{gamma}-RAY AND PARSEC-SCALE JET PROPERTIES OF A COMPLETE SAMPLE OF BLAZARS FROM THE MOJAVE PROGRAM

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lister, M. L.; Hovatta, T.; Aller, M.

We investigate the Fermi Large Area Telescope {gamma}-ray and 15 GHz Very Long Baseline Array radio properties of a joint {gamma}-ray and radio-selected sample of active galactic nuclei (AGNs) obtained during the first 11 months of the Fermi mission (2008 August 4-2009 July 5). Our sample contains the brightest 173 AGNs in these bands above declination -30 Degree-Sign during this period, and thus probes the full range of {gamma}-ray loudness ({gamma}-ray to radio band luminosity ratio) in the bright blazar population. The latter quantity spans at least 4 orders of magnitude, reflecting a wide range of spectral energy distribution (SED)more » parameters in the bright blazar population. The BL Lac objects, however, display a linear correlation of increasing {gamma}-ray loudness with synchrotron SED peak frequency, suggesting a universal SED shape for objects of this class. The synchrotron self-Compton model is favored for the {gamma}-ray emission in these BL Lac objects over external seed photon models, since the latter predict a dependence of Compton dominance on Doppler factor that would destroy any observed synchrotron SED-peak-{gamma}-ray-loudness correlation. The high-synchrotron peaked (HSP) BL Lac objects are distinguished by lower than average radio core brightness temperatures, and none display large radio modulation indices or high linear core polarization levels. No equivalent trends are seen for the flat-spectrum radio quasars (FSRQs) in our sample. Given the association of such properties with relativistic beaming, we suggest that the HSP BL Lac objects have generally lower Doppler factors than the lower-synchrotron peaked BL Lac objects or FSRQs in our sample.« less

Properties of optically selected BL Lacertae candidates from the SDSS

NASA Astrophysics Data System (ADS)

Kügler, S. D.; Nilsson, K.; Heidt, J.; Esser, J.; Schultz, T.

2014-09-01

Context. Deep optical surveys open the avenue for finding large numbers of BL Lac objects that are hard to identify because they lack the unique properties classifying them as such. While radio or X-ray surveys typically reveal dozens of sources, recent compilations based on optical criteria alone have increased the number of BL Lac candidates considerably. However, these compilations are subject to biases and may contain a substantial number of contaminating sources. Aims: In this paper we extend our analysis of 182 optically selected BL Lac object candidates from the SDSS with respect to an earlier study. The main goal is to determine the number of bona fide BL Lac objects in this sample. Methods: We examine their variability characteristics, determine their broad-band radio-UV spectral energy distributions (SEDs), and search for the presence of a host galaxy. In addition we present new optical spectra for 27 targets with improved signal-to-noise ratio with respect to the SDSS spectra. Results: At least 59% of our targets have shown variability between SDSS DR2 and our observations by more than 0.1-0.27 mag depending on the telescope used. A host galaxy was detected in 36% of our targets. The host galaxy type and luminosities are consistent with earlier studies of BL Lac host galaxies. Simple fits to broad-band SEDs for 104 targets of our sample derived synchrotron peak frequencies between 13.5 ≤ log 10(νpeak) ≤ 16 with a peak at log 10 ~ 14.5. Our new optical spectra do not reveal any new redshift for any of our objects. Thus the sample contains a large number of bona fide BL Lac objects and seems to contain a substantial fraction of intermediate-frequency peaked BL Lacs. Based on observations collected with the NTT on La Silla (Chile) operated by the European Southern Observatory under proposal 082.B-0133.Based on observations collected at the Centro Astronómico Hispano Alemán (CAHA), operated jointly by the Max-Planck-Institut für Astronomie and the Instituto de Astrofisica de Canarias.Based on observations made with the Nordic Optical Telescope, operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway, and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias.

Time-dependent inhomogeneous jet models for BL Lac objects

NASA Technical Reports Server (NTRS)

Marlowe, A. T.; Urry, C. M.; George, I. M.

1992-01-01

Relativistic beaming can explain many of the observed properties of BL Lac objects (e.g., rapid variability, high polarization, etc.). In particular, the broadband radio through X-ray spectra are well modeled by synchrotron-self Compton emission from an inhomogeneous relativistic jet. We have done a uniform analysis on several BL Lac objects using a simple but plausible inhomogeneous jet model. For all objects, we found that the assumed power-law distribution of the magnetic field and the electron density can be adjusted to match the observed BL Lac spectrum. While such models are typically unconstrained, consideration of spectral variability strongly restricts the allowed parameters, although to date the sampling has generally been too sparse to constrain the current models effectively. We investigate the time evolution of the inhomogeneous jet model for a simple perturbation propagating along the jet. The implications of this time evolution model and its relevance to observed data are discussed.

Time-dependent inhomogeneous jet models for BL Lac objects

NASA Astrophysics Data System (ADS)

Marlowe, A. T.; Urry, C. M.; George, I. M.

1992-05-01

Relativistic beaming can explain many of the observed properties of BL Lac objects (e.g., rapid variability, high polarization, etc.). In particular, the broadband radio through X-ray spectra are well modeled by synchrotron-self Compton emission from an inhomogeneous relativistic jet. We have done a uniform analysis on several BL Lac objects using a simple but plausible inhomogeneous jet model. For all objects, we found that the assumed power-law distribution of the magnetic field and the electron density can be adjusted to match the observed BL Lac spectrum. While such models are typically unconstrained, consideration of spectral variability strongly restricts the allowed parameters, although to date the sampling has generally been too sparse to constrain the current models effectively. We investigate the time evolution of the inhomogeneous jet model for a simple perturbation propagating along the jet. The implications of this time evolution model and its relevance to observed data are discussed.

Spectroscopic and polarimetric study of radio-quiet weak emission line quasars

NASA Astrophysics Data System (ADS)

Kumar, Parveen; Chand, Hum; Gopal-Krishna; Srianand, Raghunathan; Stalin, Chelliah Subramonian; Petitjean, Patrick

2018-04-01

A small subset of optically selected radio-quiet QSOs with weak or no emission lines may turn out to be the elusive radio-quiet BL Lac objects, or simply be radio-quiet QSOs with an infant/shielded broad line region (BLR). High polarisation (p > 3-4%), a hallmark of BL Lacs, can be used to test whether some optically selected ‘radio-quiet weak emission line QSOs’ (RQWLQs) show a fractional polarisation high enough to qualify as radio-quiet analogues of BL Lac objects. To check this possibility, we have made optical spectral and polarisation measurements of a sample of 19 RQWLQs. Out of these, only 9 sources show a non-significant proper motion (hence very likely extragalactic) and only two of them are found to have p > 1%. For these two RQWLQs, namely J142505.59+035336.2 and J154515.77+003235.2, we found the highest polarization to be 1.59±0.53%, which is again too low to classify them as (radio-quiet) BL Lacs, although one may recall that even genuine BL Lacs sometimes appear weakly polarised. We also present a statistical comparison of the optical spectral index, for a sample of 45 RQWLQs with redshift-luminosity matched control samples of 900 QSOs and an equivalent sample of 120 blazars, assembled from the literature. The spectral index distribution of RQWLQs is found to differ, at a high significance level, from that of blazars. This, too, is consistent with the common view that the mechanism of the central engine in RQWLQs, as a population, is close to that operating in normal QSOs and the primary difference between them is related to the BLR.

A KPC-scale X-ray jet in the BL LAC Source S5 2007+777

NASA Technical Reports Server (NTRS)

Sambruna, Rita; Maraschi, Laura; Tavecchio, Fabrizio

2008-01-01

The BL Lac S3 2007++777, a classical radio-selected BL Lac from the sample of Stirkel et al. exhibiting an extended (19") radio jet. was observed with Chandra revealing an X-ray jet with simi1ar morphology. The hard X-ray spectrum and broad band SED is consistent with an IC/CMB origin for the X-ray emission, implying a highly relativistic flow at small angle to the line of sight with an unusually large deprojected length, 300 kpc. A structured jet consisting of a fast spine and slow wall is consistent with the observations.

X-ray flux variability of active galactic nuclei observed using NuSTAR

NASA Astrophysics Data System (ADS)

Rani, Priyanka; Stalin, C. S.; Rakshit, Suvendu

2017-04-01

We present results of a systematic study of flux variability on hourly time-scales in a large sample of active galactic nuclei (AGN) in the 3-79 keV band using data from Nuclear Spectroscopic Telescope Array. Our sample consists of four BL Lac objects (BL Lacs), three flat spectrum radio quasars (FSRQs) 24 Seyfert 1, 42 Seyfert 2 and eight narrow line Seyfert 1 (NLSy1) galaxies. We find that in the 3-79 keV band, about 65 per cent of the sources in our sample show significant variations on hourly time-scales. Using the Mann-Whitney U-test and the Kolmogorov-Smirnov test, we find no difference in the variability behaviour between Seyfert 1 and 2 galaxies. The blazar sources (FSRQs and BL Lacs) in our sample are more variable than Seyfert galaxies that include Seyfert 1 and Seyfert 2 in the soft (3-10 keV), hard (10-79 keV) and total (3-79 keV) bands. NLSy1 galaxies show the highest duty cycle of variability (87 per cent), followed by BL Lacs (82 per cent), Seyfert galaxies (56 per cent) and FSRQs (23 per cent). We obtained flux doubling/halving time in the hard X-ray band less than 10 min in 11 sources. The flux variations between the hard and soft bands in all the sources in our sample are consistent with zero lag.

X-Ray Variability of BL Lac Objects

NASA Astrophysics Data System (ADS)

McHardy, Ian

I present an overview of the X-ray temporal and spectral variability of BL Lacs on both short and long timescales. The previously observed behaviour of short (~days) flares superimposed on a relatively steady `quiescent' level is still broadly correct. However, for the brighter BL Lacs, the well sampled lightcurves from the RXTE ASM show that the `quiescent' level also varies considerably on timescales of ~100 days in a manner similar to that seen in Optically Violently Variable Quasars (OVVs) such as 3C279 and 3C273. Possible reasons for this behaviour are discussed. For the large majority of BL Lacs the soft and medium energy X-ray bands are dominated by synchrotron emission and, unlike the case of OVVs, the emission mechanism is not in doubt. Most interest then centres on the structure of the emitting region, and the electron acceleration processes, particularly during outbursts. That structure, and the acceleration processes, can be investigated by consideration of the spectral variability during flares, which is not simple. I review the observations of spectral variability and consider the evidence for and against homogeneous models. I also briefly compare the X-ray spectral variability of BL Lacs with that of OVVs such as 3C273.

The Discovery of Low-Luminosity BL Lacs

NASA Astrophysics Data System (ADS)

Rector, Travis A.; Stocke, John T.

1995-12-01

Many of the properties of BL Lacs have become explicable in terms of the ``relativistic beaming'' hypothesis whereby BL Lacs are ``highly beamed'' FR-I radio galaxies (i.e. our line of sight to these objects is nearly along the jet axis). Further, radio-selected BL Lacs (RBLs) are believed to be seen nearly ``on-axis'' (the line-of-sight angle theta ~ 8deg ) while X-ray selected BL Lacs (XBLs) are seen at larger angles (theta ~ 30deg ; the X-ray emitting jet is believed to be less collimated). However, a major problem with this model was that a transition population between beamed BL Lacs and unbeamed FR-Is had not been detected. Low-luminosity BL Lacs may be such a transition population, and were predicted to exist by Browne and Marcha (1993). We present ROSAT HRI images, VLA radio maps and optical spectra which confirm the existence of low-luminosity BL Lacs, objects which were previously mis-identified in the EMSS catalog as clusters of galaxies. Thus our results strengthen the relativistic beaming hypothesis.

The REX survey as a Tool to Test the Beaming Model for BL Lacs

NASA Astrophysics Data System (ADS)

Caccianiga, A.; della Ceca, R.; Gioia, I. M.; Maccacaro, T.; Wolter, A.

We present the preliminary properties of the BL Lacs discovered in the REX survey (Caccianiga et al. 1998). In particular, we discuss a few sources with optical spectral properties ``intermediate'' between those of BL Lacs and those of elliptical galaxies. These objects could harbour weak (in the optical band) sources of non-thermal continuum in their nuclei and, if confirmed, they could represent the faint tail of the BL Lac population. The existence of such ``weak'' BL Lacs is matter of discussion in recent literature (e.g. Marcha et al. 1996) and could lead to a revision of the defining criteria of a BL Lac and, consequently, of their cosmological and statistical properties.

Photometric Redshifts of High-z BL Lacs from 3FGL Catalog

NASA Astrophysics Data System (ADS)

Kaur, A.; Rau, Arne; Ajello, Marco; Paliya, Vaidehi; Hartmann, Dieter; Greiner, Jochen; Bolmer, Jan; Schady, Patricia

2017-08-01

Determining redshifts for BL Lacertae (BL Lac) objects using the traditional spectroscopic method is challenging due to the absence of strong emission lines in their optical spectra. We employ the photometric dropout technique to determine redshifts for this class of blazars using the combined 13 broad-band filters from Swift-UVOT and the multi-channel imager GROND at the MPG 2.2 m telescope at ESO's La Silla Observatory. The wavelength range covered by these 13 filters extends from far ultraviolet to the near-Infrared. We report results on 40 new Fermi detected BL Lacs with the photometric redshifts determinations for 5 sources, with 3FGL J1918.2-4110 being the most distance in our sample at z=2.16. Reliable upper limits are provided for 20 sources in this sample. Using the highest energy photons for these Fermi-LAT sources, we evaluate the consistency with the Gamma-ray horizon due to the extragalactic background light.

The BL Lac Classification

NASA Astrophysics Data System (ADS)

Landt, H.; Padovani, P.

1999-12-01

In the optical wavelength range the distinction between a radio galaxy and a BL Lac object is mainly based on the Ca II H and K break observed in the optical spectrum. Marchã et al. (1996, MNRAS, 281, 425) have expanded on the previously used division by suggesting objects with Ca II break values lower than 0.4 to be classified as BL Lacs and sources with values higher than 0.4 to be classified as galaxies. We present new evidence that there is a smooth transition between BL Lac objects and Fanaroff-Riley type I radio galaxies. We find an increase in X-ray and radio core luminosity as the Ca II break gets more and more diluted. This suggests that the only difference between BL Lac objects and their parent population lies in orientation. The closer the jet of the radio galaxy to the observer's line of sight, the more its luminosity gets amplified and the object becomes BL Lac-like. We will address the question of the BL Lac parent population and will propose to unify the beamed and unbeamed objects in nomenclature.

An emerging population of BL Lacs with extreme properties: towards a class of EBL and cosmic magnetic field probes?

NASA Astrophysics Data System (ADS)

Bonnoli, G.; Tavecchio, F.; Ghisellini, G.; Sbarrato, T.

2015-07-01

High-energy observations of extreme BL Lac objects, such as 1ES 0229+200 or 1ES 0347-121, recently focused interest both for blazar and jet physics and for the implication on the extragalactic background light and intergalactic magnetic field estimate. However, the number of these extreme highly peaked BL Lac objects (EHBL) is still rather small. Aiming at increase their number, we selected a group of EHBL candidates starting from the BL Lac sample of Plotkin et al. (2011), considering those undetected (or only barely detected) by the Large Area Telescope onboard Fermi and characterized by a high X-ray versus radio flux ratio. We assembled the multiwavelength spectral energy distribution of the resulting nine sources, profiting of publicly available archival observations performed by Swift, GALEX, and Fermi satellites, confirming their nature. Through a simple one-zone synchrotron self-Compton model we estimate the expected very high energy flux, finding that in the majority of cases it is within the reach of present generation of Cherenkov arrays or of the forthcoming Cherenkov Telescope Array.

Stellar Populations in BL Lac type Objects

NASA Astrophysics Data System (ADS)

Serote Roos, Margarida

The relationship between an Active Galactic Nucleus (AGN) and its host galaxy is a crucial question in the study of galaxy evolution. We present an estimate of the stellar contribution in a sample of low luminosity BL Lac type objects. We have performed stellar population synthesis for a sample of 19 objects selected from Marchã et al. (1996, MNRAS 281, 425). The stellar content is quantified using the equivalent widths of all absorption features available throughout the spectrum. The synthesis is done by a variant of the GPG method (Pelat: 1997, MNRAS 284, 365).

Using the Markov chain Monte Carlo method to study the physical properties of GeV-TeV BL Lac objects

NASA Astrophysics Data System (ADS)

Qin, Longhua; Wang, Jiancheng; Yang, Chuyuan; Yuan, Zunli; Mao, Jirong; Kang, Shiju

2018-01-01

We fit the spectral energy distributions (SEDs) of 46 GeV-TeV BL Lac objects in the frame of leptonic one-zone synchrotron self-Compton (SSC) model and investigate the physical properties of these objects. We use the Markov chain Monte Carlo (MCMC) method to obtain the basic parameters, such as magnetic field (B), the break energy of the relativistic electron distribution (γ ^' }b), and the electron energy spectral index. Based on the modeling results, we support the following scenarios for GeV-TeV BL Lac objects. (1) Some sources have large Doppler factors, implying other radiation mechanism should be considered. (2) Compared with flat spectrum quasars (FSRQs), GeV-TeV BL Lac objects have weaker magnetic fields and larger Doppler factors, which cause the ineffective cooling and shift the SEDs to higher bands. Their jet powers are around 4.0 × 1045 erg s-1, compared with radiation power, 5.0 × 1042 erg s-1, indicating that only a small fraction of jet power is transformed into the emission power. (3) For some BL Lacs with large Doppler factors, their jet components could have two substructures, e.g., the fast core and the slow sheath. For most GeV-TeV BL Lacs, Kelvin-Helmholtz instabilities are suppressed by their higher magnetic fields, leading to micro-variability or intro-day variability in the optical bands. (4) Combined with a sample of FSRQs, an anti-correlation between the peak luminosity, Lpk, and the peak frequency, νpk, is obtained, favoring the blazar sequence scenario. In addition, an anti-correlation between the jet power, Pjet, and the break Lorentz factor, γb, also supports the blazar sequence.

Radio constraints on the nature of BL Lacertae objects and their parent population

NASA Technical Reports Server (NTRS)

Kollgaard, R. I.; Wardle, J. F. C.; Roberts, D. H.; Gabuzda, D. C.

1992-01-01

5 GHz VLA observations of 17 BL Lac objects with bright radio cores at both high and low resolution are reported. Extended emission is detected around most objects. None of the sources observed at low resolution show evidence of giant halos on the scale of tens of arcmin. In general, the sources with the most luminous extended emission exhibit FR II characteristics in both morphology and polarization, and less luminous sources exhibit FR I characteristics. Thus, the parent population of the BL Lac objects contains both FR I and FR II radio sources. No BL Lac objects are found that clearly exhibit quasarlike polarization at milliarcsec resolution. This argues against the view that the more luminous BL Lac objects are simply an extension of the quasar/OVV population, or that most BL Lac objects are gravitationally microlensed images of distant quasars. Other properties are generally consistent with the view the BL Lac objects are normal radio galaxies whose jets make a small angle to the line of sight.

Empirical multi-wavelength prediction method for very high energy gamma-ray emitting BL Lacertae objects

NASA Astrophysics Data System (ADS)

Fallah Ramazani, V.; Lindfors, E.; Nilsson, K.

2017-12-01

Aim: We have collected the most complete multi-wavelength (6.0-6.0 × 10-18 cm) dataset of very high energy (VHE) γ-ray emitting (TeV) BL Lacs, which are the most numerous extragalactic VHE sources. Using significant correlations between different bands, we aim to identify the best TeV BL Lac candidates that can be discovered by the current and next generation of imaging air Cherenkov telescopes. Methods: We formed five datasets from lower energy data, i.e. radio, mid-infrared, optical, X-rays, and GeV γ-ray, and five VHE γ-ray datasets to perform a correlation study between different bands and to construct the prediction method. The low energy datasets were averaged for individual sources, while the VHE γ-ray data were divided into subsets according to the flux state of the source. We then looked for significant correlations and determined their best-fit parameters. Using the best-fit parameters we predicted the level of VHE γ-ray flux for a sample of 182 BL Lacs, which have not been detected at TeV energies. We identified the most promising TeV BL Lac candidates based on the predicted VHE γ-ray flux for each source. Results: We found 14 significant correlations between radio, mid-infrared, optical, γ-ray, and VHE γ-ray bands. The correlation between optical and VHE γ-ray luminosity is established for the first time. We attribute this to the more complete sample and more accurate handling of host galaxy flux in our work. We found nine BL Lac candidates whose predicted VHE γ-ray flux is high enough for detection in less than 25 h with current imaging air Cherenkov telescopes. Full Tables A.1 and A.2 are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/608/A68

VizieR Online Data Catalog: Fermi/non-Fermi blazars jet power and accretion (Chen+, 2015)

NASA Astrophysics Data System (ADS)

Chen, Y. Y.; Zhang, X.; Zhang, H. J.; Yu, X. L.

2017-11-01

We selected the sample using radio catalogues to get the widest possible sample of blazars based on their radio properties. We split them into Fermi-detected sources and non-Fermi detections. Massaro et al. (2009, J/A+A/495/691) created the "Multifrequency Catalogue of Blazars" (Roma-BZCAT), which classifies blazars into three main groups based on their spectral properties. In total, we have a sample containing 177 clean Fermi blazars (96 Fermi FSRQs and 81 Fermi BL Lacs) and 133 non-Fermi blazars (105 non-Fermi FSRQs and 28 non-Fermi BL Lacs). (2 data files).

The third catalog of active galactic nuclei detected by the Fermi large area telescope

DOE PAGES

Ackermann, M.; Ajello, M.; Atwood, W. B.; ...

2015-08-25

We present the third catalog of active galactic nuclei (AGNs) detected by the Fermi-LAT (3LAC). It is based on the third Fermi-LAT catalog (3FGL) of sources detected between 100 MeV and 300 GeV with a Test Statistic greater than 25, between 2008 August 4 and 2012 July 31. The 3LAC includes 1591 AGNs located at high Galactic latitudes (more » $$| b| \\gt 10^\\circ $$), a 71% increase over the second catalog based on 2 years of data. There are 28 duplicate associations, thus 1563 of the 2192 high-latitude gamma-ray sources of the 3FGL catalog are AGNs. Most of them (98%) are blazars. About half of the newly detected blazars are of unknown type, i.e., they lack spectroscopic information of sufficient quality to determine the strength of their emission lines. Based on their gamma-ray spectral properties, these sources are evenly split between flat-spectrum radio quasars (FSRQs) and BL Lacs. The most abundant detected BL Lacs are of the high-synchrotron-peaked (HSP) type. There were about 50% of the BL Lacs that had no measured redshifts. A few new rare outliers (HSP-FSRQs and high-luminosity HSP BL Lacs) are reported. The general properties of the 3LAC sample confirm previous findings from earlier catalogs. The fraction of 3LAC blazars in the total population of blazars listed in BZCAT remains non-negligible even at the faint ends of the BZCAT-blazar radio, optical, and X-ray flux distributions, which hints that even the faintest known blazars could eventually shine in gamma-rays at LAT-detection levels. Furthermore, the energy-flux distributions of the different blazar populations are in good agreement with extrapolation from earlier catalogs.« less

Photometric monitoring of three BL Lacertae objects in 1993-1998

NASA Astrophysics Data System (ADS)

Bai, J. M.; Xie, G. Z.; Li, K. H.; Zhang, X.; Liu, W. W.

1999-05-01

The results of optical photometric (BVRI) monitoring of three BL Lac objects over a time interval of about four years are presented. The sources are three classical radio-selected BL Lac objects, BL Lac, OJ 287 and PKS 0735+178. During our observation OJ 287 was in the stage of a large periodic outburst which consisted of at least two peaks. Almost all the observations obtained over consecutive nights detected intranight variations. In 1995 and 1996 BL Lac kept in faint states, with fewer and smaller rapid flares and fluctuations. On the contrary, in late 1997 BL Lac was at the stage of a large outburst, accompanied with much more large amplitude rapid flares and fluctuations. PKS 0735+178 was almost at its faint end from 1994 to early 1998. Over this time interval, the intraday variations and microvariations in PKS 0735+178 were rare and the amplitude was very small, except a rapid darkening of ~ 0.4 mag on 24 January 1995. Previous work by \\cite[Webb et al. (1988);]{web88} \\cite[Wagner et al. (1996);]{wag96} \\cite[Pian et al. (1997)]{pia97} also showed the same behaviour of variability as BL Lac and PKS 0735+178 in BL Lac, S5 0716+714, PKS 2155-304, respectively. We propose that the motion of orientation of the relativistic jet in a BL Lac object be responsible for these variability behaviours. Table~1 is only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/Abstract.html

Active galaxies observed during the Extreme Ultraviolet Explorer all-sky survey

NASA Technical Reports Server (NTRS)

Marshall, H. L.; Fruscione, A.; Carone, T. E.

1995-01-01

We present observations of active galactic nuclei (AGNs) obtained with the Extreme Ultraviolet Explorer (EUVE) during the all-sky survey. A total of 13 sources were detected at a significance of 2.5 sigma or better: seven Seyfert galaxies, five BL Lac objects, and one quasar. The fraction of BL Lac objects is higher in our sample than in hard X-ray surveys but is consistent with the soft X-ray Einstein Slew Survey, indicating that the main reason for the large number of BL Lac objects in the extreme ulktraviolet (EUV) and soft X-ray bands is their steeper X-ray spectra. We show that the number of AGNs observed in both the EUVE and ROSAT Wide Field Camera surveys can readily be explained by modelling the EUV spectra with a simple power law in the case of BL Lac objects and with an additional EUV excess in the case of Seyferts and quasars. Allowing for cold matter absorption in Seyfert galaxy hosts drive up the inferred average continuum slope to 2.0 +/- 0.5 (at 90% confidence), compared to a slope of 1.0 usually found from soft X-ray data. If Seyfert galaxies without EUV excesses form a significant fraction of the population, then the average spectrum of those with bumps should be even steeper. We place a conservative limit on neutral gas in BL Lac objects: N(sub H) less than 10(exp 20)/sq cm.

AGN jets under the microscope: A divide? Doctoral Thesis Award Lecture 2011

NASA Astrophysics Data System (ADS)

Karouzos, M.; Britzen, S.; Witzel, A.; Zensus, A. J.; Eckart, A.

2012-06-01

A new paradigm for active galactic jet kinematics has emerged through detailed investigations of BL Lac objects using very long baseline radio interferometry. In this new scheme, most, if not all, jet components appear to remain stationary with respect to the core but show significant non-radial motions. This paper presents results from our kinematic investigation of the jets of a statistically complete sample of radio-loud flat-spectrum active galaxies, focusing on the comparison between the jet kinematic properties of BL Lacs and flat-spectrum radio-quasars. It is shown that there is a statistically significant difference between the kinematics of the two AGN classes, with BL Lacs showing more bent jets, that are wider and show slower movement along the jet axis, compared to flat-spectrum radio-quasars. This is interpreted as evidence for helically structured jets.

The intraday variability in the radio-selected and X-ray-selected BL Lacertae objects

NASA Astrophysics Data System (ADS)

Bai, J. M.; Xie, G. Z.; Li, K. H.; Zhang, X.; Liu, W. W.

1998-10-01

Seven BL Lac objects have been photometrically observed in an effort to study the difference of optical intraday variability between the radio-selected BL Lac objects (RBLs) and X-ray-selected BL Lac objects (XBLs). The objects we observed are selected arbitrarily. They are four RBLs, PKS 0735+178, PKS 0754+101, OJ 287 and BL Lac, and three XBLs, H 0323+022, H 0548-322 and H 2154-304. During the observation all of them exhibited microvariation, and H 0323+022 and H 0548-322 sometimes showed brightness oscillation. PKS 0735+178 and BL Lac were in their faint states and not very active. It seems that RBLs do not show microvariability more frequently than XBLs. Table 2 is only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5)

Are some BL Lacs artefacts of gravitational lensing?

PubMed

Ostriker, J P; Vietri, M

1990-03-01

WE suggested in 1985 that a significant fraction of BL Lacertae objects, a kind of lineless quasar, seen in nearby galaxies are in fact images, gravitationally lensed and substantially amplified by stars in the nearby galaxy, of background objects, optically violent variable (OVV) quasars at redshifts z > 1 (ref. 1). This hypothesis was made on the basis of certain general similarities between BL Lacs and O Ws, but for two recently observed BL Lacs(2,3) a strong case can be made that the accompanying elliptical galaxy is a foreground object. In addition, we argue that the distribution of BL Lac redshifts is hard to understand without gravitational lensing, unless we happen to be at a very local maximum of the spatial cosmic distribution of BL Lacs. Our analysis also indicates that the galaxies whose stars are likely to act as microlenses will be found in two peaks, one nearby, with redshift 0.05-0.10, and the other near the distant quasar.

An Analysis of Light Periods of BL Lac Object S5 0716+714 with the MUSIC Algorithm

NASA Astrophysics Data System (ADS)

Tang, Jie

2012-07-01

The multiple signal classification (MUSIC) algorithm is introduced to the estimation of light periods of BL Lac objects. The principle of the MUSIC algorithm is given, together with a testing on its spectral resolution by using a simulative signal. From a lot of literature, we have collected a large number of effective observational data of the BL Lac object S5 0716+714 in the three optical wavebands V, R, and I from 1994 to 2008. The light periods of S5 0716+714 are obtained by means of the MUSIC algorithm and average periodogram algorithm, respectively. It is found that there exist two major periodic components, one is the period of (3.33±0.08) yr, another is the period of (1.24±0.01) yr. The comparison of the performances of periodicity analysis of two algorithms indicates that the MUSIC algorithm has a smaller requirement on the sample length, as well as a good spectral resolution and anti-noise ability, to improve the accuracy of periodicity analysis in the case of short sample length.

Effective spectral index properties for Fermi blazars

NASA Astrophysics Data System (ADS)

Yang, JiangHe; Fan, JunHui; Liu, Yi; Zhang, YueLian; Tuo, ManXian; Nie, JianJun; Yuan, YuHai

2018-05-01

Blazars are a special subclass of active galactic nuclei with extreme observation properties. This subclass can be divided into two further subclasses of flat spectrum radio quasars (FSRQs) and BL Lacertae objects (BL Lacs) according to their emission line features. To compare the spectral properties of FSRQs and BL Lacs, the 1.4 GHz radio, optical R-band, 1 keV X-ray, and 1 GeV γ-ray flux densities for 1108 Fermi blazars are calculated to discuss the properties of the six effective spectral indices of radio to optical ( α RO), radio to X-ray ( α RX), radio to γ ray ( α Rγ), optical to X-ray ( α OX), optical to γ ray ( α Oγ), and X-ray to γ ray ( α Xγ). The main results are as follows: For the averaged effective spectral indices, \\overline {{α _{OX}}} > \\overline {{α _{Oγ }}} > \\overline {{α _{Xγ }}} > \\overline {{α _{Rγ }}} > \\overline {{α _{RX}}} > \\overline {{α _{RO}}} for samples of whole blazars and BL Lacs; \\overline {{α _{Xγ }}} ≈ \\overline {{α _{Rγ }}} ≈ \\overline {{α _{RX}}} for FSRQs and low-frequency-peaked BL Lacs (LBLs); and \\overline {{α _{OX}}} ≈ \\overline {{α _{Oγ }}} ≈ \\overline {{α _{Xγ }}} for high-synchrotron-frequency-peaked BL Lacs (HBLs). The distributions of the effective spectral indices involving optical emission ( α RO, α OX, and α Oγ) for LBLs are different from those for FSRQs, but if the effective spectral index does not involve optical emission ( α RX, α Rγ, and α Xγ), the distributions for LBLs and FSRQs almost come from the same parent population. X-ray emissions from blazars include both synchrotron and inverse Compton (IC) components; the IC component for FSRQs and LBLs accounts for a larger proportion than that for HBLs; and the radiation mechanism for LBLs is similar to that for FSRQs, but the radiation mechanism for HBLs is different from that for both FSRQs and LBLs in X-ray bands. The tendency of α Rγ decreasing from LBLs to HBLs suggests that the synchrotron self-Compton model explains the main process for highly energetic γ rays in BL Lacs.

Are some BL Lac objects artefacts of gravitational lensing?

NASA Technical Reports Server (NTRS)

Ostriker, J. P.; Vietri, M.

1985-01-01

It is proposed here that a significant fraction of BL Lac objects are optically violently variable quasars whose continuum emission has been greatly amplified, relative to the line emission, by pointlike gravitational lenses in intervening galaxies. Several anomalous physical and statistical properties of BL Lacs can be understood on the basis of this model, which is immediately testable on the basis of absorption line studies and by direct imaging.

Clustering environments of BL Lac objects

NASA Technical Reports Server (NTRS)

Wurtz, Ronald; Ellingson, Erica; Stocke, John T.; Yee, H. K. C.

1993-01-01

We report measurements of the amplitude of the BL Lac galaxy spatial covariance function, B(gb), for the fields of five BL Lacertae objects. We present evidence for rich clusters around MS 1207+39 and MS 1407+59, and confirm high richness for the cluster containing H0414+009. We discuss the ease of 3C 66 A and find evidence for a poor cluster based on an uncertain redshift of z = 0.444. These data suggest that at least some BL Lac objects are consistent with being FR 1 radio galaxies in rich clusters.

Characteristics of Gamma-Ray Loud Blazars in the VLBA Imaging and Polarimetry Survey

NASA Technical Reports Server (NTRS)

Linford, J. D.; Taylor, G. B.; Romani, R. W.; Healey, S. E.; Helmboldt, J. F.; Readhead, A. C.; Reeves, R.; Richards, J. L.; Cotter, G.

2010-01-01

The radio properties of blazars detected by the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope have been observed as part of the VLBA Imaging and Polarimetry Survey. This large, flux-limited sample of active galactic nuclei (AGNs) provides insights into the mechanism that produces strong gamma-ray emission. At lower flux levels, radio flux density does not directly correlate with gamma-ray flux. We find that the LAT-detected BL Lac objects tend to be similar to the non-LAT BL Lac objects, but that the LAT-detected FSRQs are often significantly different from the non-LAT FSRQs. The differences between the gamma-ray loud and quiet FSRQS can be explained by Doppler boosting; these objects appear to require larger Doppler factors than those of the BL Lac objects. It is possible that the gamma-ray loud FSRQs are fundamentally different from the gamma-ray quiet FSRQs. Strong polarization at the base of the jet appears to be a signature for gamma-ray loud AGNs.

VLBA Observations of Low Luminosity Flat Spectrum Radio Galaxies and BL Lac Objects: Polarisation Properties

NASA Astrophysics Data System (ADS)

Bondi, M.; Dallacasa, D.; Stanghellini, C.; Marchã, M. J. M.

We obtained two-epoch VLBA observations at 5 GHz of a list of radio galaxies drawn from the 200 mJy sample (Marcha et al. 1996). The objects selected for milli-arcsecond scale observations are classified, on the basis of their optical spectroscopic and polarimetric properties, as BL Lac objects, normal weak line radio galaxies, broad line radio galaxies, and transition objects (those with intermediate properties). We present preliminary results on the radio polarization properties, on the milli-arcsecond scale, of objects with different optical properties and discuss structural variations detected from the two epochs.

The BL LAC phenomenon: X-ray observations of transition objects and determination of the x-ray spectrum of a complete sample of flat-spectrum radio sources

NASA Technical Reports Server (NTRS)

Worrall, Diana M.

1994-01-01

This report summarizes the activities related to two ROSAT investigations: (1) x-ray properties of radio galaxies thought to contain BL Lac type nuclei; and (2) x-ray spectra of a complete sample of flat-spectrum radio sources. The following papers describing the research are provided as attachments: Multiple X-ray Emission Components in Low Power Radio Galaxies; New X-ray Results on Radio Galaxies; Analysis Techniques for a Multiwavelength Study of Radio Galaxies; Separation of X-ray Emission Components in Radio Galaxies; X-ray Emission in Powerful Radio Galaxies and Quasars; Extended and Compact X-ray Emission in Powerful Radio Galaxies; and X-ray Spectra of a Complete Sample of Extragalactic Core-dominated Radio Sources.

On the surface density of X-ray selected BL Lacertae objects

NASA Technical Reports Server (NTRS)

Maccacaro, T.; Gioia, I. M.; Maccagni, D.; Stocke, J. T.

1984-01-01

Only a handful of BL Lac objects have been found as a result of systematic optical identification of serendipitous Einstein X-ray sources. By combining the data from two flux-limited complete X-ray surveys (the HEAO 1 A-2 and the Einstein Observatory Medium Sensitivity Survey) the surface density of X-ray emitting BL Lac objects is evaluated as a function of their X-ray flux. It is found that a single power law is not an acceptable representation of the BL Lac objects' X-ray log N-log S. The number-flux relationship is consistent with the Euclidean slope at 'high' flux levels but shows a drastic flattnring below fluxes of the order of 10 to the -12th ergs per sq cm/s. The implications of this result are briefly discussed with respect to the luminosity function, the cosmological evolution, and the X-ray to optical flux ratio in BL Lac objects.

Low-luminosity Blazars in Wise: A Mid-infrared View of Unification

NASA Astrophysics Data System (ADS)

Plotkin, Richard M.; Anderson, S. F.; Brandt, W. N.; Markoff, S.; Shemmer, O.; Wu, J.

2012-01-01

We use the preliminary data release from the Wide-Field Infrared Survey Explorer (WISE) to perform the first statistical study on the mid-infrared (IR) properties of a large number ( 102) of BL Lac objects -- low-luminosity Active Galactic Nuclei (AGN) with a jet beamed toward the Earth. As expected, many BL Lac objects are so highly beamed that their jet synchrotron emission dominates their IR spectral energy distributions (SEDs), and the shape of their SEDs in the IR correlates well with SED peak frequency. In other BL Lac objects, the jet is not strong enough to completely dilute the rest of the AGN, and we do not see observational signatures of the dusty torus from these weakly beamed BL Lac objects. While at odds with simple unification, the missing torus is consistent with recent suggestions that BL Lac objects are fed by radiatively inefficient accretion flows. We discuss implications on the ``nature vs. nurture" debate for FR I and FR II galaxies, and also on the standard orientation-based AGN unification model.

The Second Catalog Of Active Galactic Nuclei Detected By The Fermi Large Area Telescope

DOE PAGES

Ackermann, M.

2011-12-02

The second catalog of active galactic nuclei (AGNs) detected by the Fermi Large Area Telescope (LAT) in two years of scientific operation is presented. The Second LAT AGN Catalog (2LAC) includes 1017 γ-ray sources located at high Galactic latitudes (|b| > 10°) that are detected with a test statistic (TS) greater than 25 and associated statistically with AGNs. However some of these are affected by analysis issues and some are associated with multiple AGNs. Consequently we define a clean sample which includes 886 AGNs, comprising 395 BL Lacertae objects (BL Lacs), 310 flat-spectrum radio quasars (FSRQs), 157 candidate blazars ofmore » unknown type (i.e., with broad-band blazar characteristics but with no optical spectral measurement yet), eight misaligned AGNs, four narrow-line Seyfert 1 (NLS1s), 10 AGNs of other types and two starburst galaxies. Where possible, the blazars have been further classified based on their spectral energy distributions (SEDs) as archival radio, optical, and X-ray data permit. While almost all FSRQs have a synchrotron-peak frequency < 10 14 Hz, about half of the BL Lacs have a synchrotron-peak frequency > 10 15 Hz. The 2LAC represents a significant improvement relative to the First LAT AGN Catalog (1LAC), with 52% more associated sources. The full characterization of the newly detected sources will require more broad-band data. Various properties, such as γ-ray fluxes and photon power law spectral indices, redshifts, γ-ray luminosities, variability, and archival radio luminosities—and their correlations are presented and discussed for the different blazar classes. The general trends observed in 1LAC are confirmed.« less

THE SECOND CATALOG OF ACTIVE GALACTIC NUCLEI DETECTED BY THE FERMI LARGE AREA TELESCOPE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ackermann, M.; Ajello, M.; Allafort, A.

The second catalog of active galactic nuclei (AGNs) detected by the Fermi Large Area Telescope (LAT) in two years of scientific operation is presented. The second LAT AGN catalog (2LAC) includes 1017 {gamma}-ray sources located at high Galactic latitudes (|b| > 10 Degree-Sign ) that are detected with a test statistic (TS) greater than 25 and associated statistically with AGNs. However, some of these are affected by analysis issues and some are associated with multiple AGNs. Consequently, we define a Clean Sample which includes 886 AGNs, comprising 395 BL Lacertae objects (BL Lac objects), 310 flat-spectrum radio quasars (FSRQs), 157more » candidate blazars of unknown type (i.e., with broadband blazar characteristics but with no optical spectral measurement yet), 8 misaligned AGNs, 4 narrow-line Seyfert 1 (NLS1s), 10 AGNs of other types, and 2 starburst galaxies. Where possible, the blazars have been further classified based on their spectral energy distributions (SEDs) as archival radio, optical, and X-ray data permit. While almost all FSRQs have a synchrotron-peak frequency <10{sup 14} Hz, about half of the BL Lac objects have a synchrotron-peak frequency >10{sup 15} Hz. The 2LAC represents a significant improvement relative to the first LAT AGN catalog (1LAC), with 52% more associated sources. The full characterization of the newly detected sources will require more broadband data. Various properties, such as {gamma}-ray fluxes and photon power-law spectral indices, redshifts, {gamma}-ray luminosities, variability, and archival radio luminosities and their correlations are presented and discussed for the different blazar classes. The general trends observed in 1LAC are confirmed.« less

Optical polarization of high-energy BL Lacertae objects

NASA Astrophysics Data System (ADS)

Hovatta, T.; Lindfors, E.; Blinov, D.; Pavlidou, V.; Nilsson, K.; Kiehlmann, S.; Angelakis, E.; Fallah Ramazani, V.; Liodakis, I.; Myserlis, I.; Panopoulou, G. V.; Pursimo, T.

2016-12-01

Context. We investigate the optical polarization properties of high-energy BL Lac objects using data from the RoboPol blazar monitoring program and the Nordic Optical Telescope. Aims: We wish to understand if there are differences between the BL Lac objects that have been detected with the current-generation TeV instruments and those objects that have not yet been detected. Methods: We used a maximum-likelihood method to investigate the optical polarization fraction and its variability in these sources. In order to study the polarization position angle variability, we calculated the time derivative of the electric vector position angle (EVPA) change. We also studied the spread in the Stokes Q/I-U/I plane and rotations in the polarization plane. Results: The mean polarization fraction of the TeV-detected BL Lacs is 5%, while the non-TeV sources show a higher mean polarization fraction of 7%. This difference in polarization fraction disappears when the dilution by the unpolarized light of the host galaxy is accounted for. The TeV sources show somewhat lower fractional polarization variability amplitudes than the non-TeV sources. Also the fraction of sources with a smaller spread in the Q/I-U/I plane and a clumped distribution of points away from the origin, possibly indicating a preferred polarization angle, is larger in the TeV than in the non-TeV sources. These differences between TeV and non-TeV samples seem to arise from differences between intermediate and high spectral peaking sources instead of the TeV detection. When the EVPA variations are studied, the rate of EVPA change is similar in both samples. We detect significant EVPA rotations in both TeV and non-TeV sources, showing that rotations can occur in high spectral peaking BL Lac objects when the monitoring cadence is dense enough. Our simulations show that we cannot exclude a random walk origin for these rotations. Conclusions: These results indicate that there are no intrinsic differences in the polarization properties of the TeV-detected and non-TeV-detected high-energy BL Lac objects. This suggests that the polarization properties are not directly related to the TeV-detection, but instead the TeV loudness is connected to the general flaring activity, redshift, and the synchrotron peak location. The polarization curve data are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/596/A78

Magnetic Fields in Blazar Jets: Radio and Optical Polarization over 20-30 Years

NASA Astrophysics Data System (ADS)

Caldwell, Caroline; Wills, B.; Wills, D.; Aller, H.; Aller, M.

2011-01-01

Blazars are highly active nuclei of distant galaxies. They produce synchrotron-emitting relativistic jets on scales of less than a parsec to many Kpc. When viewed head-on, as opposed to in the plane of the sky, the jet motion appears superluminal, and the emission is Doppler boosted. Blazars show rapid radio and optical variability in flux density and polarization. There are two types of blazars that can have strong synchrotron continua: non-BL Lac blazars with strong broad emission lines (quasars), and BL Lac objects with only weak lines. We have compiled optical linear polarization measurements of 22 blazars, incorporating much archival data from McDonald Observatory. While the optical data are somewhat sparsely sampled, The University of Michigan Radio Astronomical Observatory observed many blazars over 20-30 years, often well-sampled over days to weeks. These data enabled us to compare optical and radio polarization position angles. We constructed histograms of the separation of polarization position angles of the optical and radio. We found that in BL Lac objects, the histogram has a significant peak at zero separation. Since the polarization position angle indicates the direction perpendicular to the magnetic field vector, finding similar polarization position angles indicates a similar magnetic field at the origin of the optical and radio synchrotron radiation. Non-BL Lac blazars show peaks at zero and 90 degree separation of position angle. The 90 degree separation may be caused by optical depth effects within the jet. Although there are a few sources that do not strongly display the characteristics summarized by the histograms, most sources produce optical and radio polarization position angles that nearly coincide or are separated by 90 degrees. Using VLBA and VLA radio maps, we interpret the results in terms of the position angle of the jet in the sky plane.

Fermi LAT detection of a GeV flare from the BL Lac object PKS 2233-148

NASA Astrophysics Data System (ADS)

Ciprini, Stefano

2012-06-01

The Large Area Telescope (LAT), one of the two instruments on the Fermi Gamma-ray Space Telescope, has observed gamma-ray flaring activity from a source positionally consistent with the BL Lac object PKS 2233-148 (also known as 2FGL J2236.5-1431, Nolan et al. 2012, ApJS, 199, 31, and OY -156) placed at R.A.: 339.1420296 deg, Dec.: -14.5561633 (J2000, Petrov et al. 2008, AJ, 136, 580). No redshift for the source has been measured up to now, demonstrating the BL Lac object character type of this source.

RADIO-WEAK BL LAC OBJECTS IN THE FERMI ERA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Massaro, F.; Marchesini, E. J.; D’Abrusco, R.

2017-01-10

The existence of “radio-weak BL Lac objects” (RWBLs) has been an open question, and has remained unsolved since the discovery that quasars could be radio-quiet or radio-loud. Recently, several groups identified RWBL candidates, mostly found while searching for low-energy counterparts of the unidentified or unassociated gamma-ray sources listed in the Fermi catalogs. Confirming RWBLs is a challenging task since they could be confused with white dwarfs (WDs) or weak emission line quasars (WELQs) when there are not sufficient data to precisely draw their broadband spectral energy distribution, and their classification is mainly based on a featureless optical spectra. Motivated bymore » the recent discovery that Fermi BL Lacs appear to have very peculiar mid-IR emission, we show that it is possible to distinguish between WDs, WELQs, and BL Lacs using the [3.4]–[4.6]–[12] μ m color–color plot built using the WISE magnitudes when the optical spectrum is available. On the basis of this analysis, we identify WISE J064459.38+603131 and WISE J141046.00+740511.2 as the first two genuine RWBLs, both potentially associated with Fermi sources. Finally, to strengthen our identification of these objects as true RWBLs, we present multifrequency observations for these two candidates to show that their spectral behavior is indeed consistent with that of the BL Lac population.« less

Radio-weak BL Lac Objects in the Fermi Era

NASA Astrophysics Data System (ADS)

Massaro, F.; Marchesini, E. J.; D'Abrusco, R.; Masetti, N.; Andruchow, I.; Smith, Howard A.

2017-01-01

The existence of “radio-weak BL Lac objects” (RWBLs) has been an open question, and has remained unsolved since the discovery that quasars could be radio-quiet or radio-loud. Recently, several groups identified RWBL candidates, mostly found while searching for low-energy counterparts of the unidentified or unassociated gamma-ray sources listed in the Fermi catalogs. Confirming RWBLs is a challenging task since they could be confused with white dwarfs (WDs) or weak emission line quasars (WELQs) when there are not sufficient data to precisely draw their broadband spectral energy distribution, and their classification is mainly based on a featureless optical spectra. Motivated by the recent discovery that Fermi BL Lacs appear to have very peculiar mid-IR emission, we show that it is possible to distinguish between WDs, WELQs, and BL Lacs using the [3.4]-[4.6]-[12] μm color-color plot built using the WISE magnitudes when the optical spectrum is available. On the basis of this analysis, we identify WISE J064459.38+603131 and WISE J141046.00+740511.2 as the first two genuine RWBLs, both potentially associated with Fermi sources. Finally, to strengthen our identification of these objects as true RWBLs, we present multifrequency observations for these two candidates to show that their spectral behavior is indeed consistent with that of the BL Lac population.

Jet Power vs. Black Hole Mass in Blazars: Exploring the Relationship in the Context of the B-Z Mechanism

NASA Astrophysics Data System (ADS)

Fernandes, Sunil; Schlegel, E.

2012-01-01

Recently, a tentative negative correlation between jet power and BH mass in a sample of GeV-TeV BL Lac objects(Zhang et al 2011). It was suggested that spin energy extraction could play a significant role in producing the jets and the jets are not purely accretion driven. Broderick et al (2011) recently explored the relationship between jet power and radio core luminosity building on Blanford et al (1979) theoretical work. Using this work we have studied the relationship between radio core luminosity (as a stand in for jet power) and black hole mass and have found a possible positive correlation in a sample of nearby BL Lac objects. The present poster attempts to explore this relationship in the context of the Blanford-Znajek mechanism which predicts jet power increases with black hole mass, spin rate, and accretion rate.

The Evolution of Swift/BAT blazars and the origin of the MeV background

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ajello, M.; /SLAC /KIPAC, Menlo Park; Costamante, L.

2009-10-17

We use 3 years of data from the Swift/BAT survey to select a complete sample of X-ray blazars above 15 keV. This sample comprises 26 Flat-Spectrum Radio Quasars (FSRQs) and 12 BL Lac objects detected over a redshift range of 0.03 < z < 4.0. We use this sample to determine, for the first time in the 15-55 keV band, the evolution of blazars. We find that, contrary to the Seyfert-like AGNs detected by BAT, the population of blazars shows strong positive evolution. This evolution is comparable to the evolution of luminous optical QSOs and luminous X-ray selected AGNs. Wemore » also find evidence for an epoch-dependence of the evolution as determined previously for radio-quiet AGNs. We interpret both these findings as a strong link between accretion and jet activity. In our sample, the FSRQs evolve strongly, while our best-fit shows that BL Lacs might not evolve at all. The blazar population accounts for 10-20% (depending on the evolution of the BL Lacs) of the Cosmic X-ray background (CXB) in the 15-55 keV band. We find that FSRQs can explain the entire CXB emission for energies above 500 keV solving the mystery of the generation of the MeV background. The evolution of luminous FSRQs shows a peak in redshift (z{sub c} = 4.3 {+-} 0.5) which is larger than the one observed in QSOs and X-ray selected AGNs. We argue that FSRQs can be used as tracers of massive elliptical galaxies in the early Universe.« less

STRUCTURED JETS IN BL LAC OBJECTS: EFFICIENT PeV NEUTRINO FACTORIES?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tavecchio, Fabrizio; Ghisellini, Gabriele; Guetta, Dafne

2014-09-20

The origin of high-energy neutrinos (0.1–1 PeV range) detected by IceCube remains a mystery. In this work, we explore the possibility that efficient neutrino production can occur in structured jets of BL Lac objects, characterized by a fast inner spine surrounded by a slower layer. This scenario has been widely discussed in the framework of the high-energy emission models for BL Lac objects and radio galaxies. One of the relevant consequences of a velocity structure is the enhancement of the inverse Compton emission caused by the radiative coupling of the two zones. We show that a similar boosting could occurmore » for the neutrino output of the spine through the photo-meson reaction of high-energy protons scattering off the amplified soft target photon field of the layer. Assuming the local density and the cosmological evolution of γ-ray BL Lac object derived from Fermi Large Area Telescope data, we calculate the expected diffuse neutrino intensity, which can match the IceCube data for a reasonable choice of parameters.« less

The Micro-Arcsecond Scintillation-Induced Variability (MASIV) Survey III. Optical Identifications and New Redshifts

NASA Technical Reports Server (NTRS)

Pursimo, Tapio; Ojha, Roopesh; Jauncey, David L.; Rickett, Barney J.; Dutka, Michael S.; Koay, Jun Yi; Lovell, James E. J.; Bignall, Hayley E.; Kedziora-Chudczer, Lucyna; Macquart, Jean-Pierre

2013-01-01

Intraday variability (IDV) of the radio emission from active galactic nuclei is now known to be predominantly due to interstellar scintillation (ISS). The MASIV (The Microarcsecond Scintillation Induced Variability) survey of 443 at spectrum sources revealed that the IDV is related to the radio flux density and redshift. A study of the physical properties of these sources has been severely handicapped by the absence of reliable redshift measurements for many of these objects. This paper presents 79 new redshifts and a critical evaluation of 233 redshifts obtained from the literature. We classify spectroscopic identifications based on emission line properties, finding that 78% of the sources have broad emission lines and are mainly FSRQs. About 16% are weak lined objects, chiefly BL Lacs, and the remaining 6% are narrow line objects. The gross properties (redshift, spectroscopic class) of the MASIV sample are similar to those of other blazar surveys. However, the extreme compactness implied by ISS favors FSRQs and BL Lacs in the MASIV sample as these are the most compact object classes. We confirm that the level of IDV depends on the 5 GHz flux density for all optical spectral types. We find that BL Lac objects tend to be more variable than broad line quasars. The level of ISS decreases substantially above a redshift of about two. The decrease is found to be generally consistent with ISS expected for beamed emission from a jet that is limited to a fixed maximum brightness temperature in the source rest frame.

Very high energy observations of the BL Lac objects 3C 66A and OJ 287

NASA Astrophysics Data System (ADS)

Lindner, T.; Hanna, D. S.; Kildea, J.; Ball, J.; Bramel, D. A.; Carson, J.; Covault, C. E.; Driscoll, D.; Fortin, P.; Gingrich, D. M.; Jarvis, A.; Mueller, C.; Mukherjee, R.; Ong, R. A.; Ragan, K.; Scalzo, R. A.; Williams, D. A.; Zweerink, J.

2007-11-01

Using the Solar Tower Atmospheric Cherenkov Effect Experiment (STACEE), we have observed the BL Lac objects 3C 66A and OJ 287. These are members of the class of low-frequency-peaked BL Lac objects (LBLs) and are two of the three LBLs predicted by Costamante and Ghisellini [L. Costamante, G. Ghisellini, Astron. Astrophys. 384 (2002) 56] to be potential sources of very high energy (>100 GeV) gamma-ray emission. The third candidate, BL Lacertae, has recently been detected by the MAGIC collaboration [J. Albert et al., arXiv:astro-ph/0703084v1 (2007)]. Our observations have not produced detections; we calculate a 99% CL upper limit of flux from 3C 66A of 0.15 Crab flux units and from OJ 287 our limit is 0.52 Crab. These limits assume a Crab-like energy spectrum with an effective energy threshold of 185 GeV.

X-Ray Spectral Variability Signatures of Flares in BL Lac Objects

NASA Technical Reports Server (NTRS)

Boettcher, Markus; Chiang, James; White, Nicholas E. (Technical Monitor)

2002-01-01

We are presenting a detailed parameter study of the time-dependent electron injection and kinematics and the self-consistent radiation transport in jets of intermediate and low-frequency peaked BL Lac objects. Using a time-dependent, combined synchrotron-self-Compton and external-Compton jet model, we study the influence of variations of several essential model parameters, such as the electron injection compactness, the relative contribution of synchrotron to external soft photons to the soft photon compactness, the electron- injection spectral index, and the details of the time profiles of the electron injection episodes giving rise to flaring activity. In the analysis of our results, we focus on the expected X-ray spectral variability signatures in a region of parameter space particularly well suited to reproduce the broadband spectral energy distributions of intermediate and low-frequency peaked BL Lac objects. We demonstrate that SSC- and external-Compton dominated models for the gamma-ray emission from blazars are producing significantly different signatures in the X-ray variability, in particular in the soft X-ray light curves and the spectral hysteresis at soft X-ray energies, which can be used as a powerful diagnostic to unveil the nature of the high-energy emission from BL Lac objects.

Parsec-Scale Kinematic and Polarization Properties of MOJAVE AGN Jets

NASA Astrophysics Data System (ADS)

Lister, Matthew L.

2013-12-01

We describe the parsec-scale kinematics and statistical polarization properties of 200 AGN jets based on 15 GHz VLBA data obtained between 1994 Aug 31 and 2011 May 1. Nearly all of the 60 most heavily observed jets show significant changes in their innermost position angle over a 12 to 16 year interval, ranging from 10° to 150° on the sky, corresponding to intrinsic variations of ~ 0.5° to ~ 2°. The BL Lac jets show smaller variations than quasars. Roughly half of the heavily observed jets show systematic position angle trends with time, and 20 show indications of oscillatory behavior. The time spans of the data sets are too short compared to the fitted periods (5 to 12 y), however, to reliably establish periodicity. The rapid changes and large jumps in position angle seen in many cases suggest that the superluminal AGN jet features occupy only a portion of the entire jet cross section, and may be energized portions of thin instability structures within the jet. We have derived vector proper motions for 887 moving features in 200 jets having at least five VLBA epochs. For 557 well-sampled features, there are sufficient data to additionally study possible accelerations. The moving features are generally non-ballistic, with 70% of the well-sampled features showing either significant accelerations or non-radial motions. Inward motions are rare (2% of all features), are slow (< 0.1 mas per y), are more prevalent in BL Lac jets, and are typically found within 1 mas of the unresolved core feature. There is a general trend of increasing apparent speed with distance down the jet for both radio galaxies and BL Lac objects. In most jets, the speeds of the features cluster around a characteristic value, yet there is a considerable dispersion in the distribution. Orientation variations within the jet cannot fully account for the dispersion, implying that the features have a range of Lorentz factor and/or pattern speed. Very slow pattern speed features are rare, comprising only 4% of the sample, and are more prevalent in radio galaxy and BL Lac jets. We confirm a previously reported upper envelope to the distribution of speed versus beamed luminosity for moving jet features. Below 1026 W Hz-1 there is a fall-off in maximum speed with decreasing 15 GHz radio luminosity. A preliminary analysis of the multi-epoch jet polarization properties indicates a wide range of behavior in the core electric vector position angles over time, with the latter remaining relatively stable in some jets, and varying rapidly in others. The fractional polarization level generally increases down the jet, and high-synchrotron peaked (HSP) blazars tend to have lower core fractional polarization levels. A general trend of decreasing maximum jet speed for higher synchrotron peaked blazars further suggests lower Doppler factors in the radio-emitting jets of HSP BL Lac objects.

THE MICRO-ARCSECOND SCINTILLATION-INDUCED VARIABILITY (MASIV) SURVEY. III. OPTICAL IDENTIFICATIONS AND NEW REDSHIFTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pursimo, Tapio; Ojha, Roopesh; Jauncey, David L.

2013-04-10

Intraday variability (IDV) of the radio emission from active galactic nuclei is now known to be predominantly due to interstellar scintillation (ISS). The MASIV (The Micro-Arcsecond Scintillation-Induced Variability) survey of 443 flat spectrum sources revealed that the IDV is related to the radio flux density and redshift. A study of the physical properties of these sources has been severely handicapped by the absence of reliable redshift measurements for many of these objects. This paper presents 79 new redshifts and a critical evaluation of 233 redshifts obtained from the literature. We classify spectroscopic identifications based on emission line properties, finding thatmore » 78% of the sources have broad emission lines and are mainly FSRQs. About 16% are weak lined objects, chiefly BL Lacs, and the remaining 6% are narrow line objects. The gross properties (redshift, spectroscopic class) of the MASIV sample are similar to those of other blazar surveys. However, the extreme compactness implied by ISS favors FSRQs and BL Lacs in the MASIV sample as these are the most compact object classes. We confirm that the level of IDV depends on the 5 GHz flux density for all optical spectral types. We find that BL Lac objects tend to be more variable than broad line quasars. The level of ISS decreases substantially above a redshift of about two. The decrease is found to be generally consistent with ISS expected for beamed emission from a jet that is limited to a fixed maximum brightness temperature in the source rest frame.« less

OPTICAL SPECTROSCOPIC OBSERVATIONS OF GAMMA-RAY BLAZAR CANDIDATES. V. TNG, KPNO, AND OAN OBSERVATIONS OF BLAZAR CANDIDATES OF UNCERTAIN TYPE IN THE NORTHERN HEMISPHERE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Álvarez Crespo, N.; Massaro, F.; Masetti, N.

The extragalactic γ-ray sky is dominated by emission from blazars, a peculiar class of active galactic nuclei. Many of the γ-ray sources included in the Fermi-Large Area Telescope Third Source catalog (3FGL) are classified as blazar candidates of uncertain type (BCUs) because there are no optical spectra available in the literature to confirm their nature. In 2013, we started a spectroscopic campaign to look for the optical counterparts of the BCUs and of the unidentified γ-ray sources to confirm their blazar nature. Whenever possible we also determine their redshifts. Here, we present the results of the observations carried out inmore » the northern hemisphere in 2013 and 2014 at the Telescopio Nazionale Galileo, Kitt Peak National Observatory, and Observatorio Astronómico Nacional in San Pedro Mártir. In this paper, we describe the optical spectra of 25 sources. We confirmed that all of the 15 BCUs observed in our campaign and included in our sample are blazars and we estimated the redshifts for three of them. In addition, we present the spectra for three sources classified as BL Lacs in the literature but with no optical spectra available to date. We found that one of them is a quasar (QSO) at a redshift of z = 0.208 and the other two are BL Lacs. Moreover, we also present seven new spectra for known blazars listed in the Roma-BZCAT that have an uncertain redshift or are classified as BL Lac candidates. We found that one of them, 5BZB J0724+2621, is a “changing look” blazar. According to the spectrum available in the literature, it was classified as a BL Lac, but in our observation we clearly detected a broad emission line that led us to classify this source as a QSO at z = 1.17.« less

BL Lacertae: X-ray spectral evolution and a black-hole mass estimate

NASA Astrophysics Data System (ADS)

Titarchuk, Lev; Seifina, Elena

2017-06-01

We present an analysis of the spectral properties observed in X-rays from active galactic nucleus BL Lacertae using RXTE, Suzaku, ASCA, BeppoSAX, and Swift observations. The total time covered by these observations is approximately 20 yr. We show strong observational evidence that this source undergoes X-ray spectral transitions from the low hard state (LHS) through the intermediate state (IS) to the high soft state (HSS) during these observations. During the RXTE observations (1997-2001, 180 ks, for a total 145 datasets), the source was approximately 75%, 20% and only 5% of the time in the IS, LHS, and HSS, respectively. We also used Swift observations (470 datasets, for a total 800 ks), which occurred during 12 yr (2005-2016), the broadband (0.3-200 keV) data of BeppoSAX (1997-2000, 160 ks), and the low X-ray energy (0.3-10 keV) data of ASCA (1995-1999, 160 ks). Two observations of Suzaku (2006, 2013; 50 ks) in combinations with long-term RXTE and Swift data-sets fortunately allow us to describe all spectral states of BL Lac. The spectra of BL Lac are well fitted by the so-called bulk motion Comptonization (BMC) model for all spectral states. We have established the photon index saturation level, Γsat = 2.2 ± 0.1, in the Γ versus mass accretion rate (Ṁ) correlation. This Γ - Ṁ correlation allows us to estimate the black-hole (BH) mass in BL Lac to be MBH 3 × 107M⊙ for a distance of 300 Mpc. For the BH mass estimate, we use the scaling method taking stellar-mass Galactic BHs 4U 1543-47 and GX 339-4 as reference sources. The Γ - Ṁ correlation revealed in BL Lac is similar to those in a number of stellar-mass Galactic BHs and two recently studied intermediate-mass extragalactic BHs. It clearly shows the correlation along with the very extended Γ saturation at 2.2. This is robust observational evidence for the presence of a BH in BL Lac. We also reveal that the seed (disk) photon temperatures are relatively low, of order of 100 eV, which are consistent with a high BH mass in BL Lac. It is worthwhile to emphasize that we found particular events when X-ray emission anti-correlates with radio emission. This effect indicates that mass accretion rate (and thus X-ray radiation) is higher when the mass outflow is lower.

Soft X-ray spectrum of BL Lacertae object AO 0235+164 as a tracer of elemental abundances at z approximately 0.5

NASA Technical Reports Server (NTRS)

Madejski, Greg

1994-01-01

We report the soft X-ray spectrum of BL Lac object AO 0235+164, observed with the Einstein Observatory Imaging Proportional Counter (IPC). This object (z = 0.94) has an intervening galaxy (or a protogalactic disk) at z = 0.524 present in the line of sight, producing both radio and optical absorption lines in the background BL Lac continuum. The X-ray spectrum exhibits a substantial soft X-ray cutoff, corresponding to several times that expected from our own Galaxy; we interpret that excess cutoff as due to the intervening galaxy. The comparison of the hydrogen column density inferred from the 21 cm radio data and the X-ray absorption allows, in principle, the determination of the elemental abundances in the intervening galaxy. However, the uncertainties in both the H I spin temperature and X-ray spectral parameters only loosely restrict these abundances to be 2 +/- 1 solar, which even at the lower limit appears higher than that inferred from studies of samples of optical absoprtion-line systems.

The flaring activity of Markarian 421 during April 2000

NASA Astrophysics Data System (ADS)

Fegan, D. J.; VERITAS Collaboration

2001-08-01

Evidence for correlated TeV γ and X-ray flaring of the extreme blazar Mrk421 during April 2000 is presented and discussed. The remarkably persistent TeV flare of April 30th 2000 (40 σ significance), exhibiting structure over almost six hours of continuous observation, is analysed in detail. 1 Extreme BL Lac objects The most extreme members of the Active Galactic Nucleus (AGN) family are BL Lac objects and optically violently variable (OVV) quasars, collectively known as blazars. These objects are dominated by the presence of relativistic jets. For jets fortuitously aligned with an observers line of sight, emission may exhibit dramatic variability over very short time scales, in turn implying remarkably compact emission regions. For blazars, the Spectral Energy Distribution (SED) is dominated by non-thermal continuum emission, extending from radio to TeV gamma rays. The broadband nature of the blazar emission offers unique insights into energetic physical processes at work in a very compact region, close to the base of the jet and near the underlying central engine, most likely a supermassive black hole. BL Lacs are very effectively characterized on the basis of their SED shape. X-ray and radio flux limited surveys apear to display a bimodal distribution of properties, with LBL (Low-energy peaked, or "Red" BL Lacs) having synchrotron peaks in the IR-optical bands, and HBL (High-energy peaked, or "Blue" BL Lacs) in the UV to soft X-ray band. Recent comprehensive surveys such as DXRBS, REX and RGB have extended, by almost two orders of magnitude, the range of observable synchrotron peak frequencies. For blazar class objects, broadband emission confirms that the synchrotron peak may span the entire IR Xray range, thus accounting for the multi-frequency emission properties of this class of object. Mrk421, Mrk501, 1ES2344 and 1H1426 all exhibit broadband emission properties, high

Temporal Variation of HCO+ 1_0 Galactic Absorption Lines Toward NRAO 150 and BL Lac

NASA Astrophysics Data System (ADS)

Han, Junghwan; Yun, Youngjoo; Park, Yong-Sun

2017-12-01

We present observations of HCO^+ 1-0 absorption lines toward two extragalactic compact radio sources, NRAO 150 and BL Lac with the Korean VLBI Network in order to investigate their time variation over 20 years by Galactic foreground clouds. It is found that the line shape of -17 km s^{-1} component changed marginally during 1993-1998 period and has remained unaltered thereafter for NRAO 150. Its behavior is different from that of H_2CO 1_{10}-1_{11}, suggesting chemical differentiation on ˜ 20 AU scale, the smallest ever seen. On the other hand, BL Lac exhibits little temporal variation for the HCO^+ and H_2CO lines. Our observation also suggests that Korea VLBI Network performs reliably in the spectrum mode in that the shapes of the new HCO^+ 1-0 spectra are in good agreement with the previous ones to an accuracy of a few percent except the time varying component toward NRAO 150.

GLAST answers about high-energy peaked BL Lacs: double-humped {gamma}-ray peak and extreme accelerators?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Costamante, L.; Aharonian, F.; Khangulyan, D.

2007-07-12

An often overlooked fact is that the MeV-GeV emission from High-energy peaked BL Lacs (HBL) is basically unknown: there are only 3 objects of this type among all EGRET identified blazars with measured spectra. GLAST will be able to measure the spectrum for many of them, in particular TeV-blazars, and surprises are expected. GLAST will tell if the {gamma}-ray peak in some HBL is actually a ''double peak'', as suggested by the comparison of EGRET and HESS data in PKS 2155-304, We also remind and argue that a new class of BL Lacs could exist, where particles are shock-accelerated nearmore » the maximum possible rate, characterized by the synchrotron emission peaking in the GLAST band (100 MeV - few GeV). Such objects could easily have escaped detection or identification so far, and could now be unveiled by GLAST.« less

3FGLzoo: classifying 3FGL unassociated Fermi-LAT γ-ray sources by artificial neural networks

NASA Astrophysics Data System (ADS)

Salvetti, D.; Chiaro, G.; La Mura, G.; Thompson, D. J.

2017-09-01

In its first four years of operation, the Fermi-Large Area Telescope (LAT) detected 3033 γ-ray emitting sources. In the Fermi-LAT Third Source Catalogue (3FGL) about 50 per cent of the sources have no clear association with a likely γ-ray emitter. We use an artificial neural network algorithm aimed at distinguishing BL Lacs from FSRQs to investigate the source subclass of 559 3FGL unassociated sources characterized by γ-ray properties very similar to those of active galactic nuclei. Based on our method, we can classify 271 objects as BL Lac candidates, 185 as FSRQ candidates, leaving only 103 without a clear classification. We suggest a new zoo for γ-ray objects, where the percentage of sources of uncertain type drops from 52 per cent to less than 10 per cent. The result of this study opens up new considerations on the population of the γ-ray sky, and it will facilitate the planning of significant samples for rigorous analyses and multiwavelength observational campaigns.

Kepler Observations of Rapid Optical Variability in the BL Lac Object W2r192+42

NASA Technical Reports Server (NTRS)

R.Edelson; Mushotzky, R.; Vaughn, S.; Scargle, J.; Gandhi, P.; Malkan, M.; Baumgartner, W.

2013-01-01

We present the first Kepler monitoring of a strongly variable BL Lac, W2R1926+42. The light curve covers 181 days with approx. 0.2% errors, 30 minute sampling and >90% duty cycle, showing numerous delta-I/I > 25% flares over timescales as short as a day. The flux distribution is highly skewed and non-Gaussian. The variability shows a strong rms-flux correlation with the clearest evidence to date for non-linearity in this relation. We introduce a method to measure periodograms from the discrete autocorrelation function, an approach that may be well-suited to a wide range of Kepler data. The periodogram is not consistent with a simple power-law, but shows a flattening at frequencies below 7x10(exp -5) Hz. Simple models of the power spectrum, such as a broken power law, do not produce acceptable fits, indicating that the Kepler blazar light curve requires more sophisticated mathematical and physical descriptions than currently in use.

X-ray and TeV Gamma-Ray Emission from Parallel Electron-Positron or Electron-Proton Beams in BL Lac Objects

NASA Astrophysics Data System (ADS)

Krawczynski, Henric

2007-04-01

In this contribution we discuss models of the X-rays and TeV gamma-ray emission from BL Lac objects based on parallel electron-positron or electron-proton beams that form close to the central black hole owing to the strong electric fields generated by the accretion disk and possibly also by the black hole itself. Fitting the energy spectrum of the BL Lac object Mrk 501, we obtain tight constrains on the beam properties. Launching a sufficiently energetic beam requires rather strong magnetic fields close to the black hole 100-1000 G. However, the model fits imply that the magnetic field in the emission region is only 0.02 G. Thus, the particles are accelerated close to the black hole and propagate a considerable distance before instabilities trigger the dissipation of energy through synchrotron and self-Compton emission. We discuss various approaches to generate enough power to drive the jet and, at the same time, to accelerate particles to 20 TeV energies. Although the parallel beam model has its own problems, it explains some of the long-standing problems that plague models based on Fermi type particle acceleration, like the presence of a very high minimum Lorentz factor of accelerated particles. We conclude with a brief discussion of the implications of the model for the difference between the processes of jet formation in BL Lac type objects and in quasars.

[Analgesia evoked by combined effect of corvitin and low-intensity microwaves on acupuncture points in mice of different genetic strains with somatic pain].

PubMed

Hura, O V; Bahats'ka, O V; Lymans'kyĭ, Iu P

2011-01-01

The level of analgesia has been investigated in mice of two genotypes C57BL/6J Bl/6j and CBA/CaLac with the somatic pain caused by the formalin test after irradiation of acupuncture point E-36 by microwaves of low intensity (30-300 GHz, density of a stream of capacity of 3-10-9 B(T)/cm2) on a background entered corvitin (20 mg/kg). It is shown, that the action of these two factors causes significant analgesia with different levels: 43% in C57BL/6J Bl/6j mice and 33% in CBA/CaLac mice. The intensity of analgesia after action of microwaves and corvitin exceeds the level attained during separate use of these factors.

Fermi LAT detection of a GeV flare from the high redshift BL Lac object PKS 2131-021

NASA Astrophysics Data System (ADS)

Ciprini, Stefano

2012-08-01

The Large Area Telescope (LAT), one of the two instruments on the Fermi Gamma-ray Space Telescope, has observed gamma-ray flaring activity from a source positionally consistent with the BL Lac object PKS 2131-021 (also known as 2FGL J2133.8-0154, Nolan et al. 2012, ApJS, 199, 31, and 4C -02.81) placed at radio coordinates R.A.: 323.5429567 deg, Dec.: -1.8881217 deg (J2000, Johnston et al. 1995, AJ, 110, 880).

Fermi LAT detection of a GeV flare from the BL Lac object Mrk 421

NASA Astrophysics Data System (ADS)

D'Ammando, F.; Orienti, M.

2012-07-01

The Large Area Telescope (LAT), one of the two instruments on the Fermi Gamma-ray Space Telescope, has observed gamma-ray flaring activity from a source positionally consistent with the BL Lac object Mrk 421 (also known as 2FGL J1104.4+3812, Nolan et al. 2012, ApJS, 199, 31; R.A.= 11h04m27.3139s, Dec.= +38d12m31.799s, J2000.0, Fey et al. 2004, AJ, 127, 3587) at redshift z=0.03 (De Vaucouleurs et al.

Testing the Pairs-Reflection Model with X-Ray Spectral Variability and X-Ray Properties of Complete Samples of Radio-Selected BL Lacertae Objects

NASA Astrophysics Data System (ADS)

Urry, C. Megan

1997-01-01

This grant was awarded to Dr. C. Megan Urry of the Space Telescope Science Institute in response to two successful ADP proposals to use archival Ginga and Rosat X-ray data for 'Testing the Pairs-Reflection model with X-Ray Spectral Variability' (in collaboration with Paola Grandi, now at the University of Rome) and 'X-Ray Properties of Complete Samples of Radio-Selected BL Lacertae Objects' (in collaboration with then-graduate student Rita Sambruna, now a post-doc at Goddard Space Flight Center). In addition, post-docs Joseph Pesce and Elena Pian, and graduate student Matthew O'Dowd, have worked on several aspects of these projects. The grant was originally awarded on 3/01/94; this report covers the full period, through May 1997. We have completed our project on the X-ray properties of radio-selected BL Lacs.

Radio morphology and parent population of X-ray selected BL Lacertae objects

NASA Technical Reports Server (NTRS)

Laurent-Muehleisen, S. A.; Kollgaard, R. I.; Moellenbrock, G. A.; Feigelson, E. D.

1993-01-01

High-dynamic range (typically 1700:1) radio maps of 15 X-ray BL Lac (XBL) objects from the HEAO-1 Large Area Sky Survey are presented. Morphological characteristics of these sources are compared with Fanaroff-Riley (FR) class I radio galaxies in the context of unified schemes, with reference to one-sided kiloparsec-scale emission. Evidence that cluster membership of XBLs is significantly higher than previously thought is also presented. It is shown that the extended radio powers, X-ray emission, core-to-lobe ratios, and linear sizes of the radio selected BL Lac (RBL) and XBL populations are consistent with an FR I radio galaxy parent population. A source list and VLA observing log and map parameters are provided.

Research and characterisation of blazar candidates among the Fermi/LAT 3FGL catalogue using multivariate classifications

NASA Astrophysics Data System (ADS)

Lefaucheur, Julien; Pita, Santiago

2017-06-01

Context. In the recently published 3FGL catalogue, the Fermi/LAT collaboration reports the detection of γ-ray emission from 3034 sources obtained after four years of observations. The nature of 1010 of those sources is unknown, whereas 2023 have well-identified counterparts in other wavelengths. Most of the associated sources are labelled as blazars (1717/2023), but the BL Lac or FSRQ nature of 573 of these blazars is still undetermined. Aims: The aim of this study was two-fold. First, to significantly increase the number of blazar candidates from a search among the large number of Fermi/LAT 3FGL unassociated sources (case A). Second, to determine the BL Lac or FSRQ nature of the blazar candidates, including those determined as such in this work and the blazar candidates of uncertain type (BCU) that are already present in the 3FGL catalogue (case B). Methods: For this purpose, multivariate classifiers - boosted decision trees and multilayer perceptron neural networks - were trained using samples of labelled sources with no caution flag from the 3FGL catalogue and carefully chosen discriminant parameters. The decisions of the classifiers were combined in order to obtain a high level of source identification along with well controlled numbers of expected false associations. Specifically for case A, dedicated classifications were generated for high (| b | >10◦) and low (| b | ≤10◦) galactic latitude sources; in addition, the application of classifiers to samples of sources with caution flag was considered separately, and specific performance metrics were estimated. Results: We obtained a sample of 595 blazar candidates (high and low galactic latitude) among the unassociated sources of the 3FGL catalogue. We also obtained a sample of 509 BL Lacs and 295 FSRQs from the blazar candidates cited above and the BCUs of the 3FGL catalogue. The number of expected false associations is given for different samples of candidates. It is, in particular, notably low ( 9/425) for the sample of high-latitude blazar candidates from case A. Full Tables 5 and 7 are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/602/A86

Magnetic Fields in Blazar Jets: Jet-Alignment of Radio and Optical Polarization over 20-30 Years

NASA Astrophysics Data System (ADS)

Wills, Beverley J.; Aller, M. F.; Caldwell, C.; Aller, H. D.

2012-01-01

Blazars are highly active nuclei of distant galaxies. They produce synchrotron-emitting relativistic jets on scales of less than a parsec to many Kpc. When viewed head-on, as opposed to in the plane of the sky, the jet motion appears superluminal, and the emission is Doppler boosted. Blazars show rapid radio and optical variability in flux density and polarization. There are two types of blazars that can have strong synchrotron continua: some quasars with strong broad emission lines, and BL Lac objects with weak or undetected broad lines. We have compiled optical linear polarization measurements of more than 100 blazars, including archival data from McDonald Observatory. While the optical data are somewhat sparsely sampled, The University of Michigan Radio Astronomical Observatory observed many blazars over 20-30 years, often well-sampled over days to weeks, enabling quasi-simultaneous comparison of optical and radio polarization position angles (EVPAs). We also collected data on jet direction -- position angles of the jet component nearest the radio core. The project is unique in examining the polarization and jet behavior over many years. BL Lac objects tend to have stable optically thin EVPA in the jet direction, meaning magnetic field is perpendicular to jet flow, often interpreted as the magnetic field compressed by shocks. In quasar-blazars optical and radio EVPA often changes between parallel or perpendicular to the jet direction, even in the same object. The underlying B field of the jet is is parallel to the flow, with approximately 90 degree changes resulting from shocks. For both BL Lac objects & quasars, the scatter in EVPA usually increases from low frequencies (4.8 GHz) through 14.5 GHz through optical. The wide optical-radio frequency range allows us to investigate optical depth effects and the spatial origin of radio and optical emission.

Multi-epoch intranight optical monitoring of eight radio-quiet BL Lac candidates

NASA Astrophysics Data System (ADS)

Kumar, P.; Gopal-Krishna; Stalin, C. S.; Chand, H.; Srianand, R.; Petitjean, P.

2017-10-01

For a new sample of eight weak-line quasars (WLQs) we report a sensitive search in 20 intranight monitoring sessions, for blazar-like optical flux variations on hour-like and longer time-scale (day/month/year-like). The sample consists exclusively of the WLQs that are not radio-loud and either have been classified as 'radio-weak probable BL Lac candidates' and/or are known to have exhibited at least one episode of large, blazar-like optical variability. Whereas only a hint of intranight variability is seen for two of these WLQs, J104833.5+620305.0 (z = 0.219) and J133219.6+622715.9 (z = 3.15), statistically significant internight variability at a few per cent level is detected for three of the sources, including the radio-intermediate WLQ J133219.6+622715.9 (z = 3.15) and the well-known bona fide radio-quiet WLQs J121221.5+534128.0 (z = 3.10) and WLQ J153259.9-003944.1 (z = 4.62). In the rest frame, this variability is intraday and in the far-ultraviolet band. On the time-scale of a decade, we find for three of the WLQs large brightness changes, amounting to 1.655 ± 0.009, 0.163 ± 0.010 and 0.144 ± 0.018 mag, for J104833.5+620305.0, J123743.1+630144.9 and J232428.4+144324.4, respectively. Whereas the latter two are confirmed radio-quiet WLQs, the extragalactic nature of J104833.5+620305.0 remains to be well established, thanks to the absence of any feature(s) in its available optical spectra. This study forms a part of our ongoing campaign of intranight optical monitoring of radio-quiet WLQs, in order to improve the understanding of this enigmatic class of active galactic nuclei and to look among them for a possible tiny, elusive population of radio-quiet BL Lacs.

Host galaxy and environment of the BL Lacertae object PKS 0548-322: Observations with subarcsecond resolution

NASA Technical Reports Server (NTRS)

Falomo, Renato; Pesce, Joseph E.; Treves, Aldo

1995-01-01

We report on direct, subarcsecond resolution imaging of the nebulosity and spectroscopy of galaxies in the field of the BL Lacertae object PKS 0548-322. Surface photometry of the nebulosity is used to derive the properties of the host galaxy (M(sub V) = -23.4), which exhibits signs of interaction with a close companion galaxy at approximately 25 kpc. The radial brightness profile of the nebulosity is well fitted by the contribution of a bulge (r(exp 1/4)) plus a point source and a small internal disk. An analysis of the galaxies in the field shows that the source is located in a rich cluster of galaxies. Spectra of five galaxies in the field indicate that they are at the same redshift as the BL Lac object, thus supporting the imaging result of a surrounding cluster associated with the BL Lac. This cluster is most likely Abell S0549.

Search for Cross-Correlations of Ultrahigh-Energy Cosmic Rays with BL Lacertae Objects

NASA Astrophysics Data System (ADS)

Abbasi, R. U.; Abu-Zayyad, T.; Amann, J. F.; Archbold, G.; Belov, K.; Belz, J. W.; BenZvi, S.; Bergman, D. R.; Blake, S. A.; Boyer, J. H.; Burt, G. W.; Cao, Z.; Connolly, B. M.; Deng, W.; Fedorova, Y.; Findlay, J.; Finley, C. B.; Hanlon, W. F.; Hoffman, C. M.; Holzscheiter, M. H.; Hughes, G. A.; Hüntemeyer, P.; Jui, C. C. H.; Kim, K.; Kirn, M. A.; Knapp, B. C.; Loh, E. C.; Maestas, M. M.; Manago, N.; Mannel, E. J.; Marek, L. J.; Martens, K.; Matthews, J. A. J.; Matthews, J. N.; O'Neill, A.; Painter, C. A.; Perera, L.; Reil, K.; Riehle, R.; Roberts, M. D.; Rodriguez, D.; Sasaki, M.; Schnetzer, S. R.; Seman, M.; Sinnis, G.; Smith, J. D.; Snow, R.; Sokolsky, P.; Springer, R. W.; Stokes, B. T.; Thomas, J. R.; Thomas, S. B.; Thomson, G. B.; Tupa, D.; Westerhoff, S.; Wiencke, L. R.; Zech, A.; HIRES Collaboration

2006-01-01

Data taken in stereo mode by the High Resolution Fly's Eye (HiRes) air fluorescence experiment are analyzed to search for correlations between the arrival directions of ultrahigh-energy cosmic rays with the positions of BL Lacertae objects. Several previous claims of significant correlations between BL Lac objects and cosmic rays observed by other experiments are tested. These claims are not supported by the HiRes data. However, we verify a recent analysis of correlations between HiRes events and a subset of confirmed BL Lac objects from the 10th Veron Catalog, and we study this correlation in detail. Due to the a posteriori nature of the search, the significance level cannot be reliably estimated and the correlation must be tested independently before any claim can be made. We identify the precise hypotheses that will be tested with statistically independent data.

Mouse Leydig Cells with Different Androgen Production Potential Are Resistant to Estrogenic Stimuli but Responsive to Bisphenol A Which Attenuates Testosterone Metabolism

PubMed Central

Savchuk, Iuliia; Söder, Olle; Svechnikov, Konstantin

2013-01-01

It is well known that estrogens and estrogen-like endocrine disruptors can suppress steroidogenic gene expression, attenuate androgen production and decrease differentiation of adult Leydig cell lineage. However, there is no information about the possible link between the potency of Leydig cells to produce androgens and their sensitivity to estrogenic stimuli. Thus, the present study explored the relationship between androgen production potential of Leydig cells and their responsiveness to estrogenic compounds. To investigate this relationship we selected mouse genotypes contrasting in sex hormone levels and differing in testosterone/estradiol (T/E2) ratio. We found that two mouse genotypes, CBA/Lac and C57BL/6j have the highest and the lowest serum T/E2 ratio associated with increased serum LH level in C57BL/6j compared to CBA/Lac. Analysis of steroidogenic gene expression demonstrated significant upregulation of Cyp19 gene expression but coordinated suppression of LHR, StAR, 3βHSDI and Cyp17a1 in Leydig cells from C57BL/6j that was associated with attenuated androgen production in basal and hCG-stimulated conditions compared to CBA/Lac mice. These genotype-dependent differences in steroidogenesis were not linked to changes in the expression of estrogen receptors ERα and Gpr30, while ERβ expression was attenuated in Leydig cells from C57BL/6j compared to CBA/Lac. No effects of estrogenic agonists on steroidogenesis in Leydig cells from both genotypes were found. In contrast, xenoestrogen bisphenol A significantly potentiated hCG-activated androgen production by Leydig cells from C57BL/6j and CBA/Lac mice by suppressing conversion of testosterone into corresponding metabolite 5α-androstane-3α,17β-diol. All together our data indicate that developing mouse Leydig cells with different androgen production potential are resistant to estrogenic stimuli, while xenoestrogen BPA facilitates hCG-induced steroidogenesis in mouse Leydig cells via attenuation of testosterone metabolism. This cellular event can cause premature maturation of Leydig cells that may create abnormal intratesticular paracrine milieu and disturb proper development of germ cells. PMID:23967237

Constraining the red shifts of TeV BL Lac objects

NASA Astrophysics Data System (ADS)

Qin, Longhua; Wang, Jiancheng; Yan, Dahai; Yang, Chuyuan; Yuan, Zunli; Zhou, Ming

2018-01-01

We present a model-dependent method to estimate the red shifts of three TeV BL Lac objects (BL Lacs) through fitting their (quasi-)simultaneous multi-waveband spectral energy distributions (SEDs) with a one-zone leptonic synchrotron self-Compton model. Considering the impact of electron energy distributions (EEDs) on the results, we use three types of EEDs to fit the SEDs: a power-law EED with exponential cut-off (PLC), a log-parabola (PLLP) EED and the broken power-law (BPL) EED. We also use a parameter α to describe the uncertainties of the extragalactic background light models, as in Abdo et al. We then use a Markov chain Monte Carlo method to explore the multi-dimensional parameter space and obtain the uncertainties of the model parameters based on the observational data. We apply our method to obtain the red shifts of three TeV BL Lac objects in the marginalized 68 per cent confidence, and find that the PLC EED does not fit the SEDs. For 3C66A, the red shift is 0.14-0.31 and 0.16-0.32 in the BPL and PLLP EEDs. For PKS1424+240, the red shift is 0.55-0.68 and 0.55-0.67 in the BPL and PLLP EEDs. For PG1553+113, the red shift is 0.22-0.48 and 0.22-0.39 in the BPL and PLLP EEDs. We also estimate the red shift of PKS1424+240 in the high stage to be 0.46-0.67 in the PLLP EED, roughly consistent with that in the low stage.

Quasi-simultaneous observations of the BL Lac object MK 501 in X-ray, UV, visible, IR and radio frequencies

NASA Technical Reports Server (NTRS)

Kondo, D. M.; Worrall, D. M.; Mushotzky, R. F.; Hackney, R. L.; Hackney, K. H.; Oke, J. B.; Yee, H.; Neugebauer, G.; Matthews, K.; Feldman, P. A.

1980-01-01

Quasi-simultaneous observations of the BL Lacertae (Lac) objects MK 501 were performed for the first time at X-ray, ultraviolet, visible, infrared, and radio frequencies. The observed spectral slope from the X-ray to UV regions is positive and continuous, but that from the mid UV to visible light region becomes gradually flat and possibly turns down toward lower frequencies; the optical radio emission can not be accounted for by a single power law. Several theoretical models were considered for the emission mechanism. A quantitative comparison was performed with the synchrotron-self-Compton model; the total spectrum is found consistent with this model. The spectrum from visible light to X-ray is consistent with synchrotron radiation or with inverse-Compton scattering by a hot thermal cloud of electrons. The continuity of the spectral slope from X-ray to UV implied by the current data suggests that the previous estimates of the total luminosity of this BL Lac object is underestimated by a factor of about three or four.

An Analysis of Periodic Components in BL Lac Object S5 0716 +714 with MUSIC Method

NASA Astrophysics Data System (ADS)

Tang, J.

2012-01-01

Multiple signal classification (MUSIC) algorithms are introduced to the estimation of the period of variation of BL Lac objects.The principle of MUSIC spectral analysis method and theoretical analysis of the resolution of frequency spectrum using analog signals are included. From a lot of literatures, we have collected a lot of effective observation data of BL Lac object S5 0716 + 714 in V, R, I bands from 1994 to 2008. The light variation periods of S5 0716 +714 are obtained by means of the MUSIC spectral analysis method and periodogram spectral analysis method. There exist two major periods: (3.33±0.08) years and (1.24±0.01) years for all bands. The estimation of the period of variation of the algorithm based on the MUSIC spectral analysis method is compared with that of the algorithm based on the periodogram spectral analysis method. It is a super-resolution algorithm with small data length, and could be used to detect the period of variation of weak signals.

The Spectral Energy Distribution of Fermi bright blazars

DOE PAGES

Abdo, A. A.; Ackermann, M.; Agudo, I.; ...

2010-05-13

We have conducted a detailed investigation of the broadband spectral properties of the γ-ray selected blazars of the Fermi LAT Bright AGN Sample (LBAS). By combining our accurately estimated Fermi γ-ray spectra with Swift, radio, infra-red, optical, and other hard X-ray/γ-ray data, collected within 3 months of the LBAS data taking period, we were able to assemble high-quality and quasi-simultaneous spectral energy distributions (SED) for 48 LBAS blazars. The SED of these γ-ray sources is similar to that of blazars discovered at other wavelengths, clearly showing, in the usual log ν-log ν F ν representation, the typical broadband spectral signaturesmore » normally attributed to a combination of low-energy synchrotron radiation followed by inverse Compton emission of one or more components. Here, we have used these SED to characterize the peak intensity of both the low- and the high-energy components. The results have been used to derive empirical relationships that estimate the position of the two peaks from the broadband colors (i.e., the radio to optical, α ro, and optical to X-ray, α ox, spectral slopes) and from the γ-ray spectral index. Our data show that the synchrotron peak frequency (ν S peak) is positioned between 10 12.5 and 10 14.5 Hz in broad-lined flat spectrum radio quasars (FSRQs) and between 10 13 and 10 17 Hz in featureless BL Lacertae objects. We find that the γ-ray spectral slope is strongly correlated with the synchrotron peak energy and with the X-ray spectral index, as expected at first order in synchrotron-inverse Compton scenarios. However, simple homogeneous, one-zone, synchrotron self-Compton (SSC) models cannot explain most of our SED, especially in the case of FSRQs and low energy peaked (LBL) BL Lacs. More complex models involving external Compton radiation or multiple SSC components are required to reproduce the overall SED and the observed spectral variability. While more than 50% of known radio bright high energy peaked (HBL) BL Lacs are detected in the LBAS sample, only less than 13% of known bright FSRQs and LBL BL Lacs are included. This suggests that the latter sources, as a class, may be much fainter γ-ray emitters than LBAS blazars, and could in fact radiate close to the expectations of simple SSC models. We categorized all our sources according to a new physical classification scheme based on the generally accepted paradigm for Active Galactic Nuclei and on the results of this SED study. Since the LAT detector is more sensitive to flat spectrum γ-ray sources, the correlation between ν S peak and γ-ray spectral index strongly favors the detection of high energy peaked blazars, thus explaining the Fermi overabundance of this type of sources compared to radio and EGRET samples. Finally, this selection effect is similar to that experienced in the soft X-ray band where HBL BL Lacs are the dominant type of blazars.« less

The Spectral Energy Distribution of Fermi Bright Blazars

NASA Technical Reports Server (NTRS)

Abdo, A. A.; Ackermann, M.; Agudo, I.; Ajello, M.; Aller, H. D.; Aller, M. F.; Angelakis, E.; Arkharov, A. A.; Axelsson, M.; Bach, U.;

MAGIC detection of very high energy γ-ray emission from the low-luminosity blazar 1ES 1741+196

DOE PAGES

Ahnen, M. L.; Ansoldi, S.; Antonelli, L. A.; ...

2017-02-23

Here, we present the first detection of the nearby (z = 0.084) low-luminosity BL Lac object 1ES 1741+196 in the very high energy (E > 100 GeV) band. This object lies in a triplet of interacting galaxies. Early predictions had suggested 1ES 1741+196 to be, along with several other high-frequency BL Lac sources, within the reach of MAGIC detectability. Its detection by MAGIC, later confirmed by VERITAS, helps to expand the small population of known TeV BL Lacs. The source was observed with the MAGIC telescopes between 2010 April and 2011 May, collecting 46 h of good quality data. Thesemore » observations led to the detection of the source at 6.0 σ confidence level, with a steady flux F(>100 GeV) = (6.4 ± 1.7stat ± 2.6syst) × 10–12 ph cm–2s–1 and a differential spectral photon index Γ = 2.4 ± 0.2stat ± 0.2syst in the range of ~80 GeV–3 TeV. To study the broad-band spectral energy distribution (SED) simultaneous with MAGIC observations, we use KVA, Swift/UVOT and XRT and Fermi/LAT data. One-zone synchrotron-self-Compton (SSC) modelling of the SED of 1ES 1741+196 suggests values for the SSC parameters that are quite common among known TeV BL Lacs except for a relatively low Doppler factor and slope of electron energy distribution. A thermal feature seen in the SED is well matched by a giant elliptical's template. As a result, this appears to be the signature of thermal emission from the host galaxy, which is clearly resolved in optical observations.« less

The X-ray spectra of the BL Lacertae objects PKS 0548 - 322 and 3C 66A

NASA Technical Reports Server (NTRS)

Maccacaro, T.; Maccagni, D.; Tarenghi, M.

1983-01-01

Einstein Observatory simultaneous imaging proportional counter and monitor proportional counter data are combined in order to derive the energy spectra of the BL Lac objects PKS 0548-322 and 3C 66A between 0.2 and 10 keV. While the latter is found to be variable in both intensity and spectral shape, the former, although constant in the present data, is found to have experienced a spectrum variation in view of results from other experiments. Attention is given to the implications of flux and spectral variability in BL Lac objects for models of X-ray emission mechanisms. It is suggested that the wide spread of the spectral index distribution is due to the detection of the highly variable synchrotron-produced X-rays that are generally undetected in QSOs.

Fermi-LAT detection of a GeV flare from the BL Lac object 1ES 2322-409

NASA Astrophysics Data System (ADS)

Ciprini, Stefano

2013-10-01

The Large Area Telescope (LAT), one of the two instruments on the Fermi Gamma-ray Space Telescope, has observed an increasing gamma-ray flux from a source positionally consistent with the BL Lac object 1ES 2322-409 (also know as 2FGL J2324.7-4042, Nolan et al. 2012, ApJS, 199, 31, and as 1FHL J2324.6-4041, Ackermann et al., ApJS, submitted, arXiv:1306.6772), with 2MASS counterpart coordinates, (J2000.0), R.A.: 351.18612 deg, Dec: -40.68036 deg (Mao 2011, New Ast., 16, 503).

The Luminosity Function of the Host Galaxies of QSOs and BL Lac Objects

NASA Astrophysics Data System (ADS)

Carangelo, Nicoletta; Falomo, Renato; Treves, Aldo

A clear insight of the galaxies hosting active galactic nuclei is of fundamental importance for understanding the processes of galaxies and nuclei formation and their cosmic evolution. A good characterization of the host galaxies properties requires images of excellent quality in order to disentangle the light of the galaxy from that of the bright nucleus. To this aim HST has provided a major improvement of data on QSOs (Disney et al. 1995; Bahcall et al. 1996,1997; Boyce et al. 1998; McLure et al. 1999; Hamilton et al. 2000; Kukula et al. 2001) and BL Lacs (Scarpa et al. 2000, Urry et al. 2000).

VizieR Online Data Catalog: Gamma-ray AGN type determination (Hassan+, 2013)

NASA Astrophysics Data System (ADS)

Hassan, T.; Mirabal, N.; Contreras, J. L.; Oya, I.

2013-11-01

In this paper, we employ Support Vector Machines (SVMs) and Random Forest (RF) that embody two of the most robust supervised learning algorithms available today. We are interested in building classifiers that can distinguish between two AGN classes: BL Lacs and FSRQs. In the 2FGL, there is a total set of 1074 identified/associated AGN objects with the following labels: 'bzb' (BL Lacs), 'bzq' (FSRQs), 'agn' (other non-blazar AGN) and 'agu' (active galaxies of uncertain type). From this global set, we group the identified/associated blazars ('bzb' and 'bzq' labels) as the training/testing set of our algorithms. (2 data files).

Resolving the host galaxy of a distant blazar with LBT/LUCI 1 + ARGOS

NASA Astrophysics Data System (ADS)

Farina, E. P.; Georgiev, I. Y.; Decarli, R.; Terzić, T.; Busoni, L.; Gässler, W.; Mazzoni, T.; Borelli, J.; Rosensteiner, M.; Ziegleder, J.; Bonaglia, M.; Rabien, S.; Buschkamp, P.; Orban de Xivry, G.; Rahmer, G.; Kulas, M.; Peter, D.

2018-05-01

BL Lac objects emitting in the very high energy (VHE) regime are unique tools to peer into the properties of the extragalactic background light (EBL). However, due to the typical absence of features in their spectra, the determination of their redshifts has proven challenging. In this work, we exploit the superb spatial resolution delivered by the new Advanced Rayleigh guided Ground layer adaptive Optics System (ARGOS) at the Large Binocular Telescope to detect the host galaxy of HESS J1943+213, a VHE emitting BL Lac shining through the Galaxy. Deep H-band imaging collected during the ARGOS commissioning allowed us to separate the contribution of the nuclear emission and to unveil the properties of the host galaxy with unprecedented detail. The host galaxy is well fitted by a Sérsic profile with index of n ˜ 2 and total magnitude of HHost ˜ 16.15 mag. Under the assumption that BL Lac host galaxies are standard candles, we infer a redshift of z ˜ 0.21. In the framework of the current model for the EBL, this value is in agreement with the observed dimming of the VHE spectrum due to the annihilation of energetic photons on the EBL

X-Ray and TeV Gamma-Ray Emission from Parallel Electron-Positron or Electron-Proton Beams in BL Lacertae Objects

NASA Astrophysics Data System (ADS)

Krawczynski, H.

2007-04-01

In this paper we discuss models of the X-ray and TeV γ-ray emission from BL Lac objects based on parallel electron-positron or electron-proton beams that form close to the central black hole, due to the strong electric fields generated by the accretion disk and possibly also by the black hole itself. Fitting the energy spectrum of the BL Lac object Mrk 501, we obtain tight constraints on the beam properties. Launching a sufficiently energetic beam requires rather strong magnetic fields close to the black hole (~100-1000 G). However, the model fits imply that the magnetic field in the emission region is only ~0.02 G. Thus, the particles are accelerated close to the black hole and propagate a considerable distance before instabilities trigger the dissipation of energy through synchrotron and self-Compton emission. We discuss various approaches to generate enough power to drive the jet and, at the same time, to accelerate particles to ~20 TeV energies. Although the parallel beam model has its own problems, it explains some of the long-standing problems that plague models based on Fermi-type particle acceleration, such as the presence of a very high minimum Lorentz factor of accelerated particles. We conclude with a brief discussion of the implications of the model for the difference between the processes of jet formation in BL Lac-type objects and those in quasars.

Very high energy gamma-ray emission detected from PKS 1440-389 with H.E.S.S.

NASA Astrophysics Data System (ADS)

Hofmann, W.

2012-04-01

The BL Lac object PKS 1440-389, located at a tentative redshift of z=0.065 (6dF Galaxy Survey, Jones, D.H. et al. MNRAS 355, 747-763, 2004), has been reported as a hard (G=1.75+/-0.05), bright, and steady extragalactic source at GeV energies in the Fermi-LAT catalogue (2FGL J1443.9-3908, P.L. Nolan et al., 2012, ApJS, 199, 31). The extrapolation of the Fermi-LAT spectrum to very high energies (VHE; E> 100 GeV), together with its brightness in the radio and X-ray bands, makes this BL Lac object a good candidate for VHE emission.

Discovery of Very High Energy Gamma-Ray Emission from BL Lac object H1722+119 by the MAGIC Telescopes

NASA Astrophysics Data System (ADS)

Cortina, Juan

2013-05-01

H1722+119 is a BL Lac object, that was listed as candidate TeV blazar in Costamante & Ghisellini (2002) based on its X-ray and radio properties. Its redshift is uncertain; Sbarufatti et al. 2006 give z>0.17. The source has been detected by Fermi-LAT, in the Second Fermi Catalog with F(>1 GeV) (3.7+-0.3)e-09 cm^-2 s^-1 and with spectral index 1.92+-0.06. H1722+119 was observed for five nights by the MAGIC telescopes starting May 17th 2013 and collecting 11 hours of good quality data.

A revised and updated catalog of quasi-stellar objects

NASA Technical Reports Server (NTRS)

Hewitt, A.; Burbidge, G.

1993-01-01

The paper contains a catalog of all known quasi-stellar objects (QSOs) with measured emission redshifts, and BL Lac objects, complete to 1992 December 31. The catalog contains 7315 objects, nearly all QSOs including about 90 BL Lac objects. The catalog and references contain extensive information on names, positions, magnitudes, colors, emission-line redshifts, absorption, variability, polarization, and X-ray, radio, and infrared data. A key in the form of subsidiary tables enables the reader to relate the name of a given object to its coordinate name, which is used throughout the compilation. Plots of the Hubble diagram, the apparent magnitude distribution, the emission redshift distribution, and the distribution of the QSOs on the sky are also given.

JET PROPERTIES OF GeV-SELECTED RADIO-LOUD NARROW-LINE SEYFERT 1 GALAXIES AND POSSIBLE CONNECTION TO THEIR DISK AND CORONA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, Xiao-Na; Lin, Da-Bin; Liang, En-Wei

The observed spectral energy distributions of five GeV-selected narrow-line Seyfert 1 (NLS1) galaxies are fitted with a model including the radiation ingredients from the relativistic jet, the accretion disk, and the corona. We compare the properties of these GeV NLS1 galaxies with flat spectrum radio quasars (FSRQs), BL Lacertae objects (BL Lacs), and radio-quiet (RQ) Seyfert galaxies, and explore possible hints for jet-disk/corona connection. Our results show that the radiation physics and the jet properties of the GeV NLS1 galaxies resemble that of FSRQs. The luminosity variations of PMN J0948+0022 and 1H 0323+342 at the GeV band is tightly correlatedmore » with the beaming factor (δ), similar to that observed in FSRQ 3C 279. The accretion disk luminosities and the jet powers of the GeV NLS1 galaxies cover both the ranges of FSRQs and BL Lacs. With the detection of bright corona emission in 1H 0323+342, we show that the ratio of the corona luminosity (L {sub corona}) to the accretion disk luminosity (L {sub d}) is marginally within the high end of this ratio distribution for an RQ Seyfert galaxy sample, and the variation of jet luminosity may connect with L {sub corona}. However, it is still unclear whether a system with a high L {sub corona}/L {sub d} ratio prefers to power a jet.« less

The first catalog of active galactic nuclei detected by the FERMI large area telescope

DOE PAGES

Abdo, A. A.; Ackermann, M.; Ajello, M.; ...

2010-04-29

Here, we present the first catalog of active galactic nuclei (AGNs) detected by the Large Area Telescope (LAT), corresponding to 11 months of data collected in scientific operation mode. The First LAT AGN Catalog (1LAC) includes 671 γ-ray sources located at high Galactic latitudes (|b|>10°) that are detected with a test statistic greater than 25 and associated statistically with AGNs. Some LAT sources are associated with multiple AGNs, and consequently, the catalog includes 709 AGNs, comprising 300 BL Lacertae objects, 296 flat-spectrum radio quasars, 41 AGNs of other types, and 72 AGNs of unknown type. We also classify the blazarsmore » based on their spectral energy distributions as archival radio, optical, and X-ray data permit. In addition to the formal 1LAC sample, we provide AGN associations for 51 low-latitude LAT sources and AGN "affiliations" (unquantified counterpart candidates) for 104 high-latitude LAT sources without AGN associations. The overlap of the 1LAC with existing γ-ray AGN catalogs (LBAS, EGRET, AGILE, Swift, INTEGRAL, TeVCat) is briefly discussed. Various properties—such as γ-ray fluxes and photon power-law spectral indices, redshifts, γ-ray luminosities, variability, and archival radio luminosities—and their correlations are presented and discussed for the different blazar classes. Lastly, we compare the 1LAC results with predictions regarding the γ-ray AGN populations, and we comment on the power of the sample to address the question of the blazar sequence.« less

Analysis of Microvariable Activity of BL Lacertae

NASA Astrophysics Data System (ADS)

Sadun, Alberto C.; Asadi-Zeydabadi, Masoud; Hindman, Lauren; Moody, J. Ward

2018-06-01

BL Lac is a low-frequency peaked blazar (LBL) which emits synchrotron radiation at near-IR and optical wavelengths. Therefore optical observations are helpful in, among other things, studying the acceleration and cooling timescales of the electrons in the relativistic jets. We have made very high cadence observations of BL Lac over a number of nights with the Remote Observatory for Variable Object Research (ROVOR). Each night shows secular drift as well as a number of microvariable events lasting only a few minutes each. These data were then processed, compiled, and analyzed in order to examine the underlying mechanism that resulted in such activity. A geometric model is introduced that has worked well in the past on other similar sources.Our relativistic jet model consists of a slowly varying beamed source that already appears bright because it lies nearly to our line of sight. From this, individual relativistic components are ejected a few degrees relative to the aforementioned beaming angle. This is what we believe is responsible for the microvariability emission.

Magnetic Black Hole Waves

NASA Image and Video Library

2015-07-09

This cartoon shows how magnetic waves, called Alfvén S-waves, propagate outward from the base of black hole jets. The jet is a flow of charged particles, called a plasma, which is launched by a black hole. The jet has a helical magnetic field (yellow coil) permeating the plasma. The waves then travel along the jet, in the direction of the plasma flow, but at a velocity determined by both the jet's magnetic properties and the plasma flow speed. The BL Lac jet examined in a new study is several light-years long, and the wave speed is about 98 percent the speed of light. Fast-moving magnetic waves emanating from a distant supermassive black hole undulate like a whip whose handle is being shaken by a giant hand, according to a study using data from the National Radio Astronomy Observatory's Very Long Baseline Array. Scientists used this instrument to explore the galaxy/black hole system known as BL Lacertae (BL Lac) in high resolution. http://photojournal.jpl.nasa.gov/catalog/PIA19822

Assessment of the Intestinal Barrier with Five Different Permeability Tests in Healthy C57BL/6J and BALB/cJ Mice.

PubMed

Volynets, Valentina; Reichold, Astrid; Bárdos, Gyöngyi; Rings, Andreas; Bleich, André; Bischoff, Stephan C

2016-03-01

Intestinal permeability is thought to be of major relevance for digestive and nutrition-related diseases, and therefore has been studied in numerous mouse models of disease. However, it is unclear which tools are the preferable ones, and how normal values should be defined. To compare different in vivo permeability tests in healthy mice of commonly used genetic backgrounds. We assessed the intestinal barrier in male and female C57BL/6J and BALB/cJ mice of different ages, using four orally administered permeability markers, FITC-dextran 4000 (FITC-D4000) and ovalbumin (OVA) measured in plasma, and polyethylene glycol (PEG) and lactulose/mannitol (Lac/Man) measured in urine, and by assessing lipopolysaccharide (LPS) in portal vein plasma. After gavage, FITC-D4000, OVA, Lac/Man, and PEG400, but not PEG4000, were detectable in plasma or urine. Female mice tended to have a higher permeability according to the FITC-D4000, OVA, and PEG400 tests, but the Lac/Man ratio was higher in males. No significant differences between the two mouse strains of young and old mice were observed except for mannitol recovery, which was higher in BALB/cJ mice compared to C57BL/6J mice (p < 0.05). Virtually no LPS was detected in healthy mice. For all markers, normal values have been defined based on 5th-95th percentile ranges of our data. Selected oral permeability tests, such as FITC-D4000, OVA, PEG400, and Lac/Man, as well as LPS measurements in portal vein plasma, could be suitable for the evaluation of the intestinal barrier in mice, if used in a standardized way.

Continuum radiation from active galactic nuclei: A statistical study

NASA Technical Reports Server (NTRS)

Isobe, T.; Feigelson, E. D.; Singh, K. P.; Kembhavi, A.

1986-01-01

The physics of the continuum spectrum of active galactic nuclei (AGNs) was examined using a large data set and rigorous statistical methods. A data base was constructed for 469 objects which include radio selected quasars, optically selected quasars, X-ray selected AGNs, BL Lac objects, and optically unidentified compact radio sources. Each object has measurements of its radio, optical, X-ray core continuum luminosity, though many of them are upper limits. Since many radio sources have extended components, the core component were carefully selected out from the total radio luminosity. With survival analysis statistical methods, which can treat upper limits correctly, these data can yield better statistical results than those previously obtained. A variety of statistical tests are performed, such as the comparison of the luminosity functions in different subsamples, and linear regressions of luminosities in different bands. Interpretation of the results leads to the following tentative conclusions: the main emission mechanism of optically selected quasars and X-ray selected AGNs is thermal, while that of BL Lac objects is synchrotron; radio selected quasars may have two different emission mechanisms in the X-ray band; BL Lac objects appear to be special cases of the radio selected quasars; some compact radio sources show the possibility of synchrotron self-Compton (SSC) in the optical band; and the spectral index between the optical and the X-ray bands depends on the optical luminosity.

POWERFUL HIGH-ENERGY EMISSION OF THE REMARKABLE BL Lac OBJECT S5 0716+714

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vittorini, V.; Chen, A. W.; Ferrari, A.

BL Lac objects of the intermediate subclass (IBLs) are known to emit a substantial fraction of their power in the energy range 0.1-10 GeV. Detecting gamma-ray emission from such sources provides therefore a direct probe of the emission mechanisms and of the underlying powerhouse. The gamma-ray satellite, AGILE, detected the remarkable IBL S5 0716+714 (z approx = 0.3) during a high state in the period from 2007 September-October, marked by two very intense flares reaching peak fluxes of 200 x 10{sup -8} photons cm{sup -2} s{sup -1} above 100 MeV, with simultaneous optical and X-ray observations. We present here amore » theoretical model for the two major flares and discuss the overall energetics of the source. We conclude that 0716+714 is among the brightest BL Lac's ever detected at gamma-ray energies. Because of its high power and lack of signs for ongoing accretion or surrounding gas, the source is an ideal candidate to test the maximal power extractable from a rotating supermassive black hole via the pure Blandford-Znajek (BZ) mechanism. We find that during the 2007 gamma-ray flares 0716+714 approached or just exceeded the upper limit set by BZ for a black hole of mass 10{sup 9} M{sub sun}.« less

The Spectral Energy Distributions of Blazars and the Connections among XBLs, RBLs, and OVV Quasars

NASA Astrophysics Data System (ADS)

Xie, Guangzhong; Dai, Benzhong; Liang, Enwei; Ma, Li; Jiang, Zejun

2001-06-01

In this paper, we introduce two composite spectral indices, αxox=αox-αx and αoro=αor-αo. In the composite color-color (αxoxαoro) diagram, there are three regions: (1) For XBLs and XBL-like RBLs, the values of αxox is in the range of -1.0 -0.0; (2) For RBLs, αxox is in 0.0-1.5, and αxox < -0.75; (3) For OVV quasars, αxox is in 0.0-1.5 and the αxox > -0.75. Our results support the conclusion that RBLs are intermediate between XBLs and OVV quasars, which has been given by Sambruna et al. (1996, AAA 65.159.120). On the other hand, based on the idea that a group of extragalactic objects which can be regarded as a category with similar physical properties must fit the same relation, using the HST new observational data, we found that there is a strong correlation between the apparent magnitude of the host galaxies and log z for all BL Lac objects (XBLs + RBLs), but OVV quasars lie about 4 magnitude above the Hubble line of 86 BL Lacs. This implies that all XBL-like and XBLs belong to a single category with similar physical properties, while OVV quasars and BL Lacs belong to different populations.

OPTICAL SPECTROSCOPY OF SDSS J004054.65-0915268: THREE POSSIBLE SCENARIOS FOR THE CLASSIFICATION. A z ∼ 5 BL LACERTAE, A BLUE FSRQ, OR A WEAK EMISSION LINE QUASAR

DOE Office of Scientific and Technical Information (OSTI.GOV)

Landoni, M.; Zanutta, A.; Bianco, A.

2016-02-15

The haunt of high-redshift BL Lacerate objects is day by day more compelling to firmly understand their intrinsic nature and evolution. SDSS J004054.65-0915268 is, at the moment, one of the most distant BL Lac candidates, at z ∼ 5. We present a new optical-near-IR spectrum obtained with ALFOSC-NOT with a new, custom designed dispersive grating aimed to detect broad emission lines that could disprove this classification. In the obtained spectra, we do not detect any emission features and we provide an upper limit to the luminosity of the C iv broad emission line. Therefore, the nature of the object is then discussed,more » building the overall spectral energy distribution (SED) and fitting it with three different models. Our fits, based on SED modeling with different possible scenarios, cannot rule out the possibility that this source is indeed a BL Lac object, though the absence of optical variability and the lack of strong radio flux seem to suggest that the observed optical emission originates from a thermalized accretion disk.« less

Optical Spectroscopy of SDSS J004054.65-0915268: Three Possible Scenarios for the Classification. A z ˜ 5 BL Lacertae, a Blue FSRQ, or a Weak Emission Line Quasar

NASA Astrophysics Data System (ADS)

Landoni, M.; Zanutta, A.; Bianco, A.; Tavecchio, F.; Bonnoli, G.; Ghisellini, G.

2016-02-01

The haunt of high-redshift BL Lacerate objects is day by day more compelling to firmly understand their intrinsic nature and evolution. SDSS J004054.65-0915268 is, at the moment, one of the most distant BL Lac candidates, at z ˜ 5. We present a new optical-near-IR spectrum obtained with ALFOSC-NOT with a new, custom designed dispersive grating aimed to detect broad emission lines that could disprove this classification. In the obtained spectra, we do not detect any emission features and we provide an upper limit to the luminosity of the C IV broad emission line. Therefore, the nature of the object is then discussed, building the overall spectral energy distribution (SED) and fitting it with three different models. Our fits, based on SED modeling with different possible scenarios, cannot rule out the possibility that this source is indeed a BL Lac object, though the absence of optical variability and the lack of strong radio flux seem to suggest that the observed optical emission originates from a thermalized accretion disk.

Validation of antibiotic residue tests for dairy goats.

PubMed

Zeng, S S; Hart, S; Escobar, E N; Tesfai, K

1998-03-01

The SNAP test, LacTek test (B-L and CEF), Charm Bacillus sterothermophilus var. calidolactis disk assay (BsDA), and Charm II Tablet Beta-lactam sequential test were validated using antibiotic-fortified and -incurred goat milk following the protocol for test kit validations of the U.S. Food and Drug Administration Center for Veterinary Medicine. SNAP, Charm BsDA, and Charm II Tablet Sequential tests were sensitive and reliable in detecting antibiotic residues in goat milk. All three assays showed greater than 90% sensitivity and specificity at tolerance and detection levels. However, caution should be taken in interpreting test results at detection levels. Because of the high sensitivity of these three tests, false-violative results could be obtained in goat milk containing antibiotic residues below the tolerance level. Goat milk testing positive by these tests must be confirmed using a more sophisticated methodology, such as high-performance liquid chromatography, before the milk is condemned. LacTek B-L test did not detect several antibiotics, including penicillin G, in goat milk at tolerance levels. However, LacTek CEF was excellent in detecting ceftiofur residue in goat milk.

ERRATIC FLARING OF BL LAC IN 2012–2013: MULTIWAVELENGTH OBSERVATIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wehrle, Ann E.; Grupe, Dirk; Jorstad, Svetlana G.

2016-01-10

BL Lac, the eponymous blazar, flared to historically high levels at millimeter, infrared, X-ray, and gamma-ray wavelengths in 2012. We present observations made with Herschel, Swift, NuSTAR, Fermi, the Submillimeter Array, CARMA, and the VLBA in 2012–2013, including three months with nearly daily sampling at several wavebands. We have also conducted an intensive campaign of 30 hr with every-orbit observations by Swift and NuSTAR, accompanied by Herschel, and Fermi observations. The source was highly variable at all bands. Time lags, correlations between bands, and the changing shapes of the spectral energy distributions can be explained by synchrotron radiation and inversemore » Compton emission from nonthermal seed photons originating from within the jet. The passage of four new superluminal very long baseline interferometry knots through the core and two stationary knots about 4 pc downstream accompanied the high flaring in 2012–2013. The seed photons for inverse Compton scattering may arise from the stationary knots and from a Mach disk near the core where relatively slow-moving plasma generates intense nonthermal radiation. The 95 spectral energy distributions obtained on consecutive days form the most densely sampled, broad wavelength coverage for any blazar. The observed spectral energy distributions and multi-waveband light curves are similar to simulated spectral energy distributions and light curves generated with a model in which turbulent plasma crosses a conical shock with a Mach disk.« less

EGRET observations of the BL Lacertae objects 0716+714 and 0521-365

NASA Technical Reports Server (NTRS)

Lin, Y. C.; Bertsch, D. L.; Dingus, B. L.; Esposito, J. A.; Fichtel, C. E.; Hartman, R. C.; Hunter, S. D.; Kanbach, G.; Kniffen, D. A.; Mayer-Hasselwander, H. A.

1995-01-01

During the Compton Observatory's viewing programs Phase 1 (1991 April to 1992 November, also known as the All-Sky Survey) and Phase 2 (1992 November to 1993 September), the BL Lac object 0716+714 was in the field of view of the EGRET telescope a total of six times, three times in Phase 1 and three more times in Phase 2, while the BL Lac object 0521-365 was in the field of view of EGRET only once in Phase 1. The source 0716+714 was detected in high-energy gamma rays by EGRET at a flux level of (2.0 +/- 0.4) x 10(exp -7) photons/sq cm/s for E greater than 100 MeV with a 6 sigma significance when it was first observed by EGRET in 1992 January 10 to 23. The corresponding spectral slope of the photon number distribution is determined to be -2.04 +/- 0.33. The gamma-ray flux of 0716+714 showed considerable time variability in subsequent EGRET observations. But the spectral slope stayed about the same within the statistical uncertainties of the EGRET data. The average spectral slope of the four viewing periods during which the photon flux of 0716+714 stayed above the EGRET detection threshold is found to be -1.85 +/- 0.20 from the combined data. The source 0521+365 was detected by EGRET in 1992 May 14 to June 4 at a flux level of (1.8 +/- 0.5) x 10(exp -7) photons/sq cm/s for E greater than 100 MeV with a 4 sigma significance. The corresponding spectral slope of the photon number distribution is found to be 2.16 +/- 0.36. Details of the observations of these two BL Lac objects with the EGRET telescope are presented.

Transcriptomic Analysis of Lung Tissue from Cigarette Smoke-Induced Emphysema Murine Models and Human Chronic Obstructive Pulmonary Disease Show Shared and Distinct Pathways.

PubMed

Yun, Jeong H; Morrow, Jarrett; Owen, Caroline A; Qiu, Weiliang; Glass, Kimberly; Lao, Taotao; Jiang, Zhiqiang; Perrella, Mark A; Silverman, Edwin K; Zhou, Xiaobo; Hersh, Craig P

2017-07-01

Although cigarette smoke (CS) is the primary risk factor for chronic obstructive pulmonary disease (COPD), the underlying molecular mechanisms for the significant variability in developing COPD in response to CS are incompletely understood. We performed lung gene expression profiling of two different wild-type murine strains (C57BL/6 and NZW/LacJ) and two genetic models with mutations in COPD genome-wide association study genes (HHIP and FAM13A) after 6 months of chronic CS exposure and compared the results to human COPD lung tissues. We identified gene expression patterns that correlate with severity of emphysema in murine and human lungs. Xenobiotic metabolism and nuclear erythroid 2-related factor 2-mediated oxidative stress response were commonly regulated molecular response patterns in C57BL/6, Hhip +/- , and Fam13a -/- murine strains exposed chronically to CS. The CS-resistant Fam13a -/- mouse and NZW/LacJ strain revealed gene expression response pattern differences. The Fam13a -/- strain diverged in gene expression compared with C57BL/6 control only after CS exposure. However, the NZW/LacJ strain had a unique baseline expression pattern, enriched for nuclear erythroid 2-related factor 2-mediated oxidative stress response and xenobiotic metabolism, and converged to a gene expression pattern similar to the more susceptible wild-type C57BL/6 after CS exposure. These results suggest that distinct molecular pathways may account for resistance to emphysema. Surprisingly, there were few genes commonly modulated in mice and humans. Our study suggests that gene expression responses to CS may be largely species and model dependent, yet shared pathways could provide biologically significant insights underlying individual susceptibility to CS.

VizieR Online Data Catalog: SPIRE observations of Herschel-BAT sample (Shimizu+, 2016)

NASA Astrophysics Data System (ADS)

Shimizu, T. T.; Melendez, M.; Mushotzky, R. F.; Koss, M. J.; Barger, A. J.; Cowie, L. L.

2016-09-01

We selected our sample of 313 AGN from the 58 month Swift/BAT Catalogue (https://swift.gsfc.nasa.gov/results/bs58mon) (Baumgartner et al., 2012, in prep.), imposing a redshift cutoff of z<0.05. All different types of AGN were chosen only excluding Blazars/BL Lac objects which most likely introduce complicated beaming effects. To determine their AGN type, for 252 sources we used the classifications from the BAT AGN Spectroscopic Survey (Koss et al., in preparation) which compiled and analysed optical spectra for the Swift/BAT 70 month catalogue (Berney et al., 2015MNRAS.454.3622B). (2 data files).

Extraordinary Activity in the BL Lac Object OJ 287

NASA Astrophysics Data System (ADS)

Hughes, P. A.; Aller, H. D.; Aller, M. F.

We present the results of a wavelet transform analysis of data for the BL Lac object OJ 287 acquired as part of the UMRAO variability program. We find clear evidence for a persistent modulation of the total flux and polarization with period 1.66 years, and for another signal that dominates activity in the 1980s with period 1.12 years. It appears that the longer time scale periodicity is associated with an otherwise quiescent jet, and the shorter time scale activity is associated with the passage of a shock, or shocks. The periodic behavior in polarization exhibits excursions in U which correspond to a direction 45circ from the VLBI jet axis. This behavior suggests a small amplitude, cyclic variation in the flow direction in that part of the flow that dominates cm-wavelength emission.

Optical and X-ray observations of the two BL Lac objects OJ 287 and MS 1458+22

NASA Astrophysics Data System (ADS)

Massaro, E.; Giommi, P.; Perri, M.; Tagliaferri, G.; Nesci, R.; Tosti, G.; Ciprini, S.; Montagni, F.; Ravasio, M.; Ghisellini, G.; Frasca, A.; Marilli, E.; Valentini, G.; Kurtanidze, O. M.; Nikolashvili, M. G.

2003-02-01

We present the results of recent BeppoSAX observations of the two BL Lac objects OJ 287 and MS 1458+22 in bright optical states and of simultaneous photometric measurements in the bandpasses from I to U. OJ 287 was observed in November 2001 and MS 1458+22 in February of the same year. The X-ray flux of OJ 287 was rather low, F(2-10 keV) = (1.35+/-0.15)x 10-12 erg cm-2 s-1 and its spectrum is well described by a single power law with an energy index 0.45+/-0.08, flatter than that measured in the optical, equal to 1.53+/-0.03. The X-ray emission of MS 1458+22 was characterized by a low flux level of F(2-10 keV) = (7.5+/-0.6)x 10-13 erg cm-2 s-1, with a steeper spectral index of 1.71+/-0.15, while in the optical it was 0.99+/-0.06. We show that the broad-band SEDs of these two sources can be well represented by log-parabolic spectral laws and evaluate their parameters. This law, already observed for the synchrotron components of other BL Lac sources, can be explained if the emitting particles are accelerated by some statistical mechanism having a probability of energy gain that is a decreasing function of the energy itself.

Discovery of VHE γ -ray emission from the BL Lacertae object B3 2247+381 with the MAGIC telescopes

DOE PAGES

Aleksić, J.; Alvarez, E. A.; Antonelli, L. A.; ...

2012-03-02

Here, we study the non-thermal jet emission of the BL Lac object B3 2247+381 during a high optical state. The MAGIC telescopes observed the source during 13 nights between September 30th and October 30th 2010, collecting a total of 14.2 h of good quality very high energy (VHE) γ-ray data. Simultaneous multiwavelength data was obtained with X-ray observations by the Swift satellite and optical R-band observations at the KVA-telescope. We also use high energy γ-ray (HE, 0.1-100 GeV) data from the Fermi satellite. We also dedected the BL Lac object B3 2247+381 (z = 0.119) , for the first time,more » at VHE γ-rays at a statistical significance of 5.6σ. A soft VHE spectrum with a photon index of -3.2 ± 0.6 was determined. No significant short term flux variations were found. Finally, we model the spectral energy distribution using a one-zone SSC-model, which can successfully describe our data.« less

Optical Variability of BL Lacertae During the Major Outburst of 1997

NASA Technical Reports Server (NTRS)

Ghosh, K. K.; Ramsey, B. D.; Sadun, A. C.; Soundararajaperumal, S.; Wang, J.

2000-01-01

We have undertaken an investigation of recent flux variability in BL Lac. We present optical observations taken over 22 nights documenting major as well as minor outbursts. This has been combined, for purposes of multifrequency analysis, with published X-ray and T-ray data taken for an additional single night, On two nights in particular, including the night of the X-ray observations, a major outburst of about a full magnitude of variation was recorded. All the data have been analyzed with theoretical models. Attempts were made to use synchrotron self-Compton and external Comptonization models to explain the data; however, both classes of models were found lacking. More satisfactory results were obtained using an analytical model proposed by Wang et al. that involves the evolution of synchrotron spectra in a homogeneous jet due to the injection of relativistic electrons, taking into account radiation losses during the outbursts. It is hoped that the results of this study of BL Lac, an archetype for the class of blazars in general, represent a more generic phenomenon applicable to the entire class.

Optical Variability of BL Lacertae During the Major Outburst of 1997

NASA Technical Reports Server (NTRS)

Ghosh, K. K.; Ramsey, B. D.; Sadun, A. C.; Soundarajaperumal, S.; Wang, J.

1999-01-01

We have undertaken an investigation of recent flux variability in BL Lac. We present optical observations taken over 12 nights documenting major as well as minor outbursts. This has been combined, for purposes of multifrequency analysis, with published X-ray and gamma ray data taken for an additional single night. On two nights in particular, which includes the night of the X-ray observations, a major outburst of about a full magnitude of variation was recorded. All the data have been analyzed with theoretical models. Attempts were made to use synchrotron self-Compton and external Comptonization models to explain the data; however both classes of models were found lacking. More satisfactory results were obtained using an analytical model proposed by Wang et al. that involves the evolution of synchrotron spectra in a homogeneous jet due to the injection of relativistic electrons, taking into account radiation losses during the outbursts. It is hoped that the results of this study of BL Lac, an archetype for the class of blazars in general, represent a more generic phenomenon applicable to the entire class.

Simultaneous Planck , Swift , and Fermi observations of X-ray and γ -ray selected blazars

DOE PAGES

Giommi, P.; Polenta, G.; Lähteenmäki, A.; ...

2012-05-22

We present simultaneous Planck, Swift, Fermi, and ground-based data for 105 blazars belonging to three samples with flux limits in the soft X-ray, hard X-ray, and γ-ray bands, with additional 5GHz flux-density limits to ensure a good probability of a Planck detection. We compare our results to those of a companion paper presenting simultaneous Planck and multi-frequency observations of 104 radio-loud northern active galactic nuclei selected at radio frequencies. While we confirm several previous results, our unique data set allows us to demonstrate that the selection method strongly influences the results, producing biases that cannot be ignored. Almost all themore » BL Lac objects have been detected by the Fermi Large AreaTelescope (LAT), whereas 30% to 40% of the flat-spectrum radio quasars (FSRQs) in the radio, soft X-ray, and hard X-ray selected samples are still below the γ-ray detection limit even after integrating 27 months of Fermi-LAT data. The radio to sub-millimetre spectral slope of blazars is quite flat, with >α> ~ 0 up to about 70GHz, above which it steepens to ~ -0.65. The BL Lacs have significantly flatter spectra than FSRQs at higher frequencies. The distribution of the rest-frame synchrotron peak frequency (ν peak S) in the spectral energy distribution (SED) of FSRQs is the same in all the blazar samples with peak S> = 10 13.1 ± 0.1 Hz, while the mean inverse Compton peak frequency, >ν peak IC>, ranges from 1021 to 1022 Hz. The distributions of ν peak S and ν peak IC of BL Lacs are much broader and are shifted to higher energies than those of FSRQs; their shapes strongly depend on the selection method. The Compton dominance of blazars, defined as the ratio of the inverse Compton to synchrotron peak luminosities, ranges from less than 0.2 to nearly 100, with only FSRQs reaching values larger than about 3. Its distribution is broad and depends strongly on the selection method, with γ-ray selected blazars peaking at ~7 or more, and radio-selected blazars at values close to 1, thus implying that the common assumption that the blazar power budget is largely dominated by high-energy emission is a selection effect. A comparison of our multi-frequency data with theoretical predictions shows that simple homogeneous SSC models cannot explain the simultaneous SEDs of most of the γ-ray detected blazars in all samples. The SED of the blazars that were not detected by Fermi-LAT may instead be consistent with SSC emission. Our data challenge the correlation between bolometric luminosity and ν peak S predicted by the blazar sequence.« less

Cloning, Purification, and Characterization of a Heterodimeric β-Galactosidase from Lactobacillus kefiranofaciens ZW3.

PubMed

He, Xi; Han, Ning; Wang, Yan-Ping

2016-01-01

Lactobacillus kefiranofaciens ZW3 was obtained from kefir grains, which have high lactose hydrolytic activity. In this study, a heterodimeric LacLM-type β-galactosidase gene (lacLM) from ZW3 was isolated, which was composed of two overlapping genes, lacL (1,884 bp) and lacM (960 bp) encoding large and small subunits with calculated molecular masses of 73,620 and 35,682 Da, respectively. LacLM, LacL, and LacM were expressed in Escherichia coli BL21(DE3) and these recombinant proteins were purified and characterized. The results showed that, compared with the recombinant holoenzyme, the recombinant large subunit exhibits obviously lower thermostability and hydrolytic activity. Moreover, the optimal temperature and pH of the holoenzyme and large subunit are 60°C and 7.0, and 50°C and 8.0, respectively. However, the recombinant small subunit alone has no activity. Interestingly, the activity and thermostability of the large subunit were greatly improved after mixing it with the recombinant small subunit. Therefore, the results suggest that the small subunit might play an important role in maintaining the stability of the structure of the catalytic center located in the large subunit.

Spectroscopy of 10 γ -Ray BL Lac Objects at High Redshift

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paiano, Simona; Falomo, Renato; Landoni, Marco

2017-08-01

We present optical spectra with high signal-to-noise ratio of 10 BL Lac objects detected at GeV energies by the Fermi satellite (3FGL catalog), which previous observations suggested are at relatively high redshift. The new observations, obtained at the 10 m Gran Telescopio Canarias, allowed us to find the redshift for J0814.5+2943 ( z = 0.703), and we can set a spectroscopic lower limit for J0008.0+4713 ( z > 1.659) and J1107.7+0222 ( z > 1.0735) on the basis of Mg ii intervening absorption features. In addition we confirm the redshifts for J0505.5+0416 ( z = 0.423) and J1450+5200 ( zmore » > 2.470). Finally we contradict the previous z estimates for five objects (J0049.7+0237, J0243.5+7119, J0802.0+1005, J1109.4+2411, and J2116.1+3339).« less

VizieR Online Data Catalog: Swift and NuSTAR obs. of the BL Lac Mrk 421 (Kapanadze+, 2016)

NASA Astrophysics Data System (ADS)

Kapanadze, B.; Dorner, D.; Vercellone, S.; Romano, P.; Aller, H.; Aller, M.; Hughes, P.; Reynolds, M.; Kapanadze, S.; Tabagari, L.

2017-01-01

We retrieved the Swift-XRT data from the publicly available archive, maintained by HEASARC. We present the results of X-ray observations of the high-energy peaked BL Lac (HBL) source Mrk421 performed by Swift-XRT and NuSTAR during 2013 January-June. Along with the 0.3-10keV and 3-79keV data obtained with the Swift-XRT and NuSTAR instruments, we have processed and analyzed those obtained with the Ultraviolet-Optical Telescope (UVOT) and Large Area Telescope (LAT) onboard Fermi. We have also used the publicly available light curves from the observations performed with the Burst Alert Telescope (BAT) onboard Swift, Monitor of All Sky X-ray Image (MAXI), MAGIC, First G-APD Cherenkov Telescope (FACT), and the OVRO 40m telescope during the 2013 January-June period to draw conclusions about the interband correlations. (8 data files).

A hadronic origin for ultra-high-frequency-peaked BL Lac objects

NASA Astrophysics Data System (ADS)

Cerruti, M.; Zech, A.; Boisson, C.; Inoue, S.

2015-03-01

Current Cherenkov telescopes have identified a population of ultra-high-frequency peaked BL Lac objects (UHBLs), also known as extreme blazars, that exhibit exceptionally hard TeV spectra, including 1ES 0229+200, 1ES 0347-121, RGB J0710+591, 1ES 1101-232, and 1ES 1218+304. Although one-zone synchrotron-self-Compton (SSC) models have been generally successful in interpreting the high-energy emission observed in other BL Lac objects, they are problematic for UHBLs, necessitating very large Doppler factors and/or extremely high minimum Lorentz factors of the emitting leptonic population. In this context, we have investigated alternative scenarios where hadronic emission processes are important, using a newly developed (lepto-)hadronic numerical code to systematically explore the physical parameters of the emission region that reproduces the observed spectra while avoiding the extreme values encountered in pure SSC models. Assuming a fixed Doppler factor δ = 30, two principal parameter regimes are identified, where the high-energy emission is due to: (1) proton-synchrotron radiation, with magnetic fields B ˜ 1-100 G and maximum proton energies Ep; max ≲ 1019 eV; and (2) synchrotron emission from p-γ-induced cascades as well as SSC emission from primary leptons, with B ˜ 0.1-1 G and Ep; max ≲ 1017 eV. This can be realized with plausible, sub-Eddington values for the total (kinetic plus magnetic) power of the emitting plasma, in contrast to hadronic interpretations for other blazar classes that often warrant highly super-Eddington values.

The NuSTAR view on Hard-TeV BL Lacs

NASA Astrophysics Data System (ADS)

Costamante, L.; Bonnoli, G.; Tavecchio, F.; Ghisellini, G.; Tagliaferri, G.; Khangulyan, D.

2018-05-01

Hard-TeV BL Lacs are a new type of blazars characterized by a hard intrinsic TeV spectrum, locating the peak of their gamma-ray emission in the spectral energy distribution (SED) above 2-10 TeV. Such high energies are problematic for the Compton emission, using a standard one-zone leptonic model. We study six examples of this new type of BL Lacs in the hard X-ray band with NuSTAR. Together with simultaneous observations with the Neil Gehrels Swift Observatory, we fully constrain the peak of the synchrotron emission in their SED, and test the leptonic synchrotron self-Compton (SSC) model. We confirm the extreme nature of 5 objects also in the synchrotron emission. We do not find evidence of additional emission components in the hard X-ray band. We find that a one-zone SSC model can in principle reproduce the extreme properties of both peaks in the SED, from X-ray up to TeV energies, but at the cost of i) extreme electron energies with very low radiative efficiency, ii) conditions heavily out of equipartition (by 3 to 5 orders of magnitude), and iii) not accounting for the simultaneous UV data, which then should belong to a different emission component, possibly the same as the far-IR (WISE) data. We find evidence of this separation of the UV and X-ray emission in at least two objects. In any case, the TeV electrons must not "see" the UV or lower-energy photons, even if coming from different zones/populations, or the increased radiative cooling would steepen the VHE spectrum.

The NuSTAR view on hard-TeV BL Lacs

NASA Astrophysics Data System (ADS)

Costamante, L.; Bonnoli, G.; Tavecchio, F.; Ghisellini, G.; Tagliaferri, G.; Khangulyan, D.

2018-07-01

Hard-TeV BL Lacs are a new type of blazars characterized by a hard intrinsic TeV spectrum, locating the peak of their gamma-ray emission in the spectral energy distribution (SED) above 2-10 TeV. Such high energies are problematic for the Compton emission, using a standard one-zone leptonic model. We study six examples of this new type of BL Lacs in the hard X-ray band with NuSTAR. Together with simultaneous observations with the Neil Gehrels Swift Observatory, we fully constrain the peak of the synchrotron emission in their SED, and test the leptonic synchrotron self-Compton (SSC) model. We confirm the extreme nature of five objects also in the synchrotron emission. We do not find evidence of additional emission components in the hard X-ray band. We find that a one-zone SSC model can in principle reproduce the extreme properties of both peaks in the SED, from X-ray up to TeV energies, but at the cost of (i) extreme electron energies with very low radiative efficiency, (ii) conditions heavily out of equipartition (by three to five orders of magnitude), and (iii) not accounting for the simultaneous UV data, which then should belong to a different emission component, possibly the same as the far-IR (WISE) data. We find evidence of this separation of the UV and X-ray emission in at least two objects. In any case, the TeV electrons must not `see' the UV or lower energy photons, even if coming from different zones/populations, or the increased radiative cooling would steepen the very high energies spectrum.

Suzaku observations of γ-ray bright radio galaxies: Origin of the x-ray emission and broadband modeling

DOE PAGES

Fukazawa, Yasushi; Finke, Justin; Stawarz, Łukasz; ...

2014-12-24

Here, we performed a systematic X-ray study of eight nearby γ-ray bright radio galaxies with Suzaku in order to understand the origins of their X-ray emissions. The Suzaku spectra for five of those have been presented previously, while the remaining three (M87, PKS 0625–354, and 3C 78) are presented here for the first time. Based on the Fe-K line strength, X-ray variability, and X-ray power-law photon indices, and using additional information on the [O III] line emission, we argue for a jet origin of the observed X-ray emission in these three sources. We also analyzed five years of Fermi Largemore » Area Telescope (LAT) GeV gamma-ray data on PKS 0625–354 and 3C 78 to understand these sources within the blazar paradigm. We found significant γ-ray variability in the former object. Overall, we note that the Suzaku spectra for both PKS 0625–354 and 3C 78 are rather soft, while the LAT spectra are unusually hard when compared with other γ-ray detected low-power (FR I) radio galaxies. We demonstrate that the constructed broadband spectral energy distributions of PKS 0625–354 and 3C 78 are well described by a one-zone synchrotron/synchrotron self-Compton model. The results of the modeling indicate lower bulk Lorentz factors compared to those typically found in other BL Lacertae (BL Lac) objects, but consistent with the values inferred from modeling other LAT-detected FR I radio galaxies. Interestingly, the modeling also implies very high peak (~10 16 Hz) synchrotron frequencies in the two analyzed sources, contrary to previously suggested scenarios for Fanaroff-Riley (FR) type I/BL Lac unification. Finally, we discuss the implications of our findings in the context of the FR I/BL Lac unification schemes.« less

A physical classification scheme for blazars

NASA Astrophysics Data System (ADS)

Landt, Hermine; Padovani, Paolo; Perlman, Eric S.; Giommi, Paolo

2004-06-01

Blazars are currently separated into BL Lacertae objects (BL Lacs) and flat spectrum radio quasars based on the strength of their emission lines. This is performed rather arbitrarily by defining a diagonal line in the Ca H&K break value-equivalent width plane, following Marchã et al. We readdress this problem and put the classification scheme for blazars on firm physical grounds. We study ~100 blazars and radio galaxies from the Deep X-ray Radio Blazar Survey (DXRBS) and 2-Jy radio survey and find a significant bimodality for the narrow emission line [OIII]λ5007. This suggests the presence of two physically distinct classes of radio-loud active galactic nuclei (AGN). We show that all radio-loud AGN, blazars and radio galaxies, can be effectively separated into weak- and strong-lined sources using the [OIII]λ5007-[OII]λ3727 equivalent width plane. This plane allows one to disentangle orientation effects from intrinsic variations in radio-loud AGN. Based on DXRBS, the strongly beamed sources of the new class of weak-lined radio-loud AGN are made up of BL Lacs at the ~75 per cent level, whereas those of the strong-lined radio-loud AGN include mostly (~97 per cent) quasars.

The Study on the Physical Properties of Blazar Jets

NASA Astrophysics Data System (ADS)

Kang, S. J.

2017-09-01

Active galactic nuclei (AGNs) belong to a special class of active galaxies, and have violent active phenomena and intense physical processes in the nuclei. Blazar is a subclass of AGNs, and has a relativistic jet with a small jet viewing angle. Therefore, the boosting effect is very important, and almost all the observed radiation is dominated by the jet. The relativistic jet physics is not very clear yet, such as the jet formation, collimation, and matter content etc. The multi-waveband radiation of blazar is dominated by jet, which provides an ideal laboratory for studying the jet physics. The first chapter of this thesis introduces the recent progress of AGNs and blazars. We further introduce the jet model that commonly used in blazars in the second chapter. In the third chapter, we fit simultaneously (or quasi-simultaneously) the multi-waveband spectral energy distributions (SEDs) for a sample of low-synchrotron-peaked (LSP) blazars with the jet model and χ2 procedure, which takes into account different soft photon fields (broad line region or a molecular torus). We find that the SED fitting with an external soft photon from IR torus is systematically better than that from the broad line region (BLR) based on a χ2 test, which suggests that the γ-ray emitting region most possibly stays outside the BLR. The minimum electron Lorentz factor, γmin, is constrained from the modeling of these LSP blazars with good soft X-ray data, and in a range from 5 to 160 (with a median value of 55), which plays a key role in jet power estimation. Assuming one-to-one ratio of proton and electron, we find that the jet power for LSP blazars is systematically higher than that of Fanaroff-Riley type II (FR II) radio galaxies. A possible reason for this is that there are some positrons in the jets of these blazars. If this is the case, the jet power will be reduced. Therefore, we propose a mixed composition of e±-p in the jets of these LSP blazars. If we assume that the jet power of LSP blazars is the same as that of FR IIs, we find that it is an electron-positron pair dominated leptonic jet in these blazars, and the number density of electron-positron pairs is several times higher than that of electron-proton pairs, but the jet power is still dominated by protons. For the high-synchrotron-peaked (HSP) BL Lac PKS 1424+240, the SED fitting with the synchrotron self-Compton (SSC) model gave unreasonable fitting parameters (e.g., a very large Doppler factor δ). In this work, we take into account the possible external soft photon field, and then fit the multi-waveband SEDs of blazar PKS 1424+240 with one-zone leptonic jet models in both states. We find the SSC+external-Compton (EC) model can give a better fitting result if the EC process is included. However, the needed energy density of external soft photon field (U_{ext}) is much lower than the typical value. This result is consistent with the results of some other BL Lacs, where the BLR or torus is very weak or disappearing. It means that there is evolution of the energy density of external soft photon field with decreasing of the luminosity of blazars (the flat spectrum radio quasars (FSRQs)-BL Lac: low energy peaked BL Lac (LBL)-intermediate energy peaked BL Lac (IBL)-high energy peaked BL Lac (HBL)). And on this basis, in the chapter 5, we further explore the possible evolution of the external soft photon field of blazars based on the EC process. We employ the one-zone homogeneous leptonic jet model and χ2 procedure to fit simultaneously or quasi-simultaneously multi-waveband SEDs for a sample of blazars with a wide distribution of luminosities. In our model, we set Uext as a free parameter. Studying the energy density of the external photon field in different subclasses of blazars, we find: (1) the Uext of the high luminosity blazar (FSRQs and LBLs) keeps roughly as a constant, which is, however, smaller than that constrained from BLR observations. Assuming IR as the source of soft photons, the Uext is roughly consistent with the torus observational result. This further supports the result that the external soft photon field may originate from torus, and the γ-ray emitting region of these LSP blazars locates outside the BLR. (2) For some IBLs, the EC process may be still needed, but the photon energy density is less than the typical values of the photon energy density of BLR (or dust torus), where the Uext decreases with decreasing of the luminosity. This evolution is consistent with the BLR or torus as directly constrained from the radio-quiet AGN. The final part summarizes the study on the subject, and makes some suggestions for further researches.

Three new BL Lacertae objects in the Palomar-Green survey

NASA Technical Reports Server (NTRS)

Fleming, Thomas A.; Green, Richard F.; Jannuzi, Buell T.; Liebert, James; Smith, Paul S.; Fink, Henner

1993-01-01

We have identified three BL Lacertae objects in the Palomar-Green Survey which were previously misclassified as DC white dwarfs, namely PG 1246+586, PG 1424+240, and PG 1437+398. Our reclassification is based on the detection of these objects as x-ray sources in the ROSAT all-sky survey and upon our subsequent detection of intrinsic linearly polarized and variable optical emission from these sources. As a result of the ROSAT survey, the number of identified BL Lac objects in the Palomar-Green catalog of UV excess objects has been doubled. Corrected optical positions are presented for PG 1246+586 and PG 1437+398.

Simultaneous Planck, Swift, and Fermi Observations of X-ray and Gamma-ray Selected Blazars

NASA Technical Reports Server (NTRS)

Giommi, P.; Polenta, G.; Laehteenmaeki, A.; Thompson, D. J.; Capalbi, M.; Cutini, S.; Gasparrini, D.; Gonzalez, Nuevo, J.; Leon-Tavares, J.; Lopez-Caniego, M.;

Understanding nature's particle accelerators using high energy gamma-ray survey instruments

NASA Astrophysics Data System (ADS)

Abeysekara, Anushka Udara

Nature's particle accelerators, such as Pulsars, Pulsar Wind Nebulae, Active Galactic Nuclei and Supernova Remnants accelerate charged particles to very high energies that then produce high energy photons. The particle acceleration mechanisms and the high energy photon emission mechanisms are poorly understood phenomena. These mechanisms can be understood either by studying individual sources in detail or, alternatively, using the collective properties of a sample of sources. Recent development of GeV survey instruments, such as Fermi-LAT, and TeV survey instruments, such as Milagro, provides a large sample of high energy gamma-ray flux measurements from galactic and extra-galactic sources. In this thesis I provide constraints on GeV and TeV radiation mechanisms using the X-ray-TeV correlations and GeV-TeV correlations. My data sample was obtained from three targeted searches for extragalactic sources and two targeted search for galactic sources, using the existing Milagro sky maps. The first extragalactic candidate list consists of Fermi-LAT GeV extragalactic sources, and the second extragalactic candidate list consists of TeVCat extragalactic sources that have been detected by Imaging Atmospheric Cerenkov Telescopes (IACTs). In both extragalactic candidate lists Markarian 421 was the only source detected by Milagro. A comparison between the Markarian 421 time-averaged flux, measured by Milagro, and the flux measurements of transient states, measured by IACTs, is discussed. The third extragalactic candidate list is a list of potential TeV emitting BL Lac candidates that was synthesized using X-ray observations of BL Lac objects and a Synchrotron Self-Compton model. Milagro's sensitivity was not sufficient to detect any of those candidates. However, the 95% confidence flux upper limits of those sources were above the predicted flux. Therefore, these results provide evidence to conclude that the Synchrotron Self-Compton model for BL Lac objects is still a viable model. Targeted searches for galactic candidates were able to measure TeV emission associated with 14 Fermi-LAT GeV pulsars. In this thesis I also presented a new multi-wavelength technique that I developed to isolate the flux correlation factor (fΩ ) of pulsars as a function of pulsar spin down luminosity. The correlation between fΩ and pulsar spin-down luminosity for a Fermi-LAT GeV pulsar sample was measured using the measurements obtained in the Milagro targeted search performed for galactic sources and from the literature. The measured correlation has some features that favor the Outer Gap model over the Polar Cap, Slot Gap and One Pole Caustic models for pulsar emission in the energy range of 0.1 to 100 GeV. However, these simulated models failed to explain many other important pulsar population characteristics. Therefore, further improvements on the galactic pulsar population simulations are needed to provide tighter constraints.

Simultaneous Planck, Swift, and Fermi Observations of X-Ray and gamma-Ray Selected Blazars

NASA Technical Reports Server (NTRS)

Giommi, P.; Polenta, G.; Laehteenmaeki, A.; Thompson, D. J.; Capalbi, M.; Cutini, S.; Gasparrini, D.; Gonzalez-Nuevo, J.; Leon-Tavares, J.; Lopez-Caniego, M.;

Does the Blazar Gamma-ray Spectrum Harden with Increasing Flux? - Analysis of Nine Years of EGRET Data

NASA Technical Reports Server (NTRS)

Nandikotkur, Giridhar; Jahoda, Keith M.; Hartman, R. C.; Mukherjee, R.; Sreekumar, P.; Boettcher, M.

2007-01-01

The Energetic Gamma Ray Experiment Telescope (EGRET) on the Compton Gamma Ray Observatory (CGRO) discovered gamma-ray emission from more than 67 blazars during its nine-year lifetime. We conducted an exhaustive search of the EGRET archives and selected all the blazars that were observed multiple times and were bright enough to enable a spectral analysis using standard powerlaw models. The sample consists of 18 flat-spectrum radio quasars (FSRQs), 6 low-frequency-peaked BL Lacs (LBLs) and 2 high-frequency-peaked BL Lacs (HBLs). We do not detect any clear pattern in'the variation of spectral index with flux. Some of the blazars do not show any statistical evidence for spectral variability. The spectrum hardens with increasing flux in a few cases. There is also evidence for a flux-hardness anticorrelation at lo\\v fluxes in five blazars. The well observed blazars (3C 279,3C 273, PKS 0528-i-134, PKS 1622-297, PKS 0208- 512) do not show any overall trend in the long-term spectral dependence on flux, but the sample shows a mixture of hard and soft states. We observed spectral hysteresis at weekly timescales in all the three FSRQs for which data from flares lasting for 3 approx. 4 weeks were available. All three sources show a counterclockwise rotation despite the widely different flux profiles. Hysteresis in the spectral index vs. flux space has never been observed in FSRQs in gamma-rays at weekly timescales. itre analyze the observed spectral behavior in the context of various inverse-Compton mechanisms believed to be responsible for emission in the EGRET energy range. Our analysis uses the EGRET skymaps that were regenerated to include the changes in performance during the mission.

HESS J1943+213: A Non-classical High-frequency-peaked BL Lac Object

NASA Astrophysics Data System (ADS)

Straal, S. M.; Gabányi, K. É.; van Leeuwen, J.; Clarke, T. E.; Dubner, G.; Frey, S.; Giacani, E.; Paragi, Z.

2016-05-01

HESS J1943+213 is an unidentified TeV source that is likely a high-frequency-peaked BL Lac (HBL) object, but that is also compatible with a pulsar wind nebula (PWN) nature. Each of these enormously different astronomical interpretations is supported by some of the observed unusual characteristics. In order to finally classify and understand this object, we took a three-pronged approach, through time-domain, high angular resolution, and multi-frequency radio studies. First, our deep time-domain observations with the Arecibo telescope failed to uncover the putative pulsar powering the proposed PWN. We conclude with ˜70% certainty that HESS J1943+213 does not host a pulsar. Second, long-baseline interferometry of the source with e-MERLIN at 1.5 and 5 GHz shows only a core, that is, a point source at ˜ 1-100 mas resolution. Its 2013 flux density is about one-third lower than that detected in the 2011 observations with similar resolution. This radio variability of the core strengthens the HBL object hypothesis. Third, additional evidence against the PWN scenario comes from the radio spectrum we compiled. The extended structure follows a power-law behavior with spectral index α \\=\\-0.54+/- 0.04 while the core component displays a flat spectrum (α \\=\\-0.03+/- 0.03). In contrast, the radio synchrotron emission of PWNe predicts a single power-law distribution. Overall, we rule out the PWN hypothesis and conclude that the source is a BL Lac object. The consistently high fraction (70%) of the flux density from the extended structure then leads us to conclude that HESS J1943+213 must be a non-classical HBL object.

Multiwavelength Observations Of The Previously Unidentified Blazar RX J0648.7+1516

DOE PAGES

Aliu, E.

2011-11-15

We report on the VERITAS discovery of very-high-energy (VHE) gammaray emission above 200 GeV from the high-frequency-peaked BL Lac object RXJ0648.7+1516 (GBJ0648+1516), associated with 1FGL J0648.8+1516. The photon spectrum above 200 GeV is fit by a power law dN/dE = F0(E/E0) -Γ with a photon index Γ of 4.4 ± 0.8stat ± 0.3syst and a flux normalization F0 of (2.3±0.5stat ±1.2sys)×10 -11 TeV -1cm -2s -1 with E0 = 300 GeV. No VHE variability is detected during VERITAS observations of RXJ0648.7+1516 between 2010 March 4 and April 15. Following the VHE discovery, the optical identification and spectroscopic redshift were obtainedmore » using the Shane 3–m Telescope at the Lick Observatory, showing the unidentified object to be a BL Lac type with a redshift of z = 0.179. Broadband multiwavelength observations contemporaneous with the VERITAS exposure period can be used to sub-classify the blazar as a high-frequency-peaked BL Lac (HBL) object, including data from the MDM observatory, Swift -UVOT and XRT, and continuous monitoring at photon energies above 1 GeV from the Fermi Large Area Telescope (LAT). We find that in the absence of undetected, high-energy rapid variability, the one-zone synchrotron self-Compton model (SSC) overproduces the high-energy gamma-ray emission measured by the Fermi -LAT over 2.3 years. The SED can be parameterized satisfactorily with an external-Compton or lepto-hadronic model, which have two and six additional free parameters, respectively, compared to the one-zone SSC model.« less

A KPC-Scale X-Ray Jet in the BL Lac Source S5 2007+777

NASA Technical Reports Server (NTRS)

Sambruna, Rita M.; Donato, Davide; Cheung, C.C.; Tavecchio, F.; Maraschi, L.

2008-01-01

X-ray jets in AGN are commonly observed in FRII and FRI radiogalaxies, but rarely in BL Lacs, most probably due to their orientation close to the line of sight and the ensuing foreshortening effects. Only three BL Lacs are known so far to contain a kpc-scale X-ray jet. In this paper, we present the evidence for the existence of a fourth extended X-ray jet in the classical radio-selected source S5 2007+777, which for its hybrid FRI/II radio morphology has been classified as a HYMOR (HYbrid MOrphology Radio source). Our Chandra ACISS observations of this source revealed an X-ray counterpart to the 19"-long radio jet. Interestingly, the X-ray properties of the kpc-scale jet in S5 2007+777 are very similar to those observed in FRII jets. First, the X-ray morphology closely mirrors the radio one, with the X-rays being concentrated in the discrete radio knots. Second, the X-ray continuum of the jet/brightest knot is described by a very hard power law, with photon index gamma(sub x) approximately 1. Third, the optical upper limit from archival HST data implies a concave radio-to-X-ray SED. If the X-ray emission is attributed to IC/CMB with equipartition, strong beaming (delta= 13) is required, implying a very large scale (Mpc) jet. The beaming requirement can be somewhat relaxed assuming a magnetic field lower than equipartition. Alternatively, synchrotron emission from a second population of very high-energy electrons is viable. Comparison to other HYMOR jets detected with Chandra is discussed, as well as general implications for the origin of the FRI/II division.

The Gamma-Ray Bright BL Lac Object RX J1211+2242

NASA Technical Reports Server (NTRS)

Beckmann, V.; Favre, P.; Tavecchio, F.; Bussien, T.; Fliri, J.; Wolter, A.

2004-01-01

RX J1211+2242 is an optically faint (B approximately equal to 19.2mag) but X-ray bright (f2-10kev = 5 x l0(exp -12)erg per square centimeter per second) AGN, which has been shown to be a BL Lac object at redshift z = 0.455. The ROSAT X-ray, Calar Alto optical, and NVSS radio data suggest that the peak of the synchrotron emission of this object is at energies as high as several keV. BeppoSAX observations have been carried out simultaneously with optical observations in order to extend the coverage to higher energies. The new data indeed indicate a turn-over in the 2 - 10keV energy region. We propose that RX J1211+2242 is the counterpart of the unidentified EGRET source 3EG J1212+2304, making it a gamma-ray emitter with properties similar to, for example, Markarian 501 in its bright state, though being at a much larger distance.

Multiwavelength Observations of Markarian 421 During a TeV/X-Ray Flare

NASA Technical Reports Server (NTRS)

Bertsch, D. L.; Bruhweiler, F.; Macomb, D. J.; Cheng, K.-P.; Carter-Lewis, D. A.; Akerlof, C. W.; Aller, H. D.; Aller, M. F.; Buckley, J. H.; Cawley, M. F.

1995-01-01

A TeV flare from the BL Lac object Mrk 421 was detected in May of 1994 by the Whipple Observatory air Cherenkov experiment during which the flux above 250 GeV increased by nearly an order of magnitude over a 2-day period. Contemporaneous observations by ASCA showed the X-ray flux to be in a very high state. We present these results, combined with the first ever simultaneous or nearly simultaneous observations at GeV gamma-ray, UV, IR, mm, and radio energies for this nearest BL Lac object. While the GeV gamma-ray flux increased slightly, there is little evidence for variability comparable to that seen at TeV and X-ray energies. Other wavelengths show even less variability. This provides important constraints on the emission mechanisms at work. We present the multiwavelength spectrum of this gamma-ray blazar for both quiescent and flaring states and discuss the data in terms of current models of blazar emission.

VizieR Online Data Catalog: Blazars equivalent widths and radio luminosity (Landt+, 2004)

NASA Astrophysics Data System (ADS)

Landt, H.; Padovani, P.; Perlman, E. S.; Giommi, P.

2004-07-01

Blazars are currently separated into BL Lacertae objects (BL Lacs) and flat spectrum radio quasars based on the strength of their emission lines. This is performed rather arbitrarily by defining a diagonal line in the Ca H&K break value-equivalent width plane, following Marcha et al. (1996MNRAS.281..425M). We readdress this problem and put the classification scheme for blazars on firm physical grounds. We study ~100 blazars and radio galaxies from the Deep X-ray Radio Blazar Survey (DXRBS, Cat. and ) and 2-Jy radio survey and find a significant bimodality for the narrow emission line [OIII]{lambda}5007. This suggests the presence of two physically distinct classes of radio-loud active galactic nuclei (AGN). We show that all radio-loud AGN, blazars and radio galaxies, can be effectively separated into weak- and strong-lined sources using the [OIII]{lambda}5007-[OII]{lambda}3727 equivalent width plane. This plane allows one to disentangle orientation effects from intrinsic variations in radio-loud AGN. Based on DXRBS, the strongly beamed sources of the new class of weak-lined radio-loud AGN are made up of BL Lacs at the ~75 per cent level, whereas those of the strong-lined radio-loud AGN include mostly (~97 per cent) quasars. (4 data files).

Mutation Spectrum Induced by Conazole Fungicides in LacI Transgenic C57BL/6 Mouse Liver.

EPA Science Inventory

Conazoles are antifungal agents used in both agricultural and pharmaceutical settings. Some conazoles, including propiconazole and triadimefon, induce hepatocellular tumors in mice, while other conazoles do not. We reported in a previous study that both propiconazole and triadime...

Gamma-Ray Light Curves And Variability Of Bright Fermi -Detected Blazars

DOE PAGES

Abdo, A. A.

2010-09-22

This paper presents light curves as well as the first systematic characterization of variability of the 106 objects in the high-confidence Fermi Large Area Telescope Bright AGN Sample (LBAS). Weekly light curves of this sample, obtained during the first 11 months of the Fermi survey (2008 August 4-2009 July 4), are tested for variability and their properties are quantified through autocorrelation function and structure function analysis. For the brightest sources, 3 or 4 day binned light curves are extracted in order to determine power density spectra (PDSs) and to fit the temporal structure of major flares. More than 50% ofmore » the sources are found to be variable with high significance, where high states do not exceed 1/4 of the total observation range. Variation amplitudes are larger for flat spectrum radio quasars and low/intermediate synchrotron frequency peaked BL Lac objects. Autocorrelation timescales derived from weekly light curves vary from four to a dozen of weeks. Variable sources of the sample have weekly and 3-4 day bin light curves that can be described by 1/f α PDS, and show two kinds of gamma-ray variability: (1) rather constant baseline with sporadic flaring activity characterized by flatter PDS slopes resembling flickering and red noise with occasional intermittence and (2)—measured for a few blazars showing strong activity—complex and structured temporal profiles characterized by long-term memory and steeper PDS slopes, reflecting a random walk underlying mechanism. The average slope of the PDS of the brightest 22 FSRQs and of the 6 brightest BL Lacs is 1.5 and 1.7, respectively. The study of temporal profiles of well-resolved flares observed in the 10 brightest LBAS sources shows that they generally have symmetric profiles and that their total duration vary between 10 and 100 days. Results presented here can assist in source class recognition for unidentified sources and can serve as reference for more detailed analysis of the brightest gamma-ray blazars.« less

Kiloparsec Jet Properties of Hybrid, Low-, and High-Synchrotron-Peaked Blazars

NASA Astrophysics Data System (ADS)

Stanley, Ethan C.

Blazars are a rare class of active galactic nucleus (AGN) with relativistic jets closely aligned with the line of sight. Many aspects of the environments and kiloparsec-scale jet structure are not fully understood. Hybrid and high synchrotron peaked (HSP) blazars are two types of blazar that provide unique opportunities to study these jets. Hybrid blazars appear to have jets of differing morphology on each side of their core, suggesting that external factors shape their jet morphology. Three hybrid sources were investigated in radio, optical, and X-ray wavelengths: 8C 1849+670, PKS 2216-038, and PKS 1045-188. For all three, X-ray emission was detected only from the approaching jet. All three had jet radio flux densities and emission mechanisms similar to higher-power FR II sources, but two had approaching jets similar to lower-power FR I sources. None of the three showed definitive signs of asymmetry in their external environments. These results agree with previous multiwavelength studies of hybrid sources that show a dominance of FR I approaching jets and FR II emission mechanisms. With the addition of these three hybrid sources, 13 have been studied in total. Eleven have FR I approaching jets, and eight of those have FR II emission mechanisms. These trends may be due to small number statistics, or they may indicate other factors are creating hybrid-like appearances. High synchrotron peaked blazars are defined by the frequency of the peak of their jet synchrotron emission. Some have shown extreme variability which would imply incredibly-powerful and well-aligned jets, but VLBA observations have measured only modest jet speeds. A radio survey was performed to measure the extended radio luminosity of a large sample of HSP sources. These sources were compared to the complete radio flux density limited MOJAVE 1.5 Jy sample. Flat spectrum radio quasars (FSRQs) showed significant overlap with low synchrotron peaked (LSP) BL Lacs in multiple parameters, which may suggest that many FSRQs are "masquerading'' as LSP BL Lacs. HSP BL Lacs showed slightly lower extended radio luminosities and significantly lower maximum apparent jet speeds, suggesting that they are intrinsically weaker sources. There was a good correlation between maximum apparent jet speed and extended radio luminosity, which supports using the extended radio luminosity as a measure of intrinsic jet power. There was a lack of TeV-detected sources with higher extended radio luminosities, which suggests TeV emission may favor low power jets or high synchrotron peak frequencies. The apparent low power of HSP sources and TeV-detected sources questions any model of TeV emission and variability that depends on the jet (or a part of it) being intrinsically powerful.

The X-ray view of radio-loud active galactic nuclei: The central engine and its environment

NASA Astrophysics Data System (ADS)

Donato, Davide

The non-thermal emission from many Active Galactic Nuclei (AGN) is obscured by optically thick circumnuclear matter, particularly at optical and ultraviolet wavelengths. In radio-loud (RL) sources, the AGN activity is coupled with the presence of a bipolar jet that emit radio through g-ray light which is relativistically beamed along the jet axes. The combination of absorption and beaming produces highly anisotropic radiation. The understanding of the origin and magnitude of this radiation allows astronomers to unify different classes of AGN; that is, to identify each single, underlying AGN type that gives rise to different classes through different orientations with respect to the jet axis. This is the fundamental notion behind what are called "unification models" of AGN. Although this general idea is well accepted, many aspects remain matter of debate. In fact, the explanation of the wide and complex variety of AGN phenomena must be searched in a combination of apparent differences (like orientation) and real differences in a number of physical parameters (like gas/dust content and distribution, luminosity, etc.). The goal of this thesis is to address some of the RL unification open questions using X-ray data. The improved sensitivity and angular resolution of a new generation of satellites, combined with the fact that X-rays provide useful information on a variety of AGN phenomena, will allow me to: (1) Study the broadband X-ray continua of BL Lacertae objects (BL Lacs) and Flat Spectrum Radio Quasars (FSRQs); (2) Probe the emission from the very inner region of an AGN; (3) Determine the presence and characteristic of extended X-ray emission from the AGN environment. The results obtained from theses studies will provide me insights into (1) the X-ray average spectral properties of BL Lacs and FSRQs and the physical processes responsible of the emission; (2) the presence of the obscuring torus and the amount of absorption, (3) the nature of X-ray emission, and (4) the type of accretion and efficiency of the accretion process in Fanaroff-Riley I radio galaxies; (5) the properties of the gas in BL Lacs and (6) its effects on the AGN activity and jet properties.

Optical Spectroscopic Observations of γ-Ray Blazar Candidates. III. The 2013/2014 Campaign in the Southern Hemisphere

NASA Astrophysics Data System (ADS)

Landoni, M.; Massaro, F.; Paggi, A.; D'Abrusco, R.; Milisavljevic, D.; Masetti, N.; Smith, H. A.; Tosti, G.; Chomiuk, L.; Strader, J.; Cheung, C. C.

2015-05-01

We report the results of our exploratory program carried out with the southern Astrophysical Research telescope aimed at associating counterparts and establishing the nature of the Fermi Unidentified γ-ray Sources (UGSs). We selected the optical counterparts of six UGSs from the Fermi catalog on the basis of our recently discovered tight connection between infrared and γ-ray emission found for the γ-ray blazars detected by the Wide-Field Infrared Survey Explorer in its all-sky survey. We perform for the first time a spectroscopic study of the low-energy counterparts of the Fermi UGSs, in the optical band, confirming the blazar-like nature of the whole sample. We also present new spectroscopic observations of six active galaxies of uncertain type associated with Fermi sources which appear to be BL Lac objects. Finally, we report the spectra collected for six known γ-ray blazars belonging to the Roma BZCAT that were obtained to establish their nature or better estimate their redshifts. Two interesting cases of high redshift and extremely luminous BL Lac objects (z ≥ 1.18 and z ≥ 1.02, based on the detection of Mg ii intervening systems) are also discussed. Based on observations obtained at the southern Astrophysical Research (SOAR) telescope, which is a joint project of the Ministério da Ciência, Tecnologia, e Inovação (MCTI) da República Federativa do Brasil, the U.S. National Optical Astronomy Observatory (NOAO), the University of North Carolina at Chapel Hill (UNC), and Michigan State University (MSU).

Detection of the BL Lac object 1ES 1426+428 in the Very High Energy gamma-ray band by the CAT Telescope from 1998-2000

NASA Astrophysics Data System (ADS)

Djannati-Ataï, A.; Khelifi, B.; Vorobiov, S.; Bazer-Bachi, R.; Chounet, L. M.; Debiais, G.; Degrange, B.; Espigat, P.; Fabre, B.; Fontaine, G.; Goret, P.; Gouiffes, C.; Masterson, C.; Piron, F.; Punch, M.; Rivoal, M.; Rob, L.; Tavernet, J.-P.

2002-08-01

The BL Lac Object 1ES 1426+428, at a red-shift of z=0.129, has been monitored by the CAT telescope from February 1998 to June 2000. The accumulation of 26 h of observations shows a gamma -ray signal of 321 events above 250 GeV at 5.2 standard deviations, determined using data analysis cuts adapted to a weak, steep-spectrum source. The source emission has an average flux of Phidiff(400 GeV)= 6.73+/-1.27stat+/-1.45syst*E-11 cm-2 s-1 TeV-1, and a very steep spectrum, with a differential spectral index of gamma =-3.60 +/- 0.57 which can be refined to gamma =-3.66 +/- 0.41 using a higher flux data subset. If, as expected from its broad-band properties, the Very High Energy emission is hard at the source, these observations support a strong absorption effect of gamma-rays by the Intergalactic Infrared field.

Contemporaneous broadband observations of three high-redshift BL Lac objects

DOE PAGES

Ackerman, M.

2016-03-20

We have collected broadband spectral energy distributions (SEDs) of three BL Lac objects, 3FGL J0022.1-1855 (z=0.689), 3FGL J0630.9-2406 (z > ~1.239), and 3FGL J0811.2-7529 (z=0.774), detected by Fermi with relatively flat GeV spectra. By observing simultaneously in the near-IR to hard X-ray band, we can well characterize the high end of the synchrotron component of the SED. Thus, fitting the SEDs to synchro-Compton models of the dominant emission from the relativistic jet, we can constrain the underlying particle properties and predict the shape of the GeV Compton component. Standard extragalactic background light (EBL) models explain the high-energy absorption well, withmore » poorer fits for high UV models. The fits show clear evidence for EBL absorption in the Fermi spectrum of our highest redshift source 3FGL J0630.9-2406. While synchrotron self-Compton models adequately describe the SEDs, the situation may be complicated by possible external Compton components.« less

Probing the EBL Evolution at High Redshift Using GRBs Detected with the Fermi-LAT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Desai, A.; Ajello, M.; Omodei, N.

The extragalactic background light (EBL), from ultraviolet to infrared wavelengths, is predominantly due to emission from stars, accreting black holes and reprocessed light due to Galactic dust. The EBL can be studied through the imprint it leaves, via γ–γ absorption of high-energy photons, in the spectra of distant γ-ray sources. The EBL has been probed through the search for the attenuation it produces in the spectra of BL Lacertae (BL Lac) objects and individual γ-ray bursts (GRBs). GRBs have significant advantages over blazars for the study of the EBL especially at high redshifts. Here we analyze a combined sample ofmore » 22 GRBs, detected by the Fermi Large Area Telescope between 65 MeV and 500 GeV. We report a marginal detection (at the ~2.8σ level) of the EBL attenuation in the stacked spectra of the source sample. This measurement represents a first constraint of the EBL at an effective redshift of ~1.8. Here, we combine our results with prior EBL constraints and conclude that Fermi-LAT is instrumental to constrain the UV component of the EBL. We discuss the implications on existing empirical models of EBL evolution.« less

Probing the EBL Evolution at High Redshift Using GRBs Detected with the Fermi-LAT

DOE PAGES

Desai, A.; Ajello, M.; Omodei, N.; ...

2017-11-17

The extragalactic background light (EBL), from ultraviolet to infrared wavelengths, is predominantly due to emission from stars, accreting black holes and reprocessed light due to Galactic dust. The EBL can be studied through the imprint it leaves, via γ–γ absorption of high-energy photons, in the spectra of distant γ-ray sources. The EBL has been probed through the search for the attenuation it produces in the spectra of BL Lacertae (BL Lac) objects and individual γ-ray bursts (GRBs). GRBs have significant advantages over blazars for the study of the EBL especially at high redshifts. Here we analyze a combined sample ofmore » 22 GRBs, detected by the Fermi Large Area Telescope between 65 MeV and 500 GeV. We report a marginal detection (at the ~2.8σ level) of the EBL attenuation in the stacked spectra of the source sample. This measurement represents a first constraint of the EBL at an effective redshift of ~1.8. Here, we combine our results with prior EBL constraints and conclude that Fermi-LAT is instrumental to constrain the UV component of the EBL. We discuss the implications on existing empirical models of EBL evolution.« less

Multiwavelength observations of the blazar 1ES 1011+496 in Spring 2008

NASA Astrophysics Data System (ADS)

Ahnen, M. L.; Ansoldi, S.; Antonelli, L. A.; Antoranz, P.; Babic, A.; Banerjee, B.; Bangale, P.; Barres de Almeida, U.; Barrio, J. A.; Becerra González, J.; Bednarek, W.; Bernardini, E.; Biasuzzi, B.; Biland, A.; Blanch, O.; Bonnefoy, S.; Bonnoli, G.; Borracci, F.; Bretz, T.; Carmona, E.; Carosi, A.; Chatterjee, A.; Clavero, R.; Colin, P.; Colombo, E.; Contreras, J. L.; Cortina, J.; Covino, S.; Da Vela, P.; Dazzi, F.; De Angelis, A.; De Caneva, G.; De Lotto, B.; de Oña Wilhelmi, E.; Delgado Mendez, C.; Di Pierro, F.; Dominis Prester, D.; Dorner, D.; Doro, M.; Einecke, S.; Elsaesser, D.; Fernández-Barral, A.; Fidalgo, D.; Fonseca, M. V.; Font, L.; Frantzen, K.; Fruck, C.; Galindo, D.; García López, R. J.; Garczarczyk, M.; Garrido Terrats, D.; Gaug, M.; Giammaria, P.; Eisenacher Glawion, D.; Godinović, N.; González Muñoz, A.; Guberman, D.; Hanabata, Y.; Hayashida, M.; Herrera, J.; Hose, J.; Hrupec, D.; Hughes, G.; Idec, W.; Kodani, K.; Konno, Y.; Kubo, H.; Kushida, J.; La Barbera, A.; Lelas, D.; Lindfors, E.; Lombardi, S.; Longo, F.; López, M.; López-Coto, R.; López-Oramas, A.; Lorenz, E.; Majumdar, P.; Makariev, M.; Mallot, K.; Maneva, G.; Manganaro, M.; Mannheim, K.; Maraschi, L.; Marcote, B.; Mariotti, M.; Martínez, M.; Mazin, D.; Menzel, U.; Miranda, J. M.; Mirzoyan, R.; Moralejo, A.; Nakajima, D.; Neustroev, V.; Niedzwiecki, A.; Nievas Rosillo, M.; Nilsson, K.; Nishijima, K.; Noda, K.; Orito, R.; Overkemping, A.; Paiano, S.; Palacio, J.; Palatiello, M.; Paneque, D.; Paoletti, R.; Paredes, J. M.; Paredes-Fortuny, X.; Persic, M.; Poutanen, J.; Prada Moroni, P. G.; Prandini, E.; Puljak, I.; Reinthal, R.; Rhode, W.; Ribó, M.; Rico, J.; Rodriguez Garcia, J.; Rügamer, S.; Saito, T.; Satalecka, K.; Scapin, V.; Schultz, C.; Schweizer, T.; Shore, S. N.; Sillanpää, A.; Sitarek, J.; Snidaric, I.; Sobczynska, D.; Stamerra, A.; Steinbring, T.; Strzys, M.; Takalo, L.; Takami, H.; Tavecchio, F.; Temnikov, P.; Terzić, T.; Tescaro, D.; Teshima, M.; Thaele, J.; Torres, D. F.; Toyama, T.; Treves, A.; Verguilov, V.; Vovk, I.; Ward, J. E.; Will, M.; Wu, M. H.; Zanin, R.; Lucarelli, F.; Pittori, C.; Vercellone, S.; Berdyugin, A.; Carini, M. T.; Lähteenmäki, A.; Pasanen, M.; Pease, A.; Sainio, J.; Tornikoski, M.; Walters, R.

2016-07-01

The BL Lac object 1ES 1011+496 was discovered at very high energy (VHE, E > 100GeV) γ-rays by Major Atmospheric Gamma-ray Imaging Cherenkov (MAGIC) in Spring 2007. Before that the source was little studied in different wavelengths. Therefore, a multiwavelength (MWL) campaign was organized in Spring 2008. Along MAGIC, the MWL campaign included the Metsähovi Radio Observatory, Bell and Kungliga Vetenskapsakademien (KVA) optical telescopes and the Swift and AGILE satellites. MAGIC observations span from 2008 March to May for a total of 27.9 h, of which 19.4 h remained after quality cuts. The light curve showed no significant variability yielding an integral flux above 200 GeV of (1.3 ± 0.3) × 10-11 photons cm-2 s-1. The differential VHE spectrum could be described with a power-law function with a spectral index of 3.3 ± 0.4. Both results were similar to those obtained during the discovery. Swift X-ray Telescope observations revealed an X-ray flare, characterized by a harder-when-brighter trend, as is typical for high synchrotron peak BL Lac objects (HBL). Strong optical variability was found during the campaign, but no conclusion on the connection between the optical and VHE γ-ray bands could be drawn. The contemporaneous spectral energy distribution shows a synchrotron-dominated source, unlike concluded in previous work based on non-simultaneous data, and is well described by a standard one-zone synchrotron self-Compton model. We also performed a study on the source classification. While the optical and X-ray data taken during our campaign show typical characteristics of an HBL, we suggest, based on archival data, that 1ES 1011+496 is actually a borderline case between intermediate and high synchrotron peak frequency BL Lac objects.

VLA observations of a complete sample of extragalactic X-ray sources. II

NASA Technical Reports Server (NTRS)

Schild, R.; Zamorani, G.; Gioia, I. M.; Feigelson, E. D.; Maccacaro, T.

1983-01-01

A complete sample of 35 X-ray selected sources found with the Einstein Observatory has been observed with the Very Large Array at 6 cm to investigate the relationship between radio and X-ray emission in extragalactic objects. Detections include three active galactic nuclei (AGNs), two clusters or groups of galaxies, two individual galaxies, and two BL Lac objects. The frequency of radio emission in X-ray selected AGNs is compared with that of optically selected quasars using the integral radio-optical luminosity function. The result suggests that the probability for X-ray selected quasars to be radio sources is higher than for those optically selected. No obvious correlation is found in the sample between the richness of X-ray luminosity of the cluster and the presence of a galaxy with radio luminosity at 5 GHz larger than 10 to the 30th ergs/s/Hz.

Distributions of Gamma-Ray Bursts and Blazars in the L p-E p-Plane and Possible Implications for their Radiation Physics

NASA Astrophysics Data System (ADS)

Lyu, Fen; Liang, En-Wei; Liang, Yun-Feng; Wu, Xue-Feng; Zhang, Jin; Sun, Xiao-Na; Lu, Rui-Jing; Zhang, Bing

2014-09-01

We present a spectral analysis for a sample of redshift-known gamma-ray bursts (GRBs) observed with Fermi/GBM. Together with the results derived from our systematical spectral energy distribution modeling with the leptonic models for a Fermi/LAT blazar sample, we compare the distributions of the GRBs and the blazars by plotting the synchrotron peak luminosity (L s) and the corresponding peak photon energy E s of blazars in the L p-E p-plane of GRBs, where L p and E p are the peak luminosity and peak photon energy of the GRB time-integrated νf ν spectrum, respectively. The GRBs are in the high-L p, high-E p corner of the plane and a tight L p-E p relation is found, i.e., L_p\\propto E_p2.13^{+0.54-0.46}. Both flat spectrum radio quasars (FSRQs) and low-synchrotron peaking BL Lac objects (LBLs) are clustered in the low-E p, low-L p corner. Intermediate- and high-synchrotron peaking BL Lac objects (IBLs and HBLs) have E s ~ 2 × 10-3-102 keV and L s ~ 1044-1047 erg s-1, but no dependence of L s on E s is found. We show that the tight Lp -Ep relation of GRBs is potentially explained with the synchrotron radiation of fast-cooling electrons in a highly magnetized ejecta, and the weak anti-correlation of L s-E s for FSRQs and LBLs may be attributed to synchrotron radiation of slow-cooling electrons in a moderately magnetized ejecta. The distributions of IBLs and HBLs in the L p-E p-plane may be interpreted with synchrotron radiation of fast-cooling electrons in a matter-dominated ejecta. These results may present a unified picture for the radiation physics of relativistic jets in GRBs and blazars within the framework of the leptonic synchrotron radiation models.

Radio variability in complete samples of extragalactic radio sources at 1.4 GHz

NASA Astrophysics Data System (ADS)

Rys, S.; Machalski, J.

1990-09-01

Complete samples of extragalactic radio sources obtained in 1970-1975 and the sky survey of Condon and Broderick (1983) were used to select sources variable at 1.4 GHz, and to investigate the characteristics of variability in the whole population of sources at this frequency. The radio structures, radio spectral types, and optical identifications of the selected variables are discussed. Only compact flat-spectrum sources vary at 1.4 GHz, and all but four are identified with QSOs, BL Lacs, or other (unconfirmed spectroscopically) stellar objects. No correlation of degree of variability at 1.4 GHz with Galactic latitude or variability at 408 MHz has been found, suggesting that most of the 1.4-GHz variability is intrinsic and not caused by refractive scintillations. Numerical models of the variability have been computed.

Fermi LAT detection of increased gamma-ray activity from blazar S5 0716+71

NASA Astrophysics Data System (ADS)

Buson, S.

2014-04-01

The Large Area Telescope (LAT), one of two instruments on-board the Fermi Gamma-ray Space Telescope, has observed an increase in gamma-ray activity from a source positionally coincident with the BL Lac object S5 0716+71 (also known as 2FGL J0721.9+7120, Nolan et al. ...

The first Fermi multifrequency campaign on BL Lacertae: Characterizing the low-activity state of the eponymous blazar

DOE PAGES

Abdo, A. A.; Ackermann, M.; Ajello, M.; ...

2011-03-10

Here, we report on observations of BL Lacertae during the first 18 months of Fermi LAT science operations and present results from a 48 day multifrequency coordinated campaign from 2008 August 19 to 2008 October 7. The radio to gamma-ray behavior of BL Lac is unveiled during a low-activity state thanks to the coordinated observations of radio-band (Metsähovi and VLBA), near-IR/optical (Tuorla, Steward, OAGH, and MDM), and X-ray ( RXTE and Swift) observatories. No variability was resolved in gamma rays during the campaign, and the brightness level was 15 times lower than the level of the 1997 EGRET outburst. Moderatemore » and uncorrelated variability has been detected in UV and X-rays. The X-ray spectrum is found to be concave, indicating the transition region between the low- and high-energy components of the spectral energy distribution (SED). VLBA observation detected a synchrotron spectrum self-absorption turnover in the innermost part of the radio jet appearing to be elongated and inhomogeneous, and constrained the average magnetic field there to be less than 3 G. Over the following months, BL Lac appeared variable in gamma rays, showing flares (in 2009 April and 2010 January). There is no evidence for the correlation of gamma rays with the optical flux monitored from the ground in 18 months. The SED may be described by a single-zone or a two-zone synchrotron self-Compton (SSC) model, but a hybrid SSC plus external radiation Compton model seems to be preferred based on the observed variability and the fact that it provides a fit closest to equipartition.« less

Investigating Self-Perceptions and Resilience in Looked after Children

ERIC Educational Resources Information Center

Honey, Kyla L.; Rees, Paul; Griffey, Simon

2011-01-01

The perceptions of Looked After Children (LAC; n = 51), their Designated Teachers (DTs), and a sample of non-LAC (n = 99) were elicited. LAC held more positive self-perceptions than the non-LAC, and similarly positive ratings were given for the LAC by their DTs; but LAC held lower career aspirations than the non-LAC. LAC differed in their levels…

Detection of Possible Quasi-periodic Oscillations in the Long-term Optical Light Curve of the BL Lac Object OJ 287

NASA Astrophysics Data System (ADS)

Bhatta, G.; Zola, S.; Stawarz, Ł.; Ostrowski, M.; Winiarski, M.; Ogłoza, W.; Dróżdż, M.; Siwak, M.; Liakos, A.; Kozieł-Wierzbowska, D.; Gazeas, K.; Debski, B.; Kundera, T.; Stachowski, G.; Paliya, V. S.

2016-11-01

The detection of periodicity in the broadband non-thermal emission of blazars has so far been proven to be elusive. However, there are a number of scenarios that could lead to quasi-periodic variations in blazar light curves. For example, an orbital or thermal/viscous period of accreting matter around central supermassive black holes could, in principle, be imprinted in the multi-wavelength emission of small-scale blazar jets, carrying such crucial information about plasma conditions within the jet launching regions. In this paper, we present the results of our time series analysis of the ˜9.2 yr long, and exceptionally well-sampled, optical light curve of the BL Lac object OJ 287. The study primarily used the data from our own observations performed at the Mt. Suhora and Kraków Observatories in Poland, and at the Athens Observatory in Greece. Additionally, SMARTS observations were used to fill some of the gaps in the data. The Lomb-Scargle periodogram and the weighted wavelet Z-transform methods were employed to search for possible quasi-periodic oscillations in the resulting optical light curve of the source. Both methods consistently yielded a possible quasi-periodic signal around the periods of ˜400 and ˜800 days, the former with a significance (over the underlying colored noise) of ≥slant 99 % . A number of likely explanations for this are discussed, with preference given to a modulation of the jet production efficiency by highly magnetized accretion disks. This supports previous findings and the interpretation reported recently in the literature for OJ 287 and other blazar sources.

On the Redshift of TeV BL Lac Objects

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paiano, Simona; Falomo, Renato; Landoni, Marco

2017-03-10

We report results of a spectroscopic campaign carried out at the 10 m Gran Telescopio Canarias for a sample of 22 BL Lac objects detected (or candidates) at TeV energies, aiming to determine or constrain their redshift. This is of fundamental importance for the interpretation of their emission models and for population studies and is also mandatory for studying the interaction of high-energy photons with the extragalactic background light using TeV sources. Optical spectra with high signal-to-noise ratios in the range 4250–10000 Å were obtained to search for faint emission or absorption lines from both the host galaxy and themore » nucleus. We determine a new redshift for PKS 1424+240 ( z = 0.604) and a tentative one for 1ES 0033+595 ( z = 0.467). We are able to set new spectroscopic redshift lower limits for three other sources on the basis of Mg ii and Ca ii intervening absorption features: BZB J1243+3627 ( z > 0.483), BZB J1540+8155 ( z > 0.672), and BZB 0J2323+4210 ( z > 0.267). We confirm previous redshift estimates for four blazars: S3 0218+357 ( z = 0.944), 1ES 1215+303 ( z = 0.129), W Comae ( z = 0.102), and MS 1221.8+2452 ( z = 0.218). For the remaining targets, in seven cases (S2 0109+22, 3C 66A, VER J0521+211, S4 0954+65, BZB J1120+4214, S3 1227+25, BZB J2323+4210), we do not validate the proposed redshift. Finally, for all sources of still-unknown redshift, we set a lower limit based on the minimum equivalent width of absorption features expected from the host galaxy.« less

Generation of complete source samples from the Slew Survey

NASA Technical Reports Server (NTRS)

Schachter, Jonathan

1992-01-01

The Einstein Slew Survey consists of 819 bright X-ray sources, of which 636 (or 78 percent) are identified with counterparts in standard catalogs. We argue for the importance of bright X-ray surveys, and compare the slew results to the ROSAT all-sky survey. Also, we discuss statistical techniques for minimizing confusion in arcminute error circles in digitized data. We describe the 238 Slew Survey AGN, clusters, and BL Lac objects identified to date and their implications for logN-logS and source evolution studies. Also given is a catalog of 1075 sources detected in the Einstein Imaging Proportional Counter (IPC) Slew Survey of the X-ray sky. Five hundred fifty-four of these sources were not previously known as X-ray sources.

Development of amnesia in different mouse strains.

PubMed

Sinovyev, D R; Dubrovina, N I; Kulikov, A V

2009-05-01

We studied passive avoidance retrieval after amnestic stimulation (arrest in unsafe section of the experimental setup) in C57Bl/6J, BALB/c, CBA/Lac, AKR/J, DBA/2J, C3H/HeJ, and ASC/Icg mice. We demonstrated resistance to amnestic stimulation in mice with high predisposition to freezing reaction (ASC/Icg) and memory deficit in other mouse strains.

Redshift determination of the BL Lac object 3C 66A by the detection of its host galaxy cluster at z = 0.340

NASA Astrophysics Data System (ADS)

Torres-Zafra, Juanita; Cellone, Sergio A.; Buzzoni, Alberto; Andruchow, Ileana; Portilla, José G.

2018-03-01

The BL Lac object 3C 66A is one of the most luminous extragalactic sources at TeV γ-rays (very high energy, i.e. E > 100 GeV). Since TeV γ-ray radiation is absorbed by the extragalactic background light (EBL), it is crucial to know the redshift of the source in order to reconstruct its original spectral energy distribution, as well as to constrain EBL models. However, the optical spectrum of this BL Lac is almost featureless, so a direct measurement of z is very difficult; in fact, the published redshift value for this source (z = 0.444) has been strongly questioned. Based on EBL absorption arguments, several constraints to its redshift, in the range 0.096 < z < 0.5, were proposed. Since these active galactic nuclei (AGNs) are hosted, typically, in early-type galaxies that are members of groups or clusters, we have analysed spectro-photometrically the environment of 3C 66A, with the goal of finding the galaxy group hosting this blazar. This study was made using optical images of a 5.5 × 5.5 arcmin2 field centred on the blazar, and spectra of 24 sources obtained with Gemini/GMOS-N multi-object spectroscopy. We found spectroscopic evidence of two galaxy groups along the blazar's line of sight: one at z ≃ 0.020 and the second one at z ≃ 0.340. The first one is consistent with a known foreground structure, while the second group presented here has six spectroscopically confirmed members. Their location along a red sequence in the colour-magnitude diagram allows us to identify 34 additional candidate members of the more distant group. The blazar's spectrum shows broad absorption features that we identify as arising in the intergalactic medium, thus allowing us to tentatively set a redshift lower limit at z_3C66A ≳ 0.33. As a consequence, we propose that 3C 66A is hosted in a galaxy that belongs to a cluster at z = 0.340.

An Easy Method to Eliminate the Effect of Lupus Anticoagulants in the Coagulation Factor Assay.

PubMed

Tang, Ning; Yin, Shiyu

2016-07-01

To build and evaluate intrinsic coagulation factor assays which can eliminate the effect of lupus anticoagulants (LAC). Commercial silica clotting time confirmatory (SCT-C) reagent containing sufficient synthetic phospholipid and routine activated partial thromboplastin time (APTT) reagent were each used for one-stage detection of FVIII, FIX, and FXI activities, in samples with or without LAC, and the results were compared. For samples without LAC, consistent results of FVIII, FIX, and FXI using both SCT-C reagent and APTT reagent were obtained. For samples with LAC, the assays with SCT-C reagent not only could eliminate the effect of strong lupus anticoagulants but also needed fewer dilutions than that with routine APTT reagent. The intrinsic factor detections by SCT-C reagent are credible and convenient to be used for samples with LAC.

The Millimeter-Radio Emission of BL Lacertae During Two γ-ray Outbursts

NASA Astrophysics Data System (ADS)

Kim, Dae-Won; Trippe, Sascha; Lee, Sang-Sung; Park, Jong-Ho; Kim, Jae-Young; Algaba, Juan-Carlos; Hodgson, Jeffrey A.; Kino, Motoki; Zhao, Guang-Yao; Wajima, Kiyoaki; Kang, Sincheol; Oh, Junghwan; Lee, Taeseok; Byun, Do-Young; Kim, Soon-Wook; Kim, Jeong-Sook

2017-12-01

We present a study of the inexplicit connection between radio jet activity and γ-ray emission of BL Lacertae (BL Lac; 2200+420). We analyze the long-term millimeter activity of BL Lac via interferometric observations with the Korean VLBI Network (KVN) obtained at 22, 43, 86, and 129 GHz simultaneously over three years (from January 2013 to March 2016); during this time, two γ-ray outbursts (in November 2013 and March 2015) can be seen in γ-ray light curves obtained from Fermi observations. The KVN radio core is optically thick at least up to 86 GHz; there is indication that it might be optically thin at higher frequencies. To first order, the radio light curves decay exponentially over the time span covered by our observations, with decay timescales of 411±85 days, 352±79 days, 310±57 days, and 283±55 days at 22, 43, 86, and 129 GHz, respectively. Assuming synchrotron cooling, a cooling time of around one year is consistent with magnetic field strengths B=2 μT and electron Lorentz factors γ=10,000. Taking into account that our formal measurement errors include intrinsic variability and thus over-estimate the statistical uncertainties, we find that the decay timescale τ scales with frequency ν like τ∝ν^{-0.2}. This relation is much shallower than the one expected from opacity effects (core shift), but in agreement with the (sub-)mm radio core being a standing recollimation shock. We do not find convincing radio flux counterparts to the γ-ray outbursts. The spectral evolution is consistent with the 'generalized shock model' of Valtaoja et al. (1992). A temporary increase in the core opacity and the emergence of a knot around the time of the second γ-ray event indicate that this γ-ray outburst might be an 'orphan' flare powered by the 'ring of fire' mechanism.

p53 deficiency alters the yield and spectrum of radiation-induced lacZ mutants in the brain of transgenic mice

NASA Technical Reports Server (NTRS)

Chang, P. Y.; Kanazawa, N.; Lutze-Mann, L.; Winegar, R. A.

2001-01-01

Exposure to heavy particle radiation in the galacto-cosmic environment poses a significant risk in space exploration and the evaluation of radiation-induced genetic damage in tissues, especially in the central nervous system, is an important consideration in long-term manned space missions. We used a plasmid-based transgenic mouse model system, with the pUR288 lacZ transgene integrated in the genome of every cell of C57Bl/6(lacZ) mice, to evaluate the genetic damage induced by iron particle radiation. In order to examine the importance of genetic background on the radiation sensitivity of individuals, we cross-bred p53 wild-type lacZ transgenic mice with p53 nullizygous mice, producing lacZ transgenic mice that were either hemizygous or nullizygous for the p53 tumor suppressor gene. Animals were exposed to an acute dose of 1 Gy of iron particles and the lacZ mutation frequency (MF) in the brain was measured at time intervals from 1 to 16 weeks post-irradiation. Our results suggest that iron particles induced an increase in lacZ MF (2.4-fold increase in p53+/+ mice, 1.3-fold increase in p53+/- mice and 2.1-fold increase in p53-/- mice) and that this induction is both temporally regulated and p53 genotype dependent. Characterization of mutants based on their restriction patterns showed that the majority of the mutants arising spontaneously are derived from point mutations or small deletions in all three genotypes. Radiation induced alterations in the spectrum of deletion mutants and reorganization of the genome, as evidenced by the selection of mutants containing mouse genomic DNA. These observations are unique in that mutations in brain tissue after particle radiation exposure have never before been reported owing to technical limitations in most other mutation assays.

[Action of red polarized light on the acupuncture point E-36 increases analgesic effect of corvitin].

PubMed

Tamarova, Z A; Lymans'kyĭ, Iu P; Kostiuk, O I; Mitruzaeva, V A; Lymans'ka, L I

2010-01-01

In experiments on mice of lines C57BL/6J and CBA/CaLac, the possibility of strengthening of analgesic effect of corvitin by the action of red polarized light (PL) on the acupoint (AP) E-36 was studied. The pain behavioral response (licking of the painful area) was caused by injection of 5% formalin in hind limb (0.25 microl subcutaneously). The duration of pain response was studied before and after systemic introduction of corvitin (10 mg/kg, intraperitoneal) or joint use of corvitin and red PL (10 minute session). It is established, that after application of red PL on the antinociceptive AP E-36 in all animals an authentic strengthening of antinociceptive effect of corvitin takes place. In C57BL/6J mice, application of corvitin alone weakened the pain response by 29.7% and during combined use of red PL and corvitin, it grew up to 53.1%. Mice of line CBA/CaLac were less sensitive both to corvitin, and PL. In this line, corvitin used alone reduced the duration of pain response by 14%, and by 32.4% during combined use with red PL. Non-traumatic, without side effects, the method of influence by low-intensive PL can be recommended to patients accepting corvitin for strengthening its efficiency.

Multi-wavelength study of flaring activity in BL Lac object S5 0716+714 during the 2015 outburst

DOE PAGES

Chandra, Sunil; Zhang, Haocheng; Kushwaha, Pankaj; ...

2015-08-17

We present a detailed investigation of the flaring activity observed from a BL Lac object, S5 0716+714 , during its brightest ever optical state in the second half of 2015 January. Observed almost simultaneously in the optical, X-rays, and γ-rays, a significant change in the degree of optical polarization (PD) and a swing in the position angle (PA) of polarization were recorded. A TeV (VHE) detection was also reported by the MAGIC consortium during this flaring episode. Two prominent sub-flares, peaking about five days apart, were seen in almost all of the energy bands. The multi-wavelength light curves, spectral energymore » distribution, and polarization are modeled using the time-dependent code developed by Zhang et al. This model assumes a straight jet threaded by large-scale helical magnetic fields taking into account the light travel time effects, incorporating synchrotron flux and polarization in 3D geometry. Furthermore, the rapid variation in PD and rotation in PA are most likely due to reconnections happening in the emission region in the jet, as suggested by the change in the ratio of toroidal to poloidal components of the magnetic field during the quiescent and flaring states.« less

Determination of the Point-Spread Function for the FERMI Large Area Telescope from On-Orbit Data and Limits on Pair Halos of Active Galactic Nuclei

DOE PAGES

Ackermann, M.; Ajello, M.; Allafort, A.; ...

2013-02-15

We present the Large Area Telescope (LAT) on the Fermi Gamma-ray Space Telescope is a pair-conversion telescope designed to detect photons with energies from ≈20 MeV to >300 GeV. The pre-launch response functions of the LAT were determined through extensive Monte Carlo simulations and beam tests. The point-spread function (PSF) characterizing the angular distribution of reconstructed photons as a function of energy and geometry in the detector is determined here from two years of on-orbit data by examining the distributions of γ rays from pulsars and active galactic nuclei (AGNs). Above 3 GeV, the PSF is found to be broadermore » than the pre-launch PSF. We checked for dependence of the PSF on the class of γ-ray source and observation epoch and found none. We also investigated several possible spatial models for pair-halo emission around BL Lac AGNs. Finally, we found no evidence for a component with spatial extension larger than the PSF and set upper limits on the amplitude of halo emission in stacked images of low- and high-redshift BL Lac AGNs and the TeV blazars 1ES0229+200 and 1ES0347–121.« less

Long Term Observations of B2 1215+30 with VERITAS

NASA Astrophysics Data System (ADS)

Aliu, E.; Archambault, S.; Arlen, T.; Aune, T.; Beilicke, M.; Benbow, W.; Bird, R.; Bouvier, A.; Buckley, J. H.; Bugaev, V.; Cesarini, A.; Ciupik, L.; Connolly, M. P.; Cui, W.; Dumm, J.; Errando, M.; Falcone, A.; Federici, S.; Feng, Q.; Finley, J. P.; Fortin, P.; Fortson, L.; Furniss, A.; Galante, N.; Gérard, L.; Gillanders, G. H.; Griffin, S.; Grube, J.; Gyuk, G.; Hanna, D.; Holder, J.; Hughes, G.; Humensky, T. B.; Kaaret, P.; Kertzman, M.; Khassen, Y.; Kieda, D.; Krawczynski, H.; Krennrich, F.; Lang, M. J.; Madhavan, A. S.; Maier, G.; Majumdar, P.; McArthur, S.; McCann, A.; Moriarty, P.; Mukherjee, R.; Nieto, D.; O'Faoláin de Bhróithe, A.; Ong, R. A.; Orr, M.; Otte, A. N.; Park, N.; Perkins, J. S.; Pohl, M.; Popkow, A.; Prokoph, H.; Quinn, J.; Ragan, K.; Reyes, L. C.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Saxon, D. B.; Sembroski, G. H.; Skole, C.; Smith, A. W.; Soares-Furtado, M.; Staszak, D.; Telezhinsky, I.; Tešić, G.; Theiling, M.; Varlotta, A.; Vassiliev, V. V.; Vincent, S.; Wakely, S. P.; Weekes, T. C.; Weinstein, A.; Welsing, R.; Williams, D. A.; Zitzer, B.; VERITAS Collaboration; Böttcher, M.; Fumagalli, M.; Jadhav, J.

2013-12-01

We report on VERITAS observations of the BL Lac object B2 1215+30 between 2008 and 2012. During this period, the source was detected at very high energies (VHEs; E > 100 GeV) by VERITAS with a significance of 8.9σ and showed clear variability on timescales larger than months. In 2011, the source was found to be in a relatively bright state and a power-law fit to the differential photon spectrum yields a spectral index of 3.6 ± 0.4stat ± 0.3syst with an integral flux above 200 GeV of (8.0 ± 0.9stat ± 3.2syst) × 10-12 cm-2 s-1. No short term variability could be detected during the bright state in 2011. Multi-wavelength data were obtained contemporaneously with the VERITAS observations in 2011 and cover optical (Super-LOTIS, MDM, Swift/UVOT), X-ray (Swift/XRT), and gamma-ray (Fermi-LAT) frequencies. These were used to construct the spectral energy distribution (SED) of B2 1215+30. A one-zone leptonic model is used to model the blazar emission and the results are compared to those of MAGIC from early 2011 and other VERITAS-detected blazars. The SED can be reproduced well with model parameters typical for VHE-detected BL Lac objects.

Comparison of Optical and Multi-Waveband Variations of Selected Gamma-ray Bright Blazars in 2012

NASA Astrophysics Data System (ADS)

Schultz, Benjamin; Jorstad, S. G.; Marscher, A. P.; Williamson, K. E.; Walker, G. E.

2013-01-01

We present multi-wavelength observations of several gamma-ray bright blazars. We combine optical data obtained with the 17-inch CCD telescope of Maria Mitchell Observatory with space- and ground-based observations carried out with a variety of instruments. These include a number of other optical telescopes, the Fermi Gamma-Ray Space Telescope at photon energies of 0.1-200 GeV, and the Swift satellite at 0.3-10 keV plus optical and UV wavelengths. Three of the observed blazars proved to be particularly active - BL Lac, Mrk501, and CTA-102. BL Lac was of special interest, displaying remarkable activity in multiple wavelengths during this observation period, including the optical, in which it underwent its largest observed flare in a number of years. In addition, CTA-102 has recently undergone an unprecedented multi-wavelength outburst. We cross-correlate the variations in the different wavebands in an effort to guide theoretical interpretations of the optical and high-energy emission from blazars. This project was supported by NSF/REU grant AST-0851892 and the Nantucket Maria Mitchell Association. The research at Boston University was supported in part by NSF grant AST-0907893 and by NASA through Fermi grant NNX11AQ03G.

Multi-wavelength study of flaring activity in BL Lac object S5 0716+714 during the 2015 outburst

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chandra, Sunil; Zhang, Haocheng; Kushwaha, Pankaj

We present a detailed investigation of the flaring activity observed from a BL Lac object, S5 0716+714 , during its brightest ever optical state in the second half of 2015 January. Observed almost simultaneously in the optical, X-rays, and γ-rays, a significant change in the degree of optical polarization (PD) and a swing in the position angle (PA) of polarization were recorded. A TeV (VHE) detection was also reported by the MAGIC consortium during this flaring episode. Two prominent sub-flares, peaking about five days apart, were seen in almost all of the energy bands. The multi-wavelength light curves, spectral energymore » distribution, and polarization are modeled using the time-dependent code developed by Zhang et al. This model assumes a straight jet threaded by large-scale helical magnetic fields taking into account the light travel time effects, incorporating synchrotron flux and polarization in 3D geometry. Furthermore, the rapid variation in PD and rotation in PA are most likely due to reconnections happening in the emission region in the jet, as suggested by the change in the ratio of toroidal to poloidal components of the magnetic field during the quiescent and flaring states.« less

MULTI-WAVELENGTH STUDY OF FLARING ACTIVITY IN BL Lac OBJECT S5 0716+714 DURING THE 2015 OUTBURST

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chandra, Sunil; Kushwaha, Pankaj; Singh, K. P.

We present a detailed investigation of the flaring activity observed from a BL Lac object, S5 0716+714 , during its brightest ever optical state in the second half of 2015 January. Observed almost simultaneously in the optical, X-rays, and γ-rays, a significant change in the degree of optical polarization (PD) and a swing in the position angle (PA) of polarization were recorded. A TeV (VHE) detection was also reported by the MAGIC consortium during this flaring episode. Two prominent sub-flares, peaking about five days apart, were seen in almost all of the energy bands. The multi-wavelength light curves, spectral energymore » distribution, and polarization are modeled using the time-dependent code developed by Zhang et al. This model assumes a straight jet threaded by large-scale helical magnetic fields taking into account the light travel time effects, incorporating synchrotron flux and polarization in 3D geometry. The rapid variation in PD and rotation in PA are most likely due to reconnections happening in the emission region in the jet, as suggested by the change in the ratio of toroidal to poloidal components of the magnetic field during the quiescent and flaring states.« less

Quasi-simultaneous observations of BL Lac object Mrk 501 in X-ray, UV, visible, IR, and radio frequencies

NASA Technical Reports Server (NTRS)

Kondo, Y.; Worrall, D. M.; Oke, J. B.; Yee, H. K. C.; Neugebauer, G.; Matthews, K.; Feldman, P. A.; Mushotzky, R. F.; Hackney, R. L.; Hackney, K. R. H.

1981-01-01

Observations in the X-ray, UV, visible, IR and radio regions of the BL Lac object Mrk 501 made over the course of two months are reported. The measurements were made with the A2 experiment on HEAO 1 (X-ray), the SWP and LWR cameras on IUE (UV), the 5-m Hale telescope (visible), the 2.5-m telescope at Mount Wilson (IR), the NRAO 92-m radio telescope at Green Bank (4750 MHz) and the 46-m radio telescope at the Algonquin Observatory (10275 and 10650 MHz). The quasi-simultaneously observed spectral slope is found to be positive and continuous from the X-ray to the UV, but to gradually flatten and possibly turn down from the mid-UV to the visible; the optical-radio emission cannot be accounted for by a single power law. The total spectrum is shown to be compatible with a synchrotron self-Compton emission mechanism, while the spectrum from the visible to the X-ray is consistent with synchrotron radiation or inverse-Compton scattering by a hot thermal electron cloud. The continuity of the spectrum from the UV to the X-ray is noted to imply a total luminosity greater than previous estimates by a factor of 3-4.

Differences in lupus anticoagulant final conclusion through clotting time or Rosner index for mixing test interpretation.

PubMed

Depreter, Barbara; Devreese, Katrien M J

2016-09-01

Lupus anticoagulant (LAC) testing includes a screening, mixing and confirmation step. Although recently published guidelines on LAC testing are a useful step towards standardization, a lack of consensus remains whether to express mixing tests in clotting time (CT) or index of circulating anticoagulant (ICA). The influence of anticoagulant therapy, e.g. vitamin K antagonists (VKA) or direct oral anticoagulants (DOAC) on both methods of interpretation remains to be investigated. The objective of this study was to contribute to a simplification and standardization of the LAC three-step interpretation on the level of the mixing test. Samples from 148 consecutive patients with LAC request and prolonged screening step, and 77 samples from patients non-suspicious for LAC treated with VKA (n=37) or DOAC (n=30) were retrospectively evaluated. An activated partial thromboplastin time (aPTT) and dilute Russell's viper venom time (dRVVT) were used for routine LAC testing. The supplemental anticoagulant samples were tested with dRVVT only. We focused on the interpretation differences for mixing tests expressed as CT or ICA and compared the final LAC conclusion within each distinct group of concordant and discordant mixing test results. Mixing test interpretation by CT resulted in 10 (dRVVT) and 16 (aPTT) more LAC positive patients compared to interpretation with ICA. Isolated prolonged dRVVT screen mix ICA results were exclusively observed in samples from VKA-treated patients without suspicion for LAC. We recommend using CT in respect to the 99th percentile cut-off for interpretation of mixing steps in order to reach the highest sensitivity and specificity in LAC detection.

Long term monitoring of Gamma-Ray emission from the BL Lacertae object (1ES 2200+420)

NASA Astrophysics Data System (ADS)

Gunawardhana, Isuru; VERITAS Collaboration

2016-03-01

Blazars are a class of Active Galactic Nuclei (AGN) that have relativistic jets pointing along the observer line of sight. Blazars exhibit variable emission extending from radio to TeV energies. The variability timescale of the TeV flux is a key component of understanding the location of the very high energy emission zones. Deep observations of the quiescent state measurements are also required to disentangle the flaring state emission from quiescent state emission, a prerequisite for understanding the origin of blazar spectral variability. BL Lacertae (also known as 1ES 2200+420), as the namesake for all BL Lac objects, is a prime example of one such blazar. The VERITAS Observatory, an Imaging Atmospheric Cherenkov Telescope (IACT) array sensitive to gamma rays in the range from 85 GeV to 30 TeV, dedicates approximately 110 hours per year on deep observations of known gamma-ray blazars. In this talk, I will describe the TeV photon flux variability of BL Lacertae measured by VERITAS from 2013 to 2015.

Pearson-Readhead Survey Sources. II. The Long-Term Centimeter-Band Total Flux and Linear Polarization Properties of a Complete Radio Sample

NASA Astrophysics Data System (ADS)

Aller, M. F.; Aller, H. D.; Hughes, P. A.

2003-03-01

Using UMRAO centimeter-band total flux density and linear polarization monitoring observations of the complete Pearson-Readhead extragalactic source sample obtained between 1984 August and 2001 March, we identify the range of variability in extragalactic objects as functions of optical and radio morphological classification and relate total flux density variations to structural changes in published coeval VLBI maps in selected objects. As expected, variability is common in flat- or inverted-spectrum (α<=0.5) core-dominated QSOs and BL Lac objects. Unexpectedly, we find flux variations in several steep-spectrum sample members, including the commonly adopted flux standard 3C 147. Such variations are characteristically several-year rises or declines or infrequent outbursts, requiring long-term observations for detection: we attribute them to the brightening of weak core components, a change that is suppressed by contributions from extended structure in all but the strongest events, and identify a wavelength dependence for the amplitude of this variability consistent with the presence of opacity in some portions of the jet flow. One morphological class of steep-spectrum objects, the compact symmetric objects (CSOs), characteristically shows only low-level variability. We examine the statistical relation between fractional polarization and radio class based on the data at 14.5 and 4.8 GHz. The blazars typically exhibit flat-to-inverted polarization spectra, a behavior attributed to opacity effects. Among the steep-spectrum objects, the lobe-dominated FR I galaxies have steep fractional polarization spectra, while the FR II galaxies exhibit fractional polarization spectra ranging from inverted to steep, with no identifiable common property that accounts for the range in behavior. For the CSO/gigahertz-peaked spectrum sources, we verify that the fractional polarizations at 4.8 GHz are only of the order of a few tenths of a percent, but at 14.5 GHz we find significantly higher polarizations, ranging from 1% to 3%; this frequency dependence supports a scenario invoking Faraday depolarization by a circumnuclear torus. We have identified preferred orientations of the electric vector of the polarized emission (EVPA) at 14.5 and 4.8 GHz in roughly half of the objects and compared these with orientations of the flow direction indicated by VLBI morphology. When comparing the distributions of the orientation offsets for the BL Lac objects and the QSOs, we find differences in both range and mean value, in support of intrinsic class differences. In the shock-in-jet scenario, we attribute this to the allowed range of obliquities of shocks developing in the flow relative to the flow direction: in the BL Lac objects the shocks are nearly transverse to the flow direction, while in the QSOs they include a broader range of obliquities and can be at large angles to it. The fact that we find long-term stability in EVPA over many events implies that a dominant magnetic field orientation persists; in the core-dominated objects, with small contribution from the underlying quiescent jet, this plausibly suggests that the magnetic field has a long-term memory, with subsequent shock events exhibiting similar EVPA orientation, or, alternatively, the presence of a standing shock in the core. We have looked for systematic, monotonic changes in EVPA, which might be expected in the emission from a precessing jet, a model currently invoked for some AGNs; none were identified. Further, we carried out a Scargle periodogram analysis of the total flux density observations, but found no strong evidence for periodicity in any of the sample sources. The only well-established case in support of both jet precession and periodic variability remains the non-sample member OJ 287.

A Mouse Model of Acinetobacter baumannii-Associated Pneumonia Using a Clinically Isolated Hypervirulent Strain

PubMed Central

Harris, Greg; Kuo Lee, Rhonda; Lam, Christopher K.; Kanzaki, Gregory; Patel, Girishchandra B.; Xu, H. Howard

2013-01-01

Acinetobacter baumannii is an important emerging pathogen in health care-acquired infections and is responsible for severe nosocomial and community-acquired pneumonia. Currently available mouse models of A. baumannii pneumonia show poor colonization with little to no extrapulmonary dissemination. Here, we describe a mouse model of A. baumannii pneumonia using a clinical isolate (LAC-4 strain) that reliably reproduces the most relevant features of human pulmonary A. baumannii infection and pathology. Using this model, we have shown that LAC-4 infection induced rapid bacterial replication in the lungs, significant extrapulmonary dissemination, and severe bacteremia by 24 h postintranasal inoculation. Infected mice showed severe bronchopneumonia and dilatation and inflammatory cell infiltration in the perivascular space. More significantly, 100% of C57BL/6 and BALB/c mice succumbed to 108 CFU of LAC-4 inoculation within 48 h. When this model was used to assess the efficacy of antimicrobials, all mice treated with imipenem and tigecycline survived a lethal intranasal challenge, with minimal clinical signs and body weight loss. Moreover, intranasal immunization of mice with formalin-fixed LAC-4 protected 40% of mice from a lethal (100× 100% lethal dose) intraperitoneal challenge. Thus, this model offers a reproducible acute course of A. baumannii pneumonia without requiring additional manipulation of host immune status, which will facilitate the development of therapeutic agents and vaccines against A. baumannii pneumonia in humans. PMID:23689726

A-3 scientific results - extragalactic

NASA Technical Reports Server (NTRS)

Schwartz, D. A.

1979-01-01

The results of the HEAO A-3 experiment are summarized. Specific contributions of the experiment to extragalactic astronomy are emphasized. The discovery of relatively condensed X-ray emission in the cores of those clusters of galaxies which are dominated by a giant elliptical or cD galaxy, the discovery of extended X-ray emitting plasma in groups of galaxies, and the demonstration that BL Lac objects are a class of X-ray sources are among the topics discussed.

Studies of the jet in BL Lacertae. I. Recollimation shock and moving emission features

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cohen, M. H.; Hovatta, T.; Meier, D. L.

2014-06-01

Parsec-scale VLBA images of BL Lac at 15 GHz show that the jet contains a permanent quasi-stationary emission feature 0.26 mas (0.34 pc projected) from the core, along with numerous moving features. In projection, the tracks of the moving features cluster around an axis at a position angle of –166.°6 that connects the core with the standing feature. The moving features appear to emanate from the standing feature in a manner strikingly similar to the results of numerical two-dimensional relativistic magneto-hydrodynamic (RMHD) simulations in which moving shocks are generated at a recollimation shock (RCS). Because of this, and the closemore » analogy to the jet feature HST-1 in M87, we identify the standing feature in BL Lac as an RCS. We assume that the magnetic field dominates the dynamics in the jet, and that the field is predominantly toroidal. From this we suggest that the moving features are compressions established by slow and fast mode magneto-acoustic MHD waves. We illustrate the situation with a simple model in which the slowest moving feature is a slow-mode wave, and the fastest feature is a fast-mode wave. In the model, the beam has Lorentz factor Γ{sub beam}{sup gal}≈3.5 in the frame of the host galaxy and the fast mode wave has Lorentz factor Γ{sub Fwave}{sup beam}≈1.6 in the frame of the beam. This gives a maximum apparent speed for the moving features, β{sub app} = v{sub app}/c = 10. In this model the Lorentz factor of the pattern in the galaxy frame is approximately three times larger than that of the beam itself.« less

Transmission of Atherosclerosis Susceptibility with Gut Microbial Transplantation*

PubMed Central

Gregory, Jill C.; Buffa, Jennifer A.; Org, Elin; Wang, Zeneng; Levison, Bruce S.; Zhu, Weifei; Wagner, Matthew A.; Bennett, Brian J.; Li, Lin; DiDonato, Joseph A.; Lusis, Aldons J.; Hazen, Stanley L.

2015-01-01

Recent studies indicate both clinical and mechanistic links between atherosclerotic heart disease and intestinal microbial metabolism of certain dietary nutrients producing trimethylamine N-oxide (TMAO). Here we test the hypothesis that gut microbial transplantation can transmit choline diet-induced TMAO production and atherosclerosis susceptibility. First, a strong association was noted between atherosclerotic plaque and plasma TMAO levels in a mouse diversity panel (n = 22 strains, r = 0.38; p = 0.0001). An atherosclerosis-prone and high TMAO-producing strain, C57BL/6J, and an atherosclerosis-resistant and low TMAO-producing strain, NZW/LacJ, were selected as donors for cecal microbial transplantation into apolipoprotein e null mice in which resident intestinal microbes were first suppressed with antibiotics. Trimethylamine (TMA) and TMAO levels were initially higher in recipients on choline diet that received cecal microbes from C57BL/6J inbred mice; however, durability of choline diet-dependent differences in TMA/TMAO levels was not maintained to the end of the study. Mice receiving C57BL/6J cecal microbes demonstrated choline diet-dependent enhancement in atherosclerotic plaque burden as compared with recipients of NZW/LacJ microbes. Microbial DNA analyses in feces and cecum revealed transplantation of donor microbial community features into recipients with differences in taxa proportions between donor strains that were transmissible to recipients and that tended to show coincident proportions with TMAO levels. Proportions of specific taxa were also identified that correlated with plasma TMAO levels in donors and recipients and with atherosclerotic lesion area in recipients. Atherosclerosis susceptibility may be transmitted via transplantation of gut microbiota. Gut microbes may thus represent a novel therapeutic target for modulating atherosclerosis susceptibility. PMID:25550161

ON ULTRA-HIGH-ENERGY COSMIC RAYS AND THEIR RESULTANT GAMMA-RAYS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gavish, Eyal; Eichler, David

2016-05-01

The Fermi Large Area Telescope collaboration has recently reported on 50 months of measurements of the isotropic extragalactic gamma-ray background (EGRB) spectrum between 100 MeV and 820 GeV. Ultra-high-energy cosmic ray (UHECR) protons interact with the cosmic microwave background photons and produce cascade photons of energies 10 MeV–1 TeV that contribute to the EGRB flux. We examine seven possible evolution models for UHECRs and find that UHECR sources that evolve as the star formation rate (SFR), medium low luminosity active galactic nuclei type-1 ( L = 10{sup 43.5} erg s{sup −1} in the [0.5–2] KeV band), and BL Lacertae objectsmore » (BL Lacs) are the most acceptable given the constraints imposed by the observed EGRB. Other possibilities produce too much secondary γ -radiation. In all cases, the decaying dark matter (DM) contribution improves the fit at high energy, but the contribution of still unresolved blazars, which would leave the smallest role for decaying DM, may yet provide an alternative improvement. The possibility that the entire EGRB can be fitted with resolvable but not-yet-resolved blazars, as recently claimed by Ajello et al., would leave little room in the EGRB to accommodate γ -rays from extragalactic UHECR production, even for many source evolution rates that would otherwise be acceptable. We find that under the assumption of UHECRs being mostly protons, there is not enough room for producing extragalactic UHECRs with active galactic nucleus, gamma-ray burst, or even SFR source evolution. Sources that evolve as BL Lacs, on the other hand, would produce much less secondary γ -radiation and would remain a viable source of UHECRs, provided that they dominate.« less

Multiwavelength and Statistical Research in Space Astrophysics

NASA Technical Reports Server (NTRS)

Feigelson, Eric D.

1997-01-01

The accomplishments in the following three research areas are summarized: multiwavelength study of active galactic nuclei; magnetic activity of young stellar objects; and statistical methodology for astronomical data analysis. The research is largely based on observations of the ROSAT and ASCA X-ray observatories, complemented by ground-based optical and radio studies. Major findings include: discovery of inverse Compton X-ray emission from radio galaxy lobes; creation of the largest and least biased available sample of BL Lac objects; characterization of X-ray and nonthermal radio emission from T Tauri stars; obtaining an improved census of young stars in a star forming region and modeling the star formation history and kinematics; discovery of X-ray emission from protostars; development of linear regression methods and codes for interpreting astronomical data; and organization of the first cross-disciplinary conferences for astronomers and statisticians.

Near-infrared and gamma-ray monitoring of TANAMI gamma-ray bright sources

DOE PAGES

Nesci, R.; Tosti, G.; Pursimo, T.; ...

2013-06-18

Context. We present that spectral energy distribution and its variability are basic tools for understanding the physical processes operating in active galactic nuclei (AGN). Aims. In this paper we report the results of a one-year near-infrared (NIR) and optical monitoring of a sample of 22 AGN known to be gamma-ray emitters, aimed at discovering correlations between optical and gamma-ray emission. Methods. We observed our objects with the Rapid Eye Mount (REM) telescope in J,H,K, and R bands nearly twice every month during their visibility window and derived light curves and spectral indexes. We also analyzed the gamma-ray data from themore » Fermi gamma-ray Space Telescope, making weekly averages. Results. Six sources were never detected during our monitoring, proving to be fainter than their historical Two micron all sky survey (2MASS) level. All of the sixteen detected sources showed marked flux density variability, while the spectral indexes remained unchanged within our sensitivity limits. Steeper sources showed, on average, a larger variability. From the NIR light curves we also computed a variability speed index for each detected source. Only one source (PKS 0208-512) underwent an NIR flare during our monitoring. Half of the sources showed a regular flux density trend on a one-year time scale, but do not show any other peculiar characteristic. The broadband spectral index α ro appears to be a good proxy of the NIR spectral index only for BL Lac objects. No clear correlation between NIR and gamma-ray data is evident in our data, save for PKS 0537-441, PKS 0521-360, PKS 2155-304, and PKS 1424-418. In conclusion, the gamma-ray/NIR flux ratio showed a large spread, QSO being generally gamma-louder than BL Lac, with a marked correlation with the estimated peak frequency (ν peak) of the synchrotron emission.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xia, Jun-Qing; Cuoco, Alessandro; Branchini, Enzo

Building on our previous cross-correlation analysis (Xia et al. 2011) between the isotropic γ-ray background (IGRB) and different tracers of the large-scale structure of the universe, we update our results using 60 months of data from the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope (Fermi). For this study, we perform a cross-correlation analysis both in configuration and spherical harmonics space between the IGRB and objects that may trace the astrophysical sources of the IGRB: QSOs in the Sloan Digital Sky Survey (SDSS) DR6, the SDSS DR8 Main Galaxy Sample, luminous red galaxies (LRGs) in the SDSS catalog, infrared-selected galaxies in the Two Micron All Sky Survey (2MASS), and radio galaxies in the NRAO VLA Sky Survey (NVSS). The benefit of correlating the Fermi-LAT signal with catalogs of objects at various redshifts is to provide tomographic information on the IGRB, which is crucial in separating the various contributions and clarifying its origin. The main result is that, unlike in our previous analysis, we now observe a significant (>3.5σ) cross-correlation signal on angular scales smaller than 1° in the NVSS, 2MASS, and QSO cases and, at lower statistical significance (~3.0σ), with SDSS galaxies. The signal is stronger in two energy bands, E > 0.5 GeV and E > 1 GeV, but it is also seen at E > 10 GeV. No cross-correlation signal is detected between Fermi data and the LRGs. These results are robust against the choice of the statistical estimator, estimate of errors, map cleaning procedure, and instrumental effects. Finally, we test the hypothesis that the IGRB observed by Fermi-LAT originates from the summed contributions of three types of unresolved extragalactic sources: BL Lacertae objects (BL Lacs), flat spectrum radio quasars (FSRQs), and star-forming galaxies (SFGs). Finally, we find that a model in which the IGRB is mainly produced by SFGs (more » $$72_{-37}^{+23}$$% with 2σ errors), with BL Lacs and FSRQs giving a minor contribution, provides a good fit to the data. We also consider a possible contribution from misaligned active galactic nuclei, and we find that, depending on the details of the model and its uncertainty, they can also provide a substantial contribution, partly degenerate with the SFG one.« less

A comparative study of thermochemical and cold plasma treatment on lignin-based activated carbon for adsorbing Fe(III)

NASA Astrophysics Data System (ADS)

Shi, Shukai; Wang, Xin; Chen, Weimin; Chen, Minzhi; Zhou, Xiaoyan

2018-05-01

The as-prepared lignin-based activated carbon (LAC) was post-treated by urea and radio-frequency cold plasma separately. The obtained results demonstrated that the BET surface and total volumes of the LAC and plasma-treated LACs were greater than the urea-modified sample. The analysis of surface elemental composition showed that the nitrogen content of urea-modified LAC and nitrogen plasma-treated LAC are 3.79% and 2.62% higher than that of original LAC respectively, while the oxygen content of air plasma-treated LAC is 10.23% higher than that of original LAC. The Fe(III) ions adsorbed studies with pseudo-second order kinetic model revealed that urea-modified LAC had faster chemisorption rates while air plasma-treated LAC had larger adsorption capacity within 3 h. Moreover, the adsorption capacity and chemisorption rates of LAC post-treated by nitrogen plasma are inferior to the air plasma-treated LAC.

Spectroscopic monitoring of the BL Lac object AO 0235+164

NASA Astrophysics Data System (ADS)

Raiteri, C. M.; Villata, M.; Capetti, A.; Heidt, J.; Arnaboldi, M.; Magazzù, A.

2007-03-01

Aims:Spectroscopic monitoring of BL Lac objects is a difficult task that nonetheless can provide important information on the different components of the active galactic nucleus. Methods: We performed optical spectroscopic monitoring of the BL Lac object AO 0235+164 (z=0.94) with the VLT and TNG telescopes from Aug. 2003 to Dec. 2004, during an extended WEBT campaign. The flux of this source is both contaminated and absorbed by a foreground galactic system at z=0.524, the stars of which can act as gravitational micro-lenses. Results: In this period the object was in an optically faint, though variable state, and a broad Mg II emission line was visible at all epochs. The spectroscopic analysis reveals an overall variation in the Mg II line flux of a factor 1.9, while the corresponding continuum flux density changed by a factor 4.3. Most likely, the photoionising radiation can be identified with the emission component that was earlier recognised to be present as a UV-soft-X-ray bump in the source spectral energy distribution and that is visible in the optical domain only in very faint optical states. We estimate an upper limit to the broad line region (BLR) size of a few light months from the historical minimum brightness level; from this we infer the maximum amplification of the Mg II line predicted by the microlensing scenario. Conclusions: .Unless we have strongly overestimated the size of the BLR, only very massive stars could significantly magnify the broad Mg II emission line, but the time scale of variations due to these (rare) events would be of several years. In contrast, the continuum flux, coming from much smaller emission regions in the jet, could be affected by microlensing from the more plausible MACHO deflectors, with variability time scales of the order of some months. Based on observations collected at the European Southern Observatory, Chile (ESO Programme 71.A-0174), and on observations made with the Italian Telescopio Nazionale Galileo (TNG) operated on the island of La Palma by the Fundación Galileo Galilei of the INAF (Istituto Nazionale di Astrofisica) at the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias.

First NuSTAR observations of the BL Lac - type blazar PKS 2155-304: constraints on the jet content and distribution of radiating particles

NASA Astrophysics Data System (ADS)

Madejski, Grzegorz; Nalewajko, Krzysztof; Madsen, Kristin; Chiang, James; Balokovic, Mislav; Paneque, David; Furniss, Amy; NuSTAR Team

2017-01-01

Current scenarios for emission mechanisms operating in relativistic jets in AGN involve synchrotron emission for the radio through UV spectrum, and inverse Compton for hard X-rays through γ-rays, but the particle content of relativistic jets - whether they are dominated by proton-electron, or e +/e- plasma - has not been established. Our first hard X-ray observations with NuSTAR of the BL Lac type blazar PKS 2155-304, augmented by XMM-Newton data, reveal the 0.5-60 keV spectrum as best-described by a soft power law component dominating below 10 keV (photon index of 3 at 2 keV), and a hard power-law tail (index 2), dominating in the 20-60 keV range. The hard X-ray tail can be smoothly joined to the quasi-simultaneous Fermi/LAT γ-ray spectrum by a synchrotron self-Compton component produced by an electron distribution with index p =2.2. The jet content needs to (globally) obey charge neutrality; assuming that the power-law electron distribution extends down to the Lorentz factor of 1, and one proton per electron, yields an unrealistically high total jet power of 1047 erg/s. This can be reduced by two orders of magnitude by considering a significant presence of e +/e- pairs with lepton-to-proton ratio of at least 30.

Comparison Of Optical, UV, X-ray, And Gamma-ray Variations Of Selected Blazars In 2011

NASA Astrophysics Data System (ADS)

Consiglio, Santina; Marscher, A. P.; Jorstad, S. G.; Walker, G.

2012-01-01

We present multi-wavelength observations of several gamma-ray bright blazars. We combine optical data obtained at Maria Mitchell Observatory on Nantucket Island with space- and ground-based observations carried out with a variety of instruments. These include a number of other optical telescopes, the Fermi Gamma-ray Space Telescope at photon energies of 0.1-200 GeV, the Rossi X-Ray Timing Explorer at 2.4-10 keV, and the Swift satellite at 0.3-10 keV plus optical and UV wavelengths. Three of the observed blazars proved to be particularly active - BL Lac, 3C 279, and PKS 1510-089. BL Lac was of special interest, varying greatly in optical brightness from night to night. In addition, as reported by the VERITAS group, it exhibited a remarkable, short-lived flare at TeV gamma-ray energies on one of the nights. We cross-correlate the variations in the different wavebands in an effort to guide theoretical interpretations of the optical and high-energy emission from blazars. This project was supported by NSF/REU grant AST-0851892 and by the Nantucket Maria Mitchell Association. The research at Boston University was supported in part by NSF grants AST-0907893, and by NASA through Fermi grants NNX08AV65G and NNX11AQ03G.

SIMULTANEOUS B'V'R' MONITORING OF BL LACERTAE OBJECT S5 0716+714 AND DETECTION OF INTER-BAND TIME DELAY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu Jianghua; He Xiangtao; Boettcher, Markus

We present the results of our optical monitoring of the BL Lac object S5 0716+714 over seven nights in 2006 December. The monitoring was carried out simultaneously at three optical wavelengths with a novel photometric system. The object did not show large-amplitude internight variations during this period. Intranight variations were observed on four nights and probably on one more. Strong bluer-when-brighter chromatism was detected on both intranight and internight timescales. The intranight variation amplitude decreases in the wavelength sequence of B', R', and V'. Cross-correlation analyses revealed that the variability at the B' and V' bands leads that at themore » R' band by about 30 minutes on one night.« less

A Search for Short Timescale Microvariability in Active Galactic Nuclei in the Ultraviolet

NASA Technical Reports Server (NTRS)

Dolan, Joseph F.; Clark, L. Lee

2003-01-01

We observed four AGNs (the type-1 Seyfert systems 3C249.1, NGC 6814 and Mrk 205, and the BL Lac object 3C371) using the High Speed Photometer on the Hubble Space Telescope to search for short timescale microvariability in the W. Continuous observations of 3 0 0 0 s duration were obtained for each system on several consecutive HST orbits using a 1 s sample time in a 1400 - 3000 2 bandpass. variability > 0.3 % (0 . 003 mag) was detected in any AGN on timescales shorter than 1500 s. The distribution of photon arrival times observed from each source was consistent with Poisson statistics. Because of HST optical problems, the limit on photometric variability at longer timescales is less precise. These results restrict models of supermassive black holes as the central engine of an AGN and the diskoseismology oscillations of any accretion disk around such a black hole.

Optical Spectroscopic Survey of a Sample of Unidentified Fermi Objects

NASA Astrophysics Data System (ADS)

Paiano, Simona; Falomo, Renato; Franceschini, Alberto; Treves, Aldo; Scarpa, Riccardo

2017-12-01

We present optical spectroscopy secured at the 10 m Gran Telescopio Canarias of the counterparts of 20 extragalactic γ-ray sources detected by the Fermi satellite. The observations allow us to investigate the nature of these sources and to determine their redshift. We find that all optical counterparts have a spectrum that is consistent with a BL Lac object nature. We are able to determine the redshift for 11 objects and set spectroscopic redshift limits for five targets. The optical spectrum is found featureless for only four sources. In the latter cases, we can set lower limits on the redshift based on the assumption that they are hosted by a typical massive elliptical galaxy whose spectrum is diluted by the nonthermal continuum. The observations allow us to unveil the nature of these gamma-ray sources and provide a sanity check of a tool to discover the counterparts of γ-ray emitters/blazars based on their multiwavelength emission.

VizieR Online Data Catalog: Blazars in the Swift-BAT hard X-ray sky (Maselli+, 2010)

NASA Astrophysics Data System (ADS)

Maselli, A.; Cusumano, G.; Massaro, E.; La Parola, V.; Segreto, A.; Sbarufatti, B.

2010-06-01

We reported the list of hard X-ray blazars obtained adopting sigma=3 as detection threshold: with this choice a number of three spurious sources over a total of 121 blazars is expected. Each blazar is identified by a three-letter code, where the first two are BZ for blazar and the third one specifies the type, followed by the truncated equatorial coordinates (J2000). The codes are defined in the "Note (1)" below. We obtained 69 FSRQs, 24 BL Lac objects and 28 blazars of uncertain classification, representing 4.4%, 2.4% and 11.0% of the corresponding populations classified in the BZCAT, respectively. This sample has been compared with other lists and catalogues found in literature (Tueller et al., 2010, Cat. J/ApJS/186/378, Ajello et al. 2009ApJ...699..603A, Cusumano et al., 2010, Cat. J/A+A/510/A48). (1 data file).

Milagro Observations of Potential TeV Emitters

NASA Astrophysics Data System (ADS)

Abeysekara, Anushka; Linnemann, James

2012-03-01

We searched for point sources in Milagro sky maps at the locations in four catalogs of potential TeV emitting sources. Our candidates are selected from the Fermi 2FGL pulsars, Fermi 2FGL extragalactic sources, TeVCat extragalactic sources, and from the BL Lac TeV Candidate list published by Costamante and Ghisellini in 2002. The False Discovery Rate (FDR) statistical procedure is used to select the sources. The FDR procedure controls the fraction of false detections. Our results are presented in this talk.

MAGIC discovers VHE gamma-ray emission from the blazar 1ES 1727+502

NASA Astrophysics Data System (ADS)

Mariotti, Mose

2011-11-01

The MAGIC Collaboration reports the discovery of Very High Energy (VHE; E>100 GeV) gamma-ray emission from the BL Lac object 1ES 1727+502 (also known as OT546) with redshift z=0.055. The source was selected from the compilation of Costamante, L. & Ghisellini, G. 2002, A&A, 384, 56. Previous observations with the single MAGIC-I telescope yielded an upper limit on the level of 11.8% of the Crab Nebula flux above 140 GeV (J.

GSFC Contributions to the NATO X-ray Astronomy Institute, Erice, July 1979. [X-ray spectra of supernova remants, galactic X-ray sources, active galactic nuclei, and clusters of galaxies

NASA Technical Reports Server (NTRS)

Holt, S. S.; Mushotzky, R. F.

1979-01-01

An overview of X-ray astronomical spectroscopy in general is presented and results obtained by HEAO 1 and 2 as well as earlier spacecraft are examined. Particular emphasis is given to the spectra of supernova remnants; galactic binary X-ray sources, cataclysmic variables, bulges, pulsars, and stars; the active nuclei of Seyfert 1 galaxy, BL Lac, and quasars; the diffuse X-ray background; and galactic clusters.

Extent of warm haloes around medium-redshift galaxies

NASA Technical Reports Server (NTRS)

Burbidge, E. M.; Barlow, T. A.; Cohen, R. D.; Junkkarinen, V. T.; Womble, D. S.

1989-01-01

The properties of low-to-medium ionization gaseous haloes around galaxies are briefly reviewed. New observations concerning such haloes are presented. For the galaxy-QSO pair in the field of the radio source 3C303, the higher-redshift QSO has been found to show Mg II absorption at the lower redshift of the faint nearby galaxy. Secondly, new data are presented on one of the galaxies in the environment of the well-known BL Lac object AO 0235 + 164.

VizieR Online Data Catalog: SDSS optically selected BL Lac candidates (Kuegler+, 2014)

NASA Astrophysics Data System (ADS)

Kuegler, S. D.; Nilsson, K.; Heidt, J.; Esser, J.; Schultz, T.

2014-11-01

The data that we use for variability and host galaxy analysis were presented in Paper I (Heidt & Nilsson, 2011A&A...529A.162H, Cat. J/A+A/529/A162). Alltogether, 123 targets were observed at the ESO New Technology Telescope (NTT) on La Silla, Chile during Oct. 2-6, 2008 and Mar. 28-Apr. 1, 2009. The observations were made with the EFOSC2 instrument through a Gunn-r filter (#786). (2 data files).

Characteristics of the LacTek test as applied to tissue samples: assessment of performance using incurred field samples.

PubMed

Mitchell, J M; McNab, W B; Yee, A J; Griffiths, M W; McEwen, S A; Spilsbury, L; Boison, J O

1998-08-01

The Lactek test, marketed for antimicrobial residue detection in milk, was validated for the detection of antimicrobial residues in tissues. A previous study found that the LacTek test could confidently identify tissue samples spiked with antimicrobial residues. However, the test could not reliably distinguish violative from nonviolative spiked samples relative to Canadian maximum residue limits (MRLs). The objectives of this study were to assess and compare the performance of the LacTek tests for beta-lactams, tetracyclines, gentamicin, and sulfamethazine on samples containing naturally incurred residues by running the test in parallel with the standard microbial inhibition test (MIT) presently used for the routine testing of tissues at our facility and to assess the agreement with high pressure liquid chromatographic (HPLC) determinative methods. Parallel testing with the official MIT found that the Lactek tests could be confidently used for testing tissue samples containing incurred residues. Among 1,008 MIT-positive samples, the LacTek test found that 90% contained beta-lactams and/or tetracyclines. A further 7.3% of violative residues could not be identified to an antimicrobial class. In addition, 9% of samples testing negative on the MIT were found to contain an antimicrobial residue by the LacTek tests. Comparative testing with HPLC methods found that there was very good agreement between the two tests and that most violations were due to penicillin G and oxytetracycline. Although the LacTek test cannot be used to distinguish violative from nonviolative residue levels, it does offer several advantages over the present MIT. These include speed, ease of use, the ability to identify residues to a specific class, and an improved sensitivity at the MRL level for the most commonly found antimicrobials in tissue.

Validation of the LacTek test applied to spiked extracts of tissue samples: determination of performance characteristics.

PubMed

Mitchell, J M; Yee, A J; McNab, W B; Griffiths, M W; McEwen, S A

1999-01-01

LacTek tests are competitive enzyme-linked immunosorbent assays intended for rapid detection of antimicrobial residues in bovine milk. In this study, the LacTek test protocol was modified for use with extracts of bovine tissue to detect beta-lactam, tetracycline, and sulfamethazine residues. Test performance characteristics--precision, accuracy, ruggedness, practicability, and analytical specificity and sensitivity--were investigated. Results suggest that LacTek tests can be easily adapted to detect antimicrobial residues in extracts of lean ground beef. However, positive samples may not contain residues at violative concentrations (i.e., Canadian maximum residue limits), and therefore, additional analysis would be required for final confirmation and quantitation (e.g., chromatography).

Soft X-ray observations of two BL Lacertae objects - Markarian 421 and 501

NASA Technical Reports Server (NTRS)

Singh, K. P.; Garmire, G. P.

1985-01-01

This paper reports on the soft X-ray (0.15-2.8 keV) observations of two BL Lacertae-type objects, viz., Mrk 421 and Mrk 501. The observations were made with the low-energy detectors on the HEAO 1 satellite during the period 1977 August-1978 December. Steep, soft X-ray power-law spectra with photon index Gamma = 3 are found for both Mrk 421 and Mrk 501. The power-law models are found to give a significantly better fit than the thermal models to the observed pulse-height spectra of Mrk 421 and Mrk 501. Day-to-day soft X-ray (0.25 keV band) intensity variations are observed only in Mrk 501. No significant change is found in Gamma from both the BL Lac objects during the period of observations. However, the sum of all the X-ray observations from 1976 until 1980 can be understood in terms of two spectral components of variable intensity to account for the X-ray emission observed between 0.15 and 20 keV from Mrk 421 and Mrk 501.

High-frequency predictions for number counts and spectral properties of extragalactic radio sources. New evidence of a break at mm wavelengths in spectra of bright blazar sources

NASA Astrophysics Data System (ADS)

Tucci, M.; Toffolatti, L.; de Zotti, G.; Martínez-González, E.

2011-09-01

We present models to predict high-frequency counts of extragalactic radio sources using physically grounded recipes to describe the complex spectral behaviour of blazars that dominate the mm-wave counts at bright flux densities. We show that simple power-law spectra are ruled out by high-frequency (ν ≥ 100 GHz) data. These data also strongly constrain models featuring the spectral breaks predicted by classical physical models for the synchrotron emission produced in jets of blazars. A model dealing with blazars as a single population is, at best, only marginally consistent with data coming from current surveys at high radio frequencies. Our most successful model assumes different distributions of break frequencies, νM, for BL Lacs and flat-spectrum radio quasars (FSRQs). The former objects have substantially higher values of νM, implying that the synchrotron emission comes from more compact regions; therefore, a substantial increase of the BL Lac fraction at high radio frequencies and at bright flux densities is predicted. Remarkably, our best model is able to give a very good fit to all the observed data on number counts and on distributions of spectral indices of extragalactic radio sources at frequencies above 5 and up to 220 GHz. Predictions for the forthcoming sub-mm blazar counts from Planck, at the highest HFI frequencies, and from Herschel surveys are also presented. Appendices are available in electronic form at http://www.aanda.org

Radio and γ -Ray Variability in the BL Lac PKS 0219−164: Detection of Quasi-periodic Oscillations in the Radio Light Curve

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhatta, Gopal, E-mail: gopalbhatta716@gmail.com; Mt. Suhora Observatory, Pedagogical University, ul. Podchorazych 2, 30-084 Kraków

In this work, we explore the long-term variability properties of the blazar PKS 0219−164 in the radio and the γ -ray regime, utilizing the OVRO 15 GHz and the Fermi /LAT observations from the period 2008–2017. We found that γ -ray emission is more variable than the radio emission implying that γ -ray emission possibly originated in more compact regions while the radio emission represented continuum emission from the large-scale jets. Also, in the γ -ray, the source exhibited spectral variability, characterized by the softer-when-brighter trend, a less frequently observed feature in the high-energy emission by BL Lacs. In radio,more » using Lomb–Scargle periodogram and weighted wavelet z -transform, we detected a strong signal of quasi-periodic oscillation (QPO) with a periodicity of 270 ± 26 days with possible harmonics of 550 ± 42 and 1150 ± 157 day periods. At a time when detections of QPOs in blazars are still under debate, the observed QPO with high statistical significance (∼97%–99% global significance over underlying red-noise processes) and persistent over nearly 10 oscillations could make one of the strongest cases for the detection of QPOs in blazar light curves. We discuss various blazar models that might lead to the γ -ray and radio variability, QPO, and the achromatic behavior seen in the high-energy emission from the source.« less

TOMOGRAPHY OF THE FERMI-LAT γ-RAY DIFFUSE EXTRAGALACTIC SIGNAL VIA CROSS CORRELATIONS WITH GALAXY CATALOGS

DOE PAGES

Xia, Jun-Qing; Cuoco, Alessandro; Branchini, Enzo; ...

2015-03-24

Building on our previous cross-correlation analysis (Xia et al. 2011) between the isotropic γ-ray background (IGRB) and different tracers of the large-scale structure of the universe, we update our results using 60 months of data from the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope (Fermi). For this study, we perform a cross-correlation analysis both in configuration and spherical harmonics space between the IGRB and objects that may trace the astrophysical sources of the IGRB: QSOs in the Sloan Digital Sky Survey (SDSS) DR6, the SDSS DR8 Main Galaxy Sample, luminous red galaxies (LRGs) in the SDSS catalog, infrared-selected galaxies in the Two Micron All Sky Survey (2MASS), and radio galaxies in the NRAO VLA Sky Survey (NVSS). The benefit of correlating the Fermi-LAT signal with catalogs of objects at various redshifts is to provide tomographic information on the IGRB, which is crucial in separating the various contributions and clarifying its origin. The main result is that, unlike in our previous analysis, we now observe a significant (>3.5σ) cross-correlation signal on angular scales smaller than 1° in the NVSS, 2MASS, and QSO cases and, at lower statistical significance (~3.0σ), with SDSS galaxies. The signal is stronger in two energy bands, E > 0.5 GeV and E > 1 GeV, but it is also seen at E > 10 GeV. No cross-correlation signal is detected between Fermi data and the LRGs. These results are robust against the choice of the statistical estimator, estimate of errors, map cleaning procedure, and instrumental effects. Finally, we test the hypothesis that the IGRB observed by Fermi-LAT originates from the summed contributions of three types of unresolved extragalactic sources: BL Lacertae objects (BL Lacs), flat spectrum radio quasars (FSRQs), and star-forming galaxies (SFGs). Finally, we find that a model in which the IGRB is mainly produced by SFGs (more » $$72_{-37}^{+23}$$% with 2σ errors), with BL Lacs and FSRQs giving a minor contribution, provides a good fit to the data. We also consider a possible contribution from misaligned active galactic nuclei, and we find that, depending on the details of the model and its uncertainty, they can also provide a substantial contribution, partly degenerate with the SFG one.« less

Fermi LAT detection of renewed GeV flaring activity from PKS 0426-380

NASA Astrophysics Data System (ADS)

Ciprini, Stefano

2012-12-01

The Large Area Telescope (LAT), one of the two instruments on the Fermi Gamma-ray Space Telescope, has observed gamma-ray flaring activity from a source positionally consistent with the BL Lac object PKS 0426-380 (also known as 2FGL J0428.6-3756, Nolan et al. 2012, ApJS, 199, 31, and RX J0428.6-3756) with radio coordinates R.A.: 67.16843 deg, Dec: -37.93877 deg, J2000 (Johnston et al. 1995, AJ, 110, 880) and redshift z=1.111 (Heidt et al....

Voids as alternatives to dark energy and the propagation of γ rays through the universe.

PubMed

DeLavallaz, Arnaud; Fairbairn, Malcolm

2012-04-27

We test the opacity of a void universe to TeV energy γ rays having obtained the extragalactic background light in that universe using a simple model and the observed constraints on the star formation rate history. We find that the void universe has significantly more opacity than a Λ cold dark matter universe, putting it at odds with observations of BL-Lac objects. We argue that while this method of distinguishing between the two cosmologies contains uncertainties, it circumvents any debates over fine-tuning.

Observations of blazars with ASCA

NASA Technical Reports Server (NTRS)

Makino, F.; Edelson, R.; Fujimoto, R.; Kii, T.; Idesawa, E.; Makishima, K.; Takahashi, T.; Sasaki, K.; Kamae, T.; Kubo, H.;

Measuring persistent temporomandibular joint nociception in rats and two mice strains.

PubMed

Kramer, Phillip R; Kerins, Carolyn A; Schneiderman, Emet; Bellinger, Larry L

2010-04-19

Temporomandibular joint (TMJ) pain has been reported to last for prolonged periods in humans. In rodents a variety of methods have been used to measure TMJ nociception, but for most of these methods the period of measurement has been minutes to a couple of hours. In addition, most measurement protocols required restraint or training of the animal. Previous studies from our laboratory demonstrated that feeding behavior, particularly meal duration, was an indicator of TMJ nociception in unrestrained and untrained male and female Sprague-Dawley rats for up to two days. In this study, we first found that injection of complete Freund's adjuvant (CFA) into the TMJ of rats significantly lengthened meal duration for 19 days and also decreased meal frequency for 42 days. Interestingly, the meal duration varied significantly from day to day within the 19 day period. TMJ interleukin-1 beta (IL-1 beta) and calcitonin gene-related peptide (CGRP) were significantly elevated in the TMJ tissues of CFA-injected animals and the level of these markers was attenuated as the meal duration decreased with time. Control animals injected with saline into the TMJ or CFA into the knee did not show a significant lengthening in meal duration but did show a decrease in meal frequency. In a second study, DBA/1LacJ mice given TMJ CFA injections showed a significantly lengthened meal duration on four of the seven days measured using end-of-the meal definition of 5 or 10 min. No other meal pattern changed significantly. Two days post-CFA injection, the DBA/1LacJ mice showed significantly elevated interleukin-6 (IL-6), but not elevated IL-1 beta. Seven days post-injection, both IL-6 and IL-1 beta were significantly elevated. No change in CGRP was detected. In this study C57Bl/6 mice also received TMJ CFA injections, but they did not show a lengthening in any meal pattern or significant increases in IL-1 beta, IL-6 or CGRP. Our data show, for the first time, that meal duration can be used to measure CFA-induced nociception in the TMJ over the course of several weeks in unrestrained rats and for up to seven days in the DBA/1LacJ mouse strain. In addition, C57Bl/6 mice are resistant to CFA-induced TMJ nociception at the same dose used in the DBA/1LacJ mice. (c) 2010 Elsevier Inc. All rights reserved.

Jet Precession Driven by a Supermassive Black Hole Binary System in the BL Lac Object PG 1553+113

NASA Astrophysics Data System (ADS)

Caproni, Anderson; Abraham, Zulema; Motter, Juliana Cristina; Monteiro, Hektor

2017-12-01

The recent discovery of a roughly simultaneous periodic variability in the light curves of the BL Lac object PG 1553+113 at several electromagnetic bands represents the first case of such odd behavior reported in the literature. Motivated by this, we analyzed 15 GHz interferometric maps of the parsec-scale radio jet of PG 1553+113 to verify the presence of a possible counterpart of this periodic variability. We used the Cross-entropy statistical technique to obtain the structural parameters of the Gaussian components present in the radio maps of this source. We kinematically identified seven jet components formed coincidentally with flare-like features seen in the γ-ray light curve. From the derived jet component positions in the sky plane and their kinematics (ejection epochs, proper motions, and sky position angles), we modeled their temporal changes in terms of a relativistic jet that is steadily precessing in time. Our results indicate a precession period in the observer’s reference frame of 2.24 ± 0.03 years, compatible with the periodicity detected in the light curves of PG 1553+113. However, the maxima of the jet Doppler boosting factor are systematically delayed relative to the peaks of the main γ-ray flares. We propose two scenarios that could explain this delay, both based on the existence of a supermassive black hole binary system in PG 1553+113. We estimated the characteristics of this putative binary system that also would be responsible for driving the inferred jet precession.

Long-term spectral and temporal behavior of the high-frequency peaked BL LAC object 1ES 1959+650

NASA Astrophysics Data System (ADS)

Backes, M.; Uellenbeck, M.; Hayashida, M.; Satalecka, K.; Tescaro, D.; Terzić, T.; MAGIC Collaboration; Fuhrmann, L.; Nestoras, I.; F-GAMMA project; Lähteenmäki, A.; Tornikoski, M.; Nieppola, E.; Metsähovi; Böttcher, M.; Collmar, W.; Weidinger, M.

2012-12-01

The high-frequency peaked BL Lac object 1ES 1959+650 is well-known for an exceptional outburst, which was observed at very high energy (VHE) γ-rays by the Whipple 10m and HEGRA telescopes in 2002. Remarkably, this outburst lacked associated X-ray emission (a socalled "orphan flare") and by this cannot easily be described by standard Synchrotron Self Compton (SSC) models. Models based on hadronic emission processes have also been proposed to explain the observed behavior. Subsequent multi-wavelength observations during a low flux state at TeV energies in 2006 can, instead, be explained by a standard single-zone SSC model. In this context, 1ES 1959+650 has been regularly monitored by the MAGIC telescope since 2005. During these years, no significant variation in the VHE γ-ray flux has been observed. The low energy part of this is in very good agreement with the high-energy part of the time-integrated energy spectrum as measured by Fermi-LAT. Based on this constant flux level in VHE γ-rays, we assembled the time-integrated spectral energy distribution (SED) of 1ES 1959+650 from radio to VHE γ-rays. Despite the non-variability at very high energies, significant flux and spectral variations have been observed at optical and X-ray frequencies in the meanwhile. Furthermore, the shape of the SED at high energy γ-rays as measured by Fermi-LAT is essentially flat which cannot be explained by either conventional single-zone SSC models, or models invoking external radiation fields (EC).

Characterizing the γ-ray long-term variability of PKS 2155-304 with H.E.S.S. and Fermi-LAT

NASA Astrophysics Data System (ADS)

H.E.S.S. Collaboration; Abdalla, H.; Abramowski, A.; Aharonian, F.; Ait Benkhali, F.; Akhperjanian, A. G.; Andersson, T.; Angüner, E. O.; Arrieta, M.; Aubert, P.; Backes, M.; Balzer, A.; Barnard, M.; Becherini, Y.; Becker Tjus, J.; Berge, D.; Bernhard, S.; Bernlöhr, K.; Blackwell, R.; Böttcher, M.; Boisson, C.; Bolmont, J.; Bordas, P.; Bregeon, J.; Brun, F.; Brun, P.; Bryan, M.; Bulik, T.; Capasso, M.; Carr, J.; Casanova, S.; Cerruti, M.; Chakraborty, N.; Chalme-Calvet, R.; Chaves, R. C. G.; Chen, A.; Chevalier, J.; Chrétien, M.; Colafrancesco, S.; Cologna, G.; Condon, B.; Conrad, J.; Cui, Y.; Davids, I. D.; Decock, J.; Degrange, B.; Deil, C.; Devin, J.; deWilt, P.; Dirson, L.; Djannati-Ataï, A.; Domainko, W.; Donath, A.; Drury, L. O.'C.; Dubus, G.; Dutson, K.; Dyks, J.; Edwards, T.; Egberts, K.; Eger, P.; Ernenwein, J.-P.; Eschbach, S.; Farnier, C.; Fegan, S.; Fernandes, M. V.; Fiasson, A.; Fontaine, G.; Förster, A.; Funk, S.; Füßling, M.; Gabici, S.; Gajdus, M.; Gallant, Y. A.; Garrigoux, T.; Giavitto, G.; Giebels, B.; Glicenstein, J. F.; Gottschall, D.; Goyal, A.; Grondin, M.-H.; Hadasch, D.; Hahn, J.; Haupt, M.; Hawkes, J.; Heinzelmann, G.; Henri, G.; Hermann, G.; Hervet, O.; Hinton, J. A.; Hofmann, W.; Hoischen, C.; Holler, M.; Horns, D.; Ivascenko, A.; Jacholkowska, A.; Jamrozy, M.; Janiak, M.; Jankowsky, D.; Jankowsky, F.; Jingo, M.; Jogler, T.; Jouvin, L.; Jung-Richardt, I.; Kastendieck, M. A.; Katarzyński, K.; Katz, U.; Kerszberg, D.; Khélifi, B.; Kieffer, M.; King, J.; Klepser, S.; Klochkov, D.; Kluźniak, W.; Kolitzus, D.; Komin, Nu.; Kosack, K.; Krakau, S.; Kraus, M.; Krayzel, F.; Krüger, P. P.; Laffon, H.; Lamanna, G.; Lau, J.; Lees, J.-P.; Lefaucheur, J.; Lefranc, V.; Lemière, A.; Lemoine-Goumard, M.; Lenain, J.-P.; Leser, E.; Lohse, T.; Lorentz, M.; Liu, R.; López-Coto, R.; Lypova, I.; Marandon, V.; Marcowith, A.; Mariaud, C.; Marx, R.; Maurin, G.; Maxted, N.; Mayer, M.; Meintjes, P. J.; Meyer, M.; Mitchell, A. M. W.; Moderski, R.; Mohamed, M.; Mohrmann, L.; Morå, K.; Moulin, E.; Murach, T.; de Naurois, M.; Niederwanger, F.; Niemiec, J.; Oakes, L.; O'Brien, P.; Odaka, H.; Öttl, S.; Ohm, S.; Ostrowski, M.; Oya, I.; Padovani, M.; Panter, M.; Parsons, R. D.; Pekeur, N. W.; Pelletier, G.; Perennes, C.; Petrucci, P.-O.; Peyaud, B.; Piel, Q.; Pita, S.; Poon, H.; Prokhorov, D.; Prokoph, H.; Pühlhofer, G.; Punch, M.; Quirrenbach, A.; Raab, S.; Reimer, A.; Reimer, O.; Renaud, M.; de los Reyes, R.; Rieger, F.; Romoli, C.; Rosier-Lees, S.; Rowell, G.; Rudak, B.; Rulten, C. B.; Sahakian, V.; Salek, D.; Sanchez, D. A.; Santangelo, A.; Sasaki, M.; Schlickeiser, R.; Schüssler, F.; Schulz, A.; Schwanke, U.; Schwemmer, S.; Settimo, M.; Seyffert, A. S.; Shafi, N.; Shilon, I.; Simoni, R.; Sol, H.; Spanier, F.; Spengler, G.; Spies, F.; Stawarz, Ł.; Steenkamp, R.; Stegmann, C.; Stinzing, F.; Stycz, K.; Sushch, I.; Tavernet, J.-P.; Tavernier, T.; Taylor, A. M.; Terrier, R.; Tibaldo, L.; Tiziani, D.; Tluczykont, M.; Trichard, C.; Tuffs, R.; Uchiyama, Y.; van der Walt, D. J.; van Eldik, C.; van Rensburg, C.; van Soelen, B.; Vasileiadis, G.; Veh, J.; Venter, C.; Viana, A.; Vincent, P.; Vink, J.; Voisin, F.; Völk, H. J.; Vuillaume, T.; Wadiasingh, Z.; Wagner, S. J.; Wagner, P.; Wagner, R. M.; White, R.; Wierzcholska, A.; Willmann, P.; Wörnlein, A.; Wouters, D.; Yang, R.; Zabalza, V.; Zaborov, D.; Zacharias, M.; Zdziarski, A. A.; Zech, A.; Zefi, F.; Ziegler, A.; Żywucka, N.

2017-02-01

Studying the temporal variability of BL Lac objects at the highest energies provides unique insights into the extreme physical processes occurring in relativistic jets and in the vicinity of super-massive black holes. To this end, the long-term variability of the BL Lac object PKS 2155-304 is analyzed in the high (HE, 100 MeV < E < 300 GeV) and very high energy (VHE, E > 200 GeV) γ-ray domain. Over the course of 9 yr of H.E.S.S. observations the VHE light curve in the quiescent state is consistent with a log-normal behavior. The VHE variability in this state is well described by flicker noise (power-spectral-density index ) on timescales larger than one day. An analysis of 5.5 yr of HE Fermi-LAT data gives consistent results (, on timescales larger than 10 days) compatible with the VHE findings. The HE and VHE power spectral densities show a scale invariance across the probed time ranges. A direct linear correlation between the VHE and HE fluxes could neither be excluded nor firmly established. These long-term-variability properties are discussed and compared to the red noise behavior (β 2) seen on shorter timescales during VHE-flaring states. The difference in power spectral noise behavior at VHE energies during quiescent and flaring states provides evidence that these states are influenced by different physical processes, while the compatibility of the HE and VHE long-term results is suggestive of a common physical link as it might be introduced by an underlying jet-disk connection.

Lupus anticoagulant: a multicenter study for a standardized and harmonized reporting.

PubMed

Poz, Alessandra; Pradella, Paola; Azzarini, Gabriella; Santarossa, Liliana; Bardin, Cristina; Zardo, Lorena; Giacomello, Roberta

2016-03-01

Laboratory assessment of Lupus anticoagulant (LAC) is very challenging because of inter and intralaboratory variability, which makes it difficult to standardize and harmonize results expression. Five hospital laboratories in North-eastern Italy shared their efforts and their experience in a cross-laboratory study, conducting the diagnostic process as homogeneously as possible and providing a better interpretation for LAC positivity. Hundred normal samples from healthy subjects (20 from each center) were processed to confirm negative upper limits and calculate positivity cutoffs of LAC integrated assays, that is dilute Russell's viper venom time (dRVVT) and silica clotting time (SCT). Moreover, 311 samples previously diagnosed by the laboratories as positive for LAC were analyzed to characterize different positivity levels for each assay. As far as the analysis of healthy subjects is concerned, negative upper limits are set at 1.17 and 1.19 for dRVVT and SCT screen ratio, respectively. Positivity cutoffs are set at 1.20 for dRVVT and 1.23 for SCT, expressed as Test Ratio calculated on screen and confirm integrated tests. Positive results for each integrated assay are subsequently divided into three subgroups: weak, moderate and strong; the results obtained are presented as a score proposal that can provide LAC interpretation. The combined use of both dRVVT and SCT assays and the definition of different positivity levels may lead to clearer, more objective LAC reporting. An interpretative table for LAC-proposed score provides LAC-positive results and it is now adopted by all centers involved in the study.

Light absorbing carbon emissions from commercial shipping

NASA Astrophysics Data System (ADS)

Lack, Daniel; Lerner, Brian; Granier, Claire; Baynard, Tahllee; Lovejoy, Edward; Massoli, Paola; Ravishankara, A. R.; Williams, Eric

2008-07-01

Extensive measurements of the emission of light absorbing carbon aerosol (LAC) from commercial shipping are presented. Vessel emissions were sampled using a photoacoustic spectrometer in the Gulf of Mexico region. The highest emitters (per unit fuel burnt) are tug boats, thus making significant contributions to local air quality in ports. Emission of LAC from cargo and non cargo vessels in this study appears to be independent of engine load. Shipping fuel consumption data (2001) was used to calculate a global LAC contribution of 133(+/-27) Ggyr-1, or ~1.7% of global LAC. This small fraction could have disproportionate effects on both air quality near port areas and climate in the Arctic if direct emissions of LAC occur in that region due to opening Arctic sea routes. The global contribution of this LAC burden was investigated using the MOZART model. Increases of 20-50 ng m-3 LAC (relative increases up to 40%) due to shipping occur in the tropical Atlantic, Indonesia, central America and the southern regions of South America and Africa.

Radio Telescope Reveals Secrets of Massive Black Hole

NASA Astrophysics Data System (ADS)

2008-04-01

At the cores of many galaxies, supermassive black holes expel powerful jets of particles at nearly the speed of light. Just how they perform this feat has long been one of the mysteries of astrophysics. The leading theory says the particles are accelerated by tightly-twisted magnetic fields close to the black hole, but confirming that idea required an elusive close-up view of the jet's inner throat. Now, using the unrivaled resolution of the National Radio Astronomy Observatory's Very Long Baseline Array (VLBA), astronomers have watched material winding a corkscrew outward path and behaving exactly as predicted by the theory. Galactic core and jet Artist's conception of region near supermassive black hole where twisted magnetic fields propel and shape jet of particles (Credit: Marscher et al., Wolfgang Steffen, Cosmovision, NRAO/AUI/NSF). Click on image for high-resolution file. Watch Video of Black-Hole-Powered Jet (Credit: Cosmovision, Wolfgang Steffen) Download: NTSC Format (90MB) | PAL Format (90MB) "We have gotten the clearest look yet at the innermost portion of the jet, where the particles actually are accelerated, and everything we see supports the idea that twisted, coiled magnetic fields are propelling the material outward," said Alan Marscher, of Boston University, leader of an international research team. "This is a major advance in our understanding of a remarkable process that occurs throughout the Universe," he added. Marscher's team studied a galaxy called BL Lacertae (BL Lac), some 950 million light-years from Earth. BL Lac is a blazar, the most energetic type of black-hole-powered galactic core. A black hole is a concentration of mass so dense that not even light can escape its gravitational pull. Supermassive black holes in galaxies' cores power jets of particles and intense radiation in similar objects including quasars and Seyfert galaxies. Material pulled inward toward the black hole forms a flattened, rotating disk, called an accretion disk. As the material moves from the outer edge of the disk inward, magnetic field lines perpendicular to the disk are twisted, forming a tightly-coiled bundle that, astronomers believe, propels and confines the ejected particles. Closer to the black hole, space itself, including the magnetic fields, is twisted by the strong gravitational pull and rotation of the black hole. Theorists predicted that material moving outward in this close-in acceleration region would follow a corkscrew-shaped path inside the bundle of twisted magnetic fields. They also predicted that light and other radiation emitted by the moving material would brighten when its rotating path was aimed most directly toward Earth. Marscher and his colleagues predicted there would also be a flare later when the material hits a stationary shock wave called the "core" some time after it has emerged from the acceleration region. "That behavior is exactly what we saw," Marscher said, when his team followed an outburst from BL Lac. In late 2005 and early 2006, the astronomers watched BL Lac with an international collection of telescopes as a knot of material was ejected outward through the jet. As the material sped out from the neighborhood of the black hole, the VLBA could pinpoint its location, while other telescopes measured the properties of the radiation emitted from the knot. Bright bursts of light, X-rays, and gamma rays came when the knot was precisely at locations where the theories said such bursts would be seen. In addition, the alignment of the radio and light waves -- a property called polarization -- rotated as the knot wound its corkscrew path inside the tight throat of twisted magnetic fields. "We got an unprecedented view of the inner portion of one of these jets and gained information that's very important to understanding how these tremendous particle accelerators work," Marscher said. In addition to the continent-wide VLBA, an array of 10 radio telescopes spread from Hawaii to the Virgin Islands, the team used telescopes at the Steward Observatory, the Crimean Astrophysical Observatory, Lowell Observatory, Perugia University Astronomical Observatory, Abastumani Astrophysical Observatory, NASA's Rossi X-Ray Timing Explorer, the University of Michigan Radio Astronomy Observatory, and the Metsahovi Radio Observatory. The astronomers reported their findings in the April 24 issue of the journal Nature. The National Radio Astronomy Observatory is a facility of the National Science Foundation, operated under cooperative agreement by Associated Universities, Inc.

Fermi-LAT and Swift-XRT observe exceptionally high activity from the nearby TeV blazar Mrk421

NASA Astrophysics Data System (ADS)

Paneque, D.; D'Ammando, F.; Orienti, M.; Falcon, A.

2013-04-01

The high-synchrotron-peaked BL Lac Mrk421 (also known as 2FGL J1104.4+3812, Nolan et al. 2012, ApJS, 199, 31; R.A.= 11h04m27.3139s, Dec.= +38d12m31.799s, J2000.0, Fey et al. 2004, AJ, 127, 3587), at redshift z=0.03, is the subject of an extensive multi-year and multi-instrument program that aims at characterizing with exquisite detail the temporal evolution of the blazar emission across the electromagnetic spectrum.

Optical Spectra of Four Objects Identified with Variable Radio Sources

NASA Astrophysics Data System (ADS)

Chavushyan, V.; Mujica, R.; Gorshkov, A. G.; Konnikova, V. K.; Mingaliev, M. G.

2000-06-01

We obtained optical spectra of four objects identified with variable radio sources. Three objects (0029+0554, 0400+0550, 2245+0500) were found to be quasars with redshifts of 1.314, 0.761, and 1.091. One object (2349+0534) has a continuum spectrum characteristic of BL Lac objects. We analyze spectra of the radio sources in the range 0.97-21.7 GHz for the epoch 1997 and in the range 3.9-11.1 GHz for the epoch 1990, as well as the pattern of variability of their flux densities on time scales of 1.5 and 7 years.

The X-ray spectra of blazars: Analysis of the complete EXOSAT archive

NASA Technical Reports Server (NTRS)

Sambruna, Rita M.; Barr, Paul; Giommi, Paolo; Maraschi, Laura; Tagliaferri, Gianpiero; Treves, Aldo

1994-01-01

We analyzed the 0.1-10 keV spectra of 26 blazars (21 BL Lac objects and 5 highly polarized quasars), on the basis of 93 exposures taken from the EXOSAT archives. Fits were performed first with a single power-law model. Indications are found that better fits can be obtained with models where the slope steepens at higher energies. We therefore considered a broken power law and found that in a large fraction of the spectra the fit is significantly improved. Fits with a power law + oxygen edge at 0.6 keV are also explored.

VizieR Online Data Catalog: Radio-loud AGN with relativistic jets (Olguin-Iglesias+, 2016)

NASA Astrophysics Data System (ADS)

Olguin-Iglesias, A.; Leon-Tavares, J.; Kotilainen, J. K.; Chavushyan, V.; Tornikoski, M.; Valtaoja, E.; Anorve, C.; Valdes, J.; Carrasco, L.

2017-11-01

The sample of sources analysed in this work is a sub-sample of variable radio-loud AGN monitored at 7mm (S7mm>1Jy) with the Aalto University Metsahovi Radio Observatory, in Finland (http://metsahovi.aalto.fi/en/) since the last 30 years (Terasranta et al., 1992A&AS...94..121T; Teraesranta et al., 1998, Cat. J/A+AS/132/305; Leon-Tavares et al., 2011A&A...532A.146L; Nieppola et al., 2011, Cat. J/A+A/535/A69). According to the AGN unification scheme (Antonucci, 1993ARA&A..31..473A; Urry & Padovani, 1995PASP..107..803U), FSRQ and BL Lacs are those AGN whose relativistic jets point towards the Earth. Observations were made with the Nordic Optical Telescope (NOT) at La Roque de los Muchachos, La Palma, Canarias, Spain. They were conducted between 2011 May 09 and September 15 using the near-infrared Camera (NOTCam)3 on the NOT. NOTcam field of view is 4'x4' with a pixel scale of 0.234-arcsec/pixel designed to be used in the range from 0.8 to 2.5um in the bands J, H and K. (1 data file).

Long-term monitoring of PKS 0537-441 with Fermi-LAT and multiwavelength observations

DOE PAGES

D'Ammando, F.; Antolini, E.; Tosti, G.; ...

2013-03-20

Here, we report on multiwavelength observations of the blazar PKS 0537-441 (z = 0.896) obtained from microwaves through γ-rays by Submillimeter Array, Rapid Eye Mounting, Automatic Telescope for Optical Monitoring (ATOM), Swift and Fermi mostly during 2008 August–2010 April. Strong variability has been observed in γ-rays, with two major flaring episodes (2009 July and 2010 March) and a harder-when-brighter behaviour, quite common for flat spectrum radio quasars and low-synchrotron-peaked BL Lacertae objects (BL Lacs), in 2010 March. In the same way, the spectral energy distribution (SED) of the source cannot be modelled by a simple synchrotron self-Compton model, as opposedmore » to many BL Lacs, but the addition of an external Compton component of seed photons from a dust torus is needed. The 230 GHz light curve showed an increase simultaneous with the γ-ray one, indicating co-spatiality of the mm and γ-ray emission region likely at large distance from the central engine. The low, average, and high activity SED of the source could be fit changing only the electron distribution parameters, but two breaks in the electron distribution are necessary. The ensuing extra spectral break, located at near-infrared (NIR)–optical frequencies, together with that in γ-rays seem to indicate a common origin, most likely due to an intrinsic feature in the underlying electron distribution. An overall correlation between the γ-ray band with the R band and K band has been observed with no significant time lag. On the other hand, when inspecting the light curves on short time-scales some differences are evident. In particular, flaring activity has been detected in NIR and optical bands with no evident γ-ray counterparts in 2009 September and November. Moderate variability has been observed in X-rays with no correlation between flux and photon index. Finally, an increase of the detected X-ray flux with no counterpart at the other wavelengths has been observed in 2008 October, suggesting once more a complex correlation between the emission at different energy bands.« less

Observations and analysis activities of the International Ultraviolet Explorer satellite telescope

NASA Technical Reports Server (NTRS)

Shull, J. Michael

1996-01-01

The funds from this grant were used to support observations and analysis with the International Ultraviolet Explorer (IUE) satellite telescope. The main area of scientific research concerned the variability analyses of ultraviolet spectra of Active Galactic Nuclei, primarily quasars, Seyfert galaxies, and BL Lacertae objects. The Colorado group included, at various times, the P.I. (J.M. Shull), Research Associate Dr. Rick Edelson, and graduate students Jon Saken, Elise Sachs, and Steve Penton. A portion of the work was also performed by CU undergraduate student Cheong-ming Fu. A major product of the effort was a database of all IUE spectra of active galactic nuclei. This database is being analyzed to obtain spectral indices, line fluxes, and continuum fluxes for over 500 AGN. As a by-product of this project, we implemented a new, improved technique of spectral extraction of IUE spectra, which has been used in several AGN-WATCH campaigns (on the Seyfert galaxy NGC 4151 and on the BL Lac object PKS 2155-304).

A Multiwavelength Study of Flaring Activity in the High-energy Peaked BL Lac Object 1ES 1959+650 During 2015–2016

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaur, Navpreet; Chandra, S.; Baliyan, Kiran S

We present the results from a multiwavelength study of the flaring activity in the high-energy peaked BL Lac object 1ES 1959+650 during 2015 January–2016 June. The source underwent two major outbursts, during 2015 March and 2015 October, across the whole electromagnetic spectrum (EMS). We used data from Fermi -LAT and Swift -XRT/UVOT, and optical data from Mt. Abu InfraRed Observatory and Steward Observatory to look for possible correlations between the emissions at different energies and the nature of the variability during the flaring state. During the 2015 October outburst, the nightly averaged V -band brightest magnitude, 14.45(0.03), and faintest magnitude,more » 14.45(0.03), were recorded. Apart from long-term flares, rapid and short-term variabilities were noticed at all energies. Our study suggests that the flaring activities at all frequencies, with diverse flare durations and time lags, are correlated. The magnetic field strength is estimated to be 4 G using the synchrotron-cooling timescale (2.3 hr), and the upper limits on the sizes of both emission regions, γ -ray and optical, are estimated to be of the order of 10{sup 16} cm. The quasi-simultaneous flux enhancements in 15 GHz and VHE γ -ray emissions indicate a fresh injection of plasma into the jet, which interacts with a standing submillimeter core, resulting in co-spatial emissions across the EMS. The synchrotron peak shifts to higher frequency in the spectral energy distribution while the γ -ray spectra softens during the flaring. The shape of the inverse-Compton spectra indicates a change in the particle energy distribution pre- and post-flare.« less

First NuSTAR Observations of the BL Lac-Type Blazar PKS 2155-304: Constraints on the Jet Content and Distribution of Radiating Particles

NASA Technical Reports Server (NTRS)

Madejski, G. M.; Nalewajko, K.; Madsen, K. K.; Chiang, J.; Balokovic, M.; Paneque, D.; Furniss, A. K.; Hayashida, M.; Urry, C. M.; Sikora, M.;

A Multiwavelength Study of Flaring Activity in the High-energy Peaked BL Lac Object 1ES 1959+650 During 2015-2016

NASA Astrophysics Data System (ADS)

Kaur, Navpreet; Chandra, S.; Baliyan, Kiran S.; Sameer; Ganesh, S.

2017-09-01

We present the results from a multiwavelength study of the flaring activity in the high-energy peaked BL Lac object 1ES 1959+650 during 2015 January-2016 June. The source underwent two major outbursts, during 2015 March and 2015 October, across the whole electromagnetic spectrum (EMS). We used data from Fermi-LAT and Swift-XRT/UVOT, and optical data from Mt. Abu InfraRed Observatory and Steward Observatory to look for possible correlations between the emissions at different energies and the nature of the variability during the flaring state. During the 2015 October outburst, the nightly averaged V-band brightest magnitude, 14.45(0.03), and faintest magnitude, 14.45(0.03), were recorded. Apart from long-term flares, rapid and short-term variabilities were noticed at all energies. Our study suggests that the flaring activities at all frequencies, with diverse flare durations and time lags, are correlated. The magnetic field strength is estimated to be 4 G using the synchrotron-cooling timescale (2.3 hr), and the upper limits on the sizes of both emission regions, γ-ray and optical, are estimated to be of the order of 1016 cm. The quasi-simultaneous flux enhancements in 15 GHz and VHE γ-ray emissions indicate a fresh injection of plasma into the jet, which interacts with a standing submillimeter core, resulting in co-spatial emissions across the EMS. The synchrotron peak shifts to higher frequency in the spectral energy distribution while the γ-ray spectra softens during the flaring. The shape of the inverse-Compton spectra indicates a change in the particle energy distribution pre- and post-flare.

First NuSTAR Observations of the BL Lac-type Blazar PKS 2155-304: Constraints on the Jet Content and Distribution of Radiating Particles

NASA Astrophysics Data System (ADS)

Madejski, G. M.; Nalewajko, K.; Madsen, K. K.; Chiang, J.; Baloković, M.; Paneque, D.; Furniss, A. K.; Hayashida, M.; Urry, C. M.; Sikora, M.; Ajello, M.; Blandford, R. D.; Harrison, F. A.; Sanchez, D.; Giebels, B.; Stern, D.; Alexander, D. M.; Barret, D.; Boggs, S. E.; Christensen, F. E.; Craig, W. W.; Forster, K.; Giommi, P.; Grefenstette, B.; Hailey, C.; Hornstrup, A.; Kitaguchi, T.; Koglin, J. E.; Mao, P. H.; Miyasaka, H.; Mori, K.; Perri, M.; Pivovaroff, M. J.; Puccetti, S.; Rana, V.; Westergaard, N. J.; Zhang, W. W.; Zoglauer, A.

2016-11-01

We report the first hard X-ray observations with NuSTAR of the BL Lac-type blazar PKS 2155-304, augmented with soft X-ray data from XMM-Newton and γ-ray data from the Fermi Large Area Telescope, obtained in 2013 April when the source was in a very low flux state. A joint NuSTAR and XMM spectrum, covering the energy range 0.5-60 keV, is best described by a model consisting of a log-parabola component with curvature β ={0.3}-0.1+0.2 and a (local) photon index 3.04 ± 0.15 at photon energy of 2 keV, and a hard power-law tail with photon index 2.2 ± 0.4. The hard X-ray tail can be smoothly joined to the quasi-simultaneous γ-ray spectrum by a synchrotron self-Compton component produced by an electron distribution with index p = 2.2. Assuming that the power-law electron distribution extends down to γ min = 1 and that there is one proton per electron, an unrealistically high total jet power of L p ˜ 1047 erg s-1 is inferred. This can be reduced by two orders of magnitude either by considering a significant presence of electron-positron pairs with lepton-to-proton ratio {n}{{e}+{{e}}-}/{n}{{p}}˜ 30, or by introducing an additional, low-energy break in the electron energy distribution at the electron Lorentz factor γ br1 ˜ 100. In either case, the jet composition is expected to be strongly matter-dominated.

Extracorporeal circulation as a new experimental pathway for myoblast implantation in mdx mice.

PubMed

Torrente, Y; D'Angelo, M G; Del Bo, R; DeLiso, A; Casati, R; Benti, R; Corti, S; Comi, G P; Gerundini, P; Anichini, A; Scarlato, G; Bresolin, N

1999-01-01

The deficiency of dystrophin, a sarcolemmal associated protein, is responsible for Duchenne muscular dystrophy (DMD). Gene replacement is attractive as a potential therapy. In this article, we describe a new method for myoblast transplantation and expression of dystrophin in skeletal muscle tissue of dystrophin-deficient mdx mouse through iliac vessels extracorporeal circulation. We evaluated the extracorporeal circulation as an alternative route of delivering myoblasts to the target tissue. Two series of experiments were performed with the extracorporeal circulation. In a first series, L6 rat myoblasts, transfected with LacZ reporter gene, were perfused in limbs of 15 rats. In the second series, the muscle limbs of three 6-8-week-old mdx were perfused with myoblasts of donor C57BL10J mice. Before these perfusions, the right tibialis anterior (TA) muscle of the rats and mdx was injected three times at several sites with bupivacaine (BPVC) and hyaluronidase. The ability of injected cells to migrate in the host tissue was assessed in rats by technetium-99m cell labeling. No radioactivity was detected in the lungs, bowels, and liver of animals treated with extracorporeal circulation. The tissue integration and viability of the myoblasts were ultimately confirmed by genetic and histochemical analysis of LacZ reporter gene. Following a single extracorporeal perfusion of myoblasts from donor C57BL10J, sarcolemmal expression of dystrophin was observed in clusters of myofibers in tibialis anterior muscles previously treated with BPVC and hyaluronidase. Furthermore, large clusters of dystrophin-positive fibers were observed in muscles up to 21 days after repeated treatments. These clusters represented an average of 4.2% of the total muscle fibers. These results demonstrate that the extracorporeal circulation allows selective myoblast-mediated gene transfer to muscles, opening new perspectives in muscular dystrophy gene therapy.

VERITAS Detection of γ-Ray Flaring Activity From the BL Lac Object 1ES 1727+502 During Bright Moonlight Observations

NASA Astrophysics Data System (ADS)

Archambault, S.; Archer, A.; Beilicke, M.; Benbow, W.; Bird, R.; Biteau, J.; Bouvier, A.; Bugaev, V.; Cardenzana, J. V.; Cerruti, M.; Chen, X.; Ciupik, L.; Connolly, M. P.; Cui, W.; Dickinson, H. J.; Dumm, J.; Eisch, J. D.; Errando, M.; Falcone, A.; Feng, Q.; Finley, J. P.; Fleischhack, H.; Fortin, P.; Fortson, L.; Furniss, A.; Gillanders, G. H.; Griffin, S.; Griffiths, S. T.; Grube, J.; Gyuk, G.; Håkansson, N.; Hanna, D.; Holder, J.; Humensky, T. B.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kertzman, M.; Khassen, Y.; Kieda, D.; Krause, M.; Krennrich, F.; Kumar, S.; Lang, M. J.; Maier, G.; McArthur, S.; McCann, A.; Meagher, K.; Millis, J.; Moriarty, P.; Mukherjee, R.; Nieto, D.; O'Faoláin de Bhróithe, A.; Ong, R. A.; Otte, A. N.; Park, N.; Pohl, M.; Popkow, A.; Prokoph, H.; Pueschel, E.; Quinn, J.; Ragan, K.; Reyes, L. C.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Santander, M.; Sembroski, G. H.; Shahinyan, K.; Smith, A. W.; Staszak, D.; Telezhinsky, I.; Tucci, J. V.; Tyler, J.; Varlotta, A.; Vincent, S.; Wakely, S. P.; Weinstein, A.; Welsing, R.; Wilhelm, A.; Williams, D. A.; Zitzer, B.; Veritas Collaboration; Hughes, Z. D.

2015-08-01

During moonlit nights, observations with ground-based Cherenkov telescopes at very high energies (VHEs, E\\gt 100 GeV) are constrained since the photomultiplier tubes (PMTs) in the telescope camera are extremely sensitive to the background moonlight. Observations with the VERITAS telescopes in the standard configuration are performed only with a moon illumination less than 35% of full moon. Since 2012, the VERITAS collaboration has implemented a new observing mode under bright moonlight, by either reducing the voltage applied to the PMTs (reduced-high-voltage; RHV configuration), or by utilizing UV-transparent filters. While these operating modes result in lower sensitivity and increased energy thresholds, the extension of the available observing time is useful for monitoring variable sources such as blazars and sources requiring spectral measurements at the highest energies. In this paper we report the detection of γ-ray flaring activity from the BL Lac object 1ES 1727+502 during RHV observations. This detection represents the first evidence of VHE variability from this blazar. The integral flux is (1.1+/- 0.2)× {10}-11 {{cm}}-2 {{{s}}}-1 above 250 GeV, which is about five times higher than the low-flux state. The detection triggered additional VERITAS observations during standard dark-time. Multiwavelength observations with the FLWO 48″ telescope, and the Swift and Fermi satellites are presented and used to produce the first spectral energy distribution (SED) of this object during γ-ray flaring activity. The SED is then fitted with a standard synchrotron-self-Compton model, placing constraints on the properties of the emitting region and of the acceleration mechanism at the origin of the relativistic particle population in the jet.

Multiwavelength Observations of the Previously Unidentified Blazar RX J0648.7+1516

NASA Astrophysics Data System (ADS)

Aliu, E.; Aune, T.; Beilicke, M.; Benbow, W.; Böttcher, M.; Bouvier, A.; Bradbury, S. M.; Buckley, J. H.; Bugaev, V.; Cannon, A.; Cesarini, A.; Ciupik, L.; Connolly, M. P.; Cui, W.; Decerprit, G.; Dickherber, R.; Duke, C.; Errando, M.; Falcone, A.; Feng, Q.; Finnegan, G.; Fortson, L.; Furniss, A.; Galante, N.; Gall, D.; Gillanders, G. H.; Godambe, S.; Griffin, S.; Grube, J.; Gyuk, G.; Hanna, D.; Hivick, B.; Holder, J.; Huan, H.; Hughes, G.; Hui, C. M.; Humensky, T. B.; Kaaret, P.; Karlsson, N.; Kertzman, M.; Kieda, D.; Krawczynski, H.; Krennrich, F.; Maier, G.; Majumdar, P.; McArthur, S.; McCann, A.; Moriarty, P.; Mukherjee, R.; Nelson, T.; Ong, R. A.; Orr, M.; Otte, A. N.; Park, N.; Perkins, J. S.; Pichel, A.; Pohl, M.; Prokoph, H.; Quinn, J.; Ragan, K.; Reyes, L. C.; Reynolds, P. T.; Roache, E.; Rose, H. J.; Ruppel, J.; Saxon, D. B.; Sembroski, G. H.; Skole, C.; Smith, A. W.; Staszak, D.; Tešić, G.; Theiling, M.; Thibadeau, S.; Tsurusaki, K.; Tyler, J.; Varlotta, A.; Vassiliev, V. V.; Wakely, S. P.; Weekes, T. C.; Weinstein, A.; Williams, D. A.; Zitzer, B.; VERITAS Collaboration; Ciprini, S.; Fumagalli, M.; Kaplan, K.; Paneque, D.; Prochaska, J. X.

2011-12-01

We report on the VERITAS discovery of very high energy (VHE) gamma-ray emission above 200 GeV from the high-frequency-peaked BL Lac (HBL) object RX J0648.7+1516 (GB J0648+1516), associated with 1FGL J0648.8+1516. The photon spectrum above 200 GeV is fitted by a power law dN/dE = F 0(E/E 0)-Γ with a photon index Γ of 4.4 ± 0.8stat ± 0.3syst and a flux normalization F 0 of (2.3 ± 0.5stat ± 1.2sys) × 10-11 TeV-1 cm-2 s-1 with E 0 = 300 GeV. No VHE variability is detected during VERITAS observations of RX J0648.7+1516 between 2010 March 4 and April 15. Following the VHE discovery, the optical identification and spectroscopic redshift were obtained using the Shane 3 m Telescope at the Lick Observatory, showing the unidentified object to be a BL Lac type with a redshift of z = 0.179. Broadband multiwavelength observations contemporaneous with the VERITAS exposure period can be used to subclassify the blazar as an HBL object, including data from the MDM observatory, Swift-UVOT, and X-Ray Telescope, and continuous monitoring at photon energies above 1 GeV from the Fermi Large Area Telescope (LAT). We find that in the absence of undetected, high-energy rapid variability, the one-zone synchrotron self-Compton (SSC) model overproduces the high-energy gamma-ray emission measured by the Fermi-LAT over 2.3 years. The spectral energy distribution can be parameterized satisfactorily with an external-Compton or lepto-hadronic model, which have two and six additional free parameters, respectively, compared to the one-zone SSC model.

The Ionization Source in the Nucleus of M84

NASA Technical Reports Server (NTRS)

Bower, G. A.; Green, R. F.; Quillen, A. C.; Danks, A.; Malumuth, E. M.; Gull, T.; Woodgate, B.; Hutchings, J.; Joseph, C.; Kaiser, M. E.

2000-01-01

We have obtained new Hubble Space Telescope (HST) observations of M84, a nearby massive elliptical galaxy whose nucleus contains a approximately 1.5 X 10(exp 9) solar mass dark compact object, which presumably is a supermassive black hole. Our Space Telescope Imaging Spectrograph (STIS) spectrum provides the first clear detection of emission lines in the blue (e.g., [0 II] lambda 3727, HBeta and [0 III] lambda lambda4959,5007), which arise from a compact region approximately 0".28 across centered on the nucleus. Our Near Infrared Camera and MultiObject Spectrometer (NICMOS) images exhibit the best view through the prominent dust lanes evident at optical wavelengths and provide a more accurate correction for the internal extinction. The relative fluxes of the emission lines we have detected in the blue together with those detected in the wavelength range 6295 - 6867 A by Bower et al. indicate that the gas at the nucleus is photoionized by a nonstellar process, instead of hot stars. Stellar absorption features from cool stars at the nucleus are very weak. We update the spectral energy distribution of the nuclear point source and find that although it is roughly flat in most bands, the optical to UV continuum is very red, similar to the spectral energy distribution of BL Lac. Thus, the nuclear point source seen in high-resolution optical images is not a star cluster but is instead a nonstellar source. Assuming isotropic emission from this source, we estimate that the ratio of bolometric luminosity to Eddington luminosity is about 5 x 10(exp -7). However, this could be underestimated if this source is a misaligned BL Lac object, which is a possibility suggested by the spectral energy distribution and the evidence of optical variability we describe.

[Effect of genotype and day or night time of testing on mice behavior in the light-dark box and the open-field tests].

PubMed

Morozova, M V; Kulikov, A V

2010-01-01

The light-dark box (LDB) and the open-field (OF) tests are widespread experimental models for studying locomotion and anxiety in laboratory rats and mice. The fact that rodents are nocturnal animals and more active at night raises a critical question of whether behavioral experiments carried out in the light phase are methodologically correct. Parameters of behavior of four mouse strains (C57BL/6J, DBA2/J, AKR/J and CBA/LacJ) in the light-dark box and open-field tests in the light and dark phases were compared. No significant influence of the phase of testing on anxiety in LDB and OF tests was revealed. In the OF test CBA mice showed increased locomotor activity, whereas AKR and C57BL/6 mice showed increased defecation in the dark phase. It was concluded that: 1) the phase of testing is not crucial for the expression of anxiety in LDB and OF; 2) the sensitivity to the phase of testing depends on the genotype; 3) the indices of behavior in the genotypes sensitive to the phase of testing (locomotion in the CBA and defecation in the AKR and C57BL/6 mouse strains) are increased in the dark phase.

The Blazar 3C 66A in 2003-2004: hadronic versus leptonic model fits

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reimer, A.; Joshi, M.; Boettcher, M.

2008-12-24

The low-frequency peaked BL Lac object 3C 66A was the subject of an extensive multi-wavelength campaign from July 2003 till April 2004, which included quasi-simultaneous observations at optical, X-rays and very high energy gamma-rays. Here we apply the hadronic Synchrotron-Proton Blazar (SPB) model to the observed spectral energy distribution time-averaged over a flaring state, and compare the resulting model fits to those obtained from the application of the leptonic Synchrotron-Self-Compton (SSC) model. The results are used to identify diagnostic key predictions of the two blazar models for future multi-wavelength observations.

BLAZAR SPECTRAL PROPERTIES AT 74 MHz

DOE Office of Scientific and Technical Information (OSTI.GOV)

Massaro, F.; Funk, S.; Giroletti, M.

2013-10-01

Blazars are the most extreme class of active galactic nuclei. Despite a previous investigation at 102 MHz for a small sample of BL Lac objects and our recent analysis of blazars detected in the Westerbork Northern Sky Survey, a systematic study of the blazar spectral properties at frequencies below 100 MHz has been never carried out. In this paper, we present the first analysis of the radio spectral behavior of blazars based on the recent Very Large Array Low-frequency Sky Survey (VLSS) at 74 MHz. We search for blazar counterparts in the VLSS catalog, confirming that they are detected atmore » 74 MHz. We then show that blazars present radio-flat spectra (i.e., radio spectral indices of ∼0.5) when evaluated, which also about an order of magnitude in frequency lower than previous analyses. Finally, we discuss the implications of our findings in the context of the blazars-radio galaxies connection since the low-frequency radio data provide a new diagnostic tool to verify the expectations of the unification scenario for radio-loud active galaxies.« less

EX56a study of extended X-ray emission around isolated galaxies EX56b identification and spectra of bright X-ray sources at high galactic latitude

NASA Technical Reports Server (NTRS)

Schwartz, Daniel A.

1987-01-01

The EXOSAT observations confirmed the identification and extended nature of PKS 2345-35. It gave a good 2 to 10 keV X-ray spectrum and a detailed spatial profile indicating asymmetry of the structure. In the high galactic latitidue investigation, the BL Lac object identified with the HEAO-1 source 1430+423 was detected, and the first X-ray spectrum was obtained. Several simulataneous observations of H0323+022 were obtained over a broad range of electromagnetic spectrum. Studies of luminous active galactic nuclei have given significant information on the spectrum of the quasar PKS 0558-504. In a study of Southern sky cataclysmic variables, the EXOSAT was used to determine the X-ray spectrum and search for periodicities in two objects. Studies of complete identifications have revealed that X-ray sources in two high galactic latitude fields are stars, and therefore are to be excluded from the Piccinotti extragalactic sample. Only one Piccinotti source remains to be identified.

Next-generation sequencing and clinical outcomes of patients with lung adenocarcinoma treated with stereotactic body radiotherapy.

PubMed

Cassidy, Richard J; Zhang, Xinyan; Patel, Pretesh R; Shelton, Joseph W; Escott, Chase E; Sica, Gabriel L; Rossi, Michael R; Hill, Charles E; Steuer, Conor E; Pillai, Rathi N; Ramalingam, Suresh S; Owonikoko, Taofeek K; Behera, Madhusmita; Force, Seth D; Fernandez, Felix G; Curran, Walter J; Higgins, Kristin A

2017-10-01

Genetic aberrations are well characterized in lung adenocarcinomas (LACs) and clinical outcomes have been influenced by targeted therapies in the advanced setting. Stereotactic body radiotherapy (SBRT) is the standard-of-care therapy for patients with nonoperable, early-stage LAC, but to the authors' knowledge, no information is available regarding the impact of genomic changes in these patients. The current study sought to determine the frequency and clinical impact of genetic aberrations in this population. Under an Institutional Review Board-approved protocol, the records of 242 consecutive patients with early-stage lung cancers were reviewed; inclusion criteria included LAC histology with an adequate tumor sample for the successful use of next-generation sequencing and fluorescence in situ hybridization testing. Univariate analysis was performed to identify factors associated with clinical outcomes. LAC samples from 98 of the 242 patients were reviewed (40.5%), of whom 45 patients (46.0%) had genetic testing. The following mutations were noted: KRAS in 20.0% of samples, BRAF in 2.2% of samples, SMAD family member 4 (SMAD4) in 4.4% of samples, epidermal growth factor receptor (EGFR) in 15.6% of samples, STK1 in 2.2% of samples, tumor protein 53 (TP53) in 15.6% of samples, and phosphatase and tensin homolog (PTEN) in 2.2% of samples. The following gene rearrangements were observed: anaplastic lymphoma kinase (ALK) in 8.9% of samples, RET in 2.2% of samples, and MET amplification in 17.8% of samples. The median total delivered SBRT dose was 50 grays (range, 48-60 grays) over a median of 5 fractions (range, 3-8 fractions). The KRAS mutation was associated with worse local control (odds ratio [OR], 3.64; P<.05). MET amplification was associated with worse regional (OR, 4.64; P<.05) and distant (OR, 3.73; P<.05) disease control. To the authors' knowledge, the current series is the first to quantify genetic mutations and their association with clinical outcomes in patients with early-stage LAC treated with SBRT. KRAS mutations were associated with worse local control and MET amplification was associated with worse regional and distant disease control, findings that need to be validated in a prospective setting. Cancer 2017;123:3681-3690. © 2017 American Cancer Society. © 2017 American Cancer Society.

Characterisation and discrimination of various types of lac resin using gas chromatography mass spectrometry techniques with quaternary ammonium reagents.

PubMed

Sutherland, K; del Río, J C

2014-04-18

A variety of lac resin samples obtained from artists' suppliers, industrial manufacturers, and museum collections were analysed using gas chromatography mass spectrometry (GCMS) and reactive pyrolysis GCMS with quaternary ammonium reagents. These techniques allowed a detailed chemical characterisation of microgram-sized samples, based on the detection and identification of derivatives of the hydroxy aliphatic and cyclic (sesquiterpene) acids that compose the resin. Differences in composition could be related to the nature of the resin, e.g. wax-containing (unrefined), bleached, or aged samples. Furthermore, differences in the relative abundances of aliphatic hydroxyacids appear to be associated with the biological source of the resin. The diagnostic value of newly characterised lac components, including 8-hydroxyacids, is discussed here for the first time. Identification of derivatised components was aided by AMDIS deconvolution software, and discrimination of samples was enhanced by statistical evaluation of data using principal component analysis. The robustness of the analyses, together with the minimal sample size required, make these very powerful approaches for the characterisation of lac resin in museum objects. The value of such analyses for enhancing the understanding of museum collections is illustrated by two case studies of objects in the collection of the Philadelphia Museum of Art: a restorer's varnish on a painting by Luca Signorelli, and a pictorial inlay in an early nineteenth-century High Chest by George Dyer. Copyright © 2014 Elsevier B.V. All rights reserved.

The host galaxy/AGN connection in nearby early-type galaxies. Is there a miniature radio-galaxy in every "core" galaxy?

NASA Astrophysics Data System (ADS)

Balmaverde, B.; Capetti, A.

2006-02-01

This is the second of a series of three papers exploring the connection between the multiwavelength properties of AGN in nearby early-type galaxies and the characteristics of their hosts. We selected two samples with 5 GHz VLA radio flux measurements down to 1 mJy, reaching levels of radio luminosity as low as 1036 erg s-1. In Paper I we presented a study of the surface brightness profiles for the 65 objects with available archival HST images out of the 116 radio-detected galaxies. We classified early-type galaxies into "core" and "power-law" galaxies, discriminating on the basis of the slope of their nuclear brightness profiles, following the Nukers scheme. Here we focus on the 29 core galaxies (hereafter CoreG). We used HST and Chandra data to isolate their optical and X-ray nuclear emission. The CoreG invariably host radio-loud nuclei, with an average radio-loudness parameter of Log R = L5 {GHz} / LB ˜ 3.6. The optical and X-ray nuclear luminosities correlate with the radio-core power, smoothly extending the analogous correlations already found for low luminosity radio-galaxies (LLRG) toward even lower power, by a factor of ˜ 1000, covering a combined range of 6 orders of magnitude. This supports the interpretation of a common non-thermal origin of the nuclear emission also for CoreG. The luminosities of the nuclear sources, most likely dominated by jet emission, set firm upper limits, as low as L/L_Edd ˜ 10-9 in both the optical and X-ray band, on any emission from the accretion process. The similarity of CoreG and LLRG when considering the distributions host galaxies luminosities and black hole masses, as well as of the surface brightness profiles, indicates that they are drawn from the same population of early-type galaxies. LLRG represent only the tip of the iceberg associated with (relatively) high activity levels, with CoreG forming the bulk of the population. We do not find any relationship between radio-power and black hole mass. A minimum black hole mass of M_BH = 108 M⊙ is apparently associated with the radio-loud nuclei in both CoreG and LLRG, but this effect must be tested on a sample of less luminous galaxies, likely to host smaller black holes. In the unifying model for BL Lacs and radio-galaxies, CoreG likely represent the counterparts of the large population of low luminosity BL Lac now emerging from the surveys at low radio flux limits. This suggests the presence of relativistic jets also in these quasi-quiescent early-type "core" galaxies.

The Ringo2 Optical Polarisation Catalogue of 13 High-Energy Blazars

NASA Astrophysics Data System (ADS)

Barres de Almeida, Ulisses; Jermak, Helen; Mundell, Carole; Lindfors, Elina; Nilsson, Kari; Steele, Iain

2015-08-01

We present the findings of the Ringo2 3-year survey of 13 blazars (3 FSRQs and 10 BL Lacs) with regular coverage and reasonably fast cadence of one to three observations a week. Ringo2 was installed on the Liverpool Robotic Telescope (LT) on the Canary Island of La Palma between 2009 and 2012 and monitored thirteen high-energy-emitting blazars in the northern sky. The objects selected as well as the observational strategy were tuned to maximise the synergies with high-energy X- to gamma-ray observations. Therefore this sample stands out as a well-sampled, long-term view of high-energy AGN jets in polarised optical light. Over half of the sources exhibited an increase in optical flux during this period and almost a quarter were observed in outburst. We compare the optical data to gamma (Fermi/LAT) and X-ray data during these periods of outburst. In this talk we present the data obtained for all sources over the lifetime of Ringo2 with additional optical data from the KVA telescope and the SkyCamZ wide-field camera (on the LT), we explore the relationship between the change in polarisation angle as a function of time (dEVPA/dMJD), flux and polarisation degree along with cross correlation comparisons of optical and high-energy flux.

"Phloem sap analysis of Schleichera oleosa (Lour) Oken, Butea monosperma (Lam) Taub. and Ziziphus mauritiana (Lam) and hemolymph of Kerria lacca (Kerr) using HPLC and tandem mass spectrometry".

PubMed

Vashishtha, Amit; Rathi, Brijesh; Kaushik, Sandeep; Sharma, K K; Lakhanpaul, Suman

2013-10-01

Females of lac insects especially of Kerria lacca (Kerr) secret a resin known as lac for their own protection, which has tremendous applications. Lac insect completes its lifecycle on several host taxa where it exclusively feeds on phloem sap but Schleichera oleosa (Lour.) Oken, Butea monosperma (Lam.) and Ziziphus mauritiana (Lam.) are its major hosts. Analysis of phloem sap constituents as well as hemolymph of lac insect is important because it ultimately gets converted into lac by insect intervention. Main phloem sap constituent's viz. sugars and free amino acids and hemolymph of lac insect were analyzed using HPLC and tandem mass spectrometry, respectively. The results were transformed to relative percentage of the total sugars and free amino acids analyzed in each sample for comparison among lac insect hemolymph and the phloem sap of the three different host taxa. Sucrose (58.9 ± 3.6-85.6 ± 0.9) and trehalose (62.3 ± 0.4) were the predominant sugars in phloem sap of three taxa and hemolymph of lac insect, respectively. Glutamic acid (33.1 ± 1.4-39.8 ± 1.4) was found to be main amino acid among the phloem sap of three taxa while tyrosine (61 ± 2.6) was the major amino acid in hemolymph of lac insect. The relative percentage of non-essential amino acids (60.8 %-69.9 %) was found to be more in all the three host taxa while essential amino acids (30.1 %-35.4 %) were present at a lower relative percentage. In contrast to this, the relative percentage of essential amino acids (81.9 %) was observed to be higher as compared to non-essential amino acids (17.7 %) in lac insect hemolymph. These results led to the detection of lac insect's endosymbionts. Moreover, this study revealed a clue regarding the importance of development of a synthetic diet for this insect so that a precise pathway of lac biosynthesis could be investigated for thorough understanding.

Tetherin promotes the innate and adaptive cell-mediated immune response against retrovirus infection in vivo.

PubMed

Li, Sam X; Barrett, Bradley S; Heilman, Karl J; Messer, Ronald J; Liberatore, Rachel A; Bieniasz, Paul D; Kassiotis, George; Hasenkrug, Kim J; Santiago, Mario L

2014-07-01

Tetherin/BST-2 is a host restriction factor that could directly inhibit retroviral particle release by tethering nascent virions to the plasma membrane. However, the immunological impact of Tetherin during retrovirus infection remains unknown. We now show that Tetherin influences antiretroviral cell-mediated immune responses. In contrast to the direct antiviral effects of Tetherin, which are dependent on cell surface expression, the immunomodulatory effects are linked to the endocytosis of the molecule. Mice encoding endocytosis-competent C57BL/6 Tetherin exhibited lower viremia and pathology at 7 d postinfection with Friend retrovirus (FV) compared with mice encoding endocytosis-defective NZW/LacJ Tetherin. Notably, antiretroviral protection correlated with stronger NK cell responses. In addition, Friend retrovirus infection levels were significantly lower in wild-type C57BL/6 mice than in Tetherin knockout mice at 2 wk postinfection, and antiretroviral protection correlated with stronger NK cell and virus-specific CD8+ T cell responses. The results demonstrate that Tetherin acts as a modulator of the cell-mediated immune response against retrovirus infection in vivo.

Fourier analysis of blazar variability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Finke, Justin D.; Becker, Peter A., E-mail: justin.finke@nrl.navy.mil

Blazars display strong variability on multiple timescales and in multiple radiation bands. Their variability is often characterized by power spectral densities (PSDs) and time lags plotted as functions of the Fourier frequency. We develop a new theoretical model based on the analysis of the electron transport (continuity) equation, carried out in the Fourier domain. The continuity equation includes electron cooling and escape, and a derivation of the emission properties includes light travel time effects associated with a radiating blob in a relativistic jet. The model successfully reproduces the general shapes of the observed PSDs and predicts specific PSD and timemore » lag behaviors associated with variability in the synchrotron, synchrotron self-Compton, and external Compton emission components, from submillimeter to γ-rays. We discuss applications to BL Lacertae objects and to flat-spectrum radio quasars (FSRQs), where there are hints that some of the predicted features have already been observed. We also find that FSRQs should have steeper γ-ray PSD power-law indices than BL Lac objects at Fourier frequencies ≲ 10{sup –4} Hz, in qualitative agreement with previously reported observations by the Fermi Large Area Telescope.« less

THE OPTICAL MICROVARIABILITY AND SPECTRAL CHANGES OF THE BL LACERTAE OBJECT S5 0716+714

DOE Office of Scientific and Technical Information (OSTI.GOV)

Poon, H.; Fu, J. N.; Fan, J. H.

We monitored the BL Lac object S5 0716+714 in the optical band during 2008 October and December and 2009 February with a best temporal resolution of about 5 minutes in the BVRI bands. Four fast flares were observed with amplitudes ranging from 0.3 to 0.75 mag. The source remained active during the whole monitoring campaign, showing microvariability in all days except for one. The overall variability amplitudes are {delta}B {approx} 0fm89, {delta}V {approx} 0fm80, {delta}R {approx} 0fm73, and {delta}I{approx} 0fm51. Typical timescales of microvariability range from 2 to 8 hr. The overall V - R color index ranges from 0.37more » to 0.59. Strong bluer-when-brighter chromatism was found on internight timescales. However, a different spectral behavior was found on intranight timescales. A possible time lag of {approx}11 minutes between B and I bands was found on one night. The shock-in-jet model and geometric effects can be applied to explain the source's intranight behavior.« less

Optical Spectra of Candidate International Celestial Reference Frame (ICRF) Flat-spectrum Radio Sources. III.

NASA Astrophysics Data System (ADS)

Titov, O.; Pursimo, T.; Johnston, Helen M.; Stanford, Laura M.; Hunstead, Richard W.; Jauncey, David L.; Zenere, Katrina A.

2017-04-01

In extending our spectroscopic program, which targets sources drawn from the International Celestial Reference Frame (ICRF) Catalog, we have obtained spectra for ˜160 compact, flat-spectrum radio sources and determined redshifts for 112 quasars and radio galaxies. A further 14 sources with featureless spectra have been classified as BL Lac objects. Spectra were obtained at three telescopes: the 3.58 m European Southern Observatory New Technology Telescope, and the two 8.2 m Gemini telescopes in Hawaii and Chile. While most of the sources are powerful quasars, a significant fraction of radio galaxies is also included from the list of non-defining ICRF radio sources.

Optical Spectra of Candidate International Celestial Reference Frame (ICRF) Flat-spectrum Radio Sources. III

DOE Office of Scientific and Technical Information (OSTI.GOV)

Titov, O.; Stanford, Laura M.; Pursimo, T.

In extending our spectroscopic program, which targets sources drawn from the International Celestial Reference Frame (ICRF) Catalog, we have obtained spectra for ∼160 compact, flat-spectrum radio sources and determined redshifts for 112 quasars and radio galaxies. A further 14 sources with featureless spectra have been classified as BL Lac objects. Spectra were obtained at three telescopes: the 3.58 m European Southern Observatory New Technology Telescope, and the two 8.2 m Gemini telescopes in Hawaii and Chile. While most of the sources are powerful quasars, a significant fraction of radio galaxies is also included from the list of non-defining ICRF radiomore » sources.« less

Multiwavelength studies of the blazars detected by AGILE

NASA Astrophysics Data System (ADS)

Filippo D'Ammando

2010-02-01

The discovery of emission in the gamma-ray domain from many Active Galactic Nuclei (AGNs) by EGRET on board the Compton Gamma-Ray Observatory and the Cherenkov Telescopes was one of the most breakthrough of high energy astrophysics in the last 20 years, leading to the identification of a new class of AGNs: the blazars. Blazars are the most extreme subclass of AGNs, characterized by the emission of strong non-thermal radiation across the entire electromagnetic spectrum, from radio to very high gamma-ray energies. This emission is interpreted as the result of the electromagnetic radiation from a relativistic jet that is viewed closely aligned to the line of sight of the observer, thus causing strong relativistic amplification. Considering that the large fraction of the total power of blazars is emitted in the gamma-rays, information in this energy band is crucial to study the different radiation models. More than ten years after the EGRET era, the AGILE satellite (and subsequently also the Fermi satellite) filled the gap in the MeV-GeV band giving further impulse to the study of the high-energy astrophysics phenomena in blazars. However, notwithstanding the importance of the information provided by the gamma-ray observations, correlated multiwavelength studies are the key to achieve a better understanding of the structure of the inner jet, the origin of the seed photons for the inverse Compton process and the emission mechanisms at work in blazars. Since its launch in April 2007, the AGILE satellite detected several blazars in high activity state: PKS 1510-089, S5 0716+714, 3C 454.3, 3C 273, 3C 279, W Comae, Mrk 421 and PG 1553+113. In this Thesis I will present the most interesting results on multifrequency analysis of these sources detected by AGILE in gamma-rays, together with the multiwavelength data from other observatories such as Spitzer, Swift, RXTE, Suzaku, INTEGRAL, MAGIC, VERITAS, as well as radio-to-optical coverage by means of GASP-WEBT and REM. This large multifrequency coverage gave me the opportunity to study the Spectral Energy Distributions (SEDs) of these sources from radio to gamma-rays, the correlated variability in different energy bands and to investigate the mechanisms responsible for their emission, uncovering in some cases a more complex behaviour with respect to the standard models. The intense gamma-ray flares of S5 0716+714 observed by AGILE in September and October 2007 are among the highest fluxes detected by a BL Lac object and considering the redshift of the source (z = 0.31) the total power transported in the jet during these episodes approaches or slightly exceeds the maximum power generated by a spinning black hole of 10^9 solar masses, challenging the Blandford-Znajek mechanism and confirming the extreme energetics during these flares. The modeling of the SEDs of S5 0716+714 indicated as, even if the broad band emission appears in agreement with the synchrotron self Compton (SSC) paradigm, a more complex model with two SSC components is needed to interpret our data. The case of S5 0716+714 is not unique among the BL Lac objects, also for the multifrequency observation of Mrk 421 and W Comae in June 2008 a one-zone SSC model seems to be a good representation of the broad band spectrum, but the observations collected during the multiwavelength campaigns seem to open to more complex interpretations of the data. Moreover, the dominant emission mechanism in the gamma-ray band for Flat Spectrum Radio Quasars (FSRQs) is the inverse Compton scattering of external photons from the broad line region, but in some particular states also the contribution of seed photons from a hot corona (3C 454.3 in December 2007) or the accretion disk (3C 279) are shown to be important. Therefore, from the modeling of the different SEDs of BL Lacs and FSRQs observed by AGILE seems to emerge that the SSC and the external Compton (EC) frameworks, respectively, are good approximation for describing on average the high activity states of the two flavours of blazars, but going into details of the single observation more complex scenarios sometimes are requested. The possibility to obtain information over the entire electromagnetic spectrum during the multifrequency campaigns organized by AGILE gave me also the opportunity to investigate in some blazars the presence of Seyfert-like features, such as the little and big blue bumps (PKS 1510-089) and the Compton reflection component (3C 273). Moreover, we revealed in the FSRQ PKS 1510-089 some features typical of High-frequency peaked BL Lac objects, such a X-ray harder-when-brighter behaviour during March 2008 and a shift of the synchrotron peak towards higher frequencies during the huge flare of March 2009. Emission in optical and gamma-ray bands seems to be correlated during high activity states of blazars, but not strongly, with a possible lag of the gamma-ray flux with respect to optical one less than one day, both for FSRQs (e.g. 3C 454.3) and BL Lacs (S5 0716+714). On the other hand, during March 2009 a possible delay of the optical emission with respect to the gamma-ray one is detected for PKS 1510-089, suggesting a more complex behaviour in the optical/gamma-ray correlation, especially for FSRQs, where also a contribution of the thermal disk emission is clearly visible.

LUNSORT list of lunar orbiter data by LAC area

NASA Technical Reports Server (NTRS)

Hixon, S.

1976-01-01

Lunar orbiter (missions 1-5) photographic data are listed sequentially according to the number (1 to 147) LAC (Lunar Aeronautical Chart) areas by use of a computer program called LUNSORT. This listing, as well as a similar one from Apollo would simplify the task of identifying images of a given Lunar area. Instructions and sample case are included.

Microbial keratitis in los angeles: the doheny eye institute and the los angeles county hospital experience.

PubMed

Sand, Daniel; She, Rosemary; Shulman, Ira A; Chen, David S; Schur, Mathew; Hsu, Hugo Y

2015-05-01

To evaluate the spectrum and antibiotic susceptibility panel of infectious keratitis at a major tertiary care referral eye center and a major county hospital in Southern California. Retrospective case series. All cultured infectious keratitis cases from July 1, 2008, through December 31, 2012, from the Doheny Eye Institute (DEI) and the Los Angeles County + University of Southern California Medical Center (LAC+USC) were evaluated. Microbiology records were reviewed retrospectively. Microbial isolates as well as antibiotic susceptibility patterns were analyzed. One hundred eighty-four (63%) of 290 cases showed positive culture results at DEI and 152 (82%) of 186 cases showed positive culture results at LAC+USC. Gram-positive pathogens were found to be the most common at both DEI (70%) and LAC+USC (68%), with coagulase-negative Staphylococcus being the most common gram-positive organism (58% at DEI and 44% at LAC+USC). Pseudomonas aeruginosa was the most common gram-negative organism (57% at DEI and 43% at LAC+USC). Ciprofloxacin and levofloxacin susceptibility for all tested pathogens was 73% at DEI and 81% at LAC+USC (P = 0.16). Oxacillin-resistant Staphylococcus aureus (ORSA) was found in 42% of cases at DEI and in 45% of cases at LAC+USC (P = 1.00). There is no significant difference in the spectrum of pathogens or antibiotic susceptibility of pathogens at DEI versus LAC+USC, and ORSA was found in approximately half of all S. aureus samples. Copyright © 2015 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

Effect of the inclusion of chestnut in the finishing diet on volatile compounds during the manufacture of dry-cured "Lacón" from Celta pig breed.

PubMed

Lorenzo, José M; Franco, Daniel; Carballo, Javier

2014-01-01

The effect of the finishing diet on the volatile compounds throughout the manufacture of dry-cured "lacón" (a Spanish traditional meat product), from the Celta pig breed was studied. Thirty-six pigs were separated into three groups according to the type of feeding during the finish-fattening period of three months (concentrate, mixed diet and chestnut). From the pigs of each diet, four batches of dry-cured "lacón" were manufactured. From each batch, samples of fresh meat, meat after salting, after post-salting, and after 14, 28, 56 and 84 days of drying-ripening were taken. Volatiles were extracted by a purge-and-trap method and analyzed by gas chromatographic/mass spectrometry (GC/MS). Seventy-six volatile compounds were identified and quantified from dry-cured "lacón" samples in pigs finished with chestnut, eighty-two for concentrate fed pigs and eighty in pigs fed with the mixed diet. The number of identified volatile compounds increased during the manufacturing process; at 84 days of drying-ripening, in the dry-cured "lacón" samples from pigs finished with concentrate, mixed diet and chestnut, 54, 58 and 62 volatile compounds were detected, respectively. The most abundant group of flavour compounds at the end of the manufacturing process was hydrocarbons in the three feeding systems, followed by aldehydes, ketones and alcohols. Discriminant analysis selected six variables (dodecane, butadienol, pentenol, 2-pentenal, decen-3-ona and pyridine-2-methyl) and calculated two discriminating functions which allowed verification of chestnut in the finishing diet. © 2013.

Science with the ASTRI mini-array for the Cherenkov Telescope Array: blazars and fundamental physics

NASA Astrophysics Data System (ADS)

Bonnoli, Giacomo; Tavecchio, Fabrizio; Giuliani, Andrea; Bigongiari, Ciro; Di Pierro, Federico; Stamerra, Antonio; Pareschi, Giovanni; Vercellone, Stefano; ASTRI Collaboration; CTA Consortium

2016-05-01

ASTRI (“Astronomia a Specchi con Tecnologia Replicante Italiana”) is a flagship project of the Italian Ministry of Research (MIUR), devoted to the realization, operation and scientific validation of an end-to-end prototype for the Small Size Telescope (SST) envisaged to become part of the Cherenkov Telescope Array (CTA). The ASTRI SST-2M telescope prototype is characterized by a dual mirror, Schwarzschild-Couder optical design and a compact camera based on silicon photo-multipliers. It will be sensitive to multi-TeV very high energy (VHE) gamma rays up to 100 TeV, with a PSF ~ 6’ and a wide (9.6°) unaberrated optical field of view. Right after validation of the design in single-dish observations at the Serra La Nave site (Sicily, Italy) during 2015, the ASTRI collaboration will be able to start deployment, at the final CTA southern site, of the ASTRI mini-array, proposed to constitute the very first CTA precursor. Counting 9 ASTRI SST-2M telescopes, the ASTRI mini-array will overtake current IACT systems in differential sensitivity above 5 TeV, thus allowing unprecedented observations of known and predicted bright TeV emitters in this band, including some extragalactic sources such as extreme high-peaked BL Lacs with hard spectra. We exploited the ASTRI scientific simulator ASTRIsim in order to understand the feasibility of observations tackling blazar and cosmic ray physics, including discrimination of hadronic and leptonic scenarios for the VHE emission from BL Lac relativistic jets and indirect measurements of the intergalactic magnetic field and of the extragalactic background light. We selected favorable targets, outlining observation modes, exposure times, multi-wavelength coverage needed and the results expected. Moreover, the perspectives for observation of effects due to the existence of axion-like particles or to Lorentz invariance violations have been investigated.

MAGIC detection of short-term variability of the high-peaked BL Lac object 1ES 0806+524

DOE PAGES

Aleksi , J.; Ansoldi, S.; Antonelli, L. A.; ...

2015-05-29

The high-frequency-peaked BL Lac (HBL) 1ES 0806+524 (z = 0.138) was discovered in very high energy (VHE) γ-rays in 2008. Until now, the broad-band spectrum of 1ES 0806+524 has been only poorly characterized, in particular at high energies. In this paper, we analysed multiwavelength observations from γ-rays to radio performed from 2011 January to March, which were triggered by the high activity detected at optical frequencies. These observations constitute the most precise determination of the broad-band emission of 1ES 0806+524 to date. The stereoscopic Major Atmospheric Gamma-Ray Imaging Cherenkov (MAGIC) observations yielded a γ-ray signal above 250 GeV of (3.7more » ± 0.7) per cent of the Crab Nebula flux with a statistical significance of 9.9σ. The multiwavelength observations showed significant variability in essentially all energy bands, including a VHE γ-ray flare that lasted less than one night, which provided unprecedented evidence for short-term variability in 1ES 0806+524. The spectrum of this flare is well described by a power law with a photon index of 2.97 ± 0.29 between ~150 GeV and 1 TeV and an integral flux of (9.3 ± 1.9) per cent of the Crab nebula flux above 250 GeV. The spectrum during the non-flaring VHE activity is compatible with the only available VHE observation performed in 2008 with VERITAS when the source was in a low optical state. Lastly, the broad-band spectral energy distribution can be described with a one-zone synchrotron self-Compton model with parameters typical for HBLs, indicating that 1ES 0806+524 is not substantially different from the HBLs previously detected.« less

PKS 2005-489 at VHE: four years of monitoring with HESS and simultaneous multi-wavelength observations

NASA Astrophysics Data System (ADS)

H.E.S.S. Collaboration; Acero, F.; Aharonian, F.; Akhperjanian, A. G.; Anton, G.; Barres de Almeida, U.; Bazer-Bachi, A. R.; Becherini, Y.; Behera, B.; Benbow, W.; Bernlöhr, K.; Bochow, A.; Boisson, C.; Bolmont, J.; Borrel, V.; Brucker, J.; Brun, F.; Brun, P.; Bühler, R.; Bulik, T.; Büsching, I.; Boutelier, T.; Chadwick, P. M.; Charbonnier, A.; Chaves, R. C. G.; Cheesebrough, A.; Chounet, L.-M.; Clapson, A. C.; Coignet, G.; Costamante, L.; Dalton, M.; Daniel, M. K.; Davids, I. D.; Degrange, B.; Deil, C.; Dickinson, H. J.; Djannati-Ataï, A.; Domainko, W.; O'C. Drury, L.; Dubois, F.; Dubus, G.; Dyks, J.; Dyrda, M.; Egberts, K.; Eger, P.; Espigat, P.; Fallon, L.; Farnier, C.; Fegan, S.; Feinstein, F.; Fiasson, A.; Förster, A.; Fontaine, G.; Füßling, M.; Gabici, S.; Gallant, Y. A.; Gérard, L.; Gerbig, D.; Giebels, B.; Glicenstein, J. F.; Glück, B.; Goret, P.; Göring, D.; Hauser, M.; Heinz, S.; Heinzelmann, G.; Henri, G.; Hermann, G.; Hinton, J. A.; Hoffmann, A.; Hofmann, W.; Hofverberg, P.; Holleran, M.; Hoppe, S.; Horns, D.; Jacholkowska, A.; de Jager, O. C.; Jahn, C.; Jung, I.; Katarzyński, K.; Katz, U.; Kaufmann, S.; Kerschhaggl, M.; Khangulyan, D.; Khélifi, B.; Keogh, D.; Klochkov, D.; Kluźniak, W.; Kneiske, T.; Komin, Nu.; Kosack, K.; Kossakowski, R.; Lamanna, G.; Lenain, J.-P.; Lohse, T.; Marandon, V.; Martineau-Huynh, O.; Marcowith, A.; Masbou, J.; Maurin, D.; McComb, T. J. L.; Medina, M. C.; Méhault, J.; Moderski, R.; Moulin, E.; Naumann-Godo, M.; de Naurois, M.; Nedbal, D.; Nekrassov, D.; Nicholas, B.; Niemiec, J.; Nolan, S. J.; Ohm, S.; Olive, J.-F.; de Oña Wilhelmi, E.; Orford, K. J.; Ostrowski, M.; Panter, M.; Paz Arribas, M.; Pedaletti, G.; Pelletier, G.; Petrucci, P.-O.; Pita, S.; Pühlhofer, G.; Punch, M.; Quirrenbach, A.; Raubenheimer, B. C.; Raue, M.; Rayner, S. M.; Renaud, M.; Rieger, F.; Ripken, J.; Rob, L.; Rosier-Lees, S.; Rowell, G.; Rudak, B.; Rulten, C. B.; Ruppel, J.; Sahakian, V.; Santangelo, A.; Schlickeiser, R.; Schöck, F. M.; Schwanke, U.; Schwarzburg, S.; Schwemmer, S.; Shalchi, A.; Sikora, M.; Skilton, J. L.; Sol, H.; Stawarz, Ł.; Steenkamp, R.; Stegmann, C.; Stinzing, F.; Superina, G.; Szostek, A.; Tam, P. H.; Tavernet, J.-P.; Terrier, R.; Tibolla, O.; Tluczykont, M.; van Eldik, C.; Vasileiadis, G.; Venter, C.; Venter, L.; Vialle, J. P.; Vincent, P.; Vivier, M.; Völk, H. J.; Volpe, F.; Wagner, S. J.; Ward, M.; Zdziarski, A. A.; Zech, A.

2010-02-01

Aims: Our aim is to study the very high energy (VHE; E>100 GeV) γ-ray emission from BL Lac objects and the evolution in time of their broad-band spectral energy distribution (SED). Methods: VHE observations of the high-frequency peaked BL Lac object PKS 2005-489 were made with the High Energy Stereoscopic System (HESS) from 2004 through 2007. Three simultaneous multi-wavelength campaigns at lower energies were performed during the HESS data taking, consisting of several individual pointings with the XMM-Newton and RXTE satellites. Results: A strong VHE signal, ~17σ total, from PKS 2005-489 was detected during the four years of HESS observations (90.3 h live time). The integral flux above the average analysis threshold of 400 GeV is ~3% of the flux observed from the Crab Nebula and varies weakly on time scales from days to years. The average VHE spectrum measured from ~300 GeV to ~5 TeV is characterized by a power law with a photon index, Γ = 3.20± 0.16_stat± 0.10_syst. At X-ray energies the flux is observed to vary by more than an order of magnitude between 2004 and 2005. Strong changes in the X-ray spectrum (ΔΓX ≈ 0.7) are also observed, which appear to be mirrored in the VHE band. Conclusions: The SED of PKS 2005-489, constructed for the first time with contemporaneous data on both humps, shows significant evolution. The large flux variations in the X-ray band, coupled with weak or no variations in the VHE band and a similar spectral behavior, suggest the emergence of a new, separate, harder emission component in September 2005. Supported by CAPES Foundation, Ministry of Education of Brazil.Now at Harvard-Smithsonian Center for Astrophysics, Cambridge, USA.Now at W.W. Hansen Experimental Physics Laboratory & Kavli Institute for Particle Astrophysics and Cosmology, Stanford University, Stanford, USA.

VERITAS Observations of the BL Lac Object PG 1553+113

NASA Astrophysics Data System (ADS)

Aliu, E.; Archer, A.; Aune, T.; Barnacka, A.; Behera, B.; Beilicke, M.; Benbow, W.; Berger, K.; Bird, R.; Buckley, J. H.; Bugaev, V.; Byrum, K.; Cardenzana, J. V.; Cerruti, M.; Chen, X.; Ciupik, L.; Connolly, M. P.; Cui, W.; Dickinson, H. J.; Dumm, J.; Eisch, J. D.; Errando, M.; Falcone, A.; Federici, S.; Feng, Q.; Finley, J. P.; Fortin, P.; Fortson, L.; Furniss, A.; Galante, N.; Gillanders, G. H.; Griffin, S.; Griffiths, S. T.; Grube, J.; Gyuk, G.; Håkansson, N.; Hanna, D.; Holder, J.; Hughes, G.; Humensky, T. B.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kertzman, M.; Khassen, Y.; Kieda, D.; Krawczynski, H.; Krennrich, F.; Kumar, S.; Lang, M. J.; Madhavan, A.; McArthur, S.; McCann, A.; Meagher, K.; Millis, J.; Moriarty, P.; Nieto, D.; O'Faoláin de Bhróithe, A.; Ong, R. A.; Orr, M.; Otte, A. N.; Park, N.; Perkins, J. S.; Pohl, M.; Popkow, A.; Prokoph, H.; Pueschel, E.; Quinn, J.; Ragan, K.; Rajotte, J.; Reyes, L. C.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Sembroski, G. H.; Shahinyan, K.; Staszak, D.; Telezhinsky, I.; Tucci, J. V.; Tyler, J.; Varlotta, A.; Vassiliev, V. V.; Wakely, S. P.; Weinstein, A.; Welsing, R.; Wilhelm, A.; Williams, D. A.; Zitzer, B.

2015-01-01

We present results from VERITAS observations of the BL Lac object PG 1553+113 spanning the years 2010, 2011, and 2012. The time-averaged spectrum, measured between 160 and 560 GeV, is well described by a power law with a spectral index of 4.33 ± 0.09. The time-averaged integral flux above 200 GeV measured for this period was (1.69 ± 0.06) × 10-11 photons cm-2 s-1, corresponding to 6.9% of the Crab Nebula flux. We also present the combined γ-ray spectrum from the Fermi Large Area Telescope and VERITAS covering an energy range from 100 MeV to 560 GeV. The data are well fit by a power law with an exponential cutoff at 101.9 ± 3.2 GeV. The origin of the cutoff could be intrinsic to PG 1553+113 or be due to the γ-ray opacity of our universe through pair production off the extragalactic background light (EBL). Given lower limits to the redshift of z > 0.395 based on optical/UV observations of PG 1553+113, the cutoff would be dominated by EBL absorption. Conversely, the small statistical uncertainties of the VERITAS energy spectrum have allowed us to provide a robust upper limit on the redshift of PG 1553+113 of z <= 0.62. A strongly elevated mean flux of (2.50 ± 0.14) × 10-11 photons cm-2 s-1 (10.3% of the Crab Nebula flux) was observed during 2012, with the daily flux reaching as high as (4.44 +/- 0.71) × 10-11 {photons} {cm}-2 {s}-1 (18.3% of the Crab Nebula flux) on MJD 56048. The light curve measured during the 2012 observing season is marginally inconsistent with a steady flux, giving a χ2 probability for a steady flux of 0.03%.

Escherichia coli DNA contamination in AmpliTaq Gold polymerase interferes with TaqMan analysis of lacZ.

PubMed

Koponen, Jonna K; Turunen, Anna-Mari; Ylä-Herttuala, Seppo

2002-03-01

Real-time PCR is a powerful method for the quantification of gene expression in biological samples. This method uses TaqMan chemistry based on the 5' -exonuclease activity of the AmpliTaq Gold DNA polymerase which releases fluorescence from hybridized probes during synthesis of each new PCR product. Many gene therapy studies use lacZ, encoding Escherichia coli beta-galactosidase, as a marker gene. Our results demonstrate that E. coli DNA contamination in AmpliTaq Gold polymerase interferes with TaqMan analysis of lacZ gene expression and decreases sensitivity of the method below the level required for biodistribution and long-term gene expression studies. In biodistribution analyses the contamination can lead to false-negative results by masking low-level lacZ expression in target and ectopic tissues, and false-positive results if sufficient controls are not used. We conclude that, to get reliable TaqMan results with lacZ, adequate controls should be included in each run to rule out contamination from AmpliTaq Gold polymerase.

[Effect of Transcutaneous Acupoint Electrical Stimulation on Hemodynamic Fluctuation Caused by Loosing Tourniquet in Elderly Patients Undergoing Knee Joint Replacement].

PubMed

Liang, Han-Sheng; Feng, Yi

2017-12-25

To observe the effect of transcutaneous acupoint electrical stimulation (TAES) on hemodynamic fluctuation caused by loosing tourniquet in the elderly patients undergoing knee joint replacement. A total of 60 ASA (America Society Anesthesiologist) I or II elderly patients for elective knee joint replacement surgery were randomly divided into control group (30 cases) and TAES group (30 cases). Patients of both groups were treated by intravenous anesthesia, and monitored with bispectral index (BIS, between 45－60) for anesthesia depth, stroke volume variation (SVV) for fluid management, mean arterial pressure (MAP) and cardiac index (CI) for hemodynamic fluctuation evaluation, and with analgesia nociception index (ANI, between 50－70) for remifentanil dosage adjustment. TAES (2 Hz/100 Hz, 8－20 mA) was applied to bilateral Xinshu (BL 15), Feishu (BL 13), Neiguan (PC 6) and Hegu (LI 4) acupoints for 30 min first (followed by anesthesia induction and operation), and given continuously until 15 min after tourniquet loosing. Patients of the control group were only given with electrodes attachment without electrical stimulation. The levels of MAP, CI, and arterial blood pH, PaCO 2 , PaO 2 , base excess (BE) and lactic acid (Lac) 1 min before, and 5 and 15 min after tourniquet loosing, and the dosages of remifentanil and ephedrine after tourniquet loosing were recorded. The changed levels of MAP, CI and blood Lac at 5 min after tourniquet loosing (relevant to the baseline levels), and blood Lac content at 15 min after tourniquet loosing (relevant to 5 min after tourniquet loosing) were significantly lower in the TAES group than in the control group ( P <0.05), but the levels of MAP and CI at 15 min after tourniquet loosing (relevant to 5 min following tourniquet loosing) were significantly higher in the TAES group than in the control group ( P <0.05), suggesting an improvement of blood pressure, cardiac function and substance metabolism after TAES. Moreover, the dosages of remifentanil and ephedrine after tourniquet loosing were considerably lower in the TAES group than in the control group ( P <0.05), suggesting a better stabilization of hemodynamics after TAES. No significant differences were found between the two groups in the levels of blood pH, PaCO 2 , PaO 2 and BE at 5 and 15 min after tourniquet loosing ( P >0.05). TAES has a positive effect on hemodynamics fluctuation caused by loosing tourniquet in the aged patients undergoing knee joint replacement.

Initiation of a lightning search using the lightning and airglow camera onboard the Venus orbiter Akatsuki

NASA Astrophysics Data System (ADS)

Takahashi, Yukihiro; Sato, Mitsuteru; Imai, Masataka; Lorenz, Ralph; Yair, Yoav; Aplin, Karen; Fischer, Georg; Nakamura, Masato; Ishii, Nobuaki; Abe, Takumi; Satoh, Takehiko; Imamura, Takeshi; Hirose, Chikako; Suzuki, Makoto; Hashimoto, George L.; Hirata, Naru; Yamazaki, Atsushi; Sato, Takao M.; Yamada, Manabu; Murakami, Shin-ya; Yamamoto, Yukio; Fukuhara, Tetsuya; Ogohara, Kazunori; Ando, Hiroki; Sugiyama, Ko-ichiro; Kashimura, Hiroki; Ohtsuki, Shoko

2018-05-01

The existence of lightning discharges in the Venus atmosphere has been controversial for more than 30 years, with many positive and negative reports published. The lightning and airglow camera (LAC) onboard the Venus orbiter, Akatsuki, was designed to observe the light curve of possible flashes at a sufficiently high sampling rate to discriminate lightning from other sources and can thereby perform a more definitive search for optical emissions. Akatsuki arrived at Venus during December 2016, 5 years following its launch. The initial operations of LAC through November 2016 have included a progressive increase in the high voltage applied to the avalanche photodiode detector. LAC began lightning survey observations in December 2016. It was confirmed that the operational high voltage was achieved and that the triggering system functions correctly. LAC lightning search observations are planned to continue for several years.

Water quality and hydrology of the Lac Vieux Desert watershed, Gogebic County, Michigan, and Vilas County, Wisconsin, 2002-04

USGS Publications Warehouse

Weaver, T.L.; Neff, B.P.; Ellis, J.M.

2005-01-01

Lac Vieux Desert is a prominent 6.6 square-mile lake that straddles the Michigan-Wisconsin border and forms the headwaters of the Wisconsin River. For generations, the Lac Vieux Desert Band of Lake Superior Chippewa Indians have used Lac Vieux Desert and the surrounding area for growing and harvesting wild rice, and hunting and fishing. The Lac Vieux Desert Band is concerned about the impact of lake-stage regulation on hydrology and ecology, and the impact on water quality of development along and near the shore, and recreational watercraft use and sport fishing. In 2005, the U.S. Geological Survey completed a water-resources investigation of the Lac Vieux Desert watershed in cooperation with the Lac Vieux Desert Band of Lake Superior Chippewa Indians.Water quality of Lac Vieux Desert is typical of many lakes in the northern United States. Trophic State Index calculations classify Lac Vieux Desert as a highly productive eutrophic lake. The pH of water in Lac Vieux Desert ranged from 6.5 to 9.5, and specific conductance ranged from 62 to 114 µs/cm. Chloride concentration was less than 1.5 mg/L, indicating little effect from septic-tank or road-salt input. Results indicate that the water can be classified as soft, with hardness concentrations reported as calcium carbonate ranging from 29 to 49 mg/L. Concentrations of calcium, magnesium, chloride, and other dissolved solids ranged from 47 to 77 mg/L. Alkalinity of Lac Vieux Desert ranged from 27 to 38 mg/L.Pervasive aquatic blooms, including a bloom noted during the September 2003 sampling, are apparently common in late summer. Biological productivity at Lac Vieux Desert does not appear to have changed appreciably between 1973 and 2004. In the current study, total phosphorus concentrations ranged from 0.01 to 0.064 mg/L and dissolved nitrite plus nitrate nitrogen concentrations ranged from at, or below detection limit to 0.052 mg/L. Overabundance of nutrients in Lac Vieux Desert, particularly nitrogen and phosphorus, could result in considerable degradation in lake-water quality.The estimated water balance includes the following inputs from the surrounding watershed: direct precipitation (35 percent); runoff, composed of streamflow and overland flow (50 percent); and ground-water flow (15 percent). Outputs from Lac Vieux Desert include streamflow into the Wisconsin River (68 percent) and evaporation from the lake surface (32 percent). Seasonal regulation of Lac Vieux Desert outflow results in an artificially high lake stage throughout the year, except from late winter to very early spring, prior to snowmelt and runoff. Regulation of Lac Vieux Desert outflow causes Wisconsin River streamflow to be artificially low during spring and summer and artificially high in fall and winter.Recent studies indicate that lake-level regulation over the past century may have affected wild rice growth and propagation in Lac Vieux Desert. As per licensing agreement between the Federal Energy Regulatory Commission and the Wisconsin Valley Improvement Company (operators of the dam at the outlet), the maximum lake level of Lac Vieux Desert was lowered about 0.8 feet to investigate the relation between lake-level regulation and propagation of wild rice from 2003 through 2012. Recent plantings of wild rice by the Lac Vieux Desert Band have been successful, indicating that suitable habitat and hydrologic regime were present in 2004-05.

The effects of Eph-ephrin mutations on pre-pulse inhibition in mice.

PubMed

Liuzzo, Andrea; Gray, Lincoln; Wallace, Matthew; Gabriele, Mark

2014-08-01

Eph-ephrin signaling is known to be important in directing topographic projections in the afferent auditory pathway, including connections to various subdivisions of the inferior colliculus (IC). The acoustic startle-response (ASR) is a reliable reflexive behavioral response in mammals elicited by an unexpected intense acoustic startle-eliciting stimulus (ES). It is mediated by a sub-cortical pathway that includes the IC. The ASR amplitude can be measured with an accelerometer under the subject and can be decreased in amplitude by presenting a less intense, non-startling stimulus 5-300ms before the ES. This reflexive decrement in ASR is called pre-pulse inhibition (PPI) and indicates that the relatively soft pre-pulse was heard. PPI is a general trait among mammals. Mice have been used recently to study this response and to reveal how genetic mutations affect neural circuits and hence the ASR and PPI. In this experiment, we measured the effect of Eph-ephrin mutations using control mice (C57BL/6J), mice with compromised EphA4 signaling (EphA4(lacZ/+), EphA4(lacZ/lacZ)), and knockout ephrin-B3 mice (ephrin-B3 (+/-, -/-)). Control and EphA4(lacZ/+s)trains showed robust PPI (up to 75% decrement in ASR) to an offset of a 70dB SPL background noise at 50ms before the ES. Ephrin-B3 knockout mice and EphA4 homozygous mutants were only marginally significant in PPI (<25% decrement and <33% decrement, respectively) to the same conditions. This decrement in PPI highlights the importance of ephrin-B3 and EphA4 interactions in ordering auditory behavioral circuits. Thus, different mutations in certain members of the signaling family produce a full range of changes in PPI, from minimal to nearly maximal. This technique can be easily adapted to study other aspects of hearing in a wider range of mutations. Along with ongoing neuroanatomical studies, this allows careful quantification of how the auditory anatomical, physiological and now behavioral phenotype is affected by changes in Eph-ephrin expression and functionality. Copyright © 2014 Elsevier Inc. All rights reserved.

A novel strategy of integrated microarray analysis identifies CENPA, CDK1 and CDC20 as a cluster of diagnostic biomarkers in lung adenocarcinoma.

PubMed

Liu, Wan-Ting; Wang, Yang; Zhang, Jing; Ye, Fei; Huang, Xiao-Hui; Li, Bin; He, Qing-Yu

2018-07-01

Lung adenocarcinoma (LAC) is the most lethal cancer and the leading cause of cancer-related death worldwide. The identification of meaningful clusters of co-expressed genes or representative biomarkers may help improve the accuracy of LAC diagnoses. Public databases, such as the Gene Expression Omnibus (GEO), provide rich resources of valuable information for clinics, however, the integration of multiple microarray datasets from various platforms and institutes remained a challenge. To determine potential indicators of LAC, we performed genome-wide relative significance (GWRS), genome-wide global significance (GWGS) and support vector machine (SVM) analyses progressively to identify robust gene biomarker signatures from 5 different microarray datasets that included 330 samples. The top 200 genes with robust signatures were selected for integrative analysis according to "guilt-by-association" methods, including protein-protein interaction (PPI) analysis and gene co-expression analysis. Of these 200 genes, only 10 genes showed both intensive PPI network and high gene co-expression correlation (r > 0.8). IPA analysis of this regulatory networks suggested that the cell cycle process is a crucial determinant of LAC. CENPA, as well as two linked hub genes CDK1 and CDC20, are determined to be potential indicators of LAC. Immunohistochemical staining showed that CENPA, CDK1 and CDC20 were highly expressed in LAC cancer tissue with co-expression patterns. A Cox regression model indicated that LAC patients with CENPA + /CDK1 + and CENPA + /CDC20 + were high-risk groups in terms of overall survival. In conclusion, our integrated microarray analysis demonstrated that CENPA, CDK1 and CDC20 might serve as novel cluster of prognostic biomarkers for LAC, and the cooperative unit of three genes provides a technically simple approach for identification of LAC patients. Copyright © 2018 Elsevier B.V. All rights reserved.

Blazars in Hard X-rays

NASA Astrophysics Data System (ADS)

Ghisellini, Gabriele

2009-05-01

Although blazars are thought to emit most of their luminosity in the γ-ray band, there are subclasses of them very prominent in hard X-rays. These are the best candidates to be studied by Simbol-X. They are at the extremes of the blazar sequence, having very small or very high jet powers. The former are the class of TeV emitting BL Lacs, whose synchrotron emission often peaks at tens of keV or more. The latter are the blazars with the most powerful jets, have high black hole masses accreting at high (i.e. close to Eddington) rates. These sources are predicted to have their high energy peak even below the MeV band, and therefore are very promising candidates to be studied with Simbol-X.

Evidence for a QPO structure in the TeV and X-ray light curve during the 1997 high state γ emission of Mkn 501

NASA Astrophysics Data System (ADS)

Kranich, D.

1999-08-01

The BL Lac Object Mkn 501 was in a state of high activity in the TeV range in 1997. During this time Mkn 501 was observed by all Cherenkov-Telescopes of the HEGRA-Collaboration. Part of the data were also taken during moonshine thus providing a nearly continuous coverage for this object in the TeV-range. We have carried out a QPO analysis and found evidence for a 23 day periodicity. We applied the same analysis on the 'data by dwell' x-ray lightcurve from the RXTE/ASM database and found also evidence for the 23 day periodicity. The combined probability was -.

The 105 month Swift-BAT data release

NASA Astrophysics Data System (ADS)

Oh, Kyuseok; Koss, Michael; Markwardt, Craig B.; Schawinski, Kevin; Baumgartner, Wayne H.; Barthelmy, Scott D.; Cenko, Bradley; Gehrels, Neil; Mushotzky, Richard; Petulante, Abigail; Ricci, Claudio; Lien, Amy; Trakhtenbrot, Benny; NASA GSFC Swift BAT team, BAT AGN Spectroscopic Survey (BASS)

2018-01-01

We present a new catalog of hard X-ray sources detected in the first 105 months of observations with the Burst Alert Telescope (BAT) on board the Swift observatory. The 105 month Swift-BAT survey is a uniform hard X-ray all-sky survey with a sensitivity of 8.40×10-12 erg s-1 cm-2 over 90% of the sky and 7.24×10-12 erg s-1 cm-2 over 50% of the sky in the 14‑195 keV band. The Swift-BAT 105 month catalog provides 1632 (422 new detections) hard X-ray sources in the 14 ‑ 195 keV band above the 4.8σ significance level. Adding to the previously known hard X-ray sources, 34% (144/422) of the new detections are identified as Seyfert AGN in nearby galaxies (z < 0.2). The majority of the remaining identified sources are X-ray binaries (7%, 31) and blazars/BL Lac objects (10%, 43). As part of this new edition of the Swift-BAT catalog, we release eight-channel spectra and monthly sampled light curves for each object in the online journal and at the Swift-BAT 105 month Web site.

PG 1553+113: Five Years Of Observations With Magic

DOE PAGES

J., Aleksić

2012-03-05

We present the results of five years (2005-2009) of MAGIC observations of the BL Lac object PG 1553+113 at very high energies (VHEs; E > 100 GeV). Power-law fits of the individual years are compatible with a steady mean photon index Γ = 4.27 ± 0.14. In the last three years of data, the flux level above 150 GeV shows a clear variability (probability of constant flux < 0.001%). The flux variations are modest, lying in the range from 4% to 11% of the Crab Nebula flux. Simultaneous optical data also show only modest variability that seems to be correlatedmore » with VHE gamma-ray variability. We also performed a temporal analysis of (all available) simultaneous Fermi/Large Area Telescope data of PG 1553+113 above 1 GeV, which reveals hints of variability in the 2008-2009 sample. Finally, we present a combination of the mean spectrum measured at VHEs with archival data available for other wavelengths. The mean spectral energy distribution can be modeled with a one-zone synchrotron self-Compton model, which gives the main physical parameters governing the VHE emission in the blazar jet.« less

On the Jet Properties of γ-Ray-loud Active Galactic Nuclei

NASA Astrophysics Data System (ADS)

Chen, Liang

2018-04-01

Based on broadband spectral energy distributions (SEDs), we estimate the jet physical parameters of 1392 γ-ray-loud active galactic nuclei (AGNs), the largest sample so far. The (SED) jet power and magnetization parameter are derived for these AGNs. Out of these sources, the accretion disk luminosity of 232 sources and (extended) kinetic jet powers of 159 sources are compiled from archived papers. We find the following. (1) Flat-spectrum radio quasars (FSRQs) and BL Lacs are well separated by {{Γ }}=-0.127{log}{L}γ +8.18 in the γ-ray luminosity versus photon index plane with a success rate of 88.6%. (2) Most FSRQs present a (SED) jet power larger than the accretion power, which suggests that the relativistic jet-launching mechanism is dominated by the Blandford–Znajek process. This result confirms previous findings. (3) There is a significant anticorrelation between jet magnetization and a ratio of the (SED) jet power to the (extended) kinetic jet power, which, for the first time, provides supporting evidence for the jet energy transportation theory: a high-magnetization jet may more easily transport energy to a large scale than a low-magnetization jet.

Volatile compounds of Celta dry-cured 'lacón' as affected by cross-breeding with Duroc and Landrace genotypes.

PubMed

Lorenzo, José M; Fonseca, Sonia

2014-11-01

Dry-cured 'lacón' is a traditional cured meat product made in the north-west of Spain from the pigs' foreleg, with similar manufacturing process to that used in dry-cured ham. The aim of this study was to assess the influence of cross-breeding of Celta pig with Landrace or Duroc breeds on the formation of volatile compounds through the manufacture of 'lacón'. 'Lacón' from the crosses with Duroc presented lower final moisture (534 g kg(-1) ) and higher intra-muscular fat content [144 g kg(-1) dry matter (DM)] than 'lacón' from Celta pure breed (587 g kg(-1) and 36 g kg(-1) DM, respectively). Volatile compounds were extracted by solid-phase microextraction and analysed by gas chromatography-mass spectrometry. Volatile compounds from 'lacón' were affected by cross-breeding. The total amount of volatile compounds significantly (P < 0.001) increased during the manufacturing process, this increase being more marked in samples from the Landrace cross-breed. The most abundant group of flavour compounds at the end of the manufacturing process was esters in the three batches, followed by aldehydes, hydrocarbons and alcohols. The most abundant ester at the end of the process was hexanoic acid methyl ester, while the aldehyde found in a higher amount was hexanal. The profile of volatile compounds was affected by cross-breed, especially at the end of the 'lacón' dry-curing process. © 2014 Society of Chemical Industry.

Studies of BL Lacertae objects with the Einstein Observatory - The soft X-ray spectra of OJ 287 and PKS 0735+178

NASA Technical Reports Server (NTRS)

Madejski, Greg M.; Schwartz, Daniel A.

1988-01-01

Accurate, soft X-ray spectra of two BL Lac objects, OJ 287 and PKS 0735+178, are presented. The X-ray spectra are well described by a power-law model with a low-energy cutoff consistent with photoelectric absorption within the Galaxy. The best-fit values of the energy spectral index in the 0.2-4.0 keV band are 0.91 and 0.76 respectively. The X-ray flux from OJ 287 is variable by a ratio of three from low to high state; PKS 0735+178 shows no indication of X-ray variability. The X-ray emission in OJ 287 is interpreted to be due to the synchrotron process from a volume common with either a beamed radio component or a stationary optical component. In PKS 0735+178, where the X-ray emission is most likely due to the Compton process operating in one of the VLBI radio components. The synchrotron self-Compton process with modest kinematic Doppler factors predicts the measured X-ray flux from PKS 0735+178 and lower than the measured flux in OJ 287.

PKS 2155-304 relativistically beamed synchrotron radiation from BL LAC object

NASA Technical Reports Server (NTRS)

Urry, C. M.; Mushotzky, R. F.

1981-01-01

The newly discovered BL Lacertae object, PKS 2155-304, was observed with the medium and high intensity energy detectors of the HEAO-1 A2 experiment. The variability by a factor of two in less than a day reported by Snyder, et al (1979) is confirmed. Two spectra, obtained a year apart, while the satellite was in scanning mode, are well fit by simple power laws with energy spectral index alpha sub 1 equals approximately 1.4. A third spectrum, of higher statistical quality, obtained while the satellite was pointed at its source, has has two components. An acceptable fit was obtained using a two power law model, with indices alpha sub 1 equals 2.0 (+1.2, -0.6) and alpha sub 2 equals -1.5 (+1.5, -2.3). An interpretation of the overall spectrum from radio through X-rays in terms of a synchrotron self-Compton model gives a good description of the data if allowance is made for relativistic beaming. Thus, from a consideration of the spectrum, combined with an estimate of the size of the source, the presence of jets is inferred without their observation.

Use of lipolanthionine peptide, a toll-like receptor 2 inhibitor, enhances transdermal delivery efficiency.

PubMed

Chen, Bin; Liu, Da-Lie; Pan, Wen-Yan; Yang, Xiao-Hui; Shou, Jia-Bao; Wu, Ju-Hua; Mao, Qing-Long; Wang, Jia

2014-08-01

The transdermal delivery system (TDS) is able to obtain a systemic therapeutic effect by administration through the skin, which has low side effects and is able to maintain a sustained blood concentration. However, due to the barrier presented by the stratum corneum, numerous drugs have poor percutaneous permeability. Therefore, the improvement of skin permeability is key to TDS. The main method of promoting transdermal absorption is through the usage of penetration enhancers. Dimethyl sulfoxide (DMSO) is a commonly used penetration enhancer, which has anti‑inflammatory analgesic effects and is able to penetrate the skin. Retinoic acid (RA) and lipolanthionine peptide (LP) may also benefit the permeation efficiency of TDS. Therefore, the present study examined the function of DMSO, RA and LP as penetration enhancers in TDS. Firstly, the optimum concentration of DMSO was confirmed by detecting the expression of the LacZ gene in vitro. Secondly, different combinations of LP, RA and DMSO were applied to mouse skin to analyze the penetration enhancer combination with the greatest efficacy. All the animals were divided into five groups: The RA + LP + DMSO + pORF‑LacZ group, the RA + DMSO + pORF‑LacZ group, the LP + DMSO + pORF‑LacZ group, the DMSO + pORF-LacZ group and the control group. Skin was soaked in combinations of LP, RA and DMSO for seven days and then the pORF‑LacZ plasmids were daubed onto the skin once daily three days. On the 11th day, all the animals were sacrificed by cervical dislocation and the skin and blood samples were collected. The blood samples were used to detect the expression of the LacZ gene by quantitative polymerase chain reaction and the skin samples were used to detect the expression of claudin‑4 and zonula occluden‑1 (ZO‑1) proteins by immunohistochemistry and western blot analysis. The results demonstrated that the combination of LP, RA and DMSO exhibited the greatest transdermal delivery efficiency, which verified that RA and LP were able to increase the penetration effects. Following treatment with LP, the symptoms of dermal edema were relieved and the capillaries contracted, which suggested that LP was a safe and effective penetration enhancer able to reduce the side‑effects caused by DMSO. The present study provides a guideline for the synthesis of novel penetration enhancers.

Plasma concentrations of glucagon-like peptide 1 and 2 in calves fed calf starters containing lactose.

PubMed

Inabu, Y; Saegusa, A; Inouchi, K; Koike, S; Oba, M; Sugino, T

2017-11-01

The objective of this study was to evaluate the effects of lactose inclusion in calf starters on plasma glucagon-like peptide (GLP)-1 and GLP-2 concentrations and gastrointestinal tract development in calves. Holstein bull calves (n = 45) were raised on an intensified nursing program using milk replacer containing 28.0% CP and 15.0% fat, and were fed a texturized calf starter containing 0 (control), 5.0 (LAC5), or 10.0% (LAC10; n = 15 for each treatment) lactose on a DM basis. Lactose was included in the starter by partially replacing dry ground corn in pelleted portion of the starter. All calf starters were formulated with 23.1% CP. The ethanol-soluble carbohydrate concentrations of the control, LAC5, and LAC10 starters were 7.3, 12.3, and 16.8% on a DM basis, respectively. Starch concentrations of the control, LAC5, and LAC10 starters were 29.7, 27.0, and 21.4% on a DM basis, respectively. All calves were fed treatment calf starters ad libitum. Blood samples were obtained weekly from 1 to 11 wk of age, and used to measure plasma GLP-1, GLP-2, and insulin concentrations, serum β-hydroxybutyrate (BHB) concentration, and blood glucose concentration. At 80 d of age, calves were euthanized, and weights of the reticulorumen, omasum, abomasum, small intestine, and large intestine tissue were measured. Serum BHB concentration was higher for calves fed the LAC10 (171 μmol/L) starter than for those fed the control (151 μmol/L) and LAC5 (145 μmol/L) starters. Plasma GLP-1 and GLP-2 concentrations did not differ between treatments. However, relative to the baseline (1 wk of age), the plasma GLP-1 concentration was higher for the LAC10 (125.9%) than for the LAC5 (68.2%) and control (36.8%), and for the LAC5 than for the control (36.8%). Moreover, similar differences between treatments were observed for GLP-2 concentration relative to the baseline (88.2, 76.9, and 74.9% for LAC10, LAC5, and control treatments, respectively). The serum BHB concentration was positively correlated with the plasma GLP-1 concentration (r = 0.428). Furthermore, the plasma GLP-1 concentration was positively correlated with the insulin concentration (r = 0.793). The weights of the reticulorumen, omasum, abomasum, small intestine, and large intestine were not affected by the treatments. In conclusion, inclusion of lactose in calf starters resulted in higher plasma GLP-1 and GLP-2 concentrations, and BHB might be associated with higher plasma GLP-1 concentration. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

LacR Is a Repressor of lacABCD and LacT Is an Activator of lacTFEG, Constituting the lac Gene Cluster in Streptococcus pneumoniae

PubMed Central

Afzal, Muhammad; Shafeeq, Sulman

2014-01-01

Comparison of the transcriptome of Streptococcus pneumoniae strain D39 grown in the presence of either lactose or galactose with that of the strain grown in the presence of glucose revealed the elevated expression of various genes and operons, including the lac gene cluster, which is organized into two operons, i.e., lac operon I (lacABCD) and lac operon II (lacTFEG). Deletion of the DeoR family transcriptional regulator lacR that is present downstream of the lac gene cluster revealed elevated expression of lac operon I even in the absence of lactose. This suggests a function of LacR as a transcriptional repressor of lac operon I, which encodes enzymes involved in the phosphorylated tagatose pathway in the absence of lactose or galactose. Deletion of lacR did not affect the expression of lac operon II, which encodes a lactose-specific phosphotransferase. This finding was further confirmed by β-galactosidase assays with PlacA-lacZ and PlacT-lacZ in the presence of either lactose or glucose as the sole carbon source in the medium. This suggests the involvement of another transcriptional regulator in the regulation of lac operon II, which is the BglG-family transcriptional antiterminator LacT. We demonstrate the role of LacT as a transcriptional activator of lac operon II in the presence of lactose and CcpA-independent regulation of the lac gene cluster in S. pneumoniae. PMID:24951784

Accretion processes of radio galaxies at high energies

NASA Astrophysics Data System (ADS)

de Jong, Sandra

2013-10-01

AGN are the luminous (L>10^42 erg/s) cores of active galaxies, powered by accretion onto the central super massive black hole, either via an accretion disk or via a radiatively inefficient accretion flow. There are still several open questions, for example on the launching of jets, which are present in about 10% of the AGN. Another question appeared with the Fermi/LAT gamma-ray survey, which detected many blazars but also a small group of radio galaxies. Radio galaxies are postulated to be blazars where the observer sees the jet at an angle θ>10 degrees allowing a view of both jet and core, rather than only the jet as is the case with blazars. Radio galaxies are divided into two classes, depending on the radio luminosity of the jets. The Fanaroff-Riley I (FR-I) sources have jets that are bright near the core, where the FR-IIs display extended edge-brightened jets. The FR-I sources are connected to the BL Lacs, which are low-luminosity blazars. FR-II types are thought to be the parent population of the luminous FSRQ, which are also blazars. This thesis presents a study of gamma-ray bright radio galaxies. By analysing X-ray and gamma-ray data in addition to creating broad-band spectral energy distributions (SEDs), we studied two examples of this new class of sources. For the FR-II source 3C 111 we analysed Suzaku/XIS and PIN and INTEGRAL IBIS/ISGRI observations to create a X-ray spectrum. We also used a Swift/BAT spectrum from the 58-month survey. The 0.4-200 keV spectrum of the source shows both thermal, Seyfert-like signatures such as an iron K-α line, and non-thermal jet features. We also analysed gamma-ray data from Fermi/LAT. The gamma-ray and X-ray data are combined with historical radio, infrared and optical observations to build the SED, which can be well represented with a non-thermal jet model. The bolometric luminosity of 3C111 is rather low, and the SED model shows rather a BL Lac type than the expected FSRQ. The next source we studied is the nearby FR-I M87. This source has been detected ! in gamma-rays and in the TeV band, but so far not in the hard X-rays (>10 keV). The first part of our analysis was focused on setting an upper limit to the hard X-ray emission of this source, using INTEGRAL IBIS/ISGRI observations. In addition to the standard method we applied several techniques in the analysis process, such as pointing selection and shadowgram treatment, in order to decrease the noise level of the result. Using 5.1 Ms of ISGRI data we determined a 3σ upper limit to the average 20-60 keV flux of f < 3x10^-12 erg/cm^2/s. We have also analysed Suzaku/PIN observations, where we detected M87 for the first time in the hard X-ray band, with a flux of f=1.3x 10^-11 erg/cm^2/s. between 20 and 60 keV. This detection indicates a flare, since the flux is significantly higher than the derived average upper limit. We also analysed Fermi/LAT data and combined this with the X-ray upper limits and historical radio, infrared and optical observations to build a SED. The SED can be modelled as a BL Lac source, which is expected since M87 is a FR-I type. We then also examined the general aspects of gamma-ray bright radio galaxies. Most of these objects are of the FR-I type, and the core of at least one FR-II, 3C 111, is rather BL Lac-like than the expected FSRQ. For the other FR-II sources this might also be the case. The gamma-ray emission originates from the jet, similar as in the case of blazars. Due to the large jet angle, the emission is not observed to be boosted. However, since the gamma-ray emission originates near the black hole, either reflection or a large opening angle can explain the observations. In addition, I contributed to the study of a possible dark matter halo observed with Fermi/LAT in the vicinity of the Virgo cluster. Our work has shown that a population of point sources contributes to this emission. In this thesis, the result of an extended emission analysis and the search for possible counter parts of new sources are presented. ! Finally, the detection of two new X-ray sources using Swift is reported here for the first time. These sources, the BL Lac object BZB J1552+0850 and the Seyfert galaxy LSBC F727-V01, are both located within the 95% error circle of the Fermi/LAT source 2FGL J1551.9+0855. We analysed the X-ray data from the XRT and UV data from the UVOT. The likely counterpart of the Fermi source is rather the blazer BZB J1552+0850, since Seyfert galaxies are rarely gamma-ray emitters. To understand the gamma-ray bright radio galaxies, X-ray observations can be used to can characterise these sources. Using for example the new generation of instruments, such as NuSTAR and ASTRO-H, will help with their superior resolution to distinguish between thermal and non-thermal emission in the X-ray spectrum. Additionally, building SEDs from simultaneous multi-wavelength observations will help constrain the broad-band emission. This will also help to pinpoint the counter part of Fermi/LAT detected sources, which is not trivial due to the large uncertainty in position

Microbial β-Galactosidase of Pediococcus pentosaceus ID-7: Isolation, Cloning, and Molecular Characterization.

PubMed

Lee, Ji-Yeong; Kwak, Mi-Sun; Roh, Jong-Bok; Kim, Kwang; Sung, Moon-Hee

2017-03-28

Pediococcus pentosaceus ID-7 was isolated from kimchi, a Korean fermented food, and it showed high activity for lactose hydrolysis. The β-galactosidase of P. pentosaceus ID-7 belongs to the GH2 group, which is composed of two distinct proteins. The heterodimeric LacLM type of β-galactosidase found in P. pentosaceus ID-7 consists of two genes partially overlapped, lacL and lacM encoding LacL (72.2 kDa) and LacM (35.4 kDa). In this study, Escherichia coli MM294 was used for the production of LacL, LacM, and LacLM. These three types of recombinant proteins were expressed, purified, and characterized. The specific activities of LacLM and LacL were 339 and 31 U/mg, respectively. However, activity was not detected with LacM alone. The optimal pH of LacLM and LacL was pH 7.5 and pH 7.0, and the optimal temperature of LacLM and LacL was 40°C and 50°C, respectively. The optimal temperature changes indicate that LacLM is able to achieve higher activity at a relatively lower temperature. LacLM was strongly activated by Mg 2+ , Mn 2+ , and Zn 2+ , which was not true for LacL. Consistent with this, EDTA strongly inactivated LacLM and LacL, but the presence of reducing agents did not dramatically alter the activity. Taken together, multiple alignment of amino acid sequences and phylogenetic analysis results of LacL and LacM of P. pentosaceus ID-7 suggest the evolution of LacL into LacLM and that the use of divalent metal ions results in higher activity.

Louisiana SIP: LAC 33:III Ch. 5 Section 509. Prevention of Significant Deterioration; SIP effective 1989-05-08 (LAc49) and 1989-08-14 (LAc50) and 1991-07-01 (LAc57) and 1996-12-16 (LAc69) to 2011-08-17 (LAd36 - Revised)

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch. 5 Section 509. Prevention of Significant Deterioration; SIP effective 1989-05-08 (LAc49) and 1989-08-14 (LAc50) and 1991-07-01 (LAc57) and 1996-12-16 (LAc69) to 2011-08-17 (LAd36 - Revised)

THE RADIO/GAMMA-RAY CONNECTION IN ACTIVE GALACTIC NUCLEI IN THE ERA OF THE FERMI LARGE AREA TELESCOPE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ackermann, M.; Ajello, M.; Allafort, A.

We present a detailed statistical analysis of the correlation between radio and gamma-ray emission of the active galactic nuclei (AGNs) detected by Fermi during its first year of operation, with the largest data sets ever used for this purpose. We use both archival interferometric 8.4 GHz data (from the Very Large Array and ATCA, for the full sample of 599 sources) and concurrent single-dish 15 GHz measurements from the Owens Valley Radio Observatory (OVRO, for a sub sample of 199 objects). Our unprecedentedly large sample permits us to assess with high accuracy the statistical significance of the correlation, using amore » surrogate data method designed to simultaneously account for common-distance bias and the effect of a limited dynamical range in the observed quantities. We find that the statistical significance of a positive correlation between the centimeter radio and the broadband (E > 100 MeV) gamma-ray energy flux is very high for the whole AGN sample, with a probability of <10{sup -7} for the correlation appearing by chance. Using the OVRO data, we find that concurrent data improve the significance of the correlation from 1.6 x 10{sup -6} to 9.0 x 10{sup -8}. Our large sample size allows us to study the dependence of correlation strength and significance on specific source types and gamma-ray energy band. We find that the correlation is very significant (chance probability < 10{sup -7}) for both flat spectrum radio quasars and BL Lac objects separately; a dependence of the correlation strength on the considered gamma-ray energy band is also present, but additional data will be necessary to constrain its significance.« less

The radio/gamma-ray connection in active galactic nuclei in the era of the Fermi Large Area Telescope

DOE PAGES

Ackermann, M.; Ajello, M.; Allafort, A.; ...

2011-10-12

We present a detailed statistical analysis of the correlation between radio and gamma-ray emission of the active galactic nuclei (AGNs) detected by Fermi during its first year of operation, with the largest data sets ever used for this purpose. We use both archival interferometric 8.4 GHz data (from the Very Large Array and ATCA, for the full sample of 599 sources) and concurrent single-dish 15 GHz measurements from the Owens Valley Radio Observatory (OVRO, for a sub sample of 199 objects). Our unprecedentedly large sample permits us to assess with high accuracy the statistical significance of the correlation, using amore » surrogate data method designed to simultaneously account for common-distance bias and the effect of a limited dynamical range in the observed quantities. We find that the statistical significance of a positive correlation between the centimeter radio and the broadband (E > 100 MeV) gamma-ray energy flux is very high for the whole AGN sample, with a probability of <10 –7 for the correlation appearing by chance. Using the OVRO data, we find that concurrent data improve the significance of the correlation from 1.6 × 10 –6 to 9.0 × 10 –8. Our large sample size allows us to study the dependence of correlation strength and significance on specific source types and gamma-ray energy band. As a result, we find that the correlation is very significant (chance probability < 10 –7) for both flat spectrum radio quasars and BL Lac objects separately; a dependence of the correlation strength on the considered gamma-ray energy band is also present, but additional data will be necessary to constrain its significance.« less

The Radio/Gamma-Ray Connection in Active Galactic Nuclei in the Era of the Fermi Large Area Telescope

NASA Technical Reports Server (NTRS)

Ackermann, M.; Ajello, M.; Allafort, A.; Angelakis, E.; Axelsson, M.; Baldini, L.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Bellazzini, R.;

Disruption of LACCASE4 and 17 Results in Tissue-Specific Alterations to Lignification of Arabidopsis thaliana Stems[W

PubMed Central

Berthet, Serge; Demont-Caulet, Nathalie; Pollet, Brigitte; Bidzinski, Przemyslaw; Cézard, Laurent; Le Bris, Phillipe; Borrega, Nero; Hervé, Jonathan; Blondet, Eddy; Balzergue, Sandrine; Lapierre, Catherine; Jouanin, Lise

2011-01-01

Peroxidases have been shown to be involved in the polymerization of lignin precursors, but it remains unclear whether laccases (EC 1.10.3.2) participate in constitutive lignification. We addressed this issue by studying laccase T-DNA insertion mutants in Arabidopsis thaliana. We identified two genes, LAC4 and LAC17, which are strongly expressed in stems. LAC17 was mainly expressed in the interfascicular fibers, whereas LAC4 was expressed in vascular bundles and interfascicular fibers. We produced two double mutants by crossing the LAC17 (lac17) mutant with two LAC4 mutants (lac4-1 and lac4-2). The single and double mutants grew normally in greenhouse conditions. The single mutants had moderately low lignin levels, whereas the stems of lac4-1 lac17 and lac4-2 lac17 mutants had lignin contents that were 20 and 40% lower than those of the control, respectively. These lower lignin levels resulted in higher saccharification yields. Thioacidolysis revealed that disrupting LAC17 principally affected the deposition of G lignin units in the interfascicular fibers and that complementation of lac17 with LAC17 restored a normal lignin profile. This study provides evidence that both LAC4 and LAC17 contribute to the constitutive lignification of Arabidopsis stems and that LAC17 is involved in the deposition of G lignin units in fibers. PMID:21447792

The statistics of gravitational lenses. III - Astrophysical consequences of quasar lensing

NASA Technical Reports Server (NTRS)

Ostriker, J. P.; Vietri, M.

1986-01-01

The method of Schmidt and Green (1983) for calculating the luminosity function of quasars is combined with gravitational-lensing theory to compute expected properties of lensed systems. Multiple quasar images produced by galaxies are of order 0.001 of the observed quasars, with the numbers over the whole sky calculated to be (0.86, 120, 1600) to limiting B magnitudes of (16, 19, 22). The amount of 'false evolution' is small except for an interesting subset of apparently bright, large-redshift objects for which minilensing by starlike objects may be important. Some of the BL Lac objects may be in this category, with the galaxy identified as the parent object really a foreground object within which stars have lensed a background optically violent variable quasar.

Water- and Bed-Sediment Quality of Seguchie Creek and Selected Wetlands Tributary to Mille Lacs Lake in Crow Wing County, Minnesota, October 2003 to October 2006

USGS Publications Warehouse

Fallon, James D.; Yaeger, Christine S.

2009-01-01

Mille Lacs Lake and its tributaries, located in east-central Minnesota, are important resources to the public. In addition, many wetlands and lakes that feed Mille Lacs Lake are of high resource quality and vulnerable to degradation. Construction of a new four-lane expansion of U.S. Highway 169 has been planned along the western part of the drainage area of Mille Lacs Lake in Crow Wing County. Concerns exist that the proposed highway could affect the resource quality of surface waters tributary to Mille Lacs Lake. Baseline water- and bed-sediment quality characteristics of surface waters tributary to Mille Lacs Lake were needed prior to the proposed highway construction. The U.S. Geological Survey, in cooperation with the Minnesota Department of Transportation, characterized the water- and bed-sediment quality at selected locations that the proposed route intersects from October 2003 to October 2006. Locations included Seguchie Creek upstream and downstream from the proposed route and three wetlands draining to Mille Lacs Lake. The mean streamflow of Seguchie Creek increased between the two sites: flow at the downstream streamflow-gaging station of 0.22 cubic meter per second was 5.6 percent greater than the mean streamflow at the upstream streamflow-gaging station of 0.21 cubic meter per second. Because of the large amount of storage immediately upstream from both gaging stations, increases in flow were gradual even during intense precipitation. The ranges of most constituent concentrations in water were nearly identical between the two sampling sites on Seguchie Creek. No concentrations exceeded applicable water-quality standards set by the State of Minnesota. Dissolved-oxygen concentrations at the downstream gaging station were less than the daily minimum standard of 4.0 milligrams per liter for 6 of 26 measurements. Constituent loads in Seguchie Creek were greater at the downstream site than the upstream site for all measured, including dissolved chloride (1.7 percent), ammonia plus organic nitrogen (13 percent), total phosphorus (62 percent), and suspended sediment (11 percent) during the study. All constituents had seasonal peaks in spring and fall. The large loads during the fall resulted from unusually large precipitation and streamflow patterns. This caused the two greatest streamflow peaks at both sites to occur during October (2004 and 2005). In Seguchie Creek, bed-sediment concentrations of five metals and trace elements (arsenic, cadmium, chromium, lead, and zinc) exceeded the Interim Sediment Quality Guidelines (ISQG) set by the Canadian Council of Ministers of the Environment. Bed-sediment samples from the upstream site had more exceedances of ISQGs for metals and trace elements than did samples from the downstream site (seven and two exceedances, respectively). Bed-sediment samples from the downstream site had more exceedances of ISQGs (20 exceedances) for semivolatile organic compounds than did samples from the upstream site (8 exceedances), indicating different sources for organic compounds than for metals and trace elements. Concentrations of 11 semivolatile organic compounds exceeded ISQGs: ancenaphthene, acenaphthylene, anthracene, benzo[a]anthracene, benzo[a]pyrene, chrysene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene. In bed-sediment samples collected from three wetlands, concentrations of all six metals exceeded ISQGs: arsenic, cadmium, chromium, copper, lead, and zinc. Concentrations of three semivolatile organic compounds exceeded ISQGs: flouranthene, phenanthrene, and pyrene. Results indicate that areas appearing relatively undisturbed and of high resource value can have degraded quality from previous unknown land use.

Multiwavelength Observations of the Blazar BL Lacertae: A New Fast TeV Gamma-Ray Flare

NASA Astrophysics Data System (ADS)

Abeysekara, A. U.; Benbow, W.; Bird, R.; Brantseg, T.; Brose, R.; Buchovecky, M.; Buckley, J. H.; Bugaev, V.; Connolly, M. P.; Cui, W.; Daniel, M. K.; Falcone, A.; Feng, Q.; Finley, J. P.; Fortson, L.; Furniss, A.; Gillanders, G. H.; Gunawardhana, I.; Hütten, M.; Hanna, D.; Hervet, O.; Holder, J.; Hughes, G.; Humensky, T. B.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kertzman, M.; Krennrich, F.; Lang, M. J.; Lin, T. T. Y.; McArthur, S.; Moriarty, P.; Mukherjee, R.; O’Brien, S.; Ong, R. A.; Otte, A. N.; Park, N.; Petrashyk, A.; Pohl, M.; Pueschel, E.; Quinn, J.; Ragan, K.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Rulten, C.; Sadeh, I.; Santander, M.; Sembroski, G. H.; Shahinyan, K.; Wakely, S. P.; Weinstein, A.; Wells, R. M.; Wilcox, P.; Williams, D. A.; Zitzer, B.; The VERITAS Collaboration; Jorstad, S. G.; Marscher, A. P.; Lister, M. L.; Kovalev, Y. Y.; Pushkarev, A. B.; Savolainen, T.; Agudo, I.; Molina, S. N.; Gómez, J. L.; Larionov, V. M.; Borman, G. A.; Mokrushina, A. A.; Tornikoski, M.; Lähteenmäki, A.; Chamani, W.; Enestam, S.; Kiehlmann, S.; Hovatta, T.; Smith, P. S.; Pontrelli, P.

2018-04-01

Combined with measurements made by very-long-baseline interferometry, the observations of fast TeV gamma-ray flares probe the structure and emission mechanism of blazar jets. However, only a handful of such flares have been detected to date, and only within the last few years have these flares been observed from lower-frequency-peaked BL Lac objects and flat-spectrum radio quasars. We report on a fast TeV gamma-ray flare from the blazar BL Lacertae observed by the Very Energetic Radiation Imaging Telescope Array System (VERITAS). with a rise time of ∼2.3 hr and a decay time of ∼36 min. The peak flux above 200 GeV is (4.2 ± 0.6) × 10‑6 photon m‑2 s‑1 measured with a 4-minute-binned light curve, corresponding to ∼180% of the flux that is observed from the Crab Nebula above the same energy threshold. Variability contemporaneous with the TeV gamma-ray flare was observed in GeV gamma-ray, X-ray, and optical flux, as well as in optical and radio polarization. Additionally, a possible moving emission feature with superluminal apparent velocity was identified in Very Long Baseline Array observations at 43 GHz, potentially passing the radio core of the jet around the time of the gamma-ray flare. We discuss the constraints on the size, Lorentz factor, and location of the emitting region of the flare, and the interpretations with several theoretical models that invoke relativistic plasma passing stationary shocks.

FERMI LARGE AREA TELESCOPE VIEW OF THE CORE OF THE RADIO GALAXY CENTAURUS A

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abdo, A. A.; Ackermann, M.; Ajello, M.

2010-08-20

We present {gamma}-ray observations with the Large Area Telescope (LAT) on board the Fermi Gamma-Ray Space Telescope of the nearby radio galaxy Centaurus A (Cen A). The previous EGRET detection is confirmed, and the localization is improved using data from the first 10 months of Fermi science operation. In previous work, we presented the detection of the lobes by the LAT; in this work, we concentrate on the {gamma}-ray core of Cen A. Flux levels as seen by the LAT are not significantly different from that found by EGRET, nor is the extremely soft LAT spectrum ({Gamma} = 2.67 {+-}more » 0.10{sub stat} {+-} 0.08{sub sys} where the photon flux is {Phi} {proportional_to} E {sup -{Gamma}}). The LAT core spectrum, extrapolated to higher energies, is marginally consistent with the non-simultaneous HESS spectrum of the source. The LAT observations are complemented by simultaneous observations from Suzaku, the Swift Burst Alert Telescope and X-ray Telescope, and radio observations with the Tracking Active Galactic Nuclei with Austral Milliarcsecond Interferometry program, along with a variety of non-simultaneous archival data from a variety of instruments and wavelengths to produce a spectral energy distribution (SED). We fit this broadband data set with a single-zone synchrotron/synchrotron self-Compton model, which describes the radio through GeV emission well, but fails to account for the non-simultaneous higher energy TeV emission observed by HESS from 2004 to 2008. The fit requires a low Doppler factor, in contrast to BL Lac objects which generally require larger values to fit their broadband SEDs. This indicates that the {gamma}-ray emission originates from a slower region than that from BL Lac objects, consistent with previous modeling results from Cen A. This slower region could be a slower moving layer around a fast spine, or a slower region farther out from the black hole in a decelerating flow. The fit parameters are also consistent with Cen A being able to accelerate ultra-high energy cosmic-rays, as hinted at by results from the Auger observatory.« less

Fermi Large Area Telescope View of the Core of the Radio Galaxy Centaurus A

NASA Astrophysics Data System (ADS)

Abdo, A. A.; Ackermann, M.; Ajello, M.; Atwood, W. B.; Baldini, L.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Baughman, B. M.; Bechtol, K.; Bellazzini, R.; Berenji, B.; Blandford, R. D.; Bloom, E. D.; Bonamente, E.; Borgland, A. W.; Bouvier, A.; Brandt, T. J.; Bregeon, J.; Brez, A.; Brigida, M.; Bruel, P.; Buehler, R.; Buson, S.; Caliandro, G. A.; Cameron, R. A.; Cannon, A.; Caraveo, P. A.; Carrigan, S.; Casandjian, J. M.; Cavazzuti, E.; Cecchi, C.; Çelik, Ö.; Charles, E.; Chekhtman, A.; Cheung, C. C.; Chiang, J.; Ciprini, S.; Claus, R.; Cohen-Tanugi, J.; Colafrancesco, S.; Cominsky, L. R.; Conrad, J.; Costamante, L.; Davis, D. S.; Dermer, C. D.; de Angelis, A.; de Palma, F.; Silva, E. do Couto e.; Drell, P. S.; Dubois, R.; Dumora, D.; Falcone, A.; Farnier, C.; Favuzzi, C.; Fegan, S. J.; Finke, J.; Focke, W. B.; Fortin, P.; Frailis, M.; Fukazawa, Y.; Funk, S.; Fusco, P.; Gargano, F.; Gasparrini, D.; Gehrels, N.; Georganopoulos, M.; Germani, S.; Giebels, B.; Giglietto, N.; Giommi, P.; Giordano, F.; Giroletti, M.; Glanzman, T.; Godfrey, G.; Grandi, P.; Grenier, I. A.; Grondin, M.-H.; Grove, J. E.; Guillemot, L.; Guiriec, S.; Hadasch, D.; Harding, A. K.; Hase, Hayo; Hayashida, M.; Hays, E.; Horan, D.; Hughes, R. E.; Itoh, R.; Jackson, M. S.; Jóhannesson, G.; Johnson, A. S.; Johnson, T. J.; Johnson, W. N.; Kadler, M.; Kamae, T.; Katagiri, H.; Kataoka, J.; Kawai, N.; Kishishita, T.; Knödlseder, J.; Kuss, M.; Lande, J.; Latronico, L.; Lee, S.-H.; Lemoine-Goumard, M.; Llena Garde, M.; Longo, F.; Loparco, F.; Lott, B.; Lovellette, M. N.; Lubrano, P.; Makeev, A.; Mazziotta, M. N.; McConville, W.; McEnery, J. E.; Michelson, P. F.; Mitthumsiri, W.; Mizuno, T.; Moiseev, A. A.; Monte, C.; Monzani, M. E.; Morselli, A.; Moskalenko, I. V.; Murgia, S.; Müller, C.; Nakamori, T.; Naumann-Godo, M.; Nolan, P. L.; Norris, J. P.; Nuss, E.; Ohno, M.; Ohsugi, T.; Ojha, R.; Okumura, A.; Omodei, N.; Orlando, E.; Ormes, J. F.; Ozaki, M.; Pagani, C.; Paneque, D.; Panetta, J. H.; Parent, D.; Pelassa, V.; Pepe, M.; Pesce-Rollins, M.; Piron, F.; Plötz, C.; Porter, T. A.; Rainò, S.; Rando, R.; Razzano, M.; Razzaque, S.; Reimer, A.; Reimer, O.; Reposeur, T.; Ripken, J.; Ritz, S.; Rodriguez, A. Y.; Roth, M.; Ryde, F.; Sadrozinski, H. F.-W.; Sanchez, D.; Sander, A.; Scargle, J. D.; Sgrò, C.; Siskind, E. J.; Smith, P. D.; Spandre, G.; Spinelli, P.; Starck, J.-L.; Stawarz, L.; Strickman, M. S.; Suson, D. J.; Tajima, H.; Takahashi, H.; Takahashi, T.; Tanaka, T.; Thayer, J. B.; Thayer, J. G.; Thompson, D. J.; Tibaldo, L.; Torres, D. F.; Tosti, G.; Tramacere, A.; Uchiyama, Y.; Usher, T. L.; Vandenbroucke, J.; Vasileiou, V.; Vilchez, N.; Vitale, V.; Waite, A. P.; Wang, P.; Winer, B. L.; Wood, K. S.; Yang, Z.; Ylinen, T.; Ziegler, M.

2010-08-01

We present γ-ray observations with the Large Area Telescope (LAT) on board the Fermi Gamma-Ray Space Telescope of the nearby radio galaxy Centaurus A (Cen A). The previous EGRET detection is confirmed, and the localization is improved using data from the first 10 months of Fermi science operation. In previous work, we presented the detection of the lobes by the LAT; in this work, we concentrate on the γ-ray core of Cen A. Flux levels as seen by the LAT are not significantly different from that found by EGRET, nor is the extremely soft LAT spectrum (Γ = 2.67 ± 0.10stat ± 0.08sys where the photon flux is Φ vprop E -Γ). The LAT core spectrum, extrapolated to higher energies, is marginally consistent with the non-simultaneous HESS spectrum of the source. The LAT observations are complemented by simultaneous observations from Suzaku, the Swift Burst Alert Telescope and X-ray Telescope, and radio observations with the Tracking Active Galactic Nuclei with Austral Milliarcsecond Interferometry program, along with a variety of non-simultaneous archival data from a variety of instruments and wavelengths to produce a spectral energy distribution (SED). We fit this broadband data set with a single-zone synchrotron/synchrotron self-Compton model, which describes the radio through GeV emission well, but fails to account for the non-simultaneous higher energy TeV emission observed by HESS from 2004 to 2008. The fit requires a low Doppler factor, in contrast to BL Lac objects which generally require larger values to fit their broadband SEDs. This indicates that the γ-ray emission originates from a slower region than that from BL Lac objects, consistent with previous modeling results from Cen A. This slower region could be a slower moving layer around a fast spine, or a slower region farther out from the black hole in a decelerating flow. The fit parameters are also consistent with Cen A being able to accelerate ultra-high energy cosmic-rays, as hinted at by results from the Auger observatory.

VERITAS OBSERVATIONS OF THE BL LAC OBJECT PG 1553+113

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aliu, E.; Archer, A.; Beilicke, M.

2015-01-20

We present results from VERITAS observations of the BL Lac object PG 1553+113 spanning the years 2010, 2011, and 2012. The time-averaged spectrum, measured between 160 and 560 GeV, is well described by a power law with a spectral index of 4.33 ± 0.09. The time-averaged integral flux above 200 GeV measured for this period was (1.69 ± 0.06) × 10{sup –11} photons cm{sup –2} s{sup –1}, corresponding to 6.9% of the Crab Nebula flux. We also present the combined γ-ray spectrum from the Fermi Large Area Telescope and VERITAS covering an energy range from 100 MeV to 560 GeV.more » The data are well fit by a power law with an exponential cutoff at 101.9 ± 3.2 GeV. The origin of the cutoff could be intrinsic to PG 1553+113 or be due to the γ-ray opacity of our universe through pair production off the extragalactic background light (EBL). Given lower limits to the redshift of z > 0.395 based on optical/UV observations of PG 1553+113, the cutoff would be dominated by EBL absorption. Conversely, the small statistical uncertainties of the VERITAS energy spectrum have allowed us to provide a robust upper limit on the redshift of PG 1553+113 of z ≤ 0.62. A strongly elevated mean flux of (2.50 ± 0.14) × 10{sup –11} photons cm{sup –2} s{sup –1} (10.3% of the Crab Nebula flux) was observed during 2012, with the daily flux reaching as high as (4.44±0.71)×10{sup −11} photons cm{sup −2} s{sup −1} (18.3% of the Crab Nebula flux) on MJD 56048. The light curve measured during the 2012 observing season is marginally inconsistent with a steady flux, giving a χ{sup 2} probability for a steady flux of 0.03%.« less

FERMI Large Area Telescope View of the 1 Core of the Radio Galaxy Centaurus A

DOE PAGES

Abdo, A. A.; Ackermann, M.; Ajello, M.; ...

2010-07-29

We present γ-ray observations with the Large Area Telescope (LAT) on board the Fermi Gamma-Ray Space Telescope of the nearby radio galaxy Centaurus A (Cen A). The previous EGRET detection is confirmed, and the localization is improved using data from the first 10 months of Fermi science operation. In previous work, we presented the detection of the lobes by the LAT; in this work, we concentrate on the γ-ray core of Cen A. Flux levels as seen by the LAT are not significantly different from that found by EGRET, nor is the extremely soft LAT spectrum (Γ = 2.67 ±more » 0.10 stat ± 0.08 sys where the photon flux is Φ ∝ E –Γ). The LAT core spectrum, extrapolated to higher energies, is marginally consistent with the non-simultaneous HESS spectrum of the source. The LAT observations are complemented by simultaneous observations from Suzaku, the Swift Burst Alert Telescope and X-ray Telescope, and radio observations with the Tracking Active Galactic Nuclei with Austral Milliarcsecond Interferometry program, along with a variety of non-simultaneous archival data from a variety of instruments and wavelengths to produce a spectral energy distribution (SED). Here, we fit this broadband data set with a single-zone synchrotron/synchrotron self-Compton model, which describes the radio through GeV emission well, but fails to account for the non-simultaneous higher energy TeV emission observed by HESS from 2004 to 2008. The fit requires a low Doppler factor, in contrast to BL Lac objects which generally require larger values to fit their broadband SEDs. This indicates that the γ-ray emission originates from a slower region than that from BL Lac objects, consistent with previous modeling results from Cen A. This slower region could be a slower moving layer around a fast spine, or a slower region farther out from the black hole in a decelerating flow. The fit parameters are also consistent with Cen A being able to accelerate ultra-high energy cosmic-rays, as hinted at by results from the Auger observatory.« less

THE CONTRIBUTION OF FERMI -2LAC BLAZARS TO DIFFUSE TEV–PEV NEUTRINO FLUX

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aartsen, M. G.; Abraham, K.; Ackermann, M.

2017-01-20

The recent discovery of a diffuse cosmic neutrino flux extending up to PeV energies raises the question of which astrophysical sources generate this signal. Blazars are one class of extragalactic sources which may produce such high-energy neutrinos. We present a likelihood analysis searching for cumulative neutrino emission from blazars in the 2nd Fermi -LAT AGN catalog (2LAC) using IceCube neutrino data set 2009-12, which was optimized for the detection of individual sources. In contrast to those in previous searches with IceCube, the populations investigated contain up to hundreds of sources, the largest one being the entire blazar sample in themore » 2LAC catalog. No significant excess is observed, and upper limits for the cumulative flux from these populations are obtained. These constrain the maximum contribution of 2LAC blazars to the observed astrophysical neutrino flux to 27% or less between around 10 TeV and 2 PeV, assuming the equipartition of flavors on Earth and a single power-law spectrum with a spectral index of −2.5. We can still exclude the fact that 2LAC blazars (and their subpopulations) emit more than 50% of the observed neutrinos up to a spectral index as hard as −2.2 in the same energy range. Our result takes into account the fact that the neutrino source count distribution is unknown, and it does not assume strict proportionality of the neutrino flux to the measured 2LAC γ -ray signal for each source. Additionally, we constrain recent models for neutrino emission by blazars.« less

Trends in Public and Global Health Education among Nationally Recognized Undergraduate Liberal Arts Colleges in the United States.

PubMed

Robinson, Patrick A; Orroth, Kate K; Stutts, Lauren A; Baron, Patrick A; Wessner, David R

2018-05-01

The prevalence of public health and global health (PH/GH) curricular offerings appear to be increasing in terms of undergraduate curricula and in the context of liberal arts education in the United States. Liberal arts colleges (LACs) represent stand-alone institutions, which exclusively focus on undergraduate education. The objective of this study was to assess the prevalence of PH/GH study pathways and PH/GH course offerings among LACs. All LACs identified through the US News and World Report (USNWR) college rankings were contacted with a survey about the following: formal majors, minors, or concentrations in PH/GH; independent study (IS) pathways for PH/GH; specific PH/GH courses offered; and the number of students graduating in 2016, 2017, and 2018 with formal and IS degrees in PH/GH. Demographic characteristics of the colleges came from the USNWR database. Almost half (43%) of all LACs in our sample offer a PH/GH major, minor, concentration, or IS pathway. Almost all (90%) colleges offer at least one course in PH/GH. Approximately 2,000 students attending these LACs pursued or are pursuing graduation with majors, minors, or concentrations in PH/GH for the years 2016-2018. The number of students pursuing formal PH/GH programs has increased by 25% from 2016 to 2018. Student interest in public health is rising in U.S. LACs, with more students seeking formal curricular or IS PH degree pathways. Public health messages are prevalent even among institutions without formal programs. Colleges without programs should consider integrating public health into their curriculum.

Lightning Arrestor Connectors Production Readiness

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marten, Steve; Linder, Kim; Emmons, Jim

2008-10-20

The Lightning Arrestor Connector (LAC), part “M”, presented opportunities to improve the processes used to fabricate LACs. The A## LACs were the first production LACs produced at the KCP, after the product was transferred from Pinnellas. The new LAC relied on the lessons learned from the A## LACs; however, additional improvements were needed to meet the required budget, yield, and schedule requirements. Improvement projects completed since 2001 include Hermetic Connector Sealing Improvement, Contact Assembly molding Improvement, development of a second vendor for LAC shells, general process improvement, tooling improvement, reduction of the LAC production cycle time, and documention of themore » LAC granule fabrication process. This report summarizes the accomplishments achieved in improving the LAC Production Readiness.« less

Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture (Most Probably Number)

PubMed Central

Fatemeh, Dehghan; Reza, Zolfaghari Mohammad; Mohammad, Arjomandzadegan; Salomeh, Kalantari; Reza, Ahmari Gholam; Hossein, Sarmadian; Maryam, Sadrnia; Azam, Ahmadi; Mana, Shojapoor; Negin, Najarian; Reza, Kasravi Alii; Saeed, Falahat

2014-01-01

Objective To analyse molecular detection of coliforms and shorten the time of PCR. Methods Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number (MPN) method for 16 artificial and 101 field samples. The molecular method was also conducted on isolated coliforms from positive MPN samples; standard sample for verification of microbial method certificated reference material; isolated strains from certificated reference material and standard bacteria. The PCR and electrophoresis parameters were changed for reducing the operation time. Results Results of PCR for lacZ and uidA genes were similar in all of standard, operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex PCR. PCR results were confirmed by MPN culture method by sensitivity 86% (95% CI: 0.71-0.93). Also the total execution time, with a successful change of factors, was reduced to less than two and a half hour. Conclusions Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak city. It's recommended to be used at least as an initial screening test, and then the positive samples could be randomly tested by MPN. PMID:25182727

Metabolic engineering of Escherichia coli to produce 2'-fucosyllactose via salvage pathway of guanosine 5'-diphosphate (GDP)-l-fucose.

PubMed

Chin, Young-Wook; Seo, Nari; Kim, Jae-Han; Seo, Jin-Ho

2016-11-01

2'-Fucosyllactose (2-FL) is one of the key oligosaccharides in human milk. In the present study, the salvage guanosine 5'-diphosphate (GDP)-l-fucose biosynthetic pathway from fucose was employed in engineered Escherichia coli BL21star(DE3) for efficient production of 2-FL. Introduction of the fkp gene coding for fucokinase/GDP-l-fucose pyrophosphorylase (Fkp) from Bacteroides fragilis and the fucT2 gene encoding α-1,2-fucosyltransferase from Helicobacter pylori allows the engineered E. coli to produce 2-FL from fucose, lactose and glycerol. To enhance the lactose flux to 2-FL production, the attenuated, and deleted mutants of β-galactosidase were employed. Moreover, the 2-FL yield and productivity were further improved by deletion of the fucI-fucK gene cluster coding for fucose isomerase (FucI) and fuculose kinase (FucK). Finally, fed-batch fermentation of engineered E. coli BL21star(DE3) deleting lacZ and fucI-fucK, and expressing fkp and fucT2 resulted in 23.1 g/L of extracellular concentration of 2-FL and 0.39 g/L/h productivity. Biotechnol. Bioeng. 2016;113: 2443-2452. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

The Discovery of a High-Redshift Quasar without Emission Lines from Sloan Digital Sky Survey Commissioning Data.

PubMed

Fan; Strauss; Gunn; Lupton; Carilli; Rupen; Schmidt; Moustakas; Davis; Annis; Bahcall; Brinkmann; Brunner; Csabai; Doi; Fukugita; Heckman; Hennessy; Hindsley; Ivezic; Knapp; Lamb; Munn; Pauls; Pier; Rockosi; Schneider; Szalay; Tucker; York

1999-12-01

We report observations of a luminous unresolved object at redshift z=4.62, with a featureless optical spectrum redward of the Lyalpha forest region, discovered from Sloan Digital Sky Survey commissioning data. The redshift is determined by the onset of the Lyalpha forest at lambda approximately 6800 Å and a Lyman limit system at lambda=5120 Å. A strong Lyalpha absorption system with weak metal absorption lines at z=4.58 is also identified in the spectrum. The object has a continuum absolute magnitude of -26.6 at 1450 Å in the rest frame (h0=0.5, q0=0.5) and therefore cannot be an ordinary galaxy. It shows no radio emission (the 3 sigma upper limit of its flux at 6 cm is 60 µJy), indicating a radio-to-optical flux ratio at least as small as that of the radio-weakest BL Lacertae objects known. It is also not linearly polarized to a 3 sigma upper limit of 4% in the observed I band. Therefore, it is either the most distant BL Lac object known to date, with very weak radio emission, or a new type of unbeamed quasar, whose broad emission line region is very weak or absent.

CONSTRAINTS ON THE INTERGALACTIC MAGNETIC FIELD WITH GAMMA-RAY OBSERVATIONS OF BLAZARS

DOE PAGES

Finke, Justin D.; Reyes, Luis C.; Georganopoulos, Markos; ...

2015-11-12

Distant BL Lacertae objects emit γ rays which interact with the extragalactic background light (EBL), creating electron-positron pairs, and reducing the flux measured by ground-based imaging atmospheric Cherenkov telescopes (IACTs) at very-high energies (VHE). These pairs can Comptonscatter the cosmic microwave background, creating a γ-ray signature at slightly lower energies observable by the Fermi Large Area Telescope (LAT). This signal is strongly dependent on the intergalactic magnetic field (IGMF) strength (B) and its coherence length (LB). We use IACT spectra taken from the literature for 5 VHE-detected BL Lac objects, and combine it with LAT spectra for these sources tomore » constrain these IGMF parameters. Low B values can be ruled out by the constraint that the cascade flux cannot exceed that observed by the LAT. High values of B can be ruled out from the constraint that the EBL-deabsorbed IACT spectrum cannot be greater than the LAT spectrum extrapolated into the VHE band, unless the cascade spectrum contributes a sizable fraction of the LAT flux. We rule out low B values (B . 10 -19 G for LB ≥ 1 Mpc) at > 5σ in all trials with different EBL models and data selection, except when« less

Evaluating the potential of immobilized bacterial consortium for black liquor biodegradation.

PubMed

Paliwal, Rashmi; Uniyal, Shivani; Rai, J P N

2015-05-01

Two indigenous bacterial strains, Bacillus megaterium ETLB-1 (accession no. KC767548) and Pseudomonas plecoglossicida ETLB-3 (accession no. KC767547), isolated from soil contaminated with paper mill effluent, were co-immobilized on corncob cubes to investigate their biodegradation potential against black liquor (BL). Results exhibit conspicuous reduction in color and lignin of BL upto 913.46 Co-Pt and 531.45 mg l(-1), respectively. Reduction in chlorophenols up to 12 mg l(-1) was recorded with highest release of chloride ions, i.e., 1290 mg l(-1). Maximum enzyme activity for lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (LAC) was recorded as 5.06, 8.13, and 8.23 U ml(-1), respectively, during the treatment. Scanning electron microscopy (SEM) revealed successful immobilization of bacterial strains in porous structures of biomaterial. Gas chromatography/mass spectroscopy (GC/MS) showed formation of certain low molecular weight metabolites such as 4-hydroxy-benzoic acid, 3-hydroxy-4-methoxybenzaldehyde, ferulic acid, and t-cinnamic acid and removal of majority of the compounds (such as teratogenic phthalate derivatives) during the period of treatment. Results demonstrated that the indigenous bacterial consortium possesses excellent decolorization and lignin degradation capability which enables its commercial utilization in effluents treatment system.

The 105-Month Swift-BAT All-Sky Hard X-Ray Survey

NASA Technical Reports Server (NTRS)

Oh, Kyuseok; Koss, Michael; Markwardt, Craig B.; Schawinski, Kevin; Baumgartner, Wayne H.; Barthelmy, Scott D.; Cenko, S. Bradley; Gehrels, Neil; Mushotzky, Richard; Petulante, Abigail;

The 105-Month Swift-BAT All-sky Hard X-Ray Survey

NASA Astrophysics Data System (ADS)

Oh, Kyuseok; Koss, Michael; Markwardt, Craig B.; Schawinski, Kevin; Baumgartner, Wayne H.; Barthelmy, Scott D.; Cenko, S. Bradley; Gehrels, Neil; Mushotzky, Richard; Petulante, Abigail; Ricci, Claudio; Lien, Amy; Trakhtenbrot, Benny

2018-03-01

We present a catalog of hard X-ray sources detected in the first 105 months of observations with the Burst Alert Telescope (BAT) coded-mask imager on board the Swift observatory. The 105-month Swift-BAT survey is a uniform hard X-ray all-sky survey with a sensitivity of 8.40× {10}-12 {erg} {{{s}}}-1 {cm}}-2 over 90% of the sky and 7.24× {10}-12 {erg} {{{s}}}-1 {cm}}-2 over 50% of the sky in the 14–195 keV band. The Swift-BAT 105-month catalog provides 1632 (422 new detections) hard X-ray sources in the 14–195 keV band above the 4.8σ significance level. Adding to the previously known hard X-ray sources, 34% (144/422) of the new detections are identified as Seyfert active galactic nuclei (AGNs) in nearby galaxies (z< 0.2). The majority of the remaining identified sources are X-ray binaries (7%, 31) and blazars/BL Lac objects (10%, 43). As part of this new edition of the Swift-BAT catalog, we release eight-channel spectra and monthly sampled light curves for each object in the online journal and at the Swift-BAT 105-month website.

An X-ray survey of variable radio bright quasars

NASA Technical Reports Server (NTRS)

Henriksen, M. J.; Marshall, F. E.; Mushotzky, R. F.

1984-01-01

A sample consisting primarily of radio bright quasars was observed in X-rays with the Einstein Observatory for times ranging from 1500 to 5000 seconds. Detected sources had luminosities ranging from 0.2 to 41.0 x 10 to the 45th power ergs/sec in the 0.5 to 4.5 keV band. Three of the fourteen objects which were reobserved showed flux increases greater than a factor of two on a time scale greater than six months. No variability was detected during the individual observations. The optical and X-ray luminosities are correlated, which suggests a common origin. However, the relationship (L sub x is approximately L sub op to the (.89 + or - .15)) found for historic radio variables may be significantly different than that reported for other radio bright sources. Some of the observed X-ray fluxes were substantially below the predicted self-Compton flux, assuming incoherent synchrotron emission and using VLBI results to constrain the size of the emission region, which suggests relativistic expansion in these sources. Normal CIV emission in two of the sources with an overpredicted Compton component suggests that although they, like BL Lac objects, have highly relativistic material apparently moving at small angle to the line of sight, they have a smaller fraction of the continuum component in the beam.

A CCD Study of the Environment of Seyfert Galaxies. II. Testing the Interaction Hypothesis

NASA Astrophysics Data System (ADS)

De Robertis, M. M.; Yee, H. K. C.; Hayhoe, K.

1998-03-01

An analysis of the environment of a sample of 33 CfA Seyfert galaxies and a control sample of 45 nonactive galaxies matched in luminosity, redshift, and morphology to the Seyfert galaxies as reported in Paper I is presented. The covariance function amplitudes of the Seyfert and control samples are not statistically significantly different from one another and from the general field. Moreover, the companion frequency of the Seyfert galaxies, the probability of finding a companion galaxy brighter than -17.5 in R within 50 kpc (0.30 +/- 0.11), is not statistically significantly different from that for the nonactive control sample (0.23 +/- 0.09). The mean environment of Seyfert 1 galaxies is found to be different from that of Seyfert 2 galaxies at greater than the 95% confidence level, in the sense that the latter have a larger covariance amplitude. Such evidence is problematic for the Unified Model, which attributes spectroscopic differences between the classes to purely geometric effects on the order of parsec scales. It cannot, however, account for differences on the order of 100 kpc scales. It is argued that triggering of activity in galactic nuclei may involve a variety of mechanisms and may depend on the luminosity of the class. That is, while there is excellent evidence that QSOs, radio galaxies, and BL Lac objects inhabit environments significantly richer than the field, the same does not seem to be true for Seyfert galaxies and perhaps for LINERs. Finally, because a significant fraction of Seyfert host galaxies show little or no evidence for a recent merger, it is suggested that ``minor mergers,'' mergers that involve a gas-rich disk galaxy and a bound companion or satellite galaxy, may play a significant role in triggering activity in Seyfert galaxies.

Statins: cost analysis in Indian scenario from eight major clinical trials.

PubMed

Sanmukhani, J; Shah, V

2010-01-01

Coronary heart disease (CHD) is the leading cause of death in India resulting in loss of young Indians. Statins have proved to reduce the CHD mortality in various clinical trials. The aim of the study is to find the cost-effectiveness ratio (CER) for each major coronary event averted and a coronary death avoided by use of statins in different clinical settings based on the data from the major clinical trials on statins. Using electronic database and as per our inclusion and exclusion criteria we selected the West of Scotland Coronary Prevention Study (WOSCOPS), the Air Force Coronary Atherosclerosis Prevention Study (AFCAPS) and the Anglo-Scandinavian Cardiac Outcomes Trial--Lipid Lowering Arm (ASCOT-LLA) study for primary prevention; the Cholesterol and Recurrent Events Trial (CARE), the Long-term Intervention with Pravastatin in Ischemic Disease (LIPID) Study and the Scandinavian Simvastatin Survival Study (4S) for secondary prevention and two studies, the Heart Protection Study (HPS) and the Pravastatin in elderly individuals at risk of vascular disease (PROSPER) study for high-risk patients. The results of these studies were used for cost-effectiveness analysis of statins in different patient groups. Absolute risk reduction, Number Needed to Benefit (NNTB), NNTB/year for total sample and in subgroups of males, females and age >65 was derived. CER for branded and generic versions was calculated by using the prices of statins listed in Indian Drug Review Triple i. Cost-effectiveness ratio (CER) in primary prevention studies i.e., the WOSCOPS, the AFCAPS and the ASCOT-LLA was Rs. 25.8 lacs, Rs. 23.8 lacs and Rs. 7.9 lacs per major coronary event averted respectively. CER in secondary prevention studies i.e., the CARE and the LIPID was approximately Rs. 20 lacs per major coronary event averted while it was Rs. 52.4 lacs and Rs. 37 lacs per coronary heart disease (CHD) death avoided. CER from the 4S was Rs. 6.9 lacs per major coronary event and Rs. 16.9 lacs per CHD death averted. CER in the HPS and the PROSPER study was Rs. 17.9 lacs and Rs. 27.1 lacs per major coronary event avoided in high-risk patients. Cost associated with the use of statins is higher in primary prevention as compared to secondary prevention. More studies are needed to confirm the cost-effectiveness of statins to make any decision for health policy.

Radio and gamma-ray properties of extragalactic jets from the TANAMI sample

DOE PAGES

Böck, M.; Kadler, M.; Müller, C.; ...

2016-05-04

The TANAMI program has been observing parsec-scale radio jets of southern (declination south of - 30°) γ-ray bright AGN, simultaneously with Fermi/LAT monitoring of their γ-ray emission, via high-resolution radio imaging with Very Long Baseline Interferometry techniques. In this paper, we present the radio and γ-rayproperties of the TANAMI sources based on one year of contemporaneous TANAMI and Fermi/LAT data. A large fraction (72%) of the TANAMI sample can be associated with bright γ-ray sources for this time range. Association rates differ for different optical classes with all BL Lacs, 76% of quasars, and just 17% of galaxies detected bymore » the LAT. Upper limits were established on the γ-ray flux from TANAMI sources not detected by LAT. This analysis led to the identification of three new Fermi sources whose detection was later confirmed. The γ-ray and radio luminosities are related by L γ ∝ L r 0.89±0.04. The brightness temperatures of the radio cores increase with the average γ-ray luminosity and the presence of brightness temperatures above the inverse Compton limit implies strong Doppler boosting in those sources. The undetected sources have lower γ/radio luminosity ratios and lower contemporaneous brightness temperatures. Finally, unless the Fermi/LAT-undetected blazars are much γ-ray-fainter than the Fermi/LAT-detected sources, their γ-ray luminosity should not be significantly lower than the upper limits calculated here.« less

Influence of salt content and processing time on sensory characteristics of cooked "lacón".

PubMed

Purriños, Laura; Bermúdez, Roberto; Temperán, Sara; Franco, Daniel; Carballo, Javier; Lorenzo, José M

2011-04-01

The influence of salt content and processing time on the sensory properties of cooked "lacón" were determined. "Lacón" is a traditional dry-cured and ripened meat product made in the north-west of Spain from the fore leg of the pig, following a similar process to that of dry-cured ham. Six batches of "lacón" were salted with different amounts of salt (LS (3 days of salting), MS (4 days of salting) and HS (5 days of salting)) and ripened during two times (56 and 84 days of dry-ripening). Cured odour in all batches studied, red colour and rancid odour in MS and HS batches, flavour intensity in MS batch and fat yellowness, rancid flavour and hardness in the HS batch were significantly different with respect to the time of processing. Appearance, odour, flavour and texture were not significantly affected by the salt content (P>0.05). However, the saltiness score showed significant differences with respect to the salt levels in all studied batches (56 and 84 days of process). The principal component analysis showed that physicochemical traits were the most important ones concerning the quality of dry-cured "lacón" and offered a good separation of the mean samples according to the dry ripening days and salt level. © 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.

Study of the lactic acid bacteria throughout the manufacture of dry-cured lacón (a Spanish traditional meat product). Effect of some additives.

PubMed

Lorenzo, José M; García Fontán, María C; Cachaldora, Aida; Franco, Inmaculada; Carballo, Javier

2010-04-01

Total aerobic mesophilic microflora (on SPC agar), lactic acid bacteria (on MRS agar) and lactobacilli (on Rogosa agar) were enumerated in samples from the surface and the interior of the pieces throughout the manufacture of six batches of lacón. Three of the batches were made without additives and three with additives (glucose (2 g/kg), sodium nitrite (E(250)) (125 mg/kg), sodium nitrate (E(251)) (175 mg/kg), sodium ascorbate (E(301)) (500 mg/kg), and sodium citrate (E(331)) (100 mg/kg)). The counts decreased throughout the manufacturing process, particularly after the salting stage. The use of additives did not affect the counts or the evolution of the microbial groups, except for the lactobacilli, which were present in higher numbers in the batches with additives. In four batches (two without and two with additives), from MRS agar and from Rogosa agar plates, 10 colonies were randomly taken from each sampling point of each batch (five from the surface sample and five from the interior sample) and from each culture medium; a total of 224 strains from MRS agar, and 176 strains from Rogosa agar that were identified by classical methods. The MRS agar displayed moderate selectivity for the isolation of lactic acid bacteria, and only 59% of the isolated strains belonged to this microbial group. Homofermentative and facultative heterofermentative lactobacilli (particularly Lactobacillus curvatus and Lactobacillus sakei) were the most abundant species isolated on this medium. The selectivity of the Rogosa agar for lactobacilli was extremely high. The species of lactobacilli isolated on this medium at different stages of manufacture of the four batches of lacón were consistent with those isolated from MRS agar. The use of additives in the lacón did not appreciably affect the kinds and proportions of species isolated on either MRS agar or Rogosa agar.

Intestinal Microbiota Ecological Response to Oral Administrations of Hydrogen-Rich Water and Lactulose in Female Piglets Fed a Fusarium Toxin-Contaminated Diet.

PubMed

Zheng, Weijiang; Ji, Xu; Zhang, Qing; Yao, Wen

2018-06-16

The objective of the current experiment was to explore the intestinal microbiota ecological response to oral administrations of hydrogen-rich water (HRW) and lactulose (LAC) in female piglets fed a Fusarium mycotoxin-contaminated diet. A total of 24 individually-housed female piglets (Landrace × large × white; initial average body weight, 7.25 ± 1.02 kg) were randomly assigned to receive four treatments (six pigs/treatment): uncontaminated basal diet (negative control, NC), mycotoxin-contaminated diet (MC), MC diet + HRW (MC + HRW), and MC diet + LAC (MC + LAC) for 25 days. Hydrogen levels in the mucosa of different intestine segments were measured at the end of the experiment. Fecal scoring and diarrhea rate were recorded every day during the whole period of the experiment. Short-chain fatty acids (SCFAs) profiles in the digesta of the foregut and hindgut samples were assayed. The populations of selected bacteria and denaturing gradient gel electrophoresis (DGGE) profiles of total bacteria and methanogenic Archaea were also evaluated. Results showed that Fusarium mycotoxins not only reduced the hydrogen levels in the caecum but also shifted the SCFAs production, and populations and communities of microbiota. HRW treatment increased the hydrogen levels of the stomach and duodenum. HRW and LAC groups also had higher colon and caecum hydrogen levels than the MC group. Both HRW and LAC protected against the mycotoxin-contaminated diet-induced higher diarrhea rate and lower SCFA production in the digesta of the colon and caecum. In addition, the DGGE profile results indicated that HRW and LAC might shift the pathways of hydrogen-utilization bacteria, and change the diversity of intestine microbiota. Moreover, HRW and LAC administrations reversed the mycotoxin-contaminated diet-induced changing of the populations of Escherichia coli (E. coli) and Bifidobacterium in ileum digesta and hydrogen-utilizing bacteria in colon digesta.

MAXI/GSC detection of a rapid X-ray brightening from Mrk 421

NASA Astrophysics Data System (ADS)

Tachibana, Y.; Ueda, Y.; Negoro, H.; Ueno, S.; Tomida, H.; Ishikawa, M.; Sugawara, Y.; Isobe, N.; Shimomukai, R.; Mihara, T.; Sugizaki, M.; Nakahira, S.; Iwakiri, W.; Shidatsu, M.; Yatabe, F.; Takao, Y.; Matsuoka, M.; Kawai, N.; Sugita, S.; Yoshii, T.; Harita, S.; Muraki, Y.; Morita, K.; Yoshida, A.; Sakamoto, T.; Serino, M.; Kawakubo, Y.; Kitaoka, Y.; Hashimoto, T.; Tsunemi, H.; Yoneyama, T.; Nakajima, M.; Kawase, T.; Sakamaki, A.; Hori, T.; Tanimoto, A.; Oda, S.; Morita, T.; Yamada, S.; Tsuboi, Y.; Nakamura, Y.; Sasaki, R.; Kawai, H.; Sato, T.; Yamauchi, M.; Hanyu, C.; Hidaka, K.; Kawamuro, T.; Yamaoka, K.

2018-01-01

MAXI/GSC is detecting a bright X-ray flare from the BL Lac object Mrk 421. The MAXI daily fluxes for the last 5 days are following: MJD & emsp; 2-4 keV (mCrab) & emsp; 4-10 keV (mCrab) 58131 & emsp; 53 +- 5 & emsp; 52 +- 6 58132 & emsp; 34 +- 5 & emsp; 29 +- 5 58133 & emsp; 56 +- 5 & emsp; 53 +- 6 58134 & emsp; 91 +- 7 & emsp; 98 +- 7 58135 & emsp; 106 +- 8 & emsp; 124 +- 9 The current flux is comparable with the peak daily flux in the brightest X-ray flare from this object ever since the beginning of the MAXI observation (156 +- 11 mCrab in 1.5-10 keV on 2010 February 16, ATEL #2444; Isobe et al. 2010 PASJ 52, L55), and the X-ray brightening is still ongoing.

Internal absorption of gamma-rays in relativistic blobs of active galactic nuclei

NASA Astrophysics Data System (ADS)

Sitarek, Julian; Bednarek, Wlodek

2007-06-01

We investigate the production of gamma-rays in the inverse Compton (IC) scattering process by leptons accelerated inside relativistic blobs in jets of active galactic nuclei. Leptons are injected homogeneously inside the spherical blob and initiate IC e ± pair cascade in the synchrotron radiation (produced by the same population of leptons, SSC model), provided that the optical depth for gamma-rays is larger than unity. It is shown that for likely parameters internal absorption of gamma-rays has to be important. We suggest that new type of blazars might be discovered by the future simultaneous X-ray and γ-ray observations, showing peak emissions in the hard X-rays, and in the GeV γ-rays. Moreover, the considered scenario might be also responsible for the orphan X-ray flares recently reported from BL Lac type active galaxies.

QUASI-PERIODIC OSCILLATIONS OF {approx}15 MINUTES IN THE OPTICAL LIGHT CURVE OF THE BL LAC S5 0716+714

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rani, Bindu; Gupta, Alok C.; Joshi, U. C.

Over the course of 3 hr on 2008 December 27, we obtained optical (R band) observations of the blazar S5 0716+714 at a very fast cadence of 10 s. Using several different techniques, we find fluctuations with an approximately 15 minute quasi-period to be present in the first portion of these data at a >3{sigma} confidence level. This is the fastest quasi-periodic oscillation that has been claimed to be observed in any blazar at any wavelength. While these data are insufficient to strongly constrain models for such fluctuations, the presence of such a short timescale when the source is notmore » in a very low state seems to favor the action of turbulence behind a shock in the blazar's relativistic jet.« less

Gains in efficiency and scientific potential of continental climate reconstruction provided by the LRC LacCore Facility, University of Minnesota

NASA Astrophysics Data System (ADS)

Noren, A.; Brady, K.; Myrbo, A.; Ito, E.

2007-12-01

Lacustrine sediment cores comprise an integral archive for the determination of continental paleoclimate, for their potentially high temporal resolution and for their ability to resolve spatial variability in climate across vast sections of the globe. Researchers studying these archives now have a large, nationally-funded, public facility dedicated to the support of their efforts. The LRC LacCore Facility, funded by NSF and the University of Minnesota, provides free or low-cost assistance to any portion of research projects, depending on the specific needs of the project. A large collection of field equipment (site survey equipment, coring devices, boats/platforms, water sampling devices) for nearly any lacustrine setting is available for rental, and Livingstone-type corers and drive rods may be purchased. LacCore staff can accompany field expeditions to operate these devices and curate samples, or provide training prior to device rental. The Facility maintains strong connections to experienced shipping agents and customs brokers, which vastly improves transport and importation of samples. In the lab, high-end instrumentation (e.g., multisensor loggers, high-resolution digital linescan cameras) provides a baseline of fundamental analyses before any sample material is consumed. LacCore staff provide support and training in lithological description, including smear-slide, XRD, and SEM analyses. The LRC botanical macrofossil reference collection is a valuable resource for both core description and detailed macrofossil analysis. Dedicated equipment and space for various subsample analyses streamlines these endeavors; subsamples for several analyses may be submitted for preparation or analysis by Facility technicians for a fee (e.g., carbon and sulfur coulometry, grain size, pollen sample preparation and analysis, charcoal, biogenic silica, LOI, freeze drying). The National Lacustrine Core Repository now curates ~9km of sediment cores from expeditions around the world, and stores metadata and analytical data for all cores processed at the facility. Any researcher may submit sample requests for material in archived cores. Supplies for field (e.g., polycarbonate pipe, endcaps), lab (e.g., sample containers, pollen sample spike), and curation (e.g., D-tubes) are sold at cost. In collaboration with facility users, staff continually develop new equipment, supplies, and procedures as needed in order to provide the best and most comprehensive set of services to the research community.

Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes

NASA Astrophysics Data System (ADS)

Hiraki, Masahiko; Watanabe, Shokei; Chavas, Leonard M. G.; Yamada, Yusuke; Matsugaki, Naohiro; Igarashi, Noriyuki; Wakatsuki, Soichi; Fujihashi, Masahiro; Miki, Kunio; Baba, Seiki; Ueno, Go; Yamamoto, Masaki; Suzuki, Mamoru; Nakagawa, Atsushi; Watanabe, Nobuhisa; Tanaka, Isao

2010-06-01

To remotely control and automatically collect data in high-throughput X-ray data collection experiments, the Structural Biology Research Center at the Photon Factory (PF) developed and installed sample exchange robots PAM (PF Automated Mounting system) at PF macromolecular crystallography beamlines; BL-5A, BL-17A, AR-NW12A and AR-NE3A. We developed and installed software that manages the flow of the automated X-ray experiments; sample exchanges, loop-centering and X-ray diffraction data collection. The fully automated data collection function has been available since February 2009. To identify sample cassettes, PAM employs a two-dimensional bar code reader. New beamlines, BL-1A at the Photon Factory and BL32XU at SPring-8, are currently under construction as part of Targeted Proteins Research Program (TPRP) by the Ministry of Education, Culture, Sports, Science and Technology of Japan. However, different robots, PAM and SPACE (SPring-8 Precise Automatic Cryo-sample Exchanger), will be installed at BL-1A and BL32XU, respectively. For the convenience of the users of both facilities, pins and cassettes for PAM and SPACE are developed as part of the TPRP.

Lactose metabolism by Staphylococcus aureus: characterization of lacABCD, the structural genes of the tagatose 6-phosphate pathway.

PubMed Central

Rosey, E L; Oskouian, B; Stewart, G C

1991-01-01

The nucleotide and deduced amino acid sequences of the lacA and lacB genes of the Staphylococcus aureus lactose operon (lacABCDFEG) are presented. The primary translation products are polypeptides of 142 (Mr = 15,425) and 171 (Mr = 18,953) amino acids, respectively. The lacABCD loci were shown to encode enzymes of the tagatose 6-phosphate pathway through both in vitro studies and complementation analysis in Escherichia coli. A serum aldolase assay, modified to allow detection of the tagatose 6-phosphate pathway enzymes utilizing galactose 6-phosphate or fructose phosphate analogs as substrate, is described. Expression of both lacA and lacB was required for galactose 6-phosphate isomerase activity. LacC (34 kDa) demonstrated tagatose 6-phosphate kinase activity and was found to share significant homology with LacC from Lactococcus lactis and with both the minor 6-phosphofructokinase (PfkB) and 1-phosphofructokinase (FruK) from E. coli. Detection of tagatose 1,6-bisphosphate aldolase activity was dependent on expression of the 36-kDa protein specified by lacD. The LacD protein is highly homologous with LacD of L. lactis. Thus, the lacABCD genes comprise the tagatose 6-phosphate pathway and are cotranscribed with genes lacFEG, which specify proteins for transport and cleavage of lactose in S. aureus. PMID:1655695

A 400-year phytolith-based reconstruction of wild rice (Zizania palustris) abundance from Mud Lake core sediments, Fond du Lac Band of Lake Superior Chippewa Reservation, Minnesota, USA.

NASA Astrophysics Data System (ADS)

Munoz, R.; Caylor, E.; Yost, C. L.; Drake, C.; Ladwig, J. L.; Myrbo, A.; Howes, T.

2014-12-01

Wild rice (Zizania palustris L.) is an aquatic grass with spiritual and subsistence significance to Native people of the Great Lakes region of North America. Mud Lake (Mashkiigwaagamaag), located on the Fond du Lac Band of Lake Superior Chippewa Reservation in Carlton County, Minnesota, USA, once supported an extensive population of wild rice (manoomin). However, early 20th century attempts to ditch and drain surrounding wetlands for landuse intensification severely altered the natural hydrological system that supports wild rice. Fond du Lac Resource Management (FDLRM) technicians are currently working to increase the wild rice population in Mud Lake. As part of these efforts, this phytolith study was undertaken to better understand how wild rice abundance has fluctuated over the past 400 years, with particular emphasis on the 19th and 20th centuries. Phytoliths are microscopic opal silica plant remains that are incorporated into soils and lake sediments after the plant-parts that contain them decay. Wild rice produces phytolith morphotypes that are unequivocally diagnostic. Mud Lake core MNMN-MUD11-1C-1P-1 (46°43'38.39"N, 92°42'2.45"W) was piston cored by LacCore (National Lacustrine Core Facility) and FDLRM technicians on 24 May 2011. Initial core descriptions, multi-sensor core logging, phytolith sampling and phytolith extractions were completed during the summer of 2014 at LacCore. Wild rice phytolith identification and quantification was conducted on twelve samples using brightfield microscopy at 400x magnification. Wild rice phytolith concentration values ranged from 68 to 2,300 phytoliths/cm3. Wild rice accumulation rates ranged from 9 to 383 phytoliths/ cm2/yr, peaking in 1952 AD. Wild rice abundance in Mud Lake appears to be influenced by a complex set of variables that include anthropogenic disturbance, climatic events and aquatic plant community succession.

Genome-Wide Identification and Characterization of Novel Laccase Genes in the White-Rot Fungus Flammulina velutipes

PubMed Central

Kim, Hong-Il; Kwon, O-Chul; Kong, Won-Sik; Lee, Chang-Soo

2014-01-01

The aim of this study was to identify and characterize new Flammulina velutipes laccases from its whole-genome sequence. Of the 15 putative laccase genes detected in the F. velutipes genome, four new laccase genes (fvLac-1, fvLac-2, fvLac3, and fvLac-4) were found to contain four complete copper-binding regions (ten histidine residues and one cysteine residue) and four cysteine residues involved in forming disulfide bridges, fvLac-1, fvLac-2, fvLac3, and fvLac-4, encoding proteins consisting of 516, 518, 515, and 533 amino acid residues, respectively. Potential N-glycosylation sites (Asn-Xaa-Ser/Thr) were identified in the cDNA sequence of fvLac-1 (Asn-454), fvLac-2 (Asn-437 and Asn-455), fvLac-3 (Asn-111 and Asn-237), and fvLac4 (Asn-402 and Asn-457). In addition, the first 19~20 amino acid residues of these proteins were predicted to comprise signal peptides. Laccase activity assays and reverse transcription polymerase chain reaction analyses clearly reveal that CuSO4 affects the induction and the transcription level of these laccase genes. PMID:25606003

Behavioral characterization of mice deficient in the phosphodiesterase-10A (PDE10A) enzyme on a C57/Bl6N congenic background.

PubMed

Siuciak, Judith A; McCarthy, Sheryl A; Chapin, Douglas S; Martin, Ashley N; Harms, John F; Schmidt, Christopher J

2008-02-01

The phenotype of genetically modified animals is strongly influenced by both the genetic background of the animal as well as environmental factors. We have previously reported the behavioral and neurochemical characterization of PDE10A knockout mice maintained on a DBA1LacJ (PDE10A(DBA)) genetic background. The aim of the present studies was to assess the behavioral and neurochemical phenotype of PDE10A knockout mice on an alternative congenic C57BL/6N (PDE10A(C57)) genetic background. Consistent with our previous results, PDE10A(C57) knockout mice showed a decrease in exploratory locomotor activity and a delay in the acquisition of conditioned avoidance responding. Also consistent with previous studies, the elimination of PDE10A did not alter basal levels of striatal cGMP or cAMP or affect behavior in several other well-characterized behavioral assays. PDE10A(C57) knockout mice showed a blunted response to MK-801, although to a lesser degree than previously observed in the PDE10A(DBA) knockout mice, and no differences were observed following a PCP challenge. PDE10A(C57) knockout mice showed a significant change in striatal dopamine turnover, which was accompanied by an enhanced locomotor response to AMPH, These studies demonstrate that while many of the behavioral effects of the PDE10A gene deletion appear to be independent of genetic background, the impact of the deletion on behavior can vary in magnitude. Furthermore, the effects on the dopaminergic system appear to be background-dependent, with significant effects observed only in knockout mice on the C57BL6N genetic background.

Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis.

PubMed

Lü, Shiyou; Song, Tao; Kosma, Dylan K; Parsons, Eugene P; Rowland, Owen; Jenks, Matthew A

2009-08-01

Plant cuticle is an extracellular lipid-based matrix of cutin and waxes, which covers aerial organs and protects them from many forms of environmental stress. We report here the characterization of CER8/LACS1, one of nine Arabidopsis long-chain acyl-CoA synthetases thought to activate acyl chains. Mutations in LACS1 reduced the amount of wax in all chemical classes on the stem and leaf, except in the very long-chain fatty acid (VLCFA) class wherein acids longer than 24 carbons (C(24)) were elevated more than 155%. The C(16) cutin monomers on lacs1 were reduced by 37% and 22%, whereas the C(18) monomers were increased by 28% and 20% on stem and leaf, respectively. Amounts of wax and cutin on a lacs1-1 lacs2-3 double mutant were much lower than on either parent, and lacs1-1 lacs2-3 had much higher cuticular permeability than either parent. These additive effects indicate that LACS1 and LACS2 have overlapping functions in both wax and cutin synthesis. We demonstrated that LACS1 has synthetase activity for VLCFAs C(20)-C(30), with highest activity for C(30) acids. LACS1 thus appears to function as a very long-chain acyl-CoA synthetase in wax metabolism. Since C(16) but not C(18) cutin monomers are reduced in lacs1, and C(16) acids are the next most preferred acid (behind C(30)) by LACS1 in our assays, LACS1 also appears to be important for the incorporation of C(16) monomers into cutin polyester. As such, LACS1 defines a functionally novel acyl-CoA synthetase that preferentially modifies both VLCFAs for wax synthesis and long-chain (C(16)) fatty acids for cutin synthesis.

Differences in the INR evaluation of two different thromboplastins in patients with positivity to lupus anticoagulant in ongoing oral anticoagulation.

PubMed

Ferrazzi, Paola; Colombo, Anna; Di Micco, Pierpaolo; Lodigiani, Corrado; Librè, Luca; Rota, Lidia Luciana; Montanelli, Alessandro; Quaglia, Ilaria

2010-01-01

A possible interference between lupus anticoagulant (LAC), a well characterized clotting inhibitor, in the International Normalized Ratio (INR) determination during oral anticoagulation (OA) has been reported in the literature. Few data are available about the relationship between this kind of interference and the daily clinical management of oral anticoagulation. The aim of the study is to evaluate the role of two different thromboplastins-RecombiPlasTin 2G and HepatoComplex-in the determination of INR values of several patients' ongoing OA for a previous thrombotic disorder with and without positivity to LAC, and to evaluate possible interferences in the daily therapeutic approach. We selected 16 patients (13 females and 3 males, mean age 59 ± 16 years) with LAC positivity ongoing OA and 11 control subjects (7 females and 4 males, mean age 58 ± 14.5 years) with similar characteristics (ie, ethnic background and weight) with LAC negativity ongoing OA. 165 assays for INR determination were analyzed from both groups. Statistical analysis was performed using STATA 10 software. P values were considered significant if <0.05. Mean values of INR for patients with LAC positivity were 3.79 ± 1.63 when tested with RecombiPlasTin 2G vs 3.18 ± 1.15 when tested with HepatoComplex (P < 0.001, s); while mean values of INR for patients with antiphospholipid syndrome (APS) with LAC negativity were 3.54 ± 1.39 when tested with RecombiPlasTin 2G vs 3.23 ± 1.14 when tested with HepatoComplex (P < 0.002, s). An INR value > than 4.5 was found in 31/165 samples in 9 subjects, 8 patients with LAC positivity, and 1 control group subject with LAC negativity. There was a great difference in INR values in these subjects if we use the common thromboplastin (ie, RecombiPlasTin 2G) with a INR range varying from 5.14 ± 0.35 vs 3.79 ± 0.38 if we use another thromboplastin (ie, HepatoComplex) (P < 0.001, s). A change in the therapeutic approach for OA is possible in these cases because different INR values were obtained using different thromboplastins. Our data confirm that INR evaluation does not reveal significant changes also if tested with two different thromboplastins, for patients ongoing OA with and without LAC positivity, when the INR value is < than 4. Over this INR value there is a significant difference in patients with LAC positivity if we use a different thromboplastin for the INR determination. For this reason values obtained by RecombiPlasTin 2G need to be confirmed and matched with another thromboplastin (ie, HepatoComplex). This approach may be useful in order to have a good INR testing for the chronic long-term treatment with OA in particular in patients with LAC positivity.

Louisiana SIP: LAC 33:III Ch. 14 Subchap A, 1401 to 1415--Determining Conformity of General Federal Actions to State or Federal Implementation Plans; SIP effective 1996-11-12 (LAc67) and 1998-05-08 (LAc75)

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch. 14 Subchap A, 1401 to 1415--Determining Conformity of General Federal Actions to State or Federal Implementation Plans; SIP effective 1996-11-12 (LAc67) and 1998-05-08 (LAc75)

lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA.

PubMed

Marbach, Anja; Bettenbrock, Katja

2012-01-01

Most commonly used expression systems in bacteria are based on the Escherichia coli lac promoter. Furthermore, lac operon elements are used today in systems and synthetic biology. In the majority of the cases the gratuitous inducers IPTG or TMG are used. Here we report a systematic comparison of lac promoter induction by TMG and IPTG which focuses on the aspects inducer uptake, population heterogeneity and a potential influence of the transacetylase, LacA. We provide induction curves in E. coli LJ110 and in isogenic lacY and lacA mutant strains and we show that both inducers are substrates of the lactose permease at low inducer concentrations but can also enter cells independently of lactose permease if present at higher concentrations. Using a gfp reporter strain we compared TMG and IPTG induction at single cell level and showed that bimodal induction with IPTG occurred at approximately ten-fold lower concentrations than with TMG. Furthermore, we observed that lac operon induction is influenced by the transacetylase, LacA. By comparing two Plac-gfp reporter strains with and without a lacA deletion we could show that in the lacA(+) strain the fluorescence level decreased after few hours while the fluorescence further increased in the lacA(-) strain. The results indicate that through the activity of LacA the IPTG concentration can be reduced below an inducing threshold concentration-an influence that should be considered if low inducer amounts are used. Copyright © 2011 Elsevier B.V. All rights reserved.

Crystal Structure and Substrate Specificity of D-Galactose-6-Phosphate Isomerase Complexed with Substrates

PubMed Central

Lee, Jung-Kul; Pan, Cheol-Ho

2013-01-01

D-Galactose-6-phosphate isomerase from Lactobacillus rhamnosus (LacAB; EC 5.3.1.26), which is encoded by the tagatose-6-phosphate pathway gene cluster (lacABCD), catalyzes the isomerization of D-galactose-6-phosphate to D-tagatose-6-phosphate during lactose catabolism and is used to produce rare sugars as low-calorie natural sweeteners. The crystal structures of LacAB and its complex with D-tagatose-6-phosphate revealed that LacAB is a homotetramer of LacA and LacB subunits, with a structure similar to that of ribose-5-phosphate isomerase (Rpi). Structurally, LacAB belongs to the RpiB/LacAB superfamily, having a Rossmann-like αβα sandwich fold as has been identified in pentose phosphate isomerase and hexose phosphate isomerase. In contrast to other family members, the LacB subunit also has a unique α7 helix in its C-terminus. One active site is distinctly located at the interface between LacA and LacB, whereas two active sites are present in RpiB. In the structure of the product complex, the phosphate group of D-tagatose-6-phosphate is bound to three arginine residues, including Arg-39, producing a different substrate orientation than that in RpiB, where the substrate binds at Asp-43. Due to the proximity of the Arg-134 residue and backbone Cα of the α6 helix in LacA to the last Asp-172 residue of LacB with a hydrogen bond, a six-carbon sugar-phosphate can bind in the larger pocket of LacAB, compared with RpiB. His-96 in the active site is important for ring opening and substrate orientation, and Cys-65 is essential for the isomerization activity of the enzyme. Two rare sugar substrates, D-psicose and D-ribulose, show optimal binding in the LacAB-substrate complex. These findings were supported by the results of LacA activity assays. PMID:24015281

Genome sequences of two closely related strains of Escherichia coli K-12 GM4792.

PubMed

Zhang, Yan-Cong; Zhang, Yan; Zhu, Bi-Ru; Zhang, Bo-Wen; Ni, Chuan; Zhang, Da-Yong; Huang, Ying; Pang, Erli; Lin, Kui

2015-01-01

Escherichia coli lab strains K-12 GM4792 Lac(+) and GM4792 Lac(-) carry opposite lactose markers, which are useful for distinguishing evolved lines as they produce different colored colonies. The two closely related strains are chosen as ancestors for our ongoing studies of experimental evolution. Here, we describe the genome sequences, annotation, and features of GM4792 Lac(+) and GM4792 Lac(-). GM4792 Lac(+) has a 4,622,342-bp long chromosome with 4,061 protein-coding genes and 83 RNA genes. Similarly, the genome of GM4792 Lac(-) consists of a 4,621,656-bp chromosome containing 4,043 protein-coding genes and 74 RNA genes. Genome comparison analysis reveals that the differences between GM4792 Lac(+) and GM4792 Lac(-) are minimal and limited to only the targeted lac region. Moreover, a previous study on competitive experimentation indicates the two strains are identical or nearly identical in survivability except for lactose utilization in a nitrogen-limited environment. Therefore, at both a genetic and a phenotypic level, GM4792 Lac(+) and GM4792 Lac(-), with opposite neutral markers, are ideal systems for future experimental evolution studies.

Extraction of organic materials from red water by metal-impregnated lignite activated carbon.

PubMed

Wei, Fangfang; Zhang, Yihe; Lv, Fengzhu; Chu, Paul K; Ye, Zhengfang

2011-12-15

Extraction of organic materials from 2,4,6-trinitrotoluene (TNT) red water by lignite activated carbon (LAC) impregnated with Cu(2+), Ba(2+), Sn(2+), Fe(3+), Ca(2+) and Ag(+) was investigated. The affinity to organic materials in red water was found to follow the order: Cu/LAC>Sn/LAC>Ag/LAC>Ba/LAC>Fe/LAC>Ca/LAC, which was explained by the hard and soft acid base (HSAB) theory. Cu(2+) showed the best performance and several parameters were further studied. X-ray photoelectron spectroscopy (XPS) verified effective loading of Cu(2+) on the LAC surface. The water quality before and after treated by Cu/LAC was evaluated using high performance liquid chromatograph, Gas Chromatography/Mass Spectroscopy (GC/MS), UV-vis spectroscopy and other analyses. The extraction performances and mechanism of organic materials on Cu/LAC were investigated through static methods. The experimental results showed that Cu/LAC possessed stronger extraction ability for the sulfonated nitrotoluenes than the non-sulfonated nitrotoluenes, the kinetic data fitted the pseudo-second-order kinetic model well. In addition, the leaching out of Cu(2+) from Cu/LAC was found much lower in the 100 times diluted red water (0.074%) than in the raw water (10.201%). Column adsorptions with more concentrated red water were also studied. Finally, Cu/LAC was observed to possess excellent reusability as well. Copyright © 2011 Elsevier B.V. All rights reserved.

Discovery of Very High Energy Gamma-Ray Emission from Rbs 0723 with the Magic Telescopes

NASA Astrophysics Data System (ADS)

Mirzoyan, Razmik

2014-01-01

The MAGIC collaboration reports the discovery of very high energy (VHE; E>100 GeV) gamma-ray emission from RBS 0723 (RA: 08:47:12.9 DEC: +11:33:50, J2000.0) RBS 0723 is a BL Lac object at redshift z=0.198. The source has been detected by Fermi-LAT, in the Second Fermi-LAT source Catalogue (2FGL; Nolan et al. 2012) with F(>1 GeV) = (5.3+-1.2)e-10 cm^-2 s^-1 and with photon index 1.48+-0.16. It also belongs to the first Fermi-LAT catalog of >10 GeV sources (1FHL; Ackermann et al, 2013), showing a hard (photon index = 1.4 +- 0.4) and bright (photon flux = 9.6e-11 ph cm^-2 s^-1) emission above 10 GeV, and identified as a good candidate for VHE detection.

DERIVATION OF A RELATION FOR THE STEEPENING OF TeV-SELECTED BLAZAR {gamma}-RAY SPECTRA WITH ENERGY AND REDSHIFT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stecker, Floyd William; Scully, Sean T.

2010-02-01

We derive a relation for the steepening of blazar {gamma}-ray spectra between the multi-GeV Fermi energy range and the TeV energy range observed by atmospheric Cerenkov telescopes. The change in spectral index is produced by two effects: (1) an intrinsic steepening, independent of redshift, owing to the properties of emission and absorption in the source and (2) a redshift-dependent steepening produced by intergalactic pair production interactions of blazar {gamma}-rays with low-energy photons of the 'intergalactic background light' (IBL). Given this relation, with good enough data on the mean {gamma}-ray spectral energy distribution of TeV-selected BL Lac objects, the redshift evolutionmore » of the IBL can, in principle, be determined independently of stellar evolution models. We apply our relation to the results of new Fermi observations of TeV-selected blazars.« less

A change in the X-ray spectrum of MK 421

NASA Technical Reports Server (NTRS)

Mushotzky, R. F.; Boldt, E. A.; Holt, S. S.; Serlemitsos, P. J.

1979-01-01

HEAO-1 experiment A-2 observations of the BL Lac object MK421 in May 1978 show a marked spectral change from the OSO-8 observations of May 1977. The source was not detected above 10 keV in May 1978. The 2-10 keV spectrum could be well fit by a power law of energy slope 2.2 is less than or minus 4.2; thermal bremsstrahlung models with T less than 2 X 10 to the 7th power deg K are also acceptable. There was no indication of any low energy turnover, so that the inferred column density N sub H is less than 7 X 10 to the 21st power at/sq cm. The total flux is consistent with an extrapolation of the UV data from IUE, but the slope is not consistent with the UV slope. Possible models for the origin of the spectral transition are discussed.

OPTICAL SPECTRA OF CANDIDATE SOUTHERN HEMISPHERE INTERNATIONAL CELESTIAL REFERENCE FRAME (ICRF) RADIO SOURCES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Titov, O.; Jauncey, D. L.; Johnston, H. M.

2011-11-15

We present the results of spectroscopic observations of the optical counterparts of 47 southern radio sources from the candidate International Celestial Reference Catalogue as part of a very long baseline interferometry (VLBI) program to strengthen the celestial reference frame, especially in the south. We made the observations with the 3.58 m European Southern Observatory New Technology Telescope. We obtained redshifts for 30 quasars and one radio galaxy, with a further seven objects being probable BL Lac objects with featureless spectra. Of the remainder, four were clear misidentifications with Galactic stars and five had low signal-to-noise spectra and could not bemore » classified. These results, in combination with new VLBI data of the radio sources with redshifts more than 2, add significantly to the existing data needed to refine the distribution of source proper motions over the celestial sphere.« less

Linear Polarization Properties of Parsec-Scale AGN Jets

NASA Astrophysics Data System (ADS)

Pushkarev, Alexander; Kovalev, Yuri; Lister, Matthew; Savolainen, Tuomas; Aller, Margo; Aller, Hugh; Hodge, Mary

2017-12-01

We used 15 GHz multi-epoch Very Long Baseline Array (VLBA) polarization sensitive observations of 484 sources within a time interval 1996--2016 from the MOJAVE program, and also from the NRAO data archive. We have analyzed the linear polarization characteristics of the compact core features and regions downstream, and their changes along and across the parsec-scale active galactic nuclei (AGN) jets. We detected a significant increase of fractional polarization with distance from the radio core along the jet as well as towards the jet edges. Compared to quasars, BL Lacs have a higher degree of polarization and exhibit more stable electric vector position angles (EVPAs) in their core features and a better alignment of the EVPAs with the local jet direction. The latter is accompanied by a higher degree of linear polarization, suggesting that compact bright jet features might be strong transverse shocks, which enhance magnetic field regularity by compression.

From radio to TeV: the surprising spectral energy distribution of AP Librae

DOE PAGES

Sanchez, D. A.; Giebels, B.; Fortin, P.; ...

2015-10-17

Following the discovery of high-energy (HE; E > 10 MeV) and very-high-energy (VHE; E > 100 GeV) γ-ray emission from the low-frequency-peaked BL Lac (LBL) object AP Librae, its electromagnetic spectrum is studied over 60 octaves in energy. Contemporaneous data in radio, optical and UV together with the (non-simultaneous) γ-ray data are used to construct the most precise spectral energy distribution of this source. We found that the data was modelled with difficulties with single-zone homogeneous leptonic synchrotron self-Compton (SSC) radiative scenarios due to the unprecedented width of the HE component when compared to the lower-energy component. Furthermore, the twomore » other LBL objects also detected at VHE appear to have similar modelling difficulties. Nevertheless, VHE γ-rays produced in the extended jet could account for the VHE flux observed by HESS.« less

MULTIWAVELENGTH EVIDENCE FOR QUASI-PERIODIC MODULATION IN THE GAMMA-RAY BLAZAR PG 1553+113

DOE PAGES

Ackerman, M.

2015-11-10

We report for the first time a γ-ray and multiwavelength nearly-periodic oscillation in an active galactic nucleus. Using the Fermi Large Area Telescope (LAT) we have discovered an apparent quasi-periodicity in the γ-ray flux (E > 100 MeV) from the GeV/TeV BL Lac object PG 1553+113. The marginal significance of the 2.18±0.08 year-period γ-ray cycle is strengthened by correlated oscillations observed in radio and optical fluxes, through data collected in the OVRO, Tuorla, KAIT, and CSS monitoring programs and Swift UVOT. The optical cycle appearing in ~ 10 years of data has a similar period, while the 15 GHz oscillationmore » is less regular than seen in the other bands. Further long-term multi-wavelength monitoring of this blazar may discriminate among the possible explanations for this quasi-periodicity.« less

The Properties of Extragalactic Radio Jets

NASA Astrophysics Data System (ADS)

Finke, Justin

2018-01-01

I show that by assuming a standard Blandford-Konigl jet, it is possible to determine the speed (bulk Lorentz factor) and orientation (angle to the line of sight) of self-similar parsec-scale blazar jets by using four measured quantities: the core radio flux, the extended radio flux, the magnitude of the core shift between two frequencies, and the apparent jet opening angle. Once the bulk Lorentz factor and angle to the line of sight of a jet are known, it is possible to compute their Doppler factor, magnetic field, and intrinsic jet opening angle. I use data taken from the literature and marginalize over nuisance parameters associated with the electron distribution and equipartition, to compute these quantities, albeit with large errors. The results have implications for the resolution of the TeV BL Lac Doppler factor crisis and the production of jets from magnetically arrested disks.

EMITTING ELECTRONS AND SOURCE ACTIVITY IN MARKARIAN 501

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mankuzhiyil, Nijil; Ansoldi, Stefano; Persic, Massimo

2012-07-10

We study the variation of the broadband spectral energy distribution (SED) of the BL Lac object Mrk 501 as a function of source activity, from quiescent to flaring. Through {chi}{sup 2}-minimization we model eight simultaneous SED data sets with a one-zone synchrotron self-Compton (SSC) model, and examine how model parameters vary with source activity. The emerging variability pattern of Mrk 501 is complex, with the Compton component arising from {gamma}-e scatterings that sometimes are (mostly) Thomson and sometimes (mostly) extreme Klein-Nishina. This can be seen from the variation of the Compton to synchrotron peak distance according to source state. Themore » underlying electron spectra are faint/soft in quiescent states and bright/hard in flaring states. A comparison with Mrk 421 suggests that the typical values of the SSC parameters are different in the two sources: however, in both jets the energy density is particle-dominated in all states.« less

MULTIWAVELENGTH EVIDENCE FOR QUASI-PERIODIC MODULATION IN THE GAMMA-RAY BLAZAR PG 1553+113

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ackermann, M.; Buehler, R.; Ajello, M.

2015-11-10

We report for the first time a γ-ray and multiwavelength nearly periodic oscillation in an active galactic nucleus. Using the Fermi Large Area Telescope we have discovered an apparent quasi-periodicity in the γ-ray flux (E > 100 MeV) from the GeV/TeV BL Lac object PG 1553+113. The marginal significance of the 2.18 ± 0.08 year period γ-ray cycle is strengthened by correlated oscillations observed in radio and optical fluxes, through data collected in the Owens Valley Radio Observatory, Tuorla, Katzman Automatic Imaging Telescope, and Catalina Sky Survey monitoring programs and Swift-UVOT. The optical cycle appearing in ∼10 years of datamore » has a similar period, while the 15 GHz oscillation is less regular than seen in the other bands. Further long-term multiwavelength monitoring of this blazar may discriminate among the possible explanations for this quasi-periodicity.« less

Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus

PubMed Central

Andersen, Joakim M.; Barrangou, Rodolphe; Abou Hachem, Maher; Lahtinen, Sampo; Goh, Yong Jun; Svensson, Birte; Klaenhammer, Todd R.

2011-01-01

Probiotic microbes rely on their ability to survive in the gastrointestinal tract, adhere to mucosal surfaces, and metabolize available energy sources from dietary compounds, including prebiotics. Genome sequencing projects have proposed models for understanding prebiotic catabolism, but mechanisms remain to be elucidated for many prebiotic substrates. Although β-galactooligosaccharides (GOS) are documented prebiotic compounds, little is known about their utilization by lactobacilli. This study aimed to identify genetic loci in Lactobacillus acidophilus NCFM responsible for the transport and catabolism of GOS. Whole-genome oligonucleotide microarrays were used to survey the differential global transcriptome during logarithmic growth of L. acidophilus NCFM using GOS or glucose as a sole source of carbohydrate. Within the 16.6-kbp gal-lac gene cluster, lacS, a galactoside-pentose-hexuronide permease-encoding gene, was up-regulated 5.1-fold in the presence of GOS. In addition, two β-galactosidases, LacA and LacLM, and enzymes in the Leloir pathway were also encoded by genes within this locus and up-regulated by GOS stimulation. Generation of a lacS-deficient mutant enabled phenotypic confirmation of the functional LacS permease not only for the utilization of lactose and GOS but also lactitol, suggesting a prominent role of LacS in the metabolism of a broad range of prebiotic β-galactosides, known to selectively modulate the beneficial gut microbiota. PMID:22006318

Distinct Viral and Mutational Spectrum of Endemic Burkitt Lymphoma.

PubMed

Abate, Francesco; Ambrosio, Maria Raffaella; Mundo, Lucia; Laginestra, Maria Antonella; Fuligni, Fabio; Rossi, Maura; Zairis, Sakellarios; Gazaneo, Sara; De Falco, Giulia; Lazzi, Stefano; Bellan, Cristiana; Rocca, Bruno Jim; Amato, Teresa; Marasco, Elena; Etebari, Maryam; Ogwang, Martin; Calbi, Valeria; Ndede, Isaac; Patel, Kirtika; Chumba, David; Piccaluga, Pier Paolo; Pileri, Stefano; Leoncini, Lorenzo; Rabadan, Raul

2015-10-01

Endemic Burkitt lymphoma (eBL) is primarily found in children in equatorial regions and represents the first historical example of a virus-associated human malignancy. Although Epstein-Barr virus (EBV) infection and MYC translocations are hallmarks of the disease, it is unclear whether other factors may contribute to its development. We performed RNA-Seq on 20 eBL cases from Uganda and showed that the mutational and viral landscape of eBL is more complex than previously reported. First, we found the presence of other herpesviridae family members in 8 cases (40%), in particular human herpesvirus 5 and human herpesvirus 8 and confirmed their presence by immunohistochemistry in the adjacent non-neoplastic tissue. Second, we identified a distinct latency program in EBV involving lytic genes in association with TCF3 activity. Third, by comparing the eBL mutational landscape with published data on sporadic Burkitt lymphoma (sBL), we detected lower frequencies of mutations in MYC, ID3, TCF3 and TP53, and a higher frequency of mutation in ARID1A in eBL samples. Recurrent mutations in two genes not previously associated with eBL were identified in 20% of tumors: RHOA and cyclin F (CCNF). We also observed that polyviral samples showed lower numbers of somatic mutations in common altered genes in comparison to sBL specimens, suggesting dual mechanisms of transformation, mutation versus virus driven in sBL and eBL respectively.

Frequency of EBV LMP-1 Promoter and Coding Variations in Burkitt Lymphoma Samples in Africa and South America and Peripheral Blood in Uganda.

PubMed

Liao, Hsiao-Mei; Liu, Hebing; Lei, Heiyan; Li, Bingjie; Chin, Pei-Ju; Tsai, Shien; Bhatia, Kishor; Gutierrez, Marina; Epelman, Sidnei; Biggar, Robert J; Nkrumah, Francis; Neequaye, Janet; Ogwang, Martin D; Reynolds, Steven J; Lo, Shyh-Ching; Mbulaiteye, Sam M

2018-06-02

Epstein-Barr virus (EBV) is linked to several cancers, including endemic Burkitt lymphoma (eBL), but causal variants are unknown. We recently reported novel sequence variants in the LMP-1 gene and promoter in EBV genomes sequenced from 13 of 14 BL biopsies. Alignments of the novel sequence variants for 114 published EBV genomes, including 27 from BL cases, revealed four LMP-1 variant patterns, designated A to D. Pattern A variant was found in 48% of BL EBV genomes. Here, we used PCR-Sanger sequencing to evaluate 50 additional BL biopsies from Ghana, Brazil, and Argentina, and peripheral blood samples from 113 eBL cases and 115 controls in Uganda. Pattern A was found in 60.9% of 64 BL biopsies evaluated. Compared to PCR-negative subjects in Uganda, detection of Pattern A in peripheral blood was associated with eBL case status (odds ratio [OR] 31.7, 95% confidence interval: 6.8⁻149), controlling for relevant confounders. Variant Pattern A and Pattern D were associated with eBL case status, but with lower ORs (9.7 and 13.6, respectively). Our results support the hypothesis that EBV LMP-1 Pattern A may be associated with eBL, but it is not the sole associated variant. Further research is needed to replicate and elucidate our findings.

Prevalence and comparison of Streptococcus infantarius subsp. infantarius and Streptococcus gallolyticus subsp. macedonicus in raw and fermented dairy products from East and West Africa.

PubMed

Jans, Christoph; Kaindi, Dasel Wambua Mulwa; Böck, Désirée; Njage, Patrick Murigu Kamau; Kouamé-Sina, Sylvie Mireille; Bonfoh, Bassirou; Lacroix, Christophe; Meile, Leo

2013-10-15

Streptococcus infantarius subsp. infantarius (Sii) and Streptococcus gallolyticus subsp. macedonicus are members of the Streptococcus bovis/Streptococcus equinus complex (SBSEC) associated with human infections. SBSEC-related endocarditis was furthermore associated with rural residency in Southern Europe. SBSEC members are increasingly isolated as predominant species from fermented dairy products in Europe, Asia and Africa. African variants of Sii displayed dairy adaptations to lactose metabolism paralleling those of Streptococcus thermophilus including genome decay. In this study, the aim was to assess the prevalence of Sii and possibly other SBSEC members in dairy products of East and West Africa in order to identify their habitat, estimate their importance in dairy fermentation processes and determine geographic areas affected by this potential health risk. Presumptive SBSEC members were isolated on semi-selective M17 and SM agar media. Subsequent genotypic identification of isolates was based on rep-PCR fingerprinting and SBSEC-specific16S rRNA gene PCR assay. Detailed identification was achieved through application of novel primers enhancing the binding stringency in partial groES/groEL gene amplification and subsequent DNA sequencing. The presence of S. thermophilus-like lacS and lacZ genes in the SBSEC isolates was determined to elucidate the prevalence of this dairy adaptation. Isolates (n = 754) were obtained from 72 raw and 95 fermented milk samples from Côte d'Ivoire and Kenya on semi-selective agar media. Colonies of Sii were not detected from raw milk despite high microbial titers of approximately 10(6)CFU/mL on M17 agar medium. However, after spontaneous milk fermentation Sii was genotypically identified in 94.1% of Kenyan samples and 60.8% of Kenyan isolates. Sii prevalence in Côte d'Ivoire displayed seasonal variations in samples from 32.3% (June) to 40.0% (Dec/Jan) and isolates from 20.5% (June) to 27.7% (Dec/Jan) present at titers of 10(6)-10(8)CFU/mL. lacS and lacZ genes were detected in all Kenyan and 25.8% (June) to 65.4% (Dec/Jan) of Ivorian Sii isolates. Regional differences in prevalence of Sii and dairy adaptations were observed, but no clear effect of dairy animal, fermentation procedure and climate was revealed. Conclusively, the high prevalence of Sii in Kenya, Côte d'Ivoire in addition to Somalia, Sudan and Mali strongly indicates a pivotal role of Sii in traditional African dairy fermentations potentially paralleling that of typical western dairy species S. thermophilus. Putative health risks associated with the consumption of high amounts of live Sii and potential different degrees of evolutionary adaptation or ecological colonization require further epidemiologic and genomic investigations, particularly in Africa. © 2013.

Prevalence and comparison of Streptococcus infantarius subsp. infantarius and Streptococcus gallolyticus subsp. macedonicus in raw and fermented dairy products from East and West Africa

PubMed Central

Jans, Christoph; Kaindi, Dasel Wambua Mulwa; Böck, Désirée; Njage, Patrick Murigu Kamau; Kouamé-Sina, Sylvie Mireille; Bonfoh, Bassirou; Lacroix, Christophe; Meile, Leo

2016-01-01

Streptococcus infantarius subsp. infantarius (Sii) and Streptococcus gallolyticus subsp. macedonicus are members of the Streptococcus bovis/Streptococcus equinus complex (SBSEC) associated with human infections. SBSEC-related endocarditis was furthermore associated with rural residency in Southern Europe. SBSEC members are increasingly isolated as predominant species from fermented dairy products in Europe, Asia and Africa. African variants of Sii displayed dairy adaptations to lactose metabolism paralleling those of Streptococcus thermophilus including genome decay. In this study, the aim was to assess the prevalence of Sii and possibly other SBSEC members in dairy products of East and West Africa in order to identify their habitat, estimate their importance in dairy fermentation processes and determine geographic areas affected by this potential health risk. Presumptive SBSEC members were isolated on semi-selective M17 and SM agar media. Subsequent genotypic identification of isolates was based on rep-PCR fingerprinting and SBSEC-specific16S rRNA gene PCR assay. Detailed identification was achieved through application of novel primers enhancing the binding stringency in partial groES/groEL gene amplification and subsequent DNA sequencing. The presence of S. thermophilus-like lacS and lacZ genes in the SBSEC isolates was determined to elucidate the prevalence of this dairy adaptation. Isolates (n = 754) were obtained from 72 raw and 95 fermented milk samples from Côte d'Ivoire and Kenya on semi-selective agar media. Colonies of Sii were not detected from raw milk despite high microbial titers of approximately 106 CFU/mL on M17 agar medium. However, after spontaneous milk fermentation Sii was genotypically identified in 94.1% of Kenyan samples and 60.8% of Kenyan isolates. Sii prevalence in Côte d'Ivoire displayed seasonal variations in samples from 32.3% (June) to 40.0% (Dec/Jan) and isolates from 20.5% (June) to 27.7% (Dec/Jan) present at titers of 106–108 CFU/mL. lacS and lacZ genes were detected in all Kenyan and 25.8% (June) to 65.4% (Dec/Jan) of Ivorian Sii isolates. Regional differences in prevalence of Sii and dairy adaptations were observed, but no clear effect of dairy animal, fermentation procedure and climate was revealed. Conclusively, the high prevalence of Sii in Kenya, Côte d'Ivoire in addition to Somalia, Sudan and Mali strongly indicates a pivotal role of Sii in traditional African dairy fermentations potentially paralleling that of typical western dairy species S. thermophilus. Putative health risks associated with the consumption of high amounts of live Sii and potential different degrees of evolutionary adaptation or ecological colonization require further epidemiologic and genomic investigations, particularly in Africa. PMID:24131584

Identification of hemolysin BL-producing Bacillus cereus isolates by a discontinuous hemolytic pattern in blood agar.

PubMed Central

Beecher, D J; Wong, A C

1994-01-01

Bacillus cereus causes distinct exotoxin-mediated diarrheal and emetic food poisoning syndromes and a variety of nongastrointestinal infections. Evidence is accumulating that hemolysin BL is a major B. cereus virulence factor. We describe two methods for detection of hemolysin BL in crude samples and on primary culture media. In the first method, the highly unusual discontinuous hemolysis pattern that is characteristic of pure hemolysin BL was produced in sheep and calf blood agar around wells filled with crude culture supernatant from hemolysin BL-producing strains. In the second method, the pattern was formed surrounding colonies of hemolysin BL-producing strains grown on media consisting of nutrient agar, 0.15 M NaCl, 2% calf serum, and sheep or calf blood. Hemolysin BL production was detected with these methods in 41 of 62 (66%) previously identified B. cereus isolates and in 46 of 136 (34%) presumptive B. cereus isolates from soil. All nine isolates tested that were associated with diarrhea or nongastrointestinal illness were positive for hemolysin BL. The methods presented here are specific, simple, inexpensive, and applicable to the screening of large numbers of samples or isolates. Images PMID:8017944

Constraints on the Intergalactic Magnetic Field with Gamma-Ray Observations of Blazars

NASA Astrophysics Data System (ADS)

Finke, Justin D.; Reyes, Luis C.; Georganopoulos, Markos; Reynolds, Kaeleigh; Ajello, Marco; Fegan, Stephen J.; McCann, Kevin

2015-11-01

Distant BL Lacertae objects emit γ-rays that interact with the extragalactic background light (EBL), creating electron-positron pairs, and reducing the flux measured by ground-based imaging atmospheric Cherenkov telescopes (IACTs) at very-high energies (VHE). These pairs can Compton-scatter the cosmic microwave background, creating a γ-ray signature at slightly lower energies that is observable by the Fermi Large Area Telescope (LAT). This signal is strongly dependent on the intergalactic magnetic field (IGMF) strength (B) and its coherence length (LB). We use IACT spectra taken from the literature for 5 VHE-detected BL Lac objects and combine them with LAT spectra for these sources to constrain these IGMF parameters. Low B values can be ruled out by the constraint that the cascade flux cannot exceed that observed by the LAT. High values of B can be ruled out from the constraint that the EBL-deabsorbed IACT spectrum cannot be greater than the LAT spectrum extrapolated into the VHE band, unless the cascade spectrum contributes a sizable fraction of the LAT flux. We rule out low B values (B ≲ 10-19 G for LB ≥ 1 Mpc) at >5σ in all trials with different EBL models and data selection, except when using >1 GeV spectra and the lowest EBL models. We were not able to constrain high values of B.

The Effect of Blazar Spectral Breaks on the Blazar Contribution to the Extragalactic Gamma-Ray Background

NASA Technical Reports Server (NTRS)

Venters, Tonia M.; Pavlidou, Vasiliki

2011-01-01

The spectral shapes of the contributions of different classes of unresolved gamma-ray emitters can provide insight into their relative contributions to the extragalactic gamma-ray background (EGB) and the natures of their spectra at GeV energies, We calculate the spectral shapes of the contributions to the EGB arising from BL Lacertae type objects (BL Lacs) and flat-spectrum radio quasars (FSRQs) assuming blazar spectra can be described as broken power laws, We fit the resulting total blazar spectral shape to the Fermi Large Area Telescope measurements of the EGB, finding that the best-fit shape reproduces well the shape of the Fermi EGB for various break scenarios. We conclude that a scenario in which the contribution of blazars is dominant cannot be excluded on spectral grounds alone, even if spectral breaks are shown to be common among Fermi blazars. We also find that while the observation of a featureless (within uncertainties) power-law EGB spectrum by Fermi does not necessarily imply a single class of contributing unresolved sources with featureless individual spectra, such an observation and the collective spectra of the separate contributing populations determine the ratios of their contributions. As such, a comparison with studies including blazar gamma-ray luminosity functions could have profound implications for the blazar contribution to the EGB, blazar evolution, and blazar gamma-ray spectra and emission.

Multiwavelength monitoring of the BL Lacertae object PKS 2155-304. 3: Ground-based observations in 1991 November

NASA Technical Reports Server (NTRS)

Courvoisier, T. J.-L.; Blecha, A.; Bouchet, P.; Bratschi, P.; Carini, M. T.; Donahue, M.; Edelson, R.; Feigelson, E. D.; Filippenko, A. V.; Glass, I. S.

1995-01-01

We present ground-based observations of the BL Lac object PKS 2155-304 during 1991 November. These data were obtained as part of a large international campaign of observations spanning the electro-magnetic spectrum from the radio waves to the X-rays. The data presented here include radio and UBVRI fluxes, as well as optical polarimetry. The U to I data show the same behavior in all bands and that only upper limits to any lag can be deduced from the cross-correlation of the light curves. The spectral slope in the U-I domain remained constant on all epochs but 2. There is no correlation between changes in the spectral slope and large variations in the total or polarized flux. The radio flux variations did not follow the same pattern of variability as the optical and infrared fluxes. The polarized flux varied by a larger factor than the total flux. The variations of the polarized flux are poorly correlated with those of the total flux in the optical (and hence UV domain; see the accompanying paper by Edelson et al.) nor with those of the soft X-rays. We conclude that the variability of PKS 2155-304 in the optical and near-infrared spectral domains are easier to understand in the context of variable geometry or bulk Lorentz factor than of variable electron acceleration and cooling rates.

Louisiana SIP: LAC 33:III Ch. 7 - Table 2 - Ambient Air--Methods of Contaminant Measurements; SIP effective 1989-05-08 (LAc49) and 1989-08-14 (LAc50) to 2011-08-03 (LAd34 - Moved to Section 711 and revised [adds PM-2.5])

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch. 7 - Table 2 - Ambient Air--Methods of Contaminant Measurements; SIP effective 1989-05-08 (LAc49) and 1989-08-14 (LAc50) to 2011-08-03 (LAd34 - Moved to Section 711 and revised [adds PM-2.5])

Louisiana SIP: LAC 33:III Ch. 7 Section 709. Measurement of Concentrations PM10, SO2, Carbon Monoxide, Atmospheric Oxidants, Nitrogen Oxides, and Lead; SIP effective 1989-05-08 (LAc49) and 1989-08-14 (LAc50) to 2011-08-03 (LAd34 - Revised)

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch. 7 Section 709. Measurement of Concentrations PM10, SO2, Carbon Monoxide, Atmospheric Oxidants, Nitrogen Oxides, and Lead; SIP effective 1989-05-08 (LAc49) and 1989-08-14 (LAc50) to 2011-08-03 (LAd34 - Revised)

Distinct signatures for mutator sensitivity of lacZ reversions and for the spectrum of lacI/lacO forward mutations on the chromosome of nondividing Escherichia coli.

PubMed Central

Bharatan, Shanti M; Reddy, Manjula; Gowrishankar, J

2004-01-01

A conditional lethal galE(Ts)-based strategy was employed in Escherichia coli, first to eliminate all growth-associated chromosomal reversions in lacZ or forward mutations in lacI/lacO by incubation at the restrictive temperature and subsequently to recover (as papillae) spontaneous mutations that had arisen in the population of nondividing cells after shift to the permissive temperature. Data from lacZ reversion studies in mutator strains indicated that the products of all genes for mismatch repair (mutHLS, dam, uvrD), of some for oxidative damage repair (mutMT), and of that for polymerase proofreading (dnaQ) are required in dividing cells; some others for oxidative damage repair (mutY, nth nei) are required in both dividing and nondividing cells; and those for alkylation damage repair (ada ogt) are required in nondividing cells. The spectrum of lacI/lacO mutations in nondividing cells was distinguished both by lower frequencies of deletions and IS1 insertions and by the unique occurrence of GC-to-AT transitions at lacO +5. In the second approach to study mutations that had occurred in nondividing cells, lacI/lacO mutants were selected as late-arising papillae from the lawn of a galE+ strain; once again, transitions at lacO +5 were detected among the mutants that had been obtained from populations initially grown on poor carbon sources such as acetate, palmitate, or succinate. Our results indicate that the lacO +5 site is mutable only in nondividing cells, one possible mechanism for which might be that random endogenous alkylation (or oxidative) damage to DNA in these cells is efficiently corrected by the Ada Ogt (or Nth Nei) repair enzymes at most sites but not at lacO +5. Furthermore, the late-arising papillae from the second approach were composed almost exclusively of dominant lacI/lacO mutants. This finding lends support to "instantaneous gratification" models in which a spontaneous lesion, occurring at a random site in DNA of a nondividing cell, is most likely to be fixed as a mutation if it allows the cell to immediately exit the nondividing state. PMID:15020459

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sobolewska, M. A.; Siemiginowska, A.; Kelly, B. C.

We study the γ-ray variability of 13 blazars observed with the Fermi/Large Area Telescope (LAT). These blazars have the most complete light curves collected during the first four years of the Fermi sky survey. We model them with the Ornstein-Uhlenbeck (OU) process or a mixture of the OU processes. The OU process has power spectral density (PSD) proportional to 1/f {sup α} with α changing at a characteristic timescale, τ{sub 0}, from 0 (τ >> τ{sub 0}) to 2 (τ << τ{sub 0}). The PSD of the mixed OU process has two characteristic timescales and an additional intermediate region withmore » 0 < α < 2. We show that the OU model provides a good description of the Fermi/LAT light curves of three blazars in our sample. For the first time, we constrain a characteristic γ-ray timescale of variability in two BL Lac sources, 3C 66A and PKS 2155-304 (τ{sub 0} ≅ 25 days and τ{sub 0} ≅ 43 days, respectively, in the observer's frame), which are longer than the soft X-ray timescales detected in blazars and Seyfert galaxies. We find that the mixed OU process approximates the light curves of the remaining 10 blazars better than the OU process. We derive limits on their long and short characteristic timescales, and infer that their Fermi/LAT PSD resemble power-law functions. We constrain the PSD slopes for all but one source in the sample. We find hints for sub-hour Fermi/LAT variability in four flat spectrum radio quasars. We discuss the implications of our results for theoretical models of blazar variability.« less

Strategies for Obtaining Probability Samples of Homeless Youth

ERIC Educational Resources Information Center

Golinelli, Daniela; Tucker, Joan S.; Ryan, Gery W.; Wenzel, Suzanne L.

2015-01-01

Studies of homeless individuals typically sample subjects from few types of sites or regions within a metropolitan area. This article focuses on the biases that can result from such a practice. We obtained a probability sample of 419 homeless youth from 41 sites (shelters, drop-in centers, and streets) in four regions of Los Angeles County (LAC).…

Disentangling Hadronic and Leptonic Cascade Scenarios from the Very-High-Energy Gamma-Ray Emission of Distant Hard-Spectrum Blazars

DOE PAGES

Takami, Hajime; Murase, Kohta; Dermer, Charles D.

2013-06-26

We show that recent data from the Fermi Large Area Telescope have revealed about a dozen distant hard-spectrum blazars that have very-high-energy (VHE; ≳ 100 eV) photons associated with them, but most of them have not yet been detected by imaging atmospheric Cherenkov Telescopes. Most of these high-energy gamma-ray spectra, like those of other extreme high-frequency peaked BL Lac objects, can be well explained either by gamma rays emitted at the source or by cascades induced by ultra-high-energy cosmic rays, as we show specifically for KUV 00311–1938. We consider the prospects for detection of the VHE sources by the plannedmore » Cherenkov Telescope Array (CTA) and show how it can distinguish the two scenarios by measuring the integrated flux above ~500 GeV (depending on source redshift) for several luminous sources with z ≲ 1 in the sample. Strong evidence for the origin of ultra-high-energy cosmic rays could be obtained from VHE observations with CTA. Depending on redshift, if the often quoted redshift of KUV 00311–1938 (z = 0.61) is believed, then preliminary H.E.S.S. data favor cascades induced by ultra-high-energy cosmic rays. Lastly, accurate redshift measurements of hard-spectrum blazars are essential for this study.« less

[Retrospective analysis of the value of arterial blood lactate level and its clearance rate on the prognosis of septic shock patients].

PubMed

Bao, Lei; Zhang, Min; Yan, Peixia; Wu, Xiaoyan; Shao, Jun; Zheng, Ruiqiang

2015-01-01

To explore the prognostic value of arterial blood lactate ( Lac ) levels and lactate clearance rate ( LCR ) in the patients with septic shock. A retrospective study was conducted. Clinical data of 94 septic patients admitted in the Department of Critical Care Medicine in Subei People's Hospital from January 2011 to June 2014 were analyzed. The arterial blood Lac levels at the moment of diagnosis of septic shock ( incipient value, 0 hour ) and early-stage after treatment ( 3, 6 and 24 hours ) were reviewed, and individual LCR was calculated at 3, 6, 24 hours for each patient. According to the outcome in intensive care unit ( ICU ), patients were divided into survival group ( n = 48 ) and death group ( n = 46 ). The Lac and LCR at different time points in two groups were analyzed, and the relationships between them and outcome were analyzed. The receiver-operating characteristic ( ROC ) curve was plotted to assess the value of Lac and LCR at different time points for predicting the outcome. Lac level after treatment in survival group was significantly lower than incipient value, but there was no obvious change in death group. Compared with death group, early Lac levels ( mmol/L ) in survival group were significantly reduced ( 0 hour: 3.80±2.14 vs. 5.75±3.21, 3 hours: 2.05±1.04 vs. 5.03±2.53, 6 hours: 1.80±0.77 vs. 4.40±2.02, 24 hours: 1.35±0.43 vs. 4.90±2.72, P<0.05 or P<0.01 ), the LCR was significantly increased [ 3 hours: 50.00 ( 72.35 )% vs. 13.51 ( 20.67 )%, 6 hours: 41.43 ( 58.42 )% vs. 22.00 ( 22.31 )%, 24 hours: 58.73 ( 29.94 )% vs. 18.92 ( 47.28 )%, P<0.05 or P<0.01 ]. The Lac levels at all time points were positively correlated with the outcome, and 6-hour and 24-hour LCR were negatively correlated with the outcome. According to the incipient Lac level, patients were divided into low Lac group ( Lac<2 mmol/L ), mild Lac group ( Lac 2-3 mmol/L ) and high Lac group ( Lac ≥ 4 mmol/L ). The mortality in low Lac group, mild Lac group, high Lac group was gradually increased [ 23.07% ( 6/26 ), 50.00% ( 8/16 ), 61.54% ( 32/52 ), χ(2) = 10.270, P = 0.006 ]. ROC curves demonstrated that the area under ROC curve ( AUC ) of 24-hour Lac was the largest, 0.944, and it was more sensitive and specific in the prognosis evaluation ( 100% and 78.3%, respectively ). According to the cut-off value of 24-hour Lac as 2.35 mmol/L, patients were divided into high Lac and low Lac groups, and mortality rate in high Lac group was significantly higher than that in low Lac group [ 100.0% ( 36/36 ) vs. 17.24% ( 10/58 ), χ(2) = 30.441,P = 0.000 ]. The AUC of 24-hour LCR was the largest, 0.865, and it was more sensitive and specific for the prognosis evaluation ( 83.3% and 91.3%, respectively ). According to the cut-off value of 24-hour LCR as 36.8%, patients were divided into high LCR group and low LCR group, and mortality rate in low LCR group was significantly higher than that in high LCR group [ 84.00% ( 42/50 ) vs. 9.09% ( 4/44 ), χ(2) = 26.278, P = 0.000 ]. Early high Lac in patients with septic shock prompts a poor prognosis, and 24-hour Lac levels and LCR are indicators of assessment of clinical therapeutic effect and prognosis of patients with septic shock.

Unprecedented study of the broadband emission of Mrk 421 during flaring activity in March 2010

DOE PAGES

Aleksić, J.; Ansoldi, S.; Antonelli, L. A.; ...

2015-05-27

Because of its proximity, Mrk 421 is one of the best sources on which to study the nature of BL Lac objects. Its proximity allows us to characterize its broadband spectral energy distribution (SED). Here, the goal is to better understand the mechanisms responsible for the broadband emission and the temporal evolution of Mrk 421. These mechanisms may also apply to more distant blazars that cannot be studied with the same level of detail. A flare occurring in March 2010 was observed for 13 consecutive days (from MJD 55 265 to MJD 55 277) with unprecedented wavelength coverage from radiomore » to very high energy (VHE; E> 100 GeV) γ-rays with MAGIC, VERITAS, Whipple, Fermi-LAT, MAXI, RXTE, Swift, GASP-WEBT, and several optical and radio telescopes. We modeled the day-scale SEDs with one-zone and two-zone synchrotron self-Compton (SSC) models, investigated the physical parameters, and evaluated whether the observed broadband SED variability can be associated with variations in the relativistic particle population. As a result, the activity of Mrk 421 initially was high and then slowly decreased during the 13-day period. The flux variability was remarkable at the X-ray and VHE bands, but it was minor or not significant at the other bands. The variability in optical polarization was also minor. These observations revealed an almost linear correlation between the X-ray flux at the 2–10 keV band and the VHE γ-ray flux above 200 GeV, consistent with the γ-rays being produced by inverse-Compton scattering in the Klein-Nishina regime in the framework of SSC models. The one-zone SSC model can describe the SED of each day for the 13 consecutive days reasonably well, which once more shows the success of this standard theoretical scenario to describe the SEDs of VHE BL Lacs such as Mrk 421. This flaring activity is also very well described by a two-zone SSC model, where one zone is responsible for the quiescent emission, while the other smaller zone, which is spatially separated from the first, contributes to the daily variable emission occurring at X-rays and VHE γ-rays. The second blob is assumed to have a smaller volume and a narrow electron energy distribution with 3 × 104« less

The Origin of Mutants Under Selection: How Natural Selection Mimics Mutagenesis (Adaptive Mutation)

PubMed Central

Maisnier-Patin, Sophie; Roth, John R.

2015-01-01

Selection detects mutants but does not cause mutations. Contrary to this dictum, Cairns and Foster plated a leaky lac mutant of Escherichia coli on lactose medium and saw revertant (Lac+) colonies accumulate with time above a nongrowing lawn. This result suggested that bacteria might mutagenize their own genome when growth is blocked. However, this conclusion is suspect in the light of recent evidence that revertant colonies are initiated by preexisting cells with multiple copies the conjugative F′lac plasmid, which carries the lac mutation. Some plated cells have multiple copies of the simple F′lac plasmid. This provides sufficient LacZ activity to support plasmid replication but not cell division. In nongrowing cells, repeated plasmid replication increases the likelihood of a reversion event. Reversion to lac+ triggers exponential cell growth leading to a stable Lac+ revertant colony. In 10% of these plated cells, the high-copy plasmid includes an internal tandem lac duplication, which provides even more LacZ activity—sufficient to support slow growth and formation of an unstable Lac+ colony. Cells with multiple copies of the F′lac plasmid have an increased mutation rate, because the plasmid encodes the error-prone (mutagenic) DNA polymerase, DinB. Without DinB, unstable and stable Lac+ revertant types form in equal numbers and both types arise with no mutagenesis. Amplification and selection are central to behavior of the Cairns–Foster system, whereas mutagenesis is a system-specific side effect or artifact caused by coamplification of dinB with lac. Study of this system has revealed several broadly applicable principles. In all populations, gene duplications are frequent stable genetic polymorphisms, common near-neutral mutant alleles can gain a positive phenotype when amplified under selection, and natural selection can operate without cell division when variability is generated by overreplication of local genome subregions. PMID:26134316

Beware of your Cre-Ation: lacZ expression impairs neuronal integrity and hippocampus-dependent memory.

PubMed

Reichel, J M; Bedenk, B T; Gassen, N C; Hafner, K; Bura, S A; Almeida-Correa, S; Genewsky, A; Dedic, N; Giesert, F; Agarwal, A; Nave, K-A; Rein, T; Czisch, M; Deussing, J M; Wotjak, C T

2016-10-01

Expression of the lacZ-sequence is a widely used reporter-tool to assess the transgenic and/or transfection efficacy of a target gene in mice. Once activated, lacZ is permanently expressed. However, protein accumulation is one of the hallmarks of neurodegenerative diseases. Furthermore, the protein product of the bacterial lacZ gene is ß-galactosidase, an analog to the mammalian senescence-associated ß-galactosidase, a molecular marker for aging. Therefore we studied the behavioral, structural and molecular consequences of lacZ expression in distinct neuronal sub-populations. lacZ expression in cortical glutamatergic neurons resulted in severe impairments in hippocampus-dependent memory accompanied by marked structural alterations throughout the CNS. In contrast, GFP expression or the expression of the ChR2/YFP fusion product in the same cell populations did not result in either cognitive or structural deficits. GABAergic lacZ expression caused significantly decreased hyper-arousal and mild cognitive deficits. Attenuated structural and behavioral consequences of lacZ expression could also be induced in adulthood, and lacZ transfection in neuronal cell cultures significantly decreased their viability. Our findings provide a strong caveat against the use of lacZ reporter mice for phenotyping studies and point to a particular sensitivity of the hippocampus formation to detrimental consequences of lacZ expression. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mueller, Uwe; Darowski, Nora; Fuchs, Martin R.

2012-03-20

Three macromolecular crystallography (MX) beamlines at the Helmholtz-Zentrum Berlin (HZB) are available for the regional, national and international structural biology user community. The state-of-the-art synchrotron beamlines for MX BL14.1, BL14.2 and BL14.3 are located within the low-[beta] section of the BESSY II electron storage ring. All beamlines are fed from a superconducting 7 T wavelength-shifter insertion device. BL14.1 and BL14.2 are energy tunable in the range 5-16 keV, while BL14.3 is a fixed-energy side station operated at 13.8 keV. All beamlines are equipped with CCD detectors. BL14.1 and BL14.2 are in regular user operation providing about 200 beam days permore » year and about 600 user shifts to approximately 50 research groups across Europe. BL14.3 has initially been used as a test facility and was brought into regular user mode operation during the year 2010. BL14.1 has recently been upgraded with a microdiffractometer including a mini-[kappa] goniometer and an automated sample changer. Other user facilities include office space adjacent to the beamlines, a sample preparation laboratory, a biology laboratory (safety level 1) and high-end computing resources. In this article the instrumentation of the beamlines is described, and a summary of the experimental possibilities of the beamlines and the provided ancillary equipment for the user community is given.« less

Distinct Viral and Mutational Spectrum of Endemic Burkitt Lymphoma

PubMed Central

Mundo, Lucia; Laginestra, Maria Antonella; Fuligni, Fabio; Rossi, Maura; Zairis, Sakellarios; Gazaneo, Sara; De Falco, Giulia; Lazzi, Stefano; Bellan, Cristiana; Rocca, Bruno Jim; Amato, Teresa; Marasco, Elena; Etebari, Maryam; Ogwang, Martin; Calbi, Valeria; Ndede, Isaac; Patel, Kirtika; Chumba, David; Piccaluga, Pier Paolo; Pileri, Stefano; Leoncini, Lorenzo; Rabadan, Raul

2015-01-01

Endemic Burkitt lymphoma (eBL) is primarily found in children in equatorial regions and represents the first historical example of a virus-associated human malignancy. Although Epstein-Barr virus (EBV) infection and MYC translocations are hallmarks of the disease, it is unclear whether other factors may contribute to its development. We performed RNA-Seq on 20 eBL cases from Uganda and showed that the mutational and viral landscape of eBL is more complex than previously reported. First, we found the presence of other herpesviridae family members in 8 cases (40%), in particular human herpesvirus 5 and human herpesvirus 8 and confirmed their presence by immunohistochemistry in the adjacent non-neoplastic tissue. Second, we identified a distinct latency program in EBV involving lytic genes in association with TCF3 activity. Third, by comparing the eBL mutational landscape with published data on sporadic Burkitt lymphoma (sBL), we detected lower frequencies of mutations in MYC, ID3, TCF3 and TP53, and a higher frequency of mutation in ARID1A in eBL samples. Recurrent mutations in two genes not previously associated with eBL were identified in 20% of tumors: RHOA and cyclin F (CCNF). We also observed that polyviral samples showed lower numbers of somatic mutations in common altered genes in comparison to sBL specimens, suggesting dual mechanisms of transformation, mutation versus virus driven in sBL and eBL respectively. PMID:26468873

Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin

PubMed Central

Mueller, Uwe; Darowski, Nora; Fuchs, Martin R.; Förster, Ronald; Hellmig, Michael; Paithankar, Karthik S.; Pühringer, Sandra; Steffien, Michael; Zocher, Georg; Weiss, Manfred S.

2012-01-01

Three macromolecular crystallography (MX) beamlines at the Helmholtz-Zentrum Berlin (HZB) are available for the regional, national and international structural biology user community. The state-of-the-art synchrotron beamlines for MX BL14.1, BL14.2 and BL14.3 are located within the low-β section of the BESSY II electron storage ring. All beamlines are fed from a superconducting 7 T wavelength-shifter insertion device. BL14.1 and BL14.2 are energy tunable in the range 5–16 keV, while BL14.3 is a fixed-energy side station operated at 13.8 keV. All three beamlines are equipped with CCD detectors. BL14.1 and BL14.2 are in regular user operation providing about 200 beam days per year and about 600 user shifts to approximately 50 research groups across Europe. BL14.3 has initially been used as a test facility and was brought into regular user mode operation during the year 2010. BL14.1 has recently been upgraded with a microdiffractometer including a mini-κ goniometer and an automated sample changer. Additional user facilities include office space adjacent to the beamlines, a sample preparation laboratory, a biology laboratory (safety level 1) and high-end computing resources. In this article the instrumentation of the beamlines is described, and a summary of the experimental possibilities of the beamlines and the provided ancillary equipment for the user community is given. PMID:22514183

Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin.

PubMed

Jiao, Xiaoyu; Li, Guoqing; Wang, Yan; Nie, Fan; Cheng, Xi; Abdullah, Muhammad; Lin, Yi; Cai, Yongping

2018-04-11

Fungal laccases play important roles in the degradation of lignocellulose. Although some PoLac s have been reported in several studies, still no comprehensive bioinformatics study of the LAC family in Pleurotus ostreatus has been reported. In this study, we identified 12 laccase genes in the whole genome sequence of P. ostreatus and their physical characteristics, gene distribution, phylogenic relationships, gene structure, conserved motifs, and cis-elements were also analyzed. The expression patterns of 12 PoLac genes at different developmental stages and under different culture substrates were also analyzed. The results revealed that PoLac2 and PoLac12 may be involved in the degradation of lignin and the formation of the fruiting body, respectively. Subsequently, we overexpressed PoLac2 in P. ostreatus by the Agrobacterium tumefaciens -mediated transformation (ATMT) method. The transformants' laccase activity increased in varying degrees, and the gene expression level of PoLac2 in transformants was 2-8 times higher than that of the wild-type strain. Furthermore, the lignin degradation rate by transgenic fungus over 30 days was 2.36-6.3% higher than that of wild-type. Our data show that overexpression of PoLac2 significantly enhanced the lignin degradation of cotton-straw. To our knowledge, this study is the first report to demonstrate the functions of PoLac2 in P. ostreatus .

Pesticides in surface water, bed sediment, and ground water adjacent to commercial cranberry bogs, Lac du Flambeau Reservation, Vilas County, Wisconsin

USGS Publications Warehouse

Saad, David A.

2005-01-01

In samples from the Trout River, which is used as a source of water to maintain lake levels in the Corn Lakes, the only pesticides detected were the non-targeted compounds atrazine and deethyl atrazine, indicating it was not a source of targeted compounds detected in the Corn Lakes. Only two pesticides (chlorpyrifos and metolachlor) were detected in bed-sediment samples collected from the lakes; chlorpyrifos from Little Trout Lake and metolachlor from the Corn Lakes. Four pesticides (the targeted compounds napropamide and norflurazon and the non-targeted compounds atrazine and deethyl atrazine) were detected in ground-water samples from two of four sampled monitor wells. The highest ground-water concentrations (up to 0.14 ?g/L napropamide and 0.56 ?g/L norflurazon) were measured in samples from the monitoring well located directly downgradient from the Corn Lakes and commercial cranberry operations. No pesticides were detected in samples from the reference well located upgradient from the Corn Lakes and cranberry operations. Further study is needed to identify additional pesticides as well as chronic effects on aquatic organisms to determine whether cranberry-related pesticides affect the lake ecosystems of the Lac du Flambeau Reservation.

The Impact of Developmental Education at Triton College.

ERIC Educational Resources Information Center

Chand, Sunil

1985-01-01

Describes the following aspects of the Developmental Education Program at Triton College: student placement, courses, faculty selection, reading and writing instruction, mathematics instruction, the Learning Assistance Center (LAC), LAC tutoring, LAC special projects, LAC management, special needs assistance program for disabled students, and…

Determining the rate of forest conversion in Mato Grosso, Brazil, using Landsat MSS and AVHRR data

NASA Technical Reports Server (NTRS)

Nelson, Ross; Horning, Ned; Stone, Thomas A.

1987-01-01

AVHRR-LAC thermal data and Landsat MSS and TM spectral data were used to estimate the rate of forest clearing in Mato Grosso, Brazil, between 1981 and 1984. The Brazilian state was stratified into forest and nonforest. A list sampling procedure was used in the forest stratum to select Landsat MSS scenes for processing based on estimates of fire activity in the scenes. Fire activity in 1984 was estimated using AVHRR-LAC thermal data. State-wide estimates of forest conversion indicate that between 1981 and 1984, 353,966 ha + or - 77,000 ha (0.4 percent of the state area) were converted per year. No evidence of reforestation was found in this digital sample. The relationship between forest clearing rate (based on MSS-TM analysis) and fire activity (estimated using AVHRR data) was noisy (R-squared = 0.41). The results suggest that AVHRR data may be put to better use as a stratification tool than as a subsidiary variable in list sampling.

Lysines 72, 80 and 213 and aspartic acid 210 of the Lactococcus lactis LacR repressor are involved in the response to the inducer tagatose-6-phosphate leading to induction of lac operon expression.

PubMed

van Rooijen, R J; Dechering, K J; Niek, C; Wilmink, J; de Vos, W M

1993-02-01

Site-directed mutagenesis of the Lactococcus lactis lacR gene was performed to identify residues in the LacR repressor that are involved in the induction of lacABCDFEGX operon expression by tagatose-6-phosphate. A putative inducer binding domain located near the C-terminus was previously postulated based on homology studies with the Escherichia coli DeoR family of repressors, which all have a phosphorylated sugar as inducer. Residues within this domain and lysine residues that are charge conserved in the DeoR family were changed into alanine or arginine. The production of the LacR mutants K72A, K80A, K80R, D210A, K213A and K213R in the LacR-deficient L.lactis strain NZ3015 resulted in repressed phospho-beta-galactosidase (LacG) activities and decreased growth rates on lactose. Gel mobility shift assays showed that the complex between a DNA fragment carrying the lac operators and LacR mutants K72A, K80A, K213A and D210A did not dissociate in the presence of tagatose-6-phosphate, in contrast to wild type LacR. Other mutations (K62A/K63A, K72R, K73A, K73R, T212A, F214R, R216R and R216K) exhibited no gross effects on inducer response. The results strongly suggest that the lysines at positions 72, 80 and 213 and aspartic acid at position 210 are involved in the induction of lac operon expression by tagatose-6-phosphate.

An acid-stable bacterial laccase identified from the endophyte Pantoea ananatis Sd-1 genome exhibiting lignin degradation and dye decolorization abilities.

PubMed

Shi, Xiaowei; Liu, Qian; Ma, Jiangshan; Liao, Hongdong; Xiong, Xianqiu; Zhang, Keke; Wang, Tengfei; Liu, Xuanmin; Xu, Ting; Yuan, Shanshan; Zhang, Xin; Zhu, Yonghua

2015-11-01

Isolation and identification of a novel laccase (namely Lac4) with various industrial applications potentials from an endophytical bacterium. Endophyte Sd-1 cultured in rice straw showed intra- and extra-cellular laccase activities. Genomic analysis of Sd-1 identified four putative laccases, Lac1 to Lac4. However, only Lac4 contains the complete signature sequence of laccase and shares at most 64 % sequence identity with other characterized bacterial multi-copper oxidases. Recombinant Lac4 can oxidize non-phenolic and phenolic compounds under acidic conditions and at 30-50 °C; Km values of Lac4 for ABTS at pH 2.5 and for guaiacol at pH 4.5 were 1 ± 0.15 and 6.1 ± 1.7 mM, respectively. The activity of Lac4 was stimulated by 0.8 mM Cu(2+) and 5 mM Fe(2+). In addition, Lac4 could decolorize various synthetic dyes and exhibit the degradation rate of 38 % for lignin. The data suggest that Lac4 possesses promising biotechnological potentials.

Modeling the Lac repressor-operator assembly: The influence of DNA looping on Lac repressor conformation

PubMed Central

Swigon, David; Coleman, Bernard D.; Olson, Wilma K.

2006-01-01

Repression of transcription of the Escherichia coli Lac operon by the Lac repressor (LacR) is accompanied by the simultaneous binding of LacR to two operators and the formation of a DNA loop. A recently developed theory of sequence-dependent DNA elasticity enables one to relate the fine structure of the LacR–DNA complex to a wide range of heretofore-unconnected experimental observations. Here, that theory is used to calculate the configuration and free energy of the DNA loop as a function of its length and base-pair sequence, its linking number, and the end conditions imposed by the LacR tetramer. The tetramer can assume two types of conformations. Whereas a rigid V-shaped structure is observed in the crystal, EM images show extended forms in which two dimer subunits are flexibly joined. Upon comparing our computed loop configurations with published experimental observations of permanganate sensitivities, DNase I cutting patterns, and loop stabilities, we conclude that linear DNA segments of short-to-medium chain length (50–180 bp) give rise to loops with the extended form of LacR and that loops formed within negatively supercoiled plasmids induce the V-shaped structure. PMID:16785444

Anisotropy of magnetic susceptibility versus lattice- and shape-preferred orientation in the Lac Tio hemo-ilmenite ore body (Grenville province, Quebec)

NASA Astrophysics Data System (ADS)

Bolle, Olivier; Charlier, Bernard; Bascou, Jérôme; Diot, Hervé; McEnroe, Suzanne A.

2014-08-01

The Lac Tio hemo-ilmenite ore body crops out in the outer portion of the 1.06 Ga Lac Allard anorthosite, a member of the Havre-Saint-Pierre anorthosite suite from the Grenville province of North America. It is made up of ilmenitite (commonly with more than 95% hemo-ilmenite) associated with noritic lithologies and anorthosite. The present study compares the magnetic fabric of the ore body, as deduced from anisotropy of magnetic susceptibility (AMS) measurements, with the crystallographic and shape fabrics, obtained from lattice-preferred orientation (LPO) and shape-preferred orientation (SPO) measurements made using electron backscattered diffraction (EBSD) and 3D image analysis, respectively. Room-temperature hysteresis measurements, thermomagnetic curves and values of the bulk magnetic susceptibility reveal a magnetic mineralogy dominated by a mixed contribution of hemo-ilmenite and magnetite. The hemo-ilmenite grains display a LPO characterized by a strong preferred orientation of the basal (0001) plane of ilmenite along which hematite was exsolved. This LPO and the magnetic fabric fit well (angle between the crystallographic c-axis and the axis of minimum susceptibility ≤ ca. 15° for most samples), and the latter is thus strongly influenced by the hemo-ilmenite magneto-crystalline anisotropy. A magnetite SPO, concordant with the hemo-ilmenite LPO, may also influence and even dominate the magnetic fabric. The rock shape fabric is coaxial with the magnetic fabric that can thus be used to perform detailed structural mapping. Interpretation of the magnetic fabric and field structural data suggests that the Lac Tio ore body would be a sag point at the margin of the Lac Allard anorthosite, deformed by ballooning during the final stage of diapiric emplacement of the anorthosite body.

Low Level Exposure to Sulfur Mustard: Development of a SOP for Analysis of Albumin Adducts and of a System for Non-Invasive Diagnosis on Skin

DTIC Science & Technology

2004-12-01

were primarily responsible for the organic chemistry and the analytical chemistry, and to Dr. F . Bikker, Mrs. Roos Mars and Mrs. Helma van Dijk for...study as hosts for bacteriophages: TG1 [K-12 A(lac-pro) supE thi hsdD5/ F ’ traD36proA+ B+ laclq lacZ AM 15] and HB2151 [K-12 ara A(lac-pro) thi/ F ’ proA+ B...TG1 [K-12 A(lac-pro) supE thi hsdD5/ F ’ traD36 proA+ B+ lacPq lacZ AM15] and HB2151 [K-12 ara A(lac-pro) thi/ F ’ proA+ B+ laclq Z AM15]. Both strains were

Adaptive Evolution of the Streptococcus pyogenes Regulatory Aldolase LacD.1

PubMed Central

Cusumano, Zachary

2013-01-01

In the human-pathogenic bacterium Streptococcus pyogenes, the tagatose bisphosphate aldolase LacD.1 likely originated through a gene duplication event and was adapted to a role as a metabolic sensor for regulation of virulence gene transcription. Although LacD.1 retains enzymatic activity, its ancestral metabolic function resides in the LacD.2 aldolase, which is required for the catabolism of galactose. In this study, we compared these paralogous proteins to identify characteristics correlated with divergence and novel function. Surprisingly, despite the fact that these proteins have identical active sites and 82% similarity in amino acid sequence, LacD.1 was less efficient at cleaving both fructose and tagatose bisphosphates. Analysis of kinetic properties revealed that LacD.1's adaptation was associated with a decrease in kcat and an increase in Km. Construction and analysis of enzyme chimeras indicated that non-active-site residues previously associated with the variable activities of human aldolase isoenzymes modulated LacD.1's affinity for substrate. Mutant LacD.1 proteins engineered to have LacD.2-like levels of enzymatic efficiency lost the ability to function as regulators, suggesting that an alteration in efficiency was required for adaptation. In competition under growth conditions that mimic a deep-tissue environment, LacD.1 conferred a significant gain in fitness that was associated with its regulatory activity. Taken together, these data suggest that LacD.1's adaptation represents a form of neofunctionalization in which duplication facilitated the gain of regulatory function important for growth in tissue and pathogenesis. PMID:23316044

A chemometric approach for optimizing protein covalent immobilization on magnetic core-shell nanoparticles in view of an alternative immunoassay.

PubMed

Teste, Bruno; Vial, Jérôme; Descroix, Stéphanie; Georgelin, Thomas; Siaugue, Jean-Michel; Petr, Jan; Varenne, Anne; Hennion, Marie-Claire

2010-06-15

A chemometric approach was developed to optimize the grafting of a bovine milk allergen: alpha-Lactalbumin (alpha-Lac) on colloidal functionalized magnetic core-shell nanoparticles (MCSNP). Such nanoparticles, functionalized with polyethyleneglycol and amino groups, exhibit a 30nm physical diameter and behave as a quasi-homogeneous system. The alpha-Lac immobilization was achieved through the covalent binding between MCSNP amino groups and alpha-Lac carboxylic moieties using the well-known tandem carbodiimide (EDC) and hydroxysulfosuccinimide (NHS). In this study, a chemometric approach was employed to highlight the parameters influencing the number of grafted proteins on the MCSNP. Three factors were evaluated: the ratio in concentration between EDC and alpha-Lac, between NHS and EDC and the concentration of alpha-Lac. After a first full factorial design to delimit the region of the space where the optimum could be located, a central composite design was then carried out to predict the best grafting conditions. It was established and experimentally confirmed that the optimum parameters are [EDC]/[alpha-Lac]=25; [NHS]/[EDC]=1.55 and alpha-Lac=24.85nmolmL(-1). In these optimal conditions, MCSNP surface was successfully saturated with alpha-Lac (34 alpha-Lac/MCSNP) with a high reproducibility (RSD=2%). The colloidal stability of MCSNP grafted with alpha-Lac as well as the immunological interactions using anti alpha-Lac antibody were then investigated in different buffers. The results emphasized that a 50mM MES buffer (pH 6) allows an efficient immune capture and a satisfying colloidal stability which provide an immunological interaction in homogeneous liquid phase.

Design and application of a lactulose biosensor.

PubMed

Wu, Jieyuan; Jiang, Peixia; Chen, Wei; Xiong, Dandan; Huang, Linglan; Jia, Junying; Chen, Yuanyuan; Jin, Jian-Ming; Tang, Shuang-Yan

2017-04-07

In this study the repressor of Escherichia coli lac operon, LacI, has been engineered for altered effector specificity. A LacI saturation mutagenesis library was subjected to Fluorescence Activated Cell Sorting (FACS) dual screening. Mutant LacI-L5 was selected and it is specifically induced by lactulose but not by other disaccharides tested (lactose, epilactose, maltose, sucrose, cellobiose and melibiose). LacI-L5 has been successfully used to construct a whole-cell lactulose biosensor which was then applied in directed evolution of cellobiose 2-epimerase (C2E) for elevated lactulose production. The mutant C2E enzyme with ~32-fold enhanced expression level was selected, demonstrating the high efficiency of the lactulose biosensor. LacI-L5 can also be used as a novel regulatory tool. This work explores the potential of engineering LacI for customized molecular biosensors which can be applied in practice.

Evaluation of coloring efficacy of lac dye in comminuted meat product.

PubMed

Divya; Singh, R P; Baboo, B; Prasad, K M

2011-06-01

Effect of incorporation of graded levels (4, 6, 8, 10, 25 ppm) of lac dye on coloring efficacy and possible use of this natural color in processed meat products was studied. Inclusion of lac dye at different concentrations did not affect the pH significantly whereas a linear increase in the Lovibond red color unit of chicken nuggets was noted with raising the level of lac dye from 4 to 10 ppm. The sensory rating for color was highest at addition level of 25 ppm of lac dye and it was comparable to color score of the product containing 200 ppm sodium nitrite. Lac dye inclusion in nuggets at all concentrations studied had better antimicrobial properties as compared to 200 ppm sodium nitrite. It was concluded that lac dye from 10 to 25 ppm could be incorporated in comminuted meat products as a natural colorant with antimicrobial action.

The business of refractive laser assisted cataract surgery (ReLACS).

PubMed

Berdahl, John P; Jensen, Matthew P

2014-01-01

Refractive Laser Assisted Cataract Surgery (ReLACS) combines the femtosecond laser with other noncovered tests and services in an attempt to reduce spectacle dependence in combination with cataract surgery. Significant interest is present among ophthalmologists who are considering adopting this technology, however significant capital outlays and continuing expenses can make the decision to adopt ReLACS foreboding. We review the financial considerations of ReLACS and review the trends seen in early adopters of this technology. Recent findings have shown that ReLACS is a growing segment of cataract surgery. Most practices who have implemented the technology have broken even and have a positive outlook on the financial return of implementing the ReLACS program. The average break-even analysis point for practices is around 230 cases a year. ReLACS is growing and appears to be a financial viable approach for many practices.

Regulation and Adaptive Evolution of Lactose Operon Expression in Lactobacillus delbrueckii

PubMed Central

Lapierre, Luciane; Mollet, Beat; Germond, Jacques-Edouard

2002-01-01

Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis are both used in the dairy industry as homofermentative lactic acid bacteria in the production of fermented milk products. After selective pressure for the fast fermentation of milk in the manufacture of yogurts, L. delbrueckii subsp. bulgaricus loses its ability to regulate lac operon expression. A series of mutations led to the constitutive expression of the lac genes. A complex of insertion sequence (IS) elements (ISL4 inside ISL5), inserted at the border of the lac promoter, induced the loss of the palindromic structure of one of the operators likely involved in the binding of regulatory factors. A lac repressor gene was discovered downstream of the β-galactosidase gene of L. delbrueckii subsp. lactis and was shown to be inactivated by several mutations in L. delbrueckii subsp. bulgaricus. Regulatory mechanisms of the lac gene expression of L. delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis were compared by heterologous expression in Lactococcus lactis of the two lac promoters in front of a reporter gene (β-glucuronidase) in the presence or absence of the lac repressor gene. Insertion of the complex of IS elements in the lac promoter of L. delbrueckii subsp. bulgaricus increased the promoter's activity but did not prevent repressor binding; rather, it increased the affinity of the repressor for the promoter. Inactivation of the lac repressor by mutations was then necessary to induce the constitutive expression of the lac genes in L. delbrueckii subsp. bulgaricus. PMID:11807052

Evidence that selected amplification of a bacterial lac frameshift allele stimulates Lac(+) reversion (adaptive mutation) with or without general hypermutability.

PubMed Central

Slechta, E Susan; Liu, Jing; Andersson, Dan I; Roth, John R

2002-01-01

In the genetic system of Cairns and Foster, a nongrowing population of an E. coli lac frameshift mutant appears to specifically accumulate Lac(+) revertants when starved on medium including lactose (adaptive mutation). This behavior has been attributed to stress-induced general mutagenesis in a subpopulation of starved cells (the hypermutable state model). We have suggested that, on the contrary, stress has no direct effect on mutability but favors only growth of cells that amplify their leaky mutant lac region (the amplification mutagenesis model). Selection enhances reversion primarily by increasing the mutant lac copy number within each developing clone on the selection plate. The observed general mutagenesis is attributed to a side effect of growth with an amplification-induction of SOS by DNA fragments released from a tandem array of lac copies. Here we show that the S. enterica version of the Cairns system shows SOS-dependent general mutagenesis and behaves in every way like the original E. coli system. In both systems, lac revertants are mutagenized during selection. Eliminating the 35-fold increase in mutation rate reduces revertant number only 2- to 4-fold. This discrepancy is due to continued growth of amplification cells until some clones manage to revert without mutagenesis solely by increasing their lac copy number. Reversion in the absence of mutagenesis is still dependent on RecA function, as expected if it depends on lac amplification (a recombination-dependent process). These observations support the amplification mutagenesis model. PMID:12136002

Antisense RNA: effect of ribosome binding sites, target location, size, and concentration on the translation of specific mRNA molecules.

PubMed

Daugherty, B L; Hotta, K; Kumar, C; Ahn, Y H; Zhu, J D; Pestka, S

1989-01-01

A series of plasmids were constructed to generate RNA complementary to the beta-galactosidase messenger RNA under control of the phage lambda PL promoter. These plasmids generate anti-lacZ mRNA bearing or lacking a synthetic ribosome binding site adjacent to the lambda PL promoter and/or the lacZ ribosome binding site in reverse orientation. Fragments of lacZ DNA from the 5' and/or the 3' region were used in these constructions. When these anti-mRNA molecules were produced in Escherichia coli 294, maximal inhibition of beta-galactosidase synthesis occurred when a functional ribosome binding site was present near the 5' end of the anti-mRNA and the anti-mRNA synthesized was complementary to the 5' region of the mRNA corresponding to the lacZ ribosome binding site and/or the 5'-coding sequence. Anti-mRNAs producing maximal inhibition of beta-galactosidase synthesis exhibited an anti-lacZ mRNA:normal lacZ mRNA ratio of 100:1 or higher. Those showing lower levels of inhibition exhibited much lower anti-lacZ mRNA:normal lacZ mRNA ratios. A functional ribosome binding site at the 5'-end was found to decrease the decay rate of the anti-lacZ mRNAs. In addition, the incorporation of a transcription terminator just downstream of the antisense segment provided for more efficient inhibition of lacZ mRNA translation due to synthesis of smaller and more abundant anti-lacZ mRNAs. The optimal constructions produced undetectable levels of beta-galactosidase synthesis.

Proteins mediating DNA loops effectively block transcription.

PubMed

Vörös, Zsuzsanna; Yan, Yan; Kovari, Daniel T; Finzi, Laura; Dunlap, David

2017-07-01

Loops are ubiquitous topological elements formed when proteins simultaneously bind to two noncontiguous DNA sites. While a loop-mediating protein may regulate initiation at a promoter, the presence of the protein at the other site may be an obstacle for RNA polymerases (RNAP) transcribing a different gene. To test whether a DNA loop alters the extent to which a protein blocks transcription, the lac repressor (LacI) was used. The outcome of in vitro transcription along templates containing two LacI operators separated by 400 bp in the presence of LacI concentrations that produced both looped and unlooped molecules was visualized with scanning force microscopy (SFM). An analysis of transcription elongation complexes, moving for 60 s at an average of 10 nt/s on unlooped DNA templates, revealed that they more often surpassed LacI bound to the lower affinity O2 operator than to the highest affinity Os operator. However, this difference was abrogated in looped DNA molecules where LacI became a strong roadblock independently of the affinity of the operator. Recordings of transcription elongation complexes, using magnetic tweezers, confirmed that they halted for several minutes upon encountering a LacI bound to a single operator. The average pause lifetime is compatible with RNAP waiting for LacI dissociation, however, the LacI open conformation visualized in the SFM images also suggests that LacI could straddle RNAP to let it pass. Independently of the mechanism by which RNAP bypasses the LacI roadblock, the data indicate that an obstacle with looped topology more effectively interferes with transcription. © 2017 The Authors Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society.

Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa.

PubMed

Kraatz, Mareike; Wallace, R John; Svensson, Liselott

2011-04-01

Strain A2 is an anaerobic, variably Gram-stain-positive, non-spore-forming, small and irregularly rod-shaped bacterium from the ruminal fluid of a sheep that has been described informally as a representative of 'Olsenella (basonym Atopobium) oviles'. Three phenotypically similar bacterial strains (lac15, lac16 and lac31(T)) were isolated in concert with Veillonella magna lac18(T) from the mucosal jejunum of a pig. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strains A2, lac15, lac16 and lac31(T) formed a genetically coherent group (100 % interstrain sequence similarity) within the bigeneric Olsenella-Atopobium branch of the family Coriobacteriaceae, class Actinobacteria. This group was most closely related to the type strains of the two recognized Olsenella species, namely Olsenella uli (sequence similarity of 96.85 %) and Olsenella profusa (sequence similarity of 97.20 %). The sequence similarity to the type strain of Atopobium minutum, the type species of the genus Atopobium, was 92.33 %. Unlike those of O. uli and O. profusa, outgrown colonies of strains A2, lac15, lac16 and lac31(T) were opaque and greyish-white with an umbonate elevation on solid culture media. The four novel strains were characterized as being well-adapted and presumably indigenous to the gastrointestinal tract of homoeothermic vertebrates: they were mesophilic, microaerotolerant, neutrophilic and acidotolerant, bile-resistant, mucin-utilizing and markedly peptidolytic lactic acid bacteria. The results of DNA-DNA hybridizations, cellular fatty acid analysis and other differential phenotypic (physiological and biochemical) tests confirmed that strains A2, lac15, lac16 and lac31(T) represent a novel species of the genus Olsenella. On the basis of the genotypic and phenotypic results, we therefore describe Olsenella umbonata sp. nov., with lac31(T) ( = CCUG 58604(T)  = DSM 22620(T)  = JCM 16156(T)) as the type strain and A2 ( = CCUG 58212  = DSM 22619  = JCM 16157) as an additionally available reference strain. Also, based on our data, we propose emended descriptions of the genus Olsenella and the species Olsenella uli and Olsenella profusa.

Extraordinary Activity in the BL Lacertae Object OJ 287

NASA Astrophysics Data System (ADS)

Hughes, Philip A.; Aller, Hugh D.; Aller, Margo F.

1998-08-01

We use a continuous wavelet transform to analyze more than two decades of data for the BL Lac object OJ 287 acquired as part of the University of Michigan Radio Astronomy Observatory (UMRAO) variability program. We find clear evidence for a persistent modulation of the total flux and polarization with period ~1.66 yr and for another signal that dominates activity in the 1980s with period ~1.12 yr. The relationship between these two variations can be understood in terms of a ``shock-in-jet'' model, in which the longer timescale periodicity is associated with an otherwise quiescent jet and the shorter timescale activity is associated with the passage of a shock. The different periodicities of these two components may reflect different internal conditions of the two flow domains leading to different wave speeds or different contractions of a single underlying periodicity due to the different Doppler factors of the two flow components. We suggest that the modulation arises from a wave driven by some asymmetric disturbance close to the central engine. The periodic behavior in polarization exhibits excursions in U that correspond to a direction ~45° from the VLBI jet axis. This behavior is not explained by the random walk in the Q-U plane that is expected from models in which a pattern of randomly aligned magnetic field elements propagate across the visible portion of the flow and suggests a small amplitude, cyclic variation in the flow direction in that part of the flow that dominates centimeter wavelength emission.

Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction: comparison with defined substrate and plating methods for water quality monitoring.

PubMed Central

Bej, A K; McCarty, S C; Atlas, R M

1991-01-01

Multiplex polymerase chain reaction (PCR) and gene probe detection of target lacZ and uidA genes were used to detect total coliform bacteria and Escherichia coli, respectively, for determining water quality. In tests of environmental water samples, the lacZ PCR method gave results statistically equivalent to those of the plate count and defined substrate methods accepted by the U.S. Environmental Protection Agency for water quality monitoring and the uidA PCR method was more sensitive than 4-methylumbelliferyl-beta-D-glucuronide-based defined substrate tests for specific detection of E. coli. Images PMID:1768116

Lateral Attitude Change.

PubMed

Glaser, Tina; Dickel, Nina; Liersch, Benjamin; Rees, Jonas; Süssenbach, Philipp; Bohner, Gerd

2015-08-01

The authors propose a framework distinguishing two types of lateral attitude change (LAC): (a) generalization effects, where attitude change toward a focal object transfers to related objects, and (b) displacement effects, where only related attitudes change but the focal attitude does not change. They bring together examples of LAC from various domains of research, outline the conditions and underlying processes of each type of LAC, and develop a theoretical framework that enables researchers to study LAC more systematically in the future. Compared with established theories of attitude change, the LAC framework focuses on lateral instead of focal attitude change and encompasses both generalization and displacement. Novel predictions and designs for studying LAC are presented. © 2014 by the Society for Personality and Social Psychology, Inc.

Mouse alpha1(I)-collagen promoter is the best known promoter to drive efficient Cre recombinase expression in osteoblast.

PubMed

Dacquin, Romain; Starbuck, Michael; Schinke, Thorsten; Karsenty, Gérard

2002-06-01

Cell- and time-specific gene inactivation should enhance our knowledge of bone biology. Implementation of this technique requires construction of transgenic mouse lines expressing Cre recombinase in osteoblasts, the bone forming cell. We tested several promoter fragments for their ability to drive efficient Cre expression in osteoblasts. In the first mouse transgenic line, the Cre gene was placed under the control of the 2.3-kb proximal fragment of the alpha1(I)-collagen promoter, which is expressed at high levels in osteoblasts throughout their differentiation. Transgenic mice expressing this transgene in bone were bred with the ROSA26 reporter (R26R) strain in which the ROSA26 locus is targeted with a conditional LacZ reporter cassette. In R26R mice, Cre expression and subsequent Cre-mediated recombination lead to expression of the LacZ reporter gene, an event that can be monitored by LacZ staining. LacZ staining was detected in virtually all osteoblasts of alpha1(I)-Cre;R26R mice indicating that homologous recombination occurred in these cells. No other cell type stained blue. In the second line studied, the 1.3-kb fragment of osteocalcin gene 2 (OG2) promoter, which is active in differentiated osteoblasts, was used to drive Cre expression. OG2-Cre mice expressed Cre specifically in bone. However, cross of OG2-Cre mice with R26R mice did not lead to any detectable LacZ staining in osteoblasts. Lastly, we tested a more active artificial promoter derived from the OG2 promoter. The artificial OG2-Cre transgene was expressed by reverse transcriptase-polymerase chain reaction in cartilage and bone samples. After cross of the artificial OG2-Cre mice with R26R mice, we detected a LacZ staining in articular chondrocytes but not in osteoblasts. Our data suggest that the only promoter able to drive Cre expression at a level sufficient to induce recombination in osteoblasts is the alpha1(I)-collagen promoter. Copyright 2002 Wiley-Liss, Inc.

Targeted retrograde transfection of adenovirus vector carrying brain-derived neurotrophic factor gene prevents loss of mouse (twy/twy) anterior horn neurons in vivo sustaining mechanical compression.

PubMed

Xu, Kan; Uchida, Kenzo; Nakajima, Hideaki; Kobayashi, Shigeru; Baba, Hisatoshi

2006-08-01

Immunohistochemical analysis after adenovirus (AdV)-mediated BDNF gene transfer in and around the area of mechanical compression in the cervical spinal cord of the hyperostotic mouse (twy/twy). To investigate the neuroprotective effect of targeted AdV-BDNF gene transfection in the twy mouse with spontaneous chronic compression of the spinal cord motoneurons. Several studies reported the neuroprotective effects of neurotrophins on injured spinal cord. However, no report has described the effect of targeted retrograde neurotrophic gene delivery on motoneuron survival in chronic compression lesions of the cervical spinal cord resembling lesions of myelopathy. LacZ marker gene using adenoviral vector (AdV-LacZ) was used to evaluate retrograde delivery from the sternomastoid muscle in adult twy mice (16-week-old) and (control). Four weeks after the AdV-LacZ or AdV-BDNF injection, the compressed cervical spinal cord was removed en bloc for immunohistologic investigation of b-galactosidase activity and immunoreactivity and immunoblot analyses of BDNF. The number of anterior horn neurons was counted using Nissl, ChAT and AChE staining. Spinal accessory motoneurons between C1 and C3 segments were successfully transfected by AdV-LacZ in both twy and ICR mice after targeted intramuscular injection. Immunoreactivity to BDNF was significantly stronger in AdV-BDNF-gene transfected twy mice than in AdV-LacZ-gene transfected mice. At the cord level showing the maximum compression in AdV-BDNF-transfected twy mice, the number of anterior horn neurons was sinificantly higher in the topographic neuronal cell counting of Nissl-, ChAT-, and AChE-stained samples than in AdV-LacZ-injected twy mice. Targeted AdV-BDNF-gene delivery significantly increased Nissl-stained anterior horn neurons and enhanced cholinergic enzyme activities in the twy. Our results suggest that targeted retrograde AdV-BDNF-gene in vivo delivery may enhance neuronal survival even under chronic mechanical compression.

Regulation of lac Operon Expression: Reappraisal of the Theory of Catabolite Repression

PubMed Central

Wanner, Barry L.; Kodaira, Ryoji; Neidhardt, Frederick C.

1978-01-01

The physiological state of Escherichia coli with respect to (permanent) catabolite repression was assessed by measuring the steady-state level of β-galactosidase in induced or in constitutive cells under a variety of growth conditions. Four results were obtained. (i) Catabolite repression had a major effect on fully induced or constitutive expression of the lac gene, and the magnitude of this effect was found to be dependent on the promoter structure; cells with a wild-type lac promoter showed an 18-fold variation in lac expression, and cells with the lacP37 (formerly lac-L37) promoter exhibited several hundred-fold variation. (ii) Exogenous adenosine cyclic 3′,5′-monophosphoric acid (cAMP) could not abolish catabolite repression, even though several controls demonstrated that cAMP was entering the cells in significant amounts. (Rapid intracellular degradation of cAMP could not be ruled out.) (iii) Neither the growth rate nor the presence of biosynthetic products altered the degree of catabolite repression; all variation could be related to the catabolites present in the growth medium. (iv) Slowing by imposing an amino acid restriction decreased the differential rate of β-galactosidase synthesis from the wild-type lac promoter when bacteria were cultured in either the absence or presence of cAMP; this decreased lac expression also occurred when the bacteria harbored the catabolite-insensitive lacP5 (formerly lacUV5) promoter mutation. These findings support the idea that (permanent) catabolite repression is set by the catabolites in the growth medium and may not be related to an imbalance between catabolism and anabolism. PMID:214424

Data Collection: A Cybernetic Aspect of a Learning Assistance Center.

ERIC Educational Resources Information Center

Devirian, Margaret Coda

Data collection and analysis as a cybernetic aspect of a Learning Assistance Center (LAC) is discussed. Using the LAC at California State University Long Beach (CSULB) as a model, the LAC is defined as a support, delivery, and referral service for the entire campus community. A LAC is held accountable to itself and its users through a cybernetics…

The Health Role of Local Area Coordinators in Scotland: A Mixed Methods Study

ERIC Educational Resources Information Center

Brown, Michael; Karatzias, Thanos; O'Leary, Lisa

2013-01-01

The study set out to explore whether local area coordinators (LACs) and their managers view the health role of LACs as an essential component of their work and identify the health-related activities undertaken by LACs in Scotland. A mixed methods cross-sectional phenomenological study involving local authority service managers (n = 25) and LACs (n…

Increased circular RNA hsa_circ_0012673 acts as a sponge of miR-22 to promote lung adenocarcinoma proliferation.

PubMed

Wang, Xiyong; Zhu, Xiaoli; Zhang, Hongming; Wei, Shuzhen; Chen, Yan; Chen, Yang; Wang, Fei; Fan, Xiaobo; Han, Shuhua; Wu, Guoqiu

2018-02-19

Recent reports have indicated that circular RNA (circRNA) may regulate Lung adenocarcinoma (LAC) development. Our previous studies showed that hsa_circ_0012673 was up-regulated in a circRNA microarray. However, its expression level in LAC has not been verified, and the underlying molecular mechanisms in LAC are unknown. In this study, we found that the expression of hsa_circ_0012673 was up-regulated in LAC tissues compared to pair-matched adjacent non-tumor tissues (P = 0.0079), and that the expression level was associated with tumour size (P = 0.015). Furthermore, hsa_circ_0012673 was primarily localized in the cytoplasm and promoted cell proliferation of LAC cells by sponging miR-22, which targeted erb-b2 receptor tyrosine kinase 3 (ErbB3) in LAC. Hsa_circ_0012673 promotes LAC proliferation by suppressing miR-22, which targets ErbB3. Copyright © 2018 Elsevier Inc. All rights reserved.

Enhanced phenol removal in an innovative lignite activated coke-assisted biological process.

PubMed

Zhang, Chen; Li, Jianfeng; Cheng, Fangqin; Liu, Yu

2018-07-01

In this study, a lignite activated coke (LAC)-assisted activated sludge (AS) process was developed for enhancing biodegradation of phenol, while the effects of LAC on sludge properties and microbial community structure were investigated. It was found that more than 90% of phenol was removed within 1 h in the LAC/AS, which was 3 times higher than the conventional AS process. Moreover, the floc size and settleability were also significantly improved in the LAC/AS. These results suggested that LAC could serve as the nucleating agent to promote the formation of compact floc, which was beneficial for toxicity mitigation and system stability. The microbial community analysis by 16S high-throughput pyrosequencing technology further revealed a more abundant bacterial richness and diversity in the LAC/AS process loaded with phenol, while some phenol degraders, such as Propionibacteriaceae were enriched. Engineering implications further suggests the LAC-assisted AS process is technically sound and economically viable. Copyright © 2018 Elsevier Ltd. All rights reserved.

Synchrotron and Synchrotron Self-Compton Spectral Signatures and Blazar Emission Models

NASA Technical Reports Server (NTRS)

Chiang, James; Boettcher, Markus; White, Nicholas E. (Technical Monitor)

2002-01-01

We find that energy losses due to synchrotron self-Compton (BBC) emission in Blazar jets can produce distinctive signatures in the time-averaged synchrotron and SSC spectra of these objects. For a fairly broad range of particle injection distributions, SSC-loss-dominated synchrotron emission exhibits a spectral dependence Fv approximately v (exp -3/2). The presence or absence of this dependence in the optical and ultraviolet spectra of flat-spectrum radio quasars such as PC 279 and in the soft X-ray spectra of high-frequency BL Lac objects such as Mark 501 gives a robust measure of the importance of SSC losses. Furthermore, for partially cooled particle distributions, spectral breaks of varying sizes can appear in the synchrotron and SSC spectra and will be related to the spectral indices of the emission below the break. These spectral signatures place constraints on the size scale and the nonthermal particle content of the emitting plasma, as well as the observer orientation relative to the jet axis.

An RXTE Study of M87 and the Core of the Virgo Cluster

NASA Technical Reports Server (NTRS)

Reynolds, Christopher S.; Heinz, Sebastian; Fabian, Andrew C.; Begelman, Mitchell C.

1998-01-01

We present hard X-ray observations of the nearby radio galaxy M87 and the core of the Virgo cluster using the Rossi X-ray Timing Explorer. These are the first hard X-ray observations of M87 not affected by contamination from the nearby Seyfert 2 galaxy NGC 4388. Thermal emission from Virgo's intracluster medium is clearly detected and has a spectrum indicative of kT approx. = 2.5 keV plasma with approximately 25% cosmic abundances. No non-thermal (power-law) emission from M87 is detected in the hard X-ray band, with fluctuations in the Cosmic X-ray Background being the limiting factor. Combining with ROSAT data, we infer that the X-ray spectrum of the M87 core and jet must be steep (Gamma(sub core) greater than 1.90 and Gamma(sub jet) greater than 1.75), and we discuss the implications of this result. In particular, these results are consistent with M87 being a mis-aligned BL-Lac object.

Development of a spectral break in the nonthermal emission of AO 0235+164

NASA Technical Reports Server (NTRS)

Odell, S. L.; Puschell, J. J.; Stein, W. A.; Warner, J. W.

1977-01-01

Results are reported for braod-band photometry of the BL Lac object AO 0235+164 carried out over the spectral range from 0.36 to 3.5 microns in December 1975 and in October and November 1976. It is found that the continuum of this object steepened at visible wavelengths between December 1975 and October 1976, but maintained a relatively constant slope in the near-IR despite a factor-of-ten decrease in flux from the maximum level. Visible-wavelength data are cited which appear to suggest that the steepening of the visible spectrum occurred in less than 300 days, while the near-IR level may have been nearly constant. It is shown that the observed change in the spectral shape of the visible-wavelength continuum cannot be explained in terms of a 'composite' model consisting of an unchanging galaxy and a varying nonthermal source with constant spectral index. Two other general classes of models are considered: intrinsic variability and extrinsic modulation.

An RXTE Study of M87 and the Core of the Virgo Cluster

NASA Technical Reports Server (NTRS)

Reynolds, Christopher S.; Heinz, Sebastian; Fabian, Andrew C.; Begelman, Mitchell C.

1998-01-01

We present hard X-ray observations of the nearby radio galaxy M87 and the core of the Virgo cluster using the Rossi X-ray 7Tming Explorer. These are the first hard X-ray observations of M87 not affected by contamination from the nearby Seyfert 2 galaxy NGC 4388. Thermal emission from Virgo's intracluster medium is clearly detected and has a spectrum indicative of kT is approximately equal to 2.5 keV plasma with approximately 25% cosmic abundances. No non-thermal (power-law) emission from M87 is detected in the hard X-ray band, with fluctuations in the Cosmic X-ray Background being the limiting factor. Combining with ROSAT data, we infer that the X-ray spectrum of the M87 core and jet must be steep (Gamma (sub core) > 1.90 and Gamma (sub jet) > 1.75), and we discuss the implications of this result. In particular, these results are consistent with M87 being a mis-aligned BL-Lac object.

VizieR Online Data Catalog: Gamma-ray bright blazars spectrophotometry (Williamson+, 2014)

NASA Astrophysics Data System (ADS)

Williamson, K. E.; Jorstad, S. G.; Marscher, A. P.; Larionov, V. M.; Smith, P. S.; Agudo, I.; Arkharov, A. A.; Blinov, D. A.; Casadio, C.; Efimova, N. V.; Gomez, J. L.; Hagen-Thorn, V. A.; Joshi, M.; Konstantinova, T. S.; Kopatskaya, E. N.; Larionova, E. G.; Larionova, L. V.; Malmrose, M. P.; McHardy, I. M.; Molina, S. N.; Morozova, D. A.; Schmidt, G. D.; Taylor, B. W.; Troitsky, I. S.

2017-03-01

Since 2007, we have been collecting multi-waveband fluxes, polarization measurements, and radio images of blazars to provide the data for understanding the physics of the jets (see, e.g., Marscher 2012, arXiv:1201.5402). This study includes 28 of the original 30 objects selected for the monitoring campaign, confirmed as γ-ray sources by EGRET (Energetic γ-Ray Experiment Telescope) on the Compton Gamma Ray Observatory, have an R-band brightness exceeding 18 mag (bright enough for optical polarization measurements at a 1-2 m class optical telescope without needing excessive amounts of telescope time), exceed 0.5 Jy at 43 GHz, and have a declination accessible to the collaboration's observatories (> - 30°). Three additional BL Lacs (1055+018, 1308+326, and 1749+096) and two FSRQs (3C345 and 3C446) included in this analysis were among those added when they were detected as γ-ray sources by the Fermi LAT (Abdo et al. 2009, J/ApJ/700/597). (4 data files).

Medium-resolution far-ultraviolet spectroscopy of PKS 2155-304

NASA Technical Reports Server (NTRS)

Appenzeller, I.; Mandel, H.; Krautter, J.; Bowyer, S.; Hurwitz, M.; Grewing, M.; Kramer, G.; Kappelmann, N.

1995-01-01

Using the Berkeley spectrometer of the Orbiting Retrievable Far and Extreme Ultraviolet Spectrometer (ORFEUS) we observed the 87-117 nm UV spectrum of the BL Lac object PKS 2155-304 with about 0.5 A resolution. In addition to the expected interstellar lines we detected higher quantum number counterparts of the intergalactic Lyman alpha lines discovered earlier with IUE and the Hubble Space Telescope (HST) in the direction of PKS 2155-304. The Lyman discontinuities indicate for three of the redshifted clouds a combined H I column density of 2-5 x 10(exp 16)/sq cm, while the column density for another cloud appears to be well below 5 x 10(exp 15)/sq cm. No siginificant O VI absorption in the galactic halo toward PKS 2155-304 could be detected from our data. Assuming that saturation effects are negligible for these weak features, we obtain for the O VI column density toward PKS 2155-304 a 3 sigma upper limit of 2.7 x 10(exp 14)/sq cm.

S5 0716+714: GeV variability study

DOE PAGES

Rani, B.; Krichbaum, T. P.; Lott, B.; ...

2013-02-19

The GeV observations by Fermi-LAT give us the opportunity to characterize the high-energy emission (100 MeV–300 GeV) variability properties of the BL Lac object S5 0716+714. In this study, we performed flux and spectral analysis of more than 3 year long (August 2008 to April 2012) Fermi-LAT data of the source. During this period, the source exhibits two different modes of flux variability with characteristic timescales of ~75 and ~140 days, respectively. In addition, we also notice that the flux variations are characterized by a weak spectral hardening. The GeV spectrum of the source shows a clear deviation from amore » simple power law, and is better explained by a broken power law. Similar to other bright Fermi blazars, the break energy does not vary with the source flux during the different activity states. Finally, we discuss several possible scenarios to explain the observed spectral break.« less

S5 0716+714: GeV variability study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rani, B.; Krichbaum, T. P.; Lott, B.

The GeV observations by Fermi-LAT give us the opportunity to characterize the high-energy emission (100 MeV–300 GeV) variability properties of the BL Lac object S5 0716+714. In this study, we performed flux and spectral analysis of more than 3 year long (August 2008 to April 2012) Fermi-LAT data of the source. During this period, the source exhibits two different modes of flux variability with characteristic timescales of ~75 and ~140 days, respectively. In addition, we also notice that the flux variations are characterized by a weak spectral hardening. The GeV spectrum of the source shows a clear deviation from amore » simple power law, and is better explained by a broken power law. Similar to other bright Fermi blazars, the break energy does not vary with the source flux during the different activity states. Finally, we discuss several possible scenarios to explain the observed spectral break.« less

Results from the energetic gamma-ray experiment telescope (EGRET) on the Compton Observatory

NASA Technical Reports Server (NTRS)

Fichtel, C. E.; Bertsch, D. L.; Dingus, B.; Hartman, R. C.; Hunter, S. D.; Kanbach, G.; Kniffen, D. A.; Kwok, P. W.; Lin, Y. C.; Mattox, J. R.

1993-01-01

The Energetic Gamma-Ray Experiment Telescope (EGRET) on the Compton Gamma Ray Observatory (CGRO) covers the high energy gamma ray energy range, approximately 30 MeV to 30 GeV, with a sensitivity considerably greater than earlier high energy gamma-ray satellites. Thus far, 4 pulsars have been detected and their properties measured, including in 3 cases the energy spectrum as a function of phase. The details of the galactic plane are being mapped and a spectra of the center region has been obtained in good agreement with that expected from cosmic ray interactions. The Magellanic clouds have been examined with the Large Magellanic Cloud (LMC) having been detected at a level consistent with it having a cosmic ray density compatible with quasi-stable equilibrium. Sixteen Active Galactic Nuclei (AGN's) have been seen thus far with a high degree of certainty including 12 quasars and 4 BL Lac objects, but no Seyferts. Time variation has been detected in some of these AGN's

Louisiana SIP: LAC 33:III Ch 21 Subchap J, 2147--Limiting Volatile Organic Compound (VOC) Emissions from Reactor Processes and Distillation Operations in Synthetic Organic Chemical manufacturing Industry (SOCMI); SIP effective 1998-02-02 (LAc74) to more..

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch 21 Subchap J, 2147--Limiting Volatile Organic Compound (VOC) Emissions from Reactor Processes and Distillation Operations in Synthetic Organic Chemical manufacturing Industry (SOCMI); SIP effective 1998-02-02 (LAc74) more...

ALMACAL IV: A catalogue of ALMA calibrator continuum observations

NASA Astrophysics Data System (ADS)

Bonato, M.; Liuzzo, E.; Giannetti, A.; Massardi, M.; De Zotti, G.; Burkutean, S.; Galluzzi, V.; Negrello, M.; Baronchelli, I.; Brand, J.; Zwaan, M. A.; Rygl, K. L. J.; Marchili, N.; Klitsch, A.; Oteo, I.

2018-05-01

We present a catalogue of ALMA flux density measurements of 754 calibrators observed between August 2012 and September 2017, for a total of 16,263 observations in different bands and epochs. The flux densities were measured reprocessing the ALMA images generated in the framework of the ALMACAL project, with a new code developed by the Italian node of the European ALMA Regional Centre. A search in the online databases yielded redshift measurements for 589 sources (˜78 per cent of the total). Almost all sources are flat-spectrum, based on their low-frequency spectral index, and have properties consistent with being blazars of different types. To illustrate the properties of the sample we show the redshift and flux density distributions as well as the distributions of the number of observations of individual sources and of time spans in the source frame for sources observed in bands 3 (84-116 GHz) and 6 (211-275 GHz). As examples of the scientific investigations allowed by the catalogue we briefly discuss the variability properties of our sources in ALMA bands 3 and 6 and the frequency spectra between the effective frequencies of these bands. We find that the median variability index steadily increases with the source-frame time lag increasing from 100 to 800 days, and that the frequency spectra of BL Lacs are significantly flatter than those of flat-spectrum radio quasars. We also show the global spectral energy distributions of our sources over 17 orders of magnitude in frequency.

Intra-night Optical Variability Monitoring of Fermi Blazars: First Results from 1.3 m J. C. Bhattacharya Telescope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paliya, Vaidehi S.; Ajello, M.; Kaur, A.

We report the first results obtained from our campaign to characterize the intra-night-optical variability (INOV) properties of Fermi detected blazars, using the observations from the recently commissioned 1.3 m J. C. Bhattacharya telescope (JCBT). During the first run, we were able to observe 17 blazars in the Bessel R filter for ∼137 hr. Using C- and scaled F -statistics, we quantify the extent of INOV and derive the duty cycle (DC), which is the fraction of time during which a source exhibits a substantial flux variability. We find a high DC of 40% for BL Lac objects and the flatmore » spectrum radio quasars are relatively less variable (DC ∼ 15%). However, when estimated for blazars sub-classes, a high DC of ∼59% is found in low synchrotron peaked (LSP) blazars, whereas, intermediate and high synchrotron peaked objects have a low DC of ∼11% and 13%, respectively. We find evidence of the association of the high amplitude INOV with the γ -ray flaring state. We also notice a high polarization during the elevated INOV states (for the sources that have polarimetric data available), thus supporting the jet based origin of the observed variability. We plan to enlarge the sample and utilize the time availability from the small telescopes, such as 1.3 m JCBT, to strengthen/verify the results obtained in this work and those existing in the literature.« less

VizieR Online Data Catalog: CRTS close supermassive black hole binaries (Graham+, 2015)

NASA Astrophysics Data System (ADS)

Graham, M. J.; Djorgovski, S. G.; Stern, D.; Drake, A. J.; Mahabal, A. A.; Donalek, C.; Glikman, E.; Larson, S.; Christensen, E.

2016-02-01

CRTS leverages the Catalina Sky Survey data streams from three telescopes - the 0.7m Catalina Sky Survey Schmidt and 1.5m Mount Lemmon Survey telescopes in Arizona, and the 0.5m Siding Springs Survey Schmidt in Australia - used in a search for Near-Earth Objects, operated by Lunar and Planetary Laboratory at University of Arizona. CRTS covers up to ~2500deg2 per night, with four exposures per visit, separated by 10-min, over 21 nights per lunation. All data are automatically processed in real-time, and optical transients are immediately distributed using a variety of electronic mechanisms. The data are broadly calibrated to Johnson V (see Drake et al., 2013, Cat. J/ApJ/763/32 for details) and the full CRTS data set contains time series for approximately 500 million sources. The Million Quasars (MQ) catalogue v3.7 contains all spectroscopically confirmed type 1 QSOs (309525), AGN (21728) and BL Lacs (1573) in the literature up to 2013 November 26 and formed the basis for the results of Graham et al. (2015Natur.518...74G). We have extended this with 297301 spectroscopically identified quasars in the SDSS Data Release 12 (Paris et al., in preparation). We cross-matched this combined quasar list against the CRTS data set with a 3-arcsec matching radius and find that 334446 confirmed quasars are covered by the full CRTS. Our final sample consists of 111 quasars. (2 data files).

Interstitial ion homeostasis and acid-base balance are maintained in oedematous brain of mice with acute toxic liver failure.

PubMed

Obara-Michlewska, Marta; Ding, Fengfei; Popek, Mariusz; Verkhratsky, Alexei; Nedergaard, Maiken; Zielinska, Magdalena; Albrecht, Jan

2018-05-14

Acute toxic liver failure (ATLF) rapidly leads to brain oedema and neurological decline. We evaluated the ability of ATLF-affected brain to control the ionic composition and acid-base balance of the interstitial fluid. ATLF was induced in 10-12 weeks old male C57Bl mice by single intraperitoneal (i.p.) injection of 100 μg/g azoxymethane (AOM). Analyses were carried out in cerebral cortex of precomatous mice 20-24 h after AOM administration. Brain fluid status was evaluated by measuring apparent diffusion coefficient [ADC] using NMR spectroscopy, Evans Blue extravasation, and accumulation of an intracisternally-injected fluorescent tracer. Extracellular pH ([pH] e ) and ([K + ] e ) were measured in situ with ion-sensitive microelectrodes. Cerebral cortical microdialysates were subjected to photometric analysis of extracellular potassium ([K + ] e ), sodium ([Na + ] e ) and luminometric assay of extracellular lactate ([Lac] e ). Potassium transport in cerebral cortical slices was measured ex vivo as 86 Rb uptake. Cerebral cortex of AOM-treated mice presented decreased ADC supporting the view that ATLF-induced brain oedema is primarily cytotoxic in nature. In addition, increased Evans blue extravasation indicated blood brain barrier leakage, and increased fluorescent tracer accumulation suggested impaired interstitial fluid passage. However, [K + ] e , [Na + ] e , [Lac] e , [pH] e and potassium transport in brain of AOM-treated mice was not different from control mice. We conclude that in spite of cytotoxic oedema and deregulated interstitial fluid passage, brain of mice with ATLF retains the ability to maintain interstitial ion homeostasis and acid-base balance. Tentatively, uncompromised brain ion homeostasis and acid-base balance may contribute to the relatively frequent brain function recovery and spontaneous survival rate in human patients with ATLF. Copyright © 2018. Published by Elsevier Ltd.

Intestinal stem cells remain viable after prolonged tissue storage

PubMed Central

Fuller, Megan K.; Faulk, Denver M.; Sundaram, Nambirajan; Mahe, Maxime M.; Stout, Kara M.; von Furstenberg, Richard J.; Smith, Brian J.; McNaughton, Kirk K.; Shroyer, Noah F.; Helmrath, Michael A.; Henning, Susan J.

2013-01-01

Intestinal stem cells (ISCs) are responsible for renewal of the epithelium both during normal homeostasis and following injury. As such they have significant therapeutic potential. However, it is unknown whether ISCs can survive tissue storage. We hypothesized that, although the majority of epithelial cells may die, ISCs would remain viable for at least 24 h at 4°C. To explore this hypothesis, jejuni of C57Bl6/J or Lgr5-LacZ mice were removed and either processed immediately or placed in phosphate buffered saline (PBS) at 4°C. Delayed isolations of epithelia were performed after 24, 30, or 48 h storage. At the light microscope level, despite extensive apoptosis of villus epithelial cells, small intestinal crypts remained morphologically intact through 30 h and ISCs were identifiable via Lgr5-LacZ positivity. Electron microscopy showed that ISCs retain high integrity through 24 h. When assessed by flow cytometry, ISCs were more resistant to degeneration than the rest of the epithelium, including neighboring Paneth cells, with higher viability across all time points. Culture of isolated crypts showed no loss of capacity to form complex enteroids after 24 h tissue storage, with efficiencies after 7 days of culture remaining above 80%. By 30 h storage, efficiencies declined but budding capability was retained. We conclude that, with delay in isolation, ISCs remain viable and retain their proliferative capacity. In contrast, the remainder of the epithelium, including the Paneth cells, exhibits degeneration and programmed cell death. If these findings are recapitulated with human tissue, storage at 4°C may offer a valuable temporal window for harvest of crypts or ISCs for therapeutic application. PMID:23820734

Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone.

PubMed

Macari, Soraia; Sharma, Lavanya A; Wyatt, Amanda; da Silva, Janine Maíra; Dias, George J; Silva, Tarcília A; Szawka, Raphael E; Grattan, David R

2018-05-01

The underlying causes of maxillary bone loss during lactation remain poorly understood. We evaluated the impact of lactation on physiological and mechanically-induced alveolar bone remodeling. Nulliparous non-lactating (N-LAC) and 21-day lactating (LAC) mice underwent mechanically-induced bone remodeling by orthodontic tooth movement (OTM). Micro-computed tomography (microCT) was performed in the maxilla, femur and vertebra. Tartrate-resistant-acid phosphatase (TRAP) and Masson's trichrome labelling was performed in the maxillary bone and gene expression was determined in the periodontal ligament. The effect of prolactin on osteoclast (OCL) and osteoblast (OBL) differentiation was also investigated in N-LAC and LAC mice. Lactation increased alveolar bone loss in the maxilla, femur and vertebra, while OTM was enhanced. The number of OCL and OBL was higher in the maxilla of LAC mice. OTM increased OCL in both groups; while OBL was increased only in N-LAC but not in LAC mice, in which cell numbers were already elevated. The alveolar bone loss during lactation was associated with increased expression of receptor activator of nuclear factor-KappaB (RANK), RANK ligand (RANKL), and osteoprotegerin (OPG) in the maxilla. OTM induced the same responses in N-LAC mice, whereas it had no further effect in LAC mice. Lactation enhanced differentiation of OCL and OBL from bone marrow cells, and prolactin recapitulated OCL differentiation in N-LAC mice. Thus, lactation increases physiological maxillary bone remodeling and OTM, and both require activation of RANK/RANKL/OPG system. These findings expand our knowledge of lactation-induced osteopenia and have possible impact on clinical practice regarding orthodontic treatments and dental implants in lactating women. Copyright © 2018 Elsevier Inc. All rights reserved.

Identification of a Dehydrogenase Required for Lactose Metabolism in Caulobacter crescentus▿ †‡

PubMed Central

Arellano, Benjamin H.; Ortiz, Janett D.; Manzano, Janet; Chen, Joseph C.

2010-01-01

Caulobacter crescentus, which thrives in freshwater environments with low nutrient levels, serves as a model system for studying bacterial cell cycle regulation and organelle development. We examined its ability to utilize lactose (i) to gain insight into the metabolic capacities of oligotrophic bacteria and (ii) to obtain an additional genetic tool for studying this model organism, aiming to eliminate the basal enzymatic activity that hydrolyzes the chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal). Using a previously isolated transposon mutant, we identified a gene, lacA, that is required for growth on lactose as the sole carbon source and for turning colonies blue in the presence of X-gal. LacA, which contains a glucose-methanol-choline (GMC) oxidoreductase domain, has homology to the flavin subunit of Pectobacterium cypripedii's gluconate dehydrogenase. Sequence comparisons indicated that two genes near lacA, lacB and lacC, encode the other subunits of the membrane-bound dehydrogenase. In addition to lactose, all three lac genes are involved in the catabolism of three other β-galactosides (lactulose, lactitol, and methyl-β-d-galactoside) and two glucosides (salicin and trehalose). Dehydrogenase assays confirmed that the lac gene products oxidize lactose, salicin, and trehalose. This enzymatic activity is inducible, and increased lac expression in the presence of lactose and salicin likely contributes to the induction. Expression of lacA also depends on the presence of the lac genes, implying that the dehydrogenase participates in induction. The involvement of a dehydrogenase suggests that degradation of lactose and other sugars in C. crescentus may resemble a proposed pathway in Agrobacterium tumefaciens. PMID:20190087

A biosensor based on Coriolopsis gallica laccase immobilized on nitrogen-doped multiwalled carbon nanotubes and graphene oxide for polyphenol detection

PubMed Central

Aguila, Sergio A; Shimomoto, David; Ipinza, Franscisco; Bedolla-Valdez, Zaira I; Romo-Herrera, José; Contreras, Oscar E; Farías, Mario H; Alonso-Núñez, Gabriel

2015-01-01

The use of nanomaterials allows the design of ultrasensitive biosensors with advantages in the detection of organic molecules. Catechol and catechin are molecules that occur naturally in fruits, and their presence in products like dyes and wines affects quality standards. In this study, catechol and catechin were measured at the nanoscale by means of cyclic voltammetry. The oxidation of Coriolopsis gallica laccase immobilized on nitrogen-doped multiwalled carbon nanotubes (Lac/CNx-MWCNT) and on graphene oxide (Lac/GO) was used to measure the concentrations of catechol and catechin. Nitrogen-doped multiwalled carbon nanotubes (CNx-MWCNT) were synthesized by spray pyrolysis and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and x-ray photoelectron spectroscopy (XPS). Covalently bonded hybrids with laccase (Lac/CNx-MWCNT and Lac/GO) were generated. Catalytic activity of free enzymes determined with syringaldazine yielded 14 584 UmL−1. With Lac/CNx-MWCNT at concentrations of 6.4 mmol L−1 activity was 9326 U mL−1, while enzyme activity measured with Lac/GO at concentration of 6.4 mmol L−1 was 9 234 U mL−1. The Lac/CNx-MWCNT hybrid showed higher stability than Lac/GO at different ethyl alcohol concentrations. The Lac/CNx-MWCNT hybrid can measure concentrations, not previously reported, as low as 1 × 10−8 mol L−1 by measuring the electric current responses. PMID:27877839

A biosensor based on Coriolopsis gallica laccase immobilized on nitrogen-doped multiwalled carbon nanotubes and graphene oxide for polyphenol detection

NASA Astrophysics Data System (ADS)

Aguila, Sergio A.; Shimomoto, David; Ipinza, Franscisco; Bedolla-Valdez, Zaira I.; Romo-Herrera, José; Contreras, Oscar E.; Farías, Mario H.; Alonso-Núñez, Gabriel

2015-10-01

The use of nanomaterials allows the design of ultrasensitive biosensors with advantages in the detection of organic molecules. Catechol and catechin are molecules that occur naturally in fruits, and their presence in products like dyes and wines affects quality standards. In this study, catechol and catechin were measured at the nanoscale by means of cyclic voltammetry. The oxidation of Coriolopsis gallica laccase immobilized on nitrogen-doped multiwalled carbon nanotubes (Lac/CNx-MWCNT) and on graphene oxide (Lac/GO) was used to measure the concentrations of catechol and catechin. Nitrogen-doped multiwalled carbon nanotubes (CNx-MWCNT) were synthesized by spray pyrolysis and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and x-ray photoelectron spectroscopy (XPS). Covalently bonded hybrids with laccase (Lac/CNx-MWCNT and Lac/GO) were generated. Catalytic activity of free enzymes determined with syringaldazine yielded 14 584 UmL-1. With Lac/CNx-MWCNT at concentrations of 6.4 mmol L-1 activity was 9326 U mL-1, while enzyme activity measured with Lac/GO at concentration of 6.4 mmol L-1 was 9 234 U mL-1. The Lac/CNx-MWCNT hybrid showed higher stability than Lac/GO at different ethyl alcohol concentrations. The Lac/CNx-MWCNT hybrid can measure concentrations, not previously reported, as low as 1 × 10-8 mol L-1 by measuring the electric current responses.

Louisiana SIP: LAC 33:III Ch. 7 Section 701. Purpose and Information Regarding Standards for PM10, SO2, CO, Atmospheric Oxidants, NOx and Pb; SIP effective 1989-05-08 (LAc49) to 2011-08-03 (LAd34 - Revised)

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch. 7 Section 701. Purpose and Information Regarding Standards for PM10, SO2, CO, Atmospheric Oxidants, NOx and Pb; SIP effective 1989-05-08 (LAc49) to 2011-08-03 (LAd34 - Revised)

K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose.

PubMed

Sarabia-Sainz, Andre-I; Sarabia-Sainz, Hector Manuel; Montfort, Gabriela Ramos-Clamont; Mata-Haro, Veronica; Guzman-Partida, Ana María; Guzman, Roberto; Garcia-Soto, Mariano; Vazquez-Moreno, Luz

2015-09-16

The formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10-17 µm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections.

K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose

PubMed Central

Sarabia-Sainz, Andre-i; Sarabia-Sainz, Hector Manuel; Ramos-Clamont Montfort, Gabriela; Mata-Haro, Veronica; Guzman-Partida, Ana María; Guzman, Roberto; Garcia-Soto, Mariano; Vazquez-Moreno, Luz

2015-01-01

The formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10–17 µm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections. PMID:26389896

Lactosylated Gramicidin-based lipid nanoparticles (Lac-GLN) for targeted delivery of anti-miR-155 to hepatocellular carcinoma

PubMed Central

Zhang, Mengzi; Zhou, Xiaoju; Wang, Bo; Yung, Bryant C.; Lee, Ly J.; Ghoshal, Kalpana; Lee, Robert J.

2013-01-01

Lactosylated gramicidin-containing lipid nanoparticles (Lac-GLN) were developed for delivery of anti-microRNA-155 (anti-miR-155) to hepatocellular carcinoma (HCC) cells. MiR-155 is an oncomiR frequently elevated in HCC. The Lac-GLN formulation contained N-lactobionyl-dioleoyl phosphatidylethanolamine (Lac-DOPE), a ligand for the asialoglycoprotein receptor (ASGR), and an antibiotic peptide gramicidin A. The nanoparticles exhibited a mean particle diameter of 73 nm, zeta potential of +3.5 mV, anti-miR encapsulation efficiency of 88%, and excellent colloidal stability at 4°C. Lac-GLN effectively delivered anti-miR-155 to HCC cells with a 16.1- and 4.1-fold up-regulation of miR-155 targets C/EBPβ and FOXP3 genes, respectively, and exhibited significant greater efficiency over Lipofectamine 2000. In mice, intravenous injection of Lac-GLN containing Cy3-anti-miR-155 led to preferential accumulation of the anti-miR-155 in hepatocytes. Intravenous administration of 1.5 mg/kg anti-miR-155 loaded Lac-GLN resulted in up-regulation of C/EBPβ and FOXP3 by 6.9- and 2.2- fold, respectively. These results suggest potential application of Lac-GLN as a liver-specific delivery vehicle for anti-miR therapy. PMID:23567045

Comparative Studies of the Proteome, Glycoproteome, and N-Glycome of Clear Cell Renal Cell Carcinoma Plasma before and after Curative Nephrectomy

PubMed Central

2015-01-01

Clear cell renal cell carcinoma is the most prevalent of all reported kidney cancer cases, and currently there are no markers for early diagnosis. This has stimulated great research interest recently because early detection of the disease can significantly improve the low survival rate. Combining the proteome, glycoproteome, and N-glycome data from clear cell renal cell carcinoma plasma has the potential of identifying candidate markers for early diagnosis and prognosis and/or to monitor disease recurrence. Here, we report on the utilization of a multi-dimensional fractionation approach (12P-M-LAC) and LC–MS/MS to comprehensively investigate clear cell renal cell carcinoma plasma collected before (disease) and after (non-disease) curative nephrectomy (n = 40). Proteins detected in the subproteomes were investigated via label-free quantification. Protein abundance analysis revealed a number of low-level proteins with significant differential expression levels in disease samples, including HSPG2, CD146, ECM1, SELL, SYNE1, and VCAM1. Importantly, we observed a strong correlation between differentially expressed proteins and clinical status of the patient. Investigation of the glycoproteome returned 13 candidate glycoproteins with significant differential M-LAC column binding. Qualitative analysis indicated that 62% of selected candidate glycoproteins showed higher levels (upregulation) in M-LAC bound fraction of disease samples. This observation was further confirmed by released N-glycans data in which 53% of identified N-glycans were present at different levels in plasma in the disease vs non-disease samples. This striking result demonstrates the potential for significant protein glycosylation alterations in clear cell renal cell carcinoma cancer plasma. With future validation in a larger cohort, information derived from this study may lead to the development of clear cell renal cell carcinoma candidate biomarkers. PMID:25184692

Multiple Ant Species Tending Lac Insect Kerria yunnanensis (Hemiptera: Kerriidae) Provide Asymmetric Protection against Parasitoids

PubMed Central

Li, Qiao; Hoffmann, Benjamin D.; Zhang, Wei

2014-01-01

This study investigated the effects of ant attendance on the parasitoid community and parasitism of lac insect Kerria yunnanensis aggregations in Yunnan province, China. We manipulated ant attendance to establish three treatments: (1) ant exclusion; (2) low ant attendance by several ant species; and (3) high ant attendance by Crematogaster macaoensis. Five parasitoid species were collected, with two species contributing 82.7 and 13.2% of total abundance respectively. Total parasitoid abundance was lowest in the February sample when K. yunnanensis was in its younger life stage, being significantly lower in the ant exclusion treatment. In April, all three treatments had significantly different parasitoid abundances, being highest in the ant exclusion treatment and the lowest in the high ant attendance treatment. When ants were present, there were strong negative relationships between total parasitoid abundance and ant abundance, with the relationships being dependent upon the ant species composition and abundance. The patterns of total parasitoid abundance were driven by the two most abundant parasitoid species. Parasitoid species richness did not differ among treatments or between sample times, however, multivariate analysis confirmed that overall parasitoid community structure differed significantly among treatments and between sample times, with the high ant attendance treatment differing most from the other two treatments. Interestingly the absence of ants did not result in increased parasitism from four of the five parasitoids. Ants in lac insect farming systems have a clear role for agricultural pest management. A full understanding of the asymmetric abilities of ants to influence parasitoid communities, and affect parasitism of hosts will require further experimental manipulation to assess the relative roles of 1) the abundance of each individual ant species on parasitoid access to hosts, 2) competition among parasitoids, and 3) the interaction between the first two factors. PMID:24887398

Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader.

PubMed

Schaefer, Jorrit; Jovanovic, Goran; Kotta-Loizou, Ioly; Buck, Martin

2016-06-15

Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ß-d-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by J. H. Miller in 1972) can be challenging for a large number of samples, is prone to variability, and involves hazardous compounds for lysis (e.g., chloroform, toluene). Here we describe a single-step assay using a 96-well microplate reader with a proven alternative cell permeabilization method. This modified protocol reduces handling time by 90%. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Laccase 1 gene from Plutella xylostella (PxLac1) and its functions in humoral immune response.

PubMed

Wang, Ze-Hua; Hu, Rong-Min; Ye, Xi-Qian; Huang, Jian-Hua; Chen, Xue-Xin; Shi, Min

Laccase (EC 1.10.3.2) is a phenoloxidase found in many insect species. The Laccase 1 gene from Plutella xylostella (PxLac1) was cloned, and its expression patterns and functions were determined using qPCR and RNAi methods. The results showed that the expression levels of PxLac1 were consistently high in all larval stages, and the most abundant was in the midgut during the 4th instar stage. Moreover, the expression of PxLac1 was up-regulated in response to bacterial infection, and decreased 24 h after being parasitized by Cotesia vestalis. Further analyses indicated that the effect of parasitization on PxLac1 was induced by active C. vestalis Bracovirus (CvBV). Haemocyte-free hemolymph phenoloxidase (PO) activity was suppressed when PxLac1 was treated with RNAi. Our results provide evidence for a connection between the Laccase 1 gene and insect immunity, and revealed that parasitoid polydnavirus suppresses host PO activity via PxLac1 regulation. Copyright © 2018. Published by Elsevier Ltd.

AlloRep: A Repository of Sequence, Structural and Mutagenesis Data for the LacI/GalR Transcription Regulators.

PubMed

Sousa, Filipa L; Parente, Daniel J; Shis, David L; Hessman, Jacob A; Chazelle, Allen; Bennett, Matthew R; Teichmann, Sarah A; Swint-Kruse, Liskin

2016-02-22

Protein families evolve functional variation by accumulating point mutations at functionally important amino acid positions. Homologs in the LacI/GalR family of transcription regulators have evolved to bind diverse DNA sequences and allosteric regulatory molecules. In addition to playing key roles in bacterial metabolism, these proteins have been widely used as a model family for benchmarking structural and functional prediction algorithms. We have collected manually curated sequence alignments for >3000 sequences, in vivo phenotypic and biochemical data for >5750 LacI/GalR mutational variants, and noncovalent residue contact networks for 65 LacI/GalR homolog structures. Using this rich data resource, we compared the noncovalent residue contact networks of the LacI/GalR subfamilies to design and experimentally validate an allosteric mutant of a synthetic LacI/GalR repressor for use in biotechnology. The AlloRep database (freely available at www.AlloRep.org) is a key resource for future evolutionary studies of LacI/GalR homologs and for benchmarking computational predictions of functional change. Copyright © 2015 Elsevier Ltd. All rights reserved.

On Three-dimensional Structures in Relativistic Hydrodynamic Jets

NASA Astrophysics Data System (ADS)

Hardee, Philip E.

2000-04-01

The appearance of wavelike helical structures on steady relativistic jets is studied using a normal mode analysis of the linearized fluid equations. Helical structures produced by the normal modes scale relative to the resonant (most unstable) wavelength and not with the absolute wavelength. The resonant wavelength of the normal modes can be less than the jet radius even on highly relativistic jets. High-pressure regions helically twisted around the jet beam may be confined close to the jet surface, penetrate deeply into the jet interior, or be confined to the jet interior. The high-pressure regions range from thin and ribbon-like to thick and tubelike depending on the mode and wavelength. The wave speeds can be significantly different at different wavelengths but are less than the flow speed. The highest wave speed for the jets studied has a Lorentz factor somewhat more than half that of the underlying flow speed. A maximum pressure fluctuation criterion found through comparison between theory and a set of relativistic axisymmetric jet simulations is applied to estimate the maximum amplitudes of the helical, elliptical, and triangular normal modes. Transverse velocity fluctuations for these asymmetric modes are up to twice the amplitude of those associated with the axisymmetric pinch mode. The maximum amplitude of jet distortions and the accompanying velocity fluctuations at, for example, the resonant wavelength decreases as the Lorentz factor increases. Long-wavelength helical surface mode and shorter wavelength helical first body mode generated structures should be the most significant. Emission from high-pressure regions as they twist around the jet beam can vary significantly as a result of angular variation in the flow direction associated with normal mode structures if they are viewed at about the beaming angle θ=1/γ. Variation in the Doppler boost factor can lead to brightness asymmetries by factors up to 6 as long-wavelength helical structure produced by the helical surface mode winds around the jet. Higher order surface modes and first body modes produce less variation. Angular variation in the flow direction associated with the helical mode appears consistent with precessing jet models that have been proposed to explain the variability in 3C 273 and BL Lac object AO 0235+164. In particular, cyclic angular variation in the flow direction produced by the normal modes could produce the activity seen in BL Lac object OJ 287. Jet precession provides a mechanism for triggering the helical modes on multiple length scales, e.g., the galactic superluminal GRO J1655-40.

Optical monitoring of BL Lac object S5 0716+714 and FSRQ 3C 273 from 2000 to 2014

NASA Astrophysics Data System (ADS)

Yuan, Yu-Hai; Fan, Jun-hui; Tao, Jun; Qian, Bo-Chen; Costantin, Denise; Xiao, Hu-Bing; Pei, Zhi-Yuan; Lin, Chao

2017-09-01

Context. Using the 1.56 m telescope at the Shanghai Observatory (ShAO), China, we monitored two sources, BL Lac object S5 0716+714 and flat spectrum radio quasar (FSRQ) 3C 273. For S5 0716+714, we report 4969 sets of CCD (Charge-coupled Device) photometrical optical observations (1369 for V band, 1861 for R band and 1739 for I band) in the monitoring time from Dec. 4, 2000 to Apr. 5, 2014. For 3C 273, we report 460 observations (138 for V band, 146 for R band and 176 for I band) in the monitoring time from Mar. 28, 2006 to Apr. 9, 2014. Aims: The observations provide us with a large amount of data to analyze the short-term and long-term optical variabilities. Based on the variable timescales, we can estimate the central black hole mass and the Doppler factor. An abundance of multi-band observations can help us to analyze the relations between the brightness and spectrum. Methods: We use Gaussian fitting to analyze the intra-day light curves and obtain the intra-day variability (IDV) timescales. We use the discrete correlation function (DCF) method and Jurkevich method to analyze the quasi-periodic variability. Based on the VRI observations, we use the linear fitting to analyze the relations between brightness and spectrum. Results: The two sources both show IDV properties for S5 0716+714. The timescales are in the range from 17.3 min to 4.82 h; for 3C 273, the timescale is ΔT = 35.6 min. Based on the periodic analysis methods, we find the periods PV = 24.24 ± 1.09 days, PR = 24.12 ± 0.76 days, PI = 24.82 ± 0.73 days for S5 0716+714, and P = 12.99 ± 0.72, 21.76 ± 1.45 yr for 3C 273. The two sources displayed the "bluer-when-brighter" spectral evolution properties. Conclusions: S5 0716+714 and 3C 273 are frequently studied objects. The violent optical variability and IDV may come from the jet. Gaussian fitting can be used to analyze IDVs. The relations between brightness (flux density) and spectrum are strongly influenced by the frequency. A table of the individual photometry measurements is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/605/A43

The preventive role of levosimendan against bleomycin-induced pulmonary fibrosis in rats.

PubMed

Gürbüzel, Mehmet; Sayar, Ilyas; Cankaya, Murat; Gürbüzel, Ahmet; Demirtas, Levent; Bakirci, Eftal Murat; Capoglu, Ilyas

2016-04-01

In this study, the effects of levosimendan used in the treatment of acute congestive heart failure upon pulmonary fibrosis in rats induced with bleomycin (BL) were analyzed. A total of 33 male Sprague-Dawley type rats were categorized into five groups randomly. About 2.5U/kg BL was intratracheally administered to the rats in the BL, BL+L1, BL+L2, and BL+L3 groups, and 0.9% saline was intratracheally administered at the same rate to the control group. 0.3, 1, and 3mg/kg levosimendan was intraperitoneally administered to the BL+L1, BL+L2, and BL+L3 groups, respectively. Blood and tissue samples were taken from the rats euthanized to determine the changes in erythrocyte enzyme activities and to conduct histopathological evaluations after 14 days. With values between 0 and 3, histopathological scoring damage was assessed by the presence of inflammation and fibrosis in a semiquantitative manner. Compared with those in the C group, glutathione reductase (GR) and Catalase (CAT) enzymes decreased in the BL group; compared with that in the BL group, GR increased in the BL+L1 and BL+L3 groups, 6-phosphogluconate dehydrogenase (6PGD) increased in the BL+L3 group, and CAT increased in the BL+L2 and BL+L3 groups (p<0.05). In the histopathological evaluation, fibrosis occurred in all rats in the BL group, and tissue damage was noticed to be generally less in the BL+L1, BL+L2, and BL+L3 groups (p<0.001). The results obtained from biochemical and histopathological evaluations indicate that levosimendan had an anti-fibrotic effect without a dose-dependent response on pulmonary fibrosis. Copyright © 2015 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Preparation of a Ammonia-Treated Lac Dye and Structure Elucidation of Its Main Component.

PubMed

Nishizaki, Yuzo; Ishizuki, Kyoko; Akiyama, Hiroshi; Tada, Atsuko; Sugimoto, Naoki; Sato, Kyoko

2016-01-01

Lac dye and cochineal extract contain laccaic acids and carminic acid as the main pigments, respectively. Both laccaic acids and carminic acid are anthraquinone derivatives. 4-Aminocarminic acid (acid-stable carmine), an illegal colorant, has been detected in several processed foods. 4-Aminocarminic acid is obtained by heating cochineal extract (carminic acid) in ammonia solution. We attempted to prepare ammonia-treated lac dye and to identify the structures of the main pigment components. Ammonia-treated lac dye showed acid stability similar to that of 4-aminocarminic acid. The structures of the main pigments in ammonia-treated lac dye were analyzed using LC/MS. One of the main pigments was isolated and identified as 4-aminolaccaic acid C using various NMR techniques, including 2D-INADEQUATE. These results indicated that ammonia-treatment of lac dye results in the generation of 4-aminolaccaic acids.

Evaluation of Polymerase Chain Reaction for Detecting Coliform Bacteria in Drinking Water Sources.

PubMed

Isfahani, Bahram Nasr; Fazeli, Hossein; Babaie, Zeinab; Poursina, Farkhondeh; Moghim, Sharareh; Rouzbahani, Meisam

2017-01-01

Coliform bacteria are used as indicator organisms for detecting fecal pollution in water. Traditional methods including microbial culture tests in lactose-containing media and enzyme-based tests for the detection of β-galactosidase; however, these methods are time-consuming and less specific. The aim of this study was to evaluate polymerase chain reaction (PCR) for detecting coliform. Totally, 100 of water samples from Isfahan drinking water source were collected. Coliform bacteria and Escherichia coli were detected in drinking water using LacZ and LamB genes in PCR method performed in comparison with biochemical tests for all samples. Using phenotyping, 80 coliform isolates were found. The results of the biochemical tests illustrated 78.7% coliform bacteria and 21.2% E. coli . PCR results for LacZ and LamB genes were 67.5% and 17.5%, respectively. The PCR method was shown to be an effective, sensitive, and rapid method for detecting coliform and E. coli in drinking water from the Isfahan drinking water sources.

X-ray study of a sample of FR0 radio galaxies: unveiling the nature of the central engine

NASA Astrophysics Data System (ADS)

Torresi, E.; Grandi, P.; Capetti, A.; Baldi, R. D.; Giovannini, G.

2018-06-01

Fanaroff-Riley type 0 radio galaxies (FR0s) are compact radio sources that represent the bulk of the radio-loud active galactic nuclei (AGN) population, but they are still poorly understood. Pilot studies on these sources have been already performed at radio and optical wavelengths: here we present the first X-ray study of a sample of 19 FR0 radio galaxies selected from the Sloan Digital Sky Survey/NRAO VLA Sky Survey/Faint Images of the Radio Sky at Twenty-cm sample of Best & Heckman, with redshift ≤0.15, radio size ≤10 kpc, and optically classified as low-excitation galaxies. The X-ray spectra are modelled with a power-law component absorbed by Galactic column density with, in some cases, a contribution from thermal extended gas. The X-ray photons are likely produced by the jet as attested by the observed correlation between X-ray (2-10 keV) and radio (5 GHz) luminosities, similar to Fanaroff-Riley type I radio galaxies (FRIs). The estimated Eddington-scaled luminosities indicate a low accretion rate. Overall, we find that the X-ray properties of FR0s are indistinguishable from those of FRIs, thus adding another similarity between AGN associated with compact and extended radio sources. A comparison between FR0s and low-luminosity BL Lacs rules out important beaming effects in the X-ray emission of the compact radio galaxies. FR0s have different X-ray properties with respect to young radio sources (e.g. gigahertz-peaked spectrum/compact steep spectrum sources), generally characterized by higher X-ray luminosities and more complex spectra. In conclusion, the paucity of extended radio emission in FR0s is probably related to the intrinsic properties of their jets that prevent the formation of extended structures, and/or to intermittent activity of their engines.

Black Hole Demographics in and Nuclear Properties of Nearby Low Luminosity Radio Galaxies; Connections to Radio Activity?

NASA Technical Reports Server (NTRS)

Baum, S. A.; Kleijn, G. A. Verdoes; Xu, C.; ODea, C. P.; deZeeuw, P. T.

2004-01-01

We combine the results of an HST STIS and WFPC study of a complete sample of 21 nearby UGC low luminosity radio galaxies with the results of a radio VLA and VLBA study of the same sample. We examine the relationship between the stellar and gaseous properties of the galaxies on tens to hundreds of parsec scale with the properties of the radio jets on the same scale. From the VLA and VLBA data we constrain the physics of the outflowing radio plasma from the tens of parsecs to hundreds of kiloparsec scales. From the WFPC2 H alpha and dust images and the STIS kinematics of the near nuclear gas we obtain constraints on the orientation of near nuclear disks of gas and measures of the nuclear stellar, continuum point source, and line emission fluxes. Under the statistically supported assumption that the radio jet issues perpendicular to the disk, we use the orientation of the optical (large scale accretion?) disks to constrain the three-dimensional orientation of the radio ejection. From HST/STIS spectroscopy of the near-nuclear emission line gas we obtain measures/limits on the black hole masses. We examine correlations between the VLBA and VLA-scale radio emission, the nuclear line emission, and the nuclear optical and radio continuum emission. Though our sample is relatively small, it is uniquely well defined, spans a narrow range in redshift and we have a consistent set of high resolution data with which to carefully examine these relationships. We use the combined radio and optical data to: 1) Constrain the orientation, physics, and bulk outflow speed of the radio plasma; 2) Put limits on the mass accretion rate and study the relationship between black hole mass, radio luminosity, and near nuclear gaseous content; 3) Provide insight into the relationship between BL Lac objects and low luminosity radio galaxies.

Optical Variability Properties of High Luminosity AGN Classes

NASA Astrophysics Data System (ADS)

Stalin, C. S.; Gopal Krishna; Sagar, Ram; Wiita, Paul J.

2004-03-01

We present the results of a comparative study of the intranight optical variability (INOV) characteristics of radio-loud and radioquiet quasars, which involves a systematic intra-night optical monitoring of seven sets of high luminosity AGNs covering the redshift range z ' 0:2 to z ' 2:2. The sample, matched in the optical luminosity - redshift .MB?z/ plane, consists of seven radio-quiet quasars (RQQs), eight radio lobedominated quasars (LDQs), five radio core-dominated quasars (CDQs) and six BL Lac objects (BLs). Systematic CCD observations, aided by a careful data analysis procedure, have allowed us to detect INOV with amplitudes as low as about 1%. Present observations cover a total of 113 nights (720 hours) with only a single quasar monitored as continuously as possible on a given night. Considering the cases of only unambiguous detections of INOV we have estimated duty cycles (DCs) of 17%, 12%, 20% and 61% for RQQs, LDQs, CDQs, and BLs, respectively. The much lower amplitude and DC of INOV shown by RQQs compared to BLs may be understood in terms of their having optical synchrotron jets which are modestly misdirected from us. From our fairly extensive dataset, no general trend of a correlation between the INOVamplitude and the apparent optical brightness of the quasar is noticed. This suggests that the physical mechanisms of INOV and long term optical variability (LTOV) do not have a one-to-one relationship and different factors are involved. Also, the absence of a clear negative correlation between the INOV and LTOV characteristics of blazars of our sample points toward an inconspicuous contribution of accretion disk fluctuations to the observed INOV. The INOVduty cycle of theAGNs observed in this program suggests that INOV is associated predominantly with the highly polarized optical emission components. We also report new VLA imaging of two RQQs .1029C329&1252C020/ in our sample which has yielded a 5 GHz detection in one of them .1252 C 020I S5 GHz ' 1 mJy/.

Comparative genome analysis of Streptococcus infantarius subsp. infantarius CJ18, an African fermented camel milk isolate with adaptations to dairy environment.

PubMed

Jans, Christoph; Follador, Rainer; Hochstrasser, Mira; Lacroix, Christophe; Meile, Leo; Stevens, Marc J A

2013-03-22

Streptococcus infantarius subsp. infantarius (Sii) belongs to the Streptococcus bovis/Streptococcus equinus complex associated with several human and animal infections. Sii is a predominant bacterium in spontaneously fermented milk products in Africa. The genome sequence of Sii strain CJ18 was compared with that of other Streptococcus species to identify dairy adaptations including genome decay such as in Streptococcus thermophilus, traits for its competitiveness in spontaneous milk fermentation and to assess potential health risks for consumers. The genome of Sii CJ18 harbors several unique regions in comparison to Sii ATCC BAA-102T, among others an enlarged exo- and capsular polysaccharide operon; Streptococcus thermophilus-associated genes; a region containing metabolic and hypothetical genes mostly unique to CJ18 and the dairy isolate Streptococcus gallolyticus subsp. macedonicus; and a second oligopeptide transport operon. Dairy adaptations in CJ18 are reflected by a high percentage of pseudogenes (4.9%) representing genome decay which includes the inactivation of the lactose phosphotransferase system (lacIIABC) by multiple transposases integration. The presence of lacS and lacZ genes is the major dairy adaptation affecting lactose metabolism pathways also due to the disruption of lacIIABC.We constructed mutant strains of lacS, lacZ and lacIIABC and analyzed the resulting strains of CJ18 to confirm the redirection of lactose metabolism via LacS and LacZ.Natural competence genes are conserved in both Sii strains, but CJ18 contains a lower number of CRISPR spacers which indicates a reduced defense capability against alien DNA. No classical streptococcal virulence factors were detected in both Sii strains apart from those involved in adhesion which should be considered niche factors. Sii-specific virulence factors are not described. Several Sii-specific regions encoding uncharacterized proteins provide new leads for virulence analyses and investigation of the unclear association of dairy and clinical Sii with human diseases. The genome of the African dairy isolate Sii CJ18 clearly differs from the human isolate ATCC BAA-102T. CJ18 possesses a high natural competence predisposition likely explaining the enlarged genome. Metabolic adaptations to the dairy environment are evident and especially lactose uptake corresponds to S. thermophilus. Genome decay is not as advanced as in S. thermophilus (10-19%) possibly due to a shorter history in dairy fermentations.

Comparative genome analysis of Streptococcus infantarius subsp. infantarius CJ18, an African fermented camel milk isolate with adaptations to dairy environment

PubMed Central

2013-01-01

Background Streptococcus infantarius subsp. infantarius (Sii) belongs to the Streptococcus bovis/Streptococcus equinus complex associated with several human and animal infections. Sii is a predominant bacterium in spontaneously fermented milk products in Africa. The genome sequence of Sii strain CJ18 was compared with that of other Streptococcus species to identify dairy adaptations including genome decay such as in Streptococcus thermophilus, traits for its competitiveness in spontaneous milk fermentation and to assess potential health risks for consumers. Results The genome of Sii CJ18 harbors several unique regions in comparison to Sii ATCC BAA-102T, among others an enlarged exo- and capsular polysaccharide operon; Streptococcus thermophilus-associated genes; a region containing metabolic and hypothetical genes mostly unique to CJ18 and the dairy isolate Streptococcus gallolyticus subsp. macedonicus; and a second oligopeptide transport operon. Dairy adaptations in CJ18 are reflected by a high percentage of pseudogenes (4.9%) representing genome decay which includes the inactivation of the lactose phosphotransferase system (lacIIABC) by multiple transposases integration. The presence of lacS and lacZ genes is the major dairy adaptation affecting lactose metabolism pathways also due to the disruption of lacIIABC. We constructed mutant strains of lacS, lacZ and lacIIABC and analyzed the resulting strains of CJ18 to confirm the redirection of lactose metabolism via LacS and LacZ. Natural competence genes are conserved in both Sii strains, but CJ18 contains a lower number of CRISPR spacers which indicates a reduced defense capability against alien DNA. No classical streptococcal virulence factors were detected in both Sii strains apart from those involved in adhesion which should be considered niche factors. Sii-specific virulence factors are not described. Several Sii-specific regions encoding uncharacterized proteins provide new leads for virulence analyses and investigation of the unclear association of dairy and clinical Sii with human diseases. Conclusions The genome of the African dairy isolate Sii CJ18 clearly differs from the human isolate ATCC BAA-102T. CJ18 possesses a high natural competence predisposition likely explaining the enlarged genome. Metabolic adaptations to the dairy environment are evident and especially lactose uptake corresponds to S. thermophilus. Genome decay is not as advanced as in S. thermophilus (10-19%) possibly due to a shorter history in dairy fermentations. PMID:23521820

Louisiana SIP: LAC 33:III Ch 23 Subchap B, §2303--Aluminum Plants, §2303 Standards for Horizontal Stud Soderberg Primary Aluminum Plants and Prebake Primary Aluminum Plants; SIP effective 1989-05-08 (LAc49) to 2011-08-03 (LAad34 - Revised)

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch 23 Subchap B, §2303--Aluminum Plants, §2303 Standards for Horizontal Stud Soderberg Primary Aluminum Plants and Prebake Primary Aluminum Plants; SIP effective 1989-05-08 (LAc49) to 2011-08-03 (LAad34 - Revised)

Plasmid linkage of the D-tagatose 6-phosphate pathway in Streptococcus lactis: effect on lactose and galactose metabolism.

PubMed Central

Crow, V L; Davey, G P; Pearce, L E; Thomas, T D

1983-01-01

The three enzymes of the D-tagatose 6-phosphate pathway (galactose 6-phosphate isomerase, D-tagatose 6-phosphate kinase, and tagatose 1,6-diphosphate aldolase) were absent in lactose-negative (Lac-) derivatives of Streptococcus lactis C10, H1, and 133 grown on galactose. The lactose phosphoenolpyruvate-dependent phosphotransferase system and phospho-beta-galactosidase activities were also absent in Lac- derivatives of strains H1 and 133 and were low (possibly absent) in C10 Lac-. In all three Lac- derivatives, low galactose phosphotransferase system activity was found. On galactose, Lac- derivatives grew more slowly (presumably using the Leloir pathway) than the wild-type strains and accumulated high intracellular concentrations of galactose 6-phosphate (up to 49 mM); no intracellular tagatose 1,6-diphosphate was detected. The data suggest that the Lac phenotype is plasmid linked in the three strains studied, with the evidence being more substantial for strain H1. A Lac- derivative of H1 contained a single plasmid (33 megadaltons) which was absent from the Lac- mutant. We suggest that the genes linked to the lactose plasmid in S. lactis are more numerous than previously envisaged, coding for all of the enzymes involved in lactose metabolism from initial transport to the formation of triose phosphates via the D-tagatose 6-phosphate pathway. Images PMID:6294064

agr-Dependent Interactions of Staphylococcus aureus USA300 with Human Polymorphonuclear Neutrophils

PubMed Central

Pang, Yun Yun; Schwartz, Jamie; Thoendel, Matthew; Ackermann, Laynez W.; Horswill, Alexander R.; Nauseef, William M.

2010-01-01

The emergence of serious infections due to community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has fueled interest in the contributions of specific staphylococcal virulence factors to clinical disease. To assess the contributions of agr-dependent factors to the fate of organisms in polymorphonuclear neutrophils (PMN), we examined the consequences for organism and host cells of feeding PMN with wild-type CA-MRSA (LAC) or CA-MRSA (LAC agr KO) at different multiplicities of infection (MOIs). Phagocytosed organisms rapidly increased the transcription of RNAIII in a time- and MOI-dependent fashion; extracellular USA300 (LAC) did not increase RNAIII expression despite having the capacity to respond to autoinducing peptide-enriched culture medium. HOCl-mediated damage and intracellular survival were the same in the wild-type and USA300 (LAC agr KO). PMN lysis by ingested USA300 (LAC) was time- and MOI-dependent and, at MOIs >1, required α-hemolysin (hla) as USA300 (LAC agr KO) and USA300 (LAC hla KO) promoted PMN lysis only at high MOIs. Taken together, these data demonstrate activation of the agr operon in human PMN with the subsequent production of α-hemolysin and PMN lysis. The extent to which these events in the phagosomes of human PMN contribute to the increased morbidity and mortality of infections with USA300 (LAC) merits further study. PMID:20829608

LACCASE Is Necessary and Nonredundant with PEROXIDASE for Lignin Polymerization during Vascular Development in Arabidopsis[C][W

PubMed Central

Zhao, Qiao; Nakashima, Jin; Chen, Fang; Yin, Yanbin; Fu, Chunxiang; Yun, Jianfei; Shao, Hui; Wang, Xiaoqiang; Wang, Zeng-Yu; Dixon, Richard A.

2013-01-01

The evolution of lignin biosynthesis was critical in the transition of plants from an aquatic to an upright terrestrial lifestyle. Lignin is assembled by oxidative polymerization of two major monomers, coniferyl alcohol and sinapyl alcohol. Although two recently discovered laccases, LAC4 and LAC17, have been shown to play a role in lignin polymerization in Arabidopsis thaliana, disruption of both genes only leads to a relatively small change in lignin content and only under continuous illumination. Simultaneous disruption of LAC11 along with LAC4 and LAC17 causes severe plant growth arrest, narrower root diameter, indehiscent anthers, and vascular development arrest with lack of lignification. Genome-wide transcript analysis revealed that all the putative lignin peroxidase genes are expressed at normal levels or even higher in the laccase triple mutant, suggesting that lignin laccase activity is necessary and nonredundant with peroxidase activity for monolignol polymerization during plant vascular development. Interestingly, even though lignin deposition in roots is almost completely abolished in the lac11 lac4 lac17 triple mutant, the Casparian strip, which is lignified through the activity of peroxidase, is still functional. Phylogenetic analysis revealed that lignin laccase genes have no orthologs in lower plant species, suggesting that the monolignol laccase genes diverged after the evolution of seed plants. PMID:24143805

Effect of sample preparation and preenrichment media on the recovery of Salmonella from cantaloupes, mangoes, and tomatoes.

PubMed

Hammack, Thomas S; Johnson, Mildred L; Jacobson, Andrew P; Andrews, Wallace H

2006-01-01

Studies were conducted to determine the relative effectiveness of buffered peptone water (BPW), lactose (LAC) broth, and Universal Preenrichment (UP) broth for the recovery of Salmonella organisms from fruit rinses, whole fruit, and comminuted fruit. In the first phase, the relative effectiveness of the rinse and soak methods for the recovery of Salmonella from surface-contaminated mangoes and tomatoes was examined. Fruits were spot inoculated with single Salmonella serovars and held for 4 days at 2-6 degrees C before analysis was initiated. The contaminated fruit was rinsed in portions of BPW, LAC broth, or UP broth. Portions from each rinse were added to its respective broth (e.g., BPW to BPW). Individual whole fruit, in their remaining broth rinses (soak method), and the fruit rinse/broths (rinse method) were incubated for 24 h at 35 degrees C. The Bacteriological Analytical Manual (BAM) Salmonella culture method was followed thereafter. The soak method produced significantly greater numbers (P < 0.05) of positive test portions than did the rinse method for the analysis of mangoes (93 versus 12) and tomatoes (85 versus 34). The 3 broths were comparable for the recovery of Salmonella for both the soak and the rinse methods for mangoes. For tomatoes, there were no significant differences among the broths for the soak method, but BPW and UP broth were significantly more productive (P < 0.05) than LAC broth by the rinse method. In the second phase, the relative effectiveness of LAC broth, BPW, and UP broth for the recovery of Salmonella from comminuted fruit was examined. Fruits were contaminated with single Salmonella serovars and aged for 4 days at 2-6 degrees C. Twenty 25 g test portions were preenriched in each of the following broths: BPW, LAC broth, and UP broth. The BAM Salmonella culture method was followed thereafter. For cantaloupes, significantly more (P < 0.05) Salmonella-positive test portions were recovered with UP broth (96 Salmonella-positive test portions) and BPW (87 Salmonella-positive test portions) than with LAC broth (57 Salmonella-positive test portions). For mangoes, BPW recovered an arithmetically larger number of Salmonella-positive test portions (27 Salmonella-positive test portions) than did either LAC broth (14 Salmonella-positive test portions) or UP broth (18 Salmonella-positive test portions). For tomatoes, there were no significant differences among the broths: BPW recovered 65 Salmonella-positive test portions, UP broth recovered 62 Salmonella-positive test portions, and LAC broth recovered 60 Salmonella-positive test portions. For the analysis of whole fruit, it is recommended that the soak method be used. For whole fruit analyzed with the soak method, UP broth should be used for tomatoes and BPW should be used for mangoes. It is further recommended that UP broth be used for the analysis of comminuted cantaloupes and that BPW be used for the analysis of comminuted mangoes and tomatoes.

Poly-LacNAc as an Age-Specific Ligand for Rotavirus P[11] in Neonates and Infants

PubMed Central

Liu, Yang; Huang, Pengwei; Jiang, Baoming; Tan, Ming; Morrow, Ardythe L.; Jiang, Xi

2013-01-01

Rotavirus (RV) P[11] is an unique genotype that infects neonates. The mechanism of such age-specific host restriction remains unknown. In this study, we explored host mucosal glycans as a potential age-specific factor for attachment of P[11] RVs. Using in vitro binding assays, we demonstrated that VP8* of a P[11] RV (N155) could bind saliva of infants (60.3%, N = 151) but not of adults (0%, N = 48), with a significantly negative correlation between binding of VP8* and ages of infants (P<0.01). Recognition to the infant saliva did not correlate with the ABO, secretor and Lewis histo-blood group antigens (HBGAs) but with the binding of the lectin Lycopersicon esculentum (LEA) that is known to recognize the oligomers of N-acetyllactosamine (LacNAc), a precursor of human HBGAs. Direct evidence of LacNAc involvement in P[11] binding was obtained from specific binding of VP8* with homopolymers of LacNAc in variable lengths through a glycan array analysis of 611 glycans. These results were confirmed by strong binding of VP8* to the Lec2 cell line that expresses LacNAc oligomers but not to the Lec8 cell line lacking the LacNAc. In addition, N155 VP8* and authentic P[11] RVs (human 116E and bovine B223) hemagglutinated human red blood cells that are known to express poly-LacNAc. The potential role of poly-LacNAc in host attachment and infection of RVs has been obtained by abrogation of 116E replication by the PAA-conjugated poly-LacNAc, human milk, and LEA positive infant saliva. Overall, our results suggested that the poly-LacNAc could serve as an age-specific receptor for P[11] RVs and well explained the epidemiology that P[11] RVs mainly infect neonates and young children. PMID:24244290

The evaluation of acute physiology and chronic health evaluation II score, poisoning severity score, sequential organ failure assessment score combine with lactate to assess the prognosis of the patients with acute organophosphate pesticide poisoning.

PubMed

Yuan, Shaoxin; Gao, Yusong; Ji, Wenqing; Song, Junshuai; Mei, Xue

2018-05-01

The aim of this study was to assess the ability of acute physiology and chronic health evaluation II (APACHE II) score, poisoning severity score (PSS) as well as sequential organ failure assessment (SOFA) score combining with lactate (Lac) to predict mortality in the Emergency Department (ED) patients who were poisoned with organophosphate.A retrospective review of 59 stands-compliant patients was carried out. Receiver operating characteristic (ROC) curves were constructed based on the APACHE II score, PSS, SOFA score with or without Lac, respectively, and the areas under the ROC curve (AUCs) were determined to assess predictive value. According to SOFA-Lac (a combination of SOFA and Lac) classification standard, acute organophosphate pesticide poisoning (AOPP) patients were divided into low-risk and high-risk groups. Then mortality rates were compared between risk levels.Between survivors and non-survivors, there were significant differences in the APACHE II score, PSS, SOFA score, and Lac (all P < .05). The AUCs of the APACHE II score, PSS, and SOFA score were 0.876, 0.811, and 0.837, respectively. However, after combining with Lac, the AUCs were 0.922, 0.878, and 0.956, respectively. According to SOFA-Lac, the mortality of high-risk group was significantly higher than low-risk group (P < .05) and the patients of the non-survival group were all at high risk.These data suggest the APACHE II score, PSS, SOFA score can all predict the prognosis of AOPP patients. For its simplicity and objectivity, the SOFA score is a superior predictor. Lac significantly improved the predictive abilities of the 3 scoring systems, especially for the SOFA score. The SOFA-Lac system effectively distinguished the high-risk group from the low-risk group. Therefore, the SOFA-Lac system is significantly better at predicting mortality in AOPP patients.

A diverse range of bacterial and eukaryotic chitinases hydrolyzes the LacNAc (Galβ1-4GlcNAc) and LacdiNAc (GalNAcβ1-4GlcNAc) motifs found on vertebrate and insect cells.

PubMed

Frederiksen, Rikki F; Yoshimura, Yayoi; Storgaard, Birgit G; Paspaliari, Dafni K; Petersen, Bent O; Chen, Kowa; Larsen, Tanja; Duus, Jens Ø; Ingmer, Hanne; Bovin, Nicolai V; Westerlind, Ulrika; Blixt, Ola; Palcic, Monica M; Leisner, Jørgen J

2015-02-27

There is emerging evidence that chitinases have additional functions beyond degrading environmental chitin, such as involvement in innate and acquired immune responses, tissue remodeling, fibrosis, and serving as virulence factors of bacterial pathogens. We have recently shown that both the human chitotriosidase and a chitinase from Salmonella enterica serovar Typhimurium hydrolyze LacNAc from Galβ1-4GlcNAcβ-tetramethylrhodamine (LacNAc-TMR (Galβ1-4GlcNAcβ(CH2)8CONH(CH2)2NHCO-TMR)), a fluorescently labeled model substrate for glycans found in mammals. In this study we have examined the binding affinities of the Salmonella chitinase by carbohydrate microarray screening and found that it binds to a range of compounds, including five that contain LacNAc structures. We have further examined the hydrolytic specificity of this enzyme and chitinases from Sodalis glossinidius and Polysphondylium pallidum, which are phylogenetically related to the Salmonella chitinase, as well as unrelated chitinases from Listeria monocytogenes using the fluorescently labeled substrate analogs LacdiNAc-TMR (GalNAcβ1-4GlcNAcβ-TMR), LacNAc-TMR, and LacNAcβ1-6LacNAcβ-TMR. We found that all chitinases examined hydrolyzed LacdiNAc from the TMR aglycone to various degrees, whereas they were less active toward LacNAc-TMR conjugates. LacdiNAc is found in the mammalian glycome and is a common motif in invertebrate glycans. This substrate specificity was evident for chitinases of different phylogenetic origins. Three of the chitinases also hydrolyzed the β1-6 bond in LacNAcβ1-6LacNAcβ-TMR, an activity that is of potential importance in relation to mammalian glycans. The enzymatic affinities for these mammalian-like structures suggest additional functional roles of chitinases beyond chitin hydrolysis. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Prevalence of ligamentum arteriosum calcification on multi-section spiral CT and digital radiography.

PubMed

Hong, Gil-Sun; Goo, Hyun Woo; Song, Jae-Woo

2012-06-01

To investigate the prevalence of ligamentum arteriosum calcification (LAC) on multi-section spiral CT and digital radiography. Five hundred and eight children and 232 adults who performed multi-section chest CT were included in this study and were divided into nine age groups: A (0-5 years), B (6-10 years), C (11-15 years), D (16-20 years), E (21-30 years), F (31-40 years), G (41-50 years), H (51-60 years), and I (61-70 years). Two radiologists assessed the presence of LAC on axial and coronal CT images, defined as focal calcific density on both or on one plane with attenuation >100 Hounsfield unit. The prevalence of LAC on CT was compared between children and adults, and between unenhanced and enhanced CT in children. The prevalence of LAC on digital radiography was evaluated in 476 children. The prevalence of definite LAC on unenhanced multi-section CT was significantly higher in children (37.8 %) than in adults (11.2 %) (P < 0.001), with prevalences in groups: A through I of 35.8, 48.7, 35.1, 28.6, 25.0, 10.2, 15.5, 7.8, and 5.6 %, respectively. The prevalences of indeterminate LAC in age groups A-I on unenhanced multi-section CT were 4.5, 12.8, 8.1, 19.0, 0.0, 0.0, 0.0, 2.0, and 1.9 %. In children, the prevalence of LAC was significantly higher on unenhanced than on enhanced CT (37.8 vs. 16.4 %, P < 0.001). The prevalence of LAC on digital radiography was 3.6 % in children. LAC is frequently observed in children and adults on multi-section spiral CT, more frequently than previously reported. Compared with that on multi-section spiral CT, the prevalence of LAC on digital radiography is substantially low.

A portable bioluminescence engineered cell-based biosensor for on-site applications.

PubMed

Roda, Aldo; Cevenini, Luca; Michelini, Elisa; Branchini, Bruce R

2011-04-15

We have developed a portable biosensing device based on genetically engineered bioluminescent (BL) cells. Cells were immobilized on a 4 × 3 multiwell cartridge using a new biocompatible matrix that preserved their vitality. Using a fiber optic taper, the cartridge was placed in direct contact with a cooled CCD sensor to image and quantify the BL signals. Yeast and bacterial cells were engineered to express recognition elements, whose interaction with the analyte led to luciferase expression, via reporter gene technology. Three different biosensors were developed. The first detects androgenic compounds using yeast cells carrying a green-emitting P. pyralis luciferase regulated by the human androgen receptor and a red mutant of the same species as internal vitality control. The second biosensor detects two classes of compounds (androgens and estrogens) using yeast strains engineered to express green-or red-emitting mutant firefly luciferases in response to androgens or estrogens, respectively. The third biosensor detects lactose analogue isopropyl β-d-1-thiogalactopyranoside using two E. coli strains. One strain exploits the lac operon as recognition element for the expression of P. pyralis luciferase. The other strain serves as a vitality control expressing Gaussia princeps luciferase, which requires a different luciferin substrate. The immobilized cells were stable for up to 1 month. The analytes could be detected at nanomolar levels with good precision and accuracy when the specific signal was corrected using the internal vitality control. This portable device can be used for on-site multiplexed bioassays for different compound classes. Copyright © 2011 Elsevier B.V. All rights reserved.

Louisiana SIP: LAC 33:III Ch 61 Subchap A, §6121 to § 6131--Method 43 - Capture Efficiency Test Procedures; SIP effective 1994-06-06 (LAc60) to to 2011-08-03 (LAd34 - Moved to Chap 21 Subchap N §§ 2155-2160 and revised)

EPA Pesticide Factsheets

Louisiana SIP: LAC 33:III Ch 61 Subchap A, §6121 to § 6131--Method 43 - Capture Efficiency Test Procedures; SIP effective 1994-06-06 (LAc60) to to 2011-08-03 (LAd34 - Moved to Chap 21 Subchap N §§ 2155-2160 and revised)

Latent class evaluation of a milk test, a urine test, and the fat-to-protein percentage ratio in milk to diagnose ketosis in dairy cows.

PubMed

Krogh, M A; Toft, N; Enevoldsen, C

2011-05-01

In this study, 3 commonly used tests to diagnose ketosis were evaluated with a latent class model to avoid the assumption of an available perfect test. The 3 tests were the KetoLac BHB (Sanwa Kagaku Kenkyusho Co. Ltd., Nagoya, Japan) test strip that tests milk for β-hydroxybutyrate, the KetoStix (Bayer Diagnostics Europe Ltd., Dublin, Ireland) test strip that tests urine for acetoacetate, and the fat-to-protein percentage ratio (FPR) in milk. A total of 8,902 cows were included in the analysis. The cows were considered to be a random sample from the population of Danish dairy cattle under intensive management, thus representing a natural spectrum of ketosis as a disease. All cows had a recorded FPR between 7 and 21 d postpartum. The KetoLac BHB recordings were available from 2,257 cows and 6,645 cows had a KetoStix recording. The recordings were analyzed with a modified Hui-Walter model, in a Bayesian framework. The specificity of the KetoLac BHB test and the KetoStix test were both high [0.99 (0.97-0.99)], whereas the specificity of FPR was somewhat lower [0.79 (0.77-0.81)]. The best sensitivity was for the KetoStix test [0.78 (0.55-0.98)], followed by the FPR [0.63 (0.58-0.71)] and KetoLac BHB test [0.58 (0.35-0.93)]. Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Major Cardiovascular Risk Factors in Latin America: A Comparison with the United States. The Latin American Consortium of Studies in Obesity (LASO)

PubMed Central

Chirinos, Julio A.; Gómez, Luis F.; Perel, Pablo; Pichardo, Rafael; González, Angel; Sánchez, José R.; Ferreccio, Catterina; Aguilera, Ximena; Silva, Eglé; Oróstegui, Myriam; Medina-Lezama, Josefina; Pérez, Cynthia M.; Suárez, Erick; Ortiz, Ana P.; Rosero, Luis; Schapochnik, Noberto; Ortiz, Zulma; Ferrante, Daniel; Casas, Juan P.

2013-01-01

Background Limited knowledge on the prevalence and distribution of risk factors impairs the planning and implementation of cardiovascular prevention programs in the Latin American and Caribbean (LAC) region. Methods and Findings Prevalence of hypertension, diabetes mellitus, abnormal lipoprotein levels, obesity, and smoking were estimated from individual-level patient data pooled from population-based surveys (1998–2007, n = 31,009) from eight LAC countries and from a national survey of the United States (US) population (1999–2004) Age and gender specific prevalence were estimated and age-gender adjusted comparisons between both populations were conducted. Prevalence of diabetes mellitus, hypertension, and low high-density lipoprotein (HDL)-cholesterol in LAC were 5% (95% confidence interval [95% CI]: 3.4, 7.9), 20.2% (95% CI: 12.5, 31), and 53.3% (95% CI: 47, 63.4), respectively. Compared to LAC region’s average, the prevalence of each risk factor tended to be lower in Peru and higher in Chile. LAC women had higher prevalence of obesity and low HDL-cholesterol than men. Obesity, hypercholesterolemia, and hypertriglyceridemia were more prevalent in the US population than in LAC population (31 vs. 16.1%, 16.8 vs. 8.9%, and 36.2 vs. 26.5%, respectively). However, the prevalence of low HDL-cholesterol was higher in LAC than in the US (53.3 vs. 33.7%). Conclusions Major cardiovascular risk factors are highly prevalent in LAC region, in particular low HDL-cholesterol. In addition, marked differences do exist in this prevalence profile between LAC and the US. The observed patterns of obesity-related risk factors and their current and future impact on the burden of cardiovascular diseases remain to be explained. PMID:23349785

Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin

PubMed Central

Schwartz, Michal; Travesa, Anna; Martell, Steven W; Forbes, Douglass J

2015-01-01

Nuclear pore complexes (NPCs) form the gateway to the nucleus, mediating virtually all nucleocytoplasmic trafficking. Assembly of a nuclear pore complex requires the organization of many soluble sub-complexes into a final massive structure embedded in the nuclear envelope. By use of a LacI/LacO reporter system, we were able to assess nucleoporin (Nup) interactions, show that they occur with a high level of specificity, and identify nucleoporins sufficient for initiation of the complex process of NPC assembly in vivo. Eleven nucleoporins from different sub-complexes were fused to LacI-CFP and transfected separately into a human cell line containing a stably integrated LacO DNA array. The LacI-Nup fusion proteins, which bound to the array, were examined for their ability to recruit endogenous nucleoporins to the intranuclear LacO site. Many could recruit nucleoporins of the same sub-complex and a number could also recruit other sub-complexes. Strikingly, Nup133 and Nup107 of the Nup107/160 subcomplex and Nup153 and Nup50 of the nuclear pore basket recruited a near full complement of nucleoporins to the LacO array. Furthermore, Nup133 and Nup153 efficiently targeted the LacO array to the nuclear periphery. Our data support a hierarchical, seeded assembly pathway and identify Nup133 and Nup153 as effective “seeds” for NPC assembly. In addition, we show that this system can be applied to functional studies of individual nucleoporin domains as well as to specific nucleoporin disease mutations. We find that the R391H cardiac arrhythmia/sudden death mutation of Nup155 prevents both its subcomplex assembly and nuclear rim targeting of the LacO array. PMID:25602437

Major cardiovascular risk factors in Latin America: a comparison with the United States. The Latin American Consortium of Studies in Obesity (LASO).

PubMed

Miranda, J Jaime; Herrera, Victor M; Chirinos, Julio A; Gómez, Luis F; Perel, Pablo; Pichardo, Rafael; González, Angel; Sánchez, José R; Ferreccio, Catterina; Aguilera, Ximena; Silva, Eglé; Oróstegui, Myriam; Medina-Lezama, Josefina; Pérez, Cynthia M; Suárez, Erick; Ortiz, Ana P; Rosero, Luis; Schapochnik, Noberto; Ortiz, Zulma; Ferrante, Daniel; Casas, Juan P; Bautista, Leonelo E

2013-01-01

Limited knowledge on the prevalence and distribution of risk factors impairs the planning and implementation of cardiovascular prevention programs in the Latin American and Caribbean (LAC) region. Prevalence of hypertension, diabetes mellitus, abnormal lipoprotein levels, obesity, and smoking were estimated from individual-level patient data pooled from population-based surveys (1998-2007, n=31,009) from eight LAC countries and from a national survey of the United States (US) population (1999-2004) Age and gender specific prevalence were estimated and age-gender adjusted comparisons between both populations were conducted. Prevalence of diabetes mellitus, hypertension, and low high-density lipoprotein (HDL)-cholesterol in LAC were 5% (95% confidence interval [95% CI]: 3.4, 7.9), 20.2% (95% CI: 12.5, 31), and 53.3% (95% CI: 47, 63.4), respectively. Compared to LAC region's average, the prevalence of each risk factor tended to be lower in Peru and higher in Chile. LAC women had higher prevalence of obesity and low HDL-cholesterol than men. Obesity, hypercholesterolemia, and hypertriglyceridemia were more prevalent in the US population than in LAC population (31 vs. 16.1%, 16.8 vs. 8.9%, and 36.2 vs. 26.5%, respectively). However, the prevalence of low HDL-cholesterol was higher in LAC than in the US (53.3 vs. 33.7%). Major cardiovascular risk factors are highly prevalent in LAC region, in particular low HDL-cholesterol. In addition, marked differences do exist in this prevalence profile between LAC and the US. The observed patterns of obesity-related risk factors and their current and future impact on the burden of cardiovascular diseases remain to be explained.

Laser-Assisted Cold-Sprayed Corrosion- and Wear-Resistant Coatings: A Review

NASA Astrophysics Data System (ADS)

Olakanmi, E. O.; Doyoyo, M.

2014-06-01

Laser-assisted cold spray (LACS) process will be increasingly employed for depositing coatings because of its unique advantages: solid-state deposition of dense, homogeneous, and pore-free coatings onto a range of substrates; and high build rate at reduced operating costs without the use of expensive heating and process inert gases. Depositing coatings with excellent performance indicators via LACS demands an accurate knowledge and control of processing and materials' variables. By varying the LACS process parameters and their interactions, the functional properties of coatings can be manipulated. Moreover, thermal effect due to laser irradiation and microstructural evolution complicate the interpretation of LACS mechanical deformation mechanism which is essential for elucidating its physical phenomena. In order to provide a basis for follow-on-research that leads to the development of high-productivity LACS processing of coatings, this review focuses on the latest developments in depositing corrosion- and wear-resistant coatings with the emphasis on the composition, structure, and mechanical and functional properties. Historical developments and fundamentals of LACS are addressed in an attempt to describe the physics behind the process. Typical technological applications of LACS coatings are also identified. The investigations of all process sequences, from laser irradiation of the powder-laden gas stream and the substrate, to the impingement of thermally softened particles on the deposition site, and subsequent further processes, are described. Existing gaps in the literature relating to LACS-dependent microstructural evolution, mechanical deformation mechanisms, correlation between functional properties and process parameters, processing challenges, and industrial applications have been identified in order to provide insights for further investigations and innovation in LACS deposition of wear- and corrosion-resistant coatings.

Light-induced conformational changes of LOV1 (light oxygen voltage-sensing domain 1) and LOV2 relative to the kinase domain and regulation of kinase activity in Chlamydomonas phototropin.

PubMed

Okajima, Koji; Aihara, Yusuke; Takayama, Yuki; Nakajima, Mihoko; Kashojiya, Sachiko; Hikima, Takaaki; Oroguchi, Tomotaka; Kobayashi, Amane; Sekiguchi, Yuki; Yamamoto, Masaki; Suzuki, Tomomi; Nagatani, Akira; Nakasako, Masayoshi; Tokutomi, Satoru

2014-01-03

Phototropin (phot), a blue light (BL) receptor in plants, has two photoreceptive domains named LOV1 and LOV2 as well as a Ser/Thr kinase domain (KD) and acts as a BL-regulated protein kinase. A LOV domain harbors a flavin mononucleotide that undergoes a cyclic photoreaction upon BL excitation via a signaling state in which the inhibition of the kinase activity by LOV2 is negated. To understand the molecular mechanism underlying the BL-dependent activation of the kinase, the photochemistry, kinase activity, and molecular structure were studied with the phot of Chlamydomonas reinhardtii. Full-length and LOV2-KD samples of C. reinhardtii phot showed cyclic photoreaction characteristics with the activation of LOV- and BL-dependent kinase. Truncation of LOV1 decreased the photosensitivity of the kinase activation, which was well explained by the fact that the signaling state lasted for a shorter period of time compared with that of the phot. Small angle x-ray scattering revealed monomeric forms of the proteins in solution and detected BL-dependent conformational changes, suggesting an extension of the global molecular shapes of both samples. Constructed molecular model of full-length phot based on the small angle x-ray scattering data proved the arrangement of LOV1, LOV2, and KD for the first time that showed a tandem arrangement both in the dark and under BL irradiation. The models suggest that LOV1 alters its position relative to LOV2-KD under BL irradiation. This finding demonstrates that LOV1 may interact with LOV2 and modify the photosensitivity of the kinase activation through alteration of the duration of the signaling state in LOV2.

MicroRNA signatures in B-cell lymphomas

PubMed Central

Di Lisio, L; Sánchez-Beato, M; Gómez-López, G; Rodríguez, M E; Montes-Moreno, S; Mollejo, M; Menárguez, J; Martínez, M A; Alves, F J; Pisano, D G; Piris, M A; Martínez, N

2012-01-01

Accurate lymphoma diagnosis, prognosis and therapy still require additional markers. We explore the potential relevance of microRNA (miRNA) expression in a large series that included all major B-cell non-Hodgkin lymphoma (NHL) types. The data generated were also used to identify miRNAs differentially expressed in Burkitt lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL) samples. A series of 147 NHL samples and 15 controls were hybridized on a human miRNA one-color platform containing probes for 470 human miRNAs. Each lymphoma type was compared against the entire set of NHLs. BL was also directly compared with DLBCL, and 43 preselected miRNAs were analyzed in a new series of routinely processed samples of 28 BLs and 43 DLBCLs using quantitative reverse transcription-polymerase chain reaction. A signature of 128 miRNAs enabled the characterization of lymphoma neoplasms, reflecting the lymphoma type, cell of origin and/or discrete oncogene alterations. Comparative analysis of BL and DLBCL yielded 19 differentially expressed miRNAs, which were confirmed in a second confirmation series of 71 paraffin-embedded samples. The set of differentially expressed miRNAs found here expands the range of potential diagnostic markers for lymphoma diagnosis, especially when differential diagnosis of BL and DLBCL is required. PMID:22829247

Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia

PubMed Central

Hiblot, Julien; Bzdrenga, Janek; Champion, Charlotte; Chabriere, Eric; Elias, Mikael

2015-01-01

A new representative of the Phosphotriesterase-Like Lactonases (PLLs) family from the hyperthermophilic crenarchaeon Vulcanisaeta moutnovskia has been characterized and crystallized. VmoLac is a native, proficient lactonase with promiscuous, low phosphotriesterase activity. VmoLac therefore represents an interesting candidate for engineering studies, with the aim of developing an efficient bacterial quorum-quenching agent. Here, we provide an extensive biochemical and kinetic characterization of VmoLac and describe the X-ray structures of the enzyme bound to a fatty acid and to its cognate substrate 3-oxo-C10 AHL (Acyl-Homoserine Lactone). The structures highlight possible structural determinants that may be involved in its extreme thermal stability (Tm = 128°C). Moreover, the structure reveals that the substrate binding mode of VmoLac significantly differs from those of its close homologues, possibly explaining the substrate specificity of the enzyme. Finally, we describe the specific interactions between the enzyme and its substrate, and discuss the possible lactone hydrolysis mechanism of VmoLac. PMID:25670483

Plasticity of laccase generated by homeologous recombination in yeast.

PubMed

Cusano, Angela M; Mekmouche, Yasmina; Meglecz, Emese; Tron, Thierry

2009-10-01

Laccase-encoding sequences sharing 65-71% identity were shuffledin vivo by homeologous recombination. Yeast efficiently repaired linearized plasmids containing clac1, clac2 or clac5 Trametes sp. C30 cDNAs using a clac3 PCR fragment. From transformants secreting active variants, three chimeric laccases (LAC131, LAC232 and LAC535), each resulting from double crossovers, were purified, and their apparent kinetic parameters were determined using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) and syringaldazine (SGZ) as substrates. At acidic pH, the apparent kinetic parameters of the chimera were not distinguishable from each other or from those obtained for the LAC3 enzyme used as reference. On the other hand, the pH tolerance of the variants was visibly extended towards alkaline pH values. Compared to the parental LAC3, a 31-fold increase in apparent k(cat) was observed for LAC131 at pH 8. This factor is one of the highest ever observed for laccase in a single mutagenesis step.

Solving a discrete model of the lac operon using Z3

NASA Astrophysics Data System (ADS)

Gutierrez, Natalia A.

2014-05-01

A discrete model for the Lcac Operon is solved using the SMT-solver Z3. Traditionally the Lac Operon is formulated in a continuous math model. This model is a system of ordinary differential equations. Here, it was considerated as a discrete model, based on a Boolean red. The biological problem of Lac Operon is enunciated as a problem of Boolean satisfiability, and it is solved using an STM-solver named Z3. Z3 is a powerful solver that allows understanding the basic dynamic of the Lac Operon in an easier and more efficient way. The multi-stability of the Lac Operon can be easily computed with Z3. The code that solves the Boolean red can be written in Python language or SMT-Lib language. Both languages were used in local version of the program as online version of Z3. For future investigations it is proposed to solve the Boolean red of Lac Operon using others SMT-solvers as cvc4, alt-ergo, mathsat and yices.

Laccase Down-Regulation Causes Alterations in Phenolic Metabolism and Cell Wall Structure in Poplar1

PubMed Central

Ranocha, Philippe; Chabannes, Matthieu; Chamayou, Simon; Danoun, Saïda; Jauneau, Alain; Boudet, Alain-M.; Goffner, Deborah

2002-01-01

Laccases are encoded by multigene families in plants. Previously, we reported the cloning and characterization of five divergent laccase genes from poplar (Populus trichocarpa) xylem. To investigate the role of individual laccase genes in plant development, and more particularly in lignification, three independent populations of antisense poplar plants, lac3AS, lac90AS, and lac110AS with significantly reduced levels of laccase expression were generated. A repression of laccase gene expression had no effect on overall growth and development. Moreover, neither lignin content nor composition was significantly altered as a result of laccase suppression. However, one of the transgenic populations, lac3AS, exhibited a 2- to 3-fold increase in total soluble phenolic content. As indicated by toluidine blue staining, these phenolics preferentially accumulate in xylem ray parenchyma cells. In addition, light and electron microscopic observations of lac3AS stems indicated that lac3 gene suppression led to a dramatic alteration of xylem fiber cell walls. Individual fiber cells were severely deformed, exhibiting modifications in fluorescence emission at the primary wall/middle lamella region and frequent sites of cell wall detachment. Although a direct correlation between laccase gene expression and lignification could not be assigned, we show that the gene product of lac3 is essential for normal cell wall structure and integrity in xylem fibers. lac3AS plants provide a unique opportunity to explore laccase function in plants. PMID:12011346

Troponin T3 expression in skeletal and smooth muscle is required for growth and postnatal survival: characterization of Tnnt3(tm2a(KOMP)Wtsi) mice.

PubMed

Ju, Yawen; Li, Jie; Xie, Chao; Ritchlin, Christopher T; Xing, Lianping; Hilton, Matthew J; Schwarz, Edward M

2013-09-01

The troponin complex, which consists of three regulatory proteins (troponin C, troponin I, and troponin T), is known to regulate muscle contraction in skeletal and cardiac muscle, but its role in smooth muscle remains controversial. Troponin T3 (TnnT3) is a fast skeletal muscle troponin believed to be expressed only in skeletal muscle cells. To determine the in vivo function and tissue-specific expression of Tnnt3, we obtained the heterozygous Tnnt3+/flox/lacZ mice from Knockout Mouse Project (KOMP) Repository. Tnnt3(lacZ/+) mice are smaller than their WT littermates throughout development but do not display any gross phenotypes. Tnnt3(lacZ/lacZ) embryos are smaller than heterozygotes and die shortly after birth. Histology revealed hemorrhagic tissue in Tnnt3(lacZ/lacZ) liver and kidney, which was not present in Tnnt3(lacZ/+) or WT, but no other gross tissue abnormalities. X-gal staining for Tnnt3 promoter-driven lacZ transgene expression revealed positive staining in skeletal muscle and diaphragm and smooth muscle cells located in the aorta, bladder, and bronchus. Collectively, these findings suggest that troponins are expressed in smooth muscle and are required for normal growth and breathing for postnatal survival. Moreover, future studies with this mouse model can explore TnnT3 function in adult muscle function using the conditional-inducible gene deletion approach Copyright © 2013 Wiley Periodicals, Inc.

Structural dynamics of the lac repressor-DNA complex revealed by a multiscale simulation.

PubMed

Villa, Elizabeth; Balaeff, Alexander; Schulten, Klaus

2005-05-10

A multiscale simulation of a complex between the lac repressor protein (LacI) and a 107-bp-long DNA segment is reported. The complex between the repressor and two operator DNA segments is described by all-atom molecular dynamics; the size of the simulated system comprises either 226,000 or 314,000 atoms. The DNA loop connecting the operators is modeled as a continuous elastic ribbon, described mathematically by the nonlinear Kirchhoff differential equations with boundary conditions obtained from the coordinates of the terminal base pairs of each operator. The forces stemming from the looped DNA are included in the molecular dynamics simulations; the loop structure and the forces are continuously recomputed because the protein motions during the simulations shift the operators and the presumed termini of the loop. The simulations reveal the structural dynamics of the LacI-DNA complex in unprecedented detail. The multiple domains of LacI exhibit remarkable structural stability during the simulation, moving much like rigid bodies. LacI is shown to absorb the strain from the looped DNA mainly through its mobile DNA-binding head groups. Even with large fluctuating forces applied, the head groups tilt strongly and keep their grip on the operator DNA, while the remainder of the protein retains its V-shaped structure. A simulated opening of the cleft of LacI by 500-pN forces revealed the interactions responsible for locking LacI in the V-conformation.

Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers.

PubMed

Cunha, M N M; Felgueiras, H P; Gouveia, I; Zille, A

2017-06-01

Silver nanoparticles (AgNPs) were synthesized by citrate reduction method in the presence of polymers, poly(ethylene glycol) (PEG), poly(vinyl alcohol) (PVA) and chitosan, used as stabilizing agents, and an oxidoreductase enzyme, laccase (Lac), with the goal of expanding the NPs antimicrobial action. AgNPs were characterized by UV-vis spectrometry, dynamic light scattering and transmission electron microscopy. As protecting agents, PEG and PVA promoted the formation of spherical uniformly-shaped, small-sized, monodispersed AgNPs (≈20nm). High Mw polymers were established as most effective in producing small-sized NPs. Chitosan's viscosity led to the formation of aggregates. Despite the decrease in Lac activity registered for the hybrid formulation, AgNPs-polymer-Lac, a significant augment in stability over time (up to 13days, at 50°C) was observed. This novel formulation displays improved synergistic performance over AgNPs-Lac or polymer-Lac conjugates, since in the former the Lac activity becomes residual at the end of 3days. By enabling many ionic interactions, chitosan restricted the mass transfer between Lac and substrate and, thus, inhibited the enzymatic activity. These hybrid nanocomposites made up of inorganic NPs, organic polymers and immobilized antimicrobial oxidoreductive enzymes represent a new class of materials with improved synergistic performance. Moreover, the Lac and the AgNPs different antimicrobial action, both in time and mechanism, may also constitute a new alternative to reduce the probability of developing resistance-associated mutations. Copyright © 2017 Elsevier B.V. All rights reserved.

Effects of Americium-241 and humic substances on Photobacterium phosphoreum: bioluminescence and diffuse reflectance FTIR spectroscopic studies.

PubMed

Kamnev, Alexander A; Tugarova, Anna V; Selivanova, Maria A; Tarantilis, Petros A; Polissiou, Moschos G; Kudryasheva, Nadezhda S

2013-01-01

The integral bioluminescence (BL) intensity of live Photobacterium phosphoreum cells (strain 1883 IBSO), sampled at the stationary growth stage (20 h), was monitored for further 300 h in the absence (control) and presence of (241)Am (an α-emitting radionuclide of a high specific activity) in the growth medium. The activity concentration of (241)Am was 2 kBq l(-1); [(241)Am]=6.5×10(-11) M. Parallel experiments were also performed with water-soluble humic substances (HS, 2.5 mg l(-1); containing over 70% potassium humate) added to the culture medium as a possible detoxifying agent. The BL spectra of all the bacterial samples were very similar (λ(max)=481±3 nm; FWHM=83±3 nm) showing that (241)Am (also with HS) influenced the bacterial BL system at stages prior to the formation of electronically excited states. The HS added per se virtually did not influence the integral BL intensity. In the presence of (241)Am, BL was initially activated but inhibited after 180 h, while the system (241)Am+HS showed an effective activation of BL up to 300 h which slowly decreased with time. Diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, applied to dry cell biomass sampled at the stationary growth phase, was used to control possible metabolic responses of the bacteria to the α-radioactivity stress (observed earlier for other bacteria under other stresses). The DRIFT spectra were all very similar showing a low content of intracellular poly-3-hydroxybutyrate (at the level of a few percent of dry biomass) and no or negligible spectroscopic changes in the presence of (241)Am and/or HS. This assumes the α-radioactivity effect to be transmitted by live cells mainly to the bacterial BL enzyme system, with negligible structural or compositional changes in cellular macrocomponents at the stationary growth phase. Copyright © 2012 Elsevier B.V. All rights reserved.

Effects of americium-241 and humic substances on Photobacterium phosphoreum: Bioluminescence and diffuse reflectance FTIR spectroscopic studies

NASA Astrophysics Data System (ADS)

Kamnev, Alexander A.; Tugarova, Anna V.; Selivanova, Maria A.; Tarantilis, Petros A.; Polissiou, Moschos G.; Kudryasheva, Nadezhda S.

The integral bioluminescence (BL) intensity of live Photobacterium phosphoreum cells (strain 1883 IBSO), sampled at the stationary growth stage (20 h), was monitored for further 300 h in the absence (control) and presence of 241Am (an α-emitting radionuclide of a high specific activity) in the growth medium. The activity concentration of 241Am was 2 kBq l-1; [241Am] = 6.5 × 10-11 M. Parallel experiments were also performed with water-soluble humic substances (HS, 2.5 mg l-1; containing over 70% potassium humate) added to the culture medium as a possible detoxifying agent. The BL spectra of all the bacterial samples were very similar (λmax = 481 ± 3 nm; FWHM = 83 ± 3 nm) showing that 241Am (also with HS) influenced the bacterial BL system at stages prior to the formation of electronically excited states. The HS added per se virtually did not influence the integral BL intensity. In the presence of 241Am, BL was initially activated but inhibited after 180 h, while the system 241Am + HS showed an effective activation of BL up to 300 h which slowly decreased with time. Diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, applied to dry cell biomass sampled at the stationary growth phase, was used to control possible metabolic responses of the bacteria to the α-radioactivity stress (observed earlier for other bacteria under other stresses). The DRIFT spectra were all very similar showing a low content of intracellular poly-3-hydroxybutyrate (at the level of a few percent of dry biomass) and no or negligible spectroscopic changes in the presence of 241Am and/or HS. This assumes the α-radioactivity effect to be transmitted by live cells mainly to the bacterial BL enzyme system, with negligible structural or compositional changes in cellular macrocomponents at the stationary growth phase.

Investigation of drug-excipient compatibility using rheological and thermal tools

NASA Astrophysics Data System (ADS)

Trivedi, Maitri R.

HYPOTHESIS: We plan to investigate a different approach to evaluate drug-excipient physical compatibility using rheological and thermal tools as opposed to commonly used chemical techniques in pharmaceutical industry. This approach offers practical solutions to routinely associated problems arising with API's and commonly used hydrates forms of excipients. ABSTRACT: Drug-Excipient compatibility studies are an important aspect of pre-formulation and formulation development in pharmaceutical research and development. Various approaches have been used in pharmaceutical industry including use of thermal analysis and quantitative assessment of drug-excipient mixtures after keeping the samples under stress environment depending upon the type of formulation. In an attempt to provide better understanding of such compatibility aspect of excipients with different properties of API, various rheological and thermal studies were conducted on binary mixtures of excipients which exist in different hydrates. Dibasic Calcium Phosphate (DCP, anhydrous and dihydrate forms) and Lactose (Lac, anhydrous and monohydrate) were selected with cohesive API's (Acetaminophen and Aspirin). Binary mixtures of DCP and Lac were prepared by addition of 0% w/w to 50% w/w of the API into each powder blend. Rheological and thermal aspects were considered using different approaches such as powder rheometer, rotational shear cell and traditional rheometery approaches like angle of repose (AOR), hausner's ratio (HR) and cares index (CI). Thermal analysis was conducted using modulated differential scanning calorimetry (MDSC) and thermal effusivity. The data suggested that the powder rheometer showed distinctive understanding in the flowability behavior of binary mixtures with addition of increasing proportion of API's than traditional approaches. Thermal approaches revealed the potential interaction of water of crystallization DCP-D with the API (APAP) while such interactions were absent in DCP-A, while in case of Lac-M and Lac-A, interaction with water of crystallization were not present. Binary mixtures prepared with DCP-D were better flowable while blends with DCP-A were better in stability (physical), compressibility and permeability. Similarly binary mixtures prepared with Lac-M were better flowable and stable in physical compatibility as compared to Lac-A. Lac-A were better in compressibility and permeability. Second part of these research included understanding the powder behavior from wet granulation point of view. Wet granulation includes the formation of agglomerates with powders to form granules in order to have better flowability, content uniformity and compressibility of granular mass. End point determination of powders involving change in powder energies and compressibility, permeability along with thermal analyses were conducted. The effects of water of crystallization on end point determination was studied and based on which overall effects on drug-excipient compatibility using different hydrate forms of excipients were evaluated.

Latin America: A Development Pole for Phenomics

PubMed Central

Camargo, Anyela V.; Lobos, Gustavo A.

2016-01-01

Latin America and the Caribbean (LAC) has long been associated with the production and export of a diverse range of agricultural commodities. Due to its strategic geographic location, which encompasses a wide range of climates, it is possible to produce almost any crop. The climate diversity in LAC is a major factor in its agricultural potential but this also means climate change represents a real threat to the region. Therefore, LAC farming must prepare and quickly adapt to an environment that is likely to feature long periods of drought, excessive rainfall and extreme temperatures. With the aim of moving toward a more resilient agriculture, LAC scientists have created the Latin American Plant Phenomics Network (LatPPN) which focuses on LAC's economically important crops. LatPPN's key strategies to achieve its main goal are: (1) training of LAC members on plant phenomics and phenotyping, (2) establish international and multidisciplinary collaborations, (3) develop standards for data exchange and research protocols, (4) share equipment and infrastructure, (5) disseminate data and research results, (6) identify funding opportunities and (7) develop strategies to guarantee LatPPN's relevance and sustainability across time. Despite the challenges ahead, LatPPN represents a big step forward toward the consolidation of a common mind-set in the field of plant phenotyping and phenomics in LAC. PMID:27999577

Latin America: A Development Pole for Phenomics.

PubMed

Camargo, Anyela V; Lobos, Gustavo A

2016-01-01

Latin America and the Caribbean (LAC) has long been associated with the production and export of a diverse range of agricultural commodities. Due to its strategic geographic location, which encompasses a wide range of climates, it is possible to produce almost any crop. The climate diversity in LAC is a major factor in its agricultural potential but this also means climate change represents a real threat to the region. Therefore, LAC farming must prepare and quickly adapt to an environment that is likely to feature long periods of drought, excessive rainfall and extreme temperatures. With the aim of moving toward a more resilient agriculture, LAC scientists have created the Latin American Plant Phenomics Network (LatPPN) which focuses on LAC's economically important crops. LatPPN's key strategies to achieve its main goal are: (1) training of LAC members on plant phenomics and phenotyping, (2) establish international and multidisciplinary collaborations, (3) develop standards for data exchange and research protocols, (4) share equipment and infrastructure, (5) disseminate data and research results, (6) identify funding opportunities and (7) develop strategies to guarantee LatPPN's relevance and sustainability across time. Despite the challenges ahead, LatPPN represents a big step forward toward the consolidation of a common mind-set in the field of plant phenotyping and phenomics in LAC.

Population Dynamics of a Lac(-) Strain of Escherichia Coli during Selection for Lactose Utilization

PubMed Central

Foster, P. L.

1994-01-01

During selection for lactose utilization, Lac(+) revertants of FC40, a Lac(-) strain of Escherichia coli, appear at a high rate. Yet, no Lac(+) revertants appear in the absence of lactose, or in its presence if the cells have another, unfulfilled requirement for growth. This study investigates more fully the population dynamics of FC40 when incubated in the absence of a carbon source or when undergoing selection for lactose utilization. In the absence of a carbon source, the viable cell numbers do not change over 6 days. When incubated in liquid lactose medium, Lac(-) cells do not undergo any measurable increase in numbers or in turbidity for at least 2 days. When FC40 is plated on lactose minimum medium in the presence of scavenger cells, the upper limit to the amount of growth of Lac(-) cells during 5 days is one doubling, and there is no evidence for turnover (i.e., a balance between growth and death). The presence of a minority population that could form microcolonies was not detected. The implications of these results, plus the fact that the appearance of Lac(+) revertants during lactose selection is nearly constant with time, are discussed in reference to several models that have been postulated to account for adaptive mutations. PMID:7828809

Unraveling the nexus between water and food security in Latin America and the Caribbean: regional and global implications

NASA Astrophysics Data System (ADS)

Willaarts, Barbara; Garrido, Alberto; Soriano, Barbara; De Stefano, Lucia; López Gunn, Elena; Aldaya, Maite; Martínez-Santos, Pedro; Llamas, Ramon

2014-05-01

Latin American and the Caribbean (LAC) is a water and land abundant region, and plays a key role in meeting global food and water security. During the last decade, LAC has experience a rapid socio-economic growth, largely sustained by its competitive advantage in the production and exports of agricultural and mining products and by the high commodity prices in the global market. This study seeks to quantify the contribution of LAC's agriculture to global food and water security, i.e. virtual water trade, and evaluate the environmental and societal implications for regional development. Results show that between 2000 and 2011, LAC has increase its agricultural production 27%, and it now accounts for nearly 18% of the global agricultural market. As a result, the agricultural water footprint (WF) of LAC was augmented 65%; and yet, nearly 19% to 44% of the actual agricultural WF - depending on the countries - is virtual water exported to third countries. In fact, almost 50% of the increase in global virtual water trade during the last decade, corresponds to LAC. Such global contribution has significant implications for regional water and food security. From an environmental perspective, crop expansion (mostly rain-fed) resulted in the deforestation of nearly 1 million km2, turning this region into the second most important deforestation hotspots worldwide. This land clearing is having large impacts of ecosystem services, e.g. carbon sequestration, water quality or biodiversity conservation. From a socio-economic perspective, increasing agricultural production has improved regional food security indicators, although one every seven children is still stunted in LAC and nearly 10% of the population remains undernourished. Dietary shifts and socio-cultural factors also lag behind the growing problem of malnutrition in the region, i.e. overweight and obesity. Improvements of water access and sanitation, have had a positive impact on food security indicators, especially among the high-income LAC countries. We conclude that despite the large contribution of LAC's agriculture to global water and food security, this goal is at present intensively tapping into LAC's natural capital. Also, regional improvements in water security have improved, but important goals remain and new challenges are emerging. Water governance in LAC is evolving to address the challenges posed by rapid socio-economic changes, however, as is often the case, the implementation of reforms lags behind.

Maternal Therapy with Ad.VEGF-A165 Increases Fetal Weight at Term in a Guinea-Pig Model of Fetal Growth Restriction.

PubMed

Swanson, Anna M; Rossi, Carlo A; Ofir, Keren; Mehta, Vedanta; Boyd, Michael; Barker, Hannah; Ledwozyw, Agata; Vaughan, Owen; Martin, John; Zachary, Ian; Sebire, Neil; Peebles, Donald M; David, Anna L

2016-12-01

In a model of growth-restricted sheep pregnancy, it was previously demonstrated that transient uterine artery VEGF overexpression can improve fetal growth. This approach was tested in guinea-pig pregnancies, where placental physiology is more similar to humans. Fetal growth restriction (FGR) was attained through peri-conceptual nutrient restriction in virgin guinea pigs. Ad.VEGF-A 165 or Ad.LacZ (1 × 10 10 vp) was applied at mid-gestation via laparotomy, delivered externally to the uterine circulation with thermosensitive gel. At short-term (3-8 days post surgery) or at term gestation, pups were weighed, and tissues were sampled for vector spread analysis, VEGF expression, and its downstream effects. Fetal weight at term was increased (88.01 ± 13.36 g; n = 26) in Ad.VEGF-A 165 -treated animals compared with Ad.LacZ-treated animals (85.52 ± 13.00 g; n = 19; p = 0.028). The brain, liver, and lung weight and crown rump length were significantly larger in short-term analyses, as well as VEGF expression in transduced tissues. At term, molecular analyses confirmed the presence of VEGF transgene in target tissues but not in fetal samples. Tissue histology analysis and blood biochemistry/hematological examination were comparable with controls. Uterine artery relaxation in Ad.VEGF-A 165 -treated dams was higher compared with Ad.LacZ-treated dams. Maternal uterine artery Ad.VEGF-A 165 increases fetal growth velocity and term fetal weight in growth-restricted guinea-pig pregnancy.

Relations between a novel, reliable, and rapid index of arterial compliance (PP-HDI) and well-established inidices of arterial blood pressure (ABP) in a sample of hypertensive elderly subjects.

PubMed

Bergamini, L; Finelli, M E; Bendini, C; Ferrari, E; Veschi, M; Neviani, F; Manni, B; Pelosi, A; Rioli, G; Neri, M

2009-01-01

Hypertension is a risk factor for a long-lasting arterial wall-remodelling leading to stiffness. The rapid method measuring the pulse pressure (PP) by means of the tool of Hypertension Diagnostic Instruments (HDI) called PP-HDI, overcomes some of the problems arising with more-time consuming methods, like ambulatory blood pressure monitoring (ABPM), and give information about the elasticity of the arterial walls. We studied the relationship between the PP-HDI, the large artery compliance (LA-C) and small artery compliance (SA-C) and few well-established indices of arterial blood pressure (ABP) in a sample of 75 hypertensive subjects, aged 65 years and over. Significant correlations between LA-C and heart rate (HR), PP-ABPM and PP-HDI were found. SA-C relates with HR and systolic blood pressure (SBP) measured in lying and standing positions. Applying a stepwise regression analysis, we found that LA-C variance stems from PP-HDI and HR, while SA-C variance stems from SBP in lying position. Receiver operator characteristic (ROC) curves for thresholds of PP showed that PP-HDI reached levels of sensitivity/specificity similar to PP-ABPM. In conclusion, surveillance of ABP through hemo-dynamic indices, in particular of SBP, is essential, nevertheless the advantage of this control is not known in an elderly population where the organ damage is already evident. PP needs necessarily an instrumental measurement. The PP-HDI result is similar in reliability with respect to PPABPM, but is more rapid and well applicable in an elderly population.

Eye drop delivery of nano-polymeric micelle formulated genes with cornea-specific promoters.

PubMed

Tong, Yaw-Chong; Chang, Shwu-Fen; Liu, Chia-Yang; Kao, Winston W-Y; Huang, Chong Heng; Liaw, Jiahorng

2007-11-01

This study evaluates the eye drop delivery of genes with cornea-specific promoters, i.e., keratin 12 (K12) and keratocan (Kera3.2) promoters, by non-ionic poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) polymeric micelles (PM) to mouse and rabbit eyes, and investigates the underlying mechanisms. Three PM-formulated plasmids (pCMV-Lac Z, pK12-Lac Z and pKera3.2-Lac Z) containing the Lac Z gene for beta-galactosidase (beta-Gal) whose expression was driven by the promoter of either the cytomegalovirus early gene, the keratin 12 gene or the keratocan gene, were characterized by critical micelle concentration (CMC), dynamic light scattering (DLS), and atomic force microscopy (AFM). Transgene expression in ocular tissue after gene delivery was analyzed by 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) color staining, 1,2-dioxetane beta-Gal enzymatic activity measurement, and real-time polymerase chain reaction (PCR) analysis. The delivery mechanisms of plasmid-PM on mouse and rabbit corneas were evaluated by EDTA and RGD (arginine-glycine-aspartic acid) peptide. The sizes of the three plasmid-PM complexes were around 150-200 nm with unimodal distribution. Enhanced stability was found for three plasmid-PM formulations after DNase I treatment. After six doses of eye drop delivery of pK12-Lac Z-PM three times a day, beta-Gal activity was significantly increased in both mouse and rabbit corneas. Stroma-specific Lac Z expression was only found in pKera3.2-Lac Z-PM-treated animals with pretreatment by 5 mM EDTA, an opener of junctions. Lac Z gene expression in both pK12-Lac Z-PM and pKera3.2-Lac Z-PM delivery groups was decreased by RGD peptide pretreatment. Cornea epithelium- and stroma-specific gene expression could be achieved using cornea-specific promoters of keratin 12 and keratocan genes, and the gene was delivered with PM formulation through non-invasive, eye drop in mice and rabbits. The transfection mechanism of plasmid-PM may involve endocytosis and particle size dependent paracellular transport. 2007 John Wiley & Sons, Ltd

Polymeric bionanocomposite cast thin films with in situ laccase-catalyzed polymerization of dopamine for biosensing and biofuel cell applications.

PubMed

Tan, Yueming; Deng, Wenfang; Li, Yunyong; Huang, Zhao; Meng, Yue; Xie, Qingji; Ma, Ming; Yao, Shouzhuo

2010-04-22

We report here on the facile preparation of polymer-enzyme-multiwalled carbon nanotubes (MWCNTs) cast films accompanying in situ laccase (Lac)-catalyzed polymerization for electrochemical biosensing and biofuel cell applications. Lac-catalyzed polymerization of dopamine (DA) as a new substrate was examined in detail by UV-vis spectroscopy, cyclic voltammetry, quartz crystal microbalance, and scanning electron microscopy. Casting the aqueous mixture of DA, Lac and MWCNTs on a glassy carbon electrode (GCE) yielded a robust polydopamine (PDA)-Lac-MWCNTs/GCE that can sense hydroquinone with 643 microA mM(-1) cm(-2) sensitivity and 20-nM detection limit (S/N = 3). The DA substrate yielded the best biosensing performance, as compared with aniline, o-phenylenediamine, or o-aminophenol as the substrate for similar Lac-catalyzed polymerization. Casting the aqueous mixture of DA, glucose oxidase (GOx), Lac, and MWCNTs on a Pt electrode yielded a robust PDA-GOx-Lac-MWCNTs/Pt electrode that exhibits glucose-detection sensitivity of 68.6 microA mM(-1) cm(-2). In addition, 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) diammonium salt (ABTS) was also coimmobilized to yield a PDA-Lac-MWCNTs-ABTS/GCE that can effectively catalyze the reduction of O(2), and it was successfully used as the biocathode of a membraneless glucose/O(2) biofuel cell (BFC) in pH 5.0 Britton-Robinson buffer. The proposed biomacromolecule-immobilization platform based on enzyme-catalyzed polymerization may be useful for preparing many other multifunctional polymeric bionanocomposites for wide applications.

Aflatoxin B₁ and M₁ Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators.

PubMed

Loi, Martina; Fanelli, Francesca; Zucca, Paolo; Liuzzi, Vania C; Quintieri, Laura; Cimmarusti, Maria T; Monaci, Linda; Haidukowski, Miriam; Logrieco, Antonio F; Sanjust, Enrico; Mulè, Giuseppina

2016-08-23

Laccases (LCs) are multicopper oxidases that find application as versatile biocatalysts for the green bioremediation of environmental pollutants and xenobiotics. In this study we elucidate the degrading activity of Lac2 pure enzyme form Pleurotus pulmonarius towards aflatoxin B₁ (AFB₁) and M₁ (AFM₁). LC enzyme was purified using three chromatographic steps and identified as Lac2 through zymogram and LC-MS/MS. The degradation assays were performed in vitro at 25 °C for 72 h in buffer solution. AFB₁ degradation by Lac2 direct oxidation was 23%. Toxin degradation was also investigated in the presence of three redox mediators, (2,2'-azino-bis-[3-ethylbenzothiazoline-6-sulfonic acid]) (ABTS) and two naturally-occurring phenols, acetosyringone (AS) and syringaldehyde (SA). The direct effect of the enzyme and the mediated action of Lac2 with redox mediators univocally proved the correlation between Lac2 activity and aflatoxins degradation. The degradation of AFB₁ was enhanced by the addition of all mediators at 10 mM, with AS being the most effective (90% of degradation). AFM₁ was completely degraded by Lac2 with all mediators at 10 mM. The novelty of this study relies on the identification of a pure enzyme as capable of degrading AFB₁ and, for the first time, AFM₁, and on the evidence that the mechanism of an effective degradation occurs via the mediation of natural phenolic compounds. These results opened new perspective for Lac2 application in the food and feed supply chains as a biotransforming agent of AFB₁ and AFM₁.

Indian Americans at Mille Lacs.

ERIC Educational Resources Information Center

Holbert, Victoria L.; And Others

The Training Center for Community Programs prepared a report on the Mille Lacs (Chippewa) Reservation in Minnesota. Data for the report were from 2 separate sources: a survey conducted by the Training Center with the assistance of the Mille Lacs community action program (1967) and an attitudinal survey conducted by Victoria Holbert during 1969.…

Program Evaluation of Community College Learning Assistance Centers: What Do LAC Directors Think?

ERIC Educational Resources Information Center

Franklin, Doug; Blankenberger, Bob

2016-01-01

Objective: This study seeks to determine the nature of current program evaluation practices for learning assistance centers (LACs), the practices being used for program evaluation, and whether LAC directors believe their practices are appropriate for evaluating program effectiveness. Method: We conducted a survey (n = 61) of community college LAC…

Lactose-induced cell death of beta-galactosidase mutants in Kluyveromyces lactis.

PubMed

Lodi, Tiziana; Donnini, Claudia

2005-05-01

The Kluyveromyces lactis lac4 mutants, lacking the beta-galactosidase gene, cannot assimilate lactose, but grow normally on many other carbon sources. However, when these carbon sources and lactose were simultaneously present in the growth media, the mutants were unable to grow. The effect of lactose was cytotoxic since the addition of lactose to an exponentially-growing culture resulted in 90% loss of viability of the lac4 cells. An osmotic stabilizing agent prevented cells killing, supporting the hypothesis that the lactose toxicity could be mainly due to intracellular osmotic pressure. Deletion of the lactose permease gene, LAC12, abolished the inhibitory effect of lactose and allowed the cell to assimilate other carbon substrates. The lac4 strains gave rise, with unusually high frequency, to spontaneous mutants tolerant to lactose (lar1 mutation: lactose resistant). These mutants were unable to take up lactose. Indeed, lar1 mutation turned out to be allelic to LAC12. The high mutability of the LAC12 locus may be an advantage for survival of K. lactis whose main habitat is lactose-containing niches.

Spectroscopy and formation of lanthanum-hydrocarbon radicals formed by association and carbon-carbon bond cleavage of isoprene

NASA Astrophysics Data System (ADS)

Cao, Wenjin; Hewage, Dilrukshi; Yang, Dong-Sheng

2018-05-01

La atom reaction with isoprene is carried out in a laser-vaporization molecular beam source. The reaction yields an adduct as the major product and C—C cleaved and dehydrogenated species as the minor ones. La(C5H8), La(C2H2), and La(C3H4) are characterized with mass-analyzed threshold ionization (MATI) spectroscopy and quantum chemical computations. The MATI spectra of all three species exhibit a strong origin band and several weak vibronic bands corresponding to La-ligand stretch and ligand-based bend excitations. La(C5H8) is a five-membered metallacycle, whereas La(C2H2) and La(C3H4) are three-membered rings. All three metallacycles prefer a doublet ground state with a La 6s1-based valence electron configuration and a singlet ion. The five-membered metallacycle is formed through La addition and isoprene isomerization, whereas the two three-membered rings are produced by La addition and insertion, hydrogen migration, and carbon-carbon bond cleavage.

VizieR Online Data Catalog: The UV-bright Quasar Survey (UVQS) DR1 (Monroe+, 2016)

NASA Astrophysics Data System (ADS)

Monroe, T. R.; Prochaska, J. X.; Tejos, N.; Worseck, G.; Hennawi, J. F.; Schmidt, T.; Tumlinson, J.; Shen, Y.

2016-09-01

We have performed an all-sky survey for z~1, FUV-bright quasars selected from GALEX and WISE photometry. We generated a list of 1450 primary candidates (Table1). In several of the observing runs, conditions were unexpectedly favorable and we exhausted the primary candidates at certain right ascension ranges. To fill the remaining observing time, we generated a secondary candidate list. This secondary set of candidates is provided in Table2. We proceeded to obtain discovery-quality longslit spectra (i.e., low-dispersion, large wavelength coverage, modest signal-to-noise ratio (S/N) of our UV-bright Quasar Survey (UVQS) candidates in one calendar year. Our principal facilities were: (i) the dual Kast spectrometer on the 3m Shane telescope at the Lick Observatory; (ii) the Boller & Chivens (BCS) spectrometer on the Irenee du Pont 100'' telescope at the Las Campanas Observatory; and (iii) the Calar Alto Faint Object Spectrograph on the CAHA 2.2m telescope at the Calar Alto Observatory (CAHA). We acquired an additional ~20 spectra on larger aperture telescopes (Keck/ESI, MMT/MBC, Magellan/MagE) during twilight or under poor observing conditions. Typical exposure times were limited to <~200s, with adjustments for fainter sources or sub-optimal observing conditions. Table3 provides a list of the observed candidates. There are 93 sources with a good quality spectrum for which we cannot recover a secure redshift. The majority of these have been previously cataloged as blazars (or BL Lac objects). Table6 lists the sample of these unknowns. (6 data files).

Fermi Large Area Telescope Observations Of Misaligned Active Galactic Nuclei

DOE PAGES

Abdo, A. A.

2010-08-13

Analysis is presented for 15 months of data taken with the Large Area Telescope (LAT) on the Fermi Gamma-ray Space Telescope for 11 non-blazar active galactic nuclei (AGNs), including seven FRI radio galaxies and four FRII radio sources consisting of two FRII radio galaxies and two steep spectrum radio quasars. The broad line FRI radio galaxy 3C 120 is reported here as a γ-ray source for the first time. The analysis is based on directional associations of LAT sources with radio sources in the 3CR, 3CRR, and MS4 (collectively referred to as 3C-MS) catalogs. Seven of the eleven LAT sourcesmore » associated with 3C-MS radio sources have spectral indices larger than 2.3 and, except for the FRI radio galaxy NGC 1275 that shows possible spectral curvature, are well described by a power law. No evidence for time variability is found for any sources other than NGC 1275. The γ-ray luminosities of FRI radio galaxies are significantly smaller than those of the BL Lac objects detected by the LAT, whereas the γ-ray luminosities of the FRII sources are quite similar to those of FSRQs, which could reflect different beaming factors for the γ-ray emission. A core dominance (CD) study of the 3CRR sample indicates that sources closer to the jet axis are preferentially detected with the Fermi LAT, insofar as the γ-ray-detected misaligned AGNs have larger CD at a given average radio flux. The results are discussed in view of the AGN unification scenario.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goyal, Arti; Stawarz, Łukasz; Ostrowski, Michał

We present the results of our power spectral analysis for the BL Lac object PKS 0735+178, utilizing the Fermi -LAT survey at high-energy γ -rays, several ground-based optical telescopes, and single-dish radio telescopes operating at GHz frequencies. The novelty of our approach is that, by combining long-term and densely sampled intra-night light curves in the optical regime, we were able to construct for the first time the optical power spectrum of the blazar for a time domain extending from 23 years down to minutes. Our analysis reveals that: (1) the optical variability is consistent with a pure red noise, formore » which the power spectral density can be well approximated by a single power law throughout the entire time domain probed; (2) the slope of power spectral density at high-energy γ -rays (∼1) is significantly flatter than that found at radio and optical frequencies (∼2) within the corresponding time variability range; (3) for the derived power spectra, we did not detect any low-frequency flattening, nor do we see any evidence for cutoffs at the highest frequencies down to the noise floor levels due to measurement uncertainties. We interpret our findings in terms of a model where the blazar variability is generated by the underlying single stochastic process (at radio and optical frequencies), or a linear superposition of such processes (in the γ -ray regime). Along with the detailed PSD analysis, we also present the results of our extended (1998–2015) intra-night optical monitoring program and newly acquired optical photo-polarimetric data for the source.« less

Fermi Large Area Telescope Observations of Misaligned Active Galactic Nuclei

NASA Astrophysics Data System (ADS)

Abdo, A. A.; Ackermann, M.; Ajello, M.; Baldini, L.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Bechtol, K.; Bellazzini, R.; Berenji, B.; Blandford, R. D.; Bloom, E. D.; Bonamente, E.; Borgland, A. W.; Bouvier, A.; Brandt, T. J.; Bregeon, J.; Brez, A.; Brigida, M.; Bruel, P.; Buehler, R.; Burnett, T. H.; Buson, S.; Caliandro, G. A.; Cameron, R. A.; Cannon, A.; Caraveo, P. A.; Carrigan, S.; Casandjian, J. M.; Cavazzuti, E.; Cecchi, C.; Çelik, Ö.; Celotti, A.; Charles, E.; Chekhtman, A.; Chen, A. W.; Cheung, C. C.; Chiang, J.; Ciprini, S.; Claus, R.; Cohen-Tanugi, J.; Colafrancesco, S.; Conrad, J.; Davis, D. S.; Dermer, C. D.; de Angelis, A.; de Palma, F.; Silva, E. do Couto e.; Drell, P. S.; Dubois, R.; Favuzzi, C.; Fegan, S. J.; Ferrara, E. C.; Fortin, P.; Frailis, M.; Fukazawa, Y.; Fusco, P.; Gargano, F.; Gasparrini, D.; Gehrels, N.; Germani, S.; Giglietto, N.; Giommi, P.; Giordano, F.; Giroletti, M.; Glanzman, T.; Godfrey, G.; Grandi, P.; Grenier, I. A.; Grove, J. E.; Guillemot, L.; Guiriec, S.; Hadasch, D.; Hayashida, M.; Hays, E.; Horan, D.; Hughes, R. E.; Jackson, M. S.; Jóhannesson, G.; Johnson, A. S.; Johnson, W. N.; Kamae, T.; Katagiri, H.; Kataoka, J.; Knödlseder, J.; Kuss, M.; Lande, J.; Latronico, L.; Lee, S.-H.; Lemoine-Goumard, M.; Llena Garde, M.; Longo, F.; Loparco, F.; Lott, B.; Lovellette, M. N.; Lubrano, P.; Madejski, G. M.; Makeev, A.; Malaguti, G.; Mazziotta, M. N.; McConville, W.; McEnery, J. E.; Michelson, P. F.; Migliori, G.; Mitthumsiri, W.; Mizuno, T.; Monte, C.; Monzani, M. E.; Morselli, A.; Moskalenko, I. V.; Murgia, S.; Naumann-Godo, M.; Nestoras, I.; Nolan, P. L.; Norris, J. P.; Nuss, E.; Ohsugi, T.; Okumura, A.; Omodei, N.; Orlando, E.; Ormes, J. F.; Paneque, D.; Panetta, J. H.; Parent, D.; Pelassa, V.; Pepe, M.; Persic, M.; Pesce-Rollins, M.; Piron, F.; Porter, T. A.; Rainò, S.; Rando, R.; Razzano, M.; Razzaque, S.; Reimer, A.; Reimer, O.; Reyes, L. C.; Roth, M.; Sadrozinski, H. F.-W.; Sanchez, D.; Sander, A.; Scargle, J. D.; Sgrò, C.; Siskind, E. J.; Smith, P. D.; Spandre, G.; Spinelli, P.; Stawarz, Ł.; Stecker, F. W.; Strickman, M. S.; Suson, D. J.; Takahashi, H.; Tanaka, T.; Thayer, J. B.; Thayer, J. G.; Thompson, D. J.; Tibaldo, L.; Torres, D. F.; Torresi, E.; Tosti, G.; Tramacere, A.; Uchiyama, Y.; Usher, T. L.; Vandenbroucke, J.; Vasileiou, V.; Vilchez, N.; Villata, M.; Vitale, V.; Waite, A. P.; Wang, P.; Winer, B. L.; Wood, K. S.; Yang, Z.; Ylinen, T.; Ziegler, M.

2010-09-01

Analysis is presented for 15 months of data taken with the Large Area Telescope (LAT) on the Fermi Gamma-ray Space Telescope for 11 non-blazar active galactic nuclei (AGNs), including seven FRI radio galaxies and four FRII radio sources consisting of two FRII radio galaxies and two steep spectrum radio quasars. The broad line FRI radio galaxy 3C 120 is reported here as a γ-ray source for the first time. The analysis is based on directional associations of LAT sources with radio sources in the 3CR, 3CRR, and MS4 (collectively referred to as 3C-MS) catalogs. Seven of the eleven LAT sources associated with 3C-MS radio sources have spectral indices larger than 2.3 and, except for the FRI radio galaxy NGC 1275 that shows possible spectral curvature, are well described by a power law. No evidence for time variability is found for any sources other than NGC 1275. The γ-ray luminosities of FRI radio galaxies are significantly smaller than those of the BL Lac objects detected by the LAT, whereas the γ-ray luminosities of the FRII sources are quite similar to those of FSRQs, which could reflect different beaming factors for the γ-ray emission. A core dominance (CD) study of the 3CRR sample indicates that sources closer to the jet axis are preferentially detected with the Fermi LAT, insofar as the γ-ray-detected misaligned AGNs have larger CD at a given average radio flux. The results are discussed in view of the AGN unification scenario.

OPTICAL SPECTRA OF CANDIDATE INTERNATIONAL CELESTIAL REFERENCE FRAME (ICRF) FLAT-SPECTRUM RADIO SOURCES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Titov, O.; Stanford, Laura M.; Johnston, Helen M.

2013-07-01

Continuing our program of spectroscopic observations of International Celestial Reference Frame (ICRF) sources, we present redshifts for 120 quasars and radio galaxies. Data were obtained with five telescopes: the 3.58 m European Southern Observatory New Technology Telescope, the two 8.2 m Gemini telescopes, the 2.5 m Nordic Optical Telescope (NOT), and the 6.0 m Big Azimuthal Telescope of the Special Astrophysical Observatory in Russia. The targets were selected from the International VLBI Service for Geodesy and Astrometry candidate International Celestial Reference Catalog which forms part of an observational very long baseline interferometry (VLBI) program to strengthen the celestial reference frame.more » We obtained spectra of the potential optical counterparts of more than 150 compact flat-spectrum radio sources, and measured redshifts of 120 emission-line objects, together with 19 BL Lac objects. These identifications add significantly to the precise radio-optical frame tie to be undertaken by Gaia, due to be launched in 2013, and to the existing data available for analyzing source proper motions over the celestial sphere. We show that the distribution of redshifts for ICRF sources is consistent with the much larger sample drawn from Faint Images of the Radio Sky at Twenty cm (FIRST) and Sloan Digital Sky Survey, implying that the ultra-compact VLBI sources are not distinguished from the overall radio-loud quasar population. In addition, we obtained NOT spectra for five radio sources from the FIRST and NRAO VLA Sky Survey catalogs, selected on the basis of their red colors, which yielded three quasars with z > 4.« less

A Search for Hyperluminous X-Ray Sources in the XMM-Newton Source Catalog

NASA Astrophysics Data System (ADS)

Zolotukhin, I.; Webb, N. A.; Godet, O.; Bachetti, M.; Barret, D.

2016-02-01

We present a new method to identify luminous off-nuclear X-ray sources in the outskirts of galaxies from large public redshift surveys, distinguishing them from foreground and background interlopers. Using the 3XMM-DR5 catalog of X-ray sources and the SDSS DR12 spectroscopic sample of galaxies, with the help of this off-nuclear cross-matching technique, we selected 98 sources with inferred X-ray luminosities in the range 1041 < LX < 1044 erg s-1, compatible with hyperluminous X-ray objects (HLX). To validate the method, we verify that it allowed us to recover known HLX candidates such as ESO 243-49 HLX-1 and M82 X-1. From a statistical study, we conservatively estimate that up to 71 ± 11 of these sources may be foreground- or background sources, statistically leaving at least 16 that are likely to be HLXs, thus providing support for the existence of the HLX population. We identify two good HLX candidates and using other publicly available data sets, in particular the VLA FIRST in radio, UKIRT Infrared Deep Sky Survey in the near-infrared, GALEX in the ultraviolet and Canada-France-Hawaii Telescope Megacam archive in the optical, we present evidence that these objects are unlikely to be foreground or background X-ray objects of conventional types, e.g., active galactic nuclei, BL Lac objects, Galactic X-ray binaries, or nearby stars. However, additional dedicated X-ray and optical observations are needed to confirm their association with the assumed host galaxies and thus secure their HLX classification.

An in vitro bioassay for xenobiotics using the SXR-driven human CYP3A4/lacZ reporter gene.

PubMed

Lee, Mi R; Kim, Yeon J; Hwang, Dae Y; Kang, Tae S; Hwang, Jin H; Lim, Chae H; Kang, Hyung K; Goo, Jun S; Lim, Hwa J; Ahn, Kwang S; Cho, Jung S; Chae, Kap R; Kim, Yong K

2003-01-01

The dose and time effect of nine xenobiotics, including 17beta-estradiol, corticosterone, dexamethasone, progesterone, nifedipine, bisphenol A, rifampicin, methamphetamine, and nicotine were investigated, in vitro, using human steroid and xenobiotics receptor (SXR)-binding sites on the human CYP3A4 promoter, which can enhance the linked lacZ reporter gene transcription. To test this, liver-specific SAP (human serum amyloid P component)-SXR (SAP/SXR) and human CYP3A4 promoter-regulated lacZ (hCYP3A4/lacZ) constructs were transiently transfected into HepG2 and NIH3T3 cells to compare the xenobiotic responsiveness between human and nonhuman cell lines. In the HepG2 cells, rifampicin, followed by corticosterone, nicotine, methamphetamine, and dexamethasone, exhibited enhanced levels of the lacZ transcript, whereas those of bisphenol A and nifedipine were found to be reduced. No significant responses were observed with 17beta-estradiol or progesterone. In addition, 17beta-estradiol and progesterone did not change the levels of the lacZ transcripts in the HepG2 cells, but did induce significant increases in the transcripts of the NIH3T3 cells. Treatment with corticosterone and dexamethasone, which were highly expressed in the HepG2 cells, did not affect the levels of the lacZ transcript in NIH3T3 cells. These results show that lacZ transcripts can be measured, rapidly and reproducibly, using reverse transcriptase-polymerase chain reaction (RT-PCR) based on the expression of the hCYP3A4/lacZ reporter gene, and was mediated by the SXR. Thus, this in vitro reporter gene bioassay is useful for measuring xenobiotic activities, and is a means to a better relevant bioassay, using human cells, human genes and human promoters, in order to get a closer look at actual human exposure.

Characterization of pLAC1, a cryptic plasmid isolated from Lactobacillus acidipiscis and comparative analysis with its related plasmids.

PubMed

Asteri, Ioanna-Areti; Papadimitriou, Konstantinos; Boutou, Effrossyni; Anastasiou, Rania; Pot, Bruno; Vorgias, Constantinos E; Tsakalidou, Effie

2010-07-15

The pLAC1 plasmid of Lactobacillus acidipiscis ACA-DC 1533, a strain isolated from traditional Kopanisti cheese, was characterised. Nucleotide sequence analysis revealed a circular molecule of 3478bp with a G+C content of 37.2%. Ab initio annotation indicated four putative open reading frames (orfs). orf1 and orf4 were found to encode a replication initiation protein (Rep) and a mobilization protein (Mob), respectively. The deduced products of orf2 and orf3 revealed no significant homology to other known proteins. However, in silico examination of the plasmid sequence supported the existence of a novel operon that includes rep, orf2 and orf3 in pLAC1 and that this operon is highly conserved also in plasmids pLB925A02, pSMA23, pLC88 and pC7. RT-PCR experiments allowed us to verify that these three genes are co-transcribed as a single polycistronic mRNA species. Furthermore, phylogenetic analysis of pLAC1 Rep and Mob proteins demonstrated that they may have derived from different plasmid origins, suggesting that pLAC1 is a product of a modular evolution process. Comparative analysis of full length nucleotide sequences of pLAC1 and related Lactobacillus plasmids showed that pLAC1 shares a very similar replication backbone with pLB925A02, pSMA23 and pLC88. In contrast, mob of pLAC1 was almost identical with the respective gene of plasmids pLAB1000, pLB4 and pPB1. These findings lead to the conclusion that pLAC1 acquired mob probably via an ancestral recombination event. Our overall work highlights the importance of characterizing plasmids deriving from non-starter 'wild' isolates in order to better appreciate plasmid divergence and evolution of lactic acid bacteria. 2010 Elsevier B.V. All rights reserved.

Magnesium for treatment of acute lacunar stroke syndromes: further analysis of the IMAGES trial.

PubMed

Aslanyan, Stella; Weir, Christopher J; Muir, Keith W; Lees, Kennedy R

2007-04-01

A prespecified interaction analysis of the neutral Intravenous Magnesium Efficacy in Stroke (IMAGES) trial revealed significant benefit from magnesium (Mg) in patients with noncortical stroke. Post hoc analysis indicated that this effect was seen in lacunar clinical syndromes (LACS), interaction P=0.005. We have now examined whether this interaction could be explained by confounding baseline factors. LACS was defined on the basis of neurological signs and did not include imaging. We investigated the interaction between baseline variables and Mg treatment on global outcome. We used logistic-regression models to test whether the Mg-LACS interaction remained significant after adjusting for stratification variables, sex, a novel stroke severity score, and baseline variables that had an interaction with treatment (P<0.1). The Mg (n=383) and placebo (n=382) groups of LACS patients were well matched on baseline factors. In addition to LACS, we found an interaction between beneficial Mg treatment effect and younger age (P=0.003), higher baseline diastolic blood pressure (P=0.02), higher mean blood pressure (P=0.02), and absence of ischemic heart disease (P=0.07). Even so, the adjusted Mg-LACS interaction remained significant (odds ratio [OR] 0.57; 95% CI, 0.39 to 0.83; P=0.003). In the LACS subgroup, Mg improved Barthel Index <95 (OR 0.73; 95% CI, 0.55 to 0.98), modified Rankin Scale >1 (OR 0.67; 95% CI, 0.50 to 0.91), and global outcome (OR 0.70; 95% CI, 0.53 to 0.92) but not Barthel Index <60 or mortality. The positive treatment effect of Mg in LACS cannot be ascribed to general issues of severity, time to treatment, blood pressure, or other baseline factors; equally, this finding may be due to chance. A large trial of Mg treatment in LACS appears justified.

α-Lactalbumin and casein-glycomacropeptide do not affect iron absorption from formula in healthy term infants.

PubMed

Szymlek-Gay, Ewa A; Lönnerdal, Bo; Abrams, Steven A; Kvistgaard, Anne S; Domellöf, Magnus; Hernell, Olle

2012-07-01

Iron absorption from infant formula is relatively low. α-Lactalbumin and casein-glycomacropeptide have been suggested to enhance mineral absorption. We therefore assessed the effect of α-lactalbumin and casein-glycomacropeptide on iron absorption from infant formula in healthy term infants. Thirty-one infants were randomly assigned to receive 1 of 3 formulas (4 mg iron/L, 13.1 g protein/L) from 4-8 wk to 6 mo of age: commercially available whey-predominant standard infant formula (standard formula), α-lactalbumin-enriched infant formula (α-LAC), or α-lactalbumin-enriched/casein-glycomacropeptide-reduced infant formula (α-LAC/RGMP). Nine breast-fed infants served as a reference. At 5.5 mo of age, (58)Fe was administered to all infants in a meal. Blood samples were collected 14 d later for iron absorption and iron status indices. Iron deficiency was defined as depleted iron stores, iron-deficient erythropoiesis, or iron deficiency anemia. Iron absorption (mean ± SD) was 10.3 ± 7.0% from standard formula, 8.6 ± 3.8% from α-LAC, 9.2 ± 6.5% from α-LAC/RGMP, and 12.9 ± 6.5% from breast milk, with no difference between the formula groups (P = 0.79) or all groups (P = 0.44). In the formula-fed infants only, iron absorption was negatively correlated with serum ferritin (r = -0.49; P = 0.005) and was higher (P = 0.023) in iron-deficient infants (16.4 ± 12.4%) compared with those with adequate iron status (8.6 ± 4.4%). Our findings indicate that α-lactalbumin and casein-glycomacropeptide do not affect iron absorption from infant formula in infants. Low serum ferritin concentrations are correlated with increased iron absorption from infant formula.

A Search for Quasi-periodic Oscillations in the Blazar 1ES 1959+650

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Xiao-Pan; Luo, Yu-Hui; Yang, Hai-Yan

We have searched quasi-periodic oscillations (QPOs) in the 15 GHz light curve of the BL Lac object 1ES 1959+650 monitored by the Owens Valley Radio Observatory 40 m telescope during the period from 2008 January to 2016 February, using the Lomb–Scargle Periodogram, power spectral density (PSD), discrete autocorrelation function, and phase dispersion minimization (PDM) techniques. The red noise background has been established via the PSD method, and no QPO can be derived at the 3 σ confidence level accounting for the impact of the red noise variability. We conclude that the light curve of 1ES 1959+650 can be explained bymore » a stochastic red noise process that contributes greatly to the total observed variability amplitude, dominates the power spectrum, causes spurious bumps and wiggles in the autocorrelation function and can result in the variance of the folded light curve decreasing toward lower temporal frequencies when few-cycle, sinusoid-like patterns are present. Moreover, many early supposed periodicity claims for blazar light curves need to be reevaluated assuming red noise.« less

Jet outflow and gamma-ray emission correlations in S5 0716+714

DOE PAGES

Rani, B.; Krichbaum, T. P.; Marscher, A. P.; ...

2014-11-06

Here, using millimeter very long baseline interferometry (VLBI) observations of the BL Lac object S5 0716+714 from August 2008 to September 2013, we investigate variations in the core flux density and orientation of the sub-parsec scale jet, i.e. position angle. The γ-ray data obtained by the Fermi Large Area Telescope are used to investigate the high-energy flux variations over the same time period. For the first time in any blazar, we report a significant correlation between the γ-ray flux variations and the position angle variations in the VLBI jet. The cross-correlation analysis also indicates a positive correlation such that themore » mm-VLBI core flux density variations are delayed with respect to the γ-ray flux by 82±32 days. This suggests that the high-energy emission is coming from a region located ≥(3.8±1.9) parsecs upstream of the mm-VLBI core (closer to the central black hole). Lastly, these results imply that the observed inner jet morphology has a strong connection with the observed γ-ray flares.« less

PKS 2155-304 in July 2006: H.E.S.S. results and simultaneous multi-wavelength observations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lenain, Jean-Philippe; Boisson, Catherine; Sol, Helne

2008-12-24

The high-frequency-peaked BL Lac PKS 2155-304 is one of the brightest and best-studied VHE {gamma}-ray sources in the southern hemisphere. The High Energy Stereoscopic System (H.E.S.S.) has monitored PKS 2155-304 in 2006 and a multi-wavelength campaign involving X-ray, optical and radio observatories was triggered by the detection of an active state in July 2006, followed by the detection of two extraordinary flares on July, 28th and 30th, with peak fluxes {approx}100 times the usual values. We present results from the spectral and flux variability analysis of the VHE and simultaneous X-ray observations with Chandra during the second flare, as wellmore » as the detailed evolution of the VHE flux of PKS 2155-304 observed by H.E.S.S. in 2006. A study of flux correlations in the different frequency ranges during the second flare and the adjacent nights is discussed. We also present an interpretation of the active state of PKS 2155-304 in the framework of synchrotron self-Compton emission.« less

Multiband optical-IR variability of the blazar PKS 0537-441

NASA Astrophysics Data System (ADS)

Li, Xiao-Pan; Wang, Li-Sha; Yang, Cheng; Yang, Hai-yan; Zhou, Li; Xu, Guang-Yang; Shan, Yu-Qiong; Liu, Jie; Luo, Yu-Hui; Zhang, Li

2018-06-01

We have reconsidered the simultaneous and homogeneous optical-IR light curves and the corresponding spectral indices curve of the blazar PKS 0537-441 from January 2011 to May 2015. All the curves show significant fluctuations on various timescales, and the flux variations seem to be more pronounced towards the IR bands. The relation between average fluxes and spectral indices reveals the existence of redder-when-brighter (RWB) and bluer-when-brighter (BWB) trends at different flux levels, along with a long-term achromatic trend and a mild RWB trend on short-term timescales. Cross-correlation analyses present an energy-dependent time delay that the lower-frequency variations follow higher-frequency ones by a few weeks and a hysteresis pattern between spectra and fluxes. Our analysis reveals some potential coherence between low-energy-peaked BL Lacs (LBLs) and FSRQs, and indicates that the observed flux variability and spectral changes could be due to the superposition of a dominant jet emission, an underlying thermal contribution from a more slowly varying disk and/or other geometric effects under the shock-in-jet scenario.

Jet outflow and gamma-ray emission correlations in S5 0716+714

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rani, B.; Krichbaum, T. P.; Marscher, A. P.

Here, using millimeter very long baseline interferometry (VLBI) observations of the BL Lac object S5 0716+714 from August 2008 to September 2013, we investigate variations in the core flux density and orientation of the sub-parsec scale jet, i.e. position angle. The γ-ray data obtained by the Fermi Large Area Telescope are used to investigate the high-energy flux variations over the same time period. For the first time in any blazar, we report a significant correlation between the γ-ray flux variations and the position angle variations in the VLBI jet. The cross-correlation analysis also indicates a positive correlation such that themore » mm-VLBI core flux density variations are delayed with respect to the γ-ray flux by 82±32 days. This suggests that the high-energy emission is coming from a region located ≥(3.8±1.9) parsecs upstream of the mm-VLBI core (closer to the central black hole). Lastly, these results imply that the observed inner jet morphology has a strong connection with the observed γ-ray flares.« less

Variable gamma-ray sky at 1 GeV

NASA Astrophysics Data System (ADS)

Pshirkov, M. S.; Rubtsov, G. I.

2013-01-01

We search for the long-term variability of the gamma-ray sky in the energy range E > 1 GeV with 168 weeks of the gamma-ray telescope Fermi-LAT data. We perform a full sky blind search for regions with variable flux looking for deviations from uniformity. We bin the sky into 12288 pixels using the HEALPix package and use the Kolmogorov-Smirnov test to compare weekly photon counts in each pixel with the constant flux hypothesis. The weekly exposure of Fermi-LAT for each pixel is calculated with the Fermi-LAT tools. We consider flux variations in a pixel significant if the statistical probability of uniformity is less than 4 × 10-6, which corresponds to 0.05 false detections in the whole set. We identified 117 variable sources, 27 of which have not been reported variable before. The sources with previously unidentified variability contain 25 active galactic nuclei (AGN) belonging to the blazar class (11 BL Lacs and 14 FSRQs), one AGN of an uncertain type, and one pulsar PSR J0633+1746 (Geminga).

X-ray spectra and time variability of active galactic nuclei

NASA Technical Reports Server (NTRS)

Mushotzky, R. F.

1984-01-01

The X-ray spectra of broad line active galactic nuclei (AGN) of all types (Seyfert I's, NELG's, broadline radio galaxies) are well fit by a power law in the .5 to 100 keV band of man energy slope alpha = .68 + or - .15. There is, as yet, no strong evidence for time variability of this slope in a given object. The constraints that this places on simple models of the central energy source are discussed. BL Lac objects have quite different X-ray spectral properties and show pronounced X-ray spectral variability. On time scales longer than 12 hours most radio quiet AGN do not show strong, delta I/I .5, variability. The probability of variability of these AGN seems to be inversely related to their luminosity. However characteristics timescales for variability have not been measured for many objects. This general lack of variability may imply that most AGN are well below the Eddington limit. Radio bright AGN tend to be more variable than radio quiet AGN on long, tau approx 6 month, timescales.

The effects of adsorbing organic pollutants from super heavy oil wastewater by lignite activated coke.

PubMed

Tong, Kun; Lin, Aiguo; Ji, Guodong; Wang, Dong; Wang, Xinghui

2016-05-05

The adsorption of organic pollutants from super heavy oil wastewater (SHOW) by lignite activated coke (LAC) was investigated. Specifically, the effects of LAC adsorption on pH, BOD5/COD(Cr)(B/C), and the main pollutants before and after adsorption were examined. The removed organic pollutants were characterized by Fourier transform infrared spectroscopy (FTIR), Boehm titrations, gas chromatography-mass spectrometry (GC-MS), and liquid chromatography with organic carbon detection (LC-OCD). FTIR spectra indicated that organic pollutants containing -COOH and -NH2 functional groups were adsorbed from the SHOW. Boehm titrations further demonstrated that carboxyl, phenolic hydroxyl, and lactonic groups on the surface of the LAC increased. GC-MS showed that the removed main organic compounds are difficult to be degraded or extremely toxics to aquatic organisms. According to the results of LC-OCD, 30.37 mg/L of dissolved organic carbons were removed by LAC adsorption. Among these, hydrophobic organic contaminants accounted for 25.03 mg/L. Furthermore, LAC adsorption was found to increase pH and B/C ratio of the SHOW. The mechanisms of adsorption were found to involve between the hydrogen bonding and the functional groups of carboxylic, phenolic, and lactonic on the LAC surface. In summary, all these results demonstrated that LAC adsorption can remove bio-refractory DOCs, which is beneficial for biodegradation. Copyright © 2016. Published by Elsevier B.V.

Modification of natural matrix lac-bagasse for matrix composite films

NASA Astrophysics Data System (ADS)

Nurhayati, Nanik Dwi; Widjaya, Karna; Triyono

2016-02-01

Material technology continues to be developed in order to a material that is more efficient with composite technology is a combination of two or more materials to obtain the desired material properties. The objective of this research was to modification and characterize the natural matrix lac-bagasse as composite films. The first step, natural matrix lac was changed from solid to liquid using an ethanol as a solvent so the matrix homogenly. Natural matrix lac was modified by adding citric acid with concentration variation. Secondly, the bagasse delignification using acid hydrolysis method. The composite films natural matrix lac-bagasse were prepared with optimum modified the addition citric acid 5% (v/v) and delignification bagasse optimum at 1,5% (v/v) in hot press at 80°C 6 Kg/cm-1. Thirdly, composite films without and with modification were characterized functional group analysis using FTIR spectrophotometer and mechanical properties using Universal Testing Machine. The result of research showed natural matrix lac can be modified by reaction with citric acid. FTIR spectra showed without and with modification had functional groups wide absorption 3448 cm-1 group -OH, C=O ester strong on 1712 cm-1 and the methylene group -CH2 on absorption 1465 cm-1. The mechanical properties showed tensile strength 0,55 MPa and elongation at break of 0,95 %. So that composite films natural matrix lac can be made with reinforcement bagasse for material application.

Evaluation of Polymerase Chain Reaction for Detecting Coliform Bacteria in Drinking Water Sources

PubMed Central

Isfahani, Bahram Nasr; Fazeli, Hossein; Babaie, Zeinab; Poursina, Farkhondeh; Moghim, Sharareh; Rouzbahani, Meisam

2017-01-01

Background: Coliform bacteria are used as indicator organisms for detecting fecal pollution in water. Traditional methods including microbial culture tests in lactose-containing media and enzyme-based tests for the detection of β-galactosidase; however, these methods are time-consuming and less specific. The aim of this study was to evaluate polymerase chain reaction (PCR) for detecting coliform. Materials and Methods: Totally, 100 of water samples from Isfahan drinking water source were collected. Coliform bacteria and Escherichia coli were detected in drinking water using LacZ and LamB genes in PCR method performed in comparison with biochemical tests for all samples. Results: Using phenotyping, 80 coliform isolates were found. The results of the biochemical tests illustrated 78.7% coliform bacteria and 21.2% E. coli. PCR results for LacZ and LamB genes were 67.5% and 17.5%, respectively. Conclusion: The PCR method was shown to be an effective, sensitive, and rapid method for detecting coliform and E. coli in drinking water from the Isfahan drinking water sources. PMID:29142893

SABE Colombia: Survey on Health, Well-Being, and Aging in Colombia—Study Design and Protocol

PubMed Central

Corchuelo, Jairo; Curcio, Carmen-Lucia; Calzada, Maria-Teresa; Mendez, Fabian

2016-01-01

Objective. To describe the design of the SABE Colombia study. The major health study of the old people in Latin America and the Caribbean (LAC) is the Survey on Health, Well-Being, and Aging in LAC, SABE (from initials in Spanish: SAlud, Bienestar & Envejecimiento). Methods. The SABE Colombia is a population-based cross-sectional study on health, aging, and well-being of elderly individuals aged at least 60 years focusing attention on social determinants of health inequities. Methods and design were similar to original LAC SABE. The total sample size of the study at the urban and rural research sites (244 municipalities) was 23.694 elderly Colombians representative of the total population. The study had three components: (1) a questionnaire covering active aging determinants including anthropometry, blood pressure measurement, physical function, and biochemical and hematological measures; (2) a subsample survey among family caregivers; (3) a qualitative study with gender and cultural perspectives of quality of life to understand different dimensions of people meanings. Conclusions. The SABE Colombia is a comprehensive, multidisciplinary study of the elderly with respect to active aging determinants. The results of this study are intended to inform public policies aimed at tackling health inequalities for the aging society in Colombia. PMID:27956896

Evaluating the Outcomes of a School Based Theraplay® Project for Looked after Children

ERIC Educational Resources Information Center

Francis, Yvonne J.; Bennion, Kim; Humrich, Sarah

2017-01-01

Research shows that Looked After Children (LAC) may experience emotional instability which can reduce their capacity to engage with education. This study evaluates an attachment based therapeutic Theraplay® intervention designed to bridge the gap between the emotional well-being of LAC and their engagement in education. Twenty LAC between the ages…

Impact of cannabis sativa (marijuana) smoke on alveolar bone loss: a histometric study in rats.

PubMed

Nogueira-Filho, Getulio R; Todescan, Sylvia; Shah, Adnan; Rosa, Bruno T; Tunes, Urbino da R; Cesar Neto, Joao B

2011-11-01

Cannabis sativa (marijuana) can interfere with bone physiopathology because of its effect on osteoblast and osteoclast activity. However, its impact on periodontal tissues is still controversial. The present study evaluates whether marijuana smoke affects bone loss (BL) on ligature-induced periodontitis in rats. Thirty male Wistar rats were used in the study. A ligature was placed around one of the mandible first molars (ligated teeth) of each animal, and they were then randomly assigned to one of two groups: control (n = 15) or marijuana smoke inhalation ([MSI] for 8 minutes per day; n = 15). Urine samples were obtained to detect the presence of tetrahydrocannabinol. After 30 days, the animals were sacrificed and decalcified sections of the furcation area were obtained and evaluated according to the following histometric parameters: bone area (BA), bone density (BD), and BL. Tetrahydrocannabinol was positive in urine samples only for the rats of the MSI group. Non-significant differences were observed for unligated teeth from both groups regarding BL, BA, and BD (P >0.05). However, intragroup analysis showed that all ligated teeth presented BL and a lower BA and BD compared to unligated teeth (P <0.05). The intergroup evaluation of the ligated teeth showed that the MSI group presented higher BL and lower BD (P <0.05) compared to ligated teeth from the control group. Considering the limitations of this animal study, cannabis smoke may impact alveolar bone by increasing BL resulting from ligature-induced periodontitis.

Overexpression of a novel thermostable and chloride-tolerant laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and its application in synthetic dye decolorization.

PubMed

Liu, Huiping; Cheng, Yu; Du, Bing; Tong, Chaofan; Liang, Shuli; Han, Shuangyan; Zheng, Suiping; Lin, Ying

2015-01-01

Laccases have been used for the decolorization and detoxification of synthetic dyes due to their ability to oxidize a wide variety of dyes with water as the sole byproduct. A putative laccase gene (LacTT) from Thermus thermophilus SG0.5JP17-16 was screened using the genome mining approach, and it was highly expressed in Pichia pastoris, yielding a high laccase activity of 6130 U/L in a 10-L fermentor. The LacTT open reading frame encoded a protein of 466 amino acid residues with four putative Cu-binding regions. The optimal pH of the recombinant LacTT was 4.5, 6.0, 7.5 and 8.0 with 2,2'-azino-bis(3-ethylbenzothazoline-6-sulfonic acid) (ABTS), syringaldazine (SGZ), guaiacol, and 2,6-dimethoxyphenol (2,6-DMP) as the substrate, respectively. The optimal temperature of LacTT was 90°C with guaiacol as the substrate. LacTT was highly stable at pH 4.0-11.0 and thermostable at 40°C-90°C, confirming that it is a pH-stable and thermostable laccase. Furthermore, LacTT also exhibited high tolerance to halides such as NaCl, NaBr and NaF, and decolorized 100%, 94%, 94% and 73% of Congo Red, Reactive Black B and Reactive Black WNN, and Remazol Brilliant Blue R, respectively. Interestingly, addition of high concentration of NaCl increased the RBBR decolorization efficiency of LacTT. These results suggest that LacTT is a good candidate for industrial applications such as dyestuff processing and degradation of dyes in textile wastewaters.

Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization

PubMed Central

Liu, Huiping; Cheng, Yu; Du, Bing; Tong, Chaofan; Liang, Shuli; Han, Shuangyan; Zheng, Suiping; Lin, Ying

2015-01-01

Laccases have been used for the decolorization and detoxification of synthetic dyes due to their ability to oxidize a wide variety of dyes with water as the sole byproduct. A putative laccase gene (LacTT) from Thermus thermophilus SG0.5JP17-16 was screened using the genome mining approach, and it was highly expressed in Pichia pastoris, yielding a high laccase activity of 6130 U/L in a 10-L fermentor. The LacTT open reading frame encoded a protein of 466 amino acid residues with four putative Cu-binding regions. The optimal pH of the recombinant LacTT was 4.5, 6.0, 7.5 and 8.0 with 2,2'-azino-bis(3-ethylbenzothazoline-6-sulfonic acid) (ABTS), syringaldazine (SGZ), guaiacol, and 2,6-dimethoxyphenol (2,6-DMP) as the substrate, respectively. The optimal temperature of LacTT was 90°C with guaiacol as the substrate. LacTT was highly stable at pH 4.0–11.0 and thermostable at 40°C–90°C, confirming that it is a pH-stable and thermostable laccase. Furthermore, LacTT also exhibited high tolerance to halides such as NaCl, NaBr and NaF, and decolorized 100%, 94%, 94% and 73% of Congo Red, Reactive Black B and Reactive Black WNN, and Remazol Brilliant Blue R, respectively. Interestingly, addition of high concentration of NaCl increased the RBBR decolorization efficiency of LacTT. These results suggest that LacTT is a good candidate for industrial applications such as dyestuff processing and degradation of dyes in textile wastewaters. PMID:25790466

Degradation of dyes using crude extract and a thermostable and pH-stable laccase isolated from Pleurotus nebrodensis.

PubMed

Yuan, Xianghe; Tian, Guoting; Zhao, Yongchang; Zhao, Liyan; Wang, Hexiang; Ng, Tzi Bun

2016-08-01

Three laccase isoenzymes (Lac1, Lac2 and Lac3) have been purified to homogeneity from Pleurotus nebrodensis in our previous study. Lac2 was shown to be the dominant isoform, capable of oxidizing the majority of laccase substrates and manifesting good thermostability and pH stability. Hence, Lac2 was selected to decolourize structurally different dyes and the colour removal efficiencies of Lac2 and the crude extract of P. nebrodensis were compared. By monitoring the λmax of the reaction system during the course of biotransformation, clear hypsochromic shifts were observed for most of the dyes examined, illustrating that at least one peak disappeared as a result of laccase treatment. In general, Lac2 was more efficient within a short time (1 h) and the crude extract, in general, could achieve similar or even higher efficiency when the duration of treatment was extended to 24 h. Malachite green (MG) was chosen to study the detoxifying potential of Lac2, because of the relatively simple structure and high toxicity of the dye towards microorganisms. The toxicity of MG towards both bacteria (Bacillus subtilis, Bacillus licheniformis, Pseudomonas fluorescens and Escherichia coli) and fungi (Fusarium graminearum and Trichoderma harzianum) was dramatically decreased and the potential mechanism was estimated by GC-MS as to remove four methyl groups firstly and the two newly formed amine groups would be degraded or polymerized further. The present study facilitates an understanding of the application of P. nebrodensis laccases and furnishes evidence for the safety of their utilization in the treatment of wastewater emanating from textile industries. © 2016 The Author(s).

Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators

PubMed Central

Loi, Martina; Fanelli, Francesca; Zucca, Paolo; Liuzzi, Vania C.; Quintieri, Laura; Cimmarusti, Maria T.; Monaci, Linda; Haidukowski, Miriam; Logrieco, Antonio F.; Sanjust, Enrico; Mulè, Giuseppina

2016-01-01

Laccases (LCs) are multicopper oxidases that find application as versatile biocatalysts for the green bioremediation of environmental pollutants and xenobiotics. In this study we elucidate the degrading activity of Lac2 pure enzyme form Pleurotus pulmonarius towards aflatoxin B1 (AFB1) and M1 (AFM1). LC enzyme was purified using three chromatographic steps and identified as Lac2 through zymogram and LC-MS/MS. The degradation assays were performed in vitro at 25 °C for 72 h in buffer solution. AFB1 degradation by Lac2 direct oxidation was 23%. Toxin degradation was also investigated in the presence of three redox mediators, (2,2′-azino-bis-[3-ethylbenzothiazoline-6-sulfonic acid]) (ABTS) and two naturally-occurring phenols, acetosyringone (AS) and syringaldehyde (SA). The direct effect of the enzyme and the mediated action of Lac2 with redox mediators univocally proved the correlation between Lac2 activity and aflatoxins degradation. The degradation of AFB1 was enhanced by the addition of all mediators at 10 mM, with AS being the most effective (90% of degradation). AFM1 was completely degraded by Lac2 with all mediators at 10 mM. The novelty of this study relies on the identification of a pure enzyme as capable of degrading AFB1 and, for the first time, AFM1, and on the evidence that the mechanism of an effective degradation occurs via the mediation of natural phenolic compounds. These results opened new perspective for Lac2 application in the food and feed supply chains as a biotransforming agent of AFB1 and AFM1. PMID:27563923

Degradation of dyes using crude extract and a thermostable and pH-stable laccase isolated from Pleurotus nebrodensis

PubMed Central

Yuan, Xianghe; Tian, Guoting; Zhao, Yongchang; Zhao, Liyan; Wang, Hexiang; Ng, Tzi Bun

2016-01-01

Three laccase isoenzymes (Lac1, Lac2 and Lac3) have been purified to homogeneity from Pleurotus nebrodensis in our previous study. Lac2 was shown to be the dominant isoform, capable of oxidizing the majority of laccase substrates and manifesting good thermostability and pH stability. Hence, Lac2 was selected to decolourize structurally different dyes and the colour removal efficiencies of Lac2 and the crude extract of P. nebrodensis were compared. By monitoring the λmax of the reaction system during the course of biotransformation, clear hypsochromic shifts were observed for most of the dyes examined, illustrating that at least one peak disappeared as a result of laccase treatment. In general, Lac2 was more efficient within a short time (1 h) and the crude extract, in general, could achieve similar or even higher efficiency when the duration of treatment was extended to 24 h. Malachite green (MG) was chosen to study the detoxifying potential of Lac2, because of the relatively simple structure and high toxicity of the dye towards microorganisms. The toxicity of MG towards both bacteria (Bacillus subtilis, Bacillus licheniformis, Pseudomonas fluorescens and Escherichia coli) and fungi (Fusarium graminearum and Trichoderma harzianum) was dramatically decreased and the potential mechanism was estimated by GC–MS as to remove four methyl groups firstly and the two newly formed amine groups would be degraded or polymerized further. The present study facilitates an understanding of the application of P. nebrodensis laccases and furnishes evidence for the safety of their utilization in the treatment of wastewater emanating from textile industries. PMID:27354563

A Monte Carlo Approach to Magnetar-powered Transients. II. Broad-lined Type Ic Supernovae Not Associated with GRBs

NASA Astrophysics Data System (ADS)

Wang, L. J.; Cano, Z.; Wang, S. Q.; Zheng, W. K.; Liu, L. D.; Deng, J. S.; Yu, H.; Dai, Z. G.; Han, Y. H.; Xu, D.; Qiu, Y. L.; Wei, J. Y.; Li, B.; Song, L. M.

2017-12-01

Broad-lined type Ic supernovae (SNe Ic-BL) are a subclass of rare core-collapse SNe whose energy source is debated in the literature. Recently, a series of investigations on SNe Ic-BL with the magnetar (plus 56Ni) model were carried out. Evidence for magnetar formation was found for the well-observed SNe Ic-BL 1998bw and 2002ap. In this paper, we systematically study a large sample of SNe Ic-BL not associated with gamma-ray bursts (GRBs). We use photospheric velocity data determined in a homogeneous way. We find that the magnetar+56Ni model provides a good description of the light curves and velocity evolution of our sample of SNe Ic-BL, although some SNe (not all) can also be described by the pure-magnetar model or by the two-component pure-56Ni model (three out of 12 are unlikely to be explained by two-component model). In the magnetar+56Ni model, the amount of 56Ni required to explain their luminosity is significantly reduced, and the derived initial explosion energy is, in general, in accordance with neutrino heating. Some correlations between different physical parameters are evaluated, and their implications regarding magnetic field amplification and the total energy reservoir are discussed.

Spatial and Temporal Variations in Diatoms from la Chaine des Lacs Urban Watershed, Nord-Pas France, in Relation to Water Quality

NASA Astrophysics Data System (ADS)

Noble, P. J.; Van de Vijver, B.; Verleyen, E.; Prygiel, J.; Ivanovsky, A.; Lesven, L.; Billon, G.

2016-12-01

Diatom analysis was conducted on lake sediments in la Chaîne des Lacs (CDL), a shallow eutrophic urban park and storm control system in Villeneuve d'Ascq, France, to address both the present day water quality, and the evolution of this urban system over its 40 year history. The main lake, Lac du Héron (LDH), received recent attention because of water quality problems, including eutrophication, harmful algal blooms, and invasion by the macrophyte Elodea in 2012. A total of 17 sites were collected in CDL, 11 of which were in LDH, to document spatial variability, and a 26cm long core addresses historical changes. The bulk of the diatom assemblage in LDH can be classified as both eutrophic and moderately metal tolerant, using modern national diatom indices developed and used by the French regional water agencies. Surface sediment samples within LDH show large spatial variations in %Cocconeis placentula whose habitat is epiphytic growth on Elodea. Other variation is reflected in the phytoplankton composition both spatially, and interannually. Aulacoseira muzzanensis and Cyclostephanos dubius showed greater abundance in the open water habitats in LDH, whereas sites in CDL outside of LDH had greater Cyclotella meneghiniana. Temporally, Stephanodicsus (largely S. hantzschii), the dominant diatom in early spring, were present in greater abundances in the 2016 surface sediment samples than in any of the 2015 samples. One possible explanation is that the 2016 samples, taken March 30th, preferentially preserved the early spring Stephanodiscus bloom, in contrast to the 2015 samples, which were taken in January. The sediment core provides an historical record, where the uppermost 4cm plot with the bulk of the LDH surface samples and contain abundant Cocconeis, 4 -14cm is phytoplankton-rich, largely Cyclostephanos dubius and Aulacoseira muzzanensis, and represents a less weed-choked environment prior to the 2012 Elodea invasion. The base of the core is dominated by Amphora and Rhoicosphenia abbreviata, is most similar to an outlier site on the Marque River, and represents early conditions after the reservoir was established. Continued work will focus on relating these results to urban development, improvements of the sewage system, and meteorological patterns.

LAC indicators: an evaluation of progress and list of proposed indicators

Treesearch

Alan E. Watson; David N. Cole

1992-01-01

One of the most critical, and difficult, steps in the Limits of Acceptable Change (LAC) process is the selection of indicators. To help with this step, this paper (I) briefly reviews some desirable characteristics of indicators and (2) lists indicators that have been proposed or adopted in LAC plans. From a comparison of this list of indicators and desirable...

Historical development of limits of acceptable change: conceptual clarifications and possible extensions

Treesearch

David N. Cole; George H. Stankey

1997-01-01

The Limits of Acceptable Change (LAC) process was developed to deal with the issue of recreational carrying capacity. For that purpose, the LAC process sought to explicitly define a compromise between resource/visitor experience protection and recreation use goals. The most critical and unique element of the process is the specification of LAC standards that define...

An Actor-Network Theory Reading of Change for Children in Public Care

ERIC Educational Resources Information Center

Parker, Elisabeth

2017-01-01

The education of children in public, or Local Authority (LA), care, known in the United Kingdom (UK) as looked-after children (LAC), is supported by government initiatives to reduce the attainment gap that exists between LAC and their non-LAC peers. These children often find remaining in education a challenge, are twice as likely to be permanently…

Lac Télé structure, Republic of Congo: Geological setting of a cryptozoological and biodiversity hotspot, and evidence against an impact origin

NASA Astrophysics Data System (ADS)

Master, Sharad

2010-11-01

Lac Télé is a large lake, ˜5.6 km in diameter, with an ovoid shape, situated at 17°10'E, 1°20'N, in the great tropical rain forest region of the Republic of Congo. This lake has attracted widespread attention, mainly because of the legends among the local people that it harbours a strange animal known as the Mokele-Mbembe, but also because it is situated in a region that is a hotbed of biodiversity and conservation efforts with respect to various endangered mammalian species, including gorillas and chimpanzees. Because of its appearance, Lac Télé has been regarded as a possible meteorite impact structure. Various expeditions, studying cryptozoology, conservation ecology, biodiversity, and the impact hypothesis, have visited Lac Télé in the past several decades. The Lac Télé structure is located in the NW part of the intracratonic Congo Basin, in a region dominated by Holocene alluvium, dense tropical rain forest, and swamps which form part of the basin of the Likouala aux Herbes, a multi-branched meandering river flowing over very low gradients into the Sangha river, a major tributary of the Congo river. Previous bathymetric studies have shown that the average depth of Lac Télé is only 4 m, including organic-rich silty sediments. The structure is that of a flat-bottomed dish. Modelling of the Lac Télé as an impact structure indicates a number of features which ought to be present. The absence of any of these features, coupled with the irregular ovoid shape, the palynological record, and the location of the structure at the intersection of major regional lineaments, is regarded as evidence against the impact hypothesis. Lac Télé as an isolated lake ecosystem is not unique in the Congo Basin, and there are several other similar small shallow isolated lakes surrounded by rain forest and marshes, some of which formed by damming of drainage systems by neotectonic faults. It is suggested that the formation of Lac Télé may be related to its location over neotectonically reactivated regional lineaments, which are also seismically active. Lac Télé and other similar hydrologic systems may be biodiversity hotspots because they acted as refugia following neotectonic hydrological re-organization of the Congo Basin.

Discovery of high and very high-energy emission from the BL Lacertae object SHBL J001355.9-185406

NASA Astrophysics Data System (ADS)

H.E.S.S. Collaboration; Abramowski, A.; Acero, F.; Aharonian, F.; Akhperjanian, A. G.; Angüner, E.; Anton, G.; Balenderan, S.; Balzer, A.; Barnacka, A.; Becherini, Y.; Becker Tjus, J.; Bernlöhr, K.; Birsin, E.; Bissaldi, E.; Biteau, J.; Boisson, C.; Bolmont, J.; Bordas, P.; Brucker, J.; Brun, F.; Brun, P.; Bulik, T.; Carrigan, S.; Casanova, S.; Cerruti, M.; Chadwick, P. M.; Chalme-Calvet, R.; Chaves, R. C. G.; Cheesebrough, A.; Chrétien, M.; Colafrancesco, S.; Cologna, G.; Conrad, J.; Couturier, C.; Dalton, M.; Daniel, M. K.; Davids, I. D.; Degrange, B.; Deil, C.; deWilt, P.; Dickinson, H. J.; Djannati-Ataï, A.; Domainko, W.; O'C. Drury, L.; Dubus, G.; Dutson, K.; Dyks, J.; Dyrda, M.; Edwards, T.; Egberts, K.; Eger, P.; Espigat, P.; Farnier, C.; Fegan, S.; Feinstein, F.; Fernandes, M. V.; Fernandez, D.; Fiasson, A.; Fontaine, G.; Förster, A.; Füßling, M.; Gajdus, M.; Gallant, Y. A.; Garrigoux, T.; Gast, H.; Giebels, B.; Glicenstein, J. F.; Göring, D.; Grondin, M.-H.; Grudzińska, M.; Häffner, S.; Hague, J. D.; Hahn, J.; Harris, J.; Heinzelmann, G.; Henri, G.; Hermann, G.; Hervet, O.; Hillert, A.; Hinton, J. A.; Hofmann, W.; Hofverberg, P.; Holler, M.; Horns, D.; Jacholkowska, A.; Jahn, C.; Jamrozy, M.; Janiak, M.; Jankowsky, F.; Jung, I.; Kastendieck, M. A.; Katarzyński, K.; Katz, U.; Kaufmann, S.; Khélifi, B.; Kieffer, M.; Klepser, S.; Klochkov, D.; Kluźniak, W.; Kneiske, T.; Kolitzus, D.; Komin, Nu.; Kosack, K.; Krakau, S.; Krayzel, F.; Krüger, P. P.; Laffon, H.; Lamanna, G.; Lefaucheur, J.; Lemoine-Goumard, M.; Lenain, J.-P.; Lennarz, D.; Lohse, T.; Lopatin, A.; Lu, C.-C.; Marandon, V.; Marcowith, A.; Maurin, G.; Maxted, N.; Mayer, M.; McComb, T. J. L.; Medina, M. C.; Méhault, J.; Menzler, U.; Meyer, M.; Moderski, R.; Mohamed, M.; Moulin, E.; Murach, T.; Naumann, C. L.; de Naurois, M.; Nedbal, D.; Niemiec, J.; Nolan, S. J.; Oakes, L.; Ohm, S.; de Oña Wilhelmi, E.; Opitz, B.; Ostrowski, M.; Oya, I.; Panter, M.; Parsons, R. D.; Paz Arribas, M.; Pekeur, N. W.; Pelletier, G.; Perez, J.; Petrucci, P.-O.; Peyaud, B.; Pita, S.; Poon, H.; Pühlhofer, G.; Punch, M.; Quirrenbach, A.; Raab, S.; Raue, M.; Reimer, A.; Reimer, O.; Renaud, M.; de los Reyes, R.; Rieger, F.; Rob, L.; Rosier-Lees, S.; Rowell, G.; Rudak, B.; Rulten, C. B.; Sahakian, V.; Sanchez, D. A.; Santangelo, A.; Schlickeiser, R.; Schüssler, F.; Schulz, A.; Schwanke, U.; Schwarzburg, S.; Schwemmer, S.; Sol, H.; Spengler, G.; Spieß, F.; Stawarz, Ł.; Steenkamp, R.; Stegmann, C.; Stinzing, F.; Stycz, K.; Sushch, I.; Szostek, A.; Tavernet, J.-P.; Terrier, R.; Tluczykont, M.; Trichard, C.; Valerius, K.; van Eldik, C.; Vasileiadis, G.; Venter, C.; Viana, A.; Vincent, P.; Völk, H. J.; Volpe, F.; Vorster, M.; Wagner, S. J.; Wagner, P.; Ward, M.; Weidinger, M.; White, R.; Wierzcholska, A.; Willmann, P.; Wörnlein, A.; Wouters, D.; Zacharias, M.; Zajczyk, A.; Zdziarski, A. A.; Zech, A.; Zechlin, H.-S.

2013-06-01

The detection of the high-frequency peaked BL Lac object (HBL) SHBL J001355.9-185406 (z = 0.095) at high (HE; 100 MeV < E < 300 GeV) and very high-energy (VHE; E > 100 GeV) with the Fermi Large Area Telescope (LAT) and the High Energy Stereoscopic System (H.E.S.S.) is reported. Dedicated observations were performed with the H.E.S.S. telescopes, leading to a detection at the 5.5σ significance level. The measured flux above 310 GeV is (8.3 ± 1.7stat ± 1.7sys) × 10-13 photons cm-2 s-1 (about 0.6% of that of the Crab Nebula), and the power-law spectrum has a photon index of Γ = 3.4 ± 0.5stat ± 0.2sys. Using 3.5 years of publicly available Fermi-LAT data, a faint counterpart has been detected in the LAT data at the 5.5σ significance level, with an integrated flux above 300 MeV of (9.3 ± 3.4stat ± 0.8sys) × 10-10 photons cm-2 s-1 and a photon index of Γ = 1.96 ± 0.20stat ± 0.08sys. X-ray observations with Swift-XRT allow the synchrotron peak energy in νFν representation to be located at ~1.0 keV. The broadband spectral energy distribution is modelled with a one-zone synchrotronself-Compton (SSC) model and the optical data by a black-body emission describing the thermal emission of the host galaxy. The derived parameters are typical of HBLs detected at VHE, with a particle-dominated jet.

Evaluation of an in vitro toxicogenetic mouse model for hepatotoxicity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martinez, Stephanie M.; Bradford, Blair U.; Soldatow, Valerie Y.

2010-12-15

Numerous studies support the fact that a genetically diverse mouse population may be useful as an animal model to understand and predict toxicity in humans. We hypothesized that cultures of hepatocytes obtained from a large panel of inbred mouse strains can produce data indicative of inter-individual differences in in vivo responses to hepato-toxicants. In order to test this hypothesis and establish whether in vitro studies using cultured hepatocytes from genetically distinct mouse strains are feasible, we aimed to determine whether viable cells may be isolated from different mouse inbred strains, evaluate the reproducibility of cell yield, viability and functionality overmore » subsequent isolations, and assess the utility of the model for toxicity screening. Hepatocytes were isolated from 15 strains of mice (A/J, B6C3F1, BALB/cJ, C3H/HeJ, C57BL/6J, CAST/EiJ, DBA/2J, FVB/NJ, BALB/cByJ, AKR/J, MRL/MpJ, NOD/LtJ, NZW/LacJ, PWD/PhJ and WSB/EiJ males) and cultured for up to 7 days in traditional 2-dimensional culture. Cells from B6C3F1, C57BL/6J, and NOD/LtJ strains were treated with acetaminophen, WY-14,643 or rifampin and concentration-response effects on viability and function were established. Our data suggest that high yield and viability can be achieved across a panel of strains. Cell function and expression of key liver-specific genes of hepatocytes isolated from different strains and cultured under standardized conditions are comparable. Strain-specific responses to toxicant exposure have been observed in cultured hepatocytes and these experiments open new opportunities for further developments of in vitro models of hepatotoxicity in a genetically diverse population.« less

Discovery of high and very high-energy emission from the BL Lacertae object SHBL J001355.9–185406

DOE PAGES

Abramowski, A.; Acero, F.; Aharonian, F.; ...

2013-06-05

In this paper, the detection of the high-frequency peaked BL Lac object (HBL) SHBL J001355.9–185406 (z = 0.095) at high (HE; 100 MeV < E < 300 GeV) and very high-energy (VHE; E > 100 GeV) with the Fermi Large Area Telescope (LAT) and the High Energy Stereoscopic System (H.E.S.S.) is reported. Dedicated observations were performed with the H.E.S.S. telescopes, leading to a detection at the 5.5σ significance level. The measured flux above 310 GeV is (8.3 ± 1.7 stat ± 1.7 sys) × 10 -13 photons cm -2 s -1 (about 0.6% of that of the Crab Nebula), andmore » the power-law spectrum has a photon index of Γ = 3.4 ± 0.5 stat ± 0.2 sys. Using 3.5 years of publicly available Fermi-LAT data, a faint counterpart has been detected in the LAT data at the 5.5σ significance level, with an integrated flux above 300 MeV of (9.3 ± 3.4 stat ± 0.8 sys) × 10 -10 photons cm -2 s -1 and a photon index of Γ = 1.96 ± 0.20 stat ± 0.08 sys. X-ray observations with Swift-XRT allow the synchrotron peak energy in νF ν representation to be located at ~1.0 keV. The broadband spectral energy distribution is modelled with a one-zone synchrotronself-Compton (SSC) model and the optical data by a black-body emission describing the thermal emission of the host galaxy. Lastly, the derived parameters are typical of HBLs detected at VHE, with a particle-dominated jet.« less

Sunflower (Helianthus annuus) long-chain acyl-coenzyme A synthetases expressed at high levels in developing seeds.

PubMed

Aznar-Moreno, Jose A; Venegas Calerón, Mónica; Martínez-Force, Enrique; Garcés, Rafael; Mullen, Robert; Gidda, Satinder K; Salas, Joaquín J

2014-03-01

Long chain fatty acid synthetases (LACSs) activate the fatty acid chains produced by plastidial de novo biosynthesis to generate acyl-CoA derivatives, important intermediates in lipid metabolism. Oilseeds, like sunflower, accumulate high levels of triacylglycerols (TAGs) in their seeds to nourish the embryo during germination. This requires that sunflower seed endosperm supports very active glycerolipid synthesis during development. Sunflower seed plastids produce large amounts of fatty acids, which must be activated through the action of LACSs, in order to be incorporated into TAGs. We cloned two different LACS genes from developing sunflower endosperm, HaLACS1 and HaLACS2, which displayed sequence homology with Arabidopsis LACS9 and LACS8 genes, respectively. These genes were expressed at high levels in developing seeds and exhibited distinct subcellular distributions. We generated constructs in which these proteins were fused to green fluorescent protein and performed transient expression experiments in tobacco cells. The HaLACS1 protein associated with the external envelope of tobacco chloroplasts, whereas HaLACS2 was strongly bound to the endoplasmic reticulum. Finally, both proteins were overexpressed in Escherichia coli and recovered as active enzymes in the bacterial membranes. Both enzymes displayed similar substrate specificities, with a very high preference for oleic acid and weaker activity toward stearic acid. On the basis of our findings, we discuss the role of these enzymes in sunflower oil synthesis. © 2013 Scandinavian Plant Physiology Society.

Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma.

PubMed

Cheng, Zhenshun; Tan, Qiuyue; Tan, Weijun; Zhang, L I

2015-08-01

The aim of the present study was to determine the effect of cigarette smoke on the expression of Notch proteins in lung adenocarcinoma (LAC). Protein expression levels of Notch1 and Notch3 were analyzed using immunohistochemistry in 102 human LAC specimens. Of these, 52 were obtained from smokers and 50 from non-smokers. In addition, cigarette smoke extract (CSE) at varying concentrations (1, 2.5 and 5%) was administered to A549 cells. The expression of Notch1 and Notch3 protein was then detected by western blot analysis at different time points (0, 8, 24 and 48 h). Of the 102 LAC specimens, 42 (41.2%) were positive for Notch1 and 63 (61.8%) were positive for Notch3. There was no significant difference in the level of Notch1 expression between smokers and non-smokers with LAC (P>0.05). The positive rate and staining intensity of Notch3 expression were increased in the smokers compared with the non-smokers (P<0.05). The expression of Notch3 protein in A549 cells increased in a time- and dose-dependent manner following treatment with CSE, whilst the expression of Notch1 protein appeared stable. The results suggested that cigarette smoke was able to induce the expression of Notch3, not Notch1, protein in LAC. The data revealed an upregulation of Notch3 in LAC following cigarette smoke exposure. Such findings may provide a novel therapeutic target for the treatment of LAC.

Development of multiplex PCR for the detection of total coliform bacteria for Escherichia coli and Clostridium perfringens in drinking water.

PubMed

Tantawiwat, Suwalee; Tansuphasiri, Unchalee; Wongwit, Waranya; Wongchotigul, Varee; Kitayaporn, Dwip

2005-01-01

Multiplex PCR amplification of lacZ, uidA and plc genes was developed for the simultaneous detection of total coliform bacteria for Escherichia coli and Clostridium perfringens, in drinking water. Detection by agarose gel electrophoresis yielded a band of 876 bp for the lacZ gene of all coliform bacteria; a band of 147 bp for the uidA gene and a band of 876 bp for the lacZ gene of all strains of E. coli; a band of 280 bp for the p/c gene for all strains of C. perfringens; and a negative result for all three genes when tested with other bacteria. The detection limit was 100 pg for E. coli and C. perfringens, and 1 ng for coliform bacteria when measured with purified DNA. This assay was applied to the detection of these bacteria in spiked water samples. Spiked water samples with 0-1,000 CFU/ml of coliform bacteria and/or E. coli and/or C. perfringens were detected by this multiplex PCR after a pre-enrichment step to increase the sensitivity and to ensure that the detection was based on the presence of cultivable bacteria. The result of bacterial detection from the multiplex PCR was comparable with that of a standard plate count on selective medium (p=0.62). When using standard plate counts as a gold standard, the sensitivity for this test was 99.1% (95% CI 95.33, 99.98) and the specificity was 90.9 % (95% CI 75.67, 98.08). Multiplex PCR amplification with a pre-enrichment step was shown to be an effective, sensitive and rapid method for the simultaneous detection of these three microbiological parameters in drinking water.

Water in the Cratonic Mantle: Insights from FTIR Data on Lac De Gras Xenoliths (Slave Craton, Canada)

NASA Technical Reports Server (NTRS)

Peslier, Anne H.; Brandon, Alan D.; Schaffer, Lillian Aurora; O'Reilly, Suzanne Yvette; Griffin, William L.; Morris, Richard V.; Graff, Trevor G.; Agresti, David G.

2014-01-01

The mantle lithosphere beneath the cratonic part of continents is the deepest (> 200 km) and oldest (>2-3 Ga) on Earth, remaining a conundrum as to how these cratonic roots could have resisted delamination by asthenospheric convection over time. Water, or trace H incorporated in mineral defects, could be a key player in the evolution of continental lithosphere because it influences melting and rheology of the mantle. Mantle xenoliths from the Lac de Gras kimberlite in the Slave craton were analyzed by FTIR. The cratonic mantle beneath Lac de Gras is stratified with shallow (<145 km) oxidized ultradepleted peridotites and pyroxenites with evidence for carbonatitic metasomatism, underlain by reduced and less depleted peridotites metasomatized by kimberlite melts. Peridotites analyzed so far have H O contents in ppm weight of 7-100 in their olivines, 58 to 255 in their orthopyroxenes (opx), 11 to 84 in their garnet, and 139 in one clinopyroxene. A pyroxenite contains 58 ppm H2O in opx and 5 ppm H2O in its olivine and garnet. Olivine and garnet from the deep peridotites have a range of water contents extending to higher values than those from the shallow ones. The FTIR spectra of olivines from the shallow samples have more prominent Group II OH bands compared to the olivines from the deep samples, consistent with a more oxidized mantle environment. The range of olivine water content is similar to that observed in Kaapvaal craton peridotites at the same depths (129-184 km) but does not extend to as high values as those from Udachnaya (Siberian craton). The Slave, Kaapvaal and Siberian cratons will be compared in terms of water content distribution, controls and role in cratonic root longevity.

1WHSP: An IR-based sample of ~1000 VHE γ -ray blazar candidates

DOE PAGES

Arsioli, B.; Fraga, B.; Giommi, P.; ...

2015-06-23

Context. Blazars are the dominant type of extragalactic sources at microwave and at γ-ray energies. In the most energetic part of the electromagnetic spectrum (E > ≳ 100 GeV) a large fraction of high Galactic latitude sources are blazars of the High Synchrotron Peaked (HSP) type, that is BL Lac objects with synchrotron power peaking in the UV or in the X-ray band. Building new large samples of HSP blazars is key to understand the properties of jets under extreme conditions, and to study the demographics and the peculiar cosmological evolution of these sources. Aims. HSP blazars are remarkably rare,more » with only a few hundreds of them expected to be above the sensitivity limits of currently available surveys, some of which include hundreds of millions of sources. To find these very uncommon objects, we have devised a method that combines ALLWISE survey data with multi-frequency selection criteria. Methods. The sample was defined starting from a primary list of infrared colour-colour selected sources from the ALLWISE all sky survey database, and applying further restrictions on IR-radio and IR-X-ray flux ratios. Using a polynomial fit to the multi-frequency data (radio to X-ray) we estimated synchrotron peak frequencies and fluxes of each object. Results. We assembled a sample including 992 sources, which is currently the largest existing list of confirmed and candidates HSP blazars. All objects are expected to radiate up to the highest γ-ray photon energies. In fact, 299 of these are confirmed emitters of GeV γ-ray photons (based on Fermi-LAT catalogues), and 36 have already been detected in the TeV band. The majority of sources in the sample are within reach of the upcoming Cherenkov Telescope Array (CTA), and many may be detectable even by the current generation of Cherenkov telescopes during flaring episodes. The sample includes 425 previously known blazars, 151 new identifications, and 416 HSP candidates (mostly faint sources) for which no optical spectra is available yet. The full 1WHSP catalogue is on-line at http://www.asdc.asi.it/1whsp/ providing a direct link to the SED building tool where multifrequency data for each source can be easily visualised.« less

Current status and future prospects of an automated sample exchange system PAM for protein crystallography

NASA Astrophysics Data System (ADS)

Hiraki, M.; Yamada, Y.; Chavas, L. M. G.; Matsugaki, N.; Igarashi, N.; Wakatsuki, S.

2013-03-01

To achieve fully-automated and/or remote data collection in high-throughput X-ray experiments, the Structural Biology Research Centre at the Photon Factory (PF) has installed PF automated mounting system (PAM) for sample exchange robots at PF macromolecular crystallography beamlines BL-1A, BL-5A, BL-17A, AR-NW12A and AR-NE3A. We are upgrading the experimental systems, including the PAM for stable and efficient operation. To prevent human error in automated data collection, we installed a two-dimensional barcode reader for identification of the cassettes and sample pins. Because no liquid nitrogen pipeline in the PF experimental hutch is installed, the users commonly add liquid nitrogen using a small Dewar. To address this issue, an automated liquid nitrogen filling system that links a 100-liter tank to the robot Dewar has been installed on the PF macromolecular beamline. Here we describe this new implementation, as well as future prospects.

Macrophage inflammatory protein-1α shows predictive value as a risk marker for subjects and sites vulnerable to bone loss in a longitudinal model of aggressive periodontitis.

PubMed

Fine, Daniel H; Markowitz, Kenneth; Fairlie, Karen; Tischio-Bereski, Debbie; Ferrandiz, Javier; Godboley, Dipti; Furgang, David; Gunsolley, John; Best, Al

2014-01-01

Improved diagnostics remains a fundamental goal of biomedical research. This study was designed to assess cytokine biomarkers that could predict bone loss (BL) in localized aggressive periodontitis. 2,058 adolescents were screened. Two groups of 50 periodontally healthy adolescents were enrolled in the longitudinal study. One group had Aggregatibacter actinomycetemcomitans (Aa), the putative pathogen, while the matched cohort did not. Cytokine levels were assessed in saliva and gingival crevicular fluid (GCF). Participants were sampled, examined, and radiographed every 6 months for 2-3 years. Disease was defined as radiographic evidence of BL. Saliva and GCF was collected at each visit, frozen, and then tested retrospectively after detection of BL. Sixteen subjects with Aa developed BL. Saliva from Aa-positive and Aa-negative healthy subjects was compared to subjects who developed BL. GCF was collected from 16 subjects with BL and from another 38 subjects who remained healthy. GCF from BL sites in the 16 subjects was compared to healthy sites in these same subjects and to healthy sites in subjects who remained healthy. Results showed that cytokines in saliva associated with acute inflammation were elevated in subjects who developed BL (i.e., MIP-1α MIP-1β IL-α, IL-1β and IL-8; p<0.01). MIP-1α was elevated 13-fold, 6 months prior to BL. When MIP-1α levels were set at 40 pg/ml, 98% of healthy sites were below that level (Specificity); whereas, 93% of sites with BL were higher (Sensitivity), with comparable Predictive Values of 98%; p<0.0001; 95% C.I. = 42.5-52.7). MIP-1α consistently showed elevated levels as a biomarker for BL in both saliva and GCF, 6 months prior to BL. MIP-1α continues to demonstrate its strong candidacy as a diagnostic biomarker for both subject and site vulnerability to BL.