Internalisation of the bleomycin molecules responsible for bleomycin toxicity: a receptor-mediated endocytosis mechanism.

PubMed

Pron, G; Mahrour, N; Orlowski, S; Tounekti, O; Poddevin, B; Belehradek, J; Mir, L M

1999-01-01

Bleomycin (BLM) does not diffuse through the plasma membrane but nevertheless displays cytotoxic activity due to DNA break generation. The aim of the study was to describe the mechanism of BLM internalisation. We previously provided evidence for the existence of BLM-binding sites at the surface of DC-3F Chinese hamster fibroblasts, as well as of their involvement in BLM cytotoxicity on DC-3F cells and related BLM-resistant sublines. Here we report that A253 human cells and their BLM-resistant subline C-10E also possessed a membrane protein of ca. 250 kDa specifically binding BLM. Part of this C-10E cell resistance could be explained by a decrease in the number of BLM-binding sites exposed at the cell surface with respect to A253 cells. The comparison between A253 and DC-3F cells exposing a similar number of BLM-binding sites revealed that the faster the fluid phase endocytosis, the greater the cell sensitivity to BLM. Moreover, the experimental modification of endocytotic vesicle size showed that BLM cytotoxicity was directly correlated with the flux of plasma membrane area engulfed during endocytosis rather than with the fluid phase volume incorporated. Thus, BLM would be internalised by a receptor-mediated endocytosis mechanism which would first require BLM binding to its membrane receptor and then the transfer of the complex into intracellular endocytotic vesicles, followed by BLM entry into the cytosol, probably from a nonacidic compartment.

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2012 CFR

2012-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Will BLM prorate annual maintenance or oil...

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2014 CFR

2014-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Will BLM prorate annual maintenance or oil...

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2013 CFR

2013-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Will BLM prorate annual maintenance or oil...

43 CFR 3834.13 - Will BLM prorate annual maintenance or oil shale fees?

Code of Federal Regulations, 2011 CFR

2011-10-01

... FOR MINING CLAIMS OR SITES Fee Payment § 3834.13 Will BLM prorate annual maintenance or oil shale fees? BLM will not prorate annual maintenance or oil shale fees if you hold a mining claim or site for only... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Will BLM prorate annual maintenance or oil...

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

43 CFR 2806.32 - How does BLM determine the population strata served?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM determine the population... does BLM determine the population strata served? (a) BLM determines the population strata served as follows: (1) If the site or facility is within a designated RMA, BLM will use the population strata of the...

Dynamics of bleomycin interaction with a strongly bound hairpin DNA substrate, and implications for cleavage of the bound DNA.

PubMed

Bozeman, Trevor C; Nanjunda, Rupesh; Tang, Chenhong; Liu, Yang; Segerman, Zachary J; Zaleski, Paul A; Wilson, W David; Hecht, Sidney M

2012-10-31

Recent studies involving DNAs bound strongly by bleomycins have documented that such DNAs are degraded by the antitumor antibiotic with characteristics different from those observed when studying the cleavage of randomly chosen DNAs in the presence of excess Fe·BLM. In the present study, surface plasmon resonance has been used to characterize the dynamics of BLM B(2) binding to a strongly bound hairpin DNA, to define the effects of Fe(3+), salt, and temperature on BLM-DNA interaction. One strong primary DNA binding site, and at least one much weaker site, were documented. In contrast, more than one strong cleavage site was found, an observation also made for two other hairpin DNAs. Evidence is presented for BLM equilibration between the stronger and weaker binding sites in a way that renders BLM unavailable to other, less strongly bound DNAs. Thus, enhanced binding to a given site does not necessarily result in increased DNA degradation at that site; i.e., for strongly bound DNAs, the facility of DNA cleavage must involve other parameters in addition to the intrinsic rate of C-4' H atom abstraction from DNA sugars.

Characterization of Bleomycin-Mediated Cleavage of a Hairpin DNA Library

PubMed Central

Segerman, Zachary J.; Roy, Basab; Hecht, Sidney M.

2013-01-01

A study of BLM A5 was conducted using a previously isolated library of hairpin DNAs found to bind strongly to metal free BLM. The ability of Fe(II)•BLM to effect cleavage on both the 3' and 5'-arms of the hairpin DNAs was characterized. The strongly bound DNAs were found to be efficient substrates for Fe•BLM A5-mediated hairpin DNA cleavage. Surprisingly, the most prevalent site of BLM-mediated cleavage was found to be the 5′-AT-3′ dinucleotide sequence. This dinucleotide sequence, and other sequences generally not cleaved well by BLM when examined using arbitrarily chosen DNA substrates, were apparent when examining the library of ten hairpin DNAs. In total, 132 sites of DNA cleavage were produced by exposure of the hairpin DNA library to Fe•BLM A5. The existence of multiple sites of cleavage on both the 3′- and 5′-arms of the hairpin DNAs suggested that some of these might be double-strand cleavage events. Accordingly, an assay was developed with which to test the propensity of the hairpin DNAs to undergo double-strand DNA damage. One hairpin DNA was characterized using this method, and gave results consistent with earlier reports of double-strand DNA cleavage, but with a sequence selectivity different from those reported previously. PMID:23834496

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kilpatrick, Laura E.; Cotter, Ed

The U.S. Department of Energy (DOE) Office of Legacy Management is responsible for administering the DOE Uranium Leasing Program (ULP) and its 31 uranium lease tracts located in the Uravan Mineral Belt of southwestern Colorado (see Figure 1). In addition to administering the ULP for the last six decades, DOE has also undertaken the significant task of reclaiming a large number of abandoned uranium (legacy) mine sites and associated features located throughout the Uravan Mineral Belt. In 1995, DOE initiated a 3-year reconnaissance program to locate and delineate (through extensive on-the-ground mapping) the legacy mine sites and associated features containedmore » within the historically defined boundaries of its uranium lease tracts. During that same time frame, DOE recognized the lack of regulations pertaining to the reclamation of legacy mine sites and contacted the U.S. Bureau of Land Management (BLM) concerning the reclamation of legacy mine sites. In November 1995, The BLM Colorado State Office formally issued the United States Department of the Interior, Colorado Bureau of Land Management, Closure/Reclamation Guidelines, Abandoned Uranium Mine Sites as a supplement to its Solid Minerals Reclamation Handbook (H-3042-1). Over the next five-and-one-half years, DOE reclaimed the 161 legacy mine sites that had been identified on DOE withdrawn lands. By the late 1990's, the various BLM field offices in southwestern Colorado began to recognize DOE's experience and expertise in reclaiming legacy mine sites. During the ensuing 8 years, BLM funded DOE (through a series of task orders) to perform reclamation activities at 182 BLM mine sites. To date, DOE has reclaimed 372 separate and distinct legacy mine sites. During this process, DOE has learned many lessons and is willing to share those lessons with others in the reclamation industry because there are still many legacy mine sites not yet reclaimed. DOE currently administers 31 lease tracts (11,017 ha) that collectively contain over 220 legacy (abandoned) uranium mine sites. This contrasts to the millions of hectares administered by the BLM, the U.S. Forest Service, and other federal, tribal, and state agencies that contain thousands of such sites. DOE believes that the processes it has used provide a practical and cost-effective approach to abandoned uranium mine-site reclamation. Although the Federal Acquisition Regulations preclude DOE from competing with private industry, DOE is available to assist other governmental and tribal agencies in their reclamation efforts. (authors)« less

Bloom DNA Helicase Facilitates Homologous Recombination between Diverged Homologous Sequences*

PubMed Central

Kikuchi, Koji; Abdel-Aziz, H. Ismail; Taniguchi, Yoshihito; Yamazoe, Mitsuyoshi; Takeda, Shunichi; Hirota, Kouji

2009-01-01

Bloom syndrome caused by inactivation of the Bloom DNA helicase (Blm) is characterized by increases in the level of sister chromatid exchange, homologous recombination (HR) associated with cross-over. It is therefore believed that Blm works as an anti-recombinase. Meanwhile, in Drosophila, DmBlm is required specifically to promote the synthesis-dependent strand anneal (SDSA), a type of HR not associating with cross-over. However, conservation of Blm function in SDSA through higher eukaryotes has been a matter of debate. Here, we demonstrate the function of Blm in SDSA type HR in chicken DT40 B lymphocyte line, where Ig gene conversion diversifies the immunoglobulin V gene through intragenic HR between diverged homologous segments. This reaction is initiated by the activation-induced cytidine deaminase enzyme-mediated uracil formation at the V gene, which in turn converts into abasic site, presumably leading to a single strand gap. Ig gene conversion frequency was drastically reduced in BLM−/− cells. In addition, BLM−/− cells used limited donor segments harboring higher identity compared with other segments in Ig gene conversion event, suggesting that Blm can promote HR between diverged sequences. To further understand the role of Blm in HR between diverged homologous sequences, we measured the frequency of gene targeting induced by an I-SceI-endonuclease-mediated double-strand break. BLM−/− cells showed a severer defect in the gene targeting frequency as the number of heterologous sequences increased at the double-strand break site. Conversely, the overexpression of Blm, even an ATPase-defective mutant, strongly stimulated gene targeting. In summary, Blm promotes HR between diverged sequences through a novel ATPase-independent mechanism. PMID:19661064

Navigable Waters Web Map Disclaimer

Science.gov Websites

/ Department of Law Reports & Letters Recordable Disclaimers of Interest Fact Sheet (PDF) State's Recordable Disclaimers of Interest for Navigable Waters BLM's website for Recordable Disclaimers of Interest

75 FR 42040 - Endangered and Threatened Wildlife and Plants; 12-Month Finding on a Petition to List the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-20

... Group The Harlan-Keal Group consists of four sites: 5 ac (2 ha) of private land (Harlan-Keal), including... seeps occur on lands administered by the BLM. The Harlan-Keal pond was restored in 2003-2004, and has... surveys. The Crystal Spring site consists of a spring, pond, and outflow on BLM land. In 1995, a wild...

43 CFR 2806.30 - What are the rents for communication site rights-of-way?

Code of Federal Regulations, 2012 CFR

2012-10-01

... communication use rent schedule on the BLM Home Page on the Internet at http://www.blm.gov. (2) BLM will revise...: Communication Use Rent Schedule Annual Fees [Calendar year 2005] Population Television broadcast Am/FM radio...,000 1,489.63 1,117.22 744.81 124.14 744.81 3,103.39 434.47 1,862.03 93.10 1 Rent for AM Radio is 70...

43 CFR 2806.30 - What are the rents for communication site rights-of-way?

Code of Federal Regulations, 2013 CFR

2013-10-01

... communication use rent schedule on the BLM Home Page on the Internet at http://www.blm.gov. (2) BLM will revise...: Communication Use Rent Schedule Annual Fees [Calendar year 2005] Population Television broadcast Am/FM radio...,000 1,489.63 1,117.22 744.81 124.14 744.81 3,103.39 434.47 1,862.03 93.10 1 Rent for AM Radio is 70...

43 CFR 2806.30 - What are the rents for communication site rights-of-way?

Code of Federal Regulations, 2014 CFR

2014-10-01

... communication use rent schedule on the BLM Home Page on the Internet at http://www.blm.gov. (2) BLM will revise...: Communication Use Rent Schedule Annual Fees [Calendar year 2005] Population Television broadcast Am/FM radio...,000 1,489.63 1,117.22 744.81 124.14 744.81 3,103.39 434.47 1,862.03 93.10 1 Rent for AM Radio is 70...

RECQ-like helicases Sgs1 and BLM regulate R-loop–associated genome instability

PubMed Central

Chang, Emily Yun-Chia; Novoa, Carolina A.; Aristizabal, Maria J.; Coulombe, Yan; Segovia, Romulo; Shen, Yaoqing; Keong, Christelle; Tam, Annie S.; Jones, Steven J.M.; Masson, Jean-Yves; Kobor, Michael S.

2017-01-01

Sgs1, the orthologue of human Bloom’s syndrome helicase BLM, is a yeast DNA helicase functioning in DNA replication and repair. We show that SGS1 loss increases R-loop accumulation and sensitizes cells to transcription–replication collisions. Yeast lacking SGS1 accumulate R-loops and γ-H2A at sites of Sgs1 binding, replication pausing regions, and long genes. The mutation signature of sgs1Δ reveals copy number changes flanked by repetitive regions with high R-loop–forming potential. Analysis of BLM in Bloom’s syndrome fibroblasts or by depletion of BLM from human cancer cells confirms a role for Sgs1/BLM in suppressing R-loop–associated genome instability across species. In support of a potential direct effect, BLM is found physically proximal to DNA:RNA hybrids in human cells, and can efficiently unwind R-loops in vitro. Together, our data describe a conserved role for Sgs1/BLM in R-loop suppression and support an increasingly broad view of DNA repair and replication fork stabilizing proteins as modulators of R-loop–mediated genome instability. PMID:29042409

RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.

PubMed

Chang, Emily Yun-Chia; Novoa, Carolina A; Aristizabal, Maria J; Coulombe, Yan; Segovia, Romulo; Chaturvedi, Richa; Shen, Yaoqing; Keong, Christelle; Tam, Annie S; Jones, Steven J M; Masson, Jean-Yves; Kobor, Michael S; Stirling, Peter C

2017-12-04

Sgs1, the orthologue of human Bloom's syndrome helicase BLM, is a yeast DNA helicase functioning in DNA replication and repair. We show that SGS1 loss increases R-loop accumulation and sensitizes cells to transcription-replication collisions. Yeast lacking SGS1 accumulate R-loops and γ-H2A at sites of Sgs1 binding, replication pausing regions, and long genes. The mutation signature of sgs1 Δ reveals copy number changes flanked by repetitive regions with high R-loop-forming potential. Analysis of BLM in Bloom's syndrome fibroblasts or by depletion of BLM from human cancer cells confirms a role for Sgs1/BLM in suppressing R-loop-associated genome instability across species. In support of a potential direct effect, BLM is found physically proximal to DNA:RNA hybrids in human cells, and can efficiently unwind R-loops in vitro. Together, our data describe a conserved role for Sgs1/BLM in R-loop suppression and support an increasingly broad view of DNA repair and replication fork stabilizing proteins as modulators of R-loop-mediated genome instability. © 2017 Chang et al.

Digital Database of Selected Aggregate and Related Resources in Ada, Boise, Canyon, Elmore, Gem, and Owyhee Counties, Southwestern Idaho

USGS Publications Warehouse

Moyle, Phillip R.; Wallis, John C.; Bliss, James D.; Bolm, Karen D.

2004-01-01

The U.S. Geological Survey (USGS) compiled a database of aggregate sites and geotechnical sample data for six counties - Ada, Boise, Canyon, Elmore, Gem, and Owyhee - in southwest Idaho as part of a series of studies in support of the Bureau of Land Management (BLM) planning process. Emphasis is placed on sand and gravel sites in deposits of the Boise River, Snake River, and other fluvial systems and in Neogene lacustrine deposits. Data were collected primarily from unpublished Idaho Transportation Department (ITD) records and BLM site descriptions, published Army Corps of Engineers (ACE) records, and USGS sampling data. The results of this study provides important information needed by land-use planners and resource managers, particularly in the BLM, to anticipate and plan for demand and development of sand and gravel and other mineral material resources on public lands in response to the urban growth in southwestern Idaho.

77 FR 46112 - Call for Nominations for Advisory Groups, Oregon/Washington

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-02

... Steens Mountain Advisory Council. Citizens who serve on these groups provide advice and recommendations to the BLM on land use planning and management of the National System of Public Lands within their geographic areas and management options for a specific National Landscape Conservation System site. The BLM...

BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription

PubMed Central

Grierson, Patrick M.; Lillard, Kate; Behbehani, Gregory K.; Combs, Kelly A.; Bhattacharyya, Saumitri; Acharya, Samir; Groden, Joanna

2012-01-01

Bloom's syndrome (BS) is an autosomal recessive disorder that is invariably characterized by severe growth retardation and cancer predisposition. The Bloom's syndrome helicase (BLM), mutations of which lead to BS, localizes to promyelocytic leukemia protein bodies and to the nucleolus of the cell, the site of RNA polymerase I-mediated ribosomal RNA (rRNA) transcription. rRNA transcription is fundamental for ribosome biogenesis and therefore protein synthesis, cellular growth and proliferation; its inhibition limits cellular growth and proliferation as well as bodily growth. We report that nucleolar BLM facilitates RNA polymerase I-mediated rRNA transcription. Immunofluorescence studies demonstrate the dependance of BLM nucleolar localization upon ongoing RNA polymerase I-mediated rRNA transcription. In vivo protein co-immunoprecipitation demonstrates that BLM interacts with RPA194, a subunit of RNA polymerase I. 3H-uridine pulse-chase assays demonstrate that BLM expression is required for efficient rRNA transcription. In vitro helicase assays demonstrate that BLM unwinds GC-rich rDNA-like substrates that form in the nucleolus and normally inhibit progression of the RNA polymerase I transcription complex. These studies suggest that nucleolar BLM modulates rDNA structures in association with RNA polymerase I to facilitate RNA polymerase I-mediated rRNA transcription. Given the intricate relationship between rDNA metabolism and growth, our data may help in understanding the etiology of proportional dwarfism in BS. PMID:22106380

BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription.

PubMed

Grierson, Patrick M; Lillard, Kate; Behbehani, Gregory K; Combs, Kelly A; Bhattacharyya, Saumitri; Acharya, Samir; Groden, Joanna

2012-03-01

Bloom's syndrome (BS) is an autosomal recessive disorder that is invariably characterized by severe growth retardation and cancer predisposition. The Bloom's syndrome helicase (BLM), mutations of which lead to BS, localizes to promyelocytic leukemia protein bodies and to the nucleolus of the cell, the site of RNA polymerase I-mediated ribosomal RNA (rRNA) transcription. rRNA transcription is fundamental for ribosome biogenesis and therefore protein synthesis, cellular growth and proliferation; its inhibition limits cellular growth and proliferation as well as bodily growth. We report that nucleolar BLM facilitates RNA polymerase I-mediated rRNA transcription. Immunofluorescence studies demonstrate the dependance of BLM nucleolar localization upon ongoing RNA polymerase I-mediated rRNA transcription. In vivo protein co-immunoprecipitation demonstrates that BLM interacts with RPA194, a subunit of RNA polymerase I. (3)H-uridine pulse-chase assays demonstrate that BLM expression is required for efficient rRNA transcription. In vitro helicase assays demonstrate that BLM unwinds GC-rich rDNA-like substrates that form in the nucleolus and normally inhibit progression of the RNA polymerase I transcription complex. These studies suggest that nucleolar BLM modulates rDNA structures in association with RNA polymerase I to facilitate RNA polymerase I-mediated rRNA transcription. Given the intricate relationship between rDNA metabolism and growth, our data may help in understanding the etiology of proportional dwarfism in BS.

Predicting toxic effects of copper on aquatic biota in mineralized areas by using the Biotic Ligand Model

USGS Publications Warehouse

Smith, Kathleen S.; Ranville, James F.; Adams, M.; Choate, LaDonna M.; Church, Stan E.; Fey, David L.; Wanty, Richard B.; Crock, James G.

2006-01-01

The chemical speciation of metals influences their biological effects. The Biotic Ligand Model (BLM) is a computational approach to predict chemical speciation and acute toxicological effects of metals on aquatic biota. Recently, the U.S. Environmental Protection Agency incorporated the BLM into their regulatory water-quality criteria for copper. Results from three different laboratory copper toxicity tests were compared with BLM predictions for simulated test-waters. This was done to evaluate the ability of the BLM to accurately predict the effects of hardness and concentrations of dissolved organic carbon (DOC) and iron on aquatic toxicity. In addition, we evaluated whether the BLM and the three toxicity tests provide consistent results. Comparison of BLM predictions with two types of Ceriodaphnia dubia toxicity tests shows that there is fairly good agreement between predicted LC50 values computed by the BLM and LC50 values determined from the two toxicity tests. Specifically, the effect of increasing calcium concentration (and hardness) on copper toxicity appears to be minimal. Also, there is fairly good agreement between the BLM and the two toxicity tests for test solutions containing elevated DOC, for which the LC50 is 3-to-5 times greater (less toxic) than the LC50 for the lower-DOC test water. This illustrates the protective effects of DOC on copper toxicity and demonstrates the ability of the BLM to predict these protective effects. In contrast, for test solutions with added iron there is a decrease in LC50 values (increase in toxicity) in results from the two C. dubia toxicity tests, and the agreement between BLM LC50 predictions and results from these toxicity tests is poor. The inability of the BLM to account for competitive iron binding to DOC or DOC fractionation may be a significant shortcoming of the BLM for predicting site- specific water-quality criteria in streams affected by iron-rich acidic drainage in mined and mineralized areas.

Application of Biotic Ligand and Toxic Unit modeling approaches to predict improvements in zooplankton species richness in smelter-damaged lakes near Sudbury, Ontario.

PubMed

Khan, Farhan R; Keller, W Bill; Yan, Norman D; Welsh, Paul G; Wood, Chris M; McGeer, James C

2012-02-07

Using a 30-year record of biological and water chemistry data collected from seven lakes near smelters in Sudbury (Ontario, Canada) we examined the link between reductions of Cu, Ni, and Zn concentrations and zooplankton species richness. The toxicity of the metal mixtures was assessed using an additive Toxic Unit (TU) approach. Four TU models were developed based on total metal concentrations (TM-TU); free ion concentrations (FI-TU); acute LC50s calculated from the Biotic Ligand Model (BLM-TU); and chronic LC50s (acute LC50s adjusted by metal-specific acute-to-chronic ratios, cBLM-TU). All models significantly correlated reductions in metal concentrations to increased zooplankton species richness over time (p < 0.01) with a rank based on r(2) values of cBLM-TU > BLM-TU = FI-TU > TM-TU. Lake-wise comparisons within each model showed that the BLM-TU and cBLM-TU models provided the best description of recovery across all seven lakes. These two models were used to calculate thresholds for chemical and biological recovery using data from reference lakes in the same region. A threshold value of TU = 1 derived from the cBLM-TU provided the most accurate description of recovery. Overall, BLM-based TU models that integrate site-specific water chemistry-derived estimates of toxicity offer a useful predictor of biological recovery.

43 CFR 3602.42 - How does BLM publicize competitive mineral materials sales?

Code of Federal Regulations, 2011 CFR

2011-10-01

... where the material is located, on the same day once a week for 2 consecutive weeks; and (2) Will post a... are offering; (4) The unit of measurement; (5) The appraised prices; (6) The time and place for... cost recovery fee paid to BLM by the applicant up to 21 days before the sale; (9) The site access that...

Accurate and rapid modeling of iron-bleomycin-induced DNA damage using tethered duplex oligonucleotides and electrospray ionization ion trap mass spectrometric analysis.

PubMed

Harsch, A; Marzilli, L A; Bunt, R C; Stubbe, J; Vouros, P

2000-05-01

Bleomycin B(2)(BLM) in the presence of iron [Fe(II)] and O(2)catalyzes single-stranded (ss) and double-stranded (ds) cleavage of DNA. Electrospray ionization ion trap mass spectrometry was used to monitor these cleavage processes. Two duplex oligonucleotides containing an ethylene oxide tether between both strands were used in this investigation, allowing facile monitoring of all ss and ds cleavage events. A sequence for site-specific binding and cleavage by Fe-BLM was incorporated into each analyte. One of these core sequences, GTAC, is a known hot-spot for ds cleavage, while the other sequence, GGCC, is a hot-spot for ss cleavage. Incubation of each oligo-nucleotide under anaerobic conditions with Fe(II)-BLM allowed detection of the non-covalent ternary Fe-BLM/oligonucleotide complex in the gas phase. Cleavage studies were then performed utilizing O(2)-activated Fe(II)-BLM. No work-up or separation steps were required and direct MS and MS/MS analyses of the crude reaction mixtures confirmed sequence-specific Fe-BLM-induced cleavage. Comparison of the cleavage patterns for both oligonucleotides revealed sequence-dependent preferences for ss and ds cleavages in accordance with previously established gel electrophoresis analysis of hairpin oligonucleotides. This novel methodology allowed direct, rapid and accurate determination of cleavage profiles of model duplex oligonucleotides after exposure to activated Fe-BLM.

78 FR 39311 - Notice of Intent To Prepare a Resource Management Plan Amendment and an Associated Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-01

... approximately 45 acres at the top of Glass Buttes from a VRM class 2 to a VRM class 4. The BLM intends to... atop Glass Buttes in the BLM Prineville District. This notice announces the beginning of the scoping... may submit comments on issues and planning criteria related to the Glass Buttes Communication Site and...

75 FR 65650 - Notice of Availability of the Record of Decision for the Calico Solar Project and Associated...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-26

..., the BLM is also amending the CDCA Plan to allow for the siting of a solar energy power plant on the... Calico Solar Project into the Energy Production and Utility Corridors Element of the CDCA Plan. The BLM's..., LVRAM109AA03] Notice of Availability of the Record of Decision for the Calico Solar Project and Associated...

Structural basis of Bloom syndrome (BS) causing mutations in the BLM helicase domain.

PubMed Central

Rong, S. B.; Väliaho, J.; Vihinen, M.

2000-01-01

BACKGROUND: Bloom syndrome (BS) is characterized by mutations within the BLM gene. The Bloom syndrome protein (BLM) has similarity to the RecQ subfamily of DNA helicases, which contain seven conserved helicase domains and share significant sequence and structural similarity with the Rep and PcrA DNA helicases. We modeled the three-dimensional structure of the BLM helicase domain to analyze the structural basis of BS-causing mutations. MATERIALS AND METHODS: The sequence alignment was performed for RecQ DNA helicases and Rep and PcrA helicases. The crystal structure of PcrA helicase (PDB entry 3PJR) was used as the template for modeling the BLM helicase domain. The model was used to infer the function of BLM and to analyze the effect of the mutations. RESULTS: The structural model with good stereochemistry of the BLM helicase domain contains two subdomains, 1A and 2A. The electrostatic potential of the model is highly negative over most of the surface, except for the cleft between subdomains 1A and 2A which is similar to the template protein. The ATP-binding site is located inside the model between subdomains 1A and 2A; whereas, the DNA-binding region is situated at the surface cleft, with positive potential between 1A and 2A. CONCLUSIONS: The three-dimensional structure of the BLM helicase domain was modeled and applied to interpret BS-causing mutations. The mutation I841T is likely to weaken DNA binding, while the mutations C891R, C901Y, and Q672R presumably disturb the ATP binding. In addition, other critical positions are discussed. PMID:10965492

Mitigation and Monitoring Plan for impacted wetlands at the Gunnison UMTRA Project site, Gunnison, Colorado. Revision 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1994-12-01

The Gunnison, Colorado, abandoned uranium mill site is one site being cleaned up by the DOE under UMTRCA authority. This site`s contaminated material is being transported to a disposal site on US Bureau of Land Management (BLM) land east of Gunnison. Remedial action activities have temporarily disturbed 0.8 acre (ac) (0.3 hectares [ha]) of wetlands and permanently eliminated 4.3 ac (1.7 ha). As required by the Clean Water Act, the US Army Corps of Engineers (USACE) prepared a Section 404 Permit that addresses the loss of wetlands as a result of remedial action at the Gunnison UMTRA Project site. Themore » 404 permit includes this report as an attachment and it describes the wetland mitigation and monitoring plan. The DOE formulated this plan in consultation with the BLM and the USACE. This report represents a revised version of the mitigation and monitoring plan (DOE, 1992b).« less

Geochemical investigations and interim recommendations for priority abandoned mine sites, BLM lands, upper Animas River watershed, San Juan County, Colorado

USGS Publications Warehouse

Nash, J. Thomas

1999-01-01

Field observations, sampling of mine dumps and mine drainage waters, and laboratory studies of dump materials have been made at mining areas deemed to be on public lands administered by the U. S. Bureau of Land Management (BLM) in the Upper Animas River watershed. Results of chemical analyses of dump materials, leachates of those materials, and surface waters draining mines or dumps provide indications of where acid is generated or consumed, and metal concentrations below mines or dumps. Information on sites previously identified as needing reclamation is reviewed and available geochemical information is used to rank 26 sites into four classes of priority for reclamation. Although there are more than a thousand mining sites (productive mines and prospects) on BLM lands in the Upper Animas River watershed study area, the majority are very small (less than about 70 cubic yards of dump material), are more than 2 miles from a major stream, or so inaccessible as to prohibit reclamation. In the summers of 1997 and 1998 approximately 200 sites were observed and more than 100 of these that appeared to have the potential to geochemically impact the watershed were examined more carefully and sampled. Building upon the prior work of the BLM and associated agencies, this work attempted to identify the most significant sources of mine-related contamination and to rank those sites as to priority for reclamation. These most significant mining areas have been examined within a geologic framework and were evaluated by multiple criteria, including tendency to generate acid and release toxic metals, observed damage to vegetation, potential to release metals based on leach tests, and likelihood of transport into streams of the watershed. No single measurable parameter, such as metal concentration, can be used to rank the sites. Rather, subjective estimates are required to evaluate combinations or interactions among several parameters. The most subjective estimate, while ranking feasibility of reclamation, is the relative amounts of naturally occurring metals and acidity and mine-related contamination at each mining area. Mitigation of natural contributions at mines or unmined areas is beyond the scope of my studies, but should be considered when planning reclamation. Available information for the 26 priority sites is adequate for ranking, but at some sites additional information on groundwater conditions is needed for a more complete site evaluation.

Regulation of gene expression by the BLM helicase correlates with the presence of G-quadruplex DNA motifs

PubMed Central

Nguyen, Giang Huong; Tang, Weiliang; Robles, Ana I.; Beyer, Richard P.; Gray, Lucas T.; Welsh, Judith A.; Schetter, Aaron J.; Kumamoto, Kensuke; Wang, Xin Wei; Hickson, Ian D.; Maizels, Nancy; Monnat, Raymond J.; Harris, Curtis C.

2014-01-01

Bloom syndrome is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition, and caused by mutations in the gene encoding the Bloom syndrome, RecQ helicase-like (BLM) protein. To determine whether altered gene expression might be responsible for pathological features of Bloom syndrome, we analyzed mRNA and microRNA (miRNA) expression in fibroblasts from individuals with Bloom syndrome and in BLM-depleted control fibroblasts. We identified mRNA and miRNA expression differences in Bloom syndrome patient and BLM-depleted cells. Differentially expressed mRNAs are connected with cell proliferation, survival, and molecular mechanisms of cancer, and differentially expressed miRNAs target genes involved in cancer and in immune function. These and additional altered functions or pathways may contribute to the proportional dwarfism, elevated cancer risk, immune dysfunction, and other features observed in Bloom syndrome individuals. BLM binds to G-quadruplex (G4) DNA, and G4 motifs were enriched at transcription start sites (TSS) and especially within first introns (false discovery rate ≤ 0.001) of differentially expressed mRNAs in Bloom syndrome compared with normal cells, suggesting that G-quadruplex structures formed at these motifs are physiologic targets for BLM. These results identify a network of mRNAs and miRNAs that may drive the pathogenesis of Bloom syndrome. PMID:24958861

Regulation of gene expression by the BLM helicase correlates with the presence of G-quadruplex DNA motifs.

PubMed

Nguyen, Giang Huong; Tang, Weiliang; Robles, Ana I; Beyer, Richard P; Gray, Lucas T; Welsh, Judith A; Schetter, Aaron J; Kumamoto, Kensuke; Wang, Xin Wei; Hickson, Ian D; Maizels, Nancy; Monnat, Raymond J; Harris, Curtis C

2014-07-08

Bloom syndrome is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition, and caused by mutations in the gene encoding the Bloom syndrome, RecQ helicase-like (BLM) protein. To determine whether altered gene expression might be responsible for pathological features of Bloom syndrome, we analyzed mRNA and microRNA (miRNA) expression in fibroblasts from individuals with Bloom syndrome and in BLM-depleted control fibroblasts. We identified mRNA and miRNA expression differences in Bloom syndrome patient and BLM-depleted cells. Differentially expressed mRNAs are connected with cell proliferation, survival, and molecular mechanisms of cancer, and differentially expressed miRNAs target genes involved in cancer and in immune function. These and additional altered functions or pathways may contribute to the proportional dwarfism, elevated cancer risk, immune dysfunction, and other features observed in Bloom syndrome individuals. BLM binds to G-quadruplex (G4) DNA, and G4 motifs were enriched at transcription start sites (TSS) and especially within first introns (false discovery rate ≤ 0.001) of differentially expressed mRNAs in Bloom syndrome compared with normal cells, suggesting that G-quadruplex structures formed at these motifs are physiologic targets for BLM. These results identify a network of mRNAs and miRNAs that may drive the pathogenesis of Bloom syndrome.

Framework for Derivation of Water Quality Criteria Using the Biotic Ligand Model: Copper as a Case Study.

PubMed

Gondek, John C; Gensemer, Robert W; Claytor, Carrie A; Canton, Steven P; Gorsuch, Joseph W

2018-06-01

Acceptance of the Biotic Ligand Model (BLM) to derive aquatic life criteria, for metals in general and copper in particular, is growing amongst regulatory agencies worldwide. Thus, it is important to ensure that water quality data are used appropriately and consistently in deriving such criteria. Here we present a suggested BLM implementation framework (hereafter referred to as "the Framework") to help guide the decision-making process when designing sampling and analysis programs for use of the BLM to derive water quality criteria applied on a site-specific basis. Such a framework will help inform stakeholders on the requirements needed to derive BLM-based criteria, and thus, ensure the appropriate types and amount of data are being collected and interpreted. The Framework was developed for calculating BLM-based criteria when data are available from multiple sampling locations on a stream. The Framework aspires to promote consistency when applying the BLM across datasets of disparate water quality, data quantity, and spatial and temporal representativeness, and is meant to be flexible to maximize applicability over a wide range of scenarios. Therefore, the Framework allows for a certain level of interpretation and adjustment to address the issues unique to each dataset. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Protectiveness of water quality criteria for copper in western United States waters relative to predicted olfactory responses in juvenile Pacific salmon.

PubMed

DeForest, David K; Gensemer, Robert W; Van Genderen, Eric J; Gorsuch, Joseph W

2011-07-01

Copper (Cu) can impair olfaction in juvenile Pacific salmon (as well as other fishes), thus potentially inhibiting the ability of juveniles to avoid predators or to find food. Because Cu is commonly elevated in stormwater runoff in urban environments, storm events may result in elevated Cu concentrations in salmon-bearing streams. Accordingly, there is concern that existing Cu criteria, which were not derived using data for olfactory-related endpoints, may not be adequately protective of juvenile salmon. However, a modification of the US Environmental Protection Agency (USEPA) biotic ligand model (BLM) for deriving site-specific Cu criteria was recently proposed, which accounted for the sensitivity of olfactory endpoints. The modification was based on olfactory inhibition in juvenile coho salmon (Oncorhynchus kisutch) exposed to Cu in various combinations of pH, hardness, alkalinity, and dissolved organic carbon (DOC) concentrations. We used that olfactory-based BLM to derive 20% inhibition concentrations (IC20) values for Cu for 133 stream locations in the western United States. The olfactory BLM-based IC20 values were compared to the existing hardness-based Cu criteria and the USEPA's BLM-based Cu criteria for these representative natural waters of the western United States. Of the 133 sampling locations, mean hardness-dependent acute and chronic Cu criteria were below the mean olfactory-based BLM IC20 value in 122 (92%) and 129 (97%) of the waters, respectively (i.e., <20% olfactory impairment would have been predicted at the mean hardness-based Cu criteria concentrations). Waters characterized by a combination of high hardness and very low DOC were most likely to have hardness-based Cu criteria that were higher than the olfactory-based BLM IC20 values, because DOC strongly influences Cu bioavailability in the BLM. In all waters, the USEPA's current BLM-based criteria were below the mean olfactory-based BLM IC20 values, indicating that the USEPA's BLM-based criteria are protective of olfactory impairment in juvenile salmon. Copyright © 2011 SETAC.

77 FR 44155 - Administration of Mining Claims and Sites

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-27

... 1004-AE27 Administration of Mining Claims and Sites AGENCY: Bureau of Land Management, Interior. ACTION... on locating, recording, and maintaining mining claims or sites. In this rule, the BLM amends its... placer mining claims. The law specifies that the holder of an unpatented placer mining claim must pay the...

Abandoned Uranium Mines (AUM) Site Screening Map Service, 2016, US EPA Region 9

EPA Pesticide Factsheets

As described in detail in the Five-Year Report, US EPA completed on-the-ground screening of 521 abandoned uranium mine areas. US EPA and the Navajo EPA are using the Comprehensive Database and Atlas to determine which mines should be cleaned up first. US EPA continues to research and identify Potentially Responsible Parties (PRPs) under Superfund to contribute to the costs of cleanup efforts.This US EPA Region 9 web service contains the following map layers:Abandoned Uranium Mines, Priority Mines, Tronox Mines, Navajo Environmental Response Trust Mines, Mines with Enforcement Actions, Superfund AUM Regions, Navajo Nation Administrative Boundaries and Chapter Houses.Mine points have a maximum scale of 1:220,000, while Mine polygons have a minimum scale of 1:220,000. Chapter houses have a minimum scale of 1:200,000. BLM Land Status has a minimum scale of 1:150,000.Full FGDC metadata records for each layer can be found by clicking the layer name at the web service endpoint and viewing the layer description. Data used to create this web service are available for download at https://edg.epa.gov/metadata/catalog/data/data.page.Security Classification: Public. Access Constraints: None. Use Constraints: None. Please check sources, scale, accuracy, currentness and other available information. Please confirm that you are using the most recent copy of both data and metadata. Acknowledgement of the EPA would be appreciated.

Review of samples of tailings, soils and stream sediment adjacent to and downstream from the Ruth Mine, Inyo County, California

USGS Publications Warehouse

Rytuba, James J.; Kim, Christopher S.; Goldstein, Daniel N.

2011-01-01

The Ruth Mine and mill are located in the western Mojave Desert in Inyo County, California (fig. 1). The mill processed gold-silver (Au-Ag) ores mined from the Ruth Au-Ag deposit, which is adjacent to the mill site. The Ruth Au-Ag deposit is hosted in Mesozoic intrusive rocks and is similar to other Au-Ag deposits in the western Mojave Desert that are associated with Miocene volcanic centers that formed on a basement of Mesozoic granitic rocks (Bateman, 1907; Gardner, 1954; Rytuba, 1996). The volcanic rocks consist of silicic domes and associated flows, pyroclastic rocks, and subvolcanic intrusions (fig. 2) that were emplaced into Mesozoic silicic intrusive rocks (Troxel and Morton, 1962). The Ruth Mine is on Federal land managed by the U.S. Bureau of Land Management (BLM). Tailings from the mine have been eroded and transported downstream into Homewood Canyon and then into Searles Valley (figs. 3, 4, 5, and 6). The BLM provided recreational facilities at the mine site for day-use hikers and restored and maintained the original mine buildings in collaboration with local citizen groups for use by visitors (fig. 7). The BLM requested that the U.S. Geological Survey (USGS), in collaboration with Chapman University, measure arsenic (As) and other geochemical constituents in soils and tailings at the mine site and in stream sediments downstream from the mine in Homewood Canyon and in Searles Valley (fig. 3). The request was made because initial sampling of the site by BLM staff indicated high concentrations of As in tailings and soils adjacent to the Ruth Mine. This report summarizes data obtained from field sampling of mine tailings and soils adjacent to the Ruth Mine and stream sediments downstream from the mine on June 7, 2009. Our results permit a preliminary assessment of the sources of As and associated chemical constituents that could potentially impact humans and biota.

43 CFR 3833.21 - When may I amend a notice or certificate of location?

Code of Federal Regulations, 2011 CFR

2011-10-01

... owners; (2) Relocate or re-establish mining claims or sites you previously forfeited or BLM declared void... mineral entry, unless— (1) You are reducing the size of the mining claim or site; (2) You need to correct...

43 CFR 3833.21 - When may I amend a notice or certificate of location?

Code of Federal Regulations, 2013 CFR

2013-10-01

... owners; (2) Relocate or re-establish mining claims or sites you previously forfeited or BLM declared void... mineral entry, unless— (1) You are reducing the size of the mining claim or site; (2) You need to correct...

43 CFR 3833.21 - When may I amend a notice or certificate of location?

Code of Federal Regulations, 2012 CFR

2012-10-01

... owners; (2) Relocate or re-establish mining claims or sites you previously forfeited or BLM declared void... mineral entry, unless— (1) You are reducing the size of the mining claim or site; (2) You need to correct...

43 CFR 3833.21 - When may I amend a notice or certificate of location?

Code of Federal Regulations, 2014 CFR

2014-10-01

... owners; (2) Relocate or re-establish mining claims or sites you previously forfeited or BLM declared void... mineral entry, unless— (1) You are reducing the size of the mining claim or site; (2) You need to correct...

Using biotic ligand models to predict metal toxicity in mineralized systems

USGS Publications Warehouse

Smith, Kathleen S.; Balistrieri, Laurie S.; Todd, Andrew S.

2015-01-01

The biotic ligand model (BLM) is a numerical approach that couples chemical speciation calculations with toxicological information to predict the toxicity of aquatic metals. This approach was proposed as an alternative to expensive toxicological testing, and the U.S. Environmental Protection Agency incorporated the BLM into the 2007 revised aquatic life ambient freshwater quality criteria for Cu. Research BLMs for Ag, Ni, Pb, and Zn are also available, and many other BLMs are under development. Current BLMs are limited to ‘one metal, one organism’ considerations. Although the BLM generally is an improvement over previous approaches to determining water quality criteria, there are several challenges in implementing the BLM, particularly at mined and mineralized sites. These challenges include: (1) historically incomplete datasets for BLM input parameters, especially dissolved organic carbon (DOC), (2) several concerns about DOC, such as DOC fractionation in Fe- and Al-rich systems and differences in DOC quality that result in variations in metal-binding affinities, (3) water-quality parameters and resulting metal-toxicity predictions that are temporally and spatially dependent, (4) additional influences on metal bioavailability, such as multiple metal toxicity, dietary metal toxicity, and competition among organisms or metals, (5) potential importance of metal interactions with solid or gas phases and/or kinetically controlled reactions, and (6) tolerance to metal toxicity observed for aquatic organisms living in areas with elevated metal concentrations.

Vegetation dynamics at a Mojave Desert restoration site, 1992 to 2007

Treesearch

Jeffrey E. Ott; E. Durant McArthur; Stewart C. Sanderson

2011-01-01

The Twist Hollow restoration site on BLM land near St. George, Utah, had been badly disturbed by sand mining, rock quarrying, dumping, off-road vehicles and target shooting prior to its closure and treatment. In December 1992 the site was sculpted and drill seeded with Indian ricegrass (Stipa hymenoides), sand dropseed (Sporobolus cryptandrus), galleta (Hilaria jamesii...

1997 Monitoring report for the Gunnison, Colorado Wetlands Mitigation Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1997-11-01

Under the Uranium Mill Tailings Remedial Action (UMTRA) Project, the U.S. Department of Energy (DOE) cleaned up uranium mill tailings and other surface contamination near the town of Gunnison, Colorado. Remedial action resulted in the elimination of 4.3 acres (ac) (1.7 hectares [ha]) of wetlands. This loss is mitigated by the enhancement of six spring-fed areas on Bureau of Land Management (BLM) land (mitigation sites). Approximately 254 ac (1 03.3 ha) were fenced at the six sites to exclude grazing livestock. Of the 254 ac (103.3 ha), 17.8 ac (7.2 ha) are riparian plant communities; the rest are sagebrush communities.more » Baseline grazed conditions of the riparian plant communities at the mitigation sites were measured prior to fencing. This report discusses results of the fourth year of a monitoring program implemented to document the response of vegetation and wildlife to the exclusion of livestock. Three criteria for determining success of the mitigation were established: plant height, vegetation density (bare ground), and vegetation diversity. By 1996, Prospector Spring, Upper Long`s Gulch, and Camp Kettle met the criteria. The DOE requested transfer of these sites to BLM for long-term oversight. The 1997 evaluation of the three remaining sites, discussed in this report, showed two sites (Houston Gulch and Lower Long`s Gulch) meet the criteria. The DOE will request the transfer of these two sites to the BLM for long-term oversight. The last remaining site, Sage Hen Spring, has met only two of the criteria (percent bare ground and plant height). The third criterion, vegetation diversity, was not met. The vegetation appears to be changing from predominantly wet species to drier upland species, although the reason for this change is uncertain. It may be due to below-normal precipitation in recent years, diversion of water from the spring to the stock tank, or manipulation of the hydrology farther up gradient.« less

DNA Helicase HIM-6/BLM Both Promotes MutSγ-Dependent Crossovers and Antagonizes MutSγ-Independent Interhomolog Associations During Caenorhabditis elegans Meiosis

PubMed Central

Schvarzstein, Mara; Pattabiraman, Divya; Libuda, Diana E.; Ramadugu, Ajit; Tam, Angela; Martinez-Perez, Enrique; Roelens, Baptiste; Zawadzki, Karl A.; Yokoo, Rayka; Rosu, Simona; Severson, Aaron F.; Meyer, Barbara J.; Nabeshima, Kentaro; Villeneuve, Anne M.

2014-01-01

Meiotic recombination is initiated by the programmed induction of double-strand DNA breaks (DSBs), lesions that pose a potential threat to the genome. A subset of the DSBs induced during meiotic prophase become designated to be repaired by a pathway that specifically yields interhomolog crossovers (COs), which mature into chiasmata that temporarily connect the homologs to ensure their proper segregation at meiosis I. The remaining DSBs must be repaired by other mechanisms to restore genomic integrity prior to the meiotic divisions. Here we show that HIM-6, the Caenorhabditis elegans ortholog of the RecQ family DNA helicase BLM, functions in both of these processes. We show that him-6 mutants are competent to load the MutSγ complex at multiple potential CO sites, to generate intermediates that fulfill the requirements of monitoring mechanisms that enable meiotic progression, and to accomplish and robustly regulate CO designation. However, recombination events at a subset of CO-designated sites fail to mature into COs and chiasmata, indicating a pro-CO role for HIM-6/BLM that manifests itself late in the CO pathway. Moreover, we find that in addition to promoting COs, HIM-6 plays a role in eliminating and/or preventing the formation of persistent MutSγ-independent associations between homologous chromosomes. We propose that HIM-6/BLM enforces biased outcomes of recombination events to ensure that both (a) CO-designated recombination intermediates are reliably resolved as COs and (b) other recombination intermediates reliably mature into noncrossovers in a timely manner. PMID:25053665

2004 annual progress report: Stratton Sagebrush Hydrology Study Area: Establishment of a long-term research site in a high-elevation sagebrush steppe

USGS Publications Warehouse

Schoenecker, Kate; Lange, Bob; Calton, Mike

2005-01-01

A third goal was to evaluate grazing management after a prescribed burn. The BLM widely uses prescribed burns as a tool for land management and grazing management. In general, BLM policy restricts grazing after a wildfire for two or more years. Some BLM offices allow no grazing after a wildfire or prescribed treatment for at least two years. Conversely, the RFO often allows grazing following a prescribed burn directly after the peak growing season the following year. This procedure is used for two years post-burn, after which grazing management is directed by local conditions and goals. We are investigating this practice to evaluate the effects on plant production and nutrient cycling. The RFO specifically wants to know if there are negative effects from grazing one season after a prescribed burn. 

An introduction to the special issue on ecological sites

USDA-ARS?s Scientific Manuscript database

The establishment of the Rangeland Interagency Ecological Site Manual by the Natural Resources Conservation Service (NRCS), Forest Service (FS), and the Bureau of Land Management (BLM) heralds a new era of rangeland management in the United States (http://www.fs.fed.us/biology/soil/Signed_RIESM_2010...

43 CFR 3273.12 - How will BLM review my site license application?

Code of Federal Regulations, 2011 CFR

2011-10-01

...) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE... information. (b) If your site license is located on geothermal leases where the surface is managed by the... requested by the Federal Energy Regulatory Commission. (d) If another Federal agency manages the surface, we...

14-3-3ε Boosts Bleomycin-induced DNA Damage Response by Inhibiting the Drug-Resistant Activity of MVP

PubMed Central

Tang, Siwei; Bai, Chen; Yang, Pengyuan; Chen, Xian

2013-01-01

Major vault protein (MVP) is the predominant constituent of the vault particle, the largest known ribonuclear protein complex. Although emerging evidences have been establishing the links between MVP (vault) and multidrug resistance (MDR), little is known regarding exactly how the MDR activity of MVP is modulated during cellular response to drug-induced DNA damage (DDR). Bleomycin (BLM), an anti-cancer drug, induces DNA double-stranded breaks (DSBs) and consequently triggers the cellular DDR. Due to its physiological implications in hepatocellular carcinoma (HCC) and cell fate decision, 14-3-3ε was chosen as the pathway-specific bait protein to identify the critical target(s) responsible for HCC MDR. By using LC-MS/MS-based proteomic approach, MVP was first identified in the BLM-induced 14-3-3ε interactome formed in HCC cells. Biological characterization revealed that MVP possesses specific activity to promote the resistance to the BLM-induced DDR. On the other hand, 14-3-3ε enhances BLM-induced DDR by interacting with MVP. Mechanistic investigation further revealed that 14-3-3ε, in a phosphorylation-dependent manner, binds to the phosphorylated sites at both Thr52 and Ser864 of the monomer of MVP. Consequently, the phosphorylation-dependent binding between 14-3-3ε and MVP inhibits the drug-resistant activity of MVP for an enhanced DDR to BLM treatment. Our findings provide an insight into the mechanism underlying how the BLM-induced interaction between 14-3-3ε and MVP modulates MDR, implicating novel strategy to overcome the chemotherapeutic resistance through interfering specific protein-protein interactions. PMID:23590642

Digital mining claim density map for federal lands in Nevada: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Nevada as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate Bureau of Land Management (BLM) State office. BLM maintains a cumulative computer listing of mining claims in the MCRS database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in Utah: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Utah as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate BLM State office. BLM maintains a cumulative computer listing of mining claims in the MCRS database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in Wyoming: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Wyoming as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate BLM State office. BLM maintains a cumulative computer listing of mining claims in the Mining Claim Recordation System (MCRS) database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in Colorado: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Colorado as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate BLM State office. BLM maintains a cumulative computer listing of mining claims in the Mining Claim Recordation System (MCRS) database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in California: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in California as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate BLM State office. BLM maintains a cumulative computer listing of mining claims in the MCRS database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in New Mexico: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in New Mexico as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate BLM State office. BLM maintains a cumulative computer listing of mining claims in the MCRS database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in Washington: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Washington as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate BLM State office. BLM maintains a cumulative computer listing of mining claims in the Mining Claim Recordation System (MCRS) database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in Arizona: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Arizona as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill, and tunnel sites must be recorded at the appropriate BLM State office. BLM maintains a cumulative computer listing of mining claims in the MCRS database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Transfer and Transition: Interagency Coordination for Managing Public Lands at UMTRCA Title II Sites in Wyoming – 16614

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shafer, David S.; Vanek, Tim; Ribeiro, Tracy

By the end of fiscal year 2025, the U.S. Department of Energy (DOE) Office of Legacy Management (LM) is anticipating adding 17 sites remediated under Title II of the Uranium Mill Tailings Radiation Control Act (UMTRCA) to the current inventory of 90 sites that it manages. Among the new sites are ones where federal public lands occur within the proposed long-term care boundary, the boundary determined by the Nuclear Regulatory Commission and LM as necessary to maintain site protectiveness for the entombed uranium mill tailings and residual groundwater contamination. For these sites, public land withdrawals for land and minerals willmore » need to be established. LM’s primary mission at UMTRCA sites is to protect the public and the environment from exposure to contamination at the sites. For the sites with public lands or federally controlled minerals that will be transferring to LM, the Office will apply to the Department of the Interior (DOI) Bureau of Land Management (BLM) for new, public land and mineral withdrawals. At most current LM UMTRCA sites that involved public lands and minerals, DOI granted DOE “full administrative jurisdiction” and permanent withdrawals. Hence, these withdrawals are, permanently, no longer subject to public land, mining, and mineral-leasing laws and regulations. LM is coordinating with DOI/BLM in Wyoming to permanently withdraw full and partial jurisdiction at future UMTRCA Title II sites in that state. This approach would allow LM to fully administer surface lands and minerals, where necessary, and DOI and LM to administer surface lands and leasable minerals where it would not jeopardize sites’ radiological safety and long-term public and environmental protection. This “shared-jurisdiction approach” will meet LM’s strategic goal of protecting human health and the environment but also allow BLM to fulfill their mission to “manage and conserve the lands under the mandate of multiple-use and sustained yield.” In addition, LM could also fulfill the fourth goal of its Strategic Plan, to optimize land use and assets.« less

Chk1 protects against chromatin bridges by constitutively phosphorylating BLM serine 502 to inhibit BLM degradation.

PubMed

Petsalaki, Eleni; Dandoulaki, Maria; Morrice, Nick; Zachos, George

2014-09-15

Chromatin bridges represent incompletely segregated chromosomal DNA connecting the anaphase poles and can result in chromosome breakage. The Bloom's syndrome protein helicase (BLM, also known as BLMH) suppresses formation of chromatin bridges. Here, we show that cells deficient in checkpoint kinase 1 (Chk1, also known as CHEK1) exhibit higher frequency of chromatin bridges and reduced BLM protein levels compared to controls. Chk1 inhibition leads to BLM ubiquitylation and proteasomal degradation during interphase. Furthermore, Chk1 constitutively phosphorylates human BLM at serine 502 (S502) and phosphorylated BLM localises to chromatin bridges. Mutation of S502 to a non-phosphorylatable alanine residue (BLM-S502A) reduces the stability of BLM, whereas expression of a phospho-mimicking BLM-S502D, in which S502 is mutated to aspartic acid, stabilises BLM and prevents chromatin bridges in Chk1-deficient cells. In addition, wild-type but not BLM-S502D associates with cullin 3, and cullin 3 depletion rescues BLM accumulation and localisation to chromatin bridges after Chk1 inhibition. We propose that Chk1 phosphorylates BLM-S502 to inhibit cullin-3-mediated BLM degradation during interphase. These results suggest that Chk1 prevents deleterious anaphase bridges by stabilising BLM. © 2014. Published by The Company of Biologists Ltd.

78 FR 68466 - BLM Director's Response to the Idaho Governor's Appeal of the BLM Idaho State Director's Governor...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-14

... Bureau of Land Management (BLM) is publishing this notice to explain why the BLM Director is denying the...] BLM Director's Response to the Idaho Governor's Appeal of the BLM Idaho State Director's Governor's... (Finding) to the BLM Idaho State Director (State Director). The State Director determined the Governor's...

DNA helicase HIM-6/BLM both promotes MutSγ-dependent crossovers and antagonizes MutSγ-independent interhomolog associations during caenorhabditis elegans meiosis.

PubMed

Schvarzstein, Mara; Pattabiraman, Divya; Libuda, Diana E; Ramadugu, Ajit; Tam, Angela; Martinez-Perez, Enrique; Roelens, Baptiste; Zawadzki, Karl A; Yokoo, Rayka; Rosu, Simona; Severson, Aaron F; Meyer, Barbara J; Nabeshima, Kentaro; Villeneuve, Anne M

2014-09-01

Meiotic recombination is initiated by the programmed induction of double-strand DNA breaks (DSBs), lesions that pose a potential threat to the genome. A subset of the DSBs induced during meiotic prophase become designated to be repaired by a pathway that specifically yields interhomolog crossovers (COs), which mature into chiasmata that temporarily connect the homologs to ensure their proper segregation at meiosis I. The remaining DSBs must be repaired by other mechanisms to restore genomic integrity prior to the meiotic divisions. Here we show that HIM-6, the Caenorhabditis elegans ortholog of the RecQ family DNA helicase BLM, functions in both of these processes. We show that him-6 mutants are competent to load the MutSγ complex at multiple potential CO sites, to generate intermediates that fulfill the requirements of monitoring mechanisms that enable meiotic progression, and to accomplish and robustly regulate CO designation. However, recombination events at a subset of CO-designated sites fail to mature into COs and chiasmata, indicating a pro-CO role for HIM-6/BLM that manifests itself late in the CO pathway. Moreover, we find that in addition to promoting COs, HIM-6 plays a role in eliminating and/or preventing the formation of persistent MutSγ-independent associations between homologous chromosomes. We propose that HIM-6/BLM enforces biased outcomes of recombination events to ensure that both (a) CO-designated recombination intermediates are reliably resolved as COs and (b) other recombination intermediates reliably mature into noncrossovers in a timely manner. Copyright © 2014 by the Genetics Society of America.

76 FR 45554 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-29

.... 20110231, Final EIS, BLM, NV, Salt Wells Energy Projects, Proposal for Three Separate Geothermal Energy and.... EIS No. 20110241, Draft EIS, NNSA, NV, Site-Wide EIS--Continued Operation of the Department of Energy...

Challenges for the development of a biotic ligand model predicting copper toxicity in estuaries and seas.

PubMed

de Polo, Anna; Scrimshaw, Mark D

2012-02-01

An effort is ongoing to develop a biotic ligand model (BLM) that predicts copper (Cu) toxicity in estuarine and marine environments. At present, the BLM accounts for the effects of water chemistry on Cu speciation, but it does not consider the influence of water chemistry on the physiology of the organisms. We discuss how chemistry affects Cu toxicity not only by controlling its speciation, but also by affecting the osmoregulatory physiology of the organism, which varies according to salinity. In an attempt to understand the mechanisms of Cu toxicity and predict its impacts, we explore the hypothesis that the common factor linking the main toxic effects of Cu is the enzyme carbonic anhydrase (CA), because it is a Cu target with multiple functions and salinity-dependent expression and activity. According to this hypothesis, the site of action of Cu in marine fish may be not only the gill, but also the intestine, because in this tissue CA plays an important role in ion transport and water adsorption. Therefore, the BLM of Cu toxicity to marine fish should also consider the intestine as a biotic ligand. Finally, we underline the need to incorporate the osmotic gradient into the BLM calculations to account for the influence of physiology on Cu toxicity. Copyright © 2011 SETAC.

Digital mining claim density map for federal lands in Idaho: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Idaho as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill and tunnel sites must be recorded at the appropriate Bureau of Land Management (BLM) State office. BLM maintains a cumulative computer listing of mining claims in the Mining Claim Recordation System (MCRS) database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Digital mining claim density map for federal lands in Oregon: 1996

USGS Publications Warehouse

Hyndman, Paul C.; Campbell, Harry W.

1999-01-01

This report describes a digital map generated by the U.S. Geological Survey (USGS) to provide digital spatial mining claim density information for federal lands in Oregon as of March 1997. Mining claim data is earth science information deemed to be relevant to the assessment of historic, current, and future ecological, economic, and social systems. There is no paper map included in this Open-File report. In accordance with the Federal Land Policy and Management Act of 1976 (FLPMA), all unpatented mining claims, mill and tunnel sites must be recorded at the appropriate Bureau of Land Management (BLM) State office. BLM maintains a cumulative computer listing of mining claims in the Mining Claim Recordation System (MCRS) database with locations given by meridian, township, range, and section. A mining claim is considered closed when the claim is relinquished or a formal BLM decision declaring the mining claim null and void has been issued and the appeal period has expired. All other mining claims filed with BLM are considered to be open and actively held. The digital map (figure 1.) with the mining claim density database available in this report are suitable for geographic information system (GIS)-based regional assessments at a scale of 1:100,000 or smaller.

Selective tumor cell targeting by the disaccharide moiety of bleomycin.

PubMed

Yu, Zhiqiang; Schmaltz, Ryan M; Bozeman, Trevor C; Paul, Rakesh; Rishel, Michael J; Tsosie, Krystal S; Hecht, Sidney M

2013-02-27

In a recent study, the well-documented tumor targeting properties of the antitumor agent bleomycin (BLM) were studied in cell culture using microbubbles that had been derivatized with multiple copies of BLM. It was shown that BLM selectively targeted MCF-7 human breast carcinoma cells but not the "normal" breast cell line MCF-10A. Furthermore, it was found that the BLM analogue deglycobleomycin, which lacks the disaccharide moiety of BLM, did not target either cell line, indicating that the BLM disaccharide moiety is necessary for tumor selectivity. Not resolved in the earlier study were the issues of whether the BLM disaccharide moiety alone is sufficient for tumor cell targeting and the possible cellular uptake of the disaccharide. In the present study, we conjugated BLM, deglycoBLM, and BLM disaccharide to the cyanine dye Cy5**. It was found that the BLM and BLM disaccharide conjugates, but not the deglycoBLM conjugate, bound selectively to MCF-7 cells and were internalized. The same was also true for the prostate cancer cell line DU-145 (but not for normal PZ-HPV-7 prostate cells) and for the pancreatic cancer cell line BxPC-3 (but not for normal SVR A221a pancreas cells). The targeting efficiency of the disaccharide was only slightly less than that of BLM in MCF-7 and DU-145 cells and comparable to that of BLM in BxPC-3 cells. These results establish that the BLM disaccharide is both necessary and sufficient for tumor cell targeting, a finding with obvious implications for the design of novel tumor imaging and therapeutic agents.

78 FR 4435 - BLM Director's Response to the Alaska Governor's Appeal of the BLM Alaska State Director's...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-22

... Bureau of Land Management (BLM) is publishing this notice to explain why the BLM Director is rejecting... Director's Response to the Alaska Governor's Appeal of the BLM Alaska State Director's Governor's... the BLM Alaska State Director. The State Director determined the Governor's Finding was outside the...

75 FR 32962 - Notice of Intent To Prepare a Resource Management Plan for the Carlsbad Field Office, New Mexico...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-10

... site. This document contains pertinent and descriptive information regarding planning issues... any participant who wishes to clarify the views he or she expressed. The BLM will evaluate identified...

43 CFR 3277.12 - What will BLM do if I do not comply with all BLM requirements pertaining to utilization operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... all BLM requirements pertaining to utilization operations? 3277.12 Section 3277.12 Public Lands... § 3277.12 What will BLM do if I do not comply with all BLM requirements pertaining to utilization... corrective action within a specific time period. If the noncompliance continues or is serious in nature, BLM...

The function of Xenopus Bloom's syndrome protein homolog (xBLM) in DNA replication

PubMed Central

Liao, Shuren; Graham, Jeanine; Yan, Hong

2000-01-01

The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised antibodies to it. Immunodepletion of xBLM from a Xenopus egg extract severely inhibits the replication of DNA in reconstituted nuclei. Moreover, the inhibition can be rescued by the addition of the recombinant xBLM protein. These results provide the first direct evidence that BLM plays an important role in DNA replication, suggesting that Bloom's syndrome may be the consequence of defective DNA replication. PMID:11040210

43 CFR 2568.91 - Is there land owned by the Federal government that BLM cannot convey to me even if I qualify?

Code of Federal Regulations, 2011 CFR

2011-10-01

... unit of government, or a person other than yourself; (g) Land withdrawn or reserved for national defense, other than the National Petroleum Reserve-Alaska; (h) National Forest land; or (i) Land selected...: (1) Land within a recorded mining claim; (2) Home sites; (3) Trade and manufacturing sites; (4...

43 CFR 2568.91 - Is there land owned by the Federal government that BLM cannot convey to me even if I qualify?

Code of Federal Regulations, 2012 CFR

2012-10-01

... unit of government, or a person other than yourself; (g) Land withdrawn or reserved for national defense, other than the National Petroleum Reserve-Alaska; (h) National Forest land; or (i) Land selected...: (1) Land within a recorded mining claim; (2) Home sites; (3) Trade and manufacturing sites; (4...

43 CFR 2568.91 - Is there land owned by the Federal government that BLM cannot convey to me even if I qualify?

Code of Federal Regulations, 2014 CFR

2014-10-01

... unit of government, or a person other than yourself; (g) Land withdrawn or reserved for national defense, other than the National Petroleum Reserve-Alaska; (h) National Forest land; or (i) Land selected...: (1) Land within a recorded mining claim; (2) Home sites; (3) Trade and manufacturing sites; (4...

43 CFR 2568.91 - Is there land owned by the Federal government that BLM cannot convey to me even if I qualify?

Code of Federal Regulations, 2013 CFR

2013-10-01

... unit of government, or a person other than yourself; (g) Land withdrawn or reserved for national defense, other than the National Petroleum Reserve-Alaska; (h) National Forest land; or (i) Land selected...: (1) Land within a recorded mining claim; (2) Home sites; (3) Trade and manufacturing sites; (4...

43 CFR 2806.43 - How does BLM calculate rent for passive reflectors and local exchange networks?

Code of Federal Regulations, 2013 CFR

2013-10-01

... MANAGEMENT (2000) RIGHTS-OF-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Rents Communication Site Rights... used to bend or ricochet electronic signals between active relay stations or between an active relay...

43 CFR 2806.43 - How does BLM calculate rent for passive reflectors and local exchange networks?

Code of Federal Regulations, 2012 CFR

2012-10-01

... MANAGEMENT (2000) RIGHTS-OF-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Rents Communication Site Rights... used to bend or ricochet electronic signals between active relay stations or between an active relay...

43 CFR 2806.43 - How does BLM calculate rent for passive reflectors and local exchange networks?

Code of Federal Regulations, 2014 CFR

2014-10-01

... MANAGEMENT (2000) RIGHTS-OF-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Rents Communication Site Rights... used to bend or ricochet electronic signals between active relay stations or between an active relay...

76 FR 38416 - Notice of Segregation of Public Lands in the States of Arizona, California, Colorado, Nevada, New...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-30

... protecting potential sites for future solar energy development. DATES: Effective Date: This segregation is... public lands that have been identified by the BLM as having the potential for solar energy generation...

78 FR 6832 - Call for Nominations for the John Day-Snake Resource Advisory Council, Oregon/Washington

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-31

... advice and recommendations to the BLM on land use planning and management of the National System of... System sites like the Spring Basin and Badlands Wilderness Areas as well as the Lower Deschutes and John...

The disaccharide moiety of bleomycin facilitates uptake by cancer cells.

PubMed

Schroeder, Benjamin R; Ghare, M Imran; Bhattacharya, Chandrabali; Paul, Rakesh; Yu, Zhiqiang; Zaleski, Paul A; Bozeman, Trevor C; Rishel, Michael J; Hecht, Sidney M

2014-10-01

The disaccharide moiety is responsible for the tumor cell targeting properties of bleomycin (BLM). While the aglycon (deglycobleomycin) mediates DNA cleavage in much the same fashion as bleomycin, it exhibits diminished cytotoxicity in comparison to BLM. These findings suggested that BLM might be modular in nature, composed of tumor-seeking and tumoricidal domains. To explore this possibility, BLM analogues were prepared in which the disaccharide moiety was attached to deglycobleomycin at novel positions, namely, via the threonine moiety or C-terminal substituent. The analogues were compared with BLM and deglycoBLM for DNA cleavage, cancer cell uptake, and cytotoxic activity. BLM is more potent than deglycoBLM in supercoiled plasmid DNA relaxation, while the analogue having the disaccharide on threonine was less active than deglycoBLM and the analogue containing the C-terminal disaccharide was slightly more potent. While having unexceptional DNA cleavage potencies, both glycosylated analogues were more cytotoxic to cultured DU145 prostate cancer cells than deglycoBLM. Dye-labeled conjugates of the cytotoxic BLM aglycons were used in imaging experiments to determine the extent of cell uptake. The rank order of internalization efficiencies was the same as their order of cytotoxicities toward DU145 cells. These findings establish a role for the BLM disaccharide in tumor targeting/uptake and suggest that the disaccharide moiety may be capable of delivering other cytotoxins to cancer cells. While the mechanism responsible for uptake of the BLM disaccharide selectively by tumor cells has not yet been established, data are presented which suggest that the metabolic shift to glycolysis in cancer cells may provide the vehicle for selective internalization.

Pore-Water Carbonate and Phosphate As Predictors of Arsenate Toxicity in Soil.

PubMed

Lamb, Dane T; Kader, Mohammed; Wang, Liang; Choppala, Girish; Rahman, Mohammad Mahmudur; Megharaj, Mallavarapu; Naidu, Ravi

2016-12-06

Phytotoxicity of inorganic contaminants is influenced by the presence of competing ions at the site of uptake. In this study, interaction of soil pore-water constituents with arsenate toxicity was investigated in cucumber (Cucumis sativa L) using 10 contrasting soils. Arsenate phytotoxicity was shown to be related to soluble carbonate and phosphate. The data indicated that dissolved phosphate and carbonate had an antagonistic impact on arsenate toxicity to cucumber. To predict arsenate phytotoxicity in soils with a diverse range of soil solution properties, both carbonate and phosphate were required. The relationship between arsenic and pore-water toxicity parameters was established initially using multiple regression. In addition, based on the relationship with carbonate and phosphate we successively applied a terrestrial biotic ligand-like model (BLM) including carbonate and phosphate. Estimated effective concentrations from the BLM-like parametrization were strongly correlated to measured arsenate values in pore-water (R 2 = 0.76, P < 0.001). The data indicates that an ion interaction model similar to the BLM for arsenate is possible, potentially improving current risk assessments at arsenic and co-contaminated soils.

Knockdown of RMI1 impairs DNA repair under DNA replication stress.

PubMed

Xu, Chang; Fang, Lianying; Kong, Yangyang; Xiao, Changyan; Yang, Mengmeng; Du, Li-Qing; Liu, Qiang

2017-12-09

RMI1 (RecQ-mediated genome instability protein 1) forms a conserved BTR complex with BLM, Topo IIIα, and RMI2, and its absence causes genome instability. It has been revealed that RMI1 localizes to nuclear foci with BLM and Topo IIIα in response to replication stress, and that RMI1 functions downstream of BLM in promoting replication elongation. However, the precise functions of RMI1 during replication stress are not completely understood. Here we report that RMI1 knockdown cells are hypersensitive to hydroxyurea (HU). Using comet assay, we show that RMI1 knockdown cells exhibit accumulation of broken DNAs after being released from HU treatment. Moreover, we demonstrate that RMI1 facilitates the recovery from activated checkpoint and resuming the cell cycle after replicative stress. Surprisingly, loss of RMI1 results in a failure of RAD51 loading onto DNA damage sites. These findings reveal the importance of RMI1 in response to replication stress, which could explain the molecular basis for its function in maintaining genome integrity. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Multiscale guidance and tools for implementing a landscape approach to resource management in the Bureau of Land Management

USGS Publications Warehouse

Carter, Sarah K.; Carr, Natasha B.; Miller, Kevin H.; Wood, David J.A.

2017-01-19

The Bureau of Land Management (BLM) is implementing a landscape approach to resource management (hereafter, landscape approach) to more effectively work with partners and understand the effects of management decisions. A landscape approach is a set of concepts and principles used to guide resource management when multiple stakeholders are involved and goals include diverse and sustainable social, environmental, and economic outcomes. Core principles of a landscape approach include seeking meaningful participation of diverse stakeholders, considering diverse resource values in multifunctional landscapes, acknowledging the tradeoffs needed to meet diverse objectives in the context of sustainable resource management, and addressing the complexity of social and ecological processes by embracing interdisciplinarity and considering multiple and broad spatial and temporal perspectives.In chapter 1, we outline the overall goal of this report: to provide a conceptual foundation and framework for implementing a landscape approach to resource management in the BLM, focusing on the role of multiscale natural resource monitoring and assessment information. In chapter 2, we describe a landscape approach to resource management. BLM actions taken to implement a landscape approach include a major effort to compile broad-scale data on natural resource status and condition across much of the west. These broadscale data now provide a regional context for interpreting monitoring data collected at individual sites and informing decisions made for local projects. We also illustrate the utility of using multiscale data to understand potential effects of different resource management decisions, define relevant terms in landscape ecology, and identify spatial scales at which planning and management decisions may be evaluated.In chapter 3, we describe how the BLM Rapid Ecoregional Assessment program and Assessment, Inventory and Monitoring program may be integrated to provide the multiscale monitoring data needed to inform a landscape approach. We propose six core, broad-scale indicators of natural resource status and condition: the amount, spatial distribution, patch size and connectivity of ecosystems and wildlife habitats, and the pattern of existing development across the landscape. Additional supplemental broad-scale indicators may include fire return intervals, distributions of invasive species, and vulnerability of ecosystems to a changing climate. Landscape intactness is an additional derived indicator that is calculated from one or more of the core and supplemental broad-scale indicators. We then outline a process for assessing broad-scale indicators that is consistent with the overall BLM Assessment, Inventory, and Monitoring process, facilitating development of a multiscale natural resource monitoring program. Finally, we describe how broad-scale indicators of natural resource status and condition may guide field monitoring implemented through the BLM Assessment, Inventory and Monitoring program and help address complex management questions.In chapter 4, we consider the specific question of assessing the ecological integrity of rangelands across the western United States. We first define ecological integrity and its relation to land health. We then suggest that a combination of six local-scale indicators collected through field sampling at individual sites and five complementary broad-scale indicators together provide information on the composition, structure, and function of rangelands. The terrestrial monitoring indicators collected at the level of individual field sites are the amount of bare ground, vegetation composition (including invasive plants and plants of management concern), vegetation height, and the proportion of the soil surface in large intercanopy gaps. The broad-scale indicators are vegetation amount, distribution, patch size, connectivity, and productivity, along with the pattern of terrestrial development. Our suggested approach to quantifying ecological integrity focuses specifically on informing management of public lands for multiple resource uses, and illustrates how existing data collected through BLM monitoring and assessment programs may be used together to provide multiscale information on land condition across broad extents.In chapter 5, we develop a method for quantifying landscape intactness and apply this method to the western United States. Our multiscale index of landscape intactness is designed to be defensible, decomposable, and easy to understand. The foundation of the multiscale index of landscape intactness is the surface disturbance footprint of anthropogenic development, including energy and urban development, roads and railroads, cultivated croplands, surface mines and quarries, and energy transmission lines and pipelines. The index represents a gradient of anthropogenic influence as represented by development summarized at two spatial scales of analysis: 2.5 and 20 kilometers. We provide several example applications of the index, illustrating how these data may inform natural resource decisions at the spatial extent of BLM field and district offices, states, ecoregions, and the western United States. We find that 19.2 percent of lands managed by the BLM across the 17 western states of the conterminous United States had the highest landscape intactness. The largest intact areas occur on public lands at high elevations or in the Great Basin.We believe the frameworks, processes, and analyses provided in this report will improve the ability of the BLM to identify and evaluate potential direct and indirect effects of management actions (such as habitat restoration and renewable energy development), and assist the BLM in further implementing a landscape approach to resource management.

78 FR 25097 - Notice of Realty Action: Competitive, Sealed-Bid, Spring SNPLMA Sale of Public Lands in Clark...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-29

... Determination of National Environmental Policy Act Adequacy (DNA). DATES: Interested parties may submit written... December 23, 2004, and a site specific DNA document numbered DOI-BLM-NV-S010-2012-0106-DNA. Sale procedures...

43 CFR 2806.30 - What are the rents for communication site rights-of-way?

Code of Federal Regulations, 2011 CFR

2011-10-01

.... You may obtain a copy of the current schedule from any BLM state or field office or by writing... mobile radio service Microwave Other communication uses 5,000,000 plus $55,861.13 $42,206.21 (2) (2) $14...

Airway transplantation of adipose stem cells protects against bleomycin-induced pulmonary fibrosis.

PubMed

Llontop, Pedro; Lopez-Fernandez, Daniel; Clavo, Bernardino; Afonso Martín, Juan Luis; Fiuza-Pérez, María D; García Arranz, Mariano; Calatayud, Joaquín; Molins López-Rodó, Laureano; Alshehri, Khalid; Ayub, Adil; Raad, Wissam; Bhora, Faiz; Santana-Rodríguez, Norberto

2018-04-01

Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with poor prognosis. Adipose-derived stem cells (ADSC) have demonstrated regenerative properties in several tissues. The hypothesis of this study was that airway transplantation of ADSC could protect against bleomycin (BLM)-induced pulmonary fibrosis (PF). Fifty-eight lungs from 29 male Sprague-Dawley rats were analyzed. Animals were randomly divided into five groups: a) control (n=3); b) sham (n=6); c) BLM (n=6); d) BLM+ADSC-2d (n=6); and e) BLM+ADSC-14d (n=8). Animals received 500 µL saline (sham), 2.5 UI/kg BLM in 500 µL saline (BLM), and 2×10 6  ADSC in 100 µL saline intratracheally at 2 (BLM+ADSC-2d) and 14 days (BLM+ADSC-14d) after BLM. Animals were sacrificed at 28 days. Blinded Ashcroft score was used to determine pulmonary fibrosis extent on histology. Hsp27, Vegf, Nfkβ, IL-1, IL-6, Col4, and Tgfβ1 mRNA gene expression were determined using real-time quantitative-PCR. Ashcroft index was: control=0; sham=0.37±0.07; BLM=6.55±0.34 vs sham (P=0.006). BLM vs BLM+ADSC-2d=4.63±0.38 (P=0.005) and BLM+ADSC-14d=3.77±0.46 (P=0.005). BLM vs sham significantly increased Hsp27 (P=0.018), Nfkβ (P=0.009), Col4 (P=0.004), Tgfβ1 (P=0.006) and decreased IL-1 (P=0.006). BLM+ADSC-2d vs BLM significantly decreased Hsp27 (P=0.009) and increased Vegf (P=0.006), Nfkβ (P=0.009). BLM+ADSC-14d vs BLM significantly decreased Hsp27 (P=0.028), IL-6 (P=0.013), Col4 (P=0.002), and increased Nfkβ (P=0.040) and Tgfβ1 (P=0.002). Airway transplantation of ADSC significantly decreased the fibrosis rate in both early and established pulmonary fibrosis, modulating the expression of Hsp27, Vegfa, Nfkβ, IL-6, Col4, and Tgfβ1. From a translational perspective, this technique could become a new adjuvant treatment for patients with IPF. © American Federation for Medical Research (unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Summary and findings of the 2006 BLM Forest Lands Report

Treesearch

Tim Bottomley; Jim Menlove

2009-01-01

In 2006, the Bureau of Land Management (BLM) contracted with the Forest Service Forest Inventory and Analysis Program (FIA) to assist in the preparation of a report specific to all forest lands under the administration of the BLM. The BLM requested that the FIA provide information on the extent and general conditions of BLM- managed forests and woodlands, within...

The DNA Helicase Activity of BLM Is Necessary for the Correction of the Genomic Instability of Bloom Syndrome Cells

PubMed Central

Neff, Norma F.; Ellis, Nathan A.; Ye, Tian Zhang; Noonan, James; Huang, Kelly; Sanz, Maureen; Proytcheva, Maria

1999-01-01

Bloom syndrome (BS) is a rare autosomal recessive disorder characterized by growth deficiency, immunodeficiency, genomic instability, and the early development of cancers of many types. BLM, the protein encoded by BLM, the gene mutated in BS, is localized in nuclear foci and absent from BS cells. BLM encodes a DNA helicase, and proteins from three missense alleles lack displacement activity. BLM transfected into BS cells reduces the frequency of sister chromatid exchanges and restores BLM in the nucleus. Missense alleles fail to reduce the sister chromatid exchanges in transfected BS cells or restore the normal nuclear pattern. BLM complements a phenotype of a Saccharomyces cerevisiae sgs1 top3 strain, and the missense alleles do not. This work demonstrates the importance of the enzymatic activity of BLM for its function and nuclear localization pattern. PMID:10069810

RECOVER - An Automated Burned Area Emergency Response Decision Support System for Post-fire Rehabilitation Management of Savanna Ecosystems in the Western US

NASA Astrophysics Data System (ADS)

Weber, K.; Schnase, J. L.; Carroll, M.; Brown, M. E.; Gill, R.; Haskett, G.; Gardner, T.

2013-12-01

In partnership with the Department of Interior's Bureau of Land Management (BLM) and the Idaho Department of Lands (IDL), we are building and evaluating the RECOVER decision support system. RECOVER - which stands for Rehabilitation Capability Convergence for Ecosystem Recovery - is an automatically deployable, context-aware decision support system for savanna wildfires that brings together in a single application the information necessary for post-fire rehabilitation decision-making and long-term ecosystem monitoring. RECOVER uses state-of-the-art cloud-based data management technologies to improve performance, reduce cost, and provide site-specific flexibility for each fire. The RECOVER Server uses Integrated Rule-Oriented Data System (iRODS) data grid technology deployed in the Amazon Elastic Compute Cloud (EC2). The RECOVER Client is an Adobe Flex web map application that is able to provide a suite of convenient GIS analytical capabilities. In a typical use scenario, the RECOVER Server is provided a wildfire name and geospatial extent. The Server then automatically gathers Earth observational data and other relevant products from various geographically distributed data sources. The Server creates a database in the cloud where all relevant information about the wildfire is stored. This information is made available to the RECOVER Client and ultimately to fire managers through their choice of web browser. The Server refreshes the data throughout the burn and subsequent recovery period (3-5 years) with each refresh requiring two minutes to complete. Since remediation plans must be completed within 14 days of a fire's containment, RECOVER has the potential to significantly improve the decision-making process. RECOVER adds an important new dimension to post-fire decision-making by focusing on ecosystem rehabilitation in semiarid savannas. A novel aspect of RECOVER's approach involves the use of soil moisture estimates, which are an important but difficult-to-obtain element of post-fire rehabilitation planning. We will use downscaled soil moisture data from three primary observational sources to begin evaluation of soil moisture products and build the technology needed for RECOVER to use future SMAP products. As a result, RECOVER, BLM, and the fire applications community will be ready customers for data flowing out of new NASA missions, such as NPP, LDCM, and SMAP.

BLM promotes the activation of Fanconi Anemia signaling pathway.

PubMed

Panneerselvam, Jayabal; Wang, Hong; Zhang, Jun; Che, Raymond; Yu, Herbert; Fei, Peiwen

2016-05-31

Mutations in the human RecQ helicase, BLM, causes Bloom Syndrome, which is a rare autosomal recessive disorder and characterized by genomic instability and an increased risk of cancer. Fanconi Anemia (FA), resulting from mutations in any of the 19 known FA genes and those yet to be known, is also characterized by chromosomal instability and a high incidence of cancer. BLM helicase and FA proteins, therefore, may work in a common tumor-suppressor signaling pathway. To date, it remains largely unclear as to how BLM and FA proteins work concurrently in the maintenance of genome stability. Here we report that BLM is involved in the early activation of FA group D2 protein (FANCD2). We found that FANCD2 activation is substantially delayed and attenuated in crosslinking agent-treated cells harboring deficient Blm compared to similarly treated control cells with sufficient BLM. We also identified that the domain VI of BLM plays an essential role in promoting FANCD2 activation in cells treated with DNA crosslinking agents, especially ultraviolet B. The similar biological effects performed by ΔVI-BLM and inactivated FANCD2 further confirm the relationship between BLM and FANCD2. Mutations within the domain VI of BLM detected in human cancer samples demonstrate the functional importance of this domain, suggesting human tumorigenicity resulting from mtBLM may be at least partly attributed to mitigated FANCD2 activation. Collectively, our data show a previously unknown regulatory liaison in advancing our understanding of how the cancer susceptibility gene products act in concert to maintain genome stability.

Blm10 facilitates nuclear import of proteasome core particles

PubMed Central

Weberruss, Marion H; Savulescu, Anca F; Jando, Julia; Bissinger, Thomas; Harel, Amnon; Glickman, Michael H; Enenkel, Cordula

2013-01-01

Short-lived proteins are degraded by proteasome complexes, which contain a proteolytic core particle (CP) but differ in the number of regulatory particles (RPs) and activators. A recently described member of conserved proteasome activators is Blm10. Blm10 contains 32 HEAT-like modules and is structurally related to the nuclear import receptor importin/karyopherin β. In proliferating yeast, RP-CP assemblies are primarily nuclear and promote cell division. During quiescence, RP-CP assemblies dissociate and CP and RP are sequestered into motile cytosolic proteasome storage granuli (PSG). Here, we show that CP sequestration into PSG depends on Blm10, whereas RP sequestration into PSG is independent of Blm10. PSG rapidly clear upon the resumption of cell proliferation and proteasomes are relocated into the nucleus. Thereby, Blm10 facilitates nuclear import of CP. Blm10-bound CP serves as an import receptor–cargo complex, as Blm10 mediates the interaction with FG-rich nucleoporins and is dissociated from the CP by Ran-GTP. Thus, Blm10 represents the first CP-dedicated nuclear import receptor in yeast. PMID:23982732

43 CFR 3253.11 - Must I notify BLM when I have completed my exploration operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL...) Complete any geophysical exploration operations; (b) Complete the drilling of temperature gradient well(s... gradient well; and (d) Plug shot holes and reclaim all exploration sites. ...

Loss of a 20S Proteasome Activator in Saccharomyces cerevisiae Downregulates Genes Important for Genomic Integrity, Increases DNA Damage, and Selectively Sensitizes Cells to Agents With Diverse Mechanisms of Action

PubMed Central

Doherty, Kevin M.; Pride, Leah D.; Lukose, James; Snydsman, Brian E.; Charles, Ronald; Pramanik, Ajay; Muller, Eric G.; Botstein, David; Moore, Carol Wood

2012-01-01

Cytoprotective functions of a 20S proteasome activator were investigated. Saccharomyces cerevisiae Blm10 and human 20S proteasome activator 200 (PA200) are homologs. Comparative genome-wide analyses of untreated diploid cells lacking Blm10 and growing at steady state at defined growth rates revealed downregulation of numerous genes required for accurate chromosome structure, assembly and repair, and upregulation of a specific subset of genes encoding protein-folding chaperones. Blm10 loss or truncation of the Ubp3/Blm3 deubiquitinating enzyme caused massive chromosomal damage and cell death in homozygous diploids after phleomycin treatments, indicating that Blm10 and Ubp3/Blm3 function to stabilize the genome and protect against cell death. Diploids lacking Blm10 also were sensitized to doxorubicin, hydroxyurea, 5-fluorouracil, rapamycin, hydrogen peroxide, methyl methanesulfonate, and calcofluor. Fluorescently tagged Blm10 localized in nuclei, with enhanced fluorescence after DNA replication. After DNA damage that caused a classic G2/M arrest, fluorescence remained diffuse, with evidence of nuclear fragmentation in some cells. Protective functions of Blm10 did not require the carboxyl-terminal region that makes close contact with 20S proteasomes, indicating that protection does not require this contact or the truncated Blm10 can interact with the proteasome apart from this region. Without its carboxyl-terminus, Blm10(−339aa) localized to nuclei in untreated, nonproliferating (G0) cells, but not during G1 S, G2, and M. The results indicate Blm10 functions in protective mechanisms that include the machinery that assures proper assembly of chromosomes. These essential guardian functions have implications for ubiquitin-independent targeting in anticancer therapy. Targeting Blm10/PA200 together with one or more of the upregulated chaperones or a conventional treatment could be efficacious. PMID:22908043

PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution

PubMed Central

Ke, Yuwen; Huh, Jae-Wan; Warrington, Ross; Li, Bing; Wu, Nan; Leng, Mei; Zhang, Junmei; Ball, Haydn L; Li, Bing; Yu, Hongtao

2011-01-01

Centromeres nucleate the formation of kinetochores and are vital for chromosome segregation during mitosis. The SNF2 family helicase PICH (Plk1-interacting checkpoint helicase) and the BLM (the Bloom's syndrome protein) helicase decorate ultrafine histone-negative DNA threads that link the segregating sister centromeres during anaphase. The functions of PICH and BLM at these threads are not understood, however. Here, we show that PICH binds to BLM and enables BLM localization to anaphase centromeric threads. PICH- or BLM-RNAi cells fail to resolve these threads in anaphase. The fragmented threads form centromeric-chromatin-containing micronuclei in daughter cells. Anaphase threads in PICH- and BLM-RNAi cells contain histones and centromere markers. Recombinant purified PICH has nucleosome remodelling activities in vitro. We propose that PICH and BLM unravel centromeric chromatin and keep anaphase DNA threads mostly free of nucleosomes, thus allowing these threads to span long distances between rapidly segregating centromeres without breakage and providing a spatiotemporal window for their resolution. PMID:21743438

43 CFR 3274.11 - What must I give BLM to approve my commercial use permit application?

Code of Federal Regulations, 2014 CFR

2014-10-01

... configuration, including meters; (f) A schematic flow diagram of the utilization facility, including... calibration schedule of production, injection, and royalty meters; (b) A schematic diagram of the utilization... is located off the utilization site, give us a generalized schematic diagram of the electrical...

43 CFR 3274.11 - What must I give BLM to approve my commercial use permit application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... configuration, including meters; (f) A schematic flow diagram of the utilization facility, including... calibration schedule of production, injection, and royalty meters; (b) A schematic diagram of the utilization... is located off the utilization site, give us a generalized schematic diagram of the electrical...

43 CFR 3274.11 - What must I give BLM to approve my commercial use permit application?

Code of Federal Regulations, 2011 CFR

2011-10-01

... configuration, including meters; (f) A schematic flow diagram of the utilization facility, including... calibration schedule of production, injection, and royalty meters; (b) A schematic diagram of the utilization... is located off the utilization site, give us a generalized schematic diagram of the electrical...

43 CFR 3274.11 - What must I give BLM to approve my commercial use permit application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... configuration, including meters; (f) A schematic flow diagram of the utilization facility, including... calibration schedule of production, injection, and royalty meters; (b) A schematic diagram of the utilization... is located off the utilization site, give us a generalized schematic diagram of the electrical...

77 FR 25165 - Environmental Impacts Statements; Notice of Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

.... EIS No. 20120117, Draft EIS, BLM, WY, Lost Creek In Situ Recovery Project, To Analyze the Site... agencies to participate in its e-NEPA electronic EIS submission pilot. Participating agencies can fulfill... filing documents online and providing feedback on the process. To participate in the pilot, register at...

Exploring Pacific Northwest ecosystem resilience: packaging climate change science for federal managers

NASA Astrophysics Data System (ADS)

Bachelet, D. M.

2014-12-01

Climate change is projected to jeopardize ecosystems in the Pacific Northwest. Managing ecosystems for future resilience requires collaboration, innovation and communication. The abundance of data and documents describing the uncertainty around both climate change projections and impacts has become challenging to managers who have little funding and limited time to digest and incorporate these materials into planning and implementation documents. We worked with US Forest Service and BLM managers to help them develop vulnerability assessments and identify on-the-ground strategies to address climate change challenges on the federal lands in northwest Oregon (Siuslaw, Willamette and Mt. Hood National Forests; Eugene and Salem BLM Districts). We held workshops to promote dialogue about climate change, which were particularly effective in fostering discussions between the managers who often do not have the time to share their knowledge and compare experiences across administrative boundaries. We used the Adaptation for Conservation Targets (ACT) framework to identify measurable management objectives and rapidly assess local vulnerabilities. We used databasin.org to centralize usable information, including state-of-the-art CMIP5 climate projections, for the mandated assessments of vulnerability and resilience. We introduced participants to a decision support framework providing opportunities to develop more effective adaptation strategies. We built a special web page to hold the information gathered at the workshops and provide easy access to climate change information. We are now working with several Landscape Conservation Cooperatives (LCCs) to design gateways - conservation atlases - to their relevant data repositories on databasin.org and working with them to develop web tools that can provide usable information for their own vulnerability assessments.

DISTANT VIEW, BLM TACK SHED ON LEFT, BLM SEED SHED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DISTANT VIEW, BLM TACK SHED ON LEFT, BLM SEED SHED AT LEFT CENTER, FIRE DISPATCH OFFICES 1 AND 2 AT RIGHT CENTER, UTILITY BUILDING "B" ON RIGHT. VIEW TO SOUTHWEST. - Cedar City Automotive Repair Shop, 820 North Main Street, Cedar City, Iron County, UT

BLM and RMI1 alleviate RPA inhibition of TopoIIIα decatenase activity.

PubMed

Yang, Jay; Bachrati, Csanad Z; Hickson, Ian D; Brown, Grant W

2012-01-01

RPA is a single-stranded DNA binding protein that physically associates with the BLM complex. RPA stimulates BLM helicase activity as well as the double Holliday junction dissolution activity of the BLM-topoisomerase IIIα complex. We investigated the effect of RPA on the ssDNA decatenase activity of topoisomerase IIIα. We found that RPA and other ssDNA binding proteins inhibit decatenation by topoisomerase IIIα. Complex formation between BLM, TopoIIIα, and RMI1 ablates inhibition of decatenation by ssDNA binding proteins. Together, these data indicate that inhibition by RPA does not involve species-specific interactions between RPA and BLM-TopoIIIα-RMI1, which contrasts with RPA modulation of double Holliday junction dissolution. We propose that topoisomerase IIIα and RPA compete to bind to single-stranded regions of catenanes. Interactions with BLM and RMI1 enhance toposiomerase IIIα activity, promoting decatenation in the presence of RPA.

43 CFR 2804.19 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How will BLM process my Processing... process my Processing Category 6 application? (a) For Processing Category 6 applications, you and BLM must enter into a written agreement that describes how BLM will process your application. The final agreement...

43 CFR 2804.19 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM process my Processing... process my Processing Category 6 application? (a) For Processing Category 6 applications, you and BLM must enter into a written agreement that describes how BLM will process your application. The final agreement...

Up-regulation of antioxidant enzymes and coenzyme Q(10) in a human oral cancer cell line with acquired bleomycin resistance.

PubMed

Yen, Hsiu-Chuan; Li, Sin-Hua; Majima, Hideyuki J; Huang, Yu-Hsiang; Chen, Chiu-Ping; Liu, Chia-Chi; Tu, Ya-Chi; Chen, Chih-Wei

2011-06-01

Bleomycin (BLM) is an anti-cancer drug that can induce formation of reactive oxygen species (ROS). To investigate the association between up-regulation of antioxidant enzymes and coenzyme Q(10) (CoQ(10)) in acquired BLM resistance, one BLM-resistant clone, SBLM24 clone, was selected from a human oral cancer cell line, SCC61 clone. The BLM resistance of SBLM24 clone relative to a sub-clone of SCC61b cells was confirmed by analysis of clonogenic ability and cell cycle arrest. CoQ(10) levels and levels of Mn superoxide dismutase, glutathione peroxidase 1, catalase and thioredoxin reductase 1 were augmented in SBLM24 clone although there was also a mild increase in the expression of BLM hydrolase. Suppression of CoQ(10) levels by 4-aminobenzoate sensitized BLM-induced cytotoxicity. The results of suppression on enhanced ROS production by BLM and the cross-resistance to hydrogen peroxide in SBLM24 clone further demonstrated the development of adaptation to oxidative stress during the formation of acquired BLM resistance.

Interaction between the helicases genetically linked to Fanconi anemia group J and Bloom's syndrome

PubMed Central

Suhasini, Avvaru N; Rawtani, Nina A; Wu, Yuliang; Sommers, Joshua A; Sharma, Sudha; Mosedale, Georgina; North, Phillip S; Cantor, Sharon B; Hickson, Ian D; Brosh, Robert M

2011-01-01

Bloom's syndrome (BS) and Fanconi anemia (FA) are autosomal recessive disorders characterized by cancer and chromosomal instability. BS and FA group J arise from mutations in the BLM and FANCJ genes, respectively, which encode DNA helicases. In this work, FANCJ and BLM were found to interact physically and functionally in human cells and co-localize to nuclear foci in response to replication stress. The cellular level of BLM is strongly dependent upon FANCJ, and BLM is degraded by a proteasome-mediated pathway when FANCJ is depleted. FANCJ-deficient cells display increased sister chromatid exchange and sensitivity to replication stress. Expression of a FANCJ C-terminal fragment that interacts with BLM exerted a dominant negative effect on hydroxyurea resistance by interfering with the FANCJ–BLM interaction. FANCJ and BLM synergistically unwound a DNA duplex substrate with sugar phosphate backbone discontinuity, but not an ‘undamaged' duplex. Collectively, the results suggest that FANCJ catalytic activity and its effect on BLM protein stability contribute to preservation of genomic stability and a normal response to replication stress. PMID:21240188

Malheur River Wildlife Mitigation Project : 2008 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kesling, Jason; Abel, Chad; Schwabe, Laurence

2009-01-01

In 1998, the Burns Paiute Tribe (BPT) submitted a proposal to Bonneville Power Administration (BPA) for the acquisition of the Malheur River Wildlife Mitigation Project (Project). The proposed mitigation site was for the Denny Jones Ranch and included Bureau of Land Management (BLM) and Oregon Division of State Lands (DSL) leases and grazing allotments. The Project approval process and acquisition negotiations continued for several years until the BPT and BPA entered into a Memorandum of Agreement, which allowed for purchase of the Project in November 2000. The 31,781 acre Project is located seven miles east of Juntura, Oregon and ismore » adjacent to the Malheur River (Figure 1). Six thousand three hundred eighty-five acres are deeded to BPT, 4,154 acres are leased from DSL, and 21,242 acres are leased from BLM (Figure 2). In total 11 grazing allotments are leased between the two agencies. Deeded land stretches for seven miles along the Malheur River. It is the largest private landholding on the river between Riverside and Harper, Oregon. Approximately 938 acres of senior water rights are included with the Ranch. The Project is comprised of meadow, wetland, riparian and shrub-steppe habitats. The BLM grazing allotment, located south of the ranch, is largely shrub-steppe habitat punctuated by springs and seeps. Hunter Creek, a perennial stream, flows through both private and BLM lands. Similarly, the DSL grazing allotment, which lies north of the Ranch, is predominantly shrub/juniper steppe habitat with springs and seeps dispersed throughout the upper end of draws (Figure 2).« less

Review of samples of sediment, tailings, and waters adjacent to the Cactus Queen gold mine, Kern County, California

USGS Publications Warehouse

Rytuba, James J.; Kim, Christopher S.; Goldstein, Daniel N.

2011-01-01

The Cactus Queen Mine is located in the western Mojave Desert in Kern County, California. The Cactus Queen gold-silver (Au-Ag) deposit is similar to other Au-Ag deposits hosted in Miocene volcanic rocks that consist of silicic domes and associated flows, pyroclastic rocks, and subvolcanic intrusions. The volcanic rocks were emplaced onto a basement of Mesozoic silicic intrusive rocks. A part of the Cactus Queen Mine is located on Federal land managed by the U.S. Bureau of Land Management (BLM). Staff from the BLM initially sampled the mine area and documented elevated concentrations of arsenic (As) in tailings and sediment. BLM then requested that the U.S. Geological Survey (USGS), in collaboration with Chapman University, measure and characterize As and other geochemical constituents in sediment, tailings, and waters on the part of the mine on Federal lands. This report is made in response to the request by the BLM, the lead agency mandated to conduct a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) - Removal Site Investigation (RSI). The RSI applies to the potential removal of As-contaminated mine waste from the Cactus Queen Mine as a means of reducing As release and exposure to humans and biota. This report summarizes data obtained from field sampling of sediments, mine tailings, and surface waters at the Cactus Queen Mine on January 27, 2008. Our results provide a preliminary assessment of the sources of As and associated chemical constituents that could potentially impact humans and biota.

43 CFR 3809.5 - How does BLM define certain terms used in this subpart?

Code of Federal Regulations, 2011 CFR

2011-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINING CLAIMS... may determine that it is practical to avoid or eliminate particular impacts. Mining claim means any unpatented mining claim, millsite, or tunnel site located under the mining laws. The term also applies to...

43 CFR 3809.5 - How does BLM define certain terms used in this subpart?

Code of Federal Regulations, 2012 CFR

2012-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINING CLAIMS... may determine that it is practical to avoid or eliminate particular impacts. Mining claim means any unpatented mining claim, millsite, or tunnel site located under the mining laws. The term also applies to...

43 CFR 3809.5 - How does BLM define certain terms used in this subpart?

Code of Federal Regulations, 2013 CFR

2013-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINING CLAIMS... may determine that it is practical to avoid or eliminate particular impacts. Mining claim means any unpatented mining claim, millsite, or tunnel site located under the mining laws. The term also applies to...

43 CFR 3809.5 - How does BLM define certain terms used in this subpart?

Code of Federal Regulations, 2014 CFR

2014-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINING CLAIMS... may determine that it is practical to avoid or eliminate particular impacts. Mining claim means any unpatented mining claim, millsite, or tunnel site located under the mining laws. The term also applies to...

43 CFR 2885.15 - How will BLM charge me rent?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM charge me rent? 2885.15 Section 2885.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... LEASING ACT Terms and Conditions of MLA Grants and TUPs § 2885.15 How will BLM charge me rent? (a) BLM...

43 CFR 2806.11 - How will BLM charge me rent?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM charge me rent? 2806.11 Section 2806.11 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... POLICY MANAGEMENT ACT Rents General Provisions § 2806.11 How will BLM charge me rent? (a) BLM will charge...

43 CFR 2806.11 - How will BLM charge me rent?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How will BLM charge me rent? 2806.11 Section 2806.11 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... POLICY MANAGEMENT ACT Rents General Provisions § 2806.11 How will BLM charge me rent? (a) BLM will charge...

43 CFR 2885.15 - How will BLM charge me rent?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How will BLM charge me rent? 2885.15 Section 2885.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND... LEASING ACT Terms and Conditions of MLA Grants and TUPs § 2885.15 How will BLM charge me rent? (a) BLM...

43 CFR 3203.14 - How will BLM provide notice of a competitive lease sale?

Code of Federal Regulations, 2011 CFR

2011-10-01

... before conducting a competitive lease sale, BLM will post the Notice in the BLM office having... having jurisdiction over any of the included lands. (c) BLM may take other measures of notification for... sale; (3) Publishing notice in the newspaper; or (4) Posting the list of parcels on the Internet. ...

43 CFR 3217.14 - When will BLM approve my drilling or development contract?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false When will BLM approve my drilling or... RESOURCE LEASING Cooperative Agreements § 3217.14 When will BLM approve my drilling or development contract? BLM may approve a drilling or development contract when: (a) One or more geothermal lessees enter into...

43 CFR 3217.14 - When will BLM approve my drilling or development contract?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When will BLM approve my drilling or... RESOURCE LEASING Cooperative Agreements § 3217.14 When will BLM approve my drilling or development contract? BLM may approve a drilling or development contract when: (a) One or more geothermal lessees enter into...

76 FR 66958 - Notice of Availability of the Supplement to the Draft Programmatic Environmental Impact Statement...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-28

... energy development on BLM-administered lands. The elements of the BLM's proposed Solar Energy Program.... Amendment of BLM land use plans in the six-state study area to adopt those elements of the new Solar Energy... development, the BLM also proposes a process that will accommodate responsible development outside of SEZs...

43 CFR 2884.17 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How will BLM process my Processing...-WAY UNDER THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.17 How will BLM process my... written agreement that describes how BLM will process your application. The final agreement consists of a...

43 CFR 2884.17 - How will BLM process my Processing Category 6 application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How will BLM process my Processing...-WAY UNDER THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.17 How will BLM process my... written agreement that describes how BLM will process your application. The final agreement consists of a...

Summary of environmental geologic studies in the Mid-Atlantic outer continental shelf area; results of 1978-1979 field seasons

USGS Publications Warehouse

Robb, James M.

1983-01-01

Because of the need for knowledge of an offshore area that is undergoing exploration for oil and gas resources, since 1975 the U.S. Bureau of Land Management (BLM) has funded studies of the environmental characteristics of the Mid-Atlantic Outer Continental Shelf. This volume briefly summarizes a final report to the BLM on the results of U.S. Geological Survey investigations stemming from data acquired during 1978 and 1979. The parent final report contains complete accounts of those investigations. The subjects of the studies range from the geologic effects of water currents and their capabilities of erosion and transportation, to delineation of potentially hazardous geologic characteristics of the area. Nine specific studies address the complexities of water currents, the nature of materials suspended in the sea waters, rates of mixing-in of material deposited on the bottom, and the sites of probable deposition of such materials, as well as sites and mechanisms of possible submarine landsliding or unstable bottom (engineering characteristics) of the Continental Slope and shelf.

Somatic frameshift mutations in the Bloom syndrome BLM gene are frequent in sporadic gastric carcinomas with microsatellite mutator phenotype

PubMed Central

Calin, George; Ranzani, Guglielmina N; Amadori, Dino; Herlea, Vlad; Matei, Irina; Barbanti-Brodano, Giuseppe; Negrini, Massimo

2001-01-01

Background Genomic instability has been reported at microsatellite tracts in few coding sequences. We have shown that the Bloom syndrome BLM gene may be a target of microsatelliteinstability (MSI) in a short poly-adenine repeat located in its coding region. To further characterize the involvement of BLM in tumorigenesis, we have investigated mutations in nine genes containing coding microsatellites in microsatellite mutator phenotype (MMP) positive and negative gastric carcinomas (GCs). Methods We analyzed 50 gastric carcinomas (GCs) for mutations in the BLM poly(A) tract aswell as in the coding microsatellites of the TGFβ1-RII, IGFIIR, hMSH3, hMSH6, BAX, WRN, RECQL and CBL genes. Results BLM mutations were found in 27% of MMP+ GCs (4/15 cases) but not in any of the MMP negative GCs (0/35 cases). The frequency of mutations in the other eight coding regions microsatellite was the following: TGFβ1-RII (60 %), BAX (27%), hMSH6 (20%),hMSH3 (13%), CBL (13%), IGFIIR (7%), RECQL (0%) and WRN (0%). Mutations in BLM appear to be more frequently associated with frameshifts in BAX and in hMSH6and/or hMSH3. Tumors with BLM alterations present a higher frequency of unstable mono- and trinucleotide repeats located in coding regions as compared with mutator phenotype tumors without BLM frameshifts. Conclusions BLM frameshifts are frequent alterations in GCs specifically associated with MMP+tumors. We suggest that BLM loss of function by MSI may increase the genetic instability of a pre-existent unstable genotype in gastric tumors. PMID:11532193

Telomere and ribosomal DNA repeats are chromosomal targets of the bloom syndrome DNA helicase

PubMed Central

Schawalder, James; Paric, Enesa; Neff, Norma F

2003-01-01

Background Bloom syndrome is one of the most cancer-predisposing disorders and is characterized by genomic instability and a high frequency of sister chromatid exchange. The disorder is caused by loss of function of a 3' to 5' RecQ DNA helicase, BLM. The exact role of BLM in maintaining genomic integrity is not known but the helicase has been found to associate with several DNA repair complexes and some DNA replication foci. Results Chromatin immunoprecipitation of BLM complexes recovered telomere and ribosomal DNA repeats. The N-terminus of BLM, required for NB localization, is the same as the telomere association domain of BLM. The C-terminus is required for ribosomal DNA localization. BLM localizes primarily to the non-transcribed spacer region of the ribosomal DNA repeat where replication forks initiate. Bloom syndrome cells expressing the deletion alleles lacking the ribosomal DNA and telomere association domains have altered cell cycle populations with increased S or G2/M cells relative to normal. Conclusion These results identify telomere and ribosomal DNA repeated sequence elements as chromosomal targets for the BLM DNA helicase during the S/G2 phase of the cell cycle. BLM is localized in nuclear bodies when it associates with telomeric repeats in both telomerase positive and negative cells. The BLM DNA helicase participates in genomic stability at ribosomal DNA repeats and telomeres. PMID:14577841

Intermittent Hypoxia Increases the Severity of Bleomycin-Induced Lung Injury in Mice.

PubMed

Gille, Thomas; Didier, Morgane; Rotenberg, Cécile; Delbrel, Eva; Marchant, Dominique; Sutton, Angela; Dard, Nicolas; Haine, Liasmine; Voituron, Nicolas; Bernaudin, Jean-François; Valeyre, Dominique; Nunes, Hilario; Besnard, Valérie; Boncoeur, Emilie; Planès, Carole

2018-01-01

Severe obstructive sleep apnea (OSA) with chronic intermittent hypoxia (IH) is common in idiopathic pulmonary fibrosis (IPF). Here, we evaluated the impact of IH on bleomycin- (BLM-) induced pulmonary fibrosis in mice. C57BL/6J mice received intratracheal BLM or saline and were exposed to IH (40 cycles/hour; FiO 2 nadir: 6%; 8 hours/day) or intermittent air (IA). In the four experimental groups, we evaluated (i) survival; (ii) alveolar inflammation, pulmonary edema, lung oxidative stress, and antioxidant enzymes; (iii) lung cell apoptosis; and (iv) pulmonary fibrosis. Survival at day 21 was lower in the BLM-IH group ( p < 0.05). Pulmonary fibrosis was more severe at day 21 in BLM-IH mice, as assessed by lung collagen content ( p = 0.02) and histology. At day 4, BLM-IH mice developed a more severe neutrophilic alveolitis, ( p < 0.001). Lung oxidative stress was observed, and superoxide dismutase and glutathione peroxidase expression was decreased in BLM-IH mice ( p < 0.05 versus BLM-IA group). At day 8, pulmonary edema was observed and lung cell apoptosis was increased in the BLM-IH group. These results show that exposure to chronic IH increases mortality, lung inflammation, and lung fibrosis in BLM-treated mice. This study raises the question of the worsening impact of severe OSA in IPF patients.

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What kind of financial security does BLM...? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to... post a performance bond. (b) BLM may require a performance bond for contracts of less than $2,000. We...

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false What kind of financial security does BLM...? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to... post a performance bond. (b) BLM may require a performance bond for contracts of less than $2,000. We...

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false What kind of financial security does BLM...? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to... post a performance bond. (b) BLM may require a performance bond for contracts of less than $2,000. We...

Assessing the Potential for Renewable Energy on Public Lands

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

2003-02-01

This report represents an initial activity of the Bureau of Land Managements (BLM) proposed National Energy Policy Implementation Plan: identify and evaluate renewable energy resources on federal lands and any limitations on accessing them. Ultimately, BLM will prioritize land-use planning activities to increase industrys development of renewable energy resources. These resources include solar, biomass, geothermal, water, and wind energy. To accomplish this, BLM and the Department of Energys National Renewable Energy Laboratory (NREL) established a partnership to conduct an assessment of renewable energy resources on BLM lands in the western United States. The objective of this collaboration was to identifymore » BLM planning units in the western states with the highest potential for private-sector development of renewable resources. The assessment resulted in the following findings: (1) 63 BLM planning units in nine western states have high potential for one or more renewable energy technologies; and (2) 20 BLM planning units in seven western states have high potential for power production from three or more renewable energy sources. This assessment report provides BLM with information needed to prioritize land-use planning activities on the basis of potential for the development of energy from renewable resources.« less

Influence of inhalation anesthetics on ion transport across a planar bilayer lipid membrane.

PubMed

Hichiri, Kei; Shirai, Osamu; Kano, Kenji

2012-01-01

Ion transport from one aqueous phase (W1) to another (W2) across a planar bilayer lipid membrane (BLM) in the presence of inhalation anesthetics was electrochemically investigated. In the absence of inhalation anesthetics in the BLM system, no ion transport current flowed between W1 and W2 across the BLM. When inhalation anesthetics such as halothane, chloroform, diethyl ether and trichloroethylene were added to the two aqueous phases or the BLM, the ion transport current quite clearly appeared. When the ratio of the concentration of KCl or NaCl in W1 to that in W2 was varied, the zero current potential across the BLM was shifted. By considering the magnitude of the potential shift, we concluded that the ion transport current can be predominantly ascribed to the transport of Cl(-) across the BLM. Since the dielectric constants of these anesthetics are larger than that of the inner hydrophobic domain of the BLM, the concentration of hydrophilic electrolyte ions in the BLM increases with the increase in the dielectric constant of the inner hydrophobic domain caused by addition of these anesthetics. These situations lead to an increase in the ion permeability coefficient.

Implicit and Explicit Racial Attitudes Changed During Black Lives Matter.

PubMed

Sawyer, Jeremy; Gampa, Anup

2018-07-01

Lab-based interventions have been ineffective in changing individuals' implicit racial attitudes for more than brief durations, and exposure to high-status Black exemplars like Obama has proven ineffective in shifting societal-level racial attitudes. Antiracist social movements, however, offer a potential societal-level alternative for reducing racial bias. Racial attitudes were examined before and during Black Lives Matter (BLM) and its high points of struggle with 1,369,204 participants from 2009 to 2016. After controlling for changes in participant demographics, overall implicit attitudes were less pro-White during BLM than pre-BLM, became increasingly less pro-White across BLM, and were less pro-White during most periods of high BLM struggle. Considering changes in implicit attitudes by participant race, Whites became less implicitly pro-White during BLM, whereas Blacks showed little change. Regarding explicit attitudes, Whites became less pro-White and Blacks became less pro-Black during BLM, each moving toward an egalitarian "no preference" position.

Bleomycin in Octaarginine-modified Fusogenic Liposomes Results in Improved Tumor Growth Inhibition

PubMed Central

Koshkaryev, Alexander; Piroyan, Aleksandr; Torchilin, Vladimir P.

2012-01-01

Bleomycin (BLM) is an example of an anticancer drug that should be delivered into cytosol for its efficient therapeutic action. With this in mind, we developed octaarginine (R8)-modified fusogenic DOPE-liposomes (R8-DOPE-BLM). R8-modification dramatically increased (up to 50-fold) the cell-liposome interaction. R8-DOPE-liposomes were internalized via macropinocytosis and did not end up in the lysosomes. R8-DOPE-BLM led to a significantly stronger cell death and DNA damage in vitro relative to all controls. R8-DOPE-BLM demonstrated a prominent anticancer effect in the BALB/c mice bearing 4T1 tumors. Thus, R8-DOPE-BLM provided efficient intracellular delivery of BLM leading to strong tumor growth inhibition in vivo. PMID:22743614

Protective effect of dexpanthenol on bleomycin-induced pulmonary fibrosis in rats.

PubMed

Ermis, Hilal; Parlakpinar, Hakan; Gulbas, Gazi; Vardi, Nigar; Polat, Alaadin; Cetin, Asli; Kilic, Talat; Aytemur, Zeynep Ayfer

2013-12-01

Despite extensive studies, there is no effective treatment currently available other than pirfenidone for idiopathic pulmonary fibrosis. A protective effect of pantothenic acid and its derivatives on cell damage produced by oxygen radicals has been reported, but it has not been tested in bleomycin (BLM)--induced pulmonary fibrosis in rats. Therefore, we aimed to investigate the preventive effect of dexpanthenol (Dxp) on pulmonary fibrosis. Thirty-two rats were assigned to four groups as follows: (1) control group, (2) dexpanthenol (Dxp) group; 500 mg/kg Dxp continued intraperitoneally for 14 days, (3) bleomycin (BLM) group; a single intratracheal injection of BLM (2.5 mg/kg body weight in 0.25-ml phosphate buffered saline), and (4) BLM + Dxp-treated group; 500 mg/kg Dxp was administered 1 h before the intratracheal BLM injection and continued for 14 days i.p. The histopathological grades of lung inflammation and collagen deposition, tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and myeloperoxidase (MPO) were measured. BLM provoked inflammation and collagen deposition (p < 0.0001), with a marked increase in myeloperoxidase (MPO) activity resembling increased inflammatory activity (p < 0.0001), which was prevented by Dxp (p < 0.0001, p = 0.02). BLM reduced tissue activities of SOD, GPx, and CAT compared to controls (p = 0.01, 0.03, 0.009). MDA was increased with BLM (p = 0.003). SOD (p = 0.001) and MDA (p = 0.016) levels were improved in group 4. The CAT levels in the BLM + Dxp group were close to those in the control group (p > 0.05). We showed that Dxp significantly prevents BLM-induced lung fibrosis in rats. Further studies are required to evaluate the role of Dxp in the treatment of lung fibrosis.

Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes

PubMed Central

Cho, Yoon Hee; Lee, Joong Won; Woo, Hae Dong; Lee, Sunyeong; Kim, Yang Jee; Lee, Younghyun; Shin, Sangah; Joung, Hyojee; Chung, Hai Won

2016-01-01

Following one of the world’s largest nuclear accidents, occured at Fukushima, Japan in 2011, a significant scientific effort has focused on minimizing the potential adverse health effects due to radiation exposure. The use of natural dietary antioxidants to reduce the risk of radiation-induced oxidative DNA damage is a simple strategy for minimizing radiation-related cancer rates and improving overall health. The onion is among the richest sources of dietary flavonoids and is an important food for increasing their overall intake. Therefore, we examined the effect of an onion extract on cyto- and geno-toxicity in human lymphocytes treated with bleomycin (BLM), a radiomimetic agent. In addition, we measured the frequency of micronuclei (MN) and DNA damage following treatment with BLM using a cytokinesis-blocked micronucleus assay and a single cell gel electrophoresis assay. We observed a significant increase in cell viability in lymphocytes treated with onion extract then exposed to BLM compared to cells treated with BLM alone. The frequency of BLM induced MN and DNA damage increased in a dose-dependent manner; however, when lymphocytes were pretreated with onion extract (10 and 20 μL/mL), the frequency of BLM-induced MN was decreased at all doses of BLM and DNA damage was decreased at 3 μg/mL of BLM. These results suggest that onion extract may have protective effects against BLM-induced cyto- and genotoxicity in human lymphocytes. PMID:26907305

Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes.

PubMed

Cho, Yoon Hee; Lee, Joong Won; Woo, Hae Dong; Lee, Sunyeong; Kim, Yang Jee; Lee, Younghyun; Shin, Sangah; Joung, Hyojee; Chung, Hai Won

2016-02-19

Following one of the world's largest nuclear accidents, occured at Fukushima, Japan in 2011, a significant scientific effort has focused on minimizing the potential adverse health effects due to radiation exposure. The use of natural dietary antioxidants to reduce the risk of radiation-induced oxidative DNA damage is a simple strategy for minimizing radiation-related cancer rates and improving overall health. The onion is among the richest sources of dietary flavonoids and is an important food for increasing their overall intake. Therefore, we examined the effect of an onion extract on cyto- and geno-toxicity in human lymphocytes treated with bleomycin (BLM), a radiomimetic agent. In addition, we measured the frequency of micronuclei (MN) and DNA damage following treatment with BLM using a cytokinesis-blocked micronucleus assay and a single cell gel electrophoresis assay. We observed a significant increase in cell viability in lymphocytes treated with onion extract then exposed to BLM compared to cells treated with BLM alone. The frequency of BLM induced MN and DNA damage increased in a dose-dependent manner; however, when lymphocytes were pretreated with onion extract (10 and 20 μL/mL), the frequency of BLM-induced MN was decreased at all doses of BLM and DNA damage was decreased at 3 μg/mL of BLM. These results suggest that onion extract may have protective effects against BLM-induced cyto- and genotoxicity in human lymphocytes.

Alleviation of Cu and Pb rhizotoxicities in cowpea (Vigna unguiculata) as related to ion activities at root-cell plasma membrane surface

USDA-ARS?s Scientific Manuscript database

Cations, such as Ca and Mg, are generally thought to alleviate toxicities of trace metals through site-specific competition (as incorporated in the biotic ligand model, BLM). Short term (48 h) experiments were conducted using cowpea (Vigna unguiculata L. Walp.) seedlings in simple nutrient solution...

76 FR 7878 - Notice of Availability of the Final Environmental Impact Statement for the Westside Land...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-11

... infrastructure, and which exclude certain known eligible cultural sites, would be conveyed; approximately 9,740... act of appropriation, for the acquisition of land, and interests in land, in the Worland District (now... access, fish and wildlife habitat, or cultural resources. On February 22, 2005, the BLM published in the...

75 FR 51479 - Notice of Availability of the Final Environmental Impact Statement for the Chevron Energy...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-20

... (EIS) for the Chevron Energy Solutions/Solar Millennium (CESSM), LLC's Blythe Solar Power Plant (BSPP... project and amend the CDCA Plan to prohibit solar energy projects on the project site. The BLM will take..., LVRWB09B2600] Notice of Availability of the Final Environmental Impact Statement for the Chevron Energy...

43 CFR 2886.15 - How is grant or TUP administration affected if the BLM land my grant or TUP encumbers is...

Code of Federal Regulations, 2012 CFR

2012-10-01

... affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of... administration affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of Federal ownership? (a) If there is a proposal to transfer the BLM land your grant or TUP encumbers...

43 CFR 2886.15 - How is grant or TUP administration affected if the BLM land my grant or TUP encumbers is...

Code of Federal Regulations, 2011 CFR

2011-10-01

... affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of... administration affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of Federal ownership? (a) If there is a proposal to transfer the BLM land your grant or TUP encumbers...

43 CFR 2886.15 - How is grant or TUP administration affected if the BLM land my grant or TUP encumbers is...

Code of Federal Regulations, 2013 CFR

2013-10-01

... affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of... administration affected if the BLM land my grant or TUP encumbers is transferred to another Federal agency or out of Federal ownership? (a) If there is a proposal to transfer the BLM land your grant or TUP encumbers...

Transcriptional responses of zebrafish to complex metal mixtures in laboratory studies overestimates the responses observed with environmental water.

PubMed

Pradhan, Ajay; Ivarsson, Per; Ragnvaldsson, Daniel; Berg, Håkan; Jass, Jana; Olsson, Per-Erik

2017-04-15

Metals released into the environment continue to be of concern for human health. However, risk assessment of metal exposure is often based on total metal levels and usually does not take bioavailability data, metal speciation or matrix effects into consideration. The continued development of biological endpoint analyses are therefore of high importance for improved eco-toxicological risk analyses. While there is an on-going debate concerning synergistic or additive effects of low-level mixed exposures there is little environmental data confirming the observations obtained from laboratory experiments. In the present study we utilized qRT-PCR analysis to identify key metal response genes to develop a method for biomonitoring and risk-assessment of metal pollution. The gene expression patterns were determined for juvenile zebrafish exposed to waters from sites down-stream of a closed mining operation. Genes representing different physiological processes including stress response, inflammation, apoptosis, drug metabolism, ion channels and receptors, and genotoxicity were analyzed. The gene expression patterns of zebrafish exposed to laboratory prepared metal mixes were compared to the patterns obtained with fish exposed to the environmental samples with the same metal composition and concentrations. Exposure to environmental samples resulted in fewer alterations in gene expression compared to laboratory mixes. A biotic ligand model (BLM) was used to approximate the bioavailability of the metals in the environmental setting. However, the BLM results were not in agreement with the experimental data, suggesting that the BLM may be overestimating the risk in the environment. The present study therefore supports the inclusion of site-specific biological analyses to complement the present chemical based assays used for environmental risk-assessment. Copyright © 2017 Elsevier B.V. All rights reserved.

Dynamics of the DNA repair proteins WRN and BLM in the nucleoplasm and nucleoli.

PubMed

Bendtsen, Kristian Moss; Jensen, Martin Borch; May, Alfred; Rasmussen, Lene Juel; Trusina, Ala; Bohr, Vilhelm A; Jensen, Mogens H

2014-11-01

We have investigated the mobility of two EGFP-tagged DNA repair proteins, WRN and BLM. In particular, we focused on the dynamics in two locations, the nucleoli and the nucleoplasm. We found that both WRN and BLM use a "DNA-scanning" mechanism, with rapid binding-unbinding to DNA resulting in effective diffusion. In the nucleoplasm WRN and BLM have effective diffusion coefficients of 1.62 and 1.34 μm(2)/s, respectively. Likewise, the dynamics in the nucleoli are also best described by effective diffusion, but with diffusion coefficients a factor of ten lower than in the nucleoplasm. From this large reduction in diffusion coefficient we were able to classify WRN and BLM as DNA damage scanners. In addition to WRN and BLM we also classified other DNA damage proteins and found they all fall into one of two categories. Either they are scanners, similar to WRN and BLM, with very low diffusion coefficients, suggesting a scanning mechanism, or they are almost freely diffusing, suggesting that they interact with DNA only after initiation of a DNA damage response.

Combined cytogenotoxic effects of bee venom and bleomycin on rat lymphocytes: an in vitro study.

PubMed

Abd-Elhakim, Yasmina M; Khalil, Samah R; Awad, Ashraf; Al-Ayadhi, Laila Y

2014-01-01

This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques. Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μg/mL respectively for 24 and 72 hrs. An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects. To determine the predominant type of BV and/or BLM-induced cell death, LDH release assay was employed beside quantitative expression analyses of the apoptosis-related genes (Caspase-3 and Bcl-2). The genotoxic effects of the tested compounds were evaluated via DNA fragmentation assay. The results of these assays demonstrated that BV potentiates BLM-induced cytotoxicity through increased LDH release and diminished cell viability. Nevertheless, BV significantly inhibited the BLM-induced DNA damage. The results verify that BV significantly attenuates the genotoxic effects of BLM on noncancerous isolated rat lymphocytes but does not diminish BLM cytotoxicity.

The effects of sediment and mercury mobilization in the South Yuba River and Humbug Creek Confluence Area, Nevada County, California: Concentrations, speciation, and environmental fate-Part 1: Field characterization

USGS Publications Warehouse

Fleck, Jacob A.; Alpers, Charles N.; Marvin-DiPasquale, Mark; Hothem, Roger L.; Wright, Scott A.; Ellett, Kevin; Beaulieu, Elizabeth; Agee, Jennifer L.; Kakouros, Evangelos; Kieu, Le H.; Eberl, Dennis D.; Blum, Alex E.; May, Jason T.

2011-01-01

Millions of pounds of mercury (Hg) were deposited in the river and stream channels of the Sierra Nevada from placer and hard-rock mining operations in the late 1800s and early 1900s. The resulting contaminated sediments are relatively harmless when buried and isolated from the overlying aquatic environment. The entrained Hg in the sediment constitutes a potential risk to human and ecosystem health should it be reintroduced to the actively cycling portion of the aquatic system, where it can become methylated and subsequently bioaccumulated in the food web. Each year, sediment is mobilized within these fluvial systems during high stormflows, transporting hundreds of tons of Hg-laden sediment downstream. The State of California and resource-management agencies, including the Bureau of Land Management (BLM) and the U.S. Forest Service, are concerned about additional disturbances, such as from suction gold dredging activities, which have the potential to mobilize Hg associated with buried sediment layers elevated in Hg that are otherwise likely to remain buried under normal storm conditions. The BLM initiated a study looking at the feasibility of removing Hg-contaminated sediment at the confluence of the South Yuba River and Humbug Creek in the northern Sierra Nevada of California by using standard suction-dredge technology. Additionally, the California State Water Resources Control Board (SWRCB) supported a comprehensive characterization of the intended dredge site. Together, the BLM and SWRCB supported a comprehensive characterization of Hg contamination at the site and the potential effects of sediment disturbance at locations with historical hydraulic mining debris on downstream environments. The comprehensive study consisted of two primary components: field studies and laboratory experiments. The field component, described in this report, had several study elements: 1) a preliminary, small-scale, in-stream dredge test; 2) comprehensive characterization of grain size distribution, Hg speciation, and mineralogy of bed and suspended sediment; 3) a determination of the past and current sources of sediment in the study area; 4) an assessment of Hg bioaccumulation in the local invertebrate population; and 5) a comparison of potential Hg transport caused 2 by natural storm disturbances with potential Hg mobilization caused by suction dredging as a method of Hg removal at the study site. The laboratory component of the study assessed the potential influenc of the disturbance of Hg-contaminated sediment through experiments designed to simulate in-stream transport, deposition, and potential methylation of Hg, described in a companion report (see Marvin-DiPasquale and others, 2010). Results of the field studies indicate that the fine-grained fraction (silt-clay, less than 0.063 millimeters) contains the greatest concentration of Hg in contaminated sediment. Because the fine-grained fraction is the most susceptible to long-range fluvial transport, disturbance of Hg-contaminated sediment is likely to increase the concentration and load of Hg in downstream waters. The preliminary, small-scale dredge test showed an increase in the concentration of fine particles and Hg in the water column caused by the dredge activity, despite relatively low concentrations of fine particles and Hg (about 300 nanograms per gram) at the dredge site. Characterization of sediment from two test pits and other sites in the vicinity of the confluence of the South Yuba River and Humbug Creek revealed a highly variable distribution of fine- and coarse-grained sediment. The highest levels of Hg contamination (up to 14,000 ng/g) were associated with the fine-grained fraction of sediment from the bedrock contact zone of Pit 2, a horizon which also yielded grains of gold and gold-Hg amalgam. A closed-circuit tank experiment with a venturi dredge at the base of Pit 1, in a gravel bar within the South Yuba River, resulted in fine-grained suspended sediment remaining in sus

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2011 CFR

2011-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2012 CFR

2012-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2014 CFR

2014-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

43 CFR 3715.7-1 - What types of enforcement action can BLM take if I do not meet the requirements of this subpart?

Code of Federal Regulations, 2013 CFR

2013-10-01

... Laws § 3715.7-1 What types of enforcement action can BLM take if I do not meet the requirements of this... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false What types of enforcement action can BLM... necessary to protect health, safety or the environment. (2) BLM will presume that health, safety or the...

Electrochemical characterization of bilayer lipid membrane-semiconductor junctions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, Xiao Kang; Baral, S.; Fendler, J.H.

Three different systems of glyceryl monooleate (GMO), bilayer lipid membrane (BLM) supported semiconductor particles have been prepared and characterized. A single composition of particulate semiconductor deposited only on one side of the BLM constituted system A, two different compositions of particulate semiconductors sequentially deposited on the same side of the BLM represented system B, and two different compositions of particulate semiconductors deposited on the opposite sides of the BLM made up system C.

Web Content Accessibility of Consumer Health Information Web Sites for People with Disabilities: A Cross Sectional Evaluation

PubMed Central

Parmanto, Bambang

2004-01-01

Background The World Wide Web (WWW) has become an increasingly essential resource for health information consumers. The ability to obtain accurate medical information online quickly, conveniently and privately provides health consumers with the opportunity to make informed decisions and participate actively in their personal care. Little is known, however, about whether the content of this online health information is equally accessible to people with disabilities who must rely on special devices or technologies to process online information due to their visual, hearing, mobility, or cognitive limitations. Objective To construct a framework for an automated Web accessibility evaluation; to evaluate the state of accessibility of consumer health information Web sites; and to investigate the possible relationships between accessibility and other features of the Web sites, including function, popularity and importance. Methods We carried out a cross-sectional study of the state of accessibility of health information Web sites to people with disabilities. We selected 108 consumer health information Web sites from the directory service of a Web search engine. A measurement framework was constructed to automatically measure the level of Web Accessibility Barriers (WAB) of Web sites following Web accessibility specifications. We investigated whether there was a difference between WAB scores across various functional categories of the Web sites, and also evaluated the correlation between the WAB and Alexa traffic rank and Google Page Rank of the Web sites. Results We found that none of the Web sites we looked at are completely accessible to people with disabilities, i.e., there were no sites that had no violation of Web accessibility rules. However, governmental and educational health information Web sites do exhibit better Web accessibility than the other categories of Web sites (P < 0.001). We also found that the correlation between the WAB score and the popularity of a Web site is statistically significant (r = 0.28, P < 0.05), although there is no correlation between the WAB score and the importance of the Web sites (r = 0.15, P = 0.111). Conclusions Evaluation of health information Web sites shows that no Web site scrupulously abides by Web accessibility specifications, even for entities mandated under relevant laws and regulations. Government and education Web sites show better performance than Web sites among other categories. Accessibility of a Web site may have a positive impact on its popularity in general. However, the Web accessibility of a Web site may not have a significant relationship with its importance on the Web. PMID:15249268

Supercritical-Carbon Dioxide Fluid Extract from Chrysanthemum indicum Enhances Anti-Tumor Effect and Reduces Toxicity of Bleomycin in Tumor-Bearing Mice

PubMed Central

Yang, Hong-Mei; Sun, Chao-Yue; Liang, Jia-Li; Xu, Lie-Qiang; Zhang, Zhen-Biao; Luo, Dan-Dan; Chen, Han-Bin; Huang, Yong-Zhong; Wang, Qi; Lee, David Yue-Wei; Yuan, Jie; Li, Yu-Cui

2017-01-01

Bleomycin (BLM), a family of anti-tumor drugs, was reported to exhibit severe side effects limiting its usage in clinical treatment. Therefore, finding adjuvants that enhance the anti-tumor effect and reduce the detrimental effect of BLM is a prerequisite. Chrysanthemum indicum, an edible flower, possesses abundant bioactivities; the supercritical-carbon dioxide fluid extract from flowers and buds of C. indicum (CISCFE) have strong anti-inflammatory, anti-oxidant, and lung protective effects. However, the role of CISCFE combined with BLM treatment on tumor-bearing mice remains unclear. The present study aimed to investigate the potential synergistic effect and the underlying mechanism of CISCFE combined with BLM in the treatment of hepatoma 22 (H22) tumor-bearing mice. The results suggested that the oral administration of CISCFE combined with BLM could markedly prolong the life span, attenuate the BLM-induced pulmonary fibrosis, suppress the production of pro-inflammatory cytokines (interleukin-6), tumor necrosis factor-α, activities of myeloperoxidase, and malondiadehyde. Moreover, CISCFE combined with BLM promoted the ascites cell apoptosis, the activities of caspases 3 and 8, and up-regulated the protein expression of p53 and down-regulated the transforming growth factor-β1 by activating the gene expression of miR-29b. Taken together, these results indicated that CISCFE could enhance the anti-cancer activity of BLM and reduce the BLM-induced pulmonary injury in H22 tumor-bearing mice, rendering it as a potential adjuvant drug with chemotherapy after further investigation in the future. PMID:28245556

Optimization of a murine and human tissue model to recapitulate dermal and pulmonary features of systemic sclerosis

PubMed Central

Watanabe, Tomoya; Mlakar, Logan; Heywood, Jonathan; Malaab, Maya; Hoffman, Stanley

2017-01-01

The murine bleomycin (BLM)-induced fibrosis model is the most widely used in systemic sclerosis (SSc) studies. It has been reported that systemic delivery of BLM via continuous diffusion from subcutaneously implanted osmotic minipumps can cause fibrosis of the skin, lungs, and other internal organs. However, the mouse strain, dosage of BLM, administration period, and additional important features differ from one report to the next. In this study, by employing the pump model in C57BL/6J mice, we show a dose-dependent increase in lung fibrosis by day 28 and a transient increase in dermal thickness. Dermal thickness and the level of collagen in skin treated with high-dose BLM was significantly higher than in skin treated with low dose BLM or vehicle. A reduction in the thickness of the adipose layer was noted in both high and low dose groups at earlier time points suggesting that the loss of the fat layer precedes the onset of fibrosis. High-dose BLM also induced dermal fibrosis and increased expression of fibrosis-associated genes ex vivo in human skin, thus confirming and extending the in vivo findings, and demonstrating that a human organ culture model can be used to assess the effect of BLM on skin. In summary, our findings suggest that the BLM pump model is an attractive model to analyze the underlying mechanisms of fibrosis and test the efficacy of potential therapies. However, the choice of mouse strain, duration of BLM administration and dose must be carefully considered when using this model. PMID:28651005

Optimization of a murine and human tissue model to recapitulate dermal and pulmonary features of systemic sclerosis.

PubMed

Watanabe, Tomoya; Nishimoto, Tetsuya; Mlakar, Logan; Heywood, Jonathan; Malaab, Maya; Hoffman, Stanley; Feghali-Bostwick, Carol

2017-01-01

The murine bleomycin (BLM)-induced fibrosis model is the most widely used in systemic sclerosis (SSc) studies. It has been reported that systemic delivery of BLM via continuous diffusion from subcutaneously implanted osmotic minipumps can cause fibrosis of the skin, lungs, and other internal organs. However, the mouse strain, dosage of BLM, administration period, and additional important features differ from one report to the next. In this study, by employing the pump model in C57BL/6J mice, we show a dose-dependent increase in lung fibrosis by day 28 and a transient increase in dermal thickness. Dermal thickness and the level of collagen in skin treated with high-dose BLM was significantly higher than in skin treated with low dose BLM or vehicle. A reduction in the thickness of the adipose layer was noted in both high and low dose groups at earlier time points suggesting that the loss of the fat layer precedes the onset of fibrosis. High-dose BLM also induced dermal fibrosis and increased expression of fibrosis-associated genes ex vivo in human skin, thus confirming and extending the in vivo findings, and demonstrating that a human organ culture model can be used to assess the effect of BLM on skin. In summary, our findings suggest that the BLM pump model is an attractive model to analyze the underlying mechanisms of fibrosis and test the efficacy of potential therapies. However, the choice of mouse strain, duration of BLM administration and dose must be carefully considered when using this model.

Supercritical-Carbon Dioxide Fluid Extract from Chrysanthemum indicum Enhances Anti-Tumor Effect and Reduces Toxicity of Bleomycin in Tumor-Bearing Mice.

PubMed

Yang, Hong-Mei; Sun, Chao-Yue; Liang, Jia-Li; Xu, Lie-Qiang; Zhang, Zhen-Biao; Luo, Dan-Dan; Chen, Han-Bin; Huang, Yong-Zhong; Wang, Qi; Lee, David Yue-Wei; Yuan, Jie; Li, Yu-Cui

2017-02-24

Bleomycin (BLM), a family of anti-tumor drugs, was reported to exhibit severe side effects limiting its usage in clinical treatment. Therefore, finding adjuvants that enhance the anti-tumor effect and reduce the detrimental effect of BLM is a prerequisite. Chrysanthemum indicum , an edible flower, possesses abundant bioactivities; the supercritical-carbon dioxide fluid extract from flowers and buds of C. indicum (CI SCFE ) have strong anti-inflammatory, anti-oxidant, and lung protective effects. However, the role of CI SCFE combined with BLM treatment on tumor-bearing mice remains unclear. The present study aimed to investigate the potential synergistic effect and the underlying mechanism of CI SCFE combined with BLM in the treatment of hepatoma 22 (H22) tumor-bearing mice. The results suggested that the oral administration of CI SCFE combined with BLM could markedly prolong the life span, attenuate the BLM-induced pulmonary fibrosis, suppress the production of pro-inflammatory cytokines (interleukin-6), tumor necrosis factor-α, activities of myeloperoxidase, and malondiadehyde. Moreover, CI SCFE combined with BLM promoted the ascites cell apoptosis, the activities of caspases 3 and 8, and up-regulated the protein expression of p53 and down-regulated the transforming growth factor-β1 by activating the gene expression of miR-29b. Taken together, these results indicated that CI SCFE could enhance the anti-cancer activity of BLM and reduce the BLM-induced pulmonary injury in H22 tumor-bearing mice, rendering it as a potential adjuvant drug with chemotherapy after further investigation in the future.

Range-wide assessment of livestock grazing across the sagebrush biome

USGS Publications Warehouse

Veblen, Kari E.; Pyke, David A.; Jones, Christopher A.; Casazza, Michael L.; Assal, Timothy J.; Farinha, Melissa A.

2011-01-01

Domestic livestock grazing occurs in virtually all sagebrush habitats and is a prominent disturbance factor. By affecting habitat condition and trend, grazing influences the resources required by, and thus, the distribution and abundance of sagebrush-obligate wildlife species (for example, sage-grouse Centrocercus spp.). Yet, the risks that livestock grazing may pose to these species and their habitats are not always clear. Although livestock grazing intensity and associated habitat condition may be known in many places at the local level, we have not yet been able to answer questions about use, condition, and trend at the landscape scale or at the range-wide scale for wildlife species. A great deal of information about grazing use, management regimes, and ecological condition exists at the local level (for individual livestock management units) under the oversight of organizations such as the Bureau of Land Management (BLM). However, the extent, quality, and types of existing data are unknown, which hinders the compilation, mapping, or analysis of these data. Once compiled, these data may be helpful for drawing conclusions about rangeland status, and we may be able to identify relationships between those data and wildlife habitat at the landscape scale. The overall objective of our study was to perform a range-wide assessment of livestock grazing effects (and the relevant supporting data) in sagebrush ecosystems managed by the BLM. Our assessments and analyses focused primarily on local-level management and data collected at the scale of BLM grazing allotments (that is, individual livestock management units). Specific objectives included the following: 1. Identify and refine existing range-wide datasets to be used for analyses of livestock grazing effects on sagebrush ecosystems. 2. Assess the extent, quality, and types of livestock grazing-related natural resource data collected by BLM range-wide (i.e., across allotments, districts and regions). 3. Compile and synthesize recommendations from federal and university rangeland science experts about how BLM might prioritize collection of different types of livestock grazing-related natural resource data. 4. Investigate whether range-wide datasets (Objective 1) could be used in conjunction with remotely sensed imagery to identify across broad scales (a) allotments potentially not meeting BLM Land Health Standards (LHS) and (b) allotments in which unmet standards might be attributable to livestock grazing. Objective 1: We identified four datasets that potentially could be used for analyses of livestock grazing effects on sagebrush ecosystems. First, we obtained the most current spatial data (typically up to 2007, 2008, or 2009) for all BLM allotments and compiled data into a coarse, topologically enforced dataset that delineated grazing allotment boundaries. Second, we obtained LHS evaluation data (as of 2007) for all allotments across all districts and regions; these data included date of most recent evaluation, BLM determinations of whether region-specific standards were met, and whether BLM deemed livestock to have contributed to any unmet standards. Third, we examined grazing records of three types: Actual Use (permittee-reported), Billed Use (BLM-reported), and Permitted Use (legally authorized). Finally, we explored the possibility of using existing Natural Resources Conservation Service (NRCS) Ecological Site Description (ESD) data to make up-to-date estimates of production and forage availability on BLM allotments. Objective 2: We investigated the availability of BLM livestock grazing-related monitoring data and the status of LHS across 310 randomly selected allotments in 13 BLM field offices. We found that, relative to other data types, the most commonly available monitoring data were Actual Use numbers (permittee-reported livestock numbers and season-of-use), followed by Photo Point, forage Utilization, and finally, Vegetation Trend measurement data. Data availability and frequency of data collection varied across allotments and field offices. Analysis of the BLM's LHS data indicated 67 percent of allotments analyzed were meeting standards. For those not meeting standards, livestock were considered the causal factor in 45 percent of cases (about 15 percent of all allotments). Objective 3: We sought input from 42 university and federal rangeland science experts about how best to prioritize rangeland monitoring activities associated with ascertaining livestock impacts on vegetation resources. When we presented a hypothetical scenario to these scientists and asked them to prioritize monitoring activities, the most common response was to measure ground and vegetation cover, a variable that in many cases (10 of 13 field offices sampled) BLM had already identified as a monitoring priority. Experts identified several other traditional (for example, photo points) and emerging approaches (for example, high-resolution aerial photography) to monitoring. Objective 4: We used spatial allotment data (described in Objective 1) and remotely sensed vegetation data (sagebrush cover, herbaceous vegetation cover, litter and bare soil) to assess differences in allotment LHS status ("Not met" vs. "Met"; if "Not met" - livestock-caused vs. not). We then developed logistic regression models, using vegetation variables to predict LHS status of BLM allotments in sagebrush steppe habitats in Wyoming and portions of Montana and Colorado. In general, we found that more consistent data collection at the local level might improve suitability of data for broad-scale analyses of livestock impacts. As is, data collection methodologies varied across field offices and States, and we did not find any local-level monitoring data (Actual Use, Utilization, Vegetation Trend) that had been collected consistently enough over time or space for range-wide analyses. Moreover, continued and improved emphasis on monitoring also may aid local management decisions, particularly with respect to effects of livestock grazing. Rangeland science experts identified ground cover as a high monitoring priority for assessing range condition and emphasized the importance of tracking livestock numbers and grazing dates. Ultimately, the most effective monitoring program may entail both increased data collection effort and the integration of alternative monitoring approaches (for example, remote sensing or monitoring teams). In the course of our study, we identified three additional datasets that could potentially be used for range-wide analyses: spatial allotment boundary data for all BLM allotments range-wide, LHS evaluations of BLM allotments, and livestock use data (livestock numbers and grazing dates). It may be possible to use these spatial datasets to help prioritize monitoring activities over the extensive land areas managed by BLM. We present an example of how we used spatial allotment boundary data and LHS data to test whether remotely sensed vegetation characteristics could be used to predict which allotments met or did not meet LHS. This approach may be further improved by the results of current efforts by BLM to test whether more intensive (higher resolution) LHS assessments more accurately describe land health status. Standardized data collection in more ecologically meaningful land units may improve our ability to use local-level data for broad-scale analyses.

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2011 CFR

2011-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2012 CFR

2012-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2014 CFR

2014-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

43 CFR 3836.25 - What if BLM denies my petition for deferment of assessment work?

Code of Federal Regulations, 2013 CFR

2013-10-01

...) ANNUAL ASSESSMENT WORK REQUIREMENTS FOR MINING CLAIMS Deferring Assessment Work § 3836.25 What if BLM... BLM decision denying the petition in which to pay the maintenance fee to maintain your claim. ...

Analytical results and sample locality map for rock, stream-sediment, and soil samples, Northern and Eastern Coloado Desert BLM Resource Area, Imperial, Riverside, and San Bernardino Counties, California

USGS Publications Warehouse

King, Harley D.; Chaffee, Maurice A.

2000-01-01

INTRODUCTION In 1996-1998 the U.S. Geological Survey (USGS) conducted a geochemical study of the Bureau of Land Management's (BLM) 5.5 million-acre Northern and Eastern Colorado Desert Resource Area (usually referred to as the NECD in this report), Imperial, Riverside, and San Bernardino Counties, southeastern California (figure 1). This study was done in support of the BLM's Coordinated Management Plan for the area. This report presents analytical data from this study. To provide comprehensive coverage of the NECD, we compiled and examined all available geochemical data, in digital form, from previous studies in the area, and made sample-site plots to aid in determining where sample-site coverage and analyses were sufficient, which samples should be re-analyzed, and where additional sampling was needed. Previous investigations conducted in parts of the current study area included the National Uranium Resource Evaluation (NURE) program studies of the Needles and Salton Sea 1? x 2? quadrangles; USGS studies of 12 BLM Wilderness Study Areas (WSAs) (Big Maria Mountains, Chemehuevi Mountains, Chuckwalla Mountains, Coxcomb Mountains, Mecca Hills, Orocopia Mountains, Palen-McCoy, Picacho Peak, Riverside Mountains, Sheephole Valley (also known as Sheep Hole/Cadiz), Turtle Mountains, and Whipple Mountains); and USGS studies in the Needles and El Centro 1? x 2? quadrangles done during the early 1990s as part of a project to identify the regional geochemistry of southern California. Areas where we did new sampling of rocks and stream sediments are mainly in the Chocolate Mountain Aerial Gunnery Range and in Joshua Tree National Park, which extends into the west-central part of the NECD, as shown in figure 1 and figure 2. This report contains analytical data for 132 rock samples and 1,245 stream-sediment samples collected by the USGS, and 362 stream-sediment samples and 189 soil samples collected during the NURE program. All samples are from the Northern and Eastern Colorado Desert BLM Resource Area and vicinity. Included in the 1,245 stream-sediment samples collected by the USGS are 284 samples collected as part of the current study, 817 samples collected as part of investigations of the12 BLM WSAs and re-analyzed for the present study, 45 samples from the Needles 1? X 2? quadrangle, and 99 samples from the El Centro 1? X 2? quadrangle. The NURE stream-sediment and soil samples were re-analyzed as part of the USGS study in the Needles quadrangle. Analytical data for samples from the Chocolate Mountain Aerial Gunnery Range, which is located within the area of the NECD, were previously reported (King and Chaffee, 1999a). For completeness, these results are also included in this report. Analytical data for samples from the area of Joshua Tree National Park that is within the NECD have also been reported (King and Chaffee, 1999b). These results are not included in this report. The analytical data presented here can be used for baseline geochemical, mineral resource, and environmental geochemical studies.

43 CFR 3261.18 - Do I need to file a bond with BLM before I build a well pad or drill a well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Do I need to file a bond with BLM before I...) GEOTHERMAL RESOURCE LEASING Drilling Operations: Getting a Permit § 3261.18 Do I need to file a bond with BLM before I build a well pad or drill a well? Before starting any operation, you must: (a) File with BLM...

Final Base Realignment and Closure (BRAC) Environmental Assessment for Realignment of Nellis Air Force Base

DTIC Science & Technology

2007-03-01

Traffic Control Assigned Airspace ATG Adversary Tactics Group AWACS Airborne Warning and Control System BAQ Bureau of Air Quality BLM Bureau of Land...Department of Interior Actions BLM The BLM manages millions of acres of public lands in southern Nevada which include portions of NTTR and...within NTTR and would not affect BLM lands adjacent to the base. Therefore, there are no cumulative impacts. USFWS Aircraft operate within the

Bleomycin-induced epithelial–mesenchymal transition in sclerotic skin of mice: Possible role of oxidative stress in the pathogenesis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Cheng-Fan, E-mail: zhouchengfan@sohu.com; Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui 230032; Zhou, Deng-Chuan

Epithelial–mesenchymal transition (EMT) derived myofibroblasts are partly responsible for the increased collagen synthesis and deposition that occur in tissue fibrosis; however EMT occurrence in skin fibrosis and its mechanism remain unknown. The aim of this study was to investigate whether epithelial cells undergo EMT and determine the role of oxidative stress in this process. BALB/c mice were subcutaneously injected with bleomycin (BLM) or phosphate buffer saline (PBS) into the shaved back daily for 2, 3, and 4 weeks. Skin collagen deposition was evaluated by histopathology and Western blotting. EMT characteristics in the skin were determined by histopathology and immunofluorescent stainingmore » for E-cadherin and vimentin, which were further evaluated by Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). To investigate the role of oxidative stress in EMT, the antioxidant N-acetylcysteine (NAC) was intraperitoneally (100 mg/kg body weight/day) injected daily for 3 weeks. The epithelial suprabasal cells were detached from the basement membrane zone (BMZ) in the sclerotic skin treated with BLM. Immunofluorescent staining indicated vimentin-positive epithelial cells frequently occurring in the thickened epidermis of BLM-treated mice. Western blotting and RT-PCR showed that the expression of E-cadherin was significantly decreased but that of vimentin significantly increased in the skin treated with BLM. NAC attenuated BLM induced oxidative damage, changes in E-cadherin and vimentin expressions and collagen deposition in the sclerotic skin of mice. This study provides the first evidence that BLM induces the EMT of the epithelial cells superficial to the basement membrane zone in the skin fibrosis. Oxidative stress may contribute, at least in part, to BLM induced EMT and skin fibrosis in mice. - Highlights: • We provided the first evidence that EMT occurred in BLM-induced skin fibrosis. • Epithelial cells superficial to the BMZ underwent EMT in BLM-induced skin fibrosis. • NAC attenuated EMT induction in the process of BLM-induced skin fibrosis. • NAC didn't significantly inhibit TGF-β expression in BLM-induced skin fibrosis.« less

32 CFR 701.102 - Online resources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... online Web site (http://www.privacy.navy.mil). This Web site supplements this subpart and subpart G. It...) Web site (http://www.doncio.navy.mil). This Web site provides detailed guidance on PIAs. (c) DOD's PA Web site (http://www.defenselink.mil/privacy). This Web site is an excellent resource that contains a...

Watershed Restoration Project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Julie Thompson; Betsy Macfarlan

2007-09-27

In 2003, the U.S. Department of Energy issued the Eastern Nevada Landscape Coalition (ENLC) funding to implement ecological restoration in Gleason Creek and Smith Valley Watersheds. This project was made possible by congressionally directed funding that was provided through the US Department of Energy, Energy Efficiency and Renewable Energy, Office of the Biomass Program. The Ely District Bureau of Land Management (Ely BLM) manages these watersheds and considers them priority areas within the Ely BLM district. These three entities collaborated to address the issues and concerns of Gleason Creek and Smith Valley and prepared a restoration plan to improve themore » watersheds’ ecological health and resiliency. The restoration process began with watershed-scale vegetation assessments and state and transition models to focus on restoration sites. Design and implementation of restoration treatments ensued and were completed in January 2007. This report describes the restoration process ENLC undertook from planning to implementation of two watersheds in semi-arid Eastern Nevada.« less

Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: An In Vitro Study

PubMed Central

Abd-Elhakim, Yasmina M.; Khalil, Samah R.; Awad, Ashraf; AL-Ayadhi, Laila Y.

2014-01-01

This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques. Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μg/mL respectively for 24 and 72 hrs. An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects. To determine the predominant type of BV and/or BLM-induced cell death, LDH release assay was employed beside quantitative expression analyses of the apoptosis-related genes (Caspase-3 and Bcl-2). The genotoxic effects of the tested compounds were evaluated via DNA fragmentation assay. The results of these assays demonstrated that BV potentiates BLM-induced cytotoxicity through increased LDH release and diminished cell viability. Nevertheless, BV significantly inhibited the BLM-induced DNA damage. The results verify that BV significantly attenuates the genotoxic effects of BLM on noncancerous isolated rat lymphocytes but does not diminish BLM cytotoxicity. PMID:24822179

78 FR 57877 - Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Maricopa Solar...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-20

... Park Project, a photovoltaic (PV) solar power plant with a planned generating capacity of up to 300...; AZA35927] Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Maricopa Solar... site at http://www.blm.gov/az/st/en/prog/energy/solar/maricopa-solar.html . In order to be included in...

77 FR 58570 - Notice of Intent To Prepare an Environmental Impact Statement for the Proposed Pacific Connector...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-21

... Survey and Manage species within the range of the northern spotted owl. This is a site-specific amendment... Owl Activity Centers on the BLM Roseburg District: The RMP for the Roseburg District would be amended to exempt the Pacific Connector pipeline project from the requirement to retain habitat in Known Owl...

Analysis of pathology department Web sites and practical recommendations.

PubMed

Nero, Christopher; Dighe, Anand S

2008-09-01

There are numerous customers for pathology departmental Web sites, including pathology department staff, clinical staff, residency applicants, job seekers, and other individuals outside the department seeking department information. Despite the increasing importance of departmental Web sites as a means of distributing information, no analysis has been done to date of the content and usage of pathology department Web sites. In this study, we analyzed pathology department Web sites to examine the elements present on each site and to evaluate the use of search technology on these sites. Further, we examined the usage patterns of our own departmental Internet and internet Web sites to better understand the users of pathology Web sites. We reviewed selected departmental pathology Web sites and analyzed their content and functionality. Our institution's departmental pathology Web sites were modified to enable detailed information to be stored regarding users and usage patterns, and that information was analyzed. We demonstrate considerable heterogeneity in departmental Web sites with many sites lacking basic content and search features. In addition, we demonstrate that increasing the traffic of a department's informational Web sites may result in reduced phone inquiries to the laboratory. We propose recommendations for pathology department Web sites to maximize promotion of a department's mission. A departmental pathology Web site is an essential communication tool for all pathology departments, and attention to the users and content of the site can have operational impact.

The Great War: Online Resources.

ERIC Educational Resources Information Center

Duncanson, Bruce

2002-01-01

Presents an annotated bibliography of Web sites about World War I. Includes: (1) general Web sites; (2) Web sites with information during the war; (3) Web sites with information about post-World War I; (4) Web sites that provide photos, sound files of speeches, and propaganda posters; and (5) Web sites with lesson plans. (CMK)

The Ashkenazic Jewish Bloom syndrome mutation blmAsh is present in non-Jewish Americans of Spanish ancestry.

PubMed Central

Ellis, N A; Ciocci, S; Proytcheva, M; Lennon, D; Groden, J; German, J

1998-01-01

Bloom syndrome (BS) is more frequent in the Ashkenazic Jewish population than in any other. There the predominant mutation, referred to as "blmAsh," is a 6-bp deletion and 7-bp insertion at nucleotide position 2281 in the BLM cDNA. Using a convenient PCR assay, we have identified blmAsh on 58 of 60 chromosomes transmitted by Ashkenazic parents to persons with BS. In contrast, in 91 unrelated non-Ashkenazic persons with BS whom we examined, blmAsh was identified only in 5, these coming from Spanish-speaking Christian families from the southwestern United States, Mexico, or El Salvador. These data, along with haplotype analyses, show that blmAsh was independently established through a founder effect in Ashkenazic Jews and in immigrants to formerly Spanish colonies. This striking observation underscores the complexity of Jewish history and demonstrates the importance of migration and genetic drift in the formation of human populations. PMID:9837821

Anti-genotoxic effect of naringin against bleomycin-induced genomic damage in human lymphocytes in vitro.

PubMed

Yilmaz, Dilek; Teksoy, Ozgun; Bilaloglu, Rahmi; Çinkilic, Nilufer

2016-01-01

Naringin is a flavonoid found in grapefruit and other citrus fruits that shows antioxidant activity. The aim of the present study was to determine the anti-genotoxic and protective effects of naringin on the chemotherapeutic/radiomimetic agent bleomycin (BLM) in human blood lymphocyte cultures in vitro using micronucleus test and chromosomal aberrations (CA) assay. We tested the three doses of naringin (1, 2, 3 µg/mL) and a single dose of BLM (20 µg/mL). BLM significantly increased the total CAs and micronucleus frequency at a concentration of 20 µg/mL. Naringin did not show any toxicity in doses of 1, 2, and 3 µg/mL. Combined treatments of BLM and naringin (2 and 3 µg/mL) significantly reduced micronucleus formation. Naringin dose-dependently decreased the total chromosome aberrations frequency induced by BLM. These results indicate that naringin could prevent BLM (20 µg/mL)-induced genotoxicity.

Food and beverage brands that market to children and adolescents on the internet: a content analysis of branded web sites.

PubMed

Henry, Anna E; Story, Mary

2009-01-01

To identify food and beverage brand Web sites featuring designated children's areas, assess marketing techniques present on those industry Web sites, and determine nutritional quality of branded food items marketed to children. Systematic content analysis of food and beverage brand Web sites and nutrient analysis of food and beverages advertised on these Web sites. The World Wide Web. One-hundred thirty Internet Web sites of food and beverage brands with top media expenditures based on the America's Top 2000 Brands section of Brandweek magazine's annual "Superbrands" report. A standardized content analysis rating form to determine marketing techniques used on the food and beverage brand Web sites. Nutritional analysis of food brands was conducted. Of 130 Web sites analyzed, 48% featured designated children's areas. These Web sites featured a variety of Internet marketing techniques, including advergaming on 85% of the Web sites and interactive programs on 92% of the Web sites. Branded spokescharacters and tie-ins to other products were featured on the majority of the Web sites, as well. Few food brands (13%) with Web sites that market to children met the nutrition criteria set by the National Alliance for Nutrition and Activity. Nearly half of branded Web sites analyzed used designated children's areas to market food and beverages to children, 87% of which were of low nutritional quality. Nutrition professionals should advocate the use of advertising techniques to encourage healthful food choices for children.

Stream and River Condition Across the BLM's National System of Public Lands

EPA Science Inventory

Meeting Abstract: The Bureau of Land Management (BLM), in collaboration with the U.S. Environmental Protection Agency, conducted the first ever Western Rivers and Streams Assessment (WRSA), a survey of the condition of BLM streams and rivers throughout the contiguous western U.S...

Development of a laboratory niche Web site.

PubMed

Dimenstein, Izak B; Dimenstein, Simon I

2013-10-01

This technical note presents the development of a methodological laboratory niche Web site. The "Grossing Technology in Surgical Pathology" (www.grossing-technology.com) Web site is used as an example. Although common steps in creation of most Web sites are followed, there are particular requirements for structuring the template's menu on methodological laboratory Web sites. The "nested doll principle," in which one object is placed inside another, most adequately describes the methodological approach to laboratory Web site design. Fragmentation in presenting the Web site's material highlights the discrete parts of the laboratory procedure. An optimally minimal triad of components can be recommended for the creation of a laboratory niche Web site: a main set of media, a blog, and an ancillary component (host, contact, and links). The inclusion of a blog makes the Web site a dynamic forum for professional communication. By forming links and portals, cloud computing opens opportunities for connecting a niche Web site with other Web sites and professional organizations. As an additional source of information exchange, methodological laboratory niche Web sites are destined to parallel both traditional and new forms, such as books, journals, seminars, webinars, and internal educational materials. Copyright © 2013 Elsevier Inc. All rights reserved.

The quality of online antidepressant drug information: an evaluation of English and Finnish language Web sites.

PubMed

Prusti, Marjo; Lehtineva, Susanna; Pohjanoksa-Mäntylä, Marika; Bell, J Simon

2012-01-01

The Internet is a frequently used source of drug information, including among people with mental disorders. Online drug information may be narrow in scope, incomplete, and contain errors of omission. To evaluate the quality of online antidepressant drug information in English and Finnish. Forty Web sites were identified using the search terms antidepressants and masennuslääkkeet in English and Finnish, respectively. Included Web sites (14 English, 8 Finnish) were evaluated for aesthetics, interactivity, content coverage, and content correctness using published criteria. All Web sites were assessed using the Date, Author, References, Type, Sponsor (DARTS) and DISCERN quality assessment tools. English and Finnish Web sites had similar aesthetics, content coverage, and content correctness scores. English Web sites were more interactive than Finnish Web sites (P<.05). Overall, adverse drug reactions were covered on 21 of 22 Web sites; however, drug-alcohol interactions were addressed on only 9 of 22 Web sites, and dose was addressed on only 6 of 22 Web sites. Few (2/22 Web sites) provided incorrect information. The DISCERN score was significantly correlated with content coverage (r=0.670, P<.01), content correctness (r=0.663, P<.01), and the DARTS score (r=0.459, P<.05). No Web site provided information about all aspects of antidepressant treatment. Nevertheless, few Web sites provided incorrect information. Both English and Finnish Web sites were similar in terms of aesthetics, content coverage, and content correctness. Copyright © 2012 Elsevier Inc. All rights reserved.

Meeting Reference Responsibilities through Library Web Sites.

ERIC Educational Resources Information Center

Adams, Michael

2001-01-01

Discusses library Web sites and explains some of the benefits when libraries make their sites into reference portals, linking them to other useful Web sites. Topics include print versus Web information sources; limitations of search engines; what Web sites to include, including criteria for inclusions; and organizing the sites. (LRW)

43 CFR 2802.11 - How does BLM designate corridors?

Code of Federal Regulations, 2013 CFR

2013-10-01

... air, water, soil, fish, wildlife, and vegetation; (3) Physical effects and constraints on corridor... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM designate corridors? 2802.11... POLICY MANAGEMENT ACT Lands Available for FLPMA Grants § 2802.11 How does BLM designate corridors? (a...

43 CFR 2802.11 - How does BLM designate corridors?

Code of Federal Regulations, 2012 CFR

2012-10-01

... air, water, soil, fish, wildlife, and vegetation; (3) Physical effects and constraints on corridor... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM designate corridors? 2802.11... POLICY MANAGEMENT ACT Lands Available for FLPMA Grants § 2802.11 How does BLM designate corridors? (a...

43 CFR 2802.11 - How does BLM designate corridors?

Code of Federal Regulations, 2011 CFR

2011-10-01

... air, water, soil, fish, wildlife, and vegetation; (3) Physical effects and constraints on corridor... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How does BLM designate corridors? 2802.11... POLICY MANAGEMENT ACT Lands Available for FLPMA Grants § 2802.11 How does BLM designate corridors? (a...

76 FR 30739 - Notice of Public Meeting, Salem District Resource Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-26

.... ADDRESSES: BLM Salem District Office, 1717 Fabry Road, SE., Salem, OR 97306. FOR FURTHER INFORMATION CONTACT... Hatfield, BLM Salem District Designated Official, 1717 Fabry Road, SE., Salem, OR 97306--(503) 375-5682... quality, land health, forest ecosystems, and infrastructure. Miles R. Brown, BLM Salem District Manager...

Stream and River Condition Across the BLM's National System of Public Lands.

EPA Science Inventory

The Bureau of Land Management (BLM), in collaboration with the U.S. Environmental Protection Agency, conducted the first ever Western Rivers and Streams Assessment (WRSA), a survey of the condition of BLM streams and rivers throughout the contiguous western U.S. The WRSA was desi...

78 FR 35956 - Utah Resource Advisory Council Subgroup Conference Call

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-14

... BLM-Utah's draft three-year National Conservation Lands Strategy. In May 2013, the RAC provided the BLM-Utah State Director with recommended changes to the draft strategy and this meeting was held to discuss how BLM-Utah has incorporated their recommendations into a revised draft strategy. A public...

43 CFR 3602.42 - How does BLM publicize competitive mineral materials sales?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How does BLM publicize competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.42 How does BLM publicize competitive...

43 CFR 3602.42 - How does BLM publicize competitive mineral materials sales?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM publicize competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.42 How does BLM publicize competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

43 CFR 3602.42 - How does BLM publicize competitive mineral materials sales?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM publicize competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.42 How does BLM publicize competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

43 CFR 3602.43 - How does BLM conduct competitive mineral materials sales?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How does BLM conduct competitive mineral... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.43 How does BLM conduct competitive...

SELF-BLM: Prediction of drug-target interactions via self-training SVM.

PubMed

Keum, Jongsoo; Nam, Hojung

2017-01-01

Predicting drug-target interactions is important for the development of novel drugs and the repositioning of drugs. To predict such interactions, there are a number of methods based on drug and target protein similarity. Although these methods, such as the bipartite local model (BLM), show promise, they often categorize unknown interactions as negative interaction. Therefore, these methods are not ideal for finding potential drug-target interactions that have not yet been validated as positive interactions. Thus, here we propose a method that integrates machine learning techniques, such as self-training support vector machine (SVM) and BLM, to develop a self-training bipartite local model (SELF-BLM) that facilitates the identification of potential interactions. The method first categorizes unlabeled interactions and negative interactions among unknown interactions using a clustering method. Then, using the BLM method and self-training SVM, the unlabeled interactions are self-trained and final local classification models are constructed. When applied to four classes of proteins that include enzymes, G-protein coupled receptors (GPCRs), ion channels, and nuclear receptors, SELF-BLM showed the best performance for predicting not only known interactions but also potential interactions in three protein classes compare to other related studies. The implemented software and supporting data are available at https://github.com/GIST-CSBL/SELF-BLM.

Gas Migration Project: Risk Assessment Tool and Computational Analyses to Investigate Wellbore/Mine Interactions, Secretary's Potash Area, Southeastern New Mexico

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sobolik, Steven R.; Hadgu, Teklu; Rechard, Robert P.

The Bureau of Land Management (BLM), US Department of the Interior has asked Sandia National Laboratories (SNL) to perform scientific studies relevant to technical issues that arise in the development of co-located resources of potash and petroleum in southeastern New Mexico in the Secretary’s Potash Area. The BLM manages resource development, issues permits and interacts with the State of New Mexico in the process of developing regulations, in an environment where many issues are disputed by industry stakeholders. The present report is a deliverable of the study of the potential for gas migration from a wellbore to a mine openingmore » in the event of wellbore leakage, a risk scenario about which there is disagreement among stakeholders and little previous site specific analysis. One goal of this study was to develop a framework that required collaboratively developed inputs and analytical approaches in order to encourage stakeholder participation and to employ ranges of data values and scenarios. SNL presents here a description of a basic risk assessment (RA) framework that will fulfill the initial steps of meeting that goal. SNL used the gas migration problem to set up example conceptual models, parameter sets and computer models and as a foundation for future development of RA to support BLM resource development.« less

BLM Density Management and Riparian Buffer Study: Establishment Report and Study Plan

USGS Publications Warehouse

Cissel, John H.; Anderson, P.D.; Olson, Deanna H.; Puettmann, Klaus; Berryman, Shanti; Chan, Samuel; Thompson, Charley

2006-01-01

The Bureau of Land Management (BLM), Pacific Northwest Research Station (PNW), U.S. Geological Survey (USGS), and Oregon State University (OSU) established the BLM Density Management and Riparian Buffer Study (DMS) in 1994 to demonstrate and test options for young stand management to meet Northwest Forest Plan objectives in western Oregon. The primary objectives of the DMS are to evaluate the effects of alternative forest density management treatments in young stands on the development of important late-successional forest habitat attributes and to assess the combined effects of density management and alternative riparian buffer widths on aquatic and riparian ecosystems. The DMS consists of three integrated studies: initial thinning, rethinning, and riparian buffer widths. The initial thinning study was installed in 50- to 80-year-old stands that had never been commercially thinned. Four stand treatments of 30-60 acres each were established at each of seven study sites: (1) unthinned control, (2) high density retention [120 trees per acre (TPA)], (3) moderate density retention (80 TPA), and (4) variable density retention (40-120 TPA). Small (1/4 to 1 acre in size) leave islands were included in all treatments except the control, and small patch cuts (1/4 to 1 acre in size) were included in the moderate and variable density treatments. An eighth site, Callahan Creek, contains a partial implementation of the study design. The rethinning study was installed in four 70- to 90-year-old stands that previously had been commercially thinned. Each study stand was split into two parts: one part as an untreated control and the other part as a rethinning (30-60 TPA). The riparian buffer study was nested within the moderate density retention treatment at each of the eight initial thinning study sites and two rethinning sites. Alternative riparian buffer widths included: (1) streamside retention (one tree canopy width, or 20-25 feet), (2) variable width (follows topographic and vegetative breaks, 50 feet slope distance minimum), (3) one full site-potential tree height (approximately 220 feet), and (4) two full tree heights (approximately 440 feet). A second round of density management manipulations are now being planned for implementation beginning in 2009. Stem density will be reduced in the high, moderate, and variable density treatments and most existing riparian buffers, leave islands, and patch cuts will remain in place. Remeasurement, data management, and analysis are ongoing for three long-term, core components of the DMS: vegetation, microclimate, and aquatic vertebrates. In addition, several short-term collaborative studies have been completed on these sites, including leave island effectiveness as refugia, treatment response of terrestrial and aquatic arthropods, and smaller-scale studies of fungal, lichen, and bryophyte community response. Additional collaborative studies are encouraged on DMS sites.

Accessibility and content of individualized adult reconstructive hip and knee/musculoskeletal oncology fellowship web sites.

PubMed

Young, Bradley L; Cantrell, Colin K; Patt, Joshua C; Ponce, Brent A

2018-06-01

Accessible, adequate online information is important to fellowship applicants. Program web sites can affect which programs applicants apply to, subsequently altering interview costs incurred by both parties and ultimately impacting rank lists. Web site analyses have been performed for all orthopaedic subspecialties other than those involved in the combined adult reconstruction and musculoskeletal (MSK) oncology fellowship match. A complete list of active programs was obtained from the official adult reconstruction and MSK oncology society web sites. Web site accessibility was assessed using a structured Google search. Accessible web sites were evaluated based on 21 previously reported content criteria. Seventy-four adult reconstruction programs and 11 MSK oncology programs were listed on the official society web sites. Web sites were identified and accessible for 58 (78%) adult reconstruction and 9 (82%) MSK oncology fellowship programs. No web site contained all content criteria and more than half of both adult reconstruction and MSK oncology web sites failed to include 12 of the 21 criteria. Several programs participating in the combined Adult Reconstructive Hip and Knee/Musculoskeletal Oncology Fellowship Match did not have accessible web sites. Of the web sites that were accessible, none contained comprehensive information and the majority lacked information that has been previously identified as being important to perspective applicants.

Development of breast tumors in CHEK2, NBN/NBS1 and BLM mutation carriers does not commonly involve somatic inactivation of the wild-type allele.

PubMed

Suspitsin, Evgeny N; Yanus, Grigory A; Sokolenko, Anna P; Yatsuk, Olga S; Zaitseva, Olga A; Bessonov, Alexandr A; Ivantsov, Alexandr O; Heinstein, Valeria A; Klimashevskiy, Valery F; Togo, Alexandr V; Imyanitov, Evgeny N

2014-02-01

Somatic inactivation of the remaining allele is a characteristic feature of cancers arising in BRCA1 and BRCA2 mutation carriers, which determines their unprecedented sensitivity to some DNA-damaging agents. Data on tumor-specific status of the involved gene in novel varieties of hereditary breast cancer (BC) remain incomplete. We analyzed 32 tumors obtained from 30 patients with non-BRCA1/2 BC-associated germ-line mutations: 25 women were single mutation carriers (7 BLM, 15 CHEK2 and 3 NBN/NBS1) and 5 were double mutation carriers (2 BLM/BRCA1, 1 CHEK2/BLM, 1 CHEK2/BRCA1 and 1 NBN/BLM). Losses of heterozygosity affecting the wild-type allele were detected in none of the tumors from BLM mutation carriers, 3/18 (17 %) CHEK2-associated BC and 1/4 (25 %) NBN/NBS1-driven tumors. The remaining 28 BC were subjected to the sequence analysis of entire coding region of the involved gene; no somatic mutations were identified. We conclude that the tumor-specific loss of the wild-type allele is not characteristic for BC arising in CHEK2, NBN/NBS1 and BLM mutation carriers. Rarity of "second-hit" inactivation of the involved gene in CHEK2-, NBN/NBS1- and BLM-associated BC demonstrates their substantial biological difference from BRCA1/2-driven cancers and makes them poorly suitable for the clinical trials with cisplatin and PARP inhibitors.

A systematic review of patient inflammatory bowel disease information resources on the World Wide Web.

PubMed

Bernard, André; Langille, Morgan; Hughes, Stephanie; Rose, Caren; Leddin, Desmond; Veldhuyzen van Zanten, Sander

2007-09-01

The Internet is a widely used information resource for patients with inflammatory bowel disease, but there is variation in the quality of Web sites that have patient information regarding Crohn's disease and ulcerative colitis. The purpose of the current study is to systematically evaluate the quality of these Web sites. The top 50 Web sites appearing in Google using the terms "Crohn's disease" or "ulcerative colitis" were included in the study. Web sites were evaluated using a (a) Quality Evaluation Instrument (QEI) that awarded Web sites points (0-107) for specific information on various aspects of inflammatory bowel disease, (b) a five-point Global Quality Score (GQS), (c) two reading grade level scores, and (d) a six-point integrity score. Thirty-four Web sites met the inclusion criteria, 16 Web sites were excluded because they were portals or non-IBD oriented. The median QEI score was 57 with five Web sites scoring higher than 75 points. The median Global Quality Score was 2.0 with five Web sites achieving scores of 4 or 5. The average reading grade level score was 11.2. The median integrity score was 3.0. There is marked variation in the quality of the Web sites containing information on Crohn's disease and ulcerative colitis. Many Web sites suffered from poor quality but there were five high-scoring Web sites.

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application... applicable Federal and state laws; (3) If your application is for a road, determine whether it is in the...

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2014 CFR

2014-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application... applicable Federal and state laws; (3) If your application is for a road, determine whether it is in the...

ACHP | News

Science.gov Websites

the agreement will be signed in February 2012. This final draft of the PA enhances the role of tribal governments, consulting parties, and the public in the BLM project review process. It discusses the role of jcordova@blm.gov. For all other questions regarding the PA, BLM's Preservation Officer Robin Hawks should

Facilitating Backcountry Use of Bureau of Land Management Wildlands

Treesearch

R. Steve Smith

1992-01-01

BLM wildlands are extensive areas which offer opportunities for increased backcountry use. Many BLM wildland areas are not currently receiving much backcountry use due to their unfamiliarity by the public and lack of facilities. Increased urban/BLM wildland interfacing can produce important benefits for both individuals and our society. Using various informational...

43 CFR 3503.41 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2012 CFR

2012-10-01

... Department of the Interior covering public disclosure of data and information contained in Department of the Interior records. BLM may make certain mineral information not protected from disclosure under part 2 of... § 3503.41 Will BLM disclose information I submit under these regulations? All Federal and Indian data and...

43 CFR 3503.41 - Will BLM disclose information I submit under these regulations?

Code of Federal Regulations, 2013 CFR

2013-10-01

... Department of the Interior covering public disclosure of data and information contained in Department of the Interior records. BLM may make certain mineral information not protected from disclosure under part 2 of... § 3503.41 Will BLM disclose information I submit under these regulations? All Federal and Indian data and...

43 CFR 3505.61 - May BLM extend the term of my prospecting permit?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false May BLM extend the term of my prospecting....61 May BLM extend the term of my prospecting permit? We may extend prospecting permits for phosphate... additional 2 years. We cannot extend sodium and sulphur prospecting permits. ...

43 CFR 3505.61 - May BLM extend the term of my prospecting permit?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false May BLM extend the term of my prospecting....61 May BLM extend the term of my prospecting permit? We may extend prospecting permits for phosphate... additional 2 years. We cannot extend sodium and sulphur prospecting permits. ...

43 CFR 3505.61 - May BLM extend the term of my prospecting permit?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false May BLM extend the term of my prospecting....61 May BLM extend the term of my prospecting permit? We may extend prospecting permits for phosphate... additional 2 years. We cannot extend sodium and sulphur prospecting permits. ...

ACHP | News | BLM Nationwide Programmatic Agreement Addendum Signed

Science.gov Websites

, determined it should be updated to incorporate further the role of Indian tribes. Through this addendum BLM committed to developing revisions to the PA, in consultation with the signatories and Indian tribes, within developing subsequent implementing actions. The addendum also recognizes that the BLM initiated an outreach

43 CFR 1822.11 - What must I do to make an official filing with BLM?

Code of Federal Regulations, 2011 CFR

2011-10-01

... file your application and any other required documents during regular office hours at the appropriate BLM office having jurisdiction over the lands or records involved. You must file any document with BLM through personal delivery or by mailing via the United States Postal Service or other delivery service...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

43 CFR 3602.41 - When will BLM sell mineral materials on a competitive basis?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false When will BLM sell mineral materials on a... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) MINERAL MATERIALS DISPOSAL Mineral Materials Sales Competitive Sales § 3602.41 When will BLM sell mineral materials...

Bloom syndrome helicase in meiosis: Pro-crossover functions of an anti-crossover protein.

PubMed

Hatkevich, Talia; Sekelsky, Jeff

2017-09-01

The functions of the Bloom syndrome helicase (BLM) and its orthologs are well characterized in mitotic DNA damage repair, but their roles within the context of meiotic recombination are less clear. In meiotic recombination, multiple repair pathways are used to repair meiotic DSBs, and current studies suggest that BLM may regulate the use of these pathways. Based on literature from Saccharomyces cerevisiae, Arabidopsis thaliana, Mus musculus, Drosophila melanogaster, and Caenorhabditis elegans, we present a unified model for a critical meiotic role of BLM and its orthologs. In this model, BLM and its orthologs utilize helicase activity to regulate the use of various pathways in meiotic recombination by continuously disassembling recombination intermediates. This unwinding activity provides the meiotic program with a steady pool of early recombination substrates, increasing the probability for a DSB to be processed by the appropriate pathway. As a result of BLM activity, crossovers are properly placed throughout the genome, promoting proper chromosomal disjunction at the end of meiosis. This unified model can be used to further refine the complex role of BLM and its orthologs in meiotic recombination. © 2017 WILEY Periodicals, Inc.

BLM helicase facilitates telomere replication during leading strand synthesis of telomeres

PubMed Central

Kosiyatrakul, Settapong T.

2015-01-01

Based on its in vitro unwinding activity on G-quadruplex (G4) DNA, the Bloom syndrome–associated helicase BLM is proposed to participate in telomere replication by aiding fork progression through G-rich telomeric DNA. Single molecule analysis of replicated DNA (SMARD) was used to determine the contribution of BLM helicase to telomere replication. In BLM-deficient cells, replication forks initiating from origins within the telomere, which copy the G-rich strand by leading strand synthesis, moved slower through the telomere compared with the adjacent subtelomere. Fork progression through the telomere was further slowed in the presence of a G4 stabilizer. Using a G4-specific antibody, we found that deficiency of BLM, or another G4-unwinding helicase, the Werner syndrome-associated helicase WRN, resulted in increased G4 structures in cells. Importantly, deficiency of either helicase led to greater increases in G4 DNA detected in the telomere compared with G4 seen genome-wide. Collectively, our findings are consistent with BLM helicase facilitating telomere replication by resolving G4 structures formed during copying of the G-rich strand by leading strand synthesis. PMID:26195664

BLM and the FANC proteins collaborate in a common pathway in response to stalled replication forks

PubMed Central

Pichierri, Pietro; Franchitto, Annapaola; Rosselli, Filippo

2004-01-01

Fanconi anaemia (FA) and Bloom syndrome (BS) are autosomal recessive diseases characterised by chromosome fragility and cancer proneness. Here, we report that BLM and the FA pathway are activated in response to both crosslinked DNA and replication fork stall. We provide evidence that BLM and FANCD2 colocalise and co-immunoprecipitate following treatment with either DNA crosslinkers or agents inducing replication arrest. We also find that the FA core complex is necessary for BLM phosphorylation and assembly in nuclear foci in response to crosslinked DNA. Moreover, we show that knock-down of the MRE11 complex, whose function is also under the control of the FA core complex, enhances cellular and chromosomal sensitivity to DNA interstrand crosslinks in BS cells. These findings suggest the existence of a functional link between BLM and the FA pathway and that BLM and the MRE11 complex are in two separated branches of a pathway resulting in S-phase checkpoint activation, chromosome integrity and cell survival in response to crosslinked DNA. PMID:15257300

Reservoir High's TE Site Wins Web Site of the Month

ERIC Educational Resources Information Center

Tech Directions, 2008

2008-01-01

This article features "Mr. Rhine's Technology Education Web Site," a winner of the Web Site of the Month. This Web site was designed by Luke Rhine, a teacher at the Reservoir High School in Fulton, Maryland. Rhine's Web site offers course descriptions and syllabuses, class calendars, lectures and presentations, design briefs and other course…

The Way of the Web: Answers to Your Questions about Web Site Marketing.

ERIC Educational Resources Information Center

Wassom, Julie

2002-01-01

Provides suggestions for effective web site marketing for child care and early education programs. Includes key considerations in designing a web site, specific elements that cause visitors to stay on and return to the site, use of interactive sites, web-site updating and revision, and use of traditional marketing activities to direct prospective…

75 FR 73123 - Notice of Correction to Notice of Availability of the Environmental Assessment and Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-29

...The Bureau of Land Management (BLM) is correcting the Notice of Availability of the Environmental Assessment (EA) and Notice of Public Hearing for the Sage Creek Holdings, LLC, Federal Coal Lease Application, COC-74219 published in the Federal Register on August 13, 2010 [75 FR 49512]. The BLM incorrectly stated that the EA was complete and available for release. The BLM subsequently determined that additional information should have been included in the cumulative impact section of the EA. The BLM will be issuing a revised EA, which will be available for a 30-day comment period upon completion. After the end of the comment period, the BLM will hold a public hearing on the EA, the fair market value and the maximum economic recovery of the proposed leased tract.

Web Site Credibility: Why Do People Believe What They Believe?

ERIC Educational Resources Information Center

Iding, Marie K.; Crosby, Martha E.; Auernheimer, Brent; Klemm, E. Barbara

2009-01-01

This research investigates university students' determinations of credibility of information on Web sites, confidence in their determinations, and perceptions of Web site authors' vested interests. In Study 1, university-level computer science and education students selected Web sites determined to be credible and Web sites that exemplified…

Personality in cyberspace: personal Web sites as media for personality expressions and impressions.

PubMed

Marcus, Bernd; Machilek, Franz; Schütz, Astrid

2006-06-01

This research examined the personality of owners of personal Web sites based on self-reports, visitors' ratings, and the content of the Web sites. The authors compared a large sample of Web site owners with population-wide samples on the Big Five dimensions of personality. Controlling for demographic differences, the average Web site owner reported being slightly less extraverted and more open to experience. Compared with various other samples, Web site owners did not generally differ on narcissism, self-monitoring, or self-esteem, but gender differences on these traits were often smaller in Web site owners. Self-other agreement was highest with Openness to Experience, but valid judgments of all Big Five dimensions were derived from Web sites providing rich information. Visitors made use of quantifiable features of the Web site to infer personality, and the cues they utilized partly corresponded to self-reported traits. Copyright 2006 APA, all rights reserved.

Black Lipid Membranes at Bifaces

PubMed Central

Ti Tien, H.

1968-01-01

Black lipid membranes (BLM) less than 90 A thick have been shown to be the most realistic approach to biological membrane models. This paper describes the formation characteristics, optical properties, and thermodynamics of BLM at water/oil/water bifaces. In particular, the nature of the Plateau-Gibbs border which supports the black membrane is analyzed in some detail. The formation of BLM at the biface involves a spontaneous reduction of the free energy of the system. As long as the integrity of the membrane is maintained, the limiting structure of the BLM represents the lowest free energy configuration. PMID:19873618

[Effect of CsA bleomycin-induced interstitial pulmonary disease in mice].

PubMed

Ren, Ying; Yang, Hui; Zhu, Ping; Fan, Chun-mei; Wang, Yan-hong; Li, Jia; Liu, Hui

2012-03-01

To observe the therapeutic effect of cyclosporine A (CsA) on bleomycin (BLM) induced pulmonary fibrosis and to investigate its mechanism. One hundred and twenty C57BL/6 female mice were divided randomly into five groups: BLM model group, control saline group, CsA30 mg treatment group, CsA50 mg treatment group and control treatment group. Treatment groups and model groups were administrated BLM intratracheally to induce interstitial pulmonary disease model, with control saline group administrated with equal volume of normal saline instead. Mice in treatment groups were intraperitoneal injected with CsA, while control treatment group were injected with equal volume of normal saline instead. On the 4th, 7th and 14th day after administration, 8 mice of each group were sacrificed, and the peripheral blood was obtained to count total leucocytes with counting chamber and quantify CD4(+); T cells, CD14(+); monocytes and CD19(+); B cells by flow cytometry (FCM). Bronchoalveolar levage fluid was harvested for cell counting and Giemsa staining. Lung tissues were harvested for immunohistochemical staining and pathological examination. The quantity of total leucocyte was higher in BLM model group than those in control saline group.The proportion of CD14(+); T cells and CD19(+);B cells in BLM model group were increased markedly than those in control saline group on the 4th, 7th and 14th day post BLM. With CsA treatment, The proportion of CD14(+); T cells was lower than BLM model group at the same time point, especially on the 4th day. The proportion of CD19(+); B cells were significantly lower than those of BLM model group at the same time point(7 d, 14 d). The total and classification of cells of BLM model group were increased markedly than those in control saline group, and decreased obviously in the treatment groups at the same time point. Examination of lung tissues: With the prolonged time of BLM administration, it showed wider alveolar septum, more collagen deposition, as well as more infiltrating inflammatory cells which consisted of generous lymphocyte and few mononuclear macrophages than those in saline control group. With the prolonged time of CsA injection, the interstitial pulmonary inflammation was remissive, and there was less fibroblast infiltration and collagen deposition in pulmonary interstitium and periphery of bronchiole. Alveolar epithelial cells, bronchiolar epithelial cells, mononuclear macrophages, neutrophils and lymphocytes were demonstrated to express CD147, there was higher CD147 expression in BLM model group than those in CsA treatment groups. CsA may heal BLM induced interstitial pulmonary disease by blocking CD147-CypA interaction, then decreasing chemotaxis for the immunocyte, and reducing migration of immunocytes to the lung and collagen deposition in the lung.

Perceptions of Business Students' Feature Requirements in Educational Web Sites

ERIC Educational Resources Information Center

Hazari, Sunil; Johnson, Barbara

2007-01-01

There is paucity of original research that explains phenomena related to content organization and site design of educational Web sites. Educational Web sites are often used to provide Web-based instruction, which itself is a relatively recent phenomenon for business schools, and additional research is needed in this area. Educational Web sites are…

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... the General Mining Law, but not from the Mineral Leasing Act of 1920 (30 U.S.C. 181 et seq.) or the... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application...

43 CFR 2804.25 - How will BLM process my application?

Code of Federal Regulations, 2011 CFR

2011-10-01

... cultural resource surveys or inventories for threatened or endangered species. If BLM needs more... the General Mining Law, but not from the Mineral Leasing Act of 1920 (30 U.S.C. 181 et seq.) or the... FEDERAL LAND POLICY MANAGEMENT ACT Applying for FLPMA Grants § 2804.25 How will BLM process my application...

43 CFR 3602.14 - What kind of financial security does BLM require?

Code of Federal Regulations, 2011 CFR

2011-10-01

... DISPOSAL Mineral Materials Sales Applications § 3602.14 What kind of financial security does BLM require? (a) For contracts of $2,000 or more, BLM will require a performance bond of an amount sufficient to meet the reclamation standards provided for in the contract, but at least $500. If you have a sales...

75 FR 26986 - Notice of Intent To Prepare an Amendment to the Kobuk-Seward Peninsula Resource Management Plan...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-13

... Conservation Act of 1980 (ANILCA), and the BLM policies. The BLM will work collaboratively with interested...: The BLM-administered Squirrel River SRMA is located in western Alaska, approximately 30 miles... amendment and associated EA will meet the requirements of the National Environmental Policy Act of 1969...

43 CFR 2884.22 - Can BLM ask me for additional information?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Can BLM ask me for additional information? 2884.22 Section 2884.22 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU... THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.22 Can BLM ask me for additional...

43 CFR 2884.22 - Can BLM ask me for additional information?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Can BLM ask me for additional information? 2884.22 Section 2884.22 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU... THE MINERAL LEASING ACT Applying for MLA Grants or TUPs § 2884.22 Can BLM ask me for additional...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2013 CFR

2013-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2012 CFR

2012-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 3809.202 - Under what conditions will BLM defer to State regulation of operations?

Code of Federal Regulations, 2014 CFR

2014-10-01

... standards on a provision-by-provision basis to determine— (i) Whether non-numerical State standards are functionally equivalent to BLM counterparts; and (ii) Whether numerical State standards are the same as corresponding numerical BLM standards, except that State review and approval time frames do not have to be the...

43 CFR 2806.43 - How does BLM calculate rent for passive reflectors and local exchange networks?

Code of Federal Regulations, 2011 CFR

2011-10-01

... reflectors and local exchange networks? 2806.43 Section 2806.43 Public Lands: Interior Regulations Relating...-Of-Way § 2806.43 How does BLM calculate rent for passive reflectors and local exchange networks? (a) BLM calculates rent for passive reflectors and local exchange networks by using the same rent...

43 CFR 3602.27 - When will BLM extend the term of a contract?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false When will BLM extend the term of a contract? 3602.27 Section 3602.27 Public Lands: Interior Regulations Relating to Public Lands (Continued... DISPOSAL Mineral Materials Sales Administration of Sales § 3602.27 When will BLM extend the term of a...

43 CFR 3505.62 - Under what conditions will BLM extend my prospecting permit?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Under what conditions will BLM extend my prospecting permit? 3505.62 Section 3505.62 Public Lands: Interior Regulations Relating to Public Lands....62 Under what conditions will BLM extend my prospecting permit? You must prove that: (a) You explored...

43 CFR 3602.27 - When will BLM extend the term of a contract?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When will BLM extend the term of a contract? 3602.27 Section 3602.27 Public Lands: Interior Regulations Relating to Public Lands (Continued... DISPOSAL Mineral Materials Sales Administration of Sales § 3602.27 When will BLM extend the term of a...

43 CFR 3505.62 - Under what conditions will BLM extend my prospecting permit?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Under what conditions will BLM extend my prospecting permit? 3505.62 Section 3505.62 Public Lands: Interior Regulations Relating to Public Lands....62 Under what conditions will BLM extend my prospecting permit? You must prove that: (a) You explored...

76 FR 5201 - Notice of Realty Action: Competitive Sale of Public Lands in Monterey County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-28

... sale should be sent to the Field Manager, BLM Hollister Field Office, 20 Hamilton Court, Hollister, CA... will be provided an opportunity to submit supplemental bids. The BLM Hollister Field Office Manager... Management (BLM), Hollister Field Office, proposes to sell two separate parcels of public land totaling 80...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2012 CFR

2012-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2013 CFR

2013-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2011 CFR

2011-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 2884.11 - What information must I submit in my application?

Code of Federal Regulations, 2014 CFR

2014-10-01

... application? (a) File your application on Form SF-299 or as part of an Application for Permit to Drill or Reenter (BLM Form 3160-3) or Sundry Notice and Report on Wells (BLM Form 3160-5), available from any BLM... agencies, such as the Federal Energy Regulatory Commission (see 18 CFR chapter I), for licenses...

43 CFR 3264.11 - What must I submit to BLM after I finish subsequent well operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... subsequent well operations? 3264.11 Section 3264.11 Public Lands: Interior Regulations Relating to Public...) GEOTHERMAL RESOURCE LEASING Reports-Drilling Operations § 3264.11 What must I submit to BLM after I finish subsequent well operations? (a) Submit to BLM a subsequent well operations report within 30 days after...

78 FR 29379 - BLM Director's Response to the Appeal by the Governors of Utah and Wyoming of the BLM Assistant...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-20

... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLWO320000 L13100000 DT0000 LXSIOSHL0000... Director's Governor's Consistency Review Determination AGENCY: Bureau of Land Management, Interior. ACTION... Shale and Tar Sands Resources on Lands Administered by the Bureau of Land Management (BLM) in Colorado...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2013 CFR

2013-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2012 CFR

2012-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2014 CFR

2014-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

43 CFR 3931.100 - Boundary pillars and buffer zones.

Code of Federal Regulations, 2011 CFR

2011-10-01

... otherwise specified in writing by the BLM. Boundary and other main pillars may be mined only with the BLM's prior written consent or on the BLM's order. For in-situ operations, a 50-foot buffer zone from the..., mine out and remove all available oil shale in such boundary pillar, both in the lands covered by the...

Web site development: applying aesthetics to promote breast health education and awareness.

PubMed

Thomas, Barbara; Goldsmith, Susan B; Forrest, Anne; Marshall, Renée

2002-01-01

This article describes the process of establishing a Web site as part of a collaborative project using visual art to promote breast health education. The need for a more "user-friendly" comprehensive breast health Web site that is aesthetically rewarding was identified after an analysis of current Web sites available through the World Wide Web. Two predetermined sets of criteria, accountability and aesthetics, were used to analyze these sites and to generate ideas for creating a breast health education Web site using visual art. Results of the analyses conducted are included as well as the factors to consider for incorporating into a Web site. The process specified is thorough and can be applied to establish a Web site that is aesthetically rewarding and informative for a variety of educational purposes.

Changes in expression of cytokines in polyhexamethylene guanidine-induced lung fibrosis in mice: Comparison of bleomycin-induced lung fibrosis.

PubMed

Kim, Min-Seok; Kim, Sung-Hwan; Jeon, Doin; Kim, Hyeon-Young; Lee, Kyuhong

2018-01-15

Inhalation of polyhexamethylene guanidine (PHMG) causes irreversible pulmonary injury, such as pulmonary fibrosis. However, the mechanism underlying PHMG-induced lung injury is unclear. In this study, we compared the difference in time-dependent lung injury between PHMG- and bleomycin (BLM)-treated mice and determined cytokines involved in inducing lung injury by performing cytokine antibody array analysis. Mice were treated once with 1.8mg/kg BLM or 1.2mg/kg PHMG through intratracheal instillation and were sacrificed on days 7 and 28. Bronchoalveolar lavage fluid (BALF) analysis showed that the number of neutrophils was significantly higher in PHMG-treated mice than in BLM-treated mice on day 7. Histopathological analysis showed inflammatory cell infiltration and fibrosis mainly in the terminal bronchioles and alveoli in the lungs of PHMG- and BLM-treated mice. However, continuous macrophage infiltration in the alveolar space and bronchioloalveolar epithelial hyperplasia (BEH) were only observed in PHMG-treated mice. Cytokine antibody array analysis showed that 15 and eight cytokines were upregulated in PHMG- and BLM-treated mice, respectively, on day 7. On day 28, 13 and five cytokines were upregulated in PHMG and BLM-treated mice, respectively. In addition, the expressed cytokines between days 7 and 28 in BLM-treated mice were clearly different, but were similar in PHMG-treated mice. Consequently, between PHMG- and BLM-treated mice, we observed differences in the expression patterns and types of cytokines. These differences are considered to be a result of the inflammatory processes induced by both substances, which may mainly involve macrophage infiltration. Therefore, continuous induction of the inflammatory response by PHMG may play an important role in the development of pulmonary fibrosis. Copyright © 2017 Elsevier B.V. All rights reserved.

Photosynthesis and the web: 2001.

PubMed

Orr, L

2001-01-01

First, a brief history of the Internet and the World Wide Web is presented. This is followed by relevant information on photosynthesis-related web sites grouped into several categories: (1) large group sites, (2) comprehensive overview sites, (3) specific subject sites, (4) individual researcher sites, (5) kindergarten through high school (K-12) educational sites, (6) books and journals, and, 7) other useful sites. A section on searching the Web is also included. Finally, we have included an appendix with all of the web sites discussed herein as well as other web sites that space did not allow. Readers are requested to send comments, corrections and additions to gov@uiuc.edu.

How Japanese students characterize information from web-sites.

PubMed

Iwahara, A; Yamada, M; Hatta, T; Kawakami, A; Okamoto, M

2000-12-01

How 352 Japanese university students regard web-site information was investigated by two kinds of survey. Application of correspondence analysis and cluster analysis to the questionnaire responses to the web-site advertisement showed students regarded a web-site as a new alien medium which is different from current media. Students regarded web-sites as simply complicated, intellectual, and impermanent, or not memorable. Students got precise information from web-sites but they did not use it in making decisions to purchase goods.

Web sites for postpartum depression: convenient, frustrating, incomplete, and misleading.

PubMed

Summers, Audra L; Logsdon, M Cynthia

2005-01-01

To evaluate the content and the technology of Web sites providing information on postpartum depression. Eleven search engines were queried using the words "Postpartum Depression." The top 10 sites in each search engine were evaluated for correct content and technology using the Web Depression Tool, based on the Technology Assessment Model. Of the 36 unique Web sites located, 34 were available to review. Only five Web sites provided >75% correct responses to questions that summarized the current state of the science for postpartum depression. Eleven of the Web sites contained little or no useful information about postpartum depression, despite being among the first 10 Web sites listed by the search engine. Some Web sites contained possibly harmful suggestions for treatment of postpartum depression. In addition, there are many problems with the technology of Web sites providing information on postpartum depression. A better Web site for postpartum depression is necessary if we are to meet the needs of consumers for accurate and current information using technology that enhances learning. Since patient education is a core competency for nurses, it is essential that nurses understand how their patients are using the World Wide Web for learning and how we can assist our patients to find appropriate sites containing correct information.

16 CFR 1130.8 - Requirements for Web site registration or alternative e-mail registration.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for Web site registration or... PRODUCTS (Eff. June 28, 2010) § 1130.8 Requirements for Web site registration or alternative e-mail registration. (a) Link to registration page. The manufacturer's Web site, or other Web site established for the...

22 CFR 502.6 - Terms of use for accessing program materials available on agency Web sites.

Code of Federal Regulations, 2014 CFR

2014-04-01

... available on agency Web sites. 502.6 Section 502.6 Foreign Relations BROADCASTING BOARD OF GOVERNORS... program materials available on agency Web sites. (a) By accessing Agency Web sites, Requestors agree to all the Terms of Use available on those Web sites. (b) All Requestors are advised that Agency program...

16 CFR 1130.8 - Requirements for Web site registration or alternative e-mail registration.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Requirements for Web site registration or... PRODUCTS § 1130.8 Requirements for Web site registration or alternative e-mail registration. (a) Link to registration page. The manufacturer's Web site, or other Web site established for the purpose of registration...

16 CFR 1130.8 - Requirements for Web site registration or alternative e-mail registration.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Requirements for Web site registration or... PRODUCTS § 1130.8 Requirements for Web site registration or alternative e-mail registration. (a) Link to registration page. The manufacturer's Web site, or other Web site established for the purpose of registration...

16 CFR 1130.7 - Requirements for Web site registration or alternative e-mail registration.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Requirements for Web site registration or... PRODUCTS § 1130.7 Requirements for Web site registration or alternative e-mail registration. (a) Link to registration page. The manufacturer's Web site, or other Web site established for the purpose of registration...

16 CFR § 1130.8 - Requirements for Web site registration or alternative e-mail registration.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Requirements for Web site registration or... OR TODDLER PRODUCTS § 1130.8 Requirements for Web site registration or alternative e-mail registration. (a) Link to registration page. The manufacturer's Web site, or other Web site established for the...

Characteristics of food industry web sites and "advergames" targeting children.

PubMed

Culp, Jennifer; Bell, Robert A; Cassady, Diana

2010-01-01

To assess the content of food industry Web sites targeting children by describing strategies used to prolong their visits and foster brand loyalty; and to document health-promoting messages on these Web sites. A content analysis was conducted of Web sites advertised on 2 children's networks, Cartoon Network and Nickelodeon. A total of 290 Web pages and 247 unique games on 19 Internet sites were examined. Games, found on 81% of Web sites, were the most predominant promotion strategy used. All games had at least 1 brand identifier, with logos being most frequently used. On average Web sites contained 1 "healthful" message for every 45 exposures to brand identifiers. Food companies use Web sites to extend their television advertising to promote brand loyalty among children. These sites almost exclusively promoted food items high in sugar and fat. Health professionals need to monitor food industry marketing practices used in "new media." Published by Elsevier Inc.

Facilitating Oil Industry Access to Federal Lands through Interagency Data Sharing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paul Jehn; Ben Grunewald

Much of the environmental and technical data useful to the oil and gas industry and regulatory agencies is now contained in disparate state and federal databases. Delays in coordinating permit approvals between federal and state agencies translate into increased operational costs and stresses for the oil and gas industry. Making federal lease stipulation and area restriction data available on state agency Web sites will streamline a potential lessors review of available leases, encourage more active bidding on unleased federal lands, and give third-party operators independent access to data who otherwise may not have access to lease restrictions and other environmentalmore » data. As a requirement of the Energy Policy Conservation Act (EPCA), the Bureau of Land Management (BLM) is in the process of inventorying oil and natural gas resources beneath onshore federal lands and the extent and nature of any stipulation, restrictions, or impediments to the development of these resources. The EPCA Phase 1 Inventory resulted in a collection of GIS coverage files organized according to numerous lease stipulation reference codes. Meanwhile, state agencies also collect millions of data elements concerning oil and gas operations. Much of the oil and gas data nationwide is catalogued in the Ground Water Protection Council's (GWPC's) successfully completed Risk Based Data Management System (RBDMS). The GWPC and the states of Colorado, New Mexico, Utah, and Montana are implementing a pilot project where BLM lease stipulation data and RBDMS data will be displayed in a GIS format on the Internet. This increased access to data will increase bid activity, help expedite permitting, and encourage exploration on federal lands. Linking environmental, lease stipulation and resource inventory assessment data and making a GIS interface for the data available to industry and other agencies via the internet represents an important step in the GWPC strategy for all oil and gas regulatory e-commerce. The next step beyond mere data sharing for facilitating the permitting process is to make it possible for industry to file those permit applications electronically. This process will involve the use of common XML schemas.« less

Biotic ligand modeling approach: Synthesis of the effect of major cations on the toxicity of metals to soil and aquatic organisms.

PubMed

Ardestani, Masoud M; van Straalen, Nico M; van Gestel, Cornelis A M

2015-10-01

The biotic ligand model (BLM) approach is used to assess metal toxicity, taking into account the competition of other cations with the free metal ions for binding to the biotic ligand sites of aquatic and soil organisms. The bioavailable fraction of metals, represented by the free metal ion, is a better measure than the total concentration for assessing their potential risk to the environment. Because BLMs are relating toxicity to the fraction of biotic ligands occupied by the metal, they can be useful for investigating factors affecting metal bioaccumulation and toxicity. In the present review, the effects of major cations on the toxicity of metals to soil and aquatic organisms were comprehensively studied by performing a meta-analysis of BLM literature data. Interactions at the binding sites were shown to be species- and metal-specific. The main factors affecting the relationships between toxicity and conditional binding constants for metal binding at the biotic ligand appeared to be Ca(2+) , Mg(2+) , and protons. Other important characteristics of the exposure medium, such as levels of dissolved organic carbon and concentrations of other cations, should also be considered to obtain a proper assessment of metal toxicity to soil and aquatic organisms. © 2015 SETAC.

Relative contribution of homologous recombination and non-homologous end-joining to DNA double-strand break repair after oxidative stress in Saccharomyces cerevisiae.

PubMed

Letavayová, Lucia; Marková, Eva; Hermanská, Katarína; Vlcková, Viera; Vlasáková, Danusa; Chovanec, Miroslav; Brozmanová, Jela

2006-05-10

Oxidative damage to DNA seems to be an important factor in developing many human diseases including cancer. It involves base and sugar damage, base-free sites, DNA-protein cross-links and DNA single-strand (SSB) and double-strand (DSB) breaks. Oxidative DSB can be formed in various ways such as their direct induction by the drug or their generation either through attempted and aborted repair of primary DNA lesions or through DNA replication-dependent conversion of SSB. In general, two main pathways are responsible for repairing DSB, homologous recombination (HR) and non-homologous end-joining (NHEJ), with both of them being potential candidates for the repair of oxidative DSB. We have examined relative contribution of HR and NHEJ to cellular response after oxidative stress in Saccharomyces cerevisiae. Therefore, cell survival, mutagenesis and DSB induction and repair in the rad52, yku70 and rad52 yku70 mutants after hydrogen peroxide (H(2)O(2)), menadione (MD) or bleomycin (BLM) exposure were compared to those obtained for the corresponding wild type. We show that MD exposure does not lead to observable DSB induction in yeast, suggesting that the toxic effects of this agent are mediated by other types of DNA damage. Although H(2)O(2) treatment generates some DSB, their yield is relatively low and hence DSB may only partially be responsible for toxicity of H(2)O(2), particularly at high doses of the agent. On the other hand, the basis of the BLM toxicity resides primarily in DSB induction. Both HR and NHEJ act on BLM-induced DSB, although their relative participation in the process is not equal. Based on our results we suggest that the complexity and/or the quality of the BLM-induced DSB might represent an obstacle for the NHEJ pathway.

Copper toxicity and organic matter: Resiliency of watersheds in the Duluth Complex, Minnesota, USA

USGS Publications Warehouse

Piatak, Nadine; Seal, Robert; Jones, Perry M.; Woodruff, Laurel G.

2015-01-01

We estimated copper (Cu) toxicity in surface water with high dissolved organic matter (DOM) for unmined mineralized watersheds of the Duluth Complex using the Biotic Ligand Model (BLM), which evaluates the effect of DOM, cation competition for biologic binding sites, and metal speciation. A sediment-based BLM was used to estimate stream-sediment toxicity; this approach factors in the cumulative effects of multiple metals, incorporation of metals into less bioavailable sulfides, and complexation of metals with organic carbon. For surface water, the formation of Cu-DOM complexes significantly reduces the amount of Cu available to aquatic organisms. The protective effects of cations, such as calcium (Ca) and magnesium (Mg), competing with Cu to complex with the biotic ligand is likely not as important as DOM in water with high DOM and low hardness. Standard hardness-based water quality criteria (WQC) are probably inadequate for describing Cu toxicity in such waters and a BLM approach may yield more accurate results. Nevertheless, assumptions about relative proportions of humic acid (HA) and fulvic acid (FA) in DOM significantly influence BLM results; the higher the HA fraction, the higher calculated resiliency of the water to Cu toxicity. Another important factor is seasonal variation in water chemistry, with greater resiliency to Cu toxicity during low flow compared to high flow.Based on generally low total organic carbon and sulfur content, and equivalent metal ratios from total and weak partial extractions, much of the total metal concentration in clastic streambedsediments may be in bioavailable forms, sorbed on clays or hydroxide phases. However, organicrich fine-grained sediment in the numerous wetlands may sequester significant amount of metals, limiting their bioavailability. A high proportion of organic matter in waters and some sediments will play a key role in the resiliency of these watersheds to potential additional metal loads associated with future mining operations.

77 FR 71822 - Notice of Availability of the BLM's Responses to Public Comments and of the BLM's Environmental...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-04

... century. In 1925, potash (potassium-bearing salts primarily used for fertilizer) was discovered in this... is found in formations below the potash-bearing formations, so oil and gas wells must extend through... industries. This conflict has resulted in a great deal of litigation regarding decisions made by the BLM on a...

43 CFR 3508.14 - How will BLM publish the notice of lease sale?

Code of Federal Regulations, 2011 CFR

2011-10-01

... notice of lease sale at least once a week for three consecutive weeks in a newspaper of general circulation in the area where the lands are situated. We will also post the notice of lease sale for 30 days...; (3) A description of the tract BLM is offering; (4) A description of the mineral deposit BLM is...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2012 CFR

2012-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2014 CFR

2014-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2011 CFR

2011-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

43 CFR 2808.11 - What will BLM do if it determines that I am in trespass?

Code of Federal Regulations, 2013 CFR

2013-10-01

... am in trespass? 2808.11 Section 2808.11 Public Lands: Interior Regulations Relating to Public Lands...-WAY UNDER THE FEDERAL LAND POLICY MANAGEMENT ACT Trespass § 2808.11 What will BLM do if it determines that I am in trespass? (a) BLM will notify you in writing of the trespass and explain your liability...

78 FR 8188 - Notice of Realty Action: Proposed (Non-Competitive) Direct Sale of Public Land in Carbon County, UT

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-05

... by March 22, 2013. ADDRESSES: Address all written comments concerning this notice to the BLM, Price Field Office, Attn: Connie Leschin, 125 S. 600 W., Price, Utah, 84501. FOR FURTHER INFORMATION CONTACT... survey of the said land, on file with the BLM. The proposed sale is in conformance with the BLM Price...

A GIS-based method to evaluate undeveloped BLM lands in Alaska

Treesearch

Jason Geck

2007-01-01

As Alaska’s largest land management agency, the Bureau of Land Management (BLM) has responsibility for over 87 million acres (35 million ha) of public lands throughout the state. By using datasets and Landsat scenes within a Geographical Information System (GIS), this study prioritizes wilderness protection through the ranking of BLM blocks (contiguous land parcels),...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2012 CFR

2012-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2013 CFR

2013-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2011 CFR

2011-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 3280.5 - May BLM require the modification of lease requirements in connection with the creation and...

Code of Federal Regulations, 2014 CFR

2014-10-01

... requirements in connection with the creation and operation of a unit agreement? 3280.5 Section 3280.5 Public... with the creation and operation of a unit agreement? (a) BLM may, with the consent of the lessees... connection with the creation and operation of any such unit agreement as BLM may consider necessary or...

43 CFR 2806.15 - Under what circumstances may BLM waive or reduce my rent?

Code of Federal Regulations, 2011 CFR

2011-10-01

... qualifies for a waiver or a reduction of rent. (b) BLM may waive or reduce your rent if you show BLM that: (1) You are a non-profit organization, corporation, or association which is not controlled by, or is not a subsidiary of, a profit making corporation or business enterprise and the facility or project...

Bleomycin enhances the efficacy of sonodynamic therapy using aluminum phthalocyanine disulfonate.

PubMed

Osaki, Tomohiro; Yokoe, Inoru; Uto, Yoshihiro; Ishizuka, Masahiro; Tanaka, Toru; Yamanaka, Nobuyasu; Kurahashi, Tsukasa; Azuma, Kazuo; Murahata, Yusuke; Tsuka, Takeshi; Ito, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu

2016-01-01

Sonodynamic therapy (SDT), or ultrasound combined with sonosensitization, is a promising approach because it is noninvasive and penetrates deeper than light does in photodynamic therapy. We examined whether bleomycin (BLM) could improve the efficacy of SDT. We performed an in vitro study using Colon-26 cells, which are derived from mouse colon cancer. SDT with BLM was significantly more cytotoxic than SDT alone both in vitro and in vivo. We also observed an ultrasound intensity-dependent cytotoxic effect of SDT with BLM. These findings suggest that SDT with BLM might provide a novel noninvasive treatment for deep-seated tumors. Copyright © 2015 Elsevier B.V. All rights reserved.

Strain improvement by combined UV mutagenesis and ribosome engineering and subsequent fermentation optimization for enhanced 6'-deoxy-bleomycin Z production.

PubMed

Zhu, Xiangcheng; Kong, Jieqian; Yang, Hu; Huang, Rong; Huang, Yong; Yang, Dong; Shen, Ben; Duan, Yanwen

2018-02-01

The bleomycins (BLMs) are important clinical drugs extensively used in combination chemotherapy for the treatment of various cancers. Dose-dependent lung toxicity and the development of drug resistance have restricted their wide applications. 6'-Deoxy-BLM Z, a recently engineered BLM analogue with improved antitumor activity, has the potential to be developed into the next-generation BLM anticancer drug. However, its low titer in the recombinant strain Streptomyces flavoviridis SB9026 has hampered current efforts, which require sufficient compound, to pursue preclinical studies and subsequent clinical development. Here, we report the strain improvement by combined UV mutagenesis and ribosome engineering, as well as the fermentation optimization, for enhanced 6'-deoxy-BLM production. A high producer, named S. flavoviridis G-4F12, was successfully isolated, producing 6'-deoxy-BLM at above 70 mg/L under the optimized fermentation conditions, representing a sevenfold increase in comparison with that of the original producer. These findings demonstrated the effectiveness of combined empirical breeding methods in strain improvement and set the stage for sustainable production of 6'-deoxy-BLM via pilot-scale microbial fermentation.

Crystal Structures of RMI1 and RMI2, Two OB-Fold Regulatory Subunits of the BLM Complex

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Feng; Yang, Yuting; Singh, Thiyam Ramsing

Mutations in BLM, a RecQ-like helicase, are linked to the autosomal recessive cancer-prone disorder Bloom's syndrome. BLM associates with topoisomerase (Topo) III{alpha}, RMI1, and RMI2 to form the BLM complex that is essential for genome stability. The RMI1-RMI2 heterodimer stimulates the dissolution of double Holliday junction into non-crossover recombinants mediated by BLM-Topo III{alpha} and is essential for stabilizing the BLM complex. However, the molecular basis of these functions of RMI1 and RMI2 remains unclear. Here we report the crystal structures of multiple domains of RMI1-RMI2, providing direct confirmation of the existence of three oligonucleotide/oligosaccharide binding (OB)-folds in RMI1-RMI2. Our structuralmore » and biochemical analyses revealed an unexpected insertion motif in RMI1N-OB, which is important for stimulating the dHJ dissolution. We also revealed the structural basis of the interaction between RMI1C-OB and RMI2-OB and demonstrated the functional importance of the RMI1-RMI2 interaction in genome stability maintenance.« less

Histopathological Correlations between Mediastinal Fat-Associated Lymphoid Clusters and the Development of Lung Inflammation and Fibrosis following Bleomycin Administration in Mice.

PubMed

Elewa, Yaser Hosny Ali; Ichii, Osamu; Takada, Kensuke; Nakamura, Teppei; Masum, Md Abdul; Kon, Yasuhiro

2018-01-01

Bleomycin (BLM) has been reported to induce lung inflammation and fibrosis in human and mice and showed genetic susceptibility. Interestingly, the C57BL/6 (B6) mice had prominent mediastinal fat-associated lymphoid cluster (MFALCs) under healthy condition, and showed susceptibility to development of lung fibrosis following BLM administration. However, the pathogenesis of lung lesion progression, and their correlation with MFALC morphologies, remain to be clarified. To investigate the correlations between MFALC structures and lung injuries in B6 mice, histopathological examination of mediastinal fat tissues and lungs was examined at 7 and 21 days (d) following a single 50 μL intranasal (i.n.) instillation of either BLM sulfate (5 mg/kg) (BLM group) or phosphate-buffered saline (control group). The lung fibrosis was examined by Masson's trichrome (MT) stain of paraffin sections and mRNA expression levels of Col1a1, Col3a1, and Acta2 in different frozen lung samples. Furthermore, immunohistochemistry for CD3, B220, Iba1, Gr1, BrdU, LYVE-1, and peripheral node addressin (PNAd) was performed to detect T- and B-cells, macrophages, granulocytes, proliferating cells, lymph vessels (LVs), and high endothelial venules (HEVs). We found that MFALCs were more abundant in the BLM group as compared to the control group. The lung of BLM group developed pneumonitis with severe cellular infiltrations at 7 days and significant collagen deposition (MT) and higher expression of Col1a1, and Col3a1 at 21 days post-administration. Numerous immune cells, proliferating cells, HEVs, and LVs were observed in both MFALCs and lungs of the BLM group. Interestingly, PNAd + HEVs were observed in the lungs of the BLM group, but not the control group. Moreover, numerous Gr1 + polymorphonuclear and mononuclear-like ring cells were found in the MFALCs and lungs of the BLM group. Interestingly, flow cytometric analysis revealed a significant increase of B-cell populations within the MFALCs of BLM group suggesting a potential proliferative induction of B-cells following inflammation. Furthermore, significant positive correlations were observed between quantitative parameters of these immune cells in both the lungs and MFALCs. Thus, we suggest a potentially important role for MFALCs and HEVs in the progression of lung disease, especially in inflammatory lung disease.

The effect of types of banner ad, Web localization, and customer involvement on Internet users' attitudes.

PubMed

Chen, Jengchung Victor; Ross, William H; Yen, David C; Akhapon, Lerdsuwankij

2009-02-01

In this study, three characteristics of Web sites were varied: types of banner ad, Web localization, and involvement in purchasing a product. The dependent variable was attitude toward the site. In laboratory experiments conducted in Thailand and Taiwan, participants browsed versions of a Web site containing different types of banner ads and products. As a within-participants factor, each participant browsed both a standardized English-language Web site and a localized Web site. Results showed that animated (rather than static) banner ads, localized versions (rather than a standardized version) of Web sites, and high (rather than low) product involvement led to favorable attitudes toward the site.

ACHP | Web Site Privacy Policy

Science.gov Websites

Search skip specific nav links Home arrow About ACHP arrow Web Site Privacy Policy ACHP Web Site Privacy be used after its purpose has been fulfilled. For questions on our Web site privacy policy, please contact the Web manager. Updated October 2, 2006 Return to Top

A Two-Tiered Model for Analyzing Library Web Site Usage Statistics, Part 1: Web Server Logs.

ERIC Educational Resources Information Center

Cohen, Laura B.

2003-01-01

Proposes a two-tiered model for analyzing web site usage statistics for academic libraries: one tier for library administrators that analyzes measures indicating library use, and a second tier for web site managers that analyzes measures aiding in server maintenance and site design. Discusses the technology of web site usage statistics, and…

Analysis of governmental Web sites on food safety issues: a global perspective.

PubMed

Namkung, Young; Almanza, Barbara A

2006-10-01

Despite a growing concern over food safety issues, as well as a growing dependence on the Internet as a source of information, little research has been done to examine the presence and relevance of food safety-related information on Web sites. The study reported here conducted Web site analysis in order to examine the current operational status of governmental Web sites on food safety issues. The study also evaluated Web site usability, especially information dimensionalities such as utility, currency, and relevance of content, from the perspective of the English-speaking consumer. Results showed that out of 192 World Health Organization members, 111 countries operated governmental Web sites that provide information about food safety issues. Among 171 searchable Web sites from the 111 countries, 123 Web sites (71.9 percent) were accessible, and 81 of those 123 (65.9 percent) were available in English. The majority of Web sites offered search engine tools and related links for more information, but their availability and utility was limited. In terms of content, 69.9 percent of Web sites offered information on foodborne-disease outbreaks, compared with 31.5 percent that had travel- and health-related information.

Focused ultrasound-mediated sonochemical internalization: an alternative to light-based therapies

NASA Astrophysics Data System (ADS)

Gonzales, Jonathan; Nair, Rohit Kumar; Madsen, Steen J.; Krasieva, Tatiana; Hirschberg, Henry

2016-07-01

Activation of sonosensitizers via focused ultrasound (FUS), i.e., sonodynamic therapy has been proposed as an extension to light-activated photodynamic therapy for the treatment of brain as well as other tumors. The use of FUS, as opposed to light, allows treatment to tumor sites buried deep within tissues as well as through the intact skull. We have examined ultrasonic activation of sonosensitizers together with the anticancer agent bleomycin (BLM), i.e., sonochemical internalization (SCI). SCI is a technique that utilizes FUS for the enhanced delivery of endo-lysosomal trapped macromolecules into the cell cytoplasm in a similar manner to light-based photochemical internalization. The released agent can, therefore, exert its full biological activity, in contrast to being degraded by lysosomal hydrolases. Our results indicate that, compared to drug or FUS treatment alone, FUS activation of the sonosensitizer AlPcS2a together with BLM significantly inhibits the ability of treated glioma cells to grow as three-dimensional tumor spheroids in vitro.

ACHP | Other Historic Preservation Web Sites of Interest

Science.gov Websites

Preservation Web Sites of Interest Other Historic Preservation Web Sites of Interest National Transportation an organization's link on the ACHP's Web site does not imply endorsement of the organization or its

Evaluating Domestic and International Web-Site Strategies.

ERIC Educational Resources Information Center

Simeon, Roblyn

1999-01-01

Presents the AIPD (attracting, informing, positioning, and delivering) approach to the evaluation of commercial Web sites that assess the strategic potential of Web sites, provides a framework for the development of competitive sites, and compares Web site strategies within and across national boundaries. Compares Internet strategies of Japanese…

77 FR 74266 - Review of National Environmental Policy Act Categorical Exclusion Survey Posted on DOT/FHWA Web Site

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-13

... of National Environmental Policy Act Categorical Exclusion Survey Posted on DOT/FHWA Web Site AGENCY... review is now available on the FHWA Web site, http://www.fhwa.dot.gov/map21 , and FTA Web site, http://www.fta.dot.gov/map21 . DATES: These reports were posted on the Web site on December 7, 2012...

10 CFR 905.23 - What are the opportunities for using the Freedom of Information Act to request plan and report data?

Code of Federal Regulations, 2010 CFR

2010-01-01

...'s publicly available Web site or on Western's Web site. Customers posting their IRPs on their own Web site must notify Western of this decision when they submit their IRP. A hotlink on Western's Web site to IRPs posted on customer Web sites gives interested parties ready access to those IRPs. Western...

10 CFR 905.23 - What are the opportunities for using the Freedom of Information Act to request plan and report data?

Code of Federal Regulations, 2014 CFR

2014-01-01

...'s publicly available Web site or on Western's Web site. Customers posting their IRPs on their own Web site must notify Western of this decision when they submit their IRP. A hotlink on Western's Web site to IRPs posted on customer Web sites gives interested parties ready access to those IRPs. Western...

10 CFR 905.23 - What are the opportunities for using the Freedom of Information Act to request plan and report data?

Code of Federal Regulations, 2011 CFR

2011-01-01

...'s publicly available Web site or on Western's Web site. Customers posting their IRPs on their own Web site must notify Western of this decision when they submit their IRP. A hotlink on Western's Web site to IRPs posted on customer Web sites gives interested parties ready access to those IRPs. Western...

10 CFR 905.23 - What are the opportunities for using the Freedom of Information Act to request plan and report data?

Code of Federal Regulations, 2012 CFR

2012-01-01

...'s publicly available Web site or on Western's Web site. Customers posting their IRPs on their own Web site must notify Western of this decision when they submit their IRP. A hotlink on Western's Web site to IRPs posted on customer Web sites gives interested parties ready access to those IRPs. Western...

10 CFR 905.23 - What are the opportunities for using the Freedom of Information Act to request plan and report data?

Code of Federal Regulations, 2013 CFR

2013-01-01

...'s publicly available Web site or on Western's Web site. Customers posting their IRPs on their own Web site must notify Western of this decision when they submit their IRP. A hotlink on Western's Web site to IRPs posted on customer Web sites gives interested parties ready access to those IRPs. Western...

Evaluation of the content and accessibility of web sites for accredited orthopaedic sports medicine fellowships.

PubMed

Mulcahey, Mary K; Gosselin, Michelle M; Fadale, Paul D

2013-06-19

The Internet is a common source of information for orthopaedic residents applying for sports medicine fellowships, with the web sites of the American Orthopaedic Society for Sports Medicine (AOSSM) and the San Francisco Match serving as central databases. We sought to evaluate the web sites for accredited orthopaedic sports medicine fellowships with regard to content and accessibility. We reviewed the existing web sites of the ninety-five accredited orthopaedic sports medicine fellowships included in the AOSSM and San Francisco Match databases from February to March 2012. A Google search was performed to determine the overall accessibility of program web sites and to supplement information obtained from the AOSSM and San Francisco Match web sites. The study sample consisted of the eighty-seven programs whose web sites connected to information about the fellowship. Each web site was evaluated for its informational value. Of the ninety-five programs, fifty-one (54%) had links listed in the AOSSM database. Three (3%) of all accredited programs had web sites that were linked directly to information about the fellowship. Eighty-eight (93%) had links listed in the San Francisco Match database; however, only five (5%) had links that connected directly to information about the fellowship. Of the eighty-seven programs analyzed in our study, all eighty-seven web sites (100%) provided a description of the program and seventy-six web sites (87%) included information about the application process. Twenty-one web sites (24%) included a list of current fellows. Fifty-six web sites (64%) described the didactic instruction, seventy (80%) described team coverage responsibilities, forty-seven (54%) included a description of cases routinely performed by fellows, forty-one (47%) described the role of the fellow in seeing patients in the office, eleven (13%) included call responsibilities, and seventeen (20%) described a rotation schedule. Two Google searches identified direct links for 67% to 71% of all accredited programs. Most accredited orthopaedic sports medicine fellowships lack easily accessible or complete web sites in the AOSSM or San Francisco Match databases. Improvement in the accessibility and quality of information on orthopaedic sports medicine fellowship web sites would facilitate the ability of applicants to obtain useful information.

Assessing the concentration, speciation, and toxicity of dissolved metals during mixing of acid-mine drainage and ambient river water downstream of the Elizabeth Copper Mine, Vermont, USA

USGS Publications Warehouse

Balistrieri, L.S.; Seal, R.R.; Piatak, N.M.; Paul, B.

2007-01-01

The authors determine the composition of a river that is impacted by acid-mine drainage, evaluate dominant physical and geochemical processes controlling the composition, and assess dissolved metal speciation and toxicity using a combination of laboratory, field and modeling studies. Values of pH increase from 3.3 to 7.6 and the sum of dissolved base metal (Cd + Co + Cu + Ni + Pb + Zn) concentrations decreases from 6270 to 100 ??g/L in the dynamic mixing and reaction zone that is downstream of the river's confluence with acid-mine drainage. Mixing diagrams and PHREEQC calculations indicate that mixing and dilution affect the concentrations of all dissolved elements in the reach, and are the dominant processes controlling dissolved Ca, K, Li, Mn and SO4 concentrations. Additionally, dissolved Al and Fe concentrations decrease due to mineral precipitation (gibbsite, schwertmannite and ferrihydrite), whereas dissolved concentrations of Cd, Co, Cu, Ni, Pb and Zn decrease due to adsorption onto newly formed Fe precipitates. The uptake of dissolved metals by aquatic organisms is dependent on the aqueous speciation of the metals and kinetics of complexation reactions between metals, ligands and solid surfaces. Dissolved speciation of Cd, Cu, Ni and Zn in the mixing and reaction zone is assessed using the diffusive gradients in thin films (DGT) technique and results of speciation calculations using the Biotic Ligand Model (BLM). Data from open and restricted pore DGT units indicate that almost all dissolved metal species are inorganic and that aqueous labile or DGT available metal concentrations are generally equal to total dissolved concentrations in the mixing zone. Exceptions occur when labile metal concentrations are underestimated due to competition between H+ and metal ions for Chelex-100 binding sites in the DGT units at low pH values. Calculations using the BLM indicate that dissolved Cd and Zn species in the mixing and reaction zone are predominantly inorganic, which is consistent with the DGT results. Although the DGT method indicates that the majority of aqueous Cu species are inorganic, BLM calculations indicate that dissolved Cu is inorganic at pH 5.5. Integrated dissolved labile concentrations of Cd, Cu and Zn in the mixing and reaction zone are compared to calculated acute toxicity concentrations (LC50 values) for fathead minnows (Pimephales promelas) (Cd, Cu and Zn) and water fleas (Ceriodaphnia dubia) (Cd and Cu) using the BLM, and to national recommended water quality criteria [i.e., criteria maximum concentration (CMC) and criterion continuous concentration (CCC)]. Observed labile concentrations of Cd and Zn are below LC50 values and CMC for Cd, but above CCC and CMC for Zn at sites <30 m downstream of the confluence. In contrast, labile Cu concentrations exceed LC50 values for the organisms as well as CCC and CMC at sites <30 m downstream of the confluence. These results suggest that environmental conditions at sites closest to the confluence of the river and acid-mine drainage should not support healthy aquatic organisms. ?? 2007 Elsevier Ltd. All rights reserved.

30 CFR 218.305 - How do I pay advanced royalties I owe under BLM regulations?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 2 2010-07-01 2010-07-01 false How do I pay advanced royalties I owe under BLM... Geothermal Resources § 218.305 How do I pay advanced royalties I owe under BLM regulations? If you pay advanced royalties under 43 CFR 3212.15(a)(1) to retain your lease: (a) You must pay an advanced royalty...

30 CFR 1218.305 - How do I pay advanced royalties I owe under BLM regulations?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 3 2011-07-01 2011-07-01 false How do I pay advanced royalties I owe under BLM... CREDITS AND INCENTIVES Geothermal Resources § 1218.305 How do I pay advanced royalties I owe under BLM regulations? If you pay advanced royalties under 43 CFR 3212.15(a)(1) to retain your lease: (a) You must pay...

[Mechanism involving blm gene underlies repair of DNA damage of Jurkat cells induced by mitomycin C].

PubMed

Yi, Xue; Cheng, Hui; Zou, Ping; Liu, Ling-Bo; Zhang, Ting; Yu, Dan; Zhu, Xiao-Ming; Zou, Liang

2010-10-01

The defect or block of apoptosis is an important factor involved in the drug resistance of tumor cells. Blm gene plays a great role in DNA damage and repair. This study was aimed to explore the relationship of blm gene expression with cell cycle and apoptosis after Jurkat DNA damage. The apoptosis rate and change of cell cycle were detected by flow cytometry, the expression level of blm mRNA in Jurkat cells was determined by semi-quantitative RT-PCR. The results indicated that after induction with 0.4 g/L of mitomycin C (MMC) for 24 hours the apoptosis rate of Jurkat cells were (11.42±0.013)%, and (66.08±1.60)% Jurkat cells were arrested in G2/M phase. After induction for 48 hours, the apoptosis rate of Jurkat cells declined from (11.42±0.013)% to (8.08±0.27)%, and cell count of Jurkat cells arrested in G2/M phase decreased from (66.08±1.60)% to (33.96±1.05)%. When induced with 0.4 g/L of MMC for 24 hours, the apoptosis rate of fibroblasts and the percentage of fibroblasts in G2/M, G0-G1 and S phase all showed no significant change until 48 hours. The range of apoptosis rate and the change of cell percentage in three phases were significantly different between Jurkat cells and fibroblasts (p<0.01). Expression level of blm mRNA in Jurkat cells was remarkably higher than that in normal fibroblasts (p<0.01), at 48 hours expression level of blm mRNA was remarkably higher than that at 24 hours. The 2 groups showed clear difference of blm mRNA expression after treated by MMC (p<0.01). It is concluded that the blm gene may play a significant role in repair of DNA damage of Jurkat cells after MMC induction. Abnormal expression of blm is correlated to the drug resistance of leukemia cells.

Probabilistic approaches to accounting for data variability in the practical application of bioavailability in predicting aquatic risks from metals.

PubMed

Ciffroy, Philippe; Charlatchka, Rayna; Ferreira, Daniel; Marang, Laura

2013-07-01

The biotic ligand model (BLM) theoretically enables the derivation of environmental quality standards that are based on true bioavailable fractions of metals. Several physicochemical variables (especially pH, major cations, dissolved organic carbon, and dissolved metal concentrations) must, however, be assigned to run the BLM, but they are highly variable in time and space in natural systems. This article describes probabilistic approaches for integrating such variability during the derivation of risk indexes. To describe each variable using a probability density function (PDF), several methods were combined to 1) treat censored data (i.e., data below the limit of detection), 2) incorporate the uncertainty of the solid-to-liquid partitioning of metals, and 3) detect outliers. From a probabilistic perspective, 2 alternative approaches that are based on log-normal and Γ distributions were tested to estimate the probability of the predicted environmental concentration (PEC) exceeding the predicted non-effect concentration (PNEC), i.e., p(PEC/PNEC>1). The probabilistic approach was tested on 4 real-case studies based on Cu-related data collected from stations on the Loire and Moselle rivers. The approach described in this article is based on BLM tools that are freely available for end-users (i.e., the Bio-Met software) and on accessible statistical data treatments. This approach could be used by stakeholders who are involved in risk assessments of metals for improving site-specific studies. Copyright © 2013 SETAC.

Using Web Server Logs in Evaluating Instructional Web Sites.

ERIC Educational Resources Information Center

Ingram, Albert L.

2000-01-01

Web server logs contain a great deal of information about who uses a Web site and how they use it. This article discusses the analysis of Web logs for instructional Web sites; reviews the data stored in most Web server logs; demonstrates what further information can be gleaned from the logs; and discusses analyzing that information for the…

The Effectiveness of Course Web Sites in Higher Education: An Exploratory Study.

ERIC Educational Resources Information Center

Comunale, Christie L.; Sexton, Thomas R.; Voss, Diana J. Pedagano

2002-01-01

Describes an exploratory study of the educational effectiveness of course Web sites among undergraduate accounting students and graduate students in business statistics. Measured Web site visit frequency, usefulness of each site feature, and the impacts of Web sites on perceived learning and course performance. (Author/LRW)

Building a Library Web Site on the Pillars of Web 2.0

ERIC Educational Resources Information Center

Coombs, Karen A.

2007-01-01

In this article, the author discusses a project they undertook to reshape the libraries' Web services of the University of Houston site. The site had been in a state of flux and it needed a new structure for both managing and organizing it. She realized the staff was looking for a Web site that was more "Web 2.0" in nature. Web 2.0 is often…

A review of guidelines on home drug testing web sites for parents.

PubMed

Washio, Yukiko; Fairfax-Columbo, Jaymes; Ball, Emily; Cassey, Heather; Arria, Amelia M; Bresani, Elena; Curtis, Brenda L; Kirby, Kimberly C

2014-01-01

To update and extend prior work reviewing Web sites that discuss home drug testing for parents, and assess the quality of information that the Web sites provide, to assist them in deciding when and how to use home drug testing. We conducted a worldwide Web search that identified 8 Web sites providing information for parents on home drug testing. We assessed the information on the sites using a checklist developed with field experts in adolescent substance abuse and psychosocial interventions that focus on urine testing. None of the Web sites covered all the items on the 24-item checklist, and only 3 covered at least half of the items (12, 14, and 21 items, respectively). The remaining 5 Web sites covered less than half of the checklist items. The mean number of items covered by the Web sites was 11. Among the Web sites that we reviewed, few provided thorough information to parents regarding empirically supported strategies to effectively use drug testing to intervene on adolescent substance use. Furthermore, most Web sites did not provide thorough information regarding the risks and benefits to inform parents' decision to use home drug testing. Empirical evidence regarding efficacy, benefits, risks, and limitations of home drug testing is needed.

Information about Sexual Health on Crisis Pregnancy Center Web Sites: Accurate for Adolescents?

PubMed

Bryant-Comstock, Katelyn; Bryant, Amy G; Narasimhan, Subasri; Levi, Erika E

2016-02-01

The objective of this study was to evaluate the quality and accuracy of sexual health information on crisis pregnancy center Web sites listed in state resource directories for pregnant women, and whether these Web sites specifically target adolescents. A survey of sexual health information presented on the Web sites of crisis pregnancy centers. Internet. Crisis pregnancy center Web sites. Evaluation of the sexual health information presented on crisis pregnancy center Web sites. Themes included statements that condoms are not effective, promotion of abstinence-only education, availability of comprehensive sexual education, appeal to a young audience, provision of comprehensive sexual health information, and information about sexually transmitted infections (STIs). Crisis pregnancy center Web sites provide inaccurate and misleading information about condoms, STIs, and methods to prevent STI transmission. This information might be particularly harmful to adolescents, who might be unable to discern the quality of sexual health information on crisis pregnancy center Web sites. Listing crisis pregnancy centers in state resource directories might lend legitimacy to the information on these Web sites. States should be discouraged from listing Web sites as an accurate source of information in their resource directories. Copyright © 2016 North American Society for Pediatric and Adolescent Gynecology. Published by Elsevier Inc. All rights reserved.

Marketing your medical practice with an effective web presence.

PubMed

Finch, Tammy

2004-01-01

The proliferation of the World Wide Web has provided an opportunity for medical practices to sell themselves through low-cost marketing on the Internet. A Web site is a quick and effective way to provide patients with up-to-date treatment and procedure information. This article provides suggestions on what to include on a medical practice's Web site, how the Web can assist office staff and physicians, and cost options for your Web site. The article also discusses design tips, such as Web-site optimization.

Depth-of-processing effects as college students use academic advising Web sites.

PubMed

Boatright-Horowitz, Su L; Langley, Michelle; Gunnip, Matthew

2009-06-01

This research examined students' cognitive and affective responses to an academic advising Web site. Specifically, we investigated whether exposure to our Web site increased student reports that they would access university Web sites to obtain various types of advising information. A depth-of-processing (DOP) manipulation revealed this effect as students engaged in semantic processing of Web content but not when they engaged in superficial examination of the physical appearance of the same Web site. Students appeared to scan online academic advising materials for information of immediate importance without noticing other information or hyperlinks (e.g., regarding internships and careers). Suggestions are presented for increasing the effectiveness of academic advising Web sites.

Presence of pro-tobacco messages on the Web.

PubMed

Hong, Traci; Cody, Michael J

2002-01-01

Ignored in the finalized Master Settlement Agreement (National Association of Attorneys General, 1998), the unmonitored, unregulated World Wide Web (Web) can operate as a major vehicle for delivering pro-tobacco messages, images, and products to millions of young consumers. A content analysis of 318 randomly sampled pro-tobacco Web sites revealed that tobacco has a pervasive presence on the Web, especially on e-commerce sites and sites featuring hobbies, recreation, and "fetishes." Products can be ordered online on nearly 50% of the sites, but only 23% of the sites included underage verification. Further, only 11% of these sites contain health warnings. Instead, pro-tobacco sites frequently associate smoking with "glamorous" and "alternative" lifestyles, and with images of young males and young (thin, attractive) females. Finally, many of the Web sites offered interactive site features that are potentially appealing to young Web users. Recommendations for future research and counterstrategies are discussed.

Improving Web Searches: Case Study of Quit-Smoking Web Sites for Teenagers

PubMed Central

Skinner, Harvey

2003-01-01

Background The Web has become an important and influential source of health information. With the vast number of Web sites on the Internet, users often resort to popular search sites when searching for information. However, little is known about the characteristics of Web sites returned by simple Web searches for information about smoking cessation for teenagers. Objective To determine the characteristics of Web sites retrieved by search engines about smoking cessation for teenagers and how information quality correlates with the search ranking. Methods The top 30 sites returned by 4 popular search sites in response to the search terms "teen quit smoking" were examined. The information relevance and quality characteristics of these sites were evaluated by 2 raters. Objective site characteristics were obtained using a page-analysis Web site. Results Only 14 of the 30 Web sites are of direct relevance to smoking cessation for teenagers. The readability of about two-thirds of the 14 sites is below an eighth-grade school level and they ranked significantly higher (Kendall rank correlation, tau = -0.39, P= .05) in search-site results than sites with readability above or equal to that grade level. Sites that ranked higher were significantly associated with the presence of e-mail address for contact (tau = -0.46, P= .01), annotated hyperlinks to external sites (tau = -0.39, P= .04), and the presence of meta description tag (tau = -0.48, P= .002). The median link density (number of external sites that have a link to that site) of the Web pages was 6 and the maximum was 735. A higher link density was significantly associated with a higher rank (tau = -0.58, P= .02). Conclusions Using simple search terms on popular search sites to look for information on smoking cessation for teenagers resulted in less than half of the sites being of direct relevance. To improve search efficiency, users could supplement results obtained from simple Web searches with human-maintained Web directories and learn to refine their searches with more advanced search syntax. PMID:14713656

Perthes Disease: The Quality and Reliability of Information on the Internet.

PubMed

Nassiri, Mujtaba; Bruce-Brand, Robert A; O'Neill, Francis; Chenouri, Shojaeddin; Curtin, Paul

2015-01-01

Research has shown that up to 89% of parents used the Internet to seek health information regarding their child's medical condition. Much of the information on the Internet is valuable; however, the quality of health information is variable and unregulated. The aim of this study was to evaluate the quality and content of information about Perthes disease on the Internet using recognized scoring systems, identification of quality markers, and describe a novel specific score. We searched the top 3 search engines (Google, Yahoo!, and Bing) for the following keywords: "Perthes disease." Forty-five unique Web sites were identified. The Web sites were then categorized by type and assessed using the DISCERN score, the Journal of the American Medical Association (JAMA) benchmark criteria, and a novel Perthes-specific Content score. The presence of the Health On the Net (HON) code, a reported quality assurance marker, was noted. Of the Web sites analyzed, the Majority were Governmental and Nonprofit Organizations (NPO) (37.8%), followed by commercial Web sites (22.2%). Only 6 of the Web sites were HONcode certified. The mean DISCERN score was 53.1 (SD=9.0). The Governmental and NPO Web sites had the highest overall DISCERN scores followed closely by Physician Web sites. The mean JAMA benchmark criteria score was 2.1 (SD=1.2). Nine Web sites had maximal scores and the Academic Web sites had the highest overall JAMA benchmark scores. DISCERN scores, JAMA benchmark scores, and Perthes-specific Content scores were all greater for Web sites that bore the HONcode seal. The quality of information available online regarding Perthes disease is of variable quality. Governmental and NPO Web sites predominate and also provide higher quality content. The HONcode seal is a reliable indicator of Web site quality. Physicians should recommend the HONcode seal to their patients as a reliable indicator of Web site quality or, better yet, refer patients to sites they have personally reviewed. Supplying parents with a guide to health information on the Internet will help exclude Web sites as sources of misinformation.

Beyond Description: Converting Web Site Usage Statistics into Concrete Site Improvement Ideas

ERIC Educational Resources Information Center

Arendt, Julie; Wagner, Cassie

2010-01-01

Web site usage statistics are a widely used tool for Web site development, but libraries are still learning how to use them successfully. This case study summarizes how Morris Library at Southern Illinois University Carbondale implemented Google Analytics on its Web site and used the reports to inform a site redesign. As the main campus library at…

Elusive or Illuminating: Using the Web To Explore the Salem Witchcraft Trials.

ERIC Educational Resources Information Center

Hurter, Stephanie R.

2003-01-01

Presents Web sites useful for teaching about the Salem (Massachusetts) witchcraft trials. Includes Web sites that offer primary source material, collections of Web sites, teaching material, and sites that are interactive, including features, such as QuickTime movies. (CMK)

Web Site Design Benchmarking within Industry Groups.

ERIC Educational Resources Information Center

Kim, Sung-Eon; Shaw, Thomas; Schneider, Helmut

2003-01-01

Discussion of electronic commerce focuses on Web site evaluation criteria and applies them to different industry groups in Korea. Defines six categories of Web site evaluation criteria: business function, corporate credibility, contents reliability, Web site attractiveness, systematic structure, and navigation; and discusses differences between…

BLM Communications Use Lease to USAF to Conduct Patriot Communications Exercises in Lincoln County, Nevada. Final Environmental Assessment

DTIC Science & Technology

2008-08-01

pass, Mojave Desert Scrub intergrades with Basin communities and supports small components of Joshua tree, banana yucca (Y. baccata), and beavertail...Exercises in Lincoln County, Nevada 3-8 August 2008 Final EA ADA Site Location Land Type Land Cover Characteristics without flowers or seed pods was...Ackerman milkvetch (Astragalus ackermanii), Peck Station milkvetch (Astragalus eurylobus), Beatley’s phacelia (Phacelia beatleyae), wax flower (Jamesia

The effects of Web site structure: the role of personal difference.

PubMed

Chung, Hwiman; Ahn, Euijin

2007-12-01

This study examined the effects of Web site structures in terms of advertising effectiveness- memory, attitude, and behavioral intentions. The primary research question for this study is, What type of Web site (Web ad) structure is most effective? In the pilot study, we tested the difference between two Web site structures, linear and interactive, in terms of traditional advertising effectiveness. Results from the pilot study did not support our research expectations. However, differences in terms of memory were noted between the two structures. After re-creating the Web site based on subjects' comments, in the final experiment, we examined the differences between the two structures and the moderating role of personality difference on the effects of Web site structure. The results confirm that participants' attitude, memory, and behavioral intentions were affected differently by the different Web site structures. However, some research hypotheses were not supported by the current data.

The risk for developing cancer in Israeli ATM, BLM, and FANCC heterozygous mutation carriers.

PubMed

Laitman, Yael; Boker-Keinan, Lital; Berkenstadt, Michal; Liphsitz, Irena; Weissglas-Volkov, Daphna; Ries-Levavi, Liat; Sarouk, Ifat; Pras, Elon; Friedman, Eitan

2016-03-01

Cancer risks in heterozygous mutation carriers of the ATM, BLM, and FANCC genes are controversial. To shed light on this issue, cancer rates were evaluated by cross referencing asymptomatic Israeli heterozygous mutation carriers in the ATM, BLM, and FANCC genes with cancer diagnoses registered at the Israeli National Cancer Registry (INCR). Comparison of observed to expected Standardized Incidence Rates (SIR) was performed. Overall, 474 individuals participated in the study: 378 females; 25 Arab and 31 Jewish ATM carriers, 152 BLM carriers, and 170 FANCC carriers (all Ashkenazim). Age range at genotyping was 19-53 years (mean + SD 30.6 + 5 years). In addition, 96 males were included; 5, 34, and 57 ATM, BLM, and FANCC mutation carriers, respectively. Over 5-16 years from genotyping (4721 person/years), 15 new cancers were diagnosed in mutation carriers: 5 breast, 4 cervical, 3 melanomas, and one each bone sarcoma, pancreatic, and colorectal cancer. No single cancer diagnosis was more prevalent then expected in all groups combined or per gene analyzed. Specifically breast cancer SIR was 0.02-0.77. We conclude that Israeli ATM, BLM, and FANCC heterozygous mutation carriers are not at an increased risk for developing cancer. Copyright © 2015 Elsevier Inc. All rights reserved.

Double heterozygotes among breast cancer patients analyzed for BRCA1, CHEK2, ATM, NBN/NBS1, and BLM germ-line mutations.

PubMed

Sokolenko, Anna P; Bogdanova, Natalia; Kluzniak, Wojciech; Preobrazhenskaya, Elena V; Kuligina, Ekatherina S; Iyevleva, Aglaya G; Aleksakhina, Svetlana N; Mitiushkina, Natalia V; Gorodnova, Tatiana V; Bessonov, Alexandr A; Togo, Alexandr V; Lubiński, Jan; Cybulski, Cezary; Jakubowska, Anna; Dörk, Thilo; Imyanitov, Evgeny N

2014-06-01

17 double heterozygous (DH) breast cancer (BC) patients were identified upon the analysis of 5,391 affected women for recurrent Slavic mutations in BRCA1, CHEK2, NBN/NBS1, ATM, and BLM genes. Double heterozygosity was found for BRCA1 and BLM (4 patients), BRCA1 and CHEK2 (4 patients), CHEK2 and NBS1 (3 patients), BRCA1 and ATM (2 patients), CHEK2 and BLM (2 patients), CHEK2 and ATM (1 patient), and NBS1 and BLM (1 patient). DH BC patients were on average not younger than single mutation carriers and did not have an excess of bilateral BC; an additional non-breast tumor was documented in two BRCA1/BLM DH patients (ovarian cancer and lymphoplasmacytic lymphoma). Loss-of-heterozygosity (LOH) analysis of involved genes was performed in 5 tumors, and revealed a single instance of somatic loss of the wild-type allele (LOH at CHEK2 locus in BRCA1/CHEK2 double heterozygote). Distribution of mutations in patients and controls favors the hypothesis on multiplicative interaction between at least some of the analyzed genes. Other studies on double heterozygosity for BC-predisposing germ-line mutations are reviewed.

Efficacy of a Pilot Internet-Based Weight Management Program (H.E.A.L.T.H.) and Longitudinal Physical Fitness Data in Army Reserve Soldiers

PubMed Central

Newton, Robert L; Han, Hongmei; Stewart, Tiffany M; Ryan, Donna H; Williamson, Donald A

2011-01-01

Background The primary aims of this article are to describe the utilization of an Internet-based weight management Web site [Healthy Eating, Activity, and Lifestyle Training Headquarters (H.E.A.L.T.H.)] over a 12–27 month period and to describe concurrent weight and fitness changes in Army Reserve soldiers. Methods The H.E.A.L.T.H. Web site was marketed to Army Reserve soldiers via a Web site promotion program for 27 months (phase I) and its continued usage was observed over a subsequent 12-month period (phase II). Web site usage was obtained from the H.E.A.L.T.H. Web site. Weight and fitness data were extracted from the Regional Level Application Software (RLAS). Results A total of 1499 Army Reserve soldiers registered on the H.E.A.L.T.H. Web site. There were 118 soldiers who returned to the H.E.A.L.T.H. Web site more than once. Registration rate reduced significantly following the removal of the Web site promotion program. During phase I, 778 Army Reserve soldiers had longitudinal weight and fitness data in RLAS. Men exceeding the screening table weight gained less weight compared with men below it (p < .007). Percentage change in body weight was inversely associated with change in fitness scores. Conclusions The Web site promotion program resulted in 52% of available Army Reserve soldiers registering onto the H.E.A.L.T.H. Web site, and 7.9% used the Web site more than once. The H.E.A.L.T.H. Web site may be a viable population-based weight and fitness management tool for soldier use. PMID:22027327

Testosterone replacement therapy and the internet: an assessment of providers' health-related web site information content.

PubMed

Oberlin, Daniel T; Masson, Puneet; Brannigan, Robert E

2015-04-01

To compare how providers of testosterone replacement therapy (TRT) in large metropolitan cities promote androgen replacement on their patient-oriented Web sites. TRT provider Web sites were identified using Google search and the terms "Testosterone replacement" and the name of the 5 most populous US cities. These Web sites were assessed for (1) type or specialty of medical provider, (2) discussion of the benefits and risks of TRT, and (3) industry affiliations. In total, 75 Web sites were evaluated. Twenty-seven of the 75 clinics (36%) were directed by nonphysicians, 35 (47%) were overseen by nonurology or nonendocrine physicians, and only 13 (17%) were specialist managed. Fourteen of 75 (18.6%) Web sites disclosed industry relationships. Ninety-five percent of Web sites promoted the benefits of TRT including improved sex drive, cognitive improvement, increased muscle strength, and/or improved energy. Only 20 of 75 Web sites (26.6%) described any side effect of TRT. Web sites directed by specialists were twice as likely to discuss risks of TRT compared with nonspecialist providers (41% vs 20%; odds ratio = 2.77; P <.01). Nine of 75 (12%) of all Web sites actually refuted that TRT was associated with significant side effects. Urologists and endocrinologists are in the minority of providers promoting TRT on the Internet. Specialists are more likely to discuss risks associated with TRT although the majority of surveyed Web sites that promote TRT do not mention treatment risks. There is substantial variability in quality and quantity of information on provider Web sites, which may contribute to misinformation regarding this prevalent health issue. Copyright © 2015 Elsevier Inc. All rights reserved.

Structural features facilitating tumor cell targeting and internalization by bleomycin and its disaccharide.

PubMed

Yu, Zhiqiang; Paul, Rakesh; Bhattacharya, Chandrabali; Bozeman, Trevor C; Rishel, Michael J; Hecht, Sidney M

2015-05-19

We have shown previously that the bleomycin (BLM) carbohydrate moiety can recapitulate the tumor cell targeting effects of the entire BLM molecule, that BLM itself is modular in nature consisting of a DNA-cleaving aglycone which is delivered selectively to the interior of tumor cells by its carbohydrate moiety, and that there are disaccharides structurally related to the BLM disaccharide which are more efficient than the natural disaccharide at tumor cell targeting/uptake. Because BLM sugars can deliver molecular cargoes selectively to tumor cells, and thus potentially form the basis for a novel antitumor strategy, it seemed important to consider additional structural features capable of affecting the efficiency of tumor cell recognition and delivery. These included the effects of sugar polyvalency and net charge (at physiological pH) on tumor cell recognition, internalization, and trafficking. Since these parameters have been shown to affect cell surface recognition, internalization, and distribution in other contexts, this study has sought to define the effects of these structural features on tumor cell recognition by bleomycin and its disaccharide. We demonstrate that both can have a significant effect on tumor cell binding/internalization, and present data which suggests that the metal ions normally bound by bleomycin following clinical administration may significantly contribute to the efficiency of tumor cell uptake, in addition to their characterized function in DNA cleavage. A BLM disaccharide-Cy5** conjugate incorporating the positively charged dipeptide d-Lys-d-Lys was found to associate with both the mitochondria and the nuclear envelope of DU145 cells, suggesting possible cellular targets for BLM disaccharide-cytotoxin conjugates.

Structural Features Facilitating Tumor Cell Targeting and Internalization by Bleomycin and Its Disaccharide

PubMed Central

2016-01-01

We have shown previously that the bleomycin (BLM) carbohydrate moiety can recapitulate the tumor cell targeting effects of the entire BLM molecule, that BLM itself is modular in nature consisting of a DNA-cleaving aglycone which is delivered selectively to the interior of tumor cells by its carbohydrate moiety, and that there are disaccharides structurally related to the BLM disaccharide which are more efficient than the natural disaccharide at tumor cell targeting/uptake. Because BLM sugars can deliver molecular cargoes selectively to tumor cells, and thus potentially form the basis for a novel antitumor strategy, it seemed important to consider additional structural features capable of affecting the efficiency of tumor cell recognition and delivery. These included the effects of sugar polyvalency and net charge (at physiological pH) on tumor cell recognition, internalization, and trafficking. Since these parameters have been shown to affect cell surface recognition, internalization, and distribution in other contexts, this study has sought to define the effects of these structural features on tumor cell recognition by bleomycin and its disaccharide. We demonstrate that both can have a significant effect on tumor cell binding/internalization, and present data which suggests that the metal ions normally bound by bleomycin following clinical administration may significantly contribute to the efficiency of tumor cell uptake, in addition to their characterized function in DNA cleavage. A BLM disaccharide-Cy5** conjugate incorporating the positively charged dipeptide d-Lys-d-Lys was found to associate with both the mitochondria and the nuclear envelope of DU145 cells, suggesting possible cellular targets for BLM disaccharide–cytotoxin conjugates. PMID:25905565

Biomembrane disruption by silica-core nanoparticles: effect of surface functional group measured using a tethered bilayer lipid membrane

PubMed Central

Liu, Ying; Zhang, Zhen; Zhang, Quanxuan; Baker, Gregory L.; Worden, R. Mark

2013-01-01

Engineered nanomaterials (ENM) have desirable properties that make them well suited for many commercial applications. However, a limited understanding of how ENM’s properties influence their molecular interactions with biomembranes hampers efforts to design ENM that are both safe and effective. This paper describes the use of a tethered bilayer lipid membrane (tBLM) to characterize biomembrane disruption by functionalized silica-core nanoparticles. Electrochemical impedance spectroscopy was used to measure the time trajectory of tBLM resistance following nanoparticle exposure. Statistical analysis of parameters from an exponential resistance decay model was then used to quantify and analyze differences between the impedance profiles of nanoparticles that were unfunctionalized, amine-functionalized, or carboxyl-functionalized. All of the nanoparticles triggered a decrease in membrane resistance, indicating nanoparticle-induced disruption of the tBLM. Hierarchical clustering allowed the potency of nanoparticles for reducing tBLM resistance to be ranked in the order amine > carboxyl ~ bare silica. Dynamic light scattering analysis revealed that tBLM exposure triggered minor coalescence for bare and amine-functionalized silica nanoparticles but not for carboxyl-functionalized silica nanoparticles. These results indicate that the tBLM method can reproducibly characterize ENM-induced biomembrane disruption and can distinguish the BLM-disruption patterns of nanoparticles that are identical except for their surface functional groups. The method provides insight into mechanisms of molecular interaction involving biomembranes and is suitable for miniaturization and automation for high-throughput applications to help assess the health risk of nanomaterial exposure or identify ENM having a desired mode of interaction with biomembranes. PMID:24060565

The design and implementation of web mining in web sites security

NASA Astrophysics Data System (ADS)

Li, Jian; Zhang, Guo-Yin; Gu, Guo-Chang; Li, Jian-Li

2003-06-01

The backdoor or information leak of Web servers can be detected by using Web Mining techniques on some abnormal Web log and Web application log data. The security of Web servers can be enhanced and the damage of illegal access can be avoided. Firstly, the system for discovering the patterns of information leakages in CGI scripts from Web log data was proposed. Secondly, those patterns for system administrators to modify their codes and enhance their Web site security were provided. The following aspects were described: one is to combine web application log with web log to extract more information, so web data mining could be used to mine web log for discovering the information that firewall and Information Detection System cannot find. Another approach is to propose an operation module of web site to enhance Web site security. In cluster server session, Density-Based Clustering technique is used to reduce resource cost and obtain better efficiency.

Continued benefits of a technical assistance web site to local tobacco control coalitions during a state budget shortfall.

PubMed

Buller, David B; Young, Walter F; Bettinghaus, Erwin P; Borland, Ron; Walther, Joseph B; Helme, Donald; Andersen, Peter A; Cutter, Gary R; Maloy, Julie A

2011-01-01

A state budget shortfall defunded 10 local tobacco coalitions during a randomized trial but defunded coalitions continued to have access to 2 technical assistance Web sites. To test the ability of Web-based technology to provide technical assistance to local tobacco control coalitions. Randomized 2-group trial with local tobacco control coalitions as the unit of randomization. Local communities (ie, counties) within the State of Colorado. Leaders and members in 34 local tobacco control coalitions funded by the state health department in Colorado. Two technical assistance Web sites: A Basic Web site with text-based information and a multimedia Enhanced Web site containing learning modules, resources, and communication features. Use of the Web sites in minutes, pages, and session and evaluations of coalition functioning on coalition development, conflict resolution, leadership satisfaction, decision-making satisfaction, shared mission, personal involvement, and organization involvement in survey of leaders and members. Coalitions that were defunded but had access to the multimedia Enhanced Web site during the Fully Funded period and after defunding continued to use it (treatment group × funding status × period, F(3,714) = 3.18, P = .0234). Coalitions with access to the Basic Web site had low Web site use throughout and use by defunded coalitions was nearly zero when funding ceased. Members in defunded Basic Web site coalitions reported that their coalitions functioned worse than defunded Enhanced Web site coalitions (coalition development: group × status, F(1,360) = 4.81, P = .029; conflict resolution: group × status, F(1,306) = 5.69, P = .018; leadership satisfaction: group × status, F(1,342) = 5.69, P = .023). The Enhanced Web site may have had a protective effect on defunded coalitions. Defunded coalitions may have increased their capacity by using the Enhanced Web site when fully funded or by continuing to use the available online resources after defunding. Web-based technical assistance with online training and resources may be a good investment when future funding is not ensured.

Publicly available hospital comparison web sites: determination of useful, valid, and appropriate information for comparing surgical quality.

PubMed

Leonardi, Michael J; McGory, Marcia L; Ko, Clifford Y

2007-09-01

To explore hospital comparison Web sites for general surgery based on: (1) a systematic Internet search, (2) Web site quality evaluation, and (3) exploration of possible areas of improvement. A systematic Internet search was performed to identify hospital quality comparison Web sites in September 2006. Publicly available Web sites were rated on accessibility, data/statistical transparency, appropriateness, and timeliness. A sample search was performed to determine ranking consistency. Six national hospital comparison Web sites were identified: 1 government (Hospital Compare [Centers for Medicare and Medicaid Services]), 2 nonprofit (Quality Check [Joint Commission on Accreditation of Healthcare Organizations] and Hospital Quality and Safety Survey Results [Leapfrog Group]), and 3 proprietary sites (names withheld). For accessibility and data transparency, the government and nonprofit Web sites were best. For appropriateness, the proprietary Web sites were best, comparing multiple surgical procedures using a combination of process, structure, and outcome measures. However, none of these sites explicitly defined terms such as complications. Two proprietary sites allowed patients to choose ranking criteria. Most data on these sites were 2 years old or older. A sample search of 3 surgical procedures at 4 hospitals demonstrated significant inconsistencies. Patients undergoing surgery are increasingly using the Internet to compare hospital quality. However, a review of available hospital comparison Web sites shows suboptimal measures of quality and inconsistent results. This may be partially because of a lack of complete and timely data. Surgeons should be involved with quality comparison Web sites to ensure appropriate methods and criteria.

75 FR 6063 - Availability of NRC Open Government Web Site

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-05

... NUCLEAR REGULATORY COMMISSION [NRC-2010-0003] Availability of NRC Open Government Web Site AGENCY: Nuclear Regulatory Commission. ACTION: Notice of Availability of Open Government Web site for Online... Register notice, informs the public that the Nuclear Regulatory Commission's (NRC) Open Government Web site...

Hot Spots on the Web for Teacher Librarians: A Selection of Recommended Web Sites for TLs To Visit.

ERIC Educational Resources Information Center

1996

Six papers review and recommend sites on the Web as resources for teacher librarians include: "Just Do It: A Guide to Getting Out There and Doing It Yourself" (Catherine Ryan); "A Selection of Recommended Web Sites for TLs To Visit" (Karen Bonanno); "A Selection of Recommended Web Sites for TLs To Visit" (Sandra…

The Atlas of Chinese World Wide Web Ecosystem Shaped by the Collective Attention Flows.

PubMed

Lou, Xiaodan; Li, Yong; Gu, Weiwei; Zhang, Jiang

2016-01-01

The web can be regarded as an ecosystem of digital resources connected and shaped by collective successive behaviors of users. Knowing how people allocate limited attention on different resources is of great importance. To answer this, we embed the most popular Chinese web sites into a high dimensional Euclidean space based on the open flow network model of a large number of Chinese users' collective attention flows, which both considers the connection topology of hyperlinks between the sites and the collective behaviors of the users. With these tools, we rank the web sites and compare their centralities based on flow distances with other metrics. We also study the patterns of attention flow allocation, and find that a large number of web sites concentrate on the central area of the embedding space, and only a small fraction of web sites disperse in the periphery. The entire embedding space can be separated into 3 regions(core, interim, and periphery). The sites in the core (1%) occupy a majority of the attention flows (40%), and the sites (34%) in the interim attract 40%, whereas other sites (65%) only take 20% flows. What's more, we clustered the web sites into 4 groups according to their positions in the space, and found that similar web sites in contents and topics are grouped together. In short, by incorporating the open flow network model, we can clearly see how collective attention allocates and flows on different web sites, and how web sites connected each other.

Navigating the high seas of Federal Programs to ensure usable science delivery

NASA Astrophysics Data System (ADS)

Bachelet, D. M.; Gough, M.; Baker, B.; Sheehan, T.; Mutch, T.; Brown, M.

2016-12-01

Conservation Biology Institute (CBI) has been developing web applications to serve credible and usable information that allows land stewards, as well as the general public, to better understand the challenges posed by on-going environmental change. Working with 18 Landscape Conservation Cooperatives (LCCs), CBI has developed Conservation Atlases that allow users to visualize regional spatial data. Some include specific tools such as the Conservation Blueprint in the South Atlantic LCC conservation atlas which is a living spatial plan that identifies priority areas for shared conservation action based on ecosystem indicator condition and connectivity. More than 400 people from over 100 different organizations have actively participated in its development so far. CBI worked closely with 4 LCCs to design a Landscape Climate Dashboard (http://bit.ly/2atu8Df) that provides CMIP5 climate projections averaged over federally and tribally protected lands in the western US. With support from BLM and Great Basin LCC, a Sagebrush Climate Console provides managers short-term NOAA forecasts, CMIP5 climate projections, regional intactness and site sensitivity over sagebrush extent and grouse range. The USDA Pacific Northwest Climate Hub and the US Forest Service are currently funding a collaborative effort between CBI programmers, USFS and Oregon State University forest geneticists to develop a seedlot source tool for the conterminous US and Alaska. This tool has inspired Great Basin sagebrush restoration scientists who have collected information on seed provenance and will hopefully soon collaborate to help develop the first seed source tool for sagebrush managers. Furthermore, with support from USDA Northwest Climate Hub, CBI's Data Basin site (http://bit.ly/2aLRlhZ) is providing downscaled CMIP5 climate projections and impacts from a Climate Science Center and NOAA Regional Integrated Science Assessment funded project that was the result of a collaborative effort between Oregon State University, University of Idaho, and the University of Washington. With much experience with local and regional managers and federal programs we will show examples of exciting successes and report on some challenges in coordinating exchanges and extracting valuable feedback to improve the usability of web applications.

A Web-based approach to blood donor preparation.

PubMed

France, Christopher R; France, Janis L; Kowalsky, Jennifer M; Copley, Diane M; Lewis, Kristin N; Ellis, Gary D; McGlone, Sarah T; Sinclair, Kadian S

2013-02-01

Written and video approaches to donor education have been shown to enhance donation attitudes and intentions to give blood, particularly when the information provides specific coping suggestions for donation-related concerns. This study extends this work by comparing Web-based approaches to donor preparation among donors and nondonors. Young adults (62% female; mean [±SD] age, 19.3 [±1.5] years; mean [range] number of prior blood donations, 1.1 [0-26]; 60% nondonors) were randomly assigned to view 1) a study Web site designed to address common blood donor concerns and suggest specific coping strategies (n = 238), 2) a standard blood center Web site (n = 233), or 3) a control Web site where participants viewed videos of their choice (n = 202). Measures of donation attitude, anxiety, confidence, intention, anticipated regret, and moral norm were completed before and after the intervention. Among nondonors, the study Web site produced greater changes in donation attitude, confidence, intention, and anticipated regret relative to both the standard and the control Web sites, but only differed significantly from the control Web site for moral norm and anxiety. Among donors, the study Web site produced greater changes in donation confidence and anticipated regret relative to both the standard and the control Web sites, but only differed significantly from the control Web site for donation attitude, anxiety, intention, and moral norm. Web-based donor preparation materials may provide a cost-effective way to enhance donation intentions and encourage donation behavior. © 2012 American Association of Blood Banks.

Web Analytics: A Picture of the Academic Library Web Site User

ERIC Educational Resources Information Center

Black, Elizabeth L.

2009-01-01

This article describes the usefulness of Web analytics for understanding the users of an academic library Web site. Using a case study, the analysis describes how Web analytics can answer questions about Web site user behavior, including when visitors come, the duration of the visit, how they get there, the technology they use, and the most…

Quality and accuracy of sexual health information web sites visited by young people.

PubMed

Buhi, Eric R; Daley, Ellen M; Oberne, Alison; Smith, Sarah A; Schneider, Tali; Fuhrmann, Hollie J

2010-08-01

We assessed online sexual health information quality and accuracy and the utility of web site quality indicators. In reviewing 177 sexual health web sites, we found below average quality but few inaccuracies. Web sites with the most technically complex information and/or controversial topics contained the most inaccuracies. We found no association between inaccurate information and web site quality. (c) 2010 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.

Quality of prostate cancer screening information on the websites of nationally recognized cancer centers and health organizations.

PubMed

Manole, Bogdan-Alexandru; Wakefield, Daniel V; Dove, Austin P; Dulaney, Caleb R; Marcrom, Samuel R; Schwartz, David L; Farmer, Michael R

2017-12-24

The purpose of this study was to survey the accessibility and quality of prostate-specific antigen (PSA) screening information from National Cancer Institute (NCI) cancer center and public health organization Web sites. We surveyed the December 1, 2016, version of all 63 NCI-designated cancer center public Web sites and 5 major online clearinghouses from allied public/private organizations (cancer.gov, cancer.org, PCF.org, USPSTF.org, and CDC.gov). Web sites were analyzed according to a 50-item list of validated health care information quality measures. Web sites were graded by 2 blinded reviewers. Interrater agreement was confirmed by Cohen kappa coefficient. Ninety percent of Web sites addressed PSA screening. Cancer center sites covered 45% of topics surveyed, whereas organization Web sites addressed 70%. All organizational Web pages addressed the possibility of false-positive screening results; 41% of cancer center Web pages did not. Forty percent of cancer center Web pages also did not discuss next steps if a PSA test was positive. Only 6% of cancer center Web pages were rated by our reviewers as "superior" (eg, addressing >75% of the surveyed topics) versus 20% of organizational Web pages. Interrater agreement between our reviewers was high (kappa coefficient = 0.602). NCI-designated cancer center Web sites publish lower quality public information about PSA screening than sites run by major allied organizations. Nonetheless, information and communication deficiencies were observed across all surveyed sites. In an age of increasing patient consumerism, prospective prostate cancer patients would benefit from improved online PSA screening information from provider and advocacy organizations. Validated cancer patient Web educational standards remain an important, understudied priority. Copyright © 2018. Published by Elsevier Inc.

Tobacco-prevention messages online: social marketing via the Web.

PubMed

Lin, Carolyn A; Hullman, Gwen A

2005-01-01

Antitobacco groups have joined millions of other commercial or noncommercial entities in developing a presence on the Web. These groups primarily represent the following different sponsorship categories: grassroots, medical, government, and corporate. To obtain a better understanding of the strengths and weaknesses in the message design of antitobacco Web sites, this project analyzed 100 antitobacco Web sites ranging across these four sponsorship categories. The results show that the tobacco industry sites posted just enough antismoking information to appease the antismoking publics. Medical organizations designed their Web sites as specialty sites and offered mostly scientific information. While the government sites resembled a clearinghouse for antitobacco related information, the grassroots sites represented the true advocacy outlets. In general, the industry sites provided the weakest persuasive messages and medical sites fared only slightly better. Government and grassroots sites rated most highly in presenting their antitobacco campaign messages on the Web.

78 FR 54241 - Proposed Information Collection; Comment Request; BroadbandMatch Web Site Tool

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-03

... Information Collection; Comment Request; BroadbandMatch Web Site Tool AGENCY: National Telecommunications and... goal of increased broadband deployment and use in the United States. The BroadbandMatch Web site began... empowering technology effectively. II. Method of Collection BroadbandMatch users access the Web site through...

75 FR 75170 - APHIS User Fee Web Site

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-02

...] APHIS User Fee Web Site AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Notice... recover the costs of providing certain services. This notice announces the availability of a Web site that contains information about the Agency's user fees. ADDRESSES: The Agency's user fee Web site is located at...

78 FR 76187 - 30-Day Notice of Proposed Information Collection: Exchange Programs Alumni Web Site Registration

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-16

...: Exchange Programs Alumni Web Site Registration ACTION: Notice of request for public comment and submission... Information Collection: Exchange Programs Alumni Web site Registration. OMB Control Number: 1405-0192. Type of... proposed collection: The International Exchange Alumni Web site requires information to process users...

12 CFR 555.310 - How do I notify OTS?

Code of Federal Regulations, 2010 CFR

2010-01-01

...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

7 CFR 2902.6 - Providing product information to Federal agencies.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Web site. An informational USDA Web site implementing section 9002 can be found at: http://www.biobased.oce.usda.gov. USDA will maintain a voluntary Web-based information site for manufacturers and... information. This Web site will provide information as to the availability, relative price, biobased content...

49 CFR 604.16 - Duties for recipients with respect to charter registration Web site.

Code of Federal Regulations, 2010 CFR

2010-10-01

... registration Web site. 604.16 Section 604.16 Transportation Other Regulations Relating to Transportation... Qualified Human Service Organizations and Duties for Recipients With Respect to Charter Registration Web site § 604.16 Duties for recipients with respect to charter registration Web site. Each recipient shall...

7 CFR 3201.6 - Providing product information to Federal agencies.

Code of Federal Regulations, 2012 CFR

2012-01-01

...) Informational Web site. An informational USDA Web site implementing section 9002 can be found at: http://www.biopreferred.gov. USDA will maintain a voluntary Web-based information site for manufacturers and vendors of... Web site will provide information as to the availability, relative price, biobased content...

7 CFR 3201.6 - Providing product information to Federal agencies.

Code of Federal Regulations, 2014 CFR

2014-01-01

...) Informational Web site. An informational USDA Web site implementing section 9002 can be found at: http://www.biopreferred.gov. USDA will maintain a voluntary Web-based information site for manufacturers and vendors of... Web site will provide information as to the availability, relative price, biobased content...

A Design Analysis Model for Developing World Wide Web Sites.

ERIC Educational Resources Information Center

Ma, Yan

2002-01-01

Examines the relationship between and among designers, text, and users of the Galter Health Sciences Library Web site at Northwestern University by applying reader-response criticism. Highlights include Web site design; comparison of designers' intentions with the actual organization of knowledge on the Web site; and compares designer's intentions…

49 CFR 604.16 - Duties for recipients with respect to charter registration Web site.

Code of Federal Regulations, 2013 CFR

2013-10-01

... registration Web site. 604.16 Section 604.16 Transportation Other Regulations Relating to Transportation... Qualified Human Service Organizations and Duties for Recipients With Respect to Charter Registration Web site § 604.16 Duties for recipients with respect to charter registration Web site. Each recipient shall...

49 CFR 604.16 - Duties for recipients with respect to charter registration Web site.

Code of Federal Regulations, 2011 CFR

2011-10-01

... registration Web site. 604.16 Section 604.16 Transportation Other Regulations Relating to Transportation... Qualified Human Service Organizations and Duties for Recipients With Respect to Charter Registration Web site § 604.16 Duties for recipients with respect to charter registration Web site. Each recipient shall...

12 CFR 555.310 - How do I notify OTS?

Code of Federal Regulations, 2013 CFR

2013-01-01

...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

12 CFR 555.310 - How do I notify OTS?

Code of Federal Regulations, 2012 CFR

2012-01-01

...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

49 CFR 604.16 - Duties for recipients with respect to charter registration Web site.

Code of Federal Regulations, 2014 CFR

2014-10-01

... registration Web site. 604.16 Section 604.16 Transportation Other Regulations Relating to Transportation... Qualified Human Service Organizations and Duties for Recipients With Respect to Charter Registration Web site § 604.16 Duties for recipients with respect to charter registration Web site. Each recipient shall...

49 CFR 604.16 - Duties for recipients with respect to charter registration Web site.

Code of Federal Regulations, 2012 CFR

2012-10-01

... registration Web site. 604.16 Section 604.16 Transportation Other Regulations Relating to Transportation... Qualified Human Service Organizations and Duties for Recipients With Respect to Charter Registration Web site § 604.16 Duties for recipients with respect to charter registration Web site. Each recipient shall...

12 CFR 555.310 - How do I notify OTS?

Code of Federal Regulations, 2014 CFR

2014-01-01

...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

Beta-test Results for an HPV Information Web site: GoHealthyGirls.org – Increasing HPV Vaccine Uptake in the United States

PubMed Central

Nodulman, Jessica A.; Kong, Alberta S.; Wheeler, Cosette M.; Buller, David B.; Woodall, W. Gill

2014-01-01

A web site, GoHealthyGirls, was developed to educate and inform parents and their adolescent daughters about human papillomavirus (HPV) and HPV vaccines. This article provides an overview of web site development and content followed by the results of a beta-test of the web site. 63 New Mexican parents of adolescent girls tested the site. Results indicated that GoHealthyGirls was a functioning and appealing web site. During this brief educational intervention, findings suggest that the web site has the potential to increase HPV vaccine uptake. This research supports the Internet as a valuable channel to disseminate health education and information to diverse populations. PMID:25221442

Corporate Web Sites in Traditional Print Advertisements.

ERIC Educational Resources Information Center

Pardun, Carol J.; Lamb, Larry

1999-01-01

Describes the Web presence in print advertisements to determine how marketers are creating bridges between traditional advertising and the Internet. Content analysis showed Web addresses in print ads; categories of advertisers most likely to link print ads with Web sites; and whether the Web site attempts to develop a database of potential…

Side by Side: What a Comparative Usability Study Told Us about a Web Site Redesign

ERIC Educational Resources Information Center

Dougan, Kirstin; Fulton, Camilla

2009-01-01

Library Web sites must compete against easy-to-use sites, such as Google Scholar, Google Books, and Wikipedia, for students' time and attention. Library Web sites must therefore be designed with aesthetics and user perceptions at the forefront. The Music and Performing Arts Library at Urbana-Champaign's Web site was overcrowded and in much need of…

The Atlas of Chinese World Wide Web Ecosystem Shaped by the Collective Attention Flows

PubMed Central

Lou, Xiaodan; Li, Yong; Gu, Weiwei; Zhang, Jiang

2016-01-01

The web can be regarded as an ecosystem of digital resources connected and shaped by collective successive behaviors of users. Knowing how people allocate limited attention on different resources is of great importance. To answer this, we embed the most popular Chinese web sites into a high dimensional Euclidean space based on the open flow network model of a large number of Chinese users’ collective attention flows, which both considers the connection topology of hyperlinks between the sites and the collective behaviors of the users. With these tools, we rank the web sites and compare their centralities based on flow distances with other metrics. We also study the patterns of attention flow allocation, and find that a large number of web sites concentrate on the central area of the embedding space, and only a small fraction of web sites disperse in the periphery. The entire embedding space can be separated into 3 regions(core, interim, and periphery). The sites in the core (1%) occupy a majority of the attention flows (40%), and the sites (34%) in the interim attract 40%, whereas other sites (65%) only take 20% flows. What’s more, we clustered the web sites into 4 groups according to their positions in the space, and found that similar web sites in contents and topics are grouped together. In short, by incorporating the open flow network model, we can clearly see how collective attention allocates and flows on different web sites, and how web sites connected each other. PMID:27812133

Testing of a prototype Web based intervention for adolescent mothers on postpartum depression.

PubMed

Logsdon, M Cynthia; Barone, Michael; Lynch, Tania; Robertson, Ashley; Myers, John; Morrison, David; York, Sara; Gregg, Jennifer

2013-08-01

This article describes testing of a prototype Web site for adolescent mothers with postpartum depression; providing proof of concept. Participants (N=138) were recruited from a public school-based program for adolescent parents and completed the Mental Health Acceptability Scale, Stigma Scale for Receiving Psychological Help, and Attitudes Towards Seeking Professional Psychological Help Scale before, and after, the Web site intervention. They also provided feedback on the usability of the Web site. Attitudes related to depression and treatment (ATSPPH) improved after viewing the Web site (p=.023). Feedback on the Web site indicated that it was easy to use (77%), reflecting highly acceptable score for product usability. The data provide the foundation for the launch of the Web site from prototype to product and more comprehensive testing. The creation and testing of informational text messages will be added to the Web site to increase the interactivity and dose of the intervention. Copyright © 2013 Elsevier Inc. All rights reserved.

Drill baby drill: An analysis of how energy development displaced ranching's dominance over the BLM's subgovernment policymaking environment

NASA Astrophysics Data System (ADS)

Forbis, Robert Earl, Jr.

Academic literature analyzing the Bureau of Land Management (BLM) land-use subgovernment stops at the Taylor Grazing Act and concludes that the historical development of administering grazing on public lands led to the capture of the BLM by ranching interests. Using a two-pronged methodological approach of process tracing and elite interviews this dissertation seeks to advance our collective knowledge of subgovernment theory by (a) clarifying the impact executive decision-making has on subgovernments and (b) identifying the conditions under which strategically competitive behavior between two competing subgovernment actors occurs. The dissertation seeks to update the literature by explaining what has caused the BLM to shift from a rancher-dominated agency to an energy dominated agency by identifying conditions under which subgovernment actors strategically respond to a political conflict. The research poses two questions: (1) how have executive actions disrupted an existing balance of power in a so-called "strong corner" of an entrenched subgovernment system and (2) what happens when conflict and competition break out between allied members of the system? Analysis indicates that as the BLM responded to Executive actions emphasizing domestic energy production, a conflict emerged between traditional allies: ranching and energy. Triggered by the unintended consequence of awakening long-dormant legislation, split-estate energy development---where property rights are severed between private surface and federal mineral estates---expanded across the West. In turn, this expansion helped establish the conditions for conflict and in doing so disrupted the balance of power between large public resource use interests in the relatively stable land-use subgovernment of the BLM. Indicative of energy's emerging dominance of the BLM's subgovernment, split-estate energy development led ranching interests to seek the protection of their Western state legislatures. This shift in domination led to a series of fiercely competitive political responses between the formerly allied interest groups. I argue that as political conflict intensified it is clear that the BLM's land-use policies are no longer dominated by ranching interests, but are now dominated by energy development interests. The analysis concludes that this shift in domination disproves the long accepted conclusion that the BLM is forever an agency captured by ranching interests.

Framing medical tourism: an examination of appeal, risk, convalescence, accreditation, and interactivity in medical tourism web sites.

PubMed

Mason, Alicia; Wright, Kevin B

2011-02-01

This exploratory study analyzed the content of medical tourism Web sites in an attempt to examine how they convey information about benefits and risks of medical procedures, how they frame credibility, and the degree to which these Web sites include interactive features for consumers. Drawing upon framing theory, the researchers content analyzed a sample of 66 medical tourism Web sites throughout the world. The results indicated that medical tourism Web sites largely promote the benefits of medical procedures while downplaying the risks, and relatively little information regarding the credibility of these services appears. In addition, the presentation of benefits/risks, credibility, and Web site interactivity were found to differ by region and type of facility. The authors discuss the implications of these findings concerning the framing of medical tourism Web site content, future directions for research, and limitations.

Original Research: ACE2 activator associated with physical exercise potentiates the reduction of pulmonary fibrosis

PubMed Central

Prata, Luana O; Rodrigues, Carolina R; Martins, Jéssica M; Vasconcelos, Paula C; Oliveira, Fabrício Marcus S; Ferreira, Anderson J; Rodrigues-Machado, Maria da Glória

2016-01-01

The interstitial lung diseases are poorly understood and there are currently no studies evaluating the association of physical exercise with an ACE2 activator (DIZE) as a possible treatment for this group of diseases. We evaluate the effects of pharmacological treatment with an angiotensin-converting enzyme 2 activator drug, associated with exercise, on the pulmonary lesions induced by bleomycin. From the 96 male Balb/c mice used in the experiment, only 49 received 8 U/kg of bleomycin (BLM, intratracheally). The mice were divided into control (C) and bleomycin (BLM) groups, sedentary and trained (C-SED, C-EXE, BLM-SED, BLM-EXE), control and bleomycin and also sedentary and trained treated with diminazene (C-SED/E, C-EXE/E, BLM-SED/E, BLM-EXE/E). The animals were trained five days/week, 1 h/day with 60% of the maximum load obtained in a functional capacity test, for four weeks. Diminazene groups were treated (1 mg/kg, by gavage) daily until the end of the experiment. The lungs were collected 48 h after the training program, set in buffered formalin and investigated by Gomori’s trichrome, immunohistochemistry of collagen type I, TGF-β1, beta-prolyl-4-hydroxylase, MMP-1 and -2. The BLM-EXE/E group obtained a significant increase in functional capacity, reduced amount of fibrosis and type I collagen, decreased expression of TGF-β1 and beta-prolyl-4-hydroxylase and an increase of metalloproteinase −1, −2 when compared with the other groups. The present research shows, for the first time, that exercise training associated with the activation of ACE2 potentially reduces pulmonary fibrosis. PMID:27550926

Inhibition of bleomycin-induced pulmonary fibrosis by bone marrow-derived mesenchymal stem cells might be mediated by decreasing MMP9, TIMP-1, INF-γ and TGF-β.

PubMed

Yu, Shi-huan; Liu, Li-jie; Lv, Bin; Che, Chun-li; Fan, Da-ping; Wang, Li-feng; Zhang, Yi-mei

2015-08-01

The study was aimed to investigate the mechanism and administration timing of bone marrow-derived mesenchymal stem cells (BMSCs) in bleomycin (BLM)-induced pulmonary fibrosis mice. Thirty-six mice were divided into six groups: control group (saline), model group (intratracheal administration of BLM), day 1, day 3 and day 6 BMSCs treatment groups and hormone group (hydrocortisone after BLM treatment). BMSCs treatment groups received BMSCs at day 1, 3 or 6 following BLM treatment, respectively. Haematoxylin and eosin and Masson staining were conducted to measure lung injury and fibrosis, respectively. Matrix metalloproteinase (MMP9), tissue inhibitor of metalloproteinase-1 (TIMP-1), γ-interferon (INF-γ) and transforming growth factor β1 (TGF-β) were detected in both lung tissue and serum. Histologically, the model group had pronounced lung injury, increased inflammatory cells and collagenous fibres and up-regulated MMP9, TIMP-1, INF-γ and TGF-β compared with control group. The histological appearance of lung inflammation and fibrosis and elevation of these parameters were inhibited in BMSCs treatment groups, among which, day 3 and day 6 treatment groups had less inflammatory cells and collagenous fibres than day 1 treatment group. BMSCs might suppress lung fibrosis and inflammation through down-regulating MMP9, TIMP-1, INF-γ and TGF-β. Delayed BMSCs treatment might exhibit a better therapeutic effect. Highlights are as follows: 1. BMSCs repair lung injury induced by BLM. 2. BMSCs attenuate pulmonary fibrosis induced by BLM. 3. BMSCs transplantation down-regulates MMP9 and TIMP-1. 4. BMSCs transplantation down-regulates INF-γ and TGF-β. 5. Delayed transplantation timing of BMSCs might exhibit a better effect against BLM. Copyright © 2015 John Wiley & Sons, Ltd.

A multilingual assessment of melanoma information quality on the Internet.

PubMed

Bari, Lilla; Kemeny, Lajos; Bari, Ferenc

2014-06-01

This study aims to assess and compare melanoma information quality in Hungarian, Czech, and German languages on the Internet. We used country-specific Google search engines to retrieve the first 25 uniform resource locators (URLs) by searching the word "melanoma" in the given language. Using the automated toolbar of Health On the Net Foundation (HON), we assessed each Web site for HON certification based on the Health On the Net Foundation Code of Conduct (HONcode). Information quality was determined using a 35-point checklist created by Bichakjian et al. (J Clin Oncol 20:134-141, 2002), with the NCCN melanoma guideline as control. After excluding duplicate and link-only pages, a total of 24 Hungarian, 18 Czech, and 21 German melanoma Web sites were evaluated and rated. The amount of HON certified Web sites was the highest among the German Web pages (19%). One of the retrieved Hungarian and none of the Czech Web sites were HON certified. We found the highest number of Web sites containing comprehensive, correct melanoma information in German language, followed by Czech and Hungarian pages. Although the majority of the Web sites lacked data about incidence, risk factors, prevention, treatment, work-up, and follow-up, at least one comprehensive, high-quality Web site was found in each language. Several Web sites contained incorrect information in each language. While a small amount of comprehensive, quality melanoma-related Web sites was found, most of the retrieved Web content lacked basic disease information, such as risk factors, prevention, and treatment. A significant number of Web sites contained malinformation. In case of melanoma, primary and secondary preventions are of especially high importance; therefore, the improvement of disease information quality available on the Internet is necessary.

5 CFR 2604.201 - Public reading room facility and Web site.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Public reading room facility and Web site... DISCLOSURE REPORTS FOIA Public Reading Room Facility and Web Site; Index Identifying Information for the Public § 2604.201 Public reading room facility and Web site. (a)(1) Location of public reading room...

Analysis of Elementary School Web Sites

ERIC Educational Resources Information Center

Hartshorne, Richard; Friedman, Adam; Algozzine, Bob; Kaur, Daljit

2008-01-01

While researchers have studied the use and value of educational software for many years, study of school Web sites and/or their effectiveness is limited. In this investigation, we identified goals and functions of school Web sites and used the foundations of effective Web site design to develop an evaluation checklist. We then applied these…

Academic Library Web Sites: Current Practice and Future Directions

ERIC Educational Resources Information Center

Detlor, Brian; Lewis, Vivian

2006-01-01

To address competitive threats, academic libraries are encouraged to build robust Web sites personalized to learning and research tasks. Through an evaluation of Association of Research Libraries (ARL)-member Web sites, we suggest how library Web sites should evolve and reflect upon the impacts such recommendations may have on academic libraries…

75 FR 22391 - Notice of Web Site Publication for the Climate Program Office

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-28

...-01] Notice of Web Site Publication for the Climate Program Office AGENCY: Climate Program Office (CPO... its Web site at http://www.climate.noaa.gov . FOR FURTHER INFORMATION CONTACT: Eric Locklear; Chief... information is available on the Climate Program Office Web site pertaining to the CPO's research strategies...

75 FR 66413 - 30-Day Notice of Proposed Information Collection: Exchange Programs Alumni Web Site Registration...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-28

...: Exchange Programs Alumni Web Site Registration, DS-7006 ACTION: Notice of request for public comment and... Collection The Exchange Programs Alumni Web site requires information to process users' voluntary requests for participation in the Web site. Other than contact information, which is required for website...

78 FR 66420 - Proposed Enhancements to the Motor Carrier Safety Measurement System (SMS) Public Web Site

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-05

...-0392] Proposed Enhancements to the Motor Carrier Safety Measurement System (SMS) Public Web Site AGENCY... on the Agency's Safety Measurement System (SMS) public Web site. FMCSA first announced the... public Web site that are the direct result of feedback from stakeholders regarding the information...

77 FR 38033 - Notice of Establishment of a Commodity Import Approval Process Web Site

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-26

... Process Web Site AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Notice. SUMMARY: We are announcing the creation of a new Plant Protection and Quarantine Web site that will provide stakeholders with... comment on draft risk assessments. This Web site will make the commodity import approval process more...

22 CFR 181.9 - Internet Web site publication.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty or...

22 CFR 181.9 - Internet Web site publication.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty or...

22 CFR 181.9 - Internet Web site publication.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty or...

Characteristics of Food Industry Web Sites and "Advergames" Targeting Children

ERIC Educational Resources Information Center

Culp, Jennifer; Bell, Robert A.; Cassady, Diana

2010-01-01

Objective: To assess the content of food industry Web sites targeting children by describing strategies used to prolong their visits and foster brand loyalty; and to document health-promoting messages on these Web sites. Design: A content analysis was conducted of Web sites advertised on 2 children's networks, Cartoon Network and Nickelodeon. A…

5 CFR 2604.201 - Public reading room facility and Web site.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Public reading room facility and Web site... DISCLOSURE REPORTS FOIA Public Reading Room Facility and Web Site; Index Identifying Information for the Public § 2604.201 Public reading room facility and Web site. (a)(1) Location of public reading room...

Library Web Sites in Pakistan: An Analysis of Content

ERIC Educational Resources Information Center

Qutab, Saima; Mahmood, Khalid

2009-01-01

Purpose: The purpose of this paper is to investigate library web sites in Pakistan, to analyse their content and navigational strengths and weaknesses and to give recommendations for developing better web sites and quality assessment studies. Design/methodology/approach: Survey of web sites of 52 academic, special, public and national libraries in…

22 CFR 181.9 - Internet Web site publication.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty or...

22 CFR 181.9 - Internet Web site publication.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty or...

Formative Evaluation of a Family Life Education Web Site

ERIC Educational Resources Information Center

Steimle, Brynn M.; Duncan, Stephen F.

2004-01-01

Hundreds of family life education Web sites are available on the Internet, allowing individuals and families unprecedented access to family life education information. Evaluation is critical to ensuring the quality of and improving these Web sites; yet, few Web site evaluations have been conducted. We formatively evaluated a new family life…

Ocean Drilling Program: Public Information: Promotional Materials

Science.gov Websites

Learning web site) "From Mountains to Monsoons" interactive CD-ROM and Teacher's Guide (August 1997; JOI Learning web site) "Blast from the Past" poster with classroom activities (August 1997; JOI Learning web site) Slides "The ODP in Film" DVD (JOI Learning web site) B-roll

Library Web Site Administration: A Strategic Planning Model For the Smaller Academic Library

ERIC Educational Resources Information Center

Ryan, Susan M.

2003-01-01

Strategic planning provides a useful structure for creating and implementing library web sites. The planned integration of a library's web site into its mission and objectives ensures that the library's community of users will consider the web site one of the most important information tools the library offers.

Information on infantile colic on the World Wide Web.

PubMed

Bailey, Shana D; D'Auria, Jennifer P; Haushalter, Jamie P

2013-01-01

The purpose of this study was to explore and describe the type and quality of information on infantile colic that a parent might access on the World Wide Web. Two checklists were used to evaluate the quality indicators of 24 Web sites and the colic-specific content. Fifteen health information Web sites met more of the quality parameters than the nine commercial sites. Eight Web sites included information about colic and infant abuse, with six being health information sites. The colic-specific content on 24 Web sites reflected current issues and controversies; however, the completeness of the information in light of current evidence varied among the Web sites. Strategies to avoid complications of parental stress or infant abuse were not commonly found on the Web sites. Pediatric professionals must guide parents to reliable colic resources that also include emotional support and understanding of infant crying. A best evidence guideline for the United States would eliminate confusion and uncertainty about which colic therapies are safe and effective for parents and professionals. Copyright © 2013 National Association of Pediatric Nurse Practitioners. Published by Mosby, Inc. All rights reserved.

Systematic Review of Quality of Patient Information on Phalloplasty in the Internet.

PubMed

Karamitros, Georgios A; Kitsos, Nikolaos A; Sapountzis, Stamatis

2017-12-01

An increasing number of patients, considering aesthetic surgery, use Internet health information as their first source of information. However, the quality of information available in the Internet on phalloplasty is currently unknown. This study aimed to assess the quality of patient information on phalloplasty available in the Internet. The assessment of the Web sites was based on the modified Ensuring Quality Information for Patients (EQIP) instrument (36 items). Three hundred Web sites were identified by the most popular Web search engines. Ninety Web sites were assessed after, duplicates, irrelevant sources and Web sites in other languages rather than English were excluded. Only 16 (18%) Web sites addressed >21 items, and scores tended to be higher for Web sites developed by academic centers and the industry than for Web sites developed by private practicing surgeons. The EQIP score achieved by Web sites ranged between 4 and 29 of the total 36 points, with a median value of 17.5 points (interquartile range, 13-21). The top 5 Web sites with the highest scores were identified. The quality of patient information on phalloplasty in the Internet is substandard, and the existing Web sites present inadequate information. There is a dire need to improve the quality of Internet phalloplasty resources for potential patients who might consider this procedure. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Gullible's Travels.

ERIC Educational Resources Information Center

Block, Marylaine

2002-01-01

Discusses how to teach students to evaluate information they find on the Internet. Highlights include motivation of Web site owners; link-checking; having student create Web pages to help with their evaluation skills of other Web sites; critical thinking skills; and helpful Web sites. (LRW)

Prospective analysis of the quality of Spanish health information web sites after 3 years.

PubMed

Conesa-Fuentes, Maria C; Hernandez-Morante, Juan J

2016-12-01

Although the Internet has become an essential source of health information, our study conducted 3 years ago provided evidence of the low quality of Spanish health web sites. The objective of the present study was to evaluate the quality of Spanish health information web sites now, and to compare these results with those obtained 3 years ago. For the original study, the most visited health information web sites were selected through the PageRank® (Google®) system. The present study evaluated the quality of the same web sites from February to May 2013, using the method developed by Bermúdez-Tamayo et al. and HONCode® criteria. The mean quality of the selected web sites was low and has deteriorated since the previous evaluation, especially in regional health services and institutions' web sites. The quality of private web sites remained broadly similar. Compliance with privacy and update criteria also improved in the intervening period. The results indicate that, even in the case of health web sites, design or appearance is more relevant to developers than quality of information. It is recommended that responsible institutions should increase their efforts to eliminate low-quality health information that may further contribute to health problems.

Bloom syndrome ortholog HIM-6 maintains genomic stability in C. elegans.

PubMed

Grabowski, Melissa M; Svrzikapa, Nenad; Tissenbaum, Heidi A

2005-12-01

Bloom syndrome is caused by mutation of the Bloom helicase (BLM), a member of the RecQ helicase family. Loss of BLM function results in genomic instability that causes a high incidence of cancer. It has been demonstrated that BLM is important for maintaining genomic stability by playing a role in DNA recombination and repair; however, the exact function of BLM is not clearly understood. To determine the mechanism by which BLM controls genomic stability in vivo, we examined the phenotypes caused by mutation of the C. elegans BLM helicase ortholog, HIM-6. We find that the loss of HIM-6 leads to genomic instability as evidenced by an increased number of genomic insertions and deletions, which results in visible random mutant phenotypes. In addition to the mutator phenotype, him-6 mutants have a low brood size, a high incidence of males, a shortened life span, and an increased amount of germ line apoptosis. Upon exposure to high temperature, him-6 mutants that are serially passed become sterile demonstrating a mortal germ line phenotype. Our data suggest a model in which loss of HIM-6 results in genomic instability due to an increased number of DNA lesions, which either cannot be repaired and/or are introduced by low fidelity recombination events. The increased level of genomic instability that leads to him-6(ok412) mutants having a shortened life span.

Roadmap for a Departmental Web Site

ERIC Educational Resources Information Center

Zhang, Guo-Qiang; White, Lee; Hesse, Christopher; Buchner, Marc; Mehregany, Mehran

2005-01-01

Virtually every academic department in an institute of higher education requires Web presence as a critical component of its information technology strategy. The problem of how to leverage the World Wide Web and build effective and useful departmental Web sites seems to have long been solved. Yet browsing academic Web sites from around the world…

The quality of mental health information commonly searched for on the Internet.

PubMed

Grohol, John M; Slimowicz, Joseph; Granda, Rebecca

2014-04-01

Previous research has reviewed the quality of online information related to specific mental disorders. Yet, no comprehensive study has been conducted on the overall quality of mental health information searched for online. This study examined the first 20 search results of two popular search engines-Google and Bing-for 11 common mental health terms. They were analyzed using the DISCERN instrument, an adaptation of the Depression Website Content Checklist (ADWCC), Flesch Reading Ease and Flesch-Kincaid Grade Level readability measures, HONCode badge display, and commercial status, resulting in an analysis of 440 web pages. Quality of Web site results varied based on type of disorder examined, with higher quality Web sites found for schizophrenia, bipolar disorder, and dysthymia, and lower quality ratings for phobia, anxiety, and panic disorder Web sites. Of the total Web sites analyzed, 67.5% had good or better quality content. Nearly one-third of the search results produced Web sites from three entities: WebMD, Wikipedia, and the Mayo Clinic. The mean Flesch Reading Ease score was 41.21, and the mean Flesch-Kincaid Grade Level score was 11.68. The presence of the HONCode badge and noncommercial status was found to have a small correlation with Web site quality, and Web sites displaying the HONCode badge and commercial sites had lower readability scores. Popular search engines appear to offer generally reliable results pointing to mostly good or better quality mental health Web sites. However, additional work is needed to make these sites more readable.

Accredited hand surgery fellowship Web sites: analysis of content and accessibility.

PubMed

Trehan, Samir K; Morrell, Nathan T; Akelman, Edward

2015-04-01

To assess the accessibility and content of accredited hand surgery fellowship Web sites. A list of all accredited hand surgery fellowships was obtained from the online database of the American Society for Surgery of the Hand (ASSH). Fellowship program information on the ASSH Web site was recorded. All fellowship program Web sites were located via Google search. Fellowship program Web sites were analyzed for accessibility and content in 3 domains: program overview, application information/recruitment, and education. At the time of this study, there were 81 accredited hand surgery fellowships with 169 available positions. Thirty of 81 programs (37%) had a functional link on the ASSH online hand surgery fellowship directory; however, Google search identified 78 Web sites. Three programs did not have a Web site. Analysis of content revealed that most Web sites contained contact information, whereas information regarding the anticipated clinical, research, and educational experiences during fellowship was less often present. Furthermore, information regarding past and present fellows, salary, application process/requirements, call responsibilities, and case volume was frequently lacking. Overall, 52 of 81 programs (64%) had the minimal online information required for residents to independently complete the fellowship application process. Hand fellowship program Web sites could be accessed either via the ASSH online directory or Google search, except for 3 programs that did not have Web sites. Although most fellowship program Web sites contained contact information, other content such as application information/recruitment and education, was less frequently present. This study provides comparative data regarding the clinical and educational experiences outlined on hand fellowship program Web sites that are of relevance to residents, fellows, and academic hand surgeons. This study also draws attention to various ways in which the hand surgery fellowship application process can be made more user-friendly and efficient. Copyright © 2015 American Society for Surgery of the Hand. Published by Elsevier Inc. All rights reserved.

Bent Bragg–Laue monochromator for high-energy X-rays

DOE PAGES

Shi, Xianbo; Xu, Wenqian; Yakovenko, Andrey; ...

2017-07-26

A bent Bragg–Laue monochromator (BLM) is proposed for high-energy X-ray (~25–60 keV) beamlines. The BLM has the unique feature of bi-directional focusing. A sagittally bent Laue crystal can focus the large horizontal fan of a bending magnet or wiggler source. A meridionally bent Bragg crystal focuses the beam vertically and corrects for the anticlastic bending effects of the Laue crystal. This monochromator geometry relies on the crystal orientations being optimized. We show that the focusing condition and Rowland condition can be simultaneously satisfied at a given energy. A detailed ray tracings indicate that a BLM can provide similar energy resolutionmore » and higher flux density compared to a sagittally bent double-Laue monochromator configuration. A prototype BLM with a symmetric Bragg crystal and an asymmetric Laue crystal was tested. Matching of the bend radii of the two crystals in the meridional direction was demonstrated. Generally, the horizontal acceptance of the sagittally bent Laue crystal is limited by the large curvature. This horizontal BLM acceptance could be increased by translating the Laue crystal along its sagittal bending axis.« less

Bent Bragg–Laue monochromator for high-energy X-rays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shi, Xianbo; Xu, Wenqian; Yakovenko, Andrey

A bent Bragg–Laue monochromator (BLM) is proposed for high-energy X-ray (~25–60 keV) beamlines. The BLM has the unique feature of bi-directional focusing. A sagittally bent Laue crystal can focus the large horizontal fan of a bending magnet or wiggler source. A meridionally bent Bragg crystal focuses the beam vertically and corrects for the anticlastic bending effects of the Laue crystal. This monochromator geometry relies on the crystal orientations being optimized. We show that the focusing condition and Rowland condition can be simultaneously satisfied at a given energy. A detailed ray tracings indicate that a BLM can provide similar energy resolutionmore » and higher flux density compared to a sagittally bent double-Laue monochromator configuration. A prototype BLM with a symmetric Bragg crystal and an asymmetric Laue crystal was tested. Matching of the bend radii of the two crystals in the meridional direction was demonstrated. Generally, the horizontal acceptance of the sagittally bent Laue crystal is limited by the large curvature. This horizontal BLM acceptance could be increased by translating the Laue crystal along its sagittal bending axis.« less

Public transparency Web sites for radiology practices: prevalence of price, clinical quality, and service quality information.

PubMed

Rosenkrantz, Andrew B; Doshi, Ankur M

2016-01-01

To assess information regarding radiology practices on public transparency Web sites. Eight Web sites comparing radiology centers' price and quality were identified. Web site content was assessed. Six of eight Web sites reported examination prices. Other reported information included hours of operation (4/8), patient satisfaction (2/8), American College of Radiology (ACR) accreditation (3/8), on-site radiologists (2/8), as well as parking, accessibility, waiting area amenities, same/next-day reports, mammography follow-up rates, examination appropriateness, radiation dose, fellowship-trained radiologists, and advanced technologies (1/8 each). Transparency Web sites had a preponderance of price (and to a lesser extent service quality) information, risking fostering price-based competition at the expense of clinical quality. Copyright © 2016 Elsevier Inc. All rights reserved.

Initiatives to Develop Web Sites Including Information about Brownfields Properties

EPA Pesticide Factsheets

This web site was created to assist in planning, designing, and operating web sites that include information about individual brownfields properties. The report is of value to parties designing or managing such sites.

Promoting Your Web Site.

ERIC Educational Resources Information Center

Raeder, Aggi

1997-01-01

Discussion of ways to promote sites on the World Wide Web focuses on how search engines work and how they retrieve and identify sites. Appropriate Web links for submitting new sites and for Internet marketing are included. (LRW)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carnes, E.T.; Truett, D.F.; Truett, L.F.

In the handful of years since the World Wide Web (WWW or Web) came into being, Web sites have developed at an astonishing rate. With the influx of Web pages comes a disparity of site types, including personal homepages, commercial sales sites, and educational data. The variety of sites and the deluge of information contained on the Web exemplify the individual nature of the WWW. Whereas some people argue that it is this eclecticism which gives the Web its charm, we propose that sites which are repositories of technical data would benefit from standardization. This paper proffers a methodology formore » publishing ecological research on the Web. The template we describe uses capabilities of HTML (the HyperText Markup Language) to enhance the value of the traditional scientific paper.« less

Does an Interactive WebCT Site Help Students Learn?

ERIC Educational Resources Information Center

Elicker, Joelle D.; O'Malley, Alison L.; Williams, Christine M.

2008-01-01

We examined whether students with access to a supplemental course Web site enhanced with e-mail, discussion boards, and chat room capability reacted to it more positively than students who used a Web site with the same content but no communication features. Students used the Web sites on a voluntary basis. At the end of the semester, students…

Investigating Web Sites of Faculties of Education: The Case of Turkey

ERIC Educational Resources Information Center

Kutluca, Tamer; Aydin, Serhat; Baki, Adnan

2009-01-01

The purpose of this paper is to explore the current status of the web sites of the Faculties of Education (FOEs) in Turkey. Bearing this in mind, a "Web Site Assessment Form" comprising thirty-seven items was developed and the web sites of the FOEs were evaluated with respect to "Content," "Currency," "Structure…

75 FR 42599 - Posting of Flight Delay Data on Web Sites

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-22

...] RIN No. 2105-AE02 Posting of Flight Delay Data on Web Sites AGENCY: Office of the Secretary (OST... performance information to a reporting air carrier's Web site from anytime between the 20th and 23rd day of... flight performance data onto their Web sites on Saturday, July 24, 2010, for June data, and all...

75 FR 384 - Event Problem Codes Web Site; Center for Devices and Radiological Health; Availability

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-05

...] Event Problem Codes Web Site; Center for Devices and Radiological Health; Availability AGENCY: Food and... the availability of a Web site where the Center for Devices and Radiological Health (CDRH) is posting... to all reporters (Sec. 803.21(b)). FDA is announcing the availability of a Web site that will make...

75 FR 75962 - Proposed Information Collection; Comment Request; Commerce.Gov Web Site User Survey

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-07

...; Commerce.Gov Web Site User Survey AGENCY: Office of the Secretary, Office of Public Affairs. ACTION: Notice... serve users of Commerce.gov and the Department of Commerce bureaus' Web sites, the Offices of Public Affairs will collect information from users about their experience on the Web sites. A random number of...

49 CFR 375.213 - What information must I provide to a prospective individual shipper?

Code of Federal Regulations, 2011 CFR

2011-10-01

... hyperlink on your Internet Web site to the FMCSA Web site containing the information in FMCSA's publication... Internet Web site to the FMCSA Web site containing the information in FMCSA's publication “Your Rights and... explanation that individual shippers may examine these tariff sections or have copies sent to them upon...

76 FR 22926 - Notice of Availability of Draft Environmental Assessment and Finding of No Significant Impact for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-25

... the following methods: Federal Rulemaking Web site: Go to http://www.regulations.gov and search for... writing or in electronic form will be posted on the NRC Web site and on the Federal rulemaking Web site... electronically under ADAMS Accession Number ML110870992. Federal Rulemaking Web site: Public comments and...

How Accessible Are Public Libraries' Web Sites? A Study of Georgia Public Libraries

ERIC Educational Resources Information Center

Ingle, Emma; Green, Ravonne A.; Huprich, Julia

2009-01-01

One issue that public librarians must consider when planning Web site design is accessibility for patrons with disabilities. This article reports a study of Web site accessibility of public libraries in Georgia. The focus of the report is whether public libraries use accessible guidelines and standards in making their Web sites accessible. An…

Streamlining Data for Cross-Platform Web Delivery

ERIC Educational Resources Information Center

Watkins, Sean; Battles, Jason; Vacek, Rachel

2013-01-01

Smartphone users expect the presentation of Web sites on their mobile browsers to look and feel like native applications. With the pressure on library Web developers to produce app-like mobile sites, there is often a rush to get a site up without considering the importance of reusing or even restructuring the data driving the Web sites. An…

Assessing an Infant Feeding Web Site as a Nutrition Education Tool for Child Care Providers

ERIC Educational Resources Information Center

Clark, Alena; Anderson, Jennifer; Adams, Elizabeth; Baker, Susan; Barrett, Karen

2009-01-01

Objective: Determine child care providers' infant feeding knowledge, attitude and behavior changes after viewing the infant feeding Web site and determine the effectiveness of the Web site and bilingual educational materials. Design: Intervention and control groups completed an on-line pretest survey, viewed a Web site for 3 months, and completed…

12 CFR 4.4 - Washington office and web site.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 1 2013-01-01 2013-01-01 false Washington office and web site. 4.4 Section 4.4... EXAMINERS Organization and Functions § 4.4 Washington office and web site. The Washington office of the OCC...'s Web site is at http://www.occ.gov. [76 FR 43561, July 21, 2011] ...

12 CFR 4.4 - Washington office and web site.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 1 2014-01-01 2014-01-01 false Washington office and web site. 4.4 Section 4.4... EXAMINERS Organization and Functions § 4.4 Washington office and web site. The Washington office of the OCC...'s Web site is at http://www.occ.gov. [76 FR 43561, July 21, 2011] ...

12 CFR 4.4 - Washington office and web site.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 1 2012-01-01 2012-01-01 false Washington office and web site. 4.4 Section 4.4... EXAMINERS Organization and Functions § 4.4 Washington office and web site. The Washington office of the OCC...'s Web site is at http://www.occ.gov. [76 FR 43561, July 21, 2011] ...

Food marketing on popular children's web sites: a content analysis.

PubMed

Alvy, Lisa M; Calvert, Sandra L

2008-04-01

In 2006 the Institute of Medicine (IOM) concluded that food marketing was a contributor to childhood obesity in the United States. One recommendation of the IOM committee was for research on newer marketing venues, such as Internet Web sites. The purpose of this cross-sectional study was to answer the IOM's call by examining food marketing on popular children's Web sites. Ten Web sites were selected based on market research conducted by KidSay, which identified favorite sites of children aged 8 to 11 years during February 2005. Using a standardized coding form, these sites were examined page by page for the existence, type, and features of food marketing. Web sites were compared using chi2 analyses. Although food marketing was not pervasive on the majority of the sites, seven of the 10 Web sites contained food marketing. The products marketed were primarily candy, cereal, quick serve restaurants, and snacks. Candystand.com, a food product site, contained a significantly greater amount of food marketing than the other popular children's Web sites. Because the foods marketed to children are not consistent with a healthful diet, nutrition professionals should consider joining advocacy groups to pressure industry to reduce online food marketing directed at youth.

Validation of a biotic ligand model on site-specific copper toxicity to Daphnia magna in the Yeongsan River, Korea.

PubMed

Park, Jinhee; Ra, Jin-Sung; Rho, Hojung; Cho, Jaeweon; Kim, Sang Don

2018-03-01

The objective of this study was to determine whether the water effect ratio (WER) or biotic ligand model (BLM) could be applied to efficiently develop water quality criteria (WQC) in Korea. Samples were collected from 12 specific sites along the Yeongsan River (YSR), Korea, including two sewage treatment plants and one estuary lake. A copper toxicity test using Daphnia magna was performed to determine the WER and to compare to the BLM prediction. The results of the WER from YSR samples also indicated significantly different copper toxicities in all sites. The model-based predictions showed that effluent and estuary waters had significantly different properties in regard to their ability to be used to investigate water characteristics and copper toxicity. It was supposed that the slight water characteristics changes, such as pH, DOC, hardness, conductivity, among others, influence copper toxicity, and these variable effects on copper toxicity interacted with the water composition. The 38% prediction was outside of the validation range by a factor of two in all sites, showing a poor predictive ability, especially in STPs and streams adjacent to the estuary, while the measured toxicity was more stable. The samples that ranged from pH 7.3-7.7 generated stable predictions, while other samples, including those with lower and the higher pH values, led to more unstable predictions. The results also showed that the toxicity of Cu in sample waters to D. magna was closely proportional to the amounts of acidity, including the carboxylic and phenolic groups, as well as the DOC concentrations. Consequently, the acceptable prediction of metal toxicity in various water samples needs the site-specific results considering the water characteristics such as pH and DOC properties particularly in STPs and estuary regions. Copyright © 2017 Elsevier Inc. All rights reserved.

Disclaimer for external Web links | National Oceanic and Atmospheric

Science.gov Websites

Web links The appearance of external links on this Web site does not constitute endorsement by the Department of Commerce/National Oceanic and Atmospheric Administration of external Web sites or the . These links are provided consistent with the stated purpose of this Department of Commerce/NOAA Web site

One Course, One Web Site--Of Course? Maybe Not!

ERIC Educational Resources Information Center

Cohn, Ellen R.

2004-01-01

Colleges and universities increasingly employ commercial Web-based course management systems (such as Blackboard and WebCT). How is it, then, that these institutions unquestioningly allocate a unique Web site to each class? Why establish one Web site for one course when other options provide so many benefits? Why isn't there a clamor for…

COST EFFECTIVE REGULATORY APPROACHES TO ENHANCE DOMESTIC OIL & GAS PRODUCTION AND ENSURE THE PROTECTION OF THE ENVIRONMENT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ben Grunewald; Paul Jehn; Tom Gillespie

The Environmental Information Management Suite/Risk Based Data Management System (EIMS/RBDMS) and Cost Effective Regulatory Approach (CERA) programs continue to be successful. All oil and gas state regulatory programs participate in these efforts. Significant accomplishments include: streamline regulatory approaches, enhancing environmental protection, and making oil and gas data available via the Internet. Oil and gas companies worldwide now have access to data on state web sites. This reduces the cost of exploration and enables companies to develop properties in areas that would have been cost prohibited for exploration. Early in project, GWPC and State Oil and Gas agencies developed the EIMSmore » and CERA strategic plan to prioritize long term development and implementation. The planning process identifies electronic commerce and coal bed methane as high priorities. The group has involved strategic partners in industry and government to develop a common data exchange process. Technical assistance to Alaska continues to improve their program management capabilities. New initiatives in Alaska include the development of an electronic permit tracking system. This system allows managers to expedite the permitting process. Nationwide, the RBDMS system is largely completed with 22 states and one Indian Nation now using this nationally accepted data management system. Additional remaining tasks include routine maintenance and the installation of the program upon request for the remaining oil and gas states. The GWPC in working with the BLM and MMS to develop an XML schema to facilitate electronic permitting and reporting (Appendix A, B, and C). This is a significant effort and, in years to come, will increase access to federal lands by reducing regulatory barriers. The new initiatives are coal bed methane and e-commerce. The e-commerce program will provide industry and BLM/MMS access to the millions of data points housed in the RBDMS system. E-commerce will streamline regulatory approaches and allow small operators to produce energy from areas that have become sub-economic for the major producers. The GWPC is working with states to develop a coal bed methane program, which will both manage the data and develop a public education program on the benefits of produced water. The CERA program benefits all oil and gas states by reducing the cost of regulatory compliance, increasing environmental protection, and providing industry and regulatory agencies a discussion forum. Activities included many small and large group forum settings for discussions of technical and policy issues as well as the ongoing State Class II UIC peer review effort. The accomplishments detailed in this report will be the basis for the next initiative which is RBDMS On-Line. RBDMS On-Line will combine data mining, electronic permitting and electronic reporting with .net technology. Industry, BLM, GWPC and all Oil and Gas states are partnering this effort.« less

Integrating NASA Dryden Research Endeavors into the Teaching-Learning of Mathematics in the K-12 Classroom via the WWW

NASA Technical Reports Server (NTRS)

Ward, Robin A.

2002-01-01

The primary goal of this project was to continue populating the currently existing web site developed in 1998 in conjunction with the NASA Dryden Flight Research Center and California Polytechnic State University, with more mathematics lesson plans and activities that K-12 teachers, students, home-schoolers, and parents could access. All of the activities, while demonstrating some mathematical topic, also showcase the research endeavors of the NASA Dryden Flight Research Center. The website is located at: http://daniel.calpoly.edu/dfrc/Robin. The secondary goal of this project was to share the web-based activities with educators at various conferences and workshops. To address the primary goal of this project, over the past year, several new activities were posted on the web site and some of the existing activities were enhanced to contain more video clips, photos, and materials for teachers. To address the project's secondary goal, the web-based activities were showcased at several conferences and workshops. Additionally, in order to measure and assess the outreach impact of the web site, a link to the web site hitbox.com was established in April 2001, which allowed for the collection of traffic statistics against the web site (such as the domains of visitors, the frequency of visitors to this web site, etc.) Provided is a description of some of the newly created activities posted on the web site during the project period of 2001-2002, followed by a description of the conferences and workshops at which some of the web-based activities were showcased. Next is a brief summary of the web site's traffic statistics demonstrating its worldwide educational impact, followed by a listing of some of the awards and accolades the web site has received.

Setting the renormalization scale in pQCD: Comparisons of the principle of maximum conformality with the sequential extended Brodsky-Lepage-Mackenzie approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ma, Hong -Hao; Wu, Xing -Gang; Ma, Yang

A key problem in making precise perturbative QCD (pQCD) predictions is how to set the renormalization scale of the running coupling unambiguously at each finite order. The elimination of the uncertainty in setting the renormalization scale in pQCD will greatly increase the precision of collider tests of the Standard Model and the sensitivity to new phenomena. Renormalization group invariance requires that predictions for observables must also be independent on the choice of the renormalization scheme. The well-known Brodsky-Lepage-Mackenzie (BLM) approach cannot be easily extended beyond next-to-next-to-leading order of pQCD. Several suggestions have been proposed to extend the BLM approach tomore » all orders. In this paper we discuss two distinct methods. One is based on the “Principle of Maximum Conformality” (PMC), which provides a systematic all-orders method to eliminate the scale and scheme ambiguities of pQCD. The PMC extends the BLM procedure to all orders using renormalization group methods; as an outcome, it significantly improves the pQCD convergence by eliminating renormalon divergences. An alternative method is the “sequential extended BLM” (seBLM) approach, which has been primarily designed to improve the convergence of pQCD series. The seBLM, as originally proposed, introduces auxiliary fields and follows the pattern of the β0-expansion to fix the renormalization scale. However, the seBLM requires a recomputation of pQCD amplitudes including the auxiliary fields; due to the limited availability of calculations using these auxiliary fields, the seBLM has only been applied to a few processes at low orders. In order to avoid the complications of adding extra fields, we propose a modified version of seBLM which allows us to apply this method to higher orders. As a result, we then perform detailed numerical comparisons of the two alternative scale-setting approaches by investigating their predictions for the annihilation cross section ratio R e+e– at four-loop order in pQCD.« less

Direct-to-consumer advertising via the Internet: the role of Web site design.

PubMed

Sewak, Saurabh S; Wilkin, Noel E; Bentley, John P; Smith, Mickey C

2005-06-01

Recent attempts to propose criteria for judging the quality of pharmaceutical and healthcare Web sites do not distinguish between attributes of Web site design related to content and other attributes not related to the content. The Elaboration Likelihood Model from persuasion literature is used as a framework for investigating the effects of Web site design on consequents like attitude and knowledge acquisition. A between-subjects, 2 (high or low involvement)x2 (Web site designed with high or low aspects of visual appeal) factorial design was used in this research. College students were randomly assigned to these treatment groups yielding a balanced design with 29 observations per treatment cell. Analysis of variance results for the effects of involvement and Web site design on attitude and knowledge indicated that the interaction between the independent variables was not significant in both analyses. Examination of main effects revealed that participants who viewed the Web site with higher visual appeal actually had slightly lower knowledge scores (6.32) than those who viewed the Web site with lower visual appeal (7.03, F(1,112)=3.827, P=.053). Results of this research seem to indicate that aspects of Web site design (namely aspects of visual appeal and quality) may not play a role in attaining desired promotional objectives, which can include development of favorable attitudes toward the product and facilitating knowledge acquisition.

Evaluation of Quality, Content, and Use of the Web Site Prepared for Family Members Giving Care to Stroke Patients.

PubMed

Demir, Yasemin; Gozum, Sebahat

2015-09-01

This study was designed to evaluate the quality, content, usability, and efficacy of a Web site prepared for the purpose of improving the caregiving capability of family members who provide care for stroke survivors at home. The DISCERN score for the Web site was found to be 4.35 over 5. The first section that assesses reliability of the Web site was 4.38 over 5; mean score of the second section that measures the quality of the provided information on treatment/care options was 4.30, and mean score of the third section that gives a general evaluation of the material was 4.1. The Web site content achieved an average score of 3.47 over 4 after evaluation by experts. The Web site system usability score was found to be 79.4 over 100. The Web site was utilized mostly for exercises in bed (76.3%; n = 29), use of medications, and patient safety (68.4%; n = 26). It was determined that those who were younger and employed and had no previous experience of nursing any patient utilized relatively more from the section of patient nutrition and oral care and married family caregivers from the body hygiene section. The Web site quality and content were judged to be good and reliable to use. The Web site was efficiently used by caregivers.

A usability evaluation exploring the design of American Nurses Association state web sites.

PubMed

Alexander, Gregory L; Wakefield, Bonnie J; Anbari, Allison B; Lyons, Vanessa; Prentice, Donna; Shepherd, Marilyn; Strecker, E Bradley; Weston, Marla J

2014-08-01

National leaders are calling for opportunities to facilitate the Future of Nursing. Opportunities can be encouraged through state nurses association Web sites, which are part of the American Nurses Association, that are well designed, with appropriate content, and in a language professional nurses understand. The American Nurses Association and constituent state nurses associations provide information about nursing practice, ethics, credentialing, and health on Web sites. We conducted usability evaluations to determine compliance with heuristic and ethical principles for Web site design. We purposefully sampled 27 nursing association Web sites and used 68 heuristic and ethical criteria to perform systematic usability assessments of nurse association Web sites. Web site analysis included seven double experts who were all RNs trained in usability analysis. The extent to which heuristic and ethical criteria were met ranged widely from one state that met 0% of the criteria for "help and documentation" to states that met greater than 92% of criteria for "visibility of system status" and "aesthetic and minimalist design." Suggested improvements are simple yet make an impact on a first-time visitor's impression of the Web site. For example, adding internal navigation and tracking features and providing more details about the application process through help and frequently asked question documentation would facilitate better use. Improved usability will improve effectiveness, efficiency, and consumer satisfaction with these Web sites.

Promoting Teachers' Positive Attitude towards Web Use: A Study in Web Site Development

ERIC Educational Resources Information Center

Akpinar, Yavuz; Bayramoglu, Yusuf

2008-01-01

The purpose of the study was to examine effects of a compact training for developing web sites on teachers' web attitude, as composed of: web self efficacy, perceived web enjoyment, perceived web usefulness and behavioral intention to use the web. To measure the related constructs, the Web Attitude Scale was adapted into Turkish and tested with a…

Assessing Perceived Credibility of Web Sites in a Terrorism Context: The PFLP, Tamil Tigers, Hamas, and Hezbollah

ERIC Educational Resources Information Center

Spinks, Brandon Todd

2009-01-01

The purpose of the study was to contribute to the overall understanding of terrorist organizations' use of the Internet and to increase researchers' knowledge of Web site effectiveness. The methodological approach was evaluation of the perceived credibility of Web sites based on existing criteria derived from information users. The Web sites of…

Beyond Electronic Brochures: An Analysis of Singapore Primary School Web Sites

ERIC Educational Resources Information Center

Hu, Chun; Soong, Andrew Kheng Fah

2007-01-01

This study aims to investigate how Singapore primary schools use their web sites, what kind of information is contained in the web sites, and how the information is presented. Based on an analysis of 176 primary school web sites, which represent all but one of the country's primary schools, findings indicate that most of Singapore's primary school…

76 FR 10072 - Proposed Generic Communications; Draft NRC Regulatory Issue Summary 2011-XX, Adequacy of Station...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-23

... electronic form will be posted on the NRC Web site and on the Federal Rulemaking Web site Regulations.gov... that they do not want publicly disclosed. Federal rulemaking Web site: Go to http://www.regulations.gov... through this Web site. Address questions about NRC dockets to Carol Gallagher, telephone: 301-492-3668, e...

75 FR 34925 - Posting of Flight Delay Data on Web Sites

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-21

...] RIN No. 2105-AE02 Posting of Flight Delay Data on Web Sites AGENCY: Office of the Secretary (OST... carrier's Web site from anytime between the 20th and 23rd day of the month to the fourth Saturday of the... the requirement to post flight delay data on carriers' Web sites. Moreover, this change would further...

Security & Privacy Policy - Naval Oceanography Portal

Science.gov Websites

Notice: This is a U.S. Government Web Site 1. This is a World Wide Web site for official information information on this Web site are strictly prohibited and may be punishable under the Computer Fraud and Abuse Information Act (FOIA) | External Link Disclaimer This is an official U.S. Navy web site. Security &

75 FR 58411 - Medicare Program; Town Hall Meeting on the Physician Compare Web Site, October 27, 2010

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-24

...] Medicare Program; Town Hall Meeting on the Physician Compare Web Site, October 27, 2010 AGENCY: Centers for... establish a Physician Compare Web site by January 1, 2011. This notice announces a Town Hall meeting to discuss the Physician Compare Web site. The purpose of this Town Hall meeting is to solicit input from...

75 FR 32005 - National Emission Standards for Hazardous Air Pollutants for Major Sources: Industrial...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-04

... Web site. E-mail: Comments may be sent by electronic mail (e-mail) to a-and-r[email protected] otherwise protected through http://www.regulations.gov or e-mail. The http://www.regulations.gov Web site is... Web site: http://www.epa.gov/airquality/combustion . Please refer to this Web site to confirm the date...

Food and Beverage Brands that Market to Children and Adolescents on the Internet: A Content Analysis of Branded Web Sites

ERIC Educational Resources Information Center

Henry, Anna E.; Story, Mary

2009-01-01

Objective: To identify food and beverage brand Web sites featuring designated children's areas, assess marketing techniques present on those industry Web sites, and determine nutritional quality of branded food items marketed to children. Design: Systematic content analysis of food and beverage brand Web sites and nutrient analysis of food and…

12 CFR 611.1216 - Public availability of documents related to the termination.

Code of Federal Regulations, 2012 CFR

2012-01-01

... the termination. (a) We may post on our Web site, or require you to post on your Web site: (1) Results... related transactions. (b) We will not post confidential information on our Web site and will not require you to post it on your Web site. (c) You may request that we treat specific information as...

12 CFR 611.1216 - Public availability of documents related to the termination.

Code of Federal Regulations, 2014 CFR

2014-01-01

... the termination. (a) We may post on our Web site, or require you to post on your Web site: (1) Results... related transactions. (b) We will not post confidential information on our Web site and will not require you to post it on your Web site. (c) You may request that we treat specific information as...

12 CFR 611.1216 - Public availability of documents related to the termination.

Code of Federal Regulations, 2013 CFR

2013-01-01

... the termination. (a) We may post on our Web site, or require you to post on your Web site: (1) Results... related transactions. (b) We will not post confidential information on our Web site and will not require you to post it on your Web site. (c) You may request that we treat specific information as...

12 CFR 611.1216 - Public availability of documents related to the termination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... the termination. (a) We may post on our Web site, or require you to post on your Web site: (1) Results... related transactions. (b) We will not post confidential information on our Web site and will not require you to post it on your Web site. (c) You may request that we treat specific information as...

75 FR 11972 - Self-Regulatory Organizations; Financial Industry Regulatory Authority, Inc.; Order Approving...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-12

... Business Member's Web site may not reflect 100 percent of that member's volume for that ATS dark pool...' Web sites to see the total volume for any given ATS dark pool, and the TRF Business Members will make... they receive for each ATS dark pool on their Web site and must prominently disclose that the Web site...

Detecting ecosystem performance anomalies for land management in the upper colorado river basin using satellite observations, climate data, and ecosystem models

USGS Publications Warehouse

Gu, Yingxin; Wylie, B.K.

2010-01-01

This study identifies areas with ecosystem performance anomalies (EPA) within the Upper Colorado River Basin (UCRB) during 2005-2007 using satellite observations, climate data, and ecosystem models. The final EPA maps with 250-m spatial resolution were categorized as normal performance, underperformance, and overperformance (observed performance relative to weather-based predictions) at the 90% level of confidence. The EPA maps were validated using "percentage of bare soil" ground observations. The validation results at locations with comparable site potential showed that regions identified as persistently underperforming (overperforming) tended to have a higher (lower) percentage of bare soil, suggesting that our preliminary EPA maps are reliable and agree with ground-based observations. The 3-year (2005-2007) persistent EPA map from this study provides the first quantitative evaluation of ecosystem performance anomalies within the UCRB and will help the Bureau of Land Management (BLM) identify potentially degraded lands. Results from this study can be used as a prototype by BLM and other land managers for making optimal land management decisions. ?? 2010 by the authors.

Detecting Ecosystem Performance Anomalies for Land Management in the Upper Colorado River Basin Using Satellite Observations, Climate Data, and Ecosystem Models

USGS Publications Warehouse

Gu, Yingxin; Wylie, Bruce K.

2010-01-01

This study identifies areas with ecosystem performance anomalies (EPA) within the Upper Colorado River Basin (UCRB) during 2005–2007 using satellite observations, climate data, and ecosystem models. The final EPA maps with 250-m spatial resolution were categorized as normal performance, underperformance, and overperformance (observed performance relative to weather-based predictions) at the 90% level of confidence. The EPA maps were validated using “percentage of bare soil” ground observations. The validation results at locations with comparable site potential showed that regions identified as persistently underperforming (overperforming) tended to have a higher (lower) percentage of bare soil, suggesting that our preliminary EPA maps are reliable and agree with ground-based observations. The 3-year (2005–2007) persistent EPA map from this study provides the first quantitative evaluation of ecosystem performance anomalies within the UCRB and will help the Bureau of Land Management (BLM) identify potentially degraded lands. Results from this study can be used as a prototype by BLM and other land managers for making optimal land management decisions.

Effectiveness of off-line and web-based promotion of health information web sites.

PubMed

Jones, Craig E; Pinnock, Carole B

2002-01-01

The relative effectiveness of off-line and web-based promotional activities in increasing the use of health information web sites by target audiences were compared. Visitor sessions were classified according to their method of arrival at the site (referral) as external web site, search engine, or "no referrer" (i.e., visitor arriving at the site by inputting URL or using bookmarks). The number of Australian visitor sessions correlated with no referrer referrals but not web site or search-engine referrals. Results showed that the targeted consumer group is more likely to access the web site as a result of off-line promotional activities. The properties of target audiences likely to influence the effectiveness of off-line versus on-line promotional strategies include the size of the Internet using population of the target audience, their proficiency in the use of the Internet, and the increase in effectiveness of off-line promotional activities when applied to locally defined target audiences.

Data resources for range-wide assessment of livestock grazing across the sagebrush biome

USGS Publications Warehouse

Assal, T.J.; Veblen, K.E.; Farinha, M.A.; Aldridge, Cameron L.; Casazza, Michael L.; Pyke, D.A.

2012-01-01

The data contained in this series were compiled, modified, and analyzed for the U.S. Geological Survey (USGS) report "Range-Wide Assessment of Livestock Grazing Across the Sagebrush Biome." This report can be accessed through the USGS Publications Warehouse (online linkage: http://pubs.usgs.gov/of/2011/1263/). The dataset contains spatial and tabular data related to Bureau of Land Management (BLM) Grazing Allotments. We reviewed the BLM national grazing allotment spatial dataset available from the GeoCommunicator National Integrated Land System (NILS) website in 2007 (http://www.geocommunicator.gov). We identified several limitations in those data and learned that some BLM State and/or field offices had updated their spatial data to rectify these limitations, but maintained the data outside of NILS. We contacted appropriate BLM offices (State or field, 25 in all) to obtain the most recent data, assessed the data, established a data development protocol, and compiled data into a topologically enforced dataset throughout the area of interest for this project (that is, the pre-settlement distribution of Greater Sage-Grouse in the Western United States). The final database includes three spatial datasets: Allotments (BLM Grazing Allotments), OUT_Polygons (nonallotment polygons used to ensure topology), and Duplicate_Polygon_Allotments. See Appendix 1 of the aforementioned report for complete methods. The tabular data presented here consists of information synthesized by the Land Health Standard (LHS) analysis (Appendix 2), and data obtained from the BLM Rangeland Administration System (http://www.blm.gov/ras/). In 2008, available LHS data for all allotments in all regions were compiled by BLM in response to a Freedom of Information Act (FOIA) request made by a private organization. The BLM provided us with a copy of these data. These data provided three major types of information that were of interest: (1) date(s) (if any) of the most recent LHS evaluation for each allotment; (2) whether if evaluated, each region-specific standard (3–8 LHS depending on region) had been met on a given allotment; and (3) whether livestock contributed to any of these standards not being met. A description of how we processed the original data to prepare for analysis is described in Appendix 2, and the synthesized dataset can be found in the table "lhs_x_walk." Permitted use dates, livestock type (horse, sheep or cattle), number of livestock, and Animal Unit Months [the number of animal units (1,000-pound animal equivalents) that can be grazed for 31 days with the available forage in a sustainable manner] are the legal maximum grazing amounts for a given allotment, and legal adjustments to these numbers occur infrequently. We summarized permitted use by BLM allotment in the table "Permitted_Use." Billed use records are used for calculations of permittees' annual grazing bills. We summarized billed use by allotment for BLM grazing year in the table "Billed_Use." All three tables can be joined with the allotment spatial data in a geographic information system (GIS) environment, using the IDENT attribute as the primary key.

Information about liver transplantation on the World Wide Web.

PubMed

Hanif, F; Sivaprakasam, R; Butler, A; Huguet, E; Pettigrew, G J; Michael, E D A; Praseedom, R K; Jamieson, N V; Bradley, J A; Gibbs, P

2006-09-01

Orthotopic liver transplant (OLTx) has evolved to a successful surgical management for end-stage liver diseases. Awareness and information about OLTx is an important tool in assisting OLTx recipients and people supporting them, including non-transplant clinicians. The study aimed to investigate the nature and quality of liver transplant-related patient information on the World Wide Web. Four common search engines were used to explore the Internet by using the key words 'Liver transplant'. The URL (unique resource locator) of the top 50 returns was chosen as it was judged unlikely that the average user would search beyond the first 50 sites returned by a given search. Each Web site was assessed on the following categories: origin, language, accessibility and extent of the information. A weighted Information Score (IS) was created to assess the quality of clinical and educational value of each Web site and was scored independently by three transplant clinicians. The Internet search performed with the aid of the four search engines yielded a total of 2,255,244 Web sites. Of the 200 possible sites, only 58 Web sites were assessed because of repetition of the same Web sites and non-accessible links. The overall median weighted IS was 22 (IQR 1 - 42). Of the 58 Web sites analysed, 45 (77%) belonged to USA, six (10%) were European, and seven (12%) were from the rest of the world. The median weighted IS of publications originating from Europe and USA was 40 (IQR = 22 - 60) and 23 (IQR = 6 - 38), respectively. Although European Web sites produced a higher weighted IS [40 (IQR = 22 - 60)] as compared with the USA publications [23 (IQR = 6 - 38)], this was not statistically significant (p = 0.07). Web sites belonging to the academic institutions and the professional organizations scored significantly higher with a median weighted IS of 28 (IQR = 16 - 44) and 24(12 - 35), respectively, as compared with the commercial Web sites (median = 6 with IQR of 0 - 14, p = .001). There was an Intraclass Correlation Coefficient (ICC) of 0.89 and an associated 95% CI (0.83, 0.93) for the three observers on the 58 Web sites. The study highlights the need for a significant improvement in the information available on the World Wide Web about OLTx. It concludes that the educational material currently available on the World Wide Web about liver transplant is of poor quality and requires rigorous input from health care professionals. The authors suggest that clinicians should pay more attention to take the necessary steps to improve the standard of information available on their relevant Web sites and must take an active role in helping their patients find Web sites that provide the best and accurate information specifically applicable to the loco-regional circumstances.

76 FR 48919 - NRC Enforcement Policy

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-09

... Web site and on the Federal rulemaking Web site, http://www.regulations.gov . Because your comments... publicly disclosed. You may submit comments by any one of the following methods: Federal Rulemaking Web... Web site: Public comments and supporting materials related to this notice can be found at http://www...

Creating Patient and Family Education Web Sites

PubMed Central

YADRICH, DONNA MACAN; FITZGERALD, SHARON A.; WERKOWITCH, MARILYN; SMITH, CAROL E.

2013-01-01

This article gives details about the methods and processes used to ensure that usability and accessibility were achieved during development of the Home Parenteral Nutrition Family Caregivers Web site, an evidence-based health education Web site for the family members and caregivers of chronically ill patients. This article addresses comprehensive definitions of usability and accessibility and illustrates Web site development according to Section 508 standards and the national Health and Human Services’ Research-Based Web Design and Usability Guidelines requirements. PMID:22024970

Transportation and the Bureau of Land Management

DOT National Transportation Integrated Search

2008-01-01

The Volpe Center prepared a promotional booklet featuring an overview of the BLM's transportation infrastructure. The booklet describes the BLM's roads, bridges, and trails; highlights their economic and recreational importance; and lists key transpo...

Nonlinear Aeroelastic Study for Folding Wing Structures

DTIC Science & Technology

2010-03-29

Blm +B nl m ) ζ (14) δǫm = ( Blm +B nl m ) δζ (15) χ = Bbζ (16) δχ = Bbδζ . (17) Substitution of Eqs. 13-17 into Eq. 11 gives: δζT ( h...S ΨTFdS + ∫ S ΨTΦtrdS − h ∫ S ΨTΨρdSζ̈ − h ∫ S ΨT cΨdSζ̇ − ∫ S ( Blm ) TNdS − ∫ S (Bnlm) TNdS − ∫ S BTb MdS ) = 0 . (18) Considering the arbitrariness...of the virtual generalized displacements, Eq. 18 can be written 6 of 24 as: h ∫ S ΨTΨρdSζ̈ + h ∫ S ΨT cΨdSζ̇ + ∫ S ( Blm ) TNdS + ∫ S (Bnlm) TNdS+ ∫

Nonlinear High Fidelity Aeroelastic Analysis for Novel Configurations

DTIC Science & Technology

2010-03-29

Blm +B nl m ) ζ (14) δǫm = ( Blm +B nl m ) δζ (15) χ = Bbζ (16) δχ = Bbδζ . (17) Substitution of Eqs. 13-17 into Eq. 11 gives: δζT ( h...S ΨTFdS + ∫ S ΨTΦtrdS − h ∫ S ΨTΨρdSζ̈ − h ∫ S ΨT cΨdSζ̇ − ∫ S ( Blm ) TNdS − ∫ S (Bnlm) TNdS − ∫ S BTb MdS ) = 0 . (18) Considering the arbitrariness...of the virtual generalized displacements, Eq. 18 can be written 6 of 24 as: h ∫ S ΨTΨρdSζ̈ + h ∫ S ΨT cΨdSζ̇ + ∫ S ( Blm ) TNdS + ∫ S (Bnlm) TNdS+ ∫

Gas desorption and adsorption isotherm studies of coals in the Powder River basin, Wyoming and adjacent basins in Wyoming and North Dakota

USGS Publications Warehouse

Stricker, Gary D.; Flores, Romeo M.; McGarry, Dwain E.; Stillwell, Dean P.; Hoppe, Daniel J.; Stillwell, Cathy R.; Ochs, Alan M.; Ellis, Margaret S.; Osvald, Karl S.; Taylor, Sharon L.; Thorvaldson, Marjorie C.; Trippi, Michael H.; Grose, Sherry D.; Crockett, Fred J.; Shariff, Asghar J.

2006-01-01

The U.S. Geological Survey (USGS), in cooperation with the State Office, Reservoir Management Group (RMG), of the Bureau of Land Management (BLM) in Casper (Wyoming), investigated the coalbed methane resources (CBM) in the Powder River Basin, Wyoming and Montana, from 1999 to the present. Beginning in late 1999, the study also included the Williston Basin in Montana and North and South Dakota and Green River Basin and Big Horn Basin in Wyoming. The rapid development of CBM (referred to as coalbed natural gas by the BLM) during the early 1990s, and the lack of sufficient data for the BLM to fully assess and manage the resource in the Powder River Basin, in particular, gave impetus to the cooperative program. An integral part of the joint USGS-BLM project was the participation of 25 gas operators that entered individually into confidential agreements with the USGS, and whose cooperation was essential to the study. The arrangements were for the gas operators to drill and core coal-bed reservoirs at their cost, and for the USGS and BLM personnel to then desorb, analyze, and interpret the coal data with joint funding by the two agencies. Upon completion of analyses by the USGS, the data were to be shared with both the BLM and the gas operator that supplied the core, and then to be released or published 1 yr after the report was submitted to the operator.

Stage-specific apoptosis, developmental delay, and embryonic lethality in mice homozygous for a targeted disruption in the murine Bloom's syndrome gene.

PubMed

Chester, N; Kuo, F; Kozak, C; O'Hara, C D; Leder, P

1998-11-01

Bloom's syndrome is a human autosomal genetic disorder characterized at the cellular level by genome instability and increased sister chomatid exchanges (SCEs). Clinical features of the disease include proportional dwarfism and a predisposition to develop a wide variety of malignancies. The human BLM gene has been cloned recently and encodes a DNA helicase. Mouse embryos homozygous for a targeted mutation in the murine Bloom's syndrome gene (Blm) are developmentally delayed and die by embryonic day 13.5. The fact that the interrupted gene is the homolog of the human BLM gene was confirmed by its homologous sequence, its chromosomal location, and by demonstrating high numbers of SCEs in cultured murine Blm-/- fibroblasts. The proportional dwarfism seen in the human is consistent with the small size and developmental delay (12-24 hr) seen during mid-gestation in murine Blm-/- embryos. Interestingly, the growth retardation in mutant embryos can be accounted for by a wave of increased apoptosis in the epiblast restricted to early post-implantation embryogenesis. Mutant embryos do not survive past day 13.5, and at this time exhibit severe anemia. Red blood cells and their precursors from Blm-/- embryos are heterogeneous in appearance and have increased numbers of macrocytes and micronuclei. Both the apoptotic wave and the appearance of micronuclei in red blood cells are likely cellular consequences of damaged DNA caused by effects on replicating or segregating chromosomes.

The C-terminal domain of the Bloom syndrome DNA helicase is essential for genomic stability

PubMed Central

Yankiwski, Victor; Noonan, James P; Neff, Norma F

2001-01-01

Background Bloom syndrome is a rare cancer-prone disorder in which the cells of affected persons have a high frequency of somatic mutation and genomic instability. Bloom syndrome cells have a distinctive high frequency of sister chromatid exchange and quadriradial formation. BLM, the protein altered in BS, is a member of the RecQ DNA helicase family, whose members share an average of 40% identity in the helicase domain and have divergent N-terminal and C-terminal flanking regions of variable lengths. The BLM DNA helicase has been shown to localize to the ND10 (nuclear domain 10) or PML (promyelocytic leukemia) nuclear bodies, where it associates with TOPIIIα, and to the nucleolus. Results This report demonstrates that the N-terminal domain of BLM is responsible for localization of the protein to the nuclear bodies, while the C-terminal domain directs the protein to the nucleolus. Deletions of the N-terminal domain of BLM have little effect on sister chromatid exchange frequency and chromosome stability as compared to helicase and C-terminal mutations which can increase SCE frequency and chromosome abnormalities. Conclusion The helicase activity and the C-terminal domain of BLM are critical for maintaining genomic stability as measured by the sister chromatid exchange assay. The localization of BLM into the nucleolus by the C-terminal domain appears to be more important to genomic stability than localization in the nuclear bodies. PMID:11472631

The FANC pathway and BLM collaborate during mitosis to prevent micro-nucleation and chromosome abnormalities.

PubMed

Naim, Valeria; Rosselli, Filippo

2009-06-01

Loss-of-function of caretaker genes characterizes a group of cancer predisposition diseases that feature cellular hypersensitivity to DNA damage and chromosome fragility; this group includes Fanconi anaemia and Bloom syndrome. The products of the 13 FANC genes (mutated in Fanconi anaemia), which constitute the 'FANC' pathway, and BLM (the RecQ helicase mutated in Bloom syndrome) are thought to collaborate during the S phase of the cell cycle, preventing chromosome instability. Recently, BLM has been implicated in the completion of sister chromatid separation during mitosis, a complex process in which precise regulation and execution is crucial to preserve genomic stability. Here we show for the first time a role for the FANC pathway in chromosome segregation during mitotic cell division. FANCD2, a key component of the pathway, localizes to discrete spots on mitotic chromosomes. FANCD2 chromosomal localization is responsive to replicative stress and specifically targets aphidicolin (APH)-induced chromatid gaps and breaks. Our data indicate that the FANC pathway is involved in rescuing abnormal anaphase and telophase (ana-telophase) cells, limiting aneuploidy and reducing chromosome instability in daughter cells. We further address a cooperative role for the FANC pathway and BLM in preventing micronucleation, through FANC-dependent targeting of BLM to non-centromeric abnormal structures induced by replicative stress. We reveal new crosstalk between FANC and BLM proteins, extending their interaction beyond the S-phase rescue of damaged DNA to the safeguarding of chromosome stability during mitosis.

Surfing for scoliosis: the quality of information available on the Internet.

PubMed

Mathur, Sameer; Shanti, Nael; Brkaric, Mario; Sood, Vivek; Kubeck, Justin; Paulino, Carl; Merola, Andrew A

2005-12-01

A cross section of Web sites accessible to the general public was surveyed. To evaluate the quality and accuracy of information on scoliosis that a patient might access on the Internet. The Internet is a rapidly expanding communications network with an estimated 765 million users worldwide by the year 2005. Medical information is one of the most common sources of inquires on the Web. More than 100 million Americans accessed the Internet for medical information in the year 2000. Undoubtedly, the use of the Internet for patient information needs will continue to expand as Internet access becomes more readily available. This expansion combined with the Internet's poorly regulated format can lead to problems in the quality of information available. Since the Internet operates on a global scale, implementing and enforcing standards have been difficult. The largely uncontrolled information can potentially negatively influence consumer health outcomes. To identify potential sites, five search engines were selected and the word "scoliosis" was entered into each search engine. A total of 50 Web sites were chosen for review. Each Web site was evaluated according to the type of Web site, quality content, and informational accuracy by three board-certified academic orthopedic surgeons, fellowship trained in spinal surgery, who each has been in practice for a minimum of 8 years. Each Web site was categorized as academic, commercial, physician, nonphysician health professional, and unidentified. In addition, each Web site was evaluated according to scoliosis-specific content using a point value system of 32 disease-specific key words pertinent to the care of scoliosis on an ordinal scale. A list of these words is given. Point values were given for the use of key words related to disease summary, classifications, treatment options, and complications. The accuracy of the individual Web site was evaluated by each spine surgeon using a scale of 1 to 4. A score of 1 represents that the examiner agreed with less than 25% of the information while a score of 4 represents greater than 75% agreement. Of the total 50 Web sites evaluated, 44% were academic, 18% were physician based, 16% were commercial, 12% were unidentified, and 10% were nonphysician health professionals. The quality content score (maximum, 32 points) for academic sites was 12.6 +/- 3.8, physician sites 11.3 +/- 4.0, commercial sites 11 +/- 4.2, unidentified 7.6 +/- 3.9, and nonphysician health professional site 7.0 +/- 1.8. The accuracy score (maximum, 12 points) was 6.6 +/- 2.4 for academic sites, 6.3 +/- 3.0 for physician-professional sites, 6.0 +/- 2.7 for unidentified sites, 5.5 +/- 3.8 for nonphysician professional sites, and 5.0 +/- 1.5 for commercial Web sites. The academic Web sites had the highest mean scores in both quality and accuracy content scores. The information about scoliosis on the Internet is of limited quality and poor information value. Although the majority of the Web sites were academic, the content quality and accuracy scores were still poor. The lowest scoring Web sites were the nonphysician professionals and the unidentified sites, which were often message boards. Overall, the highest scoring Web site related to both quality and accuracy of information was www.srs.org. This Web site was designed by the Scoliosis Research Society. The public and the medical communities need to be aware of these existing limitations of the Internet. Based on our review, the physician must assume primary responsibility of educating and counseling their patients.

e-Ana and e-Mia: A Content Analysis of Pro–Eating Disorder Web Sites

PubMed Central

Schenk, Summer; Wilson, Jenny L.; Peebles, Rebecka

2010-01-01

Objectives. The Internet offers Web sites that describe, endorse, and support eating disorders. We examined the features of pro–eating disorder Web sites and the messages to which users may be exposed. Methods. We conducted a systematic content analysis of 180 active Web sites, noting site logistics, site accessories, “thinspiration” material (images and prose intended to inspire weight loss), tips and tricks, recovery, themes, and perceived harm. Results. Practically all (91%) of the Web sites were open to the public, and most (79%) had interactive features. A large majority (84%) offered pro-anorexia content, and 64% provided pro-bulimia content. Few sites focused on eating disorders as a lifestyle choice. Thinspiration material appeared on 85% of the sites, and 83% provided overt suggestions on how to engage in eating-disordered behaviors. Thirty-eight percent of the sites included recovery-oriented information or links. Common themes were success, control, perfection, and solidarity. Conclusions. Pro–eating disorder Web sites present graphic material to encourage, support, and motivate site users to continue their efforts with anorexia and bulimia. Continued monitoring will offer a valuable foundation to build a better understanding of the effects of these sites on their users. PMID:20558807

A GIS-Interface Web Site: Exploratory Learning for Geography Curriculum

ERIC Educational Resources Information Center

Huang, Kuo Hung

2011-01-01

Although Web-based instruction provides learners with sufficient resources for self-paced learning, previous studies have confirmed that browsing navigation-oriented Web sites possibly hampers users' comprehension of information. Web sites designed as "categories of materials" for navigation demand more cognitive effort from users to orient their…

Digital Discernment: An E-Commerce Web Site Evaluation Tool

ERIC Educational Resources Information Center

Sigman, Betsy Page; Boston, Brian J.

2013-01-01

Students entering the business workforce today may well share some responsibility for developing, revising, or evaluating their company's Web site. They may lack the experience, however, to critique their employer's Web presence effectively. The purpose of developing Digital Discernment, an e-commerce Web site evaluation tool, was to prepare…

Shakespeare Goes Online: Web Resources for Teaching Shakespeare.

ERIC Educational Resources Information Center

Schuetz, Carol L.

This annotated bibliography contains five sections and 62 items. The first section lists general resources including six Web site addresses; the second section, on Shakespeare's works, contains five Web site addresses; the third section, on Shakespeare and the Globe Theatre, provides five Web site addresses; the fourth section presents classroom…

E-Marketing: Are Community Colleges Embracing the Web?

ERIC Educational Resources Information Center

Clagett, Craig

2001-01-01

Conducted a pilot survey of community colleges to assess their online marketing efforts. Found that while all had Web sites, only a minority of sites were truly interactive. Involvement of marketing offices with Web sites varied considerably, and a minority had used e-mail or Web ads for marketing. (EV)

Information Architecture for Bilingual Web Sites.

ERIC Educational Resources Information Center

Cunliffe, Daniel; Jones, Helen; Jarvis, Melanie; Egan, Kevin; Huws, Rhian; Munro, Sian

2002-01-01

Discusses creating an information architecture for a bilingual Web site and reports work in progress on the development of a content-based bilingual Web site to facilitate shared resources between speech and language therapists. Considers a structural analysis of existing bilingual Web designs and explains a card-sorting activity conducted with…

Formal Features of Cyberspace: Relationships between Web Page Complexity and Site Traffic.

ERIC Educational Resources Information Center

Bucy, Erik P.; Lang, Annie; Potter, Robert F.; Grabe, Maria Elizabeth

1999-01-01

Examines differences between the formal features of commercial versus noncommercial Web sites, and the relationship between Web page complexity and amount of traffic a site receives. Findings indicate that, although most pages in this stage of the Web's development remain technologically simple and noninteractive, there are significant…

12 CFR 155.310 - How do I notify the OCC?

Code of Federal Regulations, 2014 CFR

2014-01-01

... least 30 days before you establish a transactional Web site. The notice must do three things: (a) Describe the transactional web site. (b) Indicate the date the transactional web site will become operational. (c) List a contact familiar with the deployment, operation, and security of the transactional web...

12 CFR 390.222 - How do I notify FDIC?

Code of Federal Regulations, 2013 CFR

2013-01-01

... 30 days before you establish a transactional Web site. The notice must do three things: (1) Describe the transactional Web site. (2) Indicate the date the transactional Web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional Web...

12 CFR 390.222 - How do I notify FDIC?

Code of Federal Regulations, 2012 CFR

2012-01-01

... 30 days before you establish a transactional Web site. The notice must do three things: (1) Describe the transactional Web site. (2) Indicate the date the transactional Web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional Web...

12 CFR 155.310 - How do I notify the OCC?

Code of Federal Regulations, 2013 CFR

2013-01-01

... least 30 days before you establish a transactional Web site. The notice must do three things: (a) Describe the transactional web site. (b) Indicate the date the transactional web site will become operational. (c) List a contact familiar with the deployment, operation, and security of the transactional web...

12 CFR 390.222 - How do I notify FDIC?

Code of Federal Regulations, 2014 CFR

2014-01-01

... 30 days before you establish a transactional Web site. The notice must do three things: (1) Describe the transactional Web site. (2) Indicate the date the transactional Web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional Web...

12 CFR 155.310 - How do I notify the OCC?

Code of Federal Regulations, 2012 CFR

2012-01-01

... least 30 days before you establish a transactional Web site. The notice must do three things: (a) Describe the transactional web site. (b) Indicate the date the transactional web site will become operational. (c) List a contact familiar with the deployment, operation, and security of the transactional web...

78 FR 66746 - Medical Device User Fee and Modernization Act; Notice to Public of Web Site Location of Fiscal...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-06

...] Medical Device User Fee and Modernization Act; Notice to Public of Web Site Location of Fiscal Year 2014... and Drug Administration (FDA or the Agency) is announcing the Web site location where the Agency will... documents, FDA has committed to updating its Web site in a timely manner to reflect the Agency's review of...

Conceptual Web Users' Actions Prediction for Ontology-Based Browsing Recommendations

NASA Astrophysics Data System (ADS)

Robal, Tarmo; Kalja, Ahto

The Internet consists of thousands of web sites with different kinds of structures. However, users are browsing the web according to their informational expectations towards the web site searched, having an implicit conceptual model of the domain in their minds. Nevertheless, people tend to repeat themselves and have partially shared conceptual views while surfing the web, finding some areas of web sites more interesting than others. Herein, we take advantage of the latter and provide a model and a study on predicting users' actions based on the web ontology concepts and their relations.

Does your web site draw new patients?

PubMed

Wallin, Wendy S

2009-11-01

The absence of scientific data forces orthodontists to guess at how best to design Internet sites that persuade prospective patients to call for appointments. This study was conducted to identify the Web-site factors that lead prospective patients to make appointments or, conversely, to reject a practice. Ten participants actively looking online for an orthodontist were recruited to participate. They reviewed 64 orthodontic Web sites in their geographic areas and rated their likelihood of calling each practice for an appointment. The sessions were videotaped. Analysis of participant comments, navigation patterns, and ratings suggested 25 distinguishing factors. Statistical analysis showed 10 Web-site characteristics that predict the success of an orthodontic Web site in attracting new patients.

ACHP | Federal Historic Preservation Web Sites

Science.gov Websites

Historic Preservation Web Sites Federal Historic Preservation Web Sites Historic American Buildings Survey /Historic American Engineering Record/Historic American Landscapes Survey lcweb2.loc.gov/ammem/hhhtml

[Study on the hepatocytic cell targetability of liposomes].

PubMed

Hou, Xin-pu; Wang, Li; Wang, Xiang-tao; Li, Sha

2003-02-01

To target for hepatocytic cell, liposomes was modified by special ligand. Sterically stabilized liposomes (SSL) was conjugated with asialofeticin (AF), the ligand of asialoglycoprotein receptor (ASGP-R) of hepatocyte. ASGP-R-BLM is the ASGP-R reconstructed on bilayer lipid membrane (BLM). The recognition reaction between AF-SSL and ASGP-R-BLM can be monitored by the varieties of membrane electrical parameters. The targetability of AF-SSL mediated to hepatocyte was detected by radioisotopic labeled in vitro and in vivo. The therapeutic effect of antihepatocarcinoma was observed also. The lifetime of ASGP-R-BLM decreased with the added amount of AF-SSL. It was demonstrated that there was recognition reaction between AF-SSL and ASGP-R-BLM. The combination of AF-SSL with hepatocyte was significantly higher than that of SSL without AF-modified in vitro and in vivo. The survival time of rat for AF-SSL carriered ADM (adriamycin) group was much longer and the toxicities on heart, kidney and lung were lower than those SSL carried ADM group. It is possible to actively target the cell with specific receptor by ligand modified liposomes. The result prvide scientific basis of hepatocyte targeted liposomes.

Not Your Father's Web Site: Corporate Sites Emerge as New Content Innovators.

ERIC Educational Resources Information Center

O'Leary, Mick

2002-01-01

New economy corporate Web sites have pioneered exciting techniques-rich media, interactivity, personalization, community, and integration of much third-party content. Discusses business-to-business (B2B) Web commerce, with examples of several B2B corporate sites; portal and content elements of these sites; and corporate content outlooks. (AEF)

Informational content of official pharmaceutical industry web sites about treatments for erectile dysfunction.

PubMed

Waack, Katherine E; Ernst, Michael E; Graber, Mark A

2004-12-01

In the last 5 years, several treatments have become available for erectile dysfunction (ED). During this same period, consumer use of the Internet for health information has increased rapidly. In traditional direct-to-consumer advertisements, viewers are often referred to a pharmaceutical company Web site for further information. To evaluate the accessibility and informational content of 5 pharmaceutical company Web sites about ED treatments. Using 10 popular search engines and 1 specialized search engine, the accessibility of the official pharmaceutical company-sponsored Web site was determined by searching under brand and generic names. One company also manufactures an ED device; this site was also included. A structured, explicit review of information found on these sites was conducted. Of 110 searches (1 for each treatment, including corresponding generic drug name, using each search engine), 68 yielded the official pharmaceutical company Web site within the first 10 links. Removal of outliers (for both brand and generic name searches) resulted in 68 of 77 searches producing the pharmaceutical company Web site for the brand-name drug in the top 10 links. Although all pharmaceutical company Web sites contained general information on adverse effects and contraindications to use, only 2 sites gave actual percentages. Three sites provided references for their materials or discussed other treatment or drug options, while 4 of the sites contained profound advertising or emotive content. None mentioned cost of the therapy. The information contained on pharmaceutical company Web sites for ED treatments is superficial and aimed primarily at consumers. It is largely promotional and provides only limited information needed to effectively compare treatment options.

Sentiment Analysis of Web Sites Related to Vaginal Mesh Use in Pelvic Reconstructive Surgery.

PubMed

Hobson, Deslyn T G; Meriwether, Kate V; Francis, Sean L; Kinman, Casey L; Stewart, J Ryan

2018-05-02

The purpose of this study was to utilize sentiment analysis to describe online opinions toward vaginal mesh. We hypothesized that sentiment in legal Web sites would be more negative than that in medical and reference Web sites. We generated a list of relevant key words related to vaginal mesh and searched Web sites using the Google search engine. Each unique uniform resource locator (URL) was sorted into 1 of 6 categories: "medical", "legal", "news/media", "patient generated", "reference", or "unrelated". Sentiment of relevant Web sites, the primary outcome, was scored on a scale of -1 to +1, and mean sentiment was compared across all categories using 1-way analysis of variance. Tukey test evaluated differences between category pairs. Google searches of 464 unique key words resulted in 11,405 URLs. Sentiment analysis was performed on 8029 relevant URLs (3472 legal, 1625 "medical", 1774 "reference", 666 "news media", 492 "patient generated"). The mean sentiment for all relevant Web sites was +0.01 ± 0.16; analysis of variance revealed significant differences between categories (P < 0.001). Web sites categorized as "legal" and "news/media" had a slightly negative mean sentiment, whereas those categorized as "medical," "reference," and "patient generated" had slightly positive mean sentiments. Tukey test showed differences between all category pairs except the "medical" versus "reference" in comparison with the largest mean difference (-0.13) seen in the "legal" versus "reference" comparison. Web sites related to vaginal mesh have an overall mean neutral sentiment, and Web sites categorized as "medical," "reference," and "patient generated" have significantly higher sentiment scores than related Web sites in "legal" and "news/media" categories.

H.E.A.L.T.H.: Efficacy of an Internet/Population-Based Behavioral Weight Management Program for the U.S. Army

PubMed Central

Stewart, Tiffany; Han, Hongmei; Allen, H. Raymond; Bathalon, COL Gaston; Ryan, Donna H.; Newton, Robert L.; Williamson, Donald A.

2011-01-01

Background A significant number of soldiers exceed the maximum allowable weight standards or have body weights approaching the maximum allowable weight standards. This mandates development of scalable approaches to improve compliance with military weight standards. Methods We developed an intervention that included two components: (1) an Internet-based weight management program (Web site) and (2) a promotion program designed to promote and sustain usage of the Web site. The Web site remained online for 37 months, with the Web site promotion program ending after 25 months. Results Soldiers’ demographics were as follows: mean age, 32 years; body mass index (BMI), 28 kg/m2; 31% female; and 58% Caucasian. Civilian demographics were as follows: mean age, 38 years; BMI, 30 kg/m2; 84% female; and 55% Caucasian. Results indicated that 2417 soldiers and 2147 civilians (N = 4564) registered on the Web site. In the first 25 months (phase 1) of the study, new participants enrolled on the Web site at a rate of 88 (soldiers) and 80 (civilians) per month. After the promotion program was removed (phase 2), new participants enrolled at a rate of 18 (soldiers) and 13 (civilians) per month. Utilization of the Web site was associated with self-reported weight loss (p < .0001). Participants who utilized the Web site more frequently lost more weight (p < .0001). Participants reported satisfaction with the Web site. Conclusions The Web site and accompanying promotion program, when implemented at a military base, received satisfactory ratings and benefited a subset of participants in promoting weight loss. This justifies further examination of effectiveness in a randomized trial setting. PMID:21303642

Hand Society and Matching Program Web Sites Provide Poor Access to Information Regarding Hand Surgery Fellowship.

PubMed

Hinds, Richard M; Klifto, Christopher S; Naik, Amish A; Sapienza, Anthony; Capo, John T

2016-08-01

The Internet is a common resource for applicants of hand surgery fellowships, however, the quality and accessibility of fellowship online information is unknown. The objectives of this study were to evaluate the accessibility of hand surgery fellowship Web sites and to assess the quality of information provided via program Web sites. Hand fellowship Web site accessibility was evaluated by reviewing the American Society for Surgery of the Hand (ASSH) on November 16, 2014 and the National Resident Matching Program (NRMP) fellowship directories on February 12, 2015, and performing an independent Google search on November 25, 2014. Accessible Web sites were then assessed for quality of the presented information. A total of 81 programs were identified with the ASSH directory featuring direct links to 32% of program Web sites and the NRMP directory directly linking to 0%. A Google search yielded direct links to 86% of program Web sites. The quality of presented information varied greatly among the 72 accessible Web sites. Program description (100%), fellowship application requirements (97%), program contact email address (85%), and research requirements (75%) were the most commonly presented components of fellowship information. Hand fellowship program Web sites can be accessed from the ASSH directory and, to a lesser extent, the NRMP directory. However, a Google search is the most reliable method to access online fellowship information. Of assessable programs, all featured a program description though the quality of the remaining information was variable. Hand surgery fellowship applicants may face some difficulties when attempting to gather program information online. Future efforts should focus on improving the accessibility and content quality on hand surgery fellowship program Web sites.

Differences in the quality of information on the internet about lung cancer between the United States and Japan.

PubMed

Goto, Yasushi; Sekine, Ikuo; Sekiguchi, Hiroshi; Yamada, Kazuhiko; Nokihara, Hiroshi; Yamamoto, Noboru; Kunitoh, Hideo; Ohe, Yuichiro; Tamura, Tomohide

2009-07-01

Quality of information available over the Internet has been a cause for concern. Our goal was to evaluate the quality of information available on lung cancer in the United States and Japan and assess the differences between the two. We conducted a prospective, observational Web review by searching the word "lung cancer" in Japanese and English, using Google Japan (Google-J), Google United States (Google-U), and Yahoo Japan (Yahoo-J). The first 50 Web sites displayed were evaluated from the ethical perspective and for the validity of the information. The administrator of each Web site was also investigated. Ethical policies were generally well described in the Web sites displayed by Google-U but less well so in the sites displayed by Google-J and Yahoo-J. The differences in the validity of the information available was more striking, in that 80% of the Web sites generated by Google-U described the most appropriate treatment methods, whereas less than 50% of the Web sites displayed by Google-J and Yahoo-J recommended the standard therapy, and more than 10% advertised alternative therapy. Nonprofit organizations and public institutions were the primary Web site administrators in the United States, whereas commercial or personal Web sites were more frequent in Japan. Differences in the quality of information on lung cancer available over the Internet were apparent between Japan and the United States. The reasons for such differences might be tracked to the administrators of the Web sites. Nonprofit organizations and public institutions are the up-and-coming Web site administrators for relaying reliable medical information.

Streamflow monitoring and statistics for development of water rights claims for Wild and Scenic Rivers, Owyhee Canyonlands Wilderness, Idaho, 2012

USGS Publications Warehouse

Wood, Molly S.; Fosness, Ryan L.

2013-01-01

The U.S. Geological Survey, in cooperation with the Bureau of Land Management (BLM), collected streamflow data in 2012 and estimated streamflow statistics for stream segments designated "Wild," "Scenic," or "Recreational" under the National Wild and Scenic Rivers System in the Owyhee Canyonlands Wilderness in southwestern Idaho. The streamflow statistics were used by BLM to develop and file a draft, federal reserved water right claim in autumn 2012 to protect federally designated "outstanding remarkable values" in the stream segments. BLM determined that the daily mean streamflow equaled or exceeded 20 and 80 percent of the time during bimonthly periods (two periods per month) and the bankfull streamflow are important streamflow thresholds for maintaining outstanding remarkable values. Prior to this study, streamflow statistics estimated using available datasets and tools for the Owyhee Canyonlands Wilderness were inaccurate for use in the water rights claim. Streamflow measurements were made at varying intervals during February–September 2012 at 14 monitoring sites; 2 of the monitoring sites were equipped with telemetered streamgaging equipment. Synthetic streamflow records were created for 11 of the 14 monitoring sites using a partial‑record method or a drainage-area-ratio method. Streamflow records were obtained directly from an operating, long-term streamgage at one monitoring site, and from discontinued streamgages at two monitoring sites. For 10 sites analyzed using the partial-record method, discrete measurements were related to daily mean streamflow at a nearby, telemetered “index” streamgage. Resulting regression equations were used to estimate daily mean and annual peak streamflow at the monitoring sites during the full period of record for the index sites. A synthetic streamflow record for Sheep Creek was developed using a drainage-area-ratio method, because measured streamflows did not relate well to any index site to allow use of the partial-record method. The synthetic and actual daily mean streamflow records were used to estimate daily mean streamflow that was exceeded 80, 50, and 20 percent of the time (80-, 50-, and 20-percent exceedances) for bimonthly and annual periods. Bankfull streamflow statistics were calculated by fitting the synthetic and actual annual peak streamflow records to a log Pearson Type III distribution using Bulletin 17B guidelines in the U.S. Geological Survey PeakFQ program. The coefficients of determination (R2) for the regressions between the monitoring and index sites ranged from 0.74 for Wickahoney Creek to 0.98 for the West Fork Bruneau River and Deep Creek. Confidence in computed streamflow statistics is highest among other sites for the East Fork Owyhee River and the West Fork Bruneau River on the basis of regression statistics, visual fit of the related data, and the range and number of streamflow measurements. Streamflow statistics for sites with the greatest uncertainty included Big Jacks, Little Jacks, Cottonwood, Wickahoney, and Sheep Creeks. The uncertainty in computed streamflow statistics was due to a number of factors which included the distance of index sites relative to monitoring sites, relatively low streamflow conditions that occurred during the study, and the limited number and range of streamflow measurements. However, the computed streamflow statistics are considered the best possible estimates given available datasets in the remote study area. Streamflow measurements over a wider range of hydrologic and climatic conditions would improve the relations between streamflow characteristics at monitoring and index sites. Additionally, field surveys are needed to verify if the streamflows selected for the water rights claims are sufficient for maintaining outstanding remarkable values in the Wild and Scenic rivers included in the study.

75 FR 3914 - Notice of Proposed Information Collection for 1004-0019

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-25

... handling and caring for domestic livestock authorized by the BLM to graze on public lands. This information... for the management and care of permitted livestock on grazing allotments. The BLM implements this...

43 CFR 3262.13 - May BLM require me to follow a well spacing program?

Code of Federal Regulations, 2011 CFR

2011-10-01

... RESOURCE LEASING Conducting Drilling Operations § 3262.13 May BLM require me to follow a well spacing... spacing: (a) Hydrologic, geologic, and reservoir characteristics of the field, minimizing well...

Systematic Review of Quality of Patient Information on Liposuction in the Internet

PubMed Central

Zuk, Grzegorz; Eylert, Gertraud; Raptis, Dimitri Aristotle; Guggenheim, Merlin; Shafighi, Maziar

2016-01-01

Background: A large number of patients who are interested in esthetic surgery actively search the Internet, which represents nowadays the first source of information. However, the quality of information available in the Internet on liposuction is currently unknown. The aim of this study was to assess the quality of patient information on liposuction available in the Internet. Methods: The quantitative and qualitative assessment of Web sites was based on a modified Ensuring Quality Information for Patients tool (36 items). Five hundred Web sites were identified by the most popular web search engines. Results: Two hundred forty-five Web sites were assessed after duplicates and irrelevant sources were excluded. Only 72 (29%) Web sites addressed >16 items, and scores tended to be higher for professional societies, portals, patient groups, health departments, and academic centers than for Web sites developed by physicians, respectively. The Ensuring Quality Information for Patients score achieved by Web sites ranged between 8 and 29 of total 36 points, with a median value of 16 points (interquartile range, 14–18). The top 10 Web sites with the highest scores were identified. Conclusions: The quality of patient information on liposuction available in the Internet is poor, and existing Web sites show substantial shortcomings. There is an urgent need for improvement in offering superior quality information on liposuction for patients intending to undergo this procedure. PMID:27482498

Systematic Review of Quality of Patient Information on Liposuction in the Internet.

PubMed

Zuk, Grzegorz; Palma, Adrian Fernando; Eylert, Gertraud; Raptis, Dimitri Aristotle; Guggenheim, Merlin; Shafighi, Maziar

2016-06-01

A large number of patients who are interested in esthetic surgery actively search the Internet, which represents nowadays the first source of information. However, the quality of information available in the Internet on liposuction is currently unknown. The aim of this study was to assess the quality of patient information on liposuction available in the Internet. The quantitative and qualitative assessment of Web sites was based on a modified Ensuring Quality Information for Patients tool (36 items). Five hundred Web sites were identified by the most popular web search engines. Two hundred forty-five Web sites were assessed after duplicates and irrelevant sources were excluded. Only 72 (29%) Web sites addressed >16 items, and scores tended to be higher for professional societies, portals, patient groups, health departments, and academic centers than for Web sites developed by physicians, respectively. The Ensuring Quality Information for Patients score achieved by Web sites ranged between 8 and 29 of total 36 points, with a median value of 16 points (interquartile range, 14-18). The top 10 Web sites with the highest scores were identified. The quality of patient information on liposuction available in the Internet is poor, and existing Web sites show substantial shortcomings. There is an urgent need for improvement in offering superior quality information on liposuction for patients intending to undergo this procedure.

Using Web sites on quality health care for teaching consumers in public libraries.

PubMed

Oermann, Marilyn H; Lesley, Marsha L; VanderWal, Jillon S

2005-01-01

More and more consumers are searching the Internet for health information. Health Web sites vary in quality, though, and not all consumers are aware of the need to evaluate the information they find on the Web. Nurses and other health providers involved in patient education can evaluate Web sites and suggest quality sites for patients to use. This article describes a project we implemented in 2 public libraries to educate consumers about quality health care and patient safety using Web sites that we had evaluated earlier. Participants (n = 103) completed resources on health care quality, questions patients should ask about their diagnoses and treatment options, changes in Medicare and Medicare options or ways to make their health benefits work for them, and tips to help prevent medical errors. Most consumers were highly satisfied with the Web sites and the information they learned on quality care from these resources. Many participants did not have Internet access at home or work and instead used the library to search the Web. Information about the Web sites used in this project and other sites on quality care can be made available in libraries and community settings and as part of patient education resources in hospitals. The Web provides easy access for consumers to information about patient safety initiatives and health care quality in general.

Building a Playground: General Guidelines for Creating Educational Web Sites for Children

PubMed Central

Meloncon, Lisa; Haynes, Erin; Varelmann, Megan; Groh, Lisa

2015-01-01

Purpose Since 2004, the number of children online has increased 18%, compared with a 10% increase in total users. Not only do children represent a growing segment of Internet users, much of what they do online has a specific purpose: education. To help technical communicators create educational Web sites for children, we offer a set of guidelines to direct the design process. Method Nine children participated in a usability test of the CARES Playground, an educational Web site geared toward 7- to 9-year-olds. The site was designed by a group of graduate students in professional writing based on a review of the (admittedly limited) literature dealing with designing Web sites for children. This paper matches common themes from existing literature to the results of the usability tests. Results Since all the information on designing Web sites for children emerged from the literature of designing Web sites for adults, the themes of navigation, appearance, and content are not unfamiliar. However, the interpretation of those common issues for children—as well as the children’s reaction to them—may be surprising. Conclusion Technical communicators need to be conscious and deliberate when designing Web sites for children. To ensure that educational Web sites are able to meet their learning goals, careful consideration of children’s developmental abilities and Web preferences must be considered. We present several guidelines as a starting point, though further research is needed to confirm and expand upon them. PMID:26633909