Preparative Purification of Polyphenols from Aronia melanocarpa (Chokeberry) with Cellular Antioxidant and Antiproliferative Activity.

PubMed

Gao, Ningxuan; Wang, Yuehua; Jiao, Xinyao; Chou, Shurui; Li, Enhui; Li, Bin

2018-01-10

The aim of this study was the purification process of polyphenols from Aronia melanocarpa (chokeberry), and the purification parameters were optimised by adsorption and desorption tests. By comparing adsorption and desorption ability of polyphenols from chokeberry on six kinds of macroporous resin, XAD-7 resin was selected. Experiments prove that the best purification parameters of static adsorption and desorption were sample pH = 4.0 with 4 h of adsorption; and desorption solvent is 95% ethanol (pH = 7.0) with 2 h of desorption. The best dynamic parameters were 9.3 bed volume (BV) of sample loading amount at a feeding flow rate of 2 BV/h, and washing the column with 5.8 BV of water, followed by subsequent elution with an eluent volume of 5.0 mL at an elution flow rate of 2 BV/h. Next the antioxidant and antiproliferative activity of polyphenols from chokeberry, blueberries, haskap berries was studied on HepG2 human liver cancer cells. The results show that polyphenol from chokeberry has a strong antioxidant effect. Taking into account the content of polyphenols in fruit, polyphenols from chokeberry represent a very valuable natural antioxidant source with antiproliferative products.

Chokeberry (Aronia melanocarpa (Michx.) Elliot) concentrate inhibits NF-κB and synergizes with selenium to inhibit the release of pro-inflammatory mediators in macrophages.

PubMed

Appel, Kurt; Meiser, Peter; Millán, Estrella; Collado, Juan Antonio; Rose, Thorsten; Gras, Claudia C; Carle, Reinhold; Muñoz, Eduardo

2015-09-01

Black chokeberry has been known to play a protective role in human health due to its high polyphenolic content including anthocyanins and caffeic acid derivatives. In the present study, we first characterized the polyphenolic content of a commercial chokeberry concentrate and investigated its effect on LPS-induced NF-κB activation and release of pro-inflammatory mediators in macrophages in the presence or the absence of sodium selenite. Examination of the phytochemical profile of the juice concentrate revealed high content of polyphenols (3.3%), including anthocyanins, proanthocyanidins, phenolic acids, and flavonoids. Among them, cyanidin-3-O-galactoside and caffeoylquinic acids were identified as the major compounds. Data indicated that chokeberry concentrate inhibited both the release of TNFα, IL-6 and IL-8 in human peripheral monocytes and the activation of the NF-κB pathway in RAW 264.7 macrophage cells. Furthermore, chokeberry synergizes with sodium selenite to inhibit NF-κB activation, cytokine release and PGE2 synthesis. These findings suggest that selenium added to chokeberry juice enhances significantly its anti-inflammatory activity, thus revealing a sound approach in order to tune the use of traditional herbals by combining them with micronutrients. Copyright © 2015 Elsevier B.V. All rights reserved.

In vitro antileukaemic activity of extracts from chokeberry (Aronia melanocarpa [Michx] Elliott) and mulberry (Morus alba L.) leaves against sensitive and multidrug resistant HL60 cells.

PubMed

Skupień, Katarzyna; Kostrzewa-Nowak, Dorota; Oszmiański, Jan; Tarasiuk, Jolanta

2008-05-01

The aim of the present study was to determine in vitro antileukaemic activities of extracts obtained from chokeberry (Aronia melanocarpa [Michx] Elliot) and mulberry (Morus alba L.) leaves against promyelocytic HL60 cell line and its multidrug resistant sublines exhibiting two different MDR phenotypes: HL60/VINC (overexpressing P-glycoprotein) and HL60/DOX (overexpressing MRP1 protein). It was found that the extracts from chokeberry and mulberry leaves were active against the sensitive leukaemic cell line HL60 and retained the in vitro activity against multidrug resistant sublines (HL60/VINC and HL60/DOX). The values of resistance factor (RF) found for these extracts were very low lying in the range 1.2-1.6.

Identification and Characterization of Pseudocercospora pyricola Causing Leaf Spots on Aronia melanocarpa.

PubMed

Park, Sung-Hee; Choi, In-Young; Seo, Kyoung-Won; Kim, Jin-Ho; Galea, Victor; Shin, Hyeon-Dong

2017-03-01

Leaf spot disease on black chokeberry ( Aronia melanocarpa ) was observed at several locations in Korea during 2014-2015. Leaf spots were distinct, scattered over the leaf surface and along the leaf border, subcircular to irregular and brown surrounded by a distinct dark color, and were expanded and coalesced into irregularly shaped lesions. Severely infected leaves became dry and fell off eventually. The causative agent was identified as Pseudocercospora pyricola . Morphological observations and phylogenetic analyses of multiple genes, including internal transcribed spacer, translation elongation factor 1-alpha, actin, and the large subunit ribosomal DNA were conducted. The pathogenicity test was conducted twice yielding similar results, fulfilling Koch's postulates. To our knowledge, this is the first report on P. pyricola infection of A. melanocarpa globally.

Determination of chokeberry (Aronia melanocarpa) polyphenol components using liquid chromatography-tandem mass spectrometry: Overall contribution to antioxidant activity.

PubMed

Lee, Ji Eun; Kim, Gon-Sup; Park, Semin; Kim, Yun-Hi; Kim, Man-Bae; Lee, Won Sup; Jeong, Sung Woo; Lee, Soo Jung; Jin, Jong Sung; Shin, Sung Chul

2014-03-01

The type and content of plant polyphenols can be influenced by maturity. Korean chokeberry (Aronia melanocarpa) leaves of three different maturities (young, mature, and aged) were extracted with 70% aqueous methanol. The polyphenols in the leaves were analysed for the first time using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and comparison with reported data. Among the 12 characterised components, five flavonoids, 3, 4, and 10-12, and a dicaffeoylquinic acid derivative, 6, were characterised for the first time in chokeberry leaves. Each polyphenol component was validated and quantified using a representative polyphenol standard of the same group. The antioxidant activity of the three different mature leaf extracts was determined. The antioxidant activity was highest for young leaves, followed by mature and aged leaves. The results suggest that younger chokeberry leaves may be more favourable for processing a higher quality functional tea due to their higher polyphenol content. Copyright © 2013 Elsevier Ltd. All rights reserved.

Identification and Characterization of Pseudocercospora pyricola Causing Leaf Spots on Aronia melanocarpa

PubMed Central

Park, Sung-Hee; Choi, In-Young; Seo, Kyoung-Won; Kim, Jin-Ho; Galea, Victor

2017-01-01

Leaf spot disease on black chokeberry (Aronia melanocarpa) was observed at several locations in Korea during 2014–2015. Leaf spots were distinct, scattered over the leaf surface and along the leaf border, subcircular to irregular and brown surrounded by a distinct dark color, and were expanded and coalesced into irregularly shaped lesions. Severely infected leaves became dry and fell off eventually. The causative agent was identified as Pseudocercospora pyricola. Morphological observations and phylogenetic analyses of multiple genes, including internal transcribed spacer, translation elongation factor 1-alpha, actin, and the large subunit ribosomal DNA were conducted. The pathogenicity test was conducted twice yielding similar results, fulfilling Koch's postulates. To our knowledge, this is the first report on P. pyricola infection of A. melanocarpa globally. PMID:28435353

Influence of juice processing factors on quality of black chokeberry pomace as a future resource for colour extraction.

PubMed

Vagiri, Michael; Jensen, Martin

2017-02-15

Aronia melanocarpa berries are a rich source of anthocyanins and its pomace, a by-product of juice processing, could be efficiently used for extraction of natural colours for the food industry. This study evaluated the influence blanching, freezing, maceration temperatures (2°C, 50°C) and enzyme treatments before juice pressing on the yield and anthocyanin composition of both juice and pomace. Total anthocyanin levels in pomace were affected mostly by enzyme treatment followed by maceration temperature. The pre-heating of the mash prior to processing increased juice yield and retention of anthocyanins in the pomace. Cold maceration of frozen berries without enzyme addition gave the highest concentrations of anthocyanins in the pomace, and both cold and hot maceration of fresh unblanched berries with enzyme the lowest. The results support future exploitation of natural colours from pomace side streams of Aronia, thus increasing competitiveness of Aronia berry production. Copyright © 2016 Elsevier Ltd. All rights reserved.

Polyphenols and Volatile Compounds in Commercial Chokeberry (Aronia melanocarpa) Products.

PubMed

Romani, Annalisa; Vignolini, Pamela; Ieri, Francesca; Heimler, Daniela

2016-01-01

Aronia melanocarpa (Michx.) Elliott commercial products (dried fruit, juice and compote) were analyzed for their polyphenol content by chromatographic and spectrophotometric analyses in order to ascertain the fate of this group of compounds when fresh fruit is processed and sold in different forms on the market. Different classes of polyphenols were investigated: hydroxycinnamic derivatives ranged from 0.65 mg/g to 4.30 mg/g, flavonoids from 0.36 mg/g to 1.12 mg/g, and anthocyanins from 0.65 to 7.08 mg/g sample. 4-O-Caffeoyl-quinic acid was tentatively identified for the first time in Aronia. In order to characterize better chokeberry juice, a GC profile of aroma compounds was obtained. The aroma juice compounds belong mainly to the chemical classes of alcohols (48.9%) and ketones (30.28%). The most abundant compound is 3-penthen-2-one (23.6%).

Preparation of dimeric procyanidins B1, B2, B5, and B7 from a polymeric procyanidin fraction of black chokeberry ( Aronia melanocarpa ).

PubMed

Esatbeyoglu, Tuba; Winterhalter, Peter

2010-04-28

A semisynthetic approach has been used for the preparative formation of dimeric procyanidins B1, B2, B5, and B7. As starting material for the semisynthesis, polymeric procyanidins from black chokeberry were applied. These polymers were found to consist almost exclusively of (-)-epicatechin units. Under acidic conditions the interflavanoid linkages of the polymeric procyanidins are cleaved and the liberated (-)-epicatechin can react with nucleophiles, such as (+)-catechin or (-)-epicatechin. In this way, the polymeric procyanidins are degraded while dimeric procyanidins are formed. During this reaction only dimeric procyanidins are formed that contain (-)-epicatechin in the upper unit, that is, B1 [(-)-EC-4beta-->8-(+)-C)], B2 [(-)-EC-4beta-->8-(-)-EC], B5 [(-)-EC-4beta-->6-(-)-EC], and B7 [(-)-EC-4beta-->6-(+)-C]. The reaction mixtures of the semisynthesis can be successfully fractionated with high-speed countercurrent chromatography (HSCCC), and it is possible to isolate pure procyanidins B1, B2, B5, and B7 on a preparative scale.

Cytotoxicity of gemcitabine enhanced by polyphenolics from Aronia melanocarpa in pancreatic cancer cell line AsPC-1.

PubMed

Thani, Noor Azela Abdullah; Keshavarz, Sholeh; Lwaleed, Bashir A; Cooper, Alan J; Rooprai, Harcharan K

2014-11-01

Extending work with brain tumours, the hypothesis that micronutrients may usefully augment anticancer regimens, chokeberry (Aronia melanocarpa) extract was tested to establish whether it has pro-apoptotic effects in AsPC-1, an established human pancreatic cell line, and whether it potentiates cytotoxicity in combination with gemcitabine. Pancreatic cancer was chosen as a target, as its prognosis remains dismal despite advances in therapy. An MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay was used to assess the growth of the single pancreatic cancer cell line AsPC-1, alone and in comparison or combination with gemcitabine. This was backed up by flow cytometric DRAQ7 cell viability analysis. TUNEL assays were also carried out to investigate pro-apoptotic properties as responsible for the effects of chokeberry extract. Chokeberry extract alone and its IC75 value (1 µg/mL) in combination with gemcitabine were used to assess the growth of the AsPC-1 cell line. Gemcitabine in combination with chokeberry extract was more effective than gemcitabine alone. TUNEL assays showed apoptosis to be a mechanism occurring at 1 µg/mL concentration of chokeberry, with apoptotic bodies detected by both colourimetric and fluorometric methods. The implication of this study, using single cancer cell line, is that chemotherapy (at least with gemcitabine) might be usefully augmented with the use of micronutrients such as chokeberry extract. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Chokeberry (Aronia melanocarpa) - A review on the characteristic components and potential health effects.

PubMed

Kulling, Sabine E; Rawel, Harshadai M

2008-10-01

The intention of this review is to contribute to a better understanding of the potentials of the nutritional contribution of ARONIA berries ( ARONIA MELANOCARPA). The paper gives a short background to their botanical classification and cultivation practice, going in detail to describe the chemical composition of the berries. The emphasis is laid thereby upon the phenolic constituents. The paper finally gives a short resume of their beneficial effects in biological systems IN VITRO, in animals, and in humans, thus underlining their medicinal potential.

Extract of Aronia melanocarpa-modified hemostasis: in vitro studies.

PubMed

Sikora, Joanna; Markowicz-Piasecka, Magdalena; Broncel, Marlena; Mikiciuk-Olasik, Elżbieta

2014-10-01

Aronia melanocarpa has an extremely high content of procyanidins and anthocyanins. The multidirectional benefits of consumption of these berries are widely reported. Although numerous studies confirmed the influence of polyphenols on various stages of hemostasis, the exact mechanism of this phenomenon is not understood. The aim of our study was to evaluate the in vitro effect of A. melanocarpa extract on various parameters of hemostasis. Adenosine 5'-diphosphate (ADP)-induced aggregation was measured with turbidimetric method. Spontaneous and ADP-activated platelet adhesion were investigated using a colorimetric method. The global assay of coagulation and fibrinolysis was performed with the use of optical clotting and lysis (CL) test. Thrombin (0.5 IU/mL) and tissue plasminogen activator (60 ng/mL) were used to obtain a CL curve. The activity of thrombin and plasmin was determined by means of chromogenic substrate (S-2238, S-2251) RESULTS: The aronia extract contributed to the reduction in spontaneous and ADP-activated platelet adhesion. A significant increase in overall potential of CL as well as significant changes in key parameters of these processes (T t-thrombin time, F vo-initial plasma clotting velocity, and L max-maximum lysis) was reported. Chokeberry extract significantly inhibited the amidolytic activity of thrombin and plasmin. Our in vitro findings indicate a complex mechanism of influence of chokeberry polyphenols on platelet activity and the overall potential of CL. We confirmed that chokeberry inhibits the amidolytic activity of thrombin. It was demonstrated for the first time that chokeberry polyphenols inhibit the amidolytic activity of another serine protease, i.e., plasmin, which is the main fibrinolytic enzyme. Furthermore, our research points out a significant contribution of other plasma components and fibrinogen in the modulation of hemostasis by polyphenols.

Antioxidant Potential of Fruit Juice with Added Chokeberry Powder (Aronia melanocarpa).

PubMed

Šic Žlabur, Jana; Dobričević, Nadica; Pliestić, Stjepan; Galić, Ante; Bilić, Daniela Patricia; Voća, Sandra

2017-12-05

The purpose of this study was to determine the possibility of using chokeberry powder as a supplement in apple juice to increase the nutritional value of the final product with the aim of developing a new functional food product. Also, to determine the influence of ultrasound assisted extraction on the bioactive compounds content, nutritional composition and antioxidant potential of apple juice with added chokeberry powder. The juice samples with added chokeberry powder had higher antioxidant capacity, irrespective of the extraction technique used. Apple juice samples with added chokeberry powder treated with high intensity ultrasound had significantly higher content of all analyzed bioactive compounds. The application of high intensity ultrasound significantly reduced the extraction time of the plant material. A positive correlation between vitamin C content, total phenols, flavonoids and anthocyanins content and antioxidant capacity was determined in juice samples with added chokeberry powder treated with high intensity ultrasound.

Effect of Chokeberry (Aronia melanocarpa) juice on the metabolic activation and detoxication of carcinogenic N-nitrosodiethylamine in rat liver.

PubMed

Krajka-Kuźniak, Violetta; Szaefer, Hanna; Ignatowicz, Ewa; Adamska, Teresa; Oszmiański, Jan; Baer-Dubowska, Wanda

2009-06-10

Chokeberry is a rich source of polyphenols, which may counteract the action of chemical carcinogens. The aim of this study was to examine the effect of chokeberry juice alone or in combination with N-nitrosodiethylamine (NDEA) on phase I and phase II enzymes and DNA damage in rat liver. The forced feeding with chokeberry juice alone decreased the activities of enzymatic markers of cytochrome P450, CYP1A1 and 1A2. NDEA treatment also decreased the activity of CYP2E1 but enhanced the activity of CYP2B. Pretreatment with chokeberry juice further reduced the activity of these enzymes. Modulation of P450 enzyme activities was accompanied by the changes in the relevant proteins levels. Phase II enzymes were increased in all groups of animals tested. Chokeberry juice augmented DNA damage and aggravated the effect of NDEA. These results indicate that chokeberry may protect against liver damage; however, in combination with chemical carcinogens it might enhance their effect.

Induction of apoptosis and reduction of MMP gene expression in the U373 cell line by polyphenolics in Aronia melanocarpa and by curcumin.

PubMed

Abdullah Thani, Noor Azela; Sallis, Benjamin; Nuttall, Robert; Schubert, Frank R; Ahsan, Mansoor; Davies, Derek; Purewal, Sukhveer; Cooper, Alan; Rooprai, Harcharan K

2012-10-01

Malignant brain tumours are rare but are the most challenging types of cancers to treat. Despite conventional multimodality approaches available for their management, the outlook for most patients remains dismal due to the ability of the tumour cells to invade the normal brain. Attention has now focused on novel therapeutic interventions such as as the use of micronutrients. Both chokeberry extract (Aronia melanocarpa), which is rich in natural pigments such as anthocyanins and curcumin (diferuloylmethane) found in turmeric (Curcuma longa) have been reported to possess anticancer properties in other cancers. The aim of this study was to extend our previous research to evaluate the therapeutic potential of these two agents by testing their ability to induce apoptosis in an established glioblastoma cell line (U373). This was accomplished by treating the cells for 48 h with either chokeberry extract or curcumin, and using the Annexin-V assay. Gene profiles of 8 MMPs (2, 9, 14, 15, 16, 17, 24 and 25) and 4 TIMPs (1, 2, 3 and 4) were analysed for effects of mediators of invasion by quantitative real-time polymerase chain reaction (RT-PCR). The IC50 values determined for curcumin and chokeberry extract were 15 and 200 µg/ml, respectively. Our results also suggest that curcumin induces apoptosis but chokeberry extract is necrotic to this cell line. It is possible that chokeberry extract kills the cells by other non-apoptotic pathways. In addition, the RT-PCR results show downregulation of the gene expression of MMP-2, -14, -16 and -17 for both micronutrients. Taken together, the comparative data suggest that both curcumin and chokeberry extract may exhibit their anticancer potential by inducing apoptosis and inhibiting invasion by reducing MMP gene expression.

Phenolic Content, Antioxidant Capacity and Quality of Chokeberry (Aronia melanocarpa) Products.

PubMed

Tolić, Mandica-Tamara; Jurčević, Irena Landeka; Krbavčić, Ines Panjkota; Marković, Ksenija; Vahčić, Nada

2015-06-01

Chokeberries ( Aronia melanocarpa ) are rarely used in diet in Croatia but they have high content of polyphenolic compounds and one of the highest in vitro antioxidant activities among fruits. The aim of this study is to compare the quality, phenolic content and antioxidant capacity of different chokeberry products (juices, powders, fruit tea, capsules and dried berries). It can be expected that processing influences antioxidant activity and phenolic content of final products reaching consumers. Characterisation of phenolic compounds was carried out by using spectroscopic methods (Folin-Ciocalteu and pH differential methods). Antioxidant activity of chokeberry products was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The results show that the investigated products contain high amount of phenols (3002 to 6639 mg per L and 1494 to 5292 mg per 100 g of dry matter) and lower amount of total anthocyanins (150 to 1228 mg per L and 141 to 2468 mg per 100 g of dry matter). The examined juices and other chokeberry products possess high antioxidant capacity (12.09 to 40.19 mmol per L or 58.49 to 191.31 mmol per 100 g of dry matter, respectively) and reducing power (38.71 to 79.86 mmol per L or 13.50 to 68.60 mmol per 100 g of dry matter, respectively). On the basis of phenolic content and antioxidant activity, capsules and powders stand out among other products. The study indicates that there are significant differences (p<0.05) in the quality, phenolic content and antioxidant capacity among examined products.

Phenolic Content, Antioxidant Capacity and Quality of Chokeberry (Aronia melanocarpa) Products

PubMed Central

Jurčević, Irena Landeka; Krbavčić, Ines Panjkota; Marković, Ksenija; Vahčić, Nada

2015-01-01

Summary Chokeberries (Aronia melanocarpa) are rarely used in diet in Croatia but they have high content of polyphenolic compounds and one of the highest in vitro antioxidant activities among fruits. The aim of this study is to compare the quality, phenolic content and antioxidant capacity of different chokeberry products (juices, powders, fruit tea, capsules and dried berries). It can be expected that processing influences antioxidant activity and phenolic content of final products reaching consumers. Characterisation of phenolic compounds was carried out by using spectroscopic methods (Folin–Ciocalteu and pH differential methods). Antioxidant activity of chokeberry products was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The results show that the investigated products contain high amount of phenols (3002 to 6639 mg per L and 1494 to 5292 mg per 100 g of dry matter) and lower amount of total anthocyanins (150 to 1228 mg per L and 141 to 2468 mg per 100 g of dry matter). The examined juices and other chokeberry products possess high antioxidant capacity (12.09 to 40.19 mmol per L or 58.49 to 191.31 mmol per 100 g of dry matter, respectively) and reducing power (38.71 to 79.86 mmol per L or 13.50 to 68.60 mmol per 100 g of dry matter, respectively). On the basis of phenolic content and antioxidant activity, capsules and powders stand out among other products. The study indicates that there are significant differences (p<0.05) in the quality, phenolic content and antioxidant capacity among examined products. PMID:27904346

Distribution of apparent activation energy counterparts during thermo - And thermo-oxidative degradation of Aronia melanocarpa (black chokeberry).

PubMed

Janković, Bojan; Marinović-Cincović, Milena; Janković, Marija

2017-09-01

Kinetics of degradation for Aronia melanocarpa fresh fruits in argon and air atmospheres were investigated. The investigation was based on probability distributions of apparent activation energy of counterparts (ε a ). Isoconversional analysis results indicated that the degradation process in an inert atmosphere was governed by decomposition reactions of esterified compounds. Also, based on same kinetics approach, it was assumed that in an air atmosphere, the primary compound in degradation pathways could be anthocyanins, which undergo rapid chemical reactions. A new model of reactivity demonstrated that, under inert atmospheres, expectation values for ε a occured at levels of statistical probability. These values corresponded to decomposition processes in which polyphenolic compounds might be involved. ε a values obeyed laws of binomial distribution. It was established that, for thermo-oxidative degradation, Poisson distribution represented a very successful approximation for ε a values where there was additional mechanistic complexity and the binomial distribution was no longer valid. Copyright © 2017 Elsevier Ltd. All rights reserved.

Antidiabetic Effects of Aronia melanocarpa and Its Other Therapeutic Properties.

PubMed

Banjari, Ines; Misir, Andreja; Šavikin, Katarina; Jokić, Stela; Molnar, Maja; De Zoysa, H K S; Waisundara, Viduranga Y

2017-01-01

Diabetes is a global pandemic which warrants urgent attention due to its rising prevalence and economic burden. Thus, many alternative therapies are being researched for antidiabetic properties, given the inefficacy of current medicinal treatments. From this perspective, Aronia melanocarpa or black chokeberry has been investigated for its therapeutic properties in many studies, especially for its ability to combat hyperglycemia-induced oxidative stress and the macrovascular complications of diabetes including cardiovascular disease. Though A. melanocarpa is native to the eastern areas of North America, it has been planted extensively in Europe and Asia as well. Several in vivo studies have displayed the antioxidant properties of A. melanocarpa berry juice and plant extract in rat models where oxidative stress markers were observed to have significant reductions. Some of the potent bioactive compounds present in the fruits and other parts of the plant were identified as (-)-epicatechin, chlorogenic acid, neochlorogenic acid, and cyanidin-3-galactoside. Overall, A. melanocarpa could be considered a good source of antioxidants which is effective in combating hyperglycemia-induced oxidative stress.

Characterization of Aronia melanocarpa volatiles by headspace-solid-phase microextraction (HS-SPME), simultaneous distillation/extraction (SDE), and gas chromatography-olfactometry (GC-O) methods.

PubMed

Kraujalytė, Vilma; Leitner, Erich; Venskutonis, Petras Rimantas

2013-05-22

The profiles of volatile constituents of berry fruit of two Aronia melanocarpa genotypes were evaluated by headspace-solid-phase microextraction (HS-SPME), simultaneous distillation and extraction (SDE), and gas chromatography-olfactometry (GC-O). In total, 74 volatile compounds were identified in chokeberry juice, 3-penten-2-one, 3,9-epoxy-p-menth-1-ene, and benzaldehyde being the most abundant constituents; however, their percentage concentrations were remarkably different in the HS-SPME and SDE profiles. Twenty two aroma-active compounds were detected and characterized by the trained panelists in HS-SPME using GC-O detection frequency analysis. Olfactometry revealed that ethyl-2-methyl butanoate, ethyl-3-methyl butanoate, ethyl decanoate ("fruity" aroma notes), nonanal ("green" notes), unidentified compound possessing "moldy" odor, and some other volatiles may be very important constituents in formation of chokeberry aroma of both analyzed plant cultivars.

Anthocyans-rich Aronia melanocarpa extract possesses ability to protect endothelial progenitor cells against angiotensin II induced dysfunction.

PubMed

Parzonko, Andrzej; Oświt, Aleksandra; Bazylko, Agnieszka; Naruszewicz, Marek

2015-12-15

Endothelial progenitor cells (EPC) may provide protection against atherosclerosis and plaque rupture by their innate ability to replace dysfunctional or damaged endothelial cells in plaque microvessels. There is evidence that angiotensin II may impair the angiogenic functions of EPCs in the atherosclerotic plaque by accelerating senescence and inhibiting their proliferation through oxidative stress induction. In this study, we examined whether chokeberry (Aronia melanocarpa) fruit extract, containing mainly anthocyanins with potent antioxidative properties, could protect EPCs against angiotensin-induced oxidative stress. EPCs were isolated from peripheral blood of young healthy volunteers and cultivated on fibronectin-coated plates in the presence or absence of angiotensin II (1 µM) and chokeberry extract (1-25 µg/ml). EPCs exposed to chokeberry extract prior to angiotensin II showed a significant increase of proliferation and telomerase activity, and a decrease in the percentage of senescent cells and intracellular ROS formation in comparison to angiotensin II treated cells. Furthermore, extract increased migration ability, adhesion to fibronectin and the angiogenic potential of EPC in vitro diminished by angiotensin II in a concentration-dependent manner. That effect was related to the activation of the Nrf2 transcription factor and the increase of HO-1 expression. Our results suggested that chokeberry extract may protect EPCs against angiotensin II-induced dysfunction and could play a potential role in the prevention of coronary artery disease. Copyright © 2015 Elsevier GmbH. All rights reserved.

Aronia melanocarpa (Black Chokeberry) Reduces Ethanol-Induced Gastric Damage via Regulation of HSP-70, NF-κB, and MCP-1 Signaling.

PubMed

Paulrayer, Antonisamy; Adithan, Aravinthan; Lee, Jeong Ho; Moon, Kwang Hyun; Kim, Dae Geun; Im, So Yeon; Kang, Chang-Won; Kim, Nam Soo; Kim, Jong-Hoon

2017-06-05

Aronia melanocarpa (Michx.) Ell. belongs to the Rosaceae family. The purpose of this study is to explore the gastroprotective effect of the Aronia melanocarpa hydro-alcoholic extract (AMHAE) against ethanol-induced gastric ulcer in a rat model. Different concentrations (50, 100, and 200 mg/kg) of AMHAE, or 30 mg/kg of omeprazole, significantly inhibited the gastric injury formation. The ethanol-induced ulcer group showed significant increases of malondialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor (TNF)-α, nuclear factor-kappaB p65 (NF-κB p65), and monocyte chemoattractant protein (MCP)-1, and decreased activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-px), and interleukin (IL)-4. However, AMHAE (200 mg/kg) pretreatment significantly reversed the altered pathophysiological levels of these biomolecules to near normal stages. The gastroprotective activity of AMHAE was abolished by pretreatment with l-NAME, naloxone, capsazepine, and indomethacin, demonstrating the participation of nitric oxide (NO), opioids, TRPV (vanilloid receptor-related transient receptor potential), and prostaglandins in AMHAE-assisted gastroprotection against ethanol-induced gastric injuries. This gastroprotective effect of AMHAE might be due to the downregulation of TNF-α-based NF-κB, MCP-1 signaling and strong antioxidant properties.

Aronia melanocarpa (Black Chokeberry) Reduces Ethanol-Induced Gastric Damage via Regulation of HSP-70, NF-κB, and MCP-1 Signaling

PubMed Central

Paulrayer, Antonisamy; Adithan, Aravinthan; Lee, Jeong Ho; Moon, Kwang Hyun; Kim, Dae Geun; Im, So Yeon; Kang, Chang-Won; Kim, Nam Soo; Kim, Jong-Hoon

2017-01-01

Aronia melanocarpa (Michx.) Ell. belongs to the Rosaceae family. The purpose of this study is to explore the gastroprotective effect of the Aronia melanocarpa hydro-alcoholic extract (AMHAE) against ethanol-induced gastric ulcer in a rat model. Different concentrations (50, 100, and 200 mg/kg) of AMHAE, or 30 mg/kg of omeprazole, significantly inhibited the gastric injury formation. The ethanol-induced ulcer group showed significant increases of malondialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor (TNF)-α, nuclear factor-kappaB p65 (NF-κB p65), and monocyte chemoattractant protein (MCP)-1, and decreased activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-px), and interleukin (IL)-4. However, AMHAE (200 mg/kg) pretreatment significantly reversed the altered pathophysiological levels of these biomolecules to near normal stages. The gastroprotective activity of AMHAE was abolished by pretreatment with l-NAME, naloxone, capsazepine, and indomethacin, demonstrating the participation of nitric oxide (NO), opioids, TRPV (vanilloid receptor-related transient receptor potential), and prostaglandins in AMHAE-assisted gastroprotection against ethanol-induced gastric injuries. This gastroprotective effect of AMHAE might be due to the downregulation of TNF-α-based NF-κB, MCP-1 signaling and strong antioxidant properties. PMID:28587230

Chokeberry (Aronia melanocarpa) juice modulates 7,12-dimethylbenz[a]anthracene induced hepatic but not mammary gland phase I and II enzymes in female rats.

PubMed

Szaefer, Hanna; Krajka-Kuźniak, Violetta; Ignatowicz, Ewa; Adamska, Teresa; Baer-Dubowska, Wanda

2011-03-01

Chokeberry is a rich source of procyanidins known to have several types of biological activity including anticarcinogenic potential in experimental models. In this study we examined the effect of chokeberry juice on the hepatic and mammary gland carcinogen metabolizing enzyme expression altered by the polycyclic aromatic hydrocarbon, 7,12-dimethylbenz[a]anthracene (DMBA). Sprague-Dawley rats were gavaged with chokeberry juice (8 ml/kg b.w.) for 28 consecutive days. DMBA was administered i.p. on the 27th and the 28th days. Pretreatment with chokeberry juice reduced the activity of CYP1A1 and increased that of CYP2B involved in metabolic activation/detoxication of DMBA in rat liver, as well as expression and activity of phase II enzymes. Chokeberry juice had no effect on these parameters in the mammary gland and DMBA induced DNA damage in rat blood cells. These results together with our earlier observations indicate that metabolic alterations induced by chokeberry feeding are tissue specific and depend on the class of carcinogen. Copyright © 2011 Elsevier B.V. All rights reserved.

The influence of interactions among phenolic compounds on the antiradical activity of chokeberries (Aronia melanocarpa).

PubMed

Jakobek, Lidija; Seruga, Marijan; Krivak, Petra

2011-06-01

In the present work, interactions between phenolic compounds from chokeberries and their influence on the antiradical activity was studied. Three fractions were isolated from chokeberries containing different classes of phenolic compounds. The first fraction contained a major part of phenolic acids and flavonols, the second anthocyanins, and the third insoluble phenols and proanthocyanidins. The phenolic compound content was determined using high-performance liquid chromatography, and the antiradical activity using the DPPH test. In order to evaluate the effects of interactions between phenolic compounds on the antiradical activity, the antiradical activity of individual phenolic fractions was compared with that obtained by mixing phenolic fractions. Phenolic mixtures showed the decrease in the antiradical activity in comparison with the individual phenolic fractions. These results suggest the existence of complex interactions among phenolic compounds that caused the decrease of the antiradical activity. Interactions among chokeberry phenols promoted a negative synergism.

COMMERCIAL EXTRACT FROM ARONIA AS A MODULATOR OF ADHESIVE PROPERTIES OF FIBRINOGEN TREATED WITH HOMOCYSTEINE AND ITS THIOLACTONE IN VITRO.

PubMed

Olas, Beata; Kontek, Bogdan; Oleszek, Wieslaw; Stochmal, Anna

2016-11-01

Research has confirmed the positive effect of berries of Aivnia melanocarpa on the cardiovascular system. The protective effects of polyphenol-rich extract from berries of A. melanocarpa against changes in biological properties of fibrinogen were studied. In in vino model of hyperhomocysteinemia the capability of fibrinogen to interact with human blood platelets was measured by platelet adhesion in the presence of extract fromA. nelanocapa. We induced hyperhomocystenemia using a reduced form of homocysteine (Hey, at a final concentration of 0.01. 0.1 and 1 μM) and the most reactive form of Hey - its cyclic thioester, homocysteine thiolactone (HTL, at a final concentration of 0.1, 0.5 and I μM). It was observed that Hey or HTL-treated fibrinogen, in comparison with untreated molecule, had a distinct capability to mediate blood platelet adhesion. The experiments also indicate that polyphenol-rich extract from black chokeberries (at final concentrations of 2.5-10 pM/mL) reduced the toxic action of Hey and HTL on the adhesive properties of fibrinogen. The possible protection exerted by black chokeberry extract, through restoring the platelet adhesion of Hey or HTL treated fibrinogen, may be important for vascular diseases.

Antidiabetic Effects of Aronia melanocarpa and Its Other Therapeutic Properties

PubMed Central

Banjari, Ines; Misir, Andreja; Šavikin, Katarina; Jokić, Stela; Molnar, Maja; De Zoysa, H. K. S.; Waisundara, Viduranga Y.

2017-01-01

Diabetes is a global pandemic which warrants urgent attention due to its rising prevalence and economic burden. Thus, many alternative therapies are being researched for antidiabetic properties, given the inefficacy of current medicinal treatments. From this perspective, Aronia melanocarpa or black chokeberry has been investigated for its therapeutic properties in many studies, especially for its ability to combat hyperglycemia-induced oxidative stress and the macrovascular complications of diabetes including cardiovascular disease. Though A. melanocarpa is native to the eastern areas of North America, it has been planted extensively in Europe and Asia as well. Several in vivo studies have displayed the antioxidant properties of A. melanocarpa berry juice and plant extract in rat models where oxidative stress markers were observed to have significant reductions. Some of the potent bioactive compounds present in the fruits and other parts of the plant were identified as (−)-epicatechin, chlorogenic acid, neochlorogenic acid, and cyanidin-3-galactoside. Overall, A. melanocarpa could be considered a good source of antioxidants which is effective in combating hyperglycemia-induced oxidative stress. PMID:29164127

Bioavailability of cyanidin glycosides from natural chokeberry (Aronia melanocarpa) juice with dietary-relevant dose of anthocyanins in humans.

PubMed

Wiczkowski, Wieslaw; Romaszko, Ewa; Piskula, Mariusz K

2010-12-08

The aim of this study was to investigate the bioavailability of anthocyanins from chokeberry juice with a dietary-relevant dose of anthocyanins. Thirteen healthy volunteers consumed chokeberry juice providing 0.8 mg of anthocyanins/kg of body weight. Before and after juice consumption, blood and urine were collected. Concentration of anthocyanins was measured with HPLC-PDA-MS-ESI. Cyanidin-3-galactoside comprised 66% of total chokeberry anthocyanins. Eight cyanidin derivatives were found in blood and urine after juice consumption. The maximum plasma anthocyanin concentration of 32.7 ± 2.9 nmol/L was reached at 1.3 ± 0.1 h after juice consumption. The anthocyanins' urine excretion rate (62.9 ± 5.0 nmol/h) was the highest within the first 2 h. In total, 0.25 ± 0.02% of the ingested anthocyanins was excreted by the renal route during 24 h, mainly as metabolites of cyanidin. According to these observations, after consumption of a dietary-relevant dose of anthocyanins as natural chokeberry juice, anthocyanins and their metabolites were present in plasma and urine of volunteers.

Anthocyanin-rich Aronia melanocarpa extract improves body temperature maintenance in healthy women with a cold constitution.

PubMed

Sonoda, Keisuke; Aoi, Wataru; Iwata, Tomoaki; Li, Yanmei

2013-01-01

Specific anthocyanin-rich dietary factors have been shown to improve metabolic functions associated with thermogenesis in animal studies. Aronia melanocarpa, commonly known as wild chokeberry, contains a high level of anthocyanin that would be expected to maintain body temperature through thermogenesis. We here investigated the effects of Aronia melanocarpa extracts on body temperature and peripheral blood flow in healthy women with a cold constitution. A pre/post comparison trial was performed in 11 women with a cold constitution, who were taking Aronia melanocarpa extracts (150 mg/day) for 4 weeks. Physiological and biochemical parameters, along with psychological tests were examined. The subjects' body surface temperature was significantly higher in the post-trial than in the pre-trial. In psychological tests, factors related to cold were significantly improved by Aronia intake. On the other hand, peripheral blood flow was not affected by Aronia supplementation. Plasma noradrenalin level was significantly elevated by Aronia intake, and subjects with a higher level of 8-hydroxy-2'-deoxyguanosine in the pre-trial showed decreased levels in the post-trial. These data suggest that dietary Aronia melanocarpa extract improves the maintenance of body temperature in healthy women with a cold constitution, which may be mediated by noradrenalin and oxidative stress levels.

Cardioprotective effects of Aronia melanocarpa anthocynanins. From laboratory experiments to clinical practice.

PubMed

Parzonko, Andrzej; Naruszewicz, Marek

2016-01-01

The role of polyphenols in the cardiovascular diseases prevention is still a matter of scientific discussion. However, recent clinical studies indicate that intake of anthocyanins and in a lesser extent procyanidins can participate in prevention of hypertension and type 2 diabetes. Fruits of Aronia melanocarpa (chokeberry) are known to be a reach source of these polyphenols. Moreover, its extracts were shown to express strong antioxidant, antiinflammatory, vasorelaxant and antithrombotic properties. The aim of the review is to summarize the results of the hitherto research regarding the biological effects at the molecular and clinical level.

Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cells

PubMed Central

Rugină, Dumitriţa; Sconţa, Zoriţa; Leopold, Loredana; Pintea, Adela; Bunea, Andrea

2012-01-01

Abstract The present study evaluates the antioxidant activity of two Aronia melanocarpa cultivars—Viking and Aron—and of Aronia prunifolia hybrid in relationship with their phytochemical composition regarding the contents of total phenolics, flavonoids, procyanidins, and monomeric anthocyanins. The antioxidant capacity of the mentioned extracts of chokeberries was evaluated through five complementary assays: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), H2O2 scavenging potential, oxygen radical absorbance capacity, ferric reducing antioxidant power, and cupric ion reducing antioxidant capacity. A. prunifolia hybrid was found to have the highest antioxidant activity and to be the richest in polyphenols, procyanidins, and anthocyanins compared with the A. melanocarpa cultivars. A good correlation was observed between antioxidant activity and total procyanidin and anthocyanin content. Cyanidin glycosides inhibited HeLa human cervical tumor cell proliferation and increased generation of reactive oxygen species after 48 h of treatment, suggesting that they could be responsible for the antiproliferative activity. These results may be significant for industry concerning food quality and disease prevention. PMID:22846076

Aronia melanocarpa Elliot reduces the activity of angiotensin i-converting enzyme-in vitro and ex vivo studies.

PubMed

Sikora, Joanna; Broncel, Marlena; Mikiciuk-Olasik, Elżbieta

2014-01-01

The aim of the study was to analyze the effects of two-month supplementation with chokeberry preparation on the activity of angiotensin I-converting enzyme (ACE) in patients with metabolic syndrome (MS). During the in vitro stage of the study, we determined the concentration of chokeberry extract, which inhibited the activity of ACE by 50% (IC50). The participants (n = 70) were divided into three groups: I-patients with MS who received chokeberry extract supplements, II-healthy controls, and III-patients with MS treated with ACE inhibitors. After one and two months of the experiment, a decrease in ACE activity corresponded to 25% and 30%, respectively. We documented significant positive correlations between the ACE activity and the systolic (r = 0.459, P = 0.048) and diastolic blood pressure, (r = 0.603, P = 0.005) and CRP. The IC50 of chokeberry extract and captopril amounted to 155.4 ± 12.1 μg/mL and 0.52 ± 0.18 μg/mL, respectively. Our in vitro study revealed that chokeberry extract is a relatively weak ACE inhibitor. However, the results of clinical observations suggest that the favorable hypotensive action of chokeberry polyphenols may be an outcome of both ACE inhibition and other pleotropic effects, for example, antioxidative effect.

Effect of UV-C Radiation, Ultra-Sonication Electromagnetic Field and Microwaves on Changes in Polyphenolic Compounds in Chokeberry (Aronia melanocarpa).

PubMed

Cebulak, Tomasz; Oszmiański, Jan; Kapusta, Ireneusz; Lachowicz, Sabina

2017-07-12

Chokeberry fruits are highly valued for their high content of polyphenolic compounds. The use of such abiotic stress factors as UV-C radiation, an electromagnetic field, microwave radiation, and ultrasound, at different operation times, caused differentiation in the contents of anthocyanins, phenolic acids, flavonols, and flavan-3-ols. Samples were analyzed for contents of polyphenolics with ultra-performance liquid chromatography and photodiode detector-quadrupole/time-of-flight mass spectrometry (UPLC-PDA-MS/MS). The analysis showed that after exposure to abiotic stress factors, the concentration of anthocyanins ranged from 3587 to 6316 mg/100 g dry matter (dm) that constituted, on average, 67.6% of all identified polyphenolic compounds. The second investigated group included phenolic acids with the contents ranging between 1480 and 2444 mg/100 g dm (26.5%); then flavonols within the range of 133 to 243 mg/100 g dm (3.7%), and finally flavan-3-ols fluctuated between 191 and 369 mg/100 g dm (2.2%). The use of abiotic stress factors such as UV-C radiation, microwaves and ultrasound field, in most cases contributed to an increase in the content of the particular polyphenolic compounds in black chokeberry. Under the influence of these factors, increases were observed: in anthocyanin content, of 22%; in phenolic acids, of 20%; in flavonols, of 43%; and in flavan-3-ols, of 30%. Only the use of the electromagnetic field caused a decrease in the content of the examined polyphenolic compounds.

Effect of the Production of Dried Fruits and Juice from Chokeberry (Aronia melanocarpa L.) on the Content and Antioxidative Activity of Bioactive Compounds

DOE PAGES

Oszmiański, Jan; Lachowicz, Sabina

2016-08-22

The aim of this study was to evaluate the production of dried fruits and juices from chokeberry as potential sources of bioactive compounds with beneficial effects on human health. Dry powders and juices from chokeberry were analyzed for the contents of sugars with high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD method), and the antioxidant capacity was analyzed by the FRAP (ferric-reducing ability of plasma) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) tests. Polyphenols were then identified by high performance liquid chromatography (HPLC) coupled with a tandem mass spectrometer and a photodiode-array detector (LC-PDA-ESI-MS/MS), and their quantitative analysis was carriedmore » out by UPLC-MS/MS (using a Q/TOF detector and a PDA detector). A total of 27 polyphenolic compounds was identified in chokeberry products, including 7 anthocyanins, 11 flavonols, 5 phenolic acids, 3 flavan-3-ols and 1 flavanone. Three anthocyanin derivatives were reported for the first time from chokeberry fruit. A higher activity of the bioactive compounds was determined in dried fruit pomace and in juice obtained from crushed fruits than in those from the whole fruits. In addition, the pomace was found to be a better material for the production of dry powders, compared to chokeberry fruits.« less

Effect of the Production of Dried Fruits and Juice from Chokeberry (Aronia melanocarpa L.) on the Content and Antioxidative Activity of Bioactive Compounds

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oszmiański, Jan; Lachowicz, Sabina

The aim of this study was to evaluate the production of dried fruits and juices from chokeberry as potential sources of bioactive compounds with beneficial effects on human health. Dry powders and juices from chokeberry were analyzed for the contents of sugars with high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD method), and the antioxidant capacity was analyzed by the FRAP (ferric-reducing ability of plasma) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) tests. Polyphenols were then identified by high performance liquid chromatography (HPLC) coupled with a tandem mass spectrometer and a photodiode-array detector (LC-PDA-ESI-MS/MS), and their quantitative analysis was carriedmore » out by UPLC-MS/MS (using a Q/TOF detector and a PDA detector). A total of 27 polyphenolic compounds was identified in chokeberry products, including 7 anthocyanins, 11 flavonols, 5 phenolic acids, 3 flavan-3-ols and 1 flavanone. Three anthocyanin derivatives were reported for the first time from chokeberry fruit. A higher activity of the bioactive compounds was determined in dried fruit pomace and in juice obtained from crushed fruits than in those from the whole fruits. In addition, the pomace was found to be a better material for the production of dry powders, compared to chokeberry fruits.« less

Aronia melanocarpa Elliot Reduces the Activity of Angiotensin I-Converting Enzyme—In Vitro and Ex Vivo Studies

PubMed Central

Sikora, Joanna; Broncel, Marlena; Mikiciuk-Olasik, Elżbieta

2014-01-01

Purpose. The aim of the study was to analyze the effects of two-month supplementation with chokeberry preparation on the activity of angiotensin I-converting enzyme (ACE) in patients with metabolic syndrome (MS). During the in vitro stage of the study, we determined the concentration of chokeberry extract, which inhibited the activity of ACE by 50% (IC50). Methods. The participants (n = 70) were divided into three groups: I—patients with MS who received chokeberry extract supplements, II—healthy controls, and III—patients with MS treated with ACE inhibitors. Results. After one and two months of the experiment, a decrease in ACE activity corresponded to 25% and 30%, respectively. We documented significant positive correlations between the ACE activity and the systolic (r = 0.459, P = 0.048) and diastolic blood pressure, (r = 0.603, P = 0.005) and CRP. The IC50 of chokeberry extract and captopril amounted to 155.4 ± 12.1 μg/mL and 0.52 ± 0.18 μg/mL, respectively. Conclusions. Our in vitro study revealed that chokeberry extract is a relatively weak ACE inhibitor. However, the results of clinical observations suggest that the favorable hypotensive action of chokeberry polyphenols may be an outcome of both ACE inhibition and other pleotropic effects, for example, antioxidative effect. PMID:25050143

Effect of the Production of Dried Fruits and Juice from Chokeberry (Aronia melanocarpa L.) on the Content and Antioxidative Activity of Bioactive Compounds.

PubMed

Oszmiański, Jan; Lachowicz, Sabina

2016-08-22

The aim of this study was to evaluate the production of dried fruits and juices from chokeberry as potential sources of bioactive compounds with beneficial effects on human health. Dry powders and juices from chokeberry were analyzed for the contents of sugars with high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD method), and the antioxidant capacity was analyzed by the FRAP (ferric-reducing ability of plasma) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) tests. Polyphenols were identified by high performance liquid chromatography (HPLC) coupled with a tandem mass spectrometer and a photodiode-array detector (LC-PDA-ESI-MS/MS), and their quantitative analysis was carried out by UPLC-MS/MS (using a Q/TOF detector and a PDA detector). A total of 27 polyphenolic compounds was identified in chokeberry products, including 7 anthocyanins, 11 flavonols, 5 phenolic acids, 3 flavan-3-ols and 1 flavanone. Three anthocyanin derivatives were reported for the first time from chokeberry fruit. A higher activity of the bioactive compounds was determined in dried fruit pomace and in juice obtained from crushed fruits than in those from the whole fruits. In addition, the pomace was found to be a better material for the production of dry powders, compared to chokeberry fruits.

Anthocyanin-rich Phytochemicals from Aronia Fruits Inhibit Visceral Fat Accumulation and Hyperglycemia in High-fat Diet-induced Dietary Obese Rats.

PubMed

Takahashi, Azusa; Shimizu, Hisae; Okazaki, Yukako; Sakaguchi, Hirohide; Taira, Toshio; Suzuki, Takashi; Chiji, Hideyuki

2015-01-01

Aronia fruits (chokeberry: Aronia melanocarpa E.) containing phenolic phytochemicals, such as cyanidin 3-glycosides and chlorogenic acid, have attracted considerable attention because of their potential human health benefits in humans including antioxidant activities and ability to improved vision. In the present study, the effects of anthocyanin-rich phytochemicals from aronia fruits (aronia phytochemicals) on visceral fat accumulation and fasting hyperglycemia were examined in rats fed a high-fat diet (Experiment 1). Total visceral fat mass was significantly lower in rats fed aronia phytochemicals than that in both the control group and bilberry phytochemicals-supplemented rats (p < 0.05). Moreover, perirenal and epididymal adipose tissue mass in rats fed aronia phytochemicals was significantly lower than that in both the control and bilberry phytochemicals group. Additionally, the mesenteric adipose tissue mass in aronia phytochemicals-fed rats was significantly low (p < 0.05). Furthermore, the fasting blood glucose levels significantly decreased in rats fed aronia phytochemicals for 4 weeks compared to that in the control rats (p < 0.05). Therefore, we investigated the effects of phytochemicals on postprandial hyperlipidemia after corn oil loading in rats, pancreatic lipase activity in vitro, and the plasma glycemic response after sucrose loading in order to elucidate the preventive factor of aronia phytochemical on visceral fat accumulation. In the oral corn oil tolerance tests (Experiment 2), aronia phytochemicals significantly inhibited the increases in plasma triglyceride levels, with a half-maximal inhibitory concentration (IC(50)) of 1.50 mg/mL. However, the inhibitory activity was similar to that of bilberry and tea catechins. In the sucrose tolerance tests (Experiment 3), aronia phytochemicals also significantly inhibited the increases in blood glucose levels that were observed in the control animals (p < 0.05). These results suggest that anthocyanin-rich phytochemicals in aronia fruits suppress visceral fat accumulation and hyperglycemia by inhibiting pancreatic lipase activity and/or intestinal lipid absorption.

Radical-scavenging-linked antioxidant activities of extracts from black chokeberry and blueberry cultivated in Korea.

PubMed

Hwang, Seok Joon; Yoon, Won Byong; Lee, Ok-Hwan; Cha, Seung Ju; Kim, Jong Dai

2014-03-01

The objective of this study was to investigate the radical-scavenging-linked antioxidant properties of the extracts from black chokeberry and blueberry cultivated in Korea. The 70% ethanol extracts were prepared from black chokeberry and blueberry, and evaluated for total phenolic content, total flavonoid content, total proanthocyanidin content, and antioxidative activities, using various in vitro assays, such as DPPH(2,2-diphenyl-1-picrylhydrazyl), ABTS(2,2-azino-bis-(3-ethylenebenzothiozoline-6-sulphonic acid)) radical-scavenging activity, FRAP(ferric-reducing antioxidant power) and reducing power. The major phenolic compounds, including cyanidin-3-galactoside, cyanidin-3-arabinoside, neochlorogenic acid, procyanidin B1, were analysed by HPLC with a photodiode array detector. Results showed that total phenol, flavonoid and proanthocyanidin contents of black chokeberry extract were higher than those of blueberry extract. In addition, black chokeberry extract exhibited higher free radical-scavenging activity and reducing power than did blueberry extract. Cyanidin-3-galactoside was identified as a major phenolic compound, with considerable content in black chokeberry, that correlated with its higher antioxidant and radical-scavenging effects. These results suggest that black chokeberry extracts could be considered as a good source of natural antioxidants and functional food ingredients. Copyright © 2013 Elsevier Ltd. All rights reserved.

Composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species.

PubMed

Mikulic-Petkovsek, Maja; Schmitzer, Valentina; Slatnar, Ana; Stampar, Franci; Veberic, Robert

2012-10-01

Sugars, organic acids, and total phenolic content in fruit of 25 wild and cultivated berry species were identified and quantified with high-performance liquid chromatograph. The composition of sugars, organic acids, and total phenolic compounds in various species of Vaccinium, Rubus, Ribes, and Fragaria genus was evaluated. Additonally, total phenolics of less known berry species of the Morus, Amelanchier, Sorbus, Sambucus, Rosa, Lycium, Actinidia, and Aronia genus were determined in wild growing as well as in cultivated fruits. Significant differences in the concentration of sugars and organic acids were detected among the berry species. Glucose and fructose were the most abundant sugars in berry fruits and the major organic acids were malic and citric acid. However, in kiwi fruit, sucrose represented as much as 71.9% of total sugars. Sorbitol has been detected and quantified in chokeberry, rowanberry, and eastern shadbush fruit. The highest content of total analyzed sugars was determined in rowanberry fruit, followed by dog rose, eastern shadbush, hardy kiwifruit, American cranberry, chokeberry, and jostaberry fruit. Rowanberry stands out as the fruit with the highest content of total analyzed organic acids, followed by jostaberry, lingonberry, red gooseberry, hardy kiwifruit, and black currant. The berries of white gooseberry, black currant, red currant, and white currant had the lowest sugar/organic acid ratio and were thus perceptively the sourest species analyzed. On the other hand, the species with highest sugar/organic acid ratio were goji berry, eastern shadbush, black mulberry, and wild grown blackberry. The highest amounts of total phenols were quantified in chokeberry fruit. Wild strawberry, raspberry, and blackberry had 2- to 5-fold more total phenolics compared to cultivated plants. The fruit of analyzed berry species contained different levels of sugars, organic acids, and total phenolics. Moreover, it has been demonstrated that wild grown species generally contain more phenolics than cultivated ones. This information is interesting for nutritionists as well as berry growers and breeders who can promote the cultivation of species and new cultivars with higher phenolic content. © 2012 Institute of Food Technologists®

Unraveling Metabolic Variation for Blueberry and Chokeberry Cultivars Harvested from Different Geo-Climatic Regions in Korea.

PubMed

Sim, Inseon; Suh, Dong Ho; Singh, Digar; Do, Seon-Gil; Moon, Kwang Hyun; Lee, Jeong Ho; Ku, Kang-Mo; Lee, Choong Hwan

2017-10-18

Temporal geo-climatic variations are presumably vital determinants of phenotypic traits and quality characteristics of berries manifested through reconfigured metabolomes. We performed an untargeted mass spectrometry (MS)-based metabolomic analysis of blueberry (Vaccinium spp.) and chokeberry (Aronia melanocarpa) sample extracts harvested from different geo-climatic regions in Korea. The multivariate statistical analysis indicated distinct metabolite compositions of berry groups based on different species and regions. The amino acids levels were relatively more abundant in chokeberry than in blueberry, while the sugar contents were comparatively higher in blueberry. However, the metabolite compositions were also dependent on geo-climatic conditions, especially latitude. Notwithstanding the cultivar types, amino acids, and sucrose were relatively more abundant in berries harvested from 35°N and 36°N geo-climatic regions, respectively, characterized by distinct duration of sunshine and rainfall patterns. The present study showed the ability of a metabolomics approach for recapitulating the significance of geo-climatic parameters for quality characterization of commercial berry types.

Effect of an Extract from Aronia melanocarpa L. Berries on the Body Status of Zinc and Copper under Chronic Exposure to Cadmium: An In Vivo Experimental Study.

PubMed

Borowska, Sylwia; Brzóska, Małgorzata M; Gałażyn-Sidorczuk, Małgorzata; Rogalska, Joanna

2017-12-19

In an experimental model of low-level and moderate environmental human exposure to cadmium (Cd), it was investigated whether the consumption of a polyphenol-rich Aronia melanocarpa L. berries (chokeberries) extract (AE) may influence the body status of zinc (Zn) and copper (Cu). The bioelements' apparent absorption, body retention, serum and tissue concentrations, total pool in internal organs, excretion, and the degree of binding to metallothionein were evaluated in female rats administered 0.1% aqueous AE or/and Cd in their diet (1 and 5 mg/kg) for 3-24 months. The consumption of AE alone had no influence on the body status of Zn and Cu. The extract administration at both levels of Cd treatment significantly (completely or partially) protected against most of the changes in the metabolism of Zn and Cu caused by this xenobiotic; however, it increased or decreased some of the Cd-unchanged indices of their body status. Based on the findings, it seems that rational amounts of chokeberry products may be included in the daily diet without the risk of destroying Zn and Cu metabolisms; however, their potential prophylactic use under exposure to Cd needs further study to exclude any unfavourable impact of these essential elements on the metabolism.

Reduction of anxiety-like and depression-like behaviors in rats after one month of drinking Aronia melanocarpa berry juice.

PubMed

Tomić, Mirko; Ignjatović, Đurđica; Tovilović-Kovačević, Gordana; Krstić-Milošević, Dijana; Ranković, Slavica; Popović, Tamara; Glibetić, Marija

2016-07-13

The treatment of mood and anxiety disorders by nutraceuticals is gaining growing awareness. Berries of Aronia melanocarpa (Black chokeberry) and their extracts, exceptionally abundant in diverse phenolic compounds, have become famous for the highest in vitro antioxidant activity among fruits and notable health benefits (e.g. anti-diabetic, anti-inflammatory, cardioprotective). This study was designed to investigate the behavioral effects of month-long unlimited consumption of Aronia master juice (AJ) and/or juice reconstruct without polyphenols (RJ), in young male rats. AJ was initially evaluated for its content of phenolic compounds by spectrophotometric assays and HPLC-DAD. Rats that were supplied with three various water concentrations of AJ and RJ, respectively: 20% + 0% (ARO group), 5% + 15% (RAJ) and 0 + 20% (PLC), were compared with those which consumed only water (CTL). Daily drinking of AJ solution was significantly elevated from the second or third week onward, which was most expressed in the ARO group. Only this group displayed behavioral variations, manifested by certain hyperactivity in open field tests and prominent reductions of anxiety-like behaviors in the elevated plus maze. The ARO rats also expressed an alleviation of depression-like behavior in forced swimming tests. These findings demonstrate the beneficial behavioral effects of the one-month-long free drinking of phenolic-rich AJ in rats (>20 ml per kg b. mass daily) that may be recognized as stimulating, anxiolytic-like and antidepressant-like. The in vitro assays suggested that MAO-A/MAO-B inhibitions by the phenolic compounds of AJ might be the possible in vivo mechanisms for such behavioral actions.

In vitro study of biological activities of anthocyanin-rich berry extracts on porcine intestinal epithelial cells.

PubMed

Kšonžeková, Petra; Mariychuk, Ruslan; Eliašová, Adriana; Mudroňová, Dagmar; Csank, Tomáš; Király, Ján; Marcinčáková, Dana; Pistl, Juraj; Tkáčiková, L'udmila

2016-03-15

Anthocyanins, compounds that represent the major group of flavonoids in berries, are one of the most powerful natural antioxidants. The aim of this study was to evaluate biological activities and comparison of anthocyanin-rich extracts prepared from chokeberry (Aronia melanocarpa), elderberry (Sambucus nigra), bilberry (Vaccinium myrtillus) and blueberry (V. corymbosum) on the porcine intestinal epithelial IPEC-1 cell line. The IC50 values calculated in the antioxidant cell-based dichlorofluorescein assay (DCF assay) were 1.129 mg L(-1) for chokeberry, 1.081 mg L(-1) for elderberry, 2.561 mg L(-1) for bilberry and 2.965 mg L(-1) for blueberry, respectively. We found a significant negative correlation (P < 0.001) between cyanidin glycosides content and IC50 values. Moreover, extracts rich in cyanidin glycosides stimulated proliferation of IPEC-1 cells and did not have cytotoxic effect on cells at an equivalent in vivo concentration. We found that the chokeberry and elderberry extracts rich in cyanidin glycosides possess better antioxidant and anticytotoxic activities in comparison to blueberry or bilberry extracts with complex anthocyanin profiles. © 2015 Society of Chemical Industry.

Protective effect of Aronia melanocarpa polyphenols against cadmium-induced disorders in bone metabolism: a study in a rat model of lifetime human exposure to this heavy metal.

PubMed

Brzóska, Malgorzata M; Rogalska, Joanna; Galazyn-Sidorczuk, Malgorzata; Jurczuk, Maria; Roszczenko, Alicja; Tomczyk, Michal

2015-03-05

It was investigated, in a female rat model of low and moderate lifetime human exposure to cadmium (Cd), whether polyphenols from Aronia melanocarpa berries (chokeberry; AMP) may offer protection from this heavy metal-induced disorders in bone metabolism. For this purpose, numerous indices of bone formation (osteocalcin, alkaline phosphatase, osteoprotegerin) and resorption (carboxy-terminal cross-linking telopeptides of type I collagen, soluble receptor activator of nuclear factor-κB ligand) in the serum and/or distal femur epiphysis (trabecular bone region), as well as bone mineral status (volumetric bone mineral density of the femur and content of mineral components, including calcium, in the bone tissue at the distal femur epiphysis) were evaluated in female Wistar rats that received a 0.1% aqueous extract of AMP, as the only drinking fluid (prepared from lyophilized extract by Adamed Consumer Healthcare), and/or Cd in diet (1 and 5mg/kg) for 3, 10, 17, and 24 months. Examination of the phytochemical profile of the aronia extract revealed high content of polyphenols (612.40 ± 3.33 mg/g), including anthocyanins, proanthocyanidins, phenolic acids, and flavonoids. Among detected compounds anthocyanins were identified as dominating. The exposure to Cd, dose- and duration-dependently, enhanced resorption and inhibited formation of the bone tissue resulting in its decreased mineralization. The administration of AMP under the exposure to 1 and 5 mgCd/kg diet provided important protection from this heavy metal-induced disturbances in the bone turnover and changes in the bone mineral status, and the beneficial impact of polyphenols resulted from their independent action and interaction with Cd. These findings suggest that consumption of Aronia melanocarpa polyphenols may play a role in prevention against female skeleton damage due to chronic exposure to Cd and that chokeberry represents the good natural plant candidate for further investigations of its prophylactic use under environmental exposure to this heavy metal. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Effect of an Extract from Aronia melanocarpa L. Berries on the Body Status of Zinc and Copper under Chronic Exposure to Cadmium: An In Vivo Experimental Study

PubMed Central

Borowska, Sylwia; Brzóska, Małgorzata M.; Gałażyn-Sidorczuk, Małgorzata; Rogalska, Joanna

2017-01-01

In an experimental model of low-level and moderate environmental human exposure to cadmium (Cd), it was investigated whether the consumption of a polyphenol-rich Aronia melanocarpa L. berries (chokeberries) extract (AE) may influence the body status of zinc (Zn) and copper (Cu). The bioelements’ apparent absorption, body retention, serum and tissue concentrations, total pool in internal organs, excretion, and the degree of binding to metallothionein were evaluated in female rats administered 0.1% aqueous AE or/and Cd in their diet (1 and 5 mg/kg) for 3–24 months. The consumption of AE alone had no influence on the body status of Zn and Cu. The extract administration at both levels of Cd treatment significantly (completely or partially) protected against most of the changes in the metabolism of Zn and Cu caused by this xenobiotic; however, it increased or decreased some of the Cd-unchanged indices of their body status. Based on the findings, it seems that rational amounts of chokeberry products may be included in the daily diet without the risk of destroying Zn and Cu metabolisms; however, their potential prophylactic use under exposure to Cd needs further study to exclude any unfavourable impact of these essential elements on the metabolism. PMID:29257101

Phytochemical profile of a blend of black chokeberry and lemon juice with cholinesterase inhibitory effect and antioxidant potential.

PubMed

Gironés-Vilaplana, Amadeo; Valentão, Patrícia; Andrade, Paula B; Ferreres, Federico; Moreno, Diego A; García-Viguera, Cristina

2012-10-15

In this study, black chokeberry concentrate was added (5% w/v) to lemon juice, since previous reports suggested potential health benefits of this blend. The phytochemical composition, antioxidant capacity (scavenging of DPPH, superoxide and hydroxyl radicals, and hypochlorous acid), and inhibitory activity against cholinesterase of the new blend were determined and compared with those of lemon juice and chokeberry in citric acid (5%). The chokeberry concentrate, rich in cyanidin-glycosides, quercetin derivatives, and 3-O-caffeoylquinic acid, and lemon juice, possessing flavones, flavanones, quercetin derivates, and hydroxycinnamic acids, were characterised. The new drink showed a higher antioxidant effect than the chokeberry or lemon controls for all the tested methods, except for hypochlorous acid, in which lemon juice displayed higher activity. Both the lemon juice and chokeberry controls inhibited acetylcholinesterase and butyrylcholinesterase, and this effect was increased in the new mixtures. The results of the different radical scavenging assays indicate that the lemon-black chokeberry (5% w/v) mixture was more antioxidative than the respective controls separately. Moreover, their inhibition of cholinesterase is of interest regarding neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease, or senile dementia. Copyright © 2012 Elsevier Ltd. All rights reserved.

Changes in chokeberry (Aronia melanocarpa L.) polyphenols during juice processing and storage.

PubMed

Wilkes, Kail; Howard, Luke R; Brownmiller, Cindi; Prior, Ronald L

2014-05-07

Chokeberries are an excellent source of polyphenols, but their fate during juice processing and storage is unknown. The stability of anthocyanins, total proanthocyanidins, hydroxycinnamic acids, and flavonols at various stages of juice processing and over 6 months of storage at 25 °C was determined. Flavonols, total proanthocyanidins, and hydroxycinnamic acids were retained in the juice to a greater extent than anthocyanins, with losses mostly due to removal of seeds and skins following pressing. Anthocyanins were extensively degraded by thermal treatments during which time levels of protocatechuic acid and phloroglucinaldehyde increased, and additional losses occurred following pressing. Flavonols, total proanthocyanidins, and hydroxycinnamic acids were well retained in juices stored for 6 months at 25 °C, whereas anthocyanins declined linearly. Anthocyanin losses during storage were paralleled by increased polymeric color values, indicating that the small amounts of anthocyanins remaining were present in large part in polymeric forms.

Up-regulation of tumor suppressor carcinoembryonic antigen-related cell adhesion molecule 1 in human colon cancer Caco-2 cells following repetitive exposure to dietary levels of a polyphenol-rich chokeberry juice.

PubMed

Bermúdez-Soto, María J; Larrosa, Mar; Garcia-Cantalejo, Jesús M; Espín, Juan C; Tomás-Barberan, Francisco A; García-Conesa, María T

2007-04-01

Consumption of berries and red fruits rich in polyphenols may contribute to the reduction of colon cancer through mechanisms not yet understood. In this study, we investigated the response of subconfluent Caco-2 cells (a human colon carcinoma model) to repetitive exposure (2 h a day for a 4-day period) of a subtoxic dose of a chokeberry (Aronia melanocarpa) juice containing mixed polyphenols. To mimic physiological conditions, we subjected the chokeberry juice to in vitro gastric and pancreatic digestion. The effects on viability, proliferation and cell cycle were determined, and changes in the expression of genes in response to the chokeberry treatment were screened using Affymetrix oligonucleotide microarrays. Exposure to the chokeberry juice inhibited Caco-2 cell proliferation by causing G(2)/M cell cycle arrest. We detected changes in the expression of a group of genes involved in cell growth and proliferation and cell cycle regulation, as well as those associated to colorectal cancer. A selection of these genes was further confirmed by quantitative RT-PCR. Among these, the tumor suppressor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), whose expression is known to be reduced in the majority of early adenomas and carcinomas, was up-regulated by the treatment both at the mRNA and protein levels (as shown by flow cytometry analysis). CEACAM1, with a significant regulatory role on cell proliferation of particular interest at early stages of cancer development, may be a potential target for chemoprevention by food components such as those present in polyphenol-rich fruits.

The multifunctionality of berries toward blood platelets and the role of berry phenolics in cardiovascular disorders.

PubMed

Olas, Beata

2017-09-01

Diet and nutrition have an important influence on the prophylaxis and progression of cardiovascular disease; one example is the inhibition of blood platelet functions by specific components of fruits and vegetables. Garlic, onion, ginger, dark chocolate and polyunsaturated fatty acids all reduce blood platelet aggregation. A number of fruits contain a range of cardioprotective antioxidants and vitamins, together with a large number of non-nutrient phytochemicals such as phenolic compounds, which may possess both antioxidant properties and anti-platelet activity. Fresh berries and berry extracts possess high concentrations of phenolic compounds, i.e. phenolic acid, stilbenoids, flavonoids and lignans. The aim of this review article is to provide an overview of current knowledge of the anti-platelet activity of berries, which form an integral part of the human diet. It describes the effects of phenolic compounds present in a number of berries, i.e. black chokeberries - aronia berries (Aronia melanocarpa), blueberries (Vaccinium myrtillus), cranberries (Vaccinium sect. Oxycoccus), sea buckthorn berries (Hippophae rhamnoides) and grapes (Vitis), as well as various commercial products from berries (i.e. juices), on platelets and underlying mechanisms. Studies show that the effects of berries on platelet activity are dependent on not only the concentrations of the phenolic compounds in the berries or the class of phenolic compounds, but also the types of berry and the form (fresh berry, juice or medicinal product). Different results indicate that berries may play a role in the prevention of cardiovascular disorders, but the development of well-controlled clinical studies with berries is encouraged.

Effects of Sambucus nigra and Aronia melanocarpa extracts on immune system disorders within diabetes mellitus.

PubMed

Badescu, Magda; Badulescu, Oana; Badescu, Laurentiu; Ciocoiu, Manuela

2015-04-01

The fruits of Aronia melanocarpa Elliot (Rosaceae), (black chokeberry), and Sambucus nigra L. (Caprifoliaceae), elderberries are rich in anthocyanins. Many studies have reported that anthocyanins are beneficial in diabetes due to their capacity to stimulate insulin secretion and reduce oxidative stress. The purpose of this study is to prove the biologically active properties of polyphenols extracted from S. nigra and A. melanocarpa fruit. The study also details the influence of plant polyphenols on immune system imbalances within diabetes mellitus. Polyphenolic extract was administered to Wistar rats 0.040 g/kg body every 2 d for 16 weeks. The absorbencies of all the solutions were determined using a V-550 Able Jasco UV-VIS spectrophotometer. The immunomodulatory capacity of vegetal extracts was assessed by studying cytokines TNF-α and IFN-γ through the ELISA method and fibrinogen values. At 48 h, the anti-inflammatory effects of S. nigra and A. melanocarpa substances have been revealed by an increase of the TNF-α and IFN-γ levels in the diabetic group protected by these extracts. Seventy-two hours post-administration of both substances in the diabetic groups, the TNF-α level returns to the values read 24 h after substance administration. The vegetal extracts limit the production of fibrinogen in the diabetic rats under polyphenolic protection, the values being highly significant compared with the diabetic group. Natural polyphenols extracted from S. nigra and A. melanocarpa modulate specific and non-specific immune defenses in insulin-deficiency diabetes and reduce the inflammatory status and self-sustained pancreatic insulitis.

Aronia melanocarpa extract suppresses the biotoxicity of homocysteine and its metabolite on the hemostatic activity of fibrinogen and plasma.

PubMed

Malinowska, Joanna; Babicz, Karolina; Olas, Beata; Stochmal, Anna; Oleszek, Wieslaw

2012-07-01

Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances, and it has been shown to have various biological activities. Berries of A. melanocarpa (chokeberry) have been supposed to be beneficial for the prevention of cardiovascular events. In this study the influence of aronia extract on the clot formation (using human plasma and purified fibrinogen) and the fibrin lysis during the model of hyperhomocysteinemia was investigated. Hyperhomocysteinemia was induced using a reduced form of Hcys (at final dose of 0.1mM) and the most reactive form of Hcys - its cyclic thioester, homocysteine thiolactone (HTL, 1 μM). The aim of our study in vitro was also to investigate the modifications of human plasma total proteins and the oxidative stress (by measuring the total antioxidant level - TAS) in plasma after incubation with Hcys, HTL and/or aronia extract. The biological properties of aronia extract were compared with the action of a well characterized antioxidative commercial polyphenol - resveratrol (3,4',5- trihydroxystilbene). The HTL, like its precursor, Hcys stimulated polymerization of fibrinogen. The results also demonstrated that Hcys (0.1mM) and HLT at lower doses than Hcys (1 μM) reduced the fibrin lysis in human plasma. Moreover, Hcys and HTL change the level of thiol and amino groups in plasma total proteins and induce the oxidative stress in plasma. Our results indicate that aronia extract reduced the biotoxicity action of Hcys and HTL on hemostatic properties of fibrinogen or plasma, suggesting its possible protective properties in hyperhomocysteinemia - induced cardiovascular diseases. Moreover, our results showed that the extract from berries of A. melanocarpa due to antioxidant action, significantly reduced the oxidative stress (measured by TAS) in plasma during the model of hyperhomocysteinemia. In the comparative studies, the extract from berries of A. melanocarpa and reseveratrol had similar protective properties. It gives hopes for development of diet supplements, which may be preventing thrombosis in pathological states where plasma procoagulant activity and oxidative stress are observed e.g. in hyperhomocysteinemia. Copyright © 2012 Elsevier Inc. All rights reserved.

The Seventh Biennial Berry Health Benefits Symposium.

PubMed

Seeram, Navindra P; Burton-Freeman, Britt

2018-01-24

Research advancing current scientific understanding of the health benefits of berries continues to increase. The Berry Health Benefits Symposium (BHBS) is a biennial meeting highlighting the most recent berry health benefits research from all over the world. Pismo Beach, California was the venue for the seventh biennial BHBS in 2017, and featured oral invited papers on heart health and healthy aging, gut/microbiome health, brain aging, inflammation, cancer prevention, berry special topics, technology and chemistry. These thematic health areas, while not exhaustive, characterize the state of berry health benefits science. The advancing field now encompasses human efficacy trials along with the most recent animal model and cell culture work elucidating mechanisms of action. Similar to past meetings, the research findings at the 2017 BHBS primarily focused on blackberries, blueberries, red raspberries, black raspberries, cranberries, and strawberries. However, research on other berry fruits, such as chokeberry (aronia berry), cloudberry, and bilberry were also covered. The BHBS continues to be a leading forum for interactions between scientists and berry industry stakeholders. The cluster of papers in this issue represents a snapshot of presentations at the 2017 BHBS, which support the positive biological effects of berries on human health and disease risk reduction.

Comparative anti-platelet and antioxidant properties of polyphenol-rich extracts from: berries of Aronia melanocarpa, seeds of grape and bark of Yucca schidigera in vitro.

PubMed

Olas, Beata; Wachowicz, Barbara; Tomczak, Anna; Erler, Joachim; Stochmal, Anna; Oleszek, Wieslaw

2008-02-01

The aim of the present study was to investigate and compare the anti-platelet action of extracts from three different plants: bark of Yucca schidigera, seeds of grape and berries of Aronia melanocarpa (chokeberry). Anti-platelet action of tested extracts was compared with action of well characterized antioxidative and anti-platelet commercial monomeric polyphenol-resveratrol. The effects of extracts on platelet adhesion to collagen, collagen-induced platelet aggregation and on the production of O2-* in resting platelets and platelets stimulated by a strong platelet agonist-thrombin were studied. The in vitro experiments have shown that all three tested extracts (5-50 microg/ml) rich in polyphenols reduce platelet adhesion, aggregation and generation of O2-* in blood platelets. Comparative studies indicate that all three plant extracts were found to be more reactive in reduction of platelet processes than the solution of pure resveratrol. The tested extracts due to their anti-platelet effects may play an important role as components of human diet in prevention of cardiovascular or inflammatory diseases, where blood platelets are involved.

Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets.

PubMed

Olas, B; Wachowicz, B; Nowak, P; Kedzierska, M; Tomczak, A; Stochmal, A; Oleszek, W; Jeziorski, A; Piekarski, J

2008-12-01

The antioxidant properties of extract from berries of Aronia melanocarpa (chokeberry) containing: anthocyanidines, phenolic acids and quercetine glycosides on oxidative/nitrative stress induced by peroxynitrite (ONOO(-), a powerful physiological oxidant, nitrating species and inflammatory mediator) in human blood platelets were studied in vitro. The extract from A. melanocarpa (5 - 50 microg/mL) significantly inhibited platelet protein carbonylation (measured by ELISA method) and thiol oxidation estimated with 5,5'-dithio-bis(2-nitro-benzoic acid) (DTNB) induced by peroxynitrite (0.1 mM) (IC(50)--35 microg/mL for protein carbonylation, and IC(50)--33 microg/mL for protein thiol oxidation). The tested extract only slightly reduced platelet protein nitration (measured by C- ELISA method). The extract also caused a distinct reduction of platelet lipid peroxidation induced by peroxynitrite. Moreover, in our preliminary experiments we observed that the extract (50 microg/mL) reduced oxidative/nitrative stress in blood platelets from patients with breast cancer. The obtained results indicate that in vitro the extract from A. melanocarpa has the protective effects against peroxynitrite-induced oxidative/nitrative damage to the human platelet proteins and lipids. The extract from A. melanocarpa seems to be also useful as an antioxidant in patients with breast cancer.

Current knowledge of Aronia melanocarpa as a medicinal plant.

PubMed

Valcheva-Kuzmanova, Stefka V; Belcheva, Anna

2006-01-01

Aronia melanocarpa, native to eastern North America, has become popular in Eastern Europe and Russia. Aronia melanocarpa fruits are one of the richest plant sources of phenolic substances, mainly anthocyanins--glycosides of cyanidin. Anthocyanins are water soluble pigments accounting for the dark blue and even black color of the fruits. Administered orally they can be absorbed as intact glycosides. Aronia melanocarpa fruit juice and anthocyanins derived from the fruits have been studied intensively for the last 15 years. Most of the effects of Aronia melanocarpa anthocyanins are due to their high antioxidative activity. Our investigations have demonstrated a remarkable hepatoprotective, a very good gastroprotective and a pronounced anti-inflammatory effect of Aronia melanocarpa fruit juice in rats as well as a bacteriostatic activity in vitro against Staphylococcus aureus and Escherichia coli and an antiviral activity against type A influenza virus. Research of other authors has demonstrated that Aronia melanocarpa anthocyanins can normalize the carbohydrate metabolism in diabetic patients and in streptozotocin-diabetic rats, have an in vitro antimutagenic activity and exhibit a distinct immunomodulatory activity in human lymphocyte cultures and in patients with breast cancer, suppress the growth of human HT-29 colon cancer cells, inhibit the N-nitrosamine formation in rats and decrease the toxicity and cumulation of cadmium in liver and kidneys. Currently, there are no data in literature about any unwanted and toxic effects of Aronia melanocarpa fruits, juice and extracts.

Aronia melanocarpa juice, a rich source of polyphenols, induces endothelium-dependent relaxations in porcine coronary arteries via the redox-sensitive activation of endothelial nitric oxide synthase.

PubMed

Kim, Jong Hun; Auger, Cyril; Kurita, Ikuko; Anselm, Eric; Rivoarilala, Lalainasoa Odile; Lee, Hyong Joo; Lee, Ki Won; Schini-Kerth, Valérie B

2013-11-30

This study examined the ability of Aronia melanocarpa (chokeberry) juice, a rich source of polyphenols, to cause NO-mediated endothelium-dependent relaxations of isolated coronary arteries and, if so, to determine the underlying mechanism and the active polyphenols. A. melanocarpa juice caused potent endothelium-dependent relaxations in porcine coronary artery rings. Relaxations to A. melanocarpa juice were minimally affected by inhibition of the formation of vasoactive prostanoids and endothelium-derived hyperpolarizing factor-mediated responses, and markedly reduced by N(ω)-nitro-l-arginine (endothelial NO synthase (eNOS) inhibitor), membrane permeant analogs of superoxide dismutase and catalase, PP2 (Src kinase inhibitor), and wortmannin (PI3-kinase inhibitor). In cultured endothelial cells, A. melanocarpa juice increased the formation of NO as assessed by electron paramagnetic resonance spectroscopy using the spin trap iron(II)diethyldithiocarbamate, and reactive oxygen species using dihydroethidium. These responses were associated with the redox-sensitive phosphorylation of Src, Akt and eNOS. A. melanocarpa juice-derived fractions containing conjugated cyanidins and chlorogenic acids induced the phosphorylation of Akt and eNOS. The present findings indicate that A. melanocarpa juice is a potent stimulator of the endothelial formation of NO in coronary arteries; this effect involves the phosphorylation of eNOS via the redox-sensitive activation of the Src/PI3-kinase/Akt pathway mostly by conjugated cyanidins and chlorogenic acids. Copyright © 2013. Published by Elsevier Inc.

The effect of dietary supplementation with dried fruit and vegetable pomaces on production parameters and meat quality in fattening pigs.

PubMed

Pieszka, Marek; Szczurek, Paulina; Bederska-Łojewska, Dorota; Migdał, Władysław; Pieszka, Magdalena; Gogol, Piotr; Jagusiak, Wojciech

2017-04-01

The presence of biologically active substances in feed mixture is discussed to have beneficial effect on animals' health and products. The purpose of the study was to determine the effect of dietary supplementation with dried apple, chokeberry, black currant, strawberry and carrot pomaces on production parameters and meat quality in fattening pigs. The use of dried pomaces of chokeberry showed tendencies for increased feed intake and reduced fattening period. The dried pomaces had no impact on saturated and monounsaturated fatty acids profile in meat, however in some groups an elevated level of polyunsaturated fatty acids from n-3 family and a decline in total cholesterol level was observed (P≤0.05). The highest oxidative stability and vitamin E content was found after supplementation with black currant (P≤0.05). Summarizing, the used dried pomaces improved several parameters related to meat quality, what might positively influence consumers' health. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Aronia melanocarpa (chokeberry) polyphenol-rich extract improves antioxidant function and reduces total plasma cholesterol in apolipoprotein E knockout mice.

PubMed

Kim, Bohkyung; Ku, Chai Siah; Pham, Tho X; Park, Youngki; Martin, Derek A; Xie, Liyang; Taheri, Rod; Lee, Jiyoung; Bolling, Bradley W

2013-05-01

We hypothesized that a polyphenol-rich chokeberry extract (CBE) would modulate hepatic lipid metabolism and improve antioxidant function in apolipoprotein E knockout (apoE(-/-)) mice. ApoE(-/-) mice were fed diets containing 15% fat with 0.2% cholesterol alone or supplemented with 0.005% or 0.05% CBE for 4 weeks. CBE polyphenol content was determined by the total phenols, 4-dimethylaminocinnamaldehyde, and ultra high-performance liquid chromatography-mass spectrometry methods. The 0.05% CBE diet provided mice with mean daily doses of 1.2 mg gallic acid equivalents of total phenols, 0.19 mg anthocyanins, 0.17 mg phenolic acids, 0.06 mg proanthocyanidins (as catechin-equivalents), and 0.02 mg flavonols. The 0.05% CBE group had 12% less plasma total cholesterol concentrations than the control. Despite the hypocholesterolemic effect of CBE, hepatic mRNA levels of low-density lipoprotein receptor, hydroxyl-3-methylglutaryl coenzyme A reductase and cholesterol 7α-hydroxylase in CBE-fed mice were not significantly different from controls. Dietary CBE did not alter hepatic lipid content or the hepatic expression of genes involved in lipogenesis and fatty acid β-oxidation such as fatty acid synthase, carnitine palmitoyltransferase 1 and acyl-CoA oxidase. Plasma paraoxonase and catalase activities were significantly increased in mice fed 0.05% CBE. Both CBE diets increased hepatic glutathione peroxidase (GPx) activity but the 0.05% CBE group had 24% less proximal intestine GPx activity relative to controls. Thus, dietary CBE lowered total cholesterol and improved plasma and hepatic antioxidant function at nutritionally-relevant doses in apoE(-/-) mice. Furthermore, the CBE cholesterol-lowering mechanism in apoE(-/-) mice was independent of hepatic expression of genes involved in cholesterol metabolism. Copyright © 2013 Elsevier Inc. All rights reserved.

[Contents of heavy metals, nitrate (V), and nitrate (III) in fruits of elderberry and black chokeberry depending on harvest site and vegetation period].

PubMed

Ognik, Katarzyna; Rusinek, Elzbieta; Sembratowicz, Iwona; Truchliński, Jerzy

2006-01-01

Lead, cadmium, nitrate V and nitrate III contents were determined in elderberry and black chokeberry fruits harvested in Lublin region. Samples were taken from areas that were potentially exposed and not exposed to pollution. Fruits analyzed in 2003 and 2004 harvested from area exposed to pollution were characterized by higher lead concentration (0.043-0.098 mg kg(-1) fresh mass and 0.048-0.081 mg kg(-1) fresh mass, respectively) than those collected from potentially not polluted area. Levels of tested metals in fruits harvested from both areas in 2004 were fairly similar to those from 2003. However, cadmium content in all collected fruits from both areas (in both vegetation periods) appeared to be high and much exceeded its permissible value (0.03 mg kg(-1) fresh mass). Levels of nitrate V was low and similar in fruits harvested in both seasons from both areas not exceeding 3.4 mg kg(-1) fresh mass. Content of nitrate III was also low and oscillated within the range 0.68-0.89 mg kg(-1) fresh mass.

Susceptibility of anthocyanins to ex vivo degradation in human saliva

PubMed Central

Kamonpatana, Kom; Giusti, M. Mónica; Chitchumroonchokchai, Chureeporn; MorenoCruz, Maria; Riedl, Ken M.; Kumar, Purnima; Failla, Mark L.

2013-01-01

Some fruits and their anthocyanin-rich extracts have been reported to exhibit chemopreventive activity in the oral cavity. Insights regarding oral metabolism of anthocyanins remain limited. Anthocyanin-rich extracts from blueberry, chokeberry, black raspberry, red grape, and strawberry were incubated ex vivo with human saliva from 14 healthy subjects. All anthocyanins were partially degraded in saliva. Degradation of chokeberry anthocyanins in saliva was temperature dependent and decreased by heating saliva to 80 °C and after removal of cells. Glycosides of delphinidin and petunidin were more susceptible to degradation than those of cyanidin, pelargonidin, peonidin and malvidin in both intact and artificial saliva. Stability of di- and tri-saccharide conjugates of anthocyanidins slightly, but significantly, exceeded that of monosaccharide compounds. Ex vivo degradation of anthocyanins in saliva was significantly decreased after oral rinsing with antibacterial chlorhexidine. These results suggest that anthocyanin degradation in the mouth is structure-dependent and largely mediated by oral microbiota. PMID:22868153

Quality Characteristics and Antioxidant Activity of Yogurt Supplemented with Aronia (Aronia melanocarpa) Juice.

PubMed

Nguyen, Linh; Hwang, Eun-Sun

2016-12-01

We investigated the quality characteristics and antioxidant activities of yogurt supplemented with 1%, 2%, and 3% aronia juice and fermented for 24 h at 37°C. The total acidity increased with increasing levels of aronia juice and incubation time. Lightness and yellowness of the yogurt decreased, but redness increased, with increasing aronia juice content and incubation time. The number of lactic acid bacteria (LAB) increased with increased incubation time, and yogurt containing 2% and 3% aronia juice showed higher LAB counts than 1% aroinia juice-supplemented yogurt. The total polyphenol and flavonoid contents increased proportionally with increasing levels of aronia juice. Antioxidant activity of aronia-containing yogurt was significantly higher than that of the control and increased proportionally with aronia juice concentration. Yogurt with 2% aronia juice had the best taste ( P <0.05). Aronia juice may be a useful additive for improving the taste and antioxidant potential of yogurt.

Quality Characteristics and Antioxidant Activity of Yogurt Supplemented with Aronia (Aronia melanocarpa) Juice

PubMed Central

Nguyen, Linh; Hwang, Eun-Sun

2016-01-01

We investigated the quality characteristics and antioxidant activities of yogurt supplemented with 1%, 2%, and 3% aronia juice and fermented for 24 h at 37°C. The total acidity increased with increasing levels of aronia juice and incubation time. Lightness and yellowness of the yogurt decreased, but redness increased, with increasing aronia juice content and incubation time. The number of lactic acid bacteria (LAB) increased with increased incubation time, and yogurt containing 2% and 3% aronia juice showed higher LAB counts than 1% aroinia juice-supplemented yogurt. The total polyphenol and flavonoid contents increased proportionally with increasing levels of aronia juice. Antioxidant activity of aronia-containing yogurt was significantly higher than that of the control and increased proportionally with aronia juice concentration. Yogurt with 2% aronia juice had the best taste (P<0.05). Aronia juice may be a useful additive for improving the taste and antioxidant potential of yogurt. PMID:28078255

The Mechanism of the Osteoprotective Action of a Polyphenol-Rich Aronia melanocarpa Extract during Chronic Exposure to Cadmium is Mediated by the Oxidative Defense System.

PubMed

Brzóska, Malgorzata M; Rogalska, Joanna; Roszczenko, Alicja; Galazyn-Sidorczuk, Malgorzata; Tomczyk, Michal

2016-05-01

Recently, we demonstrated in a rat model that consumption of a polyphenol-rich extract obtained from the berries of Aronia melanocarpa could protect from cadmium-induced disorders in bone turnover and changes in bone mineral status. The aim of this study was to investigate whether the osteoprotective effect of this extract is mediated by the oxidative defense system. Enzymatic and nonenzymatic antioxidants, total antioxidative and oxidative status, hydrogen peroxide, and markers of oxidative protein, lipid, and DNA damage were determined in bone tissue at the distal femoral epiphysis of female Wistar rats receiving 0.1 % aqueous A. melanocarpa extract (prepared from the lyophilized commercial extract containing 65.74 % of polyphenols) as the only drinking fluid and/or cadmium in the diet (1 and 5 mg/kg) for 3, 10, 17, and 24 months. The total oxidative and antioxidative status of the serum was also evaluated. The administration of A. melanocarpa extract provided significant protection from cadmium-induced oxidative stress in the bone and serum, and from lipid peroxidation and oxidative damage to the protein and DNA in the bone tissue. Numerous correlations were noted between indices of the oxidative/antioxidative bone status and markers of bone metabolism previously assayed in the animals receiving A. melanocarpa extract. The results allow the conclusion that the ability of A. melanocarpa extract to mediate the oxidative defense system and prevent oxidative modifications of protein, lipid, and DNA in the bone tissue plays an important role in its osteoprotective action under exposure to cadmium. The findings provide further evidence supporting our suggestion that chokeberry may be a promising natural agent for protection against the toxic action of cadmium in women chronically exposed to this metal. Georg Thieme Verlag KG Stuttgart · New York.

Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.

PubMed

Wei, Jie; Zhang, Guokun; Zhang, Xiao; Xu, Dexin; Gao, Jun; Fan, Jungang; Zhou, Zhiquan

2017-07-26

Aging is the greatest risk factor for most neurodegenerative diseases, which is associated with decreasing cognitive function and significantly affecting life quality in the elderly. Computational analysis suggested that 4 anthocyanins from chokeberry fruit increased Klotho (aging-suppressor) structural stability, so we hypothesized that chokeberry anthocyanins could antiaging. To explore the effects of anthocyanins treatment on brain aging, mice treated with 15 or 30 mg/kg anthocyanins by gavage and injected D-galactose accelerated aging per day. After 8 weeks, cognitive and noncognitive components of behavior were determined. Our studies showed that anthocyanins blocked age-associated cognitive decline and response capacity in senescence accelerated mice. Furthermore, mice treated with anthocyanins-supplemented showed better balance of redox systems (SOD, GSH-PX, and MDA) in all age tests. Three major monoamines were norepinephrine, dopamine, and 5-hydroxytryptamine, and their levels were significantly increased; the levels of inflammatory cytokines (COX2, TGF-β1, and IL-1) transcription and DNA damage were decreased significantly in brains of anthocyanins treated mice compared to aged models. The DNA damage signaling pathway was also regulated with anthocyanins. Our results suggested that anthocyanins was a potential approach for maintaining thinking and memory in aging mice, possibly by regulating the balance of redox system and reducing inflammation accumulation, and the most important factor was inhibiting DNA damage.

Low-Dose Aronia melanocarpa Concentrate Attenuates Paraquat-Induced Neurotoxicity

PubMed Central

Case, A. J.; Agraz, D.; Ahmad, I. M.; Zimmerman, M. C.

2016-01-01

Herbicides containing paraquat may contribute to the pathogenesis of neurodegenerative disorders such as Parkinson's disease. Paraquat induces reactive oxygen species-mediated apoptosis in neurons, which is a primary mechanism behind its toxicity. We sought to test the effectiveness of a commercially available polyphenol-rich Aronia melanocarpa (aronia berry) concentrate in the amelioration of paraquat-induced neurotoxicity. Considering the abundance of antioxidants in aronia berries, we hypothesized that aronia berry concentrate attenuates the paraquat-induced increase in reactive oxygen species and protects against paraquat-mediated neuronal cell death. Using a neuronal cell culture model, we observed that low doses of aronia berry concentrate protected against paraquat-mediated neurotoxicity. Additionally, low doses of the concentrate attenuated the paraquat-induced increase in superoxide, hydrogen peroxide, and oxidized glutathione levels. Interestingly, high doses of aronia berry concentrate increased neuronal superoxide levels independent of paraquat, while at the same time decreasing hydrogen peroxide. Moreover, high-dose aronia berry concentrate potentiated paraquat-induced superoxide production and neuronal cell death. In summary, aronia berry concentrate at low doses restores the homeostatic redox environment of neurons treated with paraquat, while high doses exacerbate the imbalance leading to further cell death. Our findings support that moderate levels of aronia berry concentrate may prevent reactive oxygen species-mediated neurotoxicity. PMID:26770655

Low-Dose Aronia melanocarpa Concentrate Attenuates Paraquat-Induced Neurotoxicity.

PubMed

Case, A J; Agraz, D; Ahmad, I M; Zimmerman, M C

2016-01-01

Herbicides containing paraquat may contribute to the pathogenesis of neurodegenerative disorders such as Parkinson's disease. Paraquat induces reactive oxygen species-mediated apoptosis in neurons, which is a primary mechanism behind its toxicity. We sought to test the effectiveness of a commercially available polyphenol-rich Aronia melanocarpa (aronia berry) concentrate in the amelioration of paraquat-induced neurotoxicity. Considering the abundance of antioxidants in aronia berries, we hypothesized that aronia berry concentrate attenuates the paraquat-induced increase in reactive oxygen species and protects against paraquat-mediated neuronal cell death. Using a neuronal cell culture model, we observed that low doses of aronia berry concentrate protected against paraquat-mediated neurotoxicity. Additionally, low doses of the concentrate attenuated the paraquat-induced increase in superoxide, hydrogen peroxide, and oxidized glutathione levels. Interestingly, high doses of aronia berry concentrate increased neuronal superoxide levels independent of paraquat, while at the same time decreasing hydrogen peroxide. Moreover, high-dose aronia berry concentrate potentiated paraquat-induced superoxide production and neuronal cell death. In summary, aronia berry concentrate at low doses restores the homeostatic redox environment of neurons treated with paraquat, while high doses exacerbate the imbalance leading to further cell death. Our findings support that moderate levels of aronia berry concentrate may prevent reactive oxygen species-mediated neurotoxicity.

Effects of Different Growing Regions on Quality Characteristics, Bioactive Compound Contents, and Antioxidant Activity of Aronia (Aronia melanocarpa) in Korea.

PubMed

Hwang, Eun-Sun; Thi, Nhuan Do

2016-09-01

The objective of this study was to determine the effects of different growing regions on quality characteristics, total bioactive compound contents, and in vitro antioxidant activity in aronia. Aronia grown in 3 different regions (Sangjoo, Ulju, and Youngcheon) in Korea was obtained and used fresh or as a freeze-dried powder. No statistically significant differences were observed for moisture, ash, crude lipid, and crude protein contents in aronia sampled from the 3 different regions. Aronia grown in Sangjoo had the highest total acid content and the lowest sugar content and pH value. Conversely, aronia grown in Youngcheon possessed the lowest total acid content and the highest sugar content and pH value. Aronia grown in Sangjoo possessed relatively high levels of polyphenols, flavonoids, and anthocyanins, as well as high antioxidant activity in comparison with aronia produced in other regions. Aronia grown in Youngcheon scored the highest for taste and overall acceptability in sensory evaluations, which may be related to the high sugar content and pH, and the low total acidity of the fruits. It is possible that higher sugar contents and pH, and lower total acidity in the aronia grown in Youngcheon result in more preferable sensory characteristics. However, they also contain relatively low levels of total polyphenols, flavonoids, and anthocyanins, and have low antioxidant activity as measured by 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assays.

Effects of Different Growing Regions on Quality Characteristics, Bioactive Compound Contents, and Antioxidant Activity of Aronia (Aronia melanocarpa) in Korea

PubMed Central

Hwang, Eun-Sun; Thi, Nhuan Do

2016-01-01

The objective of this study was to determine the effects of different growing regions on quality characteristics, total bioactive compound contents, and in vitro antioxidant activity in aronia. Aronia grown in 3 different regions (Sangjoo, Ulju, and Youngcheon) in Korea was obtained and used fresh or as a freeze-dried powder. No statistically significant differences were observed for moisture, ash, crude lipid, and crude protein contents in aronia sampled from the 3 different regions. Aronia grown in Sangjoo had the highest total acid content and the lowest sugar content and pH value. Conversely, aronia grown in Youngcheon possessed the lowest total acid content and the highest sugar content and pH value. Aronia grown in Sangjoo possessed relatively high levels of polyphenols, flavonoids, and anthocyanins, as well as high antioxidant activity in comparison with aronia produced in other regions. Aronia grown in Youngcheon scored the highest for taste and overall acceptability in sensory evaluations, which may be related to the high sugar content and pH, and the low total acidity of the fruits. It is possible that higher sugar contents and pH, and lower total acidity in the aronia grown in Youngcheon result in more preferable sensory characteristics. However, they also contain relatively low levels of total polyphenols, flavonoids, and anthocyanins, and have low antioxidant activity as measured by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assays. PMID:27752502

Identification and characterization of a dipeptidyl peptidase IV inhibitor from aronia juice

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kozuka, Miyuki; Yamane, Takuya, E-mail: t-yamane@pharm.hokudai.ac.jp; Nakano, Yoshihisa

Aronia berries have many potential effects on health, including an antioxidant effect, effect for antimutagenesis, hepatoprotection and cardioprotection, an antidiabetic effect and inhibition of cancer cell proliferation. Previous human studies have shown that aronia juice may be useful for treatment of obesity disorders. In this study, we found that aronia juice has an inhibitory effect against dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5). DPP IV is a peptidase that cleaves the N-terminal region of incretins such as glucagon-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). Inactivation of incretins by DPP IV induces reduction of insulin secretion. Furthermore, we identified thatmore » cyanidin 3, 5-diglucoside as the DPP IV inhibitor in aronia juice. DPP IV was inhibited more strongly by cyanidin 3, 5-diglucoside than by cyanidin and cyanidin 3-glucoside. The results suggest that DPP IV is inhibited by cyanidin 3, 5-diglucoside present in aronia juice. The antidiabetic effect of aronia juice may be mediated through DPP IV inhibition by cyanidin 3, 5-diglucoside. - Highlights: • DPP IV activity is inhibited by aronia juice. • DPP IV inhibitor is cyanidin 3, 5-diglucoside in aronia juice. • DPP IV is inhibited by cyanidin 3, 5-diglucoside more than cyanidin and cyanidin 3-glucoside.« less

Phytochemical characterization of several hawthorn (Crataegus spp.) species sampled from the Eastern Mediterranean region of Turkey.

PubMed

Calişkan, Oğuzhan; Gündüz, Kazim; Serçe, Sedat; Toplu, Celil; Kamiloğlu, Onder; Sengül, Memnune; Ercişli, Sezai

2012-01-01

We evaluated the total phenolic content, antioxidant capacity as well as antioxidant activity of five Crataegus species (A1, A2, Y1, Y2, Y4 accessions of Crataegus aronia var. aronia; B2, B3, B5, B6, B7, B9, Y5 accessions of C. aronia var. dentata; B10 accession of C. aronia var. minuta; Y3 accession of Crataegus orientalis var. orientalis and A3 accession of Crataegus monogyna subsp. azarella). Antioxidant activity and total phenolic content of fruits were determined by β-carotene bleaching and Folin-Ciocalteu assays. Antioxidant capacity was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. C. monogyna subsp. azarella had the highest total phenol, antioxidant activity and antioxidant capacity of 55.2 mg gallic acid equivalents (GAE)/g dry weight (DW), 81.9% and 31.2%, respectively. C. aronia var. aronia was found to have the lowest total phenolic content (35.7 mg GAE/g DW). The antioxidant activities of fruit extracts increased in the order of C. orientalis var. orientalis < C. aronia var. minuta < C. aronia var. dentata < C. aronia var. aronia < C. monogyna subsp. azarella according to β-carotene/linoleic acid assay. In recent years, C. aronia var. dentata has gained importance as a commercial species in this region. B3 and B7 accessions had fruit weight more than 14 g, and considerable total phenol content, antioxidant activity and antioxidant capacity. This investigation shows the potential value of hawthorn fruit species as a good source of natural antioxidants and that consumption of hawthorn fruit or its products may contribute substantial amounts of antioxidants to the diet.

Phytochemical characterization of several hawthorn (Crataegus spp.) species sampled from the Eastern Mediterranean region of Turkey

PubMed Central

Çalişkan, Oğuzhan; Gündüz, Kazim; Serçe, Sedat; Toplu, Celil; Kamiloğlu, Önder; Şengül, Memnune; Ercişli, Sezai

2012-01-01

Background: We evaluated the total phenolic content, antioxidant capacity as well as antioxidant activity of five Crataegus species (A1, A2, Y1, Y2, Y4 accessions of Crataegus aronia var. aronia; B2, B3, B5, B6, B7, B9, Y5 accessions of C. aronia var. dentata; B10 accession of C. aronia var. minuta; Y3 accession of Crataegus orientalis var. orientalis and A3 accession of Crataegus monogyna subsp. azarella). Materials and Methods: Antioxidant activity and total phenolic content of fruits were determined by β-carotene bleaching and Folin–Ciocalteu assays. Antioxidant capacity was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: C. monogyna subsp. azarella had the highest total phenol, antioxidant activity and antioxidant capacity of 55.2 mg gallic acid equivalents (GAE)/g dry weight (DW), 81.9% and 31.2%, respectively. C. aronia var. aronia was found to have the lowest total phenolic content (35.7 mg GAE/g DW). The antioxidant activities of fruit extracts increased in the order of C. orientalis var. orientalis < C. aronia var. minuta < C. aronia var. dentata < C. aronia var. aronia < C. monogyna subsp. azarella according to β-carotene/linoleic acid assay. In recent years, C. aronia var. dentata has gained importance as a commercial species in this region. B3 and B7 accessions had fruit weight more than 14 g, and considerable total phenol content, antioxidant activity and antioxidant capacity. Conclusion: This investigation shows the potential value of hawthorn fruit species as a good source of natural antioxidants and that consumption of hawthorn fruit or its products may contribute substantial amounts of antioxidants to the diet. PMID:22438658

Drinking of chokeberry juice from the ecological farm Dzieciolowo and distensibility of brachial artery in men with mild hypercholesterolemia.

PubMed

Poreba, Rafal; Skoczynska, Anna; Gac, Pawel; Poreba, Malgorzata; Jedrychowska, Iwona; Affelska-Jercha, Anna; Turczyn, Barbara; Wojakowska, Anna; Oszmianski, Jan; Andrzejak, Ryszard

2009-01-01

The aim of the study was to estimate the influence of drinking chokeberry juice on the endothelial function in men with mild hypercholesterolemia. It was shown that chronic treatment with flavonoids improves vascular function and reduces cardiovascular remodelling by increasing NO release from endothelial cells. A group of 35 men diagnosed with mild hypercholesterolemia (mean age: 53.9 +-5.8 years), with no earlier pharmacological treatment, were enrolled to the study. In all men, assessment of endothelial function, and serum lipids level were carried out at four time points: at the beginning of the studies, after 6 weeks of regular drinking of chokeberry juice, after 6 weeks without drinking the juice, then after repeated 6 weeks of drinking chokeberry juice. During the study, significant decreases in serum total cholesterol, LDL cholesterol and triglycerides levels were observed. A statistically significant increase in serum nitric oxide (NO) concentration and in the flow mediated dilatation (FMD) were observed. At the beginning, FMD > or = 7 % was present in 13 of 35 subjects (37.1 %). After 6 weeks of regular chokeberry juice drinking, FMD > or = 7 % was present in 29 of 35 subjects (82.9 %). However, after 6 weeks abstaining from drinking the juice and repeated exposure to 6 weeks drinking of chokeberry juice, FMD > or = 7 % was present in all studied subjects. Regular drinking of chokeberry juice has a beneficial effect on endothelial function and lipid metabolism in men with mild hypercholesterolemia.

Protective and therapeutic effects of Crataegus aronia in non-alcoholic fatty liver disease.

PubMed

Al Humayed, Suliman

2017-02-01

We evaluated the potential preventive and therapeutic effects of Crataegus aronia (C. aronia) in NAFLD induced by high-fat diet (HFD) in rat models. Protective effect of Crataegus aronia or simvastatin was investigated in Wistar rats fed either low-fat diet (LFD) or HFD. Liver histopathological examinations confirmed the development of NAFLD in rats fed HFD. In both protective and therapeutic treatments, C. aronia significantly reduced liver index (3.85 ± 0.21% in HFD plus aronia group versus 6.22 ± 0.58% in HFD model group), increased the HDL-cholesterol and reduced the LDL-cholesterol in blood. The hawthorn plant also significantly ameliorated oxidative stress biomarker (p < 0.002) and liver enzymes (p < 0.0001) that indicate liver damage. C. aronia exhibits therapeutic and protective effects on NAFLD in an animal model possibly by its lipid lowering and antioxidant effects; thus, may offer therapeutic potential in humans.

Effects of a natural extract of Aronia Melanocarpa berry on endothelial cell nitric oxide production.

PubMed

Varela, Claudia Elena; Fromentin, Emilie; Roller, Marc; Villarreal, Francisco; Ramirez-Sanchez, Israel

2016-08-01

The effects of acute and chronic treatment with Aronia extracts on NO production and endothelial nitric oxide synthase (eNOS) phosphorylation in bovine coronary artery endothelial cells were investigated. Acute time-course and concentration-response experiments were performed to determine the time and concentration at which Aronia induced maximal NO synthesis and eNOS phosphorylation. The findings indicate that relatively low concentrations (0.1 μg/mL) of Aronia extract significantly induced NO synthesis and eNOS phosphorylation after 10 min of treatment. Increased sensitivity of eNOS and a significant increase in NO synthesis resulted from longer-term stimulation with Aronia (48 hr) and an acute re-treatment of the cells (10 min). These in vitro results may be translated into potential future clinical applications where Aronia extracts may be used for prevention and coadjuvant treatment of cardiovascular diseases via increases in endothelial NO synthesis and related improvements in vascular functions. Given the dose-response effect of Aronia extract in vitro and metabolism of polyphenols that occurs in humans, dose-response studies would be necessary to define the optimal daily amount to be consumed.

Effects of a natural extract of Aronia Melanocarpa berry on endothelial cell nitric oxide production

PubMed Central

Varela, Claudia Elena; Fromentin, Emilie; Roller, Marc; Villarreal, Francisco; Ramirez-Sanchez, Israel

2015-01-01

The effects of acute and chronic treatment with Aronia extracts on NO production and endothelial nitric oxide synthase (eNOS) phosphorylation in bovine coronary artery endothelial cells were investigated. Acute time-course and concentration-response experiments were performed to determine the time and concentration at which Aronia induced maximal NO synthesis and eNOS phosphorylation. The findings indicate that relatively low concentrations (0.1 μg/mL) of Aronia extract significantly induced NO synthesis and eNOS phosphorylation after 10 min of treatment. Increased sensitivity of eNOS and a significant increase in NO synthesis resulted from longer-term stimulation with Aronia (48 hr) and an acute re-treatment of the cells (10 min). PRACTICAL APPLICATIONS These in vitro results may be translated into potential future clinical applications where Aronia extracts may be used for prevention and coadjuvant treatment of cardiovascular diseases via increases in endothelial NO synthesis and related improvements in vascular functions. Given the dose-response effect of Aronia extract in vitro and metabolism of polyphenols that occurs in humans, dose-response studies would be necessary to define the optimal daily amount to be consumed. PMID:27616799

Aronia berry polyphenol consumption reduces plasma total and low-density lipoprotein cholesterol in former smokers without lowering biomarkers of inflammation and oxidative stress: a randomized controlled trial.

PubMed

Xie, Liyang; Vance, Terrence; Kim, Bohkyung; Lee, Sang Gil; Caceres, Christian; Wang, Ying; Hubert, Patrice A; Lee, Ji-Young; Chun, Ock K; Bolling, Bradley W

2017-01-01

Former smokers are at increased risk for cardiovascular disease. We hypothesized that dietary aronia polyphenols would reduce biomarkers of cardiovascular disease risk, inflammation, and oxidative stress in former smokers. We also determined the extent these effects were associated with polyphenol bioavailability. A 12-week, randomized, placebo-controlled trial was conducted in 49 healthy adult former smokers (n = 24/placebo, n = 25/aronia) to evaluate if daily consumption of 500 mg aronia extract modulated plasma lipids, blood pressure, biomarkers of inflammation and oxidative stress, and lipid transport genes of peripheral blood mononuclear cells. The primary outcome was change in low-density lipoprotein cholesterol (LDL-C) from baseline, and multivariate correlation analysis was performed to determine if changes in lipids were associated with urinary polyphenol excretion. Aronia consumption reduced fasting plasma total cholesterol by 8% (P = .0140), LDL-C by 11% (P = .0285), and LDL receptor protein in peripheral blood mononuclear cells (P = .0036) at 12 weeks compared with the placebo group. Positive changes in the urinary polyphenol metabolites peonidin-3-O-galactoside, 3-(4-hydroxyphenyl) propionic acid, and unmetabolized anthocyanin cyanidin-3-O-galactoside were associated with lower plasma total cholesterol and LDL-C in the aronia group. Aronia consumption did not change blood pressure or biomarkers of inflammation and oxidative stress. Aronia polyphenols reduced total and LDL-C in former smokers but did not improve biomarkers of oxidative stress and chronic inflammation. The cholesterol-lowering activity of aronia extract was most closely associated with urinary levels of cyanidin-3-O-galactoside and peonidin-3-O-galactoside, its methylated metabolite. This trial was registered at ClinicalTrials.gov as NCT01541826. Copyright © 2016 Elsevier Inc. All rights reserved.

Salal (Gaultheria shallon) and aronia (Aronia melanocarpa) fruits from Orkney: Phenolic content, composition and effect of wine-making.

PubMed

McDougall, G J; Austin, C; Van Schayk, E; Martin, P

2016-08-15

The polyphenol content and composition of salal and aronia fruits from plants established in Orkney was examined. The composition of the salal fruits has not previously been recorded, and they contained anthocyanins, flavonols, hydroxycinnamates and proanthocyanins. The aronia fruits contained anthocyanins, hydroxycinnamates and flavonols as previously described. Although salal fruits had half the anthocyanin content of aronia fruits, salal wine had higher anthocyanin content, probably due to the relative stability of diglycoside pentose anthocyanins. The wines contained components suggestive of anthocyanin and flavonol degradation, but there was no consistent pattern to stability within phenolic sub-classes. Indeed, the wine made from equal amounts of salal and aronia fruits had patterns of recovery of individual phenolic components which could not be predicted from recoveries in wines from single fruits. This strongly suggests that stability of individual phenolic constituents during wine-making is influenced by the presence and relative stability of other components. Copyright © 2016 Elsevier Ltd. All rights reserved.

Genetic relationships among some hawthorn (Crataegus spp.) species and genotypes.

PubMed

Yilmaz, Kadir Ugurtan; Yanar, Makbule; Ercisli, Sezai; Sahiner, Hatice; Taskin, Tuncer; Zengin, Yasar

2010-10-01

The genus Crataegus is well distributed in Turkey as a wild plant, with numerous, inherently variable species and genotypes. RAPD markers were used to study 17 hawthorn genotypes belonging to Crataegus monogyna ssp. monogyna Jacq (2 genotypes), C. monogyna ssp. azarella Jacq (1), Crataegus pontica K.Koch (3), Crataegus orientalis var. orientalis Pallas Ex Bieb (3), Crataegus pseudoheterophylla Pojark (1), Crataegus aronia var. dentata Browicz (1), C. aronia var. aronia Browicz (4), and Crateagus x bornmuelleri Zabel (2). The 10 RAPD primers produced 72 polymorphic bands (88% polymorphism). A dendrogram based on Jaccard's index included four major groups and one outgroup according to taxa. The lowest genetic variability was observed within C. aronia var. aronia genotypes. The study demonstrated that RAPD analysis is efficient for genotyping wild-grown hawthorns.

Bioactive Compound Contents and Antioxidant Activity in Aronia (Aronia melanocarpa) Leaves Collected at Different Growth Stages.

PubMed

Thi, Nhuan Do; Hwang, Eun-Sun

2014-09-01

The bioactive compounds and antioxidant activity of aronia leaves at different stages of maturity were identified and evaluated. Young and old leaves were approximately 2 months of age and 4 months of age, respectively. The young leaves contained more polyphenols and flavonoids than the old leaves. Three phenolic compounds (i.e., chlorogenic acid, p-coumaric acid, and rutin) were detected by HPLC. Antioxidant activity was measured using 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical, and superoxide anion radical scavenging assays. The reducing power of aronia leaf extracts increased in a concentration-dependent manner (0~100 μg/mL). The antioxidant activity of the 80% ethanol extract was greater than that of distilled water extract. The high phenolic compound content indicated that these compounds contribute to antioxidant activity. The overall results indicate that aronia leaves contain bioactive compounds, and that younger aronia leaves may be more favorable for extracting antioxidative ingredients because they contain more polyphenols.

Bioactive Compound Contents and Antioxidant Activity in Aronia (Aronia melanocarpa) Leaves Collected at Different Growth Stages

PubMed Central

Thi, Nhuan Do; Hwang, Eun-Sun

2014-01-01

The bioactive compounds and antioxidant activity of aronia leaves at different stages of maturity were identified and evaluated. Young and old leaves were approximately 2 months of age and 4 months of age, respectively. The young leaves contained more polyphenols and flavonoids than the old leaves. Three phenolic compounds (i.e., chlorogenic acid, p-coumaric acid, and rutin) were detected by HPLC. Antioxidant activity was measured using 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical, and superoxide anion radical scavenging assays. The reducing power of aronia leaf extracts increased in a concentration-dependent manner (0~100 μg/mL). The antioxidant activity of the 80% ethanol extract was greater than that of distilled water extract. The high phenolic compound content indicated that these compounds contribute to antioxidant activity. The overall results indicate that aronia leaves contain bioactive compounds, and that younger aronia leaves may be more favorable for extracting antioxidative ingredients because they contain more polyphenols. PMID:25320718

Aronia melanocarpa Treatment and Antioxidant Status in Selected Tissues in Wistar Rats

PubMed Central

Krośniak, Mirosław; Sanocka, Ilona; Bartoń, Henryk; Hebda, Tomasz; Francik, Sławomir

2014-01-01

Aronia juice is considered to be a source of compounds with high antioxidative potential. We conducted a study on the impact of compounds in the Aronia juice on oxidative stress in plasma and brain tissues. The influence of Aronia juice on oxidative stress parameters was tested with the use of a model with a high content of fructose and nonsaturated fats. Therefore, the activity of enzymatic (catalase, CAT, and paraoxonase, PON) and nonenzymatic (thiol groups, SH, and protein carbonyl groups, PCG) oxidative stress markers, which indicate changes in the carbohydrate and protein profiles, was marked in brain tissue homogenates. Adding Aronia caused statistically significant increase in the CAT activity in plasma in all tested diets, while the PON activity showed a statistically significant increase only in case of high fat diet. In animals fed with Aronia juice supplemented with carbohydrates or fat, statistically significant increase in the PON activity and the decrease in the CAT activity in brain tissue were observed. In case of the high fat diet, an increase in the number of SH groups and a decrease in the number of PCG groups in brain tissue were observed. PMID:25057488

Aronia melanocarpa treatment and antioxidant status in selected tissues in Wistar rats.

PubMed

Francik, Renata; Krośniak, Mirosław; Sanocka, Ilona; Bartoń, Henryk; Hebda, Tomasz; Francik, Sławomir

2014-01-01

Aronia juice is considered to be a source of compounds with high antioxidative potential. We conducted a study on the impact of compounds in the Aronia juice on oxidative stress in plasma and brain tissues. The influence of Aronia juice on oxidative stress parameters was tested with the use of a model with a high content of fructose and nonsaturated fats. Therefore, the activity of enzymatic (catalase, CAT, and paraoxonase, PON) and nonenzymatic (thiol groups, SH, and protein carbonyl groups, PCG) oxidative stress markers, which indicate changes in the carbohydrate and protein profiles, was marked in brain tissue homogenates. Adding Aronia caused statistically significant increase in the CAT activity in plasma in all tested diets, while the PON activity showed a statistically significant increase only in case of high fat diet. In animals fed with Aronia juice supplemented with carbohydrates or fat, statistically significant increase in the PON activity and the decrease in the CAT activity in brain tissue were observed. In case of the high fat diet, an increase in the number of SH groups and a decrease in the number of PCG groups in brain tissue were observed.

Improved stability of chokeberry juice anthocyanins by β-cyclodextrin addition and refrigeration.

PubMed

Howard, Luke R; Brownmiller, Cindi; Prior, Ronald L; Mauromoustakos, Andy

2013-01-23

Chokeberry anthocyanins are susceptible to degradation during processing and storage of processed products. This study determined the effects of three pH levels (2.8, 3.2, and 3.6) and four β-cyclodextrin (BCD) concentrations (0, 0.5, 1, and 3%) alone and in combination on the stability of chokeberry juice anthocyanins before and after pasteurization and over 8 months of storage at 4 and 25 °C. Lowering the pH from 3.6 to 2.8 in the absence of BCD provided marginal protection against anthocyanin losses during processing and storage. Addition of 3% BCD at the natural chokeberry pH of 3.6 resulted in excellent protection of anthocyanins, with 81 and 95% retentions after 8 months of storage at 25 and 4 °C, respectively. The protective effect of BCD was lessened with concentrations <3% and reduction in pH, indicating changes in anthocyanin structure play an important role in BCD stabilization of anthocyanins.

Fruit Wines Inhibitory Activity Against α-Glucosidase.

PubMed

Cakar, Uros; Grozdanic, Nada; Petrovic, Aleksandar; Pejin, Boris; Nastasijevic, Branislav; Markovic, Bojan; Dordevic, Brizita

2017-01-01

Fruit wines are well known for their profound health-promoting properties including both enzyme activations and inhibitions. They may act preventive in regard to diabetes melitus and other chronic diseases. Potential α-glucosidase inhibitory activity of fruit wines made from blueberry, black chokeberry, blackberry, raspberry and sour cherry was the subject of this study. In order to increase the alcohol content due to enriched extraction of total phenolics, sugar was added in the fruit pomace of the half of the examined fruit wine samples. Compared with acarbose used as a positive control (IC50 = 73.78 µg/mL), all fruit wine samples exhibited higher α-glucosidase inhibitory activity. Indeed, blueberry wine samples stood out, both prepared with IC50 = 24.14 µg/mL, lyophilised extract yield 3.23% and without IC50 = 46.39 µg/mL, lyophilised extract yield 2.89% and with addition of sugar before fermentation. Chlorogenic acid predominantly contributed to α-glucosidase inhibitory activity of the blueberry, black chokeberry and sour cherry wine samples. However, ellagic acid, a potent α-glucosidase inhibitor possessing a planar structure, only slightly affected the activity of the blueberry wine samples, due to the lower concentration. In addition to this, molecular docking study of chlorogenic acid pointed out the importance of binding energy (-8.5 kcal/mol) for the inhibition of the enzyme. In summary, fruit wines made from blueberry should be primarily taken into consideration as a medicinal food targeting diabetes mellitus type 2 in the early stage, if additional studies would confirm their therapeutic potential for the control of postprandial hyperglycemia. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Design, formulation and sensory evaluation of a polyphenol-rich food placebo: an example of aronia juice for food intervention studies.

PubMed

Kardum, Nevena; Konic Ristic, Aleksandra; Zec, Manja; Kojadinovic, Milica; Petrovic-Oggiano, Gordana; Zekovic, Milica; Kroon, Paul A; Glibetić, Maria

2017-09-01

Products suitable for use as controls in food interventions designed to demonstrate the role of minor components are largely lacking. In the present study, we aimed to develop a formulation to be used as a placebo in a clinical trial designed to assess the effects of aronia juice polyphenols on platelet function. Three formulations with the same nutrient composition as aronia juice were prepared by mixing various nutrients, artificial colours and flavours with water. The similarity of formulations to aronia juice in terms of taste, colour, smell and texture was assessed by six food panellists. The final placebo was tested for its impact on platelet function, biochemical and anthropometric parameters in a 4-week long study. No significant changes in platelet function, or in several cardiovascular and safety markers were recorded. Formulation suitable for use as a placebo for dietary intervention studies using aronia juice has been developed and demonstrated to be well tolerated in humans.

Characterisation of Aronia powders obtained by different drying processes.

PubMed

Horszwald, Anna; Julien, Heritier; Andlauer, Wilfried

2013-12-01

Nowadays, food industry is facing challenges connected with the preservation of the highest possible quality of fruit products obtained after processing. Attention has been drawn to Aronia fruits due to numerous health promoting properties of their products. However, processing of Aronia, like other berries, leads to difficulties that stem from the preparation process, as well as changes in the composition of bioactive compounds. Consequently, in this study, Aronia commercial juice was subjected to different drying techniques: spray drying, freeze drying and vacuum drying with the temperature range of 40-80 °C. All powders obtained had a high content of total polyphenols. Powders gained by spray drying had the highest values which corresponded to a high content of total flavonoids, total monomeric anthocyanins, cyaniding-3-glucoside and total proanthocyanidins. Analysis of the results exhibited a correlation between selected bioactive compounds and their antioxidant capacity. In conclusion, drying techniques have an impact on selected quality parameters, and different drying techniques cause changes in the content of bioactives analysed. Spray drying can be recommended for preservation of bioactives in Aronia products. Powder quality depends mainly on the process applied and parameters chosen. Therefore, Aronia powders production should be adapted to the requirements and design of the final product. Copyright © 2013 Elsevier Ltd. All rights reserved.

In vivo hemodynamic and electrocardiographic changes following Crataegus aronia syn. Azarolus L administration to normotensive Wistar rats.

PubMed

Shatoor, Abdullah S

2013-02-01

To evaluate the effects of the whole plant aqueous extract of Crataegus aronia (C. aronia) syn. Azarolus (L) on the hemodynamic and electrocardiographic intervals in albino rats. This study was carried out in 2 stages at the Research Laboratory, Physiology Department, Medical College of King Khalid University, Abha, Kingdom of Saudi Arabia between February and June 2012. First, the effects of C. aronia syn. Azarolus (L) on the hemodynamics and electrocardiograph in 54 Wistar male rats were assessed, then the mechanisms underlying the hemodynamic and electrocardiographic changes observed in the first stage were evaluated in 48 rats of the same species. The C. aronia administered at escalating doses (0.05-20 microgram/kg) produced a dose-time-dependent decrease in heart rate (HR) and mean arterial pressure (MAP). Higher doses (15 and 20 microgram/kg) produced the most significant reduction in both HR and MAP, and induced sinus node suppression and progressive atrio-ventricular blockade. The underlying mechanism of the induced bradyarrhythmia appeared to be due to the direct stimulation of the muscarinic receptor M2 and possible blockade of beta-receptors, while the hypotension was caused by enhanced nitric oxide release. No significant alterations in the electrocardiogram (ECG) components were observed. The administration of the C. aronia syn. Azarolus extract induced bradyarrhythmia and hypotension, without alteration in the ECG components.

Bioavailability of anthocyanins and colonic polyphenol metabolites following consumption of aronia berry extract.

PubMed

Xie, Liyang; Lee, Sang Gil; Vance, Terrence M; Wang, Ying; Kim, Bohkyung; Lee, Ji-Young; Chun, Ock K; Bolling, Bradley W

2016-11-15

A single-dose pharmacokinetic trial was conducted in 6 adults to evaluate the bioavailability of anthocyanins and colonic polyphenol metabolites after consumption of 500mg aronia berry extract. UHPLC-MS methods were developed to quantitate aronia berry polyphenols and their metabolites in plasma and urine. While anthocyanins were bioavailable, microbial phenolic catabolites increased ∼10-fold more than anthocyanins in plasma and urine. Among the anthocyanins, cyanidin-3-O-galactoside was rapidly metabolized to peonidin-3-O-galactoside. Aronia polyphenols were absorbed and extensively metabolized with tmax of anthocyanins and other polyphenol catabolites from 1.0h to 6.33h in plasma and urine. Despite significant inter-individual variation in pharmacokinetic parameters, concentrations of polyphenol metabolites in plasma and urine at 24h were positively correlated with total AUC in plasma and urine (r=0.93, and r=0.98, respectively). This suggests that fasting blood and urine collections could be used to estimate polyphenol bioavailability and metabolism after aronia polyphenol consumption. Copyright © 2016 Elsevier Ltd. All rights reserved.

In vivo influence of extract from Aronia melanocarpa on the erythrocyte membranes in patients with hypercholesterolemia.

PubMed

Duchnowicz, Piotr; Nowicka, Agmieszka; Koter-Michalak, Maria; Broncel, Marlena

2012-09-01

Hypercholesterolemia increases cholesterol concentration in erythrocyte membranes, which results in decrease of membrane fluidity and decreases the deformability of red blood cells. The fruits of Arona melanocarpa contains many of polyphenols and other compounds that have beneficial health effects. The aim of the study was to estimate the influence of 2-month supplementation of extract from Aronia melanocarpa (100 mg Aronox, three times per day) on cholesterol concentration, lipid peroxidation, membrane fluidity, level of thiol groups and activity of ATPase in erythrocytes from patients with hypercholesterolemia. The study involved 25 patients with hypercholesterolemia without pharmacological treatment and 20 healthy individuals as a control group. Blood samples were collected before, and after 1 and 2 months of Aronia administration. The 2-month Aronia supplementation resulted in a decrease of cholesterol concentration (by 22%) and a decrease of lipid peroxidation (by 40%), and an increase of membrane fluidity. No statistically significant increase of the concentration of thiol groups and of ATPase activity were observed. Our study shows that supplementation of extract from Aronia melanocarpa has a beneficial effect on rheological properties of erythrocytes.

Herbal-drug interaction induced rhabdomyolysis in a liposarcoma patient receiving trabectedin

PubMed Central

2013-01-01

Background Rhabdomyolysis is an uncommon side effect of trabectedin which is used for the second line therapy of metastatic sarcoma after anthracycline and ifosfamide failure. This side effect may be due to pharmacokinetic interactions caused by shared mechanisms of metabolism involving the cytochrome P450 (CYP) system in the liver. Here, for the first time in literature, we describe the unexpected onset of heavy toxicity, including rhabdomyolysis, after the fourth course of trabectedin in a patient with retroperitoneal liposarcoma who at the same time was taking an alternative herbal medicine suspected of triggering this adverse event. Case presentation This is the case of a 56 year old Caucasian man affected by a relapsed de-differentiated liposarcoma who, after the fourth cycle of second-line chemotherapy with trabectedin, complained of sudden weakness, difficulty walking and diffuse muscle pain necessitating complete bed rest. Upon admission to our ward the patient showed grade (G) 4 pancytopenia and a marked increase in liver lytic enzymes, serum levels of myoglobin, creatine phosphokinase (CPK) and lactate dehydrogenase. No cardiac or kidney function injuries were present. Based on these clinical and laboratory features, our conclusive diagnosis was of rhabdomyolysis induced by trabectedin. The patient did not report any trauma or muscular overexertion and no co-morbidities were present. He had not received any drugs during treatment with trabectedin, but upon further questioning the patient informed us he had been taking a folk medicine preparation of chokeberry (Aronia melanocarpa) daily during the last course of trabectedin and in the 2 subsequent weeks. One week after hospitalization and cessation of intake of chokeberry extract, CPK and other markers of myolysis slowly returned to standard range, and the patient noted a progressive recovery of muscle strength. The patient was discharged on day 14 when a blood transfusion and parenteral hydration gradually lowered general toxicity. Progressive mobilization of the patient was obtained as well as a complete normalization of the laboratory findings. Conclusions The level of evidence of drug interaction leading to the adverse event observed in our patient was 2 (probable). Thus our case underlines the importance of understanding rare treatment-related toxicities such as trabectedin-induced rhabdomyolysis and the possible role of the drug-drug interactions in the pathogenesis of this rare side effect. Furthermore, this report draws attention to a potential problem of particular concern, that of nutritional supplements and complementary and alternative drug interactions. These are not widely recognized and can cause treatment failure. PMID:23899130

In vivo influence of extract from Aronia melanocarpa on the erythrocyte membranes in patients with hypercholesterolemia

PubMed Central

Duchnowicz, Piotr; Nowicka, Agnieszka; Koter-Michalak, Maria; Broncel, Marlena

2012-01-01

Summary Background Hypercholesterolemia increases cholesterol concentration in erythrocyte membranes, which results in decrease of membrane fluidity and decreases the deformability of red blood cells. The fruits of Arona melanocarpa contains many of polyphenols and other compounds that have beneficial health effects. Material/Methods The aim of the study was to estimate the influence of 2-month supplementation of extract from Aronia melanocarpa (100 mg Aronox, three times per day) on cholesterol concentration, lipid peroxidation, membrane fluidity, level of thiol groups and activity of ATPase in erythrocytes from patients with hypercholesterolemia. The study involved 25 patients with hypercholesterolemia without pharmacological treatment and 20 healthy individuals as a control group. Blood samples were collected before, and after 1 and 2 months of Aronia administration. Results The 2-month Aronia supplementation resulted in a decrease of cholesterol concentration (by 22%) and a decrease of lipid peroxidation (by 40%), and an increase of membrane fluidity. No statistically significant increase of the concentration of thiol groups and of ATPase activity were observed. Conclusions Our study shows that supplementation of extract from Aronia melanocarpa has a beneficial effect on rheological properties of erythrocytes. PMID:22936193

Effect of Hawthorn (Crataegus aronia syn. Azarolus (L)) on platelet function in albino Wistar rats.

PubMed

Shatoor, Abdullah S; Soliman, Hesham; Al-Hashem, Fahaid; Gamal, Basiouny El-; Othman, Adel; El-Menshawy, Nadia

2012-07-01

This study was designed to investigate the possible antiplatelet effect of aqueous whole-plant C. aronia syn: Azarolus (L) extract using Wistar albino rats as a model. Forty-two male albino Wistar rats weighing 200 to 250 g were divided into seven groups with six rats in each group. Group 1 served as the control and received equal volumes of distilled water. Groups 2-6 served as the experimental groups and were given C. aronia extract at doses of 100, 200, 500, 1,000, and 2,000 mg/kg, while group 7 served as a positive control and was given aspirin (25mg/kg). All the doses were administered orally once a day and the treatment was continued for seven days. In all groups, at the end of the experimental procedure, blood samples were obtained for platelet function measurements, including PFA-100, thromboxane B2 levels, platelet count, and haematocrit. The bleeding time was determined using a modified tail cutting method described previously. The aqueous C. aronia syn. Azarolus (L) extract significantly altered the bleeding time and the closure time, as determined by the PFA-100 and thromboxane B2 levels, suggesting significant platelet function inhibition. These effects were observed with C. aronia doses between 100 - 500 mg/kg, which yielded thromboxane B2 levels of 1,000 mg/kg, whereas the higher dose (2,000 mg/kg) produced opposite effects on these parameters. C. aronia syn. Azarolus (L) aqueous extract has antiplatelet effects in Wistar albino rats. Copyright © 2012 Elsevier Ltd. All rights reserved.

Dietary Anthocyanins against Obesity and Inflammation

PubMed Central

Lee, Yoon-Mi; Yoon, Young; Yoon, Haelim; Park, Hyun-Min; Song, Sooji; Yeum, Kyung-Jin

2017-01-01

Chronic low-grade inflammation plays a pivotal role in the pathogenesis of obesity, due to its associated chronic diseases such as type II diabetes, cardiovascular diseases, pulmonary diseases and cancer. Thus, targeting inflammation is an attractive strategy to counter the burden of obesity-induced health problems. Recently, food-derived bioactive compounds have been spotlighted as a regulator against various chronic diseases due to their low toxicity, as opposed to drugs that induce severe side effects. Here we describe the beneficial effects of dietary anthocyanins on obesity-induced metabolic disorders and inflammation. Red cabbage microgreen, blueberry, blackcurrant, mulberry, cherry, black elderberry, black soybean, chokeberry and jaboticaba peel contain a variety of anthocyanins including cyanidins, delphinidins, malvidins, pelargonidins, peonidins and petunidins, and have been reported to alter both metabolic markers and inflammatory markers in cells, animals, and humans. This review discusses the interplay between inflammation and obesity, and their subsequent regulation via the use of dietary anthocyanins, suggesting an alternative dietary strategy to ameliorate obesity and obesity associated chronic diseases. PMID:28974032

Dietary Anthocyanins against Obesity and Inflammation.

PubMed

Lee, Yoon-Mi; Yoon, Young; Yoon, Haelim; Park, Hyun-Min; Song, Sooji; Yeum, Kyung-Jin

2017-10-01

Chronic low-grade inflammation plays a pivotal role in the pathogenesis of obesity, due to its associated chronic diseases such as type II diabetes, cardiovascular diseases, pulmonary diseases and cancer. Thus, targeting inflammation is an attractive strategy to counter the burden of obesity-induced health problems. Recently, food-derived bioactive compounds have been spotlighted as a regulator against various chronic diseases due to their low toxicity, as opposed to drugs that induce severe side effects. Here we describe the beneficial effects of dietary anthocyanins on obesity-induced metabolic disorders and inflammation. Red cabbage microgreen, blueberry, blackcurrant, mulberry, cherry, black elderberry, black soybean, chokeberry and jaboticaba peel contain a variety of anthocyanins including cyanidins, delphinidins, malvidins, pelargonidins, peonidins and petunidins, and have been reported to alter both metabolic markers and inflammatory markers in cells, animals, and humans. This review discusses the interplay between inflammation and obesity, and their subsequent regulation via the use of dietary anthocyanins, suggesting an alternative dietary strategy to ameliorate obesity and obesity associated chronic diseases.

The Influence of the Osmotic Dehydration Process on Physicochemical Properties of Osmotic Solution.

PubMed

Lech, Krzysztof; Michalska, Anna; Wojdyło, Aneta; Nowicka, Paulina; Figiel, Adam

2017-12-16

The osmotic dehydration (OD) process consists of the removal of water from a material during which the solids from the osmotic solution are transported to the material by osmosis. This process is commonly performed in sucrose and salt solutions. Taking into account that a relatively high consumption of those substances might have a negative effect on human health, attempts have been made to search for alternatives that can be used for osmotic dehydration. One of these is an application of chokeberry juice with proven beneficial properties to human health. This study aimed to evaluate the physicochemical properties of the OD solution (chokeberry juice concentrate) before and after the osmotic dehydration of carrot and zucchini. The total polyphenolics content, antioxidant capacity (ABTS, FRAP), dynamic viscosity, density, and water activity were examined in relation to the juice concentration used for the osmotic solution before and after the OD process. During the osmotic dehydration process, the concentration of the chokeberry juice decreased. Compounds with lower molecular weight and lower antioxidant capacity present in concentrated chokeberry juice had a stronger influence on the exchange of compounds during the OD process in carrot and zucchini. The water activity of the osmotic solution increased after the osmotic dehydration process. It was concluded that the osmotic solution after the OD process might be successfully re-used as a product with high quality for i.e. juice production.

Extract from Aronia melanocarpa fruits potentiates the inhibition of platelet aggregation in the presence of endothelial cells

PubMed Central

Luzak, Boguslawa; Golanski, Jacek; Rozalski, Marek; Krajewska, Urszula; Olas, Beata

2010-01-01

Introduction Some polyphenolic compounds extracted from Aronia melanocarpa fruits (AM) have been reported to be cardioprotective agents. In this study we evaluated the ability of AM extract to increase the efficacy of human umbilical vein endothelial cells (HUVECs) to inhibit platelet functions in vitro. Material and methods This study encompasses two models of monitoring platelet reactivity: optical aggregation and platelet degranulation (monitored as the surface CD62P expression) in PRP upon the stimulation with ADP. Results We observed that only at low concentrations (5 µg/ml) did AM extract significantly improve antiplatelet action of HUVECs towards ADP-activated platelets in the aggregation test. Conclusions It is concluded that the potentiating effect of AM extract on the endothelial cell-mediated inhibition of platelet aggregation clearly depends on the used concentrations of Aronia-derived active compounds. Therefore, despite these encouraging preliminary outcomes on the beneficial effects of AM extract polyphenols, more profound dose-effect studies should certainly be considered before the implementation of Aronia-originating compounds in antiplatelet therapy and the prevention of cardiovascular diseases. PMID:22371737

Protective effect of Aronia melanocarpa fruit juice in a model of cisplatin-induced cytotoxicity in vitro.

PubMed

Valcheva-Kuzmanova, Stefka V; Beronova, Anna B; Momekov, Georgi Tz

2013-01-01

The aim of the present study was to investigate the protective potential of Aronia melanocarpa fruit juice in a model of cisplatin-induced cytotoxicity in the human embryonal kidney cell line HEK293T. The cellular viability was assessed using the MTT-dye reduction assay based on the reduction of the yellow tetrazolium dye MTT to a violet formazan product via the mitochondrial succinate dehydrogenase in viable cells. Cisplatin was applied in various concentrations either alone or after a 24-hour pretreatment of the cells with Aronia melanocarpa fruit juice at 0.1 and 0.05 mg/ml. The half maximal inhibitory concentrations (IC50 values) were derived from the concentration-response curves to cisplatin. Applied alone, the anticancer drug caused a prominent decrease of cellular viability with IC50 8.3 +/- 1.1 microM. The juice proved to significantly ameliorate the in vitro cytotoxicity of the platinum drug, in a concentration-dependent manner. The pretreatment of the cells with Aronia melanocarpa fruit juice resulted in a significant increase (p < 0.001) of IC50 for cisplatin to 25.1 +/- 2.7 microM (at 0.05 mg/ml) and 34.4 +/- 3.4 microM (at 0.1 mg/ml), respectively. The protective effect of Aronia melanocarpa fruit juice observed in this study is most probably due to its well appreciated antioxidant activity as oxidative stress plays a central role in the toxic effects of cisplatin.

Evaluation of the hypocholesterolemic effect and phytochemical screening of the hydroethanolic extract of Crataegus aronia from Jordan.

PubMed

Al-Hallaq, Entisar K; Afifi, Fatma U; Abdalla, Shtaywy S

2012-01-01

Chemical screening of the leaves and flowers of Crataegus aronia resulted in the isolation of hyperoside, quercetin, rutin and beta-sitosterol for the first time from this plant. The effects of the hydroethanolic extract of C. aronia (CAHE) on hypercholesterolemic rats were investigated. The rats, treated orally for four weeks with 400 mg/kg/day CAHE, exhibited significant decreases in serum total cholesterol (TC) and low-density lipoprotein (LDL). The results were compared with those obtained after oral administration of atorvastatin (10 mg/kg/day). Furthermore, 10-week daily co-administration of a high cholesterol diet and CAHE (200 mg/kg/day) prevented the increase in TC and LDL. These observations indicate that CAHE has a hypocholesterolemic effect.

Effects of Aronia melanocarpa constituents on biofilm formation of Escherichia coli and Bacillus cereus.

PubMed

Bräunlich, Marie; Økstad, Ole A; Slimestad, Rune; Wangensteen, Helle; Malterud, Karl E; Barsett, Hilde

2013-12-05

Many bacteria growing on surfaces form biofilms. Adaptive and genetic changes of the microorganisms in this structure make them resistant to antimicrobial agents. Biofilm-forming organisms on medical devices can pose serious threats to human health. Thus, there is a need for novel prevention and treatment strategies. This study aimed to evaluate the ability of Aronia melanocarpa extracts, subfractions and compounds to prevent biofilm formation and to inhibit bacterial growth of Escherichia coli and Bacillus cereus in vitro. It was found that several aronia substances possessed anti-biofilm activity, however, they were not toxic to the species screened. This non-toxic inhibition may confer a lower potential for resistance development compared to conventional antimicrobials.

Profiling and Quantification of Regioisomeric Caffeoyl Glucoses in Berry Fruits.

PubMed

Patras, Maria A; Jaiswal, Rakesh; McDougall, Gordon J; Kuhnert, Nikolai

2018-02-07

On the basis of a recently developed tandem mass spectrometry-based hierarchical scheme for the identification of regioisomeric caffeoyl glucoses, selected berry fruits were profiled for their caffeoyl glucose ester content. Fresh edible berries profiled, including strawberries, raspberries, blueberries, blackberries, red currant, black currant, lingonberries, gooseberries, and juices of elderberries, goji berries, chokeberries, cranberries, açai berries, sea buckthorn berries, Montmorency sour cherries, and pomegranates, were investigated. 1-Caffeoyl glucose was found to be the predominant isomer in the majority of samples, with further profiling revealing the presence of additional hydroxycinnamoyl glucose esters and O-glycosides with p-coumaroyl, feruloyl, and sinapoyl substituents. A quantitative liquid chromatography-mass spectrometry-based method was developed and validated, and all caffeoyl glucose isomers were quantified for the first time in edible berries.

Effects of Aronia melanocarpa juice as part of the dietary regimen in patients with diabetes mellitus.

PubMed

Simeonov, Simeon B; Botushanov, Nikolai P; Karahanian, Eksapet B; Pavlova, Maria B; Husianitis, Haralambos K; Troev, Dimitar M

2002-01-01

The low calorie juice Aronia melanocarpa (sugar free, with artificial sweeteners) could be a valuable adjunct to the complex therapy of patients with diabetes mellitus. In this study no increased blood glucose levels were established 60 min. following ingestion of 200 ml Aronia juice. On the contrary, lower fasting blood glucose concentrations were measured in 16 patients with insulin dependent diabetes and in 25 patients with non-insulin dependent diabetes (25 women and 16 men, 3 to 62 years of age, median age 38.8 +/- 4.7) with duration of the disease from 1 month to 13 years. Serial blood glucose measurements showed: 14.23 +/- 1.32 mmol/l at baseline and 11.4 +/- 0.89 mmol/l blood glucose level after 60 min., the difference being statistically significant (p<0.05). The ingestion of 200 ml Aronia juice combined with a standard breakfast produced similar results (the basal concentration of glucose was 13.43 +/- 1.12 mmol/l; it decreased to 11.94 +/- 1.02 mmol/l at 60 min., the difference did not reach statistical significance. The daily intake of 200 ml Aronia juice over a period of 3 months was effective in lowering fasting blood glucose levels from 13.28 +/- 4.55 mmol/l to 9.10 +/- 3.05 mmol/l (p<0.001) in 21 patients with non-insulin dependent diabetes--13 women and 8 men aged from 42 to 62 (median age 53.6 +/- 3.65) with disease duration from 6 to 17 years. Aronia had a beneficial effect on HbA1c, total cholesterol and lipid levels. They dropped from 9.39 +/- 2.16% to 7.49 +/- 1.33% (p<0.001), from 6.45 +/- 1.59 mmol/l to 5.05 +/- 0.96 mmol/l (p<0.001) and from 2.92 +/- 2.15 mmol/l to 1.7 +/- 1.07 mmol/l (p<0.001), respectively. Results were compared with those obtained in 23 patients with non-insulin dependent diabetes (15 women and 8 men aged from 48 to 67 years, median age 54.9 +/- 3.34) with disease duration from 6 to 17 years. The above mentioned parameters remained unchanged in these patients. Accumulated data illustrated the hypoglycemic potential of Aronia juice. The precise mechanism of its action is unknown but its beneficial effects and good taste make it a valuable adjunct to the dietary treatment of patients with diabetes mellitus.

Characterizing and improving the sensory and hedonic responses to polyphenol-rich aronia berry juice.

PubMed

Duffy, Valerie B; Rawal, Shristi; Park, Jeeha; Brand, Mark H; Sharafi, Mastaneh; Bolling, Bradley W

2016-12-01

Interest in nutrient-rich berry juices is growing, but their high polyphenol levels render them sensorily unappealing. Fifty adults, who were assessed for sensory phenotype and dietary behaviors, provided sensory and palatability ratings of juices from 'Viking' aronia berries for each of seven harvest weeks. By peak harvest, juice preference increased two-fold, averaging neither like/dislike. This hedonic shift was associated with: increases in juice sugars paralleling increases in perceived sweetness (maximum = weak); reductions in percent acidity paralleling reductions in sourness (minimum = moderate), astringency (minimum = to just above weak) and bitterness (minimum = just below weak). About 25% of adults liked the aronia juice, including adults who also liked an aqueous citric acid solution (average rating = moderately sour) or those who reported adventurous eating behaviors. Bitter taste phenotype, measured by propylthiouracil or quinine bitterness, failed to explain significant variation in juice sensation or preference. We also collected sensory and preference ratings from juice collected at peak harvest blended with sugar and/or sweet olfactory flavoring (10 ppm ethyl butyrate). Increasing juice sweetness by adding 5% sucrose decreased sourness and improved preference from weak dislike to weak like. Adding sweet olfactory flavoring decreased juice sourness without changing preference. Adding sweet flavoring and 3% sucrose resulted in reduction of sourness and improvements in preference ratings comparable to 5% added sucrose. Neither added sugar nor flavoring blocked juice astringency. In summary, these findings suggest that aronia juice, even from berries picked at peak harvest, appealed to only a few adults (sour likers or adventurous eaters). Although enhanced sweetness, with added sugar and sweet olfactory flavoring, improved aronia juice preference, broader sensory approaches are required to blunt astringency for greater consumer appeal. Copyright © 2016 Elsevier Ltd. All rights reserved.

Characterizing and improving the sensory and hedonic responses to polyphenol-rich aronia berry juice

PubMed Central

Duffy, Valerie B.; Rawal, Shristi; Park, Jeeha; Brand, Mark H.; Sharafi, Mastaneh; Bolling, Bradley W.

2017-01-01

Interest in nutrient-rich berry juices is growing, but their high polyphenol levels render them sensorily unappealing. Fifty adults, who were assessed for sensory phenotype and dietary behaviors, provided sensory and palatability ratings of juices from ‘Viking’ aronia berries for each of seven harvest weeks. By peak harvest, juice preference increased two-fold, averaging neither like/dislike. This hedonic shift was associated with: increases in juice sugars paralleling increases in perceived sweetness (maximum = weak); reductions in percent acidity paralleling reductions in sourness (minimum = moderate), astringency (minimum = to just above weak) and bitterness (minimum = just below weak). About 25% of adults liked the aronia juice, including adults who also liked an aqueous citric acid solution (average rating = moderately sour) or those who reported adventurous eating behaviors. Bitter taste phenotype, measured by propylthiouracil or quinine bitterness, failed to explain significant variation in juice sensation or preference. We also collected sensory and preference ratings from juice collected at peak harvest blended with sugar and/or sweet olfactory flavoring (10 ppm ethyl butyrate). Increasing juice sweetness by adding 5% sucrose decreased sourness and improved preference from weak dislike to weak like. Adding sweet olfactory flavoring decreased juice sourness without changing preference. Adding sweet flavoring and 3% sucrose resulted in reduction of sourness and improvements in preference ratings comparable to 5% added sucrose. Neither added sugar nor flavoring blocked juice astringency. In summary, these findings suggest that aronia juice, even from berries picked at peak harvest, appealed to only a few adults (sour likers or adventurous eaters). Although enhanced sweetness, with added sugar and sweet olfactory flavoring, improved aronia juice preference, broader sensory approaches are required to blunt astringency for greater consumer appeal. PMID:27457970

In vitro inhibition of cytochrome P450 3A4 by Aronia melanocarpa constituents.

PubMed

Bräunlich, Marie; Christensen, Hege; Johannesen, Siri; Slimestad, Rune; Wangensteen, Helle; Malterud, Karl E; Barsett, Hilde

2013-01-01

Extracts, subfractions, isolated anthocyanins and procyanidins, and two phenolic acids from aronia [Aronia melanocarpa] were investigated for their CYP3A4 inhibitory effects, using midazolam as the probe substrate and recombinant insect cell microsomes expressing CYP3A4 as the enzyme source. Procyanidin B5 was a considerably stronger CYP3A4 inhibitor in vitro than the isomeric procyanidin B2 and comparable to bergamottin, a known CYP3A4 inhibitor from grapefruit juice. The inhibitory activity of proanthocyanidin-containing fractions was correlated to the degree of polymerization. Among the anthocyanins, cyanidin 3-arabinoside showed stronger CYP3A4 inhibition than cyanidin 3-galactoside and cyanidin 3-glucoside. Thus, the ability to inhibit CYP3A4 in vitro seems to be influenced by the sugar unit linked to the anthocyanidin. Georg Thieme Verlag KG Stuttgart · New York.

Effect of Bioactive Compound of Aronia melanocarpa on Cardiovascular System in Experimental Hypertension.

PubMed

Cebova, Martina; Klimentova, Jana; Janega, Pavol; Pechanova, Olga

2017-01-01

Aronia melanocarpa has attracted scientific interest due to its dense contents of different polyphenols. We aimed to analyse effects of Aronia melanocarpa (AME) extract on blood pressure (BP), lipid peroxidation, cytokine level, total NOS activity in the left ventricle (LV), and aorta of L-NAME-induced hypertensive rats. 12-week-old male WKY rats were assigned to the control group and groups treated with AME extract (57.90 mg/kg/day), L-NAME (40 mg/kg/day), or combination of L-NAME (40 mg/kg/day) and AME (57.90 mg/kg/day) in tap water for 3 weeks. NOS activity, eNOS protein expression, and conjugated diene (CD) concentration were determined in the LV and aorta. After 3 weeks of L-NAME treatment, BP was increased by 28% and concomitant treatment with AME reduced it by 21%. NOS activity of the LV and aorta in the L-NAME group was decreased by about 40%, while AME increased it almost on the control level. AME-induced eNOS upregulation may contribute to increase NOS activity. Moreover, AME decreased CD concentration in the LV and aorta and TNF- α and IL-6 production in the plasma were increased by L-NAME treatment. In conclusion, our results showed that active substances of Aronia melanocarpa may have a positive effect on blood pressure, NOS activity, and proinflammatory processes in L-NAME-induced hypertension.

Enhancement of Cognitive Functions by Aronia melanocarpa Elliot Through an Intermittent Ultrasonication Extraction Process.

PubMed

Kim, Nam Young; Lee, Hyeon Yong

2016-03-01

To increase the cognitive enhancement provided by Aronia melanocarpa Elliot (Aronia), Aronia was extracted using 70% ethanol solvent and six cycles of intermittent ultrasonication at 120 KHz for 50 min, followed by a rest for 10 min (UE), and was also extracted using 70% ethanol for 24 h at 80°C (EE) as a control process. In both in vivo water maze and passive avoidance tests, the UE showed better performance enhancement than the EE: in the water maze, mice treated with EE and UE showed escape latencies of 62.6 s and 54.3 s, respectively; for passive avoidance, they showed retention times of 45.9 s and 38.9 s, respectively. UE downregulated the expression level of acetylcholinesterase genes to 1.46 times compared with 1.72 for EE. However, there were no significant histological differences in the hippocampus between the mice fed with EE and those fed UE. Additionally, the UE was confirmed to have a greater antioxidant effect, 0.728 versus 0.561 for EE. Comparison of the high-performance liquid chromatography chromatograms of the extracts demonstrates that the intermittent ultrasonication process may improve the cognitive activities of Aronia by eluting higher amounts of cyanidin-3-galactoside (C3G). This work is the first to report that the crude extract from the intermittent ultrasonication process provided better cognitive enhancement than a single major bioactive substance, C3G itself, possibly through the synergistic effects of other anthocyanins present in the extract, such as delphine galactoside, cyanidin arabinoside, and cyanidin glucoside. We also believe that these findings may provide a reliable basis for developing natural plant drugs to compensate for the side effects of purified and/or chemically synthesized single-component drugs rather than to compete with them.

Effect of Bioactive Compound of Aronia melanocarpa on Cardiovascular System in Experimental Hypertension

PubMed Central

Klimentova, Jana; Janega, Pavol

2017-01-01

Aronia melanocarpa has attracted scientific interest due to its dense contents of different polyphenols. We aimed to analyse effects of Aronia melanocarpa (AME) extract on blood pressure (BP), lipid peroxidation, cytokine level, total NOS activity in the left ventricle (LV), and aorta of L-NAME-induced hypertensive rats. 12-week-old male WKY rats were assigned to the control group and groups treated with AME extract (57.90 mg/kg/day), L-NAME (40 mg/kg/day), or combination of L-NAME (40 mg/kg/day) and AME (57.90 mg/kg/day) in tap water for 3 weeks. NOS activity, eNOS protein expression, and conjugated diene (CD) concentration were determined in the LV and aorta. After 3 weeks of L-NAME treatment, BP was increased by 28% and concomitant treatment with AME reduced it by 21%. NOS activity of the LV and aorta in the L-NAME group was decreased by about 40%, while AME increased it almost on the control level. AME-induced eNOS upregulation may contribute to increase NOS activity. Moreover, AME decreased CD concentration in the LV and aorta and TNF-α and IL-6 production in the plasma were increased by L-NAME treatment. In conclusion, our results showed that active substances of Aronia melanocarpa may have a positive effect on blood pressure, NOS activity, and proinflammatory processes in L-NAME-induced hypertension. PMID:29333212

Memory effects of Aronia melanocarpa fruit juice in a passive avoidance test in rats.

PubMed

Valcheva-Kuzmanova, Stefka V; Eftimov, Miroslav Tz; Tashev, Roman E; Belcheva, Iren P; Belcheva, Stiliana P

2014-01-01

To study the effect of Aronia melanocarpa fruit juice on memory in male Wistar rats. The juice was administered orally for 7, 14, 21 and 30 days at doses of 2.5 ml/kg, 5 ml/kg and 10 ml/kg. Memory was assessed in the one-way passive avoidance task (step through) which consisted of one training session and two retention tests (3 hours and 24 hours after training). The variables measured were the latency time to step into the dark compartment of the apparatus and the learning criterion (remaining in the illuminated compartment for at least 180 sec). Oral administration of Aronia melanocarpa fruit juice for 7 and 14 days resulted in a dose-dependent tendency to increase the latency time and the learning criterion compared to saline-treated controls but the effect failed to reach statistical significance. After 21 days of treatment, the juice dose-dependently prolonged the latency time at the retention tests, the effect being significant at doses of 5 ml/kg and 10 ml/kg. Applied for 30 days, the juice in all the tested doses increased significantly the latency time at the retention tests and the dose of 10 ml/kg significantly increased the percentage of rats reaching the learning criterion. These findings suggest that Aronia melanocarpa fruit juice could improve memory in rats. The effect is probably due to the polyphenolic ingredients of the juice which have been shown to be involved in learning and memory processes.

Gamma-irradiated dry fruits. An example of a wide variety of long-time dependent EPR spectra

NASA Astrophysics Data System (ADS)

Yordanov, Nicola D.; Pachova, Zdravka

2006-03-01

EPR spectra of dry, sugar containing fruits—raisins, sultanas, figs, dates, peaches, blue plums and chokeberry recorded before and after irradiation with gamma-rays, are reported. It is shown that weak singlet EPR line with 2.0031 ± 0.0005 can be recorded before irradiation of seeds, stones or skin of chokeberry, figs and raisins as well as flesh of blue plum, raisins and peaches. EPR signals of various shape are distinguished after irradiation in different parts of the fruits, as well as in randomly cut pieces of them: Seeds of raisins, chokeberry and figs give a singlet line. Stones from blue plums and peaches exhibit typical "cellulose-like" EPR signal consisting of an intense singlet line with g = 2.0033 ± 0.0005 and 2 week satellite lines situated ca. 30 G left and right to it. Stones of dates are the only sample in which "sugar-like" spectrum is recorded. Skin of raisins and figs exhibits "sugar-like" EPR spectrum whereas that of dates and chokeberry—a singlet line. Under the same experimental conditions skin of sultanas, peaches and blue plums are EPR silent. Flesh of raisins, sultanas, figs, dates and peaches exhibits "sugar-like" EPR spectrum, flesh of blue plums gives a singlet EPR line and that of chokeberry is EPR silent. As a result, randomly cut pieces of dry fruits suitable for EPR studies, containing various constituents, exhibit different in shape and intensity EPR spectra. Kinetic studies followed for 1 year on the time stability of all reported EPR signals indicate that intensity ratio between the simultaneously appearing EPR signals in particular fruit varies from 1:20 immediately after irradiation to 1:0.5 at the end of the period. These observations open a new possibility for identification of irradiated fruits - using the magnitude of the intensity ratio to find the approximate date of radiation processing in the first ca. 30-100 days.

Triterpene constituents from the seedling of Aronia melanocarpa.

PubMed

Yu, M; Li, X; Zhao, C-C; Xu, J; Zhang, P

2007-01-01

Two new triterpene saponins, 16-O-acetyl-21-O-angeloyltheasapogenol A 3-O-[beta-D-galactopyranosyl(1 --> 2)][beta-D-xylopyranosyl(1 --> 2)-alpha-L-arabinopyranosyl (1 --> 3)]-beta-D-glucopyranosiduronic acid (1) and 16,28-O-diacetyl-21-O-tigloyltheasapogenol A 3-O-[beta-D-galactopyranosyl(1 --> 2)][beta-D-xylopyranosyl(1 --> 2)-alpha-L-arabinopyranosyl (1 --> 3)]-beta-D-glucopyranosiduronic acid (2), together with four known triterpenenes, have been isolated from the dried seedling of Aronia melanocarpa, and their structures established by spectroscopic and chemical evidence.

Oxidative stability of the meat of broilers supplemented with rosemary leaves, rosehip fruits, chokeberry pomace, and entire nettle, and effects on performance and meat quality.

PubMed

Loetscher, Y; Kreuzer, M; Messikommer, R E

2013-11-01

Prevention of lipid oxidation needs special attention because a high proportion of fatty acids in broiler meat are unsaturated. A feeding experiment was conducted to evaluate the antioxidant effect of dietary addition of rosemary, chokeberry pomace, rosehip, or nettle in comparison with vitamin E. Male Ross PM3 broilers caged in groups of 6 (4 replicated cages per treatment) were fed a balanced diet supplemented with 25 g/kg of herbal additive, 200 IU of α-tocopheryl acetate/kg, or without supplementation from d 7 to 35. Intake, performance, and with the help of excreta samples, apparent fiber digestibility, ME content, and metabolizability of nitrogen and energy were recorded per cage. Feed was analyzed for total phenols and tocopherols. In each bird (n = 24 per treatment), carcass weight and relative organ weights were recorded, and skin and liver color were assessed. Abdominal fat was analyzed for induction time (h) of lipid oxidation (Rancimat). Breast meat was analyzed for total tocopherol content (mg/kg) and development of TBA reactive substances (TBARS; μg of MDA/kg) over 9 d of storage. Data were subjected to ANOVA considering treatment and, where applicable, storage time. Rosemary supplementation reduced oxidation (TBARS d 9: 201; induction time: 2.48) and elevated tocopherol content (5.72) of the meat compared with control (470, 1.87, and 3.53, respectively). Rosemary-treated birds had a slightly lower carcass weight and a reduced nitrogen and energy metabolizability. Rosehip addition numerically decreased TBARS (319) and enhanced carcass weight (1.71 kg) compared with rosemary-treated birds (1.54 kg). Only a trend in antioxidant activity could be ascribed to chokeberry pomace, although dietary phenolic content was highest. Nettle did not improve oxidative stability (TBARS: 506; induction time: 1.91), although tocopherol content was elevated (6.51). Nettle treatment strongly intensified skin yellowness (b* of 20.6) compared with the control treatment (10.8). Clearly, rosemary is the most suitable dietary antioxidant investigated in this study, yet chokeberry and rosehip also exhibited interesting properties.

DNA catabolites in triathletes: effects of supplementation with an aronia-citrus juice (polyphenols-rich juice).

PubMed

García-Flores, Libia Alejandra; Medina, Sonia; Cejuela-Anta, Roberto; Martínez-Sanz, José Miguel; Abellán, Ángel; Genieser, Hans-Gottfried; Ferreres, Federico; Gil-Izquierdo, Ángel

2016-04-01

In this study we analyzed whether our aronia-citrus juice (ACJ, the composition is based on a mixture of 95% citrus juice with 5% of Aronia melanocarpa juice), rich in polyphenols, and physical exercise had an effect on seven catabolites of DNA identified in plasma and on a urine isoprostane (8-iso-PGF2α). Sixteen elite triathletes on a controlled diet for triathlon training (45 days) were used in this clinical trial. Our results show a decrease in the 8-hydroxy-2'-deoxyguanosine concentration due to chronic physical exercise. The ACJ intake and physical exercise maintained the guanosine-3',5'-cyclic monophosphate plasmatic concentrations and decreased the concentration of 8-hydroxyguanine as well as urinary values of 8-iso-PGF2α. Finally, we observed a significant increase in the 8-nitroguanosine levels in triathletes after ACJ intake, compared to the placebo stage. It is concluded that the combination of the intake of ACJ, rich in polyphenolic compounds, with adequate training was able to influence the plasmatic and urinary values of oxidative stress biomarkers. This suggests a positive effect on the oxidative damage and potential associations with DNA repair mechanisms.

[Antiradical activity and resistance of flaxseed oil enriched with the antioxidants to oxidative changes].

PubMed

Guseva, D A; Prozorovskaia, N N; Rusina, I F; Ipatova, O M

2008-01-01

Using the chemiluminescence technique, the effective concentration of antioxidants (AO) and its reactivity towards peroxyl radicals (constant k7) have been measured for the oil "Ubicato-flax" (biological active supplement) and flaxseed oil, enriched with Aronia melanocarpa extract. Ubicato-flax exhibited the highest antiradical activity but it was less resistant to oxidative changes as compared with flaxseed oil + extract. Ubicato oil produced on basis of natural AO exhibited higher antiradical activity in comparison with Ubicato oil produced on basis of the chemical synthetic AO. Aronia extract added to flaxseed oil caused marked inhibition of the increase in the peroxide value. Ubicato-flax may serve as a source of AO for not more than 6 months.

Development and validation of an LC-MS/MS method for the determination of biogenic amines in wines and beers.

PubMed

Nalazek-Rudnicka, Katarzyna; Wasik, Andrzej

2017-01-01

Biogenic amines are group of organic, basic, nitrogenous compounds that naturally occur in plant, microorganism, and animal organisms. Biogenic amines are mainly produced through decarboxylation of amino acids. They are formed during manufacturing of some kind of food and beverages such as cheese, wine, or beer. Histamine, cadaverine, agmatine, tyramine, putrescine, and β -phenylethylamine are the most common biogenic amines found in wines and beers. This group of compounds can be toxic at high concentrations; therefore, their control is very important. Analysis of biogenic amines in alcoholic drinks (beers and wines) was carried out by HPLC-MS/MS after their derivatization with p -toluenesulfonyl chloride (tosyl chloride). The developed method has been applied for analysis of seventeen biogenic amines in twenty-eight samples of lager beers and in twelve samples of different homemade wines (white grape, red grape, strawberry, chokeberry, black currant, plum, apple, raspberry, and quince). The developed method is sensitive and repeatable for majority of the analytes. It is versatile and can be used for the determination of biogenic amines in various alcoholic beverages.

Antioxidant Activities of Selected Berries and Their Free, Esterified, and Insoluble-Bound Phenolic Acid Contents

PubMed Central

2018-01-01

To explore the potential of berries as natural sources of bioactive compounds, the quantities of free, esterified, and insoluble-bound phenolic acids in a number of berries were determined. In addition, the antioxidant activities of the berries were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, and Trolox equivalent antioxidant capacity assays, in addition to determination of their metal ion chelating activities. Furthermore, several phenolic compounds were detected using high-performance liquid chromatography. Of the 6 tested berries, black chokeberry and blackberry exhibited the strongest antioxidant activities, and the various berry samples were found to contain catechin, caffeic acid, p-coumaric acid, epicatechin, vanillic acid, quercitrin, resveratrol, morin, naringenin, and apigenin. Moreover, the antioxidant activities and total phenolic contents of the fractions containing insoluble-bound phenolic acids were higher than those containing the free and esterified phenolic acids. The results imply that the insoluble-bound fractions of these berries are important natural sources of antioxidants for the preparation of functional food ingredients and preventing diseases associated with oxidative stress. PMID:29662846

Anthocyanin- and proanthocyanidin-rich extracts of berries in food supplements--analysis with problems.

PubMed

Krenn, L; Steitz, M; Schlicht, C; Kurth, H; Gaedcke, F

2007-11-01

The fundamental nutritional benefit of fruit and vegetables in the prevention of degenerative diseases--especially in the light of the current "anti-aging wave"--has directed the attention of scientists and consumers to a variety of berry fruits and their constituents. Many of these fruits, e.g. blueberries, elderberries or cranberries, have a long tradition in European and North American folk medicine. Based on these experiences and due to the growing interest the number of food supplements on the market containing fruit powders, juice concentrates or extracts of these fruits has increased considerably. Advertising for these products mainly focusses on the phenolic compounds, especially the anthocyanins and proanthocyanidins and their preventive effects. Most of the preparations are combinations, e.g. of extracts of different fruits with vitamins and trace elements, etc. which are labelled in a way which does not allow a comparison of the products. Typically, information on the extraction solvent, the drug: extract ratio and the content of anthocyanins and proanthocyanidins is missing. Besides that, the analysis of these polyphenols causes additional problems. Whereas the quality control of herbal medicinal products is regulated in detail, no uniform requirements for food supplements are existing. A broad spectrum of methods is used for the assay of the constituents, leading to differing, incomparable results. In addition to that, the methods are quite interference-prone and consequently lead to over- or underestimation of the contents. This publication provides an overview of some selected berries (lingonberry, cranberry, black elderberry, black chokeberry, black currant, blueberry), their constituents and use. The analytical methods currently used for the identification and quantification of the polyphenols in these berries are described, including an evaluation of their advantages and disadvantages.

Aronia melanocarpa fruit extract exhibits anti-inflammatory activity in human aortic endothelial cells.

PubMed

Zapolska-Downar, D; Bryk, D; Małecki, M; Hajdukiewicz, K; Sitkiewicz, D

2012-08-01

Altered expression of cell adhesion molecules (CAMs) has been implicated in a variety of chronic inflammatory conditions, including atherosclerosis. Regulation of adhesion molecule expression by specific redox-sensitive mechanisms has been reported. Additionally, it has been observed that the extract of Aronia melanocarpa (A. Melanocarpa) fruits, rich in polyphenols, exhibits potent anti-oxidant properties and displays cardioprotective activity. Human aortic endothelial cells (HAECs) were pretreated with various concentrations (primarily 50 μg/mL) of Aronia Melanocarpa fruit extract prior to treatment with TNFα (10 ng/mL) for various periods of time. The surface protein and mRNA expression of ICAM-1 and VCAM-1 were determined using flow cytometry and real-time RT-PCR, respectively. Adhesion of peripheral blood mononuclear leucocytes (PBMLs) to TNFα-treated HAECs was evaluated by an adhesion assay. Activation of NF-κB was evaluated by measuring NF-κB p65 phosphorylation using flow cytometry. ROS production was determined by reduction in fluorescent 2',7'-dichlorofluorescein diacetate (DCFH-DA). Tested A. Melanocarpa extract significantly inhibited the expression of ICAM-1 and VCAM-1, attenuated the phosphorylation of NF-κB p65 and decreased intracellular ROS production in TNFα-treated HAECs. We conclude that A. Melanocarpa fruit extract exhibits anti-inflammatory effects in HAECs by inhibiting the expression of endothelial CAMs, activation of NF-κB and production of ROS.

Anxiolytic-like effect of Aronia melanocarpa fruit juice in rats.

PubMed

Valcheva-Kuzmanova, S; Zhelyazkova-Savova, M

2009-12-01

The main biologically active constituents of Aronia melanocarpa fruit juice (AMFJ) are polyphenolics, amongst them flavonoids, mainly anthocyanins. The aim of the present study was to investigate the effects of AMFJ (5 and 10 mL/kg) on anxiety using the social interaction test, on locomotor activity in the open field test and on working memory in the object recognition test in rats. AMFJ showed an anxiolytic-like effect which was demonstrated by a dose-dependent increase in the time of active social contacts between the test partners. The effects of both AMFJ doses were comparable to the effect of diazepam (1 mg/kg). AMFJ neither changed significantly horizontal and vertical locomotor activity, nor did it adversely affect working memory. Copyright 2009 Prous Science, S.A.U. or its licensors. All rights reserved.

The importance of monochromatic lights in the production of phenolic acids and flavonoids in shoot cultures of Aronia melanocarpa, Aronia arbutifolia and Aronia × prunifolia.

PubMed

Szopa, Agnieszka; Starzec, Anna; Ekiert, Halina

2018-02-01

Shoot cultures of Aronia melanocarpa, A. arbutifolia and A. × prunifolia were maintained on Murashige and Skoog medium with 1 mg/l each of BA and NAA under monochromatic lights (far-red, red, blue lights, UV-A-irradiation), in darkness, and under white light (control). HPLC-DAD analyses of 19 phenolic acids and 11 flavonoids in methanolic extracts from the shoots revealed in all of them the presence of three depsides (chlorogenic, neochlorogenic and rosmarinic acids), protocatechuic acid, four flavonoid glycosides (cynaroside, quercitrin, hyperoside and rutoside), and additionally, in A. arbutifolia, 3,4-dihydroxyphenylacetic acid. Depending on light quality, the total amounts of these metabolites increased 1.8-5.9 times, reaching maximum values under blue light: 527.40 and 144.61 mg 100 g -1 DW (A. melanocarpa), 543.27 and 85.82 mg 100 g -1 DW (A. arbutifolia) and 1615.18 and 220.65 mg 100 g -1 DW (A. × prunifolia), respectively. The maximum total amounts were 1.3-3.6 times higher than under white light. The quantities of individual metabolites changed from 1.2 to 11.0 times, with high amounts of neochlorogenic acid and quercitrin in A. melanocarpa (243.35 and 75.64 mg 100 g -1 DW), and of chlorogenic and rosmarinic acids and quercitrin in A. arbutifolia (236.52, 219.35 and 51.01 mg 100 g -1 DW). Extremely high amounts of depsides (418.83, 644.68, 548.86 mg 100 g -1 DW) and quercitrin (165.88 mg 100 g -1 DW) were produced in cultures of the hybrid - A. × prunifolia. The results are potentially useful for practical applications. This is the first report documented the importance of light quality on the production of phenolic acids and flavonoids in three aronia in vitro cultures. Copyright © 2018. Published by Elsevier B.V.

Aronia melanocarpa extract reduces blood pressure, serum endothelin, lipid, and oxidative stress marker levels in patients with metabolic syndrome.

PubMed

Broncel, Marlena; Kozirog, Marzena; Duchnowicz, Piotr; Koter-Michalak, Maria; Sikora, Joanna; Chojnowska-Jezierska, Julita

2010-01-01

Experimental studies have shown that anthocyanins may exert pleiotropic effects. The aim of the study was to determine the influence of Aronia melanocarpa extract on blood pressure and serum concentration of endothelin-1 (ET-1), lipids, glucose, uric acid, C-reactive protein (CRP), fibrinogen, the antioxidant enzymes catalase (CAT), superoxide dysmutase (SOD), and glutathione peroxidase (GSH-Px), and lipid peroxidation (thiobarbituric acid-reacting substrates, TBARS) in erythrocytes of patients with metabolic syndrome (MS). The study comprised 22 healthy volunteers and 25 patients with MS. Patients with MS were treated with aronia extract (3 x 100 mg/day) for two months. The above parameters were measured. After two months of therapy, statistically significant decreases were observed in SBP (143.40+/-7.87 vs. 131.83+/-12.24 mmHg, p<0.001), DBP (87.20+/-9.9 vs. 82.13+/-10.33 mmHg, p<0.05), ET-1 (2.44+/-0.51 vs. 1.74+/-0.42 pg/ml, p<0.001), TC (242.80+/-34.48 vs. 227.96+/-33.07 mg/dl, p<0.001), LDL-C (158.71+/-35.78 vs. 146.21+/-34.63 mg/dl, p<0.01), TG (215.92+/-63.61 vs. 187.58+/-90 mg/dl, p<0.05), TBARS (0.0712+/-0.0191 vs. 0.0362+/-0.0135 micromol/g-Hb, p<0.001), and CAT (261.30+/-59.78 vs. 213.34+/-47.36 U/mg-Hb) and significant increases in SOD (2380.63+/-419.91 vs. 3066.53+/-542.24 U/g-Hb, p<0.001), GSH-Px (12.60+/-5.97 vs. 19.18+/-9.09 U/g-Hb, p<0.01), and fibrinogen levels (249.20+/-27.17 vs. 276.67+/-57.41 mg/dl, p<0.05) compared with the baseline values. Aronia extract may be of benefit to patients with MS. This seems to result from the influence of anthocyanins and possibly other flavonoids on blood pressure, serum level of ET-1, lipids, and oxidative status (GSH-Px, SOD, TBARS).

The effects of Crataegus aronia var. dentata Browicz extract on biochemical indices and apoptosis in partially hepatectomized liver in rats.

PubMed

Keskin, Nazan; Mammadov, Ramazan; Ili, Pinar

2012-08-01

Crataegus species have been widely used in herbal medicine, especially for the hearth diseases. In the present study, the effect of Crataegus aronia var. dentata Browicz extract on partially hepatectomized rats was investigated with biochemical and TUNEL apoptosis assays. The extracts of the plant at the concentrations of 0.5 and 1 ml/100 g body weight/day were administered orally to the two experimental groups including partially hepatectomized rats for 42 days. At the end of the experimental period, animals were sacrificed, blood was collected for the assessment of serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltransferase (GGT), and the liver tissue was used for TUNEL assay. In biochemical assay, it was found a significant decrease in the levels of serum ALT and AST in the experimental groups. On the other hand, the plant extract did not cause any significant changes in the level of GGT in these groups. In apoptosis assay, TUNEL positive hepatocytes could not be detected in both experimental groups. The present findings can suggest that Crataegus aronia var. dentata Browicz extract can decrease the levels of serum ALT and AST and play a role in apoptosis of hepatocytes in the liver of partially hepatectomized rats. However, further studies are required to confirm the effects of the plant extract on hepatoprotection and apoptosis in the regenerating liver after partial hepatectomy in animal models.

The effects of Crataegus aronia var. dentata Browicz extract on biochemical indices and apoptosis in partially hepatectomized liver in rats

PubMed Central

Keskin, Nazan; Mammadov, Ramazan; Ili, Pinar

2012-01-01

Crataegus species have been widely used in herbal medicine, especially for the hearth diseases. In the present study, the effect of Crataegus aronia var. dentata Browicz extract on partially hepatectomized rats was investigated with biochemical and TUNEL apoptosis assays. The extracts of the plant at the concentrations of 0.5 and 1 ml/100 g body weight/day were administered orally to the two experimental groups including partially hepatectomized rats for 42 days. At the end of the experimental period, animals were sacrificed, blood was collected for the assessment of serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltransferase (GGT), and the liver tissue was used for TUNEL assay. In biochemical assay, it was found a significant decrease in the levels of serum ALT and AST in the experimental groups. On the other hand, the plant extract did not cause any significant changes in the level of GGT in these groups. In apoptosis assay, TUNEL positive hepatocytes could not be detected in both experimental groups. The present findings can suggest that Crataegus aronia var. dentata Browicz extract can decrease the levels of serum ALT and AST and play a role in apoptosis of hepatocytes in the liver of partially hepatectomized rats. However, further studies are required to confirm the effects of the plant extract on hepatoprotection and apoptosis in the regenerating liver after partial hepatectomy in animal models. PMID:22938545

Osmotic dehydration of Braeburn variety apples in the production of sustainable food products

NASA Astrophysics Data System (ADS)

Ciurzyńska, Agnieszka; Cichowska, Joanna; Kowalska, Hanna; Czajkowska, Kinga; Lenart, Andrzej

2018-01-01

The aim of this work was to investigate the effects of osmotic dehydration conditions on the properties of osmotically pre-treated dried apples. The scope of research included analysing the most important mass exchange coefficients, i.e. water loss, solid gain, reduced water content and water activity, as well as colour changes of the obtained dried product. In the study, apples were osmotically dehydrated in one of two 60% solutions: sucrose or sucrose with an addition of chokeberry juice concentrate, for 30 and 120 min, in temperatures of 40 and 60°C. Ultrasound was also used during the first 30 min of the dehydration process. After osmotic pre-treatment, apples were subjected to innovative convective drying with the puffing effect, and to freeze-drying. Temperature and dehydration time increased the effectiveness of mass exchange during osmotic dehydration. The addition of chokeberry juice concentrate to standard sucrose solution and the use of ultrasound did not change the value of solid gain and reduced water content. Water activity of the dried apple tissue was not significantly changed after osmotic dehydration, while changes in colour were significant.

Influence of osmotic dehydration pre-treatment and combined drying method on physico-chemical and sensory properties of pomegranate arils, cultivar Mollar de Elche.

PubMed

Cano-Lamadrid, Marina; Lech, Krzysztof; Michalska, Anna; Wasilewska, Malwina; Figiel, Adam; Wojdyło, Aneta; Carbonell-Barrachina, Ángel A

2017-10-01

"Mollar de Elche" is the most popular Spanish pomegranate cultivar (intense sweetness and easy-to-chew arils); however, arils have pale pink colour and flat sensory profile. "Mollar the Elche" arils first underwent an osmotic dehydration pre-treatment (OD) with concentrated juices: (i) chokeberry, (ii) apple, and/or (iii) pomegranate cultivar "Wonderful", to improve their antioxidant capacity, colour, and sensory profile complexity, and later the arils were dried by a combined method (convective pre-drying+vacuum microwave finish drying). The use of OD provided dried arils with characteristic sweetness, and improved colour and aromatic complexity. The recommended OD methods were those using (i) pomegranate, and (ii) pomegranate with chokeberry juices; they improved the total anthocyanin content (mean of 368mgkg -1 ), red colour (a ∗ coordinate 15.6), and antioxidant capacity (e.g. ABTS mean of 5.7mmolTrolox100g -1 ). However, further research is still needed because freeze-dried arils had the highest anthocyanin content. Copyright © 2017 Elsevier Ltd. All rights reserved.

Designing universal primers for the isolation of DNA sequences encoding Proanthocyanidins biosynthetic enzymes in Crataegus aronia

PubMed Central

2012-01-01

Background Hawthorn is the common name of all plant species in the genus Crataegus, which belongs to the Rosaceae family. Crataegus are considered useful medicinal plants because of their high content of proanthocyanidins (PAs) and other related compounds. To improve PAs production in Crataegus tissues, the sequences of genes encoding PAs biosynthetic enzymes are required. Findings Different bioinformatics tools, including BLAST, multiple sequence alignment and alignment PCR analysis were used to design primers suitable for the amplification of DNA fragments from 10 candidate genes encoding enzymes involved in PAs biosynthesis in C. aronia. DNA sequencing results proved the utility of the designed primers. The primers were used successfully to amplify DNA fragments of different PAs biosynthesis genes in different Rosaceae plants. Conclusion To the best of our knowledge, this is the first use of the alignment PCR approach to isolate DNA sequences encoding PAs biosynthetic enzymes in Rosaceae plants. PMID:22883984

Designing universal primers for the isolation of DNA sequences encoding Proanthocyanidins biosynthetic enzymes in Crataegus aronia.

PubMed

Zuiter, Afnan Saeid; Sawwan, Jammal; Al Abdallat, Ayed

2012-08-10

Hawthorn is the common name of all plant species in the genus Crataegus, which belongs to the Rosaceae family. Crataegus are considered useful medicinal plants because of their high content of proanthocyanidins (PAs) and other related compounds. To improve PAs production in Crataegus tissues, the sequences of genes encoding PAs biosynthetic enzymes are required. Different bioinformatics tools, including BLAST, multiple sequence alignment and alignment PCR analysis were used to design primers suitable for the amplification of DNA fragments from 10 candidate genes encoding enzymes involved in PAs biosynthesis in C. aronia. DNA sequencing results proved the utility of the designed primers. The primers were used successfully to amplify DNA fragments of different PAs biosynthesis genes in different Rosaceae plants. To the best of our knowledge, this is the first use of the alignment PCR approach to isolate DNA sequences encoding PAs biosynthetic enzymes in Rosaceae plants.

Electron spin resonance measurement of radical scavenging activity of Aronia melanocarpa fruit juice.

PubMed

Valcheva-Kuzmanova, Stefka; Blagović, Branka; Valić, Srećko

2012-04-01

The fruits of Aronia melanocarpa (Michx.) Elliot contain large amounts of phenolic substances, mainly procyanidins, anthocyanins and other flavonoids, and phenolic acids. The ability of phenolic substances to act as antioxidants has been well established. In this study, we investigated the radical scavenging activity of A. melanocarpa fruit juice (AMFJ). The method used was electron spin resonance (ESR) spectroscopy. The galvinoxyl free radical was used as a scavenging object. AMFJ was added to the galvinoxyl free radical solution. The measure of the radical scavenging activity was the decrease of signal intensity. AMFJ showed a potent antiradical activity causing a strong and rapid decrease of signal intensity as a function of time and juice concentration. This effect of AMFJ was probably due to the activity of its phenolic constituents. The ESR measurements in this study showed a pronounced radical scavenging effect of AMFJ, an important mechanism of its antioxidant activity.

Berry Phenolic Antioxidants – Implications for Human Health?

PubMed Central

Olas, Beata

2018-01-01

Antioxidants present in the diet may have a significant effect on the prophylaxis and progression of various diseases associated with oxidative stress. Berries contain a range of chemical compounds with antioxidant properties, including phenolic compounds. The aim of this review article is to provide an overview of the current knowledge of such phenolic antioxidants, and to discuss whether these compounds may always be natural gifts for human health, based on both in vitro and in vivo studies. It describes the antioxidant properties of fresh berries (including aronia berries, grapes, blueberries, sea buckthorn berries, strawberries and other berries) and their various products, especially juices and wines. Some papers report that these phenolic compounds may sometimes behave like prooxidants, and sometimes demonstrate both antioxidant and prooxidant activity, while others note they do not behave the same way in vitro and in vivo. However, no unwanted or toxic effects (i.e., chemical, hematological or urinary effect) have been associated with the consumption of berries or berry juices or other extracts, especially aronia berries and aronia products in vivo, and in vitro, which may suggest that the phenolic antioxidants found in berries are natural gifts for human health. However, the phenolic compound content of berries and berry products is not always well described, and further studies are required to determine the therapeutic doses of different berry products for use in future clinical studies. Moreover, further experiments are needed to understand the beneficial effects reported so far from the mechanistic point of view. Therefore, greater attention should be paid to the development of well-controlled and high-quality clinical studies in this area. PMID:29662448

[Effect of anthocyanins from Aronia melanocarpa on blood pressure, concentration of endothelin-1 and lipids in patients with metabolic syndrome].

PubMed

Broncel, Marlena; Koziróg-Kołacińska, Marzena; Andryskowski, Grzegorz; Duchnowicz, Piotr; Koter-Michalak, Maria; Owczarczyk, Aleksandra; Chojnowska-Jezierska, Julita

2007-08-01

To estimate the influence of anthocyanins from Aronia melanocarpa on blood pressure, concentration of endothelin-1 (ET-1), serum lipids, fasting glucose, uric acid and membrane cholesterol in erythrocytes of patients (pts) with metabolic syndrome (MS). The study comprised 22 healthy volunteers and 25 pts with MS treated with anthocyanins (3 x 100 mg/d) for 2 months. Waist circumference (> or = 80 cm for women and > or =94 cm for men), triglicerydes (TG) level >150 mg/dl (1.7 mmol/l), cholesterol-HDL (HDL-C) level < 40 mg/dl (1.0 mmol/l) for men and <50 mg/dl (1.3 mmol/l) for women, systolic blood pressure (SBP) >130 mmHg and/or diastolic blood pressure (DBP) >85 mmHg were inclusion criteria for patients with MS. Before and after 2 months of treatment the following parameters were determined: SBP, DBP, serum lipids (total cholesterol--TC, cholesterol LDL--LDL-C, cholesterol HDL--HDL-C, TG--by enzymatic method), membrane cholesterol in erythrocytes (method of IIcy), ET-1 (immunoenzymatic method), fasting glucose level was (colorimetric method), uric acid (enzymatic--colorimetric method). After two months therapy of anthocyanins from Aronia melanocarpa in comparison with baseline it was observed a significant decrease of: SBP (144.20 +/- 9.97 vs. 131.83 +/- 12.24 mmHg, p < 0.001) and DBP (87.20 +/- 9.9 vs. 82.13 +/- 10.33 mmHg, p < 0.05), TC (242.80 +/- 34.48 vs. 227.96 +/- 33.07 mg/dl, p < 0.001), LDL-C (158.71 +/- 35.78 vs. 146.21 +/- 34.63 mg/dl, p < 0.01), TG (215.92 +/- 63.61 vs. 187.58 +/- 90 mg/dl, p < 0.05), ET-1 (2.44 +/- 0.51 vs. 1.74 +/- 0.42 pg/ml, p < 0.001) and membrane cholesterol (4.85 +/- 0.65 vs. 2.81 +/- 0.54 mmol/Lpc, p < 0.001), uric acid and fasting blood glucose levels did not change significantly after study cessation. The results of our study show that anthocyanins from Aronia melanocarpa may be of benefit to patients with MS as for as atherosclerosis prevention is concerned. It seems to result from anthocyanins influence on blood pressure, serum lipid and endothelin-1 level.

Optimization of ultrasound-assisted aqueous two-phase system extraction of polyphenolic compounds from Aronia melanocarpa pomace by response surface methodology.

PubMed

Xu, Yan-Yang; Qiu, Yang; Ren, Hui; Ju, Dong-Hu; Jia, Hong-Lei

2017-03-16

Aronia melanocarpa berries are abundant in polyphenolic compounds. After juice production, the pomace of pressed berries still contains a substantial amount of polyphenolic compounds. For efficient utilization of A. melanocarpa berries and the enhancement of polyphenolic compound yields in Aronia melanocarpa pomace (AMP), total phenolics (TP) and total flavonoids (TF) from AMP were extracted, using ultrasound-assisted aqueous two-phase system (UAE-ATPS) extraction method. First, the influences of ammonium sulfate concentration, ethanol-water ratio, ultrasonic time, and ultrasonic power on TP and TF yields were investigated. On this basis, process variables such as ammonium sulfate concentration (0.30-0.35 g mL -1 ), ethanol-water ratio (0.6-0.8), ultrasonic time (40-60 min), and ultrasonic power (175-225 W) were further optimized by implementing Box-Benhnken design with response surface methodology. The experimental results showed that optimal extraction conditions of TP from AMP were as follows: ammonium sulfate concentration of 0.324 g mL -1 , ethanol-water ratio of 0.69, ultrasonic time of 52 min, and ultrasonic power of 200 W. Meanwhile, ammonium sulfate concentration of 0.320 g mL -1 , ethanol-water ratio of 0.71, ultrasonic time of 50 min, and ultrasonic power of 200 W were determined as optimum extraction conditions of TF in AMP. Experimental validation was performed, where TP and TF yields reached 68.15 ± 1.04 and 11.67 ± 0.63 mg g -1 , respectively. Close agreement was found between experimental and predicted values. Overall, the present results demonstrated that ultrasound-assisted aqueous two-phase system extraction method was successfully used to extract total phenolics and flavonoids in A. melanocarpa pomace.

Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel.

PubMed

Ljubuncic, Predrag; Portnaya, Irina; Cogan, Uri; Azaizeh, Hassan; Bomzon, Arieh

2005-10-03

The medicinal use of extracts prepared from plant parts of the genus Crataegus dates back to ancient times. Furthermore, it has been proposed that its antioxidant constituents account for its beneficial therapeutic effects. A decoction of leaves and unripe fruits from Crataegus aronia syn. azarolus (L) (Rosaceae), the indigenous Israeli hawthorn, is used to treat cardiovascular diseases, cancer, diabetes and sexual weakness in Arab traditional medicine. Because laboratory data on the bioactivity of extracts prepared from the indigenous Israeli hawthorn is lacking, we evaluated the antioxidant and cytotoxic potentials of an extract prepared from leaves and unripe fruits in a variety of cell and cell-free in vitro assays. The antioxidant assays measured: (a) its ability to inhibit (i) oxidation of beta-carotene, (ii) 2,2'-azobis(2-amidino-propan) dihydrochloride (AAPH)-induced plasma oxidation and (iii) iron-induced lipid peroxidation in rat liver homogenates; (b) its ability to scavenge the superoxide (O2-) radical; (c) its effects on the enzyme xanthine oxidase (XO) activity; (d) its effect on the redox state of glutathione (GSH) in cultured Hep G2 cells. In addition, we also evaluated the effects of the extract on cell membrane integrity and mitochondrial respiration in cultured Hep G2 cells. Water-soluble extracts inhibited (1) oxidation of beta-carotene, (2) AAPH-induced plasma oxidation and (3) Fe(2+)-induced lipid peroxidation in rat liver homogenates. In addition, the extract (4) is an efficient scavenger of the O2- (5) increases intracellular GSH levels and (6) is not cytotoxic. Accordingly, we propose that the therapeutic benefit of Crataegus aronia can be, at least in part, attributed to its effective inhibition of oxidative processes, efficient scavenging of O2- and possible increasing GSH biosynthesis.

Electron spin resonance measurement of radical scavenging activity of Aronia melanocarpa fruit juice

PubMed Central

Valcheva-Kuzmanova, Stefka; Blagović, Branka; Valić, Srećko

2012-01-01

Background: The fruits of Aronia melanocarpa (Michx.) Elliot contain large amounts of phenolic substances, mainly procyanidins, anthocyanins and other flavonoids, and phenolic acids. The ability of phenolic substances to act as antioxidants has been well established. Objective: In this study, we investigated the radical scavenging activity of A. melanocarpa fruit juice (AMFJ). Materials and Methods: The method used was electron spin resonance (ESR) spectroscopy. The galvinoxyl free radical was used as a scavenging object. AMFJ was added to the galvinoxyl free radical solution. The measure of the radical scavenging activity was the decrease of signal intensity. Results: AMFJ showed a potent antiradical activity causing a strong and rapid decrease of signal intensity as a function of time and juice concentration. This effect of AMFJ was probably due to the activity of its phenolic constituents. Conclusion: The ESR measurements in this study showed a pronounced radical scavenging effect of AMFJ, an important mechanism of its antioxidant activity. PMID:22701293

Immunomodulating Activity of Aronia melanocarpa Polyphenols

PubMed Central

Ho, Giang T. T.; Bräunlich, Marie; Austarheim, Ingvild; Wangensteen, Helle; Malterud, Karl E.; Slimestad, Rune; Barsett, Hilde

2014-01-01

The immunomodulating effects of isolated proanthocyanidin-rich fractions, procyanidins C1, B5 and B2 and anthocyanins of Aronia melanocarpa were investigated. In this work, the complement-modulating activities, the inhibitory activities on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophages and effects on cell viability of these polyphenols were studied. Several of the proanthocyanidin-rich fractions, the procyanidins C1, B5 and B2 and the cyanidin aglycone possessed strong complement-fixing activities. Cyanidin 3-glucoside possessed stronger activity than the other anthocyanins. Procyanidins C1, B5 and B2 and proanthocyanidin-rich fractions having an average degree of polymerization (PD) of 7 and 34 showed inhibitory activities on NO production in LPS-stimulated RAW 264.7 mouse macrophages. All, except for the fraction containing proanthocyanidins with PD 34, showed inhibitory effects without affecting cell viability. This study suggests that polyphenolic compounds of A. melanocarpa may have beneficial effects as immunomodulators and anti-inflammatory agents. PMID:24983479

Neuroprotective effect of Aronia melanocarpa extract against glutamate-induced oxidative stress in HT22 cells.

PubMed

Lee, Hyeon Yong; Weon, Jin Bae; Ryu, Gahee; Yang, Woo Seung; Kim, Nam Young; Kim, Myong Ki; Ma, Choong Je

2017-04-11

Glutamate (an endogenous excitatory neurotransmitter) at high concentrations contributes to the development of neurodegenerative diseases. Aronia melanocarpa (A. melanocarpa) berries contain anthocyanins and have high antioxidant activities. In this study, we evaluated whether A. melanocarpa berries could protect neuronal cells against glutamate-induced oxidative stress. A. melanocarpa berries exerted a protective effect against cytotoxicity in HT22 mouse hippocampal cells by MTT assay. We evaluated oxidative stress parameters including ROS level, intracellular Ca 2+ level, glutathione level and antioxidant enzyme activity in HT22 cells to elucidate the mechanism of its neuroprotective effect. A. melanocarpa berries decreased glutamate-induced death of HT22 cells. In addition, A. melanocarpa berries reduced ROS and intracellular Ca 2+ levels. Glutathione level, antioxidant enzymes, glutathione reductase and glutathione peroxide activities and mitochondrial membrane potential were also increased in HT22 cells. These results suggested that A. melanocarpa berries protected HT22 cells by exerting an antioxidant effect.

Immunomodulating activity of Aronia melanocarpa polyphenols.

PubMed

Ho, Giang T T; Bräunlich, Marie; Austarheim, Ingvild; Wangensteen, Helle; Malterud, Karl E; Slimestad, Rune; Barsett, Hilde

2014-06-30

The immunomodulating effects of isolated proanthocyanidin-rich fractions, procyanidins C1, B5 and B2 and anthocyanins of Aronia melanocarpa were investigated. In this work, the complement-modulating activities, the inhibitory activities on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophages and effects on cell viability of these polyphenols were studied. Several of the proanthocyanidin-rich fractions, the procyanidins C1, B5 and B2 and the cyanidin aglycone possessed strong complement-fixing activities. Cyanidin 3-glucoside possessed stronger activity than the other anthocyanins. Procyanidins C1, B5 and B2 and proanthocyanidin-rich fractions having an average degree of polymerization (PD) of 7 and 34 showed inhibitory activities on NO production in LPS-stimulated RAW 264.7 mouse macrophages. All, except for the fraction containing proanthocyanidins with PD 34, showed inhibitory effects without affecting cell viability. This study suggests that polyphenolic compounds of A. melanocarpa may have beneficial effects as immunomodulators and anti-inflammatory agents.

Discovery of human urinary biomarkers of aronia-citrus juice intake by HPLC-q-TOF-based metabolomic approach.

PubMed

Llorach, Rafael; Medina, Sonia; García-Viguera, Cristina; Zafrilla, Pilar; Abellán, José; Jauregui, Olga; Tomás-Barberán, Francisco A; Gil-Izquierdo, Angel; Andrés-Lacueva, Cristina

2014-06-01

Metabolomics has emerged in the field of food and nutrition sciences as a powerful tool for doing profiling approaches. In this context, HPLC-q-TOF-based metabolomics approach was applied to unveil changes in the urinary metabolome in human subjects (n = 51, 23 men and 28 women) after regular aronia-citrus juice (AC-juice) intake (250 mL/day) during 16 weeks compared to individuals given a placebo beverage. Samples were analyzed by HPLC-q-TOF followed by multivariate data analysis (orthogonal signal filtering-partial least square discriminant analysis) that discriminated relevant mass features related to AC-juice intake. The results showed that biomarkers of AC-juice intake including metabolites coming from metabolism of food components as proline betaine, ferulic acid, and two unknown mercapturate derivatives were identified. Discovery of new biomarkers of food intake will help in the building up of the food metabolome and facilitate future insights into the mechanisms of action of dietary components in population health. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Extracts, Anthocyanins and Procyanidins from Aronia melanocarpa as Radical Scavengers and Enzyme Inhibitors

PubMed Central

Bräunlich, Marie; Slimestad, Rune; Wangensteen, Helle; Brede, Cato; Malterud, Karl E.; Barsett, Hilde

2013-01-01

Extracts, subfractions, isolated anthocyanins and isolated procyanidins B2, B5 and C1 from the berries and bark of Aronia melanocarpa were investigated for their antioxidant and enzyme inhibitory activities. Four different bioassays were used, namely scavenging of the diphenylpicrylhydrazyl (DPPH) radical, inhibition of 15-lipoxygenase (15-LO), inhibition of xanthine oxidase (XO) and inhibition of α-glucosidase. Among the anthocyanins, cyanidin 3-arabinoside possessed the strongest and cyanidin 3-xyloside the weakest radical scavenging and enzyme inhibitory activity. These effects seem to be influenced by the sugar units linked to the anthocyanidin. Subfractions enriched in procyanidins were found to be potent α-glucosidase inhibitors; they possessed high radical scavenging properties, strong inhibitory activity towards 15-LO and moderate inhibitory activity towards XO. Trimeric procyanidin C1 showed higher activity in the biological assays compared to the dimeric procyanidins B2 and B5. This study suggests that different polyphenolic compounds of A. melanocarpa can have beneficial effects in reducing blood glucose levels due to inhibition of α-glucosidase and may have a potential to alleviate oxidative stress. PMID:23459328

Extracts, anthocyanins and procyanidins from Aronia melanocarpa as radical scavengers and enzyme inhibitors.

PubMed

Bräunlich, Marie; Slimestad, Rune; Wangensteen, Helle; Brede, Cato; Malterud, Karl E; Barsett, Hilde

2013-03-04

Extracts, subfractions, isolated anthocyanins and isolated procyanidins B2, B5 and C1 from the berries and bark of Aronia melanocarpa were investigated for their antioxidant and enzyme inhibitory activities. Four different bioassays were used, namely scavenging of the diphenylpicrylhydrazyl (DPPH) radical, inhibition of 15-lipoxygenase (15-LO), inhibition of xanthine oxidase (XO) and inhibition of α-glucosidase. Among the anthocyanins, cyanidin 3-arabinoside possessed the strongest and cyanidin 3-xyloside the weakest radical scavenging and enzyme inhibitory activity. These effects seem to be influenced by the sugar units linked to the anthocyanidin. Subfractions enriched in procyanidins were found to be potent α-glucosidase inhibitors; they possessed high radical scavenging properties, strong inhibitory activity towards 15-LO and moderate inhibitory activity towards XO. Trimeric procyanidin C1 showed higher activity in the biological assays compared to the dimeric procyanidins B2 and B5. This study suggests that different polyphenolic compounds of A. melanocarpa can have beneficial effects in reducing blood glucose levels due to inhibition of α-glucosidase and may have a potential to alleviate oxidative stress.

Antihypertensive Effects of Hydroalcoholic Extract of Crataegus Azarolus Subspecies Aronia Fruit in Rats with Renovascular Hypertension: An Experimental Mechanistic Study

PubMed Central

Haydari, Mohammad Reza; Panjeshahin, Mohammad Reza; Mashghoolozekr, Elaheh; Nekooeian, Ali Akbar

2017-01-01

Background: Hawthorn species decreases blood pressure and relaxes precontracted vessels. This study aimed at examining the antihypertensive effect and related mechanisms of hydroalcoholic extract of Crataegus azarolus subspecies aronia fruit in rats with renovascular hypertension. Methods: Six groups of male Sprague-Dawley rats, each containing 6 to 8 rats, were studied. The groups comprised of one sham group and 5 renal artery-clipped groups. The sham group received vehicle (distilled water 0.5 ml/day) and the renal artery-clipped groups received vehicle or the extract at 5, 10, 20 or 30 mg/kg/day. Oral vehicle or extract was administered daily for 4 weeks following sham-operation or induction of hypertension. Systolic blood pressure and heart rate were measured weekly. Isolated aorta study was performed by last week and serum superoxide dismutase and glutathione reductase were measured. The findings were analyzed using one-way analysis of variance and Duncan’s multiple range tests at P≤0.05 using SigmaStat software. Results: The data obtained after 4 weeks of treatment showed that the renal artery-clipped group receiving vehicle had significantly higher systolic blood pressure (P=0.002) and phenylephrine maximal response (P=0.01); and lower acetylcholine maximal response (P=0.01), serum superoxide dismutase (P=0.006) and serum glutathione reductase (P=0.006) than those of the sham group. The renal artery-clipped group receiving extract had significantly lower systolic blood pressure (P=0.03) and phenylephrine maximal response (P=0.01); and significantly higher acetylcholine maximal response (P=0.01), serum superoxide dismutase (P=0.015), and serum glutathione reductase (P=0.015) than those of the renal artery-clipped group receiving vehicle. Conclusion: Our findings show that the hydroalcoholic extract of Crataegus azarolus subspecies aronia fruit has antihypertensive effects, which may be partly due to antioxidant and nitric oxide releasing effects. PMID:28533575

Antihypertensive Effects of Hydroalcoholic Extract of Crataegus Azarolus Subspecies Aronia Fruit in Rats with Renovascular Hypertension: An Experimental Mechanistic Study.

PubMed

Haydari, Mohammad Reza; Panjeshahin, Mohammad Reza; Mashghoolozekr, Elaheh; Nekooeian, Ali Akbar

2017-05-01

Hawthorn species decreases blood pressure and relaxes precontracted vessels. This study aimed at examining the antihypertensive effect and related mechanisms of hydroalcoholic extract of Crataegus azarolus subspecies aronia fruit in rats with renovascular hypertension. Six groups of male Sprague-Dawley rats, each containing 6 to 8 rats, were studied. The groups comprised of one sham group and 5 renal artery-clipped groups. The sham group received vehicle (distilled water 0.5 ml/day) and the renal artery-clipped groups received vehicle or the extract at 5, 10, 20 or 30 mg/kg/day. Oral vehicle or extract was administered daily for 4 weeks following sham-operation or induction of hypertension. Systolic blood pressure and heart rate were measured weekly. Isolated aorta study was performed by last week and serum superoxide dismutase and glutathione reductase were measured. The findings were analyzed using one-way analysis of variance and Duncan's multiple range tests at P≤0.05 using SigmaStat software. The data obtained after 4 weeks of treatment showed that the renal artery-clipped group receiving vehicle had significantly higher systolic blood pressure (P=0.002) and phenylephrine maximal response (P=0.01); and lower acetylcholine maximal response (P=0.01), serum superoxide dismutase (P=0.006) and serum glutathione reductase (P=0.006) than those of the sham group. The renal artery-clipped group receiving extract had significantly lower systolic blood pressure (P=0.03) and phenylephrine maximal response (P=0.01); and significantly higher acetylcholine maximal response (P=0.01), serum superoxide dismutase (P=0.015), and serum glutathione reductase (P=0.015) than those of the renal artery-clipped group receiving vehicle. Our findings show that the hydroalcoholic extract of Crataegus azarolus subspecies aronia fruit has antihypertensive effects, which may be partly due to antioxidant and nitric oxide releasing effects.

Sensory qualities of pastry products enriched with dietary fiber and polyphenolic substances.

PubMed

Komolka, Patrycja; Górecka, Danuta; Szymandera-Buszka, Krystyna; Jędrusek-Golińska, Anna; Dziedzic, Krzysztof; Waszkowiak, Katarzyna

2016-01-01

Growing consumer demand for products with pro-health properties is forcing food manufacturers to introduce new food items onto the market, which will not only possess such health-enhancing properties but will also compete on the grounds of sensory attributes - taste, flavour, texture etc. The aim was to evaluate these sensory attributes of pastry products enhanced with biologically active compounds, such as inulin, buckwheat hull and buckwheat flour. For decreasing the energy value of the products tested (crispy cookies, muesli cookies, waffles and pancakes) some ingredients were replaced: vegetable butter or oil by inulin and wheat flour by roasted buckwheat flour and thermally processed buckwheat hull. The substances mentioned are rich sources of soluble and insoluble buckwheat fiber, and also polyphenolic substances. Dry chokeberry and mulberry leaf extract were added as a rich source of flavonoids and 1-deoxynorijimycin, respectively. These substances are recommended for people with obesity. The processing was carried out at 175°C for 15 minutes using a convection oven (Rational Combi-Steamer CCC). Pastry products with buckwheat flour, buckwheat hulls, mulberry extract, chokeberry and inulin had a lower food energy, a higher dietary fiber content and scored high on customer desirability. Pastry products which contain ingredients carrying biologically active substances are not only attractive from the sensory point of view, but also low in calories, and are thus recommendable for obesity people.

Anthocyanins in chokeberry and purple maize attenuate diet-induced metabolic syndrome in rats.

PubMed

Bhaswant, Maharshi; Shafie, Siti Raihanah; Mathai, Michael L; Mouatt, Peter; Brown, Lindsay

2017-09-01

Increased consumption of fruits and vegetables as functional foods leads to the reduction of signs of metabolic syndrome. The aim of this study was to measure and compare cardiovascular, liver, and metabolic parameters following chronic administration of the same dose of anthocyanins either from chokeberry (CB) or purple maize (PM) in rats with diet-induced metabolic syndrome. Male Wistar rats were fed a maize starch (C) or high-carbohydrate, high-fat diet (H) and divided into six groups for 16 wk. The rats were fed C, C with CB or PM for the last 8 wk (CCB or CPM), H, H with CB or PM for the last 8 wk (HCB or HPM); CB and PM rats received ∼8 mg anthocyanins/kg daily. The rats were monitored for changes in blood pressure, cardiovascular and hepatic structure and function, glucose tolerance, and adipose tissue mass. HCB and HPM rats showed reduced visceral adiposity index, total body fat mass, and systolic blood pressure; improved glucose tolerance, liver, and cardiovascular structure and function; decreased plasma triacylglycerols and total cholesterol compared with H rats. Inflammatory cell infiltration was reduced in heart and liver. CB and PM interventions gave similar responses, suggesting that anthocyanins are the bioactive molecules in the attenuation or reversal of metabolic syndrome by prevention of inflammation-induced damage. Copyright © 2016 Elsevier Inc. All rights reserved.

Evaluation of anthocyanins in Aronia melanocarpa/BSA binding by spectroscopic studies.

PubMed

Wei, Jie; Xu, Dexin; Zhang, Xiao; Yang, Jing; Wang, Qiuyu

2018-05-02

The interaction between Anthocyanins in Aronia melanocarpa (AMA) and bovine serum albumin (BSA) were studied in this paper by multispectral technology, such as fluorescence quenching titration, circular dichroism (CD) spectroscopy and Fourier transform infrared spectroscopy (FTIR). The results of the fluorescence titration revealed that AMA could strongly quench the intrinsic fluorescence of BSA by static quenching. The apparent binding constants K SV and number of binding sites n of AMA with BSA were obtained by fluorescence quenching method. The thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS), were calculated to be 18.45 kJ mol -1  > 0 and 149.72 J mol -1  K -1  > 0, respectively, which indicated that the interaction of AMA with BSA was driven mainly by hydrophobic forces. The binding process was a spontaneous process of Gibbs free energy change. Based on Förster's non-radiative energy transfer theory, the distance r between the donor (BSA) and the receptor (AMA) was calculated to be 3.88 nm. Their conformations were analyzed using infrared spectroscopy and CD. The results of multispectral technology showed that the binding of AMA to BSA induced the conformational change of BSA.

Aronia melanocarpa as a protector against nitration of fibrinogen.

PubMed

Bijak, Michał; Saluk, Joanna; Antosik, Adam; Ponczek, Michał B; Żbikowska, Halina M; Borowiecka, Marta; Nowak, Paweł

2013-04-01

Fibrinogen (Fg) also known as coagulation factor I represents about 4% of the total human plasma proteins. The main function of Fg is its involvement in last phase of blood coagulation cascade, when thrombin-induced conversion of dissolved plasma fibrinogen into an insoluble fibrin clot occurs. The reaction of fibrinogen with peroxynitrite causes both structural modifications and changes of the biological properties of this plasma glycoprotein. Recently, there is an increased interest in the screening of natural products present in fruits, vegetables and herbs for their possible antioxidative activities. Therefore, the aim of our study was to estimate the effect of extract from berries of Aronia melanocarpa against nitrative and oxidative damage induced by peroxynitrite. The extract from A. melanocarpa (0.5-50 μg/ml) added to Fg 10 min before peroxynitrite (100 μM) significantly inhibited both the formation of the high molecular weight protein aggregates and nitration of Fg molecule. The extract also abolished peroxynitrite-induced inhibition of fibrinogen polymerization (by 95% at 50 μg/ml). The obtained results indicate that natural extract from berries of A. melanocarpa has protective effects against peroxynitrite-induced nitrative damage of plasma fibrinogen, and therefore may contribute in the prevention of peroxynitrite-related cardiovascular or inflammatory diseases. Copyright © 2013 Elsevier B.V. All rights reserved.

Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in streptozotocin-induced diabetic rats.

PubMed

Valcheva-Kuzmanova, S; Kuzmanov, K; Tancheva, S; Belcheva, A

2007-03-01

Aronia melanocarpa fruit juice (AMFJ) is rich in phenolic antioxidants, especially flavonoids from the anthocyanin subclass. The aim of the present study was to investigate the influence of AMFJ on plasma glucose and lipids in diabetic rats. Diabetes was induced by an intraperitoneal injection of streptozotocin (50 mg/kg). AMFJ was applied by gavage at doses of 10 and 20 ml/kg for 6 weeks to normal and diabetic rats. Streptozotocin caused a significant elevation of plasma glucose by 141% and of plasma triglycerides (TG) by 64% in comparison with normal control rats and induced statistically insignificant elevations of total cholesterol and LDL-cholesterol and a reduction of HDL-cholesterol. Applied to normal rats, AMFJ did not influence plasma glucose and lipid levels. Applied to diabetic rats, AMFJ (10 and 20 ml/kg) significantly reduced plasma glucose by 44% and 42% and TG by 35% and 39%, respectively, to levels that did not significantly differ from those of the normal control rats and counteracted the influence of streptozotocin on total cholesterol, LDL-cholesterol and HDL-cholesterol. In conclusion, AMFJ significantly decreased the streptozotocin-induced abnormalities in blood glucose and TG in diabetic rats and might be useful in prevention and control of diabetes mellitus and diabetes-associated complications. Copyright 2007 Prous Science.

Anthocyanin-rich Aronox extract from Aronia melanocarpa E protects against 7beta-hydroxycholesterol-induced apoptosis of endothelial cells.

PubMed

Zapolska-Downar, Danuta; Nowicka, Grazyna; Sygitowicz, Grazyna; Jarosz, Mirosław

2008-01-01

It has been previously reported that dietary polyphenolic compounds have a positive effect in cardiovascular diseases, although the underlying mechanisms remain unknown. In this study, the effects of Aronox, an anthocyanin-rich extract from Aronia melanocarpa E, on human umbilical vein endothelial cell (HUVEC) apoptosis was investigated. Apoptosis was measured in HUVEC pretreated with Aronox and than treated with 7beta-hydroxycholesterol (20 microg/ml). For the evaluation of apoptosis we used the cell binding of annexin V, the propidium iodide uptake method and the TUNEL assay. We also measured the generation of reactive oxygen species (ROS) in the cells, using fluorescent probe 2',7'-dichlorofluorescein acetate. Moreover, alteration of the mitochondrial membrane potential with subsequent cytochrome c release, activation of caspase-3, and the level of anti-apoptotic Bcl-2 were investigated. Aronox significantly decreased 7beta-hydroxycholesterol-induced apoptosis and ROS generation, and ameliorated the collapse of the mitochondrial transmembrane potential with subsequent inhibition of cytochrome c release. Furthermore, down-regulation of Bcl-2 and up-regulation of caspase-3 activation were reversed by Aronox. The protective action of the extract against apoptosis in endothelial cells may contribute to the potential benefits drawn from consumption of plant polyphenols. Copyright 2009 S. Karger AG, Basel.

An extract from berries of Aronia melanocarpa modulates the generation of superoxide anion radicals in blood platelets from breast cancer patients.

PubMed

Kedzierska, Magdalena; Olas, Beata; Wachowicz, Barbara; Stochmal, Anna; Oleszek, Wieslaw; Jeziorski, Arkadiusz; Piekarski, Janusz; Glowacki, Rafal

2009-10-01

Plant antioxidants protect cells against oxidative stress. Because oxidative stress (measured by different biomarkers) is observed in breast cancer patients, the aim of this study was to establish the effects of a polyphenol-rich extract of Aronia melanocarpa (final concentration of 50 microg/mL, 5 min, 37 degrees C) on superoxide anion radicals (O(2)(-*)) and glutathione (GSH) in platelets from patients with breast cancer and in a healthy group in vitro. Generation of O(2)(-*) in platelets before and after incubation with the extract was measured by cytochrome C reduction. Using HPLC, we determined the level of glutathione in blood platelets. We observed a statistically significant increase of biomarkers of oxidative stress such as O(2)(-*) and a decrease in GSH in platelets from patients with breast cancer compared with the healthy group. We showed that the extract from A. melanocarpa added to blood platelets significantly reduced the production of O(2)(-*) in platelets not only from the healthy group but also from patients with breast cancer. Considering the data presented in this study, we have demonstrated the protective role of the extract from A. melanocarpa in patients with breast cancer in vitro. Georg Thieme Verlag KG Stuttgart-New York.

Cognitive-Enhancing Effect of Aronia melanocarpa Extract against Memory Impairment Induced by Scopolamine in Mice

PubMed Central

Lee, Hyeon Yong; Weon, Jin Bae; Jung, Youn Sik; Kim, Nam Young; Kim, Myong Ki; Ma, Choong Je

2016-01-01

Aronia melanocarpa (A. melanocarpa) berries are a fruit with a marked antioxidant effect. The objective of this study was to confirm the effect of A. melanocarpa berries extract against scopolamine-induced memory impairment in mice using the Morris water maze and passive avoidance test. Moreover, we determined a possible mechanism of the cognitive-enhancing effect involving AChE activity and BDNF and p-CREB expression in the hippocampus of mice. A. melanocarpa berries extract attenuated the learning and memory impairment induced by scopolamine in the Morris water maze (79.3 ± 0.8 s of 200 mg/kg and 64.4 ± 10.7 s of 400 mg/kg on day 4) and passive avoidance tests (46.0 ± 41.1 s of 200 mg/kg and 25.6 ± 18.7 s of 400 mg/kg). A. melanocarpa berries extract reduced the acetylcholinesterase level in the hippocampus of scopolamine-injected mice and increased BDNF and p-CREB expression in the hippocampus. The major compound, cyanidin-3-O-galactoside, also reversed memory impairment. These results showed that A. melanocarpa berries extract improved memory impairment by inhibiting AChE and increasing BDNF and p-CREB expression, and cyanidin-3-O-galactoside may be responsible for the effect of A. melanocarpa berries extract. PMID:27239211

Effects of Glucomannan-Enriched, Aronia Juice-Based Supplement on Cellular Antioxidant Enzymes and Membrane Lipid Status in Subjects with Abdominal Obesity

PubMed Central

Petrović-Oggiano, Gordana; Glibetić, Natalija; Zec, Manja; Debeljak-Martacic, Jasmina; Konić-Ristić, Aleksandra

2014-01-01

The aim of this study was to analyze the effects of a 4-week-long consumption of glucomannan-enriched, aronia juice-based supplement on anthropometric parameters, membrane fatty acid profile, and status of antioxidant enzymes in erythrocytes obtained from postmenopausal women with abdominal obesity. Twenty women aged 45–65 with a mean body mass index (BMI) of 36.1 ± 4.4 kg/m2 and waist circumference of 104.8 ± 10.1 cm were enrolled. Participants were instructed to consume 100 mL of supplement per day as part of their regular diet. A significant increase in the content of n-3 (P < 0.05) polyunsaturated fatty acids in membrane phospholipids was observed, with a marked increase in the level of docosahexaenoic fatty acid (P < 0.05). Accordingly, a decrease in the n-6 and n-3 fatty acids ratio was observed (P < 0.05). The observed effects were accompanied with an increase in glutathione peroxidase activity (P < 0.05). Values for BMI (P < 0.001), waist circumference (P < 0.001), and systolic blood pressure (P < 0.05) were significantly lower after the intervention. The obtained results indicate a positive impact of tested supplement on cellular oxidative damage, blood pressure, and anthropometric indices of obesity. PMID:25574495

Cognitive-Enhancing Effect of Aronia melanocarpa Extract against Memory Impairment Induced by Scopolamine in Mice.

PubMed

Lee, Hyeon Yong; Weon, Jin Bae; Jung, Youn Sik; Kim, Nam Young; Kim, Myong Ki; Ma, Choong Je

2016-01-01

Aronia melanocarpa (A. melanocarpa) berries are a fruit with a marked antioxidant effect. The objective of this study was to confirm the effect of A. melanocarpa berries extract against scopolamine-induced memory impairment in mice using the Morris water maze and passive avoidance test. Moreover, we determined a possible mechanism of the cognitive-enhancing effect involving AChE activity and BDNF and p-CREB expression in the hippocampus of mice. A. melanocarpa berries extract attenuated the learning and memory impairment induced by scopolamine in the Morris water maze (79.3 ± 0.8 s of 200 mg/kg and 64.4 ± 10.7 s of 400 mg/kg on day 4) and passive avoidance tests (46.0 ± 41.1 s of 200 mg/kg and 25.6 ± 18.7 s of 400 mg/kg). A. melanocarpa berries extract reduced the acetylcholinesterase level in the hippocampus of scopolamine-injected mice and increased BDNF and p-CREB expression in the hippocampus. The major compound, cyanidin-3-O-galactoside, also reversed memory impairment. These results showed that A. melanocarpa berries extract improved memory impairment by inhibiting AChE and increasing BDNF and p-CREB expression, and cyanidin-3-O-galactoside may be responsible for the effect of A. melanocarpa berries extract.

The Effects of Aronia melanocarpa 'Viking' Extracts in Attenuating RANKL-Induced Osteoclastic Differentiation by Inhibiting ROS Generation and c-FOS/NFATc1 Signaling.

PubMed

Ghosh, Mithun; Kim, In Sook; Lee, Young Min; Hong, Seong Min; Lee, Taek Hwan; Lim, Ji Hong; Debnath, Trishna; Lim, Beong Ou

2018-03-08

This study aimed to determine the anti-osteoclastogenic effects of extracts from Aronia melanocarpa 'Viking' (AM) and identify the underlying mechanisms in vitro. Reactive oxygen species (ROS) are signal mediators in osteoclast differentiation. AM extracts inhibited ROS production in RAW 264.7 cells in a dose-dependent manner and exhibited strong radical scavenging activity. The extracts also attenuated the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts. To attain molecular insights, the effect of the extracts on the signaling pathways induced by receptor activator of nuclear factor kappa B ligand (RANKL) were also investigated. RANKL triggers many transcription factors through the activation of mitogen-activated protein kinase (MAPK) and ROS, leading to the induction of osteoclast-specific genes. The extracts significantly suppressed RANKL-induced activation of MAPKs, such as extracellular signal-regulated kinase (ERK), c-Jun- N -terminal kinase (JNK) and p38 and consequently led to the downregulation of c-Fos and nuclear factor of activated T cells 1 (NFATc1) protein expression which ultimately suppress the activation of the osteoclast-specific genes, cathepsin K, TRAP, calcitonin receptor and integrin β₃. In conclusion, our findings suggest that AM extracts inhibited RANKL-induced osteoclast differentiation by downregulating ROS generation and inactivating JNK/ERK/p38, nuclear factor kappa B (NF-κB)-mediated c-Fos and NFATc1 signaling pathway.

Aronia melanocarpa fruit juice ameliorates the symptoms of inflammatory bowel disease in TNBS-induced colitis in rats.

PubMed

Valcheva-Kuzmanova, Stefka; Kuzmanov, Atanas; Kuzmanova, Vasilena; Tzaneva, Maria

2018-03-01

Trinitrobenzensulfonic acid (TNBS) is commonly used to induce an experimental inflammatory bowel disease (IBD) model. Oxidative stress and inflammation have been proposed as mechanisms underlying the pathophysiology of IBD. Aronia melanocarpa fruit juice (AMFJ) is extremely rich in polyphenolic substances, mainly proanthocyanidins, flavonoids and phenolic acids. The aim of this study was to evaluate the effect of AMFJ in a rat TNBSinduced colitis model and to compare the effect of the juice with that of sulfasalazine. Colitis was induced by TNBS in male Wistar rats. After the induction of colitis, AMFJ at three doses (2.5, 5 and 10 mL/kg) and sulfasalazine (400 mg/kg) were administered orally till the 14th experimental day. Severity of colitis was assessed by macroscopic and histopathological criteria. Oxidative stress was evaluated by the concentration of thiobarbituric acid reactive substances (TBARS). TNBS caused severe colonic damage. AMFJ dose-dependently ameliorated TNBS-induced colitis. It improved the macroscopic and microscopic signs of colitis, and prevented the increase of colonic TBARS concentrations. Regarding different indices, the effect of AMFJ was comparable or even higher than that of sulfasalazine. In conclusion, the ameliorative effects of AMFJ in the experimental TNBSinduced colitis might be the result of its potent antioxidant and antiinflammatory properties. Copyright © 2018 Elsevier Ltd. All rights reserved.

Effect of Aronia melanocarpa fruit juice on amiodarone-induced pneumotoxicity in rats.

PubMed

Valcheva-Kuzmanova, Stefka; Stavreva, Galya; Dancheva, Violeta; Terziev, Ljudmil; Atanasova, Milena; Stoyanova, Angelina; Dimitrova, Anelia; Shopova, Veneta

2014-04-01

The fruits of Aronia melanocarpa (Michx.) Elliot is extremely rich in biologically active polyphenols. We studied the protective effect of A. melanocarpa fruit juice (AMFJ) in a model of amiodarone (AD)-induced pneumotoxicity in rats. AD was instilled intratracheally on days 0 and 2 (6.25 mg/kg). AMFJ (5 mL/kg and 10 mL/kg) was given orally from day 1 to days 2, 4, 9, and 10 to rats, which were sacrificed respectively on days 3, 5, 10, and 28 when biochemical, cytological, and immunological assays were performed. AMFJ antagonized AD-induced increase of the lung weight coefficient. In bronchoalveolar lavage fluid, AD increased significantly the protein content, total cell count, polymorphonuclear cells, lymphocytes and the activity of lactate dehydrogenase, acid phosphatase and alkaline phosphatase on days 3 and 5. In AMFJ-treated rats these indices of direct toxic damage did not differ significantly from the control values. In lung tissue, AD induced oxidative stress measured by malondialdehyde content and fibrosis assessed by the hydroxyproline level. AMFJ prevented these effects of AD. In rat serum, AD caused a significant elevation of interleukin IL-6 on days 3 and 5, and a decrease of IL-10 on day 3. In AMFJ-treated rats, these indices of inflammation had values that did not differ significantly from the control ones. AMFJ could have a protective effect against AD-induced pulmonary toxicity as evidenced by the reduced signs of AD-induced direct toxic damage, oxidative stress, inflammation, and fibrosis.

Aronia melanocarpa Concentrate Ameliorates Pro-Inflammatory Responses in HaCaT Keratinocytes and 12-O-Tetradecanoylphorbol-13-Acetate-Induced Ear Edema in Mice.

PubMed

Goh, Ah Ra; Youn, Gi Soo; Yoo, Ki-Yeon; Won, Moo Ho; Han, Sang-Zin; Lim, Soon Sung; Lee, Keun Wook; Choi, Soo Young; Park, Jinseu

2016-07-01

Abnormal expression of pro-inflammatory mediators such as cell adhesion molecules and cytokines has been implicated in various inflammatory skin diseases, including atopic dermatitis. In this study, we investigated the anti-inflammatory activity of Aronia melanocarpa concentrate (AC) and its action mechanisms using in vivo and in vitro skin inflammation models. Topical application of AC on mouse ears significantly suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema formation, as judged by measuring ear thickness and weight, and histological analysis. Topical administration of AC also reduced the expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in TPA-stimulated mouse ears. Pretreatment with AC suppressed TNF-α-induced ICAM-I expression and subsequent monocyte adhesiveness in human keratinocyte cell line HaCaT. In addition, AC significantly decreased intracellular reactive oxygen species (ROS) generation as well as mitogen-activated protein kinase (MAPK) activation in TNF-α-stimulated HaCaT cells. AC and its constituent cyanidin 3-glucoside also attenuated TNF-α-induced IKK activation, IκB degradation, p65 phosphorylation/nuclear translocation, and p65 DNA binding activity in HaCaT cells. Overall, our results indicate that AC exerts anti-inflammatory activities by inhibiting expression of pro-inflammatory mediators in vitro and in vivo possibly through suppression of ROS-MAPK-NF-κB signaling pathways. Therefore, AC may be developed as a therapeutic agent to treat various inflammatory skin diseases.

Changes of platelet antioxidative enzymes during oxidative stress: the protective effect of polyphenol-rich extract from berries of Aronia melanocarpa and grape seeds.

PubMed

Kedzierska, Magdalena; Olas, Beata; Wachowicz, Barbara; Stochmal, Anna; Oleszek, Wiesław; Erler, Joachim

2011-01-01

Aronia melanocarpa fruits (Rosaceae) and grape seeds (seeds of Vitis vinifera, Vitaceae) are two of the richest plant sources of phenolic substances, and they have been shown to have various biological activities. The aim of the present study was to investigate and compare the action of phenolic extracts (at concentrations 5-100 µg/mL) of two different plants, berries of A. melanocarpa (chokebbery) and grape seeds, on the activities of various antioxidative enzymes, the amount of glutathione (as an important component of redox status) in control the platelets and platelets treated with H(2)O(2) (the strong physiological oxidant) in vitro. The properties of these two tested extracts were also compared with the action of a well characterized antioxidative and antiplatelet commercial monomeric polyphenol - resveratrol. The extract from berries of A. melanocarpa, like the extract from grape seeds, reduced the changes in activities of different antioxidative enzymes (glutathione peroxidase, superoxide dismutase, and catalase) in platelets treated with H(2)O(2). The action of the two tested plant extracts and H(2)O(2) evoked a significant increase of reduced glutathione in platelets compared with platelets treated with H(2)O(2) only. Comparative studies indicate that the two tested plant extracts had similar antioxidative properties, and were found to be more reactive in blood platelets than the solution of resveratrol.

Polyphenols from Berries of Aronia melanocarpa Reduce the Plasma Lipid Peroxidation Induced by Ziprasidone

PubMed Central

Dietrich-Muszalska, Anna; Kopka, Justyna

2014-01-01

Background. Oxidative stress in schizophrenia may be caused partially by the treatment of patients with antipsychotics. The aim of the study was to establish the effects of polyphenol compounds derived from berries of Aronia melanocarpa (Aronox) on the plasma lipid peroxidation induced by ziprasidone in vitro. Methods. Lipid peroxidation was measured by the level of thiobarbituric acid reactive species (TBARS). The samples of plasma from healthy subjects were incubated with ziprasidone (40 ng/ml; 139 ng/ml; and 250 ng/ml) alone and with Aronox (5 ug/ml; 50 ug/ml). Results. We observed a statistically significant increase of TBARS level after incubation of plasma with ziprasidone (40 ng/ml; 139 ng/ml; and 250 ng/ml) (after 24 h incubation: P = 7.0 × 10−4, P = 1.6 × 10−3, and P = 2.7 × 10−3, resp.) and Aronox lipid peroxidation caused by ziprasidone was significantly reduced. After 24-hour incubation of plasma with ziprasidone (40 ng/ml; 139 ng/ml; and 250 ng/ml) in the presence of 50 ug/ml Aronox, the level of TBARS was significantly decreased: P = 6.5 × 10−8, P = 7.0 × 10−6, and P = 3.0 × 10−5, respectively. Conclusion. Aronox causes a distinct reduction of lipid peroxidation induced by ziprasidone. PMID:25061527

Antioxidants as a Potential Preventive and Therapeutic Strategy for Cadmium.

PubMed

Brzóska, Malgorzata M; Borowska, Sylwia; Tomczyk, Michal

2016-01-01

Epidemiological studies provide a growing number of evidences that chronic exposure to relatively low levels of cadmium (Cd), nowadays taking place in industrialized countries, may cause health hazard. Thus, growing interest has been focused on effective ways of protection from adverse effects of exposure to this heavy metal. Because numerous effects to Cd's toxic action result from its prooxidative properties, it seems reasonable that special attention should be directed to agents that can prevent or reduce this metal-induced oxidative stress and its consequences in tissues, organs and systems at risk of toxicity, including liver, kidneys, testes, ears, eyes, cardiovascular system and nervous system as well as bone tissue. This review discusses a wide range of natural (plant and animal origin) and synthetic antioxidants together with many plant extracts (e.g. black and green tea, Aronia melanocarpa, Allium sativum, Allium cepa, Ocimum sanctum, Phoenix dactylifera, Physalis peruviana, Zingiber officinale) that have been shown to prevent from Cd toxicity. Moreover, some attention has been focused on the fact that substances not possessing antioxidative potential may also prevent Cd-induced oxidative stress and its consequences. So far, most of the data on the protective effects of the natural and synthetic antioxidants and plant extracts come from studies in animals' models; however, numerous of them seem to be promising preventive/therapeutic strategies for Cd toxicity in humans. Further investigation of prophylactic and therapeutic use of antioxidants in populations exposed to Cd environmentally and occupationally is warranted, given that therapeutically effective chelation therapy for this toxic metal is currently lacking.

Protective Effect of Aronia Melanocarpa Polyphenols on Cadmium Accumulation in the Body: A Study in a Rat Model of Human Exposure to this Metal.

PubMed

Brzoska, Malgorzata M; Galazyn-Sidorczuk, Malgorzata; Jurczuk, Maria; Tomczyk, Michal

2015-01-01

Recently a growing attention has been paid to the possibility of using biologically active compounds, including polyphenols, for the prevention of unfavourable effects of exposure to xenobiotics. The study was aimed to investigate, in a female rat model, whether consumption of Aronia melanocarpa polyphenols (AMP) under chronic exposure to cadmium (Cd) decreases the gastrointestinal absorption and body burden of this heavy metal. For this purpose, Cd turnover (apparent absorption, retention in the body, concentration in the blood, soft tissues and bone tissue, total pool in internal organs, faecal and urinary excretion) was evaluated in the female Wistar rats who were administered only a 0.1% aqueous extract of AMP (prepared from the powdered extract containing 65.74% of polyphenols) as drinking fluid or/and Cd in diet (1 and 5 mg/kg) for up to 24 months. AMP administration under the low Cd treatment (1 mg/kg diet) had only a very slight protective impact against this metal accumulation in the organism, whereas polyphenols application under moderate exposure (5 mg Cd/kg diet) significantly decreased apparent absorption and retention in the body, and increased urinary concentration of this xenobiotic, resulting in its lower concentration in the blood and lower accumulation in soft tissues (mainly in the liver and kidneys) and bone tissue. Based on the study, it can be concluded that consumption of polyphenol- rich products may prevent Cd absorption from the diet polluted by this metal and its accumulation in the females' body, and thus also prevent its toxic action.

Effect of Aronia melanocarpa fruit juice on amiodarone-induced pneumotoxicity in rats

PubMed Central

Valcheva-Kuzmanova, Stefka; Stavreva, Galya; Dancheva, Violeta; Terziev, Ljudmil; Atanasova, Milena; Stoyanova, Angelina; Dimitrova, Anelia; Shopova, Veneta

2014-01-01

Background: The fruits of Aronia melanocarpa (Michx.) Elliot is extremely rich in biologically active polyphenols. Objective: We studied the protective effect of A. melanocarpa fruit juice (AMFJ) in a model of amiodarone (AD)-induced pneumotoxicity in rats. Materials and Methods: AD was instilled intratracheally on days 0 and 2 (6.25 mg/kg). AMFJ (5 mL/kg and 10 mL/kg) was given orally from day 1 to days 2, 4, 9, and 10 to rats, which were sacrificed respectively on days 3, 5, 10, and 28 when biochemical, cytological, and immunological assays were performed. Results: AMFJ antagonized AD-induced increase of the lung weight coefficient. In bronchoalveolar lavage fluid, AD increased significantly the protein content, total cell count, polymorphonuclear cells, lymphocytes and the activity of lactate dehydrogenase, acid phosphatase and alkaline phosphatase on days 3 and 5. In AMFJ-treated rats these indices of direct toxic damage did not differ significantly from the control values. In lung tissue, AD induced oxidative stress measured by malondialdehyde content and fibrosis assessed by the hydroxyproline level. AMFJ prevented these effects of AD. In rat serum, AD caused a significant elevation of interleukin IL-6 on days 3 and 5, and a decrease of IL-10 on day 3. In AMFJ-treated rats, these indices of inflammation had values that did not differ significantly from the control ones. Conclusion: AMFJ could have a protective effect against AD-induced pulmonary toxicity as evidenced by the reduced signs of AD-induced direct toxic damage, oxidative stress, inflammation, and fibrosis. PMID:24914278

Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation

PubMed Central

Kim, Jung-Hee; Lee, Eun Byul; Hur, Wonhee; Kwon, Oh-Joo; Park, Hyoung-Jin; Yoon, Seung Kew

2017-01-01

Nonalcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome. Studies have demonstrated that anthocyanin-rich foods may improve hyperlipidemia and ameliorate hepatic steatosis. Here, effects of Aronia melanocarpa (AM), known to be rich of anthocyanins, on hepatic lipid metabolism and adipogenic genes were determined. AM was treated to C57BL/6N mice fed with high fat diet (HFD) or to FL83B cells treated with free fatty acid (FFA). Changes in levels of lipids, enzymes and hormones were observed, and expressions of adipogenic genes involved in hepatic lipid metabolism were detected by PCR, Western blotting and luciferase assay. In mice, AM significantly reduced the body and liver weight, lipid accumulation in the liver, and levels of biochemical markers such as fatty acid synthase, hepatic triglyceride and leptin. Serum transaminases, indicators for hepatocyte injury, were also suppressed, while superoxide dismutase activity and liver antioxidant capacity were significantly increased. In FL83B cells, AM significantly reduced FFA-induced lipid droplet accumulation. Protein synthesis of an adipogenic transcription factor, peroxisome proliferator-activated receptor γ2 (PPARγ2) was inhibited in vivo. Furthermore, transcriptional activity of PPARγ2 was down-regulated in vitro, and mRNA expression of PPARγ2 and its downstream target genes, adipocyte protein 2 and lipoprotein lipase were down-regulated by AM both in vitro and in vivo. These results show beneficial effects of AM against hepatic lipid accumulation through the inhibition of PPARγ2 expression along with improvements in body weight, liver functions, lipid profiles and antioxidant capacity suggesting the potential therapeutic efficacy of AM on NAFLD. PMID:28081181

Modeling and optimization of ultrasound-assisted extraction of polyphenolic compounds from Aronia melanocarpa by-products from filter-tea factory.

PubMed

Ramić, Milica; Vidović, Senka; Zeković, Zoran; Vladić, Jelena; Cvejin, Aleksandra; Pavlić, Branimir

2015-03-01

Aronia melanocarpa by-product from filter-tea factory was used for the preparation of extracts with high content of bioactive compounds. Extraction process was accelerated using sonication. Three level, three variable face-centered cubic experimental design (FCD) with response surface methodology (RSM) was used for optimization of extraction in terms of maximized yields for total phenolics (TP), flavonoids (TF), anthocyanins (MA) and proanthocyanidins (TPA) contents. Ultrasonic power (X₁: 72-216 W), temperature (X₂: 30-70 °C) and extraction time (X₃: 30-90 min) were investigated as independent variables. Experimental results were fitted to a second-order polynomial model where multiple regression analysis and analysis of variance were used to determine fitness of the model and optimal conditions for investigated responses. Three-dimensional surface plots were generated from the mathematical models. The optimal conditions for ultrasound-assisted extraction of TP, TF, MA and TPA were: X₁=206.64 W, X₂=70 °C, X₃=80.1 min; X₁=210.24 W, X₂=70 °C, X₃=75 min; X₁=216 W, X₂=70 °C, X₃=45.6 min and X₁=199.44 W, X₂=70 °C, X₃=89.7 min, respectively. Generated model predicted values of the TP, TF, MA and TPA to be 15.41 mg GAE/ml, 9.86 mg CE/ml, 2.26 mg C3G/ml and 20.67 mg CE/ml, respectively. Experimental validation was performed and close agreement between experimental and predicted values was found (within 95% confidence interval). Copyright © 2014 Elsevier B.V. All rights reserved.

Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation.

PubMed

Park, Chung-Hwa; Kim, Jung-Hee; Lee, Eun Byul; Hur, Wonhee; Kwon, Oh-Joo; Park, Hyoung-Jin; Yoon, Seung Kew

2017-01-01

Nonalcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome. Studies have demonstrated that anthocyanin-rich foods may improve hyperlipidemia and ameliorate hepatic steatosis. Here, effects of Aronia melanocarpa (AM), known to be rich of anthocyanins, on hepatic lipid metabolism and adipogenic genes were determined. AM was treated to C57BL/6N mice fed with high fat diet (HFD) or to FL83B cells treated with free fatty acid (FFA). Changes in levels of lipids, enzymes and hormones were observed, and expressions of adipogenic genes involved in hepatic lipid metabolism were detected by PCR, Western blotting and luciferase assay. In mice, AM significantly reduced the body and liver weight, lipid accumulation in the liver, and levels of biochemical markers such as fatty acid synthase, hepatic triglyceride and leptin. Serum transaminases, indicators for hepatocyte injury, were also suppressed, while superoxide dismutase activity and liver antioxidant capacity were significantly increased. In FL83B cells, AM significantly reduced FFA-induced lipid droplet accumulation. Protein synthesis of an adipogenic transcription factor, peroxisome proliferator-activated receptor γ2 (PPARγ2) was inhibited in vivo. Furthermore, transcriptional activity of PPARγ2 was down-regulated in vitro, and mRNA expression of PPARγ2 and its downstream target genes, adipocyte protein 2 and lipoprotein lipase were down-regulated by AM both in vitro and in vivo. These results show beneficial effects of AM against hepatic lipid accumulation through the inhibition of PPARγ2 expression along with improvements in body weight, liver functions, lipid profiles and antioxidant capacity suggesting the potential therapeutic efficacy of AM on NAFLD.

Short-term supplementation with Aronia melanocarpa extract improves platelet aggregation, clotting, and fibrinolysis in patients with metabolic syndrome.

PubMed

Sikora, Joanna; Broncel, Marlena; Markowicz, Magdalena; Chałubiński, Maciej; Wojdan, Katarzyna; Mikiciuk-Olasik, Elżbieta

2012-08-01

A diet rich in berries is believed to play a distinct role in the prevention of metabolic diseases associated with obesity. So far, there have been no published clinical observations evaluating the influence of Aronia melanocarpa on hemostasis. The aim of our study was to investigate the effects of A. melanocarpa extract (AM) supplementation on platelet aggregation, clot formation, and lysis in patients with metabolic syndrome (MS). Middle-aged non-medicated subjects with MS (n = 38) and 14 healthy volunteers were included in this study. Patients with MS were treated with 100 mg of AM three times daily for 2 months. We observed a significant reduction in the concentration of TC, LDL-C, and TG after AM supplementation. Beneficial changes in coagulation parameters were also observed. After 1 month of AM administration, we noticed significant inhibition of platelet aggregation. However, this effect became less pronounced after 2 months of supplementation. In the case of coagulation induced by endogenic thrombin, a significant decrease in the overall potential for coagulation was induced after 1 or 2 months of supplementation. Moreover, after 1 month of AM extract supplementation, we observed a beneficial reduction in the overall potential for clot formation and fibrinolysis. We observed the normalization of hemostasis parameters in MS patients after both 1 and 2 months of AM administration. After 1 month of AM supplementation, we found favorable changes in regards to the overall potential for plasma clotting, clot formation, and lysis, as well as in the lipid profiles of subjects.

The use of fruit extracts for production of apple chips with enhanced antioxidant activity

PubMed

Tarko, Tomasz; Duda-Chodak, Aleksandra; Semik-Szczurak, Dorota

Style and pace of life make consumers more willing to reach for snack products. This group of processed food includes, among others, fruit chips. Due to the increasing incidence of diseases associated with the excessive exposure to free radicals foods enriched with antioxidant compounds, eg. polyphenols, can be introduced into the sale. The aim of the study was to use the fruit extracts for the production of apple chips with enhanced antioxidant activity. ‘Golden Delicious’ variety of apple fruit was used to produce chips. Apple chips were prepared by slicing, soaking in a sugar solution and pre-drying in a microwave oven. Chips were enriched with extracts prepared from fruits of chokeberry, five-flavor berry, Cornelian cherry, woodland hawthorn, goji berry, Japanese quince and cranberry microcarpa. For this purpose, pre-dried apple slices were soaked (5 min) in ethanolic extract of fruits and then dried to achieve a 5% moisture content. Chips were sensory evaluated and their antioxidant activity and total polyphenols content were determined. All enriched apple chips were characterized by high antioxidant activity and a relatively high value of total polyphenols content. Chips soaked in extracts of five-flavor berry, cranberry and goji berry were characterized by the highest antioxidant potential. Samples obtained by using chokeberry and Cornelian cherry extracts showed the highest content of polyphenols. High sensory attractiveness of enriched chips was also showed. The chips with the addition of fiveflavor berry extract were exceptions. Their taste was not acceptable. Fruit extracts are a valuable material for chips enrichment. Taking into account all the analyzed differentiators, extracts of Japanese quince, goji berry and woodland hawthorn were found to be the best enriching additives. The chips soaked in extract of five-flavor berry, despite their high antioxidant activity, were disqualified due to very low score of sensory evaluation.

Arbutin production via biotransformation of hydroquinone in in vitro cultures of Aronia melanocarpa (Michx.) Elliott.

PubMed

Kwiecień, Inga; Szopa, Agnieszka; Madej, Kornelia; Ekiert, Halina

2013-01-01

Arbutin (hydroquinone β-D-glucoside) is a compound of plant origin possessing valuable therapeutic (urinary tract disinfection) and cosmetic (skin whitening) properties, which can be obtained from in vitro cultures of plants belonging to different taxa via biotransformation of exogenously supplemented hydroquinone. Agitating cultures of Aronia melanocarpa were maintained on the Murashige and Skoog medium containing growth regulators: the cytokinin - BAP (6-benzylaminopurine), 2 mg/l and the auxin NAA (α-naphthaleneacetic acid), 2 mg/l. The biomass was cultured for 2 weeks and then hydroquinone was supplemented at the following doses: 96, 144, 192, 288 and 384 mg/l either undivided or divided into two or three portions added at 24-hour intervals. The content of the reaction product - arbutin, was determined using an HPLC method in methanolic extracts from biomass and lyophilized medium samples collected 24 hours after the addition of the last precursor dose. The total amounts of arbutin were very diverse, from 2.71 to 8.27 g/100g d.w. The production of arbutin rose with increasing hydroquinone concentration. The maximum content of the product was observed after hydroquinone addition at 384 mg/l divided into two portions. Biotransformation efficiency also varied widely, ranging from 37.04% do 73.80%. The identity of the product - arbutin, after its isolation and purification was confirmed by spectral analysis ((1)H-NMR spectrum). The maximum amount of arbutin obtained was higher than that required by the latest 9(th) Edition of the Polish Pharmacopoeia and by the newest 8th Edithion of European Pharmacopoeia for Uvae ursi folium (7.0 g/100g d.w.), and is interesting from practical point of view.

Effects of polyphenol-rich extract from berries of Aronia melanocarpa on the markers of oxidative stress and blood platelet activation.

PubMed

Olas, Beata; Kedzierska, Magdalena; Wachowicz, Barbara; Stochmal, Anna; Oleszek, Wieslaw

2010-01-01

Bioactive substances found in numerous foods can be successfully and safely used to modify various cellular functions and affect the oxidative stress. Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances shown to have anti-inflammatory, antitumor, antioxidative and antiplatelet activities. We investigated antioxidant properties of the extract from berries of A. melanocarpa by the estimation of the selected and other biomarkers of oxidative stress, i.e. the level of 8-epi-prostaglandin F(2) (8-EPI) (by immunoassay kit) and the amount of glutathione (by HPLC method) in control platelets and platelets treated with H(2)O(2). The expression of alpha(IIb)beta(3) (a marker of platelet activation) was measured by flow cytometer. The antioxidative and antiplatelet properties of the tested extract were also compared with the action of a well characterized antioxidative and antiplatelet commercial monomeric polyphenol-resveratrol. The extract from berries of A. melanocarpa (at the highest tested concentration -100 microg/ml) decreased the production of 8-EPI (a marker of lipid peroxidation) in control blood platelets and platelets treated with H(2)O(2) (2 mM). A combined action of the tested plant extract and H(2)O(2) evoked a significant increase of reduced form of glutathione in platelets compared with cells treated with H(2)O(2) only. Moreover, the tested plant extract (at the highest used concentration -100 microg/ml) reduced the expression of alpha(IIb)beta(3) on blood platelets. Comparative studies indicate that the tested plant extract was found to be more reactive in blood platelets than the solution of pure resveratrol.

Aronia melanocarpa juice induces a redox-sensitive p73-related caspase 3-dependent apoptosis in human leukemia cells.

PubMed

Sharif, Tanveer; Alhosin, Mahmoud; Auger, Cyril; Minker, Carole; Kim, Jong-Hun; Etienne-Selloum, Nelly; Bories, Pierre; Gronemeyer, Hinrich; Lobstein, Annelise; Bronner, Christian; Fuhrmann, Guy; Schini-Kerth, Valérie B

2012-01-01

Polyphenols are natural compounds widely present in fruits and vegetables, which have antimutagenic and anticancer properties. The aim of the present study was to determine the anticancer effect of a polyphenol-rich Aronia melanocarpa juice (AMJ) containing 7.15 g/L of polyphenols in the acute lymphoblastic leukemia Jurkat cell line, and, if so, to clarify the underlying mechanism and to identify the active polyphenols involved. AMJ inhibited cell proliferation, which was associated with cell cycle arrest in G(2)/M phase, and caused the induction of apoptosis. These effects were associated with an upregulation of the expression of tumor suppressor p73 and active caspase 3, and a downregulation of the expression of cyclin B1 and the epigenetic integrator UHRF1. AMJ significantly increased the formation of reactive oxygen species (ROS), decreased the mitochondrial membrane potential and caused the release of cytochrome c into the cytoplasm. Treatment with intracellular ROS scavengers prevented the AMJ-induced apoptosis and upregulation of the expression of p73 and active caspase 3. The fractionation of the AMJ and the use of identified isolated compounds indicated that the anticancer activity was associated predominantly with chlorogenic acids, some cyanidin glycosides, and derivatives of quercetin. AMJ treatment also induced apoptosis of different human lymphoblastic leukemia cells (HSB-2, Molt-4 and CCRF-CEM). In addition, AMJ exerted a strong pro-apoptotic effect in human primary lymphoblastic leukemia cells but not in human normal primary T-lymphocytes. Thus, the present findings indicate that AMJ exhibits strong anticancer activity through a redox-sensitive mechanism in the p53-deficient Jurkat cells and that this effect involves several types of polyphenols. They further suggest that AMJ has chemotherapeutic properties against acute lymphoblastic leukemia by selectively targeting lymphoblast-derived tumor cells.

Berries: emerging impact on cardiovascular health

PubMed Central

Basu, Arpita; Rhone, Michael; Lyons, Timothy J

2011-01-01

Berries are a good source of polyphenols, especially anthocyanins, micronutrients, and fiber. In epidemiological and clinical studies, these constituents have been associated with improved cardiovascular risk profiles. Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (either fresh, or as juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism. Benefits were seen in healthy subjects and in those with existing metabolic risk factors. Underlying mechanisms for these beneficial effects are believed to include upregulation of endothelial nitric oxide synthase, decreased activities of carbohydrate digestive enzymes, decreased oxidative stress, and inhibition of inflammatory gene expression and foam cell formation. Though limited, these data support the recommendation of berries as an essential fruit group in a heart-healthy diet. PMID:20384847

Aronia melanocarpa Juice Induces a Redox-Sensitive p73-Related Caspase 3-Dependent Apoptosis in Human Leukemia Cells

PubMed Central

Sharif, Tanveer; Alhosin, Mahmoud; Auger, Cyril; Minker, Carole; Kim, Jong-Hun; Etienne-Selloum, Nelly; Bories, Pierre; Gronemeyer, Hinrich; Lobstein, Annelise; Bronner, Christian; Fuhrmann, Guy; Schini-Kerth, Valérie B.

2012-01-01

Polyphenols are natural compounds widely present in fruits and vegetables, which have antimutagenic and anticancer properties. The aim of the present study was to determine the anticancer effect of a polyphenol-rich Aronia melanocarpa juice (AMJ) containing 7.15 g/L of polyphenols in the acute lymphoblastic leukemia Jurkat cell line, and, if so, to clarify the underlying mechanism and to identify the active polyphenols involved. AMJ inhibited cell proliferation, which was associated with cell cycle arrest in G2/M phase, and caused the induction of apoptosis. These effects were associated with an upregulation of the expression of tumor suppressor p73 and active caspase 3, and a downregulation of the expression of cyclin B1 and the epigenetic integrator UHRF1. AMJ significantly increased the formation of reactive oxygen species (ROS), decreased the mitochondrial membrane potential and caused the release of cytochrome c into the cytoplasm. Treatment with intracellular ROS scavengers prevented the AMJ-induced apoptosis and upregulation of the expression of p73 and active caspase 3. The fractionation of the AMJ and the use of identified isolated compounds indicated that the anticancer activity was associated predominantly with chlorogenic acids, some cyanidin glycosides, and derivatives of quercetin. AMJ treatment also induced apoptosis of different human lymphoblastic leukemia cells (HSB-2, Molt-4 and CCRF-CEM). In addition, AMJ exerted a strong pro-apoptotic effect in human primary lymphoblastic leukemia cells but not in human normal primary T-lymphocytes. Thus, the present findings indicate that AMJ exhibits strong anticancer activity through a redox-sensitive mechanism in the p53-deficient Jurkat cells and that this effect involves several types of polyphenols. They further suggest that AMJ has chemotherapeutic properties against acute lymphoblastic leukemia by selectively targeting lymphoblast-derived tumor cells. PMID:22412883

The nitrative and oxidative stress in blood platelets isolated from breast cancer patients: the protectory action of aronia melanocarpa extract.

PubMed

Kedzierska, Magdalena; Olas, Beata; Wachowicz, Barbara; Stochmal, Anna; Oleszek, Wieslaw; Jeziorski, Arkadiusz; Piekarski, Janusz

2010-01-01

Since mechanisms involved in the relationship between oxidative stress and breast cancer are still unclear, the aim of our present study was to evaluate oxidative/nitrative modifications of blood platelet proteins by measuring the level of biomarkers of oxidative/nitrative stress such as carbonyl groups, thiol groups and 3-nitrotyrosine in proteins in patients with benign breast diseases and in patients with invasive breast cancer, and compare with the control group. Levels of carbonyl groups and 3-nitrotyrosine residues in platelet proteins were measured by ELISA and a competition ELISA, respectively. The method with 5,5′-dithio-bis(2-nitro-benzoic acid) has been used to analyse free thiol groups in platelet proteins. Patients were hospitalized in the Department of Oncological Surgery, Medical University of Lodz, Poland. Exogenous antioxidants reduce oxidative stress, therefore we also investigated in a model system in vitro the effects of a polyphenol rich extract of Aronia melanocarpa (Rosaceae, final concentration of 50 µg/ml, 5 min, 37°C) on modified blood platelet proteins as well from patients with breast cancer and from the healthy group. We demonstrated in platelet proteins from patients with invasive breast cancer a higher level of carbonyl groups than in the control healthy group (p < 0.02). The level of 3-nitrotyrosine in platelet proteins from patients with invasive breast cancer was also significantly higher than in the healthy subject group (p < 0.001). In contrast, the amount of thiol groups in platelet proteins from patients was significantly lower (about < 50%) than in control blood platelets. In a model system in vitro we also observed that the extract from berries of A. melanocarpa (50 µg/ml, 5 min, 37°C) due to antioxidant action, significantly reduced the oxidative/nitrative stress (measured by thiol groups and 3-nitrotyrosine) in platelets, not only from the healthy group, but also from patients with benign breast diseases and in patients with invasive breast cancer.

Effects of Aronia melanocarpa Fruit Juice on Isolated Rat Hepatocytes.

PubMed

Kondeva-Burdina, Magdalena; Valcheva-Kuzmanova, Stefka; Markova, Tsvetelina; Mitcheva, Mitka; Belcheva, Anna

2015-10-01

Aronia melanocarpa (Michx.) Elliot fruits are very rich in polyphenols - procyanidins, flavonoids, and phenolic acids. On rat hepatocytes, isolated by two-stepped collagenase perfusion, we investigated the effect of A. melanocarpa fruit juice (AMFJ) in two models of liver toxicity caused by (i) metabolic bioactivation of carbon tetrachloride (CCl4), and (ii) tert-butyl hydroperoxide (t-BuOOH)-induced oxidative stress. Isolated rat hepatocytes are a suitable model for hepatotoxicity studies. We determined the main parameters of the functional and metabolic status of rat hepatocytes: Cell viability (measured by trypan blue exclusion) and the levels of lactate dehydrogenase (LDH), reduced glutathione (GSH), and malondialdehyde (MDA). These parameters were used to investigate the protective effects of AMFJ in the two toxicity models. The effects of AMFJ were compared with those of silymarin. The cells were treated either with AMFJ or silymarin at increasing concentrations of 5 μg/ml, 10 μg/ml, 30 μg/ml, 50 μg/ml, and 100 μg/ml which were used for measuring of IC50. In both toxicity models - CCl4 and t-BuOOH, AMFJ showed statistically significant cytoprotective and antioxidant activities. AMFJ prevented the loss of cell viability and GSH depletion, decreased LDH leakage and MDA production. The effects of AMFJ at the concentrations of 5, 10, 30, and 50 μg/ml were similar to those of the same concentrations of silymarin, while the effect of the highest AMFJ concentration of 100 μg/ml was higher than that of the same silymarin concentration. The effects were concentration-dependent and more prominent in the t-BuOOH model, compared to those in the CCl4 model. The cytoprotective and antioxidant effects of AMFJ established in this study might be due to its polyphenolic ingredients, which could influence the cytochrome P450-mediated metabolism of the experimental hepatotoxic substances (CCl4 and t-BuOOH) and could act as free radical scavengers. The stronger effects of the highest AMFJ concentration in comparison with that of silymarin were possibly due to the combined presence of different polyphenols in the juice. On rat hepatocytes, isolated by two-stepped collagenase perfusion, we investigated the effect of Aronia melanocarpa fruit juice (AMFJ) in two models of liver toxicity caused by i) metabolic bioactivation of carbon tetrachloride (CCl4), and ii) tert-butyl hydroperoxide (t-BuOOH)-induced oxidative stress. In both toxicity models - CCl4 and t-BuOOH, AMFJ showed statistically significant cytoprotective and antioxidant activities. AMFJ prevented the loss of cell viability and GSH depletion, decreased LDH leakage and MDA production. The effects of AMFJ at the concentrations of 5, 10, 30, and 50 μg/ml were similar to those of the same concentrations of silymarin, while the effect of the highest AMFJ concentration of 100 μg/ml was higher than that of the same silymarin concentration. The effects were concentration-dependent and were more prominent in the t-BuOOH model, compared to those in the CCl4 model.

Effects of Aronia melanocarpa Fruit Juice on Isolated Rat Hepatocytes

PubMed Central

Kondeva-Burdina, Magdalena; Valcheva-Kuzmanova, Stefka; Markova, Tsvetelina; Mitcheva, Mitka; Belcheva, Anna

2015-01-01

Background: Aronia melanocarpa (Michx.) Elliot fruits are very rich in polyphenols – procyanidins, flavonoids, and phenolic acids. Objective: On rat hepatocytes, isolated by two-stepped collagenase perfusion, we investigated the effect of A. melanocarpa fruit juice (AMFJ) in two models of liver toxicity caused by (i) metabolic bioactivation of carbon tetrachloride (CCl4), and (ii) tert-butyl hydroperoxide (t-BuOOH)-induced oxidative stress. Materials and Methods: Isolated rat hepatocytes are a suitable model for hepatotoxicity studies. We determined the main parameters of the functional and metabolic status of rat hepatocytes: Cell viability (measured by trypan blue exclusion) and the levels of lactate dehydrogenase (LDH), reduced glutathione (GSH), and malondialdehyde (MDA). These parameters were used to investigate the protective effects of AMFJ in the two toxicity models. The effects of AMFJ were compared with those of silymarin. The cells were treated either with AMFJ or silymarin at increasing concentrations of 5 μg/ml, 10 μg/ml, 30 μg/ml, 50 μg/ml, and 100 μg/ml which were used for measuring of IC50. Results: In both toxicity models – CCl4 and t-BuOOH, AMFJ showed statistically significant cytoprotective and antioxidant activities. AMFJ prevented the loss of cell viability and GSH depletion, decreased LDH leakage and MDA production. The effects of AMFJ at the concentrations of 5, 10, 30, and 50 μg/ml were similar to those of the same concentrations of silymarin, while the effect of the highest AMFJ concentration of 100 μg/ml was higher than that of the same silymarin concentration. The effects were concentration-dependent and more prominent in the t-BuOOH model, compared to those in the CCl4 model. Conclusion: The cytoprotective and antioxidant effects of AMFJ established in this study might be due to its polyphenolic ingredients, which could influence the cytochrome P450-mediated metabolism of the experimental hepatotoxic substances (CCl4 and t-BuOOH) and could act as free radical scavengers. The stronger effects of the highest AMFJ concentration in comparison with that of silymarin were possibly due to the combined presence of different polyphenols in the juice. SUMMARY On rat hepatocytes, isolated by two-stepped collagenase perfusion, we investigated the effect of Aronia melanocarpa fruit juice (AMFJ) in two models of liver toxicity caused by i) metabolic bioactivation of carbon tetrachloride (CCl4), and ii) tert-butyl hydroperoxide (t-BuOOH)-induced oxidative stress. In both toxicity models – CCl4 and t-BuOOH, AMFJ showed statistically significant cytoprotective and antioxidant activities. AMFJ prevented the loss of cell viability and GSH depletion, decreased LDH leakage and MDA production. The effects of AMFJ at the concentrations of 5, 10, 30, and 50 μg/ml were similar to those of the same concentrations of silymarin, while the effect of the highest AMFJ concentration of 100 μg/ml was higher than that of the same silymarin concentration. The effects were concentration-dependent and were more prominent in the t-BuOOH model, compared to those in the CCl4 model. PMID:27013800

Modeling of process of lifting power change of baker's yeast pressed depending on nature and quantity of introduced vegetable component

NASA Astrophysics Data System (ADS)

Belokurov, S. V.; Rodionova, N. S.; Belokurova, E. V.; Alexeeva, T. V.

2018-05-01

The work presents data on the effect of non-traditional powdered semi-finished products of plant origin: chokeberry, walnut partitions and sea buckthorn berries on the lifting power of baker's yeast. Various amounts of powdered semi-finished products of plant origin are introduced into the dough, directly at the stage of introducing the main components of the formulation, replacing them with some of the wheat flour. Studies have shown that the addition of small amounts of unconventional powdered plant-based semi-finished products (1 ... 5%) makes it possible to correct the lifting power of baking yeast, which, in consequence, affects the quality indicators of finished products. The paper presents a mathematical model of the change in the lift strength of baker's yeast, depending on the nature and amount of the powdered semi-finished product introduced.

Bioactive β-carbolines norharman and harman in traditional and novel raw materials for chicory coffee.

PubMed

Wojtowicz, Elżbieta; Zawirska-Wojtasiak, Renata; Przygoński, Krzysztof; Mildner-Szkudlarz, Sylwia

2015-05-15

The β-carboline compounds norharman and harman exhibit neuroactive activity in the human body. Chicory coffee has proved to be a source of β-carboline compounds. This study assessed the norharman and harman contents of traditional and novel raw materials for the production of chicory coffee, as well as in samples of chicory coffee with novel additives. The highest content of the β-carbolines among the traditional raw materials was recorded in roasted sugar beet (2.26 μg/g), while roasting the chicory caused a 25-fold increase in the content of norharman in this raw material (from 0.05 to 1.25 μg/g). In novel raw materials not subjected to the action of high temperature, β-carboline was not detected. Among the roasted novel raw materials, the highest contents of harman and norharman were found in artichokes. High harman levels were also recorded in roasted chokeberry. Copyright © 2014 Elsevier Ltd. All rights reserved.

Cell dedifferentiation, callus induction and somatic embryogenesis in Crataegus spp.

PubMed

Taimori, N; Kahrizi, D; Abdossi, V; Papzan, A H

2016-09-30

The present study describes the effects of light conditions, different kinds and concentrations of auxins [Naphthylacetic acid (NAA) and dichlorophenoxyacetic acid (2,4-D)] with cytokinin (Kin) in MS medium on callus induction and embryogenesis in Crataegus pseudoheterophylla, C. aronia and C.meyeri. At first leave explants sections were cultured on different combinations of plant growth regulators in dark and light for callus initiation and light conditions to evaluation the percentage and duration of survival, callus diameter, callus fresh weight and dry. Results of effects of plant growth regulators and light conditions on callus initiation revealed that highest percentage of callus initiation leaves in treatment (0.5 mg/l 2.4-D+0.5 mg/l KIN) for species C.pseudoheterophylla in dark conditions (100%). Dark conditions (100%) were more effective on callogenesis than light conditions (Photoperiodicity of 16-h and at light intensity of 40 µmol m-2 s-1). The callus induction of in vitro (64-100%) leaves was better than the ex vitro ones (0-100%). The combination of 2,4-D and Kin of in vitro leaves callogenesis has been indicated faster (one weeks) than the other combinations. The results also showed that the highest percentage (100%) and survival duration (6 months) was found in species C. pseudoheterophylla and C. meyeri in 0.1 mg/l 2,4.D + 0.5 mg/l KIN and 0.5 mg/l 2,4.D + 0.5 mg/l Kin. The minimum survival (0%) was absorbed in species C. aronia in 1 mg/l NAA. Maximum callus (10.63 and 10.00 mm respectively) was shown in 0.1 mg/l 2,4.D + 0.5 mg/l Kin and 0.5 mg/l 2,4.D + 0.5 mg/l Kin and was not significant differences after five week among species. The results showed that the highest fresh (1081.49 mg) and dry weight (506.88 and 506.98 mg respectively) was absorbed in species C. pseudoheterophylla in 0.1 mg/l 2,4.D + 0.5 mg/l Kin and 0.5 mg/l 2,4.D + 0.5 mg/l Kin. The embryogenesis was not occurred in any plant growth regulator combinations and species. The results of this study suggested that using 2,4-D with cytokinin (Kin) would be more beneficial for callogenesis.

Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry.

PubMed

Hartmanova, Lucie; Ranc, Vaclav; Papouskova, Barbora; Bednar, Petr; Havlicek, Vladimir; Lemr, Karel

2010-06-18

Desorption electrospray ionization (DESI) mass spectrometry appears to be a useful technique applicable in different areas (e.g. analysis of pharmaceuticals, identification of biologically active compounds in tissues, imaging mass spectrometry). Its modification termed desorption nano-electrospray (nano-DESI) was tested for analysis of anthocyanins. Acidifying of samples and acidic spray liquid (methanol:water=75:25 with 0.2% HCOOH) were essential for obtaining good quality spectra. Profiles of main anthocyanins in wine samples, two vintages (2005 and 2007) of three cultivars (Alibernet, Neronet and Rubinet), were successfully acquired. They were in agreement with results of LC/MS experiments (anthocyanins isolated by solid phase extraction were separated by mu-HPLC with gradient elution and detected by ESI-MS). Nano-DESI-MS data also allowed to determine ratio of two cultivars (Neronet and Rubinet) in their mixture and to detect coloring of wine by tenturier or elderberry extract. Detection of main anthocyanins in slices of wine grape, chokeberries and elderberries or in a wine stain on cotton fabric is also presented. Copyright (c) 2010 Elsevier B.V. All rights reserved.

Phenolic compounds extracted by acidic aqueous ethanol from berries and leaves of different berry plants.

PubMed

Tian, Ye; Liimatainen, Jaana; Alanne, Aino-Liisa; Lindstedt, Anni; Liu, Pengzhan; Sinkkonen, Jari; Kallio, Heikki; Yang, Baoru

2017-04-01

Phenolic compounds of berries and leaves of thirteen various plant species were extracted with aqueous ethanol and analyzed with UPLC-DAD-ESI-MS, HPLC-DAD, and NMR. The total content of phenolics was consistently higher in leaves than in berries (25-7856 vs. 28-711mg/100g fresh weight). Sea buckthorn leaves were richest in phenolic compounds (7856mg/100g f.w.) with ellagitannins as the dominant compound class. Sea buckthorn berries contained mostly isorhamnetin glycosides, whereas quercetin glycosides were typically abundant in most samples investigated. Anthocyanins formed the dominating group of phenolics in most dark-colored berries but phenolic acid derivatives were equally abundant in saskatoon and chokeberry berries. Caffeoylquinic acids constituted 80% of the total phenolic content (1664mg/100g f.w.) in bilberry leaves. B-type procyanidins and caffeoylquinic acids were the major phenolic compounds in hawthorn and rowanberry, respectively. Use of leaves of some species with prunasin, tyramine and β-p-arbutin, may be limited in food applications. Copyright © 2016 Elsevier Ltd. All rights reserved.

Effect of Solid Biological Waste Compost on the Metabolite Profile of Brassica rapa ssp. chinensis

PubMed Central

Neugart, Susanne; Wiesner-Reinhold, Melanie; Frede, Katja; Jander, Elisabeth; Homann, Thomas; Rawel, Harshadrai M.; Schreiner, Monika; Baldermann, Susanne

2018-01-01

Large quantities of biological waste are generated at various steps within the food production chain and a great utilization potential for this solid biological waste exists apart from the current main usage for the feedstuff sector. It remains unclear how the usage of biological waste as compost modulates plant metabolites. We investigated the effect of biological waste of the processing of coffee, aronia, and hop added to soil on the plant metabolite profile by means of liquid chromatography in pak choi sprouts. Here we demonstrate that the solid biological waste composts induced specific changes in the metabolite profiles and the changes are depending on the type of the organic residues and its concentration in soil. The targeted analysis of selected plant metabolites, associated with health beneficial properties of the Brassicaceae family, revealed increased concentrations of carotenoids (up to 3.2-fold) and decreased amounts of glucosinolates (up to 4.7-fold) as well as phenolic compounds (up to 1.5-fold). PMID:29616051

Dietary polyphenols increase fecal mucin and immunoglobulin A and ameliorate the disturbance in gut microbiota caused by a high fat diet.

PubMed

Taira, Toshio; Yamaguchi, Sayori; Takahashi, Azusa; Okazaki, Yukako; Yamaguchi, Akihiro; Sakaguchi, Hirohide; Chiji, Hideyuki

2015-11-01

The effects of dietary polyphenols on human health have mainly been discussed in the context of preventing degenerative diseases, particularly cardiovascular diseases and cancer. The antioxidant properties of polyphenols have been widely studied, but it has become clear that the mechanism of action of polyphenols extends beyond the modulation of oxidative stress, as they are poorly absorbed from the digestive tract. The purpose of this study was to clarify the effects of polyphenols on the colonic environment, intestinal barrier function, and gut microbiota. We demonstrated that dietary polyphenols derived from aronia, haskap, and bilberry, markedly elevated the amount of fecal mucin and immunoglobulin A (IgA) as an intestinal barrier function and ameliorated the disturbance in gut microbiota caused by a high fat diet in rats. These results suggest that dietary polyphenols play a significant role in the prevention of degenerative diseases through improvement of the colonic environment without any absorption from the digestive tract.

Dietary polyphenols increase fecal mucin and immunoglobulin A and ameliorate the disturbance in gut microbiota caused by a high fat diet

PubMed Central

Taira, Toshio; Yamaguchi, Sayori; Takahashi, Azusa; Okazaki, Yukako; Yamaguchi, Akihiro; Sakaguchi, Hirohide; Chiji, Hideyuki

2015-01-01

The effects of dietary polyphenols on human health have mainly been discussed in the context of preventing degenerative diseases, particularly cardiovascular diseases and cancer. The antioxidant properties of polyphenols have been widely studied, but it has become clear that the mechanism of action of polyphenols extends beyond the modulation of oxidative stress, as they are poorly absorbed from the digestive tract. The purpose of this study was to clarify the effects of polyphenols on the colonic environment, intestinal barrier function, and gut microbiota. We demonstrated that dietary polyphenols derived from aronia, haskap, and bilberry, markedly elevated the amount of fecal mucin and immunoglobulin A (IgA) as an intestinal barrier function and ameliorated the disturbance in gut microbiota caused by a high fat diet in rats. These results suggest that dietary polyphenols play a significant role in the prevention of degenerative diseases through improvement of the colonic environment without any absorption from the digestive tract. PMID:26566306

Protein-Tannin Interactions of Tryptic Digests of α-Lactalbumin and Procyanidins.

PubMed

Wang, Bei; Heinonen, Marina

2017-01-11

Protein-tannin interactions on a molecular level were investigated by using a model system containing peptides of α-lactalbumin and berry tannins (procyanidins). Oxidation of isolated tryptic peptide LDQWLCEK (m/z 1034) with procyanidin B2 or procyanidin fraction (PF) isolated from aronia juice was monitored by LC-ESI-MS. Procyanidin B2 and PF showed radical scavenging activities toward oxidation of the peptide with the peptide also preventing procyanidin B2 from degradation. Oxidation enhanced the cleavage of peptide between tryptophan and glutamine. Interaction products arising from WLCEK or WLCE residue and degradation product of procyanidin B2 were also identified using both size exclusion chromatography and LC-MS. Tryptophan and lysine were the amino acids most prone to interact with procyanidin B2. The study shows that protein-tannin interaction takes place during oxidation leading to both degradation of the parent compounds and formation of interaction products. This may in turn affect the quality of protein and tannin containing food.

Nymphal survival and habitat distribution of Ixodes scapularis and Amblyomma americanum ticks (Acari:Ixodidae) on Fire Island, New York

USGS Publications Warehouse

Ginsberg, H.S.; Zhioua, E.

1996-01-01

The distribution and survival of Ixodes scapularis and Amblyomma americanum were studied in deciduous and coniferous wooded habitats and in open habitats on Fire Island, New York, USA. The survival of nymphal I. scapularis in field enclosures was greater in forests than in open habitats, suggesting that greater survival contributes to the higher tick population in the woods. The nymphs of each species were more common in deciduous thickets (predominantly Aronia arbutifolia and Vaccinium corynbosum) than in coniferous woods (mostly Pinus rigida) in most but not all years. Larval I. scapularis were more common in coniferous sites in 1994, while the same ticks, as nymphs, were more common in deciduous sites in 1995. The survival of the nymphs was not consistently greater in either the deciduous or coniferous woods. Therefore, factors other than nymphal survival (e.g. larval overwintering survival and tick movement on hosts) probably influenced the relative nymph abundance in different forest types. Overall, the survival of A. americanum was far higher than that of I. scapularis.

Cardioprotective mechanisms of phytochemicals against doxorubicin-induced cardiotoxicity.

PubMed

Abushouk, Abdelrahman Ibrahim; Ismail, Ammar; Salem, Amr Muhammad Abdo; Afifi, Ahmed M; Abdel-Daim, Mohamed M

2017-06-01

Doxorubicin (DOX) is an anthracycline antibiotic, which is effectively used in the treatment of different malignancies, such as leukemias and lymphomas. Its most serious side effect is dose-dependent cardiotoxicity, which occurs through inducing oxidative stress apoptosis. Due to the myelosuppressive effect of dexrazoxane, a commonly-used drug to alleviate DOX-induced cardiotoxicity, researchers investigated the potential of phytochemicals for prophylaxis and treatment of this condition. Phytochemicals are plant chemicals that have protective or disease preventive properties. Preclinical trials have shown antioxidant properties for several plant extracts, such as those of Aerva lanata, Aronia melanocarpa, Astragalus polysaccharide, and Bombyx mori plants. Other plant extracts showed an ability to inhibit apoptosis, such as those of Astragalus polysaccharide, Azadirachta indica, Bombyx mori, and Allium stavium plants. Unlike synthetic agents, phytochemicals do not impair the clinical activity of DOX and they are particularly safe for long-term use. In this review, we summarized the results of preclinical trials that investigated the cardioprotective effects of phytochemicals against DOX-induced cardiotoxicity. Future human trials are required to translate these cardioprotective mechanisms into practical clinical implications. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Rehydration properties of hybrid method dried fruit enriched by natural components

NASA Astrophysics Data System (ADS)

Kowalska, Hanna; Marzec, Agata; Kowalska, Jolanta; Ciurzyńska, Agnieszka; Samborska, Kinga; Bialik, Michał; Lenart, Andrzej

2018-04-01

The aim of the study was to determine the impact of osmotic pre-dehydration and drying of fruit on the rehydration properties of dried fruit. Herein, the effect of fruit juice, applied as a natural enriching substance was very important. In addition, the properties of dried fruits obtained through combined air-drying and subsequent microwave-vacuum drying with `puffing' effect were similar to the freeze-dried fruits, but showed other rehydration properties. As raw material, frozen strawberry (Honeoye variety) and fresh apples (Idared variety) were used in the study. The apples and partially defrosted strawberries were prior dehydrated in solutions of sucrose and a mixture of sucrose with chokeberry juice concentrate at 50°C for 2 h. Next, the fruit samples were dried by one of two ways: air-drying (50°C, 5 h) and microwavevacuum drying for about 360 s; and freeze-drying (30°C, 63 Pa, 24 h). The rehydration was carried out in distilled water (20°C, 5 h). The osmotic pre-dehydration hindered fruit drying process. The impact of drying method became particularly evident while examining the kinetics of rehydration. During the rehydration of the pre-dehydrated dried fruit a slower hydration could be observed. Freeze-dried strawberries absorbed 2-3 times more water than those dried by the `puffing' effect.

Comparison of ice nuclei from fruit juices and their properties

NASA Astrophysics Data System (ADS)

Fiala, Bianca; Felgitsch, Laura; Grothe, Hinrich

2017-04-01

Heterogeneous ice nucleation is a crucial process in the atmosphere. Recent findings indicate the importance of biological ice nuclei (IN) in this process. Pratt et al. (2009) sampled ice-crystal residues at approx. 8 km high altitude over Wyoming, U.S.. They found a third of the dry residues to be biological and further that 60% of the highly abundant mineral dusts to be internally mixed with biological or humic substances. Huffman et al. (2013) showed a burst of biological IN over woodlands connected to rain events. Previous investigations in our group (Pummer et al. 2012) showed that pollen and pollen washing water from several plants native to the boreal forests trigger heterogeneous ice nucleation. Recent work from our group (Felgitsch et al. 2016) showed that several juices of berries are ice nucleation active. Based on this research we examined ice nucleation activity (INA) and general properties of five juices: sea buckthorn, black currant, chokeberry, acerola, and elder berry. We elucidated particle size dependency of the ice nucleation activity to differentiate between coarse ice nucleating particles and nanoparticles or macromolecules. Investigations with different solvents and with chaotropic agents were performed in order to further clarify properties concerning chemical polarity of the IN, and the importance of hydrogen bonds and other structure forming polar interactions. The results are compared to known plant originated IN. Our results suggest similarities in terms of nucleation temperature and structure stability between different IN of plant materials. These properties show a clear differentiation to known bacterial and fungal IN. Felgitsch , L., Bichler, M., Häusler, T., Hitzenberger, R., and Grothe, H.: Heterogeneous freezing of water triggered by berry juices from perenneal plants, submitted, 2016. Huffman, J.A., Prenni, A.J., DeMott, P.J., Pöhlker, C., Mason, R.H., Robinson, N.H., Frohlich-Nowoisky, J., Tobo, Y., Després, V.R., Garcia, E., Gochis, D.J., Harris, E., Müller-Germann, I., Ruzene, C., Schmer, B., Sinha, B., Day, D.A., Andreae, M.O., Jimenez, J.L., Gallagher, M., Kreidenweis, S.M., Bertram, A.K., and Pöschl, U.: High concentrations of biological aerosol particles and ice nuclei during and after rain, Atmos. Chem. Phys., 13, 1651-1664, 2013. Pratt, K.A., DeMott, P.J., French, J.R., Wang, Z., Westphal, D.L., Heymsfield, A.J., Twohy, C.H., Prenni, A.J., and Prather, K.A.: In situ detection of biological particles in cloud ice-crystals, Nat. Geosci., 2, 298-401, 2009. Pummer, B.G., Bauer, H., Bernardi, J., Bleicher, S., and Grothe, H.: Suspendable macromolecules are responsible for ice nucleation activity of birch and conifer pollen, Atmos. Chem. Phys., 12, 2541-2550, 2012.

Changes Caused by Fruit Extracts in the Lipid Phase of Biological and Model Membranes

PubMed Central

Pruchnik, Hanna; Oszmiański, Jan; Sarapuk, Janusz; Kleszczyńska, Halina

2010-01-01

The aim of the study was to determine changes incurred by polyphenolic compounds from selected fruits in the lipid phase of the erythrocyte membrane, in liposomes formed of erythrocyte lipids and phosphatidylcholine liposomes. In particular, the effect of extracts from apple, chokeberry, and strawberry on the red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC liposomes was studied. In the erythrocyte population, the proportions of echinocytes increased due to incorporation of polyphenolic compounds. Fluorimetry with a laurdan probe indicated increased packing density in the hydrophilic phase of the membrane in presence of polyphenolic extracts, the highest effect being observed for the apple extract. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The polyphenolic extracts slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The studies have shown that the phenolic compounds contained in the extracts incorporate into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The compounds also penetrate the outer part of the external lipid layer of liposomes formed of natural and DPPC lipids, changing its packing order. PMID:21423329

Melanoma Inhibition by Anthocyanins Is Associated with the Reduction of Oxidative Stress Biomarkers and Changes in Mitochondrial Membrane Potential.

PubMed

Diaconeasa, Zoriţa; Ayvaz, Huseyin; Ruginǎ, Dumitriţa; Leopold, Loredana; Stǎnilǎ, Andreea; Socaciu, Carmen; Tăbăran, Flaviu; Luput, Lavinia; Mada, Diana Carla; Pintea, Adela; Jefferson, Andrew

2017-12-01

Anthocyanins are water soluble pigments which have been proved to exhibit health benefits. Several studies have investigated their effects on several types of cancer, but little attention has been given to melanoma. The phytochemical content of nine different berry samples was assessed by liquid chromatography followed by electrospray ionization mass spectrometry (LC-ESI + -MS). Twenty-six anthocyanins were identified, after a previous C 18 Sep-pak clean-up procedure. Chokeberry and red grape anthocyanins rich extracts (C-ARE and RG-ARE) were selected to be tested on normal and melanoma cell lines, due to their different chemical pattern. C-ARE composition consists of cyanidin aglycone glycosylated with different sugars; while RG-ARE contains glucosylated derivatives of five different aglycones. Both C-ARE and RG-ARE anthocyanins reduced proliferation, increased oxidative stress biomarkers and diminished mitochondrial membrane potential in melanoma cells, having no negative influence on normal cells. A synergistic response may be attributed to the five different aglycones present in RG-ARE, which proved to exert greater effects on melanoma cells than the mixture of cyanidin derivatives with different sugars (C-ARE). In conclusion, C-ARE and RG-ARE anthocyanins may inhibit melanoma cell proliferation and increase the level of oxidative stress, with opposite effect on normal cells. Therefore, anthocyanins might be recommended as active ingredients for cosmetic and nutraceutical industry. Graphical Abstract ᅟ.

Polyphenolic composition and antioxidant activity of the under-utilised Prunus mahaleb L. fruit.

PubMed

Blando, Federica; Albano, Clara; Liu, Yazheng; Nicoletti, Isabella; Corradini, Danilo; Tommasi, Noemi; Gerardi, Carmela; Mita, Giovanni; Kitts, David D

2016-06-01

The identification of novel plant-based functional foods or nutraceutical ingredients that possess bioactive properties with antioxidant function has recently become important to the food, nutraceutical and cosmetic industries. This study evaluates the polyphenolic composition, identifies bioactive compounds and assays the total antioxidant capacity of Prunus mahaleb L. fruits collected from different populations and sampling years in the countryside around Bari (Apulia Region, Italy). We identified nine polyphenolic compounds including major anthocyanins, coumaric acid derivatives and flavonols from P. mahaleb fruits. The anthocyanin content (in some populations > 5 g kg(-1) fresh weight; FW) in the fruit was comparable to that reported for so-called superfruits such as bilberries, chokeberries and blackcurrants. Coumaric acid derivatives comprised a large portion of the total polyphenolic content in the P. mahaleb fruits. Antioxidant activities, assessed using ORAC and TEAC assays, measured up to 150 and 45 mmol Trolox equivalents kg(-1) FW, respectively. Therefore antioxidant capacity of P. mahaleb fruits is relatively high and comparable to that of superfruit varieties that are often used in commercial nutraceutical products. Our findings suggest that mahaleb fruit (currently not consumed fresh or used in other ways) could serve as a source of bioactive compounds and therefore find interest from the functional food and nutraceutical industries, as a natural food colorant and antioxidant ingredient in the formulation of functional foods. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

The Search for Dietary Supplements to Elevate or Activate Circulating Paraoxonases

PubMed Central

Lou-Bonafonte, José M.; Gabás-Rivera, Clara; Navarro, María A.; Osada, Jesús

2017-01-01

Low levels of paraoxonase 1 (PON1) have been associated with the development of several pathological conditions, whereas high levels have been shown to be anti-atherosclerotic in mouse models. These findings suggest that PON1 could be a good surrogate biomarker. The other members of the family, namely PON2 and PON3, the role of which has been much less studied, deserve more attention. This paper provides a systematic review of current evidence concerning dietary supplements in that regard. Preliminary studies indicate that the response to dietary supplements may have a nutrigenetic aspect that will need to be considered in large population studies or in clinical trials. A wide range of plant preparations have been found to have a positive action, with pomegranate and some of its components being the best characterized and Aronia melanocarpa one of the most active. Flavonoids are found in the composition of all active extracts, with catechins and genistein being the most promising agents for increasing PON1 activity. However, some caveats regarding the dose, length of treatment, bioavailability, and stability of these compounds in formulations still need to be addressed. Once these issues have been resolved, these compounds could be included as nutraceuticals and functional foods capable of increasing PON1 activity, thereby helping with the long-term prevention of atherosclerosis and other chronic ailments. PMID:28212288

The Search for Dietary Supplements to Elevate or Activate Circulating Paraoxonases.

PubMed

Lou-Bonafonte, José M; Gabás-Rivera, Clara; Navarro, María A; Osada, Jesús

2017-02-15

Low levels of paraoxonase 1 (PON1) have been associated with the development of several pathological conditions, whereas high levels have been shown to be anti-atherosclerotic in mouse models. These findings suggest that PON1 could be a good surrogate biomarker. The other members of the family, namely PON2 and PON3, the role of which has been much less studied, deserve more attention. This paper provides a systematic review of current evidence concerning dietary supplements in that regard. Preliminary studies indicate that the response to dietary supplements may have a nutrigenetic aspect that will need to be considered in large population studies or in clinical trials. A wide range of plant preparations have been found to have a positive action, with pomegranate and some of its components being the best characterized and Aronia melanocarpa one of the most active. Flavonoids are found in the composition of all active extracts, with catechins and genistein being the most promising agents for increasing PON1 activity. However, some caveats regarding the dose, length of treatment, bioavailability, and stability of these compounds in formulations still need to be addressed. Once these issues have been resolved, these compounds could be included as nutraceuticals and functional foods capable of increasing PON1 activity, thereby helping with the long-term prevention of atherosclerosis and other chronic ailments.

Hippophae rhamnoides L. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma.

PubMed

Olas, Beata; Kontek, Bogdan; Malinowska, Paulina; Żuchowski, Jerzy; Stochmal, Anna

2016-01-01

Effects of the phenolic fraction from Hippophae rhamnoides fruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation) and the generation of superoxide anion (O2 (-∙)) in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin) were studied in vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2) or H2O2/Fe (a donor of hydroxyl radicals). The tested fraction of H. rhamnoides (0.5- 50 µg/mL; the incubation time: 15 and 60 min) inhibited lipid peroxidation induced by H2O2 or H2O2/Fe. The H. rhamnoides phenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2 or H2O2/Fe. Moreover, the level of O2 (-∙) in platelets significantly decreased. In comparative experiments, the H. rhamnoides fraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL). The obtained results suggest that H. rhamnoides fruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases.

Molecular genetic diversity and maternal origin of Chinese black-bone chicken breeds.

PubMed

Zhu, W Q; Li, H F; Wang, J Y; Shu, J T; Zhu, C H; Song, W T; Song, C; Ji, G G; Liu, H X

2014-04-29

Chinese black-bone chickens are valued for the medicinal properties of their meat in traditional Chinese medicine. We investigated the genetic diversity and systematic evolution of Chinese black-bone chicken breeds. We sequenced the DNA of 520 bp of the mitochondrial cyt b gene of nine Chinese black-bone chicken breeds, including Silky chicken, Jinhu black-bone chicken, Jiangshan black-bone chicken, Yugan black-bone chicken, Wumeng black-bone chicken, Muchuan black-bone chicken, Xingwen black-bone chicken, Dehua black-bone chicken, and Yanjin black-bone chicken. We found 13 haplotypes. Haplotype and nucleotide diversity of the nine black-bone chicken breeds ranged from 0 to 0.78571 and 0.00081 to 0.00399, respectively. Genetic diversity was the richest in Jinhu black-bone chickens and the lowest in Yanjin black-bone chickens. Analysis of phylogenetic trees for all birds constructed based on hyplotypes indicated that the maternal origin of black-bone chickens is predominantly from three subspecies of red jungle fowl. These results provide basic data useful for protection of black-bone chickens and help determine the origin of domestic chickens.

The Power of the Rap: The Black Idiom and the New Black Poetry.

ERIC Educational Resources Information Center

Smitherman, Geneva

Black Arts Literature--of which the New Black Poetry is the most important manifestation--emerged during the past decade as the appropriate artistic counterthrust to Black Power. Rhetoric and shouting aside, this new thrust was, on a very basic level, simply a call to black folks to redefine Blackness and re-evaluate the Black Experience. For the…

For blacks in America, the gap in neighborhood poverty has declined faster than segregation.

PubMed

Firebaugh, Glenn; Acciai, Francesco

2016-11-22

Black residential segregation has been declining in the United States. That accomplishment rings hollow, however, if blacks continue to live in much poorer neighborhoods than other Americans. This study uses census data for all US metropolitan areas in 1980 and 2010 to compare decline in the neighborhood poverty gap between blacks and other Americans with decline in the residential segregation of blacks. We find that both declines resulted primarily from narrowing differences between blacks and whites as opposed to narrowing differences between blacks and Hispanics or blacks and Asians. Because black-white differences in neighborhood poverty declined much faster than black-white segregation, the neighborhood poverty disadvantage of blacks declined faster than black segregation-a noteworthy finding because the narrowing of the racial gap in neighborhood poverty for blacks has gone largely unnoticed. Further analysis reveals that the narrowing of the gap was produced by change in both the medians and shapes of the distribution of poverty across the neighborhoods where blacks, whites, Hispanics, and Asians reside.

IMAGES OF BLACK AMERICANS: Then, "Them," and Now, "Obama!"

PubMed

Fiske, Susan T; Bergsieker, Hilary B; Russell, Ann Marie; Williams, Lyle

2009-01-01

Images of Black Americans are becoming remarkably diverse, enabling Barack Obama to defy simple-minded stereotypes and succeed. Understood through the Stereotype Content Model's demonstrably fundamental trait dimensions of perceived warmth and competence, images of Black Americans show three relevant patterns. Stereotyping by omission allows non-Blacks to accentuate the positive, excluding any lingering negativity but implying it by its absence; specifically, describing Black Americans as gregarious and passionate suggests warmth but ignores competence and implies its lack. Obama's credentials prevented him from being cast as incompetent, though the experience debate continued. His legendary calm and passionate charisma saved him on the warmth dimension. Social class subtypes for Black Americans differentiate dramatically between low-income Blacks and Black professionals, among both non-Black and Black samples. Obama clearly fit the moderately warm, highly competent Black-professional subtype. Finally, the campaign's events (and nonevents) allowed voter habituation to overcome non-Blacks' automatic emotional vigilance to Black Americans.

Black English and Black Attitudes

ERIC Educational Resources Information Center

Shores, David L.

1974-01-01

Examines attitudes in the Black community towards the topic of Black English and specifically the controversy about the relationship of the speech of Blacks to that of Whites, the distinctive features in the speaking and writing of Black college students, and the attitudes of Black educators. Available from South Atlantic Modern Language…

77 FR 50668 - Proposed Flood Elevation Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-22

... County, Texas, and Incorporated Areas. Specifically, it addresses the flooding sources: Black Fork Creek, Black Fork Creek Tributary BF-1, Black Fork Creek Tributary BF-M-1, Black Fork Creek Tributary D, Black Fork Creek Tributary D-1, Black Fork Creek Tributary D-2, Black Fork Creek Tributary D-3, Butler Creek...

The Black Man's Burden: The White Clinician

ERIC Educational Resources Information Center

Wesson, K. Alan

1975-01-01

The stated purpose of this article is to advocate that those dealing with black people and black problems have some appreciable degree of knowledge concerning black culture, black jargon, black behavior, and the black or Afro-American psyche: only then can therapy be seen as satisfying the purposes for which it was initially intended. (Author/JM)

The Crisis in Black and Black.

ERIC Educational Resources Information Center

Hutchinson, Earl Ofari

These essays explore why the historic conflict between blacks and whites in the United States has become a crisis that divides many African Americans. The changing racial dynamic is not marked by conflicts. between the black middle class and the poor, black men and women, the black intellectual elite and rappers, black politicians and the urban…

Can Blacks Be Racists? Black-on-Black Principal Abuse in an Urban School Setting

ERIC Educational Resources Information Center

Khalifa, Muhammad

2015-01-01

This study examines Black student and parental perceptions of exclusionary practices of Black school principals. I ask why students and parents viewed two Black principals as contributing to abusive and exclusionary school environments that marginalized Black students. After a two-year ethnographic study, it was revealed that exclusionary…

In the Absence of Ideology: Blacks in Colonial America and the Modern Black Experience.

ERIC Educational Resources Information Center

Jones, Rhett S.

1988-01-01

The experiences of Whites and Blacks in the American Colonial period were strikingly different. Blacks were committed to Whites' Enlightenment ideology, which did not reflect Black experience. Blacks in the Colonial era were unable to create political structures capable of making sense of the Black experience. (BJV)

IMAGES OF BLACK AMERICANS

PubMed Central

Fiske, Susan T.; Bergsieker, Hilary B.; Russell, Ann Marie; Williams, Lyle

2013-01-01

Images of Black Americans are becoming remarkably diverse, enabling Barack Obama to defy simple-minded stereotypes and succeed. Understood through the Stereotype Content Model’s demonstrably fundamental trait dimensions of perceived warmth and competence, images of Black Americans show three relevant patterns. Stereotyping by omission allows non-Blacks to accentuate the positive, excluding any lingering negativity but implying it by its absence; specifically, describing Black Americans as gregarious and passionate suggests warmth but ignores competence and implies its lack. Obama’s credentials prevented him from being cast as incompetent, though the experience debate continued. His legendary calm and passionate charisma saved him on the warmth dimension. Social class subtypes for Black Americans differentiate dramatically between low-income Blacks and Black professionals, among both non-Black and Black samples. Obama clearly fit the moderately warm, highly competent Black-professional subtype. Finally, the campaign’s events (and nonevents) allowed voter habituation to overcome non-Blacks’ automatic emotional vigilance to Black Americans. PMID:24235974

Quinine sensitivity influences the acceptance of sea-buckthorn and grapefruit juices in 9- to 11-year-old children.

PubMed

Hartvig, Ditte; Hausner, Helene; Wendin, Karin; Bredie, Wender L P

2014-03-01

The acceptance of novel foods by children is related to a number of factors, and differences in taste sensitivity may form some specific challenges. High sensitivity might be a barrier to the acceptance of sour/bitter products by children. This study investigated the effect of sensitivity to bitter, sour, sweet, and salty tastes on the acceptance of Nordic juices in 9- to 11-year-old children. A total of 328 children were subjected to two taste sensitivity tests for quinine, citric acid, sucrose, and NaCl. Their acceptance of six juices (carrot, rosehip, sea-buckthorn, lingonberry, grapefruit, and aronia) was measured. Bitter sensitivity was found to be significantly correlated to the intake of the sweet sea-buckthorn and lingonberry juices; the most bitter-sensitive children exhibited the highest intake of these juices. The opposite relationship was found for bitter sensitivity and the intake of the bitter grapefruit juice. Sour, sweet, and salt sensitivities did not affect the intake of any of the juices. Liking scores were not affected by sensitivity. In conclusion, bitter sensitivity appears to influence food intake in children to a greater extent than sour, sweet, or salt sensitivity. Bitter-sensitive children exhibited a reduced intake of grapefruit juice and a higher intake of sucrose-sweetened juices. Thus, bitter sensitivity might be a challenge in the acceptance of certain bitter foods. Copyright © 2013 Elsevier Ltd. All rights reserved.

Hippophae rhamnoides L. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma

PubMed Central

Olas, Beata; Kontek, Bogdan; Malinowska, Paulina; Żuchowski, Jerzy; Stochmal, Anna

2016-01-01

Effects of the phenolic fraction from Hippophae rhamnoides fruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation) and the generation of superoxide anion (O2 −∙) in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin) were studied in vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2) or H2O2/Fe (a donor of hydroxyl radicals). The tested fraction of H. rhamnoides (0.5– 50 µg/mL; the incubation time: 15 and 60 min) inhibited lipid peroxidation induced by H2O2 or H2O2/Fe. The H. rhamnoides phenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2 or H2O2/Fe. Moreover, the level of O2 −∙ in platelets significantly decreased. In comparative experiments, the H. rhamnoides fraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL). The obtained results suggest that H. rhamnoides fruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases. PMID:26933473

"Be Real Black for Me": Imagining BlackCrit in Education

ERIC Educational Resources Information Center

Dumas, Michael J.; ross, kihana miraya

2016-01-01

The authors put forward a theorization of a Black Critical Theory, or what might be called BlackCrit, within, and in response to, Critical Race Theory, and then outline ways that BlackCrit in education helps us to more incisively analyze how the specificity of (anti)blackness matters in explaining how Black bodies become marginalized, disregarded,…

Low-mass black holes as the remnants of primordial black hole formation.

PubMed

Greene, Jenny E

2012-01-01

Bridging the gap between the approximately ten solar mass 'stellar mass' black holes and the 'supermassive' black holes of millions to billions of solar masses are the elusive 'intermediate-mass' black holes. Their discovery is key to understanding whether supermassive black holes can grow from stellar-mass black holes or whether a more exotic process accelerated their growth soon after the Big Bang. Currently, tentative evidence suggests that the progenitors of supermassive black holes were formed as ∼10(4)-10(5) M(⊙) black holes via the direct collapse of gas. Ongoing searches for intermediate-mass black holes at galaxy centres will help shed light on this formation mechanism.

Survey of coatings for solar collectors. [ceramic enamels and chromium

NASA Technical Reports Server (NTRS)

Mcdonald, G. E.

1974-01-01

Ceramic enamel is found to be more solar selective, (i.e., has high solar absorptance in combination with low infrared emittance) than organic enamel, but neither is as solar selective as black chrome, black copper, black zinc, or black nickel. Ceramic enamel is matched only by black chrome in durability and wide availability. Ceramic enamel and organic enamel have approximately the same cost, and both are currently slightly lower in cost than black chrome, black copper, or black zinc. Black nickel is relatively unavailable and, because of that, realistic cost comparisons are not possible.

Validity of black hole complementarity in the BTZ black hole

NASA Astrophysics Data System (ADS)

Gim, Yongwan; Kim, Wontae

2018-01-01

Based on the gedanken experiment for black hole complementarity in the Schwarzschild black hole, we calculate the energy required to duplicate information in the BTZ black hole under the assumption of absorbing boundary condition and its dual solution of the black string, respectively, in order to justify the validity of the no-cloning theorem in quantum mechanics. For the BTZ black hole, the required energy for the duplication of information can be made fairly small, whereas for the black string it exceeds the total mass of the black string, although they are related to each other under the dual transformation. So, the duplication of information might be possible in the BTZ black hole in contrast to the case of the black string, so that the no-cloning theorem could be violated for the former case. To save the duplication of information for the BTZ black hole, we perform an improved gedanken experiment by using the local thermodynamic quantities near the horizon rather than those defined at infinity, and show that the no-cloning theorem could be made valid even in the BTZ black hole. We also discuss how this local treatment for the no-cloning theorem can be applied to the black string as well as the Schwarzschild black hole innocuously.

Understanding the New Black Poetry: Black Speech and Black Music as Poetic References.

ERIC Educational Resources Information Center

Henderson, Stephen

Oral tradition, both rural and urban, forms an infrastructure for this anthology, which presents selections of black poetry with an emphasis on the poetry of the sixties. Based on the thesis that the new black poetry's main referents are black speech and black music, the anthology includes examples from the oral tradition of folk sermon,…

Black and white girls' racial preferences in media and peer choices and the role of socialization for black girls.

PubMed

O'Connor, L A; Brooks-Gunn, J; Graber, J

2000-09-01

This exploratory research compared Black and White girls' racial preferences as exhibited through their media (music and television) and peer choices. The sample included 140 8- and 9-year-old Black and White girls of various socioeconomic levels. Findings suggested that both Black and White girls have more Black music preferences than White or no-race music preferences. Also, both Black and White girls made more White television program choices than Black or no-race choices. In their peer selections, all girls preferred same-race peers. Black mothers who engaged in racial socialization practices had girls who were more likely to prefer Black music and television to the other categories. Further, Black mothers who promoted more cultural distance and mothers who were poor had girls with more same-race peer preferences.

News and Views.

ERIC Educational Resources Information Center

Journal of Blacks in Higher Education, 2002

2002-01-01

Presents a collection of articles on blacks in higher education, including such topics as: older black students; blacks in academic nursing; black medical school enrollment; blacks in academic psychology; black colleges being ignored by the Rhodes Scholarship Committee; school desegregation in Cambridge, Massachusetts; Louis Farrakhan as a GOP…

News and Views.

ERIC Educational Resources Information Center

Journal of Blacks in Higher Education, 2002

2002-01-01

This collection of articles focuses on such topics as the importance of college education among black business leaders; ranking of black colleges; black student recruitment; black scholars and African American publishing; Blacks in the life sciences; segregation in college sports; black enrollments; affirmative action; and differences in student…

Spectral reflectance properties of black chrome for use as a solar selective coating

NASA Technical Reports Server (NTRS)

Mcdonald, G. E.

1974-01-01

The NASA-Lewis Research Center has determined that a widely available commercially electroplated decorative finish known as black chrome has desirable solar selective properties. Black chrome electroplated coating has high absorbtance in the solar spectrum and low emissivity in the 250 F blackbody thermal spectrum. The spectral reflectance properties of a commercially prepared black chrome on steel have been measured. Values are presented for reflectance of the black chrome, and compared with the reflectance of black paint and with two available samples of black nickel which had been prepared for solar selective properties. The reflectance of black chrome, of the two black nickels, and of black paint integrated over the solar spectrum for air mass 2 were 0.132, 0.123, 0.133, and 0.033, respectively. The reflectance of the black chrome, two black nickels, and of the black paint integrated over the blackbody spectrum for 250 F from 3 to 15 microns are 0.912, 0.934, 0.891, and 0.033, respectively. These reflectance measurements indicate absorptivity-to-emissivity values of 9.8, 13.8, 8.0, and 1.00, respectively.

Contextual Predictors of Injection Drug Use Among Black Adolescents and Adults in US Metropolitan Areas, 1993–2007

PubMed Central

West, Brooke; Linton, Sabriya; Hunter-Jones, Josalin; Zlotorzynska, Maria; Stall, Ron; Wolfe, Mary E.; Williams, Leslie; Hall, H. Irene; Cleland, Charles; Tempalski, Barbara; Friedman, Samuel R.

2016-01-01

Objectives. We sought to determine whether contextual factors shape injection drug use among Black adolescents and adults. Methods. For this longitudinal study of 95 US metropolitan statistical areas (MSAs), we drew annual MSA-specific estimates of the prevalence of injection drug use (IDU) among Black adolescents and adults in 1993 through 2007 from 3 surveillance databases. We used existing administrative data to measure MSA-level socioeconomic status; criminal justice activities; expenditures on social welfare, health, and policing; and histories of Black uprisings (1960–1969) and urban renewal funding (1949–1974). We regressed Black IDU prevalence on these predictors by using hierarchical linear models. Results. Black IDU prevalence was lower in MSAs with declining Black high-school dropout rates, a history of Black uprisings, higher percentages of Black residents, and, in MSAs where 1992 White income was high, higher 1992 Black income. Incarceration rates were unrelated. Conclusions. Contextual factors shape patterns of drug use among Black individuals. Structural interventions, especially those that improve Black socioeconomic security and political strength, may help reduce IDU among Black adolescents and adults. PMID:26691126

Differences Between Black Youth Who Support the Black Panthers and the NAACP

ERIC Educational Resources Information Center

Levine, Daniel U.; And Others

1973-01-01

This study compares the attitudes of black youth who have a favorable orientation toward the Black Panthers with those of black youth who appear to be oriented toward the NAACP, using data collected in five black segregated high schools. (Author/JM)

Spectral reflectance properties of black chrome for use as a solar selective coating

NASA Technical Reports Server (NTRS)

Mcdonald, G. E.

1974-01-01

The NASA-Lewis Research Center has determined that a widely available commercially electroplated decorative finish known as black chrome has desirable solar selective properties. The spectral reflectance properties of a commercially prepared black chrome on steel were measured. Values are presented for reflectance of the black chrome, and compared with the reflectance of black paint (Nextel) and with two available samples of black nickel which had been prepared for solar selective properties. The reflectance of black chrome, of the two black nickels, and of black paint integrated over the solar spectrum for air mass 2 were 0.132, 0.123, 0.133, and 0.033, respectively. The reflectance of the black chrome, two black nickels, and of the black paint integrated over the blackbody spectrum for 250 F from 3 to 15 microns are 0.912, 0.934, 0.891, and 0.033, respectively. These reflectance measurements indicate absorptivity-to-emissivity values of 9.8, 13.8, 8.0, and 1.00, respectively.

Staying in the Hood: Black Lesbian and Transgender Women and Identity Management in North Philadelphia.

PubMed

Brooks, Siobhan

2016-12-01

The concept Don't Ask, Don't Tell regarding Black LGBT sexuality in Black communities has been an acceptable form of identity management for Black LGBT people. In other words, Black LGBT people are accepted as long as they are not vocal about their sexuality. However, this is changing with the issue of gay marriage, which is creating a space where Black LGBT people are more open about their gender identity and sexuality in heterosexual Black spaces. This new form of openness allows Black LGBT people to "stay in" their communities, as opposed to coming out. In this article I examine how Black LGBT women in North Philadelphia stay in their communities: being politically active regarding LGBT issues, disengaging from LGBT issues, passing, and educating straight Black people about issues affecting the Black LGBT community. I conclude with implications of staying in and intersectionality among Black heterosexual and LGBT women fighting for social change.

For blacks in America, the gap in neighborhood poverty has declined faster than segregation

PubMed Central

Acciai, Francesco

2016-01-01

Black residential segregation has been declining in the United States. That accomplishment rings hollow, however, if blacks continue to live in much poorer neighborhoods than other Americans. This study uses census data for all US metropolitan areas in 1980 and 2010 to compare decline in the neighborhood poverty gap between blacks and other Americans with decline in the residential segregation of blacks. We find that both declines resulted primarily from narrowing differences between blacks and whites as opposed to narrowing differences between blacks and Hispanics or blacks and Asians. Because black–white differences in neighborhood poverty declined much faster than black–white segregation, the neighborhood poverty disadvantage of blacks declined faster than black segregation—a noteworthy finding because the narrowing of the racial gap in neighborhood poverty for blacks has gone largely unnoticed. Further analysis reveals that the narrowing of the gap was produced by change in both the medians and shapes of the distribution of poverty across the neighborhoods where blacks, whites, Hispanics, and Asians reside. PMID:27821759

Heating affects the content and distribution profile of isoflavones in steamed black soybeans and black soybean koji.

PubMed

Huang, Ru-Yue; Chou, Cheng-Chun

2008-09-24

Steamed black soybeans and black soybean koji, a potentially functional food additive, were subjected to heating at 40-100 degrees C for 30 min. It was found that steamed black soybeans and black soybean koji after heating at 80 degrees C or higher generally showed reduced contents of malonylglucoside, acetylglucoside, and aglycone isoflavone and an increased content of beta-glucoside. A lower reduction in malonylglucoside and acetylglucoside isoflavone but greater reduction in aglycone content was noted in steamed black soybeans compared to black soybean koji after a similar heat treatment. After 30 min of heating at 100 degrees C, steamed black soybean retained ca. 90.3 and 83.8%, respectively, of its original malonylglucoside and acetylglucoside isoflavone, compared to lower residuals of 80.9 and 78.8%, respectively, for black soybean koji. In contrast, the heated black soybeans showed an aglycone residual of 68.0%, which is less than the 80.0% noted with the heated black soybean koji.

To Find an Image: Black Films from Uncle Tom to Super Fly.

ERIC Educational Resources Information Center

Murray, James P.

This book surveys the roles of blacks in the film industry. The introduction discusses the question if why there should be a black cinema. The seventeen sections of the book discuss such people and topics as the black actor, movies and life and black people, Sidney Poitier as the black superstar, Diahann Carroll and black female singers in movies,…

Black Women in the Labor Force. Facts on Working Women No. 90-4.

ERIC Educational Resources Information Center

Women's Bureau (DOL), Washington, DC.

During the 1980s, the population of black women aged 16 years and older in the United States increased by 17.2%, and labor force participation for black women increased by 29%. In 1987, black women accounted for 50% of total black employment. The unemployment rate for black teenagers in 1990 was 30% (versus 10.8% for all black women). Labor force…

From navigation to negotiation: an examination of the lived experiences of Black gay male alumni of historically Black colleges and universities.

PubMed

Ford, Obie

2015-01-01

This research presents an examination of Black gay men and their lived experiences while undergraduates at historically Black colleges and universities (HBCUs). Based on 10 in-depth interviews with self-identified Black gay men, the author presents four emergent themes, which reveal the complex ways in which Black gay men navigate and negotiate the intersections of their multiple identities as related to race, sexual orientation, and gender at HBCUs. The findings of this research have implications for larger discussions of community, Black masculinity, and gay identity in predominantly Black and non-Black contexts.

Framing black boys: parent, teacher, and student narratives of the academic lives of black boys.

PubMed

Rowley, Stephanie J; Ross, Latisha; Lozada, Fantasy T; Williams, Amber; Gale, Adrian; Kurtz-Costes, Beth

2014-01-01

The discourse on Black boys tends to suggest that Black boys are in complete peril. We begin with evidence that Black boys are excelling in certain contexts (i.e., in certain states, in certain schools, and in certain courses). We then discuss the ways in which the narratives used by parents, teachers, and Black boys themselves may serve to further reinforce views that Black boys are beyond hope. Research on Black parents suggests that they tend to view their sons as vulnerable and have lower expectations for sons than for daughters. Studies of teachers show that they tend to view Black boys as unteachable, as social problems, and as scary. Research on Black boys shows that they are sometimes complicit in supporting these narratives by engaging in negative or ste reotypical behavior. We also include recent research that includes counter-narratives of Black boys. We end with suggestions for future research.

BLACK PRETERM BIRTH RISK IN NON-BLACK NEIGHBORHOODS: EFFECTS OF HISPANIC, ASIAN, AND NON-HISPANIC WHITE ETHNIC DENSITIES

PubMed Central

Mason, Susan M.; Kaufman, Jay S.; Daniels, Julie L.; Emch, Michael E.; Hogan, Vijaya K.; Savitz, David A.

2013-01-01

Purpose Studies of ethnic density and health in the United States have documented poorer health outcomes in black compared to non-black neighborhoods, but few studies have considered the identities of the non-black populations. Methods New York City birth records from 1995 through 2003 and a spatial measure of ethnic density were used to examine preterm birth risks among non-Hispanic black women associated with non-Hispanic white, Hispanic, Asian, and non-Hispanic black neighborhood densities. Logistic regression models were used to estimate the effect on black preterm birth risks of replacing white neighbors with Hispanic, Asian, and black neighbors. Risk differences were computed for changes from the 10th to the 90th percentiles of ethnic density. Results Increasing Hispanic density was associated with reduced preterm birth risks among non-Hispanic black women, especially if the black women were foreign-born (RD=−19.1 per 1,000 births; 95% CI: −28.6, −9.5). Estimates for increasing Asian density were null. Increasing black density was associated with increasing black preterm birth risk, with a threshold at higher levels of black density. Conclusions The low risks of preterm birth among foreign-born non-Hispanic black women in majority-Hispanic neighborhoods may be related to protective psychosocial or nutritional factors in Hispanic neighborhoods. PMID:21737050

Smoking Cessation among Blacks.

ERIC Educational Resources Information Center

Stotts, R. Craig; And Others

1991-01-01

Lung cancer is a serious health problem among blacks, with a mortality rate of 119 per 100,000 black males, compared to 81 per 100,000 for white males. Smoking cessation efforts are most successful when tailored to the black community, using black community networks and broadcast media for black audiences. (SLD)

What Black Educators are Saying.

ERIC Educational Resources Information Center

Wright, Nathan, Jr., Ed.

Contents of this book are comprised of five groups of articles: Part I. The Black Educator: "Education for black humanism; a way of approaching it," Preston Wilcox; "The new black dimension in our society," Olivia Pearl Stokes; "The black teacher and black Power," Leslie Campbell; and, "The difference," Leslie Campbell. Part II. The White…

The Meaning of Black Music

ERIC Educational Resources Information Center

Byrd, Donald

1976-01-01

The author states that black music is an expression of the black experience. Counseling engages the experience of the person(s) counseled but ignores an important expressive outlet blacks have used in their struggle for survival. If counselors are to understand black people, they must listen to and understand black music. (Author)

Poverty among Young Children in Black Immigrant, US-Born Black, and Non-Black Immigrant Families: The Role of Familial Contexts. Discussion Paper Series. DP 2010-02

ERIC Educational Resources Information Center

Thomas, Kevin J. A.

2010-01-01

This study examines how familial contexts affect poverty disparities between the children of immigrant and US-born Blacks, and among Black and non-Black children of immigrants. Despite lower gross child poverty rates in immigrant than US-born Black families, accounting for differences in family structure reveals that child poverty risks among…

REVIEWS OF TOPICAL PROBLEMS: Search for black holes

NASA Astrophysics Data System (ADS)

Cherepashchuk, Anatolii M.

2003-04-01

Methods and results of searching for stellar mass black holes in binary systems and for supermassive black holes in galactic nuclei of different types are described. As of now (June 2002), a total of 100 black hole candidates are known. All the necessary conditions Einstein's General Relativity imposes on the observational properties of black holes are satisfied for candidate objects available, thus further assuring the existence of black holes in the Universe. Prospects for obtaining sufficient criteria for reliably distinguishing candidate black holes from real black holes are discussed.

Refinement in black chrome for use as a solar selective coating

NASA Technical Reports Server (NTRS)

Mcdonald, G. E.

1974-01-01

Black chrome is significant as a solar selective coating because the current extensive use of black chrome in the electroplating industry as a durable decorative finish makes black chrome widely available on a commercial scale and potentially low in cost as a solar selective coating. Black-chrome deposits were modified by underplating with dull nickel or by being plated on rough surfaces. Both of these procedures increased the visible absorptance. There was no change in the infrared reflectance for the dull-nickel - black-chrome combination from that reported for the bright-nickel - black-chrome combination. However, the bright-nickel - black-chrome coating plated on rough surfaces indicated a slight decrease in infrared reflectance. As integrated over the solar spectrum for air mass 2, the reflectance of the dull-nickel - black-chrome coating was 0.077, of the bright-nickel - black-chrome coating plated on a 0.75-micron (30-microinch) surface was 0.070, of the bright-nickel - black-chrome coating plated on a 2.5 micron (100-microinch) surface was 0.064. The corresponding values for the bright-nickel - black-chrome coating on a 0.0125-micron (0.5-microinch) surface, two samples of black nickel, and two samples of Nextrel black paint were 0.132, 0.123, 0.133, and 0.033, respectively.

Health disparity in black women: lack of pharmaceutical advertising in black vs. white-oriented magazines.

PubMed Central

Omonuwa, S. C.

2001-01-01

CONTEXT: Racial disparities in health care between black women and white women may be attributed in part to socioeconomic status and lack of insurance, but also may be due to lack of the dissemination of health information in black communities via black popular magazines. OBJECTIVE: Comparison of the number and type of pharmaceutical advertisements between black-oriented magazines and white-oriented magazines. DESIGN: Descriptive study. SETTING: Morehouse School of Medicine. MAIN OUTCOME MEASURES: Recording of the type and number of over-the-counter and prescription drug advertisements. RESULTS: Five black-oriented magazines (Black Woman, Black Elegance, Essence, Ebony, and Upscale) and 5 white-oriented magazines (Family Circle, Working Mother, Good Housekeeping, Ladies' Home Journal, Women's Day) were evaluated for 3 months from June-August, 2000. White-oriented magazines had four to eight times more pharmaceutical ads than black-oriented magazines. Types of medications advertised in the white-oriented magazines and not the black-oriented magazines were, for example, cholinesterase inhibitors, calcium supplements, COX II-inhibitors, intranasal steroids, anorexiants, proton pump inhibitors, and smoking deterrent agents. Conversely, medications advertised in the black-oriented magazines and not the white-oriented magazines were antiviral agents and oral contraceptives. Pharmaceutical companies gave several reasons for the disparity, including the explanation that their particular company was advertising about HIV in the black community. CONCLUSIONS: A barrier to equitable health care for black women may be a low prioritization for health prevention and health management. This low prioritization or disinterest may be a reflection of the black magazine that she is reading due to the lack of pharmaceutical advertisements in black-oriented magazines. The result of this disinterest of black females may be seen in the increased morbidity and mortality for selected diseases. PMID:11491276

Black Hole Universe Model for Explaining GRBs, X-Ray Flares, and Quasars as Emissions of Dynamic Star-like, Massive, and Supermassive Black Holes

NASA Astrophysics Data System (ADS)

Zhang, Tianxi

2014-01-01

Slightly modifying the standard big bang theory, the author has recently developed a new cosmological model called black hole universe, which is consistent with Mach’s principle, governed by Einstein’s general theory of relativity, and able to explain all observations of the universe. Previous studies accounted for the origin, structure, evolution, expansion, cosmic microwave background radiation, and acceleration of the black hole universe, which grew from a star-like black hole with several solar masses through a supermassive black hole with billions of solar masses to the present state with hundred billion-trillions of solar masses by accreting ambient matter and merging with other black holes. This study investigates the emissions of dynamic black holes according to the black hole universe model and provides a self-consistent explanation for the observations of gamma ray bursts (GRBs), X-ray flares, and quasars as emissions of dynamic star-like, massive, and supermassive black holes. It is shown that a black hole, when it accretes its ambient matter or merges with other black holes, becomes dynamic. Since the event horizon of a dynamic black hole is broken, the inside hot (or high-frequency) blackbody radiation leaks out. The leakage of the inside hot blackbody radiation leads to a GRB if it is a star-like black hole, an X-ray flare if it is a massive black hole like the one at the center of the Milky Way, or a quasar if it is a supermassive black hole like an active galactic nucleus (AGN). The energy spectra and amount of emissions produced by the dynamic star-like, massive, and supermassive black holes can be consistent with the measurements of GRBs, X-ray flares, and quasars.

Health disparity in black women: lack of pharmaceutical advertising in black vs. white-oriented magazines.

PubMed

Omonuwa, S C

2001-01-01

Racial disparities in health care between black women and white women may be attributed in part to socioeconomic status and lack of insurance, but also may be due to lack of the dissemination of health information in black communities via black popular magazines. Comparison of the number and type of pharmaceutical advertisements between black-oriented magazines and white-oriented magazines. Descriptive study. Morehouse School of Medicine. Recording of the type and number of over-the-counter and prescription drug advertisements. Five black-oriented magazines (Black Woman, Black Elegance, Essence, Ebony, and Upscale) and 5 white-oriented magazines (Family Circle, Working Mother, Good Housekeeping, Ladies' Home Journal, Women's Day) were evaluated for 3 months from June-August, 2000. White-oriented magazines had four to eight times more pharmaceutical ads than black-oriented magazines. Types of medications advertised in the white-oriented magazines and not the black-oriented magazines were, for example, cholinesterase inhibitors, calcium supplements, COX II-inhibitors, intranasal steroids, anorexiants, proton pump inhibitors, and smoking deterrent agents. Conversely, medications advertised in the black-oriented magazines and not the white-oriented magazines were antiviral agents and oral contraceptives. Pharmaceutical companies gave several reasons for the disparity, including the explanation that their particular company was advertising about HIV in the black community. A barrier to equitable health care for black women may be a low prioritization for health prevention and health management. This low prioritization or disinterest may be a reflection of the black magazine that she is reading due to the lack of pharmaceutical advertisements in black-oriented magazines. The result of this disinterest of black females may be seen in the increased morbidity and mortality for selected diseases.

Fear of a Black femme: The existential conundrum of embodying a Black femme identity while being a professor of Black, queer, and feminist studies.

PubMed

Story, Kaila Adia

2017-10-02

Although a Black femme identity has been defined and embodied by many as an identity with Black feminist roots and revolutionary potentials, Black femmes are still rendered hypervisible and invisible through racist and heteronormative politics. Similarly, embodying a Black femme identity as a professor in academia often engenders these same pretenses of hypervisibility and invisibility. This essay explores what this existential conundrum has been for me as both a Black femme and professor of Black queer and feminist studies, while illuminating the mix of forces within academia that have attempted to stifle my chosen sexual identity and gendered performance.

Are LIGO's Black Holes Made from Smaller Black Holes?

NASA Astrophysics Data System (ADS)

Fishbach, Maya; Holz, Daniel; Farr, Ben; LIGO Collaboration

2017-01-01

We consider the hierarchical merger model for the formation of stellar mass black holes (such as the binary black holes observable by LIGO). In the hierarchical merger model, each black hole in a black hole binary is the result of a merger of two lesser black holes from a previous generation, and the previous generation's black holes may themselves be merger products of an even earlier generation. We apply the formulas of Hofmann, Barausse and Rezzolla (2016) to show that if black holes form in this hierarchical merger scenario, their spin magnitudes follow a certain probability distribution. We demonstrate how to compare this spin distribution to LIGO spin measurements in order to constrain the hierarchical merger scenario.

The Black Dentist Workforce in the United States

PubMed Central

Mertz, Elizabeth; Calvo, Jean; Wides, Cynthia; Gates, Paul

2017-01-01

Objectives The purpose of this paper is to describe the Black dentist workforce, the practice patterns of providers, and their contributions to oral health care for minority and underserved patients. Methods A national sample survey of underrepresented minority dentists was conducted in 2012 and received a 32.6% response rate for self-reported Black dentists. Data were weighted for selection and response bias to be nationally representative. Descriptive and multivariable statistics were computed to provide a workforce profile of Black dentists. National comparisons are provided from published data. Results Among all Black dentists (weighted n=6,254), 76.6% self-identify as African-American, 13.2% as African, and 10.3% as Afro-Caribbean. The largest share of Black dentists are male, married, heterosexual, born in the U.S. and raised in a medium to large city. One third of Black dentists were the first in their family to graduate from college. Black dentists report higher average educational debt than all dental students, graduates from International Dentist Programs having the greatest debt. Traditional practices (i.e., private practices) dominate, with 67.1% of Black dentists starting out in this setting and 73.5% currently in the setting. Black dentists care for a disproportionate share of Black patients, with an average patient mix that is 44.9% Black. Two in five Black dentists reported their patient pool is made up of more than 50% Black patients. Conclusions The underrepresentation for Black dentists is extraordinary, and the Black dentists that are in practice are shouldering a disproportionate share of dental care for minority and underserved communities. PMID:27966789

On the Charter Question: Black Marxism and Black Nationalism

ERIC Educational Resources Information Center

Stern, Mark; Hussain, Khuram

2015-01-01

This article brings two black intellectual traditions to bear on the question of charter schools: black Marxism and black nationalism. The authors examine the theoretical and rhetorical devices used to talk about charters schools by focusing on how notions of "black liberation" are deployed by the charter movement, and to what end. The…

Black Newspapers: Overlooked Barometers. NCRIEEO Tipsheet, Number 1.

ERIC Educational Resources Information Center

Columbia Univ., New York, NY. National Center for Research and Information on Equal Educational Opportunity.

Black newspapers should not go unnoticed by school administrators, as they too often are. Black people have a long and proud history of intraracial communication. More than 200 "black" periodicals work hard at forming, leading, and interpreting for black communities. No black community is totally dependent upon the standard white-owned and…

The Impatient Press: Placing Black Newspapers in the Ideologies of Black Progress.

ERIC Educational Resources Information Center

Caspari, Genevieve G.

Unlike its white counterpart, the black press has historically exceeded its role of information source. Specialized black media were founded to decry racial conflict and gain civil rights. Only rarely, and secondarily, have black papers achieved commercial success. The purposes of black papers have changed with the society they serve. Ideologies…

Black Boundary Lines: Race, Class and Gender among Black Undergraduate Students

ERIC Educational Resources Information Center

Morales, Erica Marie

2012-01-01

Intra-group differences among Black undergraduate students remain understudied. To gain a more nuanced understanding of Black student life, we must examine how other social locations, like gender and class, connect to the racialized experiences of Black students. This dissertation argues that for Black students, class and gender, along with race,…

"Black Like Me": Reframing Blackness for Decolonial Politics

ERIC Educational Resources Information Center

Dei, George J. Sefa

2018-01-01

From a particular vantage point, as an African-born scholar with a politics to affirm my Black subjectivity and Indigeneity in a diasporic context, my article engages a (re)theorization of Blackness for decolonial politics. Building on existing works of how Black scholars, themselves, have theorized Blackness, and recognizing the fluid,…

The Black Lives Matter Movement and Historically Black Colleges and Universities

ERIC Educational Resources Information Center

Gasman, Marybeth

2017-01-01

This article looks at the Black Lives Matter Movement and Historically Black Colleges and Universities. Historically Black Colleges and Universities (HBCUs) continue to play an important role in society. However, what the Black Lives Matter movement shows consistently is that predominantly White institutions need to change, to step up and embrace…

Ten Myths, Half-Truths and Misunderstandings About Black History.

ERIC Educational Resources Information Center

Ruffins, Paul

1997-01-01

Common myths and misconceptions about Blacks in American history and evidence that refutes them are presented. Issues addressed include Black enslavement patterns, social status within the Black community based on skin color, the legality of slaves learning to read, resistance to slavery, African influences in modern Black culture, Black names and…

Black string in dRGT massive gravity

NASA Astrophysics Data System (ADS)

Tannukij, Lunchakorn; Wongjun, Pitayuth; Ghosh, Suchant G.

2017-12-01

We present a cylindrically symmetric solution, both charged and uncharged, which is known as a black string solution to the nonlinear ghost-free massive gravity found by de Rham, Gabadadze, and Tolley (dRGT). This "dRGT black string" can be thought of as a generalization of the black string solution found by Lemos. Moreover, the dRGT black string solution includes other classes of black string solution such as the monopole-black string ones since the graviton mass contributes to the global monopole term as well as the cosmological-constant term. To investigate the solution, we compute mass, temperature, and entropy of the dRGT black string. We found that the existence of the graviton mass drastically affects the thermodynamics of the black string. Furthermore, the Hawking-Page phase transition is found to be possible for the dRGT black string as well as the charged dRGT black string. The dRGT black string solution is thermodynamically stable for r>r_c with negative thermodynamical potential and positive heat capacity while it is unstable for r

Black Music: Sound and Feeling for Black Liberation

ERIC Educational Resources Information Center

McClendon, William H.

1976-01-01

Focuses on contemporary black music and the assortment of persons who produce it noting that black music is one area where black people provide their definitions and make their own judgements. (Author/AM)

A Dancing Black Hole

NASA Astrophysics Data System (ADS)

Shoemaker, Deirdre; Smith, Kenneth; Schnetter, Erik; Fiske, David; Laguna, Pablo; Pullin, Jorge

2002-04-01

Recently, stationary black holes have been successfully simulated for up to times of approximately 600-1000M, where M is the mass of the black hole. Considering that the expected burst of gravitational radiation from a binary black hole merger would last approximately 200-500M, black hole codes are approaching the point where simulations of mergers may be feasible. We will present two types of simulations of single black holes obtained with a code based on the Baumgarte-Shapiro-Shibata-Nakamura formulation of the Einstein evolution equations. One type of simulations addresses the stability properties of stationary black hole evolutions. The second type of simulations demonstrates the ability of our code to move a black hole through the computational domain. This is accomplished by shifting the stationary black hole solution to a coordinate system in which the location of the black hole is time dependent.

Stability of squashed Kaluza-Klein black holes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kimura, Masashi; Ishihara, Hideki; Murata, Keiju

2008-03-15

The stability of squashed Kaluza-Klein black holes is studied. The squashed Kaluza-Klein black hole looks like a five-dimensional black hole in the vicinity of horizon and looks like a four-dimensional Minkowski spacetime with a circle at infinity. In this sense, squashed Kaluza-Klein black holes can be regarded as black holes in the Kaluza-Klein spacetimes. Using the symmetry of squashed Kaluza-Klein black holes, SU(2)xU(1){approx_equal}U(2), we obtain master equations for a part of the metric perturbations relevant to the stability. The analysis based on the master equations gives strong evidence for the stability of squashed Kaluza-Klein black holes. Hence, the squashed Kaluza-Kleinmore » black holes deserve to be taken seriously as realistic black holes in the Kaluza-Klein spacetime.« less

Cosmic microwave background radiation of black hole universe

NASA Astrophysics Data System (ADS)

Zhang, T. X.

2010-11-01

Modifying slightly the big bang theory, the author has recently developed a new cosmological model called black hole universe. This new cosmological model is consistent with the Mach principle, Einsteinian general theory of relativity, and observations of the universe. The origin, structure, evolution, and expansion of the black hole universe have been presented in the recent sequence of American Astronomical Society (AAS) meetings and published recently in a scientific journal: Progress in Physics. This paper explains the observed 2.725 K cosmic microwave background radiation of the black hole universe, which grew from a star-like black hole with several solar masses through a supermassive black hole with billions of solar masses to the present universe with hundred billion-trillions of solar masses. According to the black hole universe model, the observed cosmic microwave background radiation can be explained as the black body radiation of the black hole universe, which can be considered as an ideal black body. When a hot and dense star-like black hole accretes its ambient materials and merges with other black holes, it expands and cools down. A governing equation that expresses the possible thermal history of the black hole universe is derived from the Planck law of black body radiation and radiation energy conservation. The result obtained by solving the governing equation indicates that the radiation temperature of the present universe can be ˜2.725 K if the universe originated from a hot star-like black hole, and is therefore consistent with the observation of the cosmic microwave background radiation. A smaller or younger black hole universe usually cools down faster. The characteristics of the original star-like or supermassive black hole are not critical to the physical properties of the black hole universe at present, because matter and radiation are mainly from the outside space, i.e., the mother universe.

Affirmative action and the Black women in South Africa.

PubMed

Serote, P

1994-02-01

An overview was given of how affirmative action for Blacks and women in South Africa in fact marginalizes Black women. The definition of the problem influences the solution; affirmative action obscures the complex nature of discrimination experienced by Black women by class, role, and culture and focuses only on gender and race. Secondly, the power of White women and Black men supercede the power of Black women. Apartheid benefitted White women over Black men. Affirmative action, as shifting power between groups, would solidify White women's power. The debates have taken place within university and academic contexts, a place where Black women have been excluded and the dominant groups are White men, followed by White women, and then Black men. The debate in the private sector also is devoid of Black women's voices; multinationals began to hire and train Black male managers, and there was criticism that standards were falling. Recruitment of Black women is unknown, but only 1.1% of managers are Black. Visibility within the academic and private sector debates has excluded Black women. In the articulation of ideas, most literature has been written by White men. The intersection of power and privilege belongs to males and White women as part of the larger dominant ideology. Black women's marginalization means their issues will not be addressed. The people who stand to benefit the most from affirmative action are those who are in need of improved living conditions, literacy, and employment, or those excluded from jobs and position in spite of being qualified. Black women without a societal power base have no bargaining power. To insure that Black women benefit, there is need to treat Black women as a distinctive group with priority. There is also a need to examine the myths that have been spun around Black women, their needs, abilities, and controlling images. There is a need to integrate Black womens ideas into the mainstream and recognize that maybe Black women need to be recognized as already having some knowledge. Resistance to affirmative action needs to be stopped and the thrust of action focused on a liveable place for all. There is a need for multiple approaches which stress education, training, support systems, quotas, legislation, and an end to the bickering over which one works best.

Conformal Field Theory and black hole physics

NASA Astrophysics Data System (ADS)

Sidhu, Steve

2012-01-01

This thesis reviews the use of 2-dimensional conformal field theory applied to gravity, specifically calculating Bekenstein-Hawking entropy of black holes in (2+1) dimensions. A brief review of general relativity, Conformal Field Theory, energy extraction from black holes, and black hole thermodynamics will be given. The Cardy formula, which calculates the entropy of a black hole from the AdS/CFT duality, will be shown to calculate the correct Bekenstein-Hawking entropy of the static and rotating BTZ black holes. The first law of black hole thermodynamics of the static, rotating, and charged-rotating BTZ black holes will be verified.

On non-linear magnetic-charged black hole surrounded by quintessence

NASA Astrophysics Data System (ADS)

Nam, Cao H.

2018-06-01

We derive a non-linear magnetic-charged black hole surrounded by quintessence, which behaves asymptotically like the Schwarzschild black hole surrounded by quintessence but at the short distances like the dS geometry. The horizon properties of this black hole are investigated in detail. The thermodynamics of the black hole is studied in the local and global views. Finally, by calculating the heat capacity and the free energy, we point to that the black hole may undergo a thermal phase transition, between a larger unstable black hole and a smaller stable black hole, at a critical temperature.

Performance evaluation of two black nickel and two black chrome solar collectors

NASA Technical Reports Server (NTRS)

Losey, R.

1977-01-01

The test program was based on the evaluation of four unique solar collectors described below: (1) black nickel collector surface with a desiccant drying bed, (2) black nickel collector surface without a desiccant drying bed, (3) black chrome collector surface with a dessicant drying bed, and (4) black chrome collector surface without a desiccant drying bed. The test program included three distinct phases: Initial performance evaluation, natural environmental aging, and post-aging performance evaluation. Results of Phase III testing conclusively indicated a higher normalized efficiency for Black Chrome surfaces when compared to Black Nickel.

Familial Influences on Poverty Among Young Children in Black Immigrant, U.S.-born Black, and Nonblack Immigrant Families

PubMed Central

Thomas, Kevin J. A.

2014-01-01

This study examines how familial contexts affect poverty disparities between the children of immigrant and U.S.-born blacks, and among black and nonblack children of immigrants. Despite lower gross child poverty rates in immigrant than in U.S.-born black families, accounting for differences in family structure reveals that child poverty risks among blacks are highest in single-parent black immigrant families. In addition, within two-parent immigrant families, child poverty declines associated with increasing assimilation are greater than the respective declines in single-parent families. The heads of black immigrant households have more schooling than those of native-black households. However, increased schooling has a weaker negative association with child poverty among the former than among the latter. In terms of racial disparities among the children of immigrants, poverty rates are higher among black than nonblack children. This black disadvantage is, however, driven by the outcomes of first-generation children of African and Hispanic-black immigrants. The results also show that although children in refugee families face elevated poverty risks, these risks are higher among black than among nonblack children of refugees. In addition, the poverty-reducing impact associated with having an English-proficient household head is about three times lower among black children of immigrants than among non-Hispanic white children of immigrants. PMID:21491186

Rhetorical Perspectivism of Black Congressmen upon the 1875 Civil Rights Bill.

ERIC Educational Resources Information Center

Haskins, William A.

In their new roles as congressmen after the Civil War, blacks, for the first time in American history, advanced views of civil rights that reflected black perspectives. One scheme for analyzing black rhetoric suggests that black congressmen did not share the perspective held by many whites--that blacks were inept individuals. Rather, the black…

Relation of Racial Identity Attitudes to Self-Actualization and Affective States of Black Students.

ERIC Educational Resources Information Center

Parham, Thomas A.; Helms, Janet E.

1985-01-01

The relation between identity attitudes, or Black self-ac4ualization, and various affective states were investigated in 166 Black university students. Both pro-White/anti-Black and pro-Black/anti-White attitudes were associated with greater personal distress. Awakening Black identity was positively related to self-actualization tendencies and…

Sex Stereotyping in a Selected Sample of Black American Adolescents.

ERIC Educational Resources Information Center

Wilson, Maurice T.; Koeske, Gary F.

The present study investigated three major hypotheses: (1) that black respondents' judgments of black targets would show greater uniformity than their judgments of white targets; (2) that, contrary to trends in white samples, black males would be perceived less positively than black females; and (3) that self-ratings for black males would be less…

The Black Teacher Project: How Racial Affinity Professional Development Sustains Black Teachers

ERIC Educational Resources Information Center

Mosely, Micia

2018-01-01

The Black Teacher Project (BTP) is an organization that supports, develops and sustains Black teachers for schools in the United States. The organization is building a Black teaching force that reflects the diversity and excellence of Black people in the United States. In our pilot year, BTP offered racial affinity-based professional development…

Black Political Socialization and Political Change: The Black Panther Party Platform as a "Model" of Radical Political Socialization

ERIC Educational Resources Information Center

McLemore, Leslie Burl

1975-01-01

Considers the potential impact of black revolutionary nationalism--cultural and military--upon the social patterns of black people, arguing that a racial, cultural, and military approach through black organizations with a highly developed system of communications holds the key to black salvation in America. (Author/JM)

Black Women in the Academy: The Secrets to Success and Achievement.

ERIC Educational Resources Information Center

Gregory, Sheila T.

This book presents selected historical data focusing on three aspects in the lives of Black women: resilience of Black women and their families; strengths of the Black church and community; and formidable gains made by Black women in the workforce. The status and achievements of Black women professors and scholars in the academy are reviewed, with…

The Black Family as Educator.

ERIC Educational Resources Information Center

Wallace, Joan

The black family is the primary socializing agent of the black child and, thus, the primary educator. The culture of blacks in America, in which the child is steeped, is unique, complex and rich-the result of a convergence and fusion of African, American, and European influences. In its education of the black child, the black family must deal,…

Black Hole Safari: Tracking Populations and Hunting Big Game

NASA Astrophysics Data System (ADS)

McConnell, N. J.

2013-10-01

Understanding the physical connection, or lack thereof, between the growth of galaxies and supermassive black holes is a key challenge in extragalactic astronomy. Dynamical studies of nearby galaxies are building a census of black hole masses across a broad range of galaxy types and uncovering statistical correlations between galaxy bulge properties and black hole masses. These local correlations provide a baseline for studying galaxies and black holes at higher redshifts. Recent measurements have probed the extremes of the supermassive black hole population and introduced surprises that challenge simple models of black hole and galaxy co-evolution. Future advances in the quality and quantity of dynamical black hole mass measurements will shed light upon the growth of massive galaxies and black holes in different cosmic environments.

Thermodynamics of a class of regular black holes with a generalized uncertainty principle

NASA Astrophysics Data System (ADS)

Maluf, R. V.; Neves, Juliano C. S.

2018-05-01

In this article, we present a study on thermodynamics of a class of regular black holes. Such a class includes Bardeen and Hayward regular black holes. We obtained thermodynamic quantities like the Hawking temperature, entropy, and heat capacity for the entire class. As part of an effort to indicate some physical observable to distinguish regular black holes from singular black holes, we suggest that regular black holes are colder than singular black holes. Besides, contrary to the Schwarzschild black hole, that class of regular black holes may be thermodynamically stable. From a generalized uncertainty principle, we also obtained the quantum-corrected thermodynamics for the studied class. Such quantum corrections provide a logarithmic term for the quantum-corrected entropy.

"Iron-Clad" Evidence For Spinning Black Hole

NASA Astrophysics Data System (ADS)

2003-09-01

Telltale X-rays from iron may reveal if black holes are spinning or not, according to astronomers using NASA's Chandra X-ray Observatory and the European Space Agency's XMM-Newton Observatory. The gas flows and bizarre gravitational effects observed near stellar black holes are similar to those seen around supermassive black holes. Stellar black holes, in effect, are convenient `scale models' of their much larger cousins. Black holes come in at least two different sizes. Stellar black holes are between five and 20 times the mass of the Sun. At the other end of the size scale, supermassive black holes contain millions or billions times the mass of our Sun. The Milky Way contains both a supermassive black hole at its center, as well as a number of stellar black holes sprinkled throughout the Galaxy. At a press conference at the "Four Years of Chandra" symposium in Huntsville, Ala., Jon Miller of the Harvard-Smithsonian Center for Astrophysics in Cambridge, Mass. discussed recent results on the X-ray spectra, or distribution of X-rays with energy, from the iron atoms in gas around three stellar black holes in the Milky Way. "Discovering the high degree of correspondence between stellar and supermassive black holes is a real breakthrough," said Miller. "Because stellar black holes are smaller, everything happens about a million times faster, so they can be used as a test-bed for theories of how spinning black holes affect the space and matter around them." X-rays from a stellar black hole are produced when gas from a nearby companion star is heated to tens of millions of degrees as it swirls toward the black hole. Iron atoms in this gas produce distinctive X-ray signals that can be used to study the orbits of particles around the black hole. For example, the gravity of a black hole can shift the X-rays to lower energies. "The latest work provides the most precise measurements yet of the X-ray spectra for stellar black holes," said Miller. "These data help rule out competing explanations that do not require extreme gravitational effects, and provide the best look yet at the geometry of the space-time around a stellar black hole created by the death of a massive star." The orbit of a particle near a black hole depends on the curvature of space around the black hole, which also depends on how fast the black hole is spinning. A spinning black hole drags space around with it and allows atoms to orbit closer to the black hole than is possible for a non-spinning black hole. The latest Chandra data from Cygnus X-1, the first stellar-size black hole discovered, show that the gravitational effects on the signal from the iron atoms can only be due to relativistic effects, and that some of the atoms are no closer than 100 miles to the black hole. There was no evidence that the Cygnus X-1 black hole is spinning. The XMM-Newton data from the black hole, XTE J1650-500, show a very similar distribution of iron atom X-rays with one important exception. More low energy X-rays from iron atoms are observed, an indication that some X-rays are coming from deep in the gravitational well around the black hole, as close as 20 miles to the black hole event horizon. This black hole must be spinning rapidly. Chandra observations of a third stellar black hole, GX 339-4, have revealed that it is also spinning rapidly, and clouds of warm absorbing gas appear to be flowing away from the black hole at speeds of about three hundred thousand miles per hour. Such warm gas flows have been observed in the vicinity of supermassive black holes. Previous observations of some supermassive black holes by Japan's ASCA satellite, XMM-Newton and Chandra have indicated that they may also be rotating rapidly. The latest results presented by Miller indicate that the peculiar geometry of space around spinning stellar-mass black holes and supermassive black holes is remarkably similar. Stellar and supermassive black holes may be similar in other ways. Powerful jets of high-energy particles have been detected around both types of black holes. Why do some stellar black holes spin rapidly and others not? One possibility is that differences in spin are imparted at birth when a massive star collapses. Another possibility is that the spin rate depends on how long the black hole has been devouring matter from its companion star, a process that makes the black hole spin faster. Black holes with more rapid spin, XTE J1650-500 and GX 339-4, have low-mass companion stars. These relatively long-lived stars may have been feeding the black hole for longer, allowing it to spin up to faster rates. Cygnus X-1 with its short-lived companion star may not have not time to spin up. Miller is a National Science Foundation Astronomy & Astrophysics Postdoctoral Fellow. His primary collaborators in this work were Walter Lewin if the Massachusetts Institute of Technology in Cambridge, Andrew Fabian of the University of Cambridge, UK, and Chris Reynolds of the University of Maryland, College Park. NASA's Marshall Space Flight Center, Huntsville, Ala., manages the Chandra program for the Office of Space Science, NASA Headquarters, Washington. Northrop Grumman of Redondo Beach, Calif., formerly TRW, Inc., was the prime development contractor for the observatory. The Smithsonian Astrophysical Observatory controls science and flight operations from the Chandra X-ray Center in Cambridge, Mass.

Gamma ray bursts of black hole universe

NASA Astrophysics Data System (ADS)

Zhang, T. X.

2015-07-01

Slightly modifying the standard big bang theory, Zhang recently developed a new cosmological model called black hole universe, which has only a single postulate but is consistent with Mach's principle, governed by Einstein's general theory of relativity, and able to explain existing observations of the universe. In the previous studies, we have explained the origin, structure, evolution, expansion, cosmic microwave background radiation, quasar, and acceleration of black hole universe, which grew from a star-like black hole with several solar masses through a supermassive black hole with billions of solar masses to the present state with hundred billion-trillions of solar masses by accreting ambient matter and merging with other black holes. This study investigates gamma ray bursts of black hole universe and provides an alternative explanation for the energy and spectrum measurements of gamma ray bursts according to the black hole universe model. The results indicate that gamma ray bursts can be understood as emissions of dynamic star-like black holes. A black hole, when it accretes its star or merges with another black hole, becomes dynamic. A dynamic black hole has a broken event horizon and thus cannot hold the inside hot (or high-frequency) blackbody radiation, which flows or leaks out and produces a GRB. A star when it collapses into its core black hole produces a long GRB and releases the gravitational potential energy of the star as gamma rays. A black hole that merges with another black hole produces a short GRB and releases a part of their blackbody radiation as gamma rays. The amount of energy obtained from the emissions of dynamic star-like black holes are consistent with the measurements of energy from GRBs. The GRB energy spectra derived from this new emission mechanism are also consistent with the measurements.

The Black dentist workforce in the United States.

PubMed

Mertz, Elizabeth; Calvo, Jean; Wides, Cynthia; Gates, Paul

2017-03-01

The purpose of this paper is to describe the Black dentist workforce, the practice patterns of providers, and their contributions to oral health care for minority and underserved patients. A national sample survey of underrepresented minority dentists was conducted in 2012 and received a 32.6 percent response rate for self-reported Black dentists. Data were weighted for selection and response bias to be nationally representative. Descriptive and multivariable statistics were computed to provide a workforce profile of Black dentists. National comparisons are provided from published data. Among all Black dentists (weighted n = 6,254), 76.6 percent self-identify as African-American, 13.2 percent as African, and 10.3 percent as Afro-Caribbean. The largest share of Black dentists are male, married, heterosexual, born in the United States and raised in a medium to large city. One third of Black dentists were the first in their family to graduate from college. Black dentists report higher average educational debt than all dental students, with graduates from International Dentist Programs having the greatest debt. Traditional practices (i.e., private practices) dominate, with 67.1 percent of Black dentists starting out in this setting and 73.5 percent currently in the setting. Black dentists care for a disproportionate share of Black patients, with an average patient mix that is 44.9 percent Black. Two in five Black dentists reported their patient pool is made up of more than 50 percent Black patients. The underrepresentation for Black dentists is extraordinary, and the Black dentists that are in practice are shouldering a disproportionate share of dental care for minority and underserved communities. © 2016 American Association of Public Health Dentistry.

The origin of black carbon on speleothems in tourist caves in South Korea: Chemical characterization and source discrimination by radiocarbon measurement

NASA Astrophysics Data System (ADS)

Chang, Sae Jung; Jeong, Gi Young; Kim, Soo Jin

Since the Gosu, Ondal, and Sungryu karst caves in South Korea became open to the public several decades ago, the surface of their speleothems has been turning black due to pollutants. The black pollutant is concentrated at the surface of speleothems, and the surface black layer is 0.1 to several millimeters thick. Elemental measurements of three bulk, acid-dissolved and oxidized fractions of the surface black layer show that the black pigment is a black carbon. The black carbon correlates positively with sulfates, nitrates, manganese, and lead, which are typical tracers of industrial and urban emissions. The 14C-measurement of the black carbon, using accelerator mass spectrometry, shows that the black carbon was derived from both fossil-fuel combustion and biomass burning in roughly equal amounts, evidenced by fC value ranging from 0.340 to 0.592 (<±0.004, 1 σ). Therefore, protection of speleothems from black coloration requires control of anthropogenic black carbons carried by visitors. Suitable measures might include closure of caves, air cleaning of visitors and regulation of visitor numbers. The application of radiocarbon measurement of black carbon suggests that the fC is a valuable proxy for tracing the blackening phenomenon of natural and cultural heritage sites such as caves.

REPORT TO CONGRESS ON BLACK CARBON

EPA Science Inventory

The Report to Congress on Black Carbon describes domestic and international sources of black carbon emissions, and summarizes available scientific information on the climate effects of black carbon. Further, the Report evaluates available black carbon mitigation options and thei...

Celebration of Black Composers: Black Music at the Philharmonic

ERIC Educational Resources Information Center

Current, Gloster B.

1978-01-01

The five-day celebration of black composers at the New York Philharmonic included a panel discussion of black music and three symphonic concerts featuring the premieres of new works by celebrated black composers. (Author/AM)

Pyrolytic carbon coated black silicon

NASA Astrophysics Data System (ADS)

Shah, Ali; Stenberg, Petri; Karvonen, Lasse; Ali, Rizwan; Honkanen, Seppo; Lipsanen, Harri; Peyghambarian, N.; Kuittinen, Markku; Svirko, Yuri; Kaplas, Tommi

2016-05-01

Carbon is the most well-known black material in the history of man. Throughout the centuries, carbon has been used as a black material for paintings, camouflage, and optics. Although, the techniques to make other black surfaces have evolved and become more sophisticated with time, carbon still remains one of the best black materials. Another well-known black surface is black silicon, reflecting less than 0.5% of incident light in visible spectral range but becomes a highly reflecting surface in wavelengths above 1000 nm. On the other hand, carbon absorbs at those and longer wavelengths. Thus, it is possible to combine black silicon with carbon to create an artificial material with very low reflectivity over a wide spectral range. Here we report our results on coating conformally black silicon substrate with amorphous pyrolytic carbon. We present a superior black surface with reflectance of light less than 0.5% in the spectral range of 350 nm to 2000 nm.

Black and brown pigment gallstones differ in microstructure and microcomposition.

PubMed

Malet, P F; Takabayashi, A; Trotman, B W; Soloway, R D; Weston, N E

1984-01-01

The two subtypes of pigment gallstones, black and brown stones, differ in chemical composition and pathogenesis. We examined a black bilirubinate stone and a black phosphate stone (which represented opposite ends of the compositional spectrum of black noncarbonate stones), a black carbonate stone, and a brown pigment stone using scanning electron microscopy and microchemical techniques to determine if stone microstructure and microcomposition reflected different patterns of formation. The cross-sectional surfaces of the black bilirubinate and black phosphate stones were smooth and homogenous. Electron probe microanalysis demonstrated high concentrations of sulfur and copper in the center of the black bilirubinate stone; sulfur was in a low valence state consistent with disulfide linkages in proteins. The brown stone was rough-surfaced with lamellated bands on cross-section. The lighter-colored bands in this stone contained virtually all of the detected calcium palmitate, while the darker sections contained much more calcium bilirubinate. Plasma oxygen etching demonstrated a network of protein interdigitating with calcium bilirubinate salts in the black bilirubinate and black phosphate stones but not in the black carbonate or brown stones. Argon ion etching demonstrated that calcium bilirubinate was in a closely packed rod-shaped arrangement in all three black stones but not in the brown stone. We conclude that the marked differences in structure and composition between the black noncarbonate and brown pigment gallstones support the hypothesis that the two major pigment gallstone types form by different mechanisms. In addition, the layered structures of the black carbonate and brown stones suggest that stone growth is affected by cyclic changes in biliary composition.

A black berry that is not a blackberry

USDA-ARS?s Scientific Manuscript database

Red raspberries and blackberries, unlike black raspberries, are widespread and familiar. Most consumers rarely encounter black raspberries; consequently, they frequently confuse fresh black raspberries with common cane berries. Recognition and appreciation of black raspberries can be improved with c...

Black Literature vs. Black Studies: Three Lynchings

ERIC Educational Resources Information Center

Williams, Melvin G.

1977-01-01

Considers three works by black authors, all dealing with lynchings, that may be used in a black literature course to introduce students to the esthetic dimension of black literature, as well as to its cultural and racial significance. (GW)

A Comparative Perspective of Black College Males on the Achievement Gap: Implications for School Counselors

ERIC Educational Resources Information Center

Bethell, Detra

2013-01-01

The dominant discourse regarding Black males in America is that they are less academically successful than European American males and Black females. Similarly, Black males in the Bahamas are described as less successful than Black Bahamian females. School Counselors are in a unique position to alter the trajectory of success for Black males if…

Locating Black Mixed-Raced Males in the Black Supplementary School Movement

ERIC Educational Resources Information Center

Joseph-Salisbury, Remi; Andrews, Kehinde

2017-01-01

This article draws upon data from semi-structured interviews conducted with black mixed-race males in the UK and the US, to argue that a revival of the black supplementary school movement could play an important role in the education of black mixed-race males. The article contends that a strong identification with blackness, and a concomitant…

"No Cosby Show": Single Black Mother Homes and How Black Men Build Romantic Relationships

ERIC Educational Resources Information Center

Moore, Maia Niguel

2016-01-01

This qualitative study explored the lives of 11 Black men to better understand how Black men who were raised in single Black mother homes build romantic relationships with Black women. One focus group and a series of individual in-person interviews were conducted with the participants who ranged between 23 and 43 years of age. Participants were…

Is Nursing a Viable Career for Blacks? (A Study of Black and White Freshman Nursing Students).

ERIC Educational Resources Information Center

Miller, Michael H.

It has been suggested that underrepresentation of blacks in professional nursing results from insufficient black-nurse role models. This study of 331 black and white freshman nursing students in three, two year, associate degree programs argues that blacks are not professional nurses for reasons other than a lack of role models. The results show…

Rotating black holes in dilatonic Einstein-Gauss-Bonnet theory.

PubMed

Kleihaus, Burkhard; Kunz, Jutta; Radu, Eugen

2011-04-15

We construct generalizations of the Kerr black holes by including higher-curvature corrections in the form of the Gauss-Bonnet density coupled to the dilaton. We show that the domain of existence of these Einstein-Gauss-Bonnet-dilaton (EGBD) black holes is bounded by the Kerr black holes, the critical EGBD black holes, and the singular extremal EGBD solutions. The angular momentum of the EGBD black holes can exceed the Kerr bound. The EGBD black holes satisfy a generalized Smarr relation. We also compare their innermost stable circular orbits with those of the Kerr black holes and show the existence of differences which might be observable in astrophysical systems.

[Effects of different rootstocks on the weak light tolerance ability of summer black grape based on 4 photo-response models].

PubMed

Han, Xiao; Wang, Hai Bo; Wang, Xiao di; Shi, Xiang Bin; Wang, Bao Liang; Zheng, Xiao Cui; Wang, Zhi Qiang; Liu, Feng Zhi

2017-10-01

The photo response curves of 11 rootstock-scion combinations including summer black/Beta, summer black/1103P, summer black/101-14, summer black/3309C, summer black/140Ru, summer black/5C, summer black/5BB, summer black/420A, summer black/SO4, summer black/Kangzhen No.1, summer black/Huapu No.1 were fitted by rectangular hyperbola mo-del, non-rectangular hyperbola model, modified rectangular hyperbola model and exponential model respectively, and the differences of imitative effects were analyzed by determination coefficiency, light compensation point, light saturation point, initial quantum efficiency, maximum photosynthetic rate and dark respiration rate. The result showed that the fit coefficients of all four models were above 0.98, and there was no obvious difference on the fitted values of light compensation point among the four models. The modified rectangular hyperbola model fitted best on light saturation point, apparent quantum yield, maximum photosynthetic rate and dark respiration rate, and had the minimum AIC value based on the akaike information criterion, therefore, the modified rectangular hyperbola model was the best one. The clustering analysis indicated that summer black/SO4 and summer black/420A combinations had low light compensation point, high apparent quantum yield and low dark respiration rate among 11 rootstock-scion combinations, suggesting that these two combinations could use weak light more efficiently due to their less respiratory consumption and higher weak light tolerance. The Topsis comparison method ranked summer black/SO4 and summer black/420A combinations as No. 1 and No. 2 respectively in weak light tolerance ability, which was consistent with cluster analysis. Consequently, summer black has the highest weak light tolerance in case grafted on 420A or SO4, which could be the most suitable rootstock-scion combinations for protected cultivation.

Revisit on the thermodynamic stability of Hořava-Lifshitz black hole

NASA Astrophysics Data System (ADS)

Meng, Xudong; Wang, Ruihong

We study the thermodynamic properties of the black hole derived in Hořava-Lifshitz (HL) gravity without the detailed-balance condition. The parameter Ξ = 𝜖2 in the HL black hole plays the same role as that of the electric charge in the Reissner-Nordström-anti-de Sitter (RN-AdS) black hole. By analogy, we treat the parameter Ξ as the thermodynamic variable and obtain the first law of thermodynamics for the HL black hole. Although the HL black hole and the RN-AdS black hole have the similar mass and temperature, due to their very different entropy, the two black holes have very different thermodynamic properties. By calculating the heat capacity and the free energy, we analyze the thermodynamic stability of the HL black hole.

Supersymmetric black holes and Freudenthal duality

NASA Astrophysics Data System (ADS)

Marrani, Alessio; Mandal, Taniya; Tripathy, Prasanta K.

2017-07-01

We study the effect of Freudenthal duality on supersymmetric extremal black hole attractors in 𝒩 = 2, D = 4 ungauged supergravity. Freudenthal duality acts on the dyonic black hole charges as an anti-involution which keeps the black hole entropy and the critical points of the effective black hole potential invariant. We analyze its effect on the recently discovered distinct, mutually exclusive phases of axionic supersymmetric black holes, related to the existence of nontrivial involutory constant matrices. In particular, we consider a supersymmetric D0 - D4 - D6 black hole and we explicitly Freudenthal-map it to a supersymmetric D0 - D2 - D4 - D6 black hole. We thus show that the charge representation space of a supersymmetric D0 - D2 - D4 - D6 black hole also contains mutually exclusive domains.

Thermodynamic and classical instability of AdS black holes in fourth-order gravity

NASA Astrophysics Data System (ADS)

Myung, Yun Soo; Moon, Taeyoon

2014-04-01

We study thermodynamic and classical instability of AdS black holes in fourth-order gravity. These include the BTZ black hole in new massive gravity, Schwarzschild-AdS black hole, and higher-dimensional AdS black holes in fourth-order gravity. All thermo-dynamic quantities which are computed using the Abbot-Deser-Tekin method are used to study thermodynamic instability of AdS black holes. On the other hand, we investigate the s-mode Gregory-Laflamme instability of the massive graviton propagating around the AdS black holes. We establish the connection between the thermodynamic instability and the GL instability of AdS black holes in fourth-order gravity. This shows that the Gubser-Mitra conjecture holds for AdS black holes found from fourth-order gravity.

Perturbations of the Kerr black hole and the boundness of linear waves

NASA Astrophysics Data System (ADS)

Eskin, G.

2010-11-01

Artificial black holes (also called acoustic or optical black holes) are the black holes for the linear wave equation describing the wave propagation in a moving medium. They attracted a considerable interest of physicists who study them to better understand the black holes in general relativity. We consider the case of stationary axisymmetric metrics and we show that the Kerr black hole is not stable under perturbations in the class of all axisymmetric metrics. We describe families of axisymmetric metrics having black holes that are the perturbations of the Kerr black hole. We also show that the ergosphere can be determined by boundary measurements. Finally, we prove the uniform boundness of the solution in the exterior of the black hole when the event horizon coincides with the ergosphere.

Black Pete through the Eyes of Dutch Children

PubMed Central

Mesman, Judi; Janssen, Sofie; van Rosmalen, Lenny

2016-01-01

The traditional figure of Black Pete seen during the December festivities around Sinterklaas (the Dutch Santa Claus) in the Netherlands has sparked fierce debates about his racial stereotypical characteristics and his potentially negative effects on children’s opinions about black people. The Black Pete phenomenon has even been discussed by the United Nations Committee on the Elimination of Racial Discrimination, resulting in a report urging the Netherlands to eliminate this form of racial stereotyping. The adult debate about Black Pete is clearly important, but Sinterklaas is essentially a children’s holiday. Surprisingly, there have never been any systematic studies to examine children’s views on Black Pete. The current study is the first to do so. In a sample of 201 children aged 5–7 years, we collected free descriptions of Black Pete, asked children to group him in relation to other figures, and to assign characteristics to him and comparison figures. The results showed that (1) Children are clearly aware of Black Pete’s skin color and subordinate status; (2) Children associate Black Pete more with clowns than with black people; (3) Children evaluate Black Pete very positively, but the positive characteristics do not generalize to their evaluation of black people. The findings illustrate the deep-rooted childhood origins of many Dutch people’s affection for Black Pete and their lack of awareness of his relation to racial stereotypes. This explains the resistance to changing the Black Pete figure and the slowness of the change process on this front. PMID:27322583

Black Hessians: American Blacks as German Soldiers.

ERIC Educational Resources Information Center

Hoffman, Elliott W.

1981-01-01

The German army in America during the Revolutionary War enlisted Blacks as musicians, laborers, and soldiers. Black soldiers contributed to the mercenaries' military activities, while the German units offered Blacks employment, clothing, food, and a type of escape from slavery. (Author/MJL)

Black Alcoholism.

ERIC Educational Resources Information Center

Watts, Thomas D.; Wright, Roosevelt

1988-01-01

Examines some aspects of the problem of alcoholism among Blacks, asserting that Black alcoholism can best be considered in an ecological, environmental, sociocultural, and public health context. Notes need for further research on alcoholism among Blacks and for action to reduce the problem of Black alcoholism. (NB)

Differences Between Black Youth who Support the Black Panthers and the NAACP.

ERIC Educational Resources Information Center

Levine, Daniel U.; And Others

The purpose of this study is to compare the attitudes of black youth who have a favorable orientation toward the Black Panthers with those of black youth who appear to be oriented toward the N.A.A.C.P. Information involving orientations toward organizations concerned with the lives of black Americans was collected as part of a larger study on the…

Black Theatre: Ideas That Matter in the Pursuit of Human Dignity.

ERIC Educational Resources Information Center

Sandle, Floyd L.

Black theatre has come a long way from the major stereotypes of black people established by white playwrights, but it is still trying to convey the fact that blacks remain in a kind of prison. The slow pace of black theatre in becoming art is obviously due to the long degradation of blacks, the tolerated injustice of whites, and the belated…

A Prime Assessment for Black Colleges: Role of Blacks in the Sciences and Related Fields.

ERIC Educational Resources Information Center

Branson, Herman

1978-01-01

This article examines the need for blacks in science and engineering. From a review of the history of blacks as objects of scientific study from the 1790s to the present a better grasp of the problems confronting blacks can be gained by examining two fields: genetics and psychology. In genetic research on blacks, the results are used not by…

Understanding the Invisibility of Black Nurse Leaders Using a Black Feminist Poststructuralist Framework.

PubMed

Jefferies, Keisha; Goldberg, Lisa; Aston, Megan; Murphy, Gail Tomblin

2018-05-12

This paper explores the invisibility and underrepresentation of Black nurses in formal and informal leadership roles using a Black feminist poststructuralist framework. The paper describes historical and contemporary challenges experienced by Black nurses throughout their nursing education and in practice. It also highlights how social and institutional discourses continue to marginalize and oppress Black nurses as leaders and render them invisible. Diversity amongst nursing leaders is essential to inform health care delivery, develop inclusive practices and provide culturally sensitive care. Despite this glaring need for diversity within nursing in Canada, there remains a significant underrepresentation of Black nurses in the workforce and as leaders. This is a discursive paper on Black nurses in nursing education and the workforce as well as their location as leaders in health care through a critical analysis using Black feminist poststructuralism. A review of the literature involved searching electronic databases CINAHL, NovaNet, PubMed and Google Scholar using keywords including: Black; African; Nurses; Leaders; Feminism; Poststructural. Articles were screened by titles and abstracts before accessing full-text for relevant articles. Black feminist poststructuralism uncovers how power, language, subjectivity and agency are constructed by the historically ingrained social and institutional discourses of everyday life for Black nurses. Experiences of discrimination and oppression were common throughout nursing education and practice for Black nurses, resulting in feelings of marginalization and isolation. The invisibility of Black nurse leaders is the result of generational oppression and discrimination manifested through discourses. Systemic, institutional and historical discourses perpetuate barriers for Black nurse leaders, resulting in their invisibility or absence in practice. This paper is designed to generate discussion related to the invisibility of Black nurse leaders by providing an understanding of the historical experiences of Blacks, their entry into the nursing profession and the present day challenges they face. This discussion will inform health care practice, policy, and structuring by identifying the barriers to leadership for Black nurses. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

A Sprinkle of Pepper: The State of Black Influence in White Colleges and Universities

ERIC Educational Resources Information Center

Hale, Frank W., Jr.

1975-01-01

The influence of Blacks in white institutions of higher education is no more than a sprinkling of pepper. The article discusses some problems facing the Black students, Black faculty and Black administrators at these schools. (Author/HMV)

40 CFR 458.40 - Applicability; description of the carbon black lamp process subcategory.

Code of Federal Regulations, 2011 CFR

2011-07-01

... carbon black lamp process subcategory. 458.40 Section 458.40 Protection of Environment ENVIRONMENTAL... CATEGORY Carbon Black Lamp Process Subcategory § 458.40 Applicability; description of the carbon black lamp... production of carbon black by the lamp process. ...

Land of Promise, Cities of Despair: Blacks in Urban America.

ERIC Educational Resources Information Center

Staples, Robert

1978-01-01

This article discusses the current effect on Blacks of unequal political and economic power in American cities. Some of the issues examined include: Black migration; employment status; education; socioeconomic status; Black suicide; Black political power; housing; health; and racism. (EB)

Fermions tunnelling from the charged dilatonic black holes

NASA Astrophysics Data System (ADS)

Chen, De-You; Jiang, Qing-Quan; Zu, Xiao-Tao

2008-10-01

Kerner and Mann's recent work shows that for an uncharged and non-rotating black hole its Hawking temperature can be correctly derived by fermions tunnelling from its horizons. In this paper, our main work is to improve the analysis to deal with charged fermion tunnelling from the general dilatonic black holes, specifically including the charged, spherically symmetric dilatonic black hole, the rotating Einstein Maxwell dilaton axion (EMDA) black hole and the rotating Kaluza Klein (KK) black hole. As a result, the correct Hawking temperatures are well recovered by charged fermions tunnelling from these black holes.

Throat quantization of the Schwarzschild-Tangherlini(-AdS) black hole

NASA Astrophysics Data System (ADS)

Maeda, Hideki

2018-01-01

By the throat quantization pioneered by Louko and Mäkelä, we derive the mass and area/entropy spectra for the Schwarzschild-Tangherlini-type asymptotically flat or AdS vacuum black hole in arbitrary dimensions. Using the WKB approximation for black holes with large mass, we show that area/entropy is equally spaced for asymptotically flat black holes, while mass is equally spaced for asymptotically AdS black holes. Exact spectra can be obtained for toroidal AdS black holes in arbitrary dimensions including the three-dimensional BTZ black hole.

Black holes of dimensionally continued gravity coupled to Born-Infeld electromagnetic field

NASA Astrophysics Data System (ADS)

Meng, Kun; Yang, Da-Bao

2018-05-01

In this paper, for dimensionally continued gravity coupled to Born-Infeld electromagnetic field, we construct topological black holes in diverse dimensions and construct dyonic black holes in general even dimensions. We study thermodynamics of the black holes and obtain first laws. We study thermal phase transitions of the black holes in T-S plane and find van der Waals-like phase transitions for even-dimensional spherical black holes, such phase transitions are not found for other types of black holes constructed in this paper.

Substantial Family History of Prostate Cancer in Black Men Recruited for Prostate Cancer Screening

PubMed Central

Mastalski, Kathleen; Coups, Elliot J.; Ruth, Karen; Raysor, Susan; Giri, Veda N.

2008-01-01

Background Black men are at increased risk for prostate cancer (PCA), particularly with a family history (FH) of the disease. Previous reports have raised concern for suboptimal screening of Black men with a FH of PCA. We report on the extent of FH of PCA from a prospective, longitudinal PCA screening program for high-risk men. Methods Black men ages 35-69 are eligible for PCA screening through the Prostate Cancer Risk Assessment Program (PRAP) regardless of FH. Rates of self-reported FH of PCA, breast, and colon cancer at baseline were compared with an age-matched sample of Black men from the 2005 National Health Interview Survey (NHIS) using standard statistical methods. Results As of January 2007, 332 Black men with pedigree information were enrolled in PRAP and FH of PCA was compared to 838 Black men from the 2005 NHIS. Black men in PRAP reported significantly more first-degree relatives with PCA compared to Black men in the 2005 NHIS (34.3%, 95% CI 29.2-39.7 vs. 5.7%, 95% CI 3.9-7.4). Black men in PRAP also had more FH of breast cancer compared to the 2005 NHIS (11.5%, 95% CI 8.2-15.4 vs 6.3%, 95% CI 4.6-8.0). Conclusions FH of PCA appears to be a motivating factor for Black men seeking PCA screening. Targeted recruitment and education among Black families should improve PCA screening rates. Efforts to recruit Black men without a FH of PCA are also needed. Condensed Abstract Black men seeking prostate cancer screening have a substantial burden of family history of prostate cancer. Targeted education and enhancing discussion in Black families should increase prostate cancer screening and adherence. PMID:18816608

A Comparison of Birth Outcomes Among Black, Hispanic, and Black Hispanic Women

PubMed Central

BeLue, Rhonda; Hillemeier, Marianne M.

2015-01-01

Background While non-Hispanic Black populations tend to be disproportionately affected by adverse reproductive outcomes, Hispanic populations tend to demonstrate healthier birth outcomes, regardless of socioeconomic background. Little is known about birth outcomes for women who are both Black and Hispanic. We examined whether birth outcomes and risk factors for women who are both Black and Hispanic most closely resemble those of women who are only Black or Hispanic and also compared these outcomes to those for Whites. Methods Using the 2013 US natality files, we examined 2,970,315 singleton births to Black Hispanic, Hispanic, Black, and White mothers. We used logistic regression to calculate predicted probabilities of low birth weight (LBW), preterm birth (PTB), or small for gestational age (SGA). Race-stratified regression analysis was used to identify the factors that significantly predicted risk for each outcome for each racial/ethnic group. Results Black mothers had the highest prevalence and predicted probabilities of experiencing all three outcomes. Black Hispanic mothers were less likely than Black mothers and more likely than Hispanic mothers to experience each of the adverse outcomes. We also found support for racial variation in risk and protective factors for mothers in the different groups. Factors like age and education inconsistently predicted risk of experiencing the birth outcomes for all groups. Overall, Black Hispanic mothers had birth outcomes and risk factor profiles like Hispanic mothers, although they had sociodemographic characteristics and health behaviors like Black mothers. Conclusions Patterning of birth outcomes among Black Hispanic women suggest an intersection of risk and protective factors associated with their respective racial and ethnic identities. Additional information about sociodemographic context is needed to develop a more complete picture of how factors related to race and ethnic group membership influence Black Hispanic women’s birth outcomes. PMID:26561541

The specter of the black fag: parody, blackness, and hetero/homosexual B(r)others.

PubMed

Johnson, E Patrick

2003-01-01

This essay investigates the ways in which three African American heterosexual males-Eddie Murphy, Damon Wayans, and David Alan Grier-appropriate signifiers of gayness to parody, stereotype, and repudiate black gay men. These performances are also attempts to circumscribe the boundaries of blackness, ultimately suggesting that "authentic" blackness is lodged within hegemonic black masculinity. Contrary to this desired effect, the essay demonstrates how these performers, in the act of repudiation, ironically and unwittingly queer heteronormative black masculinity, securing further the dialectic between heterosexuality and homosexuality. Finally, the essay argues that these performances manifest the black heterosexual male's melancholia, his refusal to grieve the loss of his sexual B(r)other.

Black Male Mental Health and the Black Church: Advancing a Collaborative Partnership and Research Agenda.

PubMed

Robinson, Michael A; Jones-Eversley, Sharon; Moore, Sharon E; Ravenell, Joseph; Adedoyin, A Christson

2018-06-01

This article explores the role the Black Church could play in facilitating spiritually sensitive, culturally relevant and gender-specific services to address the mental health and well-being of Black males. The help-seeking behaviors of Black men are examined as the authors offer two theories: the body, mind, spirit, environment, social, transcendent, and health, illness, men, and masculinities that may assist the Black Church in functioning as an effective support networks for healthy Black male mental health. Next, the authors discuss implications for practice, research, and education, and lastly, eight recommendations for Black Church leadership, social workers, and mental health professionals are also discussed.

World-volume effective theory for higher-dimensional black holes.

PubMed

Emparan, Roberto; Harmark, Troels; Niarchos, Vasilis; Obers, Niels A

2009-05-15

We argue that the main feature behind novel properties of higher-dimensional black holes, compared to four-dimensional ones, is that their horizons can have two characteristic lengths of very different size. We develop a long-distance world-volume effective theory that captures the black hole dynamics at scales much larger than the short scale. In this limit the black hole is regarded as a blackfold: a black brane (possibly boosted locally) whose world volume spans a curved submanifold of the spacetime. This approach reveals black objects with novel horizon geometries and topologies more complex than the black ring, but more generally it provides a new organizing framework for the dynamics of higher-dimensional black holes.

Population ecology and harvest of the American black duck: a review

USGS Publications Warehouse

Rusch, D.H.; Ankney, C.D.; Boyd, H.; Longcore, J.R.; Montalbano, F.; Ringelman, J.K.; Stotts, V.D.

1989-01-01

1. The purpose of our review was to examine available data on population trends and current status of black ducks and trends in natality and survival and to relate these, where possible, to changes in habitat, predation, disease, contaminants, harvest, and hybridization with mallards. 2. The number of black ducks tallied in the winter survey has declined steadily over the past 30 years at an average rate of about 3%/ year. Reliability and precision of the survey are uncertain; it may not provide an adequate index to the continental population of black ducks. Breeding surveys are incomplete and sporadic, but black ducks have decreased in Ontario and increased in the Maritime Provinces and Quebec. 3. Recent declines in numbers of black ducks tallied in the winter survey are not unusual in magnitude or much different from those that have occurred among several other species of waterfowl. 4. At present, black ducks are not especially scarce relative to numbers of several other ducks in eastern North America. 5. There is no solid evidence of major decreases in quality or quantity of breeding habitat for black ducks in recent years; in some areas, habitat has improved. 6. Natural mortality of black ducks has not been well studied, but does not seem unusually high compared to other dabbling ducks. 7. Harvest rates of black ducks are similar to those of sympatric mallards as determined by banding analyses. 8. There is no strong evidence for direct effects of contaminants on black ducks, but some indirect effects through invertebrate food resources have been detected. 9. Age ratios in black ducks show no trend in the past 18 years. 10. The quality and quantity of wintering habitat for black ducks have decreased substantially in some areas. 11. Disease and other natural mortality that affect black ducks do .not occur in unusually high frequency. 12. A decline in harvest of black ducks has occurred; most of the decline has been in the United States, especially since restrictive regulations were implemented in 1983. 13. Recovery rates of black ducks have declined recently in the U.S., but not in Canada. 14. Survival rates of black ducks are 56-63% for adults and 43% for young. These rates of survival are similar to comparable estimates in sympatric mallards. 15. Long hunting seasons may depress survival in some sex-age classes of black ducks, buteffects of small reductions in survival on population trends are unknown. 16. Available evidence does not support the contention that hunting is either the sole or most important cause of the decline in the winter tally of black duck numbers. 17. Surveys and banding of black ducks should be thoroughly reviewed and maintained or improved as warranted. Obtaining or maintaining a reasonable index to numbers of black ducks is the top priority among survey needs. 18. Experimental manipulation of hunting seasons should be considered to elucidate relationships among regulations, harvest, survival, and population trends. 19. Black ducks and mallards are genetically similar; there is as much genetic differentiation within the 2 species as there is between them. 20. Black duck x mallard hybrids are fertile. Hybrids are difficult to detect by plumage and thus published frequencies (0-13%) of hybrids may be low. 21. Hybridization could be a result of concomitant mallard increases and black duck decreases, or changes in black duck-to-mallard ratios could be from hybridization and genetic swamping of black ducks.

BlackMax: A black-hole event generator with rotation, recoil, split branes, and brane tension

NASA Astrophysics Data System (ADS)

Dai, De-Chang; Starkman, Glenn; Stojkovic, Dejan; Issever, Cigdem; Rizvi, Eram; Tseng, Jeff

2008-04-01

We present a comprehensive black-hole event generator, BlackMax, which simulates the experimental signatures of microscopic and Planckian black-hole production and evolution at the LHC in the context of brane world models with low-scale quantum gravity. The generator is based on phenomenologically realistic models free of serious problems that plague low-scale gravity, thus offering more realistic predictions for hadron-hadron colliders. The generator includes all of the black-hole gray-body factors known to date and incorporates the effects of black-hole rotation, splitting between the fermions, nonzero brane tension, and black-hole recoil due to Hawking radiation (although not all simultaneously). The generator can be interfaced with Herwig and Pythia. The main code can be downloaded from http://www-pnp.physics.ox.ac.uk/~issever/BlackMax/blackmax.html.

Black Hole in 3-D

NASA Image and Video Library

1999-11-30

This three-dimensional illustration shows how the rotating space around a black hole twists up the magnetic field in the plasma falling toward the black hole. The black sphere at the center of the figure is the black hole itself. http://photojournal.jpl.nasa.gov/catalog/PIA04207

Intergenerational Mobility and Goal-Striving Stress Among Black Americans: The Roles of Ethnicity and Nativity Status.

PubMed

Mouzon, Dawne M; Watkins, Daphne C; Perry, Ramona; Simpson, Theresa M; Mitchell, Jamie A

2018-04-02

Goal-striving stress refers to the psychological consequences of seeking but failing to reach upward mobility and is more common among low-income and people of color. Intergenerational mobility-or improved socioeconomic standing relative to one's parents-may be an important predictor of goal-striving stress for Blacks. We used the National Survey of American Life to investigate the association between intergenerational mobility and goal-striving stress among U.S.-born African Americans, U.S.-born Caribbean Blacks, and foreign-born Caribbean Blacks. Intergenerational mobility was associated with lower goal-striving stress and U.S.-born African Americans and Caribbean Blacks reported lower goal-striving stress than foreign-born Caribbean Blacks. Goal-striving stress was relatively high among foreign-born Blacks, regardless of level of intergenerational mobility attained. Goal-striving is an important stressor for foreign-born Caribbean Blacks, regardless of their level of educational success. Given increasing Black migration, future studies should disaggregate the Black racial category based on ethnicity and nativity.

The black gender gap in educational attainment: historical trends and racial comparisons.

PubMed

McDaniel, Anne; DiPrete, Thomas A; Buchmann, Claudia; Shwed, Uri

2011-08-01

It is often asserted that the gender gap in educational attainment is larger for blacks than whites, but historical trends comparing the black and white gender gap have received surprisingly little attention. Analysis of historical data from the U.S. census IPUMS samples shows that the gender gap in college completion has evolved differently for whites and blacks. Historically, the female advantage in educational attainment among blacks is linked to more favorable labor market opportunities and stronger incentives for employment for educated black women. Blacks, particularly black males, still lag far behind whites in their rates of college completion, but the striking educational gains of white women have caused the racial patterns of gender differences in college completion rates to grow more similar over time. While some have linked the disadvantaged position of black males to their high risk of incarceration, our estimates suggest that incarceration has a relatively small impact on the black gender gap and the racial gap in college completion rates for males in the United States.

Entropy is conserved in Hawking radiation as tunneling: A revisit of the black hole information loss paradox

NASA Astrophysics Data System (ADS)

Zhang, Baocheng; Cai, Qing-yu; Zhan, Ming-sheng; You, Li

2011-02-01

We revisit in detail the paradox of black hole information loss due to Hawking radiation as tunneling. We compute the amount of information encoded in correlations among Hawking radiations for a variety of black holes, including the Schwarzchild black hole, the Reissner-Nordström black hole, the Kerr black hole, and the Kerr-Newman black hole. The special case of tunneling through a quantum horizon is also considered. Within a phenomenological treatment based on the accepted emission probability spectrum from a black hole, we find that information is leaked out hidden in the correlations of Hawking radiation. The recovery of this previously unaccounted for information helps to conserve the total entropy of a system composed of a black hole plus its radiations. We thus conclude, irrespective of the microscopic picture for black hole collapsing, the associated radiation process: Hawking radiation as tunneling, is consistent with unitarity as required by quantum mechanics.

Electron Pair Repulsion Responsible for the Peculiar Edge Effects and Surface Chemistry of Black Phosphorus.

PubMed

Kong, Xiang-Peng; Shen, Xiaomei; Jang, Joonkyung; Gao, Xingfa

2018-03-01

The electronic and optical properties of black phosphorus (black-P) are significantly modulated by fabricating the edges of this two-dimensional material. Electron lone pairs (ELPs) are ubiquitous in black-P, but their role in creating the edge effects of black-P is poorly understood. Using first-principle calculations, we report ELPs of black-P experience severe Coulomb repulsion and play a central role in creating the edge effects of black-P. We discover the outermost P atoms of the zigzag edges of black-PQDs are free of the Coulomb repulsion, but the P atoms of the armchair edges do experience the Coulomb repulsion. The Coulomb repulsion serves as a new chemical driving force to make electron donor-acceptor bonds with chemical groups bearing vacant orbitals. Our results provide insights into the mechanism responsible for the peculiar edge effects of black-P and highlight the opportunity to use the ELPs of black-P for their damage-free surface functionalization.

Pyrolytic carbon coated black silicon

PubMed Central

Shah, Ali; Stenberg, Petri; Karvonen, Lasse; Ali, Rizwan; Honkanen, Seppo; Lipsanen, Harri; Peyghambarian, N.; Kuittinen, Markku; Svirko, Yuri; Kaplas, Tommi

2016-01-01

Carbon is the most well-known black material in the history of man. Throughout the centuries, carbon has been used as a black material for paintings, camouflage, and optics. Although, the techniques to make other black surfaces have evolved and become more sophisticated with time, carbon still remains one of the best black materials. Another well-known black surface is black silicon, reflecting less than 0.5% of incident light in visible spectral range but becomes a highly reflecting surface in wavelengths above 1000 nm. On the other hand, carbon absorbs at those and longer wavelengths. Thus, it is possible to combine black silicon with carbon to create an artificial material with very low reflectivity over a wide spectral range. Here we report our results on coating conformally black silicon substrate with amorphous pyrolytic carbon. We present a superior black surface with reflectance of light less than 0.5% in the spectral range of 350 nm to 2000 nm. PMID:27174890

The Attitudes of Blacks Toward Stories in Selected Basal Readers Which Contain Negro Characters: Teaching Black Experience Through Literature.

ERIC Educational Resources Information Center

Dantzler, Dolores J.

The purposes of the study were to determine the extent to which basal readers contain stories pertaining to blacks; to find out if the racial implications stated in basal texts are those that blacks want; to locate the black heroes in basal readers and evaluate whether or not these are the preferred heroes of the population of blacks sampled in…

The Effect of Motion Pictures Portraying Black Models on the Self-Concept of Black Elementary Students.

ERIC Educational Resources Information Center

Dimas, Chris

The purpose of this study was to determine the effects of motion pictures utilizing black models on the self-concept of black fourth and sixth grade students; that perhaps, mediated black models may be effective in bringing about a more positive self-concept among black students. The design of this study was of the post-test form only. This was…

40 CFR 458.10 - Applicability; description of the carbon black furnace process subcategory.

Code of Federal Regulations, 2010 CFR

2010-07-01

... carbon black furnace process subcategory. 458.10 Section 458.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Furnace Process Subcategory § 458.10 Applicability; description of the carbon black...

40 CFR 458.20 - Applicability: description of the carbon black thermal process subcategory.

Code of Federal Regulations, 2010 CFR

2010-07-01

... carbon black thermal process subcategory. 458.20 Section 458.20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Thermal Process Subcategory § 458.20 Applicability: description of the carbon black...

40 CFR 458.30 - Applicability; description of the carbon black channel process subcategory.

Code of Federal Regulations, 2010 CFR

2010-07-01

... carbon black channel process subcategory. 458.30 Section 458.30 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Channel Process Subcategory § 458.30 Applicability; description of the carbon black...

EVALUATION OF CARBON BLACK SLURRIES AS CLEAN BURNING FUELS

EPA Science Inventory

Experiments were performed to examine the pumpability, atomization and combustion characteristics of slurries made of mixtures of carbon black with No. 2 fuel oil and methanol. Carbon black-No. 2 fuel oil and carbon black-methanol slurries, with carbon black contents of up to 50 ...

77 FR 77070 - Black Bear Hydro Partners, LLC;

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-31

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 2727-086] Black Bear Hydro...: October 24, 2012. d. Submitted By: Black Bear Hydro Partners, LLC (Black Bear Hydro). e. Name of Project... designating Black Bear Hydro as the Commission's non-federal representative for carrying out informal...

Black Managers in White Corporations.

ERIC Educational Resources Information Center

Fernandez, John P.

The study examines the major determinants of the career patterns of black managers in white businesses and the effects of corporations on their black managers' identities and relationships to the black community. Analyzed were occupational mobility theories; white and black managers' career patterns, goals, and related factors; company employment…

News and Views.

ERIC Educational Resources Information Center

Journal of Blacks in Higher Education, 1999

1999-01-01

Presents a collection of articles that offer information on such issues as the progress of black student matriculations at the highest-ranking U.S. colleges; black historians teaching black history; Blacks in academic accounting; banishing the stereotype that African Americans cannot do mathematics; campus crime rates at black colleges; and the…

The Making of the Black Middle Class.

ERIC Educational Resources Information Center

Collins, Sharon M.

1983-01-01

Examines Black occupational mobility and factors that have influenced the growth of the Black middle class since the 1960s. Argues that the Black middle class occupies a fragile market position because Black mobility depends on fluctuating government policy rather than on free market factors. (Author/MJL)

Black Popular Music: First Scorned, Then Copied and Admired.

ERIC Educational Resources Information Center

Jones, Regina; Spiegelman, Judy

1991-01-01

Black popular music has its roots in African music that was a way of preserving history. A brief social and cultural history traces black popular music in the United States, focusing on the music blacks listened to, rather than just the music blacks played. (SLD)

Understanding the Black Aesthetic Experience.

ERIC Educational Resources Information Center

Curtis, Marvin V.

1988-01-01

Discussing the importance of the Black aesthetic experience, Curtis examines Black cultural heritage and participatory style, the spiritual, and the creation and recreation of Black music. Advocating multicultural music education in teacher training, he suggests that Black music be studied for its value and contribution to society. Lists five ways…

Personality Characteristics of Black Youth: A Cross-Cultural Investigation.

ERIC Educational Resources Information Center

Jones, Enrico E.

1979-01-01

Black adolescent girls of low socioeconomic status described themselves more favorably on the Adjective Check List than did Black and White middle-class adolescents. American and Jamaican Blacks described themselves similarly and differed similarly from White American conterparts, supporting the influence of a common cultural heritage for Blacks.…

Building Better Narratives in Black Education

ERIC Educational Resources Information Center

Anderson, Meredith B. L.

2016-01-01

"Building Better Narratives in Black Education" fundamentally changes the narrative and face of education reform to meaningfully include Black voices, leaders and initiatives that truly have equity and Black student success at the core. This is imperative as there is an education crisis for Black students in the United States. Recent…

New Directions for Black Women?

ERIC Educational Resources Information Center

McGuigan, Dorothy, Ed.

1978-01-01

This collection of papers deals with various aspects of the black female experience in America. "The Black Woman in Transition" discusses the effects of sexism and racism on black women with particular reference to employment and education; it is noted that black women, in comparison with other groups in society, suffer a proportionately higher…

"Nothing Has Stopped Me. I Keep Going:" Black Gay Narratives

ERIC Educational Resources Information Center

Bartone, Michael D.

2017-01-01

Are being Black and gay problematic in the United States? Are Black gay youth "suffering" with these two intersecting identities? Traditional Eurocentric epistemologies would have one believe that Black males, Black gay males, and gay males (liminal groups) are suffering, rarely acknowledging those successfully navigating through…

50 CFR 648.142 - Black sea bass specifications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass specifications. 648.142... Measures for the Black Sea Bass Fishery § 648.142 Black sea bass specifications. (a) Commercial quota, recreational landing limit, research set-aside, and other specification measures. The Black Sea Bass Monitoring...

50 CFR 648.142 - Black sea bass specifications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass specifications. 648.142... Measures for the Black Sea Bass Fishery § 648.142 Black sea bass specifications. (a) Commercial quota, recreational landing limit, research set-aside, and other specification measures. The Black Sea Bass Monitoring...

50 CFR 648.142 - Black sea bass specifications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Black sea bass specifications. 648.142... Measures for the Black Sea Bass Fishery § 648.142 Black sea bass specifications. (a) Commercial quota, recreational landing limit, research set-aside, and other specification measures. The Black Sea Bass Monitoring...

Black on Yellow: Afro-Americans View Chinese-Americans, 1850-1935

ERIC Educational Resources Information Center

Shankman, Arnold

1978-01-01

Using data collected from black newspapers, an explanation is sought for the unfavorable stereotypes blacks have held about Chinese Americans. Economic competition, cultural and religious differences, and blacks' belief that the Chinese were prejudiced against them are some of the historical factors mentioned which influenced black attitudes…

Census Undercount and the Undercount of the Black Population.

ERIC Educational Resources Information Center

Nguyen, Phung

1996-01-01

Argues that blacks have been disproportionately undercounted in the census and this undercount has resulted in such biased statistics as proportions of black female-headed households, victimization rates of black males, or crude death rates of blacks. Further, these statistics are biased upward and downward, and together they generate distorted…

Graduating Black Males

ERIC Educational Resources Information Center

Bell, Edward Earl

2010-01-01

Background: The graduation numbers for Black males are dismal, chilling, and undeniably pathetic. The nation graduates only 47% of Black males who enter the 9th grade. The infusion of federal dollars and philanthropic support will not stop the trajectory of Black males who drop out of school. Black males face an upheaval educational battle;…

78 FR 70297 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-25

.... Applicants: Black Bear Hydro Partners, LLC, Black Bear Development Holdings, LLC, Black Bear SO, LLC, Black Bear Holding LLC. Description: Joint Application for Authorization Under Section 203 of Black Bear...: 20131114-5164. Comments Due: 5 p.m. ET 12/5/13. Docket Numbers: ER14-407-000. Applicants: Capacity Markets...

Black Culture

ERIC Educational Resources Information Center

Brown, Angela Khristin

2013-01-01

The migration of blacks in North America through slavery became united. The population of blacks passed down a tradition of artist through art to native born citizens. The art tradition involved telling stories to each generation in black families. The black culture elevated by tradition created hope to determine their personal freedom to escape…

Meditation on Tuskegee: Black Studies Stories and Their Imbrication.

ERIC Educational Resources Information Center

Baker, Houston A., Jr.

1995-01-01

Discusses today's black studies programs and the division between the empiricist and conceptualist views of black history. Through a personal narrative involving a visit to Tuskegee University, the author muses over Booker T. Washington's type of eloquence in addressing the black experience compared to today's black public intellectual's attempts,…

Institutionalized Racism and the Education of Blacks

ERIC Educational Resources Information Center

Spears, Arthur K.

1978-01-01

Research into the causes of the unsatisfactory educational performance of many black students has focused on linguistic and cultural differences of blacks. Institutionalized racism, however, should be recognized as the major factor affecting black academic achievement. Black students should not be evaluated by the same criteria as white students.…

eBlack Studies: A Twenty-First-Century Challenge.

ERIC Educational Resources Information Center

Alkalimat, Abdul

2000-01-01

Calls for the transformation of black studies that moves from ideology to information. Argues that eBlack, the virtualization of the black experience, is the basis for the next stage of this academic discipline. Presents three theoretical principles of eBlack studies (cyberdemocracy, collective intelligence, and information freedom); describes…

Soul Music: Techniques for Therapeutic Intervention

ERIC Educational Resources Information Center

Toldson, Ivory L.; Pasteur, Alfred B.

1972-01-01

The authors conclude that using black music in the educational process aligns educational input with black culture. This approach, which extends and utilizes the spiritual dimension of black life, is consistent with the black value system and can help black learners integrate and internalize an aspect of their cultural heritage. (Author/BY)

Black Entrepreneurship in America.

ERIC Educational Resources Information Center

Green, Shelley; Pryde, Paul

The economic condition of black Americans is discussed, proceeding from the assumption that black economic progress does not depend on a renewed struggle for unobtained civil rights, but rather on the creative response of black Americans to economic opportunity and problems. In the long run, black economic development must rely on the…

The State of Black America 1987.

ERIC Educational Resources Information Center

Dewart, Janet, Ed.

This book consists of 12 papers on current issues affecting black Americans. Titles (and authors) are the following: (1) "Black America 1986: An Overview" (John E. Jacob); (2) "The Law and Black Americans: Retreat from Civil Rights" (Julius L. Chambers); (3) "Taking Charge: An Approach to Making the Educational Problems of Blacks Comprehensible…

Martin Robison Delany (1812-1885): physician, black separatist, explorer, soldier.

PubMed

Rosenfeld, L

1989-09-01

Martin Robison Delany's great contribution to American life and black history stems from his defiant blackness. He was America's first "Black Nationalist" and the intense embodiment of black pride. Ever changing in his career, he always identified with the black experience and its place in history. In an active life he was doctor, dentist, orator, editor, publisher, Harvard medical student, explorer, dabbler in Central American politics, army officer, and Reconstruction office seeker.

The Black Experience: Social, Cultural and Economic Considerations. Proceedings of a Workshop on the Black Experience. (1st, Chapel Hill, North Carolina, March 14, 1980).

ERIC Educational Resources Information Center

Johnson, Audreye E., Ed.

This publication consists of the proceedings of a workshop on the social, cultural, and economic experiences of Blacks. The workshops' goals were to intensify the interest of social workers in the Black experience; to examine the values which have an impact on services to Black people; to increase the knowledge of social workers about Blacks; and…

Accretion onto some well-known regular black holes

NASA Astrophysics Data System (ADS)

Jawad, Abdul; Shahzad, M. Umair

2016-03-01

In this work, we discuss the accretion onto static spherically symmetric regular black holes for specific choices of the equation of state parameter. The underlying regular black holes are charged regular black holes using the Fermi-Dirac distribution, logistic distribution, nonlinear electrodynamics, respectively, and Kehagias-Sftesos asymptotically flat regular black holes. We obtain the critical radius, critical speed, and squared sound speed during the accretion process near the regular black holes. We also study the behavior of radial velocity, energy density, and the rate of change of the mass for each of the regular black holes.

Enhanced performance of VOx-based bolometer using patterned gold black absorber

NASA Astrophysics Data System (ADS)

Smith, Evan M.; Panjwani, Deep; Ginn, James; Warren, Andrew; Long, Christopher; Figuieredo, Pedro; Smith, Christian; Perlstein, Joshua; Walter, Nick; Hirschmugl, Carol; Peale, Robert E.; Shelton, David J.

2015-06-01

Patterned highly absorbing gold black film has been selectively deposited on the active surfaces of a vanadium-oxide-based infrared bolometer array. Patterning by metal lift-off relies on protection of the fragile gold black with an evaporated oxide, which preserves gold black's near unity absorption. This patterned gold black also survives the dry-etch removal of the sacrificial polyimide used to fabricate the air-bridge bolometers. Infrared responsivity is substantially improved by the gold black coating without significantly increasing noise. The increase in the time constant caused by the additional mass of gold black is a modest 14%.

The weapon focus effect is weaker with Black versus White male perpetrators.

PubMed

Pickel, Kerri L; Sneyd, Danielle E

2018-01-01

We compared the influence of a weapon's presence on eyewitnesses' memory for a White versus a Black male perpetrator. Prior data indicate that unusual objects in visual scenes attract attention and that a weapon's effect depends on how unusual it seems within the context in which it appears. Therefore, given the stereotype linking Black men and weapons, we predicted a weaker weapon focus effect with the Black perpetrator. The results of Experiment 1 supported this hypothesis using White and Black witnesses. Moreover, in Experiment 2 the weapon focus effect became nonsignificant when the Black perpetrator wore a style of clothing that is strongly associated with Black men. We propose that observing an armed Black perpetrator automatically activates a stereotype linking Black men with weapons and crime, which in turn reduces the perceived unusualness of the weapon and thus its ability to attract attention.

The fragmentation instability of a black hole with f( R) global monopole under GUP

NASA Astrophysics Data System (ADS)

Chen, Lingshen; Cheng, Hongbo

2018-03-01

Having studied the fragmentation of the black holes containing f( R) global monopole under the generalized uncertainty principle (GUP), we show the influences from this kind of monopole, f( R) theory, and GUP on the evolution of black holes. We focus on the possibility that the black hole breaks into two parts by means of the second law of thermodynamics. We derive the entropies of the initial black hole and the broken parts while the generalization of Heisenberg's uncertainty principle is introduced. We find that the f( R) global monopole black hole keeps stable instead of splitting without the generalization because the entropy difference is negative. The fragmentation of the black hole will happen if the black hole entropies are limited by the GUP and the considerable deviation from the general relativity leads to the case that the mass of one fragmented black hole is smaller and the other one's mass is larger.

Novel platinum black electroplating technique improving mechanical stability.

PubMed

Kim, Raeyoung; Nam, Yoonkey

2013-01-01

Platinum black microelectrodes are widely used as an effective neural signal recording sensor. The simple fabrication process, high quality signal recording and proper biocompatibility are the main advantages of platinum black microelectrodes. When microelectrodes are exposed to actual biological system, various physical stimuli are applied. However, the porous structure of platinum black is vulnerable to external stimuli and destroyed easily. The impedance level of the microelectrode increases when the microelectrodes are damaged resulting in decreased recording performance. In this study, we developed mechanically stable platinum black microelectrodes by adding polydopamine. The polydopamine layer was added between the platinum black structures by electrodeposition method. The initial impedance level of platinum black only microelectrodes and polydopamine added microelectrodes were similar but after applying ultrasonication the impedance value dramatically increased for platinum black only microelectrodes, whereas polydopamine added microelectrodes showed little increase which were nearly retained initial values. Polydopamine added platinum black microelectrodes are expected to extend the availability as neural sensors.

Soft hair of dynamical black hole and Hawking radiation

NASA Astrophysics Data System (ADS)

Chu, Chong-Sun; Koyama, Yoji

2018-04-01

Soft hair of black hole has been proposed recently to play an important role in the resolution of the black hole information paradox. Recent work has emphasized that the soft modes cannot affect the black hole S-matrix due to Weinberg soft theorems. However as soft hair is generated by supertranslation of geometry which involves an angular dependent shift of time, it must have non-trivial quantum effects. We consider supertranslation of the Vaidya black hole and construct a non-spherical symmetric dynamical spacetime with soft hair. We show that this spacetime admits a trapping horizon and is a dynamical black hole. We find that Hawking radiation is emitted from the trapping horizon of the dynamical black hole. The Hawking radiation has a spectrum which depends on the soft hair of the black hole and this is consistent with the factorization property of the black hole S-matrix.

Entropy of black holes with multiple horizons

NASA Astrophysics Data System (ADS)

He, Yun; Ma, Meng-Sen; Zhao, Ren

2018-05-01

We examine the entropy of black holes in de Sitter space and black holes surrounded by quintessence. These black holes have multiple horizons, including at least the black hole event horizon and a horizon outside it (cosmological horizon for de Sitter black holes and "quintessence horizon" for the black holes surrounded by quintessence). Based on the consideration that the two horizons are not independent each other, we conjecture that the total entropy of these black holes should not be simply the sum of entropies of the two horizons, but should have an extra term coming from the correlations between the two horizons. Different from our previous works, in this paper we consider the cosmological constant as the variable and employ an effective method to derive the explicit form of the entropy. We also try to discuss the thermodynamic stabilities of these black holes according to the entropy and the effective temperature.

BlackMax: A black-hole event generator with rotation, recoil, split branes, and brane tension

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dai Dechang; Starkman, Glenn; Stojkovic, Dejan

2008-04-01

We present a comprehensive black-hole event generator, BlackMax, which simulates the experimental signatures of microscopic and Planckian black-hole production and evolution at the LHC in the context of brane world models with low-scale quantum gravity. The generator is based on phenomenologically realistic models free of serious problems that plague low-scale gravity, thus offering more realistic predictions for hadron-hadron colliders. The generator includes all of the black-hole gray-body factors known to date and incorporates the effects of black-hole rotation, splitting between the fermions, nonzero brane tension, and black-hole recoil due to Hawking radiation (although not all simultaneously). The generator can bemore » interfaced with Herwig and Pythia. The main code can be downloaded from http://www-pnp.physics.ox.ac.uk/{approx}issever/BlackMax/blackmax.html.« less

Black hole and cosmos with multiple horizons and multiple singularities in vector-tensor theories

NASA Astrophysics Data System (ADS)

Gao, Changjun; Lu, Youjun; Yu, Shuang; Shen, You-Gen

2018-05-01

A stationary and spherically symmetric black hole (e.g., Reissner-Nordström black hole or Kerr-Newman black hole) has, at most, one singularity and two horizons. One horizon is the outer event horizon and the other is the inner Cauchy horizon. Can we construct static and spherically symmetric black hole solutions with N horizons and M singularities? The de Sitter cosmos has only one apparent horizon. Can we construct cosmos solutions with N horizons? In this article, we present the static and spherically symmetric black hole and cosmos solutions with N horizons and M singularities in the vector-tensor theories. Following these motivations, we also construct the black hole solutions with a firewall. The deviation of these black hole solutions from the usual ones can be potentially tested by future measurements of gravitational waves or the black hole continuum spectrum.

Evolution of perturbations of squashed Kaluza-Klein black holes: Escape from instability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ishihara, Hideki; Kimura, Masashi; Konoplya, Roman A.

2008-04-15

The squashed Kaluza-Klien (KK) black holes differ from the Schwarzschild black holes with asymptotic flatness or the black strings even at energies for which the KK modes are not excited yet, so that squashed KK black holes open a window in higher dimensions. Another important feature is that the squashed KK black holes are apparently stable and, thereby, let us avoid the Gregory-Laflamme instability. In the present paper, the evolution of scalar and gravitational perturbations in time and frequency domains is considered for these squashed KK black holes. The scalar field perturbations are analyzed for general rotating squashed KK blackmore » holes. Gravitational perturbations for the so-called zero mode are shown to be decayed for nonrotating black holes, in concordance with the stability of the squashed KK black holes. The correlation of quasinormal frequencies with the size of extra dimension is discussed.« less

Thermodynamic studies of different black holes with modifications of entropy

NASA Astrophysics Data System (ADS)

Haldar, Amritendu; Biswas, Ritabrata

2018-02-01

In recent years, the thermodynamic properties of black holes are topics of interests. We investigate the thermodynamic properties like surface gravity and Hawking temperature on event horizon of regular black holes viz. Hayward Class and asymptotically AdS (Anti-de Sitter) black holes. We also analyze the thermodynamic volume and naive geometric volume of asymptotically AdS black holes and show that the entropy of these black holes is simply the ratio of the naive geometric volume to thermodynamic volume. We plot the different graphs and interpret them physically. We derive the `cosmic-Censorship-Inequality' for both type of black holes. Moreover, we calculate the thermal heat capacity of aforesaid black holes and study their stabilities in different regimes. Finally, we compute the logarithmic correction to the entropy for both the black holes considering the quantum fluctuations around the thermal equilibrium and study the corresponding thermodynamics.

The use of trivalent chromium bath to obtain a solar selective black chromium coating

NASA Astrophysics Data System (ADS)

Survilienė, S.; Češūnienė, A.; Juškėnas, R.; Selskienė, A.; Bučinskienė, D.; Kalinauskas, P.; Juškevičius, K.; Jurevičiūtė, I.

2014-06-01

Black chromium coatings were electrodeposited from a trivalent chromium bath using a ZnO additive as a second main component. Black chromium was electrodeposited on steel and copper plates and substrates plated with bright nickel prior to black chromium electrodeposition. The black chromium coatings were characterized by XRD and SEM. The XRD data suggest that the phase structure of black chromium may be defined as a zinc solid solution in chromium or a chromium solid solution in zinc depending on the chromium/zinc ratio in the deposit. The role of substrate finish was evaluated through the corrosion resistance and reflectance of black chromium. According to corrosion tests the samples plated with bright nickel prior to black chromium deposition have shown the highest corrosion resistance. The electrodeposited black chromium possesses good optical properties for the absorption of solar energy. The absorption coefficient of black chromium was found to be over 0.99 for the samples obtained without the Ni undercoat and below 0.99 for those obtained with the use of Ni undercoat. However, the use of nickel undercoat before black chromium plating is recommended because it remarkably improves the corrosion resistance of samples.

Gravitational tension, spacetime pressure and black hole volume

NASA Astrophysics Data System (ADS)

Armas, Jay; Obers, Niels A.; Sanchioni, Marco

2016-09-01

We study the first law of black hole thermodynamics in the presence of surrounding gravitational fields and argue that variations of these fields are naturally incorporated in the first law by defining gravitational tension or gravitational binding energy. We demonstrate that this notion can also be applied in Anti-de Sitter spacetime, in which the surrounding gravitational field is sourced by a cosmological fluid, therefore showing that spacetime volume and gravitational tension encode the same physics as spacetime pressure and black hole volume. We furthermore show that it is possible to introduce a definition of spacetime pressure and black hole volume for any spacetime with characteristic length scales which does not necessarily require a cosmological constant sourcing Einstein equations. However, we show that black hole volume is non-universal in the flat spacetime limit, questioning its significance. We illustrate these ideas by studying the resulting black hole volume of Kaluza-Klein black holes and of a toy model for a black hole binary system in five spacetime dimensions (the black saturn solution) as well as of several novel perturbative black hole solutions. These include the higher-dimensional Kerr-Newman solution in Anti-de Sitter spacetime as well as other black holes in plane wave and Lifshitz spacetimes.

Entropy is conserved in Hawking radiation as tunneling: A revisit of the black hole information loss paradox

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang Baocheng; Graduate University of Chinese Academy of Sciences, Beijing 100049; Cai Qingyu, E-mail: qycai@wipm.ac.cn

2011-02-15

Research Highlights: > Information is found to be encoded and carried away by Hawking radiations. > Entropy is conserved in Hawking radiation. > We thus conclude no information is lost. > The dynamics of black hole may be unitary. - Abstract: We revisit in detail the paradox of black hole information loss due to Hawking radiation as tunneling. We compute the amount of information encoded in correlations among Hawking radiations for a variety of black holes, including the Schwarzchild black hole, the Reissner-Nordstroem black hole, the Kerr black hole, and the Kerr-Newman black hole. The special case of tunneling throughmore » a quantum horizon is also considered. Within a phenomenological treatment based on the accepted emission probability spectrum from a black hole, we find that information is leaked out hidden in the correlations of Hawking radiation. The recovery of this previously unaccounted for information helps to conserve the total entropy of a system composed of a black hole plus its radiations. We thus conclude, irrespective of the microscopic picture for black hole collapsing, the associated radiation process: Hawking radiation as tunneling, is consistent with unitarity as required by quantum mechanics.« less

Structure of Black Male Students Academic Achievement in Science

NASA Astrophysics Data System (ADS)

Rascoe, Barbara

Educational policies and practices have been largely unsuccessful in closing the achievement gap between Black and White students "Schwartz, 2001". This achievement gap is especially problematic for Black students in science "Maton, Hrabrowski, - Schmitt, 2000. Given the fact that the Black-White achievement gap is still an enigma, the purpose of this article is to address the Black female-Black male academic achievement gap in science majors. Addressing barriers that Black male students may experience as college science and engineering majors, this article presents marketing strategies relative to politics, emotional intelligence, and issues with respect to how science teaching, and Black male students' responses to it, are different. Many Black male students may need to experience a paradigm shift, which structures and enhances their science achievement. Paradigm shifts are necessary because exceptional academic ability and motivation are not enough to get Black males from their first year in a science, technology, education, and mathematics "STEM" major to a bachelor's degree in science and engineering. The conclusions focus on the balance of truth-slippery slopes concerning the confluence of science teachers' further ado and Black male students' theories, methods, and values that position their academic achievement in science and engineering majors.

Big Black Holes Mean Bad News for Stars (diagram)

NASA Technical Reports Server (NTRS)

2006-01-01

[figure removed for brevity, see original site] Poster Version Suppression of Star Formation from Supermassive Black Holes

This diagram illustrates research from NASA's Galaxy Evolution Explorer showing that black holes -- once they reach a critical size -- can put the brakes on new star formation in elliptical galaxies.

In this graph, galaxies and their supermassive black holes are indicated by the drawings (the black circle at the center of each galaxy represents the black hole). The relative masses of the galaxies and their black holes are reflected in the sizes of the drawings. Blue indicates that the galaxy has new stars, while red means the galaxy does not have any detectable new stars.

The Galaxy Evolution Explorer observed the following trend: the biggest galaxies and black holes (shown in upper right corner) are more likely to have no observable star formation (red) than the smaller galaxies with smaller black holes. This is evidence that black holes can create environments unsuitable for stellar birth.

The white line in the diagram illustrates that, for any galaxy no matter what the mass, its black hole must reach a critical size before it can shut down star formation.

Bird specimens track 135 years of atmospheric black carbon and environmental policy

NASA Astrophysics Data System (ADS)

DuBay, Shane G.; Fuldner, Carl C.

2017-10-01

Atmospheric black carbon has long been recognized as a public health and environmental concern. More recently, black carbon has been identified as a major, ongoing contributor to anthropogenic climate change, thus making historical emission inventories of black carbon an essential tool for assessing past climate sensitivity and modeling future climate scenarios. Current estimates of black carbon emissions for the early industrial era have high uncertainty, however, because direct environmental sampling is sparse before the mid-1950s. Using photometric reflectance data of >1,300 bird specimens drawn from natural history collections, we track relative ambient concentrations of atmospheric black carbon between 1880 and 2015 within the US Manufacturing Belt, a region historically reliant on coal and dense with industry. Our data show that black carbon levels within the region peaked during the first decade of the 20th century. Following this peak, black carbon levels were positively correlated with coal consumption through midcentury, after which they decoupled, with black carbon concentrations declining as consumption continued to rise. The precipitous drop in atmospheric black carbon at midcentury reflects policies promoting burning efficiency and fuel transitions rather than regulating emissions alone. Our findings suggest that current emission inventories based on predictive modeling underestimate levels of atmospheric black carbon for the early industrial era, suggesting that the contribution of black carbon to past climate forcing may also be underestimated. These findings build toward a spatially dynamic emission inventory of black carbon based on direct environmental sampling.

Risky movies, risky behaviors, and ethnic identity among Black adolescents.

PubMed

Bleakley, Amy; Ellithorpe, Morgan E; Hennessy, Michael; Jamieson, Patrick E; Khurana, Atika; Weitz, Ilana

2017-12-01

To investigate how exposure to sex, alcohol and violent content in mainstream and Black-oriented movies relates to corresponding adolescent behavior among Black youth from the United States and whether those relationships are moderated by ethnic identity. The present study uses survey data from an online sample of 1000 Black adolescents and content analysis ratings on top-grossing 2014 films and 2013/2014 Black-oriented films. Content-specific exposure measures for alcohol, sexual activity, and violence were calculated from self-reported exposure data and content analysis ratings. Regression analyses estimated the associations among exposures to risky health content in mainstream and Black-oriented films and adolescent behaviors as well as moderation by ethnic group identity. Black adolescents were mostly unaffected by exposure to risk portrayals in mainstream films, but exposure to risk in Black-oriented films was related to their behavior in all three domains. Strong group identity strengthened the relationship between exposure to sex in Black-oriented and mainstream films depending on the sexual outcome. The type of movie (i.e., mainstream or Black-oriented) through which Black adolescents are exposed to risky health portrayals is important for understanding its relationship to their behavior, and variations by ethnic identity were limited to sex content. Future research should identify the mechanisms through which risk content in Black-oriented films is associated with Black adolescents' risky behaviors to determine how media influence contributes to behavioral disparities among youth. Copyright © 2017 Elsevier Ltd. All rights reserved.

Bird specimens track 135 years of atmospheric black carbon and environmental policy

PubMed Central

DuBay, Shane G.; Fuldner, Carl C.

2017-01-01

Atmospheric black carbon has long been recognized as a public health and environmental concern. More recently, black carbon has been identified as a major, ongoing contributor to anthropogenic climate change, thus making historical emission inventories of black carbon an essential tool for assessing past climate sensitivity and modeling future climate scenarios. Current estimates of black carbon emissions for the early industrial era have high uncertainty, however, because direct environmental sampling is sparse before the mid-1950s. Using photometric reflectance data of >1,300 bird specimens drawn from natural history collections, we track relative ambient concentrations of atmospheric black carbon between 1880 and 2015 within the US Manufacturing Belt, a region historically reliant on coal and dense with industry. Our data show that black carbon levels within the region peaked during the first decade of the 20th century. Following this peak, black carbon levels were positively correlated with coal consumption through midcentury, after which they decoupled, with black carbon concentrations declining as consumption continued to rise. The precipitous drop in atmospheric black carbon at midcentury reflects policies promoting burning efficiency and fuel transitions rather than regulating emissions alone. Our findings suggest that current emission inventories based on predictive modeling underestimate levels of atmospheric black carbon for the early industrial era, suggesting that the contribution of black carbon to past climate forcing may also be underestimated. These findings build toward a spatially dynamic emission inventory of black carbon based on direct environmental sampling. PMID:29073051

Two stellar-mass black holes in the globular cluster M22.

PubMed

Strader, Jay; Chomiuk, Laura; Maccarone, Thomas J; Miller-Jones, James C A; Seth, Anil C

2012-10-04

Hundreds of stellar-mass black holes probably form in a typical globular star cluster, with all but one predicted to be ejected through dynamical interactions. Some observational support for this idea is provided by the lack of X-ray-emitting binary stars comprising one black hole and one other star ('black-hole/X-ray binaries') in Milky Way globular clusters, even though many neutron-star/X-ray binaries are known. Although a few black holes have been seen in globular clusters around other galaxies, the masses of these cannot be determined, and some may be intermediate-mass black holes that form through exotic mechanisms. Here we report the presence of two flat-spectrum radio sources in the Milky Way globular cluster M22, and we argue that these objects are black holes of stellar mass (each ∼10-20 times more massive than the Sun) that are accreting matter. We find a high ratio of radio-to-X-ray flux for these black holes, consistent with the larger predicted masses of black holes in globular clusters compared to those outside. The identification of two black holes in one cluster shows that ejection of black holes is not as efficient as predicted by most models, and we argue that M22 may contain a total population of ∼5-100 black holes. The large core radius of M22 could arise from heating produced by the black holes.

An intermediate-mass black hole in the centre of the globular cluster 47 Tucanae.

PubMed

Kızıltan, Bülent; Baumgardt, Holger; Loeb, Abraham

2017-02-08

Intermediate-mass black holes should help us to understand the evolutionary connection between stellar-mass and super-massive black holes. However, the existence of intermediate-mass black holes is still uncertain, and their formation process is therefore unknown. It has long been suspected that black holes with masses 100 to 10,000 times that of the Sun should form and reside in dense stellar systems. Therefore, dedicated observational campaigns have targeted globular clusters for many decades, searching for signatures of these elusive objects. All candidate signatures appear radio-dim and do not have the X-ray to radio flux ratios required for accreting black holes. Based on the lack of an electromagnetic counterpart, upper limits of 2,060 and 470 solar masses have been placed on the mass of a putative black hole in 47 Tucanae (NGC 104) from radio and X-ray observations, respectively. Here we show there is evidence for a central black hole in 47 Tucanae with a mass of solar masses when the dynamical state of the globular cluster is probed with pulsars. The existence of an intermediate-mass black hole in the centre of one of the densest clusters with no detectable electromagnetic counterpart suggests that the black hole is not accreting at a sufficient rate to make it electromagnetically bright and therefore, contrary to expectations, is gas-starved. This intermediate-mass black hole might be a member of an electromagnetically invisible population of black holes that grow into supermassive black holes in galaxies.

Barriers to early diagnosis of symptomatic breast cancer: a qualitative study of Black African, Black Caribbean and White British women living in the UK.

PubMed

Jones, Claire E L; Maben, Jill; Lucas, Grace; Davies, Elizabeth A; Jack, Ruth H; Ream, Emma

2015-03-13

Understanding barriers to early diagnosis of symptomatic breast cancer among Black African, Black Caribbean and White British women in the UK. In-depth qualitative interviews using grounded theory methods to identify themes. Findings validated through focus groups. 94 women aged 33-91 years; 20 Black African, 20 Black Caribbean and 20 White British women diagnosed with symptomatic breast cancer were interviewed. Fourteen Black African and 20 Black Caribbean women with (n=19) and without (n=15) breast cancer participated in six focus groups. Eight cancer centres/hospital trusts in London (n=5), Somerset (n=1), West Midlands (n=1) and Greater Manchester (n=1) during 2012-2013. There are important differences and similarities in barriers to early diagnosis of breast cancer between Black African, Black Caribbean and White British women in the UK. Differences were influenced by country of birth, time spent in UK and age. First generation Black African women experienced most barriers and longest delays. Second generation Black Caribbean and White British women were similar and experienced fewest barriers. Absence of pain was a barrier for Black African and Black Caribbean women. Older White British women (≥70 years) and first generation Black African and Black Caribbean women shared conservative attitudes and taboos about breast awareness. All women viewed themselves at low risk of the disease, and voiced uncertainty over breast awareness and appraising non-lump symptoms. Focus group findings validated and expanded themes identified in interviews. Findings challenged reporting of Black women homogenously in breast cancer research. This can mask distinctions within and between ethnic groups. Current media and health promotion messages need reframing to promote early presentation with breast symptoms. Working with communities and developing culturally appropriate materials may lessen taboos and stigma, raise awareness, increase discussion of breast cancer and promote prompt help-seeking for breast symptoms among women with low cancer awareness. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Barriers to early diagnosis of symptomatic breast cancer: a qualitative study of Black African, Black Caribbean and White British women living in the UK

PubMed Central

Jones, Claire E L; Maben, Jill; Lucas, Grace; Davies, Elizabeth A; Jack, Ruth H; Ream, Emma

2015-01-01

Objectives Understanding barriers to early diagnosis of symptomatic breast cancer among Black African, Black Caribbean and White British women in the UK. Design In-depth qualitative interviews using grounded theory methods to identify themes. Findings validated through focus groups. Participants 94 women aged 33–91 years; 20 Black African, 20 Black Caribbean and 20 White British women diagnosed with symptomatic breast cancer were interviewed. Fourteen Black African and 20 Black Caribbean women with (n=19) and without (n=15) breast cancer participated in six focus groups. Setting Eight cancer centres/hospital trusts in London (n=5), Somerset (n=1), West Midlands (n=1) and Greater Manchester (n=1) during 2012–2013. Results There are important differences and similarities in barriers to early diagnosis of breast cancer between Black African, Black Caribbean and White British women in the UK. Differences were influenced by country of birth, time spent in UK and age. First generation Black African women experienced most barriers and longest delays. Second generation Black Caribbean and White British women were similar and experienced fewest barriers. Absence of pain was a barrier for Black African and Black Caribbean women. Older White British women (≥70 years) and first generation Black African and Black Caribbean women shared conservative attitudes and taboos about breast awareness. All women viewed themselves at low risk of the disease, and voiced uncertainty over breast awareness and appraising non-lump symptoms. Focus group findings validated and expanded themes identified in interviews. Conclusions Findings challenged reporting of Black women homogenously in breast cancer research. This can mask distinctions within and between ethnic groups. Current media and health promotion messages need reframing to promote early presentation with breast symptoms. Working with communities and developing culturally appropriate materials may lessen taboos and stigma, raise awareness, increase discussion of breast cancer and promote prompt help-seeking for breast symptoms among women with low cancer awareness. PMID:25770231

NASA Observatory Confirms Black Hole Limits

NASA Astrophysics Data System (ADS)

2005-02-01

The very largest black holes reach a certain point and then grow no more, according to the best survey to date of black holes made with NASA's Chandra X-ray Observatory. Scientists have also discovered many previously hidden black holes that are well below their weight limit. These new results corroborate recent theoretical work about how black holes and galaxies grow. The biggest black holes, those with at least 100 million times the mass of the Sun, ate voraciously during the early Universe. Nearly all of them ran out of 'food' billions of years ago and went onto a forced starvation diet. Focus on Black Holes in the Chandra Deep Field North Focus on Black Holes in the Chandra Deep Field North On the other hand, black holes between about 10 and 100 million solar masses followed a more controlled eating plan. Because they took smaller portions of their meals of gas and dust, they continue growing today. "Our data show that some supermassive black holes seem to binge, while others prefer to graze", said Amy Barger of the University of Wisconsin in Madison and the University of Hawaii, lead author of the paper describing the results in the latest issue of The Astronomical Journal (Feb 2005). "We now understand better than ever before how supermassive black holes grow." One revelation is that there is a strong connection between the growth of black holes and the birth of stars. Previously, astronomers had done careful studies of the birthrate of stars in galaxies, but didn't know as much about the black holes at their centers. DSS Optical Image of Lockman Hole DSS Optical Image of Lockman Hole "These galaxies lose material into their central black holes at the same time that they make their stars," said Barger. "So whatever mechanism governs star formation in galaxies also governs black hole growth." Astronomers have made an accurate census of both the biggest, active black holes in the distance, and the relatively smaller, calmer ones closer by. Now, for the first time, the ones in between have been counted properly. Growth of the Biggest Black Holes Illustrated Growth of the Biggest Black Holes Illustrated "We need to have an accurate head count over time of all growing black holes if we ever hope to understand their habits, so to speak," co-author Richard Mushotzky of NASA's Goddard Space Flight Center in Greenbelt, Md. Supermassive black holes themselves are invisible, but heated gas around them -- some of which will eventually fall into the black hole - produces copious amounts of radiation in the centers of galaxies as the black holes grow. Growth of the Biggest Black Holes Illustrated Growth of Smaller Black Holes Illustrated This study relied on the deepest X-ray images ever obtained, the Chandra Deep Fields North and South, plus a key wider-area survey of an area called the "Lockman Hole". The distances to the X-ray sources were determined by optical spectroscopic follow-up at the Keck 10-meter telescope on Mauna Kea in Hawaii, and show the black holes range from less than a billion to 12 billion light years away. Since X-rays can penetrate the gas and dust that block optical and ultraviolet emission, the very long-exposure X-ray images are crucial to find black holes that otherwise would go unnoticed. Black Hole Animation Black Hole Animation Chandra found that many of the black holes smaller than about 100 million Suns are buried under large amounts of dust and gas, which prevents detection of the optical light from the heated material near the black hole. The X-rays are more energetic and are able to burrow through this dust and gas. However, the largest of the black holes show little sign of obscuration by dust or gas. In a form of weight self-control, powerful winds generated by the black hole's feeding frenzy may have cleared out the remaining dust and gas. Other aspects of black hole growth were uncovered. For example, the typical size of the galaxies undergoing supermassive black hole formation reduces with cosmic time. Such "cosmic downsizing" was previously observed for galaxies undergoing star formation. These results connect well with the observations of nearby galaxies, which find that the mass of a supermassive black hole is proportional to the mass of the central region of its host galaxy. The other co-authors on the paper in the February 2005 issue of The Astronomical Journal were Len Cowie, Wei-Hao Wang, and Peter Capak (Institute for Astronomy, Univ. of Hawaii), Yuxuan Yang (GSFC and the Univ. of Maryland, College Park), and Aaron Steffen (Univ. of Wisconsin, Madison). NASA's Marshall Space Flight Center, Huntsville, Ala., manages the Chandra program for NASA's Space Mission Directorate, Washington. Northrop Grumman of Redondo Beach, Calif., formerly TRW, Inc., was the prime development contractor for the observatory. The Smithsonian Astrophysical Observatory controls science and flight operations from the Chandra X-ray Center in Cambridge, Mass. Additional information and images are available at: http://chandra.harvard.edu and http://chandra.nasa.gov

Bioavailability of catechins from tea: the effect of milk.

PubMed

van het Hof, K H; Kivits, G A; Weststrate, J A; Tijburg, L B

1998-05-01

To assess the blood concentration of catechins following green or black tea ingestion and the effect of addition of milk to black tea. Twelve volunteers received a single dose of green tea, black tea and black tea with milk in a randomized cross-over design with one-week intervals. Blood samples were drawn before and up to eight hours after tea consumption. The study was performed at the Unilever Research Vlaardingen in The Netherlands. Twelve healthy adult volunteers (7 females, 5 males) participated in the study. They were recruited among employees of Unilever Research Vlaardingen. Green tea, black tea and black tea with semi-skimmed milk (3 g tea solids each). Consumption of green tea (0.9 g total catechins) or black tea (0.3 g total catechins) resulted in a rapid increase of catechin levels in blood with an average maximum change from baseline (CVM) of 0.46 micromol/l (13%) after ingestion of green tea and 0.10 micromol/l (13%) in case of black tea. These maximum changes were reached after (mean (s.e.m.)) t=2.3 h (0.2) and t=2.2 h (0.2) for green and black tea respectively. Blood levels rapidly declined with an elimination rate (mean (CVM)) of t1/2=4.8 h (5%) for green tea and t1/2=6.9 h (8%) for black tea. Addition of milk to black tea (100 ml in 600 ml) did not significantly affect the blood catechin levels (areas under the curves (mean (CVM) of 0.53 h. micromol/l (11%) vs 0.60 h. micromol/l (9%) for black tea and black tea with milk respectively. Catechins from green tea and black tea are rapidly absorbed and milk does not impair the bioavailability of tea catechins.

Thermodynamic properties of charged three-dimensional black holes in the scalar-tensor gravity theory

NASA Astrophysics Data System (ADS)

Dehghani, M.

2018-02-01

Making use of the suitable transformation relations, the action of three-dimensional Einstein-Maxwell-dilaton gravity theory has been obtained from that of scalar-tensor modified gravity theory coupled to the Maxwell's electrodynamics as the matter field. Two new classes of the static three-dimensional charged dilatonic black holes, as the exact solutions to the coupled scalar, electromagnetic and gravitational field equations, have been obtained in the Einstein frame. Also, it has been found that the scalar potential can be written in the form of a generalized Liouville-type potential. The conserved black hole charge and masses as well as the black entropy, temperature, and electric potential have been calculated from the geometrical and thermodynamical approaches, separately. Through comparison of the results arisen from these two alternative approaches, the validity of the thermodynamical first law has been proved for both of the new black hole solutions in the Einstein frame. Making use of the canonical ensemble method, a black hole stability or phase transition analysis has been performed. Regarding the black hole heat capacity, with the black hole charge as a constant, the points of type-1 and type-2 phase transitions have been determined. Also, the ranges of the black hole horizon radius at which the Einstein black holes are thermally stable have been obtained for both of the new black hole solutions. Then making use of the inverse transformation relations, two new classes of the string black hole solutions have been obtained from their Einstein counterpart. The thermodynamics and thermal stability of the new string black hole solutions have been investigated. It has been found that thermodynamic properties of the new charged black holes are identical in the Einstein and Jordan frames.

Skyrmion black hole hair: Conservation of baryon number by black holes and observable manifestations

NASA Astrophysics Data System (ADS)

Dvali, Gia; Gußmann, Alexander

2016-12-01

We show that the existence of black holes with classical skyrmion hair invalidates standard proofs that global charges, such as the baryon number, cannot be conserved by a black hole. By carefully analyzing the standard arguments based on a Gedankenexperiment in which a black hole is seemingly-unable to return the baryon number that it swallowed, we identify inconsistencies in this reasoning, which does not take into the account neither the existence of skyrmion black holes nor the baryon/skyrmion correspondence. We then perform a refined Gedankenexperiment by incorporating the new knowledge and show that no contradiction with conservation of baryon number takes place at any stage of black hole evolution. Our analysis also indicates no conflict between semi-classical black holes and the existence of baryonic gauge interaction arbitrarily-weaker than gravity. Next, we study classical cross sections of a minimally-coupled massless probe scalar field scattered by a skyrmion black hole. We investigate how the skyrmion hair manifests itself by comparing this cross section with the analogous cross section caused by a Schwarzschild black hole which has the same ADM mass as the skyrmion black hole. Here we find an order-one difference in the positions of the characteristic peaks in the cross sections. The peaks are shifted to smaller scattering angles when the skyrmion hair is present. This comes from the fact that the skyrmion hair changes the near horizon geometry of the black hole when compared to a Schwarzschild black hole with same ADM mass. We keep the study of this second aspect general so that the qualitative results which we obtain can also be applied to black holes with classical hair of different kind.

Algebraically special resonances of the Kerr-black-hole-mirror bomb

NASA Astrophysics Data System (ADS)

Hod, Shahar

2013-12-01

A corotating bosonic field interacting with a spinning Kerr black hole can extract rotational energy and angular momentum from the hole. This intriguing phenomenon is known as superradiant scattering. As pointed out by Press and Teukolsky, the black-hole-field system can be made unstable (explosive) by placing a reflecting mirror around the black hole, which prevents the extracted energy from escaping to infinity. This composed black-hole-mirror-field bomb has been studied extensively by many researchers. It is worth noting, however, that most former studies of the black-hole bomb phenomenon have focused on the specific case of confined scalar (spin-0) fields. In the present study we explore the physical properties of the higher-spin (electromagnetic and gravitational) black-hole bombs. It is shown that this composed system is amenable to an analytic treatment in the physically interesting regime of rapidly rotating black holes. In particular, we prove that the composed black-hole-mirror-field bomb is characterized by the unstable resonance frequency ω=mΩH+is·2πTBH (here s and m are, respectively, the spin parameter and the azimuthal harmonic index of the field, and ΩH and TBH are, respectively, the angular-velocity and the temperature of the rapidly spinning black hole). Our results provide evidence that the higher-spin (electromagnetic and gravitational) black-hole-mirror bombs are much more explosive than the extensively studied scalar black-hole-mirror bomb. In particular, it is shown here that the instability growth rates that characterize the higher-spin black-hole bombs are 2 orders of magnitude larger than the instability growth rate of the scalar black-hole bomb.

Sexual Stereotypes Ascribed to Black Men Who Have Sex with Men: An Intersectional Analysis.

PubMed

Calabrese, Sarah K; Earnshaw, Valerie A; Magnus, Manya; Hansen, Nathan B; Krakower, Douglas S; Underhill, Kristen; Mayer, Kenneth H; Kershaw, Trace S; Betancourt, Joseph R; Dovidio, John F

2018-01-01

Sexual stereotypes may adversely affect the health of Black men who have sex with men (MSM). Greater understanding of the nature and nuances of these stereotypes is needed. This online, survey-based study used an inductive, intersectional approach to characterize the sexual stereotypes ascribed to Black MSM by the U.S. general public, their distinctiveness from those ascribed to Black men and MSM in general, and their relative prototypicality as compared to dominant subgroups. Members of the public, recruited in 2014-2015, were randomly assigned to survey conditions that varied systematically by race (Black, White, or unspecified) and sexual orientation (gay, heterosexual, or unspecified) of a designated social group. Participants (n = 285) reported stereotypes of their assigned group that they perceived to exist in U.S. culture in an open-response format. Cross-condition comparisons revealed that, overall, Black gay male stereotypes were non-prototypical of Black men or gay men. Rather, stereotypes of Black men were more similar to Black heterosexual men and stereotypes of gay men were more similar to White gay men. Nonetheless, 11 of the 15 most frequently reported Black gay male stereotypes overlapped with stereotypes of Black men (e.g., large penis), gay men (e.g., deviant), or both (e.g., promiscuous). Four stereotypes were unique relative to both Black men and gay men: down low, diseased, loud, and dirty. Findings suggest that Black MSM face multiple derogatory sexual stereotypes, several of which are group-specific. These stereotypes are consistent with cultural (mis)representations of Black MSM and suggest a need for more accurate portrayals of existing sexual diversity within this group.

Exploration of Factors Affecting Success of Undergraduate Engineering Majors at a Historically Black University

NASA Astrophysics Data System (ADS)

Igbinoba, Egheosa P.

Blacks are underrepresented amongst persons who earn college degrees in the United States and Black males attend and complete college at a lower rate than Black females (Toldson, Fry Brown, & Sutton, 2009). According to Toldson et al. (2009), this quandary may be attributed to Black males' apathy toward education in general, waning support and ideological challenges toward Pell Grants and affirmative action, cultural incompetency on the part of the 90% White, ethnic makeup of the U.S. teaching force, and the relatively high numbers of Black males who are held back in school. In spite of the dismal statistics regarding Black male academic achievement and matriculation, there are those Black males who do participate in postsecondary education. While many studies have highlighted reasons that Black males do not achieve success in attending and persisting through college, few have adopted the anti-deficit research framework suggested by Harper (2010), identifying reasons Black males do persist in higher education. Although science, technology, engineering, and mathematics careers are identified as those most imperative to the economic competitiveness of the United States, few studies have concentrated solely on engineering majors and fewer, if any, solely on Black male engineering majors at an historically Black college and university. The aim of this study was to address an apparent gap in the literature and invoke theories for recruitment, retention, and success of Black males in engineering degree programs by employing an anti-deficit achievement framework for research of students of color in science, technology, engineering, and mathematics. Data garnered from the study included insight into participants' definitions of success, precollege experiences, factors contributing to the persistence during undergraduate study, and perceptions of attending a historically Black college and university versus a primarily White institution.

Effects of shading on growth and development of northern red oak, black oak, black cherry, and red maple seedlings. II. biomass partitioning and prediction

Treesearch

Kurt W. Gottschalk

1987-01-01

Northern red oak, black oak, black cherry, and red maple seedlings were grown under light treatments ranging from 8 to 94% of full sunlight for 2 years. Growth was least at the lowest light level and total dry weight increased with increasing light. Total dry-weight rankings (largest to smallest) at all light levels were black cherry, northern red oak, black oak, and...

Alcohol Use and Mental Health Conditions Among Black College Males: Do Those Attending Postsecondary Minority Institutions Fare Better Than Those at Primarily White Institutions?

PubMed

Barry, Adam E; Jackson, Zachary; Watkins, Daphne C; Goodwill, Janelle R; Hunte, Haslyn E R

2017-07-01

While there is a sizeable body of research examining the association between alcohol use and mental health conditions among college students, there are sparse investigations specifically focusing on these associations among Black college students. This is concerning given Black college students face different stressors compared with their non-Black peers. Black males appear especially at risk, exhibiting increased susceptibility to mental health issues and drinking in greater quantities and more frequently than Black females. This investigation examined the association between alcohol consumption and mental health conditions among Black men attending institutions of higher education in the United States and sought to determine differences between Black men attending predominantly White institutions (PWIs) compared with those attending postsecondary minority institutions. Final sample included 416 Black men, 323 of which attended a PWI. Data were from the National College Health Assessment. Black men attending a PWI reported significantly greater levels of alcohol consumption and significantly more mental health conditions. Attendance at a minority-serving institution was associated with fewer mental health conditions among Black men. Future studies should seek to replicate these findings and conduct culturally sensitive and gender-specific research examining why Black men at PWIs report greater alcohol consumption and more mental health conditions than their peers attending postsecondary minority institutions.

Alcohol Use and Mental Health Conditions Among Black College Males: Do Those Attending Postsecondary Minority Institutions Fare Better Than Those at Primarily White Institutions?

PubMed Central

Barry, Adam E.; Jackson, Zachary; Watkins, Daphne C.; Goodwill, Janelle R.; Hunte, Haslyn E.R.

2016-01-01

While there is a sizeable body of research examining the association between alcohol use and mental health conditions among college students, there are sparse investigations specifically focusing on these associations among Black college students. This is concerning given Black college students face different stressors compared with their non-Black peers. Black males appear especially at risk, exhibiting increased susceptibility to mental health issues and drinking in greater quantities and more frequently than Black females. This investigation examined the association between alcohol consumption and mental health conditions among Black men attending institutions of higher education in the United States and sought to determine differences between Black men attending predominantly White institutions (PWIs) compared with those attending postsecondary minority institutions. Final sample included 416 Black men, 323 of which attended a PWI. Data were from the National College Health Assessment. Black men attending a PWI reported significantly greater levels of alcohol consumption and significantly more mental health conditions. Attendance at a minority-serving institution was associated with fewer mental health conditions among Black men. Future studies should seek to replicate these findings and conduct culturally sensitive and gender-specific research examining why Black men at PWIs report greater alcohol consumption and more mental health conditions than their peers attending postsecondary minority institutions. PMID:27807223

Where to Focus Efforts to Reduce the Black-White Disparity In Stroke Mortality: Incidence vs. Case-Fatality?

PubMed Central

Howard, George; Moy, Claudia S.; Howard, Virginia J.; McClure, Leslie A.; Kleindorfer, Dawn O.; Kissela, Brett M.; Judd, Suzanne E.; Unverzagt, Fredrick W.; Soliman, Elsayed Z.; Safford, Monika M.; Cushman, Mary; Flaherty, Matthew L.; Wadley, Virginia G.

2016-01-01

Background and Purpose At age 45, Blacks have a stroke mortality approximately 3-times greater than their White counterparts, with a declining disparity at older ages. We assess whether this Black-White disparity in stroke mortality is attributable to a Black-White disparity in stroke incidence versus a disparity in case-fatality. Methods We first assess if Black-White differences in stroke mortality within 29,681 participants in the REasons for Geographic And Racial Differences in Stroke (REGARDS) cohort reflect national Black-White differences in stroke mortality, and then assess the degree to which Black-White differences in stroke incidence or 30-day case-fatality after stroke contribute to the disparities in stroke mortality. Results The pattern of stroke mortality within the study mirrors the national pattern, with the Black-to-White hazard ratio of approximately 4.0 at age 45 decreasing to approximately 1.0 at age 85. The pattern of Black-to-White disparities in stroke incidence shows a similar pattern, but no evidence of a corresponding disparity in stroke case-fatality. Discussion These findings show that the Black-White differences in stroke mortality are largely driven by differences in stroke incidence, with case fatality playing at most a minor role. Therefore to reduce the Black-White disparity in stroke mortality, interventions need to focus on prevention of stroke in Blacks. PMID:27256672

Performance of Black Grandmothers: Perceptions of Three Generations of Females

ERIC Educational Resources Information Center

Strom, Robert D.; Heeder, Steven D.; Strom, Paris S.

2005-01-01

The purposes of this study were to (a) identify perceptions of 3 generations about the influence of black grandmothers; (b) describe contributions of black grandmothers and detect obstacles that limit their success; and (c) recommend curricula for programs to enhance success of black grandmothers. The 253 subjects were black grandmothers (n=76),…

Toward Equity for Blacks in Higher Education.

ERIC Educational Resources Information Center

Lyons, Charles A.

The present condition of the United States regarding the education of black college-age youth and the status of historically black colleges is discussed in this paper. Background information on the role of black colleges and an elaboration of present-day problems facing black colleges are presented. The declining enrollment, drop-out rate, counter…

Uneven Stories: Successful Black Collegians at a Black and a White Campus.

ERIC Educational Resources Information Center

Fries-Britt, Sharon L.; Turner, Bridget

2002-01-01

Extensive interviews with academically successful Black students attending a traditionally White and a historically Black institution produced two themes. The first concerns differences in support and campus involvement, and the second reveals how the energy of Black students is either cultivated by their connections with peers and faculty or…

Black Films in Search of a Home.

ERIC Educational Resources Information Center

Taylor, Clyde

1983-01-01

Black filmmakers have produced many films that successfully showcase African-American culture, yet this work is rarely exposed to its intended audience. The Black bourgeoisie, because of their money and social status, must play a role in supporting Black filmmaking and fostering the perception of Black film as part of American popular culture. (GC)

50 CFR 648.146 - Black sea bass recreational fishing season.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass recreational fishing... Management Measures for the Black Sea Bass Fishery § 648.146 Black sea bass recreational fishing season... possession limit specified in § 648.145(a), may possess black sea bass from January 1 through February 28...

50 CFR 648.143 - Black sea bass Accountability Measures.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass Accountability Measures... Management Measures for the Black Sea Bass Fishery § 648.143 Black sea bass Accountability Measures. (a... based on dealer reports, state data, and other available information. All black sea bass landed for sale...

75 FR 59154 - Fisheries of the Northeastern United States; Black Sea Bass Fishery; 2010 Black Sea Bass...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

.... 100120036-0360-02] RIN 0648-XT99 Fisheries of the Northeastern United States; Black Sea Bass Fishery; 2010 Black Sea Bass Specifications; Emergency Rule Extension; Correction AGENCY: National Marine Fisheries... emergency action to increase the 2010 black sea bass specifications. The preamble text of that rule...

75 FR 38935 - Fisheries of the Northeastern United States; Black Sea Bass Fishery; 2010 Black Sea Bass...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-07

...--XT99 [Docket No. 100120036-0038-01] Fisheries of the Northeastern United States; Black Sea Bass Fishery; 2010 Black Sea Bass Specifications; Emergency Rule Extension AGENCY: National Marine Fisheries Service...; emergency action extension. SUMMARY: NMFS is extending the emergency action to increase the 2010 black sea...

50 CFR 648.143 - Black sea bass Accountability Measures.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Black sea bass Accountability Measures... Management Measures for the Black Sea Bass Fishery § 648.143 Black sea bass Accountability Measures. (a... based on dealer reports, state data, and other available information. All black sea bass landed for sale...

76 FR 53831 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-30

... Black Sea Bass Fisheries; 2011 Summer Flounder, Scup, and Black Sea Bass Specifications; Correction... Federal Register the final rule to implement the 2011 summer flounder, scup, and black sea bass....100. Need for Correction The final rule implementing 2011 summer flounder, scup, and black sea bass...

77 FR 24151 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-23

... Black Sea Bass Fisheries; Final 2012 Summer Flounder, Scup, and Black Sea Bass Specifications AGENCY... management measures, for the 2012 summer flounder, scup, and black sea bass fisheries. The specifications are... specifications for the summer flounder, scup, and black sea bass fisheries, including commercial quotas...

50 CFR 648.144 - Black sea bass gear restrictions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass gear restrictions. 648.144... Measures for the Black Sea Bass Fishery § 648.144 Black sea bass gear restrictions. (a) Trawl gear restrictions—(1) General. (i) Otter trawlers whose owners are issued a black sea bass moratorium permit and...

50 CFR 648.143 - Black sea bass Accountability Measures.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass Accountability Measures... Management Measures for the Black Sea Bass Fishery § 648.143 Black sea bass Accountability Measures. (a... based on dealer reports, state data, and other available information. All black sea bass landed for sale...

50 CFR 648.144 - Black sea bass gear restrictions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Black sea bass gear restrictions. 648.144... Measures for the Black Sea Bass Fishery § 648.144 Black sea bass gear restrictions. (a) Trawl gear restrictions—(1) General. (i) Otter trawlers whose owners are issued a black sea bass moratorium permit and...

50 CFR 648.144 - Black sea bass gear restrictions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass gear restrictions. 648.144... Measures for the Black Sea Bass Fishery § 648.144 Black sea bass gear restrictions. (a) Trawl gear restrictions—(1) General. (i) Otter trawlers whose owners are issued a black sea bass moratorium permit and...

77 FR 76950 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-31

... Black Sea Bass Fisheries; 2012 Summer Flounder, Scup, and Black Sea Bass Specifications; Correction... Register the final rule to implement the 2012 summer flounder, scup, and black sea bass specifications... summer flounder, scup, and black sea bass specifications. Regulations for the summer flounder fishery are...

The Thermodynamics of Black Holes.

PubMed

Wald, Robert M

2001-01-01

We review the present status of black hole thermodynamics. Our review includes discussion of classical black hole thermodynamics, Hawking radiation from black holes, the generalized second law, and the issue of entropy bounds. A brief survey also is given of approaches to the calculation of black hole entropy. We conclude with a discussion of some unresolved open issues.

Black Male/Female Conflict: Internalization of Negative Definitions Transmitted Through Imagery.

ERIC Educational Resources Information Center

Jewell, K. Sue

1983-01-01

Examines negative images of Black men and women in American society and contrasts these with positive images of Whites. Suggests that the attribution of stereotyped, negative characteristics to Blacks has affected Blacks' perceptions of self-worth and has resulted in interpersonal conflict between Black males and females. (Author/MJL)

Higher Education Equity: The Crisis of Appearance Versus Reality--Revisited. Final Report.

ERIC Educational Resources Information Center

National Advisory Committee on Black Higher Education and Black Colleges and Universities (DHEW), Washington, DC.

The work of the National Advisory Committee on Black Higher Education and Black Colleges and Universities during 1977-1982 is discussed, and information and recommendations on black students and black colleges are presented. After describing the committee's background and functions, the following issues concerning equity for black Americans in…

20 CFR 725.1 - Statutory provisions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Federal Coal Mine Health and Safety Act of 1969, as amended by the Black Lung Benefits Act of 1972, the Federal Mine Safety and Health Amendments Act of 1977, the Black Lung Benefits Reform Act of 1977, the Black Lung Benefits Revenue Act of 1977, the Black Lung Benefits Amendments of 1981, the Black Lung...

30 CFR 57.6901 - Black powder.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Black powder. 57.6901 Section 57.6901 Mineral...-Surface and Underground § 57.6901 Black powder. (a) Black powder shall be used for blasting only when a... dimension stone. (b) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed...

30 CFR 56.6901 - Black powder.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Black powder. 56.6901 Section 56.6901 Mineral....6901 Black powder. (a) Black powder shall be used for blasting only when a desired result cannot be...) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed cargo space while being...

30 CFR 56.6901 - Black powder.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Black powder. 56.6901 Section 56.6901 Mineral....6901 Black powder. (a) Black powder shall be used for blasting only when a desired result cannot be...) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed cargo space while being...

30 CFR 57.6901 - Black powder.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Black powder. 57.6901 Section 57.6901 Mineral...-Surface and Underground § 57.6901 Black powder. (a) Black powder shall be used for blasting only when a... dimension stone. (b) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed...

Guide to the Black Novel.

ERIC Educational Resources Information Center

Lederer, Richard

1969-01-01

The nature of black literature raises questions about a black aesthetic and the universality of black expression. Central in the writings of Richard Wright and Ralph Ellison are the black man's confusion of identity, stemming from his invisibility in a white America, and the crimes of ignorance and blindness perpetrated on him by whites and by…

Is It because I'm Black? A Black Female Research Experience

ERIC Educational Resources Information Center

Maylor, Uvanney

2009-01-01

This article examines what it means to be a Black female researcher in contemporary Britain. Drawing on Black feminist theory and critical race theory (CRT), this article seeks to highlight some of the experiences and challenges that Black female researchers face when undertaking research, particularly research that has diversity, equality or…

Reflections on the Black Woman's Role in the Community of Slaves.

ERIC Educational Resources Information Center

Davis, Angela Y.

1981-01-01

Reprint of a 1971 article that criticizes the notion of Black matriarchy as implying that Black women actively assented to slavery. Discusses Black resistance to slavery, especially among women, and stresses the importance of recognizing such resistance if current popular sociological views on Black women are to be revised. (GC)

The Black Community: Diversity and Unity.

ERIC Educational Resources Information Center

Blackwell, James E.

The black community is defined in this book as a diversified set of interrelated structures and aggregates of people who are held together by the forces of racism. The need for theoretical perspectives for understanding black-white relations and for analyzing the nature of the black community is explained. The black family, patterns of family…

Black Elite: The New Market for Highly Educated Black Americans.

ERIC Educational Resources Information Center

Freeman, Richard B.

This examination of the collapse in traditional discriminatory patterns in the market for highly qualified black Americans documents the World War II gain of college trained and related high level black workers, investigates the response of black college students and qualified personnel to the new market setting, and explores the factors that…

Finding the People's Ideology; Black Theatre at Brown University.

ERIC Educational Resources Information Center

Jones, Rhett S.

The author states that the black revolution went awry because black intellectuals failed to develop what has often been called a black political psychology, that is, the realization of a link between identity and polity, between black self-perspective and political actions; and that the individualistic emphasis of American life is causing upwardly…

A Group Counseling Model for Developing Manhood among Black Male Adolescents.

ERIC Educational Resources Information Center

Lee, Courtland

1991-01-01

A dialogue is presented, which highlights the meaning of success as a Black man. C. Lee's Black Manhood Training Model is discussed, which was developed as a group counseling experience in a school setting for 13- to 17-year-old Black boys to address the challenges of Black male development. (SLD)

The Black Female Experience in America: A Learning/Teacher Guide.

ERIC Educational Resources Information Center

Allman, Joanna; And Others

This book provides a guide for teaching and learning about the experiences of Black females in America, and particularly about psychosocial issues in Black female development. The guide is divided into topic units. "Black Women in History" contains lessons of Black women in slavery, in the twentieth century, and historical and contemporary role…

The Black Man in American Society.

ERIC Educational Resources Information Center

Framingham Public Schools, MA.

GRADE OR AGES: Junior high school. SUBJECT MATTER: The black man in American society. ORGANIZATION AND PHYSICAL APPEARANCE: There are four major parts each with an overview. The four parts concern a) the African heritage of the black man, b) the American exploitation of the black man, c) the black man's contribution to American society, d) the…

30 CFR 57.6901 - Black powder.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Black powder. 57.6901 Section 57.6901 Mineral...-Surface and Underground § 57.6901 Black powder. (a) Black powder shall be used for blasting only when a... dimension stone. (b) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed...

30 CFR 57.6901 - Black powder.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Black powder. 57.6901 Section 57.6901 Mineral...-Surface and Underground § 57.6901 Black powder. (a) Black powder shall be used for blasting only when a... dimension stone. (b) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed...

30 CFR 56.6901 - Black powder.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Black powder. 56.6901 Section 56.6901 Mineral....6901 Black powder. (a) Black powder shall be used for blasting only when a desired result cannot be...) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed cargo space while being...

30 CFR 57.6901 - Black powder.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Black powder. 57.6901 Section 57.6901 Mineral...-Surface and Underground § 57.6901 Black powder. (a) Black powder shall be used for blasting only when a... dimension stone. (b) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed...

30 CFR 56.6901 - Black powder.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Black powder. 56.6901 Section 56.6901 Mineral....6901 Black powder. (a) Black powder shall be used for blasting only when a desired result cannot be...) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed cargo space while being...

30 CFR 56.6901 - Black powder.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Black powder. 56.6901 Section 56.6901 Mineral....6901 Black powder. (a) Black powder shall be used for blasting only when a desired result cannot be...) Containers of black powder shall be— (1) Nonsparking; (2) Kept in a totally enclosed cargo space while being...

Communication Apprehension among Black Students on Predominantly White Campuses.

ERIC Educational Resources Information Center

Byrd, Marquita L.; Sims, Anntarie L.

1987-01-01

A study of 114 Black undergraduates in two predominantly White midwestern universities demonstrates that communication apprehension (CA) among Blacks appears to be an audience-based phenomenon. Black females scored lower than Black males on the Personal Report of Communication Apprehension-24 (PRCA-24). The higher the CA score, the higher the…

Problems of Dual-Career Black Couples: Identification and Implications for Family Interventions.

ERIC Educational Resources Information Center

Thomas, Veronica G.

1990-01-01

Examined problems of 41 dual-career Black couples. Findings indicated that dual-career Black couples experienced a number of problems related to their lifestyle. Found unique problems perceived by dual-career Black families. In addition to problems experienced by White dual-career couples, Blacks also faced racial discrimination, social isolation,…

Complicated Contradictions Amid Black Feminism and Millennial Black Women Teachers Creating Curriculum for Black Girls

ERIC Educational Resources Information Center

Nyachae, Tiffany M.

2016-01-01

Millennial Black women teachers wrestle with two simultaneous burdens: disrupting the racist and sexist status quo of schooling through curriculum, and employing tactics to survive school politics among their majority White women colleagues. This article describes how the "Sisters of Promise" (SOP) curriculum aligned with Black feminism…

Who's Afraid of the Big Black Man?

ERIC Educational Resources Information Center

Johnson, Jason Kyle

2018-01-01

This article examines the experiences of big Black men in both their personal and professional lives. Black men are often perceived as being aggressive, violent, and physically larger than their White counterparts. The negative perceptions of Black men, particularly big Black men, often leads to negative encounters with police, educators, and…

Exploring Sense of Belonging among Black International Students at an HBCU

ERIC Educational Resources Information Center

Mwangi, Chrystal A. George

2016-01-01

This study elucidates the experiences of HBCU (historically Black colleges and universities) students who are racially Black, but differ in nativity and nationality from their Black American peers. The purpose is to examine Black HBCU international students' sense of belonging on campus. This study engages qualitative individual interviews with…

Graduating Black Males: A Generic Qualitative Study

ERIC Educational Resources Information Center

Bell, Edward E.

2014-01-01

Black males face a difficult educational battle. Across America, graduation statistics for Black males are sobering. The purpose of this study was to explore why Black males drop out of school and to examine the current employment status of the study participants. The research took place in rural North Carolina. Fifteen Black American male high…

Black Mathematics Educators: Researching toward Racial Emancipation of Black Students

ERIC Educational Resources Information Center

Ridgeway, Monica L.; McGee, Ebony O.

2018-01-01

This article focuses on the scholarship of Black mathematics education researchers whose work focuses on Black students in P-20 mathematics spaces. We conducted a metasynthesis literature review of empirical studies by Black mathematics education researchers. The authors utilized critical theories of race and racism to aid in the synthesis of the…

Resisting the Status Quo: The Narratives of Black Homeschoolers in Metro-Atlanta and Metro-DC

ERIC Educational Resources Information Center

Fields-Smith, Cheryl; Kisura, Monica Wells

2013-01-01

Trends suggest that homeschooling continues to increase among black families. Yet, research on contemporary Black homeschooling remains scarce. Given black educational history, the phenomena of Black families choosing homeschooling over public and private schools in the post-Desegregation era is worthy of investigation. Further, documenting the…

Advancing Black Male Success: Understanding the Contributions of Urban Black Colleges and Universities

ERIC Educational Resources Information Center

Gasman, Marybeth; Nguyen, Thai-Huy; Commodore, Felecia

2017-01-01

To understand the context of urban Historically Black Colleges and Universities (HBCUs) and their role in educating Black males, we conducted a literature review examining the academic contributions of these institutions to Black males. To bolster the literature, we examined Integrated Postsecondary Education Data System data, determining a set of…

School Composition and the Black-White Achievement Gap. NCES 2015-018

ERIC Educational Resources Information Center

Bohrnstedt, G.; Kitmitto, S.; Ogut, B.; Sherman, D.; Chan, D.

2015-01-01

School Composition and the Black-White Achievement Gap explores public schools' demographic composition, in particular, the proportion of Black students enrolled in schools (also referred to "Black student density" in schools) and its relation to the Black-White achievement gap. This NCES study, the first of its kind, used the 2011 NAEP…

Black holes

PubMed Central

Brügmann, B.; Ghez, A. M.; Greiner, J.

2001-01-01

Recent progress in black hole research is illustrated by three examples. We discuss the observational challenges that were met to show that a supermassive black hole exists at the center of our galaxy. Stellar-size black holes have been studied in x-ray binaries and microquasars. Finally, numerical simulations have become possible for the merger of black hole binaries. PMID:11553801

Black holes.

PubMed

Brügmann, B; Ghez, A M; Greiner, J

2001-09-11

Recent progress in black hole research is illustrated by three examples. We discuss the observational challenges that were met to show that a supermassive black hole exists at the center of our galaxy. Stellar-size black holes have been studied in x-ray binaries and microquasars. Finally, numerical simulations have become possible for the merger of black hole binaries.

Crisis of Black Athletes on the Eve of the 21st Century.

ERIC Educational Resources Information Center

Edwards, Harry

2000-01-01

Asserts that the dynamics of black youth sports involvement and the blind faith of black youths and families in sports as a vehicle for self-realization and socioeconomic advancement generate complex problems for black society. Black families often push their children toward sports careers, neglecting personal and cultural development. Discusses…

The Core Journal Concept in Black Studies

ERIC Educational Resources Information Center

Weissinger, Thomas

2010-01-01

Black Studies scholars have shown interest in the core journal concept. Indeed, the idea of core journals for the study of the Black experience has changed several times since 1940. While Black Studies scholars are citing Black Studies journals with frequency, they also cite traditional disciplinary journals a great deal of the time. However,…

Black and White College Women's Perceptions of Sexual Harassment.

ERIC Educational Resources Information Center

Shelton, J. Nicole; Chavous, Tabbye M.

1999-01-01

Examined how racial factors influence college women's perceptions of sexual harassment with samples of 46 black and 89 white women. Data suggest that sexual harassment between black women and black men is trivialized compared to sexual behavior between black women and white men. Discusses implications for the study of sexual harassment. (SLD)

Black Women in Academe. Issues and Strategies.

ERIC Educational Resources Information Center

Moses, Yolanda T.

The climate for black women students, faculty members, and administrators in both predominantly white as well as historically black colleges and universities is explored, focusing on the subtle and not so subtle ways that race and gender stereotypes can combine to create double obstacles for black women. Black women students, faculty members, and…

Black Views of American Women: The View From Black Newspapers, 1865-1900.

ERIC Educational Resources Information Center

Beatty, Bess

Although black journalists from 1865 to 1900 were more sensitive to stereotyping and discrimination than their white counterparts, the black papers approached women idealistically, rather than through the realistic situation in which black women existed or through their own awareness of the fact of oppression. The images and proscriptions of women…

The Afro-American Family.

ERIC Educational Resources Information Center

Alexander, Benjamin H.

If the black family is to survive, blacks must go into the community, seek out their poor, teach them, minister to them, and Work with them to solve their problems. Black youth, in particular, need support and encouragement. The most successful black children come from two-parent homes. Hence the percentage of black families--35%--headed by a…

Hairy black holes and the endpoint of AdS4 charged superradiance

NASA Astrophysics Data System (ADS)

Dias, Óscar J. C.; Masachs, Ramon

2017-02-01

We construct hairy black hole solutions that merge with the anti-de Sitter (AdS4) Reissner-Nordström black hole at the onset of superradiance. These hairy black holes have, for a given mass and charge, higher entropy than the corresponding AdS4-Reissner-Nordström black hole. Therefore, they are natural candidates for the endpoint of the charged superradiant instability. On the other hand, hairy black holes never dominate the canonical and grand-canonical ensembles. The zero-horizon radius of the hairy black holes is a soliton (i.e. a boson star under a gauge transformation). We construct our solutions perturbatively, for small mass and charge, so that the properties of hairy black holes can be used to testify and compare with the endpoint of initial value simulations. We further discuss the near-horizon scalar condensation instability which is also present in global AdS4-Reissner-Nordström black holes. We highlight the different nature of the near-horizon and superradiant instabilities and that hairy black holes ultimately exist because of the non-linear instability of AdS.

The MaBwana Black men's study: community and belonging in the lives of African, Caribbean and other Black gay men in Toronto.

PubMed

George, Clemon; Adam, Barry A; Read, Stanley E; Husbands, Winston C; Remis, Robert S; Makoroka, Lydia; Rourke, Sean B

2012-01-01

In Canada, there is a paucity of research aimed at understanding Black gay men and the antecedents to risk factors for HIV. This study is an attempt to move beyond risk factor analysis and explore the role of sexual and ethnic communities in the lives of these men. The study utilized a community-based research and critical race theory approach. Semi-structured interviews were conducted with eight key informants to augment our understanding of Black gay men and to facilitate recruitment of participants. In-depth interviews were done with 24 Black gay men. Our data showed that the construction of community for Black gay men is challenged by their social and cultural environment. However, these men use their resilience to navigate gay social networks. Black gay men expressed a sense of abjuration from both gay and Black communities because of homophobia and racism. It is essential for health and social programmers to understand how Black gay men interact with Black and gay communities and the complexities of their interactions in creating outreach educational, preventive and support services.

Ethnicity, Nativity and the Health of American Blacks

PubMed Central

Griffith, Derek M.; Johnson, Jonetta; Zhang, Rong; Neighbors, Harold W.; Jackson, James S.

2012-01-01

Few national studies have empirically examined ethnic differences in health within the American Black population. We utilized logistic regression to examine the relationships among ethnicity, nativity, depressive symptoms, and physical health in the two largest ethnic groups of American Blacks, African Americans and Caribbean Blacks. The data were from the National Survey of American Life, an in-person national household survey representative of the non-institutionalized U.S. Black population. We found that African Americans, U.S.-born Caribbean Blacks, and Caribbean-born Blacks had significantly different self-ratings of their health and self-reports of being diagnosed with a chronic physical health condition. Whether assessed by self-rated health or the presence of at least one physician diagnosed chronic health condition, Caribbean-born Blacks had the best health outcomes and U.S.-born Caribbean Blacks had the worst. This finding remained significant even after considering self-reported depressive symptoms. This study highlights the importance of considering ethnic diversity, nativity and immigration as independent sources of variation in health status within the American Black population. PMID:21317512

Relation between vegetarian/nonvegetarian diets and blood pressure in black and white adults.

PubMed Central

Melby, C L; Goldflies, D G; Hyner, G C; Lyle, R M

1989-01-01

We examined the possible interaction of race and diet on blood pressure (BP) in volunteer Black Seventh Day Adventists compared to volunteer White church members. Height, weight, waist and hip circumference, and resting seated BP were recorded in Black vegetarians (n = 55; age: 54.7 +/- 16.9 yrs), Black nonvegetarians (n = 59; 56.1 +/- 14.1 yrs), White vegetarians (n = 164; 52.2 +/- 16.7 yrs), and White nonvegetarians (n = 100; 52.6 +/- 15.6 yrs) attending a regional conference. Forty-four percent of the Black nonvegetarians were medicated hypertensives, compared to only 18 percent of the Black vegetarians, 7 percent of the White vegetarians, and 22 percent of the White nonvegetarians. Black vegetarians exhibited lower age and sex-adjusted systolic BP (means = 122.9/74.4 mm Hg) than Black nonvegetarians (means = 132.2/75.9 mm Hg). After further adjusting BP for body mass index and waist/hip ratio, the systolic BP among Black vegetarians remained lower (122.8) than Black nonvegetarians (129.7) but higher than that of the Whites who showed no diet-related BP differences. PMID:2764208

Supermassive black holes do not correlate with dark matter haloes of galaxies.

PubMed

Kormendy, John; Bender, Ralf

2011-01-20

Supermassive black holes have been detected in all galaxies that contain bulge components when the galaxies observed were close enough that the searches were feasible. Together with the observation that bigger black holes live in bigger bulges, this has led to the belief that black-hole growth and bulge formation regulate each other. That is, black holes and bulges coevolve. Therefore, reports of a similar correlation between black holes and the dark matter haloes in which visible galaxies are embedded have profound implications. Dark matter is likely to be non-baryonic, so these reports suggest that unknown, exotic physics controls black-hole growth. Here we show, in part on the basis of recent measurements of bulgeless galaxies, that there is almost no correlation between dark matter and parameters that measure black holes unless the galaxy also contains a bulge. We conclude that black holes do not correlate directly with dark matter. They do not correlate with galaxy disks, either. Therefore, black holes coevolve only with bulges. This simplifies the puzzle of their coevolution by focusing attention on purely baryonic processes in the galaxy mergers that make bulges.

Black widow spider

MedlinePlus

... medlineplus.gov/ency/article/002858.htm Black widow spider To use the sharing features on this page, please enable JavaScript. The black widow spider (Latrodectus) has a shiny black body with a ...

Black Contributions to the Early History of Western Medicine: Lack of Recognition as a Cause of Black Under-Representation in US Medical Schools

PubMed Central

Newsome, Frederick

1979-01-01

During several millenia, blacks in ancient Egypt made numerous contributions to medicine and were acknowledged as the inventors of the art of medicine. They produced the earliest physicians, medical knowledge, and medical literature. They contributed to the development of medicine in ancient Greece. Ancient writers, including Herodotus, Isocrates, and Diodorus, affirm this. Modern presentations of ancient medicine, however, deprive blacks of the knowledge of their early contributions to medicine by ignoring or subtly misrepresenting the black identity of the ancient Egyptians. Blacks are currently under-represented in US medical schools. It is proposed that the recognition of the contributions of blacks to the early history of Western medicine would inspire black students to study medicine. PMID:423296

PREJUDICE & POLICY Racial Discrimination in the Union Army Disability Pension System, 1865-1906

PubMed Central

2010-01-01

The Union Army disability pension was an early experiment in colorblind social policy. However, it shortchanged Blacks in 2 ways. First, the law was unable to account for the challenges Blacks faced in proving their eligibility because of the legacy of slavery and discrimination against Black troops during the Civil War. Second, the increasing leniency accorded White soldiers by the Pension Bureau was not extended in the same measure to Blacks. Active discrimination against Blacks resulted in part from local discretion, evidenced by the significantly lower approval rates for both White and Black veterans in the South. Furthermore, when Whites and Blacks claimed disabilities that were easily verifiable, outcomes were similar, but when verification required a degree of trust, Blacks fared considerably worse than Whites. PMID:20147675

The Longue Durée of Black Lives Matter.

PubMed

Nelson, Alondra

2016-10-01

Black Lives Matter was first articulated just a few years ago, but it has been the leitmotif of antiracist struggles for generations. The Movement for Black Lives extends the work of previous movements that challenged forms of oppression that act on Black bodies with impunity. It should be understood in the context of Ida B. Wells' anti-lynching campaign, Fannie Lou Hamer's reproductive justice demands, and the Black Panther Party's health activism. The 50th anniversary of the Black Panther Party is an occasion to recall that its work confronted the callous neglect and the corporeal surveillance and abuse of poor Black communities. Similar demands have been the centrifugal force of social movements that for centuries have refused to have Black lives cast beyond the human boundary.

The Longue Durée of Black Lives Matter

PubMed Central

2016-01-01

Black Lives Matter was first articulated just a few years ago, but it has been the leitmotif of antiracist struggles for generations. The Movement for Black Lives extends the work of previous movements that challenged forms of oppression that act on Black bodies with impunity. It should be understood in the context of Ida B. Wells’ anti-lynching campaign, Fannie Lou Hamer’s reproductive justice demands, and the Black Panther Party’s health activism. The 50th anniversary of the Black Panther Party is an occasion to recall that its work confronted the callous neglect and the corporeal surveillance and abuse of poor Black communities. Similar demands have been the centrifugal force of social movements that for centuries have refused to have Black lives cast beyond the human boundary. PMID:27626337

Cigarette advertising in Black, Latino, and White magazines, 1998-2002: an exploratory investigation.

PubMed

Landrine, Hope; Klonoff, Elizabeth A; Fernandez, Senaida; Hickman, Norval; Kashima, Kennon; Parekh, Bina; Thomas, KaMala; Brouillard, Catherine R; Zolezzi, Michelle; Jensen, Jennifer A; Weslowski, Zorahna

2005-01-01

To examine the number, type (menthol vs non-menthol), brand (Black, White, Women's, Other), and size of cigarette ads in Black, Latino, and White magazines. Analysis of digital photographs of 274 cigarette ads appearing in Ebony (Black), People (White), and People in Spanish (Latino) for the 4.5-year period of January 1998 to August 2002. Black magazines were 9.8 times and Latino magazines 2.6 times more likely than White magazines to contain ads for menthol cigarettes. Black and Latino magazines also contained significantly more ads for brands (Virginia Slims) that target women. The tobacco industry continues to target Blacks with menthol cigarette ads, appears now to be targeting Latinos similarly, and targets Black and Latino women with additional, tailored cigarette ads.

Exposure of black youths to cigarette advertising in magazines

PubMed Central

King, C.; Siegel, M.; Pucci, L.

2000-01-01

OBJECTIVE—To estimate the potential exposure of black adolescents to brand specific advertising in magazines.
DESIGN—A probit regression analysis was conducted of pooled 1990 and 1994 data on brand specific advertising in 36 popular US magazines to examine the relationship between the presence or absence of advertising in each magazine for each of 12 cigarette brands, and the proportion of each magazine's youth (ages 12-17 years) readers who were black.
MAIN OUTCOME MEASURES—The presence or absence of advertising in each magazine in 1990 and 1994, for each of 12 cigarette brands.
RESULTS—After controlling for total magazine readership and the percentage of young adult, Hispanic, and female readers, black youth cigarette brands (those whose market share among black youths exceeded their overall market share) were more likely than other brands to advertise in magazines with a higher percentage of black youth readers. Holding all other variables constant at their sample means, the probability of a non-black youth brand advertising in a magazine decreased over the observed range of percentage black youth readership from 0.65 (95% confidence interval (CI) 0.55 to 0.75) for magazines with 5% black youth readers to 0.33 (95% CI 0.00 to 0.69) for magazines with 91% black youth readers. In contrast, the probability of a black youth brand advertising in a magazine increased from 0.40 (95% CI 0.17 to 0.62) at 5% black youth readership to 1.00 (95% CI 0.97 to 1.00) at 91% black youth readership.
CONCLUSIONS—Black youths are more likely than white youths to be exposed to magazine advertising by cigarette brands popular among black adolescents.


Keywords: advertising; magazines; youths PMID:10691759

Black holes in binary stellar systems and galactic nuclei

NASA Astrophysics Data System (ADS)

Cherepashchuk, A. M.

2014-04-01

In the last 40 years, following pioneering papers by Ya B Zeldovich and E E Salpeter, in which a powerful energy release from nonspherical accretion of matter onto a black hole (BH) was predicted, many observational studies of black holes in the Universe have been carried out. To date, the masses of several dozen stellar-mass black holes (M_BH = (4{-}20) M_\\odot) in X-ray binary systems and of several hundred supermassive black holes (M_BH = (10^{6}{-}10^{10}) M_\\odot) in galactic nuclei have been measured. The estimated radii of these massive and compact objects do not exceed several gravitational radii. For about ten stellar-mass black holes and several dozen supermassive black holes, the values of the dimensionless angular momentum a_* have been estimated, which, in agreement with theoretical predictions, do not exceed the limiting value a_* = 0.998. A new field of astrophysics, so-called black hole demography, which studies the birth and growth of black holes and their evolutionary connection to other objects in the Universe, namely stars, galaxies, etc., is rapidly developing. In addition to supermassive black holes, massive stellar clusters are observed in galactic nuclei, and their evolution is distinct from that of supermassive black holes. The evolutionary relations between supermassive black holes in galactic centers and spheroidal stellar components (bulges) of galaxies, as well as dark-matter galactic haloes are brought out. The launch into Earth's orbit of the space radio interferometer RadioAstron opened up the real possibility of finally proving that numerous discovered massive and highly compact objects with properties very similar to those of black holes make up real black holes in the sense of Albert Einstein's General Relativity. Similar proofs of the existence of black holes in the Universe can be obtained by intercontinental radio interferometry at short wavelengths \\lambda \\lesssim 1 mm (the international program, Event Horizon Telescope).

Sizes of Black Holes Throughout the Universe

NASA Astrophysics Data System (ADS)

Kohler, Susanna

2018-05-01

What is the distribution of sizes of black holes in our universe? Can black holes of any mass exist, or are there gaps in their possible sizes? The shape of this black-hole mass function has been debated for decades and the dawn of gravitational-wave astronomy has only spurred further questions.Mind the GapsThe starting point for the black-hole mass function lies in the initial mass function (IMF) for stellar black holes the beginning size distribution of black holes after they are born from stars. Instead of allowing for the formation of stellar black holes of any mass, theoretical models propose two gaps in the black-hole IMF:An upper mass gap at 50130 solar masses, due to the fact that stellar progenitors of black holes in this mass range are destroyed by pair-instability supernovae.A lower mass gap below 5 solar masses, which is argued to arise naturally from the mechanics of supernova explosions.Missing black-hole (BH) formation channels due to the existence of the lower gap (LG) and the upper gap (UG) in the initial mass function. a) The number of BHs at all scales are lowered because no BH can merge with BHs in the LG to form a larger BH. b) The missing channel responsible for the break at 10 solar masses, resulting from the LG. c) The missing channel responsible for the break at 60 solar masses, due to the interaction between the LG and the UG. [Christian et al. 2018]We can estimate the IMF for black holes by scaling a typical IMF for stars and then adding in these theorized gaps. But is this initial distribution of black-hole masses the same as the distribution that we observe in the universe today?The Influence of MergersBased on recent events, the answer appears to be no! Since the first detections of gravitational waves in September 2015, we now know that black holes can merge to form bigger black holes. An initial distribution of black-hole masses must therefore evolve over time, as mergers cause the depletion of low-mass black holes and an increase in higher-mass black holes.A team of scientists led by Pierre Christian, an Einstein Fellow at Harvard University, has now looked into characterizing this shift. In particular, Christian and collaborators explore how black-hole mergers in the centers of dense star clustersultimately shape the black-hole mass function of the universe.Black Holes TodayChristian and collaborators use analytical models of coagulation mergers of particles to form larger particles to estimate the impact of mergers in star clusters on resulting black-hole sizes. They find that, over an evolution of 10 billion years, mergers can appreciably fill in the upper mass gap of the black-hole IMF.An example of the black-hole mass function that can result from evolving the initial mass function complete with gaps over time. Two breaks appear as a result of the initial gaps: one at 10 (LB) and one at 60 solar masses (UB). [Christian et al. 2018]The lower mass gap, on the other hand, leaves observable signatures in the final black-hole mass function: a break at 10 solar masses (since black holes below this mass cant be created by mergers) and one at 60 solar masses (caused by the interaction of the upper and lower gaps). As we build up black-hole statistics in the future (thanks, gravitational-wave detectors!), searching for these breaks will help us to test our models.Lastly, the authors find that their models can only be consistent with observations if ejection is efficient black holes must be regularly ousted from star clusters through interactions with other bodies or as a result of kicks when they merge. This idea is consistent with many recent studies supporting a large population of free-floating stellar-mass black holes.CitationPierre Christian et al 2018 ApJL 858 L8. doi:10.3847/2041-8213/aabf88

Mass-induced instability of SAdS black hole in Einstein-Ricci cubic gravity

NASA Astrophysics Data System (ADS)

Myung, Yun Soo

2018-05-01

We perform the stability analysis of Schwarzschild-AdS (SAdS) black hole in the Einstein-Ricci cubic gravity. It shows that the Ricci tensor perturbations exhibit unstable modes for small black holes. We call this the mass-induced instability of SAdS black hole because the instability of small black holes arises from the massiveness in the linearized Einstein-Ricci cubic gravity, but not a feature of higher-order derivative theory giving ghost states. Also, we point out that the correlated stability conjecture holds for the SAdS black hole by computing the Wald entropy of SAdS black hole in Einstein-Ricci cubic gravity.

Hawking Tunneling Radiation of Black Holes in de Sitter and ANTI-de Sitter Spacetimes

NASA Astrophysics Data System (ADS)

Jiang, Qing-Quan; Li, Hui-Ling; Yang, Shu-Zheng; Chen, De-You

Applying Parikh-Wilczek's semiclassical quantum tunneling method, we investigate the tunneling radiation characteristics of a torus-like black hole and Kerr-Newman-Kausya de Sitter black hole. Both black holes have the cosmological constant Λ, but a torus-like black hole is in anti-de Sitter spacetime and the other black hole is in de Sitter spacetime. The derived results show that the tunneling rate is related to the change of Bekenstein-Hawking entropy, and the factual radiated spectrum is not precisely thermal, but is consistent with an underlying unitary theory, which gives a might explanation to the paradox of black hole information lost.

How Black women make sense of 'White' and 'Black' fashion magazines: a qualitative think aloud study.

PubMed

Ogden, Jane; Russell, Sheriden

2013-12-01

This qualitative think aloud study explored how Black women (n = 32) processed information from a White or Black fashion magazine. Comments to the 'White' magazine were characterised by rejection, being critical of the media and ambivalence, whereas they responded to the 'Black' magazine with celebration, identification and a search for depth. Transcending these themes was their self-identity of being a Black woman that was brought to the fore either by a sense of exclusion (White magazine) or engagement (Black magazine). Such an identity provides resilience against the media's thin ideals by minimising the processes of social comparison and internalisation.

Black-on-black homicide: Kansas City's response.

PubMed Central

Mitchell, M A; Daniels, S

1989-01-01

In many metropolitan areas, homicide continues to be the scourge of black Americans despite increasing awareness of the overrepresentation of blacks among victims and perpetrators. The risk of being a homicide victim among black males is so high that the Department of Health and Human Services has set a priority of reducing the risk to 60 per 100,000 by 1990. The recent escalation in the number of homicides in the United States associated with drugs makes attainment of that goal unlikely. In Kansas City, a black community grassroots organization, the Ad Hoc Group Against Crime, commissioned a multidisciplinary task force to study black-on-black homicide in 1986. The report generated by this task force identified factors placing Kansas Citians at high risk of being homicide victims or perpetrators, including being black, male, unemployed, between the ages 17-29, a high school nongraduate, frequently involved in or around violence, and having prior arrests on weapons charges. One hundred recommendations were made, of which 12 were targeted for immediate implementation. These included increasing public awareness of the incidence of black-on-black homicide, involvement of black men in role model programs for young black males, training in anger control and alternatives to violence for those identified as being at high risk for homicide, and providing a role for ex-offenders in violence prevention. Working with community organizations has inherent strengths and weaknesses for public health workers. However, such a group can successfully impact the affected community in ways which would be difficult for traditional resources. PMID:2511593

Black Carbon Measurement and Modeling in the Arabian Peninsula

NASA Astrophysics Data System (ADS)

Zawad, Faisal Al; Khoder, Mamdouh; Almazroui, Mansour; Alghamdi, Mansour; Lihavainen, Heikki; Hyvarinen, Antti; Henriksson, Svante

2017-04-01

Black carbon is an important atmospheric aerosol as an effective factor in public health, changing the global and regional climate, and reducing visibility. Black carbon absorbs light, warms the atmosphere, and modifies cloud droplets and the amount of precipitation. In spite of this significance, knowledge of black carbon over the Arabian Peninsula is hard to find in literature until recently. The total mass of black carbon and wind direction and speeds were measured continuously at Hada Al-Sham, Saudi Arabia for the year 2013. In addition, a state of the art global aerosol - climate model (ECHAM5-HAM) was used to determine black carbon climatology over the Arabian Peninsula. Simulation of the model was carried out for the years eight years (2004 - 2011). The daily mean values of the concentrations of black carbon had a minimum of 15.0 ng/m3 and a maximum of 6372 ng/m3 with a mean of at 1899 ng/m3. The diurnal pattern of black carbon showed higher values overnight, and steady low values during daytimes caused by sea and land breezes. Seasons of black carbon vary over the Arabian Peninsula, and the longest is in the Northern Region where it lasts from July to October. High concentrations of black carbon at Hada Al-Sham was observed with a mean of 1.9 µm/m3, and seasons of black carbon vary widely across the Arabian Peninsula. Assessment of the effects of black carbon over the Arabian Peninsula on the global radiation balance. Initiating a black carbon monitoring network is highly recommended to assess its impacts on health, environment, and climate.

Reconceptualizing successful aging among black women and the relevance of the strong black woman archetype.

PubMed

Baker, Tamara A; Buchanan, NiCole T; Mingo, Chivon A; Roker, Rosalyn; Brown, Candace S

2015-02-01

Although there are multiple pathways to successful aging, little is known of what it means to age successfully among black women. There is a growing body of literature suggesting that black women experience a number of social challenges (sexism and racism) that may present as barriers to aging successfully. Applying aspects of the Strong Black Women ideal, into theoretical concepts of successful aging, may be particularly relevant in understanding which factors impair or promote the ability of black women to age successfully. The Strong Black Women archetype is a culturally salient ideal prescribing that black women render a guise of self-reliance, selflessness, and psychological, emotional, and physical strength. Although this ideal has received considerable attention in the behavioral sciences, it has been largely absent within the gerontology field. Nevertheless, understanding the dynamics of this cultural ideal may enhance our knowledge while developing an appreciation of the black woman's ability to age successfully. Rather than summarize the social, physical, and mental health literature focusing on health outcomes of black women, this conceptual review examines the Strong Black Women archetype and its application to the lived experiences of black women and contributions to current theories of successful aging. Focusing on successful aging exclusively among black women enhances our understanding of this group by considering their identity as women of color while recognizing factors that dictate their ability to age successfully. © The Author 2014. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

UH-60M Black Hawk Helicopter (UH-60M Black Hawk)

DTIC Science & Technology

2016-12-01

Selected Acquisition Report (SAR) RCS: DD-A&T(Q&A)823-341 UH-60M Black Hawk Helicopter (UH-60M Black Hawk) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 21, 2016 18:25:45 UNCLASSIFIED UH-60M Black Hawk December 2015 SAR March 21, 2016 18...Operational Requirements Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost UH-60M Black Hawk

Searching for Black Holes

NASA Technical Reports Server (NTRS)

Garica, M.

2001-01-01

In 1995 we proposed to carry out ground-based observations in order to securely identify stellar mass black holes in our galaxy. This type 4 proposal under NASA's UV, Visible, and Gravitational Astrophysics program compliments NASA's space-based research by following up black hole candidates found and studied with space-based observatories, in order to determine if they are indeed black holes. While our primary goal is to securely identify black holes by measuring their masses, a secondary goal is identifying unique visible-range signatures for black holes.

Black youth suicide: literature review with a focus on prevention.

PubMed Central

Baker, F. M.

1990-01-01

The national rates of completed suicide in the black population between 1950 and 1981 are presented, including age-adjusted rates. Specific studies of black suicide attempters and completed suicides by blacks in several cities are discussed. Methodological problems with existing studies and national suicide statistics are presented. Proposed theories of black suicide are reviewed. Based on a summary of the characteristics of black suicide attempters reported by the literature, preventive strategies--primary, secondary, and tertiary--are presented. PMID:2204709

Mental Health of African Americans and Caribbean Blacks in the United States: Results From the National Epidemiological Survey on Alcohol and Related Conditions

PubMed Central

Okuda, Mayumi; Oquendo, Maria A.; Lawson, William B.; Wang, Shuai; Thomas, Yonette Felicity; Blanco, Carlos

2013-01-01

Objectives. Previous epidemiological studies have found lower mood, anxiety, and substance use disorder prevalence in Black Americans, in general, compared with White Americans. We estimated the prevalence and persistence of psychiatric disorders in African Americans, Caribbean Blacks, and non-Hispanic Whites. Methods. We drew data from wave 1 (2001–2002) of the National Epidemiological Survey of Alcohol and Related Conditions, a nationally representative sample of US adults, which included 7529 African Americans, 469 Caribbean Blacks, and 24 502 non-Hispanic Whites. Results. Blacks had equal or lower prevalence than Whites of lifetime (adjusted odds ratio [AOR] = 0.6 for African Americans; 0.3 for Caribbean Blacks) and 12-month (AOR = 0.7 for African Americans; 0.4 for Caribbean Blacks) Axis I psychiatric disorders, but higher prevalence of several personality disorders. Among Blacks, Caribbean Blacks had higher prevalence of 12-month psychotic disorders and lower lifetime prevalence of major depressive disorder, alcohol dependence, and drug abuse than African Americans. There were no differences in persistence of disorders between Caribbean Blacks and African Americans. Conclusions. This study yielded new data on prevalence of mental disorders in these groups, which has important implications for clinical work with US Blacks. PMID:23237171

Fenton process combined with coagulation for the treatment of black liquor from bioethanol wastewater

NASA Astrophysics Data System (ADS)

Muryanto, Muryanto; Hanifah, Ummu; Amriani, Feni; Ibadurrahman, Ahmad Faiz; Sari, Ajeng Arum

2017-11-01

High amounts of black liquor are generated from bioethanol production by using oil palm empty fruit bunches. The black liquor is waste from alkaline pretreatment, it contains high amount of an alkaline solution (NaOH). The black liquor wastewater was highly contaminated with organic materials, and quite toxic for aquatic ecosystems if discharged directly into waters. This study aimed to determine ability of Fenton process combined with coagulation to treat black liquor. The addition 5% of polyaluminium chloride (PAC) could decolorized black liquor, degraded lignin, and produced sludge 70.64%, 68.28%, and 2.76 gram, respectively. Decolorization of black liquor was in line with degradation of black liquor because lignin is the main compound in black liquor. SEM images after addition of PAC of 5% indicated fragmentation of structure. Fenton reagent consist of 0.7 M FeSO4+ 3M H2O2 has able to decolorize black liquor, degrade lignin, and produce sludge 51.67% and 25.44%, and 0.44 gram, respectively. It was concluded that black liquor wastewater from bioethanol can be treated by using Fenton process combined with coagulation. However, these methods still need improvement to obtain the higher degradation rate, and coagulation sludge needs further consideration.

Effects of anthocyanin and carotenoid combinations on foliage and immature fruit color of Capsicum annuum L.

PubMed

Lightbourn, Gordon J; Griesbach, Robert J; Novotny, Janet A; Clevidence, Beverly A; Rao, David D; Stommel, John R

2008-01-01

Shades ranging from violet to black pigmentation in pepper (Capsicum annuum L.) are attributed to anthocyanin accumulation. High-performance liquid chromatography and mass spectrometry analysis of violet and black fruit tissue identified a single anthocyanin that was determined to be delphinidin-3-p-coumaroyl-rutinoside-5-glucoside. Leaf tissue of a black-pigmented foliage genotype contained the same anthocyanin found in fruit but at a considerably higher concentration in comparison to violet and black fruit tissue. Fruit chlorophyll concentration was approximately 14-fold higher in black fruit in comparison to violet fruit that contained relatively little chlorophyll. Beta-carotene, lutein, violaxanthin, and neoxanthin carotenoid concentrations in black fruit were also significantly greater in comparison to violet fruit. High concentrations of delphinidin in combination with chlorophyll and accessory carotenoid pigments produced the characteristic black pigmentation observed in fruits and leaves of selected genotypes. Anthocyanins were accumulated in the outer mesocarp of violet and black fruit and in the palisade and mesophyll cells of black leaves. Consistent with chlorophyll content of respective genotypes, chloroplast density was greater in cells of black fruits. Utilizing Capsicum pigment variants, we determine the biochemical factors responsible for violet versus black-pigmented pepper tissue in the context of described pepper color genes.

Genetic dissection of black grain rice by the development of a near isogenic line.

PubMed

Maeda, Hiroaki; Yamaguchi, Takuya; Omoteno, Motoyasu; Takarada, Takeshi; Fujita, Kenji; Murata, Kazumasa; Iyama, Yukihide; Kojima, Yoichiro; Morikawa, Makiko; Ozaki, Hidenobu; Mukaino, Naoyuki; Kidani, Yoshinori; Ebitani, Takeshi

2014-06-01

Rice (Oryza sativa L.) can produce black grains as well as white. In black rice, the pericarp of the grain accumulates anthocyanin, which has antioxidant activity and is beneficial to human health. We developed a black rice introgression line in the genetic background of Oryza sativa L. 'Koshihikari', which is a leading variety in Japan. We used Oryza sativa L. 'Hong Xie Nuo' as the donor parent and backcrossed with 'Koshihikari' four times, resulting in a near isogenic line (NIL) for black grains. A whole genome survey of the introgression line using DNA markers suggested that three regions, on chromosomes 1, 3 and 4 are associated with black pigmentation. The locus on chromosome 3 has not been identified previously. A mapping analysis with 546 F2 plants derived from a cross between the black rice NIL and 'Koshihikari' was evaluated. The results indicated that all three loci are essential for black pigmentation. We named these loci Kala1, Kala3 and Kala4. The black rice NIL was evaluated for eating quality and general agronomic traits. The eating quality was greatly superior to that of 'Okunomurasaki', an existing black rice variety. The isogenicity of the black rice NIL to 'Koshihikari' was very high.

Black Hole Boldly Goes Where No Black Hole Has Gone Before

NASA Astrophysics Data System (ADS)

2007-01-01

Astronomers have found a black hole where few thought they could ever exist, inside a globular star cluster. The finding has broad implications for the dynamics of stars clusters and also for the existence of a still-speculative new class of black holes called 'intermediate-mass' black holes. The discovery is reported in the current issue of Nature. Tom Maccarone of the University of Southampton in England leads an international team on the finding, made primarily with the European Space Agency's XMM-Newton satellite. Globular clusters are dense bundles of thousands to millions of old stars, and many scientists have doubted that black holes could survive in such an exclusive environment. Computer simulations show that a newly formed black hole would first sink towards the centre of the cluster but quickly get gravitationally slingshot out entirely when interacting with the cluster's myriad stars. Credit: ESA/Hubble Artist's impression of globular star cluster The new finding provides the first convincing evidence that some black hole might not only survive but grow and flourish in globular clusters. What has astonished astronomers is how quickly the black hole was found. "We were preparing for a long, systematic search of thousands of globular clusters with the hope of finding just one black hole," said Maccarone. "But bingo, we found one as soon as we started the search. It was only the second globular cluster we looked at." The search continues to find more, Maccarone said, yet only one black hole was needed to resolve the decades-old discussion about black holes and globular clusters. Scientists say there are two main classes of black holes. Supermassive black holes containing the mass of millions to billions of suns are found in the core of most galaxies, including our own. A quasar is one kind of supermassive black hole. Stellar-size black holes contain the mass of about ten suns. These are created from the collapsed core of massive stars. Our galaxy likely contains millions of these black holes. Black holes are, by definition, invisible. But the region around them can flare up periodically when the black hole feeds. As gas falls into a black hole, it will heat to high temperatures and radiate brightly, particularly in X-rays. Maccarone's team found one such stellar-mass black hole by chance feeding in a globular cluster in a galaxy named NGC 4472, about fifty million light-years away in the Virgo Cluster. XMM-Newton is extremely sensitive to variable X-ray sources and can efficiently search across large patches of the sky. The team also used NASA's Chandra X-ray Observatory, which has superb angular resolution to pinpoint the X-ray source's location. This allowed them to match up the position of the X-ray source with optical images to prove that the black hole was indeed in a globular cluster. Globular clusters are some of the oldest structures in the universe, containing stars over 12 thousand million years old. Black holes in a cluster would likely have formed many thousand millions of years ago, which is why astronomers have assumed they would have been kicked out a long time ago. Details in the X-ray light detected by XMM-Newton leave little doubt that this is a black hole - the object is too bright, and varies by too much to be anything else. In fact, the source is 'extra bright', - an Ultraluminous X-ray object, or ULX. ULXs are brighter than the 'Eddington limit' for stellar mass black holes, the brightness level at which the outward force from X-rays is expected balance the powerful gravitational forces from the black hole. Thus it is often suggested that the ULXs might be intermediate mass black holes - black holes of thousands of solar masses, heavier than the 10-solar-mass stellar black holes, and lighter than the million to thousand million solar mass black holes in quasars. These black holes might then be the missing links between the black holes formed in the death throes of massive stars and the ones in the centres of galaxies. It is perhaps possible for a stellar-mass black hole to gain enough mass through merging with other stellar-mass black holes or accreting star gas to stay locked in a cluster. About 100 solar masses would do. Once entrenched, the black hole has the opportunity to merge with other black holes or accrete gas from a local neighbourhood rife with star-stuff. In this way, they could grow into IMBHs. "If a black hole is massive enough, there's a good chance it can survive the pressures of living in a globular cluster, since it will be too heavy to be kicked out," said Arunav Kundu of Michigan State University, a co-author on the Nature report. "That's what is intriguing about this discovery. We may be seeing how a black hole can grow considerably, become more entrenched in the cluster, and then grow some more. "On the other hand," continued Kundu, "there are a variety of ways to make ULXs without requiring intermediate mass black holes. In particular, if the light goes out in a different direction than the one from which the gas comes in, it doesn't put any force on the gas. Also, if the light can be 'focused' towards us by reflecting off the gas in the same way that light from a flashlight bulb bounces off the little mirror in the flashlight, making the object appear brighter than it really is." Ongoing work will help to determine whether this object is a stellar-mass black hole showing an unusual manner of sucking in gas, allowing it to be extra bright, or an IMBH. The team, which also includes Steve Zepf from Michigan State University, and Katherine Rhode from Wesleyan University, has data for thousands of other globular clusters, which they are now analyzing in an effort to determine just how common this phenomenon is. Note for editors The findings appear on line in the 4 January issue of the journal Nature, in the article titled: "A black hole in a globular cluster", by Thomas J. Maccarone, Arunav Kundu, Stephen E. Zepf and Katherine L. Rhode.

Why Not Academia?--The Streamlined Career Choice Process of Black African Women Engineers: A Grounded Theory Study

ERIC Educational Resources Information Center

Mlambo, Yeukai Angela

2017-01-01

Black African women are grossly underrepresented as academic staff in engineering programs at South African universities. The problem is exacerbated at historically White institutions (HWI) where Black women are simply absent as engineering research and teaching staff. The absence of Black African women in the academy occurs despite Black African…

Speech for the National Black Child Development Institute Conference (St. Louis, Missouri, October 25, 1991).

ERIC Educational Resources Information Center

Clay, William L.

This speech by a black congressman from Missouri concerns the context in which general questions about black children must be answered. These questions include: (1) How can service providers, institutions, and government entities implement a vision for the future of black children? and (2) How can organizations that serve black children ensure…

Adaptability of black walnut, black cherry, and Northern red oak to Northern California

Treesearch

Philip M. McDonald

1987-01-01

When planted in sheltered sites in northern California, only 49% of black walnut (Juglans nigra L.) and 58% of black cherry (Prunus serotina Ehrh.) survived for 15 years, and 20% of northern red oak (Quercus rubra L.) survived for 10 years. The black walnut trees averaged 0.6 inches diameter at breast...

"Styled by Their Perceptions": Black Adolescent Girls Interpret Representations of Black Females in Popular Culture

ERIC Educational Resources Information Center

Muhammad, Gholnecsar E.; McArthur, Sherell A.

2015-01-01

Identity formation is a critical process shaping the lives of adolescents and can present distinct challenges for Black adolescent girls who are positioned in society to negotiate ideals of self when presented with false and incomplete images representing Black girlhood. Researchers have found distorted images of Black femininity derived from…

A History of Black Professional Football Players from 1900-1946.

ERIC Educational Resources Information Center

Acanfora, Jerry A.

The history of Black professional football is obscure, although it is known that in the early years Black and White athletes played together on basis of ability. Several Black Americans were members of early professional football teams; however, in the late 1920s, they seemed to disappear from team rosters. Each of the eighteen Black professional…

The Delivery of Mental Health Services to Black Children. Final Report.

ERIC Educational Resources Information Center

Gary, Lawrence E.; And Others

This study was designed to start a data base on black mental health practitioners working with black clients. The sample group consisted of 177 (57 men, 120 women) black school counselors and 36 (5 men, 31 women) psychologists in seven predominantly black, urban public schools. The information gathered covered the following areas: (1) demographics…

The Use of Blacks in Magazine and Television Advertising: 1946 to 1986.

ERIC Educational Resources Information Center

Zinkhan, George M.; And Others

1990-01-01

Combines earlier content analysis studies with original data from five magazines in 1986 to examine the use of Blacks in magazine and television advertising. Finds an increase in the Black presence in advertising. Notes that the Black presence in television commercial sometimes approaches the percentage of Blacks in the population. Notes a less…

50 CFR 648.140 - Black sea bass Annual Catch Limit (ACL).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass Annual Catch Limit (ACL... Management Measures for the Black Sea Bass Fishery § 648.140 Black sea bass Annual Catch Limit (ACL). (a) The Black Sea Bass Monitoring Committee shall recommend to the MAFMC separate ACLs for the commercial and...

50 CFR 648.141 - Black sea bass Annual Catch Target (ACT).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Black sea bass Annual Catch Target (ACT... Management Measures for the Black Sea Bass Fishery § 648.141 Black sea bass Annual Catch Target (ACT). (a) The Black Sea Bass Monitoring Committee shall identify and review the relevant sources of management...

50 CFR 648.147 - Black sea bass minimum fish sizes.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass minimum fish sizes. 648... Measures for the Black Sea Bass Fishery § 648.147 Black sea bass minimum fish sizes. (a) Moratorium (commercially) permitted vessels. The minimum size for black sea bass is 11 inches (27.94 cm) total length for...

50 CFR 648.141 - Black sea bass Annual Catch Target (ACT).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass Annual Catch Target (ACT... Management Measures for the Black Sea Bass Fishery § 648.141 Black sea bass Annual Catch Target (ACT). (a) The Black Sea Bass Monitoring Committee shall identify and review the relevant sources of management...

50 CFR 648.147 - Black sea bass minimum fish sizes.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass minimum fish sizes. 648... Measures for the Black Sea Bass Fishery § 648.147 Black sea bass minimum fish sizes. (a) Moratorium (commercially) permitted vessels. The minimum size for black sea bass is 11 inches (27.94 cm) total length for...

50 CFR 648.141 - Black sea bass Annual Catch Target (ACT).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass Annual Catch Target (ACT... Management Measures for the Black Sea Bass Fishery § 648.141 Black sea bass Annual Catch Target (ACT). (a) The Black Sea Bass Monitoring Committee shall identify and review the relevant sources of management...

50 CFR 648.140 - Black sea bass Annual Catch Limit (ACL).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Black sea bass Annual Catch Limit (ACL... Management Measures for the Black Sea Bass Fishery § 648.140 Black sea bass Annual Catch Limit (ACL). (a) The Black Sea Bass Monitoring Committee shall recommend to the MAFMC separate ACLs for the commercial and...

50 CFR 648.146 - Black sea bass recreational fishing season.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass recreational fishing... Management Measures for the Black Sea Bass Fishery § 648.146 Black sea bass recreational fishing season... possession limit specified in § 648.145(a), may only possess black sea bass from May 19 through September 21...

50 CFR 648.140 - Black sea bass Annual Catch Limit (ACL).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass Annual Catch Limit (ACL... Management Measures for the Black Sea Bass Fishery § 648.140 Black sea bass Annual Catch Limit (ACL). (a) The Black Sea Bass Monitoring Committee shall recommend to the MAFMC separate ACLs for the commercial and...

50 CFR 648.147 - Black sea bass minimum fish sizes.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Black sea bass minimum fish sizes. 648... Measures for the Black Sea Bass Fishery § 648.147 Black sea bass minimum fish sizes. (a) Moratorium (commercially) permitted vessels. The minimum size for black sea bass is 11 inches (27.94 cm) total length for...

50 CFR 648.145 - Black sea bass possession limit.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Black sea bass possession limit. 648.145... Measures for the Black Sea Bass Fishery § 648.145 Black sea bass possession limit. (a) During the recreational fishing season specified at § 648.146, no person shall possess more than 15 black sea bass in, or...

50 CFR 648.145 - Black sea bass possession limit.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Black sea bass possession limit. 648.145... Measures for the Black Sea Bass Fishery § 648.145 Black sea bass possession limit. (a) From January 1 through February 28, no person shall possess more than 15 black sea bass in, or harvested from, the EEZ...

50 CFR 648.145 - Black sea bass possession limit.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Black sea bass possession limit. 648.145... Measures for the Black Sea Bass Fishery § 648.145 Black sea bass possession limit. (a) During the recreational fishing season specified at § 648.146, no person shall possess more than 20 black sea bass in, or...