Sample records for black cumin oil

  1. Protective effects of black cumin (Nigella sativa) oil on TNBS-induced experimental colitis in rats.

    PubMed

    Isik, F; Tunali Akbay, Tugba; Yarat, A; Genc, Z; Pisiriciler, R; Caliskan-Ak, E; Cetinel, S; Altıntas, A; Sener, G

    2011-03-01

    The pathogenesis and treatment of ulcerative colitis remain poorly understood. The aim of the present study is to investigate the effects of black cumin (Nigella sativa) oil on rats with colitis. Experimental colitis was induced with 1 mL trinitrobenzene sulfonic acid (TNBS) in 40% ethanol by intracolonic administration with 8-cm-long cannula under ether anesthesia to rats in colitis group and colitis + black cumin oil group. Rats in the control group were given saline at the same volume by intracolonic administration. Black cumin oil (BCO, Origo "100% natural Black Cumin Seed Oil," Turkey) was given to colitis + black cumin oil group by oral administration during 3 days, 5 min after colitis induction. Saline was given to control and colitis groups at the same volume by oral administration. At the end of the experiment, macroscopic lesions were scored and the degree of oxidant damage was evaluated by colonic total protein, sialic acid, malondialdehyde, and glutathione levels, collagen content, and tissue factor, superoxide dismutase, and myeloperoxidase activities. Tissues were also examined by histological and cytological analysis. Proinflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6], lactate dehydrogenase activity, and triglyceride and cholesterol levels were analyzed in blood samples. We found that black cumin oil decreased the proinflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis. BCO, by preventing inflammatory status in the blood, partly protected colonic tissue against experimental ulcerative colitis.

  2. The influence of pulsed electric fields and microwave pretreatments on some selected physicochemical properties of oil extracted from black cumin seed.

    PubMed

    Bakhshabadi, Hamid; Mirzaei, HabibOllah; Ghodsvali, Alireza; Jafari, Seid Mahdi; Ziaiifar, Aman Mohammad

    2018-01-01

    Application of novel technologies such as microwave and pulsed electric fields (PEF) might increase the speed and efficiency of oil extraction. In the present research, PEF (3.25 kV/cm electric field intensity and 30 pulse number) and microwave (540 W for 180 s) pretreatments were used to study the process of oil extraction from black cumin ( Nigella sativa ) seeds. After applying the selected pretreatments, the oil of seeds was extracted with the use of a screw press and the extraction efficiency, refractive index, oil density, color index, oxidative stability, and chemical components of oil and protein of meal were evaluated. The achieved results expressed that PEF and microwave pretreatments increased the oil extraction efficiency and its oxidative stability. Different pretreatments didn't have any significant influence on the refractive index of black cumin seed oil ( p >.05). When microwave and PEF were used, the oil density showed an enhancement as the following: 1.51% and 0.96%, respectively in comparison with the samples with no pretreatments. Evaluation of the extracted oils, using GC/MS analysis indicated that thymoquinone was the dominant phenolic component in the black cumin oil. Finally, the SEM analysis revealed that microwave and PEF can be useful in the extraction of oil from black cumin seeds since these treatments damaged cell walls and facilitated the oil extraction process.

  3. Short and long term modulation of tissue minerals concentrations following oral administration of black cumin (Nigella sativa L.) seed oil to laboratory rats.

    PubMed

    Basheer, Irum; Qureshi, Irfan Zia

    2018-01-15

    Nigella sativa, or commonly called black cumin is a small herb of family Ranunculaceae is a well-known medicinal plant but its effects on tissue mineral concentrations of animal bodies is unknown. To study the effect of oral administration of fixed oil of black cumin seeds on tissues mineral content using laboratory rats as experimental model. Experimental animals were exposed to two oral doses of seed oil (60 and 120 ml kg -1 body weight). Short- and long term experiments lasted 24 h and 60 days respectively, with three replicates each. Oil extracted from black cumin seeds was subjected to GC-MS to identify chemical components. Following the wet digestion in nitric acid, samples of whole blood and organs of rats were subjected to atomic absorption spectrophotometry for determination of elements concentrations. Data were compared statistically at p < .05. Compared to control, Cr, Mn, Ni, Cu, Zn showed decrease, whereas Co, Na, Mg and K demonstrated increase, but Ca showed both increase and decrease in most of the tissues upon short term exposure to low and high doses of black cumin oil. During long term exposure, Cr, Fe, Mn, Cu exhibited decrease; Co, Na, Mg and Ca concentrations demonstrated an upregulation, whereas Ni and Zn showed increase and decrease in most of the tissues. Comparison of short term with long term experiments at low dose revealed increases in Fe, Zn, Cu, Mg, K and Ca, a decrease in Cr, Mn, Ni and Cu in most tissues, but both increase and decrease in Na. At high dose, an increase occurred in Fe, Ni, Zn, K, Ca, Mg, a decrease in Cr, while both increase and decrease in Cu, Co and Na concentrations. Our study demonstrates that oral administration of black cumin seeds oil to laboratory rats significantly alters tissue trace elements and electrolytes concentrations. The study appears beneficial but indicates modulatory role of black cumin oil as regards mineral metabolism with far reaching implications in health and disease. Copyright © 2017. Published by Elsevier GmbH.

  4. Deodorizing Substance in Black Cumin (Nigella sativa L.) Seed Oil.

    PubMed

    Nakasugi, Toru; Murakawa, Takushi; Shibuya, Koji; Morimoto, Masanori

    2017-08-01

    A deodorizing substance in black cumin (Nigella sativa L.), a spice for curry and vegetable foods in Southwest Asia, was examined. The essential oil prepared from the seeds of this plant exhibited strong deodorizing activity against methyl mercaptan, which is a main factor in oral malodor. After purification with silica gel column chromatography, the active substance in black cumin seed oil was identified as thymoquinone. This monoterpenic quinone functions as the main deodorizing substance in this oil against methyl mercaptan. Metabolite analysis suggested that the deodorizing activity may be generated by the addition of a reactive quinone molecule to methyl mercaptan. In the present study, the menthane-type quinone and phenol derivatives exhibited deodorizing activities via this mechanism.

  5. Composition, In Vitro Antioxidant and Antimicrobial Activities of Essential Oil and Oleoresins Obtained from Black Cumin Seeds (Nigella sativa L.)

    PubMed Central

    Singh, Sunita; Das, S. S.; Singh, G.; Schuff, Carola; de Lampasona, Marina P.; Catalán, César A. N.

    2014-01-01

    Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%), α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as 11.47 ± 0.05, 10.88 ± 0.9, 9.68 ± 0.06, and 8.33 ± 0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition against Fusarium moniliforme in inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria. PMID:24689064

  6. Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.).

    PubMed

    Singh, Sunita; Das, S S; Singh, G; Schuff, Carola; de Lampasona, Marina P; Catalán, César A N

    2014-01-01

    Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%), α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as 11.47 ± 0.05, 10.88 ± 0.9, 9.68 ± 0.06, and 8.33 ± 0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition against Fusarium moniliforme in inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria.

  7. Plant growth-promoting bacteria elevate the nutritional and functional properties of black cumin and flaxseed fixed oil.

    PubMed

    Dimitrijević, Snežana; Pavlović, Marija; Maksimović, Svetolik; Ristić, Mihajlo; Filipović, Vladimir; Antonović, Dušan; Dimitrijević-Branković, Suzana

    2018-03-01

    In order to study the influence of plant growth-promoting bacteria (PGPB) belonging to Streptomyces sp., Paenibacillus sp., and Hymenobacter sp. on fixed oil content of flaxseed and black cumin, 2-year field experiments were conducted. PGPB was applied during seedtime of plants. The extraction of oil from seeds was performed using supercritical CO 2 . The addition of PGPB significantly increases the content of C18:1 (from 16.06 ± 0.03% to 16.97 ± 0.03%) and C18:3 (from 42.97 ± 0.2% to 45.42 ± 0.5%) in flaxseed oil and C18:2 (from 52.68 ± 0.50% to 57.11 ± 0.40%) and C20:2 (from 4.34 ± 0.02% to 4.54 ± 0.03%) in black cumin seed oil. The contents of total polyphenols, flavonoids, and carotenoids, as well as antioxidant activity measured by ferric-reducing ability of plasma assay, were found to be greater in the oil from the seeds of plants treated with the PGPB, compared with the respective non-treated samples. The use of PGPB enhances plant nutritive properties; these represent a great source for obtaining valuable functional food ingredients. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  8. Fatty acids, essential oil, and phenolics modifications of black cumin fruit under NaCl stress conditions.

    PubMed

    Bourgou, Soumaya; Bettaieb, Iness; Saidani, Moufida; Marzouk, Brahim

    2010-12-08

    This research evaluated the effect of saline conditions on fruit yield, fatty acids, and essential oils compositions and phenolics content of black cumin (Nigella sativa). This plant is one of the most commonly found aromatics in the Mediterranean kitchen. Increasing NaCl levels to 60 mM decreased significantly the fruits yield by 58% and the total fatty acids amount by 35%. Fatty acids composition analysis indicated that linoleic acid was the major fatty acid (58.09%) followed by oleic (19.21%) and palmitic (14.77%) acids. Salinity enhanced the linoleic acid percentage but did not affect the unsaturation degree of the fatty acids pool and thus the oil quality. The essential oil yield was 0.39% based on the dry weight and increased to 0.53, 0.56, and 0.72% at 20, 40, and 60 mM NaCl. Salinity results on the modification of the essential oil chemotype from p-cymene in controls to γ-terpinene/p-cymene in salt-stressed plants. The amounts of total phenolics were lower in the treated plants. Salinity decreased mainly the amount of the major class, benzoics acids, by 24, 29, and 44% at 20, 40, and 60 mM NaCl. The results suggest that salt treatment may regulate bioactive compounds production in black cumin fruits, influencing their nutritional and industrial values.

  9. Combined effects of gamma-irradiation and modified atmosphere packaging on quality of some spices.

    PubMed

    Kirkin, Celale; Mitrevski, Blagoj; Gunes, Gurbuz; Marriott, Philip J

    2014-07-01

    Thyme (Thymus vidgaris L.), rosemary (Rosmarinus officinalis L.), black pepper (Piper nigrum L.) and cumin (Cuminum cyminum L.) in ground form were packaged in either air or 100% N2 and γ-irradiated at 3 different irradiation levels (7kGy, 12kGy, 17kGy). Total viable bacterial count, yeast and mould count, colour, essential oil yield and essential oil composition were determined. Microbial load was not detectable after 12kGy irradiation of all samples. Irradiation resulted in significant changes in colour values of rosemary and black pepper. The discolouration of the irradiated black pepper was lower in modified atmosphere packaging (MAP) compared to air packaging. Essential oil yield of irradiated black pepper and cumin was lower in air packaging compared to MAP. Gamma-irradiation generally decreased monoterpenes and increased oxygenated compounds, but the effect was lower in MAP. Overall, spices should be irradiated under an O2-free atmosphere to minimise quality deterioration. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. The efficacy of black cumin seed (Nigella sativa) oil and hypoglycemic drug combination to reduce HbA1c level in patients with metabolic syndrome risk

    NASA Astrophysics Data System (ADS)

    Rachman, P. N. R.; Akrom; Darmawan, E.

    2017-11-01

    Metabolic syndrome is a conditions caused by metabolic abnormalities include central obesity, atherogenic dyslipidemia, hypertension, and insulin resistance. HbA1c examination is required to study the long-term glycemic status and to prevent diabetic complications of metabolic syndrome. The purpose of this study is to determine the efficacy of black cumin seed (Nigella sativa) oil and hypoglycemic drug combination to reduce HbA1c level in patients with metabolic syndrome risk. This research performed using an experimental randomized single - blind controlled trial design. A total of 99 outpatients at the Jetis I Public Health Center, Yogyakarta, Indonesia with metabolic syndrome risk were divided into three groups: The control group received placebo and two treatment groups received black seed oil orally at dose of 1.5 mL/day and 3 mL/day, respectively, for 20 days. The clinical conditions such as blood pressure, pulse rate, BMI, blood glucose serum and HbA1c levels were examined on day 0 and 21. The results obtained were analyzed with one-way ANOVA test. The mean of HbA1c levels of all groups before treatment was higher than the normal values and there was no significant difference in HbA1c value on day 0. Administration of 1.5 and 3 mL/day of black seed oil for 20 days decreased (p<0.05) HbA1c levels. It can be concluded that administration of black cumin seed oil and hypoglycemic drug combination for 20 days in patients at risk of metabolic syndrome may reduce to HbA1c levels.

  11. In silico Analysis for Predicting Fatty Acids of Black Cumin Oil as Inhibitors of P-Glycoprotein.

    PubMed

    Ali, Babar; Jamal, Qazi Mohd Sajid; Mir, Showkat R; Shams, Saiba; Al-Wabel, Naser A; Kamal, Mohammad A

    2015-10-01

    Black cumin oil is obtained from the seeds of Nigella sativa L. which belongs to family Ranunculaceae. The seed oil has been reported to possess antitumor, antioxidant, antibacterial, anti-inflammatory, hypoglycemic, central nervous system depressant, antioxidant, and immunostimulatory activities. These bioactivities have been attributed to the fixed oil, volatile oil, or their components. Seed oil consisted of 15 saturated fatty acids (17%) and 17 unsaturated fatty acids (82.9%). Long chain fatty acids and medium chain fatty acids have been reported to increase oral bioavailability of peptides, antibiotics, and other important therapeutic agents. In earlier studies, permeation enhancement and bioenhancement of drugs has been done with black cumin oil. In order to recognize the mechanism of binding of fatty acids to P-glycoprotein (P-gp), linoleic acid, oleic acid, margaric acid, cis-11, 14-eicosadienoic acid, and stearic acid were selected for in silico studies, which were carried out using AutoDock 4.2, based on the Lamarckian genetic algorithm principle. Template search with BLAST and HHblits has been performed against the SWISS-MODEL template library. The target sequence was searched with BLAST against the primary amino acid sequence of P-gp from Rattus norvegicus. The amount of energy needed by linoleic acid, oleic acid, eicosadienoic acid, margaric acid, and stearic acid to bind with P-gp were found to be - 10.60, -10.48, -9.95, -11.92, and - 10.37 kcal/mol, respectively. The obtained data support that all the selected fatty acids have contributed to inhibit P-gp activity thereby enhances the bioavailability of drugs. This study plays a significant role in finding hot spots in P-gp and may offer the further scope of designing potent and specific inhibitors of P-gp. Generation of 3D structure of fatty acid compounds from Black cumin oil and 3D homology modeling of Rat P glycoprotein as a receptor.Rat P-gp structure quality shows 88.5% residues in favored region obtained by Ramchandran plot analysis.Docking analysis revealed that Some amino acids common for all compounds like Ser221, Pro222, Ile224, Gly225, Ser228, Ala229, Lys233, Tyr302, Tyr309, Ile337, Leu338 and Thr341 in the P-gp and ligands binding patterns.Eicosadeinoic acid has highest binding affinity with P-gp as the amount of energy needed to bind with P-gp was lowest (-11.92 kcal/mol). Abbreviations used: P-gp: P-glycoprotein.

  12. Physical, chemical and sensory properties of brownies substituted with sweet potato flour (Ipomoea batatas L.) with addition of black cumin oil (Nigella sativa L.)

    NASA Astrophysics Data System (ADS)

    Ligarnasari, I. P.; Anam, C.; Sanjaya, A. P.

    2018-01-01

    Effect of addition black cumin oil on the physical (hardness) characteristics, chemical (water, ash, fat, protein, carbohydrate, antioxidant IC50, total phenol and active component) characteristics and sensory (flavor, taste, texture, overall) characteristics of brownies substituted sweet potato flour were investigated. Substituted brownies was added with 0.05%, 0.10%, 0.15%, 0.20% and 0.25% of nigella sativa oil. The result showed that water content, ash, protein, fat, total phenol were increased and carbohydrate, antioxidant IC50 was decreased by the addition of nigella sativa oil. Due to the sensory characteristics, panelist gave the high score for substituted brownies which was added 0.05% nigella sativa oil. The result showed that the best formula of substituted brownies which was added 0.05% of nigella sativa oil had 24.89% water content, 1.19% ash content, 7.54% protein content, 37.79% fat content, 53.06% carbohydrate contain, 1043.6 ppm IC50 antioxidant and 0.22% total phenol. The active component on the brownies using GCMS identification were palmitic acid, oleic acid, lauric acid, theobromine and vitamin E.

  13. Antioxidant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rats during aflatoxicosis.

    PubMed

    Abdel-Wahhab, M A; Aly, S E

    2005-01-01

    Aflatoxins, a group of closely related, extremely toxic mycotoxins produced by Aspergillus flavus and A. parasiticus, can occur as natural contaminants of foods and feeds. Aflatoxins have been shown to be hepatotoxic, carcinogenic, mutagenic and teratogenic to different animal species. Nigella sativa (black cumin) and Syzygium aromaticum (clove) oil are used for the treatment of inflammatory diseases and have antioxidant properties. The aim of this study was to investigate the ability of these volatile oils to scavenge free radicals generated during aflatoxicosis. Sixty male rats were divided into six treatment groups, including a control group, and the groups were treated for 30 days with Nigella sativa and Syzygium aromaticum oils with or without aflatoxin. Blood samples were collected at the end of the experimental period for haematological and biochemical analysis. The results indicated that exposure to aflatoxins resulted in haematological and biochemical changes typical for aflatoxicosis. Treatment with Nigella sativa and Syzygium aromaticum oil of rats fed an aflatoxin-contaminated diet resulted in significant protection against aflatoxicosis. Moreover, Nigella sativa oil was found to be more effective than Syzygium aromaticum oil in restoring the parameters that were altered by aflatoxin in rats. Copyright 2005 John Wiley & Sons, Ltd

  14. Evaluation of Synergistic Antibacterial and Antioxidant Efficacy of Essential Oils of Spices and Herbs in Combination

    PubMed Central

    Bag, Anwesa; Chattopadhyay, Rabi Ranjan

    2015-01-01

    The present study was carried out to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of essential oils of some selected spices and herbs [bay leaf, black pepper, coriander (seed and leaf), cumin, garlic, ginger, mustard, onion and turmeric] in combination. Antibacterial combination effect was evaluated against six important food-borne bacteria (Bacillus cereus, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus, Escherichia coli and Salmonella typhimurium) using microbroth dilution, checkerboard titration and time-kill methods. Antioxidant combination effect was assessed by DPPH free radical scavenging method. Total phenolic content was measured by Folin-Ciocalteu method. Bioactivity –guided fractionation of active essential oils for isolation of bioactive compounds was done using TLC-bioautography assay and chemical characterization (qualitative and quantitative) of bioactive compounds was performed using DART-MS and HPLC analyses. Cytotoxic potential was evaluated by brine shrimp lethality assay as well as MTT assay using human normal colon cell line. Results showed that among the possible combinations tested only coriander/cumin seed oil combination showed synergistic interactions both in antibacterial (FICI : 0.25-0.50) and antioxidant (CI : 0.79) activities. A high positive correlation between total phenolic content and antibacterial activity against most of the studied bacteria (R2 = 0.688 – 0.917) as well as antioxidant capacity (R2 = 0.828) was also observed. TLC-bioautography-guided screening and subsequent combination studies revealed that two compounds corresponding to Rf values 0.35 from coriander seed oil and 0.53 from cumin seed oil exhibited both synergistic antibacterial and antioxidant activities. The bioactive compound corresponding to Rf 0.35 from coriander seed oil was identified as linalool (68.69%) and the bioactive compound corresponding to Rf 0.53 from cumin seed oil was identified as p-coumaric acid (7.14%) by DART-MS and HPLC analyses. The coriander/cumin seed oil combination did not show any cytotoxic effect both in brine shrimp lethality as well as human normal colon cell line assays. The LC50 in brine shrimp lethality assay was found to be 4945.30 μg/ml and IC50 in human normal colon cell line was > 1000 μg/ml. The results provide evidence that coriander/cumin seed oil combination might indeed be used as a potential source of safe and effective natural antimicrobial and antioxidant agents in pharmaceutical and food industries. PMID:26132146

  15. From here to eternity - the secret of Pharaohs: Therapeutic potential of black cumin seeds and beyond

    PubMed Central

    Padhye, Subhash; Banerjee, Sanjeev; Ahmad, Aamir; Mohammad, Ramzi; Sarkar, Fazlul H

    2008-01-01

    Summary Over many centuries humans have been mining the bounties of nature for discovering substances that have been used for the treatment of all human diseases; many such remedies are useful even today as modern day medicine. Emerging evidence also suggests that the search is still continuing for harnessing active compounds from nature in combating human illnesses although pharmaceutical industries are equally active for synthesizing small molecule compounds as novel therapeutics. The lesson learned over many centuries clearly suggests that further sophisticated search for finding compounds from natural resources together with robust characterization and chemical synthesis will lead to the discovery of novel drugs that may have high therapeutic efficacy against all human diseases including cancer. Black cumin seed (Nigella sativa) oil extracts have been used for many centuries for the treatment of many human illnesses, and more recently the active compound found in black seed oil, viz. thymoquinone (TQ) has been tested for its efficacy against several diseases including cancer. However, further research is needed in order to assess the full potential of TQ as a chemopreventive and/or therapeutic agent against cancers. Here, we have summarized what is known regarding the value of black seed oil and its active compound TQ, and how this knowledge will help us to advance further research in this field by creating awareness among scientists and health professionals in order to appreciate the medicinal value of TQ and beyond. PMID:19018291

  16. Evaluation of antioxidant potential of essential oils of some commonly used Indian spices in in vitro models and in food supplements enriched with omega-6 and omega-3 fatty acids.

    PubMed

    Bag, Anwesa; Chattopadhyay, Rabi Ranjan

    2018-01-01

    The aim of this study was to evaluate and compare the antioxidant potential of essential oils of some commonly used Indian spices (black pepper, cinnamon, clove, coriander and cumin) in various in vitro models and in food supplements enriched with omega-6 and omega-3 fatty acids. In vitro antioxidant potential was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging and Fe 2+ ion-chelating methods and lipid oxidation stabilisation potential was evaluated in bulk soybean oil-fish oil mixture and their oil-in-water emulsions using peroxide value (PV), p-anisidine value (p-AV) and total oxidation value as indicators of oxidation. Combination effects using DPPH radical scavenging and Briggs-Rauscher oscillating reaction methods were also evaluated. Test essential oils showed varying degrees of radical scavenging and Fe 2+ ion-chelating efficacy. Clove and coriander oils showed significantly higher (P < 0.05) radical scavenging and Fe 2+ ion-chelating potential over other tested essential oils as well as BHT and ∞-tocopherol. The anti-lipid peroxidative potential of test essential oils was found in the following decreasing order: clove > coriander > BHT > cinnamon > α-tocopherol > cumin > black pepper. Furthermore, clove and coriander oils showed synergistic antioxidant activity in combination both in DPPH radical scavenging and Briggs-Rauscher oscillating reaction methods whereas other possible combinations showed additive effects. Strong radical scavenging and Fe 2+ -chelating as well as anti-lipid peroxidative activities of clove and coriander oils provide evidence that clove and coriander oils may serve as a potential source of natural antioxidants for retarding lipid oxidation of food supplements enriched with omega-6 and omega-3 fatty acids.

  17. Inhibitory effects of spice essential oils on the growth of Bacillus species.

    PubMed

    Ozcan, Mehmet Musa; Sağdiç, Osman; Ozkan, Gülcan

    2006-01-01

    A series of essential oils of 11 Turkish plant spices [black thyme, cumin, fennel (sweet), laurel, marjoram, mint, oregano, pickling herb, sage, savory, and thyme], used in foods mainly for their flavor, aromas, and preservation, in herbal tea, in alternative medicines, and in natural therapies, were screened for antibacterial effects at 1:50, 1:100, 1:250, and 1:500 dilutions by the paper disc diffusion method against six Bacillus species (Bacillus amyloliquefaciens ATCC 3842, Bacillus brevis FMC 3, Bacillus cereus FMC 19, Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, and B. subtilis var. niger ATCC 10). All of the tested essential oils (except for cumin) showed antibacterial activity against one or more of the Bacillus species used in this study. Generally, the essential oils at 1:50 and 1:100 levels were more effective. Only one essential oil (laurel) was not found effective against the tested bacteria. The bacterium most sensitive to all of the spice essential oils was B. amyloliquefaciens ATCC 3842. Based on the results of this study, it is likely that essential oils of some spices may be used as antimicrobial agents to prevent the spoilage of food products.

  18. Growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of L. monocytogenes and C. albicans in apple-carrot juice.

    PubMed

    Irkin, Reyhan; Korukluoglu, Mihriban

    2009-04-01

    Food safety is a fundamental concern of both consumers and the food industry. The increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. Spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. In this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on Listeria monocytogenes AUFE 39237, Escherichia coli ATCC 25922, Salmonella enteritidis ATCC 13076, Proteus mirabilis AUFE 43566, Bacillus cereus AUFE 81154, Saccharomyces uvarum UUFE 16732, Kloeckera apiculata UUFE 10628, Candida albicans ATCC 10231, Candida oleophila UUPP 94365, and Metschnikowia fructicola UUPP 23067 and effects of thyme oil at a concentration of 0.5% on L. monocytogenes and C. albicans in apple-carrot juice during +4 degrees C storage (first to fifth day) were investigated. Strong antibacterial and antifungal activities of some essential oils were found. Thyme, origanum, clove, and orange essential oils were the most inhibitory against bacteria and yeasts. Cumin, tea tree, and mint oils inhibited the yeasts actively. It is concluded that some essential oils could be used as potential biopreservatives capable of controlling foodborne pathogens and food spoilage yeasts.

  19. Cardiovascular benefits of black cumin (Nigella sativa).

    PubMed

    Shabana, Adel; El-Menyar, Ayman; Asim, Mohammad; Al-Azzeh, Hiba; Al Thani, Hassan

    2013-03-01

    Black Cumin (Nigella sativa), which belongs to the botanical family of Ranunculaceae, commonly grows in Eastern Europe, the Middle East, and Western Asia. Its ripe fruit contains tiny black seeds, known as "Al-Habba Al-Sauda" and "Al-Habba Al-Barakah" in Arabic and black seed or black cumin in English. Seeds of Nigella sativa are frequently used in folk medicine in the Middle East and some Asian countries for the promotion of good health and the treatment of many ailments. However, data for the cardiovascular benefits of black cumin are not well-established. We reviewed the literature from 1960 to March 2012 by using the following key words: "Nigella sativa," "black seeds," and "thymoquinone." Herein, we discussed the most relevant articles to find out the role of Nigella sativa in the cardiovascular diseases spectrum especially when there is a paucity of information and need of further studies in human to establish the utility of Nigella sativa in cardiovascular system protection.

  20. Distillation Time as Tool for Improved Antimalarial Activity and Differential Oil Composition of Cumin Seed Oil.

    PubMed

    Zheljazkov, Valtcho D; Gawde, Archana; Cantrell, Charles L; Astatkie, Tess; Schlegel, Vicki

    2015-01-01

    A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given duration of the steam distillation time (DT). Ten DT durations were tested in this study: 5, 7.5, 15, 30, 60, 120, 240, 360, 480, and 600 min. Oil yields increased with an increase in the DT. Maximum oil yield (content, 2.3 g/100 seed), was achieved at 480 min; longer DT did not increase oil yields. The concentrations of the major oil constituents α-pinene (0.14-0.5% concentration range), β-pinene (3.7-10.3% range), γ-cymene (5-7.3% range), γ-terpinene (1.8-7.2% range), cumin aldehyde (50-66% range), α-terpinen-7-al (3.8-16% range), and β-terpinen-7-al (12-20% range) varied as a function of the DT. The concentrations of α-pinene, β-pinene, γ-cymene, γ-terpinene in the oil increased with the increase of the duration of the DT; α-pinene was highest in the oil obtained at 600 min DT, β-pinene and γ-terpinene reached maximum concentrations in the oil at 360 min DT; γ-cymene reached a maximum in the oil at 60 min DT, cumin aldehyde was high in the oils obtained at 5-60 min DT, and low in the oils obtained at 240-600 min DT, α-terpinen-7-al reached maximum in the oils obtained at 480 or 600 min DT, whereas β-terpinen-7-al reached a maximum concentration in the oil at 60 min DT. The yield of individual oil constituents (calculated from the oil yields and the concentration of a given compound at a particular DT) increased and reached a maximum at 480 or 600 min DT. The antimalarial activity of the cumin seed oil obtained during the 0-5 and at 5-7.5 min DT timeframes was twice higher than the antimalarial activity of the oils obtained at the other DT. This study opens the possibility for distinct marketing and utilization for these improved oils. The antioxidant capacity of the oil was highest in the oil obtained at 30 min DT and lowest in the oil from 360 min DT. The Michaelis-Menton and the Power nonlinear regression models developed in this study can be utilized to predict essential oil yield and composition of cumin seed at any given duration of DT and may also be useful to compare previous reports on cumin oil yield and composition. DT can be utilized to obtain cumin seed oil with improved antimalarial activity, improved antioxidant capacity, and with various compositions.

  1. Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats.

    PubMed

    Mousavi, Ghafour

    2015-08-01

    To evaluate the effect of Black cumin (Nigella sativa Linn.) pre-treatment on renal ischemia/reperfusion (I/R) induced injury in the rats. A total of 40 male Wistar rats were randomly allocated into five equal groups including Sham, I/R model and three I/R+ Black cumin (0.5, 1 and 2%)-treated groups. I/R groups' kidneys were subjected to 60 min of global ischemia at 37°C followed by 24 h of reperfusion. At the end of reperfusion period, the rats were euthanized. Superoxide dismutase, catalase and glutathione peroxidase activities as well as reduced glutathione and renal malondialdehyde contents were determined in renal tissues. Kidney function tests and histopathological examination were also performed. High serum creatinine, blood urea nitrogen and uric acid as well as malondialhehyde (MDA) levels, and low antioxidant enzyme activities were observed in I/R rats compared to the sham rats. Pre-treatment with Black cumin for three weeks prior to IR operation improved renal function and reduced I/R induced renal inflammation and oxidative injury. These biochemical observations were supported by histopathological test of kidney sections. Black cumin significantly prevented renal ischemia/reperfusion induced functional and histological injuries.

  2. Spices Mycobiota and Mycotoxins Available in Saudi Arabia and Their Abilities to Inhibit Growth of Some Toxigenic Fungi

    PubMed Central

    2007-01-01

    The prevalence and population density of the mycobiota of 50 samples belonging to 10 kinds of spices (anise, black pepper, red pepper, black cumin, peppermint, cardamom, clove, cumin, ginger and marjoram) which collected from different places in Jeddah Governorate were studied. The natural occurrence of mycotoxins in those samples was also investigated. Fifteen genera and thirty - one species of fungi in addition to one species variety were isolated and identified during this study. The most common genera were Aspergillus, Penicillium and Fusarium. Aflatoxins (12~40 µg/kg) were detected in the extract of 5 samples of each of anise seeds and black pepper fruits; three samples of black cumin seeds and on sample only of each of peppermint and marjoram leaves out of 5 samples tested of each. Sterigmatocystin (15~20 µg/kg) was detected in some samples of red pepper, cumin and marjoram. The inhibitory effects of 10 kinds of powdered spices were tested against 3 toxigenic isolates of fungi (Aspergillus flavus, A. versicolor and Penicillium citrinum). Clove proved to be antimycotic compounds. It inhibited the growth of the tested toxigenic fungi. Black pepper, peppermint, cardamom, cumin and marjoram completely inhibited aflatoxins production, while black pepper and cardamom also completely inhibited sterigmatocystin production. PMID:24015069

  3. Distillation time as tool for improved antimalarial activity and differential oil composition of cumin seed oil

    USDA-ARS?s Scientific Manuscript database

    A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given...

  4. Distillation Time as Tool for Improved Antimalarial Activity and Differential Oil Composition of Cumin Seed Oil

    PubMed Central

    Zheljazkov, Valtcho D.; Gawde, Archana; Cantrell, Charles L.; Astatkie, Tess; Schlegel, Vicki

    2015-01-01

    A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given duration of the steam distillation time (DT). Ten DT durations were tested in this study: 5, 7.5, 15, 30, 60, 120, 240, 360, 480, and 600 min. Oil yields increased with an increase in the DT. Maximum oil yield (content, 2.3 g/100 seed), was achieved at 480 min; longer DT did not increase oil yields. The concentrations of the major oil constituents α-pinene (0.14–0.5% concentration range), β-pinene (3.7–10.3% range), γ-cymene (5–7.3% range), γ-terpinene (1.8–7.2% range), cumin aldehyde (50–66% range), α-terpinen-7-al (3.8–16% range), and β-terpinen-7-al (12–20% range) varied as a function of the DT. The concentrations of α-pinene, β-pinene, γ-cymene, γ-terpinene in the oil increased with the increase of the duration of the DT; α-pinene was highest in the oil obtained at 600 min DT, β-pinene and γ-terpinene reached maximum concentrations in the oil at 360 min DT; γ-cymene reached a maximum in the oil at 60 min DT, cumin aldehyde was high in the oils obtained at 5–60 min DT, and low in the oils obtained at 240–600 min DT, α-terpinen-7-al reached maximum in the oils obtained at 480 or 600 min DT, whereas β-terpinen-7-al reached a maximum concentration in the oil at 60 min DT. The yield of individual oil constituents (calculated from the oil yields and the concentration of a given compound at a particular DT) increased and reached a maximum at 480 or 600 min DT. The antimalarial activity of the cumin seed oil obtained during the 0–5 and at 5–7.5 min DT timeframes was twice higher than the antimalarial activity of the oils obtained at the other DT. This study opens the possibility for distinct marketing and utilization for these improved oils. The antioxidant capacity of the oil was highest in the oil obtained at 30 min DT and lowest in the oil from 360 min DT. The Michaelis-Menton and the Power nonlinear regression models developed in this study can be utilized to predict essential oil yield and composition of cumin seed at any given duration of DT and may also be useful to compare previous reports on cumin oil yield and composition. DT can be utilized to obtain cumin seed oil with improved antimalarial activity, improved antioxidant capacity, and with various compositions. PMID:26641276

  5. Evaluation of the protective effect of Nigella sativa extract and its primary active component thymoquinone against DMBA-induced breast cancer in female rats

    PubMed Central

    Linjawi, Sabah A. A.; Hassanane, Mahrosa M.; Ahmed, Ekram S.

    2013-01-01

    Introduction The historical use of black cumin seed (Nigella sativa) dates back centuries, being embedded in Arabian culture and having a long history of unsurpassed medicinal value with versatility to treat a wide range of ailments. Thymoquinone (TQ) is now known to be the primary active constituent of black cumin seed oil (BCS oil) responsible for its medicinal effects and also showing promise for treatment of cancer. Material and methods In the current study, we have studied the effects of TQ and BCS oil on tumor markers (MDA, LDH, ALP and AST), histopathological alterations and the regulation of several genes (Brca1, Brca2, Id-1 and P53 mutation) related to breast cancer in female rats induced by 7,12-dimethylbenz[a]anthracene (DMBA) treatment. Rats received a single dose (65 mg/kg b.w.) of DMBA via an intragastric tube to induce breast cancer. Animals that received DMBA were treated orally with 1, 5, 10 mg/kg of TQ or BCS oil via an intragastric tube three times per week for 4 months. Results We found that TQ and then BCS reduced the rate of tumor markers (levels of MDA and LDH as well as ALP and AST activities), inhibited the histopathological alterations and decreased the expression of the Brca1, Brca2, Id-1 and P53 mutations in mammary tissues of female rats induced by DMBA treatment. Conclusions The results suggest that TQ and BCS oil exert a protective effect against breast carcinogens. The antioxidant property of TQ and BCS oil is mediated by their actions and investigating other underlying mechanisms merits further studies. PMID:25861310

  6. Supplementation of Nigella sativa fixed and essential oil mediates potassium bromate induced oxidative stress and multiple organ toxicity.

    PubMed

    Sultan, Muhammad Tauseef; Butt, Masood Sadiq; Ahmad, Rabia Shabeer; Pasha, Imran; Ahmad, Atif Nisar; Qayyum, Mir Muhammad Nasir

    2012-01-01

    The plants and their functional ingredients hold potential to cure various maladies and number of plants hold therapeutic potential. The present research was designed study the health promoting potential of black cumin (Nigella sativa) fixed oil (BCFO) and essential oil (BCEO) against oxidative stress with special reference to multiple organ toxicity. For the purpose, thirty rats (Strain: Sprague Dawley) were procured and divided into three groups (10 rats/group). The groups were fed on their respective diets i.e. D1 (control), D2 (BCFO @ 4.0%) and D3 (BCEO @ 0.30%) for a period of 56 days. Mild oxidative stress was induced with the help of potassium bromate injection @ 45 mg/Kg body weight. Furthermore, the levels of cardiac and liver enzymes were assayed. The results indicated that oxidative stress increased the activities of cardiac and liver enzymes. However, supplementation of BCFO and BCEO was effective in reducing the abnormal values of enzymes. Elevated levels of lactate dehydrogenase (LDH), CPK and CPK-MB were reduced from 456 to 231, 176 to 122 and 45 to 36mg/dL, respectively. Similarly, liver enzymes were also reduced. However, the results revealed that BCEO supplementation @ 0.30% is more effectual in ameliorating the multiple organ toxicity in oxidative stressed animal modelling. In the nutshell, it can be assumed that black cumin essential oil is more effective in reducing the extent of potassium bromate induced multiple organ toxicity (cardiac and liver enzymes imbalance) that will ultimately helpful in reducing the extent of myocardial and liver necrosis.

  7. Protective effect of treatment with black cumin oil on spatial cognitive functions of rats that suffered global cerebrovascular hypoperfusion.

    PubMed

    Azzubaidi, Marwan Saad; Saxena, Anil Kumar; Talib, Norlelawati Abi; Ahmed, Qamar Uddin; Dogarai, Bashar Bello

    2012-01-01

    The fixed oil of black cumin seeds, Nigella sativa L. (NSO), has shown considerable antioxidant and anti-inflammatory activities. Chronic cerebral hypoperfusion has been linked to neurodegenerative disorders including Alzheimer's disease (AD) and its subsequent cognitive impairment in which oxidative stress and neuroinflammation are the principal culprits. Cerebrovascular hypoperfusion was experimentally achieved by bilateral common carotid arteries occlusion (2VO) in rats. Morris water maze (MWM) test was employed to assess the effects of NSO on spatial cognitive function before and after 2VO intervention. Rats were divided into long-term memory (LTM) and short-term memory (STM) groups, each was further subdivided into 3 subgroups: sham control, untreated 2VO and NSO treated 2VO group. All subgroups were tested with MWM at the tenth postoperative week. Working memory test results for both sham control and NSO treated groups showed significantly lower escape latency time and total distance travelled than untreated 2VO group. Similarly, LTM and STM MWM tests for sham control and NSO treated groups revealed significantly better maze test performance as compared to untreated 2VO group. Sham control and NSO treated 2VO groups demonstrated superior probe memory test performance as compared to untreated 2VO group. The fixed oil of Nigella sativa seeds has demonstrated noticeable spatial cognitive preservation in rats challenged with chronic cerebral hypoperfusion which indicates a promising prospective neuroprotective effect.

  8. Effect of cumin essential oil usage on fermentation quality, aerobic stability and in vitro digetibility of alfalfa silage.

    PubMed

    Turan, Aslı; Önenç, Sibel Soycan

    2018-03-02

    This study was carried out to determine the effects of cumin essential oil on the silage fermentation, aerobic stability and in vitro digestibility of alfalfa silages. Alfalfa was harvested at early bloom (5 th cutting) stage in October and wilted for about 3 hours. The research was carried out at three groups which were the control group where no additive control was done (CON), cumin essential oil (CMN3) with 300 mg/kg and CMN5 with 500 mg/kg cumin essential oil addition. Alfalfa was ensiled in plastic bags. The packages were stored at 8±2 °C under laboratory conditions. All groups were sampled for physical, chemical and microbiological analysis 120th day after ensiling. At the end of the ensiling period, all silages were subjected to an aerobic stability test for 7 days. In addition, enzimatic solubility of organic matter (ESOM), metabolizable energy (ME) and relative feed value (RFV) of these silages were determined. pH level decreased in the cumin groups compared to CON (P<0.05), thus inhibiting proteolytic enzymes from breaking down proteins into ammonia. In addition, it increased ESOM amount, and concordantly provided an increase of ME contents. Similarly, dry matter intake (DMI) and RFV ratio increased. After opening the silage, it kept its aerobic stability for three days. Cumin essential oil improved fermentation, and affected chemical and microbiological characteristics of silages. Especially the addition of 300 mg/kg cumin provided cell wall fractionation through stimulating the activities of enzymes responsible. It also increased the number and activity of lactic acid bacteri (LAB) through providing a development of LAB.

  9. Corrosion Mechanism of Low-Carbon Steel in Industrial Water and Adsorption Thermodynamics in the Presence of Some Plant Extracts

    NASA Astrophysics Data System (ADS)

    Badiea, A. M.; Mohana, K. N.

    2009-12-01

    The effects of radish leaves and black cumin as plant extracts on the corrosion behavior of low-carbon steel in industrial water in the temperature range of 30 to 80 °C and velocity range of 1.44 to 2.02 m s-1 using potentiodynamic polarization, electrochemical impedance spectroscopy, and mass loss measurements have been investigated. The inhibition efficiency increased with increasing concentration of the plant extracts up to a critical value but it slightly decreased with increasing temperature. Inhibition efficiency values obtained from mass loss and potentiodynamic data were in reasonable agreement. Potentiodynamic polarization clearly indicated that radish leaves and black cumin extracts acted as anodic inhibitors. The adsorption behavior was found to obey the Flory-Huggins isotherm model. The associated activation parameters and thermodynamic data of adsorption were evaluated and discussed. The results show that radish leaves and black cumin could serve as effective inhibitors for low-carbon steel in industrial water media, with black cumin providing better protection than radish leaves.

  10. Processes to Preserve Spice and Herb Quality and Sensory Integrity During Pathogen Inactivation

    PubMed Central

    Moberg, Kayla; Amin, Kemia N.; Wright, Melissa; Newkirk, Jordan J.; Ponder, Monica A.; Acuff, Gary R.; Dickson, James S.

    2017-01-01

    Abstract Selected processing methods, demonstrated to be effective at reducing Salmonella, were assessed to determine if spice and herb quality was affected. Black peppercorn, cumin seed, oregano, and onion powder were irradiated to a target dose of 8 kGy. Two additional processes were examined for whole black peppercorns and cumin seeds: ethylene oxide (EtO) fumigation and vacuum assisted‐steam (82.22 °C, 7.5 psia). Treated and untreated spices/herbs were compared (visual, odor) using sensory similarity testing protocols (α = 0.20; β = 0.05; proportion of discriminators: 20%) to determine if processing altered sensory quality. Analytical assessment of quality (color, water activity, and volatile chemistry) was completed. Irradiation did not alter visual or odor sensory quality of black peppercorn, cumin seed, or oregano but created differences in onion powder, which was lighter (higher L *) and more red (higher a*) in color, and resulted in nearly complete loss of measured volatile compounds. EtO processing did not create detectable odor or appearance differences in black peppercorn; however visual and odor sensory quality differences, supported by changes in color (higher b *; lower L *) and increased concentrations of most volatiles, were detected for cumin seeds. Steam processing of black peppercorn resulted in perceptible odor differences, supported by increased concentration of monoterpene volatiles and loss of all sesquiterpenes; only visual differences were noted for cumin seed. An important step in process validation is the verification that no effect is detectable from a sensory perspective. PMID:28407236

  11. Isolation and identification of bacteria causing mastitis in small ruminants and their susceptibility to antibiotics, honey, essential oils, and plant extracts

    PubMed Central

    Abdalhamed, Abeer Mostafa; Zeedan, Gamil Sayed Gamil; Zeina, Hala Abdoula Ahmed Abou

    2018-01-01

    Aim: The present work aims to isolate and identify bacteria that cause mastitis in small ruminants and evaluates the antibacterial activity of some antibiotics, honey, essential oils, and plant extracts. Materials and Methods: A total of 289 milk samples were collected from udder secretions of sheep (n=189) and goat (n=100) from El-Fayoum, Beni-Suef, and Giza governorates. Screening subclinical mastitis (SCM) was done using California Mastitis Test (CMT); identification of the isolates was achieved using Gram’s staining, hemolytic pattern, colony morphology, and biochemical tests using Analytical Profile Index. Results: On clinical examination, the incidence of clinical mastitis (CM) was found to be 5.88% and 7% in sheep and goat, respectively. On CMT, SCM was found to be 25 (13.23%) and 11 (10%) in sheep and goat, respectively. Bacteriological examination of all milk samples found the presence of Staphylococcus aureus (SA) (31.1%), coagulase-negative staphylococci (CNS) (19.5%), Escherichia coli (EC) (8.3%), Streptococcus spp. (5.6%), Klebsiella spp. (3.77%), and Pseudomonas spp. (1.89%), while no bacteria were cultured from 81.66% of the samples. Identification of 9 isolates of CNS was achieved by using API staph test to Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus cohnii, and Staphylococcus saprophyticus. The highest bacterial resistance was found in EC (67.14%) followed by Kp (45.28%) and SA (26.57%). Conclusion: Onion and black cumin essential oils followed by Egyptian honey showed strong antibacterial effects against multidrug-resistant bacteria. Finally, our study proved that Egyptian honey, onion, and black cumin essential oils have a marked strong antibacterial effect against bacteria isolated from small ruminant mastitis, but still further extensive studies are needed to discover the therapeutic properties of these plant extracts and honey. PMID:29657429

  12. Black Cumin (Nigella sativa) and Its Active Constituent, Thymoquinone: An Overview on the Analgesic and Anti-inflammatory Effects.

    PubMed

    Amin, Bahareh; Hosseinzadeh, Hossein

    2016-01-01

    For many centuries, seeds of Nigella sativa (black cumin), a dicotyledon of the Ranunculaceae family, have been used as a seasoning spice and food additive in the Middle East and Mediterranean areas. Traditionally, the plant is used for asthma, hypertension, diabetes, inflammation, cough, bronchitis, headache, eczema, fever, dizziness, and gastrointestinal disturbances. The literature regarding the biological activities of seeds of this plant is extensive, citing bronchodilative, anti-inflammatory, antinociceptive, antibacterial, hypotensive, hypolipidemic, cytotoxic, antidiabetic, and hepatoprotective effects. The active ingredients of N. sativa are mainly concentrated in the fixed or essential oil of seeds, which are responsible for most health benefits. This review will provide all updated reported activities of this plant with an emphasis on the antinociceptive and anti-inflammatory effects. Results of various studies have demonstrated that the oil, extracts, and their active ingredients, in particular, thymoquinone, possess antinociceptive and anti-inflammatory effects, supporting the common folk perception of N. Sativa as a potent analgesic and anti-inflammatory agent. Many protective properties are attributed to reproducible radical scavenging activity as well as an interaction with numerous molecular targets involved in inflammation, including proinflammatory enzymes and cytokines. However, there is a need for further investigations to find out the precise mechanisms responsible for the antinociceptive and anti-inflammatory effects of this plant and its active constituents. Georg Thieme Verlag KG Stuttgart · New York.

  13. Processes to Preserve Spice and Herb Quality and Sensory Integrity During Pathogen Inactivation.

    PubMed

    Duncan, Susan E; Moberg, Kayla; Amin, Kemia N; Wright, Melissa; Newkirk, Jordan J; Ponder, Monica A; Acuff, Gary R; Dickson, James S

    2017-05-01

    Selected processing methods, demonstrated to be effective at reducing Salmonella, were assessed to determine if spice and herb quality was affected. Black peppercorn, cumin seed, oregano, and onion powder were irradiated to a target dose of 8 kGy. Two additional processes were examined for whole black peppercorns and cumin seeds: ethylene oxide (EtO) fumigation and vacuum assisted-steam (82.22 °C, 7.5 psia). Treated and untreated spices/herbs were compared (visual, odor) using sensory similarity testing protocols (α = 0.20; β = 0.05; proportion of discriminators: 20%) to determine if processing altered sensory quality. Analytical assessment of quality (color, water activity, and volatile chemistry) was completed. Irradiation did not alter visual or odor sensory quality of black peppercorn, cumin seed, or oregano but created differences in onion powder, which was lighter (higher L * ) and more red (higher a * ) in color, and resulted in nearly complete loss of measured volatile compounds. EtO processing did not create detectable odor or appearance differences in black peppercorn; however visual and odor sensory quality differences, supported by changes in color (higher b * ; lower L * ) and increased concentrations of most volatiles, were detected for cumin seeds. Steam processing of black peppercorn resulted in perceptible odor differences, supported by increased concentration of monoterpene volatiles and loss of all sesquiterpenes; only visual differences were noted for cumin seed. An important step in process validation is the verification that no effect is detectable from a sensory perspective. © 2017 The Authors. Journal of Food Science published by Wiley Periodicals, Inc. on behalf of Institute of Food Technologists.

  14. Characterization of butter spoiling yeasts and their inhibition by some spices.

    PubMed

    Sagdic, Osman; Ozturk, Ismet; Bayram, Okan; Kesmen, Zulal; Yilmaz, Mustafa Tahsin

    2010-01-01

    This study was designed to identify the yeasts in packaged and unpackaged butters and screen antiyeast activity of spices, including marjoram (Origanum majorana L.), summer savory (Satureja hortensis L.), and black cumin (Nigella sativa L.) against the most dominant yeast species in the packaged and unpackaged butters. Mean total yeast populations were 5.40 log CFU/g in unpackaged butter samples and 2.22 log CFU/g in packaged butter samples, indicating better hygienic quality of packaged samples. Forty-nine yeast species were isolated and identified from butter samples with the most prevalent isolates belonging to genera Candida-C. kefyr, C. zeylanoides, and C. lambica-and with moderate number of isolates belonging to genera Cryptococcus, Rhodotorula, Saccharomyces, and Zygosaccharomyces. Black cumin exhibited the highest antiyeast activity against C. zeylanoides and C. lambica species, even inhibited these species, while summer savory inhibited C. kefyr. The results of this study revealed clear antimicrobial potential of black cumin against the yeast species isolated from butters. Marjoram, summer savory, and black cumin could be used as natural antimicrobial agents against spoilage yeasts in food preservation, especially in butter. © 2010 Institute of Food Technologists®

  15. Therapeutic role of Cuminum cyminum on ethanol and thermally oxidized sunflower oil induced toxicity.

    PubMed

    Aruna, K; Rukkumani, R; Varma, P Suresh; Menon, Venugopal P

    2005-05-01

    Ethanol is one of the most widely used and abused drugs, increasing lipid levels in humans and experimental animals. Heating of oil rich in polyunsaturated fatty acids (PUFA) produces various lipid peroxidative end products that can aggravate the pathological changes produced by ethanol. In the present communication, the effect of Cuminum cyminum was investigated on alcohol and thermally oxidized oil induced hyperlipidaemia. The results showed increased activity of aspartate transaminase (AST), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) and increased levels of cholesterol, triglycerides and phospholipids in the plasma of rats given alcohol, thermally oxidized oil and alcohol+thermally oxidized oil when compared with the normal control group. The levels of tissue (liver and kidney) cholesterol and triglycerides were increased significantly in rats groups given alcohol, thermally oxidized oil and alcohol+thermally oxidized oil when compared with the normal control rats. The levels were decreased when cumin was given along with alcohol and thermally oxidized oil. The level of phospholipids decreased significantly in the liver and kidney of groups given alcohol, thermally oxidized oil and alcohol+thermally oridized oil when compared with the normal control rats. The level increased when cumin was administered along with alcohol and thermally oxidized oil. The activity of phospholipase A and C increased significantly in the liver of groups given alcohol, thermally oxidized oil and alcohol+thermally oxidized oil when compared with the normal control rats, whereas the activity was decreased with the cumin treatment. The results obtained indicate that cumin can decrease the lipid levels in alcohol and thermally oxidized oil induced hepatotoxicity. Copyright (c) 2005 John Wiley & Sons, Ltd.

  16. A Comparison Between the Effect of Cuminum Cyminum and Vitamin E on the Level of Leptin, Paraoxonase 1, HbA1c and Oxidized LDL in Diabetic Patients

    PubMed Central

    Samani Keihan, Ghatreh; Gharib, Mohammad Hossein; Momeni, Ali; Hemati, Zohreh; Sedighin, Roya

    2016-01-01

    Diabetes is one of the most common metabolic diseases in the world. Vitamin E reduces protein glycation and improves insulin sensitivity, while cumin is effective in remission of diabetes. Therefore this study was designed to evaluate the effects of vitamin E and cumin essential oil, on the blood level of leptin,glycosylated hemoglobin (HbA1C) and also on lipid profile in diabetic patients.In this double blind clinical trial, 95 diabetic patients were selected and randomly dividedinto three groups.The first group received cumin essential oil in capsule form. The second group received Vitamin E, and the third group was used ascontrol receiving oral gelatin capsules as placebo for three months period.Blood glucose, lipid profile, apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), leptin, HbA1c, oxidized LDL (oxLDL), and paraoxonase1 activity were measured. The results showed reduction in oxLDL and significant increase in paraoxonase 1 in Vitamin E group by the end of the third month period (P<0.05). Cumin group showed decrease in blood glucose, HbA1C, triglyceride, leptin and ox-LDL. ApoA1 and paraoxonase1 were also increased by cumin treatment (P<0.05).Diabetic complications may have been reduced by intake of Vitamin E and cumin essential oil. Cumin in comparison with vitamin E has broader impact and it is more beneficial in terms of ability to reduce the diabetic index. PMID:28357199

  17. Inhibition of growth and aflatoxin production in Aspergillus parasiticus by essential oils of selected plant materials.

    PubMed

    Tantaoui-Elaraki, A; Beraoud, L

    1994-01-01

    We studied the effect of 13 chemically different essential oils (EO) on the mycelial growth of and aflatoxin synthesis by Aspergillus parasiticus. Cinnamon, thyme, oregano, and cumin EO were able to stop mycelial growth at only 0.1% in the medium, while curcumin, ginger, lemon, and orange EO were unable to inhibit totally the growth even at 1% concentration. Coriander, black pepper, mugwort, bay, and rosemary EO caused the growth to stop at concentrations between 0.2 and 1%. The EO most active upon mycelial growth were also the most active against aflatoxinogenesis. However, aflatoxin synthesis was inhibited by all the EO at higher extent than the mycelial growth.

  18. The influence of black cumin seed oil therapy with dosage of 1.5 mL/day and 3 mL/day to interleukin-21 (IL-21) expression of the patients with metabolic syndrome risk

    NASA Astrophysics Data System (ADS)

    Fajar, D. R.; Akrom; Darmawan, E.

    2017-11-01

    Metabolic syndrome (MS) is a metabolic disorder caused by obesity and insulin resistance. In the state of obesity and insulin resistance occurs increased fat metabolism that causes production reactive oxygen species (ROS) and oxidative stress that makes dysregulation of adipose tissue that decreases antioxidant enzymes and immune system disorders. In diabetes mellitus there is pancreatic β cell damage caused by pro-inflammatory cytokines i.e interleukin-21 (IL-21). Black cumin seed oil (BCSO) contains antioxidants and immunomodulators but has not seen how it affects to IL-21. This study used cross over design to determine the effect of BCSO with dose of 1.5 mL/day and 3 mL/day dose of IL-21 in MS patients. The total subjects 66 of MS patients were divided into 2 groups. Group I was administered 1.5 mL/day and 3 mL/day of BCSO in dosage for 20 days and continued with washing out (BCSO discontinued) for 7 days. On the day 28, group I administrated 3 mL/day of BCSO and group II were given 1.5 mL/day of BCSO during 20 days. Statistical analysis showed that mean value expression of IL-21 were given by BCSO at dose 1.5 mL/day was 5.06±5.48 and BCSO at dose 3 mL/day 4.66±3.63 (p<0.05). The result showed that IL-21 expression in patient at risk of MS who received BCSO dose 3 mL/day were lower than those received adose of 1.5 mL/day for 20 days.

  19. Acaricidal effect and chemical composition of essential oils extracted from Cuminum cyminum, Pimenta dioica and Ocimum basilicum against the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

    PubMed

    Martinez-Velazquez, Moises; Castillo-Herrera, Gustavo Adolfo; Rosario-Cruz, Rodrigo; Flores-Fernandez, Jose Miguel; Lopez-Ramirez, Julisa; Hernandez-Gutierrez, Rodolfo; Lugo-Cervantes, Eugenia del Carmen

    2011-02-01

    Acaricidal activity of essential oils extracted from cumin seeds (Cuminum cyminum), allspice berries (Pimenta dioica) and basil leaves (Ocimum basilicum) were tested on 10-day-old Rhipicephalus (Boophilus) microplus tick larvae using the LPT. Two-fold dilutions of the three essential oils were tested from a starting dilution of 20% down to 1.25%. Results showed a high toxicological effect for cumin, producing 100% mortality in all tested concentrations on R. microplus larvae. Similarly, allspice essential oil produced 100% mortality at all concentrations with the exception of a dramatic decrease at 1.25% concentration. Conversely, basil essential oil was not shown to be toxic against R. microplus larvae. The most common compounds detected by gas chromatography-mass spectrometry were as follows: cumin: cuminaldehyde (22.03%), γ-terpinene (15.69%) and 2-caren-10-al (12.89%); allspice: methyl eugenol (62.7%) and eugenol (8.3%); basil: linalool (30.61%) and estragole (20.04%). Results clearly indicate that C. cyminum and P. dioica essential oils can be used as an effective alternative for R. microplus tick control, and there is a high probability they can be used for other ticks affecting cattle in Mexico and throughout the world, thereby reducing the necessity for traditional and unfriendly synthetic acaricides.

  20. Inhibitory effects of some plant essential oils against Arcobacter butzleri and potential for rosemary oil as a natural food preservative.

    PubMed

    Irkin, Reyhan; Abay, Secil; Aydin, Fuat

    2011-03-01

    We investigated the inhibitory activity of commercially marketed essential oils of mint, rosemary, orange, sage, cinnamon, bay, clove, and cumin against Arcobacter butzleri and Arcobacter skirrowii and the effects of the essential oil of rosemary against A. butzleri in a cooked minced beef system. Using the disc diffusion method to determine the inhibitory activities of these plant essential oils against strains of Arcobacter, we found that those of rosemary, bay, cinnamon, and clove had strong inhibitory activity against these organisms, whereas the essential oils of cumin, mint, and sage failed to show inhibitory activity against most of the Arcobacter strains tested. The 0.5% (vol/wt) essential oil of rosemary was completely inhibitory against A. butzleri in the cooked minced beef system at 4°C. These essential oils may be further investigated as a natural solution to the food industry by creating an additional barrier (hurdle technology) to inhibit the growth of Arcobacter strains.

  1. Anticancer activities of Nigella sativa (black cumin).

    PubMed

    Khan, Md Asaduzzaman; Chen, Han-chun; Tania, Mousumi; Zhang, Dian-zheng

    2011-01-01

    Nigella sativa has been used as traditional medicine for centuries. The crude oil and thymoquinone (TQ) extracted from its seeds and oil are effective against many diseases like cancer, cardiovascular complications, diabetes, asthma, kidney disease etc. It is effective against cancer in blood system, lung, kidney, liver, prostate, breast, cervix, skin with much safety. The molecular mechanisms behind its anticancer role is still not clearly understood, however, some studies showed that TQ has antioxidant role and improves body's defense system, induces apoptosis and controls Akt pathway. Although the anti-cancer activity of N. sativa components was recognized thousands of years ago but proper scientific research with this important traditional medicine is a history of last 2∼3 decades. There are not so many research works done with this important traditional medicine and very few reports exist in the scientific database. In this article, we have summarized the actions of TQ and crude oil of N. sativa against different cancers with their molecular mechanisms.

  2. Pharmaceutical Perspectives of Spices and Condiments as Alternative Antimicrobial Remedy

    PubMed Central

    D’Souza, Savita P.; Chavannavar, Suvarna V.; Kanchanashri, B.; Niveditha, S. B.

    2017-01-01

    Medicinal values of spices and condiments are being revived by biologists through in vitro and in vivo trials providing evidence for its antimicrobial activities. The essential oils and extracts of spices like black pepper, cloves, cinnamon, and nutmeg contain active compounds like piperine, eugenol, cinnamaldehyde, and lignans. Similarly, condiments like coriander, black cumin, turmeric, garlic, and ginger are recognized for constituents like linalool, thymoquinones, curcumin, allicin, and geranial respectively. These act as natural preventive components of several diseases and represent as antioxidants in body cells. Scientists have to investigate the biochemical nature, mode of action, and minimum concentration of administrating active ingredients effectively. This review reports findings of recent research carried out across South Asia and Middle East countries where spices and condiments form chief flavoring components of traditional foods. It narrates the history, myths, and facts people believe in these regions. There may not be scientific explanation but has evidence of cure for centuries. PMID:28449595

  3. Therapeutic Potential and Pharmaceutical Development of Thymoquinone: A Multitargeted Molecule of Natural Origin.

    PubMed

    Goyal, Sameer N; Prajapati, Chaitali P; Gore, Prashant R; Patil, Chandragouda R; Mahajan, Umesh B; Sharma, Charu; Talla, Sandhya P; Ojha, Shreesh K

    2017-01-01

    Thymoquinone, a monoterpene molecule is chemically known as 2-methyl-5-isopropyl-1, 4-benzoquinone. It is abundantly present in seeds of Nigella sativa L. that is popularly known as black cumin or black seed and belongs to the family Ranunculaceae . A large number of studies have revealed that thymoquinone is the major active constituent in N. sativa oil this constituent is responsible for the majority of the pharmacological properties. The beneficial organoprotective activities of thymoquinone in experimental animal models of different human diseases are attributed to the potent anti-oxidant and anti-inflammatory properties. Thymoquinone has also been shown to alter numerous molecular and signaling pathways in many inflammatory and degenerative diseases including cancer. Thymoquinone has been reported to possess potent lipophilicity and limited bioavailability and exhibits light and heat sensitivity. Altogether, these physiochemical properties encumber the successful formulation for the delivery of drug in oral dosages form and restrict the pharmaceutical development. In recent past, many efforts were undertaken to improve the bioavailability for clinical usage by manipulating the physiochemical parameters. The present review aimed to provide insights regarding the physicochemical characteristics, pharmacokinetics and the methods to promote pharmaceutical development and endorse the clinical usage of TQ in future by overcoming the associated physiochemical obstacles. It also enumerates briefly the pharmacological and molecular targets of thymoquinone as well as the pharmacological properties in various diseases and the underlying molecular mechanism. Though, a convincing number of experimental studies are available but human studies are not available with thymoquinone despite of the long history of use of black cumin in different diseases. Thus, the clinical studies including pharmacokinetic studies and regulatory toxicity studies are required to encourage the clinical development of thymoquinone.

  4. Therapeutic Potential and Pharmaceutical Development of Thymoquinone: A Multitargeted Molecule of Natural Origin

    PubMed Central

    Goyal, Sameer N.; Prajapati, Chaitali P.; Gore, Prashant R.; Patil, Chandragouda R.; Mahajan, Umesh B.; Sharma, Charu; Talla, Sandhya P.; Ojha, Shreesh K.

    2017-01-01

    Thymoquinone, a monoterpene molecule is chemically known as 2-methyl-5-isopropyl-1, 4-benzoquinone. It is abundantly present in seeds of Nigella sativa L. that is popularly known as black cumin or black seed and belongs to the family Ranunculaceae. A large number of studies have revealed that thymoquinone is the major active constituent in N. sativa oil this constituent is responsible for the majority of the pharmacological properties. The beneficial organoprotective activities of thymoquinone in experimental animal models of different human diseases are attributed to the potent anti-oxidant and anti-inflammatory properties. Thymoquinone has also been shown to alter numerous molecular and signaling pathways in many inflammatory and degenerative diseases including cancer. Thymoquinone has been reported to possess potent lipophilicity and limited bioavailability and exhibits light and heat sensitivity. Altogether, these physiochemical properties encumber the successful formulation for the delivery of drug in oral dosages form and restrict the pharmaceutical development. In recent past, many efforts were undertaken to improve the bioavailability for clinical usage by manipulating the physiochemical parameters. The present review aimed to provide insights regarding the physicochemical characteristics, pharmacokinetics and the methods to promote pharmaceutical development and endorse the clinical usage of TQ in future by overcoming the associated physiochemical obstacles. It also enumerates briefly the pharmacological and molecular targets of thymoquinone as well as the pharmacological properties in various diseases and the underlying molecular mechanism. Though, a convincing number of experimental studies are available but human studies are not available with thymoquinone despite of the long history of use of black cumin in different diseases. Thus, the clinical studies including pharmacokinetic studies and regulatory toxicity studies are required to encourage the clinical development of thymoquinone. PMID:28983249

  5. Distribution of primary and specialized metabolites in Nigella sativa seeds, a spice with vast traditional and historical uses.

    PubMed

    Botnick, Ilan; Xue, Wentao; Bar, Einat; Ibdah, Mwafaq; Schwartz, Amnon; Joel, Daniel M; Lev, Efraim; Fait, Aaron; Lewinsohn, Efraim

    2012-08-24

    Black cumin (Nigella sativa L., Ranunculaceae) is an annual herb commonly used in the Middle East, India and nowadays gaining worldwide acceptance. Historical and traditional uses are extensively documented in ancient texts and historical documents. Black cumin seeds and oil are commonly used as a traditional tonic and remedy for many ailments as well as in confectionery and bakery. Little is known however about the mechanisms that allow the accumulation and localization of its active components in the seed. Chemical and anatomical evidence indicates the presence of active compounds in seed coats. Seed volatiles consist largely of olefinic and oxygenated monoterpenes, mainly p-cymene, thymohydroquinone, thymoquinone, γ-terpinene and α-thujene, with lower levels of sesquiterpenes, mainly longifolene. Monoterpene composition changes during seed maturation. γ-Terpinene and α-thujene are the major monoterpenes accumulated in immature seeds, and the former is gradually replaced by p-cymene, carvacrol, thymo-hydroquinone and thymoquinone upon seed development. These compounds, as well as the indazole alkaloids nigellidine and nigellicine, are almost exclusively accumulated in the seed coat. In contrast, organic and amino acids are primarily accumulated in the inner seed tissues. Sugars and sugar alcohols, as well as the amino alkaloid dopamine and the saponin α-hederin accumulate both in the seed coats and the inner seed tissues at different ratios. Chemical analyses shed light to the ample traditional and historical uses of this plant.

  6. Effect of cryogenic grinding on volatile and fatty oil constituents of cumin (Cuminum cyminum L.) genotypes.

    PubMed

    Sharma, L K; Agarwal, D; Rathore, S S; Malhotra, S K; Saxena, S N

    2016-06-01

    Effect of cryogenic grinding on recovery of volatile oil, fatty oil percentage and their constituents in two cumin (Cuminum cyminum L.) genotypes have been analyzed. Cryogenic grinding not only retains the volatiles but enhanced the recovery by 33.9 % in GC 4 and 43.5 % in RZ 209. A significant increase (29.9 %) over normal grinding in oil percentage was also observed in genotype RZ 209. This increase was, however, less (15.4 %) in genotype GC 4. Nineteen major compounds were identified in the essential oil of both genotypes. The two grinding techniques had significant effects on dependent variables, viz., volatile oil and monoterpenes. Cuminaldehyde was the main constituent in both genotypes, content of which increased from 48.2 to 56.1 % in GC 4 on cryo grinding. Content of terpines were found to decrease in cryo ground samples of GC 4 and either decrease or no change was found in RZ 209. Organoleptic test showed more pleasant aroma in cryo ground seeds of both the genotypes. Significant increase was also reported in fatty oil yield due to cryogenic grinding. Fatty acid methyl ester (FAME) analysis showed oleic acid as major FAME content of which increased from 88.1 to 94.9 % in RZ 209 and from 88.2 to 90.1 % in GC 4 on cryogenic grinding. Other prominent FAME were palmitic, palmitoleic and stearic acid. Results indicated commercial potential of cryogenic grinding technology for cumin in general and spices in particular for better retention of flavour and quality in spices.

  7. Cuminum cyminum and Carum carvi: An update

    PubMed Central

    Johri, R. K.

    2011-01-01

    Cuminum cyminum and Carum carvi are the sources of cumin and caraway seeds respectively, which have been used since antiquity for the treatment of various indications in traditional healing systems in wide geographical areas. Cumin and caraway seeds are rich sources of essential oils and have been actively researched for their chemical composition and biological activities. In recent times (especially during the last 3 years) considerable progress has been made regarding validation of their acclaimed medicinal attributes by extensive experimental studies. In this attempt many novel bioactivities have been revealed. This review highlights the significance of cumin and caraway as potential source of diverse natural products and their medicinal applications. PMID:22096320

  8. Anti-rancidity effect of essential oils, application in the lipid stability of cooked turkey meat patties and potential implications for health.

    PubMed

    Loizzo, Monica R; Tundis, Rosa; Menichini, Francesco; Duthie, Garry

    2015-02-01

    Twenty-three commercial essential oils were tested for their anti-rancidity effect and potential implications to prolong the induction time of corn oil and extend the shelf life of cooked turkey patties. Moreover, the potential health benefit was investigated through DPPH, ABTS, β-carotene bleaching, FRAP, and α-amylase inhibitory assays. Essential oils' composition was investigated by GC-MS. Cumin, thyme, clove, and cinnamon oils improved oxidative stability and increased the induction time of the corn oil 1.5-3 fold. Clove and cinnamon oils were particularly effective in delaying lipid oxidation of cooked turkey patties (time of induction 11.04 and 9.43 h) compared with the plain burger (5.04 h). Both oils are also characterized by a potent radical scavenging activity in ABTS test (IC(50) values of 1.43 and 2.05 μg/ml for cinnamon and clove, respectively). In the α-amylase inhibitory assay, cumin and grape fruits were the most potent with IC(50) values of 21.88 and 23.95 μg/ml, respectively.

  9. Antifungal compounds from turmeric and nutmeg with activity against plant pathogens

    USDA-ARS?s Scientific Manuscript database

    The antifungal activity of twenty-two common spices was evaluated against plant pathogens using direct-bioautography coupled Colletotrichum bioassays. Turmeric, nutmeg, ginger, clove, oregano, cinnamon, anise, fennel, basil, black cumin, and black pepper showed antifungal activity against the plant ...

  10. Differentiation of cumin seeds using a metal-oxide based gas sensor array in tandem with chemometric tools.

    PubMed

    Ghasemi-Varnamkhasti, Mahdi; Amiri, Zahra Safari; Tohidi, Mojtaba; Dowlati, Majid; Mohtasebi, Seyed Saeid; Silva, Adenilton C; Fernandes, David D S; Araujo, Mário C U

    2018-01-01

    Cumin is a plant of the Apiaceae family (umbelliferae) which has been used since ancient times as a medicinal plant and as a spice. The difference in the percentage of aromatic compounds in cumin obtained from different locations has led to differentiation of some species of cumin from other species. The quality and price of cumin vary according to the specie and may be an incentive for the adulteration of high value samples with low quality cultivars. An electronic nose simulates the human olfactory sense by using an array of sensors to distinguish complex smells. This makes it an alternative for the identification and classification of cumin species. The data, however, may have a complex structure, difficult to interpret. Given this, chemometric tools can be used to manipulate data with two-dimensional structure (sensor responses in time) obtained by using electronic nose sensors. In this study, an electronic nose based on eight metal oxide semiconductor sensors (MOS) and 2D-LDA (two-dimensional linear discriminant analysis), U-PLS-DA (Partial least square discriminant analysis applied to the unfolded data) and PARAFAC-LDA (Parallel factor analysis with linear discriminant analysis) algorithms were used in order to identify and classify different varieties of both cultivated and wild black caraway and cumin. The proposed methodology presented a correct classification rate of 87.1% for PARAFAC-LDA and 100% for 2D-LDA and U-PLS-DA, indicating a promising strategy for the classification different varieties of cumin, caraway and other seeds. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Relation between salt tolerance and biochemical changes in cumin (Cuminum cyminum L.) seeds.

    PubMed

    Bettaieb Rebey, Iness; Bourgou, Soumaya; Rahali, Fatma Zohra; Msaada, Kamel; Ksouri, Riadh; Marzouk, Brahim

    2017-04-01

    In this study, the effects of salinity on growth, fatty acid, essential oil, and phenolic composition of cumin (Cuminum cyminum L.) seeds as well as the antioxidant activities of their extracts were investigated. Plants were treated with different concentrations of NaCl treatment: 0, 50, 75, and 125 mmoL. Plant growth was significantly reduced with the severity of saline treatment. This also caused important reductions in the seed yield and yield components. Besides, NaCl treatments affected fatty acid composition. Petroselinic and linoleic acids proportions diminished consistently with the increase in NaCl concentration, whereas palmitic acid proportion increased. Furthermore, NaCl enhanced essential oil production in C. cyminum seeds and induced marked changes on the essential oil quality. Essential oil chemotype was modified from γ-terpinene/1-phenyl-1,2 ethanediol in control to γ-terpinene/β-pinene in salt stressed plants. Total polyphenol content was higher in treated seeds, and salinity improved the amount of individual phenolic compounds. Moreover, antioxidant activities of the extracts were determined by four different test systems, namely 2,2-diphenyl-1-picrylhydrazyl, β-carotene/linoleic acid chelating, and reducing power assays. The highest antioxidant activities were reveled in severe stressed plants. In this case, cumin seeds produced under saline conditions may function as a potential source of essential oil and antioxidant compounds, which could support the utilization of this plant in a large field of applications such as food industry. Copyright © 2016. Published by Elsevier B.V.

  12. 21 CFR 182.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf...

  13. 21 CFR 182.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf...

  14. 21 CFR 182.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf...

  15. 21 CFR 582.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican oregano, Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum...

  16. 21 CFR 582.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican oregano, Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum...

  17. 21 CFR 582.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican oregano, Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum...

  18. 21 CFR 582.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican oregano, Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum...

  19. 21 CFR 182.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf...

  20. 21 CFR 582.10 - Spices and other natural seasonings and flavorings.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... Capsicum Capsicum frutescens L. or Capsicum annuum L. Caraway Carum carvi L. Caraway, black (black cumin... pepper Capsicum frutescens L. or Capsicum annuum L. Celery seed Apium graveolens L. Chervil Anthriscus..., Mexican oregano, Mexican sage, origan) Lippia spp. Paprika Capsicum annuum L. Parsley Petroselinum crispum...

  1. Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.

    PubMed

    Edris, Amr E; Kalemba, Danuta; Adamiec, Janusz; Piątkowski, Marcin

    2016-08-01

    Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature. The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying. The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated. The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis. Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder. On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7. The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Physicochemical properties of water-soluble polysaccharides from black cumin seeds.

    PubMed

    Trigui, Ines; Yaich, Héla; Sila, Assaâd; Cheikh-Rouhou, Salma; Bougatef, Ali; Blecker, Christophe; Attia, Hamadi; Ayadi, M A

    2018-06-01

    In the present work, water-soluble polysaccharides were isolated from black cumin seeds. Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction. The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated. BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%). BCWSP was composed of high peak molecular weight. The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm -1 , which corresponded to COO - of uronic acids and pyranose form, respectively. NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains. X-ray pattern revealed the semi-crystalline behavior of BCWSP. WHC and OHC of BCWSP were relatively high and varied with temperatures. The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities. The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL. The BCWSP displayed moderate reductive, β carotene bleaching and chelating abilities. Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products. Copyright © 2017. Published by Elsevier B.V.

  3. Effect of ultraviolet and far infrared radiation on microbial decontamination and quality of cumin seeds.

    PubMed

    Erdoğdu, S Belgin; Ekiz, H İbrahim

    2011-01-01

    Cumin seeds might be exposed to a high level of natural bacterial contamination, and this could potentially create a public health risk besides leading to problems in exportation. Ultraviolet (UVC) and far infrared (FIR) radiation has low penetration power, and due to that, there might be no detrimental defects to the products during a possible decontamination process. Therefore, the objective of this study was to determine the effect of UVC and FIR treatment on microbial decontamination and quality of cumin seeds. For this purpose, FIR treatment at different exposure times and temperatures were applied followed by constant UVC treatment with an intensity of 10.5 mW/cm² for 2 h. Total mesophilic aerobic bacteria of the cumin seeds were decreased to the target level of 10⁴ CFU/g after 1.57, 2.8, and 4.8 min FIR treatment at 300, 250, and 200 °C, respectively, following a 2 h UVC treatment. Under the given conditions, a complete elimination for total yeast and molds were obtained while there were no significant changes in volatile oil content and color of the cumin seeds. Consequently, combined UVC and FIR treatment was determined to be a promising method for decontamination of the cumin seeds. This research attempts to apply UVC and far infrared (FIR) radiation for pasteurization of cumin seeds. The data suggested that combined UVC and FIR radiation treatments can become a promising new method for pasteurization of cumin seeds without causing any detrimental defect to the quality parameters. The results of this industry partnered (Kadioglu Baharat, Mersin, Turkey--http://www.kadioglubaharat.com) study were already applied in industrial scale production lines. © 2011 Institute of Food Technologists®

  4. Cometabolic degradation of trichloroethene by Rhodococcus sp. strain L4 immobilized on plant materials rich in essential oils.

    PubMed

    Suttinun, Oramas; Müller, Rudolf; Luepromchai, Ekawan

    2010-07-01

    The cometabolic degradation of trichloroethene (TCE) by Rhodococcus sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively, Rhodococcus sp. L4 was immobilized on plant materials which contain those inducers in their essential oils. Cumin seeds were the most suitable immobilizing material, and the immobilized cells tolerated up to 68 muM TCE and degraded TCE continuously. The activity of immobilized cells, which had been inactivated partially during TCE degradation, could be reactivated by incubation in mineral salts medium without TCE. These findings demonstrate that immobilization of Rhodococcus sp. L4 on plant materials rich in essential oils is a promising method for efficient cometabolic degradation of TCE.

  5. Cumin Extract for Symptom Control in Patients with Irritable Bowel Syndrome: A Case Series

    PubMed Central

    Agah, Shahram; Taleb, Amir Mehdi; Moeini, Reyhane; Gorji, Narjes; Nikbakht, Hajar

    2013-01-01

    BACKGROUND Irritable bowel syndrome is one of the most common gastrointestinal disorders Characterized by chronic abdominal pain, altered bowel habits or changes in stool consistency. Unfortunately, no specific treatments for relieving IBS symptoms have been suggested yet. This pilot study was conducted to evaluate the efficacy of the Cumin extract, a kind of herbal used in the treatment of gastrointestinal disorders like bloating, and other symptoms of IBS. METHODS Fifty seven patients with IBS (according to the ROME II diagnostic criteria) with no nay other accompanying illness enrolled in study. Patients were advised to discontinue their other treatments during the study course, then 20 drops per day of Cumin essential oil was administered for included patients. IBS-associated symptoms including abdominal pain, nausea, painful defection, presence of mucosa in stool, changes in stool consistency and defecation frequency were evaluated using a questionnairebefore treatment, 2 and 4 weeks after beginning treatment and 2 and 4 weeks after stopping treatment. RESULTS Abdominal pain, bloating, incomplete defecation, fecal urgency and presence of mucus discharge in stool were statistically significant decreased during and after treatment with Cumin extract. Stool consistency and defecation frequency were also both statistically significant improved in patients with constipation dominant pattern of IBS. CONCLUSION Cumin extract can be effective in improving all IBS symptoms. Considering its low cost and easy availability Cumin administration in patients with IBS may have economic benefits. PMID:24829694

  6. Comparative effects of using black seed (Nigella sativa), cumin seed (Cuminum cyminum), probiotic or prebiotic on growth performance, blood haematology and serum biochemistry of broiler chicks.

    PubMed

    Alimohamadi, K; Taherpour, K; Ghasemi, H A; Fatahnia, F

    2014-06-01

    A 42-day trial was conducted to compare the effects of the following seven experimental diets, which varied in black seed, cumin seed, probiotic or prebiotic concentrations, on the broiler chicks: control (no additives), diet BS1 (4 g/kg black seed), diet BS2 (8 g/kg black seed), diet CS1 (4 g/kg cumin seed), diet CS2 (8 g/kg cumin seed), diet Pro (1 g/kg probiotic Primalac(®)) and diet Pre (2 g/kg prebiotic Fermacto(®)). A total of 420 1-day-old male broiler chicks, initially weighing an average of 43 g, were distributed into 28 floor pens at a stocking density of 15 birds per pen. At 28 day of age, the body weight in the birds fed diets BS2, CS2 and Pro was significantly higher than in the control group, but final body weight was not affected. Additionally, the birds fed diets BS2, Pro and Pre exhibited better feed conversion ratio than control birds from 0 to 42 day of age. Diets BS2, CS2 and Pro also statistically increased the relative weight of thymus and bursa of Fabricius, whereas only diet Pro decreased the abdominal fat percentage compared with control diet. Regarding the haematological parameters, feeding diet BS2 yielded a significant increase in red blood cell count, haemoglobin concentration and haematocrit percentage compared with control diet. Serum total cholesterol and low-density lipoprotein cholesterol levels in the birds fed diets BS2, Pro and Pre were also significantly lower than in the birds fed the control diet. Without exception, no diets affected feed intake, internal organs weights, carcass characteristics, antibody titres against Newcastle and influenza viruses and leucocyte subsets. In general, current study showed promising results regarding the use of spice additives as growth and health promoters, especially at higher levels of their incorporation in the diets, which were comparable to the probiotic- or prebiotic-containing diets. Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.

  7. Antifungal and antiaflatoxigenic properties of Cuminum cyminum (L.) seed essential oil and its efficacy as a preservative in stored commodities.

    PubMed

    Kedia, Akash; Prakash, Bhanu; Mishra, Prashant K; Dubey, N K

    2014-01-03

    The study reports potential of Cuminum cyminum (cumin) seed essential oil (EO) as a plant based shelf life enhancer against fungal and aflatoxin contamination and lipid peroxidation. The EO showed efficacy as a preservative in food systems (stored wheat and chickpeas). A total of 1230 fungal isolates were obtained from food samples, with Aspergillus flavus LHP(C)-D6 identified as the highest aflatoxin producer. Cumin seed EO was chemically characterized through GC-MS where cymene (47.08%) was found as the major component. The minimum inhibitory concentration and minimum aflatoxin inhibitory concentration of EO were 0.6 and 0.5 μl/ml respectively. The EO showed toxicity against a broad spectrum of food borne fungi. The antifungal action of EO on ergosterol content in the plasma membrane of A. flavus was determined. The EO showed strong antioxidant potential having IC50 0.092 μl/ml. As a fumigant in food systems, the EO provided sufficient protection of food samples against fungal association without affecting seed germination. In view of the antifungal and antiaflatoxigenic nature, free radical scavenging potential and efficacy in food system, cumin seed EO may be able to provide protection of food commodities against quantitative and qualitative losses, thereby enhancing their shelf life. The present investigation comprises the first report on antifungal mode of action of cumin seed EO and its efficacy as fumigant in food systems. © 2013.

  8. Mycobiota and Natural Incidence of Aflatoxins, Ochratoxin A, and Citrinin in Indian Spices Confirmed by LC-MS/MS

    PubMed Central

    Jeswal, Punam; Kumar, Dhiraj

    2015-01-01

    Nine different Indian spices (red chilli, black pepper, turmeric, coriander, cumin, fennel, caraway, fenugreek, and dry ginger) commonly cultivated and highly used in India were analysed for natural occurrence of toxigenic mycoflora and aflatoxins (AFs), ochratoxin A (OTA), and citrinin (CTN) contamination. Aspergillus flavus and Aspergillus niger were the most dominant species isolated from all types of spices. Red chilli samples were highly contaminated with aflatoxins (85.4%) followed by dry ginger (77.7%). 56% Aspergillus flavus from red chilli and 45% Aspergillus ochraceus from black pepper were toxigenic and produced aflatoxins and ochratoxin A, respectively. Qualitative detection and quantitative detection of mycotoxins in spices were analyzed by ELISA and further confirmed by LC-MS/MS. Penicillium citrinum produced citrinin in red chilli, black pepper, coriander, cumin, fenugreek, and dry ginger samples. The highest amount of AFs was found in red chilli (219.6 ng/g), OTA was in black pepper (154.1 ng/g), and CTN was in dry ginger samples (85.1 ng/g). The results of this study suggest that the spices are susceptible substrate for growth of mycotoxigenic fungi and further mycotoxin production. This is the first report of natural occurrence of citrinin in black pepper and dry ginger from India. PMID:26229535

  9. Cometabolic Degradation of Trichloroethene by Rhodococcus sp. Strain L4 Immobilized on Plant Materials Rich in Essential Oils▿ †

    PubMed Central

    Suttinun, Oramas; Müller, Rudolf; Luepromchai, Ekawan

    2010-01-01

    The cometabolic degradation of trichloroethene (TCE) by Rhodococcus sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively, Rhodococcus sp. L4 was immobilized on plant materials which contain those inducers in their essential oils. Cumin seeds were the most suitable immobilizing material, and the immobilized cells tolerated up to 68 μM TCE and degraded TCE continuously. The activity of immobilized cells, which had been inactivated partially during TCE degradation, could be reactivated by incubation in mineral salts medium without TCE. These findings demonstrate that immobilization of Rhodococcus sp. L4 on plant materials rich in essential oils is a promising method for efficient cometabolic degradation of TCE. PMID:20472723

  10. Chemical composition and biological activities of Tunisian Cuminum cyminum L. essential oil: a high effectiveness against Vibrio spp. strains.

    PubMed

    Hajlaoui, Hafedh; Mighri, Hedi; Noumi, Emira; Snoussi, Mejdi; Trabelsi, Najla; Ksouri, Riadh; Bakhrouf, Amina

    2010-01-01

    Essential oil extracted by hydrodistillation from Tunisian variety of Cuminumcyminum was characterized by means of GC and GC-MS. Twenty-one components were identified and C. cyminum contained cuminlaldehyde (39.48%), gamma-terpinene (15.21%), O-cymene (11.82%), beta-pinene (11.13%), 2-caren-10-al (7.93%), trans-carveol (4.49%) and myrtenal (3.5%) as a major components. Moreover, C. cyminum oil exhibited higher antibacterial and antifungal activities with a high effectiveness against Vibrio spp. strains with a diameter of inhibition zones growth ranging from 11 to 23 mm and MIC and MBC values ranging from (0.078-0.31 mg/ml) to (0.31-1.25mg/ml), respectively. On the other hand, the cumin oil was investigated for its antioxidant activities using four different tests then compared with BHT. Results showed that cumin oil exhibit a higher activity in each antioxidant system with a special attention for beta-carotene bleaching test (IC(50): 20 microg/ml) and reducing power (EC(50): 11 microg/ml). In the light of these findings, we suggested that C. cyminum essential oil may be considered as an interesting source of antibacterial, antifungal and antioxidants components used as potent agents in food preservation and for therapeutic or nutraceutical industries. Copyright (c) 2010. Published by Elsevier Ltd.

  11. Some Properties of Fresh and Ripened Traditional Akcakatik Cheese

    PubMed Central

    2018-01-01

    Akcakatik cheese (yogurt cheese) is produced by drying strained yogurt with or without adding cloves or black cumin. The main objective of this study was to detect the properties of both fresh and ripened Akcakatik cheeses and to compare them. For this purpose the biogenic amine content, volatile flavor compounds, protein degradation level, chemical properties and some microbiological properties of 15 Akcakatik cheese samples were investigated. Titratable acidity, total dry matter, NaCl, total nitrogen, water soluble nitrogen, ripened index, histamine, diacetyl and acetaldehyde levels were found to be higher in ripened cheese samples than in fresh cheese samples. On the other hand, the clove and black cumin ratios were found to be higher in the fresh cheese samples. Sodium dodecyl sulphate polyacrylamide gel electropherograms of cheese samples showed that protein degradation was higher in ripened cheese samples than in fresh samples, as expected. The dominant Lactic acid bacteria (LAB) flora of Akcakatik cheese samples were found to be Streptococcus salivarius subsp. thermophilus and Lactobacillus delbrueckii subsp. bulgaricus. PMID:29725229

  12. In vitro antibacterial activity of seven Indian spices against high level gentamicin resistant strains of enterococci

    PubMed Central

    Bipin, Chapagain; Chitra, Pai (Bhat); Minakshi, Bhattacharjee

    2015-01-01

    Introduction The aim of the study was to explore the in vitro antibacterial activity of seven ethanolic extracts of spices against high level gentamicin resistant (HLGR) enterococci isolated from human clinical samples. Material and methods Two hundred and fifteen enterococcal strains were isolated from clinical samples. High level gentamicin resistance in ethanolic extracts of cumin (Cuminum cyminum), cinnamon (Cinnamomum zeylanicum), ginger (Zingiber officinale), fenugreek (Trigonella foenum-graecum), cloves (Syzygium aromaticum), cardamom (Elettaria cardamomum Maton) and black pepper (Piper nigrum) were prepared using Soxhlet apparatus. The antibacterial effect of the extracts was studied using the well diffusion method. Statistical analysis was carried out by χ2 test using SPSS 17 software. Results Only cinnamon and ginger were found to have activity against all the isolates, whereas cumin and cloves had a variable effect on the strains. Fenugreek, black pepper and cardamom did not show any effect on the isolates. The zone diameter of inhibition obtained for cinnamon, ginger, cloves and cumin was in the range 31–34 mm, 27–30 mm, 25–26 mm and 19–20 mm respectively. Conclusions Cinnamomum zeylanicum and Z. officinale showed the maximum antibacterial activity against the enterococcal isolates followed by S. aromaticum and C. cyminum. The findings of the study show that spices used in the study can contribute to the development of potential antimicrobial agents for inclusion in the anti-enterococcal treatment regimen. PMID:26322099

  13. Insecticidal and acetylcholine esterase inhibition activity of Apiaceae plant essential oils and their constituents against adults of German cockroach (Blattella germanica).

    PubMed

    Yeom, Hwa-Jeong; Kang, Jae Soon; Kim, Gil-Hah; Park, Il-Kwon

    2012-07-25

    We evaluated the insecticidal and acetylcholine esterase (AChE) inhibition activity of 11 Apiaceae plant essential oils and their constituents in adult male and female Blattella germanica. Of the 11 Apiaceae plant essential oils tested, dill (Anethum graveolens), carvi (Carum carvi), and cumin (Cuminum cyminum) demonstrated >90% fumigant toxicity against adult male German cockroaches at a concentration of 5 mg/filter paper. In a contact toxicity test, dill (Anethum graveolens), carvi (Carum carvi), cumin (Cuminum cyminum), and ajowan (Trachyspermum ammi) produced strong insecticidal activity against adult male and female German cockroaches. Among the test compounds, (S)-(+)-carvone, 1,8-cineole, trans-dihydrocarvone, cuminaldehyde, trans-anethole, p-cymene, and γ-terpinene demonstrated strong fumigant toxicity against adult male and female B. germanica. In a contact toxicity test, carveol, cuminaldehyde, (S)-(+)-carvone, trans-anethole, thymol, and p-cymene showed strong contact toxicity against adult male and female B. germanica. IC(50) values of α-pinene, carvacrol, and dihydrocarvone against female AChE were 0.28, 0.17, and 0.78 mg/mL, respectively. The toxicity of the blends of constituents identified in 4 active oils indicated that carvone, cuminaldehyde, and thymol were major contributors to the fumigant activity or contact toxicity of the artificial blend.

  14. A Social History of the Tenth Cavalry, 1931-1941

    DTIC Science & Technology

    1976-06-11

    Moton’s assessment of the situation and sympathized with his efforts to change conditions of service for the black regiments. MacArthur stressed the...Carter, Ernest, 2909 Lockridge, Kansas City, KS 64128 Carter, George B., 601 Yum, Manhattan, KS 66502 Cumins , Charles, 2860 Ivanhoe, Denver, CO 80207

  15. Larvicidal and acetylcholinesterase inhibitory activities of apiaceae plant essential oils and their constituents against aedes albopictus and formulation development.

    PubMed

    Seo, Seon-Mi; Jung, Chan-Sik; Kang, Jaesoon; Lee, Hyo-Rim; Kim, Sung-Woong; Hyun, Jinho; Park, Il-Kwon

    2015-11-18

    This study evaluated the larvicidal activity of 12 Apiaceae plant essential oils and their components against the Asian tiger mosquito, Aedes albopictus, and the inhibition of acetylcholine esterase with their components. Of the 12 plant essential oils tested, ajowan (Trachyspermum ammi), caraway seed (Carum carvi), carrot seed (Daucus carota), celery (Apium graveolens), cumin (Cuminum cyminum), dill (Anethum graveolens), and parsley (Petroselinum sativum) resulted in >90% larval mortality when used at 0.1 mg/mL. Of the compounds identified, α-phellandrene, α-terpinene, p-cymene, (-)-limonene, (+)-limonene, γ-terpinene, cuminaldehyde, neral, (S)-+-carvone, trans-anethole, thymol, carvacrol, myristicin, apiol, and carotol resulted in >80% larval mortality when used at 0.1 mg/mL. Two days after treatment, 24.69, 3.64, and 12.43% of the original amounts of the celery, cumin, and parsley oils, respectively, remained in the water. Less than 50% of the original amounts of α-phellandrene, 1,8-cineole, terpinen-4-ol, cuminaldehyde, and trans-antheole were detected in the water at 2 days after treatment. Carvacrol, α-pinene, and β-pinene inhibited the activity of Ae. albopictus acetylcholinesterase with IC50 values of 0.057, 0.062, and 0.190 mg/mL, respectively. A spherical microemulsion of parsley essential oil-loaded poly(vinyl alcohol) (PVA) was prepared, and the larvicidal activity of this formulation was shown to be similar to that of parsley oil.

  16. Inhibitory effect of gamma radiation and Nigella sativa seeds oil on growth, spore germination and toxin production of fungi

    NASA Astrophysics Data System (ADS)

    Zeinab, E. M. EL-Bazza; Hala, A. Farrag; Mohie, E. D. Z. EL-Fouly; Seham, Y. M. EL-Tablawy

    2001-02-01

    Twenty samples of Nigella sativa seeds (Black cumin) were purchased from different localities in Egypt. The mold viable count ranged from 1.7×10 1 to 9.8×10 3 c.f.u. Sixty six molds were isolated belonging to six genera Aspergillus, Penicillium, Rhizopus, Mucor, Alternaria and Fusarium. Exposure of seeds samples to different radiation doses showed that a dose level of 6.0 kGy could be considered as a sufficient dose for decontamination of the tested samples. Seven radioresistant isolates were identified as Rhizopus oryzae, Rhizopus stolonifer, Penicillium chrysogenum and Penicillium corylophillum. All the herb samples were found to be free from aflatoxins B 1, B 2, G 1, G 2 and ochratoxin A. One mold isolate was identified as Aspergillus flavus could produce aflatoxin B 1 and G 1. None of the isolated radioresistant strains could produce mycotoxins. The water activities of seeds were slightly decreased by the storage time and the seeds needed to be stored at relative humidity not more than 85%. The addition of extract volatile and fixed oil from tested seeds to the medium stimulated the growth of isolated Aspergillus sp.

  17. Effect of six Chinese spices on heterocyclic amine profiles in roast beef patties by ultra performance liquid chromatography-tandem mass spectrometry and principal component analysis.

    PubMed

    Zeng, Maomao; He, Zhiyong; Zheng, Zongping; Qin, Fang; Tao, Guanjun; Zhang, Shuang; Gao, Yahui; Chen, Jie

    2014-10-08

    The effects of Chinese spices on the profiles of 17 heterocyclic amines (HAs) from seven HA categories were investigated in roast beef patties using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) and principal component analysis. Three groups of HAs, imidazopyridines (PhIP, DMIP, and 1,5,6-TMIP), imidazoquinoxalines (MeIQx and 4,8-DiMeIQx), and β-carbolines (harman and norharman), were detected and quantified in all of the samples. The results demonstrated that the total HA and imidazopyridine profiles could clearly be affected by 1% pricklyash peel (14.1 ± 0.76 and 6.06 ± 0.32 ng/g), chilli (41.0 ± 0.01 and 23.0 ± 0.52 ng/g), and cumin (59.9 ± 2.44 and 31.1 ± 3.06 ng/g), in comparison with control values of 21.8 ± 2.40 and 14.3 ± 2.04 ng/g, respectively. The difference was only significant (p < 0.05) for cumin. The imidazoquinoxaline profile was significantly (p < 0.05) affected by 1% pricklyash peel (0.57 ± 0.05 ng/g) and cumin (2.36 ± 0.20 ng/g) compared to the control (1.16 ± 0.11 ng/g). The β-carboline profile was only markedly (p < 0.05) affected by 1% cumin (26.4 ± 0.82 ng/g) compared to the control (6.26 ± 0.26 ng/g). In general, pricklyash peel inhibited HA formation, whereas star anise, fennel, cumin, chilli, and black pepper promoted HA formation. The findings could facilitate the selection of spices in meat processing to minimize HA formation.

  18. The Phytotoxicity of Designated Pollutants

    DTIC Science & Technology

    1982-11-01

    lengths. Results paralleled emer- gence; a reduction of growth occurred when fuel was applied (Table 46). Shoots appeared equally stressed since root...1954, Mode of action of phytotoxic oils, Weeds 3:55-65. Williams, G. R., E. Cumins , A. C. Gardner, M. Palmier, and T. Rubidge, 1981, The growth of

  19. In vitro antifungal effect of black cumin seed quinones against dairy spoilage yeasts at different acidity levels.

    PubMed

    Halamova, Katerina; Kokoska, Ladislav; Flesar, Jaroslav; Sklenickova, Olga; Svobodova, Blanka; Marsik, Petr

    2010-12-01

    The antiyeast activity of the black cumin seed (Nigella sativa) quinones dithymoquinone, thymohydroquinone (THQ), and thymoquinone (TQ) were evaluated in vitro with a broth microdilution method against six dairy spoilage yeast species. Antifungal effects of the quinones were compared with those of preservatives commonly used in milk products (calcium propionate, natamycin, and potassium sorbate) at two pH levels (4.0 and 5.5). THQ and TQ possessed significant antiyeast activity and affected the growth of all strains tested at both pH levels, with MICs ranging from 8 to 128 μg/ml. With the exception of the antibiotic natamycin, the inhibitory effects of all food preservatives against the yeast strains tested in this study were strongly affected by differences in pH, with MICs of ≥16 and ≥512 μg/ml at pH 4.0 and 5.5, respectively. These findings suggest that HQ and TQ are effective antiyeast agents that could be used in the dairy industry as chemical preservatives of natural origin.

  20. Amino acid composition and biological effects of supplementing broad bean and corn proteins with Nigella sativa (black cumin) cake protein.

    PubMed

    al-Gaby, A M

    1998-10-01

    The biological effects of supplementing broad bean (Vicia faba) or corn (Zea maize) meal protein with black cumin (Nigella sativa) cake protein as well as their amino acid composition were investigated. The percentage of total protein content of Nigella cake was 22.7%. Lysine is existent in abundant amounts in faba meal protein, while leucine is the most abundant in corn meal protein (chemical score = 156) and valine is higher in Nagella cake protein. compared with rats fed sole corn or faba meal protein, substitution of 25% of corn or faba meal protein with Nigella cake protein in the diet remarkably raised the growth rate of rats and resulted in significant higher levels of rat total serum lipids and triglycerides. Also, the supplemented diet caused significant increases in serum total protein and its two fractions albumin and globulin and insignificantly increase the activity of serum phosphatases and transaminases within normal ranges. The supplementation did not have any adverse nutritional effects in the levels of lipid fractions in the serum.

  1. Analysis and antibacterial activity of Nigella sativa essential oil formulated in microemulsion system.

    PubMed

    Shaaban, Hamdy A; Sadek, Zainab; Edris, Amr E; Saad-Hussein, Amal

    2015-01-01

    The Essential oil (EO) of Nigella sativa (black cumin) was extracted from the crude oil and the volatile constituents were characterized using gas chromatographic analysis. The EO was formulated in water-based microemulsion system and its antibacterial activity against six pathogenic bacteria was evaluated using the agar well diffusion method. This activity was compared with two other well known biologically active natural and synthetic antimicrobials namely eugenol and Ceftriaxone(®). Results showed that N. sativa EO microemulsion was highly effective against S. aureus, B. cereus and S. typhimurium even at the lowest tested concentration of that EO in the microemulsion (100.0 μg/well). Interestingly, the EO microemulsion showed higher antibacterial activity than Ceftriaxone solution against S. typhimurium at 400.0 μg/well and almost comparable activity against E. coli at 500.0 μg/well. No activity was detected for the EO microemulsion against L. monocytogenes and P. aeruginosa. Eugenol which was also formulated in microemulsion was less effective than N. sativa EO microemulsion except against P. aeruginosa. The synthetic antibiotic (Ceftriaxone) was effective against most of the six tested bacterial strains. This work is the first report revealing the formulation of N. sativa EO in microemulsion system and investigating its antibacterial activity. The results may offer potential application of that water-based microemulsion in controlling the prevalence of some pathogenic bacteria.

  2. Mutagenic activity of south Indian food items.

    PubMed

    Sivaswamy, S N; Balachandran, B; Balanehru, S; Sivaramakrishnan, V M

    1991-08-01

    Dietary components and food dishes commonly consumed in South India were screened for their mutagenic activity. Kesari powder, calamus oil, palm drink, toddy and Kewra essence were found to be strongly mutagenic; garlic, palm oil, arrack, onion and pyrolysed portions of bread toast, chicory powder were weakly mutagenic, while tamarind and turmeric were not. Certain salted, sundried and oil fried food items were also mutagenic. Cissus quadrangularis was mutagenic, while 'decoctions' of cumin seeds, aniseeds and ginger were not. Several perfumes, essential oils and colouring agents, which are commonly used were also screened and many of them exhibited their mutagenic potential by inducing the 'reverse mutation' in Salmonella typhimurium tester strains.

  3. Biochemical characterization, anti-inflammatory properties and ulcerogenic traits of some cold-pressed oils in experimental animals.

    PubMed

    Ibrahim, Faten M; Attia, Hanan Naeim; Maklad, Yousreya Aly Aly; Ahmed, Kawkab A; Ramadan, Mohamed F

    2017-12-01

    Cold-pressed oils (CPO) are commercially available in the market and characterized by their health-promoting properties. Clove oil (CLO), coriander seed oil (COO) and black cumin oil (BCO) were evaluated for their bioactive lipids. Pharmacological screening was performed to evaluate acute toxicity, anti-inflammatory and ulcerogenic effects as well as histopathological changes in tissues of albino rats fed with CPO. Fatty acids, tocols and total phenolics were analyzed. The acute toxicity test for each CPO was estimated during 14 d. Carrageenan-induced rat paw oedema was used for assessment of anti-inflammatory activity of CPO. Animals were fasted overnight, and via oral gavage given indomethacin (10 mg/kg) or CPO (400 mg/kg) to investigate ulcerogenecity. Histopathological changes in liver, kidney, heart, spleen and stomach were screened. Amounts of α-, β-, γ- and δ-tocopherols in CLO were 1495, 58, 4177 and 177 mg/kg oil, respectively. In COO, α, β, γ and δ-tocopherols were 10.0, 18.2, 5.1 and 34.8%, respectively. In BCO, β-tocotrienol was the main constituent. CLO, COO and BCO contained 4.6, 4.2 and 3.6 mg GAE/g, respectively. Acute toxicity test determined that 400 mg/kg of CPO to be used. In the carrageenan model of inflammation, pretreatment of rats with indomethacin (10 mg/kg) or CLO (400 mg/kg) induced a significant (p < 0.05) reduction by 31.3 and 27.4%, respectively, in rat paw oedema as compared with the carrageenan-treated group. Indomethacin induced a significant ulcerogenic effect with an ulcer index of 19. Oral treatment of CPO showed no ulcerogenic effect, wherein no histopathological changes were observed. CPO, particularly CLO, could minimize acute inflammation.

  4. Nigella sativa Relieves the Altered Insulin Receptor Signaling in Streptozotocin-Induced Diabetic Rats Fed with a High-Fat Diet.

    PubMed

    Balbaa, Mahmoud; El-Zeftawy, Marwa; Ghareeb, Doaa; Taha, Nabil; Mandour, Abdel Wahab

    2016-01-01

    The black cumin (Nigella sativa) "NS" or the black seeds have many pharmacological activities such as antioxidant, anticarcinogenic, antihypertensive, and antidiabetic properties. In this work, streptozotocin-induced diabetic rats fed with a high-fat diet were treated daily with NS oil (NSO) in order to study the effect on the blood glucose, lipid profile, oxidative stress parameters, and the gene expression of some insulin receptor-induced signaling molecules. This treatment was combined also with some drugs (metformin and glimepiride) and the insulin receptor inhibitor I-OMe-AG538. The administration of NSO significantly induced the gene expression of insulin receptor compared to rats that did not receive NSO. Also, it upregulated the expression of insulin-like growth factor-1 and phosphoinositide-3 kinase, whereas the expression of ADAM-17 was downregulated. The expression of ADAM-17 is corroborated by the analysis of TIMP-3 content. In addition, the NSO significantly reduced blood glucose level, components of the lipid profile, oxidative stress parameters, serum insulin/insulin receptor ratio, and the tumor necrosis factor-α, confirming that NSO has an antidiabetic activity. Thus, the daily NSO treatment in our rat model indicates that NSO has a potential in the management of diabetes as well as improvement of insulin-induced signaling.

  5. A simultaneous screening and quantitative method for the multiresidue analysis of pesticides in spices using ultra-high performance liquid chromatography-high resolution (Orbitrap) mass spectrometry.

    PubMed

    Goon, Arnab; Khan, Zareen; Oulkar, Dasharath; Shinde, Raviraj; Gaikwad, Suresh; Banerjee, Kaushik

    2018-01-12

    A novel screening and quantitation method is reported for non-target multiresidue analysis of pesticides using ultra-HPLC-quadrupole-Orbitrap mass spectrometry in spice matrices, including black pepper, cardamom, chili, coriander, cumin, and turmeric. The method involved sequential full-scan (resolution = 70,000), and variable data independent acquisition (vDIA) with nine consecutive fragmentation events (resolution = 17,500). Samples were extracted by the QuEChERS method. The introduction of an SPE-based clean-up step through hydrophilic-lipophilic-balance (HLB) cartridges proved advantageous in minimizing the false negatives. For coriander, cumin, chili, and cardamom, the screening detection limit was largely at 2 ng/g, while it was 5 ng/g for black pepper, and turmeric. When the method was quantitatively validated for 199 pesticides, the limit of quantification (LOQ) was mostly at 10 ng/g (excluding black pepper, and turmeric with LOQ = 20 ng/g) with recoveries within 70-120%, and precision-RSDs <20%. Furthermore, the method allowed the identification of suspected non-target analytes through retrospective search of the accurate mass of the compound-specific precursor and product ions. Compared to LC-MS/MS, the quantitative performance of this Orbitrap-MS method had agreements in residue values between 78-100%. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Cancer chemopreventive potential of volatile oil from black cumin seeds, Nigella sativa L., in a rat multi-organ carcinogenesis bioassay.

    PubMed

    Salim, Elsayed I

    2010-09-01

    Nigella sativa (N. sativa) is a herbal plant of the Ranunculaceae family that has been widely used for various medicinal and nutritional purposes. Volatile oil extracts along with its major constituents, such as thymoquinone, have recently attracted considerable attention for their antioxidant, immunoprotective and antitumor properties. The present study was conducted to assess the chemopreventive potential of crude oils in N. sativa on tumor formation using a well-established rat multi-organ carcinogenesis model featuring initial treatment with five different carcinogens. Post-initiation administration of 1000 or 4000 ppm N. sativa volatile oil in the diet of male Wistar rats for 30 weeks significantly reduced malignant and benign colon tumor sizes, incidences and multiplicities. The treatment also significantly decreased the incidences and multiplicities of tumors in the lungs and in different parts of the alimentary canal, particularly the esophagus and forestomach. Bromodeoxyuridine labeling indices, reflecting cell proliferation were significantly decreased in various organs and lesions after treatment with the two doses of N. sativa. The plasma levels of insulin growth factor, triglycerides and prostaglandin E2 were also altered. The findings show, for the first time, that N. sativa administration exerts potent inhibitory effects on rat tumor development and on cellular proliferation in multiple organ sites. In particular, the ability to significantly inhibit murine colon, lung, esophageal and forestomach tumors was demonstrated in the post-initiation phase, with no evidence of clinical side effects. The mechanisms are likely to be related to suppression of cell proliferation.

  7. Cancer chemopreventive potential of volatile oil from black cumin seeds, Nigella sativa L., in a rat multi-organ carcinogenesis bioassay

    PubMed Central

    SALIM, ELSAYED I.

    2010-01-01

    Nigella sativa (N. sativa) is a herbal plant of the Ranunculaceae family that has been widely used for various medicinal and nutritional purposes. Volatile oil extracts along with its major constituents, such as thymoquinone, have recently attracted considerable attention for their antioxidant, immunoprotective and antitumor properties. The present study was conducted to assess the chemopreventive potential of crude oils in N. sativa on tumor formation using a well-established rat multi-organ carcinogenesis model featuring initial treatment with five different carcinogens. Post-initiation administration of 1000 or 4000 ppm N. sativa volatile oil in the diet of male Wistar rats for 30 weeks significantly reduced malignant and benign colon tumor sizes, incidences and multiplicities. The treatment also significantly decreased the incidences and multiplicities of tumors in the lungs and in different parts of the alimentary canal, particularly the esophagus and forestomach. Bromodeoxyuridine labeling indices, reflecting cell proliferation were significantly decreased in various organs and lesions after treatment with the two doses of N. sativa. The plasma levels of insulin growth factor, triglycerides and prostaglandin E2 were also altered. The findings show, for the first time, that N. sativa administration exerts potent inhibitory effects on rat tumor development and on cellular proliferation in multiple organ sites. In particular, the ability to significantly inhibit murine colon, lung, esophageal and forestomach tumors was demonstrated in the post-initiation phase, with no evidence of clinical side effects. The mechanisms are likely to be related to suppression of cell proliferation. PMID:22966405

  8. Effect of thyme/cumin essential oils and butylated hydroxyl anisole/butylated hydroxyl toluene on physicochemical properties and oxidative/microbial stability of chicken patties.

    PubMed

    Sariçoban, Cemalettin; Yilmaz, Mustafa Tahsin

    2014-02-01

    In this study, effects of thyme/cumin essential oils (EO) and butylated hydroxyl anisole (BHA)/butylated hydroxyl toluene (BHT) on physicochemical properties and storage stability of chicken patties were compared in different storage periods (0, 3, 7, 14, 21, and 28 d). It was found that there were significant (P < 0.05) differences between physicochemical properties of patty samples treated with EO and the synthetic antioxidants. The EO showed similar performance to those of BHA and BHT in limiting TBARS values of chicken patty samples. Similarity in performance was also the case for microbial stability (total aerobic mesophilic, psychrotrophic, lactic acid, and coliform bacteria as well as molds and yeasts); namely, their effects were significant (P < 0.05). Effect of thyme EO was significant (P < 0.05) and remarkable, not allowing any coliform bacteria to grow in the samples. Given that EO were obtained from natural sources, the data suggested that the EO might be more useful than their synthetic counterparts, BHA and BHT, as additives for chicken patties to maintain oxidative/microbial stability and increase shelf life.

  9. The cardioprotective effect of thymoquinone on ischemia-reperfusion injury in isolated rat heart via regulation of apoptosis and autophagy.

    PubMed

    Xiao, Junhui; Ke, Zun-Ping; Shi, Yan; Zeng, Qiutang; Cao, Zhe

    2018-06-22

    Thymoquinone (TQ), as the active constituents of black cumin (Nigella sativa) seed oil, has been reported to have potential protective effects on the cardiovascular system. This study aimed to investigate the effects and the underlying mechanisms of TQ on myocardial ischemia-reperfusion (I/R) injury in Langendorff-perfused rat hearts. Wister rat hearts were subjected to I/R and the experimental group were pretreated with TQ prior to I/R. Hemodynamic parameters, myocardial infarct size, cardiac marker enzymes, superoxide dismutase (SOD), malondialdehyde (MDA) content, and cardiomyocyte apoptosis were assayed. Compared with the untreated group, TQ preconditioning significantly improved cardiac function, reduced infarct size, decreased cardiac lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) levels, suppressed enedoxidative stress, and apoptosis. In addition, TQ treatment promoted autophagy, which was partially reversed by chloroquine (CQ), a kind of autophagy blocker. Our study suggests that TQ can protect heart against I/R injury, which is associated with anti-oxidative and anti-apoptotic effects through activation of autophagy. © 2018 Wiley Periodicals, Inc.

  10. Protein Bread Fortification with Cumin and Caraway Seeds and By-Product Flour.

    PubMed

    Sayed Ahmad, Bouchra; Talou, Thierry; Straumite, Evita; Sabovics, Martins; Kruma, Zanda; Saad, Zeinab; Hijazi, Akram; Merah, Othmane

    2018-02-25

    Malnutrition continues to be a key health problem in developing regions. The valorization of food waste appears as an ideal way to prevent malnutrition and improve people's access to food. Cumin ( Cuminum cyminum L.) and caraway ( Carum carvi L.) oilseeds are commonly used for cuisine and medicinal purposes. However, remaining cakes after oil extraction are usually underutilized. In order to assess the usefulness of these by-products in food applications, this study investigated the effect of their addition to protein bread formulations. Different levels (2, 4 and 6%) of whole seeds and cakes flour were used in the study. Fortified protein bread samples were compared to control protein bread and evaluated for their sensory, color, moisture, hardness properties, nutritional values as well as their biological activity. Results indicated that bread fortification shows a significant effect on bread properties depending on fortification level. A higher acceptability was observed specially for bread fortified with by-products flour. Increased tendencies of color darkness, moisture content, bread hardness, nutritional values as well as total phenolic content and radical scavenging activity compared to control bread were observed as the percentage of fortification increased in both cases. The overall results showed that the addition of cumin and caraway seeds and by-product flour can improve the antioxidant potential and overall quality of protein bread.

  11. Nigella sativa: reduces the risk of various maladies.

    PubMed

    Butt, Masood Sadiq; Sultan, Muhammad Tauseef

    2010-08-01

    Coinage of terms like nutraceuticals, functional, and pharma foods has diverted the attention of human beings to where they are seeking more natural cures. Though pharmaceutical drugs have been beneficial for human health and have cured various diseases but they also impart some side effects. Numerous plants have been tested for their therapeutic potential; Nigella sativa, commonly known as black cumin, is one of them. It possesses a nutritional dense profile as its fixed oil (lipid fraction), is rich in unsaturated fatty acids while essential oil contains thymoquinone and carvacrol as antioxidants. N. sativa seeds also contain proteins, alkaloids (nigellicines and nigelledine), and saponins (alpha-hederin) in substantial amounts. Recent pharmacological investigations suggested its potential role, especially for the amelioration of oxidative stress through free radical scavenging activity, the induction of apoptosis to cure various cancer lines, the reduction of blood glucose, and the prevention of complications from diabetes. It regulates hematological and serological aspects and can be effective in dyslipidemia and respiratory disorders. Moreover, its immunopotentiating and immunomodulating role brings balance in the immune system. Evidence is available supporting the utilization of Nigella sativa and its bioactive components in a daily diet for health improvement. This review is intended to focus on the composition of Nigella sativa and to elaborate its possible therapeutic roles as a functional food to prevent an array of maladies.

  12. Antifungal and Antiochratoxigenic Activities of Essential Oils and Total Phenolic Extracts: A Comparative Study

    PubMed Central

    EL Khoury, Rachelle; Atoui, Ali; Mathieu, Florence; Kawtharani, Hiba; EL Khoury, Anthony; Maroun, Richard G.; EL Khoury, Andre

    2017-01-01

    This study is intended to prevent ochratoxin A (OTA) production by Aspergillus carbonarius S402 using essential oils (EOs) and total phenolic compounds extracted from plants and herbs. The EOs used in this study are the following: bay leaves, cumin, fenugreek, melissa, mint, and sage. As for the phenolic compounds, they were extracted from bay leaves, cumin, fenugreek, melissa, mint, sage, anise, chamomile, fennel, rosemary, and thyme. The experiments were conducted on Synthetic Grape Medium (SGM) medium at 28 °C for 4 days. OTA was extracted from the medium with methanol and quantified using HPLC (High Performance Liquid Chromatography). Results showed that EOs had a greater impact than the total phenolic extracts on the OTA production. Reduction levels ranged between 25% (sage) and 80% (melissa) for the EOs at 5 µL mL−1, and 13% (thyme) and 69% (mint) for the phenolic extracts. Although they did not affect the growth of A. carbonarius, total phenolic extracts and EOs were capable of partially reducing OTA production. Reduction levels depended on the nature of the plants and the concentration of the EOs. Reducing OTA with natural extracts could be a solution to prevent OTA production without altering the fungal growth, thus preserving the natural microbial balance. PMID:28698493

  13. Protein Bread Fortification with Cumin and Caraway Seeds and By-Product Flour

    PubMed Central

    Sayed Ahmad, Bouchra; Talou, Thierry; Straumite, Evita; Sabovics, Martins; Kruma, Zanda; Saad, Zeinab; Hijazi, Akram

    2018-01-01

    Malnutrition continues to be a key health problem in developing regions. The valorization of food waste appears as an ideal way to prevent malnutrition and improve people’s access to food. Cumin (Cuminum cyminum L.) and caraway (Carum carvi L.) oilseeds are commonly used for cuisine and medicinal purposes. However, remaining cakes after oil extraction are usually underutilized. In order to assess the usefulness of these by-products in food applications, this study investigated the effect of their addition to protein bread formulations. Different levels (2, 4 and 6%) of whole seeds and cakes flour were used in the study. Fortified protein bread samples were compared to control protein bread and evaluated for their sensory, color, moisture, hardness properties, nutritional values as well as their biological activity. Results indicated that bread fortification shows a significant effect on bread properties depending on fortification level. A higher acceptability was observed specially for bread fortified with by-products flour. Increased tendencies of color darkness, moisture content, bread hardness, nutritional values as well as total phenolic content and radical scavenging activity compared to control bread were observed as the percentage of fortification increased in both cases. The overall results showed that the addition of cumin and caraway seeds and by-product flour can improve the antioxidant potential and overall quality of protein bread. PMID:29495324

  14. Nigella sativa (black cumin) ameliorates potassium bromate-induced early events of carcinogenesis: diminution of oxidative stress.

    PubMed

    Khan, Naghma; Sharma, Sonia; Sultana, Sarwat

    2003-04-01

    Potassium bromate (KBrO3) is a potent nephrotoxic agent. In this paper, we report the chemopreventive effect of Nigella sativa (black cumin) on KBrO3-mediated renal oxidative stress, toxicity and tumor promotion response in rats. KBrO3 (125 mg/kg body weight, intraperitoneally) enhances lipid peroxidation, gamma-glutamyl transpeptidase, hydrogen peroxide and xanthine oxidase with reduction in the activities of renal antioxidant enzymes and renal glutathione content. A marked increase in blood urea nitrogen and serum creatinine has also been observed. KBrO3 treatment also enhances ornithine decarboxylase (ODC) activity and [3H] thymidine incorporation into renal DNA. Prophylaxis of rats orally with Nigella sativa extract (50 mg/kg body weight and 100 mg/kg body weight) resulted in a significant decrease in renal microsomal lipid peroxidation (P < 0.001), gamma-glutamyl transpeptidase (P < 0.001), H2O2 (P < 0.001) and xanthine oxidase (P < 0.05). There was significant recovery of renal glutathione content (P < 0.01) and antioxidant enzymes (P < 0.001). There was also reversal in the enhancement of blood urea nitrogen, serum creatinine, renal ODC activity and DNA synthesis (P < 0.001). Data suggest that Nigella sativa is a potent chemopreventive agent and may suppress KBrO3-mediated renal oxidative stress, toxicity and tumour promotion response in rats.

  15. Polysaccharide of Black cumin (Nigella sativa) modulates molecular signaling cascade of gastric ulcer pathogenesis.

    PubMed

    Manjegowda, Srikanta Belagihalli; Rajagopal, Harsha Mysore; Dharmesh, Shylaja Mallaiah

    2017-08-01

    Gastric ulcer is a multi-step disease and healing requires a complex process including repair and re-architecture of gastric mucosa with the involvement of molecular events. Current study was designed to understand the gastric ulcer healing mechanism of rhamnogalacturonan-I type pectic polysaccharide of black cumin (BCPP) utilizing acetic acid induced gastric ulcers in rats. BCPP fed groups at 200mg/kg b.w. for 10days showed up to 85% healing of gastric ulcers with modulation of key molecular events involved in ulcer healing process such as increase in gastric mucin content, cyclooxygenase-2 (Cox-2) and prostaglandin E 2 (PGE 2 ). The increase in extracellular signal-regulated kinase-2 (ERK-2) indicated that, BCPP could induce PGE-2 synthesis by increasing ERK-2 mediated COX-2 activity. Increase in matrix metalloproteinase-2 (MMP-2) and decrease in MMP-9 levels in BCPP treated groups indicated differential regulation of MMP-2 and 9, an essential event required for gastric mucosal re-modulation. BCPP containing bound phenolics (26mg/g) might have also played a role in increasing speed and quality of ulcer healing by inhibiting H + , K + -ATPase and decreasing free radical mediated oxidation and cellular damages. Overall, studies showed that the polysaccharide can mediate ulcer healing by modulating signaling pathways involved in either ulcer aggravation or healing process. Copyright © 2017. Published by Elsevier B.V.

  16. Protective effect of thymoquinone, the main component of Nigella Sativa, against diazinon cardio-toxicity in rats.

    PubMed

    Danaei, Gholam Hassan; Memar, Bahram; Ataee, Ramin; Karami, Mohammad

    2018-04-12

    Several studies have shown that oxidative stress and cell damage can occur at very early stages of diazinon (DZN) exposure. The present study was designed to determine the beneficial effect of thymoquinone (Thy), the main component of Nigella sativa (black seed or black cumin), against DZN cardio-toxicity in rats. In the present experimental study, 48 male Wistar rats were randomly divided into six groups: control (corn oil gavages), DZN gavages (20 mg/kg/day), Thy gavages (10 mg/kg/day) and Thy + DVN gavages (2.5, 5 and 10 mg/kg/day). Treatments were continued for 28 days, then the animals were anesthetized by ether and superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), lactate dehydrogenize (LDH) and glutathione peroxide (GPX) activity was evaluated. In addition, glutathione (GSH) and malondialdehyde (MDA) the heart tissue and creatinephosphokinase-MB (CPK-MB) and troponin (TPI) levels and cholinesterase activity in the blood were evaluated. DZN-induced oxidative damage and elevated the levels of the cardiac markers CK-MB, TPI, MDA and LDH and decreased SOD, CAT and cholinesterase activity and GSH level compared with the control group. Treatment with Thy reduced DZN cardio-toxicity and cholinesterase activity. The success of Thy supplementation against DZN toxicity can be attributed to the antioxidant effects of its constituents. Administration of Thy as a natural antioxidant decreased DZN cardio-toxicity and improved cholinesterase activity in rats through the mechanism of free radical scavenging.

  17. Chemopreventive effects of Cuminum cyminum in chemically induced forestomach and uterine cervix tumors in murine model systems.

    PubMed

    Gagandeep; Dhanalakshmi, Sivanandhan; Méndiz, Ester; Rao, Agra Ramesha; Kale, Raosaheb Kathalupant

    2003-01-01

    Lately, a strong correlation has been established between diet and cancer. For ages, cumin has been a part of the diet. It is a popular spice regularly used as a flavoring agent in a number of ethnic cousins. In the present study, cancer chemopreventive potentials of different doses of a cumin seed-mixed diet were evaluated against benzo(a)pyrene [B(a)P]-induced forestomach tumorigenesis and 3-methylcholanthrene (MCA)-induced uterine cervix tumorigenesis. Results showed a significant inhibition of stomach tumor burden (tumors per mouse) by cumin. Tumor burden was 7.33 +/- 2.10 in the B(a)P-treated control group, whereas it reduced to 3.10 +/- 0.57 (P < 0.001) by a 2.5% dose and 3.11 +/- 0.60 (P <0.001) by a 5% dose of cumin seeds. Cervical carcinoma incidence, compared with the MCA-treated control group (66.67%), reduced to 27.27% (P < 0.05) by a diet of 5% cumin seeds and to 12.50% (P < 0.05) by a diet of 7.5% cumin seeds. The effect of 2.5 and 5% cumin seed-mixed diets was also examined on carcinogen/xenobiotic metabolizing phase I and phase II enzymes, antioxidant enzymes, glutathione content, lactate dehydrogenase (LDH), and lipid peroxidation in the liver of Swiss albino mice. Levels of cytochrome P-450 (cyt P-450) and cytochrome b5 (cyt b(5)) were significantly augmented (P < 0.05) by the 2.5% dose of cumin seed diet. The levels of cyt P-450 reductase and cyt b(5) reductase were increased (significance level being from P < 0.05 to P < 0.01) by both doses of cumin. Among the phase II enzymes, glutathione S-transferase specific activity increased (P < 0.005) by the 5% dose, whereas that of DT-diaphorase increased significantly (P < 0.05) by both doses used (2.5 and 5%). In the antioxidant system, significant elevation of the specific activities of superoxide dismutase (P < 0.01) and catalase (P < 0.05) was observed with the 5% dose of cumin. The activities of glutathione peroxidase and glutathione reductase remained unaltered by both doses of cumin. The level of reduced glutathione measured as nonprotein sulfhydryl content was elevated (significance level being from P < 0.05 to P < 0.01) by both doses of cumin. Lipid peroxidation measured as formation of MDA production showed significant inhibition (P < 0.05 to P < 0.01) by both doses of cumin. LDH activity remained unaltered by both doses of cumin. The results strongly suggest the cancer chemopreventive potentials of cumin seed and could be attributed to its ability to modulate carcinogen metabolism.

  18. Nutraceuticals of anti-inflammatory activity as complementary therapy for rheumatoid arthritis.

    PubMed

    Al-Okbi, Sahar Y

    2014-09-01

    Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by elevated oxidative stress and inflammatory biomarkers. The severe side effects of drug used during such disease necessitate the search for new and safe approaches. Food is a rich source of antioxidants and anti-inflammatory bioactive constituents including phenolic compounds, polyunsaturated fatty acids, phytosterols, toccopherols, and carotenoids. We have a series of publications dealing with the anti-inflammatory activity of different food extracts (as nutraceuticals) in experimental animals (acute and chronic inflammation model) and in clinical study (RA patients). Fish oil, primrose oil, extracts of black cumin, fenugreek, liquorice, coriander, tomato, carrot, sweet potato, broccoli, green tea, rosemary, hazelnut, walnut, wheat germ, and date in addition to the probiotic Bifidobacterium bifidum were the nutraceuticals studied. During these studies, changes in inflammatory biomarkers (erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), seromucoids, fibrinogen, tumor necrosis factor-α (TNF-α), prostaglandin E2), oxidative stress (malondialdehyde), antioxidant status (total antioxidant capacity, vitamin C, vitamin E, retinol, β-carotene), the level of copper (Cu) and zinc (Zn) and colonic microflora in response to the administration of nutraceuticals have been assessed. Results of these studies showed that the majority of nutraceuticals studied possess beneficial effect toward chronic inflammatory diseases, which might be due to the presence of one or more of the above-mentioned phytochemicals. Anti-inflammatory and antioxidant nutraceuticals may serve as complementary medicine for the management of RA. © The Author(s) 2012.

  19. Potential use of cuminic acid as a botanical fungicide against Valsa mali.

    PubMed

    Wang, Yong; Sun, Yang; Han, LiRong; Zhang, Xing; Feng, Juntao

    2017-05-01

    Valsa canker caused by Valsa mali is commonly present in eastern Asia and cause large economic losses. Because of limited agricultural measures and chemical residues of commonly used fungicides there is an urgent need of alternative plant protecting agents. On this background the activity of cuminic acid, a plant extract from the seed of Cuminum cyminum L, was assessed. The median effective concentration (EC 50 ) values for inhibition of mycelial growth of seven V. mali strains ranged from 3.046 to 8.342 μg/mL, with an average EC 50 value of 4.956 ± 0.281 μg/mL. The antifungal activity was the direct activity of cuminic acid instead of the influence on the pH of media by cuminic acid. After treated with cuminic acid, mycelia dissolved with decreased branches and swelling; cell membrane permeability increased while pectinases activity decreased significantly. Moreover, peroxidase (POD) activity of the apple leaves increased after treated with cuminic acid. Importantly, on detached branches of apple tree, cuminic acid exhibited both protective and curative activity. These results indicated that cuminic acid not only showed the antifungal activity, but also could improve the defense capacity of the plants. Taken together, cuminic acid showed the potential as a natural alternative to commercial fungicides or a lead compound to develop new fungicides for the control of Valsa canker. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Odorant-binding protein-based identification of natural spatial repellents for the African malaria mosquito Anopheles gambiae.

    PubMed

    Kröber, Thomas; Koussis, Konstantinos; Bourquin, Martine; Tsitoura, Panagiota; Konstantopoulou, Maria; Awolola, Taiwo Sam; Dani, Francesca R; Qiao, Huili; Pelosi, Paolo; Iatrou, Kostas; Guerin, Patrick M

    2018-05-01

    There is increasing interest in the development of effective mosquito repellents of natural origin to reduce transmission of diseases such as malaria and yellow fever. To achieve this we have employed an in vitro competition assay involving odorant-binding proteins (OBPs) of the malaria mosquito, Anopheles gambiae, with a predominantly female expression bias to identify plant essential oils (EOs) containing bioactive compounds that target mosquito olfactory function. EOs and their fractions capable of binding to such OBPs displayed repellence against female mosquitoes in a laboratory repellent assay. Repellent EOs were subjected to gas chromatographic analysis linked to antennogram (EAG) recordings from female A. gambiae to identify the biologically active constituents. Among these compounds cumin alcohol, carvacrol, ethyl cinnamate and butyl cinnamate proved as effective as DEET at an equivalent dose in the repellent assay, and combinations of carvacrol with either butyl cinnamate or cumin alcohol proved to be significantly more effective than DEET in the assay. When tested as spatial repellents in experimental shelters housing sleeping humans in northern Nigeria a binary mixture of carvacrol plus cumin alcohol caused mosquitoes to leave shelters in significantly higher numbers to those induced by DEET in female Anopheles spp. and in numbers equivalent to that of DEET in Culex spp. mosquitoes. These findings indicate an approach for the identification of biologically active molecules of natural origin serving as repellents for mosquitoes. Copyright © 2018 Elsevier Ltd. All rights reserved.

  1. Effects of cumin extract on oxLDL, paraoxanase 1 activity, FBS, total cholesterol, triglycerides, HDL-C, LDL-C, Apo A1, and Apo B in in the patients with hypercholesterolemia

    PubMed Central

    Samani, Keihan Ghatreh; Farrokhi, Effat

    2014-01-01

    Objectives Paraoxanase 1 (PON1) plays a protective role against the oxidative modification of plasma lipoproteins and hydrolyzes lipid peroxides in human atherosclerotic lesions. Cumin is the dried seed of the herb Cuminumcyminum that is known as Zeera in Iran. Cumin seeds contain flavonoids which are now generally recognized to have antioxidant activity and improve the antioxidant system. So, they possibly modify PON1 activity and oxidized low density lipoprotein (oxLDL) level. The present study was aimed to evaluate the effects of cumin extract supplementation on oxLDL, paraoxanase 1 activity, FBS, total cholesterol, triglycerides, High density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (Apo A1), and apolipoprotein B (Apo B)in the patients with hypercholesterolemia. Methodology A fasting venous blood sample was obtained from the voluntary persons before and 45±3 days after taking cumin. Glucose, total cholesterol, and triglycerides were assayed using standard enzymatic procedures. HDL-Cand LDL-C were measured by direct method and ApoA1 and ApoB levels by immunoturbidimeteric methods. The levels of arylesterase and paraoxanase activities in the samples were measured by photometry methods and oxLDL by enzyme-linked immunosorbent assay (ELISA) method. 3 to 5 drops of cumin extract were added to the patient’s diet three times a day based on manufacturer’s instruction for 45±3 days. The biochemical parameters were compared before and after taking cumin. Data were analyzed using paired Student’s t-test in SPSS statistical software (version 11.5). Results The results demonstrated that there was a significant decrease in the level of oxLDL after receiving cumin. Paraoxonase and arylesterase activities increased in serum after taking cumin extract. Conclusion Based on the results, cumin reduces oxLDL level and increases both paraoxonase and arylesterase activity. PMID:24899878

  2. Synergistic antibacterial activity of the essential oil of Cuminum cyminum L. seed and nisin in a food model.

    PubMed

    Pajohi, M R; Tajik, H; Farshid, A A; Hadian, M

    2011-04-01

    To investigate effects of various concentrations of the essential oil of Cuminum cyminum L. seed alone and in combination with nisin on survival of vegetative forms of Bacillus cereus and Bacillus subtilis in a food model (commercial barley soup) and their ultrastructure. Gas chromatography-mass spectrometry analysis indicated that cumin aldehyde (29·02%) and α-terpinen-7-al (20·70%) constituted the highest amount of the essential oil. The lowest concentration of the essential oil significantly affected the growth of the bacteria at 8°C but not at 25°C. Synergistic effect of the essential oil in combination with the lowest concentration of nisin was observed on the bacteria at 8°C. Evaluation of the sensory properties showed that concentration of 0·15 μl ml−1 of the essential oil was the most acceptable.  The essential oil of C. cyminum L. seed showed the most bactericidal effects on B. cereus at 8°C. Ultrastructural studies of vegetative cells confirmed the synergistic destructive effects of the essential oil and nisin on membrane and cell wall of the bacteria.

  3. Various extraction and analytical techniques for isolation and identification of secondary metabolites from Nigella sativa seeds.

    PubMed

    Liu, X; Abd El-Aty, A M; Shim, J-H

    2011-10-01

    Nigella sativa L. (black cumin), commonly known as black seed, is a member of the Ranunculaceae family. This seed is used as a natural remedy in many Middle Eastern and Far Eastern countries. Extracts prepared from N. sativa have, for centuries, been used for medical purposes. Thus far, the organic compounds in N. sativa, including alkaloids, steroids, carbohydrates, flavonoids, fatty acids, etc. have been fairly well characterized. Herein, we summarize some new extraction techniques, including microwave assisted extraction (MAE) and supercritical extraction techniques (SFE), in addition to the classical method of hydrodistillation (HD), which have been employed for isolation and various analytical techniques used for the identification of secondary metabolites in black seed. We believe that some compounds contained in N. sativa remain to be identified, and that high-throughput screening could help to identify new compounds. A study addressing environmentally-friendly techniques that have minimal or no environmental effects is currently underway in our laboratory.

  4. Antioxidant potential of commercially available cumin (Cuminum cyminuml inn) in Pakistan.

    PubMed

    Bukhari, S Birjees; Iqbal, Shahid; Bhanger, M I

    2009-05-01

    Owing to increased safety concerns about synthetic antioxidants, exploitation of safer antioxidants based on natural origin is the focus of research nowadays. Cumin is a common spice and is used as a routine supportive cooking agent. Extracts of cumin were prepared in methanol, ethanol, dichloromethane and hexane by employing Soxhlet extraction apparatus. Determination of the total phenolic content, chelating activity, reducing power and free radical scavenging activity were taken as parameters for the assessment of antioxidant properties. The findings of this study suggest cumin to be a potent source of antioxidants. Results from the different parameters were in agreement with one another.

  5. Protective effect of thymoquinone against diazinon-induced hematotoxicity, genotoxicity and immunotoxicity in rats.

    PubMed

    Danaei, Gholam Hassan; Karami, Mohammad

    2017-10-01

    Several studies have shown that oxidative stress and cell damage can occur in the very early stages of diazinon (DZN) exposure. The present study was designed to determine the beneficial effect of thymoquinone (Thy), the main component of Nigella sativa (black seed or black cumin) against DZN immunotoxicity, hematotoxicity and genotoxicity in rats. In the present experimental study, 48 male Wistar rats were randomly divided into six groups, (eight per group) as follows: control (receiving corn oil as the DZN solvent), DZN (20mg/kg), Thy (10mg/kg), Thy (2.5mg/kg)+DZN, Thy (5mg/kg)+DZN and Thy (10mg/kg)+DZN. After four weeks of treatment, the hematological parameters of red blood cells (RBCs), white blood cells (WBCs), hemoglobin (Hb), hematocrit (Hct) and platelets (PLTs) were evaluated. The evaluation of genotoxicity was carried out using the micronucleus assay. For measurement of cytokine production, interferon gamma (IFN-γ), interleukin 10 (IL10) and interleukin 4 (IL4) were chosen as immunotoxicity indicators of DZN toxicity. DZN was found to decrease RBCs, WBCs, Hb, Hct, PLTs, butyrl- and acetyl-cholinesterase activity and I FN-γ and increased the micronucleus indices of IL10 and IL4 as compared with the control group. Treatment with Thy reduced DZN hematotoxicity and immunotoxicity, but, significantly, did not prevent genotoxicity. This study showed that Thy (without the significant effect on genotoxicity) decreased the hematological toxicity, immunotoxicity and butyrl and acetyl cholinesterase activity induced by DZN. The success of Thy supplementation against DZN toxicity can be attributed to the antioxidant effects of its constituents. Copyright © 2017. Published by Elsevier B.V.

  6. Protective Effects of Essential Oils as Natural Antioxidants against Hepatotoxicity Induced by Cyclophosphamide in Mice

    PubMed Central

    Sheweita, Salah A.; El-Hosseiny, Lobna S.; Nashashibi, Munther A.

    2016-01-01

    Clinical application of cyclophosphamide (CP) as an anticancer drug is often limited due to its toxicity. CP is metabolized mainly in the liver by cytochrome P450 system into acrolein which is the proximate toxic metabolite. Many different natural antioxidants were found to alleviate the toxic effects of various toxic agents via different mechanisms. Therefore, the present study aimed at investigating the role of essential oils extracted from fennel, cumin and clove as natural antioxidants in the alleviation of hepatotoxicity induced by CP through assessment of hepatotoxicity biomarkers (AST, ALT, ALP), histopathology of liver tissues as well as other biochemical parameters involved in the metabolism of CP. The data of the present study showed that treatment of male mice with cyclophosphamide (2.5 mg/Kg BW) as repeated dose for 28 consecutive days was found to induce hepatotoxicity through the elevation in the activities of AST, ALT, and ALP. Combined administration of any of these oils with CP to mice partially normalized the altered hepatic biochemical markers caused by CP, whereas administration of fennel, clove or cumin essential oils alone couldn’t change liver function indices. Moreover, CP caused histological changes in livers of mice including swelling and dilation in sinusoidal space, inflammation in portal tract and hepatocytes, as well as, hyperplasia in Kuppfer cells. However, co-administration of any of the essential oils with CP alleviated to some extent the changes caused by CP but not as the normal liver. CP was also found to induce free radical levels (measured as thiobarbituric acid reactive substances) and inhibited the activities of superoxide dismutase, glutathione reductase, and catalase as well as activities and protein expressions of both glutathione S-transferase (GSTπ) and glutathione peroxidase. Essential oils restored changes in activities of antioxidant enzymes (SOD, CAT, GR, GST, and GPx) caused by CP to their normal levels compared to control group. In addition, treatment of mice with CP was found to induce the protein expression of CYP 3A4, 2B1/2, 2C6, 2C23. Moreover, the present study showed that essential oils reduced the expression of CYPs 2E1, 3A4 but could not restore the expression of CYP 2C6 and 2C23 compared to CP-treated mice. Interestingly, pretreatment of mice with essential oil of clove was found to restore activities of DMN-dI, AHH, and ECOD which were induced by CP to their normal control levels. It is concluded that EOs showed a marked hepatoprotective effect against hepatotoxicity induced by CP. In addition, co-administration of CP with any of these oils might be used as a new strategy for cancer treatment to alleviate the hepatotoxicity induced by CP. PMID:27802299

  7. Protective Effects of Essential Oils as Natural Antioxidants against Hepatotoxicity Induced by Cyclophosphamide in Mice.

    PubMed

    Sheweita, Salah A; El-Hosseiny, Lobna S; Nashashibi, Munther A

    2016-01-01

    Clinical application of cyclophosphamide (CP) as an anticancer drug is often limited due to its toxicity. CP is metabolized mainly in the liver by cytochrome P450 system into acrolein which is the proximate toxic metabolite. Many different natural antioxidants were found to alleviate the toxic effects of various toxic agents via different mechanisms. Therefore, the present study aimed at investigating the role of essential oils extracted from fennel, cumin and clove as natural antioxidants in the alleviation of hepatotoxicity induced by CP through assessment of hepatotoxicity biomarkers (AST, ALT, ALP), histopathology of liver tissues as well as other biochemical parameters involved in the metabolism of CP. The data of the present study showed that treatment of male mice with cyclophosphamide (2.5 mg/Kg BW) as repeated dose for 28 consecutive days was found to induce hepatotoxicity through the elevation in the activities of AST, ALT, and ALP. Combined administration of any of these oils with CP to mice partially normalized the altered hepatic biochemical markers caused by CP, whereas administration of fennel, clove or cumin essential oils alone couldn't change liver function indices. Moreover, CP caused histological changes in livers of mice including swelling and dilation in sinusoidal space, inflammation in portal tract and hepatocytes, as well as, hyperplasia in Kuppfer cells. However, co-administration of any of the essential oils with CP alleviated to some extent the changes caused by CP but not as the normal liver. CP was also found to induce free radical levels (measured as thiobarbituric acid reactive substances) and inhibited the activities of superoxide dismutase, glutathione reductase, and catalase as well as activities and protein expressions of both glutathione S-transferase (GSTπ) and glutathione peroxidase. Essential oils restored changes in activities of antioxidant enzymes (SOD, CAT, GR, GST, and GPx) caused by CP to their normal levels compared to control group. In addition, treatment of mice with CP was found to induce the protein expression of CYP 3A4, 2B1/2, 2C6, 2C23. Moreover, the present study showed that essential oils reduced the expression of CYPs 2E1, 3A4 but could not restore the expression of CYP 2C6 and 2C23 compared to CP-treated mice. Interestingly, pretreatment of mice with essential oil of clove was found to restore activities of DMN-dI, AHH, and ECOD which were induced by CP to their normal control levels. It is concluded that EOs showed a marked hepatoprotective effect against hepatotoxicity induced by CP. In addition, co-administration of CP with any of these oils might be used as a new strategy for cancer treatment to alleviate the hepatotoxicity induced by CP.

  8. Effect of black cumin seeds on growth performance, nutrient utilization, immunity, gut health and nitrogen excretion in broiler chickens.

    PubMed

    Kumar, Pawan; Patra, Amlan Kumar; Mandal, Guru Prasad; Samanta, Indranil; Pradhan, Saktipada

    2017-08-01

    Use of antibiotic growth promoters (AB) as feed additives in broiler chickens poses risks due to cross-resistance amongst pathogens and residues in tissues. The aim of this study was to evaluate the effect of dietary supplementation of black cumin seeds (BCS) as a natural growth promoter in chickens on nutrient utilization, intestinal microbiota and morphology, immunity, antioxidant status, protein deposition in muscles and nitrogen excretion. Broiler chickens were fed BCS at 0, 5, 10 and 20 g kg -1 diet. Body weight gain tended to increase (P = 0.10) and daily feed intake increased quadratically with increasing concentrations of BCS in the diets. Supplementation of BCS resulted in a tendency to decrease feed conversion efficiency on days 28-42. Metabolizability of nutrients increased linearly with increasing doses of BCS. Protein deposition in thigh and breast muscles was increased and nitrogen excretion was reduced by BCS and AB compared with the control (CON). Intestinal morphology in the duodenum, jejunum and ileum was not generally affected by BCS. Counts of total bacteria, Escherichia coli, Lactobacillus spp. and Clostridium spp. were not affected by BCS and AB compared with CON, but Salmonella spp. decreased linearly (P = 0.05) with increasing doses of BCS. Antibody titers against Newcastle disease virus on day 35 increased quadratically (P < 0.001) with increasing doses of BCS. Concentrations of glucose and triglyceride in blood were not affected by BCS. Concentrations of cholesterol decreased linearly while the concentration of total protein increased linearly with increasing doses of BCS. The use of dietary BCS may improve growth performance, immunity and nutrient utilization in broiler chickens. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  9. Black Zira essential oil: Chemical compositions and antimicrobial activity against the growth of some pathogenic strain causing infection.

    PubMed

    Noshad, Mohammad; Hojjati, Mohammad; Alizadeh Behbahani, Behrooz

    2018-03-01

    The aim of this study was to perform chemical compositions and phytochemical analysis of Black Zira essential oil and other goal of this research was to investigate the antimicrobial effects of Black Zira essential oil against Enterobacter aerogenes, Pseudomonas aeruginosa, Escherichia coli, Shigella flexneri, Staphylococcus epidermidis, Streptococcus pyogenes and Candida albicans. Black Zira essential oil was extracted by hydrodistillation method using clevenger apparatus. Black Zira essential oil chemical composition was identified through gas chromatography/mass spectrometry. γ-terpinene with a percentage of 24.8% was the major compound of Black Zira essential oil. The antimicrobial effect Black Zira essential oil was evaluated by several qualitative and quantitative methods (disk diffusion, well diffusion, microdilution broth, agar dilution and minimum bactericidal/fungicidal concentration). Phytochemical analysis Black Zira essential oil were appraised based on qualitative methods. Antioxidant activity (2,2-diphenyl-1-picrylhydrazyl and β-carotene/linoleic acid inhibition) and total phenolic content (Folin-Ciocalteu) were examined. The results of phytochemical analysis of Black Zira essential oil showed the existence of phenolic, flavonoids, saponins, alkaloids and tannins. The total phenolic content and antioxidant activity (reported as IC 50 ) of Black Zira essential oil were equal to 120.50 ± 0.50 mg GAE/g and 11.55 ± 0.25 μg/ml, respectively. The MIC of the Black Zira essential oil ranged from 1 mg/ml to 8 mg/ml, while its MBC and MFC ranged from 1 mg/ml to 16 mg/ml. The results presented that the longest and the shortest inhibition zone diameter at the concentration of 8 mg/ml pertained to C. albicans and E. aerogenes, respectively. Copyright © 2018. Published by Elsevier Ltd.

  10. Spices for Prevention and Treatment of Cancers

    PubMed Central

    Zheng, Jie; Zhou, Yue; Li, Ya; Xu, Dong-Ping; Li, Sha; Li, Hua-Bin

    2016-01-01

    Spices have been widely used as food flavorings and folk medicines for thousands of years. Numerous studies have documented the antioxidant, anti-inflammatory and immunomodulatory effects of spices, which might be related to prevention and treatment of several cancers, including lung, liver, breast, stomach, colorectum, cervix, and prostate cancers. Several spices are potential sources for prevention and treatment of cancers, such as Curcuma longa (tumeric), Nigella sativa (black cumin), Zingiber officinale (ginger), Allium sativum (garlic), Crocus sativus (saffron), Piper nigrum (black pepper) and Capsicum annum (chili pepper), which contained several important bioactive compounds, such as curcumin, thymoquinone, piperine and capsaicin. The main mechanisms of action include inducing apoptosis, inhibiting proliferation, migration and invasion of tumors, and sensitizing tumors to radiotherapy and chemotherapy. This review summarized recent studies on some spices for prevention and treatment of cancers, and special attention was paid to bioactive components and mechanisms of action. PMID:27529277

  11. Spices for Prevention and Treatment of Cancers.

    PubMed

    Zheng, Jie; Zhou, Yue; Li, Ya; Xu, Dong-Ping; Li, Sha; Li, Hua-Bin

    2016-08-12

    Spices have been widely used as food flavorings and folk medicines for thousands of years. Numerous studies have documented the antioxidant, anti-inflammatory and immunomodulatory effects of spices, which might be related to prevention and treatment of several cancers, including lung, liver, breast, stomach, colorectum, cervix, and prostate cancers. Several spices are potential sources for prevention and treatment of cancers, such as Curcuma longa (tumeric), Nigella sativa (black cumin), Zingiber officinale (ginger), Allium sativum (garlic), Crocus sativus (saffron), Piper nigrum (black pepper) and Capsicum annum (chili pepper), which contained several important bioactive compounds, such as curcumin, thymoquinone, piperine and capsaicin. The main mechanisms of action include inducing apoptosis, inhibiting proliferation, migration and invasion of tumors, and sensitizing tumors to radiotherapy and chemotherapy. This review summarized recent studies on some spices for prevention and treatment of cancers, and special attention was paid to bioactive components and mechanisms of action.

  12. In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance

    PubMed Central

    Pandey, Sonika; Patel, Manish Kumar; Jha, Bhavanath

    2016-01-01

    Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13), overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid), and lower electrolytic leakage, lipid peroxidation (MDA content) and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas. PMID:27411057

  13. In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.

    PubMed

    Pandey, Sonika; Patel, Manish Kumar; Mishra, Avinash; Jha, Bhavanath

    2016-01-01

    Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13), overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid), and lower electrolytic leakage, lipid peroxidation (MDA content) and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas.

  14. Breast Cancer-Targeted Nuclear Drug Delivery Overcoming Drug Resistance for Breast Cancer Chemotherapy

    DTIC Science & Technology

    2010-09-01

    compared with the reported results that the cur- cumin concentrations in tumor tissues were usu- ally very low when curcumin was administrated. The...prodrug and as a drug carrier. This cur- cumin prodrug has a fixed composition with a curcumin-loading content of 25.3 wt% and can release curcumin...200 or 400, Aldrich, 99%) was dried over calcium hydride. Cur- cumin (high purity, Axxora LLC) was further purified by repeated recrystallization in

  15. Thymoquinone Attenuates Brain Injury via an Anti-oxidative Pathway in a Status Epilepticus Rat Model.

    PubMed

    Shao, Yi-Ye; Li, Bing; Huang, Yong-Mei; Luo, Qiong; Xie, Yang-Mei; Chen, Ying-Hui

    2017-01-01

    Status epilepticus (SE) results in the generation of reactive oxygen species (ROS), which contribute to seizure-induced brain injury. It is well known that oxidative stress plays a pivotal role in status epilepticus (SE). Thymoquinone (TQ) is a bioactive monomer extracted from black cumin (Nigella sativa) seed oil that has anti-inflammatory, anti-cancer, and antioxidant activity in various diseases. This study evaluated the protective effects of TQ on brain injury in a lithium-pilocarpine rat model of SE and investigated the underlying mechanism related to antioxidative pathway. Electroencephalogram and Racine scale were used to value seizure severity. Passive-avoidance test was used to determine learning and memory function. Moreover, anti-oxidative activity of TQ was observed using Western blot and super oxide dismutase (SOD) activity assay. Latency to SE increased in the TQ-pretreated group compared with rats in the model group, while the total power was significantly lower. Seizure severity measured on the Racine scale was significantly lower in the TQ group compared with the model group. Results of behavioral experiments suggest that TQ may also have a protective effect on learning and memory function. Investigation of the protective mechanism of TQ showed that TQ-pretreatment significantly increased the expression of Nrf2, HO-1 proteins and SOD in the hippocampus. These findings showed that TQ attenuated brain injury induced by SE via an anti-oxidative pathway.

  16. Thymoquinone, as an anticancer molecule: from basic research to clinical investigation

    PubMed Central

    Asaduzzaman Khan, Md.; Tania, Mousumi; Fu, Shangyi; Fu, Junjiang

    2017-01-01

    Thymoquinone is an anticancer phytochemical commonly found in black cumin. In this review, we discuss the potential of thymoquinone as anticancer molecule, its mechanism of action and future usage in clinical applications. Thymoquinone exhibits anticancer activity via numerous mechanisms of action, specifically by showing selective antioxidant and oxidant activity, interfering with DNA structure, affecting carcinogenic signaling molecules/pathways and immunomodulation. In vitro activity of thymoquinone has been further implicated in animal models of cancer; however, no clinical application has been proven yet. This is the optimum time to focus on clinical trials for developing thymoquinone as a future drug in cancer therapeutics. PMID:28881699

  17. Thymoquinone, as an anticancer molecule: from basic research to clinical investigation.

    PubMed

    Asaduzzaman Khan, Md; Tania, Mousumi; Fu, Shangyi; Fu, Junjiang

    2017-08-01

    Thymoquinone is an anticancer phytochemical commonly found in black cumin. In this review, we discuss the potential of thymoquinone as anticancer molecule, its mechanism of action and future usage in clinical applications. Thymoquinone exhibits anticancer activity via numerous mechanisms of action, specifically by showing selective antioxidant and oxidant activity, interfering with DNA structure, affecting carcinogenic signaling molecules/pathways and immunomodulation. In vitro activity of thymoquinone has been further implicated in animal models of cancer; however, no clinical application has been proven yet. This is the optimum time to focus on clinical trials for developing thymoquinone as a future drug in cancer therapeutics.

  18. Development and validation of a duplex real-time PCR assay for the simultaneous detection of three mustard species (Sinapis alba, Brassica nigra and Brassica juncea) in food.

    PubMed

    Palle-Reisch, Monika; Cichna-Markl, Margit; Hochegger, Rupert

    2014-06-15

    The paper presents a duplex real-time PCR assay for the simultaneous detection of three potentially allergenic mustard species commonly used in food: white mustard (Sinapis alba), black mustard (Brassica nigra) and brown mustard (Brassica juncea). White mustard is detected in the "green" and black/brown mustard in the "yellow" channel. The duplex real-time PCR assay does not show cross-reactivity with other Brassicaceae species including broccoli, cauliflower, radish and rapeseed. Low cross-reactivities (difference in the Ct value ⩾ 11.91 compared with the positive control) were obtained with cumin, fenugreek, ginger, rye and turmeric. When applying 500 ng DNA per PCR tube, the duplex real-time PCR assay allowed the detection of white, black and brown mustard in brewed model sausages down to a concentration of 5mg/kg in 10 out of 10 replicates. The duplex real-time PCR assay was applied to verify correct labelling of commercial foodstuffs. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Chemical Compositions, Somatic Embryogenesis, and Somaclonal Variation in Cumin

    PubMed Central

    Tohidfar, Masoud; Sadat Noori, Seyed Ahmad; Izadi Darbandi, Ali; Rao, Rosa

    2017-01-01

    This is the first report evaluating the relationship between the chemical compositions of cumin seeds (based on the analysis of the content of catalase, ascorbate peroxidase, proline, protein, terpenic compounds, alcohol/phenols, aldehydes, and epoxides) and the induction efficiency of somatic embryogenesis in two Iranian superior cumin landraces (Golestan and North Khorasan). Cotyledons isolated from Golestan landrace seeds cultivated on MS medium supplemented with 0.1 mg/L kinetin proved to be the best primary explant for the induction of somatic embryogenesis as well as the regeneration of the whole plantlet. Results indicated that different developmental stages of somatic embryos were simultaneously observed on a callus with embryogenic potential. The high content of catalase, ascorbate peroxidase, proline, and terpenic hydrocarbons and low content of alcoholic and phenolic compositions had a stimulatory effect on somatic embryogenesis. Band patterns of RAPD markers in regenerated plants were different from those of the mother plants. This may be related to somaclonal variations or pollination system of cumin. Generally, measurement of chemical compositions can be used as a marker for evaluating the occurrence of somatic embryogenesis in cumin. Also, somaclonal variations of regenerated plants can be applied by the plant breeders in breeding programs. PMID:29234682

  20. Reservoir heterogeneity in Carboniferous sandstone of the Black Warrior basin. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kugler, R.L.; Pashin, J.C.; Carroll, R.E.

    1994-04-01

    Although oil production in the Black Warrior basin of Alabama is declining, additional oil may be produced through improved recovery strategies, such as waterflooding, chemical injection, strategic well placement, and infill drilling. High-quality characterization of reservoirs in the Black Warrior basin is necessary to utilize advanced technology to recover additional oil and to avoid premature abandonment of fields. This report documents controls on the distribution and producibility of oil from heterogeneous Carboniferous reservoirs in the Black Warrior basin of Alabama. The first part of the report summarizes the structural and depositional evolution of the Black Warrior basin and establishes themore » geochemical characteristics of hydrocarbon source rocks and oil in the basin. This second part characterizes facies heterogeneity and petrologic and petrophysical properties of Carter and Millerella sandstone reservoirs. This is followed by a summary of oil production in the Black Warrior basin and an evaluation of seven improved-recovery projects in Alabama. In the final part, controls on the producibility of oil from sandstone reservoirs are discussed in terms of a scale-dependent heterogeneity classification.« less

  1. Quantitative Scrutinization of Aflatoxins in Different Spices from Pakistan

    PubMed Central

    Kashif, Aiza; Kanwal, Kinza; Khan, Abdul Muqeet; Abbas, Mateen

    2016-01-01

    The current research work aimed to access the contamination level of aflatoxins B1, B2, G1, and G2 in the household spices that are widely consumed in huge amounts. 200 different spice samples, 100 packed and 100 unpacked, were analyzed for the aflatoxins profile by HPLC with an incidence of 61.5% contamination out of which 53.66% samples exceed the EU limit. The results disclosed that the unpacked samples are more contaminated as compared to the packed samples except for white cumin seeds. Among packed and unpacked samples of spices, the maximum value of aflatoxins was detected in fennel, that is, 27.93 μg/kg and 67.04 μg/kg, respectively. The lowest concentration of aflatoxin was detected in cinnamon in packed form (0.79 μg/kg) and in the unpacked samples of white cumin seeds which is 1.75 μg/kg. Caraway seeds and coriander in its unpacked form showed positive results whereas black pepper (packed and unpacked) was found free from aflatoxins. This is the first report on the occurrence of aflatoxins in packed and unpacked samples of spices from Pakistan. To ensure safe consumption of spices, there should be constant monitoring of aflatoxin and more studies need to be executed with the intention of preventing mycotoxin accretion in this commodity. PMID:27781067

  2. EVALUATION OF CARBON BLACK SLURRIES AS CLEAN BURNING FUELS

    EPA Science Inventory

    Experiments were performed to examine the pumpability, atomization and combustion characteristics of slurries made of mixtures of carbon black with No. 2 fuel oil and methanol. Carbon black-No. 2 fuel oil and carbon black-methanol slurries, with carbon black contents of up to 50 ...

  3. Effect of black cumin (Nigella sativa) on cadmium-induced oxidative stress in the blood of rats.

    PubMed

    Kanter, Mehmet; Coskun, Omer; Gurel, Ahmet

    2005-12-01

    The protective effect of black cumin (Nigella sativa = NS) on cadmium-induced oxidative stress was studied in rats. The rats were randomly divided into three experimental groups: A (conrol), B (Cd treated), and C (Cd + NS treated), each containing 10 animals. The Cd-treated and Cd + NS-treated groups were injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl in the amount of 2 mL/kg for 30 d, resulting in a dosage of 0.49 mg Cd/kg/d. The control group was injected with only isotonic NaCl (2 mL/kg/d) throughout the experiment (for 30 d). Three days prior to induction of CdCl2, the Cd + NS-treated group received a daily intraperitoneal injection of 0.2 mL/kg NS until the end of the study. Cd treatment increased significantly the malondialdehyde levels in plasma and erythrocyte (p<0.01 and p<0.05, respectively) and also increased significantly the antioxidant levels (superoxide dismutase, glutathione peroxidase, and catalase) (p<0.05) compared to the control group. Cd + NS treatment decreased significantly the elevated malondialdehyde levels in plasma and erythrocyte (p<0.01 and p<0.05, respectively) and also reduced significantly the enhanced antioxidant levels (p<0.05). Cd treatment increased significantly the activity of iron levels (p<0.05) in the plasma compared to the control group. Cd + NS treatment decreased the activity of iron levels (p<0.05) in the plasma compared to the Cd-treated group. In the control group with no treatment, histology of erythrocytes was normal. In the Cd-treated group, there were remarkable membrane destruction and hemolytic changes in erythrocytes. In the Cd + NS-treated group, these changes were less than in the Cd-treated group. Our results show that N. sativa exerts a protective effect against cadmium toxicity.

  4. Oviposition Deterrent and Larvicidal and Pupaecidal Activity of Seven Essential Oils and their Major Components against Culex quinquefasciatus Say (Diptera: Culicidae): Synergism–antagonism Effects

    PubMed Central

    Andrade-Ochoa, Sergio; Sánchez-Aldana, Daniela; Chacón-Vargas, Karla Fabiola; Rivera-Chavira, Blanca E.; Camacho, Alejandro D.; Nogueda-Torres, Benjamín

    2018-01-01

    The larvicidal activity of essential oils cinnamon (Cinnamomum verum J. Presl), Mexican lime (Citrus aurantifolia Swingle) cumin (Cuminum cyminum Linnaeus), clove (Syzygium aromaticum (L.) Merr. & L.M.Perry), laurel (Laurus nobilis Linnaeus), Mexican oregano (Lippia berlandieri Schauer) and anise (Pimpinella anisum Linnaeus)) and their major components are tested against larvae and pupae of Culex quinquefasciatus Say. Third instar larvae and pupae are used for determination of lethality and mortality. Essential oils with more than 90% mortality after a 30-min treatment are evaluated at different time intervals. Of the essential oils tested, anise and Mexican oregano are effective against larvae, with a median lethal concentration (LC50) of 4.7 and 6.5 µg/mL, respectively. Anise essential oil and t-anethole are effective against pupae, with LC50 values of 102 and 48.7 µg/mL, respectively. Oregano essential oil and carvacrol also have relevant activities. A kinetic analysis of the larvicidal activity, the oviposition deterrent effect and assays of the effects of the binary mixtures of chemical components are undertaken. Results show that anethole has synergistic effects with other constituents. This same effect is observed for carvacrol and thymol. Limonene shows antagonistic effect with β-pinene. The high larvicidal and pupaecidal activities of essential oils and its components demonstrate that they can be potential substitutes for chemical compounds used in mosquitoes control programs. PMID:29443951

  5. Mycotoxins in spices and herbs-An update.

    PubMed

    Kabak, Bulent; Dobson, Alan D W

    2017-01-02

    Spices and herbs have been used since ancient times as flavor and aroma enhancers, colorants, preservatives, and traditional medicines. There are more than 30 spices and herbs of global economic and culinary importance. Among the spices, black pepper, capsicums, cumin, cinnamon, nutmeg, ginger, turmeric, saffron, coriander, cloves, dill, mint, thyme, sesame seed, mustard seed, and curry powder are the most popular spices worldwide. In addition to their culinary uses, a number of functional properties of aromatic herbs and spices are also well described in the scientific literature. However, spices and herbs cultivated mainly in tropic and subtropic areas can be exposed to contamination with toxigenic fungi and subsequently mycotoxins. This review provides an overview on the mycotoxin risk in widely consumed spices and aromatic herbs.

  6. Total antioxidant capacity of commonly used fruits, vegetables, herbs and spices of Pakistan.

    PubMed

    Abid, Mobasher Ali; Ashfaq, Muhammad; Sharif, Muhammad Junaid Hassan; Rauf, Khalid; Mahmood, Wajahat; Khan, Ikarmullah; Abbas, Ghulam

    2017-11-01

    The current study was aimed at investigating the total antioxidant activity (TAC) of various fruits, vegetables, herbs and spices habitat in Pakistan. The ferric reducing ability of plasma (FRAP) assay was used to measure the TAC of various extracts (aqueous, ethanolic and aqueous-ethanolic). Following is the potency order for fruits (guava >strawberry >Pomegranate >apple >kinnow >melon >lemon >banana), vegetables (spinach >Cabbage (Purple) >Jalapeno >Radish >Brinjal >Bell Pepper >Lettuce >Carrot >Cabbage (White) >Onion >Potato >Tomato >Cucumber) and herbs/spices (clove >Rosemary >Thyme >Oregano >Cinnamon >Cumin >Kalonji >Paprika >Neem (Flower) >Fennel >Black Cardamom >Turmeric >Coriander >Ginger >Garlic). In conclusion, the guava, spinach and clove provide the best natural dietary option for treatment / prevention of oxidative stress and thus could alleviate several associated ailments.

  7. Rat Plasma Oxidation Status After Nigella Sativa L. Botanical Treatment in CCL(4)-Treated Rats.

    PubMed

    Soleimani, Hengameh; Ranjbar, Akram; Baeeri, Maryam; Mohammadirad, Azadeh; Khorasani, Reza; Yasa, Narguess; Abdollahi, Mohammad

    2008-01-01

    ABSTRACT Nigella sativa Linn. (family Ranunculaceae), commonly known as black cumin, is native to the Mediterranean area and has been used for thousands of years as a health and beauty aid. The present study investigated the protective effects of Nigella sativa (NS) extract (NSE) and oil (NSO) on CCl(4)-induced nitrosative stress and protein oxidation in rat. CCl(4) (0.8 mg/kg) was used as an aid for induction of nitrosative stress. In vitro antioxidant potential was tested in the presence of 2,4-dinitrophenylhyrdazine (DPPH) as an organic nitrogen radical. Doses of 0.2, 0.3, and 1 mg/kg of the NS extract and oil were administered to CCL(4)-treated rats for 10 days. At the end of treatment, blood was taken from rats under anesthesia and plasma was separated. The concentration of nitric oxide (NO), total antioxidant power (TAP), carbonyl molecules (CM) as measure of protein oxidation (PO), tumor necrosis factor-alpha (TNF-alpha), and total thiol molecules (TTM) were measured in plasma. In vitro evaluation of antioxidant effects of NSE and NSO showed that the highest antioxidant activity (80%) was observed with the concentration of 10 and 20 mg/ml, respectively, that were equal to vitamin E (200 mg/ml). Administration of CCL(4) increased plasma PO, NO, TNF-alpha and decreased TAP and TTM. Both NSE and NSO showed significant protection against CCl(4)-induced changes in biochemical parameters, but not dose-dependently. Doses of 0.3 and 1 mg/kg were more effective than doses of 0.2 mg/kg for both NSE and NSO, but dose of 1 mg/kg was the most effective one. The results indicate the potential of NS in preventing CCL(4)-induced toxic nitrosative stress. It is concluded that NS has marked antioxidant potentials that may be beneficial in alleviating complications of many illnesses related to oxidative/nitrosative stress in humans, but preclinical safety measures should be completed before clinical trials.

  8. Plant essential oils and allied volatile fractions as multifunctional additives in meat and fish-based food products: a review.

    PubMed

    Patel, Seema

    2015-01-01

    Essential oils are concentrated aromatic volatile compounds derived from botanicals by distillation or mechanical pressing. They play multiple, crucial roles as antioxidants, food pathogen inhibitors, shelf-life enhancers, texture promoters, organoleptic agents and toxicity-reducing agents. For their versatility, they appear promising as food preservatives. Several research findings in recent times have validated their potential as functional ingredients in meat and fish processing. Among the assortment of bioactive compounds in the essential oils, p-cymene, thymol, eugenol, carvacrol, isothiocyanate, cinnamaldehyde, cuminaldehyde, linalool, 1,8-cineol, α-pinene, α-terpineol, γ-terpinene, citral and methyl chavicol are most familiar. These terpenes (monoterpenes and sesquiterpenes) and phenolics (alcohols, esters, aldehydes and ketones) have been extracted from culinary herbs such as oregano, rosemary, basil, coriander, cumin, cinnamon, mint, sage and lavender as well as from trees such as myrtle, fir and eucalyptus. This review presents essential oils as alternatives to conventional chemical additives. Their synergistic actions with modified air packaging, irradiation, edible films, bacteriocins and plant byproducts are discussed. The decisive roles of metabolic engineering, microwave technology and metabolomics in quality and quantity augmentation of essential oil are briefly mooted. The limitations encountered and strategies to overcome them have been illuminated to pave way for their enhanced popularisation. The literature has been mined from scientific databases such as Pubmed, Pubchem, Scopus and SciFinder.

  9. Black seed oil ameliorates allergic airway inflammation by inhibiting T-cell proliferation in rats.

    PubMed

    Shahzad, Muhammad; Yang, Xudong; Raza Asim, M B; Sun, Qingzhu; Han, Yan; Zhang, Fujun; Cao, Yongxiao; Lu, Shemin

    2009-02-01

    The black seeds, from the Ranunculaceae family, have been traditionally used by various cultures as a natural remedy for several ailments. In this study, we examined the effect of black seed oil as an immunomodulator in a rat model of allergic airway inflammation. Rats sensitized to ovalbumin and challenged intranasally with ovalbumin to induce an allergic inflammatory response were compared to ovalbumin-sensitized, intranasally ovalbumin-exposed rats pretreated with intraperitoneally administered black seed oil and to control rats. The levels of IgE, IgG1 and ova-specific T-cell proliferation in spleen were measured by ELISA. The pro-inflammatory cytokine IL-4, IL-5, IL-6 and TGF-beta1 mRNA expression levels were measured by reverse transcription polymerase chain reaction. The intraperitoneal administration of black seed oil inhibited the Th2 type immune response in rats by preventing inflammatory cell infiltration and pathological lesions in the lungs. It significantly decreased the nitric oxide production in BALF, total serum IgE, IgG1 and OVA-specific IgG1 along with IL-4, IL-5, IL-6 and TGF-beta1 mRNA expression. Black seed oil treatment resulted in decreased T-cell response evident by lesser delayed type hypersensitivity and lower T-cell proliferation in spleen. In conclusion, black seed oil exhibited a significant reduction in all the markers of allergic inflammation mainly by inhibiting the delayed type hypersensitivity and T-cell proliferation. The data suggests that inhibition of T-cell response may be responsible for immunomodulatory effect of black seed oil in the rat model of allergic airway inflammation.

  10. New tool for the Black Sea environmental safety: BlackSea Track Web

    NASA Astrophysics Data System (ADS)

    Kubryakov, A. I.; Korotayev, G. K.; Thoorens, F.-X.; Liungman, O.; Ambjorn, C.

    2012-04-01

    Huge increases in the volume of oil being transported across the Black Sea have greatly increased the risk of oil pollution. Remote sensing data show that the majority of oil spills occur along major shipping routes, suggesting that shipping, rather than land-based oil installations have been the principal cause of concern. However, a single large spill from ships, platforms or land-based oil installations could severely impact biota and the economies of all coastal countries and could produce significant damage of the Black Sea ecosystem and fishing. Also, due to the semi-enclosed character of the basin, oil spill will definitely pollute coast of the basin suffering great losses to the recreation industry and potentially to the human health. Fighting oil pollution in the Black Sea is a great challenge. The challenge is likely to become even greater in the future as maritime traffic is expected to increase over the next few years, making offences and accidents more likely. The risks of shipwrecks and catastrophic oil spill necessitate the use of the modern technologies to effectively protect the marine environment. In turn, such technologies require high-quality products of operational oceanography. Recently such products are the products of the MyOcean project. The development of the Black Sea operational oceanography made it possible to transfer of cost-efficient technologies to the region to create a new tool against oil pollution and for life-saving in case of a shipwreck. The Black Sea Track Web (BSTW) system of accidental oil spill evolution forecast in the Black Sea has been created in the framework of MONINFO project. The system is based on the Seatrack Web (STW) model developing by the consortium of the Baltic Sea countries. It is adapted to the configuration of the Black Sea observing system and is implemented to the regional contingency plans. The BSTW system consists of three parts: - forcing in the form of forecasted stratification, sea currents and wind fields, which is provided by the Black Sea MFC located at MHI in Sevastopol. The Black Sea MFC is the MyOcean regional marine forecasting center. It runs operationally and forms weather and ocean forecasts; - the oil drift model jointly developed by SMHI and the Royal Danish Administration of Navigation and Hydrography and which takes into account and adequately describes almost all physical processes affecting the oil spill; - the graphical user interface developed by SMHI and based on open source GIS-server technology. The developed BSTW system is available via the Internet, fully operational 24 hours a day and user friendly. It allows immediate access to the latest forecasts that drives the system. And in addition, it provides other floating objects and back tracking.

  11. Characterization of volatile aroma compounds from red and black rice bran.

    PubMed

    Sukhonthara, Sukhontha; Theerakulkait, Chockchai; Miyazawa, Mitsuo

    2009-01-01

    The volatile oils from red and black rice bran were obtained by hydrodistillation using diethyl ester and the components of that oil were analyzed by capillary GC-MS. The volatile components of essential oil from red and black rice bran were analyzed by GC and GC-MS. One hundred twenty-nine (129) of volatile compounds were identified in red and black rice bran. Myristic acid, nonanal, (E)-beta-ocimene and 6, 10, 14-trimethyl-2-pentadecanone were main compounds in red rice bran, whereas myristic acid, nonanal, caproic acid, pentadecanal and pelargonic acid were main compounds in black rice bran. Guaiacol, presented at 0.81 mg/100 g in black rice bran, is responsible for the characteristic component in black rice.

  12. Additive impacts on particle emissions from heating low emitting cooking oils

    NASA Astrophysics Data System (ADS)

    Amouei Torkmahalleh, M.; Zhao, Y.; Hopke, P. K.; Rossner, A.; Ferro, A. R.

    2013-08-01

    The effect of five additives, including table salt, sea salt, black pepper, garlic powder, and turmeric, on the emission of PM2.5 and ultrafine particles (UFP) from heated cooking oil (200 °C) were studied. One hundred milligrams of the additives were added individually to either canola or soybean oil without stirring. Black pepper, table salt, and sea salt reduced the PM2.5 emission of canola oil by 86% (p < 0.001), 88% (p < 0.001), and 91% (p < 0.001), respectively. Black pepper, table salt, and sea salt also decreased the total particle number emissions of canola oil by 45% (p = 0.003), 52% (p = 0.001), and 53% (p < 0.001), respectively. Turmeric and garlic powder showed no changes in the PM2.5 and total number emissions of canola oil. Table salt and sea salt, decreased the level of PM2.5 emissions from soybean oil by 47% (p < 0.001) and 77% (p < 0.001), respectively. No differences in the PM2.5 emissions were observed when other additives were added to soybean oil. Black pepper, sea salt, and table salt reduced the total particle number emissions from the soybean oil by 51%, 61% and 68% (p < 0.001), respectively. Turmeric and garlic powder had no effect on soybean oil with respect to total particle number emissions. Our results indicate that table salt, sea salt, and black pepper can be used to reduce the particle total number and PM2.5 emissions when cooking with oil.

  13. No. 2 fuel oil decreases embryonic survival of great black-backed gulls

    USGS Publications Warehouse

    Coon, Nancy C.; Albers, Peter H.; Szaro, Robert C.

    1979-01-01

    The great black-backed gull (Larus marinus) is widespread in the northern hemisphere, breeding south to Britain and Ireland on the European side of the Atlantic and to Long Island in the United States where populations have increased markedly during the last 50 years (DRURY 1979). With growing exploitation of oil resources, seabird populations are being increasingly threatened by accidental oiling of individuals and the subsequent contamination of their eggs and young. It is generally agreed that gulls and terns, which spend much of their time airborne, are less vulnerable to oil pollution than alcids and seaducks (BOURNE 1968, VERMEER AND ANWEILER 1975). Nevertheless, oiled great black-backed gulls were sighted after the Argo Merchant spill off Nantucket Island in December 1976, demonstrating that this species of gull can be affected by surface oil (GROSE AND MATTSON 1977). In this paper we wish to report results of two concurrent studies in which eggs of the great black-backed gull were externally contaminated with No. 2 fuel oil.

  14. Nesting biology of Black-shouldered Kite (Elanus caeruleus) in oil palm landscape in Carey Island, Peninsular Malaysia.

    PubMed

    Ramli, Rosli; Fauzi, Aimi

    2018-03-01

    Black-shouldered Kite ( Elanus caeruleus ) is a well-known raptor that inhabits open areas such as oil palm plantation or paddy field. To determine preferable habitat and nesting site of Black-shouldered Kite in oil palm landscape, we conducted a study on Black-shouldered Kite's nesting biology in Carey Island, Selangor, Malaysia. We divided the island into six types of habitat and conducted road-side count of Black-shouldered Kite from April 2009 to February 2011. Whenever the Black-shouldered Kite was detected, we thoroughly searched the surrounding area for their nest. In total, we have recorded forty nests. The nests were built on 15 species of trees but most of the trees shared common physical characteristics. Some novice breeders also used oil palm tree as their nesting site. Structure and building materials of nests constructed on oil palm trees were different from nests built on other trees. Of all breeding attempts, only four nests which were located in residential area adjacent to young oil palm habitat were successful. Among important characteristics of successful nesting site include taller trees with strong branches and good leaf coverage. These trees not only protect nests from predator detection (except from other predatory birds) and physical environment but also facilitate Black-shouldered Kite's foraging activities by providing good vantage point.

  15. Anti-biofilm, anti-hemolysis, and anti-virulence activities of black pepper, cananga, myrrh oils, and nerolidol against Staphylococcus aureus.

    PubMed

    Lee, Kayeon; Lee, Jin-Hyung; Kim, Soon-Il; Cho, Moo Hwan; Lee, Jintae

    2014-11-01

    The long-term usage of antibiotics has resulted in the evolution of multidrug-resistant bacteria. Unlike antibiotics, anti-virulence approaches target bacterial virulence without affecting cell viability, which may be less prone to develop drug resistance. Staphylococcus aureus is a major human pathogen that produces diverse virulence factors, such as α-toxin, which is hemolytic. Also, biofilm formation of S. aureus is one of the mechanisms of its drug resistance. In this study, anti-biofilm screening of 83 essential oils showed that black pepper, cananga, and myrrh oils and their common constituent cis-nerolidol at 0.01 % markedly inhibited S. aureus biofilm formation. Furthermore, the three essential oils and cis-nerolidol at below 0.005 % almost abolished the hemolytic activity of S. aureus. Transcriptional analyses showed that black pepper oil down-regulated the expressions of the α-toxin gene (hla), the nuclease genes, and the regulatory genes. In addition, black pepper, cananga, and myrrh oils and cis-nerolidol attenuated S. aureus virulence in the nematode Caenorhabditis elegans. This study is one of the most extensive on anti-virulence screening using diverse essential oils and provides comprehensive data on the subject. This finding implies other beneficial effects of essential oils and suggests that black pepper, cananga, and myrrh oils have potential use as anti-virulence strategies against persistent S. aureus infections.

  16. Effects of Cuminum cyminum L. essential oil on some epididymal sperm parameters and histopathology of testes following experimentally induced copper poisoning in mice.

    PubMed

    Sakhaee, E; Emadi, L; Azari, O; Kheirandish, R; Esmaili Nejad, M R; Shafiei Bafti, H

    2016-06-01

    Copper overload can cause sperm cell damage by inducing oxidative stress. On the other hand, cumin has a good antioxidant potential. Therefore, the aim of this study was to evaluate the effects of cumin on sperm quality and testicular tissue following experimentally induced copper poisoning in mice. Forty-eight mature male mice were divided into four equal groups as follows: group Cu which received 0.1 ml copper sulphate at dose of 100 mg kg(-1) , group Cc which received Cuminum cyminum at dose of 1 mg kg(-1) , treatment group which received copper sulphate (100 mg kg(-1) ) and treated with Cuminum cyminum (1 mg kg(-1) ), and control group which received the same volume of normal saline. Six mice in each group were sacrificed at week 4 and week 6. The results showed that sperm concentration, motility and viability in group Cu were significantly decreased at weeks 4 and 6, and severe degenerative changes were observed in testicular tissues in comparison with the control group. In treatment group, significant improvement in the sperm count, motility and viability, and normal architecture in most seminiferous tubules with organised epithelium was observed compared to the group Cu. The sperm quality parameters in the treatment group approached those of the control group. © 2015 Blackwell Verlag GmbH.

  17. Thermoregulation in a naturally oil-contaminated Black-billed Murre Uria aalge

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jenssen, B.M.; Ekker, M.; Bech, C.

    1985-07-01

    The effect of oiled plumages on seabirds is considered to be reduced thermal insulation, with a resultant increase in metabolic rate. This conclusion is based on results from ducks with experimentally oil-contaminated plumages. In the present paper, data on body temperature, metabolic rate, and insulation in a Black-billed Murre (Uria aalge) contaminated during an oil-spill at sea are presented.

  18. What Is Acne?

    MedlinePlus

    ... skin’s oil glands and hair follicles. The small holes in your skin (pores) connect to oil glands ... that rise to the skin’s surface and look black (the black color is not from dirt). Papules: ...

  19. Essential oils to control Botrytis cinerea in vitro and in vivo on plum fruits.

    PubMed

    Aminifard, Mohammad Hossein; Mohammadi, Samane

    2013-01-01

    The consequence of misusing chemical biocides in controlling pests and diseases has drawn the attention of policy makers to the development of methods potentially available in nature for this purpose. In the present study the inhibitory effects of black caraway, fennel and peppermint essential oils against Botrytis cinerea were tested at various concentrations in vitro and in vivo. The in vitro results showed that the growth of B. cinerea was completely inhibited by the application of black caraway and fennel oils at concentrations of 400 and 600 µL L⁻¹ respectively. The in vivo results indicated that black caraway, fennel and peppermint oils at all applied concentrations inhibited B. cinerea growth on plum fruits compared with the control. In addition, all three oils at higher concentrations showed positive effects on fruit quality characteristics such as titrable acidity, total soluble solids, carbohydrate content, pH and weight loss percentage. Thus the oils inhibited the infection of plum fruits by B. cinerea and increased their storage life. This research confirms the antifungal effects of black caraway, fennel and peppermint essential oils both in vitro and in vivo on plum fruits postharvest. Therefore these essential oils could be an alternative to chemicals to control postharvest phytopathogenic fungi on plum fruits. Copyright © 2012 Society of Chemical Industry.

  20. A scalable parallel black oil simulator on distributed memory parallel computers

    NASA Astrophysics Data System (ADS)

    Wang, Kun; Liu, Hui; Chen, Zhangxin

    2015-11-01

    This paper presents our work on developing a parallel black oil simulator for distributed memory computers based on our in-house parallel platform. The parallel simulator is designed to overcome the performance issues of common simulators that are implemented for personal computers and workstations. The finite difference method is applied to discretize the black oil model. In addition, some advanced techniques are employed to strengthen the robustness and parallel scalability of the simulator, including an inexact Newton method, matrix decoupling methods, and algebraic multigrid methods. A new multi-stage preconditioner is proposed to accelerate the solution of linear systems from the Newton methods. Numerical experiments show that our simulator is scalable and efficient, and is capable of simulating extremely large-scale black oil problems with tens of millions of grid blocks using thousands of MPI processes on parallel computers.

  1. Examining food additives and spices for their anti-oxidant ability to counteract oxidative damage due to chronic exposure to free radicals from environmental pollutants

    NASA Astrophysics Data System (ADS)

    Martinez, Raul A., III

    The main objective of this work was to examine food additives and spices (from the Apiaceae family) to determine their antioxidant properties to counteract oxidative stress (damage) caused by Environmental pollutants. Environmental pollutants generate Reactive Oxygen species and Reactive Nitrogen species. Star anise essential oil showed lower antioxidant activity than extracts using DPPH scavenging. Dill Seed -- Anethum Graveolens -the monoterpene components of dill showed to activate the enzyme glutathione-S-transferase , which helped attach the antioxidant molecule glutathione to oxidized molecules that would otherwise do damage in the body. The antioxidant activity of extracts of dill was comparable with ascorbic acid, alpha-tocopherol, and quercetin in in-vitro systems. Black Cumin -- Nigella Sativa: was evaluated the method 1,1-diphenyl2-picrylhhydrazyl (DPPH) radical scavenging activity. Positive correlations were found between the total phenolic content in the black cumin extracts and their antioxidant activities. Caraway -- Carum Carvi: The antioxidant activity was evaluated by the scavenging effects of 1,1'-diphenyl-2-picrylhydrazyl (DPPH). Caraway showed strong antioxidant activity. Cumin -- Cuminum Cyminum - the major polyphenolic were extracted and separated by HPTLC. The antioxidant activity of the cumin extract was tested on 1,1'-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging. Coriander -- Coriandrum Sativum - the antioxidant and free-radical-scavenging property of the seeds was studied and also investigated whether the administration of seeds curtails oxidative stress. Coriander seed powder not only inhibited the process of Peroxidative damage, but also significantly reactivated the antioxidant enzymes and antioxidant levels. The seeds also showed scavenging activity against superoxides and hydroxyl radicals. The total polyphenolic content of the seeds was found to be 12.2 galic acid equivalents (GAE)/g while the total flavonoid content was found to be 12.6 quercetin equivalents/g. The Maximum free radical-scavenging action and free radical reducing power of coriander seed extract was observed at a concentration of 50microg galic acid equivalents (GAE) (Sharma Om P. et al 2009) Anise - Pimpinella Anisum - the seeds of anise were investigated and the extracts showed marked antioxidant activity, both linoleic acid and liposome model systems. The antioxidant activity of alcohol extracts decreased in the order chamomile flowers > gtill seeds>gtnise seeds. The extract also showed good free radical-scavenging 2,2'-diphenyl-l-picrylhydrazyl (DPPH) activity, indicating that they act as hydrogen donors. (Sharma, Om P. et al 2009) Carrot Seed-- Daucus Carota --carrot seed is known for containing potent amounts of antioxidants. It is known for its phenomenal free-radical scavenging and oxygen radical absorbing abilities. It contains high levels of carotenoids and components of vitamin A and powerful antioxidants. It also has high levels of tocopherol, a fat soluble form of vitamin E Carrot seed is a natural antioxidant. Total Phenolic content in extracts was measured by the Folin-Ciocalteu (FC) reagent method. Results were expressed as micrograms of galic acid equivalents (GAE). Celery Seed -- Apium Graveolens - Celery contains well known antioxidants like vitamin C and flavonoids. Scientists have identified at least a dozen other types of antioxidants in celery. Celery is an important food source of conventional antioxidant nutrients, including vitamin C, beta-carotene, and manganese. But its claim to fame may very well be its phytonutrients. Many of these phytonutrients fall into the category of phenolic antioxidants and have been shown to provide anti-inflammatory benefits as well. Celery is an excellent source of vitamin K and molybdenum. It is a very good source of folate, potassium, dietary fiber, manganese, and pantothenic acid. Celery is also a good source of vitamin B2, copper, vitamin C, vitamin A, and vitamin E. The total phenolic content was measured by the Folin-Ciocalteu (FC) reagent method. Fennel--- Foeniculum Vulgare : Like many of its fellow spices contains its own unique combinations of phytonutrients -- including the flavonoids rutin, quercetin, and various kaempferol glycosides -- that give it strong antioxidant activity. The phytonutrients in fennel extracts compare favorably in research studies to BHT (butylated hydroxytoluene), a potentially toxic antioxidant commonly added to processed food. The most fascinating phytonutrients compound in fennel, may be anethole - the primary component of its volatile oil. In addition to its unusual phytonutrients, fennel bulb is an excellent source of vitamin C Vitamin C is the body's primary water-soluble antioxidant, able to neutralize free radicals in all aqueous environments of the body. The vitamin C found in fennel bulb is directly antimicrobial and is also needed for the proper function of the immune system. The total phenolic content was measured by the Folin-Ciocalteu reagent method Cilantro Powder -- Coriandrum Sativum - Cilantro herb is very low in calories and contains no cholesterol. However its deep-green leaves possess good amounts of antioxidants, essential oils, vitamins, and dietary fiber, which help reduce LDL or "bad cholesterol" while raising HDL or "good cholesterol" levels. Its leaves and seeds contain many essential volatile oils. The leaves and stem are also rich in numerous antioxidant polyphenolic Flavonoids such as quercetin, kaempferel, rhamnetin, and epigenin. The herb is a good source of minerals like potassium, calcium manganese, iron, and magnesium. Carrot Powder -- Daucus Carota : Carrot is known for containing potent amounts of antioxidants. It is known for its phenomenal free-radical scavenging and oxygen radical absorbing abilities. It contains high levels of carotenoids and components of vitamin A and powerful antioxidants. It also has high levels of tocopherol, a fat soluble form of vitamin E Carrot seed is a natural antioxidant. Total Phenolic content in extracts was measured by the Folin-Ciocalteu (FC) reagent method. Results were expressed as micrograms of galic acid equivalents (GAE). Weed -- Anethum Graveolens: Dill weed contains numerous plant derived chemical compounds that are known to have been anti-oxidant, disease preventing, and health promoting properties. This popular herb contains no cholesterol and low in calories. Nonetheless, it holds many antioxidants, vitamins like niacin, pyridoxine, etc. and dietary fibers. Dill seeds contain many essential volatile oils such as d-carvone, clillapiol, DHC, eugenol, limonene, terpinene, and myristicin. Dill is also rich in many vital vitamins, including folic acid, riboflavin, vitamin A, 13-carotene, vitamin C, that is essential for optimum metabolism in the human body. Dill weed is a good source of minerals like potassium, calcium manganese, iron, and magnesium. Ammi -- Ammi visnaga L.: Eleven flavonols have been isolated from the aerial parts of Ammi visnaga L. from which four aglycones, four monoglycosides, two diglycosides and one triglycoside. Ammi oil evaluation was done by the antioxidant power of 1,11-diphenyl-2picrylhydrazal (DPPH) radical scavenging activity. DPPH is a stable free radical, which changes its colour from violet to yellow upon reduction by the process of electron donation. The scavenging potential of the antioxidants present in E.O.A seeds can thus be determined by their degree of discoloration to yellow. As per data obtained through experiment, it was seen that scavenging effect of DPPH radical increased with increasing the concentration of essential oil (1.25-10micro1).E.O.A. The E.O.A. has shown considerable reducing power) They exhibited moderate scavenging activity when compared with ascorbic acid (standards). The ability of samples to scavenge DPPH radical was determined on the basis of their concentration providing % effect. Parsley Seed -- Petroselinum Sativum: The flavonoids in parsley -- especially luteolin -- have been shown to function as antioxidants that combine with highly reactive oxygen-containing molecules (oxygen radicals) and help prevent oxidative damage. In addition to its volatile oils and flavonoids, parsley is an excellent source of vitamin C and a good source of vitamin A (notably through its concentration of the pro-vitamin A carotenoids, beta-carotene). Vitamin C has many different functions. It is the body's primary water-soluble antioxidant, rendering harmless dangerous free radicals in all water soluble areas of the body. Beta carotene, another important antioxidant, works in the fat soluble areas of the body. (Bate-Smith et al 1962).

  2. Naturally occurring bioactive compounds from four repellent essential oils against Bemisia tabaci whiteflies.

    PubMed

    Deletre, Emilie; Chandre, Fabrice; Barkman, Barbara; Menut, Chantal; Martin, Thibaud

    2016-01-01

    In tropical countries, netting is an effective sustainable tool for protecting horticultural crops against Lepidoptera, although not against small pests such as Bemisia tabaci, while smaller mesh netting can be used in temperate regions. A solution is to combine a net with a repellent. Previously we identified repellent essential oils: lemongrass (Cymbopogon citratus), cinnamon (Cinnamomum zeylanicum), cumin (Cuminum cyminum) and citronella (Cymbopogon winternarius). The present study was designed to identify the active compounds of these essential oils, characterise their biological activity and examine their potential for coating nets. We investigated the efficiency and toxicity of nets dipped in different solutions. We then studied the repellent effect with an olfactometer and the irritant effect by videotracking. Geraniol and citronellol were the most promising net coatings owing to their repellent effect. The repellency, irritancy or toxicity varied with the product and concentration, and these features were independent, indicating that the repellent and the irritant/toxic mechanisms were not the same. The combined effects of these different compounds account for the bioactivity of the mixture, suggesting interactions between the compounds. This new sustainable strategy for protecting vegetable crops against whiteflies is discussed, in addition to the use of companion plants that could produce such bioactive compounds. © 2015 Society of Chemical Industry.

  3. Effect of two inner-ring oil-flow distribution schemes on the operating characteristics of a 35 millimeter bore ball bearing to 2.5 million DN

    NASA Technical Reports Server (NTRS)

    Schuller, F. T.; Pinel, S. I.; Signer, H. R.

    1985-01-01

    Parametric tests were conducted with a 35-mm-bore, split-inner-ring ball bearing with a double-inner-land-guided cage. Provisions were made for through-the-inner-ring lubrication. Test condictions were either a thrust load of 667 N (150 lb) or a combined load of 667 N (150 lb) thrust and 222 N (50 lb) radial, shaft speeds from 32000 to 72000 rpm, and an oil-inlet temperature of 394 K (250 deg F). Outer ring cooling was used in some tests. Tests were run with either 50 or 75 percent of the total oil flow distributed to the inner-ring raceway. Successful operation was experienced with both 50% and 75% flow patterns to 2.5 million DN. Cooling the outer ring had little effect on inner-ring temperature; however, the outer-ring temperature decreased as much as 7% at 2.5 million DN. Maximum recorded power loss was 3.1 kW (4.2 hp), and maximum cage slip was 8.7 percent. Both occurred at a shaft speed of 72000 rpm, a lubricant flow rate of 1900 cu/min (0.50 gal/min), a combined load, and no outer-ring cooling.

  4. Self-cementing properties of oil shale solid heat carrier retorting residue.

    PubMed

    Talviste, Peeter; Sedman, Annette; Mõtlep, Riho; Kirsimäe, Kalle

    2013-06-01

    Oil shale-type organic-rich sedimentary rocks can be pyrolysed to produce shale oil. The pyrolysis of oil shale using solid heat carrier (SHC) technology is accompanied by large amount of environmentally hazardous solid residue-black ash-which needs to be properly landfilled. Usage of oil shale is growing worldwide, and the employment of large SHC retorts increases the amount of black ash type of waste, but little is known about its physical and chemical properties. The objectives of this research were to study the composition and self-cementing properties of black ash by simulating different disposal strategies in order to find the most appropriate landfilling method. Three disposal methods were simulated in laboratory experiment: hydraulic disposal with and without grain size separation, and dry dumping of moist residue. Black ash exhibited good self-cementing properties with maximum compressive strength values of >6 MPa after 90 days. About 80% of strength was gained in 30 days. However, the coarse fraction (>125 µm) did not exhibit any cementation, thus the hydraulic disposal with grain size separation should be avoided. The study showed that self-cementing properties of black ash are governed by the hydration of secondary calcium silicates (e.g. belite), calcite and hydrocalumite.

  5. First ATRF Chili Contest Proves It’s Not Always about the Meat | Poster

    Cancer.gov

    Wafting cumin through the building, 15 slow cookers full of chili lined an L-shaped conference room at the Frederick National Laboratory for Cancer Research Advanced Technology Research Facility (ATRF), as voters strolled past “No electioneering” signs and the tasting began Feb. 29. Chili No. 13 came out of the gate with a spicy authority. “My tongue’s still tingling,” said one taster. “Just looking at it, you can tell how spicy it is,” said another judge. Thick with ground beef, with a smoky, peppery aroma, it was layered with black beans and corn, and it had a back-of-the-throat burn no other contestant’s offering could match. A nearby box of tissues served as a must-have for anyone sampling the sinus-clearing concoction.

  6. Bactericidal and anti-adhesive properties of culinary and medicinal plants against Helicobacter pylori.

    PubMed

    O'Mahony, Rachel; Al-Khtheeri, Huda; Weerasekera, Deepaka; Fernando, Neluka; Vaira, Dino; Holton, John; Basset, Christelle

    2005-12-21

    To investigate the bactericidal and anti-adhesive properties of 25 plants against Helicobacter pylori (H. pylori). Twenty-five plants were boiled in water to produce aqueous extracts that simulate the effect of cooking. The bactericidal activity of the extracts was assessed by a standard kill-curve with seven strains of H. pylori. The anti-adhesive property was assessed by the inhibition of binding of four strains of FITC-labeled H. pylori to stomach sections. Of all the plants tested, eight plants, including Bengal quince, nightshade, garlic, dill, black pepper, coriander, fenugreek and black tea, were found to have no bactericidal effect on any of the isolates. Columbo weed, long pepper, parsley, tarragon, nutmeg, yellow-berried nightshade, threadstem carpetweed, sage and cinnamon had bactericidal activities against H. pylori, but total inhibition of growth was not achieved in this study. Among the plants that killed H. pylori, turmeric was the most efficient, followed by cumin, ginger, chilli, borage, black caraway, oregano and liquorice. Moreover, extracts of turmeric, borage and parsley were able to inhibit the adhesion of H. pylori strains to the stomach sections. Several plants that were tested in our study had bactericidal and/or anti-adhesive effects on H. pylori. Ingestion of the plants with anti-adhesive properties could therefore provide a potent alternative therapy for H. pylori infection, which overcomes the problem of resistance associated with current antibiotic treatment.

  7. Inhibitory Effect of Black and Red Pepper and Thyme Extracts and Essential Oils on Enterohemorrhagic Escherichia coli and DNase Activity of Staphylococcus aureus.

    PubMed

    Zarringhalam, Maryam; Zaringhalam, Jalal; Shadnoush, Mehdi; Safaeyan, Firouzeh; Tekieh, Elaheh

    2013-01-01

    In this study, extracts and essential oils of Black and Red pepper and Thyme were tested for antibacterial activity against Escherichia coli O157: H7 and Staphylococcus aureus. Black and Red pepper and Thyme were provided from Iranian agricultural researches center. 2 g of each plant powder was added to 10 cc ethanol 96°. After 24 h, the crude extract was separated as an alcoholic extract and concentrated by distillation method. Plants were examined for determining their major component and essential oils were separated. Phytochemical analyses were done for detection of some effective substances in extracts. The antibacterial activity against Escherichia coli O157: H7 and Staphylococcus aureus was tested and the results showed that all extracts and essential oils were effective and essential oils were more active. The extracts and oils that showed antimicrobial activity were later tested to determine the Minimum Inhibitory Dilution (MID) for those bacteria. They were also effective on the inhibition of DNase activity. This study was indicated that extracts and essential oils of Black and Red pepper and Thyme can play a significant role in inhibition of Escherichia coli O157: H7 and Staphylococcus aureus.

  8. Reservoir heterogeneity in Carter Sandstone, North Blowhorn Creek oil unit and vicinity, Black Warrior Basin, Alabama

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kugler, R.L.; Pashin, J.C.

    1992-05-01

    This report presents accomplishments made in completing Task 3 of this project which involves development of criteria for recognizing reservoir heterogeneity in the Black Warrior basin. The report focuses on characterization of the Upper Mississippian Carter sandstone reservoir in North Blowhorn Creek and adjacent oil units in Lamar County, Alabama. This oil unit has produced more than 60 percent of total oil extracted from the Black Warrior basin of Alabama. The Carter sandstone in North Blowhorn Creek oil unit is typical of the most productive Carter oil reservoirs in the Black Warrior basin of Alabama. The first part of themore » report synthesizes data derived from geophysical well logs and cores from North Blowhorn Creek oil unit to develop a depositional model for the Carter sandstone reservoir. The second part of the report describes the detrital and diagenetic character of Carter sandstone utilizing data from petrographic and scanning electron microscopes and the electron microprobe. The third part synthesizes porosity and pore-throat-size-distribution data determined by high-pressure mercury porosimetry and commercial core analyses with results of the sedimentologic and petrographic studies. The final section of the report discusses reservoir heterogeneity within the context of the five-fold classification of Moore and Kugler (1990).« less

  9. Inhibitory Effect of Black and Red Pepper and Thyme Extracts and Essential Oils on Enterohemorrhagic Escherichia coli and DNase Activity of Staphylococcus aureus

    PubMed Central

    Zarringhalam, Maryam; Zaringhalam, Jalal; Shadnoush, Mehdi; Safaeyan, Firouzeh; Tekieh, Elaheh

    2013-01-01

    In this study, extracts and essential oils of Black and Red pepper and Thyme were tested for antibacterial activity against Escherichia coli O157: H7 and Staphylococcus aureus. Black and Red pepper and Thyme were provided from Iranian agricultural researches center. 2 g of each plant powder was added to 10 cc ethanol 96°. After 24 h, the crude extract was separated as an alcoholic extract and concentrated by distillation method. Plants were examined for determining their major component and essential oils were separated. Phytochemical analyses were done for detection of some effective substances in extracts. The antibacterial activity against Escherichia coli O157: H7 and Staphylococcus aureus was tested and the results showed that all extracts and essential oils were effective and essential oils were more active. The extracts and oils that showed antimicrobial activity were later tested to determine the Minimum Inhibitory Dilution (MID) for those bacteria. They were also effective on the inhibition of DNase activity. This study was indicated that extracts and essential oils of Black and Red pepper and Thyme can play a significant role in inhibition of Escherichia coli O157: H7 and Staphylococcus aureus. PMID:24250643

  10. Essential oils from fruits with different colors and leaves of Neomitranthes obscura (DC.) N. Silveira: an endemic species from Brazilian Atlantic forest.

    PubMed

    Amaral, Raquel R; Fernandes, Caio P; Caramel, Otávio P; Tietbohl, Luis A C; Santos, Marcelo G; Carvalho, José C T; Rocha, Leandro

    2013-01-01

    Neomitranthes obscura (DC.) N. Silveira is an endemic plant of Brazilian Atlantic Forest and widely spread in the sandbanks of "Restinga de Jurubatiba" National Park. It is popularly known by local population as "camboim-de-cachorro" or "cambuí-preto" and recognized by its black ripe fruits. However, specimens with yellow ripe fruits were localized in the "Restinga de Jurubatiba" National Park. The aim of the present study was to evaluate chemical composition of essential oils obtained from leaves and fruits of N. obscura specimens with different fruit color (black and yellow) by GC and GC-MS. Essential oils from leaves of specimens with black and yellow fruits indicated a predominance of sesquiterpenes (81.1% and 84.8%, resp.). Meanwhile, essential oil from black fruits presented a predominance of monoterpenes (50.5%), while essential oil from yellow fruits had sesquiterpenes (39.9%) as major substances. Despite previous studies about this species, including essential oil extraction, to our knowledge this is the first report on N. obscura fruits with different colors. Our results suggest the occurrence of unless two different varieties for this species.

  11. Essential Oils from Fruits with Different Colors and Leaves of Neomitranthes obscura (DC.) N. Silveira: An Endemic Species from Brazilian Atlantic Forest

    PubMed Central

    Amaral, Raquel R.; Fernandes, Caio P.; Caramel, Otávio P.; Tietbohl, Luis A. C.; Santos, Marcelo G.; Carvalho, José C. T.; Rocha, Leandro

    2013-01-01

    Neomitranthes obscura (DC.) N. Silveira is an endemic plant of Brazilian Atlantic Forest and widely spread in the sandbanks of “Restinga de Jurubatiba” National Park. It is popularly known by local population as “camboim-de-cachorro” or “cambuí-preto” and recognized by its black ripe fruits. However, specimens with yellow ripe fruits were localized in the “Restinga de Jurubatiba” National Park. The aim of the present study was to evaluate chemical composition of essential oils obtained from leaves and fruits of N. obscura specimens with different fruit color (black and yellow) by GC and GC-MS. Essential oils from leaves of specimens with black and yellow fruits indicated a predominance of sesquiterpenes (81.1% and 84.8%, resp.). Meanwhile, essential oil from black fruits presented a predominance of monoterpenes (50.5%), while essential oil from yellow fruits had sesquiterpenes (39.9%) as major substances. Despite previous studies about this species, including essential oil extraction, to our knowledge this is the first report on N. obscura fruits with different colors. Our results suggest the occurrence of unless two different varieties for this species. PMID:23484148

  12. Analysis of heavy metal lead (Pb) levels with Aas in cow's milk by giving cumin (Cuminum cyminum L.), white turmeric (Curcuma zedoaria Rosc.) and mango turmeric (Curcuma mangga Val.).

    PubMed

    Nurdin, E; Putra, D P; Amelia, T

    2013-11-01

    The aim of this study was to determine the effect of giving Cumin (Cuminum cyminum L.), White Turmeric (Curcuma zedoaria Rosc.) and Mango Turmeric (Curcuma mango Val.) on levels of heavy metals lead (Pb) in cow's milk produced. The study was conducted in West Java with experimental method in 16 Fries Holland dairy cows with lactation period of 2-4 months and lactation months of 3-4 months. The design used is simple randomized design with 4 treatments such as Group A (control/no treatment), Group B (Cumin 0.03% body weight), Group C (White Turmeric 0.02% body weight) and Group D (Mango Turmeric 0.06% body weight). Measurement of Pb levels in milk using the method of wet destruction, while Pb measurements on faeces using wet ashing method, by means of Atomic Absorption Spectrophotometry. Based on the researsch results showed that administration of Cumin, White Turmeric and Mango Turmeric have very real effect on reducing levels of heavy metals lead (Pb) in cow's milk produced, with a consecutive decrease 98.36, 99.33 and 99.37% and the very real effect on elevated levels of Pb in faeces by 68.01, 64.52 and 80.54%. Mango Turmeric is the best treatment of three treatment in decreasing lead level in milk.

  13. Cancer Cell Signaling Pathways Targeted by Spice-Derived Nutraceuticals

    PubMed Central

    Sung, Bokyung; Prasad, Sahdeo; Yadav, Vivek R.; Aggarwal, Bharat B.

    2012-01-01

    Extensive research within the last half a century has revealed that cancer is caused by dysregulation of as many as 500 different gene products. Most natural products target multiple gene products and thus are ideally suited for prevention and treatment of various chronic diseases, including cancer. Dietary agents such as spices have been used extensively in the Eastern world for a variety of ailments for millennia, and five centuries ago they took a golden journey to the Western world. Various spice-derived nutraceuticals, including 1′-acetoxychavicol acetate, anethole, capsaicin, car-damonin, curcumin, dibenzoylmethane, diosgenin, eugenol, gambogic acid, gingerol, thymoquinone, ursolic acid, xanthohumol, and zerumbone derived from galangal, anise, red chili, black cardamom, turmeric, licorice, fenugreek, clove, kokum, ginger, black cumin, rosemary, hop, and pinecone ginger, respectively, are the focus of this review. The modulation of various transcription factors, growth factors, protein kinases, and inflammatory mediators by these spice-derived nutraceuticals are described. The anticancer potential through the modulation of various targets is also the subject of this review. Although they have always been used to improve taste and color and as a preservative, they are now also used for prevention and treatment of a wide variety of chronic inflammatory diseases, including cancer. PMID:22149093

  14. Could essential oils of green and black pepper be used as food preservatives?

    PubMed

    Nikolić, Miloš; Stojković, Dejan; Glamočlija, Jasmina; Ćirić, Ana; Marković, Tatjana; Smiljković, Marija; Soković, Marina

    2015-10-01

    Black and green pepper essential oils were used in this study in order to determine the chemical composition, in vitro antimicrobial activity against food spoilage microorganisms and in situ oils effect on food microorganism, after incorporation in chicken soup, by suggested methodology for calculation of Growth inhibition concentrations (GIC50). Chemical analysis revealed a total of 34 components. The major constituent of black pepper oil was trans-caryophyllene (30.33 %), followed by limonene (12.12 %), while β-pinene (24.42 %), δ(3)-carene (19.72 %), limonene (18.73 %) and α-pinene (10.39 %) were dominant compounds in green pepper oil. Antimicrobial activity was determined by microdilution technique and minimal inhibitory (MIC) and minimal bactericidal/fungicidal concentrations (MBC/MFC) were determined. Green pepper oil showed stronger antibacterial and antifungal activity (MIC 0.50-1.87; MBC 0.63-2.5 mg/ml; MIC 0.07-0.16; MFC 0.13-1.25 mg/ml) against black pepper oil (MIC 0.07-3.75; MBC 0.60-10.00 mg/ml; MIC 0.63-5.00; MFC 1.25-10.00 mg/ml. Oils successfully inhibited the growth of S. aureus in chicken soup in a dose dependent manner. GIC50 values were calculated after 24, 48 and 72 h and were in range of 0.156-0.689 mg/ml. The 50 % inhibitory concentrations (IC50) of EOs were 36.84 and 38.77 mg/ml with in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay respectively. The obtained results revealed that black and green pepper volatiles are efficient in controlling the growth of known food-spoilage microorganisms.

  15. Antimicrobial activity of the bioactive components of essential oils from Pakistani spices against Salmonella and other multi-drug resistant bacteria

    PubMed Central

    2013-01-01

    Background The main objective of this study was the phytochemical characterization of four indigenous essential oils obtained from spices and their antibacterial activities against the multidrug resistant clinical and soil isolates prevalent in Pakistan, and ATCC reference strains. Methods Chemical composition of essential oils from four Pakistani spices cumin (Cuminum cyminum), cinnamon (Cinnamomum verum), cardamom (Amomum subulatum) and clove (Syzygium aromaticum) were analyzed on GC/MS. Their antibacterial activities were investigated by minimum inhibitory concentration (MIC) and Thin-Layer Chromatography-Bioautographic (TLC-Bioautographic) assays against pathogenic strains Salmonella typhi (D1 Vi-positive), Salmonella typhi (G7 Vi-negative), Salmonella paratyphi A, Escherichia coli (SS1), Staphylococcus aureus, Pseudomonas fluorescens and Bacillus licheniformis (ATCC 14580). The data were statistically analyzed by using Analysis of Variance (ANOVA) and Least Significant Difference (LSD) method to find out significant relationship of essential oils biological activities at p <0.05. Results Among all the tested essential oils, oil from the bark of C. verum showed best antibacterial activities against all selected bacterial strains in the MIC assay, especially with 2.9 mg/ml concentration against S. typhi G7 Vi-negative and P. fluorescens strains. TLC-bioautography confirmed the presence of biologically active anti-microbial components in all tested essential oils. P. fluorescens was found susceptible to C. verum essential oil while E. coli SS1 and S. aureus were resistant to C. verum and A. subulatum essential oils, respectively, as determined in bioautography assay. The GC/MS analysis revealed that essential oils of C. cyminum, C. verum, A. subulatum, and S. aromaticum contain 17.2% cuminaldehyde, 4.3% t-cinnamaldehyde, 5.2% eucalyptol and 0.73% eugenol, respectively. Conclusions Most of the essential oils included in this study possessed good antibacterial activities against selected multi drug resistant clinical and soil bacterial strains. Cinnamaldehyde was identified as the most active antimicrobial component present in the cinnamon essential oil which acted as a strong inhibitory agent in MIC assay against the tested bacteria. The results indicate that essential oils from Pakistani spices can be pursued against multidrug resistant bacteria. PMID:24119438

  16. Effect of carbon black composition with sludge palm oil on the curing characteristic and mechanical properties of natural rubber/styrene butadiene rubber compound

    NASA Astrophysics Data System (ADS)

    Mohamed, R.; Nurazzi, N. Mohd; Huzaifah, M.

    2017-07-01

    This study was conducted to investigate the possibility of utilizing sludge palm oil (SPO) as processing oil, with various amount of carbon black as its reinforcing filler, and its effects on the curing characteristics and mechanical properties of natural rubber/styrene butadiene rubber (NR/SBR) compound. Rubber compound with fixed 15 pphr of SPO loading, and different carbon black loading from 20 to 50 pphr, was prepared using two roll mills. The cure characteristics and mechanical tests that have been conducted are the scorch and cure time analysis, tensile strength and tear strength. Scorch time (ts5) and cure time (t90) of the compound increases with the increasing carbon black loading. The mechanical properties of NR/SBR compound viz. the tensile strength, modulus at 300% strain and tear strength were also improved by the increasing carbon black loading.

  17. Listeriosis Phytotherapy

    PubMed Central

    Rafieian-Kopaei, Mahmoud; Saki, Kourosh; Bahmani, Mahmoud; Ghafourian, Sobhan; Sadeghifard, Nourkhoda; Taherikalani, Morovat

    2015-01-01

    Listeria monocytogenes can be found in many processed foods, raw milk, dairy products, meat and meat products such as sausages, beef and fish products, seafoods, eggs, fruits, and vegetables such as radish and cabbage. This article is a review study on the Iranian medicinal plants applied for treatment of listeriosis. Information of this review article was obtained by searching various key words such as Listeria monocytogenes, medicinal plants, plant extracts and essential oils among scientific articles published in databases of Google scholar, ISI Web of Knowledge, PubMed, Scopus, SID and Magiran. Thyme, German chamomile, great chamomile, yarrow, onion, oregano, nutmeg, sage, sagebrush, hyssop, rosemary, St John’s wort, safflower, ajowan, cumin, peppermint, shallot, anise, and parsnip are known antilisteriosis medicinal plants. Bioactive phytochemicals, antioxidants and monoterpenes, sesquiterpene, coumarin, flavonoids, tannins, saponins, alkaloids, and terpenoids are the main ingredients of antilisteriosis medicinal plants. PMID:26682768

  18. Dietary Natural Products for Prevention and Treatment of Breast Cancer.

    PubMed

    Li, Ya; Li, Sha; Meng, Xiao; Gan, Ren-You; Zhang, Jiao-Jiao; Li, Hua-Bin

    2017-07-08

    Breast cancer is the most common cancer among females worldwide. Several epidemiological studies suggested the inverse correlation between the intake of vegetables and fruits and the incidence of breast cancer. Substantial experimental studies indicated that many dietary natural products could affect the development and progression of breast cancer, such as soy, pomegranate, mangosteen, citrus fruits, apple, grape, mango, cruciferous vegetables, ginger, garlic, black cumin, edible macro-fungi, and cereals. Their anti-breast cancer effects involve various mechanisms of action, such as downregulating ER-α expression and activity, inhibiting proliferation, migration, metastasis and angiogenesis of breast tumor cells, inducing apoptosis and cell cycle arrest, and sensitizing breast tumor cells to radiotherapy and chemotherapy. This review summarizes the potential role of dietary natural products and their major bioactive components in prevention and treatment of breast cancer, and special attention was paid to the mechanisms of action.

  19. Dietary Natural Products for Prevention and Treatment of Breast Cancer

    PubMed Central

    Li, Ya; Li, Sha; Meng, Xiao; Zhang, Jiao-Jiao

    2017-01-01

    Breast cancer is the most common cancer among females worldwide. Several epidemiological studies suggested the inverse correlation between the intake of vegetables and fruits and the incidence of breast cancer. Substantial experimental studies indicated that many dietary natural products could affect the development and progression of breast cancer, such as soy, pomegranate, mangosteen, citrus fruits, apple, grape, mango, cruciferous vegetables, ginger, garlic, black cumin, edible macro-fungi, and cereals. Their anti-breast cancer effects involve various mechanisms of action, such as downregulating ER-α expression and activity, inhibiting proliferation, migration, metastasis and angiogenesis of breast tumor cells, inducing apoptosis and cell cycle arrest, and sensitizing breast tumor cells to radiotherapy and chemotherapy. This review summarizes the potential role of dietary natural products and their major bioactive components in prevention and treatment of breast cancer, and special attention was paid to the mechanisms of action. PMID:28698459

  20. Detecting gas hydrate behavior in crude oil using NMR.

    PubMed

    Gao, Shuqiang; House, Waylon; Chapman, Walter G

    2006-04-06

    Because of the associated experimental difficulties, natural gas hydrate behavior in black oil is poorly understood despite its grave importance in deep-water flow assurance. Since the hydrate cannot be visually observed in black oil, traditional methods often rely on gas pressure changes to monitor hydrate formation and dissociation. Because gases have to diffuse through the liquid phase for hydrate behavior to create pressure responses, the complication of gas mass transfer is involved and hydrate behavior is only indirectly observed. This pressure monitoring technique encounters difficulties when the oil phase is too viscous, the amount of water is too small, or the gas phase is absent. In this work we employ proton nuclear magnetic resonance (NMR) spectroscopy to observe directly the liquid-to-solid conversion of the water component in black oil emulsions. The technique relies on two facts. The first, well-known, is that water becomes essentially invisible to liquid state NMR as it becomes immobile, as in hydrate or ice formation. The second, our recent finding, is that in high magnetic fields of sufficient homogeneity, it is possible to distinguish water from black oil spectrally by their chemical shifts. By following changes in the area of the water peak, the process of hydrate conversion can be measured, and, at lower temperatures, the formation of ice. Taking only seconds to accomplish, this measurement is nearly direct in contrast to conventional techniques that measure the pressure changes of the whole system and assume these changes represent formation or dissociation of hydrates - rather than simply changes in solubility. This new technique clearly can provide accurate hydrate thermodynamic data in black oils. Because the technique measures the total mobile water with rapidity, extensions should prove valuable in studying the dynamics of phase transitions in emulsions.

  1. Cuminum cyminum extract attenuates scopolamine-induced memory loss and stress-induced urinary biochemical changes in rats: a noninvasive biochemical approach.

    PubMed

    Koppula, Sushruta; Choi, Dong Kug

    2011-07-01

    Cuminum cyminum Linn. (Apiaceae), cumin, is a popular spice with a long history of medicinal use to treat various symptoms such as diarrhea, flatulence, gynecological, and respiratory diseases. To date, no scientific investigation was reported regarding memory-enhancing and antistress activity of cumin fruits. The present study deals with the memory-enhancing and antistress activities and further the antioxidant status via lipid peroxidation inhibition. Antistress activity was evaluated by inducing stress via forced swimming and the urinary vanillylmandelic acid (VMA) and ascorbic acid were estimated as biomarkers. Memory-enhancing activity was studied by conditioned avoidance response using Cook's pole climbing apparatus in normal and scopolamine-induced amnestic rats. Thiobarbituric acid reactive substances (TBARS) assay was used to evaluate the lipid peroxidation. Daily administration of cumin at doses of 100, 200, and 300 mg/kg body weight 1 h prior to induction of stress inhibited the stress-induced urinary biochemical changes in a dose-dependent manner without altering the levels in normal control groups. The cognition, as determined by the acquisition, retention, and recovery in rats, was observed to be dose-dependent. The extract also produced significant lipid peroxidation inhibition in comparison with known antioxidant ascorbic acid in both rat liver and brain. This study provides scientific support for the antistress, antioxidant, and memory-enhancing activities of cumin extract and substantiates that its traditional use as a culinary spice in foods is beneficial and scientific in combating stress and related disorders.

  2. The Black Hull Fleet: Multi-Function Assets for Multi-Mission Duty

    DTIC Science & Technology

    2011-01-01

    also conduct annual training and mock ex- ercises using pre-staged vessel of opportunity skimming system gear, and all crews receive hazardous waste...Valdez oil spill in 1989, the Oil Pollution Act of 1990 mandated that they be outfittedwith an onboard spilled oil recovery system (SORS) comprised of... outriggers , booms, hydraulic skim- ming equipment, and product storage vessels. The“Black Hull” Fleet Multi-function assets for multi-mission duty. by

  3. More wells will expand knowledge of Knox group, Black Warrior basin

    USGS Publications Warehouse

    Raymond, Dorothy E.

    1991-01-01

    The Cambrian-Ordovician Knox group of the Black Warrior basin in Alabama and Mississippi has attracted the interest of the oil industry because of recent significant discoveries of oil and gas in the age-equivalent Arbuckle group carbonates of the Arkoma, Ardmore, and Anadarko basins of Oklahoma. The geologic setting of these areas is described. Oil and gas potential is assessed and the Knox production history is given. Source rock potential is outlined.

  4. A Comprehensive Review on Rasam: A South Indian Traditional Functional Food.

    PubMed

    Devarajan, Agilandeswari; Mohanmarugaraja, M K

    2017-01-01

    The view that food can have an expanded role that goes well beyond providing a source of nutrients truly applies to traditional functional foods. The systematic consumption of such traditional functional food provides an excellent preventive measure to ward off many diseases. Rasam , a soup of spices, is a traditional South Indian food. It is traditionally prepared using tamarind juice as a base, with the addition of Indian sesame oil, turmeric, tomato, chili pepper, pepper, garlic, cumin, curry leaves, mustard, coriander, asafoetida, sea salt, and water. Rasam is a classic example of traditional functional food with all its ingredients medicinally claimed for various ailments. The preclinical and clinical studies on rasam and its ingredients support their traditional claim. This review is an attempt to compile the literatures on rasam , its ingredients, and to highlight its medicinal potential that has been underestimated.

  5. A Comprehensive Review on Rasam: A South Indian Traditional Functional Food

    PubMed Central

    Devarajan, Agilandeswari; Mohanmarugaraja, M. K.

    2017-01-01

    The view that food can have an expanded role that goes well beyond providing a source of nutrients truly applies to traditional functional foods. The systematic consumption of such traditional functional food provides an excellent preventive measure to ward off many diseases. Rasam, a soup of spices, is a traditional South Indian food. It is traditionally prepared using tamarind juice as a base, with the addition of Indian sesame oil, turmeric, tomato, chili pepper, pepper, garlic, cumin, curry leaves, mustard, coriander, asafoetida, sea salt, and water. Rasam is a classic example of traditional functional food with all its ingredients medicinally claimed for various ailments. The preclinical and clinical studies on rasam and its ingredients support their traditional claim. This review is an attempt to compile the literatures on rasam, its ingredients, and to highlight its medicinal potential that has been underestimated. PMID:28989243

  6. Listeriosis Phytotherapy: A Review Study on the Effectiveness of Iranian Medicinal Plants in Treatment of Listeriosis.

    PubMed

    Rafieian-Kopaei, Mahmoud; Saki, Kourosh; Bahmani, Mahmoud; Ghafourian, Sobhan; Sadeghifard, Nourkhoda; Taherikalani, Morovat

    2015-12-17

    Listeria monocytogenes can be found in many processed foods, raw milk, dairy products, meat and meat products such as sausages, beef and fish products, seafoods, eggs, fruits, and vegetables such as radish and cabbage. This article is a review study on the Iranian medicinal plants applied for treatment of listeriosis. Information of this review article was obtained by searching various key words such as Listeria monocytogenes, medicinal plants, plant extracts and essential oils among scientific articles published in databases of Google scholar, ISI Web of Knowledge, PubMed, Scopus, SID and Magiran. Thyme, German chamomile, great chamomile, yarrow, onion, oregano, nutmeg, sage, sagebrush, hyssop, rosemary, St John's wort, safflower, ajowan, cumin, peppermint, shallot, anise, and parsnip are known antilisteriosis medicinal plants. Bioactive phytochemicals, antioxidants and monoterpenes, sesquiterpene, coumarin, flavonoids, tannins, saponins, alkaloids, and terpenoids are the main ingredients of antilisteriosis medicinal plants. © The Author(s) 2015.

  7. Chemical composition and antimicrobial activity of the essential oil of endemic Dalmatian black pine (Pinus nigra ssp. dalmatica).

    PubMed

    Politeo, Olivera; Skocibusic, Mirjana; Maravic, Ana; Ruscic, Mirko; Milos, Mladen

    2011-03-01

    The chemical composition and the antimicrobial activity of the essential oil isolated from the needles of endemic Dalmatian black pine (Pinus nigra ssp. dalmatica) from Croatia were investigated. The chemical composition of the essential oil was determined by GC and GC/MS analyses, and the main compounds identified were α-pinene, β-pinene, germacrene D, and β-caryophyllene. Disc-diffusion and broth-microdilution assays were used for the in vitro antimicrobial screening. The Dalmatian black pine essential oil exhibited a great potential of antibacterial activity against Gram-positive bacteria (MIC=0.03-0.50% (v/v)) and a less pronounced activity against Gram-negative bacteria (MIC=0.12-3.2% (v/v)). The volatile compounds also inhibited the growth of all fungi tested, including yeast. Copyright © 2011 Verlag Helvetica Chimica Acta AG, Zürich.

  8. Geochemical changes in crude oil spilled from the Exxon Valdez supertanker into Prince William Sound, Alaska

    USGS Publications Warehouse

    Hostettler, Frances D.; Kvenvolden, Keith A.

    1994-01-01

    North Slope crude oil spilled from the T/V Exxon Valdez in March 1989 and contaminated about 500 km of Prince William Sound shoreline. Aliphatic and aromatic hydrocarbons in oil samples collected in August 1990 and June 1992 from beaches on six islands impacted by the spill have been compared with the hydrocarbons from North Slope crude oil taken from the stricken tanker. Degradation processes have changed the physical appearance of this residual spilled oil; the beached oil as collected ranged from a light brown color, to a heavy black viscous oil, to a black, powder-like residue. In these physically different samples, terpane, sterane, and aromatic sterane distributions, as well as carbon isotope values, are similar and correlate with the original Exxon Valdez oil. On the other hand, n-alkanes, isoprenoids, and many of the polycyclic aromatic hydrocarbons which are present in the original crude oil are dramatically altered in the oil samples collected from the beaches.

  9. Aspergillus parasiticus growth and aflatoxin production on black and white pepper and the inhibitory action of their chemical constituents.

    PubMed Central

    Madhyastha, M S; Bhat, R V

    1984-01-01

    Aspergillus parasiticus Speare NRRL 2999 growth and aflatoxin production in black and white pepper and the penetration of the fungus in black pepper corn over various incubation periods were studied. Also, the effects of piperine and pepper oil on growth and aflatoxin production were studied. Under laboratory conditions, black and white pepper supported aflatoxin production (62.5 and 44 ppb (ng/g), respectively) over 30 days of incubation. Fungal growth measured in terms of chitin was considerably less in white pepper than in black pepper. A histological study of black pepper corn showed the fungus penetrating up to the inner mesocarp and establishing itself in the middle mesocarp. Piperine and pepper oil were found to inhibit fungal growth and toxin production in a dose-dependent manner. Thus, both black and white pepper could be considered as poor substrates for fungal growth and aflatoxin production. Images PMID:6435523

  10. Black pepper essential oil to enhance intravenous catheter insertion in patients with poor vein visibility: a controlled study.

    PubMed

    Kristiniak, Susan; Harpel, Jean; Breckenridge, Diane M; Buckle, Jane

    2012-11-01

    To evaluate the effect of topically applied black pepper essential oil on easing intravenous catheter insertion (IVC) in patients with no palpable or visible veins compared to a control group (standard nursing practice). Randomized, controlled study. One hundred twenty hospitalized patients, who were referred to a hospital vascular team because of difficulty in accessing veins for IVC insertion. Topical application of 20% essential oil of black pepper in aloe vera gel or standard nursing care (hot packs with or without vigorous tactile stimulation). Pre- and post-test vein visibility and/or palpability and number of attempts at IVC insertion. A higher percentage of patients achieved optimal scoring (vein score=2) or improved scoring (vein score of 1 or 2) to black pepper intervention than standard nursing care. The black pepper group also reduced the number of patients whose veins were still not visible or palpable after the intervention to nearly half that of the control group (p<0.05). The number of IVC attempts following black pepper was also half that of the control group. Topical application of black pepper is a viable and effective way to enhance vein visibility and palpability prior to intravenous insertion in patients with limited vein accessibility; it also improves ease of IVC insertion.

  11. Nutraceutical properties of cumin residue generated from Ayurvedic industries using cell line models.

    PubMed

    Arun, K B; Aswathi, U; Venugopal, V V; Madhavankutty, T S; Nisha, P

    2016-10-01

    Spent cumin (SC), generated from Ayurvedic industry, was evaluated for its nutraceutical potential in terms of antioxidant, antidiabetic and anticancer properties, and compared with that of the raw cumin (RC). SC and RC seeds were extracted with ethyl acetate (E) and methanol (M). SCM (methanol extract) were rich in p-coumaric acid, ferulic acid, ellagic acid and cinnamic acid (6.4445, 5.8286, 2.1519, 4.3085 mg/g dry extract). SCM reduced Fe 2+ ion (89.68 µM AA/g dry weight), scavenged DPPH radical (IC 50 -238.6 µg/mL), better α-amylase inhibition (IC 50 -337.22 µg/mL) and glucose uptake activity in 30.7% of L6 cells. SCM inhibited viability, retarded migration area up to 41.02%, arrested cell cycle at S phase and induced apoptosis in 2.45% of HT29 colon cancer cells. The results indicated that dietary interventions using nutraceutical food formulation made out of SC can play a significant role in the prevention and management of degenerative diseases.

  12. Modification of deoiled cumin dietary fiber with laccase and cellulase under high hydrostatic pressure.

    PubMed

    Ma, Mengmei; Mu, Taihua

    2016-01-20

    In this study, we evaluated the effects of high hydrostatic pressure (HHP) and enzyme (laccase and cellulase) treatment on the structural, physicochemical, and functional properties and antioxidant activity of deoiled cumin dietary fiber (DF). HHP-enzyme treatment increased the contents of soluble dietary fiber (SDF) (30.37 g/100g), monosaccharides (except for glucose), uronic acids, and total polyphenol. HHP-enzyme treatment altered the honey-comb structure of DF and generated new polysaccharides. DF modified by HHP-enzyme treatment exhibited improved water retention capacity (10.02 g/g), water swelling capacity (11.19 mL/g), fat and glucose absorption capacities (10.44 g/g, 22.18-63.54 mmol/g), α-amylase activity inhibition ration (37.95%), and bile acid retardation index (48.85-52.58%). The antioxidant activity of DF was mainly correlated to total polyphenol content (R=0.8742). Therefore, DF modified by HHP-enzyme treatment from deoiled cumin could be used as a fiber-rich ingredient in functional foods. Copyright © 2015. Published by Elsevier Ltd.

  13. Formation resistivity as an indicator of oil generation in black shales

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hester, T.C.; Schmoker, J.W.

    1987-08-01

    Black, organic-rich shales of Late Devonian-Early Mississippi age are present in many basins of the North American craton and, where mature, have significant economic importance as hydrocarbon source rocks. Examples drawn from the upper and lower shale members of the Bakken Formation, Williston basin, North Dakota, and the Woodford Shale, Anadarko basin, Oklahoma, demonstrate the utility of formation resistivity as a direct in-situ indicator of oil generation in black shales. With the onset of oil generation, nonconductive hydrocarbons begin to replace conductive pore water, and the resistivity of a given black-shale interval increases from low levels associated with thermal immaturitymore » to values approaching infinity. Crossplots of a thermal-maturity index (R/sub 0/ or TTI) versus formation resistivity define two populations representing immature shales and shales that have generated oil. A resistivity of 35 ohm-m marks the boundary between immature and mature source rocks for each of the three shales studied. Thermal maturity-resistivity crossplots make possible a straightforward determination of thermal maturity at the onset of oil generation, and are sufficiently precise to detect subtle differences in source-rock properties. For example, the threshold of oil generation in the upper Bakken shale occurs at R/sub 0/ = 0.43-0.45% (TTI = 10-12). The threshold increases to R/sub 0/ = 0.48-0.51% (TTI = 20-26) in the lower Bakken shale, and to R/sub 0/ = 0.56-0.57% (TTI = 33-48) in the most resistive Woodford interval.« less

  14. Analysis of Insect toxicity and repellent activity of Phytochemicals from "Skimmia laureola, Nair" against "Black garden ant, Lasius niger" of Pakistan.

    PubMed

    Mehmood, Ferhat; Khan, Zaheer-ud-Din; Manzoor, Farkhanda; Jamil, Muhammad

    2016-05-01

    A study was conducted to evaluate the toxicity and repellency of essential oils from root, stem and leaves of Nazar panra, Skimmia laureola (DC.) Zucc. Ex Walp. of family (Sapindales: Rutaceae) ver. Nair of Pakistan. The oils were tested at three concentrations i.e. 1, 5 and 10%. Black garden ant, Lasius niger L. (Hymenoptera: Formicidae) of Pakistan were selected and exposed to essential oils at room temperature. All essential oils showed Insecticidal activity with LC(50)=10.15, while dose dependant effect was significant with R(2)=0.98. It can be concluded that the three Essential oils in this study have both Insecticidal as well as repellent effect.

  15. Oil-source correlations between the Mississippian Heath Shales and the reservoired oils in the Pennsylvanian Tyler Sands, Montana

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Cole, G.A.; Drozd, R.J.; Daniel, J.A.

    The Mississippi Heath Formation exposed in Fergus County, central Montana, is comprised predominantly of nearshore, marine, black, calcareous shales and carbonates with minor anhydrite and coal beds. The black shales and limestones have been considered as sources for shale oil via Fischer Assay and pyrolysis analysis. These shales are potential source units for the oils reservoired in the overlying Pennsylvanian Tyler Formation sands located 50 mi (80 km) to the east of the Fergus County Heath sediment studied. Heath Formation rocks from core holes were selectively sampled in 2-ft increments and analyzed for their source rock characteristics. Analyses include percentmore » total organic carbon (%TOC), Rock-Eval pyrolysis, pyrolysis-gas chromatography, and characterization of the total soluble extracts using carbon isotopes and gas chromatography-mass Spectrometry. Results indicated that the Heath was an excellent potential source unit that contained oil-prone, organic-rich (maximum of 17.6% TOC), calcareous, black shale intervals. The Heath and Tyler formations also contained intervals dominated by gas-prone, organic-rich shales of terrestrial origin. Three oils from the Tyler Formation sands in Musselshell and Rosebud counties were characterized by similar methods as the extracts. The oils were normally mature, moderate API gravity, moderate sulfur, low asphaltene crudes. Oil to source correlations between the Heath shale extracts and the oils indicated the Heath was an excellent candidate source rock for the Tyler reservoired oils. Conclusions were based on excellent matches between the carbon isotopes of the oils and the kerogen-kerogen pyrolyzates, and from the biomarkers.« less

  16. Comparison of oilseed yields: a preliminary review

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Duke, J.A.; Bagby, M.O.

    It was assumed that for most oilseed crops, 90% of the oil yield might be considered as profit. To compare oil seeds, pertinent portions of the yield and energy paragraphs from a summary published by Dr. Duke for DOE Grant No. 59-2246-1-6-054-0 with Dr. Bagby as ADODR were reproduced. The seed yields ranged from 200 to 14,000 kg/ha, the low one too low to consider and the high one suspiciously high. The yield of 14,000 kg oil per hectare is equivalent to more than 30 barrels of oil per hectare. The energy species included ambrette, tung-oil tree, cashew, wood-oil tree,more » mu-oil tree, peanut, mustard greens; rape, colza; black mustard, turnip, safflower, colocynth, coconut, crambe, African oil palm, soybean, cotton, sunflower, Eastern black walnut, Engligh walnut, meadow foam, flax, macadamia nuts, opium poppy, perilla, almond, castorbean, Chinese tallow tree, sesame, jojoba, yellow mustard, stokes' aster, and Zanzibar oilvine. 1 table. (DP)« less

  17. Optimization of extraction efficiency by shear emulsifying assisted enzymatic hydrolysis and functional properties of dietary fiber from deoiled cumin (Cuminum cyminum L.).

    PubMed

    Ma, Mengmei; Mu, Taihua; Sun, Hongnan; Zhang, Miao; Chen, Jingwang; Yan, Zhibin

    2015-07-15

    This study evaluated the optimal conditions for extracting dietary fiber (DF) from deoiled cumin by shear emulsifying assisted enzymatic hydrolysis (SEAEH) using the response surface methodology. Fat adsorption capacity (FAC), glucose adsorption capacity (GAC), and bile acid retardation index (BRI) were measured to evaluate the functional properties of the extracted DF. The results revealed that the optimal extraction conditions included an enzyme to substrate ratio of 4.5%, a reaction temperature of 57 °C, a pH value of 7.7, and a reaction time of 155 min. Under these conditions, DF extraction efficiency and total dietary fiber content were 95.12% and 84.18%, respectively. The major components of deoiled cumin DF were hemicellulose (37.25%) and cellulose (33.40%). FAC and GAC increased with decreasing DF particle size (51-100 μm), but decreased with DF particle sizes <26 μm; BRI increased with decreasing DF particle size. The results revealed that SEAEH is an effective method for extracting DF. DF with particle size 26-51 μm had improved functional properties. Copyright © 2015. Published by Elsevier Ltd.

  18. Development of a workflow for screening and identification of α-amylase inhibitory peptides from food source using an integrated Bioinformatics-phage display approach: Case study - Cumin seed.

    PubMed

    Siow, Hwee-Leng; Lim, Theam Soon; Gan, Chee-Yuen

    2017-01-01

    The main objective of this study was to develop an efficient workflow to discover α-amylase inhibitory peptides from cumin seed. A total of 56 unknown peptides was initially found in the cumin seed protein hydrolysate. They were subjected to 2 different in silico screenings and 6 peptides were shortlisted. The peptides were then subjected to in vitro selection using phage display technique and 3 clones (CSP3, CSP4 and CSP6) showed high affinity in binding α-amylase. These clones were subjected to the inhibitory test and only CSP4 and CSP6 exhibited high inhibitory activity. Therefore, these peptides were chemically synthesized for validation purposes. CSP4 exhibited inhibition of bacterial and human salivary α-amylases with IC50 values of 0.11 and 0.04μmol, respectively, whereas CSP6 was about 0.10 and 0.15μmol, respectively. Results showed that the strength of each protocol has been successfully combined as deemed fit to enhance the α-amylase inhibitor peptide discovery. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Bioefficacy of some plant derivatives that protect grain against the pulse beetle, Callosobruchus maculatus

    PubMed Central

    Rahman, A.; Talukder, F. A.

    2006-01-01

    Experiments were conducted to study the bioefficacies of different plant/weed derivatives that affect the development of the pulse beetle, Callosobruchus maculates F. (Coleoptera: Bruchidae) fed on black gram, Vigna mungo, seeds. Plant extracts, powder, ash and oil from nishinda (Vitex negundo L.), eucalyptus (Eucalyptus globules Labill.), bankalmi (Ipomoea sepiaria K.), neem (Azadirachta indica L.), safflower (Carthamus tinctorius L.), sesame (Sesamum indicum L.) and bablah (Acacia arabica L.) were evaluated for their oviposition inhibition, surface protectant, residual toxicity and direct toxicity effects on C. maculates. The results showed that plant oils were effective in checking insect infestation. The least number of F1 adults emerged from black gram seeds treated with neem oil. The nishinda oil extract was the most toxic of three extracts tested (nishinda, eucalyptus and bankalmi). Bablah ash was the most effective compared to the powdered leaves of nishinda, eucalyptus and bankalmi. The powdered leaves and extracts of nishinda, eucalyptus and bankalmi, at a 3% mixture, provided good protection for black gram seeds by reducing insect oviposition, F1 adult emergence, and grain infestation rates. The oil treatment did not show adverse effects on germination capability of seeds, even after three months of treatment. PMID:19537990

  20. Bioefficacy of some plant derivatives that protect grain against the pulse beetle, Callosobruchus maculatus.

    PubMed

    Rahman, A; Talukder, F A

    2006-01-01

    Experiments were conducted to study the bioefficacies of different plant/weed derivatives that affect the development of the pulse beetle, Callosobruchus maculates F. (Coleoptera: Bruchidae) fed on black gram, Vigna mungo, seeds. Plant extracts, powder, ash and oil from nishinda (Vitex negundo L.), eucalyptus (Eucalyptus globules Labill.), bankalmi (Ipomoea sepiaria K.), neem (Azadirachta indica L.), safflower (Carthamus tinctorius L.), sesame (Sesamum indicum L.) and bablah (Acacia arabica L.) were evaluated for their oviposition inhibition, surface protectant, residual toxicity and direct toxicity effects on C. maculates. The results showed that plant oils were effective in checking insect infestation. The least number of F(1) adults emerged from black gram seeds treated with neem oil. The nishinda oil extract was the most toxic of three extracts tested (nishinda, eucalyptus and bankalmi). Bablah ash was the most effective compared to the powdered leaves of nishinda, eucalyptus and bankalmi. The powdered leaves and extracts of nishinda, eucalyptus and bankalmi, at a 3% mixture, provided good protection for black gram seeds by reducing insect oviposition, F(1) adult emergence, and grain infestation rates. The oil treatment did not show adverse effects on germination capability of seeds, even after three months of treatment.

  1. A comparative study on the effect of "black cohosh" and "evening primrose oil" on menopausal hot flashes.

    PubMed

    Mehrpooya, Maryam; Rabiee, Soghra; Larki-Harchegani, Amir; Fallahian, Amir-Mohammad; Moradi, Abbas; Ataei, Sara; Javad, Masoumeh Taravati

    2018-01-01

    Hot flashes are considered to be a common experience for menopausal women and they can compromise the quality of life. The objective of this study is to assess the efficacy of Cimicifuga racemosa in comparison with evening primrose oil (EPO) in postmenopausal women with menopause-related symptoms. This study was performed on 80 postmenopausal women with hot flashes. The participants were randomly divided into two groups by blocked randomization. The participants of one group received black cohosh and the other group received EPO for 8 weeks. The severity and number of hot flashes and quality of life were measured by four-point scale, and the Menopause-Specific Quality of Life (MENQOL) questionnaire at pre-intervention, 1 st , 4 th , and 8 th weeks after treatment. Data were analyzed in SPSS Version 16 using independent t -test, Chi-square, and Fisher's exact test. Average severity of hot flashes in both groups and number of hot flashes in black cohosh group in 8 th week were significantly lower than 1 st week ( P < 0.001), but number of hot flashes in primrose oil group in 8 th week showed no significant differences ( P = 0.32). The number of hot flashes and quality of life score in black cohosh arm compared to EPO showed a significant decrease in the 8 th week ( P < 0.05). All MENQOL scores were significantly improved in two groups ( P < 0.05), but the percentage of improvement in black cohosh arm was significantly superior to EPO group. Both herbs were effective in reduction of severity of hot flashes and improvement of the quality of life, but it seems that black cohosh is more effective than primrose oil because it was able to reduce the number of hot flashes too.

  2. A simple differential pulse polarographic method for the determination of thymoquinone in black seed oil.

    PubMed

    Michelitsch, Astrid; Rittmannsberger, Anna

    2003-01-01

    A reliable and simple differential pulse polarographic method is described for the determination of thymoquinone in black seed oil. The polarographic behaviour of thymoquinone was examined in various buffer systems over the pH range 5.0-10.0. Thymoquinone is reduced in a single, reversible peak at the dropping mercury electrode. The differential pulse polarogram showed a distinct peak in Sörensen buffer:methanol (3:7, v/v; pH 8.5) at a peak potential of -0.095 V (vs. silver/silver chloride electrode), and a plot of peak height against concentration was found to be linear over the range 0.2-15.0 microg/mL (R = 0.9998). The limit of detection was calculated to be 0.054 microg/mL. The polarographic method has been applied to determine thymoquinone in two black seed oil preparations available on the Austrian pharmaceutical market.

  3. Effects of the Exxon Valdez oil spill on migrant shorebirds using rocky intertidal habitats of Prince William Sound, Alaska, during spring, 1989. Exxon Valdez oil spill state/federal natural resource damage assessment final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Martin, P.D.

    1993-12-01

    A minimum of a few 10,000`s of surfbirds (Aphriza virgata) and black turnstones (Arenaria melanocephala) used rocky intertidal habitats of southwestern Prince William sound in spring 1989. Virtually all the shorebirds were found using shorelines, primarily on northern Montague Island, subjectively classified in the field as lightly oiled or unoiled. Surfbirds and black turnstones preyed mainly on herring eggs, blue mussels, and barnacles. Samples of these prey items from oiled areas contained petroleum-derived hydrocarbons, as did at some of the samples from the relatively clean portions of Montague Island. The results of chemical analysis of a small sample of shorebirdmore » liver tissues provided only limited support for the hypothesis that shorebirds had ingested significant quantities of petroleum-derived hydrocarbons. Surfbirds and black turnstones probably escaped significant population impacts as a result of the EVOs because shorelines which received heavy use by these species were largely spared contamination.« less

  4. Aroma compound analysis of Piper nigrum and Piper guineense essential oils from Cameroon using solid-phase microextraction-gas chromatography, solid-phase microextraction-gas chromatography-mass spectrometry and olfactometry.

    PubMed

    Jirovetz, Leopold; Buchbauer, Gerhard; Ngassoum, Martin Benoit; Geissler, Margit

    2002-11-08

    The investigation of aroma compounds of the essential oils of dried fruits of black pepper (Piper nigrum) and black and white "Ashanti pepper" (Piper guineense) from Cameroon by means of solid-phase microextraction (SPME) was carried out for the first time to identify the odorous target components responsible for the characteristic odor of these valuable spices and food flavoring products. By means of GC-flame ionization detection (FID) and GC-MS (using different polar columns) the main compounds (concentration >3.0%, calculated as area of GC-FID analysis using a non-polar fused-silica open tubular RSL-200 column) of the SPME headspace samples of P. nigrum (black) and P. guineense (black and white) were found to be: P. nigrum (black)--germacrene D (11.01%), limonene (10.26%), beta-pinene (10.02%), alpha-phellandrene (8.56%), beta-caryophyllene (7.29%), alpha-pinene (6.40%) and cis-beta-ocimene (3.19%); P. guineense (black)--beta-caryophyllene (57.59%), beta-elemene (5.10%), bicyclogermacrene (5.05%) and alpha-humulene (4.86%); and P. guineense (white)--beta-caryophyllene (51.75%), cis-beta-ocimene (6.61%), limonene (5.88%), beta-pinene (4.56%), linalool (3.97%) and alpha-humulene (3.29%). The most intense odor impressions of the essential oils of the various dried pepper fruits were given byprofessional perfumers as follows: P nigrum (black)--fine, pleasant black pepper note; P. guineense (black)--black pepper top-note; and P. guineense (white)--pleasant white pepper note. These analytical results for the SPME headspace samples of three different pepper species from Cameroon are in accordance with the olfactoric data of the corresponding essential oils. A GC-sniffing technique was used to correlate the single odor impression of the identified SPME headspace volatiles of the three investigated pepper samples with the following results: themain compounds such as beta-caryophyllene, germacrene D, limonene, beta-pinene, alpha-phellandrene and alpha-humulene, as well as minor constituents such as delta-carene, beta-phellandrene, isoborneol, alpha-guaiene, sarisan, elemicin, calamenene, caryophyllene alcohol, isoelemicin, T-muurolol, cubenol and bulnesol, are of greatest importance for the characteristic pepper odor notes of these three Piper samples. Further aroma impressions can be attributed to mono- and sesquiterpenes, hexane, octane and nonane derivatives.

  5. Co-milled silica and coppiced wood biochars improve elongation and toughness in styrene-butadiene elastomeric composites while replacing carbon black

    USDA-ARS?s Scientific Manuscript database

    Carbon black is a petroleum byproduct with a million-ton market in the US tire industry. Finding renewable substitutes for carbon black reduces dependence on oil and alleviates global warming. Biochar is a renewable source of carbon that has been studied previously as a replacement for carbon black ...

  6. Oxygen-Induced Cracking Distillation of Oil in the Continuous Flow Tank Reactor

    ERIC Educational Resources Information Center

    Shvets, Valeriy F.; Kozlovskiy, Roman A.; Luganskiy, Artur I.; Gorbunov, Andrey V.; Suchkov, Yuriy P.; Ushin, Nikolay S.; Cherepanov, Alexandr A.

    2016-01-01

    The article analyses problems of processing black oil fuel and addresses the possibility of increasing the depth of oil refining by a new processing scheme. The study examines various methods of increasing the depth of oil refining reveals their inadequacies and highlights a need to introduce a new method of processing atmospheric and vacuum…

  7. Study on the infrared radiation performance of Tourmaline composite and its effect on the diesel characteristic

    NASA Astrophysics Data System (ADS)

    Liao, Jian Bin; Yu, Hong Liang; Sun, Di; Ma, Fong Yuan

    2017-09-01

    The black tourmaline, magnesium tourmaline, and spinel were ground into powder, and the infrared radiation material was prepared by adding the ceramic powder, clay and the other material into the tourmaline powder according to a certain proportion. The infrared radiation property was tested and analyzed, the diesel was infrared radiation activated by the composite material, and the physicochemical property of fuel oil was analyzed pre-test and post-test. The result shows that the infrared absorption spectrum of the black tourmaline of different particle size is stable. After the diesel oil was infrared radiation activated by tourmaline composite materials, the physicochemical property of diesel oil was changed, the activation energy decreased, the viscosity and surface tension of fuel oil were reduced.

  8. 40 CFR 458.36 - Pretreatment standards for new sources.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... GUIDELINES AND STANDARDS (CONTINUED) CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Channel... pollutant property-Oil and grease. Pretreatment standard-100mg/liter. [60 FR 33972, June 29, 1995] ...

  9. 40 CFR 458.16 - Pretreatment standards for new sources.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... GUIDELINES AND STANDARDS (CONTINUED) CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Furnace... pollutant property-Oil and grease. Pretreatment standard-100mg/liter. [60 FR 33972, June 29, 1995] ...

  10. 40 CFR 458.26 - Pretreatment standards for new sources.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... GUIDELINES AND STANDARDS (CONTINUED) CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Thermal... pollutant property-Oil and grease. Pretreatment standard-100mg/liter. [60 FR 33972, June 29, 1995] ...

  11. 40 CFR 458.36 - Pretreatment standards for new sources.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... GUIDELINES AND STANDARDS (CONTINUED) CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Channel... pollutant property-Oil and grease. Pretreatment standard-100mg/liter. [60 FR 33972, June 29, 1995] ...

  12. 40 CFR 458.26 - Pretreatment standards for new sources.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... GUIDELINES AND STANDARDS (CONTINUED) CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Thermal... pollutant property-Oil and grease. Pretreatment standard-100mg/liter. [60 FR 33972, June 29, 1995] ...

  13. 40 CFR 458.16 - Pretreatment standards for new sources.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... GUIDELINES AND STANDARDS (CONTINUED) CARBON BLACK MANUFACTURING POINT SOURCE CATEGORY Carbon Black Furnace... pollutant property-Oil and grease. Pretreatment standard-100mg/liter. [60 FR 33972, June 29, 1995] ...

  14. Characteristics of PAH tar oil contaminated soils-Black particles, resins and implications for treatment strategies.

    PubMed

    Trellu, Clément; Miltner, Anja; Gallo, Rosita; Huguenot, David; van Hullebusch, Eric D; Esposito, Giovanni; Oturan, Mehmet A; Kästner, Matthias

    2017-04-05

    Tar oil contamination is a major environmental concern due to health impacts of polycyclic aromatic hydrocarbons (PAH) and the difficulty of reaching acceptable remediation end-points. Six tar oil-contaminated soils with different industrial histories were compared to investigate contamination characteristics by black particles. Here we provide a simple method tested on 6 soils to visualize and identify large amounts of black particles (BP) as either solid aggregates of resinified and weathered tar oil or various wood/coke/coal-like materials derived from the contamination history. These materials contain 2-10 times higher PAH concentrations than the average soil and were dominantly found in the sand fraction containing 42-86% of the total PAH. The PAH contamination in the different granulometric fractions was directly proportional to the respective total organic carbon content, since the PAH were associated to the carbonaceous particulate materials. Significantly lower (bio)availability of PAH associated to these carbonaceous phases is widely recognized, thus limiting the efficiency of remediation techniques. We provide a conceptual model of the limited mass transfer of PAH from resinated tar oil phases to the water phase and emphasize the options to physically separate BP based on their lower bulk density and slower settling velocity. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Effect of the cumin cyminum L. Intake on Weight Loss, Metabolic Profiles and Biomarkers of Oxidative Stress in Overweight Subjects: A Randomized Double-Blind Placebo-Controlled Clinical Trial.

    PubMed

    Taghizadeh, Mohsen; Memarzadeh, Mohammad Reza; Asemi, Zatollah; Esmaillzadeh, Ahmad

    2015-01-01

    The current study was performed to determine the effects of cumin cyminum L. intake on weight loss and metabolic profiles among overweight subjects. This randomized double-blind placebo-controlled clinical trial was conducted among 78 overweight subjects (male, n = 18; female, n = 60) aged 18-60 years old. Participants were randomly assigned into three groups to receive: (1) cumin cyminum L. capsule (n = 26); (2) orlistat120 capsule (n = 26) and (3) placebo (n = 26) three times a day for 8 weeks. Anthropometric measures and fasting blood samples were taken at baseline and after 8 weeks of intervention. Consumption of the Cuminum cyminum L. and orlistat120 resulted in a similar significant decrease in weight (-1.1 ± 1.2 and -0.9 ± 1.5 vs. 0.2 ± 1.5 kg, respectively, p = 0.002) and BMI (-0.4 ± 0.5 and -0.4 ± 0.6 vs. 0.1 ± 0.6 kg/m(2), respectively, p = 0.003) compared with placebo. In addition, taking Cuminum cyminum L., compared with orlistat and placebo, led to a significant reduction in serum insulin levels (-1.4 ± 4.5 vs. 1.3 ± 3.3 and 0.3 ± 2.2 µIU/ml, respectively, p = 0.02), HOMA-B (-5.4 ± 18.9 vs. 5.8 ± 13.3 and 1.0 ± 11.0, respectively, p = 0.02) and a significant rise in QUICKI (0.01 ± 0.01 vs. -0.005 ± 0.01 and -0.004 ± 0.01, respectively, p = 0.02). Taking cumin cyminum L. for eight weeks among overweight subjects had the same effects of orlistat120 on weight and BMI and beneficial effects on insulin metabolism compared with orlistat120 and placebo. © 2015 S. Karger AG, Basel.

  16. A High Resolution Study of Black Sea Circulation and Hypothetical Oil Spills

    NASA Astrophysics Data System (ADS)

    Dietrich, D. E.; Bowman, M. J.; Korotenko, K. A.

    2008-12-01

    A 1/24 deg resolution adaptation of the DieCAST ocean model simulates a realistically intense Rim Current and ubiquitous mesoscale coastal anticyclonic eddies that result from anticyclonic vorticity generation by laterally differential bottom drag forces that are amplified near Black Sea coastal headlands. Climatological and synoptic surface forcings are compared. The effects of vertical momentum transfer by known (by Synop region fishermen, as reported by Ballard National Geographic article) big amplitude internal waves are parameterized by big vertical viscosity. Sensitivity to vertical viscosity is shown. Results of simulated hypothetical oil spills are shown. A simple method to nowcast/forecast the Black Sea currents is described and early results are shown.

  17. Reconnaissance for uranium in black shale, Northern Rocky Mountains and Great Plains, 1953

    USGS Publications Warehouse

    Mapel, W.J.

    1954-01-01

    Reconnaissance examinations for uranium in 22 formations containing black shale were conducted in parts of Montana, North Dakota, Utah, Idaho, and Oregon during 1953. About 150 samples from 80 outcrop localities and 5 oil and gas wells were submitted for uranium determinations. Most of the black shale deposits examined contain less than 0.003 percent uranium; however, thin beds of black shale at the base of the Mississippian system contain 0.005 percent uranium at 2 outcrop localities in southwestern Montana and as much as 0.007 percent uranium in a well in northeastern Montana. An eight-foot bed of phosphatic black shale at the base of the Brazer limestone of Late Mississippian age in Rich County, Utah, contains as much as 0.009 percent uranium. Commercial gamma ray logs of oil and gas wells drilled in Montana and adjacent parts of the Dakotas indicate that locally the Heath shale of Late Mississippian age contains as much as 0.01 percent equivalent uranium, and black shales of Late Cretaceous age contain as much as 0.008 percent equivalent uranium.

  18. Blacks in the Army Air Forces during World War II: The Problem of Race Relations

    DTIC Science & Technology

    1977-01-06

    of the Southeast - ern Air Corps Training Center at Maxwell Field, to which Tuskegee was attactied, proceeded with the AAF plan. Black pilots were to...was the most unfavorable. Only in oil capacity did it rank second.5 Daily inconveniences at God- man were that the terrain was unsuitable for night...bombers that struck such objec- Activated on 1 Jul 1947. tives as oil refineries, factories, Equipped with P-47’s. hnacti- airfields, and marshalling

  19. A direct reflection OLVF debris detector based on dark-field imaging

    NASA Astrophysics Data System (ADS)

    Li, Bo; Xi, Yinhu; Feng, Song; Mao, Junhong; Xie, You-Bai

    2018-06-01

    To solve the problems of monitoring wear debris in black oil, a direct reflection online visual ferrograph (OLVF) debris detector is presented. In current OLVF detectors, a reflected light source is used. The emitted light is reflected by wear debris directly instead of passing through the lube oil. Therefore, the transparency of the lube oil ceases to matter. Two experiments were conducted to validate the wear debris imaging feasibility and effectiveness of the newly developed detector. The results show that the visual feature information of the wear debris can be reliably obtained from black oil by this detector, and it can also be used to track the fast-changing wear of tribopairs at different wear stages. To the best of our knowledge, to date there is no other report for solving this issue.

  20. Essential oils of culinary herbs and spices display agonist and antagonist activities at human aryl hydrocarbon receptor AhR.

    PubMed

    Bartoňková, Iveta; Dvořák, Zdeněk

    2018-01-01

    Essential oils (EOs) of culinary herbs and spices are used to flavor, color and preserve foods and drinks. Dietary intake of EOs is significant, deserving an attention of toxicologists. We examined the effects of 31 EOs of culinary herbs and spices on the transcriptional activity of human aryl hydrocarbon receptor (AhR), which is a pivotal xenobiotic sensor, having also multiple roles in human physiology. Tested EOs were sorted out into AhR-inactive ones (14 EOs) and AhR-active ones, including full agonists (cumin, jasmine, vanilla, bay leaf), partial agonists (cloves, dill, thyme, nutmeg, oregano) and antagonists (tarragon, caraway, turmeric, lovage, fennel, spearmint, star anise, anise). Major constituents (>10%) of AhR-active EOs were studied in more detail. We identified AhR partial agonists (carvacrol, ligustilide, eugenol, eugenyl acetate, thymol, ar-turmerone) and antagonists (trans-anethole, butylidine phtalide, R/S-carvones, p-cymene), which account for AhR-mediated activities of EOs of fennel, anise, star anise, caraway, spearmint, tarragon, cloves, dill, turmeric, lovage, thyme and oregano. We also show that AhR-mediated effects of some individual constituents of EOs differ from those manifested in mixtures. In conclusion, EOs of culinary herbs and spices are agonists and antagonists of human AhR, implying a potential for food-drug interactions and interference with endocrine pathways. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Antimicrobial activity of chitosan coatings and films against Listeria monocytogenes on black radish.

    PubMed

    Jovanović, Gordana D; Klaus, Anita S; Nikšić, Miomir P

    2016-01-01

    The antibacterial activity of chitosan coatings prepared with acetic or lactic acid, as well as of composite chitosan-gelatin films prepared with essential oils, was evaluated in fresh shredded black radish samples inoculated with Listeria monocytogenes ATCC 19115 and L. monocytogenes ATCC 19112 during seven days of storage at 4°C. The chitosan coating prepared with acetic acid showed the most effective antibacterial activity. All tested formulations of chitosan films exhibited strong antimicrobial activity on the growth of L. monocytogenes on black radish, although a higher inhibition of pathogens was achieved at higher concentrations of chitosan. The antimicrobial effect of chitosan films was even more pronounced with the addition of essential oils. Chitosan-gelatin films with thyme essential oils showed the most effective antimicrobial activity. A reduction of 2.4log10CFU/g for L. monocytogenes ATCC 19115 and 2.1log10CFU/g for L. monocytogenes ATCC 19112 was achieved in the presence of 1% chitosan film containing 0.2% of thyme essential oil after 24h of storage. Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  2. The impact of oil burning on kraft recovery furnace SO sub 2 emissions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Someshwar, A.V.; Pinkerton, J.E.; Caron, A.L.

    1991-04-01

    Auxiliary fossil fuel, either natural gas or fuel oil, is burned in kraft recovery furnaces during furnace startups and shutdowns, furnace upsets, and periods of substantially reduced rates of black liquor firing. The efficiency of sulfur capture and retention during normal operation of a kraft recovery furnace is inherently high. Consequently, not all the SO{sub 2} from occasional burning of sulfur-containing fuel oil in the furnace would be expected to end up in the stack gases. However, the extent to which such SO{sub 2} is captured by the alkali fume generation processes has not been well documented. In this paper,more » the authors examines the impact that burning oil in kraft recovery furnaces has on the SO{sub 2} emissions. The work included analyses of long-term SO{sub 2} data from a continuous emission monitoring system (CEMS) obtained for four furnaces that burned medium sulfur fuel oil as auxiliary fuel. It also included tests conducted on four furnaces in which varying amounts of oil were co-fired with black liquor.« less

  3. Remote sensing assessment of oil lakes and oil-polluted surfaces at the Greater Burgan oil field, Kuwait

    NASA Astrophysics Data System (ADS)

    Kwarteng, Andy Yaw

    A heinous catastrophe imposed on Kuwait's desert environment during the 1990 to 1991 Arabian Gulf War was the formation of oil lakes and oil-contaminated surfaces. Presently, the affected areas consist of oil lakes, thick light and disintegrated tarmats, black soil and vegetation. In this study, Landsat TM, Spot, colour aerial photographs and IRS-1D digital image data acquired between 1989 and 1998 were used to monitor the spatial and temporal changes of the oil lakes and polluted surfaces at the Greater Burgan oil field. The use of multisensor datasets provided the opportunity to observe the polluted areas in different wavelengths, look angles and resolutions. The images were digitally enhanced to optimize the visual outlook and improve the information content. The data documented the gradual disappearance of smaller oil lakes and soot/black soil from the surface with time. Even though some of the contaminants were obscured by sand and vegetation and not readily observed on the surface or from satellite images, the harmful chemicals still remain in the soil. Some of the contaminated areas displayed a remarkable ability to support vegetation growth during the higher than average rainfall that occurred between 1992 to 1998. The total area of oil lakes calculated from an IRS-1D panchromatic image acquired on 16 February 1998, using supervised classification applied separately to different parts, was 24.13 km 2.

  4. In search of novel highly active mitochondria-targeted antioxidants: thymoquinone and its cationic derivatives.

    PubMed

    Severina, Inna I; Severin, Fedor F; Korshunova, Galina A; Sumbatyan, Natalya V; Ilyasova, Tatyana M; Simonyan, Ruben A; Rogov, Anton G; Trendeleva, Tatyana A; Zvyagilskaya, Renata A; Dugina, Vera B; Domnina, Lidia V; Fetisova, Elena K; Lyamzaev, Konstantin G; Vyssokikh, Mikhail Yu; Chernyak, Boris V; Skulachev, Maxim V; Skulachev, Vladimir P; Sadovnichii, Viktor A

    2013-06-27

    Since the times of the Bible, an extract of black cumin seeds was used as a medicine to treat many human pathologies. Thymoquinone (2-demethylplastoquinone derivative) was identified as an active antioxidant component of this extract. Recently, it was shown that conjugates of plastoquinone and penetrating cations are potent mitochondria-targeted antioxidants effective in treating a large number of age-related pathologies. This review summarizes new data on the antioxidant and some other properties of membrane-penetrating cationic compounds where 2-demethylplastoquinone substitutes for plastoquinone. It was found that such a substitution significantly increases a window between anti- and prooxidant concentrations of the conjugates. Like the original plastoquinone derivatives, the novel compounds are easily reduced by the respiratory chain, penetrate through model and natural membranes, specifically accumulate in mitochondria in an electrophoretic fashion, and strongly inhibit H2O2-induced apoptosis at pico- and nanomolar concentrations in cell cultures. At present, cationic demethylplastoquinone derivatives appear to be the most promising mitochondria-targeted drugs of the quinone series. Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  5. Metabolomics driven analysis of six Nigella species seeds via UPLC-qTOF-MS and GC-MS coupled to chemometrics.

    PubMed

    Farag, Mohamed A; Gad, Haidy A; Heiss, Andreas G; Wessjohann, Ludger A

    2014-05-15

    Nigella sativa, commonly known as black cumin seed, is a popular herbal supplement that contains numerous phytochemicals including terpenoids, saponins, flavonoids, alkaloids. Only a few of the ca. 15 species in the genus Nigella have been characterized in terms of phytochemical or pharmacological properties. Here, large scale metabolic profiling including UPLC-PDA-MS and GC-MS with further multivariate analysis was utilized to classify 6 Nigella species. Under optimized conditions, we were able to annotate 52 metabolites including 8 saponins, 10 flavonoids, 6 phenolics, 10 alkaloids, and 18 fatty acids. Major peaks in UPLC-MS spectra contributing to the discrimination among species were assigned as kaempferol glycosidic conjugates, with kaempferol-3-O-[glucopyranosyl-(1→2)-galactopyranosyl-(1→2)-glucopyranoside, identified as potential taxonomic marker for N. sativa. Compared with GC-MS, UPLC-MS was found much more efficient in Nigella sample classification based on genetic and geographical origin. Nevertheless, both GC-MS and UPLC-MS support the remote position of Nigella nigellastrum in relation to the other taxa. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. A review of effective herbal medicines in controlling menopausal symptoms

    PubMed Central

    Kargozar, Rahele; Azizi, Hoda; Salari, Roshanak

    2017-01-01

    Background Acute menopausal syndrome especially hot flashes, is one of the most common gynecological problems during menopause. Due to the side effects of hormone therapy, herbal and complementary medicines are always of immense interest to people in the treatment and management of the symptoms and complications of menopause. Objective The aim of this study was to investigate the mechanisms and effects of medicinal plants employed in the treatment of menopausal symptoms. Methods This review article was carried out by examining clinical trial studies between the period of 1994 and 2016. The keywords, which include menopause, climacteric, hot flushes, flashes, herb and phytoestrogens were used to search for herbal medicines used in clinical trials for the treatment of menopausal symptoms using databases such as PubMed, Medline, Scopus, Google scholar, SID and Magiran. Results The results of the study showed that the medicinal plants, which include Sage herb (Salvia officinalis), Lemon balm (Melissa officinalis), Valerina officinalis, Black cohosh (Cimicifuga racemosa), Fenugreek (Trigonella foenum-graecum), Black cumin (Nigella sativa), Vitex (Vitex agnus-castus), Fennel (Foeniculum vulgare), Evening primrose (Oenothera biennis), Ginkgo biloba, Alfalfa (Medicago sativa), Hypericum perforatum, Panax ginseng, Pimpinella anisum, Licorice (Glycyrrhiza glabra), Passiflora incarnata, Red clover (Trifolium pratense), and Glycine soja were effective in the treatment of acute menopausal syndrome with different mechanisms. Conclusion Medicinal plants can play an imperative role in the treatment of acute menopausal syndrome; however, further studies are required to buttress their efficacy in the treatment of acute menopausal syndrome. PMID:29403626

  7. Black Gold Revolution: What New Oil Means for American Security Policy

    DTIC Science & Technology

    2015-06-01

    States will keep most or all discovered oil for domestic uses. Incidentally, current laws forbid the export of American oil for this reason. Oil...three prevalent international orders are balance-of-power, hegemonic and constitutional .5 Each of these differs in the distribution of power...peaceful transition, it must consider Gilpin’s concept of prestige to retain influence. This consideration leads to the final order, a constitutional

  8. Eigenvalue-eigenvector decomposition (EED) analysis of dissimilarity and covariance matrix obtained from total synchronous fluorescence spectral (TSFS) data sets of herbal preparations: Optimizing the classification approach

    NASA Astrophysics Data System (ADS)

    Tarai, Madhumita; Kumar, Keshav; Divya, O.; Bairi, Partha; Mishra, Kishor Kumar; Mishra, Ashok Kumar

    2017-09-01

    The present work compares the dissimilarity and covariance based unsupervised chemometric classification approaches by taking the total synchronous fluorescence spectroscopy data sets acquired for the cumin and non-cumin based herbal preparations. The conventional decomposition method involves eigenvalue-eigenvector analysis of the covariance of the data set and finds the factors that can explain the overall major sources of variation present in the data set. The conventional approach does this irrespective of the fact that the samples belong to intrinsically different groups and hence leads to poor class separation. The present work shows that classification of such samples can be optimized by performing the eigenvalue-eigenvector decomposition on the pair-wise dissimilarity matrix.

  9. 49 CFR 172.327 - Petroleum sour crude oil in bulk packaging.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Bulk packaging used to transport petroleum crude oil containing hydrogen sulfide (i.e., sour crude oil... hydrogen sulfide vapors may occur. (b) The border of the square-on-point must be black or red on a white or...., manhole, loading head) where exposure to hydrogen sulfide vapors may occur. The label, tag, or sign must...

  10. 49 CFR 172.327 - Petroleum sour crude oil in bulk packaging.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Bulk packaging used to transport petroleum crude oil containing hydrogen sulfide (i.e., sour crude oil... hydrogen sulfide vapors may occur. (b) The border of the square-on-point must be black or red on a white or...., manhole, loading head) where exposure to hydrogen sulfide vapors may occur. The label, tag, or sign must...

  11. 49 CFR 172.327 - Petroleum sour crude oil in bulk packaging.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Bulk packaging used to transport petroleum crude oil containing hydrogen sulfide (i.e., sour crude oil... hydrogen sulfide vapors may occur. (b) The border of the square-on-point must be black or red on a white or...., manhole, loading head) where exposure to hydrogen sulfide vapors may occur. The label, tag, or sign must...

  12. The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed (Nigella sativa L.) Oil

    PubMed Central

    Mohammed, Nameer Khairullah; Abd Manap, Mohd Yazid; Muhialdin, Belal J.; Alhelli, Amaal M.

    2016-01-01

    The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE. PMID:27642353

  13. The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed (Nigella sativa L.) Oil.

    PubMed

    Mohammed, Nameer Khairullah; Abd Manap, Mohd Yazid; Tan, Chin Ping; Muhialdin, Belal J; Alhelli, Amaal M; Meor Hussin, Anis Shobirin

    2016-01-01

    The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE.

  14. The Effect of Cumin cyminum L. Plus Lime Administration on Weight Loss and Metabolic Status in Overweight Subjects: A Randomized Double-Blind Placebo-Controlled Clinical Trial.

    PubMed

    Taghizadeh, Mohsen; Memarzadeh, Mohammad Reza; Abedi, Fatemeh; Sharifi, Nasrin; Karamali, Fatemeh; Fakhrieh Kashan, Zohreh; Asemi, Zatollah

    2016-08-01

    Limited data are available regarding the effects of combined administration of Cumin cyminum L. and lime on weight loss and metabolic profiles among subjects with overweight subjects. The current study aimed to assess the effects of combined administration of Cumin cyminum L. and lime on weight loss and metabolic profiles among subjects with overweight. This randomized double-blind placebo-controlled clinical trial was conducted on 72 subjects with overweight, aged 18 - 50 years old. Participants were randomly divided into three groups: Group A received high-dose Cumin cyminum L. and lime capsules (75 mg each, n = 24), group B low-dose Cumin cyminum L. and lime capsules (25 mg each, n = 24) and group C placebos (n = 24) twice daily for eight weeks. After eight weeks of intervention, compared with low-dose C. cyminum L. plus lime and placebo, taking high-dose C. cyminum L. plus lime resulted in significant weight loss (in the high-dose group: -2.1 ± 1.7 vs. in the low-dose group: -1.2 ± 1.5 and in the placebo group: + 0.2 ± 1.3 kg, respectively; P < 0.001) and body mass index (-0.8 ± 0.6 vs. -0.5 ± 0.5 and +0.1 ± 0.5 kg/m 2 , respectively; P < 0.001). In addition, administration of high-dose C. cyminum L. plus lime compared with low-dose C. cyminum L. plus lime and placebo, led to a significant reduction in fasting plasma glucose (FPG) (P < 0.001) and a significant rise in quantitative insulin sensitivity check index (QUICKI) (+ 0.02 ± 0.02 vs. + 0.01 ± 0.02 and 0.01 ± 0.01, respectively; P = 0.01). Moreover, a significant decrease in serum triglycerides (-14.1 ± 56.2 vs. +13.9 ± 36.8 and + 10.6 ± 25.1 mg/dL; respectively; P = 0.03), total-cholesterol (-18.4 ± 28.6 vs. +8.6 ± 28.5 and -1.0 ± 24.8 mg/dL; respectively; P = 0.004) and low density lipoproteins- (LDL)-cholesterol levels (-11.8 ± 20.7 vs. +6.5 ± 23.2 and -2.9 ± 20.4 mg/dL, respectively; P = 0.01) was observed following the consumption of high-dose C. cyminum L. plus lime compared with low-dose C. cyminum L. plus lime and placebo. Results of the current study indicated that taking high-dose C. cyminum L. plus lime for eight weeks among subjects with overweight had beneficial effects on weight, BMI, FPG, QUICKI, triglycerides, total-cholesterol and LDL-cholesterol levels.

  15. The Effect of Cumin cyminum L. Plus Lime Administration on Weight Loss and Metabolic Status in Overweight Subjects: A Randomized Double-Blind Placebo-Controlled Clinical Trial

    PubMed Central

    Taghizadeh, Mohsen; Memarzadeh, Mohammad Reza; Abedi, Fatemeh; Sharifi, Nasrin; Karamali, Fatemeh; Fakhrieh Kashan, Zohreh; Asemi, Zatollah

    2016-01-01

    Background Limited data are available regarding the effects of combined administration of Cumin cyminum L. and lime on weight loss and metabolic profiles among subjects with overweight subjects. Objectives The current study aimed to assess the effects of combined administration of Cumin cyminum L. and lime on weight loss and metabolic profiles among subjects with overweight. Patients and Methods This randomized double-blind placebo-controlled clinical trial was conducted on 72 subjects with overweight, aged 18 - 50 years old. Participants were randomly divided into three groups: Group A received high-dose Cumin cyminum L. and lime capsules (75 mg each, n = 24), group B low-dose Cumin cyminum L. and lime capsules (25 mg each, n = 24) and group C placebos (n = 24) twice daily for eight weeks. Results After eight weeks of intervention, compared with low-dose C. cyminum L. plus lime and placebo, taking high-dose C. cyminum L. plus lime resulted in significant weight loss (in the high-dose group: -2.1 ± 1.7 vs. in the low-dose group: -1.2 ± 1.5 and in the placebo group: + 0.2 ± 1.3 kg, respectively; P < 0.001) and body mass index (-0.8 ± 0.6 vs. -0.5 ± 0.5 and +0.1 ± 0.5 kg/m2, respectively; P < 0.001). In addition, administration of high-dose C. cyminum L. plus lime compared with low-dose C. cyminum L. plus lime and placebo, led to a significant reduction in fasting plasma glucose (FPG) (P < 0.001) and a significant rise in quantitative insulin sensitivity check index (QUICKI) (+ 0.02 ± 0.02 vs. + 0.01 ± 0.02 and 0.01 ± 0.01, respectively; P = 0.01). Moreover, a significant decrease in serum triglycerides (-14.1 ± 56.2 vs. +13.9 ± 36.8 and + 10.6 ± 25.1 mg/dL; respectively; P = 0.03), total-cholesterol (-18.4 ± 28.6 vs. +8.6 ± 28.5 and -1.0 ± 24.8 mg/dL; respectively; P = 0.004) and low density lipoproteins- (LDL)-cholesterol levels (-11.8 ± 20.7 vs. +6.5 ± 23.2 and -2.9 ± 20.4 mg/dL, respectively; P = 0.01) was observed following the consumption of high-dose C. cyminum L. plus lime compared with low-dose C. cyminum L. plus lime and placebo. Conclusions Results of the current study indicated that taking high-dose C. cyminum L. plus lime for eight weeks among subjects with overweight had beneficial effects on weight, BMI, FPG, QUICKI, triglycerides, total-cholesterol and LDL-cholesterol levels. PMID:27781121

  16. Effects of different black mediators on the shear strength of orthodontic bracket to the enamel treated with Nd-Yag laser

    NASA Astrophysics Data System (ADS)

    Huang, Shun-Te; Lin, I.-Shueng; Tsai, Chi-Cheng

    1995-04-01

    The Nd:YAG laser has ablation, crack, and crater effects on the dental enamel through black mediators which are very similar to the acid etching effects of phosphoric acid. This study was designed for searching how the different black mediators influence the shear strengths of the brackets bound to the enamel surfaces which were treated with the Nd:YAG laser. 90 bovine enamels divided into 5 groups were painted with 5 kinds of black mediators including Chinese ink, oil ink, black ball pen, water ink and black transfer paper. The enamel surfaces painted with black mediators were then radiated by Nd:YAG laser (ADL; American Dental Laser 300dl, power: 20 pps, 87.5 mj). Orthodontic brackets were bonded to the radiated surfaces. Then the shear strengths of the brackets to the enamels were measured by Instron. The results showed that the Chinese ink group and oil ink group has the strongest shear strength, ball pen group and water ink group showed the second strength, and the transfer paper group has the lowest shear strength. In addition, scanning electronic microscope also was used to observe the topographic changes of the enamel surfaces induced by the laser ablation.

  17. Effect of storage on the essential oil composition of Piper nigrum L. fruits of different ripening states.

    PubMed

    Orav, Anne; Stulova, Irina; Kailas, Tiiu; Müürisepp, Mati

    2004-05-05

    The qualitative and quantitative composition of the essential oil from black, green, and white pepper was determined by using a simultaneous distillation and extraction micromethod for oil isolation and gas chromatography (GC)/flame ionization detection (FID) and GC/mass spectrometry (MS) analysis techniques. The most abundant compounds in pepper oils were (E)-beta-caryophyllene (1.4-70.4%), limonene (2.9-38.4%), beta-pinene (0.7-25.6%), Delta-3-carene (1.7-19.0%), sabinene (0-12.2%), alpha-pinene (0.3-10.4%), eugenol (0.1-41.0%), terpinen-4-ol (0-13.2%), hedycaryol (0-9.1%), beta-eudesmol (0-9.7%), and caryophyllene oxide (0.1-7.2%). Green pepper corn obtained by a sublimation drying method gave more oil (12.1 mg/g) and a much higher content of monoterpenes (84.2%) in the oil than air-dried green pepper corn (0.8 mg/g and 26.8%, respectively). The oil from ground black pepper contained more monoterpenes and less sesquiterprnes and oxygenated terpenoids as compared to green and white pepper oils. After 1 year of storage of pepper samples in a glass vessel at room temperature, the amount of the oils isolated decreased, the content of terpenes decreased, and the amount of oxygenated terpenoids increased. Differently from other pepper samples, 1 year storage of green pepper corn raised the oil amount more than twice of both drying methods.

  18. Eigenvalue-eigenvector decomposition (EED) analysis of dissimilarity and covariance matrix obtained from total synchronous fluorescence spectral (TSFS) data sets of herbal preparations: Optimizing the classification approach.

    PubMed

    Tarai, Madhumita; Kumar, Keshav; Divya, O; Bairi, Partha; Mishra, Kishor Kumar; Mishra, Ashok Kumar

    2017-09-05

    The present work compares the dissimilarity and covariance based unsupervised chemometric classification approaches by taking the total synchronous fluorescence spectroscopy data sets acquired for the cumin and non-cumin based herbal preparations. The conventional decomposition method involves eigenvalue-eigenvector analysis of the covariance of the data set and finds the factors that can explain the overall major sources of variation present in the data set. The conventional approach does this irrespective of the fact that the samples belong to intrinsically different groups and hence leads to poor class separation. The present work shows that classification of such samples can be optimized by performing the eigenvalue-eigenvector decomposition on the pair-wise dissimilarity matrix. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Turkey Country Analysis Brief

    EIA Publications

    2017-01-01

    Turkey is well placed to serve as a hub for oil and natural gas supply headed to Europe and other Atlantic markets from Russia, the Caspian region, and the Middle East. Turkey has been a major transit point for oil and is becoming more important as a transit point for natural gas. Growing volumes of Caspian oil are being sent to Black Sea ports such as Novorossisyk, Russia and Supsa, Georgia and then to Western markets by tanker via the Turkish Straits (Bosporus and Dardanelles waterways). Caspian oil and oil from northern Iraq also cross Turkey by pipeline, through the Ceyhan oil terminal on Turkey's Mediterranean coast.

  20. Evaluation of various agro-wastes for traditional black soap production.

    PubMed

    Taiwo, O E; Osinowo, F A

    2001-08-01

    The agricultural wastes, cocoa-pod husks, palm-bunch waste, sorghum chaff and groundnut shells, which are normally thrown away have been used in the production of black soap. Unlike other soaps which are made from oils and chemicals, black soap is made from oils and agro-wastes ashes. Chemical analysis indicated that the liquid extract from the ashes of the different agro-wastes used contained various amounts of potassium and sodium compounds. The most common ingredient in the agro-wastes was potassium carbonate. The amount of potassium carbonate was 56.73 +/- 0.16% in cocoa-pod ash, 43.15 +/- 0.13% in palm-bunch ash, 16.65 +/- 0.05% in groundnut shell ash and 12.40 +/- 0.08% in sorghum chaff ash. Soaps made from the agro-wastes ashes had excellent solubility, consistency, cleansing and lathering abilities.

  1. Fate and Effects of Crude Oil Spilled on Subarctic Permafrost Terrain in Interior Alaska: Fifteen Years Later

    DTIC Science & Technology

    1993-08-01

    tundra ecosystems of Alas- (75 m2) having surface oil visible. In contrast, most ka and northern Canada (Deneke et al. 1974, Atlas of the crude oil...Overall soil microbial activity was growing black spruce (Picea mariana) forest with an increased, with some components of the microbial understory of...terrestrial environments, in the heavily affected portions of the site; they 3. Determine the effect of crude oil spills on soil found that microbial

  2. Sacrificial adsorbate for surfactants utilized in chemical floods of enhanced oil recovery operations

    DOEpatents

    Johnson, Jr., James S.; Westmoreland, Clyde G.

    1982-01-01

    The present invention is directed to a sacrificial or competitive adsorbate for surfactants contained in chemical flooding emulsions for enhanced oil recovery operations. The adsorbate to be utilized in the method of the present invention is a caustic effluent from the bleach stage or the weak black liquor from the digesters and pulp washers of the kraft pulping process. This effluent or weak black liquor is injected into an oil-bearing subterranean earth formation prior to or concurrent with the chemical flood emulsion and is adsorbed on the active mineral surfaces of the formation matrix so as to effectively reduce adsorption of surfactant in the chemical flood. Alternatively, the effluent or liquor can be injected into the subterranean earth formation subsequent to a chemical flood to displace the surfactant from the mineral surfaces for the recovery thereof.

  3. Sacrificial adsorbate for surfactants utilized in chemical floods of enhanced oil recovery operations

    DOEpatents

    Johnson, J.S. Jr.; Westmoreland, C.G.

    1980-08-20

    The present invention is directed to a sacrificial or competitive adsorbate for surfactants contained in chemical flooding emulsions for enhanced oil recovery operations. The adsorbate to be utilized in the method of the present invention is a caustic effluent from the bleach stage or the weak black liquor from the digesters and pulp washers of the kraft pulping process. This effluent or weak black liquor is injected into an oil-bearing subterranean earth formation prior to or concurrent with the chemical flood emulsion and is adsorbed on the active mineral surfaces of the formation matrix so as to effectively reduce adsorption of surfactant in the chemical flood. Alternatively, the effluent or liquor can be injected into the subterranean earth formation subsequent to a chemical flood to displace the surfactant from the mineral surfaces for the recovery thereof.

  4. Isolation and characterization of formacell Lignins from oil empty fruits bunches

    NASA Astrophysics Data System (ADS)

    Hidayati, S.; Zuidar, A. S.; Satyajaya, W.; Murhadi; Retnowati, D.

    2018-04-01

    Lignin is the largest component in black liquor, it is about 46% of solids total and can be isolated by precipitation using acid and base method. The purpose of this study was to get the best NaOH concentration to produce lignin with yield, solids total content, metoxyle lignins content, weights equivalent of lignin in the black liquor by pulping formacell process from oil empty fruits bunches. This study was done with isolation lignin process in black liquor used by NaOH concentration were 5%, 10%, 15%, 20%, 25%, and 30% from volume black liquor and then precipitationed for 10 hours. The result of this research showed the isolation of lignin with NaOH concentration 30% get the pH 5,42%, yield of lignin was 5,67%, solids black liquor total was 65,11%, levels of metoxyle lignin 14,61%, and equivalent weights of lignin was 1787,23. The result of FT-IR identifications of isolates lignin in NaOH concentration 25 and 30% showed a pattern infiltration spektro IR that almost a part that have the same infiltration at the wave numbers that showed lignin had one of the rings lignin was guaiasil, it was building blocks of non wood lignin.

  5. Low-fat papadams from black gram-tapioca blends.

    PubMed

    Annapure, U S; Michael, M; Singhal, R S; Kulkarni, P R

    1997-07-01

    Papadams, made from black gram (Phaseolus mungo) and largely manufactured on cottage scale are popular in the Indian dietary. Escalating prices of black gram coupled with abundant availability of tapioca flour at almost 1/8th the price of black gram prompted the study of papadam characteristics using blends of black gram and tapioca flour. Tapioca flour up to 25% substitution did not alter the sensory attributes. However, the expansibility of the product decreased on addition of tapioca flour; a fact which could be overcome by using carboxymethyl cellulose (CMC) at 3% level of black gram flour. This decreased the oil content to 19.7% and also increased the expansibility to 5.539%.

  6. [Inhibition of Linseed Oil Autooxidation by Essential Oils and Extracts from Spice Plants].

    PubMed

    Misharina, T A; Alinkina, E S; Terenina, M B; Krikunova, N I; Kiseleva, V I; Medvedeva, I B; Semenova, M G

    2015-01-01

    Clove bud essential oil, extracts from ginger, pimento and black pepper, or ascorbyl palmytate were studied as natural antioxidants for the inhibition of autooxidation of polyunsaturated fatty acids in linseed oil. Different methods were used to estimate antioxidant efficiency. These methods are based on the following parameters: peroxide values; peroxide concentration; content of degradation products of unsaturated fatty acid peroxides, which acted with thiobarbituric acid; diene conjugate content; the content of volatile compounds that formed as products of unsaturated fatty acid peroxide degradation; and the composition of methyl esters of fatty acids in samples of oxidized linseed oil.

  7. Advanced direct coal liquefaction concepts. Quarterly report, July 1--September 30, 1994

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Berger, D.J.; Parker, R.J.; Simpson, P.L.

    All the reports required for completion of the contract were submitted this quarter. A proposal for further work in Phase 2 was also submitted. The technical and economic assessment of the process was completed. The results show that for the base case scenario (25% equity, 15% after tax DCF-ROE) coal derived synthetic crude oil can be produced at just below US $30 per barrel. The study was based on the production of 75,000 BPD of C{sub 4+} synthetic crude oil from Black Thunder coal for subsequent processing in a conventional petroleum refinery from Black Thunder (Wyoming) subbituminous coal.

  8. Advanced direct coal liquefaction concepts. Quarterly report, January 1, 1994--March 31, 1994

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Berger, D.J.; Parker, R.J.; Simpson, P.L.

    A detailed evaluation of the bench unit data on Black Thunder feedstocks was completed. The results show that in a once-through operation using counterflow, reactor technology coal conversions in excess of 90% could be obtained, giving distillable oil yields in the range 60--65 wt % on MAF coal. The remaining non-distillable oil fraction which represents 20--25 wt % on MAF coal is a source of additional distillable oil in further processing, for example, bottoms recycle operation. C{sub 1}-C{sub 3} gas yields were generally in the order of 6--8 wt %. In autoclave studies, Illinois No. 6 coal was found tomore » be much less reactive than Black Thunder coal, and did not respond well to solubilization with carbon monoxide/steam. Process severity was, therefore, increased for bench unit operations on Illinois No. 6 coal, and work has concentrated on the use of hydrogen rather than carbon monoxide for solubilization. Preliminary coking studies on the resid from bench unit runs on Black Thunder coal were also carried out. Distillable liquid yields of 55--60 wt % were obtained. The technical and economic study to be carried out by Kilborn Engineering Company has been initiated.« less

  9. Incidence of Escherichia coli in black walnut meats.

    PubMed

    Meyer, M T; Vaughn, R H

    1969-11-01

    Examination of commercially shelled black walnut meats showed inconsistent numbers of total aerobic bacteria, coliforms, and Escherichia coli; variation occurred among different meat sizes and within each meat size. The incidence of E. coli on meats of commercially hulled black walnuts depended on the physical condition of the nuts. Apparently tightly sealed ones contained only a few or none, whereas those with visibly separated sutures and spoiled meats yielded the most. This contamination was in part correlated to a hulling operation. Large numbers of E. coli on the husk of the walnuts contaminated the hulling water, subsequently also contaminating the meats by way of separated sutures. Chlorination of the hulling wash water was ineffective. Attempts were made to decontaminate the walnut meats without subsequent deleterious changes in flavor or texture. A treatment in coconut oil at 100 C followed by removal of excess surface oil by centrifugation was best.

  10. Incidence of Escherichia coli in Black Walnut Meats

    PubMed Central

    Meyer, Melvin T.; Vaughn, Reese H.

    1969-01-01

    Examination of commercially shelled black walnut meats showed inconsistent numbers of total aerobic bacteria, coliforms, and Escherichia coli; variation occurred among different meat sizes and within each meat size. The incidence of E. coli on meats of commercially hulled black walnuts depended on the physical condition of the nuts. Apparently tightly sealed ones contained only a few or none, whereas those with visibly separated sutures and spoiled meats yielded the most. This contamination was in part correlated to a hulling operation. Large numbers of E. coli on the husk of the walnuts contaminated the hulling water, subsequently also contaminating the meats by way of separated sutures. Chlorination of the hulling wash water was ineffective. Attempts were made to decontaminate the walnut meats without subsequent deleterious changes in flavor or texture. A treatment in coconut oil at 100 C followed by removal of excess surface oil by centrifugation was best. PMID:4905608

  11. Finding Novel Antibiotic Substances from Medicinal Plants – Antimicrobial Properties of Nigella Sativa Directed against Multidrug-resistant Bacteria

    PubMed Central

    Bakal, Seher Nancy; Bereswill, Stefan; Heimesaat, Markus M.

    2017-01-01

    The progressive rise in multidrug-resistant (MDR) bacterial strains poses serious problems in the treatment of infectious diseases. While the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. This rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. The medicinal herb Nigella sativa and its derivatives constitute promising candidates. In a comprehensive literature survey (using the PubMed data base), we searched for publications on the antimicrobial effects of N. sativa particularly directed against MDR bacterial strains. In vitro studies published between 2000 and 2015 revealed that N. sativa exerted potent antibacterial effects against both Gram-positive and Gram-negative species including resistant strains. For instance, N. sativa inhibited the growth of bacteria causing significant gastrointestinal morbidity such as Salmonella, Helicobacter pylori, and Escherichia coli. However, Listeria monocytogenes and Pseudomonas aeruginosa displayed resistance against black cumin seed extracts. In conclusion, our literature survey revealed potent antimicrobial properties of N. sativa against MDR strains in vitro that should be further investigated in order to develop novel therapeutic perspectives for combating infectious diseases particularly caused by MDR strains. PMID:28386474

  12. Biased activity of soluble guanylyl cyclase: the Janus face of thymoquinone.

    PubMed

    Detremmerie, Charlotte; Vanhoutte, Paul M; Leung, Susan

    2017-07-01

    The natural compound thymoquinone, extracted from Nigella sativa (black cumin), is widely used in humans for its anti-oxidative properties. Thymoquinone is known for its acute endothelium-independent vasodilator effects in isolated rat aortae and pulmonary arteries, depending in part on activation of adenosine triphosphate-sensitive potassium channels and inhibition of voltage-dependent calcium channels. The compound also improves endothelial dysfunction in mesenteric arteries of ageing rodents and in aortae of rabbits treated with pyrogallol, by inhibiting oxidative stress. Serendipitously, thymoquinone was found to augment contractions in isolated arteries with endothelium of both rats and pigs. The endothelium-dependent augmentation it causes counterintuitively depends on biased activation of soluble guanylyl cyclase (sGC) producing inosine 3',5'-cyclic monophosphate (cyclic IMP) rather than guanosine 3',5'-cyclic monophosphate. This phenomenon shows a striking mechanistic similarity to the hypoxic augmentation previously observed in porcine coronary arteries. The cyclic IMP preferentially produced under thymoquinone exposure causes an increased contractility of arterial smooth muscle by interfering with calcium homeostasis. This brief review summarizes the vascular pharmacology of thymoquinone, focussing in particular on how the compound causes endothelium-dependent contractions by biasing the activity of sGC.

  13. Finding Novel Antibiotic Substances from Medicinal Plants - Antimicrobial Properties of Nigella Sativa Directed against Multidrug-resistant Bacteria.

    PubMed

    Bakal, Seher Nancy; Bereswill, Stefan; Heimesaat, Markus M

    2017-03-01

    The progressive rise in multidrug-resistant (MDR) bacterial strains poses serious problems in the treatment of infectious diseases. While the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. This rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. The medicinal herb Nigella sativa and its derivatives constitute promising candidates. In a comprehensive literature survey (using the PubMed data base), we searched for publications on the antimicrobial effects of N. sativa particularly directed against MDR bacterial strains. In vitro studies published between 2000 and 2015 revealed that N. sativa exerted potent antibacterial effects against both Gram-positive and Gram-negative species including resistant strains. For instance, N. sativa inhibited the growth of bacteria causing significant gastrointestinal morbidity such as Salmonella, Helicobacter pylori , and Escherichia coli . However, Listeria monocytogenes and Pseudomonas aeruginosa displayed resistance against black cumin seed extracts. In conclusion, our literature survey revealed potent antimicrobial properties of N. sativa against MDR strains in vitro that should be further investigated in order to develop novel therapeutic perspectives for combating infectious diseases particularly caused by MDR strains.

  14. Penile erection responses of Nigella sativa seed extract on isolated rat corpus cavernosum

    NASA Astrophysics Data System (ADS)

    Aminyoto, M.; Ismail, S.

    2018-04-01

    Nigella sativa L. (NS) from Ranunculaceae family is known as black cumin in Indonesia. The seed has been used as an aphrodisiac in ethnobotanical studies and reported to have pharmacological activities such as antihypertensive through the relaxant effect of vascular smooth muscles but the direct effect to the blood vessels of the corpus cavernosum is still unknown. The purpose of this study was to examine the response of NS seed extract on penile erection in vitro. NS seeds were macerated in ethanol solvent for three days in room temperature and repeated for two times. Penile erection responses was assessed using isolated rat corpus cavernosum in Krebs-Henseleit solution, temperature 37°C, pH 7.4, aerated with carbogen gas. After acclimation, corpus cavernosum was contracted with a phenylephrine solution. Ethanolic extract of NS seeds or control solution were given after reaching the plateu phase of the highest contraction. This study showed that the contraction response of the corpus cavernosum decreased after addition of NS extract and this action was increased with the addition of the extract concentration. This study concluded that NS seed ethanol extract affects the penile erection response directly through the relaxation of blood vessels in the corpus cavernosum.

  15. Arsenic, cadmium, chromium, lead, manganese, mercury, and selenium in feathers of Black-legged Kittiwake (Rissa tridactyla) and Black Oystercatcher (Haematopus bachmani) from Prince William Sound, Alaska

    PubMed Central

    Burger, Joanna; Gochfeld, Michael; Sullivan, Kelsey; Irons, David; McKnight, Aly

    2014-01-01

    Arsenic, cadmium, chromium, lead, manganese, mercury and selenium were analyzed in the feathers of Black-legged Kittiwakes (Rissa tridactyla) from Shoup Bay in Prince William Sound, Alaska to determine if there were age-related differences in metal levels, and in Black Oystercatchers (Haematopus bachmani)) from the same region to determine if there were differences in oiled and unoiled birds. Except for mercury, there were no age-related differences in metals levels in the feathers of kittiwakes. Kittiwakes over 13 years of age had the highest levels of mercury. There were no differences in levels of metals in the feathers of oystercatchers from oiled and unoiled regions of Prince William Sound. Except for mercury, the feathers of oystercatchers had significantly higher levels of all metals than those of kittiwakes. Levels of mercury in kittiwake feathers (mean of 2910 ng/g [ppb]) were within the range of many species of seabirds reported for other studies, and were generally below adverse effects levels. PMID:18440597

  16. Petroleum geology of Azov-Black Sea region

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lukin, A.; Trofimenko, G.

    1995-08-01

    The main features of tectonics, stratigraphy, paleogeography, lithology, hydrogeology, geothermics and hydrocarbon-bearingness of Azov-Black Sea Region are characterized on the basis of present-day data. Among the most prospective petroliferous complexes one ought to mention: Paleozoic (S - D - C{sub 1}) of Near-Dobrudga foredeep, Triassic - Jurassic of the Black Sea (shelf and continental slope); Lower Cretaceous of the various parts of the Region; Upper Cretaceous of the Black Sea shelf; Paleocene-Eocene of Azov Sea. In addition certain prospects are connected with Precambrian and Paleozoic basements within conjunction zone between Eastern-Europe platform and Scythian plate. Geodynamic evolution of the Regionmore » is considered with determination of tension and compression stages and characteristic of the main regularities of diapirs, mud volcanos, swells, horsts and grabens distribution. There determined the most interesting types of hydrocarbon traps connected with various tectonic forms, river and deltaic channels, bars, conturites, carbonate reefs, etc. Paleogeothermic and paleogeodynamic reconstructions allow to determine the main phases of oil and gas accumulation. The most prospective oil-gas-bearing zones and areas are mapped.« less

  17. Profile of Polyphenolic and Essential Oil Composition of Polish Propolis, Black Poplar and Aspens Buds.

    PubMed

    Okińczyc, Piotr; Szumny, Antoni; Szperlik, Jakub; Kulma, Anna; Franiczek, Roman; Żbikowska, Beata; Krzyżanowska, Barbara; Sroka, Zbigniew

    2018-05-25

    In this work, we studied similarities and differences between 70% ethanol in water extract (70EE) and essential oils (EOs) obtained from propolis, black poplars ( Populus nigra L.) and aspens ( P. tremula L.) to ascertain which of these is a better indicator of the plant species used by bees to collect propolis precursors. Composition of 70EE was analyzed by UPLC-PDA-MS, while GC-MS was used to research the EOs. Principal component analyses (PCA) and calculations of Spearman's coefficient rank were used for statistical analysis. Statistical analysis exhibited correlation between chemical compositions of propolis and Populus buds' 70EE. In the case of EOs, results were less clear. Compositions of black poplars, aspens EOs and propolises have shown more variability than 70EE. Different factors such as higher instability of EOs compared to 70EE, different degradation pattern of benzyl esters to benzoic acid, differences in plant metabolism and bees' preferences may be responsible for these phenomena. Our research has therefore shown that 70EE of propolis reflected the composition of P. nigra or complex aspen⁻black poplar origin.

  18. Tall oil precursors of Douglas fir

    Treesearch

    Daniel O. Foster; Duane F. Zinkel; Anthony H. Conner

    1980-01-01

    The sapwood and heartwood extractives of Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] and the tall oil in the kraft black liquor were characterized. On pulping, isomerization and conversion of conjugated resin acids to dehydroabietic acid was observed. Recovery of both fatty and resin acids from pulping was lower than predicted from the extractive composition....

  19. 33 CFR 157.08 - Applicability of subpart B.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... carries 200 cubic meters or more of crude oil or products in bulk as cargo, as well as to each oceangoing... tanker, that carries less than 1,000 cubic meters of crude oil or products in bulk as cargo and which... black feedstock, or other products with similar physical properties, such as specific gravity and...

  20. 33 CFR 157.08 - Applicability of subpart B.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... carries 200 cubic meters or more of crude oil or products in bulk as cargo, as well as to each oceangoing... tanker, that carries less than 1,000 cubic meters of crude oil or products in bulk as cargo and which... black feedstock, or other products with similar physical properties, such as specific gravity and...

  1. 33 CFR 157.08 - Applicability of subpart B.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... carries 200 cubic meters or more of crude oil or products in bulk as cargo, as well as to each oceangoing... tanker, that carries less than 1,000 cubic meters of crude oil or products in bulk as cargo and which... black feedstock, or other products with similar physical properties, such as specific gravity and...

  2. 33 CFR 157.08 - Applicability of subpart B.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... carries 200 cubic meters or more of crude oil or products in bulk as cargo, as well as to each oceangoing... tanker, that carries less than 1,000 cubic meters of crude oil or products in bulk as cargo and which... black feedstock, or other products with similar physical properties, such as specific gravity and...

  3. 33 CFR 157.08 - Applicability of subpart B.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... carries 200 cubic meters or more of crude oil or products in bulk as cargo, as well as to each oceangoing... tanker, that carries less than 1,000 cubic meters of crude oil or products in bulk as cargo and which... black feedstock, or other products with similar physical properties, such as specific gravity and...

  4. Analysis of Degree of Similarity among Crude Oils, the Upper and the Lower Crust, Organic Matter, Clays, and Different Caustobioliths by the Content of Their Main and Trace Elements

    NASA Astrophysics Data System (ADS)

    Rodkin, Mikhail; Punanova, Svetlana

    2016-04-01

    The goal of this research was to estimate, based on the content of Trace Elements, the level of contribution of the lower and the upper crust as well as the organic matter into ontogenesis of hydrocarbons. The analysis of degree of similarity of the main and trace element (TE) content among the upper and lower continental crust, clays, organic matter, and different caustobioliths (oil, coal, oil-and-black shales) is performed by calculating coefficients of correlation of logarithms of concentrations of a large number of different chemical elements. Different oils from a number of oil bearing provinces in Russia and from the volcanic caldera Uzon (Kamchatka, Russia) were examined. It has been shown that the content of main elements and TEs of coals and oil-and-black shales is better correlated with the chemical composition of the upper crust, while the TE content of oils correlates better with the composition of the lower continental crust. The TE content of oils correlates with the chemical content of living organisms but the correlation in the most cases is weaker than the one with the lower crust. The results of the examination of different samples from the same oil-bearing province were found to be similar. The mean results for different oil-bearing provinces can vary considerably. The results of the examination of young oil from the Uzon volcanic caldera were found to be rather specific and different from the other oils. We also suggest a set of a small number of "characteristic" elements (Cs, Rb, K, U, V, Cr and Ni), which allows to compare the degree of similarity between an oil sample and upper or lower continental crust using only a few chemical elements. Some interpretation of the results is presented.

  5. Pulverizing processes affect the chemical quality and thermal property of black, white, and green pepper (Piper nigrum L.).

    PubMed

    Liu, Hong; Zheng, Jie; Liu, Pengzhan; Zeng, Fankui

    2018-06-01

    In this study, the effects of different pulverizing methods on the chemical attributes and thermal properties of black, white and green pepper were evaluated. Cryogenic grinding minimally damaged the lipid, moisture, crude protein, starch, non-volatile ether extract, piperine, essential oil and the typical pepper essential oil compounds of the spices. The pulverizing methods and storage significantly affected the compositions of the fatty acid in the peppers, except for palmitic acid and lignoceric acid. The amino acid contents and the thermo-gravimetric analysis curve were hardly influenced by the grinding techniques. The use of cryogenic grinding to prepare pepper ensured the highest quality of pepper products. Regardless of grinding technique, the values of moisture, piperine, unsaturated fatty acids, essential oil, monoterpenes, and the absolute concentrations of typical pepper essential oil constituents (except caryophyllene oxide) decreased, whereas the amino acid, lipid, protein, starch, and non-volatile ether extract content as well as the thermal properties were insignificantly changed after storage at 4 °C for 6 months.

  6. Hydrodynamic modeling of petroleum reservoirs using simulator MUFITS

    NASA Astrophysics Data System (ADS)

    Afanasyev, Andrey

    2015-04-01

    MUFITS is new noncommercial software for numerical modeling of subsurface processes in various applications (www.mufits.imec.msu.ru). To this point, the simulator was used for modeling nonisothermal flows in geothermal reservoirs and for modeling underground carbon dioxide storage. In this work, we present recent extension of the code to petroleum reservoirs. The simulator can be applied in conventional black oil modeling, but it also utilizes a more complicated models for volatile oil and gas condensate reservoirs as well as for oil rim fields. We give a brief overview of the code by providing the description of internal representation of reservoir models, which are constructed of grid blocks, interfaces, stock tanks as well as of pipe segments and pipe junctions for modeling wells and surface networks. For conventional black oil approach, we present the simulation results for SPE comparative tests. We propose an accelerated compositional modeling method for sub- and supercritical flows subjected to various phase equilibria, particularly to three-phase equilibria of vapour-liquid-liquid type. The method is based on the calculation of the thermodynamic potential of reservoir fluid as a function of pressure, total enthalpy and total composition and storing its values as a spline table, which is used in hydrodynamic simulation for accelerated PVT properties prediction. We provide the description of both the spline calculation procedure and the flashing algorithm. We evaluate the thermodynamic potential for a mixture of two pseudo-components modeling the heavy and light hydrocarbon fractions. We develop a technique for converting black oil PVT tables to the potential, which can be used for in-situ hydrocarbons multiphase equilibria prediction under sub- and supercritical conditions, particularly, in gas condensate and volatile oil reservoirs. We simulate recovery from a reservoir subject to near-critical initial conditions for hydrocarbon mixture. We acknowledge financial support by a Grant from the president of the Russian Federation (SP-2222.2012.5) and by Russian foundation for basic research (RFBR 15-31-20585).

  7. Herbal infusions of black seed and wheat germ oil: Their chemical profiles, in vitro bio-investigations and effective formulations as Phyto-Nanoemulsions.

    PubMed

    Gumus, Z Pinar; Guler, Emine; Demir, Bilal; Barlas, F Baris; Yavuz, Murat; Colpankan, Dilara; Senisik, A Murat; Teksoz, Serap; Unak, Perihan; Coskunol, Hakan; Timur, Suna

    2015-09-01

    The reported studies related to black seed oil (BSO) and wheat germ oil (WGO) have illustrated that they have a wide range of biological activities. Therefore, enhancing the amount of bio-active compounds that caused higher cell based anti-oxidative effect as well as cell proliferation, etc. in seed oils, infusion of crude plant material has been gained importance as a traditional technique. Herein, we accomplished the infusion of Calendula flowers that also contains many phyto-constituents into BSO and WGO. After the infusion of oils, the change of phytochemical amount was investigated and evaluated according to the oils by chromatography, radical scavenging activity. Subsequently, for investigating the biological impact upon live cells, cytotoxicity, cell-based antioxidant capacity, wound healing and radioprotective activity were tested with monkey kidney fibroblast like cells (Vero) and HaCaT keratinocytes. In vitro cell based experiments (wound healing and radioprotective activity) confirmed that Calendula infused BSO and WGO have greater bio-activity when compared to those plain forms. The herbal oils prepared with an effective extraction technique were incorporated into nanoemulsion systems which will be then called as 'Phyto-Nanoemulsion'. After herbal oil biomolecules were encapsulated into nanoemulsion based delivery systems, the designed formulations were investigated in terms of biological activities. In conclusion, these preparations could be a good candidate as a part of dermal cosmetic products or food supplements which have the therapeutic efficiency, especially after radio- or chemotherapy. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Development of Oil Spill Monitoring System for the Black Sea, Caspian Sea and the Barents/Kara Seas (DEMOSS)

    NASA Astrophysics Data System (ADS)

    Sandven, Stein; Kudriavtsev, Vladimir; Malinovsky, Vladimir; Stanovoy, Vladimir

    2008-01-01

    DEMOSS will develop and demonstrate elements of a marine oil spill detection and prediction system based on satellite Synthetic Aperture Radar (SAR) and other space data. In addition, models for prediction of sea surface pollution drift will be developed and tested. The project implements field experiments to study the effect of artificial crude oil and oil derivatives films on short wind waves and multi-frequency (Ka-, Ku-, X-, and C-band) dual polarization radar backscatter power and Doppler shift at different wind and wave conditions. On the basis of these and other available experimental data, the present model of short wind waves and radar scattering will be improved and tested.A new approach for detection and quantification of the oil slicks/spills in satellite SAR images is developed that can discriminate human oil spills from biogenic slicks and look-alikes in the SAR images. New SAR images are obtained in coordination with the field experiments to test the detection algorithm. Satellite SAR images from archives as well as from new acquisitions will be analyzed for the Black/Caspian/Kara/Barents seas to investigate oil slicks/spills occurrence statistics.A model for oil spills/slicks transport and evolution is developed and tested in ice-infested arctic seas, including the Caspian Sea. Case studies using the model will be conducted to simulate drift and evolution of oil spill events observed in SAR images. The results of the project will be disseminated via scientific publications and by demonstration to users and agencies working with marine monitoring. The project lasts for two years (2007 - 2009) and is funded under INTAS Thematic Call with ESA 2006.

  9. Black patients sustain vision loss while White and South Asian patients gain vision following delamination or segmentation surgery for tractional complications associated with proliferative diabetic retinopathy.

    PubMed

    Mastropasqua, R; Luo, Y H-L; Cheah, Y S; Egan, C; Lewis, J J; da Cruz, L

    2017-10-01

    PurposeThis retrospective comparative case series aims to determine whether patient ethnicity (White versus South Asian versus Black) is related to the outcome of surgical treatment for traction complications of severe proliferative diabetic retinopathy (PDR).SettingMoorfields Eye Hospital London, UK.MethodsAll patients who underwent vitrectomy with, delamination and/or segmentation for PDR over a 5-year period (2009-2014) were reviewed retrospectively. Patients were divided into White, South Asian or Black groups, and their age, gender, HbA1C and type of diabetes were recorded. A total of 484 patients (253 White, 117 South Asian, 114 Black) were included. Twenty-one patients were excluded due to inadequate documentation.OutcomesLogMAR Visual acuity (converted from Snellen) (VA), was recorded pre-operatively and ~6 months post surgery (range 5-8 months). Surgical outcome was classified according to the type and duration of tamponade required post-operatively.ResultsPre-operative VA and HbA1C values were similar across all three ethnic groups (P=0.64 and 0.569, respectively). Change in VA (mean±SD) was 0.41±0.78, 0.14±0.76 and -0.26±0.57 in White, South Asian and Black patient groups respectively (P<0.001). Multiple regression analysis showed that post-op VA was significantly related to race and pre-op VA only (both P<0.001). The Black patient group were more likely to require silicone oil tamponade (P<0.001) and long-term retention of silicone oil (P<0.001) than the White and South Asian patient groups.ConclusionsThis study demonstrates that Black patients on average lose vision following delamination surgery for traction complications of PDR while White and South Asian patients gain vision. The same group is also at higher risk of retaining silicone more than 6 months after surgery. This difference remains even when corrected for glycaemic control. The higher risk of visual loss and long-term retention of silicone oil in black patients requires further investigation. If these results are confirmed, surgeons should consider their patients' ethnicity before proceeding with surgical treatment of diabetic tractional detachment.

  10. Strategic Petroleum Reserve, West Hackberry oil storage cavern fire and spill of September 21, 1978: an environmental assessment. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Taylor, A

    1980-02-29

    This report summarizes an environmental assessment of the fire and oil spill at the Strategic Petroleum Reserve site, West Hackberry, Louisiana. Subjective identification of oil contaminated habitats was supported by a more rigorous classification of samples utilizing discriminant analysis. Fourteen contaminated stations were identified along the shore of Black Lake just north and west of Wellpad 6, encompassing approximately 9 hectares. Seasonal variation in the structures of marsh and lake bottom communities in this contaminated area were not generally distinguishable from that of similar communities in uncontaminated habitats along the southern and southeastern shores of Black Lake. The major impactmore » of spilled oil on the marsh vegetation was to accelerate the natural marsh deterioration which will eventually impact animals dependent on marsh vegetation for habitat structure. Vanadium, the predominate trace metal in the oil, and pyrogenic products due to the fire were found at the most distant sampling site (5 km) from Cavern 6 during Phase I, but were not detected downwind of the fire in excess of background levels in the later phases. Remote sensing evaluation of vegetation under the plume also indicated that stress existed immediately after the fire, but had disappeared by the end of the 1-year survey.« less

  11. Acceptance by black-tailed deer of foliage treated with herbicides.

    Treesearch

    Dan L. Campbell; James Evans; Gerald D. Lindsey; William E. Dusenberry

    1981-01-01

    To test their acceptance of foliage treated with herbicides, captive black-tailed deer were exposed to Douglas-fir seedlings and salal treated with standard formulations of 2,4,5-T, 2,4-D, atrazine, dalapon, fosamine, and glyphosate herbicides. Carriers were diesel oil and water. Tests were made from November 1977 through February 1978. Deer readily browsed 2,4,5-T...

  12. 49 CFR 172.327 - Petroleum sour crude oil in bulk packaging.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... least 100 mm (3.9 inches). The width of the border forming the square-on-point marking must be at least... hydrogen sulfide vapors may occur. (b) The border of the square-on-point must be black or red on a white or other suitable contrasting background. The symbol must be black and located in the center of the square...

  13. A comprehensive study on the phenolic profile of widely used culinary herbs and spices: rosemary, thyme, oregano, cinnamon, cumin and bay.

    PubMed

    Vallverdú-Queralt, Anna; Regueiro, Jorge; Martínez-Huélamo, Miriam; Rinaldi Alvarenga, José Fernando; Leal, Leonel Neto; Lamuela-Raventos, Rosa M

    2014-07-01

    Herbs and spices have long been used to improve the flavour of food without being considered as nutritionally significant ingredients. However, the bioactive phenolic content of these plant-based products is currently attracting interest. In the present work, liquid chromatography coupled to high-resolution/accurate mass measurement LTQ-Orbitrap mass spectrometry was applied for the comprehensive identification of phenolic constituents of six of the most widely used culinary herbs (rosemary, thyme, oregano and bay) and spices (cinnamon and cumin). In this way, up to 52 compounds were identified in these culinary ingredients, some of them, as far as we know, for the first time. In order to establish the phenolic profiles of the different herbs and spices, accurate quantification of the major phenolics was performed by multiple reaction monitoring in a triple quadrupole mass spectrometer. Multivariate statistical treatment of the results allowed the assessment of distinctive features among the studied herbs and spices. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. The effects of aromatherapy on nicotine craving on a U.S. campus: a small comparison study.

    PubMed

    Cordell, Barbara; Buckle, Jane

    2013-08-01

    To evaluate the effect of two inhaled essential oils (black pepper or angelica) on the nicotine habits of students, staff, and faculty on a U.S. college campus. Comparative study with pre-/post-test measures. Community college in rural East Texas. Convenience sample of 20 volunteers from the college community (students, faculty, and staff) who were regular (daily) users of nicotine (cigarettes, snuff, or chewing tobacco). Inhalation of one drop of essential oil on a tissue for 2 minutes when participant was craving nicotine. (1) Pre-inhalation journal recording of self-assessed level of craving for nicotine on a 0-10 scale, (2) post-inhalation journal recording of self-assessed level of craving for nicotine on a 0-10 scale, and (3) minutes that participant waited from start of inhalation until next use of tobacco. Both black pepper and angelica reduced the level of nicotine craving and allowed a longer delay before next use of tobacco. However, black pepper reduced the level of craving more than did angelica, and angelica allowed for a longer delay than did black pepper. Aromatherapy may be useful in nicotine withdrawal. Further studies are warranted.

  15. Effects of Nigella sativa supplementation on blood parameters and anthropometric indices in adults: A systematic review on clinical trials

    PubMed Central

    Mohtashami, Alireza; Entezari, Mohammad Hasan

    2016-01-01

    Background: Nigella sativa (N. sativa) has been used in traditional medicine and several studies have been performed in the last decades to reveal the effects of it on different medical disorders such as diabetes, dyslipidemia, hypertension, and obesity. We evaluated the effects of N. sativa supplementation on lipid profiles, glycemic control, blood pressure (BP), and some anthropometric indices in humans. Materials and Methods: A search on published studies was done by using databases including PubMed, Google Scholar, Thomas Reuters Web of Science, and Cochrane. Medical subject headings (MeSH) terms searched included “N. sativa,” “Black seed,” “Black cumin,” “kalonji,” and “Triglycerides,” “Cholesterol,” “Lipoproteins,” “LDL,” “Lipoproteins,” “HDL,” “Blood glucose,” “Hemoglobin A,” “Glycosylated,” “Blood pressure,” “Body mass index,” “Waist circumference”. Initially 515 articles were extracted. Four hundred ninety-two papers that were unrelated, reviews, animal studies, and combined and duplicated studies were excluded, 23 articles were eligible for this review. Results: After analyzing 23 articles including 1531 participants, these results were achieved: In 4 trials, N. sativa reduced BP, but in 5 trials it could not. Fasting blood sugar (FBS) was reduced significantly in 13 studies. In addition, N. sativa reduced levels of glycosylated hemoglobin (HbA1c). Although weight and waist circumference (WC) in 2 articles were reduced significantly, in 6 articles they were not. Fluctuation in lipid profile in the articles was very controversial, being significant in many of them but not in others. Conclusion: Our systematic review revealed that N. sativa supplementation might be effective in glycemic control in humans. PMID:27904549

  16. Severe Storm in the Sea of Azov

    NASA Technical Reports Server (NTRS)

    2007-01-01

    A fierce storm struck both the Black Sea and the Sea of Azov on November 11, 2007. According to news reports, as many as 10 ships either sank or ran aground, one of them an oil tanker. The Russian tanker Volganeft-139 was anchored to the sea floor in the Kerch Strait linking the Black and Azov Seas when 108-kilometer- (67-mile-) per-hour winds tore the ship apart. As of November 12, up to 2,000 metric tons of fuel oil had leaked from the ship. On November 11, 2007, the day the storm struck, the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Aqua satellite captured this image of the region. Thick clouds obscure the view of much of the land area, including Ukraine, Belarus, western Russia, and Georgia. Clouds also obscure the Sea of Azov, although skies over the Black Sea are somewhat clearer. Over the Sea of Azov and immediately to the north, the clouds form a vague swirling pattern, suggestive of a low-pressure cyclonic system.

  17. 77 FR 7637 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-13

    ... Trust 1 and United States Oil Fund at $0.50 Strike Price Intervals Where the Strike Price Is Less Than... interested persons. \\1\\ ``iShares[supreg]'' is a registered trademark of BlackRock Institutional Trust...[supreg] Silver Trust \\5\\ and United States Oil Fund at $0.50 strike price intervals where the strike...

  18. 77 FR 7634 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-13

    ...] Silver Trust \\1\\ and United States Oil Fund at $0.50 Strike Price Intervals Where the Strike Price Is Less Than $75 \\1\\ ``iShares[supreg]'' is a registered trademark BlackRock Institutional Trust Company...] Silver Trust \\5\\ and United States Oil Fund at $0.50 strike price intervals where the strike price is...

  19. Domestic airborne black carbon and exhaled nitric oxide in children in NYC

    PubMed Central

    Cornell, Alexandra G.; Chillrud, Steven N.; Mellins, Robert B.; Acosta, Luis M.; Miller, Rachel L.; Quinn, James W.; Yan, Beizhan; Divjan, Adnan; Olmedo, Omar E.; Lopez-Pintado, Sara; Kinney, Patrick L.; Perera, Frederica P.; Jacobson, Judith S.; Goldstein, Inge F.; Rundle, Andrew G.; Perzanowski, Matthew S.

    2012-01-01

    Differential exposure to combustion by-products and allergens may partially explain the marked disparity in asthma prevalence (3%–18%) among New York City neighborhoods. Subclinical changes in airway inflammation can be measured by fractional exhaled nitric oxide (FeNO). FeNO could be used to test independent effects of these environmental exposures on airway inflammation. Seven and eight year-old children from neighborhoods with lower (range 3–9%, n=119) and higher (range 11–18%, n=121) asthma prevalence participated in an asthma case-control study. During home visits, FeNO was measured, and samples of bed dust (allergens) and air (black carbon) were collected. Neighborhood built-environment characteristics were assessed for the 500m surrounding participants’ homes. Airborne black carbon concentrations in homes correlated with neighborhood asthma prevalence (P<0.001) and neighborhood densities of truck routes (P<0.001) and buildings burning residual oil (P<0.001). FeNO concentrations were higher among asthmatics with compared to asthmatics without frequent wheeze (≥4 times/year) (P=0.002). FeNO concentrations correlated with domestic black carbon among children without seroatopy (P=0.012) and with dust mite allergen among children with seroatopy (P=0.020). The association between airborne black carbon in homes and both neighborhood asthma prevalence and FeNO suggest that further public health interventions on truck emissions standards and residual oil use are warranted. PMID:22377682

  20. Uranium in the Upper Cambrian black shale of Sweden

    USGS Publications Warehouse

    McKelvey, Vincent Ellis

    1955-01-01

    The Peltura zone of the Upper Cambrian black shales of Sweden contains about 0.02 percent uranium. Maximum amounts are present in rocks deposited in an embayment in the sea and in rocks in or closely adjacent to that part of the vertical sequence that contains maximum amounts of distillable oil, total organic matter, pyrite, and a black highly uraniferous kerogen called "kolm". Available data suggest that the precipitation of uranium is favored by a low redox potential and that the uranium in the shale matrix may be in fine-grained kolm.

  1. The Bone Black Pigment Identification by Noninvasive, In Situ Infrared Reflection Spectroscopy

    PubMed Central

    Malagodi, Marco; Vagnini, Manuela

    2018-01-01

    Two real case studies, an oil painting on woven paper and a cycle of mural paintings, have been presented to validate the use of infrared reflection spectroscopy as suitable technique for the identification of bone black pigment. By the use of the sharp weak band at 2013 cm−1, it has been possible to distinguish animal carbon-based blacks by a noninvasive method. Finally, an attempt for an eventual assignment for the widely used sharp band at 2013 cm−1 is discussed. PMID:29736290

  2. Zooplankton fecal pellets link fossil fuel and phosphate deposits

    USGS Publications Warehouse

    Porter, K.G.; Robbins, E.I.

    1981-01-01

    Fossil zooplankton fecal pellets found in thinly bedded marine and lacustrine black shales associated with phosphate, oil, and coal deposits, link the deposition of organic matter and biologically associated minerals with planktonic ecosystems. The black shales were probably formed in the anoxic basins of coastal marine waters, inland seas, and rift valley lakes where high productivity was supported by runoff, upwelling, and outwelling. Copyright ?? 1981 AAAS.

  3. Investigation of an Error Theory for Conjoint Measurement Methodology.

    DTIC Science & Technology

    1983-05-01

    1ybren, 1982; Srinivasan and Shocker, 1973a, 1973b; Ullrich =d Cumins , 1973; Takane, Young, and de Leeui, 190C; Yount,, 1972’. & OEM...procedures as a diagnostic tool. Specifically, they used the oompted STRESS - value and a measure of fit they called PRECAP that could be obtained

  4. Estrogen Receptor Mutants/Variants in Human Breast Cancer.

    DTIC Science & Technology

    1997-12-01

    a recipient of a USAMRMC Postdoctoral Fellowship Award. The authors thank Caroline Cumins -Leygue and Helen Bergen for labora- tory assistance with...of their ER status as determined by ligand binding assay. It should be stressed that an additional band can be observed in most of the samples. This

  5. Authentication of commercial spices based on the similarities between gas chromatographic fingerprints.

    PubMed

    Matsushita, Takaya; Zhao, Jing Jing; Igura, Noriyuki; Shimoda, Mitsuya

    2018-06-01

    A simple and solvent-free method was developed for the authentication of commercial spices. The similarities between gas chromatographic fingerprints were measured using similarity indices and multivariate data analyses, as morphological differentiation between dried powders and small spice particles was challenging. The volatile compounds present in 11 spices (i.e. allspice, anise, black pepper, caraway, clove, coriander, cumin, dill, fennel, star anise, and white pepper) were extracted by headspace solid-phase microextraction, and analysed by gas chromatography-mass spectrometry. The largest 10 peaks were selected from each total ion chromatogram, and a total of 65 volatiles were tentatively identified. The similarity indices (i.e. the congruence coefficients) were calculated using the data matrices of the identified compound relative peak areas to differentiate between two sets of fingerprints. Where pairs of similar fingerprints produced high congruence coefficients (>0.80), distinctive volatile markers were employed to distinguish between these samples. In addition, hierarchical cluster analysis and principal component analysis were performed to visualise the similarity among fingerprints, and the analysed spices were grouped and characterised according to their distinctive major components. This method is suitable for screening unknown spices, and can therefore be employed to evaluate the quality and authenticity of various spices. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  6. Physico-chemical characteristics and free fatty acid composition of dry fermented mutton sausages as affected by the use of various combinations of starter cultures and spices.

    PubMed

    Zhao, Lihua; Jin, Ye; Ma, Changwei; Song, Huanlu; Li, Hui; Wang, Zhenyu; Xiao, Shan

    2011-08-01

    The microbiological, physico-chemical and free fatty acid composition of dry fermented mutton sausages were determined during ripening and storage. Three sausage mixtures (starter culture [SC], SC and black pepper [SC+BP] and SC, BP and cumin [SC+BP+C]) were compared with a control (CO). In general, the lactic acid bacteria populations in the SC+BP increased significantly to 9 log CFU/g and were higher than the CO (8 log CFU/g) (P<0.05) from fermentation to ripening. The pH values of the SC, SC+BP and SC+BP+C were 4.81, 4.55 and 4.53 respectively, significantly lower (P<0.05) than the CO at the end of fermentation. The water activity (a(w)) in all sausages decreased significantly to 0.88 at Day 7. The total free fatty acid (TFFA) in the treatments increased significantly (P<0.05) during ripening and storage. The levels of MUFA+PUFA/SFA in SC+BP and SC+BP+C at Day 7 were 2.44 and 2.31 respectively, higher than the control (1.65) (P>0.05). Copyright © 2011 Elsevier Ltd. All rights reserved.

  7. Crew Earth Observations (CEO) taken during Expedition 8

    NASA Image and Video Library

    2004-03-24

    ISS008-E-18935 (24 March 2004) --- This photo of a large black smoke plume from an oil fire was taken by an Expedition 8 crewmember on the International Space Station as the Station orbited northeastward across Saudi Arabia. According to a front-page story in the New York Times, an oil pipeline near the channel of Shatt al Arab in southeastern Iraq on the Persian Gulf ruptured the day before. The resulting oil spill ignited and its smoke can here be seen to extend over 100 miles northwestward into southern Iraq.

  8. Poached Salmon

    MedlinePlus

    ... page: https://medlineplus.gov/recipe/poachedsalmon.html Poached Salmon To use the sharing features on this page, ... olive oil Ground black pepper, to taste For salmon: 4 salmon steaks, 5 oz each 3 cups ...

  9. Development of Antimicrobial Biocomposite Films to Preserve the Quality of Bread.

    PubMed

    Figueroa-Lopez, Kelly J; Andrade-Mahecha, Margarita María; Torres-Vargas, Olga Lucía

    2018-01-19

    This study focused on the development of gelatin-based films with incorporation of microcrystalline cellulose as reinforcement material. Clove ( Syzygium aromaticum ), nutmeg ( Myristica fragrans ), and black pepper ( Piper nigrum ) oleoresins containing antimicrobial compounds of natural origin were incorporated into the films. The mechanical, thermal, optical, and structural properties, as well as color, seal strength and permeability to water vapor, light, and oil of the films were determined. Adding oleoresins to the gelatin matrix increased the elongation of the material and significantly diminished its permeability to water vapor and oil. Evaluation of the potential use of films containing different oleoresins as bread packaging material was influenced by the film properties. The biocomposite film containing oleoresin from black pepper was the most effective packaging material for maintaining bread's quality characteristics.

  10. A Study of the Fluid-Dynamic Pressure Fields on Compressor Reed Valves.

    DTIC Science & Technology

    1985-12-01

    AIR FORCE INSTITUTE OF TECHNOLOGY ": Wright-Patterson Air Force Base, Ohio . . .. " . _ .01 1...mixture containing white pigment, the test rig was painted black to insure better contrast for photography. The oil was dotted on the base plate of...test rig black roughened the Eurtace and slightly reduced the sharpness ox the shock line. For con.iiuration ., three test pressures were chosen. Fhe

  11. Irradiation of organic matter by uranium decay in the Alum Shale, Sweden

    NASA Astrophysics Data System (ADS)

    Lewan, M. D.; Buchardt, B.

    1989-06-01

    The Alum Shale of Sweden contains black shales with anomalously high uranium concentrations in excess of 100 ppm. Syngenetic or early diagenetic origin of this uranium indicates that organic matter within these shales has been irradiated by decaying uranium for approximately 500 Ma. Radiation-induced polymerization of alkanes through a free-radical cross-linking mechanism appears to be responsible for major alterations within the irradiated organic matter. Specific radiation-induced alterations include generation of condensate-like oils at reduced yields from hydrous pyrolysis experiments, decrease in atomic H/C ratios of kerogens, decrease in bitumen/organic-carbon ratios, and a relative increase in low-molecular weight triaromatic steroid hydrocarbons. Conversely, stable carbon isotopes of kerogens, reflectance of vitrinite-like macerais, oil-generation kinetics, and isomerization of 20R to 20S αα C 29-steranes were not affected by radiation. The radiation dosage needed to cause the alterations observed in the Alum Shale has been estimated to be in excess of 10 5 Mrads with respect to organic carbon. This value is used to estimate the potential for radiation damage to thermally immature organic matter in black shales through the geological rock record. High potential for radiation damage is not likely in Cenozoic and Mesozoic black shales but becomes more likely in lower Paleozoic and Precambrian black shales.

  12. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Andres, B.A.

    Black oystercatchers (Haematopus bachmani) were studied in central Prince William Sound, Alaska, to determine the effects that the Exxon Valdez oil spill had on their population, reproductive biology, and behavioral ecology. Estimates of direct mortality of black oystercatchers varied widely among the methods used to assess losses and ranged from 4% to 57% of the population inhabitating the spill zone; mortality probably did not exceed 20% of the population. Although the black oystercatcher population appears to be recovering in some areas of the Sound, delayed reproduction in the species precludes determination of the total effect of the spill on themore » population until at least 1994.« less

  13. Black Lipid Membranes at Bifaces

    PubMed Central

    Ti Tien, H.

    1968-01-01

    Black lipid membranes (BLM) less than 90 A thick have been shown to be the most realistic approach to biological membrane models. This paper describes the formation characteristics, optical properties, and thermodynamics of BLM at water/oil/water bifaces. In particular, the nature of the Plateau-Gibbs border which supports the black membrane is analyzed in some detail. The formation of BLM at the biface involves a spontaneous reduction of the free energy of the system. As long as the integrity of the membrane is maintained, the limiting structure of the BLM represents the lowest free energy configuration. PMID:19873618

  14. Fertilization and Colors of Plastic Mulch Affect Biomass and Essential Oil of Sweet-Scented Geranium

    PubMed Central

    Silva, Anderson de Carvalho; dos Santos, Wallace Melo; Prata, Paloma Santana; Alves, Péricles Barreto

    2014-01-01

    Sweet-scented geranium (Pelargonium graveolens L'Hér), a plant belonging to the Geraniaceae family, has medicinal and aromatic properties and is widely used in the cosmetic, soap, perfume, aromatherapy, and food industries. The aim of this study was to evaluate the influence of fertilization and the use of different colors of plastic mulch on sweet-scented geranium biomass and essential oil. Three colors of plastic mulch (black, white, and silver-colored) and a control without plastic mulch were assessed along with three fertilizers (20,000 L·ha−1 of cattle manure; 1,000 kg·ha−1 of NPK 3-12-6; and 20,000 L·ha−1 of cattle manure + 1,000 kg·ha−1 of NPK 3-12-6 fertilizer) and a control without fertilizer. The absence of a soil cover negatively influenced the agronomical variables, while coverage with plastic mulch was associated with increased biomass. The use of fertilizer had no effect on the evaluated agronomic variables. When cattle manure and NPK 3-12-6 were used together, combined with white or black plastic mulch, the highest yields of essential oil were obtained. For the silver-colored plastic mulch, higher amounts of essential oil (6,9-guaiadien) were obtained with mineral fertilizer. PMID:24757440

  15. Prevention of Post-Radiotherapy Failure in Prostate Cancer by Vitamin D

    DTIC Science & Technology

    2005-03-01

    cur- 55. Mitchell MF, Hittelman WN, Hong WK et al. The natural history cumin , a chemo preventive agent, in patients with high-risk or of cervical...chromosomal DNA loops during oxidative stress . Genes & Dev 13:1553-1560. 16 1999 Chen AY, Choy H and Rothenberg ML. DNA topoisomerase I-targeting drugs as

  16. Symbolic Architectures for Cognition

    DTIC Science & Technology

    1989-01-01

    Perceptual and mnotor interfaces b. Buffering and interrupts c. Real-time demands for action c. Continuous acquis;ition of knowledge We stress that...memorie- Workin.g menmki ’cv I REGULAR I LINE LONG JS18 17265SSSS6 U3 0411- 09583 Pa 1 1 DEK Coportio 11 Cumins Prk Wo burn, MA 01801 (617)935-8070

  17. Design and Integration of a Scent Delivery System in the Computer Assisted Rehabilitation Environment (CAREN)

    DTIC Science & Technology

    2013-12-06

    Sulfurous Volcano 1643 Burnt Flesh 219 Burning Rubber 1645 Dead Body 243 Diesel Exhaust 1690 Vomit 252 Oily Machinery/Hydraulic Fluid Food 1432...Vehicle 1664 Cumin 1650 Tar Asphalt 1680 Rosemary Focaccia Bread 1680 Car Bomb 1990 Garlic 1905 Turpentine 1992 Mesquite BBQ Scent System

  18. Aflatoxins in spices marketed in Portugal.

    PubMed

    Martins, M L; Martins, H M; Bernardo, F

    2001-04-01

    Seventy-nine prepackaged samples of 12 different types of spice powders (five cardamom, five cayenne pepper, eight chilli, five cloves, seven cumin, five curry) powder, five ginger, five mustard, 10 nutmeg, 12 paprika, five saffron and seven white pepper) were selected from supermarkets and ethnic shops in Lisbon (Portugal) for estimation of aflatoxins by immunoaffinity column clean-up followed by HPLC. Aflatoxin B1 (AFB1) was detected in 34 samples of prepackaged spices (43.0%). All of the cayenne pepper samples were contaminated with levels ranging from 2 to 32 microg AFB1/kg. Three nutmeg samples contained levels ranging from 1 to 5 microg/kg, three samples had levels ranging from 6 to 20 microg/kg, and there were two with 54 microg/kg and 58 microg/ kg. Paprika contained levels of aflatoxin B1 ranging from 1 to 20 microg/kg. Chilli, cumin, curry powder, saffron and white pepper samples had levels ranging from 1 to 5 microg/kg. Aflotoxins were not detected in cardamon, cloves, ginger and mustard. None of the samples analysed contained aflatoxins B2, G1 and G2.

  19. Effective Recovery of Vanadium from Oil Refinery Waste into Vanadium-Based Metal-Organic Frameworks.

    PubMed

    Zhan, Guowu; Ng, Wei Cheng; Lin, Wenlin Yvonne; Koh, Shin Nuo; Wang, Chi-Hwa

    2018-03-06

    Carbon black waste, an oil refinery waste, contains a high concentration of vanadium(V) leftover from the processing of crude oil. For the sake of environmental sustainability, it is therefore of interest to recover the vanadium as useful products instead of disposing of it. In this work, V was recovered in the form of vanadium-based metal-organic frameworks (V-MOFs) via a novel pathway by using the leaching solution of carbon black waste instead of commercially available vanadium chemicals. Two different types of V-MOFs with high levels of crystallinity and phase purity were fabricated in very high yields (>98%) based on a coordination modulation method. The V-MOFs exhibited well-defined and controlled shapes such as nanofibers (length: > 10 μm) and nanorods (length: ∼270 nm). Furthermore, the V-MOFs showed high catalytic activities for the oxidation of benzyl alcohol to benzaldehyde, indicating the strong potential of the waste-derived V-MOFs in catalysis applications. Overall, our work offers a green synthesis pathway for the preparation of V-MOFs by using heavy metals of industrial waste as the metal source.

  20. Development and test of video systems for airborne surveillance of oil spills

    NASA Technical Reports Server (NTRS)

    Millard, J. P.; Arvesen, J. C.; Lewis, P. L.

    1975-01-01

    Five video systems - potentially useful for airborne surveillance of oil spills - were developed, flight tested, and evaluated. The systems are: (1) conventional black and white TV, (2) conventional TV with false color, (3) differential TV, (4) prototype Lunar Surface TV, and (5) field sequential TV. Wavelength and polarization filtering were utilized in all systems. Greatly enhanced detection of oil spills, relative to that possible with the unaided eye, was achieved. The most practical video system is a conventional TV camera with silicon-diode-array image tube, filtered with a Corning 7-54 filter and a polarizer oriented with its principal axis in the horizontal direction. Best contrast between oil and water was achieved when winds and sea states were low. The minimum detectable oil film thickness was about 0.1 micrometer.

  1. Antimicrobial activity of olive oil, vinegar, and various beverages against foodborne pathogens.

    PubMed

    Medina, Eduardo; Romero, Concepción; Brenes, Manuel; De Castro, Antonio

    2007-05-01

    The survival of foodborne pathogens in aqueous extracts of olive oil, virgin olive oil, vinegar, and several beverages was evaluated. Vinegar and aqueous extracts of virgin olive oil showed the strongest bactericidal activity against all strains tested. Red and white wines also killed most strains after 5 min of contact, black and green tea extracts showed weak antimicrobial activity under these conditions, and no effect was observed for the remaining beverages (fruit juices, Coca-Cola, dairy products, coffee, and beer). The phenolic compound content of the aqueous olive oil and virgin olive oil extracts could explain their antibacterial activity, which was also confirmed in mayonnaises and salads used as food models. Virgin olive oil in mayonnaises and salads reduced the counts of inoculated Salmonella Enteritidis and Listeria monocytogenes by approximately 3 log CFU/g. Therefore, olive oil could be a hurdle component in certain processed foods and exert a protective effect against foodborne pathogens when contaminated foods are ingested.

  2. Effect of artificial regulations of Artemia n-3 HUFA content on growth and survival of black seabream ( Sparus macrocephalus) larvae

    NASA Astrophysics Data System (ADS)

    Liu, Jing-Ke

    1998-06-01

    The requirement for dietary n-3 HUFA (highly unsaturated fatty acid) for growth and survival of black seabream ( Sparus macrocephalus) larvae was tested using Artemia with various levels of n-3 HUFA. Four treatments with Artemia differing in their n-3 HUFA were prepared by feeding them various oil emulsions. The results indicated that dietary n-3 HUFA significantly influences fish n-3 HUFA levels and are necessary for good growth and survival of black seabream larvae. The proper value of n-3 HUFA level and DHA, EPA level in Artemia should be 4.273% and 0.873% (wet weight) for good growth and survival in black seabream larvae respectively.

  3. Isolation, characterization and identification of pericarp-degrading bacteria for the production of off-odour-free white pepper from fresh berries of Piper nigrum L.

    PubMed

    Vinod, V; Kumar, A; Zachariah, T J

    2014-04-01

    To isolate, fermentatively evaluate and identify black pepper (Piper nigrum L.)-associated bacteria for the microbial decortication of fresh ripened berries and dried black pepper for preparation of off-odour-free white pepper. Among 45 bacterial isolates obtained from black pepper, seven of them were found to decorticate black pepper (>60%) and fresh pepper berries (98-100%) into white pepper within 5 days of immersion in bacterial suspension. The 16S rRNA genes (1500-bp amplicon) of these bacteria were sequenced, and species identity was established by closest match in GenBank. Superior-quality white pepper was obtained with Bacillus subtilis (IISR WP 33, 34, 38), Bacillus licheniformis (IISR WP 43), Acinetobacter baumanii (IISR WP 35), Klebsiella pneumoniae (IISR WP 19) and Microbacterium barkeri (IISR WP25). The bacterial isolates were found to secrete multiple hydrolytic enzymes such as cellulase, pectinase, amylase, protease and xylanase. Bacterial cultures were deposited with International Depository Authority at Microbial Type Culture Collection, India, as patent deposits as prescribed in Budapest Treaty for microbial deposits. The white pepper, thus obtained from bacterial decortication process, was free from off-odour compound, especially skatole. Other biochemical constituents such as oleoresin, piperine and essential oils were found in the acceptable range. The bacterial decortication did not affect inherent constituents of pepper such as essential oil constituents, oleoresin and piperine content. One of the most significant findings of the work is identification of specific bacterial species for decortication of fresh berries or black pepper berries into value-added white pepper. This work paved way for developing a technological process for microbial decortication of fresh/black pepper for the production of superior-quality white pepper. © 2014 The Society for Applied Microbiology.

  4. Antifungal activity and computational study of constituents from Piper divaricatum essential oil against Fusarium infection in black pepper.

    PubMed

    da Silva, Joyce Kelly R; Silva, José Rogério A; Nascimento, Soelange B; da Luz, Shirlley F M; Meireles, Erisléia N; Alves, Cláudio N; Ramos, Alessandra R; Maia, José Guilherme S

    2014-11-04

    Fusarium disease causes considerable losses in the cultivation of Piper nigrum, the black pepper used in the culinary world. Brazil was the largest producer of black pepper, but in recent years has lost this hegemony, with a significant reduction in its production, due to the ravages produced by the Fusarium solani f. sp. piperis, the fungus which causes this disease. Scientific research seeks new alternatives for the control and the existence of other Piper species in the Brazilian Amazon, resistant to disease, are being considered in this context. The main constituents of the oil of Piper divaricatum are methyleugenol (75.0%) and eugenol (10.0%). The oil and these two main constituents were tested individually at concentrations of 0.25 to 2.5 mg/mL against F. solani f. sp. piperis, exhibiting strong antifungal index, from 18.0% to 100.0%. The 3D structure of the β-glucosidase from Fusarium solani f. sp. piperis, obtained by homology modeling, was used for molecular docking and molecular electrostatic potential calculations in order to determine the binding energy of the natural substrates glucose, methyleugenol and eugenol. The results showed that β-glucosidase (Asp45, Arg113, Lys146, Tyr193, Asp225, Trp226 and Leu99) residues play an important role in the interactions that occur between the protein-substrate and the engenol and methyleugenol inhibitors, justifying the antifungal action of these two phenylpropenes against Fusarium solani f. sp. piperis.

  5. Cholinesterase and glutathione S-transferase activities of three mollusc species from the NW Portuguese coast in relation to the 'Prestige' oil spill.

    PubMed

    Tim-Tim, Ana L S; Morgado, Fernando; Moreira, Susana; Rangel, Rui; Nogueira, António J A; Soares, Amadeu M V M; Guilhermino, Lúcia

    2009-12-01

    In November 2002, the tanker 'Prestige' released about 19,000 tonnes of a heavy fuel oil (no. 6) before sinking with about 58,000 tonnes of its cargo, 135 miles from Cabo Finisterra (Spain). A considerable part of the released fuel oil reached the Galician coast, causing a heavy black tide and an ecological disaster. Although the black tide did not reach the NW coast of Portugal, it is possible that some of the fuel oil or its components also arrived to this area directly through the sea water and/or indirectly through the food chain. Therefore, the aim of this study was to investigate possible changes in two widely used biomarkers, the activity of the enzymes cholinesterases (ChE) and glutathione S-transferases (GST), of three molluscs (Mytilus galloprovincialis, Nucella lapillus and Monodonta lineata) from wild populations of the NW Portuguese coast in relation to the 'Prestige' oil spill. Molluscs were collected seasonally before (autumn 2002) and after (winter 2002/2003), spring and summer 2003) the oil spill at several sites along the Portuguese NW coast. Enzymatic activities determined before the accident were compared with those determined at different times after the oil spill taking into consideration abiotic factors. Information from different parameters was integrated by Redundancy Analysis and Principal Response Curves (PRC). Results show that GST and ChE activities were influenced by abiotic factors. Despite this influence, the results of PRC analysis also suggest that some of the fuel oil reached the NW Portuguese coast changing the patterns of ChE and GST activities of local populations of rocky shore species. Furthermore, the present study highlights the need of long-term monitoring with wild populations to assess both historical and punctual effects of pollution in the marine environment.

  6. Analyzing the Rationales Behind Russia’s Intervention in Ukraine

    DTIC Science & Technology

    2016-03-01

    87  Figure 4.  Major Natural Gas Supply Pipelines from Russia to Europe ....................91  Figure 5.  Gas Price Disparities between...Transporting natural gas is more difficult than transporting oil. While oil can be moved via pipeline , ship, train, or truck, natural gas must either be...Blue Stream pipeline through the Black Sea carry Russian gas directly to Germany and Turkey, respectively. In contrast, the Yamal pipeline traverses

  7. Propagation of uncertainties through the oil spill model MEDSLIK-II: operational application to the Black Sea

    NASA Astrophysics Data System (ADS)

    Liubartseva, Svitlana; Coppini, Giovanni; Ciliberti, Stefania Angela; Lecci, Rita

    2017-04-01

    In operational oil spill modeling, MEDSLIK-II (De Dominicis et al., 2013) focuses on the reliability of the oil drift and fate predictions routinely fed by operational oceanographic and atmospheric forecasting chain. Uncertainty calculations enhance oil spill forecast efficiency, supplying probability maps to quantify the propagation of various uncertainties. Recently, we have developed the methodology that allows users to evaluate the variability of oil drift forecast caused by uncertain data on the initial oil spill conditions (Liubartseva et al., 2016). One of the key methodological aspects is a reasonable choice of a way of parameter perturbation. In case of starting oil spill location and time, these scalars might be treated as independent random parameters. If we want to perturb the underlying ocean currents and wind, we have to deal with deterministic vector parameters. To a first approximation, we suggest rolling forecasts as a set of perturbed ocean currents and wind. This approach does not need any extra hydrodynamic calculations, and it is quick enough to be performed in web-based applications. The capabilities of the proposed methodology are explored using the Black Sea Forecasting System (BSFS) recently implemented by Ciliberti et al. (2016) for the Copernicus Marine Environment Monitoring Service (http://marine.copernicus.eu/services-portfolio/access-to-products). BSFS horizontal resolution is 1/36° in zonal and 1/27° in meridional direction (ca. 3 km). Vertical domain discretization is represented by 31 unevenly spaced vertical levels. Atmospheric wind data are provided by the European Centre for Medium-Range Weather Forecasts (ECMWF) forecasts, at 1/8° (ca. 12.5 km) horizontal and 6-hour temporal resolution. A great variety of probability patterns controlled by different underlying flows is represented including the cyclonic Rim Current, flow bifurcations in anticyclonic eddies (e.g., Sevastopol and Batumi), northwestern shelf circulation, etc. Uncertainty imprints in the oil mass balance components are also analyzed. This work is conducted in the framework of the REACT Project funded by Fondazione CON IL SUD/Brains2South. References Ciliberti, S.A., Peneva, E., Storto, A., Kandilarov, R., Lecci, R., Yang, C., Coppini, G., Masina, S., Pinardi, N., 2016. Implementation of Black Sea numerical model based on NEMO and 3DVAR data assimilation scheme for operational forecasting, Geophys. Res. Abs., 18, EGU2016-16222. De Dominicis, M., Pinardi, N., Zodiatis, G., Lardner, R., 2013. MEDSLIK-II, a Lagrangian marine surface oil spill model for short term forecasting-Part 1: Theory, Geosci. Model Dev., 6, 1851-1869. Liubartseva, S., Coppini, G., Pinardi, N., De Dominicis, M., Lecci, R., Turrisi, G., Cretì, S., Martinelli, S., Agostini, P., Marra, P., Palermo, F., 2016. Decision support system for emergency management of oil spill accidents in the Mediterranean Sea, Nat. Hazards Earth Syst. Sci., 16, 2009-2020.

  8. Fenton process combined with coagulation for the treatment of black liquor from bioethanol wastewater

    NASA Astrophysics Data System (ADS)

    Muryanto, Muryanto; Hanifah, Ummu; Amriani, Feni; Ibadurrahman, Ahmad Faiz; Sari, Ajeng Arum

    2017-11-01

    High amounts of black liquor are generated from bioethanol production by using oil palm empty fruit bunches. The black liquor is waste from alkaline pretreatment, it contains high amount of an alkaline solution (NaOH). The black liquor wastewater was highly contaminated with organic materials, and quite toxic for aquatic ecosystems if discharged directly into waters. This study aimed to determine ability of Fenton process combined with coagulation to treat black liquor. The addition 5% of polyaluminium chloride (PAC) could decolorized black liquor, degraded lignin, and produced sludge 70.64%, 68.28%, and 2.76 gram, respectively. Decolorization of black liquor was in line with degradation of black liquor because lignin is the main compound in black liquor. SEM images after addition of PAC of 5% indicated fragmentation of structure. Fenton reagent consist of 0.7 M FeSO4+ 3M H2O2 has able to decolorize black liquor, degrade lignin, and produce sludge 51.67% and 25.44%, and 0.44 gram, respectively. It was concluded that black liquor wastewater from bioethanol can be treated by using Fenton process combined with coagulation. However, these methods still need improvement to obtain the higher degradation rate, and coagulation sludge needs further consideration.

  9. 78 FR 5423 - Procurement List; Additions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-25

    ... following products are added to the Procurement List: Products NSN: 8950-01-E61-0660--Spice, Oregano, Ground, 6/12 oz. Containers. NSN: 8950-01-E62-0111--Spice, Cumin, Ground, 6/16 oz. Containers. NSN: 8950-01-E60-9321--Spice, Pepper, White, Ground, 6/18 oz. Containers. NSN: 8950-01-E62-0148--Spice, Bay Leaf...

  10. 77 FR 70737 - Procurement List; Proposed Additions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-27

    ... Spices NSN: 8950-01-E60-9321--Spice, Pepper, White, Ground, 6/18 oz. Containers. NSN: 8950-01-E61-0660--Spice, Oregano, Ground, 6/12 oz. Containers. NSN: 8950-01-E62-0111--Spice, Cumin, Ground, 6/16 oz. Containers. NSN: 8950-01-E62-0148--Spice, Bay Leaf, Whole, \\3/8\\ oz. Containers. NPA: CDS Monarch, Webster...

  11. Photoluminescence Studies on InAs/InSb Nanostructures Grown by MBE

    DTIC Science & Technology

    2000-06-23

    temperatures [61. One should stress especially that the main problem here is intermixing of group V elements at the interfaces. Since we used conventional solid...Nicolas, N. J. Mason and B. Zhang, Appl. Phys. Let. 74, 2041 (1999). [5] N. Bertru, A. Baranov, Y. Cuminal , G. Almuneau, F. Genty, A. Joullie, 0. Brandt, A

  12. Compact, High Power, Multi-Spectral Mid-Infrared Semiconductor Laser Package

    DTIC Science & Technology

    2001-10-01

    depositions to map out effects due to stress in the films. Stress in the film will result in the substrate curvature. This curvature results in the...Lasers at 4.5 µm”, IEEE Photon. Technol. Lett., vol. 9, pp. 1573-1575, (1997). 58 18. A. N. Baranov, N. Bertru, Y. Cuminal , G. Boissier, C. Alibert, and

  13. Commander’s Handbook on Military and Civilian Law. Revision

    DTIC Science & Technology

    1992-01-01

    Cuminal Law Divio 23-8 Commander’s Handbook The accused’s possession must be knowing and conscious. Therefore, if the accused didn’t know he or she...of violence and neglect in families, for example, experiencing or witnessing abuse as a child or the stress a family experiences due to the member’s

  14. Decolorization of black liquor from bioethanol G2 production using iron oxide coating sands

    NASA Astrophysics Data System (ADS)

    Barlianti, Vera; Triwahyuni, Eka; Waluyo, Joko; Sari, Ajeng Arum

    2017-01-01

    Bioethanol G2 production using oil palm empty fruit bunch as raw material consists of four steps, namely pretreatment, hydrolysis, fermentation, and purification process. Pretreatment process generates black liquor that causes serious environmental pollution if it is released to the environment. The objective of this research is studying the ability of iron oxide coating sands to adsorb the color of black liquor. The iron oxide coating sands were synthesized from FeCl3.6H2O with quartz sands as support material. This research was conducted on batch mode using black liquor in various pH values. Result obtained that kind of iron oxide on quartz sands's surface was goethite. The result also indicated decreasing of color intensity of black liquor after adsorption process. This research supports local material utilization in environmental technology development to solve some environmental problems.

  15. How often do condoms fail? : A cross-sectional study exploring incomplete use of condoms, condom failures, and other condom problems among Black and White MSM in the Southern U.S

    PubMed Central

    Hernández-Romieu, Alfonso C.; Siegler, Aaron; Sullivan, Patrick S.; Crosby, Richard; Rosenberg, Eli S.

    2015-01-01

    Objectives Compare the occurrence of risk-inducing condom events (condom failures and incomplete use) and the frequency of their antecedents (condom errors, fit/feel problems, and erection problems) between Black and White MSM, and determine the associations between risk-inducing condom events and their antecedents. Methods We studied cross-sectional data of 475 MSM who indicated using a condom as an insertive partner in the previous 6 months enrolled in a cohort study in Atlanta, GA. Results Nearly 40% of Black MSM reported breakage or incomplete use, and they were more likely to report breakage, early removal, and delayed application of a condom than White MSM. Only 31% and 54% of MSM reported correct condom use and suboptimal fit/feel of a condom respectively. The use of oil-based lubricants and suboptimal fit/feel were associated with higher odds of reporting breakage (P = 0.009). Suboptimal fit/feel was also associated with higher odds of incomplete use of condoms (P <0.0001). Conclusions Incomplete use of condoms and condom failures were especially common among Black MSM. Our findings indicate that condoms likely offered them less protection against HIV/STI when compared to White MSM. More interventions are needed, particularly addressing the use of oil-based lubricants and suboptimal fit/feel of condoms. PMID:25080511

  16. Advancing strategic environmental assessment in the offshore oil and gas sector: Lessons from Norway, Canada, and the United Kingdom

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fidler, Courtney, E-mail: crfidler@gmail.com; Noble, Bram, E-mail: b.noble@usask.ca

    2012-04-15

    Abstract: Strategic environmental assessment (SEA) for offshore oil and gas planning and development is utilized in select international jurisdictions, but the sector has received limited attention in the SEA literature. While the potential benefits of and rationale for SEA are well argued, there have been few empirical studies of SEA processes for the offshore sector. Hence, little is known about the efficacy of SEA offshore, in particular its influence on planning and development decisions. This paper examines SEA practice and influence in three international offshore systems: Norway, Atlantic Canada and the United Kingdom, with the intent to identify the challenges,more » lessons and opportunities for advancing SEA in offshore planning and impact assessment. Results demonstrate that SEA can help inform and improve the efficacy and efficiency of project-based assessment in the offshore sector, however weak coordination between higher and lower tiers limit SEA's ability to influence planning and development decisions in a broad regional environmental and socioeconomic context. - Highlights: Black-Right-Pointing-Pointer SEA can inform and improve the efficacy and efficiency of project EA offshore Black-Right-Pointing-Pointer Scope and deliverables of SEA offshore often differ from stakeholder expectations Black-Right-Pointing-Pointer Considerable variability in influence of SEA output beyond licensing decisions Black-Right-Pointing-Pointer Sector-based SEA offshore is often too restrictive to generate expected benefits.« less

  17. On-line applications of numerical models in the Black Sea GIS

    NASA Astrophysics Data System (ADS)

    Zhuk, E.; Khaliulin, A.; Zodiatis, G.; Nikolaidis, A.; Nikolaidis, M.; Stylianou, Stavros

    2017-09-01

    The Black Sea Geographical Information System (GIS) is developed based on cutting edge information technologies, and provides automated data processing and visualization on-line. Mapserver is used as a mapping service; the data are stored in MySQL DBMS; PHP and Python modules are utilized for data access, processing, and exchange. New numerical models can be incorporated in the GIS environment as individual software modules, compiled for a server-based operational system, providing interaction with the GIS. A common interface allows setting the input parameters; then the model performs the calculation of the output data in specifically predefined files and format. The calculation results are then passed to the GIS for visualization. Initially, a test scenario of integration of a numerical model into the GIS was performed, using software, developed to describe a two-dimensional tsunami propagation in variable basin depth, based on a linear long surface wave model which is legitimate for more than 5 m depth. Furthermore, the well established oil spill and trajectory 3-D model MEDSLIK (http://www.oceanography.ucy.ac.cy/medslik/) was integrated into the GIS with more advanced GIS functionality and capabilities. MEDSLIK is able to forecast and hind cast the trajectories of oil pollution and floating objects, by using meteo-ocean data and the state of oil spill. The MEDSLIK module interface allows a user to enter all the necessary oil spill parameters, i.e. date and time, rate of spill or spill volume, forecasting time, coordinates, oil spill type, currents, wind, and waves, as well as the specification of the output parameters. The entered data are passed on to MEDSLIK; then the oil pollution characteristics are calculated for pre-defined time steps. The results of the forecast or hind cast are then visualized upon a map.

  18. Osage oil: Mineral law, murder, mayhem, and manipulation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Strickland, R.

    The greatest of the 20th century Osage chiefs, Fred Lookout, feared what the rich oil bonanza under tribal lands would do to his people. He forsaw that oil wealth could turn into a curse as well as a blessing, and it was both. The story of Osage oil is a case history in the failure of law, the failure of Indian policy and the struggle for survival of the indomitable spirit of a great Native people force to deal with both the curse and the blessing of black gold. This article examines law and policy as seen in Osage oilmore » regulation, outlining the legal controls of the land and mineral regulatory system and briefly exploring the breakdowns of the system.« less

  19. Oil recovery method using high water content oil-external micellar dispersions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jones, S.C.; Roszelle, W.O.; Svaldi, M.A.

    1971-10-19

    A high water content oil-external micellar dispersion (containing 55 percent to about 90 percent water) was developed for enhanced oil recovery. The micellar slug contained petroleum sulfonate (molecular weight averaged at about 350 to about 525), hydrocarbon, water and cosurfactant. The micellar slug was driven by a mobility buffer slug, which consisted of No. 530 Pusher, fusel oil and the residue Palestine water (420 ppm TDS) from the Palestine water reservoir in Palestine, Illinois. Fired Berea sandstone cores (porosity near 20 percent) were saturated with water (18,000 ppm sodium chloride), flooded with sweet black crude oil from Henry lease inmore » Illinois (7 cp at 72/sup 0/F), and waterflooded with water from Henry lease (18,000 ppm TDS). A maximum recovery of 11.5 percent of oil in place was recovered by 2 percent pore volume of a micellar dispersion containing petroleum sulfonate (MW 406), 70 percent by volume distilled water, and p-hexanol.« less

  20. High water content oil-external micellar dispersions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jones, S.C.; Roszelle, W.O.; Svaldi, M.A.

    1970-02-24

    A high water content oil-external micellar dispersion (containing 55 percent to about 90 percent water) was developed for enhanced oil recovery. The micellar slug contained petroleum sulfonate (molecular weight averaged at about 350 to about 525), hydrocarbon, water and cosurfactant. The micellar slug was driven by a mobility buffer slug, which consisted of No. 530 Pusher, fusel oil and the residue Palestine water (420 ppm TDS) from the Palestine water reservoir in Palestine, Illinois. Fired Berea sandstone cores (porosity near 20 percent) were saturated with water (18,000 ppm sodium chloride), flooded with sweet black crude oil from Henry lease inmore » Illinois (7 cp at 72/sup 0/F), and waterflooded with water from Henry lease (18,000 ppm TDS). A maximum recovery of 11.5 percent of oil in place was recovered by 2 percent pore volume of a micellar dispersion containing petroleum sulfonate (MW 406), crude oil, 70 percent by volume distilled water, and p-hexanol.« less

  1. Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae)

    PubMed Central

    2014-01-01

    Background Entomopathogenic fungi are potential candidates for use in integrated vector management, with recent emphasis aimed at developing adult mosquito control methods. Here we investigated the persistence of the fungus Metarhizium anisopliae when tested against female A. aegypti under field conditions. Methods Black cotton cloths impregnated with M. anisopliae conidia, formulated in vegetable oil + isoparaffin, were maintained on a covered veranda for up to 30 days. At specific times, pieces of the cloths were removed, placed in Tween 80 and the resuspended conidia were sprayed directly onto mosquitoes. The persistence of conidia impregnated on black cloths using three different carriers was evaluated in test rooms. Fifty mosquitoes were released into each room and after a 5 day period, the surviving insects were captured. Another 50 insects were then released into each room. The capacity of the fungus at reducing mosquito survival was evaluated over a total of 35 days. Results Conidia extracted from cloths maintained on the veranda for 2 to 18 days remained virulent, with 28 to 60% mosquito survival observed. Mosquito survival following exposure to fungus impregnated cloths showed that fungus + Tween caused similar reductions to that of fungus + vegetable oil. Mosquitoes exposed to the formulation fungus + vegetable oil had survival rates of 36% over the first 5 days of the experiment. Following the release of the second cohort of mosquitoes (6-11days), survival increased to 50%. The survival of the 12–17 day cohort (78%) was statistically equal to that of the controls (84%). Formulation of the fungus in vegetable oil + isoparaffin increased the persistence of the fungus, with the 18–23 day cohort (64% survival) still showing statistical differences to that of the controls (87% survival). Conclusions The potential of entomopathogenic fungi for the control of adult A. aegypti was confirmed under field conditions. Vegetable oil + isoparaffin formulations of M. anisopliae significantly increased the effectiveness of the fungus, thus reducing the need for frequent changes of black cloths in residences. Our future aim is to obtain effective control of mosquito populations, with cloths only needing to being replaced once a month. PMID:24766705

  2. Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae).

    PubMed

    Carolino, Aline T; Paula, Adriano R; Silva, Carlos P; Butt, Tariq M; Samuels, Richard I

    2014-04-25

    Entomopathogenic fungi are potential candidates for use in integrated vector management, with recent emphasis aimed at developing adult mosquito control methods. Here we investigated the persistence of the fungus Metarhizium anisopliae when tested against female A. aegypti under field conditions. Black cotton cloths impregnated with M. anisopliae conidia, formulated in vegetable oil + isoparaffin, were maintained on a covered veranda for up to 30 days. At specific times, pieces of the cloths were removed, placed in Tween 80 and the resuspended conidia were sprayed directly onto mosquitoes. The persistence of conidia impregnated on black cloths using three different carriers was evaluated in test rooms. Fifty mosquitoes were released into each room and after a 5 day period, the surviving insects were captured. Another 50 insects were then released into each room. The capacity of the fungus at reducing mosquito survival was evaluated over a total of 35 days. Conidia extracted from cloths maintained on the veranda for 2 to 18 days remained virulent, with 28 to 60% mosquito survival observed. Mosquito survival following exposure to fungus impregnated cloths showed that fungus + Tween caused similar reductions to that of fungus + vegetable oil. Mosquitoes exposed to the formulation fungus + vegetable oil had survival rates of 36% over the first 5 days of the experiment. Following the release of the second cohort of mosquitoes (6-11days), survival increased to 50%. The survival of the 12-17 day cohort (78%) was statistically equal to that of the controls (84%). Formulation of the fungus in vegetable oil + isoparaffin increased the persistence of the fungus, with the 18-23 day cohort (64% survival) still showing statistical differences to that of the controls (87% survival). The potential of entomopathogenic fungi for the control of adult A. aegypti was confirmed under field conditions. Vegetable oil + isoparaffin formulations of M. anisopliae significantly increased the effectiveness of the fungus, thus reducing the need for frequent changes of black cloths in residences. Our future aim is to obtain effective control of mosquito populations, with cloths only needing to being replaced once a month.

  3. Thermal maturity map of Devonian shale in the Illinois, Michigan, and Appalachian basins of North America

    USGS Publications Warehouse

    East, Joseph A.; Swezey, Christopher S.; Repetski, John E.; Hayba, Daniel O.

    2012-01-01

    Much of the oil and gas in the Illinois, Michigan, and Appalachian basins of eastern North America is thought to be derived from Devonian shale that is within these basins (for example, Milici and others, 2003; Swezey, 2002, 2008, 2009; Swezey and others, 2005, 2007). As the Devonian strata were buried by younger sediments, the Devonian shale was subjected to great temperature and pressure, and in some areas the shale crossed a thermal maturity threshold and began to generate oil. With increasing burial (increasing temperature and pressure), some of this oil-generating shale crossed another thermal maturity threshold and began to generate natural gas. Knowledge of the thermal maturity of the Devonian shale is therefore useful for predicting the occurrence and the spatial distribution of oil and gas within these three basins. This publication presents a thermal maturity map of Devonian shale in the Illinois, Michigan, and Appalachian basins. The map shows outlines of the three basins (dashed black lines) and an outline of Devonian shale (solid black lines). The basin outlines are compiled from Thomas and others (1989) and Swezey (2008, 2009). The outline of Devonian shale is a compilation from Freeman (1978), Thomas and others (1989), de Witt and others (1993), Dart (1995), Nicholson and others (2004), Dicken and others (2005a,b), and Stoeser and others (2005).

  4. 21 CFR 182.20 - Essential oils, oleoresins (solvent-free), and natural extractives (including distillates).

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Cananga Cananga odorata Hook. f. and Thoms. Capsicum Capsicum frutescens L. and Capsicum annuum L. Caraway... Stapf. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf. Pepper, black Piper nigrum...

  5. 21 CFR 182.20 - Essential oils, oleoresins (solvent-free), and natural extractives (including distillates).

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... Cananga Cananga odorata Hook. f. and Thoms. Capsicum Capsicum frutescens L. and Capsicum annuum L. Caraway... Stapf. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf. Pepper, black Piper nigrum...

  6. 21 CFR 182.20 - Essential oils, oleoresins (solvent-free), and natural extractives (including distillates).

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... Cananga Cananga odorata Hook. f. and Thoms. Capsicum Capsicum frutescens L. and Capsicum annuum L. Caraway... Stapf. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf. Pepper, black Piper nigrum...

  7. 21 CFR 182.20 - Essential oils, oleoresins (solvent-free), and natural extractives (including distillates).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... Cananga Cananga odorata Hook. f. and Thoms. Capsicum Capsicum frutescens L. and Capsicum annuum L. Caraway... Stapf. Paprika Capsicum annuum L. Parsley Petroselinum crispum (Mill.) Mansf. Pepper, black Piper nigrum...

  8. 21 CFR 177.1650 - Polysulfide polymer-polyepoxy resins.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...(2-chloroethyl) formal Bis(dichloropropyl) formal Cross-linking agent. Butyl alcohol Solvent. Carbon black (channel process) Chlorinated paraffins Cross-linking agent. Epoxidized linseed oil Epoxidized... monobutyl ether Solvent. Magnesium chloride Methyl isobutyl ketone Solvent. Naphthalene sulfonic acid...

  9. 40 CFR 63.6015 - What definitions apply to this subpart?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... finished tire for the purpose of sealing any future hole which might occur in the tread when an object... the sum of the materials (for example, natural rubber, synthetic rubber, carbon black, oils, sulfur...

  10. 40 CFR 63.6015 - What definitions apply to this subpart?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... finished tire for the purpose of sealing any future hole which might occur in the tread when an object... the sum of the materials (for example, natural rubber, synthetic rubber, carbon black, oils, sulfur...

  11. 40 CFR 63.6015 - What definitions apply to this subpart?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... finished tire for the purpose of sealing any future hole which might occur in the tread when an object... the sum of the materials (for example, natural rubber, synthetic rubber, carbon black, oils, sulfur...

  12. 40 CFR 63.6015 - What definitions apply to this subpart?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... finished tire for the purpose of sealing any future hole which might occur in the tread when an object... the sum of the materials (for example, natural rubber, synthetic rubber, carbon black, oils, sulfur...

  13. Advanced direct liquefaction concepts for PETC generic units. Quarterly technical progress report, October 1992--December 1992

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    The reactivity of the THF insoluble fraction of the ashy resid component of Wilsonville recycle oil (WRO) during liquefaction of Black Thunder coal in tetralin was determined at 415{degrees}C and 60 minutes. The liquefaction runs were made by combining this material with Black Thunder coal at the same ratio used in the WRO coal runs. THF conversion and product distribution from liquefaction in tetralin in the presence of the THF insoluble fraction of the ashy resid were similar to results from liquefaction in WRO. THF conversion was greater than loot with an oil yield that was somewhat higher than inmore » WRO. Differences in HC gas yield and H{sub 2} consumption were slight, while conversion and product distribution from liquefaction of Black Thunder coal in tetralin or in the WRO distillate were quite different. In both these solvents the 85--86% THF conversions were less than for runs in which the THF insoluble fraction of the ashy resid was present. This establishes that the THF insoluble fraction of the ashy resid is the reactive fraction of the WRO.« less

  14. Physicochemical and Antioxidant Properties of Rice Bran Oils Produced from Colored Rice Using Different Extraction Methods.

    PubMed

    Mingyai, Sukanya; Kettawan, Aikkarach; Srikaeo, Khongsak; Singanusong, Riantong

    2017-06-01

    This study investigated the physicochemical and antioxidant properties of rice bran oil (RBO) produced from the bran of three rice varities; Khao Dawk Mali 105 (white rice), Red Jasmine rice (red rice) and Hom-nin rice (black rice) using three extraction methods including cold-press extraction (CPE), solvent extraction (SE) and supercritical CO 2 extraction (SC-CO 2 ). Yields, color, acid value (AV), free fatty acid (FFA), peroxide value (PV), iodine value (IV), total phenolic compound (TPC), γ-oryzanol, α-tocopherol and fatty acid profile were analyzed. It was found that the yields obtained from SE, SC-CO 2 and CPE extractions were 17.35-20.19%, 14.76-18.16% and 3.22-6.22%, respectively. The RBO from the bran of red and black rice samples exhibited high antioxidant activities. They also contained higher amount of γ-oryzanol and α-tocopherol than those of white rice sample. In terms of extraction methods, SC-CO 2 provided better qualities of RBO as evidenced by their physicochemical and antioxidant properties. This study found that RBO produced from the bran of black rice samples using SC-CO 2 extraction method showed the best physicochemical and antioxidant properties.

  15. Assessment of metal contents in spices and herbs from Saudi Arabia.

    PubMed

    Seddigi, Z S; Kandhro, G A; Shah, F; Danish, E; Soylak, Mustafa

    2016-02-01

    In the recent years, there has been a growing interest in monitoring heavy metal contamination of spices/herbs. Spices and herbs are sources of many bioactive compounds that can improve the tastes of food as well as influence digestion and metabolism processes. In the present study, the levels of some essential and toxic elements such as iron (Fe), zinc (Zn), copper (Cu), chromium (Cr), manganese (Mn), cobalt (Co), nickel (Ni), lead (Pb), and cadmium (Cd), present in common spices/herbs that were purchased from the local market in Saudi Arabia, were analyzed by atomic absorption spectroscopy after digestion with nitric acid/hydrogen peroxide mixture. Samples from the following spices/herbs were used: turmeric, cloves, black pepper, red pepper, cumin, legume, cinnamon, abazir, white pepper, ginger, and coriander. The concentration ranges for the studied elements were found as 48.8-231, 4.7-19.4, 2.5-10.5, below detection level (BDL)-1.0, 8.8-490, 1.0-2.6, and BDL-3.7 µg g(-1) for Fe, Zn, Cu, Cr, Mn, Ni, and Pb, respectively, while Cd and Co levels were below the detection limit. Consumers of these spices/herbs would not be exposed to any risk associated with the daily intake of 10 g of spices per day as far as metals Fe, Zn, Cu, Cr, Mn, Ni, and Pb are concerned. © The Author(s) 2013.

  16. Studies on optical and electrical properties of green synthesized TiO2@Ag core-shell nanocomposite material

    NASA Astrophysics Data System (ADS)

    Ganapathy, M.; Senthilkumar, N.; Vimalan, M.; Jeysekaran, R.; Vetha Potheher, I.

    2018-04-01

    Newly adopted green approach has been used to synthesize pure titanium dioxide (TiO2) nanoparticles (NPs) and silver deposited titanium dioxide (TiO2@Ag) core–shell nanocomposite (CSNC) from Nigella Sativa (black cumin) seed extract for the first time. The phytochemicals available in Nigella Sativa (NS) seed extract acts as reducing agent in the formation of nanoparticles as well as nanocomposite. The morphology, crystal structure, particle size and phase composition of green synthesized TiO2 NPs and TiO2@Ag CSNC are investigated by High Resolution Transmission Electron Microscopy (HRTEM), Field Emission Scanning Electron Microscopy (FESEM), Powder x-ray diffraction (PXRD), FT–Raman and Fourier Transform Infrared spectroscopy (FT-IR). The red shift in (from 333 nm to 342 nm) UV–Vis spectrum confirmed the deposition of Ag on TiO2. The reduced intensity peaks of Photoluminescence spectra (PL) also indicate the deposition of Ag on TiO2. Further the electrical properties of pure TiO2 and TiO2@Ag CSNC have studied by dielectric studies and ac conductivity measurements. The dielectric constant and the dielectric loss of TiO2@Ag CSNC are better than pure TiO2. From these improved results, the green synthesized TiO2@Ag CSNC from NS seed extract is may be a suitable material for device fabrication in the visible region.

  17. Protective effect of thymoquinone improves cardiovascular function, and attenuates oxidative stress, inflammation and apoptosis by mediating the PI3K/Akt pathway in diabetic rats.

    PubMed

    Liu, Hui; Liu, Hong-Yang; Jiang, Yi-Nong; Li, Nan

    2016-03-01

    Thymoquinone is the main active monomer extracted from black cumin and has anti‑inflammatory, antioxidant and anti‑apoptotic functions. However, the protective effects of thymoquinone on cardiovascular function in diabetes remain to be fully elucidated. The present study aimed to investigate the molecular mechanisms underling the beneficial effects of thymoquinone on the cardiovascular function in streptozotocin‑induced diabetes mellitus (DM) rats. Supplement thymoquinone may recover the insulin levels and body weight, inhibit blood glucose levels and reduce the heart rate in DM‑induced rats. The results indicated that the heart, liver and lung to body weight ratios, in addition to the blood pressure levels, were similar for each experimental group. Treatment with thymoquinone significantly reduced oxidative stress damage, inhibited the increased endothelial nitric oxide synthase protein expression and suppressed the elevation of cyclooxygenase‑2 levels in DM‑induced rats. In addition, thymoquinone significantly suppressed the promotion of tumor necrosis factor‑α and interleukin‑6 levels in the DM‑induced rats. Furthermore, administration of thymoquinone significantly reduced caspase‑3 activity and the promotion of phosphorylated‑protein kinase B (Akt) protein expression levels in DM‑induced rats. These results suggest that the protective effect of thymoquinone improves cardiovascular function and attenuates oxidative stress, inflammation and apoptosis by mediating the phosphatidylinositol 3‑kinase/Akt pathway in DM‑induced rats.

  18. Organic and inorganic fertilizer effect on soil CO2 flux, microbial biomass, and growth of Nigella sativa L.

    NASA Astrophysics Data System (ADS)

    Salehi, Aliyeh; Fallah, Seyfollah; Sourki, Ali Abasi

    2017-01-01

    Cattle manure has a high carbon/nitrogen ratio and may not decompose; therefore, full-dose application of urea fertilizer might improve biological properties by increasing manure decomposition. This study aimed to investigate the effect of combining cattle manure and urea fertilizer on soil CO2 flux, microbial biomass carbon, and dry matter accumulation during Nigella sativa L. (black cumin) growth under field conditions. The treatments were control, cattle manure, urea, different levels of split and full-dose integrated fertilizer. The results showed that integrated application of cattle manure and chemical fertilizer significantly increased microbial biomass carbon by 10%, soil organic carbon by 2.45%, total N by 3.27%, mineral N at the flowering stage by 7.57%, and CO2 flux by 9% over solitary urea application. Integrated application increased microbial biomass carbon by 10% over the solitary application and the full-dose application by 5% over the split application. The soil properties and growth parameters of N. sativa L. benefited more from the full-dose application than the split application of urea. Cattle manure combined with chemical fertilizer and the full-dose application of urea increased fertilizer efficiency and improved biological soil parameters and plant growth. This method decreased the cost of top dressing urea fertilizer and proved beneficial for the environment and medicinal plant health.

  19. Immunity: plants as effective mediators.

    PubMed

    Sultan, M Tauseef; Butt, Masood Sadiq; Qayyum, Mir M Nasir; Suleria, Hafiz Ansar Rasul

    2014-01-01

    In the domain of nutrition, exploring the diet-health linkages is major area of research. The outcomes of such interventions led to widespread acceptance of functional and nutraceutical foods; however, augmenting immunity is a major concern of dietary regimens. Indeed, the immune system is incredible arrangement of specific organs and cells that enabled humans to carry out defense against undesired responses. Its proper functionality is essential to maintain the body homeostasis. Array of plants and their components hold immunomodulating properties. Their possible inclusion in diets could explore new therapeutic avenues to enhanced immunity against diseases. The review intended to highlight the importance of garlic (Allium sativum), green tea (Camellia sinensis), ginger (Zingiber officinale), purple coneflower (Echinacea), black cumin (Nigella sativa), licorice (Glycyrrhiza glabra), Astragalus and St. John's wort (Hypericum perforatum) as natural immune boosters. These plants are bestowed with functional ingredients that may provide protection against various menaces. Modes of their actions include boosting and functioning of immune system, activation and suppression of immune specialized cells, interfering in several pathways that eventually led to improvement in immune responses and defense system. In addition, some of these plants carry free radical scavenging and anti-inflammatory activities that are helpful against cancer insurgence. Nevertheless, interaction between drugs and herbs/botanicals should be well investigated before recommended for their safe use, and such information must be disseminated to the allied stakeholders.

  20. A new method for determination of cocamidopropyl betaine synthesized from coconut oil through spectral shift of Eriochrome Black T

    NASA Astrophysics Data System (ADS)

    Gholami, Ali; Golestaneh, Mahshid; Andalib, Zeinab

    2018-03-01

    Cocamidopropyl betaine (CAPB) is a zwitterionic surfactant that is synthesized using coconut oil and usually supplied in form of an aqueous solution with 25-37% w/w. In this study, a novel method based on UV-visible spectroscopy is developed for an accurate determination of CAPB synthesized from coconut oil. Eriochrome Black T (EBT) as a specific color indicator was added to CAPB and a red shift and color change were observed. This shift leads in increasing wavelength selectivity of the method. The change in the color intensity depends on the concentration of CAPB. By measuring the absorbance of a solution containing CAPB, its concentration was measured. After optimizing all the effective parameters, CAPB was detected in commercial real samples. Using the proposed approach, limit of quantification (LOQ) and relative standard deviation (RSD) were obtained about 4.30 × 10- 5 M and 4.8% respectively. None of unreacted materials or by-products, which were produced in the synthesis of CAPB, showed any interference in the determination of CAPB. This shows that the proposed method is specific and accurate, and can potentially be used for quantitative determination of CAPB in commercial samples with satisfactory results.

  1. Effect of dietary antioxidant supplementation (Cuminum cyminum) on bacterial susceptibility of diabetes-induced rats.

    PubMed

    Moubarz, Gehan; Embaby, Mohamed A; Doleib, Nada M; Taha, Mona M

    2016-01-01

    Diabetic patients are at risk of acquiring infections. Chronic low-grade inflammation is an important factor in the pathogenesis of diabetic complication. Diabetes causes generation of reactive oxygen species that increases oxidative stress, which may play a role in the development of complications as immune-deficiency and bacterial infection. The study aimed to investigate the role of a natural antioxidant, cumin, in the improvement of immune functions in diabetes. Diabetes was achieved by interperitoneal injection of streptozotocin (STZ). Bacterial infection was induced by application of Staphylococcus aureus suspension to a wound in the back of rats. The antioxidant was administered for 6 weeks. Results revealed a decrease in blood glucose levels in diabetic rats (p < 0.001), in addition to improving immune functions by decreasing total IgE approaching to the normal control level. Also, inflammatory cytokine (IL-6, IL-1β and TNF) levels, as well as total blood count decreased in diabetic rats as compared to the control group. Thus, cumin may serve as anti-diabetic treatment and may help in attenuating diabetic complications by improving immune functions. Therefore, a medical dietary antioxidant supplementation is important to improve the immune functions in diabetes.

  2. Effect of dietary antioxidant supplementation (Cuminum cyminum) on bacterial susceptibility of diabetes-induced rats

    PubMed Central

    Embaby, Mohamed A.; Doleib, Nada M.; Taha, Mona M.

    2016-01-01

    Diabetic patients are at risk of acquiring infections. Chronic low-grade inflammation is an important factor in the pathogenesis of diabetic complication. Diabetes causes generation of reactive oxygen species that increases oxidative stress, which may play a role in the development of complications as immune-deficiency and bacterial infection. The study aimed to investigate the role of a natural antioxidant, cumin, in the improvement of immune functions in diabetes. Diabetes was achieved by interperitoneal injection of streptozotocin (STZ). Bacterial infection was induced by application of Staphylococcus aureus suspension to a wound in the back of rats. The antioxidant was administered for 6 weeks. Results revealed a decrease in blood glucose levels in diabetic rats (p < 0.001), in addition to improving immune functions by decreasing total IgE approaching to the normal control level. Also, inflammatory cytokine (IL-6, IL-1β and TNF) levels, as well as total blood count decreased in diabetic rats as compared to the control group. Thus, cumin may serve as anti-diabetic treatment and may help in attenuating diabetic complications by improving immune functions. Therefore, a medical dietary antioxidant supplementation is important to improve the immune functions in diabetes. PMID:27536197

  3. CHEMICAL RECLAMATION OF SCRAP RUBBER

    EPA Science Inventory

    A conceptual, commercial-scale plant design was formulated for processing 22,500 t/yr of scrap rubber tires to hydrocarbon fuel gases, oils, petrochemicals (principally ethylene and aromatic liquids), and carbon black. The process is based upon molten salt (zinc chloride) pyrolys...

  4. 40 CFR 428.11 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... AND STANDARDS (CONTINUED) RUBBER MANUFACTURING POINT SOURCE CATEGORY Tire and Inner Tube Plants... black, oils, chemical compounds, fabric and wire used in the manufacture of pneumatic tires and inner... inner tube plants constructed before 1959, discharges from the following: Soapstone solution...

  5. 40 CFR 428.11 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... AND STANDARDS (CONTINUED) RUBBER MANUFACTURING POINT SOURCE CATEGORY Tire and Inner Tube Plants... black, oils, chemical compounds, fabric and wire used in the manufacture of pneumatic tires and inner... inner tube plants constructed before 1959, discharges from the following: Soapstone solution...

  6. Central Saudi Arabia, Persian Gulf

    NASA Technical Reports Server (NTRS)

    1992-01-01

    This panoramic view of Central Saudi Arabia and the Persian Gulf (28.0N, 47.0E) dramatically illustrates the stark beauty of the deserts. Riyadh, the capital city, lies in the foreground, with the Persian Gulf in the middle and Iran in the background. The coastal oil terminals of Al Hufuf and Ad Dammam are also visible. Black smudges of soot from the oil fires set during the Gulf War can be seen on the sands to the north and south of Kuwait City.

  7. Aflatoxin-induced biochemical changes in liver of mice and its mitigation by black tea extract.

    PubMed

    Jha, Anamika; Shah, Komal; Verma, Ramtej J

    2012-01-01

    Aflatoxin belongs to the class of naturally occurring mycotoxins, food contaminants having potent carcinogenicity. We have evaluated the ameliorative role of black tea extract on aflatoxin-induced biochemical changes in the liver of albino male mice. Adult male mice were orally administered with 750 and 1500 pg of aflatoxin in 0.2 mL olive oil/kg b.w./day for 30 days. Oral administration of aflatoxin caused, as compared with controls, significant, dose-dependent reduction in DNA, RNA, protein and glycogen contents; however, cholesterol content and phsphorylase activity were significantly increased. Black tea is one of the most potent antioxidants containing numerous bioactive phytonurtients having therapeutic applications. Aflatoxin-induced changes in the liver of mice were significantly ameliorated on co-treatment of black tea extract (2% infusion in water).

  8. Characterization of cellulosic wastes and gasification products from chicken farms

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Joseph, Paul, E-mail: p.joseph@ulster.ac.uk; Tretsiakova-McNally, Svetlana; McKenna, Siobhan

    Highlights: Black-Right-Pointing-Pointer The gas chromatography indicated the variable quality of the producer gas. Black-Right-Pointing-Pointer The char had appreciable NPK values, and can be used as a fertiliser. Black-Right-Pointing-Pointer The bio-oil produced was of poor quality, having high moisture content and low pH. Black-Right-Pointing-Pointer Mass and energy balances showed inadequate level energy recovery from the process. Black-Right-Pointing-Pointer Future work includes changing the operating parameters of the gasification unit. - Abstract: The current article focuses on gasification as a primary disposal solution for cellulosic wastes derived from chicken farms, and the possibility to recover energy from this process. Wood shavings and chickenmore » litter were characterized with a view to establishing their thermal parameters, compositional natures and calorific values. The main products obtained from the gasification of chicken litter, namely, producer gas, bio-oil and char, were also analysed in order to establish their potential as energy sources. The experimental protocol included bomb calorimetry, pyrolysis combustion flow calorimetry (PCFC), thermo-gravimetric analyses (TGA), differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, elemental analyses, X-ray diffraction (XRD), mineral content analyses and gas chromatography. The mass and energy balances of the gasification unit were also estimated. The results obtained confirmed that gasification is a viable method of chicken litter disposal. In addition to this, it is also possible to recover some energy from the process. However, energy content in the gas-phase was relatively low. This might be due to the low energy efficiency (19.6%) of the gasification unit, which could be improved by changing the operation parameters.« less

  9. Chemistry and in vitro antioxidant activity of volatile oil and oleoresins of black pepper (Piper nigrum).

    PubMed

    Kapoor, I P S; Singh, Bandana; Singh, Gurdip; De Heluani, Carola S; De Lampasona, M P; Catalan, Cesar A N

    2009-06-24

    Essential oil and oleoresins (ethanol and ethyl acetate) of Piper nigrum were extracted by using Clevenger and Soxhlet apparatus, respectively. GC-MS analysis of pepper essential oil showed the presence of 54 components representing about 96.6% of the total weight. beta-Caryophylline (29.9%) was found as the major component along with limonene (13.2%), beta-pinene (7.9%), sabinene (5.9%), and several other minor components. The major component of both ethanol and ethyl acetate oleoresins was found to contain piperine (63.9 and 39.0%), with many other components in lesser amounts. The antioxidant activities of essential oil and oleoresins were evaluated against mustard oil by peroxide, p-anisidine, and thiobarbituric acid. Both the oil and oleoresins showed strong antioxidant activity in comparison with butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) but lower than that of propyl gallate (PG). In addition, their inhibitory action by FTC method, scavenging capacity by DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), and reducing power were also determined, proving the strong antioxidant capacity of both the essential oil and oleoresins of pepper.

  10. Persistency of larvicidal effects of plant oil extracts under different storage conditions.

    PubMed

    Amer, Abdelkrim; Mehlhorn, Heinz

    2006-09-01

    The persistency of larvicidal effects of 13 oils (camphor, thyme, amyris, lemon, cedarwood, frankincense, dill, myrtle, juniper, black pepper, verbena, helichrysum, and sandalwood) was examined by storage of 50-ppm solutions under different conditions (open, closed, in the light, and in the dark) for 1 month after the preparation of the solutions. The stored solutions were tested against Aedes aegypti larvae for four times during the storage period. Some oils under some conditions stayed effective until the last test, while some solutions had lost their toxicity during a short time after preparation. Thus, the mode of storage is absolutely important for the larvicidal effects. The fresh preparations were always the best.

  11. Pigment Identification on "The Ecstasy of St. Theresa" Painting by Raman Microscopy

    NASA Astrophysics Data System (ADS)

    Marano, D.; Marmontelli, M.; De Benedetto, G. E.; Catalano, I. M.; Sabbatini, L.; Vona, F.

    A study of the pigments of "The Ecstasy of St. Theresa," a seventeenth century oil painting on canvas, was performed by Raman microscopy. Lazurite was identified in both Jesus Christ's and St. Theresa's mantles as the pigment responsible for the blue coloration. Litharge was identified inside the black bitumen layer. Usually the bitumen needed a lot of time to dry in the air when mixed with drying oil. Litharge was used by the artist to decrease the oil drying time. A complementary study, using micro-Raman and SEM, allowed us to identify red ochre as the pigment responsible for the red coloration in the altar on the left side of the painting.

  12. Genome Sequence of Salt-Tolerant Bacillus safensis Strain VK, Isolated from Saline Desert Area of Gujarat, India.

    PubMed

    Kothari, V V; Kothari, R K; Kothari, C R; Bhatt, V D; Nathani, N M; Koringa, P G; Joshi, C G; Vyas, B R M

    2013-09-05

    Bacillus safensis strain VK was isolated from the rhizosphere of a cumin plant growing in the saline desert of Radhanpar, Gujarat, India. Here, we provide the 3.68-Mb draft genome sequence of B. safensis VK, which might provide information about the salt tolerance and genes encoding enzymes for the strain's plant growth-promoting potential.

  13. An Application of Singularity Analysis to a Heavy Precipitation Event

    DTIC Science & Technology

    1993-01-01

    difference in this plot. 61 NORMAN RADAR ELEVATION: 3.094 DATE 28 MAY 87 TIM 005342 GUT Cumin a 10 Tpam c CD low am am Io CID e0UC 100 "CC C d~Cc 100...element of fluid shaped like a sphere. Assuming the fluid is friction- less, no tangential stresses or forces are applied to its surface. The pressure

  14. Environmental Assessment for Restoration and Stabilization of Eastern Shoreline MacDill AFB, Florida

    DTIC Science & Technology

    2005-12-01

    heal stress , and machinery injuries. Construction would not involve any unique hazards and all construction methods would comply with Occupational...slips, heat stress , and machinery injuries. Construction would not involve any unique hazards, and all construction methods would comply with OSHA...Chloropropane, Benzene, Cumine , Naphthalene, benzo(b)flouranthene, toluene Soils: Benzo(a)pyrene, dibenzo(a,h)anthracene, Pb, naphthalene, toluene Surface

  15. Minimum Nuclear Deterrence Research

    DTIC Science & Technology

    2003-05-15

    well as by additional experts interviewed in Paris (November 2002). One expert at the French MoD stressed the psychological importance of the 1940...continued to stress France’s commitment to "contributing to European security" specifically through the existence of its nuclear deterrent.43...Mondiale (Thesis, Paris, Librairie Générale de Droit et de Jurisprudence, 1966, p. 441) as cited in David Cumin , L’Arme

  16. Impact of commercial garden growth substratum and NPK-fertilizer on copper fractionation in a copper-mine tailing

    NASA Astrophysics Data System (ADS)

    Charles, A.; Karam, A.; Jaouich, A.

    2009-04-01

    Organic amendment and NPK-fertilizer could affect the distribution of copper (Cu) among Cu-mine tailing compounds and hence the availability or phytotoxicity of Cu to plants. A laboratory incubation experiment was conducted to investigate the forms of Cu in a Cu-mine tailing (pH 7.70) amended with a commercial garden growth substratum (GGS) containing peat moss and natural mycorrhizae (Glomus intraradices) in combination with a commercial NPK-fertilizer (20-20-20), by a sequential extraction method. There were eight treatments after the combination of four rates of GGS (0, 12.4, 50 and 100 g/kg tailing) and two rates of fertilizer (0 and 20 g/kg tailing). At the end of a 52-week incubation period, tailing Cu was sequentially extracted to fractionate Cu into five operationally defined geochemical forms, namely ‘water-soluble' (Cu-sol), ‘exchangeable' (Cu-exc), ‘specifically adsorbed on carbonates or carbonate-bound' (Cu-car), ‘organic-bound' (Cu-org) and ‘residual' (Cu-res) fractions. After treatments, the most labile Cu pool (Cu-sol + Cu-exc) represented about 0.94 % of the total Cu, the Cu-car and Cu-org accounted for 22.7 and 5.0% of total Cu, and the residual Cu accounted for nearly 71.3% of total Cu. Compared with the control, the application of GGS decreased Cu-car and increased CuORG whereas the addition of fertilizer increased Cu-sol + Cu-exc and decreased Cu-carb. Fertilizer-treated tailings had the highest amount of Cu-sol + Cu-exc. High rates of GGS resulted in Cu-org levels in GGS-treated tailings which were more than 2.0-2.8 times those obtained in the untreated tailing (control). The partition of Cu in GGS-treated tailings followed the order: Cu-sol + Cu-exc < Cu-car < Cu-org < Cu-res. This study suggests that NPK-fertilizer promotes the formation of labile Cu forms in the calcite-containing Cu-mine tailing. GGS in the tailing matrix acts as effective sorbent for Cu.

  17. Identification of potent odorants formed during the preparation of extruded potato snacks.

    PubMed

    Majcher, Małgorzata A; Jeleń, Henryk H

    2005-08-10

    Extrusion cooking processing followed by air-drying has been applied to obtain low-fat potato snacks. Optimal parameters were developed for a dough recipe. Dough contained apart from potato granules 7% of canola oil, 1% of salt, 1% of baking powder, 5% of maltodextrin, and 15% of wheat flour. After the extrusion process, snacks were dried at 85 degrees C for 15 min followed by 130 degrees C for 45 min. The potent odorants of extruded potato snacks were identified using aroma extract dilution analysis and gas chromatography-olfactometry. Among the characteristic compounds, methional with boiled potato flavor, benzenemethanethiol with pepper-seed flavor, 2-acetyl-1-pyrroline with popcorn flavor, benzacetaldehyde with strong flowery flavor, butanal with rancid flavor, and 2-acetylpyrazine with roasty flavor were considered to be the main contributors to the aroma of extruded potato snacks. Several compounds were concluded to be developed during extrusion cooking, such as ethanol, 3-methylbutanal, (Z)-1,5-octadien-3-one with geranium flavor, and unknown ones with the flavor of boiled potato, cumin, candy, or parsley root. Compounds such as methanethiol, 2,3-pentanedione, limonene, 2-acetylpyrazine, 2-ethyl-3,5-dimethylpyrazine, 4-hydroxy-2,5-dimethyl-3(2H)-furanone, 3-hydroxy-4,5-dimethyl-2(5H)-furanone, 2-methyl-3,5-diethylpyrazine, 5-methyl-2,3-diethylpyrazine, and (E)-beta-damascenone were probably developed during air-drying of the potato extrudate.

  18. 40 CFR 180.593 - Etoxazole; tolerances for residues.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Papaya 0.20 Pepper/eggplant subgroup 8-10B 0.20 Peppermint, oil 20 Peppermint, tops 10 Pistachio 0.01 Plum 0.15 Plum, prune, dried 0.30 Sapodilla 0.20 Sapote, black 0.20 Sapote, mamey 0.20 Sheep, fat 0.02...

  19. Thermal maturity patterns (conodont color alteration index and vitrinite reflectance) in Upper Ordovician and Devonian rocks of the Appalachian basin: a major revision of USGS Map I-917-E using new subsurface collections: Chapter F.1 in Coal and petroleum resources in the Appalachian basin: distribution, geologic framework, and geochemical character

    USGS Publications Warehouse

    Repetski, John E.; Ryder, Robert T.; Weary, David J.; Harris, Anita G.; Trippi, Michael H.; Ruppert, Leslie F.; Ryder, Robert T.

    2014-01-01

    The conodont color alteration index (CAI) introduced by Epstein and others (1977) and Harris and others (1978) is an important criterion for estimating the thermal maturity of Ordovician to Mississippian rocks in the Appalachian basin. Consequently, the CAI isograd maps of Harris and others (1978) are commonly used by geologists to characterize the thermal and burial history of the Appalachian basin and to better understand the origin and distribution of oil and gas resources in the basin. The main objectives of this report are to present revised CAI isograd maps for Ordovician and Devonian rocks in the Appalachian basin and to interpret the geologic and petroleum resource implications of these maps. The CAI isograd maps presented herein complement, and in some areas replace, the CAI-based isograd maps of Harris and others (1978) for the Appalachian basin. The CAI data presented in this report were derived almost entirely from subsurface samples, whereas the CAI data used by Harris and others (1978) were derived almost entirely from outcrop samples. Because of the different sampling methods, there is little geographic overlap of the two data sets. The new data set is mostly from the Allegheny Plateau structural province and most of the data set of Harris and others (1978) is from the Valley and Ridge structural province, east of the Allegheny structural front (fig. 1). Vitrinite reflectance, based on dispersed vitrinite in Devonian black shale, is another important parameter for estimating the thermal maturity in pre-Pennsylvanian-age rocks of the Appalachian basin (Streib, 1981; Cole and others, 1987; Gerlach and Cercone, 1993; Rimmer and others, 1993; Curtis and Faure, 1997). This chapter also presents a revised percent vitrinite reflectance (%R0) isograd map based on dispersed vitrinite recovered from selected Devonian black shales. The Devonian black shales used for the vitrinite studies reported herein also were analyzed by RockEval pyrolysis and total organic carbon (TOC) content in weight percent. Although the RockEval and TOC data are included in this chapter (table 1), they are not shown on the maps. The revised CAI isograd and percent vitrinite reflectance isograd maps cover all or parts of Kentucky, New York, Ohio, Pennsylvania, Virginia, and West Virginia (fig. 1), and the following three stratigraphic intervals: Upper Ordovician carbonate rocks, Lower and Middle Devonian carbonate rocks, and Middle and Upper Devonian black shales. These stratigraphic intervals were chosen for the following reasons: (1) they represent target reservoirs for much of the oil and gas exploration in the Appalachian basin; (2) they are stratigraphically near probable source rocks for most of the oil and gas; (3) they include geologic formations that are nearly continuous across the basin; (4) they contain abundant carbonate grainstone-packstone intervals, which give a reasonable to good probability of recovery of conodont elements from small samples of drill cuttings; and (5) the Middle and Upper Devonian black shale contains large amounts of organic matter for RockEval, TOC, and dispersed vitrinite analyses. Thermal maturity patterns of the Upper Ordovician Trenton Limestone are of particular interest here, because they closely approximate the thermal maturity patterns in the overlying Upper Ordovician Utica Shale, which is the probable source rock for oil and gas in the Upper Cambrian Rose Run Sandstone (sandstone), Upper Cambrian and Lower Ordovician Knox Group (Dolomite), Lower and Middle Ordovician Beekmantown Group (dolomite or Dolomite), Upper Ordovician Trenton and Black River Limestones, and Lower Silurian Clinton/Medina sandstone (Cole and others, 1987; Jenden and others, 1993; Laughrey and Baldassare, 1998; Ryder and others, 1998; Ryder and Zagorski, 2003). The thermal maturity patterns of the Lower Devonian Helderberg Limestone (Group), Middle Devonian Onondaga Limestone, and Middle Devonian Marcellus Shale-Upper Devonian Rhine street Shale Member-Upper Devonian Ohio Shale are of interest, because they closely approximate the thermal maturity patterns in the Marcellus Shale, Upper Devonian Rhinestreet Shale Member, and Upper Devonian Huron Member of the Ohio Shale, which are the most important source rocks for oil and gas in the Appalachian basin (de Witt and Milici, 1989; Klemme and Ulmishek, 1991). The Marcellus, Rhinestreet, and Huron units are black-shale source rocks for oil and (or) gas in the Lower Devonian Oriskany Sandstone, the Upper Devonian sandstones, the Middle and Upper Devonian black shales, and the Upper Devonian-Lower Mississippian(?) Berea Sandstone (Patchen and others, 1992; Roen and Kepferle, 1993; Laughrey and Baldassare, 1998).

  20. Mineral resources of the Little Black Peak and Carrizozo Lava Flow wilderness study areas, Lincoln County, New Mexico

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stoeser, D.B.; Senterfit, M.K.; Zelten, J.E.

    1989-01-01

    This book discusses the Little Black Peak and Carrizozo Lava Flow Wilderness Study Areas in east-central New Mexico (24,249 acres) which are underlain by Quaternary basaltic lava flows and upper Paleozoic to Mesozoic sedimentary rocks. The only identified resource is lava from the basalt flows, which is used for road metal, construction materials, and decorative stone. The basalt is classed as an inferred subeconomic resource. Both areas have low resource potential for sediment-hosted uranium and copper oil, gas, coal, and geothermal energy and moderate potential for gypsum and salt. The Little Black Peak area also has low potential for uraniummore » associated with Tertiary alkaline intrusive rocks. Two aeromagnetic anomalies occur beneath the northern part of the Carrizozo lava flow area and the southern part of the Little Black Peak area; the resource potential for these rocks is unknown.« less

  1. Biodegradable Chitosan Coating Incorporated with Black Pepper Essential Oil for Shelf Life Extension of Common Carp (Cyprinus carpio) during Refrigerated Storage.

    PubMed

    Moosavi-Nasab, Marzieh; Shad, Ehsan; Ziaee, Esmaeil; Yousefabad, Seyyed Hossein Asadi; Golmakani, Mohammad Taghi; Azizinia, Mehdi

    2016-06-01

    Chitosan (Ch) coating incorporated with black pepper essential oil (Ch+BPEO) was studied to extend the shelf life of common carp (Cyprinus carpio) during refrigerated storage at 4 ± 1°C. The chemical composition of BPEO was characterized using gas chromatography-mass spectrometry (GC-MS). Antibacterial properties of BPEO were determined by disk diffusion agar, MIC, and MBC. Ch (2% [wt/vol]) and Ch+BPEO (2% [wt/vol] Ch with 1.5% [vol/vol] BPEO) were used for common carp fillet coating. The samples were analyzed periodically for chemical (pH, total volatile basic nitrogen) and microbiological (aerobic plate count, psychrophilic bacteria count, lactic acid bacteria, and Enterobacteriaceae bacterial counts) characteristics during 16 days. The GC-MS results indicated that main components in BPEO were carene, caryophyllene, limonene, β-pinene, and α-pinene. The samples coated with Ch and Ch+BPEO resulted in lower pH and total volatile basic nitrogen values in comparison with the control. The microbiological analysis of fish fillets during refrigerated storage clearly indicated that Ch+BPEO coating significantly reduced the fish fillet microbial load. The aerobic plate count, psychrophilic bacteria count, lactic acid bacteria count, and Enterobacteriaceae bacterial count of samples coated with Ch+BPEO were reduced approximately 4.1, 3.9, 2.3, and 2.8 log CFU/g, respectively, at the end of the storage period. Finally, Ch and Ch+BPEO effectively improved the quality of fish fillet during refrigerated storage and extended the shelf life of fish fillets from 8 to 16 days. Black pepper; Chitosan; Common carp; Essential oil.

  2. Control of Origin of Sesame Oil from Various Countries by Stable Isotope Analysis and DNA Based Markers—A Pilot Study

    PubMed Central

    Horacek, Micha; Hansel-Hohl, Karin; Burg, Kornel; Soja, Gerhard; Okello-Anyanga, Walter; Fluch, Silvia

    2015-01-01

    The indication of origin of sesame seeds and sesame oil is one of the important factors influencing its price, as it is produced in many regions worldwide and certain provenances are especially sought after. We joined stable carbon and hydrogen isotope analysis with DNA based molecular marker analysis to study their combined potential for the discrimination of different origins of sesame seeds. For the stable carbon and hydrogen isotope data a positive correlation between both isotope parameters was observed, indicating a dominant combined influence of climate and water availability. This enabled discrimination between sesame samples from tropical and subtropical/moderate climatic provenances. Carbon isotope values also showed differences between oil from black and white sesame seeds from identical locations, indicating higher water use efficiency of plants producing black seeds. DNA based markers gave independent evidence for geographic variation as well as provided information on the genetic relatedness of the investigated samples. Depending on the differences in ambient environmental conditions and in the genotypic fingerprint, a combination of both analytical methods is a very powerful tool to assess the declared geographic origin. To our knowledge this is the first paper on food authenticity combining the stable isotope analysis of bio-elements with DNA based markers and their combined statistical analysis. PMID:25831054

  3. Control of origin of sesame oil from various countries by stable isotope analysis and DNA based markers--a pilot study.

    PubMed

    Horacek, Micha; Hansel-Hohl, Karin; Burg, Kornel; Soja, Gerhard; Okello-Anyanga, Walter; Fluch, Silvia

    2015-01-01

    The indication of origin of sesame seeds and sesame oil is one of the important factors influencing its price, as it is produced in many regions worldwide and certain provenances are especially sought after. We joined stable carbon and hydrogen isotope analysis with DNA based molecular marker analysis to study their combined potential for the discrimination of different origins of sesame seeds. For the stable carbon and hydrogen isotope data a positive correlation between both isotope parameters was observed, indicating a dominant combined influence of climate and water availability. This enabled discrimination between sesame samples from tropical and subtropical/moderate climatic provenances. Carbon isotope values also showed differences between oil from black and white sesame seeds from identical locations, indicating higher water use efficiency of plants producing black seeds. DNA based markers gave independent evidence for geographic variation as well as provided information on the genetic relatedness of the investigated samples. Depending on the differences in ambient environmental conditions and in the genotypic fingerprint, a combination of both analytical methods is a very powerful tool to assess the declared geographic origin. To our knowledge this is the first paper on food authenticity combining the stable isotope analysis of bio-elements with DNA based markers and their combined statistical analysis.

  4. Buying success, Saudi style

    NASA Astrophysics Data System (ADS)

    Durrani, Matin

    2009-11-01

    Oil - it is what has made Saudi Arabia rich. Without this black gold, none of the country's shopping malls, luxury hotels, six-lane highways or the airconditioned comfort that keeps the terrible heat at bay would be possible. From the first deposits harvested in the early 1950s, Saudi Arabia is now the world's leading exporter of petroleum, which accounts for nearly three-quarters of government revenue. And without the wealth from oil, the huge new King Abdullah University of Science and Technology (KAUST), built on hard, desert soil in less than two years, would have been impossible.

  5. Inventory and evaluation of potential oil shale development in Kansas

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Angino, E.; Berg, J.; Dellwig, L.

    The University of Kansas Center for Research, Inc. was commissioned by the Kansas Energy Office and the US Department of Energy to conduct a review of certain oil shales in Kansas. The purpose of the study focused on making an inventory and assessing those oil shales in close stratigraphic proximity to coal beds close to the surface and containing significant reserves. The idea was to assess the feasibility of using coal as an economic window to aid in making oil shales economically recoverable. Based on this as a criterion and the work of Runnels, et al., (Runnels, R.T., Kulstead, R.O.,more » McDuffee, C. and Schleicher, J.A., 1952, Oil Shale in Kansas, Kansas Geological Survey Bulletin, No. 96, Part 3.) five eastern Kansas black shale units were selected for study and their areal distribution mapped. The volume of recoverable oil shale in each unit was calculated and translated to reserves. The report concludes that in all probability, extraction of oil shale for shale oil is not feasible at this time due to the cost of extraction, transportation and processing. The report recommends that additional studies be undertaken to provide a more comprehensive and detailed assessment of Kansas oil shales as a potential fuel resource. 49 references, 4 tables.« less

  6. Rapid preconcentration method for the determination of azadirachtin-A and -B, nimbin and salannin in neem oil samples by using graphitised carbon solid phase extraction.

    PubMed

    Ramesh, A; Balasubramanian, M

    1999-01-01

    A simple and rapid method involving solid phase extraction and liquid chromatography for the determination of azadirachtin-A and -B, nimbin and salannin at nanogram levels in neem oil samples is presented. The neem oil samples are defatted and the compounds of interest extracted by mixing the sample with hexane and passing the hexane solution through a graphitised carbon black column. After washing the column with 2 ml of hexane, azadirachtin-A and -B, nimbin and salannin are eluted with 5 ml of acetonitrile and quantified using HPLC with UV detection. The recoveries of azadirachtin-A and -B, nimbin and salannin in fortified oil samples were 97.4-104.7%. The upper limit of quantification is up to 100 micrograms ml-1 without any additional clean-up and with little interference from lipids during the analysis by HPLC. The method was successfully applied to various neem oil samples collected from different locations in India.

  7. Decoloration Kinetics of Waste Cooking Oil by 60Co γ-ray/H2O2

    NASA Astrophysics Data System (ADS)

    Xiang, Yulin; Xiang, Yuxiu; Wang, Lipeng

    2016-03-01

    In order to decolorize, waste cooking oil, a dark red close to black solution from homes and restaurants, was subjected to 60Co γ-ray/H2O2 treatment. By virtue of UV/Vis spectrophotometric method, the influence of Gamma irradiation to decoloration kinetics and rate constants of the waste cooking oil in the presence of H2O2 was researched. In addition, the influence of different factors such as H2O2 concentration and irradiation dose on the decoloration rate of waste cooking oil was investigated. Results indicated that the decoloration kinetics of waste cooking oil conformed to the first-order reaction. The decoloration rate increased with the increase of irradiation dose and H2O2 concentration. Saponification analysis and sensory evaluation showed that the sample by 60Co γ-ray/H2O2 treatment presented better saponification performance and sensory score. Furthermore, according to cost estimate, the cost of the 60Co γ-ray/H2O2 was lower and more feasible than the H2O2 alone for decoloration of waste cooking oil.

  8. Mathematical modeling of polymer flooding using the unstructured Voronoi grid

    NASA Astrophysics Data System (ADS)

    Kireev, T. F.; Bulgakova, G. T.; Khatmullin, I. F.

    2017-12-01

    Effective recovery of unconventional oil reserves necessitates development of enhanced oil recovery techniques such as polymer flooding. The study investigated the model of polymer flooding with effects of adsorption and water salinity. The model takes into account six components that include elements of the classic black oil model. These components are polymer, salt, water, dead oil, dry gas and dissolved gas. Solution of the problem is obtained by finite volume method on unstructured Voronoi grid using fully implicit scheme and the Newton’s method. To compare several different grid configurations numerical simulation of polymer flooding is performed. The oil rates obtained by a hexagonal locally refined Voronoi grid are shown to be more accurate than the oil rates obtained by a rectangular grid with the same number of cells. The latter effect is caused by high solution accuracy near the wells due to the local grid refinement. Minimization of the grid orientation effect caused by the hexagonal pattern is also demonstrated. However, in the inter-well regions with large Voronoi cells flood front tends to flatten and the water breakthrough moment is smoothed.

  9. First ATRF Chili Contest Proves It’s Not Always about the Meat | Poster

    Cancer.gov

    Wafting cumin through the building, 15 slow cookers full of chili lined an L-shaped conference room at the Frederick National Laboratory for Cancer Research Advanced Technology Research Facility (ATRF), as voters strolled past “No electioneering” signs and the tasting began Feb. 29. Chili No. 13 came out of the gate with a spicy authority. “My tongue’s still tingling,” said

  10. JPRS Report, Near East and South Asia.

    DTIC Science & Technology

    1988-02-16

    now violating all laws and regulations here to enable us to send you the goods." Karl-Erik Schmitz has previously stressed to reporters that he has... cumin seeds, pumpkin seeds, coriander seeds, roasted pear seeds, turpentine trees, henna, dyes and indigo, gypsum, ready-made indoor and outdoor... stressing the impor- tance of local government as a nursery of national leaders. We should have learnt by now that non-party elections would

  11. Graphics Standards in the Computer-aided Acquisition and Logistic Support (CALS) Program, Fiscal Year 1989. Volume 1. Test Requirements Document and Extended CGM (CGEM)

    DTIC Science & Technology

    1990-07-01

    at the firet meng in Munich, it was foyud isthere exis•s some avedap between the stated goals of• • DG Standards and t•e CGt e SG con ded t in cumin ... stressing importance of liaison and harmonization with closely related groups; Japan - making suggestions that the NWI and Requirements Document be improved

  12. Assessment of undiscovered oil and gas resources of the Ordovician Utica Shale of the Appalachian Basin Province, 2012

    USGS Publications Warehouse

    Kirschbaum, Mark A.; Schenk, Christopher J.; Cook, Troy A.; Ryder, Robert T.; Charpentier, Ronald R.; Klett, Timothy R.; Gaswirth, Stephanie B.; Tennyson, Marilyn E.; Whidden, Katherine J.

    2012-01-01

    The U.S. Geological Survey assessed unconventional oil and gas resources of the Upper Ordovician Utica Shale and adjacent units in the Appalachian Basin Province. The assessment covers parts of Maryland, New York, Ohio, Pennsylvania, Virginia, and West Virginia. The geologic concept is that black shale of the Utica Shale and adjacent units generated hydrocarbons from Type II organic material in areas that are thermally mature for oil and gas. The source rocks generated petroleum that migrated into adjacent units, but also retained significant hydrocarbons within the matrix and adsorbed to organic matter of the shale. These are potentially technically recoverable resources that can be exploited by using horizontal drilling combined with hydraulic fracturing techniques.

  13. Thermal maturity and petroleum kitchen areas of Liassic Black Shales (Lower Jurassic) in the central Upper Rhine Graben, Germany

    NASA Astrophysics Data System (ADS)

    Böcker, Johannes; Littke, Ralf

    2016-03-01

    In the central Upper Rhine Graben (URG), several major oil fields have been sourced by Liassic Black Shales. In particular, the Posidonia Shale (Lias ɛ, Lower Toarcian) acts as excellent and most prominent source rock in the central URG. This study is the first comprehensive synthesis of Liassic maturity data in the URG area and SW Germany. The thermal maturity of the Liassic Black Shales has been analysed by vitrinite reflectance (VRr) measurements, which have been verified with T max and spore coloration index (SCI) data. In outcrops and shallow wells (<600 m), the Liassic Black Shales reached maturities equivalent to the very early or early oil window (ca. 0.50-0.60 % VRr). This maturity is found in Liassic outcrops and shallow wells in the entire URG area and surrounding Swabian Jura Mountains. Maximum temperatures of the Posidonia Shale before graben formation are in the order of 80-90 °C. These values were likely reached during Late Cretaceous times due to significant Upper Jurassic and minor Cretaceous deposition and influenced by higher heat flows of the beginning rift event at about 70 Ma. In this regard, the consistent regional maturity data (VRr, T max, SCI) of 0.5-0.6 % VRr for the Posidonia Shale close to surface suggest a major burial-controlled maturation before graben formation. These consistent maturity data for Liassic outcrops and shallow wells imply no significant oil generation and expulsion from the Posidonia Shale before formation of the URG. A detailed VRr map has been created using VRr values of 31 wells and outcrops with a structure map of the Posidonia Shale as reference map for a depth-dependent gridding operation. Highest maturity levels occur in the area of the Rastatt Trough (ca. 1.5 % VRr) and along the graben axis with partly very high VRr gradients (e.g. well Scheibenhardt 2). In these deep graben areas, the maximum temperatures which were reached during upper Oligocene to Miocene times greatly exceed those during the Cretaceous.

  14. 40 CFR 180.593 - Etoxazole; tolerances for residues.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... subgroup 9A 0.20 Milk, fat 0.01 Nut, tree, group 14 0.01 Papaya 0.20 Pepper/eggplant subgroup 8-10B 0.20..., black 0.20 Sapote, mamey 0.20 Sheep, fat 0.02 Sheep, liver 0.01 Spearmint, oil 20 Spearmint, tops 10...

  15. METHOD OF USING AND MANUFACTURING PLASTIC EQUIVALENT TO ORGANIC MATERIALS

    DOEpatents

    Shonka, F.R.; Rose, J.E.; Failla, G.

    1961-10-24

    Compositions of matter that have the radiation response of animal muscle tissue, bone, or air were prepared. These compositions are composed of specific proportions of three or more of the following constituents: polyethylene plastic, polyamide plastic, oil furnace black, silica, and calcium fluoride. (AEC)

  16. On the use of sodium lignosulphonate for enhanced oil recovery

    NASA Astrophysics Data System (ADS)

    Azis, M. M.; Rachmadi, H.; Wintoko, J.; Yuliansyah, A. T.; Hasokowati, W.; Purwono, S.; Rochmadi, W.; Murachman, B.

    2017-05-01

    There has been large interest to utilize oil reservoirs in Indonesia by using Enhanced Oil Recovery (EOR) processes. Injection of surfactant as a part of chemical injection technique in EOR is known to aid the mobility and reduction in surface tension. One potential surfactant for EOR application is Sodium Lignosulphonate (SLS) which can be made from various sources particularly empty fruit bunch of oil palm and black liquor from kraft pulp production. Here, we will discuss a number of methods for SLS production which includes lignin isolation techniques and sulphonation reaction. The use of SLS alone as EOR surfactant, however, is often not feasible as the Interfacial Tension (IFT) value of SLS is typically above the order of 10-3 dyne/cm which is mandated for EOR application. Hence, brief discussion on SLS formulation screening is provided which illustrates an extensive labwork experience during the SLS development in our lab.

  17. Al Jirab Island, United Arab Emirates, Persian Gulf

    NASA Image and Video Library

    1991-05-06

    STS039-71-088 (28 April-6 May 1991) --- A handheld 70mm frame of the southern Persian Gulf area. The island in the middle of the frame is Al Jirab, 30 miles west of Abu Dhabi in the United Arab Emirates. On the mainland beaches opposite the island, a thick and continuous black fringe of oil can be seen as a line. Photo experts studying the STS 39 photography have not yet ascertained the source of the oil. They disclosed that if the oil does in fact stem from damaged Kuwaiti offshore oil fields, the slick has been blown southward at least 460 miles. The city of Tarif is visible in the affected area. Lighter brown slicks can be seen offshore. A causeway joins Al Jirab to the mainland and a dredged ship channel with its associated islands can be seen west of the causeway.

  18. Sesamin and sesamolin as unexpected contaminants in various cold-pressed plant oils: NP-HPLC/FLD/DAD and RP-UPLC-ESI/MS(n) study.

    PubMed

    Górnaś, Paweł; Siger, Aleksander; Pugajeva, Iveta; Segliņa, Dalija

    2014-04-01

    Thirteen cold-pressed oils (Japanese quince seed, black caraway, flaxseed, rapeseed, hemp, peanut, sunflower, pumpkin, hazelnut, poppy, walnut, almond and sesame oil) manufactured by the same company over a 2-year period (2011-12) were assessed for lipophilic compounds. The presence of sesamin and sesamolin, two characteristic lignans of sesame oil, were detected in all tested plant oils. Both lignans were identified by NP-HPLC/FLD/DAD and confirmed by a RP-UPLC-ESI/MS(n) method. The lowest amount of sesamin and sesamolin was found for Japanese quince seed oil (0.10 and 0.27 mg/100 g), and the highest, excluding sesame oil, for almond oil (36.21 and 105.42 mg/100 g, respectively). The highly significant correlation between sesamolin and sesamin concentrations was found in all samples tested (r = 0.9999; p < 0.00001). These results indicate contamination of cold-pressed oils from the same source. This investigation highlights the fact that increasing the range of products manufactured by the same company can contribute to a lesser regard for the quality of the final product. Moreover, less attention paid to the quality of final product can be related to the health risks of consumers especially sensitive to allergens. Therefore, proper cleaning of processing equipment is needed to prevent cross-contact of cold-pressed oils.

  19. The Architecture and Frictional Properties of Faults in Shale

    NASA Astrophysics Data System (ADS)

    De Paola, N.; Imber, J.; Murray, R.; Holdsworth, R.

    2015-12-01

    The geometry of brittle fault zones in shale rocks, as well as their frictional properties at reservoir conditions, are still poorly understood. Nevertheless, these factors may control the very low recovery factors (25% for gas and 5% for oil) obtained during fracking operations. Extensional brittle fault zones (maximum displacement < 3 m) cut exhumed oil mature black shales in the Cleveland Basin (UK). Fault cores up to 50 cm wide accommodated most of the displacement, and are defined by a stair-step geometry. Their internal architecture is characterised by four distinct fault rock domains: foliated gouges; breccias; hydraulic breccias; and a slip zone up to 20 mm thick, composed of a fine-grained black gouge. Hydraulic breccias are located within dilational jogs with aperture of up to 20 cm. Brittle fracturing and cataclastic flow are the dominant deformation mechanisms in the fault core of shale faults. Velocity-step and slide-hold-slide experiments at sub-seismic slip rates (microns/s) were performed in a rotary shear apparatus under dry, water and brine-saturated conditions, for displacements of up to 46 cm. Both the protolith shale and the slip zone black gouge display shear localization, velocity strengthening behaviour and negative healing rates, suggesting that slow, stable sliding faulting should occur within the protolith rocks and slip zone gouges. Experiments at seismic speed (1.3 m/s), performed on the same materials under dry conditions, show that after initial friction values of 0.5-0.55, friction decreases to steady-state values of 0.1-0.15 within the first 10 mm of slip. Contrastingly, water/brine saturated gouge mixtures, exhibit almost instantaneous attainment of very low steady-state sliding friction (0.1), suggesting that seismic ruptures may efficiently propagate in the slip zone of fluid-saturated shale faults. Stable sliding in faults in shale can cause slow fault/fracture propagation, affecting the rate at which new fracture areas are created and, hence, limiting oil and gas production during reservoir stimulation. However, fluid saturated conditions can favour seismic slip propagation, with fast and efficient creation of new fracture areas. These processes are very effective at dilational jogs, where fluid circulation may be enhanced, facilitating oil and gas production.

  20. Dietary Plants for the Prevention and Management of Kidney Stones: Preclinical and Clinical Evidence and Molecular Mechanisms

    PubMed Central

    Nirumand, Mina Cheraghi; Hajialyani, Marziyeh; Rahimi, Roja; Farzaei, Mohammad Hosein; Nabavi, Seyed Mohammad

    2018-01-01

    Kidney stones are one of the oldest known and common diseases in the urinary tract system. Various human studies have suggested that diets with a higher intake of vegetables and fruits play a role in the prevention of kidney stones. In this review, we have provided an overview of these dietary plants, their main chemical constituents, and their possible mechanisms of action. Camellia sinensis (green tea), Rubus idaeus (raspberry), Rubia cordifolia (common madder), Petroselinum crispum (parsley), Punica granatum (pomegranate), Pistacia lentiscus (mastic), Solanum xanthocarpum (yellow-fruit nightshade), Urtica dioica (stinging nettle), Dolichos biflorus (horse gram), Ammi visnaga (khella), Nigella sativa (black-cumin), Hibiscus sabdariffa (roselle), and Origanum vulgare (oregano) have received considerable interest based on scientific evidence. Beside these dietary plants, phytochemicals—such as catechin, epicatechin, epigallocatechin-3-gallate, diosmin, rutin, quercetin, hyperoside, and curcumin—as antioxidant dietary phyto-phenols were found to be effective for the prevention of urolithiasis (the process of stone formation in the urinary tract). The main underlying mechanisms of these dietary plants and their isolated phytonutrients in the management of urolithiasis include diuretic, antispasmodic, and antioxidant activity, as well as an inhibitory effect on crystallization, nucleation, and aggregation of crystals. The results as presented in this review demonstrate the promising role of dietary plants and phytophenols in the prevention and management of kidney stones. Further investigations are required to confirm the safety and efficacy of these compounds. PMID:29518971

  1. Dietary Plants for the Prevention and Management of Kidney Stones: Preclinical and Clinical Evidence and Molecular Mechanisms.

    PubMed

    Nirumand, Mina Cheraghi; Hajialyani, Marziyeh; Rahimi, Roja; Farzaei, Mohammad Hosein; Zingue, Stéphane; Nabavi, Seyed Mohammad; Bishayee, Anupam

    2018-03-07

    Kidney stones are one of the oldest known and common diseases in the urinary tract system. Various human studies have suggested that diets with a higher intake of vegetables and fruits play a role in the prevention of kidney stones. In this review, we have provided an overview of these dietary plants, their main chemical constituents, and their possible mechanisms of action. Camellia sinensis (green tea), Rubus idaeus (raspberry), Rubia cordifolia (common madder), Petroselinum crispum (parsley), Punica granatum (pomegranate), Pistacia lentiscus (mastic), Solanum xanthocarpum (yellow-fruit nightshade), Urtica dioica (stinging nettle), Dolichos biflorus ( horse gram ), Ammi visnaga (khella), Nigella sativa (black-cumin), Hibiscus sabdariffa (roselle), and Origanum vulgare (oregano) have received considerable interest based on scientific evidence. Beside these dietary plants, phytochemicals-such as catechin, epicatechin, epigallocatechin-3-gallate, diosmin, rutin, quercetin, hyperoside, and curcumin-as antioxidant dietary phyto-phenols were found to be effective for the prevention of urolithiasis (the process of stone formation in the urinary tract). The main underlying mechanisms of these dietary plants and their isolated phytonutrients in the management of urolithiasis include diuretic, antispasmodic, and antioxidant activity, as well as an inhibitory effect on crystallization, nucleation, and aggregation of crystals. The results as presented in this review demonstrate the promising role of dietary plants and phytophenols in the prevention and management of kidney stones. Further investigations are required to confirm the safety and efficacy of these compounds.

  2. The comparison of anticancer activity of thymoquinone and nanothymoquinone on human breast adenocarcinoma.

    PubMed

    Dehghani, Hossein; Hashemi, Mehrdad; Entezari, Maliheh; Mohsenifar, Afshin

    2015-01-01

    Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. The identification of new cytotoxic drugs with low side effects on immune system has developed as important area in new studies of pharmacology. Thymoquinone (TQ), derived from the medicinal spice Nigella sativa (also calledt black cumin) exhibit anti-inflammatory and anti-cancer activities. In this study we employed nanogel-based nanoparticle approach to improve upon its effectiveness. Myristic acid-chitosan (MA-chitosan) nanogels were prepared by the technique of self-assembly. Thymoquinone was loaded into the nanogels. The surface morphology of the prepared nanoparticles was determined using SEM and TEM. The other objective of this study was to examine the in-vitro cytotoxic activity of cell death of Thymoquinone and nanothymoquinone on human breast adenocarcinoma cell line (MCF7). Cytotoxicity and viability of Thymoquinone and nanothymoquinone were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Transmission electron microscopy confirmed the particle diameter was between 150 to 200 nm. Proliferation of MCF7 cells was significantly inhibited by Thymoquinone and nanothymoquinone in a concentration-dependent manner in defined times. There were significant differences in IC50 Thymoquinone and nanothymoquinone. TQ-loaded nanoparticles proved more effective compared to TQ solution. The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles.

  3. Thymoquinone Suppresses IRF-3-Mediated Expression of Type I Interferons via Suppression of TBK1

    PubMed Central

    Cho, Jae Youl

    2018-01-01

    Interferon regulatory factor (IRF)-3 is known to have a critical role in viral and bacterial innate immune responses by regulating the production of type I interferon (IFN). Thymoquinone (TQ) is a compound derived from black cumin (Nigella sativa L.) and is known to regulate immune responses by affecting transcription factors associated with inflammation, including nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). However, the role of TQ in the IRF-3 signaling pathway has not been elucidated. In this study, we explored the molecular mechanism of TQ-dependent regulation of enzymes in IRF-3 signaling pathways using the lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cell line. TQ decreased mRNA expression of the interferon genes IFN-α and IFN-β in these cells. This inhibition was due to its suppression of the transcriptional activation of IRF-3, as shown by inhibition of IRF-3 PRD (III-I) luciferase activity as well as the phosphorylation pattern of IRF-3 in the immunoblotting experiment. Moreover, TQ targeted the autophosphorylation of TANK-binding kinase 1 (TBK1), an upstream key enzyme responsible for IRF-3 activation. Taken together, these findings suggest that TQ can downregulate IRF-3 activation via inhibition of TBK1, which would subsequently decrease the production of type I IFN. TQ also regulated IRF-3, one of the inflammatory transcription factors, providing a novel insight into its anti-inflammatory activities. PMID:29751576

  4. The Comparison of Anticancer Activity of Thymoquinone and Nanothymoquinone on Human Breast Adenocarcinoma

    PubMed Central

    Dehghani, Hossein; Hashemi, Mehrdad; Entezari, Maliheh; Mohsenifar, Afshin

    2015-01-01

    Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. The identification of new cytotoxic drugs with low side effects on immune system has developed as important area in new studies of pharmacology. Thymoquinone (TQ), derived from the medicinal spice Nigella sativa (also calledt black cumin) exhibit anti-inflammatory and anti-cancer activities. In this study we employed nanogel-based nanoparticle approach to improve upon its effectiveness. Myristic acid-chitosan (MA-chitosan) nanogels were prepared by the technique of self-assembly. Thymoquinone was loaded into the nanogels. The surface morphology of the prepared nanoparticles was determined using SEM and TEM. The other objective of this study was to examine the in-vitro cytotoxic activity of cell death of Thymoquinone and nanothymoquinone on human breast adenocarcinoma cell line (MCF7). Cytotoxicity and viability of Thymoquinone and nanothymoquinone were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Transmission electron microscopy confirmed the particle diameter was between 150 to 200 nm. Proliferation of MCF7 cells was significantly inhibited by Thymoquinone and nanothymoquinone in a concentration-dependent manner in defined times. There were significant differences in IC50 Thymoquinone and nanothymoquinone. TQ-loaded nanoparticles proved more effective compared to TQ solution. The high drug-targeting potential and efficiency demonstrates the significant role of the anticancer properties of TQ-loaded nanoparticles. PMID:25901162

  5. Adjustable liquid aperture to eliminate undesirable light in holographic projection.

    PubMed

    Wang, Di; Liu, Chao; Li, Lei; Zhou, Xin; Wang, Qiong-Hua

    2016-02-08

    In this paper, we propose an adjustable liquid aperture to eliminate the undesirable light in a holographic projection. The aperture is based on hydrodynamic actuation. A chamber is formed with a cylindrical tube. A black droplet is filled in the sidewall of the cylinder tube and the outside space is the transparent oil which is immiscible with the black droplet. An ultrathin glass sheet is attached on the bottom substrate of the device and a black shading film is secured to the central area of the glass sheet. By changing the volume of the black droplet, the black droplet will move to the middle or sidewall due to hydrodynamic actuation, so the device can be used as an adjustable aperture. A divergent spherical wave and a solid lens are used to separate the focus planes of the reconstructed image and diffraction beams induced by the liquid crystal on silicon in the holographic projection. Then the aperture is used to eliminate the diffraction beams by adjusting the size of the liquid aperture and the holographic projection does not have undesirable light.

  6. The Other Major 2010 Oil Spill: Oil weathering after the Kalamazoo River Dilbit Spill

    NASA Astrophysics Data System (ADS)

    Swarthout, B.; Reddy, C. M.; Nelson, R. K.; Hamilton, S. K.; Aeppli, C.; Valentine, D. L.; Fundaun, S. E.; Oliveira, A. H.

    2016-02-01

    Diluted bitumen (dilbit) from the oil sands (tar sands) of western Canada is increasingly being transported to US markets. North America's largest inland oil spill and the first major oil sands spill in a freshwater environment occurred in 2010, when at least 843,000 gallons leaked from a pipeline into the Kalamazoo River of southwest Michigan. Cleanup of this oil was unusually difficult and protracted, lasting through 2014 and costing over a billion dollars, largely because a substantial fraction of the oil became submersed and deposited in slack water areas over 60 km of river channel, reservoirs, and floodplain backwaters. To investigate the fate of the spilled dilbit from the 2010 Kalamazoo River release, black rings, presumably oil residues, on the bark of dead trees were collected in 2015. These residues were deposited on the trees during high flood levels that have not been observed since the spill and represent an opportunity to constrain weathering processes excluding dissolution. This material contained a major non-GC amenable fraction of 90-95%, presumably oxygenated hydrocarbons. The GC amenable portion was consistent with laboratory weathered dilbit. We used a variety of analytical tools to characterize the dilbit residues, as well as to identify dilbit weathering processes that occurred since the spill.

  7. Efficacy improvement of a bioherbicidal fungus using a formulation-based approach

    USDA-ARS?s Scientific Manuscript database

    Greenhouse experiments were conducted to determine the effects of an invert (water - in - oil) emulsion (IE) on dew period duration and dew delay of Colletotrichum coccodes for biocontrol of the problematic weed, eastern black nightshade (Solanum ptycanthum). Dew periods of 4, 8, or 12 h provided ...

  8. Fuels from Recycling Systems

    ERIC Educational Resources Information Center

    Tillman, David A.

    1975-01-01

    Three systems, operating at sufficient scale, produce fuels that may be alternatives to oil and gas. These three recycling systems are: Black Clawson Fiberclaim, Franklin, Ohio; Union Carbide, South Charleston, West Virginia; and Union Electric, St. Louis, Missouri. These produce a wet fuel, a pyrolytic gas, and a dry fuel, respectively. (BT)

  9. Irresistible

    ERIC Educational Resources Information Center

    Curio, Michele

    2005-01-01

    One of Michele Curio's favorite art lessons is creating a resist using oil pastels under black tempera paint. The process produces dramatic and creative results with a high success rate even for the most art-challenged students. The artworks have a sophisticated, painterly quality that is achieved with more control and less mess than direct…

  10. 40 CFR 428.71 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Fabricated Rubber Plants Subcategory § 428.71 Specialized definitions. For the purpose of this subpart: (a... natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in the manufacture... divided by the total volume of air scrubbed. (d) The term “large-sized plants” shall mean plants which...

  11. 40 CFR 428.71 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.71 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “large-sized plants” shall...

  12. 40 CFR 428.51 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Fabricated Rubber Plants Subcategory § 428.51 Specialized definitions. For the purpose of this subpart: (a... natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in the manufacture... divided by the total volume of air scrubbed. (d) The term “small-sized plants” shall mean plants which...

  13. 40 CFR 428.51 - Specialized definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.51 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “small-sized plants” shall...

  14. 40 CFR 428.51 - Specialized definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Fabricated Rubber Plants Subcategory § 428.51 Specialized definitions. For the purpose of this subpart: (a... natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in the manufacture... divided by the total volume of air scrubbed. (d) The term “small-sized plants” shall mean plants which...

  15. 40 CFR 428.61 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Fabricated Rubber Plants Subcategory § 428.61 Specialized definitions. For the purpose of this subpart: (a... natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in the manufacture... divided by the total volume of air scrubbed. (d) The term “medium-sized plants” shall mean plants which...

  16. 40 CFR 428.51 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.51 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “small-sized plants” shall...

  17. 40 CFR 428.61 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.61 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “medium-sized plants” shall...

  18. 40 CFR 428.51 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.51 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “small-sized plants” shall...

  19. 40 CFR 428.61 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.61 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “medium-sized plants” shall...

  20. 40 CFR 428.71 - Specialized definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.71 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “large-sized plants” shall...

  1. 40 CFR 428.71 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.71 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “large-sized plants” shall...

  2. 40 CFR 428.61 - Specialized definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Extruded, and Fabricated Rubber Plants Subcategory § 428.61 Specialized definitions. For the purpose of...” shall mean all natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in... wet scrubbers divided by the total volume of air scrubbed. (d) The term “medium-sized plants” shall...

  3. 40 CFR 428.61 - Specialized definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Fabricated Rubber Plants Subcategory § 428.61 Specialized definitions. For the purpose of this subpart: (a... natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in the manufacture... divided by the total volume of air scrubbed. (d) The term “medium-sized plants” shall mean plants which...

  4. 40 CFR 428.71 - Specialized definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Fabricated Rubber Plants Subcategory § 428.71 Specialized definitions. For the purpose of this subpart: (a... natural and synthetic rubber, carbon black, oils, chemical compounds, and fabric used in the manufacture... divided by the total volume of air scrubbed. (d) The term “large-sized plants” shall mean plants which...

  5. Little River Inlet Navigation Project, Brunswick County, North Carolina and Horry County, South Carolina. Revision.

    DTIC Science & Technology

    1977-06-01

    to gr(ater physiological stress than the Adjacent waterways. In addition to the nine stations in I1,, inner channel, eight others were occupied in...rmn’, u t I,,’ i on. U. S. G, t. Print in Offic , Washington, D.C. 3 . . Cumin rc Nat i una I i i c i\\ tiospher i c Adri ii st , r , . ) 0 . Tidt d , i

  6. Great III - Ecological and Habitat Characterization. Primary Document.

    DTIC Science & Technology

    1982-05-01

    and Cumins , 1978 2ilsesoff, 1975 Holsinger, 1972 1168e, A .. 1976 Schuster and Etnier, 1978 Lippeom and Moran, 1974 Mackie et al., 1960 Mansueti ad... stresses . The reverse is indicated by low diversity and evenness values. For the benthos, two types of diversity and evenness values were calculated. Because...tolerant of environmental stress and often proliferate in areas receiving heavy organic inputs (bewage, for example). L Uruina 3~t reuatl found- in shallow

  7. Comparing the greenhouse gas emissions from three alternative waste combustion concepts

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Vainikka, Pasi, E-mail: pasi.vainikka@vtt.fi; Tsupari, Eemeli; Sipilae, Kai

    2012-03-15

    Highlights: Black-Right-Pointing-Pointer Significant GHG reductions are possible by efficient WtE technologies. Black-Right-Pointing-Pointer CHP and high power-to-heat ratio provide significant GHG savings. Black-Right-Pointing-Pointer N{sub 2}O and coal mine type are important in LCA GHG emissions of FBC co-combustion. Black-Right-Pointing-Pointer Substituting coal and fuel oil by waste is beneficial in electricity and heat production. Black-Right-Pointing-Pointer Substituting natural gas by waste may not be reasonable in CHP generation. - Abstract: Three alternative condensing mode power and combined heat and power (CHP) waste-to-energy concepts were compared in terms of their impacts on the greenhouse gas (GHG) emissions from a heat and power generation system.more » The concepts included (i) grate, (ii) bubbling fluidised bed (BFB) and (iii) circulating fluidised bed (CFB) combustion of waste. The BFB and CFB take advantage of advanced combustion technology which enabled them to reach electric efficiency up to 35% and 41% in condensing mode, respectively, whereas 28% (based on the lower heating value) was applied for the grate fired unit. A simple energy system model was applied in calculating the GHG emissions in different scenarios where coal or natural gas was substituted in power generation and mix of fuel oil and natural gas in heat generation by waste combustion. Landfilling and waste transportation were not considered in the model. GHG emissions were reduced significantly in all of the considered scenarios where the waste combustion concepts substituted coal based power generation. With the exception of condensing mode grate incinerator the different waste combustion scenarios resulted approximately in 1 Mton of fossil CO{sub 2}-eq. emission reduction per 1 Mton of municipal solid waste (MSW) incinerated. When natural gas based power generation was substituted by electricity from the waste combustion significant GHG emission reductions were not achieved.« less

  8. Current issues in natural gas lubrication

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Reber, J.

    1997-10-01

    Because of the ability of natural gas to burn completely relatively easily, supplying excess oxygen to promote complete reactions is a viable alternative to catalysts. Hence, lean burn technology has a natural fit for this industry. Lube oil is not adversely affected by lean burn operation. There is a slight tendency to cause more oil nitration than oxidation, but the real difference is not significant. Operators may notice somewhat more varnish (caramel color) and less sludge (black) as a result. Because the fuel is burned more completely, there is less problem with fuel-derived oil contamination. Also because of the excessmore » air in the combustion chamber, overall cylinder temperature is lower, causing less stress on the oil. Oil life is generally lengthened. One common misconception that lean burn engines require different lubricants may stem from a change at Waukesha Engine Division--Dresser Industries. Waukesha has changed its lube oil requirements for VHP 3521, 5115, 7042, 9390 GL turbocharged and lean burn model engines. The lube oil specification for these engines is 1% to 1.7% ash with the same 0.10% zinc maximum. This change is not because of the lean burn nature of these engines, rather it is because of drastically decreased lube oil consumption. With less oil consumption, less ash is carried to the critical exhaust valve seat area to prevent valve recession.« less

  9. Cosmetic Cleansing Oil Absorption by Soft Contact Lenses in Dry and Wet Conditions.

    PubMed

    Tsukiyama, Junko; Miyamoto, Yuko; Kodama, Aya; Fukuda, Masahiko; Shimomura, Yoshikazu

    2017-09-01

    Previous reports showed that cosmetic cleansing oil for removing makeup, which contains mineral oil and surfactant, can deform some silicone hydrogel contact lenses (SHCLs) when applied directly to the lenses, although plasma-coated SHCLs (lotrafilcon A and B) were not affected. In the present study, we investigated hydrogel lenses and SHCLs in both wet and dry conditions. Several brands of hydrogel and SHCLs were immersed in a cleansing oil solution containing Sudan Black B for 5 min under wet and dry conditions. The lenses under the wet condition were simply picked up from the saline, whereas those under the dry condition were blotted with paper wipes. After immersing, the excess solution remaining on the lenses was removed by finger rubbing with a multipurpose solution. The lenses were then examined using a stereomicroscope, and their mean brightness was measured and compared. The cosmetic cleansing oil was not absorbed by the hydrogel lenses under wet or dry conditions. However, four of seven brands of SHCLs absorbed the cosmetic cleansing oil under both conditions (dry and wet), whereas asmofilcon A absorbed it only under the dry condition. Lotrafilcon B and delefilcon A did not absorb cleansing oil even under the dry condition. Hydrogel lenses resist cosmetic cleansing oil. However, SHCLs have different degrees of resistance depending on the lens material. Some SHCLs absorbed cosmetic cleansing oil more under dry conditions than under wet conditions.

  10. Gynecology: select topics.

    PubMed

    Sidani, Mohamad; Campbell, James

    2002-06-01

    Menopause, premenstrual syndrome, dysmenorrhea, female fertility, and mastalgia are common problems not easily treated by conventional medicine. Women often seek alternative therapies to help address these conditions. Some evidence points to the efficacy of black cohosh, exercise, and possibly Kava and St. John's wort, in the treatment of menopausal symptoms. Clinical trials indicate that symptoms of premenstrual syndrome may be alleviated with calcium, magnesium, vitamin E. Thiamine, omega-3 fatty acids, the Japanese herbal concoction, TSS, and calcium have proved useful in treating women with dysmenorrhea. Symptoms of mastalgia may be attenuated by evening primrose oil, chaste tree and flaxseed oil may be helpful.

  11. Egg Products for Use in a Cook-Freeze System

    DTIC Science & Technology

    1976-08-01

    ounces) of water„ Peppers, green, finely chopped 4.56 1,65 748.4 3. Saute onions Oil, vegetable 1.98 0.72 326.6 and peppers in oil Butter 1^9 8 2...chestnuts, canned 6.84 2.48 1,124.9 5. Blend egg chopped white/methocel, Soy sauce 1.82 0.66 299.4 sauteed onions and Monosodium glutamate 0.05 0.02 9.1...black pepper, smoke Egg, yolk, blended 30.68 11.11 5,039.5 salt, nonfat dry 12 «PMP Ingredients Intage Pounds Grama Procedure Ham, chopped 15.21

  12. Polychlorinated Biphenyls in the Plasma and Preen Oil of Black-Footed Albatross (Diomedea nigripes) Chicks and Adults on Midway Atoll, North Pacific Ocean.

    PubMed

    Wang, Jun; Caccamise, Sarah A L; Woodward, Lee Ann; Li, Qing X

    2015-01-01

    Polychlorinated biphenyls (PCBs) are ubiquitous in the environment. Midway Atoll, located in the North Pacific Ocean, was occupied by the military during and after World War II. However, Midway Atoll has become a national wildlife refuge and home to many different seabirds today, including the black-footed albatross (Diomedea nigripes) (BFAL). The profiles and toxic equivalents (TEQ) of PCB congeners in the plasma and preen oil of BFAL chicks and adults were determined in this study. The concentrations of the total PCBs in the plasma samples of chicks and adults collected in Midway Atoll ranged from 2.3 to 223.8 (mean 80.1) and 22.8 to 504.5 (mean 158.6) ng g(-1) (wet weight, ww), respectively. The TEQs ranged from 0.2 to 0.6 (mean 0.4) and 0.4 to 1.6 (mean 0.9) pg g(-1) ww, respectively, in the plasma samples of chicks and adults from Midway Atoll. The major congeners in the plasma samples of chicks and adults included PCBs 31, 87, 97, 99, 118, 138, 153, and 180, accounting for 70% of the total PCBs. The concentrations of the total PCBs in the adult preen oil samples ranged from 1693 to 39404 (mean 10122) ng g(-1) (ww), of which 97% were PCBs 105, 118, 128, 138, 153, 161, 172, and 183.

  13. Self-dispersed crumpled graphene balls in oil for friction and wear reduction

    PubMed Central

    Dou, Xuan; Koltonow, Andrew R.; He, Xingliang; Jang, Hee Dong; Wang, Qian; Chung, Yip-Wah; Huang, Jiaxing

    2016-01-01

    Ultrafine particles are often used as lubricant additives because they are capable of entering tribological contacts to reduce friction and protect surfaces from wear. They tend to be more stable than molecular additives under high thermal and mechanical stresses during rubbing. It is highly desirable for these particles to remain well dispersed in oil without relying on molecular ligands. Borrowing from the analogy that pieces of paper that are crumpled do not readily stick to each other (unlike flat sheets), we expect that ultrafine particles resembling miniaturized crumpled paper balls should self-disperse in oil and could act like nanoscale ball bearings to reduce friction and wear. Here we report the use of crumpled graphene balls as a high-performance additive that can significantly improve the lubrication properties of polyalphaolefin base oil. The tribological performance of crumpled graphene balls is only weakly dependent on their concentration in oil and readily exceeds that of other carbon additives such as graphite, reduced graphene oxide, and carbon black. Notably, polyalphaolefin base oil with only 0.01–0.1 wt % of crumpled graphene balls outperforms a fully formulated commercial lubricant in terms of friction and wear reduction. PMID:26811466

  14. Screening, nutritional optimization and purification for phytase produced by Enterobacter aerogenes and its role in enhancement of hydrocarbons degradation and biofilm inhibition.

    PubMed

    Muslim, Sahira Nsayef; Mohammed Ali, Alaa Naseer; Al-Kadmy, Israa M S; Khazaal, Saba Saadoon; Ibrahim, Susan A; Al-Saryi, Nadal A; Al-Saadi, Luma Ghaeb; Muslim, Sraa Nsayef; Salman, Batool Kadham; Aziz, Sarah Naji

    2018-02-01

    In this study, a novel isolate of Enterobacter aerogenes isolated from contaminated soils with hydrocarbons had extracellular phytate-degrading activity. Enterobacter aerogenes isolates were identified by biochemical tests and confirmed by16S rRNA gene products (amplified size 211bp) for genotypic detection. The phytase activity was reached to maximum activity when this isolate was cultivated under the optimal conditions which consisted of using minimal salt medium containing 1%(w/v) rice bran as a sole source for carbon and 2% (w/v) yeast extract at pH 5.5 and temperature of 50°C for 48 h. The phytase had purified to homogeneity by 50% ammonium sulphate precipitation, ion exchange and gel filtration chromatography with 75.7 fold of purification and a yield of 30.35%. The purified phytase is a single peptide with approximate molecular mass of 42 kDa as assessed by SDS-PAGE. The highest degradative ability by Enterobacter aerogenes of black oil, white oil and used engine oil had observed after 72 h of incubation. Rapid degradation of black oil and used engine oil had also observed while slow degradation of white oilat all time of incubation. The purified phytase inhibited biofilm formation ability in a dose-dependent manner for all Gram-negative and Gram-positive biofilm-forming bacteria and a significant difference in cell surface hydrophobicity was observed after exposure of planktonic cells to phytase for hour. The hydrolyzing effect of phytase released by Enterobacter aerogenes for complex salts of phosphorus that are insoluble in the soil led to increase of phosphorus concentrations and enhanced the ability of Enterobacter aerogenes to degrade a specific hydrocarbon in contaminated soil so that the phytase has a promising application in bioremediation of contaminated soils with hydrocarbons. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. 24 CFR 3280.703 - Minimum standards.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Standard Specification for Pipe, Steel, Black and Hot-Dipped, Zinc-Coated, Welded and Seamless—ASTM A53-93. Standard Specification for Electric-Resistance-Welded Coiled Steel Tubing for Gas and Fuel Oil Lines—ASTM A539-1999. Pipe Threads, General Purpose (Inch)—ANSI/ASME B1.20.1-1983. Welding and Seamless Wrought...

  16. Toxicity and repellency of plant essential oils against the arthropod disease vectors Phlebotomus papatasi and Ixodes scapularis

    USDA-ARS?s Scientific Manuscript database

    The sand fly Phlebotomus papatasi is an important blood feeder and the main vector of the trypanosomatid protozoa Leishmania major, which causes leishmaniasis in parts of the Afro-Eurasian region. The black- legged tick Ixodes scapularis is the primary tick vector of the bacterium Borrelia burgdorfe...

  17. Laboratory plate tectonics: a new experiment.

    PubMed

    Gans, R F

    1976-03-26

    A "continent" made of a layer of hexagonally packed black polyethylene spheres floating in clear silicon oil breaks into subcontinents when illuminated by an ordinary incandescent light bulb. This experiment may be a useful model of plate tectonics driven by horizontal temperature gradients. Measurements of the spreading rate are made to establish the feasibility of this model.

  18. USSR Report, Energy.

    DTIC Science & Technology

    1983-06-03

    Agriculture Construction and Related Industries Consumer Goods and Domestic Trade Economic Affairs Energy Human Resources International Economic...Relations Transportation Physics and Mathmetics Space Space Biology and Aerospace Medicine Military Affairs Chemistry Cybernetics, Computers...tons of "black , it is necessary to mention the n enormous contribution to the atar ASSR’s oil industry. The iation /Tatar ASSR Production

  19. Effect of black pepper essential oil on the quality of fresh pork during storage.

    PubMed

    Zhang, Jing; Wang, Ying; Pan, Dao-Dong; Cao, Jin-Xuan; Shao, Xing-Feng; Chen, Yin-Ji; Sun, Yang-Ying; Ou, Chang-Rong

    2016-07-01

    The effect of different concentrations (0, 0.1 and 0.5%, v/v) of black pepper essential oil (BPEO) on thiobarbituric acid reactive substances (TBARS), meat color, the percentage of metmyoglobin (MetMb%), microbiological parameters and total volatile basic nitrogen (TVB-N) of pork loins stored at 4°C for 9days was evaluated. BPEO treatments showed lower TBARS, MetMb%, yellowness (b*) values, Pseudomonas spp. and Enterobacteriaceae count and TVB-N values and higher lightness (L*) and redness (a*) values than the control during storage; the effectiveness of BPEO was dose-dependent. The retardation of the formation of MetMb by adding BPEO ensured higher L* and a* values and lower b* values than the control at 6 and 9days; the MetMb content has a similar trend to the lipid oxidation. The lower TVB-N value of BPEO treatments than the control could be attributed to the inhibition of Pseudomonas spp. and Enterobacteriaceae. Gram-negative bacteria were more sensitive than Gram-positive bacteria to BPEO. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. The use of linear expressions of solute boiling point versus retention to indicate special interactions with the molecular rings of modified cyclodextrin phases in gas chromatography

    PubMed

    Betts

    2000-08-01

    The boiling points (degrees C, 1 x 10) of diverse C10 polar solutes from volatile oils are set against their relative retention times versus n-undecane to calculate linear equations for 12 commercial modified cyclodextrin (CD) capillary phases. Ten data points are considered for each CD, then solutes are rejected until 5 or more remain that give an expression with a correlation coefficient of at least 0.990 and a standard deviation of less than 5.5. Three phases give almost perfect correlation, and 3 other CDs have difficulty complying. Solutes involved in the equations (most frequently cuminal, linalol, and carvone) are presumed to have a 'standard' polar transient interaction with the molecular rings of the CDs concerned. Several remaining solutes (mostly citral, fenchone, and menthol) exhibit extra retention over the calculated standard (up to 772%), which is believed to indicate a firm 'host' CD or 'guest' solute molecular fit in some cases. Other solutes show less retention than calculated (mostly citronellal, citronellol, estragole, and pulegone). This suggests rejection by the CD, which behaves merely as a conventional stationary phase to them. The intercept constant in the equation for each phase is suggested to be a numerical relative polarity indicator. These b values indicate that 3 hydroxypropyl CDs show the most polarity with values from 28 to 43; and CDs that are fully substituted with inert groups fall in the range of 15 to 20.

  1. Black shale source rocks and oil generation in the Cambrian and Ordovician of the central Appalachian Basin, USA

    USGS Publications Warehouse

    Ryder, R.T.; Burruss, R.C.; Hatch, J.R.

    1998-01-01

    Nearly 600 million bbl of oil (MMBO) and 1 to 1.5 trillion ft3 (tcf) of gas have been produced from Cambrian and Ordovician reservoirs (carbonate and sandstone) in the Ohio part of the Appalachian basin and on adjoining arches in Ohio, Indiana, and Ontario, Canada. Most of the oil and gas is concentrated in the giant Lima-Indiana field on the Findlay and Kankakee arches and in small fields distributed along the Knox unconformity. Based on new geochemical analyses of oils, potential source rocks, bitumen extracts, and previously published geochemical data, we conclude that the oils in both groups of fields originated from Middle and Upper Ordovician blcak shale (Utica and Antes shales) in the Appalachian basin. Moroever, we suggest that approximately 300 MMBO and many trillions of cubic feet of gas in the Lower Silurian Clinton sands of eastern Ohio originated in the same source rocks. Oils from the Cambrian and Ordovician reservoirs have similar saturated hydrocarbon compositions, biomarker distributions, and carbon isotope signatures. Regional variations in the oils are attributed to differences in thermal maturation rather than to differences in source. Total organic carbon content, genetic potential, regional extent, and bitument extract geochemistry identify the balck shale of the Utica and Antes shales as the most plausible source of the oils. Other Cambrian and Ordovician shale and carbonate units, such as the Wells Creek formation, which rests on the Knox unconformity, and the Rome Formation and Conasauga Group in the Rome trough, are considered to be only local petroleum sources. Tmax, CAI, and pyrolysis yields from drill-hole cuttings and core indicate that the Utica Shale in eastern and central Ohio is mature with respect to oil generation. Burial, thermal, and hydrocarbon-generation history models suggest that much of the oil was generated from the Utica-Antes source in the late Paleozoic during the Alleghanian orogeny. A pervasive fracture network controlled by basement tectonics aided in the distribution of oil from the source to the trap. This fracture network permitted oil to move laterally and stratigraphically downsection through eastward-dipping, impermeable carbonate sequences to carrier zones such as the Middle Ordovician Knox unconformity, and to reservoirs such as porous dolomite in the Middle Ordovician Trenton Limestone in the Lima-Indiana field. Some of the oil and gas from the Utica-Antes source escaped vertically through a partially fractured, leaky Upper Ordovician shale seal into widespread Lower Silurian sandstone reservoirs.Nearly 600 million bbl of oil (MMBO) and 1 to 1.5 trillion ft3 (tcf) of gas have been produced from Cambrian and Ordovician reservoirs (carbonate and sandstone) in the Ohio part of the Appalachian basin and on adjoining arches in Ohio, Indiana, and Ontario, Canada. Most of the oil and gas is concentrated in the giant Lima-Indiana field on the Findlay and Kankakee arches and in small fields distributed along the Knox unconformity. Based on new geochemical analyses of oils, potential source rocks, bitumen extracts, and previously published geochemical data, we conclude that the oils in both groups of fields originated from Middle and Upper Ordovician black shale (Utica and Antes shales) in the Appalachian basin. Moreover, we suggest that approximately 300 MMBO and many trillions of cubic feet of gas in the Lower Silurian Clinton sands of eastern Ohio originated in these same source rocks.

  2. Twin Valley, Wild Rice River, Minnesota. Addendum.

    DTIC Science & Technology

    1976-05-01

    acres each and one having a drainage area of over 13,000 acres. Along with the impoundment program, p!-oper management of soil and water is stressed ...diversity of both plants and animals associated with this ecotone and stresses its importance: first, because it is an ecotone, and second, because it...1969. Pole stage aspen woods. Thirty-third breeding bird census. Aud. Field Notes 23: 712-713. Cumins , K.W. 1962. An evaluation of some techniques for

  3. Grays Harbor and Chehalis River Improvements to Navigation Environmental Studies. Wildlife Studies at Proposed Disposal Sites in Grays Harbor, Washington,

    DTIC Science & Technology

    1982-01-01

    sltand. T 𔃼~P i’ W 210 three times VtwCerI November IOC’C -nd ~co l.Etls ~ ec!,!zervc-o betxwe H -gF 12 Th -ind hl rway u- 7Plie Sicuobh. E. Cumin -s 1... stress imposed by dredge dsosal ;ictivities on these species. It is difficult to rredict the effects of establishing a salt marsh in Grays Harbor on

  4. JPRS Report, Near East & South Asia.

    DTIC Science & Technology

    1987-08-11

    political situation was better than it had been in 1967, but 84 percent of the answers stressed that the Arab summit conferences had not succeeded in...34 Rabin stressed that the police was following the law in dealing with the suspects arrested in the riots, and that he was convinced that the report he...no exporting and do not understand it. None of those things we see as exportable traditional goods, such as carpets, cumin and so forth, were part of

  5. IMAA (Integrated Measurements of Aerosol in Agri valley) campaign: Multi-instrumental observations at the largest European oil/gas pre-treatment plant area

    NASA Astrophysics Data System (ADS)

    Calvello, Mariarosaria; Caggiano, Rosa; Esposito, Francesco; Lettino, Antonio; Sabia, Serena; Summa, Vito; Pavese, Giulia

    2017-11-01

    A short-term intensive multi-instrumental measurement campaign (Integrated Measurements of Aerosol in Agri valley - IMAA) was carried out near the largest European oil and gas pre-treatment plant (Centro Olio Val d'Agri - COVA) in a populated area, where, so far, ample characterization of aerosol loading is missing. As such, between the 2 and 17 July in 2013, using a number of instruments analyses were carried out on physical, chemical, morphological and optical properties of aerosol at this distinctive site, at both ground and over the atmospheric column, including the investigation of the mixing and transformation of particles. The observation of slag silicates with a rough surface texture is consistent with the presence of oil-related activities which represent the only industrial activity in the area. Desulfurization/sulfur liquefaction processes occurring at COVA can explain the peculiar morphology of calcium-sodium-aluminum particles. The common COVA source was associated with high concentrations of sulfur, nickel and zinc, and with significant correlations between zinc-sulfur and zinc-nickel. The Optical Particle Sizer (OPS) data, hygroscopicity and optical properties of atmospheric aerosol are consistent with the typical oil-derived gaseous emissions (e.g. sulfur dioxide and methane) that strongly influence the mixing state of particles and their size distributions. Continuous combustion processes at COVA were found to be responsible for Equivalent Black Carbon (EBC) concentrations from their relevant contribution to the total number of fine particles. The expected significant contribution of WS (water soluble) and BC (Black Carbon) components to the total Aerosol Optical Depth (AOD) are consistent with the results from the radiometric model especially for July 3 and 16.

  6. Antioxidant efficacy of black pepper (Piper nigrum L.) and piperine in rats with high fat diet induced oxidative stress.

    PubMed

    Vijayakumar, R S; Surya, D; Nalini, N

    2004-01-01

    The present study was aimed to explore the effect of black pepper (Piper nigrum L.) on tissue lipid peroxidation, enzymic and non-enzymic antioxidants in rats fed a high-fat diet. Thirty male Wistar rats (95-115 g) were divided into 5 groups. They were fed standard pellet diet, high-fat diet (20% coconut oil, 2% cholesterol and 0.125% bile salts), high-fat diet plus black pepper (0.25 g or 0.5 g/kg body weight), high-fat diet plus piperine (0.02 g/kg body weight) for a period of 10 weeks. Significantly elevated levels of thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and significantly lowered activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) in the liver, heart, kidney, intestine and aorta were observed in rats fed the high fat diet as compared to the control rats. Simultaneous supplementation with black pepper or piperine lowered TBARS and CD levels and maintained SOD, CAT, GPx, GST, and GSH levels to near those of control rats. The data indicate that supplementation with black pepper or the active principle of black pepper, piperine, can reduce high-fat diet induced oxidative stress to the cells.

  7. Application of microwaves for microbial load reduction in black pepper (Piper nigrum L.).

    PubMed

    Jeevitha, G Chengaiyan; Sowbhagya, H Bogegowda; Hebbar, H Umesh

    2016-09-01

    Black pepper (Piper nigrum L.) is exposed to microbial contamination which could potentially create public health risk and also rejection of consignments in the export market due to non-adherance to microbial safety standards. The present study investigates the use of microwave (MW) radiation for microbial load reduction in black pepper and analyses the effect on quality. Black pepper was exposed to MWs at two different power levels (663 and 800 W) at an intensity of 40 W g(-1) for different time intervals (1-15 min) and moisture content (110 and 260 g kg(-1) on a wet basis). The exposure of black pepper to MWs at 663 W for 12.5 min was found to be sufficient to reduce the microbial load to the permissible level suggested by the International Commission on Microbiological Specifications for Foods and the European Spice Association. The retention of volatile oil, piperine and resin was 91.3 ± 0.03, 87.6 ± 0.02 and 90.7 ± 0.05%, respectively, in MW-treated black pepper. The final moisture content after MW treatment was found to be 100 ± 1 g kg(-1) for black pepper containing initial moisture of 260 ± 3 g kg(-1) . These results suggest that MW heating can be effectively used for microbial load reduction of black pepper without a significant loss in product quality. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  8. Enhancement of nutritionally significant constituents of black currant seeds by chemical elicitor application.

    PubMed

    Flores, Gema; Ruiz del Castillo, María Luisa

    2016-03-01

    Black currant seeds are obtained as a residue during juice production. Black currant seed oil contains high amounts of nutritionally desirable constituents such as γ-linolenic acid (GLA), α-linolenic acid (ALA) and stearidonic acid (SA), as well as certain phenolic acids, which act as natural antioxidants. Fatty acids and phenolic acids of seeds from black currant cultivars after elicitation with methyl jasmonate (MJ) were examined. GLA contents around 25% with respect to total fatty acid content were measured in seeds after pre-harvest treatment of black currants with 0.02mM MJ in 0.05% Tween-20. High GLA samples also exhibited high SA content (higher than 10% with respect to total fatty acid content); however, ALA dropped (from 16% to 10%). High GLA content seeds also showed increased contents of gallic, caffeic, p-coumaric and ferulic acids. In particular, seeds from 0.02mM MJ treated Ben Hope black currants exerted contents of gallic, caffeic, p-coumaric and ferulic acids of 201.4, 125.9, 201.3 and 112.5μgg(-1)vs 124.3, 58.6, 165.4 and 95.8μgg(-1) measured in seeds from untreated Ben Hope black currants. Comparable results were obtained for Ben Alder and Ben Gairn berries. Chemical elicitation with 0.02 MJ is proposed as an industrial practice in such a way that, after consideration of quality issues, it would be obtained high added value black currant seeds. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Western Greece unconventional hydrocarbon potential from oil shale and shale gas reservoirs

    NASA Astrophysics Data System (ADS)

    Karakitsios, Vasileios; Agiadi, Konstantina

    2013-04-01

    It is clear that we are gradually running out of new sedimentary basins to explore for conventional oil and gas and that the reserves of conventional oil, which can be produced cheaply, are limited. This is the reason why several major oil companies invest in what are often called unconventional hydrocarbons: mainly oil shales, heavy oil, tar sand and shale gas. In western Greece exist important oil and gas shale reservoirs which must be added to its hydrocarbon potential1,2. Regarding oil shales, Western Greece presents significant underground immature, or close to the early maturation stage, source rocks with black shale composition. These source rock oils may be produced by applying an in-situ conversion process (ICP). A modern technology, yet unproven at a commercial scale, is the thermally conductive in-situ conversion technology, developed by Shell3. Since most of western Greece source rocks are black shales with high organic content, those, which are immature or close to the maturity limit have sufficient thickness and are located below 1500 meters depth, may be converted artificially by in situ pyrolysis. In western Greece, there are several extensive areas with these characteristics, which may be subject of exploitation in the future2. Shale gas reservoirs in Western Greece are quite possibly present in all areas where shales occur below the ground-water level, with significant extent and organic matter content greater than 1%, and during their geological history, were found under conditions corresponding to the gas window (generally at depths over 5,000 to 6,000m). Western Greece contains argillaceous source rocks, found within the gas window, from which shale gas may be produced and consequently these rocks represent exploitable shale gas reservoirs. Considering the inevitable increase in crude oil prices, it is expected that at some point soon Western Greece shales will most probably be targeted. Exploration for conventional petroleum reservoirs, through the interpretation of seismic profiles and the surface geological data, will simultaneously provide the subsurface geometry of the unconventional reservoirs. Their exploitation should follow that of conventional hydrocarbons, in order to benefit from the anticipated technological advances, eliminating environmental repercussions. As a realistic approach, the environmental consequences of the oil shale and shale gas exploitation to the natural environment of western Greece, which holds other very significant natural resources, should be delved into as early as possible. References 1Karakitsios V. & Rigakis N. 2007. Evolution and Petroleum Potential of Western Greece. J.Petroleum Geology, v. 30, no. 3, p. 197-218. 2Karakitsios V. 2013. Western Greece and Ionian Sea petroleum systems. AAPG Bulletin, in press. 3Bartis J.T., Latourrette T., Dixon L., Peterson D.J., Cecchine G. 2005. Oil Shale Development in the United States: Prospect and Policy Issues. Prepared for the National Energy Tech. Lab. of the U.S. Dept Energy. RAND Corporation, 65 p.

  10. Hematological indices of injury to lightly oiled birds from the Deepwater Horizon oil spill

    USGS Publications Warehouse

    Fallon, Jesse A.; Smith, Eric P.; Schoch, Nina; Paruk, James D.; Adams, Evan A.; Evers, David C.; Jodice, Patrick G. R.; Perkins, Christopher; Schulte, Shiloh A.; Hopkins, William A.

    2018-01-01

    Avian mortality events are common following large‐scale oil spills. However, the sublethal effects of oil on birds exposed to light external oiling are not clearly understood. We found that American oystercatchers (area of potential impact n = 42, reference n = 21), black skimmers (area of potential impact n = 121, reference n = 88), brown pelicans (area of potential impact n = 91, reference n = 48), and great egrets (area of potential impact n = 57, reference n = 47) captured between 20 June 2010 and 23 February 2011 following the Deepwater Horizon oil spill experienced oxidative injury to erythrocytes, had decreased volume of circulating erythrocytes, and showed evidence of a regenerative hematological response in the form of increased reticulocytes compared with reference populations. Erythrocytic inclusions consistent with Heinz bodies were present almost exclusively in birds from sites impacted with oil, a finding pathognomonic for oxidative injury to erythrocytes. Average packed cell volumes were 4 to 19% lower and average reticulocyte counts were 27 to 40% higher in birds with visible external oil than birds from reference sites. These findings provide evidence that small amounts of external oil exposure are associated with hemolytic anemia. Furthermore, we found that some birds captured from the area impacted by the spill but with no visible oiling also had erythrocytic inclusion bodies, increased reticulocytes, and reduced packed cell volumes when compared with birds from reference sites. Thus, birds suffered hematologic injury despite no visible oil at the time of capture. Together, these findings suggest that adverse effects of oil spills on birds may be more widespread than estimates based on avian mortality or severe visible oiling.

  11. Atmospheric cold plasma jet for plant disease treatment

    NASA Astrophysics Data System (ADS)

    Zhang, Xianhui; Liu, Dongping; Zhou, Renwu; Song, Ying; Sun, Yue; Zhang, Qi; Niu, Jinhai; Fan, Hongyu; Yang, Si-ze

    2014-01-01

    This study shows that the atmospheric cold plasma jet is capable of curing the fungus-infected plant leaves and controlling the spread of infection as an attractive tool for plant disease management. The healing effect was significantly dependent on the size of the black spots infected with fungal cells and the leaf age. The leaves with the diameter of black spots of <2 mm can completely recover from the fungus-infected state. The plasma-generated species passing through the microns-sized stomas in a leaf can weaken the function of the oil vacuoles and cell membrane of fungal cells, resulting in plasma-induced inactivation.

  12. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Liyasova, Mariya, E-mail: mliyasov@unmc.edu; Department of Environmental, Agricultural, and Occupational Health, University of Nebraska Medical Center, Omaha, NE; Li, Bin, E-mail: binli@unmc.edu

    The aircraft cabin and flight deck ventilation are supplied from partially compressed unfiltered bleed air directly from the engine. Worn or defective engine seals can result in the release of engine oil into the cabin air supply. Aircrew and passengers have complained of illness following such 'fume events'. Adverse health effects are hypothesized to result from exposure to tricresyl phosphate mixed esters, a chemical added to jet engine oil and hydraulic fluid for its anti-wear properties. Our goal was to develop a laboratory test for exposure to tricresyl phosphate. The assay was based on the fact that the active-site serinemore » of butyrylcholinesterase reacts with the active metabolite of tri-o-cresyl phosphate, cresyl saligenin phosphate, to make a stable phosphorylated adduct with an added mass of 80 Da. No other organophosphorus agent makes this adduct in vivo on butyrylcholinesterase. Blood samples from jet airplane passengers were obtained 24-48 h after completing a flight. Butyrylcholinesterase was partially purified from 25 ml serum or plasma, digested with pepsin, enriched for phosphorylated peptides by binding to titanium oxide, and analyzed by mass spectrometry. Of 12 jet airplane passengers tested, 6 were positive for exposure to tri-o-cresyl phosphate that is, they had detectable amounts of the phosphorylated peptide FGEpSAGAAS. The level of exposure was very low. No more than 0.05 to 3% of plasma butyrylcholinesterase was modified. None of the subjects had toxic symptoms. Four of the positive subjects were retested 3 to 7 months following their last airplane trip and were found to be negative for phosphorylated butyrylcholinesterase. In conclusion, this is the first report of an assay that detects exposure to tri-o-cresyl phosphate in jet airplane travelers. -- Highlights: Black-Right-Pointing-Pointer Travel on jet airplanes is associated with an illness, aerotoxic syndrome. Black-Right-Pointing-Pointer A possible cause is exposure to tricresyl phosphate in engine lubricating oil. Black-Right-Pointing-Pointer A blood test for exposure to tri-o-cresyl phosphate is reported.« less

  13. Prevalence of Salmonella in 11 Spices Offered for Sale from Retail Establishments and in Imported Shipments Offered for Entry to the United States.

    PubMed

    Zhang, Guodong; Hu, Lijun; Pouillot, Régis; Tatavarthy, Aparna; Doren, Jane M Van; Kleinmeier, Daria; Ziobro, George C; Melka, David; Wang, Hua; Brown, Eric W; Strain, Errol; Bunning, Vincent K; Musser, Steven M; Hammack, Thomas S

    2017-10-04

    The U.S. Food and Drug Administration conducted a survey to evaluate Salmonella prevalence and aerobic plate counts in packaged (dried) spices offered for sale at retail establishments in the United States. The study included 7,250 retail samples of 11 spice types that were collected during November 2013 to September 2014 and October 2014 to March 2015. No Salmonella-positive samples (based on analysis of 125 g) were found among retail samples of cumin seed (whole or ground), sesame seed (whole, not roasted or toasted, and not black), and white pepper (ground or cracked), for prevalence estimates of 0.00% with 95% Clopper and Pearson's confidence intervals of 0.00 to 0.67%, 0.00 to 0.70%, and 0.00 to 0.63%, respectively. Salmonella prevalence estimates (confidence intervals) for the other eight spice types were 0.19% (0.0048 to 1.1%) for basil leaf (whole, ground, crushed, or flakes), 0.24% (0.049 to 0.69%) for black pepper (whole, ground, or cracked), 0.56% (0.11 to 1.6%) for coriander seed (ground), 0.19% (0.0049 to 1.1%) for curry powder (ground mixture of spices), 0.49% (0.10 to 1.4%) for dehydrated garlic (powder, granules, or flakes), 0.15% (0.0038 to 0.83%) for oregano leaf (whole, ground, crushed, or flakes), 0.25% (0.03 to 0.88%) for paprika (ground or cracked), and 0.64% (0.17 to 1.6%) for red pepper (hot red pepper, e.g., chili, cayenne; ground, cracked, crushed, or flakes). Salmonella isolates were serotyped, and genomes were sequenced. Samples of these same 11 spice types were also examined from shipments of imported spices offered for entry to the United States from 1 October 2011 to 30 September 2015. Salmonella prevalence estimates (based on analysis of two 375-g composite samples) for shipments of imported spices were 1.7 to 18%. The Salmonella prevalence estimates for spices offered for sale at retail establishments for all of the spice types except dehydrated garlic and basil were significantly lower than estimates for shipments of imported spice offered for entry.

  14. NARMAX model identification of a palm oil biodiesel engine using multi-objective optimization differential evolution

    NASA Astrophysics Data System (ADS)

    Mansor, Zakwan; Zakaria, Mohd Zakimi; Nor, Azuwir Mohd; Saad, Mohd Sazli; Ahmad, Robiah; Jamaluddin, Hishamuddin

    2017-09-01

    This paper presents the black-box modelling of palm oil biodiesel engine (POB) using multi-objective optimization differential evolution (MOODE) algorithm. Two objective functions are considered in the algorithm for optimization; minimizing the number of term of a model structure and minimizing the mean square error between actual and predicted outputs. The mathematical model used in this study to represent the POB system is nonlinear auto-regressive moving average with exogenous input (NARMAX) model. Finally, model validity tests are applied in order to validate the possible models that was obtained from MOODE algorithm and lead to select an optimal model.

  15. Preliminary sediments quality assessment of the Midia Port aquatorium - Black Sea - Romania

    NASA Astrophysics Data System (ADS)

    Catianis, I.; Ungureanu, C.; Stanica, A.

    2012-04-01

    This study shows the present environmental quality of superficial sediments within the aquatorium of an industrial harbor. These results help better understand the correlation between historical pollution and present environmental state of the harbor sediments and related environmental hazards in case of dredging. The study area is the Port of Midia, situated in Romania, western Black Sea coast. The port is located at the boundary between the Danube Delta coast (widest nature protected biosphere reserve in the EU) in the north - and the almost entirely human controlled coast - starting south of the port. Midia Harbor was first built in 1942, expanded during the late 1970`s, and currently operates as an oil and general merchandise terminal. This port receives a double impact of waters: brackish Black Sea waters, as well as Danube fresh waters arriving into the aquatorium through the inshore Danube - Black Sea Canal Lock. To understand the environmental status of the port aquatorium one sampling campaign was performed during August 2011. Samples were collected within the aquatorium and in in the surrounding area outside the harbor. For a better correlation with the particularities related to the transitions in water salinity, the aquatorium was divided into five sectors with specific characteristics: Cargo Terminal Area, Oil Terminal Enclosure Area, Ships Transit Area, Waste Oil Buffer Area and Marine Area. The following analyses were performed on water and sediment samples: physical and chemical characteristics of the waters (dissolved oxygen, temperature, electrical conductivity, total dissolved salts, pH, redox potential, nitrates, nitrites, phosphates, sulphates, heavy metals), bulk sediments (particle size, grain size distribution, mineralogy, organic matter and carbonates content, heavy minerals load, organic compounds) and microbiological and ecotoxicological assays. The grain size analysis reveals the prevalence of very fine-silt and silty-clay fractions, and in a smaller quota sandy fractions. Mineralogically, quartz dominates the sandy and silty fractions of sediments mass, being subsequently followed by a large spectrum of other minerals: feldspar, mica minerals, chlorite, heavy minerals, etc. Besides the mainly siliciclastic mass, sediments are rich in organic matter, with lower contents of carbonates. Some of the sediments are contaminated with petroleum products. All measured water samples were polluted with heavy metals (As, B, Se) and sulphates. Sediments show different contents of chemical compounds, in relation with the spatial distribution of the harbor sectors. Highest concentrations of total volatiles compounds were found in sediments from Waste Oil Buffer Area and Ships Transit Area. Microbiologically contaminated sediments were found in Cargo Terminal Area, Waste Oil Buffer Area and Ships Transit Area. Some samples from Cargo Terminal Area and Ships Transit Area present toxicity signs. Acknowledgments: "This work was supported by the strategic grant POSDRU/89/1.5/S/58852; Project "Postdoctoral program for training scientific researchers" co-financed by the European Social Found within the Sectorial Operational Program Human Resources Development 2007 - 2013", and was performed with scientific and technical assistance provided by NIRD GeoEcoMar - Romania, during the Sedi Port Sil -Project, Life 09ENV/IT/000158

  16. A History of the Army Ground Forces; Study Number 16. The Army Ground Forces History of the Second Army

    DTIC Science & Technology

    1945-09-01

    proficiency in conjunction with other arms and services.7 GHQ and Second Army placed great stress on the development of instructors and the proper training of...separate units. This was clearly brought out in a report of iuspection of Second Army units at Ft. Knox, Ky., by Maj. Gen. J. M. Cumins , Commander of the...covered were food and water, hygiene, housing, field sanitation, and contagious and communicable diseases. Physical exercise stressed mass calisthenics

  17. Historical and Architectural Study of Buildings and Artifacts Associated with the Bulltown Historic Area, Burnsville Lake Project, Braxton County, West Virginia,

    DTIC Science & Technology

    1979-09-01

    consider ways in which troops could be effectively, even humanely employed in light of such developments. The result was an appalling loss of human and...specified by Canonical law and stems from the belief in the sanctity of human remains which is in turn linked to the concept of bodily resurrection. The...Robert Nash 1978, pers. omm.) . Catholic cemeteries are subject to three types of onsecration (Father Robert Nash 1978, pers. cumin .). A Pontifical

  18. Geologic framework of oil and gas genesis in main sedimentary basins from Romania Oprea Dicea

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ionescu, N.; Morariu, C.D.

    1991-03-01

    Oil and gas fields located in Moldavic nappes are encompassed in Oligocene and lower Miocene formations, mostly in the marginal folds nappe, where Kliwa Sandstone sequences have high porosity, and in the Black Sea Plateau. The origin of the hydrocarbon accumulations from the Carpathian foredeep seems to be connected to the Oligocene-lower Miocene bituminous formations of the marginal folds and sub-Carpathian nappes. In the Gethic depression, the hydrocarbon accumulations originate in Oligocene and Miocene source rocks and host in structural, stratigraphical, and lithological traps. The accumulations connected with tectonic lines that outline the areal extension of the Oligocene, Miocene, andmore » Pliocene formations are in the underthrusted Moesian platform. The hydrocarbon accumulations related to the Carpathian foreland represent about 40% of all known accumulations in Romania. Most of them are located in the Moesian platform. In this unit, the oil and gas fields present a vertical distribution at different stratigraphic levels, from paleozoic to Neogene, and in all types of reservoirs, suggesting multicycles of oleogenesis, migration, accumulation, and sealing conditions. The hydrocarbon deposits known so far on the Black Sea continental plateau are confined in the Albian, Cenomanian, Turonian-Senonian, and Eocene formations. The traps are of complex type structural, lithologic, and stratigraphic. The reservoirs are sandstones, calcareous sandstones, limestones, and sands. The hydrocarbon source rocks are pelitic and siltic Oligocene formations. Other older source rocks are probably Cretaceous.« less

  19. Low-angle X-ray scattering properties of irradiated spices

    NASA Astrophysics Data System (ADS)

    Almeida, A. P. G.; Braz, D.; Barroso, R. C.; Lopes, R. T.

    2007-09-01

    The scattering of X-rays at low angles (LAXS) is a technique dominated by the coherent scattering process. One characteristic observation of low-angle coherent scattering is the so-called molecular interference effect, being characterized by the presence of one or more peaks in the forward direction of scattering. In the present study, LAXS profiles from five different spices are carefully measured in order to establish characteristic scattering signatures. Samples of Ceylon cinnamon, cumin, nutmeg, paprika and black pepper were bought in local market in Rio de Janeiro, Brazil. The LAXS patterns were obtained using a Shimadzu DRX 6000 diffractometer in reflection geometry. Coherent scattering patterns are measured for the samples for θ=5-35°. The data were collected in 0.05° increments every 3 s. In order to evaluate the possible molecular structure changes caused to the irradiation procedure, the signatures obtained for control (non-irradiated) spices were compared with spice samples irradiated with different doses varying from 3 to 40 kGy. The LAXS patterns of all samples were obtained after 30, 60, 90, 120 days to evaluate the effect of storage period. Scattering profiles from spices irradiated with different irradiation doses were obtained and the results compared. For each spice, there is no considerable deviation in shape in function of the irradiation dose. It indicates that the molecular structure of each analyzed spices is preserved considering the dose range chosen. The results show that the molecular structure was found to be stable during storage at the ambient temperature for up to 4 months.

  20. Dietary sources of aldose reductase inhibitors: prospects for alleviating diabetic complications.

    PubMed

    Saraswat, Megha; Muthenna, P; Suryanarayana, P; Petrash, J Mark; Reddy, G Bhanuprakash

    2008-01-01

    Activation of polyol pathway due to increased aldose reductase activity is one of the several mechanisms that have been implicated in the development of various secondary complications of diabetes. Though numerous synthetic aldose reductase inhibitors have been tested, these have not been very successful clinically. Therefore, a number of common plant/ natural products used in Indian culinary have been evaluated for their aldose reductase inhibitory potential in the present study. The aqueous extracts of 22 plant-derived materials were prepared and evaluated for the inhibitory property against rat lens and human recombinant aldose reductase. Specificity of these extracts towards aldose reductase was established by testing their ability to inhibit a closely related enzyme viz, aldehyde reductase. The ex vivo incubation of erythrocytes in high glucose containing medium was used to underscore the significance in terms of prevention of intracellular sorbitol accumulation. Among the 22 dietary sources tested, 10 showed considerable inhibitory potential against both rat lens and human recombinant aldose reductase. Prominent inhibitory property was found in spinach, cumin, fennel, lemon, basil and black pepper with an approximate IC50 of 0.2 mg/mL with an excellent selectivity towards aldose reductase. As against this, 10 to 20 times higher concentrations were required for 50% inhibition of aldehyde reductase. Reduction in the accumulation of intracellular sorbitol by the dietary extracts further substantiated their in vivo efficacy. The findings reported here indicate the scope of adapting life-style modifications in the form of inclusion of certain common sources in the diet for the management of diabetic complications.

  1. Effect of administering black cumin (Nigella sativa) toward postpartum mice (MusMusculus L.)

    NASA Astrophysics Data System (ADS)

    Imelda, F.; Darti, N. A.

    2018-03-01

    The period of childbirth is a period for the health provider monitoring that less monitoring can cause the mother to suffer a variety of problemsandcomplications during childbirth such as post-partum infections. This type of research was an experimental group P0: control group, treatment groups by administering Nigella sativa P1:2.6mg/day, P2:3.9mg/day, P3:5.2mg/day, and P4:6.5mg/day, which each group 5 samples. The average amount of leukocytes after given Nigella sativa 2.6mg/day for seven days (P1) which was 7:10±0:57 (x103cells/mm3), and at least in female mice after given Nigella sativa 6.5mg/day for sevendays (P4) which was 6.62±0.52 (x103cells/mm3). The average amount lymphocytes after given Nigella sativa 2.6mg/day for seven days (P1) which was 63.40±4.77 (x103cells/mm3), and least in female mice after given Nigella sativa 3.9 mg/day for seven days (P3) which was 47.00±14:58 (x103cells/mm3). Amount of monocytes after given Nigella sativa 5.2mg/day for seven days (P3) which was 5.40±0.55 (x103cells/mm3), and least in female mice after given Nigella sativa 2.6mg/day for seven days (P1) which was 4.80±1.30 (x103cells/mm3).

  2. Wind impact on the Black Sea ecosystem

    NASA Astrophysics Data System (ADS)

    Stanichny, Sergey; Ratner, Yuriy; Shokurov, Mike; Stanychna, Rimma; Soloviev, Dmytro; Burdyugov, Vyacheslav

    2010-05-01

    Combination of the recent satellite and meteorological data for the regional investigation allowed to describe new features of the processes in marine ecosystem and detect some relations with wind variability for different time scales. Next topics are highlighted in presentation: 1. Inter-annual variability of the wind stress curl over the Black Sea. Shift in the atmospheric processes after 2003 year and related variations in chlorophyll concentration and intensity of the mesoscale currents. 2. Like-tropical cyclone in September 2005 and its impact o the Black Sea upper layer. 3. Strong storm November 11, 2007 and oil pollutions of the Kerch Strait. 4. Relation of the Danube waters transport with wind fields for summer 2007 and 2008. 5. "Valley" wind in the Eastern part of the Black Sea and its impact on the Rim current formation. 6. Low wind conditions and blue -green algae bloom. NCEP, SKIRON and MHI MM5 wind data together with AVHRR, MODIS, MERIS, ETM+, QuikSCAT, ASAR (ESA) satellite data were used for investigation. Work was done with support of the SESAME FP7, "Stable Ecosystem" and Operational Oceanography NASU projects.

  3. Oil and gas impacts on air quality in federal lands in the Bakken region: an overview of the Bakken Air Quality Study and first results

    NASA Astrophysics Data System (ADS)

    Prenni, A. J.; Day, D. E.; Evanoski-Cole, A. R.; Sive, B. C.; Hecobian, A.; Zhou, Y.; Gebhart, K. A.; Hand, J. L.; Sullivan, A. P.; Li, Y.; Schurman, M. I.; Desyaterik, Y.; Malm, W. C.; Schichtel, B. A.; Collett, J. L., Jr.

    2015-10-01

    The Bakken formation contains billions of barrels of oil and gas trapped in rock and shale. Horizontal drilling and hydraulic fracturing methods have allowed for extraction of these resources, leading to exponential growth of oil production in the region over the past decade. Along with this development has come an increase in associated emissions to the atmosphere. Concern about potential impacts of these emissions on federal lands in the region prompted the National Park Service to sponsor the Bakken Air Quality Study over two winters in 2013-2014. Here we provide an overview of the study and present some initial results aimed at better understanding the impact of local oil and gas emissions on regional air quality. Data from the study, along with long term monitoring data, suggest that while power plants are still an important emissions source in the region, emissions from oil and gas activities are impacting ambient concentrations of nitrogen oxides and black carbon and may dominate recent observed trends in pollutant concentrations at some of the study sites. Measurements of volatile organic compounds also definitively show that oil and gas emissions were present in almost every air mass sampled over a period of more than four months.

  4. Oil and gas impacts on air quality in federal lands in the Bakken region: an overview of the Bakken Air Quality Study and first results

    NASA Astrophysics Data System (ADS)

    Prenni, A. J.; Day, D. E.; Evanoski-Cole, A. R.; Sive, B. C.; Hecobian, A.; Zhou, Y.; Gebhart, K. A.; Hand, J. L.; Sullivan, A. P.; Li, Y.; Schurman, M. I.; Desyaterik, Y.; Malm, W. C.; Collett, J. L., Jr.; Schichtel, B. A.

    2016-02-01

    The Bakken formation contains billions of barrels of oil and gas trapped in rock and shale. Horizontal drilling and hydraulic fracturing methods have allowed for extraction of these resources, leading to exponential growth of oil production in the region over the past decade. Along with this development has come an increase in associated emissions to the atmosphere. Concern about potential impacts of these emissions on federal lands in the region prompted the National Park Service to sponsor the Bakken Air Quality Study over two winters in 2013-2014. Here we provide an overview of the study and present some initial results aimed at better understanding the impact of local oil and gas emissions on regional air quality. Data from the study, along with long-term monitoring data, suggest that while power plants are still an important emissions source in the region, emissions from oil and gas activities are impacting ambient concentrations of nitrogen oxides and black carbon and may dominate recent observed trends in pollutant concentrations at some of the study sites. Measurements of volatile organic compounds also definitively show that oil and gas emissions were present in almost every air mass sampled over a period of more than 4 months.

  5. Colored shade nets induced changes in growth, anatomy and essential oil of Pogostemon cablin.

    PubMed

    Ribeiro, Aurislaine S; Ribeiro, Mariana S; Bertolucci, Suzan K V; Bittencourt, Wanderley J M; Carvalho, Alexandre A DE; Tostes, Wesley N; Alves, Eduardo; Pinto, José E B P

    2018-04-16

    The purpose of this investigation was to determine the influence of colored shade nets on the growth, anatomy and essential oil content, yield and chemical composition of Pogostemon cablin. The plants were cultivated under full sunlight, black, blue and red nets. The harvesting was performed 5 months after planting and it was followed by the analysis of plant growth parameters, leaf anatomy, essential oil content, yield and chemical composition. The plants grown under red net have produced more leaf, shoot, total dry weight and leaf area. Plants cultivated under colored nets showed differences in morphological features. Plants maintained under red net had a higher leaf blade thickness and polar and equatorial diameter of the stomata ratio. Additionally, higher yield of essential oil in the leaves was observed under red and blue colored shade net. The essential oil of the plants grown under red net showed the highest relative percentage of patchoulol (66.84%). Therefore, it is possible using colored shade nets to manipulate P. cablin growth, as well as its essential oil production with several chemical compositions. The analyses of principal components allowed observing that pogostol has negative correlation with α-guaiene and α-bulnesene. There was difference in total dry weight and patchoulol content when the patchouli is cultured under the red colored shade nets.

  6. Chemical Composition and Insecticidal Activity of Essential Oils from Zanthoxylum dissitum Leaves and Roots against Three Species of Storage Pests.

    PubMed

    Wang, Cheng-Fang; Yang, Kai; You, Chun-Xue; Zhang, Wen-Juan; Guo, Shan-Shan; Geng, Zhu-Feng; Du, Shu-Shan; Wang, Yong-Yan

    2015-05-04

    This work aimed to investigate chemical composition of essential oils obtained from Zanthoxylum dissitum leaves and roots and their insecticidal activities against several stored product pests, namely the cigarette beetle (Lasioderma serricorne), red flour beetle (Tribolium castaneum) and black carpet beetle (Attagenus piceus). The analysis by GC-MS of the essential oils allowed the identification of 28 and 22 components, respectively. It was found that sesquiterpenoids comprised a fairly high portion of the two essential oils, with percentages of 74.0% and 80.9% in the leaves and roots, respectively. The main constituents identified in the essential oil of Z. dissitum leaves were δ-cadinol (12.8%), caryophyllene (12.7%), β-cubebene (7.9%), 4-terpineol (7.5%) and germacrene D-4-ol (5.7%), while humulene epoxide II (29.4%), caryophyllene oxide (24.0%), diepicedrene-1-oxide (10.7%) and Z,Z,Z-1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (8.7%) were the major components in the essential oil of Z. dissitum roots. The insecticidal activity results indicated that the essential oil of Z. dissitum roots exhibited moderate contact toxicity against three species of storage pests, L. serricorne,T. castaneum and A. piceus, with LD50 values of 13.8, 43.7 and 96.8 µg/adult, respectively.

  7. Black pepper and health claims: a comprehensive treatise.

    PubMed

    Butt, Masood Sadiq; Pasha, Imran; Sultan, Muhammad Tauseef; Randhawa, Muhammad Atif; Saeed, Farhan; Ahmed, Waqas

    2013-01-01

    For millennia, spices have been an integral part of human diets and commerce. Recently, the widespread recognition of diet-health linkages bolsters their dietary importance. The bioactive components present in them are of considerable significance owing to their therapeutic potential against various ailments. They provide physiological benefits or prevent chronic ailment in addition to the fundamental nutrition and often included in the category of functional foods. Black pepper (Piper Nigrum L.) is an important healthy food owing to its antioxidant, antimicrobial potential and gastro-protective modules. Black pepper, with piperine as an active ingredient, holds rich phytochemistry that also includes volatile oil, oleoresins, and alkaloids. More recently, cell-culture studies and animal modeling predicted the role of black pepper against number of maladies. The free-radical scavenging activity of black pepper and its active ingredients might be helpful in chemoprevention and controlling progression of tumor growth. Additionally, the key alkaloid components of Piper Nigrum, that is, piperine assist in cognitive brain functioning, boost nutrient's absorption and improve gastrointestinal functionality. In this comprehensive treatise, efforts are made to elucidate the antioxidant, antimicrobial, anti-inflammatory, gastro-protective, and antidepressant activities of black pepper. Moreover, the synergistic interaction of black pepper with different drugs and nutrients is the limelight of the manuscript. However, the aforementioned health-promoting benefits associated with black pepper are proven in animal modeling. Thus, there is a need to conduct controlled randomized trials in human subjects, cohort studies, and meta-analyses. Such future studies would be helpful in recommending its application in diet-based regimens to prevent various ailments.

  8. Degradation of black liquor from bioethanol process using coagulation and Fenton-like methods

    NASA Astrophysics Data System (ADS)

    Muryanto, Muryanto; Sari, Ajeng Arum; Abimanyu, Haznan

    2017-01-01

    Black liquor is one of the main by-products of the pretreatment process in bioethanol production from oil palm empty fruit bunches. Black liquor wastewater releases black coloured effluent with high chemical oxygen demand (COD) and low dissolved oxygen (DO). It had a distinctive dark coloration, high alkalinity (pH=13), high organic content (COD > 50,000 ppm) and a high solid content (TSS > 5,000 ppm). Lignin destruction can be done by using high oxidation from OH radical system such as advanced oxidation processes (AOPs). Thereafter, the high concentration of COD, color, and TSS can be removed. The general aim of the present investigation was to determine degradation of black liquor wastewater by using a combined coagulation and Fenton-like methods. In this research, we use Poly Aluminum Chloride (PAC) as a coagulant and FeCl3.6H2O and H2O2 for Fenton-like's reagent. The process was conducted in jar test at 200 rpm for 30 minutes and after that slowly mixed for 2 hours and left for sedimentation 24 hours. 50 ml black liquor was added with variation dose of 1-5% PAC, and 10 % Fenton-like reagent. Hydroxyl radical was generated by the Fenton-like's reagent (ratio FeCl3.6H2O : H2O2 was varied). The highest decolorization of black liquor 70 % was obtained under 5% PAC coagulant. The pH of the wastewater was reduced from 13.00 to 8.07 after the addition of the coagulant. The decolorization of original black liquor was approximately 58% through the Fenton-like process. The combination of PAC and Fenton-like reagent has able to enhance the decolorization of black liquor up to 97%.

  9. Repellence of plant essential oils to Dermanyssus gallinae and toxicity to the non-target invertebrate Tenebrio molitor.

    PubMed

    George, D R; Sparagano, O A E; Port, G; Okello, E; Shiel, R S; Guy, J H

    2009-05-26

    With changes in legislation and consumer demand, alternatives to synthetic acaricides to manage the poultry red mite Dermanyssus gallinae (De Geer) in laying hen flocks are increasingly needed. These mites may cause losses in egg production, anaemia and even death of hens. It may be possible to use plant-derived products as D. gallinae repellents, especially if such products have a minimal impact on non-target organisms. An experiment was conducted with D. gallinae to assess the repellence of a range of plant essential oils, previously found to be of varying toxicity (relatively highly toxic to non-toxic) to this pest. Experiments were also undertaken to assess the toxicity of these products to mealworm beetles (Tenebrio molitor L.), a non-target invertebrate typical of poultry production systems. Results showed that all seven essential oils tested (manuka, thyme, palmarosa, caraway, spearmint, black pepper and juniper leaf) were repellent to D. gallinae at 0.14mg oil/cm(3) (initial concentration) during the first 2 days of study. Thyme essential oil appeared to be the most effective, where repellence lasted until the end of the study period (13 days). At the same concentration toxicity to T. molitor differed, with essential oils of palmarosa and manuka being no more toxic to adult beetles than the control. There was neither a significant association between the rank toxicity and repellence of oils to D. gallinae, nor the toxicity of oils to D. gallinae (as previously determined) and T. molitor.

  10. The use of hydroxyacids as geochemical indicators

    NASA Technical Reports Server (NTRS)

    Cardoso, J. N.; Eglinton, G.

    1983-01-01

    The distributions of hydroxyacids in a variety of recent and ancient sediments have been determined by gas chromatography-mass spectrometry. A lacustrine sediment (Rostherne Mere, U.K.) and two marine situations (Cariaco Trench, Black Sea) were analyzed as examples of recent depositional environments. Beta-hydroxyacids occurred in all of the recent sediments, consistent with their presumed microbial origin, whereas di- and trihydroxyacids were present in the sediments with a significant higher plant input (Black Sea and Rostherne Mere sediments). The two ancient (Eocene) sediments examined, viz, the Greene River (U.S.) and Messel (Germany) oil shales, contained only trace amounts of hydroxyacids. These results provide evidence that hydroxyacids are in general not well preserved over geological time.

  11. Organic geochemistry and petrology of oil source rocks, Carpathian Overthrust region, southeastern Poland - Implications for petroleum generation

    USGS Publications Warehouse

    Kruge, M.A.; Mastalerz, Maria; Solecki, A.; Stankiewicz, B.A.

    1996-01-01

    The organic mailer rich Oligocene Menilite black shales and mudstones are widely distributed in the Carpathian Overthrust region of southeastern Poland and have excellent hydrocarbon generation potential, according to TOC, Rock-Eval, and petrographic data. Extractable organic matter was characterized by an equable distribution of steranes by carbon number, by varying amounts of 28,30-dinor-hopane, 18??(H)-oleanane and by a distinctive group of C24 ring-A degraded triterpanes. The Menilite samples ranged in maturity from pre-generative to mid-oil window levels, with the most mature in the southeastern portion of the study area. Carpathian petroleum samples from Campanian Oligocene sandstone reservoirs were similar in biomarker composition to the Menilite rock extracts. Similarities in aliphatic and aromatic hydrocarbon distributions between petroleum asphaltene and source rock pyrolyzates provided further evidence genetically linking Menilite kerogens with Carpathian oils.

  12. Sizing Determination Final Report

    DTIC Science & Technology

    1988-02-01

    A Name: ERIC WHEATLEY Subject No.: 4 S/N: ------------ Sex.: M Race: BLACK Age...z:ni:zum Frcx-tal Arc - Tahc and -Marker Tool 19.= 6- - B-prozy.ncmatic_ ,’entlon Ar- - T"p_ and Marler Tool ......... .. 26.:; i7 Bitragion Mini-o-u- Frc...tape and Marler Tool ............. 24.5 7 . ilt’-aoion inigmu F-ontal Ar-c - -:•c= Oil- ---................... . ?.. S:-.-_,_ . ................. 1

  13. 40 CFR 180.641 - Spirotetramat; tolerances for residues.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Acerola 2.5 Almond, hulls 9.0 Aspirated grain fractions 10.0 Avocado 0.60 Black sapote 0.60 Brassica, head and stem, subgroup 5A 2.5 Brassica, leafy, subgroup 5B 8.0 Canistel 0.60 Citrus, oil 6.0 Cotton gin... 7.0 Vegetable, fruiting, group 8 2.5 Vegetable, leafy, except brassica, group 4 9.0 Vegetable...

  14. Essential Oils as Biocides for the Control of Fungal Infections and Devastating Pest (Tuta absoluta) of Tomato (Lycopersicon esculentum Mill.).

    PubMed

    Bouayad Alam, Samira; Dib, Mohammed El Amine; Djabou, Nassim; Tabti, Boufeldja; Gaouar Benyelles, Nassira; Costa, Jean; Muselli, Alain

    2017-07-01

    Thymus capitatus and Tetraclinis articulata essential oils as well their major components (carvacrol and α-pinene) were evaluated for their antifungal and insecticidal activities. Both oils showed good in vitro antifungal activity against Fusarium oxysporum, Aspergillus niger, Penicillium sp., Alternaria alternata, and Botrytis cinerea, the fungi causing tomato rot. In vivo results indicate the efficacies of both essential oils and carvacrol of reduce postharvest fungal pathogens, such as B. cinerea and Al. alternata that are responsible of black and gray rot of tomato fruit. Disease incidence of Al. alternata and B. cinerea decreased on average from 55% to 80% with essential oil of Th. capitatus and pure carcvacrol, while Te. articulata essential oil exhibited inhibition of fungal growth of 55% and 25% against Al. alternata and B. cinerea, respectively, with concentration of 0.4 μl/l air. The insecticidal activity of Th. capitatus and Te. articulata essential oils exhibited also a good insecticidal activity. At the concentration of 0.2 μl/ml air, the oils caused mortality over 80% for all larval stages of Tuta absoluta and 100% mortality for the first-instar after 1.5 h only of exposure. α-Pinene presented lower insecticidal and antifungal activities compared to essential oils of Th. capitatus, Te. articulata and pure carvacrol. Thus, these essential oils can be used as a potential source to develop control agents to manage some of the main pests and fungal diseases of tomato crops. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  15. The discovery and development of the El Dorado (Kansas) oil field

    USGS Publications Warehouse

    Skelton, L.H.

    1997-01-01

    Pioneers named El Dorado, Kansas, in 1857 for the beauty of the site and the promise of future riches but not until 58 years later was black rather than mythical yellow gold discovered when the Stapleton No. 1 oil well came in on October 5, 1915. El Dorado's leaders were envious when nearby towns found huge gas fields and thrived. John Donley, an El Dorado barber, had tried to find either gas or oil in 1878 at a nearby site selected by a spiritualist. He staked out a townsite, spudded a well and drilled 200 feet before running out of money. Wells in 1879 and 1882 produced only brine. In June, 1914, chafed over discovery of oil in nearby Augusta, El Dorado city fathers contracted with Erasmus Haworth, soon to retire from his position as State Geologist, to perform a geological study of the area. His field work outlined the El Dorado Anticline, which unsuccessfully was drilled first in August, 1915. On abandonment, the Wichita Natural Gas Company purchased the lease and drilled the Stapleton No. 1 oil well. More success followed and by 1918, the El Dorado produced 29 million barrels, almost 9% of the nation's oil. Entrepreneurs came and prospered: the Cities Service Oil Company, A.L. Derby, Jack Vickers, and Bill Skelly all became familiar names in Midcontinent oil marketing. Earlier giant fields had hurt the price of crude oil but the El Dorado came in as both World War I and the rapid popularization of motor transport made a market for both light and heavy ends of the refinery stream. The giant gas field never materialized as hoped but in late 1995, the El Dorado Field produced its 300 millionth barrel of oil.

  16. Crew Earth Observations (CEO) taken during Expedition 8

    NASA Image and Video Library

    2004-03-24

    ISS008-E-18938 (24 March 2004) --- This photo of a large black smoke plume over southeastern Iraq was taken by an Expedition 8 crewmember on the International Space Station. The oil that is burning spilled from a ruptured pipeline on March 23, 2004. This photo was taken the next afternoon and shows details of land use near the fire. The delta of the Shatt al Arab has numerous channels, estuaries, and tidal flats. Near the top of the photo (southwest) are two large white salt evaporator operations while to the lower right are wetlands and irrigated agricultural fields. Iraq’s only oil terminal on the Persian Gulf is near the left edge of the plume.

  17. Simulation study of the carbon dioxide enhanced oil recovery pilot test in the Griffithsville Field, Lincoln County, West Virginia

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brummert, A.C.

    1990-09-01

    A carbon dioxide pilot test was conducted in the Griffithsville Field, Lincoln County, West Virginia, on a 90-acre tract containing nine 10-acre, normal, five-spot patterns arranged in a 3 {times} 3 matrix. This post-flood simulation study evaluates the initial pressure buildup phase of water injection, the carbon dioxide injection phase, and the chase water injection phase. Core data, geophysical well logs, fluid property data, well test data, and injection/production histories were used in setting up the data input record for the reservoir simulator. The reservoir simulator was IMEX, a four-component, black-oil reservoir simulator. 23 refs., 15 figs., 3 tabs.

  18. Integrated options for the management of black root rot of strawberry caused by Rhizoctonia solani Kuhn.

    PubMed

    Asad-Uz-Zaman, Md; Bhuiyan, Mohammad Rejwan; Khan, Mohammad Ashik Iqbal; Alam Bhuiyan, Md Khurshed; Latif, Mohammad Abdul

    2015-02-01

    An investigation was made to manage strawberry black root rot caused by Rhizoctonia solani (R. solani) through the integration of Trichoderma harzianum (T. harzianum) isolate STA7, mustard oil cake and Provax 200. A series of preliminary experiments were conducted to select a virulent isolate of R. solani, an effective isolate of T. harzianum, a suitable organic amendment, and a suitable fungicide before setting the experiment for integration. The pathogenicity of the selected four isolates of R. solani was evaluated against strawberry and isolate SR1 was selected as the test pathogen due to its highest virulent (95.47% mortality) characteristics. Among the 20 isolates of T. harzianum, isolate STA7 showed maximum inhibition (71.97%) against the test pathogen (R. solani). Among the fungicides, Provax-200 was found to be more effective at lowest concentration (100 ppm) and highly compatible with Trichoderma isolates STA7. In the case of organic amendments, maximum inhibition (59.66%) of R. solani was obtained through mustard oil cake at the highest concentration (3%), which was significantly superior to other amendments. Minimum percentages of diseased roots were obtained with pathogen (R. solani)+Trichoderma+mustard oil cake+Provax-200 treatment, while the highest was observed with healthy seedlings with a pathogen-inoculated soil. In the case of leaf and fruit rot diseases, significantly lowest infected leaves as well as fruit rot were observed with a pathogen+Trichoderma+mustard oil cake+Provax-200 treatment in comparison with the control. A similar trend of high effectiveness was observed by the integration of Trichoderma, fungicide and organic amendments in controlling root rot and fruit diseases of strawberry. Single application of Trichoderma isolate STA7, Provax 200 or mustard oil cake did not show satisfactory performance in terms of disease-free plants, but when they were applied in combination, the number of healthy plants increased significantly. The result of the current study suggests the superiority of our integrated approach to control the sclerotia forming pathogen R. solani compared to the individual treatment either by an antagonist or by a fungicide or by mustard oil cake. Copyright © 2014 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  19. Quality improvement by batch vacuum distillation and physicochemical characterization of clove leaf oil in Central Java, Indonesia

    NASA Astrophysics Data System (ADS)

    Alighiri, D.; Eden, W. T.; Cahyono, E.; Supardi, K. I.

    2018-03-01

    Clove leaf oil is one of the essential oils which have high economic value and produced in considerable amount in Indonesia. As many as 60% of clove oil produced by Indonesia has exported to many countries in the world. Musuk and Ringinlarik Village, Boyolali District, Central Java Province is one of the areas in Indonesia which became the primary supplier of Indonesian clove leaf oil. Although, the quality of the resulting yield is still low because it uses a simple distillation kettle in the form of the iron plate or used the drum. The clove leaf oil produced by steam distillation from the dry whole leaves of the clove tree. The color of the oil is brownish black and dirty with the odor are fresh distilled, spicy, warm, and terpenic. The specific gravity at 25 °C of 1.529, the refractive index at 20 °C of 1.030, and based on GC-MS analysis, eugenol levels only 68% and caryophyllene is too high, i.e., 20%. The quality of clove leaf oil produced does not meet the specifications of international market standards. This work aimed to improve the quality of Indonesian clove leaf oil. The purifications done in this research was used by batch vacuum distillation with mode operation at vacuum -76 cmHg and reflux ratios 5:1. Clove leaf oil produced by using this method has a better physicochemical characterization, i.e., the appearance that is yellow to pale color with the odor is spicy, woody, warm, and terpenic. The specific gravity at 25 °C of 1.533, the refractive index at 20 °C of 1.038, and eugenol and caryophyllene contents has yielded 80.58% and 10%, respectively. By The enhancement quality of clove leaf oil by batch vacuum distillation, these oil is already meet international standards and income of clove leaf oil grower in Musuk and Ringinlarik Village, Boyolali District, Central Java Province, Indonesia could be increased.

  20. Impact of heavy metals on the oil products biodegradation process.

    PubMed

    Zukauskaite, Audrone; Jakubauskaite, Viktorija; Belous, Olga; Ambrazaitiene, Dalia; Stasiskiene, Zaneta

    2008-12-01

    Oil products continue to be used as a principal source of energy. Wide-scale production, transport, global use and disposal of petroleum have made them major contaminants in prevalence and quantity in the environment. In accidental spills, actions are taken to remove or remediate or recover the contaminants immediately, especially if they occur in environmentally sensitive areas, for example, in coastal zones. Traditional methods to cope with oil spills are confined to physical containment. Biological methods can have an advantage over the physical-chemical treatment regimes in removing spills in situ as they offer biodegradation of oil fractions by the micro-organisms. Recently, biological methods have been known to play a significant role in bioremediation of oil-polluted coastal areas. Such systems are likely to be of significance in the effective management of sensitive coastal ecosystems chronically subjected to oil spillage. For this reason the aim of this paper is to present an impact of Mn, Cu, Co and Mo quantities on oil biodegradation effectiveness in coastal soil and to determine the relationship between metal concentrations and degradation of two oil products (black oil and diesel fuel). Soil was collected in the Baltic Sea coastal zone oil products degradation area (Klaipeda, Lithuania). The experiment consisted of two parts: study on the influence of micro-elements on the oil product biodegradation process; and analysis of the influence of metal concentration on the number of HDMs. The analysis performed and results obtained address the following areas: impact of metal on a population of hydrocarbon degrading micro-organisms, impact of metals on residual concentrations of oil products, influence of metals on the growth of micro-organisms, inter-relation of metal concentrations with degradation rates. Statistical analysis was made using ;Statgraphics plus' software. The influence of metals on the growth of micro-organisms, the biodegradation process rate and the oil product concentrations were evaluated with analysis of variance. The impact has been investigated separately and synergetically.

  1. Characterization and Discrimination of Oueslati Virgin Olive Oils from Adult and Young Trees in Different Ripening Stages Using Sterols, Pigments, and Alcohols in Tandem with Chemometrics.

    PubMed

    Chtourou, Fatma; Jabeur, Hazem; Lazzez, Ayda; Bouaziz, Mohamed

    2017-05-03

    Dynamics of squalene, sterol, aliphatic alcohol, pigment, and triterpenic diol accumulations in olive oils from adult and young trees of the Oueslati cultivar were studied for two consecutive years, 2013-2014 and 2014-2015. Data were compared statistically for differences by age of trees, maturation of olive, and year of harvesting. Results showed that the mean campesterol content in olive oil from adult trees at the green stage of maturation was significantly (p < 0.02) above the limit established by IOC legislation. However, the mean values of campesterol and Δ-7-stigmastenol were significantly (p < 0.01) above the limits in oils from young trees at the black stage of ripening. Principal component analysis was applied to alcohols, squalene, pigments, and sterols having noncompliance with the legislation. Then, data of 36 samples were subjected to a discriminant analysis with "maturation" as grouping variable and principal components as input variables. The model revealed clear discrimination of each tree age/maturation stage group.

  2. Simultaneous Determination of Perfluorinated Compounds in Edible Oil by Gel-Permeation Chromatography Combined with Dispersive Solid-Phase Extraction and Liquid Chromatography-Tandem Mass Spectrometry.

    PubMed

    Yang, Lili; Jin, Fen; Zhang, Peng; Zhang, Yanxin; Wang, Jian; Shao, Hua; Jin, Maojun; Wang, Shanshan; Zheng, Lufei; Wang, Jing

    2015-09-30

    A simple analytical method was developed for the simultaneous analysis of 18 perfluorinated compounds (PFCs) in edible oil. The target compounds were extracted by acetonitrile, purified by gel permeation chromatography (GPC) and dispersive solid-phase extraction (DSPE) using graphitized carbon black (GCB) and octadecyl (C18), and analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ES-MS/MS) in negative ion mode. Recovery studies were performed at three fortification levels. The average recoveries of all target PFCs ranged from 60 to 129%, with an acceptable relative standard deviation (RSD) (1-20%, n = 3). The method detection limits (MDLs) ranged from 0.004 to 0.4 μg/kg, which was significantly improved compared with the existing liquid-liquid extraction and cleanup method. The method was successfully applied for the analysis of all target PFCs in edible oil samples collected from markets in Beijing, China, and the results revealed that C6-C10 perfluorocarboxylic acid (PFCAs) and C7 perfluorosulfonic acid PFSAs were the major PFCs detected in oil samples.

  3. Composition, antimicrobial and antioxidant activities of seven essential oils from the North American boreal forest.

    PubMed

    Poaty, Bouddah; Lahlah, Jasmina; Porqueres, Félicia; Bouafif, Hassine

    2015-06-01

    Essential oils (EOs) were steam-extracted from the needles and twigs of balsam fir, black spruce, white spruce, tamarack, jack pine and eastern white cedar that remained after logging in eastern Canada. These EOs, similarly to that from Labrador tea and other commercial EOs from Chinese cinnamon, clove and lemon eucalyptus, exhibited many common constituent compounds (mainly α-pinene, β-pinene, limonene and bornyl acetate) making up 91% of each oil based on gas chromatography-mass spectrometry analysis. All of these oils exhibited antibacterial properties, especially when examined in closed tube assay compared to the traditional 96-well microliter format. These antimicrobial activities (minimum inhibitory concentration ≥ 0.2% w/v), comparable to those of exotic EOs, were shown against common pathogenic bacteria and fungi. The antioxidant potential of the boreal samples was determined by the 1,1-diphenyl-2-picrylhydrazyl radical scavenging (concentration providing 50% inhibition ≥ 7 mg/ml) and reducing power methods. Finally, this investigation revealed some boreal EOs to be potential antimicrobial and antioxidant agents that would notably benefit products in the personal hygiene and care industry.

  4. Late diagenetic indicators of buried oil and gas: II, Direct detection experiment at Cement and Garza oil fields, Oklahoma and Texas, using enhanced LANDSAT I and II images

    USGS Publications Warehouse

    Donovan, Terrence J.; Termain, Patricia A.; Henry, Mitchell E.

    1979-01-01

    The Cement oil field, Oklahoma, was a test site for an experiment designed to evaluate LANDSAT's capability to detect an alteration zone in surface rocks caused by hydrocarbon microseepage. Loss of iron and impregnation of sandstone by carbonate cements and replacement of gypsum by calcite are the major alteration phenomena at Cement. The bedrock alterations are partially masked by unaltered overlying beds, thick soils, and dense natural and cultivated vegetation. Interpreters biased by detailed ground truth were able to map the alteration zone subjectively using a magnified, filtered, and sinusoidally stretched LANDSAT composite image; other interpreters, unbiased by ground truth data, could not duplicate that interpretation. Similar techniques were applied at a secondary test site (Garza oil field, Texas), where similar alterations in surface rocks occur. Enhanced LANDSAT images resolved the alteration zone to a biased interpreter and some individual altered outcrops could be mapped using higher resolution SKYLAB color and conventional black and white aerial photographs suggesting repeat experiments with LANDSAT C and D.

  5. STS-45 Earth observation of the Persian Gulf and the island of Abu Ali

    NASA Technical Reports Server (NTRS)

    1992-01-01

    STS-45 Earth observation taken aboard Atlantis, Orbiter Vehicle (OV) 104, is of the northern reaches of the Persian Gulf with the sunglint pattern centered on the Saudi Arabian island of Abu Ali. Bright features along the coast are thought to be deposits of oil, released from a terminal offshore of Kuwait during the recent Persian Gulf War. Further up the coast, in Kuwait, the black, oil-soaked desert surrounding the site of the oil well fires is clearly visible. View was taken from an altitude of 160 nautical miles with OV-104 located at 28 degrees north and 52.8 degrees east. During the STS-45 mission, an international survey team focused on oil contamination of the shallow-water habitants in the area north of Abu Ali Island. Crewmembers contacted the NOAA survey vessel, the R/V Mt. Mitchell, several times and photographed water color and sunglint within the study area and throughout the entire Persian Gulf. These photographic data are expected to aid the Persian Gulf researchers in

  6. Integrated Mid-Continent Carbon Capture, Sequestration & Enhanced Oil Recovery Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brian McPherson

    2010-08-31

    A consortium of research partners led by the Southwest Regional Partnership on Carbon Sequestration and industry partners, including CAP CO2 LLC, Blue Source LLC, Coffeyville Resources, Nitrogen Fertilizers LLC, Ash Grove Cement Company, Kansas Ethanol LLC, Headwaters Clean Carbon Services, Black & Veatch, and Schlumberger Carbon Services, conducted a feasibility study of a large-scale CCS commercialization project that included large-scale CO{sub 2} sources. The overall objective of this project, entitled the 'Integrated Mid-Continent Carbon Capture, Sequestration and Enhanced Oil Recovery Project' was to design an integrated system of US mid-continent industrial CO{sub 2} sources with CO{sub 2} capture, and geologicmore » sequestration in deep saline formations and in oil field reservoirs with concomitant EOR. Findings of this project suggest that deep saline sequestration in the mid-continent region is not feasible without major financial incentives, such as tax credits or otherwise, that do not exist at this time. However, results of the analysis suggest that enhanced oil recovery with carbon sequestration is indeed feasible and practical for specific types of geologic settings in the Midwestern U.S.« less

  7. Black gold and blackmail: The politics of international oil coercion

    NASA Astrophysics Data System (ADS)

    Kelanic, Rosemary Ann

    Does oil hold potential as an instrument of international political coercion? Could a country use the threat of oil cutoff to blackmail other nations? If so, why, and under what circumstances is this likely to occur? I argue that oil coercion, under a narrow range of circumstances, offers great promise for a country to get its way in the international system. My argument consists of two main steps. First, I argue that oil is unique. No other strategic resource compares to petroleum when it comes to determining success on the battlefield. An adversary that can forcibly interdict the flow of oil to a belligerent in wartime could immobilize the target's military, essentially threatening the target with military defeat. The oil weapon is most potent when employed in this manner as a denial strategy of coercion. For a state to be at risk of oil coercion, it must have some degree of baseline vulnerability. I hypothesize that three variables determine a state's vulnerability to oil coercion. The first is its degree of dependence on foreign sources of oil. For oil coercion to be effective, the target must depend heavily on foreign sources of petroleum. Second, the target's geography matters greatly, because it affects a coercer's ability to block the flow of oil. Island states are particularly susceptible to military blockade. The third variable is the country's relative power, which determines whether or not the state can protect its supply lines. The second step of the argument explains why successful oil coercion is rare. I argue that states, as strategic actors, recognize the danger oil coercion poses to them. Consequently, they try to forestall coercion by taking anticipatory measures to safeguard their petroleum access before coercion can happen. States pursue four types of anticipatory strategies: internal measures, such as stockpiling; alliances with petroleum-producing countries; deterrence; and conquest of oil-producing regions. More often than not, these policies undermine the effectiveness of the oil weapon. As a result, when it comes to oil coercion, we see a paradox: coercive failures occur because states respond proactively to the potential of being coerced.

  8. Toxicity assessment of carbon black waste: A by-product from oil refineries.

    PubMed

    Zhen, Xu; Ng, Wei Cheng; Fendy; Tong, Yen Wah; Dai, Yanjun; Neoh, Koon Gee; Wang, Chi-Hwa

    2017-01-05

    In Singapore, approximately 30t/day of carbon-based solid waste are produced from petrochemical processes. This carbon black waste has been shown to possess physical properties that are characteristic of a good adsorbent such as high external surface area. Therefore, there is a growing interest to reutilize and process this carbon black waste into secondary materials such as adsorbents. However, the carbon black waste obtained from petrochemical industries may contain heavy metals that are hazardous to human health and the environment, hence restricting its full potential for re-utilization. Therefore, it is important to examine the possible toxicity effects and toxicity mechanism of carbon black waste on human health. In this study, inductively coupled plasma optical emission spectroscopy (ICP-OES) analysis showed that the heavy metals, vanadium (V), molybdenum (Mo) and nickel (Ni), were present in the carbon black waste in high concentrations. Three human cell lines (HepG2 cells, MRC-5 cells and MDA-MB-231 cells) were used to investigate the toxicity of carbon black waste extract in a variety of in vitro assays. Results from MTS assays indicated that carbon black waste extract decreased the viability of all three cell lines in a dose and time-dependent manner. Observations from confocal microscopy further confirmed this phenomenon. Flow cytometry assay also showed that carbon black waste extract induced apoptosis of human cell lines, and the level of apoptosis increased with increasing waste concentration. Results from reactive oxygen species (ROS) assay indicated that carbon black waste extract induced oxidative stress by increasing intracellular ROS generation in these three human cell lines. Moreover, induction of oxidative damage in these cells was also observed through the alteration of glutathione (GSH) and superoxide dismutase (SOD) activities. Last but not least, by treating the cells with V-spiked solution of concentration equivalent to that found in the carbon black waste extract, V was identified as the main culprit for the high toxicity of carbon black waste extract. These findings could potentially provide insight into the hazards of carbon black waste extract and its toxicity mechanism on human cell lines. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Impacts of chemicals on waterfowl reproduction and survival

    USGS Publications Warehouse

    White, D.H.; Stickel, L.F.

    1975-01-01

    Residues of organochlorine pesticides, PCB's, heavy metals, and other toxic chemicals are ubiquitous in the biosphere and are commonly found in tissues and eggs of wild birds. This paper reviews research on the effects of these chemicals, with particular reference to waterfowl. Extensive mortality of waterfowl has occurred in the Gulf Coast region as a result of ingestion of aldrin-treated rice seed. Populations of fulvous tree ducks (Dendrocygna bicolor) have declined in recent years in that area. DDE impaired reproduction of both mallards (Anas platyrhynchos) and black ducks (Arias rubripes) in experimental studies, resulting in thin shells, cracked eggs, and poor hatching success. Eggs incubated by the hens broke and cracked more frequently than those in incubators. In the field, residues are higher in ducks that feed on animal material than in vegetarians; differences are pronounced both geographically and among species. Mercury at levels that occur in certain heavily polluted areas in the United States and Canada has, in experimental studies, lowered the reproductive success of mallards and black ducks and reduced the survival of ducklings. Oil spills have killed many waterfowl as a result of oiled feathers and the intake of oil and more indirect physiological effects have been shown experimentally. Pollutants may affect waterfowl indirectly by changing the habitat and directly as a result of intake of toxic substances. They are unlikely to have been directly limiting factors in populations of such species as mallards. Their involvement with problem of other species, including canvasbacks (Aythya valisineria) and mergansers has still to be explored.

  10. Graphene sponge as an efficient and recyclable oil sorbent

    NASA Astrophysics Data System (ADS)

    Chen, Pin-Hsuan; Sie, Min-Chun; Jeng, Pei-Di; Wu, Ruei-Ci; Wang, Chen-Bin

    2017-09-01

    Fructose, an environmentally friendly reducing agent, was chosen during the process of reduction and self-assembly of graphene oxide (r-GO) via the hydrothermal method to prepare the graphene sponge (r-GS). Graphite oxide (GO) was prepared by oxidizing graphite (G) powders through a modified Hummers method. The GO dispersion (10 mg/mL) was mixed with equal mass ratio of fructose and ultrasonic-assisted dispersing in a beaker at RT for 30 min. Then, the suspended solution was transferred to a 200 mL Teflon-lined autoclave and maintained at 160 °C for 6 h. After cooling to RT, the black rod was washed with deionized water and lyophilized to obtain r-GS. The samples were characterized by XRD, TEM/SEM, BET, EA, FTIR, Raman and TPR. The absorption capacity and recycling measurement for oil over the fabricated r-GS was evaluated. In the preliminary results, the hydrophobic r-GS showed light weight and formed 3D porous structure that could enhance the absorption of organic solvents and oils. The absorption capacities of r-GS for various organic solvents and oils were quantified. The absorption capacities were in the range of 15 ˜ 38 g/g. The ultrahigh absorption capacity indicates that the absorption is more like an accommodation of oils in the hydrophobic micro- and macro-pores of the r-GS. In particular, the absorption capacity for vegetable oil is 37.8 g/g, indicating that the r-GS can be used for oil leakage treatment.

  11. Pyrolysis of waste tyres: a review.

    PubMed

    Williams, Paul T

    2013-08-01

    Approximately 1.5 billion tyres are produced each year which will eventually enter the waste stream representing a major potential waste and environmental problem. However, there is growing interest in pyrolysis as a technology to treat tyres to produce valuable oil, char and gas products. The most common reactors used are fixed-bed (batch), screw kiln, rotary kiln, vacuum and fluidised-bed. The key influence on the product yield, and gas and oil composition, is the type of reactor used which in turn determines the temperature and heating rate. Tyre pyrolysis oil is chemically very complex containing aliphatic, aromatic, hetero-atom and polar fractions. The fuel characteristics of the tyre oil shows that it is similar to a gas oil or light fuel oil and has been successfully combusted in test furnaces and engines. The main gases produced from the pyrolysis of waste tyres are H(2), C(1)-C(4) hydrocarbons, CO(2), CO and H(2)S. Upgrading tyre pyrolysis products to high value products has concentrated on char upgrading to higher quality carbon black and to activated carbon. The use of catalysts to upgrade the oil to a aromatic-rich chemical feedstock or the production of hydrogen from waste tyres has also been reported. Examples of commercial and semi-commercial scale tyre pyrolysis systems show that small scale batch reactors and continuous rotary kiln reactors have been developed to commercial scale. Copyright © 2013 Elsevier Ltd. All rights reserved.

  12. Thermolysis of scrap tire and rubber in sub/super-critical water.

    PubMed

    Li, Qinghai; Li, Fuxin; Meng, Aihong; Tan, Zhongchao; Zhang, Yanguo

    2018-01-01

    The rapid growth of waste tires has become a serious environmental issue. Energy and material recovery is regarded as a promising use for waste tires. Thermolysis of scrap tire (ST), natural rubber (NR), and styrene-butadiene rubber (SBR) was carried out in subcritical and supercritical water using a temperature-pressure independent adjustable batch tubular reactor. As a result, oil yields increased as temperature and pressure increased, and they reached maximum values as the state of water was near the critical point. However, further increases in water temperature and pressure reduced the oil yields. The maximum oil yield of 21.21% was obtained at 420 °C and 18 MPa with a reaction time of 40 min. The relative molecular weights of the chemicals in the oil products were in the range of 70-140 g/mole. The oil produced from ST, NR, and SBR contained similar chemical compounds, but the oil yield of SR was between those of NR and SBR. The oil yield from thermolysis of subcritical or supercritical water should be further improved. The main gaseous products, including CH 4 , C 2 H 2 , C 2 H 4 , C 2 H 6 , and C 3 H 8 , increased with reaction time, temperature, and pressure, whereas the solid residues, including carbon black and impurities, decreased. These results provide useful information to develop a sub/super-critical water thermolysis process for energy and material regeneration from waste tires. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Irradiation of Yarrowia lipolytica NRRL YB-567 creating novel strains with enhanced ammonia and oil production on protein and carbohydrate substrates.

    PubMed

    Lindquist, Mitch R; López-Núñez, Juan Carlos; Jones, Marjorie A; Cox, Elby J; Pinkelman, Rebecca J; Bang, Sookie S; Moser, Bryan R; Jackson, Michael A; Iten, Loren B; Kurtzman, Cletus P; Bischoff, Kenneth M; Liu, Siqing; Qureshi, Nasib; Tasaki, Kenneth; Rich, Joseph O; Cotta, Michael A; Saha, Badal C; Hughes, Stephen R

    2015-11-01

    Increased interest in sustainable production of renewable diesel and other valuable bioproducts is redoubling efforts to improve economic feasibility of microbial-based oil production. Yarrowia lipolytica is capable of employing a wide variety of substrates to produce oil and valuable co-products. We irradiated Y. lipolytica NRRL YB-567 with UV-C to enhance ammonia (for fertilizer) and lipid (for biodiesel) production on low-cost protein and carbohydrate substrates. The resulting strains were screened for ammonia and oil production using color intensity of indicators on plate assays. Seven mutant strains were selected (based on ammonia assay) and further evaluated for growth rate, ammonia and oil production, soluble protein content, and morphology when grown on liver infusion medium (without sugars), and for growth on various substrates. Strains were identified among these mutants that had a faster doubling time, produced higher maximum ammonia levels (enzyme assay) and more oil (Sudan Black assay), and had higher maximum soluble protein levels (Bradford assay) than wild type. When grown on plates with substrates of interest, all mutant strains showed similar results aerobically to wild-type strain. The mutant strain with the highest oil production and the fastest doubling time was evaluated on coffee waste medium. On this medium, the strain produced 0.12 g/L ammonia and 0.20 g/L 2-phenylethanol, a valuable fragrance/flavoring, in addition to acylglycerols (oil) containing predominantly C16 and C18 residues. These mutant strains will be investigated further for potential application in commercial biodiesel production.

  14. Evaluation of topical black seed oil in the treatment of allergic rhinitis.

    PubMed

    Alsamarai, Abdulghani Mohamed; Abdulsatar, Mohamed; Ahmed Alobaidi, Amina Hamed

    2014-03-01

    Allergic rhinitis (AR) is the most common manifestation of atopic reaction to inhaled allergens. It is a chronic inflammatory disease which may first appear at any age, but the onset is usually during childhood or adolescence. Up to date there is no curative treatment for this disorder and most of the drugs that were used for treatment only can induce symptomatic relief and some of them have side effect and can cause withdrawal symptoms. To evaluate the therapeutic efficacy of the Nigella sativa (NS) extract as treatment approach for allergic rhinitis. A total of 68 patients with AR were included in the study, of them 19 patients were with mild symptoms, 28 patients were with moderate symptoms and 21 patients were with severe symptoms. Each group was subdivided into active and control subgroups. To prove that the patient's symptoms were allergic in nature, skin test was performed for all patients. Any individual with negative skin test was excluded. The individuals in the active group received N. sativa oil and the control group individuals received ordinary food oil in the form of nasal drops for 6 weeks. After the 6 weeks treatment course, 100% of the patients in the mild active group became symptoms free; while in moderate active group 68.7% became symptoms free and 25% were improved; while in severe active group 58.3% became symptoms free and 25% were improved. In addition, 92.1% of total patients in the active group demonstrated improvement in their symptoms or were symptoms free, while the corresponding value was 30.1% in the control group (P=0.000). At the end of 6 weeks of treatment with topical use, the improvement in tolerability of allergen exposure in active group became 55.2% which was significant (P=0.006) as compared with control group which was accounted for 20% at the same time. Topical application of black seed oil was effective in the treatment of allergic rhinitis, with minimal side effects.

  15. [The effect of carbon tetrachloride poisoning on the activity of digestive proteases in rats and correction of the disorders with vegetable oils].

    PubMed

    Esaulenko, E E; Khil'chuk, M A; Bykov, I M

    2013-01-01

    The results of the study of activity of digestive proteases (pepsin, trypsin, chymotrypsin) in homogenates of stomach, pancreas and duodenum in experimental animals have been presented. Rats were exposed to intoxication with carbon tetrachloride (subcutaneous administration of a 50% oil solution of CCl4 in the dose of 0.5 ml per 100 g body weight) for three days and then they were given analysed oils (black nut, walnut and flax oil) intragastrically by gavage at a dose of 0.2 ml per day within 23 days. Pepsin level in gastric mucosa homogenates and chymotrypsin activity in pancreatic homogenates were determined by method of N.P. Pyatnitskiy based on on the ability of enzymes to coagulate dairy-acetate mixture, respectively, at 25 degrees C and 35 degrees C. Trypsin activity in homogenates of pancreatic was determined by method of Erlanger - Shaternikova colorimetrically. It has been established that intoxication with CCl4 decreased the synthesis of proteolytic enzymes of the stomach (by 51%) and pancreas (by 70-78%). Injections of analysed vegetable oils to animals contributed to the normalization of proteolytic enzymes synthesis. The conclusion that there are prospects of using the analysed vegetable oils containing large quantity of polyunsaturated fatty acids (omega-3 and omega-6) for the correction of detected biochemical abnormalities has been done.

  16. Assessment of Appalachian basin oil and gas resources: Utica-Lower Paleozoic Total Petroleum System: Chapter G.10 in Coal and petroleum resources in the Appalachian basin: distribution, geologic framework, and geochemical character

    USGS Publications Warehouse

    Ryder, Robert T.; Ruppert, Leslie F.; Ryder, Robert T.

    2014-01-01

    Both conventional oil and gas resources and continuous (unconventional) gas resources are present in the UticaLower Paleozoic TPS. Conventional oil and gas resources in the Utica-Lower Paleozoic TPS were assessed by the U.S. Geological Survey (USGS) in 2002 in the following assessment units (AU): (1) the Lower Paleozoic Carbonates in Thrust Belt AU, (2) the Knox Unconformity AU, (3) the Black River-Trenton Hydrothermal Dolomite AU, and (4) the Lockport Dolomite AU. The total estimated undiscovered oil and gas resources for these four AUs, at a mean value, was about 46 million barrels of oil (MMBO) and about 3 trillion cubic feet of gas (TCFG), respectively. In contrast, continuous (unconventional) gas resources in the TPS were assessed by the USGS in 2002 in four AUs associated with the “Clinton” sandstone, Medina sandstone, Medina Group sandstones, Tuscarora Sandstone, and sandstones in the Queenston Shale. The total estimated undiscovered gas for these four AUs, at a mean value, was about 26.8 TCFG. A hypothetical Utica Shale AU for oil(?) and continuous gas is identified in this report. In 2012, the Utica Shale was recognized by the USGS as a continuous AU and was assessed by Kirschbaum and others (2012).

  17. 33 CFR 151.13 - Special areas for Annex I of MARPOL 73/78.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... are the Mediterranean Sea area, the Baltic Sea area, the Black Sea area, the Red Sea area, the Gulfs... bilge water does not originate from cargo pump room bilges; (2) The bilge water is not mixed with oil... § 151.13 for the Red Sea area, Gulfs area, Gulf of Aden area, the Oman area of the Arabian Sea, and the...

  18. 33 CFR 151.13 - Special areas for Annex I of MARPOL 73/78.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... are the Mediterranean Sea area, the Baltic Sea area, the Black Sea area, the Red Sea area, the Gulfs... bilge water does not originate from cargo pump room bilges; (2) The bilge water is not mixed with oil... § 151.13 for the Red Sea area, Gulfs area, Gulf of Aden area, the Oman area of the Arabian Sea, and the...

  19. 33 CFR 151.13 - Special areas for Annex I of MARPOL 73/78.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... are the Mediterranean Sea area, the Baltic Sea area, the Black Sea area, the Red Sea area, the Gulfs... bilge water does not originate from cargo pump room bilges; (2) The bilge water is not mixed with oil... § 151.13 for the Red Sea area, Gulfs area, Gulf of Aden area, the Oman area of the Arabian Sea, and the...

  20. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Evans, R.D.; Lekia, S.D.L.

    This paper presents the results of parametric studies of two naturally fractured lenticular tight gas reservoirs, Fluvial E-1 and Puludal Zones 3 and 4, of the U.S. Department of Energy Multi-Well Experiment (MWX) site of Northwestern Colorado. The three-dimensional, two-phase, black oil reservoir simulator that was developed in a previous phase of this research program is also discussed and the capabilities further explored by applying it to several example problems.

  1. JPRS Report China.

    DTIC Science & Technology

    1990-01-31

    all time high. After 1985, however, problems mounted. Grain, edible oil, and cotton output faltered for five years running. What caused this turn...and market regulating exchange rates, the black market trade is running wild day by day. It has turned from underground to the open, and has become... wild - life and precious woody plant resources, arranges for and directs forest and wildlife species nature preserves and prevention and control of

  2. Geology and total petroleum systems of the Paradox Basin, Utah, Colorado, New Mexico, and Arizona

    USGS Publications Warehouse

    Whidden, Katherine J.; Lillis, Paul G.; Anna, Lawrence O.; Pearson, Krystal M.; Dubiel, Russell F.

    2014-01-01

    The most studied source intervals are the Pennsylvanian black shales that were deposited during relative high stands in an otherwise evaporitic basin. These black shales are the source for most of the discovered hydrocarbons in the Paradox Basin. A second oil type can be traced to either a Mississippian or Permian source rock to the west, and therefore requires long-distance migration to explain its presence in the basin. Upper Cretaceous continental to nearshore-marine sandstones are interbedded with coal beds that have recognized coalbed methane potential. Precambrian and Devonian TPSs are considered hypothetical, as both are known to have organic-rich intervals, but no discovered hydrocarbons have been definitively typed back to either of these units.

  3. Catalysts and process development for two-stage liquefaction. First quarterly report, January 1, 1992--March 31, 1992

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Cronauer, D.C.; Swanson, A.J.; Sajkowski, D.J.

    Research under way in this project centers upon developing and evaluating catalysts and process improvements for coal liquefaction in the two-stage, close-coupled catalytic process. The project is being carried out under contract to the United States Department of Energy. As discussed in the previous quarterly report, promising results were obtained by liquefying Illinois No. 6 bituminous and Black Thunder subbituminous coals using oil-soluble catalysts Molyvan L and molybdenum octoate. In this quarter, the liquefaction of Black Thunder coal was continued. Runs were made in catalytic/thermal (C/T) mode with supported AMOCAT{trademark} 1C (NiMo) and AMOCAT{trademark} 1B (Mo) catalysts. Although the initialmore » performance in these runs was good (90% conversion with no resid production), both catalysts deactivated rapidly. Spent catalysts showed severe coke deposition as well as formation of a calcium-rich shell on the catalyst surface. Overall, C/T liquefaction is not a good process option for Black Thunder coal.« less

  4. Operation of marine diesel engines on biogenic fuels: modification of emissions and resulting climate effects.

    PubMed

    Petzold, Andreas; Lauer, Peter; Fritsche, Uwe; Hasselbach, Jan; Lichtenstern, Michael; Schlager, Hans; Fleischer, Fritz

    2011-12-15

    The modification of emissions of climate-sensitive exhaust compounds such as CO(2), NO(x), hydrocarbons, and particulate matter from medium-speed marine diesel engines was studied for a set of fossil and biogenic fuels. Applied fossil fuels were the reference heavy fuel oil (HFO) and the low-sulfur marine gas oil (MGO); biogenic fuels were palm oil, soybean oil, sunflower oil, and animal fat. Greenhouse gas (GHG) emissions related to the production of biogenic fuels were treated by means of a fuel life cycle analysis which included land use changes associated with the growth of energy plants. Emissions of CO(2) and NO(x) per kWh were found to be similar for fossil fuels and biogenic fuels. PM mass emission was reduced to 10-15% of HFO emissions for all low-sulfur fuels including MGO as a fossil fuel. Black carbon emissions were reduced significantly to 13-30% of HFO. Changes in emissions were predominantly related to particulate sulfate, while differences between low-sulfur fossil fuels and low-sulfur biogenic fuels were of minor significance. GHG emissions from the biogenic fuel life cycle (FLC) depend crucially on energy plant production conditions and have the potential of shifting the overall GHG budget from positive to negative compared to fossil fuels.

  5. The diagnose of oil palm disease using Naive Bayes Method based on Expert System Technology

    NASA Astrophysics Data System (ADS)

    Nababan, Marlince; Laia, Yonata; Sitanggang, Delima; Sihombing, Oloan; Indra, Evta; Siregar, Saut; Purba, Windania; Mancur, Roy

    2018-04-01

    Expert system is dealt with system that used computer-based human intelligence to overcome particular problem which is commonly conducted by an expert. Frequent problem faced by the farmers of oil palm is the difficulty in defining the type of plant disease. As a result, the delay treatment of plant disease brings out the declining of farm products. An application system is needed to deal with the obstacles and diagnosing the type of oil palm plant disease. The researcher designed an intelligence-based application with input-output plan which is able to diagnose the type of oil palm plant disease by applying naive bayes method. Based on the research result by conducting bayes method with recognized symptom, diagnose of oil palm plant disease could be accomplished. The data of symptoms found are leaves turned yellow 0.4, dead leaves 0.4, black and brown color among the veins of leaves 0.5, young and old fruit with whole space 0.4, and decay of bunches is 0.3. The roots are tender in the amount of 0.5, and damage on sheath is 0.3. Through the chosen symptoms as mentioned above, the value of bayes is 80% with the type of disease is rotten bunch.

  6. An Oil-Bath-Based 293 K to 473 K Blackbody Source

    PubMed Central

    Fowler, Joel B.

    1996-01-01

    A high temperature oil-bath-based-black-body source has been designed and constructed in the Radiometric Physics Division at the National Institute of Standards and Technology, Gaithersburg, MD. The goal of this work was to design a large aperture blackbody source with highly uniform radiance across the aperture, good temporal stability, and good reproducibility. This blackbody source operates in the 293 K to 473 K range with blackbody temperature combined standard uncertainties of 7.2 mK to 30.9 mK. The calculated emissivity of this source is 0.9997 with a standard uncertainty of 0.0003. With a 50 mm limiting aperture at the cavity entrance, the emissivity increases to 0.99996. PMID:27805082

  7. Impact of winter cooling on the northern part of the Black Sea.

    NASA Astrophysics Data System (ADS)

    Savchenko, Anatolii

    2016-07-01

    Climate change in the future may have a negative impact on many countries due to the increasing surface temperature and sea level rise. Probably, unprecedented largest positive trend of surface temperature, which observed since the mid XX century, has associated with increasing human activities around the world. Moreover, this warming will continue in this century, and at the end of the XXI century will be 2 - 5 ºC. Thus, investigation and monitoring of current climate are very important and necessary tasks. Regional model data (downscaling) and satellite data are used, because of underdeveloped network of meteorological stations in the northern part of the Black Sea region. Experiment of downscaling was carried out for the Black Sea region with a high spatial resolution of 0.22° x 0.22° for 1958 - 2007(daily values). For the Black Sea were also used satellite data of sea surface temperature(SST) from MyOcean-2 Project, which CNR(Rome) has reprocessed Pathfinder V5.2 (PFV52) AVHRR data over period 1981 - 2012 with daily gap-free maps (L4) at the original PFV52 resolution at 0.04° x 0.04°. Correlation between satellite SST and surface temperature from regional model climate are amounted 0,99. Thus, surface temperature of model and satellite data for the Black Sea is much correlated between yourself. The following integral characteristics of the Black Sea are referred to the area of sea limited by the 44 - 47º N and 28 - 34º E. Maximum cooling of the north-western part of the Black Sea in winter is occurs after invasion of cold air across the northern border of the basin. In addition, this water area is also interesting in the presence of her huge oil and gas reserves, as well as the construction of liquefied gas (crude oil) terminals. The maximum values of total heat flux (sensible + latent heat fluxes= Q) corresponding to the minimum values of SST are observed during the periods of the negative phase of the NAO. Besides, fluxes with extreme days P (Q) = 95% (the number of which is 5% of the total number of winter days) contribute ≈ 16 - 18% of the total heat flux during the winter, and with P (Q) = 90% - approximately 30%. Typical synoptic situation of extreme winter cooling P (Q) = 95% is presence of anticyclone in the district of Carpathian Mountains. North-easterly flow of cold air at high velocities near-surface wind leads to extreme total heat flux and decreasing SST. Satellite images of clouds well illustrate such cases as, for example, cold air invasion to the Black Sea area on January 23, 2006 (Satellite TERRA). Because of increase of risk associated with climate change, this topic is particularly relevant for the marine area, which is subjected to strong weathering during extreme events in winter. The monitoring of this area will allow reducing the damage from extreme natural events in the future.

  8. Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles.

    PubMed

    Amooaghaie, Rayhaneh; Saeri, Mohammad Reza; Azizi, Morteza

    2015-10-01

    Despite the development potential in the field of nanotechnology, there is a concern about possible effects of nanoparticles on the environment and human health. In this study, silver nanoparticles (AgNPs) were synthesized by 'green' and 'chemical' methods. In the wet-chemistry method, sodium borohydrate, sodium citrate and silver nitrate were used as raw materials. Leaf extract of Nigella sativa was used as reducing as well as capping agent to reduce silver nitrate in the green synthesis method. In addition, toxic responses of both synthesized AgNPs were monitored on bone-building stem cells of mice as well as seed germination and seedling growth of six different plants (Lolium, wheat, bean and common vetch, lettuce and canola). In both synthesis methods, the colorless reaction mixtures turned brown and UV-visible spectra confirmed the presence of silver nanoparticles. Scanning electron microscope (SEM) observations revealed the predominance of silver nanosized crystallites and fourier transform infra-red spectroscopy (FTIR) indicated the role of different functional groups in the synthetic process. MTT assay showed cell viability of bone-building stem cells of mice was further in the green AgNPs synthesized using black cumin extract than chemical AgNPs. IC50 (inhibitory concentrations) values for seed germination, root and shoot length for 6 plants in green AgNPs exposures were higher than the chemical AgNPs. These results suggest that cytotoxicity and phytotoxicity of the green synthesized AgNPs were significantly less than wet-chemistry synthesized ones. This study indicated an economical, simple and efficient ecofriendly technique using leaves of N. sativa for synthesis of AgNPs and confirmed that green AgNPs are safer than chemically-synthesized AgNPs. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. Proapoptotic and Antimetastatic Properties of Supercritical CO2 Extract of Nigella sativa Linn. Against Breast Cancer Cells

    PubMed Central

    Baharetha, Hussein M.; Nassar, Zeyad D.; Aisha, Abdalrahim F.; Ahamed, Mohamed B. Khadeer; Al-Suede, Foaud Saleih R.; Kadir, Mohd Omar Abd; Ismail, Zhari

    2013-01-01

    Abstract Nigella sativa, commonly referred as black cumin, is a popular spice that has been used since the ancient Egyptians. It has traditionally been used for treatment of various human ailments ranging from fever to intestinal disturbances to cancer. This study investigated the apoptotic, antimetastatic, and anticancer activities of supercritical carbon dioxide (SC-CO2) extracts of the seeds of N. sativa Linn. against estrogen-dependent human breast cancer cells (MCF-7). Twelve extracts were prepared from N. sativa seeds using the SC-CO2 extraction method by varying pressure and temperature. Extracts were analyzed using FTIR and UV-Vis spectrometry. Cytotoxicity of the extracts was evaluated on various human cancer and normal cell lines. Of the 12 extracts, 1 extract (A3) that was prepared at 60°C and 2500 psi (∼17.24 MPa) showed selective antiproliferative activity against MCF-7 cells with an IC50 of 53.34±2.15 μg/mL. Induction of apoptosis was confirmed by evaluating caspases activities and observing the cells under a scanning electron microscope. In vitro antimetastatic properties of A3 were investigated by colony formation, cell migration, and cell invasion assays. The elevated levels of caspases in A3 treated MCF-7 cells suggest that A3 is proapoptotic. Further nuclear condensation and fragmentation studies confirmed that A3 induces cytotoxicity through the apoptosis pathway. A3 also demonstrated remarkable inhibition in migration and invasion assays of MCF-7 cells at subcytotoxic concentrations. Thus, this study highlights the therapeutic potentials of SC-CO2 extract of N. sativa in targeting breast cancer. PMID:24328702

  10. Effect of starter culture and turmeric on physico-chemical quality of carabeef pastirma.

    PubMed

    Maurya, P; Borpuzari, R N; Nath, D R; Nath, N C

    2010-01-01

    Carabeef samples were sliced, pressed, cured and divided into 6 groups. Starter cultures (Micrococcus varians M483 (MV), Staphylococcus carnosus (SC), Lactobacillus sakei (LS), M. varians M483+ Lb. sakei and Staph. carnosus + Lb. sakei) were inoculated at the dose of 10(6)-0(7)cfu/g and stored at 10 ± 1°C for 7 days. Uninoculated samples were maintained as control. Samples were then divided into 2 treatment groups. Samples of treatment 1 (T1) were smeared with a paste of turmeric followed by application of a thick layer of the paste of garlic, cumin, black pepper and red pepper whereas, samples of treatment 2 (T2) were applied with a thick layer of spices as above without turmeric. With the gradual fall in pH there was a reduction in water-holding capacity (WHC) of samples. The WHC of samples treated with SC+LS of T1 reduced to 6.3 ± 0.03 cm(2) and those inoculated with MV+LS of T2 to 6.2 ± 0.03 cm(2). The extract release volume (ERV) increased in all samples during storage. The least ERV of 11.7 and 11.6 ml were recorded in samples inoculated with MV of T1 and T2, respectively. The tyrosine (TV) and thiobarbituric acid (TBA) number of turmeric treated samples were significantly lower than non turmeric treated samples. The samples inoculated with LS had the least TV of 30.9 mg tyrosine/100 g of meat and TBA number of 0.06 mg manoladehyde/kg of meat. Samples inoculated with MV and LS of both T1 and T2 were better in physico-chemical qualities.

  11. Effect of an orally formulated processed black cumin, from Iranian traditional medicine pharmacopoeia, in relieving symptoms of knee osteoarthritis: A prospective, randomized, double-blind and placebo-controlled clinical trial.

    PubMed

    Salimzadeh, Ahmad; Ghourchian, Anahita; Choopani, Rasool; Hajimehdipoor, Homa; Kamalinejad, Mohammad; Abolhasani, Maryam

    2017-06-01

    Osteoarthritis is a global health problem, especially for the elderly. A good replacement for non-surgical treatments is the use of traditional medicines. We selected a revere plant (Nigella sativa L.), a widely utilized medicinal herb for the treatment of inflammatory conditions, from the Iranian traditional medicine (ITM) pharmacopoeia with proven anti-inflammatory and analgesic actions. We performed a prospective, randomized, double-blind, and placebo-controlled clinical trial, in order to investigate whether the herb is useful in alleviating the symptoms of knee osteoarthritis. American College of Rheumatology clinical criteria were the basis of diagnosis, while the Knee injury and Osteoarthritis Outcome Score (KOOS) questionnaire was considered as the main outcome measure. One hundred and ten eligible patients were assigned to receive a placebo or an active intervention (2 g/day of processed N. sativa seed powder in divided doses). Acetaminophen tablets were the rescue medicine. Finally, 40 patients in the placebo group and 37 patients in the active group completed the trial and were included in the statistical analysis. Both cohorts demonstrated statistically significant within-group differences (P < 0.05) in some subscales that were more prominent in the active group without any considerable adverse effects. Nevertheless, KOOS score results and the mean number of acetaminophen tablets used by patients showed no statistically significant between-group differences. It can be concluded that future programmed studies with larger sample sizes, longer follow-up periods, and other forms of N. sativa seeds as an active intervention is necessary to evaluate its efficacy in relieving the symptoms of knee osteoarthritis. © 2017 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Ltd.

  12. Thymoquinone Induces Cell Death in Human Squamous Carcinoma Cells via Caspase Activation-Dependent Apoptosis and LC3-II Activation-Dependent Autophagy

    PubMed Central

    Yu, Cheng-Chia; Lai, Yi-Yeh; Chen, Pei-Ni

    2014-01-01

    Background Thymoquinone (TQ), an active component of Nigella sativa or black cumin, elicits cytotoxic effects on various cancer cell lines. However, the anti-cancer effects of TQ on head and neck squamous cell carcinoma (HNSCC) remain unclear. Methodology/Principal Findings In this study, TQ elicited a strong cytotoxic effect on SASVO3, a highly malignant HNSCC cell line. The mechanisms of this cytotoxic effect were concentration dependent. TQ also induced apoptotic cell death in SASVO3 cells as indicated by an increase in Bax expression and caspase-9 activation. Apoptosis was possibly caspase-9 dependent because the exposure of cells to a caspase-9 inhibitor partially prevented cell death. The exposed cells also showed increased levels of autophagic vacuoles and LC3-II proteins, which are specific autophagy markers. Cell viability assay results further revealed that bafilomycin-A1, an autophagy inhibitor, enhanced TQ cytotoxicity; by comparison, Annexin V and propidium-iodide staining assay results showed that this inhibitor did not promote apoptosis. TQ treatment also increased the accumulation of autophagosomes. Using a lentivirus-shRNA system for LC3 silencing, we found that cell viability was eradicated in autophagy-defective cells. An in vivo BALB/c nude mouse xenograft model further showed that TQ administered by oral gavage reduced tumor growth via induced autophagy and apoptosis. Conclusions These findings indicated that TQ induced cell death in oral cancer cells via two distinct anti-neoplastic activities that can induce apoptosis and autophagy. Therefore, TQ is a promising candidate in phytochemical-based, mechanistic, and pathway-targeted cancer prevention strategies. PMID:25000169

  13. MEDSLIK oil spill model recent developments

    NASA Astrophysics Data System (ADS)

    Lardner, Robin; Zodiatis, George

    2016-04-01

    MEDSLIK oil spill model recent developments Robin Lardner and George Zodiatis Oceanography Center, University of Cyprus, 1678 Nicosia, Cyprus MEDSLIK is a well established 3D oil spill model that predicts the transport, fate and weathering of oil spills and is used by several response agencies and institutions around the Mediterranean, the Black seas and worldwide. MEDSLIK has been used operationally for real oil spill accidents and for preparedness in contingency planning within the framework of pilot projects with REMPEC-Regional Marine Pollution Emergency Response Centre for the Mediterranean Sea and EMSA-European Maritime Safety Agency. MEDSLIK has been implemented in many EU funded projects regarding oil spill predictions using the operational ocean forecasts, as for example the ECOOP, NEREIDs, RAOP-Med, EMODNET MedSea Check Point. Within the frame of MEDESS4MS project, MEDSLIK is at the heart of the MEDESS4MS multi model oil spill prediction system. The MEDSLIK oil spill model contains among other, the following features: a built-in database with 240 different oil types characteristics, assimilation of oil slick observations from in-situ or aerial, to correct the predictions, virtual deployment of oil booms and/or oil skimmers/dispersants, continuous or instantaneous oil spills from moving or drifting ships whose slicks merge can be modelled together, multiple oil spill predictions from different locations, backward simulations for tracking the source of oil spill pollution, integration with AIS data upon the availability of AIS data, sub-surface oil spills at any given water depth, coupling with SAR satellite data. The MEDSLIK can be used for operational intervention for any user-selected region in the world if the appropriate coastline, bathymetry and meteo-ocean forecast files are provided. MEDSLIK oil spill model has been extensively validated in the Mediterranean Sea, both in real oil spill incidents (i.e. during the Lebanese oil pollution crisis in summer 2006, the biggest oil pollution event in the Eastern Mediterranean so far) and through inter-comparison using drifters. The quality of the MEDSLIK oil spill model predictions depends on the quality of the meteo-ocean forecasting data that will be used. The guidelines set by the MEDESS4MS project to harmonize the meteo-ocean, oil spill and trajectory models input/output formats are implemented in MEDSLIK to suit the operational oil spill predictions. The output results of the trajectory predictions may available in the MEDESS4MS output standards (XML)and in ASCII, while the images in BMP or PNG, TIF,GIF, JPG (image), in KML (Google Earth).

  14. Black pepper (Piper nigrum) essential oil demonstrates tissue remodeling and metabolism modulating potential in human cells.

    PubMed

    Han, Xuesheng; Beaumont, Cody; Rodriguez, Damian; Bahr, Tyler

    2018-05-17

    Very few studies have investigated the biological activities of black pepper essential oil (BPEO) in human cells. Therefore, in the current study, we examined the biological activities of BPEO in cytokine-stimulated human dermal fibroblasts by analyzing the levels of 17 important protein biomarkers pertinent to inflammation and tissue remodeling. BPEO exhibited significant antiproliferative activity in these skin cells and significantly inhibited the production of Collagen I, Collagen III, and plasminogen activator inhibitor 1. In addition, we studied the effect of BPEO on the regulation of genome-wide expression and found that BPEO diversely modulated global gene expression. Further analysis showed that BPEO affected many important genes and signaling pathways closely related to metabolism, inflammation, tissue remodeling, and cancer signaling. This study is the first to provide evidence of the biological activities of BPEO in human dermal fibroblasts. The data suggest that BPEO possesses promising potential to modulate the biological processes of tissue remodeling, wound healing, and metabolism. Although further research is required, BPEO appears to be a good therapeutic candidate for a variety of health conditions including wound care and metabolic diseases. Research into the biological and pharmacological mechanisms of action of BPEO and its major active constituents is recommended. Copyright © 2018 John Wiley & Sons, Ltd.

  15. Increasing efficiency of TPP fuel suply system due to LNG usage as a reserve fuel

    NASA Astrophysics Data System (ADS)

    Zhigulina, E. V.; Khromchenkov, V. G.; Mischner, J.; Yavorovsky, Y. V.

    2017-11-01

    The paper is devoted to the analysis of fuel economy efficiency increase possibility at thermal power plants (TPP) due to the transition from the use of black oil as a reserve fuel to liquefied natural gas (LNG) produced at the very station. The work represents the technical solution that allows to generate, to store and to use LNG as the reserve fuel TPP. The annual amounts of black oil and natural gas that are needed to ensure the reliable operation of several power plants in Russia were assessed. Some original schemes of the liquefied natural gas production and storing as alternative reserve fuel generated by means of application of expansion turbines are proposed. The simulation results of the expansion process for two compositions of natural gas with different contents of high-boiling fractions are presented. The dependences of the condensation outlet and power generation from the flow initial parameters and from the natural gas composition are obtained and analysed. It was shown that the choice of a particular circuit design depends primarily on the specific natural gas composition. The calculations have proved the effectiveness and the technical ability to use liquefied natural gas as a backup fuel at reconstructed and newly designed gas power station.

  16. Effects of Surface-Engineered Nanoparticle-Based Dispersants for Marine Oil Spills on the Model Organism Artemia franciscana

    PubMed Central

    2015-01-01

    Fine particles are under active consideration as alternatives to chemical dispersants for large-scale petroleum spills. Fine carbon particles with engineered surface chemistry have been shown to stabilize oil-in-water emulsions, but the environmental impacts of large-scale particle introduction to the marine environment are unknown. Here we study the impact of surface-engineered carbon-black materials on brine shrimp (Artemia franciscana) as a model marine microcrustacean. Mortality was characterized at 50–1000 mg/L, and levels of heat shock protein 70 (hsp70) were characterized at sublethal particle concentrations (25–50 mg/L). Functionalized carbon black (CB) nanoparticles were found to be nontoxic at all concentrations, while hydrophobic (annealed) and as-produced CB induced adverse effects at high concentrations. CB was also shown to adsorb benzene, a model hydrocarbon representing the more soluble and toxic low-molecular weight aromatic fraction of petroleum, but the extent of adsorption was insufficient to mitigate benzene toxicity to Artemia in coexposure experiments. At lower benzene concentrations (25–75 mg/L), coexposure with annealed and as-produced CB increased hsp70 protein levels. This study suggests that surface functionalization for increased hydrophilicity can not only improve the performance of CB-based dispersants but also reduce their adverse environmental impacts on marine organisms. PMID:24823274

  17. Antibacterial Activity and Mechanism of Action of Black Pepper Essential Oil on Meat-Borne Escherichia coli

    PubMed Central

    Zhang, Jing; Ye, Ke-Ping; Zhang, Xin; Pan, Dao-Dong; Sun, Yang-Ying; Cao, Jin-Xuan

    2017-01-01

    The aim of this study was to investigate the antibacterial activity of black pepper essential oil (BPEO) on Escherichia coli, further evaluate the potential mechanism of action. Results showed that the minimum inhibition concentration (MIC) of BPEO was 1.0 μL/mL. The diameter of inhibition zone values were with range from 17.12 to 26.13 mm. 2 × MIC treatments had lower membrane potential and shorter kill-time than 1 × MIC, while control had the highest values. E. coli treated with BPEO became deformed, pitted, shriveled, adhesive, and broken. 2 × MIC exhibited the greatest electric conductivity at 1, 3, 5, 7, 9, 11, and 13 h, leaked DNA materials at 4, 8, 12, 16, 20, 24, and 28 h, proteins at 4, 6, 8, 10, 12, 14, and 16 h, potassium ion at 0, 0.5, 1, 1.5, and 2 h, phosphate ion at 0.5, 1, 1.5, and 2 h and ATP (P < 0.05); 1 × MIC had higher values than control. BPEO led to the leakage, disorder and death by breaking cell membrane. This study suggested that the BPEO has potential as the natural antibacterial agent in meat industry. PMID:28101081

  18. Sustained deposition of contaminants from the Deepwater Horizon spill.

    PubMed

    Yan, Beizhan; Passow, Uta; Chanton, Jeffrey P; Nöthig, Eva-Maria; Asper, Vernon; Sweet, Julia; Pitiranggon, Masha; Diercks, Arne; Pak, Dorothy

    2016-06-14

    The 2010 Deepwater Horizon oil spill resulted in 1.6-2.6 × 10(10) grams of petrocarbon accumulation on the seafloor. Data from a deep sediment trap, deployed 7.4 km SW of the well between August 2010 and October 2011, disclose that the sinking of spill-associated substances, mediated by marine particles, especially phytoplankton, continued at least 5 mo following the capping of the well. In August/September 2010, an exceptionally large diatom bloom sedimentation event coincided with elevated sinking rates of oil-derived hydrocarbons, black carbon, and two key components of drilling mud, barium and olefins. Barium remained in the water column for months and even entered pelagic food webs. Both saturated and polycyclic aromatic hydrocarbon source indicators corroborate a predominant contribution of crude oil to the sinking hydrocarbons. Cosedimentation with diatoms accumulated contaminants that were dispersed in the water column and transported them downward, where they were concentrated into the upper centimeters of the seafloor, potentially leading to sustained impact on benthic ecosystems.

  19. GC-MS characterisation and antibacterial activity evaluation of Nigella sativa oil against diverse strains of Salmonella.

    PubMed

    Sarwar, Arslan; Latif, Zakia

    2015-01-01

    Salmonella resistance is becoming a worldwide serious health issue in these days; therefore, it is an urgent need to develop some alternative approaches to overcome this problem. Twenty bacterial strains were isolated and purified from different environmental sources and confirmed as Salmonella by morphological and biochemical analyses. Further confirmation was done by 16s rRNA sequencing. Antibiotic susceptibility test was performed by well diffusion assay against different concentrations of Ceftriaxone and Ciprofloxacin. The behaviour of both antibiotics was different against diverse strains of Salmonella. Salmonella strains resistant to both antibiotics were analysed for antibacterial activity of natural extracts of Nigella sativa (black seeds). N. sativa oil was found to be more effective against Salmonella species for which even Ceftriaxone and Ciprofloxacin were ineffective. Gas chromatography and mass spectrometry analysis of N. sativa oil was also accomplished, exhibiting 10 compounds including thymoquinone, p-cymene, cis-carveol, thymol, α-phellandrene, α-pinene, β-pinene, trans-anethole, α-longipinene and longifolene.

  20. Oil Fires in Libya

    NASA Image and Video Library

    2016-01-28

    The oil refinery fires in Libya that were started by attacks on oil terminals in Libya in very early January continue. The stream of black smoke that emanates from the refinery has grown tremendously as the fires caused by the initial shelling have spread to giant storage tanks. These fires are reported to be raging in Sidra, on the coast between Sirte and Benghazi. Actively burning areas, detected by MODIS’s thermal bands, are outlined in red. The initial image of the fires taken on January 07, 2016 can be found here for comparison: www.nasa.gov/image-feature/goddard/2016/terra-captures-im... NASA image courtesy Jeff Schmaltz, MODIS Rapid Response Team NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

  1. Sustained deposition of contaminants from the Deepwater Horizon spill

    PubMed Central

    Yan, Beizhan; Passow, Uta; Chanton, Jeffrey P.; Nöthig, Eva-Maria; Asper, Vernon; Sweet, Julia; Pitiranggon, Masha; Diercks, Arne; Pak, Dorothy

    2016-01-01

    The 2010 Deepwater Horizon oil spill resulted in 1.6–2.6 × 1010 grams of petrocarbon accumulation on the seafloor. Data from a deep sediment trap, deployed 7.4 km SW of the well between August 2010 and October 2011, disclose that the sinking of spill-associated substances, mediated by marine particles, especially phytoplankton, continued at least 5 mo following the capping of the well. In August/September 2010, an exceptionally large diatom bloom sedimentation event coincided with elevated sinking rates of oil-derived hydrocarbons, black carbon, and two key components of drilling mud, barium and olefins. Barium remained in the water column for months and even entered pelagic food webs. Both saturated and polycyclic aromatic hydrocarbon source indicators corroborate a predominant contribution of crude oil to the sinking hydrocarbons. Cosedimentation with diatoms accumulated contaminants that were dispersed in the water column and transported them downward, where they were concentrated into the upper centimeters of the seafloor, potentially leading to sustained impact on benthic ecosystems. PMID:27247393

  2. Megacity Levels of Black and Brown Carbon in the Port of Valparaiso, Chile: A Toxic Mix of Bus, Truck, Ship, Industrial and Wood Burning Emissions

    NASA Astrophysics Data System (ADS)

    Marin, Julio; Raga, Graciela; Baumgardner, Darrel; Cordova, Ana; Arevalo, Jorge; Pozo, Diana

    2015-04-01

    Measurements of effective black carbon (eBC) have been made during three seasons (Winter, Spring and Summer) in Valparaiso, a coastal city that is located in the subtropics and is the largest commercial port in Chile. In addition to the ships in the harbor and the trucks that service the shipping industry, the primary public transport in the city is a bus system that uses diesel as its primary fuel source. Adding to the emissions of black and brown carbon (BC, BrC) from mobile sources is an oil refinery approximately 30 km to the north and in the winter many residences use wood burning as a primary source of heating. In winter the wind speeds are low, the boundary layer is shallow and there are frequent night time thermal inversions. The meteorology, coupled with a topography of very steep hillsides surrounding the bay, leads to episodes when the maximum eBC, measured with filter-based and photoacoustic techniques, often exceeds 10 µg m-3 and average mass concentrations are > 1.0 µg m-3. The absorption angstrom exponent (AAE), derived from measurements of the absorption coefficient at 550 nm and 870 nm, provides an indicator of the source of the eBC and brown carbon (BrC). The AAE ranges from 0 - 4, the lowest values, <1, associated with diesel emissions from public transport and the highest values, >3, with biomass combustion. The values in the mid-range appear to be associated with ship emissions or from the oil refinery. Removal of these aerosol particles is linked to the sea/land breeze circulations and periods of heavy fog and drizzle.

  3. Bimetallic iron and cobalt incorporated MFI/MCM-41 composite and its catalytic properties

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Li, Baoshan, E-mail: bsli@mail.buct.edu.cn; Xu, Junqing; Li, Xiao

    2012-05-15

    Graphical abstract: The formation of FeCo-MFI/MCM-41 composite is based on two steps, the first step of synthesizing the MFI-type proto-zeolite unites under hydrothermal conditions. The second step of assembling these zeolite fragment together new silica and heteroatom source on the CTAB surfactant micelle to synthesize the mesoporous product with hexagonal structure. Highlights: Black-Right-Pointing-Pointer Bimetallic iron and cobalt incorporated MFI/MCM-41 composite was prepared using templating method. Black-Right-Pointing-Pointer FeCo-MFI/MCM-41 composite simultaneously possessed two kinds of meso- and micro-porous structures. Black-Right-Pointing-Pointer Iron and cobalt ions incorporated into the silica framework with tetrahedral coordination. -- Abstract: The MFI/MCM-41 composite material with bimetallic Fe andmore » Co incorporation was prepared using templating method via a two-step hydrothermal crystallization procedure. The obtained products were characterized by a series of techniques including powder X-ray diffraction, N{sub 2} sorption, transmission electron microscopy, scanning electron microscope, H{sub 2} temperature programmed reduction, thermal analyses, and X-ray absorption fine structure spectroscopy of the Fe and Co K-edge. The catalytic properties of the products were investigated by residual oil hydrocracking reactions. Characterization results showed that the FeCo-MFI/MCM-41 composite simultaneously possessed two kinds of stable meso- and micro-porous structures. Iron and cobalt ions were incorporated into the silicon framework, which was confirmed by H{sub 2} temperature programmed reduction and X-ray absorption fine structure spectroscopy. This composite presented excellent activities in hydrocracking of residual oil, which was superior to the pure materials of silicate-1/MCM-41.« less

  4. Light induced degradation of phorbol esters.

    PubMed

    Yunping, Bu; Ha, Bui Thi Ngoc; Eunice, Yeo; Chueng, Lo Loong; Yan, Hong

    2012-10-01

    Jatropha curcas (Jatropha) is a tropical shrub that is gaining popularity as a biofuel feedstock plant. Phorbol esters (PEs) are tetracyclic tiglian diterpenoids that are present in Jatropha seeds and other parts of plant. Epidermal cell irritating and cancer promoting PEs not only reduce commercial values of Jatropha seed cake but also cause some safety and environment concerns on PE leaching to soil. A simple bioassay of PE toxicity was conducted by incubating 48 h old brine shrimp (Artemia salina) nauplii with Jatropha oil for 24 h. 1-4% of Jatropha oil (corresponding to PE concentration of 25-100 mg L(-1)) had mortality rate of 5-95%, with LC50 estimated to be 2.7% of oil or 67 mg L(-1) of PE. Jatropha oil was incubated with clay or black soil (autoclaved or non-autoclaved) in the darkness or under sunlight for different periods of time before oil was re-extracted and tested for PE content by HPLC and for remaining toxicity with the brine shrimp bioassay. Under sunlight, PE decreased to non-detectable level within six days. Toxicity reduced to less than 5% mortality rate that is comparable to rapeseed oil control within the same period. In contrast, PE level and toxicity remained little changed when Jatropha oil was incubated in the darkness. Such PE degradation/detoxification was also found independent of the presence of soil or soil microorganisms. We conclude that sunlight directly degrades and detoxifies PEs and this finding should alleviate the concern on long term environmental impact of PE leaching. Copyright © 2012 Elsevier Inc. All rights reserved.

  5. Energy Intensity and Greenhouse Gas Emissions from Oil Production in the Eagle Ford Shale

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yeh, Sonia; Ghandi, Abbas; Scanlon, Bridget R.

    A rapid increase in horizontal drilling and hydraulic fracturing in shale and “tight” formations that began around 2000 has resulted in record increases in oil and natural gas production in the U.S. This study examines energy consumption and greenhouse gas (GHG) emissions from crude oil and natural gas produced from ~8,200 wells in the Eagle Ford Shale in southern Texas from 2009 to 2013. Our system boundary includes processes from primary exploration wells to the refinery entrance gate (henceforth well-to-refinery or WTR). The Eagle Ford includes four distinct production zones—black oil (BO), volatile oil (VO), condensate (C), and dry gasmore » (G) zones—with average monthly gas-to-liquids ratios (thousand cubic feet per barrel—Mcf/bbl) varying from 0.91 in the BO zone to 13.9 in the G zone. Total energy consumed in drilling, extracting, processing, and operating an Eagle Ford well is ~1.5% of the energy content of the produced crude and gas in the BO and VO zones, compared with 2.2% in the C and G zones. On average, the WTR GHG emissions of gasoline, diesel, and jet fuel derived from crude oil produced in the BO and VO zones in the Eagle Ford play are 4.3, 5.0, and 5.1 gCO2e/MJ, respectively. Comparing with other known conventional and unconventional crude production where upstream GHG emissions are in the range 5.9–30 gCO2e/MJ, oil production in the Eagle Ford has lower WTR GHG emissions.« less

  6. Size distribution, chemical composition, and hygroscopicity of fine particles emitted from an oil-fired heating plant.

    PubMed

    Happonen, Matti; Mylläri, Fanni; Karjalainen, Panu; Frey, Anna; Saarikoski, Sanna; Carbone, Samara; Hillamo, Risto; Pirjola, Liisa; Häyrinen, Anna; Kytömäki, Jorma; Niemi, Jarkko V; Keskinen, Jorma; Rönkkö, Topi

    2013-12-17

    Heavy fuel oil (HFO) is a commonly used fuel in industrial heating and power generation and for large marine vessels. In this study, the fine particle emissions of a 47 MW oil-fired boiler were studied at 30 MW power and with three different fuels. The studied fuels were HFO, water emulsion of HFO, and water emulsion of HFO mixed with light fuel oil (LFO). With all the fuels, the boiler emitted considerable amounts of particles smaller than 200 nm in diameter. Further, these small particles were quite hygroscopic even as fresh and, in the case of HFO+LFO emulsion, the hygroscopic growth of the particles was dependent on particle size. The use of emulsions and the addition of LFO to the fuel had a reducing effect on the hygroscopic growth of particles. The use of emulsions lowered the sulfate content of the smallest particles but did not affect significantly the sulfate content of particles larger than 42 nm and, further, the addition of LFO considerably increased the black carbon content of particulate matter. The results indicate that even the fine particles emitted from HFO based combustion can have a significant effect on cloud formation, visibility, and air quality.

  7. Testing of advanced liquefaction concepts in HTI Run ALC-1: Coal cleaning and recycle solvent treatment

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Robbins, G.A.; Winschel, R.A.; Burke, F.P.

    In 1991, the Department of Energy initiated the Advanced Liquefaction Concepts Program to promote the development of new and emerging technology that has potential to reduce the cost of producing liquid fuels by direct coal liquefaction. Laboratory research performed by researchers at CAER, CONSOL, Sandia, and LDP Associates in Phase I is being developed further and tested at the bench scale at HTI. HTI Run ALC-1, conducted in the spring of 1996, was the first of four planned tests. In Run ALC-1, feed coal ash reduction (coal cleaning) by oil agglomeration, and recycle solvent quality improvement through dewaxing and hydrotreatmentmore » of the recycle distillate were evaluated. HTI`s bench liquefaction Run ALC-1 consisted of 25 days of operation. Major accomplishments were: 1) oil agglomeration reduced the ash content of Black Thunder Mine coal by 40%, from 5.5% to 3.3%; 2) excellent coal conversion of 98% was obtained with oil agglomerated coal, about 3% higher than the raw Black Thunder Mine coal, increasing the potential product yield by 2-3% on an MAF coal basis; 3) agglomerates were liquefied with no handling problems; 4) fresh catalyst make-up rate was decreased by 30%, with no apparent detrimental operating characteristics, both when agglomerates were fed and when raw coal was fed (with solvent dewaxing and hydrotreating); 5) recycle solvent treatment by dewaxing and hydrotreating was demonstrated, but steady-state operation was not achieved; and 6) there was some success in achieving extinction recycle of the heaviest liquid products. Performance data have not been finalized; they will be available for full evaluation in the new future.« less

  8. Isotope Ratio Mass Spectrometry and Shale Gas - What Is Possible with Current Technology?

    NASA Astrophysics Data System (ADS)

    Barrie, C. D.; Kasson, A.

    2014-12-01

    With ever increasing exploration and exploitation of 'unconventional' hydrocarbon resources, the drive to understand the origins, history and importance of these resources and their effects on the surrounding environment (i.e. ground waters) has never been more important. High-throughput, high-precision isotopic measurements are therefore a key tool in this industry to both understand the gas generated and monitor the development and stability of wells through time. With the advent of cavity ringdown spectroscopy (CRDS) instrumentation, there has been a push in some applications - environmental & atmospheric - to gather more and more data directly at the location of collection or at dedicated field stations. Furthermore, CRDS has resulted in users seeking greater autonomy of instrumentation and so-called black box technology. Traditionally IRMS technology has not met any of these demands, requiring very specific and extensive footprint, power and environmental requirements. This has meant that the 'Oil & Gas' sector, which for natural gases measurements requires GC-IRMS technology - not possible via CRDS - loses time, money and manpower as samples get sent to central facility or contract labs with potentially long lee times. However, recent developments in technology mean that IRMS systems exist which are benchtop, have much lower power requirements, standard power connections and as long as housed in a temperature controlled field stations can be deployed anywhere. Furthermore, with advances in electronics and software IRMS systems are approaching the black box level of newer instrumentation while maintaining the flexibility and abilities of isotope ratio mass spectrometry. This presentation will outline changes in IRMS technology applicable to the Oil & Gas industry, discuss the feasibility of true 'field' deployability and present results from a range of Oil & Gas samples.

  9. γ-Oryzanol-Rich Black Rice Bran Extract Enhances the Innate Immune Response.

    PubMed

    Shin, Soon Young; Kim, Heon-Woong; Jang, Hwan-Hee; Hwang, Yu-Jin; Choe, Jeong-Sook; Lim, Yoongho; Kim, Jung-Bong; Lee, Young Han

    2017-09-01

    The innate immune response is an important host primary defense system against pathogens. γ-Oryzanol is one of the nutritionally important phytoceutical components in rice bran oil. The goal of this study was to investigate the effect of γ-oryzanol-rich extract from black rice bran (γORE) on the activation of the innate immune system. In this study, we show that γORE increased the expression of CD14 and Toll-like receptor 4 and enhanced the phagocytic activity of RAW264.7 macrophages. Furthermore, γORE and its active ingredient γ-oryzanol promoted the secretion of innate cytokines, interleukin-8, and CCL2, which facilitate phagocytosis by RAW264.7 cells. These findings suggest that γ-oryzanol in the γORE enhances innate immune responses.

  10. Relative Condition Factors of Fish as Bioindicators One Year after the Deepwater Horizon Oil Spill

    DTIC Science & Technology

    2012-02-01

    parts of the water column, and they have different but somewhat overlapping feeding habits . Black drum eat at the seafloor and prefer shellfish like...oysters and bottom-dwelling crustaceans like crab (Matlock 1990). Red drum feed throughout the water column and prefer to eat shrimp, smaller fish... Feeding habits of red drum (Sciaenops ocellatus) in Galveston Bay, Texas: Seasonal diet variation and predator- prey size relationships. Estuaries and

  11. Use of Heavy Lift Ships as Modular Casualty Receiving Ships

    DTIC Science & Technology

    2007-04-01

    ship. • Combination product tanker and heavy lift ship. • Specialist dock or yacht transport ship. The M.V. Black Marlin, CombiDock, and the...regulations. • Pollution must be disposed of properly as set by various organizations. Pollutants include oil, noxious liquid substances in bulk, sewage ...pictured in Figure 21, is typically used to ship yachts from one location to another, and yacht owners have the option to travel with their yachts

  12. Biological production of products from waste gases

    DOEpatents

    Gaddy, James L.

    2002-01-22

    A method and apparatus are designed for converting waste gases from industrial processes such as oil refining, and carbon black, coke, ammonia, and methanol production, into useful products. The method includes introducing the waste gases into a bioreactor where they are fermented to various products, such as organic acids, alcohols, hydrogen, single cell protein, and salts of organic acids by anaerobic bacteria within the bioreactor. These valuable end products are then recovered, separated and purified.

  13. In-vitro antimicrobial activity and identification of bioactive components using GC-MS of commercially available essential oils in Saudi Arabia.

    PubMed

    Ashraf, Syed Amir; Al-Shammari, Eyad; Hussain, Talib; Tajuddin, Shaikh; Panda, Bibhu Prasad

    2017-11-01

    This study was designed to evaluate antimicrobial activity and chemical composition of four different plant essential oils i.e. Ginger oil (GiO), Black seed oil (BSO), Oregano oil (OO) and Rose oil (RO) against different bacterial and fungal strains. Anti-microbial activities of selected essential oils were determined by the microbiological technique using Agar well diffusion assay. After in vitro study, most of the essential oils showed antimicrobial activity against all the selected pathogens. Among all the tested oils, GiO showed strong antimicrobial activity. GiO showed highest antimicrobial activity against Shigella (119.79%), Enteococcus hirae (110.61%) and Escherichia coli (106.02%), when compared with the tetracycline (50 µg/mL) activity. However, Antifungal activity of GiO was found to be present against Candida albicans and Aspergilluas flavus , when compared with clotrimazole (50 µg/mL) activity. Among all the selected bacteria, BSO showed maximum antimicrobial activity against the E. coli followed by Citrobacter freundii. Moreover, BSO had highest zone of inhibition against the C. ablicans (33.58%). OO indicated that, Shigella had the highest sensitivity (12.6 ± 0.58, 131.25%), followed by E. hirae (19.1 ± 0.61, 96.46%) and Salmonella typhi (15.2 ± 0.27, 83.06%) when compared with tetracycline activity. OO showed poor sensitivity against all the selected fungal strains. Furthermore, Gas Chromatography analysis revealed that, Gingerol (10.86%) was the chief chemical constituents found in GiO followed by α -Sesquiphellandrene (6.29%), Zingiberene (5.88%). While, BSO, OO and RO had higher percentage of p-Cymene (6.90%), Carvacrol (15.87%) and Citronellol (8.07%) respectively. The results exhibited that the essential oils used for this study was the richest source for antimicrobial activity which indicates the presence of broad spectrum antimicrobial compounds in these essential oils. Hence, essential oils and their components can be recommended for therapeutic purposes as source of an alternative medicine.

  14. Formation of the metal and energy-carrier price clusters on the world market of nonferrous metals in the postcrisis period

    NASA Astrophysics Data System (ADS)

    Bogdanov, S. V.; Shevelev, I. M.; Chernyi, S. A.

    2016-06-01

    The laws of formation of price clusters are revealed upon statistical processing of the data on changing the quotation prices of nonferrous and precious metals, oil, black oil, gasoline, and natural gas in the postcrisis period from January 1, 2009 to November 1, 2013. It is found that the metal prices entering in the price cluster of nonferrous metals most strongly affect the formation of the nonferrous metal price and that the prices of precious metals and energy carriers correct the exchange price of the metal to some extent but do not determine its formation. Equations are derived to calculate the prices. The results of calculation by these equations agree well with the real nonferrous metal prices in the near future.

  15. Inhibitory effect of essential oils against Trichosporon ovoides causing Piedra Hair Infection.

    PubMed

    Saxena, Seema; Uniyal, Veena; Bhatt, R P

    2012-10-01

    Piedra, is an asymptomatic fungal infection of the hair shaft, resulting in the formation of nodules of different hardness on the infected hair. The infection also known as Trichomycosis nodularis is a superficial fungal infection arising from the pathogen being restricted to the stratum corneum with little or no tissue reaction. The nodules are a concretion of hyphae and fruiting bodies of the fungus. Two varieties of Piedra may be seen, Black Piedra and White Piedra. The fungus Trichosporon ovoides is involved in the occurrence of both types of Piedras. The purpose of this study was to examine the effectiveness of selected essential oils for the control of growth of the fungus and to determine whether the antifungal effect was due to the major compounds of the oils. Two screening methods viz. Agar well diffusion assay and Minimum Inhibitory Concentration were adopted for the study. MIC and MFC were determined by tube dilution method. Essential oils from Eucalyptus, Ocimum basilicum, Mentha piperita, Cymbopogon flexuosus, Cymbopogon winterians, Trachyspermum ammi, Zingiber officinalis, Citrus limon, Cinnamomon zeylanicum, Salvia sclarea, Citrus aurantifolia, Melaleuca alternifolia, Citrus aurantium, Citrus bergamia, Pogostemon pathchouli, Cedrus atlantica, Jasminum officinale, Juniperus communis, Abelmoschus moschatus, Cyperus scariosus, Palargonium graveolens, Boswellia carterii, Rosa damascene, Veteveria zizanoides and Commiphora myrrha were evaluated. The essential oils of Cymbopogon winterians, Mentha piperita, Cinnamomum zeylanicum, Melaleuca alternifolia and Eucalyptus globulus were proved to be most effective against the fungus Trichosporon ovoides.

  16. Diethyl hexyl phthalate-induced changes in insulin signaling molecules and the protective role of antioxidant vitamins in gastrocnemius muscle of adult male rat

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Srinivasan, Chinnapaiyan; Khan, Adam Ismail; Balaji, Venkataraman

    Diethyl hexyl phthalate (DEHP) is an endocrine disruptor, it influences various organ systems in human beings and experimental animals. DEHP reduced the serum testosterone and increased the blood glucose, estradiol, T{sub 3} and T{sub 4} in rats. However, the effect of DEHP on insulin signaling and glucose oxidation in skeletal muscle is not known. Adult male albino rats were divided into four groups: Group I: Control; Groups II and III: DEHP treated (dissolved in olive oil at a dose of 10 and 100 mg/kg body weight, respectively, once daily through gastric intubation for 30 days); and Group IV: DEHP (100more » mg/kg body weight) plus vitamins E (50 mg/kg body weight) and C (100 mg/kg body weight) dissolved in olive oil and distilled water, respectively, once daily through gastric intubation for 30 days. On completion of treatment, animals were euthanized and perfused (whole body); gastrocnemius muscle was dissected out and subjected to assessment of various parameters. DEHP treatment increased the H{sub 2}O{sub 2}, hydroxyl radical levels and lipid peroxidation which disrupt the membrane integrity and insulin receptor. DEHP impaired the insulin signal transduction, glucose uptake and oxidation through decreased expression of plasma membrane GLUT4, which may partly be responsible for the elevation of fasting blood glucose level. The present study suggests that DEHP exposure affects glucose oxidation in skeletal muscle and is mediated through enhanced lipid peroxidation, impaired insulin signaling and GLUT4 expression in plasma membrane. Antioxidant vitamins (C and E) have a protective role against the adverse effect of DEHP. -- Highlights: Black-Right-Pointing-Pointer DEHP treatment significantly decreased serum insulin and testosterone levels. Black-Right-Pointing-Pointer Increased ROS and decreased glucose uptake were observed in DEHP treated animals. Black-Right-Pointing-Pointer Impaired insulin signaling in gastrocnemius muscle was observed in DEHP treatment. Black-Right-Pointing-Pointer Vitamins C and E alter ROS, glucose uptake, oxidation and insulin signaling molecules.« less

  17. ENVIRONMENTAL BASELINE SURVEY REPORT FOR WEST BLACK OAK RIDGE, EAST BLACK OAK RIDGE, MCKINNEY RIDGE, WEST PINE RIDGE, AND PARCEL 21D IN THE VICINITY OF THE EAST TENNESSEE TECHNOLOGY PARK, OAK RIDGE, TENNESSEE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    David A. King

    2012-11-29

    This environmental baseline survey (EBS) report documents the baseline environmental conditions of five land parcels located near the U.S. Department of Energy’s (DOE’s) East Tennessee Technology Park (ETTP), including West Black Oak Ridge, East Black Oak Ridge, McKinney Ridge, West Pine Ridge, and Parcel 21d. The goal is to obtain all media no-further-investigation (NFI) determinations for the subject parcels considering existing soils. To augment the existing soils-only NFI determinations, samples of groundwater, surface water, soil, and sediment were collected to support all media NFI decisions. The only updates presented here are those that were made after the original issuance ofmore » the NFI documents. In the subject parcel where the soils NFI determination was not completed for approval (Parcel 21d), the full process has been performed to address the soils as well. Preparation of this report included the detailed search of federal government records, title documents, aerial photos that may reflect prior uses, and visual inspections of the property and adjacent properties. Interviews with current employees involved in, or familiar with, operations on the real property were also conducted to identify any areas on the property where hazardous substances and petroleum products, or their derivatives, and acutely hazardous wastes may have been released or disposed. In addition, a search was made of reasonably obtainable federal, state, and local government records of each adjacent facility where there has been a release of any hazardous substance or any petroleum product or their derivatives, including aviation fuel and motor oil, and which is likely to cause or contribute to a release of any hazardous substance or any petroleum product or its derivatives, including aviation fuel or motor oil, on the real property. A radiological survey and soil/sediment sampling was conducted to assess baseline conditions of Parcel 21d that were not addressed by the soils-only NFI reports. Groundwater sampling was also conducted to support a Parcel 21d decision.« less

  18. A Black Phosphate Conversion Coating on Steel Surface Using Antimony(III)-Tartrate as an Additive

    NASA Astrophysics Data System (ADS)

    Li, Feng; Wang, Guiping

    2016-05-01

    A novel black phosphate conversion coating was formed on steel surface through a Zn-Mn phosphating bath containing mainly ZnO, H3PO4, Mn(H2PO4)2, and Ca(NO3)2, where antimony(III)-tartrate was used as the blackening agent of phosphatization. The surface morphology and composition of the coating were characterized by scanning electron microscopy, energy dispersion spectroscopy, and x-ray photoelectron spectroscopy. Corrosion resistance of the coating was studied by potentiodynamic polarization curves and electrochemical impedance spectroscopy. The pH value of the solution had significant influence on the formation and corrosion resistance of the coating. The experimental results indicated that the Sb plays a vital role in the blackening of phosphate conversion coating. The optimal concentration of antimony(III)-tartrate in the phosphating bath used in this experiment was 1.0 g L-1, as higher values reduced the corrosion resistance of the coating. In addition, by saponification and oil seals, the corrosion duration of the black phosphate coating in a copper sulfate spot test can be as long as 20 min.

  19. Characterisation of fatty acid, carotenoid, tocopherol/tocotrienol compositions and antioxidant activities in seeds of three Chenopodium quinoa Willd. genotypes.

    PubMed

    Tang, Yao; Li, Xihong; Chen, Peter X; Zhang, Bing; Hernandez, Marta; Zhang, Hua; Marcone, Massimo F; Liu, Ronghua; Tsao, Rong

    2015-05-01

    Composition of fatty acids, tocopherols, tocotrienols, and carotenoids, and their contribution to antioxidant activities were investigated in seeds of three coloured quinoa cultivars (white, red and black). The major components and individual compounds were significantly different, and their concentrations were higher in darker seeds (p < 0.05). The oil yield was 6.58-7.17% which contained predominantly unsaturated fatty acids (89.42%). The ratio of omega-6/omega-3 fatty acid was ca. 6/1. The total tocopherol content ranged from 37.49 to 59.82 μg/g and mainly consisted of γ-tocopherol. Trace amount of α- and β-tocotrienols was also found. Black quinoa had the highest vitamin E followed by red and white quinoas. Carotenoids, mainly trans-lutein (84.7-85.6%) and zeaxanthin were confirmed for the first time in quinoa seeds, and the concentration was also the highest in black seeds. The antioxidant activities of lipophilic extracts were positively correlated with polyunsaturated fatty acids, total carotenoids and total tocopherols. Copyright © 2014. Published by Elsevier Ltd.

  20. Pyrolysis of waste tyres: A review

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Williams, Paul T., E-mail: p.t.williams@leeds.ac.uk

    2013-08-15

    Graphical abstract: - Highlights: • Pyrolysis of waste tyres produces oil, gas and char, and recovered steel. • Batch, screw kiln, rotary kiln, vacuum and fluidised-bed are main reactor types. • Product yields are influenced by reactor type, temperature and heating rate. • Pyrolysis oils are complex and can be used as chemical feedstock or fuel. • Research into higher value products from the tyre pyrolysis process is reviewed. - Abstract: Approximately 1.5 billion tyres are produced each year which will eventually enter the waste stream representing a major potential waste and environmental problem. However, there is growing interest inmore » pyrolysis as a technology to treat tyres to produce valuable oil, char and gas products. The most common reactors used are fixed-bed (batch), screw kiln, rotary kiln, vacuum and fluidised-bed. The key influence on the product yield, and gas and oil composition, is the type of reactor used which in turn determines the temperature and heating rate. Tyre pyrolysis oil is chemically very complex containing aliphatic, aromatic, hetero-atom and polar fractions. The fuel characteristics of the tyre oil shows that it is similar to a gas oil or light fuel oil and has been successfully combusted in test furnaces and engines. The main gases produced from the pyrolysis of waste tyres are H{sub 2}, C{sub 1}–C{sub 4} hydrocarbons, CO{sub 2}, CO and H{sub 2}S. Upgrading tyre pyrolysis products to high value products has concentrated on char upgrading to higher quality carbon black and to activated carbon. The use of catalysts to upgrade the oil to a aromatic-rich chemical feedstock or the production of hydrogen from waste tyres has also been reported. Examples of commercial and semi-commercial scale tyre pyrolysis systems show that small scale batch reactors and continuous rotary kiln reactors have been developed to commercial scale.« less

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