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Sample records for biochemistry buenos aires

  1. [Past and present of Medicina (Buenos Aires)].

    PubMed

    Pasqualini, C D; Kotsias, B A

    2000-01-01

    To celebrate the 60th anniversary of Medicina (Buenos Aires) an International Symposium was held at the National Academy of Medicine of Buenos Aires on the 6-7th of October 1999, under the title of Clinical investigation in the next millennium. This meeting was a success as evidenced by the 376 registered attendants. Sixty years of uninterrupted publication is an uncommon feat in our midst and this could be achieved on the basis of a number of factors which include, the initiative of those who founded the journal, the unfailing motivation and dedication of the Editorial Board and primarily the authors who have trusted us with their manuscripts. Of the many important papers published, we have selected a few which proved to be milestones in the development of Argentine biomedicine. It is to be hoped that the future will bring an increase in our impact index through more and even better papers eventually reflecting the authentic scientific value of our country.

  2. [The seventieth anniversary of Medicina (Buenos Aires)].

    PubMed

    Kantor, Isabel N; Kotsias, Basilio A

    2010-01-01

    The historical trajectory of Medicina (Buenos Aires) and the current challenges accompanying its seventieth birthday are briefly described. The initial objectives at its foundation were to contribute to the advance of medicine, and support both clinical and experimental research in Argentina. These objectives continue to be valid. The editorial presence of the journal continues to be necessary. It is published following the international quality standards, the peer review system, and it is indexed in the main international data bases for scientific journals.

  3. A palace for astronomy in Buenos Aires

    NASA Astrophysics Data System (ADS)

    Gangui, Alejandro

    2011-06-01

    In no other epoch of Western history like in the Middle Ages, cosmology was so key an element of culture and, one way or another, the motion of the heavens ended up impregnating the literature of that time. Among the most noteworthy poets we find Dante Alighieri, who became famous for his Commedia, a monumental poem written roughly between 1307 and his death in 1321, and which the critics from 16th century onwards dubbed Divina. In this and other works, Dante pictures the cosmic image for the world, summing up the current trends of Neoplatonic and Islamic traditions. The Barolo Palace in the city of Buenos Aires is a singular combination of both astronomy and the worldview displayed in Dante's poetic masterpiece. Some links of the Palace's main architectural structure with the three realms of the Comedy have been studied in the past. In this note we consider its unique astronomical flavor, an issue which has not been sufficiently emphasized yet.

  4. A Bilingual-FLES Pilot Program in Buenos Aires

    ERIC Educational Resources Information Center

    Schanzer, George O.

    1977-01-01

    This article describes a program in Buenos Aires at the tertiary level offering certification in elementary education plus language certification. The program is bilingual (English/Spanish); courses and methods are described. (CHK)

  5. Special Education in the Province of Buenos Aires, Argentina.

    ERIC Educational Resources Information Center

    Matthews, Peter R.; Matthews, Barbara S.

    The report is based on a study of special education in Buenos Aires, Argentina. The educational structure of the province is discussed in the first section, with information on administration and policy formation. Information on general education at the preschool, primary, and adult levels is presented along with an overview of special education…

  6. [Hantavirus pulmonary syndrome in Buenos Aires, 2009-2014].

    PubMed

    Iglesias, Ayelén A; Bellomo, Carla M; Martínez, Valeria P

    2016-01-01

    Andes virus is the causative agent of hantavirus pulmonary syndrome (HPS) in Argentina and neighboring countries. In our country four different areas are affected: Northwest, Southwest, Central and Northeast, where distinct Andes virus genotypes were characterized. Three genotypes were described in Buenos Aires province (Central area): AND-Buenos Aires, AND-Lechiguanas and AND-Plata. In this work, we considered all HPS cases confirmed by ELISA and real time RT-PCR during the period 2009-2014 in Buenos Aires province. The annual distribution, fatality rate and geographic distribution were analyzed. We also analyzed the genotypes involved by RT-PCR and nucleotide sequencing. Finally we evaluated epidemiological data in order to establish the route of transmission. We analyzed 1386 suspect cases of hantavirus infection from Buenos Aires province and we confirmed 88 cases of Hantavirus Pulmonary Syndrome during 2009-2014. The overall average was 14.3 cases per year. The occurrence of a HPS outbreak was confirmed in Buenos Aires province during 2013, showing a 3 fold increase in case number compared to the annual average between 2009 and 2012, tending to normalize during 2014. The overall lethality was 25.6%, with a maximum value of 45.5% in 2011. Genotype analysis was performed in 30.7% of confirmed cases, AND-BsAs show the highest incidence, it was characterized in 72% of the studied cases. Epidemiological data and results of viral genome comparison strongly suggest person-to-person transmission in the three clusters of two cases described in our study.

  7. [Hantavirus pulmonary syndrome in Buenos Aires, 2009-2014].

    PubMed

    Iglesias, Ayelén A; Bellomo, Carla M; Martínez, Valeria P

    2016-01-01

    Andes virus is the causative agent of hantavirus pulmonary syndrome (HPS) in Argentina and neighboring countries. In our country four different areas are affected: Northwest, Southwest, Central and Northeast, where distinct Andes virus genotypes were characterized. Three genotypes were described in Buenos Aires province (Central area): AND-Buenos Aires, AND-Lechiguanas and AND-Plata. In this work, we considered all HPS cases confirmed by ELISA and real time RT-PCR during the period 2009-2014 in Buenos Aires province. The annual distribution, fatality rate and geographic distribution were analyzed. We also analyzed the genotypes involved by RT-PCR and nucleotide sequencing. Finally we evaluated epidemiological data in order to establish the route of transmission. We analyzed 1386 suspect cases of hantavirus infection from Buenos Aires province and we confirmed 88 cases of Hantavirus Pulmonary Syndrome during 2009-2014. The overall average was 14.3 cases per year. The occurrence of a HPS outbreak was confirmed in Buenos Aires province during 2013, showing a 3 fold increase in case number compared to the annual average between 2009 and 2012, tending to normalize during 2014. The overall lethality was 25.6%, with a maximum value of 45.5% in 2011. Genotype analysis was performed in 30.7% of confirmed cases, AND-BsAs show the highest incidence, it was characterized in 72% of the studied cases. Epidemiological data and results of viral genome comparison strongly suggest person-to-person transmission in the three clusters of two cases described in our study. PMID:26826986

  8. Pandemic (H1N1) 2009 Cases, Buenos Aires, Argentina

    PubMed Central

    Querci, Marcia; Marcone, Débora; Videla, Cristina; Martínez, Alfredo; Bonvehi, Pablo; Carballal, Guadalupe

    2010-01-01

    To determine clinical and virologic characteristics of pandemic (H1N1) 2009 in Buenos Aires, Argentina, we conducted real-time reverse transcription–PCR on samples from patients with influenza-like illness, June 11–30, 2009. Of 513 patients tested, 54% were positive for influenza virus subtype H1N1. Infection rate was lowest for patients ≥60 years of age. PMID:20113568

  9. An analysis of secondary pollutants in Buenos Aires City.

    PubMed

    Reich, Silvia; Magallanes, Jorge; Dawidowski, Laura; Gómez, Darío; Groselj, Neva; Zupan, Jure

    2006-08-01

    Air pollutant concentrations from a monitoring campaign in Buenos Aires City, Argentina, are used to investigate the relationships between ambient levels of ozone (O3), nitric oxide (NO) and nitrogen dioxide (NO2) as a function of NO(x) (= NO + NO2). This campaign undertaken by the electricity sector was aimed at elucidating the apportionment of thermal power plants to air quality deterioration. Concentrations of carbon monoxide (CO) and sulphur dioxide (SO2) were also registered. Photo stationary state (PSS) of the NO, NO2, O3 and peroxy radicals species has been analysed. The 'oxidant' level concept has been introduced, OX (= O3 + NO2), which varies with the level of NO(x). It is shown that this level is made up of NO(x)-independent and NO(x)-dependent contributions. The former is a regional contribution that equates the background O3 level, whereas the latter is a local contribution that correlates with the level of primary pollution. Furthermore, the anticorrelation between NO2 and O3 levels, which is a characteristic of the atmospheric photo stationary cycle has been verified. The analysis of the concentration of the primary pollutants CO and NO strongly suggests that the vehicle traffic is the principal source of them. Levels of continuous measurements of SO2 for Buenos Aires City are reported in this work as a complement of previously published results.

  10. The Crowding Out Effect in Education: The Case of Buenos Aires in the Nineteenth Century.

    ERIC Educational Resources Information Center

    Newland, Carlos

    1994-01-01

    The privatization of public schools in Buenos Aires during the mid-1800s provides a case to test the functioning of educational markets and the relationship between private and public education supply. Private education in Buenos Aires developed within a favorable framework from 1820 to 1860. Privatization produced a competitive environment that…

  11. Second Line of Defense, Port of Buenos Aires and Exolgan Container Terminal Operational Testing and Evaluation Plan, Buenos Aires, Argentina

    SciTech Connect

    Roberts, Bryan W.

    2012-08-23

    The Office of the Second Line of Defense (SLD) Megaports project team for Argentina will conduct operational testing and evaluation (OT&E) at Exolgan Container Terminal at the Port of Dock Sud from July 16-20, 2012; and at the Port of Buenos Aires from September 3-7, 2012. SLD is installing radiation detection equipment to screen export, import, and transshipment containers at these locations. The purpose of OT&E is to validate and baseline an operable system that meets the SLD mission and to ensure the system continues to perform as expected in an operational environment with Argentina Customs effectively adjudicating alarms.

  12. Melanie Klein in Buenos Aires: beginnings and developments.

    PubMed

    Etchegoyen, R Horacio; Zysman, Samuel

    2005-06-01

    In the first decades of the 20th century, Freud was known and quoted in Latin America by an elite of enlightened minds. In the 1940s a convergence took place in Buenos Aires of European exiles with local pioneers, and thus the Argentine Psychoanalytical Association was founded in 1942. Since then psychoanalysis has grown steadily and has spread into hospitals and universities, influencing culture at large. The socioeconomic situation of that time permitted this phenomenon to develop, to the astonishment of observers. In this paper the authors study the strong influence of Kleinian thought during the first 30 years of this development. The original works of local thinkers constitute the intellectual capital that sustains the idea of an 'Argentine psychoanalytic school'. During the 1970s, both society and psychoanalysis endured deep and complex changes. Lacan's teachings gained support and Klein's influence began to decline. At present the Buenos Aires Kleinians keep working, while their relationship with Lacanians and other schools is calmer. Respectful discussions became thus possible, oriented to strictly scientific differences.

  13. [Toxic fungi in Buenos Aires City and surroundings].

    PubMed

    Romano, Gonzalo M; Iannone, Leopoldo; Novas, María V; Carmarán, Cecilia; Romero, Andrea I; López, Silvia E; Lechner, Bernardo E

    2013-01-01

    In Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires there is a service called Servicio de Identificación de Hongos Tóxicos, directed by researchers of the Program of Medicinal Plants and Fungi Involved in Biological Degradation (PROPLAME-PRHIDEB, CONICET) that assist hospitals and other health establishments, identifying the different samples of fungi and providing information about their toxicity, so that patients can receive the correct treatment. The objective of the present study was to analyze all the cases received from 1985 to 2012. This analysis permitted the confection of a table identifying the most common toxic species. The information gathered revealed that 47% of the patients were under 18 years of age and had eaten basidiomes; the remaining 53% were adults who insisted that they were able to distinguish edible from toxic mushrooms. Chlorophyllum molybdites turned out to be the main cause of fungal intoxication in Buenos Aires, which is commonly confused with Macrolepiota procera, an edible mushroom. In the second place Amanita phalloides was registered, an agaric known to cause severe symptoms after a long period of latency (6-10 hours), and which can lead to hepatic failure even requiring a transplant to prevent severe internal injuries or even death, is not early and correctly treated.

  14. [Toxic fungi in Buenos Aires City and surroundings].

    PubMed

    Romano, Gonzalo M; Iannone, Leopoldo; Novas, María V; Carmarán, Cecilia; Romero, Andrea I; López, Silvia E; Lechner, Bernardo E

    2013-01-01

    In Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires there is a service called Servicio de Identificación de Hongos Tóxicos, directed by researchers of the Program of Medicinal Plants and Fungi Involved in Biological Degradation (PROPLAME-PRHIDEB, CONICET) that assist hospitals and other health establishments, identifying the different samples of fungi and providing information about their toxicity, so that patients can receive the correct treatment. The objective of the present study was to analyze all the cases received from 1985 to 2012. This analysis permitted the confection of a table identifying the most common toxic species. The information gathered revealed that 47% of the patients were under 18 years of age and had eaten basidiomes; the remaining 53% were adults who insisted that they were able to distinguish edible from toxic mushrooms. Chlorophyllum molybdites turned out to be the main cause of fungal intoxication in Buenos Aires, which is commonly confused with Macrolepiota procera, an edible mushroom. In the second place Amanita phalloides was registered, an agaric known to cause severe symptoms after a long period of latency (6-10 hours), and which can lead to hepatic failure even requiring a transplant to prevent severe internal injuries or even death, is not early and correctly treated. PMID:24152394

  15. Melanie Klein in Buenos Aires: beginnings and developments.

    PubMed

    Etchegoyen, R Horacio; Zysman, Samuel

    2005-06-01

    In the first decades of the 20th century, Freud was known and quoted in Latin America by an elite of enlightened minds. In the 1940s a convergence took place in Buenos Aires of European exiles with local pioneers, and thus the Argentine Psychoanalytical Association was founded in 1942. Since then psychoanalysis has grown steadily and has spread into hospitals and universities, influencing culture at large. The socioeconomic situation of that time permitted this phenomenon to develop, to the astonishment of observers. In this paper the authors study the strong influence of Kleinian thought during the first 30 years of this development. The original works of local thinkers constitute the intellectual capital that sustains the idea of an 'Argentine psychoanalytic school'. During the 1970s, both society and psychoanalysis endured deep and complex changes. Lacan's teachings gained support and Klein's influence began to decline. At present the Buenos Aires Kleinians keep working, while their relationship with Lacanians and other schools is calmer. Respectful discussions became thus possible, oriented to strictly scientific differences. PMID:16096079

  16. [RESEARCH AMONG PSYCHIATRISTS IN TRAINING IN CIUDAD DE BUENOS AIRES].

    PubMed

    Peskin, Viviana A; Berrío Cuartas, Diana M; Villafañe, Claudia I; Pereyra, Walter D; Martínez Didolich, Laura C; Cesoni, Oscar M; Uriarte, Martín A

    2015-01-01

    Research is a cornerstone in the training of medical doctors in any specialty, whereas it substantially contributes to good clinical practice. The aim of this study is to determine the importance of research among psychiatrists in training in Ciudad de Buenos Aires. A cross sectional descriptive study using a paper survey, which was voluntary and anonymous, was sent to psychiatrists in training in different training centers between October-November 2013. 76.6% considered research training deficient; 27.8% participated in a research project during their psychiatric training and only 21.5% presented their results at a scientific activity. 95.6% participants considered important to include research in their training. In summary, a small proportion of psychiatrists in training who participated in this study conducted research and we observed limited experience in the area, as opposed to the interest in being trained in this field.

  17. Molecular characterization of Ehrlichia canis infecting dogs, Buenos Aires.

    PubMed

    Cicuttin, Gabriel L; De Salvo, María N; Gury Dohmen, Federico E

    2016-07-01

    Canine monocytic ehrlichiosis (CME) is a worldwide potentially fatal tick-borne rickettsial disease of dogs caused by Ehrlichia canis and transmitted by Rhipicephalus sanguineus sensu lato. CME diagnosis includes indirect (serology) and direct (e.g. blood smears and PCR) methods. PCR is more sensitive and specific than direct microscopic examination and positive PCR results confirm infection, whereas positive serologic test results only confirm exposure. The aim of the present study was to perform a molecular characterization of E. canis from canine samples of the Metropolitan Area of Buenos Aires. We studied 223 blood samples of dogs submitted to our institute for CME diagnoses. The samples were initially screened for Anaplasmataceae family by PCR, resulting in 30 positive dogs (13.4%). Subsequently, positive DNAs were analyzed by nested PCR 16S rRNA specific for E. canis or Anaplasma platys, resulting in 15 (6.7%) and 16 (7.2%) positive dogs, respectively. For molecular characterization, samples positive for E. canis were subjected to amplification of a fragment of the dsb and p28 genes. The nucleotide sequences obtained for the dsb fragment resulted in 100% identity with others E. canis found in dogs from different regions of worldwide. The nucleotide sequences obtained for p28 gene resulted in 100% of identity with each other and closely with E. canis str. Jaboticabal (Brazil). Identity with others sequences of E. canis ranged from 76.9 to 79.7%. The occurrence of canine cases molecularly confirmed in Metropolitan Area of Buenos Aires highlights the need for more studies in order to understand epidemiological factors associated with CME, especially the disease transmission dynamic in South America given the existence of two lineages of R. sanguineus sensu lato with different vectorial capacity for transmission of E. canis. PMID:27236582

  18. Tobacco use among high school students in Buenos Aires, Argentina.

    PubMed Central

    Morello, P; Duggan, A; Adger, H; Anthony, J C; Joffe, A

    2001-01-01

    OBJECTIVES: This study assessed the prevalence and correlates of tobacco use among high school students in Buenos Aires, Argentina. METHODS: Anonymous, self-administered questionnaires were given to 3909 8th and 11th graders in a stratified random sample of 49 public and private schools. The instrument included items from American surveys, translated and validated among Argentinean teens. Multiple logistic regression analysis was used to estimate possible effects on smoking behavior of environment, students' personal characteristics, and their knowledge, beliefs, and attitudes regarding smoking. RESULTS: Of 8th and 11th graders, 20% and 43%, respectively, were classified as current smokers. Overall, 29% of males and 32% of females were current smokers. Students attending public schools were more likely to smoke than those in private schools (P < .05). Current smoking was associated with having a best friend who smokes, reporting that more than 50% of friends of the same sex smoke, having positive attitudes and beliefs toward smoking, and having a positive intention to smoke within the next year (all P < .001). CONCLUSIONS: Over 20% of the 8th graders in our sample were current smokers; prevention efforts must therefore start early. PMID:11213790

  19. Bartonella spp. in cats from Buenos Aires, Argentina.

    PubMed

    Cicuttin, Gabriel L; Brambati, Diego F; De Gennaro, María F; Carmona, Fernando; Isturiz, María L; Pujol, Laura E; Belerenian, Guillermo C; Gil, Horacio

    2014-01-10

    In Argentina, data on the presence of members of the genus Bartonella is scarce. To increase knowledge about these zoonotic pathogens in this country, the presence and variability of Bartonella spp. was investigated in cats and dogs from Buenos Aires. Bartonella spp. was detected in 17.8% of cats, while all dogs tested negative by PCR and Reverse Line Blot. B. henselae was the most frequent species, being detected in 11.9% (14/101), while B. clarridgeiae was found in only 5.9% (6/101) of the cats. Afterwards, B. henselae isolates and positive blood samples were characterized by Multiple Locus Sequence Typing (MLST) and Multiple Locus Variable Number Tandem Repeats Analysis (MLVA). As result, four different MLST sequence types (ST) and eight MLVA profiles were identified. ST 1 was the most frequent variant found in cats, followed by ST 8. Interestingly, some of the MLVA profiles that were detected in this study have been previously associated with human disease, and represents a potential risk of infection. Veterinarians and physicians should consider the presence of these emerging pathogens in their diagnostic routine.

  20. Spatial analysis of the tuberculosis treatment dropout, Buenos Aires, Argentina.

    PubMed

    Herrero, María Belén; Arrossi, Silvina; Ramos, Silvina; Braga, Jose Ueleres

    2015-01-01

    OBJECTIVE Identify spatial distribution patterns of the proportion of nonadherence to tuberculosis treatment and its associated factors. METHODS We conducted an ecological study based on secondary and primary data from municipalities of the metropolitan area of Buenos Aires, Argentina. An exploratory analysis of the characteristics of the area and the distributions of the cases included in the sample (proportion of nonadherence) was also carried out along with a multifactor analysis by linear regression. The variables related to the characteristics of the population, residences and families were analyzed. RESULTS Areas with higher proportion of the population without social security benefits (p = 0.007) and of households with unsatisfied basic needs had a higher risk of nonadherence (p = 0.032). In addition, the proportion of nonadherence was higher in areas with the highest proportion of households with no public transportation within 300 meters (p = 0.070). CONCLUSIONS We found a risk area for the nonadherence to treatment characterized by a population living in poverty, with precarious jobs and difficult access to public transportation. PMID:26270011

  1. Temporal variability of the Buenos Aires, Argentina, urban heat island

    NASA Astrophysics Data System (ADS)

    Camilloni, Inés; Barrucand, Mariana

    2012-01-01

    This paper describes the statistical characteristics and temporal variability of the urban heat island (UHI) intensity in Buenos Aires using 32-year surface meteorological data with 1-h time intervals. Seasonal analyses show that the UHI intensity is strongest during summer months and an "inverse" effect is found frequently during the afternoon hours of the same season. During winter, the UHI effect is in the minimal. The interannual trend and the seasonal variation of the UHI for the main synoptic hours for a longer record of 48 years are studied and associated to changes in meteorological factors as low-level circulation and cloud amount. Despite the population growth, it was found a negative trend in the nocturnal UHI intensity that could be explained by a decline of near clear-sky conditions, a negative trend in the calm frequencies and an increase in wind speed. Urban to rural temperature differences and rural temperatures are negatively correlated for diurnal and nocturnal hours both for annual and seasonal scales. This result is due to the lower interannual variability of urban temperatures in comparison to rural ones.

  2. Spatial analysis of the tuberculosis treatment dropout, Buenos Aires, Argentina

    PubMed Central

    Herrero, María Belén; Arrossi, Silvina; Ramos, Silvina; Braga, Jose Ueleres

    2015-01-01

    OBJECTIVE Identify spatial distribution patterns of the proportion of nonadherence to tuberculosis treatment and its associated factors. METHODS We conducted an ecological study based on secondary and primary data from municipalities of the metropolitan area of Buenos Aires, Argentina. An exploratory analysis of the characteristics of the area and the distributions of the cases included in the sample (proportion of nonadherence) was also carried out along with a multifactor analysis by linear regression. The variables related to the characteristics of the population, residences and families were analyzed. RESULTS Areas with higher proportion of the population without social security benefits (p = 0.007) and of households with unsatisfied basic needs had a higher risk of nonadherence (p = 0.032). In addition, the proportion of nonadherence was higher in areas with the highest proportion of households with no public transportation within 300 meters (p = 0.070). CONCLUSIONS We found a risk area for the nonadherence to treatment characterized by a population living in poverty, with precarious jobs and difficult access to public transportation. PMID:26270011

  3. Spatial analysis of the tuberculosis treatment dropout, Buenos Aires, Argentina.

    PubMed

    Herrero, María Belén; Arrossi, Silvina; Ramos, Silvina; Braga, Jose Ueleres

    2015-01-01

    OBJECTIVE Identify spatial distribution patterns of the proportion of nonadherence to tuberculosis treatment and its associated factors. METHODS We conducted an ecological study based on secondary and primary data from municipalities of the metropolitan area of Buenos Aires, Argentina. An exploratory analysis of the characteristics of the area and the distributions of the cases included in the sample (proportion of nonadherence) was also carried out along with a multifactor analysis by linear regression. The variables related to the characteristics of the population, residences and families were analyzed. RESULTS Areas with higher proportion of the population without social security benefits (p = 0.007) and of households with unsatisfied basic needs had a higher risk of nonadherence (p = 0.032). In addition, the proportion of nonadherence was higher in areas with the highest proportion of households with no public transportation within 300 meters (p = 0.070). CONCLUSIONS We found a risk area for the nonadherence to treatment characterized by a population living in poverty, with precarious jobs and difficult access to public transportation.

  4. John Tweedie and Charles Darwin in Buenos Aires.

    PubMed

    Ollerton, Jeff; Chancellor, Gordon; van Wyhe, John

    2012-06-20

    The journey of exploration undertaken by Charles Darwin FRS during the voyage of HMS Beagle has a central place within the historical development of evolutionary theory and has been intensively studied. Despite this, new facts continue to emerge about some of the details of Darwin's activities. Drawing on recently published Darwin material and unpublished letters in the archives of the Royal Botanic Gardens, Kew, we document a hitherto unexamined link between Darwin and John Tweedie (1775-1862), a relatively obscure Scottish gardener turned South American plant collector. All of the available evidence points to a meeting between the two men in Buenos Aires in 1832. Tweedie provided Darwin with information about the geography of the Rio Paraná, including the locality of fossilized wood eroding from the river bank. It also seems likely that Tweedie supplied Darwin with seeds that he later shipped back to John Stevens Henslow in Cambridge. Although this brief meeting was at the time relatively unimportant to either man, echoes of that encounter have resonated with Tweedie's descendants to the present day and have formed the basis for a family story about a written correspondence between Darwin and Tweedie. Local information supplied to Darwin by residents such as Tweedie was clearly important and deserves further attention.

  5. Sociophonetic Variation in the Production and Perception of Obstruent Voicing in Buenos Aires Spanish

    ERIC Educational Resources Information Center

    Rohena-Madrazo, Marcos

    2011-01-01

    This dissertation presents an instrumental study of variation in fricative voicing in Buenos Aires Spanish (BAS), particularly with respect to the devoicing change of the postalveolar fricative: /y/greater than/[function of]/. It proposes a novel way of determining the completion of this change by comparing the percentage voicing of the…

  6. Immigration and Educational Inclusion: Preliminary Evidence from Three Schools in Buenos Aires, Argentina

    ERIC Educational Resources Information Center

    Astiz, M. Fernanda

    2015-01-01

    This exploratory comparative case study examines three schools in the Autonomous City of Buenos Aires that have a considerable number of recent immigrant students. The article illustrates how these schools advance ideas of inclusiveness and pluralism through the curriculum and educational opportunities, as mandated by the national education law of…

  7. The Public Service of the Official Time at the Observatorio Naval Buenos Aires

    NASA Astrophysics Data System (ADS)

    Cifuentes, A.; Esperón, C.; Osorio, J.; Amenna, J.; Salday, J.; Silva, G.

    2014-06-01

    The Observatorio Naval Buenos Aires (ONBA), through the Public Service of the Official Time Argentina (hereinafter, Time Service) generates, maintains and disseminates UTC (ONBA), which is the basis of the Argentine legal time. We present activities, projects such as time stamping to digital signature and new equipment acquired to improve traceability UTC (ONBA) to UTC.

  8. The Library Science Institute of the University of Buenos Aires: Summary of Its Objectives and Services.

    ERIC Educational Resources Information Center

    Gravenhorst, Hans

    The main objectives of the Institute, which was founded in 1941, are to act as a center for bibliographic and documentary information and to be the co-ordinating body for the libraries belonging to the ten schools of the University of Buenos Aires. To fulfill this objective, the Institute library possesses the most important sources of…

  9. Earnings and Education in Argentina: An Analysis of the 1985 Buenos Aires Household Survey.

    ERIC Educational Resources Information Center

    Kugler, Bernardo; Psacharopoulos, George

    1989-01-01

    Using data from the 1985 Buenos Aires (Argentina) Household Survey of 4,500 employed individuals, earnings differences are explained by a set of individual human capital characteristics. Returns to education investments are then estimated for different levels and types of schooling. Secondary and higher education investments need to be retargeted.…

  10. Reform Process in the University of Buenos Aires: Perspective of the Professional Fields of Engineering.

    ERIC Educational Resources Information Center

    de Camilloni, Alicia W.; Fernandez, Marcela; Zadunaisky, Diana

    The University of Buenos Aires (UBA) is committed to a reform process which involves, among others, the curricular axis. The work described in this paper is part of the activities specially concerned with the studies of engineering. Seventy-five professionals were invited to discuss new possible patterns for the perspective of the professional…

  11. Cryptosporidium parvum GP60 subtypes in dairy cattle from Buenos Aires, Argentina

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cryptosporidium parvum from 73 dairy calves less than two months old from Buenos Aires province (Argentina) were molecularly characterized using sequence analysis of the GP60 gene. Seventy five sequences were obtained, and seven different subtypes were identified, all belonging to the IIa subtype f...

  12. [Winter serum levels of 25-hydroxy-vitamin D in Ushuaia and Buenos Aires].

    PubMed

    Oliveri, M B; Ladizesky, M; Somoza, J; Martínez, L; Mautalen, C

    1990-01-01

    Public Health Annals recording diagnosis of nutritional rickets in patients admitted in Public Hospitals disclosed that from birth to age 14, in the period 1980-1981, the incidence was 2.7 higher in the Patagonia (latitude 39 degrees S to 55 degrees S) compared with the Pampeana Region and 8.5 higher than in the rest of the country. After informed parental consent 37 healthy children of Buenos Aires (34 degrees S) with an age of (Av +/- 1 SD) 7.0 +/- 1.2 years, 29 with an age of 13.1 +/- 1.5 years and 63 of Ushuaia (55 degrees S) with an age of 7.1 +/- 0.8 years were studied at the end of winter (August). Serum levels of 25-OH-D were as follows (mean +/- SE): Buenos Aires: 21.1 +/- 2.03 ng/ml (Average: seven years old), 19.0 +/- 1.18 ng/ml (children of thirteen years old) and Ushuaia: 9.3 +/- 0.64 ng/ml (p less than 0.001) (Fig. 2). Serum levels were below 8 ng/ml in 52% of the children in Ushuaia but only in 9% in Buenos Aires. Serum calcium and alkaline phosphatase levels were similar in the two groups but serum phosphate was higher in Ushuaia (Table 1). The calcium intake was greater in Ushuaia (811 +/- 49 mg/day) than in Buenos Aires (634 +/- 61 mg/day) and was correlated with 25-OH-D levels in children of Ushuaia (r = 0.50, p less than 0.001) but not in Buenos Aires (r = 0.08). The main source of calcium intake was vitamin D fortified milk. These results disclosed a significantly diminished level of serum 25-OH-D in Ushuaia.(ABSTRACT TRUNCATED AT 250 WORDS)

  13. Characterization of aerosol particles from Buenos Aires City and its subway system: PIXE and SEM/EDX

    SciTech Connect

    Murruni, L. G.; Debray, M. E.; Minsky, D.; Kreiner, A. J.; Burlon, A.; Davidson, M.; Davidson, J.; Ozafran, M.; Vazquez, M. E.; Rosenbusch, M.; Ulke, A. G.; Solanes, V.

    2007-02-12

    This study analyzes total suspended particle (TSP) samples collected at two sites of Buenos Aires City (34S, 58W). One site (San Martin) placed 17 km from city center, and the other one at an underground subway station (Diagonal Norte) in downtown Buenos Aires. In both cases, gravimetric analysis has been performed, while elemental analysis using PIXE has been only carried out in the first case. To the best our knowledge, this is the first airborne particle measurement perform at a Buenos Aires underground subway station.

  14. [Dengue imported from Paraguay to Buenos Aires. Clinical and epidemiological report of 38 cases].

    PubMed

    Seijo, A; Cernigoi, B; Deodato, B

    2001-01-01

    Thirty eight patients with classic dengue fever, were studied from December 1999 up to April 2000. All of them acquired the infection in Paraguay; 66% of patients arrived at Buenos Aires metropolitan area within the viremia period. Given Aedes aegypti abundance in the region they represent a high risk for local transmission. Unusual clinical findings in these patients were diarrhea (29%), transitory rise of seric aspartate aminotransferase (52%) and pruritic rash in all cases. Only 15.7% showed a biphasic course and none of the five patients with hemorrhages had a positive loop test. These observations show that dengue fever could be misdiagnosed as gastroenteritis or flue-like illness. These patients remain at risk of suffering hemorrhagic dengue. DEN 1 has not been reported in Buenos Aires. This fact and the unusual number of imported cases, represent a serious public health problem.

  15. [Serological evidence of St. Louis encephalitis virus circulation in birds from Buenos Aires City, Argentina].

    PubMed

    Beltrán, Fernando J; Díaz, Luis A; Konigheim, Brenda; Molina, José; Beaudoin, Juan B; Contigiani, Marta; Spinsanti, Lorena I

    2015-01-01

    Our goal was to determine the presence of neutralizing antibodies against St. Louis encephalitis virus (SLEV) and West Nile virus (WNV) in sera of wild and domestic birds from Buenos Aires City, Argentina. From October 2012 to April 2013, 180 samples were collected and processed by the microneutralization technique. A 7.2% of the sampled birds were seropositive for SLEV, while no seropositive birds for WNV were detected.

  16. Serological evidence of Rickettsia and Coxiella burnetii in humans of Buenos Aires, Argentina.

    PubMed

    Cicuttin, Gabriel Leonardo; Degiuseppe, Juan Ignacio; Mamianetti, Andrea; Corin, Marcela Viviana; Linares, María Cielo; De Salvo, María Nazarena; Dohmen, Federico Eugenio Gury

    2015-12-01

    In Buenos Aires city (Argentina), the circulation of these agents has been detected mainly in vectors and animals, few human cases having been described. The aim of our study was to determine the seroprevalence of Rickettsia (spotted fever--SFG--and typhus--TG--groups) and Coxiella burnetii (Q fever agent) in residents of Buenos Aires city. The study involved 99 participants. Rickettsia IgG antibodies against SFG and TG were detected by IFA in 28.3% and 16.2% of serum samples, respectively. SFG titers were mostly 1/64 (53.6%) with a maximum of 1/512 (3.5%) whereas TG titers ranged between 1/64 (62.5%) and 1/256 (6.3%). Only one sample showed a titer of 1/32 for C. burnetii (phases I and II). The circulation of these pathogens in urban areas such as the city of Buenos Aires should be considered by health services, especially at the primary care level. PMID:26616661

  17. Serological evidence of Rickettsia and Coxiella burnetii in humans of Buenos Aires, Argentina.

    PubMed

    Cicuttin, Gabriel Leonardo; Degiuseppe, Juan Ignacio; Mamianetti, Andrea; Corin, Marcela Viviana; Linares, María Cielo; De Salvo, María Nazarena; Dohmen, Federico Eugenio Gury

    2015-12-01

    In Buenos Aires city (Argentina), the circulation of these agents has been detected mainly in vectors and animals, few human cases having been described. The aim of our study was to determine the seroprevalence of Rickettsia (spotted fever--SFG--and typhus--TG--groups) and Coxiella burnetii (Q fever agent) in residents of Buenos Aires city. The study involved 99 participants. Rickettsia IgG antibodies against SFG and TG were detected by IFA in 28.3% and 16.2% of serum samples, respectively. SFG titers were mostly 1/64 (53.6%) with a maximum of 1/512 (3.5%) whereas TG titers ranged between 1/64 (62.5%) and 1/256 (6.3%). Only one sample showed a titer of 1/32 for C. burnetii (phases I and II). The circulation of these pathogens in urban areas such as the city of Buenos Aires should be considered by health services, especially at the primary care level.

  18. Complete genome sequencing of dengue virus type 1 isolated in Buenos Aires, Argentina.

    PubMed

    Barrero, Paola R; Mistchenko, Alicia S

    2004-05-01

    Dengue (DEN) constitutes a major viral arthropod-borne human illness. South America was last considered free of dengue two decades ago when a dramatic increase in the number of dengue fever and hemorrhagic dengue cases had been reported. Five viruses were isolated in Buenos Aires City from the 1999-2000 Paraguay outbreak. RT-PCRs obtained directly from plasma were cloned into pGemT vectors and sequences of the structural genes and NS1 were analyzed. Three viruses were full-length sequenced from RT-PCR obtained from cell-culture isolates. Excess of synonymous over non-synonymous mutations suggested that the structural proteins were under strong functional constraints while a weak purifying selection was operating in the whole polyprotein. Sequence diversity and selective pressures varied among patients but results were significantly above the procedure threshold. One sample showed small-plaque phenotype and impaired growth coupled to 3'untranslated region mutations. Phylogenetic analysis of full-length sequences split Buenos Aires isolates into two clusters within American DEN-1 genotype V: Clade I was phylogenetically linked to Brazilian samples and Clade II with samples from Paraguay and Northeastern Argentina. In Buenos Aires City, only dengue virus serotype 1 imported from Paraguay has been detected, though without evidence of local transmission.

  19. [Phenotypic and genotypic characterization of imipenem-resistant Pseudomonas aeruginosa isolated in a Buenos Aires hospital].

    PubMed

    Cejas, D; Almuzara, M; Santella, G; Tuduri, A; Palombarani, S; Figueroa, S; Gutkind, G; Radice, M

    2008-01-01

    From 129 P. aeruginosa isolated at a health care centre located in Buenos Aires (Hospital "Eva Perón"), 14% produced IMP-13. Although 18 isolates were metallo-beta-lactamases (MBL) producers, only those isolates that displayed altered outer membrane protein profiles correlated with the resistant category according to CLSI or even Subcomisión de Antimicrobianos, SADEBAC, AAM. Phenotypic screening of metallo-beta-lactamases proved to be appropriate for detecting MBL producing isolates. IMP-13 producing isolates corresponded to at least five different clonal types, which not only suggests the dissemination of the resistant strain but also of the resistant marker.

  20. Atmospheric Boundary Layer Height Evolution with Lidar in Buenos Aires from 2008 to 2011

    NASA Astrophysics Data System (ADS)

    Pawelko, Ezequiel Eduardo; Salvador, Jacobo Omar; Ristori, Pablo Roberto; Pallotta, Juan Vicente; Otero, Lidia Ana; Quel, Eduardo Jaime

    2016-06-01

    The analysis of the atmospheric boundary layer top height evolution is obtained from 2008 to 2011 in Buenos Aires using the multiwavelength lidar located at CEILAP (CITEDEF-CONICET) (34°33' S; 58°30' W; 17 m asl). Algorithms recognition based on covariance wavelet transform are applied to obtain seasonal statistics. This method is being evaluated for use in the Lidar Network in Argentina and it is being deployed in Patagonia region currently. The technique operates in real time in both low and high aerosol loads and with almost no human supervision.

  1. Poor, ill, and sometimes abandoned: tubercular children in Buenos Aires, 1880-1920.

    PubMed

    Reber, Vera Blinn

    2002-04-01

    Ill children with chronic diseases, such as tuberculosis, have faced difficult lives. Poverty proved a factor in their susceptibility to disease, their abandonment, and their treatment. When public health policies in Buenos Aires shifted from ignoring children to viewing them as victims who needed protection, government agencies, charitable organizations, public schools, and hospitals developed special programs that emphasized both prevention and cure of childhood tuberculosis. Argentine physicians and hygienists supported programs that were similar to those in Europe and the United States. Despite efforts, from 1880 to 1920, diagnosis of tuberculosis remained problematic, health professionals failed to prevent tuberculosis in children, and physicians were unable to cure the disease.

  2. Urban political ecologies of informal recyclers׳ health in Buenos Aires, Argentina.

    PubMed

    Parizeau, Kate

    2015-05-01

    Buenos Aires׳ informal recyclers (cartoneros) confront multiple health hazards in their work. Based in a survey with (n=397) informal recyclers, this study establishes that these workers experience uneven health landscapes as evidenced through their health outcomes, the social determinants of their health, and their living and working environments. I argue that the analytical framework of urban political ecology can provide insights to the ways that the urban environments where cartoneros live and work are socially-constructed phenomena, drawing on concepts of crisis, metabolism, and multi-scalar analyses. PMID:25770437

  3. [Buenos Aires, between Eros and Thanatos. Prostitution as dysgenic threat (1930-1955)].

    PubMed

    Miranda, Marisa A

    2012-01-01

    This paper explores the medical-legal discourse around female prostitution that was articulated in Buenos Aires during the first decades of the 20th century. It focuses on the use of different ideological resources and on the policies and laws that were introduced to gain control over the sex trade and describes parallelisms between prostitution, disease and crime. In fact, the capital of Argentina was internationally known as a centre of prostitution and white slave trafficking, and Jewish participation in these activities allowed the consolidation of xenophobic theories. Medicine and law, among other disciplines, made important inputs into a simplified approach to this problem. PMID:22849217

  4. New Dalyelliidae (Platyhelminthes, Rhabditophora) from Buenos Aires Province, Argentina, and their stylet ultrastructure.

    PubMed

    Damborenea, Cristina; Brusa, Francisco; Noreña, Carolina

    2007-08-01

    Two new species of Dalyellidae, Dalyellia callvucurai n. sp. and Gieysztoria namuncurai n. sp., are described from temporary freshwater environments in central Buenos Aires Province, Argentina. The ultrastructure of the stylets of both species is also described. The new species of the genus Dalyellia is the second species of the genus found in the neotropics and the first whose stylet has been studied with scanning electron microscopy. Gieysztoria namuncurai n. sp. joins the seven species of the genus known in Argentina. The ultrastructural characteristics of the stylet place it in the Inaequales group, with complex stylets.

  5. Urban political ecologies of informal recyclers׳ health in Buenos Aires, Argentina.

    PubMed

    Parizeau, Kate

    2015-05-01

    Buenos Aires׳ informal recyclers (cartoneros) confront multiple health hazards in their work. Based in a survey with (n=397) informal recyclers, this study establishes that these workers experience uneven health landscapes as evidenced through their health outcomes, the social determinants of their health, and their living and working environments. I argue that the analytical framework of urban political ecology can provide insights to the ways that the urban environments where cartoneros live and work are socially-constructed phenomena, drawing on concepts of crisis, metabolism, and multi-scalar analyses.

  6. [Buenos Aires, between Eros and Thanatos. Prostitution as dysgenic threat (1930-1955)].

    PubMed

    Miranda, Marisa A

    2012-01-01

    This paper explores the medical-legal discourse around female prostitution that was articulated in Buenos Aires during the first decades of the 20th century. It focuses on the use of different ideological resources and on the policies and laws that were introduced to gain control over the sex trade and describes parallelisms between prostitution, disease and crime. In fact, the capital of Argentina was internationally known as a centre of prostitution and white slave trafficking, and Jewish participation in these activities allowed the consolidation of xenophobic theories. Medicine and law, among other disciplines, made important inputs into a simplified approach to this problem.

  7. Ergonomic analysis for elementary school teachers. Preliminary trial for elementary schools in the province of Buenos Aires.

    PubMed

    Cuenca, Gabriela; Nusshold, Patricio

    2012-01-01

    This article presents the results of a preliminary trial, in the context of an intervention, developed by a team of ergonomists under the request of the Occupational Health Department of the General Management of Schools in the Province of Buenos Aires, Argentina. We present a reformulation of the request and the results obtained in 2009 from observations and interviews held in three schools in the province of Buenos Aires in which different jobs positions were assessed. From this stage onwards, the tools were validated and new aspects were shown, particularly in relation to the high variety of real situations in the education sector.

  8. A method for optimizing waste collection using mathematical programming: a Buenos Aires case study.

    PubMed

    Bonomo, Flavio; Durán, Guillermo; Larumbe, Frederico; Marenco, Javier

    2012-03-01

    A method is proposed that uses operations research techniques to optimize the routes of waste collection vehicles servicing dumpster or skip-type containers. The waste collection problem is reduced to the classic travelling salesman problem, which is then solved using the Concorde solver program. A case study applying the method to the collection system in the southern zone of Buenos Aires is also presented. In addition to the typical minimum distance criterion, the optimization problem incorporates the objective of reducing vehicle wear and tear as measured by the physics concept of mechanical work. The solution approach, employing graph theory and mathematical programming tools, is fully described and the data correction process is also discussed. The application of the proposed method minimized the distance travelled by each collection vehicle in the areas studied, with actual reductions ranging from 10 to 40% of the existing routes. The shortened distances led in turn to substantial decreases in work done and therefore in vehicle wear and tear. Extrapolation of the results to the entire southern zone of Buenos Aires indicates potential savings for the civic authorities of more than US $200,000 per year in addition to the qualitative impacts of less traffic disruption, less vehicle driver fatigue and less pollution.

  9. The smoke episode in Buenos Aires, 15-20 April 2008

    NASA Astrophysics Data System (ADS)

    Berbery, Ernesto Hugo; Ciappesoni, Hector C.; Kalnay, Eugenia

    2008-11-01

    The smoke that affected the city of Buenos Aires and its suburbs (approximate population, 13M) in mid April 2008 was an extreme event without historical precedent. The episode resulted in an increase of health problems among the population (respiratory problems, eye irritation) and, due to poor visibility, led to hazardous driving conditions and accidents that forced the intermittent closure of major highways. The origin of the smoke was traced to pasture burning in the La Plata River delta, to the northwest of Buenos Aires. Unfortunately, the increased shifting of livestock to the La Plata River delta may result in more common smoke episodes due to associated biomass burning practices. We clarify the mechanisms that resulted in this extreme episode, including the contribution of the La Plata River local circulations to the intensity of the event. We further show its high predictability using high resolution regional model simulations and forecasts. Our results suggest that a high resolution regional model could be used to monitor and predict several days in advance the atmospheric transport of smoke. These results could have policy implications, as preventive measures on biomass burning could be put in effect when smoke from the fires is predicted to affect a densely populated area.

  10. A method for optimizing waste collection using mathematical programming: a Buenos Aires case study.

    PubMed

    Bonomo, Flavio; Durán, Guillermo; Larumbe, Frederico; Marenco, Javier

    2012-03-01

    A method is proposed that uses operations research techniques to optimize the routes of waste collection vehicles servicing dumpster or skip-type containers. The waste collection problem is reduced to the classic travelling salesman problem, which is then solved using the Concorde solver program. A case study applying the method to the collection system in the southern zone of Buenos Aires is also presented. In addition to the typical minimum distance criterion, the optimization problem incorporates the objective of reducing vehicle wear and tear as measured by the physics concept of mechanical work. The solution approach, employing graph theory and mathematical programming tools, is fully described and the data correction process is also discussed. The application of the proposed method minimized the distance travelled by each collection vehicle in the areas studied, with actual reductions ranging from 10 to 40% of the existing routes. The shortened distances led in turn to substantial decreases in work done and therefore in vehicle wear and tear. Extrapolation of the results to the entire southern zone of Buenos Aires indicates potential savings for the civic authorities of more than US $200,000 per year in addition to the qualitative impacts of less traffic disruption, less vehicle driver fatigue and less pollution. PMID:21460074

  11. Megacity and country emissions from combustion sources-Buenos Aires-Argentina

    NASA Astrophysics Data System (ADS)

    Dawidowski, L.; Gomez, D.; Matranga, M.; D'Angiola, A.; Oreggioni, G.

    2010-12-01

    Historic time series (1970-2006) emissions of greenhouse gases and air pollutants arising from stationary and mobile combustion sources were estimated at national level for Argentina and at regional level for the metropolitan area of Buenos Aires (MABA). All emissions were estimated using a bottom-up approach following the IPCC good practice guidance. For mobile sources, national emissions include all transport categories. Regional emissions account thus far only for on-road. For national emissions, methodologies and guidance by the IPCC were employed, applying the highest possible tier and using: i)country-specific emission factors for carbon and sulphur and technology-based information for other species, ii)activity data from energy balance series (1970-2007), and iii)complementary information concerning the non-energy use of fuels. Regional emissions in 2006 were estimated in-depth using a technology-based approach for the city of Buenos Aires (CBA) and the 24 neighboring districts composing the MABA. A regional emissions factors database was developed to better characterize Latin American fleets and driving conditions employing COPERT III-IV algorithms and emission factors measured in dynamometers and circulating vehicles in Argentina, Brazil, Chile and Colombia. Past emissions were back estimated from 2005 to 1970 using the best available information, which differs greatly among categories, spatial disaggregation and time periods. The time series of stationary and mobile combustion sources at the national and regional level allowed the identification of distinct patterns. National greenhouse gas emissions in 2006 amounted to ~ 150 million ton CO2-equivalent, 70% of which were contributed by stationary sources. On-road transport was the major contributor within mobile sources (28.1 %). The increasing emissions trends are dominated by on-road transport, agriculture and residential categories while the variability is largely associated with energy industries

  12. Motivation and Attitudes of Students of English as a Foreign Language at ICANA in Buenos Aires, Argentina.

    ERIC Educational Resources Information Center

    Stein, Lia Diana Kamhi

    Both the motivation for and attitudes of adult Argentine students studying English as a foreign language (EFL) are investigated. A questionnaire was administered to 114 adult students attending EFL classes at beginning, intermediate, and advanced levels at the Instituto Cultural Argentino Norteamericano (ICANA) in Buenos Aires. The findings…

  13. Methodological and Ethical Dilemmas Encountered during Field Research of Family Violence Experienced by Adolescent Women in Buenos Aires

    ERIC Educational Resources Information Center

    Luxardo, Natalia; Colombo, Graciela; Iglesias, Gabriela

    2011-01-01

    The purpose of this article is to examine some obstacles and dilemmas related to methodological strategies and ethical considerations that arose during the fieldwork of research focused on family violence during the stages of pregnancy and childbirth in adolescent females in Buenos Aires during 2007. From this study, we are able to contribute some…

  14. "Zafar," So Good: Middle-Class Students, School Habitus and Secondary Schooling in the City of Buenos Aires (Argentina)

    ERIC Educational Resources Information Center

    Meo, Analia Ines

    2011-01-01

    This article examines how students from the "loser" sections of the middle class dealt with the game of secondary schooling in a "good" state school in the city of Buenos Aires (Argentina). It engages with Bourdieu's theory of social practice and, in particular, with its concepts of game, habitus and cultural capital. It argues that middle-class…

  15. Decentralization and Structural Change in Secondary Education in Argentina: The Case of the Province of Buenos Aires

    ERIC Educational Resources Information Center

    Acedo, Clementina; Gorostiaga, Jorge M.; Senen-Gonzalez, Silvia

    2007-01-01

    Over the past decade, Argentina created and implemented a compulsory lower-secondary education level, within an ambitious educational reform programme. This article addresses the reform at the national level, diverse provincial responses, and the particular way that the powerful province of Buenos Aires appropriated the structural change. The…

  16. Functional foods and nutraceuticals in a market of bolivian immigrants in Buenos Aires (Argentina).

    PubMed

    Pochettino, María Lelia; Puentes, Jeremías P; Buet Costantino, Fernando; Arenas, Patricia M; Ulibarri, Emilio A; Hurrell, Julio A

    2012-01-01

    This paper presents the results of a research in urban ethnobotany, conducted in a market of Bolivian immigrants in the neighborhood of Liniers, Ciudad Autónoma de Buenos Aires (Argentina). Functional foods and nutraceuticals belonging to 50 species of 18 families, its products, and uses were recorded. Some products are exclusive from the Bolivian community; others are frequent within the community, but they are also available in the general commercial circuit; they are introduced into it, generally, through shops called dietéticas ("health-food stores"), where products associated with the maintenance of health are sold. On this basis, the traditional and nontraditional components of the urban botanical knowledge were evaluated as well as its dynamics in relation to the diffusion of the products. Both the framework and methodological design are innovative for the studies of the urban botanical knowledge and the traditional markets in metropolitan areas.

  17. [Decentralization and equity: public health expenditure in the municipalities of the Province of Buenos Aires].

    PubMed

    Lago, Fernando Pablo; Moscoso, Nebel Silvana; Elorza, María Eugenia; Ripari, Nadia Vanina

    2012-12-01

    In this paper we analyze the degree of equity in access to the public health care system in the Province of Buenos Aires (Argentina). Through a quantitative retrospective study, we analyze the inequalities in the distribution of the total public health expenditure per capita. This variable is used as a proxy for the ability of the inhabitants of each jurisdiction to access health care services. The results indicate the existence of large disparities in the levels of expenditure devoted to the population without health coverage. Moreover, the existence of greater health care needs (estimated using infant mortality rates and percentage of homes with basic needs unmet) does not translate into higher levels of public expenditure. Finally, we detect a positive association between the relative wealth of municipalities (measured by the gross geographic product per capita) and the public health expenditure per capita. PMID:23681459

  18. Population data for DXS6800, DXS101 and DXS8377 loci from Buenos Aires (Argentina).

    PubMed

    Noseda, Pablo A; Kenline, Jaclyn; Manning, Samantha; Gangitano, David A

    2013-07-01

    The X-chromosomal short tandem repeats (X-STRs) DXS6800, DXS101 and DXS8377 were analysed in a population sample from Buenos Aires (Argentina) using a polymerase chain reaction (PCR) multiplex approach with fluorescent detection. We present allele frequencies for these loci in a population comprising 113 women and 99 men. The Hardy-Weinberg equilibrium (HWE) was tested in the female sample and no significant deviations were observed. The homogeneity of allele frequencies of men and women was compared by the Fisher's exact test and showed similar distributions. Linkage disequilibrium (LD) tests were performed in males for all pairs of loci and no significant associations were detected. Parameters of forensic interest were also estimated.

  19. Paradoxical Personality and Academic Achievement in College Students From Buenos Aires.

    PubMed

    Freiberg Hoffmann, Agustín; Fernández Liporace, María Mercedes

    2015-11-01

    This paper presents a study on paradoxical personality, defined as a distinctive feature in creative persons, developed with 350 college students from Buenos Aires. Goals aimed at describing and analysing possible significant differences of paradoxical traits in students from diverse majors representing seven different fields of study, and examining the relationship between each bipolar trait and academic achievement. The sample was composed of 7 groups (n = 50 by group) representing fields of study typically offered in public universities, Biology, Computer Science, Engineering, Law, Nutrition, Psychology, and History of Art. Analyses by career provided descriptive information about students of these majors, concerning their paradoxical personality profiles. Correlational studies verified significant associations between academic achievement and most paradoxical traits in majors such as Computer Science, Nutrition and Psychology. Results are discussed regarding practical outcomes and teaching programs.

  20. Population data for DXS6800, DXS101 and DXS8377 loci from Buenos Aires (Argentina).

    PubMed

    Noseda, Pablo A; Kenline, Jaclyn; Manning, Samantha; Gangitano, David A

    2013-07-01

    The X-chromosomal short tandem repeats (X-STRs) DXS6800, DXS101 and DXS8377 were analysed in a population sample from Buenos Aires (Argentina) using a polymerase chain reaction (PCR) multiplex approach with fluorescent detection. We present allele frequencies for these loci in a population comprising 113 women and 99 men. The Hardy-Weinberg equilibrium (HWE) was tested in the female sample and no significant deviations were observed. The homogeneity of allele frequencies of men and women was compared by the Fisher's exact test and showed similar distributions. Linkage disequilibrium (LD) tests were performed in males for all pairs of loci and no significant associations were detected. Parameters of forensic interest were also estimated. PMID:23756526

  1. First record of Sarcoramphus Dumeril 1806 (Ciconiiformes: Vulturidae) from the Pleistocene of Buenos Aires province, Argentina

    NASA Astrophysics Data System (ADS)

    Noriega, Jorge I.; Areta, Juan I.

    2005-10-01

    The finding of an incomplete specimen of Sarcoramphus papa (Linné 1758), commonly known as the King Vulture, from the locality of Camet Norte (Buenos Aires province) in Argentina is reported. The bearing level is referred to the Late Pleistocene Santa Clara Formation (Late Lujanian). Sarcoramphus papa is actually a dweller of undisturbed areas of tropical rainforest and humid tropical and subtropical forests in Central and South America, ranging from Mexico to northern Argentina. It is rarely found in large open areas such as steppes and grasslands and/or habitats with extremely low temperatures in winter. The fossiliferous locality is located geographically more than 700 km south of the species' southern distributional limit and thus far away from suitable habitats for its survival. This fossil record contradicts previous paleoenvironmental reconstructions for the same place and time, suggesting a mixed community with some arboreal structures capable of holding this taxon.

  2. Functional Foods and Nutraceuticals in a Market of Bolivian Immigrants in Buenos Aires (Argentina)

    PubMed Central

    Pochettino, María Lelia; Puentes, Jeremías P.; Buet Costantino, Fernando; Arenas, Patricia M.; Ulibarri, Emilio A.; Hurrell, Julio A.

    2012-01-01

    This paper presents the results of a research in urban ethnobotany, conducted in a market of Bolivian immigrants in the neighborhood of Liniers, Ciudad Autónoma de Buenos Aires (Argentina). Functional foods and nutraceuticals belonging to 50 species of 18 families, its products, and uses were recorded. Some products are exclusive from the Bolivian community; others are frequent within the community, but they are also available in the general commercial circuit; they are introduced into it, generally, through shops called dietéticas (“health-food stores”), where products associated with the maintenance of health are sold. On this basis, the traditional and nontraditional components of the urban botanical knowledge were evaluated as well as its dynamics in relation to the diffusion of the products. Both the framework and methodological design are innovative for the studies of the urban botanical knowledge and the traditional markets in metropolitan areas. PMID:22203866

  3. Late Paleozoic transpression in Buenos Aires and northeast Patagonia ranges, Argentina

    NASA Astrophysics Data System (ADS)

    Rossello, E. A.; Massabie, A. C.; López-Gamundí, O. R.; Cobbold, P. R.; Gapais, D.

    1997-12-01

    Paleozoic sediments are present in three regions in eastern central Argentina: (1) the Sierras Australes of Buenos Aires, (2) Sierras Septentrionales of Buenos Aires and (3) Northeast Patagonia. All of these deposits share a common deformational imprint imparted by late Paleozoic Gondwanan deformation. Exposures of these rocks are scattered, variably deformed, and isolated by younger sediments deposited in basins related to the Mesozoic through Tertiary opening of the South Atlantic such as the offshore Colorado Basin. The Sierras Australes of Buenos Aires outcrops are the best preserved. They are mostly located along the Sierras Australes foldbelt, with minor outliers distributed in the adjacent Claromec-basin. The Tunas Formation (early-early late? Permian) is the uppermost unit of the Pillahuincó Group (late Carboniferous-Permian) and is crucial to the understanding of the tectono-sedimentary evolution of the region during the late Paleozoic. The underlying units of the Pillahuincó Group (Sauce Grande, Piedra Azul and Bonete Formations) exhibit a depositional and compositional history characterized by glaciomarine sedimentation and postglacial transgression. They are also characterized by rather uniform quartz-rich compositions indicative of a cratonic provenance from the La Plata craton to the NE. In contrast, the sandstone-rich Tunas Formation has low quartz contents, and abundant volcanic and metasedimentary fragments; paleocurrents are consistently from the SW. Glassrich tuffs are interbedded with sandstone in the upper half of the Tunas Formation. The age of the deformation in the Sierras Australes is Permian and early-middle Triassic. This is based on metamorphic events indicated by formation of illite at 282 ± 3 Ma, 273 ± 8 Ma, 265 ± 3 Ma, and 260 ± 3 Ma ( {K}/{Ar} illite) in the Silurian Curamalal Group. Evidence of syntectonic magmatism is provided by a radiometric date of 245 ± 12 Ma ( {K}/{Ar} hornblende) for the López Lecube Granite

  4. Paradoxical Personality and Academic Achievement in College Students From Buenos Aires

    PubMed Central

    Freiberg Hoffmann, Agustín; Fernández Liporace, María Mercedes

    2015-01-01

    This paper presents a study on paradoxical personality, defined as a distinctive feature in creative persons, developed with 350 college students from Buenos Aires. Goals aimed at describing and analysing possible significant differences of paradoxical traits in students from diverse majors representing seven different fields of study, and examining the relationship between each bipolar trait and academic achievement. The sample was composed of 7 groups (n = 50 by group) representing fields of study typically offered in public universities, Biology, Computer Science, Engineering, Law, Nutrition, Psychology, and History of Art. Analyses by career provided descriptive information about students of these majors, concerning their paradoxical personality profiles. Correlational studies verified significant associations between academic achievement and most paradoxical traits in majors such as Computer Science, Nutrition and Psychology. Results are discussed regarding practical outcomes and teaching programs. PMID:27247680

  5. Functional foods and nutraceuticals in a market of bolivian immigrants in Buenos Aires (Argentina).

    PubMed

    Pochettino, María Lelia; Puentes, Jeremías P; Buet Costantino, Fernando; Arenas, Patricia M; Ulibarri, Emilio A; Hurrell, Julio A

    2012-01-01

    This paper presents the results of a research in urban ethnobotany, conducted in a market of Bolivian immigrants in the neighborhood of Liniers, Ciudad Autónoma de Buenos Aires (Argentina). Functional foods and nutraceuticals belonging to 50 species of 18 families, its products, and uses were recorded. Some products are exclusive from the Bolivian community; others are frequent within the community, but they are also available in the general commercial circuit; they are introduced into it, generally, through shops called dietéticas ("health-food stores"), where products associated with the maintenance of health are sold. On this basis, the traditional and nontraditional components of the urban botanical knowledge were evaluated as well as its dynamics in relation to the diffusion of the products. Both the framework and methodological design are innovative for the studies of the urban botanical knowledge and the traditional markets in metropolitan areas. PMID:22203866

  6. Paradoxical Personality and Academic Achievement in College Students From Buenos Aires.

    PubMed

    Freiberg Hoffmann, Agustín; Fernández Liporace, María Mercedes

    2015-11-01

    This paper presents a study on paradoxical personality, defined as a distinctive feature in creative persons, developed with 350 college students from Buenos Aires. Goals aimed at describing and analysing possible significant differences of paradoxical traits in students from diverse majors representing seven different fields of study, and examining the relationship between each bipolar trait and academic achievement. The sample was composed of 7 groups (n = 50 by group) representing fields of study typically offered in public universities, Biology, Computer Science, Engineering, Law, Nutrition, Psychology, and History of Art. Analyses by career provided descriptive information about students of these majors, concerning their paradoxical personality profiles. Correlational studies verified significant associations between academic achievement and most paradoxical traits in majors such as Computer Science, Nutrition and Psychology. Results are discussed regarding practical outcomes and teaching programs. PMID:27247680

  7. [Molecular detection of Saint Louis encephalitis virus in mosquitoes in Buenos Aires].

    PubMed

    Beltrán, Fernando J; Bechara, Yamila I; Guido, Guillermo G; Cicuttin, Gabriel L; Beaudoin, Juan B; Gury Dohmen, Federico E

    2014-01-01

    During March 2013 a population of eared doves (Zenaida auriculata) was established in the center of City of Buenos Aires. Considering the role of these birds as host competent for Saint Louis encephalitis virus (SLEV), a CDC light trap was put in place to perform entomologic surveillance. During this month 5 pools of mosquitoes (n = 48) were collected and taxonomically determined. Three of them were classified as Culex pipiens (n = 10) and the other two were Culex spp. (n = 38). In this case, the mosquitoes species could not be determined due to that individuals were damaged. One of the Culex spp. pool was found to be positive for Saint Louis encephalitis virus by molecular techniques. This was then sequenced and classified as genotype III.

  8. HIV status disclosure among infected men who have sex with men (MSM) in Buenos Aires, Argentina.

    PubMed

    Carballo-Diéguez, Alex; Balán, Iván C; Dolezal, Curtis; Ibitoye, Mobolaji; Pando, María A; Marone, Rubén; Barreda, Victoria; Avila, María Mercedes

    2013-12-01

    Five hundred men who have sex with men were recruited in Buenos Aires using respondent driven sampling. Of them, 46 respondents (24 of them not gay identified) who were HIV infected were asked questions on serodisclosure. The sample was characterized by indicators of low socioeconomic status. Most of the respondents reported being in good to excellent health despite 42% of them having been diagnosed with AIDS. Only 10% of respondents had not disclosed their serostatus to at least one person. Coworkers and lovers or main sexual partners were those most likely to know the respondents' serostatus. Reactions to disclosure were for the most part supportive. Those who had not disclosed anticipated less favorable reactions than those who had disclosed. No significant differences were observed between gay and non-gay identified respondents. The progressive social environment of Argentina that includes federal laws recognizing gay marriage may contribute to create a climate favorable for serostatus disclosure. PMID:24245593

  9. Atmospheric control of Aedes aegypti populations in Buenos Aires (Argentina) and its variability

    NASA Astrophysics Data System (ADS)

    de Garín, A.; Bejarán, R. A.; Carbajo, A. E.; de Casas, S. C.; Schweigmann, N. J.

    The mosquito Aedes aegypti is the main urban vector responsible for the transmission of dengue fever and dengue hemorrhagic fever. The city of Buenos Aires, Argentina, is located at the southern end of the world distribution of the species. The population abundance of Ae. aegypti is mainly regulated by environmental factors. We calculated the potential number of times that a female could lay eggs during its mean life expectancy, based on potential egg production and daily meteorological records. The model considers those variables implying physical hazard to the survival of Ae. aegypti, mosquito flying activity and oviposition. The results, obtained after calibration and validation of the model with field observations, show significant correlation (P<0.001) for different lags depending on the life stage. From these results, more favorable atmospheric conditions for Ae. aegypti reproduction (linked to the urban climatic change) can be observed. The climatic variability in the last decade resembles conditions at the end of 19th century.

  10. HIV testing practices among men who have sex with men in Buenos Aires, Argentina.

    PubMed

    Carballo-Diéguez, Alex; Balán, Iván C; Dolezal, Curtis; Pando, María A; Marone, Rubén; Barreda, Victoria; Avila, María Mercedes

    2014-01-01

    The objective of the study was to explore HIV-testing practices among men who have sex with men (MSM) in Buenos Aires, Argentina, in light of current international health guidelines that recommend frequent HIV testing for MSM who engage in high-risk behavior. Participants, who were recruited using respondent-driven sampling (RDS), were 500 mostly young, nongay-identified MSM of low socioeconomic status, high levels of unemployment, living mainly in the less-affluent areas surrounding Buenos Aires, and lacking health insurance. They provided blood samples for HIV testing and responded to a Computer Assisted Self Interview. Fifty-two percent had never been tested for HIV, and 20% had been tested only once; 17% were found to be HIV infected, of whom almost half were unaware of their status. Main reasons for never having tested previously were: not feeling at risk, fear of finding out results, and not knowing where to get tested. Among those previously tested, men had been tested a median of 2 times with their most recent test having occurred a median of 2.7 years prior to study enrollment. Of those who had not tested positive before entering the study, only 41% returned for their results. HIV testing was infrequent and insufficient for early detection of infection, entry into treatment, and protection of sexual partners. This was particularly the case among nongay-identified MSM. Testing campaigns should aim to help MSM become aware of their risk behavior, decrease fear of testing by explaining available treatment resources and decreasing the stigma associated with HIV, and by publicizing information about free and confidential testing locations. Rapid HIV testing should be made available to eliminate the need for a return visit and make results immediately available to individuals who are tested. PMID:23659314

  11. Dengue Virus 1 in Buenos Aires from 1999 to 2010: Towards Local Spread

    PubMed Central

    Tittarelli, Estefanía; Mistchenko, Alicia S.

    2014-01-01

    Dengue virus (DENV) is a public health problem representing the most important arthropod-borne viral disease in humans. In Argentina, Northern provinces have reported autochthonous cases since 1997, though these outbreaks have originated in bordering countries, where co-circulation of more than one serotype has been reported. In the last decade, imported dengue cases have been reported in Buenos Aires, the urban area of Argentina with the highest population density. In 2009, a dengue outbreak affected Buenos Aires and, for the first time, local transmission was detected. All cases of this outbreak were caused by DENV-1. In this report, we present the full-length sequences of 27 DENV-1 isolates, corresponding to imported cases of 1999–2000, as well as local and imported cases of the 2009 and 2010 outbreaks. We analyzed their phylogenetic and phylodynamic relationships and their global and local spread. Additionally, we characterized their genomic and phenotypic features. All cases belonged to DENV-1 genotype V. The most recent ancestor for this genotype was dated ∼1934, whereas that for the 2009 outbreak was dated ∼2007. The mean rates of nucleotide substitution were 4.98E-4 and 8.53E-4 subs./site/yr, respectively. We inferred an introduction from Paraguay in 1999–2000 and mainly from Venezuela during 2009–2010. Overall, the number of synonymous substitutions per synonymous site significantly exceeded the number of non-synonymous substitutions per site and 12 positively selected sites were detected. These analyses could contribute to a better understanding regarding spread and evolution of this pathogen in the Southern Cone of South America. PMID:25343372

  12. Reproductive performance of dairy farms in western Buenos Aires province, Argentina.

    PubMed

    Ferreira, Gonzalo

    2013-01-01

    The objective of this study was to describe the reproductive performance of 23 grazing-based dairy farms from western Buenos Aires province in Argentina. The data set included data from the breeding season starting in May 2011 and ending in March 2012. Submission, conception, and pregnancy rates ranged from 42.4 to 70.2%, 20.1 to 44.9%, and 10.3 to 24.5%, respectively. No correlation was observed between conception and submission rates, suggesting that dairy farms with poor submission rates but with relatively high conception rates might increase pregnancy rates by simply putting more effort into increasing estrus detection and submission rates. Decreases in submission and conception rates were observed among 21-d cycles, indicating seasonal variation. A greater number of cows in estrus at the beginning of the breeding period could have facilitated estrus detection and therefore increased submission rates. In addition, restarting the breeding activities with timed artificial insemination programs may explain the highest submission rates at the beginning of the breeding period. A first decrease of 5.1 percentage units in conception rate was observed during the spring (October-November) and an additional decrease of 2.4 percentage units in conception rate was observed during the summer (January-February). Decreases in conception rates could be related to high intakes of high-protein diets, heat stress, or a combination of both. Attenuating heat stress during the summer may be critical for maximizing conception rates in grazing systems from western Buenos Aires province.

  13. Increasing trends in primary NNRTI resistance among newly HIV-1-diagnosed individuals in Buenos Aires, Argentina

    PubMed Central

    Rodriguez-Rodrigues, Nahuel; Duran, Adriana; Bouzas, María Belen; Zapiola, Ines; Vila, Marcelo; Indyk, Debbie; Bissio, Emiliano; Salomon, Horacio; Dilernia, Dario A

    2013-01-01

    Objective Our objective was to estimate primary resistance in an urban setting in a developing country characterized by high antiretroviral (ARV) coverage over the diagnosed population and also by an important proportion of undiagnosed individuals, in order to determine whether any change in primary resistance occurred in the past five years. Design We carried out a multi-site resistance surveillance study according to WHO HIV resistance guidelines, using a weighted sampling technique based on annual HIV case reports per site. Methods Blood samples were collected from 197 drug-naive HIV-1-infected individuals diagnosed between March 2010 and August 2011 at 20 HIV voluntary counselling and testing centres in Buenos Aires. Clinical records of enrolled patients at the time of diagnosis were compiled. Viral load and CD4 counts were performed on all samples. The pol gene was sequenced and the resistance profile determined. Phylogenetic analysis was performed by neighbour-joining (NJ) trees and bootscanning analysis. Results We found that 12 (7.9%) of the 152 successfully sequenced samples harboured primary resistance mutations, of which K103N and G190A were the most prevalent. Non-nucleoside reverse transcriptase inhibitors (NNRTI) resistance mutations were largely the most prevalent (5.9%), accounting for 75% of all primary resistance and exhibiting a significant increase (p=0.0072) in prevalence during the past 10 years as compared to our previous study performed in 1997–2000 and in 2003–2005. Nucleoside reverse transcriptase inhibitor (NRTI) and protease inhibitor primary resistance were low and similar to the one previously reported. Conclusions Levels of primary NNRTI resistance in Buenos Aires appear to be increasing in the context of a sustained ARV coverage and a high proportion of undiagnosed HIV-positive individuals. PMID:24093951

  14. [Air contamination in the Autonomous City of Buenos Aires: the current risk or future climate change, a false option].

    PubMed

    Abrutzky, Rosana; Dawidowski, Laura; Murgida, Ana; Natenzon, Claudia Eleonor

    2014-09-01

    Based on the theoretical framework of environmental risk, this article discusses the management of air quality in the Autonomous City of Buenos Aires in relation to current and potential impacts of toxic gases and global climate change on the health of the population. Information on historical and current management of the air was linked to the results of the South American Emissions, Megacities and Climate research project to assess danger, exposure, vulnerability and uncertainty as the dimensions of risk. By contextualizing public policies developed in recent decades on this subject, it was possible to identify emerging configurations of risk and uncertainties as accelerators of social vulnerability. On the one hand, the fact that there is a positive correlation between mortality, changes in temperature and air pollution was confirmed. On the other hand, it became clear that there is a disconnect between air quality management and health care management, while limitations were found in the proposed mitigation measures relating to emissions of greenhouse gases produced by fuel, revealing uncertainties regarding their efficacy.

  15. [Reading the 'natural book': notes towards a history of anatomical and surgical studies in Buenos Aires (1870-1895)].

    PubMed

    Souza, Pablo; Hurtado, Diego

    2010-12-01

    The article explores transformations in anatomical and surgical studies within the school of medical thought in Buenos Aires in the latter half of the nineteenth century, focusing on the tension between how this knowledge should be conveyed and the changing surgical worldviews of the most visible medical groups of the 1870s. The influence of European surgical traditions is analyzed within this context, particularly the influence of the Paris tradition, which can be linked to study abroad by young Ignacio Pirovano, who would later become professor of anatomy and major surgery in Buenos Aires. The article pays special attention to changes in forms of experiments and in the new material conditions afforded through the introduction of antiseptic surgery, some types of anesthesia, and bone resection. PMID:21461456

  16. [Collectors of historical, archeological, and natural science objects at municipal museums in Buenos Aires province, Argentina, during the 1950s].

    PubMed

    Pupio, María Alejandra

    2005-01-01

    Through reference to the creation and expansion of municipal museums in the province of Buenos Aires during the 1950s, the article explores some aspects of how archeological collections are compiled. The collections under study came from private hands, having been gathered by collectors who relinquished them so these museums could be formed. At the same time that these collections became public, the collectors themselves became responsible for them in the role of directors of the new institutes. Within this context, the collectors established institutional relations that allowed them to devise common strategies concerning the receipt, selection, and exhibition of archeological collections. The result was the shaping of a network of solidarity in the southern part of Buenos Aires province.

  17. Simulation of regional-scale groundwater flow in the Azul River basin, Buenos Aires Province, Argentina

    NASA Astrophysics Data System (ADS)

    Varni, Marcelo R.; Usunoff, Eduardo J.

    A three-dimensional modular model (MODFLOW) was used to simulate groundwater flow in the Azul River basin, Buenos Aires Province, Argentina, in order to assess the correctness of the conceptual model of the hydrogeological system. Simulated heads satisfactorily match observed heads in the regional water-table aquifer. Model results indicate that: (1) groundwater recharge is not uniform throughout the region but is best represented by three recharge rates, decreasing downgradient, similar to the distribution of soils and geomorphological characteristics; and (2) evapotranspiration rates are larger than previous estimates, which were made by using the Thornthwaite-Mather method. Evapotranspiration rates estimated by MODFLOW agree with results of independent studies of the region. Model results closely match historical surface-flow records, thereby suggesting that the model description of the aquifer-river relationship is correct. Résumé Un modèle modulaire tridimensionnel (MODFLOW) a été utilisé pour simuler les écoulements souterrains dans le bassin de la rivière Azul (Province de Buenos Aires, Argentine), dans le but d'évaluer la justesse du modèle conceptuel du système hydrogéologique. La piézométrie simulée s'ajuste de façon satisfaisante à celle observée pour l'ensemble de la nappe. Les résultats du modèle indiquent que: (1) la recharge de la nappe n'est pas uniforme sur toute la région, mais qu'elle est mieux approchée par trois valeurs différentes, décroissant vers l'aval-gradient, en suivant la même distribution que les sols et les caractéristiques géomorphologiques et (2) l'évapotranspiration est nettement plus importante que prévu initialement à partir de la méthode de Thornthwaite-Mather. Les valeurs d'évapotranspiration fournies par MODFLOW concordent bien avec les résultats d'autres études portant sur la région. Les résultats du modèle reproduisent convenablement les chroniques de débit des écoulements de surface

  18. Prediction of seasonal water-table fluctuations in La Pampa and Buenos Aires, Argentina

    NASA Astrophysics Data System (ADS)

    Tanco, Raúl; Kruse, Eduardo

    2001-07-01

    The fluctuation of the water table east of La Pampa province and northwest of Buenos Aires province, Argentina, influences agricultural production in the region because it is closely related to the alternation of dry and wet periods. Sea-surface temperature (SST) anomalies have been used as predictors to forecast atmospheric variables in different regions of the world. The objective of this work is to present a simple model to forecast seasonal rainfall using SST distribution in the Pacific Ocean as a predictor. Once the relationship between precipitation and water-table fluctuations was established, a methodology for the prediction of water-table fluctuations was developed. A good agreement between observed and predicted water-table fluctuations was found when estimating water-table fluctuations in the summer and autumn seasons. Résumé. Les fluctuations de la nappe à l'est de la province de La Pampa et au nord-ouest de la province de Buenos Aires (Argentine) influence la production agricole de la région parce qu'elle est étroitement liée à l'alternance de saisons sèches et humides. Les anomalies de la température de surface de l'océan (SST) ont été utilisées comme prédicteurs pour prévoir les variables atmosphériques dans différentes régions du monde. L'objectif de ce travail est de présenter un modèle simple de prévision des précipitations saisonnières en utilisant comme prédicteur la distribution des SST dans l'Océan Pacifique. Une fois que la relation entre les fluctuations des précipitations et celles de la nappe a été établie, une méthodologie de prédiction des variations de la nappe a été mise au point. Un bon accord entre les variations de la nappe observées et celles prédites a été trouvé pour les estimations des variations de nappe en été et en automne. Resumen. La fluctuación del nivel freático al este de la provincia de La Pampa y al nordeste de la de Buenos Aires (Argentina) repercute en la producción agr

  19. [Migrants from bordering countries in the labor force of the metropolitan area of Buenos Aires, 1980-1996].

    PubMed

    Maguid, A

    1997-04-01

    Data from the 1991 Argentine census indicate that migrants from neighboring countries at that date were maintaining patterns of entry into the labor force of Buenos Aires consistent with past tendencies. Migrants tended to be employed in less skilled manufacturing industries, construction, commerce, and domestic work, often filling positions rejected by the native population because of low wages and poor working conditions. Profound changes in the Argentine economy since 1991 have included rising unemployment and underemployment and a loss of productive jobs in industry and construction. A comparison of the occupational structure of migrants from neighboring countries and of the total population for the years 1980, 1991, and 1996 demonstrates that important changes in sectorial employment have occurred among both the native and immigrant populations, with the immigrant population increasingly relegated to ever smaller sectors of the labor market offering less attractive employment. Nearly half of the 841,697 persons immigrating in 1991 from countries bordering Argentina settled in the Buenos Aires metropolitan region, comprising 42.8% of foreigners in the metropolitan area and 3.7% of the total regional population. Of the population from border countries residing in Buenos Aires, 43% are Paraguayan, 28% Uruguayan, 15% Bolivian, 12% Chilean, and 2% Brazilian. The unemployment rate in Buenos Aires fluctuated between 4% and 6% during 1974-92, but it rose to 10.6% in 1993 and then to 18% in 1996. The underemployment rate rose from 4.6% in 1983 to 8.2% in 1993 and 12.6% in 1996.

  20. Molecular characterization of Rickettsia massiliae and Anaplasma platys infecting Rhipicephalus sanguineus ticks and domestic dogs, Buenos Aires (Argentina).

    PubMed

    Cicuttin, Gabriel L; Brambati, Diego F; Rodríguez Eugui, Juan I; Lebrero, Cecilia González; De Salvo, María N; Beltrán, Fernando J; Gury Dohmen, Federico E; Jado, Isabel; Anda, Pedro

    2014-09-01

    Rickettsioses, ehrlichioses and anaplasmoses are emerging diseases that are mainly transmitted by arthropods and that affect humans and animals. The aim of the present study was to use molecular techniques to detect and characterize those pathogens in dogs and ticks from Buenos Aires city. We studied 207 Rhipicephalus sanguineus ticks and 52 canine blood samples from poor neighborhoods of Buenos Aires city. The samples were molecularly screened for the genera Rickettsia, Ehrlichia, and Anaplasma by PCR and sequencing. DNA of Rickettsia massiliae (3.4%) and Anaplasma platys (13.5%) was detected in ticks and blood samples, respectively. For characterization, the positive samples were subjected to amplification of a fragment of the 190-kDa outer membrane protein gene (spotted fever group rickettsiae) and a fragment of the groESL gene (specific for A. platys). A phylogenetic tree was constructed using the neighbor-joining method, revealing that the sequences were closely related to those of strains from other geographic regions. The results indicate that human and animal pathogens are abundant in dogs and their ticks in Buenos Aires city and portray the potentially high risk of human exposure to infection with these agents, especially in poor neighborhoods, where there is close contact with animals in an environment of poor health conditions.

  1. Genotypes of Leptospira spp. strains isolated from dogs in Buenos Aires, Argentina.

    PubMed

    Grune Loffler, Sylvia; Passaro, Diego; Samartino, Luis; Soncini, Analía; Romero, Graciela; Brihuega, Bibiana

    2014-01-01

    Leptospirosis is an infectious disease of wide global distribution, which is endemic in Argentina. The objective of this study was to obtain the genetic profiles of Leptospira spp. strains isolated from clinical cases of dogs in the province of Buenos Aires by the multiple-locus variable-number tandem repeat analysis (MLVA). Eight isolated canine strains were genotyped by MLVA, obtaining the identical profile of Leptospira interrogans serovar Canicola Hond Utrecht IV in the strains named Dogy and Mayo. The strains named Bel, Sarmiento, La Plata 4581 and La Plata 5478 were identical to the profile of the genotype of L. interrogans serovar Portlandvere MY 1039.The strain named Avellaneda was identical to the genotype profile of L. interrogans serovar Icterohaemorrhagiae RGA and the strain named SB had the same profile as the L. interrogans serovar Pomona Baires genotype and was similar to the profile of serovar Pomona Pomona genotype. It would be useful to include a larger number of isolates from different dog populations in various provinces of Argentina and to characterize the genetic profiles of the strains circulating in the country. The information obtained will be useful for the control of leptospirosis in the dog population.

  2. Prevalence of the amphibian chytrid fungus (Batrachochytrium dendrobatidis) at Buenos Aires National Wildlife Refuge, Arizona, USA

    USGS Publications Warehouse

    Sigafus, Brent H.; Hossack, Blake R.; Muths, Erin L.; Schwalbe, Cecil R.

    2014-01-01

    Information on disease presence can be of use to natural resource managers, especially in areas supporting threatened and endangered species that occur coincidentally with species that are suspected vectors for disease. Ad hoc reports may be of limited utility (Muths et al. 2009), but a general sense of pathogen presence (or absence) can inform management directed at T&E species, especially in regions where disease is suspected to have caused population declines (Bradley et al. 2002). The Chiricahua Leopard Frog (Lithobates chiricahuensis), a species susceptible to infection by the amphibian chytrid fungus (Batrachochytrium dendrobatidis, Bd) (Bradley et al. 2002), and the non-native, invasive American Bullfrog (L. catesbeianus), a suspected vector for chytridiomycosis (Schloegel et al. 2012, Gervasi et al. 2013), both occur at Buenos Aires National Wildlife Refuge (BANWR) and surrounding lands in southern Arizona. Efforts to eradicate the bullfrog from BANWR began in 1997 (Suhre, 2010). Eradication from the southern portion of BANWR was successful by 2008 but the bullfrog remains present at the Arivaca Cienega and in areas immediately adjacent to the refuge (Fig. 1). Curtailing the re-invasion of the bullfrog into BANWR will require vigilance as to ensure the health of Chiricahua Leopard Frog populations.

  3. Ecotoxicological and Genotoxic Evaluation of Buenos Aires City (Argentina) Hospital Wastewater

    PubMed Central

    Juárez, Ángela Beatriz; Dragani, Valeria; Saenz, Magalí Elizabeth; Moretton, Juan

    2014-01-01

    Hospital wastewater (HWW) constitutes a potential risk to the ecosystems and human health due to the presence of toxic and genotoxic chemical compounds. In the present work we investigated toxicity and genotoxicity of wastewaters from the public hospital of Buenos Aires (Argentina). The effluent from the sewage treatment plant (STP) serving around 10 million inhabitants was also evaluated. The study was carried out between April and September 2012. Toxicity and genotoxicity assessment was performed using the green algae Pseudokirchneriella subcapitata and the Allium cepa test, respectively. Toxicity assay showed that 55% of the samples were toxic to the algae (%I of growth between 23.9 and 54.8). The A. cepa test showed that 40% of the samples were genotoxic. The analysis of chromosome aberrations (CA) and micronucleus (MN) showed no significant differences between days and significant differences between months. The sample from the STP was not genotoxic to A. cepa but toxic to the algae (%I = 41%), showing that sewage treatment was not totally effective. This study highlights the need for environmental control programs and the establishment of advanced and effective effluent treatment plants in the hospitals, which are merely dumping the wastewaters in the municipal sewerage system. PMID:25214834

  4. Ecotoxicological and genotoxic evaluation of Buenos Aires city (Argentina) hospital wastewater.

    PubMed

    Magdaleno, Anahí; Juárez, Angela Beatriz; Dragani, Valeria; Saenz, Magalí Elizabeth; Paz, Marta; Moretton, Juan

    2014-01-01

    Hospital wastewater (HWW) constitutes a potential risk to the ecosystems and human health due to the presence of toxic and genotoxic chemical compounds. In the present work we investigated toxicity and genotoxicity of wastewaters from the public hospital of Buenos Aires (Argentina). The effluent from the sewage treatment plant (STP) serving around 10 million inhabitants was also evaluated. The study was carried out between April and September 2012. Toxicity and genotoxicity assessment was performed using the green algae Pseudokirchneriella subcapitata and the Allium cepa test, respectively. Toxicity assay showed that 55% of the samples were toxic to the algae (%I of growth between 23.9 and 54.8). The A. cepa test showed that 40% of the samples were genotoxic. The analysis of chromosome aberrations (CA) and micronucleus (MN) showed no significant differences between days and significant differences between months. The sample from the STP was not genotoxic to A. cepa but toxic to the algae (%I = 41%), showing that sewage treatment was not totally effective. This study highlights the need for environmental control programs and the establishment of advanced and effective effluent treatment plants in the hospitals, which are merely dumping the wastewaters in the municipal sewerage system.

  5. Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina.

    PubMed

    Scialfa, E; Bolpe, J; Bardón, J C; Ridao, G; Gentile, J; Gallicchio, O

    2010-01-01

    The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.

  6. A Pleistocene (MIS 5e) mollusk assemblage from Ezeiza (Buenos Aires Province, Argentina)

    NASA Astrophysics Data System (ADS)

    Martínez, Sergio; Julia del Río, Claudia; Rojas, Alejandra

    2016-10-01

    A fossil assemblage collected around 3.5 m amsl from Ezeiza, Buenos Aires province, have AMS 14C ages of ca. 33,000 to ca. 40,000 yr BP, whereas in the literature is a report of a conventional 14C age of >43,000 yr BP. An OSL age from the overlying deposit corresponds to ca. 22,000 yr. The samples contain marine fossils: mollusks, balanids and corals (Astrangia). La Coronilla (Uruguay, attributed to MIS 5e) is the locality most related to Ezeiza faunistically, despite is not the nearest one. In consequence, the relationship should be addressed to a more similar age and environment than others. The fauna indicates a higher water temperature than today. In Ezeiza exclusively cold water taxa are absent, and we found seven warm taxa with their southern distribution limit displaced northwards today, plus other six at their southern distribution limit. Around 60% of all the species and more than 70% of the individuals are of warm-temperate waters. In sum, although prima facie the numerical ages would locate the deposit in MIS3, faunistic, temperature, and height evidences show that the Ezeiza mollusk assemblage belong to MIS5e. A stronger than presently Brazil warm current, reaching Southern latitudes, may explain the changes in geographical ranges.

  7. Urban Chagas disease in children and women in primary care centres in Buenos Aires, Argentina.

    PubMed

    Moscatelli, Guillermo; Berenstein, Ada; Tarlovsky, Ana; Siniawski, Susana; Biancardi, Miguel; Ballering, Griselda; Moroni, Samanta; Schwarcz, Marta; Hernández, Susana; García-Bournissen, Facundo; Cozzi, Andrés Espejo; Freilij, Héctor; Altcheh, Jaime

    2015-08-01

    The primary objective of this study was to estimate the prevalence of this disease in women of childbearing age and children treated at health centres in underserviced areas of the city of Buenos Aires. Demographic and Chagas disease status data were collected. Samples for Chagas disease serology were obtained on filter paper and the reactive results were confirmed with conventional samples. A total of 1,786 subjects were screened and 73 positive screening results were obtained: 17 were from children and 56 were from women. The Trypanosoma cruzi infection risk was greater in those individuals who had relatives with Chagas disease, who remember seeing kissing bugs, who were of Bolivian nationality or were born in the Argentine province of Santiago del Estero. The overall prevalence of Chagas disease was 4.08%. Due to migration, Chagas disease is currently predominantly urban. The observed prevalence requires health programme activities that are aimed at urban children and their mothers. Most children were infected congenitally, which reinforces the need for Chagas disease screening of all pregnant women and their babies in Argentina. The active search for new cases is important because the appropriate treatment in children has a high cure rate.

  8. Genotypes of Leptospira spp. strains isolated from dogs in Buenos Aires, Argentina.

    PubMed

    Grune Loffler, Sylvia; Passaro, Diego; Samartino, Luis; Soncini, Analía; Romero, Graciela; Brihuega, Bibiana

    2014-01-01

    Leptospirosis is an infectious disease of wide global distribution, which is endemic in Argentina. The objective of this study was to obtain the genetic profiles of Leptospira spp. strains isolated from clinical cases of dogs in the province of Buenos Aires by the multiple-locus variable-number tandem repeat analysis (MLVA). Eight isolated canine strains were genotyped by MLVA, obtaining the identical profile of Leptospira interrogans serovar Canicola Hond Utrecht IV in the strains named Dogy and Mayo. The strains named Bel, Sarmiento, La Plata 4581 and La Plata 5478 were identical to the profile of the genotype of L. interrogans serovar Portlandvere MY 1039.The strain named Avellaneda was identical to the genotype profile of L. interrogans serovar Icterohaemorrhagiae RGA and the strain named SB had the same profile as the L. interrogans serovar Pomona Baires genotype and was similar to the profile of serovar Pomona Pomona genotype. It would be useful to include a larger number of isolates from different dog populations in various provinces of Argentina and to characterize the genetic profiles of the strains circulating in the country. The information obtained will be useful for the control of leptospirosis in the dog population. PMID:25444128

  9. Urban Chagas disease in children and women in primary care centres in Buenos Aires, Argentina

    PubMed Central

    Moscatelli, Guillermo; Berenstein, Ada; Tarlovsky, Ana; Siniawski, Susana; Biancardi, Miguel; Ballering, Griselda; Moroni, Samanta; Schwarcz, Marta; Hernández, Susana; García-Bournissen, Facundo; Cozzi, Andrés Espejo; Freilij, Héctor; Altcheh, Jaime

    2015-01-01

    The primary objective of this study was to estimate the prevalence of this disease in women of childbearing age and children treated at health centres in underserviced areas of the city of Buenos Aires. Demographic and Chagas disease status data were collected. Samples for Chagas disease serology were obtained on filter paper and the reactive results were confirmed with conventional samples. A total of 1,786 subjects were screened and 73 positive screening results were obtained: 17 were from children and 56 were from women. The Trypanosoma cruzi infection risk was greater in those individuals who had relatives with Chagas disease, who remember seeing kissing bugs, who were of Bolivian nationality or were born in the Argentine province of Santiago del Estero. The overall prevalence of Chagas disease was 4.08%. Due to migration, Chagas disease is currently predominantly urban. The observed prevalence requires health programme activities that are aimed at urban children and their mothers. Most children were infected congenitally, which reinforces the need for Chagas disease screening of all pregnant women and their babies in Argentina. The active search for new cases is important because the appropriate treatment in children has a high cure rate. PMID:26222020

  10. Social determinants of nonadherence to tuberculosis treatment in Buenos Aires, Argentina.

    PubMed

    Herrero, Maria Belen; Arrossi, Silvina; Ramos, Silvina; Braga, Jose Ueleres

    2015-09-01

    This study aimed to identify the individual and environmental determinants of nonadherence to tuberculosis (TB) treatment in selected districts in the Buenos Aires Metropolitan Area, in Argentina. We conducted a cross-sectional study using a hierarchical model. Using primary and secondary data, logistic regression was performed to analyze two types of determinants. The likelihood of nonadherence to treatment was greatest among male patients. The following factors led to a greater likelihood of nonadherence to treatment: patients living in a home without running water; head of household without medical insurance; need to use more than one means of transport to reach the health center; place of residence in an area with a high proportion of households connected to the natural gas network; place of residence in an area where a large proportion of families fall below the minimum threshold of subsistence capacity; place of residence in an area where a high proportion of households do not have flushing toilets and basic sanitation. Our results show that social and economic factors - related to both individual and environmental characteristics - influence adherence to TB treatment. PMID:26578022

  11. [Recent history: 12th International Conference on Cancer, Buenos Aires, Argentina, 1978].

    PubMed

    Spinelli, Hugo

    2014-04-01

    Using the approaches of history of the present, this article recovers the discussions surrounding the 12th International Conference on Cancer carried out in Buenos Aires in 1978, in reaction to which Georges Périès organized a "counter-conference" in Paris. In order to understand this discussion, the political situation of the time is described, as is the state of human rights at the time in Argentina, the role of the media - in particular the newspapers La Nación and Clarín and the magazine Gente - and the institutional position adopted by the National Academy of Medicine, as expressed in a letter sent to the presidents of the primary scientific societies of the world. The letter is reprinted in this text as a documentary source, taken from Memoria: Año 1978 (Presidencia de Dr. José E. Rivarola) [Acta: Year 1978 (Presidency of Dr. José E. Rivarola)]. The framework of the discussion makes reference to science's social policy versus science's supposed neutrality and the role of scientific societies. PMID:24823605

  12. Urban Chagas disease in children and women in primary care centres in Buenos Aires, Argentina.

    PubMed

    Moscatelli, Guillermo; Berenstein, Ada; Tarlovsky, Ana; Siniawski, Susana; Biancardi, Miguel; Ballering, Griselda; Moroni, Samanta; Schwarcz, Marta; Hernández, Susana; García-Bournissen, Facundo; Cozzi, Andrés Espejo; Freilij, Héctor; Altcheh, Jaime

    2015-08-01

    The primary objective of this study was to estimate the prevalence of this disease in women of childbearing age and children treated at health centres in underserviced areas of the city of Buenos Aires. Demographic and Chagas disease status data were collected. Samples for Chagas disease serology were obtained on filter paper and the reactive results were confirmed with conventional samples. A total of 1,786 subjects were screened and 73 positive screening results were obtained: 17 were from children and 56 were from women. The Trypanosoma cruzi infection risk was greater in those individuals who had relatives with Chagas disease, who remember seeing kissing bugs, who were of Bolivian nationality or were born in the Argentine province of Santiago del Estero. The overall prevalence of Chagas disease was 4.08%. Due to migration, Chagas disease is currently predominantly urban. The observed prevalence requires health programme activities that are aimed at urban children and their mothers. Most children were infected congenitally, which reinforces the need for Chagas disease screening of all pregnant women and their babies in Argentina. The active search for new cases is important because the appropriate treatment in children has a high cure rate. PMID:26222020

  13. Well site conditions associated with nitrate contamination in a multilayer semiconfined aquifer of Buenos Aires, Argentina

    NASA Astrophysics Data System (ADS)

    Carbó, L. I.; Flores, M. C.; Herrero, M. A.

    2009-06-01

    A stepwise logistic regression (LR) model was generated to evaluate the association between contamination of groundwater by nitrates with several risk factors such as soil types, farm facilities and practises, and well characteristics. The odds ratio was calculated to estimate the degree of impact that the associated variables had on the risk of contamination in a semiconfined multilayer aquifer underlying rural areas of Buenos Aires, Argentina. Duplicate farm groundwater samples ( n = 160) were taken and nitrate was analyzed. Data, involving various farm factors, was gathered via two questionnaires concerning farm’s general and productive aspects, and well characteristics. Statistical tests were run between nitrates and each variable present in the survey. A 96.25% of the samples presented detectable nitrate levels, 40.91% of which had more than 45 ppm nitrates. The final LR model involved five of the variables under study: well age, soil permeability, depth of water table, location, and distance from well to contamination sources. Cross validation proved to be a good estimator of nitrate water contamination. Suspicions about how these characteristics influence groundwater contamination by nitrates were confirmed, and as these five factors represent a higher risk for this type of aquifer, their proper management may contribute to a better resource protection.

  14. Isolation of Rickettsia massiliae from Rhipicephalus sanguineus Ticks, Buenos Aires (Argentina).

    PubMed

    Cicuttin, G L; De Salvo, M N; La Rosa, I; Dohmen, F E Gury

    2015-12-01

    Rickettsia massiliae , a member of the spotted fever group of Rickettsia, was first isolated from a Rhipicephalus turanicus tick in France. In the New World, it has been detected in Rhipicephalus sanguineus ticks from different geographical locations in Argentina and the United States, but it has only been isolated in Arizona. The aim of this study was the isolation and genetic characterization of R. massiliae from R. sanguineus ticks collected from dogs in Buenos Aires city, Argentina. In total, 49 R. sanguineus ticks were collected from 10 dogs and grouped into 10 pools of 4-5 specimens. With a PCR assay, which detects a fragment of the Rickettsia genus-specific 23S-5S intergenic space, 1 pool of 5 ticks was found positive. Generated sequences exhibited 100% identity with R. massiliae . A new isolate, named CABA, was obtained from this pool by inoculating it into monolayers of Vero cells. Genotypic characteristics were determined, and results showed that fragments of the 23S-5S intergenic space, ompA, ompB, gltA, htrA, and sca1 genes had great similarity with R. massiliae strain Bar29 (Spain). Although few human cases have been confirmed for this pathogen, its circulation in urban areas is of great importance to public health. This isolation improves knowledge of the circulating pathogen and could improve future diagnostic processes as it allows the production of more specific antigens for serological testing.

  15. Ecotoxicological and genotoxic evaluation of Buenos Aires city (Argentina) hospital wastewater.

    PubMed

    Magdaleno, Anahí; Juárez, Angela Beatriz; Dragani, Valeria; Saenz, Magalí Elizabeth; Paz, Marta; Moretton, Juan

    2014-01-01

    Hospital wastewater (HWW) constitutes a potential risk to the ecosystems and human health due to the presence of toxic and genotoxic chemical compounds. In the present work we investigated toxicity and genotoxicity of wastewaters from the public hospital of Buenos Aires (Argentina). The effluent from the sewage treatment plant (STP) serving around 10 million inhabitants was also evaluated. The study was carried out between April and September 2012. Toxicity and genotoxicity assessment was performed using the green algae Pseudokirchneriella subcapitata and the Allium cepa test, respectively. Toxicity assay showed that 55% of the samples were toxic to the algae (%I of growth between 23.9 and 54.8). The A. cepa test showed that 40% of the samples were genotoxic. The analysis of chromosome aberrations (CA) and micronucleus (MN) showed no significant differences between days and significant differences between months. The sample from the STP was not genotoxic to A. cepa but toxic to the algae (%I = 41%), showing that sewage treatment was not totally effective. This study highlights the need for environmental control programs and the establishment of advanced and effective effluent treatment plants in the hospitals, which are merely dumping the wastewaters in the municipal sewerage system. PMID:25214834

  16. [Recent history: 12th International Conference on Cancer, Buenos Aires, Argentina, 1978].

    PubMed

    Spinelli, Hugo

    2014-04-01

    Using the approaches of history of the present, this article recovers the discussions surrounding the 12th International Conference on Cancer carried out in Buenos Aires in 1978, in reaction to which Georges Périès organized a "counter-conference" in Paris. In order to understand this discussion, the political situation of the time is described, as is the state of human rights at the time in Argentina, the role of the media - in particular the newspapers La Nación and Clarín and the magazine Gente - and the institutional position adopted by the National Academy of Medicine, as expressed in a letter sent to the presidents of the primary scientific societies of the world. The letter is reprinted in this text as a documentary source, taken from Memoria: Año 1978 (Presidencia de Dr. José E. Rivarola) [Acta: Year 1978 (Presidency of Dr. José E. Rivarola)]. The framework of the discussion makes reference to science's social policy versus science's supposed neutrality and the role of scientific societies.

  17. [Factors associated with academic success of medical students at Buenos Aires University].

    PubMed

    Borracci, Raúl A; Pittaluga, Roberto D; Álvarez Rodríguez, Juan E; Arribalzaga, Eduardo B; Poveda Camargo, Ricardo L; Couto, Juan L; Provenzano, Sergio L

    2014-01-01

    The aim of this study was to identify common factors relating to the academic success of medical students who were distinguished with honors at the Buenos Aires University. In 2011, 142 graduates were surveyed; the questionnaire included 59 questions on their sociodemographic environment, living conditions and social integration, motivation to study, learning capacity and health quality during their career. Compared to other students, these distinguished students more often lived in the city, far from their families; had been educated at private or universitary high schools, their economic needs were financed by their parents, who were on the whole professionals. Most of them were single and childless. The possibility of future employment oportunities (work) did not influence their choice of a medical career, academic success was important to them and they believed that success depended largely on personal effort; they knew how to handle anxiety, were sociable but independent and preferred solid experience to abstract conceptuality in order to obtain information. Our conclusion, within the current system of candidate selection, these results serve to calculate the covert self-selection mechanisms during the career, or in a more restrictive regime, to select those likely to reach academic success due to their privileged ambience. The analysis of demographic factors indicates some degree of inequality for socially disadvantaged students. Perhaps, a selection system based only on intellectual abilities would help identify and support the best candidates regardless of their social context. PMID:25555005

  18. [Transparency in public sector acquisitions. The case of hospitals in the City of Buenos Aires].

    PubMed

    Rossi, T; Murillo Fort, C; Puente Karolys, J C

    2002-01-01

    This paper deals with corruption and the lack of transparency in public sector purchases as well as with the main instruments to obtain adequate results in purchase negotiation.Firstly, we discuss how corruption causes concern to national governments, international organizations, academic centers, non-governmental organizations and society in general. The consequences of corruption in Argentina and other Latin American countries are highlighted, especially the effect of corruption on economic growth and the way it creates economic inefficiency and inequality.Secondly, the database created by the Subsecretary of Strategic Management of the Autonomous Government of the City of Buenos Aires is analyzed. The central purpose of this study is to evaluate the impact of the Administrative Reform of 1998 on the prices of 24 products acquired by 13 general acute care hospitals from 1998-1999. The weighted prices, the number of units purchased and the total number of contracts given in this period, as well as the products with the greatest utilization rate, are analyzed. Multivariante analysis was used to identify hospitals with appropriate activity and efficient budget administration (activity and negotiation indicators). Price development was analyzed using the regression technique (ordinary least squares), which demonstrated an 8% reduction in prices for the year 1999. The contribution of each hospital to this variation is presented using dummy variables. Thus, six of the 13 hospitals significantly contributed to the decrease in prices. Of these six, three hospitals also contributed to reduction in price dispersion. The results obtained allow us to conclude that, if public hospitals have adequate purchase negotiation instruments and a uniform legal framework, they can achieve a good level of activity. Furthermore, public hospitals can contribute to reductions in price and price dispersion, at the same time as improving efficiency in the assignation and utilization of

  19. Water quality-based toxics evaluation of Reconquista River, Province of Buenos Aires, Argentina

    SciTech Connect

    Herkovits, J.; Herkovits, F.D.; Perez-Coll, C.S.

    1995-12-31

    The water quality based toxics control is essential to evaluate the aggregate toxicity, bioavailability as well as for the detection and/or prediction of ecological impacts. Reconquista River valley is situated in the north area of Great Buenos Aires with a population of three million inhabitants. The river is loaded with industrial and municipal waste water. In the present preliminary study the authors report the toxicity found in surface water at a 6 sample stations (including a reference point and a stream) all of them downstream from mixing zone areas. The ecotoxicological study was performed with three native species (Bufo arenarum embryos, Cnesterodon decemmaculatus and a species of shrimp collected in an upstream reference site) during a 7 day renewal toxicity test conducted with 10 individuals (by duplicate) for each condition plus control. The results point out that the Bufo arenarum embryos test is the most sensitive to toxic substances as well as the better adapted species to the changing physico-chemical conditions of this river. The results obtained with embryos, expressed in Acute and Chronic Toxicity Units (according USEPA) range between <0.3--2 and <1--5 respectively (recommended magnitudes for industrial effluents according USEPA: 0.3 and 1 toxicity units respectively). Therefore, the toxicity found in Reconquista River ecosystem was up to 6 times higher than the maximal value recommended for industrial effluents. It is noteworthy that in the place where toxicity starts to rise, a large number of dead fishes were found and from that place downstream, no macroorganisms were found in the river. The results confirm the high sensitivity of Bufo arenarum embryos for continental waters ecotoxicological studies and the possibility of using this test as a short-term chronic toxicity method for water quality-based toxics control.

  20. [Sanitary conditions of community dining halls in greater Buenos Aires, Argentina].

    PubMed

    Arango, J; Agostini, A; Silvestre, A; Yaafar, M; López, C; Fishmann, H

    1997-10-01

    Because of the importance of food safety for public health, a study was done to determine the microbiologic quality of foods provided to patrons of community dining halls in greater Buenos Aires, Argentina. The study was descriptive and was conducted in three municipalities with intermediate or high poverty indices; a sample of 52 dining halls was selected randomly from those municipalities. Information was collected from April 1994 through April 1995 by means of surveys, direct observations, and microbiologic analyses of the water and food served. Water and food analysis followed the recommendations of the Argentine Food Code and the International Commission on Microbiologic Specifications for Foods, respectively. The results were interpreted according to the values for cooked foods agreed upon by the Working Group on Food Monitoring. The microbiologic analysis revealed that 28 (54%) of the dining halls had food or water of inadequate quality and 11 (21%) had inadequacies in both. Bacillus cereus was the most commonly found pathogenic bacterium in the food samples analyzed. The factors that were significantly associated with unsatisfactory microbiologic conditions in the food and water were lack of disinfection of the water (P = 0.009), lack of hygiene in the environment (P = 0.03), the presence of rodents and insects (P = 0.05), the supply system for raw materials (P = 0.01), and a relatively small number of users, i.e., fewer than 100 (P = 0.0008). It was concluded that water disinfection and general clean-up of the dining halls were measures that could be put in place by providing education in the areas of hygiene and food handling to supervisors and employees. The results obtained also indicate the need to include a food safety evaluation among the general evaluation criteria for food assistance programs. PMID:9445768

  1. Pleistocene and holocene beaches and estuaries along the Southern Barrier of Buenos Aires, Argentina

    NASA Astrophysics Data System (ADS)

    Isla, Federico I.; Cortizo, Luis C.; Schnack, Enrique J.

    The Buenos Aires aggradation plain has a good record of Quaternary sea-level fluctuations. To the east of the Tandilia Range, the elevations of the Pleistocene beaches respond to the tectonic behaviour of the Salado Basin. Holocene beaches indicate a maximum transgression higher than 2 m. The low relief permitted an extended horizontal record of beach/chenier plains interfingered with estuarine environments (coastal lagoons, marshes) covered by a sandy (Eastern) barrier. Between the Tandilia and Ventania ranges, the location of Pleistocene and Holocene beaches are related to a former higher relief; i.e. they are attached to low-altitude cliffs and underneath cliff-top dunes composing the Southern Barrier. At Claromecó, Pleistocene gravel beaches, mostly composed of caliche pebbles, occur at heights between 4 and 7 m, and are overlying estuarine Pleistocene environments. Beaches of the same age are at a level of 10 m at Mar del Plata Harbour and Arroyo Sotelo (west of Mar Chiquita Lagoon). Holocene beaches found at Punta Mogotes and Costa Bonita are at higher altitudes than on the Eastern Barrier (ca. 2-4 m). The Holocene estuarine sequences are related laterally to present operating inlets (Las Brusquitas, La Ballenera, Quequén Grande, Claromecó, Quequén Salado). They are seldon thicker than 2.4 m, and comprise basal layers of black muds; towards the top, the layers are thinner, of coarser grain size and white colours. Grain-size analyses were performed comparatively on Pleistocene, mid-Holocene and present beaches. Sangamonian beaches aregravelly or coarser than medium sand (mean). Holocene beaches are usually coarser than medium sand, but dominantly shelly to the north of Mar del Plata, and composed of volcanic clasts to the south of this city. Modern beaches are dominated by fine sand, except at some erosive beaches between the Mar del Plata capes. They have a lesser content of shells than those of mid-Holocene.

  2. Magnetic Characterization of Stream-Sediments From Buenos Aires Province, Argentina, Affected by Pollution

    NASA Astrophysics Data System (ADS)

    Chaparro, M. A.; Sinito, A. M.; Bidegain, J. C.; Gogorza, C. S.; Jurado, S.

    2001-12-01

    A wide urban area from Northeast of Buenos Aires Province is exposed to an important anthropogenic influence, mainly due to industrial activity. In this two water streams were chosen: one of them (Del Gato stream, G) next to La Plata City and the another one (El Pescado stream, P) on the outskirts of the city. Both streams have similar characteristics, although the first one (G) has a higher input of pollutants (fluvial effluents, fly ashes, solid wastes, etc.) than the last one (P). Sediments analyzed in this work are limes from continental origin of PostPampeano (Holocene). Although, some cores were affected by sandy-limy sediments with mollusc valves from Querandino Sea (Pleistocene - later Holocene) and limy sediments of chestnut color with calcareous concretions from the Ensenadense. Magnetic measurements and geochemical studies were carried out on the samples. Among the magnetic parameters, specific susceptibility (X), X frequency-dependence (Xfd%), X temperature-dependence, Natural Remanent Magnetization (NRM), Isothermal Remanent Magnetization (IRM), Saturation IRM (SIRM), coercivity of remanence (Bcr), S ratio and SIRM/X ratio, Anhysteric Remanent Magnetization (ARM), Magnetic and Thermal Demagnetization were studied. The magnetic characteristics for both sites indicate the predominance of magnetically soft minerals on G site and relatively hard minerals on P site. Magnetite is the main magnetic carrier, Pseudo Single Domain and Single Domain grains were found. Chemical studies show (in some cases) a high concentration for some heavy metals (Pb, Cu, Zn, Ni and Fe) on the upper 22-cm. Contents of heavy metals and ARM were correlated. Very good correlation (R> 0.81) is found for Cu, Zn, Ni, Fe and the sum (of Pb, Cu, Zn and Ni), and a weaker correlation for Pb.

  3. Argentina: risk factors and maternal mortality in La Matanza, Province of Buenos Aires, 1990.

    PubMed

    Szmoisz, S; Vuegen, S E; Plaza, A S; Barracchini, R; Checa, S; Derlindati, A; Espinola, D A; Rúgolo, E C

    1995-01-01

    An evaluation of the health services infrastructure of the La Matanza part of Buenos Aires in 1990 was carried out in addition to an evaluation of maternal mortality case studies. This procedure allowed for an assessment of factors related to the performance of health services and the health behaviour of women which, concomitantly, led to maternal deaths. Approximately 50% of maternal deaths went unreported in La Matanza on the basis of record checks performed in the institutions, hence the maternal mortality was twice as high as officially indicated for 1990. Flaws in the proper clinical diagnosis of the causes of deaths were detected and a higher degree of precision was called for. In the case of women who came from the poorest section of La Matanza, most deaths were due to complications related to abortion (either self-induced or non-professionally induced). Most of the maternal deaths could have been avoided. The sociological enquiry revealed conflicting social pressures which led the women onto the path of maternal death. The men were found not to be involved in the health issues arising from pregnancy and delivery, and the reproductive process was seen to lie exclusively in the women's domain. The services were not prepared to cater for the needs of poor women, and the inadequacy of the existing system to reach the women in need was well documented. Detection of women at risk was lacking in most establishments and, with the exception of one hospital, referral procedures did not exist. At the municipal level the absence of a policy for maternal and child health was noted.(ABSTRACT TRUNCATED AT 250 WORDS)

  4. [Transparency in public sector acquisitions. The case of hospitals in the City of Buenos Aires].

    PubMed

    Rossi, T; Murillo Fort, C; Puente Karolys, J C

    2002-01-01

    This paper deals with corruption and the lack of transparency in public sector purchases as well as with the main instruments to obtain adequate results in purchase negotiation.Firstly, we discuss how corruption causes concern to national governments, international organizations, academic centers, non-governmental organizations and society in general. The consequences of corruption in Argentina and other Latin American countries are highlighted, especially the effect of corruption on economic growth and the way it creates economic inefficiency and inequality.Secondly, the database created by the Subsecretary of Strategic Management of the Autonomous Government of the City of Buenos Aires is analyzed. The central purpose of this study is to evaluate the impact of the Administrative Reform of 1998 on the prices of 24 products acquired by 13 general acute care hospitals from 1998-1999. The weighted prices, the number of units purchased and the total number of contracts given in this period, as well as the products with the greatest utilization rate, are analyzed. Multivariante analysis was used to identify hospitals with appropriate activity and efficient budget administration (activity and negotiation indicators). Price development was analyzed using the regression technique (ordinary least squares), which demonstrated an 8% reduction in prices for the year 1999. The contribution of each hospital to this variation is presented using dummy variables. Thus, six of the 13 hospitals significantly contributed to the decrease in prices. Of these six, three hospitals also contributed to reduction in price dispersion. The results obtained allow us to conclude that, if public hospitals have adequate purchase negotiation instruments and a uniform legal framework, they can achieve a good level of activity. Furthermore, public hospitals can contribute to reductions in price and price dispersion, at the same time as improving efficiency in the assignation and utilization of

  5. [Scientific research in public health institutions of the City of Buenos Aires].

    PubMed

    Ferrero, Fernando; Ossorio, María Fabiana; Eiguch, Kumiko

    2013-01-01

    Hospitals with academic activities offer better services to their patients. The Government of Buenos Aires City (GBAC) has an important network of health facilities, but there is little information regarding their academic activities. Our aim was to estimate the proportion of articles included in MEDLINE originated in GBAC hospitals, to evaluate their contribution regarding the whole country (Argentina), and to describe some characteristics of these papers. It is a cross-sectional study based on a MEDLINE search, using the name of each of the 33 GBAC hospitals in the "affiliation" field. Among 22 million references, 1781 belonged to GBAC hospitals (4.4% of all references from Argentina); 66.5% were in English, 83.7% were related to humans, 30.1% were from the last 5 years, 8.3% included high level scientific evidence research (meta-analysis or randomized controlled trials), and 31.1% included research in children. Regarding the rest of the references from Argentina, those from GBAC hospitals included less papers in English (66.5% vs. 90.0%; OR: 0.22; p < 0.0001), less recent papers (30.1% vs. 34.6%; OR: 0.81; p < 0.0001), more research in humans (83.7% vs. 37.3%; OR: 8.65; p < 0.0001), more randomized controlled trials (8.0% vs. 2.8%; OR: 2,9; p < 0.0001), and more research in children (31.1% vs. 9.2%; OR: 4.44; p < 0.0001). GBAC hospitals contributed with 4.4% of references attributed to Argentina in MEDLINE. References from GBAC hospitals were less recent, and included more randomized controlled trials and research in children.

  6. HIV-1, HIV-2, HTLV-I/II and STD among female prostitutes in Buenos Aires, Argentina.

    PubMed

    Zapiola, I; Salomone, S; Alvarez, A; Scolastico, M C; Koessel, R A; Lemus, J; Wainstein, C; Muchinik, G

    1996-02-01

    To determine the prevalence of HIV-1 and HTLV-I/II among female prostitutes from different areas of the city of Buenos Aires, we studied serum samples from 237 individuals (mean age: 25; range 17 to 39). Prostitutes were recruited from 16 different Buenos Aires locations with different economical status. Information on sexual behaviour, health and socioeconomic conditions was collected through a questionnaire. HIV-1 and HTLV-I/II antibodies (ab) were tested by ELISA (Abbott) and Particle agglutination (Fujirebio, Tokyo) respectively. Positive results were confirmed by immunofluorescence assay. Samples that were positive for HIV-1 antibodies were also tested for p24 antigen (Abbott). VDRL for syphilis was performed in all samples. Fifteen (6.3%) out of the 237 individuals were positive for HIV-1 antibodies. Moreover, 2 (0.8%) HIV-1 seropositive prostitutes were also positive for HTLV-I/II antibodies and for HIV p24-Ag. Even though PCR for HTLV-I/II was not performed, titration by IFA in these two samples suggests HTLV-I. Our serologic results indicate a relatively high HIV-1 infection among prostitutes working in Buenos Aires. As we previously mentioned for other risk groups, we found an association between HTLV-I/II and HIV-1 infection in this particular group. Although we did not find any significant difference between HIV-1 seropositivity and the variables analyzed through the questionnaire, the prevalence of HIV-1 infection was higher in prostitutes working in mask brothels ('sauna or massage houses') as compared with hotel or street prostitutes.

  7. 210Pb dating of sediments in a heavily contaminated drainage channel to the La Plata estuary in Buenos Aires, Argentina.

    PubMed

    Di Gregorio, D E; Fernández Niello, J O; Huck, H; Somacal, H; Curutchet, G

    2007-01-01

    Concentrations of (210)Pb and (137)Cs in sediment samples collected from two cores at a drainage channel to the La Plata river estuary in Buenos Aires, Argentina, were measured using ultralow-background detection systems. The (210)Pb data were used to determine the rate of sediment accumulation of the sites. These results were correlated with some heavy metal (chromium and lead) concentrations of the samples in an attempt to characterize the historical input of contaminants due to the industrial development, which has taken place in this area over the last century. The (137)Cs measurements demonstrate that cesium dating is not adequate in regions of the southern hemisphere.

  8. Geochemical Assessment of Groundwater in the Peri-urban Environment of Buenos Aires, Argentina

    NASA Astrophysics Data System (ADS)

    Gallardo, A.

    2014-12-01

    Groundwater pollution is a major concern in peri-urban environments. Thus, water quality is being investigated at several domestic wells in Brandsen, 70 km south of Buenos Aires, Argentina. To present, about 20 water sources were sampled in orchards and small farms of the area. There is limited data about the wells construction, although collected information suggests that groundwater is derived from the superficial sandy loams of the Pampean Aquifer. Samples were analysed for major inorganic elements using ion chromatography and ICP-MS. Titration was used to estimate alkalinity. Physical characteristics (EC, pH, temperature) were measured on site. Results show that groundwater pH ranges from 6.5 to 7.8, with a specific conductance of 180 to 255 mS/m. A peak of 360 mS/m in one horticultural parcel is associated to local NO3- concentrations up to 140 mg/L. This value exceeds the maximum recommendations set by the WHO (50 mg/L). Considering that fertilizer inputs in that property are negligible, the high levels of NO3- might be attributed to effluents from a neighbour septic tank. An increase in NO3- (>150mg/L) was also detected in two conventional farms. This increase correlates to elevated SO42- concentrations (>300 mg/L) suggesting thus, fertilizers percolation into the saturated zone. The leaching of these fluids might be exacerbated by irrigation during new planting, and accumulations of fertilizer-solids in the root zones from previous seasons. Chloride concentrations average ~90 mg/L and would not pose a threat to health at the moment. Its main origin would be related to connate waters in the loam matrix, although some anthropogenic inputs might occur in the previously described farms. In general, the rest of the analysed elements fall within acceptable levels for drinking purposes as well. Nevertheless, further work is still necessary to better define the fate of the potential harmful elements and assess seasonal variations in water quality.

  9. [Distribution of findings of scorpions in Buenos Aires city in the period 2001-2012 and their sanitary implications].

    PubMed

    Blanco, Guillermo; Laskowicz, Rodrigo D; Lanari, Laura C; Scarlato, Eduardo; Damin, Carlos; de Titto, Ernesto H; de Roodt, Adolfo R

    2016-02-01

    Scorpion stings and their associated mortality increased in the last years in Argentina, with a cumulative record of 73,617 cases and 30 deaths during the period 2001-2012, occurring almost all the deaths in pediatric patients. However, deaths due to severe envenoming by scorpion stings have not been recorded in Buenos Aires city and suburban regions, although the presence of scorpions in this city has been increasingly reported. We studied the temporal and geographical distribution of Tityus trivittatus findings in Buenos Aires city from the database of the Research and Development Area from the National Institute for Production of Biologics of the National Ministry of Health during the period 10/01/2001 to 31/12/2012 in order to correlate these findings with the distribution of health centers in the city. In this period 385 consults with identification of scorpions were recorded. Annual records showed a growing trend. Georeferenced data showed that findings appeared to increase in the surroundings of metro and train stations, mainly at the east of the city with expansion to the west. Although Toxicology services are geographically related to the zones with higher density of finding of scorpions, the accessibility to the centers with antivenom may hinder its application in the recommended time; some measures to avoid possible delays in the application of the treatment are suggested. PMID:26914081

  10. [Distribution of findings of scorpions in Buenos Aires city in the period 2001-2012 and their sanitary implications].

    PubMed

    Blanco, Guillermo; Laskowicz, Rodrigo D; Lanari, Laura C; Scarlato, Eduardo; Damin, Carlos; de Titto, Ernesto H; de Roodt, Adolfo R

    2016-02-01

    Scorpion stings and their associated mortality increased in the last years in Argentina, with a cumulative record of 73,617 cases and 30 deaths during the period 2001-2012, occurring almost all the deaths in pediatric patients. However, deaths due to severe envenoming by scorpion stings have not been recorded in Buenos Aires city and suburban regions, although the presence of scorpions in this city has been increasingly reported. We studied the temporal and geographical distribution of Tityus trivittatus findings in Buenos Aires city from the database of the Research and Development Area from the National Institute for Production of Biologics of the National Ministry of Health during the period 10/01/2001 to 31/12/2012 in order to correlate these findings with the distribution of health centers in the city. In this period 385 consults with identification of scorpions were recorded. Annual records showed a growing trend. Georeferenced data showed that findings appeared to increase in the surroundings of metro and train stations, mainly at the east of the city with expansion to the west. Although Toxicology services are geographically related to the zones with higher density of finding of scorpions, the accessibility to the centers with antivenom may hinder its application in the recommended time; some measures to avoid possible delays in the application of the treatment are suggested.

  11. [Food environment and space accessibility evaluation to perform physical activity in 3 socially contrasting neighbourhoods of Buenos Aires city].

    PubMed

    Garipe, Leila Yasmin; Gónzalez, Verónica; Biasizzo, Antonella; Soriano, Jennifer Laila; Perman, Gaston; Giunta, Diego

    2014-01-01

    Due to the environmental influences on health, the goal of this study was to describe and compare the built environment in 3 socially contrasting neighbourhoods of Buenos Aires city.In 2011 a cross-sectional study was conducted in 3 socially contrasting neighbourhoods of Buenos Aires city: Recoleta (upper class), Almagro (middle class) and Constitución (lower class). Grocery stores and food stands were surveyed as well as all suitable spaces to perform physical activity. An analysis was conducted to assess the density of every food outlet per Km2 of each neighbourhood's area and per 10000 inhabitants. 2778 food stores and 149 outdoor physical activity facilities were surveyed. A higher density was observed in Constitución for fast food restaurants (Recoleta 3.6; Almagro 2.4; Constitución 6.7) and food stands (Recoleta 4.2; Almagro 1.2; Constitución 25.7) and a lower density for outdoor physical activity facilities. Population density and area density proved to be analogous. Statistically relevant differences were observed regarding the dimension of each food outlet: grocery stores, fruit stands, pubs, restaurants and food stands, as well as in the number of food stores and outdoor physical activity facilities. The information gathered in this study could be highly useful for public health policies on healthy lifestyles, and could eventually redefine the built environment in order to improve the city's equality regarding outdoor physical activity facilities and food stores. PMID:25647550

  12. [Food environment and space accessibility evaluation to perform physical activity in 3 socially contrasting neighbourhoods of Buenos Aires city].

    PubMed

    Garipe, Leila Yasmin; Gónzalez, Verónica; Biasizzo, Antonella; Soriano, Jennifer Laila; Perman, Gaston; Giunta, Diego

    2014-01-01

    Due to the environmental influences on health, the goal of this study was to describe and compare the built environment in 3 socially contrasting neighbourhoods of Buenos Aires city.In 2011 a cross-sectional study was conducted in 3 socially contrasting neighbourhoods of Buenos Aires city: Recoleta (upper class), Almagro (middle class) and Constitución (lower class). Grocery stores and food stands were surveyed as well as all suitable spaces to perform physical activity. An analysis was conducted to assess the density of every food outlet per Km2 of each neighbourhood's area and per 10000 inhabitants. 2778 food stores and 149 outdoor physical activity facilities were surveyed. A higher density was observed in Constitución for fast food restaurants (Recoleta 3.6; Almagro 2.4; Constitución 6.7) and food stands (Recoleta 4.2; Almagro 1.2; Constitución 25.7) and a lower density for outdoor physical activity facilities. Population density and area density proved to be analogous. Statistically relevant differences were observed regarding the dimension of each food outlet: grocery stores, fruit stands, pubs, restaurants and food stands, as well as in the number of food stores and outdoor physical activity facilities. The information gathered in this study could be highly useful for public health policies on healthy lifestyles, and could eventually redefine the built environment in order to improve the city's equality regarding outdoor physical activity facilities and food stores.

  13. Late Quaternary continental and marine sediments of northeastern Buenos Aires province (Argentina): Fossil content and paleoenvironmental interpretation

    NASA Astrophysics Data System (ADS)

    Fucks, Enrique; Aguirre, Marina; Deschamps, Cecilia M.

    2005-10-01

    Abundant invertebrate and vertebrate fossil remains that exhibit excellent preservation and were collected from deposits of both continental and marine origins at Pilar (Buenos Aires, Argentina) add paleoenvironmental data from the northeastern Buenos Aires province area linked to sea-level oscillations and climate variability since approximately 120 ka BP (marine oxygen isotope stage [MOIS] 5e). Two new fossiliferous localities discovered in the Luján River Valley allow for detailed geological studies and new dating of molluscan shells and bones. The studies suggest salinity changes during the Last Interglacial (8 m above m.s.l., min. 14C>40 ka) and the mid-Holocene transgression (5 m above m.s.l., 7-3 14C ka BP) compared with the modern pattern along the adjacent littoral (Río de la Plata). The marine sequences represent the innermost boundary of the sea-level transgression in that area and contain a biogenic record (bivalves, gastropods, forams, ostracods) that indicates marginal marine environments (higher salinity than at present). Vertebrates and molluscs from the continental sequence suggest a freshwater habitat in which remains of marine fish must be allochthonous, probably incorporated by postmortem fluvial transport to the final depositional environment.

  14. Histopathology associated with angiostrongylosis in Akodon species (Rodentia: Sigmodontinae) from Sierra de la Ventana, Buenos Aires, Argentina.

    PubMed

    Robles, María del Rosario; Perfumo, Carlos; Kinsella, John M; Navone, Graciela T

    2012-12-01

    Angiostrongylus is a metastrongylid nematode genus that includes species found in carnivores, rodents, and occasionally primates (including humans). About 100 species of wild sigmodontine rodents occur in Argentina, 16 of which are species of Akodon. The lungs of Akodon azarae and Akodon dolores from Sierra de la Ventana, Buenos Aires, Argentina, were examined, and 2 of 10 A. dolores specimens were positive for angiostrongylosis, showing macroscopic lesions of verminous pneumonia. Adults found were identified as Angiostrongylus morerai. Histopathology of the lungs revealed multiple nodules in the interstitium, alveoli, and vessels, resulting in interstitial fibrosis and the destruction of small capillaries and arterioles. Since extensive pathology in the lung was noted here from only 2 adults, it is probable that heavier infections may cause mortality in their hosts. This is the first record of this nematode species from A. dolores , expanding its geographic distribution to the southwest of Buenos Aires Province, and the first description of the histopathology of larval angiostrongylosis in a wild rodent from South America.

  15. Long-term landscape development: a perspective from the southern Buenos Aires ranges of east central Argentina

    NASA Astrophysics Data System (ADS)

    Demoulin, A.; Zarate, M.; Rabassa, J.

    2005-06-01

    Traditionally, the long-term landscape evolution of the southern Buenos Aires ranges of east central Argentina has been related to the influence of the Andean orogeny. We describe the large-scale morphological units and associated weathering products in the Tandilia and Ventania ranges. Two main planation surfaces are encountered at varying altitudes in different sectors of these ranges. The lower surface is characterized by roots of kaolinized weathering profiles in the Tandil area and silicified conglomerates around Sierra de La Ventana. In an interpretative model linking the range morphogenesis to the tectonosedimentary evolution of the bordering Salado and Colorado Basins, we suggest that the main morphogenetic stages are related to the late Jurassic-early Cretaceous south Atlantic rifting and Miocene tectonic reactivation induced by the Andean orogeny. Thus, the uplifted surfaces appear much older than commonly believed: pre-Cretaceous and Paleogene. Although they contradict recent results of apatite fission-track studies along the South America and South Africa passive margins, the implied low denudation rates (˜4 m/My) can be explained by the limited Meso-Cenozoic uplift suffered by the southern Buenos Aires ranges. The discussion also shows the limits of the comparison that can be made with the South African planation surfaces.

  16. Comparison of vitamin A intake from breast milk and from complementary foods in the diet of six-month old infants in Jujuy and Buenos Aires.

    PubMed

    Greco, Carola Beatriz; López, Laura Beatriz; Rodríguez, Viviana; Dyner, Luis; Gibson, Verónica; Pinotti, Luisa Virginia; Ronayne de Ferrer, Patricia Ana

    2014-10-01

    The objective of this study was to compare the dietary pattern of two socio-culturally different populations, focusing on the vitamin A intake from breast milk and from the most commonly consumed complementary foods. Dietary surveys on six-month old infant feeding were administered to breastfeeding mothers in Jujuy (n= 44) and Buenos Aires (n= 95).For milk, the level of retinol was determined by liquid chromatography; for foods, calorie and vitamin A values were estimated. In Jujuy and Buenos Aires, 75.6% and 64.5% of infants were receiving complementary foods at six months old, and more than 50% had started earlier. Milk retinol ranged from 0.02 to 1.19 μg/ mL and from 0.09 to 1.94 μg/mL in mothers in Jujuy and Buenos Aires, respectively.Values <0.30 μg/mL, indicative of vitamin A deficiency, were observed in 67.4% and 26.1% of milk samples, respectively. Usually consumed foods may provide sufficient vitamin A for Buenos Aires participants, but insufficient for those in Jujuy.

  17. Talking to Strangers--A Sociolinguistic Experiment: Variation in Initial Dyadic Interactions between Spanish-Speakers in Early 21st Century Buenos Aires, Argentina

    ERIC Educational Resources Information Center

    Dziugis, Mary Ann

    2010-01-01

    What are the chances of a dyad of Spanish-speaking strangers using informal address in casual, initial interactions in Buenos Aires, Argentina, today? To discover the pattern(s) of contemporary address, the Principal Investigator (PI) conducted a sociolinguistic experiment focusing on strangers' initial interactions to minimize the influence of…

  18. Prosodic Transfer in Learner and Contact Varieties: Speech Rhythm and Intonation of Buenos Aires Spanish and L2 Castilian Spanish Produced by Italian Native Speakers

    ERIC Educational Resources Information Center

    Gabriel, Christoph; Kireva, Elena

    2014-01-01

    A remarkable example of Spanish-Italian contact is the Spanish variety spoken in Buenos Aires (Porteño), which is said to be prosodically "Italianized" due to migration-induced contact. The change in Porteño prosody has been interpreted as a result of transfer from the first language (L1) that occurred when Italian immigrants learned…

  19. "Quien Sabe Mas Lucha Mejor": Adult Educators' Care of the Self Practices within Social Movements in Buenos Aires, Argentina

    ERIC Educational Resources Information Center

    O'Donnell, Jennifer Lee

    2014-01-01

    This article looks at popular adult educators' care of the self practices within social movements in Buenos Aires, Argentina. It answers the following questions: How is popular adult education practiced amongst educators in social movements? What can studying popular adult educators' care of the self practices offer the field of adult…

  20. The Moral Dimension of Class and Gender Identity-Making: Poverty and Aggression in a Secondary School in the City of Buenos Aires

    ERIC Educational Resources Information Center

    Meo, Analia Ines

    2011-01-01

    This paper presents some results of a qualitative study carried out in a secondary school in the city of Buenos Aires (Argentina). It examines how two students from poor families responded to, and viewed, aggression by peers at their school. This paper argues that the examination of students' narratives about aggression (based on classism and…

  1. Reading for All; Proceedings of the IRA (International Reading Association) World Congress on Reading (4th, Buenos Aires, Argentina, August 3-5, 1972).

    ERIC Educational Resources Information Center

    Karlin, Robert, Ed.

    This book contains papers presented at the Fourth International Reading Association World Congress on Reading in Buenos Aires, Argentina, in August 1972. The contents of the book are divided into three parts: "Literacy and Literature" includes papers on libraries, books, and reading by Jorge Borges, the future of reading by Theodore Harris, the…

  2. Insights into Pleistocene palaeoenvironments and biostratigraphy in southern Buenos Aires province (Argentina) from continental deposits

    NASA Astrophysics Data System (ADS)

    Beilinson, E.; Gasparini, G. M.; Soibelzon, L. H.; Soibelzon, E.

    2015-07-01

    The coastal cliffs of the Buenos Aires province (Argentina) have been the subject of intense paleontological studies since the XIX century. Therefore, many of the type localities in which is based the late Cenozoic Pampean biostratigraphic/chronostratigraphic scheme are located in this area. In this context, the sedimentites that crop out near the mouth of the Chocorí Creek contain a set of palaeontological sites that, because of their richness and well-preserved fossil content, hold high national and international importance. The aims of the present contribution are: 1) to make a stratigraphic and sedimentological characterization of the study area; 2) to list the fauna outcropped at these palaeontological sites and establish a biostratigraphic framework; 3) to elaborate a palaeoenvironmental model for the area. The study interval was informally subdivided into a lower, middle and upper interval. Interpretation was based on the presence of a number of key features such as architectural elements; channel:overbank ratio and palaeosol occurrence. The first two intervals were interpreted as continental deposits of a fluvio-alluvial nature and are the focus of this paper. The upper interval was related to foreshore marine deposits and will be studied in a future contribution. The lower interval is characterized mainly by overbank architectural elements in which calcisols and argillic protosols were identified. Channel-fill deposits are isolated and surrounded by fine-grained overbank successions and sedimentary structures are suggestive of mixed-load transport. The contact between the lower and middle intervals is an irregular, highly erosive surface characterized by a significant vertical change in the facies. This surface defines the base of multistorey sandbodies which's internal arrangement alongside with the low participation of overbank deposits suggests deposition by a braided fluvial system. Palaeosols and vertebrate fossils were used as palaeoclimatic

  3. Presentación del estudio "Links" de hombres que tienes sexo con hombres en Buenos Aires, Argentina.

    PubMed

    Carballo-Diéguez, Alex; Avila, María M; Balán, Iván C; Marone, Rubén; Pando, María A; Barreda, Victoria

    2011-03-01

    Estudios previos en Buenos Aires reportaron altas prevalencias de HIV entre HSH, con valores que oscilan entre 9 y 14% durante casi 10 años de continuo testeo. El objetivo principal de este estudio fue la evaluación de factores relacionados al comportamiento de alto riesgo para transmisión del HIV entre HSH entre los que se incluyen el conocimiento y factores emocionales, socioculturales y ambientales. Por otro lado se realizó la estimación de prevalencia e incidencia de HIV utilizando RDS (Respondent Driven Sampling), así como la presencia de otras infecciones de transmisión sexual. Por último se evaluaron los hábitos de testeo para HIV indagando que factores facilitan o impiden su realización. El estudio constó de dos fases, en primer lugar una fase cualitativa y posteriormente una fase cuantitativa con una duración total de 4 años y medio. Durante la fase cualitativa se realizaron 44 entrevistas individuales en profundidad, 8 grupos focales y 10 observaciones etnográficas (hoteles, baños públicos ("teteras"), cines pornográficos, fiestas privadas, dark rooms y discotecas). Durante la fase cuantitativa del estudio se realizó el reclutamiento de 500 participantes que provinieron de la Ciudad Autónoma de Buenos Aires, así como del Gran Buenos Aires. El reclutamiento se comenzó con 16 participantes llamados semillas. Se realizó el diagnóstico de infección por HIV, hepatitis B y C (HBV y HCV), Treponema pallidum, Virus Papiloma Humano (HPV) y Chlamidias. La colaboración establecida entre los grupos de trabajo enfocados en áreas diversas posibilitó el abordaje conjunto de nuevas estrategias de investigación antes no exploradas en nuestro país. Los resultados más relevantes de esta investigación serán progresivamente publicados en sucesivos números de Actualizaciones en SIDA.

  4. Presentación del estudio “Links” de hombres que tienes sexo con hombres en Buenos Aires, Argentina

    PubMed Central

    Carballo-Diéguez, Alex; Ávila, María M; Balán, Iván C.; Marone, Rubén; Pando, María A.; Barreda, Victoria

    2011-01-01

    Resumen Estudios previos en Buenos Aires reportaron altas prevalencias de HIV entre HSH, con valores que oscilan entre 9 y 14% durante casi 10 años de continuo testeo. El objetivo principal de este estudio fue la evaluación de factores relacionados al comportamiento de alto riesgo para transmisión del HIV entre HSH entre los que se incluyen el conocimiento y factores emocionales, socioculturales y ambientales. Por otro lado se realizó la estimación de prevalencia e incidencia de HIV utilizando RDS (Respondent Driven Sampling), así como la presencia de otras infecciones de transmisión sexual. Por último se evaluaron los hábitos de testeo para HIV indagando que factores facilitan o impiden su realización. El estudio constó de dos fases, en primer lugar una fase cualitativa y posteriormente una fase cuantitativa con una duración total de 4 años y medio. Durante la fase cualitativa se realizaron 44 entrevistas individuales en profundidad, 8 grupos focales y 10 observaciones etnográficas (hoteles, baños públicos (“teteras”), cines pornográficos, fiestas privadas, dark rooms y discotecas). Durante la fase cuantitativa del estudio se realizó el reclutamiento de 500 participantes que provinieron de la Ciudad Autónoma de Buenos Aires, así como del Gran Buenos Aires. El reclutamiento se comenzó con 16 participantes llamados semillas. Se realizó el diagnóstico de infección por HIV, hepatitis B y C (HBV y HCV), Treponema pallidum, Virus Papiloma Humano (HPV) y Chlamidias. La colaboración establecida entre los grupos de trabajo enfocados en áreas diversas posibilitó el abordaje conjunto de nuevas estrategias de investigación antes no exploradas en nuestro país. Los resultados más relevantes de esta investigación serán progresivamente publicados en sucesivos números de Actualizaciones en SIDA. PMID:25264397

  5. Presentación del estudio "Links" de hombres que tienes sexo con hombres en Buenos Aires, Argentina.

    PubMed

    Carballo-Diéguez, Alex; Avila, María M; Balán, Iván C; Marone, Rubén; Pando, María A; Barreda, Victoria

    2011-03-01

    Estudios previos en Buenos Aires reportaron altas prevalencias de HIV entre HSH, con valores que oscilan entre 9 y 14% durante casi 10 años de continuo testeo. El objetivo principal de este estudio fue la evaluación de factores relacionados al comportamiento de alto riesgo para transmisión del HIV entre HSH entre los que se incluyen el conocimiento y factores emocionales, socioculturales y ambientales. Por otro lado se realizó la estimación de prevalencia e incidencia de HIV utilizando RDS (Respondent Driven Sampling), así como la presencia de otras infecciones de transmisión sexual. Por último se evaluaron los hábitos de testeo para HIV indagando que factores facilitan o impiden su realización. El estudio constó de dos fases, en primer lugar una fase cualitativa y posteriormente una fase cuantitativa con una duración total de 4 años y medio. Durante la fase cualitativa se realizaron 44 entrevistas individuales en profundidad, 8 grupos focales y 10 observaciones etnográficas (hoteles, baños públicos ("teteras"), cines pornográficos, fiestas privadas, dark rooms y discotecas). Durante la fase cuantitativa del estudio se realizó el reclutamiento de 500 participantes que provinieron de la Ciudad Autónoma de Buenos Aires, así como del Gran Buenos Aires. El reclutamiento se comenzó con 16 participantes llamados semillas. Se realizó el diagnóstico de infección por HIV, hepatitis B y C (HBV y HCV), Treponema pallidum, Virus Papiloma Humano (HPV) y Chlamidias. La colaboración establecida entre los grupos de trabajo enfocados en áreas diversas posibilitó el abordaje conjunto de nuevas estrategias de investigación antes no exploradas en nuestro país. Los resultados más relevantes de esta investigación serán progresivamente publicados en sucesivos números de Actualizaciones en SIDA. PMID:25264397

  6. [From educational and health tourism for children to social tourism: vacation camps in the Province of Buenos Aires, Argentina].

    PubMed

    Bruno, Perla

    2015-12-01

    The early twentieth century saw the rise of vacation camps for frail children as educational and health-giving experiences provided by medical and philanthropic organizations. This article analyzes some of these early experiences, seen here as the predecessors of social tourism, in the Province of Buenos Aires. A combination of written sources are examined, mainly institutional reports, periodicals such as the Monitor de la Educación Común - published by the Consejo Nacional de Educación (National Board of Education) - or laws, with photographs and plans for different examples. I argue that these buildings were both physical and cultural "brands" in the places where they were located, and that their architectural structure encapsulated ideas about leisure space and cures in unique natural environments.

  7. Highlights of the ecancer/SAC First International Prostate Cancer Symposium, 11–12 March 2016, Buenos Aires, Argentina

    PubMed Central

    Villalba, Marcelo Blanco; Bramajo, Marina; Bruno, Mario

    2016-01-01

    The ecancer/SAC First International Prostate Cancer Symposium, held in Buenos Aires, included national, regional, and international experts in the field of prostate cancer. More than 200 professionals from a variety of areas (clinical urologists, pathologists, oncologists, biologists, imaging specialists, radiation therapists, and generalist doctors, among others) attended, and they proposed multidisciplinary management of prostate pathology from the start in concordance with the ideas set forth by the organising committee. A radiotherapy workshop was also held during the symposium, in which new techniques and their possible uses were specifically discussed. In addition to the local doctors, Dr Lilian Faroni (COI Group, Rio de Janeiro, Brazil), Dr Leonardo Carmona (Chilean Head and Neck Institute, Chile), and Dr Anthony Addesa (Jupiter Medical Centre, Florida, USA) also participated in this symposium. PMID:27350786

  8. The Risk Assessment Program and the Court of Penal Execution in the Province of Buenos Aires, Argentina.

    PubMed

    Folino, Jorge O; Marengo, Claudio M; Marchiano, Susana E; Ascazibar, Mariel

    2004-02-01

    This study describes the working of a new system that supervises the execution of the punishment phase in the Province of Buenos Aires, Argentina; namely, the Court of Penal Execution, as well as the risk assessment program. It also reports the results of the violent recidivism baseline risk assessment carried out in candidates for conditional release. The cohort studied was recruited during 18 months in the Judicial Department of La Plata, taking into consideration psychopathological and criminological variables. The Psychopathy Checklist-Revised (PCL-R), the Historical, Clinical, and Risk Management--20 (HCR-20), and the Violence Risk Appraisal Guide (VRAG), among others, were used as measures. During the study period, 65 candidates for conditional release were part of the baseline assessment, and they constitute the population of this study. The measures' mean scores were PCL-R, 20.57; HCR-20, 18.58; VRAG, 12.17.

  9. Highlights of the ecancer/SAC First International Prostate Cancer Symposium, 11-12 March 2016, Buenos Aires, Argentina.

    PubMed

    Villalba, Marcelo Blanco; Bramajo, Marina; Bruno, Mario

    2016-01-01

    The ecancer/SAC First International Prostate Cancer Symposium, held in Buenos Aires, included national, regional, and international experts in the field of prostate cancer. More than 200 professionals from a variety of areas (clinical urologists, pathologists, oncologists, biologists, imaging specialists, radiation therapists, and generalist doctors, among others) attended, and they proposed multidisciplinary management of prostate pathology from the start in concordance with the ideas set forth by the organising committee. A radiotherapy workshop was also held during the symposium, in which new techniques and their possible uses were specifically discussed. In addition to the local doctors, Dr Lilian Faroni (COI Group, Rio de Janeiro, Brazil), Dr Leonardo Carmona (Chilean Head and Neck Institute, Chile), and Dr Anthony Addesa (Jupiter Medical Centre, Florida, USA) also participated in this symposium. PMID:27350786

  10. [From educational and health tourism for children to social tourism: vacation camps in the Province of Buenos Aires, Argentina].

    PubMed

    Bruno, Perla

    2015-12-01

    The early twentieth century saw the rise of vacation camps for frail children as educational and health-giving experiences provided by medical and philanthropic organizations. This article analyzes some of these early experiences, seen here as the predecessors of social tourism, in the Province of Buenos Aires. A combination of written sources are examined, mainly institutional reports, periodicals such as the Monitor de la Educación Común - published by the Consejo Nacional de Educación (National Board of Education) - or laws, with photographs and plans for different examples. I argue that these buildings were both physical and cultural "brands" in the places where they were located, and that their architectural structure encapsulated ideas about leisure space and cures in unique natural environments. PMID:26625926

  11. [Sexuality, bodily experiences, and gender: an ethnographic study of persons living with HIV in Greater Metropolitan Buenos Aires, Argentina].

    PubMed

    Grimberg, Mabel

    2009-01-01

    Based on the results of an ethnographic study on daily experience with HIV in Greater Metropolitan Buenos Aires, Argentina, the article discusses behavioral approaches that reduce the sexuality of persons living with HIV to an issue of safety and protection. By articulating a social construction perspective and the notion of hegemony, the author proposes that sexuality can be understood as a process of individual and social construction shaped by power relations and social regulations. The analysis of the experiences of living with HIV in marginalized populations shows how chronic social inequality, violence, discrimination, and stigmatization generate particular characteristics of sexual issues. These social processes become driving forces that shape sexual experience as a field of danger, repression, and restriction rather than pleasure and exploration. Finally, daily confrontation with social metaphors places strain on gender relations, practices, and identities.

  12. Entrainment Heat Flux Computed with Lidar and Wavelet Technique in Buenos Aires During Last Chaitén Volcano Eruption

    NASA Astrophysics Data System (ADS)

    Pawelko, Ezequiel Eduardo; Salvador, Jacobo Omar; Ristori, Pablo Roberto; Pallotta, Juan Vicente; Otero, Lidia Ana; Quel, Eduardo Jaime

    2016-06-01

    At Lidar Division of CEILAP (CITEDEF-CONICET) a multiwavelength Raman-Rayleigh lidar optimized to measure the atmospheric boundary layer is being operated. This instrument is used for monitoring important aerosol intrusion events in Buenos Aires, such as the arrival of volcanic ashes from the Chaitén volcano eruption on May 2008. That was the first monitoring of volcanic ash with lidar in Argentina. In this event several volcanic ash plumes with high aerosol optical thickness were detected in the free atmosphere, affecting the visibility, surface radiation and therefore, the ABL evolution. In this work, the impact of ashes in entrainment flux ratio is studied. This parameter is obtained from the atmospheric boundary layer height and entrainment zone thickness using algorithms based on covariance wavelet transform.

  13. ["I was like a ticking bomb": Experiences of severe maternal morbidity in the Metropolitan Area of Buenos Aires].

    PubMed

    Szulik, Dalia; Szwarc, Lucila

    2015-12-01

    With the objective of recording and analyzing women's experiences with severe maternal morbidity from their perspective, between February and May 2011, 16 semi-structured interviews with women treated in the public hospitals of the Buenos Aires Metropolitan Area who suffered from severe maternal morbidity were carried out. In their testimonies, women report a number of delays in care, such as difficulties in identifying the problem on time, obstacles in accessing health centers and important faults in the management of obstetric emergencies. They describe the event as surprising, distressing and painful, a perception reinforced by the violation of their rights and significant communication problems. These findings are meant as a step towards the holistic and comprehensive study of severe maternal morbidity, as well as to confirm the urgent need for further research from a gender and humans rights perspective. PMID:26676597

  14. [Correlation between results of the residency admission test and of pediatric certification test in Buenos Aires, Argentina].

    PubMed

    Ferreira, Juan Pablo; Hamui, Magali; Paganini, Agustina; Torres, Fernando A; Ossorio, María Fabiana; Eiguchi, Kumiko; Ferrero, Fernando

    2016-01-01

    In the city of Buenos Aires (CABA), pediatric residents enter the residency program after taking a unified admission test. After completion of the program and passing a final test, the Universidad de Buenos Aires (UBA) provides a professional certification. The objective of this study is to determine if the results obtained in the residency admission test (RAT) and those of the professional certification test (PCT) correlated. This is a cross-sectional study, that included all subjects who passed the pediatrics RAT in CABA in 2004-2009, and that attended the pediatric PCT of the UBA. The score for each subject in both tests was obtained and the corresponding correlation was calculated. Results were divided in quintiles, and the proportion of subjects who improved their position in the PCT with respect to the RAT was calculated. Data from 303 subjects was obtained. The RAT showed a median of 45.0 (over 60 maximum) (IC-range: 43.0-48.7), and the PCT showed a median of 6 points (over 10 max.)(IC-range: 6-8). A significative correlation between results in RAT and PCT was observed (r = 0.37, p < 0.001). Based on their position in the RAT, 43.8% of subjects improved their position in the PCT, without differences between residents attending pediatric and general hospitals (45.6 vs. 31.5%; p = 0.1). In the case of pediatric residents, results of the residency admission test correlate with those obtained in the professional certification test. PMID:27295703

  15. Assessment of the possible drought impact on farm production in the SE of the province of Buenos Aires, Argentina

    NASA Astrophysics Data System (ADS)

    Hartmann, Tomas; Di Bella, Carlos; Oricchio, Patricio

    The extended drought situation in the southeast of Buenos Aires during the second half of year 2000 caused the government to invoke emergency laws. This action allowed farmers in the area to receive waivers for taxes and loans. The emergency laws remained in force during 2001, without further verification of environmental conditions for agriculture. Developing an assessment of the actual drought situation was relevant for taxing and national credit institutions. An assessment was performed of the actual drought situation of farms during the spring of 2001 in seven counties in Buenos Aires Province area. The assessment was done by comparing vegetation index values (NDVI)—as measured from NOAA-AVHRR satellite data—of September 2001 against NDVI time series values from previous years. Five categories were established to describe the relationship between the present index and the average of the time series. Farms within the area covered by the study were assigned to the appropriate category using GIS tools. It was confirmed that most of the area had NDVI values that were similar to the average values, or even higher. It was found that there were subareas where the vegetation index had decreased. For those cases, LANDSAT TM images of the area of September and October of 2001 were used for a detailed inspection. The study included rainfall data as well, confirming a normal regional situation. Both low and high-resolution satellite images were found to be useful tools for obtaining fast, economic, objective and conclusive results about the production capability of individual farms as well as the region as a whole.

  16. Distribution study of Chlamydia trachomatis genotypes in symptomatic patients in Buenos Aires, Argentina: association between genotype E and neonatal conjunctivitis

    PubMed Central

    2010-01-01

    Background Chlamydia trachomatis infections are the most prevalent sexually transmitted bacterial infections in the world. There is scarce data available referring to the distribution of C. trachomatis genotypes in Argentina. The aim of this study was to identify the genotypes of C. trachomatis circulating in the metropolitan area of Buenos Aires (Argentina) associated with ophthalmia neonatorum and genital infections. Findings From 2001 to 2006, 199 positive samples for C. trachomatis infection from symptomatic adult patients and neonates with ophthalmia neonatorum from two public hospitals were studied. C. trachomatis genotypes were determined by PCR-RFLP of an ompA fragment. Genotype E was the most prevalent regardless of the sample origin (46.3% 57/123 in adults and 72.4% 55/76 in neonates), followed by genotype D (19.5% 24/123) and F (14.6% 18/123) in adults, and G (9.2% 7/76) and D (7.9% 6/76) in neonates. We detected a significantly higher frequency of genotype E (p < 0.001, OR = 3.03 (1.57Buenos Aires, Argentina, in which genotypes E, D and F were the predominant ones. PMID:20181127

  17. Comments on Environmental and Sanitary Aspects of the Scorpionism by Tityus trivittatus in Buenos Aires City, Argentina.

    PubMed

    de Roodt, Adolfo Rafael

    2014-04-01

    Deaths by venomous animals are medical emergencies that can lead to death and thus constitute sanitary problems in some regions of the world. In the South of America, the accidents by these animals are a common sanitary problem especially in warm, tropical or subtropical regions, related with rural work in several countries. Argentina is located in the extreme South of South America and a minor part of the continental surface is in tropical or subtropical regions, where most of the accidents by venomous animals happen. However, in the big cities in the center and South of the country, with no relation to rural work, scorpionism, mostly due to the synanthropic and facultative parthenogenetic scorpion Tityus trivittatus, has become a sanitary problem in the last few decades. This scorpion is present in the biggest cities of Argentina and in the last decades has killed over 20 children in provinces of the center and north of the country, mostly in big cities. In addition, it seems that this species is growing and spreading in new regions of the cities. In this revision, some characteristics of this scorpion regarding its habitat, spreading in Buenos Aires city, combat measures and available treatments are discussed. PMID:24759176

  18. Embryonic and Morphological Development of Larvae and Juvenile of the Buenos Aires Tetra, Hyphessobrycon anisitsi (Pisces Characidae) Characidae Fishes.

    PubMed

    Park, Jae-Min; Han, Kyeong-Ho; Han, Ran

    2015-03-01

    We have launched an investigation for Embryonic Development, Larvae and Juvenile Morphology, of Buenos aires tetra in order to build basic data of Characidae and fish seeding production. We brought 50 couples of Characidae from Bizidduck aquarium in Yeosu-si, Jeollanamdo, from Korea on March of 2015. We put them in the tetragonal glass aquarium (50×50×30 cm). Breeding water temperature was 27.5~28.5°C (mean 28.0±0.05°C) and being maintained. The shape of fertilized egg was round shape, and it was adhesive demersal egg. The egg size was 0.63~0.91 mm (mean 0.74±0.07 mm, n=20). After getting fertilized egg, the developmental stage was gastrula stage, and embryo covered almost two-thirds of Yolk. Incubation was happened after 16 hours 13 minutes from gastrula stage, and the tail of juvenile came out first with tearing egg capsule. Immediately after the incubation, prelarvae had 3.78~3.88 mm length (mean 3.84±0.04 mm, n=5), and it had no mouth and anus yet. 34 days after hatching from the incubation, juvenile had 8.63~13.1 mm (mean 10.9±1.66 mm), and it had similar silver-colored body shape with its mother.

  19. New toxics, new poverty: a social understanding of the freebase cocaine/Paco in Buenos Aires, Argentina.

    PubMed

    Epele, María E

    2011-01-01

    Included within the field of research on changes in drug use patterns and vulnerability are conditions of emergency related to economic crisis, wars, and political conflict. This study addresses the complex connections between the rapid propagation of freebase cocaine (FBC)-locally known as "pasta base" or "Paco" in Argentina and the normalization of the consequences of Argentina's 2001-2002 political-economic crisis. On the basis of the results of an ethnographic study carried out in three neighborhoods of the Greater Buenos Aires area between 2001 and 2005, this article aims to analyze how changes in the material and social living conditions are interrelated with the high toxicity of FBC/Paco and engender the emerging compulsion of its consumption and deterioration to the bodies, subjectivities, and social activities of active drug users from these shantytowns. By analyzing the changes in transactions directly or indirectly involving drugs-specifically those ranging from cocaine to FBC/Paco-we can argue how structural poverty, "new poverty," is not only associated with the expansion of FBC/Paco but is also shaped by its use, modes of consumption, associated health problems, and sufferings.

  20. The glacial geomorphology of the Lago Buenos Aires and Lago Puerreydón ice lobes, Central Patagonia

    NASA Astrophysics Data System (ADS)

    Bendle, Jacob; Thorndycraft, Varyl; Palmer, Adrian

    2016-04-01

    Patagonia is ideally located for reconstructions of late Quaternary ice-climate interaction(s) in the Southern Hemisphere mid-latitudes, yet many questions remain concerning post-LGM ice sheet retreat dynamics across the region. While modern-day glaciation is restricted to three small icefields (the North and South Patagonian and Cordillera Darwin icefields), during the Quaternary, and at the LGM, episodes of significant ice advance culminated in an expansive Patagonian ice sheet (PIS) centered over the southern Andes, for which a long and well-preserved landform record exists. Previous mapping in the region has either aimed to achieve regional coverage, necessarily omitting more subtle/complex features suggestive of certain ice-marginal processes, or has focused on the identification of palaeo-ice limits (e.g. moraine ridges) for geochronological applications, with little attention given to other (e.g. glaciofluvial, glaciolacustrine) features that are significant for understanding post-LGM ice sheet retreat dynamics. This poster presents a comprehensive and highly detailed (<30m spatial resolution) map of the glacial geomorphology of the Lago Buenos Aires (46.4°S) and Lago Puerreydón (47.2°S) ice lobes, major outlet glaciers of the central sector of the former PIS. The map allows refined reconstructions of glacial and, in particular, deglacial ice-marginal processes, and will underpin further analysis on the retreat history of the palaeo-ice lobes using high-resolution lithostratigraphic (varve) analyses.

  1. Implementation in Buenos Aires City of a program to prevent neurological damage caused by hypoxic-ischemic encephalopathy: Therapeutic hypothermia.

    PubMed

    Valera, Mariana; Berazategui, Juan Pablo; Saa, Gladys; Olmo Herrera, Carolina; Sepúlveda, Teresa; Buraschi, María Fernanda; Gacio, Sebastián; Villalba, Cristina; Beloso, Inés; Basso, Graciela; Carlo, Waldemar; Tavosnanska, Jorge

    2015-10-01

    Therapeutic hypothermia is the standard of care for hypoxic-ischemic encephalopathy (HIE). This treatment was implemented at a regional level by the perinatal network of the City of Buenos Aires. The following are the objectives of this article: 1. To describe the implementation of the network's hypothermia treatment program; 2. To report treatment-associated complications, adverse events and mortality. The program was implemented in stages: 1) 2009-2010. Training and instruction on how to use the equipment. 2) 20102014. Treatment and follow-up of patients with moderate or severe HIE. Up to October 2014, 27 newborn infants received hypothermia treatment with moderate (n= 15) and severe (n= 12) HIE. None of the patients died during treatment. Three newborn infants were lost to follow-up. Among the 16 survivors older than one year old, three have severe neurological disability. Program implementation was plausible. It is imperative to train health care providers on how to identify patients with HIE. PMID:26294149

  2. [Social inequalities in cervical cancer mortality in the Autonomous City of Buenos Aires, 1999-2003 and 2004-2006].

    PubMed

    Martínez, María Laura; Guevel, Carlos Gust

    2013-08-01

    The aim of this study was to describe the spatial distribution of cervical cancer mortality in the Autonomous City of Buenos Aires during the period 1999-2003 and its relationship to the socioeconomic conditions of the population, as well as to compare the distribution during this period with that of the triennium 2004-2006. This ecological study used electoral districts as the unit of analysis. The selected socioeconomic indicators were educational deficit, lack of health insurance and the Material Deprivation of Households Index (Índice de Privación Material de Hogares), taken from the National Population and Housing Census (Censo Nacional de Población, Hogares y Viviendas) of 2001. The stratification of the city into areas according to these conditions and the analysis of standardized mortality ratios showed an increased risk of dying from cervical cancer associated with worse socioeconomic conditions. The stratification and death risks demonstrated a clear spatial pattern, with the south of the city presenting the highest death risks, and the northern and central areas presenting the lowest risks. PMID:23989624

  3. Embryonic and Morphological Development of Larvae and Juvenile of the Buenos Aires Tetra, Hyphessobrycon anisitsi (Pisces Characidae) Characidae Fishes

    PubMed Central

    Park, Jae-Min; Han, Kyeong-Ho; Han, Ran

    2015-01-01

    We have launched an investigation for Embryonic Development, Larvae and Juvenile Morphology, of Buenos aires tetra in order to build basic data of Characidae and fish seeding production. We brought 50 couples of Characidae from Bizidduck aquarium in Yeosu-si, Jeollanamdo, from Korea on March of 2015. We put them in the tetragonal glass aquarium (50×50×30 cm). Breeding water temperature was 27.5~28.5°C (mean 28.0±0.05°C) and being maintained. The shape of fertilized egg was round shape, and it was adhesive demersal egg. The egg size was 0.63~0.91 mm (mean 0.74±0.07 mm, n=20). After getting fertilized egg, the developmental stage was gastrula stage, and embryo covered almost two-thirds of Yolk. Incubation was happened after 16 hours 13 minutes from gastrula stage, and the tail of juvenile came out first with tearing egg capsule. Immediately after the incubation, prelarvae had 3.78~3.88 mm length (mean 3.84±0.04 mm, n=5), and it had no mouth and anus yet. 34 days after hatching from the incubation, juvenile had 8.63~13.1 mm (mean 10.9±1.66 mm), and it had similar silver-colored body shape with its mother. PMID:25949207

  4. Ecotoxicological studies of environmental samples from Buenos Aires area using a standardized amphibian embryo toxicity test (AMPHITOX).

    PubMed

    Herkovits, Jorge; Perez-Coll, Cristina; Herkovits, Francisco D

    2002-01-01

    The toxicity of 34 environmental samples from potentially polluted and reference stations were evaluated by means of the AMPHITOX test from acute to chronic exposure according to the toxicity found in each sample. The samples were obtained from surface and ground water, leaches, industrial effluents and soils. The data, expressed in acute, short-term chronic and chronic Toxicity Units (TUa, TUstc and TUc) resulted in a maximal value of 1000 TUc, found in a leach, while the lower toxicity value was 1.4 TUa corresponding to two surface water samples. In five samples (four providing from reference places) no toxicity was detected. The results point out the possibility of evaluating the toxicity of a wide diversity of samples by means of AMPHITOX as a customized toxicity test. The fact that almost all samples with suspected toxicity in rivers and streams from the Metropolitan area of Buenos Aires city resulted toxic, indicates the need of enhanced stewardship of chemical substances for environmental and human health protection purposes. PMID:11808551

  5. An Assessment of Spontaneous Vegetation Recovery in Aggregate Quarries in Coastal Sand Dunes in Buenos Aires Province, Argentina

    NASA Astrophysics Data System (ADS)

    Fernández Montoni, María Victoria; Fernández Honaine, Mariana; del Río, Julio Luis

    2014-08-01

    Sand dune quarries are a location of common aggregate mining activity developed in coastal areas, especially in the southeast Buenos Aires province, Argentina. In this article, spontaneous plant development after extraction activity ceased was evaluated. Five areas (three quarried and two natural/conservation areas) were sampled for plant cover and composition as well as sediment characterization. Different indexes, principal component analysis, and cluster analyses were applied to compare the areas. The dominant families observed in four of the five areas were Asteraceae, Poaceae, and Cyperaceae, and most of the species are commonly found in sandy and humid soils and/or modified/anthropized ones. Percentages of plant cover increased with time because of the cessation of active aggregate extraction. Indexes and multivariate analyses showed that it was possible to distinguish quarried and natural areas based on composition and vegetation cover. The distribution of plant species among the four areas responded to the presence of mining activity, but it also responded to the topographical position and consequently the depth of the groundwater level. Besides these differences, the four areas shared many native species. The results might indicate that once the activity has ceased, quarried areas may spontaneously and quickly develop a plant community with some similarities to those present in the nonquarried areas. However, given that the extracting activity involves the removal of the soil, revegetation of this type of environment depends on the presence of natural areas in the surroundings, which can serve as a source of seeds and propagules for plant regeneration.

  6. Antiquity of the Rı´o de la Plata craton in Tandilia, southern Buenos Aires province, Argentina

    NASA Astrophysics Data System (ADS)

    Pankhurst, R. J.; Ramos, A.; Linares, E.

    2003-05-01

    Rb-Sr and Sm-Nd whole-rock data for granitoids and orthogneisses from the western part of the Sierras Septentrionales of the southern Buenos Aires province yield an errorchron of 2009±71 Ma (initial 87Sr/ 86Sr=0.7041, MSWD=69) and an isochron of 2140±88 Ma (initial 143Nd/ 144Nd=0.50977), respectively. As in previous investigations, the Rb-Sr data are clearly disturbed, but the Sm-Nd isochron may record the age of emplacement of igneous precursors. These results reaffirm that this region is the southern extension of the crystalline basement of the Rı´o de la Plata craton. The Sm-Nd age, though not very precise, is slightly older than previously demonstrated but consistent with most recent U-Pb studies of the craton exposed in Uruguay and Brazil. Crust-derived Sm-Nd model ages averaging 2620±80 Ma indicate that, though the principal rock-forming events were Paleoproterozoic, a Late Archaean prehistory is possible. However, the data place strict constraints on the nature and intensity of post-2000 Ma activity in this area, which seems to be confined to tholeiitic dyke emplacement and hydrothermal reactivation.

  7. Biostratigraphy and biochronology of the Monte Hermoso Formation (early Pliocene) at its type locality, Buenos Aires Province, Argentina

    NASA Astrophysics Data System (ADS)

    Tomassini, Rodrigo L.; Montalvo, Claudia I.; Deschamps, Cecilia M.; Manera, Teresa

    2013-12-01

    The Monte Hermoso Formation, cropping out at its type locality of Farola Monte Hermoso (Buenos Aires Province), is a classical fossiliferous unit of the South American Neogene, highlighted by the abundance and diversity of its vertebrate remains. However, its biostratigraphy and age have been largely debated, and numerous discrepancies and controversies have been stated. In this regard, the result of the analysis of new materials recovered from the different levels of this formation, following a strict control of stratigraphic provenance, is here reported. As well, the provenance of specimens of previous collections has been evaluated. The studied assemblage consists of Osteichthyes, Amphibia, Reptilia, Aves and Mammalia. These latter are the most numerous and belong to the Didelphimorphia, Polydolopimorphia, Rodentia, Notoungulata, Litopterna and Xenarthra. The recorded taxa suggest no important faunistic variations among the different levels of the Monte Hermoso Formation that would imply significant chronological differences, and hence, justify the recognition of two biostratigraphic units. The analysis of the first and last records as well as the taxa considered as exclusive, does not support the validity of the biozones of Trigodon gaudryi and Neocavia depressidens previously proposed. On this basis, a new scheme for the Monte Hermoso Formation at its type locality is proposed, including a new single biostratigraphic unit. This unit is the Eumysops laeviplicatus Range Zone, which represents the biostratigraphic base for the Montehermosan Stage/Age of the early Pliocene.

  8. Winter survival of immature instars of Mansonia indubitans Dyar & Shannon and Mansonia titillans Walker (Diptera: Culicidae), in Buenos Aires, Argentina.

    PubMed

    Torretta, Juan P; Mulieri, Pablo R; Patitucci, Luciano D; Sander, Valeria A; Rodríguez, Patricia L; Schweigmann, Nicolás

    2006-09-01

    We conducted a whole year research on the ecology of Mansonia indubitans and Ma. titillans in Macáes Pond, Costanera Sur Reserve, Buenos Aires, Argentina. The usage of different floating plants by immature instars and their overwintering was analyzed. The percentage of usage of the available floating macrophytes (Pistia, Limnobium, and Salvinia) by the larvae and pupae was studied. Also, we defined positivity (P+) as the percentage of plants with immature instars for each plant genus on a monthly basis. Ma. immature instars were captured throughout the year and Pistia was the resource most commonly exploited by the mosquitoes. The percentage of fourth-instar larvae and pupae on Pistia roots with respect to total immature instars captured was assessed on a monthly and seasonal basis. The proportion of fourth-instar larvae and pupae from both species of Mansonia on water lettuce roots, showed significant differences between months and seasons. Our results suggest that the populations of Ma. indubitans and Ma. titillans in Macáes Pond, survive during winter mainly as fourth-instar larvae. PMID:17072469

  9. Characterization and quantitation of parasite species in the effluents of the Berisso main sewage channel, Buenos Aires, Argentina.

    PubMed

    De Luca, M M; Pezzani, B C; Córdoba, M A; Basualdo, J A

    1997-10-01

    Outbreaks of disease can be caused by pathogenic intestinal parasites in drinking water. La Plata, the capital of the province of Buenos Aires, Argentina, is located on the La Plata River; a principal tributary receiving the raw sewage from the city's 500,000 inhabitants via the Berisso main septic channel at an estimated volume of 108,390 m3/day (4516.3m3/h). To determine the parasite-contamination level of the La Plata River, we obtained 18 sewage effluents from the Berisso main septic channel at its point of discharge into the river and concentrated these samples by filtration. This approach detected the following parasites at the indicated average levels (cysts/m3): members of the flagellate genus Giardia, more than 9000; representatives of the amoebic species Entamoeba histolytica, slightly more than 800 and Entamoeba coli, 5000; other amoebic forms, up to 23,000. These data clearly illustrate the degree of contamination within the La Plata River caused by the Berisso septic channel. Pollution of the La Plata River to this extent thus presents a high health risk for people who drink the water from this river even after conventional processing as well as for those who use the water from this portion of the river for recreation.

  10. Comments on Environmental and Sanitary Aspects of the Scorpionism by Tityus trivittatus in Buenos Aires City, Argentina

    PubMed Central

    de Roodt, Adolfo Rafael

    2014-01-01

    Deaths by venomous animals are medical emergencies that can lead to death and thus constitute sanitary problems in some regions of the world. In the South of America, the accidents by these animals are a common sanitary problem especially in warm, tropical or subtropical regions, related with rural work in several countries. Argentina is located in the extreme South of South America and a minor part of the continental surface is in tropical or subtropical regions, where most of the accidents by venomous animals happen. However, in the big cities in the center and South of the country, with no relation to rural work, scorpionism, mostly due to the synanthropic and facultative parthenogenetic scorpion Tityus trivittatus, has become a sanitary problem in the last few decades. This scorpion is present in the biggest cities of Argentina and in the last decades has killed over 20 children in provinces of the center and north of the country, mostly in big cities. In addition, it seems that this species is growing and spreading in new regions of the cities. In this revision, some characteristics of this scorpion regarding its habitat, spreading in Buenos Aires city, combat measures and available treatments are discussed. PMID:24759176

  11. Factors associated with infection by Campylobacter fetus in beef herds in the Province of Buenos Aires, Argentina.

    PubMed

    Jimenez, D F; Perez, A M; Carpenter, T E; Martinez, A

    2011-09-01

    Campylobacter fetus is a major venereal pathogen of cattle that is considered to be widespread among the livestock population of Argentina. The disease accounts for a 10% reduction in the weaning rate of Argentine infected herds and annual losses of $165 million. A case-control, questionnaire-based study was developed with the objective of quantifying the association between C. fetus infection and demographic, husbandry, and sanitary factors in 196 herds located in the province of Buenos Aires, Argentina. Abortions observed in the herd (OR=3.08, 95% CI=1.52, 6.23), and trespassing of bulls from neighboring herds (OR=2.03, 95% CI=0.98, 4.20), were positively associated with the risk of finding C. fetus-infected bulls, whereas buying bulls was a protective factor for the disease (OR=0.53, 95% CI=0.26, 1.08). Results presented here will help to develop and implement actions aimed at preventing the spread and reducing the incidence of C. fetus infection in the beef cattle population of Argentina. PMID:21737166

  12. U-Th and 10Be constraints on sediment recycling in proglacial settings, Lago Buenos Aires, Patagonia

    NASA Astrophysics Data System (ADS)

    Cogez, Antoine; Herman, Frédéric; Pelt, Eric; Norton, Kevin; Darvill, Christopher; Christl, Marcus; Morvan, Gilles; Reuschlé, Thierry; Chabaux, François

    2016-04-01

    The sedimentary cycle includes the formation by erosion of rocks, transport and deposition. While erosion and deposition can be documented, the history of sediments between the time it is extracted from the rocks and ultimately deposited into basins remains a major challenge. However, the mechanism of transfer and alteration of the sediments during transport plays a key role in the evolution of basins, feedbacks between erosion and climate, and glacial-interglacial variability of sediment transport and weathering. This is particularly true in proglacial settings because large overdeepenings, in particular, are potential sediment traps for which the efficiency at evacuating those sediments is largely unknown. The Lago Buenos Aires moraines in Patagonia are particularly interesting because they are imbricated from the older in the outer part to the younger in the inner part of the system. We sampled fine grained sediments from these moraines and measured U-Th isotopes in the 4-50 μm silicate fraction. Deposition ages were refined using 10Be exposure ages. We show first that the comminution ages model can be improved by measuring also Th isotopes, from which weathering rates can be deduced. Moreover we show from our data that there is a time lag of 300 kyr on average between erosion and deposition in the moraine. This could be attributed to the long residence time of sediments in the lake overdeepening. This conclusion raises perspectives about the transport times and dynamic of the sediments during a whole sedimentary cycle, and the subsequent effect on weathering. This conclusion could also contradict some assumptions commonly made for our erosion rates/sediment fluxes reconstructions based on river sediments analysis, in recently deglaciated catchments.

  13. [Genomic heterogeneity of hepatitis B virus, genotype A circulating in the metropolitan area of Buenos Aires, Argentina].

    PubMed

    Fernandez, C; Mariana; de Torres, R A

    1996-01-01

    HVB DNA was extracted from highly purified Dane particles, from sera of HBV viremic patients, collected in the metropolitan area of Buenos Aires (Argentina). HBV DNA was cloned in pUC18 vector, amplified in Escherichia coli DH5 F'. Plasmids were recovered and analyzed for HBV DNA inserts. Three recombinant plasmids, pHB4, pHB7 and pHB20 were selected, and HBVDNA inserts sequenced. The resulted sequences were incorporated at the GenBank, with the following accession numbers: PHB4P3=U33188; PHB4P5=U33189; PHB7P3=U33190; PHB7P5=U33191 and PHB20=U33190; PHB7P5=U33191 and PHB20=U33187. All belongs to the genotype A, pHB4 and pHB20 have a very close relation in between each other and with L13994 sequence, from North America origin. pHB7 have a significant distance from pHB4 and pHB20 and have a discrete homology with m57633 detected in Philippines. pHB4 shows a mutation at the T 3182-Leu in the preS1 region that change Pro for Leu, this mutation is absent in 125 sequences selected (having a 65% or more of homology) from NCBI by Blast algortm. The sequence of the pre C regions of all three inserts do not show any evidence to belong to the e-or scape mutants. Type A genotypes shows to be common in the area, but a hight degree of divergence have been demonstrate between two circulating strains. PMID:9335928

  14. Increasing incidence of multiple sclerosis among women in Buenos Aires: a 22 year health maintenance organization based study.

    PubMed

    Cristiano, E; Patrucco, L; Miguez, J; Giunta, D; Peroni, J; Rojas, J I

    2016-10-01

    Studies in multiple sclerosis (MS) suggest a trend of increasing disease prevalence and incidence, and especially, a disproportional increase in the incidence of multiple sclerosis in women. The objective of this study was to evaluate the incidence of MS over 22 years and to determine the ratio in incidence of men to women in a health maintenance organization from Buenos Aires, Argentina. The population was made up of all members of a hospital-based HMO affiliated between January 1992 and December 2013. Each person was followed contributing time at risk. Cases with definite diagnosis of MS were included. Incidence density was calculated with 95 % confidence intervals and compared between women and men. 165,456 subjects were followed for a total of 1,488,575 person-years, of whom 42 developed MS. Incidence density was 3/100,000 person-years (95 % CI 2.1-3.5/100,000 person-years). During this period (1992-2013), the incidence rate in women increased from 1/100,000 (95 % CI 0.8-1.6) to 4.9/100,000 (95 % CI 4.1-5.4) (p < 0.001), while in men the incidence ranged from 1.4/100,000 (95 % CI 1-1.7) to 1.8 (1.3-2.1) (p = 0.16). Incidence density during the study period increased significantly in women but not in men. This is the first report of this phenomenon in Latin America region.

  15. Phytoplankton chlorophyte structure as related to ENSO events in a saline lowland river (Salado River, Buenos Aires, Argentina).

    PubMed

    Solari, Lía C; Gabellone, Néstor A; Claps, María C; Casco, María A; Quaíni, Karina P; Neschuk, Nancy C

    2014-04-01

    We analyzed the phytoplankton present in the lower sector of the Salado River (Buenos Aires, Argentina) for 10 years (1995-2005) and detected significant changes occurring in chlorophyte abundance and species richness during La Niña event (1998-1999), which period was analyzed throughout the entire basin (main stream and tributaries). We compared the physicochemical and biologic variables between two El Niño-La Niña-Southern Oscillation (ENSO) periods - El Niño (March 1997-January 1998) and La Niña (May 1998-May 1999) - to identify possible indicators of a relationship between climatic anomalies and chlorophyte performance. Chlorophyte density increased during the La Niña. Under normal or extreme hydrologic conditions, mobile (Chlamydomonas spp.) and nonmobile (Monoraphidium spp.) chlorophytes codominated. These species belonged to Reynolds's functional groups X1 and X2, those typical of nutrient-enriched environments. Comparative analyses between El Niño and La Niña periods indicated significant differences in physicochemical (K(+), dissolved polyphenols, particulate reactive phosphorus, alkalinity, pH) and biologic (species diversity and richness, phytoplankton and chlorophyte total densities) variables between the two periods at all basin sites. During the La Niña condition, species richness was greater owing to interconnected shallow lakes and drainage-channel inputs, while the Shannon diversity index was lower because of the high abundance values of Monoraphidium minutum. A detailed analysis of the chlorophytes in the entire basin, indicated that changes in density and species dominance occurred on a regional scale although diverse chlorophyte assemblages were identified in the different sectors of the Salado River basin. After La Niña event, the entire basin had the potential to revert to the previous density values, showing the resilience to global environmental changes and the ability to reestablish the general conditions of stability. PMID

  16. Childhood Sexual Experiences with an Older Partner among Men Who Have Sex with Men in Buenos Aires, Argentina

    PubMed Central

    Dolezal, Curtis; Carballo-Diéguez, Alex; Balán, Iván C.; Pando, María A.; Mabragaña, Marina; Marone, Rubén; Barreda, Victoria; Avila, María M.

    2013-01-01

    This study sought to describe childhood sexual experiences with older partners (CSEOP) among men who have sex with men (MSM) in Buenos Aires, Argentina. MSM were recruited through Respondent Driven Sampling. They responded to a computer administered self-interview with questions on CSEOP, operationalized as manual, oral, genital, or anal contact prior to age 13 with a partner at least 4 years older. Of the 500 respondents, only 25% identified as gay. Eighteen percent of the respondents reported CSEOP, the majority of whom did not feel they were hurt by the experience and did not consider it to be childhood sexual abuse (CSA). Over two-thirds of MSM who reported CSEOP said that their older partner was a female. Only 4% of those with a female partner felt their experience was CSA compared to 44% of those who had a male partner. Among all men reporting CSEOP, those who felt sexually abused were more likely to have been physically forced or threatened, physically hurt, and emotionally hurt than those who did not feel sexually abused. Having CSEOP, being hurt by the experiences, and perceiving the experiences as sexual abuse were not associated with current HIV sexual risk or substance use behavior. In this sample of MSM in Argentina, a substantial minority reported CSEOP. Those who felt they had been sexually abused were much more likely to have had an older male partner than an older female partner, and were more likely to report having been physically forced and threatened by their older partner. PMID:24210270

  17. Childhood sexual experiences with an older partner among men who have sex with men in Buenos Aires, Argentina.

    PubMed

    Dolezal, Curtis; Carballo-Diéguez, Alex; Balán, Iván C; Pando, María A; Mabragaña, Marina; Marone, Rubén; Barreda, Victoria; Avila, María M

    2014-02-01

    This study sought to describe childhood sexual experiences with older partners (CSEOP) among men who have sex with men (MSM) in Buenos Aires, Argentina. MSM were recruited through respondent driven sampling. They responded to a computer administered self-interview with questions on CSEOP, operationalized as manual, oral, genital, or anal contact prior to age 13 with a partner at least 4 years older. Of the 500 respondents, only 25% identified as gay. Eighteen percent of the respondents reported CSEOP, the majority of whom did not feel they were hurt by the experience and did not consider it to be childhood sexual abuse (CSA). Over two-thirds of MSM who reported CSEOP said that their older partner was a female. Only 4% of those with a female partner felt their experience was CSA compared to 44% of those who had a male partner. Among all men reporting CSEOP, those who felt sexually abused were more likely to have been physically forced or threatened, physically hurt, and emotionally hurt than those who did not feel sexually abused. Having CSEOP, being hurt by the experiences, and perceiving the experiences as sexual abuse were not associated with current HIV sexual risk or substance use behavior. In this sample of MSM in Argentina, a substantial minority reported CSEOP. Those who felt they had been sexually abused were much more likely to have had an older male partner than an older female partner, and were more likely to report having been physically forced and threatened by their older partner. PMID:24210270

  18. [Genomic heterogeneity of hepatitis B virus, genotype A circulating in the metropolitan area of Buenos Aires, Argentina].

    PubMed

    Fernandez, C; Mariana; de Torres, R A

    1996-01-01

    HVB DNA was extracted from highly purified Dane particles, from sera of HBV viremic patients, collected in the metropolitan area of Buenos Aires (Argentina). HBV DNA was cloned in pUC18 vector, amplified in Escherichia coli DH5 F'. Plasmids were recovered and analyzed for HBV DNA inserts. Three recombinant plasmids, pHB4, pHB7 and pHB20 were selected, and HBVDNA inserts sequenced. The resulted sequences were incorporated at the GenBank, with the following accession numbers: PHB4P3=U33188; PHB4P5=U33189; PHB7P3=U33190; PHB7P5=U33191 and PHB20=U33190; PHB7P5=U33191 and PHB20=U33187. All belongs to the genotype A, pHB4 and pHB20 have a very close relation in between each other and with L13994 sequence, from North America origin. pHB7 have a significant distance from pHB4 and pHB20 and have a discrete homology with m57633 detected in Philippines. pHB4 shows a mutation at the T 3182-Leu in the preS1 region that change Pro for Leu, this mutation is absent in 125 sequences selected (having a 65% or more of homology) from NCBI by Blast algortm. The sequence of the pre C regions of all three inserts do not show any evidence to belong to the e-or scape mutants. Type A genotypes shows to be common in the area, but a hight degree of divergence have been demonstrate between two circulating strains.

  19. Phytoplankton chlorophyte structure as related to ENSO events in a saline lowland river (Salado River, Buenos Aires, Argentina)

    PubMed Central

    Solari, Lía C; Gabellone, Néstor A; Claps, María C; Casco, María A; Quaíni, Karina P; Neschuk, Nancy C

    2014-01-01

    We analyzed the phytoplankton present in the lower sector of the Salado River (Buenos Aires, Argentina) for 10 years (1995–2005) and detected significant changes occurring in chlorophyte abundance and species richness during La Niña event (1998–1999), which period was analyzed throughout the entire basin (main stream and tributaries). We compared the physicochemical and biologic variables between two El Niño–La Niña–Southern Oscillation (ENSO) periods – El Niño (March 1997–January 1998) and La Niña (May 1998–May 1999) – to identify possible indicators of a relationship between climatic anomalies and chlorophyte performance. Chlorophyte density increased during the La Niña. Under normal or extreme hydrologic conditions, mobile (Chlamydomonas spp.) and nonmobile (Monoraphidium spp.) chlorophytes codominated. These species belonged to Reynolds's functional groups X1 and X2, those typical of nutrient-enriched environments. Comparative analyses between El Niño and La Niña periods indicated significant differences in physicochemical (K+, dissolved polyphenols, particulate reactive phosphorus, alkalinity, pH) and biologic (species diversity and richness, phytoplankton and chlorophyte total densities) variables between the two periods at all basin sites. During the La Niña condition, species richness was greater owing to interconnected shallow lakes and drainage-channel inputs, while the Shannon diversity index was lower because of the high abundance values of Monoraphidium minutum. A detailed analysis of the chlorophytes in the entire basin, indicated that changes in density and species dominance occurred on a regional scale although diverse chlorophyte assemblages were identified in the different sectors of the Salado River basin. After La Niña event, the entire basin had the potential to revert to the previous density values, showing the resilience to global environmental changes and the ability to reestablish the general conditions of

  20. Phytoplankton chlorophyte structure as related to ENSO events in a saline lowland river (Salado River, Buenos Aires, Argentina).

    PubMed

    Solari, Lía C; Gabellone, Néstor A; Claps, María C; Casco, María A; Quaíni, Karina P; Neschuk, Nancy C

    2014-04-01

    We analyzed the phytoplankton present in the lower sector of the Salado River (Buenos Aires, Argentina) for 10 years (1995-2005) and detected significant changes occurring in chlorophyte abundance and species richness during La Niña event (1998-1999), which period was analyzed throughout the entire basin (main stream and tributaries). We compared the physicochemical and biologic variables between two El Niño-La Niña-Southern Oscillation (ENSO) periods - El Niño (March 1997-January 1998) and La Niña (May 1998-May 1999) - to identify possible indicators of a relationship between climatic anomalies and chlorophyte performance. Chlorophyte density increased during the La Niña. Under normal or extreme hydrologic conditions, mobile (Chlamydomonas spp.) and nonmobile (Monoraphidium spp.) chlorophytes codominated. These species belonged to Reynolds's functional groups X1 and X2, those typical of nutrient-enriched environments. Comparative analyses between El Niño and La Niña periods indicated significant differences in physicochemical (K(+), dissolved polyphenols, particulate reactive phosphorus, alkalinity, pH) and biologic (species diversity and richness, phytoplankton and chlorophyte total densities) variables between the two periods at all basin sites. During the La Niña condition, species richness was greater owing to interconnected shallow lakes and drainage-channel inputs, while the Shannon diversity index was lower because of the high abundance values of Monoraphidium minutum. A detailed analysis of the chlorophytes in the entire basin, indicated that changes in density and species dominance occurred on a regional scale although diverse chlorophyte assemblages were identified in the different sectors of the Salado River basin. After La Niña event, the entire basin had the potential to revert to the previous density values, showing the resilience to global environmental changes and the ability to reestablish the general conditions of stability.

  1. Aerosol properties and meteorological conditions in the city of Buenos Aires, Argentina, during the resuspension of volcanic ash from the Puyehue-Cordón Caulle eruption

    NASA Astrophysics Data System (ADS)

    Graciela Ulke, Ana; Torres Brizuela, Marcela M.; Raga, Graciela B.; Baumgardner, Darrel

    2016-09-01

    The eruption in June 2011 of the Puyehue-Cordón Caulle Volcanic Complex in Chile impacted air traffic around the Southern Hemisphere for several months after the initial ash emissions. The ash deposited in vast areas of the Patagonian Steppe was subjected to the strong wind conditions prevalent during the austral winter and spring experiencing resuspension over various regions of Argentina. In this study we analyze the meteorological conditions that led to the episode of volcanic ash resuspension which impacted the city of Buenos Aires and resulted in the closure of the two main airports in Buenos Aires area (Ezeiza and Aeroparque) on 16 October 2011. A relevant result is that resuspended material (volcanic ash plus dust) imprints a distinguishable feature within the atmospheric thermodynamic vertical profiles. The thermodynamic soundings show the signature of "pulses of drying" in layers associated with the presence of hygroscopic ash in the atmosphere that has already been reported in similar episodes after volcanic eruptions in other parts of the world. This particular footprint can be used to detect the probable existence of volcanic ash layers. This study also illustrates the utility of ceilometers to detect not only cloud base at airports but also volcanic ash plumes at the boundary layer and up to 7 km altitude. Aerosol properties measured in the city during the resuspension episode indicate the presence of enhanced concentrations of aerosol particles in the boundary layer along with spectral signatures in the measurements at the Buenos Aires AERONET site typical of ash plus dust advected towards the city. The mandatory aviation reports from the National Weather Service about airborne and deposited volcanic ash at the airport near the measurement site (Aeroparque) correlate in time with the enhanced concentrations. The presence of the resuspended material was detected by the CALIOP lidar overpassing the region. Since the dynamics of ash resuspension and

  2. Long-term variability of heat waves in Argentina and recurrence probability of the severe 2008 heat wave in Buenos Aires

    NASA Astrophysics Data System (ADS)

    Rusticucci, Matilde; Almeira, Gustavo; Pecho, Jozef; Kysely, Jan

    2013-04-01

    Heat waves are one of the main concerns related to the impacts of climate change because their frequency and severity are projected to increase in all projections of future climate. The objective of this work is to study the long-term variability in the occurrence of heat waves over Argentina. The number of days in heat waves per decade is analysed, considering spells of minimum temperature above the 90th percentile (MinTHW), maximum temperature above the 90th percentile (MaxTHW) and spells of days with both minimum and maximum temperature above the corresponding 90th percentile (EHW) for the October-March period. Decadal values in Buenos Aires experienced increases in all definitions of heat waves, but at other stations, the combination of different trends or decadal variability result in some cases in a decrease of extreme heat waves, as shown in Córdoba (central Argentina) and Las Lomitas (northern Argentina). In the northwestern part of the country, La Quiaca and Tinogasta show a strong change in the last decade, mainly due to the increment in the persistence of extreme MinTHW but also accompanied by increases in MaxTHW. In general, other stations show a clear positive trend in MinTHW and decadal variability in MaxTHW, with the largest EHW cases in the last decade. We also estimate recurrence probability of the longest and most severe heat wave in Buenos Aires (over 1909-2010, according to MaxTHW) that occurred from 3 to 14 November 2008. We use simulations with a stochastic autoregressive model that reproduces structure of the time series of daily maximum temperatures in Buenos Aires for (i) the recent climate and (ii) under several scenarios of possible future climate development based on climate models' projections. It is shown that the recurrence probability of such long and severe heat wave is likely to decline substantially in the near future even under a moderate warming trend.

  3. [Histoplasmosis outbreak in Morón, Buenos Aires Province, Argentina].

    PubMed

    Negroni, R; Duré, R; Ortiz Nareto, A; Arechavala, A I; Maiolo, E I; Santiso, G M; Iovannitti, C; Ibarra-Camou, B; Canteros, C E

    2010-01-01

    A histoplasmosis outbreak affecting 6 previously healthy Air Force cadets is herein presented. The patients suffered from fever and respiratory symptoms after having cleaned an abandoned hangar soiled with pigeons and bat droppings. They all presented fever, myalgia, tachypnea, and nonproductive cough. Chest X-ray and CT scan studies showed disseminated reticulonodular images affecting both lungs. Hiliar adenomegalies were also observed. All patients achieved a favourable outcome without antifungal treatment. Both serologic tests searching for specificic antibodies (immunodiffusion and counterimmunoelectrophoresis) and histoplasmin skin tests were positive in all cases. Five soil samples mixed with pigeons and bat droppings were collected from the hangar. Suspensions of these samples were inoculated into 20 hamsters by intraperitoneal injection; mycelial phase of H. capsulatum was isolated from liver and spleen cultures. The genetic profile of this strain was compared with 12 isolates obtained from Argentinean patients, and a great degree of homogeneity was observed (> 96% similarity). Although histoplasmosis is endemic in the wet Pampas, this is the first epidemic outbreak reported south of the 34th parallel.

  4. [Histoplasmosis outbreak in Morón, Buenos Aires Province, Argentina].

    PubMed

    Negroni, R; Duré, R; Ortiz Nareto, A; Arechavala, A I; Maiolo, E I; Santiso, G M; Iovannitti, C; Ibarra-Camou, B; Canteros, C E

    2010-01-01

    A histoplasmosis outbreak affecting 6 previously healthy Air Force cadets is herein presented. The patients suffered from fever and respiratory symptoms after having cleaned an abandoned hangar soiled with pigeons and bat droppings. They all presented fever, myalgia, tachypnea, and nonproductive cough. Chest X-ray and CT scan studies showed disseminated reticulonodular images affecting both lungs. Hiliar adenomegalies were also observed. All patients achieved a favourable outcome without antifungal treatment. Both serologic tests searching for specificic antibodies (immunodiffusion and counterimmunoelectrophoresis) and histoplasmin skin tests were positive in all cases. Five soil samples mixed with pigeons and bat droppings were collected from the hangar. Suspensions of these samples were inoculated into 20 hamsters by intraperitoneal injection; mycelial phase of H. capsulatum was isolated from liver and spleen cultures. The genetic profile of this strain was compared with 12 isolates obtained from Argentinean patients, and a great degree of homogeneity was observed (> 96% similarity). Although histoplasmosis is endemic in the wet Pampas, this is the first epidemic outbreak reported south of the 34th parallel. PMID:21229193

  5. Characterization and health risk assessment of VOCs in occupational environments in Buenos Aires, Argentina

    NASA Astrophysics Data System (ADS)

    Colman Lerner, J. E.; Sanchez, E. Y.; Sambeth, J. E.; Porta, A. A.

    2012-08-01

    To detect volatile organic compounds (VOCs) in indoor air in small enterprises in La Plata city and surrounding areas, sampling was conducted using passive diffusion monitors (3M-3500) and analysis of the samples were performed byCG-FID. Analytic methodology was optimized for 23 VOCs (n-alkanes, cycloalkanes, aromatic and chlorinated compounds, ketones and terpenes compounds) by determining the recovery factor and detection limit for each analyte. Different recovery values were obtained by desorbing with a mixture of dichloromethane: methanol (50:50), with a standard deviation lower than 5%. Enterprise analyzed included chemical analysis laboratories, sewing workrooms, electromechanical repair and car painting centers, take away food shops, and a photocopy center. The highest levels of VOCs were found to be in electromechanical repair and car painting centers (hexane, BTEX, CHCl3, CCl4) followed by chemical analysis laboratories and sewing workrooms. Cancer and noncancer risks were assessed using conventional approaches (HQ and LCR, US EPA) using the benzene, trichloroethylene, chloroform for cancer risk, and toluene, xylene and n-hexane, for noncancer risks as markers. The results showed different LCR for benzene and trichloroethylene between the different indoor environments analyzed (electromechanical repair and car painting center ≫ others) and chloroform (laboratory > others), but comparing with the results obtained by other research, are in similar order of magnitude for equivalents activities. Similar finding were founded for HQ. Comparing these results with the worker protection legislation the electromechanical repair and car painting center and chemical analysis laboratories are close to the limits advised by OSHA and ACGIH. These facts show the importance of the use of abatement technologies for the complete reduction of VOCs levels, to mitigate their impact in the worker's health and their venting to the atmosphere.

  6. Incidence and prevalence of lupus in Buenos Aires, Argentina: a 11-year health management organisation-based study

    PubMed Central

    Scolnik, M; Marin, J; Valeiras, S M; Marchese, M F; Talani, A S; Avellaneda, N L; Etchepare, A; Etchepare, P; Plou, M S; Soriano, E R

    2014-01-01

    Objectives Studies regarding the epidemiology of systemic lupus erythematosus (SLE) are lacking in Argentina. Our purpose was to estimate the incidence and prevalence of SLE in a university hospital-based health management organisation in Buenos Aires (HIMCP). Methods For incidence calculation, the population at risk included all adult members of the HIMCP, with continuous affiliation for at least 1 year from January 1998 to January 2009. Each person was followed until he/she voluntarily left the HIMCP, death or finalisation of the study. Multiple methods for case finding were used to ensure complete ascertainment: (a) patients with problem SLE, undifferentiated autoimmune disease or mixed connective tissue disease in the Computer-based Patient Record System, (b) patients with positive antinuclear antibody test, anti-Sm antibodies and/or anti-dsDNA antibodies in the laboratory database and (c) patients who consumed hydroxichloroquine, chloroquine, azathioprine, cyclophosphamide, mycophenolate, cyclosporine or rituximab, from the administrative HIMCP drugs database. Medical records of all patients found were reviewed, and only patients fulfilling ACR criteria for SLE were included. Global and gender incidence rate (IR) was calculated. Prevalence was estimated on 1 January 2009, and the denominator population was the number of active members >18 years at that date (n=127 959). Results In the study period, 68 patients developed SLE. The observed IR (per 100 000 person-years, (CI 95%)) was 6.3 (4.9 to 7.7) for total population; 8.9 (CI 6.6 to 11.2) for women and 2.6 (1.2 to 3.9) for men. On 1 January 2009, 75 prevalent cases were identified. Prevalence rates (cases per 100 000 habitants, (CI 95%)) were 58.6 (46.1 to 73.5) for total population; 83.2 (63.9 to 106.4) for women and 23 (CI 11.9 to 40.1) for men. Conclusions SLE incidence and prevalence rates in Argentina are in agreement with those of other studies from different parts of the world. PMID:25379189

  7. [Placental weight percentiles and its relationship with fetal weight according to gestational age in an urban area of Buenos Aires].

    PubMed

    Grandi, Carlos; Roman, Estela; Dipierri, Jose

    2015-01-01

    Antecedentes: El peso placentario (PP) y los índices de su relación con el peso al nacer (PN) (PN/PP, PP/PN) predicen morbi-mortalidad perinatal y resultados alejados de la salud. Objetivos: Calcular percentilos del PP e índices por sexo y edad gestacional correspondientes a 867 RNV de la Maternidad Sardá de Buenos Aires, Argentina y compararlos con referencias internacionales. Material y métodos: Se excluyeron feto muerto, embarazo múltiple, edad gestacional <22 y >42 semanas y PP<100g y >2500g. Características maternas y fetales: edad, educación, tabaco, paridad, diabetes, preeclampsia, corioamnionitis, restricción del crecimiento, malformación congénita y prematurez. Se calcularon estadísticos de resumen y percentilos con el método LMS. Las comparaciones se realizaron con test t-Student, ANOVA y referencias internacionales. Resultados: Edad materna media 24 años, educación 10.1 años, 24.5% primíparas, 12.6% fumadoras, 4.9% presentaron diabetes, 8.7% preeclampsia, 7.9% corioamnionitis y 13.0% restricción del crecimiento fetal. El 55.3% de los RN fueron varones, 51.6% prematuros, 18.9% PEG y 7.1% malformados. El PN y EG promedio fue de 2581g y 35.6 semanas respectivamente. Elevada correlación positiva de la EG con PP y PN/PP y negativa con PP/PN (p%lt;0.001); el peso de la placenta e índices fueron mayores en varones. Se presentan los percentiles de PP, PN/PP y PP/PN. Las diferencias con las referencias oscilaron de 0.46% -13%, 4.91% -12.1% y 5.81% -14% para el PP, PN/PP y PP/PN respectivamente. Conclusiones: los percentilos generados son aplicables en investigaciones sobre la relación de la placenta con resultados perinatales y la salud durante el ciclo vital.

  8. Palynofacial analysis in alkaline soils and paleoenvironmental implications: The Paso Otero 5 archaeological site (Necochea district, Buenos Aires province, Argentina)

    NASA Astrophysics Data System (ADS)

    Grill, S.; Borromei, A.; Martínez, G.; Gutierrez, M. A.; Cornou, M. E.; Olivera, D.

    2007-06-01

    The combination of palynofacial and sedimentological analyses constitutes a valuable method for paleoenvironmental and paleoclimatic reconstructions, especially when fossil pollen information is scarce or absent. This methodology elucidates a late Pleistocene/Holocene sequence at the Paso Otero 5 archaeological site in the middle basin of the Quequén Grande River, Necochea district, Buenos Aires province, Argentina. Although the main factor responsible for the destruction of pollen grains is pH, biochemical and chemical oxidation and mechanical damage contribute to the deterioration as well. The site sequence indicates that extremely arid climatic conditions without vegetation cover prevailed during the late Pleistocene (˜12,000 14C yr BP), after which the climate changed to semiarid conditions associated with a disturbed environment due to strong eolian activity (Palynofacies 1 and 2; pre-10,400 14C yr BP). During the Pleistocene/Holocene transition (Palynofacies 3 and 4; ˜10,400-9400 14C yr BP), loamy facies associated with paleosoils reflected stable conditions and temporary ponds (spring deposits). Similar conditions occurred near the end of early Holocene (Palynofacies 5-9; ˜9400-6600 14C yr BP), whereas sandy and silty facies are associated with the flood margins of streams or rivers in the middle and late Holocene (Palynofacies 10-14; 6600-2500 14C yr BP). The top of the sequence (Palynofacies 15 and 16) consists of alluvium sediments and reflects locally humid conditions and modern vegetation with anthropic influence. One of the earliest Pampean sites with evidence of humans (10,450-10,200 14C yr BP), Paso Otero 5, provides a variety of megafauna bone specimens associated with ``fish-tail" projectile points, a lithic artifact diagnostic of early human occupations in South America. The site contains a complete stratigraphic record from the late Pleistocene to the present. The evidence presented herein supports the hypothesis that human colonization, at

  9. [Mycotrophic capacity and efficiency of microbial consortia of arbuscular mycorrhizal fungi native of soils from Buenos Aires province under contrasting management].

    PubMed

    Thougnon Islas, Andrea J; Eyherabide, Mercedes; Echeverría, Hernán E; Sainz Rozas, Hernán R; Covacevich, Fernanda

    2014-01-01

    We characterized the infective and sporulation capacities of microbial consortia of arbuscular mycorrhizal fungi (AMF) native of Buenos Aires province (Argentina) and determined if some soil characteristics and mycorrhizal parameters could allow to select potentially beneficial inocula. Soil samples were selected from seven locations in Buenos Aires province all under agricultural (A) and pristine (P) conditions. The AMF were multiplied and mycorrhizal root colonization of trap plants was observed at 10 weeks of growth. Spore number in field was low; however, after multiplication spore density accounted for 80-1175 spores per 100g of soil. The principal component analysis showed that the P and Fe soil contents are the main modulators of infectivity and sporulation capacity. The mycorrhizal potential was determined in three locations, being high in Pristine Lobería and Agricultural Trenque Lauquen and low in Junín. Agricultural Lobería (AL) and Pristine Lobería (PL) inocula were selected and their efficiency was evaluated under controlled conditions. Even though shoot dry matter increases after inoculation was not significant (p>0.05) mycorrhizal response was greater than 40% for tomato and 25% for corn, particularly after inoculation with inocula from the agricultural management. These results could be associated to the incipient development of mycorrhizae in both species. Additional research should be conducted to further develop our findings in order to determine the factors involved in the selection of efficient inocula. PMID:25011598

  10. [Endemic goiter in Argentina. Epidemiologic studies before (1968) and during (1986) prophylaxis with iodized salt in the city of Buenos Aires].

    PubMed

    Salvaneschi, J P; Salvaneschi, B G; Moralejo, A B; García, J R

    1991-01-01

    An epidemiological survey on goiter was conducted in Buenos Aires, on 3882 schoolchildren, a randomized sample of 160,026 children of public schools, before establishing prophylactic measures in 1968. With an estimated error of 5% the result was 14.8% of goiter prevalence. This figure qualified Buenos Aires as an endemic area and was, therefore, included in the National Programme to Combat Endemic Goiter, through iodized salt (proportion 1:30,000 of salt). Schools were qualified according to their social level in high, middle and low; the prevalence of goiter was 6.8%, 12.2% and 26.4%, respectively. The prevalence was also higher in older ages. In 1986, using the same randomized sample, a second survey was conducted, after 18 years of prophylaxis. Goiter prevalence was 8.5%. Goiter in the low social class was about the same as in the middle class. In randomized samples of 49 urines, iodine concentration was determined yielding an average of 139.6 micrograms/g creatinine (median 120.9 micrograms/g creatinine). Urinary iodine level in 14 children with goiter was 146.1 micrograms/g creatinine and in 12 without goiter was 171.4 micrograms/g. There was no statistical difference between these three groups. Variations in radioiodine uptake, urinary iodine, plasma inorganic iodine levels, and water iodine are discussed.

  11. NMVOCs speciated emissions from mobile sources and their effect on air quality and human health in the metropolitan area of Buenos Aires, Argentina

    NASA Astrophysics Data System (ADS)

    D'Angiola, Ariela; Dawidowski, Laura; Gomez, Dario; Granier, Claire

    2014-05-01

    Since 2007, more than half of the world's population live in urban areas. Urban atmospheres are dominated by pollutants associated with vehicular emissions. Transport emissions are an important source of non-methane volatile organic compounds (NMVOCs) emissions, species of high interest because of their negative health effects and their contribution to the formation of secondary pollutants responsible for photochemical smog. NMVOCs emissions are generally not very well represented in emission inventories and their speciation presents a high level of uncertainty. In general, emissions from South American countries are still quite unknown for the international community, and usually present a high degree of uncertainty due to the lack of available data to compile emission inventories. Within the Inter-American Institute for Global Change Research (IAI, www.iai.int) projects, UMESAM (Urban Mobile Emissions in South American Megacities) and SAEMC (South American Emissions, Megacities and Climate, http://saemc.cmm.uchile.cl/), the effort was made to compute on-road transport emission inventories for South American megacities, namely Bogota, Buenos Aires, Lima, Sao Paulo and Santiago de Chile, considering megacities as urban agglomerations with more than 5 million inhabitants. The present work is a continuation of these projects, with the aim to extend the calculated NMVOCs emissions inventory into the individual species required by CTMs. The on-road mobile sector of the metropolitan area of Buenos Aires (MABA), Argentina, accounted for 70 Gg of NMVOCs emissions for 2006, without considering two-wheelers. Gasoline light-duty vehicles were responsible for 64% of NMVOCs emissions, followed by compressed natural gas (CNG) light-duty vehicles (22%), diesel heavy-duty vehicles (11%) and diesel light-duty vehicles (7%). NMVOCs emissions were speciated according to fuel and technology, employing the European COPERT (Ntziachristos & Samaras, 2000) VOCs speciation scheme for

  12. Long-term variability of heat waves in Argentina and recurrence probability of the severe 2008 heat wave in Buenos Aires

    NASA Astrophysics Data System (ADS)

    Rusticucci, Matilde; Kyselý, Jan; Almeira, Gustavo; Lhotka, Ondřej

    2016-05-01

    Heat waves are one of the main concerns related to the impacts of climate change, because their frequency and severity are projected to increase in a future climate. The objectives of this work are to study the long-term variability of heat waves over Argentina and to estimate recurrence probability of the most severe 2008 heat wave in Buenos Aires. We used three definitions of heat waves that were based on (1) daily maximum temperature above the 90th percentile (MaxTHW), (2) daily minimum temperature above the 90th percentile (MinTHW) and (3) both maximum and minimum temperatures above the corresponding 90th percentiles (EHW). The minimum length of a heat wave was 3 days, and the analysis was performed over the October-March period. Decadal values in Buenos Aires experienced clear increases in heat waves according to MinTHW and EHW, with the highest frequency for both in the 2001-2010 decade, but at other stations, combinations of different trends and decadal variability resulted in some cases in a decrease of extreme heat waves. In the north-western part of the country, a strong positive change in the last decade was found, mainly due to the increment in the persistence of MinTHW but also accompanied by increases in MaxTHW. In general, other stations show a clear positive trend in MinTHW and decadal variability in MaxTHW, with the largest EHW cases in the last decade. We also estimated recurrence probability of the longest and most severe heat wave in Buenos Aires (over 1909-2010, according to intensity measured by the cumulative excess of maximum daily temperature above the 90th percentile) that occurred from 3 to 14 November 2008, by means of simulations with a stochastic first-order autoregressive model. The recurrence probability of such long and severe heat wave is small in the present climate but it is likely to increase substantially in the near future even under a moderate warming trend.

  13. [The Universidad de Buenos Aires in the 1970s: analysis of the press release expressing solidarity with Rodolfo Puiggrós].

    PubMed

    Recalde, Aritz

    2012-12-01

    This article reconstructs the historical context surrounding the emission of a press release by authorities of the Universidad de Buenos Aires, expressing their solidarity with historian and university rector Rodolfo Puiggrós after his resignation. In his term, Puiggrós attempted to fuse the institution's scientific and technological plans with the new development model initiated by Héctor Cámpora and the Peronist movement in 1973. The article makes reference to the project of university transformation promoted by Puiggrós, combining the ideological traditions of Marxism, reformism, and nationalism. A few key points, useful for analyzing the reasons behind Puiggrós's resignation, are presented, and the arguments highlighted within this press release, published by Office of Media and Communications on October 5, 1973, are discussed.

  14. [New documental evidence on the history of homeopathy in Latin America: a case study of links between Rio de Janeiro and Buenos Aires].

    PubMed

    Tarcitano, Conrado Mariano; Waisse, Silvia

    2016-01-01

    Homeopathy began to spread soon after it was formulated by Samuel Hahnemann in the early 1800s, reaching the Southern Cone in the 1830s. In processes of this kind, one figure is often cited as being responsible for introducing it, often attaining quasi-mythical status. Little is known, however, about how homeopathy reached Argentina at that time. Through archival research, we discovered that medical and lay homeopaths circulated between Rio de Janeiro and Buenos Aires. Given the well-known proselytizing of the circles gravitating around lay homeopaths B. Mure and J.V. Martins in Rio de Janeiro, the documents indicate that this movement actually went as far as Argentina, which had not been confirmed until now. PMID:27438734

  15. [The Universidad de Buenos Aires in the 1970s: analysis of the press release expressing solidarity with Rodolfo Puiggrós].

    PubMed

    Recalde, Aritz

    2012-12-01

    This article reconstructs the historical context surrounding the emission of a press release by authorities of the Universidad de Buenos Aires, expressing their solidarity with historian and university rector Rodolfo Puiggrós after his resignation. In his term, Puiggrós attempted to fuse the institution's scientific and technological plans with the new development model initiated by Héctor Cámpora and the Peronist movement in 1973. The article makes reference to the project of university transformation promoted by Puiggrós, combining the ideological traditions of Marxism, reformism, and nationalism. A few key points, useful for analyzing the reasons behind Puiggrós's resignation, are presented, and the arguments highlighted within this press release, published by Office of Media and Communications on October 5, 1973, are discussed. PMID:23681463

  16. High Prevalence of Substance Use among Men who have Sex with Men in Buenos Aires, Argentina: Implications for HIV Risk Behavior

    PubMed Central

    Balán, Iván C.; Carballo-Diéguez, Alex; Dolezal, Curtis; Marone, Rubén; Pando, María A.; Barreda, Victoria; Ávila, María M

    2012-01-01

    Five hundred gay and other men who have sex with men (G&MSM) from Buenos Aires, Argentina completed an assessment regarding substance use and sexual behavior. During the past two months, 78% of participants consumed alcohol and 61% drugs. Over 20% of participants reporting alcohol, marijuana, cocaine sulfate, or tranquilizer use, did so daily. Heavy alcohol use was more likely among participants with greater mood reactivity (AOR = 1.64) and less likely among those who identified as gay (AOR=0.38). Weekly drug use was less likely among older (AOR=0.98), and gay-identified participants (AOR=0.50), but more likely among participants with greater mood reactivity (AOR=1.49). Drug use was correlated with unprotected anal and vaginal intercourse with men, women, and transvestites among non-gay identified participants (r= 0.22). Findings highlight the need to reduce substance use and sexual risk behavior in this population. PMID:23196860

  17. [The origin and quality of water for human consumption: the health of the population residing in the Matanza-Riachuelo river basin area in Greater Buenos Aires].

    PubMed

    Monteverde, Malena; Cipponeri, Marcos; Angelaccio, Carlos; Gianuzzi, Leda

    2013-04-01

    The aim of this study is to analyze the origin and quality of water used for consumption in a sample of households in Matanza-Riachuelo river basin area in Greater Buenos Aires, Argentina. The results of drinking water by source indicated that 9% of water samples from the public water system, 45% of bottled water samples and 80% of well water samples were not safe for drinking due to excess content of coliforms, Escherichia coli or nitrates. Individuals living in households where well water is the main source of drinking water have a 55% higher chance of suffering a water-borne disease; in the cases of diarrheas, the probability is 87% higher and in the case of dermatitis, 160% higher. The water for human consumption in this region should be provided by centralized sources that assure control over the quality of the water.

  18. Hydrogeochemical and isotopic characterisation of groundwater in a sand-dune phreatic aquifer on the northeastern coast of the province of Buenos Aires, Argentina.

    PubMed

    Carretero, Silvina C; Dapeña, Cristina; Kruse, Eduardo E

    2013-01-01

    This contribution presents the hydrochemical and isotopic characterisation of the phreatic aquifer located in the Partido de la Costa, province of Buenos Aires, Argentina. In the sand-dune barrier geomorphological environment, groundwater is mainly a low-salinity Ca-HCO3 and Na-HCO3-type, being in general suitable for drinking, whereas in the continental plain (silty clay sediments), groundwater is a Na-Cl type with high salinity and unsuitable for human consumption. The general isotopic composition of the area ranges from-6.8 to-4.3 ‰ for δ(18)O and from-39 to-21 ‰ for δ(2)H, showing that rainwater rapidly infiltrates into the sandy substrate and reaches the water table almost without significant modification in its isotopic composition. These analyses, combined with other chemical parameters, made it possible to corroborate that in the eastern area of the phreatic aquifer, there is no contamination from marine salt water.

  19. Prospective Multicenter Study of Community-Associated Skin and Skin Structure Infections due to Methicillin-Resistant Staphylococcus aureus in Buenos Aires, Argentina

    PubMed Central

    López Furst, María José; de Vedia, Lautaro; Fernández, Silvina; Gardella, Noella; Ganaha, María Cristina; Prieto, Sergio; Carbone, Edith; Lista, Nicolás; Rotryng, Flavio; Morera, Graciana I.; Mollerach, Marta; Stryjewski, Martín E.

    2013-01-01

    Background Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is now the most common cause of skin and skin structure infections (SSSI) in several world regions. In Argentina prospective, multicenter clinical studies have only been conducted in pediatric populations. Objective Primary: describe the prevalence, clinical and demographic characteristics of adult patients with community acquired SSSI due to MRSA; secondary: molecular evaluation of CA-MRSA strains. Patients with MRSA were compared to those without MRSA. Materials and Methods Prospective, observational, multicenter, epidemiologic study, with molecular analysis, conducted at 19 sites in Argentina (18 in Buenos Aires) between March 2010 and October 2011. Patients were included if they were ≥14 years, were diagnosed with SSSI, a culture was obtained, and there had no significant healthcare contact identified. A logistic regression model was used to identify factors associated with CA-MRSA. Pulse field types, SCCmec, and PVL status were also determined. Results A total of 311 patients were included. CA-MRSA was isolated in 70% (218/311) of patients. Clinical variables independently associated with CA-MRSA were: presence of purulent lesion (OR 3.29; 95%CI 1.67, 6.49) and age <50 years (OR 2.39; 95%CI 1.22, 4.70). The vast majority of CA-MRSA strains causing SSSI carried PVL genes (95%) and were SCCmec type IV. The sequence type CA-MRSA ST30 spa t019 was the predominant clone. Conclusions CA-MRSA is now the most common cause of SSSI in our adult patients without healthcare contact. ST30, SCCmec IV, PVL+, spa t019 is the predominant clone in Buenos Aires, Argentina. PMID:24324543

  20. Children’s palliative care now! Highlights from the second ICPCN conference on children’s palliative care, 18–21 May 2016, Buenos Aires, Argentina

    PubMed Central

    Downing, J; Kiman, R; Boucher, S; Nkosi, B; Steel, B; Marston, C; Lascar, E; Marston, J

    2016-01-01

    The International Children’s Palliative Care Network held its second international conference on children’s palliative care in Buenos Aires, Argentina, from the 18th–21st May 2016. The theme of the conference was ‘Children’s Palliative Care…. Now!’ emphasising the need for palliative care for children now, as the future will be too late for many of them. Six pre-conference workshops were held, addressing issues connected to pain assessment and management, adolescent palliative care, ethics and decision-making, developing programmes, the basics of children’s palliative care, and hidden aspects of children’s palliative care. The conference brought together 410 participants from 40 countries. Plenary, concurrent, and poster presentations covered issues around the status of children’s palliative care, genetics, perinatal and neonatal palliative care, the impact of children’s palliative care and the experiences of parents and volunteers, palliative care as a human right, education in children’s palliative care, managing complex pain in children, spiritual care and when to initiate palliative care. The ‘Big Debate’ explored issues around decision-making and end of life care in children, and gave participants the opportunity to explore a sensitive and thought provoking topic. At the end of the conference, delegates were urged to sign the Commitment of Buenos Aires which called for governments to implement the WHA resolution and ensure access to palliative care for neonates, children and their families, and also commits us as palliative care providers to share all that we can and collaborate with each other to achieve the global vision of palliative care for all children who need it. The conference highlighted the ongoing issues in children’s palliative care and participants were continually challenged to ensure that children can access palliative care NOW.

  1. Children’s palliative care now! Highlights from the second ICPCN conference on children’s palliative care, 18–21 May 2016, Buenos Aires, Argentina

    PubMed Central

    Downing, J; Kiman, R; Boucher, S; Nkosi, B; Steel, B; Marston, C; Lascar, E; Marston, J

    2016-01-01

    The International Children’s Palliative Care Network held its second international conference on children’s palliative care in Buenos Aires, Argentina, from the 18th–21st May 2016. The theme of the conference was ‘Children’s Palliative Care…. Now!’ emphasising the need for palliative care for children now, as the future will be too late for many of them. Six pre-conference workshops were held, addressing issues connected to pain assessment and management, adolescent palliative care, ethics and decision-making, developing programmes, the basics of children’s palliative care, and hidden aspects of children’s palliative care. The conference brought together 410 participants from 40 countries. Plenary, concurrent, and poster presentations covered issues around the status of children’s palliative care, genetics, perinatal and neonatal palliative care, the impact of children’s palliative care and the experiences of parents and volunteers, palliative care as a human right, education in children’s palliative care, managing complex pain in children, spiritual care and when to initiate palliative care. The ‘Big Debate’ explored issues around decision-making and end of life care in children, and gave participants the opportunity to explore a sensitive and thought provoking topic. At the end of the conference, delegates were urged to sign the Commitment of Buenos Aires which called for governments to implement the WHA resolution and ensure access to palliative care for neonates, children and their families, and also commits us as palliative care providers to share all that we can and collaborate with each other to achieve the global vision of palliative care for all children who need it. The conference highlighted the ongoing issues in children’s palliative care and participants were continually challenged to ensure that children can access palliative care NOW. PMID:27610193

  2. Children's palliative care now! Highlights from the second ICPCN conference on children's palliative care, 18-21 May 2016, Buenos Aires, Argentina.

    PubMed

    Downing, J; Kiman, R; Boucher, S; Nkosi, B; Steel, B; Marston, C; Lascar, E; Marston, J

    2016-01-01

    The International Children's Palliative Care Network held its second international conference on children's palliative care in Buenos Aires, Argentina, from the 18th-21st May 2016. The theme of the conference was 'Children's Palliative Care…. Now!' emphasising the need for palliative care for children now, as the future will be too late for many of them. Six pre-conference workshops were held, addressing issues connected to pain assessment and management, adolescent palliative care, ethics and decision-making, developing programmes, the basics of children's palliative care, and hidden aspects of children's palliative care. The conference brought together 410 participants from 40 countries. Plenary, concurrent, and poster presentations covered issues around the status of children's palliative care, genetics, perinatal and neonatal palliative care, the impact of children's palliative care and the experiences of parents and volunteers, palliative care as a human right, education in children's palliative care, managing complex pain in children, spiritual care and when to initiate palliative care. The 'Big Debate' explored issues around decision-making and end of life care in children, and gave participants the opportunity to explore a sensitive and thought provoking topic. At the end of the conference, delegates were urged to sign the Commitment of Buenos Aires which called for governments to implement the WHA resolution and ensure access to palliative care for neonates, children and their families, and also commits us as palliative care providers to share all that we can and collaborate with each other to achieve the global vision of palliative care for all children who need it. The conference highlighted the ongoing issues in children's palliative care and participants were continually challenged to ensure that children can access palliative care NOW. PMID:27610193

  3. Quality of life in type 2 diabetes mellitus patients requiring insulin treatment in Buenos Aires, Argentina: a cross-sectional study

    PubMed Central

    Pichon-Riviere, Andres; Irazola, Vilma; Beratarrechea, Andrea; Alcaraz, Andrea; Carrara, Carolina

    2015-01-01

    Background: Decision-makers have begun to recognize Health-Related Quality of Life (HRQoL) as an important and measurable outcome of healthcare interventions; and HRQoL data is increasingly being used by policy-makers to prioritize health resources. Our objective was to measure HRQoL in a group of Type 2 Diabetes Mellitus (T2DM) patients receiving insulin treatment in Buenos Aires, Argentina. Methods: We conducted a cross-sectional study of patients with T2DM over 21 years of age, treated with either Neutral Protamine Hagedorn (NPH) insulin or Insulin Glargine (IG), who had not changed their baseline schedule in the last 6 months. The recruitment was during 2006–7 in nine private diabetes specialists’ offices in Buenos Aires, Argentina. A standardized diabetes-specific HRQoL questionnaire, the Audit of Diabetes Dependent Quality of Life (ADDQoL), was used. Results: A total of 183 patients were included (93 receiving NPH and 90 receiving IG). The mean QoL score was: 0.98 (SD: 0.89) and the diabetes specific QoL was: -1.49 (SD: 0.90). T2DM had a negative impact on HRQoL with a mean Average Weighted Impact (AWI) score on QoL of -1.77 (SD: 1.58). The greatest negative impact was observed for domains: ‘worries about the future’, ‘freedom to eat’, ‘living conditions’, ‘sex life’, and ‘family life’. The mean AWI score was -1.71 (SD: 1.48) in patients treated with IG and -1.85 (SD: 1.68) in patients receiving NPH, this difference was not statistically significant. Conclusion: The ADDQoL questionnaire is a tool that can be used in Argentina to measure the QoL of patients with diabetes when evaluating diabetes care programs. The scores of QoL in our selected population did not differ from those reported in high-income countries. We expect that the results of this study will increase healthcare providers’ awareness of patients’ perceived QoL and help to overcome the barriers that delay insulin treatment; mainly clinical inertia and patient

  4. Nutritional Biochemistry

    NASA Technical Reports Server (NTRS)

    Smith, Scott M.

    2010-01-01

    This slide presentation reviews some of the effects that space flight has on humans nutritional biochemistry. Particular attention is devoted to the study of protein breakdown, inflammation, hypercatabolism, omega 3 fatty acids, vitamin D, calcium, urine, folate and nutrient stability of certain vitamins, the fluid shift and renal stone risk, acidosis, iron/hematology, and the effects on bone of dietary protein, potassium. inflammation, and omega-3 fatty acids

  5. [Peers contribution to mental health work: considerations on the relationship between formal and informal help. A case study in a mental health facility in Buenos Aires].

    PubMed

    Ardila Gómez, Sara; Agrest, Martín; Abadi, Daniel; Cáceres, Carmen

    2013-01-01

    Including peers to the mental health workforce has been part of a larger debate regarding specificity and incumbencies of peers' work, as well as their relationship with other workers. While some members of the workforce are proposing to train peers to have them help in different settings and interventions, others see them as unfair and underprepared competitors trying to replace them. Based on this debate, and from a Collective Health perspective on the concept of "care", this paper supports the idea that, since they put "care" as central to anything done by the mental health workforce, peers could be crucial to practices in Mental Health. An experience that took place in a Day Hospital in Buenos Aires City, conversed within a group dedicated to reflect on peers' support, is analyzed in order to unveil the relationship between peers and professionals, as well as peers' specificity. It is withstand that one of the main contributions of including peers is to help professionals think more about the relational dimension of their work and about the way they treat, call and refer to patients.

  6. ["Knowing about AIDS" and sexual precautions among low-income women from the southern area of Buenos Aires. Notes for defining prevention policies].

    PubMed

    Grimberg, M

    2001-01-01

    This study is part of a line of research on gender and prevention in a research program on the social construction of HIV/AIDS. We present the results of an ethnographic study among low-income women 15-35 years old in the southern area of Buenos Aires. The area has the highest number of HIV/AIDS cases and high poverty levels, extensive social degradation, and urban violence. According to our results, in the interface between "knowing about" and "behaving" there are complex processes involving stigmatized and gender-biased representations of HIV/AIDS as "other people's problem" and social and sexual relations permeated by gender stereotypes and roles. We believe that planning of prevention should be based on the consideration of overall social practices and specifically the characteristics of gender relations, prioritizing relational strategies between women and men and promoting critical reflection on the main nodes organizing daily life and active participation in the production of social relations and practices of reciprocity and equity. The increasingly precarious conditions in social life intensifies poor women's vulnerability and social interaction contexts that relate to the socioeconomic and symbolic role played by women.

  7. [The frontiers of medicalization: tensions surrounding the identification and appreciation of child malnutrition in a primary healthcare center of the city of Buenos Aires].

    PubMed

    Herkovits, Damián

    2012-09-01

    The medicalization of life and its implications for the production of subjectivities are phenomena that have been highlighted by the human sciences in the study of health and disease. Nevertheless, the analysis of its local expressions has been insufficiently covered. The scope of this paper is to explore this field by an ethnographical study of the medicalization process of child malnutrition in a primary healthcare center of the city of Buenos Aires. We will describe analytically the singularities involved in the body perception and the alimentary context by health professionals and their patients. We emphasize that the criteria of perception and moral values that encourage social positions of health professionals and recipients of their actions precluded the institutionalization of a medical vision. We conclude that the process analyzed highlights the need to exceed the medicalization approaches dealing exclusively from the angle of imposition. The social history of the groups involved and ways of establishing relationships in local settings, are essential to understand the peculiarities of these processes.

  8. Phylogenetic and molecular analyses of human parainfluenza type 3 virus in Buenos Aires, Argentina, between 2009 and 2013: The emergence of new genetic lineages.

    PubMed

    Goya, Stephanie; Mistchenko, Alicia Susana; Viegas, Mariana

    2016-04-01

    Despite that human parainfluenza type 3 viruses (HPIV3) are one of the leading causes of acute lower respiratory tract infections in children under five, there is no licensed vaccine and there is limited current information on the molecular characteristics of regional and global circulating strains. The aim of this study was to describe the molecular characterization of HPIV3 circulating in Buenos Aires. We performed a genetic and phylogenetic analysis of the HN glycoprotein gene. Between 2009 and 2013, 124 HPIV3-positive samples taken from hospitalized pediatric patients were analyzed. Four new genetic lineages were described. Among them, C1c and C3d lineages showed local circulation patterns, whereas C3e and C3f comprised sequences from very distant countries. Despite the diversity of the described genotypes, C3a and C3d predominated over the others, the latter was present during the first years of the study and it was progressively replaced by C3a. Molecular analyses showed 28 non-synonymous substitutions; of these, 13 were located in potentially predicted B-cell epitopes. Taken together, the emergence of genetic lineages and the information of the molecular characteristics of HN protein may contribute to the general knowledge of HPIV3 molecular epidemiology for future vaccine development and antiviral therapies. PMID:26780643

  9. β-lactamases produced by amoxicillin-clavulanate-resistant enterobacteria isolated in Buenos Aires, Argentina: a new blaTEM gene.

    PubMed

    Di Conza, José A; Badaracco, Alejandra; Ayala, Juan; Rodríguez, Cynthia; Famiglietti, Angela; Gutkind, Gabriel O

    2014-01-01

    Resistance to β-lactam/β-lactamase inhibitors in enterobacteria is a growing problem that has not been intensively studied in Argentina. In the present work, 54/843 enterobacteria collected in a teaching hospital of Buenos Aires city were ampicillin-sulbactam-resistant isolates remaining susceptible to second- and third-generation cephalosporins. The enzymatic mechanisms present in the isolates, which were also amoxicillin-clavulanic acid (AMC)-resistant (18/54) were herein analyzed. Sequencing revealed two different variants of blaTEM-1, being blaTEM-1b the most frequently detected allelle (10 Escherichia coli, 3 Klebsiella pneumoniae, 2 Proteus mirabilis and 1 Raoultella terrigena) followed by blaTEM-1a (1 K. pneumoniae). Amoxicillin-clavulanate resistance seems to be mainly associated with TEM-1 overproduction (mostly in E. coli) or co-expressed with OXA-2-like and/or SHV β-lactamases (K. pneumoniae and P. mirabilis). A new blaTEM variant (TEM-163) was described in an E. coli strain having an AMC MIC value of 16/8μg/ml. TEM-163 contains Arg275Gln and His289Leu amino acid substitutions. On the basis of the high specific activity and low IC50 for clavulanic acid observed, the resistance pattern seems to be due to overproduction of the new variant of broad spectrum β-lactamase rather than to an inhibitor-resistant TEM (IRT)-like behavior.

  10. Histology and gametogenesis in Heleobia piscium (Cochliopidae) from the Multiple Use Reserve “Isla Martín García,” Buenos Aires, Argentina

    PubMed Central

    Díaz, Ana C.

    2016-01-01

    Heleobia piscium (d’Orbigny, 1835), a member of the Cochliopidae family found only in South America, is distributed from Entre Ríos, Delta del Paraná, and the littoral of the Río de la Plata down as far as to Punta Indio (Buenos Aires), the southernmost limit of the snail’s geographical distribution. To date, little information is available regarding the reproductive cycle of species within this family either in Argentina or throughout South America. The present work analyzed the histology of the reproductive system of the gonochoric species H. piscium and determined the stages oogenesis and spermatogenesis under natural conditions. Specimens of H. piscium were collected in the Multiple-Use Natural Reserve Isla Martín García, located in the Upper Río de la Plata estuary to the south of the mouth of the Uruguay River. The gametogenic cycle in both sexes was found to consist of the following stages: early maturation, maturation, and evacuation. The maturation period was found to extend from January to October and evacuation of the gametes to start in November and end in February (summer in the Southern Hemisphere). The results indicated the H. piscium exhibit a reproductive cycle without a resting period. PMID:27761336

  11. Relationship between precipitation and water-table fluctuation in a coastal dune aquifer: northeastern coast of the Buenos Aires province, Argentina

    NASA Astrophysics Data System (ADS)

    Carretero, Silvina C.; Kruse, Eduardo E.

    2012-12-01

    The water-table fluctuation (WTF) method is one of the most widely used means to estimate aquifer recharge. In the northeastern coast of the Buenos Aires province, Argentina, the geomorphological and climatic characteristics, as well as the presence of a shallow, homogeneous unconfined aquifer, make it possible to apply this methodology. The relationship between water-table fluctuations and precipitation in a humid climate, considering its seasonal variations, is assessed. Water tables were measured monthly between February 2008 and September 2010 in a monitoring network; rainfall data were analysed. The water table rises when the accumulated precipitation between measurements is more than 53 mm/month in the dry season and more than 97 mm/month in the rainy season. The index, relating water-table fluctuations and precipitation occurring between measurements, shows that values below 0 suggest no increase in the water reserves, whereas higher values entail an increase. In the study area, where there is a lack of historical data, finding out the relationship between water-table fluctuations and precipitation will constitute a tool for groundwater use and management, and set up an early warning system for dry periods. It could also be extrapolated to other regions with similar hydrological conditions lacking in data.

  12. A Series of Stacked and Entrenched Fan Deltas at Lake General Carrera/Buenos Aires (Chile/Argentina) as Terrestrial Analog to Understand the Entrenchment of Martian Deltas

    NASA Astrophysics Data System (ADS)

    Di Achille, G.; Salese, F.

    2014-12-01

    The apparent lack of entrenchment on Martian fan deltas has been hitherto uniquely interpreted as evidence for fast disappearance of water and used to support the hypothesis of an abrupt climate change on Mars around 3.7-3.4 Ga. However, hi-res imagery and topography of several martian fan deltas show evidence of erosion and entrenchment in response to decreasing water level settings. Moreover, the boundary conditions for entrenchment have never been investigated from a quantitative point of view. To gain a quantitative understanding on fan delta entrenchment processes, we are studying (using remote sensing, modeling, and fieldwork campaigns) a series of seven stacked fan deltas formed at Lake General Carrera/Buenos Aires (Chile/Argentina). The uppermost deposit is located more than 400 m above the lowest delta presently forming at the current lake water level. The six raised deltas formed during highstands punctuating the overall 15000 yrs long retreat of the lake and are entirely entrenched thus allowing potential sampling over the entire thickness of the sedimentary sequences. These windows to the internal outcrops enable the assessment of water and sediment paleo-discharges (based on the analysis of the grain sizes) and to implement numerical simulations to reproduce the sedimentary deposits using numeric code such as Sedflux 2.0 (Hutton and Syvitski, 2008). The studied sedimentary assemblage offers the unique opportunity to place constraints on the fan deltas entrenchment with strong implications for the modeling of martian entrenched fans and thus for the understanding of the paleoclimate and paleohydrology during their terminal activity. We present the results of remote sensing study from satellite hi-res imagery and topography, numerical modeling, and from a survey field campaign. E. W. H. Hutton and J. P. M. Syvitski, Sedflux 2.0: An advanced process-response model that generates three-dimensional stratigraphy, Computers & Geosciences 34 (2008) 1319-1337.

  13. Social and environmental health determinants and their relationship with parasitic diseases in asymptomatic children from a shantytown in Buenos Aires, Argentina

    PubMed Central

    Garbossa, Graciela; Pía Buyayisqui, María; Geffner, Laura; López Arias, Ludmila; de la Fournière, Sofía; Haedo, Ana S; Marconi, Adela E; Frid, Juan C; Nesse, Alcira B; Bordoni, Noemí

    2013-01-01

    Health inequities are a common problem for all countries and are the result of not only adverse social conditions but also poor public policies. Today chronic diseases represent the most relevant threats and are a current challenge. Parasitic infections, a leading cause of child morbidity affecting low-income populations, can be transmitted because of an unhealthy environment. Notwithstanding, scarce data have been published on the epidemiological profile of intestinal parasitoses in asymptomatic children living in shantytowns. Vulnerable populations settled in slums are growing in Argentina, particularly in Buenos Aires city. Consequently, this work intended to screen healthy carriers of enteric parasites and determine the epidemiologic profile in asymptomatic children residing in one of those communities, to explore risk factors associated with the transmission of parasites, and to initiate a basic health education campaign to promote healthy behavior in the community. Fecal samples (n = 138) were analyzed by conventional parasitological methods and a survey gathered data on symptoms, family composition, and environmental and hygiene-related variables. High prevalence of feco-orally-transmitted parasitoses (83.3%) and polyparasitism were remarkable findings. The main environmental health determinants were those related to excreta disposal and water provision. Health promotion actions were performed through the diffusion of a set of posters with iconic images and brief messages for health education. Results suggest the need for an environmental sanitation policy to complement health promotion actions. It is essential to spread the results of investigations that address inequities and social determinants of health in order to integrate data with local political processes and alert on acceptable actions for developing appropriate interventions. PMID:23683369

  14. TENDENCIA DE LA TUBERCULOSIS EN LA REGIÓN SANITARIA V DE LA PROVINCIA DE BUENOS AIRES, AÑOS 2000–2011

    PubMed Central

    CHIRICO, CRISTINA; SANJURJO, MYRIAM; IRIBARREN, SARAH; APPENDINO, ANDREA; ZERBINI, ELSA; ETCHEVARRIA, MIRTA

    2016-01-01

    El objetivo del presente trabajo fue analizar la situación epidemiológica de la tuberculosis (TB) en la Región Sanitaria V (RSV), provincia de Buenos Aires. El estudio de tendencia permitió conocer un valor promedio de las variaciones de la tasa de incidencia (TI), calculadas por regresión lineal simple y expresadas como variación anual promedio (VAP). Se analizaron el número de casos notificados y TI por 100 000 habitantes de todas las formas de TB, los casos de TB pulmonar (TBP) y TBP confirmados por bacteriología, total casos por grupos de edad: 0 – 14; 15 – 29 y mayores de 64 años, entre el 1° de enero de 2000 al 31 de diciembre de 2011. La declinación de la TI fue menor al 5% para todas las formas de TB e inferior en las TBP confirmadas bacteriológicamente. Los casos de TBP y TI más elevadas, se concentraron en el grupo de 15 a 29 años, con tendencia estable o ligeramente ascendente de la TI en la TBP bacilífera. El mismo comportamiento presentaron los casos de TBP infantil con confirmación bacteriológica. La mayor velocidad de descenso en la TI de la TBP se produjo en este grupo de edad, mientras que en mayores de 64 años, el descenso fue sostenido en el tiempo. La TB persiste como un riesgo de salud en la RSV, con casos en edades jóvenes, por lo que sigue siendo necesario fortalecer el control de la TB en esta región. PMID:26117604

  15. Use of Respondent Driven Sampling (RDS) Generates a Very Diverse Sample of Men Who Have Sex with Men (MSM) in Buenos Aires, Argentina

    PubMed Central

    Carballo-Diéguez, Alex; Balan, Ivan; Marone, Rubén; Pando, María A.; Dolezal, Curtis; Barreda, Victoria; Leu, Cheng-Shiun; Ávila, María Mercedes

    2011-01-01

    Background Prior research focusing on men who have sex with men (MSM) conducted in Buenos Aires, Argentina, used convenience samples that included mainly gay identified men. To increase MSM sample representativeness, we used Respondent Driven Sampling (RDS) for the first time in Argentina. Using RDS, under certain specified conditions, the observed estimates for the percentage of the population with a specific trait are asymptotically unbiased. We describe, the diversity of the recruited sample, from the point of view of sexual orientation, and contrast the different subgroups in terms of their HIV sexual risk behavior. Methodology 500 MSM were recruited using RDS. Behavioral data were collected through face-to-face interviews and Web-based CASI. Conclusion In contrast with prior studies, RDS generated a very diverse sample of MSM from a sexual identity perspective. Only 24.5% of participants identified as gay; 36.2% identified as bisexual, 21.9% as heterosexual, and 17.4% were grouped as “other.” Gay and non-gay identified MSM differed significantly in their sexual behavior, the former having higher numbers of partners, more frequent sexual contacts and less frequency of condom use. One third of the men (gay, 3%; bisexual, 34%, heterosexual, 51%; other, 49%) reported having had sex with men, women and transvestites in the two months prior to the interview. This population requires further study and, potentially, HIV prevention strategies tailored to such diversity of partnerships. Our results highlight the potential effectiveness of using RDS to reach non-gay identified MSM. They also present lessons learned in the implementation of RDS to recruit MSM concerning both the importance and limitations of formative work, the need to tailor incentives to circumstances of the less affluent potential participants, the need to prevent masking, and the challenge of assessing network size. PMID:22102896

  16. An early work [1910-1913] in Biological Psychology by pioneer psychiatrist, criminologist and philosopher José Ingenieros, M.D. (1877-1925) of Buenos Aires.

    PubMed

    Triarhou, Lazaros C; del Cerro, Manuel

    2006-04-01

    One of the earliest recorded works in Biological Psychology was published in 1910 by Argentine psychiatrist José Ingenieros (1877-1925), Professor of Experimental Psychology at the Faculty of Philosophy and Letters of the University of Buenos Aires. Ingenieros, a multifaceted personality and prolific author and educator famous for his lapidary aphorisms, has been considered a 'luminary' for generations. Trained as a physician, he was the first scientist to establish a comprehensive psychological system in Latin America. His long list of publications includes more than 300 titles generally divided in two periods: studies in mental pathology and criminology (1897-1908) and studies in philosophy, psychology and sociology (1908-1925). His works were never made particularly available to English-speaking audiences, despite the fact that certain of his books are still best-sellers in the Spanish-speaking world. We present an overview of Ingenieros' life and work, and a detailed account of his profoundly interesting work Principios de Psicología Biológica, in which he analyzes the development, evolution and social context of mental functions. We also provide an English translation of the Introduction contributed by Nobel laureate Wilhelm Ostwald (1853-1932) to the 1922 German edition of the work, pertinent to the energetic principles Ingenieros used and the study of Psychology as a natural science. It is a hope, 80 years after Ingenieros' parting, to bibliographically resurrect this champion of reason, who, until now, has not been given his due placement in the international psychological and biomedical literature.

  17. Social and environmental health determinants and their relationship with parasitic diseases in asymptomatic children from a shantytown in Buenos Aires, Argentina.

    PubMed

    Garbossa, Graciela; Pía Buyayisqui, María; Geffner, Laura; López Arias, Ludmila; de la Fournière, Sofía; Haedo, Ana S; Marconi, Adela E; Frid, Juan C; Nesse, Alcira B; Bordoni, Noemí

    2013-04-01

    Health inequities are a common problem for all countries and are the result of not only adverse social conditions but also poor public policies. Today chronic diseases represent the most relevant threats and are a current challenge. Parasitic infections, a leading cause of child morbidity affecting low-income populations, can be transmitted because of an unhealthy environment. Notwithstanding, scarce data have been published on the epidemiological profile of intestinal parasitoses in asymptomatic children living in shantytowns. Vulnerable populations settled in slums are growing in Argentina, particularly in Buenos Aires city. Consequently, this work intended to screen healthy carriers of enteric parasites and determine the epidemiologic profile in asymptomatic children residing in one of those communities, to explore risk factors associated with the transmission of parasites, and to initiate a basic health education campaign to promote healthy behavior in the community. Fecal samples (n = 138) were analyzed by conventional parasitological methods and a survey gathered data on symptoms, family composition, and environmental and hygiene-related variables. High prevalence of feco-orally-transmitted parasitoses (83·3%) and polyparasitism were remarkable findings. The main environmental health determinants were those related to excreta disposal and water provision. Health promotion actions were performed through the diffusion of a set of posters with iconic images and brief messages for health education. Results suggest the need for an environmental sanitation policy to complement health promotion actions. It is essential to spread the results of investigations that address inequities and social determinants of health in order to integrate data with local political processes and alert on acceptable actions for developing appropriate interventions.

  18. Subduction of the South Chile active spreading ridge: A 17 Ma to 3 Ma magmatic record in central Patagonia (western edge of Meseta del Lago Buenos Aires, Argentina)

    NASA Astrophysics Data System (ADS)

    Boutonnet, E.; Arnaud, N.; Guivel, C.; Lagabrielle, Y.; Scalabrino, B.; Espinoza, F.

    2010-01-01

    The Chile Triple Junction is a natural laboratory to study the interactions between magmatism and tectonics during the subduction of an active spreading ridge beneath a continent. The MLBA plateau (Meseta del Lago Buenos Aires) is one of the Neogene alkali basaltic plateaus located in the back-arc region of the Andean Cordillera at the latitude of the current Chile Triple Junction. The genesis of MLBA can be related with successive opening of slabs windows beneath Patagonia: within the subducting Nazca Plate itself and between the Nazca and Antarctic plates. Detailed 40Ar/ 39Ar dating and geochemical analysis of bimodal magmatism from the western flank of the MLBA show major changes in the back-arc magmatism which occurred between 14.5 Ma and 12.5 Ma with the transition from calc-alkaline lavas (Cerro Plomo) to alkaline lavas (MLBA) in relation with slab window opening. In a second step, at 4-3 Ma, alkaline felsic intrusions were emplaced in the western flank of the MLBA coevally with the MLBA basalts with which they are genetically related. These late OIB-like alkaline to transitional basalts were generated by partial melting of the subslab asthenosphere of the subducting Nazca plate during the opening of the South Chile spreading ridge-related slab window. These basalts differentiated with small amounts of assimilation in shallow magma chambers emplaced along transtensional to extensional zones. The close association of bimodal magmatism with extensional tectonic features in the western MLBA is a strong support to the model of Patagonian collapse event proposed to have taken place between 5 and 3 Ma as a consequence of the presence of the asthenospheric window (SCR-1 segment of South Chile Ridge) below the MLBA area.

  19. Characterization of Shiga Toxin-Producing Escherichia coli Isolated from Ground Beef Collected in Different Socioeconomic Strata Markets in Buenos Aires, Argentina

    PubMed Central

    Llorente, Patricia; Barnech, Laura; Irino, Kinue; Rumi, María Valeria

    2014-01-01

    Consumption of raw/undercooked ground beef is the most common route of transmission of Shiga toxin-producing E. coli (STEC). The aim of the study was to determine the STEC contamination level of the ground beef samples collected in 36 markets of different socioeconomic strata in Buenos Aires, Argentina, and the characterization of the isolated strains. Ninety-one out of 252 (36.1%) samples were stx+. Fifty-seven STEC strains were recovered. Eleven STEC strains belonged to O157 serogroup, and 46 to non-O157 serogroups. Virulence markers of the 57 STEC were stx1, 5.3% (3/57); stx2, 86.0% (49/57); stx1/stx2, 8.8% (5/57); ehxA, 61.4% (35/57); eae, 26.3% (15/57); saa, 24.6% (14/57). Shiga toxin subtypes were stx2, 31.5% (17/54); stx2c-vhb, 24.1% (13/54); stx2c-vha, 20.4% (11/54); stx2/stx2c-vha, 14.8% (8/54); stx2/stx2c-vhb, 5.6% (3/54); stx2c-vha/vhb, 3.7% (2/54). Serotypes O178:H19 and O157:H7 were prevalent. Contamination rate of STEC in all strata was high, and the highest O157 contamination was observed at low strata at several sampling rounds. Persistence of STEC was not detected. Sixteen strains (28.1%) were resistant to ampicillin, streptomycin, amikacin, or tetracycline. The STEC contamination level of ground beef could vary according to the sociocultural characteristics of the population. PMID:25006586

  20. [Cultural adaptation and Argentine validation of the Northwick Park Neck Pain Questionnaire in the hospitals of the Autonomous City of Buenos Aires].

    PubMed

    Aguirre, Mariana V; Rodríguez, Matias G; Clarett, Martín; Iribarne, Juan I; Martínez, Marianela; Battistotti, Romina; López de Arcaute, Ana S; Adarves, Romina; Orsini, Esteban

    2013-01-01

    Objetivo: realizar la adaptación cultural y validación del Cuestionario Northwick Park (NPQ) en Argentina, determinando sus propiedades psicométricas, en pacientes con dolor cervical de origen mecánico derivados al Servicio de Kinesiología del Hospital D. F. Santojanni. Materiales y Métodos: Se solicitó la autorización del autor original del NPQ, luego se realizó la adaptación lingüística y prueba piloto. Las propiedades psicométricas incluyeron: confiabilidad test-retest (coeficiente de correlación intraclase, CCI), validez (coeficiente de correlación de Pearson NPQ-Escala Análoga Visual, EVA), consistencia interna (alfa de Cronbach) y sensibilidad al cambio (prueba T para pruebas pareadas NPQ-EVA). Se incluyeron consecutivamente 60 pacientes de septiembre de 2007 a febrero de 2009 con dolor cervical mecánico. Se midieron las variables porcentaje de discapacidad (NPQ) y dolor (EVA) al día del ingreso, a las 24 horas y al alta. Resultados: Veintiséis pacientes completaron el estudio, 4 fueron eliminados y 30 no completaron la 3° medición. Se obtuvo una buena confiabilidad test-retest (ICC 0.8979) y una alta consistencia interna (alfa de Cronbach 0.86). La validez mostró una buena correlación (r= 0,678). La sensibilidad al cambio fue buena (r=0.661). Conclusión: El NPQ es un instrumento válido, confiable y sensible para evaluar la discapacidad asociada al dolor cervical de origen mecánico en pacientes atendidos en el ámbito hospitalario de la Ciudad Autónoma de Buenos Aires.

  1. Social and environmental health determinants and their relationship with parasitic diseases in asymptomatic children from a shantytown in Buenos Aires, Argentina.

    PubMed

    Garbossa, Graciela; Pía Buyayisqui, María; Geffner, Laura; López Arias, Ludmila; de la Fournière, Sofía; Haedo, Ana S; Marconi, Adela E; Frid, Juan C; Nesse, Alcira B; Bordoni, Noemí

    2013-04-01

    Health inequities are a common problem for all countries and are the result of not only adverse social conditions but also poor public policies. Today chronic diseases represent the most relevant threats and are a current challenge. Parasitic infections, a leading cause of child morbidity affecting low-income populations, can be transmitted because of an unhealthy environment. Notwithstanding, scarce data have been published on the epidemiological profile of intestinal parasitoses in asymptomatic children living in shantytowns. Vulnerable populations settled in slums are growing in Argentina, particularly in Buenos Aires city. Consequently, this work intended to screen healthy carriers of enteric parasites and determine the epidemiologic profile in asymptomatic children residing in one of those communities, to explore risk factors associated with the transmission of parasites, and to initiate a basic health education campaign to promote healthy behavior in the community. Fecal samples (n = 138) were analyzed by conventional parasitological methods and a survey gathered data on symptoms, family composition, and environmental and hygiene-related variables. High prevalence of feco-orally-transmitted parasitoses (83·3%) and polyparasitism were remarkable findings. The main environmental health determinants were those related to excreta disposal and water provision. Health promotion actions were performed through the diffusion of a set of posters with iconic images and brief messages for health education. Results suggest the need for an environmental sanitation policy to complement health promotion actions. It is essential to spread the results of investigations that address inequities and social determinants of health in order to integrate data with local political processes and alert on acceptable actions for developing appropriate interventions. PMID:23683369

  2. The occurrence of Shiga toxin-producing Escherichia coli in bathing water of the Sierra de la Ventana region, Buenos Aires Province, Argentina.

    PubMed

    Marucci, Patricia L; Olivera, Nelda L; Brugnoni, Lorena I; Sica, Maria G; Cubitto, Maria Amelia

    2011-04-01

    The region of Sierra de la Ventana is located in the southwest of Buenos Aires Province, Argentina. Traditionally, this area has been devoted to livestock and agriculture, but tourism has had a significant development in recent years. In the region, there are many rivers and streams that are used for swimming and bathing. A survey of the occurrence of Shiga toxin-producing Escherichia coli (STEC) in these waters was conducted, and the microbiological quality of rivers and streams was investigated. No E. coli O157 was recovered by immunomagnetic separation. Nevertheless, the Shiga toxin gene, exclusively stx2 genotype, was detected in four non-O157 E. coli strains. Two STEC strains carried eae factor, but none of them harbored the EHEC-hlyA gene. Three of the STEC isolates belonged to samples obtained in the warm months, and one to the winter sampling. In the sample sites where STEC strains were isolated the counts of E. coli/100 ml exceeded or were close to the limit recommended by the United States Environmental Protection Agency for bathing water. The relationship observed between the rainy season and E. coli counts suggests that among the main causes for the hygienic indicator increase is the runoff of manure deposited on soils that may also induce the entrance of pathogens into the aquatic environment. This research, the first reporting STEC isolation from recreational waters in this area, revealed that streams and rivers from a beef-producing area of Argentina are a reservoir of STEC strains.

  3. HIV and Other Sexually Transmitted Infections among Men Who Have Sex with Men Recruited by RDS in Buenos Aires, Argentina: High HIV and HPV Infection

    PubMed Central

    Pando, María A.; Balán, Iván C.; Marone, Rubén; Dolezal, Curtis; Leu, Cheng-Shiun; Squiquera, Luis; Barreda, Victoria; Rodriguez Fermepín, Marcelo; Gallo Vaulet, Lucia; Rey, Jorge; Picconi, María; Carballo-Diéguez, Alex; Avila, María M.

    2012-01-01

    Background The aim of this study was to estimate the prevalence of HIV and other STIs, among MSM from Buenos Aires (2007–2009). Methods Responding Driven Sampling was used for recruitment of MSM. Participants completed a structured web-based survey and provided biological samples. Results A total of 496 MSM were studied for HIV, HBV, HCV, and T pallidum infections. Chlamydia and HPV diagnoses were only performed in 98 and 109 participants, respectively. Prevalence of HIV was 17.3%, HBV 22.9%, HCV 7.5%, T pallidum 20.5%, HPV 83.5%, and C trachomatis 1.7%. In the year prior to the evaluation, 71% of the participants had had sex with men and/or trans and women (MMW) while 29% had not had sex with women (MM). Comparing MM to MMW, prevalence of HIV (30.7% vs. 11.9%, p<0.001), HBV (36.4% vs. 17.8%, p<0.001), T pallidum (32.1% vs. 15.7%, p<0.001), and HPV (88.3% vs. 70.4%, p = 0.039) were significantly higher among MM, whereas no significant differences were found for HCV and C trachomatis. The MM group had also significantly higher HIV incidence (5.60 vs. 4.28 per 100 persons-year, p = 0.032). HPV genotypes 16, 6, and 11 were the most frequently found; 40.7% of the MSM had more than one genotype and one high risk genotype was detected in 43.6% of participants. Conclusions Both MM and MMW are at high risk of infection for HIV and other STIs. Rates of HIV, HBV, T pallidum and HPV infections are higher in the MM group. PMID:22768137

  4. Incorporating Community Knowledge to Lahar Hazard Maps: Canton Buenos Aires Case Study, at Santa Ana (Ilamatepec) Volcano

    NASA Astrophysics Data System (ADS)

    Bajo, J. V.; Martinez-Hackert, B.; Polio, C.; Gutierrez, E.

    2015-12-01

    Santa Ana (Ilamatepec) Volcano is an active composite volcano located in the Apaneca Volcanic Field located in western part of El Salvador, Central America. The volcano is surrounded by rural communities in its proximal areas and the second (Santa Ana, 13 km) and fourth (Sonsosante, 15 km) largest cities of the country. On October 1st, 2005, the volcano erupted after months of increased activity. Following the eruption, volcanic mitigation projects were conducted in the region, but the communities had little or no input on them. This project consisted in the creation of lahar volcanic hazard map for the Canton Buanos Aires on the northern part of the volcano by incorporating the community's knowledge from prior events to model parameters and results. The work with the community consisted in several meetings where the community members recounted past events. They were asked to map the outcomes of those events using either a topographic map of the area, a Google Earth image, or a blank paper poster size. These maps have been used to identify hazard and vulnerable areas, and for model validation. These maps were presented to the communities and they accepted their results and the maps.

  5. Low frequency of male circumcision and unwillingness to be circumcised among MSM in Buenos Aires, Argentina: association with sexually transmitted infections

    PubMed Central

    Pando, María A; Balan, Ivan C; Dolezal, Curtis; Marone, Ruben; Barreda, Victoria; Carballo-Dieguez, Alex; Avila, María M

    2013-01-01

    Objective The aims of this study were to investigate the frequency of male circumcision among men who have sex with men (MSM) in Buenos Aires, Argentina; the association between circumcision and sexually transmitted infections (STIs); and, among those uncircumcised, the willingness to be circumcised. Methods A cross-sectional study was conducted among 500 MSM recruited through the respondent-driven sampling (RDS) technique. Participants underwent a consent process, responded to a Web-based survey that included questions on demographic information, sexual behaviour, and circumcision and provided biological samples. HIV, hepatitis B virus (HBV), hepatitis C virus (HCV), Treponema pallidum, and human papiloma virus (HPV) diagnoses were performed using standard methodologies. For all analyses, data were weighted based on participants’ network size. Results Only 64 (13%) of the 500 MSM in our study reported being circumcised. Among uncircumcised men (n=418), 302 (70.4%) said that they would not be willing to get circumcised even if the procedure could reduce the risk of HIV infection. When considering all participants, circumcision status was not significantly associated with HIV, HBV, HCV, T. pallidum or HPV infections. However, when we restricted the sample to men who do not practice receptive anal intercourse (RAI) and compared circumcised to uncircumcised men, the former (N=33) had no cases of HIV infection, while 34 of 231 (14.8%) uncircumcised men were HIV positive (p=0.020). Regarding HPV, uncircumcised men had a significantly larger number of different HPV types compared with circumcised men (mean 1.83 vs. 1.09, p<0.001) and a higher frequency of high-risk-HPV genotypes (47.6% vs. 12.5%, p=0.012). Conclusions Consistent with international evidence, male circumcision appears to have a partial protective effect among MSM. The efficacy of circumcision in reducing risk of HIV infection among MSM appears to be correlated with sexual practices. Given the lack of

  6. Magnitude and distribution of canine fecal contamination and helminth eggs in two areas of different urban structure, Greater Buenos Aires, Argentina.

    PubMed

    Rubel, D; Wisnivesky, C

    2005-11-01

    The canine fecal contamination and the potential transmission of parasites to human and canine populations represent a public health problem of cosmopolitan importance. The objectives of this work were to evaluate the magnitude of fecal and parasite contamination in two suburban areas of different characteristics, and to investigate their distribution in various urban microenvironments such as yards, green spaces and sidewalks. The areas were referred to as middle-income neighbourhood (MIN) and low-income neighbourhood (LIN). To evaluate fecal contamination and its distribution, feces were counted and areas surveyed were calculated. The parasite contamination was evaluated from the prevalence of helminthosis found in a randomly selected fecal sample of the canine population in each of the study areas. The respective median fecal densities in MIN and LIN were 0.11 and 0.12 feces/m2 for green spaces, 0.10 and 0.19 feces/m2 for sidewalks (Mann-Whitney test, p<0.05), and 0.04 and 0.07 feces/m2 for households. In all the surveyed environments, dogs were free-ranging animals and preferred to defecate on grass surfaces rather than on bare soil, tile or sand. In MIN and LIN the respective prevalences were 40% and 70% for helminths in general (Chi square=15.17, p<0.01), 14 and 53% for Ancylostoma (Chi square=23.99, p<0.01), 9 and 17% for Toxocara (p>0.05), and 26 and 38% for Trichuris (p>0.05). Sidewalks were the most contaminated environments in LIN. The level of infected feces in sidewalks and yards was higher in LIN than in MIN (Mann-Whitney test, p<0.05). This study includes a discussion of the influence of variables such as canine population density, sidewalk structure and amount of available green spaces on the distribution of fecal contamination, and results obtained are compared with those previously recorded for Buenos Aires City. An increasing gradient of contamination by canine feces and parasites was observed as socioeconomic status decreased, the canine

  7. [The case of the 'lowlife': perceptions of danger and the prevention of disreputable behaviors in theRevista de Criminología, Psiquiatría, Medicina Legal y Ciencias Afinesin Buenos Aires, 1914-1923].

    PubMed

    Dovio, Mariana

    2013-11-30

    We analyze the construction of the notion of the 'lowlife' in the city of Buenos Aires in the Revista de Criminología, Psiquiatría y Medicina Legal, from 1914-1923. We shall analyze the notion of 'lowlife', meaning behaviors situated in a border zone between crime and madness, from the point of view of prevention and perception of danger. The first way of analyzing the 'lowlife' examines institutional projects that were related to eugenics and that advocated preventive detention of dangerous people, 'alcoholists' and vagrants. The second involves identifying in moral and physical terms the kind of people who were targeted in the creation of intervention projects for those considered to be 'lowlifes', who could potentially damage the fabric of society in some way.

  8. [The case of the 'lowlife': perceptions of danger and the prevention of disreputable behaviors in theRevista de Criminología, Psiquiatría, Medicina Legal y Ciencias Afinesin Buenos Aires, 1914-1923].

    PubMed

    Dovio, Mariana

    2013-11-30

    We analyze the construction of the notion of the 'lowlife' in the city of Buenos Aires in the Revista de Criminología, Psiquiatría y Medicina Legal, from 1914-1923. We shall analyze the notion of 'lowlife', meaning behaviors situated in a border zone between crime and madness, from the point of view of prevention and perception of danger. The first way of analyzing the 'lowlife' examines institutional projects that were related to eugenics and that advocated preventive detention of dangerous people, 'alcoholists' and vagrants. The second involves identifying in moral and physical terms the kind of people who were targeted in the creation of intervention projects for those considered to be 'lowlifes', who could potentially damage the fabric of society in some way. PMID:24346199

  9. Curricular Guidelines in Biochemistry.

    ERIC Educational Resources Information Center

    Adams, A. Birk; And Others

    1981-01-01

    Curricular guidelines for biochemistry are presented, developed by the Section on Biochemistry and Nutrition and the Section on Oral Diagnosis and Oral Medicine of the American Association of Dental Schools for use by individual educational institutions as curriculum development aids. (MLW)

  10. Changes in time-use and drug use by young adults in poor neighbourhoods of Greater Buenos Aires, Argentina, after the political transitions of 2001-2002: Results of a survey

    PubMed Central

    2011-01-01

    Background In some countries, "Big Events" like crises and transitions have been followed by large increases in drug use, drug injection and HIV/AIDS. Argentina experienced an economic crisis and political transition in 2001/2002 that affected how people use their time. This paper studies how time use changes between years 2001 and 2004, subsequent to these events, were associated with drug consumption in poor neighbourhoods of Greater Buenos Aires. Methods In 2003-2004, 68 current injecting drug users (IDUs) and 235 young non-IDUs, aged 21-35, who lived in impoverished drug-impacted neighbourhoods in Greater Buenos Aires, were asked about time use then and in 2001. Data on weekly hours spent working or looking for work, doing housework/childcare, consuming drugs, being with friends, and hanging out in the neighbourhood, were studied in relation to time spent using drugs. Field observations and focus groups were also conducted. Results After 2001, among both IDUs and non-IDUs, mean weekly time spent working declined significantly (especially among IDUs); time spent looking for work increased, and time spent with friends and hanging out in the neighbourhood decreased. We found no increase in injecting or non-injecting drug consumption after 2001. Subjects most affected by the way the crises led to decreased work time and/or to increased time looking for work--and by the associated increase in time spent in one's neighbourhood--were most likely to increase their time using drugs. Conclusions Time use methods are useful to study changes in drug use and their relationships to every day life activities. In these previously-drug-impacted neighbourhoods, the Argentinean crisis did not lead to an increase in drug use, which somewhat contradicts our initial expectations. Nevertheless, those for whom the crises led to decreased work time, increased time looking for work, and increased time spent in indoor or outdoor neighbourhood environments, were likely to spend more time

  11. Reproduction, physiology and biochemistry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This chapter summarizes fundamental knowledge and recent discoveries about the reproduction, physiology and biochemistry of plant-parasitic nematodes. Various types of reproduction are reviewed, including sexual reproduction and mitotic and meiotic parthenogenesis. Although much is known about the p...

  12. Aceptabilidad del diagnóstico rápido casero para HIV entre hombres gay y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires.

    PubMed

    Balán, Iván C; Carballo-Diéguez, Alex; Marone, Rubén O; Pando, María A; Barreda, Victoria; Avila, María M

    2011-03-01

    El uso del diagnóstico rápido para HIV en Argentina, así como otros países de Latinoamérica, ha sido limitado hasta el momento. Este trabajo reporta los resultados provenientes de un estudio cualitativo realizado entre hombres gays y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires, Argentina. El objetivo principal del mismo fue conocer las ventajas y desventajas que los hombres G&HSH perciben en relación al diagnóstico rápido casero para HIV. Se realizaron ocho grupos focales con 73 participantes en los cuales se discutió acerca de las ventajas y desventajas del uso de los diagnósticos rápidos. Las respuestas fueron codificadas utilizando un programa para análisis de datos cualitativos (NVivo) y analizadas temáticamente. Los participantes describieron numerosas ventajas sobre el uso del diagnóstico rápido casero, aunque algunos reportaron importantes preocupaciones dentro de las cuales se destaca la posibilidad de impulsos suicidas si alguien recibe un resultado positivo estando solo. En términos generales se observó una gran aceptabilidad para el uso del diagnóstico rápido si el mismo es realizado por personal de salud en lugares acondicionados para este fin.

  13. Aceptabilidad del diagnóstico rápido casero para HIV entre hombres gay y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires

    PubMed Central

    Balán, Iván C.; Carballo-Diéguez, Alex; Marone, Rubén O.; Pando, María A.; Barreda, Victoria; Ávila, María M.

    2011-01-01

    Resumen El uso del diagnóstico rápido para HIV en Argentina, así como otros países de Latinoamérica, ha sido limitado hasta el momento. Este trabajo reporta los resultados provenientes de un estudio cualitativo realizado entre hombres gays y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires, Argentina. El objetivo principal del mismo fue conocer las ventajas y desventajas que los hombres G&HSH perciben en relación al diagnóstico rápido casero para HIV. Se realizaron ocho grupos focales con 73 participantes en los cuales se discutió acerca de las ventajas y desventajas del uso de los diagnósticos rápidos. Las respuestas fueron codificadas utilizando un programa para análisis de datos cualitativos (NVivo) y analizadas temáticamente. Los participantes describieron numerosas ventajas sobre el uso del diagnóstico rápido casero, aunque algunos reportaron importantes preocupaciones dentro de las cuales se destaca la posibilidad de impulsos suicidas si alguien recibe un resultado positivo estando solo. En términos generales se observó una gran aceptabilidad para el uso del diagnóstico rápido si el mismo es realizado por personal de salud en lugares acondicionados para este fin. PMID:25284951

  14. Aceptabilidad del diagnóstico rápido casero para HIV entre hombres gay y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires.

    PubMed

    Balán, Iván C; Carballo-Diéguez, Alex; Marone, Rubén O; Pando, María A; Barreda, Victoria; Avila, María M

    2011-03-01

    El uso del diagnóstico rápido para HIV en Argentina, así como otros países de Latinoamérica, ha sido limitado hasta el momento. Este trabajo reporta los resultados provenientes de un estudio cualitativo realizado entre hombres gays y otros hombres que tienen sexo con hombres (G&HSH) de la Ciudad de Buenos Aires, Argentina. El objetivo principal del mismo fue conocer las ventajas y desventajas que los hombres G&HSH perciben en relación al diagnóstico rápido casero para HIV. Se realizaron ocho grupos focales con 73 participantes en los cuales se discutió acerca de las ventajas y desventajas del uso de los diagnósticos rápidos. Las respuestas fueron codificadas utilizando un programa para análisis de datos cualitativos (NVivo) y analizadas temáticamente. Los participantes describieron numerosas ventajas sobre el uso del diagnóstico rápido casero, aunque algunos reportaron importantes preocupaciones dentro de las cuales se destaca la posibilidad de impulsos suicidas si alguien recibe un resultado positivo estando solo. En términos generales se observó una gran aceptabilidad para el uso del diagnóstico rápido si el mismo es realizado por personal de salud en lugares acondicionados para este fin. PMID:25284951

  15. Land plant biochemistry.

    PubMed

    Raven, J A

    2000-06-29

    Biochemical studies have complemented ultrastructural and, subsequently molecular genetic evidence consistent with the Charophyceae being the closest extant algal relatives of the embryophytes. Among the genes used in such molecular phylogenetic studies is that rbcL) for the large subunit of ribulose bisphosphate carboxylase-oxygenase (RUBISCO). The RUBISCO of the embryophytes is derived, via the Chlorophyta. from that of the cyanobacteria. This clade of the molecular phylogeny of RUBISCO shows a range of kinetic characteristics, especially of CO2 affinities and of CO2/O2 selectivities. The range of these kinetic values within the bryophytes is no greater than in the rest of the embryophytes; this has implications for the evolution of the embryophytes in the high atmospheric CO2 environment of the late Lower Palaeozoic. The differences in biochemistry between charophycean algae and embryophytes can to some extent be related functionally to the structure and physiology of embryophytes. Examples of components of embryophytes, which are qualitatively or quantitatively different from those of charophytes, are the water repellent/water resistant extracellular lipids, the rigid phenolic polymers functional in water-conducting elements and mechanical support in air, and in UV-B absorption, flavonoid phenolics involved in UV-B absorption and in interactions with other organisms, and the greater emphasis on low Mr organic acids. retained in the plant as free acids or salts, or secreted to the rhizosphere. The roles of these components are discussed in relation to the environmental conditions at the time of evolution of the terrestrial embryophytes. A significant point about embryophytes is the predominance of nitrogen-free extracellular structural material (a trait shared by most algae) and UV-B screening components, by contrast with analogous components in many other organisms. An important question, which has thus far been incompletely addressed, is the extent to which

  16. Land plant biochemistry.

    PubMed Central

    Raven, J A

    2000-01-01

    Biochemical studies have complemented ultrastructural and, subsequently molecular genetic evidence consistent with the Charophyceae being the closest extant algal relatives of the embryophytes. Among the genes used in such molecular phylogenetic studies is that rbcL) for the large subunit of ribulose bisphosphate carboxylase-oxygenase (RUBISCO). The RUBISCO of the embryophytes is derived, via the Chlorophyta. from that of the cyanobacteria. This clade of the molecular phylogeny of RUBISCO shows a range of kinetic characteristics, especially of CO2 affinities and of CO2/O2 selectivities. The range of these kinetic values within the bryophytes is no greater than in the rest of the embryophytes; this has implications for the evolution of the embryophytes in the high atmospheric CO2 environment of the late Lower Palaeozoic. The differences in biochemistry between charophycean algae and embryophytes can to some extent be related functionally to the structure and physiology of embryophytes. Examples of components of embryophytes, which are qualitatively or quantitatively different from those of charophytes, are the water repellent/water resistant extracellular lipids, the rigid phenolic polymers functional in water-conducting elements and mechanical support in air, and in UV-B absorption, flavonoid phenolics involved in UV-B absorption and in interactions with other organisms, and the greater emphasis on low Mr organic acids. retained in the plant as free acids or salts, or secreted to the rhizosphere. The roles of these components are discussed in relation to the environmental conditions at the time of evolution of the terrestrial embryophytes. A significant point about embryophytes is the predominance of nitrogen-free extracellular structural material (a trait shared by most algae) and UV-B screening components, by contrast with analogous components in many other organisms. An important question, which has thus far been incompletely addressed, is the extent to which

  17. Land plant biochemistry.

    PubMed

    Raven, J A

    2000-06-29

    Biochemical studies have complemented ultrastructural and, subsequently molecular genetic evidence consistent with the Charophyceae being the closest extant algal relatives of the embryophytes. Among the genes used in such molecular phylogenetic studies is that rbcL) for the large subunit of ribulose bisphosphate carboxylase-oxygenase (RUBISCO). The RUBISCO of the embryophytes is derived, via the Chlorophyta. from that of the cyanobacteria. This clade of the molecular phylogeny of RUBISCO shows a range of kinetic characteristics, especially of CO2 affinities and of CO2/O2 selectivities. The range of these kinetic values within the bryophytes is no greater than in the rest of the embryophytes; this has implications for the evolution of the embryophytes in the high atmospheric CO2 environment of the late Lower Palaeozoic. The differences in biochemistry between charophycean algae and embryophytes can to some extent be related functionally to the structure and physiology of embryophytes. Examples of components of embryophytes, which are qualitatively or quantitatively different from those of charophytes, are the water repellent/water resistant extracellular lipids, the rigid phenolic polymers functional in water-conducting elements and mechanical support in air, and in UV-B absorption, flavonoid phenolics involved in UV-B absorption and in interactions with other organisms, and the greater emphasis on low Mr organic acids. retained in the plant as free acids or salts, or secreted to the rhizosphere. The roles of these components are discussed in relation to the environmental conditions at the time of evolution of the terrestrial embryophytes. A significant point about embryophytes is the predominance of nitrogen-free extracellular structural material (a trait shared by most algae) and UV-B screening components, by contrast with analogous components in many other organisms. An important question, which has thus far been incompletely addressed, is the extent to which

  18. Potential alternate life biochemistries

    NASA Astrophysics Data System (ADS)

    Konesky, Gregory

    2013-09-01

    While life on Earth continues to be discovered in unlikely environments, the underlying biochemistry is all very similar, based on the element carbon, and requiring liquid water. We consider alternate biochemistries based on elements other than carbon, including other group IVA elements, such as silicon and germanium, and solvents other than water. Terminal electron acceptors other than oxygen are also discussed. A fundamental issue is raised related to the detection of, and even the definition of life, whether it is carbon or non-carbon based. An extreme example of this issue would be in consideration of speculative life based on electrically charged dusty plasmas, which may have no physical body.

  19. Association of monthly frequencies of diverse diseases in the calls to the public emergency service of the city of Buenos Aires during 1999-2004 with meteorological variables and seasons.

    PubMed

    Alexander, P

    2013-01-01

    This work aims to study associations between monthly averages of meteorological variables and monthly frequencies of diverse diseases in the calls to the public ambulance emergency service of the city of Buenos Aires during the years 1999-2004. Throughout this time period no changes were made in the classification codes of the illnesses. Heart disease, arrhythmia, heart failure, cardiopulmonary arrest, angina pectoris, psychiatric diseases, stroke, transient ischemic attack, syncope and the total number of calls were analyzed against 11 weather variables and the four seasons. All illnesses exhibited some seasonal behavior, except cardiorespiratory arrest and angina pectoris. The largest frequencies of illnesses that exhibited some association with the meteorological variables used to occur in winter, except the psychiatric cases. Heart failure, stroke, psychiatric diseases and the total number of calls showed significant correlations with the 11 meteorological variables considered, and the largest indices (absolute values above 0.6) were found for the former two pathologies. On the other side, cardiorespiratory arrest and angina pectoris revealed no significant correlations and nearly null indices. Variables associated with temperature were the meteorological proxies with the largest correlations against diseases. Pressure and humidity mostly exhibited positive correlations, which is the opposite of variables related to temperature. Contrary to all other diseases, psychiatric pathologies showed a clear predominance of positive correlations. Finally, the association degree of the medical dataset with recurrent patterns was further evaluated through Fourier analysis, to assess the presence of statistically significant behavior. In the Northern Hemisphere high morbidity and mortality rates in December are usually assigned to diverse factors in relation to the holidays, but such an effect is not observed in the present analysis. There seems to be no clearly preferred

  20. Subduction of the South-Chile active spreading ridge: a 17 Ma to 3 Ma magmatic record in central Patagonia (western edge of Meseta del Lago Buenos Aires, Argentina)

    NASA Astrophysics Data System (ADS)

    Boutonnet, Emmanuelle; Arnaud, Nicolas; Guivel, Christèle; Lagabrielle, Yves; Scalabrino, Bruno; Espinoza, Felipe

    2010-05-01

    The Chile Triple Junction is a natural laboratory to study the interactions between magmatism and tectonics during the subduction of an active spreading ridge beneath a continent. The MLBA plateau (Meseta del Lago Buenos Aires) is one of the Neogene alkali basaltic plateaus located in the back-arc region of the Andean Cordillera at the latitude of the current Chile Triple Junction. The genesis of MLBA can be related with successive opening of slabs windows beneath Patagonia: within the subducting Nazca Plate itself and between the Nazca and Antarctic plates. Detailed 40Ar/39Ar dating and geochemical analysis of bimodal magmatism from the western flank of the MLBA show major changes in the back-arc magmatism which occurred between 14.5 Ma and 12.5 Ma with the transition from calc-alkaline lavas (Cerro Plomo) to alkaline lavas (MLBA) in relation with slab window opening. In a second step, at 4- 3 Ma, alkaline felsic intrusions were emplaced in the western flank of the MLBA coevally with the MLBA basalts with which they are genetically related. These late OIB-like alkaline to transitional basalts were generated by partial melting of the subslab asthenosphere of the subducting Nazca plate during the opening of the South Chile spreading ridge-related slab window. These basalts differentiated with small amounts of assimilation in shallow magma chambers emplaced along transtensional to extensional zones. The close association of bimodal magmatism with extensional tectonic features in the western MLBA is a strong support to the model of Patagonian collapse event proposed to have taken place between 5 and 3 Ma as a consequence of the presence of the asthenospheric window (SCR-1 segment of South Chile Ridge) below the MLBA area.

  1. Association of monthly frequencies of diverse diseases in the calls to the public emergency service of the city of Buenos Aires during 1999-2004 with meteorological variables and seasons

    NASA Astrophysics Data System (ADS)

    Alexander, P.

    2013-01-01

    This work aims to study associations between monthly averages of meteorological variables and monthly frequencies of diverse diseases in the calls to the public ambulance emergency service of the city of Buenos Aires during the years 1999-2004. Throughout this time period no changes were made in the classification codes of the illnesses. Heart disease, arrhythmia, heart failure, cardiopulmonary arrest, angina pectoris, psychiatric diseases, stroke, transient ischemic attack, syncope and the total number of calls were analyzed against 11 weather variables and the four seasons. All illnesses exhibited some seasonal behavior, except cardiorespiratory arrest and angina pectoris. The largest frequencies of illnesses that exhibited some association with the meteorological variables used to occur in winter, except the psychiatric cases. Heart failure, stroke, psychiatric diseases and the total number of calls showed significant correlations with the 11 meteorological variables considered, and the largest indices (absolute values above 0.6) were found for the former two pathologies. On the other side, cardiorespiratory arrest and angina pectoris revealed no significant correlations and nearly null indices. Variables associated with temperature were the meteorological proxies with the largest correlations against diseases. Pressure and humidity mostly exhibited positive correlations, which is the opposite of variables related to temperature. Contrary to all other diseases, psychiatric pathologies showed a clear predominance of positive correlations. Finally, the association degree of the medical dataset with recurrent patterns was further evaluated through Fourier analysis, to assess the presence of statistically significant behavior. In the Northern Hemisphere high morbidity and mortality rates in December are usually assigned to diverse factors in relation to the holidays, but such an effect is not observed in the present analysis. There seems to be no clearly preferred

  2. Association of monthly frequencies of diverse diseases in the calls to the public emergency service of the city of Buenos Aires during 1999-2004 with meteorological variables and seasons.

    PubMed

    Alexander, P

    2013-01-01

    This work aims to study associations between monthly averages of meteorological variables and monthly frequencies of diverse diseases in the calls to the public ambulance emergency service of the city of Buenos Aires during the years 1999-2004. Throughout this time period no changes were made in the classification codes of the illnesses. Heart disease, arrhythmia, heart failure, cardiopulmonary arrest, angina pectoris, psychiatric diseases, stroke, transient ischemic attack, syncope and the total number of calls were analyzed against 11 weather variables and the four seasons. All illnesses exhibited some seasonal behavior, except cardiorespiratory arrest and angina pectoris. The largest frequencies of illnesses that exhibited some association with the meteorological variables used to occur in winter, except the psychiatric cases. Heart failure, stroke, psychiatric diseases and the total number of calls showed significant correlations with the 11 meteorological variables considered, and the largest indices (absolute values above 0.6) were found for the former two pathologies. On the other side, cardiorespiratory arrest and angina pectoris revealed no significant correlations and nearly null indices. Variables associated with temperature were the meteorological proxies with the largest correlations against diseases. Pressure and humidity mostly exhibited positive correlations, which is the opposite of variables related to temperature. Contrary to all other diseases, psychiatric pathologies showed a clear predominance of positive correlations. Finally, the association degree of the medical dataset with recurrent patterns was further evaluated through Fourier analysis, to assess the presence of statistically significant behavior. In the Northern Hemisphere high morbidity and mortality rates in December are usually assigned to diverse factors in relation to the holidays, but such an effect is not observed in the present analysis. There seems to be no clearly preferred

  3. Biochemistry Off the Shelf.

    ERIC Educational Resources Information Center

    Wilson, Jerry L.

    1985-01-01

    Provides sources of nonanimal biochemical materials (which are relatively inexpensive, readily available, and require no special storage) suitable for use in biochemistry experiments. They are presented under these headings: (1) enzymes and other proteins; (2) carbohydrates; (3) lipids; (4) nucleic acids; and (5) metabolism. (JN)

  4. A Freshman Biochemistry Course.

    ERIC Educational Resources Information Center

    De Toma, Francis J.; Campbell, Mary K.

    1982-01-01

    A one-semester biochemistry course was developed as an alternative to traditional freshman chemistry. Lecture topics and laboratory exercises focus on the course's unifying theme of the origin and early stages of the evolution of life on earth. (Author/SK)

  5. Bueno y la autorreparacion ("Bueno" and Conversational Self Repairs).

    ERIC Educational Resources Information Center

    Carranza, Isolda

    The paper discusses the Spanish expression "bueno," which marks the beginning or the end of background and assertion self-repairs in conversation, and often co-occurs with other expressions, such as "viste" and "no?" which mark the other end of the repair. Background repairs provide information the speaker considers necessary for the hearer to…

  6. The biochemistry of memory.

    PubMed

    Stock, Jeffry B; Zhang, Sherry

    2013-09-01

    Almost fifty years ago, Julius Adler initiated a program of research to gain insights into the basic biochemistry of intelligent behavior by studying the molecular mechanisms that underlie the chemotactic responses of Escherichia coli. All living organisms share elements of a common biochemistry for metabolism, growth and heredity - why not intelligence? Neurobiologists have demonstrated that this is the case for nervous systems in animals ranging from worms to man. Motile unicellular organisms such as E. coli exhibit rudimentary behaviors that can be loosely described in terms of cognitive phenomena such as memory and learning. Adler's initiative at least raised the prospect that, because of the numerous experimental advantages provided by E. coli, it would be the first organism whose behavior could be understood at molecular resolution.

  7. Biochemistry and endocrinology results

    SciTech Connect

    Leach, C.S.

    1981-12-01

    Blood (plasma or serum) biochemistry findings show postflight decreases below preflight findings for uric acid triglycerides, and AST. Postflight increases above preflight values were observed in glucose, cholesterol, BUN, calcium phosphate, angiotensin I, aldosterone, insulin, T3, T4, HGH, ACTH and GGTP. It is suggested that special attention should be given to the fluid and electrolyte intake in the astronauts so that homeostatic perturbations are not consequential.

  8. Variation of the Earth's magnetic field strength in South America during the last two millennia: New results from historical buildings of Buenos Aires and re-evaluation of regional data

    NASA Astrophysics Data System (ADS)

    Goguitchaichvili, Avto; Morales, Juan; Schavelzon, Daniel; Vásquez, Carlos; Gogorza, Claudia S. G.; Loponte, Daniel; Rapalini, Augusto

    2015-08-01

    The causes of the systematic decay of the Earth's Magnetic Field strength since eighteen century have been a matter of debate during the last decade. It is also well known that such variations may have completely different expressions under an area characterized with strong magnetic anomalies, such as the South Atlantic Magnetic Anomaly. To fully understand these atypical phenomena, it is crucial to retrieve the past evolution of Earth's magnetic field beyond the observatory records. We report on detailed rock-magnetic and archeointensity investigations from some well-studied historical buildings of Buenos Aires city, located at the heart of the South Atlantic Magnetic Anomaly. Samples consist of bricks, tiles, fireplaces and pottery, which are considered as highly suitable materials for archaeointensity studies. The dating is ascertained by historical documents complemented by archeological constraints. Eighteen out of 26 analyzed samples yield reliable absolute intensity determinations. The site-mean archaeointensity values obtained in this study range from 28.5 to 43.5 μT, with corresponding virtual axial dipole moments (VADMs) ranging from 5.3 to 8.04 × 1022 Am2. Most determinations obtained in the present study are in remarkable agreement with the values predicted by the time varying field model CALS10k.1b (Korte et al., 2011). For the older periods the recently available SHA.DIF.14 model (Pavon-Carrasco et al., 2014) seems to have greater resolution. South American archaeointensity database now includes absolute intensities from 400 to 1930 AD based on 63 selected archaeointensity determinations. The data set reveals several distinct periods of quite large fluctuations of intensity. However, most data are concentrated into a relatively narrow interval from AD 1250 to AD 1450. At the beginning of the record, values between 400 AD and 830 AD match well with ARCH3k.1 model. Some general features may be detected: the time intervals from about AD 400 to 950 and

  9. Thiol biochemistry of prokaryotes

    NASA Technical Reports Server (NTRS)

    Fahey, Robert C.

    1986-01-01

    The present studies have shown that GSH metabolism arose in the purple bacteria and cyanobacteria where it functions to protect against oxygen toxicity. Evidence was obtained indicating that GSH metabolism was incorporated into eucaryotes via the endosymbiosis giving rise to mitochrondria and chloroplasts. Aerobic bacteria lacking GSH utilize other thiols for apparently similar functions, the thiol being coenzyme A in Gram positive bacteria and chi-glutamylcysteine in the halobacteria. The thiol biochemistry of prokaryotes is thus seen to be much more highly diversified than that of eucaryotes and much remains to be learned about this subject.

  10. Update: Biochemistry of Genetic Manipulation.

    ERIC Educational Resources Information Center

    Barker, G. R.

    1983-01-01

    Various topics on the biochemistry of genetic manipulation are discussed. These include genetic transformation and DNA; genetic expression; DNA replication, repair, and mutation; technology of genetic manipulation; and applications of genetic manipulation. Other techniques employed are also considered. (JN)

  11. Biochemistry of Statins.

    PubMed

    Egom, Emmanuel Eroume A; Hafeez, Hafsa

    2016-01-01

    Cardiovascular disease (CVD) is the leading cause of morbidity and mortality worldwide. Elevated blood lipids may be a major risk factor for CVD. Due to consistent and robust association of higher low-density lipoprotein (LDL)-cholesterol levels with CVD across experimental and epidemiologic studies, therapeutic strategies to decrease risk have focused on LDL-cholesterol reduction as the primary goal. Current medication options for lipid-lowering therapy include statins, bile acid sequestrants, a cholesterol-absorption inhibitor, fibrates, nicotinic acid, and omega-3 fatty acids, which all have various mechanisms of action and pharmacokinetic properties. The most widely prescribed lipid-lowering agents are the HMG-CoA reductase inhibitors, or statins. Since their introduction in the 1980s, statins have emerged as the one of the best-selling medication classes to date, with numerous trials demonstrating powerful efficacy in preventing cardiovascular outcomes (Kapur and Musunuru, 2008 [1]). The statins are commonly used in the treatment of hypercholesterolemia and mixed hyperlipidemia. This chapter focuses on the biochemistry of statins including their structures, pharmacokinetics, and mechanism of actions as well as the potential adverse reactions linked to their clinical uses. PMID:26975972

  12. Biochemistry (by Jochanan Stenesh)

    NASA Astrophysics Data System (ADS)

    Glasfeld, Arthur

    1999-06-01

    Plenum: New York, 1998. Hardcover, ISBN 0 306-45732-6. 95. Paperback, ISBN 0 306 45733 4. 55 (set of 3). Solutions manual and transparencies available. According to the promotional materials accompanying this text, its intended audience is students in one-semester undergraduate biochemistry courses. At just over 500 pages, the book is shorter than the norm of well over 1000 pages. The challenge, then, is to present the subject in a coherent and compelling fashion while necessarily omitting a large fraction of the material that one normally finds in more inclusive texts. That kind of editing is obviously going to lead to squawking from some quarters, so I should put my prejudices on the table. I teach a one-semester course in biochemical structure, and I have a long-standing interest in using molecular models to explain biochemical behavior, both in research and in teaching. The editing performed by Professor Stenesh is likely to trouble someone with a structural or mechanistic background. Rather than selectively excluding some topics, Stenesh has created a table of contents that looks like it's from a much longer text. The usual chapters on biochemical structure, catalysis, metabolism and molecular genetics are included here. The ax fell elsewhere, and most obvious to my eye are the omissions of structure and chemical mechanism beyond those few chapters that are dedicated to them. A brief presentation on the structure and function of hemoglobin is given in the chapter on proteins, and the catalytic mechanism of chymotrypsin is briefly presented in the chapter on catalysis. But in chapters on metabolism, the structures of substrates and products are shown while mechanisms of conversion are omitted. For example, in the description of aldolase, we're informed that the enzyme catalyzes a reverse aldol condensation, but the reader isn't shown how the aldol condensation relates to the chemical conversion we see in the figure. (Part of the problem may be that the text

  13. Commentary: Biochemistry Re-Natured

    ERIC Educational Resources Information Center

    White, Harold B.

    2010-01-01

    In his last commentary on "Biochemistry Denatured," this author dealt with his perception that college students today have spent too little of their childhood years playing outside in nature and as a consequence have not learned basic things about the world from personal experience. This "nature-deficit disorder" removes many opportunities for…

  14. Soil Microbiology, Ecology, and Biochemistry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The 4th edition of Soil Microbiology, Ecology, and Biochemistry Edited by Eldor Paul continues in the vein of the 3rd edition by providing an excellent, broad-reaching introduction to soil biology. The new edition improves on the previous by providing extensive supplementary materials, links to outs...

  15. Promising New Directions in Biochemistry

    ERIC Educational Resources Information Center

    Olivera, Baldomero M.

    2003-01-01

    "Biochemistry," by Lubert Stryer, has become one of the standard textbooks for the field. The Fifth Edition has two new authors: Jeremy Berg, Professor and Director of Biophysics and Biophysical Chemistry at Johns Hopkins University School of Medicine; and John Tymoczko, the Towsley Professor of Biology at Carleton College. The new edition does,…

  16. New Edition of Chinese Biochemistry Textbook.

    ERIC Educational Resources Information Center

    Jian-Chuan, Ma

    1988-01-01

    Discusses the four previous editions of the biochemistry medical textbooks called the "Nationwide Unified Textbooks." Notes the new (1989) edition is much smaller, is organized differently, has new material, has a reorganized Dynamic Biochemistry core, and shows great importance to clinical biochemistry. (MVL)

  17. Metabolism and biochemistry in hypogravity

    NASA Technical Reports Server (NTRS)

    Leach, Carolyn S.

    1991-01-01

    The headward shift of body fluid and increase in stress-related hormones that occur in hypogravity bring about a number of changes in metabolism and biochemistry of the human body. Such alterations may have important effects on health during flight and during a recovery period after return to earth. Body fluid and electrolytes are lost, and blood levels of several hormones that control metabolism are altered during space flight. Increased serum calcium may lead to an increased risk of renal stone formation during flight, and altered drug metabolism could influence the efficacy of therapeutic agents. Orthostatic intolerance and an increased risk of fracturing weakened bones are concerns at landing. It is important to understand biochemistry and metabolism in hypogravity so that clinically important developments can be anticipated and prevented or ameliorated.

  18. Clinical biochemistry education in Spain.

    PubMed

    Queraltó, J M

    1994-12-31

    Clinical biochemistry in Spain was first established in 1978 as an independent specialty. It is one of several clinical laboratory sciences specialties, together with haematology, microbiology, immunology and general laboratory (Clinical analysis, análisis clinicos). Graduates in Medicine, Pharmacy, Chemistry and Biological Sciences can enter post-graduate training in Clinical Chemistry after a nation-wide examination. Training in an accredited Clinical Chemistry department is 4 years. A national committee for medical and pharmacist specialties advises the government on the number of trainees, program and educational units accreditation criteria. Technical staff includes nurses and specifically trained technologists. Accreditation of laboratories is developed at different regional levels. The Spanish Society for Clinical Biochemistry and Molecular Pathology (SECQ), the national representative in the IFCC, has 1600 members, currently publishes a scientific journal (Química Clinica) and a newsletter. It organizes a continuous education program, a quality control program and an annual Congress.

  19. Air

    MedlinePlus

    ... do to protect yourself from dirty air . Indoor air pollution and outdoor air pollution Air can be polluted indoors and it can ... this chart to see what things cause indoor air pollution and what things cause outdoor air pollution! Indoor ...

  20. Gender Performance Differences in Biochemistry

    ERIC Educational Resources Information Center

    Rauschenberger, Matthew M.; Sweeder, Ryan D.

    2010-01-01

    This study examined the historical performance of students at Michigan State University in a two-part biochemistry series Biochem I (n = 5,900) and Biochem II (n = 5,214) for students enrolled from 1997 to 2009. Multiple linear regressions predicted 54.9-87.5% of the variance in student from Biochem I grade and 53.8-76.1% of the variance in…

  1. Genesis of "Biochemistry: A Problems Approach"

    ERIC Educational Resources Information Center

    Wood, William B.

    2002-01-01

    When the author began teaching as a young assistant professor at Caltech in 1966, his assignment was to take over the undergraduate biochemistry course taught for many years by Henry Borsook, who was about to retire. Students dreaded this course. Having delighted in biochemistry during his graduate training at Stanford, he was determined to put…

  2. Pedro Gutierrez Bueno's Textbooks: Audiences, Teaching Practices and Chemical Revolution

    ERIC Educational Resources Information Center

    Sanchez, Jose Ramon Bertomeu; Belmar, Antonio Garcia

    2006-01-01

    Pedro Gutierrez Bueno wrote two editions of a chemistry textbook between 1788 and 1802. The paper offers a comparative view of both editions taking into account Gutierrez Bueno's biography, his intended audience and the changes related to the so-called chemical revolution. Some conclusions are at odds with common images about scientific…

  3. Searching for Alien Life Having Unearthly Biochemistry

    NASA Technical Reports Server (NTRS)

    Jones, Harry

    2003-01-01

    The search for alien life in the solar system should include exploring unearth-like environments for life having an unearthly biochemistry. We expect alien life to conform to the same basic chemical and ecological constraints as terrestrial life, since inorganic chemistry and the laws of ecosystems appear to be universal. Astrobiologists usually assume alien life will use familiar terrestrial biochemistry and therefore hope to find alien life by searching near water or by supplying hydrocarbons. The assumption that alien life is likely to be based on carbon and water is traditional and plausible. It justifies high priority for missions to search for alien life on Mars and Europa, but it unduly restricts the search for alien life. Terrestrial carbon-water biochemistry is not possible on most of the bodies of our solar system, but all alien life is not necessarily based on terrestrial biochemistry. If alien life has a separate origin from Earth life, and if can survive in an environment extremely different from Earth's, then alien life may have unearthly biochemistry. There may be other solvents than water that support alien life and other elements than carbon that form complex life enabling chain molecules. Rather than making the exploration-restricting assumption that all life requires carbon, water, and terrestrial biochemistry, we should make the exploration-friendly assumption that indigenous, environmentally adapted, alien life forms might flourish using unearthly biochemistry in many places in the solar system. Alien life might be found wherever there is free energy and a physical/chemical system capable of using that energy to build living structures. Alien life may be discovered by the detection of some general non-equilibrium chemistry rather than of terrestrial biochemistry. We should explore all the potential abodes of life in the solar system, including those where life based on terrestrial biochemistry can not exist.

  4. A Project-Oriented Biochemistry Laboratory Course.

    ERIC Educational Resources Information Center

    Craig, Paul A.

    1999-01-01

    Describes a biochemistry laboratory course in which the curriculum revolves around a single theme: the purification, characterization, and molecular biology of threonine dehydrogenase (TDH) from Escherechia coli. Lists examples of related class research projects. Contains 41 references. (WRM)

  5. Annual review of biochemistry. Volume 59

    SciTech Connect

    Richardson, C.C. ); Abelson, J.N. ); Meister, A. ); Walsh, C.T. )

    1990-01-01

    This book contains articles reviewing significant developments in the field of biochemistry. Topics covered include: How to Succeed in Research Without Being a Genius, Pgruvagl-Dependent Enzymes, and Phytochelatins.

  6. Useful Demonstrations for a Medial Biochemistry Course.

    ERIC Educational Resources Information Center

    Ragatz, Barth H.; Modrak, Gina

    1986-01-01

    Describes six demonstrations used in a medical biochemistry course. These demonstrations focus on: (1) platelet aggregometry; (2) ion-transporting antibiotics; (3) glycosylated hemoglobin; (4) molecular models; (5) serum preparation; and (6) bioluminescence. (JN)

  7. Identification of Threshold Concepts for Biochemistry

    PubMed Central

    Green, David; Lewis, Jennifer E.; Lin, Sara; Minderhout, Vicky

    2014-01-01

    Threshold concepts (TCs) are concepts that, when mastered, represent a transformed understanding of a discipline without which the learner cannot progress. We have undertaken a process involving more than 75 faculty members and 50 undergraduate students to identify a working list of TCs for biochemistry. The process of identifying TCs for biochemistry was modeled on extensive work related to TCs across a range of disciplines and included faculty workshops and student interviews. Using an iterative process, we prioritized five concepts on which to focus future development of instructional materials. Broadly defined, the concepts are steady state, biochemical pathway dynamics and regulation, the physical basis of interactions, thermodynamics of macromolecular structure formation, and free energy. The working list presented here is not intended to be exhaustive, but rather is meant to identify a subset of TCs for biochemistry for which instructional and assessment tools for undergraduate biochemistry will be developed. PMID:25185234

  8. Identification of threshold concepts for biochemistry.

    PubMed

    Loertscher, Jennifer; Green, David; Lewis, Jennifer E; Lin, Sara; Minderhout, Vicky

    2014-01-01

    Threshold concepts (TCs) are concepts that, when mastered, represent a transformed understanding of a discipline without which the learner cannot progress. We have undertaken a process involving more than 75 faculty members and 50 undergraduate students to identify a working list of TCs for biochemistry. The process of identifying TCs for biochemistry was modeled on extensive work related to TCs across a range of disciplines and included faculty workshops and student interviews. Using an iterative process, we prioritized five concepts on which to focus future development of instructional materials. Broadly defined, the concepts are steady state, biochemical pathway dynamics and regulation, the physical basis of interactions, thermodynamics of macromolecular structure formation, and free energy. The working list presented here is not intended to be exhaustive, but rather is meant to identify a subset of TCs for biochemistry for which instructional and assessment tools for undergraduate biochemistry will be developed.

  9. Education in Medical Biochemistry in Serbia

    PubMed Central

    2010-01-01

    Medical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia. Medical biochemistry laboratories and medical biochemists as a profession are part of Health Care System and are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists in Serbia dates from 1945, when Department of Medical Biochemistry was established at Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate branch was established, educating Medical Biochemists under a special program. Since 2006 the new five year undergraduate (according to Bologna Declaration) and postgraduate program of four-year specialization according to EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredits the programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Medical Biochemistry), successful completion of the profession exam at the Ministry of Health after completion of one additional year of obligatory practical training in medical laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by Serbian Chamber of Medical Biochemists.

  10. Nutritional Biochemistry of Space Flight

    NASA Technical Reports Server (NTRS)

    Smith, Scott M.

    2000-01-01

    Adequate nutrition is critical for maintenance of crew health during and after extended-duration space flight. The impact of weightlessness on human physiology is profound, with effects on many systems related to nutrition, including bone, muscle, hematology, fluid and electrolyte regulation. Additionally, we have much to learn regarding the impact of weightlessness on absorption, mtabolism , and excretion of nutrients, and this will ultimately determine the nutrient requirements for extended-duration space flight. Existing nutritional requirements for extended-duration space flight have been formulated based on limited flight research, and extrapolation from ground-based research. NASA's Nutritional Biochemistry Laboratory is charged with defining the nutritional requirements for space flight. This is accomplished through both operational and research projects. A nutritional status assessment program is included operationally for all International Space Station astronauts. This medical requirement includes biochemical and dietary assessments, and is completed before, during, and after the missions. This program will provide information about crew health and nutritional status, and will also provide assessments of countermeasure efficacy. Ongoing research projects include studies of calcium and bone metabolism, and iron absorption and metabolism. The calcium studies include measurements of endocrine regulation of calcium homeostasis, biochemical marker of bone metabolism, and tracer kinetic studies of calcium movement in the body. These calcium kinetic studies allow for estimation of intestinal absorption, urinary excretion, and perhaps most importantly - deposition and resorption of calcium from bone. The Calcium Kinetics experiment is currently being prepared for flight on the Space Shuttle in 2001, and potentially for subsequent Shuttle and International Space Station missions. The iron study is intended to assess whether iron absorption is down-regulated dUl1ng

  11. The Biochemistry Tetrahedron and the Development of the Taxonomy of Biochemistry External Representations (TOBER)

    ERIC Educational Resources Information Center

    Towns, Marcy H.; Raker, Jeffrey R.; Becker, Nicole; Harle, Marissa; Sutcliffe, Jonathan

    2012-01-01

    Visual literacy, the ability to interpret and create external representations (ERs), is essential to success in biochemistry. Studies have been conducted that describe students' abilities to use and interpret specific types of ERs. However, a framework for describing ERs derived through a naturalistic inquiry of biochemistry classrooms has not…

  12. Glycoprotein Biochemistry--Some Clinical Aspects of Interest to Biochemistry Students.

    ERIC Educational Resources Information Center

    Smith, Christopher A.; And Others

    1991-01-01

    Authors describe some clinical features of glycoprotein biochemistry, including recognition, selected blood glycoproteins, glycated proteins, histochemistry, and cancer. The material presented has largely been taught to medical laboratory students; however, it can be used to teach premedical students and pure biochemistry students. Includes two…

  13. Absolute Interrogative Intonation Patterns in Buenos Aires Spanish

    ERIC Educational Resources Information Center

    Lee, Su Ar

    2010-01-01

    In Spanish, each uttered phrase, depending on its use, has one of a variety of intonation patterns. For example, a phrase such as "Maria viene manana" "Mary is coming tomorrow" can be used as a declarative or as an absolute interrogative (a yes/no question) depending on the intonation pattern that a speaker produces. Patterns of usage also…

  14. Parental Choice in the Buenos Aires Bilingual School Market

    ERIC Educational Resources Information Center

    Potter, Diana; Hayden, Mary

    2004-01-01

    The issue of parental choice of school for their child has been a topical one in many contexts worldwide, and work has been undertaken in a number of national contexts to investigate the many factors at work in influencing such choice. This article considers parental choice within the specific context of bilingual (Spanish/English) schools in…

  15. [Prevention of congenital toxoplasmosis in a Buenos Aires hospital].

    PubMed

    Carral, Liliana; Kaufer, Federico; Olejnik, Patricia; Freuler, Cristina; Durlach, Ricardo

    2013-01-01

    The prevention of congenital toxoplasmosis is based on providing information to women, serologic diagnosis and treatment of the infected mother and child. In this article we present the results of 12 years of implementation of a congenital toxoplasmosis prevention program in which we measured the mother's infection incidence rate, the transmission rate and the number and severity of infection in newborns. The study was performed on 12035 pregnant women in the period 2000-2011. The prevalence rate of antibodies against Toxoplasma gondii was 18.33% (2206/12035). Thirty-seven out of 9792 susceptible women presented acute infection and the mother's infection incidence rate was 3.78 per 1000 births. The transplacental transmission rate was 5.4% (2/37). Two newborns presented congenital toxoplasmosis infection, one had no clinical signs while the other presented strabismus and chorioretinitis. Thirty-five infected mothers and the two children with congenital infection were treated. The transmission rates obtained allow consider this prevention program as a valid resource to minimize the impact of congenital toxoplasmosis.

  16. BOREAS TE-9 NSA Canopy Biochemistry

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Curd, Shelaine (Editor); Margolis, Hank; Charest, Martin; Sy, Mikailou

    2000-01-01

    The BOREAS TE-9 team collected several data sets related to chemical and photosynthetic properties of leaves. This data set contains canopy biochemistry data collected in 1994 in the NSA at the YJP, OJR, OBS, UBS, and OA sites, including biochemistry lignin, nitrogen, cellulose, starch, and fiber concentrations. These data were collected to study the spatial and temporal changes in the canopy biochemistry of boreal forest cover types and how a high-resolution radiative transfer model in the mid-infrared could be applied in an effort to obtain better estimates of canopy biochemical properties using remote sensing. The data are available in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

  17. Electrospray and tandem mass spectrometry in biochemistry.

    PubMed Central

    Griffiths, W J; Jonsson, A P; Liu, S; Rai, D K; Wang, Y

    2001-01-01

    Over the last 20 years, biological MS has changed out of all recognition. This is primarily due to the development in the 1980s of 'soft ionization' methods that permit the ionization and vaporization of large, polar, and thermally labile biomolecules. These developments in ionization mode have driven the design and manufacture of smaller and cheaper mass analysers, making the mass spectrometer a routine instrument in the biochemistry laboratory today. In the present review the revolutionary 'soft ionization' methods will be discussed with particular reference to electrospray. The mass analysis of ions will be described, and the concept of tandem MS introduced. Where appropriate, examples of the application of MS in biochemistry will be provided. Although the present review will concentrate on the MS of peptides/proteins and lipids, all classes of biomolecules can be analysed, and much excellent work has been done in the fields of carbohydrate and nucleic acid biochemistry. PMID:11311115

  18. Commentary: PhDs in Biochemistry Education--5 Years Later

    ERIC Educational Resources Information Center

    Offerdahl, Erika G.; Momsen, Jennifer L.; Osgood, Marcy

    2014-01-01

    In this commentary, the discussion of PhDs in biochemistry education research is expanded to explore a number of diverse pathways leading to a competitive research program in biochemistry education research.

  19. Commentary: PhDs in biochemistry education-5 years later.

    PubMed

    Offerdahl, Erika G; Momsen, Jennifer L; Osgood, Marcy

    2014-01-01

    In this commentary, the discussion of PhDs in biochemistry education research is expanded to explore a number of diverse pathways leading to a competitive research program in biochemistry education research.

  20. A Kinetic Experiment for the Biochemistry Laboratory.

    ERIC Educational Resources Information Center

    Palmer, Richard E.

    1986-01-01

    Discusses the use of specific reactions of metabolic pathways to make measurements in the laboratory. Describes an adaptation of an experiment used in undergraduate biochemistry laboratories involving the induction of an enzyme in E. coli, as well as its partial purification and characterization. (TW)

  1. Identification of Threshold Concepts for Biochemistry

    ERIC Educational Resources Information Center

    Loertscher, Jennifer; Green, David; Lewis, Jennifer E.; Lin, Sara; Minderhout, Vicky

    2014-01-01

    Threshold concepts (TCs) are concepts that, when mastered, represent a transformed understanding of a discipline without which the learner cannot progress. We have undertaken a process involving more than 75 faculty members and 50 undergraduate students to identify a working list of TCs for biochemistry. The process of identifying TCs for…

  2. Teaching Receptor Theory to Biochemistry Undergraduates

    ERIC Educational Resources Information Center

    Benore-Parsons, Marilee; Sufka, Kenneth J.

    2003-01-01

    Receptor:ligand interactions account for numerous reactions critical to biochemistry and molecular biology. While students are typically exposed to some examples, such as hemoglobin binding of oxygen and signal transduction pathways, the topic could easily be expanded. Theory and kinetic analysis, types of receptors, and the experimental assay…

  3. Rhetorical Structure of Biochemistry Research Articles

    ERIC Educational Resources Information Center

    Kanoksilapatham, Budsaba

    2005-01-01

    This paper reports on the results of a move analysis [Swales, J. (1990). "Genre analysis." Cambridge: Cambridge University Press] of 60 biochemistry research articles. First, a corpus was systematically compiled to ensure that it represents core journals in the focused discipline. Then, coding reliability analysis was conducted to demonstrate…

  4. The Physiology and Biochemistry of Receptors.

    ERIC Educational Resources Information Center

    Spitzer, Judy A., Ed.

    1983-01-01

    The syllabus for a refresher course on the physiology and biochemistry of receptors (presented at the 1983 American Physiological Society meeting) is provided. Topics considered include receptor regulation, structural/functional aspects of receptors for insulin and insulin-like growth factors, calcium channel inhibitors, and role of lipoprotein…

  5. Jmol-Enhanced Biochemistry Research Projects

    ERIC Educational Resources Information Center

    Saderholm, Matthew; Reynolds, Anthony

    2011-01-01

    We developed a protein research project for a one-semester biochemistry lecture class to enhance learning and more effectively train students to understand protein structure and function. During this semester-long process, students select a protein with known structure and then research its structure, sequence, and function. This project…

  6. Television Medical Dramas as Case Studies in Biochemistry

    ERIC Educational Resources Information Center

    Millard, Julie T.

    2009-01-01

    Several case studies from popular television medical dramas are described for use in an undergraduate biochemistry course. These cases, which illustrate fundamental principles of biochemistry, are used as the basis for problems that can be discussed further in small groups. Medical cases provide an interesting context for biochemistry with video…

  7. A Biochemistry and Molecular Biology Course for Secondary School Teachers

    ERIC Educational Resources Information Center

    Fernandez-Novell, J. M.; Cid, E.; Gomis, R.; Barbera, A.; Guinovart, J. J.

    2004-01-01

    This article describes a course for reinforcing the knowledge of biochemistry in secondary school science teachers. The Department of Biochemistry and Molecular Biology of the University of Barcelona designed a course to bring these teachers up to date with this discipline. In addition to updating their knowledge of biochemistry and molecular…

  8. Teachers as Learners in a Cooperative Learning Biochemistry Class

    ERIC Educational Resources Information Center

    Osgood, Marcy P.; Mitchell, Steve M.; Anderson, William L.

    2005-01-01

    Upper level college students majoring in biochemistry at the University of New Mexico have the opportunity to participate in an advanced biochemistry course entitled "Biochemistry Education." This course introduces theories of teaching and learning, provides opportunities for participation in course organization, design, and assessment strategies,…

  9. Biochemistry and genetics of starch synthesis.

    PubMed

    Keeling, Peter L; Myers, Alan M

    2010-01-01

    Enormous progress has been made in understanding the genetics and biochemistry of starch synthesis in crop plants. Furthermore, starch remains at the very epicenter of the world's food and feed chains and has even now become one of the world's most important sources of biorenewable energy (biofuel). Yet, despite this remarkable progress and the obvious economic importance, very little has been achieved in terms of adding value to starch or increasing starch yield, particularly in cereal crops. Here, we review the genetics and biochemistry of starch synthesis in crop plants, particularly maize. With all this know-how in place and a chasm of opportunity ahead, the time is right to see science deliver progress into a new frontier. Thus, in our view the stage is set for a new era of changes in starch synthesis, delivering enhancements in functionality and yield.

  10. A biochemistry and molecular biology course for secondary school teachers*.

    PubMed

    Fernández-Novell, J M; Cid, E; Gomis, R; Barberà, A; Guinovart, J J

    2004-11-01

    This article describes a course for reinforcing the knowledge of biochemistry in secondary school science teachers. The Department of Biochemistry and Molecular Biology of the University of Barcelona designed a course to bring these teachers up to date with this discipline. In addition to updating their knowledge of biochemistry and molecular biology, this course aims to provide teachers with a set of relevant laboratory practices that can be applied in their practical lessons.

  11. Writing throughout the Biochemistry Curriculum: Synergistic Inquiry-Based Writing Projects for Biochemistry Students

    ERIC Educational Resources Information Center

    Mertz, Pamela; Streu, Craig

    2015-01-01

    This article describes a synergistic two-semester writing sequence for biochemistry courses. In the first semester, students select a putative protein and are tasked with researching their protein largely through bioinformatics resources. In the second semester, students develop original ideas and present them in the form of a research grant…

  12. In-Cell Biochemistry Using NMR Spectroscopy

    PubMed Central

    Burz, David S.; Shekhtman, Alexander

    2008-01-01

    Biochemistry and structural biology are undergoing a dramatic revolution. Until now, mostly in vitro techniques have been used to study subtle and complex biological processes under conditions usually remote from those existing in the cell. We developed a novel in-cell methodology to post-translationally modify interactor proteins and identify the amino acids that comprise the interaction surface of a target protein when bound to the post-translationally modified interactors. Modifying the interactor proteins causes structural changes that manifest themselves on the interacting surface of the target protein and these changes are monitored using in-cell NMR. We show how Ubiquitin interacts with phosphorylated and non-phosphorylated components of the receptor tyrosine kinase (RTK) endocytic sorting machinery: STAM2 (Signal-transducing adaptor molecule), Hrs (Hepatocyte growth factor regulated substrate) and the STAM2-Hrs heterodimer. Ubiquitin binding mediates the processivity of a large network of interactions required for proper functioning of the RTK sorting machinery. The results are consistent with a weakening of the network of interactions when the interactor proteins are phosphorylated. The methodology can be applied to any stable target molecule and may be extended to include other post-translational modifications such as ubiquitination or sumoylation, thus providing a long-awaited leap to high resolution in cell biochemistry. PMID:18626516

  13. Enhanced Podcasts for Teaching Biochemistry to Veterinary Students

    ERIC Educational Resources Information Center

    Gough, Kevin C.

    2011-01-01

    The teaching of biochemistry within medical disciplines presents certain challenges; firstly to relay a large body of complex facts and abstract concepts, and secondly to motivate students that this relatively difficult topic is worth their time to study. Here, nutrient biochemistry was taught within a multidisciplinary module as part of an…

  14. A Streamlined Molecular Biology Module for Undergraduate Biochemistry Labs

    ERIC Educational Resources Information Center

    Muth, Gregory W.; Chihade, Joseph W.

    2008-01-01

    Site-directed mutagenesis and other molecular biology techniques, including plasmid manipulation and restriction analysis, are commonly used tools in the biochemistry research laboratory. In redesigning our biochemistry lab curricula, we sought to integrate these techniques into a term-long, project-based course. In the module presented here,…

  15. Using Pamphlets to Teach Biochemistry: A Service-Learning Project

    ERIC Educational Resources Information Center

    Harrison, Melinda A.; Dunbar, David; Lopatto, David

    2013-01-01

    A service-learning project appropriate for a biochemistry or advanced biochemistry course was designed and implemented. The project involved students partnering with a homeless shelter to design informational pamphlets to be displayed at the shelter for the clients' use. The pamphlet topics were based on diseases studied within the course.…

  16. Commentary: Biochemistry and Molecular Biology Educators Launch National Network

    ERIC Educational Resources Information Center

    Bailey, Cheryl; Bell, Ellis; Johnson, Margaret; Mattos, Carla; Sears, Duane; White, Harold B.

    2010-01-01

    The American Society of Biochemistry and Molecular Biology (ASBMB) has launched an National Science Foundation (NSF)-funded 5 year project to support biochemistry and molecular biology educators learning what and how students learn. As a part of this initiative, hundreds of life scientists will plan and develop a rich central resource for…

  17. Blended Learning in Biochemistry Education: Analysis of Medical Students' Perceptions

    ERIC Educational Resources Information Center

    Wardenski, Rosilaine de Fatima; de Espindola, Marina Bazzo; Struchiner, Miriam; Giannella, Tais Rabetti

    2012-01-01

    The objective of this study was to analyze first-year UFRJ medical students' perceptions about the implementation of a blended learning (BL) experience in their Biochemistry I course. During the first semester of 2009, three Biochemistry professors used the Constructore course management system to develop virtual learning environments (VLEs) for…

  18. The Biochemistry of Bereavement: Possible Basis for Chemotherapy?

    ERIC Educational Resources Information Center

    Fredrick, Jerome F.

    1982-01-01

    Reviews the physiological effect of acute grief and explores the increased susceptibility to infectious disease agents in terms of the altered biochemistry of the bereaved individual. Until basic reactions of grief are defined and the altered biochemistry established, psychological methods appear to offer the best therapy. (Author/JAC)

  19. Immobilized alpha-Galactosidase in the Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Mulimani, V. H.; Dhananjay, K.

    2007-01-01

    This laboratory experiment was designed to demonstrate the application of immobilized galactosidase in food industry to hydrolyze raffinose family oligosaccharides in soymilk. This laboratory experiment was conducted for postgraduate students of biochemistry and developed for graduate and undergraduate students of biochemistry, biotechnology,…

  20. Teachers as learners in a cooperative learning biochemistry class.

    PubMed

    Osgood, Marcy P; Mitchell, Steve M; Anderson, William L

    2005-11-01

    Upper level college students majoring in biochemistry at the University of New Mexico have the opportunity to participate in an advanced biochemistry course entitled "Biochemistry Education." This course introduces theories of teaching and learning, provides opportunities for participation in course organization, design, and assessment strategies, and requires practice in lecturing, exam writing, and grading. One component of this course required the biochemistry majors to act as educational assistants, leading problem-based learning sessions in a cooperative learning introductory survey biochemistry course for nonmajors. Problem-based learning scenarios used in this course were based on real-life biochemistry problems. As a result of their participation, the educational assistants increased their understanding of the biochemistry principles, gained an appreciation for the difficulty of the job of a "good teacher," developed new approaches to their own learning, and became more confident speakers. The participating biochemistry faculty were also positively affected by the collaborative approach they were attempting to model for the two sets of students and realized the benefits of truly cooperative team teaching.

  1. A National Comparison of Biochemistry and Molecular Biology Capstone Experiences

    ERIC Educational Resources Information Center

    Aguanno, Ann; Mertz, Pamela; Martin, Debra; Bell, Ellis

    2015-01-01

    Recognizing the increasingly integrative nature of the molecular life sciences, the "American Society for Biochemistry and Molecular Biology" (ASBMB) recommends that Biochemistry and Molecular Biology (BMB) programs develop curricula based on concepts, content, topics, and expected student outcomes, rather than courses. To that end,…

  2. Incorporation of Bioinformatics Exercises into the Undergraduate Biochemistry Curriculum

    ERIC Educational Resources Information Center

    Feig, Andrew L.; Jabri, Evelyn

    2002-01-01

    The field of bioinformatics is developing faster than most biochemistry textbooks can adapt. Supplementing the undergraduate biochemistry curriculum with data-mining exercises is an ideal way to expose the students to the common databases and tools that take advantage of this vast repository of biochemical information. An integrated collection of…

  3. Immobilized Lactase in the Biochemistry Laboratory

    NASA Astrophysics Data System (ADS)

    Allison, Matthew J.; Bering, C. Larry

    1998-10-01

    Immobilized enzymes have many practical applications. They may be used in clinical, industrial, and biotechnological laboratories and in many clinical diagnostic kits. For educational purposes, use of immobilized enzymes can easily be taught at the undergraduate or even secondary level. We have developed an immobilized enzyme experiment that combines many practical techniques used in the biochemistry laboratory and fits within a three-hour time frame. In this experiment, lactase from over-the-counter tablets for patients with lactose intolerance is immobilized in polyacrylamide, which is then milled into small beads and placed into a chromatography column. A lactose solution is added to the column and the eluant is assayed using the glucose oxidase assay, available as a kit. We have determined the optimal conditions to give the greatest turnover of lactose while allowing the immobilized enzymes to be active for long periods at room temperature.

  4. Bone Biochemistry on the International Space Station

    NASA Technical Reports Server (NTRS)

    Smith, Scott M.; Heer, Martina; Zwart, Sara R.

    2016-01-01

    Bone biochemical measures provide valuable insight into the nature and time course of microgravity effects on bone during space flight, where imaging technology cannot be employed. Increased bone resorption is a hallmark of space flight, while markers of bone formation are typically unchanged or decreased. Recent studies (after the deployment to ISS of the advanced resistive exercise device, ARED), have documented that astronauts with good nutritional intake (e.g., maintenance of body mass), good vitamin D status, and exercise maintained bone mineral density. These data are encouraging, but crewmembers exercising on the ARED do have alterations in bone biochemistry, specifically, bone resorption is still increased above preflight levels, but bone formation is also significantly increased. While this bone remodeling raises questions about the strength of the resulting bone, however documents beneficial effects of nutrition and exercise in counteracting bone loss of space flight.

  5. Role of tautomerism in RNA biochemistry.

    PubMed

    Singh, Vipender; Fedeles, Bogdan I; Essigmann, John M

    2015-01-01

    Heterocyclic nucleic acid bases and their analogs can adopt multiple tautomeric forms due to the presence of multiple solvent-exchangeable protons. In DNA, spontaneous formation of minor tautomers has been speculated to contribute to mutagenic mispairings during DNA replication, whereas in RNA, minor tautomeric forms have been proposed to enhance the structural and functional diversity of RNA enzymes and aptamers. This review summarizes the role of tautomerism in RNA biochemistry, specifically focusing on the role of tautomerism in catalysis of small self-cleaving ribozymes and recognition of ligand analogs by riboswitches. Considering that the presence of multiple tautomers of nucleic acid bases is a rare occurrence, and that tautomers typically interconvert on a fast time scale, methods for studying rapid tautomerism in the context of nucleic acids under biologically relevant aqueous conditions are also discussed.

  6. Differentiating biochemistry course laboratories based on student experience.

    PubMed

    Jakubowski, Henry V

    2011-01-01

    Content and emphases in undergraduate biochemistry courses can be readily tailored to accommodate the standards of the department in which they are housed, as well as the backgrounds of the students in the courses. A more challenging issue is how to construct laboratory experiences for a class with both chemistry majors, who usually have little or no experience with biochemical techniques and biology and biochemistry majors who do. This manuscript describes a strategy for differentiating biochemistry labs to meet the needs of students with differing backgrounds.

  7. Current topics in plant biochemistry and physiology: Volume 4: Proceedings

    SciTech Connect

    Randall, D.D.; Blevins, D.G.; Larson, R.L.

    1985-01-01

    These proceedings represent papers presented at the Fourth Annual Plant Biochemistry and Physiology Symposium held at the University of Missouri-Columbia, April 10-12, 1985 and hosted by the Interdisciplinary Plant Biochemistry and Physiology Group. This Interdisciplinary Group was organized to facilitate research and training through interdisciplinary and cooperative approaches to problems facing Plant Biochemistry and Physiology. A key objective of this group is to maintain an awareness of the advances in research in this field. This annual symposium is one means of meeting this objective. Topics are selected each year in three to five areas in order to broaden our individual horizons.

  8. An Integrated Biochemistry Laboratory, Including Molecular Modeling

    NASA Astrophysics Data System (ADS)

    Hall, Adele J. Wolfson Mona L.; Branham, Thomas R.

    1996-11-01

    The dilemma of designing an advanced undergraduate laboratory lies in the desire to teach and reinforce basic principles and techniques while at the same time exposing students to the excitement of research. We report here on a one-semester, project-based biochemistry laboratory that combines the best features of a cookbook approach (high success rate, achievement of defined goals) with those of an investigative, discovery-based approach (student involvement in the experimental design, excitement of real research). Individual modules may be selected and combined to meet the needs of different courses and different institutions. The central theme of this lab is protein purification and design. This laboratory accompanies the first semester of biochemistry (Structure and Function of Macromolecules, a course taken mainly by junior and senior chemistry and biological chemistry majors). The protein chosen as the object of study is the enzyme lysozyme, which is utilized in all projects. It is suitable for a student lab because it is easily and inexpensively obtained from egg white and is extremely stable, and its high isoelectric point (pI = 11) allows for efficient separation from other proteins by ion-exchange chromatography. Furthermore, a literature search conducted by the resourceful student reveals a wealth of information, since lysozyme has been the subject of numerous studies. It was the first enzyme whose structure was determined by crystallography (1). Hendrickson et al. (2) have previously described an intensive one-month laboratory course centered around lysozyme, although their emphasis is on protein stability rather than purification and engineering. Lysozyme continues to be the focus of much exciting new work on protein folding and dynamics, structure and activity (3 - 5). This lab course includes the following features: (i) reinforcement of basic techniques, such as preparation of buffers, simple enzyme kinetics, and absorption spectroscopy; (ii

  9. Rembrandt’s Jewish Physician—Dr Ephraim Bueno (1599–1665): A Brief Medical History

    PubMed Central

    Weisz, George M.; Albury, William R.

    2013-01-01

    Medicine in the Middle Ages was, and ever since remained, one of the main preoccupations of the professionally restricted Jews. One of the medical dynasties on the Iberian Peninsula was the Bueno (Bonus) family. Following the expulsion of the Jews from Spain and their spread in Europe, these Iberian physicians became successful everywhere—just as the Buenos were in the Netherlands. PMID:23908860

  10. Curriculum Guidelines on Biochemistry and Nutrition for Dental Hygienists.

    ERIC Educational Resources Information Center

    Journal of Dental Education, 1984

    1984-01-01

    Guidelines developed by the Sections of Biochemistry and Nutrition and Dental Hygiene Education of the American Association of Dental Schools are intended for use by individual educational institutions as curriculum development aids. (MLW)

  11. International Environmental Law and Biochemistry: An Innovative Teaching Opportunity.

    ERIC Educational Resources Information Center

    Candlish, John

    1998-01-01

    Explores the ties between international environmental law and biochemistry with respect to genetically modified organisms, biodiversity, marine pollution, cancer biology, and pesticide contamination of food. Contains 30 references. (DDR)

  12. A Continuing Education Project for Updating Women in Biochemistry.

    ERIC Educational Resources Information Center

    Mary Kieran, McElroy, Sister

    1978-01-01

    Describes an interdisciplinary program in the areas of biochemistry, biology, and computer science offered at Chestnut Hill College, Philadelphia, Pennsylvania, to update 30 women in order to prepare them to return to the job market in chemistry. (HM)

  13. One hundred years of American Women in biochemistry *.

    PubMed

    Wolfson, Adele J

    2006-03-01

    The occasion of ASBMB's 100th anniversary provides an opportunity to consider the history and accomplishments of women biochemists over the past century. American women in biochemistry have ranged from the highly visible, such as Nobel Prize winners Gert Cori and Gertrude Elion, to those who contributed in relative obscurity to equally dramatic breakthroughs. Women with Ph.D.s in biochemistry established departments of public health, nutrition, and home economics when more prestigious academic departments were not open to them. As in all fields of science, women have made great progress in biochemistry in recent years, and ASBMB reflects that progress; the past, current, and incoming presidents of the Society are all women. The data and biographical information presented here should provide a basis for inclusion of more women in recounting the history of biochemistry. PMID:21638642

  14. Can biochemistry drive drug discovery beyond simple potency measurements?

    PubMed

    Chène, Patrick

    2012-04-01

    Among the fields of expertise required to develop drugs successfully, biochemistry holds a key position in drug discovery at the interface between chemistry, structural biology and cell biology. However, taking the example of protein kinases, it appears that biochemical assays are mostly used in the pharmaceutical industry to measure compound potency and/or selectivity. This limited use of biochemistry is surprising, given that detailed biochemical analyses are commonly used in academia to unravel molecular recognition processes. In this article, I show that biochemistry can provide invaluable information on the dynamics and energetics of compound-target interactions that cannot be obtained on the basis of potency measurements and structural data. Therefore, an extensive use of biochemistry in drug discovery could facilitate the identification and/or development of new drugs.

  15. An integrated strategy for teaching biochemistry to biotechnology specialty students.

    PubMed

    Ouyang, Liming; Ou, Ling; Zhang, Yuanxing

    2007-07-01

    The faculty of biochemistry established an integrated teaching strategy for biotechnology specialty students, by intermeshing the case-study method, web-assistant teaching, and improved lecture format with a brief content and multimedia courseware. Teaching practice showed that the integrated teaching strategy could retain the best features of each pedagogy and better solve the main difficulties that lay in the teaching of biochemistry to biotechnology specialty students in the East China University of Science and Technology. PMID:21591104

  16. Writing throughout the biochemistry curriculum: Synergistic inquiry-based writing projects for biochemistry students.

    PubMed

    Mertz, Pamela; Streu, Craig

    2015-01-01

    This article describes a synergistic two-semester writing sequence for biochemistry courses. In the first semester, students select a putative protein and are tasked with researching their protein largely through bioinformatics resources. In the second semester, students develop original ideas and present them in the form of a research grant proposal. Both projects involve multiple drafts and peer review. The complementarity of the projects increases student exposure to bioinformatics and literature resources, fosters higher-order thinking skills, and develops teamwork and communication skills. Student feedback and responses on perception surveys demonstrated that the students viewed both projects as favorable learning experiences.

  17. Can Polyphosphate Biochemistry Affect Biological Apatite Saturation?

    NASA Astrophysics Data System (ADS)

    Omelon, S. J.; Matsuura, N.; Gorelikov, I.; Wynnyckyj, C.; Grynpas, M. D.

    2010-12-01

    Phosphorus (P) is an important and limiting element for life. One strategy for storing ortho phosphates (Pi) is polymerization. Polymerized Pi's (polyphosphates: (PO3-)n: polyPs) serve as a Pi bank, as well as a catiion chelator, energy source, & regulator of responses to stresses in the stationary phase of culture growth and development1. PolyP biochemistry has been investigated in yeasts, bacteria & plants2. Bigeochemical cycling of P includes the condensation of Pi into pyro (P2O7-4), & polyPs, & the release of Pi from these compounds by the hydrolytic degradation of Pi from phosphomonoester bonds. Alkaline phosphatase (ALP) is one of the predominate enzymes for regenerating Pi in aquatic systems3, & it cleaves Pi from polyPs. ALP is also the enzyme associated with apatite biomineralization in vertebrates4. PolyP was proposed to be the ALP substrate in bone mineralization5. Where calcium ions are plentiful in many aquatic environments, there is no requirement for aquatic life to generate Ca-stores. However, terrestrial vertebrates benefit from a bioavailable Ca-store such as apatite. The Pi storage strategy of polymerizing PO4-3 into polyPs dovetails well with Ca-banking, as polyPs sequester Ca, forming a neutral calcium polyphosphate (Ca-polyP: (Ca(PO3)2)n) complex. This neutral complex represents a high total [Ca+2] & [PO4-3], without the threat of inadvertent apatite precipitation, as the free [Ca+2] & [PO4-3], and therefore apatite saturation, are zero. Recent identification of polyP in regions of bone resorption & calcifying cartilage5 suggests that vertebrates may use polyP chemistry to bank Ca+2 and PO4-3. In vitro experiments with nanoparticulate Ca-polyP & ALP were undertaken to determine if carbonated apatite could precipitate from 1M Ca-polyP in Pi-free “physiological fluid” (0.1 M NaCl, 2 mM Ca+2, 0.8 mM Mg+2, pH ~8.0 ±0.5, 37 °C), as this is estimated to generate the [Ca+2] & [PO4-3] required to form the apatite content of bone tissue

  18. Geobiochemistry: Placing Biochemistry in Its Geochemical Context

    NASA Astrophysics Data System (ADS)

    Shock, E.; Boyer, G. M.; Canovas, P. A., III; Prasad, A.; Dick, J. M.

    2014-12-01

    Goals of geobiochemistry include simultaneously evaluating the relative stabilities of microbial cells and minerals, and predicting how the composition of biomolecules can change in response to the progress of geochemical reactions. Recent developments in theoretical geochemistry make it possible to predict standard thermodynamic properties of proteins, nucleotides, lipids, and many metabolites including the constituents of the citric acid cycle, at all temperatures and pressures where life is known to occur, and beyond. Combining these predictions with constraints from geochemical data makes it possible to assess the relative stabilities of biomolecules. Resulting independent predictions of the environmental occurrence of homologous proteins and lipid side-chains can be compared with observations from metagenomic and metalipidomic data to quantify geochemical driving forces that shape the composition of biomolecules. In addition, the energetic costs of generating biomolecules from within a diverse range of habitable environments can be evaluated in terms of prevailing geochemical variables. Comparisons of geochemical bioenergetic calculations across habitats leads to the generalization that the availability of H2 determines the cost of autotrophic biosynthesis relative to the aquatic environment external to microbial cells, and that pH, temperature, pressure, and availability of C, N, P, and S are typically secondary. Increasingly reduced conditions, which are determined by reactions of water with mineral surfaces and mineral assemblages, allow many biosynthetic reactions to shift from costing energy to releasing energy. Protein and lipid synthesis, as well as the reverse citric acid cycle, become energy-releasing processes under these conditions. The resulting energy balances that determine habitability contrast dramatically with assumptions derived from oxic surface conditions, such as those where human biochemistry operates.

  19. Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.

    PubMed Central

    Fetzner, S; Lingens, F

    1994-01-01

    This review is a survey of bacterial dehalogenases that catalyze the cleavage of halogen substituents from haloaromatics, haloalkanes, haloalcohols, and haloalkanoic acids. Concerning the enzymatic cleavage of the carbon-halogen bond, seven mechanisms of dehalogenation are known, namely, reductive, oxygenolytic, hydrolytic, and thiolytic dehalogenation; intramolecular nucleophilic displacement; dehydrohalogenation; and hydration. Spontaneous dehalogenation reactions may occur as a result of chemical decomposition of unstable primary products of an unassociated enzyme reaction, and fortuitous dehalogenation can result from the action of broad-specificity enzymes converting halogenated analogs of their natural substrate. Reductive dehalogenation either is catalyzed by a specific dehalogenase or may be mediated by free or enzyme-bound transition metal cofactors (porphyrins, corrins). Desulfomonile tiedjei DCB-1 couples energy conservation to a reductive dechlorination reaction. The biochemistry and genetics of oxygenolytic and hydrolytic haloaromatic dehalogenases are discussed. Concerning the haloalkanes, oxygenases, glutathione S-transferases, halidohydrolases, and dehydrohalogenases are involved in the dehalogenation of different haloalkane compounds. The epoxide-forming halohydrin hydrogen halide lyases form a distinct class of dehalogenases. The dehalogenation of alpha-halosubstituted alkanoic acids is catalyzed by halidohydrolases, which, according to their substrate and inhibitor specificity and mode of product formation, are placed into distinct mechanistic groups. beta-Halosubstituted alkanoic acids are dehalogenated by halidohydrolases acting on the coenzyme A ester of the beta-haloalkanoic acid. Microbial systems offer a versatile potential for biotechnological applications. Because of their enantiomer selectivity, some dehalogenases are used as industrial biocatalysts for the synthesis of chiral compounds. The application of dehalogenases or bacterial

  20. Toward a global approach of biochemistry in hospitals.

    PubMed

    Boigne, J M; Moisdon, J C; Tonneau, D

    1985-01-01

    This article summarizes three different presentations delivered at the CRESGE congress by three members of GREBIO, a reflection group about biochemistry; created in the Paris hospitals, this group was concerned with these problems at a time of great technological change. Physicians, biochemists and administrators have been working in GREBIO for 7 years, with the methodological assistance of management from the School of Mines; they used new approaches, and careful investigations on the field and proposed analyses sometimes upsetting but always enriching the questionings. Starting from these results, briefly described, GREBIO proposed the creation of an observatory for biochemistry, that could take into account both the complexity and diversity in the laboratories. It also proposed that current reform in analytical accountancy allow biochemistry to work its own managing tools separately from accountancy purposes.

  1. Blended learning in biochemistry education: analysis of medical students' perceptions.

    PubMed

    de Fátima Wardenski, Rosilaine; de Espíndola, Marina Bazzo; Struchiner, Miriam; Giannella, Taís Rabetti

    2012-07-01

    The objective of this study was to analyze first-year UFRJ medical students' perceptions about the implementation of a blended learning (BL) experience in their Biochemistry I course. During the first semester of 2009, three Biochemistry professors used the Constructore course management system to develop virtual learning environments (VLEs) for complementing course Modules I, II, and IV, using different resources and activities. Forty-nine students (46%) took part in the study. Results show that, in general, students gave positive evaluations to their experiences with BL, indicating that the VLEs have not only motivated but also facilitated learning. Most of the students reported that access to resources in the three modules provided a more in-depth approach to Biochemistry education and greater study autonomy. Students suggested that the VLEs could be better used for promoting greater communication among participants.

  2. My Lifelong Passion for Biochemistry and Anaerobic Microorganisms.

    PubMed

    Thauer, Rudolf Kurt

    2015-01-01

    Early parental influence led me first to medical school, but after developing a passion for biochemistry and sensing the need for a deeper foundation, I changed to chemistry. During breaks between semesters, I worked in various biochemistry labs to acquire a feeling for the different areas of investigation. The scientific puzzle that fascinated me most was the metabolism of the anaerobic bacterium Clostridium kluyveri, which I took on in 1965 in Karl Decker's lab in Freiburg, Germany. I quickly realized that little was known about the biochemistry of strict anaerobes such as clostridia, methanogens, acetogens, and sulfate-reducing bacteria and that these were ideal model organisms to study fundamental questions of energy conservation, CO2 fixation, and the evolution of metabolic pathways. My passion for anaerobes was born then and is unabated even after 50 years of study.

  3. The use of multiple tools for teaching medical biochemistry.

    PubMed

    Sé, Alexandre B; Passos, Renato M; Ono, André H; Hermes-Lima, Marcelo

    2008-03-01

    In this work, we describe the use of several strategies employing the philosophies of active learning and problem-based learning (PBL) that may be used to improve the teaching of metabolic biochemistry to medical and nutritional undergraduate students. The main activities are as follows: 1) a seminar/poster system in a mini-congress format (using topics of applied biochemistry); 2) a true/false applied biochemistry exam (written by peer tutors); 3) a 9-h exam on metabolism (based in real publications); 4) the Advanced Biochemistry course (directed to peer tutors, where students learn how to read and criticize real medical papers); 5) experiments about nutrition and metabolism, using students as volunteers, and about free radicals (real science for students); 6) the BioBio blog (taking advantage of the "web age," this enhances out of class exchanges of information between the professor, students, and peer tutors); 7) student lectures on public health issues and metabolic disorders directed to the community and lay people; and 8) the BioBio quiz show. The main objective of these activities is to provide students with a more practical and interesting approach to biochemistry, such as the application of theoretical knowledge to real situations (diseases, experiments, media information, and scientific discoveries). In addition, we emphasize the importance of peer tutor activities for optimized learning of both students and peer tutors, the importance of a closer interaction between students and teaching staff, and the necessity to initiate students precociously in two broad fields of medical activity: "real" basic science and contact with the public (also helping students--future doctors and nutritionists--to be able to communicate with lay people). Most activities were evaluated by the students through written questionnaires and informal conversations, along various semesters, indicating good acceptance and approval of these methods. Good student scores in the

  4. An Integrated Biochemistry Laboratory, Including Molecular Modeling

    NASA Astrophysics Data System (ADS)

    Hall, Adele J. Wolfson Mona L.; Branham, Thomas R.

    1996-11-01

    The dilemma of designing an advanced undergraduate laboratory lies in the desire to teach and reinforce basic principles and techniques while at the same time exposing students to the excitement of research. We report here on a one-semester, project-based biochemistry laboratory that combines the best features of a cookbook approach (high success rate, achievement of defined goals) with those of an investigative, discovery-based approach (student involvement in the experimental design, excitement of real research). Individual modules may be selected and combined to meet the needs of different courses and different institutions. The central theme of this lab is protein purification and design. This laboratory accompanies the first semester of biochemistry (Structure and Function of Macromolecules, a course taken mainly by junior and senior chemistry and biological chemistry majors). The protein chosen as the object of study is the enzyme lysozyme, which is utilized in all projects. It is suitable for a student lab because it is easily and inexpensively obtained from egg white and is extremely stable, and its high isoelectric point (pI = 11) allows for efficient separation from other proteins by ion-exchange chromatography. Furthermore, a literature search conducted by the resourceful student reveals a wealth of information, since lysozyme has been the subject of numerous studies. It was the first enzyme whose structure was determined by crystallography (1). Hendrickson et al. (2) have previously described an intensive one-month laboratory course centered around lysozyme, although their emphasis is on protein stability rather than purification and engineering. Lysozyme continues to be the focus of much exciting new work on protein folding and dynamics, structure and activity (3 - 5). This lab course includes the following features: (i) reinforcement of basic techniques, such as preparation of buffers, simple enzyme kinetics, and absorption spectroscopy; (ii

  5. Inverting Organic and Biochemistry: A Curriculum Tweak That Benefits All

    NASA Astrophysics Data System (ADS)

    Reingold, I. David

    2004-04-01

    This article makes the case for re-ordering the material currently taught in sophomore organic chemistry and junior biochemistry courses, so that most of the biochemistry appears in the sophomore course and some of the more esoteric organic chemistry material is postponed until junior year. That way, nonmajors taking organic chemistry for career purposes (premedical students, biology majors, environmental scientists) will learn chemistry more relevant to them, and chemistry majors will not suffer by having anything left out. The article also proposes details of what topics should be moved from sophomore to junior year, and vice versa.

  6. Biochemistry of nitric oxide and its redox-activated forms.

    PubMed

    Stamler, J S; Singel, D J; Loscalzo, J

    1992-12-18

    Nitric oxide (NO.), a potentially toxic molecule, has been implicated in a wide range of biological functions. Details of its biochemistry, however, remain poorly understood. The broader chemistry of nitrogen monoxide (NO) involves a redox array of species with distinctive properties and reactivities: NO+ (nitrosonium), NO., and NO- (nitroxyl anion). The integration of this chemistry with current perspectives of NO biology illuminates many aspects of NO biochemistry, including the enzymatic mechanism of synthesis, the mode of transport and targeting in biological systems, the means by which its toxicity is mitigated, and the function-regulating interaction with target proteins.

  7. A Laboratory Course in Clinical Biochemistry Emphasizing Interest and Relevance

    ERIC Educational Resources Information Center

    Schwartz, Peter L.

    1975-01-01

    Ten laboratory experiments are described which are used in a successful clinical biochemistry laboratory course (e.g. blood alcohol, glucose tolerance, plasma triglycerides, coronary risk index, gastric analysis, vitamin C and E). Most of the experiments are performed on the students themselves using simple equipment with emphasis on useful…

  8. Using Assessment to Improve Learning in the Biochemistry Classroom

    ERIC Educational Resources Information Center

    Loertscher, Jennifer

    2010-01-01

    In recent years, major drivers of undergraduate science education reform including the National Science Foundation (NSF) and the Howard Hughes Medical Institute (HHMI) have called on college and university instructors to take a more scientific approach to their teaching. Although many biochemistry instructors are gaining confidence in using…

  9. Uncovering Students' Incorrect Ideas about Foundational Concepts for Biochemistry

    ERIC Educational Resources Information Center

    Villafane, Sachel M.; Loertscher, Jennifer; Minderhout, Vicky; Lewis, Jennifer E.

    2011-01-01

    This paper presents preliminary data on how an assessment instrument with a unique structure can be used to identify common incorrect ideas from prior coursework at the beginning of a biochemistry course, and to determine whether these ideas have changed by the end of the course. The twenty-one multiple-choice items address seven different…

  10. Synthesis of Vitamin K Expoxide: An Undergraduate Biochemistry Experiment.

    ERIC Educational Resources Information Center

    Thierry-Palmer, M.

    1984-01-01

    Provides procedures for synthesizing and purifying a vitamin K metabolite (2,3-epoxide) to introduce many of the techniques used in lipid biochemistry. Includes typical results obtained as well as an optional experiment designed to test the purity of the epoxide obtained. (JM)

  11. Differentiating Biochemistry Course Laboratories Based on Student Experience

    ERIC Educational Resources Information Center

    Jakubowski, Henry V.

    2011-01-01

    Content and emphases in undergraduate biochemistry courses can be readily tailored to accommodate the standards of the department in which they are housed, as well as the backgrounds of the students in the courses. A more challenging issue is how to construct laboratory experiences for a class with both chemistry majors, who usually have little or…

  12. Biochemistry Instructors' Perceptions of Analogies and Their Classroom Use

    ERIC Educational Resources Information Center

    Orgill, MaryKay; Bussey, Thomas J.; Bodner, George M.

    2015-01-01

    Biochemistry education relies heavily on students' abilities to conceptualize abstract cellular and molecular processes, mechanisms, and components. From a constructivist standpoint, students build their understandings of these abstract processes by connecting, expanding, or revising their prior conceptions and experiences. As such, biochemistry…

  13. Assessment of Learning Gains in a Flipped Biochemistry Classroom

    ERIC Educational Resources Information Center

    Ojennus, Deanna Dahlke

    2016-01-01

    The flipped classroom has become an increasingly popular pedagogical approach to teaching and learning. In this study, learning gains were assessed in a flipped biochemistry course and compared to gains in a traditional lecture. Although measured learning gains were not significantly different between the two courses, student perception of…

  14. Ribose 5-Phosphate Isomerase Investigations for the Undergraduate Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Jewett, Kathy; Sandwick, Roger K.

    2011-01-01

    The enzyme ribose 5-phosphate isomerase (RpiA) has many features that make it attractive as a focal point of a semester-long, advanced biochemistry laboratory for undergraduate students. The protein can easily and inexpensively be isolated from spinach using traditional purification techniques. Characterization of RpiA enzyme activity can be…

  15. Positron Emission Tomography: Human Brain Function and Biochemistry.

    ERIC Educational Resources Information Center

    Phelps, Michael E.; Mazziotta, John C.

    1985-01-01

    Describes the method, present status, and application of positron emission tomography (PET), an analytical imaging technique for "in vivo" measurements of the anatomical distribution and rates of specific biochemical reactions. Measurements and image dynamic biochemistry link basic and clinical neurosciences with clinical findings suggesting…

  16. An "in Silico" DNA Cloning Experiment for the Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Elkins, Kelly M.

    2011-01-01

    This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced…

  17. A Problem-Based Learning Design for Teaching Biochemistry.

    ERIC Educational Resources Information Center

    Dods, Richard F.

    1996-01-01

    Describes the design of a biochemistry course that uses problem-based learning. Provides opportunities for students to question, dispute, confirm, and disconfirm their understanding of basic concepts. Emphasizes self-correction through dialogue. Topics covered include amino acids, metabolic pathways and inherited disease, proteins, enzymes and…

  18. Raising Environmental Awareness through Applied Biochemistry Laboratory Experiments

    ERIC Educational Resources Information Center

    Salman Ashraf, S.

    2013-01-01

    Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can provide solutions to such real-life issues. To this end, a newly developed biochemistry laboratory experiment…

  19. The Use of Multiple Tools for Teaching Medical Biochemistry

    ERIC Educational Resources Information Center

    Se, Alexandre B.; Passos, Renato M.; Ono, Andre H.; Hermes-Lima, Marcelo

    2008-01-01

    In this work, we describe the use of several strategies employing the philosophies of active learning and problem-based learning (PBL) that may be used to improve the teaching of metabolic biochemistry to medical and nutritional undergraduate students. The main activities are as follows: 1) a seminar/poster system in a mini-congress format (using…

  20. Concepts and Skills in the Biochemistry/Molecular Biology Lab

    ERIC Educational Resources Information Center

    Boyer, Rodney

    2003-01-01

    Most colleges and universities throughout the world now offer a Biochemistry/Molecular Biology (BMB) lab course that is designed for undergraduate students in the molecular life sciences, chemistry, and related fields. To best serve our students, we must introduce them to the most current concepts, skills, and methods available. Suggestions for…

  1. Computerized Examination: Application to the Clinical Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Bachmann, Kenneth A.

    1975-01-01

    Explains a computer exam format used with pharmacy students in a clinical biochemistry laboratory course. Student attitudes were assessed. Students felt that their final grade reflected their comprehension, exam pratical facilitated learning, exam format fostered retention and the questions were appropriate. (GS)

  2. Active Learning of Biochemistry Made Easy (for the Teacher)

    ERIC Educational Resources Information Center

    Bobich, Joseph A.

    2008-01-01

    This active learning pedagogical technique aims to improve students' learning in a two-semester, upper-division biochemistry course sequence in which the vast majority of students enrolled will continue on to medical or graduate schools. Instead of lecturing, the Instructor moves to the side of the room, thereby becoming "the guide on the side".…

  3. Osmotic Stressing, Membrane Leakage, and Fluorescence: An Introductory Biochemistry Demonstration

    ERIC Educational Resources Information Center

    Seu, Kalani J.

    2015-01-01

    A fluorescence demonstration is described that incorporates several fundamental aspects of an introductory biochemistry course. A variation of a known leakage assay is utilized to prepare vesicles containing a quenched fluorophore. The vesicles are exposed to several osmotic environments ranging from isotonic to hypotonic. The degree of vesicle…

  4. Raising environmental awareness through applied biochemistry laboratory experiments.

    PubMed

    Salman Ashraf, S

    2013-01-01

    Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can provide solutions to such real-life issues. To this end, a newly developed biochemistry laboratory experiment is described that guides students to learn about the applicability of peroxidase enzymes to degrade organic dyes (as model pollutants) in simulated waste water. In addition to showing how enzymes can potentially be used for waste water remediation, various factors than can affect enzyme-based reactions such as pH, temperature, concentration of substrates/enzymes, and denaturants can also be tested. This "applied biotechnology" experiment was successfully implemented in an undergraduate biochemistry laboratory course to enhance students' learning of environmental issues as well important biochemistry concepts. Student survey confirmed that this laboratory experiment was successful in achieving the objectives of raising environmental awareness in students and illustrating the usefulness of chemistry in solving real-life problems. This experiment can be easily adopted in an introductory biochemistry laboratory course and taught as an inquiry-guided exercise.

  5. Marine Biochemistry: A New Interdisciplinary Course for the Interim

    ERIC Educational Resources Information Center

    Goldberg, Arthur S.

    1976-01-01

    Discusses an undergraduate course which includes lectures, laboratory, and field trips and is designed for the interim winter semester. The course is interdisciplinary, involving a study of the biochemistry, pharmacology, and physiological significance of compounds from marine flora and fauna. (MLH)

  6. An Integrated Strategy for Teaching Biochemistry to Biotechnology Specialty Students

    ERIC Educational Resources Information Center

    Ouyang, Liming; Ou, Ling; Zhang, Yuanxing

    2007-01-01

    The faculty of biochemistry established an integrated teaching strategy for biotechnology specialty students, by intermeshing the case-study method, web-assistant teaching, and improved lecture format with a brief content and multimedia courseware. Teaching practice showed that the integrated teaching strategy could retain the best features of…

  7. Development of Student Writing in Biochemistry Using Calibrated Peer Review

    ERIC Educational Resources Information Center

    Hartberg, Yasha; Gunersel, Adalet Baris; Simspon, Nancy J.; Balester, Valerie

    2008-01-01

    This study investigating the effectiveness of Calibrated Peer Review (CPR )[TM] in a senior-level biochemistry class had three purposes: to (a) compare the CPR process for feedback with TA-generated feedback in improving students' ability to write scientific abstracts; (b) compare CPR results for males and females; and (c) observe whether CPR…

  8. Biochemistry in Undergraduate Health Courses: Structure and Organization

    ERIC Educational Resources Information Center

    Silva, Irani F.; Batista, Nildo A.

    2003-01-01

    This article describes the following aspects of teaching biochemistry in undergraduate health courses: objectives, number of hours, time in which the subject is studied, selection of content, teaching strategies, and evaluation methodologies used. Fifty-three courses distributed in 13 areas within the health field and offered by 12 institutions…

  9. Biochemistry Students' Ideas about Shape and Charge in Enzyme-Substrate Interactions

    ERIC Educational Resources Information Center

    Linenberger, Kimberly J.; Bretz, Stacey Lowery

    2014-01-01

    Biochemistry is a visual discipline that requires students to develop an understanding of numerous representations. However, there is very little known about what students actually understand about the representations that are used to communicate ideas in biochemistry. This study investigated biochemistry students' understanding of multiple…

  10. Maltose Biochemistry and Transport in Plant Leaves

    SciTech Connect

    Thomas D. Sharkey

    2010-01-28

    Final Technical Report for DOE grant DE-FG02-04ER15565 Maltose Biochemistry and Transport in Plant Leaves PI Thomas D. Sharkey University of Wisconsin-Madison Starch is a desirable plant product for both food and biofuel. Leaf starch is ideal for use in biofuels because it does not compete with grain starch, which is used for food. Starch is accumulated in plant leaves during the day and broken down at night. If we can manipulate leaf starch breakdown it may be possible to design a plant that provides both grain starch for food and leaf starch for biofuel. The pathway of leaf starch breakdown was not known when this work started. Preliminary evidence had shown that maltose was the primary product of leaf starch breakdown (Weise, Weber & Sharkey, 2004) and that it was metabolized by a disproportionating enzyme called amylomaltase but given the initials DPE2 (Lu & Sharkey, 2004). In this work we showed that only one form of maltose was metabolically active (Weise et al., 2005a) and that maltose was located in two different places when the amylomaltase was knocked out but only inside the chloroplast when the maltose transporter was knocked out (Lu et al., 2006a). This allowed us to estimate the energetics of maltose export and to show that maltose export is more efficient than glucose export (Weise et al., 2005b). We examined how daylength affected starch breakdown rate and found that starch breakdown rate could respond to changes in daylength within one day (Lu, Gehan & Sharkey, 2005). We also were able to show a second starch breakdown pathway by chloroplastic starch phosphorylase (Weise et al., 2006). Work to this point was summarized in a review (Lu & Sharkey, 2006). We were able to show that the amylomaltase in plants could substitute for the amylomaltase in bacteria (Lu et al., 2006b). In this paper we also showed the importance of a second enzyme called alpha-glucan phosphorylase in starch breakdown. Finally, we were able to determine the enzymatic mechanism of

  11. Playing with a double-edged sword: Analogies in biochemistry

    NASA Astrophysics Data System (ADS)

    Orgill, Marykay

    Analogy pervades our everyday reasoning. No situation we encounter is exactly like a situation we have encountered previously, and our ability to learn and survive in the world is based on our ability to find similarities between past and present situations and use the knowledge we have gained from past situations to manage current situations. Analogies can be powerful teaching tools because they can make new material intelligible to students by comparing it to material that is already familiar. It is clear, though, that not all analogies are good and that not all good analogies are useful to all students. In this study, I have used textbook analysis, classroom observations, student interviews and instructor interviews to determine the role that analogies play in biochemistry learning. Analogies are an important teaching technique in biochemistry classes, being used more often in both biochemistry classes and textbooks than they are in high school chemistry classes and textbooks. Most biochemistry students like, pay particular attention to, and remember the analogies their instructors provide; and they use these analogies to understand, visualize, and recall information from class. Even though students like and use analogies, they do not understand what analogies are or the mechanism by which they improve learning. For the students, analogies are simply any teaching technique that eases understanding, visualization, or recall. Instructors, on the other hand, have a good understanding of what analogies are and of how they should be presented in class; but they do not use analogies as effectively as they should. They do not plan, explain or identify the limitations of the analogies they use in class. However, regardless of how effectively instructors present analogies in class, this study indicates that, in general, analogies are useful in promoting understanding, visualization, recall, and motivation in biochemistry students at all levels. They would be even more

  12. Isotopic inferences of ancient biochemistries - Carbon, sulfur, hydrogen, and nitrogen

    NASA Technical Reports Server (NTRS)

    Schidlowski, M.; Hayes, J. M.; Kaplan, I. R.

    1983-01-01

    In processes of biological incorporation and subsequent biochemical processing sizable isotope effects occur as a result of both thermodynamic and kinetic fractionations which take place during metabolic and biosynthetic reactions. In this chapter a review is provided of earlier work and recent studies on isotope fractionations in the biogeochemical cycles of carbon, sulfur, hydrogen, and nitrogen. Attention is given to the biochemistry of carbon isotope fractionation, carbon isotope fractionation in extant plants and microorganisms, isotope fractionation in the terrestrial carbon cycle, the effects of diagenesis and metamorphism on the isotopic composition of sedimentary carbon, the isotopic composition of sedimentary carbon through time, implications of the sedimentary carbon isotope record, the biochemistry of sulfur isotope fractionation, pathways of the biogeochemical cycle of nitrogen, and the D/H ratio in naturally occurring materials.

  13. How to Search for Life by the Detection of Biochemistry

    NASA Astrophysics Data System (ADS)

    McKay, C. P.; Davila, A.; Sun, H. J.

    2013-12-01

    We consider how to search for life by the detection of biochemistry in three relevance case: 1) in samples returned to Earth, 2) In situ in the organic rich plume of Enceladus, and 3) On Mars, following the discovery of organics. A search for organic biomarkers can address several questions including: 1) Evidence for present or past life, 2) Evidence for a second genesis of life, 3) Hazard assessment for human explorers and sample return and 4) Detection of bioload from Earth. Some useful analogs for the search for organic biomarkers on other worlds include 1) Ancient Earth sediment record, an example of a poorly preserved ancient biochemistry, 2) Modern environments including anoxic Antarctic sediments 3)Extreme cold desert surfaces in the High Antarctic Dry Valleys 4) Extremely dry soils such as the Atacama Desert 5) Evaporites. Sample preparation is a key issue, often unappreciated in past. Illustrated by the interference of perchlorate with organic detection on Viking and SAM.

  14. Assessment of learning gains in a flipped biochemistry classroom.

    PubMed

    Ojennus, Deanna Dahlke

    2016-01-01

    The flipped classroom has become an increasingly popular pedagogical approach to teaching and learning. In this study, learning gains were assessed in a flipped biochemistry course and compared to gains in a traditional lecture. Although measured learning gains were not significantly different between the two courses, student perception of learning gains did differ and indicates a higher level of satisfaction with the flipped lecture format.

  15. Enhanced podcasts for teaching biochemistry to veterinary students.

    PubMed

    Gough, Kevin C

    2011-01-01

    The teaching of biochemistry within medical disciplines presents certain challenges; firstly to relay a large body of complex facts and abstract concepts, and secondly to motivate students that this relatively difficult topic is worth their time to study. Here, nutrient biochemistry was taught within a multidisciplinary module as part of an undergraduate veterinary curriculum. The teaching approach was initially focussed on a mixture of didactic lectures and student-centred activities such as directed group/self learning. In subsequent years the core didactic lectures were replaced with enhanced podcasts covering the same material, along with the introduction of student presentations delivered within groups with both peer and facilitator assessment. These changes were accompanied by an increase in the time dedicated to this topic to allow sufficient time for students to work through podcasts and prepare presentations. The combination of these changes resulted in significant improvements in student performance within an in-course biochemistry long essay. These changes in the teaching approach, and particularly the introduction of extensive podcasts, was well received by students who perceived the process of going through the podcasts as time consuming but allowing them flexibility in both the pace that they studied this topic as well as the location and times that they studied it.

  16. The concept lens diagram: a new mechanism for presenting biochemistry content in terms of "big ideas".

    PubMed

    Rowland, Susan L; Smith, Christopher A; Gillam, Elizabeth M A; Wright, Tony

    2011-07-01

    A strong, recent movement in tertiary education is the development of conceptual, or "big idea" teaching. The emphasis in course design is now on promoting key understandings, core competencies, and an understanding of connections between different fields. In biochemistry teaching, this radical shift from the content-based tradition is being driven by the "omics" information explosion; we can no longer teach all the information we have available. Biochemistry is a core, enabling discipline for much of modern scientific research, and biochemistry teaching is in urgent need of a method for delivery of conceptual frameworks. In this project, we aimed to define the key concepts in biochemistry. We find that the key concepts we defined map well onto the core science concepts recommended by the Vision and Change project. We developed a new method to present biochemistry through the lenses of these concepts. This new method challenged the way we thought about biochemistry as teachers. It also stimulated the majority of the students to think more deeply about biochemistry and to make links between biochemistry and material in other courses. This method is applicable to the full spectrum of content usually taught in biochemistry.

  17. [Biochemistry, molecular mechanism of action and biological effects of endotoxin].

    PubMed

    Burgmann, H; Breyer, S

    1995-01-01

    This review is a brief attempt at providing an overview of a subject of enormous complexity-endotoxins and the mediators associated with its biological effects. More specifically it deals with biochemistry and biology of endotoxin, detection of endotoxin with the Limulus amebocyte lysate test, the molecular mechanisms and biological effects, and in the last part with future aspects of therapeutical strategies. It seems certain that the subject will become even more complex and possibly controversial as scientific knowledge further involves. However, because of the high mortality rate of patients suffering from gram-negative sepsis all efforts have to be made to find effective therapeutical strategies.

  18. [Frequency and antimicrobial resistance of Acinetobacter species in a university hospital of Buenos Aires City].

    PubMed

    Rodríguez, Carlos Hernán; Nastro, Marcela; Dabos, Laura; Vay, Carlos; Famiglietti, Angela

    2014-01-01

    Two-hundred Acinetobacter isolates belonging to 200 patients admitted to Hospital de Clínicas José de San Martín during the period March 2013-June 2014 were analyzed. The identification was performed by mass spectrometry and was confirmed by molecular methods. Susceptibility to antimicrobials was studied by the Vitek-2 system. A 94% correlation of both identification methods was found. Multidrug resistant Acinetobacter baumannii was the predominant genomic species (92.6%) in hospital-acquired infections, whereas Acinetobacter pitti and Acinetobacter nosocomialis accounted for 3.5% and 0.5% of the isolates recovered, respectively. In community-acquired infections a major predominance of the different genomic species was observed. Acinetobacter johnsonii and A. baumannii are the most frequent species, accounting for 45.9% of the isolates recovered. Resistance to carbapenems and minocycline was only observed in A. baumannii. Mass spectrophotometry was an effective tool for the identification of the different genomic species.

  19. A new species of Tullbergia (Collembola, Tullbergiidae) from Buenos Aires, Argentina

    PubMed Central

    Palacios-Vargas, José G.; Martínez, Ana E. Salazar

    2014-01-01

    Abstract A new species of Tullbergia from Argentina is described and illustrated; it is differentiated from Tullbergia paranensis by the number of vesicles of postantennal organ, pseudocelli shape and its formulae and the number of dorsal sensilla on Ant. IV. In addition a key for the identification of the members of the family from Argentina is included. PMID:25061344

  20. [Poverty, child malnutrition and morbidity in urban area families from Gran La Plata, Buenos Aires].

    PubMed

    Ortale, S; Rodrigo, M A

    1998-06-01

    A comparative study was carried out over two samples of 50 families each. Both groups belong to the low income social level from Gran La Plata. One of the groups was made up of families in which at least one child suffer malnutrition. The other group of families did not present children under malnutrition conditions. Differences sociodemographic, economic and environmental factors are identified. At the same time, a strong link between primary malnutrition and an increase in children morbility and mortality was found. The results obtained demonstrate that the lack of programmes that these cases deserve, have a high social and economic cost, as malnutrition treatment demand a higher budget than its prevention.

  1. Ecotoxicity in the Reconquista River, province of Buenos Aires, Argentina: a preliminary study.

    PubMed Central

    Herkovits, J; Perez-Coll, C S; Herkovits, F D

    1996-01-01

    The Reconquista River in Argentina is considered a "supercritical" river basin due to environmental degradation. Within its valley of 1.547 km2, there are more than 3 million inhabitants and 12,000 industries. Using early-life-stage toxicity tests with Bufo arenarum embryos (the most sensitive of three native species), we determined the water quality at six sampling stations of the river valley and expressed the results as acute and chronic toxicity units. Along most of the river, the toxicity was higher than the allowable level of whole industrial effluent toxicity recommended by U.S. EPA. In a tributary stream, Arroyo Moron, the water was about 10 times more toxic than the criteria maximum concentration (CMC) recommended by U.S. EPA for industrial effluents. Similar degradation of the water quality was found taking as a reference value an upstream sampling station. In all places where the water quality was worse than the CMC, no macroorganisms were found, and in one of these places, a large number of dead fishes was observed. Our study points out that amphibian early-life-stage toxicity tests could be appropriate for assessing water contamination and water quality, which is essential for diagnosis, protection of environmental services, monitoring, and restoration purposes. Images Figure 1. Figure 2. Figure 3. Figure 4. Figure 5. Figure 6. PMID:8820587

  2. [Clostridium difficile diarrhea: frequency of detection in a medical center in Buenos Aires, Argentina].

    PubMed

    Fernandez Canigia, L; Nazar, J; Arce, M; Dadamio, J; Smayevsky, J; Bianchini, H

    2001-01-01

    Clostridium difficile has been recognized as the most important enteric pathogen of nosocomial antibiotic-associated diarrhea (CDAD) in adults from industrialized countries. The importance of C. difficile as a cause of diarrhea in ambulatory patients appears underestimated or under-recognized. Since the 1980's, outbreaks of CDAD have been increasingly reported, but there are few data available in Argentina. We developed a retrospective study to provide some information about CDAD in our country. From July 1998 to November 1999, a total of 245 fecal specimens from hospitalized and some ambulatory patients were tested in order to confirm the diagnosis of CDAD. C. difficile cytotoxin (toxin B) was identified by detecting its cytopathic effect on monolayers of McCoy culture cells. For culture and isolation of C. difficile, stool samples were prepared by ethanol shock prior to plating onto a selective medium which contained blood, cefoxitin and fructose. Of the 245 samples, 14 (5.8%) were identified as positive by the cell cytotoxicity assay. Using the criteria of isolation of cytotoxigenic C. difficile positivity increased to 6.5% (16 samples). Thirteen of the positive results were from hospitalized patients (81.3%) and 3 (18.7%) from outpatients. The mean age of inpatients was 72.9 years (ranging from 47 to 88). All patients had received 2 or more antimicrobial agents (most of them beta-lactams) 2 months before the appearance of diarrhea. There was one patient who had received only chemotherapy. The prevalence of CDAD in this study was less than in others previously reported. This difference may be due to the fact that not all general practitioners include testing for C. difficile when the patient with diarrhea had previously received antibiotics. More educational programs should be directed to all physicians, concerning the role of C. difficile as an important enteric pathogen in patients who have undergone treatment with antimicrobial or chemotherapeutic agents.

  3. Comparing International Student Friendship Networks in Buenos Aires: Direct Enrollment Programs vs. Study Abroad Centers

    ERIC Educational Resources Information Center

    Hendrickson, Blake

    2016-01-01

    In March of 2011 Unite States president Barack Obama announced the "100,000 strong in the Americas" program during his visit to Chile (U.S. State Department, 2011). The initiative's goal is to reach 100,000 U.S. students studying in Latin America and 100,000 Latin American students studying in the United States. This interchange of…

  4. Ecotoxicity in the Reconquista River, province of Buenos Aires, Argentina: a preliminary study.

    PubMed

    Herkovits, J; Perez-Coll, C S; Herkovits, F D

    1996-02-01

    The Reconquista River in Argentina is considered a "supercritical" river basin due to environmental degradation. Within its valley of 1.547 km2, there are more than 3 million inhabitants and 12,000 industries. Using early-life-stage toxicity tests with Bufo arenarum embryos (the most sensitive of three native species), we determined the water quality at six sampling stations of the river valley and expressed the results as acute and chronic toxicity units. Along most of the river, the toxicity was higher than the allowable level of whole industrial effluent toxicity recommended by U.S. EPA. In a tributary stream, Arroyo Moron, the water was about 10 times more toxic than the criteria maximum concentration (CMC) recommended by U.S. EPA for industrial effluents. Similar degradation of the water quality was found taking as a reference value an upstream sampling station. In all places where the water quality was worse than the CMC, no macroorganisms were found, and in one of these places, a large number of dead fishes was observed. Our study points out that amphibian early-life-stage toxicity tests could be appropriate for assessing water contamination and water quality, which is essential for diagnosis, protection of environmental services, monitoring, and restoration purposes. PMID:8820587

  5. Microstructural analysis and P T conditions of the Azul megashear zone, Tandilia, Buenos Aires province, Argentina

    NASA Astrophysics Data System (ADS)

    Frisicale, M. C.; Martinez, F. J.; Dimieri, L. V.; Dristas, J. A.

    2005-09-01

    A microstructural analysis was carried out on mylonitic rocks of the Azul megashear zone (AMSZ), Tandilia, which were formed in a range of metamorphic conditions from lower greenschist to amphibolite facies. Tailed porphyroclasts are common and mostly symmetric. Scarce asymmetric rotated porphyroclasts show both sinistral and dextral senses of shear. In sections parallel to the mylonitic foliation, porphyroclasts are round. The AMSZ is probably related to the late Transamazonian orogenic cycle and may be due to NNE-SSW-directed convergence. In weakly deformed protolith and protomylonites, quartz deforms by dynamic recrystallization, mainly subgrain rotation in dislocation creep Regime 2. K-feldspar porphyroclasts and plagioclase show scarce fracturation and deform by dynamic recrystallization along grain boundaries. Quartz microstructures in mylonites indicate predominantly Regime 3 grain boundary migration recrystallization. Feldspar structures indicate recrystallization through the nucleation and growth of new grains at grain boundaries. The temperatures of deformation from mineral assemblages in the CNKFMASH system in four bulk compositions are in the range of 400-450 °C, and the pressures are more than 6 kb.

  6. Autoantibodies in adult patients with idiopathic inflammatory myopathies in Buenos Aires.

    PubMed

    Gómez, Graciela N; Gargiulo, María De Los Ángeles; Pérez, Nicolás; Collado, María Victoria; Suárez, Lorena V; Khoury, Marina; Sarano, Judith F

    2016-01-01

    The idiopathic inflammatory myopathies(IIM) are a heterogeneous group of diseases of the skeletal muscle. On the basis of clinical, serologic and histological differences, they are classified in dermatomyositis (DM), polymyositis (PM), inclusion body myositis and immunomediated necrotizing myopathy. Autoantibodies directed against nuclear and cytoplasmic antigens are present with variable frequencies among studies. Myositis-specific antibodies (MSAs) are useful in IIM because they contribute to the diagnosis, help to identify different clinical subsets, and have prognostic value. This study aimed to explore the frequency of autoantibodies, especially MSAs, and their relationship with clinical features in adult patients with DM, PM and overlap syndrome. Medical records were reviewed. Myositis-associated antibodies (non-specific) and MSAs (anti Jo-1, PL-7, PL-12, Mi-2 and SRP) were measured using commercial kits. Twelve patients had MSAs, an overall frequency similar to those of international series, but PL-12 and Mi-2 were more frequent than Jo-1, which is the most frequently observed elsewhere. All five patients with Mi-2 had classical DM with a favorable response to treatment. Interstitial pneumonia (n: 4) and/or treatment-refractory disease (n: 3) were found in the presence of anti-PL-12, alone or associated with anti-SRP and/or Jo-1. In conclusion, the coexistence of AEM, a rare finding, was found in three patients. The presence of MSAs aided to the diagnosis of IIM, in particular in those patients without available or conclusive biopsy results.

  7. Land use effect on invertebrate assemblages in Pampasic streams (Buenos Aires, Argentina).

    PubMed

    Solis, Marina; Mugni, Hernán; Hunt, Lisa; Marrochi, Natalia; Fanelli, Silvia; Bonetto, Carlos

    2016-09-01

    Agriculture and livestock may contribute to water quality degradation in adjacent waterbodies and produce changes in the resident invertebrate composition. The objective of the present study was to assess land use effects on the stream invertebrate assemblages in rural areas of the Argentine Pampa. The four sampling events were performed at six sites in four streams of the Pampa plain; two streams were sampled inside a biosphere reserve, and another one was surrounded by extensive livestock fields. The fourth stream was sampled at three sites; the upstream site was adjacent to agricultural plots, the following site was adjacent to an intensive livestock plot and the downstream site was adjacent to extensive breeding cattle plots. Higher pesticide concentrations were found at the site adjacent to agricultural plots and higher nutrient concentrations at the sites adjacent to agricultural and intensive breeding cattle plots. The invertebrate fauna were also different at these sites. Multivariate analysis showed a relationship between nutrient concentrations and taxonomic composition. Amphipoda (Hyalella curvispina) was the dominant group in the reserve and extensive breeding cattle sites, but was not present in the agricultural site. Also, Chironomidae were absent from the agricultural site while present at other sites. Gasteropoda (Biomphalaria peregrina), Zygoptera, and Hirudinea were dominant at the most impacted agricultural and intensive breeding cattle sites. PMID:27581006

  8. Autoantibodies in adult patients with idiopathic inflammatory myopathies in Buenos Aires.

    PubMed

    Gómez, Graciela N; Gargiulo, María De Los Ángeles; Pérez, Nicolás; Collado, María Victoria; Suárez, Lorena V; Khoury, Marina; Sarano, Judith F

    2016-01-01

    The idiopathic inflammatory myopathies(IIM) are a heterogeneous group of diseases of the skeletal muscle. On the basis of clinical, serologic and histological differences, they are classified in dermatomyositis (DM), polymyositis (PM), inclusion body myositis and immunomediated necrotizing myopathy. Autoantibodies directed against nuclear and cytoplasmic antigens are present with variable frequencies among studies. Myositis-specific antibodies (MSAs) are useful in IIM because they contribute to the diagnosis, help to identify different clinical subsets, and have prognostic value. This study aimed to explore the frequency of autoantibodies, especially MSAs, and their relationship with clinical features in adult patients with DM, PM and overlap syndrome. Medical records were reviewed. Myositis-associated antibodies (non-specific) and MSAs (anti Jo-1, PL-7, PL-12, Mi-2 and SRP) were measured using commercial kits. Twelve patients had MSAs, an overall frequency similar to those of international series, but PL-12 and Mi-2 were more frequent than Jo-1, which is the most frequently observed elsewhere. All five patients with Mi-2 had classical DM with a favorable response to treatment. Interstitial pneumonia (n: 4) and/or treatment-refractory disease (n: 3) were found in the presence of anti-PL-12, alone or associated with anti-SRP and/or Jo-1. In conclusion, the coexistence of AEM, a rare finding, was found in three patients. The presence of MSAs aided to the diagnosis of IIM, in particular in those patients without available or conclusive biopsy results. PMID:27295700

  9. [Amputations. A five-year epidemiological study in Buenos Aires City].

    PubMed

    Mendelevich, Alejandro; Kramer, Marcia; Maiarú, Mariano; Módica, Mariela; Ostolaza, Marco; Peralta, Federico

    2015-01-01

    An amputation is the removal of a limb by surgery or accident. The aim of this paper was to carry out an epidemiological analysis on patients with amputations treated at the Instituto de Rehabilitación Psicofísica between 2009 and 2013. This is a retrospective, observational and cross-sectional study. The analyzed data included a total of 262 patients: 180 men (68.7%) and 82 women (31.3%). Our population mainly included subjects with only one amputated lower limb (83.6%), of vascular etiology (mostly diabetics) and with an average age of 63.5 years. The second sample group comprised traumatic amputees (29.8%), with an average age of 37 years and with a higher frequency of phantom pain. The characteristics of these groups can make them to behave differently during rehabilitation.

  10. Ecotoxicity in the Reconquista River, province of Buenos Aires, Argentina: a preliminary study.

    PubMed

    Herkovits, J; Perez-Coll, C S; Herkovits, F D

    1996-02-01

    The Reconquista River in Argentina is considered a "supercritical" river basin due to environmental degradation. Within its valley of 1.547 km2, there are more than 3 million inhabitants and 12,000 industries. Using early-life-stage toxicity tests with Bufo arenarum embryos (the most sensitive of three native species), we determined the water quality at six sampling stations of the river valley and expressed the results as acute and chronic toxicity units. Along most of the river, the toxicity was higher than the allowable level of whole industrial effluent toxicity recommended by U.S. EPA. In a tributary stream, Arroyo Moron, the water was about 10 times more toxic than the criteria maximum concentration (CMC) recommended by U.S. EPA for industrial effluents. Similar degradation of the water quality was found taking as a reference value an upstream sampling station. In all places where the water quality was worse than the CMC, no macroorganisms were found, and in one of these places, a large number of dead fishes was observed. Our study points out that amphibian early-life-stage toxicity tests could be appropriate for assessing water contamination and water quality, which is essential for diagnosis, protection of environmental services, monitoring, and restoration purposes.

  11. School Autonomy in the Province of Buenos Aires, Argentina: Evidence from Two School Districts

    ERIC Educational Resources Information Center

    Astiz, M. Fernanda

    2006-01-01

    This article provides empirical evidence of policy adoption, outcomes and consequences of decentralization and school autonomy initiatives enacted in Argentina during the 1990s. The study examines what school autonomy meant in Argentina and how it was adopted at the provincial and school levels. Using qualitative data on school districts of the…

  12. Ethanol Metabolism and the Transition from Organic Chemistry to Biochemistry

    NASA Astrophysics Data System (ADS)

    Feinman, Richard D.

    2001-09-01

    To ease the transition from organic chemistry at the beginning of a biochemistry course or at the beginning of the metabolism section of the organic course, an early presentation of the oxidation of ethanol is proposed. Alcohol dehydrogenase and aldehyde dehydrogenase reactions can smooth the introduction to biochemistry, since they involve three of the simplest compounds: ethanol, acetaldehyde, and acetic acid. Using these reactions as a model encourages the study of metabolic pathways by a systematic approach rather than by rote memorization. Reactions that can be presented as variations on a theme include methanol poisoning, the polyol reaction, and, most important, the sequence glycerol-3-phosphate to glyceraldehyde-3-phosphate to 3-phosphoglyceric acid. This last sequence integrates lipid and carbohydrate metabolism and, by comparison with the model reaction, brings out the principles of substrate-level phosphorylation. The method has evoked favorable verbal feedback from students and, in addition to medical and graduate courses, has been successfully used in the biochemical section of an undergraduate organic course.

  13. A national comparison of biochemistry and molecular biology capstone experiences.

    PubMed

    Aguanno, Ann; Mertz, Pamela; Martin, Debra; Bell, Ellis

    2015-01-01

    Recognizing the increasingly integrative nature of the molecular life sciences, the American Society for Biochemistry and Molecular Biology (ASBMB) recommends that Biochemistry and Molecular Biology (BMB) programs develop curricula based on concepts, content, topics, and expected student outcomes, rather than courses. To that end, ASBMB conducted a series of regional workshops to build a BMB Concept Inventory containing validated assessment tools, based on foundational and discipline-specific knowledge and essential skills, for the community to use. A culminating activity, which integrates the educational experience, is often part of undergraduate molecular life science programs. These "capstone" experiences are commonly defined as an attempt to measure student ability to synthesize and integrate acquired knowledge. However, the format, implementation, and approach to outcome assessment of these experiences are quite varied across the nation. Here we report the results of a nation-wide survey on BMB capstone experiences and discuss this in the context of published reports about capstones and the findings of the workshops driving the development of the BMB Concept Inventory. Both the survey results and the published reports reveal that, although capstone practices do vary, certain formats for the experience are used more frequently and similarities in learning objectives were identified. The use of rubrics to measure student learning is also regularly reported, but details about these assessment instruments are sparse in the literature and were not a focus of our survey. Finally, we outline commonalities in the current practice of capstones and suggest the next steps needed to elucidate best practices.

  14. The Use of Case Studies in an Undergraduate Biochemistry Course

    NASA Astrophysics Data System (ADS)

    Cornely, Kathleen

    1998-04-01

    Most college biochemistry courses are taught in a format in which the professor lectures and the student memorizes. Although this is the best method for conveying large amounts of material, it puts the student in the position of passive learner. The lecture-based format has not been abandoned, but has been supplemented with case study projects assigned to the students upon completion of the intermediary metabolism unit. The case study assignment is modeled on similar exercises carried out in medical school biochemistry courses in the US and around the world. A description of the assignment follows: a group of 4-5 students is given a case study which gives the medical history of a patient with an inherited metabolic disease. The group is asked to provide biochemical explanations for the patient's symptoms and to suggest an effective course of treatment. The evaluation consists of a short paper that the students write as a group. The assignment provides the opportunity for small group interaction within a larger class and emphasizes cooperative-collaborative learning. Students learn by researching the topic on their own and debating it in small group discussions, and in so doing, gain a sense of confidence in themselves and the material they have learned over the course of the semester. Solving a "real-life" problem helps develop analytical and higher-order thinking skills and allows the students to see how biochemical concepts they have learned apply to a clinical situation.

  15. Incorporation of Ethical and Societal Issues in Biochemistry into a Senior Seminar Course

    ERIC Educational Resources Information Center

    Caspers, Mary Lou; Roberts-Kirchhoff, Elizabeth S.

    2003-01-01

    In their senior year, biochemistry majors at the University of Detroit Mercy take a senior seminar course entitled "Recent Advances in Biochemistry Related to Societal Issues." Students read papers selected from the current literature and take turns presenting these papers to the class. Papers are grouped into units dealing with molecular biology,…

  16. A Biochemistry of Human Disease Course for Undergraduate and Graduate Students.

    ERIC Educational Resources Information Center

    Glew, Robert H.; VanderJagt, David L.

    2001-01-01

    Describes the experiences of a medical school faculty who have been offering for more than 10 years a two-course series in the biochemistry of human disease to undergraduate students majoring in biochemistry, biology, or chemistry. Recommends the teaching of specialized, advanced courses to undergraduate, pre-professional students. (DDR)

  17. Biochemistry Instructors' Views toward Developing and Assessing Visual Literacy in Their Courses

    ERIC Educational Resources Information Center

    Linenberger, Kimberly J.; Holme, Thomas A.

    2015-01-01

    Biochemistry instructors are inundated with various representations from which to choose to depict biochemical phenomena. Because of the immense amount of visual know-how needed to be an expert biochemist in the 21st century, there have been calls for instructors to develop biochemistry students' visual literacy. However, visual literacy has…

  18. Biochemistry Students' Ideas about How an Enzyme Interacts with a Substrate

    ERIC Educational Resources Information Center

    Linenberger, Kimberly J.; Bretz, Stacey Lowery

    2015-01-01

    Enzyme-substrate interactions are a fundamental concept of biochemistry that is built upon throughout multiple biochemistry courses. Central to understanding enzyme-substrate interactions is specific knowledge of exactly how an enzyme and substrate interact. Within this narrower topic, students must understand the various binding sites on an…

  19. Case Study of How Turkish University Students Improve Their Biochemistry Achievement

    ERIC Educational Resources Information Center

    Sadi, Özlem

    2013-01-01

    Biochemistry courses have an important place as a common subject in faculties of medicine, food engineering, biology and chemistry. MSLQ, Metacognitive Awareness Inventory and Learning Approach Questionnaire were used. The study also involves repeated observations of the same instructor in a biochemistry class over eight weeks to describe…

  20. An Analysis of the Effectiveness of Analogy Use in College-Level Biochemistry Textbooks

    ERIC Educational Resources Information Center

    Orgill, MaryKay; Bodner, George M.

    2006-01-01

    Science instructors and textbook authors often use analogies to help their students use information they already understand to develop an understanding of new concepts. This study reports the results of an analysis of the use of analogies in eight biochemistry textbooks, which included textbooks written for one-semester survey biochemistry courses…

  1. Combining Content and Elements of Communication into an Upper-Level Biochemistry Course

    ERIC Educational Resources Information Center

    Whittington, Carli P.; Pellock, Samuel J.; Cunningham, Rebecca L.; Cox, James R.

    2014-01-01

    This report describes how a science communication module was incorporated into an advanced biochemistry course. Elements of communication were taught synergistically with biochemistry content in this course in an effort to expose students to a variety of effective oral communication strategies. Students were trained to use these established…

  2. Teaching Arrangements of Carbohydrate Metabolism in Biochemistry Curriculum in Peking University Health Science Center

    ERIC Educational Resources Information Center

    Chen, Hao; Ni, Ju-Hua

    2013-01-01

    Biochemistry occupies a unique place in the medical school curricula, but the teaching of biochemistry presents certain challenges. One of these challenges is facilitating students' interest in and mastery of metabolism. The many pathways and modes of regulation can be overwhelming for students to learn and difficult for professors to teach…

  3. Teaching Structure: Student Use of Software Tools for Understanding Macromolecular Structure in an Undergraduate Biochemistry Course

    ERIC Educational Resources Information Center

    Jaswal, Sheila S.; O'Hara, Patricia B.; Williamson, Patrick L.; Springer, Amy L.

    2013-01-01

    Because understanding the structure of biological macromolecules is critical to understanding their function, students of biochemistry should become familiar not only with viewing, but also with generating and manipulating structural representations. We report a strategy from a one-semester undergraduate biochemistry course to integrate use of…

  4. Suitable Class Experiments in Biochemistry for High-school Chemistry and Biology Courses.

    ERIC Educational Resources Information Center

    Myers, A.

    1987-01-01

    Illustrates the scope of experimental investigations for biochemistry education in high school biology and chemistry courses. Gives a brief overview of biochemistry experiments with proteins, enzymes, carbohydrates, lipids, nucleic acids, vitamins, metabolism, electron transport, and photosynthesis including materials, procedures, and outcomes.…

  5. An Undergraduate Biochemistry Laboratory Course with an Emphasis on a Research Experience

    ERIC Educational Resources Information Center

    Caspers, Mary Lou; Roberts-Kirchhoff, Elizabeth S.

    2003-01-01

    In their junior or senior year, biochemistry majors at the University of Detroit Mercy are required to take a two-credit biochemistry laboratory course. Five years ago, the format of this course was changed from structured experiments to a more project-based approach. Several structured experiments were included at the beginning of the course…

  6. Reactivity II: A Second Foundation-Level Course in Integrated Organic, Inorganic, and Biochemistry

    ERIC Educational Resources Information Center

    Schaller, Chris P.; Graham, Kate J.; McIntee, Edward J.; Jones, T. Nicholas; Johnson, Brian J.

    2016-01-01

    A foundation-level course is described that integrates material related to reactivity in organic, inorganic, and biochemistry. Designed for second-year students, the course serves majors in chemistry, biochemistry, and biology, as well as prehealth-professions students. Building on an earlier course that developed concepts of nucleophiles and…

  7. A Curriculum Skills Matrix for Development and Assessment of Undergraduate Biochemistry and Molecular Biology Laboratory Programs

    ERIC Educational Resources Information Center

    Caldwell, Benjamin; Rohlman, Christopher; Benore-Parsons, Marilee

    2004-01-01

    We have designed a skills matrix to be used for developing and assessing undergraduate biochemistry and molecular biology laboratory curricula. We prepared the skills matrix for the Project Kaleidoscope Summer Institute workshop in Snowbird, Utah (July 2001) to help current and developing undergraduate biochemistry and molecular biology program…

  8. Probing Changes in the Conformation of tRNA[superscript Phe]: An Integrated Biochemistry Laboratory Course

    ERIC Educational Resources Information Center

    Kirk, Sarah R.; Silverstein, Todd P.; McFarlane Holman, Karen L.; Taylor, Buck L. H.

    2008-01-01

    We have designed a new guided-inquiry laboratory for an advanced biochemistry course. This integrated laboratory focuses on the biomolecule tRNA[superscript Phe] and combines elements of bioorganic and bioinorganic chemistry with biochemistry. Throughout the semester students work together to study tRNA[superscript Phe] structure and ligand…

  9. Glycobiology, How to Sugar-Coat an Undergraduate Advanced Biochemistry Laboratory

    ERIC Educational Resources Information Center

    McReynolds, Katherine D.

    2006-01-01

    A second semester biochemistry laboratory has been implemented as an independent projects course at California State University, Sacramento since 1999. To incorporate aspects of carbohydrate biochemistry, or glycobiology, into our curriculum, projects in lectin isolation and purification were undertaken over the course of two semesters. Through…

  10. The Use of Contextual Learning to Teach Biochemistry to Dietetic Students

    ERIC Educational Resources Information Center

    Macaulay, J. O.; Van Damme, M. -P.; Walker, K. Z.

    2009-01-01

    This article describes the use of contextualized and "blended" learning to teach biochemistry to dietetic students during the second year of their professional training in a 4-year undergraduate degree (Bachelor of Nutrition and Dietetics). Contextualized content was used to engage students and motivate them to learn biochemistry, which many…

  11. What Are the Appropriate Curriculum Contents for Biochemistry Courses in Veterinary Medicine?

    ERIC Educational Resources Information Center

    Correia, A. A. D.; Correia, J. H. R. D.

    1995-01-01

    Presents an overview of the important items that the author's suggest should be included in a biochemistry course given to students in veterinary medicine. Presents a broad range of specific topics in biochemistry and strategies for covering as many topics as possible in one course. (LZ)

  12. Mammalian opiate alkaloid synthesis: lessons derived from plant biochemistry.

    PubMed

    Meijerink, W J; Molina, P E; Abumrad, N N

    1999-09-01

    The presence of opiate receptors in mammalian tissues has stimulated the search for endogenous ligands to these receptors and has led to the discovery and characterization of endogenous opioid peptides. However, recent studies have provided evidence for the presence of opiate alkaloids in mammalian tissues and for their endogenous synthesis. The study of their origin and synthetic pathway has been significantly influenced by the early classical biochemical studies performed in plants. This review is a historical account of the use and abuse of opiates, the elucidation of morphine's synthetic pathway in the poppy plant, and the subsequent characterization of its presence in mammalian tissues. Clearly, our understanding of its synthetic pathway and regulation is a reflection of observations originally made in plant biochemistry.

  13. A focused assignment encouraging deep reading in undergraduate biochemistry.

    PubMed

    Spiegelberg, Bryan D

    2014-01-01

    Encouraging undergraduate students to access, read, and analyze current primary literature can positively impact learning, especially in advanced courses. The incorporation of literature into coursework typically involves reading and responding to full research reports. Such exercises have clear value as students make connections between experiments and are able to probe and critique scientific logic. The exclusive use of full papers, though, may reinforce certain students' tendencies to rely on textual clues rather than a critical analysis of the actual data presented. I propose that structured activities requiring students to focus on individual parts of research papers, even on a single figure, are beneficial in a literature-centered advanced undergraduate course, because they promote the deep reading that is critical to scientific discourse. In addition, I describe how one such focused assignment boosted learning and was well received by students in a second-semester biochemistry course.

  14. Hallmarks of a new era in mitochondrial biochemistry

    PubMed Central

    Pagliarini, David J.; Rutter, Jared

    2013-01-01

    Stemming from the pioneering studies of bioenergetics in the 1950s, 1960s, and 1970s, mitochondria have become ingrained in the collective psyche of scientists as the “powerhouses” of the cell. While this remains a worthy moniker, more recent efforts have revealed that these organelles are home to a vast array of metabolic and signaling processes and possess a proteomic landscape that is both highly varied and largely uncharted. As mitochondrial dysfunction is increasingly being implicated in a spectrum of human diseases, it is imperative that we construct a more complete framework of these organelles by systematically defining the functions of their component parts. Powerful new approaches in biochemistry and systems biology are helping to fill in the gaps. PMID:24352419

  15. Whither life? Conjectures on the future evolution of biochemistry.

    PubMed

    Brewster, Jodi L; Finn, Thomas J; Ramirez, Miguel A; Patrick, Wayne M

    2016-08-01

    Life has existed on the Earth for approximately four billion years. The sheer depth of evolutionary time, and the diversity of extant species, makes it tempting to assume that all the key biochemical innovations underpinning life have already happened. But we are only a little over halfway through the trajectory of life on our planet. In this Opinion piece, we argue: (i) that sufficient time remains for the evolution of new processes at the heart of metabolic biochemistry and (ii) that synthetic biology is providing predictive insights into the nature of these innovations. By way of example, we focus on engineered solutions to existing inefficiencies in energy generation, and on the complex, synthetic regulatory circuits that are currently being implemented. PMID:27555646

  16. Whither life? Conjectures on the future evolution of biochemistry

    PubMed Central

    Brewster, Jodi L.; Finn, Thomas J.; Ramirez, Miguel A.

    2016-01-01

    Life has existed on the Earth for approximately four billion years. The sheer depth of evolutionary time, and the diversity of extant species, makes it tempting to assume that all the key biochemical innovations underpinning life have already happened. But we are only a little over halfway through the trajectory of life on our planet. In this Opinion piece, we argue: (i) that sufficient time remains for the evolution of new processes at the heart of metabolic biochemistry and (ii) that synthetic biology is providing predictive insights into the nature of these innovations. By way of example, we focus on engineered solutions to existing inefficiencies in energy generation, and on the complex, synthetic regulatory circuits that are currently being implemented. PMID:27555646

  17. Myoglobin structure and function: A multiweek biochemistry laboratory project.

    PubMed

    Silverstein, Todd P; Kirk, Sarah R; Meyer, Scott C; Holman, Karen L McFarlane

    2015-01-01

    We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure, students work with computer modeling and visualization of myoglobin and its homologues, after which they spectroscopically characterize its thermal denaturation. Students also study protein function (ligand binding equilibrium) and are instructed on topics in data analysis (calibration curves, nonlinear vs. linear regression). This upper division biochemistry laboratory project is a challenging and rewarding one that not only exposes students to a wide variety of important biochemical laboratory techniques but also ties those techniques together to work with a single readily available and easily characterized protein, myoglobin.

  18. Modelling Rho GTPase biochemistry to predict collective cell migration

    NASA Astrophysics Data System (ADS)

    Merchant, Brian; Feng, James

    The collective migration of cells, due to individual cell polarization and intercellular contact inhibition of locomotion, features prominently in embryogenesis and metastatic cancers. Existing methods for modelling collectively migrating cells tend to rely either on highly abstracted agent-based models, or on continuum approximations of the group. Both of these frameworks represent intercellular interactions such as contact inhibition of locomotion as hard-coded rules defining model cells. In contrast, we present a vertex-dynamics framework which predicts polarization and contact inhibition of locomotion naturally from an underlying model of Rho GTPase biochemistry and cortical mechanics. We simulate the interaction between many such model cells, and study how modulating Rho GTPases affects migratory characteristics of the group, in the context of long-distance collective migration of neural crest cells during embryogenesis.

  19. Oxidative stress in marine environments: biochemistry and physiological ecology.

    PubMed

    Lesser, Michael P

    2006-01-01

    Oxidative stress-the production and accumulation of reduced oxygen intermediates such as superoxide radicals, singlet oxygen, hydrogen peroxide, and hydroxyl radicals-can damage lipids, proteins, and DNA. Many disease processes of clinical interest and the aging process involve oxidative stress in their underlying etiology. The production of reactive oxygen species is also prevalent in the world's oceans, and oxidative stress is an important component of the stress response in marine organisms exposed to a variety of insults as a result of changes in environmental conditions such as thermal stress, exposure to ultraviolet radiation, or exposure to pollution. As in the clinical setting, reactive oxygen species are also important signal transduction molecules and mediators of damage in cellular processes, such as apoptosis and cell necrosis, for marine organisms. This review brings together the voluminous literature on the biochemistry and physiology of oxidative stress from the clinical and plant physiology disciplines with the fast-increasing interest in oxidative stress in marine environments.

  20. Audio podcasting in a tablet PC-enhanced biochemistry course.

    PubMed

    Lyles, Heather; Robertson, Brian; Mangino, Michael; Cox, James R

    2007-11-01

    This report describes the effects of making audio podcasts of all lectures in a large, basic biochemistry course promptly available to students. The audio podcasts complement a previously described approach in which a tablet PC is used to annotate PowerPoint slides with digital ink to produce electronic notes that can be archived. The fundamentals of this approach are described, and data from student attitudinal and informational surveys are presented. The survey data suggest that the students have a positive attitude toward the combination of tablet-based instruction and audio podcasting. In addition, three students provide testimonials on how these technological tools allowed them to utilize their preferred learning styles to succeed in the course. Possible negative consequences of this approach, in terms of class attendance and note taking, are also analyzed and discussed.

  1. An in silico DNA cloning experiment for the biochemistry laboratory.

    PubMed

    Elkins, Kelly M

    2011-01-01

    This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced high school biology classes. Students begin by examining a plasmid map with the goal of identifying which restriction enzymes may be used to clone a piece of foreign DNA containing a gene of interest into the vector. From the National Center for Biotechnology Initiative website, students are instructed to retrieve a protein sequence and use Expasy's Reverse Translate program to reverse translate the protein to cDNA. Students then use Integrated DNA Technologies' OligoAnalyzer to predict the complementary DNA strand and obtain DNA recognition sequences for the desired restriction enzymes from New England Biolabs' website. Students add the appropriate DNA restriction sequences to the double-stranded foreign DNA for cloning into the plasmid and infecting Escherichia coli cells. Students are introduced to computational biology tools, molecular biology terminology and the process of DNA cloning in this valuable single session, in silico experiment. This project develops students' understanding of the cloning process as a whole and contrasts with other laboratory and internship experiences in which the students may be involved in only a piece of the cloning process/techniques. Students interested in pursuing postgraduate study and research or employment in an academic biochemistry or molecular biology laboratory or industry will benefit most from this experience.

  2. Hematologic and plasma biochemistry values in white storks (Ciconia ciconia).

    PubMed

    Szabó, Zoltán; Beregi, Attila; Vajdovich, Péter; Abonyi-Tóth, Zsolt; Mátrai, Eszter; Pazár, Péter; Gaál, Tibor

    2010-03-01

    Hematologic and plasma biochemistry parameters of the white stork (Ciconia ciconia) were studied. Blood samples were taken from a total of 80 adult white storks kept in captivity in Hungarian zoos and bird repatriation stations, between 2002 and 2006. Hematologic (packed cell volume, 46.3% +/- 5.3%; hemoglobin concentration, 127.8 +/- 20.4 g/L; red blood cell counts, 2.28 +/- 0.35 10(12)/l/l; white blood cell counts, 21.6 +/- 4.2 10(9)/l/ l; heterophils, 61.0% +/- 9.8% [13.1 +/- 3.2 x 10(9)/L]; lymphocytes, 34.3% +/- 9.1% [7.4 +/- 2.5 x 10(9)/L]; monocytes, 3.44% +/- 2.3% [0.78 +/- 0.57 x 10(9)/L]; eosinophils 0.75% +/- 0.91% [0.16 +/- 0.21 x 10(9)/L]; basophils 0.38% +/- 0.56% [0.04 +/- 0.07 x 10(9)/L]) and plasma biochemistry values (aspartate aminotransferase, 267.5 +/- 145.8 U/L; L-gamma-glutamyltransferase, 47.6 +/- 49.3 U/L; lipase, 70.3 +/- 60.6 U/L; creatine kinase, 443.9 +/- 182.2 U/L; lactate dehydrogenase, 880.4 +/- 293.6 U/L; alkaline phosphatase, 177.5 +/- 116.6 U/L; amylase, 917.6 +/- 314.3 U/L; glutamate dehydrogenase, 7.3 +/- 4.0 U/L; total protein, 45.2 +/- 8.1 g/L; uric acid, 459.2 +/- 254.3 micromol/L; and bile acids, 46.3 +/- 20.5 micromol/L) were determined. The results obtained can be used as reference values, because there are no established values previously reported for adult white storks.

  3. An in silico DNA cloning experiment for the biochemistry laboratory.

    PubMed

    Elkins, Kelly M

    2011-01-01

    This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced high school biology classes. Students begin by examining a plasmid map with the goal of identifying which restriction enzymes may be used to clone a piece of foreign DNA containing a gene of interest into the vector. From the National Center for Biotechnology Initiative website, students are instructed to retrieve a protein sequence and use Expasy's Reverse Translate program to reverse translate the protein to cDNA. Students then use Integrated DNA Technologies' OligoAnalyzer to predict the complementary DNA strand and obtain DNA recognition sequences for the desired restriction enzymes from New England Biolabs' website. Students add the appropriate DNA restriction sequences to the double-stranded foreign DNA for cloning into the plasmid and infecting Escherichia coli cells. Students are introduced to computational biology tools, molecular biology terminology and the process of DNA cloning in this valuable single session, in silico experiment. This project develops students' understanding of the cloning process as a whole and contrasts with other laboratory and internship experiences in which the students may be involved in only a piece of the cloning process/techniques. Students interested in pursuing postgraduate study and research or employment in an academic biochemistry or molecular biology laboratory or industry will benefit most from this experience. PMID:21618385

  4. Minimal impact of organic chemistry prerequisite on student performance in introductory biochemistry.

    PubMed

    Wright, Robin; Cotner, Sehoya; Winkel, Amy

    2009-01-01

    Curriculum design assumes that successful completion of prerequisite courses will have a positive impact on student performance in courses that require the prerequisite. We recently had the opportunity to test this assumption concerning the relationship between completion of the organic chemistry prerequisite and performance in introductory biochemistry. We found no statistically significant differences between average biochemistry grades or grade distribution among students with or without the organic chemistry prerequisite. However, students who had not completed the organic chemistry prerequisite before biochemistry were more likely to withdraw from the course than those who had completed the prerequisite. In contrast to the lack of correlation between performance in biochemistry and completion of organic chemistry, we observed a strong, highly significant positive relationship between cumulative GPA and the biochemistry grade. Our data suggest that excluding students without organic chemistry would have less positive impact on student success in biochemistry than would providing additional support for all students who enroll in biochemistry with a cumulative GPA below 2.5.

  5. Minimal Impact of Organic Chemistry Prerequisite on Student Performance in Introductory Biochemistry

    PubMed Central

    Cotner, Sehoya; Winkel, Amy

    2009-01-01

    Curriculum design assumes that successful completion of prerequisite courses will have a positive impact on student performance in courses that require the prerequisite. We recently had the opportunity to test this assumption concerning the relationship between completion of the organic chemistry prerequisite and performance in introductory biochemistry. We found no statistically significant differences between average biochemistry grades or grade distribution among students with or without the organic chemistry prerequisite. However, students who had not completed the organic chemistry prerequisite before biochemistry were more likely to withdraw from the course than those who had completed the prerequisite. In contrast to the lack of correlation between performance in biochemistry and completion of organic chemistry, we observed a strong, highly significant positive relationship between cumulative GPA and the biochemistry grade. Our data suggest that excluding students without organic chemistry would have less positive impact on student success in biochemistry than would providing additional support for all students who enroll in biochemistry with a cumulative GPA below 2.5. PMID:19255135

  6. Structure Leads To Function: An Integrated Biophysical Approach To Teaching a Biochemistry Laboratory.

    ERIC Educational Resources Information Center

    And Others; deLannoy, Peter

    1996-01-01

    Describes an integrated approach to teaching a biochemistry laboratory focusing on the relationship between the three-dimensional structure of a macromolecule and its function. RNA is chosen as the model system. Discusses curriculum and student assessment. (AIM)

  7. An Experiment Using Sucrose Density Gradients in the Undergraduate Biochemistry Laboratory.

    ERIC Educational Resources Information Center

    Turchi, Sandra L.; Weiss, Monica

    1988-01-01

    Describes an experiment to be performed in an undergraduate biochemistry laboratory that is based on a gradient centrifugation system employing a simple bench top centrifuge, a freezer, and frozen surcose gradient solution to separate macromolecules and subcellular components. (CW)

  8. Titration of Alanine Monitored by NMR Spectroscopy: A Biochemistry Laboratory Experiment

    ERIC Educational Resources Information Center

    Waller, Francis J.; And Others

    1977-01-01

    The experiment described here involves simultaneous monitoring of pH and NMR chemical shifts during an aqueous titration of alpha- and beta-alanine. This experiment is designed for use in an undergraduate biochemistry course. (MR)

  9. Learning Biochemistry through Manga--Helping Students Learn and Remember, and Making Lectures More Exciting.

    ERIC Educational Resources Information Center

    Nagata, Ryoichi

    1999-01-01

    Uses panels taken from manga, Japanese comics and cartoons, to supplement explanations of biochemical terms and topics in biochemistry classes. Results indicate that the use of manga helped students remember what they had learned. (Author/CCM)

  10. Teaching arrangements of carbohydrate metabolism in biochemistry curriculum in Peking University Health Science Center.

    PubMed

    Chen, Hao; Ni, Ju-Hua

    2013-01-01

    Biochemistry occupies a unique place in the medical school curricula, but the teaching of biochemistry presents certain challenges. One of these challenges is facilitating students' interest in and mastery of metabolism. The many pathways and modes of regulation can be overwhelming for students to learn and difficult for professors to teach in an engaging manner. The first chapter of the metabolism section in current Chinese biochemistry textbooks covers carbohydrate metabolism. Medical students usually complain about the difficulty of this subject. Here we discuss how to facilitate learning by rearranging the subjects in this introductory chapter of biochemical metabolism and to lay a solid foundation for future study. The strategy involves reorganizing the order in which subjects are taught from simple to complex and from short to long metabolic pathways. Most students taking the curriculum consider that the strategy engages their learning interests in biochemistry and enhances their learning outcomes.

  11. Foundational concepts and underlying theories for majors in "biochemistry and molecular biology".

    PubMed

    Tansey, John T; Baird, Teaster; Cox, Michael M; Fox, Kristin M; Knight, Jennifer; Sears, Duane; Bell, Ellis

    2013-01-01

    Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members and science educators from around the country that focused on identifying: 1) core principles of biochemistry and molecular biology, 2) essential concepts and underlying theories from physics, chemistry, and mathematics, and 3) foundational skills that undergraduate majors in biochemistry and molecular biology must understand to complete their major coursework. Using information gained from these workshops, as well as from the ASBMB accreditation working group and the NSF Vision and Change report, the Core Concepts working group has developed a consensus list of learning outcomes and objectives based on five foundational concepts (evolution, matter and energy transformation, homeostasis, information flow, and macromolecular structure and function) that represent the expected conceptual knowledge base for undergraduate degrees in biochemistry and molecular biology. This consensus will aid biochemistry and molecular biology educators in the development of assessment tools for the new ASBMB recommended curriculum.

  12. Development of an Integrated Laboratory Course-Research Program in Biochemistry

    ERIC Educational Resources Information Center

    Jones, Theodore H. D.

    1976-01-01

    Describes an experimental biochemistry course designed to teach basic biochemical techniques using an unstudied enzyme as the experimental material. Explains the schedule of experiments designed for the course and lists the sources used. (GS)

  13. Biochemical Markers of Bone Turnover Part I: Biochemistry and Variability

    PubMed Central

    2005-01-01

    With the ageing population in most countries, disorders of bone and mineral metabolism are becoming increasingly relevant to every day clinical practice. Consequently, the interest in, and the need for effective measures to be used in the screening, diagnosis and follow-up of such pathologies has markedly grown. Together with clinical and imaging techniques, biochemical tests play an important role in the assessment and differential diagnosis of metabolic bone disease. In recent years, the isolation and characterisation of cellular and extracellular components of the skeletal matrix have resulted in the development of molecular markers that are considered to reflect either bone formation or bone resorption. These biochemical indices are non-invasive, comparatively inexpensive and, when applied and interpreted correctly, helpful tools in the diagnostic and therapeutic assessment of metabolic bone disease. Part I of this article provides an overview of the basic biochemistry of bone markers, and sources of non-specific variability. Part II (to be published in a subsequent issue of this journal) will review the current evidence regarding the clinical use of biochemical markers of bone remodelling in metabolic and metastatic bone disease. PMID:16648882

  14. Effects of spaceflight on rat humerus geometry, biomechanics, and biochemistry

    NASA Technical Reports Server (NTRS)

    Vailas, A. C.; Zernicke, R. F.; Grindeland, R. E.; Kaplansky, A.; Durnova, G. N.; Li, K. C.; Martinez, D. A.

    1990-01-01

    The effects of a 12.5-day spaceflight (Cosmos 1887 biosatellite) on the geometric, biomechanical, and biochemical characteristics of humeri of male specific pathogen-free rats were examined. Humeri of age-matched basal control, synchronous control, and vivarium control rats were contrasted with the flight bones to examine the influence of growth and space environment on bone development. Lack of humerus longitudinal growth occurred during the 12.5 days in spaceflight. In addition, the normal mid-diaphysial periosteal appositional growth was affected; compared with their controls, the spaceflight humeri had less cortical cross-sectional area, smaller periosteal circumferences, smaller anterior-posterior periosteal diameters, and smaller second moments of area with respect to the bending and nonbending axes. The flexural rigidity of the flight humeri was comparable to that of the younger basal control rats and significantly less than that of the synchronous and vivarium controls; the elastic moduli of all four groups, nonetheless, were not significantly different. Generally, the matrix biochemistry of the mid-diaphysial cross sections showed no differences among groups. Thus, the spaceflight differences in humeral mechanical strength and flexural rigidity were probably a result of the differences in humeral geometry rather than material properties.

  15. Mapping intracellular biochemistry with fluorescence anisotropy imaging microscopy

    NASA Astrophysics Data System (ADS)

    Gough, Albert H.; Taylor, D. Lansing

    1994-08-01

    Fluorescence polarization anisotropy can be used to determine the rotational mobility of a fluorescent analog, detect anisotropic orientation distributions, or measure the fluorescence lifetime of a fluorophore. Steady state fluorescence anisotropy can be simply measured in a standard fluorescence microscope equipped with excitation and emission polarizers and therefore, two dimensional maps of fluorescence anisotropy can be easily acquired. We are using steady state fluorescence anisotropy imaging microscopy to study the biochemistry of cell motility. The optimum fluorophore for fluorescence polarization measurements has a fluorescence lifetime that is comparable to the rotational correlation time of the molecule of interest. In order to make imaging measurements with high sensitivity and reasonable time resolution, however, this general rule has to be adjusted, and we have found that FITC-calmodulin has a useful combination of the above features. Calmodulin is a key regulatory protein, that is proposed to be involved in the regulation of the actin-myosin II based force generation in non-muscle cells. The rotational mobility of macromolecules is very sensitive to molecular interactions, yet is relatively insensitive to any surrounding gel matrix. We have taken advantage of this feature to map FITC-calmodulin interactions in the complex cytomatrix in living cells by steady state Fluorescence Anisotropy Imaging Microscopy (FAIM). In addition, we are investigating the use of FAIM for mapping variations in molecular orientation distributions, and fluorescence lifetime distributions.

  16. The face of Latin American comparative biochemistry and physiology.

    PubMed

    Hermes-Lima, Marcelo; Navas, Carlos A

    2006-01-01

    Latin America has experienced a vigorous growth in scientific production since the 1990s, rising from 1.7% of the world's share of science publications in 1990 to 3.2% in 2000. This appears to be a consequence of increasing investment in tertiary education, especially at the doctoral level. However, such growth is not homogeneous among the nations of Latin America, being affected by many issues, such as economical power and expenses in science and technology. Biology--including comparative biochemistry and physiology (CBP)--is one of the scientific areas of tremendous growth in the continent. Thus, in order to celebrate the increasing participation of Latin America in the field of CBP, the editorial board of Comp. Biochem. Physiol. decided to organize a special volume dedicated to Latin American authors (CBP-Latin America). From May to November 2005, 52 manuscripts were submitted to CBP-Latin America from Argentina, Brazil, Chile, Cuba, Mexico, Uruguay and Venezuela. This opening issue contains the first two dozen manuscripts, highlighting the diversity of experimental approaches and the breadth and uniqueness of the biological systems available to researchers in Latin America. We hope that the CBP-Latin America project becomes a significant editorial initiative, one that will meet the goals of highlighting, integrating, and mapping CBP research in Latin America.

  17. Contributions of nuclear magnetic resonance to renal biochemistry

    SciTech Connect

    Ross, B.; Freeman, D.; Chan, L.

    1986-01-01

    /sup 31/P NMR as a descriptive technique is of interest to nephrologists. Particular contributions of /sup 31/P NMR to our understanding of renal function may be enumerated.: Free metabolite levels are different from those classically accepted; in particular, ADP and Pi are low with implications for the control of renal metabolism and Pi transport, and, via the phosphorylation potential, for Na+ transport. Renal pH is heterogeneous; between cortex, outer medulla, and papilla, and between cell and lumen, a large pH gradient exists. Also, quantitation between cytosol and mitochondrion of the pH gradient is now feasible. In acute renal failure of either ischemic or nonischemic origin, both ATP depletion and acidification of the renal cell result in damage, with increasing evidence for the importance of the latter. Measurements of renal metabolic rate in vivo suggest the existence of a prodromal phase of acute renal failure, which could lead to its detection at an earlier and possibly reversible stage. Human renal cancers show a unique /sup 31/P NMR spectrum and a very acidic environment. Cancer chemotherapy may alter this and detection of such changes with NMR offers a method of therapeutic monitoring with significance beyond nephrology. Renal cortex and medulla have a different T1 relaxation time, possibly due to differences in lipid composition. It seems that NMR spectroscopy has much to offer to the future understanding of the relationship between renal biochemistry and function. 56 references.

  18. Bitter taste receptors: Novel insights into the biochemistry and pharmacology.

    PubMed

    Jaggupilli, Appalaraju; Howard, Ryan; Upadhyaya, Jasbir D; Bhullar, Rajinder P; Chelikani, Prashen

    2016-08-01

    Bitter taste receptors (T2Rs) belong to the super family of G protein-coupled receptors (GPCRs). There are 25 T2Rs expressed in humans, and these interact with a large and diverse group of bitter ligands. T2Rs are expressed in many extra-oral tissues and can perform diverse physiological roles. Structure-function studies led to the identification of similarities and dissimilarities between T2Rs and Class A GPCRs including amino acid conservation and novel motifs. However, the efficacy of most of the T2R ligands is not yet elucidated and the biochemical pharmacology of T2Rs is poorly understood. Recent studies on T2Rs characterized novel ligands including blockers for these receptors that include inverse agonist and antagonists. In this review we discuss the techniques used for elucidating bitter blockers, concept of ligand bias, generic amino acid numbering, the role of cholesterol, and conserved water molecules in the biochemistry and pharmacology of T2Rs. PMID:26995065

  19. Biochemistry and physiology of anabolic androgenic steroids doping.

    PubMed

    Lippi, G; Franchini, M; Banfi, G

    2011-05-01

    Anabolic Androgenic Steroids (AASs) are chemical and pharmacological derivatives of the male hormone testosterone which are widely used for increasing burst and sprinting activities in sports. Although AASs are thought to be transversal to the plurality of sports disciplines, they are principally misused by bodybuilders, weightlifters, shot, hammer, discus or javelin throwers, rugby and American football players as well as by swimmers and runners. AAS exert a kaleidoscope of effects on human biology, principally through the 5-α-reductase-mediated conversion into dihydrotestosterone, the aromatase-mediated conversion into female sex hormones, a competitive antagonism to the glucocorticoid receptors, the potential stimulation of erythropoietin secretion as well as psychoactive effects on the brain. The influence of AASs on physical performance is still undefined, since the large number of studies published so far have described discordant and often contradictory outcomes. Nevertheless, animal and human investigations support the hypothesis that the administration of AASs might increase lean body mass, muscle mass, and maximal voluntary strength especially in men, so that they would represent an appealing form of doping for increasing power capacity, sustaining intensive training periods and, last but not least, as a cosmetic muscle makeover. The aim of this article is to review the biochemistry, physiology and the ergogenic effects of AASs.

  20. Tryptophan Biochemistry: Structural, Nutritional, Metabolic, and Medical Aspects in Humans

    PubMed Central

    Palego, Lionella; Betti, Laura; Rossi, Alessandra; Giannaccini, Gino

    2016-01-01

    L-Tryptophan is the unique protein amino acid (AA) bearing an indole ring: its biotransformation in living organisms contributes either to keeping this chemical group in cells and tissues or to breaking it, by generating in both cases a variety of bioactive molecules. Investigations on the biology of Trp highlight the pleiotropic effects of its small derivatives on homeostasis processes. In addition to protein turn-over, in humans the pathways of Trp indole derivatives cover the synthesis of the neurotransmitter/hormone serotonin (5-HT), the pineal gland melatonin (MLT), and the trace amine tryptamine. The breakdown of the Trp indole ring defines instead the “kynurenine shunt” which produces cell-response adapters as L-kynurenine, kynurenic and quinolinic acids, or the coenzyme nicotinamide adenine dinucleotide (NAD+). This review aims therefore at tracing a “map” of the main molecular effectors in human tryptophan (Trp) research, starting from the chemistry of this AA, dealing then with its biosphere distribution and nutritional value for humans, also focusing on some proteins responsible for its tissue-dependent uptake and biotransformation. We will thus underscore the role of Trp biochemistry in the pathogenesis of human complex diseases/syndromes primarily involving the gut, neuroimmunoendocrine/stress responses, and the CNS, supporting the use of -Omics approaches in this field. PMID:26881063

  1. Engagement of students with lectures in biochemistry and pharmacology.

    PubMed

    Davis, Elizabeth Ann; Hodgson, Yvonne; Macaulay, Janet Olwyn

    2012-01-01

    Academic staff at universities have become concerned about the decrease in student attendance at lectures and the implication of this on student achievement and learning. Few studies have measured actual lecture attendance in a coherent or comprehensive way. The aim of this study was to measure actual lecture attendance of students over two year levels enrolled in two separate science disciplines, biochemistry and pharmacology. The study further sought to determine the factors that influence lecture attendance. Attendance at lectures in four units of study was monitored over a 12-week semester. Attendance at lectures decreased over the semester and was lower at early morning lectures (8 A.M.; 9 A.M.). A questionnaire surveying students about their preparation for lectures, their compensation for missed lectures and the factors influencing their nonattendance was administered at the end of the semester. Students reported that the major factors influencing their attendance at lectures related to timetable issues and the quality of lecturing. If students missed lectures, the majority read the lecture notes and listened to the online recordings. The availability of online recordings of lectures was not a major influence on attendance at lectures. In three of the four units studied there was no correlation between self-reported lecture attendance and exam performance. The results of the study indicate that universities should dedicate more resources to timetabling and to supporting staff to improve the quality of their lectures.

  2. A synthetic biochemistry module for production of bio-based chemicals from glucose.

    PubMed

    Opgenorth, Paul H; Korman, Tyler P; Bowie, James U

    2016-06-01

    Synthetic biochemistry, the cell-free production of biologically based chemicals, is a potentially high-yield, flexible alternative to in vivo metabolic engineering. To limit costs, cell-free systems must be designed to operate continuously with minimal addition of feedstock chemicals. We describe a robust, efficient synthetic glucose breakdown pathway and implement it for the production of bioplastic. The system's performance suggests that synthetic biochemistry has the potential to become a viable industrial alternative.

  3. A synthetic biochemistry module for production of bio-based chemicals from glucose.

    PubMed

    Opgenorth, Paul H; Korman, Tyler P; Bowie, James U

    2016-06-01

    Synthetic biochemistry, the cell-free production of biologically based chemicals, is a potentially high-yield, flexible alternative to in vivo metabolic engineering. To limit costs, cell-free systems must be designed to operate continuously with minimal addition of feedstock chemicals. We describe a robust, efficient synthetic glucose breakdown pathway and implement it for the production of bioplastic. The system's performance suggests that synthetic biochemistry has the potential to become a viable industrial alternative. PMID:27065234

  4. Need assessment of enhancing the weightage of applied biochemistry in the undergraduate curriculum at MGIMS, sevagram.

    PubMed

    Kumar, Satish; Jena, Lingaraja; Vagha, Jayant

    2016-05-01

    In order to review the need assessment of enhancing the weightage of Applied Biochemistry in the undergraduate curriculum at Mahatma Gandhi Institute of Medical Sciences (MGIMS), Sevagram, a validated questionnaire was sent to 453 participants which include 387 undergraduate students, 11 interns, 23 postgraduate students, and 32 faculty members. A web-based data collection and analysis tool was designed for online questionnaire distribution, data collection, and analysis. Response rate was 100%. Most of the respondents agreed that the subject Biochemistry has relevance in clinical practice (81.24%) and applied based learning of Biochemistry by medical undergraduates would help in overall improvement in the health standards/patients care (83.44%). According to 65.12% respondents, most of the medical undergraduates read Biochemistry just for examination purpose only. Nearly half of the respondents agreed that minute details of biochemical reactions were not much useful in clinical practice (53.86%) and the vast majority of diagrammatic cycles memorized by the medical undergraduates had no relevance in clinical practice (51.21%), the decreased interest in learning the Applied Biochemistry was due to more amount of clinically irrelevant information taught to medical undergraduates (73.51%), there was a need to rethink for removing the diagrammatic biochemical cycles from curriculum for medical undergraduates (48.12%), the less learning of Applied Biochemistry or competencies would affect the clinical skills and knowledge of medical undergraduates (70.42%). The result of this study suggests that there is need for restructuring the Biochemistry curriculum with more clinical relevance. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:230-240, 2016.

  5. Teaching Biochemistry at Lisbon University--Facing the Challenge of the Bologna Declaration in the 25th Anniversary of the Biochemistry Course

    ERIC Educational Resources Information Center

    Farinha, Carlos M.; Freire, Ana Ponces

    2007-01-01

    The biochemistry degree has been taught at Lisbon University for 25 years. Since its creation, the curriculum is characterized for being widely eclectic and multidisciplinary. The adoption of the concepts proposed in Europe by the Declaration of Bologna and incorporation of these ideas at Lisbon University is discussed here for the biochemistry…

  6. A Biochemistry and Molecular Biology Experiment and Evaluation System for Biotechnology Specialty Students: An Effective Evaluation System to Improve the Biochemistry and Molecular Biology Experiment Teaching

    ERIC Educational Resources Information Center

    Li, Suxia; Wu, Haizhen; Zhao, Jian; Ou, Ling; Zhang, Yuanxing

    2010-01-01

    In an effort to achieve high success in knowledge and technique acquisition as a whole, a biochemistry and molecular biology experiment was established for high-grade biotechnology specialty students after they had studied essential theory and received proper technique training. The experiment was based on cloning and expression of alkaline…

  7. Integrative Signaling Networks of Membrane Guanylate Cyclases: Biochemistry and Physiology

    PubMed Central

    Sharma, Rameshwar K.; Duda, Teresa; Makino, Clint L.

    2016-01-01

    This monograph presents a historical perspective of cornerstone developments on the biochemistry and physiology of mammalian membrane guanylate cyclases (MGCs), highlighting contributions made by the authors and their collaborators. Upon resolution of early contentious studies, cyclic GMP emerged alongside cyclic AMP, as an important intracellular second messenger for hormonal signaling. However, the two signaling pathways differ in significant ways. In the cyclic AMP pathway, hormone binding to a G protein coupled receptor leads to stimulation or inhibition of an adenylate cyclase, whereas the cyclic GMP pathway dispenses with intermediaries; hormone binds to an MGC to affect its activity. Although the cyclic GMP pathway is direct, it is by no means simple. The modular design of the molecule incorporates regulation by ATP binding and phosphorylation. MGCs can form complexes with Ca2+-sensing subunits that either increase or decrease cyclic GMP synthesis, depending on subunit identity. In some systems, co-expression of two Ca2+ sensors, GCAP1 and S100B with ROS-GC1 confers bimodal signaling marked by increases in cyclic GMP synthesis when intracellular Ca2+ concentration rises or falls. Some MGCs monitor or are modulated by carbon dioxide via its conversion to bicarbonate. One MGC even functions as a thermosensor as well as a chemosensor; activity reaches a maximum with a mild drop in temperature. The complexity afforded by these multiple limbs of operation enables MGC networks to perform transductions traditionally reserved for G protein coupled receptors and Transient Receptor Potential (TRP) ion channels and to serve a diverse array of functions, including control over cardiac vasculature, smooth muscle relaxation, blood pressure regulation, cellular growth, sensory transductions, neural plasticity and memory. PMID:27695398

  8. Integrative Signaling Networks of Membrane Guanylate Cyclases: Biochemistry and Physiology

    PubMed Central

    Sharma, Rameshwar K.; Duda, Teresa; Makino, Clint L.

    2016-01-01

    This monograph presents a historical perspective of cornerstone developments on the biochemistry and physiology of mammalian membrane guanylate cyclases (MGCs), highlighting contributions made by the authors and their collaborators. Upon resolution of early contentious studies, cyclic GMP emerged alongside cyclic AMP, as an important intracellular second messenger for hormonal signaling. However, the two signaling pathways differ in significant ways. In the cyclic AMP pathway, hormone binding to a G protein coupled receptor leads to stimulation or inhibition of an adenylate cyclase, whereas the cyclic GMP pathway dispenses with intermediaries; hormone binds to an MGC to affect its activity. Although the cyclic GMP pathway is direct, it is by no means simple. The modular design of the molecule incorporates regulation by ATP binding and phosphorylation. MGCs can form complexes with Ca2+-sensing subunits that either increase or decrease cyclic GMP synthesis, depending on subunit identity. In some systems, co-expression of two Ca2+ sensors, GCAP1 and S100B with ROS-GC1 confers bimodal signaling marked by increases in cyclic GMP synthesis when intracellular Ca2+ concentration rises or falls. Some MGCs monitor or are modulated by carbon dioxide via its conversion to bicarbonate. One MGC even functions as a thermosensor as well as a chemosensor; activity reaches a maximum with a mild drop in temperature. The complexity afforded by these multiple limbs of operation enables MGC networks to perform transductions traditionally reserved for G protein coupled receptors and Transient Receptor Potential (TRP) ion channels and to serve a diverse array of functions, including control over cardiac vasculature, smooth muscle relaxation, blood pressure regulation, cellular growth, sensory transductions, neural plasticity and memory.

  9. Biochemistry and Evolution of Anaerobic Energy Metabolism in Eukaryotes

    PubMed Central

    Müller, Miklós; Mentel, Marek; van Hellemond, Jaap J.; Henze, Katrin; Woehle, Christian; Gould, Sven B.; Yu, Re-Young; van der Giezen, Mark

    2012-01-01

    Summary: Major insights into the phylogenetic distribution, biochemistry, and evolutionary significance of organelles involved in ATP synthesis (energy metabolism) in eukaryotes that thrive in anaerobic environments for all or part of their life cycles have accrued in recent years. All known eukaryotic groups possess an organelle of mitochondrial origin, mapping the origin of mitochondria to the eukaryotic common ancestor, and genome sequence data are rapidly accumulating for eukaryotes that possess anaerobic mitochondria, hydrogenosomes, or mitosomes. Here we review the available biochemical data on the enzymes and pathways that eukaryotes use in anaerobic energy metabolism and summarize the metabolic end products that they generate in their anaerobic habitats, focusing on the biochemical roles that their mitochondria play in anaerobic ATP synthesis. We present metabolic maps of compartmentalized energy metabolism for 16 well-studied species. There are currently no enzymes of core anaerobic energy metabolism that are specific to any of the six eukaryotic supergroup lineages; genes present in one supergroup are also found in at least one other supergroup. The gene distribution across lineages thus reflects the presence of anaerobic energy metabolism in the eukaryote common ancestor and differential loss during the specialization of some lineages to oxic niches, just as oxphos capabilities have been differentially lost in specialization to anoxic niches and the parasitic life-style. Some facultative anaerobes have retained both aerobic and anaerobic pathways. Diversified eukaryotic lineages have retained the same enzymes of anaerobic ATP synthesis, in line with geochemical data indicating low environmental oxygen levels while eukaryotes arose and diversified. PMID:22688819

  10. Reef facies distribution patterns, Pleistocene (125 Ka) Falmouth Formation, Rio Bueno, Jamaica, W. I

    SciTech Connect

    Precht, W.F. ); Hoyt, W.H. )

    1991-03-01

    Detailed paleoecologic and sedimentologic studies of the well-exposed, Pleistocene (125 Ka) Falmouth Formation from Rio Bueno, Jamaica, where undertaken to define both temporal and spatial changes in reef architecture. Analyses of samples reveal an overall shallowing - upwards motif and a distinct lateral zonation of reefal facies similar to those observed in Recent fringing-reef and bank-barrier reef complexes from the eastern and western sides of Discovery Bay, Jamaica, respectively. The Falmouth Formation that crops out on the eastern shore of Rio Bueno Harbor is continually exposed (north-south) for approximately 0.5 km in length. The top of the reef exposure is approximately +4.5 m above MSL. This height correlates directly with sea level maxima for the Sangamon in Jamaica based upon a wavecut notch in the Hopegate Formation at the top of the Falmouth onlap surface. Interpretation of these deposits indicates that no lagoon or back-reef facies were present and that there was a gradation of energy regimes from high-to-low, north-to-south within this true fringing reef complex. Detailed sedimentologic analysis of thin-sections from all the above lithologies confirms the aforementioned paleoenvironmental interpretations. This study emphasizes the usefulness of modern counterparts in Pleistocene reef analysis and interpretation, and allows for an understanding of temporal (vertical) and spatial (horizontal) variations due to both physical disturbance and local sea level history that are preserved in these reefal deposits.

  11. Known structure, unknown function: An inquiry‐based undergraduate biochemistry laboratory course

    PubMed Central

    Gray, Cynthia; Price, Carol W.; Lee, Christopher T.; Dewald, Alison H.; Cline, Matthew A.; McAnany, Charles E.

    2015-01-01

    Abstract Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry‐ and research‐based could simultaneously impart scientific knowledge and foster a student's research expertise and confidence. We have developed a year‐long undergraduate biochemistry laboratory curriculum wherein students determine, via experiment and computation, the function of a protein of known three‐dimensional structure. The first half of the course is inquiry‐based and modular in design; students learn general biochemical techniques while gaining preparation for research experiments in the second semester. Having learned standard biochemical methods in the first semester, students independently pursue their own (original) research projects in the second semester. This new curriculum has yielded an improvement in student performance and confidence as assessed by various metrics. To disseminate teaching resources to students and instructors alike, a freely accessible Biochemistry Laboratory Education resource is available at http://biochemlab.org. © 2015 The Authors Biochemistry and Molecular Biology Education published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology, 43(4):245–262, 2015. PMID:26148241

  12. Teaching and learning of medical biochemistry according to clinical realities: A case study.

    PubMed

    Jabaut, Joshua M; Dudum, Ramzi; Margulies, Samantha L; Mehta, Akshita; Han, Zhiyong

    2016-01-01

    To foster medical students to become physicians who will be lifelong independent learners and critical thinkers with healthy skepticism and provide high-quality patient care guided by the best evidence, teaching of evidence-based medicine (EBM) has become an important component of medical education. Currently, the teaching and learning of biochemistry in medical schools incorporates its medical relevance and applications. However, to our knowledge there have been no reports on integrating EBM with teaching and learning medical biochemistry. Here, we present a case study to illustrate the significance of this approach. This case study was based on a biochemistry/nutrition question in a popular board review book about whether a homeless alcoholic man is at risk of developing a deficiency of vitamin E. The possible answers and explanation provided in the book raised a question about the correct answer, which provided us with an opportunity to adapt the philosophy and certain basic EBM principles to find evidence for the clinical applicability of a commonly taught biochemistry topic. The outcome of this case study not only taught us how to conduct an EBM exercise to answer a specific patient question, but also provided us with an opportunity for in-depth teaching and learning of the medical relevance of a specific biochemistry topic based on the best clinical evidence obtained from a systematic research of medical literature. PMID:26593685

  13. Teaching and learning of medical biochemistry according to clinical realities: A case study.

    PubMed

    Jabaut, Joshua M; Dudum, Ramzi; Margulies, Samantha L; Mehta, Akshita; Han, Zhiyong

    2016-01-01

    To foster medical students to become physicians who will be lifelong independent learners and critical thinkers with healthy skepticism and provide high-quality patient care guided by the best evidence, teaching of evidence-based medicine (EBM) has become an important component of medical education. Currently, the teaching and learning of biochemistry in medical schools incorporates its medical relevance and applications. However, to our knowledge there have been no reports on integrating EBM with teaching and learning medical biochemistry. Here, we present a case study to illustrate the significance of this approach. This case study was based on a biochemistry/nutrition question in a popular board review book about whether a homeless alcoholic man is at risk of developing a deficiency of vitamin E. The possible answers and explanation provided in the book raised a question about the correct answer, which provided us with an opportunity to adapt the philosophy and certain basic EBM principles to find evidence for the clinical applicability of a commonly taught biochemistry topic. The outcome of this case study not only taught us how to conduct an EBM exercise to answer a specific patient question, but also provided us with an opportunity for in-depth teaching and learning of the medical relevance of a specific biochemistry topic based on the best clinical evidence obtained from a systematic research of medical literature.

  14. On the Chemical Emergence of Phosphate-Based Biochemistry

    NASA Astrophysics Data System (ADS)

    Kee, Terence

    Contemporary organisms use orthophosphate derivatives (PO43-) in their cell biochemistry,1 yet questions remain as to how Nature was able to accumulate, activate and exploit the or-thophosphate group from geological sources with both poorly solubility and low chemical activ-ity.2 Gulick argued3 a central role for reduced oxidation state phosphorus (P) oxyacids such as H-phosphonates (H2PO3-) and especially H-phosphinates (H2PO2-) in prebiotic chemistry on account of the greater water solubility of their metal salts and, with the presence of P-H bonds, a different reactivity profile to that expected of orthophosphate. The recent demonstration that hydrothermal corrosion of P-rich mineral phases such as schreibersite (Fe,Ni)3P within iron meteorites leads to production of various P-oxyacids including H-phosphonic (H3PO3)4 and H-phosphinic5 acids as well as orthophosphate has reignited interest in reduced oxida-tion state P chemistry in prebiotic environments. We are examining the prebiotic potential of reduced oxidation state P-chemistry through reactions with carbonyl substrates with rea-sonable prebiotic provenance including formaldehyde glycolaldehyde, both intimately involved in the formose reaction for sugar synthesis6 and pyruvic acid,7 a product of glycolysis and feed-stock for the citric acid cycle, a fundamental cellular metablic process whose heritage is considered an ancient one. In this contribution we present some of our latest results on the H-phosphinate-pyruvate system. References: [1] Lodish H et al. (2000) Molecular Cell Biology, 4th Ed., W. H. Freeman Co., New York. [2] Gulick A. (1955) Am. Sci., 43, 479. [3] Gulick A. (1957) Ann. N. Y. Acad. Sci. 69, 309. [4] Pasek M. A. (2008) Proc. Nat. Acad. Sci. USA, 105, 853. [5] Bryant D. E.and Kee T. P. (2006) Chem. Commun. 2344. [6] Weber A. L. (2000) Origins of Life and Evol. Biosph., 30, 33. [7] Cody G. D. et. al. (2000) Science 289, 1337.

  15. The Sublime Corpse in Gertrudis Gomez de Avellaneda's Women's Journal "Album Cubano de lo Bueno y lo Bello" (1860)

    ERIC Educational Resources Information Center

    LaGreca, Nancy

    2009-01-01

    This article examines Gertrudis Gomez de Avellaneda's choice to include articles depicting the advanced decay of cadavers, which are simultaneously horrible and awesome, in her women's periodical "Album Cubano de lo Bueno y lo Bello". Background on Avellaneda's biography, women's print culture, and theories of the sublime provide a frame for the…

  16. A synthetic biochemistry molecular purge valve module that maintains redox balance.

    PubMed

    Opgenorth, Paul H; Korman, Tyler P; Bowie, James U

    2014-01-01

    The greatest potential environmental benefit of metabolic engineering would be the production of high-volume commodity chemicals, such as biofuels. Yet, the high yields required for the economic viability of low-value chemicals is particularly hard to achieve in microbes owing to the myriad competing biochemical pathways. An alternative approach, which we call synthetic biochemistry, is to eliminate the organism by constructing biochemical pathways in vitro. Viable synthetic biochemistry, however, will require simple methods to replace the cellular circuitry that maintains cofactor balance. Here we design a simple purge valve module for maintaining NADP(+)/NADPH balance. We test the purge valve in the production of polyhydroxybutyryl bioplastic and isoprene--pathways where cofactor generation and utilization are unbalanced. We find that the regulatory system is highly robust to variations in cofactor levels and readily transportable. The molecular purge valve provides a step towards developing continuously operating, sustainable synthetic biochemistry systems.

  17. Utilizing Isolation, Purification, and Characterization of Enzymes as Project-Oriented Labs for Undergraduate Biochemistry

    NASA Astrophysics Data System (ADS)

    Deal, S. Todd; Hurst, Michael O.

    1997-02-01

    Senior-level biochemistry labs are mostly verification-type laboratories with little chance for exploration. We have developed a project-based biochemistry laboratory which gives them a chance to carry out a major biochemistry project. In the first quarter it is based on the purification of the enzyme lysozyme. The students are given some basic information, and then work out the details of their own procedures, make up their own solutions, and work at their own pace. Students use centrifugation, ion-exchange chromatography, spectral enzyme assays, and SDS-gel electrophoresis to purify and characterize the protein. In the second quarter students are given acid phosphatase and the basic assay for the enzyme, and then develop and carry out a method for determining the kinetic parameters of the enzyme. These experiments continue the development of laboratory independence of the students which steadily progresses in most curriculum

  18. The biochemistry of blister fluid from pediatric burn injuries: proteomics and metabolomics aspects.

    PubMed

    Zang, Tuo; Broszczak, Daniel A; Broadbent, James A; Cuttle, Leila; Lu, Haitao; Parker, Tony J

    2016-01-01

    Burn injury is a prevalent and traumatic event for pediatric patients. At present, the diagnosis of burn injury severity is subjective and lacks a clinically relevant quantitative measure. This is due in part to a lack of knowledge surrounding the biochemistry of burn injuries and that of blister fluid. A more complete understanding of the blister fluid biochemistry may open new avenues for diagnostic and prognostic development. Burn insult induces a highly complex network of signaling processes and numerous changes within various biochemical systems, which can ultimately be examined using proteome and metabolome measurements. This review reports on the current understanding of burn wound biochemistry and outlines a technical approach for 'omics' profiling of blister fluid from burn wounds of differing severity.

  19. Evolutionary biochemistry: revealing the historical and physical causes of protein properties

    PubMed Central

    Harms, Michael J.; Thornton, Joseph W.

    2014-01-01

    The repertoire of proteins and nucleic acids in the living world is determined by evolution; their properties are determined by the laws of physics and chemistry. Explanations of these two kinds of causality — the purviews of evolutionary biology and biochemistry, respectively — are typically pursued in isolation, but many fundamental questions fall squarely at the interface of fields. Here we articulate the paradigm of evolutionary biochemistry, which aims to dissect the physical mechanisms and evolutionary processes by which biological molecules diversified and to reveal how their physical architecture facilitates and constrains their evolution. We show how an integration of evolution with biochemistry moves us towards a more complete understanding of why biological molecules have the properties that they do. PMID:23864121

  20. A synthetic biochemistry molecular purge valve module that maintains redox balance.

    PubMed

    Opgenorth, Paul H; Korman, Tyler P; Bowie, James U

    2014-01-01

    The greatest potential environmental benefit of metabolic engineering would be the production of high-volume commodity chemicals, such as biofuels. Yet, the high yields required for the economic viability of low-value chemicals is particularly hard to achieve in microbes owing to the myriad competing biochemical pathways. An alternative approach, which we call synthetic biochemistry, is to eliminate the organism by constructing biochemical pathways in vitro. Viable synthetic biochemistry, however, will require simple methods to replace the cellular circuitry that maintains cofactor balance. Here we design a simple purge valve module for maintaining NADP(+)/NADPH balance. We test the purge valve in the production of polyhydroxybutyryl bioplastic and isoprene--pathways where cofactor generation and utilization are unbalanced. We find that the regulatory system is highly robust to variations in cofactor levels and readily transportable. The molecular purge valve provides a step towards developing continuously operating, sustainable synthetic biochemistry systems. PMID:24936528

  1. Biochemistry students' ideas about shape and charge in enzyme-substrate interactions.

    PubMed

    Linenberger, Kimberly J; Bretz, Stacey Lowery

    2014-01-01

    Biochemistry is a visual discipline that requires students to develop an understanding of numerous representations. However, there is very little known about what students actually understand about the representations that are used to communicate ideas in biochemistry. This study investigated biochemistry students' understanding of multiple representations of enzyme-substrate interactions through both student interviews (N = 25) and responses by a national sample (N = 707) to the Enzyme-Substrate Interactions Concept Inventory. This manuscript reports the findings regarding one category of misconceptions measured by the concept inventory, namely, students' understandings of shape and charge in the context of enzyme-substrate interactions. Students interpret molecular representations depicting such interactions by determining the complementarity between enzyme and substrate by focusing upon charge and hydrogen bonding, but with a disregard for stereochemistry.

  2. Essential concepts and underlying theories from physics, chemistry, and mathematics for "biochemistry and molecular biology" majors.

    PubMed

    Wright, Ann; Provost, Joseph; Roecklein-Canfield, Jennifer A; Bell, Ellis

    2013-01-01

    Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members from around the country. The workshops have focused on developing lists of Core Principles or Foundational Concepts in Biochemistry and Molecular Biology, a list of foundational skills, and foundational concepts from Physics, Chemistry, and Mathematics that all Biochemistry or Molecular Biology majors must understand to complete their major coursework. The allied fields working group created a survey to validate foundational concepts from Physics, Chemistry, and Mathematics identified from participant feedback at various workshops. One-hundred twenty participants responded to the survey and 68% of the respondents answered yes to the question: "We have identified the following as the core concepts and underlying theories from Physics, Chemistry, and Mathematics that Biochemistry majors or Molecular Biology majors need to understand after they complete their major courses: 1) mechanical concepts from Physics, 2) energy and thermodynamic concepts from Physics, 3) critical concepts of structure from chemistry, 4) critical concepts of reactions from Chemistry, and 5) essential Mathematics. In your opinion, is the above list complete?" Respondents also delineated subcategories they felt should be included in these broad categories. From the results of the survey and this analysis the allied fields working group constructed a consensus list of allied fields concepts, which will help inform Biochemistry and Molecular Biology educators when considering the ASBMB recommended curriculum for Biochemistry or Molecular Biology majors and in the development of appropriate assessment tools to gauge student understanding of how these concepts relate to biochemistry and molecular biology.

  3. Combining content and elements of communication into an upper-level biochemistry course.

    PubMed

    Whittington, Carli P; Pellock, Samuel J; Cunningham, Rebecca L; Cox, James R

    2014-01-01

    This report describes how a science communication module was incorporated into an advanced biochemistry course. Elements of communication were taught synergistically with biochemistry content in this course in an effort to expose students to a variety of effective oral communication strategies. Students were trained to use these established techniques and incorporated them into various presentations throughout the course. Three students describe their use of specific resources and how the skills learned relate to their future career. The importance and relevance of science communication are receiving unprecedented national attention. The academic scientific community must respond by incorporating more communication-centered instruction and opportunities in the classroom and laboratory.

  4. Design of a Comprehensive Biochemistry and Molecular Biology Experiment: Phase Variation Caused by Recombinational Regulation of Bacterial Gene Expression

    ERIC Educational Resources Information Center

    Sheng, Xiumei; Xu, Shungao; Lu, Renyun; Isaac, Dadzie; Zhang, Xueyi; Zhang, Haifang; Wang, Huifang; Qiao, Zheng; Huang, Xinxiang

    2014-01-01

    Scientific experiments are indispensable parts of Biochemistry and Molecular Biology. In this study, a comprehensive Biochemistry and Molecular Biology experiment about "Salmonella enterica" serovar Typhi Flagellar phase variation has been designed. It consisted of three parts, namely, inducement of bacterial Flagellar phase variation,…

  5. Reactivity I: A Foundation-Level Course for Both Majors and Nonmajors in Integrated Organic, Inorganic, and Biochemistry

    ERIC Educational Resources Information Center

    Schaller, Chris P.; Graham, Kate J.; Johnson, Brian J.; Jones, T. Nicholas; McIntee, Edward J.

    2015-01-01

    A foundation level course is presented that integrates aspects of organic, inorganic and biochemistry in the context of reactivity. The course was designed to serve majors in chemistry and other sciences (biochemistry, biology, nutrition), as well as nursing and pre-health professions students. Themes of the course were designed to highlight a…

  6. Diverse Assessment and Active Student Engagement Sustain Deep Learning: A Comparative Study of Outcomes in Two Parallel Introductory Biochemistry Courses

    ERIC Educational Resources Information Center

    Bevan, Samantha J.; Chan, Cecilia W. L.; Tanner, Julian A.

    2014-01-01

    Although there is increasing evidence for a relationship between courses that emphasize student engagement and achievement of student deep learning, there is a paucity of quantitative comparative studies in a biochemistry and molecular biology context. Here, we present a pedagogical study in two contrasting parallel biochemistry introductory…

  7. Air Pollution

    MedlinePlus

    Air pollution is a mixture of solid particles and gases in the air. Car emissions, chemicals from factories, ... Ozone, a gas, is a major part of air pollution in cities. When ozone forms air pollution, it's ...

  8. Integrating Internet Assignments into a Biochemistry/Molecular Biology Laboratory Course

    ERIC Educational Resources Information Center

    Kaspar, Roger L.

    2002-01-01

    A main challenge in educating undergraduate students is to introduce them to the Internet and to teach them how to effectively use it in research. To this end, an Internet assignment was developed that introduces students to websites related to biomedical research at the beginning of a biochemistry/molecular biology laboratory course. The basic…

  9. Sustaining Change in Upper Level Courses: Peer-Led Workshops in Organic Chemistry and Biochemistry

    ERIC Educational Resources Information Center

    Platt, Terry; Roth, Vicki; Kampmeier, Jack A.

    2008-01-01

    Our peer-led collaborative learning groups, called Workshops, have now had extended success in two upper-level courses in chemistry and biochemistry. These Workshops are in turn supported by a third upper-level course for training peer-leaders. Our data confirm that the initial positive results from the introduction of Workshops in organic…

  10. Cooperative Learning Combined with Short Periods of Lecturing: A Good Alternative in Teaching Biochemistry

    ERIC Educational Resources Information Center

    Fernandez-Santander, Ana

    2008-01-01

    The informal activities of cooperative learning and short periods of lecturing has been combined and used in the university teaching of biochemistry as part of the first year course of Optics and Optometry in the academic years 2004-2005 and 2005-2006. The lessons were previously elaborated by the teacher and included all that is necessary to…

  11. An SDS-PAGE Examination of Protein Quaternary Structure and Disulfide Bonding for a Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Powers, Jennifer L.; Andrews, Carla S.; St. Antoine, Caroline C.; Jain, Swapan S.; Bevilacqua, Vicky L. H.

    2005-01-01

    Electrophoresis is a valuable tool for biochemists, yet this technique is often not included in biochemistry laboratory curricula owing to time constraints or lack of equipment. Protein structure is also a topic of interest in many disciplines, yet most undergraduate lab experiments focus only on primary structure. In this experiment, students use…

  12. Using Adobe Flash Animations of Electron Transport Chain to Teach and Learn Biochemistry

    ERIC Educational Resources Information Center

    Teplá, Milada; Klímová, Helena

    2015-01-01

    Teaching the subject of the electron transport chain is one of the most challenging aspects of the chemistry curriculum at the high school level. This article presents an educational program called "Electron Transport Chain" which consists of 14 visual animations including a biochemistry quiz. The program was created in the Adobe Flash…

  13. EFFECTS OF ELEVATED CO2 AND TEMPERATURE ON GROWTH, BIOCHEMISTRY AND PHYSIOLOGY OF DOUGLAS-FIR

    EPA Science Inventory

    We examined the interactive effects of CO2 concentration and mean annual temperature on physiology, biochemistry and growth of Douglas fir seedlings. Seedlings were grown at ambient CO2 or ambient + 200 ppm CO2 and at ambient temperature or ambient + 4 ?C. Needle gas exchange m...

  14. A Proposal for Teaching Undergraduate Chemistry Students Carbohydrate Biochemistry by Problem-Based Learning Activities

    ERIC Educational Resources Information Center

    Figueira, Angela C. M.; Rocha, Joao B. T.

    2014-01-01

    This article presents a problem-based learning (PBL) approach to teaching elementary biochemistry to undergraduate students. The activity was based on "the foods we eat." It was used to engage students' curiosity and to initiate learning about a subject that could be used by the future teachers in the high school. The experimental…

  15. Impact of an In-Class Biochemistry Mini-Conference on Students' Perception of Science

    ERIC Educational Resources Information Center

    Gerczei, Timea

    2016-01-01

    The work presented here is the summary of a 3 year study that aimed to uncover how students' perception of science changes with the chance to participate in a mini-conference that is incorporated into the biochemistry lecture course. Students were asked to work in groups of 2 or 3 and research a topic that is related to the material covered in…

  16. Preparative Protein Production from Inclusion Bodies and Crystallization: A Seven-Week Biochemistry Sequence

    ERIC Educational Resources Information Center

    Peterson, Megan J.; Snyder, W. Kalani; Westerman, Shelley; McFarland, Benjamin J.

    2011-01-01

    We describe how to produce and purify proteins from "Escherichia coli" inclusion bodies by adapting versatile, preparative-scale techniques to the undergraduate laboratory schedule. This 7-week sequence of experiments fits into an annual cycle of research activity in biochemistry courses. Recombinant proteins are expressed as inclusion bodies,…

  17. Foundational Concepts and Underlying Theories for Majors in "Biochemistry and Molecular Biology"

    ERIC Educational Resources Information Center

    Tansey, John T.; Baird, Teaster, Jr.; Cox, Michael M.; Fox, Kristin M.; Knight, Jennifer; Sears, Duane; Bell, Ellis

    2013-01-01

    Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members and science educators from around the country that focused on identifying: 1) core principles of biochemistry and molecular biology, 2) essential concepts and underlying theories from physics, chemistry, and mathematics, and 3)…

  18. Web Camera Use in Developing Biology, Molecular Biology and Biochemistry Laboratories

    ERIC Educational Resources Information Center

    Ogren, Paul J.; Deibel, Michael; Kelly, Ian; Mulnix, Amy B.; Peck, Charlie

    2004-01-01

    The use of a network-ready color camera is described which is primarily marketed as a security device and is used for experiments in developmental biology, genetics and biochemistry laboratories and in special student research projects. Acquiring and analyzing project and archiving images is very important in microscopy, electrophoresis and…

  19. Implementing an Active Learning Environment to Influence Students' Motivation in Biochemistry

    ERIC Educational Resources Information Center

    Cicuto, Camila Aparecida Tolentino; Torres, Bayardo Baptista

    2016-01-01

    The Biochemistry: Biomolecules Structure and Metabolism course's goal is to promote meaningful learning through an active learning environment. Thus, study periods (SP) and discussion groups (DG) are used as a substitute for lecture classes. The goal of this study was to evaluate how this learning environment influences students' motivation (n =…

  20. Motivating Active Learning of Biochemistry through Artistic Representation of Scientific Concepts

    ERIC Educational Resources Information Center

    Bruna, Carola

    2013-01-01

    First-year students often feel discouraged, especially with courses that require complex thinking and involve establishing relations between different subjects such as biochemistry. It has been proposed that student-centred pedagogy can achieve motivation and improve learning. In this context, this case study reports the use of art as a strategy…

  1. Need Assessment of Enhancing the Weightage of Applied Biochemistry in the Undergraduate Curriculum at MGIMS, Sevagram

    ERIC Educational Resources Information Center

    Kumar, Satish; Jena, Lingaraja; Vagha, Jayant

    2016-01-01

    In order to review the need assessment of enhancing the weightage of Applied Biochemistry in the undergraduate curriculum at Mahatma Gandhi Institute of Medical Sciences (MGIMS), Sevagram, a validated questionnaire was sent to 453 participants which include 387 undergraduate students, 11 interns, 23 postgraduate students, and 32 faculty members. A…

  2. Essential Concepts and Underlying Theories from Physics, Chemistry, and Mathematics for "Biochemistry and Molecular Biology" Majors

    ERIC Educational Resources Information Center

    Wright, Ann; Provost, Joseph; Roecklein-Canfield, Jennifer A.; Bell, Ellis

    2013-01-01

    Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members from around the country. The workshops have focused on developing lists of Core Principles or Foundational Concepts in Biochemistry and Molecular Biology, a list of foundational skills, and foundational concepts from Physics, Chemistry,…

  3. A Semester-Long Project-Oriented Biochemistry Laboratory Based on "Helicobacter pylori" Urease

    ERIC Educational Resources Information Center

    Farnham, Kate R.; Dube, Danielle H.

    2015-01-01

    Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically…

  4. Known Structure, Unknown Function: An Inquiry-Based Undergraduate Biochemistry Laboratory Course

    ERIC Educational Resources Information Center

    Gray, Cynthia; Price, Carol W.; Lee, Christopher T.; Dewald, Alison H.; Cline, Matthew A.; McAnany, Charles E.; Columbus, Linda; Mura, Cameron

    2015-01-01

    Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry- and research-based could simultaneously impart scientific knowledge and foster a student's…

  5. The Concept Lens Diagram: A New Mechanism for Presenting Biochemistry Content in Terms of "Big Ideas"

    ERIC Educational Resources Information Center

    Rowland, Susan L.; Smith, Christopher A.; Gillam, Elizabeth M. A.; Wright, Tony

    2011-01-01

    A strong, recent movement in tertiary education is the development of conceptual, or "big idea" teaching. The emphasis in course design is now on promoting key understandings, core competencies, and an understanding of connections between different fields. In biochemistry teaching, this radical shift from the content-based tradition is being…

  6. Comprehensive Experiment--Clinical Biochemistry: Determination of Blood Glucose and Triglycerides in Normal and Diabetic Rats

    ERIC Educational Resources Information Center

    Jiao, Li; Xiujuan, Shi; Juan, Wang; Song, Jia; Lei, Xu; Guotong, Xu; Lixia, Lu

    2015-01-01

    For second year medical students, we redesigned an original laboratory experiment and developed a combined research-teaching clinical biochemistry experiment. Using an established diabetic rat model to detect blood glucose and triglycerides, the students participate in the entire experimental process, which is not normally experienced during a…

  7. Guided Inquiry in a Biochemistry Laboratory Course Improves Experimental Design Ability

    ERIC Educational Resources Information Center

    Goodey, Nina M.; Talgar, Cigdem P.

    2016-01-01

    Many biochemistry laboratory courses expose students to laboratory techniques through pre-determined experiments in which students follow stepwise protocols provided by the instructor. This approach fails to provide students with sufficient opportunities to practice experimental design and critical thinking. Ten inquiry modules were created for a…

  8. A Guide to Using Case-Based Learning in Biochemistry Education

    ERIC Educational Resources Information Center

    Kulak, Verena; Newton, Genevieve

    2014-01-01

    Studies indicate that the majority of students in undergraduate biochemistry take a surface approach to learning, associated with rote memorization of material, rather than a deep approach, which implies higher cognitive processing. This behavior relates to poorer outcomes, including impaired course performance and reduced knowledge retention. The…

  9. A Two-Week Guided Inquiry Protein Separation and Detection Experiment for Undergraduate Biochemistry

    ERIC Educational Resources Information Center

    Carolan, James P.; Nolta, Kathleen V.

    2016-01-01

    A laboratory experiment for teaching protein separation and detection in an undergraduate biochemistry laboratory course is described. This experiment, performed in two, 4 h laboratory periods, incorporates guided inquiry principles to introduce students to the concepts behind and difficulties of protein purification. After using size-exclusion…

  10. Integrating Bio-Inorganic and Analytical Chemistry into an Undergraduate Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Erasmus, Daniel J.; Brewer, Sharon E.; Cinel, Bruno

    2015-01-01

    Undergraduate laboratories expose students to a wide variety of topics and techniques in a limited amount of time. This can be a challenge and lead to less exposure to concepts and activities in bio-inorganic chemistry and analytical chemistry that are closely-related to biochemistry. To address this, we incorporated a new iron determination by…

  11. The Views of Undergraduates about Problem-Based Learning Applications in a Biochemistry Course

    ERIC Educational Resources Information Center

    Tarhan, Leman; Ayyildiz, Yildizay

    2015-01-01

    The effect of problem-based learning (PBL) applications in an undergraduate biochemistry course on students' interest in this course was investigated through four modules during one semester. Students' views about active learning and improvement in social skills were also collected and evaluated. We conducted the study with 36 senior students from…

  12. Teaching of Biochemistry in Medical School: A Well-Trodden Pathway?

    ERIC Educational Resources Information Center

    Mathews, Michael B.; Stagnaro-Green, Alex

    2008-01-01

    Biochemistry and molecular biology occupy a unique place in the medical school curriculum. They are frequently studied prior to medical school and are fundamental to the teaching of biomedical sciences in undergraduate medical education. These two circumstances, and the trend toward increased integration among the disciplines, have led to…

  13. A Statistical Analysis of College Biochemistry Textbooks in China: The Statuses on the Publishing and Usage

    ERIC Educational Resources Information Center

    Zhou, Ping; Wang, Qinwen; Yang, Jie; Li, Jingqiu; Guo, Junming; Gong, Zhaohui

    2015-01-01

    This study aimed to investigate the statuses on the publishing and usage of college biochemistry textbooks in China. A textbook database was constructed and the statistical analysis was adopted to evaluate the textbooks. The results showed that there were 945 (~57%) books for theory teaching, 379 (~23%) books for experiment teaching and 331 (~20%)…

  14. Filtrates & Residues: Hemoglobinometry--A Biochemistry Experiment that Utilizes the Principles of Transition Metal Chemistry.

    ERIC Educational Resources Information Center

    Giuliano, Vincenzo; Rieck, John Paul

    1987-01-01

    Describes a chemistry experiment dealing with hemoglobinometry that can apply to transition metal chemistry, colorimetry, and biochemistry. Provides a detailed description of the experimental procedure, including discussions of the preparation of the cyanide reagent, colorimetric measurements, and waste disposal and treatment. (TW)

  15. A Mechanism for Accelerating the Use of Non-Traditional Objectives and Methods in Biochemistry Courses.

    ERIC Educational Resources Information Center

    Mehler, Alan H.

    1991-01-01

    Suggests reasons for the lack of change in biochemistry courses and proposes mechanisms to enhance change. Advocates a problem-solving approach using nontraditional questions on examinations. Provides examples of how traditional questions can be rewritten to reflect a problem-solving approach. (PR)

  16. A Model of Factors Determining Students' Ability to Interpret External Representations in Biochemistry

    ERIC Educational Resources Information Center

    Schonborn, Konrad J.; Anderson, Trevor R.

    2009-01-01

    The aim of this research was to develop a model of factors affecting students' ability to interpret external representations (ERs) in biochemistry. The study was qualitative in design and was guided by the modelling framework of Justi and Gilbert. Application of the process outlined by the framework, and consultation with relevant literature, led…

  17. Introductory Course Based on a Single Problem: Learning Nucleic Acid Biochemistry from AIDS Research

    ERIC Educational Resources Information Center

    Grover, Neena

    2004-01-01

    In departure from the standard approach of using several problems to cover specific topics in a class, I use a single problem to cover the contents of the entire semester-equivalent biochemistry classes. I have developed a problem-based service-learning (PBSL) problem on HIV/AIDS to cover nucleic acid concepts that are typically taught in the…

  18. Virtual Office Hours Using a Tablet PC: E-lluminating Biochemistry in an Online Environment

    ERIC Educational Resources Information Center

    Kohorst, Kelly; Cox, James R.

    2007-01-01

    The availability of online collaboration software has provided new opportunities for instructors to interact with students outside the classroom. This report describes how Elluminate "Live!"[R], a particular conferencing software package, can be used with a tablet PC to conduct virtual office hours in a biochemistry course. The educational value…

  19. Using a Scholarly Approach to Improve Teaching and Learning in Biochemistry Higher Education

    ERIC Educational Resources Information Center

    Loertscher, Jennifer

    2012-01-01

    Although the biochemistry education community is far from avoiding conversations about improving teaching and learning, reexamining individual and community teaching practices through the lens of the scholarship of teaching and learning (SoTL) is critical for continued growth and improvement. The contemporary vision of SoTL, which has been…

  20. Locks and Keys: An Analysis of Biochemistry Students' Use of Analogies

    ERIC Educational Resources Information Center

    Orgill, MaryKay; Bodner, George

    2007-01-01

    Analogies can be powerful teaching tools because they can make abstract material intelligible to students by comparing it to material with which the students are already familiar. In this study, the authors interviewed 43 students to determine the specific ways they use analogies to learn and function in their biochemistry classes. They found that…

  1. A Learner-Centered Molecular Modeling Exercise for Allied Health Majors in a Biochemistry Class

    ERIC Educational Resources Information Center

    Fletcher, Terace M.; Ershler, Jeff

    2014-01-01

    Learner-centered molecular modeling exercises in college science courses can be especially challenging for nonchemistry majors as students typically have a higher degree of anxiety and may not appreciate the relevance of the work. This article describes a learner-centered project given to allied health majors in a Biochemistry course. The project…

  2. A Participatory Learning Approach to Biochemistry Using Student Authored and Evaluated Multiple-Choice Questions

    ERIC Educational Resources Information Center

    Bottomley, Steven; Denny, Paul

    2011-01-01

    A participatory learning approach, combined with both a traditional and a competitive assessment, was used to motivate students and promote a deep approach to learning biochemistry. Students were challenged to research, author, and explain their own multiple-choice questions (MCQs). They were also required to answer, evaluate, and discuss MCQs…

  3. Creating a Cell Map as an Active-Learning Tool in a Biochemistry Course

    ERIC Educational Resources Information Center

    Del Bianco, Cristina

    2010-01-01

    Teaching metabolism to a biochemistry class with diverse academic backgrounds is a challenging task. Often students lack the global perspective that is needed to understand how different metabolic pathways are reciprocally regulated. The classroom activity presented in this article is designed to facilitate the learning of metabolism by having the…

  4. Integrative Metabolism: An Interactive Learning Tool for Nutrition, Biochemistry, and Physiology

    ERIC Educational Resources Information Center

    Carey, Gale

    2010-01-01

    Metabolism is a dynamic, simultaneous, and integrative science that cuts across nutrition, biochemistry, and physiology. Teaching this science can be a challenge. The use of a scenario-based, visually appealing, interactive, computer-animated CD may overcome the limitations of learning "one pathway at a time" and engage two- and four-year college…

  5. The Metabolic Effects of Low-Carbohydrate Diets and Incorporation into a Biochemistry Course

    ERIC Educational Resources Information Center

    Pogozelski, Wendy; Arpaia, Nicholas; Priore, Salvatore

    2005-01-01

    One of the challenges in teaching biochemistry is facilitating students' interest in and mastery of metabolism. The many pathways and modes of regulation can be overwhelming for students to learn and difficult for professors to teach in an engaging manner. We have found it useful to take advantage of prevailing interest in popular yet…

  6. Use of Solid Phase Extraction in the Biochemistry Laboratory to Separate Different Lipids

    ERIC Educational Resources Information Center

    Flurkey, William H.

    2005-01-01

    Solid-phase extraction (SPE) was used to demonstrate how various lipids and lipid classes could be separated in a biochemistry laboratory setting. Three different SPE methods were chosen on their ability to separate a lipid mixture, consisting of a combination of a either a fatty acid, a triacylglycerol, a mono- or diacylglycerol, phospholipid,…

  7. The Kinetics and Inhibition of Gamma-Glutamyl Transpeptidase: A Biochemistry Laboratory Experiment.

    ERIC Educational Resources Information Center

    Splittgerber, A. G.; Sohl, Julie

    1988-01-01

    Discusses an enzyme kinetics laboratory experiment involving a two substrate system for undergraduate biochemistry. Uses the enzyme gamma-glutamyl transpeptidase as this enzyme in blood serum is of clinical significance. Notes elevated levels are seen in liver disease, alcoholism, and epilepsy. Uses a spectrophotometer for the analysis. (MVL)

  8. Forensic Analysis of Canine DNA Samples in the Undergraduate Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Carson, Tobin M.; Bradley, Sharonda Q.; Fekete, Brenda L.; Millard, Julie T.; LaRiviere, Frederick J.

    2009-01-01

    Recent advances in canine genomics have allowed the development of highly distinguishing methods of analysis for both nuclear and mitochondrial DNA. We describe a laboratory exercise suitable for an undergraduate biochemistry course in which the polymerase chain reaction is used to amplify hypervariable regions of DNA from dog hair and saliva…

  9. Establishment of Biochemistry Reference Values for Healthy Tanzanian Infants, Children, and Adolescents in Kilimanjaro Region

    PubMed Central

    Buchanan, Ann M.; Fiorillo, Suzanne P.; Omondi, Michael W.; Cunningham, Coleen K.; Crump, John A.

    2015-01-01

    Objective To establish common biochemistry reference intervals for Tanzanian infants, children, and adolescents living in the Kilimanjaro Region. Methods We recruited healthy, HIV-uninfected Tanzanian infants, children, and youth between the ages of one month and 17 years from local schools and clinics to participate in this study. Only afebrile children without signs of physical or chronic illness were enrolled. Nonparametric methods were used to determine 95% reference limits and their 90% confidence intervals, with outliers removed by the Tukey method. Results A total of 619 healthy infants, children, and adolescents were enrolled into the study. Twenty-three biochemistry parameters were measured. Compared to U.S. reference intervals, several of the biochemistry parameters showed notable differences; namely, alkaline phosphatase, phosphorus, amylase, and lipase. Comparing our data to the U.S. National Institutes of Health (NIH) Division of AIDS (DAIDS) grading criteria for classification of adverse events, we found that for select parameters, up to 15% of infants or children in certain age groups would have been categorized as having an adverse event as defined by DAIDS. Conclusions Our study further confirms the need to use locally established reference intervals to define reference laboratory parameters among children in Africa, rather than relying on those derived from U.S. or European populations. To our knowledge, this study provides the first set of locally validated biochemistry reference ranges for a pediatric population in Tanzania. PMID:26224122

  10. Development and Analysis of an Instrument to Assess Student Understanding of Foundational Concepts before Biochemistry Coursework

    ERIC Educational Resources Information Center

    Villafane, Sachel M.; Bailey, Cheryl P.; Loertscher, Jennifer; Minderhout, Vicky; Lewis, Jennifer E.

    2011-01-01

    Biochemistry is a challenging subject because student learning depends on the application of previously learned concepts from general chemistry and biology to new, biological contexts. This article describes the development of a multiple-choice instrument intended to measure five concepts from general chemistry and three from biology that are…

  11. Purification and Characterization of Taq Polymerase: A 9-Week Biochemistry Laboratory Project for Undergraduate Students

    ERIC Educational Resources Information Center

    Bellin, Robert M.; Bruno, Mary K.; Farrow, Melissa A.

    2010-01-01

    We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of "Thermus aquaticus" DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having each week's procedure focus on a single…

  12. Tagging and Purifying Proteins to Teach Molecular Biology and Advanced Biochemistry

    ERIC Educational Resources Information Center

    Roecklein-Canfield, Jennifer A.; Lopilato, Jane

    2004-01-01

    Two distinct courses, "Molecular Biology" taught by the Biology Department and "Advanced Biochemistry" taught by the Chemistry Department, complement each other and, when taught in a coordinated and integrated way, can enhance student learning and understanding of complex material. "Molecular Biology" is a comprehensive lecture-based course with a…

  13. Physical Models Enhance Molecular Three-Dimensional Literacy in an Introductory Biochemistry Course

    ERIC Educational Resources Information Center

    Roberts, Jacqueline R.; Hagedorn, Eric; Dillenburg, Paul; Patrick, Michael; Herman, Timothy

    2005-01-01

    This article reports the results of a recent study to evaluate the usefulness of physical models of molecular structures as a new tool with which to teach concepts of molecular structure and function. Of seven different learning tools used by students in this introductory biochemistry class, the use of the physical models in a laboratory was rated…

  14. Teaching foundational topics and scientific skills in biochemistry within the conceptual framework of HIV protease.

    PubMed

    Johnson, R Jeremy

    2014-01-01

    HIV protease has served as a model protein for understanding protein structure, enzyme kinetics, structure-based drug design, and protein evolution. Inhibitors of HIV protease are also an essential part of effective HIV/AIDS treatment and have provided great societal benefits. The broad applications for HIV protease and its inhibitors make it a perfect framework for integrating foundational topics in biochemistry around a big picture scientific and societal issue. Herein, I describe a series of classroom exercises that integrate foundational topics in biochemistry around the structure, biology, and therapeutic inhibition of HIV protease. These exercises center on foundational topics in biochemistry including thermodynamics, acid/base properties, protein structure, ligand binding, and enzymatic catalysis. The exercises also incorporate regular student practice of scientific skills including analysis of primary literature, evaluation of scientific data, and presentation of technical scientific arguments. Through the exercises, students also gain experience accessing computational biochemical resources such as the protein data bank, Proteopedia, and protein visualization software. As these HIV centered exercises cover foundational topics common to all first semester biochemistry courses, these exercises should appeal to a broad audience of undergraduate students and should be readily integrated into a variety of teaching styles and classroom sizes.

  15. Evaluation of a Modified Keller Method in a Biochemistry Laboratory Course.

    ERIC Educational Resources Information Center

    Jackman, Lance E.

    1982-01-01

    Describes a modified Keller plan for teaching a biochemistry laboratory course and evaluates the method's effectiveness by comparing results of students using Keller plan (N=17) to students receiving traditional instruction (N=16). Results suggest the modified Keller plan is a successful instructional method for the course. (Author/SK)

  16. Screen Capture on the Fly: Combining Molecular Visualization and a Tablet PC in the Biochemistry Lecture

    ERIC Educational Resources Information Center

    Cox, James R.

    2006-01-01

    The biochemistry lecture is often the place where students receive the greatest exposure to the structural nature of biomolecules. The use of molecular visualization software and web-based animated tutorials has enhanced the way instructors teach this important area of structural biology. Using the software and tutorials in class is an excellent…

  17. A guide to using case-based learning in biochemistry education.

    PubMed

    Kulak, Verena; Newton, Genevieve

    2014-01-01

    Studies indicate that the majority of students in undergraduate biochemistry take a surface approach to learning, associated with rote memorization of material, rather than a deep approach, which implies higher cognitive processing. This behavior relates to poorer outcomes, including impaired course performance and reduced knowledge retention. The use of case-based learning (CBL) into biochemistry teaching may facilitate deep learning by increasing student engagement and interest. Abundant literature on CBL exists but clear guidance on how to design and implement case studies is not readily available. This guide provides a representative review of CBL uses in science and describes the process of developing CBL modules to be used in biochemistry. Included is a framework to implement a directed CBL assisted with lectures in a content-driven biochemistry course regardless of class size. Moreover, this guide can facilitate adopting CBL to other courses. Consequently, the information presented herein will be of value to undergraduate science educators with an interest in active learning pedagogies.

  18. Improving Student Understanding of Lipids Concepts in a Biochemistry Course Using Test-Enhanced Learning

    ERIC Educational Resources Information Center

    Horn, Savannah; Hernick, Marcy

    2015-01-01

    Test-enhanced learning has successfully been used as a means to enhance learning and promote knowledge retention in students. We have examined whether this approach could be used in a biochemistry course to enhance student learning about lipids-related concepts. Students were provided access to two optional learning modules with questions related…

  19. Utilization of Electrochemical Sensors and Biosensors in Biochemistry and Molecular Biology

    PubMed Central

    Adam, Vojtech; Kizek, Rene

    2008-01-01

    A special issue of Sensors entitled “Utilization of Electrochemical Sensors and Biosensors in Biochemistry and Molecular Biology” has been prepared over a period of three years. In this Editorial Note we would like to highlight one of the possible directions for electrochemical sensor and biosensor research resulting from the ideas of Czechoslovakian Nobel Prize winner Jaroslav Heyrovsky and his colleague Rudolf Brdicka.

  20. Reversible Ligand Binding Reactions: Why Do Biochemistry Students Have Trouble Connecting the Dots?

    ERIC Educational Resources Information Center

    Sears, Duane W.; Thompson, Scott E.; Saxon, S. Robin

    2007-01-01

    Adaptive chemical behavior is essential for an organism's function and survival, and it is no surprise that biological systems are capable of responding both rapidly and selectively to chemical changes in the environment. To elucidate an organism's biochemistry, its chemical reactions need to be characterized in ways that reflect the normal…

  1. Students' Preferred Teaching Techniques for Biochemistry in Biomedicine and Medicine Courses

    ERIC Educational Resources Information Center

    Novelli, Ethel L.B.; Fernandes, Ana Angelica H.

    2007-01-01

    The aim of this study was to investigate the students' preferred teaching techniques, such as traditional blackboard, power-point, or slide-projection, for biochemistry discipline in biomedicine and medicine courses from Sao Paulo State University, UNESP, Botucatu, Sao Paulo, Brazil. Preferences for specific topic and teaching techniques were…

  2. Learning Medical School Biochemistry Through Self-Directed Case-Oriented Study.

    ERIC Educational Resources Information Center

    Morley, Colin G. D.; Blumberg, Phyllis

    1987-01-01

    Describes an alternative medical school curriculum for the first two years of preclinical basic science studies. Discusses student and faculty selection for the program. Details the format for teaching biochemistry in the Alternative Curriculum, including program structure, content organization and exams. Evaluates the success of the program. (CW)

  3. Kinetics of Carboxylesterase: An Experiment for Biochemistry and Physical Chemistry Laboratory.

    ERIC Educational Resources Information Center

    Nichols, C. S.; Cromartie, T. H.

    1979-01-01

    Describes a convenient, inexpensive experiment in enzyme kinetics developed for the undergraduate biochemistry laboratory at the University of Virginia. Required are a single beam visible spectrophotometer with output to a recorder, a constant temperature, a commercially available enzyme, substrates, and buffers. (BT)

  4. What Skills Should Students of Undergraduate Biochemistry and Molecular Biology Programs Have upon Graduation?

    ERIC Educational Resources Information Center

    White, Harold B.; Benore, Marilee A.; Sumter, Takita F.; Caldwell, Benjamin D.; Bell, Ellis

    2013-01-01

    Biochemistry and molecular biology (BMB) students should demonstrate proficiency in the foundational concepts of the discipline and possess the skills needed to practice as professionals. To ascertain the skills that should be required, groups of BMB educators met in several focused workshops to discuss the expectations with the ultimate goal of…

  5. Thermodynamic Exploration of Eosin-Lysozyme Binding: A Physical Chemistry and Biochemistry Laboratory Experiment

    ERIC Educational Resources Information Center

    Huisman, Andrew J.; Hartsell, Lydia R.; Krueger, Brent P.; Pikaart, Michael J.

    2010-01-01

    We developed a modular pair of experiments for use in the undergraduate physical chemistry and biochemistry laboratories. Both experiments examine the thermodynamics of the binding of a small molecule, eosin Y, to the protein lysozyme. The assay for binding is the quenching of lysozyme fluorescence by eosin through resonant energy transfer. In…

  6. Teaching Protein Purification and Characterization Techniques: A Student-Initiated, Project-Oriented Biochemistry Laboratory Course

    ERIC Educational Resources Information Center

    MacDonald, Gina

    2008-01-01

    This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, and made buffers they continue to learn basic protein purification and biochemical techniques…

  7. Use of Molecular Models for Active Learning in Biochemistry Lecture Courses

    ERIC Educational Resources Information Center

    Hageman, James H.

    2010-01-01

    The pedagogical value of having biochemistry and organic chemistry students build and manipulate physical models of chemical species is well established in the literature. Nevertheless, for the most part, the use of molecular models is generally limited to several laboratory exercises or to demonstrations in the classroom setting. A simple…

  8. A Pilot Project in Augmentative Distance Learning... George Mason University Graduate Course in Biochemistry.

    ERIC Educational Resources Information Center

    Willett, James D.; Keefe, David D.

    This paper describes the use of distance learning capabilities to augment and amplify the learning opportunities for part-time graduate students at George Mason University. The students in the biochemistry course described meet periodically on campus for brief interactions with the instructor and peer classmates. Between these synchronous…

  9. A Course Designed for Undergraduate Biochemistry Students to Learn about Cultural Diversity Issues

    ERIC Educational Resources Information Center

    Benore-Parsons, Marilee

    2006-01-01

    Biology, biochemistry, and other science students are well trained in science and familiar with how to conduct and evaluate scientific experiments. They are less aware of cultural issues or how these will impact their careers in research, education, or as professional health care workers. A course was developed for advanced undergraduate science…

  10. Hematology and plasma biochemistry values of great cormorant (Phalacrocorax carbo sinensis) nestlings.

    PubMed

    Minias, Piotr; Kaczmarek, Krzysztof; Janiszewski, Tomasz; Markowski, Janusz

    2013-01-01

    Hematology and plasma biochemistry values were determined in 92 free-living Great Cormorant (Phalacrocorax carbo sinensis) chicks at Jeziorsko reservoir, central Poland. Percentage distribution of leukocytes, packed cell volume, plasma concentrations of hemoglobin and basic biochemical parameters were evaluated. These values may be treated as reference ranges for free-living Great Cormorant nestlings. PMID:23307389

  11. Reference intervals for selected serum biochemistry analytes in cheetahs Acinonyx jubatus.

    PubMed

    Hudson-Lamb, Gavin C; Schoeman, Johan P; Hooijberg, Emma H; Heinrich, Sonja K; Tordiffe, Adrian S W

    2016-01-01

    Published haematologic and serum biochemistry reference intervals are very scarce for captive cheetahs and even more for free-ranging cheetahs. The current study was performed to establish reference intervals for selected serum biochemistry analytes in cheetahs. Baseline serum biochemistry analytes were analysed from 66 healthy Namibian cheetahs. Samples were collected from 30 captive cheetahs at the AfriCat Foundation and 36 free-ranging cheetahs from central Namibia. The effects of captivity-status, age, sex and haemolysis score on the tested serum analytes were investigated. The biochemistry analytes that were measured were sodium, potassium, magnesium, chloride, urea and creatinine. The 90% confidence interval of the reference limits was obtained using the non-parametric bootstrap method. Reference intervals were preferentially determined by the non-parametric method and were as follows: sodium (128 mmol/L - 166 mmol/L), potassium (3.9 mmol/L - 5.2 mmol/L), magnesium (0.8 mmol/L - 1.2 mmol/L), chloride (97 mmol/L - 130 mmol/L), urea (8.2 mmol/L - 25.1 mmol/L) and creatinine (88 µmol/L - 288 µmol/L). Reference intervals from the current study were compared with International Species Information System values for cheetahs and found to be narrower. Moreover, age, sex and haemolysis score had no significant effect on the serum analytes in this study. Separate reference intervals for captive and free-ranging cheetahs were also determined. Captive cheetahs had higher urea values, most likely due to dietary factors. This study is the first to establish reference intervals for serum biochemistry analytes in cheetahs according to international guidelines. These results can be used for future health and disease assessments in both captive and free-ranging cheetahs. PMID:26974036

  12. Enhancing Predictive Accuracy of Cardiac Autonomic Neuropathy Using Blood Biochemistry Features and Iterative Multitier Ensembles.

    PubMed

    Abawajy, Jemal; Kelarev, Andrei; Chowdhury, Morshed U; Jelinek, Herbert F

    2016-01-01

    Blood biochemistry attributes form an important class of tests, routinely collected several times per year for many patients with diabetes. The objective of this study is to investigate the role of blood biochemistry for improving the predictive accuracy of the diagnosis of cardiac autonomic neuropathy (CAN) progression. Blood biochemistry contributes to CAN, and so it is a causative factor that can provide additional power for the diagnosis of CAN especially in the absence of a complete set of Ewing tests. We introduce automated iterative multitier ensembles (AIME) and investigate their performance in comparison to base classifiers and standard ensemble classifiers for blood biochemistry attributes. AIME incorporate diverse ensembles into several tiers simultaneously and combine them into one automatically generated integrated system so that one ensemble acts as an integral part of another ensemble. We carried out extensive experimental analysis using large datasets from the diabetes screening research initiative (DiScRi) project. The results of our experiments show that several blood biochemistry attributes can be used to supplement the Ewing battery for the detection of CAN in situations where one or more of the Ewing tests cannot be completed because of the individual difficulties faced by each patient in performing the tests. The results show that AIME provide higher accuracy as a multitier CAN classification paradigm. The best predictive accuracy of 99.57% has been obtained by the AIME combining decorate on top tier with bagging on middle tier based on random forest. Practitioners can use these findings to increase the accuracy of CAN diagnosis.

  13. Reference intervals for selected serum biochemistry analytes in cheetahs Acinonyx jubatus.

    PubMed

    Hudson-Lamb, Gavin C; Schoeman, Johan P; Hooijberg, Emma H; Heinrich, Sonja K; Tordiffe, Adrian S W

    2016-02-26

    Published haematologic and serum biochemistry reference intervals are very scarce for captive cheetahs and even more for free-ranging cheetahs. The current study was performed to establish reference intervals for selected serum biochemistry analytes in cheetahs. Baseline serum biochemistry analytes were analysed from 66 healthy Namibian cheetahs. Samples were collected from 30 captive cheetahs at the AfriCat Foundation and 36 free-ranging cheetahs from central Namibia. The effects of captivity-status, age, sex and haemolysis score on the tested serum analytes were investigated. The biochemistry analytes that were measured were sodium, potassium, magnesium, chloride, urea and creatinine. The 90% confidence interval of the reference limits was obtained using the non-parametric bootstrap method. Reference intervals were preferentially determined by the non-parametric method and were as follows: sodium (128 mmol/L - 166 mmol/L), potassium (3.9 mmol/L - 5.2 mmol/L), magnesium (0.8 mmol/L - 1.2 mmol/L), chloride (97 mmol/L - 130 mmol/L), urea (8.2 mmol/L - 25.1 mmol/L) and creatinine (88 µmol/L - 288 µmol/L). Reference intervals from the current study were compared with International Species Information System values for cheetahs and found to be narrower. Moreover, age, sex and haemolysis score had no significant effect on the serum analytes in this study. Separate reference intervals for captive and free-ranging cheetahs were also determined. Captive cheetahs had higher urea values, most likely due to dietary factors. This study is the first to establish reference intervals for serum biochemistry analytes in cheetahs according to international guidelines. These results can be used for future health and disease assessments in both captive and free-ranging cheetahs.

  14. Correlation of Preadmission Organic Chemistry Courses and Academic Performance in Biochemistry at a Midwest Chiropractic Doctoral Program*

    PubMed Central

    McRae, Marc P.

    2010-01-01

    Purpose: Organic chemistry has been shown to correlate with academic success in the preclinical years of medicine, dentistry, and graduate physiology. The purpose of this study is to examine the relationship between undergraduate organic chemistry grades and first-semester biochemistry grades at a Midwest chiropractic doctoral program. Methods: Students enrolled in a first-semester biochemistry course who had completed the prerequisite courses in organic chemistry offered at this same institution were entered into the study. The total grade for each of the three courses was calculated using the midterm and final exam raw scores with a weighting of 50% each. Analysis consisted of obtaining correlation coefficients between the total grades of organic 1 with biochemistry and organic 2 with biochemistry. Using the biochemistry total grade, the students were divided into quartiles and course grades for both organic chemistry 1 and 2 were calculated. Results: For the 109 students in the study, the correlation coefficient between the biochemistry and organic chemistry 1 and biochemistry and organic chemistry 2 courses was r = 0.744 and r = 0.725, respectively. The difference in organic chemistry grades between those in the first and fourth quartiles was 63.2% and 86.9% for organic chemistry 1 (p < .001) and 60.9% and 79.4% for organic chemistry 2 (p < .001). Conclusion: This study shows that organic chemistry can be used as an indicator of future academic success in a chiropractic biochemistry course. Knowledge of such a relationship could prove useful to identify students who may potentially run into academic difficulty with first-year biochemistry PMID:20480012

  15. Design of a comprehensive biochemistry and molecular biology experiment: phase variation caused by recombinational regulation of bacterial gene expression.

    PubMed

    Sheng, Xiumei; Xu, Shungao; Lu, Renyun; Isaac, Dadzie; Zhang, Xueyi; Zhang, Haifang; Wang, Huifang; Qiao, Zheng; Huang, Xinxiang

    2014-01-01

    Scientific experiments are indispensable parts of Biochemistry and Molecular Biology. In this study, a comprehensive Biochemistry and Molecular Biology experiment about Salmonella enterica serovar Typhi Flagellar phase variation has been designed. It consisted of three parts, namely, inducement of bacterial Flagellar phase variation, antibody agglutination test, and PCR analysis. Phase variation was observed by baterial motility assay and identified by antibody agglutination test and PCR analysis. This comprehensive experiment can be performed to help students improve their ability to use the knowledge acquired in Biochemistry and Molecular Biology.

  16. Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina.

    PubMed

    Rinland, María Emilia; Gómez, Marisa Anahí

    2015-03-01

    Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes. PMID:25586510

  17. The type specimens of Tachinidae (Diptera) housed in the Museo Argentino de Ciencias Naturales "Bernardino Rivadavia", Buenos Aires.

    PubMed

    Mulieri, Pablo Ricardo; Patitucci, Luciano Damián; Bachmann, Axel Oscar; O'Hara, James E

    2013-01-01

    The type material of species of Tachinidae (Diptera) housed in the collection of the Entomology Division of the Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" were examined and are herein documented. The collection contains 202 type specimens consisting of 54 species described by E.E. Blanchard and 12 described by J. Brèthes. Comparison of their original descriptions with the label information reveals the existence of 24 holotypes, 1 lectotype, 141 syntypes and 36 paratypes. Complete information is given for each type, including reference to the original description, label data, and preservation condition.

  18. [Biocultural study of infant malnutrition of primary cause in the urban setting of La Plata, Buenos Aires Province, Argentina].

    PubMed

    Ortale, S; Rodrigo, M A

    1996-06-01

    The present study, of microsocial nature, attempts to recover the biology-culture unity in the analysis of child undernutrition. A sample was selected of 52 children with primary undernutrition and their families, from the population attending the Nutritional Rehabilitation Unit of La Plata Children Hospital. The specific index was used to measure the low socioeconomic level in families with undernourished children. The mothers were interviewed to obtain information on the perception and recognition of undernutrition. Observation and interviews to the medical staff were performed in ten centers of primary health care (PHC), in order to reveal the medical practices related to this problem. The results obtained showed that the studied families conjugated the most unfavorable life conditions within poverty. Children with adequate weight at birth were nutritionally affected between 3 and 18 months of age, leading to height disorders. The representations and practices sustained by the mothers, relating undernutrition to a popular disease named "pata de cabra" lead to limitations in treatment. In PHC centers, conceptual and operative limitations are recorded for its detection and early treatment. It is concluded that cultural factors present in the two most directly related agents to child health (the mother and the PHC center) do not allow to modify the environmental effects that prematurely affect the children of poverty.

  19. Generalized cost-effectiveness analysis of a package of interventions to reduce cardiovascular disease in Buenos Aires, Argentina

    PubMed Central

    Rubinstein, Adolfo; García Martí, Sebastián; Souto, Alberto; Ferrante, Daniel; Augustovski, Federico

    2009-01-01

    Background Chronic diseases, represented mainly by cardiovascular disease (CVD) and cancer, are increasing in developing countries and account for 53% of chronic diseases in Argentina. There is strong evidence that a reduction of 50% of the deaths due to CVD can be attributed to a reduction in smoking, hypertension and hypercholesterolemia. Generalized cost-effectiveness analysis (GCE) is a methodology designed by WHO to inform decision makers about the extent to which current or new interventions represent an efficient use of resources. We aimed to use GCE analysis to identify the most efficient interventions to decrease CVD. Methods Six individual interventions (treatment of hypertension, hypercholesterolemia, smoking cessation and combined clinical strategies to reduce the 10 year CVD Risk) and two population-based interventions (cooperation between government, consumer associations and bakery chambers to reduce salt in bread, and mass education strategies to reduce hypertension, hypercholesterolemia and obesity) were selected for analysis. Estimates of effectiveness were entered into age and sex specific models to predict their impact in terms of age-weighted and discounted DALYs saved (disability-adjusted life years). To translate the age- and sex-adjusted incidence of CVD events into health changes, we used risk model software developed by WHO (PopMod). Costs of services were measured in Argentine pesos, and discounted at an annual rate of 3%. Different budgetary impact scenarios were explored. Results The average cost-effectiveness ratio in argentine pesos (ARS$) per DALY for the different interventions were: (i) less salt in bread $151; (ii) mass media campaign $547; (iii) combination drug therapy provided to subjects with a 20%, 10% and 5% global CVD risk, $3,599, $4,113 and $4,533, respectively; (iv) high blood pressure (HBP) lowering therapy $7,716; (v) tobacco cessation with bupropion $ 33,563; and (iv) high-cholesterol lowering therapy with statins $ 70,994. Conclusion Against a threshold of average per capita income in Argentina, the two selected population-based interventions (lowering salt intake and health education through mass-media campaigns) plus the modified polypill strategy targeting people with a 20% or greater risk were cost-effective. Use of this methodology in developing countries can make resource-allocation decisions less intuitive and more driven by evidence. PMID:19419570

  20. Insecticide toxicity to Hyalella curvispina in runoff and stream water within a soybean farm (Buenos Aires, Argentina).

    PubMed

    Mugni, H; Ronco, A; Bonetto, C

    2011-03-01

    Toxicity to the locally dominant amphipod Hyalella curvispina was assessed in a first-order stream running through a cultivated farm. Cypermethrin, chlorpyrifos, endosulfan and glyphosate were sprayed throughout the studied period. Toxicity was assayed under controlled laboratory conditions with runoff and stream water samples taken from the field under steady state and flood conditions. Ephemeral toxicity pulses were observed as a consequence of farm pesticide applications. After pesticide application, runoff water showed 100% mortality to H. curvispina for 1 month, but no mortality thereafter. Toxicity persistence was shortest in stream water, intermediate in stream sediments and longest in soil samples. Runoff had a more important toxicity effect than the exposure to direct aerial fumigation. The regional environmental features determining fast toxicity dissipation are discussed.