Noninvasive Biomonitoring Approaches to Determine Dosimetry and Risk Following Acute Chemical Exposure: Analysis of Lead or Organophosphate Insecticide in Saliva

DOE Office of Scientific and Technical Information (OSTI.GOV)

Timchalk, Chuck; Poet, Torka S.; Kousba, Ahmed A.

2004-04-01

There is a need to develop approaches for assessing risk associated with acute exposures to a broad-range of chemical agents and to rapidly determine the potential implications to human health. Non-invasive biomonitoring approaches are being developed using reliable portable analytical systems to quantitate dosimetry utilizing readily obtainable body fluids, such as saliva. Saliva has been used to evaluate a broad range of biomarkers, drugs, and environmental contaminants including heavy metals and pesticides. To advance the application of non-invasive biomonitoring a microfluidic/ electrochemical device has also been developed for the analysis of lead (Pb), using square wave anodic stripping voltammetry. Themore » system demonstrates a linear response over a broad concentration range (1 2000 ppb) and is capable of quantitating saliva Pb in rats orally administered acute doses of Pb-acetate. Appropriate pharmacokinetic analyses have been used to quantitate systemic dosimetry based on determination of saliva Pb concentrations. In addition, saliva has recently been used to quantitate dosimetry following exposure to the organophosphate insecticide chlorpyrifos in a rodent model system by measuring the major metabolite, trichloropyridinol, and saliva cholinesterase inhibition following acute exposures. These results suggest that technology developed for non-invasive biomonitoring can provide a sensitive, and portable analytical tool capable of assessing exposure and risk in real-time. By coupling these non-invasive technologies with pharmacokinetic modeling it is feasible to rapidly quantitate acute exposure to a broad range of chemical agents. In summary, it is envisioned that once fully developed, these monitoring and modeling approaches will be useful for accessing acute exposure and health risk.« less

CONCLUSIONS AND RECOMMENDATIONS OF THE EXPERT PANEL: TECHNICAL WORKSHOP ON HUMAN MILK SURVEILLANCE AND BIOMONITORING FOR ENVIRONMENTAL CHEMICALS IN THE UNITED STATES

EPA Science Inventory

The Technical Workshop focused on questions related to interpretation of information gathered from human milk biomonitoring studies. Biomonitoring can measure a person’s exposure to a chemical in his/her tissue. Human milk is a unique biological matrix for biomonitoring because i...

The Use of Biomonitoring Data in Exposure and Human Health Risk Assessments

PubMed Central

Albertini, Richard; Bird, Michael; Doerrer, Nancy; Needham, Larry; Robison, Steven; Sheldon, Linda; Zenick, Harold

2006-01-01

Biomonitoring uses analytic methods that permit the accurate measurement of low levels of environmental chemicals in human tissues. However, depending on the intended use, biomonitoring, like all exposure tools, may not be a stand-alone exposure assessment tool for some of its environmental public health uses. Although biomonitoring data demonstrate that many environmental chemicals are absorbed in human tissues, uncertainty exists regarding if and at what concentrations many of these chemicals cause adverse health outcomes. Moreover, without exposure pathway information, it is difficult to relate biomonitoring results to sources and routes of exposure and develop effective health risk management strategies. In September 2004, the Health and Environmental Sciences Institute, U.S. Environmental Protection Agency, Centers for Disease Control and Prevention, Agency for Toxic Substances and Disease Registry, and International Council of Chemical Associations co-sponsored the International Biomonitoring Workshop, which explored the processes and information needed for placing biomonitoring data into perspective for risk assessment purposes, with special emphasis on integrating biomarker measurements of exposure, internal dose, and potential health outcome. Scientists from international governments, academia, and industry recommended criteria for applying biomonitoring data for various uses. Six case studies, which are part of this mini-monograph, were examined: inorganic arsenic, methyl eugenol, organophosphorus pesticides, perfluorooctanesulfonate, phthalates, and polybrominated diphenyl ethers. Based on the workshop and follow-up discussions, this overview article summarizes lessons learned, identifies data gaps, outlines research needs, and offers guidance for designing and conducting biomonitoring studies, as well as interpreting biomonitoring data in the context of risk assessment and risk management. PMID:17107864

Laser and biological methods of biomonitoring of surrounding waters

NASA Astrophysics Data System (ADS)

Posudin, Yuri I.

1994-02-01

Three main methods are proposed for the biomonitoring of chemicals in water medium: laser spectrofluorometry, which is based on the excitation and recording of the spectra of fluorescence; laser scattering, which is connected with measurement of the Doppler shifts of the scattered light from the motile cells; videomicrography, which provides the analysis of parameters of photomovement of motile cells via microscope and video system. Such chemicals as surface-active substances, heavy metals and pesticides were determined in water medium due to these methods.

Morbidity study of extruder personnel with potential exposure to brominated dioxins and furans. I. Results of blood monitoring and immunological tests.

PubMed Central

Zober, M A; Ott, M G; Päpke, O; Senft, K; Germann, C

1992-01-01

The potential for exposure of employees to polybrominated dibenzofurans (PBDFs) and dibenzo-p-dioxins (PBDDs) during extrusion blending of resins containing decabromodiphenyl ether was established through previous air monitoring (area samples) and biomonitoring studies. The findings presented herein are further biomonitoring results for 42 employees and immunological tests for exposed and referent employees. Among potentially exposed men, 2,3,7,8-TBDF and 2,3,7,8-TBDD concentrations in blood lipid ranged from non-detectable to 112 parts per trillion (ppt) and from non-detectable to 478 ppt respectively. Biomonitoring results correlated well with assignments in the extruder work area when adjusted for process changes and engineering improvements and provided biological half life estimates of between 1.1 and 1.9 years for 2,3,7,8-TBDF and between 2.9 and 10.8 years for 2,3,7,8-TBDD. Results for 16 measures of the immune system were examined in relation to exposure (exposed v referent group) and in relation to the biomonitoring data. Some individual trends in immunological parameters with exposure and covariates such as age and cigarette smoking were found (for example, an increase in complement C4 with increasing concentrations of PBDFs and PBDDs, increased lymphocyte subpopulation counts with cigarette smoking); however, the overall clinical assessment was that the immune system of exposed employees was not adversely impacted at these burdens of PBDFs and PBDDs. PMID:1515345

Morbidity study of extruder personnel with potential exposure to brominated dioxins and furans. I. Results of blood monitoring and immunological tests.

PubMed

Zober, M A; Ott, M G; Päpke, O; Senft, K; Germann, C

1992-08-01

The potential for exposure of employees to polybrominated dibenzofurans (PBDFs) and dibenzo-p-dioxins (PBDDs) during extrusion blending of resins containing decabromodiphenyl ether was established through previous air monitoring (area samples) and biomonitoring studies. The findings presented herein are further biomonitoring results for 42 employees and immunological tests for exposed and referent employees. Among potentially exposed men, 2,3,7,8-TBDF and 2,3,7,8-TBDD concentrations in blood lipid ranged from non-detectable to 112 parts per trillion (ppt) and from non-detectable to 478 ppt respectively. Biomonitoring results correlated well with assignments in the extruder work area when adjusted for process changes and engineering improvements and provided biological half life estimates of between 1.1 and 1.9 years for 2,3,7,8-TBDF and between 2.9 and 10.8 years for 2,3,7,8-TBDD. Results for 16 measures of the immune system were examined in relation to exposure (exposed v referent group) and in relation to the biomonitoring data. Some individual trends in immunological parameters with exposure and covariates such as age and cigarette smoking were found (for example, an increase in complement C4 with increasing concentrations of PBDFs and PBDDs, increased lymphocyte subpopulation counts with cigarette smoking); however, the overall clinical assessment was that the immune system of exposed employees was not adversely impacted at these burdens of PBDFs and PBDDs.

MEASURING CHOLINESTERASE ACTIVITY IN HUMAN STUDIES.

EPA Science Inventory

Biomonitoring of organophosphorous and carbamate pesticides has focused primarily on the inhibition of blood cholinesterase. Blood biomonitoring, however, can be invasive, time-consuming, and costly, especially in young children and infants. Therefore, saliva biomonitoring ha...

Advancing environmental health surveillance in the US through a national human biomonitoring network.

PubMed

Latshaw, Megan Weil; Degeberg, Ruhiyyih; Patel, Surili Sutaria; Rhodes, Blaine; King, Ewa; Chaudhuri, Sanwat; Nassif, Julianne

2017-03-01

The United States lacks a comprehensive, nationally-coordinated, state-based environmental health surveillance system. This lack of infrastructure leads to: • varying levels of understanding of chemical exposures at the state & local levels • often inefficient public health responses to chemical exposure emergencies (such as those that occurred in the Flint drinking water crisis, the Gold King mine spill, the Elk river spill and the Gulf Coast oil spill) • reduced ability to measure the impact of public health interventions or environmental policies • less efficient use of resources for cleaning up environmental contamination Establishing the National Biomonitoring Network serves as a step toward building a national, state-based environmental health surveillance system. The Network builds upon CDC investments in emergency preparedness and environmental public health tracking, which have created advanced chemical analysis and information sharing capabilities in the state public health systems. The short-term goal of the network is to harmonize approaches to human biomonitoring in the US, thus increasing the comparability of human biomonitoring data across states and communities. The long-term goal is to compile baseline data on exposures at the state level, similar to data found in CDC's National Report on Human Exposure to Environmental Chemicals. Barriers to success for this network include: available resources, effective risk communication strategies, data comparability & sharing, and political will. Anticipated benefits include high quality data on which to base public health and environmental decisions, data with which to assess the success of public health interventions, improved risk assessments for chemicals, and new ways to prioritize environmental health research. Copyright © 2016 Elsevier GmbH. All rights reserved.

BIOMONITORING OF EXPOSURE IN FARMWORKER STUDIES

EPA Science Inventory

Though biomonitoring has been used in many occupational and environmental health and exposure studies, we are only beginning to understand the complexities and uncertainties involved with the biomonitoring process -- from study design, to sample collection, to chemical analysis -...

A new spin on research translation: the Boston Consensus Conference on Human Biomonitoring.

PubMed

Nelson, Jessica W; Scammell, Madeleine Kangsen; Altman, Rebecca Gasior; Webster, Thomas F; Ozonoff, David M

2009-04-01

Translating research to make it more understandable and effective (research translation) has been declared a priority in environmental health but does not always include communication to the public or residents of communities affected by environmental hazards. Their unique perspectives are also commonly missing from discussions about science and technology policy. The consensus conference process, developed in Denmark, offers a way to address this gap. The Boston Consensus Conference on Human Biomonitoring, held in Boston, Massachusetts, in the fall of 2006, was designed to educate and elicit input from 15 Boston-area residents on the scientifically complex topic of human biomonitoring for environmental chemicals. This lay panel considered the many ethical, legal, and scientific issues surrounding biomonitoring and prepared a report expressing their views. The lay panel's findings provide a distinct and important voice on the expanding use of biomonitoring. In some cases, such as a call for opt-in reporting of biomonitoring results to study participants, they mirror recommendations raised elsewhere. Other conclusions have not been heard previously, including the recommendation that an individual's results should be statutorily exempted from the medical record unless permission is granted, and the opportunity to use biomonitoring data to stimulate green chemistry. The consensus conference model addresses both aspects of a broader conception of research translation: engaging the public in scientific questions, and bringing their unique perspectives to bear on public health research, practice, and policy. In this specific application, a lay panel's recommendations on biomonitoring surveillance, communication, and ethics have practical implications for the conduct of biomonitoring studies and surveillance programs.

INTEGRATION OF BIOMONITORING EXPOSURE DATA INTO THE RISK ASSESSMENT PROCESS

EPA Science Inventory

Improved biomonitoring techniques are being used to measure very low levels of environmental chemicals in the tissues of adults and children. Public and private demand for biomonitoring data are on the increase worldwide. In the United States alone, government-sponsored programs...

Evaluation of Biomonitoring Data from the CDC National Exposure Report in a Risk Assessment Context: Perspectives across Chemicals

EPA Science Inventory

BACKGROUND: Biomonitoring data reported in the National Report on Human Exposure to Environmental Chemicals (NER) provide information on the presence and concentrations of more than 400 chemicals in human blood and urine. Biomonitoring Equivalents (BEs) and other risk assessment...

MAKING SENSE OF HUMAN BIOMONITORING DATA: FINDINGS AND RECOMMENDATIONS OF A WORKSHOP

EPA Science Inventory

The ability to measure chemicals in humans (often termed biomonitoring) is far outpacing the ability to reliably interpret these data for public health purposes, creating a major knowledge gap. Until this gap is filled, the great promise of routinely using biomonitoring data to s...

Human body burdens of chemicals used in plastic manufacture

PubMed Central

Koch, Holger M.; Calafat, Antonia M.

2009-01-01

In the last decades, the availability of sophisticated analytical chemistry techniques has facilitated measuring trace levels of multiple environmental chemicals in human biological matrices (i.e. biomonitoring) with a high degree of accuracy and precision. As biomonitoring data have become readily available, interest in their interpretation has increased. We present an overview on the use of biomonitoring in exposure and risk assessment using phthalates and bisphenol A as examples of chemicals used in the manufacture of plastic goods. We present and review the most relevant research on biomarkers of exposure for phthalates and bisphenol A, including novel and most comprehensive biomonitoring data from Germany and the United States. We discuss several factors relevant for interpreting and understanding biomonitoring data, including selection of both biomarkers of exposure and human matrices, and toxicokinetic information. PMID:19528056

Environmental Barcoding: A Next-Generation Sequencing Approach for Biomonitoring Applications Using River Benthos

PubMed Central

Hajibabaei, Mehrdad; Shokralla, Shadi; Zhou, Xin; Singer, Gregory A. C.; Baird, Donald J.

2011-01-01

Timely and accurate biodiversity analysis poses an ongoing challenge for the success of biomonitoring programs. Morphology-based identification of bioindicator taxa is time consuming, and rarely supports species-level resolution especially for immature life stages. Much work has been done in the past decade to develop alternative approaches for biodiversity analysis using DNA sequence-based approaches such as molecular phylogenetics and DNA barcoding. On-going assembly of DNA barcode reference libraries will provide the basis for a DNA-based identification system. The use of recently introduced next-generation sequencing (NGS) approaches in biodiversity science has the potential to further extend the application of DNA information for routine biomonitoring applications to an unprecedented scale. Here we demonstrate the feasibility of using 454 massively parallel pyrosequencing for species-level analysis of freshwater benthic macroinvertebrate taxa commonly used for biomonitoring. We designed our experiments in order to directly compare morphology-based, Sanger sequencing DNA barcoding, and next-generation environmental barcoding approaches. Our results show the ability of 454 pyrosequencing of mini-barcodes to accurately identify all species with more than 1% abundance in the pooled mixture. Although the approach failed to identify 6 rare species in the mixture, the presence of sequences from 9 species that were not represented by individuals in the mixture provides evidence that DNA based analysis may yet provide a valuable approach in finding rare species in bulk environmental samples. We further demonstrate the application of the environmental barcoding approach by comparing benthic macroinvertebrates from an urban region to those obtained from a conservation area. Although considerable effort will be required to robustly optimize NGS tools to identify species from bulk environmental samples, our results indicate the potential of an environmental barcoding approach for biomonitoring programs. PMID:21533287

Usage Proposal of a common urban decorative tree (Salix alba L.) to monitor the dispersion of gaseous mercury: A case study from Turda (Romania).

PubMed

Esbrí, J M; Cacovean, H; Higueras, P

2018-02-01

Closure of chloralkali plants poses a risk of abandonment of important sources of gaseous mercury. In this work, an assessment has been made of the potential for pollution from one of these plants in the proximity of a densely populated town in central Romania. The work involved a comparison between two major types of monitoring survey: biomonitoring using leaves of a tree common in urban environments; and LUMEX-based gaseous mercury analysis. For biomonitoring, 21 samples from Salix alba L. trees were taken in Turda area. Atmospheric monitoring included two mobile surveys and one at a fixed location. The results from both monitoring systems show similarities in gaseous mercury dispersion patterns, with high mercury contents clearly related to the presence of the chloralkali plant. Particularly high levels were measured in the following situations: (i) in a 'smog' area related with thermal inversion and (ii) during dusk. Direct monitoring suffered from limitations in acquiring information, especially in a medium-long time range, but biomonitoring provided these data and is capable of covering studies on temporary trends or comparative assessments between European cities with contrasting gaseous mercury sources. The thermal speciation of mercury contents indicates that the whole fraction of mercury in leaves corresponds to organic mercury. This finding implies a non-reversible uptake process, which in turn ensures the applicability of this technique to biomonitor long-term exposure. As a conclusion, the assessment of gaseous mercury pollution based on biomonitoring using S. alba has proven to be a useful, reliable and cost-effective methodology. Copyright © 2017 Elsevier Ltd. All rights reserved.

Towards improved biomonitoring tools for an intensified sustainable multi-use environment.

PubMed

van der Meer, Jan Roelof

2016-09-01

The increasing use of our environment for multiple contrasting activities (e.g. fisheries, tourism) will have to be accompanied by improved monitoring of environmental quality, to avoid transboundary conflicts and ensure long-term sustainable intensified usage. Biomonitoring approaches are appropriate for this, since they can integrate biological effects of environmental exposure rather than measure individual compound concentrations. Recent advances in biomonitoring concepts and tools focus on single-cell assays and purified biological components that can be miniaturized and integrated in automated systems. Despite these advances, we are still very far from being able to deploy bioassays routinely in environmental monitoring, mostly because of lack of experience in interpreting responses and insufficient robustness of the biosensors for their environmental application. Further future challenges include broadening the spectrum of detectable compounds by biosensors, accelerate response times and combining sample pretreatment strategies with bioassays. © 2016 The Author. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

The Ontario Benthos Biomonitoring Network

Treesearch

Chris Jones; Brian Craig; Nicole Dmytrow

2006-01-01

Canada’s Ontario Ministry of the Environment and Environment Canada (Ecological Monitoring and Assessment Network) are developing an aquatic macroinvertebrate biomonitoring network for Ontario’s lakes, streams, and wetlands. We are building the program, called the Ontario Benthos Biomonitoring Network (OBBN), on the principles of partnership, free data sharing, and...

Bio-Monitoring of Ozone by Young Students

ERIC Educational Resources Information Center

Lorenzini, Giacomo; Nali, Cristina

2004-01-01

An educational pilot project on the bio-monitoring of air quality was carried out in the Umbria Region of Central Italy. It involved about 1000 young students (ages 4 to 16) from 42 schools of 16 municipalities in active biomonitoring of tropospheric ozone with bio-indicator sensitive tobacco seedlings. Some 6500 raw biological readings were used…

The Use of Biomonitoring Data in Exposure and Human Health Risk Assessment: BENZENE CASE STUDY.

EPA Science Inventory

HESI Biomonitoring Technical Committee A framework of "Common Criteria" (i.e., a series of questions) has been developed to inform the use and evaluation of biomonitoring data in the context of human exposure and risk assessment (Albertini et al., 2006). The data-rich chemical b...

Biomonitoring of air pollution as exemplified by recent IAEA programs.

PubMed

Smodis, B; Parr, R M

1999-01-01

Biomonitoring is an appropriate tool for assessing the levels of atmospheric pollution, having several advantages compared with the use of direct measurements of contaminants (e.g., in airborne particulate matter, atmospheric deposition, precipitation), related primarily to the permanent and common occurrence of the chosen organisms in the field, the ease of sampling, and trace element accumulation. Furthermore, biomonitors may provide a measure of integrated exposure over an extended period of time and are present in remote areas and no expensive technical equipment is involved in collecting them. They accumulate contaminants over the exposure time and concentrate them, thus facilitating analytical measurements. Based on large-scale biomonitoring surveys, polluted areas can be identified, and by applying appropriate statistical tools, information can be obtained on the type of pollution sources and on the transboundary transport of atmospheric pollutants. The International Atomic Energy Agency is including the research on biomonitors in its projects on health-related environmental studies. Biomonitoring activities from several coordinated research projects on air pollution are presented, and results from an international workshop are discussed. In addition, activities in supporting improvement quality in the participating laboratories are outlined.

Interpretation of biomonitoring data in clinical medicine and the exposure sciences

DOE Office of Scientific and Technical Information (OSTI.GOV)

Williams, Bryan L.; Barr, Dana B.; Wright, J. Michael

2008-11-15

Biomonitoring has become a fundamental tool in both exposure science and clinical medicine. Despite significant analytical advances, the clinical use of environmental biomarkers remains in its infancy. Clinical use of environmental biomarkers poses some complex scientific and ethical challenges. The purpose of this paper is compare how the clinical and exposure sciences differ with respect to their interpretation and use of biological data. Additionally, the clinical use of environmental biomonitoring data is discussed. A case study is used to illustrate the complexities of conducting biomonitoring research on highly vulnerable populations in a clinical setting.

Macroinvertebrate biomonitoring in intermittent coastal plain streams impacted by animal agriculture.

PubMed

Davis, Stephanie; Golladay, Stephen W; Vellidis, George; Pringle, Catherine M

2003-01-01

Little attention has been given to the ecology of intermittent coastal plain streams in the southeastern United States, and it is not known whether available macroinvertebrate biomonitoring methods reliably detect degradation in these streams. This study compared differences in biomonitoring metrics between reference and agricultural streams, and between the flow period (January-April) and the intermittent flow period (May-December). Percentages of crustaceans, isopods, and Ephemeroptera-Plecoptera-Trichoptera (EPT) were significantly higher at the reference site than the two most impacted sites during the flow period, probably resulting from the abundance of leaf litter and lower temperatures. During this same period, the agriculturally impacted sites had a significantly higher percentage of dipterans--a group that thrives in the silty, nutrient-rich waters. Four metrics (percent Crustacea, Isopoda, Diptera, and EPT) had no overlap between values for the most impacted and the least impacted sites during the flow period, but no metrics were able to detect more discrete differences among sites. Sites were physically and biologically similar during the intermittent period when natural stresses (i.e., stagnant water, high temperatures, low dissolved oxygen) were high, with many metrics, such as percentages of dominant family, burrowers, chironomids, and dipterans becoming similar at all sites. Our findings indicate that development of a better understanding of invertebrate fauna in reference conditions and of the natural variation in intermittent streams is necessary to develop effective biomonitoring programs for these systems.

Citizens' Guide to Biomonitoring in the Great Lakes and St. Lawrence River.

ERIC Educational Resources Information Center

Great Lakes United, Buffalo, NY.

The purpose of this report is to present the issues surrounding biomonitoring of wastewaters discharged into the Great Lakes Basin. Biomonitoring is the process of using organisms to monitor the toxicity of a substance. The report reflects an interest in seeing zero discharge of toxic pollutants in the Great Lakes region. The report is organized…

Biomonitoring Data for 2,4-Dichlorophenoxyacetic Acid in the United States and Canada: Interpretation in a Public Health Risk Assessment Context Using Biomonitoring Equivalents

EPA Science Inventory

Several extensive studies of exposure to 2,4-dichlorophenoxyacetic acid (2,4-D) using urinary concentrations in samples from the general population, farm applicators, and farm family members are now available. Reference doses (RfDs) exist for 2,4-D, and Biomonitoring Equivalents ...

Biomonitoring: Measuring Toxins in Our Bodies as a Tool in Protecting Children's Health

ERIC Educational Resources Information Center

Patton, Sharyle

2005-01-01

Biomonitoring is a public health tool that has been used by scientists and researchers for decades to test blood, bone, urine, hair, human milk, adipose tissue, and other body substances for the presence of toxic chemicals, in order to assess what is called the "chemical body burden." Biomonitoring helps to: (1) identify which chemicals…

Biomonitoring Data for 2,4-Dichlorophenoxyacetic Acid in the United States and Canada: Interpretation in a Public Health Risk Assessment Context Using Biomonitoring Equivalents

PubMed Central

Aylward, Lesa L.; Morgan, Marsha K.; Arbuckle, Tye E.; Barr, Dana B.; Burns, Carol J.; Alexander, Bruce H.; Hays, Sean M.

2010-01-01

Background Several extensive studies of exposure to 2,4-dichlorophenoxyacetic acid (2,4-D) using urinary concentrations in samples from the general population, farm applicators, and farm family members are now available. Reference doses (RfDs) exist for 2,4-D, and Biomonitoring Equivalents (BEs; concentrations in urine or plasma that are consistent with those RfDs) for 2,4-D have recently been derived and published. Objective We reviewed the available biomonitoring data for 2,4-D from the United States and Canada and compared them with BE values to draw conclusions regarding the margin of safety for 2,4-D exposures within each population group. Data sources Data on urinary 2,4-D excretion in general and target populations from recent published studies are tabulated and the derivation of BE values for 2,4-D summarized. Data synthesis The biomonitoring data indicate margins of safety (ratio of BE value to biomarker concentration) of approximately 200 at the central tendency and 50 at the extremes in the general population. Median exposures for applicators and their family members during periods of use appear to be well within acute exposure guidance values. Conclusions Biomonitoring data from these studies indicate that current exposures to 2,4-D are below applicable exposure guidance values. This review demonstrates the value of biomonitoring data in assessing population exposures in the context of existing risk assessments using the BE approach. Risk managers can use this approach to integrate the available biomonitoring data into an overall assessment of current risk management practices for 2,4-D. PMID:20123603

A proposed framework for the interpretation of biomonitoring data

PubMed Central

Boogaard, Peter J; Money, Chris D

2008-01-01

Biomonitoring, the determination of chemical substances in human body fluids or tissues, is more and more frequently applied. At the same time detection limits are decreasing steadily. As a consequence, many data with potential relevance for public health are generated although they need not necessarily allow interpretation in term of health relevance. The European Centre of Ecotoxicology and Toxicology of Chemicals (ECETOC) formed a dedicated task force to build a framework for the interpretation of biomonitoring data. The framework that was developed evaluates biomonitoring data based on their analytical integrity, their ability to describe dose (toxicokinetics), their ability to relate to effects, and an overall evaluation and weight of evidence analysis. This framework was subsequently evaluated with a number of case studies and was shown to provide a rational basis to advance discussions on human biomonitoring allowing better use and application of this type of data in human health risk assessment. PMID:18541066

Rapid toxicity detection in water quality control utilizing automated multispecies biomonitoring for permanent space stations

NASA Technical Reports Server (NTRS)

Morgan, E. L.; Young, R. C.; Smith, M. D.; Eagleson, K. W.

1986-01-01

The objective of this study was to evaluate proposed design characteristics and applications of automated biomonitoring devices for real-time toxicity detection in water quality control on-board permanent space stations. Simulated tests in downlinking transmissions of automated biomonitoring data to Earth-receiving stations were simulated using satellite data transmissions from remote Earth-based stations.

Screening of seaweeds in the East China Sea as potential bio-monitors of heavy metals.

PubMed

Pan, Yaoru; Wernberg, Thomas; de Bettignies, Thibaut; Holmer, Marianne; Li, Ke; Wu, Jiaping; Lin, Fang; Yu, Yan; Xu, Jiang; Zhou, Chaosheng; Huang, Zhixing; Xiao, Xi

2018-06-01

Seaweeds are good bio-monitors of heavy metal pollution and have been included in European coastal monitoring programs. However, data for seaweed species in China are scarce or missing. In this study, we explored the potential of seaweeds as bio-monitor by screening the natural occurring seaweeds in the "Kingdom of seaweed and shellfish" at Dongtou Islands, the East China Sea. Totally, 12 seaweed species were collected from six sites, with richness following the sequence of Rhodophyta > Phaeophyta > Chlorophyta. The concentration of heavy metals (Cu, Cr, Ni, Zn, Pb, Cd, As) in the seaweeds was determined, and the bioaccumulation coefficient was calculated. A combination of four seaweeds, Pachydictyon coriaceum, Gelidium divaricatum, Sargassum thunbergii, and Pterocladiella capillacea, were proposed as bio-monitors due to their high bioaccumulation capabilities of specific heavy metals in the East China Sea and hence hinted the importance of using seaweed community for monitoring of pollution rather than single species. Our results provide first-hand data for the selection of bio-monitor species for heavy metals in the East China Sea and contribute to selection of cosmopolitan bio-monitor communities over geographical large area, which will benefit the establishment of monitoring programs for coastal heavy metal contamination.

Source water monitoring and biomonitoring systems

EPA Science Inventory

Presentation will provide background information on continuous source water monitoring using online toxicity monitors and cover various tools available. Conceptual and practical aspects of source water quality monitoring will be discussed.

Biomonitoring of airborne particulate matter emitted from a cement plant and comparison with dispersion modelling results

NASA Astrophysics Data System (ADS)

Abril, Gabriela A.; Wannaz, Eduardo D.; Mateos, Ana C.; Pignata, María L.

2014-01-01

The influence of a cement plant that incinerates industrial waste on the air quality of a region in the province of Córdoba, Argentina, was assessed by means of biomonitoring studies (effects of immission) and atmospheric dispersion (effects of emission) of PM10 with the application of the ISC3 model (Industrial Source Complex) developed by the USEPA (Environmental Protection Agency). For the biomonitoring studies, samples from the epiphyte plant Tillandsia capillaris Ruíz & Pav. f. capillaris were transplanted to the vicinities of the cement plant in order to determine the physiological damage and heavy metal accumulation (Ca, Mn, Fe, Co, Ni, Cu, Zn, Cd and Pb). For the application of the ISC3 model, point and area sources from the cement plant were considered to obtain average PM10 concentration results from the biomonitoring exposure period. This model permitted it to be determined that the emissions from the cement plant (point and area sources) were confined to the vicinities, without significant dispersion in the study area. This was also observed in the biomonitoring study, which identified Ca, Cd and Pb, pH and electric conductivity (EC) as biomarkers of this cement plant. Vehicular traffic emissions and soil re-suspension could be observed in the biomonitors, giving a more complete scenario. In this study, biomonitoring studies along with the application of atmospheric dispersion models, allowed the atmospheric pollution to be assessed in more detail.

Biomarker responses and contamination levels in crabs (Carcinus aestuarii) from the Lagoon of Venice: An integrated approach in biomonitoring estuarine environments.

PubMed

Ricciardi, Francesco; Matozzo, Valerio; Binelli, Andrea; Marin, Maria Gabriella

2010-03-01

An integrated biological-chemical approach is necessary to evaluate correctly the environmental status of bodies of water, as suggested by the EU Water Framework Directive. The shore crab Carcinus aestuarii, sampled in the Lagoon of Venice (NE Italy), was used as a biomonitor species, and the chemical concentrations of 42 organic pollutants (HCHs, PAHs, PCBs, DDTs, PBDEs), biological responses related to neurotoxicity (AChE inhibition), detoxification mechanisms (CYP450 induction) and endocrine alterations (vitellogenin-like protein induction) were measured at the same time. The responsiveness of biomarkers as predictors (or descriptors) of chemical contamination was evaluated by multivariate regression analysis, revealing good predictor potential for the selected biomarkers. Biomonitoring in the Lagoon of Venice revealed a predominance of DDT and PCB compounds, especially near industrial sites or large cities. Endocrine alterations, not always correlated with the presence of measured compounds, were also detected in many areas, suggesting exposure to compounds able to interfere with the crab endocrine system. Copyright 2009 Elsevier Ltd. All rights reserved.

Foreword: Biomonitoring Equivalents special issue.

PubMed

Meek, M E; Sonawane, B; Becker, R A

2008-08-01

The challenge of interpreting results of biomonitoring for environmental chemicals in humans is highlighted in this Foreword to the Biomonitoring Equivalents (BEs) special issue of Regulatory Toxicology and Pharmacology. There is a pressing need to develop risk-based tools in order to empower scientists and health professionals to interpret and communicate the significance of human biomonitoring data. The BE approach, which integrates dosimetry and risk assessment methods, represents an important advancement on the path toward achieving this objective. The articles in this issue, developed as a result of an expert panel meeting, present guidelines for derivation of BEs, guidelines for communication using BEs and several case studies illustrating application of the BE approach for specific substances.

Assessment of air pollution impacts on vegetation in South Africa

DOE Office of Scientific and Technical Information (OSTI.GOV)

Botha, A.T.

1989-01-01

Field surveys and biomonitoring network experiments were conducted in selected areas in South Africa to assess possible air pollution damage to vegetation. During field surveys, atmospheric fluoride was identified as an important pollutant that damaged vegetation in residential areas north of Cape Town. Gaseous air pollutants, including acid deposition and acidic mist, probably play a major role in the development of characteristic air pollution injury symptoms observed on pine trees in the Eastern Transvaal area. The impact of urban air pollution in the Cape Town area was evaluated by exposing bio-indicator plants in a network of eight biomonitoring network stationsmore » from June 1985 to May 1988. Sensitive Freesia and Gladiolus cultivars were used to biomonitor atmospheric fluoride, while a green bean cultivar was used as a biomonitor of atmospheric sulfur dioxide and ozone. At one location, bio-indicator plants were simultaneously exposed in a biomonitoring network station, open-top chambers, as well as in open plots. The responses of plants grown under these different conditions were compared.« less

Evaluation of Biomonitoring Data from the CDC National ...

EPA Pesticide Factsheets

BACKGROUND: Biomonitoring data reported in the National Report on Human Exposure to Environmental Chemicals (NER) provide information on the presence and concentrations of more than 400 chemicals in human blood and urine. Biomonitoring Equivalents (BEs) and other risk assessment-based values now allow interpretation of these biomonitoring data in a public health risk context. OBJECTIVES: Compare the measured biomarker concentrations in the NER with BEs and similar risk assessment values to provide an across-chemical risk assessment perspective on the measured levels for approximately 130 analytes in the NER. METHODS: Available risk assessment-based biomarker screening values, including BEs and Human Biomonitoring-I (HBM-I) values from the German Human Biomonitoring Commission, were identified. Geometric mean and 95th percentile population biomarker concentrations from the NER were compared to the available screening values to generate chemical-specific hazard quotients (HQ) or cancer risk estimates. CONCLUSIONS: Several analytes in the NER approach or exceed HQ values of 1 or cancer risks greater than 1x 10-4 at the geometric mean or 95th percentile, suggesting exposure levels exceed what is considered safe in a large fraction of the population. Analytes of concern include acrylamide, dioxin-like chemicals, benzene, xylene, several metals, di-2(ethylhexyl)phthalate, and some legacy organochlorine pesticides. This analysis provides for the first time a mean

US Fish and Wildlife Service lands biomonitoring operations manual

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rope, R.C.; Breckenridge, R.P.

1993-08-01

This is Volume 1 of an operations manual designed to facilitate the development of biomonitoring strategies for U.S. Fish and Wildlife Service Lands. It is one component of the U.S. Fish and Wildlife Service Lands Biomonitoring Operations Manual. The Volume contains the Introduction to the Manual, background information on monitoring, and procedures for developing a biomonitoring strategy for Service lands. The purpose of the Biomonitoring Operations Manual is to provide an approach to develop and implement biomonitoring activities to assess the status and trends of U.S. Fish and Wildlife Service trust resources. It also provides field sampline methods and documentationmore » protocols for contaminant monitoring activities. The strategy described in the Manual has been designed as a stand alone process to characterize the presence of contaminants on lands managed by the Service. This process can be sued to develop a monitoring program for any tract of real estate with potential threats from on- or off-site contaminants. Because the process was designed to address concerns for Service lands that span the United States from Alaska to the Tropical Islands, it has a generic format that can be used in al types of ecosystems, however, significant site specific informtion is required to complete the Workbook and make the process work successfully.« less

Interpreting biomonitoring data for 2,4-dichlorophenoxyacetic acid: Update to Biomonitoring Equivalents and population biomonitoring data.

PubMed

Aylward, L L; Hays, S M

2015-12-01

Urinary biomonitoring data for 2,4-dichlorophenoxyacetic acid (2,4-D) reflect aggregate population exposures to trace 2,4-D residues in diet and the environment. These data can be interpreted in the context of current risk assessments by comparison to a Biomonitoring Equivalent (BE), which is an estimate of the average biomarker concentration consistent with an exposure guidance value such as the US EPA Reference Dose (RfD). BE values are updated here from previous published BE values to reflect a change in the US EPA RfD. The US EPA RfD has been updated to reflect a revised point of departure (POD) based on new information from additional toxicological studies and updated assessment of applicable uncertainty factors. In addition, new biomonitoring data from both the US National Health and Nutrition Examination Survey (NHANES) and the Canadian Health Measures Survey (CHMS) have been published. The updated US EPA chronic RfD of 0.21 mg/kg-d results in updated BE values of 10,500 and 7000 μg/L for adults and children, respectively. Comparison of the current population-representative data to these BE values shows that upper bound population biomarker concentrations are more than 5000-fold below BE values corresponding to the updated US EPA RfD. This biomonitoring-based risk assessment supports the conclusion that current use patterns in the US and Canada result in incidental exposures in the general population that can be considered negligible in the context of the current 2,4-D risk assessment. Copyright © 2015 Elsevier Inc. All rights reserved.

Environmental Sentinel Biomonitor (ESB) System Technology Assessment

DTIC Science & Technology

2007-02-01

Andersson M, Barile FA, et al. 1996. MEIC evaluation of acute systemic toxicity Part I. Methodology of 68 in vitro toxicity assays used to test the first...30 reference chemicals. ATLA 24:251-272. Clemedson C, Andersson M, Aoki Y, Barile FA, et al. 1998. MEIC evaluation of acute systemic toxicity Part IV

Exposure Assessment and Biomonitoring of Workers in Magnetic Resonance Environment: An Exploratory Study

PubMed Central

Sannino, Anna; Romeo, Stefania; Scarfì, Maria Rosaria; Massa, Rita; d’Angelo, Raffaele; Petrillo, Antonella; Cerciello, Vincenzo; Fusco, Roberta; Zeni, Olga

2017-01-01

Magnetic resonance imaging (MRI) has evolved rapidly over the past few decades as one of the most flexible tools in medical research and diagnostic imaging. MRI facilities are important sources of multiple exposure to electromagnetic fields for both patients and health-care staff, due to the presence of electromagnetic fields of multiple frequency ranges, different temporal variations, and field strengths. Due to the increasing use and technological advancements of MRI systems, clearer insights into exposure assessment and a better understanding of possible harmful effects due to long-term exposures are highly needed. In the present exploratory study, exposure assessment and biomonitoring of MRI workers at the Radio-diagnostics Unit of the National Cancer Institute of Naples “Pascale Foundation” (Naples, Italy) have been carried out. In particular, exposure to the MRI static magnetic field (SMF) has been evaluated by means of personal monitoring, while an application tool has been developed to provide an estimate of motion-induced, time-varying electric fields. Measurement results have highlighted a high day-to-day and worker-to-worker variability of the exposure to the SMF, which strongly depends on the characteristics of the environment and on personal behaviors, and the developed application tool can be adopted as an easy-to-use tool for rapid and qualitative evaluation of motion-induced, time-varying electric field exposure. Regarding biomonitoring, the 24 workers of the Radio-diagnostics Unit were enrolled to evaluate both spontaneous and mitomycin C-induced chromosomal fragility in human peripheral blood lymphocytes, by means of the cytokinesis-block micronucleus assay. The study subjects were 12 MRI workers, representative of different professional categories, as the exposed group, and 12 workers with no MRI exposure history, as the reference group. The results show a high worker-to-worker variability for both field exposure assessment and biomonitoring, as well as several critical issues and practicalities to be faced with in this type of investigations. The procedures for risk assessment and biomonitoring proposed here can be used to inform future research in this field, which will require a refinement of exposure assessment methods and an enlargement of the number of subjects enrolled in the biomonitoring study to gain robust statistics and reliable results. PMID:29326919

A WATERSHED APPROACH TO DRINKING WATER QUALITY

EPA Science Inventory

The purpose of this presentation is to describe emerging technologies and strategies managing watersheds with the goal of protecting drinking water sources. Included are discussions on decentralized wastewater treatment, whole organism biomonitor detection systems, treatment of...

Biomonitoring of Non-Dioxin-Like Polychlorinated Biphenyls in Transgenic Arabidopsis Using the Mammalian Pregnane X Receptor System: A Role of Pectin in Pollutant Uptake

PubMed Central

Bao, Lieming; Gao, Chen; Li, Miaomiao; Chen, Yong; Lin, Weiqiang; Yang, Yanjun; Han, Ning; Bian, Hongwu; Zhu, Muyuan; Wang, Junhui

2013-01-01

Polychlorinated biphenyls (PCBs) are persistent organic pollutants damaging to human health and the environment. Techniques to indicate PCB contamination in planta are of great interest to phytoremediation. Monitoring of dioxin-like PCBs in transgenic plants carrying the mammalian aryl hydrocarbon receptor (AHR) has been reported previously. Herein, we report the biomonitoring of non-dioxin-like PCBs (NDL-PCBs) using the mammalian pregnane X receptor (PXR). In the transgenic Arabidopsis designated NDL-PCB Reporter, the EGFP-GUS reporter gene was driven by a promoter containing 18 repeats of the xenobiotic response elements, while PXR and its binding partner retinoid X receptor (RXR) were coexpressed. Results showed that, in live cells, the expression of reporter gene was insensitive to endogenous lignans, carotenoids and flavonoids, but responded to all tested NDL-PCBs in a dose- and time- dependent manner. Two types of putative PCB metabolites, hydroxy- PCBs and methoxy- PCBs, displayed different activation properties. The vascular tissues seemed unable to transport NDL-PCBs, whereas mutation in QUASIMODO1 encoding a 1,4-galacturonosyltransferase led to reduced PCB accumulation in Arabidopsis, revealing a role for pectin in the control of PCB translocation. Taken together, the reporter system may serve as a useful tool to biomonitor the uptake and metabolism of NDL-PCBs in plants. PMID:24236133

[Air pollution biomonitoring with plants and fungi: concepts and uses].

PubMed

Cuny, D

2012-07-01

Air pollution remains a major environmental concern of the French. Since about 30 years, due to evolution and diversification of sources, pollution became more and more complex, constituting a true "cocktail". Today, it is very important to know environmental and health effects of this cocktail. In this context air biomonitoring using plants and fungi can bring a lot of information. Biomonitoring includes four concepts: the use of biomarkers, bioindication biointegration and bioaccumulation. These four concepts are articulated according to the levels of biological organization, what links up biosurveillance on fundamental plan with ecotoxicology. It is a complementary approach of the physicochemical techniques of air pollution measurements. The main objectives of biomonitoring studies are the monitoring of the space and temporal distribution of pollutants effect; the monitoring of local sources; participation in the health risks assessment; the information of people and the help to decision in public policies. Biomonitoring of air quality is a method, which made its proof in numerous domains of application and brings fundamental information on the impacts of the quality of air. Recent evolution of low concerning biggest industries allows us to envisage the increase of air quality biomonitoring with plants and fungi applications in the field of the valuation of environmental and health risks. The recent normalization (French and European) of different methods will also allow the development of uses. Copyright © 2012. Published by Elsevier Masson SAS.

Biomonitoring of 2,4,6-trinitrotoluene and degradation products in the marine environment with transplanted blue mussels (M. edulis).

PubMed

Strehse, Jennifer S; Appel, Daniel; Geist, Catharina; Martin, Hans-Jörg; Maser, Edmund

2017-09-01

Since World War I considerable amounts of warfare material have been dumped at sea worldwide, but little is known about the fate of the explosive components in the marine environment. Sea dumped munitions are able to contaminate the surroundings because of the release of explosive chemicals due to corrosion and breaching or by detonation after blast-operations. This implies the risk of accumulation of toxic compounds in human and wildlife food chains. With the help of divers, we performed an active biomonitoring study with transplanted blue mussels (M. edulis) in a burdened area (Kolberger Heide, Germany) with explosive compounds near blast craters over an exposure time of 93days. With this biomonitoring system, we could show that blue mussels accumulate 2,4,6-trinitrotoluene (TNT) and its metabolites 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT) in their tissues. In all mussels deployed at the ground, we found a body burden with 2-ADNT of 103.75±12.77ng/g wet weight and with 4-ADNT of 131.31±9.53ng/g wet weight. TNT itself has been found in six mussels with an average concentration of 31.04±3.26ng/g mussel wet weight. In the mussels positioned at one meter above the ground no TNT nor 2-ADNT could be detected, but 4-ADNT was found in all samples with an average concentration of 8.71±2.88ng/g mussel wet weight. To the best of our knowledge, this is the first study using blue mussels M. edulis as an active biomonitoring system for TNT and its metabolites 2-ADNT and 4-ADNT in a free field experiment in a burdened area. Moreover, with this system, we unequivocally proved that these toxic explosives accumulate in the marine biota resp. in the marine food chain, thereby posing a possible risk to the marine ecosphere and human health. Copyright © 2017 Elsevier B.V. All rights reserved.

US Fish and Wildlife Service biomonitoring operations manual, Appendices A--K

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gianotto, D.F.; Rope, R.C.; Mondecar, M.

1993-04-01

Volume 2 contains Appendices and Summary Sheets for the following areas: A-Legislative Background and Key to Relevant Legislation, B- Biomonitoring Operations Workbook, C-Air Monitoring, D-Introduction to the Flora and Fauna for Biomonitoring, E-Decontamination Guidance Reference Field Methods, F-Documentation Guidance, Sample Handling, and Quality Assurance/Quality Control Standard Operating Procedures, G-Field Instrument Measurements Reference Field Methods, H-Ground Water Sampling Reference Field Methods, I-Sediment Sampling Reference Field Methods, J-Soil Sampling Reference Field Methods, K-Surface Water Reference Field Methods. Appendix B explains how to set up strategy to enter information on the ``disk workbook``. Appendix B is enhanced by DE97006389, an on-line workbook formore » users to be able to make revisions to their own biomonitoring data.« less

Monitoring population exposure to pesticides based on liquid chromatography-tandem mass spectrometry measurement of their urinary metabolites in urban wastewater: A novel biomonitoring approach.

PubMed

Rousis, Nikolaos I; Zuccato, Ettore; Castiglioni, Sara

2016-11-15

Biomonitoring studies have documented the high exposure of the population to pesticides which are widely used for crop protection, industrial and household purposes. This is the first study which has measured human urinary metabolites of pesticides in urban wastewater as biomarkers of population exposure. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to measure fifteen urinary metabolites selected from the major classes of pesticides. Raw wastewater samples were processed by solid phase extraction (SPE) or direct injection into the LC-MS/MS system. Recoveries ranged from 75 to 115% and the limits of quantification were 1-15ng/L for the SPE method and 40-800ng/L for direct injection. The method was employed for the analysis of 44 composite 24-h wastewater samples collected in seven Italian cities. Most of the target substances were detected at concentrations ranging from 1.1ng/L to 1.6μg/L. The highest concentrations were for some common metabolites of alkyl phosphates and pyrethroids and the specific metabolite of chlorpyrifos and chlorpyrifos-methyl (3,5,6-trichloro-2-pyridinol). The frequency of detection and abundance of most of the measured metabolites were in line with the profiles reported in urine biomonitoring studies. This method is therefore proposed as a novel "biomonitoring approach" for obtaining objective, direct information on the levels of exposure of a specific population to pesticides, and current research is addressed to validate the method identifying the most reliable biomarkers. Copyright © 2016 Elsevier B.V. All rights reserved.

Nitrogen multitemporal monitoring through mosses in urban areas affected by mud volcanoes around Mt. Etna, Italy.

PubMed

Bonanno, Giuseppe

2013-10-01

Nitrogen emissions were assessed by using mosses as bioindicators in a densely inhabited area affected by mud volcanoes. Such volcanoes, locally called Salinelle, are phenomena that occur around Mt. Etna (Sicily, Italy), and are interpreted as the surface outflow of a hydrothermal system located below Mt. Etna, which releases sedimentary fluids (hydrocarbons and Na-Cl brines) along with magmatic gases (mainly CO2 and He). To date, N emissions from such mud volcanoes have been only quantitatively assessed, and no biomonitoring campaigns are reported about the cumulative effects of these emissions. This study analyzed N concentrations in moss, water and soil samples, collected in a 4-year monitoring campaign. The bryophyte Bryum argenteum, a species widely adopted in surveys of atmospheric pollution, was used as a biological indicator. N concentrations in biomonitors showed relatively low values in the study sites. However, the results of this study suggest that N emissions from Salinelle may have an impact on surrounding ecosystems because N values in moss and water showed a significant correlation. N oxides, in particular, contribute to acidification of ecosystems, thus multitemporal biomonitoring is recommended, especially in those areas where N emitting sources are anthropogenic and natural.

Determination of endocrine-disrupting chemicals in human milk by dispersive liquid-liquid microextraction.

PubMed

Vela-Soria, Fernando; Jiménez-Díaz, Inmaculada; Díaz, Caridad; Pérez, José; Iribarne-Durán, Luz María; Serrano-López, Laura; Arrebola, Juan Pedro; Fernández, Mariana Fátima; Olea, Nicolás

2016-09-01

Human populations are widely exposed to numerous so-called endocrine-disrupting chemicals, exogenous compounds able to interfere with the endocrine system. This exposure has been associated with several health disorders. New analytical procedures are needed for biomonitoring these xenobiotics in human matrices. A quick and inexpensive methodological procedure, based on sample treatment by dispersive liquid-liquid microextraction, is proposed for the determination of bisphenols, parabens and benzophenones in samples. LOQs ranged from 0.4 to 0.7 ng ml(-1) and RSDs from 4.3 to 14.8%. This methodology was satisfactorily applied in the simultaneous determination of a wide range of endocrine-disrupting chemicals in human milk samples and is suitable for application in biomonitoring studies.

USING BIOMONITORING DATA TO INFORM EXPOSURE ASSESSMENT IN CHILDREN

EPA Science Inventory

Discussing the challenges associated with estimating and interpreting toxicant exposures and health risks from biomonitoring data. Extended abstract will also be translated in Spanish and published in Acta Toxicologica Argentina.

Expanding Genomic Biomonitoring to Regional & National Scale Projects

EPA Science Inventory

This presentation provides a summary of ORD/NERL/SED research efforts applying DNA metabarcoding to questions related to biomonitoring and invasive species detection, along with development of sensitive indicators of nutrient condition.

EVALUATION OF MULTIPLE AQUATIC BIOMONITORS FOR SOURCE WATER PROTECTION

EPA Science Inventory

A variety of probes for use in continuous monitoring of water quality exist. They range from single parameter chemical/physical probes to comprehensive screening systems based on whole organism responses. Originally developed for monitoring specific characteristics of water qua...

A Biomonitoring Framework to Support Exposure and Risk Assessments

EPA Science Inventory

Background - Biomonitoring is used in exposure and risk assessments to reduce uncertainties along the source-to-outcome continuum. Specifically, biomarkers can help identify exposure sources, routes, and distributions, and reflect kinetic and dynamic processes following exposure ...

Environmental health and justice and the right to research: institutional review board denials of community-based chemical biomonitoring of breast milk.

PubMed

Saxton, Dvera I; Brown, Phil; Seguinot-Medina, Samarys; Eckstein, Lorraine; Carpenter, David O; Miller, Pamela; Waghiyi, Vi

2015-11-25

Recently, conflicts and challenges have emerged regarding environmental justice and research ethics for some indigenous communities. Alaska Community Action on Toxics (ACAT) responded to community requests for breast milk biomonitoring and conceived the Breast Milk Pilot Study (BMPS). Despite having community support and federal and private funding, the BMPS remains incomplete due to repeated disapprovals by the Alaska Area IRB (Institutional Review Board). In this commentary, we explore the consequences of years of IRB denials, in terms of health inequalities, environmental justice, and research ethics. We highlight the greater significance of this story with respect to research in Alaska Native communities, biomonitoring, and global toxics regulation. We offer suggestions to community-based researchers conducting biomonitoring projects on how to engage with IRBs in order to cultivate reflective, context-based research ethics that better consider the needs and concerns of communities.

The use of vegetables in the biomonitoring of cadmium and lead pollution in the environment.

PubMed

Szczygłowska, Marzena; Bodnar, Małgorzata; Namieśnik, Jacek; Konieczka, Piotr

2014-01-01

Lead and cadmium emitted from various anthropogenic sources have the ability to accumulate in tissues of living organisms. The phenomenon of accumulation of metals in the body is harmful and undesirable. The ability of plants to accumulate heavy metals from the individual elements of the environment has been used in biomonitoring of pollution. Leaves and roots of vegetables have particular predisposition for accumulating toxic metals such as lead and cadmium and therefore can be used for biomonitoring of the environment, mainly as a tool for assessing the extent of soil contamination. The article discusses information in the literature on entry paths of lead and cadmium into the body, toxic effects of lead and cadmium on the human organism, and the use of vegetables as a tool in the biomonitoring of heavy metals in different elements of the environment.

Tillandsia usneoides: A successful alternative for biomonitoring changes in air quality due to a new highway in São Paulo, Brazil.

PubMed

Cardoso-Gustavson, Poliana; Fernandes, Francine Faia; Alves, Edenise Segala; Victorio, Mariana Pereira; Moura, Barbara Baesso; Domingos, Marisa; Rodrigues, Caroline Albuquerque; Ribeiro, Andreza Portella; Nievola, Catarina Carvalho; Figueiredo, Ana Maria G

2017-05-01

Tillandsia usneoides is an aerial epiphytic bromeliad that absorbs water and nutrients directly from the atmosphere by scales covering its surface. We expanded the use of this species as a broader biomonitor based on chemical and structural markers to detect changes in air quality. The usefulness of such comprehensive approach was tested during the construction and opening of a highway (SP-21) in São Paulo State, Brazil. The biomonitoring study was performed from 2009 to 2012, thus comprising the period during construction and after the highway inauguration. Metal accumulation and structural alterations were assessed, in addition to microscopy analyses to understand the metal chelation in plant tissues and to assess the causes of alterations in the number and shape of scale cells. Altogether, our analyses support the use of this species as a wide biomonitor of air quality in urbanized areas.

Tillandsia usneoides: a successful alternative for biomonitoring changes in air quality due to a new highway in São Paulo, Brazil.

PubMed

Cardoso-Gustavson, Poliana; Fernandes, Francine Faia; Alves, Edenise Segala; Victorio, Mariana Pereira; Moura, Barbara Baesso; Domingos, Marisa; Rodrigues, Caroline Albuquerque; Ribeiro, Andreza Portella; Nievola, Catarina Carvalho; Figueiredo, Ana Maria G

2016-01-01

Tillandsia usneoides is an aerial epiphytic bromeliad that absorbs water and nutrients directly from the atmosphere by scales covering its surface. We expanded the use of this species as a broader biomonitor based on chemical and structural markers to detect changes in air quality. The usefulness of such comprehensive approach was tested during the construction and opening of a highway (SP-21) in São Paulo State, Brazil. The biomonitoring study was performed from 2009 to 2012, thus comprising the period during construction and after the highway inauguration. Metal accumulation and structural alterations were assessed, in addition to microscopy analyses to understand the metal chelation in plant tissues and to assess the causes of alterations in the number and shape of scale cells. Altogether, our analyses support the use of this species as a wide biomonitor of air quality in urbanized areas.

Evaluating a 5-year metal contamination remediation and the biomonitoring potential of a freshwater gastropod along the Xiangjiang River, China.

PubMed

Li, Deliang; Pi, Jie; Zhang, Ting; Tan, Xiang; Fraser, Dylan J

2018-05-16

Effective remediation of heavy metal pollution in aquatic systems is desired in many regions, but it requires integrative assessments of sediments, water, and biota that can serve as robust biomonitors. We assessed the effects of a 5-year metal contamination remediation along the Xiangjiang River, China, by comparing concentrations of trace metals in water and surface sediments between 2010-2011 and 2016. We also explored the trace metal biomonitoring potential of a freshwater gastropod (Bellamya aeruginosa). Metal concentrations in water (means and ranges) dropped over time to within permissible limits of drinking water guidelines set by China, USEPA, and WHO in 2016. Although sediment means and ranges of Cd, Pb, Zn, and Mn also diminished with remediation, those for Cr and Cu slightly increased, and all six metals retained concentrations higher than standards set by China. All metals in sediments could also be associated with anthropogenic inputs using a hierarchical clustering analysis, and they generate high potential ecological risks based on several indices, especially for Cd and As. The bio-sediment accumulation factors of all measured trace metals in gastropod soft tissues and shells were lower than 1.0, except for Ca. Trace metal contents in gastropods were positively correlated with those in water and surface sediments for As (soft tissues) and Cr (shells). Collectively, our results do not yet highlight strong beneficial effects of 5-year remediation and clearly illustrate the heavy metal pollution remaining in Xiangjiang River sediment. Additional physical, chemical, and biological measurements should be implemented to improve sediment quality. We further conclude that gastropod soft tissues and shells can be suitable biomonitors of spatial differences in some heavy metals found within river sediments (e.g., As, Cr).

Urinary, Circulating, and Tissue Biomonitoring Studies Indicate Widespread Exposure to Bisphenol A

PubMed Central

Vandenberg, Laura N.; Chahoud, Ibrahim; Heindel, Jerrold J.; Padmanabhan, Vasantha; Paumgartten, Francisco J.R.; Schoenfelder, Gilbert

2010-01-01

Background Bisphenol A (BPA) is one of the highest-volume chemicals produced worldwide, and human exposure to BPA is thought to be ubiquitous. Thus, there are concerns that the amount of BPA to which humans are exposed may cause adverse health effects. Importantly, results from a large number of biomonitoring studies are at odds with the results from two toxicokinetic studies. Objective We examined several possibilities for why biomonitoring and toxicokinetic studies could come to seemingly conflicting conclusions. Data sources We examined > 80 published human biomonitoring studies that measured BPA concentrations in human tissues, urine, blood, and other fluids, along with two toxicokinetic studies of human BPA metabolism. Data extraction and synthesis The > 80 biomonitoring studies examined included measurements in thousands of individuals from several different countries, and these studies overwhelmingly detected BPA in individual adults, adolescents, and children. Unconjugated BPA was routinely detected in blood (in the nanograms per milliliter range), and conjugated BPA was routinely detected in the vast majority of urine samples (also in the nanograms per milliliter range). In stark contrast, toxicokinetic studies proposed that humans are not internally exposed to BPA. Some regulatory agencies have relied solely on these toxicokinetic models in their risk assessments. Conclusions Available data from biomonitoring studies clearly indicate that the general population is exposed to BPA and is at risk from internal exposure to unconjugated BPA. The two toxicokinetic studies that suggested human BPA exposure is negligible have significant deficiencies, are directly contradicted by hypothesis-driven studies, and are therefore not reliable for risk assessment purposes. PMID:20338858

Suitability of Tillandsia usneoides and Aechmea fasciata for biomonitoring toxic elements under tropical seasonal climate.

PubMed

Giampaoli, Patricia; Wannaz, Eduardo D; Tavares, Armando R; Domingos, Marisa

2016-04-01

Aechmea fasciata was evaluated for the first time as a biomonitor of toxic elements, in comparison to the biomonitoring capacity of Tillandsia usneoides, a well-established biomonitor bromeliad species. Plants of both species were exposed to air pollutants from industrial, urban, and agricultural sources, under the tropical seasonal climate, from June/2011 to April/2013, in five sites of São Paulo State, Brazil, for 8 consecutive exposure periods of 12 weeks each. The levels of essential and non-essential elements, including trace metals, were quantified at the end of each exposure. T. usneoides and A. fasciata indicated N, Fe, Zn, Co, Cr, and V as air contaminants in the studied sites, during wet and dry seasons and both species were recommended for qualitative biomonitoring. Concentration levels of N, Ca, S, Fe, Zn, Cu, B, Co, and Ni were significantly higher in T. usneoides than in A. fasciata. However, A. fasciata showed a higher effective retention capacity of Ni, Pb, V, Cu, Fe, Cr, and Co during field exposure, as indicated by the estimate of enrichment factor relative to basal concentrations. This species is more suitable for detecting the atmospheric pollution level of those metals than the T. usneoides. Both species indicated adequately the seasonal differences in the pollution levels of several elements, but T. usneoides presented higher ability for biomonitoring the spatial variations and for indicating more properly the sources of each element in the studied region than the A. fasciata. Copyright © 2016 Elsevier Ltd. All rights reserved.

Systems Biology and Biomarkers of Early Effects for Occupational Exposure Limit Setting

EPA Science Inventory

AbstractIn a recent National Research Council document, new strategies for risk assessment were described to enable more accurate and quicker assessments (()(1)()). This report suggested that evaluating individual responses through increased use of biomonitoring could improve dos...

Biomonitoring - An Exposure Science Tool for Exposure and Risk Assessment

EPA Science Inventory

Biomonitoring studies of environmental stressors are useful for confirming exposures, estimating dose levels, and evaluating human health risks. However, the complexities of exposure-biomarker and biomarker-response relationships have limited the use of biomarkers in exposure sc...

BIOMONITORING OF SOURCE WATER QUALITY

EPA Science Inventory

Living organisms are commonly used to determine the toxicity of environmental samples but are usually limited to survival, growth, or reproduction. With advances in electronic and computer technology, biomonitors are being developed that can assess the toxicity of water by monit...

An Environmental Sentinel Biomonitor System for Drinking Water Protection

DTIC Science & Technology

2008-12-01

threat chemicals. Potential interferences include chemicals commonly used for drinking water disinfection (chlorine and chloramine ), byproducts of...range. Of the potential interferences tested, the ECIS test was affected only by the disinfectants chlorine and chloramine ; chlorine is typically...Industrial and Potential Interfering Chemicals Used to Evaluate ESB System Toxicity Sensors TICs Potential Interferences Acrylonitrile Chloramine

Environmental sentinel biomonitors: integrated response systems for monitoring toxic chemicals

NASA Astrophysics Data System (ADS)

van der Schalie, William H.; Reuter, Roy; Shedd, Tommy R.; Knechtges, Paul L.

2002-02-01

Operational environments for military forces are becoming potentially more dangerous due to the increased number, use, and misuse of toxic chemicals across the entire range of military missions. Defense personnel may be exposed to harmful chemicals as a result of industrial accidents or intentional or unintentional action of enemy, friendly forces, or indigenous populations. While there has been a significant military effort to enable forces to operate safely and survive and sustain operations in nuclear, biological, chemical warfare agent environments, until recently there has not been a concomitant effort associated with potential adverse health effects from exposures of deployed personnel to toxic industrial chemicals. To provide continuous real-time toxicity assessments across a broad spectrum of individual chemicals or chemical mixtures, an Environmental Sentinel Biomonitor (ESB) system concept is proposed. An ESB system will integrate data from one or more platforms of biologically-based systems and chemical detectors placed in the environment to sense developing toxic conditions and transmit time-relevant data for use in risk assessment, mitigation, and/or management. Issues, challenges, and next steps for the ESB system concept are described, based in part on discussions at a September 2001 workshop sponsored by the U.S. Army Center for Environmental Health Research.

Transgenic Arabidopsis Gene Expression System

NASA Technical Reports Server (NTRS)

Ferl, Robert; Paul, Anna-Lisa

2009-01-01

The Transgenic Arabidopsis Gene Expression System (TAGES) investigation is one in a pair of investigations that use the Advanced Biological Research System (ABRS) facility. TAGES uses Arabidopsis thaliana, thale cress, with sensor promoter-reporter gene constructs that render the plants as biomonitors (an organism used to determine the quality of the surrounding environment) of their environment using real-time nondestructive Green Fluorescent Protein (GFP) imagery and traditional postflight analyses.

HUMAN BIOMONITORING TO LINK ENVIRONMENTAL EXPOSURE TO BIOLOGICALLY RELEVANT DOSE

EPA Science Inventory

The abstract and presentation on Human Biomonitoring to Link Environmental Exposure to Biologically Relevant Dose describes the use of biomarkers of exposure, biomarkers of current health state, and biomarker measurements. The abstract and presentation focuses on how biomarkers ...

Reconstructing Human Exposures Using Biomarkers and other "Clues"

EPA Science Inventory

Biomonitoring is the process by which biomarkers are measured in human tissues and specimens to evaluate exposures. Given the growing number of population-based biomonitoring surveys, there is now an escalated interest in using biomarker data to reconstruct exposures for supporti...

PHARMACOKINETIC MODELS IN THE DESIGN OF BIOMONITORING PROGRAMS

EPA Science Inventory

Measurements of chemicals in tissues, blood, or urine can be related to health effects because they are an integrated measure of absorbed dose following exposure. However, the direct relationship between biomonitoring data and pathway-specific exposures is more tenuous. In chem...

Toxicological importance of human biomonitoring of metallic and metalloid elements in different biological samples.

PubMed

Gil, F; Hernández, A F

2015-06-01

Human biomonitoring has become an important tool for the assessment of internal doses of metallic and metalloid elements. These elements are of great significance because of their toxic properties and wide distribution in environmental compartments. Although blood and urine are the most used and accepted matrices for human biomonitoring, other non-conventional samples (saliva, placenta, meconium, hair, nails, teeth, breast milk) may have practical advantages and would provide additional information on health risk. Nevertheless, the analysis of these compounds in biological matrices other than blood and urine has not yet been accepted as a useful tool for biomonitoring. The validation of analytical procedures is absolutely necessary for a proper implementation of non-conventional samples in biomonitoring programs. However, the lack of reliable and useful analytical methodologies to assess exposure to metallic elements, and the potential interference of external contamination and variation in biological features of non-conventional samples are important limitations for setting health-based reference values. The influence of potential confounding factors on metallic concentration should always be considered. More research is needed to ascertain whether or not non-conventional matrices offer definitive advantages over the traditional samples and to broaden the available database for establishing worldwide accepted reference values in non-exposed populations. Copyright © 2015 Elsevier Ltd. All rights reserved.

Toxic ignorance and right-to-know in biomonitoring results communication: a survey of scientists and study participants.

PubMed

Morello-Frosch, Rachel; Brody, Julia Green; Brown, Phil; Altman, Rebecca Gasior; Rudel, Ruthann A; Pérez, Carla

2009-02-28

Exposure assessment has shifted from pollutant monitoring in air, soil, and water toward personal exposure measurements and biomonitoring. This trend along with the paucity of health effect data for many of the pollutants studied raise ethical and scientific challenges for reporting results to study participants. We interviewed 26 individuals involved in biomonitoring studies, including academic scientists, scientists from environmental advocacy organizations, IRB officials, and study participants; observed meetings where stakeholders discussed these issues; and reviewed the relevant literature to assess emerging ethical, scientific, and policy debates about personal exposure assessment and biomonitoring, including public demand for information on the human health effects of chemical body burdens. We identify three frameworks for report-back in personal exposure studies: clinical ethics; community-based participatory research; and citizen science 'data judo.' The first approach emphasizes reporting results only when the health significance of exposures is known, while the latter two represent new communication strategies where study participants play a role in interpreting, disseminating, and leveraging results to promote community health. We identify five critical areas to consider in planning future biomonitoring studies. Public deliberation about communication in personal exposure assessment research suggests that new forms of community-based research ethics and participatory scientific practice are emerging.

Review of genotoxicity biomonitoring studies of glyphosate-based formulations

PubMed Central

Kier, Larry D.

2015-01-01

Abstract Human and environmental genotoxicity biomonitoring studies involving exposure to glyphosate-based formulations (GBFs) were reviewed to complement an earlier review of experimental genotoxicity studies of glyphosate and GBFs. The environmental and most of the human biomonitoring studies were not informative because there was either a very low frequency of GBF exposure or exposure to a large number of pesticides without analysis of specific pesticide effects. One pesticide sprayer biomonitoring study indicated there was not a statistically significant relationship between frequency of GBF exposure reported for the last spraying season and oxidative DNA damage. There were three studies of human populations in regions of GBF aerial spraying. One study found increases for the cytokinesis-block micronucleus endpoint but these increases did not show statistically significant associations with self-reported spray exposure and were not consistent with application rates. A second study found increases for the blood cell comet endpoint at high exposures causing toxicity. However, a follow-up to this study 2 years after spraying did not indicate chromosomal effects. The results of the biomonitoring studies do not contradict an earlier conclusion derived from experimental genotoxicity studies that typical GBFs do not appear to present significant genotoxic risk under normal conditions of human or environmental exposures. PMID:25687244

Assessing indoor air quality of school environments: transplanted lichen Pseudovernia furfuracea as a new tool for biomonitoring and bioaccumulation.

PubMed

Protano, Carmela; Owczarek, Malgorzata; Antonucci, Arianna; Guidotti, Maurizio; Vitali, Matteo

2017-07-01

The aim of this research is to evaluate the ability of transplanted lichen Pseudovernia (P). furfuracea to biomonitor and bioaccumulate in urban indoor environments. The elements As, Cd, Cr, Cu, Hg, Ni and Pb and 12 selected polycyclic aromatic hydrocarbons (PAHs) were used to assess P. furfuracea as a biomonitoring tool for the indoor air quality of school environments. To achieve this purpose, lichen samples were exposed for 2 months in the outdoor and indoor environments of five school settings located in urban and rural areas. The results demonstrated that transplanted lichen P. furfuracea is a suitable biomonitoring tool for metals and PAHs in indoor settings and can discriminate between different levels of air pollution related to urbanisation and indoor conditions, such as those characterised by school environments. A transplanted lichen biomonitoring strategy is cost-effective, "green", educational for attending children and less "invasive" than traditional air sampling methods. The feasibility of indoor monitoring by P. furfuracea is a relevant finding and could be a key tool to improve air quality monitoring programmes in school scenarios and thus focus on health prevention interventions for children, who are one of the most susceptible groups in the population.

Enabling rapid behavioral ecotoxicity studies using an integrated lab-on-a-chip systems

NASA Astrophysics Data System (ADS)

Huang, Yushi; Nugegoda, Dayanthi; Wlodkowic, Donald

2015-12-01

Behavioral ecotoxicity tests are gaining an increasing recognition in environmental toxicology. Behavior of sensitive bioindicator species can change rapidly in response to an acute exposure to contaminants and thus has a much higher sensitivity as compared to conventional LC50 mortality tests. Furthermore, behavioral endpoints seems to be very good candidates to develop early-warning biomonitoring systems needed for rapid chemical risk assessment. Behavioral tests are non-invasive, fast, do not harm indicator organisms (behavioural changes are very rapid) and are thus fully compatible with 3R (Replacement - Reduction - Refinement) principle encouraging alternatives to conventional animal testing. These characteristics are essential when designing improved ecotoxicity tests for chemical risk assessment. In this work, we present a pilot development of miniaturized Lab-on-a-Chip (LOC) devices for studying toxin avoidance behaviors of small aquatic crustaceans. As an investigative tool, LOCs represent a new direction that may miniaturize and revolutionize behavioral ecotoxicology. Specifically our innovative microfluidic prototype: (i) enables convening "caging" of specimens for real-time videomicroscopy; (ii) eliminates the evaporative water loss thus providing an opportunity for long-term behavioral studies; (iii) exploits laminar fluid flow under low Reynolds numbers to generate discrete domains and gradients enabling for the first time toxin avoidance studies on small aquatic crustaceans; (iv) integrates off-the-chip mechatronic interfaces and video analysis algorithms for single animal movement analysis. We provide evidence that by merging innovative bioelectronic and biomicrofluidic technologies we can deploy inexpensive and reliable systems for culture, electronic tracking and complex computational analysis of behavior of bioindicator organisms.

Determinants of occupational exposure to metals by gas metal arc welding and risk management measures: a biomonitoring study.

PubMed

Persoons, Renaud; Arnoux, Damien; Monssu, Théodora; Culié, Olivier; Roche, Gaëlle; Duffaud, Béatrice; Chalaye, Denis; Maitre, Anne

2014-12-01

Welding fumes contain various toxic metals including chromium (Cr), nickel (Ni) and manganese (Mn). An assessment of the risk to health of local and systemic exposure to welding fumes requires the assessment of both external and internal doses. The aims of this study were to test the relevance in small and medium sized enterprises of a biomonitoring strategy based on urine spot-samples, to characterize the factors influencing the internal doses of metals in gas metal arc welders and to recommend effective risk management measures. 137 welders were recruited and urinary levels of metals were measured by ICP-MS on post-shift samples collected at the end of the working week. Cr, Ni and Mn mean concentrations (respectively 0.43, 1.69 and 0.27 μg/g creatinine) were well below occupational health guidance values, but still higher than background levels observed in the general population, confirming the absorption of metals generated in welding fumes. Both welding parameters (nature of base metal, welding technique) and working conditions (confinement, welding and grinding durations, mechanical ventilation and welding experience) were predictive of occupational exposure. Our results confirm the interest of biomonitoring for assessing health risks and recommending risk management measures for welders. Copyright © 2014. Published by Elsevier Ireland Ltd.

LIPIDOMICS: A POSSIBLE TOOL FOR THE BIO-MONITORING OF SPECIFIC AIR POLLUTANTS

EPA Science Inventory

Lipidomics examines comprehensive lipid changes in biological systems (whole organisms or individual cells) as biomarkers of effect. Lipidomics is part of the larger field of metabolomics, which examines the specificity and magnitude of perturbations induced by agents, such as a...

Inferring Population Exposure from Biomonitoring Data on Urinary Concentrations (SOT)

EPA Science Inventory

Biomonitoring studies such as the National Health and Nutrition Examination Survey (NHANES) are valuable to exposure assessment both as sources of data to evaluate exposure models and as training sets to develop heuristics for rapid-exposure-assessment tools. However, linking in...

APPLICATIONS OF AUTOMATED BIOMONITORING FOR WATERSHED MANAGEMENT

EPA Science Inventory

Over three decades of progress have been made since John Cairns and his associates first coined a new scientific endeavor known as automated biomonitoring. Implementations have ranged from designs for early warning of toxicity in wastewater discharges using fish as sensors, to co...

Moving Genetic Biomonitoring from a Concept to a Tool

EPA Science Inventory

Molecular genetic techniques like DNA barcoding and environmental DNA have been proposed as tools for aquatic biomonitoring for nearly a decade, but have yet to break through into widespread acceptance. The potential benefits of these methods, such as quicker, cheaper, more detai...

HUMAN MILK BIOMONITORING DATA: INTERPRETATION AND RISK ASSESSMENT ISSUES

EPA Science Inventory

Biomonitoring data can, under certain conditions, be used to describe potential risks to human health (for example, blood lead levels used to determine children's neurodevelopmental risk). At present, there are very few chemical exposures at low levels for which sufficient data ...

A proposal for assessing study quality: Biomonitoring, Environmental Epidemiology, and Short-lived Chemicals (BEES-C) instrument.

PubMed

LaKind, Judy S; Sobus, Jon R; Goodman, Michael; Barr, Dana Boyd; Fürst, Peter; Albertini, Richard J; Arbuckle, Tye E; Schoeters, Greet; Tan, Yu-Mei; Teeguarden, Justin; Tornero-Velez, Rogelio; Weisel, Clifford P

2014-12-01

The quality of exposure assessment is a major determinant of the overall quality of any environmental epidemiology study. The use of biomonitoring as a tool for assessing exposure to ubiquitous chemicals with short physiologic half-lives began relatively recently. These chemicals present several challenges, including their presence in analytical laboratories and sampling equipment, difficulty in establishing temporal order in cross-sectional studies, short- and long-term variability in exposures and biomarker concentrations, and a paucity of information on the number of measurements required for proper exposure classification. To date, the scientific community has not developed a set of systematic guidelines for designing, implementing and interpreting studies of short-lived chemicals that use biomonitoring as the exposure metric or for evaluating the quality of this type of research for WOE assessments or for peer review of grants or publications. We describe key issues that affect epidemiology studies using biomonitoring data on short-lived chemicals and propose a systematic instrument--the Biomonitoring, Environmental Epidemiology, and Short-lived Chemicals (BEES-C) instrument--for evaluating the quality of research proposals and studies that incorporate biomonitoring data on short-lived chemicals. Quality criteria for three areas considered fundamental to the evaluation of epidemiology studies that include biological measurements of short-lived chemicals are described: 1) biomarker selection and measurement, 2) study design and execution, and 3) general epidemiological study design considerations. We recognize that the development of an evaluative tool such as BEES-C is neither simple nor non-controversial. We hope and anticipate that the instrument will initiate further discussion/debate on this topic. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

A proposal for assessing study quality: Biomonitoring, Environmental Epidemiology, and Short-lived Chemicals (BEES-C) instrument

PubMed Central

LaKind, Judy S.; Sobus, Jon R.; Goodman, Michael; Barr, Dana Boyd; Fürst, Peter; Albertini, Richard J.; Arbuckle, Tye E.; Schoeters, Greet; Tan, Yu-Mei; Teeguarden, Justin; Tornero-Velez, Rogelio; Weisel, Clifford P.

2015-01-01

The quality of exposure assessment is a major determinant of the overall quality of any environmental epidemiology study. The use of biomonitoring as a tool for assessing exposure to ubiquitous chemicals with short physiologic half-lives began relatively recently. These chemicals present several challenges, including their presence in analytical laboratories and sampling equipment, difficulty in establishing temporal order in cross-sectional studies, short- and long-term variability in exposures and biomarker concentrations, and a paucity of information on the number of measurements required for proper exposure classification. To date, the scientific community has not developed a set of systematic guidelines for designing, implementing and interpreting studies of short-lived chemicals that use biomonitoring as the exposure metric or for evaluating the quality of this type of research for WOE assessments or for peer review of grants or publications. We describe key issues that affect epidemiology studies using biomonitoring data on short-lived chemicals and propose a systematic instrument – the Biomonitoring, Environmental Epidemiology, and Short-lived Chemicals (BEES-C) instrument – for evaluating the quality of research proposals and studies that incorporate biomonitoring data on short-lived chemicals. Quality criteria for three areas considered fundamental to the evaluation of epidemiology studies that include biological measurements of short-lived chemicals are described: 1) biomarker selection and measurement, 2) study design and execution, and 3) general epidemiological study design considerations. We recognize that the development of an evaluative tool such as BEES-C is neither simple nor non-controversial. We hope and anticipate that the instrument will initiate further discussion/debate on this topic. PMID:25137624

Uso de los Datos de Biomonitoreo para Informar sobre la Evaluacion Infantil (American translation is: USING BIOMONITORING DATA TO INFORM EXPOSURE ASSESSMENT IN CHILDREN)

EPA Science Inventory

Discussing the challenges associated with estimating and interpreting toxicant exposures and health risks from biomonitoring data. This extended abstract was translated in Spanish and published in Acta Toxicologica Argentina.

EVALUATION OF A DAPHNIA BIOMONITOR FOR REAL-TIME DRINKING WATER SOURCE TESTING

EPA Science Inventory

The quality of drinking water sources has come under closer scrutiny in recent years. Issues ranging from ecological to public health, to national security are under consideration. With advances in electronic and computer technology, biomonitors are being developed that can asses...

Reconstruction of Exposure to m-Xylene from Human Biomonitoring Data Using PBPK Modelling, Bayesian Inference, and Markov Chain Monte Carlo Simulation

PubMed Central

McNally, Kevin; Cotton, Richard; Cocker, John; Jones, Kate; Bartels, Mike; Rick, David; Price, Paul; Loizou, George

2012-01-01

There are numerous biomonitoring programs, both recent and ongoing, to evaluate environmental exposure of humans to chemicals. Due to the lack of exposure and kinetic data, the correlation of biomarker levels with exposure concentrations leads to difficulty in utilizing biomonitoring data for biological guidance values. Exposure reconstruction or reverse dosimetry is the retrospective interpretation of external exposure consistent with biomonitoring data. We investigated the integration of physiologically based pharmacokinetic modelling, global sensitivity analysis, Bayesian inference, and Markov chain Monte Carlo simulation to obtain a population estimate of inhalation exposure to m-xylene. We used exhaled breath and venous blood m-xylene and urinary 3-methylhippuric acid measurements from a controlled human volunteer study in order to evaluate the ability of our computational framework to predict known inhalation exposures. We also investigated the importance of model structure and dimensionality with respect to its ability to reconstruct exposure. PMID:22719759

Caddisflies Hydropsyche spp. as biomonitors of trace metal bioavailability thresholds causing disturbance in freshwater stream benthic communities.

PubMed

Awrahman, Zmnako A; Rainbow, Philip S; Smith, Brian D; Khan, Farhan R; Fialkowski, Wojciech

2016-09-01

Demonstration of an ecotoxicological effect of raised toxic metal bioavailabilities on benthic macroinvertebrate communities in contaminated freshwater streams typically requires the labour-intensive identification and quantification of such communities before the application of multivariate statistical analysis. A simpler approach is the use of accumulated trace metal concentrations in a metal-resistant biomonitor to define thresholds that indicate the presence of raised trace metal bioavailabilities causing ecotoxicological responses in populations of more metal-sensitive members of the community. We explore further the hypothesis that concentrations of toxic metals in larvae of species of the caddisfly genus Hydropsyche can be used to predict metal-driven ecotoxicological responses in more metal-sensitive mayflies, especially ephemerellid and heptageniid mayflies, in metal-contaminated rivers. Comparative investigation of two caddisflies, Hydropsyche siltalai and Hydropsyche angustipennis, from metal-contaminated rivers in Cornwall and Upper Silesia, Poland respectively, has provided preliminary evidence that this hypothesis is applicable across caddisfly species and contaminated river systems. Use of a combined toxic unit approach, relying on independent data sets, suggested that copper and probably also arsenic are the drivers of mayfly ecotoxicity in the River Hayle and the Red River in Cornwall, while cadmium, lead and zinc are the toxic agents in the Biala Przemsza River in Poland. This approach has great potential as a simple tool to detect the more subtle effects of mixed trace metal contamination in freshwater systems. An informed choice of suitable biomonitor extends the principle to different freshwater habitats over different ranges of severity of trace metal contamination. Copyright © 2016 Elsevier Ltd. All rights reserved.

[New perspectives in monitoring of exposures to carcinogens].

PubMed

Pavanello, Sofia; Lotti, Marcello

2011-01-01

Biomonitoring occupational and environmental exposures to carcinogens is a common practice and several biomarkers have been developed for risk assessment. However, in particular, because of the lack of prospective studies, the place of these biomarkers within the complex scenario of the gene-environment interactions leading to cancer cannot be defined. New opportunities and suggestions for biomonitoring exposures to carcinogens could derive from exploring the exposome, from the results of genomewide association and omic studies. Based on these premises it is possible to envisage personalized biomonitoring procedures, as those already actuated in nutrition and clinical oncology, allowing a better predictivity of biomarkers in the preventive settings.

Tillandsia usneoides L, a biomonitor in the determination of Ce, La and Sm by neutron activation analysis in an industrial corridor in Central Mexico.

PubMed

Isaac-Olivé, K; Solís, C; Martínez-Carrillo, M A; Andrade, E; López, C; Longoria, L C; Lucho-Constantino, C A; Beltrán-Hernández, R I

2012-04-01

The atmosphere of the Tula Industrial Corridor in Central Mexico is contaminated due to several industries including oil refining while station monitoring in this area are limited. Lanthanides are considered fingerprint of oil refinery activities, and La, Ce and Sm have been previously detected in this area using filters. The suitability of T. usneoides as a biomonitor assessing the La, Ce and Sm concentrations in Particulate Matter is evaluated by NAA. Results of both biomonitor and filters are highly correlated. Copyright © 2012 Elsevier Ltd. All rights reserved.

Organophosphorus and Pyrethroid Insecticide Urinary Metabolite Concentrations in Young Children Living in a Southeastern United States City

EPA Science Inventory

Biomonitoring studies provide valuable information on exposures to chemical contaminants, including pesticides. They can help to identify highly exposed populations and may be used to develop hypotheses about sources and pathways for exposure. A biomonitoring study was conducte...

Communication in a Human biomonitoring study: Focus group work, public engagement and lessons learnt in 17 European countries.

PubMed

Exley, Karen; Cano, Noemi; Aerts, Dominique; Biot, Pierre; Casteleyn, Ludwine; Kolossa-Gehring, Marike; Schwedler, Gerda; Castaño, Argelia; Angerer, Jürgen; Koch, Holger M; Esteban, Marta; Schoeters, Greet; Den Hond, Elly; Horvat, Milena; Bloemen, Louis; Knudsen, Lisbeth E; Joas, Reinhard; Joas, Anke; Dewolf, Marie-Christine; Van de Mieroop, Els; Katsonouri, Andromachi; Hadjipanayis, Adamos; Cerna, Milena; Krskova, Andrea; Becker, Kerstin; Fiddicke, Ulrike; Seiwert, Margarete; Mørck, Thit A; Rudnai, Peter; Kozepesy, Szilvia; Cullen, Elizabeth; Kellegher, Anne; Gutleb, Arno C; Fischer, Marc E; Ligocka, Danuta; Kamińska, Joanna; Namorado, Sónia; Reis, M Fátima; Lupsa, Ioana-Rodica; Gurzau, Anca E; Halzlova, Katarina; Jajcaj, Michal; Mazej, Darja; Tratnik, Janja Snoj; Huetos, Olga; López, Ana; Berglund, Marika; Larsson, Kristin; Sepai, Ovnair

2015-08-01

A communication strategy was developed by The Consortium to Perform Human Biomonitoring on a European Scale (COPHES), as part of its objectives to develop a framework and protocols to enable the collection of comparable human biomonitoring data throughout Europe. The framework and protocols were tested in the pilot study DEMOCOPHES (Demonstration of a study to Coordinate and Perform Human biomonitoring on a European Scale). The aims of the communication strategy were to raise awareness of human biomonitoring, encourage participation in the study and to communicate the study results and their public health significance. It identified the audiences and key messages, documented the procedure for dissemination of results and was updated as the project progressed. A communication plan listed the tools and materials such as press releases, flyers, recruitment letters and information leaflets required for each audience with a time frame for releasing them. Public insight research was used to evaluate the recruitment material, and the feedback was used to improve the documents. Dissemination of results was coordinated in a step by step approach by the participating countries within DEMOCOPHES, taking into account specific national messages according to the needs of each country. Participants received individual results, unless they refused to be informed, along with guidance on what the results meant. The aggregate results and policy recommendations were then communicated to the general public and stakeholders, followed by dissemination at European level. Several lessons were learnt that may assist other future human biomonitoring studies. Recruitment took longer than anticipated and so social scientists, to help with community engagement, should be part of the research team from the start. As a European study, involving multiple countries, additional considerations were needed for the numerous organisations, different languages, cultures, policies and priorities. Therefore, communication documents should be seen as templates with essential information clearly indicated and the option for each country to tailor the material to reflect these differences. Future studies should consider setting up multidisciplinary networks of medical professionals and communication experts, and holding training workshops to discuss the interpretation of results and risk communication. Publicity and wide dissemination of the results helped to raise awareness of human biomonitoring to the general public, policy makers and other key stakeholders. Effective and timely communication, at all stages of a study, is essential if the potential of human biomonitoring research to improve public health is to be realised. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Aquatic biomonitoring of reclaimed water for potable use: the San Diego Health Effects Study.

PubMed

de Peyster, A; Donohoe, R; Slymen, D J; Froines, J R; Olivieri, A W; Eisenberg, D M

1993-05-01

Highly treated reclaimed wastewater was evaluated as a possible supplement to raw water sources required to meet San Diego's growing need for potable water. Biomonitoring experiments employing fathead minnows (Pimephales promelas) were used to compare reclaimed water with the city's current raw water supply. Juvenile fish were exposed in flow-through aquaria in field laboratories located at the reclamation plant (AQUA II) and at a municipal potable water treatment facility (Miramar). Biomonitoring measurements were survival and growth, swimming performance, and trace amounts of 68 base/neutral/acid extractable organics, 27 pesticides, and 27 inorganic chemicals found in fish tissues after exposure. Biomonitoring revealed differences in survival, growth, and swimming performance only after 90- and 180-d exposure. Reclaimed water and raw water were not readily distinguishable in 28-d chemical bioaccumulation tests in terms of organic chemical contaminants in fish tissue except for pesticide levels, which tended to be higher in raw water. Similar inorganic species were found in samples from both waters, although there was greater evidence of bioaccumulation of certain contaminants from raw water. Based on biomonitoring parameters included in these experiments, the use of reclaimed water to supplement raw water supplies would appear to pose no major public health threats. The results of these studies will be combined with additional health effects information before final conclusions are reached about the suitability of reclaimed water for human consumption.

Applying bioethical principles to human biomonitoring.

PubMed

Harrison, Myron

2008-06-05

Bioethical principles are widely used as a normative framework in areas of human research and medical care. In recent years there has been increasing formalization of their use in public health decisions. The "traditional bioethical principles" are applied in this discussion to the important issue human biomonitoring for environmental exposures. They are: (1) Autonomy--Also known as the "respect for humans" principle, people understand their own best interests; (2) Beneficence--"do good" for people; (3) Nonmaleficence--"do no harm"; (4) Justice--fair distribution of benefits and costs (including risks to health) across stakeholders.Some of the points made are: (1) There is not a single generic bioethical analysis applicable to the use of human biomonitoring data, each specific use requires a separate deliberation; (2) Using unidentified, population-based biomonitoring information for risk assessment or population surveillance raises fewer bioethical concerns than personally identified biomonitoring information such as employed in health screening; (3) Companies should proactively apply normative bioethical principles when considering the disposition of products and by-products in the environment and humans; (4) There is a need for more engagement by scholars on the bioethical issues raised by the use of biomarkers of exposure; (5) Though our scientific knowledge of biology will continue to increase, there will always be a role for methods or frameworks to resolve substantive disagreements in the meaning of this data that are matters of belief rather than knowledge.

Applying bioethical principles to human biomonitoring

PubMed Central

Harrison, Myron

2008-01-01

Bioethical principles are widely used as a normative framework in areas of human research and medical care. In recent years there has been increasing formalization of their use in public health decisions. The "traditional bioethical principles" are applied in this discussion to the important issue human biomonitoring for environmental exposures. They are: (1) Autonomy – Also known as the "respect for humans" principle, people understand their own best interests; (2) Beneficence – "do good" for people; (3) Nonmaleficence – "do no harm"; (4) Justice – fair distribution of benefits and costs (including risks to health) across stakeholders. Some of the points made are: (1) There is not a single generic bioethical analysis applicable to the use of human biomonitoring data, each specific use requires a separate deliberation; (2) Using unidentified, population-based biomonitoring information for risk assessment or population surveillance raises fewer bioethical concerns than personally identified biomonitoring information such as employed in health screening; (3) Companies should proactively apply normative bioethical principles when considering the disposition of products and by-products in the environment and humans; (4) There is a need for more engagement by scholars on the bioethical issues raised by the use of biomarkers of exposure; (5) Though our scientific knowledge of biology will continue to increase, there will always be a role for methods or frameworks to resolve substantive disagreements in the meaning of this data that are matters of belief rather than knowledge. PMID:18541074

DEVELOPMENT OF LARGE RIVER BIOASSESSMENT PROTOCOLS (LR-BP) FOR BENTHIC MACROINVERTEBRATES IN EPA REGION 5

EPA Science Inventory

Since passage of the Clean Water Act, government agencies have made extensive use of biomonitoring protocols to report on the quality of wadeable streams and rivers. Non-wadeable systems have been largely overlooked because of

sampling difficulties and a lack of appropriat...

Linking physiological parameters to perturbations in the human exposome: Environmental exposures modify blood pressure and lung function via inflammatory cytokine pathway

EPA Science Inventory

Human biomonitoring is an indispensable tool for establishing the systemic effects from external stressors including environmental pollutants, chemicals from consumer products, and pharmaceuticals. This article uses a combination of new results and meta-data from previous work to...

40 CFR 125.68 - Special conditions for section 301(h) modified permits.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) WATER PROGRAMS CRITERIA AND STANDARDS FOR THE NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM Criteria for Modifying the Secondary Treatment Requirements Under Section 301(h) of the Clean Water Act...: (1) Biomonitoring requirements of § 125.63(b); (2) Water quality requirements of § 125.63(c); (3...

40 CFR 125.68 - Special conditions for section 301(h) modified permits.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (CONTINUED) WATER PROGRAMS CRITERIA AND STANDARDS FOR THE NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM Criteria for Modifying the Secondary Treatment Requirements Under Section 301(h) of the Clean Water Act...: (1) Biomonitoring requirements of § 125.63(b); (2) Water quality requirements of § 125.63(c); (3...

Evaluating Ethanol-based Sample Preservation to Facilitate Use of DNA Barcoding in Routine Freshwater Biomonitoring Programs Using Benthic Macroinvertebrates

EPA Science Inventory

Molecular methods, such as DNA barcoding, have the potential in enhance biomonitoring programs worldwide. Altering routinely used sample preservation methods to protect DNA from degradation may pose a potential impediment to application of DNA barcoding and metagenomics for biom...

A Proposal for Assessing Study Quality: Biomonitoring, Environmental Epidemiology, and Short-Lived Chemicals (BEES-C) Instrument

EPA Science Inventory

The quality of exposure assessment is a major determinant of the overall quality of any environmental epidemiology study. The use of biomonitoring as a tool for assessing exposure to ubiquitous chemicals with short physiologic half-lives began relatively recently. These chemicals...

Biomonitoring: Uses and Considerations for Assessing Non-Occupational Human Exposure to Pesticides

EPA Science Inventory

Biomonitoring is an important tool that can be used to evaluate human exposure to pesticides by measuring the levels of pesticides, pesticide metabolites, or altered biological structures or functions in biological specimens or tissues (Barr et al., 2005b; Needham et al., 2005, 2...

A New Method for Generating Distribution of Biomonitoring Equivalents to Support Exposure Assessment and Prioritization

EPA Science Inventory

Biomonitoring data are now available for hundreds of chemicals through state and national health surveys. Exposure guidance values also exist for many of these chemicals. Several methods are frequently used to evaluate biomarker data with respect to a guidance value. The “biomoni...

Assessing the Quantitative Relationships between Preschool Children's Exposures to Bisphenol A by Route and Urinary Biomonitoring

EPA Science Inventory

Limited published information exists on young children’s exposures to bisphenol A (BPA) in the United States using urinary biomonitoring. In a previous project, we quantified the aggregate exposures of 257 preschool children to BPA in environmental and personal media over 48-h pe...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kemp, H. T.

The Inventory has resulted in establishment of a series of data bases containing biological monitoring information of varying types, namely, directory of investigators, record of projects received from mail questionnaire, detailed description of selected biomonitoring projects, and bibliographic citations supporting the projects received. This report contains detailed descriptions of selected biomonitoring projects organized on a state-by-state basis and with appropriate indices.

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

NASA Astrophysics Data System (ADS)

Wu, Huifeng; Ji, Chenglong; Wang, Qing; Liu, Xiaoli; Zhao, Jianmin; Feng, Jianghua

2013-01-01

The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate significant biological differences in gills of white and zebra clams based on metabolic profiles and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profiles, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

Biomonitoring persistent organic pollutants in the atmosphere with mosses: performance and application.

PubMed

Wu, Qimei; Wang, Xin; Zhou, Qixing

2014-05-01

Persistent organic pollutants (POPs) have aroused environmentalists and public concerns due to their toxicity, bioaccumulation and persistency in the environment. However, monitoring atmospheric POPs using conventional instrumental methods is difficult and expensive, and POP levels in air samples represent an instantaneous value at a sampling time. Biomonitoring methods can overcome this limitation, because biomonitors can accumulate POPs, serve as long-term integrators of POPs and provide reliable information to assess the impact of pollutants on the biota and various ecosystems. Recently, mosses are increasingly employed to monitor atmospheric POPs. Mosses have been applied to indicate POP pollution levels in the remote continent of Antarctica, trace distribution of POPs in the vicinity of pollution sources, describe the spatial patterns at the regional scale, and monitor the changes in the pollution intensity along time. In the future, many aspects need to be improved and strengthened: (i) the relationship between the concentrations of POPs in mosses and in the atmosphere (different size particulates and vapor phases); and (ii) the application of biomonitoring with mosses in human health studies. Copyright © 2014 Elsevier Ltd. All rights reserved.

The novel approach to the biomonitor survey using one- and two-dimensional Kohonen networks.

PubMed

Deljanin, Isidora; Antanasijević, Davor; Urošević, Mira Aničić; Tomašević, Milica; Perić-Grujić, Aleksandra; Ristić, Mirjana

2015-10-01

To compare the applicability of the leaves of horse chestnut (Aesculus hippocastanum) and linden (Tilia spp.) as biomonitors of trace element concentrations, a coupled approach of one- and two-dimensional Kohonen networks was applied for the first time. The self-organizing networks (SONs) and the self-organizing maps (SOMs) were applied on the database obtained for the element accumulation (Cr, Fe, Ni, Cu, Zn, Pb, V, As, Cd) and the SOM for the Pb isotopes in the leaves for a multiyear period (2002-2006). A. hippocastanum seems to be a more appropriate biomonitor since it showed more consistent results in the analysis of trace elements and Pb isotopes. The SOM proved to be a suitable and sensitive tool for assessing differences in trace element concentrations and for the Pb isotopic composition in leaves of different species. In addition, the SON provided more clear data on seasonal and temporal accumulation of trace elements in the leaves and could be recommended complementary to the SOM analysis of trace elements in biomonitoring studies.

Traffic contribution to air pollution in urban street canyons: Integrated application of the OSPM, moss biomonitoring and spectral analysis

NASA Astrophysics Data System (ADS)

Lazić, Lazar; Urošević, Mira Aničić; Mijić, Zoran; Vuković, Gordana; Ilić, Luka

2016-09-01

To investigate the air pollutant distribution within the ambient of urban street canyon, Operational Street Pollution Model (OSPM) was used to predict hourly content of NOX, NO, NO2, O3, CO, BNZ and PM10. The study was performed in five street canyons in Belgrade (Serbia) during 10-week summer period. The model receptors were located on each side of street canyons at 4 m, 8 m and 16 m height. To monitor airborne trace element content, the moss bag biomonitors were simultaneously exposed with the model receptors at two heights-4 m and 16 m. The results of both methods, modelling and biomonitoring, showed significantly decreasing trend of the air pollutants with height. The results indirectly demonstrate that biomonitoring, i.e., moss bag technique could be a valuable tool to verify model performance. In addition, spectral analysis was applied to investigate weekly variation of the daily background and modelled data set. Typical periodicities and weekend effect, caused by anthropogenic influences, have been identified.

The use of biomonitoring data in exposure and human health risk assessment: benzene case study.

PubMed

Arnold, Scott M; Angerer, Juergen; Boogaard, Peter J; Hughes, Michael F; O'Lone, Raegan B; Robison, Steven H; Schnatter, A Robert

2013-02-01

Abstract A framework of "Common Criteria" (i.e. a series of questions) has been developed to inform the use and evaluation of biomonitoring data in the context of human exposure and risk assessment. The data-rich chemical benzene was selected for use in a case study to assess whether refinement of the Common Criteria framework was necessary, and to gain additional perspective on approaches for integrating biomonitoring data into a risk-based context. The available data for benzene satisfied most of the Common Criteria and allowed for a risk-based evaluation of the benzene biomonitoring data. In general, biomarker (blood benzene, urinary benzene and urinary S-phenylmercapturic acid) central tendency (i.e. mean, median and geometric mean) concentrations for non-smokers are at or below the predicted blood or urine concentrations that would correspond to exposure at the US Environmental Protection Agency reference concentration (30 µg/m(3)), but greater than blood or urine concentrations relating to the air concentration at the 1 × 10(-5) excess cancer risk (2.9 µg/m(3)). Smokers clearly have higher levels of benzene exposure, and biomarker levels of benzene for non-smokers are generally consistent with ambient air monitoring results. While some biomarkers of benzene are specific indicators of exposure, the interpretation of benzene biomonitoring levels in a health-risk context are complicated by issues associated with short half-lives and gaps in knowledge regarding the relationship between the biomarkers and subsequent toxic effects.

Response characteristics of an aquatic biomonitor used for rapid toxicity detection.

PubMed

van der Schalie, W H; Shedd, T R; Widder, M W; Brennan, L M

2004-01-01

The response characteristics of an aquatic biomonitor that detects toxicity by monitoring changes in bluegill (Lepomis macrochirus Rafinesque) ventilatory and movement patterns were evaluated in single chemical laboratory studies at concentrations near the 96-h LC(50) concentration and at the EILATox-Oregon Workshop in sequential tests of multiple unknown samples. Baseline data collected prior to exposure allows each fish to serve as its own control. When at least 70% of exposed fish exhibit ventilatory or movement parameters significantly different from baseline observations, a group alarm is declared. In the laboratory studies, the aquatic biomonitor responded to the majority of chemicals at the 96-h lc(50) within an hour or less, although substantially higher response times were found for malathion and pentachlorophenol. Workshop tests of single chemical concentrations presented as blind samples were consistent with the laboratory test results. There were no alarms under control conditions in any test. Although data are limited, the aquatic biomonitor appears to respond more rapidly to chemicals causing membrane irritation, narcosis or polar narcosis than to acetylcholinesterase inhibitors or oxidative phosphorylation uncouplers. All four monitored parameters (ventilatory rate, cough rate, ventilatory depth and movement) contributed to identification of first alarms at acutely toxic levels. Understanding these response patterns can be useful in data interpretation for biomonitor applications such as surface water monitoring for watershed protection, wastewater treatment plant effluent monitoring or source water monitoring for drinking water protection. Copyright (c) 2004 John Wiley & Sons, Ltd.

Deriving Biomonitoring Equivalents for selected E- and P-series glycol ethers for public health risk assessment.

PubMed

Poet, Torka; Ball, Nicholas; Hays, Sean M

2016-01-01

Glycol ethers are a widely used class of solvents that may lead to both workplace and general population exposures. Biomonitoring studies are available that have quantified glycol ethers or their metabolites in blood and/or urine amongst exposed populations. These biomonitoring levels indicate exposures to the glycol ethers, but do not by themselves indicate a health hazard risk. Biomonitoring Equivalents (BEs) have been created to provide the ability to interpret human biomonitoring data in a public health risk context. The BE is defined as the concentration of a chemical or metabolite in a biological fluid (blood or urine) that is consistent with exposures at a regulatory derived safe exposure limit, such as a tolerable daily intake (TDI). In this exercise, we derived BEs for general population exposures for selected E- and P-series glycol ethers based on their respective derived no effect levels (DNELs). Selected DNELs have been derived as part of respective Registration, Evaluation, Authorisation and Regulation of Chemicals (REACh) regulation dossiers in the EU. The BEs derived here are unique in the sense that they are the first BEs derived for urinary excretion of compounds following inhalation exposures. The urinary mass excretion fractions (Fue) of the acetic acid metabolites for the E-series GEs range from approximately 0.2 to 0.7. The Fues for the excretion of the parent P-series GEs range from approximately 0.1 to 0.2, with the exception of propylene glycol methyl ether and its acetate (Fue = 0.004). Despite the narrow range of Fues, the BEs exhibit a larger range, resulting from the larger range in DNELs across GEs. The BEs derived here can be used to interpret human biomonitoring data for inhalation exposures to GEs amongst the general population. Copyright © 2015 Elsevier GmbH. All rights reserved.

Risk communication and human biomonitoring: which practical lessons from the Belgian experience are of use for the EU perspective?

PubMed

Keune, Hans; Morrens, Bert; Loots, Ilse

2008-06-05

In order to investigate and monitor environmental health in Flanders (the Dutch speaking part of Belgium), the Flemish government funded the Centre of Expertise for Environment and Health, which started a human biomonitoring campaign in 2001. In addition to environmental health experts measuring environmental pollutants and health effects in human beings, social scientific experts at the Centre focus on risk communication associated with the human biomonitoring campaign. In the literature about risk communication an evolution can be traced from traditional, one-way communication, restricted to the dissemination of information from experts to the public, to more modern, two-way risk communication, with a focus on participation and cooperation between scientists, policy-makers and the public.Within the Centre of Expertise for Environment and Health this discourse was first translated into some general principles and guidelines for external communication, at a 'Ten Commandments level'. These principles needed to be incorporated in the day-to-day practice of human biomonitoring research. The social scientific experts at the Centre developed a combined risk communication strategy. On the one hand the strategy consists of traditional risk communication for external communication purposes, for example information meetings and digital newsletters. On the other hand it consists of a step by step approach of incorporating more modern risk communication, for example a risk perception questionnaire, dialogical experiments for involving local stakeholders, and an action-plan for interpreting results for policy making. With a parallel strategy of traditional and modern communication, of external and internal reflection, and through different social scientific projects, the Flemish Centre of Expertise of Environment and Health incorporates risk communication in the day-to-day practice of human biomonitoring research. A direct and continuous involvement of the social scientist, an openness between all colleagues involved, and the awareness of a fine balance between quality and practicability are important success factors. These lessons may be helpful and inspirational for a European human biomonitoring project.

An overview of human biomonitoring of environmental chemicals in the Canadian Health Measures Survey: 2007-2019.

PubMed

Haines, Douglas A; Saravanabhavan, Gurusankar; Werry, Kate; Khoury, Cheryl

2017-03-01

Human biomonitoring (HBM) is used to indicate and quantify exposure by measuring environmental chemicals, their metabolites or reaction products in biological specimens. The biomonitoring component of the Canadian Health Measures Survey (CHMS) is the most comprehensive initiative providing general population HBM data in Canada. The CHMS is an ongoing cross-sectional direct measures survey implemented in 2-year cycles. It provides nationally-representative data on health, nutritional status, environmental exposures, and related risks and protective characteristics. The survey follows a robust planning, design and sampling protocol as well as a comprehensive quality assurance and quality control regime implemented for all aspect of the survey to ensure the validity of the HBM results. HBM blood and urine data are available for CHMS cycles 1 (2007-2009), 2 (2009-2011) and 3 (2012-2013). Field collection has been completed for cycle 4 (2014-2015), with cycle 5 (2016-2017) in progress and cycle 6 planning (2018-2019) being finalized. Biomonitoring results for 279 chemicals are expected over the six cycles of the CHMS (220 in individual blood, urine or hair samples, and 59 in pooled serum samples). The chemicals include metals and trace elements, polychlorinated biphenyls (PCBs), organochlorines, flame retardants, perfluoroalkyl substances, volatile organic compounds (VOCs) and metabolites, environmental phenols, triclocarban, acrylamide, pesticides (e.g., triazines, carbamates, organophosphates, phenoxy, pyrethroids) and/or their metabolites, chlorophenols, polycyclic aromatic hydrocarbon (PAH) metabolites, phthalates and alternate plasticizer metabolites, and tobacco biomarkers. Approximately one half of the chemicals measured in individual blood and urine samples over the first three cycles were detected in more than 60% of samples. CHMS biomonitoring data have been used to establish baseline HBM concentrations in Canadians; inform public health, regulatory risk assessment and management decisions; and fulfil national and international reporting requirements. Concurrent efforts are underway in Canada to develop statistically- and risk-based concepts and tools to interpret biomonitoring data. Crown Copyright © 2016. Published by Elsevier GmbH. All rights reserved.

Biomonitoring of boron: Development and characterization of a simple, reliable and quality controlled biomonitoring method.

PubMed

Michalke, Bernhard

2017-03-01

Boron exposure is of interest and concern from an occupational point of view. Usual daily boron intake is related to boron blood plasma concentration <1mg/L and to <3mg/L in urine, but after exposure urine concentrations are quickly elevated. Reliable boron biomonitoring, typically in urine, thus is mandatory for occupational health control institutions. This paper reports on the development of a simple, fast and reliable boron determination procedure based on inductively coupled plasma - optical emission spectrometry (ICP-OES). Major aims for this method were simplicity in sample preparation, low risk for artifacts and interferences, high precision and accuracy, possibly low costs, including lower costs for element selective detection, short total analysis time and suitability for occupational health laboratories. Precision data (serial or day-to-day) from urine and doped urine were very good: <1.5 or <2%. Accuracy was calculated from analysis of a certified reference material (ERM-CD 281), as 99% or according to recoveries of doped concentrations ranging from 102 to 109% recovery. For cross-checking ICP-OES determinations, samples were analyzed also by quadrupole ICP-qMS and by sectorfield ICP-sf-MS at low and medium resolution. Both systems confirmed ICP-OES measurements when using 11 B for quantification. Determinations based on 10 B however showed some bias, except with ICP-sf-MS at medium resolution. The observed elevated signals are discussed with respect to the known Ne ++ interference (as an impurity in Ar), which is not separated in low resolving quadrupole ICP-MS systems or ICP-sf-MS at low resolution. Copyright © 2016 Elsevier GmbH. All rights reserved.

Towards a global barcode library for Lymantria (Lepidoptera: Lymantriinae) tussock moths of biosecurity concern

Treesearch

Jeremy R. deWaard; Andrew Mitchell; Melody A. Keena; David Gopurenko; Laura M. Boykin; Karen F. Armstrong; Michael G. Pogue; Joao Lima; Robin Floyd; Robert H. Hanner; Leland M. Humble

2010-01-01

This study demonstrates the efficacy of DNA barcodes for diagnosing species of Lymantria and reinforces the view that the approach is an under-utilized resource with substantial potential for biosecurity and surveillance. Biomonitoring agencies currently employing the NB restriction digest system would gather more information by transitioning to the...

Hg localisation in Tillandsia usneoides L. (Bromeliaceae), an atmospheric biomonitor

NASA Astrophysics Data System (ADS)

Amado Filho, G. M.; Andrade, L. R.; Farina, M.; Malm, O.

The Spanish moss, Tillandsia usneoides, has been applied as an atmospheric biomonitor of Hg contamination, although the mechanism of metal plant accumulation has not been understood until now. In the present work, analytical scanning electron microscopy (SEM) was used to localize Hg in T. usneoides exposed to a Hg-air-contaminated area during 15 days. After this period, Hg was determined by the flow injection mercury system, and plants were prepared for SEM observation and energy-dispersive X-ray analysis. A concentration of 2702±318 μg Hg g -1 was determined in exposed plants. The presented microanalytical results demonstrated that Hg was partly associated with atmospheric particles deposited upon the plant surface, but it was highly absorbed by the scales, stem and leaves surfaces and less absorbed by epidermal cells of T. usneoides. No Hg was detected in mesophyll parenchyma or in vascular system cells. The great surface adsorption area provided by the scales, in addition to the characteristics of T. usneoides morphology, especially of the node region, are suggested to confer the great capability of T. usneoides in Hg holding.

DNA ARRAYS TO MONITOR GENE EXPRESSION IN RAT BLOOD AND UTERUS FOLLOWING 17-BETA-ESTRADIOL EXPOSURE: BIOMONITORING ENVIRONMENTAL EFFECTS USING SURROGATE TISSUES

EPA Science Inventory

DNA arrays to monitor gene expression in rat blood and uterus following 17-b-estradiol exposure - biomonitoring environmental effects using surrogate tissues
John C. Rockett, Robert J. Kavlock, Christy R. Lambright, Louise G. Parks, Judith E. Schmid, Vickie S. Wilson, Carmen W...

DNA ARRAYS TO MONITOR GENE EXPRESSION IN RAT BLOOD AND UTERUS FOLLOWING 17BETA-ESTRADIOL EXPOSURE: BIOMONITORING ENVIRONMENTAL EFFECTS USING SURROGATE TISSUES

EPA Science Inventory

We propose that gene expression changes in accessible tissues such as blood often reflect those in inaccessible tissues, thus offering a convenient biomonitoring method to provide insight into the effects of environmental toxicants on such tissues. In this pilot study, gene expre...

Biomonitoring Breast Milk Polybrominated Diphenyl Ethers as a Function of Environment, Dietary Intake, and Demographics in New Hampshire

USDA-ARS?s Scientific Manuscript database

Breast milk is a valuable biological specimen for biomonitoring lipid-soluble polybrominated diphenyl ethers (PBDEs). The purpose of this study was to determine the levels of PBDEs in breast milk from New Hampshire and to examine potential relationships between PBDE levels in breast milk and stage o...

Green garden snail, Cantareus apertus, as biomonitor and sentinel for integrative metal pollution assessment in roadside soils.

PubMed

Mleiki, Anwar; Marigómez, Ionan; El Menif, Najoua Trigui

2017-11-01

The present investigation was conceived to study, in a small scale field study, the potential of the green garden snail, Cantareus apertus, as biomonitor and sentinel for integrative metal pollution assessment in soils. For this purpose, we investigated the association between the trace metal (Cd, Pb, As, Fe, Cr, Cu, Ni, and Zn) concentrations in soil, plants (Trifolium repens), and C. apertus depending on the distance (20, 150, and 700 m) from a main roadside in Tunisia as well as between metal concentrations and biomarkers of oxidative stress, oxidative damage, and neurotoxicity in C. apertus. Results revealed a clear association between the concentration of metals such as Ni, Cu, and Zn in snail digestive gland, both amongst them and with oxidative stress and neurotoxicity biomarkers recorded in the same organ. Interestingly, Ni, Pb, and Zn occurred at the highest concentration in soil, plant, and snails and the association appeared related to the immediacy of the roadside and the concentration of these three metals tended to decrease with distance from the roadside in the soil-plant-snail system. Conversely, Cd and Cu were bioaccumulated in plants and snails but their concentrations in soil were not high and did not show a decline in concentration with distance from the roadside. After PCA analysis, PC-01 (56% of the variance) represented metal bioaccumulation and associated toxic effects in snails in the presence of high levels of metal pollution (nearby the roadside) while PC-02 (35% of the variance) represented stress induced by moderate levels of metal pollution (at intermediate distances from the roadside). The four studied sites were clearly discriminated one from each other, depending on how they are affected by traffic pollution. In summary, this field study reveals that (a) C. apertus can be used as biomonitor for metal pollution in roadside soils and as sentinel for pollution effects assessment based on biochemical biomarkers; and (b) that oxidative stress and neurotoxicity biomarkers endow with a powerful biological tool for metal pollution biomonitoring in soils, especially in combination with chemical analysis of the soil-plant-snail transfer system. Moreover, this study provides some baseline data for future impact assessments concerning trace metal pollution in Tunisia.

Computational Toxicology of Chloroform: Reverse Dosimetry Using Bayesian Inference, Markov Chain Monte Carlo Simulation, and Human Biomonitoring Data

PubMed Central

Lyons, Michael A.; Yang, Raymond S.H.; Mayeno, Arthur N.; Reisfeld, Brad

2008-01-01

Background One problem of interpreting population-based biomonitoring data is the reconstruction of corresponding external exposure in cases where no such data are available. Objectives We demonstrate the use of a computational framework that integrates physiologically based pharmacokinetic (PBPK) modeling, Bayesian inference, and Markov chain Monte Carlo simulation to obtain a population estimate of environmental chloroform source concentrations consistent with human biomonitoring data. The biomonitoring data consist of chloroform blood concentrations measured as part of the Third National Health and Nutrition Examination Survey (NHANES III), and for which no corresponding exposure data were collected. Methods We used a combined PBPK and shower exposure model to consider several routes and sources of exposure: ingestion of tap water, inhalation of ambient household air, and inhalation and dermal absorption while showering. We determined posterior distributions for chloroform concentration in tap water and ambient household air using U.S. Environmental Protection Agency Total Exposure Assessment Methodology (TEAM) data as prior distributions for the Bayesian analysis. Results Posterior distributions for exposure indicate that 95% of the population represented by the NHANES III data had likely chloroform exposures ≤ 67 μg/L in tap water and ≤ 0.02 μg/L in ambient household air. Conclusions Our results demonstrate the application of computer simulation to aid in the interpretation of human biomonitoring data in the context of the exposure–health evaluation–risk assessment continuum. These results should be considered as a demonstration of the method and can be improved with the addition of more detailed data. PMID:18709138

The use of biomonitoring data in exposure and human health risk assessment: benzene case study

PubMed Central

Angerer, Juergen; Boogaard, Peter J.; Hughes, Michael F.; O’Lone, Raegan B.; Robison, Steven H.; Robert Schnatter, A.

2013-01-01

A framework of “Common Criteria” (i.e. a series of questions) has been developed to inform the use and evaluation of biomonitoring data in the context of human exposure and risk assessment. The data-rich chemical benzene was selected for use in a case study to assess whether refinement of the Common Criteria framework was necessary, and to gain additional perspective on approaches for integrating biomonitoring data into a risk-based context. The available data for benzene satisfied most of the Common Criteria and allowed for a risk-based evaluation of the benzene biomonitoring data. In general, biomarker (blood benzene, urinary benzene and urinary S-phenylmercapturic acid) central tendency (i.e. mean, median and geometric mean) concentrations for non-smokers are at or below the predicted blood or urine concentrations that would correspond to exposure at the US Environmental Protection Agency reference concentration (30 µg/m3), but greater than blood or urine concentrations relating to the air concentration at the 1 × 10−5 excess cancer risk (2.9 µg/m3). Smokers clearly have higher levels of benzene exposure, and biomarker levels of benzene for non-smokers are generally consistent with ambient air monitoring results. While some biomarkers of benzene are specific indicators of exposure, the interpretation of benzene biomonitoring levels in a health-risk context are complicated by issues associated with short half-lives and gaps in knowledge regarding the relationship between the biomarkers and subsequent toxic effects. PMID:23346981

Alpine vegetation communities and the alpine-treeline ecotone boundary in New England as biomonitors for climate change

Treesearch

Kenneth D. Kimball; Douglas M. Weihrauch

2000-01-01

This study mapped and analyzed the alpine-treeline ecotone (ATE) boundary and alpine plant communities on the Presidential Range, New Hampshire and Mount Katahdin, Maine. These are sensitive biomonitoring parameters for plant community responses to climatic change. The ATE boundary spans a considerable elevational range, suggesting that shorter growing seasons with...

PAH detection in Quercus robur leaves and Pinus pinaster needles: A fast method for biomonitoring purpose.

PubMed

De Nicola, F; Concha Graña, E; Aboal, J R; Carballeira, A; Fernández, J Á; López Mahía, P; Prada Rodríguez, D; Muniategui Lorenzo, S

2016-06-01

Due to the complexity and heterogeneity of plant matrices, new procedure should be standardized for each single biomonitor. Thus, here is described a matrix solid-phase dispersion extraction method, previously used for moss samples, improved and modified for the analyses of PAHs in Quercus robur leaves and Pinus pinaster needles, species widely used in biomonitoring studies across Europe. The improvements compared to the previous procedure are the use of Florisil added with further clean-up sorbents, 10% deactivated silica for pine needles and PSA for oak leaves, being these matrices rich in interfering compounds, as shown by the gas chromatography-mass spectrometry analyses acquired in full scan mode. Good trueness, with values in the range 90-120% for the most of compounds, high precision (intermediate precision between 2% and 12%) and good sensitivity using only 250mg of samples (limits of quantification lower than 3 and 1.5ngg(-1), respectively for pine and oak) were achieved by the selected procedures. These methods proved to be reliable for PAH analyses and, having advantage of fastness, can be used in biomonitoring studies of PAH air contamination. Copyright © 2016 Elsevier B.V. All rights reserved.

Identifying Chemical Groups for Biomonitoring

PubMed Central

Krowech, Gail; Hoover, Sara; Plummer, Laurel; Sandy, Martha; Zeise, Lauren; Solomon, Gina

2016-01-01

Summary: Regulatory agencies face daunting challenges identifying emerging chemical hazards because of the large number of chemicals in commerce and limited data on exposure and toxicology. Evaluating one chemical at a time is inefficient and can lead to replacement with uncharacterized chemicals or chemicals with structural features already linked to toxicity. The Office of Environmental Health Hazard Assessment (OEHHA) has developed a process for constructing and assessing chemical groups for potential biomonitoring in California. We screen for chemicals with significant exposure potential and propose possible chemical groups, based on structure and function. To support formal consideration of these groups by Biomonitoring California’s Scientific Guidance Panel, we conduct a detailed review of exposure and toxicity data and examine the likelihood of detection in biological samples. To date, 12 chemical groups have been constructed and added to the pool of chemicals that can be selected for Biomonitoring California studies, including p,p´-bisphenols, brominated and chlorinated organic compounds used as flame retardants, non-halogenated aromatic phosphates, and synthetic polycyclic musks. Evaluating chemical groups, rather than individual chemicals, is an efficient way to respond to shifts in chemical use and the emergence of new chemicals. This strategy can enable earlier identification of important chemicals for monitoring and intervention. PMID:27905275

Biomonitoring of trace elements in urine samples of children from a coal-mining region.

PubMed

Santos, Marina Dos; Flores Soares, Maria Cristina; Martins Baisch, Paulo Roberto; Muccillo Baisch, Ana Luíza; Rodrigues da Silva Júnior, Flavio Manoel

2018-04-01

Biomonitoring through urine samples is important for evaluating environmental exposure, since urine is the main form of excretion for most chemical elements. Children are considered more vulnerable to adverse environmental conditions, especially children in developing countries. This study aimed to biomonitor trace elements in urine samples in children from a coal-mining region in the extreme south of Brazil. A cross-sectional study was conducted on 96 children between 6 and 11 years of age. Socioeconomic data and urine samples were collected to estimate the concentration of iron, zinc, selenium, lead, and cadmium. The prevalence of metals above the reference values was 52.0% for Se, followed by 15.6% for Zn. The data point toward a vulnerability to adverse environmental conditions in these children. Although the concentrations of the elements did not reveal intoxication cases, biomonitoring should be carried out continuously in order to assess exposure to metals and ensure the health of the population. This article provides data that help determine natural levels of metallic elements in children, specifically in South America, which have not yet been established. Copyright © 2018 Elsevier Ltd. All rights reserved.

Biomonitoring of perfluorinated compounds in a drop of blood.

PubMed

Mao, Pan; Wang, Daojing

2015-06-02

Biomonitoring of pollutants and their metabolites and derivatives using biofluids provides new opportunities for spatiotemporal assessment of human risks to environmental exposures. Perfluorinated compounds (PFCs) have been used widely in industry and pose significant environmental concerns due to their stability and bioaccumulation in humans and animals. However, current methods for extraction and measurement of PFCs require relatively large volumes (over one hundred microliters) of blood samples, and therefore, are not suitable for frequent blood sampling and longitudinal biomonitoring of PFCs. We have developed a new microassay, enabled by our silicon microfluidic chip platform, for analyzing PFCs in small volumes (less than five microliters) of blood. Our assay integrates on-chip solid-phase extraction (SPE) with online nanoflow liquid chromatography-electrospray ionization-mass spectrometry (nanoLC-ESI-MS) detection. We demonstrated high sample recovery, excellent interday and intraday accuracy and precision, and a limit of detection down to 50 femtogram of PFCs, in one microliter of human plasma. We validated our assay performance using pooled human plasma and NIST SRM 1950 samples. Our microfluidic chip-based assay may enable frequent longitudinal biomonitoring of PFCs and other environmental toxins using a finger prick of blood, thereby providing new insights into their bioaccumulation, bioavailability, and toxicity.

Assessment of metal contamination in the Hun River, China, and evaluation of the fish Zacco platypus and the snail Radix swinhoei as potential biomonitors.

PubMed

Wu, Xing; Wang, Shaofeng; Chen, Hongxing; Jiang, Zhiqiang; Chen, Hongwei; Gao, Mi; Bi, Ran; Klerks, Paul L; Wang, He; Luo, Yongju; Xie, Lingtian

2017-03-01

The Hun River is a major tributary of the Liao River in the northeast area of China and provides drinking water for 23 million local residents. This study was designed to assess the severity of metal contamination in the Hun River and the potential use of indigenous organisms (the fish Zacco platypus and the snail Radix swinhoei) as biomonitors of metal contamination. Water, sediment, and the native fish and snails were collected at four sampling sites that differed in their physicochemical characteristics and their contamination levels. The samples were analyzed for Cd, Cr, Cu, Ni, Pb, and Zn by ICP-MS. The results showed that although the overall potential ecological risks of the metals were low at our sampling sites, Cd posed a noteworthy ecological risk. Strong correlations were obtained between Cd concentrations in the organisms and in the environment. The results indicated that Z. platypus and R. swinhoei can be useful biomonitoring species for assessing Cd contamination. Biomonitoring with the snail may be most effective when focused on the gonad/digestive tissue (because of the high metal accumulation there), but further work is needed to confirm this.

PCBs and DDE in Tree Swallow (Tachycineta bicolor) Eggs and Nestlings from an Estuarine PCB Superfund Site, New Bedford Harbor, MA, U.S.A.

EPA Science Inventory

While breeding tree swallows (Tachycineta bicolor) have been used as biomonitors for freshwater sites, we report the first use of this species to assess the transfer of breeding ground contaminants from an estuarine system. Eggs and nestlings were collected from nest boxes locat...

Metabolism and Biomarkers of Heterocyclic Aromatic Amines in Molecular Epidemiology Studies: Lessons Learned from Aromatic Amines

PubMed Central

2011-01-01

Aromatic amines and heterocyclic aromatic amines (HAAs) are structurally related classes of carcinogens that are formed during the combustion of tobacco or during the high-temperature cooking of meats. Both classes of procarcinogens undergo metabolic activation by N-hydroxylation of the exocyclic amine group, to produce a common proposed intermediate, the arylnitrenium ion, which is the critical metabolite implicated in toxicity and DNA damage. However, the biochemistry and chemical properties of these compounds are distinct and different biomarkers of aromatic amines and HAAs have been developed for human biomonitoring studies. Hemoglobin adducts have been extensively used as biomarkers to monitor occupational and environmental exposures to a number of aromatic amines; however, HAAs do not form hemoglobin adducts at appreciable levels and other biomarkers have been sought. A number of epidemiologic studies that have investigated dietary consumption of well-done meat in relation to various tumor sites reported a positive association between cancer risk and well-done meat consumption, although some studies have shown no associations between well-done meat and cancer risk. A major limiting factor in most epidemiological studies is the uncertainty in quantitative estimates of chronic exposure to HAAs and, thus, the association of HAAs formed in cooked meat and cancer risk has been difficult to establish. There is a critical need to establish long-term biomarkers of HAAs that can be implemented in molecular epidemioIogy studies. In this review article, we highlight and contrast the biochemistry of several prototypical carcinogenic aromatic amines and HAAs to which humans are chronically exposed. The biochemical properties and the impact of polymorphisms of the major xenobiotic-metabolizing enzymes on the biological effects of these chemicals are examined. Lastly, the analytical approaches that have been successfully employed to biomonitor aromatic amines and HAAs, and emerging biomarkers of HAAs that may be implemented in molecular epidemiology studies are discussed. PMID:21688801

Initial Technology Assessment for the Environmental Sentinel Biomonitor (ESB) System

DTIC Science & Technology

2006-08-01

McFarlane-Abdulla E, Andersson M, Barile FA, et al. 1996. MEIC evaluation of acute systemic toxicity Part II. In vitro results from 68 toxicity assays used...to test the first 30 reference chemicals and a comparative cytotoxicity analysis. A TLA 24:273-311. Clemedson C, Andersson M, Aoki Y, Barile FA, et al...Clemedson C, McFarlane-Abdulla E, Andersson M, Barile FA, et al. 1996. MEIC evaluation of acute systemic toxicity Part I. Methodology of 68 in vitro

Ethics in biomonitoring for occupational health.

PubMed

Manno, M; Sito, F; Licciardi, L

2014-12-01

Biological monitoring, i.e., the use of biomarkers for the measurement of systemic human exposure, effects and susceptibility to chemicals has increased considerably in recent years. Biomonitoring techniques, originally limited to a few metals and other chemicals in the workplace, are currently applied to a large number of exposure situations and have become a useful tool for occupational and environmental health risk assessment. Almost any biomonitoring program, however, entails a number of relevant ethical issues, which concern all the phases of the entire process, from the selection of the biomarker to the study design, from the collection, storage and analysis of the biological sample to the interpretation, communication and management of the results, from the (truly?) informed consent of the worker to the independence and autonomy of the occupational health professional. These issues require a balanced assessment of the interests and responsibilities of all the parties, the worker primarily, but also the employer, the occupational health professional, the health authorities and, for research studies on new biomarkers, also the scientists involved. Ideally, decisions of ethical relevance concerning biomarkers should be based on, and respectful of the best scientific, legal and ethical evidence available. When, however, a conflict should arise, before any decision is taken a thorough risk-benefit analysis should be done, at the beginning of the process and after listening to the workers and the management involved, by the occupational physician or scientist, based on his/her professional experience, independent judgement and individual responsibility. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Oxygen flux as an indicator of physiological stress in aquatic organisms: a real-time biomonitoring system of water quality

NASA Astrophysics Data System (ADS)

Sanchez, Brian C.; Yale, Gowri; Chatni, Rameez; Ochoa-Acuña, Hugo G.; Porterfield, D. Marshall; Mclamore, Eric S.; Sepúlveda, María S.

2009-05-01

The detection of harmful chemicals and biological agents in real time is a critical need for protecting water quality. We studied the real-time effects of five environmental contaminants with differing modes of action (atrazine, pentachlorophenol, cadmium chloride, malathion, and potassium cyanide) on respiratory oxygen consumption in 2-day post-fertilization fathead minnow (Pimephales promelas) eggs. Our objective was to assess the sensitivity of fathead minnow eggs using the self-referencing micro-optrode technique to detect instantaneous changes in oxygen consumption after brief exposures to low concentrations of contaminants. Oxygen consumption data indicated that the technique is indeed sensitive enough to reliably detect physiological alterations induced by all contaminants. After 2 h of exposure, we identified significant increases in oxygen consumption upon exposure to pentachlorophenol (100 and 1000 μg/L), cadmium chloride (0.0002 and 0.002 μg/L), and atrazine (150 μg/L). In contrast, we observed a significant decrease in oxygen flux after exposures to potassium cyanide (5.2, 22, and 44 μg/L) and atrazine (1500 μg/L). No effects were detected after exposures to malathion (200 and 340 μg/L). We have also tested the sensitivity of Daphnia magna embryos as another animal model for real-time environmental biomonitoring. Our results are so far encouraging and support further development of this technology as a physiologically coupled biomonitoring tool for the detection of environmental toxicants.

Can we use modelling methodologies to assess airborne benzo[a]pyrene from biomonitors? A comprehensive evaluation approach

NASA Astrophysics Data System (ADS)

Ratola, N.; Jiménez-Guerrero, P.

2015-09-01

Biomonitoring data available on levels of atmospheric polycyclic aromatic hydrocarbons (PAHs) in pine needles from the Iberian Peninsula was used to estimate air concentrations of benzo[a]pyrene (BaP) and, at the same time, fuelled the comparison with chemistry transport model representations. Simulations with the modelling system WRF + CHIMERE were validated against data from the European Monitoring and Evaluation Programme (EMEP) air sampling network and using modelled atmospheric concentrations as a consistent reference in order to compare the performance of vegetation-to-air estimating methods. A spatial and temporal resolution of 9 km and 1 h was implemented. The field-based database relied on a pine needles sampling scheme comprising 33 sites in Portugal and 37 sites in Spain complemented with the BaP measurements available from the EMEP sites. The ability of pine needles to act as biomonitoring markers for the atmospheric concentrations of BaP was estimated converting the levels obtained in pine needles into air concentrations by six different approaches, one of them presenting realistic concentrations when compared to the modelled atmospheric values. The justification for this study is the gaps still existing in the knowledge of the life cycles of semi-volatile organic compounds (SVOCs), particularly the partition processes between air and vegetation. The strategy followed in this work allows the definition of the transport patterns (e.g. dispersion) established by the model for atmospheric concentrations and the estimated values in vegetation.

Conceptual framework for a Danish human biomonitoring program

PubMed Central

Thomsen, Marianne; Knudsen, Lisbeth E; Vorkamp, Katrin; Frederiksen, Marie; Bach, Hanne; Bonefeld-Jorgensen, Eva Cecilie; Rastogi, Suresch; Fauser, Patrik; Krongaard, Teddy; Sorensen, Peter Borgen

2008-01-01

The aim of this paper is to present the conceptual framework for a Danish human biomonitoring (HBM) program. The EU and national science-policy interface, that is fundamental for a realization of the national and European environment and human health strategies, is discussed, including the need for a structured and integrated environmental and human health surveillance program at national level. In Denmark, the initiative to implement such activities has been taken. The proposed framework of the Danish monitoring program constitutes four scientific expert groups, i.e. i. Prioritization of the strategy for the monitoring program, ii. Collection of human samples, iii. Analysis and data management and iv. Dissemination of results produced within the program. This paper presents the overall framework for data requirements and information flow in the integrated environment and health surveillance program. The added value of an HBM program, and in this respect the objectives of national and European HBM programs supporting environmental health integrated policy-decisions and human health targeted policies, are discussed. In Denmark environmental monitoring has been prioritized by extensive surveillance systems of pollution in oceans, lakes and soil as well as ground and drinking water. Human biomonitoring has only taken place in research programs and few incidences of e.g. lead contamination. However an arctic program for HBM has been in force for decades and from the preparations of the EU-pilot project on HBM increasing political interest in a Danish program has developed. PMID:18541069

A pilot study on the feasibility of European harmonized human biomonitoring: Strategies towards a common approach, challenges and opportunities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Casteleyn, L., E-mail: Ludwine.Casteleyn@med.kuleuven.be; Dumez, B.; Becker, K.

In 2004 the European Commission and Member States initiated activities towards a harmonized approach for Human Biomonitoring surveys throughout Europe. The main objective was to sustain environmental health policy by building a coherent and sustainable framework and by increasing the comparability of data across countries. A pilot study to test common guidelines for setting up surveys was considered a key step in this process. Through a bottom-up approach that included all stakeholders, a joint study protocol was elaborated. From September 2011 till February 2012, 17 European countries collected data from 1844 mother–child pairs in the frame of DEMOnstration of amore » study to COordinate and Perform Human Biomonitoring on a European Scale (DEMOCOPHES). Mercury in hair and urinary cadmium and cotinine were selected as biomarkers of exposure covered by sufficient analytical experience. Phthalate metabolites and Bisphenol A in urine were added to take into account increasing public and political awareness for emerging types of contaminants and to test less advanced markers/markers covered by less analytical experience. Extensive efforts towards chemo-analytical comparability were included. The pilot study showed that common approaches can be found in a context of considerable differences with respect to experience and expertize, socio-cultural background, economic situation and national priorities. It also evidenced that comparable Human Biomonitoring results can be obtained in such context. A European network was built, exchanging information, expertize and experiences, and providing training on all aspects of a survey. A key challenge was finding the right balance between a rigid structure allowing maximal comparability and a flexible approach increasing feasibility and capacity building. Next steps in European harmonization in Human Biomonitoring surveys include the establishment of a joint process for prioritization of substances to cover and biomarkers to develop, linking biomonitoring surveys with health examination surveys and with research, and coping with the diverse implementations of EU regulations and international guidelines with respect to ethics and privacy. - Highlights: • A common European Human Biomonitoring (HBM) survey protocol was developed through a bottom-up approach. • A joint process for prioritization was established to select a limited set of biomarkers, some covered by experience and others for emerging substances. • The protocol was tested in a pilot study, resulting in HBM results comparable on a European scale which sustained environmental health policy. • Ethics and privacy regulations were not an obstacle for transnational harmonization.« less

Biomonitoring: Guide for the Use of Biological Endpoints in Monitoring Species, Habitats, and Projects

DTIC Science & Technology

2007-11-01

et al ., 1991; Welsh and Ollivier 1998), behavior (Daly et al ., 1995; Maltby et al ., 2002 ...include changes in species diversity and community structure (Karr 1981; Bramblett and Fausch 1991; Barbour et al ., 1999; Zweig and Rabeni 2001; Martin et ...programs in aquatic habitats; these programs typically employ benthic invertebrates such as molluscs (Maltby et al ., 2002 ; Applied Biomonitoring

A pilot study on the feasibility of European harmonized human biomonitoring: Strategies towards a common approach, challenges and opportunities.

PubMed

Casteleyn, L; Dumez, B; Becker, K; Kolossa-Gehring, M; Den Hond, E; Schoeters, G; Castaño, A; Koch, H M; Angerer, J; Esteban, M; Exley, K; Sepai, O; Bloemen, L; Horvat, M; Knudsen, L E; Joas, A; Joas, R; Biot, P; Koppen, G; Dewolf, M-C; Katsonouri, A; Hadjipanayis, A; Cerná, M; Krsková, A; Schwedler, G; Fiddicke, U; Nielsen, J K S; Jensen, J F; Rudnai, P; Közepésy, S; Mulcahy, M; Mannion, R; Gutleb, A C; Fischer, M E; Ligocka, D; Jakubowski, M; Reis, M F; Namorado, S; Lupsa, I-R; Gurzau, A E; Halzlova, K; Jajcaj, M; Mazej, D; Tratnik Snoj, J; Posada, M; López, E; Berglund, M; Larsson, K; Lehmann, A; Crettaz, P; Aerts, D

2015-08-01

In 2004 the European Commission and Member States initiated activities towards a harmonized approach for Human Biomonitoring surveys throughout Europe. The main objective was to sustain environmental health policy by building a coherent and sustainable framework and by increasing the comparability of data across countries. A pilot study to test common guidelines for setting up surveys was considered a key step in this process. Through a bottom-up approach that included all stakeholders, a joint study protocol was elaborated. From September 2011 till February 2012, 17 European countries collected data from 1844 mother-child pairs in the frame of DEMOnstration of a study to COordinate and Perform Human Biomonitoring on a European Scale (DEMOCOPHES).(1) Mercury in hair and urinary cadmium and cotinine were selected as biomarkers of exposure covered by sufficient analytical experience. Phthalate metabolites and Bisphenol A in urine were added to take into account increasing public and political awareness for emerging types of contaminants and to test less advanced markers/markers covered by less analytical experience. Extensive efforts towards chemo-analytical comparability were included. The pilot study showed that common approaches can be found in a context of considerable differences with respect to experience and expertize, socio-cultural background, economic situation and national priorities. It also evidenced that comparable Human Biomonitoring results can be obtained in such context. A European network was built, exchanging information, expertize and experiences, and providing training on all aspects of a survey. A key challenge was finding the right balance between a rigid structure allowing maximal comparability and a flexible approach increasing feasibility and capacity building. Next steps in European harmonization in Human Biomonitoring surveys include the establishment of a joint process for prioritization of substances to cover and biomarkers to develop, linking biomonitoring surveys with health examination surveys and with research, and coping with the diverse implementations of EU regulations and international guidelines with respect to ethics and privacy. Copyright © 2014 Elsevier Inc. All rights reserved.

Human biomonitoring data interpretation and ethics; obstacles or surmountable challenges?

PubMed Central

Sepai, Ovnair; Collier, Clare; Van Tongelen, Birgit; Casteleyn, Ludwine

2008-01-01

The use of human samples to assess environmental exposure and uptake of chemicals is more than an analytical exercise and requires consideration of the utility and interpretation of data as well as due consideration of ethical issues. These aspects are inextricably linked. In 2004 the EC expressed its commitment to the development of a harmonised approach to human biomonitoring (HBM) by including an action in the EU Environment and Health Strategy to develop a Human Biomonitoring Pilot Study. This further underlined the need for interpretation strategies as well as guidance on ethical issues. A workshop held in December 2006 brought together stakeholders from academia, policy makers as well as non-governmental organisations and chemical industry associations to a two day workshop built a mutual understanding of the issues in an open and frank discussion forum. This paper describes the discussion and recommendations from the workshop. The workshop developed key recommendations for a Pan-European HBM Study: 1. A strategy for the interpretation of human biomonitoring data should be developed. 2. The pilot study should include the development of a strategy to integrate health data and environmental monitoring with human biomonitoring data at national and international levels. 3. Communication strategies should be developed when designing the study and evolve as the study continues. 4. Early communication with stakeholders is essential to achieve maximum efficacy of policy developments and facilitate subsequent monitoring. 5. Member states will have to apply individually for project approval from their National Research Ethics Committees. 6. The study population needs to have sufficient information on the way data will be gathered, interpreted and disseminated and how samples will be stored and used in the future (if biobanking) before they can give informed consent. 7. The participants must be given the option of anonymity. This has an impact on follow-up. 8. The pilot study should develop guidelines and best practice for Ethics for pan European studies. In conclusion all participants felt there that there has to be stakeholder involvement in any planned pan-European Human Biomonitoring Study and the format of the workshop was appropriate for such dialogue. PMID:18541067

Risk communication and human biomonitoring: which practical lessons from the Belgian experience are of use for the EU perspective?

PubMed Central

Keune, Hans; Morrens, Bert; Loots, Ilse

2008-01-01

Background In order to investigate and monitor environmental health in Flanders (the Dutch speaking part of Belgium), the Flemish government funded the Centre of Expertise for Environment and Health, which started a human biomonitoring campaign in 2001. In addition to environmental health experts measuring environmental pollutants and health effects in human beings, social scientific experts at the Centre focus on risk communication associated with the human biomonitoring campaign. Methods In the literature about risk communication an evolution can be traced from traditional, one-way communication, restricted to the dissemination of information from experts to the public, to more modern, two-way risk communication, with a focus on participation and cooperation between scientists, policy-makers and the public. Within the Centre of Expertise for Environment and Health this discourse was first translated into some general principles and guidelines for external communication, at a 'Ten Commandments level'. These principles needed to be incorporated in the day-to-day practice of human biomonitoring research. Results The social scientific experts at the Centre developed a combined risk communication strategy. On the one hand the strategy consists of traditional risk communication for external communication purposes, for example information meetings and digital newsletters. On the other hand it consists of a step by step approach of incorporating more modern risk communication, for example a risk perception questionnaire, dialogical experiments for involving local stakeholders, and an action-plan for interpreting results for policy making. Conclusion With a parallel strategy of traditional and modern communication, of external and internal reflection, and through different social scientific projects, the Flemish Centre of Expertise of Environment and Health incorporates risk communication in the day-to-day practice of human biomonitoring research. A direct and continuous involvement of the social scientist, an openness between all colleagues involved, and the awareness of a fine balance between quality and practicability are important success factors. These lessons may be helpful and inspirational for a European human biomonitoring project. PMID:18541065

[Validation of two indices of biological integrity (IBI) for the Angulo River subbasin in Central Mexico].

PubMed

Ramírez-Herrejón, Juan Pablo; Mercado-Silva, Norman; Medina-Nava, Martina; Domínguez-Domínguez, Omar

2012-12-01

Efforts to halt freshwater ecosystem degradation in central Mexico can benefit from using bio-monitoring tools that reflect the condition of their biotic integrity. We analyzed the applicability of two fish-based indices of biotic integrity using data from lotic and lentic systems in the Angulo River subbasin (Lerma-Chapala basin). Both independent data from our own collections during two consecutive years, and existing information detailing the ecological attributes of each species, were used to calculate indices of biological integrity for 16 sites in lotic and lentic habitats. We assessed environmental quality by combining independent evaluations water and habitat quality for each site. We found sites with poor, regular and good biotic integrity. Our study did not find sites with good environmental quality. Fish-based IBI scores were strongly and significantly correlated with scores from independent environmental assessment techniques. IBI scores were adequate at representing environmental conditions in most study sites. These results expand the area where a lotic system fish-based IBI can be used, and constitute an initial validation of a lentic system fish-based IBI. Our results suggest that these bio-monitoring tools can be used in future conservation efforts in freshwater ecosystems in the Middle Lerma Basin.

Development of robust behaviour recognition for an at-home biomonitoring robot with assistance of subject localization and enhanced visual tracking.

PubMed

Imamoglu, Nevrez; Dorronzoro, Enrique; Wei, Zhixuan; Shi, Huangjun; Sekine, Masashi; González, José; Gu, Dongyun; Chen, Weidong; Yu, Wenwei

2014-01-01

Our research is focused on the development of an at-home health care biomonitoring mobile robot for the people in demand. Main task of the robot is to detect and track a designated subject while recognizing his/her activity for analysis and to provide warning in an emergency. In order to push forward the system towards its real application, in this study, we tested the robustness of the robot system with several major environment changes, control parameter changes, and subject variation. First, an improved color tracker was analyzed to find out the limitations and constraints of the robot visual tracking considering the suitable illumination values and tracking distance intervals. Then, regarding subject safety and continuous robot based subject tracking, various control parameters were tested on different layouts in a room. Finally, the main objective of the system is to find out walking activities for different patterns for further analysis. Therefore, we proposed a fast, simple, and person specific new activity recognition model by making full use of localization information, which is robust to partial occlusion. The proposed activity recognition algorithm was tested on different walking patterns with different subjects, and the results showed high recognition accuracy.

Development of Robust Behaviour Recognition for an at-Home Biomonitoring Robot with Assistance of Subject Localization and Enhanced Visual Tracking

PubMed Central

Imamoglu, Nevrez; Dorronzoro, Enrique; Wei, Zhixuan; Shi, Huangjun; González, José; Gu, Dongyun; Yu, Wenwei

2014-01-01

Our research is focused on the development of an at-home health care biomonitoring mobile robot for the people in demand. Main task of the robot is to detect and track a designated subject while recognizing his/her activity for analysis and to provide warning in an emergency. In order to push forward the system towards its real application, in this study, we tested the robustness of the robot system with several major environment changes, control parameter changes, and subject variation. First, an improved color tracker was analyzed to find out the limitations and constraints of the robot visual tracking considering the suitable illumination values and tracking distance intervals. Then, regarding subject safety and continuous robot based subject tracking, various control parameters were tested on different layouts in a room. Finally, the main objective of the system is to find out walking activities for different patterns for further analysis. Therefore, we proposed a fast, simple, and person specific new activity recognition model by making full use of localization information, which is robust to partial occlusion. The proposed activity recognition algorithm was tested on different walking patterns with different subjects, and the results showed high recognition accuracy. PMID:25587560

Lessons learnt on recruitment and fieldwork from a pilot European human biomonitoring survey.

PubMed

Fiddicke, Ulrike; Becker, Kerstin; Schwedler, Gerda; Seiwert, Margarete; Joas, Reinhard; Joas, Anke; Biot, Pierre; Aerts, Dominique; Casteleyn, Ludwine; Dumez, Birgit; Castaño, Argelia; Esteban, Marta; Angerer, Jürgen; Koch, Holger M; Schoeters, Greet; Den Hond, Elly; Sepai, Ovnair; Exley, Karen; Knudsen, Lisbeth E; Horvat, Milena; Bloemen, Louis; Katsonouri, Andromachi; Hadjipanayis, Adamos; Cerna, Milena; Krsková, Andrea; Jensen, Janne Fangel; Nielsen, Jeanette K S; Rudnai, Peter; Közepésy, Szilvia; Gutleb, Arno C; Fischer, Marc E; Ligocka, Danuta; Kamińska, Joanna; Reis, M Fátima; Namorado, Sónia; Lupsa, Ioana-Rodica; Gurzau, Anca E; Halzlová, Katarína; Mazej, Darja; Tratnik, Janja Snoj; Rivas, Teresa C; Gómez, Silvia; Berglund, Marika; Larsson, Kristin; Lehmann, Andrea; Crettaz, Pierre; Dewolf, Marie-Christine; Burns, Damien; Kellegher, Anne; Kolossa-Gehring, Marike

2015-08-01

Within the European Environment and Health Action Plan an initiative to establish a coherent human biomonitoring approach in Europe was started. The project COPHES (COnsortium to Perform Human biomonitoring on a European Scale ) developed recommendations for a harmonized conduct of a human biomonitoring (HBM) survey which came into action as the pilot study DEMOCOPHES (DEMOnstration of a study to COordinate and Perform Human biomonitoring on a European Scale). Seventeen European countries conducted a survey with harmonized instruments for, inter alia, recruitment, fieldwork and sampling, in autumn/winter 2011/2012. Based on the countries' experiences of conducting the pilot study, following lessons learnt were compiled: the harmonized fieldwork instruments (basic questionnaire, urine and hair sampling) turned out to be very valuable for future HBM surveys on the European scale. A school approach was favoured by most of the countries to recruit school-aged children according to the established guidelines and country specific experiences. To avoid a low participation rate, intensive communication with the involved institutions and possible participants proved to be necessary. The communication material should also include information on exclusion criteria and offered incentives. Telephone contact to the participants the day before fieldwork during the survey can prevent the forgetting of appointments and first morning urine samples. To achieve comparable results on the European scale, training of interviewers in all issues of recruitment, fieldwork and sampling through information material and training sessions is crucial. A survey involving many European countries needs time for preparation and conduct. Materials for quality control prepared for all steps of recruitment, fieldwork and sampling proved to be important to warrant reliable results. Copyright © 2014 Elsevier Inc. All rights reserved.

Development of biomonitoring equivalents for barium in urine and plasma for interpreting human biomonitoring data.

PubMed

Poddalgoda, Devika; Macey, Kristin; Assad, Henry; Krishnan, Kannan

2017-06-01

The objectives of the present work were: (1) to assemble population-level biomonitoring data to identify the concentrations of urinary and plasma barium across the general population; and (2) to derive biomonitoring equivalents (BEs) for barium in urine and plasma in order to facilitate the interpretation of barium concentrations in the biological matrices. In population level biomonitoring studies, barium has been measured in urine in the U.S. (NHANES study), but no such data on plasma barium levels were identified. The BE values for plasma and urine were derived from U.S. EPA's reference dose (RfD) of 0.2 mg/kg bw/d, based on a lower confidence limit on the benchmark dose (BMDL 05 ) of 63 mg/kg bw/d. The plasma BE (9 μg Ba/L) was derived by regression analysis of the near-steady-state plasma concentrations associated with the administered doses in animals exposed to barium chloride dihydrate in drinking water for 2-years in a NTP study. Using a human urinary excretion fraction of 0.023, a BE for urinary barium (0.19 mg/L or 0.25 mg/g creatinine) was derived for US EPA's RfD. The median and the 95 th percentile barium urine concentrations of the general population in U.S. are below the BE determined in this study, indicating that the population exposure to inorganic barium is expected to be below the exposure guidance value of 0.2 mg/kg bw/d. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

Urban health and ecology: the promise of an avian biomonitoring tool

PubMed Central

Pollack, Lea; Ondrasek, Naomi R.

2017-01-01

Abstract Urban-dwelling birds have the potential to serve as powerful biomonitors that reveal the impact of environmental change due to urbanization. Specifically, urban bird populations can be used to survey cities for factors that may pose both public and wildlife health concerns. Here, we review evidence supporting the use of avian biomonitors to identify threats associated with urbanization, including bioaccumulation of toxicants and the dysregulation of behavior and physiology by related stressors. In addition, we consider the use of birds to examine how factors in the urban environment can impact immunity against communicable pathogens. By studying the behavior, physiology, and ecology of urban bird populations, we can elucidate not only how avian populations are responding to environmental change, but also how unintended consequences of urbanization affect the well-being of human and non-human inhabitants. PMID:29491978

Urban health and ecology: the promise of an avian biomonitoring tool.

PubMed

Pollack, Lea; Ondrasek, Naomi R; Calisi, Rebecca

2017-04-01

Urban-dwelling birds have the potential to serve as powerful biomonitors that reveal the impact of environmental change due to urbanization. Specifically, urban bird populations can be used to survey cities for factors that may pose both public and wildlife health concerns. Here, we review evidence supporting the use of avian biomonitors to identify threats associated with urbanization, including bioaccumulation of toxicants and the dysregulation of behavior and physiology by related stressors. In addition, we consider the use of birds to examine how factors in the urban environment can impact immunity against communicable pathogens. By studying the behavior, physiology, and ecology of urban bird populations, we can elucidate not only how avian populations are responding to environmental change, but also how unintended consequences of urbanization affect the well-being of human and non-human inhabitants.

A failure management prototype: DR/Rx

NASA Technical Reports Server (NTRS)

Hammen, David G.; Baker, Carolyn G.; Kelly, Christine M.; Marsh, Christopher A.

1991-01-01

This failure management prototype performs failure diagnosis and recovery management of hierarchical, distributed systems. The prototype, which evolved from a series of previous prototypes following a spiral model for development, focuses on two functions: (1) the diagnostic reasoner (DR) performs integrated failure diagnosis in distributed systems; and (2) the recovery expert (Rx) develops plans to recover from the failure. Issues related to expert system prototype design and the previous history of this prototype are discussed. The architecture of the current prototype is described in terms of the knowledge representation and functionality of its components.

MACROINVERTEBRATE ASSEMBLAGES OF THE ST. CROIX AND WISCONSIN RIVERS: AN INITIAL APPLICATION OF THE LARGE RIVER BIOASSESSMENT PROTOCOL (LR-BP) IN THE MIDWESTERN UNITED STATES

EPA Science Inventory

Since passage of the Clean Water Act, government agencies have made extensive use of biomonitoring protocols to report on the quality of wadeable streams and rivers. Non-wadeable systems have been largely overlooked because of sampling difficulties and a lack of appropriate metho...

Evaluation of a Home Biomonitoring Autonomous Mobile Robot.

PubMed

Dorronzoro Zubiete, Enrique; Nakahata, Keigo; Imamoglu, Nevrez; Sekine, Masashi; Sun, Guanghao; Gomez, Isabel; Yu, Wenwei

2016-01-01

Increasing population age demands more services in healthcare domain. It has been shown that mobile robots could be a potential solution to home biomonitoring for the elderly. Through our previous studies, a mobile robot system that is able to track a subject and identify his daily living activities has been developed. However, the system has not been tested in any home living scenarios. In this study we did a series of experiments to investigate the accuracy of activity recognition of the mobile robot in a home living scenario. The daily activities tested in the evaluation experiment include watching TV and sleeping. A dataset recorded by a distributed distance-measuring sensor network was used as a reference to the activity recognition results. It was shown that the accuracy is not consistent for all the activities; that is, mobile robot could achieve a high success rate in some activities but a poor success rate in others. It was found that the observation position of the mobile robot and subject surroundings have high impact on the accuracy of the activity recognition, due to the variability of the home living daily activities and their transitional process. The possibility of improvement of recognition accuracy has been shown too.

The role of toxicology to characterize biomarkers for agrochemicals with potential endocrine activities.

PubMed

Mantovani, Alberto; Maranghi, Francesca; La Rocca, Cinzia; Tiboni, Gian Mario; Clementi, Maurizio

2008-09-01

The paper discusses current knowledge and possible research priorities on biomarkers of exposure, effect and susceptibility for potential endocrine activities of agrochemicals (dicarboximides, ethylene bisdithiocarbammates, triazoles, etc.). Possible widespread, multiple-pathway exposure to agrochemicals highlights the need to assess internal exposure of animals or humans, which is the most relevant exposure measure for hazard and risk estimation; however, exposure data should be integrated by early indicators predictive of possible health effects, particularly for vulnerable groups such as mother-child pairs. Research need include: non-invasive biomarkers for children biomonitoring; novel biomarkers of total exposure to measure whole endocrine disrupter-related burden; characterization of biomarkers of susceptibility, including the role of markers of nutritional status; anchoring early molecular markers to established toxicological endpoints to support their predictivity; integrating "omics"-based approaches in a system-toxicology framework. As biomonitoring becomes increasingly important in the environment-and-health scenario, toxicologists can substantially contribute both to the characterization of new biomarkers and to the predictivity assessment and improvement of the existing ones.

Differentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR.

PubMed

Feng, Lifang; Miao, Wei; Wu, Yuxuan

2007-02-15

Tributyltin (TBT) is widely used as antifouling paints, agriculture biocides, and plastic stabilizers around the world, resulting in great pollution problem in aquatic environments. However, it has been short of the biomonitor to detect TBT in freshwater. We constructed the suppression subtractive hybridization library of Tetrahymena thermophila exposed to TBT, and screened out 101 Expressed Sequence Tags whose expressions were significantly up- or down-regulated with TBT treatment. From this, a series of genes related to the TBT toxicity were discovered, such as glutathione-S-transferase gene (down-regulated), plasma membrane Ca2+ ATPase isoforms 3 gene (up-regulated) and NgoA (up-regulated). Furthermore, their expressions under different concentrations of TBT treatment (0.5-40 ppb) were detected by real time fluorescent quantitative PCR. The differentially expressed genes of T. thermophila in response to TBT were identified, which provide the basic to make Tetrahymena as a sensitive, rapid and convenient TBT biomonitor in freshwater based on rDNA inducible expression system.

The comet assay as a tool for human biomonitoring studies: the ComNet project.

PubMed

Collins, Andrew; Koppen, Gudrun; Valdiglesias, Vanessa; Dusinska, Maria; Kruszewski, Marcin; Møller, Peter; Rojas, Emilio; Dhawan, Alok; Benzie, Iris; Coskun, Erdem; Moretti, Massimo; Speit, Günter; Bonassi, Stefano

2014-01-01

The comet assay is widely used in human biomonitoring to measure DNA damage as a marker of exposure to genotoxic agents or to investigate genoprotective effects. Studies often involve small numbers of subjects, and design may be sub-optimal in other respects. In addition, comet assay protocols in use in different laboratories vary significantly. In spite of these difficulties, it is appropriate to carry out a pooled analysis of all available comet assay biomonitoring data, in order to establish baseline parameters of DNA damage, and to investigate associations between comet assay measurements and factors such as sex, age, smoking status, nutrition, lifestyle, etc. With this as its major objective, the ComNet project has recruited almost 100 research groups willing to share datasets. Here we provide a background to this project, discussing the history of the comet assay and practical issues that can critically affect its performance. We survey its diverse applications in biomonitoring studies, including environmental and occupational exposure to genotoxic agents, genoprotection by dietary and other factors, DNA damage associated with various diseases, and intrinsic factors that affect DNA damage levels in humans. We examine in depth the quality of data from a random selection of studies, from an epidemiological and statistical point of view. Copyright © 2013 Elsevier B.V. All rights reserved.

A new scheme for biomonitoring heavy metal concentrations in semi-natural wetlands.

PubMed

Batzias, A F; Siontorou, C G

2008-10-30

This work introduces a semi-natural wetland biomonitoring framework for heavy metal concentrations based on a robust dynamic integration between biological assemblages and relevant biosensors. The cooperative/synergistic scheme developed minimizes uncertainty and monitoring costs and increases reliability of pollution control and abatement. Attention is given to establishing a fully functioning and reliable network approach for monitoring inflows and achieving dose-response relations and calibration of biomonitoring species. The biomonitoring network initially consists of both, biosensors and species, as a validation phase in each wetland of the surveillance area; once the species monitoring efficiency is verified by the biosensors, the biosensor network moves to the next wetland and so on, following a circular pattern until all area wetlands have a fully functional natural monitoring scheme. By means of species recalibration with periodic revisiting of the biosensors, the scheme progressively reaches a quasi steady-state (including seasonality), thus ensuring reliability and robustness. This framework, currently pilot-tested in Voiotia, Greece, for assessing chromium levels, has been built to cover short-, medium- and long-term monitoring requirements. The results gathered so far, support the employment of the proposed scheme in heavy metal monitoring, and, further, arise the need for volunteer involvement to achieve long-term viability.

Can nail, hair and urine be used for biomonitoring of human exposure to perfluorooctane sulfonate and perfluorooctanoic acid?

PubMed

Li, Jingguang; Guo, Feifei; Wang, Yuxin; Zhang, Jialing; Zhong, Yuxin; Zhao, Yunfeng; Wu, Yongning

2013-03-01

Because of the disadvantages of invasive sampling, it is desirable to explore non-invasive matrices for human biomonitoring of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA). The aim of this study was to evaluate the application of nail, hair and urine for human biomonitoring of PFOS and PFOA. The concentrations of PFOS and PFOA in matched nail, hair, urine and serum samples collected from 64 donors were measured. The chemicals of interest were detected with high detection frequency in these matrices (90%-100%) except for PFOA in urine samples (56%). Generally, the gender influences on the levels of PFOS and PFOA in these non-invasive matrices were in agreement with that in serum. For PFOS, the coefficients of Spearman correlation between serum samples and nail, hair and urine samples were 0.786 (p<0.001), 0.545 (p<0.001) and 0.302 (p<0.05), respectively. For PFOA, the correlation was only observed between nail samples and serum samples with a correlation coefficient of 0.299 (p<0.05). The results suggested that nail has more potential than hair and urine to be applied in human biomonitoring for PFOS and PFOA in general populations. Copyright © 2013 Elsevier Ltd. All rights reserved.

“In vitro” Implantation Technique Based on 3D Printed Prosthetic Prototypes

NASA Astrophysics Data System (ADS)

Tarnita, D.; Boborelu, C.; Geonea, I.; Malciu, R.; Grigorie, L.; Tarnita, D. N.

2018-06-01

In this paper, Rapid Prototyping ZCorp 310 system, based on high-performance composite powder and on resin-high strength infiltration system and three-dimensional printing as a manufacturing method are used to obtain physical prototypes of orthopaedic implants and prototypes of complex functional prosthetic systems directly from the 3D CAD data. These prototypes are useful for in vitro experimental tests and measurements to optimize and obtain final physical prototypes. Using a new elbow prosthesis model prototype obtained by 3D printing, the surgical technique of implantation is established. Surgical implantation was performed on male corpse elbow joint.

End effector monitoring system: An illustrated case of operational prototyping

NASA Technical Reports Server (NTRS)

Malin, Jane T.; Land, Sherry A.; Thronesbery, Carroll

1994-01-01

Operational prototyping is introduced to help developers apply software innovations to real-world problems, to help users articulate requirements, and to help develop more usable software. Operational prototyping has been applied to an expert system development project. The expert system supports fault detection and management during grappling operations of the Space Shuttle payload bay arm. The dynamic exchanges among operational prototyping team members are illustrated in a specific prototyping session. We discuss the requirements for operational prototyping technology, types of projects for which operational prototyping is best suited and when it should be applied to those projects.

Cell-Phone Tower Power System Prototype Testing for Verizon Wireless |

Science.gov Websites

Verizon Wireless Cell-Phone Tower Power System Prototype Testing for Verizon Wireless For Verizon Wireless Advanced Manufacturing Research | NREL Cell-Phone Tower Power System Prototype Testing for , NREL tested a new cell-phone tower power system prototype based on DC interconnection and photovoltaics

Human biomonitoring: Science and policy for a healthy future, April 17-19, 2016, Berlin, Germany.

PubMed

Joas, Anke; Schwedler, Gerda; Choi, Judy; Kolossa-Gehring, Marike

2017-03-01

Following the success of the 1st International Conference on Human Biomonitoring (HBM) in Berlin in 2010, the 2nd International Conference on Human Biomonitoring took place in Berlin from April 17-19, 2016 for an exchange and updates among participants on all aspects relating to HBM. Entitled "Science and Policy for a Healthy Future", the conference brought together international experts from the scientific sector, politics, authorities, industry, non-governmental organizations (NGOs), and other involved associations. The conference took a critical look at today's chemicals that have a potential impact on human health and should be investigated as a matter of priority. It also discussed current activities and research efforts on HBM occurring worldwide, presented HBM success stories, and emphasized areas, where further research and focus are needed to improve the use of HBM for policy making. In many countries, HBM has been proven to be a useful tool and warning system to indicate problematic human exposure to pollutants and to evaluate the effectiveness of existing chemicals policy and regulations. However, important challenges remain such as exposure assessment of mixtures of chemicals, the development of analytical methods to detect new chemicals of concern (e.g., substitutes for phthalates), the identification of exposure sources, and the assessment of the impact of exposure on health. This brief report summarizes the discussions and contributions from this conference, which was jointly organized by the German Federal Environment Agency (UBA) and the German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (BMUB). Copyright © 2017.

Inkjet-/3D-/4D-printed autonomous wearable RF modules for biomonitoring, positioning and sensing applications

NASA Astrophysics Data System (ADS)

Bito, Jo; Bahr, Ryan; Hester, Jimmy; Kimionis, John; Nauroze, Abdullah; Su, Wenjing; Tehrani, Bijan; Tentzeris, Manos M.

2017-05-01

In this paper, numerous inkjet-/3D-/4D-printed wearable flexible antennas, RF electronics, modules and sensors fabricated on paper and other polymer (e.g. LCP) substrates are introduced as a system-level solution for ultra-low-cost mass production of autonomous Biomonitoring, Positioning and Sensing applications. This paper briefly discusses the state-of-the-art area of fully-integrated wearable wireless sensor modules on paper or flexible LCP and show the first ever 4D sensor module integration on paper, as well as numerous 3D and 4D multilayer paper-based and LCP-based RF/microwave, flexible and wearable structures, that could potentially set the foundation for the truly convergent wireless sensor ad-hoc "on-body networks of the future with enhanced cognitive intelligence and "rugged" packaging. Also, some challenges concerning the power sources of "nearperpetual" wearable RF modules, including flexible miniaturized batteries as well as power-scavenging approaches involving electromagnetic and solar energy forms are discuessed. The final step of the paper will involve examples from mmW wearable (e.g. biomonitoring) antennas and RF modules, as well as the first examples of the integration of inkjet-printed nanotechnology-based (e.g.CNT) sensors on paper and organic substrates for Internet of Things (IoT) applications. It has to be noted that the paper will review and present challenges for inkjetprinted organic active and nonlinear devices as well as future directions in the area of environmentally-friendly "green") wearable RF electronics and "smart-skin conformal sensors.

Heavy metals phytoremediation potential of Hevea brasiliensis in Bentong, Malaysia

NASA Astrophysics Data System (ADS)

Yusof, Muhammad Jefri Mohd; Latif, Mohd Talip; Yusoff, Siti Fairus Mohd

2018-04-01

Biomonitoring uses living organisms to assess environmental quality and being preferred over conventional methods that use fully or semi-automatic gauges for its lower cost and practicality. Recently, higher plants are widely used for biomonitoring purposes by means of their species identification simplicity, larger availability of biological substantial, and easy to sample. In this study, samples of Hevea brasiliensis (i.e leaves, barks, and latex as well as surrounding soils) from outskirts of Pelangai, Bentong were tested for heavy metals by using inductively coupled plasma optical emission spectroscopy (ICP-OES). Enrichment factor of soils indicated that some metals (B, Ca, Cu, Mn, Pb, Zn, As and Na) were anthropogenic which most likely originated from traffic emissions. In addition, leaves trapped the most heavy metals compared to barks and latex. The accumulation of pollutants in those samples has identified biomonitoring abilities of Hevea brasiliensis.

Air Pollution Study in the Republic of Moldova Using Moss Biomonitoring Technique.

PubMed

Zinicovscaia, Inga; Hramco, Constantin; Duliu, Octavian G; Vergel, Konstantin; Culicov, Otilia A; Frontasyeva, Marina V; Duca, Gheorghe

2017-02-01

Moss biomonitoring using the species Hypnum cupressiforme (Hedw.) and Pleurocarpous sp was applied to study air pollution in the Republic of Moldova. A total of 41 elements (Na, Mg, Al, Cl, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Br, Rb, Sr, Zr, Cd, Sb, Cs, Ba, La, Ce, Nd, Sm, Eu, Gd, Tb, Tm, Yb, Hf, Ta, W, Pb, Th, and U) were determined by instrumental epithermal neutron activation analysis and atomic absorption spectrometry. Principal component analysis was used to identify and characterize different pollution sources. Geographical distribution maps were prepared to point out the regions most affected by air pollution and relate this to potential sources of contamination. Median values of the elements studied were compared with data from the European moss biomonitoring program. The cities of Chisinau and Balti were determined to experience particular environmental stress.

Biological Matrix Effects in Quantitative Tandem Mass Spectrometry-Based Analytical Methods: Advancing Biomonitoring

PubMed Central

Panuwet, Parinya; Hunter, Ronald E.; D’Souza, Priya E.; Chen, Xianyu; Radford, Samantha A.; Cohen, Jordan R.; Marder, M. Elizabeth; Kartavenka, Kostya; Ryan, P. Barry; Barr, Dana Boyd

2015-01-01

The ability to quantify levels of target analytes in biological samples accurately and precisely, in biomonitoring, involves the use of highly sensitive and selective instrumentation such as tandem mass spectrometers and a thorough understanding of highly variable matrix effects. Typically, matrix effects are caused by co-eluting matrix components that alter the ionization of target analytes as well as the chromatographic response of target analytes, leading to reduced or increased sensitivity of the analysis. Thus, before the desired accuracy and precision standards of laboratory data are achieved, these effects must be characterized and controlled. Here we present our review and observations of matrix effects encountered during the validation and implementation of tandem mass spectrometry-based analytical methods. We also provide systematic, comprehensive laboratory strategies needed to control challenges posed by matrix effects in order to ensure delivery of the most accurate data for biomonitoring studies assessing exposure to environmental toxicants. PMID:25562585

Prototyping with AIDA for a hospital pharmacy system.

PubMed

Molenaar, G C; Boon, W M

1987-01-01

The CENTRASYS system for the Hospital Pharmacy, developed as part of a research project of the Department of Medical Informatics is described. The role of AIDA, a fourth-generation software package, as a prototyping tool is discussed. It is concluded that AIDA facilitates prototyping and is also very suitable as a vehicle for systems in operation. It is further concluded that prototyping is of great help in the developmental phase of a project, but that great care has to be taken during evaluation of the prototypes: minimize the number of test sites and try to avoid that users become dependent on the system, because every prototype needs further tuning before it really becomes an operational system.

A PC based fault diagnosis expert system

NASA Technical Reports Server (NTRS)

Marsh, Christopher A.

1990-01-01

The Integrated Status Assessment (ISA) prototype expert system performs system level fault diagnosis using rules and models created by the user. The ISA evolved from concepts to a stand-alone demonstration prototype using OPS5 on a LISP Machine. The LISP based prototype was rewritten in C and the C Language Integrated Production System (CLIPS) to run on a Personal Computer (PC) and a graphics workstation. The ISA prototype has been used to demonstrate fault diagnosis functions of Space Station Freedom's Operation Management System (OMS). This paper describes the development of the ISA prototype from early concepts to the current PC/workstation version used today and describes future areas of development for the prototype.

Chemical contamination assessment in mangrove-lined Caribbean coastal systems using the oyster Crassostrea rhizophorae as biomonitor species.

PubMed

Aguirre-Rubí, Javier R; Luna-Acosta, Andrea; Etxebarría, Nestor; Soto, Manu; Espinoza, Félix; Ahrens, Michael J; Marigómez, Ionan

2018-05-01

This paper aims to contribute to the use of mangrove cupped oyster, Crassostrea rhizophorae, as a biomonitor species for chemical contamination assessment in mangrove-lined Caribbean coastal systems. Sampling was carried out in eight localities (three in Nicaragua and five in Colombia) with different types and levels of contamination. Oysters were collected during the rainy and dry seasons of 2012-2013 and the tissue concentrations of metals, polycyclic aromatic hydrocarbons (PAHs), and persistent organic pollutants (POPs) were determined. Low tissue concentrations of metals (except Hg) and PAHs; moderate-to-high tissue concentrations of Hg, hexachlorocyclohexanes (HCHs), and dichlorodiphenyl-trichloroethanes (DDTs); detectable levels of chlorpyrifos, polychlorinated biphenyls (PCBs) (mainly CB28, CB118, CB138 and CB 153) and brominated diphenyl ethers 85 (BDE85); and negligible levels of musks were recorded in Nicaraguan oysters. A distinct profile of POPs was identified in Colombia, where the tissue concentrations of PCBs and synthetic musk fragrances were low to moderate, and Ag, As, Cd, Pb, and PAHs ranged from moderate to extremely high. Overall, the values recorded for HCHs, DDTs and PCBs in Nicaraguan mangrove cupped oysters greatly exceeded the reference values in tissues of C. rhizophorae from the Wider Caribbean Region, whereas only the levels of PCBs were occasionally surpassed in Colombia. Different contaminant profiles were distinguished between oysters from Nicaragua and Colombia in radar plots constructed using the main groups of contaminants (metals, PAHs, musks, PCBs, and organochlorine pesticides (OCPs)). Likewise, integrated pollution indices revealed differences in the levels of contaminants. Moreover, the profiles and levels in oyster tissues also varied with season. Thus, principal component analysis clearly discriminated Nicaraguan and Colombian localities and, especially in Colombia, seasonal trends in chemical contamination and differences amongst localities were evidenced. The geographical and environmental disparity of the studied scenarios may represent to a large extent the diversity of mangrove-lined Caribbean coastal systems and therefore the present results support the use of C. rhizophorae as suitable biomonitor species at Caribbean regional scale, where seasonal variability is a major factor controlling pollutant mobility and bioavailability.

Text-Based On-Line Conferencing: A Conceptual and Empirical Analysis Using a Minimal Prototype.

ERIC Educational Resources Information Center

McCarthy, John C.; And Others

1993-01-01

Analyzes requirements for text-based online conferencing through the use of a minimal prototype. Topics discussed include prototyping with a minimal system; text-based communication; the system as a message passer versus the system as a shared data structure; and three exercises that showed how users worked with the prototype. (Contains 61…

Biomonitoring and biomarkers of organophosphate pesticides exposure - state of the art.

PubMed

Kapka-Skrzypczak, Lucyna; Cyranka, Małgorzata; Skrzypczak, Maciej; Kruszewski, Marcin

2011-01-01

Human biomonitoring provides an efficient and cost-effective way to identify and quantify exposure to chemical substances, including those having deleterious eff ects on human organisms. Once the risk of hazardous exposure has been identified and the mechanism of toxic eff ects has been elucidated, an ultimate decision about how to reduce exposure can be made. A particularly high risk of exposure to hazardous chemicals is associated with the use of pesticides in agriculture, especially the use of organophosphorous pesticides (OP), which are the most widely and commonly used insecticides worldwide. There is some strong evidence that chronic exposure to these compounds may have adverse eff ects on health. Exposure to pesticides has been associated with an increase in the incidence of non-Hodgkin's lymphoma, multiple myeloma, soft tissue sarcoma, lung sarcoma, and cancer of the pancreas, stomach, liver, bladder and gall bladder, Parkinson disease, Alzheimer disease, and reproductive outcomes. In view of these findings, the detection of populations at risk constitutes a very important topic. The biomonitoring studies on individuals exposed to pesticides have shown an elevated level of indicators of DNA damage, such as chromosomal aberrations (CA), sister chromatid exchanges (SCE), micronuclei (MN), and recently, single cell gel electrophoresis (SCGE). The cytogenetic markers of DNA damage have become very popular and useful in providing an analytical data for risk assessment, such as internal exposure doses and early biological eff ects of both occupational and environmental exposure to pesticides. The article describes the usefulness and the limitations of these biomarkers in biomonitoring studies of populations exposed to pesticides, with regard to the main routes of uptake and different matrices, which can be used to monitor risk assessment in occupational settings. The article also summarizes the latest reports about biomarkers of susceptibility, and mentions other biomarkers widely used in biomonitoring studies, such as pesticide or its metabolites level.

Biomonitors of atmospheric nitrogen deposition: potential uses and limitations.

PubMed

Díaz-Álvarez, Edison A; Lindig-Cisneros, Roberto; de la Barrera, Erick

2018-01-01

Atmospheric nitrogen deposition is the third largest cause of global biodiversity loss, with rates that have more than doubled over the past century. This is especially threatening for tropical regions where the deposition may soon exceed 25 kg of N ha -1 year -1 , well above the threshold for physiological damage of 12-20 kg of N ha -1 year -1 , depending on plant species and nitrogenous compound. It is thus urgent to monitor these regions where the most diverse biotas occur. However, most studies have been conducted in Europe, the USA and recently in China. This review presents the case for the potential use of biological organisms to monitor nitrogen deposition, with emphasis on tropical plants. We first present an overview of atmospheric chemistry and the nitrogen metabolism of potential biomonitors, followed by a framework for monitoring nitrogen deposition based on the simultaneous use of various functional groups. In particular, the tissue nitrogen content responds to the rate of deposition, especially for mosses, whose nitrogen content increases by 1‰ per kilogram of N ha -1 year -1 . The isotopic signature, δ 15 N, is a useful indicator of the nitrogen source, as the slightly negative values (e.g. 5‰) of plants from natural environments can become very negative (-11.2‰) in sites with agricultural and husbandry activities, but very positive (13.3‰) in urban environments with high vehicular activity. Mosses are good biomonitors for wet deposition and atmospheric epiphytes for dry deposition. In turn, the nitrogen saturation of ecosystems can be monitored with trees whose isotopic values increase with saturation. Although given ecophysiological limitations of different organisms, particular studies should be conducted in each area of interest to determine the most suitable biomonitors. Overall, biomonitors can provide an integrative approach for characterizing nitrogen deposition in regions where the deployment of automated instruments or passive monitoring is not feasible or can be complementary.

Biomonitors of atmospheric nitrogen deposition: potential uses and limitations

PubMed Central

Díaz-Álvarez, Edison A; Lindig-Cisneros, Roberto

2018-01-01

Abstract Atmospheric nitrogen deposition is the third largest cause of global biodiversity loss, with rates that have more than doubled over the past century. This is especially threatening for tropical regions where the deposition may soon exceed 25 kg of N ha−1 year−1, well above the threshold for physiological damage of 12–20 kg of N ha−1 year−1, depending on plant species and nitrogenous compound. It is thus urgent to monitor these regions where the most diverse biotas occur. However, most studies have been conducted in Europe, the USA and recently in China. This review presents the case for the potential use of biological organisms to monitor nitrogen deposition, with emphasis on tropical plants. We first present an overview of atmospheric chemistry and the nitrogen metabolism of potential biomonitors, followed by a framework for monitoring nitrogen deposition based on the simultaneous use of various functional groups. In particular, the tissue nitrogen content responds to the rate of deposition, especially for mosses, whose nitrogen content increases by 1‰ per kilogram of N ha−1 year−1. The isotopic signature, δ15N, is a useful indicator of the nitrogen source, as the slightly negative values (e.g. 5‰) of plants from natural environments can become very negative (−11.2‰) in sites with agricultural and husbandry activities, but very positive (13.3‰) in urban environments with high vehicular activity. Mosses are good biomonitors for wet deposition and atmospheric epiphytes for dry deposition. In turn, the nitrogen saturation of ecosystems can be monitored with trees whose isotopic values increase with saturation. Although given ecophysiological limitations of different organisms, particular studies should be conducted in each area of interest to determine the most suitable biomonitors. Overall, biomonitors can provide an integrative approach for characterizing nitrogen deposition in regions where the deployment of automated instruments or passive monitoring is not feasible or can be complementary. PMID:29564134

Run-Time Support for Rapid Prototyping

DTIC Science & Technology

1988-12-01

prototyping. One such system is the Computer-Aided Proto- typing System (CAPS). It combines rapid prototypng with automatic program generation. Some of the...a design database, and a design management system [Ref. 3:p. 66. By using both rapid prototyping and automatic program genera- tion. CAPS will be...Most proto- typing systems perform these functions. CAPS is different in that it combines rapid prototyping with a variant of automatic program

The Perils of Prototyping.

ERIC Educational Resources Information Center

Lowry, Christina; Little, Robert

1985-01-01

The benefits of prototyping as a basis for system design include better specifications, earlier discovery of omissions and extensions, and the likelihood of salvaging much of the effort expended on the prototype. Risks and methods of prototyping during rapid systems development are also noted. (Author/MLW)

Prototype learning and dissociable categorization systems in Alzheimer's disease.

PubMed

Heindel, William C; Festa, Elena K; Ott, Brian R; Landy, Kelly M; Salmon, David P

2013-08-01

Recent neuroimaging studies suggest that prototype learning may be mediated by at least two dissociable memory systems depending on the mode of acquisition, with A/Not-A prototype learning dependent upon a perceptual representation system located within posterior visual cortex and A/B prototype learning dependent upon a declarative memory system associated with medial temporal and frontal regions. The degree to which patients with Alzheimer's disease (AD) can acquire new categorical information may therefore critically depend upon the mode of acquisition. The present study examined A/Not-A and A/B prototype learning in AD patients using procedures that allowed direct comparison of learning across tasks. Despite impaired explicit recall of category features in all tasks, patients showed differential patterns of category acquisition across tasks. First, AD patients demonstrated impaired prototype induction along with intact exemplar classification under incidental A/Not-A conditions, suggesting that the loss of functional connectivity within visual cortical areas disrupted the integration processes supporting prototype induction within the perceptual representation system. Second, AD patients demonstrated intact prototype induction but impaired exemplar classification during A/B learning under observational conditions, suggesting that this form of prototype learning is dependent on a declarative memory system that is disrupted in AD. Third, the surprisingly intact classification of both prototypes and exemplars during A/B learning under trial-and-error feedback conditions suggests that AD patients shifted control from their deficient declarative memory system to a feedback-dependent procedural memory system when training conditions allowed. Taken together, these findings serve to not only increase our understanding of category learning in AD, but to also provide new insights into the ways in which different memory systems interact to support the acquisition of categorical knowledge. Copyright © 2013 Elsevier Ltd. All rights reserved.

Prototype Learning and Dissociable Categorization Systems in Alzheimer’s Disease

PubMed Central

Heindel, William C.; Festa, Elena K.; Ott, Brian R.; Landy, Kelly M.; Salmon, David P.

2015-01-01

Recent neuroimaging studies suggest that prototype learning may be mediated by at least two dissociable memory systems depending on the mode of acquisition, with A/Not-A prototype learning dependent upon a perceptual representation system located within posterior visual cortex and A/B prototype learning dependent upon a declarative memory system associated with medial temporal and frontal regions. The degree to which patients with Alzheimer’s disease (AD) can acquire new categorical information may therefore critically depend upon the mode of acquisition. The present study examined A/Not-A and A/B prototype learning in AD patients using procedures that allowed direct comparison of learning across tasks. Despite impaired explicit recall of category features in all tasks, patients showed differential patterns of category acquisition across tasks. First, AD patients demonstrated impaired prototype induction along with intact exemplar classification under incidental A/Not-A conditions, suggesting that the loss of functional connectivity within visual cortical areas disrupted the integration processes supporting prototype induction within the perceptual representation system. Second, AD patients demonstrated intact prototype induction but impaired exemplar classification during A/B learning under observational conditions, suggesting that this form of prototype learning is dependent on a declarative memory system that is disrupted in AD. Third, the surprisingly intact classification of both prototypes and exemplars during A/B learning under trial-and-error feedback conditions suggests that AD patients shifted control from their deficient declarative memory system to a feedback-dependent procedural memory system when training conditions allowed. Taken together, these findings serve to not only increase our understanding of category learning in AD, but to also provide new insights into the ways in which different memory systems interact to support the acquisition of categorical knowledge. PMID:23751172

PERTS: A Prototyping Environment for Real-Time Systems

NASA Technical Reports Server (NTRS)

Liu, Jane W. S.; Lin, Kwei-Jay; Liu, C. L.

1991-01-01

We discuss an ongoing project to build a Prototyping Environment for Real-Time Systems, called PERTS. PERTS is a unique prototyping environment in that it has (1) tools and performance models for the analysis and evaluation of real-time prototype systems, (2) building blocks for flexible real-time programs and the support system software, (3) basic building blocks of distributed and intelligent real time applications, and (4) an execution environment. PERTS will make the recent and future theoretical advances in real-time system design and engineering readily usable to practitioners. In particular, it will provide an environment for the use and evaluation of new design approaches, for experimentation with alternative system building blocks and for the analysis and performance profiling of prototype real-time systems.

Photographic images captured while sampling for bald eagles near the Davis Pond freshwater diversion structure in Barataria Bay, Louisiana (2009-10)

USGS Publications Warehouse

Jenkins, Jill A.; Jeske, Clinton W.; Allain, Larry K.

2011-01-01

The implementation of freshwater diversions in large-scale coastal restoration schemes presents several scientific and management considerations. Large-scale environmental restructuring necessitates aquatic biomonitoring, and during such field studies, photographs that document animals and habitat may be captured. Among the biomonitoring studies performed in conjunction with the Davis Pond freshwater diversion structure south of New Orleans, Louisiana, only postdiversion study images are readily available, and these are presented here.

REAL-TIME MONITORING FOR TOXICITY CAUSED BY ...

EPA Pesticide Factsheets

This project, sponsored by EPA's Environmental Monitoring for Public Access and Community Tracking (EMPACT) program, evaluated the ability of an automated biological monitoring system that measures fish ventilatory responses (ventilatory rate, ventilatory depth, and cough rate) to detect developing toxic conditions in water.In laboratory tests, acutely toxic levels of both brevetoxin (PbTx-2) and toxic Pfiesteria piscicida cultures caused fish responses primarily through large increases in cough rate. In the field, the automated biomonitoring system operated continuously for 3 months on the Chicamacomico River, a tributary to the Chesapeake Bay that has had a history of intermittent toxic algal blooms. Data gathered through this effort complemented chemical monitoring data collected by the Maryland Department of Natural Resources (DNR) as part of their Pfiesteria monitoring program. After evaluation by DNR personnel, the public could access the data on the DNR Internet web site at www.dnr.state.md.us/bay/pfiesteria/00results.html or receive more detailed information at www.aquaticpath.umd.edu/empact.. The field biomonitor identified five fish response events. Increased conductivity combined with a substantial decrease in water temperature was the likely cause of one event, while contaminants (probably surfactants) released from inadequately rinsed particle filters produced another response. The other three events, characterized by greatly increased cough ra

Software engineering activities at SEI (Software Engineering Institute)

NASA Technical Reports Server (NTRS)

Chittister, Clyde

1990-01-01

Prototyping was shown to ease system specification and implementation, especially in the area of user interfaces. Other prototyping approaches do not allow for the evolution of the prototype into a production system or support maintenance after the system is fielded. A set of goals is presented for a modern user interface environment and Serpent, a prototype implementation that achieves these goals, is described.

Development of a Non-Invasive Biomonitoring Approach to Determine Exposure to the Organophosphorus Insecticide Chlorpyrifos in Rat Saliva

DOE Office of Scientific and Technical Information (OSTI.GOV)

Timchalk, Chuck; Campbell, James A.; Liu, Guodong

2007-03-01

Abstract Non-invasive biomonitoring approaches are being developed using reliable portable analytical systems to quantify dosimetry utilizing readily obtainable body fluids, such as saliva. In the current study, rats were given single oral gavage doses (1, 10 or 50 mg/kg) of the insecticide chlorpyrifos (CPF), saliva and blood were collected from groups of animals (4/time-point) at 3, 6, and 12 hr post-dosing, and the samples were analyzed for the CPF metabolite trichlorpyridinol (TCP). Trichlorpyridinol was detected in both blood and saliva at all doses and the TCP concentration in blood exceeded saliva, although the kinetics in blood and saliva were comparable.more » A physiologically based pharmacokinetic and pharmacodynamic (PBPK/PD) model for CPF incorporated a compartment model to describe the time-course of TCP in blood and saliva. The model adequately simulated the experimental results over the dose ranges evaluated. A rapid and sensitive sequential injection (SI) electrochemical immunoassay was developed to monitor TCP, and the reported detection limit for TCP in water was 6 ng/L. Computer model simulation in the range of the Allowable Daily Intake (ADI) or Reference Dose (RfD) for CPF (0.01-0.003 mg/kg/day) suggest that the electrochemical immunoassay had adequate sensitivity to detect and quantify TCP in saliva at these low exposure levels. To validate this approach further studies are needed to more fully understand the pharmacokinetics of CPF and TCP excretion in saliva. The utilization of saliva as a biomonitoring matrix, coupled to real-time quantitation and PBPK/PD modeling represents a novel approach with broad application for evaluating both occupational and environmental exposures to insecticides.« less

Post-Remediation Biomonitoring of Pesticides in Marine Waters Near the United Heckathorn Site, Richmond, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

Antrim, Liam D.; Kohn, Nancy P.

Marine sediment remediation at the United Heckathorn Superfund Site was completed in April 1997. Water and mussel tissues were sampled in January 1998 from four stations near Lauritzen Canal in Richmond, California, for the first post-remediation monitoring of marine areas near the United Heckathorn Site. Dieldrin and DDT were analyzed in water samples, tissue samples from resident mussels, and tissue samples from transplanted mussels deployed for 4 months. Concentrations of dieldrin and total DDT in water and total DDT in tissue were compared to pre-remediation data available from the California State Mussel Watch program (tissues) and the Ecological Risk Assessmentmore » for the United Heckathorn Superfund Site (tissues and water). Biomonitoring results indicated that pesticides were still bioavailable in the water column, and have not been reduced from pre-remediation levels. Annual biomonitoring will continue to assess the effectiveness of remedial actions at the United Heckathorn Site.« less

Assessment of human health risk related to metals by the use of biomonitors in the province of Córdoba, Argentina.

PubMed

Carreras, Hebe A; Wannaz, Eduardo D; Pignata, María L

2009-01-01

The evaluation of metal contents in the environment is of vital importance for the assessment of human exposure. Thus the species Usnea amblyoclada, Ramalina celastri and Tillandsia capillaris were tested as bioaccumulators of transition metals in the urban area of Córdoba city, Argentina. The level of metals on biomonitors was compared to that of total deposition samples. All three species discriminated zones within the urban area of Córdoba city with different pollution levels; they revealed high levels of Zn in the downtown area and confirmed high levels of some transition metals in an industrial area. The correlation analysis revealed that the lichen R. celastri had the highest correlation rates with total deposition samples, suggesting it is a valuable biomonitor of atmospheric pollution. A significant relationship was also observed between respiratory diseases in children and the contents of metal accumulated in R. celastri and T. capillaris, indicating their usefulness when assessing human exposure to metals.

Snapping turtles (Chelydra serpentina) as biomonitors of lead contamination of the Big River in Missouri`s Old Lead Belt

DOE Office of Scientific and Technical Information (OSTI.GOV)

Overmann, S.R.; Krajicek, J.J.

1995-04-01

The usefulness of common snapping turtles (Chelydra serpentina) as biomonitors of lead (Pb) contamination of aquatic ecosystems was assessed. Thirty-seven snapping turtles were collected from three sites on the Big River, an Ozarkian stream contaminated with Pb mine tailings. Morphometric measurements, tissue Pb concentrations (muscle, blood, bone, carapace, brain, and liver), {delta}-aminolevulinic acid dehydratase ({delta}-ALAD) activity, hematocrit, hemoglobin, plasma glucose, osmolality, and chloride ion content were measured. The data showed no effects of Pb contamination on capture success or morphological measurements. Tissue Pb concentrations were related to capture location. Hematocrit, plasma osmolality, plasma glucose, and plasma chloride ion content weremore » not significantly different with respect to capture location. The {delta}-ALAD activity levels were decreased in turtles taken from contaminated sites. Lead levels in the Big River do not appear to be adversely affecting the snapping turtles of the river. Chelydra serpentina is a useful species for biomonitoring of Pb-contaminated aquatic environments.« less

Leaves of Phragmites australis as potential atmospheric biomonitors of Platinum Group Elements.

PubMed

Bonanno, Giuseppe; Pavone, Pietro

2015-04-01

The increasing emissions of Platinum Group Elements (PGEs), namely Pt, Pd and Rh, may pose a significant risk to ecosystem processes and human health. A periodic assessment of PGEs distribution in the environment is thus of the utmost importance for the implementation of timely measures of mitigation. Although several studies have quantified PGEs in different life forms such as mammals, birds, fish, crustaceans, algae, mosses and even human beings, data about vascular plants need further surveys. This study aimed to test the suitability of the grass Phragmites australis (common reed) as a biomonitor of PGEs atmospheric pollution. The results showed that Pd and Pt concentrations in leaves are significantly higher in urban areas. In particular, Pd showed the highest range of values in line with current studies that consider palladium as the main element of traffic-related pollution. Overall, the leaves of Phragmites australis reflected the different gradient of PGEs emissions, and may thus be considered as potential biomonitors of atmospheric pollution. Copyright © 2015 Elsevier Inc. All rights reserved.

Virtual prototyping and testing of in-vehicle interfaces.

PubMed

Bullinger, Hans-Jörg; Dangelmaier, Manfred

2003-01-15

Electronic innovations that are slowly but surely changing the very nature of driving need to be tested before being introduced to the market. To meet this need a system for integrated virtual prototyping and testing has been developed. Functional virtual prototypes of various traffic systems, such as driver assistance, driver information, and multimedia systems can now be easily tested in a driving simulator by a rapid prototyping approach. The system has been applied in recent R&D projects.

From prototype to production system: lessons learned from the evolution of the SignOut System at Mount Sinai Medical Center.

PubMed

Kushniruk, Andre; Karson, Tom; Moore, Carlton; Kannry, Joseph

2003-01-01

Approaches to the development of information systems in large health care institutions range from prototyping to conventional development of large scale production systems. This paper discusses the development of the SignOut System at Mount Sinai Medical Center, which was designed in 1997 to capture vital resident information. Local need quickly outstripped proposed delays for building a production system and a prototype system quickly became a production system. By the end of 2002 the New SignOut System was built to create an integrated application that was a true production system. In this paper we discuss the design and implementation issues in moving from a prototype to a production system. The production system had a number of advantages, including increased organizational visibility, integration into enterprise resource planning and full time staff for support. However, the prototype allowed for more rapid design and subsequent changes, less training, and equal to or superior help desk support. It is argued that healthcare IT systems may need characteristics of both prototype and production system development to rapidly meet the changing and different needs of healthcare user populations.

Urinary Phthalate Concentrations in Mothers and Their Children in Ireland: Results of the DEMOCOPHES Human Biomonitoring Study

PubMed Central

Cullen, Elizabeth; Evans, David; Griffin, Chris; Burke, Padraig; Mannion, Rory; Burns, Damien; Flanagan, Andrew; Kellegher, Ann; Schoeters, Greet; Govarts, Eva; Biot, Pierre; Casteleyn, Ludwine; Castaño, Argelia; Kolossa-Gehring, Marike; Esteban, Marta; Schwedler, Gerda; Angerer, Jürgen; Knudsen, Lisbeth E.; Joas, Reinhard; Joas, Anke; Dumez, Birgit; Sepai, Ovnair; Exley, Karen; Aerts, Dominique

2017-01-01

Background: Phthalates are chemicals which are widespread in the environment. Although the impacts on health of such exposure are unclear, there is evidence of a possible impact on the incidence of a diverse range of diseases. Monitoring of human exposure to phthalates is therefore important. This study aimed to determine the extent of phthalate exposure among mothers and their children in both rural and urban areas in Ireland, and to identify factors associated with elevated concentrations. It formed part of the ‘Demonstration of a study to Co-ordinate and Perform Human Biomonitoring on a European Scale’ (DEMOCOPHES) pilot biomonitoring study. Methods: the concentration of phthalate metabolites were determined from a convenience sample of 120 mother/child pairs. The median age of the children was 8 years. A questionnaire was used to collect information regarding lifestyle and environmental conditions of the children and mothers. Rigorous quality assurance within DEMOCOPHES guaranteed the accuracy and international comparability of results. Results: Phthalate metabolites were detected in all of the samples from both children and mothers. Concentrations were significantly higher in respondents from families with lower educational attainment and in those exposed to such items as polyvinyl chloride (PVC), fast food and personal care products (PCP). Conclusions: The study demonstrates that human biomonitoring for assessing exposure to phthalates can be undertaken in Ireland and that the exposure of the population is widespread. Further work will be necessary before the consequences of this exposure are understood. PMID:29186834

Urinary Phthalate Concentrations in Mothers and Their Children in Ireland: Results of the DEMOCOPHES Human Biomonitoring Study.

PubMed

Cullen, Elizabeth; Evans, David; Griffin, Chris; Burke, Padraig; Mannion, Rory; Burns, Damien; Flanagan, Andrew; Kellegher, Ann; Schoeters, Greet; Govarts, Eva; Biot, Pierre; Casteleyn, Ludwine; Castaño, Argelia; Kolossa-Gehring, Marike; Esteban, Marta; Schwedler, Gerda; Koch, Holger M; Angerer, Jürgen; Knudsen, Lisbeth E; Joas, Reinhard; Joas, Anke; Dumez, Birgit; Sepai, Ovnair; Exley, Karen; Aerts, Dominique

2017-11-25

Background : Phthalates are chemicals which are widespread in the environment. Although the impacts on health of such exposure are unclear, there is evidence of a possible impact on the incidence of a diverse range of diseases. Monitoring of human exposure to phthalates is therefore important. This study aimed to determine the extent of phthalate exposure among mothers and their children in both rural and urban areas in Ireland, and to identify factors associated with elevated concentrations. It formed part of the 'Demonstration of a study to Co-ordinate and Perform Human Biomonitoring on a European Scale' (DEMOCOPHES) pilot biomonitoring study. Methods : the concentration of phthalate metabolites were determined from a convenience sample of 120 mother/child pairs. The median age of the children was 8 years. A questionnaire was used to collect information regarding lifestyle and environmental conditions of the children and mothers. Rigorous quality assurance within DEMOCOPHES guaranteed the accuracy and international comparability of results. Results : Phthalate metabolites were detected in all of the samples from both children and mothers. Concentrations were significantly higher in respondents from families with lower educational attainment and in those exposed to such items as polyvinyl chloride (PVC), fast food and personal care products (PCP). Conclusions : The study demonstrates that human biomonitoring for assessing exposure to phthalates can be undertaken in Ireland and that the exposure of the population is widespread. Further work will be necessary before the consequences of this exposure are understood.

Biomonitoring of intermittent rivers and ephemeral streams in Europe: Current practice and priorities to enhance ecological status assessments.

PubMed

Stubbington, Rachel; Chadd, Richard; Cid, Núria; Csabai, Zoltán; Miliša, Marko; Morais, Manuela; Munné, Antoni; Pařil, Petr; Pešić, Vladimir; Tziortzis, Iakovos; Verdonschot, Ralf C M; Datry, Thibault

2018-03-15

Intermittent rivers and ephemeral streams (IRES) are common across Europe and dominate some Mediterranean river networks. In all climate zones, IRES support high biodiversity and provide ecosystem services. As dynamic ecosystems that transition between flowing, pool, and dry states, IRES are typically poorly represented in biomonitoring programmes implemented to characterize EU Water Framework Directive ecological status. We report the results of a survey completed by representatives from 20 European countries to identify current challenges to IRES status assessment, examples of best practice, and priorities for future research. We identify five major barriers to effective ecological status classification in IRES: 1. the exclusion of IRES from Water Framework Directive biomonitoring based on their small catchment size; 2. the lack of river typologies that distinguish between contrasting IRES; 3. difficulties in defining the 'reference conditions' that represent unimpacted dynamic ecosystems; 4. classification of IRES ecological status based on lotic communities sampled using methods developed for perennial rivers; and 5. a reliance on taxonomic characterization of local communities. Despite these challenges, we recognize examples of innovative practice that can inform modification of current biomonitoring activity to promote effective IRES status classification. Priorities for future research include reconceptualization of the reference condition approach to accommodate spatiotemporal fluctuations in community composition, and modification of indices of ecosystem health to recognize both taxon-specific sensitivities to intermittence and dispersal abilities, within a landscape context. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Intra-urban biomonitoring: Source apportionment using tree barks to identify air pollution sources.

PubMed

Moreira, Tiana Carla Lopes; de Oliveira, Regiani Carvalho; Amato, Luís Fernando Lourenço; Kang, Choong-Min; Saldiva, Paulo Hilário Nascimento; Saiki, Mitiko

2016-05-01

It is of great interest to evaluate if there is a relationship between possible sources and trace elements using biomonitoring techniques. In this study, tree bark samples of 171 trees were collected using a biomonitoring technique in the inner city of São Paulo. The trace elements (Al, Ba, Ca, Cl, Cu, Fe, K, Mg, Mn, Na, P, Rb, S, Sr and Zn) were determined by the energy dispersive X-ray fluorescence (EDXRF) spectrometry. The Principal Component Analysis (PCA) was applied to identify the plausible sources associated with tree bark measurements. The greatest source was vehicle-induced non-tailpipe emissions derived mainly from brakes and tires wear-out and road dust resuspension (characterized with Al, Ba, Cu, Fe, Mn and Zn), which was explained by 27.1% of the variance, followed by cement (14.8%), sea salt (11.6%) and biomass burning (10%), and fossil fuel combustion (9.8%). We also verified that the elements related to vehicular emission showed different concentrations at different sites of the same street, which might be helpful for a new street classification according to the emission source. The spatial distribution maps of element concentrations were obtained to evaluate the different levels of pollution in streets and avenues. Results indicated that biomonitoring techniques using tree bark can be applied to evaluate dispersion of air pollution and provide reliable data for the further epidemiological studies. Copyright © 2016 Elsevier Ltd. All rights reserved.

Application of the BMWP-Costa Rica biotic index in aquatic biomonitoring: sensitivity to collection method and sampling intensity.

PubMed

Gutiérrez-Fonseca, Pablo E; Lorion, Christopher M

2014-04-01

The use of aquatic macroinvertebrates as bio-indicators in water quality studies has increased considerably over the last decade in Costa Rica, and standard biomonitoring methods have now been formulated at the national level. Nevertheless, questions remain about the effectiveness of different methods of sampling freshwater benthic assemblages, and how sampling intensity may influence biomonitoring results. In this study, we compared the results of qualitative sampling using commonly applied methods with a more intensive quantitative approach at 12 sites in small, lowland streams on the southern Caribbean slope of Costa Rica. Qualitative samples were collected following the official protocol using a strainer during a set time period and macroinvertebrates were field-picked. Quantitative sampling involved collecting ten replicate Surber samples and picking out macroinvertebrates in the laboratory with a stereomicroscope. The strainer sampling method consistently yielded fewer individuals and families than quantitative samples. As a result, site scores calculated using the Biological Monitoring Working Party-Costa Rica (BMWP-CR) biotic index often differed greatly depending on the sampling method. Site water quality classifications using the BMWP-CR index differed between the two sampling methods for 11 of the 12 sites in 2005, and for 9 of the 12 sites in 2006. Sampling intensity clearly had a strong influence on BMWP-CR index scores, as well as perceived differences between reference and impacted sites. Achieving reliable and consistent biomonitoring results for lowland Costa Rican streams may demand intensive sampling and requires careful consideration of sampling methods.

Software Prototyping: Designing Systems for Users.

ERIC Educational Resources Information Center

Spies, Phyllis Bova

1983-01-01

Reports on major change in computer software development process--the prototype model, i.e., implementation of skeletal system that is enhanced during interaction with users. Expensive and unreliable software, software design errors, traditional development approach, resources required for prototyping, success stories, and systems designer's role…

Design of Smart Home Systems Prototype Using MyRIO

NASA Astrophysics Data System (ADS)

Ratna Wati, Dwi Ann; Abadianto, Dika

2017-06-01

This paper presents the design of smart home systems prototype. It applies. MyRIO 1900 embedded device as the main controller of the smart home systems. The systems include wireless monitoring systems and email based notifications as well as data logging. The prototype systems use simulated sensor such as temperature sensor, push button as proximity sensor, and keypad while its simulated actuators are buzzer as alarm system, LED as light and LCD. Based on the test and analysis, the smart home systems prototype as well as the wireless monitoring systems have real time responses when input signals are available. Tbe performance of MyRIO controller is excellent and it results in a stable system.

A Residual Chlorine Removal Method to Allow Drinking Water Monitoring by Biological Early Warning Systems

DTIC Science & Technology

2005-03-18

Lepomis macrochirus). have been disinfected with chloramines The threshold for a toxicity alarm by was not determined. Biomonitor users the USACEHR...residual solution. This caused an elevated chlorine from water disinfected with chlorine level (measured at 0.08 FRC) chloramines . In testing with a rapid...have been disinfected A Handbook, Volume 2, 8. Kluwer with chloramines . Academic/Plenum Publishers, New York, NY, pp 123-141. 5. Recommendations

System requirements for a computerised patient record information system at a busy primary health care clinic.

PubMed

Blignaut, P J; McDonald, T; Tolmie, C J

2001-05-01

A prototyping approach was used to determine the essential system requirements of a computerised patient record information system for a typical township primary health care clinic. A pilot clinic was identified and the existing manual system and business processes in this clinic was studied intensively before the first prototype was implemented. Interviews with users, incidental observations and analysis of actual data entered were used as primary techniques to refine the prototype system iteratively until a system with an acceptable data set and adequate functionalities were in place. Several non-functional and user-related requirements were also discovered during the prototyping period.

Real-time contaminant sensing and control in civil infrastructure systems

NASA Astrophysics Data System (ADS)

Rimer, Sara; Katopodes, Nikolaos

2014-11-01

A laboratory-scale prototype has been designed and implemented to test the feasibility of real-time contaminant sensing and control in civil infrastructure systems. A blower wind tunnel is the basis of the prototype design, with propylene glycol smoke as the ``contaminant.'' A camera sensor and compressed-air vacuum nozzle system is set up at the test section portion of the prototype to visually sense and then control the contaminant; a real-time controller is programmed to read in data from the camera sensor and administer pressure to regulators controlling the compressed air operating the vacuum nozzles. A computational fluid dynamics model is being integrated in with this prototype to inform the correct pressure to supply to the regulators in order to optimally control the contaminant's removal from the prototype. The performance of the prototype has been evaluated against the computational fluid dynamics model and is discussed in this presentation. Furthermore, the initial performance of the sensor-control system implemented in the test section of the prototype is discussed. NSF-CMMI 0856438.

Suitability of virtual prototypes to support human factors/ergonomics evaluation during the design.

PubMed

Aromaa, Susanna; Väänänen, Kaisa

2016-09-01

In recent years, the use of virtual prototyping has increased in product development processes, especially in the assessment of complex systems targeted at end-users. The purpose of this study was to evaluate the suitability of virtual prototyping to support human factors/ergonomics evaluation (HFE) during the design phase. Two different virtual prototypes were used: augmented reality (AR) and virtual environment (VE) prototypes of a maintenance platform of a rock crushing machine. Nineteen designers and other stakeholders were asked to assess the suitability of the prototype for HFE evaluation. Results indicate that the system model characteristics and user interface affect the experienced suitability. The VE system was valued as being more suitable to support the assessment of visibility, reach, and the use of tools than the AR system. The findings of this study can be used as a guidance for the implementing virtual prototypes in the product development process. Copyright © 2016 Elsevier Ltd. All rights reserved.

Optimal wavelet denoising for smart biomonitor systems

NASA Astrophysics Data System (ADS)

Messer, Sheila R.; Agzarian, John; Abbott, Derek

2001-03-01

Future smart-systems promise many benefits for biomedical diagnostics. The ideal is for simple portable systems that display and interpret information from smart integrated probes or MEMS-based devices. In this paper, we will discuss a step towards this vision with a heart bio-monitor case study. An electronic stethoscope is used to record heart sounds and the problem of extracting noise from the signal is addressed via the use of wavelets and averaging. In our example of heartbeat analysis, phonocardiograms (PCGs) have many advantages in that they may be replayed and analysed for spectral and frequency information. Many sources of noise may pollute a PCG including foetal breath sounds if the subject is pregnant, lung and breath sounds, environmental noise and noise from contact between the recording device and the skin. Wavelets can be employed to denoise the PCG. The signal is decomposed by a discrete wavelet transform. Due to the efficient decomposition of heart signals, their wavelet coefficients tend to be much larger than those due to noise. Thus, coefficients below a certain level are regarded as noise and are thresholded out. The signal can then be reconstructed without significant loss of information in the signal. The questions that this study attempts to answer are which wavelet families, levels of decomposition, and thresholding techniques best remove the noise in a PCG. The use of averaging in combination with wavelet denoising is also addressed. Possible applications of the Hilbert Transform to heart sound analysis are discussed.

A diagnostic prototype of the potable water subsystem of the Space Station Freedom ECLSS

NASA Technical Reports Server (NTRS)

Lukefahr, Brenda D.; Rochowiak, Daniel M.; Benson, Brian L.; Rogers, John S.; Mckee, James W.

1989-01-01

In analyzing the baseline Environmental Control and Life Support System (ECLSS) command and control architecture, various processes are found which would be enhanced by the use of knowledge based system methods of implementation. The most suitable process for prototyping using rule based methods are documented, while domain knowledge resources and other practical considerations are examined. Requirements for a prototype rule based software system are documented. These requirements reflect Space Station Freedom ECLSS software and hardware development efforts, and knowledge based system requirements. A quick prototype knowledge based system environment is researched and developed.

A Multiple Sensor Machine Vision System for Automatic Hardwood Feature Detection

Treesearch

D. Earl Kline; Richard W. Conners; Daniel L. Schmoldt; Philip A. Araman; Robert L. Brisbin

1993-01-01

A multiple sensor machine vision prototype is being developed to scan full size hardwood lumber at industrial speeds for automatically detecting features such as knots holes, wane, stain, splits, checks, and color. The prototype integrates a multiple sensor imaging system, a materials handling system, a computer system, and application software. The prototype provides...

Evaluation of expert systems - An approach and case study. [of determining software functional requirements for command management of satellites

NASA Technical Reports Server (NTRS)

Liebowitz, J.

1985-01-01

Techniques that were applied in defining an expert system prototype for first-cut evaluations of the software functional requirements of NASA satellite command management activities are described. The prototype was developed using the Knowledge Engineering System. Criteria were selected for evaluating the satellite software before defining the expert system prototype. Application of the prototype system is illustrated in terms of the evaluation procedures used with the COBE satellite to be launched in 1988. The limited number of options which can be considered by the program mandates that biases in the system output must be well understood by the users.

Development of 3000 m Subsea Blowout Preventer Experimental Prototype

NASA Astrophysics Data System (ADS)

Cai, Baoping; Liu, Yonghong; Huang, Zhiqian; Ma, Yunpeng; Zhao, Yubin

2017-12-01

A subsea blowout preventer experimental prototype is developed to meet the requirement of training operators, and the prototype consists of hydraulic control system, electronic control system and small-sized blowout preventer stack. Both the hydraulic control system and the electronic system are dual-mode redundant systems. Each system works independently and is switchable when there are any malfunctions. And it significantly improves the operation reliability of the equipment.

ALLTEM Multi-Axis Electromagnetic Induction System Demonstration and Validation

DTIC Science & Technology

2012-08-01

threshold T-high higher threshold TMGS Tensor Magnetic Gradiometer System TOI target of interest Tx ALLTEM transmitter USGS U.S. Geological...the Tensor Magnetic Gradiometer System ( TMGS ) and two prototype EMI instruments, the Very Early Time-domain ElectroMagnetic (VETEM) system and the...project one prototype magnetic system, the TMGS , and two prototype EMI instruments, VETEM and the High Frequency Sounder, were evaluated. Subsequent

Steamer II: Steamer prototype component inventory and user interface commands. Technical report, 1988-1989

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dickieson, J.L.; Thode, W.F.; Newbury, K.

1988-12-01

Over the last several years, Navy Personnel Research and Development has produced a prototype simulation of a 1200-psi steam plant. This simulation, called Steamer, is installed on an expensive Symbolics minicomputer at the Surface Warfare Officers School, Pacific Coronado, California. The fundamental research goal of the Steamer prototype system was to evaluate the potential of, what was then, new artificial intelligence (AI) hardware and software technology for supporting the construction of computer-based training systems using graphic representations of complex, dynamic systems. The area of propulsion engineering was chosen for a number of reasons. This document describes the Steamer prototype systemmore » components and user interface commands and establishes a starting point for designing, developing, and implementing Steamer II. Careful examination of the actual program code produced an inventory that describes the hardware, system software, application software, and documentation for the Steamer prototype system. Exercising all menu options systematically produced an inventory of all Steamer prototype user interface commands.« less

Policy recommendations and cost implications for a more sustainable framework for European human biomonitoring surveys.

PubMed

Joas, Anke; Knudsen, Lisbeth E; Kolossa-Gehring, Marike; Sepai, Ovnair; Casteleyn, Ludwine; Schoeters, Greet; Angerer, Jürgen; Castaño, Argelia; Aerts, Dominique; Biot, Pierre; Horvat, Milena; Bloemen, Louis; Reis, M Fátima; Lupsa, Ioana-Rodica; Katsonouri, Andromachi; Cerna, Milena; Berglund, Marika; Crettaz, Pierre; Rudnai, Peter; Halzlova, Katarina; Mulcahy, Maurice; Gutleb, Arno C; Fischer, Marc E; Becher, Georg; Fréry, Nadine; Jensen, Genon; Van Vliet, Lisette; Koch, Holger M; Den Hond, Elly; Fiddicke, Ulrike; Esteban, Marta; Exley, Karen; Schwedler, Gerda; Seiwert, Margarete; Ligocka, Danuta; Hohenblum, Philipp; Kyrtopoulos, Soterios; Botsivali, Maria; DeFelip, Elena; Guillou, Claude; Reniero, Fabiano; Grazuleviciene, Regina; Veidebaum, Toomas; Mørck, Thit A; Nielsen, Jeanette K S; Jensen, Janne F; Rivas, Teresa C; Sanchez, Jinny; Koppen, Gudrun; Smolders, Roel; Kozepesy, Szilvia; Hadjipanayis, Adamos; Krskova, Andrea; Mannion, Rory; Jakubowski, Marek; Fucic, J Aleksandra; Pereira-Miguel, Jose; Gurzau, Anca E; Jajcaj, Michal; Mazej, Darja; Tratnik, Janja Snoj; Lehmann, Andrea; Larsson, Kristin; Dumez, Birgit; Joas, Reinhard

2015-08-01

The potential of Human Biomonitoring (HBM) in exposure characterisation and risk assessment is well established in the scientific HBM community and regulatory arena by many publications. The European Environment and Health Strategy as well as the Environment and Health Action Plan 2004-2010 of the European Commission recognised the value of HBM and the relevance and importance of coordination of HBM programmes in Europe. Based on existing and planned HBM projects and programmes of work and capabilities in Europe the Seventh Framework Programme (FP 7) funded COPHES (COnsortium to Perform Human Biomonitoring on a European Scale) to advance and improve comparability of HBM data across Europe. The pilot study protocol was tested in 17 European countries in the DEMOCOPHES feasibility study (DEMOnstration of a study to COordinate and Perform Human biomonitoring on a European Scale) cofunded (50%) under the LIFE+ programme of the European Commission. The potential of HBM in supporting and evaluating policy making (including e.g. REACH) and in awareness raising on environmental health, should significantly advance the process towards a fully operational, continuous, sustainable and scientifically based EU HBM programme. From a number of stakeholder activities during the past 10 years and the national engagement, a framework for sustainable HBM structure in Europe is recommended involving national institutions within environment, health and food as well as European institutions such as ECHA, EEA, and EFSA. An economic frame with shared cost implications for national and European institutions is suggested benefitting from the capacity building set up by COPHES/DEMOCOPHES. Copyright © 2014 Elsevier Inc. All rights reserved.

Calculating summary statistics for population chemical biomonitoring in women of childbearing age with adjustment for age-specific natality.

PubMed

Axelrad, Daniel A; Cohen, Jonathan

2011-01-01

The effects of chemical exposures during pregnancy on children's health have been an increasing focus of environmental health research in recent years, leading to greater interest in biomonitoring of chemicals in women of childbearing age in the general population. Measurements of mercury in blood from the National Health and Nutrition Examination Survey are frequently reported for "women of childbearing age," defined to be of ages 16-49 years. The intent is to represent prenatal chemical exposure, but blood mercury levels increase with age. Furthermore, women of different ages have different probabilities of giving birth. We evaluated options to address potential bias in biomonitoring summary statistics for women of childbearing age by accounting for age-specific probabilities of giving birth. We calculated median and 95th percentile levels of mercury, PCBs, and cotinine using these approaches: option 1: women aged 16-49 years without natality adjustment; option 2: women aged 16-39 years without natality adjustment; option 3: women aged 16-49 years, adjusted for natality by age; option 4: women aged 16-49 years, adjusted for natality by age and race/ethnicity. Among the three chemicals examined, the choice of option has the greatest impact on estimated levels of serum PCBs, which are strongly associated with age. Serum cotinine levels among Black non-Hispanic women of childbearing age are understated when age-specific natality is not considered. For characterizing in utero exposures, adjustment using age-specific natality provides a substantial improvement in estimation of biomonitoring summary statistics. Copyright © 2010 Elsevier Inc. All rights reserved.

Tracking pan-continental trends in environmental contamination using sentinel raptors-what types of samples should we use?

PubMed

Espín, S; García-Fernández, A J; Herzke, D; Shore, R F; van Hattum, B; Martínez-López, E; Coeurdassier, M; Eulaers, I; Fritsch, C; Gómez-Ramírez, P; Jaspers, V L B; Krone, O; Duke, G; Helander, B; Mateo, R; Movalli, P; Sonne, C; van den Brink, N W

2016-05-01

Biomonitoring using birds of prey as sentinel species has been mooted as a way to evaluate the success of European Union directives that are designed to protect people and the environment across Europe from industrial contaminants and pesticides. No such pan-European evaluation currently exists. Coordination of such large scale monitoring would require harmonisation across multiple countries of the types of samples collected and analysed-matrices vary in the ease with which they can be collected and the information they provide. We report the first ever pan-European assessment of which raptor samples are collected across Europe and review their suitability for biomonitoring. Currently, some 182 monitoring programmes across 33 European countries collect a variety of raptor samples, and we discuss the relative merits of each for monitoring current priority and emerging compounds. Of the matrices collected, blood and liver are used most extensively for quantifying trends in recent and longer-term contaminant exposure, respectively. These matrices are potentially the most effective for pan-European biomonitoring but are not so widely and frequently collected as others. We found that failed eggs and feathers are the most widely collected samples. Because of this ubiquity, they may provide the best opportunities for widescale biomonitoring, although neither is suitable for all compounds. We advocate piloting pan-European monitoring of selected priority compounds using these matrices and developing read-across approaches to accommodate any effects that trophic pathway and species differences in accumulation may have on our ability to track environmental trends in contaminants.

Pollution gets personal! A first population-based human biomonitoring study in Austria.

PubMed

Hohenblum, Philipp; Steinbichl, Philipp; Raffesberg, Wolfgang; Weiss, Stefan; Moche, Wolfgang; Vallant, Birgit; Scharf, Sigrid; Haluza, Daniela; Moshammer, Hanns; Kundi, Michael; Piegler, Brigitte; Wallner, Peter; Hutter, Hans-Peter

2012-02-01

Humans are exposed to a broad variety of man-made chemicals. Human biomonitoring (HBM) data reveal the individual body burden irrespective of sources and routes of uptake. A first population-based study was started in Austria in 2008 and was finished at the end of May 2011. This cross sectional study aims at documenting the extent, the distribution and the determinants of human exposure to industrial chemicals as well as proving the feasibility of a representative HBM study. Overall, 150 volunteers (50 families) were selected by stratified random sampling. Exposure to phthalates, trisphosphates, polybrominated diphenyl ethers (PBDE), bisphenol A (along with nonyl- and octyl phenol) and methyl mercury was assessed. Sixteen of 18 PBDE determined were detected above the limit of quantification (LOQ) in blood samples with #153 and #197 the most abundant species. Bisphenol A in urine was measured in a subsample of 25 with only 4 samples found above the LOQ. In 3 of 100 urine samples at least one of 8 trisphosphate compounds assessed was above the LOQ. These first analytical results of the human biomonitoring data show that the body burden of the Austrian population with respect to the assessed compounds is comparable to or even lower than in other European countries. Overall, the study revealed that in order to develop a feasible protocol for representative human biomonitoring studies procedures have to be optimized to allow for non-invasive sampling of body tissues in accordance with the main metabolic pathways. Procedures of participants' recruitment were, however, labor intensive and have to be improved. Copyright © 2011 Elsevier GmbH. All rights reserved.

Benthic Foraminifera As A Novel Bio-monitoring Tool In The Assessment Of Environmental Impacts Linked To Marine Aquaculture

NASA Astrophysics Data System (ADS)

Alammar, Montaha; Austin, William

2017-04-01

The present study represents an attempt to evaluate the impacts of marine aquaculture on benthic foraminiferal communities in order to develop an improved, quantitative understanding of their response to the variation in benthic environmental gradients associated with fish farms in Scotland. Furthermore, their performance as a bio-monitoring tool will be discussed and outlined in ongoing research to evaluate their performance alongside traditional bioecological indicators. Foraminiferal faunas offer the potential to assess ecological quality status through their response to stress gradients (e.g. organic matter enrichment), such as that caused by intensive fish farming in coastal sediments. In this study, we followed the Foraminiferal Bio-Monitoring (FOBIMO) protocol (Schönfeld. et al., 2012), which proposed a standardised methodology of using foraminifera as a bio-monitoring tool to evaluate the quality of the marine ecosystem and applied these protocols to the rapidly expanding marine aquaculture sector in Scotland, UK. Eight stations were sampled along a transect in Loch Creran, west coast of Scotland, to describe the spatial and down-core (temporal) distribution pattern of benthic foraminiferal assemblages. Triplicate, Rose-Bengal stained samples from an interval of (0-1cm) below the sediment surface were studied at each station from below the fish cages (impacted stations) to a distance from the farming sites (control stations). Morphospecies counts were conducted, and the organic carbon and the grain size distributions determined. Species richness beneath these fish farming cages were analysed and showed a reduction of foraminifera density and diversity at the impacted stations.

Negative Ion Chemical Ionization Mass Spectrometry for the Analysis of 3,5,6-trichloro-2-pyridinol in Saliva of Rats Exposed to Chlorpyrifos

DOE Office of Scientific and Technical Information (OSTI.GOV)

Campbell, James A.; Timchalk, Chuck; Kousba, Ahmed A.

2005-05-01

Organophosphorus (OP) insecticides (e.g. chlorpyrifos) are widely used in a variety of applications, and the potential exists for significant occupational and environmental exposures. They have been associated with more occupational poisoning cases than any other class of insecticides. One of the best approaches for accurately assessing human dosimetry and determining risk from both occupational and environmental exposure is biomonitoring. Biological matrices such as blood and urine have been routinely used for biomonitoring; however, other matrices such as saliva represent a simple and readily obtainable fluid. As a result, saliva has been suggested as an alternative biological matrix for the evaluationmore » of a broad range of biomarkers such as environmental contaminants, drugs of abuse, hormones, chemotherapeutics, heavy metals, and pesticides. Chlorpyrifos (CPF), and its major metabolite, 3,5,6-trichloro-2-pyridinol (TCP), have been quantified in urine and blood as a biomarker for exposure to OP insecticides. The purpose of this study was to develop an analytical approach for detecting and quantitating the levels of TCP in saliva obtained from rats exposed to CPF and to evaluate the potential of saliva as a non-invasive biomonitoring matrix. Adult male rats were administered CPF, and blood and saliva were humanely collected for analysis of TCP and CPF. TCP was detected and quantitated in saliva using negative ion chemical ionization mass spectrometry with selected ion monitoring. Initial results indicate that saliva may be potentially utilized as a non-invasive biomonitoring matrix to determine exposure to organophosphate insecticides.« less

Application of an Automated Fish Biomonitoring System at Old O-Field for Continuous Acute Toxicity Effluent Monitoring

DTIC Science & Technology

1996-05-01

Tommy R. Shedd, Henry S. Gardner , Robert A. Finch, and Robert C. Bishoff U.S. Army Biomedical Research and Development Laboratory Fort Detrick...Zeigler Bros., Inc., Gardners , PA) plus frozen brine shrimp (Living World, Virginia Pet Supply, Fairfax VA). Fish were not fed during the 2-week...Environmental Protection Agency, Washington, DC. 3. Diamond , J.M. and T.R.Shedd. 1995. Standard Guide for Ventilatory Behavioral Toxicology Testing

Bivalve Omics: State of the Art and Potential Applications for the Biomonitoring of Harmful Marine Compounds

PubMed Central

Suárez-Ulloa, Victoria; Fernández-Tajes, Juan; Manfrin, Chiara; Gerdol, Marco; Venier, Paola; Eirín-López, José M.

2013-01-01

The extraordinary progress experienced by sequencing technologies and bioinformatics has made the development of omic studies virtually ubiquitous in all fields of life sciences nowadays. However, scientific attention has been quite unevenly distributed throughout the different branches of the tree of life, leaving molluscs, one of the most diverse animal groups, relatively unexplored and without representation within the narrow collection of well established model organisms. Within this Phylum, bivalve molluscs play a fundamental role in the functioning of the marine ecosystem, constitute very valuable commercial resources in aquaculture, and have been widely used as sentinel organisms in the biomonitoring of marine pollution. Yet, it has only been very recently that this complex group of organisms became a preferential subject for omic studies, posing new challenges for their integrative characterization. The present contribution aims to give a detailed insight into the state of the art of the omic studies and functional information analysis of bivalve molluscs, providing a timely perspective on the available data resources and on the current and prospective applications for the biomonitoring of harmful marine compounds. PMID:24189277

Feasibility study of feces for noninvasive biomonitoring of brominated flame retardants in toddlers.

PubMed

Sahlström, Leena M O; Sellström, Ulla; de Wit, Cynthia A; Lignell, Sanna; Darnerud, Per Ola

2015-01-06

This study investigated the feasibility of using feces as a noninvasive matrix to estimate serum concentrations of brominated flame retardants (BFRs) in toddlers for biomonitoring purposes. Tri- to decabrominated diphenyl ethers (tri-decaBDEs), isomer-specific hexabromocyclododecanes, and 16 emerging BFRs were determined in feces from 22 toddlers (11-15 months of age), and results were compared to previously analyzed matched serum samples. BDE-47, -153, -196, -197, -203, -206, -207, -208, and -209 were detected in the feces creating a matched data set (feces-serum, n = 21). Tetra-octaBDE concentrations were significantly higher (Student's paired comparisons t test, α = 0.05) in serum versus feces with BDE-153 having the highest mean difference between the sample matrices. BDE-209 was found in significantly higher concentrations in feces compared to serum. Significant correlations (Pearson's, α = 0.05) between congener-specific concentrations in feces and serum were found for all BDEs except BDE-197 and -203. The feces-serum associations found can be used to estimate serum concentrations of tetra-decaBDEs from feces concentrations and enable a noninvasive sampling method for biomonitoring BDEs in toddlers.

A biomonitor for tracking changes in the availability of lakewater cadmium over space and time

USGS Publications Warehouse

Hare, L.; Tessier, A.; Croteau, M.-N.

2008-01-01

Determining the exposure of organisms to contaminants is a key component of Ecological Risk Assessments (ERAs). Effective estimates of exposure consider not only the total concentrations of contaminants in an organism's surroundings but also the availability of the contaminants to organisms. Contaminant availability can be inferred from mechanistic models and verified by measurements of contaminant concentrations in organisms. We evaluated the widespread lake-dwelling insect Chaoborus as a potential biomonitor for use in exposure assessments for three metals: cadmium (Cd), copper (Cu), and zinc (Zn). We show that larvae of this midge maintain constant their concentrations of the essential metals Cu and Zn and thus cannot be used to monitor them. In contrast, larval Cd concentrations varied widely both among lakes and in a given lake over time. We were able to relate these variations in biomonitor Cd to changes in lakewater Cd and pH using the Free Ion Activity Model (FIAM). Our results suggest that Chaoborus larvae could be used as an effective tool for estimating the Cd exposure of organisms in lakes for the purposes of ERAs.

Long term plant biomonitoring in the vicinity of waste incinerators in The Netherlands.

PubMed

van Dijk, Chris; van Doorn, Wim; van Alfen, Bert

2015-03-01

Since the mid-nineties new waste incineration plants have come into operation in the Netherlands. Burning of waste can result in the emission of potentially toxic compounds. Although the incineration plants must comply with strict conditions concerning emission control, public concern on the possible impact on human health and the environment still exists. Multiple year (2004-2013) biomonitoring programs were set up around three waste incinerators for early detection of possible effects of stack emissions on the quality of crops and agricultural products. The results showed that the emissions did not affect the quality of crops and cow milk. Concentrations of heavy metals, PAHs and dioxins/PCBs were generally similar to background levels and did not exceed standards for maximum allowable concentrations in foodstuffs (e.g. vegetables and cow milk). Some exceedances of the fluoride standard for cattle feed were found almost every year in the maximum deposition areas of two incinerators. Biomonitoring with leafy vegetables can be used to monitor the real impact of these emissions on agricultural crops and to communicate with all stakeholders. Copyright © 2014 Elsevier Ltd. All rights reserved.

Biologic Monitoring of Exposure to Environmental Chemicals throughout the Life Stages: Requirements and Issues for Consideration for the National Children’s Study

PubMed Central

Barr, Dana B.; Wang, Richard Y.; Needham, Larry L.

2005-01-01

Biomonitoring of exposure is a useful tool for assessing environmental exposures. The matrices available for analyses include blood, urine, breast milk, adipose tissue, and saliva, among others. The sampling can be staged to represent the particular time period of concern: preconceptionally from both parents, from a pregnant woman during each of the three trimesters, during and immediately after childbirth, from the mother postnatally, and from the child as it develops to 21 years of age. The appropriate sample for biomonitoring will depend upon matrix availability, the time period of concern for a particular exposure or health effect, and the different classes of environmental chemicals to be monitored. This article describes the matrices available for biomonitoring during the life stages being evaluated in the National Children’s Study; the best biologic matrices for exposure assessment for each individual chemical class, including consideration of alternative matrices; the analytical methods used for analysis, including quality control procedures and less costly alternatives; the costs of analysis; optimal storage conditions; and chemical and matrix stability during long-term storage. PMID:16079083

Biomonitoring air quality during and after a public transportation strike in the center of Uberlândia, Minas Gerais, Brazil by Tradescantia micronucleus bioassay.

PubMed

Pereira, Boscolli Barbosa; de Campos, Edimar Olegário; de Lima, Euclides Antônio Pereira; Barrozo, Marcos Antonio Souza; Morelli, Sandra

2014-03-01

The aim of this study was to address the lack of information concerning the air quality in the city of Uberlândia, Minas Gerais, Brazil. In this study, we conducted an unprecedented experiment involving the in situ biomonitoring of air genotoxicity in the city center during and after a public transportation strike using the Tradescantia micronucleus test. The frequency of micronuclei was significantly higher in the city center compared with the reference site (Mann-Whitney test, p < 0.05), with the highest MN levels being observed during public transport stoppage (Kruskal-Wallis, Dunn p < 0.01). In addition, the multiple linear regression analyses revealed that the low circulation of buses during public transport stoppage and the increase in the concentration of particulate matter from the increased flow of vehicles in the city center during the strike positively influenced the MN frequency. The climatic factors did not change during the biomonitoring period, reflecting the fact that climatic factors did not influence the MN frequency.

Cytological stress and element uptake in moss and lichen exposed in bags in urban area.

PubMed

Spagnuolo, V; Zampella, M; Giordano, S; Adamo, P

2011-07-01

In this study cytological ultrastructure, total content of C, N and S, and cellular location of major and trace elements (K, Ca, Mg, Cu, Pb and Zn) were investigated in the moss Hypnum cupressiforme and in the lichen Pseudevernia furfuracea exposed in bags for a spring-summer 12-weeks period in the urban area of Naples city. In the moss, severe ultrastructural damages, such as membrane interruptions and dehydration, developed after exposure supporting the occurrence of a dead biomonitor. In the lichen, the post-exposure stress marks, such as the development of lysosome-like vesicles and concentric bodies, or the production of melanin, were overall compatible with life. With exposure, N, S, major and trace element contents all increased in both biomonitors, while C remained substantially unchanged. Copper and Pb were mainly retained in extracellular and particulate forms. Intracellular concentration of Zn consistently increased in both biomonitors, irrespective of their vitality. In transplants, cellular location of elements can better reflect the form in which they occur in the environment. Copyright © 2011 Elsevier Inc. All rights reserved.

Fluid infusion system

NASA Technical Reports Server (NTRS)

1974-01-01

Performance testing carried out in the development of the prototype zero-g fluid infusion system is described and summarized. Engineering tests were performed in the course of development, both on the original breadboard device and on the prototype system. This testing was aimed at establishing baseline system performance parameters and facilitating improvements. Acceptance testing was then performed on the prototype system to verify functional performance. Acceptance testing included a demonstration of the fluid infusion system on a laboratory animal.

Final prototype of magnetically suspended flywheel energy storage system

NASA Technical Reports Server (NTRS)

Anand, D. K.; Kirk, J. A.; Zmood, R. B.; Pang, D.; Lashley, C.

1991-01-01

A prototype of a 500 Wh magnetically suspended flywheel energy storage system was designed, built, and tested. The authors present the work done and include the following: (1) a final design of the magnetic bearing, control system, and motor/generator, (2) construction of a prototype system consisting of the magnetic bearing stack, flywheel, motor, container, and display module, and (3) experimental results for the magnetic bearings, motor, and the entire system. The successful completion of the prototype system has achieved: (1) manufacture of tight tolerance bearings, (2) stability and spin above the first critical frequency, (3) use of inside sensors to eliminate runout problems, and (4) integration of the motor and magnetic bearings.

Final prototype of magnetically suspended flywheel energy storage system

NASA Astrophysics Data System (ADS)

Anand, D. K.; Kirk, J. A.; Zmood, R. B.; Pang, D.; Lashley, C.

A prototype of a 500 Wh magnetically suspended flywheel energy storage system was designed, built, and tested. The authors present the work done and include the following: (1) a final design of the magnetic bearing, control system, and motor/generator, (2) construction of a prototype system consisting of the magnetic bearing stack, flywheel, motor, container, and display module, and (3) experimental results for the magnetic bearings, motor, and the entire system. The successful completion of the prototype system has achieved: (1) manufacture of tight tolerance bearings, (2) stability and spin above the first critical frequency, (3) use of inside sensors to eliminate runout problems, and (4) integration of the motor and magnetic bearings.

PERTS: A Prototyping Environment for Real-Time Systems

NASA Technical Reports Server (NTRS)

Liu, Jane W. S.; Lin, Kwei-Jay; Liu, C. L.

1993-01-01

PERTS is a prototyping environment for real-time systems. It is being built incrementally and will contain basic building blocks of operating systems for time-critical applications, tools, and performance models for the analysis, evaluation and measurement of real-time systems and a simulation/emulation environment. It is designed to support the use and evaluation of new design approaches, experimentations with alternative system building blocks, and the analysis and performance profiling of prototype real-time systems.

Freight Advanced Traveler Information System (FRATIS) – Dallas-Fort Worth (DFW) prototype : final report.

DOT National Transportation Integrated Search

2015-05-01

This is the Final Report for the FRATIS Dallas-Fort Worth DFW prototype system. The FRATIS prototype in DFW consisted of the following components: optimization algorithm, terminal wait time, route specific navigation/traffic/weather, and advanced not...

A Description of a Prototype System at NTID which Merges Computer Assisted Instruction and Instructional Television.

ERIC Educational Resources Information Center

vonFeldt, James R.

The development of a prototype system is described which merges the strengths of computer assisted instruction, data gathering, interactive learning, individualized instruction, and the motion in color, and audio features of television. Creation of the prototype system will allow testing of both TV and interactive CAI/TV strategies in auditory and…

[The waterpipe (shisha) - indoor air quality, human biomonitoring, and health effects].

PubMed

Fromme, Hermann; Schober, Wolfgang

2016-12-01

Waterpipe (WP) smoking, also known as shisha or hookah smoking, is growing in western countries as an alternative to cigarette smoking, especially in younger age groups. A majority of smokers mistakenly believe that shisha smoking is a social entertainment practice that leads to more social behavior and relaxation and that this type of smoking is safe or less harmful and less addictive than cigarette smoking.In reality, WP smokers are exposed to hundreds of toxic substances that include well-known carcinogens. High exposures to carbon monoxide and nicotine are major health threats. There is growing evidence that WP smoke causes adverse effects on the pulmonary and cardiovascular systems and there are indications that WP smoke is associated with cancer. Persons exposed to secondhand WP smoke are also at risk.More research on the health effects of WP is urgently needed and more preventive measures for public health protection. Moreover, public WP facilities should be implemented under specific nonsmoker protection laws and consequently controlled.This review summarizes recent data on exposure to WP smoking in indoor environments, the results of biomonitoring data, and the known health effects based on currently available toxicological or epidemiological studies.

Trace element biomonitoring using mosses in urban areas affected by mud volcanoes around Mt. Etna. The case of the Salinelle, Italy.

PubMed

Bonanno, Giuseppe; Lo Giudice, Rosa; Pavone, Pietro

2012-08-01

Trace element impact was assessed using mosses in a densely inhabited area affected by mud volcanoes. Such volcanoes, locally called Salinelle, are phenomena that occur around Mt. Etna (Sicily, Italy) and are interpreted as the surface outflow of a hydrothermal system located below Mt. Etna, releasing sedimentary fluids (hydrocarbons and NaCl brines) along with magmatic gases (mainly CO(2) and He). To date, scarce data are available about the presence of trace elements, and no biomonitoring campaigns are reported about the cumulative effects of such emissions. In this study, concentrations of Al, As, Cd, Cr, Cu, Hg, Mn, Ni, Pb, V, and Zn were detected in the moss Bryum argenteum, in soil and water. Results showed that the trace element contribution of the Salinelle to the general pollution was significant for Al, Mn, Ni, and Zn. The comparison of trace concentrations in mosses from Salinelle and Etna showed that the mud volcanoes release a greater amount of Al and Mn, whereas similar values of Ni were found. Natural emissions of trace elements could be hazardous in human settlements, in particular, the Salinelle seem to play an important role in environmental pollution.

Spacelab data processing facility (SLDPF) quality assurance (QA)/data accounting (DA) expert systems - Transition from prototypes to operational systems

NASA Technical Reports Server (NTRS)

Basile, Lisa

1988-01-01

The SLDPF is responsible for the capture, quality monitoring processing, accounting, and shipment of Spacelab and/or Attached Shuttle Payloads (ASP) telemetry data to various user facilities. Expert systems will aid in the performance of the quality assurance and data accounting functions of the two SLDPF functional elements: the Spacelab Input Processing System (SIPS) and the Spacelab Output Processing System (SOPS). Prototypes were developed for each as independent efforts. The SIPS Knowledge System Prototype (KSP) used the commercial shell OPS5+ on an IBM PC/AT; the SOPS Expert System Prototype used the expert system shell CLIPS implemented on a Macintosh personal computer. Both prototypes emulate the duties of the respective QA/DA analysts based upon analyst input and predetermined mission criteria parameters, and recommended instructions and decisions governing the reprocessing, release, or holding for further analysis of data. These prototypes demonstrated feasibility and high potential for operational systems. Increase in productivity, decrease of tedium, consistency, concise historical records, and a training tool for new analyses were the principal advantages. An operational configuration, taking advantage of the SLDPF network capabilities, is under development with the expert systems being installed on SUN workstations. This new configuration in conjunction with the potential of the expert systems will enhance the efficiency, in both time and quality, of the SLDPF's release of Spacelab/AST data products.

Spacelab data processing facility (SLDPF) Quality Assurance (QA)/Data Accounting (DA) expert systems: Transition from prototypes to operational systems

NASA Technical Reports Server (NTRS)

Basile, Lisa

1988-01-01

The SLDPF is responsible for the capture, quality monitoring processing, accounting, and shipment of Spacelab and/or Attached Shuttle Payloads (ASP) telemetry data to various user facilities. Expert systems will aid in the performance of the quality assurance and data accounting functions of the two SLDPF functional elements: the Spacelab Input Processing System (SIPS) and the Spacelab Output Processing System (SOPS). Prototypes were developed for each as independent efforts. The SIPS Knowledge System Prototype (KSP) used the commercial shell OPS5+ on an IBM PC/AT; the SOPS Expert System Prototype used the expert system shell CLIPS implemented on a Macintosh personal computer. Both prototypes emulate the duties of the respective QA/DA analysts based upon analyst input and predetermined mission criteria parameters, and recommended instructions and decisions governing the reprocessing, release, or holding for further analysis of data. These prototypes demonstrated feasibility and high potential for operational systems. Increase in productivity, decrease of tedium, consistency, concise historial records, and a training tool for new analyses were the principal advantages. An operational configuration, taking advantage of the SLDPF network capabilities, is under development with the expert systems being installed on SUN workstations. This new configuration in conjunction with the potential of the expert systems will enhance the efficiency, in both time and quality, of the SLDPF's release of Spacelab/AST data products.

46 CFR 160.135-11 - Fabrication of prototype lifeboats for approval.

Code of Federal Regulations, 2013 CFR

2013-10-01

.... The Commandant may prescribe additional prototype tests and inspections necessary to maintain quality... inspecting— (i) Fiber Reinforced Plastic (FRP) Construction. (A) FRP components of each prototype lifeboat... system; (vii) Installation of the steering system; and (viii) Installation of the water spray fire...

46 CFR 160.135-11 - Fabrication of prototype lifeboats for approval.

Code of Federal Regulations, 2012 CFR

2012-10-01

.... The Commandant may prescribe additional prototype tests and inspections necessary to maintain quality... inspecting— (i) Fiber Reinforced Plastic (FRP) Construction. (A) FRP components of each prototype lifeboat... system; (vii) Installation of the steering system; and (viii) Installation of the water spray fire...

46 CFR 160.135-11 - Fabrication of prototype lifeboats for approval.

Code of Federal Regulations, 2014 CFR

2014-10-01

.... The Commandant may prescribe additional prototype tests and inspections necessary to maintain quality... inspecting— (i) Fiber Reinforced Plastic (FRP) Construction. (A) FRP components of each prototype lifeboat... system; (vii) Installation of the steering system; and (viii) Installation of the water spray fire...

A review method for UML requirements analysis model employing system-side prototyping.

PubMed

Ogata, Shinpei; Matsuura, Saeko

2013-12-01

User interface prototyping is an effective method for users to validate the requirements defined by analysts at an early stage of a software development. However, a user interface prototype system offers weak support for the analysts to verify the consistency of the specifications about internal aspects of a system such as business logic. As the result, the inconsistency causes a lot of rework costs because the inconsistency often makes the developers impossible to actualize the system based on the specifications. For verifying such consistency, functional prototyping is an effective method for the analysts, but it needs a lot of costs and more detailed specifications. In this paper, we propose a review method so that analysts can verify the consistency among several different kinds of diagrams in UML efficiently by employing system-side prototyping without the detailed model. The system-side prototype system does not have any functions to achieve business logic, but visualizes the results of the integration among the diagrams in UML as Web pages. The usefulness of our proposal was evaluated by applying our proposal into a development of Library Management System (LMS) for a laboratory. This development was conducted by a group. As the result, our proposal was useful for discovering the serious inconsistency caused by the misunderstanding among the members of the group.

SIMS prototype system 1 test results: Engineering analysis

NASA Technical Reports Server (NTRS)

1978-01-01

The space and domestic water solar heating system designated SIMS Prototype Systems 1 was evaluated. The test system used 720 ft (gross) of Solar Energy Products Air Collectors, a Solar Control Corporation SAM 20 Air Handler with Model 75-175 control unit, a Jackson Solar Storage tank with Rho Sigma Mod 106 controller, and 20 tons of rack storage. The test data analysis performed evaluates the system performance and documents the suitability of SIMS Prototype System 1 hardware for field installation.

Integration of the Execution Support System for the Computer-Aided Prototyping System (CAPS)

DTIC Science & Technology

1990-09-01

SUPPORT SYSTEM FOR THE COMPUTER -AIDED PROTOTYPING SYSTEM (CAPS) by Frank V. Palazzo September 1990 Thesis Advisor: Luq± Approved for public release...ZATON REPOR ,,.VBE (, 6a NAME OF PERPORMING ORGAN ZAT7ON 6b OFF:CE SYVBOL 7a NAME OF MONITORINC O0-CA’Za- ON Computer Science Department (if applicable...Include Security Classification) Integration of the Execution Support System for the Computer -Aided Prototyping System (C S) 12 PERSONAL AUTHOR(S) Frank V

Efficient Computational Prototyping of Mixed Technology Microfluidic Components and Systems

DTIC Science & Technology

2002-08-01

AFRL-IF-RS-TR-2002-190 Final Technical Report August 2002 EFFICIENT COMPUTATIONAL PROTOTYPING OF MIXED TECHNOLOGY MICROFLUIDIC...SUBTITLE EFFICIENT COMPUTATIONAL PROTOTYPING OF MIXED TECHNOLOGY MICROFLUIDIC COMPONENTS AND SYSTEMS 6. AUTHOR(S) Narayan R. Aluru, Jacob White...Aided Design (CAD) tools for microfluidic components and systems were developed in this effort. Innovative numerical methods and algorithms for mixed

Supporting Active Patient and Health Care Collaboration: A Prototype for Future Health Care Information Systems.

PubMed

Åhlfeldt, Rose-Mharie; Persson, Anne; Rexhepi, Hanife; Wåhlander, Kalle

2016-12-01

This article presents and illustrates the main features of a proposed process-oriented approach for patient information distribution in future health care information systems, by using a prototype of a process support system. The development of the prototype was based on the Visuera method, which includes five defined steps. The results indicate that a visualized prototype is a suitable tool for illustrating both the opportunities and constraints of future ideas and solutions in e-Health. The main challenges for developing and implementing a fully functional process support system concern both technical and organizational/management aspects. © The Author(s) 2015.

Development of Intelligent Spray Systems for Nursery Crop Production

USDA-ARS?s Scientific Manuscript database

Two intelligent sprayer prototypes were developed to increase pesticide application efficiency in nursery production. The first prototype was a hydraulic vertical boom system using ultrasonic sensors to detect tree size and volume for liner-sized trees and the second prototype was an air-assisted sp...

Silver vanadate nanoribbons: A label-free bioindicator in the conversion between human serum transferrin and apotransferrin via surface-enhanced Raman scattering

NASA Astrophysics Data System (ADS)

Zhou, Qing; Shao, Mingwang; Que, Ronghui; Cheng, Liang; Zhuo, Shujuan; Tong, Yanhua; Lee, Shuit-Tong

2011-05-01

Silver vanadate nanoribbons were synthesized via a hydrothermal process, which exhibited surface-enhanced Raman scattering effect. This surface-enhanced substrate was stable and reproducible for identifying human serum transferrin and human serum apotransferrin in the concentration of 1×10-5 M, which further exhibited significant sensitivity in monitoring the conversion of these two proteins in turn. This result showed that the silver vanadate nanoribbon might be employed as biomonitor in such systems.

Feasibility of Biomonitoring of Exposure to Permethrin Through Analysis of Long-Lived (Metabolite) Adducts to Proteins

DTIC Science & Technology

2009-04-01

BaCO3 N2 koeler Ba(OH)2 opl. ventiel Figure 2. Manifold for preparation of [14C] 3-PBA. Preparation of the Grignard reagent To flask E...The entire system was kept under nitrogen. The preparation of the Grignard reagent was initiated by applying heat using a heat gun. When the reaction...all reagents were present in the manifold prior to the beginning of the experiment. 24 A B E D C drukmetervacuumH2SO4

Feasibility of Biomonitoring of Exposure to Permethrin Through Analysis of Long-Lived (Metabolite) Adducts to Proteins

DTIC Science & Technology

2007-09-01

Preparation of the Grignard reagent To flask E, containing magnesium (52 mg, 2 mmol) and a few crystals of iodine was added a solution of 3...bromodiphenylether (750 mg, 3 mmol) in diethyl ether (3 mL). The entire system was kept under nitrogen. The preparation of the Grignard reagent was initiated by...device represented below was used for the multi-step synthesis of 3-PBA; all reagents were present in the manifold prior to the beginning of the

Highly Sensitive and Selective Immuno-capture/Electrochemical Assay of Acetylcholinesterase Activity in Red Blood Cells: A Biomarker of Exposure to Organophosphorus Pesticides and Nerve Agents

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Aiqiong; Du, Dan; Lin, Yuehe

Acetylcholinesterase (AChE) enzyme activity in red blood cells (RBCs) is a useful biomarker for biomonitoring of exposures to organophosphorus (OP) pesticides and chemical nerve agents. In this paper, we reported a new method for AChE activity assay based on selective immuno-capture of AChE from biological samples followed by enzyme activity assay of captured AChE using a disposable electrochemical sensor. The electrochemical sensor is based on multiwalled carbon nanotubes-gold nanocomposites (MWCNTs-Au) modified screen printed carbon electrode (SPCE). Upon the completion of immunoreaction, the target AChE (including active and inhibited) is captured onto the electrode surface and followed by an electrochemical detectionmore » of enzymatic activity in the presence of acetylthiocholine. A linear response is obtained over standard AChE concentration range from 0.1 to 10 nM. To demonstrate the capability of this new biomonitoring method, AChE solutions dosed with different concentration of paraoxon were used to validate the new AChE assay method. AChE inhibition in OP dosed solutions was proportional to its concentration from 0.2 to 50 nM. The new AChE activity assay method for biomonitoring of OP exposure was further validated with in-vitro paraoxon-dosed RBC samples. The established electrochemical sensing platform for AChE activity assay not only avoids the problem of overlapping substrate specificity with esterases by using selective antibody, but also eliminates potential interference from other electroactive species in biological samples. It offers a new approach for sensitive, selective, and rapid AChE activity assay for biomonitoring of exposures to OPs.« less

On line biomonitors used as a tool for toxicity reduction evaluation of in situ groundwater remediation techniques.

PubMed

Küster, Eberhard; Dorusch, Falk; Vogt, Carsten; Weiss, Holger; Altenburger, Rolf

2004-07-15

Success of groundwater remediation is typically controlled via snapshot analysis of selected chemical substances or physical parameters. Biological parameters, i.e. ecotoxicological assays, are rarely employed. Hence the aim of the study was to develop a bioassay tool, which allows an on line monitoring of contaminated groundwater, as well as a toxicity reduction evaluation (TRE) of different remediation techniques in parallel and may furthermore be used as an additional tool for process control to supervise remediation techniques in a real time mode. Parallel testing of groundwater remediation techniques was accomplished for short and long time periods, by using the energy dependent luminescence of the bacterium Vibrio fischeri as biological monitoring parameter. One data point every hour for each remediation technique was generated by an automated biomonitor. The bacteria proved to be highly sensitive to the contaminated groundwater and the biomonitor showed a long standing time despite the highly corrosive groundwater present in Bitterfeld, Germany. The bacterial biomonitor is demonstrated to be a valuable tool for remediation success evaluation. Dose response relationships were generated for the six quantitatively dominant groundwater contaminants (2-chlortoluene, 1,2- and 1,4-dichlorobenzene, monochlorobenzene, ethylenbenzene and benzene). The concentrations of individual volatile organic chemicals (VOCs) could not explain the observed effects in the bacteria. An expected mixture toxicity was calculated for the six components using the concept of concentration addition. The calculated EC(50) for the mixture was still one order of magnitude lower than the observed EC(50) of the actual groundwater. The results pointed out that chemical analysis of the six most quantitative substances alone was not able to explain the effects observed with the bacteria. Thus chemical analysis alone may not be an adequate tool for remediation success evaluation in terms of toxicity reduction.

Development and application of LC–APCI–MS method for biomonitoring of animal and human exposure to imidacloprid.

PubMed

Kavvalakis, Matthaios P; Tzatzarakis, Manolis N; Theodoropoulou, Eleftheria P; Barbounis, Emmanouil G; Tsakalof, Andreas K; Tsatsakis, Aristidis M

2013-11-01

Imidacloprid (IMI) is a relatively new neuro-active neonicotinoid insecticide and nowadays one of the largest selling insecticides worldwide. In the present study a LC–APCI–MS based method was developed and validated for the quantification of imidacloprid and its main metabolite 6-chloronicotinic acid (6- CINA) in urine and hair specimens. The method was tested in biomonitoring of intentionally exposed animals and subsequently applied for biomonitoring of Cretan urban and rural population. The developed analytical method comprises two main steps of analytes isolation from specimen (solid– liquid extraction with methanol for hair, liquid–liquid extraction with methanol for urine) and subsequent instrumental analysis by LC–APCI–MS. The developed method was applied for the monitoring of IMI and 6-ClNA in hair and urine of laboratory animals (rabbits) intentionally fed with insecticide at low or high doses (40 and 80 mg kg(-1) weight d(-1) respectively) for 24 weeks. The analytes were detected in the regularly acquired hair and urine specimens and their found levels were proportional to the feeding dose and time of exposure with the exception of slight decline of IMI levels in high dose fed rabbits after 24 weeks of feeding. This decline can be explained by the induction of IMI metabolizing enzymes by the substrate. After testing on animal models the method was applied for pilot biomonitoring of Crete urban (n = 26) and rural (n = 32) population. Rural but not urban population is exposed to IMI with 21 positive samples (65.6%) and found median concentration 0.03 ng mg(-1). Maximum concentration detected was 27 ng mg(-1)

Persistent organic pollutants in atmospheric deposition and biomonitoring with Tillandsia Usneoides (L.) in an industrialized area in Rio de Janeiro state, south east Brazil--Part I: PCDD and PCDF.

PubMed

de Souza Pereira, Márcia; Waller, Ulrich; Reifenhäuser, Werner; Torres, João Paulo M; Malm, Olaf; Körner, Wolfgang

2007-04-01

Monitoring of immission of persistent organic pollutants in the industrialized area of Volta Redonda (V.R.) and in the National Park of Itatiaia (PNI) in southeast Brazil was performed using an endemic Bromeliad species as biomonitor and measuring total deposition rates on funnels covered with polyurethane foams. Samples were collected during 78 days in V.R. and 95 days in PNI in winter (dry season, June-August 2003) and during 114 days in both areas in summer (rainy season, December 2003-February 2004). The PCDD/PCDF deposition rates ranged from 0.10 to 1.9 pg WHO-TEQ/(m2 day) in winter and from 0.11 to 2.2 pg WHO-TEQ/(m2 day) in summer. Deposition rates found in V.R. in summer were four- to ninefold higher than those measured in PNI, while in winter deposition rates in both regions were in the same range. Deposition rates in V.R. in summer were about five fold lower than those measured in 1996. PCDD/PCDF levels in biomonitor samples were between 0.95 and 14.6 ng WHO-TEQ/kg d.m. in winter and between 2.2 and 5.2 ng WHO-TEQ/kg d.m. in summer. In winter, concentrations found in V.R. were up to 11 times higher than those found in PNI, while in summer the levels measured in both areas were comparable. The homologue and isomer profiles found in the deposition as well as in the biomonitor samples from V.R. indicate that steel production is the main source of contamination in the region, whereas in PNI, the long range transport of these pollutants is the predominant contamination pathway.

Human biomonitoring reference values for some non-persistent chemicals in blood and urine derived from the Canadian Health Measures Survey 2009-2013.

PubMed

Khoury, Cheryl; Werry, Kate; Haines, Douglas; Walker, Mike; Malowany, Morie

2018-05-01

The Canadian Health Measures Survey collects nationally representative human biomonitoring data on a suite of chemicals and their metabolites, including many non-persistent chemicals. Data has been collected on non-persistent chemicals, including acrylamide, chlorophenols, environmental phenols and triclocarban, organophosphate insecticides, phthalates, polycyclic aromatic hydrocarbon, pyrethroid insecticides, and volatile organic compounds from 2009 to 2013. Using a systematic approach building on the reference interval concept proposed by the International Federation of Clinical Chemistry and Laboratory Medicine and the International Union of Pure and Applied Chemistry, we derive human biomonitoring reference values (RV 95 s) for these classes of non-persistent chemicals in blood and urine for the general Canadian population. RV 95 s were derived for biomarkers of non-persistent chemicals with widespread detection in Canadians (>66% detection rate). Samples with urinary creatinine levels outside the recommended range of 0.3-3.0 μg/L were excluded. Reference populations were constructed by applying smoking and fasting as exclusion criteria where appropriate. Age and sex were evaluated as possible partitioning criteria and separate RV 95 s were derived for sub-populations in cases where partitioning was deemed necessary. Reference values were derived for 40 biomarkers and represent the first set of RV 95 s for non-persistent chemicals in the general Canadian population. These values provide a measure of the upper margin of background exposure in the general population and can be compared against individual and population human biomonitoring data. RV 95 s can be used to by public health officials to identify individuals with high exposures, and by risk assessors and risk managers to identify atypical exposures or subpopulations with elevated exposures. Crown Copyright © 2018. Published by Elsevier GmbH. All rights reserved.

Improved detection of coastal acid sulfate soil hotspots through biomonitoring of metal(loid) accumulation in water lilies (Nymphaea capensis).

PubMed

Stroud, Jacqueline L; Collins, Richard N

2014-07-15

Anthropogenically disturbed coastal acid sulfate soils along the east coast of Australia, and worldwide, periodically result in the discharge of acid waters containing high concentrations of metals. Identifying priority sites (hotspots) within a catchment for acid sulfate soil remediation activities typically involves long-term monitoring of drainwater chemistry, including the capture of data on unpredictable rain-induced groundwater discharge events. To improve upon this monitoring approach, this study investigated using the water lily (Nymphaea capensis) as a biomonitor of drainage waters to identify hotspots in three acid sulfate soil impacted catchments (83 km(2)) in north-eastern New South Wales, Australia. In one catchment where the location of hotspots was known, water lily lamina concentrations of a suite of metal(loid)s were significantly (p<0.05) higher than plants collected from an unpolluted 'reference' drainage channel, thus validating the concept of using this species as a biomonitor. A catchment-scale water lily sampling program undertaken in catchments with unidentified hotspots revealed within catchment variation of plant metal concentrations up to 70-fold. High resolution maps produced from these results, therefore, provided strong evidence for the location of potential hotspots which were confirmed with measurements of drainwater chemistry during rain-induced groundwater discharge events. Median catchment lily accumulation was ca. 160 mg Al kg(-1) and 1,300 mg Fe kg(-1), with hotspots containing up to 6- and 10-fold higher Al and Fe concentrations. These findings suggest that biomonitoring with N. capensis can be an important tool to rapidly identify priority sites for remediation in acid sulfate soil impacted landscapes. Copyright © 2014 Elsevier B.V. All rights reserved.

A multi-element screening method to identify metal targets for blood biomonitoring in green sea turtles (Chelonia mydas).

PubMed

Villa, C A; Finlayson, S; Limpus, C; Gaus, C

2015-04-15

Biomonitoring of blood is commonly used to identify and quantify occupational or environmental exposure to chemical contaminants. Increasingly, this technique has been applied to wildlife contaminant monitoring, including for green turtles, allowing for the non-lethal evaluation of chemical exposure in their nearshore environment. The sources, composition, bioavailability and toxicity of metals in the marine environment are, however, often unknown and influenced by numerous biotic and abiotic factors. These factors can vary considerably across time and space making the selection of the most informative elements for biomonitoring challenging. This study aimed to validate an ICP-MS multi-element screening method for green turtle blood in order to identify and facilitate prioritisation of target metals for subsequent fully quantitative analysis. Multi-element screening provided semiquantitative results for 70 elements, 28 of which were also determined through fully quantitative analysis. Of the 28 comparable elements, 23 of the semiquantitative results had an accuracy between 67% and 112% relative to the fully quantified values. In lieu of any available turtle certified reference materials (CRMs), we evaluated the use of human blood CRMs as a matrix surrogate for quality control, and compared two commonly used sample preparation methods for matrix related effects. The results demonstrate that human blood provides an appropriate matrix for use as a quality control material in the fully quantitative analysis of metals in turtle blood. An example for the application of this screening method is provided by comparing screening results from blood of green turtles foraging in an urban and rural region in Queensland, Australia. Potential targets for future metal biomonitoring in these regions were identified by this approach. Copyright © 2014 Elsevier B.V. All rights reserved.

The skin of Commerson's dolphins (Cephalorhynchus commersonii) as a biomonitor of mercury and selenium in Subantarctic waters.

PubMed

Cáceres-Saez, Iris; Goodall, R Natalie P; Dellabianca, Natalia A; Cappozzo, H Luis; Ribeiro Guevara, Sergio

2015-11-01

The skin of bycaught Commerson's dolphins was tested for mercury (Hg) and selenium (Se) biomonitoring in Subantarctic environments. The correlation of levels detected in the skin with those found in internal tissues - lung, liver, kidney and muscle - was assessed to evaluate how skin represents internal Hg and Se distribution for monitoring purposes. Mercury in skin had a concentration range of 0.68-3.11 μg g(-1) dry weight (DW), while Se had a higher concentration range of 74.3-124.5 μg g(-1) DW. There was no significant correlation between selenium levels in any of the analyzed tissues. Thus, the skin selenium concentration did not reflect the tissular Se levels and did not provide information for biomonitoring. The lack of correlation is explained by the biological role of Se, provided that each tissue regulates Se levels according to physiological needs. However, the skin Hg level had significant positive correlation with the levels in internal tissues (ANOVA p<0.05), particularly with that of muscle (R(2)=0.79; ANOVA p=0.0008). Thus, this correlation permits the estimation of Hg content in muscle based on the multiplication of skin biopsy levels by a factor of 1.85. Mercury bioindication using skin biopsies is a non-lethal approach that allows screening of a large number of specimens with little disturbance and makes possible an adequate sampling strategy that produces statistically valid results in populations and study areas. The correlation between Hg levels in the skin and internal tissues supports the use of the epidermis of Commerson's dolphins for Hg biomonitoring in the waters of the Subantarctic, which is a poorly studied region regarding Hg levels, sources and processes. Copyright © 2015 Elsevier Ltd. All rights reserved.

Evaluating ethanol-based sample preservation to facilitate use of DNA barcoding in routine freshwater biomonitoring programs using benthic macroinvertebrates.

PubMed

Stein, Eric D; White, Bryan P; Mazor, Raphael D; Miller, Peter E; Pilgrim, Erik M

2013-01-01

Molecular methods, such as DNA barcoding, have the potential to enhance biomonitoring programs worldwide. Altering routinely used sample preservation methods to protect DNA from degradation may pose a potential impediment to application of DNA barcoding and metagenomics for biomonitoring using benthic macroinvertebrates. Using higher volumes or concentrations of ethanol, requirements for shorter holding times, or the need to include additional filtering may increase cost and logistical constraints to existing biomonitoring programs. To address this issue we evaluated the efficacy of various ethanol-based sample preservation methods at maintaining DNA integrity. We evaluated a series of methods that were minimally modified from typical field protocols in order to identify an approach that can be readily incorporated into existing monitoring programs. Benthic macroinvertebrates were collected from a minimally disturbed stream in southern California, USA and subjected to one of six preservation treatments. Ten individuals from five taxa were selected from each treatment and processed to produce DNA barcodes from the mitochondrial gene cytochrome c oxidase I (COI). On average, we obtained successful COI sequences (i.e. either full or partial barcodes) for between 93-99% of all specimens across all six treatments. As long as samples were initially preserved in 95% ethanol, successful sequencing of COI barcodes was not affected by a low dilution ratio of 2∶1, transfer to 70% ethanol, presence of abundant organic matter, or holding times of up to six months. Barcoding success varied by taxa, with Leptohyphidae (Ephemeroptera) producing the lowest barcode success rate, most likely due to poor PCR primer efficiency. Differential barcoding success rates have the potential to introduce spurious results. However, routine preservation methods can largely be used without adverse effects on DNA integrity.

Evaluating Ethanol-based Sample Preservation to Facilitate Use of DNA Barcoding in Routine Freshwater Biomonitoring Programs Using Benthic Macroinvertebrates

PubMed Central

Stein, Eric D.; White, Bryan P.; Mazor, Raphael D.; Miller, Peter E.; Pilgrim, Erik M.

2013-01-01

Molecular methods, such as DNA barcoding, have the potential to enhance biomonitoring programs worldwide. Altering routinely used sample preservation methods to protect DNA from degradation may pose a potential impediment to application of DNA barcoding and metagenomics for biomonitoring using benthic macroinvertebrates. Using higher volumes or concentrations of ethanol, requirements for shorter holding times, or the need to include additional filtering may increase cost and logistical constraints to existing biomonitoring programs. To address this issue we evaluated the efficacy of various ethanol-based sample preservation methods at maintaining DNA integrity. We evaluated a series of methods that were minimally modified from typical field protocols in order to identify an approach that can be readily incorporated into existing monitoring programs. Benthic macroinvertebrates were collected from a minimally disturbed stream in southern California, USA and subjected to one of six preservation treatments. Ten individuals from five taxa were selected from each treatment and processed to produce DNA barcodes from the mitochondrial gene cytochrome c oxidase I (COI). On average, we obtained successful COI sequences (i.e. either full or partial barcodes) for between 93–99% of all specimens across all six treatments. As long as samples were initially preserved in 95% ethanol, successful sequencing of COI barcodes was not affected by a low dilution ratio of 2∶1, transfer to 70% ethanol, presence of abundant organic matter, or holding times of up to six months. Barcoding success varied by taxa, with Leptohyphidae (Ephemeroptera) producing the lowest barcode success rate, most likely due to poor PCR primer efficiency. Differential barcoding success rates have the potential to introduce spurious results. However, routine preservation methods can largely be used without adverse effects on DNA integrity. PMID:23308097

Integrated prototyping environment for programmable automation

NASA Astrophysics Data System (ADS)

da Costa, Francis; Hwang, Vincent S. S.; Khosla, Pradeep K.; Lumia, Ronald

1992-11-01

We propose a rapid prototyping environment for robotic systems, based on tenets of modularity, reconfigurability and extendibility that may help build robot systems `faster, better, and cheaper.' Given a task specification, (e.g., repair brake assembly), the user browses through a library of building blocks that include both hardware and software components. Software advisors or critics recommend how blocks may be `snapped' together to speedily construct alternative ways to satisfy task requirements. Mechanisms to allow `swapping' competing modules for comparative test and evaluation studies are also included in the prototyping environment. After some iterations, a stable configuration or `wiring diagram' emerges. This customized version of the general prototyping environment still contains all the hooks needed to incorporate future improvements in component technologies and to obviate unplanned obsolescence. The prototyping environment so described is relevant for both interactive robot programming (telerobotics) and iterative robot system development (prototyping).

Prototype Systems for Measuring Outdoor Air Intake Rates in Rooftop Air Handlers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fisk, William J.; Chan, Wanyu R.; Hotchi, Toshifumi

2015-01-01

The widespread absence of systems for real-time measurement and feedback control, of minimum outdoor air intake rates in HVAC systems contributes to the poor control of ventilation rates in commercial buildings. Ventilation rates affect building energy consumption and influence occupant health. The project designed fabricated and tested four prototypes of systems for measuring rates of outdoor air intake into roof top air handlers. All prototypes met the ±20% accuracy target at low wind speeds, with all prototypes accurate within approximately ±10% after application of calibration equations. One prototype met the accuracy target without a calibration. With two of four prototypemore » measurement systems, there was no evidence that wind speed or direction affected accuracy; however, winds speeds were generally below usually 3.5 m s -1 (12.6 km h -1) and further testing is desirable. The airflow resistance of the prototypes was generally less than 35 Pa at maximum RTU air flow rates. A pressure drop of this magnitude will increase fan energy consumption by approximately 4%. The project did not have resources necessary to estimate costs of mass produced systems. The retail cost of components and materials used to construct prototypes ranged from approximately $1,200 to $1,700. The test data indicate that the basic designs developed in this project, particularly the designs of two of the prototypes, have considerable merit. Further design refinement, testing, and cost analysis would be necessary to fully assess commercial potential. The designs and test results will be communicated to the HVAC manufacturing community.« less

Human Factors and Technical Considerations for a Computerized Operator Support System Prototype

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ulrich, Thomas Anthony; Lew, Roger Thomas; Medema, Heather Dawne

2015-09-01

A prototype computerized operator support system (COSS) has been developed in order to demonstrate the concept and provide a test bed for further research. The prototype is based on four underlying elements consisting of a digital alarm system, computer-based procedures, PI&D system representations, and a recommender module for mitigation actions. At this point, the prototype simulates an interface to a sensor validation module and a fault diagnosis module. These two modules will be fully integrated in the next version of the prototype. The initial version of the prototype is now operational at the Idaho National Laboratory using the U.S. Departmentmore » of Energy’s Light Water Reactor Sustainability (LWRS) Human Systems Simulation Laboratory (HSSL). The HSSL is a full-scope, full-scale glass top simulator capable of simulating existing and future nuclear power plant main control rooms. The COSS is interfaced to the Generic Pressurized Water Reactor (gPWR) simulator with industry-typical control board layouts. The glass top panels display realistic images of the control boards that can be operated by touch gestures. A section of the simulated control board was dedicated to the COSS human-system interface (HSI), which resulted in a seamless integration of the COSS into the normal control room environment. A COSS demonstration scenario has been developed for the prototype involving the Chemical & Volume Control System (CVCS) of the PWR simulator. It involves a primary coolant leak outside of containment that would require tripping the reactor if not mitigated in a very short timeframe. The COSS prototype presents a series of operator screens that provide the needed information and soft controls to successfully mitigate the event.« less

Real-time application of knowledge-based systems

NASA Technical Reports Server (NTRS)

Brumbaugh, Randal W.; Duke, Eugene L.

1989-01-01

The Rapid Prototyping Facility (RPF) was developed to meet a need for a facility which allows flight systems concepts to be prototyped in a manner which allows for real-time flight test experience with a prototype system. This need was focused during the development and demonstration of the expert system flight status monitor (ESFSM). The ESFSM was a prototype system developed on a LISP machine, but lack of a method for progressive testing and problem identification led to an impractical system. The RPF concept was developed, and the ATMS designed to exercise its capabilities. The ATMS Phase 1 demonstration provided a practical vehicle for testing the RPF, as well as a useful tool. ATMS Phase 2 development continues. A dedicated F-18 is expected to be assigned for facility use in late 1988, with RAV modifications. A knowledge-based autopilot is being developed using the RPF. This is a system which provides elementary autopilot functions and is intended as a vehicle for testing expert system verification and validation methods. An expert system propulsion monitor is being prototyped. This system provides real-time assistance to an engineer monitoring a propulsion system during a flight.

A knowledge-based system for monitoring the electrical power system of the Hubble Space Telescope

NASA Technical Reports Server (NTRS)

Eddy, Pat

1987-01-01

The design and the prototype for the expert system for the Hubble Space Telescope's electrical power system are discussed. This prototype demonstrated the capability to use real time data from a 32k telemetry stream and to perform operational health and safety status monitoring, detect trends such as battery degradation, and detect anomalies such as solar array failures. This prototype, along with the pointing control system and data management system expert systems, forms the initial Telemetry Analysis for Lockheed Operated Spacecraft (TALOS) capability.

Prototype solar heating and cooling systems

NASA Technical Reports Server (NTRS)

1978-01-01

Eight prototype systems were developed. The systems are 3, 25, and 75-ton size units. The manufacture, test, installation, maintenance, problem resolution, and performance evaluation of the systems is described. Size activities for the various systems are included.

Magnetic Electrochemical Sensing Platform for Biomonitoring of Exposure to Organophosphorus Pesticides and Nerve Agents Based on Simultaneous Measurement of Total Enzyme Amount and Enzyme Activity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Du, Dan; Wang, Jun; Wang, Limin

We report a new approach for electrochemical quantification of enzymatic inhibition and phosphorylation for biomonitoring of exposure to organophosphorus (OP) pesticides and nerve agents based on a magnetic beads (MBs) immunosensing platform. The principle of this approach is based on the combination of MBs immuno-capture based enzyme activity assay and competitive immunoassay of total amount of enzyme for simultaneous detection of enzyme inhibition and phosphorylation in biological fluids. Butyrylcholinesterase (BChE) was chosen as a model enzyme. In competitive immunoassay, the target total BChE in a sample (mixture of OP-inhibited BChE and active BChE) competes with the BChE modified on themore » MBs to bind to the limited anti-BChE antibody labeled with quantum dots (QDs-anti-BChE), and followed by electrochemical stripping analysis of the bound QDs conjugate on the MBs. This assay shows a linear response over the total BChE concentration range of 0.1~20 nM. Simultaneously, real time BChE activity was measured on an electrochemical carbon nanotube-based sensor coupled with microflow injection system after immuno-capture by MBs-anti-BChE conjugate. Therefore, the formed phosphorylated adduct (OP-BChE) can be estimated by the difference values of the total amount BChE (including active and OP-inhibited) and active BChE from established calibration curves. This approach not only eliminates the difficulty in screening of low-dose OP exposure (less than 20% inhibition of BChE) because of individual variation of BChE values, but also avoids the drawback of the scarce availability of OP-BChE antibody. It is sensitive enough to detect 0.5 nM OP-BChE, which is less than 2% BChE inhibition. This method offers a new method for rapid, accurate, selective and inexpensive quantification of phosphorylated adducts and enzyme inhibition for biomonitoring of OP and nerve agent exposures.« less

Operations management system advanced automation: Fault detection isolation and recovery prototyping

NASA Technical Reports Server (NTRS)

Hanson, Matt

1990-01-01

The purpose of this project is to address the global fault detection, isolation and recovery (FDIR) requirements for Operation's Management System (OMS) automation within the Space Station Freedom program. This shall be accomplished by developing a selected FDIR prototype for the Space Station Freedom distributed processing systems. The prototype shall be based on advanced automation methodologies in addition to traditional software methods to meet the requirements for automation. A secondary objective is to expand the scope of the prototyping to encompass multiple aspects of station-wide fault management (SWFM) as discussed in OMS requirements documentation.

Analytical methods for human biomonitoring of pesticides. A review.

PubMed

Yusa, Vicent; Millet, Maurice; Coscolla, Clara; Roca, Marta

2015-09-03

Biomonitoring of both currently-used and banned-persistent pesticides is a very useful tool for assessing human exposure to these chemicals. In this review, we present current approaches and recent advances in the analytical methods for determining the biomarkers of exposure to pesticides in the most commonly used specimens, such as blood, urine, and breast milk, and in emerging non-invasive matrices such as hair and meconium. We critically discuss the main applications for sample treatment, and the instrumental techniques currently used to determine the most relevant pesticide biomarkers. We finally look at the future trends in this field. Copyright © 2015 Elsevier B.V. All rights reserved.

Biomonitoring at the UK Health and Safety Laboratory.

PubMed

Cocker, J; Jones, K; Morton, J; Mason, H J

2007-05-01

The UK Health and Safety Laboratory (HSL) provides research and analytical support to the Health and Safety Executive, other Government Departments and employers. In the area of biomonitoring HSL conducts research studies and provides an analytical service for regular surveillance of worker exposure to hazardous substances. This paper gives brief examples of how data from such studies can be used to develop biological monitoring guidance values for isocyanates, polycyclic aromatic hydrocarbons and hexavalent chromium. In addition, a study of occupational exposure to copper chrome arsenic wood preservatives is briefly described to show how biological monitoring can be used for post-approval surveillance of a biocide.

Metal concentrations in homing pigeon lung tissue as a biomonitor of atmospheric pollution.

PubMed

Cui, Jia; Halbrook, Richard S; Zang, Shuying; Han, Shuang; Li, Xinyu

2018-03-01

Atmospheric pollution in urban areas is a major worldwide concern with potential adverse impacts on wildlife and humans. Biomonitoring can provide direct evidence of the bioavailability and bioaccumulation of toxic metals in the environment that is not available with mechanical air monitoring. The current study continues our evaluation of the usefulness of homing pigeon lung tissue as a biomonitor of atmospheric pollution. Homing pigeons (1-2, 5-6, and 9-10+ year old (yo)) collected from Guangzhou during 2015 were necropsied and concentrations of cadmium (Cd), lead (Pb), and mercury (Hg) were measured in lung tissue. Lung Cd and Pb concentrations were significantly greater in 9-10+-year-old pigeons compared with those in other age groups, indicating their bioavailability and bioaccumulation. Lung Pb and Cd concentrations measured in 5-yo pigeons collected from Guangzhou during 2015 were significantly lower than concentrations reported in 5-yo homing pigeons collected from Guangzhou during 2011 and correlated with concentrations measured using mechanical air monitoring. In addition to temporal differences, spatial differences in concentrations of Cd, Pb, and Hg reported in ambient air samples and in pigeon lung tissues collected from Beijing and Guangzhou are discussed.

Biological monitoring of carcinogens: current status and perspectives.

PubMed

Pavanello, Sofia; Lotti, Marcello

2012-04-01

Biomonitoring exposures to carcinogens is common practice and a variety of biomarkers have been developed to assess both exposures and biochemical/biological effects. However, their clinical and preventive relevance is still uncertain. The understanding of cancer as a genetic disease has dramatically evolved during last decades, showing that cancer cell types acquire their characteristics with different strategies, time frames and microenvironments. Therefore, the place of current biomarkers within this complex scenario of gene-environment interactions leading to cancer cannot be defined. Reasons are manifold. Most studies assessed cancer risk on a group basis through snapshots taken at unknown time-points of the postulated chain of events. Little attention has been paid to the variety and variability of exposures, and no prospective study validated the indicators of biochemical/biological effects. New opportunities and suggestions for biomonitoring exposures to carcinogens could derive from exploring the exposome that combines exposures from all sources both external and internal. The discovery of new biomarkers and the identification of relevant gene-specific pathways could be achieved through metabolomic and genome-wide studies. In conclusion, it is possible to envisage personalized biomonitoring procedures, such as those already implemented in the context of nutrition and clinical oncology.

POST-REMEDIATION BIOMONITORING OF PESTICIDES AND OTHER CONTAMINANTS IN MARINE WATERS AND SEDIMENT NEAR THE UNITED HECKATHORN SUPERFUND SITE, RICHMOND, CALIFORNIA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Antrim, Liam D.; Kohn, Nancy P.

Marine sediment remediation at the United Heckathorn Superfund Site was completed in April 1997. Water and mussel tissues were sampled in February 1999 from four stations near Lauritzen Canal in Richmond, California, for Year 2 of post-remediation monitoring of marine areas near the United Heckathorn Site. Dieldrin and dichlorodiphenyl trichloroethane (DDT) were analyzed in water samples, tissue samples from resident mussels, and tissue samples from transplanted mussels deployed for 4 months. Mussel tissues were also analyzed for polychlorinated biphenyls (PCB), which were detected in sediment samples. Chlorinated pesticide concentrations in water samples were similar to preremediation levels and did notmore » meet remediation goals. Biomonitoring results indicated that the bioavailability of chlorinated pesticides has been reduced from preremediation levels both in the dredged area and throughout Richmond Harbor. Total DDT and dieldrin concentrations in mussel tissues were lower than measured levels from preremediation surveys and also lower than Year 1 levels from post-remediation biomonitoring. Sediment analyses showed the presence of elevated DDT, dieldrin, PCB aroclor 1254, and very high levels of polynuclear aromatic hydrocarbons (PAH) in Lauritzen Channel.« less

Bringing together raptor collections in Europe for contaminant research and monitoring in relation to chemicals regulations.

PubMed

Movalli, Paola; Dekker, René; Koschorreck, Jan; Treu, Gabriele

2017-11-01

Raptors are good sentinels of environmental contamination and there is good capability for raptor biomonitoring in Europe. Raptor biomonitoring can benefit from natural history museums (NHMs), environmental specimen banks (ESBs) and other collections (e.g. specialist raptor specimen collections). Europe's NHMs, ESBs and other collections hold large numbers of raptor specimens and samples, covering long periods of time. These collections are potentially a valuable resource for contaminant studies over time and space. There are strong needs to monitor contaminants in the environment to support EU and national chemical management. However, data on raptor specimens in NHMs, ESBs and other collections are dispersed, few are digitised, and they are thus not easy to access. Specimen coverage is patchy in terms of species, space and time. Contaminant research with raptors would be facilitated by creating a framework to link relevant collections, digitising all collections, developing a searchable meta-database covering all existing collections, making them more visible and accessible for contaminant research. This would also help identify gaps in coverage and stimulate specimen collection to fill gaps in support of prioritised contaminant monitoring. Collections can further support raptor biomonitoring by making samples available for analysis on request.

Barnacles as biomonitors of trace metal availabilities in Hong Kong coastal waters: changes in space and time.

PubMed

Rainbow, P S; Blackmore, G

2001-06-01

The use of selected organisms as biomonitors of trace metal bioavailabilities allows comparisons to be made over space and time. The concentrations of 11 trace metals (arsenic, cadmium, chromium, cobalt, copper, iron, lead, manganese, nickel, silver, zinc) were measured in the bodies of two barnacle species, Balanus amphitrite and Tetraclita squamosa, from up to 18 littoral sites from Hong Kong coastal waters in April 1998. These data provide evidence on the geographical variation in metal bioavailabilities at this time, and are compared selectively against historical data sets for 1986 and 1989. Geographical variation in bioavailabilities is clear for several metals, with hotspots for arsenic, copper, nickel and silver at Chai Wan Kok, and for lead in Junk Bay. Victoria Harbour sites head the rankings for silver and arsenic, and Tolo Harbour sites exhibit relatively elevated cobalt, manganese and zinc. Many bioavailabilities of trace metals to barnacles are lower in Hong Kong coastal waters in 1998 than in 1986. The two barnacle species are widespread and the extensive data set presented is a benchmark which can be compared to the results of similar biomonitoring programmes elsewhere in the Indo-Pacific and beyond.

Review of 2,4-dichlorophenoxyacetic acid (2,4-D) biomonitoring and epidemiology.

PubMed

Burns, Carol J; Swaen, Gerard M H

2012-10-01

A qualitative review of the epidemiological literature on the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) and health after 2001 is presented. In order to compare the exposure of the general population, bystanders and occupational groups, their urinary levels were also reviewed. In the general population, 2,4-D exposure is at or near the level of detection (LOD). Among individuals with indirect exposure, i.e. bystanders, the urinary 2,4-D levels were also very low except in individuals with opportunity for direct contact with the herbicide. Occupational exposure, where exposure was highest, was positively correlated with behaviors related to the mixing, loading and applying process and use of personal protection. Information from biomonitoring studies increases our understanding of the validity of the exposure estimates used in epidemiology studies. The 2,4-D epidemiology literature after 2001 is broad and includes studies of cancer, reproductive toxicity, genotoxicity, and neurotoxicity. In general, a few publications have reported statistically significant associations. However, most lack precision and the results are not replicated in other independent studies. In the context of biomonitoring, the epidemiology data give no convincing or consistent evidence for any chronic adverse effect of 2,4-D in humans.

Review of 2,4-dichlorophenoxyacetic acid (2,4-D) biomonitoring and epidemiology

PubMed Central

Burns, Carol J.; Swaen, Gerard M. H.

2012-01-01

A qualitative review of the epidemiological literature on the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) and health after 2001 is presented. In order to compare the exposure of the general population, bystanders and occupational groups, their urinary levels were also reviewed. In the general population, 2,4-D exposure is at or near the level of detection (LOD). Among individuals with indirect exposure, i.e. bystanders, the urinary 2,4-D levels were also very low except in individuals with opportunity for direct contact with the herbicide. Occupational exposure, where exposure was highest, was positively correlated with behaviors related to the mixing, loading and applying process and use of personal protection. Information from biomonitoring studies increases our understanding of the validity of the exposure estimates used in epidemiology studies. The 2,4-D epidemiology literature after 2001 is broad and includes studies of cancer, reproductive toxicity, genotoxicity, and neurotoxicity. In general, a few publications have reported statistically significant associations. However, most lack precision and the results are not replicated in other independent studies. In the context of biomonitoring, the epidemiology data give no convincing or consistent evidence for any chronic adverse effect of 2,4-D in humans. PMID:22876750

Epidemiologic studies of glyphosate and cancer: a review.

PubMed

Mink, Pamela J; Mandel, Jack S; Sceurman, Bonnielin K; Lundin, Jessica I

2012-08-01

The United States Environmental Protection Agency and other regulatory agencies around the world have registered glyphosate as a broad-spectrum herbicide for use on multiple food and non-food use crops. Glyphosate is widely considered by regulatory authorities and scientific bodies to have no carcinogenic potential, based primarily on results of carcinogenicity studies of rats and mice. To examine potential cancer risks in humans, we reviewed the epidemiologic literature to evaluate whether exposure to glyphosate is associated causally with cancer risk in humans. We also reviewed relevant methodological and biomonitoring studies of glyphosate. Seven cohort studies and fourteen case-control studies examined the association between glyphosate and one or more cancer outcomes. Our review found no consistent pattern of positive associations indicating a causal relationship between total cancer (in adults or children) or any site-specific cancer and exposure to glyphosate. Data from biomonitoring studies underscore the importance of exposure assessment in epidemiologic studies, and indicate that studies should incorporate not only duration and frequency of pesticide use, but also type of pesticide formulation. Because generic exposure assessments likely lead to exposure misclassification, it is recommended that exposure algorithms be validated with biomonitoring data. Copyright © 2012 Elsevier Inc. All rights reserved.

Evaluating Cumulative OP Pesticide Body Burden of Children: A National Case Study

PubMed Central

Payne-Sturges, Devon; Cohen, Jonathan; Castorina, Rosemary; Axelrad, Daniel A.; Woodruff, Tracey J.

2009-01-01

Biomonitoring is a valuable tool for identifying exposures to chemicals that pose potential harm to human health. However, to date there has been little published on ways to evaluate the relative public health significance of biomonitoring data for different chemicals, and even less on cumulative assessment of multiple chemicals. The objectives of our study are to develop a methodology for a health risk interpretation of biomonitoring data, and to apply it using NHANES 1999–2002 body burden data for organophosphorus (OP) pesticides. OP pesticides present a particularly challenging case given the non-specificity of many metabolites monitored through NHANES. We back-calculate OP pesticide exposures from urinary metabolite data, and compare cumulative dose estimates with available toxicity information for a common mechanism of action (brain cholinesterase inhibition) using data from U.S. EPA. Our results suggest that approximately 40% of children in the United States may have had insufficient margins of exposure (MOEs) for neurological impacts from cumulative exposures to OP pesticides (MOE less than 1,000). Limitations include uncertainty related to assumptions about likely precursor pesticide compounds of the urinary metabolites, sources of exposure, and intra-individual and temporal variability. PMID:19921915

Leaves of Bauhinia blakeana as indicators of atmospheric pollution in Hong Kong

NASA Astrophysics Data System (ADS)

Lau, O. W.; Luk, S. F.

Bauhinia blakeana was used as a biomonitor to monitor the air quality in Hong Kong. Equations were set up to relate the ambient iron, copper, zinc and lead concentrations with those in leaves of the biomonitor and good correlations were observed. The concentration of sulphate in the leaves of Bauhinia blakeana was found to be directly related to ambient sulphur dioxide and total suspended particulates. Using these equations the ambient pollutant levels in different districts of Hong Kong were determined quantitatively according to the concentrations of pollutants in leaves. As many residential buildings are close to congested roads, the ambient pollutant concentrations at selected roads were evaluated. Many temples are known to be heavily polluted with air particulates, and thus the air quality inside are suspected to be poor. The air quality inside temples may be reflected by the air quality outside these buildings, which were also assessed using the proposed method of biomonitoring. The levels of ambient lead and copper outside these temples were higher than their respective background levels while the levels of pollutants at the kerbsides were reported to be 10-300% higher than those of the background.

Characterization of the Etna volcanic emissions through an active biomonitoring technique (moss-bags): part 1--major and trace element composition.

PubMed

Calabrese, S; D'Alessandro, W; Bellomo, S; Brusca, L; Martin, R S; Saiano, F; Parello, F

2015-01-01

Active biomonitoring using moss-bags was applied to an active volcanic environment for the first time. Bioaccumulation originating from atmospheric deposition was evaluated by exposing mixtures of washed and air-dried mosses (Sphagnum species) at 24 sites on Mt. Etna volcano (Italy). Concentrations of major and a large suite of trace elements were analysed by inductively coupled mass and optical spectrometry (ICP-MS and ICP-OES) after total acid digestion. Of the 49 elements analysed those which closely reflect summit volcanic emissions were S, Tl, Bi, Se, Cd, As, Cu, B, Na, Fe, Al. Enrichment factors and cluster analysis allowed clear distinction between volcanogenic, geogenic and anthropogenic inputs that affect the local atmospheric deposition. This study demonstrates that active biomonitoring with moss-bags is a suitable and robust technique for implementing inexpensive monitoring in scarcely accessible and harsh volcanic environments, giving time-averaged quantitative results of the local exposure to volcanic emissions. This task is especially important in the study area because the summit area of Mt. Etna is visited by nearly one hundred thousand tourists each year who are exposed to potentially harmful volcanic emissions. Copyright © 2014 Elsevier Ltd. All rights reserved.

Human biomonitoring pilot study DEMOCOPHES in Germany: Contribution to a harmonized European approach.

PubMed

Schwedler, Gerda; Seiwert, Margarete; Fiddicke, Ulrike; Ißleb, Sissy; Hölzer, Jürgen; Nendza, Julia; Wilhelm, Michael; Wittsiepe, Jürgen; Koch, Holger M; Schindler, Birgit K; Göen, Thomas; Hildebrand, Jörg; Joas, Reinhard; Joas, Anke; Casteleyn, Ludwine; Angerer, Jürgen; Castano, Argelia; Esteban, Marta; Schoeters, Greet; Den Hond, Elly; Sepai, Ovnair; Exley, Karen; Bloemen, Louis; Knudsen, Lisbeth E; Kolossa-Gehring, Marike

2017-06-01

Human biomonitoring (HBM) is an effective tool to assess human exposure to environmental pollutants, but comparable HBM data in Europe are lacking. In order to expedite harmonization of HBM studies on a European scale, the twin projects COPHES (Consortium to Perform Human Biomonitoring on a European Scale) and DEMOCOPHES (Demonstration of a study to Coordinate and Perform Human Biomonitoring on a European Scale) were formed, comprising 35 partners from 27 European countries. In COPHES a research scheme and guidelines were developed to exemplarily measure in a pilot study mercury in hair, cadmium, cotinine and several phthalate metabolites in urine of 6-11year old children and their mothers in an urban and a rural region. Seventeen European countries simultaneously conducted this cross-sectional DEMOCOPHES feasibility study. The German study population was taken in the city of Bochum and in the Higher Sauerland District, comprising 120 mother-child pairs. In the present paper features of the study implementation are presented. German exposure concentrations of the pollutants are reported and compared with European average concentrations from DEMOCOPHES and with those measured in the representative German Environmental Survey (GerES IV). German DEMOCOPHES concentrations for mercury and cotinine were lower than the European average. However, 47% of the children were still exposed to environmental tobacco smoke (ETS) outside their home, which gives further potential for enhancing protection of children from ETS. Compared with samples from the other European countries German participating children had lower concentrations of the phthalate metabolites MEP and of the sum of 3 DEHP-metabolites (MEHP, 5OH-MEHP and 5oxo-MEHP), about the same concentrations of the phthalate metabolites MBzP and MiBP and higher concentrations of the phthalate metabolite MnBP. 2.5% of the German children had concentrations of the sum of 4 DEHP-metabolites and 4.2% had concentrations of MnBP that exceeded health based guidance values, indicating reasons for concern. Continuous HBM is necessary to track changes of pollutant exposure over time. Therefore Germany will continue to cooperate on the harmonisation of European human biomonitoring to support the chemicals regulation with the best possible exposure data to protect Europe's people against environmental health risks. Copyright © 2017 The Authors. Published by Elsevier GmbH.. All rights reserved.

Persistent organic pollutants in atmospheric deposition and biomonitoring with Tillandsia usneoides (L.) in an industrialized area in Rio de Janeiro state, southeast Brazil--Part II: PCB and PAH.

PubMed

de Souza Pereira, Márcia; Heitmann, Dieter; Reifenhäuser, Werner; Meire, Rodrigo Ornellas; Santos, Luciana Silva; Torres, João Paulo M; Malm, Olaf; Körner, Wolfgang

2007-04-01

Monitoring of immission of persistent organic pollutants in the industrialized area of Volta Redonda (V.R.) and in the National Park of Itatiaia (PNI) in southeast Brazil was performed using an endemic bromeliad species as biomonitor and measuring bulk deposition rates of polychlorinated biphenyls (PCB) and polycyclic aromatic hydrocarbons (PAH). For the sum of PCB, overall deposition rates were between 17 and 314 ng/(m2 day) in winter and between 43 and 81 ng/(m2 day) in summer, respectively. Deposition rates of dioxin-like PCBs ranged from 0.14 to 2.8 pg WHO-TEQ/(m2 day) in winter and from 0.90 to 4.3 pg WHO-TEQ/(m2 day) in summer. PCB deposition rates (total PCB and WHO-TEQ) were in the same range in winter in V.R. and PNI. In summer, contamination levels in V.R. were 6-10-folds higher than in PNI. PCB concentrations in biomonitor samples from V.R. and PNI were in the same range in summer and in winter. Concentrations of total PCB ranged from 14 to 95 microg/kg dry matter (d.m.) in winter and from 18 to 27 microg/kg d.m. in summer, respectively. The TEQ values were between 1.7 and 4.1 ng WHO-TEQ/kg d.m. in winter and between 1.9 and 2.9 ng WHO-TEQ/kg d.m. in summer. PCB concentrations of di-ortho PCB but not of non-ortho PCB were a factor of 2-4 lower in summer in both areas. PCB congener profiles resembled those from technical formulations. The profiles shifted to the higher chlorinated congeners in summer, probable due to revolatilisation of the lighter components at higher temperatures. PCB profiles in biomonitor resembled those from deposition samples and the shift to the heavier congeners in summer was even more pronounced. PAH deposition rates were in a similar range in both areas (131-2415 ng/(m2 day)). PAH levels in biomonitor samples from V.R. were about one order of magnitude higher than in samples from PNI indicating the impact of local sources. PAH profiles revealed stationary thermal processes as main source of contamination in V.R. whereas in PNI, biomass burning seems to be the main contamination source.

Ground Testing of Prototype Hardware and Processing Algorithms for a Wide Area Space Surveillance System (WASSS)

DTIC Science & Technology

2013-09-01

Ground testing of prototype hardware and processing algorithms for a Wide Area Space Surveillance System (WASSS) Neil Goldstein, Rainer A...at Magdalena Ridge Observatory using the prototype Wide Area Space Surveillance System (WASSS) camera, which has a 4 x 60 field-of-view , < 0.05...objects with larger-aperture cameras. The sensitivity of the system depends on multi-frame averaging and a Principal Component Analysis based image

A prototype space flight intravenous injection system

NASA Technical Reports Server (NTRS)

Colombo, G. V.

1985-01-01

Medical emergencies, especially those resulting from accidents, frequently require the administration of intravenous fluids to replace lost body liquids. The development of a prototype space flight intravenous injection system is presented. The definition of requirements, injectable concentrates development, water polisher, reconstitution hardware development, administration hardware development, and prototype fabrication and testing are discussed.

A Prototype HTML Training System for Graphic Communication Majors

ERIC Educational Resources Information Center

Runquist, Roger L.

2010-01-01

This design research demonstrates a prototype content management system capable of training graphic communication students in the creation of basic HTML web pages. The prototype serve as a method of helping students learn basic HTML structure and commands earlier in their academic careers. Exposure to the concepts of web page creation early in…

Mockup design of personal health diary app for patients with chronic kidney disease.

PubMed

Lin, Hsiu-Wen; Wang, Yu-Jen; Jing, Ling-Fang; Chang, Polun

2014-01-01

Health self-management is important in the care of patients with chronic kidney disease. It is possible to improve the efficiency of patient self-management through the use of mobile technology and related software. This study is divided into three stages: 1. analysis of need: through observation, interview and content analysis of the chronic kidney disease health management manual; 2. design of system prototype: establish interface and system function; 3. prototype evaluation: evaluate whether the prototype designed by this study meets user needs. The system prototype includes: daily record, laboratory examination results, trend graphs, information search, sharing, communications and settings. Prototyping is done with Pencil Project for interface design and linking. The prototype is then exported in PDF format for mock-up simulation. Evaluation results: overall score was 4.01±0.60 leaning towards "agree", the highest score was ease of use (4.25±0.6), followed by easy to learn (4.15±0.68), acceptance (4.01±0.61), reliability (3.87±0.6) and functionality (3.83±0.49). The results show positive attitude towards the system.

Geographic information systems - transportation ISTEA management systems server net prototype pooled fund study : phase B - summary

DOT National Transportation Integrated Search

1997-06-01

The Geographic Information System-Transportation (GIS-T) ISTEA Management Systems Server Net Prototype Pooled Fund Study represents the first national cooperative effort in the transportation industry to address the management and monitoring systems ...

Prototype development and demonstration for response, emergency staging, communications, uniform management, and evacuation (R.E.S.C.U.M.E.) : R.E.S.C.U.M.E. prototype system architecture.

DOT National Transportation Integrated Search

2014-01-01

This document provides the high-level system architecture for the Prototype Development and Demonstration of a R.E.S.C.U.M.E. system. The requirements addressed in this document are based upon those that can be found in previous R.E.S.C.U.M.E. report...

Atmospheric Correction Prototype Algorithm for High Spatial Resolution Multispectral Earth Observing Imaging Systems

NASA Technical Reports Server (NTRS)

Pagnutti, Mary

2006-01-01

This viewgraph presentation reviews the creation of a prototype algorithm for atmospheric correction using high spatial resolution earth observing imaging systems. The objective of the work was to evaluate accuracy of a prototype algorithm that uses satellite-derived atmospheric products to generate scene reflectance maps for high spatial resolution (HSR) systems. This presentation focused on preliminary results of only the satellite-based atmospheric correction algorithm.

Prototype solar heating and combined heating cooling systems

NASA Technical Reports Server (NTRS)

1978-01-01

The design and development of eight prototype solar heating and combined heating and cooling systems is discussed. The program management and systems engineering are reported, and operational test sites are identified.

Research into display sharing techniques for distributed computing environments

NASA Technical Reports Server (NTRS)

Hugg, Steven B.; Fitzgerald, Paul F., Jr.; Rosson, Nina Y.; Johns, Stephen R.

1990-01-01

The X-based Display Sharing solution for distributed computing environments is described. The Display Sharing prototype includes the base functionality for telecast and display copy requirements. Since the prototype implementation is modular and the system design provided flexibility for the Mission Control Center Upgrade (MCCU) operational consideration, the prototype implementation can be the baseline for a production Display Sharing implementation. To facilitate the process the following discussions are presented: Theory of operation; System of architecture; Using the prototype; Software description; Research tools; Prototype evaluation; and Outstanding issues. The prototype is based on the concept of a dedicated central host performing the majority of the Display Sharing processing, allowing minimal impact on each individual workstation. Each workstation participating in Display Sharing hosts programs to facilitate the user's access to Display Sharing as host machine.

A conceptual model for megaprogramming

NASA Technical Reports Server (NTRS)

Tracz, Will

1990-01-01

Megaprogramming is component-based software engineering and life-cycle management. Magaprogramming and its relationship to other research initiatives (common prototyping system/common prototyping language, domain specific software architectures, and software understanding) are analyzed. The desirable attributes of megaprogramming software components are identified and a software development model and resulting prototype megaprogramming system (library interconnection language extended by annotated Ada) are described.

Prototype active scanner for nighttime oil spill mapping and classification

NASA Technical Reports Server (NTRS)

Sandness, G. A.; Ailes, S. B.

1977-01-01

A prototype, active, aerial scanner system was constructed for nighttime water pollution detection and nighttime multispectral imaging of the ground. An arc lamp was used to produce the transmitted light and four detector channels provided a multispectral measurement capability. The feasibility of the design concept was demonstrated by laboratory and flight tests of the prototype system.

Form Factor Evaluation of Open Body Area Network (OBAN) Physiological Status Monitoring (PSM) System Prototype Designs

DTIC Science & Technology

2018-05-11

SYSTEM PROTOTYPE DESIGNS DISCLAIMERS The opinions or assertions contained herein are the private views of the author(s) and are not to be construed as...FORM FACTOR EVALUATION OF OPEN BODY AREA NETWORK (OBAN) PHYSIOLOGICAL STATUS MONITORING (PSM) SYSTEM PROTOTYPE DESIGNS William J...security; and is designed to function for 72 hours or more. The test described in this report assesses proposed form-factor designs . Feedback using

Unmanned Aircraft Systems (UAS) Integration in the National Airspace System (NAS) Project

NASA Technical Reports Server (NTRS)

Griner, James H.

2013-01-01

NASA's UAS Integration in the NAS project, has partnered with Rockwell Collins to develop a concept Control and Non-Payload Communication system prototype radio, operating on recently allocated UAS frequency spectrum bands. The prototype radio will be used to validate initial proposed performance requirements for UAS control communications. This presentation will give an overview of the current status of the design, development, and flight test planning for this prototype radio.

Unmanned Aircraft Systems (UAS) Integration in the National Airspace System (NAS) Project - Gen-4 and Gen-5 Radio Plans

NASA Technical Reports Server (NTRS)

Griner, James H.

2014-01-01

NASA's UAS Integration in the NAS project, has partnered with Rockwell Collins to develop a concept Control and Non-Payload Communication (CNPC) system prototype radio, operating on recently allocated UAS frequency spectrum bands. This prototype radio is being used to validate initial proposed performance requirements for UAS control communications. This presentation will give an overview of the current plans for the prototype radio development.

NIMH Prototype Management Information System for Community Mental Health Centers

PubMed Central

Wurster, Cecil R.; Goodman, John D.

1980-01-01

Various approaches to centralized support of computer applications in health care are described. The NIMH project to develop a prototype Management Information System (MIS) for community mental health centers is presented and discussed as a centralized development of an automated data processing system for multiple user organizations. The NIMH program is summarized, the prototype MIS is characterized, and steps taken to provide for the differing needs of the mental health centers are highlighted.

Rapid Prototyping: An Alternative Instructional Design Strategy.

ERIC Educational Resources Information Center

Tripp, Steven D.; Bichelmeyer, Barbara

1990-01-01

Discusses the nature of instructional design and describes rapid prototyping as a feasible model for instructional system design (ISD). The use of prototyping in software engineering is described, similarities between software design and instructional design are discussed, and an example is given which uses rapid prototyping in designing a…

Management advisory memorandum on National Airspace System infrastructure management system prototype, Federal Aviation Administration

DOT National Transportation Integrated Search

1997-03-01

This is our Management Advisory Memorandum on the National Airspace : System (NAS) Infrastructure Management System (NIMS) prototype : project in the Federal Aviation Administration (FAA). Our review was : initiated in response to a hotline complaint...

Heavy metal biomonitoring and phytoremediation potentialities of aquatic macrophytes in River Nile.

PubMed

Fawzy, Manal Ahmed; Badr, Nadia El-sayed; El-Khatib, Ahmed; Abo-El-Kassem, Amany

2012-03-01

The concentrations of Cd, Cu, Pb, and Zn in sediments, water, and different plant organs of six aquatic vascular plant species, Ceratophyllum demersum L. Echinochloa pyramidalis (Lam.) Hitchc. & Chase; Eichhornia crassipes (Mart.) Solms-Laub; Myriophyllum spicatum L.; Phragmites australis (Cav.) Trin. ex Steud; and Typha domingensis (Pers.) Poir. ex Steud, growing naturally in the Nile system (Sohag Governorate), were investigated. The aim was to define which species and which plant organs exhibit the greatest accumulation and evaluate whether these species could be usefully employed in biomonitoring and phytoremediation programs. The recorded metals in water samples were above the standard levels of both US Environmental Protection Agency and Egyptian Environmental Affairs Agency except for Pb. The concentrations of heavy metals in water, sediments, and plants possess the same trend: Zn > Cu > Pb > Cd which reflects the biomonitoring potentialities of the investigated plant species. Generally, the variation of heavy element concentrations in water and sediments in relation to site and season, as assessed by two-way repeated measured ANOVA, was significant (p < 0.05). However, insignificant variations were observed in the concentrations of Pb and Cd in sediments in relation to season and of Cu and Zn in relation to site. Results also showed that the selectivity of the heavy elements for the investigated plants varied significantly (p < 0.05) with species variation. The accumulation capability of the investigated species could be arranged according to this pattern: C. demersum > E. crassipes > M. spicatum > E. pyramidalis > T. domingensis > P. australis. On the basis of the element concentrations, roots of all the studied species contain higher concentrations of Cu and Zn than shoots while leaves usually acquire the highest concentrations of Pb. Cd concentrations among different plant organs are comparable except in M. spicatum where the highest Cd concentrations were recorded in the leaves. Our results also demonstrated that all the studied species can accumulate more than 1,450-fold the concentration of the investigated heavy elements in water rendering them of interest for use in phytoremediation studies of polluted waters. Given the absence of systematic water quality monitoring, heavy elements in plants, rather than sediments, provide a cost-effective means for assessing heavy element accumulation in aquatic systems during plant organ lifespan.

SP-100 GES/NAT radiation shielding systems design and development testing

NASA Astrophysics Data System (ADS)

Disney, Richard K.; Kulikowski, Henry D.; McGinnis, Cynthia A.; Reese, James C.; Thomas, Kevin; Wiltshire, Frank

1991-01-01

Advanced Energy Systems (AES) of Westinghouse Electric Corporation is under subcontract to the General Electric Company to supply nuclear radiation shielding components for the SP-100 Ground Engineering System (GES) Nuclear Assembly Test to be conducted at Westinghouse Hanford Company at Richland, Washington. The radiation shielding components are integral to the Nuclear Assembly Test (NAT) assembly and include prototypic and non-prototypic radiation shielding components which provide prototypic test conditions for the SP-100 reactor subsystem and reactor control subsystem components during the GES/NAT operations. W-AES is designing three radiation shield components for the NAT assembly; a prototypic Generic Flight System (GFS) shield, the Lower Internal Facility Shield (LIFS), and the Upper Internal Facility Shield (UIFS). This paper describes the design approach and development testing to support the design, fabrication, and assembly of these three shield components for use within the vacuum vessel of the GES/NAT. The GES/NAT shields must be designed to operate in a high vacuum which simulates space operations. The GFS shield and LIFS must provide prototypic radiation/thermal environments and mechanical interfaces for reactor system components. The NAT shields, in combination with the test facility shielding, must provide adequate radiation attenuation for overall test operations. Special design considerations account for the ground test facility effects on the prototypic GFS shield. Validation of the GFS shield design and performance will be based on detailed Monte Carlo analyses and developmental testing of design features. Full scale prototype testing of the shield subsystems is not planned.

Prototype solar heating and combined heating and cooling systems

NASA Technical Reports Server (NTRS)

1976-01-01

Eight prototype solar heating and combined heating and cooling systems are considered. This effort includes development, manufacture, test, installation, maintenance, problem resolution, and performance evaluation.

Prototype solar heating and cooling systems

NASA Technical Reports Server (NTRS)

1978-01-01

A collection of monthly status reports on the development of eight prototype solar heating and cooling systems is presented. The effort calls for the development, manufacture, test, system installation, maintenance, problem resolution, and performance evaluation. The systems are 3, 25, and 75 ton size units.

Prototype solar heating and cooling systems

NASA Technical Reports Server (NTRS)

1978-01-01

A collection of monthly status reports are given on the development of eight prototype solar heating and cooling systems. This effort calls for the development, manufacturing, test, system installation, maintenance, problem resolution, and performance evaluation. The systems are 3-, 25-, and 75-ton size units.

SIMS prototype system 3 test results: Engineering analysis

NASA Technical Reports Server (NTRS)

1978-01-01

The results obtained during testing of a closed hydronic drain down solar system designed for space and hot water heating is presented. Data analysis is included which documents the system performance and verifies the suitability of SIMS Prototype System 3 for field installation.

Progress in the Development of Direct Osmotic Concentration Wastewater Recovery Process for Advanced Life Support Systems

NASA Technical Reports Server (NTRS)

Cath, Tzahi Y.; Adams, Dean V.; Childress, Amy; Gormly, Sherwin; Flynn, Michael

2005-01-01

Direct osmotic concentration (DOC) has been identified as a high potential technology for recycling of wastewater to drinking water in advanced life support (ALS) systems. As a result the DOC process has been selected for a NASA Rapid Technology Development Team (RTDT) effort. The existing prototype system has been developed to a Technology Readiness Level (TRL) 3. The current project focuses on advancing the development of this technology from TRL 3 to TRL 6 (appropriate for human rated testing). A new prototype of a DOC system is been designed and fabricated that addresses the deficiencies encountered during the testing of the original system and allowing the new prototype to achieve TRL 6. Background information is provided about the technologies investigated and their capabilities, results from preliminary tests, and the milestones plan and activities for the RTDT program intended to develop a second generation prototype of the DOC system.

Biomonitoring potential of five sympatric Tillandsia species for evaluating urban metal pollution (Cd, Hg and Pb)

NASA Astrophysics Data System (ADS)

Sánchez-Chardi, Alejandro

2016-04-01

The present study quantifies non essential heavy metals highly toxic for biological systems (Pb, Hg and Cd) in five autochthonous epiphytic plants from Tillandsia genus (T. recurvata, T. meridionalis, T. duratii, T. tricholepis, T. loliacea) according to different traffic levels (reference, low, medium and high polluted sites) in Asunción (Paraguay). The three metals increased in polluted sites following Pb (till 62.99 ppm in T. tricholepis) > Cd (till 1.35 ppm in T. recurvata) > Hg (till 0.36 ppm in T. recurvata) and Pb and Cd levels were directly related to traffic flow. Although the species showed similar bioaccumulation pattern (namely, higher levels of metals in polluted sites), enrichment factors (maximum EF values 37.00, 18.16, and 11.90 for Pb, Hg, and Cd, respectively) reported T. tricholepis as the most relevant bioindicator due to its wide distribution and abundance in study sites, low metal content in control site and high metal contents in polluted sites, and significant correlations with traffic density of Pb and Cd. This study emphasizes the necessity of biomonitoring air pollution in areas out of air monitoring control such as Asunción, where the high levels of metal pollution especially Pb, may represent an increment of risk for the human population inhabiting this urban area.

Soil genotoxicity assessment: a new stategy based on biomolecular tools and plant bioindicators.

PubMed

Citterio, Sandra; Aina, Roberta; Labra, Massimo; Ghiani, Alessandra; Fumagalli, Pietro; Sgorbati, Sergio; Santagostino, Angela

2002-06-15

The setting up of efficient early warning systems is a challenge to research for preventing environmental alteration and human disease. In this paper, we report the development and the field application of a new biomonitoring methodology for assessing soil genotoxicity. In the first part, the use of amplified fragment length polymorphism and flow cytometry techniques to detect DNA damage induced by soils artificially contaminated with heavy metals as potentially genotoxic compounds is explained. Results show that the combination of the two techniques leads to efficient detection of the sublethal genotoxic effect induced in the plant bioindicator by contaminated soil. By contrast, the classic mortality, root, and shoot growth vegetative endpoints prove inappropriate for assessing soil genotoxicity because, although they cause genotoxic damage, some heavy metals do not affect sentinel plant development negatively. The statistical elaboration of the data obtained led to the development of a statistical predictive model which differentiates four different levels of soil genotoxic pollution and can be used everywhere. The second part deals with the application of the biomonitoring protocol in the genotoxic assessment of two areas surrounding a steelworks in northern Italy and the effectiveness of this methodology. In this particular case, in these areas, the predictive model reveals a pollution level strictly correlated to the heavy metal concentrations revealed by traditional chemical analysis.

Biomonitoring of Environmental Status and Trends (BEST) Program: selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems

USGS Publications Warehouse

Schmitt, Christopher J.; Dethloff, Gail M.

2000-01-01

This document describes the suite of biological methods of the U.S. Geological Survey- Biomonitoring of Environmental Status and Trends program for monitoring chemical contaminants and their effects on fish. The methods, which were selected by panels of experts, are being field-tested in rivers of the Mississippi River, Columbia River, and Rio Grande basins. General health biomarkers include a health assessment index based on gross observation; histopathological examination of selected organs and tissues; condition factor; and the heptosomatic and splenosomatic indices. Immune system indicators are plasma lysozyme activity and measures of splenic macrophage aggregates. Reproductive biomarkers include plasma concentrations of sex steroid hormones (17b-estradiol and 11-ketotestosterone) and vitellogenin, gonadal histopathology (including reproductive stage and, in females, gonadal atresia), and the gonadosomatic index. Indicators of exposure to polycyclic aromatic and polyhalogenated hydrocarbons are the H4IIE rat hepatoma cell bioassay (performed on solvent extracts of composite fish samples) and hepatic ethoxyresorufin-O-deethylase activity. Stable nitrogen isotope ratios are used to assess the trophic position of the fish and their exposure to sewage and other animal wastes. For each indicator we describe endpoint(s) and methods, and discuss the indicator?s value and limitations for contaminant monitoring and assessment.

Indoor air condensate as a novel matrix for monitoring inhalable organic contaminants.

PubMed

Roll, Isaac B; Halden, Rolf U; Pycke, Benny F G

2015-05-15

With the population of developed nations spending nearly 90% of their time indoors, indoor air quality (IAQ) is a critical indicator of human health risks from inhalation of airborne contaminants. We present a novel approach for qualitative monitoring of IAQ through the collection and analysis of indoor air condensate discharged from heat exchangers of heating, ventilation, and air conditioning (HVAC) systems. Condensate samples were collected from six suburban homes and one business in Maricopa County, Arizona, concentrated via solid-phase extraction, analyzed for 10 endocrine disrupting chemicals (EDCs) by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and screened for additional organic compounds by gas chromatography-mass spectrometry (GC-MS). All 10 EDCs were detected in at least one of the sampled buildings. More than 100 additional compounds were detected by GC-MS, of which 40 were tentatively identified using spectral database searches. Twelve compounds listed as designated chemicals for biomonitoring by the California Environmental Contaminant Biomonitoring Program were detected. Microfiltration of condensate samples prior to extraction had no discernable effect on contaminant concentration, suggesting that contaminants were freely dissolved or associated with inhalable, submicron particles. This study is the first to document the utility of HVAC condensate for the qualitative assessment of indoor air for pollutants. Copyright © 2015 Elsevier B.V. All rights reserved.

A prototype case-based reasoning human assistant for space crew assessment and mission management

NASA Technical Reports Server (NTRS)

Owen, Robert B.; Holland, Albert W.; Wood, Joanna

1993-01-01

We present a prototype human assistant system for space crew assessment and mission management. Our system is based on case episodes from American and Russian space missions and analog environments such as polar stations and undersea habitats. The general domain of small groups in isolated and confined environments represents a near ideal application area for case-based reasoning (CBR) - there are few reliable rules to follow, and most domain knowledge is in the form of cases. We define the problem domain and outline a unique knowledge representation system driven by conflict and communication triggers. The prototype system is able to represent, index, and retrieve case studies of human performance. We index by social, behavioral, and environmental factors. We present the problem domain, our current implementation, our research approach for an operational system, and prototype performance and results.

Prototype development and demonstration for response, emergency staging, communications, uniform management, and evacuation (R.E.S.C.U.M.E.) : R.E.S.C.U.M.E. prototype system design document.

DOT National Transportation Integrated Search

2014-04-01

This report documents the System Design Document (SDD) for the prototype development and demonstration of the Response, Emergency Staging, Communications, Uniform Management, and Evacuation (R.E.S.C.U.M.E.) application bundle, with a focus on the Inc...

Biomonitoring of Air Condition in Urban Environment (with Regard to Irkutsk, Irkutsk region in Russia)

NASA Astrophysics Data System (ADS)

Belykh, L. I.

2017-11-01

The paper deals with the results of investigation into the contents and distribution of cancer-causing benzo(a)pyrene in the leaves of the trees that are common in the urban environment of Irkutsk. The degree of plant pollution is determined depending on the landscape and traffic exposure, on the mode of the plant material being prepared and on its type. The methodology for landscape geochemical biomonitoring is offered to be used at the stages of sample sorting and preparation and in the statistical treatment of the analysis results obtained. The possibility to use tree leaves as biotest materials for the urban atmosphere state control is considered in the paper.

Leaf reflectance variation along a vertical crown gradient of two deciduous tree species in a Belgian industrial habitat.

PubMed

Khavaninzadeh, Ali Reza; Veroustraete, Frank; Van Wittenberghe, Shari; Verrelst, Jochem; Samson, Roeland

2015-09-01

The reflectometry of leaf asymmetry is a novel approach in the bio-monitoring of tree health in urban or industrial habitats. Leaf asymmetry responds to the degree of environmental pollution and reflects structural changes in a leaf due to environmental pollution. This paper describes the boundary conditions to scale up from leaf to canopy level reflectance, by describing the variability of adaxial and abaxial leaf reflectance, hence leaf asymmetry, along the crown height gradients of two tree species. Our findings open a research pathway towards bio-monitoring based on the airborne remote sensing of tree canopies and their leaf asymmetric properties. Copyright © 2015 Elsevier Ltd. All rights reserved.

Design specifications for NALDA (Naval Aviation Logistics Data Analysis) CAI (computer aided instruction): Phase 2, Interim report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Twitty, A.F.; Handler, B.H.; Duncan, L.D.

Data Systems Engineering Organization (DSEO) personnel are developing a prototype computer aided instruction (CAI) system for the Naval Aviation Logistics Data Analysis (NALDA) system. The objective of this project is to provide a prototype for implementing CAI as an enhancement to existing NALDA training. The CAI prototype project is being performed in phases. The task undertaken in Phase I was to analyze the problem and the alternative solutions and to develop a set of recommendations on how best to proceed. In Phase II a structured design and specification document was completed that will provide the basis for development and implementationmore » of the desired CAI system. Phase III will consist of designing, developing, and testing a user interface which will extend the features of the Phase II prototype. The design of the CAI prototype has followed a rigorous structured analysis based on Yourdon/DeMarco methodology and Information Engineering tools. This document includes data flow diagrams, a data dictionary, process specifications, an entity-relationship diagram, a curriculum description, special function key definitions, and a set of standards developed for the NALDA CAI Prototype.« less

Development of compact fuel processor for 2 kW class residential PEMFCs

NASA Astrophysics Data System (ADS)

Seo, Yu Taek; Seo, Dong Joo; Jeong, Jin Hyeok; Yoon, Wang Lai

Korea Institute of Energy Research (KIER) has been developing a novel fuel processing system to provide hydrogen rich gas to residential polymer electrolyte membrane fuel cells (PEMFCs) cogeneration system. For the effective design of a compact hydrogen production system, the unit processes of steam reforming, high and low temperature water gas shift, steam generator and internal heat exchangers are thermally and physically integrated into a packaged hardware system. Several prototypes are under development and the prototype I fuel processor showed thermal efficiency of 73% as a HHV basis with methane conversion of 81%. Recently tested prototype II has been shown the improved performance of thermal efficiency of 76% with methane conversion of 83%. In both prototypes, two-stage PrOx reactors reduce CO concentration less than 10 ppm, which is the prerequisite CO limit condition of product gas for the PEMFCs stack. After confirming the initial performance of prototype I fuel processor, it is coupled with PEMFC single cell to test the durability and demonstrated that the fuel processor is operated for 3 days successfully without any failure of fuel cell voltage. Prototype II fuel processor also showed stable performance during the durability test.

The AS (Administrative Sciences) Financial Reporting System: Some Experience on Prototyping and User Interaction.

DTIC Science & Technology

1986-03-27

RD-RI69 841 THE AS (ADMINISTRATIVE SCIENCES) FINANCIAL REPORTING i4 SYSTEN: SOME EXPERIE.. (U) NAVAL POSTGRADUATE SCHOOL MONTEREY CA R L BOOKER 27...Monterey, California * II LECp THESIS THE AS FINANCIAL REPORTING SYSTEM: SOME EXPERIENCE ON PROTOTYPING AND USER INTERACTION by Ronald L. Booker March...7ASK WORK UNIT ELEMENT NO. NO. NO ACCESSION NO ’ITITLE (Include Security Cassfication) THE AS FINANCIAL REPORTING SYSTEM: SOME EXPERIENCE ON PROTOTYPING

Ground Systems Development Environment (GSDE) interface requirements and prototyping plan

NASA Technical Reports Server (NTRS)

Church, Victor E.; Philips, John; Bassman, Mitchell; Williams, C.

1990-01-01

This report describes the data collection and requirements analysis effort of the Ground System Development Environment (GSDE) Interface Requirements study. It identifies potential problems in the interfaces among applications and processors in the heterogeneous systems that comprises the GSDE. It describes possible strategies for addressing those problems. It also identifies areas for further research and prototyping to demonstrate the capabilities and feasibility of those strategies and defines a plan for building the necessary software prototypes.

An approach for assessing software prototypes

NASA Technical Reports Server (NTRS)

Church, V. E.; Card, D. N.; Agresti, W. W.; Jordan, Q. L.

1986-01-01

A procedure for evaluating a software prototype is presented. The need to assess the prototype itself arises from the use of prototyping to demonstrate the feasibility of a design or development stategy. The assessment procedure can also be of use in deciding whether to evolve a prototype into a complete system. The procedure consists of identifying evaluations criteria, defining alterative design approaches, and ranking the alternatives according to the criteria.

Prototype solar heating and combined heating and cooling systems

NASA Technical Reports Server (NTRS)

1977-01-01

Eight prototype solar heating and combined heating and cooling systems are being developed. The effort includes development, manufacture, test, installation, maintenance, problem resolution, and performance evaluation.

A User Interface for Multiple Retrieval Systems.

ERIC Educational Resources Information Center

Teskey, Niall; And Others

1987-01-01

Reviews current systems designed to help end-users search online databases without the assistance of an intermediary and describes a prototype system which emulates the Deco (the text storage and retrieval system used by Unilever) interface on Dialog and Data-Star. Initial trials of the prototype system are reported. (15 references) (MES)

Adaptation of Control Center Software to Commerical Real-Time Display Applications

NASA Technical Reports Server (NTRS)

Collier, Mark D.

1994-01-01

NASA-Marshall Space Flight Center (MSFC) is currently developing an enhanced Huntsville Operation Support Center (HOSC) system designed to support multiple spacecraft missions. The Enhanced HOSC is based upon a distributed computing architecture using graphic workstation hardware and industry standard software including POSIX, X Windows, Motif, TCP/IP, and ANSI C. Southwest Research Institute (SwRI) is currently developing a prototype of the Display Services application for this system. Display Services provides the capability to generate and operate real-time data-driven graphic displays. This prototype is a highly functional application designed to allow system end users to easily generate complex data-driven displays. The prototype is easy to use, flexible, highly functional, and portable. Although this prototype is being developed for NASA-MSFC, the general-purpose real-time display capability can be reused in similar mission and process control environments. This includes any environment depending heavily upon real-time data acquisition and display. Reuse of the prototype will be a straight-forward transition because the prototype is portable, is designed to add new display types easily, has a user interface which is separated from the application code, and is very independent of the specifics of NASA-MSFC's system. Reuse of this prototype in other environments is a excellent alternative to creation of a new custom application, or for environments with a large number of users, to purchasing a COTS package.

Electronics for a highly segmented electromagnetic calorimeter prototype

NASA Astrophysics Data System (ADS)

Fehlker, D.; Alme, J.; van den Brink, A.; de Haas, A. P.; Nooren, G.-J.; Reicher, M.; Röhrich, D.; Rossewij, M.; Ullaland, K.; Yang, S.

2013-03-01

A prototype of a highly segmented electromagnetic calorimeter has been developed. The detector tower is made of 24 layers of PHASE2/MIMOSA23 silicon sensors sandwiched between tungsten plates, with 4 sensors per layer, a total of 96 MIMOSA sensors, resulting in 39 MPixels for the complete prototype detector tower. The paper focuses on the electronics of this calorimeter prototype. Two detector readout and control systems are used, each containing two Spartan 6 and one Virtex 6 FPGA, running embedded Linux, each system serving 12 detector layers. In 550 ms a total of 4 Gbytes of data is read from the detector, stored in memory on the electronics and then shipped to the DAQ system via Gigabit ethernet.

Unmanned Aircraft Systems (UAS) Integration in the National Airspace System (NAS) Project, UAS Control and Non-Payload Communication System Phase-1 Flight Test Results

NASA Technical Reports Server (NTRS)

Griner, James H.

2014-01-01

NASA's UAS Integration in the NAS project, has partnered with Rockwell Collins to develop a concept Control and Non-Payload Communication (CNPC) system prototype radio, operating on recently allocated UAS frequency spectrum bands. This prototype radio is being used to validate initial proposed performance requirements for UAS control communications. This presentation will give an overview of the current status of the prototype radio development, and results from phase 1 flight tests conducted during 2013.

ALICE inner tracking system readout electronics prototype testing with the CERN "Giga Bit Transceiver''

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schambach, Joachim; Rossewij, M. J.; Sielewicz, K. M.

The ALICE Collaboration is preparing a major detector upgrade for the LHC Run 3, which includes the construction of a new silicon pixel based Inner Tracking System (ITS). The ITS readout system consists of 192 readout boards to control the sensors and their power system, receive triggers, and deliver sensor data to the DAQ. To prototype various aspects of this readout system, an FPGA based carrier board and an associated FMC daughter card containing the CERN Gigabit Transceiver (GBT) chipset have been developed. Furthermore, this contribution describes laboratory and radiation testing results with this prototype board set.

ALICE inner tracking system readout electronics prototype testing with the CERN "Giga Bit Transceiver''

DOE PAGES

Schambach, Joachim; Rossewij, M. J.; Sielewicz, K. M.; ...

2016-12-28

The ALICE Collaboration is preparing a major detector upgrade for the LHC Run 3, which includes the construction of a new silicon pixel based Inner Tracking System (ITS). The ITS readout system consists of 192 readout boards to control the sensors and their power system, receive triggers, and deliver sensor data to the DAQ. To prototype various aspects of this readout system, an FPGA based carrier board and an associated FMC daughter card containing the CERN Gigabit Transceiver (GBT) chipset have been developed. Furthermore, this contribution describes laboratory and radiation testing results with this prototype board set.

ALICE inner tracking system readout electronics prototype testing with the CERN ``Giga Bit Transceiver''

NASA Astrophysics Data System (ADS)

Schambach, J.; Rossewij, M. J.; Sielewicz, K. M.; Aglieri Rinella, G.; Bonora, M.; Ferencei, J.; Giubilato, P.; Vanat, T.

2016-12-01

The ALICE Collaboration is preparing a major detector upgrade for the LHC Run 3, which includes the construction of a new silicon pixel based Inner Tracking System (ITS). The ITS readout system consists of 192 readout boards to control the sensors and their power system, receive triggers, and deliver sensor data to the DAQ. To prototype various aspects of this readout system, an FPGA based carrier board and an associated FMC daughter card containing the CERN Gigabit Transceiver (GBT) chipset have been developed. This contribution describes laboratory and radiation testing results with this prototype board set.

System design package for SIMS prototype system 2, solar hot water

NASA Technical Reports Server (NTRS)

1977-01-01

Information necessary to evaluate the design and assembly of a solar hot water system is presented. A prototype system designed for use in a single family dwelling is investigated in terms of the following subsystems: collector, storage, energy transport, and control.

Freight Advanced Traveler Information System (FRATIS) – Dallas-Fort Worth : as-built system architecture and design.

DOT National Transportation Integrated Search

2015-03-01

This document describes the As-Built System Architecture and Design for the FRATIS Dallas-Fort Worth DFW prototype system. The FRATIS prototype in DFW consisted of the following components: optimization algorithm, terminal wait time, route specific n...

A smartphone-based prototype system for incident/work zone management driven by crowd-sourced data.

DOT National Transportation Integrated Search

2015-02-01

This project develops a smartphone-based prototype system that supplements the 511 system to improve its dynamic traffic : routing service to state highway users under non-recurrent congestion. This system will save considerable time to provide cruci...

Design for effective development and prototyping of the HL-20

NASA Astrophysics Data System (ADS)

Urie, David M.; Floreck, Paul A.; McMorris, John A.; Elvin, John D.

1993-10-01

A feasibility study of the HL-20 personnel launch system (PLS) concept was conducted by a team which focused on creating a PLS design approach and an accelerated development plan consistent with the historical 'Skunk Works' approach to rapid prototyping. Technical design, manufacturing, system testing, and operations and support elements of the predefined baseline concept were evaluated. An initial phase program, featuring a concurrent system test during design and development, leading to the orbital flight of an unmanned HL-20 prototype on a Titan III launch system, was prescribed. A second-phase development and manufacturing plan leading to system operational status was also formulated. Baseline design feature modifications were made when necessary, without compromise to performance, to satisfy the prototype development plan. Technical design details and off-the-shelf hardware candidates were also identified for several subsystems, including the launch-system interface adapter/emergency escape system. The technical feasibility of the system and applicability of the Skunk Works approach to development of the HL-20/PLS were verified.

Securing Sensitive Flight and Engine Simulation Data Using Smart Card Technology

NASA Technical Reports Server (NTRS)

Blaser, Tammy M.

2003-01-01

NASA Glenn Research Center has developed a smart card prototype capable of encrypting and decrypting disk files required to run a distributed aerospace propulsion simulation. Triple Data Encryption Standard (3DES) encryption is used to secure the sensitive intellectual property on disk pre, during, and post simulation execution. The prototype operates as a secure system and maintains its authorized state by safely storing and permanently retaining the encryption keys only on the smart card. The prototype is capable of authenticating a single smart card user and includes pre simulation and post simulation tools for analysis and training purposes. The prototype's design is highly generic and can be used to protect any sensitive disk files with growth capability to urn multiple simulations. The NASA computer engineer developed the prototype on an interoperable programming environment to enable porting to other Numerical Propulsion System Simulation (NPSS) capable operating system environments.

Ultra-Wideband Time-Difference-of-Arrival High Resolution 3D Proximity Tracking System

NASA Technical Reports Server (NTRS)

Ni, Jianjun; Arndt, Dickey; Ngo, Phong; Phan, Chau; Dekome, Kent; Dusl, John

2010-01-01

This paper describes a research and development effort for a prototype ultra-wideband (UWB) tracking system that is currently under development at NASA Johnson Space Center (JSC). The system is being studied for use in tracking of lunar./Mars rovers and astronauts during early exploration missions when satellite navigation systems are not available. U IATB impulse radio (UWB-IR) technology is exploited in the design and implementation of the prototype location and tracking system. A three-dimensional (3D) proximity tracking prototype design using commercially available UWB products is proposed to implement the Time-Difference- Of-Arrival (TDOA) tracking methodology in this research effort. The TDOA tracking algorithm is utilized for location estimation in the prototype system, not only to exploit the precise time resolution possible with UWB signals, but also to eliminate the need for synchronization between the transmitter and the receiver. Simulations show that the TDOA algorithm can achieve the fine tracking resolution with low noise TDOA estimates for close-in tracking. Field tests demonstrated that this prototype UWB TDOA High Resolution 3D Proximity Tracking System is feasible for providing positioning-awareness information in a 3D space to a robotic control system. This 3D tracking system is developed for a robotic control system in a facility called "Moonyard" at Honeywell Defense & System in Arizona under a Space Act Agreement.

Smart roadside initiative : final report.

DOT National Transportation Integrated Search

2015-09-01

This is the Final Report for the Smart Roadside Initiative (SRI) prototype system deployment project. The SRI prototype was implemented at weigh stations in Grass Lake, Michigan and West Friendship, Maryland. The prototype was developed to integrate ...

Software Prototyping

PubMed Central

Del Fiol, Guilherme; Hanseler, Haley; Crouch, Barbara Insley; Cummins, Mollie R.

2016-01-01

Summary Background Health information exchange (HIE) between Poison Control Centers (PCCs) and Emergency Departments (EDs) could improve care of poisoned patients. However, PCC information systems are not designed to facilitate HIE with EDs; therefore, we are developing specialized software to support HIE within the normal workflow of the PCC using user-centered design and rapid prototyping. Objective To describe the design of an HIE dashboard and the refinement of user requirements through rapid prototyping. Methods Using previously elicited user requirements, we designed low-fidelity sketches of designs on paper with iterative refinement. Next, we designed an interactive high-fidelity prototype and conducted scenario-based usability tests with end users. Users were asked to think aloud while accomplishing tasks related to a case vignette. After testing, the users provided feedback and evaluated the prototype using the System Usability Scale (SUS). Results Survey results from three users provided useful feedback that was then incorporated into the design. After achieving a stable design, we used the prototype itself as the specification for development of the actual software. Benefits of prototyping included having 1) subject-matter experts heavily involved with the design; 2) flexibility to make rapid changes, 3) the ability to minimize software development efforts early in the design stage; 4) rapid finalization of requirements; 5) early visualization of designs; 6) and a powerful vehicle for communication of the design to the programmers. Challenges included 1) time and effort to develop the prototypes and case scenarios; 2) no simulation of system performance; 3) not having all proposed functionality available in the final product; and 4) missing needed data elements in the PCC information system. PMID:27081404

Use of geochemical signatures, including rare earth elements, in mosses and lichens to assess spatial integration and the influence of forest environment

NASA Astrophysics Data System (ADS)

Gandois, L.; Agnan, Y.; Leblond, S.; Séjalon-Delmas, N.; Le Roux, G.; Probst, A.

2014-10-01

In order to assess the influence of local environment and spatial integration of Trace Metals (TM) by biomonitors, Al, As, Cd, Cr, Cs, Cu, Fe, Mn, Ni, Pb, Sb, Sn, V and Zn and some rare earth element (REE) concentrations have been measured in lichens and mosses collected in three French forest sites located in three distinct mountainous areas, as well as in the local soil and bedrock, and in both bulk deposition (BD) and throughfall (TF). Similar enrichment factors (EF) were calculated using lichens and mosses and local bedrock for most elements, except for Cs, Mn, Ni, Pb, and Cu which were significantly (KW, p < 0.05) more enriched in mosses. Similar REE ratios were measured in soils, bedrock, lichens and mosses at each study sites, indicating a regional integration of atmospheric deposition by both biomonitors. Both TM signature and REE composition of mosses revealed that this biomonitor is highly influenced by throughfall composition, and reflect atmospheric deposition interaction with the forest canopy. This explained the higher enrichment measured in mosses for elements which concentration in deposition were influenced by the canopy, either due to leaching (Mn), direct uptake (Ni), or dry deposition dissolution (Pb, Cu, Cs).

Nanoparticle exposure biomonitoring: exposure/effect indicator development approaches

NASA Astrophysics Data System (ADS)

Marie-Desvergne, C.; Dubosson, M.; Lacombe, M.; Brun, V.; Mossuz, V.

2015-05-01

The use of engineered nanoparticles (NP) is more and more widespread in various industrial sectors. The inhalation route of exposure is a matter of concern (adverse effects of air pollution by ultrafine particles and asbestos). No NP biomonitoring recommendations or standards are available so far. The LBM laboratory is currently studying several approaches to develop bioindicators for occupational health applications. As regards exposure indicators, new tools are being implemented to assess potentially inhaled NP in non-invasive respiratory sampling (nasal sampling and exhaled breath condensates (EBC)). Diverse NP analytical characterization methods are used (ICP-MS, dynamic light scattering and electron microscopy coupled to energy-dispersive X-ray analysis). As regards effect indicators, a methodology has been developed to assess a range of 29 cytokines in EBCs (potential respiratory inflammation due to NP exposure). Secondly, collaboration between the LBM laboratory and the EDyp team has allowed the EBC proteome to be characterized by means of an LC-MS/MS process. These projects are expected to facilitate the development of individual NP exposure biomonitoring tools and the analysis of early potential impacts on health. Innovative techniques such as field-flow fractionation combined with ICP-MS and single particle-ICPMS are currently being explored. These tools are directly intended to assist occupational physicians in the identification of exposure situations.

Potential of Ranunculus acris L. for biomonitoring trace element contamination of riverbank soils: photosystem II activity and phenotypic responses for two soil series.

PubMed

Marchand, Lilian; Lamy, Pierre; Bert, Valerie; Quintela-Sabaris, Celestino; Mench, Michel

2016-02-01

Foliar ionome, photosystem II activity, and leaf growth parameters of Ranunculus acris L., a potential biomonitor of trace element (TE) contamination and phytoavailability, were assessed using two riverbank soil series. R. acris was cultivated on two potted soil series obtained by mixing a TE (Cd, Cu, Pb, and Zn)-contaminated technosol with either an uncontaminated sandy riverbank soil (A) or a silty clay one slightly contaminated by TE (B). Trace elements concentrations in the soil-pore water and the leaves, leaf dry weight (DW) yield, total leaf area (TLA), specific leaf area (SLA), and photosystem II activity were measured for both soil series after a 50-day growth period. As soil contamination increased, changes in soluble TE concentrations depended on soil texture. Increase in total soil TE did not affect the leaf DW yield, the TLA, the SLA, and the photosystem II activity of R. acris over the 50-day exposure. The foliar ionome did not reflect the total and soluble TE concentrations in both soil series. Foliar ionome of R. acris was only effective to biomonitor total and soluble soil Na concentrations in both soil series and total and soluble soil Mo concentrations in the soil series B.

Sensitive multiresidue method by HS-SPME/GC-MS for 10 volatile organic compounds in urine matrix: a new tool for biomonitoring studies on children.

PubMed

Antonucci, Arianna; Vitali, Matteo; Avino, Pasquale; Manigrasso, Maurizio; Protano, Carmela

2016-08-01

A HS-SPME method coupled with GC-MS analysis has been developed for simultaneously measuring the concentration of 10 volatile organic compounds (VOCs) (benzene, toluene, ethylbenzene, o-, m-, and p-xylene, methyl tert-butyl ether, ethyl tert-butyl ether, 2-methyl-2-butyl methyl ether, and diisopropyl ether) in urine matrix as a biomonitoring tool for populations at low levels of exposure to such VOCs. These compounds, potentially toxic for human health, are common contaminants of both outdoor and indoor air, as they are released by autovehicular traffic; some of them are also present in environmental tobacco smoke (ETS). Thus, the exposure to these pollutants cannot be neglected and should be assessed. The low limits of detection and quantification (LODs and LOQs <6.5 and 7.5 ng L(-1), respectively) and the high reproducibility (CVs <4 %) make the developed method suited for biomonitoring populations exposed at low levels such as children. Further, the method is cost-effective and low in time-consumption; therefore, it is useful for investigating large populations. It has been applied to children exposed to traffic pollution and/or ETS; the relevant results are reported, and the relevant implications are discussed.

Potential of Opuntia ficus-indica for air pollution biomonitoring: a lead isotopic study.

PubMed

El Hayek, Eliane; El Samrani, Antoine; Lartiges, Bruno; Kazpard, Veronique; Benoit, Mathieu; Munoz, Marguerite

2015-11-01

Opuntia ficus-indica (Ofi) is a long-domesticated cactus that is widespread throughout arid and semiarid regions. Ofi is grown for both its fruits and edible cladodes, which are flattened photosynthetic stems. Young cladodes develop from mother cladodes, thus forming series of cladodes of different ages. Therefore, successive cladodes may hold some potential for biomonitoring over several years the local atmospheric pollution. In this study, cladodes, roots, dust deposited onto the cladodes, and soil samples were collected in the vicinity of three heavily polluted sites, i.e., a fertilizer industry, the road side of a highway, and mine tailings. The lead content was analyzed using atomic absorption spectroscopy (AAS) and inductively coupled plasma-mass spectrometry (ICP-MS). Scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDX) was used to characterize the cladode surfaces and the nature of dust deposit, and the lead isotopes were analyzed to identify the origin of Pb. The results show that (i) Ofi readily bioaccumulates Pb, (ii) the lead isotopic composition of cladodes evidences a foliar pathway of lead into Ofi and identifies the relative contributions of local Pb sources, and (iii) an evolution of air quality is recorded with successive cladodes, which makes Ofi a potential biomonitor to be used in environmental and health studies.

Twenty-first century approaches to toxicity testing, biomonitoring, and risk assessment: perspectives from the global chemical industry.

PubMed

Phillips, Richard D; Bahadori, Tina; Barry, Brenda E; Bus, James S; Gant, Timothy W; Mostowy, Janet M; Smith, Claudia; Willuhn, Marc; Zimmer, Ulrike

2009-09-01

The International Council of Chemical Associations' Long-Range Research Initiative (ICCA-LRI) sponsored a workshop, titled Twenty-First Century Approaches to Toxicity Testing, Biomonitoring, and Risk Assessment, on 16 and 17 June 2008 in Amsterdam, The Netherlands. The workshop focused on interpretation of data from the new technologies for toxicity testing and biomonitoring, and on understanding the relevance of the new data for assessment of human health risks. Workshop participants articulated their concerns that scientific approaches for interpreting and understanding the emerging data in a biologically relevant context lag behind the rapid advancements in the new technologies. Research will be needed to mitigate these lags and to develop approaches for communicating the information, even in a context of uncertainty. A collaborative, coordinated, and sustained research effort is necessary to modernize risk assessment and to significantly reduce current reliance on animal testing. In essence, this workshop was a call to action to bring together the intellectual and financial resources necessary to harness the potential of these new technologies towards improved public health decision making. Without investment in the science of interpretation, it will be difficult to realize the potential that the advanced technologies offer to modernize toxicity testing, exposure science, and risk assessment.

Selecting Species Traits for Biomonitoring Applications in light of Phylogenetic Relationships among Lotic Insects

NASA Astrophysics Data System (ADS)

Poff, N.; Vieira, N. K.; Simmons, M. P.; Olden, J. D.; Kondratieff, B. C.; Finn, D. S.

2005-05-01

The use of species traits as indicators of environmental disturbance is being considered for biomonitoring programs globally. As such, methods to select relevant and informative traits for inclusion in biometrics need to be developed. In this research, we identified 20 traits of aquatic insects within six trait groups: morphology, mobility, life-history strategy, thermal tolerance, feeding guild and ecology (e.g., habitat preference). We constructed phylogenetic trees for 1) all lotic insect species of North America and 2) all Ephemeroptera, Plecoptera and Trichoptera species based on morphology- and molecular-based analyses and classifications. We then measured variability (i.e., plasticity) of the 20 traits and six trait groups across the two phylogenetic trees. Traits with higher degrees of plasticity indicated traits that were less phylogenetically constrained, and were considered informative for biomonitoring purposes. Thermal tolerance, rheophily, body size at maturity and feeding guild showed the highest plasticity across both phylogenetic trees. Two mobility traits, occurrence in drift and adult dispersal distance, showed moderate plasticity. By contrast, adult exiting ability, degree of attachment, adult lifespan and body shape showed low variability and were thus less informative. Plastic species traits that are less phylogenetically constrained may be most useful in detecting community change along environmental gradients.

Environmental epigenetics: A promising venue for developing next-generation pollution biomonitoring tools in marine invertebrates.

PubMed

Suarez-Ulloa, Victoria; Gonzalez-Romero, Rodrigo; Eirin-Lopez, Jose M

2015-09-15

Environmental epigenetics investigates the cause-effect relationships between specific environmental factors and the subsequent epigenetic modifications triggering adaptive responses in the cell. Given the dynamic and potentially reversible nature of the different types of epigenetic marks, environmental epigenetics constitutes a promising venue for developing fast and sensible biomonitoring programs. Indeed, several epigenetic biomarkers have been successfully developed and applied in traditional model organisms (e.g., human and mouse). Nevertheless, the lack of epigenetic knowledge in other ecologically and environmentally relevant organisms has hampered the application of these tools in a broader range of ecosystems, most notably in the marine environment. Fortunately, that scenario is now changing thanks to the growing availability of complete reference genome sequences along with the development of high-throughput DNA sequencing and bioinformatic methods. Altogether, these resources make the epigenetic study of marine organisms (and more specifically marine invertebrates) a reality. By building on this knowledge, the present work provides a timely perspective highlighting the extraordinary potential of environmental epigenetic analyses as a promising source of rapid and sensible tools for pollution biomonitoring, using marine invertebrates as sentinel organisms. This strategy represents an innovative, groundbreaking approach, improving the conservation and management of natural resources in the oceans. Copyright © 2015 Elsevier Ltd. All rights reserved.

DBS-platform for biomonitoring and toxicokinetics of toxicants: proof of concept using LC-MS/MS analysis of fipronil and its metabolites in blood

NASA Astrophysics Data System (ADS)

Raju, Kanumuri Siva Rama; Taneja, Isha; Rashid, Mamunur; Sonkar, Ashish Kumar; Wahajuddin, Muhammad; Singh, Sheelendra Pratap

2016-03-01

A simple, sensitive and high throughput LC-MS/MS method was developed and validated for quantification of fipronil, fipronil sulfone and fipronil desulfinyl in rat and human dried blood spots (DBS). DBS samples were prepared by spiking 10 μl blood on DMPK-C cards followed by drying at room temperature. The whole blood spots were then punched from the card and extracted using acetonitrile. The total chromatographic run time of the method was only 2 min. The lower limit of quantification of the method was 0.1 ng/ml for all the analytes. The method was successfully applied to determine fipronil desulfinyl in DBS samples obtained from its toxicokinetic study in rats following intravenous dose (1 mg/kg). In conclusion, the proposed DBS methodology has significant potential in toxicokinetics and biomonitoring studies of environmental toxicants. This microvolume DBS technique will be an ideal tool for biomonitoring studies, particularly in paediatric population. Small volume requirements, minimally invasive blood sampling method, easier storage and shipping procedure make DBS a suitable technique for such studies. Further, DBS technique contributes towards the principles of 3Rs resulting in significant reduction in the number of rodents used and refinement in sample collection for toxicokinetic studies.

Potential Uses of Biomonitoring Data: A Case Study Using the Organophosphorus Pesticides Chlorpyrifos and Malathion

PubMed Central

Barr, Dana B.; Angerer, Jürgen

2006-01-01

Background Organophosphorus pesticides such as chlorpyrifos and malathion are widely used insecticides. They do not bioaccumulate appreciably in humans and are rapidly metabolized and excreted in the urine. In nonoccupational settings, exposures to these pesticides are typically sporadic and short-lived because the pesticides tend to degrade in the environment over time; however, dietary exposures may be more chronic. Biologic monitoring has been widely used to assess exposures, susceptibility, and effects of chlorpyrifos and malathion; thus, the information base on these compounds is data rich. For biomonitoring of exposure, chlorpyrifos and malathion have been measured in blood, but most typically their urinary metabolites have been measured. For assessing early effects and susceptibility, cholinesterase and microsomal esterase activities, respectively, have been measured. Objectives Although many biologic monitoring data have been generated and published on these chemicals, their interpretation is not straightforward. For example, exposure to environmental degradates of chlorpyrifos and malathion may potentially increase f urinary metabolite levels, thus leading to overestimation of exposure. Also, the temporal nature of the exposures makes the evaluation of both exposure and effects difficult. We present an overview of the current biomonitoring and other relevant data available on exposure to chlorpyrifos and malathion and the use of these data in various environmental public health applications. PMID:17107865

Marine sponges with contrasting life histories can be complementary biomonitors of heavy metal pollution in coastal ecosystems.

PubMed

Batista, Daniela; Muricy, Guilherme; Rocha, Rafael Chávez; Miekeley, Norbert F

2014-05-01

In this study, we compared the usefulness of a long-living sponge (Hymeniacidon heliophila, Class Demospongiae) and a short-living one (Paraleucilla magna, Class Calcarea) as biomonitors of metallic pollution. The concentrations of 16 heavy metals were analyzed in both species along a gradient of decreasing pollution from the heavily polluted Guanabara Bay to the less impacted coastal islands in Rio de Janeiro, SE Brazil (SW Atlantic). The levels of most elements analyzed were higher in H. heliophila (Al, Co, Cr, Cu, Fe, Mn, Ni, Hg, Ni, and Sn) and P. magna (Ni, Cu, Mn, Al, Ti, Fe, Pb, Co, Cr, Zn, and V) collected from the heavily polluted bay when compared with the cleanest sites. Hymeniacidon heliophila accumulates 11 elements more efficiently than P. magna. This difference may be related to their skeleton composition, histological organization, symbiont bacteria and especially to their life cycle. Both species can be used as a biomonitors of metallic pollution, but while Hymeniacidon heliophila was more effective in concentrating most metals, Paraleucilla magna is more indicated to detect recent pollutant discharges due to its shorter life cycle. We suggest that the complementary use of species with contrasting life histories can be an effective monitoring strategy of heavy metals in coastal environments.

Ecological health monitoring of the Mekong River by using benthic algae in 2003-2004

NASA Astrophysics Data System (ADS)

Kunpradid, T.

2005-05-01

The monitoring of ecological health of the Mekong River by using benthic algae was carried out from 2003 - 2004. Thirty sampling sites along the Mekong River and its tributaries were selected in Laos, Thailand, Cambodia and Veitnam. In this investigation, the distribution of some species of benthic algae in different environments revealed that there was a significant relationship in the presence of them to the water quality, and these species could be used as a potential biomonitor of water quality in the Mekong River. One hundred and eighty six species of benthic diatoms and 46 species of macroalgae were found. Some dominant species of benthic algae could be used as biomonitors to assess water quality. Hydrodictyon recticulatum and Microspora floccosa and indicated clean-moderate water quality; Audouinella cylindrica, Cladophora glomerata, Achnanthes inflate and Cymbella turgidula indicated moderate water quality; Stigeoclonium flagelliforum, Aulacoseira granulata and Cymbella tumida indicated moderate-polluted water quality and Caloglossa leprieurii, Gomphonema parvulum and Nitzschia clausii indicated polluted water quality. The ecological health assessment of the Mekong River by using the species of benthic algae as biomonitors reveled that in the upstream and tributaries revealed moderate water quality. In contrast, some sites in the lower Mekong showed moderate-polluted to polluted water quality.

Using devitalized moss for active biomonitoring of water pollution.

PubMed

Debén, S; Fernández, J A; Carballeira, A; Aboal, J R

2016-03-01

This paper presents the results of an experiment carried out for the first time in situ to select a treatment to devitalize mosses for use in active biomonitoring of water pollution. Three devitalizing treatments for the aquatic moss Fontinalis antipyretica were tested (i.e. oven-drying at 100 °C, oven-drying with a 50-80-100 °C temperature ramp, and boiling in water), and the effects of these on loss of material during exposure of the transplants and on the accumulation of different heavy metals and metalloids were determined. The suitability of using devitalized samples of the terrestrial moss Sphagnum denticulatum to biomonitor aquatic environments was also tested. The structure of mosses was altered in different ways by the devitalizing treatments. Devitalization by boiling water led to significantly less loss of material (p < 0.01) than the oven-drying treatments. However, devitalization by oven-drying with a temperature ramp yielded more stable results in relation to both loss of material and accumulation of elements. With the aim of standardizing the moss bag technique, the use of F. antipyretica devitalized by oven-drying with a temperature ramp is recommended, rather than other devitalization treatments or use of S. denticulatum. Copyright © 2016 Elsevier Ltd. All rights reserved.

Development of prototype decision support systems for real-time freeway traffic routing. Volume I.

DOT National Transportation Integrated Search

1998-01-01

For a traffic management system (TMS) to improve traffic flow, TMS operators must develop effective routing strategies based on the data collected by the system. The purpose of this research was to build prototype decision support systems (DSS) for t...

C3I Rapid Prototype Investigation.

DTIC Science & Technology

1986-01-01

feasibility of applying rapid K prototyping techniques to Air Force C3 1 system developments . This report presents the technical progress during the...computer tunctions. The cost to use each in terms of hardware, software, analysis, and needed further developments was assessed. Prototyping approaches were...acquirer, and developer are the ". basis for problems in C3I system developments . These problems destabilize r-. the requirements determination process

Systems Prototyping with Fourth Generation Tools.

ERIC Educational Resources Information Center

Sholtys, Phyllis

1983-01-01

The development of information systems using an engineering approach that uses both traditional programing techniques and fourth generation software tools is described. Fourth generation applications tools are used to quickly develop a prototype system that is revised as the user clarifies requirements. (MLW)

Design data brochure: SIMS prototype system 2

NASA Technical Reports Server (NTRS)

1977-01-01

Information is provided on the design and performance of the IBM SIMS Prototype System 2, solar domestic hot water system, for single family residences. The document provides sufficient data to permit procurement, installation, operation, and maintenance by qualified architectural engineers or contractors.

A Clinical Information Display System

PubMed Central

Blum, Bruce J.; Lenhard, Raymond E.; Braine, Hayden; Kammer, Anne

1977-01-01

A clinical information display system has been implemented as part of a prototype Oncology Clinical Information System for the Johns Hopkins Oncology Center. The information system has been developed to support the management of patient therapy. Capabilities in the prototype include a patient data system, a patient abstract, a tumor registry, an appointment system, a census system, and a clinical information display system. This paper describes the clinical information display component of the prototype. It has the capability of supporting up to 10,000 patient records with online data entry and editing. At the present time, the system is being used only in the Oncology Center. There are plans, however, for trial use by other departments, and the system represents a tool with a potential for more general application.

An expert system prototype for aiding in the development of software functional requirements for NASA Goddard's command management system: A case study and lessons learned

NASA Technical Reports Server (NTRS)

Liebowitz, Jay

1986-01-01

At NASA Goddard, the role of the command management system (CMS) is to transform general requests for spacecraft opeerations into detailed operational plans to be uplinked to the spacecraft. The CMS is part of the NASA Data System which entails the downlink of science and engineering data from NASA near-earth satellites to the user, and the uplink of command and control data to the spacecraft. Presently, it takes one to three years, with meetings once or twice a week, to determine functional requirements for CMS software design. As an alternative approach to the present technique of developing CMS software functional requirements, an expert system prototype was developed to aid in this function. Specifically, the knowledge base was formulated through interactions with domain experts, and was then linked to an existing expert system application generator called 'Knowledge Engineering System (Version 1.3).' Knowledge base development focused on four major steps: (1) develop the problem-oriented attribute hierachy; (2) determine the knowledge management approach; (3) encode the knowledge base; and (4) validate, test, certify, and evaluate the knowledge base and the expert system prototype as a whole. Backcasting was accomplished for validating and testing the expert system prototype. Knowledge refinement, evaluation, and implementation procedures of the expert system prototype were then transacted.

The Effects of Sleep Deprivation on Dissociable Prototype Learning Systems

PubMed Central

Maddox, W. Todd; Glass, Brian D.; Zeithamova, Dagmar; Savarie, Zachary R.; Bowen, Christopher; Matthews, Michael D.; Schnyer, David M.

2011-01-01

Background: The cognitive neural underpinnings of prototype learning are becoming clear. Evidence points to 2 different neural systems, depending on the learning parameters. A/not-A (AN) prototype learning is mediated by posterior brain regions that are involved in early perceptual learning, whereas A/B (AB) is mediated by frontal and medial temporal lobe regions. Study Objectives: To investigate the effects of sleep deprivation on AN and AB prototype learning and to use established prototype models to provide insights into the cognitive-processing locus of sleep-deprivation deficits. Design: Participants performed an AN and an AB prototype learning task twice, separated by a 24-hour period, with or without sleep between testing sessions. Participants: Eighteen West Point cadets participated in the sleep-deprivation group, and 17 West Point cadets participated in a control group. Measurements and Results: Sleep deprivation led to an AN, but not an AB, performance deficit. Prototype model analyses indicated that the AN deficit was due to changes in attentional focus and a decrease in confidence that is reflected in an increased bias to respond non-A. Conclusions: The findings suggest that AN, but not AB, prototype learning is affected by sleep deprivation. Prototype model analyses support the notion that the effect of sleep deprivation on AN is consistent with lapses in attentional focus that are more detrimental to AN than to AB. This finding adds to a growing body of work that suggests that different performance changes associated with sleep deprivation can be attributed to a common mechanism of changes in simple attention and vigilance. Citation: Maddox WT; Glass BD; Zeithamova D; Savarie ZR; Bowen C; Matthews MD; Schnyer DM. The effects of sleep deprivation on dissociable prototype learning systems. SLEEP 2011;34(3):253-260. PMID:21358842

Metal uptake of Nerium oleander from aerial and underground organs and its use as a biomonitoring tool for airborne metallic pollution in cities.

PubMed

Vázquez, S; Martín, A; García, M; Español, C; Navarro, E

2016-04-01

The analysis of the airborne particulate matter-PM-incorporated to plant leaves may be informative of the air pollution in the surroundings, allowing their use as biomonitoring tools. Regarding metals, their accumulation in leaves can be the result of both atmospheric incorporation of metallic PM on aboveground plant organs and root uptake of soluble metals. In this study, the use of Nerium oleander leaves as a biomonitoring tool for metallic airborne pollution has been assessed. The metal uptake in N. oleander was assessed as follows: (a) for radicular uptake by irrigation with airborne metals as Pb, Cd, Cr, Ni, As, Ce and Zn (alone and in mixture) and (b) for direct leave exposure to urban PM. Plants showed a high resistance against the toxicity of metals under both single and multiple metal exposures. Except for Zn, the low values of translocation and bioaccumulation factors confirmed the excluder behaviour of N. oleander with respect to the metals provided by the irrigation. For metal uptake from airborne pollution, young plants grown under controlled conditions were deployed during 42 days in locations of the city of Zaragoza (700,000 h, NE Spain), differing in their level of traffic density. Samples of PM2.5 particles and the leaves of N. oleander were simultaneously collected weekly. High correlations in Pb concentrations were found between leaves and PM2.5; in a lesser extent, correlations were also found for Fe, Zn and Ti. Scanning electron microscopy showed the capture of airborne pollution particles in the large and abundant substomatal chambers of N. oleander leaves. Altogether, results indicate that N. Oleander, as a metal resistant plant by metal exclusion, is a suitable candidate as a biomonitoring tool for airborne metal pollution in urban areas.

New Exposure Biomarkers as Tools for Breast Cancer Epidemiology, Biomonitoring, and Prevention: A Systematic Approach Based on Animal Evidence

PubMed Central

Ackerman, Janet M.; Attfield, Kathleen R.; Brody, Julia Green

2014-01-01

Background: Exposure to chemicals that cause rodent mammary gland tumors is common, but few studies have evaluated potential breast cancer risks of these chemicals in humans. Objective: The goal of this review was to identify and bring together the needed tools to facilitate the measurement of biomarkers of exposure to potential breast carcinogens in breast cancer studies and biomonitoring. Methods: We conducted a structured literature search to identify measurement methods for exposure biomarkers for 102 chemicals that cause rodent mammary tumors. To evaluate concordance, we compared human and animal evidence for agents identified as plausibly linked to breast cancer in major reviews. To facilitate future application of exposure biomarkers, we compiled information about relevant cohort studies. Results: Exposure biomarkers have been developed for nearly three-quarters of these rodent mammary carcinogens. Analytical methods have been published for 73 of the chemicals. Some of the remaining chemicals could be measured using modified versions of existing methods for related chemicals. In humans, biomarkers of exposure have been measured for 62 chemicals, and for 45 in a nonoccupationally exposed population. The Centers for Disease Control and Prevention has measured 23 in the U.S. population. Seventy-five of the rodent mammary carcinogens fall into 17 groups, based on exposure potential, carcinogenicity, and structural similarity. Carcinogenicity in humans and rodents is generally consistent, although comparisons are limited because few agents have been studied in humans. We identified 44 cohort studies, with a total of > 3.5 million women enrolled, that have recorded breast cancer incidence and stored biological samples. Conclusions: Exposure measurement methods and cohort study resources are available to expand biomonitoring and epidemiology related to breast cancer etiology and prevention. Citation: Rudel RA, Ackerman JM, Attfield KR, Brody JG. 2014. New exposure biomarkers as tools for breast cancer epidemiology, biomonitoring, and prevention: a systematic approach based on animal evidence. Environ Health Perspect 122:881–895; http://dx.doi.org/10.1289/ehp.1307455 PMID:24818537

Human biomonitoring reference values for metals and trace elements in blood and urine derived from the Canadian Health Measures Survey 2007-2013.

PubMed

Saravanabhavan, Gurusankar; Werry, Kate; Walker, Mike; Haines, Douglas; Malowany, Morie; Khoury, Cheryl

2017-03-01

Human biomonitoring reference values are statistical estimates that indicate the upper margin of background exposure to a given chemical at a given time. Nationally representative human biomonitoring data on 176 chemicals, including several metals and trace elements, are available in Canada from 2007 to 2013 through the Canadian Health Measures Survey (CHMS). In this work, we used a systematic approach based on the reference interval concept proposed by the International Federation of Clinical Chemistry and Laboratory Medicine and the International Union of Pure and Applied Chemistry to derive reference values (RV 95 s) for metals and trace elements. These RV 95 s were derived for blood and urine matrices in the general Canadian population based on the latest biomonitoring data from the CHMS. Biomarkers were chosen based on specific selection criteria, including widespread detection in Canadians (≥66% detection rate). Reference populations were created for each biomarker by applying appropriate exclusion criteria. Age and sex were evaluated as possible partitioning criteria and separate RV 95 s were derived for the sub-populations in cases where partitioning was deemed necessary. The RV 95 s for metals and trace elements in blood ranged from 0.18μg/L for cadmium in young children aged 3-5 years to 7900μg/L for zinc in males aged 20-79 years. In the case of urinary biomarkers, the RV 95 s ranged from 0.17μg/L for antimony in the total population aged 3-79 years to 1400mg/L for fluoride in adults aged 20-79 years. These RV 95 s represent the first set of reference values for metals and trace elements in the general Canadian population. We compare the RV 95 s from other countries where available and discuss factors that could influence such comparisons. Copyright © 2016 The Authors. Published by Elsevier GmbH.. All rights reserved.

Biomonitoring levels of airborne metals around Urmia Lake using deciduous trees and evaluation of their tolerance for greenbelt development.

PubMed

Mohammadi, Amir; Mokhtari, Mehdi; Arani, Asghar Mosleh; Taghipour, Hassan; Hajizadeh, Yaghoub; Fallahzadeh, Hossein

2018-05-16

In the northwest of Iran, the dust of salty and toxic metals possibly caused due to drying Urmia Lake is threatening the health of surrounding communities. This study aimed to employ leaves of local deciduous trees for biomonitoring of toxic elements and to evaluate air pollution tolerance of the trees for greenbelt application. Sampling from leaves of four dominant tree species including Vitis vinifera, Juglans regia, Ulmus umbraculifera, and Popolus alba was carried out from gardens in two radial distances (5 and 10 km) around the Urmia Lake accounting for 16 sites. The concentration of metals in the leaves were extracted according to method USEPA method 3050B and measured by ICP AES technique. According to the levels of air pollution tolerance index (APTI), Popolus alba showed to be more sensitive to air pollution and can be applied for biomonitoring. The ranks of heavy metals and sodium concentrations in the leaves gained in the order of Na > Zn > Cu > Ni > Pb > As > Cd. The mean enrichment factor for the elements was calculated from 1 to 3, suggesting minor enrichment for them. As, Pb, and Na with similar spatial distribution were dominantly observed in northwest and center-east of the Urmia Lake. Potential ecological risk (PER) index showed a moderate risk in 6% of sampling zones, where Cd and As were identified as responsible pollutants. Principle component and correlation analysis between the elements depicted human sources such as industrial activity and road traffic for Cd, Cu, Ni, Pb, and Zn, whereas As and Na were most likely originated from the aerosols of Urmia Lake. Our findings showed that Popolus alba can be applied as a local biomonitor and Vitis vinifera with moderate tolerance can be used as a good air pollutant sink in greenbelt development around the drying Urmia Lake in the northwest of Iran.

Prototyping an institutional IAIMS/UMLS information environment for an academic medical center.

PubMed

Miller, P L; Paton, J A; Clyman, J I; Powsner, S M

1992-07-01

The paper describes a prototype information environment designed to link network-based information resources in an integrated fashion and thus enhance the information capabilities of an academic medical center. The prototype was implemented on a single Macintosh computer to permit exploration of the overall "information architecture" and to demonstrate the various desired capabilities prior to full-scale network-based implementation. At the heart of the prototype are two components: a diverse set of information resources available over an institutional computer network and an information sources map designed to assist users in finding and accessing information resources relevant to their needs. The paper describes these and other components of the prototype and presents a scenario illustrating its use. The prototype illustrates the link between the goals of two National Library of Medicine initiatives, the Integrated Academic Information Management System (IAIMS) and the Unified Medical Language System (UMLS).

SP-100 GES/NAT radiation shielding systems design and development testing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Disney, R.K.; Kulikowski, H.D.; McGinnis, C.A.

1991-01-10

Advanced Energy Systems (AES) of Westinghouse Electric Corporation is under subcontract to the General Electric Company to supply nuclear radiation shielding components for the SP-100 Ground Engineering System (GES) Nuclear Assembly Test to be conducted at Westinghouse Hanford Company at Richland, Washington. The radiation shielding components are integral to the Nuclear Assembly Test (NAT) assembly and include prototypic and non-prototypic radiation shielding components which provide prototypic test conditions for the SP-100 reactor subsystem and reactor control subsystem components during the GES/NAT operations. W-AES is designing three radiation shield components for the NAT assembly; a prototypic Generic Flight System (GFS) shield,more » the Lower Internal Facility Shield (LIFS), and the Upper Internal Facility Shield (UIFS). This paper describes the design approach and development testing to support the design, fabrication, and assembly of these three shield components for use within the vacuum vessel of the GES/NAT. The GES/NAT shields must be designed to operate in a high vacuum which simulates space operations. The GFS shield and LIFS must provide prototypic radiation/thermal environments and mechanical interfaces for reactor system components. The NAT shields, in combination with the test facility shielding, must provide adequate radiation attenuation for overall test operations. Special design considerations account for the ground test facility effects on the prototypic GFS shield. Validation of the GFS shield design and performance will be based on detailed Monte Carlo analyses and developmental testing of design features. Full scale prototype testing of the shield subsystems is not planned.« less

Preliminary design package for prototype solar heating system

NASA Technical Reports Server (NTRS)

1976-01-01

A preliminary design review on the development of a prototype solar heating system for single family dwellings is presented. The collector, storage, transport, control, and site data acquisition subsystems are described.

Prototype residential photovoltaic system: Evaluation results

NASA Astrophysics Data System (ADS)

Nichols, B. E.; Russell, M. C.

1982-09-01

Residential size photovoltaic power systems were discussed. Lessons learned from this experience, and performance summaries for the five prototype systems at the Northeast Residential Experiment Station and the system at the all electric Carlisle PV house are given. Results of evaluating five utility interactive residential size inverters also are reported.

Performance of the ERTS-1 DCS in a prototype volcano surveillance system

NASA Technical Reports Server (NTRS)

Ward, P. L.

1975-01-01

A prototype volcano surveillance system has been installed on 15 volcanoes in four states and four countries. The need for this system, the techniques used, the method of implementation, the major problems, the results, and the future seen for such a system are briefly reviewed.

SIMS prototype system 1: Design data brochure. [solar heating system

NASA Technical Reports Server (NTRS)

1978-01-01

A prototype solar heating and hot water system using air as the collector fluid and a pebble bed for heat storage was designed for installation into a single family dwelling. The system, subsystem, and installation requirements are described. System operation and performance are discussed, and procedures for sizing the system to a specific site are presented.

Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) Systems Integration Strategy

NASA Technical Reports Server (NTRS)

Gill, Tracy; Merbitz, Jerad; Kennedy, Kriss; Tri, Terry; Toups, Larry; Howe, A. Scott

2011-01-01

The Habitat Demonstration Unit (HDU) project team constructed an analog prototype lunar surface laboratory called the Pressurized Excursion Module (PEM). The prototype unit subsystems were integrated in a short amount of time, utilizing a rapid prototyping approach that brought together over 20 habitation-related technologies from a variety of NASA centers. This paper describes the system integration strategies and lessons learned, that allowed the PEM to be brought from paper design to working field prototype using a multi-center team. The system integration process was based on a rapid prototyping approach. Tailored design review and test and integration processes facilitated that approach. The use of collaboration tools including electronic tools as well as documentation enabled a geographically distributed team take a paper concept to an operational prototype in approximately one year. One of the major tools used in the integration strategy was a coordinated effort to accurately model all the subsystems using computer aided design (CAD), so conflicts were identified before physical components came together. A deliberate effort was made following the deployment of the HDU PEM for field operations to collect lessons learned to facilitate process improvement and inform the design of future flight or analog versions of habitat systems. Significant items within those lessons learned were limitations with the CAD integration approach and the impact of shell design on flexibility of placing systems within the HDU shell.

Integrating Clarus data in traffic signal system operation : a survivable real-time weather-responsive system.

DOT National Transportation Integrated Search

2011-07-11

This report presents a prototype of a secure, dependable, real-time weather-responsive traffic signal system. The prototype executes two tasks: 1) accesses weather information that provides near real-time atmospheric and pavement surface condition ob...

Autonomous Integrated Receive System (AIRS) requirements definition. Volume 4: Functional specification for the prototype Automated Integrated Receive System (AIRS)

NASA Technical Reports Server (NTRS)

Chie, C. M.

1984-01-01

The functional requirements for the performance, design, and testing for the prototype Automated Integrated Receive System (AIRS) to be demonstrated for the TDRSS S-Band Single Access Return Link are presented.

Prototyping Control and Data Acquisition for the ITER Neutral Beam Test Facility

NASA Astrophysics Data System (ADS)

Luchetta, Adriano; Manduchi, Gabriele; Taliercio, Cesare; Soppelsa, Anton; Paolucci, Francesco; Sartori, Filippo; Barbato, Paolo; Breda, Mauro; Capobianco, Roberto; Molon, Federico; Moressa, Modesto; Polato, Sandro; Simionato, Paola; Zampiva, Enrico

2013-10-01

The ITER Neutral Beam Test Facility will be the project's R&D facility for heating neutral beam injectors (HNB) for fusion research operating with H/D negative ions. Its mission is to develop technology to build the HNB prototype injector meeting the stringent HNB requirements (16.5 MW injection power, -1 MeV acceleration energy, 40 A ion current and one hour continuous operation). Two test-beds will be built in sequence in the facility: first SPIDER, the ion source test-bed, to optimize the negative ion source performance, second MITICA, the actual prototype injector, to optimize ion beam acceleration and neutralization. The SPIDER control and data acquisition system is under design. To validate the main architectural choices, a system prototype has been assembled and performance tests have been executed to assess the prototype's capability to meet the control and data acquisition system requirements. The prototype is based on open-source software frameworks running under Linux. EPICS is the slow control engine, MDSplus is the data handler and MARTe is the fast control manager. The prototype addresses low and high-frequency data acquisition, 10 kS/s and 10 MS/s respectively, camera image acquisition, data archiving, data streaming, data retrieval and visualization, real time fast control with 100 μs control cycle and supervisory control.

Software Development With Application Generators: The Naval Aviation Logistics Command Management Information System Case

DTIC Science & Technology

1992-09-01

Aviation Logistics Command Management Information System (NALCOMIS) prototyping development effort, the critical success factors required to implement prototyping with application generators in other areas of DoD.

Methods and systems for rapid prototyping of high density circuits

DOEpatents

Palmer, Jeremy A [Albuquerque, NM; Davis, Donald W [Albuquerque, NM; Chavez, Bart D [Albuquerque, NM; Gallegos, Phillip L [Albuquerque, NM; Wicker, Ryan B [El Paso, TX; Medina, Francisco R [El Paso, TX

2008-09-02

A preferred embodiment provides, for example, a system and method of integrating fluid media dispensing technology such as direct-write (DW) technologies with rapid prototyping (RP) technologies such as stereolithography (SL) to provide increased micro-fabrication and micro-stereolithography. A preferred embodiment of the present invention also provides, for example, a system and method for Rapid Prototyping High Density Circuit (RPHDC) manufacturing of solderless connectors and pilot devices with terminal geometries that are compatible with DW mechanisms and reduce contact resistance where the electrical system is encapsulated within structural members and manual electrical connections are eliminated in favor of automated DW traces. A preferred embodiment further provides, for example, a method of rapid prototyping comprising: fabricating a part layer using stereolithography and depositing thermally curable media onto the part layer using a fluid dispensing apparatus.

A Nano-Thin Film-Based Prototype QCM Sensor Array for Monitoring Human Breath and Respiratory Patterns.

PubMed

Selyanchyn, Roman; Wakamatsu, Shunichi; Hayashi, Kenshi; Lee, Seung-Woo

2015-07-31

Quartz crystal microbalance (QCM) sensor array was developed for multi-purpose human respiration assessment. The sensor system was designed to provide feedback for human respiration. Thorough optimization of measurement conditions: air flow, temperature in the QCM chamber, frequency measurement rate, and electrode position regarding to the gas flow-was performed. As shown, acquisition of respiratory parameters (rate and respiratory pattern) could be achieved even with a single electrode used in the system. The prototype system contains eight available QCM channels that can be potentially used for selective responses to certain breath chemicals. At present, the prototype machine is ready for the assessment of respiratory functions in larger populations in order to gain statistical validation. To the best of our knowledge, the developed prototype is the only respiratory assessment system based on surface modified QCM sensors.

Flight Telerobotic Servicer prototype simulator

NASA Astrophysics Data System (ADS)

Schein, Rob; Krauze, Linda; Hartley, Craig; Dickenson, Alan; Lavecchia, Tom; Working, Bob

A prototype simulator for the Flight Telerobotic Servicer (FTS) system is described for use in the design development of the FTS, emphasizing the hand controller and user interface. The simulator utilizes a graphics workstation based on rapid prototyping tools for systems analyses of the use of the user interface and the hand controller. Kinematic modeling, manipulator-control algorithms, and communications programs are contained in the software for the simulator. The hardwired FTS panels and operator interface for use on the STS Orbiter are represented graphically, and the simulated controls function as the final FTS system configuration does. The robotic arm moves based on the user hand-controller interface, and the joint angles and other data are given on the prototype of the user interface. This graphics simulation tool provides the means for familiarizing crewmembers with the FTS system operation, displays, and controls.

Manufacturing Laboratory for Next Generation Engineers

DTIC Science & Technology

2013-12-16

automated CNC machines, rapid prototype systems, robotic assembly systems, metrology , and non-traditional systems such as a waterjet cutter, EDM machine...CNC machines, rapid prototype systems, robotic assembly systems, metrology , and non-traditional systems such as a waterjet cutter, EDM machine, plasma...System Metrology and Quality Control Equipment - This area already had a CMM and other well known quality control instrumentation. It has been enhanced

Design of Inkjet-Printed RFID-Based Sensor on Paper: Single- and Dual-Tag Sensor Topologies.

PubMed

Kim, Sangkil; Georgiadis, Apostolos; Tentzeris, Manos M

2018-06-17

The detailed design considerations for the printed RFID-based sensor system is presented in this paper. Starting from material selection and metallization method, this paper discusses types of RFID-based sensors (single- & dual-tag sensor topologies), design procedures, and performance evaluation methods for the wireless sensor system. The electrical properties of the paper substrates (cellulose-based and synthetic papers) and the silver nano-particle-based conductive film are thoroughly characterized for RF applications up to 8 GHz. The reported technology could potentially set the foundation for truly “green”, low-cost, scalable wireless topologies for autonomous Internet-of-Things (IoT), bio-monitoring, and “smart skin” applications.

''Towards a High-Performance and Robust Implementation of MPI-IO on Top of GPFS''

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prost, J.P.; Tremann, R.; Blackwore, R.

2000-01-11

MPI-IO/GPFS is a prototype implementation of the I/O chapter of the Message Passing Interface (MPI) 2 standard. It uses the IBM General Parallel File System (GPFS), with prototyped extensions, as the underlying file system. this paper describes the features of this prototype which support its high performance and robustness. The use of hints at the file system level and at the MPI-IO level allows tailoring the use of the file system to the application needs. Error handling in collective operations provides robust error reporting and deadlock prevention in case of returning errors.

Hall Thruster Technology for NASA Science Missions

NASA Technical Reports Server (NTRS)

Manzella, David; Oh, David; Aadland, Randall

2005-01-01

The performance of a prototype Hall thruster designed for Discovery-class NASA science mission applications was evaluated at input powers ranging from 0.2 to 2.9 kilowatts. These data were used to construct a throttle profile for a projected Hall thruster system based on this prototype thruster. The suitability of such a Hall thruster system to perform robotic exploration missions was evaluated through the analysis of a near Earth asteroid sample return mission. This analysis demonstrated that a propulsion system based on the prototype Hall thruster offers mission benefits compared to a propulsion system based on an existing ion thruster.

A new database on contaminant exposure and effects in terrestrial vertebrates for natural resource managers

USGS Publications Warehouse

Rattner, B.A.; Pearson, J.L.; Garrett, L.J.; Erwin, R.M.; Walz, A.; Ottinger, M.A.; Barrett, H.R.

1997-01-01

The Biomonitoring of Environmental Status and Trends (BEST) program of the Department of the Interior is focused to identify and understand effects of contaminant stressors on biological resources under their stewardship. Despite the desire of many to continuously monitor the environmental health of our estuaries, much can be learned by summarizing existing temporal, geographic, and phylogenetic contaminant information. To this end, retrospective contaminant exposure and effects data for amphibians, reptiles, birds, and mammals residing within 30 km of Atlantic coast estuaries are being assembled through searches of published literature (e.g., Fisheries Review, Wildlife Review, BIOSIS Previews) and databases (e.g., US EPA Ecological Incident Information System; USGS Diagnostic and Epizootic Databases), and compilation of summary data from unpublished reports of government natural resource agencies, private conservation groups, and universities. These contaminant exposure and effect data for terrestrial vertebrates (CEE-TV) are being summarized using Borland dBASE in a 96- field format, including species, collection time and site coordinates, sample matrix, contaminant concentration, biomarker and bioindicator responses, and source of information (N>1500 records). This CEE-TV database has been imported into the ARC/INFO geographic information system (GIS), for purposes of examining geographic coverage and trends, and to identify critical data gaps. A preliminary risk assessment will be conducted to identify and characterize contaminants and other stressors potentially affecting terrestrial vertebrates that reside, migrate through or reproduce in these estuaries. Evaluations are underway, using specific measurement and assessment endpoints, to rank and prioritize estuarine ecosystems in which terrestrial vertebrates are potentially at risk for purposes of prediction and focusing future biomonitoring efforts.

Prototype solar heating and cooling systems including potable hot water

NASA Technical Reports Server (NTRS)

1978-01-01

Progress is reviewed in the development, delivery, and support of two prototype solar heating and cooling systems including potable hot water. The system consisted of the following subsystems: collector, auxiliary heating, potable hot water, storage, control, transport, and government-furnished site data acquisition.

Sustainability, synthetic chemicals, and human exposure.

PubMed

Podein, Rian J; Hernke, Michael T; Fortney, Luke W; Rakel, David P

2010-01-01

Public concerns regarding exposures to synthetic chemicals are increasing. Globally, there are increasing concentrations of many synthetic chemicals within the environment. The ubiquitous extent of some chemicals makes human exposure unavoidable. Biomonitoring has emerged as the optimal method for assessing exposures. The extent of human exposure and contamination occurs throughout the life cycle and is widespread. Although there is limited information on health risks for the majority of chemicals within our environment, and those identified with biomonitoring, many are known or suspected to cause human harm. Continued global and national unsustainable development regarding synthetic chemicals will increase the extent of environmental and human contamination unless precautionary action is implemented. Precautionary legislation may protect ecological and public health until societal sustainability is achieved.

Air Pollution Studies in Opole Region, Poland, using the Moss Biomonitoring and INAA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Korzekwa, S.; Pankratova, Yu. S.; Frontasyeva, M.V.

Biomonitoring of heavy metal atmospheric deposition with terrestrial moss is a well established technique for environmental studies. Moss samples of Hylocomium splendens and Pleurozium schreberi have been collected around the city of Opole. A total of 34 elements including heavy metals and rare earths have been determined by instrumental neutron activation analysis (INAA) using epithermal neutrons at the IBR-2 reactor of the Joint Institute for Nuclear Research. We observe pronounced contamination of the sampled area with pollutants such as As, Sb, V, Ni, Mo, etc. at levels similar to those in the neighboring industrial regions. These results evidences long-range atmosphericmore » transport of pollutants along with the influence of local pollution sources.« less

Active Moss Biomonitoring of Atmospheric Trace Element Deposition in Belgrade Urban Area using ENAA and AAS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anicic, M.; Tasic, M.; Tomasevic, M.

2007-11-26

Active biomonitoring of air quality in Belgrade, Serbia, was performed using the moss Sphagnum girgensohnii. Moss bags were exposed in parallel with and without irrigation respectively for four consecutive 3-month periods at three urban sites. Twenty-nine elements were determined in the exposed moss samples by ENAA and three (Cu, Cd, and Pb) by AAS. The relative accumulation factor (RAF) was greater than 1 for the majority of elements. Elements such as Cl, K, Rb and Cs, however, leached from the moss tissue during the exposure time. For all exposure periods, higher uptake in the irrigated moss bags was evident formore » Al, Cr, Fe, Cu, Zn, Sr, Pb, and Cd.« less

Illuminance and luminance distributions of a prototype ambient illumination system for Space Station Freedom

NASA Technical Reports Server (NTRS)

Mullican, R. C.; Hayes, B. C.

1991-01-01

Preliminary results of research conducted in the late 1970's indicate that perceptual qualities of an enclosure can be influenced by the distribution of illumination within the enclosure. Subjective impressions such as spaciousness, perceptual clarity, and relaxation or tenseness, among others, appear to be related to different combinations of surface luminance. A prototype indirect ambient illumination system was developed which will allow crew members to alter surface luminance distributions within an enclosed module, thus modifying perceptual cues to match crew preferences. A traditional lensed direct lighting system was compared to the prototype utilizing the full-scale mockup of Space Station Freedom developed by Marshall Space Flight Center. The direct lensed system was installed in the habitation module with the indirect prototype deployed in the U.S. laboratory module. Analysis centered on the illuminance and luminance distributions resultant from these systems and the implications of various luminaire spacing options. All test configurations were evaluated for compliance with NASA Standard 3000, Man-System Integration Standards.

Development of an Exhaled Breath Monitoring System with Semiconductive Gas Sensors, a Gas Condenser Unit, and Gas Chromatograph Columns

PubMed Central

Itoh, Toshio; Miwa, Toshio; Tsuruta, Akihiro; Akamatsu, Takafumi; Izu, Noriya; Shin, Woosuck; Park, Jangchul; Hida, Toyoaki; Eda, Takeshi; Setoguchi, Yasuhiro

2016-01-01

Various volatile organic compounds (VOCs) in breath exhaled by patients with lung cancer, healthy controls, and patients with lung cancer who underwent surgery for resection of cancer were analyzed by gas condenser-equipped gas chromatography-mass spectrometry (GC/MS) for development of an exhaled breath monitoring prototype system involving metal oxide gas sensors, a gas condenser, and gas chromatography columns. The gas condenser-GC/MS analysis identified concentrations of 56 VOCs in the breath exhaled by the test population of 136 volunteers (107 patients with lung cancer and 29 controls), and selected four target VOCs, nonanal, acetoin, acetic acid, and propanoic acid, for use with the condenser, GC, and sensor-type prototype system. The prototype system analyzed exhaled breath samples from 101 volunteers (74 patients with lung cancer and 27 controls). The prototype system exhibited a level of performance similar to that of the gas condenser-GC/MS system for breath analysis. PMID:27834896

Software Prototyping: A Case Report of Refining User Requirements for a Health Information Exchange Dashboard.

PubMed

Nelson, Scott D; Del Fiol, Guilherme; Hanseler, Haley; Crouch, Barbara Insley; Cummins, Mollie R

2016-01-01

Health information exchange (HIE) between Poison Control Centers (PCCs) and Emergency Departments (EDs) could improve care of poisoned patients. However, PCC information systems are not designed to facilitate HIE with EDs; therefore, we are developing specialized software to support HIE within the normal workflow of the PCC using user-centered design and rapid prototyping. To describe the design of an HIE dashboard and the refinement of user requirements through rapid prototyping. Using previously elicited user requirements, we designed low-fidelity sketches of designs on paper with iterative refinement. Next, we designed an interactive high-fidelity prototype and conducted scenario-based usability tests with end users. Users were asked to think aloud while accomplishing tasks related to a case vignette. After testing, the users provided feedback and evaluated the prototype using the System Usability Scale (SUS). Survey results from three users provided useful feedback that was then incorporated into the design. After achieving a stable design, we used the prototype itself as the specification for development of the actual software. Benefits of prototyping included having 1) subject-matter experts heavily involved with the design; 2) flexibility to make rapid changes, 3) the ability to minimize software development efforts early in the design stage; 4) rapid finalization of requirements; 5) early visualization of designs; 6) and a powerful vehicle for communication of the design to the programmers. Challenges included 1) time and effort to develop the prototypes and case scenarios; 2) no simulation of system performance; 3) not having all proposed functionality available in the final product; and 4) missing needed data elements in the PCC information system.

Design, Fabrication and Evaluation of Prototype Wayside Brake Inspection Sensors

DOT National Transportation Integrated Search

1980-06-01

Prototype Wayside instrumentation has been designed, developed, and tested that proves the feasibility of measuring braking system effectiveness on moving rail cars. The instrumentation system includes a specially designed short section of instrument...

Intelligent Network Flow Optimization (INFLO) prototype : Seattle small-scale demonstration report.

DOT National Transportation Integrated Search

2015-05-01

This report describes the performance and results of the INFLO Prototype Small-Scale Demonstration. The purpose of the Small-Scale Demonstration was to deploy the INFLO Prototype System to demonstrate its functionality and performance in an operation...

Installation package - SIMS prototype system 1A

NASA Technical Reports Server (NTRS)

1976-01-01

This report consists of details for the installation, operation and maintenance of a prototype heating and hot water system, designed for residential or light commercial applications. This system consists of the following subsystems: air type collectors, pebble bed thermal storage, air handling unit, air to water heat exchanger, hot water preheat tank, auxiliary energy, ducting system.

A prototype implementation of a network-level intrusion detection system. Technical report number CS91-11

DOE Office of Scientific and Technical Information (OSTI.GOV)

Heady, R.; Luger, G.F.; Maccabe, A.B.

1991-05-15

This paper presents the implementation of a prototype network level intrusion detection system. The prototype system monitors base level information in network packets (source, destination, packet size, time, and network protocol), learning the normal patterns and announcing anomalies as they occur. The goal of this research is to determine the applicability of current intrusion detection technology to the detection of network level intrusions. In particular, the authors are investigating the possibility of using this technology to detect and react to worm programs.

In-vehicle Information Systems Behavioral Model and Design Support: IVIS Demand Prototype Software User’s Manual

DOT National Transportation Integrated Search

2000-03-06

The purpose of this research was to develop a behavioral model and prototype computer program for evaluation of modern in-vehicle information systems (IVIS). These systems differ from earlier in-vehicle instruments and displays in that they may requi...

Prototype stop bar system evaluation at John F. Kennedy International Airport.

DOT National Transportation Integrated Search

1992-01-01

A prototype stop bar system was installed and evaluated at John F. Kennedy International Airport. The purpose of the year-long evaluation was to gain operational experience on the use of a stop bar system and how it could possibly impact the air traf...

Prototyping with Application Generators: Lessons Learned from the Naval Aviation Logistics Command Management Information System Case

DTIC Science & Technology

1992-10-01

Prototyping with Application Generators: Lessons Learned from the Naval Aviation Logistics Command Management Information System Case. This study... management information system to automate manual Naval aviation maintenance tasks-NALCOMIS. With the use of a fourth-generation programming language

In-Vehicle Information Systems Behavioral Model and Design Support: IVIS Demand Prototype Software User's Manual

DOT National Transportation Integrated Search

2000-03-06

The purpose of this research was to develop a behavioral model and prototype computer program for evaluation of modern in-vehicle information systems (IVIS). These systems differ from earlier in-vehicle instruments and displays in that they may requi...

Expert system development methodology and the transition from prototyping to operations: FIESTA, a case study

NASA Technical Reports Server (NTRS)

Happell, Nadine; Miksell, Steve; Carlisle, Candace

1989-01-01

A major barrier in taking expert systems from prototype to operational status involves instilling end user confidence in the operational system. The software of different life cycle models is examined and the advantages and disadvantages of each when applied to expert system development are explored. The Fault Isolation Expert System for Tracking and data relay satellite system Applications (FIESTA) is presented as a case study of development of an expert system. The end user confidence necessary for operational use of this system is accentuated by the fact that it will handle real-time data in a secure environment, allowing little tolerance for errors. How FIESTA is dealing with transition problems as it moves from an off-line standalone prototype to an on-line real-time system is discussed.

Expert system development methodology and the transition from prototyping to operations - Fiesta, a case study

NASA Technical Reports Server (NTRS)

Happell, Nadine; Miksell, Steve; Carlisle, Candace

1989-01-01

A major barrier in taking expert systems from prototype to operational status involves instilling end user confidence in the operational system. The software of different life cycle models is examined and the advantages and disadvantages of each when applied to expert system development are explored. The Fault Isolation Expert System for Tracking and data relay satellite system Applications (FIESTA) is presented as a case study of development of an expert system. The end user confidence necessary for operational use of this system is accentuated by the fact that it will handle real-time data in a secure environment, allowing little tolerance for errors. How FIESTA is dealing with transition problems as it moves from an off-line standalone prototype to an on-line real-time system is discussed.

A prototype table-top inverse-geometry volumetric CT system.

PubMed

Schmidt, Taly Gilat; Star-Lack, Josh; Bennett, N Robert; Mazin, Samuel R; Solomon, Edward G; Fahrig, Rebecca; Pelc, Norbert J

2006-06-01

A table-top volumetric CT system has been implemented that is able to image a 5-cm-thick volume in one circular scan with no cone-beam artifacts. The prototype inverse-geometry CT (IGCT) scanner consists of a large-area, scanned x-ray source and a detector array that is smaller in the transverse direction. The IGCT geometry provides sufficient volumetric sampling because the source and detector have the same axial, or slice direction, extent. This paper describes the implementation of the table-top IGCT scanner, which is based on the NexRay Scanning-Beam Digital X-ray system (NexRay, Inc., Los Gatos, CA) and an investigation of the system performance. The alignment and flat-field calibration procedures are described, along with a summary of the reconstruction algorithm. The resolution and noise performance of the prototype IGCT system are studied through experiments and further supported by analytical predictions and simulations. To study the presence of cone-beam artifacts, a "Defrise" phantom was scanned on both the prototype IGCT scanner and a micro CT system with a +/-5 cone angle for a 4.5-cm volume thickness. Images of inner ear specimens are presented and compared to those from clinical CT systems. Results showed that the prototype IGCT system has a 0.25-mm isotropic resolution and that noise comparable to that from a clinical scanner with equivalent spatial resolution is achievable. The measured MTF and noise values agreed reasonably well with theoretical predictions and computer simulations. The IGCT system was able to faithfully reconstruct the laminated pattern of the Defrise phantom while the micro CT system suffered severe cone-beam artifacts for the same object. The inner ear acquisition verified that the IGCT system can image a complex anatomical object, and the resulting images exhibited more high-resolution details than the clinical CT acquisition. Overall, the successful implementation of the prototype system supports the IGCT concept for single-rotation volumetric scanning free from cone-beam artifacts.

System design document for the INFLO prototype.

DOT National Transportation Integrated Search

2014-03-01

This report documents the high level System Design Document (SDD) for the prototype development and demonstration of the Intelligent Network Flow Optimization (INFLO) application bundle, with a focus on the Speed Harmonization (SPD-HARM) and Queue Wa...

Web Mining for Web Image Retrieval.

ERIC Educational Resources Information Center

Chen, Zheng; Wenyin, Liu; Zhang, Feng; Li, Mingjing; Zhang, Hongjiang

2001-01-01

Presents a prototype system for image retrieval from the Internet using Web mining. Discusses the architecture of the Web image retrieval prototype; document space modeling; user log mining; and image retrieval experiments to evaluate the proposed system. (AEF)

Modeling and prototyping of biometric systems using dataflow programming

NASA Astrophysics Data System (ADS)

Minakova, N.; Petrov, I.

2018-01-01

The development of biometric systems is one of the labor-intensive processes. Therefore, the creation and analysis of approaches and techniques is an urgent task at present. This article presents a technique of modeling and prototyping biometric systems based on dataflow programming. The technique includes three main stages: the development of functional blocks, the creation of a dataflow graph and the generation of a prototype. A specially developed software modeling environment that implements this technique is described. As an example of the use of this technique, an example of the implementation of the iris localization subsystem is demonstrated. A variant of modification of dataflow programming is suggested to solve the problem related to the undefined order of block activation. The main advantage of the presented technique is the ability to visually display and design the model of the biometric system, the rapid creation of a working prototype and the reuse of the previously developed functional blocks.

A second generation 50 Mbps VLSI level zero processing system prototype

NASA Technical Reports Server (NTRS)

Harris, Jonathan C.; Shi, Jeff; Speciale, Nick; Bennett, Toby

1994-01-01

Level Zero Processing (LZP) generally refers to telemetry data processing functions performed at ground facilities to remove all communication artifacts from instrument data. These functions typically include frame synchronization, error detection and correction, packet reassembly and sorting, playback reversal, merging, time-ordering, overlap deletion, and production of annotated data sets. The Data Systems Technologies Division (DSTD) at Goddard Space Flight Center (GSFC) has been developing high-performance Very Large Scale Integration Level Zero Processing Systems (VLSI LZPS) since 1989. The first VLSI LZPS prototype demonstrated 20 Megabits per second (Mbp's) capability in 1992. With a new generation of high-density Application-specific Integrated Circuits (ASIC) and a Mass Storage System (MSS) based on the High-performance Parallel Peripheral Interface (HiPPI), a second prototype has been built that achieves full 50 Mbp's performance. This paper describes the second generation LZPS prototype based upon VLSI technologies.

A Nano-Thin Film-Based Prototype QCM Sensor Array for Monitoring Human Breath and Respiratory Patterns

PubMed Central

Selyanchyn, Roman; Wakamatsu, Shunichi; Hayashi, Kenshi; Lee, Seung-Woo

2015-01-01

Quartz crystal microbalance (QCM) sensor array was developed for multi-purpose human respiration assessment. The sensor system was designed to provide feedback for human respiration. Thorough optimization of measurement conditions: air flow, temperature in the QCM chamber, frequency measurement rate, and electrode position regarding to the gas flow—was performed. As shown, acquisition of respiratory parameters (rate and respiratory pattern) could be achieved even with a single electrode used in the system. The prototype system contains eight available QCM channels that can be potentially used for selective responses to certain breath chemicals. At present, the prototype machine is ready for the assessment of respiratory functions in larger populations in order to gain statistical validation. To the best of our knowledge, the developed prototype is the only respiratory assessment system based on surface modified QCM sensors. PMID:26263994

Prototype of NASA's Global Precipitation Measurement Mission Ground Validation System

NASA Technical Reports Server (NTRS)

Schwaller, M. R.; Morris, K. R.; Petersen, W. A.

2007-01-01

NASA is developing a Ground Validation System (GVS) as one of its contributions to the Global Precipitation Mission (GPM). The GPM GVS provides an independent means for evaluation, diagnosis, and ultimately improvement of GPM spaceborne measurements and precipitation products. NASA's GPM GVS consists of three elements: field campaigns/physical validation, direct network validation, and modeling and simulation. The GVS prototype of direct network validation compares Tropical Rainfall Measuring Mission (TRMM) satellite-borne radar data to similar measurements from the U.S. national network of operational weather radars. A prototype field campaign has also been conducted; modeling and simulation prototypes are under consideration.

Prototype development of a Geostationary Synthetic Thinned Aperture Radiometer, GeoSTAR

NASA Technical Reports Server (NTRS)

Tanner, A. B.; Wilson, W. J.; Kangaslahti, P. P.; Lambrigsten, B. H.; Dinardo, S. J.; Piepmeier, J. R.; Ruf, C. S.; Rogacki, S.; Gross, S. M.; Musko, S.

2004-01-01

Preliminary details of a 2-D synthetic aperture radiometer prototype operating from 50 to 55 GHz will be presented. The laboratory prototype is being developed to demonstrate the technologies and system design needed to do millimeter-wave atmospheric soundings with high spatial resolution from Geostationary orbit. The concept is to deploy a large thinned aperture Y-array on a geostationary satellite, and to use aperture synthesis to obtain images of the Earth without the need for a large mechanically scanned antenna. The laboratory prototype consists of a Y-array of 24 horn antennas, MMIC receivers, and a digital cross-correlation sub-system.

156 Mbps Ultrahigh-Speed Wireless LAN Prototype in the 38 GHz Band

NASA Astrophysics Data System (ADS)

Wu, Gang; Inoue, Masugi; Murakami, Homare; Hase, Yoshihiro

2001-12-01

This paper describes a 156 Mbps ultrahigh-speed wireless LAN operating in the 38 GHz millimeter (mm)-wave band. The system is a third prototype developed at the Communications Research Laboratory since 1998. Compared with the previous prototypes, the system is faster (156 Mbps) and smaller (volume of radio transceiver less than 1000 cc), it has a larger service area (two overlapping basic service sets), and a longer transmission distance (the protocol can support a distance of more than two hundred meters). The development is focused on the physical layer and the data link control layer, and thus a GMSK-based mm-wave transceiver and an enhanced RS-ISMA (reservation-based slotted idle signal multiple access) protocol are key development components. This paper describes the prototype system's design, configuration, and implementation.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Humble, Travis S; Sadlier, Ronald J

Quantum communication systems harness modern physics through state-of-the-art optical engineering to provide revolutionary capabilities. An important concern for quantum communication engineering is designing and prototyping these systems to prototype proposed capabilities. We apply the paradigm of software-defined communica- tion for engineering quantum communication systems to facilitate rapid prototyping and prototype comparisons. We detail how to decompose quantum communication terminals into functional layers defining hardware, software, and middleware concerns, and we describe how each layer behaves. Using the super-dense coding protocol as a test case, we describe implementations of both the transmitter and receiver, and we present results from numerical simulationsmore » of the behavior. We find that while the theoretical benefits of super dense coding are maintained, there is a classical overhead associated with the full implementation.« less

Mercury and Your Health

MedlinePlus

... the Risk of Exposure to Mercury Learn About Mercury What is Mercury What is Metallic mercury? Toxicological Profile ToxFAQs Mercury Resources CDC’s National Biomonitoring Program Factsheet on Mercury ...

A demonstration of expert systems applications in transportation engineering : volume II, TRANZ, a prototype expert system for traffic control in highway work zones.

DOT National Transportation Integrated Search

1988-01-01

The development of a prototype knowledge-based expert system (KBES) for selecting appropriate traffic control strategies and management techniques around highway work zones was initiated. This process was encompassed by the steps that formulate the p...

Classroom Evaluation of a Rapid Prototyping System.

ERIC Educational Resources Information Center

Tennyson, Stephen A.; Krueger, Thomas J.

2001-01-01

Introduces rapid prototyping which creates virtual models through a variety of automated material additive processes. Relates experiences using JP System 5 in freshman and sophomore engineering design graphics courses. Analyzes strengths and limitations of the JP System 5 and discusses how to use it effectively. (Contains 15 references.)…

Development of the prototype data management system of the solar H-alpha full disk observation

NASA Astrophysics Data System (ADS)

Wei, Ka-Ning; Zhao, Shi-Qing; Li, Qiong-Ying; Chen, Dong

2004-06-01

The Solar Chromospheric Telescope in Yunnan Observatory generates about 2G bytes fits format data per day. Huge amounts of data will bring inconvenience for people to use. Hence, data searching and sharing are important at present. Data searching, on-line browsing, remote accesses and download are developed with a prototype data management system of the solar H-alpha full disk observation, and improved by the working flow technology. Based on Windows XP operating system and MySQL data management system, a prototype system of browse/server model is developed by JAVA and JSP. Data compression, searching, browsing, deletion need authority and download in real-time have been achieved.

Description and field test of an in situ coliform monitoring system

NASA Technical Reports Server (NTRS)

Grana, D. C.; Wilkins, J. R.

1979-01-01

A prototype in situ system for monitoring the levels of fecal coliforms in shallow water bodies was developed and evaluated. This system was based on the known relationship between the concentration of the coliform bacteria and the amount of hydrogen they produce during growth in a complex organic media. The prototype system consists of a sampler platform, which sits on the bottom; a surface buoy, which transmits sampler-generated data; and a shore station, which receives, displays the data, and controls the sampler. The concept of remote monitoring of fecal coliform concentrations by utilizing a system based on the electrochemical method was verified during the evaluation of the prototype.

Design and Prototyping of Hard Real Time Systems

DTIC Science & Technology

1990-06-01

AD’-A254 097 DESIGNadPROTOTYPING (9 lliIlhIIIIIl/IIIIIliIliiiiliii OF HARD REAL TIME SYSTEMS Electronics Research Laboratory - - __University of...e ...*...*... . e * ... , .. * 311 2.9 Design of Real - Time Systems with Application to Robotics...Right now we are looking into possible techniques and the implications on the system architecture and partitioning. 2.9. Design of Real - Time Systems with

Complex of technologies and prototype systems for eco-friendly shutdown of the power-generating, process, capacitive, and transport equipment

NASA Astrophysics Data System (ADS)

Smorodin, A. I.; Red'kin, V. V.; Frolov, Y. D.; Korobkov, A. A.; Kemaev, O. V.; Kulik, M. V.; Shabalin, O. V.

2015-07-01

A set of technologies and prototype systems for eco-friendly shutdown of the power-generating, process, capacitive, and transport equipment is offered. The following technologies are regarded as core technologies for the complex: cryogenic technology nitrogen for displacement of hydrogen from the cooling circuit of turbine generators, cryo blasting of the power units by dioxide granules, preservation of the shutdown power units by dehydrated air, and dismantling and severing of equipment and structural materials of power units. Four prototype systems for eco-friendly shutdown of the power units may be built on the basis of selected technologies: Multimode nitrogen cryogenic system with four subsystems, cryo blasting system with CO2 granules for thermal-mechanical and electrical equipment of power units, and compressionless air-drainage systems for drying and storage of the shutdown power units and cryo-gas system for general severing of the steam-turbine power units. Results of the research and pilot and demonstration tests of the operational units of the considered technological systems allow applying the proposed technologies and systems in the prototype systems for shutdown of the power-generating, process, capacitive, and transport equipment.

Hybrid neuro-fuzzy approach for automatic vehicle license plate recognition

NASA Astrophysics Data System (ADS)

Lee, Hsi-Chieh; Jong, Chung-Shi

1998-03-01

Most currently available vehicle identification systems use techniques such as R.F., microwave, or infrared to help identifying the vehicle. Transponders are usually installed in the vehicle in order to transmit the corresponding information to the sensory system. It is considered expensive to install a transponder in each vehicle and the malfunction of the transponder will result in the failure of the vehicle identification system. In this study, novel hybrid approach is proposed for automatic vehicle license plate recognition. A system prototype is built which can be used independently or cooperating with current vehicle identification system in identifying a vehicle. The prototype consists of four major modules including the module for license plate region identification, the module for character extraction from the license plate, the module for character recognition, and the module for the SimNet neuro-fuzzy system. To test the performance of the proposed system, three hundred and eighty vehicle image samples are taken by a digital camera. The license plate recognition success rate of the prototype is approximately 91% while the character recognition success rate of the prototype is approximately 97%.

Anti-Idling Battery for Truck Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Keith Kelly

2011-09-30

In accordance to the Assistance Agreement DE-EE0001036, the objective of this project was to develop an advanced high voltage lithium-ion battery for use in an all-electric HVAC system for Class-7-8 heavy duty trucks. This system will help heavy duty truck drivers meet the tough new anti-idling laws being implemented by over 23 states. Quallion will be partnering with a major OEM supplier of HVAC systems to develop this system. The major OEM supplier will provide Quallion the necessary interface requirements and HVAC hardware to ensure successful testing of the all-electric system. At the end of the program, Quallion will delivermore » test data on three (3) batteries as well as test data for the prototype HVAC system. The objectives of the program are: (1) Battery Development - Objective 1 - Define battery and electronics specifications in preparation for building the prototype module. (Completed - summary included in report) and Objective 2 - Establish a functional prototype battery and characterize three batteries in-house. (Completed - photos and data included in report); (2) HVAC Development - Objective 1 - Collaborate with manufacturers to define HVAC components, layout, and electronics in preparation for establishing the prototype system. (Completed - photos and data included in report) and Objective 2 - Acquire components for three functional prototypes for use by Quallion. (Completed - photos and data included in report).« less

The load shedding advisor: An example of a crisis-response expert system

NASA Technical Reports Server (NTRS)

Bollinger, Terry B.; Lightner, Eric; Laverty, John; Ambrose, Edward

1987-01-01

A Prolog-based prototype expert system is described that was implemented by the Network Operations Branch of the NASA Goddard Space Flight Center. The purpose of the prototype was to test whether a small, inexpensive computer system could be used to host a load shedding advisor, a system which would monitor major physical environment parameters in a computer facility, then recommend appropriate operator reponses whenever a serious condition was detected. The resulting prototype performed significantly to efficiency gains achieved by replacing a purely rule-based design methodology with a hybrid approach that combined procedural, entity-relationship, and rule-based methods.

The resource envelope as a basis for space station management system scheduling

NASA Technical Reports Server (NTRS)

Bush, Joy; Critchfield, Anna

1987-01-01

The Platform Management System (PMS) Resource Envelope Scheduling System (PRESS) expert system prototype developed for space station scheduling is described. The purpose of developing the prototype was too investigate the resource envelope concept in a practical scheduling application, using a commercially available expert system shell. PRESS is being developed on an IBM PC/AT using Teknowledge, Inc.'s M.1 expert system shell.

Sims Prototype System 2 test results: Engineering analysis

NASA Technical Reports Server (NTRS)

1978-01-01

The testing, problems encountered, and the results and conclusions obtained from tests performed on the IBM Prototype System, 2, solar hot water system, at the Marshall Space Flight Center Solar Test Facility was described. System 2 is a liquid, non draining solar energy system for supplying domestic hot water to single residences. The system consists of collectors, storage tank, heat exchanger, pumps and associated plumbing and controls.

Biomonitoring and risk assessment on earth and during exploratory missions using AquaHab ®

NASA Astrophysics Data System (ADS)

Slenzka, K.; Dünne, M.; Jastorff, B.

2008-12-01

Bioregenerative closed ecological life support systems (CELSS) will be necessary in the exploration context revitalizing atmosphere, waste water and producing food for the human CELSS mates. During these long-term space travels and stays far away from Earth in an hostile environment as well as far for example from any hospital and surgery potential, it will be necessary to know much more about chemical and drug contamination in the special sense and by human's themselves in detail. Additionally, there is a strong need on Earth for more relevant standardized test systems including aquatic ones for the prospective risk assessment of chemicals and drugs in general on a laboratory scale. Current standardized test systems are mono species tests, and thus do not represent system aspects and have reduced environmental relevance. The experience gained during the last years in our research group lead to the development of a self-sustaining closed aquatic habitat/facility, called AquaHab ® which can serve regarding space exploration and Earth application. The AquaHab ® module can be the home of several fish species, snails, plants, amphipods and bacteria. The possibility to use different effect endpoints with certain beneficial characteristics is the basis for the application of AquaHab ® in different fields. Influence of drugs and chemicals can be tested on several trophic levels and ecosystem levels; guaranteeing a high relevance for aquatic systems in the real environment. Analyses of effect parameters of different complexity (e.g. general biological and water chemical parameters, activity of biotransforming enzymes) result in broad spectra of sensitivity. Combined with residual analyses (including all metabolites), this leads to an extended prospective risk assessment of a chemical on Earth and in a closed Life Support System. The possibility to measure also sensitive "online" parameters (e.g. behavior, respiration/photosynthetic activity) enables a quick and sensitive effect analysis of water contaminants in respective environments. AquaHab ® is currently under development to an early warning biomonitoring system using genetically modified fish and green algae. The implementation of biosensors/biochip in addition is also discussed.

Deployment Ready Airway Management System (DRAMS)

DTIC Science & Technology

2013-10-24

have been developed along with rapid prototypes. The results have been excellent and DMLS Alpha one and two prototypes have been developed resulting...Contact Model Quarterly  Report               10/25/2013 DMLS FlexBlade Reusable Module B-1 Prototype

User engineering: A new look at system engineering

NASA Technical Reports Server (NTRS)

Mclaughlin, Larry L.

1987-01-01

User Engineering is a new System Engineering perspective responsible for defining and maintaining the user view of the system. Its elements are a process to guide the project and customer, a multidisciplinary team including hard and soft sciences, rapid prototyping tools to build user interfaces quickly and modify them frequently at low cost, and a prototyping center for involving users and designers in an iterative way. The main consideration is reducing the risk that the end user will not or cannot effectively use the system. The process begins with user analysis to produce cognitive and work style models, and task analysis to produce user work functions and scenarios. These become major drivers of the human computer interface design which is presented and reviewed as an interactive prototype by users. Feedback is rapid and productive, and user effectiveness can be measured and observed before the system is built and fielded. Requirements are derived via the prototype and baselined early to serve as an input to the architecture and software design.

Public webinar: Wildland Fire Sensors Challenge

EPA Science Inventory

This multi-agency challenge seeks a field-ready prototype system capable of measuring constituents of smoke, including particulates, carbon monoxide, ozone, and carbon dioxide, over the wide range of levels expected during wildland fires. The prototype system should be accurate, ...

Evaluation of Prototype Automatic Truck Rollover Warning Systems

DOT National Transportation Integrated Search

1998-01-01

Three operating prototype Automatic Truck Rollover Warning Systems (ATRWS) installed on the Capital Beltway in Maryland and Virginia were evaluated for 3 years. The general objectives of this evaluation were to assess how the ATRWS performed and to d...

Analysis of Hydraulic Servo Equations for WRDRF Prototype Control System : Volume I

DOT National Transportation Integrated Search

1971-10-01

A set of dynamic performance equations derived by Wylie Labs., Huntsville, Alabama, were independently rederived and checked. These equations describe the perfromance of the prototype electro hydraulic servo actuator system selected by Wylie as repre...

UAS C2 Radio System - Final Phase 1 Development and Testing

NASA Technical Reports Server (NTRS)

Kerczewski, Robert; Shalkhauser, Kurt

2017-01-01

Phase 1 of the Command and Control Communications (C2) Subproject of NASA's UAS Integration in the National Airspace System Project included the development and testing of prototype C2 radio systems. This information paper provides an overview of the functionality and testing of the fifth and final Phase 1 generation of the prototype radio system.

Building a generalized distributed system model

NASA Technical Reports Server (NTRS)

Mukkamala, R.

1992-01-01

The key elements in the second year (1991-92) of our project are: (1) implementation of the distributed system prototype; (2) successful passing of the candidacy examination and a PhD proposal acceptance by the funded student; (3) design of storage efficient schemes for replicated distributed systems; and (4) modeling of gracefully degrading reliable computing systems. In the third year of the project (1992-93), we propose to: (1) complete the testing of the prototype; (2) enhance the functionality of the modules by enabling the experimentation with more complex protocols; (3) use the prototype to verify the theoretically predicted performance of locking protocols, etc.; and (4) work on issues related to real-time distributed systems. This should result in efficient protocols for these systems.

Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring

PubMed Central

Woutersen, Marjolijn; van der Gaag, Bram; Abrafi Boakye, Afua; Mink, Jan; Marks, Robert S.; Wagenvoort, Arco J.; Ketelaars, Henk A. M.; Brouwer, Bram; Heringa, Minne B.

2017-01-01

Surface water used for drinking water production is frequently monitored in The Netherlands using whole organism biomonitors, with for example Daphnia magna or Dreissena mussels, which respond to changes in the water quality. However, not all human-relevant toxic compounds can be detected by these biomonitors. Therefore, a new on-line biosensor has been developed, containing immobilized genetically modified bacteria, which respond to genotoxicity in the water by emitting luminescence. The performance of this sensor was tested under laboratory conditions, as well as under field conditions at a monitoring station along the river Meuse in The Netherlands. The sensor was robust and easy to clean, with inert materials, temperature control and nutrient feed for the reporter organisms. The bacteria were immobilized in sol-gel on either an optical fiber or a glass slide and then continuously exposed to water. Since the glass slide was more sensitive and robust, only this setup was used in the field. The sensor responded to spikes of genotoxic compounds in the water with a minimal detectable concentration of 0.01 mg/L mitomycin C in the laboratory and 0.1 mg/L mitomycin C in the field. With further optimization, which should include a reduction in daily maintenance, the sensor has the potential to become a useful addition to the currently available biomonitors. PMID:29165334

Biodiversity of nematode assemblages from the region of the Clarion-Clipperton Fracture Zone, an area of commercial mining interest.

PubMed

Lambshead, P John D; Brown, Caroline J; Ferrero, Timothy J; Hawkins, Lawrence E; Smith, Craig R; Mitchell, Nicola J

2003-01-09

The possibility for commercial mining of deep-sea manganese nodules is currently under exploration in the abyssal Clarion-Clipperton Fracture Zone. Nematodes have potential for biomonitoring of the impact of commercial activity but the natural biodiversity is unknown. We investigate the feasibility of nematodes as biomonitoring organisms and give information about their natural biodiversity. The taxonomic composition (at family to genus level) of the nematode fauna in the abyssal Pacific is similar, but not identical to, the North Atlantic. Given the immature state of marine nematode taxonomy, it is not possible to comment on the commonality or otherwise of species between oceans. The between basin differences do not appear to be directly linked to current ecological factors. The abyssal Pacific region (including the Fracture Zone) could be divided into two biodiversity subregions that conform to variations in the linked factors of flux to the benthos and of sedimentary characteristics. Richer biodiversity is associated with areas of known phytodetritus input and higher organic-carbon flux. Despite high reported sample diversity, estimated regional diversity is less than 400 species. The estimated regional diversity of the CCFZ is a tractable figure for biomonitoring of commercial activities in this region using marine nematodes, despite the immature taxonomy (i.e. most marine species have not been described) of the group. However, nematode ecology is in dire need of further study.

Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring.

PubMed

Woutersen, Marjolijn; van der Gaag, Bram; Abrafi Boakye, Afua; Mink, Jan; Marks, Robert S; Wagenvoort, Arco J; Ketelaars, Henk A M; Brouwer, Bram; Heringa, Minne B

2017-11-22

Surface water used for drinking water production is frequently monitored in The Netherlands using whole organism biomonitors, with for example Daphnia magna or Dreissena mussels, which respond to changes in the water quality. However, not all human-relevant toxic compounds can be detected by these biomonitors. Therefore, a new on-line biosensor has been developed, containing immobilized genetically modified bacteria, which respond to genotoxicity in the water by emitting luminescence. The performance of this sensor was tested under laboratory conditions, as well as under field conditions at a monitoring station along the river Meuse in The Netherlands. The sensor was robust and easy to clean, with inert materials, temperature control and nutrient feed for the reporter organisms. The bacteria were immobilized in sol-gel on either an optical fiber or a glass slide and then continuously exposed to water. Since the glass slide was more sensitive and robust, only this setup was used in the field. The sensor responded to spikes of genotoxic compounds in the water with a minimal detectable concentration of 0.01 mg/L mitomycin C in the laboratory and 0.1 mg/L mitomycin C in the field. With further optimization, which should include a reduction in daily maintenance, the sensor has the potential to become a useful addition to the currently available biomonitors.

Autopsy tissues as biological monitors of human exposure to environmental pollutants. A case study: Concentrations of metals and PCDD/Fs in subjects living near a hazardous waste incinerator.

PubMed

Domingo, José L; García, Francisco; Nadal, Martí; Schuhmacher, Marta

2017-04-01

Human biomonitoring is of tremendous importance to prevent potential adverse effects derived from human exposure to chemicals. Blood and urine are among the biological monitors more frequently used. However, biological matrices such as breast milk, hair, nails, saliva, feces, teeth, and expired air are also often used. In addition, and focused mainly on long-term exposure, adipose tissue and other human tissues like bone, liver, brain or kidney, are also used as biological monitors of certain substances, especially for long-term biomonitoring. However, for this kind of tissues sampling is always a limiting factor. In this paper, we have examined the role of autopsy tissues as biological monitors of human exposure to environmental pollutants. For it, we have used a case study conducted near a hazardous waste incinerator (HWI) in Catalonia (Spain), in which the concentrations of metals and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), have been periodically determined in autopsy tissues of subjects living in the area under potential influence of the facility. This case study does not show advantages -in comparison to other appropriate biomonitors such as blood- in using autopsy tissues in the monitoring of long-term exposure to metals and PCDD/Fs. Copyright © 2017 Elsevier Inc. All rights reserved.

Biomonitoring of styrene occupational exposures: Biomarkers and determinants.

PubMed

Persoons, Renaud; Richard, Justine; Herve, Claire; Montlevier, Sarah; Marques, Marie; Maitre, Anne

2018-06-22

High styrene exposures are still experienced in various occupational settings, requesting regular exposure assessments. The aims of this study were to study occupational exposures in various industrial sectors and to determine factors influencing styrene urinary metabolites levels. Biomonitoring was conducted in 141 workers from fiberglass-reinforced plastic (FRP) manufacture, thermoplastic polymers production, vehicle repair shops and cured-in-place pipe lining (CIPP). Urinary styrene (StyU) as well as Mandelic (MA) / Phenyglyoxylic Acids (PGA) were quantified at the beginning and at the end of week, and multivariate linear regression models were used. StyU levels revealed very low, rarely exceeding 3 µg.L -1 . Highest concentrations of MA + PGA were observed in FRP sector, with levels reaching up to 1100 mg.g -1 of creatinine. Factors influencing end-of-week MA + PGA concentrations were levels at the beginning of week, open molding processes, proximity to the emission source, respiratory protection, styrene content in raw materials. Elevated levels were also observed during CIPP process, whereas thermoplastic injection and vehicle repair shop workers exhibited much lower exposures. Intervention on process (decreasing styrene proportion, using closed molding), protective equipment (local exhaust ventilation, respiratory protection) and individual practices (stringent safety rules) are expected to decrease occupational exposures. Urinary MA + PGA remain the most appropriate biomarkers for occupational biomonitoring. Copyright © 2018. Published by Elsevier B.V.

Assessment of heavy metals in the particulate matter of two Brazilian metropolitan areas by using Tillandsia usneoides as atmospheric biomonitor.

PubMed

Vianna, Nelzair A; Gonçalves, Daniel; Brandão, Flavia; de Barros, Roberta P; Amado Filho, Gilberto M; Meire, Rodrigo O; Torres, João Paulo M; Malm, Olaf; D'Oliveira Júnior, Argemiro; Andrade, Leonardo R

2011-03-01

The aims of this paper were to quantify the heavy metals (HM) in the air of different sites in Rio de Janeiro (RJ) and Salvador (SA) using Tillandsia usneoides (Bromeliaceae) as a biomonitor, and to study the morphology and elemental composition of the air particulate matter (PM) retained on the Tillandsia surface. Tillandsia samples were collected in a noncontaminated area and exposed to the air of five sites in RJ State and seven in SA for 45 days, in two seasons. Samples were prepared to HM quantification by flame atomic absorption spectrophotometry, while morphological and elemental characterizations were studied by using scanning electron microscopy. HM concentrations were significantly higher when compared to control sites. We found an increasing metal concentration as follows: Cd < Cr < Pb < Cu < Zn. PM exhibited a morphology varying from amorphous- to polygonal-shaped particles. Size measurements indicated that more than 80% of particles were less than 10 μm. PM contained aluminosilicates iron-rich particles, but Zn, Cu, Cr, and Ba were also detected. HM input in the atmosphere was mainly associated with anthropogenic sources such as vehicle exhaust. Elemental analysis detected HM in the inhalable particles, indicating that those HMs may intensify the toxic effects of PM on human health. Our results indicated T. usneoides as an adequate biomonitor of HM in the PM belonging to the inhalable fraction.

Active biomonitoring with the moss Pseudoscleropodium purum: Comparison between different types of transplants and bulk deposition.

PubMed

Ares, A; Varela, Z; Aboal, J R; Carballeira, A; Fernández, J A

2015-10-01

Active biomonitoring with terrestrial mosses can be used to complement traditional air pollution monitoring techniques. Several studies have been carried out to compare the uptake capacity of different types of moss transplants. However, until now the relationship between the uptake of elements in devitalized moss bags and in irrigated transplants has not been explored. In this study, the final concentrations of Cd, Cu, Hg, Pb and Zn were determined in irrigated and devitalized moss transplants in the surroundings of a steelworks. The concentrations were also compared with those of the same elements in the bulk deposition to determine which type of moss transplant yields the closest correlations. Devitalized moss retained higher concentrations of all of the elements (except Hg) than the irrigated moss. Both irrigated and devitalized moss transplants appear to detect the same type of contamination (i.e. particulate matter and dissolved metals rather than gaseous forms) as significant correlations were found for Cu, Hg, Pb and Zn, whereas, neither type of the moss transplant was sensitive enough to detect changes in the soluble fraction load of bulk deposition. Further studies will be needed to a better understanding of the correlation between the concentrations of elements in moss transplants with the particulate fraction of the bulk deposition. This will enable the establishment of a more robust and accurate biomonitoring tool. Copyright © 2015 Elsevier Inc. All rights reserved.

A new way to contemplate Darwin's tangled bank: how DNA barcodes are reconnecting biodiversity science and biomonitoring.

PubMed

Hajibabaei, Mehrdad; Baird, Donald J; Fahner, Nicole A; Beiko, Robert; Golding, G Brian

2016-09-05

Encompassing the breadth of biodiversity in biomonitoring programmes has been frustrated by an inability to simultaneously identify large numbers of species accurately and in a timely fashion. Biomonitoring infers the state of an ecosystem from samples collected and identified using the best available taxonomic knowledge. The advent of DNA barcoding has now given way to the extraction of bulk DNA from mixed samples of organisms in environmental samples through the development of high-throughput sequencing (HTS). This DNA metabarcoding approach allows an unprecedented view of the true breadth and depth of biodiversity, but its adoption poses two important challenges. First, bioinformatics techniques must simultaneously perform complex analyses of large datasets and translate the results of these analyses to a range of users. Second, the insights gained from HTS need to be amalgamated with concepts such as Linnaean taxonomy and indicator species, which are less comprehensive but more intuitive. It is clear that we are moving beyond proof-of-concept studies to address the challenge of implementation of this new approach for environmental monitoring and regulation. Interpreting Darwin's 'tangled bank' through a DNA lens is now a reality, but the question remains: how can this information be generated and used reliably, and how does it relate to accepted norms in ecosystem study?This article is part of the themed issue 'From DNA barcodes to biomes'. © 2016 The Authors.

Assessing Diet as a Modifiable Risk Factor for Pesticide Exposure

PubMed Central

Oates, Liza; Cohen, Marc

2011-01-01

The effects of pesticides on the general population, largely as a result of dietary exposure, are unclear. Adopting an organic diet appears to be an obvious solution for reducing dietary pesticide exposure and this is supported by biomonitoring studies in children. However, results of research into the effects of organic diets on pesticide exposure are difficult to interpret in light of the many complexities. Therefore future studies must be carefully designed. While biomonitoring can account for differences in overall exposure it cannot necessarily attribute the source. Due diligence must be given to appropriate selection of participants, target pesticides and analytical methods to ensure that the data generated will be both scientifically rigorous and clinically useful, while minimising the costs and difficulties associated with biomonitoring studies. Study design must also consider confounders such as the unpredictable nature of chemicals and inter- and intra-individual differences in exposure and other factors that might influence susceptibility to disease. Currently the most useful measures are non-specific urinary metabolites that measure a range of organophosphate and synthetic pyrethroid insecticides. These pesticides are in common use, frequently detected in population studies and may provide a broader overview of the impact of an organic diet on pesticide exposure than pesticide-specific metabolites. More population based studies are needed for comparative purposes and improvements in analytical methods are required before many other compounds can be considered for assessment. PMID:21776202

Biomonitoring of the environmental genotoxic potential of emissions from a complex of ceramic industries in Monte Carmelo, Minas Gerais, Brazil, using Tradescantia pallida.

PubMed

Campos, Carlos Fernando; Júnior, Edimar Olegário de Campos; Souto, Henrique Nazareth; Sousa, Eduardo de Freitas; Pereira, Boscolli Barbosa

2016-01-01

The micronucleus (MN) test and analysis of heavy metal biological accumulation in Tradescantia pallida (T. pallida) were bioassays used to assess the genotoxic potential of emissions from a complex of ceramic industries into the atmosphere in a city in Brazil that is considered a national reference source for roof tile production. The ceramic industry emission-exposed T. pallida plants were biomonitored during the dry season, in June, July, and August 2013. In addition to the contaminated monitoring site, a reference site in a peri-urban area was utilized, for comparative purposes. Genotoxicity assessments were determined monthly, while heavy metal bioaccumulation was measured at the end of the total exposure period. The MN frequency was significantly greater in T. pallida plants exposed in the ceramic industry emission monitored area compared to the reference site, and highest MN rates were observed in July and August. With respect to heavy metal bioaccumulation in T. pallida leaves, cadmium (Cd) and chromium (Cr) concentrations were significantly higher in plants at the ceramic industry emission monitoring site. Thus, in relation to the parameters assessed, T. pallida was found to be sensitive to atmospheric contamination by heavy metals attributed to ceramic products emissions generated by the ceramic industry, confirming that this plant species may be employed as a reference organism in biomonitoring studies.

Biomonitoring of Trace Metals in the Keban Dam Reservoir (Turkey) Using Mussels (Unio elongatulus eucirrus) and Crayfish (Astacus leptodactylus).

PubMed

Varol, Memet; Sünbül, Muhammet Raşit

2018-01-03

Freshwater mussels and crayfish are commonly used as biomonitors of trace metals. In the present study, the concentrations of ten metals were determined in mussels (Unio elongatulus eucirrus) and crayfish (Astacus leptodactylus) collected from the Keban Dam Reservoir in Turkey. The significant spatial differences in concentrations of studied metals except As in mussels were not found. However, Co, Cr, Cu, and Zn concentrations in mussels and As, Co, Cu, Fe, Pb, and Zn concentrations in crayfish showed significant seasonal differences. As, Cd, and Mn levels in mussels were about nine times higher than those in crayfish. The concentrations of Cd, Cr, Cu, Pb, Zn, and inorganic As in crayfish and mussels were lower than maximum permissible levels. When compared with other biomonitoring studies using mussels and crayfish, high concentrations of As, Cd, Co, Cr, and Ni in mussels and Cr and Ni in crayfish were observed due to lithogenic sources and anthropogenic activities in the basin. Bioconcentration factor values of Fe, Mn, Cd, and Zn in mussels and Zn, Cu, Fe, and Co in crayfish were > 1000, which indicates that both U. e. eucirrus and A. leptodactylus have potential to bioaccumulate these metals. Therefore, attention should be paid to mussels and crayfish from ecological and human health perspective, because they are potential vectors of metals to higher trophic levels.

Assessing diet as a modifiable risk factor for pesticide exposure.

PubMed

Oates, Liza; Cohen, Marc

2011-06-01

The effects of pesticides on the general population, largely as a result of dietary exposure, are unclear. Adopting an organic diet appears to be an obvious solution for reducing dietary pesticide exposure and this is supported by biomonitoring studies in children. However, results of research into the effects of organic diets on pesticide exposure are difficult to interpret in light of the many complexities. Therefore future studies must be carefully designed. While biomonitoring can account for differences in overall exposure it cannot necessarily attribute the source. Due diligence must be given to appropriate selection of participants, target pesticides and analytical methods to ensure that the data generated will be both scientifically rigorous and clinically useful, while minimising the costs and difficulties associated with biomonitoring studies. Study design must also consider confounders such as the unpredictable nature of chemicals and inter- and intra-individual differences in exposure and other factors that might influence susceptibility to disease. Currently the most useful measures are non-specific urinary metabolites that measure a range of organophosphate and synthetic pyrethroid insecticides. These pesticides are in common use, frequently detected in population studies and may provide a broader overview of the impact of an organic diet on pesticide exposure than pesticide-specific metabolites. More population based studies are needed for comparative purposes and improvements in analytical methods are required before many other compounds can be considered for assessment.

A new way to contemplate Darwin's tangled bank: how DNA barcodes are reconnecting biodiversity science and biomonitoring

PubMed Central

Baird, Donald J.; Fahner, Nicole A.; Beiko, Robert; Golding, G. Brian

2016-01-01

Encompassing the breadth of biodiversity in biomonitoring programmes has been frustrated by an inability to simultaneously identify large numbers of species accurately and in a timely fashion. Biomonitoring infers the state of an ecosystem from samples collected and identified using the best available taxonomic knowledge. The advent of DNA barcoding has now given way to the extraction of bulk DNA from mixed samples of organisms in environmental samples through the development of high-throughput sequencing (HTS). This DNA metabarcoding approach allows an unprecedented view of the true breadth and depth of biodiversity, but its adoption poses two important challenges. First, bioinformatics techniques must simultaneously perform complex analyses of large datasets and translate the results of these analyses to a range of users. Second, the insights gained from HTS need to be amalgamated with concepts such as Linnaean taxonomy and indicator species, which are less comprehensive but more intuitive. It is clear that we are moving beyond proof-of-concept studies to address the challenge of implementation of this new approach for environmental monitoring and regulation. Interpreting Darwin's ‘tangled bank’ through a DNA lens is now a reality, but the question remains: how can this information be generated and used reliably, and how does it relate to accepted norms in ecosystem study? This article is part of the themed issue ‘From DNA barcodes to biomes’. PMID:27481782

Mount control system of the ASTRI SST-2M prototype for the Cherenkov Telescope Array

NASA Astrophysics Data System (ADS)

Antolini, Elisa; Tosti, Gino; Tanci, Claudio; Bagaglia, Marco; Canestrari, Rodolfo; Cascone, Enrico; Gambini, Giorgio; Nucciarelli, Giuliano; Pareschi, Giovanni; Scuderi, Salvo; Stringhetti, Luca; Busatta, Andrea; Giacomel, Stefano; Marchiori, Gianpietro; Manfrin, Cristiana; Marcuzzi, Enrico; Di Michele, Daniele; Grigolon, Carlo; Guarise, Paolo

2016-08-01

The ASTRI SST-2M telescope is an end-to-end prototype proposed for the Small Size class of Telescopes (SST) of the future Cherenkov Telescope Array (CTA). The prototype is installed in Italy at the INAF observing station located at Serra La Nave on Mount Etna (Sicily) and it was inaugurated in September 2014. This paper presents the software and hardware architecture and development of the system dedicated to the control of the mount, health, safety and monitoring systems of the ASTRI SST-2M telescope prototype. The mount control system installed on the ASTRI SST-2M telescope prototype makes use of standard and widely deployed industrial hardware and software. State of the art of the control and automation industries was selected in order to fulfill the mount related functional and safety requirements with assembly compactness, high reliability, and reduced maintenance. The software package was implemented with the Beckhoff TwinCAT version 3 environment for the software Programmable Logical Controller (PLC), while the control electronics have been chosen in order to maximize the homogeneity and the real time performance of the system. The integration with the high level controller (Telescope Control System) has been carried out by choosing the open platform communications Unified Architecture (UA) protocol, supporting rich data model while offering compatibility with the PLC platform. In this contribution we show how the ASTRI approach for the design and implementation of the mount control system has made the ASTRI SST-2M prototype a standalone intelligent machine, able to fulfill requirements and easy to be integrated in an array configuration such as the future ASTRI mini-array proposed to be installed at the southern site of the Cherenkov Telescope Array (CTA).

Technology for home dementia care: A prototype locating system put to the test.

PubMed

Megges, Herlind; Freiesleben, Silka Dawn; Jankowski, Natalie; Haas, Brigitte; Peters, Oliver

2017-09-01

The user experience of persons with dementia and their primary caregivers with locating systems is not firmly established. Eighteen dyads used a prototype locating system during 4 weeks. Primary outcome measures were ratings of usability, and product functions and features. Secondary outcome measures were caregiver burden, perceived self-efficacy, frequency of use, and willingness to purchase the prototype. Changes in scores between baseline (T 1 ) and end of testing period (T 2 ) were compared by performing independent and dependent samples correlations and descriptive statistics. Seventeen dyads made up the final sample. Ratings of usability and product functions and features were fair, but usability ratings were significantly reduced after 4 weeks. Although the prototype was used infrequently by majority of the participants, most caregivers would be willing to purchase the prototype, with men more willing than women. No significant change in technological willingness, caregiver burden, or perceived self-efficacy was found between T 1 and T 2 . Perceived self-efficacy significantly negatively correlated with willingness to purchase the prototype after 4 weeks. Results highlight the importance of including end users in the research and development phase of locating systems to improve the user experience in home dementia care. Necessary indications for further research are carrying out randomized controlled trials with larger, more representative samples and developing innovative software and hardware solutions.

Bio-monitoring of mycotoxin exposure in Cameroon using a urinary multi-biomarker approach.

PubMed

Abia, Wilfred A; Warth, Benedikt; Sulyok, Michael; Krska, Rudolf; Tchana, Angele; Njobeh, Patrick B; Turner, Paul C; Kouanfack, Charles; Eyongetah, Mbu; Dutton, Mike; Moundipa, Paul F

2013-12-01

Bio-monitoring of human exposure to mycotoxin has mostly been limited to a few individually measured mycotoxin biomarkers. This study aimed to determine the frequency and level of exposure to multiple mycotoxins in human urine from Cameroonian adults. 175 Urine samples (83% from HIV-positive individuals) and food frequency questionnaire responses were collected from consenting Cameroonians, and analyzed for 15 mycotoxins and relevant metabolites using LC-ESI-MS/MS. In total, eleven analytes were detected individually or in combinations in 110/175 (63%) samples including the biomarkers aflatoxin M1, fumonisin B1, ochratoxin A and total deoxynivalenol. Additionally, important mycotoxins and metabolites thereof, such as fumonisin B2, nivalenol and zearalenone, were determined, some for the first time in urine following dietary exposures. Multi-mycotoxin contamination was common with one HIV-positive individual exposed to five mycotoxins, a severe case of co-exposure that has never been reported in adults before. For the first time in Africa or elsewhere, this study quantified eleven mycotoxin biomarkers and bio-measures in urine from adults. For several mycotoxins estimates indicate that the tolerable daily intake is being exceeded in this study population. Given that many mycotoxins adversely affect the immune system, future studies will examine whether combinations of mycotoxins negatively impact Cameroonian population particularly immune-suppressed individuals. Copyright © 2013 Elsevier Ltd. All rights reserved.

Oxygen flux as an indicator of physiological stress in fathead minnow (Pimephales promelas) embryos: a real-time biomonitoring system of water quality.

PubMed

Sanchez, Brian C; Ochoa-Acuña, Hugo; Porterfield, D Marshall; Sepúlveda, María S

2008-09-15

The detection of harmful chemicals and biological agents in real time is a critical need for protecting freshwater ecosystems. We studied the real-time effects of five environmental contaminants with differing modes of action (atrazine, cadmium chloride, pentachlorophenol, malathion, and potassium cyanide) on respiratory oxygen consumption in 2-day postfertilization fathead minnow (Pimephales promelas) eggs. Our objective was to assess the sensitivity of fathead minnow eggs using the self-referencing micro-optrode technique to detect instantaneous changes in oxygen consumption after brief exposures to low concentrations of contaminants. Oxygen consumption data indicated that the technique is indeed sensitive enough to reliably detect physiological alterations induced by four of the five contaminants. After 2 h of exposure, we identified significant increases in oxygen consumption upon exposure to pentachlorophenol (100 and 1000 microg/L), cadmium chloride (0.0002 and 0.002 microg/L), and atrazine (150 microg/L). In contrast, we observed a significant decrease in oxygen flux after exposuresto potassium cyanide (44 and 66 microg/L) and atrazine (1500 microg/L). No effects were detected after exposures to malathion (200 and 340 microg/L). Our work is the first step in development of a new technique for physiologically coupled biomonitoring as a sensitive and reliable tool for the detection of environmental toxicants.

Biomonitoring of Organophosphorus Agent Exposure by Reactivation of Cholinesterase Enzyme Based on Carbon Nanotube-Enhanced Flow-Injection Amperometric Detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Du, Dan; Wang, Jun; Smith, Jordan N.

2009-11-15

A portable, rapid, and sensitive assessment of sub-clinical organophosphorus (OPs) agent exposure based on reactivation of cholinesterase (ChE) from OP-inhibited ChE using rat saliva (in vitro) was developed using an electrochemical sensor coupled with a microflow-injection system. The sensor was based on a carbon nanotube (CNT)-modified screen printed carbon electrode (SPE), which was integrated into a flow cell. Due to the extent of inter-individual ChE activity variability, ChE biomonitoring often requires an initial base-line determination (non-inhibited) of enzyme activity which is then directly compared with activity after OP exposure. This manuscript described an alternative strategy where reactivation of the phosphorylatedmore » enzyme was exploited to enable measurement of both inhibited and baseline ChE activity (i.e. after reactivation) in the same sample. The use of CNT makes the electrochemical detection of the products from enzymatic reactions more feasible with extremely high sensitivity and at low potentials. Paraoxon was selected as a model OP compound for in vitro inhibition studies. Some experiment parameters, (e.g. inhibition and reactivation times), have been optimized such that, 92 - 95% ChE reactivation can be achieved over a broad range of ChE inhibition (5 - 94 %) with paraoxon. The extent of enzyme inhibition using this electrochemical sensor correlates well with conventional enzyme activity measurements.« less

Online information system for data collection of cattle quality

NASA Astrophysics Data System (ADS)

Sugiharti, E.; Arifudin, R.; Putra, A. T.

2018-03-01

Innovation and development of the science and technology which proclaimed by the government through Ristekdikti need to be supported. On the other hand, the Department of Animal Husbandry and Fisheries began introducing the Cattle Card system that contains the identity of each farm animal. Therefore, UNNES especially the Department of Computer Science of FMIPA UNNES, need to give positive contribution in the field of Science and Technology to support the manual system of Cattle Card, through the preparation of prototype of the online information system of data collection of cattle in Semarang regency. The main problem is how to monitor the data of cattle quality through online information system in Semarang regency? The purpose of this research is to produce the prototype of an online information system for data collection of cattle quality in Semarang regency. Main activities: (1) Prepare the flowchart of an online system for data collection of cattle quality. (2) Collecting data to obtain data on identity descriptions of each cattle, owners, mutation records, and health records of livestock cattle. (3) Creation of the prototype of an online information system for data collection of cattle quality in Semarang Regency. The results, (1) had been produced the prototype of an online information system for data collection of cattle in the region of Semarang regency. (2) Socialization of the online information system for cattle quality data collection and exploring input from various related stakeholders. (3) There had been a limited trial of prototypes of the system in Pabelan district in the working area of the Department of Animal Husbandry and Fisheries of Semarang regency and succeeded well.

75 FR 71562 - Defense Federal Acquisition Regulation Supplement; Contract Authority for Advanced Component...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-24

... Development or Prototype Units (DFARS Case 2009-D034) AGENCY: Defense Acquisition Regulations System... the procurement of prototype units. The DFARS implementation places specific limits, in accordance... components or the procurement of prototype units. IV. Paperwork Reduction Act The Paperwork Reduction Act...

Expert operator's associate: A knowledge based system for spacecraft control

NASA Technical Reports Server (NTRS)

Nielsen, Mogens; Grue, Klaus; Lecouat, Francois

1991-01-01

The Expert Operator's Associate (EOA) project is presented which studies the applicability of expert systems for day-to-day space operations. A prototype expert system is developed, which operates on-line with an existing spacecraft control system at the European Space Operations Centre, and functions as an 'operator's assistant' in controlling satellites. The prototype is demonstrated using an existing real-time simulation model of the MARECS-B2 telecommunication satellite. By developing a prototype system, the extent to which reliability and effectivens of operations can be enhanced by AI based support is examined. In addition the study examines the questions of acquisition and representation of the 'knowledge' for such systems, and the feasibility of 'migration' of some (currently) ground-based functions into future spaceborne autonomous systems.

Office worker response to an automated venetian blind and electric lighting system: A pilot study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vine, E.; Lee, E.; Clear, R.

1998-03-01

A prototype integrated, dynamic building envelope and lighting system designed to optimize daylight admission and solar heat gain rejection on a real-time basis in a commercial office building is evaluated. Office worker response to the system and occupant-based modifications to the control system are investigated to determine if the design and operation of the prototype system can be improved. Key findings from the study are: (1) the prototype integrated envelope and lighting system is ready for field testing, (2) most office workers (N=14) were satisfied with the system, and (3) there were few complaints. Additional studies are needed to explainmore » how illuminance distribution, lighting quality, and room design can affect workplans illuminance preferences.« less

System design and architecture for the IDTO prototype – phase I demonstration site (Columbus).

DOT National Transportation Integrated Search

2013-11-01

This report documents the System Design and Architecture for the Phase I implementation of the Integrated Dynamic Transit Operations (IDTO) Prototype bundle within the Dynamic Mobility Applications (DMA) portion of the Connected Vehicle Program.

Missing: Prototype Vocational Guidance System.

ERIC Educational Resources Information Center

Weltin, Mary M.; Johnson, Richard M.

1985-01-01

Describes Army research in recruitment and vocational counseling. Discusses the Joint Optical Information Network, which offers career opportunity information, and the Army Research Institute's investigations of vocational counseling for high school and community college students and its prototype vocational guidance system. Encourages community…

System design and architecture for the IDTO prototype : phase II demonstration site (central Florida).

DOT National Transportation Integrated Search

2015-05-01

This report documents the System Design and Architecture for the Phase II implementation of the Integrated Dynamic Transit Operations (IDTO) Prototype bundle within the Dynamic Mobility Applications (DMA) portion of the Connected Vehicle Program. Thi...

A Prototype Expert System for Fishway Design.

ERIC Educational Resources Information Center

Bender, Michael J.; And Others

1992-01-01

Describes the development of a prototype expert system to recommend the most suitable type of fishway for given design conditions. Recommendations are provided on the basis of fishway hydraulics, fish passage performance, and cost requirements. An appendix provides an example consultation. (MDH)

Vehicle proximity alert system for highway-railroad grade crossings-prototype research

DOT National Transportation Integrated Search

2001-04-01

This report describes testing of prototype vehicle proximity alert system (VPAS) technologies, and presents and evaluates the results. The object was to determine the feasibility of VPAS for possible use in priority vehicles (i.e., emergency vehicles...

RIM as the data base management system for a material properties data base

NASA Technical Reports Server (NTRS)

Karr, P. H.; Wilson, D. J.

1984-01-01

Relational Information Management (RIM) was selected as the data base management system for a prototype engineering materials data base. The data base provides a central repository for engineering material properties data, which facilitates their control. Numerous RIM capabilities are exploited to satisfy prototype data base requirements. Numerical, text, tabular, and graphical data and references are being stored for five material types. Data retrieval will be accomplished both interactively and through a FORTRAN interface. The experience gained in creating and exercising the prototype will be used in specifying requirements for a production system.

Spacelab Data Processing Facility (SLDPF) quality assurance expert systems development

NASA Technical Reports Server (NTRS)

Kelly, Angelita C.; Basile, Lisa; Ames, Troy; Watson, Janice; Dallam, William

1987-01-01

Spacelab Data Processing Facility (SLDPF) expert system prototypes were developed to assist in the quality assurance of Spacelab and/or Attached Shuttle Payload (ASP) processed telemetry data. The SLDPF functions include the capturing, quality monitoring, processing, accounting, and forwarding of mission data to various user facilities. Prototypes for the two SLDPF functional elements, the Spacelab Output Processing System and the Spacelab Input Processing Element, are described. The prototypes have produced beneficial results including an increase in analyst productivity, a decrease in the burden of tedious analyses, the consistent evaluation of data, and the providing of concise historical records.

Spacelab Data Processing Facility (SLDPF) quality assurance expert systems development

NASA Technical Reports Server (NTRS)

Kelly, Angelita C.; Basile, Lisa; Ames, Troy; Watson, Janice; Dallam, William

1987-01-01

Spacelab Data Processing Facility (SLDPF) expert system prototypes have been developed to assist in the quality assurance of Spacelab and/or Attached Shuttle Payload (ASP) processed telemetry data. SLDPF functions include the capturing, quality monitoring, processing, accounting, and forwarding of mission data to various user facilities. Prototypes for the two SLDPF functional elements, the Spacelab Output Processing System and the Spacelab Input Processing Element, are described. The prototypes have produced beneficial results including an increase in analyst productivity, a decrease in the burden of tedious analyses, the consistent evaluation of data, and the providing of concise historical records.

Model prototype utilization in the analysis of fault tolerant control and data processing systems

NASA Astrophysics Data System (ADS)

Kovalev, I. V.; Tsarev, R. Yu; Gruzenkin, D. V.; Prokopenko, A. V.; Knyazkov, A. N.; Laptenok, V. D.

2016-04-01

The procedure assessing the profit of control and data processing system implementation is presented in the paper. The reasonability of model prototype creation and analysis results from the implementing of the approach of fault tolerance provision through the inclusion of structural and software assessment redundancy. The developed procedure allows finding the best ratio between the development cost and the analysis of model prototype and earnings from the results of this utilization and information produced. The suggested approach has been illustrated by the model example of profit assessment and analysis of control and data processing system.

Trace elements biomonitoring in a historical mining district (les Malines, France).

PubMed

Saunier, Jean-Baptiste; Losfeld, Guillaume; Freydier, Rémi; Grison, Claude

2013-11-01

The aim of this study is to investigate the trace elements (TE) contents of potential biomonitors in a historical Zn-Pb mining district: apiary products (honey, royal-jelly and beeswax) lichen and moss were sampled and analysed. In spite of high TE concentrations in mining waste and soil, apiary products are free of TE contamination originating from historical mining. Lichen/moss show high TE levels, which suggest atmospheric input of local dust. Pb isotopes analysis proved the origin of TE found in lichen/moss to be mainly mining waste. These results help discuss the choice of relevant organisms for monitoring TE in the environment and bring additional data on the potential impacts of brownfields left after mining, especially on food products from apiaries. Copyright © 2013 Elsevier Ltd. All rights reserved.

Prototypal Course in Newspaper Mangement: A Systems Analysis Approach to Teaching the Economic Context of Press Freedom in Modern Society.

ERIC Educational Resources Information Center

DeMott, John

The prototypal course in newspaper management described in this paper is based on systems analysis and the systems flow approach. The introductory section of the paper discusses the need for instruction in newspaper management, the concepts of the systems approach and systems flow and the way they relate to enterprise management, and specific…

Digital Charge Coupled Device (CCD) Camera System Architecture

NASA Astrophysics Data System (ADS)

Babey, S. K.; Anger, C. D.; Green, B. D.

1987-03-01

We propose a modeling system for generic objects in order to recognize different objects from the same category with only one generic model. The representation consists of a prototype, represented by parts and their configuration. Parts are modeled by superquadric volumetric primitives which are combined via Boolean operations to form objects. Variations between objects within a category are described by allowable changes in structure and shape deformations of prototypical parts. Each prototypical part and relation has a set of associated features that can be recognized in the images. These features are used for selecting models from the model data base. The selected hypothetical models are then verified on the geometric level by deforming the prototype in allowable ways to match the data. We base our design of the modeling system upon the current psychological theories of categorization and of human visual perception.

Solar heating and cooling system design and development

NASA Technical Reports Server (NTRS)

1978-01-01

The development of eight prototype solar heating and combined heating and cooling systems is reported. Manufacture, test, installation, maintenance, problem resolution, and monitoring the operation of prototype systems is included. Heating and cooling equipment for single family residential and commercial applications and eight operational test sites (four heating and four heating and cooling) is described.

Implementation of an Expert System for Instructional Design: Phase 2. Design Document & Technical Report.

ERIC Educational Resources Information Center

Merrill, M. David; Li, Zhongmin

The purpose of this project was to develop a prototype expert instructional design system (ID Expert) which would demonstrate the feasibility of a consultation system for use by inexperienced instructional designers. The prototype gathers information from the designer and then makes recommendations for instructional design decisions. The output of…

Mechanical Design and Optimization of Swarm-Capable UAV Launch Systems

DTIC Science & Technology

2015-06-01

stakeholders. The end result was the successful development and demonstration of a launching system prototype specifically developed to rapidly launch a...requirements for the stakeholders. The end result was the successful development and demonstration of a launching system prototype specifically developed to... Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 7 Conclusion 125 7.1 Summary of Findings

Development of a prototype fluid volume measurement system. [for urine volume measurement on space missions

NASA Technical Reports Server (NTRS)

Poppendiek, H. F.; Sabin, C. M.; Meckel, P. T.

1974-01-01

The research is reported in applying the axial fluid temperature differential flowmeter to a urine volume measurement system for space missions. The fluid volume measurement system is described along with the prototype equipment package. Flowmeter calibration, electronic signal processing, and typical void volume measurements are also described.

Using Vector and Extended Boolean Matching in an Expert System for Selecting Foster Homes.

ERIC Educational Resources Information Center

Fox, Edward A.; Winett, Sheila G.

1990-01-01

Describes FOCES (Foster Care Expert System), a prototype expert system for choosing foster care placements for children which integrates information retrieval techniques with artificial intelligence. The use of prototypes and queries in Prolog routines, extended Boolean matching, and vector correlation are explained, as well as evaluation by…

Installation package for SIMS prototype system 2, solar hot water

NASA Technical Reports Server (NTRS)

1978-01-01

The prototype system 2 solar hot water was designed for use in a single family dwelling and consists of the following subsystems: collector, storage, energy transport, and control. Guidelines are presented for utilization in the development of detailed installation plans and specifications. Instruction on operation, maintenance, and repair of the system is discussed.

Prototype solar heated hot water systems and double-walled heat exchangers: A collection of quarterly reports

NASA Technical Reports Server (NTRS)

1978-01-01

The plan schedule and status of multiple objectives to be achieved in the development, manufacture, installation, and maintenance of two solar heated hot water prototype systems and two heat exchangers are reported. A computer program developed to resolve problems and evaluate system performance is described.

Solar heating and cooling systems design and development. [prototype development

NASA Technical Reports Server (NTRS)

1977-01-01

The development of twelve prototype solar heating/cooling systems, six heating and six heating and cooling systems, two each for single family, multi-family, and commercial applications, is reported. Schedules and technical discussions, along with illustrations on the progress made from April 1, 1977 through June 30, 1977 are detailed.

A Prototype Greek Text to Greek Sign Language Conversion System

ERIC Educational Resources Information Center

Kouremenos, Dimitris; Fotinea, Stavroula-Evita; Efthimiou, Eleni; Ntalianis, Klimis

2010-01-01

In this article, a prototype Greek text to Greek Sign Language (GSL) conversion system is presented. The system is integrated into an educational platform that addresses the needs of teaching GSL grammar and was developed within the SYNENNOESE project (Efthimiou "et al." 2004a. Developing an e-learning platform for the Greek sign…

Portable Analytical Systems for On-Site Diagnosis of Exposure to Pesticides and Nerve Agents

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lin, Yuehe; Wang, Jun; Liu, Guodong

In this chapter, we summarize recent work in our laboratory on the development of sensitive portable analytical systems for use in on-site detection of exposure to organophosphate (OP) pesticides and chemical nerve agents. These systems are based on various nanomaterials functioning as transducers; recognition agents or labels and various elelectrochemical/immunoassay techniques. The studied nanomaterials included functionalized carbon nanotubes (CNT), zirconia nanoparticles (NPs) and quantum dots (QDs). Three biomarkers e.g. the free OPs, metabolites of OPs and protein-OP adducts in biological matrices have been employed for biomonitoring of OP exposure with our developed system. It has been found that the nanomaterial-basedmore » portable analytical systems have high sensitivity for the detection of the biomarkers, which suggest that these technologies offer great promise for the rapid and on-site detection and evaluation of OP exposure.« less

Main Engine Prototype Development for 2nd Generation RLV RS-83

NASA Technical Reports Server (NTRS)

Vilja, John; Fisher, Mark; Lyles, Garry M. (Technical Monitor)

2002-01-01

This presentation reports on the NASA project to develop a prototype for RS-83 engine designed for use on reusable launch vehicles (RLV). Topics covered include: program objectives, overview schedule, organizational chart, integrated systems engineering processes, requirement analysis, catastrophic engine loss, maintainability analysis tools, and prototype design analysis.

Lessons Learned from a Collaborative Sensor Web Prototype

NASA Technical Reports Server (NTRS)

Ames, Troy; Case, Lynne; Krahe, Chris; Hess, Melissa; Hennessy, Joseph F. (Technical Monitor)

2002-01-01

This paper describes the Sensor Web Application Prototype (SWAP) system that was developed for the Earth Science Technology Office (ESTO). The SWAP is aimed at providing an initial engineering proof-of-concept prototype highlighting sensor collaboration, dynamic cause-effect relationship between sensors, dynamic reconfiguration, and remote monitoring of sensor webs.

Reconceiving ISD: Three Perspectives on Rapid Prototyping as a Paradigm Shift.

ERIC Educational Resources Information Center

Rathbun, Gail A.; And Others

Confronting recent design challenges, instructional designers have latched onto adaptive procedural techniques from outside the Instructional Systems Design (ISD) field. This discussion of rapid prototyping (RP) examines the perspectives of: (1) the prototype as the designer"s cognitive tool; (2) the designer as co-inquirer; and (3) the…

Quick Prototyping of Educational Software: An Object-Oriented Approach.

ERIC Educational Resources Information Center

Wong, Simon C-H

1994-01-01

Introduces and demonstrates a quick-prototyping model for educational software development that can be used by teachers developing their own courseware using an object-oriented programming system. Development of a courseware package called "The Match-Maker" is explained as an example that uses HyperCard for quick prototyping. (Contains…

Automated Title Page Cataloging: A Feasibility Study.

ERIC Educational Resources Information Center

Weibel, Stuart; And Others

1989-01-01

Describes the design of a prototype rule-based system for the automation of descriptive cataloging from title pages. The discussion covers the results of tests of the prototype, major impediments to automatic cataloging from title pages, and prospects for further progress. The rules implemented in the prototype are appended. (16 references)…

NALDA (Naval Aviation Logistics Data Analysis) CAI (computer aided instruction)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Handler, B.H.; France, P.A.; Frey, S.C.

Data Systems Engineering Organization (DSEO) personnel developed a prototype computer aided instruction CAI system for the Naval Aviation Logistics Data Analysis (NALDA) system. The objective of this project was to provide a CAI prototype that could be used as an enhancement to existing NALDA training. The CAI prototype project was performed in phases. The task undertaken in Phase I was to analyze the problem and the alternative solutions and to develop a set of recommendations on how best to proceed. The findings from Phase I are documented in Recommended CAI Approach for the NALDA System (Duncan et al., 1987). Inmore » Phase II, a structured design and specifications were developed, and a prototype CAI system was created. A report, NALDA CAI Prototype: Phase II Final Report, was written to record the findings and results of Phase II. NALDA CAI: Recommendations for an Advanced Instructional Model, is comprised of related papers encompassing research on computer aided instruction CAI, newly developing training technologies, instructional systems development, and an Advanced Instructional Model. These topics were selected because of their relevancy to the CAI needs of NALDA. These papers provide general background information on various aspects of CAI and give a broad overview of new technologies and their impact on the future design and development of training programs. The paper within have been index separately elsewhere.« less

Tactical Aviation Mission System Simulation Situational Awareness Project

DTIC Science & Technology

2004-04-01

prototyping and exercising human-machine systems and for measuring the impact of new technologies in a dynamic simulation environment. Theoretical...31 2.4.1 The Impact of an ERSTA-Like System on the CH-146 Mission Commander...was proven to be an effective platform for prototyping and exercising systems and for measuring the impact of new technologies in a dynamic simulation

Study and prototype of data system interactions for the Earth Observing System Data and Information System

NASA Technical Reports Server (NTRS)

Emmitt, G. D.; Wood, S. A.; Morris, M.

1990-01-01

A crucial part of the Earth Observing System (EOS) is its Data and Information System (EOSDIS). The success of EOS depends not only on its instruments and science studies, but also on its ability to help scientists integrate data sets of geophysical and biological measurements taken by various instruments and investigators. NASA contractors have completed Phase B studies of EOSDIS, in particular its architecture, functionality, and user interfacing. At this point in time, it may seem impossible to exercise the EOSDIS or any of its components since they do not exist; i.e., if the EOSDIS is accepted as a totally new system, distinct from any existing DIS. However, if EOSDIS is seen as evolving from existing data systems, then some limited prototyping studies can be conducted by using currently functioning systems. In support of both the EOSDIS Science Advisory Panel and the EOSDIS Project, a prototyping activity was carried out by a cross section of interdisciplinary scientists. That prototyping activity is summarized and some conclusions are drawn that can be used by NASA-Goddard to evaluate and modify the specifications soon to be released in an RFP to build EOSDIS.

The Development of a Programming Support System for Rapid Prototyping. Tasks 2 and 3.

DTIC Science & Technology

1985-01-01

of the problems that played a role in the larger system. The principal work in Task 2 was the design of a new method for code-generation...develop- Ing a prototype of the interpreter. Due to the limitation of funds, only a design , not a prototype of the bi-directional l 2 scanner was...Introduction LiTmitUn uupii The Problem of designing a code generator for an interpreter-based languae Jas provided a chance to reoexamine fth

Pilot study testing a European human biomonitoring framework for biomarkers of chemical exposure in children and their mothers: experiences in the UK.

PubMed

Exley, Karen; Aerts, Dominique; Biot, Pierre; Casteleyn, Ludwine; Kolossa-Gehring, Marike; Schwedler, Gerda; Castaño, Argelia; Angerer, Jürgen; Koch, Holger M; Esteban, Marta; Schindler, Birgit K; Schoeters, Greet; Den Hond, Elly; Horvat, Milena; Bloemen, Louis; Knudsen, Lisbeth E; Joas, Reinhard; Joas, Anke; Sepai, Ovnair

2015-10-01

Exposure to a number of environmental chemicals in UK mothers and children has been assessed as part of the European biomonitoring pilot study, Demonstration of a Study to Coordinate and Perform Human Biomonitoring on a European Scale (DEMOCOPHES). For the European-funded project, 17 countries tested the biomonitoring guidelines and protocols developed by COPHES. The results from the pilot study in the UK are presented; 21 school children aged 6-11 years old and their mothers provided hair samples to measure mercury and urine samples, to measure cadmium, cotinine and several phthalate metabolites: mono(2-ethyl-5-hydroxyhexyl)phthalate (5OH-MEHP), mono(2-ethyl-5-oxo-hexyl)phthalate (5oxo-MEHP) and mono(2-ethylhexyl)phthalate (MEHP), mono-ethyl phthalate (MEP), mono-iso-butyl phthalate (MiBP), mono-benzyl phthalate (MBzP) and mono-n-butyl phthalate (MnBP). Questionnaire data was collected on environment, health and lifestyle. Mercury in hair was higher in children who reported frequent consumption of fish (geometric mean 0.35 μg/g) compared to those that ate fish less frequently (0.13 μg/g, p = 0.002). Cadmium accumulates with age as demonstrated by higher levels of urinary cadmium in the mothers (geometric mean 0.24 μg/L) than in the children(0.14 μg/L). None of the mothers reported being regular smokers, and this was evident with extremely low levels of cotinine measured (maximum value 3.6 μg/L in mothers, 2.4 μg/L in children). Very low levels of the phthalate metabolites were also measured in both mothers and children (geometric means in mothers: 5OH-MEHP 8.6 μg/L, 5oxo-MEHP 5.1 μg/L, MEHP 1.2 μg/L, MEP 26.8 μg/L, MiBP 17.0 μg/L, MBzP 1.6 μg/L and MnBP 13.5 μg/L; and in children: 5OH-MEHP 18.4 μg/L, 5oxo-MEHP 11.4 μg/L, MEHP 1.4 μg/L, MEP 14.3 μg/L, MiBP 25.8 μg/L, MBzP 3.5 μg/L and MnBP 22.6 μg/L). All measured biomarker levels were similar to or below population-based reference values published by the US National Health and Nutrition Examination Survey (NHANES) and Germany's GerES surveys. No results were above available health guidance values and were of no concern with regards to health. The framework and techniques learnt here will assist with future work on biomonitoring in the UK.

Biochemical Study of Mixed Culture Prototype in a Closed Ecological System

NASA Technical Reports Server (NTRS)

Tischer, R. G.

1960-01-01

Since June 1, 1960, the date of initiation of this research project, efforts have been directed toward studying cultural and fermentation patterns and the methodology of pure culture isolation of prototype microorganisms to be employed in closed ecological systems.

A clinical decision support system prototype for cardiovascular intensive care.

PubMed

Lau, F

1994-08-01

This paper describes the development and validation of a decision-support system prototype that can help manage hypovolemic hypotension in the Cardiovascular Intensive Care Unit (CVICU). The prototype uses physiologic pattern-matching, therapeutic protocols, computational drug-dosage response modeling and expert reasoning heuristics in its selection of intervention strategies and choices. As part of model testing, the prototype simulated real-time operation by processing historical physiologic and intervention data on a patient sequentially, generating alerts on questionable data, critiques of interventions instituted and recommendations on preferred interventions. Bench-testing with 399 interventions from 13 historical cases showed therapies for bleeding and fluid replacement proposed by the prototype were significantly more consistent (p < 0.0001) than those instituted by the staff when compared against expert critiques (80% versus 44%). This study has demonstrated the feasibility of formalizing hemodynamic management of CVICU patients in a manner that may be implemented and evaluated in a clinical setting.

PANDA Muon System Prototype

NASA Astrophysics Data System (ADS)

Abazov, Victor; Alexeev, Gennady; Alexeev, Maxim; Frolov, Vladimir; Golovanov, Georgy; Kutuzov, Sergey; Piskun, Alexei; Samartsev, Alexander; Tokmenin, Valeri; Verkheev, Alexander; Vertogradov, Leonid; Zhuravlev, Nikolai

2018-04-01

The PANDA Experiment will be one of the key experiments at the Facility for Antiproton and Ion Research (FAIR) which is under construction now in the territory of the GSI Helmholtz Centre for Heavy Ion Research in Darmstadt, Germany. PANDA is aimed to study hadron spectroscopy and various topics of the weak and strong forces. Muon System is chosen as the most suitable technology for detecting the muons. The Prototype of the PANDA Muon System is installed on the test beam line T9 at the Proton Synchrotron (PS) at CERN. Status of the PANDA Muon System prototype is presented with few preliminary results.

The Space Station Prototype Program - The development of a regenerative life support system for extended-duration missions.

NASA Technical Reports Server (NTRS)

Willis, N. C., Jr.; Neel, J. M.

1972-01-01

Design concepts and test philosophies which may contribute to the development of a low-cost maintainable environmental control/life support system are examined. It is shown that the concept of producing flight prototype equipment during a developmental program can reduce the eventual cost of a flight system by incorporating realistic flight-type design requirements without imposing exacting design features and stringent controls. A flight prototype design is one that can be converted readily into an actual flight design without any conceptual change. Modularity of subsystems provides the system and the program a degree of flexibility relative to the eventual vehicle configuration and technological improvements.

Development of a vibration isolation prototype system for microgravity space experiments

NASA Technical Reports Server (NTRS)

Logsdon, Kirk A.; Grodsinsky, Carlos M.; Brown, Gerald V.

1990-01-01

The presence of small levels of low-frequency accelerations on the space shuttle orbiters has degraded the microgravity environment for the science community. Growing concern about this microgravity environment has generated interest in systems that can isolate microgravity science experiments from vibrations. This interest has resulted primarily in studies of isolation systems with active methods of compensation. The development of a magnetically suspended, six-degree-of-freedom active vibration isolation prototype system capable of providing the needed compensation to the orbital environment is presented. A design for the magnetic actuators is described, and the control law for the prototype system that gives a nonintrusive inertial isolation response to the system is also described. Relative and inertial sensors are used to provide an inertial reference for isolating the payload.

System analysis of graphics processor architecture using virtual prototyping

NASA Astrophysics Data System (ADS)

Hancock, William R.; Groat, Jeff; Steeves, Todd; Spaanenburg, Henk; Shackleton, John

1995-06-01

Honeywell has been actively involved in the definition of the next generation display processors for military and commercial cockpits. A major concern is how to achieve super graphics workstation performance in avionics application. Most notable are requirements for low volume, low power, harsh environmental conditions, real-time performance and low cost. This paper describes the application of VHDL to the system analysis tasks associated with achieving these goals in a cost effective manner. The paper will describe the top level architecture identified to provide the graphical and video processing power needed to drive future high resolution display devices and to generate more natural panoramic 3D formats. The major discussion, however, will be on the use of VHDL to model the processing elements and customized pipelines needed to realize the architecture and for doing the complex system tradeoff studies necessary to achieve a cost effective implementation. New software tools have been developed to allow 'virtual' prototyping in the VHDL environment. This results in a hardware/software codesign using VHDL performance and functional models. This unique architectural tool allows simulation and tradeoffs within a standard and tightly integrated toolset, which eventually will be used to specify and design the entire system from the top level requirements and system performance to the lowest level individual ASICs. New processing elements, algorithms, and standard graphical inputs can be designed, tested and evaluated without the costly hardware prototyping using the innovative 'virtual' prototyping techniques which are evolving on this project. In addition, virtual prototyping of the display processor does not bind the preliminary design to point solutions as a physical prototype will. when the development schedule is known, one can extrapolate processing elements performance and design the system around the most current technology.

Chesapeake Inundation Prediction System (CIPS): A regional prototype for a national problem

USGS Publications Warehouse

Stamey, B.; Smith, W.; Carey, K.; Garbin, D.; Klein, F.; Wang, Hongfang; Shen, J.; Gong, W.; Cho, J.; Forrest, D.; Friedrichs, C.; Boicourt, W.; Li, M.; Koterba, M.; King, D.; Titlow, J.; Smith, E.; Siebers, A.; Billet, J.; Lee, J.; Manning, Douglas R.; Szatkowski, G.; Wilson, D.; Ahnert, P.; Ostrowski, J.

2007-01-01

Recent Hurricanes Katrina and Isabel, among others, not only demonstrated their immense destructive power, but also revealed the obvious, crucial need for improved storm surge forecasting and information delivery to save lives and property in future storms. Current operational methods and the storm surge and inundation products do not adequately meet requirements needed by Emergency Managers (EMs) at local, state, and federal levels to protect and inform our citizens. The Chesapeake Bay Inundation Prediction System (CIPS) is being developed to improve the accuracy, reliability, and capability of flooding forecasts for tropical cyclones and non-tropical wind systems such as nor'easters by modeling and visualizing expected on-land storm-surge inundation along the Chesapeake Bay and its tributaries. An initial prototype has been developed by a team of government, academic and industry partners through the Chesapeake Bay Observing System (CBOS) of the Mid-Atlantic Coastal Ocean Observing Regional Association (MACOORA) within the Integrated Ocean Observing System (IOOS). For demonstration purposes, this initial prototype was developed for the tidal Potomac River in the Washington, DC metropolitan area. The preliminary information from this prototype shows great potential as a mechanism by which NOAA National Weather Service (NWS) Forecast Offices (WFOs) can provide more specific and timely forecasts of likely inundation in individual localities from significant storm surge events. This prototype system has shown the potential to indicate flooding at the street level, at time intervals of an hour or less, and with vertical resolution of one foot or less. This information will significantly improve the ability of EMs and first responders to mitigate life and property loss and improve evacuation capabilities in individual communities. This paper provides an update and expansion of the initial prototype that was presented at the Oceans 2006 MTS/IEEE Conference in Boston, MA. ??2007 MTS.

Prototypes and system test stands for the Phase 1 upgrade of the CMS pixel detector

DOE PAGES

Hasegawa, S.

2016-04-23

The CMS pixel phase-1 upgrade project replaces the current pixel detector with an upgraded system with faster readout electronics during the extended year-end technical stop of 2016/2017. New electronics prototypes for the system have been developed, and tests in a realistic environment for a comprehensive evaluation are needed. A full readout test stand with either the same hardware as used in the current CMS pixel detector or the latest prototypes of upgrade electronics has been built. The setup enables the observation and investigation of a jitter increase in the data line associated with trigger rate increases. This effect is duemore » to the way in which the clock and trigger distribution is implemented in CMS. A new prototype of the electronics with a PLL based on a voltage controlled quartz crystal oscillator (QPLL), which works as jitter filter, in the clock distribution path was produced. With the test stand, it was confirmed that the jitter increase is not seen with the prototype, and also good performance was confirmed at the expected detector operation temperature ($-$20 °C).« less

Priming for performance: valence of emotional primes interact with dissociable prototype learning systems.

PubMed

Gorlick, Marissa A; Maddox, W Todd

2013-01-01

Arousal Biased Competition theory suggests that arousal enhances competitive attentional processes, but makes no strong claims about valence effects. Research suggests that the scope of enhanced attention depends on valence with negative arousal narrowing and positive arousal broadening attention. Attentional scope likely affects declarative-memory-mediated and perceptual-representation-mediated learning systems differently, with declarative-memory-mediated learning depending on narrow attention to develop targeted verbalizable rules, and perceptual-representation-mediated learning depending on broad attention to develop a perceptual representation. We hypothesize that negative arousal accentuates declarative-memory-mediated learning and attenuates perceptual-representation-mediated learning, while positive arousal reverses this pattern. Prototype learning provides an ideal test bed as dissociable declarative-memory and perceptual-representation systems mediate two-prototype (AB) and one-prototype (AN) prototype learning, respectively, and computational models are available that provide powerful insights on cognitive processing. As predicted, we found that negative arousal narrows attentional focus facilitating AB learning and impairing AN learning, while positive arousal broadens attentional focus facilitating AN learning and impairing AB learning.

Priming for Performance: Valence of Emotional Primes Interact with Dissociable Prototype Learning Systems

PubMed Central

Gorlick, Marissa A.; Maddox, W. Todd

2013-01-01

Arousal Biased Competition theory suggests that arousal enhances competitive attentional processes, but makes no strong claims about valence effects. Research suggests that the scope of enhanced attention depends on valence with negative arousal narrowing and positive arousal broadening attention. Attentional scope likely affects declarative-memory-mediated and perceptual-representation-mediated learning systems differently, with declarative-memory-mediated learning depending on narrow attention to develop targeted verbalizable rules, and perceptual-representation-mediated learning depending on broad attention to develop a perceptual representation. We hypothesize that negative arousal accentuates declarative-memory-mediated learning and attenuates perceptual-representation-mediated learning, while positive arousal reverses this pattern. Prototype learning provides an ideal test bed as dissociable declarative-memory and perceptual-representation systems mediate two-prototype (AB) and one-prototype (AN) prototype learning, respectively, and computational models are available that provide powerful insights on cognitive processing. As predicted, we found that negative arousal narrows attentional focus facilitating AB learning and impairing AN learning, while positive arousal broadens attentional focus facilitating AN learning and impairing AB learning. PMID:23646101

Robotic Transnasal Endoscopic Skull Base Surgery: Systematic Review of the Literature and Report of a Novel Prototype for a Hybrid System (Brescia Endoscope Assistant Robotic Holder).

PubMed

Bolzoni Villaret, Andrea; Doglietto, Francesco; Carobbio, Andrea; Schreiber, Alberto; Panni, Camilla; Piantoni, Enrico; Guida, Giovanni; Fontanella, Marco Maria; Nicolai, Piero; Cassinis, Riccardo

2017-09-01

Although robotics has already been applied to several surgical fields, available systems are not designed for endoscopic skull base surgery (ESBS). New conception prototypes have been recently described for ESBS. The aim of this study was to provide a systematic literature review of robotics for ESBS and describe a novel prototype developed at the University of Brescia. PubMed and Scopus databases were searched using a combination of terms, including Robotics OR Robot and Surgery OR Otolaryngology OR Skull Base OR Holder. The retrieved papers were analyzed, recording the following features: interface, tools under robotic control, force feedback, safety systems, setup time, and operative time. A novel hybrid robotic system has been developed and tested in a preclinical setting at the University of Brescia, using an industrial manipulator and readily available off-the-shelf components. A total of 11 robotic prototypes for ESBS were identified. Almost all prototypes present a difficult emergency management as one of the main limits. The Brescia Endoscope Assistant Robotic holder has proven the feasibility of an intuitive robotic movement, using the surgeon's head position: a 6 degree of freedom sensor was used and 2 light sources were added to glasses that were therefore recognized by a commercially available sensor. Robotic system prototypes designed for ESBS and reported in the literature still present significant technical limitations. Hybrid robot assistance has a huge potential and might soon be feasible in ESBS. Copyright © 2017 Elsevier Inc. All rights reserved.

The effects of sleep deprivation on dissociable prototype learning systems.

PubMed

Maddox, W Todd; Glass, Brian D; Zeithamova, Dagmar; Savarie, Zachary R; Bowen, Christopher; Matthews, Michael D; Schnyer, David M

2011-03-01

The cognitive neural underpinnings of prototype learning are becoming clear. Evidence points to 2 different neural systems, depending on the learning parameters. A/not-A (AN) prototype learning is mediated by posterior brain regions that are involved in early perceptual learning, whereas A/B (AB) is mediated by frontal and medial temporal lobe regions. To investigate the effects of sleep deprivation on AN and AB prototype learning and to use established prototype models to provide insights into the cognitive-processing locus of sleep-deprivation deficits. Participants performed an AN and an AB prototype learning task twice, separated by a 24-hour period, with or without sleep between testing sessions. Eighteen West Point cadets participated in the sleep-deprivation group, and 17 West Point cadets participated in a control group. Sleep deprivation led to an AN, but not an AB, performance deficit. Prototype model analyses indicated that the AN deficit was due to changes in attentional focus and a decrease in confidence that is reflected in an increased bias to respond non-A. The findings suggest that AN, but not AB, prototype learning is affected by sleep deprivation. Prototype model analyses support the notion that the effect of sleep deprivation on AN is consistent with lapses in attentional focus that are more detrimental to AN than to AB. This finding adds to a growing body of work that suggests that different performance changes associated with sleep deprivation can be attributed to a common mechanism of changes in simple attention and vigilance.

Review, Selection and Installation of a Rapid Prototype Machine

NASA Technical Reports Server (NTRS)

McEndree, Caryl

2008-01-01

The objective of this paper is to impress upon the reader the benefits and advantages of investing in rapid prototyping (additive manufacturing) technology thru the procurement of one or two new rapid prototyping machines and the creation of a new Prototype and Model Lab at the Kennedy Space Center (KSC). This new resource will be available to all of United Space Alliance, LLC (USA), enabling engineers from around the company to pursue a more effective means of communication and design with our co-workers, and our customer, the National Aeronautics and Space Administration (NASA). The Rapid Protoyping/3D printing industry mirrors the transition the CAD industry made several years ago, when companies were trying to justify the expenditure of converting to a 3D based system from a 2D based system. The advantages of using a 3D system seemed to be outweighed by the cost it would take to convert not only legacy 2D drawings into 3D models but the training of personnel to use the 3D CAD software. But the reality was that when a 3D CAD system is employed, it gives engineers a much greater ability to conceive new designs and the ability to engineer new tools and products much more effectively. Rapid Prototyping (RP) is the name given to a host of related technologies that are used to fabricate physical objects directly from Computer Aided Design (CAD) data sources. These methods are generally similar to each other in that they add and bond materials in a layer wise-fashion to form objects, instead of machining away material. The machines used in Rapid Prototyping are also sometimes referred to as Rapid Manufacturing machines due to the fact that some of the parts fabricated in a RP machine can be used as the finished product. The name "Rapid Prototyping" is really a misnomer. It is much more than prototypes and it is not always rapid.