

A centralized optical processing unit (COPU) that functions both as a wavelength converter (WC) and optical burst equaliser in a 10 Gb/s wavelength-converting optical access network is proposed and experimentally characterized. This COPU is designed to consolidate drifting wavelengths generated with an uncooled laser in the upstream direction into a stable wavelength channel for WDM backhaul transmission and to equalize the optical loud/soft burst power in order to relax the burst-mode receiver dynamic range requirement. The COPU consists of an optical power equaliser composed of two cascaded SOAs followed by a WC. Using an optical packet generator and a DC-coupled PIN-based digital burst-mode receiver, the COPU is characterized in terms of payload-BER for back-to-back and backhaul transmission distances of 22, 40, and 62 km. We show that there is a compromise between the receiver sensitivity and overload points that can be optimized tuning the WC operating point for a particular backhaul fiber transmission distance. Using the optimized settings, sensitivities of -30.94, -30.17, and -27.26 dBm with overloads of -9.3, -5, and >-5 dBm were demonstrated for backhaul transmission distances of 22, 40 and 62 km, respectively. © 2011 Optical Society of America

Analysis of Optical CDMA Signal Transmission: Capacity Limits and Simulation Results

NASA Astrophysics Data System (ADS)

Garba, Aminata A.; Yim, Raymond M. H.; Bajcsy, Jan; Chen, Lawrence R.

2005-12-01

We present performance limits of the optical code-division multiple-access (OCDMA) networks. In particular, we evaluate the information-theoretical capacity of the OCDMA transmission when single-user detection (SUD) is used by the receiver. First, we model the OCDMA transmission as a discrete memoryless channel, evaluate its capacity when binary modulation is used in the interference-limited (noiseless) case, and extend this analysis to the case when additive white Gaussian noise (AWGN) is corrupting the received signals. Next, we analyze the benefits of using nonbinary signaling for increasing the throughput of optical CDMA transmission. It turns out that up to a fourfold increase in the network throughput can be achieved with practical numbers of modulation levels in comparison to the traditionally considered binary case. Finally, we present BER simulation results for channel coded binary and[InlineEquation not available: see fulltext.]-ary OCDMA transmission systems. In particular, we apply turbo codes concatenated with Reed-Solomon codes so that up to several hundred concurrent optical CDMA users can be supported at low target bit error rates. We observe that unlike conventional OCDMA systems, turbo-empowered OCDMA can allow overloading (more active users than is the length of the spreading sequences) with good bit error rate system performance.

Enhanced noise tolerance for 10 Gb/s Bi-directional cross-wavelength reuse colorless WDM-PON by using spectrally shaped OFDM signals

NASA Astrophysics Data System (ADS)

Choudhury, Pallab K.

2018-05-01

Spectrally shaped orthogonal frequency division multiplexing (OFDM) signal for symmetric 10 Gb/s cross-wavelength reuse reflective semiconductor optical amplifier (RSOA) based colorless wavelength division multiplexed passive optical network (WDM-PON) is proposed and further analyzed to support broadband services of next generation high speed optical access networks. The generated OFDM signal has subcarriers in separate frequency ranges for downstream and upstream, such that the re-modulation noise can be effectively minimized in upstream data receiver. Moreover, the cross wavelength reuse approach improves the tolerance against Rayleigh backscattering noise due to the propagation of different wavelengths in the same feeder fiber. The proposed WDM-PON is successfully demonstrated for 25 km fiber with 16-QAM (quadrature amplitude modulation) OFDM signal having bandwidth of 2.5 GHz for 10 Gb/s operation and subcarrier frequencies in 3-5.5 GHz and DC-2.5 GHz for downstream (DS) and upstream (US) transmission respectively. The result shows that the proposed scheme maintains a good bit error rate (BER) performance below the forward error correction (FEC) limit of 3.8 × 10-3 at acceptable receiver sensitivity and provides a high resilience against re-modulation and Rayleigh backscattering noises as well as chromatic dispersion.

Adaptive upstream rate adjustment by RSOA-ONU depending on different injection power of seeding light in standard-reach and long-reach PON systems

NASA Astrophysics Data System (ADS)

Yeh, C. H.; Chow, C. W.; Shih, F. Y.; Pan, C. L.

2012-08-01

The wavelength division multiplexing-time division multiplexing (WDM-TDM) passive optical network (PON) using reflective semiconductor optical amplifier (RSOA)-based colorless optical networking units (ONUs) is considered as a promising candidate for the realization of fiber-to-the-home (FTTH). And this architecture is actively considered by Industrial Technology Research Institute (ITRI) for the realization of FTTH in Taiwan. However, different fiber distances and optical components would introduce different power budgets to different ONUs in the PON. Besides, due to the aging of optical transmitter (Tx), the power decay of the distributed optical carrier from the central office (CO) could also reduce the injection power into each ONU. The situation will be more severe in the long-reach (LR) PON, which is considered as an option for the future access. In this work, we investigate a WDM-TDM PON using RSOA-based ONU for upstream data rate adjustment depending on different continuous wave (CW) injection powers. Both standard-reach (25 km) and LR (100 km) transmissions are evaluated. Moreover, a detail analysis of the upstream signal bit-error rate (BER) performances at different injection powers, upstream data rates, PON split-ratios under stand-reach and long-reach is presented.

Optical switch compatible with wavelength division multiplexing and mode division multiplexing for photonic networks-on-chip.

PubMed

Jia, Hao; Zhou, Ting; Zhang, Lei; Ding, Jianfeng; Fu, Xin; Yang, Lin

2017-08-21

We propose a 2 × 2 multimode optical switch, which is composed of two mode de-multiplexers, n 2 × 2 single-mode optical switches where n is the number of the supported spatial modes, and two mode multiplexers. As a proof of concept, asymmetric directional couplers are employed to construct the mode multiplexers and de-multiplexers, balanced Mach-Zehnder interferometer is utilized to construct the 2 × 2 single-mode optical switches. The fabricated silicon 2 × 2 multimode optical switch has a broad optical bandwidth and can support four spatial modes. The link-crosstalk for all four modes is smaller than -18.8 dB. The inter-mode crosstalk for the same optical link is less than -22.1 dB. 40 Gbps data transmission is performed for all spatial modes and all optical links. The power penalties for the error-free switching (BER<10 -9 ) at 25 Gbps are less than 1.8 dB for all channels at the wavelength of 1550 nm. The power consumption of the device is 117.9 mW in the "cross" state and 116.2 mW in the "bar" state. The switching time is about 21 μs. This work enables large-capacity multimode photonic networks-on-chip.

National Geographic Society Kids Network: Report on 1994 teacher participants

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

In 1994, National Geographic Society Kids Network, a computer/telecommunications-based science curriculum, was presented to elementary and middle school teachers through summer programs sponsored by NGS and US DOE. The network program assists teachers in understanding the process of doing science; understanding the role of computers and telecommunications in the study of science, math, and engineering; and utilizing computers and telecommunications appropriately in the classroom. The program enables teacher to integrate science, math, and technology with other subjects with the ultimate goal of encouraging students of all abilities to pursue careers in science/math/engineering. This report assesses the impact of the networkmore » program on participating teachers.« less

Low Temperature Plasma for the Treatment of Epithelial Cancer Cells

NASA Astrophysics Data System (ADS)

Mohades, Soheila

Biomedical applications of low temperature plasmas (LTP) may lead to a paradigm shift in treating various diseases by conducting fundamental research on the effects of LTP on cells, tissues, organisms (plants, insects, and microorganisms). This is a rapidly growing interdisciplinary research field that involves engineering, physics, life sciences, and chemistry to find novel solutions for urgent medical needs. Effects of different LTP sources have shown the anti-tumor properties of plasma exposure; however, there are still many unknowns about the interaction of plasma with eukaryotic cells which must be elucidated in order to evaluate the practical potential of plasma in cancer treatment. Plasma, the fourth state of matter, is composed of electrons, ions, reactive molecules (radicals and non-radicals), excited species, radiation, and heat. A sufficient dose (time) of plasma exposure can induce death in cancer cells. The plasma pencil is employed to study the anti-tumor properties of this treatment on epithelial cells. The plasma pencil has been previously used for the inactivation of bacteria, destroying amyloid fibrils, and the killing of various cancer cells. Bladder cancer is the 9th leading cause of cancer. In this dissertation, human urinary bladder tissue with the squamous cell carcinoma disease (SCaBER cells) is treated with LTP utilizing two different approaches: direct plasma exposure and Plasma Activated Media (PAM) as an advancement to the treatment. PAM is produced by exposing a liquid cell culture medium to the plasma pencil. Direct LTP treatment of cancer cells indicates a dose-dependent killing effect at post-treatment times. Similarly, PAM treatment shows an anti-cancer effect by inducing substantial cell death. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) have an important role in the biomedical effects of LTP treatment. This study demonstrates the capability of the plasma pencil to transport ROS/RNS into cell culture media leading to their activation. The effectiveness of PAM against SCaBER cells is the highest when it is used immediately after preparation. It is found that the killing effect of PAM decreases gradually over time, depending on the dose of plasma exposure. Hydrogen peroxide is known as one of the most stable and impactful ROS in biological systems. Measurements show that the plasma pencil generates a significant amount of hydrogen peroxide in PAM. Interestingly, the concentration of hydrogen peroxide in PAM decreases gradually over time, which correlates well with the decrease of PAM effectiveness with storage time. While the effects of PAM treatment on cancerous epithelial cell lines have been studied, much less is known about the interaction of PAM with normal epithelial cells. Effects of PAM on non-cancerous Madin-Darby Canine kidney (MDCK) epithelial cells indicates that MDCK cells are much more robust than SCaBER cells against PAM treatment. The dose of PAM, which causes a widespread death in SCaBER cells, does not significantly impact viability and morphology of MDCK cells. Time-lapse imaging of normal cells shows that PAM treatment inhibits cell proliferation and random migration. In addition, immunofluorescence staining shows that PAM treatment causes a significant reduction in the nuclear localization of proliferation marker, Ki-67, without any damage to the morphological properties of cells including adhesions and cytoskeleton function. This dissertation clearly demonstrates the capability of PAM treatment in inducing death in cancerous cells that can be important for cancer therapy. Hydrogen peroxide is identified as an important ROS responsible for the anti-tumor properties of PAM, although much additional work remains to comprehensively understand all the involved ROS/RNS and their role in PAM treatment.

Optimizing available network resources to address questions in environmental biogeochemistry

USGS Publications Warehouse

Hinckley, Eve-Lyn; Suzanne Andersen,; Baron, Jill S.; Peter Blanken,; Gordon Bonan,; William Bowman,; Sarah Elmendorf,; Fierer, Noah; Andrew Fox,; Keli Goodman,; Katherine Jones,; Danica Lombardozzi,; Claire Lunch,; Jason Neff,; Michael SanClements,; Katherine Suding,; Will Wieder,

2016-01-01

An increasing number of network observatories have been established globally to collect long-term biogeochemical data at multiple spatial and temporal scales. Although many outstanding questions in biogeochemistry would benefit from network science, the ability of the earth- and environmental-sciences community to conduct synthesis studies within and across networks is limited and seldom done satisfactorily. We identify the ideal characteristics of networks, common problems with using data, and key improvements to strengthen intra- and internetwork compatibility. We suggest that targeted improvements to existing networks should include promoting standardization in data collection, developing incentives to promote rapid data release to the public, and increasing the ability of investigators to conduct their own studies across sites. Internetwork efforts should include identifying a standard measurement suite—we propose profiles of plant canopy and soil properties—and an online, searchable data portal that connects network, investigator-led, and citizen-science projects.

Computer Networking Strategies for Building Collaboration among Science Educators.

ERIC Educational Resources Information Center

Aust, Ronald

The development and dissemination of science materials can be associated with technical delivery systems such as the Unified Network for Informatics in Teacher Education (UNITE). The UNITE project was designed to investigate ways for using computer networking to improve communications and collaboration among university schools of education and…

Networked Environments that Create Hybrid Spaces for Learning Science

ERIC Educational Resources Information Center

Otrel-Cass, Kathrin; Khoo, Elaine; Cowie, Bronwen

2014-01-01

Networked learning environments that embed the essence of the Community of Inquiry (CoI) framework utilise pedagogies that encourage dialogic practices. This can be of significance for classroom teaching across all curriculum areas. In science education, networked environments are thought to support student investigations of scientific problems,…

8-Oxoguanine DNA glycosylase1-driven DNA repair-A paradoxical role in lung aging.

PubMed

German, Peter; Saenz, David; Szaniszlo, Peter; Aguilera-Aguirre, Leopoldo; Pan, Lang; Hegde, Muralidhar L; Bacsi, Attila; Hajas, Gyorgy; Radak, Zsolt; Ba, Xueqing; Mitra, Sankar; Papaconstantinou, John; Boldogh, Istvan

2017-01-01

Age-associated changes in lung structure and function are some of the most important predictors of overall health, cognitive activities and longevity. Common to all aging cells is an increase in oxidatively modified DNA bases, primarily 8-oxo-7,8-dihydroguanine (8-oxoG). It is repaired via DNA base excision repair pathway driven by 8-oxoguanine DNA glycosylase-1 (OGG1-BER), whose role in aging has been the focus of many studies. This study hypothesizes that signaling and consequent gene expression during cellular response to OGG1-BER "wires" senescence/aging processes. To test OGG1-BER was mimicked by repeatedly exposing diploid lung fibroblasts cells and airways of mice to 8-oxoG base. Results showed that repeated exposures led to G1 cell cycle arrest and pre-matured senescence of cultured cells in which over 1000 genes were differentially expressed -86% of them been identical to those in naturally senesced cells. Gene ontology analysis of gene expression displayed biological processes driven by small GTPases, phosphoinositide 3-kinase and mitogen activated kinase cascades both in cultured cells and lungs. These results together, points to a new paradigm about the role of DNA damage and repair by OGG1 in aging and age-associated disease processes. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Distributed polar-coded OFDM based on Plotkin's construction for half duplex wireless communication

NASA Astrophysics Data System (ADS)

Umar, Rahim; Yang, Fengfan; Mughal, Shoaib; Xu, HongJun

2018-07-01

A Plotkin-based polar-coded orthogonal frequency division multiplexing (P-PC-OFDM) scheme is proposed and its bit error rate (BER) performance over additive white gaussian noise (AWGN), frequency selective Rayleigh, Rician and Nakagami-m fading channels has been evaluated. The considered Plotkin's construction possesses a parallel split in its structure, which motivated us to extend the proposed P-PC-OFDM scheme in a coded cooperative scenario. As the relay's effective collaboration has always been pivotal in the design of cooperative communication therefore, an efficient selection criterion for choosing the information bits has been inculcated at the relay node. To assess the BER performance of the proposed cooperative scheme, we have also upgraded conventional polar-coded cooperative scheme in the context of OFDM as an appropriate bench marker. The Monte Carlo simulated results revealed that the proposed Plotkin-based polar-coded cooperative OFDM scheme convincingly outperforms the conventional polar-coded cooperative OFDM scheme by 0.5 0.6 dBs over AWGN channel. This prominent gain in BER performance is made possible due to the bit-selection criteria and the joint successive cancellation decoding adopted at the relay and the destination nodes, respectively. Furthermore, the proposed coded cooperative schemes outperform their corresponding non-cooperative schemes by a gain of 1 dB under an identical condition.

„3D-augmented-reality“-Visualisierung für die navigierte Osteosynthese von Beckenfrakturen

PubMed Central

Befrui, N.; Fischer, M.; Fuerst, B.; Lee, S.-C.; Fotouhi, J.; Weidert, S.; Johnson, A.; Euler, E.; Osgood, G.; Navab, N.; Böcker, W.

2018-01-01

Zusammenfassung Hintergrund Trotz großer Fortschritte in der Entwicklung der Hard- und Software von Navigationssystemen finden diese aufgrund ihrer vermeintlichen Komplexität, umständlichen Integration in klinische Arbeitsabläufe und fraglichen Vorteilen gegenüber konventionellen bildgebenden Verfahren bisher wenig Einsatz in den heutigen Operationssälen. Ziel der Arbeit Entwicklung einer „Augmented-reality“(AR)-Darstellung zur chirurgischen Navigation ohne Infrarot(„IR“)-Tracking-Marker und Vergleich zum konventioneller Röntgen in einem simulierten Eingriff. Material und Methoden Navigationssystem bestehend aus „Cone-beam-CT“(CBCT)-fähigem C-Bogen und „Red-green-blue-depth“(RGBD)-Kamera. Testung durch Kirschner(K)-Draht-Platzierung in Modellen unter Berücksichtigung der benötigten Zeit, der Strahlendosis und der Benutzerfreundlichkeit der Systeme. Ergebnisse Eine signifikante Reduktion der benötigten Zeit, der Röntgenbilder und der gesamten Strahlendosis bei der AR-Navigation gegenüber dem konventionellen Röntgen bei gleichbleibender Präzision. Schlussfolgerung Die AR-Navigation mithilfe der RGBD-Kamera bietet flexible und intuitive Darstellungsmöglichkeiten des Operations-situs für navigierte Osteosynthesen ohne Tracking-Marker. Hiermit ist es möglich, Operationen schneller, einfacher und mit geringerer Strahlenbelastung für Patient und OP-Personal durchzuführen. PMID:29500506

Confidence Intervals for Error Rates Observed in Coded Communications Systems

NASA Astrophysics Data System (ADS)

Hamkins, J.

2015-05-01

We present methods to compute confidence intervals for the codeword error rate (CWER) and bit error rate (BER) of a coded communications link. We review several methods to compute exact and approximate confidence intervals for the CWER, and specifically consider the situation in which the true CWER is so low that only a handful, if any, codeword errors are able to be simulated. In doing so, we answer the question of how long an error-free simulation must be run in order to certify that a given CWER requirement is met with a given level of confidence, and discuss the bias introduced by aborting a simulation after observing the first codeword error. Next, we turn to the lesser studied problem of determining confidence intervals for the BER of coded systems. Since bit errors in systems that use coding or higher-order modulation do not occur independently, blind application of a method that assumes independence leads to inappropriately narrow confidence intervals. We present a new method to compute the confidence interval properly, using the first and second sample moments of the number of bit errors per codeword. This is the first method we know of to compute a confidence interval for the BER of a coded or higher-order modulation system.

Equalization for a page-oriented optical memory system

NASA Astrophysics Data System (ADS)

Trelewicz, Jennifer Q.; Capone, Jeffrey

1999-11-01

In this work, a method of decision-feedback equalization is developed for a digital holographic channel that experiences moderate-to-severe imaging errors. Decision feedback is utilized, not only where the channel is well-behaved, but also near the edges of the camera grid that are subject to a high degree of imaging error. In addition to these effects, the channel is worsened by typical problems of holographic channels, including non-uniform illumination, dropouts, and stuck bits. The approach described in this paper builds on established methods for performing trained and blind equalization on time-varying channels. The approach is tested on experimental data sets. On most of these data sets, the method of equalization described in this work delivers at least an order of magnitude improvement in bit-error rate (BER) before error-correction coding (ECC). When ECC is introduced, the approach is able to recover stored data with no errors for many of the tested data sets. Furthermore, a low BER was maintained even over a range of small alignment perturbations in the system. It is believed that this equalization method can allow cost reductions to be made in page-memory systems, by allowing for a larger image area per page or less complex imaging components, without sacrificing the low BER required by data storage applications.

The PARP inhibitor ABT-888 potentiates dacarbazine-induced cell death in carcinoids.

PubMed

Somnay, Y; Lubner, S; Gill, H; Matsumura, J B; Chen, H

2016-10-01

Monoagent DNA-alkylating chemotherapies like dacarbazine are among a paucity of medical treatments for advanced carcinoid tumors, but are limited by host toxicity and intrinsic chemoresistance through the base excision repair (BER) pathway via poly (ADP-ribose) polymerase (PARP). Hence, inhibitors of PARP may potentiate DNA-damaging agents by blocking BER and DNA restoration. We show that the PARP inhibitor ABT-888 (Veliparib) enhances the cytotoxic effects of dacarbazine in carcinoids. Two human carcinoid cell lines (BON and H727) treated with a combination of ABT-888 and dacarbazine resulted in synergistic growth inhibition signified by combination indices <1 on the Chou-Talalay scale. ABT-888 administered prior to varying dacarbazine doses promoted the suppression of neuroendocrine biomarkers of malignancy, ASCL1 and chromogranin A, as shown by western analysis. Ataxia telangiectasia mitogen factor phosphorylation and p21 Waf1/Cip1 activation, indicative of DNA damage, were increased by ABT-888 when combined with dacarbazine treatment, suggesting BER pathway attenuation by ABT-888. PE Annexin V/7-AAD staining and sorting revealed a profound induction of apoptosis following combination treatment, which was further confirmed by increased PARP cleavage. These results demonstrate that ABT-888 synergizes dacarbazine treatment in carcinoids. Therefore, ABT-888 may help treat carcinoids unresponsive or refractory to mainstay therapies.

Application of supercritical water to decompose brominated epoxy resin and environmental friendly recovery of metals from waste memory module.

PubMed

Li, Kuo; Xu, Zhenming

2015-02-03

Waste Memory Modules (WMMs), a particular kind of waste printed circuit board (WPCB), contain a high amount of brominated epoxy resin (BER), which may bring a series of environmental and health problems. On the other hand, metals like gold and copper are very valuable and are important to recover from WMMs. In the present study, an effective and environmental friendly method using supercritical water (SCW) to decompose BER and recover metals from WMMs was developed instead of hydrometallurgy or pyrometallurgy simultaneously. Experiments were conducted under external-catalyst-free conditions with temperatures ranging from 350 to 550 °C, pressures from 25 to 40 MPa, and reaction times from 120 to 360 min in a semibatch-type reactor. The results showed that BER could be quickly and efficiently decomposed under SCW condition, and the mechanism was possibly free radical reaction. After the SCW treatments, the glass fibers and metal foils in the solid residue could be easily liberated and recovered, respectively. The metal recovery rate reached 99.80%. The optimal parameters were determined as 495 °C, 33 MPa, and 305 min on the basis of response surface methodology (RSM). This study provides an efficient and environmental friendly approach for WMMs recycling compared with electrolysis, pyrometallurgy, and hydrometallurgy.

Performance Enhancement of MC-CDMA System through Novel Sensitive Bit Algorithm Aided Turbo Multi User Detection

PubMed Central

Kumaravel, Rasadurai; Narayanaswamy, Kumaratharan

2015-01-01

Multi carrier code division multiple access (MC-CDMA) system is a promising multi carrier modulation (MCM) technique for high data rate wireless communication over frequency selective fading channels. MC-CDMA system is a combination of code division multiple access (CDMA) and orthogonal frequency division multiplexing (OFDM). The OFDM parts reduce multipath fading and inter symbol interference (ISI) and the CDMA part increases spectrum utilization. Advantages of this technique are its robustness in case of multipath propagation and improve security with the minimize ISI. Nevertheless, due to the loss of orthogonality at the receiver in a mobile environment, the multiple access interference (MAI) appears. The MAI is one of the factors that degrade the bit error rate (BER) performance of MC-CDMA system. The multiuser detection (MUD) and turbo coding are the two dominant techniques for enhancing the performance of the MC-CDMA systems in terms of BER as a solution of overcome to MAI effects. In this paper a low complexity iterative soft sensitive bits algorithm (SBA) aided logarithmic-Maximum a-Posteriori algorithm (Log MAP) based turbo MUD is proposed. Simulation results show that the proposed method provides better BER performance with low complexity decoding, by mitigating the detrimental effects of MAI. PMID:25714917

Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prakash, Aishwarya; Moharana, Kedar; Wallace, Susan S.

The base excision repair (BER) pathway repairs oxidized lesions in the DNA that result from reactive oxygen species generated in cells. If left unrepaired, these damaged DNA bases can disrupt cellular processes such as replication. NEIL1 is one of the 11 human DNA glycosylases that catalyze the first step of the BER pathway, i.e. recognition and excision of DNA lesions. NEIL1 interacts with essential replication proteins such as the ring-shaped homotrimeric proliferating cellular nuclear antigen (PCNA). We isolated a complex formed between NEIL1 and PCNA (±DNA) using size exclusion chromatography (SEC). This interaction was confirmed using native gel electrophoresis andmore » mass spectrometry. Stokes radii measured by SEC hinted that PCNA in complex with NEIL1 (±DNA) was no longer a trimer. Height measurements and images obtained by atomic force microscopy also demonstrated the dissociation of the PCNA homotrimer in the presence of NEIL1 and DNA, while small-angle X-ray scattering analysis confirmed the NEIL1 mediated PCNA trimer dissociation and formation of a 1:1:1 NEIL1-DNA-PCNA(monomer) complex. Furthermore, ab initio shape reconstruction provides insights into the solution structure of this previously unreported complex. Together, these data point to a potential mechanistic switch between replication and BER.« less

Nematodes as bioindicators of ecosystem recovery during phytoremediation of crude oil contaminated soil.

PubMed

Savin, Mary C; Wolf, Duane C; Davis, K Jody; Gbur, Edward E; Thoma, Greg J

2015-01-01

Restoration of a weathered crude oil contaminated site undergoing phytoremediation was evaluated using nematodes as bioindicators. Samples were collected twice per year equating to spring and fall/winter. Mean annual total abundances ranged from 18-130 in the non-fertilized non-vegetated control (CTR) to 69-728 in tall fescue-ryegrass (FES) to 147-749 (100 g(-1)) in the fertilized bermudagrass-fescue (BER) treatment. Proportions of plant-parasitic (PP) and free-living (FL) nematodes were significantly impacted by treatment, but not year, with PP nematodes accounting for 27, 59, and 68% of CTR, FES, and BER communities, respectively. There was no significant year by season by treatment or treatment by year effect for total, PP, or FL nematode abundances. Diversity did not increase over time. The BER and FES treatments had more mature communities as indicated by higher plant-parasitic index (PPI) values. Phytoremediation accelerates petroleum degradation and alters the soil habitat which is reflected in the nematode community. However, low numbers and inconsistent presence of persister strategist omnivores and predators, and the lack in improvement over time in treatment effects for total and PP nematode abundances, PP and FL proportions, or PPI indicate the system is being rehabilitated but has not been restored after 69 months of phytoremediation.

Uptake of 2S albumin allergens, Ber e 1 and Ses i 1, across human intestinal epithelial Caco-2 cell monolayers.

PubMed

Moreno, F Javier; Rubio, Luis A; Olano, Agustín; Clemente, Alfonso

2006-11-01

We have investigated the absorption rates of two purified major allergen 2S albumins, Ber e 1 from Brazil nuts (Bertholletia excelsa Humb. & Bonpl.) and Ses i 1 from white sesame seeds (Sesamum indicum L.), across human intestinal epithelial Caco-2 cell monolayers following gastrointestinal digestion in vitro. The transport from apical to basolateral side in cell monolayers was evaluated by RP-HPLC-UV and indirect competitive ELISA methods, being confirmed by western-blotting analysis. Significant amounts (approximately 15-25 nmol micromol(-1) initial amount/h) of intact Ber e 1 and Ses i 1 were found in the basolateral side. The absorption rates of both plant allergens through the cell monolayer were shown to be constant during the whole incubation period (4 h at 37 degrees C), verifying that the permeability of the membrane was not altered by the allergen digests. Our findings revealed that both purified 2S albumin allergens may be able to survive in immunologically reactive forms to the simulated harsh conditions of the gastrointestinal tract to be transported across the Caco-2 cell monolayers, so that they would be able to sensitize the mucosal immune system and/or elicit an allergic response.

Evidence for a Role of FEN1 in Maintaining Mitochondrial DNA Integrity

PubMed Central

Kalifa, Lidza; Beutner, Gisela; Phadnis, Naina; Sheu, Shey-Shing; Sia, Elaine A.

2009-01-01

Although the nuclear processes responsible for genomic DNA replication and repair are well characterized, the pathways involved in mitochondrial DNA (mtDNA) replication and repair remain unclear. DNA repair has been identified as being particularly important within the mitochondrial compartment due to the organelle’s high propensity to accumulate oxidative DNA damage. It has been postulated that continual accumulation of mtDNA damage and subsequent mutagenesis may function in cellular aging. Mitochondrial base excision repair (mtBER) plays a major role in combating mtDNA oxidative damage; however, the proteins involved in mtBER have yet to be fully characterized. It has been established that during nuclear long-patch (LP) BER, FEN1 is responsible for cleavage of 5′ flap structures generated during DNA synthesis. Furthermore, removal of 5′ flaps has been observed in mitochondrial extracts of mammalian cell lines; yet, the mitochondrial localization of FEN1 has not been clearly demonstrated. In this study, we analyzed the effects of deleting the yeast FEN1 homolog, RAD27, on mtDNA stability in Saccharomyces cerevisiae. Our findings demonstrate that Rad27p/FEN1 is localized in the mitochondrial compartment of both yeast and mice and that Rad27p has a significant role in maintaining mtDNA integrity. PMID:19699691

Mechanisms of chemoresistance to alkylating agents in malignant glioma.

PubMed

Sarkaria, Jann N; Kitange, Gaspar J; James, C David; Plummer, Ruth; Calvert, Hilary; Weller, Michael; Wick, Wolfgang

2008-05-15

Intrinsic or acquired chemoresistance to alkylating agents is a major cause of treatment failure in patients with malignant brain tumors. Alkylating agents, the mainstay of treatment for brain tumors, damage the DNA and induce apoptosis, but the cytotoxic activity of these agents is dependent on DNA repair pathways. For example, O6-methylguanine DNA adducts can cause double-strand breaks, but this is dependent on a functional mismatch repair pathway. Thus, tumor cell lines deficient in mismatch repair are resistant to alkylating agents. Perhaps the most important mechanism of resistance to alkylating agents is the DNA repair enzyme O6-methylguanine methyltransferase, which can eliminate the cytotoxic O6-methylguanine DNA adduct before it causes harm. Another mechanism of resistance to alkylating agents is the base excision repair (BER) pathway. Consequently, efforts are ongoing to develop effective inhibitors of BER. Poly(ADP-ribose)polymerase plays a pivotal role in BER and is an important therapeutic target. Developing effective strategies to overcome chemoresistance requires the identification of reliable preclinical models that recapitulate human disease and which can be used to facilitate drug development. This article describes the diverse mechanisms of chemoresistance operating in malignant glioma and efforts to develop reliable preclinical models and novel pharmacologic approaches to overcome resistance to alkylating agents.

Close to 100 Gbps discrete multitone transmission over 100m of multimode fiber using a single transverse mode 850nm VCSEL

NASA Astrophysics Data System (ADS)

Wu, Bo; Zhou, Xian; Ma, Yanan; Luo, Jun; Zhong, Kangping; Qiu, Shaofeng; Feng, Zhiyong; Luo, Yazhi; Agustin, Mikel; Ledentsov, Nikolay; Kropp, Joerg; Shchukin, Vitaly; Ledentsov, Nikolay N.; Eddie, Iain; Chao, Lu

2016-03-01

Discrete Multitone Transmission (DMT) transmission over standard multimode fiber (MMF) using high-speed single (SM) and multimode (MM) Vertical-Cavity Surface-Emitting Lasers (VCSELs) is studied. Transmission speed in the range of 72Gbps to 82Gbps over 300m -100m distances of OM4 fiber is realized, respectively, at Bit-Error-Ratio (BER) <5e-3 and the received optical power of only -5dBm. Such BER condition requires only 7% overhead for the conversion to error-free operation using single Bose-Chaudhuri-Hocquenghem forward error correction (BCH-FEC) coding and decoding. SM VCSEL is demonstrated to provide a much higher data transmission capacity over MMF. For 100m MMF transmission SM VCSEL allows 82Gbps as compared to MM VCSEL resulting in only 34Gbps at the same power (-5dBm). Furthermore, MM VCSEL link at 0dBm is still restricted at 100m distance by 63Gbps while SM VCSEL can exceed 100Gbps at such power levels. We believe that with further improvement in SM VCSELs and fiber coupling >100Gbps data transmission over >300m MMF distances at the BER levels matching the industry standards will become possible.

Probabilistic QoS Analysis In Wireless Sensor Networks

DTIC Science & Technology

2012-04-01

and A.O. Fapojuwo. TDMA scheduling with optimized energy efficiency and minimum delay in clustered wireless sensor networks . IEEE Trans. on Mobile...Research Computer Science and Engineering, Department of 5-1-2012 Probabilistic QoS Analysis in Wireless Sensor Networks Yunbo Wang University of...Wang, Yunbo, "Probabilistic QoS Analysis in Wireless Sensor Networks " (2012). Computer Science and Engineering: Theses, Dissertations, and Student

Asian Network for Biological Sciences (ANBS).

ERIC Educational Resources Information Center

Asian Network for Biological Sciences.

The Asian Network for Biological Sciences (ANBS) is a group of institutions, laboratories, research centers, and scholars who are willing to cooperate in programs and activities aimed at improving teaching and research in the biological sciences. This publication: (1) outlines ANBS aims and objectives; (2) describes major activities in the past;…

Nuclear Physics Science Network Requirements Workshop, May 2008 - Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tierney, Ed., Brian L; Dart, Ed., Eli; Carlson, Rich

2008-11-10

The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the US Department of Energy Office of Science, the single largest supporter of basic research in the physical sciences in the United States of America. In support of the Office of Science programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 20 years. In May 2008, ESnet and the Nuclear Physics (NP) Program Office of the DOEmore » Office of Science organized a workshop to characterize the networking requirements of the science programs funded by the NP Program Office. Most of the key DOE sites for NP related work will require significant increases in network bandwidth in the 5 year time frame. This includes roughly 40 Gbps for BNL, and 20 Gbps for NERSC. Total transatlantic requirements are on the order of 40 Gbps, and transpacific requirements are on the order of 30 Gbps. Other key sites are Vanderbilt University and MIT, which will need on the order of 20 Gbps bandwidth to support data transfers for the CMS Heavy Ion program. In addition to bandwidth requirements, the workshop emphasized several points in regard to science process and collaboration. One key point is the heavy reliance on Grid tools and infrastructure (both PKI and tools such as GridFTP) by the NP community. The reliance on Grid software is expected to increase in the future. Therefore, continued development and support of Grid software is very important to the NP science community. Another key finding is that scientific productivity is greatly enhanced by easy researcher-local access to instrument data. This is driving the creation of distributed repositories for instrument data at collaborating institutions, along with a corresponding increase in demand for network-based data transfers and the tools to manage those transfers effectively. Network reliability is also becoming more important as there is often a narrow window between data collection and data archiving when transfer and analysis can be done. The instruments do not stop producing data, so extended network outages can result in data loss due to analysis pipeline stalls. Finally, as the scope of collaboration continues to increase, collaboration tools such as audio and video conferencing are becoming ever more critical to the productivity of scientific collaborations.« less

Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay

PubMed Central

Nickson, Catherine M.; Parsons, Jason L.

2014-01-01

Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss, and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a consequence of cellular metabolism and exogenous agents, including ionizing radiation. Over the last three decades the comet assay has been employed in scientific research to examine the cellular response to these types of DNA damage in cultured cells, therefore revealing the efficiency and capacity of BER. We have recently pioneered new research demonstrating an important role for post-translational modifications (particularly ubiquitylation) in the regulation of cellular levels of BER proteins, and that subtle changes (∼20–50%) in protein levels following siRNA knockdown of E3 ubiquitin ligases or deubiquitylation enzymes can manifest in significant changes in DNA repair capacity monitored using the comet assay. For example, we have shown that the E3 ubiquitin ligase Mule, the tumor suppressor protein ARF, and the deubiquitylation enzyme USP47 modulate DNA repair by controlling cellular levels of DNA polymerase β, and also that polynucleotide kinase phosphatase levels are controlled by ATM-dependant phosphorylation and Cul4A–DDB1–STRAP-dependent ubiquitylation. In these studies we employed a modification of the comet assay whereby cultured cells, following DNA damage treatment, are embedded in agarose and allowed to repair in-gel prior to lysis and electrophoresis. Whilst this method does have its limitations, it avoids the extensive cell culture-based processing associated with the traditional approach using attached cells and also allows for the examination of much more precise DNA repair kinetics. In this review we will describe, using this modified comet assay, our accumulating evidence that ubiquitylation-dependant regulation of BER proteins has important consequences for overall cellular DNA repair capacity. PMID:25076968

An oxidative DNA “damage” and repair mechanism localized in the VEGF promoter is important for hypoxia-induced VEGF mRNA expression

PubMed Central

Pastukh, Viktor; Roberts, Justin T.; Clark, David W.; Bardwell, Gina C.; Patel, Mita; Al-Mehdi, Abu-Bakr; Borchert, Glen M.

2015-01-01

In hypoxia, mitochondria-generated reactive oxygen species not only stimulate accumulation of the transcriptional regulator of hypoxic gene expression, hypoxia inducible factor-1 (Hif-1), but also cause oxidative base modifications in hypoxic response elements (HREs) of hypoxia-inducible genes. When the hypoxia-induced base modifications are suppressed, Hif-1 fails to associate with the HRE of the VEGF promoter, and VEGF mRNA accumulation is blunted. The mechanism linking base modifications to transcription is unknown. Here we determined whether recruitment of base excision DNA repair (BER) enzymes in response to hypoxia-induced promoter modifications was required for transcription complex assembly and VEGF mRNA expression. Using chromatin immunoprecipitation analyses in pulmonary artery endothelial cells, we found that hypoxia-mediated formation of the base oxidation product 8-oxoguanine (8-oxoG) in VEGF HREs was temporally associated with binding of Hif-1α and the BER enzymes 8-oxoguanine glycosylase 1 (Ogg1) and redox effector factor-1 (Ref-1)/apurinic/apyrimidinic endonuclease 1 (Ape1) and introduction of DNA strand breaks. Hif-1α colocalized with HRE sequences harboring Ref-1/Ape1, but not Ogg1. Inhibition of BER by small interfering RNA-mediated reduction in Ogg1 augmented hypoxia-induced 8-oxoG accumulation and attenuated Hif-1α and Ref-1/Ape1 binding to VEGF HRE sequences and blunted VEGF mRNA expression. Chromatin immunoprecipitation-sequence analysis of 8-oxoG distribution in hypoxic pulmonary artery endothelial cells showed that most of the oxidized base was localized to promoters with virtually no overlap between normoxic and hypoxic data sets. Transcription of genes whose promoters lost 8-oxoG during hypoxia was reduced, while those gaining 8-oxoG was elevated. Collectively, these findings suggest that the BER pathway links hypoxia-induced introduction of oxidative DNA modifications in promoters of hypoxia-inducible genes to transcriptional activation. PMID:26432868