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Sample records for blue image planes

  1. Fourier plane imaging microscopy

    SciTech Connect

    Dominguez, Daniel Peralta, Luis Grave de; Alharbi, Nouf; Alhusain, Mdhaoui; Bernussi, Ayrton A.

    2014-09-14

    We show how the image of an unresolved photonic crystal can be reconstructed using a single Fourier plane (FP) image obtained with a second camera that was added to a traditional compound microscope. We discuss how Fourier plane imaging microscopy is an application of a remarkable property of the obtained FP images: they contain more information about the photonic crystals than the images recorded by the camera commonly placed at the real plane of the microscope. We argue that the experimental results support the hypothesis that surface waves, contributing to enhanced resolution abilities, were optically excited in the studied photonic crystals.

  2. Fourier plane image amplifier

    DOEpatents

    Hackel, L.A.; Hermann, M.R.; Dane, C.B.; Tiszauer, D.H.

    1995-12-12

    A solid state laser is frequency tripled to 0.3 {micro}m. A small portion of the laser is split off and generates a Stokes seed in a low power oscillator. The low power output passes through a mask with the appropriate hole pattern. Meanwhile, the bulk of the laser output is focused into a larger stimulated Brillouin scattering (SBS) amplifier. The low power beam is directed through the same cell in the opposite direction. The majority of the amplification takes place at the focus which is the fourier transform plane of the mask image. The small holes occupy large area at the focus and thus are preferentially amplified. The amplified output is now imaged onto the multichip module where the holes are drilled. Because of the fourier plane amplifier, only about 1/10th the power of a competitive system is needed. This concept allows less expensive masks to be used in the process and requires much less laser power. 1 fig.

  3. Fourier plane image amplifier

    DOEpatents

    Hackel, Lloyd A.; Hermann, Mark R.; Dane, C. Brent; Tiszauer, Detlev H.

    1995-01-01

    A solid state laser is frequency tripled to 0.3 .mu.m. A small portion of the laser is split off and generates a Stokes seed in a low power oscillator. The low power output passes through a mask with the appropriate hole pattern. Meanwhile, the bulk of the laser output is focused into a larger stimulated Brillouin scattering (SBS) amplifier. The low power beam is directed through the same cell in the opposite direction. The majority of the amplification takes place at the focus which is the fourier transform plane of the mask image. The small holes occupy large area at the focus and thus are preferentially amplified. The amplified output is now imaged onto the multichip module where the holes are drilled. Because of the fourier plane amplifier, only .about.1/10th the power of a competitive system is needed. This concept allows less expensive masks to be used in the process and requires much less laser power.

  4. Mongolian blue spots (image)

    MedlinePlus

    Mongolian blue spots are flat bluish- to bluish-gray skin markings commonly appearing at birth or shortly ... back and also can appear on the shoulders. Mongolian spots are benign and are not associated with ...

  5. Image plane sweep volume illumination.

    PubMed

    Sundén, Erik; Ynnerman, Anders; Ropinski, Timo

    2011-12-01

    In recent years, many volumetric illumination models have been proposed, which have the potential to simulate advanced lighting effects and thus support improved image comprehension. Although volume ray-casting is widely accepted as the volume rendering technique which achieves the highest image quality, so far no volumetric illumination algorithm has been designed to be directly incorporated into the ray-casting process. In this paper we propose image plane sweep volume illumination (IPSVI), which allows the integration of advanced illumination effects into a GPU-based volume ray-caster by exploiting the plane sweep paradigm. Thus, we are able to reduce the problem complexity and achieve interactive frame rates, while supporting scattering as well as shadowing. Since all illumination computations are performed directly within a single rendering pass, IPSVI does not require any preprocessing nor does it need to store intermediate results within an illumination volume. It therefore has a significantly lower memory footprint than other techniques. This makes IPSVI directly applicable to large data sets. Furthermore, the integration into a GPU-based ray-caster allows for high image quality as well as improved rendering performance by exploiting early ray termination. This paper discusses the theory behind IPSVI, describes its implementation, demonstrates its visual results and provides performance measurements.

  6. Tilted planes in 3D image analysis

    NASA Astrophysics Data System (ADS)

    Pargas, Roy P.; Staples, Nancy J.; Malloy, Brian F.; Cantrell, Ken; Chhatriwala, Murtuza

    1998-03-01

    Reliable 3D wholebody scanners which output digitized 3D images of a complete human body are now commercially available. This paper describes a software package, called 3DM, being developed by researchers at Clemson University and which manipulates and extracts measurements from such images. The focus of this paper is on tilted planes, a 3DM tool which allows a user to define a plane through a scanned image, tilt it in any direction, and effectively define three disjoint regions on the image: the points on the plane and the points on either side of the plane. With tilted planes, the user can accurately take measurements required in applications such as apparel manufacturing. The user can manually segment the body rather precisely. Tilted planes assist the user in analyzing the form of the body and classifying the body in terms of body shape. Finally, titled planes allow the user to eliminate extraneous and unwanted points often generated by a 3D scanner. This paper describes the user interface for tilted planes, the equations defining the plane as the user moves it through the scanned image, an overview of the algorithms, and the interaction of the tilted plane feature with other tools in 3DM.

  7. Calculation of confocal microscope images of cholesteric blue phases

    NASA Astrophysics Data System (ADS)

    Fukuda, Jun-ichi; Okumura, Yasushi; Kikuchi, Hirotsugu

    2016-03-01

    Real-space images of bulk cholesteric blue phases (BPs) have been successfully obtained by confocal microscopy observations using structural color without doping fluorescent dye. However, theoretical interpretation of these images (for example, the understanding of the relation between intensity distribution and the ordering of BPs) remains challenging because typical lattice spacing of BPs is of the order of the wavelength of visible light, and therefore geometrical optics is entirely useless. In this work, we present a numerical approach to calculate the confocal images of BPs by solving the Maxwell equations. Calculated confocal images are consistent with experimental observations in terms of in-plane symmetry.

  8. Image-plane processing of visual information

    NASA Technical Reports Server (NTRS)

    Huck, F. O.; Fales, C. L.; Park, S. K.; Samms, R. W.

    1984-01-01

    Shannon's theory of information is used to optimize the optical design of sensor-array imaging systems which use neighborhood image-plane signal processing for enhancing edges and compressing dynamic range during image formation. The resultant edge-enhancement, or band-pass-filter, response is found to be very similar to that of human vision. Comparisons of traits in human vision with results from information theory suggest that: (1) Image-plane processing, like preprocessing in human vision, can improve visual information acquisition for pattern recognition when resolving power, sensitivity, and dynamic range are constrained. Improvements include reduced sensitivity to changes in lighter levels, reduced signal dynamic range, reduced data transmission and processing, and reduced aliasing and photosensor noise degradation. (2) Information content can be an appropriate figure of merit for optimizing the optical design of imaging systems when visual information is acquired for pattern recognition. The design trade-offs involve spatial response, sensitivity, and sampling interval.

  9. Hybrid Image-Plane/Stereo Manipulation

    NASA Technical Reports Server (NTRS)

    Baumgartner, Eric; Robinson, Matthew

    2004-01-01

    Hybrid Image-Plane/Stereo (HIPS) manipulation is a method of processing image data, and of controlling a robotic manipulator arm in response to the data, that enables the manipulator arm to place an end-effector (an instrument or tool) precisely with respect to a target (see figure). Unlike other stereoscopic machine-vision-based methods of controlling robots, this method is robust in the face of calibration errors and changes in calibration during operation. In this method, a stereoscopic pair of cameras on the robot first acquires images of the manipulator at a set of predefined poses. The image data are processed to obtain image-plane coordinates of known visible features of the end-effector. Next, there is computed an initial calibration in the form of a mapping between (1) the image-plane coordinates and (2) the nominal three-dimensional coordinates of the noted end-effector features in a reference frame fixed to the main robot body at the base of the manipulator. The nominal three-dimensional coordinates are obtained by use of the nominal forward kinematics of the manipulator arm that is, calculated by use of the currently measured manipulator joint angles and previously measured lengths of manipulator arm segments under the assumption that the arm segments are rigid, that the arm lengths are constant, and that there is no backlash. It is understood from the outset that these nominal three-dimensional coordinates are likely to contain possibly significant calibration errors, but the effects of the errors are progressively reduced, as described next. As the end-effector is moved toward the target, the calibration is updated repeatedly by use of data from newly acquired images of the end-effector and of the corresponding nominal coordinates in the manipulator reference frame. By use of the updated calibration, the coordinates of the target are computed in manipulator-reference-frame coordinates and then used to the necessary manipulator joint angles to position

  10. Scanned Image Projection System Employing Intermediate Image Plane

    NASA Technical Reports Server (NTRS)

    DeJong, Christian Dean (Inventor); Hudman, Joshua M. (Inventor)

    2014-01-01

    In imaging system, a spatial light modulator is configured to produce images by scanning a plurality light beams. A first optical element is configured to cause the plurality of light beams to converge along an optical path defined between the first optical element and the spatial light modulator. A second optical element is disposed between the spatial light modulator and a waveguide. The first optical element and the spatial light modulator are arranged such that an image plane is created between the spatial light modulator and the second optical element. The second optical element is configured to collect the diverging light from the image plane and collimate it. The second optical element then delivers the collimated light to a pupil at an input of the waveguide.

  11. Cell imaging techniques based on digital image plane holography

    NASA Astrophysics Data System (ADS)

    Chen, Zhaoji; Gong, Wendi; Liu, Feifei; Wang, Huaying

    2010-11-01

    This paper has further studied the implementation methods and recording conditions of digital microscopic image plane holography (DMIPH). Two optical systems of DMIPH were built: one is recording hologram by using plane waves as reference light, the other is recording hologram by spherical reference light. Breast cancer cells and USAF resolution test target is used as tested samples in the experiment. Then the intensity distribution and three-dimensional shape information of the cells are got accurately. The experiment results show that DMIPH avoids the process of finding recording distance by using auto-focusing approach. The recording and reconstruction process of DMIPH is simple. Therefore DMIPH can be applied to the microscopic imaging of cells more effectively.

  12. Fuzzy logic color detection: Blue areas in melanoma dermoscopy images.

    PubMed

    Lingala, Mounika; Stanley, R Joe; Rader, Ryan K; Hagerty, Jason; Rabinovitz, Harold S; Oliviero, Margaret; Choudhry, Iqra; Stoecker, William V

    2014-07-01

    Fuzzy logic image analysis techniques were used to analyze three shades of blue (lavender blue, light blue, and dark blue) in dermoscopic images for melanoma detection. A logistic regression model provided up to 82.7% accuracy for melanoma discrimination for 866 images. With a support vector machines (SVM) classifier, lower accuracy was obtained for individual shades (79.9-80.1%) compared with up to 81.4% accuracy with multiple shades. All fuzzy blue logic alpha cuts scored higher than the crisp case. Fuzzy logic techniques applied to multiple shades of blue can assist in melanoma detection. These vector-based fuzzy logic techniques can be extended to other image analysis problems involving multiple colors or color shades.

  13. General image method in a plane-layered elastostatic medium

    NASA Technical Reports Server (NTRS)

    Fares, N.; Li, V. C.

    1988-01-01

    The general-image method presently used to obtain the elastostatic fields in plane-layered media relies on the use of potentials in order to represent elastic fields. For the case of a single interface, this method yields the displacement field in closed form, and is applicable to antiplane, plane, and three-dimensional problems. In the case of multiplane interfaces, the image method generates the displacement fields in terms of infinite series whose convergences can be accelerated to improve method efficiency.

  14. Resolution of oblique-plane images in sectioning microscopy.

    PubMed

    Smith, C W; Botcherby, E J; Wilson, T

    2011-01-31

    Live biological specimens exhibit time-varying behavior on the microscale in all three dimensions. Although scanning confocal and two-photon microscopes are able to record three-dimensional image stacks through these specimens, they do so at relatively low speeds which limits the time resolution of the biological processes that can be observed. One way to improve the data acquisition rate is to image only the regions of a specimen that are of interest and so researchers have recently begun to acquire two-dimensional images of inclined planes or surfaces extending significantly into the z-direction. As the resolution is not uniform in x, y and z, the images possess non-isotropic resolution. We explore this theoretically and show that images of an oblique plane may contain spectral content that could not have been generated by specimen features lying wholly within the plane but must instead arise from a spatial variation in another direction. In some cases we find that the image contains frequencies three times higher than the resolution limit for in-plane features. We confirm this finding through numerical simulations and experiments on a novel, oblique-plane imaging system and suggest that care be taken in the interpretation of such images.

  15. Coded excitation plane wave imaging for shear wave motion detection.

    PubMed

    Song, Pengfei; Urban, Matthew W; Manduca, Armando; Greenleaf, James F; Chen, Shigao

    2015-07-01

    Plane wave imaging has greatly advanced the field of shear wave elastography thanks to its ultrafast imaging frame rate and the large field-of-view (FOV). However, plane wave imaging also has decreased penetration due to lack of transmit focusing, which makes it challenging to use plane waves for shear wave detection in deep tissues and in obese patients. This study investigated the feasibility of implementing coded excitation in plane wave imaging for shear wave detection, with the hypothesis that coded ultrasound signals can provide superior detection penetration and shear wave SNR compared with conventional ultrasound signals. Both phase encoding (Barker code) and frequency encoding (chirp code) methods were studied. A first phantom experiment showed an approximate penetration gain of 2 to 4 cm for the coded pulses. Two subsequent phantom studies showed that all coded pulses outperformed the conventional short imaging pulse by providing superior sensitivity to small motion and robustness to weak ultrasound signals. Finally, an in vivo liver case study on an obese subject (body mass index = 40) demonstrated the feasibility of using the proposed method for in vivo applications, and showed that all coded pulses could provide higher SNR shear wave signals than the conventional short pulse. These findings indicate that by using coded excitation shear wave detection, one can benefit from the ultrafast imaging frame rate and large FOV provided by plane wave imaging while preserving good penetration and shear wave signal quality, which is essential for obtaining robust shear elasticity measurements of tissue.

  16. 3D fluorescence anisotropy imaging using selective plane illumination microscopy

    PubMed Central

    Hedde, Per Niklas; Ranjit, Suman; Gratton, Enrico

    2015-01-01

    Fluorescence anisotropy imaging is a popular method to visualize changes in organization and conformation of biomolecules within cells and tissues. In such an experiment, depolarization effects resulting from differences in orientation, proximity and rotational mobility of fluorescently labeled molecules are probed with high spatial resolution. Fluorescence anisotropy is typically imaged using laser scanning and epifluorescence-based approaches. Unfortunately, those techniques are limited in either axial resolution, image acquisition speed, or by photobleaching. In the last decade, however, selective plane illumination microscopy has emerged as the preferred choice for three-dimensional time lapse imaging combining axial sectioning capability with fast, camera-based image acquisition, and minimal light exposure. We demonstrate how selective plane illumination microscopy can be utilized for three-dimensional fluorescence anisotropy imaging of live cells. We further examined the formation of focal adhesions by three-dimensional time lapse anisotropy imaging of CHO-K1 cells expressing an EGFP-paxillin fusion protein. PMID:26368202

  17. Determining Plane Mirror Image Distance from Eye Charts.

    ERIC Educational Resources Information Center

    Lapp, David R.

    1993-01-01

    Presents a method to convince students that the image produced by a plane mirror is actually behind the mirror. Uses observations that the letters of an eye chart posted on a mirror are twice the size of the images of letters of an eye chart they are holding. Provides two reproducible eye charts. (MDH)

  18. Imaging scatterer planes by photoelectron diffraction

    NASA Astrophysics Data System (ADS)

    Seelmann-Eggebert, M.

    1997-04-01

    A novel direct crystallographic method CHRISDA (combined holographic real-space imaging by superimposed dimer function algorithm) is proposed which permits an assessment of the near-surface structure of a solid sample by analysis of a single core-level photoemission or Auger emission diffraction pattern (XPD or AED) recorded over the hemisphere of electron escape angles. Combining the elements of holography and real-space triangulation, the approach achieves a high spatial resolution (≈0.1 Å) and requires a knowledge of only a few non-structural parameters. To demonstrate the experimental efficacy of CHRISDA, a Sn film deposited on a CdTe(111) substrate is analyzed and yields the diamond structure characteristic of α-Sn.

  19. Analysis of nulling phase functions suitable to image plane coronagraphy

    NASA Astrophysics Data System (ADS)

    Hénault, François; Carlotti, Alexis; Vérinaud, Christophe

    2016-07-01

    Coronagraphy is a very efficient technique for identifying and characterizing extra-solar planets orbiting in the habitable zone of their parent star, especially in a space environment. An important family of coronagraphs is actually based on phase plates located at an intermediate image plane of the optical system, and spreading the starlight outside the "Lyot" exit pupil plane of the instrument. In this commutation we present a set of candidate phase functions generating a central null at the Lyot plane, and study how it propagates to the image plane of the coronagraph. These functions include linear azimuthal phase ramps (the well-known optical vortex), azimuthally cosine-modulated phase profiles, and circular phase gratings. Nnumerical simulations of the expected null depth, inner working angle, sensitivity to pointing errors, effect of central obscuration located at the pupil or image planes, and effective throughput including image mask and Lyot stop transmissions are presented and discussed. The preliminary conclusion is that azimuthal cosine functions appear as an interesting alternative to the classical optical vortex of integer topological charge.

  20. MR imaging of blue rubber bleb nevus syndrome.

    PubMed

    Jorizzo, J R; Amparo, E G

    1986-01-01

    This report describes the use of magnetic resonance imaging in the evaluation of a patient with blue rubber bleb nevus syndrome, a rare entity consisting of multiple cutaneous and visceral hemangiomas. The bright signal obtained on T2-weighted images is probably the result of slow flow or thrombosis, typically present in these lesions, and allows for easy recognition.

  1. Coded Excitation Plane Wave Imaging for Shear Wave Motion Detection

    PubMed Central

    Song, Pengfei; Urban, Matthew W.; Manduca, Armando; Greenleaf, James F.; Chen, Shigao

    2015-01-01

    Plane wave imaging has greatly advanced the field of shear wave elastography thanks to its ultrafast imaging frame rate and the large field-of-view (FOV). However, plane wave imaging also has decreased penetration due to lack of transmit focusing, which makes it challenging to use plane waves for shear wave detection in deep tissues and in obese patients. This study investigated the feasibility of implementing coded excitation in plane wave imaging for shear wave detection, with the hypothesis that coded ultrasound signals can provide superior detection penetration and shear wave signal-to-noise-ratio (SNR) compared to conventional ultrasound signals. Both phase encoding (Barker code) and frequency encoding (chirp code) methods were studied. A first phantom experiment showed an approximate penetration gain of 2-4 cm for the coded pulses. Two subsequent phantom studies showed that all coded pulses outperformed the conventional short imaging pulse by providing superior sensitivity to small motion and robustness to weak ultrasound signals. Finally, an in vivo liver case study on an obese subject (Body Mass Index = 40) demonstrated the feasibility of using the proposed method for in vivo applications, and showed that all coded pulses could provide higher SNR shear wave signals than the conventional short pulse. These findings indicate that by using coded excitation shear wave detection, one can benefit from the ultrafast imaging frame rate and large FOV provided by plane wave imaging while preserving good penetration and shear wave signal quality, which is essential for obtaining robust shear elasticity measurements of tissue. PMID:26168181

  2. Navigating solid medical images by pencils of sectioning planes

    NASA Astrophysics Data System (ADS)

    Bookstein, Fred L.; Athey, Brian D.; Green, William D. K.; Wetzel, Arthur W.

    2000-10-01

    Beyond their involvement in ordinary surface rendering, the boundaries of organs in medical images have differential properties that make them quite useful for quantitative understanding. In particular, their geometry affords a framework for navigating the original solid, representing its R3 contents quite flexibility as multiple pseudovolumes R2 x T, where T is ar eal-valued parameter standing for screen time. A navigation is a smoothly parameterized series of image sections characterized by normal direction, centerpoint, scale and orientation. Such filmstrips represent a radical generalization of conventional medical image dynamics. The lances encountered in these navigations can be represented by constructs from classic differential geometry. Sequences of plane sections can be formalized as continuous pencils of planes, sets of cardinality (infinity) 1 that are sometimes explicitly characterized by a real-value parameter and sometimes defined implicitly as the intersection (curve of common elements) of a pair of bundles of (infinity) 2 planes. An example of the first type of navigation is the pencil of planes through the tangent line at one point of a curve; of the second type, the cone of planes through a point tangent to a surface. The further enhancements of centering, orienting, and rescaling in the medical context are intended to leave landmark points or boundary intersections invariant on the screen. Edgewarp, a publicly available software package, allows free play with pencils of planes like these as they section one single enormous medical data resource, the Visible Human data sets from the National Library of Medicine. This paper argues the relative merits of such visualizations over conventional surface-rendered flybys for understanding and communication of associated anatomical knowledge.

  3. Characteristics of stereo images from detectors in focal plane array.

    PubMed

    Son, Jung-Young; Yeom, Seokwon; Chun, Joo-Hwan; Guschin, Vladmir P; Lee, Dong-Su

    2011-07-01

    The equivalent ray geometry of two horizontally aligned detectors at the focal plane of the main antenna in a millimeter wave imaging system is analyzed to reveal the reason why the images from the detectors are fused as an image with a depth sense. Scanning the main antenna in both horizontal and vertical directions makes each detector perform as a camera, and the two detectors can work like a stereo camera in the millimeter wave range. However, the stereo camera geometry is different from that of the stereo camera used in the visual spectral range because the detectors' viewing directions are diverging to each other and they are a certain distance apart. The depth sense is mainly induced by the distance between detectors. The images obtained from the detectors in the millimeter imaging system are perceived with a good depth sense. The disparities responsible for the depth sense are identified in the images.

  4. Fresnel diffraction in the case of an inclined image plane.

    PubMed

    Modregger, Peter; Lübbert, Daniel; Schäfer, Peter; Köhler, Rolf; Weitkamp, Timm; Hanke, Michael; Baumbach, Tilo

    2008-03-31

    An extension of the theoretical formalism of Fresnel diffraction to the case of an inclined image plane is proposed. The resulting numerical algorithm speeds up computation times by typically three orders of magnitude, thus opening the possibility of utilizing previously inapplicable image analysis algorithms for this special type of a non shift-invariant imaging system. This is exemplified by adapting an iterative phase retrieval algorithm developed for electron microscopy to the case of hard x-ray imaging with asymmetric Bragg reflection (the so-called "Bragg Magnifier"). Numerical simulations demonstrate the convergence and feasibility of the iterative phase retrieval algorithm for the case of x-ray imaging with the Bragg Magnifier.

  5. Stolt's f-k migration for plane wave ultrasound imaging.

    PubMed

    Garcia, Damien; Le Tarnec, Louis; Muth, Stéphan; Montagnon, Emmanuel; Porée, Jonathan; Cloutier, Guy

    2013-09-01

    Ultrafast ultrasound is an emerging modality that offers new perspectives and opportunities in medical imaging. Plane wave imaging (PWI) allows one to attain very high frame rates by transmission of planar ultrasound wave-fronts. As a plane wave reaches a given scatterer, the latter becomes a secondary source emitting upward spherical waves and creating a diffraction hyperbola in the received RF signals. To produce an image of the scatterers, all the hyperbolas must be migrated back to their apexes. To perform beamforming of plane wave echo RFs and return high-quality images at high frame rates, we propose a new migration method carried out in the frequency-wavenumber (f-k) domain. The f-k migration for PWI has been adapted from the Stolt migration for seismic imaging. This migration technique is based on the exploding reflector model (ERM), which consists in assuming that all the scatterers explode in concert and become acoustic sources. The classical ERM model, however, is not appropriate for PWI. We showed that the ERM can be made suitable for PWI by a spatial transformation of the hyperbolic traces present in the RF data. In vitro experiments were performed to outline the advantages of PWI with Stolt's f-k migration over the conventional delay-and-sum (DAS) approach. The Stolt's f-k migration was also compared with the Fourier-based method developed by J.-Y. Lu. Our findings show that multi-angle compounded f-k migrated images are of quality similar to those obtained with a stateof- the-art dynamic focusing mode. This remained true even with a very small number of steering angles, thus ensuring a highly competitive frame rate. In addition, the new FFT-based f-k migration provides comparable or better contrast-to-noise ratio and lateral resolution than the Lu's and DAS migration schemes. Matlab codes for the Stolt's f-k migration for PWI are provided.

  6. Image-plane processing for improved computer vision

    NASA Technical Reports Server (NTRS)

    Huck, F. O.; Fales, C. L.; Park, S. K.; Samms, R. W.

    1984-01-01

    The proper combination of optical design with image plane processing, as in the mechanism of human vision, which allows to improve the performance of sensor array imaging systems for edge detection and location was examined. Two dimensional bandpass filtering during image formation, optimizes edge enhancement and minimizes data transmission. It permits control of the spatial imaging system response to tradeoff edge enhancement for sensitivity at low light levels. It is shown that most of the information, up to about 94%, is contained in the signal intensity transitions from which the location of edges is determined for raw primal sketches. Shading the lens transmittance to increase depth of field and using a hexagonal instead of square sensor array lattice to decrease sensitivity to edge orientation improves edge information about 10%.

  7. Geometric Modeling With Image Plane Integral (IPI) Holography

    NASA Astrophysics Data System (ADS)

    Lacey, D. C. L.

    1984-10-01

    Holography has long been considered to be an ideal method of visually presenting three-dimensional data which has been either experimentally acquired or mathematically calculated with a preconceived form in mind. Unfortunately, holograms have not had the image quality and accuracy to gain scientific acceptability outside the field of interferometric analysis. This paper will describe the use of an advanced composite holographic technique to give a clear, autostereoscopic image of a computer designed automotive part and a computer graphics concept of a molecule. The holograms were manufactured using Image Plane Integral (IPI) holography on an Argon laser printer designed and built by the author. The final holograms exhibited bright, achromatic, real images with high resolution and a minimum amount of controllable distortion. Additionally, because the IPI method uses a 35mm microfilmstrip for input, it could be universally applied as a hard copy format for presentation of almost any type of three-dimensional data base.

  8. Lower-Dark-Current, Higher-Blue-Response CMOS Imagers

    NASA Technical Reports Server (NTRS)

    Pain, Bedabrata; Cunningham, Thomas; Hancock, Bruce

    2008-01-01

    Several improved designs for complementary metal oxide/semiconductor (CMOS) integrated-circuit image detectors have been developed, primarily to reduce dark currents (leakage currents) and secondarily to increase responses to blue light and increase signal-handling capacities, relative to those of prior CMOS imagers. The main conclusion that can be drawn from a study of the causes of dark currents in prior CMOS imagers is that dark currents could be reduced by relocating p/n junctions away from Si/SiO2 interfaces. In addition to reflecting this conclusion, the improved designs include several other features to counteract dark-current mechanisms and enhance performance.

  9. Achromatic Focal Plane Mask for Exoplanet Imaging Coronagraphy

    NASA Technical Reports Server (NTRS)

    Newman, Kevin Edward; Belikov, Ruslan; Guyon, Olivier; Balasubramanian, Kunjithapatham; Wilson, Dan

    2013-01-01

    Recent advances in coronagraph technologies for exoplanet imaging have achieved contrasts close to 1e10 at 4 lambda/D and 1e-9 at 2 lambda/D in monochromatic light. A remaining technological challenge is to achieve high contrast in broadband light; a challenge that is largely limited by chromaticity of the focal plane mask. The size of a star image scales linearly with wavelength. Focal plane masks are typically the same size at all wavelengths, and must be sized for the longest wavelength in the observational band to avoid starlight leakage. However, this oversized mask blocks useful discovery space from the shorter wavelengths. We present here the design, development, and testing of an achromatic focal plane mask based on the concept of optical filtering by a diffractive optical element (DOE). The mask consists of an array of DOE cells, the combination of which functions as a wavelength filter with any desired amplitude and phase transmission. The effective size of the mask scales nearly linearly with wavelength, and allows significant improvement in the inner working angle of the coronagraph at shorter wavelengths. The design is applicable to almost any coronagraph configuration, and enables operation in a wider band of wavelengths than would otherwise be possible. We include initial results from a laboratory demonstration of the mask with the Phase Induced Amplitude Apodization coronagraph.

  10. Edge detection - Image-plane versus digital processing

    NASA Technical Reports Server (NTRS)

    Huck, Friedrich O.; Fales, Carl L.; Park, Stephen K.; Triplett, Judith A.

    1987-01-01

    To optimize edge detection with the familiar Laplacian-of-Gaussian operator, it has become common to implement this operator with a large digital convolution mask followed by some interpolation of the processed data to determine the zero crossings that locate edges. It is generally recognized that this large mask causes substantial blurring of fine detail. It is shown that the spatial detail can be improved by a factor of about four with either the Wiener-Laplacian-of-Gaussian filter or an image-plane processor. The Wiener-Laplacian-of-Gaussian filter minimizes the image-gathering degradations if the scene statistics are at least approximately known and also serves as an interpolator to determine the desired zero crossings directly. The image-plane processor forms the Laplacian-of-Gaussian response by properly combining the optical design of the image-gathering system with a minimal three-by-three lateral-inhibitory processing mask. This approach, which is suggested by Marr's model of early processing in human vision, also reduces data processing by about two orders of magnitude and data transmission by up to an order of magnitude.

  11. Feature-aided multiple target tracking in the image plane

    NASA Astrophysics Data System (ADS)

    Brown, Andrew P.; Sullivan, Kevin J.; Miller, David J.

    2006-05-01

    Vast quantities of EO and IR data are collected on airborne platforms (manned and unmanned) and terrestrial platforms (including fixed installations, e.g., at street intersections), and can be exploited to aid in the global war on terrorism. However, intelligent preprocessing is required to enable operator efficiency and to provide commanders with actionable target information. To this end, we have developed an image plane tracker which automatically detects and tracks multiple targets in image sequences using both motion and feature information. The effects of platform and camera motion are compensated via image registration, and a novel change detection algorithm is applied for accurate moving target detection. The contiguous pixel blob on each moving target is segmented for use in target feature extraction and model learning. Feature-based target location measurements are used for tracking through move-stop-move maneuvers, close target spacing, and occlusion. Effective clutter suppression is achieved using joint probabilistic data association (JPDA), and confirmed target tracks are indicated for further processing or operator review. In this paper we describe the algorithms implemented in the image plane tracker and present performance results obtained with video clips from the DARPA VIVID program data collection and from a miniature unmanned aerial vehicle (UAV) flight.

  12. Uncooled infrared focal plane array imaging in China

    NASA Astrophysics Data System (ADS)

    Lei, Shuyu

    2015-06-01

    This article reviews the development of uncooled infrared focal plane array (UIFPA) imaging in China in the past decade. Sensors based on optical or electrical read-out mechanism were developed but the latter dominates the market. In resistive bolometers, VOx and amorphous silicon are still the two major thermal-sensing materials. The specifications of the IRFPA made by different manufactures were collected and compared. Currently more than five Chinese companies and institutions design and fabricate uncooled infrared focal plane array. Some devices have sensitivity as high as 30 mK; the largest array for commercial products is 640×512 and the smallest pixel size is 17 μm. Emphasis is given on the pixel MEMS design, ROIC design, fabrication, and packaging of the IRFPA manufactured by GWIC, especially on design for high sensitivities, low noise, better uniformity and linearity, better stabilization for whole working temperature range, full-digital design, etc.

  13. Simultaneous multi-plane imaging of neural circuits

    PubMed Central

    Yang, Weijian; Miller, Jae-eun Kang; Carrillo-Reid, Luis; Pnevmatikakis, Eftychios; Paninski, Liam; Yuste, Rafael; Peterka, Darcy S.

    2015-01-01

    Summary Recording the activity of large populations of neurons is an important step towards understanding the emergent function of neural circuits. Here we present a simple holographic method to simultaneously perform two-photon calcium imaging of neuronal populations across multiple areas and layers of mouse cortex in vivo. We use prior knowledge of neuronal locations, activity sparsity and a constrained nonnegative matrix factorization algorithm to extract signals from neurons imaged simultaneously and located in different focal planes or fields of view. Our laser multiplexing approach is simple and fast and could be used as a general method to image the activity of neural circuits in three dimensions across multiple areas in the brain. PMID:26774159

  14. Radiometric infrared focal plane array imaging system for thermographic applications

    NASA Technical Reports Server (NTRS)

    Esposito, B. J.; Mccafferty, N.; Brown, R.; Tower, J. R.; Kosonocky, W. F.

    1992-01-01

    This document describes research performed under the Radiometric Infrared Focal Plane Array Imaging System for Thermographic Applications contract. This research investigated the feasibility of using platinum silicide (PtSi) Schottky-barrier infrared focal plane arrays (IR FPAs) for NASA Langley's specific radiometric thermal imaging requirements. The initial goal of this design was to develop a high spatial resolution radiometer with an NETD of 1 percent of the temperature reading over the range of 0 to 250 C. The proposed camera design developed during this study and described in this report provides: (1) high spatial resolution (full-TV resolution); (2) high thermal dynamic range (0 to 250 C); (3) the ability to image rapid, large thermal transients utilizing electronic exposure control (commandable dynamic range of 2,500,000:1 with exposure control latency of 33 ms); (4) high uniformity (0.5 percent nonuniformity after correction); and (5) high thermal resolution (0.1 C at 25 C background and 0.5 C at 250 C background).

  15. Concurrent Monitoring of In-plane Strain and Out-of-plane Displacement of Tire Using Digital Image Correlation Method

    NASA Astrophysics Data System (ADS)

    Hiraoka, Naoki; Matsuzaki, Ryosuke; Todoroki, Akira

    In order to improve performance of anti lock brake system (ABS) and detect condition of road surface, intelligent tires that monitor strain of interior surface and rolling radius of tire are demanded. However, the high stiffness of an attached sensor like a strain gauge causes debonding of sensors from tire rubber. In the present study, noncontact concurrent monitoring method is proposed using digital image correlation method (DICM) and spotlight projection. In-plane strain and out-of-plane displacement (rolling radius) are calculated by using image processing with an image of interior surface of tire that is taken with a single CCD camera fixed on wheel rim. New monitoring system is applied to Al beam and commercially available radial tire. As a result, this monitoring system is proved to be able to measure in-plane strain and out-of-plane displacement with high accuracy, and confirmed to be effective for concurrent monitoring of tires.

  16. In vivo molecular contrast OCT imaging of methylene blue.

    PubMed

    Kim, Wihan; Applegate, Brian E

    2015-04-01

    An 830-nm spectral-domain optical coherence tomography (OCT) system with an integrated 663-nm diode pump laser has been developed to enable molecular contrast OCT imaging of methylene blue (MB), a common vital dye used clinically. The introduction of the 663-nm diode laser, which acts as the pump in this implementation of pump-probe OCT (PPOCT), represents a minor modification to an otherwise typical OCT system. A newly developed background subtraction technique completely removes all background from intensity noise at the pump modulation frequency, simplifying the interpretation of PPOCT images. These developments have enabled the first in vivo imaging of MB with PPOCT. Volumetric images of a zebrafish, stained by submersion in a 0.01% (w/v) solution of MB for 6 h, show accumulation of MB in the mesonephros, the primordial filtration organ.

  17. Fast speed MWIR imager for uncooled focal plane array

    NASA Astrophysics Data System (ADS)

    Lin, Liu

    2007-12-01

    Recent advances of uncooled detector technology especially the development of uncooled micro-bolometer array hold promise for us to develop low-cost and compact MWIR earth observation imager. For comparative lower radiometric performance of uncooled focal plane array, fast speed optical system operating in large spectral bands is compatible. In addition, in order to exhibit advantages over imagers based on cooled detector technology, the optical system should be as compact as possible which means fewer elements, smaller size and light weight. In this article, a high speed optical design meeting these requirement is provided with 100mm focal length, F/1 F number,+/-2.5°field of view woking in 3-5um wave band. The fast speed MWIR imager has properties mentioned as follows: First, the optical system utilizes a hybrid system including refractive and diffractive elements. Second, the optical system realizes athermalization in simple passive way through distributing power among the refractive elements. It can work under typical temperature scope from -20°C to 60°C for typical space application. Third, Because of high speed aperture, the design makes use of aspheric surface to correct spherical aberration and spherochromatism .Finally, we use Ge and Si material. instead of expensive ZnS material.

  18. Imaging Polarimetry With Polarization-Sensitive Focal Planes

    NASA Astrophysics Data System (ADS)

    Vorobiev, Dmitry; Ninkov, Z.

    2014-01-01

    We present a compact, lightweight, snapshot imaging polarimeter designed for operation in the near-infrared (NIR) and mid-infrared (MIR). Flux, polarization and spectral energy distribution are the fundamental measurements through which we infer properties of the sources of radiation such as intensity, temperature, chemical composition, emission mechanisms and structure. In recent decades, many scientific fields that utilize radiometry and spectroscopy have benefited from revolutionary improvements in instrumentation, for example, charge-coupled devices, hybridized infrared arrays, multi-object spectrometers and adaptive optics. Advances in polarimetric instrumentation have been more modest. Recently, the fabrication of microgrid polarizer arrays (MGPAs), facilitated the development of polarization-sensitive focal planes. These devices have inherent capability to measure the degree and angle of polarization across a scene (i.e., imaging polarimetry) instantaneously, without the need for multiple exposures and moving optics or multiple detectors. MGPA-based devices are compact, lightweight, and mechanically robust and perfectly suited for deployment on space-based and airborne platforms. We describe the design, operation and expected performance of MGPA-based imaging polarimeters and identify the applications for which these polarimeters are best suited.

  19. Color image encryption based on paired interpermuting planes

    NASA Astrophysics Data System (ADS)

    Zhang, Wei; Yu, Hai; Zhu, Zhi-liang

    2015-03-01

    A number of chaos-based image encryption algorithms have been proposed in recent years, and most of them employ confusion-diffusion architecture. This paper presents a new confusion scheme based on paired interpermuting planes. In the proposed new confusion operation, an "exchange and random access strategy" is employed to replace the traditional confusion operations. The efficiency of the proposed scheme was analyzed by evaluating its histogram distribution, its correlation coefficients, its ability to resist differential attacks, its ability to retain information (entropy analysis), its computational speed, and its ability to guarantee the security of its key scheme. Simulations have been carried out and the results confirmed the superior security and computing speed of our scheme compared to other comparable algorithms.

  20. Advances in Focal Plane Wavefront Estimation for Directly Imaging Exoplanets

    NASA Astrophysics Data System (ADS)

    Eldorado Riggs, A. J.; Kasdin, N. Jeremy; Groff, Tyler Dean

    2015-01-01

    To image cold exoplanets directly in visible light, an instrument on a telescope needs to suppress starlight by about 9 orders of magnitude at small separations from the star. A coronagraph changes the point spread function to create regions of high contrast where exoplanets or disks can be seen. Aberrations on the optics degrade the contrast by several orders of magnitude, so all high-contrast imaging systems incorporate one or more deformable mirrors (DMs) to recover regions of high contrast. With a coronagraphic instrument planned for the WFIRST-AFTA space telescope, there is a pressing need for faster, more robust estimation and control schemes for the DMs. Non-common path aberrations limit conventional phase conjugation schemes to medium star-to-planet contrast ratios of about 1e-6. High-contrast imaging requires estimation and control of both phase and amplitude in the same beam path as the science camera. Field estimation is a challenge since only intensity is measured; the most common approach, including that planned for WFIRST-AFTA, is to use DMs to create diversity, via pairs of small probe shapes, thereby allowing disambiguation of the electric field. Most implementations of DM Diversity require at least five images per electric field estimate and require narrowband measurements. This paper describes our new estimation algorithms that improve the speed (by using fewer images) and bandwidth of focal plane wavefront estimation. For narrowband estimation, we are testing nonlinear, recursive algorithms such as an iterative extended Kalman filter (IEKF) to use three images each iteration and build better, more robust estimates. We are also exploring the use of broadband estimation without the need for narrowband sub-filters and measurements. Here we present simulations of these algorithms with realistic noise and small signals to show how they might perform for WFIRST-AFTA. Once validated in simulations, we will test these algorithms experimentally in

  1. A CMOS Imager with Focal Plane Compression using Predictive Coding

    NASA Technical Reports Server (NTRS)

    Leon-Salas, Walter D.; Balkir, Sina; Sayood, Khalid; Schemm, Nathan; Hoffman, Michael W.

    2007-01-01

    This paper presents a CMOS image sensor with focal-plane compression. The design has a column-level architecture and it is based on predictive coding techniques for image decorrelation. The prediction operations are performed in the analog domain to avoid quantization noise and to decrease the area complexity of the circuit, The prediction residuals are quantized and encoded by a joint quantizer/coder circuit. To save area resources, the joint quantizerlcoder circuit exploits common circuitry between a single-slope analog-to-digital converter (ADC) and a Golomb-Rice entropy coder. This combination of ADC and encoder allows the integration of the entropy coder at the column level. A prototype chip was fabricated in a 0.35 pm CMOS process. The output of the chip is a compressed bit stream. The test chip occupies a silicon area of 2.60 mm x 5.96 mm which includes an 80 X 44 APS array. Tests of the fabricated chip demonstrate the validity of the design.

  2. Photoacoustic lifetime imaging of dissolved oxygen using methylene blue

    NASA Astrophysics Data System (ADS)

    Ashkenazi, Shai

    2010-07-01

    Measuring distribution of dissolved oxygen in biological tissue is of prime interest for cancer diagnosis, prognosis, and therapy optimization. Tumor hypoxia indicates poor prognosis and resistance to radiotherapy. Despite its major clinical significance, no current imaging modality provides direct imaging of tissue oxygen. We present preliminary results demonstrating the potential of photoacoustic lifetime imaging (PALI) for noninvasive, 3-D imaging of tissue oxygen. The technique is based on photoacoustic probing of the excited state lifetime of methylene blue (MB) dye. MB is an FDA-approved water soluble dye with a peak absorption at 660 nm. A double pulse laser system (pump probe) is used to excite the dye and probe its transient absorption by detecting photoacoustic emission. The relaxation rate of MB depends linearly on oxygen concentration. Our measurements show high photoacoustic signal contrast at a probe wavelength of 810 nm, where the excited state absorption is more than four times higher than the ground state absorption. Imaging of a simple phantom is demonstrated. We conclude by discussing possible implementations of the technique in clinical settings and combining it with photodynamic therapy (PDT) for real-time therapy monitoring.

  3. MAGPIS: A MULTI-ARRAY GALACTIC PLANE IMAGING SURVEY

    SciTech Connect

    Helfand, D J; Becker, R H; White, R L; Fallon, A; Tuttle, S

    2005-11-10

    We present the Multi-Array Galactic Plane Imaging Survey (MAGPIS), which maps portions of the first Galactic quadrant with an angular resolution, sensitivity and dynamic range that surpasses existing radio images of the Milky Way by more than an order of magnitude. The source detection threshold at 20 cm is in the range 1-2 mJy over the 85% of the survey region (5{sup o} < l < 32{sup o}, |b| < 0.8{sup o}) not covered by bright extended emission; the angular resolution is {approx} 6''. We catalog over 3000 discrete sources (diameters mostly < 30'') and present an atlas of {approx} 400 diffuse emission regions. New and archival data at 90 cm for the whole survey area are also presented. Comparison of our catalogs and images with the MSX mid-infrared data allow us to provide preliminary discrimination between thermal and non-thermal sources. We identify 49 high-probability supernova remnant candidates, increasing by a factor of seven the number of known remnants with diameters smaller than 50 in the survey region; several are pulsar wind nebula candidates and/or very small diameter remnants (D < 45''). We report the tentative identification of several hundred H II regions based on a comparison with the mid-IR data; they range in size from unresolved ultra-compact sources to large complexes of diffuse emission on scales of half a degree. In several of the latter regions, cospatial nonthermal emission illustrates the interplay between stellar death and birth. We comment briefly on plans for followup observations and our extension of the survey; when complemented by data from ongoing X-ray and mid-IR observations, we expect MAGPIS to provide an important contribution to our understanding of the birth and death of massive stars in the Milky Way.

  4. Statistical framework for the utilization of simultaneous pupil plane and focal plane telemetry for exoplanet imaging. I. Accounting for aberrations in multiple planes.

    PubMed

    Frazin, Richard A

    2016-04-01

    A new generation of telescopes with mirror diameters of 20 m or more, called extremely large telescopes (ELTs), has the potential to provide unprecedented imaging and spectroscopy of exoplanetary systems, if the difficulties in achieving the extremely high dynamic range required to differentiate the planetary signal from the star can be overcome to a sufficient degree. Fully utilizing the potential of ELTs for exoplanet imaging will likely require simultaneous and self-consistent determination of both the planetary image and the unknown aberrations in multiple planes of the optical system, using statistical inference based on the wavefront sensor and science camera data streams. This approach promises to overcome the most important systematic errors inherent in the various schemes based on differential imaging, such as angular differential imaging and spectral differential imaging. This paper is the first in a series on this subject, in which a formalism is established for the exoplanet imaging problem, setting the stage for the statistical inference methods to follow in the future. Every effort has been made to be rigorous and complete, so that validity of approximations to be made later can be assessed. Here, the polarimetric image is expressed in terms of aberrations in the various planes of a polarizing telescope with an adaptive optics system. Further, it is shown that current methods that utilize focal plane sensing to correct the speckle field, e.g., electric field conjugation, rely on the tacit assumption that aberrations on multiple optical surfaces can be represented as aberration on a single optical surface, ultimately limiting their potential effectiveness for ground-based astronomy.

  5. Plane Wave Imaging for ultrasonic non-destructive testing: Generalization to multimodal imaging.

    PubMed

    Le Jeune, Léonard; Robert, Sébastien; Lopez Villaverde, Eduardo; Prada, Claire

    2016-01-01

    This paper describes a new ultrasonic array imaging method for Non-Destructive Testing (NDT) which is derived from the medical Plane Wave Imaging (PWI) technique. The objective is to perform fast ultrasound imaging with high image quality. The approach is to transmit plane waves at several angles and to record the back-scattered signals with all the array elements. Focusing in receive is then achieved by coherent summations of the signals in every point of a region of interest. The medical PWI is generalized to immersion setups where water acts as a coupling medium and to multimodal (direct, half-skip modes) imaging in order to detect different types of defects (inclusions, porosities, cracks). This method is compared to the Total Focusing Method (TFM) which is the reference imaging technique in NDT. First, the two post-processing algorithms are described. Then experimental results with the array probe either in contact or in immersion are presented. A good agreement between the TFM and the PWI is observed, with three to ten times less transmissions required for the PWI.

  6. Observation angle and plane characterisation for ISAR imaging of LEO space objects

    NASA Astrophysics Data System (ADS)

    Chen, Jin; Fu, Tuo; Chen, Defeng; Gao, Meiguo

    2016-07-01

    For inverse synthetic aperture radar (ISAR) imaging of low Earth orbit (LEO) space objects, examining the variations in the image plane of the object over the entire visible arc period allows more direct characterisation of the variations in the object imaging. In this study, the ideal turntable model was extended to determine the observation geometry of near-circular LEO objects. Two approximations were applied to the observation model to calculate the image plane's normal and observation angles for near-circular orbit objects. One approximation treats the orbit of the space object as a standard arc relative to the Earth during the radar observation period, and the other omits the effect of the rotation of the Earth on the observations. First, the closed-form solution of the image plane normal in various attitude-stabilisation approaches was determined based on geometric models. The characteristics of the image plane and the observation angle of the near-circular orbit object were then analysed based on the common constraints of the radar line-of-sight (LOS). Subsequently, the variations in the image plane and the geometric constraints of the ISAR imaging were quantified. Based on the image plane's normal, the rotational angular velocity of the radar LOS was estimated. The cross-range direction of the ISAR image was then calibrated. Three-dimensional imaging was then reconstructed based on dual station interferometry. Finally, simulations were performed to verify the result of the three-dimensional interferometric reconstruction and to calculate the reconstruction's precision errors.

  7. Automatic Detection of Blue-White Veil and Related Structures in Dermoscopy Images

    PubMed Central

    Celebi, M. Emre; Iyatomi, Hitoshi; Stoecker, William V.; Moss, Randy H.; Rabinovitz, Harold S.; Argenziano, Giuseppe; Soyer, H. Peter

    2011-01-01

    Dermoscopy is a non-invasive skin imaging technique, which permits visualization of features of pigmented melanocytic neoplasms that are not discernable by examination with the naked eye. One of the most important features for the diagnosis of melanoma in dermoscopy images is the blue-white veil (irregular, structureless areas of confluent blue pigmentation with an overlying white “ground-glass” film). In this article, we present a machine learning approach to the detection of blue-white veil and related structures in dermoscopy images. The method involves contextual pixel classification using a decision tree classifier. The percentage of blue-white areas detected in a lesion combined with a simple shape descriptor yielded a sensitivity of 69.35% and a specificity of 89.97% on a set of 545 dermoscopy images. The sensitivity rises to 78.20% for detection of blue veil in those cases where it is a primary feature for melanoma recognition. PMID:18804955

  8. In-plane information from tapping mode AFM images

    NASA Astrophysics Data System (ADS)

    Marcus, Matthew

    2003-03-01

    Phase contrast in intermittent-contact atomic force microscopy is shown to reveal in-plane structural and mechanical properties of poly(diacetylene) monolayer films. This is surprising because measurements of in-plane properties typically require a contact mode of microscopy. Such measurements are possible because the tilt in the oscillating cantilever provides components of motion not just perpendicular to the surface, but also parallel to the sample surface. Lateral tip displacement is virtually universal in AFM, implying that any oscillating tip-AFM technique is sensitive to in-plane material properties. Although the tilt in the cantilever is small ( 10^o) it produces a component of motion that is 20% of the total tip displacement, and this motion accounts for 5-10% of dissipated energy through the tip-sample interaction[1]. The data is used in conjunction with a numerical model to extract in-plane material parameters. The effect of the cantilever tilt on phase measurements is directly verified through measurements on silicon samples tilted at a variety of angles with respect to the cantilever. The lateral tip displacement we make use of allows measurements of in-plane properties of soft samples such as polymer and biological samples. This work was done in collaboration with M. D'Amato, R.W. Carpick, and M.A. Eriksson, and was supported by the NSF CAREER and MRSEC programs and the Research Corporation. 1. M.S. Marcus, R.W. Carpick, D.Y. Sasaki, M.A. Eriksson, Phys. Rev. Lett. 88, 226103 (2002)

  9. Imaging photonic crystals using Fourier plane imaging and Fourier ptychographic microscopy techniques implemented with a computer controlled hemispherical digital condenser

    NASA Astrophysics Data System (ADS)

    Sen, Sanchari; Desai, Darshan B.; Alsubaie, Meznh H.; Zhelyeznyakov, Maksym V.; Molina, L.; Sarraf, Hamed Sari; Bernussi, Ayrton A.; Peralta, Luis Grave de

    2017-01-01

    Fourier plane imaging (FPIM) and Fourier ptychographic (FPM) microscopy techniques were used to image photonic crystals. A computer-controlled hemispherical digital condenser provided required sample illumination with variable inclination. Notable improvement in image resolution was obtained with both methods. However, it was determined that the FPM technique cannot surpass the Rayleigh resolution limit when imaging photonic crystals.

  10. Research on spatial-variant property of bistatic ISAR imaging plane of space target

    NASA Astrophysics Data System (ADS)

    Guo, Bao-Feng; Wang, Jun-Ling; Gao, Mei-Guo

    2015-04-01

    The imaging plane of inverse synthetic aperture radar (ISAR) is the projection plane of the target. When taking an image using the range-Doppler theory, the imaging plane may have a spatial-variant property, which causes the change of scatter’s projection position and results in migration through resolution cells. In this study, we focus on the spatial-variant property of the imaging plane of a three-axis-stabilized space target. The innovative contributions are as follows. 1) The target motion model in orbit is provided based on a two-body model. 2) The instantaneous imaging plane is determined by the method of vector analysis. 3) Three Euler angles are introduced to describe the spatial-variant property of the imaging plane, and the image quality is analyzed. The simulation results confirm the analysis of the spatial-variant property. The research in this study is significant for the selection of the imaging segment, and provides the evidence for the following data processing and compensation algorithm. Project supported by the National Natural Science Foundation of China (Grant No. 61401024), the Shanghai Aerospace Science and Technology Innovation Foundation, China (Grant No. SAST201240), and the Basic Research Foundation of Beijing Institute of Technology (Grant No. 20140542001).

  11. Vectorial point spread function and optical transfer function in oblique plane imaging.

    PubMed

    Kim, Jeongmin; Li, Tongcang; Wang, Yuan; Zhang, Xiang

    2014-05-05

    Oblique plane imaging, using remote focusing with a tilted mirror, enables direct two-dimensional (2D) imaging of any inclined plane of interest in three-dimensional (3D) specimens. It can image real-time dynamics of a living sample that changes rapidly or evolves its structure along arbitrary orientations. It also allows direct observations of any tilted target plane in an object of which orientational information is inaccessible during sample preparation. In this work, we study the optical resolution of this innovative wide-field imaging method. Using the vectorial diffraction theory, we formulate the vectorial point spread function (PSF) of direct oblique plane imaging. The anisotropic lateral resolving power caused by light clipping from the tilted mirror is theoretically analyzed for all oblique angles. We show that the 2D PSF in oblique plane imaging is conceptually different from the inclined 2D slice of the 3D PSF in conventional lateral imaging. Vectorial optical transfer function (OTF) of oblique plane imaging is also calculated by the fast Fourier transform (FFT) method to study effects of oblique angles on frequency responses.

  12. Blue laser imaging endoscopy system for the early detection and characterization of colorectal lesions: a guide for the endoscopist

    PubMed Central

    Togashi, Kazutomo; Nemoto, Daiki; Utano, Kenichi; Isohata, Noriyuki; Kumamoto, Kensuke; Endo, Shungo; Lefor, Alan K.

    2016-01-01

    Blue laser imaging is a new system for image-enhanced endoscopy using laser light. Blue laser imaging utilizes two monochromatic lasers (410 and 450 nm) instead of xenon light. A 410 nm laser visualizes vascular microarchitecture, similar to narrow band imaging, and a 450 nm laser provides white light by excitation. According to three recently published reports, the diagnostic ability of polyp characterization using blue laser imaging compares favorably with narrow band imaging. No published data are available to date regarding polyp detection with blue laser imaging. However, blue laser imaging has the possibility to increase the detection of colorectal polyps by depicting brighter and clearer endoscopic images, even at a distant view, compared with first-generation image-enhanced endoscopy. A clinical trial to compare the detection between blue laser imaging and xenon light is warranted. PMID:26770267

  13. Blue laser imaging endoscopy system for the early detection and characterization of colorectal lesions: a guide for the endoscopist.

    PubMed

    Togashi, Kazutomo; Nemoto, Daiki; Utano, Kenichi; Isohata, Noriyuki; Kumamoto, Kensuke; Endo, Shungo; Lefor, Alan K

    2016-01-01

    Blue laser imaging is a new system for image-enhanced endoscopy using laser light. Blue laser imaging utilizes two monochromatic lasers (410 and 450 nm) instead of xenon light. A 410 nm laser visualizes vascular microarchitecture, similar to narrow band imaging, and a 450 nm laser provides white light by excitation. According to three recently published reports, the diagnostic ability of polyp characterization using blue laser imaging compares favorably with narrow band imaging. No published data are available to date regarding polyp detection with blue laser imaging. However, blue laser imaging has the possibility to increase the detection of colorectal polyps by depicting brighter and clearer endoscopic images, even at a distant view, compared with first-generation image-enhanced endoscopy. A clinical trial to compare the detection between blue laser imaging and xenon light is warranted.

  14. The application of digital image plane holography technology to identify Chinese herbal medicine

    NASA Astrophysics Data System (ADS)

    Wang, Huaying; Guo, Zhongjia; Liao, Wei; Zhang, Zhihui

    2012-03-01

    In this paper, the imaging technology of digital image plane holography to identify the Chinese herbal medicine is studied. The optical experiment system of digital image plane holography which is the special case of pre-magnification digital holography was built. In the record system, one is an object light by using plane waves which illuminates the object, and the other one is recording hologram by using spherical light wave as reference light. There is a Micro objective lens behind the object. The second phase factor which caus ed by the Micro objective lens can be eliminated by choosing the proper position of the reference point source when digital image plane holography is recorded by spherical light. In this experiment, we use the Lygodium cells and Onion cells as the object. The experiment results with Lygodium cells and Onion cells show that digital image plane holography avoid the process of finding recording distance by using auto-focusing approach, and the phase information of the object can be reconstructed more accurately. The digital image plane holography is applied to the microscopic imaging of cells more effectively, and it is suit to apply for the identify of Chinese Herbal Medicine. And it promotes the application of digital holographic in practice.

  15. The application of digital image plane holography technology to identify Chinese herbal medicine

    NASA Astrophysics Data System (ADS)

    Wang, Huaying; Guo, Zhongjia; Liao, Wei; Zhang, Zhihui

    2011-11-01

    In this paper, the imaging technology of digital image plane holography to identify the Chinese herbal medicine is studied. The optical experiment system of digital image plane holography which is the special case of pre-magnification digital holography was built. In the record system, one is an object light by using plane waves which illuminates the object, and the other one is recording hologram by using spherical light wave as reference light. There is a Micro objective lens behind the object. The second phase factor which caus ed by the Micro objective lens can be eliminated by choosing the proper position of the reference point source when digital image plane holography is recorded by spherical light. In this experiment, we use the Lygodium cells and Onion cells as the object. The experiment results with Lygodium cells and Onion cells show that digital image plane holography avoid the process of finding recording distance by using auto-focusing approach, and the phase information of the object can be reconstructed more accurately. The digital image plane holography is applied to the microscopic imaging of cells more effectively, and it is suit to apply for the identify of Chinese Herbal Medicine. And it promotes the application of digital holographic in practice.

  16. An automatic recognition and parameter extraction method for structural planes in borehole image

    NASA Astrophysics Data System (ADS)

    Wang, Chuanying; Zou, Xianjian; Han, Zengqiang; Wang, Yiteng; Wang, Jinchao

    2016-12-01

    As a breakthrough in borehole imaging technology, digital panoramic borehole camera technology has been widely employed. The high-resolution panoramic borehole images can accurately reproduce the geometric features of structural planes. However, the detection of these features is usually done manually, which is both time-consuming and introduces human errors. To solve this problem, this paper presents a method for the automatic recognition and parameter extraction of borehole geometric features of camera images. In this method, the image's gray and gradient level, and also their projection on the depth axis are used to identify the locations of structural planes. Afterwards, iterative matching is employed by using a template of sinusoidal function to search for structural planes in the identified image blocks. Finally, optimal sine curves are selected as the feature curves of structural planes, and their related parameters are converted into structural plane parameters required for engineering, such as their positions, dip directions, dip angles and fracture widths. The method can automatically identify all of structural planes throughout the whole borehole camera image in a continuous and rapid manner, and obtain the corresponding structural parameters. It has proven highly reliable, accurate and efficient.

  17. High contrast imaging through adaptive transmittance control in the focal plane

    NASA Astrophysics Data System (ADS)

    Dhadwal, Harbans S.; Rastegar, Jahangir; Feng, Dake

    2016-05-01

    High contrast imaging, in the presence of a bright background, is a challenging problem encountered in diverse applications ranging from the daily chore of driving into a sun-drenched scene to in vivo use of biomedical imaging in various types of keyhole surgeries. Imaging in the presence of bright sources saturates the vision system, resulting in loss of scene fidelity, corresponding to low image contrast and reduced resolution. The problem is exacerbated in retro-reflective imaging systems where the light sources illuminating the object are unavoidably strong, typically masking the object features. This manuscript presents a novel theoretical framework, based on nonlinear analysis and adaptive focal plane transmittance, to selectively remove object domain sources of background light from the image plane, resulting in local and global increases in image contrast. The background signal can either be of a global specular nature, giving rise to parallel illumination from the entire object surface or can be represented by a mosaic of randomly orientated, small specular surfaces. The latter is more representative of real world practical imaging systems. Thus, the background signal comprises of groups of oblique rays corresponding to distributions of the mosaic surfaces. Through the imaging system, light from group of like surfaces, converges to a localized spot in the focal plane of the lens and then diverges to cast a localized bright spot in the image plane. Thus, transmittance of a spatial light modulator, positioned in the focal plane, can be adaptively controlled to block a particular source of background light. Consequently, the image plane intensity is entirely due to the object features. Experimental image data is presented to verify the efficacy of the methodology.

  18. Support plane method applied to ground objects recognition using modelled SAR images

    NASA Astrophysics Data System (ADS)

    Zherdev, Denis A.; Fursov, Vladimir A.

    2015-09-01

    In this study, the object recognition problem was solved using support plane method. The modelled SAR images were used as features vectors in the recognition algorithm. Radar signal backscattering of objects in different observing poses is presented in SAR images. For real time simulation, we used simple mixture model of Lambertian-specular reflectivity. To this end, an algorithm of ray tracing is extended for simulating SAR images of 3D man-made models. The suggested algorithm of support plane is very effective in objects recognition using SAR images and RCS diagrams.

  19. Ultrasound plane-wave imaging with delay multiply and sum beamforming and coherent compounding.

    PubMed

    Matrone, Giulia; Savoia, Alessandro S; Caliano, Giosue; Magenes, Giovanni

    2016-08-01

    Improving the frame rate is an important aspect in medical ultrasound imaging, particularly in 3D/4D cardiac applications. However, an accurate trade-off between the higher frame rate and image contrast and resolution should be performed. Plane-Wave Imaging (PWI) can potentially achieve frame rates in the order of 10 kHz, as it uses a single unfocused plane wave (and thus a single transmit event) to acquire the image of the entire region of interest. The lack of transmit focusing however causes a significant drop of image quality, which can be restored by coherently compounding several tilted plane-wave frames, at the expense of the frame rate. PWI together with the use of a beamforming algorithm able to achieve a higher image contrast resolution, such as the Delay Multiply And Sum (DMAS), could thus allow to improve image quality achieving a high frame rate at the same time. This paper presents the first simulation results obtained by employing DMAS beamforming and PWI with different transmission angles and coherent compounding. The simulated Point Spread Function (PSF) and cyst-phantom images show that DMAS makes it possible to achieve a high image quality with a reduced number of compounded frames compared to standard Delay And Sum (DAS), and hence it can be used to improve the contrast and resolution of plane-wave images still achieving a very high frame rate.

  20. Focal plane resolution and overlapped array time delay and integrate imaging

    NASA Astrophysics Data System (ADS)

    Grycewicz, Thomas J.; Cota, Stephen A.; Lomheim, Terrence S.; Kalman, Linda S.

    2010-06-01

    In this paper we model sub-pixel image registration for a generic earth-observing satellite system with a focal plane using two offset time delay and integrate (TDI) arrays in the focal plane to improve the achievable ground resolution over the resolution achievable with a single array. The modeling process starts with a high-resolution image as ground truth. The Parameterized Image Chain Analysis & Simulation Software (PICASSO) modeling tool is used to degrade the images to match the optical transfer function, sampling, and noise characteristics of the target system. The model outputs a pair of images with a separation close to the nominal half-pixel separation between the overlapped arrays. A registration estimation algorithm is used to measure the offset for image reconstruction. The two images are aligned and summed on a grid with twice the capture resolution. We compare the resolution in images between the inputs before overlap, the reconstructed image, and a simulation for the image which would have been captured on a focal plane with twice the resolution. We find the performance to always be better than the lower resolution baseline, and to approach the performance of the high-resolution array in the ideal case. We show that the overlapped array imager significantly outperforms both the conventional high- and low-resolution imagers in conditions with high image smear.

  1. Bit-plane-channelized hotelling observer for predicting task performance using lossy-compressed images

    NASA Astrophysics Data System (ADS)

    Schmanske, Brian M.; Loew, Murray H.

    2003-05-01

    A technique for assessing the impact of lossy wavelet-based image compression on signal detection tasks is presented. A medical image"s value is based on its ability to support clinical decisions such as detecting and diagnosing abnormalities. Image quality of compressed images is, however, often stated in terms of mathematical metrics such as mean square error. The presented technique provides a more suitable measure of image degradation by building on the channelized Hotelling observer model, which has been shown to predict human performance of signal detection tasks in noise-limited images. The technique first decomposes an image into its constituent wavelet subband coefficient bit-planes. Channel responses for the individual subband bit-planes are computed, combined,and processed with a Hotelling observer model to provide a measure of signal detectability versus compression ratio. This allows a user to determine how much compression can be tolerated before signal detectability drops below a certain threshold.

  2. Single-plane versus three-plane methods for relative range error evaluation of medium-range 3D imaging systems

    NASA Astrophysics Data System (ADS)

    MacKinnon, David K.; Cournoyer, Luc; Beraldin, J.-Angelo

    2015-05-01

    Within the context of the ASTM E57 working group WK12373, we compare the two methods that had been initially proposed for calculating the relative range error of medium-range (2 m to 150 m) optical non-contact 3D imaging systems: the first is based on a single plane (single-plane assembly) and the second on an assembly of three mutually non-orthogonal planes (three-plane assembly). Both methods are evaluated for their utility in generating a metric to quantify the relative range error of medium-range optical non-contact 3D imaging systems. We conclude that the three-plane assembly is comparable to the single-plane assembly with regard to quantification of relative range error while eliminating the requirement to isolate the edges of the target plate face.

  3. Horizontal Long Axis Imaging Plane for Evaluation of Right Ventricular Function on Cardiac Magnetic Resonance Imaging

    PubMed Central

    Chaturvedi, Abhishek; Whitnah, Joseph; Maki, Jeffrey H; Baran, Timothy; Mitsumori, Lee M

    2016-01-01

    Purpose: The purpose of this study was to evaluate a horizontal long axis (HLA) magnetic resonance imaging (MRI) plane aligned to the long axis of the right ventricular (RV) cavity for functional analysis by comparing the measurement variability and time required for the analysis with that using a short-axis (SAX) image orientation. Materials and Methods: Thirty-four cardiac MRI exams with cine balanced steady-state free precession image stacks in both the SAX and the HLA of the RV (RHLA) were evaluated. Two reviewers independently traced RV endocardial borders on each image of the cine stacks. The time required to complete each set of traces was recorded, and the RV end-diastolic volume, end-systolic volume, and ejection fraction were calculated. Analysis times and RV measurements were compared between the two orientations. Results: Analysis time for each reviewer was significantly shorter for the RHLA stack (reviewer 1 = 6.4 ± 1.8 min, reviewer 2 = 6.0 ± 3.3 min) than for the SAX stack (7.5 ± 2.1 and 6.9 ± 3.6 min, respectively; P < 0.002). Bland–Altman analysis revealed lower mean differences, limits of agreement, and coefficients of variation for RV measurements obtained with the RHLA stack. Conclusions: RV functional analysis using a RHLA stack resulted in shorter analysis times and lower measurement variability than for a SAX stack orientation. PMID:28123842

  4. Using Satellite Images for Wireless Network Planing in Baku City

    NASA Astrophysics Data System (ADS)

    Gojamanov, M.; Ismayilov, J.

    2013-04-01

    It is a well known fact that the Information-Telecommunication and Space research technologies are the fields getting much more benefits from the achievements of the scientific and technical progress. In many cases, these areas supporting each other have improved the conditions for their further development. For instance, the intensive development in the field of the mobile communication has caused the rapid progress of the Space research technologies and vice versa.Today it is impossible to solve one of the most important tasks of the mobile communication as Radio Frecance planning without the 2D and 3D digital maps. The compiling of such maps is much more efficient by means of the space images. Because the quality of the space images has been improved and developed, especially at the both spectral and spatial resolution points. It has been possible to to use 8 Band images with the spatial resolution of 50 sm. At present, in relation to the function 3G of mobile communications one of the main issues facing mobile operator companies is a high-precision 3D digital maps. It should be noted that the number of mobile phone users in the Republic of Azerbaijan went forward other Community of Independent States Countries. Of course, using of aerial images for 3D mapping would be optimal. However, depending on a number of technical and administrative problems aerial photography cannot be used. Therefore, the experience of many countries shows that it will be more effective to use the space images with the higher resolution for these issues. Concerning the fact that the mobile communication within the city of Baku has included 3G function there were ordered stereo images wih the spatial resolution of 50 cm for the 150 sq.km territory occupying the central part of the city in order to compile 3D digital maps. The images collected from the WorldView-2 satellite are 4-Band Bundle(Pan+MS1) stereo images. Such kind of imagery enable to automatically classificate some required

  5. A Digital Camcorder Image Stabilizer Based on Gray Coded Bit-Plane Block Matching

    DTIC Science & Technology

    2000-07-01

    Matching", IEEE 1998. [9] Sung- Hee Lee, Seung -Won Jeon, Eui-Sung Kang and Sung-Jea Ko, "Fast Digital Stabilizer based on Gray Coded Bit- Plane Matching...Jea Ko and Sung- Hee Lee adopted bit-plane or gray-coded bit-plane block matching to greatly reduce the computational complexity. However, their...Yong Chul Park, and Dong Wook Kim , "An Adaptive Motion Decision System for Digital Image Stabilizer Based on Edge Pattern Matching", IEEE Trans. on

  6. Effect of novel bright image enhanced endoscopy using blue laser imaging (BLI)

    PubMed Central

    Kaneko, Kazuhiro; Oono, Yasuhiro; Yano, Tomonori; Ikematsu, Hiroaki; Odagaki, Tomoyuki; Yoda, Yusuke; Yagishita, Atsushi; Sato, Akihiro; Nomura, Shogo

    2014-01-01

    Background and study aims: The novel method of image-enhanced endoscopy (IEE) named blue laser imaging (BLI) can enhance the contrast of surface vessels using lasers for light illumination. BLI has two IEE modes: high contrast mode (BLI-contrast) for use with magnification, and bright mode (BLI-bright), which achieves a brighter image than BLI-contrast and yet maintains the enhanced visualization of vascular contrast that is expected for the detection of tumors from a far field of view. The aim of this study is to clarify the effect of BLI-bright with a far field of view compared to BLI-contrast and commonly available narrow-band imaging (NBI). Patients and methods: Patients with neoplasia, including early cancer in the pharynx, esophagus, stomach, or colorectum, were recruited and underwent tandem endoscopy with BLI and NBI systems. Six sets of images of the lesions were captured with a changing observable distance from 3 to 40 mm. Individual sets of images taken from various observable distances were assessed for visibility among BLI-bright, BLI-contrast, and NBI modes. The brightness and contrast of these images were also analyzed quantitatively. Results: Of 51 patients, 39 were assessed. Image analysis indicated that only BLI-bright maintained adequate brightness and contrast up to 40 mm and had significantly longer observable distances compared to the other methods. Furthermore, BLI-bright enhanced the visualization of serious lesions infiltrating into deeper layers, such as esophageal lamina propria or gastric submucosal cancers. Conclusions: BLI-bright will be a helpful tool for the far-field view with IEE in organs with wider internal spaces such as the stomach. PMID:26135095

  7. NESP: Nonlinear enhancement and selection of plane for optimal segmentation and recognition of scene word images

    NASA Astrophysics Data System (ADS)

    Kumar, Deepak; Anil Prasad, M. N.; Ramakrishnan, A. G.

    2013-01-01

    In this paper, we report a breakthrough result on the difficult task of segmentation and recognition of coloured text from the word image dataset of ICDAR robust reading competition challenge 2: reading text in scene images. We split the word image into individual colour, gray and lightness planes and enhance the contrast of each of these planes independently by a power-law transform. The discrimination factor of each plane is computed as the maximum between-class variance used in Otsu thresholding. The plane that has maximum discrimination factor is selected for segmentation. The trial version of Omnipage OCR is then used on the binarized words for recognition. Our recognition results on ICDAR 2011 and ICDAR 2003 word datasets are compared with those reported in the literature. As baseline, the images binarized by simple global and local thresholding techniques were also recognized. The word recognition rate obtained by our non-linear enhancement and selection of plance method is 72.8% and 66.2% for ICDAR 2011 and 2003 word datasets, respectively. We have created ground-truth for each image at the pixel level to benchmark these datasets using a toolkit developed by us. The recognition rate of benchmarked images is 86.7% and 83.9% for ICDAR 2011 and 2003 datasets, respectively.

  8. The Gini Coefficient as a Morphological Measurement of Strongly Lensed Galaxies in the Image Plane

    NASA Astrophysics Data System (ADS)

    Florian, Michael K.; Li, Nan; Gladders, Michael D.

    2016-12-01

    Characterization of the morphology of strongly lensed galaxies is challenging because images of such galaxies are typically highly distorted. Lens modeling and source plane reconstruction is one approach that can provide reasonably undistorted images from which morphological measurements can be made, though at the expense of a highly spatially variable telescope point-spread function (PSF) when mapped back to the source plane. Unfortunately, modeling the lensing mass is a time- and resource-intensive process, and in many cases there are too few constraints to precisely model the lensing mass. If, however, useful morphological measurements could be made in the image plane rather than the source plane, it would bypass this issue and obviate the need for a source reconstruction process for some applications. We examine the use of the Gini coefficient as one such measurement. Because it depends on the cumulative distribution of the light of a galaxy, but not the relative spatial positions, the fact that surface brightness is conserved by lensing means that the Gini coefficient may be well preserved by strong gravitational lensing. Through simulations, we test the extent to which the Gini coefficient is conserved, including by effects due to PSF convolution and pixelization, to determine whether it is invariant enough under lensing to be used as a measurement of galaxy morphology that can be made in the image plane.

  9. Speckle-based volume holographic microscopy for optically sectioned multi-plane fluorescent imaging.

    PubMed

    Chen, Hsi-Hsun; Singh, Vijay Raj; Luo, Yuan

    2015-03-23

    Structured illumination microscopy has been widely used to reconstruct optically sectioned fluorescence images in wide-field fashion; however, it still requires axial scanning to obtain multiple depth information of a volumetric sample. In this paper, a new imaging scheme, called speckle-based volume holographic microscopy system, is presented. The proposed system incorporates volumetric speckle illumination and multiplexed volume holographic gratings to acquire multi-plane images with optical sectioning capability, without any axial scanning. We present the design, implementation, and experimental image data demonstrating the proposed system's ability to simultaneously obtain wide-field, optically sectioned, and multi-depth resolved images of fluorescently labeled microspheres and tissue structures.

  10. Modeled and measured image-plane polychromatic speckle contrast

    NASA Astrophysics Data System (ADS)

    Van Zandt, Noah R.; McCrae, Jack E.; Fiorino, Steven T.

    2016-02-01

    The statistical properties of speckle relevant to short- to medium-range (tactical) active tracking involving polychromatic illumination are investigated. A numerical model is developed to allow rapid simulation of speckled images including the speckle contrast reduction effects of illuminator bandwidth, surface slope, and roughness, and the polarization properties of both the source and the reflection. Regarding surface slope (relative orientation of the surface normal and illumination/observation directions), Huntley's theory for speckle contrast, which employs geometrical approximations to decrease computation time, is modified to increase accuracy by incorporation of a geometrical correction factor and better treatment of roughness and polarization. The resulting model shows excellent agreement with more exact theory over a wide range. An experiment is conducted to validate both the numerical model developed here and existing theory. A diode laser source with coherence length of 259±7 μm is reflected off of a silver-coated diffuse surface. Speckle data are gathered for 16 surface slope angles corresponding to speckle contrast between about 0.55 and 1. Taking the measured data as truth, both equations show error mean and standard deviation of less than 3%. Thus, the theory is validated over the range of this experiment.

  11. Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

    PubMed Central

    Fu, Qinyi; Martin, Benjamin L.; Matus, David Q.; Gao, Liang

    2016-01-01

    Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos. PMID:27004937

  12. Imaging the in-plane magnetization in a Co microstructure by Fourier transform holography.

    PubMed

    Tieg, C; Frömter, R; Stickler, D; Hankemeier, S; Kobs, A; Streit-Nierobisch, S; Gutt, C; Grübel, G; Oepen, H P

    2010-12-20

    We report on experiments using Fourier transform holography to image the in-plane magnetization of a magnetic microstructure. Magnetic sensitivity is achieved via the x-ray magnetic circular dichroism effect by recording holograms in transmission at off-normal incidence. The reference beam is defined by a narrow hole milled at an inclined angle into the opaque mask. We present magnetic domain images of an in-plane magnetized cobalt element with a size of 2 μm × 2 μm× 20 nm. The domain pattern shows a multi-vortex state that deviates from the simple Landau ground state.

  13. Mid-callosal plane determination using preferred directions from diffusion tensor images

    NASA Astrophysics Data System (ADS)

    Costa, André L.; Rittner, Letícia; Lotufo, Roberto A.; Appenzeller, Simone

    2015-03-01

    The corpus callosum is the major brain structure responsible for inter{hemispheric communication between neurons. Many studies seek to relate corpus callosum attributes to patient characteristics, cerebral diseases and psychological disorders. Most of those studies rely on 2D analysis of the corpus callosum in the mid-sagittal plane. However, it is common to find conflicting results among studies, once many ignore methodological issues and define the mid-sagittal plane based on precary or invalid criteria with respect to the corpus callosum. In this work we propose a novel method to determine the mid-callosal plane using the corpus callosum internal preferred diffusion directions obtained from diffusion tensor images. This plane is analogous to the mid-sagittal plane, but intended to serve exclusively as the corpus callosum reference. Our method elucidates the great potential the directional information of the corpus callosum fibers have to indicate its own referential. Results from experiments with five image pairs from distinct subjects, obtained under the same conditions, demonstrate the method effectiveness to find the corpus callosum symmetric axis relative to the axial plane.

  14. Determining the imaging plane of a retinal capillary layer in adaptive optical imaging

    NASA Astrophysics Data System (ADS)

    Yang, Le-Bao; Hu, Li-Fa; Li, Da-Yu; Cao, Zhao-Liang; Mu, Quan-Quan; Ma, Ji; Xuan, Li

    2016-09-01

    Even in the early stage, endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm. However, the fundus cameras used in clinic diagnosis can only obtain images of vessels larger than 20 μm in diameter. The human retina is a thin and multiple layer tissue, and the layer of capillaries less than 10 μm in diameter only exists in the inner nuclear layer. The layer thickness of capillaries less than 10 μm in diameter is about 40 μm and the distance range to rod&cone cell surface is tens of micrometers, which varies from person to person. Therefore, determining reasonable capillary layer (CL) position in different human eyes is very difficult. In this paper, we propose a method to determine the position of retinal CL based on the rod&cone cell layer. The public positions of CL are recognized with 15 subjects from 40 to 59 years old, and the imaging planes of CL are calculated by the effective focal length of the human eye. High resolution retinal capillary imaging results obtained from 17 subjects with a liquid crystal adaptive optics system (LCAOS) validate our method. All of the subjects’ CLs have public positions from 127 μm to 147 μm from the rod&cone cell layer, which is influenced by the depth of focus. Project supported by the National Natural Science Foundation of China (Grant Nos. 11174274, 11174279, 61205021, 11204299, 61475152, and 61405194).

  15. Supplemental blue LED lighting array to improve the signal quality in hyperspectral imaging of plants.

    PubMed

    Mahlein, Anne-Katrin; Hammersley, Simon; Oerke, Erich-Christian; Dehne, Heinz-Wilhelm; Goldbach, Heiner; Grieve, Bruce

    2015-06-01

    Hyperspectral imaging systems used in plant science or agriculture often have suboptimal signal-to-noise ratio in the blue region (400-500 nm) of the electromagnetic spectrum. Typically there are two principal reasons for this effect, the low sensitivity of the imaging sensor and the low amount of light available from the illuminating source. In plant science, the blue region contains relevant information about the physiology and the health status of a plant. We report on the improvement in sensitivity of a hyperspectral imaging system in the blue region of the spectrum by using supplemental illumination provided by an array of high brightness light emitting diodes (LEDs) with an emission peak at 470 nm.

  16. Supplemental Blue LED Lighting Array to Improve the Signal Quality in Hyperspectral Imaging of Plants

    PubMed Central

    Mahlein, Anne-Katrin; Hammersley, Simon; Oerke, Erich-Christian; Dehne, Heinz-Wilhelm; Goldbach, Heiner; Grieve, Bruce

    2015-01-01

    Hyperspectral imaging systems used in plant science or agriculture often have suboptimal signal-to-noise ratio in the blue region (400–500 nm) of the electromagnetic spectrum. Typically there are two principal reasons for this effect, the low sensitivity of the imaging sensor and the low amount of light available from the illuminating source. In plant science, the blue region contains relevant information about the physiology and the health status of a plant. We report on the improvement in sensitivity of a hyperspectral imaging system in the blue region of the spectrum by using supplemental illumination provided by an array of high brightness light emitting diodes (LEDs) with an emission peak at 470 nm. PMID:26039423

  17. Spread-Spectrum Beamforming and Clutter Filtering for Plane-Wave Color Doppler Imaging.

    PubMed

    Mansour, Omar; Poepping, Tamie L; Lacefield, James C

    2016-07-21

    Plane-wave imaging is desirable for its ability to achieve high frame rates, allowing the capture of fast dynamic events and continuous Doppler data. In most implementations of plane-wave imaging, multiple low-resolution images from different plane wave tilt angles are compounded to form a single high-resolution image, thereby reducing the frame rate. Compounding improves the lateral beam profile in the high-resolution image, but it also acts as a low-pass filter in slow time that causes attenuation and aliasing of signals with high Doppler shifts. This paper introduces a spread-spectrum color Doppler imaging method that produces high-resolution images without the use of compounding, thereby eliminating the tradeoff between beam quality, maximum unaliased Doppler frequency, and frame rate. The method uses a long, random sequence of transmit angles rather than a linear sweep of plane wave directions. The random angle sequence randomizes the phase of off-focus (clutter) signals, thereby spreading the clutter power in the Doppler spectrum, while keeping the spectrum of the in-focus signal intact. The ensemble of randomly tilted low-resolution frames also acts as the Doppler ensemble, so it can be much longer than a conventional linear sweep, thereby improving beam formation while also making the slow-time Doppler sampling frequency equal to the pulse repetition frequency. Experiments performed using a carotid artery phantom with constant flow demonstrate that the spread-spectrum method more accurately measures the parabolic flow profile of the vessel and outperforms conventional plane-wave Doppler in both contrast resolution and estimation of high flow velocities. The spread-spectrum method is expected to be valuable for Doppler applications that require measurement of high velocities at high frame rates.

  18. Optical simulation of in-plane-switching blue phase liquid crystal display using the finite-difference time-domain method

    NASA Astrophysics Data System (ADS)

    Dou, Hu; Ma, Hongmei; Sun, Yu-Bao

    2016-09-01

    The finite-difference time-domain method is used to simulate the optical characteristics of an in-plane switching blue phase liquid crystal display. Compared with the matrix optic methods and the refractive method, the finite-difference time-domain method, which is used to directly solve Maxwell’s equations, can consider the lateral variation of the refractive index and obtain an accurate convergence effect. The simulation results show that e-rays and o-rays bend in different directions when the in-plane switching blue phase liquid crystal display is driven by the operating voltage. The finite-difference time-domain method should be used when the distribution of the liquid crystal in the liquid crystal display has a large lateral change. Project supported by the National Natural Science Foundation of China (Grant Nos. 11304074, 61475042, and 11274088), the Natural Science Foundation of Hebei Province, China (Grant Nos. A2015202320 and GCC2014048), and the Key Subject Construction Project of Hebei Province University, China.

  19. An automated image-based tool for pupil plane characterization of EUVL tools

    NASA Astrophysics Data System (ADS)

    Levinson, Zac; Smith, Jack S.; Fenger, Germain; Smith, Bruce W.

    2016-03-01

    Pupil plane characterization will play a critical role in image process optimization for EUV lithography (EUVL), as it has for several lithography generations. In EUVL systems there is additional importance placed on understanding the ways that thermally-induced system drift affect pupil variation during operation. In-situ full pupil characterization is therefore essential for these tools. To this end we have developed Quick Inverse Pupil (QUIP)—a software suite developed for rapid characterization of pupil plane behavior based on images formed by that system. The software consists of three main components: 1) an image viewer, 2) the model builder, and 3) the wavefront analyzer. The image viewer analyzes CDSEM micrographs or actinic mask micrographs to measure either CDs or through-focus intensity volumes. The software is capable of rotation correction and image registration with subpixel accuracy. The second component pre-builds a model for a particular imaging system to enable rapid pupil characterization. Finally, the third component analyzes the results from the image viewer and uses the optional pre-built model for inverse solutions of pupil plane behavior. Both pupil amplitude and phase variation can be extracted using this software. Inverse solutions are obtained through a model based algorithm which is built on top of commercial rigorous full-vector simulation software.

  20. Three-dimensional imaging of the uterus: The value of the coronal plane

    PubMed Central

    Wong, Lufee; White, Nikki; Ramkrishna, Jayshree; Júnior, Edward Araujo; Meagher, Simon; Costa, Fabricio Da Silva

    2015-01-01

    Advent in three-dimensional (3D) imaging technology has seen 3D ultrasound establish itself as a useful adjunct complementary to traditional two-dimensional imaging of the female pelvis. This advantage largely arises from its ability to reconstruct the coronal plane of the uterus, which allows further delineation of many gynecological disorders. 3D imaging of the uterus is now the preferred imaging modality for assessing congenital uterine anomalies and intrauterine device localization. Newer indications include the diagnosis of adenomyosis. It can also add invaluable information to delineate other endometrial and myometrial pathology such as fibroids and endometrial polyps. PMID:26753063

  1. Present and future status of flexible spectral imaging color enhancement and blue laser imaging technology.

    PubMed

    Osawa, Hiroyuki; Yamamoto, Hironori

    2014-01-01

    The usefulness of flexible spectral imaging color enhancement (FICE) has been reported for evaluating the esophagus, stomach, and small and large intestine. Higher contrast is shown between cancer and the surrounding mucosa in the esophagus and stomach and may facilitate the detection of gastric cancers missed by white light imaging alone. The surface patterns of gastric mucosa are clearly visualized in non-malignant areas but are irregular and blurred in malignant areas, leading to clear demarcation. Capsule endoscopy with FICE detects angiodysplasia and erosions of the small intestine. The surface and vascular pattern with FICE is useful for the differential diagnosis of colorectal polyps. However, FICE remains somewhat poor at visualizing mucosal microvasculature on a tumor surface. Narrow-band imaging (NBI) is dark in observing whole gastric mucosa and poor at visualizing mucosal microstructure. Blue laser imaging (BLI) has the potential to resolve these limitations. Narrow-band laser light combined with white light shows irregular microvessels on both differentiated and undifferentiated gastric cancer similar to those using NBI. In addition, irregular surface patterns including minute white zones are clearly seen on the uneven surface of differentiated lesions, resulting in exclusion of undifferentiated lesions. Using both distant and close-up views, a high contrast between green intestinal metaplasia and brown gastric cancer may lead to early detection of gastric cancers and determination of a demarcation line. BLI produces high-contrast images in esophageal cancer with clear vision of intrapapillary capillary loops and also predicts the histopathological diagnosis and depth of invasion in colorectal neoplasms.

  2. Chemical imaging of cotton fibers using an infrared microscope and a focal-plane array detector

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this presentation, the chemical imaging of cotton fibers with an infrared microscope and a Focal-Plane Array (FPA) detector will be discussed. Infrared spectroscopy can provide us with information on the structure and quality of cotton fibers. In addition, FPA detectors allow for simultaneous spe...

  3. Robust midsagittal plane extraction from normal and pathological 3-D neuroradiology images.

    PubMed

    Liu, Y; Collins, R T; Rothfus, W E

    2001-03-01

    This paper focuses on extracting the ideal midsagittal plane (iMSP) from three-dimensional (3-D) normal and pathological neuroimages. The main challenges in this work are the structural asymmetry that may exist in pathological brains, and the anisotropic, unevenly sampled image data that is common in clinical practice. We present an edge-based, cross-correlation approach that decomposes the plane fitting problem into discovery of two-dimensional symmetry axes on each slice, followed by a robust estimation of plane parameters. The algorithm's tolerance to brain asymmetries, input image offsets and image noise is quantitatively evaluated. We find that the algorithm can extract the iMSP from input 3-D images with 1) large asymmetrical lesions; 2) arbitrary initial rotation offsets; 3) low signal-to-noise ratio or high bias field. The iMSP algorithm is compared with an approach based on maximization of mutual information registration, and is found to exhibit superior performance under adverse conditions. Finally, no statistically significant difference is found between the midsagittal plane computed by the iMSP algorithm and that estimated by two trained neuroradiologists.

  4. Learning-based scan plane identification from fetal head ultrasound images

    NASA Astrophysics Data System (ADS)

    Liu, Xiaoming; Annangi, Pavan; Gupta, Mithun; Yu, Bing; Padfield, Dirk; Banerjee, Jyotirmoy; Krishnan, Kajoli

    2012-03-01

    Acquisition of a clinically acceptable scan plane is a pre-requisite for ultrasonic measurement of anatomical features from B-mode images. In obstetric ultrasound, measurement of gestational age predictors, such as biparietal diameter and head circumference, is performed at the level of the thalami and cavum septum pelucidi. In an accurate scan plane, the head can be modeled as an ellipse, the thalami looks like a butterfly, the cavum appears like an empty box and the falx is a straight line along the major axis of a symmetric ellipse inclined either parallel to or at small angles to the probe surface. Arriving at the correct probe placement on the mother's belly to obtain an accurate scan plane is a task of considerable challenge especially for a new user of ultrasound. In this work, we present a novel automated learning-based algorithm to identify an acceptable fetal head scan plane. We divide the problem into cranium detection and a template matching to capture the composite "butterfly" structure present inside the head, which mimics the visual cues used by an expert. The algorithm uses the stateof- the-art Active Appearance Models techniques from the image processing and computer vision literature and tie them to presence or absence of the inclusions within the head to automatically compute a score to represent the goodness of a scan plane. This automated technique can be potentially used to train and aid new users of ultrasound.

  5. Reproducibility of Frankfort Horizontal Plane on 3D Multi-Planar Reconstructed MR Images

    PubMed Central

    Daboul, Amro; Schwahn, Christian; Schaffner, Grit; Soehnel, Silvia; Samietz, Stefanie; Aljaghsi, Ahmad; Habes, Mohammad; Hegenscheid, Katrin; Puls, Ralf; Klinke, Thomas; Biffar, Reiner

    2012-01-01

    Objective The purpose of this study was to determine the accuracy and reliability of Frankfort horizontal plane identification using displays of multi-planar reconstructed MRI images, and propose it as a sufficiently stable and standardized reference plane for craniofacial structures. Materials and Methods MRI images of 43 subjects were obtained from the longitudinal population based cohort study SHIP-2 using a T1-weighted 3D sequence. Five examiners independently identified the three landmarks that form FH plane. Intra-examiner reproducibility and inter-examiner reliability, correlation coefficients (ICC), coefficient of variability and Bland-Altman plots were obtained for all landmarks coordinates to assess reproducibility. Intra-examiner reproducibility and inter-examiner reliability in terms of location and plane angulation were also assessed. Results Intra- and inter-examiner reliabilities for X, Y and Z coordinates of all three landmarks were excellent with ICC values ranging from 0.914 to 0.998. Differences among examiners were more in X and Z than in Y dimensions. The Bland–Altman analysis demonstrated excellent intra- as well as inter-examiner agreement between examiners in all coordinates for all landmarks. Intra-examiner reproducibility and inter-examiner reliability of the three landmarks in terms of distance showed mean differences between 1.3 to 2.9 mm, Mean differences in plane angulation were between 1.0° to 1.5° among examiners. Conclusion This study revealed excellent intra-examiner reproducibility and inter-examiner reliability of Frankfort Horizontal plane through 3D landmark identification in MRI. Sufficiently stable landmark-based reference plane could be used for different treatments and studies. PMID:23118970

  6. Hard X-ray imaging survey of the Galactic plane with the Caltech gamma-ray imaging payload GRIP-2

    NASA Technical Reports Server (NTRS)

    Corbel, S.; Cook, W. R.; Harrison, F. A.; Prince, T. A.; Schindler, S. M.; Wang, S.

    1997-01-01

    In a two-day balloon flight during October 1995, the Caltech coded aperture gamma ray imaging payload (GRIP-2) imaged various fields in the Galactic plane and center in the 25 to 600 keV energy band. The large phoswich detector, the 15 deg field of view, the 30 arcmin angular resolution and 6 arcmin point source localization capability of GRIP-2 provides the possibility of surveying the accreting binary population of the Galaxy at high energy. The instrument is described and preliminary imaging results are reported on. The capabilities of this instrument for hard X-ray/gamma ray imaging are demonstrated.

  7. Real-time planar segmentation of depth images: from three-dimensional edges to segmented planes

    NASA Astrophysics Data System (ADS)

    Javan Hemmat, Hani; Bondarev, Egor; de With, Peter H. N.

    2015-09-01

    Real-time execution of processing algorithms for handling depth images in a three-dimensional (3-D) data framework is a major challenge. More specifically, considering depth images as point clouds and performing planar segmentation requires heavy computation, because available planar segmentation algorithms are mostly based on surface normals and/or curvatures, and, consequently, do not provide real-time performance. Aiming at the reconstruction of indoor environments, the spaces mainly consist of planar surfaces, so that a possible 3-D application would strongly benefit from a real-time algorithm. We introduce a real-time planar segmentation method for depth images avoiding any surface normal calculation. First, we detect 3-D edges in a depth image and generate line segments between the identified edges. Second, we fuse all the points on each pair of intersecting line segments into a plane candidate. Third and finally, we implement a validation phase to select planes from the candidates. Furthermore, various enhancements are applied to improve the segmentation quality. The GPU implementation of the proposed algorithm segments depth images into planes at the rate of 58 fps. Our pipeline-interleaving technique increases this rate up to 100 fps. With this throughput rate improvement, the application benefit of our algorithm may be further exploited in terms of quality and enhancing the localization.

  8. Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy

    PubMed Central

    Wu, Yicong; Wawrzusin, Peter; Senseney, Justin; Fischer, Robert S; Christensen, Ryan; Santella, Anthony; York, Andrew G; Winter, Peter W; Waterman, Clare M; Bao, Zhirong; Colón-Ramos, Daniel A; McAuliffe, Matthew; Shroff, Hari

    2014-01-01

    Optimal four-dimensional imaging requires high spatial resolution in all dimensions, high speed and minimal photobleaching and damage. We developed a dual-view, plane illumination microscope with improved spatiotemporal resolution by switching illumination and detection between two perpendicular objectives in an alternating duty cycle. Computationally fusing the resulting volumetric views provides an isotropic resolution of 330 nm. As the sample is stationary and only two views are required, we achieve an imaging speed of 200 images/s (i.e., 0.5 s for a 50-plane volume). Unlike spinning-disk confocal or Bessel beam methods, which illuminate the sample outside the focal plane, we maintain high spatiotemporal resolution over hundreds of volumes with negligible photobleaching. To illustrate the ability of our method to study biological systems that require high-speed volumetric visualization and/or low photobleaching, we describe microtubule tracking in live cells, nuclear imaging over 14 h during nematode embryogenesis and imaging of neural wiring during Caenorhabditis elegans brain development over 5 h. PMID:24108093

  9. Magneto-optic imaging: Normal and parallel field components of in-plane magnetized samples

    NASA Astrophysics Data System (ADS)

    Ferrari, H.; Bekeris, V.; Thibeault, M.; Johansen, T. H.

    2007-06-01

    Magneto-optical (MO) imaging has become a powerful tool for determining magnetic properties of materials by detecting the stray magnetic fields. The technique consists in measuring the Faraday rotation, θF, in the light polarization plane when light travels through a transparent sensitive garnet (ferrite garnet film, FGF) placed in close contact to the sample. For in-plane magnetized samples, the MO image is not trivially related to the sample magnetization, and to contribute to this understanding we have imaged commercial audio tapes in which computer-generated functions were recorded. We present MO images of periodically in-plane magnetized tapes with square, sawtooth, triangular and sinusoidal waveforms, for which we analytically calculate the perpendicular and parallel stray magnetic field components generated by the tape. As a first approach we correlate the measured light intensity with the perpendicular magnetic field component at the FGF, and we show that it can be approximated to the gradient of the sample magnetization. A more detailed calculation, taking into account the effect of both field components in the Faraday rotation, is presented and satisfactorily compared with the obtained MO images. The presence of magnetic domains in the garnet is shown to be related to the change in sign of the parallel component of the stray magnetic field, which can be approximated to the second derivative of the sample magnetization.

  10. NIR-green-blue high-resolution digital images for assessement of winter cover crop biomass

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Many small unmanned aerial systems use true-color digital cameras for remote sensing. For some cameras, only the red channel is sensitive to near-infrared (NIR) light; we attached a custom red-blocking filter to a digital camera to obtain NIR-green-blue digital images. One advantage of this low-co...

  11. Electric Field Reconstruction in the Image Plane of a High-Contrast Coronagraph Using a Set of Pinholes around the Lyot Plane

    NASA Technical Reports Server (NTRS)

    Giveona, Amir; Shaklan, Stuart; Kern, Brian; Noecker, Charley; Kendrick, Steve; Wallace, Kent

    2012-01-01

    In a setup similar to the self coherent camera, we have added a set of pinholes in the diffraction ring of the Lyot plane in a high-contrast stellar Lyot coronagraph. We describe a novel complex electric field reconstruction from image plane intensity measurements consisting of light in the coronagraph's dark hole interfering with light from the pinholes. The image plane field is modified by letting light through one pinhole at a time. In addition to estimation of the field at the science camera, this method allows for self-calibration of the probes by letting light through the pinholes in various permutations while blocking the main Lyot opening. We present results of estimation and calibration from the High Contrast Imaging Testbed along with a comparison to the pair-wise deformable mirror diversity based estimation technique. Tests are carried out in narrow-band light and over a composite 10% bandpass.

  12. Ballistic imaging of biological media with collimated illumination and focal plane detection

    NASA Astrophysics Data System (ADS)

    Brezner, Barak; Cahen, Sarah; Glasser, Ziv; Sternklar, Shmuel; Granot, Er'el

    2015-07-01

    A simple, affordable method for imaging through biological tissue is investigated. The method consists of (1) imaging with a wavelength that has a relatively small scattering coefficient (1310 nm in this case) and (2) collimated illumination together with (3) focal plane detection to enhance the detection of the ballistic photons relative to the diffusive light. We demonstrate ballistic detection of an object immersed in a 1-cm-thick cuvette filled with 4% Intralipid, which is equivalent to ˜1 to 2 cm of skin tissue. With the same technology, a ballistic image of a 1-mm-wide object in 10-mm-thick chicken breast is also presented.

  13. Statistical analysis of cross-talk noise and storage capacity in volume holographic memory: image plane holograms

    NASA Astrophysics Data System (ADS)

    Yi, Xianmin; Campbell, Scott; Yeh, Pochi; Gu, Claire

    1995-04-01

    We investigate the cross-talk noise in optical storage based on angle-multiplexed image plane volume holograms. Simple expressions for the signal-to-noise ratio and the storage density are obtained. The cross-talk noise is found to limit the size of the pixels and the number of recorded holograms. The cross-talk-limited storage density of image plane holographic storage is found to be close to that of Fourier plane holographic storage.

  14. Image-based pupil plane characterization via principal component analysis for EUVL tools

    NASA Astrophysics Data System (ADS)

    Levinson, Zac; Burbine, Andrew; Verduijn, Erik; Wood, Obert; Mangat, Pawitter; Goldberg, Kenneth A.; Benk, Markus P.; Wojdyla, Antoine; Smith, Bruce W.

    2016-03-01

    We present an approach to image-based pupil plane amplitude and phase characterization using models built with principal component analysis (PCA). PCA is a statistical technique to identify the directions of highest variation (principal components) in a high-dimensional dataset. A polynomial model is constructed between the principal components of through-focus intensity for the chosen binary mask targets and pupil amplitude or phase variation. This method separates model building and pupil characterization into two distinct steps, thus enabling rapid pupil characterization following data collection. The pupil plane variation of a zone-plate lens from the Semiconductor High-NA Actinic Reticle Review Project (SHARP) at Lawrence Berkeley National Laboratory will be examined using this method. Results will be compared to pupil plane characterization using a previously proposed methodology where inverse solutions are obtained through an iterative process involving least-squares regression.

  15. Multispectral information hiding in RGB image using bit-plane-based watermarking and its application

    NASA Astrophysics Data System (ADS)

    Shinoda, Kazuma; Watanabe, Aya; Hasegawa, Madoka; Kato, Shigeo

    2015-06-01

    Although it was expected that multispectral images would be implemented in many applications, such as remote sensing and medical imaging, their use has not been widely diffused in these fields. The development of a compact multispectral camera and display will be needed for practical use, but the format compatibility between multispectral and RGB images is also important for reducing the introduction cost and having high usability. We propose a method of embedding the spectral information into an RGB image by watermarking. The RGB image is calculated from the multispectral image, and then, the original multispectral image is estimated from the RGB image using Wiener estimation. The residual data between the original and the estimated multispectral image are compressed and embedded in the lower bit planes of the RGB image. The experimental results show that, as compared with Wiener estimation, the proposed method leads to more than a 10 dB gain in the peak signal-to-noise ratio of the reconstructed multispectral image, while there are almost no significant perceptual differences in the watermarked RGB image.

  16. Multimodal Randomized Functional MR Imaging of the Effects of Methylene Blue in the Human Brain.

    PubMed

    Rodriguez, Pavel; Zhou, Wei; Barrett, Douglas W; Altmeyer, Wilson; Gutierrez, Juan E; Li, Jinqi; Lancaster, Jack L; Gonzalez-Lima, Francisco; Duong, Timothy Q

    2016-11-01

    Purpose To investigate the sustained-attention and memory-enhancing neural correlates of the oral administration of methylene blue in the healthy human brain. Materials and Methods The institutional review board approved this prospective, HIPAA-compliant, randomized, double-blinded, placebo-controlled clinical trial, and all patients provided informed consent. Twenty-six subjects (age range, 22-62 years) were enrolled. Functional magnetic resonance (MR) imaging was performed with a psychomotor vigilance task (sustained attention) and delayed match-to-sample tasks (short-term memory) before and 1 hour after administration of low-dose methylene blue or a placebo. Cerebrovascular reactivity effects were also measured with the carbon dioxide challenge, in which a 2 × 2 repeated-measures analysis of variance was performed with a drug (methylene blue vs placebo) and time (before vs after administration of the drug) as factors to assess drug × time between group interactions. Multiple comparison correction was applied, with cluster-corrected P < .05 indicating a significant difference. Results Administration of methylene blue increased response in the bilateral insular cortex during a psychomotor vigilance task (Z = 2.9-3.4, P = .01-.008) and functional MR imaging response during a short-term memory task involving the prefrontal, parietal, and occipital cortex (Z = 2.9-4.2, P = .03-.0003). Methylene blue was also associated with a 7% increase in correct responses during memory retrieval (P = .01). Conclusion Low-dose methylene blue can increase functional MR imaging activity during sustained attention and short-term memory tasks and enhance memory retrieval. (©) RSNA, 2016 Online supplemental material is available for this article.

  17. Range-gated imaging with an indium-gallium-arsenide-based focal plane array

    NASA Astrophysics Data System (ADS)

    Brubaker, Robert M.; Ettenberg, Martin H.; O'Grady, Matthew T.; Blessinger, Michael A.; Dries, J. C.

    2004-08-01

    Range-gated imaging using indium gallium arsenide based focal plane arrays enables both depth and intensity imaging with eye-safe lasers while remaining covert to night vision goggles. We report on a focal plane array consisting of an indium gallium arsenide photodiode array hybrid-integrated with a CMOS readout circuit, resulting in an all solid state device. A 5 V supply avoids the complication of high voltage supplies and improves reliability, while also allowing the device to be small and lightweight. The spectral sensitivity of InGaAs extends from 0.9 microns to 1.7 microns, allowing the use of commercially available pulsed lasers with 1.5 micron wavelength, several millijoule pulse energies, and nanosecond scale pulse durations. SUI is developing a 320 x 256 pixel imager with the ability to conduct range gated imaging with sub-100 ns gates, while also allowing a 16 ms integration time for imaging in a staring mode. The pixels are fabricated on a 25 micron pitch for a compact device, and all pixels are gated simultaneously for "snapshot" exposure. High in-pixel gain with nearly noiseless amplification and low dark current enable high sensitivity imaging from ultra-short gates to video rate imaging.

  18. High resolution image reconstruction method for a double-plane PET system with changeable spacing

    NASA Astrophysics Data System (ADS)

    Gu, Xiao-Yue; Zhou, Wei; Li, Lin; Wei, Long; Yin, Peng-Fei; Shang, Lei-Min; Yun, Ming-Kai; Lu, Zhen-Rui; Huang, Xian-Chao

    2016-05-01

    Breast-dedicated positron emission tomography (PET) imaging techniques have been developed in recent years. Their capacities to detect millimeter-sized breast tumors have been the subject of many studies. Some of them have been confirmed with good results in clinical applications. With regard to biopsy application, a double-plane detector arrangement is practicable, as it offers the convenience of breast immobilization. However, the serious blurring effect of the double-plane PET, with changeable spacing for different breast sizes, should be studied. We investigated a high resolution reconstruction method applicable for a double-plane PET. The distance between the detector planes is changeable. Geometric and blurring components were calculated in real-time for different detector distances, and accurate geometric sensitivity was obtained with a new tube area model. Resolution recovery was achieved by estimating blurring effects derived from simulated single gamma response information. The results showed that the new geometric modeling gave a more finite and smooth sensitivity weight in the double-plane PET. The blurring component yielded contrast recovery levels that could not be reached without blurring modeling, and improved visual recovery of the smallest spheres and better delineation of the structures in the reconstructed images were achieved with the blurring component. Statistical noise had lower variance at the voxel level with blurring modeling at matched resolution, compared to without blurring modeling. In distance-changeable double-plane PET, finite resolution modeling during reconstruction achieved resolution recovery, without noise amplification. Supported by Knowledge Innovation Project of The Chinese Academy of Sciences (KJCX2-EW-N06)

  19. Lyot-plane phase masks for improved high-contrast imaging with a vortex coronagraph

    NASA Astrophysics Data System (ADS)

    Ruane, G. J.; Huby, E.; Absil, O.; Mawet, D.; Delacroix, C.; Carlomagno, B.; Swartzlander, G. A.

    2015-11-01

    Context. The vortex coronagraph is an optical instrument that precisely removes on-axis starlight allowing for high contrast imaging at small angular separation from the star, a crucial capability for direct detection and characterization of exoplanets and circumstellar disks. Telescopes with aperture obstructions, such as secondary mirrors and spider support structures, require advanced coronagraph designs to provide adequate starlight suppression. Aims: We introduce a phase-only Lyot-plane optic to the vortex coronagraph, which offers improved contrast performance on telescopes with complicated apertures. Potential solutions for the European Extremely Large Telescope (E-ELT) are described. Methods: Adding a Lyot-plane phase mask relocates residual starlight away from a region of the image plane, thereby reducing stellar noise and improving sensitivity to off-axis companions. The phase mask is calculated using an iterative phase retrieval algorithm. Results: Numerically, we achieve a contrast on the order of 10-6 for a companion with angular displacement as small as 4λ/D with an E-ELT type aperture. Even in the presence of aberrations, improved performance is expected compared to either a conventional vortex coronagraph or an optimized pupil plane phase element alone.

  20. Conceptual design of wide-field focal plane with InGaAs image sensors

    NASA Astrophysics Data System (ADS)

    Komiyama, Y.; Nakaya, H.; Kashikawa, N.; Uchida, T.

    2016-08-01

    We present a conceptual design to implement wide-field focal plane assembly with InGaAs image sensors which are being tested intensively and reveled to be promising for astronomical use. InGaAs image sensors are sensitive up to 1.7 microns and would open a new window for the wide-field near-infrared (NIR) imaging survey once large format sensors are developed. The sensors are not necessarily cooled down to below 100 K, which is the case for prevailing NIR image sensors such as HgCdTe, enabling us to develop the NIR camera based on the technique developed for the CCD camera in optical wavelength. The major technical challenges to employ InGaAS image sensors for wide-field NIR camera are implementation of focal plane assembly and thermal design. In this article, we discuss these difficulties and show how we can conquer based on our experience to build Hyper Suprime-Cam, which is a wide-field imager with 116 2k4k CCDs attached to Subaru Telescope.

  1. Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging.

    PubMed

    Engelbrecht, Christoph J; Voigt, Fabian; Helmchen, Fritjof

    2010-05-01

    Light-sheet-based fluorescence imaging techniques rely on simultaneous excitation of a single optical plane and thus permit high-contrast optically sectioned imaging of extended tissue samples. Here, we introduce a miniaturized fiber-optic implementation of a selective plane-illumination microscope (miniSPIM). The excitation light was delivered through a single-mode optical fiber, and a light-sheet was created with a cylindrical gradient-index lens and a right-angle microprism. Fluorescence emission was collected orthogonally to the light-sheet through a gradient-index lens assembly and a coherent fiber bundle. The end face of the fiber bundle was imaged onto a charge-coupled device camera. The spatial resolutions of the miniSPIM were 3.2 microm laterally and 5.1 microm axially. Images of fluorescent beads and neurons in mouse neocortex exhibited superior axial resolution and contrast in the miniSPIM-mode compared to images recorded in epi-illumination mode. The miniSPIM may thus enable novel in vivo imaging approaches.

  2. Mid-level image representations for real-time heart view plane classification of echocardiograms.

    PubMed

    Penatti, Otávio A B; Werneck, Rafael de O; de Almeida, Waldir R; Stein, Bernardo V; Pazinato, Daniel V; Mendes Júnior, Pedro R; Torres, Ricardo da S; Rocha, Anderson

    2015-11-01

    In this paper, we explore mid-level image representations for real-time heart view plane classification of 2D echocardiogram ultrasound images. The proposed representations rely on bags of visual words, successfully used by the computer vision community in visual recognition problems. An important element of the proposed representations is the image sampling with large regions, drastically reducing the execution time of the image characterization procedure. Throughout an extensive set of experiments, we evaluate the proposed approach against different image descriptors for classifying four heart view planes. The results show that our approach is effective and efficient for the target problem, making it suitable for use in real-time setups. The proposed representations are also robust to different image transformations, e.g., downsampling, noise filtering, and different machine learning classifiers, keeping classification accuracy above 90%. Feature extraction can be performed in 30 fps or 60 fps in some cases. This paper also includes an in-depth review of the literature in the area of automatic echocardiogram view classification giving the reader a through comprehension of this field of study.

  3. A method for pulsed scannerless laser imaging using focal plane array

    NASA Astrophysics Data System (ADS)

    Zhang, Zhi-peng; Wang, Ke-yong; Deng, Jia-hao; Hai, Yan

    2011-06-01

    Laser imaging techniques have advantages for EMI (Electro Magnetic Interference) immunity and abundant image information. This contribution describes the research activity on the scannerless laser imaging detection technique using direct detection aimed at laser fuze applications. The technique using a pulsed laser to illuminate the target and a focal plane array can be used as a receiver. The range information is achieved by means of a direct time of light measurement. Information about the reflectivity of the target is gathered by recording the amplitude of the received pulse. In this paper a high-repetition-frequency, narrow pulse semiconductor laser floodlight emitting system is designed; corresponding optics is used to generate the homogenously illuminated FOI (field of illumination). The echo of laser is collected by receiving optical system fed to focal plane array. Some experiments were done with the emitting and receiving systems that had been designed. Experiments show the validity and rationality of this method. The scannerless structure is robust and provides instantaneous snapshot-type imaging. Avoiding any moving mechanical parts, scannerless laser imaging system have distinct characteristics such as small, compact, high frame rate, wide field of view and high reliability. It is an optimal approach to realize laser imaging fuze.

  4. Uncooled bolometer-type Terahertz focal plane array and camera for real-time imaging

    NASA Astrophysics Data System (ADS)

    Oda, Naoki

    2010-08-01

    Real-time Terahertz (THz) imaging technologies which make use of uncooled bolometer-type infrared focal plane arrays (FPAs) and quantum cascade lasers (QCLs) will be reviewed. A description of how THz focal plane array and THz imagers have been developed on the basis of infrared technologies, especially the improvement in both THz sensitivity of bolometer-type FPA and THz transmittance of materials for lens and vacuum package window will be given. Characteristics of 320×240 THz-FPA, such as relation of noise equivalent power (NEP) to wavelength and real-time THz imageries will be presented. One of the imageries indicates that THz technology is promising for label-free detection of reaction of small molecules with proteins.

  5. Near Real-Time Imaging of the Galactic Plane with BATSE

    NASA Technical Reports Server (NTRS)

    Harmon, B. A.; Zhang, S. N.; Robinson, C. R.; Paciesas, W. S.; Barret, D.; Grindlay, J.; Bloser, P.; Monnelly, C.

    1997-01-01

    The discovery of new transient or persistent sources in the hard X-ray regime with the BATSE Earth occultation Technique has been limited previously to bright sources of about 200 mCrab or more. While monitoring known source locations is not a problem to a daily limiting sensitivity of about 75 mCrab, the lack of a reliable background model forces us to use more intensive computer techniques to find weak, previously unknown emission from hard X-ray/gamma sources. The combination of Radon transform imaging of the galactic plane in 10 by 10 degree fields and the Harvard/CFA-developed Image Search (CBIS) allows us to straightforwardly search the sky for candidate sources in a +/- 20 degree latitude band along the plane. This procedure has been operating routinely on a weekly basis since spring 1997. We briefly describe the procedure, then concentrate on the performance aspects of the technique and candidate source results from the search.

  6. Effects of Non-Elevation-Focalized Linear Array Transducer on Ultrasound Plane-Wave Imaging

    PubMed Central

    Wang, Congzhi; Xiao, Yang; Xia, Jingjing; Qiu, Weibao; Zheng, Hairong

    2016-01-01

    Plane-wave ultrasound imaging (PWUS) has become an important method of ultrasound imaging in recent years as its frame rate has exceeded 10,000 frames per second, allowing ultrasound to be used for two-dimensional shear wave detection and functional brain imaging. However, compared to the traditional focusing and scanning method, PWUS images always suffer from a degradation of lateral resolution and contrast. To improve the image quality of PWUS, many different beamforming algorithms have been proposed and verified. Yet the influence of transducer structure is rarely studied. For this paper, the influence of using an acoustic lens for PWUS was evaluated. Two linear array transducers were fabricated. One was not self-focalized in the elevation direction (non-elevation-focalized transducer, NEFT); the other one was a traditional elevation-focalized transducer (EFT). An initial simulation was conducted to show the influence of elevation focusing. Then the images obtained with NEFT on a standard ultrasound imaging phantom were compared with those obtained with EFT. It was demonstrated that, in a relatively deep region, the contrast of an NEFT image is better than that of an EFT image. These results indicate that a more sophisticated design of ultrasound transducer would further improve the image quality of PWUS. PMID:27845751

  7. Fast functional imaging of multiple brain regions in intact zebrafish larvae using selective plane illumination microscopy.

    PubMed

    Panier, Thomas; Romano, Sebastián A; Olive, Raphaël; Pietri, Thomas; Sumbre, Germán; Candelier, Raphaël; Debrégeas, Georges

    2013-01-01

    The optical transparency and the small dimensions of zebrafish at the larval stage make it a vertebrate model of choice for brain-wide in-vivo functional imaging. However, current point-scanning imaging techniques, such as two-photon or confocal microscopy, impose a strong limit on acquisition speed which in turn sets the number of neurons that can be simultaneously recorded. At 5 Hz, this number is of the order of one thousand, i.e., approximately 1-2% of the brain. Here we demonstrate that this limitation can be greatly overcome by using Selective-plane Illumination Microscopy (SPIM). Zebrafish larvae expressing the genetically encoded calcium indicator GCaMP3 were illuminated with a scanned laser sheet and imaged with a camera whose optical axis was oriented orthogonally to the illumination plane. This optical sectioning approach was shown to permit functional imaging of a very large fraction of the brain volume of 5-9-day-old larvae with single- or near single-cell resolution. The spontaneous activity of up to 5,000 neurons was recorded at 20 Hz for 20-60 min. By rapidly scanning the specimen in the axial direction, the activity of 25,000 individual neurons from 5 different z-planes (approximately 30% of the entire brain) could be simultaneously monitored at 4 Hz. Compared to point-scanning techniques, this imaging strategy thus yields a ≃20-fold increase in data throughput (number of recorded neurons times acquisition rate) without compromising the signal-to-noise ratio (SNR). The extended field of view offered by the SPIM method allowed us to directly identify large scale ensembles of neurons, spanning several brain regions, that displayed correlated activity and were thus likely to participate in common neural processes. The benefits and limitations of SPIM for functional imaging in zebrafish as well as future developments are briefly discussed.

  8. Comparison of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

    NASA Astrophysics Data System (ADS)

    Wu, Chen; Ran, Shihao; Le, Henry H.; Singh, Manmohan; Larina, Irina V.; Mayerich, David; Dickinson, Mary E.; Larin, Kirill V.

    2016-03-01

    The mouse is a common model for studying developmental diseases. Different optical techniques have been developed to investigate mouse embryos, but each has its own set of limitations and restrictions. In this study, we imaged the same E9.5 mouse embryo with rotational imaging Optical Coherence Tomography (RI-OCT) and Selective Plane Illumination Microscopy (SPIM), and compared the two techniques. Results demonstrate that both methods can provide images with micrometer-scale spatial resolution. The RI-OCT technique was developed to increase imaging depth of OCT by performing traditional OCT imaging at multiple sides and co-registering the images. In SPIM, optical sectioning is achieved by illuminating the sample with a sheet of light. In this study, the images acquired from both techniques are compared with each other to evaluate the benefits and drawbacks of each technique for embryonic imaging. Since 3D stacks can be obtained by SPIM from different angles by rotating the sample, it might be possible to build a hybrid setup of two imaging modalities to combine the advantages of each technique.

  9. Multispectral Thermal Imager Optical Assembly Performance and Intergration of the Flight Focal Plane Assembly

    SciTech Connect

    Blake, Dick; Byrd, Don; Christensen, Wynn; Henson, Tammy; Krumel, Les; Rappoport, William; Shen, Gon-Yen

    1999-06-08

    The Multispectral Thermal Imager Optical Assembly (OA) has been fabricated, assembled, successfully performance tested, and integrated into the flight payload structure with the flight Focal Plane Assembly (FPA) integrated and aligned to it. This represents a major milestone achieved towards completion of this earth observing E-O imaging sensor that is to be operated in low earth orbit. The OA consists of an off-axis three mirror anastigmatic (TMA) telescope with a 36 cm unobscured clear aperture, a wide-field-of-view (WFOV) of 1.82° along the direction of spacecraft motion and 1.38° across the direction of spacecraft motion. It also contains a comprehensive on-board radiometric calibration system. The OA is part of a multispectral pushbroom imaging sensor which employs a single mechanically cooled focal plane with 15 spectral bands covering a wavelength range from 0.45 to 10.7 µm. The OA achieves near diffraction-limited performance from visible to the long-wave infrared (LWIR) wavelengths. The two major design drivers for the OA are 80% enpixeled energy in the visible bands and radiometric stability. Enpixeled energy in the visible bands also drove the alignment of the FPA detectors to the OA image plane to a requirement of less than ± 20 µm over the entire visible detector field of view (FOV). Radiometric stability requirements mandated a cold Lyot stop for stray light rejection and thermal background reduction. The Lyot stop is part of the FPA assembly and acts as the aperture stop for the imaging system. The alignment of the Lyot stop to the OA drove the centering and to some extent the tilt alignment requirements of the FPA to the OA.

  10. A blue optical filter for narrow-band imaging in endoscopic capsules

    NASA Astrophysics Data System (ADS)

    Silva, M. F.; Ghaderi, M.; Goncalves, L. M.; de Graaf, G.; Wolffenbuttel, R. F.; Correia, J. H.

    2014-05-01

    This paper presents the design, simulation, fabrication, and characterization of a thin-film Fabry-Perot resonator composed of titanium dioxide (TiO2) and silicon dioxide (SiO2) thin-films. The optical filter is developed to be integrated with a light emitting diode (LED) for enabling narrow-band imaging (NBI) in endoscopy. The NBI is a high resolution imaging technique that uses spectrally centered blue light (415 nm) and green light (540 nm) to illuminate the target tissue. The light at 415 nm enhances the imaging of superficial veins due to their hemoglobin absorption, while the light at 540 nm penetrates deeper into the mucosa, thus enhances the sub-epithelial vessels imaging. Typically the endoscopes and endoscopic capsules use white light for acquiring images of the gastrointestinal (GI) tract. However, implementing the NBI technique in endoscopic capsules enhances their capabilities for the clinical applications. A commercially available blue LED with a maximum peak intensity at 404 nm and Full Width Half Maximum (FWHM) of 20 nm is integrated with a narrow band blue filter as the NBI light source. The thin film simulations show a maximum spectral transmittance of 36 %, that is centered at 415 nm with FWHM of 13 nm for combined the blue LED and a Fabry Perot resonator system. A custom made deposition scheme was developed for the fabrication of the blue optical filter by RF sputtering. RF powered reactive sputtering at 200 W with the gas flows of argon and oxygen that are controlled for a 5:1 ratio gives the optimum optical conditions for TiO2 thin films. For SiO2 thin films, a non-reactive RF sputtering at 150 W with argon gas flow at 15 sccm results in the best optical performance. The TiO2 and SiO2 thin films were fully characterized by an ellipsometer in the wavelength range between 250 nm to 1600 nm. Finally, the optical performance of the blue optical filter is measured and presented.

  11. Midsagittal plane extraction from brain images based on 3D SIFT

    NASA Astrophysics Data System (ADS)

    Wu, Huisi; Wang, Defeng; Shi, Lin; Wen, Zhenkun; Ming, Zhong

    2014-03-01

    Midsagittal plane (MSP) extraction from 3D brain images is considered as a promising technique for human brain symmetry analysis. In this paper, we present a fast and robust MSP extraction method based on 3D scale-invariant feature transform (SIFT). Unlike the existing brain MSP extraction methods, which mainly rely on the gray similarity, 3D edge registration or parameterized surface matching to determine the fissure plane, our proposed method is based on distinctive 3D SIFT features, in which the fissure plane is determined by parallel 3D SIFT matching and iterative least-median of squares plane regression. By considering the relative scales, orientations and flipped descriptors between two 3D SIFT features, we propose a novel metric to measure the symmetry magnitude for 3D SIFT features. By clustering and indexing the extracted SIFT features using a k-dimensional tree (KD-tree) implemented on graphics processing units, we can match multiple pairs of 3D SIFT features in parallel and solve the optimal MSP on-the-fly. The proposed method is evaluated by synthetic and in vivo datasets, of normal and pathological cases, and validated by comparisons with the state-of-the-art methods. Experimental results demonstrated that our method has achieved a real-time performance with better accuracy yielding an average yaw angle error below 0.91° and an average roll angle error no more than 0.89°.

  12. Optical Measurement of In-plane Waves in Mechanical Metamaterials Through Digital Image Correlation

    NASA Astrophysics Data System (ADS)

    Schaeffer, Marshall; Trainiti, Giuseppe; Ruzzene, Massimo

    2017-02-01

    We report on a Digital Image Correlation-based technique for the detection of in-plane elastic waves propagating in structural lattices. The experimental characterization of wave motion in lattice structures is currently of great interest due its relevance to the design of novel mechanical metamaterials with unique/unusual properties such as strongly directional behaviour, negative refractive indexes and topologically protected wave motion. Assessment of these functionalities often requires the detection of highly spatially resolved in-plane wavefields, which for reticulated or porous structural assemblies is an open challenge. A Digital Image Correlation approach is implemented that tracks small displacements of the lattice nodes by centring image subsets about the lattice intersections. A high speed camera records the motion of the points by properly interleaving subse- quent frames thus artificially enhancing the available sampling rate. This, along with an imaging stitching procedure, enables the capturing of a field of view that is sufficiently large for subsequent processing. The transient response is recorded in the form of the full wavefields, which are processed to unveil features of wave motion in a hexagonal lattice. Time snapshots and frequency contours in the spatial Fourier domain are compared with numerical predictions to illustrate the accuracy of the recorded wavefields.

  13. InstantScope: a low-cost whole slide imaging system with instant focal plane detection.

    PubMed

    Guo, Kaikai; Liao, Jun; Bian, Zichao; Heng, Xin; Zheng, Guoan

    2015-09-01

    We report the development of a high-throughput whole slide imaging (WSI) system by adapting a cost-effective optomechanical add-on kit to existing microscopes. Inspired by the phase detection concept in professional photography, we attached two pinhole-modulated cameras at the eyepiece ports for instant focal plane detection. By adjusting the positions of the pinholes, we can effectively change the view angle for the sample, and as such, we can use the translation shift of the two pinhole-modulated images to identify the optimal focal position. By using a small pinhole size, the focal-plane-detection range is on the order of millimeter, orders of magnitude longer than the objective's depth of field. We also show that, by analyzing the phase correlation of the pinhole-modulated images, we can determine whether the sample contains one thin section, folded sections, or multiple layers separated by certain distances - an important piece of information prior to a detailed z scan. In order to achieve system automation, we deployed a low-cost programmable robotic arm to perform sample loading and $14 stepper motors to drive the microscope stage to perform x-y scanning. Using a 20X objective lens, we can acquire a 2 gigapixel image with 14 mm by 8 mm field of view in 90 seconds. The reported platform may find applications in biomedical research, telemedicine, and digital pathology. It may also provide new insights for the development of high-content screening instruments.

  14. Optical Measurement of In-plane Waves in Mechanical Metamaterials Through Digital Image Correlation

    PubMed Central

    Schaeffer, Marshall; Trainiti, Giuseppe; Ruzzene, Massimo

    2017-01-01

    We report on a Digital Image Correlation-based technique for the detection of in-plane elastic waves propagating in structural lattices. The experimental characterization of wave motion in lattice structures is currently of great interest due its relevance to the design of novel mechanical metamaterials with unique/unusual properties such as strongly directional behaviour, negative refractive indexes and topologically protected wave motion. Assessment of these functionalities often requires the detection of highly spatially resolved in-plane wavefields, which for reticulated or porous structural assemblies is an open challenge. A Digital Image Correlation approach is implemented that tracks small displacements of the lattice nodes by centring image subsets about the lattice intersections. A high speed camera records the motion of the points by properly interleaving subse- quent frames thus artificially enhancing the available sampling rate. This, along with an imaging stitching procedure, enables the capturing of a field of view that is sufficiently large for subsequent processing. The transient response is recorded in the form of the full wavefields, which are processed to unveil features of wave motion in a hexagonal lattice. Time snapshots and frequency contours in the spatial Fourier domain are compared with numerical predictions to illustrate the accuracy of the recorded wavefields. PMID:28205589

  15. Optical Measurement of In-plane Waves in Mechanical Metamaterials Through Digital Image Correlation.

    PubMed

    Schaeffer, Marshall; Trainiti, Giuseppe; Ruzzene, Massimo

    2017-02-13

    We report on a Digital Image Correlation-based technique for the detection of in-plane elastic waves propagating in structural lattices. The experimental characterization of wave motion in lattice structures is currently of great interest due its relevance to the design of novel mechanical metamaterials with unique/unusual properties such as strongly directional behaviour, negative refractive indexes and topologically protected wave motion. Assessment of these functionalities often requires the detection of highly spatially resolved in-plane wavefields, which for reticulated or porous structural assemblies is an open challenge. A Digital Image Correlation approach is implemented that tracks small displacements of the lattice nodes by centring image subsets about the lattice intersections. A high speed camera records the motion of the points by properly interleaving subse- quent frames thus artificially enhancing the available sampling rate. This, along with an imaging stitching procedure, enables the capturing of a field of view that is sufficiently large for subsequent processing. The transient response is recorded in the form of the full wavefields, which are processed to unveil features of wave motion in a hexagonal lattice. Time snapshots and frequency contours in the spatial Fourier domain are compared with numerical predictions to illustrate the accuracy of the recorded wavefields.

  16. Medical image compression using cubic spline interpolation with bit-plane compensation

    NASA Astrophysics Data System (ADS)

    Truong, Trieu-Kien; Chen, Shi-Huang; Lin, Tsung-Ching

    2007-03-01

    In this paper, a modified medical image compression algorithm using cubic spline interpolation (CSI) is presented for telemedicine applications. The CSI is developed in order to subsample image data with minimal distortion and to achieve compression. It has been shown in literatures that the CSI can be combined with the JPEG algorithms to develop a modified JPEG codec, which obtains a higher compression ratio and a better quality of reconstructed image than the standard JPEG. However, this modified JPEG codec will lose some high-frequency components of medical images during compression process. To minimize the drawback arose from loss of these high-frequency components, this paper further makes use of bit-plane compensation to the modified JPEG codec. The bit-plane compensation algorithm used in this paper is modified from JBIG2 standard. Experimental results show that the proposed scheme can increase 20~30% compression ratio of original JPEG medical data compression system with similar visual quality. This system can reduce the loading of telecommunication networks and is quite suitable for low bit-rate telemedicine applications.

  17. Accuracy of image-plane holographic tomography with filtered backprojection: random and systematic errors.

    PubMed

    Belashov, A V; Petrov, N V; Semenova, I V

    2016-01-01

    This paper explores the concept of image-plane holographic tomography applied to the measurements of laser-induced thermal gradients in an aqueous solution of a photosensitizer with respect to the reconstruction accuracy of three-dimensional variations of the refractive index. It uses the least-squares estimation algorithm to reconstruct refractive index variations in each holographic projection. Along with the bitelecentric optical system, transferring focused projection to the sensor plane, it facilitates the elimination of diffraction artifacts and noise suppression. This work estimates the influence of typical random and systematic errors in experiments and concludes that random errors such as accidental measurement errors or noise presence can be significantly suppressed by increasing the number of recorded digital holograms. On the contrary, even comparatively small systematic errors such as a displacement of the rotation axis projection in the course of a reconstruction procedure can significantly distort the results.

  18. The Second Epoch Molonglo Galactic Plane Survey: Images and Candidate Supernova Remnants

    NASA Astrophysics Data System (ADS)

    Green, A. J.; Reeves, S. N.; Murphy, T.

    2014-11-01

    The second epoch Molonglo Galactic Plane Survey covers the area 245° ⩽ l ⩽ 365° and |b| ⩽ 10° at a frequency of 843 MHz and an angular resolution of 45 arcsec × 45 arcsec cosec(δ). The sensitivity varies between 1-2 mJy beam- 1 depending on the presence of strong extended sources. This survey is currently the highest resolution and most sensitive large-scale continuum survey of the southern Galactic plane. In this paper, we present the images of the complete survey, including postage stamps of some new supernova remnant (SNR) candidates and a discussion of the highly structured features detected in the interstellar medium. The intersection of these two types of features is discussed in the context of the `missing' SNR population in the Galaxy.

  19. Multiple Plane Phase Retrieval Based On Inverse Regularized Imaging and Discrete Diffraction Transform

    NASA Astrophysics Data System (ADS)

    Migukin, Artem; Katkovnik, Vladimir; Astola, Jaakko

    2010-04-01

    The phase retrieval is formulated as an inverse problem, where the forward propagation is defined by Discrete Diffraction Transform (DDT) [1], [2]. This propagation model is precise and aliasing free for pixelwise invariant (pixelated) wave field distributions in the sensor and object planes. Because of finite size of sensors DDT can be ill-conditioned and the regularization is an important component of the inverse. The proposed algorithm is designed for multiple plane observations and can be treated as a generalization of the Gerchberg-Saxton iterative algorithm. The proposed algorithm is studied by numerical experiments produced for phase and amplitude modulated object distributions. Comparison versus the conventional forward propagation models such as the angular spectrum decomposition and the convolutional model used in the algorithm of the same structure shows a clear advantage of DDT enabling better accuracy and better imaging.

  20. Assessment of methods to extract the mid-sagittal plane from brain MR images

    NASA Astrophysics Data System (ADS)

    Kuijf, Hugo J.; Leemans, Alexander; Viergever, Max A.; Vincken, Koen L.

    2013-03-01

    Automatic detection of the mid-sagittal plane, separating both hemispheres of the brain, is useful in various applications. Several methods have been developed in the past years, applying different techniques to estimate the position of the mid-sagittal plane. These methods can be classified into three distinct classes: feature-based, global symmetry based, and local symmetry based methods. Feature-based methods use the shape or intensity of the interhemispheric fissure to extract the mid-sagittal plane. Global symmetry based methods reflect the entire image with respect to the sagittal axes and perform a rigid registration. Local symmetry based methods try to optimize a symmetry-measure in a small band covering the interhemispheric fissure. From each class, one leading method has been implemented. The methods have been evaluated on the same datasets to allow a fair comparison. Manual delineations were made by two experienced human observers. The results show that the examined methods perform similar to human observers. No significant differences were found between errors (defined as the angle and volume between planes) made by the methods and the inter-observer differences. Feature-based and local symmetry based methods have a low computation time of 1.8 and 0.5 seconds, respectively. The global symmetry based method has a higher computation time of 33.6 seconds, caused by the full 3D rigid registration. The largest errors, both by the methods and observers, are made in participants with cerebral atrophy. These participants have a widened interhemispheric fissure, allowing many plane orientations and positions to result in a valid division of the hemispheres.

  1. A Design of Focal-plane Compensation of Aviation Imaging Equipment Based on MS5534C

    NASA Astrophysics Data System (ADS)

    Lina, Zheng; Xue, Leng; Jiufei, Zhou; Yong, He; Jinbao, Fu

    This paper proposes an auto-compensation method for defocusing distance caused by temperature and pressure in aviation imaging equipment. As the host computer, the TMS320F2812 is the core controller and the digital pressure sensor MS5534C from Intersema Company is used as slave computer. The controller acquires the output of the temperature and the pressure from the sensor through MCBSP interface. By the change of temperature and pressure which results in defocusing distance, the software is adopted to compensate the defocusing distance and thereby keeps the stabilization of focal plane in aviation imaging equipment. The design proposal and the software flow is shown in the paper, furthermore the new system has simple interface, small size and real-time function. With many flight tests, the defocusing distance after the compensation of temperature and pressure is far less than the half focal depth of the optical system and it is fully satisfied with the requirements of imaging.

  2. Selective-plane illumination microscopy for high-content volumetric biological imaging

    NASA Astrophysics Data System (ADS)

    McGorty, Ryan; Huang, Bo

    2016-03-01

    Light-sheet microscopy, also named selective-plane illumination microscopy, enables optical sectioning with minimal light delivered to the sample. Therefore, it allows one to gather volumetric datasets of developing embryos and other light-sensitive samples over extended times. We have configured a light-sheet microscope that, unlike most previous designs, can image samples in formats compatible with high-content imaging. Our microscope can be used with multi-well plates or with microfluidic devices. In designing our optical system to accommodate these types of sample holders we encounter large optical aberrations. We counter these aberrations with both static optical components in the imaging path and with adaptive optics. Potential applications of this microscope include studying the development of a large number of embryos in parallel and over long times with subcellular resolution and doing high-throughput screens on organisms or cells where volumetric data is necessary.

  3. Three-dimensional fluorescence imaging by stage-scanning oblique plane microscopy (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Maioli, Vincent; Görlitz, Frederik; Warren, Sean; Kumar, Sunil; French, Paul M. W.; Chennell, George; Sardini, Alessandro; Carling, David; Alwes, Frederike; Dunsby, Christopher W.

    2016-03-01

    Oblique plane microscopy (OPM) is a light-sheet fluorescence microscopy technique that is implemented on a standard inverted microscope frame. OPM uses a single high numerical aperture microscope objective to both produce a tilted excitation light-sheet and to image the fluorescence emitted from the tilted plane back to the cameras. It is therefore compatible with conventional sample-mounting techniques such as microscope slides and multiwell plates. Four excitation laser lines and two high-speed sCMOS cameras with separate emission filters enable the simultaneous imaging of several fluorophores and spectral ratiometric FRET acquisitions. Previously, 3-D OPM imaging has been implemented by remote refocusing. Here, a stage-scanning approach to 3-D OPM imaging is demonstrated - enabling three-dimensional multi-channel acquisition including of multiwell plates - and the synchronization of the stage movement and camera acquisition will be described. The ability of the stage-scanning system to image fields of view larger than the field of view of the primary microscope objective is demonstrated using fluorescently labelled limbs of crustaceans and its ability to perform time-lapse 3-D imaging over 12 hours is demonstrated using a sample of tumor spheroids with an acquisition time of 3 s for a typical spheroid providing 400x1280x1024 voxels per spheroid. We also apply the system to spectral ratiometric Förster resonant energy transfer (FRET) measurements in tumor spheroids expressing a FRET biosensor and in a 96-well plate seeded with cell samples expressing varying concentrations of a FRETting and non-FRETting constructs.

  4. Evaluation of an image-based tracking workflow with Kalman filtering for automatic image plane alignment in interventional MRI.

    PubMed

    Neumann, M; Cuvillon, L; Breton, E; de Matheli, M

    2013-01-01

    Recently, a workflow for magnetic resonance (MR) image plane alignment based on tracking in real-time MR images was introduced. The workflow is based on a tracking device composed of 2 resonant micro-coils and a passive marker, and allows for tracking of the passive marker in clinical real-time images and automatic (re-)initialization using the microcoils. As the Kalman filter has proven its benefit as an estimator and predictor, it is well suited for use in tracking applications. In this paper, a Kalman filter is integrated in the previously developed workflow in order to predict position and orientation of the tracking device. Measurement noise covariances of the Kalman filter are dynamically changed in order to take into account that, according to the image plane orientation, only a subset of the 3D pose components is available. The improved tracking performance of the Kalman extended workflow could be quantified in simulation results. Also, a first experiment in the MRI scanner was performed but without quantitative results yet.

  5. Underwater image quality enhancement through Rayleigh-stretching and averaging image planes

    NASA Astrophysics Data System (ADS)

    Ghani, Ahmad Shahrizan Abdul; Isa, Nor Ashidi Mat

    2014-12-01

    Visibility in underwater images is usually poor because of the attenuation of light in the water that causes low contrast and color variation. In this paper, a new approach for underwater image quality improvement is presented. The proposed method aims to improve underwater image contrast, increase image details, and reduce noise by applying a new method of using contrast stretching to produce two different images with different contrasts. The proposed method integrates the modification of the image histogram in two main color models, RGB and HSV. The histograms of the color channel in the RGB color model are modified and remapped to follow the Rayleigh distribution within certain ranges. The image is then converted to the HSV color model, and the S and V components are modified within a certain limit. Qualitative and quantitative analyses indicate that the proposed method outperforms other state-of- the-art methods in terms of contrast, details, and noise reduction. The image color also shows much improvement.

  6. A redshift limit for the faint blue galaxy population from deep U band imaging

    SciTech Connect

    Guhathakurta, P.; Tyson, J.A.; Majewski, S.R. AT T Bell Laboratories, Murray Hill, NJ Yerkes Observatory, Williams Bay, WI )

    1990-07-01

    A definitive upper limit for the redshift of the population of faint blue galaxies found in deep imaging surveys is obtained. The U-B(j), and particularly the B(j)-R, colors of these objects show a blueing trend toward fainter magnitudes. A typical galaxy at R = 26 has colors that are only slightly redder than a flat spectrum. For any reasonable Lyman limit break, this constrains 93 percent or more of the galaxies to under z about 3, beyond which the break gets redshifted through the U band. The galaxies appear to be undergoing relatively recent evolution with rest frame spectra that are approximately flat down to the Lyman limit. 23 refs.

  7. Multi-view horizon-driven sea plane estimation for stereo wave imaging on moving vessels

    NASA Astrophysics Data System (ADS)

    Bergamasco, Filippo; Benetazzo, Alvise; Barbariol, Francesco; Carniel, Sandro; Sclavo, Mauro

    2016-10-01

    In the last few years we faced an increased popularity of stereo imaging as an effective tool to investigate wind sea waves at short and medium scales. Given the advances of computer vision techniques, the recovery of a scattered point-cloud from a sea surface area is nowadays a well consolidated technique producing excellent results both in terms of wave data resolution and accuracy. Nevertheless, almost all the subsequent analyses tasks, from the recovery of directional wave spectra to the estimation of significant wave height, are bound to two limiting conditions. First, wave data are required to be aligned to the mean sea plane. Second, a uniform distribution of 3D point samples is assumed. Since the stereo-camera rig is placed tilted with respect to the sea surface, perspective distortion do not allow these conditions to be met. Errors due to this problem are even more challenging if the optical instrumentation is mounted on a moving vessel, so that the mean sea plane cannot be simply obtained by averaging data from multiple subsequent frames. We address the first problem with two main contributions. First, we propose a novel horizon estimation technique to recover the attitude of a moving stereo rig with respect to the sea plane. Second, an effective weighting scheme is described to account for the non-uniform sampling of the scattered data in the estimation of the sea-plane distance. The interplay of the two allows us to provide a precise point cloud alignment without any external positioning sensor or rig viewpoint pre-calibration. The advantages of the proposed technique are evaluated throughout an experimental section spanning both synthetic and real-world scenarios.

  8. Photo-acoustic imaging of blue nanoparticle targeted brain tumor for intra-operative glioma delineation

    NASA Astrophysics Data System (ADS)

    Ray, Aniruddha; Wang, Xueding; Koo Lee, Yong-Eun; Hah, HoeJin; Kim, Gwangseong; Chen, Thomas; Orrienger, Daniel; Sagher, Oren; Kopelman, Raoul

    2011-07-01

    Distinguishing the tumor from the background neo-plastic tissue is challenging for cancer surgery such as surgical resection of glioma. Attempts have been made to use visible or fluorescent markers to delineate the tumors during surgery. However, the systemic injection of the dyes requires high dose, resulting in negative side effects. A novel method to delineate rat brain tumors intra-operatively, as well as post-operatively, using a highly sensitive photoacoustic imaging technique enhanced by tumor targeting blue nanoparticle as contrast agent is demonstrated. The nanoparticles are made of polyacrylamide (PAA) matrix with covalently linked Coomassie-Blue dye. They contain 7.0% dye and the average size is 80nm. Their surface was conjugated with F3 peptide for active tumor targeting. These nanoparticles are nontoxic, chemically inert and have long plasma circulation lifetime, making them suitable as nanodevices for imaging using photoacoustics. Experiments on phantoms and rat brains tumors ex-vivo demonstrate the high sensitivity of photoacoustic imaging in delineating the tumor, containing contrast agent at concentrations too low to be visualized by eye. The control tumors without nanoparticles did not show any enhanced signal. This study shows that photoacoustic imaging facilitated with the nanoparticle contrast agent could contribute to future surgical procedures for glioma.

  9. Characterization of the Annular Core Research Reactor (ACRR) Neutron Radiography System Imaging Plane

    NASA Astrophysics Data System (ADS)

    Kaiser, Krista; Chantel Nowlen, K.; DePriest, K. Russell

    2016-02-01

    The Annular Core Research Reactor (ACRR) at Sandia National Laboratories (SNL) is an epithermal pool-type research reactor licensed up to a thermal power of 2.4 MW. The ACRR facility has a neutron radiography facility that is used for imaging a wide range of items including reactor fuel and neutron generators. The ACRR neutron radiography system has four apertures (65:1, 125:1, 250:1, and 500:1) available to experimenters. The neutron flux and spectrum as well as the gamma dose rate were characterized at the imaging plane for the ACRR's neutron radiography system for the 65:1, 125:1 and 250:1 apertures.

  10. Vibration measurement of a miniature component by high-speed image-plane digital holographic microscopy

    SciTech Connect

    Fu Yu; Shi Hongjian; Miao Hong

    2009-04-10

    Measuring deformation of vibrating specimens whose dimensions are in the submillimeter range introduces a number of difficulties using laser interferometry. Normal interferometry is not suitable because of a phase ambiguity problem. In addition, the noise effect is much more serious in the measurement of small objects because a high-magnification lens is used. We present a method for full-field measurement of displacement, velocity, and acceleration of a vibrating miniature object based on image-plane digital holographic microscopy. A miniature cantilever beam is excited by a piezoelectric transducer stage with a sinusoidal configuration. A sequence of digital holograms is captured using a high-speed digital holographic microscope. Windowed Fourier analysis is applied in the spatial and spatiotemporal domains to extract the displacement, velocity and acceleration. The result shows that a combination of image-plane digital holographic microscopy and windowed Fourier analyses can be used to study vibration without encountering a phase ambiguity problem, and one can obtain instantaneous kinematic parameters on each point.

  11. Solid-state image sensor with focal-plane digital photon-counting pixel array

    NASA Technical Reports Server (NTRS)

    Fossum, Eric R. (Inventor); Pain, Bedabrata (Inventor)

    1995-01-01

    A photosensitive layer such as a-Si for a UV/visible wavelength band is provided for low light level imaging with at least a separate CMOS amplifier directly connected to each PIN photodetector diode to provide a focal-plane array of NxN pixels, and preferably a separate photon-counting CMOS circuit directly connected to each CMOS amplifier, although one row of counters may be time shared for reading out the photon flux rate of each diode in the array, together with a buffer memory for storing all rows of the NxN image frame before transfer to suitable storage. All CMOS circuitry is preferably fabricated in the same silicon layer as the PIN photodetector diode for a monolithic structure, but when the wavelength band of interest requires photosensitive material different from silicon, the focal-plane array may be fabricated separately on a different semiconductor layer bump-bonded or otherwise bonded for a virtually monolithic structure with one free terminal of each diode directly connected to the input terminal of its CMOS amplifier and digital counter for integration of the photon flux rate at each photodetector of the array.

  12. IMAGE-PLANE ANALYSIS OF n-POINT-MASS LENS CRITICAL CURVES AND CAUSTICS

    SciTech Connect

    Danek, Kamil; Heyrovský, David E-mail: heyrovsky@utf.mff.cuni.cz

    2015-06-10

    The interpretation of gravitational microlensing events caused by planetary systems or multiple stars is based on the n-point-mass lens model. The first planets detected by microlensing were well described by the two-point-mass model of a star with one planet. By the end of 2014, four events involving three-point-mass lenses had been announced. Two of the lenses were stars with two planetary companions each; two were binary stars with a planet orbiting one component. While the two-point-mass model is well understood, the same cannot be said for lenses with three or more components. Even the range of possible critical-curve topologies and caustic geometries of the three-point-mass lens remains unknown. In this paper we provide new tools for mapping the critical-curve topology and caustic cusp number in the parameter space of n-point-mass lenses. We perform our analysis in the image plane of the lens. We show that all contours of the Jacobian are critical curves of re-scaled versions of the lens configuration. Utilizing this property further, we introduce the cusp curve to identify cusp-image positions on all contours simultaneously. In order to track cusp-number changes in caustic metamorphoses, we define the morph curve, which pinpoints the positions of metamorphosis-point images along the cusp curve. We demonstrate the usage of both curves on simple two- and three-point-mass lens examples. For the three simplest caustic metamorphoses we illustrate the local structure of the image and source planes.

  13. InstantScope: a low-cost whole slide imaging system with instant focal plane detection

    PubMed Central

    Guo, Kaikai; Liao, Jun; Bian, Zichao; Heng, Xin; Zheng, Guoan

    2015-01-01

    We report the development of a high-throughput whole slide imaging (WSI) system by adapting a cost-effective optomechanical add-on kit to existing microscopes. Inspired by the phase detection concept in professional photography, we attached two pinhole-modulated cameras at the eyepiece ports for instant focal plane detection. By adjusting the positions of the pinholes, we can effectively change the view angle for the sample, and as such, we can use the translation shift of the two pinhole-modulated images to identify the optimal focal position. By using a small pinhole size, the focal-plane-detection range is on the order of millimeter, orders of magnitude longer than the objective’s depth of field. We also show that, by analyzing the phase correlation of the pinhole-modulated images, we can determine whether the sample contains one thin section, folded sections, or multiple layers separated by certain distances – an important piece of information prior to a detailed z scan. In order to achieve system automation, we deployed a low-cost programmable robotic arm to perform sample loading and $14 stepper motors to drive the microscope stage to perform x-y scanning. Using a 20X objective lens, we can acquire a 2 gigapixel image with 14 mm by 8 mm field of view in 90 seconds. The reported platform may find applications in biomedical research, telemedicine, and digital pathology. It may also provide new insights for the development of high-content screening instruments. PMID:26417493

  14. Simulation of High-Resolution Magnetic Resonance Images on the IBM Blue Gene/L Supercomputer Using SIMRI

    DOE PAGES

    Baum, K. G.; Menezes, G.; Helguera, M.

    2011-01-01

    Medical imaging system simulators are tools that provide a means to evaluate system architecture and create artificial image sets that are appropriate for specific applications. We have modified SIMRI, a Bloch equation-based magnetic resonance image simulator, in order to successfully generate high-resolution 3D MR images of the Montreal brain phantom using Blue Gene/L systems. Results show that redistribution of the workload allows an anatomically accurate 256 3 voxel spin-echo simulation in less than 5 hours when executed on an 8192-node partition of a Blue Gene/L system.

  15. Automatic standard plane adjustment on mobile C-Arm CT images of the calcaneus using atlas-based feature registration

    NASA Astrophysics Data System (ADS)

    Brehler, Michael; Görres, Joseph; Wolf, Ivo; Franke, Jochen; von Recum, Jan; Grützner, Paul A.; Meinzer, Hans-Peter; Nabers, Diana

    2014-03-01

    Intraarticular fractures of the calcaneus are routinely treated by open reduction and internal fixation followed by intraoperative imaging to validate the repositioning of bone fragments. C-Arm CT offers surgeons the possibility to directly verify the alignment of the fracture parts in 3D. Although the device provides more mobility, there is no sufficient information about the device-to-patient orientation for standard plane reconstruction. Hence, physicians have to manually align the image planes in a position that intersects with the articular surfaces. This can be a time-consuming step and imprecise adjustments lead to diagnostic errors. We address this issue by introducing novel semi-/automatic methods for adjustment of the standard planes on mobile C-Arm CT images. With the semi-automatic method, physicians can quickly adjust the planes by setting six points based on anatomical landmarks. The automatic method reconstructs the standard planes in two steps, first SURF keypoints (2D and newly introduced pseudo-3D) are generated for each image slice; secondly, these features are registered to an atlas point set and the parameters of the image planes are transformed accordingly. The accuracy of our method was evaluated on 51 mobile C-Arm CT images from clinical routine with manually adjusted standard planes by three physicians of different expertise. The average time of the experts (46s) deviated from the intermediate user (55s) by 9 seconds. By applying 2D SURF key points 88% of the articular surfaces were intersected correctly by the transformed standard planes with a calculation time of 10 seconds. The pseudo-3D features performed even better with 91% and 8 seconds.

  16. A Low-Cost Demonstration Kit for Locating an Image Formed by a Plane Mirror Integrated with a Ray Diagram

    ERIC Educational Resources Information Center

    Kaewkhong, Kreetha; Chitaree, Ratchapak

    2015-01-01

    This article introduces a low-cost, easy to make apparatus that can be used to locate the position of an image formed by a plane mirror. The apparatus is combined with a method used to identify an image's position by drawing a ray diagram, based on the principle of reflection, to show how an image is formed. An image's distance and an object's…

  17. Fast color flow mode imaging using plane wave excitation and temporal encoding

    NASA Astrophysics Data System (ADS)

    Udesen, Jesper; Gran, Fredrik; Jensen, Jorgen A.

    2005-04-01

    In conventional ultrasound color flow mode imaging, a large number (~500) of pulses have to be emitted in order to form a complete velocity map. This lowers the frame-rate and temporal resolution. A method for color flow imaging in which a few (~10) pulses have to be emitted to form a complete velocity image is presented. The method is based on using a plane wave excitation with temporal encoding to compensate for the decreased SNR, resulting from the lack of focusing. The temporal encoding is done with a linear frequency modulated signal. To decrease lateral sidelobes, a Tukey window is used as apodization on the transmitting aperture. The data are beamformed along the direction of the flow, and the velocity is found by 1-D cross correlation of these data. First the method is evaluated in simulations using the Field II program. Secondly, the method is evaluated using the experimental scanner RASMUS and a 7 MHz linear array transducer, which scans a circulating flowrig. The velocity of the blood mimicking fluid in the flowrig is constant and parabolic, and the center of the scanned area is situated at a depth of 40 mm. A CFM image of the blood flow in the flowrig is estimated from two pulse emissions. At the axial center line of the CFM image, the velocity is estimated over the vessel with a mean relative standard deviation of 2.64% and a mean relative bias of 6.91%. At an axial line 5 mm to the right of the center of the CFM image, the velocity is estimated over the vessel with a relative standard deviation of 0.84% and a relative bias of 5.74%. Finally the method is tested on the common carotid artery of a healthy 33-year-old male.

  18. Fractal scaling of apparent soil moisture estimated from vertical planes of Vertisol pit images

    NASA Astrophysics Data System (ADS)

    Cumbrera, Ramiro; Tarquis, Ana M.; Gascó, Gabriel; Millán, Humberto

    2012-07-01

    SummaryImage analysis could be a useful tool for investigating the spatial patterns of apparent soil moisture at multiple resolutions. The objectives of the present work were (i) to define apparent soil moisture patterns from vertical planes of Vertisol pit images and (ii) to describe the scaling of apparent soil moisture distribution using fractal parameters. Twelve soil pits (0.70 m long × 0.60 m width × 0.30 m depth) were excavated on a bare Mazic Pellic Vertisol. Six of them were excavated in April/2011 and six pits were established in May/2011 after 3 days of a moderate rainfall event. Digital photographs were taken from each Vertisol pit using a Kodak™ digital camera. The mean image size was 1600 × 945 pixels with one physical pixel ≈373 μm of the photographed soil pit. Each soil image was analyzed using two fractal scaling exponents, box counting (capacity) dimension (DBC) and interface fractal dimension (Di), and three prefractal scaling coefficients, the total number of boxes intercepting the foreground pattern at a unit scale (A), fractal lacunarity at the unit scale (Λ1) and Shannon entropy at the unit scale (S1). All the scaling parameters identified significant differences between both sets of spatial patterns. Fractal lacunarity was the best discriminator between apparent soil moisture patterns. Soil image interpretation with fractal exponents and prefractal coefficients can be incorporated within a site-specific agriculture toolbox. While fractal exponents convey information on space filling characteristics of the pattern, prefractal coefficients represent the investigated soil property as seen through a higher resolution microscope. In spite of some computational and practical limitations, image analysis of apparent soil moisture patterns could be used in connection with traditional soil moisture sampling, which always renders punctual estimates.

  19. Fourier plane colorimetric sensing using broadband imaging of surface plasmons and application to biosensing

    SciTech Connect

    Arora, P.; Krishnan, A.

    2015-12-21

    We demonstrate an optical technique for refractive index and thickness sensing of sub-wavelength-thick dielectric analytes. The technique utilizes the broadband, multimode, directional leakage radiation arising from the excitation of hybrid mode surface plasmons (SP) on low aspect ratio periodic plasmonic substrates with period ≈λ. The approach requires relaxed fabrication tolerances compared to extra ordinary transmission-based sensing techniques, wherein minor shifts in the fabricated dimensions result in a very large change from the designed resonant wavelength. We show that refractive index perturbations due to about 10-nm-thick dielectric can be captured optically by the usage of carefully designed plasmonic substrates, a halogen lamp source, free-space optical components, polarizers, and a low-end, consumer-grade charge coupled device camera. The plasmonic substrates were designed for converting the signature of hybrid mode SP excitation into a transmission peak by utilizing a thin homogeneous metal layer sandwiched between the periodic plasmonic structures and the substrate. The resonance is highly sensitive to the refractive index and thickness of the analyte superstrate. The excitation of hybrid mode SP results in a polarization rotation of 90° of the leaked radiation at resonant wavelength. In order to eliminate the problem of image registration (i.e., placing the same feature in the same pixel of the image, for comparison before and after a change in refractive index) for sensing, we perform the color analysis in the Fourier plane. The change in color of the bright emitted spot with highest momentum, corresponding to the leakage of fundamental SP mode, was used to measure the changes in refractive index, whereas the number and color of spots of lower momenta, corresponding to higher-order Fabry Perot modes, was used to measure the variation in thickness. We further show that the Fourier plane analysis can also be used to sense the index of thicker

  20. Fourier plane colorimetric sensing using broadband imaging of surface plasmons and application to biosensing

    NASA Astrophysics Data System (ADS)

    Arora, P.; Krishnan, A.

    2015-12-01

    We demonstrate an optical technique for refractive index and thickness sensing of sub-wavelength-thick dielectric analytes. The technique utilizes the broadband, multimode, directional leakage radiation arising from the excitation of hybrid mode surface plasmons (SP) on low aspect ratio periodic plasmonic substrates with period ≈λ. The approach requires relaxed fabrication tolerances compared to extra ordinary transmission-based sensing techniques, wherein minor shifts in the fabricated dimensions result in a very large change from the designed resonant wavelength. We show that refractive index perturbations due to about 10-nm-thick dielectric can be captured optically by the usage of carefully designed plasmonic substrates, a halogen lamp source, free-space optical components, polarizers, and a low-end, consumer-grade charge coupled device camera. The plasmonic substrates were designed for converting the signature of hybrid mode SP excitation into a transmission peak by utilizing a thin homogeneous metal layer sandwiched between the periodic plasmonic structures and the substrate. The resonance is highly sensitive to the refractive index and thickness of the analyte superstrate. The excitation of hybrid mode SP results in a polarization rotation of 90° of the leaked radiation at resonant wavelength. In order to eliminate the problem of image registration (i.e., placing the same feature in the same pixel of the image, for comparison before and after a change in refractive index) for sensing, we perform the color analysis in the Fourier plane. The change in color of the bright emitted spot with highest momentum, corresponding to the leakage of fundamental SP mode, was used to measure the changes in refractive index, whereas the number and color of spots of lower momenta, corresponding to higher-order Fabry Perot modes, was used to measure the variation in thickness. We further show that the Fourier plane analysis can also be used to sense the index of thicker

  1. Highly sensitive detection of the soft tissues based on refraction contrast by in-plane diffraction-enhanced imaging CT

    NASA Astrophysics Data System (ADS)

    Yuasa, Tetsuya; Hashimoto, Eiko; Maksimenko, Anton; Sugiyama, Hiroshi; Arai, Yoshinori; Shimao, Daisuke; Ichihara, Shu; Ando, Masami

    2008-07-01

    We discuss the recently proposed computed tomography (CT) technique based on refractive effects for biomedical use, which reconstructs the in-plane refractive-index gradient vector field in a cross-sectional plane of interest by detecting the angular deviation of the beam, refracted by a sample, from the incident beam, using the diffraction-enhanced imaging (DEI) method. The CT has advantages for delineating biological weakly absorbing soft tissues over the conventional absorption-contrast CT because of the use of phase sensitive detection. The paper aims to define the imaging scheme rigidly and to demonstrate its efficacy for non-destructive measurement of biomedical soft-tissue samples without imaging agent. We first describe the imaging principle of in-plane DEI-CT from the physico-mathematical viewpoints in detail, and investigate what physical quantities are extracted from the reconstructed images. Then, we introduce the imaging system using the synchrotron radiation as a light source, constructed at beamline BL-14B in KEK, Japan. Finally, we demonstrate the advantage of the refraction-based image for non-destructive analysis of biological sample by investigating the image of human breast cancer tumors obtained using the imaging system. Here, the refraction- and the apparent absorption-based images obtained simultaneously by the in-plane DEI-CT are compared. Also, the conventional absorption-based image obtained using micro-computed tomography (μCT) imaging system is compared with them. Thereby, it is shown that the refraction contrast much more sensitively delineates the soft tissues than the absorption contrast. In addition, the radiologic-histologic correlation study not only validates the efficacy for imaging soft tissues, but also produces the potential that the pathological inspection for the breast cancer tumors may be feasible non-destructively.

  2. LOOKING THROUGH THE GALACTIC PLANE: IMAGING COLD DUST TOWARD l = 44 DEG

    SciTech Connect

    Matthews, Henry; Kirk, Helen; Johnstone, Doug; Weferling, Bernd; Cohen, Martin; Jenness, Tim; Davis, Gary; Evans, Aneurin; Dent, William R. F.; Fuller, Gary; Jackson, James M.; Rathborne, Jill; Richer, John; Simon, Robert

    2009-11-15

    We present imaging observations of continuum emission from interstellar dust at 850 and 1200 {mu}m of a section of the Galactic Plane covering 2 deg{sup 2} centered at l = 44 DEG. Complementary jiggle-mapping and fast-scanning techniques were used, respectively, at these two wavelengths. The mapped area includes the well-known star formation regions W49 and G45.1/45.5. Using an automated clump-finding routine, we identify 132 compact 850 {mu}m emission features within the region above a completeness level of about 200 mJy beam{sup -1}. The positions of the latter objects were used to determine fluxes from the 1200 {mu}m image. Spectral line data were subsequently obtained with the same observing beamwidth as at 850 {mu}m for almost half of the objects; these were either imaged in the {sup 13}CO (3-2) line, or basic characteristics determined using the {sup 12}CO (3-2) transition. We use these data, supplemented by existing {sup 13}CO (1-0) and H I survey data, to determine distances and hence derive masses for the dust clump ensemble, assuming a uniform dust temperature of 15 K. From these data we find that the number-mass relationship for clumps in the field is similar to that found for individual star-forming regions.

  3. Out-of-plane Stokes imaging polarimeter for early skin cancer diagnosis

    NASA Astrophysics Data System (ADS)

    Ghassemi, Pejhman; Lemaillet, Paul; Germer, Thomas A.; Shupp, Jeffrey W.; Venna, Suraj S.; Boisvert, Marc E.; Flanagan, Katherine E.; Jordan, Marion H.; Ramella-Roman, Jessica C.

    2012-07-01

    Optimal treatment of skin cancer before it metastasizes critically depends on early diagnosis and treatment. Imaging spectroscopy and polarized remittance have been utilized in the past for diagnostic purposes, but valuable information can be also obtained from the analysis of skin roughness. For this purpose, we have developed an out-of-plane hemispherical Stokes imaging polarimeter designed to monitor potential skin neoplasia based on a roughness assessment of the epidermis. The system was utilized to study the rough surface scattering for wax samples and human skin. The scattering by rough skin--simulating phantoms showed behavior that is reasonably described by a facet scattering model. Clinical tests were conducted on patients grouped as follows: benign nevi, melanocytic nevus, melanoma, and normal skin. Images were captured and analyzed, and polarization properties are presented in terms of the principal angle of the polarization ellipse and the degree of polarization. In the former case, there is separation between different groups of patients for some incidence azimuth angles. In the latter, separation between different skin samples for various incidence azimuth angles is observed.

  4. CCD Centroiding Experiment for Correcting a Distorted Image on the Focal Plane

    NASA Astrophysics Data System (ADS)

    Yano, Taihei; Araki, Hiroshi; Gouda, Naoteru; Kobayashi, Yukiyasu; Tsujimoto, Takuji; Nakajima, Tadashi; Kawano, Nobuyuki; Tazawa, Seiichi; Yamada, Yoshiyuki; Hanada, Hideo; Asari, Kazuyoshi; Tsuruta, Seiitsu

    2006-10-01

    JASMINE (Japan Astrometry Satellite Mission for Infrared Exploration) and ILOM (In situ Lunar Orientation Measurement) are space missions that are in progress at the National Astronomical Observatory of Japan. These two projects require a common astrometric technique to obtain precise positions of star images on solid-state detectors in order to accomplish their objectives. In the laboratory, we have carried out measurements of the centroid of artificial star images on a CCD array in order to investigate the precision of the positions of the stars, using an algorithm for estimating them from photon-weighted means of the stars. In the calibration of the position of a star image at the focal plane, we have also taken into account the lowest order distortion due to optical aberrations, which is proportional to the cube of the distance from the optical axis. Accordingly, we find that the precision of the measurement for the positions of the stars reaches below 1/100 pixel for one measurement.

  5. Solid-state Image Sensor with Focal-plane Digital Photon-counting Pixel Array

    NASA Technical Reports Server (NTRS)

    Fossum, Eric R.; Pain, Bedabrata

    1997-01-01

    A solid-state focal-plane imaging system comprises an NxN array of high gain. low-noise unit cells. each unit cell being connected to a different one of photovoltaic detector diodes, one for each unit cell, interspersed in the array for ultra low level image detection and a plurality of digital counters coupled to the outputs of the unit cell by a multiplexer(either a separate counter for each unit cell or a row of N of counters time shared with N rows of digital counters). Each unit cell includes two self-biasing cascode amplifiers in cascade for a high charge-to-voltage conversion gain (greater than 1mV/e(-)) and an electronic switch to reset input capacitance to a reference potential in order to be able to discriminate detection of an incident photon by the photoelectron (e(-))generated in the detector diode at the input of the first cascode amplifier in order to count incident photons individually in a digital counter connected to the output of the second cascade amplifier. Reseting the input capacitance and initiating self-biasing of the amplifiers occurs every clock cycle of an integratng period to enable ultralow light level image detection by the may of photovoltaic detector diodes under such ultralow light level conditions that the photon flux will statistically provide only a single photon at a time incident on anyone detector diode during any clock cycle.

  6. Sentinel lymph nodes detection with an imaging system using Patent Blue V dye as fluorescent tracer

    NASA Astrophysics Data System (ADS)

    Tellier, F.; Steibel, J.; Chabrier, R.; Rodier, J. F.; Pourroy, G.; Poulet, P.

    2013-03-01

    Sentinel lymph node biopsy is the gold standard to detect metastatic invasion from primary breast cancer. This method can help patients avoid full axillary chain dissection, thereby decreasing the risk of morbidity. We propose an alternative to the traditional isotopic method, to detect and map the sentinel lymph nodes. Indeed, Patent Blue V is the most widely used dye in clinical routine for the visual detection of sentinel lymph nodes. A Recent study has shown the possibility of increasing the fluorescence quantum yield of Patent Blue V, when it is bound to human serum albumin. In this study we present a preclinical fluorescence imaging system to detect sentinel lymph nodes labeled with this fluorescent tracer. The setup is composed of a black and white CCD camera and two laser sources. One excitation source with a laser emitting at 635 nm and a second laser at 785 nm to illuminate the region of interest. The prototype is operated via a laptop. Preliminary experiments permitted to determine the device sensitivity in the μmol.L-1 range as regards the detection of PBV fluorescence signals. We also present a preclinical evaluation performed on Lewis rats, during which the fluorescence imaging setup detected the accumulation and fixation of the fluorescent dye on different nodes through the skin.

  7. Fluorescence imaging of Evans blue extravasation into mouse brain induced by low frequency ultrasound with microbubble.

    PubMed

    Shen, Yuanyuan; Zhang, Aili; Guo, Jinxuan; Dan, Guo; Chen, Siping; Yu, Hao

    2014-01-01

    Blood-Brain Barrier (BBB) can be opened locally, noninvasively and reversibly by low frequency focused ultra-sound (FUS) in the presence of microbubbles. In this study, Evans blue (EB) dye extravasation across BBB was enhanced by 1 MHz FUS at acoustic pressure of 0.35 MPa in the presence of microbubbles at clinically comparable dosage. The spatial distribution of EB extravasation was visualized using fluorescence imaging method. The center region of BBB disruption area showed more enhanced fluorescence signal than the surrounding region in general. However, EB dye deposition was heterogeneous in the center region. The findings in this study indicated potential use of fluorescence imaging to evaluate large molecules delivery across BBB.

  8. Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin

    PubMed Central

    Tellier, Franklin; Steibel, Jérôme; Chabrier, Renée; Blé, François Xavier; Tubaldo, Hervé; Rasata, Ravelo; Chambron, Jacques; Duportail, Guy; Simon, Hervé; Rodier, Jean-François; Poulet, Patrick

    2012-01-01

    Patent Blue V (PBV), a dye used clinically for sentinel lymph node detection, was mixed with human serum albumin (HSA). After binding to HSA, the fluorescence quantum yield increased from 5 × 10−4 to 1.7 × 10−2, which was enough to allow fluorescence detection and imaging of its distribution. A detection threshold, evaluated in scattering test objects, lower than 2.5 nmol × L−1 was obtained, using a single-probe setup with a 5-mW incident light power. The detection sensitivity using a fluorescence imaging device was in the µmol × L−1 range, with a noncooled CCD camera. Preclinical evaluation was performed on a rat model and permitted to observe inflamed nodes on all animals. PMID:23024922

  9. Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin.

    PubMed

    Tellier, Franklin; Steibel, Jérôme; Chabrier, Renée; Blé, François Xavier; Tubaldo, Hervé; Rasata, Ravelo; Chambron, Jacques; Duportail, Guy; Simon, Hervé; Rodier, Jean-François; Poulet, Patrick

    2012-09-01

    Patent Blue V (PBV), a dye used clinically for sentinel lymph node detection, was mixed with human serum albumin (HSA). After binding to HSA, the fluorescence quantum yield increased from 5 × 10(-4) to 1.7 × 10(-2), which was enough to allow fluorescence detection and imaging of its distribution. A detection threshold, evaluated in scattering test objects, lower than 2.5 nmol × L(-1) was obtained, using a single-probe setup with a 5-mW incident light power. The detection sensitivity using a fluorescence imaging device was in the µmol × L(-1) range, with a noncooled CCD camera. Preclinical evaluation was performed on a rat model and permitted to observe inflamed nodes on all animals.

  10. Research on auto-calibration technology of the image plane's center of 360-degree and all round looking camera

    NASA Astrophysics Data System (ADS)

    Zhang, Shaojun; Xu, Xiping

    2015-10-01

    The 360-degree and all round looking camera, as its characteristics of suitable for automatic analysis and judgment on the ambient environment of the carrier by image recognition algorithm, is usually applied to opto-electronic radar of robots and smart cars. In order to ensure the stability and consistency of image processing results of mass production, it is necessary to make sure the centers of image planes of different cameras are coincident, which requires to calibrate the position of the image plane's center. The traditional mechanical calibration method and electronic adjusting mode of inputting the offsets manually, both exist the problem of relying on human eyes, inefficiency and large range of error distribution. In this paper, an approach of auto- calibration of the image plane of this camera is presented. The imaging of the 360-degree and all round looking camera is a ring-shaped image consisting of two concentric circles, the center of the image is a smaller circle and the outside is a bigger circle. The realization of the technology is just to exploit the above characteristics. Recognizing the two circles through HOUGH TRANSFORM algorithm and calculating the center position, we can get the accurate center of image, that the deviation of the central location of the optic axis and image sensor. The program will set up the image sensor chip through I2C bus automatically, we can adjusting the center of the image plane automatically and accurately. The technique has been applied to practice, promotes productivity and guarantees the consistent quality of products.

  11. Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics.

    PubMed

    Duocastella, Martí; Sun, Bo; Arnold, Craig B

    2012-05-01

    Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.

  12. Blue Laser Imaging-Bright Improves Endoscopic Recognition of Superficial Esophageal Squamous Cell Carcinoma

    PubMed Central

    Tomie, Akira; Yagi, Nobuaki; Kitae, Hiroaki; Majima, Atsushi; Horii, Yusuke; Kitaichi, Tomoko; Onozawa, Yuriko; Suzuki, Kentaro; Kimura-Tsuchiya, Reiko; Okayama, Tetsuya; Kamada, Kazuhiro; Katada, Kazuhiro; Uchiyama, Kazuhiko; Ishikawa, Takeshi; Takagi, Tomohisa; Naito, Yuji; Itoh, Yoshito

    2016-01-01

    Background/Aims. The aim of this study was to evaluate the endoscopic recognition of esophageal squamous cell carcinoma (ESCC) using four different methods (Olympus white light imaging (O-WLI), Fujifilm white light imaging (F-WLI), narrow band imaging (NBI), and blue laser imaging- (BLI-) bright). Methods. We retrospectively analyzed 25 superficial ESCCs that had been examined using the four different methods. Subjective evaluation was provided by three endoscopists as a ranking score (RS) of each image based on the ease of detection of the cancerous area. For the objective evaluation we calculated the color difference scores (CDS) between the cancerous and noncancerous areas with each of the four methods. Results. There was no difference between the mean RS of O-WLI and F-WLI. The mean RS of NBI was significantly higher than that of O-WLI and that of BLI-bright was significantly higher than that of F-WLI. Moreover, the mean RS of BLI-bright was significantly higher than that of NBI. Furthermore, in the objective evaluation, the mean CDS of BLI-bright was significantly higher than that of O-WLI, F-WLI, and NBI. Conclusion. The recognition of superficial ESCC using BLI-bright was more efficacious than the other methods tested both subjectively and objectively. PMID:27738428

  13. Comparative evaluation of methylene blue and demeclocycline for enhancing optical contrast of gliomas in optical images

    NASA Astrophysics Data System (ADS)

    Wirth, Dennis; Snuderl, Matija; Curry, William; Yaroslavsky, Anna

    2014-09-01

    Contrast agents have shown to be useful in the detection of cancers. The goal of this study was to compare enhancement of brain cancer contrast using reflectance and fluorescence confocal imaging of two fluorophores, methylene blue (MB) and demeclocycline (DMN). MB absorbs light in the red spectral range and fluoresces in the near-infrared. It is safe for in vivo staining of human skin and breast tissue. However, its safety for staining human brain is questionable. Thus, DMN, which absorbs light in the violet spectral range and fluoresces between 470 and 570 nm, could provide a safer alternative to MB. Fresh human gliomas, obtained from surgeries, were cut in half and stained with aqueous solutions of MB and DMN, respectively. Stained tissues were imaged using multimodal confocal microscopy. Resulting reflectance and fluorescence optical images were compared with hematoxylin and eosin histopathology, processed from each imaged tissue. Results indicate that images of tissues stained with either stain exhibit comparable contrast and resolution of morphological detail. Further studies are required to establish the safety and efficacy of these contrast agents for use in human brain.

  14. Massively-parallel electrical-conductivity imaging of hydrocarbonsusing the Blue Gene/L supercomputer

    SciTech Connect

    Commer, M.; Newman, G.A.; Carazzone, J.J.; Dickens, T.A.; Green,K.E.; Wahrmund, L.A.; Willen, D.E.; Shiu, J.

    2007-05-16

    Large-scale controlled source electromagnetic (CSEM)three-dimensional (3D) geophysical imaging is now receiving considerableattention for electrical conductivity mapping of potential offshore oiland gas reservoirs. To cope with the typically large computationalrequirements of the 3D CSEM imaging problem, our strategies exploitcomputational parallelism and optimized finite-difference meshing. Wereport on an imaging experiment, utilizing 32,768 tasks/processors on theIBM Watson Research Blue Gene/L (BG/L) supercomputer. Over a 24-hourperiod, we were able to image a large scale marine CSEM field data setthat previously required over four months of computing time ondistributed clusters utilizing 1024 tasks on an Infiniband fabric. Thetotal initial data misfit could be decreased by 67 percent within 72completed inversion iterations, indicating an electrically resistiveregion in the southern survey area below a depth of 1500 m below theseafloor. The major part of the residual misfit stems from transmitterparallel receiver components that have an offset from the transmittersail line (broadside configuration). Modeling confirms that improvedbroadside data fits can be achieved by considering anisotropic electricalconductivities. While delivering a satisfactory gross scale image for thedepths of interest, the experiment provides important evidence for thenecessity of discriminating between horizontal and verticalconductivities for maximally consistent 3D CSEM inversions.

  15. Experimental study on the push-broom infrared imaging system based on line-plane-switching fiber bundle

    NASA Astrophysics Data System (ADS)

    Yan, Xingtao; Li, Fu; Ma, Xiaolong; Lv, Juan; He, Yinghong; Zhao, Yiyi; Bu, Fan

    2016-10-01

    The use of line-plane-switching infrared fiber bundle to achieve wide field of view push-broom infrared imaging has been studied with experiment. In this technology, the linear array end of the imaging fiber bundle is used as a long-linear array infrared detector, and the plane array end of the bundle is coupled by a mature small scale Infrared Focal Plane Array (IRFPA). It can evade the difficulty of getting the long-linear array infrared detector directly, and has a signally significance to the development of internal infrared imaging technology. Based on the introduction of the composition, working principle of this novel infrared optical system, the system principle-demonstrating experiment has been accomplished. The line-plane-switching fiber bundle used in this experiment is 64×9 format plane array and 192×3 format linear array. It is made from chalcogenide glass fibers, possessing core (As40S59.5Se0.5) of 45 μm, cladding (As40S60) of 5 μm, and error of 1% in diameter. Perfect imaging results prove that this novel technology is feasibility and superiority. The analysis of the experiment makes a foundation for the subsequent further verification experiments.

  16. Robust angle-independent blood velocity estimation based on dual-angle plane wave imaging.

    PubMed

    Fadnes, Solveig; Ekroll, Ingvild Kinn; Nyrnes, Siri Ann; Torp, Hans; Lovstakken, Lasse

    2015-10-01

    Two-dimensional blood velocity estimation has shown potential to solve the angle-dependency of conventional ultrasound flow imaging. Clutter filtering, however, remains a major challenge for large beam-to-flow angles, leading to signal drop-outs and corrupted velocity estimates. This work presents and evaluates a compounding speckle tracking (ST) algorithm to obtain robust angle-independent 2-D blood velocity estimates for all beam-to-flow angles. A dual-angle plane wave imaging setup with full parallel receive beamforming is utilized to achieve high-frame-rate speckle tracking estimates from two scan angles, which may be compounded to obtain velocity estimates of increased robustness. The acquisition also allows direct comparison with vector Doppler (VD) imaging. Absolute velocity bias and root-mean-square (RMS) error of the compounding ST estimations were investigated using simulations of a rotating flow phantom with low velocities ranging from 0 to 20 cm/s. In a challenging region where the estimates were influenced by clutter filtering, the bias and RMS error for the compounding ST estimates were 11% and 2 cm/s, a significant reduction compared with conventional single-angle ST (22% and 4 cm/s) and VD (36% and 6 cm/s). The method was also tested in vivo for vascular and neonatal cardiac imaging. In a carotid artery bifurcation, the obtained blood velocity estimates showed that the compounded ST method was less influenced by clutter filtering than conventional ST and VD methods. In the cardiac case, it was observed that ST velocity estimation is more affected by low signal-to-noise (SNR) than VD. However, with sufficient SNR the in vivo results indicated that a more robust angle-independent blood velocity estimator is obtained using compounded speckle tracking compared with conventional ST and VD methods.

  17. Portable sequential multicolor thermal imager based on a MCT 384 x 288 focal plane array

    NASA Astrophysics Data System (ADS)

    Breiter, Rainer; Cabanski, Wolfgang A.; Mauk, Karl-Heinz; Rode, Werner; Ziegler, Johann

    2001-10-01

    AIM has developed a sequential multicolor thermal imager to provide customers with a test system to realize real-time spectral selective thermal imaging. In contrast to existing PC based laboratory units, the system is miniaturized with integrated signal processing like non-uniformity correction and post processing functions such as image subtraction of different colors to allow field tests in military applications like detection of missile plumes or camouflaged targets as well as commercial applications like detection of chemical agents, pollution control, etc. The detection module used is a 384 X 288 mercury cadmium telluride (MCT) focal plane array (FPA) available in the mid wave (MWIR) or long wave spectral band LWIR). A compact command and control electronics (CCE) provides clock and voltage supply for the detector as well as 14 bit deep digital conversion of the analog detector output. A continuous rotating wheel with four facets for filters provides spectral selectivity. The customer can choose between various types of filter characteristics, e.g. a 4.2 micrometer bandpass filter for CO2 detection in the MWIR band. The rotating wheel can be synchronized to an external source giving the rotation speed, typical 25 l/s. A position sensor generates the four frame start signals for synchronous operation of the detector -- 100 Hz framerate for the four frames per rotation. The rotating wheel is exchangeable for different configurations and also plates for a microscanner operation to improve geometrical resolution are available instead of a multicolor operation. AIM's programmable MVIP image processing unit is used for signal processing like non- uniformity correction and controlling the detector parameters. The MVIP allows to output the four subsequent images as four quarters of the video screen to prior to any observation task set the integration time for each color individually for comparable performance in each spectral color and after that also to determine

  18. Band-limited image plane masks for the Terrestrial Planet Finder coronagraph: materials and designs for broadband performance.

    PubMed

    Balasubramanian, Kunjithapatham

    2008-01-10

    Coronagraphs for detection and characterization of exosolar earthlike planets require accurate masks with broadband performance in the visible and near infrared spectrum. Design and fabrication of image plane masks capable of suppressing broadband starlight to 10(-10) level contrast presents technical challenges. We discuss basic approaches, material choices, designs, and fabrication options for image plane masks with particular focus on material properties to obtain adequate spectral performance. Based on theoretical analysis, we show that metals such as Pt and Ni, and alloys such as Inconel, may be employed as promising mask materials that can meet broadband performance requirements.

  19. NOTE: Design and fabrication of a high sensitivity focal plane array for uncooled IR imaging

    NASA Astrophysics Data System (ADS)

    Yu, Xiaomei; Yi, Yuliang; Ma, Shenglin; Liu, Ming; Liu, Xiaohua; Dong, Liquan; Zhao, Yuejin

    2008-05-01

    This note reports on the development of a novel cantilever-based focal plane array (FPA) for uncooled infrared (IR) imaging. The FPA of 160 × 120 pixels consisted of a 1 µm thick low stress SiNx structure layer, a thin gold reflection layer and a thick gold bimaterial layer. A bulk silicon process that includes silicon-glass anodic bonding and deep reactive ion etching techniques was developed selectively to remove the substrate silicon and form silicon frames for every FPA pixel. The thermomechanical sensitivity of the cantilever pixel was measured as 0.11 µm K-1, the noise-equivalent temperature difference of the FPA was theoretically estimated to be below 60 mK and the response time was calculated to be 15 ms. An optical readout system was used to measure deflections of all cantilevers in the FPA simultaneously, and thermal images of the human body were captured in good time. One of the unique advantages of this honeycomb-like FPA is the selective removal of the silicon substrate, which could increase the IR absorption efficiency by 48% compared with that fabricated by a traditional surface sacrificial layer process.

  20. Pupil plane interferometry in the near infrared. II - Phase recovery and image reconstruction

    NASA Astrophysics Data System (ADS)

    Monin, J.-L.; Mariotti, J.-M.; Ghez, P.; Perrier, C.; Desbat, L.

    1992-07-01

    We describe a 2D method to extract the phase of turbulence-blurred near-IR interferograms in the plane of the telescope pupil. The instrument and its principle have been described by Mariotti et al. (1992). The phase data are obtained from near-IR interference fringes produced by a Michelson interferometer in the image of the telescope pupil. We have developed an equivalent of the Knox-Thompson algorithm, using the spatial correlation of the phase over the pupil to recover the actual 2D phase of the complex visibility function. We compute and average the spatial gradients of the instantaneous phase, and a minimal norm least squares method is then used to recover the actual phase over the pupil. The modulus and the phase can be used to recover diffraction-limited images. We present an application of the method on a simulated binary system, together with first results on some astronomical data recently obtained at the 4.20-m William Herschel Telescope of the Royal Greenwich Observatory at La Palma. Tentative deconvolutions are presented.

  1. Comparative analysis on viewing angle change in Fresnel and Fourier holographic images reconstructed by a tilted plane wave.

    PubMed

    Chae, Byung Gyu

    2014-05-20

    We carry out a comparative analysis on a viewing angle change in Fresnel and Fourier holographic images reconstructed by a tilted plane wave. A tilted plane wave illuminating an on-axis hologram generates a diffractive wave carrying the holographic image in a paraxial region of a new diffraction axis. The reconstructed image in the Fresnel hologram is deformed along the new viewing direction, which is well described as Affine transformation. In the Fourier holographic image, the replica of the image is formed without its deformation when the hologram is placed in the front focal plane of the lens, whereas in the case of a hologram that is located at a distance different from a focal length, image deformation arises. This property is investigated through numerical simulation based on a wide-angle diffraction phenomenon. We also perform a similar interpretation for high-order diffraction images appearing in the sampled Fourier hologram and discuss a method for enlarging the viewing angle of the holographic image.

  2. Forensic discrimination of blue ballpoint pen inks based on thin layer chromatography and image analysis.

    PubMed

    Djozan, Djavanshir; Baheri, Tahmineh; Karimian, Ghader; Shahidi, Masomeh

    2008-08-06

    This article aims to provide a new and fast method for differentiation of inks on a questioned document. The data acquisition was carried out by designing specific image analysis software for evaluating thin layer chromatograms (TLC-IA). The ink spot was extracted from the document using methanol and separated by TLC using plastic sheet silica gel 60 without fluorescent indicator, and a mixture of ethyl acetate, ethanol, and water (70:35:30, v/v/v) as mobile phase. To discriminate between different pen inks, new software was designed on the basis of intensity profile of red, green, and blue (RGB) characteristic. In practice, after development of chromatogram, the chromatograms were scanned by ordinary office scanner, intensity profiles of RGB characteristics on the development straight of each sample were produced and compared with the mentioned software. RGB profiles of ballpoint inks from various manufacturers showed that the patterns in most cases were distinctly different from each other. This new method allowed discriminating among different pen inks with a high reliability and the discriminating power of 92.8%. Blue ballpoint pen inks of 41 different samples available on the local market were successfully analyzed and discriminated.

  3. Least-Squares Multi-Angle Doppler Estimators for Plane Wave Vector Flow Imaging.

    PubMed

    Yiu, Billy Y S; Yu, Alfred C H

    2016-06-20

    Designing robust Doppler vector estimation strategies for use in plane wave imaging schemes based on unfocused transmissions is a topic that has yet to be studied in depth. One potential solution is to use a multi-angle Doppler estimation approach that computes flow vectors via least-squares fitting, but its performance has not been established. Here, we investigated the efficacy of multi-angle Doppler vector estimators by: (i) comparing its performance with respect to the classical dual-angle (cross-beam) Doppler vector estimator; (ii) examining the working effects of multi-angle Doppler vector estimators on flow visualization quality in the context of dynamic flow path rendering. Implementing Doppler vector estimators that use different combinations of transmit (Tx) and receive (Rx) steering angles, our analysis has compared the classical dual-angle Doppler method, a 5-Tx version of dual-angle Doppler, and various multi-angle Doppler configurations based on 3 Tx and 5 Tx. Two angle spans (10°, 20°) were examined in forming the steering angles. In imaging scenarios with known flow profiles (rotating disc and straight-tube parabolic flow), the 3-Tx, 3-Rx and 5-Tx, 5-Rx multi-angle configurations produced vector estimates with smaller variability comparing to the dual-angle method, and the estimation results were more consistent with the use of a 20° angle span. Flow vectors derived from multi-angle Doppler estimators were also found to be effective in rendering the expected flow paths in both rotating disc and straight-tube imaging scenarios, while the ones derived from the dual-angle estimator yielded flow paths that deviated from the expected course. These results serve to attest that, using multi-angle least-squares Doppler vector estimators, flow visualization can be consistently achieved.

  4. Least-Squares Multi-Angle Doppler Estimators for Plane-Wave Vector Flow Imaging.

    PubMed

    Yiu, Billy Y S; Yu, Alfred C H

    2016-11-01

    Designing robust Doppler vector estimation strategies for use in plane-wave imaging schemes based on unfocused transmissions is a topic that has yet to be studied in depth. One potential solution is to use a multi-angle Doppler estimation approach that computes flow vectors via least-squares fitting, but its performance has not been established. Here, we investigated the efficacy of multi-angle Doppler vector estimators by: 1) comparing its performance with respect to the classical dual-angle (cross-beam) Doppler vector estimator and 2) examining the working effects of multi-angle Doppler vector estimators on flow visualization quality in the context of dynamic flow path rendering. Implementing Doppler vector estimators that use different combinations of transmit (Tx) and receive (Rx) steering angles, our analysis has compared the classical dual-angle Doppler method, a 5-Tx version of dual-angle Doppler, and various multi-angle Doppler configurations based on 3 Tx and 5 Tx. Two angle spans (10°, 20°) were examined in forming the steering angles. In imaging scenarios with known flow profiles (rotating disk and straight-tube parabolic flow), the 3-Tx, 3-Rx and 5-Tx, 5-Rx multi-angle configurations produced vector estimates with smaller variability compared with the dual-angle method, and the estimation results were more consistent with the use of a 20° angle span. Flow vectors derived from multi-angle Doppler estimators were also found to be effective in rendering the expected flow paths in both rotating disk and straight-tube imaging scenarios, while the ones derived from the dual-angle estimator yielded flow paths that deviated from the expected course. These results serve to attest that using multi-angle least-squares Doppler vector estimators, flow visualization can be consistently achieved.

  5. Blue-enhanced thin-film photodiode for dual-screen x-ray imaging

    SciTech Connect

    Vygranenko, Y.; Vieira, M.; Sazonov, A.; Heiler, G.; Tredwell, T.; Nathan, A.

    2009-12-28

    This article reports on a-Si:H-based low-leakage blue-enhanced photodiodes for dual-screen x-ray imaging detectors. Doped nanocrystalline silicon was incorporated in both the n- and p-type regions to reduce absorption losses for light incoming from the top and bottom screens. The photodiode exhibits a dark current density of 900 pA/cm{sup 2} and an external quantum efficiency up to 90% at a reverse bias of 5 V. In the case of illumination through the tailored p-layer, the quantum efficiency of 60% at a 400 nm wavelength is almost double that for the conventional a-Si:H n-i-p photodiode.

  6. The study of blue LED to induce fluorescence spectroscopy and fluorescence imaging for oral carcinoma detection

    NASA Astrophysics Data System (ADS)

    Zheng, Longjiang; Hu, Yuanting

    2009-07-01

    Fluorescence spectroscopy and fluorescence imaging diagnosis of malignant lesions provides us with a new method to diagnose diseases in precancerous stage. Early diagnosis of disease has significant importance in cancer treatment, because most cancers can be cured well in precancerous, especially when the diffusion of cancer is limited in a restricted region. In this study, Golden hamster models were applied to 5% 9, 10 dimethyl-1, 2-benzanthracene (DMBA) to induce hamster buccal cheek pouch carcinoma three times a week. Rose Bengal, which has been used in clinican for years and avoids visible side-effect to human was chosen as photosensitizer. 405 nm blue LED was used to induce the fluorescence of photosensitizer. After topical application of photosensitizer, characteristic red emission fluorescence peak was observed around 600nm. Similar, normal oral cavity has special luminescence around 480nm. Fluorescence spectroscopy technology is based on analysing emission peaks of photosensitizer in the areas of oral carcinoma, moreover, red-to-green (IR/IG) intensity ratio is also applied as a diagnostic algorithm. A CCD which is connected with a computer is used to take pictures at carcinoma areas through different filters. Fluorescence images from normal hamster buccal cheek pouch are compared with those from carcinogen-induced models of carcinoma, and morphological differences between normal and lesion tissue can be distinguished. The pictures are analyzed by Matlab and shown on the screen of computer. This paper demonstrates that Rose Bengal could be used as photosensitizer to detect oral carcinoma, and blue LED as excitation source could not only have a good effect to diagnose oral carcinoma, but also decrease cost greatly.

  7. Pair-Wise, Deformable Mirror, Image Plane-Based Diversity Electric Field Estimation for High Contrast Coronagraphy

    NASA Technical Reports Server (NTRS)

    Give'on, Amir; Kern, Brian D.; Shaklan, Stuart

    2011-01-01

    In this paper we describe the complex electric field reconstruction from image plane intensity measurements for high contrast coronagraphic imaging. A deformable mirror (DM) surface is modied with pairs of complementary shapes to create diversity in the image plane of the science camera where the intensity of the light is measured. Along with the Electric Field Conjugation correction algorithm, this estimation method has been used in various high contrast imaging testbeds to achieve the best contrasts to date both in narrow and in broad band light. We present the basic methodology of estimation in easy to follow list of steps, present results from HCIT and raise several open quations we are confronted with using this method.

  8. Infrared hyperspectral imaging using a broadly tunable external cavity quantum cascade laser and microbolometer focal plane array

    SciTech Connect

    Phillips, Mark C.; Ho, Nicolas

    2008-02-04

    A versatile mid-infrared hyperspectral imaging system is demonstrated by combining a broadly tunable external cavity quantum cascade laser and a microbolometer focal plane array. The tunable mid-infrared laser provided high brightness illumination over a tuning range from 985 cm-1 to 1075 cm-1 (9.30-10.15 μm). Hypercubes containing images at 300 wavelengths separated by 0.3 cm 1 were obtained in 12 s. High spectral resolution chemical imaging of methanol vapor was demonstrated for both static and dynamic systems. The system was also used to image and characterize multiple component liquid and solid samples.

  9. Predictors of Morbidity and Cleavage Plane in Surgical Resection of Pure Convexity Meningiomas Using Cerebrospinal Fluid Sensitive Image Subtraction Magnetic Resonance Imaging

    PubMed Central

    THENIER-VILLA, José LUIS; CAMPOVERDE, Raúl ALEJANDRO GALÁRRAGA; DE LA LAMA ZARAGOZA, Adolfo RAMÓN; ALONSO, Cesáreo CONDE

    2017-01-01

    Meningiomas are the most common primary intracranial tumors. Since the adhesions in the plane of dissection are of interest in surgical planning, we suggest that digital image subtraction of FLAIR data from the T2 sequence of MRI may represent better the CSF spaces in the brain–tumor interface and may be a predictor of the intraoperative cleavage plane. From 2006 to 2016, 83 convexity meningiomas were resected in the Department of Neurosurgery of the University Hospital Complex of Vigo, an analysis of preoperative MRI was performed to assess peritumoral edema (PTE), tumor volume, among others; a digital subtraction of T2-FLAIR sequences was performed and analyzed in relationship to the cleavage plane described in the intraoperative report and postoperative neurological deficits. Simpson grade 1 resection was achieved in 85.54%, the overall 5-year PFS was 93.75%. Our rate of permanent new neurological deficit was 4.82% and the overall complication rate of 14.46%. The grade of PTE was proportional to tumor volume, 20 ± 2.8, 30 ± 5.3, and 34 ± 4.3 cm3 for grades 1, 2, and 3, respectively, positive cleft sign on image subtraction was predictive of good intraoperative cleavage plane and low grade cleavage plane (P = 0.04), and was a protective factor for postoperative neurological deficit (P = 0.02). Positive cleft sign in T2-FLAIR digital subtraction image is an independent predictor of good intraoperative cleavage plane, PTE is an independent predictor of the bad cleavage plane. Negative cleft sign in the image subtraction and a bad intraoperative cleavage plane are predictors of postoperative neurological deficit. PMID:27580930

  10. Noninvasive vascular displacement estimation for relative elastic modulus reconstruction in transversal imaging planes.

    PubMed

    Hansen, Hendrik H G; Richards, Michael S; Doyley, Marvin M; de Korte, Chris L

    2013-03-11

    Atherosclerotic plaque rupture can initiate stroke or myocardial infarction. Lipid-rich plaques with thin fibrous caps have a higher risk to rupture than fibrotic plaques. Elastic moduli differ for lipid-rich and fibrous tissue and can be reconstructed using tissue displacements estimated from intravascular ultrasound radiofrequency (RF) data acquisitions. This study investigated if modulus reconstruction is possible for noninvasive RF acquisitions of vessels in transverse imaging planes using an iterative 2D cross-correlation based displacement estimation algorithm. Furthermore, since it is known that displacements can be improved by compounding of displacements estimated at various beam steering angles, we compared the performance of the modulus reconstruction with and without compounding. For the comparison, simulated and experimental RF data were generated of various vessel-mimicking phantoms. Reconstruction errors were less than 10%, which seems adequate for distinguishing lipid-rich from fibrous tissue. Compounding outperformed single-angle reconstruction: the interquartile range of the reconstructed moduli for the various homogeneous phantom layers was approximately two times smaller. Additionally, the estimated lateral displacements were a factor of 2-3 better matched to the displacements corresponding to the reconstructed modulus distribution. Thus, noninvasive elastic modulus reconstruction is possible for transverse vessel cross sections using this cross-correlation method and is more accurate with compounding.

  11. Dual-view plane illumination microscopy for rapid and spatially isotropic imaging

    PubMed Central

    Kumar, Abhishek; Wu, Yicong; Christensen, Ryan; Chandris, Panagiotis; Gandler, William; McCreedy, Evan; Bokinsky, Alexandra; Colón-Ramos, Daniel A; Bao, Zhirong; McAuliffe, Matthew; Rondeau, Gary; Shroff, Hari

    2015-01-01

    We describe the construction and use of a compact dual-view inverted selective plane illumination microscope (diSPIM) for time-lapse volumetric (4D) imaging of living samples at subcellular resolution. Our protocol enables a biologist with some prior microscopy experience to assemble a diSPIM from commercially available parts, to align optics and test system performance, to prepare samples, and to control hardware and data processing with our software. Unlike existing light sheet microscopy protocols, our method does not require the sample to be embedded in agarose; instead, samples are prepared conventionally on glass coverslips. Tissue culture cells and Caenorhabditis elegans embryos are used as examples in this protocol; successful implementation of the protocol results in isotropic resolution and acquisition speeds up to several volumes per s on these samples. Assembling and verifying diSPIM performance takes ~6 d, sample preparation and data acquisition take up to 5 d and postprocessing takes 3–8 h, depending on the size of the data. PMID:25299154

  12. A novel readout method for focal plane array imaging in the presence of large dark current

    NASA Astrophysics Data System (ADS)

    Qiu, Changqing

    1999-08-01

    This research was an investigation of a novel readout method for focal plane array (FPA) optical imaging, especially for very sensitive detectors with large dark current. The readout method is based on periodically blocking the optical input enabling the removal of the dark current integration from the output. The research demonstrated that it is feasible to modulate the optical input with the designed readout circuit and thus achieve longer signal integration time to enhance the signal-to- noise ratio. Study of a proposed circuit model showed that in theory the correlated readout method could increase the output voltage swing and reduce the noise level by attenuating low frequency noise, thereby effectively improving the FPA dynamic range. Circuits based on standard CMOS circuitry were designed, simulated by PSpice, fabricated using Orbit 2μm n-well technology, and tested with a PI-4000 system. In the circuit evaluation, the output noise due to the clock switching phenomena, the gate signal feedthrough and the charge relaxation, was considered to be the critical problem. The most promising design for minimizing this problem had a CMOS current steering circuit at the input of a high CMRR operational amplifier. Simulation and test results showed that a modified capacitive transimpedance amplifier (CTIA) could subtract dark current output and reduce the output signal due to any difference between the frequencies of the optical input modulation signal and the switch modulation signal. In conclusion, the correlated readout circuit was shown to be a promising approach for advancing FPA technology.

  13. 320 x 256 Complementary Barrier Infrared Detector Focal Plane Array for Long-Wave Infrared Imaging

    NASA Technical Reports Server (NTRS)

    Nguyen, Jean; Rafol, Sir B.; Soibel, Alexander; Khoskhlagh, Arezou; Ting, David Z.-Y.; Liu, John K.; Mumolo, Jason M.; Gunapala, Sarath D.

    2012-01-01

    A 320 x 256 Complementary Barrier Infrared (CBIRD) focal plane array for long-wavelength infrared (LWIR) imaging is reported. The arrays were grown by molecular beam expitaxy (MBE) with a 300 period 1.9 um thick absorber. The mean dark current density of 2.2 x 10-4 A/cm2 was measured at an operating bias of 128 mV with a long wavelength cutoff of 8.8 ?m observed at 50% of the peak. The maximum quantum efficiency was 54% measured at 5.6 ?m. Operating at T = 80K, the array yielded an 81% fill factor with 97% operability. Good imagery with a mean noise equivalent different temperature (NE?T) of 18.6 mK and a mean detectivity of D* = 1.3 x 1011 cm-Hz1/2/W was achieved. The substrate was thinned using mechanical lapping and neither an AR coating nor a passivation layer was applied. This article provides the details of the fabrication process for achieving low-dark current LWIR CBIRD arrays. Discussion for an effective hard mask for excellent pattern transfer is given and appropriate mounting techniques for good thermal contact during the dry etching process is described. The challenges and differences between etching large 200 ?m test diodes and small 28 ?m FPA pixels are given.

  14. Image plane digital holographic microscope for the inspection of ferroelectric single crystals

    NASA Astrophysics Data System (ADS)

    Psota, Pavel; Mokrý, Pavel; Lédl, Vít; Vojtíšek, Petr

    2016-12-01

    One of the most important characteristics in the research and application of ferroelectric materials is the appearance of the domain patterns, which take place in phenomena such as ferroelectric phase transitions or ferroelectric switching. The ability to visualize domains is the key factor that enables the progress in the research of these extremely interesting phenomena. However, the three-dimensional visualization of the ferroelectric domain patterns in the whole volume of the ferroelectric single crystal is not a straightforward task. We present the optical method, which allows the acquisition of quantitative and qualitative data substantial for the ferroelectric domain research. The principle of the method is based on image plane digital holographic microscopy (DHM). We used DHM setup outcomes from a Mach-Zehnder type of interferometer and phase-shifting digital holography. The studied specimen is a single crystal of barium titanate. It is demonstrated that the use of solid-state thin-film transparent electrodes of indium tin oxide greatly reduces the unwanted wavefront distortions, which are frequently produced in liquid electrodes. Using this approach, it is possible to greatly improve the DHM measurements in low applied electric fields. Thanks to the properties of the setup, real-time observation of domain walls growth or existing patterns of the ferroelectric crystal is possible.

  15. The nonuniformity measurement and image processing algorithm evaluation for uncooled microbolometer infrared focal plane arrays

    NASA Astrophysics Data System (ADS)

    Qian, Yunsheng; Chang, BenKang; Zhang, Junju; Xing, Suxia; Yu, Shuizhong; Yang, Ji

    2005-01-01

    The great achievements were achieved in the manufacturing of uncooled microbolometer infrared focal plane arrays(UFPA). By this technique infrared system can be made in the formation of small volume, light weight, low price and being portable. It promotes greatly the utilization of infrared system in many fields. The main disadvantage of UFPA is non-uniformity. Despite non-uniformity of UFPA has been greatly improved, non-uniformity still restricts the performance of uncooled infrared system. In this paper, the attention is focused on the technology and methods measuring the non-uniformity of UFPA. The system that can measure the non-uniformity of UFPA and evaluate the image processing algorithms is developed. The measurement system consists of blackbody, infrared optics, control units, processing circuit, high-speed A/D converter, computer and software. To obtain the output signals of UFPA, the drive circuit and control circuit of thermoelectric stabilizer(TEC) of UFPA are developed. In the drive circuit, the CPLD device is employed to insure a small size circuit. In the TEC circuit, a kind of highly integrated and cost-effective, high-effiency, switch-mode driver is used to insure temperature stability of 0.01°C. The system is used to measure non-uniformity of microbolometer detectors which are produced by ULIS company. It can also present the evaluation of algorithm. The results are given and analyzed.

  16. Ultracompact Blue Dwarf Galaxies: Hubble Space Telescope Imaging and Stellar Population Analysis

    NASA Astrophysics Data System (ADS)

    Corbin, Michael R.; Vacca, William D.; Cid Fernandes, Roberto; Hibbard, John E.; Somerville, Rachel S.; Windhorst, Rogier A.

    2006-11-01

    We present deep Hubble Space Telescope (HST) Advanced Camera for Surveys/High Resolution Channel U-, narrow-V-, and I-band images of nine ``ultracompact'' blue dwarf galaxies (UCBDs) selected from the Sloan Digital Sky Survey (SDSS). We define UCBDs as local (z<0.01) star-forming galaxies having angular diameters less than 6" and physical diameters <1 kpc. They are also among the most metal-poor galaxies known, including objects having 12+log(O/H)<7.65, and are found to reside within voids. Both the HST images and the objects' SDSS optical spectra reveal that they are composites of young (~1-10 Myr) populations that dominate their light and older (~10 Gyr) populations that dominate their stellar masses, which we estimate to be ~107-108 Msolar. An intermediate-age (~107-109 yr) population is also indicated in most objects. The objects do not appear to be as dynamically disturbed as the prototype UCBD, POX 186, but the structure of several of them suggests that their current star formation has been triggered by the collisions/mergers of smaller clumps of stars. In one case, HS 0822+3542, the images resolve what may be two small (~100 pc) components that have recently collided, supporting this interpretation. In six of the objects much of the star formation is concentrated in young massive clusters, contributing to their compactness in ground-based images. The evidence that the galaxies consist mainly of ~10 Gyr old stars establishes that they are not protogalaxies, forming their first generation of stars. Their low metallicities are more likely to be the result of the escape of supernova ejecta, rather than youth.

  17. Blue camera of the Keck cosmic web imager, fabrication and testing

    NASA Astrophysics Data System (ADS)

    Rockosi, Constance; Cowley, David; Cabak, Jerry; Hilyard, David; Pfister, Terry

    2016-08-01

    The Keck Cosmic Web Imager (KCWI) is a new facility instrument being developed for the W. M. Keck Observatory and funded for construction by the Telescope System Instrumentation Program (TSIP) of the National Science Foundation (NSF). KCWI is a bench-mounted spectrograph for the Keck II right Nasmyth focal station, providing integral field spectroscopy over a seeing-limited field up to 20" x 33" in extent. Selectable Volume Phase Holographic (VPH) gratings provide high efficiency and spectral resolution in the range of 1000 to 20000. The dual-beam design of KCWI passed a Preliminary Design Review in summer 2011. The detailed design of the KCWI blue channel (350 to 700 nm) is now nearly complete, with the red channel (530 to 1050 nm) planned for a phased implementation contingent upon additional funding. KCWI builds on the experience of the Caltech team in implementing the Cosmic Web Imager (CWI), in operation since 2009 at Palomar Observatory. KCWI adds considerable flexibility to the CWI design, and will take full advantage of the excellent seeing and dark sky above Mauna Kea with a selectable nod-and-shuffle observing mode. In this paper, models of the expected KCWI sensitivity and background subtraction capability are presented, along with a detailed description of the instrument design. The KCWI team is lead by Caltech (project management, design and implementation) in partnership with the University of California at Santa Cruz (camera optical and mechanical design) and the W. M. Keck Observatory (program oversight and observatory interfaces). The optical design of the blue camera for the Keck Cosmic Web Imager (KCWI) by Harland Epps of the University of California, Santa Cruz is a lens assembly consisting of eight spherical optical elements. Half the elements are calcium fluoride and all elements are air spaced. The design of the camera barrel is unique in that all the optics are secured in their respective cells with an RTV annulus without additional hardware

  18. Raman imaging for determining the sequence of blue pen ink crossings.

    PubMed

    Braz, André; López-López, María; García-Ruiz, Carmen

    2015-04-01

    This manuscript presents a preliminary investigation on the applicability of Raman imaging for non-destructive and rapid analysis of blue crossing ink lines. The MCR method was used to facilitate visualization of the distribution of inks of the same colour and the most predominant Raman signature at the crossing was used to interpret the order of application of inks. Different pen ink types, different times separating the application of the two ink lines and different paper substrates were used. From the 90 Raman images examined, the correct order of application was determined in more than 60% by direct observation. The remainder cases were not as clear due to the uneven distribution of inks and the empty spaces similar to a net-like pattern observed at the crossing. This pattern was possibly caused by physical impediments (the first ink applied acting as a physical barrier) or chemical impediments (the two inks did not stick). Such impediments were more strongly observed in the crossings involving the U. Eye pen ink, causing the complete skipping of this ink line. Moreover, most crossings showed some mixing between the two inks and it was more accentuated when the times separating the application of the inks were shorter, since the ink was fresher. The use of white or certificate papers did not seem to influence in the inks distribution nor determining the order of the inks. Although this study provided useful insights regarding crossing ink lines, future statistic studies may be helpful for more objective examinations.

  19. Visualization of peripheral vasodilative indices in human skin by use of red, green, blue images

    NASA Astrophysics Data System (ADS)

    Nishidate, Izumi; Tanaka, Noriyuki; Kawase, Tatsuya; Maeda, Takaaki; Yuasa, Tomonori; Aizu, Yoshihisa; Yuasa, Tetsuya; Niizeki, Kyuichi

    2013-06-01

    We propose a method to visualize the arterial inflow, the vascular resistance, and the venous capacitance in the skin tissue from red, green, blue (RGB) digital color images. The arterial inflow and the venous capacitance in the skin tissue are visualized based on an increase in the rate of change in the total blood concentration and the change of the total blood concentration during upper limb occlusion at a pressure of 50 mmHg. The resultant arterial inflow with the measured mean arterial pressure also provides an image of the vascular resistance in human skin. The arterial inflow, the vascular resistance, and the venous capacitance acquired by the method are well correlated with those obtained from the conventional strain-gauge plethysmograph. The correlation coefficients R between the estimated values by the method and the measurements by the SPG are calculated to be 0.83 (P<0.001) for the arterial inflow, 0.77 (P<0.01) for the vascular resistance, and 0.77 (P<0.01) for the venous capacitance. The arterial inflow and the venous capacitance in the skin tissue are significantly higher in active subjects compared with the sedentary subjects, whereas the vascular resistance was significantly lower in the active subjects compared with the sedentary subjects. The results of the present study indicate the possibility of using the proposed method for evaluating the peripheral vascular functions in human skin.

  20. Development of a Nile-blue based chemodosimeter for Hg2+ in aqueous solution and its application in biological imaging.

    PubMed

    Hu, Mingming; Yin, Jianhua; Li, Yahong; Zhao, Xiaofang

    2015-03-01

    A Nile blue-based chemodosimeter was newly synthesized. It can detect Hg(2+) in aqueous solution based on desulfurization reaction. Upon its addition into aqueous Hg(2+) ion solution, it exhibited a considerable blue-shift in its absorption and obvious fluorescence quenching. The detection mechanism was proved by mass spectrometry analysis and Gaussian calculations. Detection at an emission of 685 nm was extremely sensitive, with a detection limit of 2.5 × 10(-9) mol/L. The fluorescent images in living cells and zebrafish demonstrate its potential for studying the accumulation of mercury species in organism.

  1. Super-resolution reconstruction in frequency, image, and wavelet domains to reduce through-plane partial voluming in MRI

    SciTech Connect

    Gholipour, Ali Afacan, Onur; Scherrer, Benoit; Prabhu, Sanjay P.; Warfield, Simon K.; Aganj, Iman; Sahin, Mustafa

    2015-12-15

    Purpose: To compare and evaluate the use of super-resolution reconstruction (SRR), in frequency, image, and wavelet domains, to reduce through-plane partial voluming effects in magnetic resonance imaging. Methods: The reconstruction of an isotropic high-resolution image from multiple thick-slice scans has been investigated through techniques in frequency, image, and wavelet domains. Experiments were carried out with thick-slice T2-weighted fast spin echo sequence on the Academic College of Radiology MRI phantom, where the reconstructed images were compared to a reference high-resolution scan using peak signal-to-noise ratio (PSNR), structural similarity image metric (SSIM), mutual information (MI), and the mean absolute error (MAE) of image intensity profiles. The application of super-resolution reconstruction was then examined in retrospective processing of clinical neuroimages of ten pediatric patients with tuberous sclerosis complex (TSC) to reduce through-plane partial voluming for improved 3D delineation and visualization of thin radial bands of white matter abnormalities. Results: Quantitative evaluation results show improvements in all evaluation metrics through super-resolution reconstruction in the frequency, image, and wavelet domains, with the highest values obtained from SRR in the image domain. The metric values for image-domain SRR versus the original axial, coronal, and sagittal images were PSNR = 32.26 vs 32.22, 32.16, 30.65; SSIM = 0.931 vs 0.922, 0.924, 0.918; MI = 0.871 vs 0.842, 0.844, 0.831; and MAE = 5.38 vs 7.34, 7.06, 6.19. All similarity metrics showed high correlations with expert ranking of image resolution with MI showing the highest correlation at 0.943. Qualitative assessment of the neuroimages of ten TSC patients through in-plane and out-of-plane visualization of structures showed the extent of partial voluming effect in a real clinical scenario and its reduction using SRR. Blinded expert evaluation of image resolution in

  2. Galectin-3 Reflects Mitral Annular Plane Systolic Excursion Being Assessed by Cardiovascular Magnetic Resonance Imaging

    PubMed Central

    Kim, Seung-Hyun; Natale, Michele; Hoffmann, Julia; Reckord, Nadine; Hoffmann, Ursula; Budjan, Johannes; Henzler, Thomas; Papavassiliu, Theano; Borggrefe, Martin; Bertsch, Thomas; Akin, Ibrahim

    2016-01-01

    Background. This study investigates whether serum levels of galectin-3 may reflect impaired mitral annular plane systolic excursion (MAPSE) in patients undergoing cardiac magnetic resonance imaging (cMRI). Methods. Patients undergoing cMRI during routine clinical care were included prospectively within an all-comers design. Blood samples for biomarker measurements were collected within 24 hours following cMRI. Statistical analyses were performed in all patients and in three subgroups according to MAPSE (MAPSE I: ≥11 mm, MAPSE II: ≥8 mm–<11 mm, and MAPSE III: <8 mm). Patients with right ventricular dysfunction (<50%) were excluded. Results. 84 patients were included in the study. Median LVEF was 59% (IQR 51–64%). Galectin-3 correlated significantly with NT-proBNP (r = 0.42, p = 0.0001). Galectin-3 increased significantly according to the different stages of impaired MAPSE (p = 0.006) and was able to discriminate both patients with impaired MAPSE <11 mm (area under the curve (AUC) = 0.645, p = 0.024) and <8 mm (AUC = 0.733, p = 0.003). Combining galectin-3 with NT-proBNP improved discrimination of MAPSE <8 mm (AUC 0.803, p = 0.0001). In multivariable logistic regression models galectin-3 was still associated with impaired MAPSE (MAPSE < 11 mm: odds ratio (OR) = 3.53, p = 0.018; MAPSE < 8 mm: OR = 3.18, p = 0.06). Conclusions. Galectin-3 reflects MAPSE being assessed by cardiac MRI. PMID:28044067

  3. Dual-plane stereoscopic particle image velocimetry: system set-up and its application on a lobed jet mixing flow

    NASA Astrophysics Data System (ADS)

    Hu, H.; Saga, T.; Kobayashi, T.; Taniguchi, N.; Yasuki, M.

    The technical basis and system set-up of a dual-plane stereoscopic particle image velocimetry (PIV) system, which can obtain the flow velocity (all three components) fields at two spatially separated planes simultaneously, is summarized. The simultaneous measurements were achieved by using two sets of double-pulsed Nd:Yag lasers with additional optics to illuminate the objective fluid flow with two orthogonally linearly polarized laser sheets at two spatially separated planes, as proposed by Kaehler and Kompenhans in 1999. The light scattered by the tracer particles illuminated by laser sheets with orthogonal linear polarization were separated by using polarizing beam-splitter cubes, then recorded by high-resolution CCD cameras. A three-dimensional in-situ calibration procedure was used to determine the relationships between the 2-D image planes and three-dimensional object fields for both position mapping and velocity three-component reconstruction. Unlike conventional two-component PIV systems or single-plane stereoscopic PIV systems, which can only get one-component of vorticity vectors, the present dual-plane stereoscopic PIV system can provide all the three components of the vorticity vectors and various auto-correlation and cross-correlation coefficients of flow variables instantaneously and simultaneously. The present dual-plane stereoscopic PIV system was applied to measure an air jet mixing flow exhausted from a lobed nozzle. Various vortex structures in the lobed jet mixing flow were revealed quantitatively and instantaneously. In order to evaluate the measurement accuracy of the present dual-plane stereoscopic PIV system, the measurement results were compared with the simultaneous measurement results of a laser Doppler velocimetry (LDV) system. It was found that both the instantaneous data and ensemble-averaged values of the stereoscopic PIV measurement results and the LDV measurement results agree well. For the ensemble-averaged values of the out-of-plane

  4. Methylene blue microbubbles as a model dual-modality contrast agent for ultrasound and activatable photoacoustic imaging.

    PubMed

    Jeon, Mansik; Song, Wentao; Huynh, Elizabeth; Kim, Jungho; Kim, Jeesu; Helfield, Brandon L; Leung, Ben Y C; Goertz, David E; Zheng, Gang; Oh, Jungtaek; Lovell, Jonathan F; Kim, Chulhong

    2014-01-01

    Ultrasound and photoacoustic imaging are highly complementary modalities since both use ultrasonic detection for operation. Increasingly, photoacoustic and ultrasound have been integrated in terms of hardware instrumentation. To generate a broadly accessible dual-modality contrast agent, we generated microbubbles (a standard ultrasound contrast agent) in a solution of methylene blue (a standard photoacoustic dye). This MB2 solution was formed effectively and was optimized as a dual-modality contrast solution. As microbubble concentration increased (with methylene blue concentration constant), photoacoustic signal was attenuated in the MB2 solution. When methylene blue concentration increased (with microbubble concentration held constant), no ultrasonic interference was observed. Using an MB2 solution that strongly attenuated all photoacoustic signal, high powered ultrasound could be used to burst the microbubbles and dramatically enhance photoacoustic contrast (>800-fold increase), providing a new method for spatiotemporal control of photoacoustic signal generation.

  5. Methylene blue microbubbles as a model dual-modality contrast agent for ultrasound and activatable photoacoustic imaging

    NASA Astrophysics Data System (ADS)

    Jeon, Mansik; Song, Wentao; Huynh, Elizabeth; Kim, Jungho; Kim, Jeesu; Helfield, Brandon L.; Leung, Ben Y. C.; Goertz, David E.; Zheng, Gang; Oh, Jungtaek; Lovell, Jonathan F.; Kim, Chulhong

    2014-01-01

    Ultrasound and photoacoustic imaging are highly complementary modalities since both use ultrasonic detection for operation. Increasingly, photoacoustic and ultrasound have been integrated in terms of hardware instrumentation. To generate a broadly accessible dual-modality contrast agent, we generated microbubbles (a standard ultrasound contrast agent) in a solution of methylene blue (a standard photoacoustic dye). This MB2 solution was formed effectively and was optimized as a dual-modality contrast solution. As microbubble concentration increased (with methylene blue concentration constant), photoacoustic signal was attenuated in the MB2 solution. When methylene blue concentration increased (with microbubble concentration held constant), no ultrasonic interference was observed. Using an MB2 solution that strongly attenuated all photoacoustic signal, high powered ultrasound could be used to burst the microbubbles and dramatically enhance photoacoustic contrast (>800-fold increase), providing a new method for spatiotemporal control of photoacoustic signal generation.

  6. The Effect of Through-Plane Motion on Left Ventricular Rotation: A Study Using Slice Following Harmonic Phase Imaging

    PubMed Central

    Brotman, David; Zhang, Ziheng; Sampath, Smita

    2012-01-01

    Non-invasive quantification of regional left ventricular (LV) rotation may improve understanding of cardiac function. Current methods employed to quantify rotation typically acquire data on a set of prescribed short-axis slices, neglecting effects due to through-plane myocardial motion. We combine principles of slice-following tagged imaging with harmonic phase analysis methods to account for through-plane motion in regional rotation measurements. We compare rotation and torsion measurements obtained using our method to those obtained from imaging datasets acquired without slice-following. Our results in normal volunteers demonstrate differences in the general trends of average and regional rotation-time plots in mid-basal slices, and of the rotation versus circumferential strain loops. We observe substantial errors in measured peak average rotation of the order of 58% for basal slices (due to change in the pattern of the curve), −6.6% for mid-ventricular slices, and −8.5% for apical slices; and an average error in base-to-apex torsion of 19% when through-plane motion is not considered. This study concludes that due to an inherent base-to-apex gradient in rotation that exists in the LV, accounting for through-plane motion is critical to the accuracy of LV rotation quantification. PMID:22700308

  7. Effect of through-plane motion on left ventricular rotation: a study using slice-following harmonic phase imaging.

    PubMed

    Brotman, David; Zhang, Ziheng; Sampath, Smita

    2013-05-01

    Noninvasive quantification of regional left ventricular rotation may improve understanding of cardiac function. Current methods used to quantify rotation typically acquire data on a set of prescribed short-axis slices, neglecting effects due to through-plane myocardial motion. We combine principles of slice-following tagged imaging with harmonic phase analysis methods to account for through-plane motion in regional rotation measurements. We compare rotation and torsion measurements obtained using our method to those obtained from imaging datasets acquired without slice-following. Our results in normal volunteers demonstrate differences in the general trends of average and regional rotation-time plots in midbasal slices and the rotation versus circumferential strain loops. We observe substantial errors in measured peak average rotation of the order of 58% for basal slices (due to change in the pattern of the curve), -6.6% for midventricular slices, and -8.5% for apical slices; and an average error in base-to-apex torsion of 19% when through-plane motion is not considered. This study concludes that due to an inherent base-to-apex gradient in rotation that exists in the left ventricular, accounting for through-plane motion is critical to the accuracy of left ventricular rotation quantification.

  8. Luminous blue variables: An imaging perspective on their binarity and near environment

    NASA Astrophysics Data System (ADS)

    Martayan, Christophe; Lobel, Alex; Baade, Dietrich; Mehner, Andrea; Rivinius, Thomas; Boffin, Henri M. J.; Girard, Julien; Mawet, Dimitri; Montagnier, Guillaume; Blomme, Ronny; Kervella, Pierre; Sana, Hugues; Štefl, Stanislav; Zorec, Juan; Lacour, Sylvestre; Le Bouquin, Jean-Baptiste; Martins, Fabrice; Mérand, Antoine; Patru, Fabien; Selman, Fernando; Frémat, Yves

    2016-03-01

    Context. Luminous blue variables (LBVs) are rare massive stars with very high luminosity. They are characterized by strong photometric and spectroscopic variability related to transient eruptions. The mechanisms at the origin of these eruptions is not well known. In addition, their formation is still problematic and the presence of a companion could help to explain how they form. Aims: This article presents a study of seven LBVs (about 20% of the known Galactic population), some Wolf-Rayet stars, and massive binaries. We probe the environments that surround these massive stars with near-, mid-, and far-infrared images, investigating potential nebula/shells and the companion stars. Methods: To investigate large spatial scales, we used seeing-limited and near diffraction-limited adaptive optics images to obtain a differential diagnostic on the presence of circumstellar matter and to determine their extent. From those images, we also looked for the presence of binary companions on a wide orbit. Once a companion was detected, its gravitational binding to the central star was tested. Tests include the chance projection probability, the proper motion estimates with multi-epoch observations, flux ratio, and star separations. Results: We find that two out of seven of LBVs may have a wide orbit companion. Most of the LBVs display a large circumstellar envelope or several shells. In particular, HD 168625, known for its rings, possesses several shells with possibly a large cold shell at the edge of which the rings are formed. For the first time, we have directly imaged the companion of LBV stars. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere, Chile, under projects number 085.D-0625(C), 087.D-0426(C, D), and archival data 383.D-0323(A).The reduced NACO images are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc

  9. Fast, Accurate and Precise Mid-Sagittal Plane Location in 3D MR Images of the Brain

    NASA Astrophysics Data System (ADS)

    Bergo, Felipe P. G.; Falcão, Alexandre X.; Yasuda, Clarissa L.; Ruppert, Guilherme C. S.

    Extraction of the mid-sagittal plane (MSP) is a key step for brain image registration and asymmetry analysis. We present a fast MSP extraction method for 3D MR images, based on automatic segmentation of the brain and on heuristic maximization of the cerebro-spinal fluid within the MSP. The method is robust to severe anatomical asymmetries between the hemispheres, caused by surgical procedures and lesions. The method is also accurate with respect to MSP delineations done by a specialist. The method was evaluated on 64 MR images (36 pathological, 20 healthy, 8 synthetic), and it found a precise and accurate approximation of the MSP in all of them with a mean time of 60.0 seconds per image, mean angular variation within a same image (precision) of 1.26o and mean angular difference from specialist delineations (accuracy) of 1.64o.

  10. Solid state active/passive night vision imager using continuous-wave laser diodes and silicon focal plane arrays

    NASA Astrophysics Data System (ADS)

    Vollmerhausen, Richard H.

    2013-04-01

    Passive imaging offers covertness and low power, while active imaging provides longer range target acquisition without the need for natural or external illumination. This paper describes a focal plane array (FPA) concept that has the low noise needed for state-of-the-art passive imaging and the high-speed gating needed for active imaging. The FPA is used with highly efficient but low-peak-power laser diodes to create a night vision imager that has the size, weight, and power attributes suitable for man-portable applications. Video output is provided in both the active and passive modes. In addition, the active mode is Class 1 eye safe and is not visible to the naked eye or to night vision goggles.

  11. An attempt to estimate out-of-plane lung nodule elongation in tomosynthesis images

    NASA Astrophysics Data System (ADS)

    Chodorowski, Artur; Arvidsson, Jonathan; Söderman, Christina; Svalkvist, Angelica; Johnsson, Šse A.; Bâth, Magnus

    2015-03-01

    In chest tomosynthesis (TS) the most commonly used reconstruction methods are based on Filtered Back Projection (FBP) algorithms. Due to the limited angular range of x-ray projections, FBP reconstructed data is typically associated with a low spatial resolution in the out-of-plane dimension. Lung nodule measures that depend on depth information such as 3D shape and volume are therefore difficult to estimate. In this paper the relation between features from FBP reconstructed lung nodules and the true out-of-plane nodule elongation is investigated and a method for estimating the out-of-plane nodule elongation is proposed. In order to study these relations a number of steps that include simulation of spheroidal-shaped nodules, insertion into synthetic data volumes, construction of TS-projections and FBP-reconstruction were performed. In addition, the same procedure was used to simulate nodules and insert them into clinical chest TS projection data. The reconstructed nodule data was then investigated with respect to in-plane diameter, out-of-plane elongation, and attenuation coefficient. It was found that the voxel value in each nodule increased linearly with nodule elongation, for nodules with a constant attenuation coefficient. Similarly, the voxel value increased linearly with in-plane diameter. These observations indicate the possibility to predict the nodule elongation from the reconstructed voxel intensity values. Such a method would represent a quantitative approach to chest tomosynthesis that may be useful in future work on volume and growth rate estimation of lung nodules.

  12. Performance of an Achromatic Focal Plane Mask for Exoplanet Imaging Coronagraphy

    NASA Technical Reports Server (NTRS)

    Newman, Kevin; Belikov, Ruslan; Pluzhnik, Eugene; Balasubramanian, Kunjithapatham; Wilson, Dan

    2014-01-01

    Coronagraph technology combined with wavefront control is close to achieving the contrast and inner working angle requirements in the lab necessary to observe the faint signal of an Earth-like exoplanet in monochromatic light. An important remaining technological challenge is to achieve high contrast in broadband light. Coronagraph bandwidth is largely limited by chromaticity of the focal plane mask, which is responsible for blocking the stellar PSF. The size of a stellar PSF scales linearly with wavelength; ideally, the size of the focal plane mask would also scale with wavelength. A conventional hard-edge focal plane mask has a fixed size, normally sized for the longest wavelength in the observational band to avoid starlight leakage. The conventional mask is oversized for shorter wavelengths and blocks useful discovery space. Recently we presented a solution to the size chromaticity challenge with a focal plane mask designed to scale its effective size with wavelength. In this paper, we analyze performance of the achromatic size-scaling focal plane mask within a Phase Induced Amplitude Apodization (PIAA) coronagraph. We present results from wavefront control around the achromatic focal plane mask, and demonstrate the size-scaling effect of the mask with wavelength. The edge of the dark zone, and therefore the inner working angle of the coronagraph, scale with wavelength. The achromatic mask enables operation in a wider band of wavelengths compared with a conventional hard-edge occulter.

  13. The WIYN one degree imager 2014: performance of the partially populated focal plane and instrument upgrade path

    NASA Astrophysics Data System (ADS)

    Harbeck, Daniel R.; Boroson, Todd; Lesser, Michael; Rajagopal, Jayadev; Yeatts, Andrey; Corson, Charles; Liu, Wilson; Dell'Antonio, Ian; Kotulla, Ralf; Ouellette, David; Hooper, Eric; Smith, Mike; Bredthauer, Richard; Martin, Pierre; Muller, Gary; Knezek, Patricia; Hunten, Mark

    2014-07-01

    The One Degree Imager (ODI) was deployed during the summer of 2012 at the WIYN 3.5m telescope, located on Kitt Peak near Tucson, AZ (USA). ODI is an optical imager designed to deliver atmosphere-limited image quality (≤ 0.4" FWHM) over a one degree field of view, and uses Orthogonal Transfer Array (OTA) detectors to also allow for on-chip tip/tilt image motion compensation. At this time, the focal plane is partially populated ("pODI") with 13 out of 64 OTA detectors, providing a central scientifically usable field of view of about 24' x 24'; four of the thirteen detectors are installed at outlying positions to probe image quality at all field angles. The image quality has been verified to be indeed better than 0.4'' FWHM over the full field when atmospheric conditions allow. Based on over one year of operations, we summarize pODIs performance and lessons learned. As pODI has proven the viability of the ODI instrument, the WIYN consortium is engaging in an upgrade project to add 12 more detectors to the focal plane enlarging the scientifically usable field of view to about 40' x 40'. A design change in the new detectors has successfully addressed a low light level charge transfer inefficiency.

  14. Visualization of Water Behavior in the In-plane and Throughplane Directions in a PEFC using a Neutron Image Intensifier

    NASA Astrophysics Data System (ADS)

    Murakawa, H.; Sugimoto, K.; Miyata, K.; Asano, H.; Takenaka, N.; Saito, Y.

    Water distributions of a polymer electrolyte fuel cell (PEFC) with 9-parallel channels during operation were visualized using a neutron radiography facility at B4 port in KUR (Kyoto University Research Reactor). An imaging system with a neutron image intensifier (I.I.) was employed for reducing the exposure time, and the water distributions in the in-plane and through-plane directions in the PEFC were alternately obtained every 20 sec. The accumulation processes from the GDL to the channels were confirmed. Water accumulated in the GDL at the cathode and evacuation into the channels started around 5 min. Water tended to accumulate at the edge of the ribs, and accumulated as water drops in the channels. The size of the water drops grew up to 1 mm which was the same size as the channel width and height, and the cell voltage was decreased because the liquid drops disturbed the air supply.

  15. Magnetic Prussian blue nanoparticles for targeted photothermal therapy under magnetic resonance imaging guidance.

    PubMed

    Fu, Guanglei; Liu, Wei; Li, Yanyan; Jin, Yushen; Jiang, Lingdong; Liang, Xiaolong; Feng, Shanshan; Dai, Zhifei

    2014-09-17

    This paper reported a core-shell nanotheranostic agent by growing Prussian blue (PB) nanoshells of 3-6 nm around superparamagnetic Fe3O4 nanocores for targeted photothermal therapy of cancer under magnetic resonance imaging (MRI) guidance. Both in vitro and in vivo experiments proved that the Fe3O4@PB core-shell nanoparticles showed significant contrast enhancement for T2-weighted MRI with the relaxivity value of 58.9 mM(-1)·s(-1). Simultaneously, the composite nanoparticles exhibited a high photothermal effect under irradiation of a near-infrared laser due to the strong absorption of PB nanoshells, which led to more than 80% death of HeLa cells with only 0.016 mg·mL(-1) of the nanoparticles with the aid of the magnetic targeting effect. Using tumor-bearing nude mice as the model, the near-infrared laser light ablated the tumor effectively in the presence of the Fe3O4@PB nanoparticles and the tumor growth inhibition was evaluated to be 87.2%. Capabilities of MRI, magnetic targeting, and photothermal therapy were thus integrated into a single agent to allow efficient MRI-guided targeted photothermal therapy. Most importantly, both PB and Fe3O4 nanoparticles were already clinically approved drugs, so the Fe3O4@PB nanoparticles as a theranostic nanomedicine would be particularly promising for clinical applications in the human body due to the reliable biosafety.

  16. Preoperative parathyroid localization by superimposed iodine-131 toluidine blue and technetium-99m pertechnetate imaging

    SciTech Connect

    Zwas, S.T.; Czerniak, A.; Boruchowsky, S.; Avigad, I.; Wolfstein, I.

    1987-03-01

    A new parathyroid scintigraphic localization study by a dual radioisotope technique using radioiodinated toluidine blue (RTB) for the parathyroids and /sup 99m/Tc for thyroid imaging is presented. A simple RTB labeling procedure achieving 99% tagging of the /sup 131/I-TB was used. The RTB was found to be a highly specific parathyroid radiotracer, consequently enabling superimposition of the delineated thyroid gland over the RTB avid parathyroid foci without a need for subtraction of the thyroid or vascular background. Forty-six patients with primary hyperparathyroidism underwent scintigraphic study prior to cervical (41 patients) or mediastinal (5 patients) exploration and 67 pathological parathyroid glands (34 adenomas and 33 hyperplasias) were excised. On follow-up, serum calcium level returned to normal in all patients. Correlation of the scintigraphic results with the surgical findings disclosed a sensitivity of 93%, with a specificity of 80% and an overall accuracy of 87%. This new simplified and specific RTB scintigraphic method justifies its use as a routine procedure for preoperative parathyroid scintigraphic localization in primary hyperparathyroidism.

  17. Vitamin B1 derived blue and green fluorescent carbon nanoparticles for cell-imaging application.

    PubMed

    Bhunia, Susanta Kumar; Pradhan, Nibedita; Jana, Nikhil R

    2014-05-28

    A carbon-based fluorescent nanoparticle is considered to be a new generation nontoxic nanoprobe suitable for various bioimaging and sensing applications. However, the synthesis of such a high-quality nanoparticle is challenging, and its application potential is mostly unexplored. Here we report a vitamin B1 carbonization-based approach for blue and green fluorescent carbon nanoparticles of <10 nm size with a fluorescence quantum of up to 76%. We found that carbonization of vitamin B1 in the presence of phosphate salt at ∼90-130 °C for about 2 h produces highly fluorescent carbon nanoparticles of 1-6 nm size. The particle size and fluorescence property can be controlled by varying the reaction temperature and nature of phosphate salt. Elemental analysis shows the incorporation of a large percentage (up to 48 wt %) of other elements (such as nitrogen, oxygen, phophorus, and sulfur) in the carbon matrix. The chemical structure of vitamin B1 (thiamine) is unique in a sense that it consists of a large number of heteroatoms along with unsaturated bonds and offers low-temperature carbonization with the formation of a nanoparticle having an optimum ratio of sp(2) and sp(3) carbon atoms. These carbon nanoparticles have high colloidal stability and stable fluorescence and have been used as fluorescent imaging probes.

  18. Red-green-blue fluorescent hollow carbon nanoparticles isolated from chromatographic fractions for cellular imaging.

    PubMed

    Gong, Xiaojuan; Hu, Qin; Paau, Man Chin; Zhang, Yan; Shuang, Shaomin; Dong, Chuan; Choi, Martin M F

    2014-07-21

    An as-synthesised hollow carbon nanoparticle (HC-NP) sample has been proved to be a relatively complex mixture, and its complexity can be reduced significantly by high-performance liquid chromatography. An unprecedented reduction in such complexity can reveal fractions of HC-NP with unique luminescence properties. While the UV-vis absorption profile for the HC-NP mixture is featureless, the HC-NP fractions do possess unique absorption bands and specific emission wavelengths. The HC-NP fractions are fully anatomised by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, displaying their fragmentation mass ion features. The shell thickness and crystal lattices of the selected HC-NP fractions are determined as 6.13, 8.31, 2.22, and 8.66 nm, and 0.37, 0.35, 0.33, and 0.32 nm by transmission electron microscopy, respectively. The fractionated HC-NP show profound differences in emission quantum yield, allowing for brighter HC-NP to be isolated from an apparent low quantum yield mixture. Finally, red, green and blue emissive HC-NP are isolated from the as-synthesised HC-NP sample. They show good photostability and have been demonstrated to be excellent probes for cellular imaging.

  19. Real-time 3D millimeter wave imaging based FMCW using GGD focal plane array as detectors

    NASA Astrophysics Data System (ADS)

    Levanon, Assaf; Rozban, Daniel; Kopeika, Natan S.; Yitzhaky, Yitzhak; Abramovich, Amir

    2014-03-01

    Millimeter wave (MMW) imaging systems are required for applications in medicine, communications, homeland security, and space technology. This is because there is no known ionization hazard for biological tissue, and atmospheric attenuation in this range of the spectrum is relatively low. The lack of inexpensive room temperature imaging systems makes it difficult to give a suitable MMW system for many of the above applications. 3D MMW imaging system based on chirp radar was studied previously using a scanning imaging system of a single detector. The system presented here proposes to employ a chirp radar method with a Glow Discharge Detector (GDD) Focal Plane Array (FPA) of plasma based detectors. Each point on the object corresponds to a point in the image and includes the distance information. This will enable 3D MMW imaging. The radar system requires that the millimeter wave detector (GDD) will be able to operate as a heterodyne detector. Since the source of radiation is a frequency modulated continuous wave (FMCW), the detected signal as a result of heterodyne detection gives the object's depth information according to value of difference frequency, in addition to the reflectance of the image. In this work we experimentally demonstrate the feasibility of implementing an imaging system based on radar principles and FPA of GDD devices. This imaging system is shown to be capable of imaging objects from distances of at least 10 meters.

  20. Transient state imaging of live cells using single plane illumination and arbitrary duty cycle excitation pulse trains.

    PubMed

    Mücksch, Jonas; Spielmann, Thiemo; Sisamakis, Evangelos; Widengren, Jerker

    2015-05-01

    We demonstrate the applicability of Single Plane Illumination Microscopy to Transient State Imaging (TRAST), offering sensitive microenvironmental information together with optical sectioning and reduced overall excitation light exposure of the specimen. The concept is verified by showing that transition rates can be determined accurately for free dye in solution and that fluorophore transition rates can be resolved pixel-wise in live cells. Furthermore, we derive a new theoretical framework for analyzing TRAST data acquired with arbitrary duty cycle pulse trains. By this analysis it is possible to reduce the overall measurement time and thereby enhance the frame rates in TRAST imaging.

  1. Simultaneous in-plane and out-of-plane displacement measurement based on a dual-camera imaging system and its application to inspection of large-scale space structures

    NASA Astrophysics Data System (ADS)

    Ri, Shien; Tsuda, Hiroshi; Yoshida, Takeshi; Umebayashi, Takashi; Sato, Akiyoshi; Sato, Eiichi

    2015-07-01

    Optical methods providing full-field deformation data have potentially enormous interest for mechanical engineers. In this study, an in-plane and out-of-plane displacement measurement method based on a dual-camera imaging system is proposed. The in-plane and out-of-plane displacements are determined simultaneously using two measured in-plane displacement data observed from two digital cameras at different view angles. The fundamental measurement principle and experimental results of accuracy confirmation are presented. In addition, we applied this method to the displacement measurement in a static loading and bending test of a solid rocket motor case (CFRP material; 2.2 m diameter and 2.3 m long) for an up-to-date Epsilon rocket developed by JAXA. The effectiveness and measurement accuracy is confirmed by comparing with conventional displacement sensor. This method could be useful to diagnose the reliability of large-scale space structures in the rocket development.

  2. Multispectral demosaicking considering out-of-focus problem for red-green-blue-near-infrared image sensors

    NASA Astrophysics Data System (ADS)

    Kwon, Ji Yong; Kang, Moon Gi

    2016-03-01

    A near-infrared (NIR) band provides information invisible to human eyes for discriminating and recognizing objects more clearly under low lighting conditions. To capture color and NIR images simultaneously, a multispectral filter array (MSFA) sensor is used. However, because lenses have different refractive indices for different wavelengths, lenses may fail to focus all rays to the same convergence. This is the reason an out-of-focus problem occurs and images are blurred. In this paper, a demosaicking algorithm that considers the out-of-focus problem is proposed. This algorithm is used by the MSFA of a red-green-blue-NIR image sensor to obtain color and NIR images. After the energies of the multispectral (MS) channels in the MSFA image are balanced to minimize aliasing, that image is filtered by the estimated low-pass kernel to generate a panchromatic (PAN) image. When an image is acquired, the out-of-focus problem and the formation process of the PAN image are modeled. The desired MS image is estimated by solving the least squares approach of the difference between the PAN and MS images based on the models. The experimental results show that the proposed algorithm performs well in estimating high-quality MS images and reduces the out-of-focus problem.

  3. jPOR: An ImageJ macro to quantify total optical porosity from blue-stained thin sections

    NASA Astrophysics Data System (ADS)

    Grove, Clayton; Jerram, Dougal A.

    2011-11-01

    A fast and effective method has been developed to measure total optical porosity (TOP) of blue resin-impregnated thin sections. This utilises a macro file (jPOR.txt) for ImageJ, which can be used on digital photomicrographs of thin sections. The method requires no specialised scientific equipment and can be run entirely using free to download software. Digital images are acquired from blue resin-impregnated thin sections using a conventional film scanner in the present study, though the technique can be applied to any high resolution colour digital acquired by different means (e.g., flat bed scanning, digital capture). Images are preprocessed using a newly developed custom 8-bit palette and analysed for porosity in ImageJ using the simple to use jPOR macro. Our method rapidly calculates TOP for batches of images with or without the option of user adjustment. Results are compared with conventional methods (e.g., to point counting), and tested with several users to estimate any user variability. jPOR provided comparable results to more time-consuming point counting, but with significantly less "counting error" and less interoperator variability than published point counting studies. The jPOR macro has been integrated into a macro tool set that can be configured to be run on ImageJ start up.

  4. Terahertz Wide-Angle Imaging and Analysis on Plane-wave Criteria Based on Inverse Synthetic Aperture Techniques

    NASA Astrophysics Data System (ADS)

    Gao, Jing Kun; Qin, Yu Liang; Deng, Bin; Wang, Hong Qiang; Li, Jin; Li, Xiang

    2016-04-01

    This paper presents two parts of work around terahertz imaging applications. The first part aims at solving the problems occurred with the increasing of the rotation angle. To compensate for the nonlinearity of terahertz radar systems, a calibration signal acquired from a bright target is always used. Generally, this compensation inserts an extra linear phase term in the intermediate frequency (IF) echo signal which is not expected in large-rotation angle imaging applications. We carried out a detailed theoretical analysis on this problem, and a minimum entropy criterion was employed to estimate and compensate for the linear-phase errors. In the second part, the effects of spherical wave on terahertz inverse synthetic aperture imaging are analyzed. Analytic criteria of plane-wave approximation were derived in the cases of different rotation angles. Experimental results of corner reflectors and an aircraft model based on a 330-GHz linear frequency-modulated continuous wave (LFMCW) radar system validated the necessity and effectiveness of the proposed compensation. By comparing the experimental images obtained under plane-wave assumption and spherical-wave correction, it also showed to be highly consistent with the analytic criteria we derived.

  5. A new inversion method of plasma density distribution of plasmasphere in the geomagnetic equatorial plane from IMAGE data

    NASA Astrophysics Data System (ADS)

    Li, Liang; Chen, Zhiqiang; Xu, Ronglan; Huang, Ya

    2011-12-01

    The plasma density distribution of plasmasphere in the geomagnetic equatorial plane can help us study the magnetosphere like plasmasphere, ionosphere and their kinetics. In this paper, we introduce a new inversion method, GE-ART, to calculate the plasma density distribution in the geomagnetic equatorial plane from the Extreme Ultraviolet (EUV) data of IMAGE satellite under the assumption that the plasma density is constant along each geomagnetic field line. The new GE-ART algorithm was derived from the traditional Algebraic Reconstruction Techniques (ART) in Computed Tomography (CT) which was different from the several existing methods. In this new method, each value of the EUV image data was back-projected evenly to the geomagnetic field lines intersected by this EUV sight. A 3-D inversion matrix was produced by the contributions of all the voxels contained in the plasmasphere covered by the EUV sensor. That is, we considered that each value of the EUV image data was relative to the plasma densities of all the voxels passed through by the corresponding EUV radiation, which is the biggest difference to all the existing inversion methods. Finally, the GE-ART algorithm was evaluated by the real EUV data from the IMAGE satellite.

  6. 1024 x 1024 pixel mid-wavelength and long-wavelength infrared QWIP focal plane arrays for imaging applications

    NASA Technical Reports Server (NTRS)

    Gunapala, S. D.; Bandara, S. V.; Liu, J. K.; Hill, C. J.; Rafol, S. B.; Mumolo, J. M.; Trinh, J. T.; Tidrow, M. Z.; LeVan, P. D.

    2005-01-01

    Mid-wavelength infrared (MWIR) and long-wavelength infrared (LWIR) 10(24) x 10(24) pixel quantum well infrared photodetector (QWIP) focal planes have been demonstrated with excellent imaging performance. The MWIR QWIP detector array has demonstrated a noise equivalent differential temperature (NEAT) of 17 mK at a 95 K operating temperature with f/2.5 optics at 300 K background and the LWIR detector array has demonstrated a NEAT of 13 mK at a 70 K operating temperature with the same optical and background conditions as the MWIR detector array after the subtraction of system noise. Both MWIR and LWIR focal planes have shown background limited performance (BLIP) at 90 K and 70 K operating temperatures respectively, with similar optical and background conditions. In this paper, we will discuss the performance in terms of quantum efficiency, NE(delta)T, uniformity, operability and modulation transfer functions.

  7. Real Time Imaging Analysis Using a Terahertz Quantum Cascade Laser and a Microbolometer Focal Plane Array

    DTIC Science & Technology

    2008-12-01

    sensing area, assuming that the device remains approximately in thermodynamic equilibrium, the heat balance equation can be expressed as o d TP G T C...11. Modulated radiation incident on focal plane array. .........................................17 Figure 12. Temperature change in a pixel for P0...for b) 4.3 and c) 4.9 THz windows. HITRAN (solid line) and FTIR results (dashed line). Lasing spectra of QCL’s are depicted with dashed vertical

  8. Benchmarking of depth of field for large out-of-plane deformations with single camera digital image correlation

    NASA Astrophysics Data System (ADS)

    Van Mieghem, Bart; Ivens, Jan; Van Bael, Albert

    2017-04-01

    A problem that arises when performing stereo digital image correlation in applications with large out-of-plane displacements is that the images may become unfocused. This unfocusing could result in correlation instabilities or inaccuracies. When performing DIC measurements and expecting large out-of-plane displacements researchers either trust on their experience or use the equations from photography to estimate the parameters affecting the depth of field (DOF) of the camera. A limitation of the latter approach is that the definition of sharpness is a human defined parameter and that it does not reflect the performance of the digital image correlation system. To get a more representative DOF value for DIC applications, a standardised testing method is presented here, making use of real camera and lens combinations as well as actual image correlation results. The method is based on experimental single camera DIC measurements of a backwards moving target. Correlation results from focused and unfocused images are compared and a threshold value defines whether or not the correlation results are acceptable even if the images are (slightly) unfocused. By following the proposed approach, the complete DOF of a specific camera/lens combination as function of the aperture setting and distance from the camera to the target can be defined. The comparison between the theoretical and the experimental DOF results shows that the achievable DOF for DIC applications is larger than what theoretical calculations predict. Practically this means that the cameras can be positioned closer to the target than what is expected from the theoretical approach. This leads to a gain in resolution and measurement accuracy.

  9. Photometric-based recovery of illuminant-free color images using a red-green-blue digital camera

    NASA Astrophysics Data System (ADS)

    Luis Nieves, Juan; Plata, Clara; Valero, Eva M.; Romero, Javier

    2012-01-01

    Albedo estimation has traditionally been used to make computational simulations of real objects under different conditions, but as yet no device is capable of measuring albedo directly. The aim of this work is to introduce a photometric-based color imaging framework that can estimate albedo and can reproduce the appearance both indoors and outdoors of images under different lights and illumination geometry. Using a calibration sample set composed of chips made of the same material but different colors and textures, we compare two photometric-stereo techniques, one of them avoiding the effect of shadows and highlights in the image and the other ignoring this constraint. We combined a photometric-stereo technique and a color-estimation algorithm that directly relates the camera sensor outputs with the albedo values. The proposed method can produce illuminant-free images with good color accuracy when a three-channel red-green-blue (RGB) digital camera is used, even outdoors under solar illumination.

  10. In-plane rigid-body vibration mode characterization with a nanometer resolution by stroboscopic imaging of a microstructured pattern.

    PubMed

    Sandoz, Patrick; Friedt, Jean-Michel; Carry, Emile

    2007-02-01

    This article introduces an improved approach for the characterization of in-plane rigid-body vibration, based on digital processing of stroboscopic images of the moving part. The method involves a sample preparation step, in order to pattern a periodic microstructure on the vibrating device, for instance, by focused ion beam milling. An image processing method has then been developed to perform the optimum reconstruction of this a priori known object feature. In-plane displacement and rotation are deduced simultaneously with a high resolution (10-2 pixel and 0.5 x 10(-3) rad, respectively). The measurement principle combines phase measurements-that provide the high resolution-with correlation-that unwraps the phase with the proper phase constants. The vibration modes of a tuning fork are used for demonstrating the capabilities of the method. For applications allowing the sample preparation, the proposed methodology is more convenient than common interference methods or image processing techniques for the characterization of the vibration modes, even for amplitudes in the nanometer range.

  11. Transport of Intensity phase imaging by intensity spectrum fitting of exponentially spaced defocus planes.

    PubMed

    Jingshan, Zhong; Claus, Rene A; Dauwels, Justin; Tian, Lei; Waller, Laura

    2014-05-05

    We propose an alternative method for solving the Transport of Intensity equation (TIE) from a stack of through-focus intensity images taken by a microscope or lensless imager. Our method enables quantitative phase and amplitude imaging with improved accuracy and reduced data capture, while also being computationally efficient and robust to noise. We use prior knowledge of how intensity varies with propagation in the spatial frequency domain in order to constrain a fitting algorithm [Gaussian process (GP) regression] for estimating the axial intensity derivative. Solving the problem in the frequency domain inspires an efficient measurement scheme which captures images at exponentially spaced focal steps, significantly reducing the number of images required. Low-frequency artifacts that plague traditional TIE methods can be suppressed without an excessive number of captured images. We validate our technique experimentally by recovering the phase of human cheek cells in a brightfield microscope.

  12. An abuttable CCD imager for visible and X-ray focal plane arrays

    NASA Technical Reports Server (NTRS)

    Burke, Barry E.; Mountain, Robert W.; Harrison, David C.; Bautz, Marshall W.; Doty, John P.

    1991-01-01

    A frame-transfer silicon charge-coupled-device (CCD) imager has been developed that can be closely abutted to other imagers on three sides of the imaging array. It is intended for use in multichip arrays. The device has 420 x 420 pixels in the imaging and frame-store regions and is constructed using a three-phase triple-polysilicon process. Particular emphasis has been placed on achieving low-noise charge detection for low-light-level imaging in the visible and maximum energy resolution for X-ray spectroscopic applications. Noise levels of 6 electrons at 1-MHz and less than 3 electrons at 100-kHz data rates have been achieved. Imagers have been fabricated on 1000-Ohm-cm material to maximize quantum efficiency and minimize split events in the soft X-ray regime.

  13. Feasibility of Multi-Plane-Transmit Beamforming for Real-time Volumetric Cardiac Imaging: A Simulation Study.

    PubMed

    Chen, Yinran; Tong, Ling; Ortega, Alejandra; Luo, Jianwen; D'hooge, Jan

    2017-01-10

    Today's three-dimensional (3-D) cardiac ultrasound imaging systems suffer from relatively low spatial and temporal resolution, limiting their applicability in daily clinical practice. To address this problem, 3-D diverging wave imaging with spatial coherent compounding (DWC) as well as 3-D multi-line-transmit (MLT) imaging have recently been proposed. Currently, the former improves the temporal resolution significantly at the expense of image quality and the risk of introducing motion artifacts whereas the latter only provides a moderate gain in volume rate but mostly preserves quality. In this study, a new technique for real-time volumetric cardiac imaging is proposed by combining the strengths of both approaches. Hereto, multiple planar (i.e., 2-D) diverging waves are simultaneously transmitted in order to scan the 3-D volume, i.e., multi-plane-transmit (MPT) beamforming. The performance of a 3MPT imaging system was contrasted to that of a 3-D DWC system and that of a 3-D MLT system by computer simulations during both static and moving conditions of the target structures while operating at similar volume rate. It was demonstrated that for stationary targets, the 3MPT imaging system was competitive with both the 3-D DWC and 3-D MLT systems in terms of spatial resolution and side lobe levels (i.e., image quality). However, for moving targets, the image quality quickly deteriorated for the 3-D DWC systems while it remained stable for the 3MPT system while operating at twice the volume rate of the 3D-MLT system. The proposed MPT beamforming approach was thus demonstrated to be feasible and competitive to state-of-the-art methodologies.

  14. Imaging the in-plane distribution of helium precipitates at a Cu/V interface

    DOE PAGES

    Chen, Di; Li, Nan; Yuryev, Dina; ...

    2017-02-15

    Here, we describe a transmission electron microscopy investigation of the distribution of helium precipitates within the plane of an interface between Cu and V. Statistical analysis of precipitate locations reveals a weak tendency for interfacial precipitates to align alongmore » $$\\langle$$110$$\\rangle$$-type crystallographic directions within the Cu layer. Comparison of these findings with helium-free Cu/V interfaces suggests that the precipitates may be aggregating preferentially along atomic-size steps in the interface created by threading dislocations in the Cu layer. Our observations also suggest that some precipitates may be aggregating along intersections between interfacial misfit dislocations.« less

  15. Infrared imaging of cotton fibers using a focal-plane array detector

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vibrational spectroscopy studies can be used to examine the quality and structure of cotton fibers. An emerging area of research relates to the imaging of cotton fibers. Herein, we report the use of a Fourier-transform infrared (FTIR) microscope to image developing cotton fibers. Studies were perfor...

  16. Inverse-synthetic-aperture imaging of trees over a ground plane

    SciTech Connect

    Zittel, D.H.; Brock, B.C.; Littlejohn, J.H.; Patitz, W.E.

    1995-11-01

    Recent data collections with the Sandia VHF-UHF synthetic-aperture radar have yielded surprising results; trees appear brighter in the images than expected! In an effort to understand this phenomenon, various small trees have been measured on the Sandia folded compact range with the inverse-synthetic-aperture imaging system. A compilation of these measurements is contained in this report.

  17. Snapshot linear-Stokes imaging spectropolarimeter using division-of-focal-plane polarimetry and integral field spectroscopy

    PubMed Central

    Mu, Tingkui; Pacheco, Shaun; Chen, Zeyu; Zhang, Chunmin; Liang, Rongguang

    2017-01-01

    In this paper, the design and experimental demonstration of a snapshot linear-Stokes imaging spectropolarimeter (SLSIS) is presented. The SLSIS, which is based on division-of-focal-plane polarimetry with four parallel linear polarization channels and integral field spectroscopy with numerous slit dispersive paths, has no moving parts and provides video-rate Stokes-vector hyperspectral datacubes. It does not need any scanning in the spectral, spatial or polarization dimension and offers significant advantages of rapid reconstruction without heavy computation during post-processing. The principle and the experimental setup of the SLSIS are described in detail. The image registration, Stokes spectral reconstruction and calibration procedures are included, and the system is validated using measurements of tungsten light and a static scene. The SLSIS’s snapshot ability to resolve polarization spectral signatures is demonstrated using measurements of a dynamic scene. PMID:28191819

  18. Snapshot linear-Stokes imaging spectropolarimeter using division-of-focal-plane polarimetry and integral field spectroscopy

    NASA Astrophysics Data System (ADS)

    Mu, Tingkui; Pacheco, Shaun; Chen, Zeyu; Zhang, Chunmin; Liang, Rongguang

    2017-02-01

    In this paper, the design and experimental demonstration of a snapshot linear-Stokes imaging spectropolarimeter (SLSIS) is presented. The SLSIS, which is based on division-of-focal-plane polarimetry with four parallel linear polarization channels and integral field spectroscopy with numerous slit dispersive paths, has no moving parts and provides video-rate Stokes-vector hyperspectral datacubes. It does not need any scanning in the spectral, spatial or polarization dimension and offers significant advantages of rapid reconstruction without heavy computation during post-processing. The principle and the experimental setup of the SLSIS are described in detail. The image registration, Stokes spectral reconstruction and calibration procedures are included, and the system is validated using measurements of tungsten light and a static scene. The SLSIS’s snapshot ability to resolve polarization spectral signatures is demonstrated using measurements of a dynamic scene.

  19. Real-time, continuous-wave terahertz imaging using a microbolometer focal-plane array

    NASA Technical Reports Server (NTRS)

    Hu, Qing (Inventor); Min Lee, Alan W. (Inventor)

    2010-01-01

    The present invention generally provides a terahertz (THz) imaging system that includes a source for generating radiation (e.g., a quantum cascade laser) having one or more frequencies in a range of about 0.1 THz to about 10 THz, and a two-dimensional detector array comprising a plurality of radiation detecting elements that are capable of detecting radiation in that frequency range. An optical system directs radiation from the source to an object to be imaged. The detector array detects at least a portion of the radiation transmitted through the object (or reflected by the object) so as to form a THz image of that object.

  20. Volume-scanning three-dimensional display that uses an inclined image plane.

    PubMed

    Miyazaki, D; Matsushita, K

    2001-07-10

    A novel three-dimensional display based on a volume-scanning method that uses an inclined light-source array and a mirror scanner is proposed. With this technique it is possible to display three-dimensional images that satisfy all factors for human stereoscopic vision. Three-dimensional images of 8 x 8 x 8 pixels, 40 mm x 40 mm x 40 mm in size, with a frame rate of 12.7 Hz were obtained as real images through an experimental system that uses a galvanometer mirror and a LED array.

  1. Correlation of right ventricular ejection fraction and tricuspid annular plane systolic excursion in tetralogy of Fallot by magnetic resonance imaging.

    PubMed

    Morcos, Peter; Vick, G Wesley; Sahn, David J; Jerosch-Herold, Michael; Shurman, Alan; Sheehan, Florence H

    2009-03-01

    The correlation between right ventricular ejection fraction (RVEF) and tricuspid annular plane systolic excursion (TAPSE) by two-dimensional (2-D) echo has been repeatedly validated, but not by magnetic resonance imaging (MRI) nor in patients with congenital heart disease. We tested whether TAPSE measurements by MRI correlate with RVEF in surgically repaired tetralogy of Fallot (TOF) patients. TAPSE was measured from systolic displacement of the RV-freewall/tricuspid annular plane junction in the apical 4-chamber view in 7 normal subjects and 14 TOF patients. The RV was reconstructed in 3-D from manually traced borders on MR images to compute true EF. Because we previously observed discrepancy between TAPSE and RVEF in the presence of regional dysfunction, we also analyzed RV wall motion in terms of regional stroke volume at 20 short axis slices from apex to tricuspid annulus. RVEF was 52 +/- 3% in normal subjects and 41 +/- 9% in TOF (P < 0.01). TAPSE correlated weakly (r = 0.50, P < 0.05) with RVEF. TOF patients exhibited increased regional stroke volume from apical portions of the RV and decreased regional stroke volume at the base compared to normal (P < 0.05 at 15 of 20 slices). Regional stroke volume in apical slices correlated inversely with RVEF such that patients with higher apical stroke volume had lower RVEF (P < 0.05). TAPSE is not a reliable measure of RVEF in TOF by MRI. TAPSE may be of limited use in conditions that exhibit abnormal regional contraction.

  2. HEB heterodyne focal plane arrays: a terahertz technology for high sensitivity near-range security imaging systems

    NASA Astrophysics Data System (ADS)

    Gerecht, Eyal; Gu, Dazhen; Yngvesson, Sigfrid; Rodriguez-Morales, Fernando; Zannoni, R.; Nicholson, John

    2005-05-01

    We have achieved the first demonstration of a low-noise heterodyne array operating at a frequency above 1 THz (1.6 THz). The prototype array has three elements, consisting of NbN hot electron bolometer (HEB) detectors on silicon substrates. We use a quasi-optical design to couple the signal and local oscillator (LO) power to the detector. We also demonstrate, for the first time, how the HEB detectors can be intimately integrated in the same block with monolithic microwave integrated circuit (MMIC) IF amplifiers. Such focal plane arrays can be increased in size to a few hundred elements using the next generation fabrication architecture for compact and easy assembly. Future HEB-based focal plane arrays will make low-noise heterodyne imaging systems with high angular resolution possible from 500 GHz to several terahertz. Large low-noise HEB arrays are well suited for real-time video imaging at any frequency over the entire terahertz spectrum. This is made possible by virtue of the extremely low local oscillator power requirements of the HEB detectors (a few hundred nanowatts to a microwatt per pixel). The operating temperature is 4 to 6 K, which can be provided by a compact and mobile cryocooler system, developed as a spin-off from the space program. The terahertz HEB imager consists of a computer-controlled optical system mounted on an elevation and azimuth scanning translator which provides a two-dimensional image of the target. We present preliminary measured data at the symposium for a terahertz security system of this type.

  3. Clockwork blue: on the evolution of non-image-forming retinal photoreceptors in marine and terrestrial vertebrates

    NASA Astrophysics Data System (ADS)

    Erren, T. C.; Erren, M.; Lerchl, A.; Meyer-Rochow, V. B.

    2008-04-01

    This paper presents a hypothesis that could explain why blue light appears to dominate non-image-forming (NIF) ocular photoreception in marine as well as terrestrial vertebrates. Indeed, there is more and more evidence suggesting that ‘novel’ retinal photoreceptors, which are sensitive to blue light and were only discovered in the 1990s, could be a feature shared by all vertebrates. In our view, blue light photoreception evolved and persisted as NIF photoreception because it has been useful in the colonisation of extensive photo-dependent oceanic habitats and facilitated the move of vertebrates from an aquatic to a terrestrial environment. Because the available scattered evidence is compatible with the validity of our hypothesis, we hope that our rationale will be followed up. Indeed, it (1) involves testable predictions, (2) provides plausible explanations for previous observations, (3) unites phenomena not previously considered related to one another and (4) suggests tests that have not been carried out before. Overall, our approach not only embraces cross-disciplinary links; it, moreover, serves as a reminder of an all-embracing evolutionary history, especially with regard to a ubiquitous photoreceptive ‘clockwork-blue’ in marine and terrestrial vertebrates.

  4. Radiation Channels Close to a Plasmonic Nanowire Visualized by Back Focal Plane Imaging

    PubMed Central

    Hartmann, Nicolai; Piatkowski, Dawid; Ciesielski, Richard; Mackowski, Sebastian; Hartschuh, Achim

    2014-01-01

    We investigated the angular radiation patterns, a key characteristic of an emitting system, from individual silver nanowires decorated with rare earth ion-doped nanocrystals. Back focal plane radiation patterns of the nanocrystal photoluminescence after local two-photon excitation can be described by two emission channels: Excitation of propagating surface plasmons in the nanowire followed by leakage radiation and direct dipolar emission observed also in the absence of the nanowire. Theoretical modeling reproduces the observed radiation patterns which strongly depend on the position of excitation along the nanowire. Our analysis allows to estimate the branching ratio into both emission channels and to determine the diameter dependent surface plasmon quasi-momentum, important parameters of emitter-plasmon structures. PMID:24131299

  5. A Comparison between Compounding Techniques using Large Beam-Steered Plane Wave Imaging for Blood Vector Velocity Imaging in a Carotid Artery Model.

    PubMed

    Saris, Anne; Hansen, Hendrik; Fekkes, Stein; Nillesen, Maartje; Rutten, Marcel; de Korte, Chris

    2016-09-07

    Conventional color Doppler imaging is limited, since it only provides velocity estimates along the ultrasound beam direction for a restricted field of view at a limited frame rate. High frame rate speckle tracking, using plane wave transmits, has shown potential for 2D blood velocity estimation. However, due to the lack of focusing in transmit, image quality gets reduced, which hampers speckle tracking. Although ultrafast imaging facilitates improved clutter filtering, it still remains a major challenge in blood velocity estimation. Signal drop-outs and poor velocity estimates are still present for high beam-to-flow angles and low blood flow velocities. In this work, ultrafast plane wave imaging was combined with multi-scale speckle tracking to assess the 2D blood velocity vector in a common carotid artery (CCA) flow field. A multi-angled plane wave imaging sequence was used to compare the performance of displacement compounding, coherent compounding and compound speckle tracking. Zero-degree plane wave imaging was also evaluated. The performance of the methods was evaluated before and after clutter filtering for the large range of velocities (0 to 1.5 m/s) that are normally present in a healthy CCA during the cardiac cycle. An extensive simulation study was performed, based on a sophisticated model of the CCA, to investigate and evaluate the performance of the methods at different pulse repetition frequencies and signal-to-noise levels. In vivo data were acquired of a healthy carotid artery bifurcation to support the simulation results. In general, methods utilizing compounding after speckle tracking, i.e., displacement compounding and compound speckle tracking, were least affected by clutter filtering and provided the most robust and accurate estimates for the entire velocity range. Displacement compounding, which uses solely axial information to estimate the velocity vector, provided most accurate velocity estimates, although it required sufficiently high pulse

  6. A Comparison Between Compounding Techniques Using Large Beam-Steered Plane Wave Imaging for Blood Vector Velocity Imaging in a Carotid Artery Model.

    PubMed

    Saris, Anne E C M; Hansen, Hendrik H G; Fekkes, Stein; Nillesen, Maartje M; Rutten, Marcel C M; de Korte, Chris L

    2016-11-01

    Conventional color Doppler imaging is limited, since it only provides velocity estimates along the ultrasound beam direction for a restricted field of view at a limited frame rate. High-frame-rate speckle tracking, using plane wave transmits, has shown potential for 2-D blood velocity estimation. However, due to the lack of focusing in transmit, image quality gets reduced, which hampers speckle tracking. Although ultrafast imaging facilitates improved clutter filtering, it still remains a major challenge in blood velocity estimation. Signal dropouts and poor velocity estimates are still present for high beam-to-flow angles and low blood flow velocities. In this paper, ultrafast plane wave imaging was combined with multiscale speckle tracking to assess the 2-D blood velocity vector in a common carotid artery (CCA) flow field. A multiangled plane wave imaging sequence was used to compare the performance of displacement compounding, coherent compounding, and compound speckle tracking. Zero-degree plane wave imaging was also evaluated. The performance of the methods was evaluated before and after clutter filtering for the large range of velocities (0-1.5 m/s) that are normally present in a healthy CCA during the cardiac cycle. An extensive simulation study was performed, based on a sophisticated model of the CCA, to investigate and evaluate the performance of the methods at different pulse repetition frequencies and signal-to-noise levels. In vivo data were acquired of a healthy carotid artery bifurcation to support the simulation results. In general, methods utilizing compounding after speckle tracking, i.e., displacement compounding and compound speckle tracking, were least affected by clutter filtering and provided the most robust and accurate estimates for the entire velocity range. Displacement compounding, which uses solely axial information to estimate the velocity vector, provided most accurate velocity estimates, although it required sufficiently high pulse

  7. The high resolution X-ray imaging detector planes for the MIRAX mission

    NASA Astrophysics Data System (ADS)

    Rodrigues, B. H. G.; Grindlay, J. E.; Allen, B.; Hong, J.; Barthelmy, S.; Braga, J.; D'Amico, F.; Rothschild, R. E.

    2013-09-01

    The MIRAX X-ray observatory, the first Brazilian-led astrophysics space mission, is designed to perform an unprecedented wide-field, wide-band hard X-ray (5-200 keV) survey of Galactic X-ray transient sources. In the current configuration, MIRAX will carry a set of four coded-masks telescopes with high spatial resolution Cadmium Zinc Telluride (CZT) detector planes, each one consisting of an array of 64 closely tiled CZT pixelated detectors. Taken together, the four telescopes will have a total detection area of 959 cm2, a large field of view (60° × 60° FWHM), high angular resolution for this energy range (6 arcmin) and very good spectral resolution ( ~ 2 keV @ 60 keV). A stratospheric balloon-borne prototype of one of the MIRAX telescopes has been developed, tested and flown by the Harvard-Smithsonian Center for Astrophysics (CfA) as part of the ProtoEXIST program. In this paper we show results of validation and calibration tests with individual CZT detectors of the ProtoEXIST second generation experiment (P2). Each one of 64 detector units of the P2 detector plane consists of an ASIC, developed by Caltech for the NuSTAR telescope, hybridized to a CZT crystal with 0.6 mm pixel size. The performance of each detector was evaluated using radioactive sources in the laboratory. The calibration results show that the P2 detectors have average energy resolution of ~ 2.1 keV @ 60 keV and 2.3 @ 122 keV. P2 was also successfully tested on near-space environment on a balloon flight, demonstrating the detector unit readiness for integration on a space mission telescope, as well as satisfying all MIRAX mission requirements.

  8. Large scale rock slope release planes imaged by differential ground based InSAR at Randa, Switzerland

    NASA Astrophysics Data System (ADS)

    Gischig, V.; Loew, S.; Kos, A.; Raetzo, H.

    2009-04-01

    In April and May of 1991 a steep rock slope above the village of Randa (Valais, Switzerland) failed in two events, releasing a total rock volume of 30 million m3. The rock mass behind the back scarp contains several million cubic meters of unstable gneisses and schists which are moving with a maximum rate of about 2 cm/yr. Different geodetic, geotechnical and geophysical techniques were applied to monitor this new instability and to determine its spatial extent. However, the boundaries of the instability could only be roughly estimated so far. For this reason five ground based differential InSAR surveys (GB-DInSAR) were carried out between 2005 and 2007 from the opposite valley flank at a distance to target of 1.3 to 1.9 km. These surveys provide displacements maps of four different time intervals with a spatial resolution of 2 to 6 m and an accuracy of less than 1 mm. These datasets reveal interesting new insights into the spatial distribution of displacements and significantly contribute to the kinematic interpretation of the ongoing movements. We found that the lower boundary of the instability is a narrow rupture plane which coincides with a primary lithological boundary on the slope. The intersection line between this basal rupture plane and the steep rock cliff extents over at least 200 m meters. It is possible to identify this structure on helicopter-based high resolution images and a LiDAR DTM of the failure surface. The eastern boundary of the instability also presents itself as a sharp line separating stable bedrock from a strongly fractured rock mass moving about 1 cm/yr along the line of sight. This lateral release plane is formed by a steeply east dipping tectonic fault plane, with subhorizontal striations and an exposed surface area of about 10'000 square meters. In the north-east of the instability the lateral boundaries crop out on surfaces that have an acute angle to the line of sight or lie in the shadow of the radar. Here the boundaries of the

  9. Left ventricular dyssynchrony assessed by two three-dimensional imaging modalities: phase analysis of gated myocardial perfusion SPECT and tri-plane tissue Doppler imaging

    PubMed Central

    Ajmone Marsan, Nina; Henneman, Maureen M.; Chen, Ji; Ypenburg, Claudia; Dibbets, Petra; Ghio, Stefano; Bleeker, Gabe B.; Stokkel, Marcel P.; van der Wall, Ernst E.; Tavazzi, Luigi; Garcia, Ernest V.

    2007-01-01

    Purpose To compare left ventricular (LV) dyssynchrony assessment by phase analysis from gated myocardial perfusion SPECT (GMPS) with LV dyssynchrony assessment by tri-plane tissue Doppler imaging (TDI). Baseline LV dyssynchrony assessed with standard deviation (SD) of time-to-peak systolic velocity of 12 LV segments (Ts-SD) with TDI has proven to be a powerful predictor of response to CRT. Information on LV dyssynchrony can also be provided by GMPS with phase analysis of regional LV maximal count changes throughout the cardiac cycle. Methods Forty heart failure patients, referred for evaluation of potential eligibility for CRT, underwent both 3D echocardiography, with tri-plane TDI, and resting GMPS. From tri-plane TDI, Ts-SD was used as a validated parameter of LV dyssynchrony and compared with different indices (histogram bandwidth, phase SD, histogram skewness and kurtosis) derived from phase analysis of GMPS. Results Histogram bandwidth and phase SD showed good correlation with Ts-SD (r=0.77 and r=0.74, p<0.0001, respectively). Patients with substantial LV dyssynchrony assessed with tri-plane TDI (Ts-SD ≥33 ms) had also significantly higher values of histogram bandwidth and phase SD. Conclusions The results of this study support the use of phase analysis by GMPS to evaluate LV dyssynchrony. Histogram bandwidth and phase SD showed the best correlation with Ts-SD assessed with tri-plane TDI and appeared the most optimal variables for assessment of LV dyssynchrony with GMPS. PMID:17874098

  10. Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy

    NASA Astrophysics Data System (ADS)

    Yu, Jiun-Yann; Kuo, Chun-Hung; Holland, Daniel B.; Chen, Yenyu; Ouyang, Mingxing; Blake, Geoffrey A.; Zadoyan, Ruben; Guo, Chin-Lin

    2011-11-01

    Optical sectioning provides three-dimensional (3D) information in biological tissues. However, most imaging techniques implemented with optical sectioning are either slow or deleterious to live tissues. Here, we present a simple design for wide-field multiphoton microscopy, which provides optical sectioning at a reasonable frame rate and with a biocompatible laser dosage. The underlying mechanism of optical sectioning is diffuser-based temporal focusing. Axial resolution comparable to confocal microscopy is theoretically derived and experimentally demonstrated. To achieve a reasonable frame rate without increasing the laser power, a low-repetition-rate ultrafast laser amplifier was used in our setup. A frame rate comparable to that of epifluorescence microscopy was demonstrated in the 3D imaging of fluorescent protein expressed in live epithelial cell clusters. In this report, our design displays the potential to be widely used for video-rate live-tissue and embryo imaging with axial resolution comparable to laser scanning microscopy.

  11. Automated Breast Volume Scanning: Identifying 3-D Coronal Plane Imaging Features May Help Categorize Complex Cysts.

    PubMed

    Wang, Hong-Yan; Jiang, Yu-Xin; Zhu, Qing-Li; Zhang, Jing; Xiao, Meng-Su; Liu, He; Dai, Qing; Li, Jian-Chu; Sun, Qiang

    2016-03-01

    The study described here sought to identify specific ultrasound (US) automated breast volume scanning (ABVS) features that distinguish benign from malignant lesions. Medical records of 750 patients with 792 breast lesions were retrospectively reviewed. Of the 750 patients, 101 with 122 cystic lesions were included in this study, and the results ABVS results were compared with biopsy pathology results. These lesions were classified into six categories based on ABVS sonographic features: type I = simple cyst; type II = clustered cyst; type III = cystic masses with thin septa; type IV = complex cyst; type V = predominantly cystic masses; and type VI = predominantly solid masses. Comparisons were conducted between the ABVS coronal plane features of the lesions and histopathology results, and the positive predictive value (PPV) was calculated for each feature. Of the 122 lesions, 90 (73.8%) were classified as benign, and 32 (26.2%) were classified as malignant. The sensitivity, specificity and accuracy associated with ABVS features for cystic lesions were 78.1%, 74.4% and 75.4%, respectively. The 11 cases (8.9%) of type I-IV cysts were all benign. Of the 22 (18.0%) type V cysts, 16 (13.1%) were benign and 6 (4.9%) were malignant. Of the 89 (72.9%) type VI cysts, 63 (51.7%) were benign and 26 (21.3%) were malignant. The typical symptoms of malignancy on ABVS include retraction (PPV = 100%, p < 0.05), hyper-echoic halos (PPV = 85.7%, p < 0.05), microcalcification (PPV = 66.7%, p < 0.05), thick walls or thick septa (PPV = 62.5%, p < 0.05), irregular shape (PPV: 51.2%, p < 0.05), indistinct margin (PPV: 48.6%, p < 0.05) and predominantly solid masses with eccentric cystic foci (PPV = 46.8%, p < 0.05). ABVS can reveal sonographic features of the lesions along the coronal plane, which may be of benefit in the detection of malignant, predominantly cystic masses and provide high clinical values.

  12. Electrostatics of a Point Charge between Intersecting Planes: Exact Solutions and Method of Images

    ERIC Educational Resources Information Center

    Mei, W. N.; Holloway, A.

    2005-01-01

    In this work, the authors present a commonly used example in electrostatics that could be solved exactly in a conventional manner, yet expressed in a compact form, and simultaneously work out special cases using the method of images. Then, by plotting the potentials and electric fields obtained from these two methods, the authors demonstrate that…

  13. Methyl green and nitrotetrazolium blue chloride co-expression in colon tissue: A hyperspectral microscopic imaging analysis

    NASA Astrophysics Data System (ADS)

    Li, Qingli; Liu, Hongying; Wang, Yiting; Sun, Zhen; Guo, Fangmin; Zhu, Jianzhong

    2014-12-01

    Histological observation of dual-stained colon sections is usually performed by visual observation under a light microscope, or by viewing on a computer screen with the assistance of image processing software in both research and clinical settings. These traditional methods are usually not sufficient to reliably differentiate spatially overlapping chromogens generated by different dyes. Hyperspectral microscopic imaging technology offers a solution for these constraints as the hyperspectral microscopic images contain information that allows differentiation between spatially co-located chromogens with similar but different spectra. In this paper, a hyperspectral microscopic imaging (HMI) system is used to identify methyl green and nitrotetrazolium blue chloride in dual-stained colon sections. Hyperspectral microscopic images are captured and the normalized score algorithm is proposed to identify the stains and generate the co-expression results. Experimental results show that the proposed normalized score algorithm can generate more accurate co-localization results than the spectral angle mapper algorithm. The hyperspectral microscopic imaging technology can enhance the visualization of dual-stained colon sections, improve the contrast and legibility of each stain using their spectral signatures, which is helpful for pathologist performing histological analyses.

  14. THE IMAGING PROPERTIES OF THE GAS PIXEL DETECTOR AS A FOCAL PLANE POLARIMETER

    SciTech Connect

    Fabiani, S.; Costa, E.; Del Monte, E.; Muleri, F.; Soffitta, P.; Rubini, A.; Bellazzini, R.; Brez, A.; De Ruvo, L.; Minuti, M.; Pinchera, M.; Sgró, C.; Spandre, G.; Spiga, D.; Tagliaferri, G.; Pareschi, G.; Basso, S.; Citterio, O.; Burwitz, V.; Burkert, W.; and others

    2014-06-01

    X-rays are particularly suited to probing the physics of extreme objects. However, despite the enormous improvements of X-ray astronomy in imaging, spectroscopy, and timing, polarimetry remains largely unexplored. We propose the photoelectric polarimeter Gas Pixel Detector (GPD) as a candidate instrument to fill the gap created by more than 30 yr without measurements. The GPD, in the focus of a telescope, will increase the sensitivity of orders of magnitude. Moreover, since it can measure the energy, the position, the arrival time, and the polarization angle of every single photon, it allows us to perform polarimetry of subsets of data singled out from the spectrum, the light curve, or an image of the source. The GPD has an intrinsic, very fine imaging capability, and in this work we report on the calibration campaign carried out in 2012 at the PANTER X-ray testing facility of the Max-Planck-Institut für extraterrestrische Physik of Garching (Germany) in which, for the first time, we coupled it with a JET-X optics module with a focal length of 3.5 m and an angular resolution of 18 arcsec at 4.5 keV. This configuration was proposed in 2012 aboard the X-ray Imaging Polarimetry Explorer (XIPE) in response to the ESA call for a small mission. We derived the imaging and polarimetric performance for extended sources like pulsar wind nebulae and supernova remnants as case studies for the XIPE configuration and also discuss possible improvements by coupling the detector with advanced optics that have a finer angular resolution and larger effective areas to study extended objects with more detail.

  15. Micromachined room-temperature microbolometer for millimeter-wave detection and focal-plane imaging arrays

    NASA Astrophysics Data System (ADS)

    Rahman, Arifur; Duerr, Erik K.; de Lange, Gert; Hu, Qing

    1997-06-01

    We have combined silicon micromachining technology with planar circuits to fabricated room-temperature niobium microbolometers for millimeter-wave detection. In this type of detector, a thin niobium film, with a dimension much smaller than the wavelength, is fabricated on a 1-micrometers thick Si3N4 membrane of square and cross geometries. The Nb film acts both as a radiation absorber and temperature sensor. Incident radiation is coupled into the microbolometer by a 0.37 (lambda) dipole antenna with a center frequency of 95 GHz and a 3-db bandwidth of 15%, which is impedance matched with the Nb film. The dipole antennas is placed inside a micromachined pyramidal cavity formed by anisotropically etched Si wafers. To increase the Gaussian beam coupling efficiency, a machined square or circular horn is placed in front of the micromachined section. Circular horns interface more easily with die-based manufacturing processes; therefore, we have developed simulation tools that allow us to model circular machined horns. We have fabricated both single element receivers and 3 X 3 focal-plane arrays using uncooled Nb microbolometers. An electrical NEP level of 8.3 X 10-11 W/(root)Hz has been achieved for a single- element receiver. This NEP level is better than that of the commercial room-temperature pyroelectric millimeter-wave detectors. The frequency response of the microbolometer has a ln(1/f) dependence with frequency, and the roll-off frequency is approximately 35 kHz.

  16. Joint Image and Pupil Plane Reconstruction Algorithm based on Bayesian Techniques

    DTIC Science & Technology

    2007-12-01

    a system would also potentially allow for the formation of large synthetic apertures. The algorithm developed used many frames of coherent pupil and...1.2.1 Deconvolution . . . . . . . . . . . . . . . . . . . 2 1.2.2 Phase Retrieval/Imaging Correlography . . . . . 2 1.2.3 Prior Synthetic Aperture...Augmentation of existing systems . . . . . . . . 87 7.4.2 Conformal Arrays . . . . . . . . . . . . . . . . . 88 7.4.3 Synthetic aperture LADAR

  17. 3D coaxial out-of-plane metallic antennas for filtering and multi-spectral imaging in the infrared range.

    PubMed

    Jacassi, Andrea; Bozzola, Angelo; Zilio, Pierfrancesco; Tantussi, Francesco; De Angelis, Francesco

    2016-06-27

    We fabricated and investigated a new configuration of 3D coaxial metallic antennas working in the infrared which combines the strong lateral light scattering of vertical plasmonic structures with the selective spectral transmission of 2D arrays of coaxial apertures. The coaxial structures are fabricated with a top-down method based on a template of hollow 3D antennas. Each antenna has a multilayer radial structure consisting of dielectric and metallic materials not achievable in a 2D configuration. A planar metallic layer is inserted normally to the antennas. The outer dielectric shell of the antenna defines a nanometric gap between the horizontal plane and the vertical walls. Thanks to this aperture, light can tunnel to the other side of the plane, and be transmitted to the far field in a set of resonances. These are investigated with finite-elements electromagnetic calculations and with Fourier-transform infrared spectroscopy measurements. The spectral position of the resonances can be tuned by changing the lattice period and/or the antenna length. Thanks to the strong scattering provided by the 3D geometry, the transmission peaks possess a high signal-to-noise ratio even when the illuminated area is less than 2 × 2 times the operation wavelength. This opens new possibilities for multispectral imaging in the IR with wavelength-scale spatial resolution.

  18. 3D coaxial out-of-plane metallic antennas for filtering and multi-spectral imaging in the infrared range

    PubMed Central

    Jacassi, Andrea; Bozzola, Angelo; Zilio, Pierfrancesco; Tantussi, Francesco; De Angelis, Francesco

    2016-01-01

    We fabricated and investigated a new configuration of 3D coaxial metallic antennas working in the infrared which combines the strong lateral light scattering of vertical plasmonic structures with the selective spectral transmission of 2D arrays of coaxial apertures. The coaxial structures are fabricated with a top-down method based on a template of hollow 3D antennas. Each antenna has a multilayer radial structure consisting of dielectric and metallic materials not achievable in a 2D configuration. A planar metallic layer is inserted normally to the antennas. The outer dielectric shell of the antenna defines a nanometric gap between the horizontal plane and the vertical walls. Thanks to this aperture, light can tunnel to the other side of the plane, and be transmitted to the far field in a set of resonances. These are investigated with finite-elements electromagnetic calculations and with Fourier-transform infrared spectroscopy measurements. The spectral position of the resonances can be tuned by changing the lattice period and/or the antenna length. Thanks to the strong scattering provided by the 3D geometry, the transmission peaks possess a high signal-to-noise ratio even when the illuminated area is less than 2 × 2 times the operation wavelength. This opens new possibilities for multispectral imaging in the IR with wavelength-scale spatial resolution. PMID:27345517

  19. 640 x 512 Pixels Long-Wavelength Infrared (LWIR) Quantum-Dot Infrared Photodetector (QDIP) Imaging Focal Plane Array

    NASA Technical Reports Server (NTRS)

    Gunapala, Sarath D.; Bandara, Sumith V.; Hill, Cory J.; Ting, David Z.; Liu, John K.; Rafol, Sir B.; Blazejewski, Edward R.; Mumolo, Jason M.; Keo, Sam A.; Krishna, Sanjay; Chang, Y. -C.; Shott, Craig A.

    2007-01-01

    Epitaxially grown self-assembled. InAs-InGaAs-GaAs quantum dots (QDs) are exploited for the development of large-format long-wavelength infrared focal plane arrays (FPAs). The dot-in-a-well (DWELL) structures were experimentally shown to absorb both 45 degrees and normal incident light, therefore, a reflection grating structure was used to enhance the quantum efficiency. The devices exhibit peak responsivity out to 8.1 micrometers, with peak detectivity reaching approximately 1 X 10(exp 10) Jones at 77 K. The devices were fabricated into the first long-wavelength 640 x 512 pixel QD infrared photodetector imaging FPA, which has produced excellent infrared imagery with noise equivalent temperature difference of 40 mK at 60-K operating temperature.

  20. In-plane strain measurements on a microscopic scale by coupling digital image correlation and an in situ SEM technique

    SciTech Connect

    Lagattu, Fabienne . E-mail: lagattu@lmpm.ensma.fr; Bridier, Florent; Villechaise, Patrick; Brillaud, Jean

    2006-01-15

    The purpose of this paper is to present a method based on the correlation of digital images obtained on a microscopic scale. A specific grainy pattern has been developed. The use of the scanning electron microscopy (SEM) allowed the determination of full-field 2D displacements on an object surface with a spatial resolution of about 1 {mu}m. Validation tests were performed in order to quantify performances and limits of this method. An example of its application is presented for a Ti-6Al-4V titanium alloy. Results show that it is possible to obtain in-plane displacement values on the object surface with efficient spatial resolution and accuracy. Thus, such a technique can be used to highlight on a relevant scale the role of the microstructure in material deformation processes.

  1. Nested object watermarking: comparison of block-luminance and blue channel LSB wet paper code image watermarking

    NASA Astrophysics Data System (ADS)

    Vielhauer, Claus; Dittmann, Jana

    2007-02-01

    Annotation watermarking (sometimes also called caption or illustration watermarking) denotes a specific application of watermarks, which embeds supplementary information directly in the media, so that additional information is intrinsically linked to media content and does not get separated from the media by non-malicious processing steps such as image cropping or compression. Recently, nested object annotation watermarking (NOAWM) has been introduced as a specialized annotation watermarking domain, whereby hierarchical object information is embedded in photographic images. In earlier work, the Hierarchical Graph Concept (HGC) has been suggested as a first approach to model object relations, which are defined by users during editing processes, into a hierarchical tree structure. The original HGC method uses a code-book decomposition of the annotation tree and a block-luminance algorithm for embedding. In this article, two new approaches for embedding nested object annotations are presented and experimentally compared to the original HGC approach. The first one adopts the code-book scheme of HGC using an alternative embedding based on Wet Paper Codes in blue-channel LSB domain, whereas the second suggests a new method based on the concept of intrinsic signal inheritance by sub-band energy and phase modulation of image luminance blocks. A comparative experimental evaluation based on more than 100 test images is presented in the paper, whereby aspects of transparency and robustness with respect to the most relevant image modifications to annotations, cropping and JPEG compression, are discussed comparatively for the two code-book schemes and the novel inheritance approach.

  2. Focal plane array detectors with micro-bolometer structure and its application in IR and THz imaging

    NASA Astrophysics Data System (ADS)

    Wang, Jun; Mou, Wenchao; Gou, Jun; Jiang, Yadong

    2016-10-01

    Focal Plane Array (FPA) detector has characteristics of low cost, operating at room temperature, compatibility with the silicon CMOS technology, and high detecting performance, therefore it becomes a hot spot in infrared (IR) or terahertz (THz) detect field recently. However, the tradition structure of micro-bolometer has the conflict of the pixel size and thermal performance. In order to improve the detecting performance of small pixel size bolometer, high fill factor and low thermal conductance design should be considered. In IR detecting, double layers structure is an efficient method to improve the absorption of micro-bolometer and reduce thermal conductance. The three-dimension model of small size micro-bolometer was built in this article. The thermal and mechanical characters of those models were simulated and optimized, and finally the double layer structure micro-bolometer was fabricated with multifarious semiconductor recipes on the readout integrated chip wafer. For THz detecting, to improve the detecting performance, different dimension THz detectors based on micro-bridge structure were designed and fabricated to get optimizing micro-bolometer parameters from the test results of membrane deformation. A nanostructured titanium thin film absorber is integrated in the micro-bridge structure of the VOx micro-bolometer to enhance the absorption of THz radiation. Continuous-wave THz detection and imaging are demonstrated with a 2.52 THz far infrared CO2 laser and fabricated 320×240 vanadium oxide micro-bolometer focal plane array with optimized cell structure. With this detecting system, THz imaging of metal concealed in wiping cloth and envelope is demonstrated.

  3. Real-Time Terahertz Imaging Using a Quantum Cascade Laser and Uncooled Microbolometer Focal Plane Array

    DTIC Science & Technology

    2008-06-01

    1] D. Arnone, C. Ciesla, and M. Pepper , “Terahertz imaging comes into view,” Physics World 4, 35-40 (2000). [2] D. M. Mittleman, M. Gupta, R...of the 26th Annu. Int. Conf. of the IEEE (IEEE, 2004), pp. 1302-1305. [16] B. Ferguson , S. Wang, D. Gray, D. Abbott and X. -C. Zhang...using Kirchhoff migration," Opt. Lett. 26, 1513-1515 (2001). [30] B. Ferguson , S. Wang, D. Gray, D. Abbot, and X.-C. Zhang, "T-ray computed

  4. AlGaN-based focal plane arrays for selective UV imaging at 310nm and 280nm and route toward deep UV imaging

    NASA Astrophysics Data System (ADS)

    Reverchon, Jean-Luc; Robo, Jean-Alexandre; Truffer, Jean-Patrick; Caumes, Jean-Pascal; Mourad, Idir; Brault, Julien; Duboz, Jean-Yves

    2007-10-01

    The fast development of nitrides has given the opportunity to investigate AlGaN as a material for ultraviolet detection. Such camera present an intrinsic spectral selectivity and an extremely low dark current at room temperature. It can compete with technologies based on photocathodes, MCP intensifiers, back thinned CCD or hybrid CMOS focal plane arrays (FPA) for low flux measurements. AlGaN based cameras allow UV imaging without filters or with simplified ones in harsh solar blind conditions. Few results on camera have been shown in the last years, but the ultimate performances of AlGaN photodiodes couldn't be achieved due to parasitic illumination of multiplexers, responsivity of p layers in p-i-n structures, or use of cooled readout circuit. Such issues have prevented up to now a large development of this technology. We present results on focal plane array of 320x256 pixels with a pitch of 30μm for which Schottky photodiodes are multiplexed with a readout circuit protected by black matrix at room temperature. Theses focal plane present a peak reponsivity around 280nm and 310nm with a rejection of visible light of four decades only limited by internal photoemission in contact. Then we will show the capability to outdoor measurements. The noise figure is due to readout noise of the multiplexer and we will investigate the ultimate capabilities of Schottky diodes or Metal- Semiconductor-Metal (MSM) technologies to detect extremely low signal. Furthermore, we will consider deep UV measurements on single pixels MSM from 32nm to 61nm in a front side illumination configuration. Finally, we will define technology process allowing backside illumination and deep UV imaging.

  5. Red, green, and blue gray-value shift-based approach to whole-field imaging for tissue diagnostics.

    PubMed

    Valiyambath Krishnan, Mohankumar; Vadakke Matham, Murukeshan; Krishnan, Sathiyamoorthy; Parasuraman, Padmanabhan; Joseph, James; Bhakoo, Kishore

    2012-07-01

    Identification of abnormal pathology in situ remains one of the challenges of medicine. The interpretation of tissue conditions relies mainly on optical assessment, which can be difficult due to inadequate visual differences or poor color delineation. We propose a methodology to identify regions of abnormal tissue in a targeted area based on red, green, blue (RGB) shift analysis employing a simple CCD color camera and light-emitting diode illumination in a whole-field-imaging scheme. The concept involves analysis of RGB components in an image with respect to a reference set of RGB values under different illumination wavelengths. The magnitude of the gray value shift is estimated by calculating the Euclidean distance between their normalized RGB coordinates. The shift values obtained using these concepts are thereafter used to construct pseudo-colored images with high contrast, enabling easy identification of abnormal areas in the tissue. Images processed from experiments conducted with excised Wistar rat colon sample (lightly doped with Alexafluor 488) and with simulated tumor (cancer cell pellet placed on colon) showed clear localization of tumor region. This proposed approach and methodology is expected to find potential applications for the in vivo diagnosis of disease.

  6. Red, green, and blue gray-value shift-based approach to whole-field imaging for tissue diagnostics

    NASA Astrophysics Data System (ADS)

    Valiyambath Krishnan, Mohankumar; Vadakke Matham, Murukeshan; Krishnan, Sathiyamoorthy; Parasuraman, Padmanabhan; Joseph, James; Bhakoo, Kishore

    2012-07-01

    Identification of abnormal pathology in situ remains one of the challenges of medicine. The interpretation of tissue conditions relies mainly on optical assessment, which can be difficult due to inadequate visual differences or poor color delineation. We propose a methodology to identify regions of abnormal tissue in a targeted area based on red, green, blue (RGB) shift analysis employing a simple CCD color camera and light-emitting diode illumination in a whole-field-imaging scheme. The concept involves analysis of RGB components in an image with respect to a reference set of RGB values under different illumination wavelengths. The magnitude of the gray value shift is estimated by calculating the Euclidean distance between their normalized RGB coordinates. The shift values obtained using these concepts are thereafter used to construct pseudo-colored images with high contrast, enabling easy identification of abnormal areas in the tissue. Images processed from experiments conducted with excised Wistar rat colon sample (lightly doped with Alexafluor 488) and with simulated tumor (cancer cell pellet placed on colon) showed clear localization of tumor region. This proposed approach and methodology is expected to find potential applications for the in vivo diagnosis of disease.

  7. Focal Plane Detectors for the Advanced Gamma-Ray Imaging System (AGIS)

    SciTech Connect

    Otte, A. N.; Williams, D. A.; Byrum, K.; Drake, G.; Horan, D.; Smith, A.; Wagner, R. G.; Falcone, A.; Funk, S.; Tajima, H.; Mukherjee, R.

    2008-12-24

    The Advanced Gamma-Ray Imaging System (AGIS) is a concept for the next generation observatory in ground-based very high energy gamma-ray astronomy. Design goals are ten times better sensitivity, higher angular resolution, and a lower energy threshold than existing Cherenkov telescopes. Simulations show that a substantial improvement in angular resolution may be achieved if the pixel diameter is reduced to the order of 0.05 deg, i.e. two to three times smaller than the pixel diameter of current Cherenkov telescope cameras. At these dimensions, photon detectors with smaller physical dimensions can be attractive alternatives to the classical photomultiplier tube (PMT). Furthermore, the operation of an experiment with the size of AGIS requires photon detectors that are among other things more reliable, more durable, and possibly higher efficiency photon detectors. Alternative photon detectors we are considering for AGIS include both silicon photomultipliers (SiPMs) and multi-anode photomultipliers (MAPMTs). Here we present results from laboratory testing of MAPMTs and SiPMs along with results from the first incorporation of these devices into cameras on test bed Cherenkov telescopes.

  8. Synchrotron Infrared Confocal Microspectroscopic Spatial Resolution or a Customized Synchrotron/focal Plane Array System Enhances Chemical Imaging of Biological Tissue or Cells

    SciTech Connect

    D Wetzel; M Nasse; =

    2011-12-31

    Spectroscopy and spatially resolved chemical imaging of biological materials using an infrared microscope is greatly enhanced with confocal image plane masking to 5-6 {mu} with a third generation microspectrometer and illumination with a synchrotron radiation source compared to globar illuminated and array detection or singly masked system. Steps toward this instrumental achievement are illustrated with spectra and images of biological tissue sections, including single cells, brain, aorta, and grain specimens. A recent, customized synchrotron infrared microspectrometer installation enables focal plane array detection to achieve both rapid and high definition chemical imaging. Localization of the ester carbonyl population in single modified starch granules was used to provide direct comparison of the two advanced imaging capabilities.

  9. Synchrotron infrared confocal microspectroscopic spatial resolution or a customized synchrotron/focal plane array system enhances chemical imaging of biological tissue or cells

    NASA Astrophysics Data System (ADS)

    Wetzel, David L.; Nasse, Michael J.

    2011-09-01

    Spectroscopy and spatially resolved chemical imaging of biological materials using an infrared microscope is greatly enhanced with confocal image plane masking to 5-6 μm with a third generation microspectrometer and illumination with a synchrotron radiation source compared to globar illuminated and array detection or singly masked system. Steps toward this instrumental achievement are illustrated with spectra and images of biological tissue sections, including single cells, brain, aorta, and grain specimens. A recent, customized synchrotron infrared microspectrometer installation enables focal plane array detection to achieve both rapid and high definition chemical imaging. Localization of the ester carbonyl population in single modified starch granules was used to provide direct comparison of the two advanced imaging capabilities.

  10. Elasticity imaging of arterial wall with transcutaneous ultrasound both in longitudinal-axis and short-axis planes

    NASA Astrophysics Data System (ADS)

    Kanai, Hiroshi; Hasegawa, Hideyuki

    2001-05-01

    A method for measuring regional elasticity of tissue surrounding atherosclerotic plaque is described. An ultrasonic beam was scanned with a conventional linear-type probe, and multiple layers were preset from luminal surface to adventitia of the common carotid artery (CCA) with intervals of 375 μm. By applying the method [IEEE Trans. UFFC 46, 1229-1241 (1999)], a minute decrease of several tenths of a micrometer in thickness of each layer resulting from arrival of the pressure wave was determined. By assuming that the arterial wall is incompressible and that the blood pressure is applied normal to each layer, the elastic modulus in the circumferential direction of each layer was estimated at intervals of 75 μm in the radial direction and 150-300 μm in longitudinal direction. On the other hand, by designing the directions of ultrasonic beams so that each beam always passes through the center of the artery, the cross-sectional elasticity image in the short-axis plane was also obtained. Based on the elasticity library determined by comparing the elasticity distribution and their pathological images, each point was statistically categorized as lipid, a mixture of smooth muscle and collagen fiber, or other. By applying the method to the CCAs, soft inclusion of lipid was found for plaques.

  11. In-plane vibration characterization of microelectromechanical systems using acousto-optic modulated partially incoherent stroboscopic imaging

    NASA Astrophysics Data System (ADS)

    Wang, Dung-An; Sheu, Fang-Wen; Chiu, Yen-Sih

    2011-07-01

    A technique using acousto-optic modulated partially incoherent stroboscopic imaging for measurement of in-plane motion of microelectromechanical systems (MEMS) is presented. Vibration measurement is allowed by using flashes of the partially incoherent light source to freeze the positions of the microstructure at 12 equally spaced phases of the vibration period. The first-order diffracted beam taken out by an acousto-optic modulator (AOM) from the light beam of a laser is made partially incoherent by a rotating diffuser and then serves as the stroboscopic light source. Both the MEMS excitation signal and the flash control signal are provided by a dual-channel function generator. The main advantage of this measurement method is the absence of a stroboscopic generator and a high speed digital camera. Microscale prototypes are fabricated and tested. Quantitative estimates of the harmonic responses of the prototypes are obtained from the recorded images. The results agree with those obtained with a commercial MEMS motion analyzer TM with relative errors less than 2%.

  12. An Indium Gallium Arsenide Visible/SWIR Focal Plane Array for Low Light Level Imaging

    NASA Technical Reports Server (NTRS)

    Cohen, Marshall J.; Ettenberg, Martin H.; Lange, Michael J.; Olsen, Gregory H.

    1999-01-01

    PIN photodiodes fabricated from indium gallium arsenide lattice-matched to indium phosphide substrates (In(.53)Ga(.47)As/InP) exhibit low reverse saturation current densities (JD < 10(exp -8) A/sq cm), and high shunt resistance-area products (RoA > 10(exp 6) omega-sq cm) at T=290K. Backside-illuminated, hybrid-integrated InGaAs FPAs are sensitive from 0.9 micrometers to 1.7 micrometers. 290K detectivities, D(*), greater than 10(exp 14) cm-(square root of Hz/W) are demonstrated. This represents the highest room temperature detectivity of any infrared material. The long wavelength cutoff (1.7 micrometers) makes In(.53)Ga(.47)As an idea match to the available airglow that has major peaks at 1.3 micrometers and 1.6 micrometers. The short wavelength 'cut-on' at 0.9 micrometers is due to absorption in the InP substrate. We will report on new InGaAs FPA epitaxial structures and processing techniques. These have resulted in improved performance in the form of a 10 x increase in detectivity and visible response via removal of the InP substrate. The resulting device features visible and SWIR response with greater than 15% quantum efficiency at 0.5 micrometers while maintaining the long wavelength cutoff. Imaging has been demonstrated under overcast starlight/urban glow conditions with cooling provided by a single stage thermoelectric cooler. Details on the material structure and device fabrication, quantitative characterization of spectral response and detectivity, as well as examples of night vision imagery are presented.

  13. Compact plane illumination plugin device to enable light sheet fluorescence imaging of multi-cellular organisms on an inverted wide-field microscope

    PubMed Central

    Guan, Zeyi; Lee, Juhyun; Jiang, Hao; Dong, Siyan; Jen, Nelson; Hsiai, Tzung; Ho, Chih-Ming; Fei, Peng

    2015-01-01

    We developed a compact plane illumination plugin (PIP) device which enabled plane illumination and light sheet fluorescence imaging on a conventional inverted microscope. The PIP device allowed the integration of microscope with tunable laser sheet profile, fast image acquisition, and 3-D scanning. The device is both compact, measuring approximately 15 by 5 by 5 cm, and cost-effective, since we employed consumer electronics and an inexpensive device molding method. We demonstrated that PIP provided significant contrast and resolution enhancement to conventional microscopy through imaging different multi-cellular fluorescent structures, including 3-D branched cells in vitro and live zebrafish embryos. Imaging with the integration of PIP greatly reduced out-of-focus contamination and generated sharper contrast in acquired 2-D plane images when compared with the stand-alone inverted microscope. As a result, the dynamic fluid domain of the beating zebrafish heart was clearly segmented and the functional monitoring of the heart was achieved. Furthermore, the enhanced axial resolution established by thin plane illumination of PIP enabled the 3-D reconstruction of the branched cellular structures, which leads to the improvement on the functionality of the wide field microscopy. PMID:26819828

  14. Red, green, and blue luminescence by carbon dots: full-color emission tuning and multicolor cellular imaging.

    PubMed

    Jiang, Kai; Sun, Shan; Zhang, Ling; Lu, Yue; Wu, Aiguo; Cai, Congzhong; Lin, Hengwei

    2015-04-27

    A facile approach for preparation of photoluminescent (PL) carbon dots (CDs) is reported. The three resulting CDs emit bright and stable red, green and blue (RGB) colors of luminescence, under a single ultraviolet-light excitation. Alterations of PL emission of these CDs are tentatively proposed to result from the difference in their particle size and nitrogen content. Interestingly, up-conversion (UC)PL of these CDs is also observed. Moreover, flexible full-color emissive PVA films can be achieved through mixing two or three CDs in the appropriate ratios. These CDs also show low cytotoxicity and excellent cellular imaging capability. The facile preparation and unique optical features make these CDs potentially useful in numerous applications such as light-emitting diodes, full-color displays, and multiplexed (UC)PL bioimaging.

  15. Visualization of Venous Compliance of Superficial Veins Using Non-Contact Plethysmography Based on Digital Red-Green-Blue Images

    PubMed Central

    Nakano, Kazuya; Aoki, Yuta; Satoh, Ryota; Suzuki, Hiroyuki; Nishidate, Izumi

    2016-01-01

    We propose the visualization of venous compliance (VC) using a digital red-green-blue (RGB) camera. The new imaging method, which transforms RGB values into VC, combines VC evaluation with blood concentration estimation from the RGB values of each pixel. We evaluate a non-contact plethysmography (NCPG) system for VC based on comparisons with conventional strain gauge plethysmography (SPG). We conduct in vivo measurements using both systems and investigate their differences by evaluating the VC. The results show that the two methods measure different blood vessels and that errors caused by interstitial fluid accumulation are negligible for the NCPG system, whereas SPG is influenced by such errors. Additionally, we investigate the relationship between VC and physical activity using NCPG. PMID:27897981

  16. Identification and Quantification of Microplastics in Wastewater Using Focal Plane Array-Based Reflectance Micro-FT-IR Imaging.

    PubMed

    Tagg, Alexander S; Sapp, Melanie; Harrison, Jesse P; Ojeda, Jesús J

    2015-06-16

    Microplastics (<5 mm) have been documented in environmental samples on a global scale. While these pollutants may enter aquatic environments via wastewater treatment facilities, the abundance of microplastics in these matrices has not been investigated. Although efficient methods for the analysis of microplastics in sediment samples and marine organisms have been published, no methods have been developed for detecting these pollutants within organic-rich wastewater samples. In addition, there is no standardized method for analyzing microplastics isolated from environmental samples. In many cases, part of the identification protocol relies on visual selection before analysis, which is open to bias. In order to address this, a new method for the analysis of microplastics in wastewater was developed. A pretreatment step using 30% hydrogen peroxide (H2O2) was employed to remove biogenic material, and focal plane array (FPA)-based reflectance micro-Fourier-transform (FT-IR) imaging was shown to successfully image and identify different microplastic types (polyethylene, polypropylene, nylon-6, polyvinyl chloride, polystyrene). Microplastic-spiked wastewater samples were used to validate the methodology, resulting in a robust protocol which was nonselective and reproducible (the overall success identification rate was 98.33%). The use of FPA-based micro-FT-IR spectroscopy also provides a considerable reduction in analysis time compared with previous methods, since samples that could take several days to be mapped using a single-element detector can now be imaged in less than 9 h (circular filter with a diameter of 47 mm). This method for identifying and quantifying microplastics in wastewater is likely to provide an essential tool for further research into the pathways by which microplastics enter the environment.

  17. Determining the Instrumental Rotation Rate of MWO's 60' Tower Image Plane and Its Impact on Results from Ring-Diagram Analysis

    NASA Astrophysics Data System (ADS)

    Pinkerton I., S. F.; Rhodes, E. J., Jr.; Bogart, R. S.; Orr, M.; Martin, G.; Spinella, A.

    2013-12-01

    Time series of full-disk Dopplergrams were acquired at the 60-Foot Solar tower of the Mount Wilson Observatory every year between 1987 and 2009. The 60-Foot Tower was designed by George Ellery Hale to provide an image plane that did not rotate throughout each observing day. However, preliminary analyses of a portion of this archive, carried out in 2007, suggested that the focal plane of the Tower might actually be rotating slowly. If confirmed, such an instrumental rotation would suggest that the alignment of the optics has changed slightly over the years since 1907. This possible rotation of the image plane was uncovered through the computation of subsurface flow maps using the ring-diagram method of local helioseismology. Some of the initial MWO flow maps appeared to show evidence for a so-called “washing machine” effect similar to the pattern that was seen in the initial GONG flow maps. We have been working to confirm the early estimates of the focal plane rotation. The purpose of this paper is to report on the status of this endeavor as well as explore the ramifications of a rotating image plane on our anticipated meridional and zonal flow results.

  18. A novel Region of Interest (ROI) imaging technique for biplane imaging in interventional suites: high-resolution small field-of-view imaging in the frontal plane and dose-reduced, large field-of-view standard-resolution imaging in the lateral plane

    PubMed Central

    Swetadri Vasan, S.N; Ionita, C.; Bednarek, D.R; Rudin, S.

    2014-01-01

    Endovascular-Image-Guided-Interventional (EIGI) treatment of neuro-vascular conditions such as aneurysms, stenosed arteries, and vessel thrombosis make use of treatment devices such as stents, coils, and balloons which have very small feature sizes, 10's of microns to a few 100's of microns, and hence demand a high resolution imaging system. The current state-of-the-art flat panel detector (FPD) has about a 200-um pixel size with the Nyquist of 2.5 lp/mm. For higher-resolution imaging a charge-coupled device (CCD) based Micro-Angio -Fluoroscope (MAF-CCD) with a pixel size of 35um (Nyquist of 11 lp/mm) was developed and previously reported. Although the detector addresses the high resolution needs, the Field-Of-View (FOV) is limited to 3.5 cm × 3.5 cm, which is much smaller than current FPDs. During the use of the MAF-CCD for delicate parts of the intervention, it may be desirable to have real-time monitoring outside the MAF FOV with a low dose, and lower, but acceptable, quality image. To address this need, a novel imaging technique for biplane imaging systems has been developed, using an MAF-CCD in the frontal plane and a dose-reduced standard large FOV imager in the lateral plane. The dose reduction is achieved by using a combination of ROI fluoroscopy and spatially different temporal filtering, a technique that has been previously presented. In order to evaluate this technique, a simulation using images acquired during an actual EIGI treatment on a patient, followed by an actual implementation on phantoms is presented. PMID:25302001

  19. A novel Region of Interest (ROI) imaging technique for biplane imaging in interventional suites: high-resolution small field-of-view imaging in the frontal plane and dose-reduced, large field-of-view standard-resolution imaging in the lateral plane.

    PubMed

    Swetadri Vasan, S N; Ionita, C; Bednarek, D R; Rudin, S

    2014-03-19

    Endovascular-Image-Guided-Interventional (EIGI) treatment of neuro-vascular conditions such as aneurysms, stenosed arteries, and vessel thrombosis make use of treatment devices such as stents, coils, and balloons which have very small feature sizes, 10's of microns to a few 100's of microns, and hence demand a high resolution imaging system. The current state-of-the-art flat panel detector (FPD) has about a 200-um pixel size with the Nyquist of 2.5 lp/mm. For higher-resolution imaging a charge-coupled device (CCD) based Micro-Angio -Fluoroscope (MAF-CCD) with a pixel size of 35um (Nyquist of 11 lp/mm) was developed and previously reported. Although the detector addresses the high resolution needs, the Field-Of-View (FOV) is limited to 3.5 cm × 3.5 cm, which is much smaller than current FPDs. During the use of the MAF-CCD for delicate parts of the intervention, it may be desirable to have real-time monitoring outside the MAF FOV with a low dose, and lower, but acceptable, quality image. To address this need, a novel imaging technique for biplane imaging systems has been developed, using an MAF-CCD in the frontal plane and a dose-reduced standard large FOV imager in the lateral plane. The dose reduction is achieved by using a combination of ROI fluoroscopy and spatially different temporal filtering, a technique that has been previously presented. In order to evaluate this technique, a simulation using images acquired during an actual EIGI treatment on a patient, followed by an actual implementation on phantoms is presented.

  20. A novel Region of Interest (ROI) imaging technique for biplane imaging in interventional suites: high-resolution small field-of-view imaging in the frontal plane and dose-reduced, large field-of-view standard-resolution imaging in the lateral plane

    NASA Astrophysics Data System (ADS)

    Swetadri Vasan, Setlur Nagesh; Ionita, C.; Bednarek, D. R.; Rudin, Stephen

    2014-03-01

    Endovascular-Image-Guided-Interventional (EIGI) treatment of neuro-vascular conditions such as aneurysms, stenosed arteries, and vessel thrombosis make use of treatment devices such as stents, coils, and balloons which have very small feature sizes, 10's of microns to a few 100's of microns, and hence demand a high resolution imaging system. The current state-of-the-art flat panel detector (FPD) has about a 200-um pixel size with the Nyquist of 2.5 lp/mm. For higher-resolution imaging a charge-coupled device (CCD) based Micro-Angio - Fluoroscope (MAF-CCD) with a pixel size of 35um (Nyquist of 11 lp/mm) was developed and previously reported. Although the detector addresses the high resolution needs, the Field-Of-View (FOV) is limited to 3.5 cm x 3.5 cm, which is much smaller than current FPDs. During the use of the MAF-CCD for delicate parts of the intervention, it may be desirable to have real-time monitoring outside the MAF FOV with a low dose, and lower, but acceptable, quality image. To address this need, a novel imaging technique for biplane imaging systems has been developed, using an MAFCCD in the frontal plane and a dose-reduced standard large FOV imager in the lateral plane. The dose reduction is achieved by using a combination of ROI fluoroscopy and spatially different temporal filtering, a technique that has been previously presented. In order to evaluate this technique, a simulation using images acquired during an actual EIGI treatment on a patient, followed by an actual implementation on phantoms is presented.

  1. Use digital subtraction images of blue-light and near-infrared autofluorescence for the assessment of irregular foveal contour.

    PubMed

    Hua, Rui; Gangwani, Rita; Liu, Limin; Chen, Lei

    2015-01-01

    The aims of this study are to generate subtraction images of blue-light autofluorescence (BL-AF) and near-infrared autofluorescence (NIR-AF) from normal eyes, eyes with full thickness macular holes, and eyes with irregular foveal contour, and to compare their autofluorescence patterns. This retrospective study included 44 normal eyes of 22 health individuals, 32 eyes with full thickness macular holes of 32 patients, and 36 eyes with irregular foveal contour of 36 patients. BL-AF and NIR-AF were obtained from all patients and used to generate subtraction images using the Image J software. The decreased signal of central patch was recorded. The central foveal thickness (CFT) and outer nucleus layer (ONL) thickness of fovea were measured to calculate the ONL thickness/CFT ratio. The subtraction images showed regularly increased signal in the central macula of all normal eyes. In contrast, decreased signal of central patch was detected in all full thickness macular holes eyes and 26 out of 36 eyes with irregular foveal contour. No significant difference of the ONL thickness/CFT ratio (F = 2.32, P = 0.113) was observed between normal and irregular foveal contour eyes with or without decreased signal of central patch. Both regularly increased signal and decreased signal of central patch were detected in the eyes with irregular foveal contour. Our results suggest that subtraction images are useful for the assessment of certain macular conditions by providing supplementary information to the green-light autofluorescence and BL-AF.

  2. An Evaluation of the Instruction Carried out with Printed Laboratory Materials Designed in Accordance with 5E Model: Reflection of Light and Image on a Plane Mirror

    ERIC Educational Resources Information Center

    Ayvaci, Hakan Sevki; Yildiz, Mehmet; Bakirci, Hasan

    2015-01-01

    This study employed a print laboratory material based on 5E model of constructivist learning approach to teach reflection of light and Image on a Plane Mirror. The effect of the instruction which conducted with the designed print laboratory material on academic achievements of prospective science and technology teachers and their attitudes towards…

  3. Axial Plane Optical Microscopy

    PubMed Central

    Li, Tongcang; Ota, Sadao; Kim, Jeongmin; Wong, Zi Jing; Wang, Yuan; Yin, Xiaobo; Zhang, Xiang

    2014-01-01

    We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues. PMID:25434770

  4. Design, development, characterization and qualification of infrared focal plane area array detectors for space-borne imaging applications

    NASA Astrophysics Data System (ADS)

    Jain, Ankur; Banerjee, Arup

    2016-05-01

    This paper discusses the design, development, characterization and qualification aspects of large format Infrared Focal Plane Arrays (IRFPA) required for panchromatic, multi-, hyper- and ultra-spectral imaging applications from a space-borne imager. Detection of feeble radiant flux from the intended target in narrow spectral bands requires a highly sensitive low noise sensor array with high well capacity. For this the photodiode arrays responsive in desired spectral band are grown using different growth techniques and flip-chip bonded with a suitable Si Read-out ICs (ROICs) for signal conditioning. IR detectors require cryogenic cooling to achieve background limited performance. Although passive radiative cooling is always the preferred choice of cooling in space, it is not suitable for cooling IRFPAs due to high thermal loads. To facilitate characterization of IRFPAs and cool them to desired cryogenic temperature, an Integrated Detector Dewar Cooler Assembly (IDDCA) is essential where the detector array sits over the cold tip of an active cooler and the detector cooler assembly is vacuum sealed in a thermally isolated Dewar. A cold shield above the sensor array inside the Dewar restricts its field-of-view and a cold filter fine tunes its spectral response. In this paper, various constituents of an IRFPA like sensor array materials, growth techniques, ROICs, filters, cold shields, cooling techniques etc., their types and selection criteria for different applications are discussed in detail. Design aspects of IRFPA characterization test bench, challenges involved in radiometric and spectral characterization and space qualification of such IDDCA based IRFPAs are also discussed.

  5. Real-Time Intraoperative Detection of Breast Cancer using Near-infrared Fluorescence Imaging and Methylene Blue

    PubMed Central

    Tummers, Quirijn R.J.G.; Verbeek, Floris P.R.; Schaafsma, Boudewijn E.; Boonstra, Martin C.; van der Vorst, Joost R.; Liefers, Gerrit-Jan; van de Velde, Cornelis J.H.; Frangioni, John V.; Vahrmeijer, Alexander L.

    2014-01-01

    Background Despite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast-conserving surgery. Based on certain physicochemical similarities between Technetium(99mTc)-sestamibi (MIBI), a SPECT radiodiagnostic with a sensitivity of 83–90% to detect breast cancer preoperatively, and the near-infrared (NIR) fluorophore Methylene Blue (MB), we hypothesized that MB might detect breast cancer intraoperatively using NIR fluorescence imaging. Methods Twenty-four patients with breast cancer, planned for surgical resection, were included. Patients were divided in 2 administration groups, which differed with respect to the timing of MB administration. N = 12 patients per group were administered 1.0 mg/kg MB intravenously either immediately or 3 h before surgery. The mini-FLARE imaging system was used to identify the NIR fluorescent signal during surgery and on post-resected specimens transferred to the pathology department. Results were confirmed by NIR fluorescence microscopy. Results 20/24 (83%) of breast tumors (carcinoma in N=21 and ductal carcinoma in situ in N=3) were identified in the resected specimen using NIR fluorescence imaging. Patients with non-detectable tumors were significantly older. No significant relation to receptor status or tumor grade was seen. Overall tumor-to-background ratio (TBR) was 2.4 ± 0.8. There was no significant difference between TBR and background signal between administration groups. In 2/4 patients with positive resection margins, breast cancer tissue identified in the wound bed during surgery would have changed surgical management. Histology confirmed the concordance of fluorescence signal and tumor tissue. Conclusions This feasibility study demonstrated an overall breast cancer identification rate using MB of 83%, with real-time intraoperative guidance having the potential to alter patient management. PMID

  6. Multi-color imaging of the bacterial nucleoid and division proteins with blue, orange, and near-infrared fluorescent proteins

    PubMed Central

    Wu, Fabai; Van Rijn, Erwin; Van Schie, Bas G. C.; Dekker, Cees

    2015-01-01

    Studies of the spatiotemporal protein dynamics within live bacterial cells impose a strong demand for multi-color imaging. Despite the increasingly large collection of fluorescent protein (FP) variants engineered to date, only a few of these were successfully applied in bacteria. Here, we explore the performance of recently engineered variants with the blue (TagBFP), orange (TagRFP-T, mKO2), and far-red (mKate2) spectral colors by tagging HU, LacI, MinD, and FtsZ for visualizing the nucleoid and the cell division process. We find that, these FPs outperformed previous versions in terms of brightness and photostability at their respective spectral range, both when expressed as cytosolic label and when fused to native proteins. As this indicates that their folding is sufficiently fast, these proteins thus successfully expand the applicable spectra for multi-color imaging in bacteria. A near-infrared protein (eqFP670) is found to be the most red-shifted protein applicable to bacteria so far, with brightness and photostability that are advantageous for cell-body imaging, such as in microfluidic devices. Despite the multiple advantages, we also report the alarming observation that TagBFP directly interacts with TagRFP-T, causing interference of localization patterns between their fusion proteins. Our application of diverse FPs for endogenous tagging provides guidelines for future engineering of fluorescent fusions in bacteria, specifically: (1) The performance of newly developed FPs should be quantified in vivo for their introduction into bacteria; (2) spectral crosstalk and inter-variant interactions between FPs should be carefully examined for multi-color imaging; and (3) successful genomic fusion to the 5′-end of a gene strongly depends on the translational read-through of the inserted coding sequence. PMID:26136737

  7. Real-time out-of-plane artifact subtraction tomosynthesis imaging using prior CT for scanning beam digital x-ray system

    SciTech Connect

    Wu, Meng; Fahrig, Rebecca

    2014-11-01

    Purpose: The scanning beam digital x-ray system (SBDX) is an inverse geometry fluoroscopic system with high dose efficiency and the ability to perform continuous real-time tomosynthesis in multiple planes. This system could be used for image guidance during lung nodule biopsy. However, the reconstructed images suffer from strong out-of-plane artifact due to the small tomographic angle of the system. Methods: The authors propose an out-of-plane artifact subtraction tomosynthesis (OPAST) algorithm that utilizes a prior CT volume to augment the run-time image processing. A blur-and-add (BAA) analytical model, derived from the project-to-backproject physical model, permits the generation of tomosynthesis images that are a good approximation to the shift-and-add (SAA) reconstructed image. A computationally practical algorithm is proposed to simulate images and out-of-plane artifacts from patient-specific prior CT volumes using the BAA model. A 3D image registration algorithm to align the simulated and reconstructed images is described. The accuracy of the BAA analytical model and the OPAST algorithm was evaluated using three lung cancer patients’ CT data. The OPAST and image registration algorithms were also tested with added nonrigid respiratory motions. Results: Image similarity measurements, including the correlation coefficient, mean squared error, and structural similarity index, indicated that the BAA model is very accurate in simulating the SAA images from the prior CT for the SBDX system. The shift-variant effect of the BAA model can be ignored when the shifts between SBDX images and CT volumes are within ±10 mm in the x and y directions. The nodule visibility and depth resolution are improved by subtracting simulated artifacts from the reconstructions. The image registration and OPAST are robust in the presence of added respiratory motions. The dominant artifacts in the subtraction images are caused by the mismatches between the real object and the prior CT

  8. Impacts of Cross-Platform Vicarious Calibration on the Deep Blue Aerosol Retrievals for Moderate Resolution Imaging Spectroradiometer Aboard Terra

    NASA Technical Reports Server (NTRS)

    Jeong, Myeong-Jae; Hsu, N. Christina; Kwiatkowska, Ewa J.; Franz, Bryan A.; Meister, Gerhard; Salustro, Clare E.

    2012-01-01

    The retrieval of aerosol properties from spaceborne sensors requires highly accurate and precise radiometric measurements, thus placing stringent requirements on sensor calibration and characterization. For the Terra/Moderate Resolution Imaging Spedroradiometer (MODIS), the characteristics of the detectors of certain bands, particularly band 8 [(B8); 412 nm], have changed significantly over time, leading to increased calibration uncertainty. In this paper, we explore a possibility of utilizing a cross-calibration method developed for characterizing the Terral MODIS detectors in the ocean bands by the National Aeronautics and Space Administration Ocean Biology Processing Group to improve aerosol retrieval over bright land surfaces. We found that the Terra/MODIS B8 reflectance corrected using the cross calibration method resulted in significant improvements for the retrieved aerosol optical thickness when compared with that from the Multi-angle Imaging Spectroradiometer, Aqua/MODIS, and the Aerosol Robotic Network. The method reported in this paper is implemented for the operational processing of the Terra/MODIS Deep Blue aerosol products.

  9. The coronagraphic Modal Wavefront Sensor: a hybrid focal-plane sensor for the high-contrast imaging of circumstellar environments

    NASA Astrophysics Data System (ADS)

    Wilby, M. J.; Keller, C. U.; Snik, F.; Korkiakoski, V.; Pietrow, A. G. M.

    2017-01-01

    The raw coronagraphic performance of current high-contrast imaging instruments is limited by the presence of a quasi-static speckle (QSS) background, resulting from instrumental Non-Common Path Errors (NCPEs). Rapid development of efficient speckle subtraction techniques in data reduction has enabled final contrasts of up to 10-6 to be obtained, however it remains preferable to eliminate the underlying NCPEs at the source. In this work we introduce the coronagraphic Modal Wavefront Sensor (cMWS), a new wavefront sensor suitable for real-time NCPE correction. This combines the Apodizing Phase Plate (APP) coronagraph with a holographic modal wavefront sensor to provide simultaneous coronagraphic imaging and focal-plane wavefront sensing with the science point-spread function. We first characterise the baseline performance of the cMWS via idealised closed-loop simulations, showing that the sensor is able to successfully recover diffraction-limited coronagraph performance over an effective dynamic range of ±2.5 radians root-mean-square (rms) wavefront error within 2-10 iterations, with performance independent of the specific choice of mode basis. We then present the results of initial on-sky testing at the William Herschel Telescope, which demonstrate that the sensor is capable of NCPE sensing under realistic seeing conditions via the recovery of known static aberrations to an accuracy of 10 nm (0.1 radians) rms error in the presence of a dominant atmospheric speckle foreground. We also find that the sensor is capable of real-time measurement of broadband atmospheric wavefront variance (50% bandwidth, 158 nm rms wavefront error) at a cadence of 50 Hz over an uncorrected telescope sub-aperture. When combined with a suitable closed-loop adaptive optics system, the cMWS holds the potential to deliver an improvement of up to two orders of magnitude over the uncorrected QSS floor. Such a sensor would be eminently suitable for the direct imaging and spectroscopy of

  10. Noninvasive imaging of human skin hemodynamics using a digital red-green-blue camera

    NASA Astrophysics Data System (ADS)

    Nishidate, Izumi; Tanaka, Noriyuki; Kawase, Tatsuya; Maeda, Takaaki; Yuasa, Tomonori; Aizu, Yoshihisa; Yuasa, Tetsuya; Niizeki, Kyuichi

    2011-08-01

    In order to visualize human skin hemodynamics, we investigated a method that is specifically developed for the visualization of concentrations of oxygenated blood, deoxygenated blood, and melanin in skin tissue from digital RGB color images. Images of total blood concentration and oxygen saturation can also be reconstructed from the results of oxygenated and deoxygenated blood. Experiments using tissue-like agar gel phantoms demonstrated the ability of the developed method to quantitatively visualize the transition from an oxygenated blood to a deoxygenated blood in dermis. In vivo imaging of the chromophore concentrations and tissue oxygen saturation in the skin of the human hand are performed for 14 subjects during upper limb occlusion at 50 and 250 mm Hg. The response of the total blood concentration in the skin acquired by this method and forearm volume changes obtained from the conventional strain-gauge plethysmograph were comparable during the upper arm occlusion at pressures of both 50 and 250 mm Hg. The results presented in the present paper indicate the possibility of visualizing the hemodynamics of subsurface skin tissue.

  11. Organic superconductors revisited. STM imaging and DFT based simulations of the bc plane of κ-(BEDT-TTF)2Cu(NCS)2

    NASA Astrophysics Data System (ADS)

    Rohr, C.; Büttner, J. M.; Palitschka, F. A.; Kushch, N. D.; Kartsovnik, M. V.; Biberacher, W.; Gross, R.; Hermann, B. A.

    2009-05-01

    The surfaces of a ten years aged crystal and a freshly prepared κ-(BEDT-TTF)2Cu(NCS)2 crystal were compared by scanning tunneling microscopy (STM). The molecularly-resolved STM images of the bc plane of the crystals agree with each other and with the electronic contrast obtained by new density functional theory (DFT) based simulations. Even after ten years STM images of the molecular stacking of BEDT-TTF display a variation in brightness at the positions of different molecules. We attribute this symmetry breaking concerning the brightness in the STM images of the otherwise equivalent BEDT-TTF dimers to the electronic states of a relaxed surface.

  12. Comparison of the diagnostic ability of blue laser imaging magnification versus pit pattern analysis for colorectal polyps

    PubMed Central

    Nakano, Arihiro; Hirooka, Yoshiki; Yamamura, Takeshi; Watanabe, Osamu; Nakamura, Masanao; Funasaka, Kohei; Ohno, Eizaburo; Kawashima, Hiroki; Miyahara, Ryoji; Goto, Hidemi

    2017-01-01

    Background and study aims There have been few evaluations of the diagnostic ability of new narrow band light observation blue laser imaging (BLI). The present prospective study compared the diagnostic ability of BLI magnification and pit pattern analysis for colorectal polyps. Patients and methods We collected lesions prospectively, and the analysis of images was made by two endoscopists, retrospectively. A total of 799 colorectal polyps were examined by BLI magnification and pit pattern analysis at Nagoya University Hospital. The Hiroshima narrow-band imaging classification was used for BLI. Differentiation of neoplastic from non-neoplastic lesions and diagnosis of deeply invasive submucosal cancer (dSM) were compared between BLI magnification and pit pattern analysis. Type C2 in the Hiroshima classification was evaluated separately, because application of this category as an index of the depth of cancer invasion was considered difficult. Results We analyzed 748 colorectal polyps, excluding 51 polyps that were inflammatory polyps, sessile serrated adenoma/polyps, serrated adenomas, advanced colorectal cancers, or other lesions. The accuracy of differential diagnosis between neoplastic and non-neoplastic lesions was 98.4 % using BLI magnification and 98.7 % with pit pattern analysis. In addition, the diagnostic accuracy of BLI magnification and pit pattern analysis for dSM for cancer was 89.5 % and 92.1 %, respectively. When type C2 lesions were excluded, the diagnostic accuracy of BLI for dSM was 95.9 %. The 18 type C2 lesions comprised 1 adenoma, 9 intramucosal or slightly invasive submucosal cancers, and 8 dSM. Pit pattern analysis allowed accurate diagnosis of the depth of invasion in 13 lesions (72.2 %). Conclusions Most colorectal polyps could be diagnosed accurately by BLI magnification without pit pattern analysis, but we should add pit pattern analysis for type C2 lesions in the Hiroshima classification. PMID:28367494

  13. [Use of blue and green systems of image visualization in roentgenology].

    PubMed

    Riuduger, Iu G

    2004-01-01

    The main peculiarities of two image visualization systems related with the specificity of intensifying screens and of radiographic films in each of them are discussed. Specific features of kinetic development of modern orthochromatic general-purpose radiographic films were studied versus those of the traditional films; differences related with radiation hardness of some of the intensifying screen manufactured in Russia were investigated. Some practical advice was suggested on the basis of a conducted analysis of the "green" system specificity; such advice provides for reorienting the X-ray examination room, in Russia, for gadolinium screens and modern radiography films.

  14. [Maternity blues].

    PubMed

    Gonidakis, F

    2007-04-01

    Maternity blues is a transient change of mood that occurs mainly between the 1st and 10th day of puerpartum and is characterized by bursts of tears, mild depressive mood, anxiety and liability of mood. The frequency of maternity blues varies in different studies form 4% to 80%. A number of biological and psychosocial parameters have been studied in order to determine their correlation with maternity blues. The most well studied biological parameters are progesterone and cortizol although their relation with maternity blues has not yet been clearly defined. Stress and the emotional state of the woman during pregnancy as well as history of mood disorders or maternity blues in a previous birth are the psychosocial parameters that are more likely to correlate with the occurrence of maternity blues. Most of the authors suggest that information on maternity blues and reassurance of the woman are the best way to deal with maternity blues both on preventive and therapeutical basis.

  15. In vivo multispectral imaging of the absorption and scattering properties of exposed brain using a digital red-green-blue camera

    NASA Astrophysics Data System (ADS)

    Yoshida, Keiichiro; Ishizuka, Tomohiro; Mizushima, Chiharu; Nishidate, Izumi; Kawauchi, Satoko; Sato, Shunichi; Sato, Manabu

    2015-04-01

    To evaluate multi-spectral images of the absorption and scattering properties in the cerebral cortex of rat brain, we investigated spectral reflectance images estimated by the Wiener estimation method using a digital red-green-blue camera. A Monte Carlo simulation-based multiple regression analysis for the corresponding spectral absorbance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) was then used to specify the absorption and scattering parameters. The spectral images of absorption and reduced scattering coefficients were reconstructed from the absorption and scattering parameters. We performed in vivo experiments on exposed rat brain to confirm the feasibility of this method. The estimated images of the absorption coefficients were dominated by hemoglobin spectra. The estimated images of the reduced scattering coefficients had a broad scattering spectrum, exhibiting a larger magnitude at shorter wavelengths, corresponding to the typical spectrum of brain tissue published in the literature.

  16. Digital image correlation applied to the calculation of the out-of-plane deformation induced by the formation of roll waves in a non-Newtonian fluid

    NASA Astrophysics Data System (ADS)

    Aranda, Alfredo; Amigo, Nicolás; Ihle, Christian; Tamburrino, Aldo

    2016-06-01

    A method based on digital image correlation (DIC) is implemented for measuring the height of the roll waves developed in a non-Newtonian fluid flowing on an inclined channel. A projector and a high-resolution digital camera, placed vertically above the fluid surface, are used to project and record a random speckle pattern located on the free liquid surface, where the pattern is deformed due to the developed roll waves. According to the experimental geometry, the height of the roll waves associated to the out-of-plane deformation of the dots is obtained through a quantitative relationship between the experimental parameters and the in-plane displacement field in the flow direction. In terms of this, the out-of-plane deformation is found using a DIC criterion based on the speckle comparison between a reference image without the deformed pattern and an image with a deformed pattern. The maximum height of the roll waves computed with this technique is compared with the height measured using a lateral camera, with both results differing by <10% over the set of experimental instances.

  17. Method and phantom to study combined effects of in-plane (x,y) and z-axis resolution for 3D CT imaging.

    PubMed

    Goodenough, David; Levy, Josh; Kristinsson, Smari; Fredriksson, Jesper; Olafsdottir, Hildur; Healy, Austin

    2016-09-01

    Increasingly, the advent of multislice CT scanners, volume CT scanners, and total body spiral acquisition modes has led to the use of Multi Planar Reconstruction and 3D datasets. In considering 3D resolution properties of a CT system it is important to note that both the in-plane (x,y) and z-axis (slice thickness) influence the visualization and detection of objects within the scanned volume. This study investigates ways to consider both the in-plane resolution and the z-axis resolution in a single phantom wherein analytic or visualized analysis can yield information on these combined effects. A new phantom called the "Wave Phantom" is developed that can be used to sample the 3D resolution properties of a CT image, including in-plane (x,y) and z-axis information. The key development in this Wave Phantom is the incorporation of a z-axis aspect of a more traditional step (bar) resolution gauge phantom. The phantom can be examined visually wherein a cutoff level may be seen; and/or the analytic analysis of the various characteristics of the waveform profile by including amplitude, frequency, and slope (rate of climb) of the peaks, can be extracted from the Wave Pattern using mathematical analysis such as the Fourier transform. The combined effect of changes in in-plane resolution and z-axis (thickness), are shown, as well as the effect of changes in either in-plane resolution, or z-axis thickness. Examples of visual images of the Wave pattern as well as the analytic characteristics of the various harmonics of a periodic Wave pattern resulting from changes in resolution filter and/or slice thickness, and position in the field of view are shown. The Wave Phantom offers a promising way to investigate 3D resolution results from combined effect of in-plane (x-y) and z-axis resolution as contrasted to the use of simple 2D resolution gauges that need to be used with separate measures of z-axis dependency, such as angled ramps. It offers both a visual pattern as well as a

  18. Method and phantom to study combined effects of in-plane (x,y) and z-axis resolution for 3D CT imaging.

    PubMed

    Goodenough, David; Levy, Josh; Kristinsson, Smari; Fredriksson, Jesper; Olafsdottir, Hildur; Healy, Austin

    2016-09-08

    Increasingly, the advent of multislice CT scanners, volume CT scanners, and total body spiral acquisition modes has led to the use of Multi Planar Reconstruction and 3D datasets. In considering 3D resolution properties of a CT system it is important to note that both the in-plane (x,y) and z-axis (slice thickness) influence the visual-ization and detection of objects within the scanned volume. This study investigates ways to consider both the in-plane resolution and the z-axis resolution in a single phantom wherein analytic or visualized analysis can yield information on these combined effects. A new phantom called the "Wave Phantom" is developed that can be used to sample the 3D resolution properties of a CT image, including in-plane (x,y) and z-axis information. The key development in this Wave Phantom is the incorporation of a z-axis aspect of a more traditional step (bar) resolution gauge phantom. The phantom can be examined visually wherein a cutoff level may be seen; and/or the analytic analysis of the various characteristics of the waveform profile by including amplitude, frequency, and slope (rate of climb) of the peaks, can be extracted from the Wave Pattern using mathematical analysis such as the Fourier transform. The combined effect of changes in in-plane resolution and z-axis (thickness), are shown, as well as the effect of changes in either in-plane resolu-tion, or z-axis thickness. Examples of visual images of the Wave pattern as well as the analytic characteristics of the various harmonics of a periodic Wave pattern resulting from changes in resolution filter and/or slice thickness, and position in the field of view are shown. The Wave Phantom offers a promising way to investigate 3D resolution results from combined effect of in-plane (x-y) and z-axis resolution as contrasted to the use of simple 2D resolution gauges that need to be used with separate measures of z-axis dependency, such as angled ramps. It offers both a visual pattern as well as a

  19. SNAP focal plane

    SciTech Connect

    Lampton, Michael L.; Kim, A.; Akerlof, C.W.; Aldering, G.; Amanullah, R.; Astier, P.; Barrelet, E.; Bebek, C.; Bergstrom, L.; Berkovitz, J.; Bernstein, G.; Bester, M.; Bonissent, A.; Bower, C.; Carithers Jr., W.C.; Commins, E.D.; Day, C.; Deustua, S.E.; DiGennaro,R.; Ealet, A.; Ellis, R.S.; Eriksson, M.; Fruchter, A.; Genat, J.-F.; Goldhaber, G.; Goobar, A.; Groom, D.; Harris, S.E.; Harvey, P.R.; Heetderks, H.D.; Holland, S.E.; Huterer, D.; Karcher, A.; Kolbe, W.; Krieger, B.; Lafever, R.; Lamoureux, J.; Levi, M.E.; Levin, D.S.; Linder,E.V.; Loken, S.C.; Malina, R.; Massey, R.; McKay, T.; McKee, S.P.; Miquel, R.; Mortsell, E.; Mostek, N.; Mufson, S.; Musser, J.; Nugent, P.; Oluseyi, H.; Pain, R.; Palaio, N.; Pankow, D.; Perlmutter, S.; Pratt, R.; Prieto, E.; Refregier, A.; Rhodes, J.; Robinson, K.; Roe, N.; Sholl, M.; Schubnell, M.; Smadja, G.; Smoot, G.; Spadafora, A.; Tarle, G.; Tomasch,A.; von der Lippe, H.; Vincent, R.; Walder, J.-P.; Wang, G.

    2002-07-29

    The proposed SuperNova/Acceleration Probe (SNAP) mission will have a two-meter class telescope delivering diffraction-limited images to an instrumented 0.7 square-degree field sensitive in the visible and near-infrared wavelength regime. We describe the requirements for the instrument suite and the evolution of the focal plane design to the present concept in which all the instrumentation--visible and near-infrared imagers, spectrograph, and star guiders--share one common focal plane.

  20. Dyed red, green, and blue photoresist for manufacture of high-resolution color filter arrays for image sensors

    NASA Astrophysics Data System (ADS)

    Guerrero, Douglas J.; DiMenna, William; Flaim, Tony D.; Mercado, Ramil; Sun, Sam

    2003-05-01

    We are developing a set of dyed red, green, and blue color filter coatings for the fabrication of high resolution CCD and CMOS image sensor arrays. The resists contain photosensitive polymer binders and various curing agents, soluble organic dyes, and solvents. The new dyed photoresists are sensitive to i-line radiation, primarily at 365 nm, and are negative-working, requiring less than 500 mJ of exposure energy for patterning. The coatings are developed in standard Tetramethylammonium Hydroxide (TMAH) developers. Many dyes were examined in order to achieve the desired spectral properties as well as the meet the solvent solubility and thermal stability requirements. Computer modeling was utilized to determine the correct proportions of dye(s) in each resist, after which the modeling results were verified by actual formulation and testing. Thermal stability of the dyes was determined using isothermal. Thermogravimetric Analysis (TGA) at 200°C for 30 minutes. The dyes were evaluated in both traditional (free radical) and novel polymer systems to see if adequate sensitivity, resolution, and feature quality could be obtained. The studies showed that traditional free radical-based photochemistries are marginal at best for high resolution (1-2 micron) applications. To overcome this limitation, a new polymer system having photodimerizable functional units and acid functional groups was developed to impart photosensitivity and developer solubility, respectively. This system, which does not use free radical-initiated photopolymerization as a mechanism for patterning, shows low exposure dose requirements and is capable of resolving features less than 2 micron in size.

  1. Blue Note

    ScienceCinema

    Murray Gibson

    2016-07-12

    Argonne's Murray Gibson is a physicist whose life's work includes finding patterns among atoms. The love of distinguishing patterns also drives Gibson as a musician and Blues enthusiast."Blue" notes are very harmonic notes that are missing from the equal temperament scale.The techniques of piano blues and jazz represent the melding of African and Western music into something totally new and exciting.

  2. Blue Note

    SciTech Connect

    Murray Gibson

    2007-04-27

    Argonne's Murray Gibson is a physicist whose life's work includes finding patterns among atoms. The love of distinguishing patterns also drives Gibson as a musician and Blues enthusiast."Blue" notes are very harmonic notes that are missing from the equal temperament scale.The techniques of piano blues and jazz represent the melding of African and Western music into something totally new and exciting.

  3. Imaging, photophysical properties and DFT calculations of manganese blue (barium manganate(VI) sulphate)--a modern pigment.

    PubMed

    Accorsi, Gianluca; Verri, Giovanni; Acocella, Angela; Zerbetto, Francesco; Lerario, Giovanni; Gigli, Giuseppe; Saunders, David; Billinge, Rachel

    2014-12-18

    Manganese blue is a synthetic barium manganate(VI) sulphate compound that was produced from 1935 to the 1990s and was used both as a blue pigment in works of art and by conservators in the restoration of paintings. The photophysical properties of the compound are described as well as the setup needed to record the spatial distribution of the pigment in works of art.

  4. Plane Jane(s).

    ERIC Educational Resources Information Center

    Greenman, Geri

    2001-01-01

    Describes an assignment that was used in an advanced drawing class in which the students created self-portraits, breaking up their images using planes and angles to suggest their bone structure. Explains that the students also had to include three realistic portions in their drawings. (CMK)

  5. Practical utility of the blue spectral region

    NASA Technical Reports Server (NTRS)

    Ross, D. S.

    1972-01-01

    Some aspects of multispectral photography in the blue region are discussed briefly, and sample images are submitted to demonstrate the potential utility of the blue multispectral record for oceanography.

  6. Methylene Blue Assay for Estimation of Regenerative Re-Epithelialization In Vivo.

    PubMed

    Milyavsky, Maresha; Dickie, Renee

    2017-02-01

    The rapidity with which epithelial cells cover a wound surface helps determine whether scarring or scar-less healing results. As methylene blue is a vital dye that is absorbed by damaged tissue but not undamaged epidermis, it can be used to assess wound closure. We sought to develop a quantitative methylene blue exclusion assay to estimate the timeframe for re-epithelialization in regenerating appendages in zebrafish and axolotls, two classic model systems of regeneration. Following application of methylene blue to the amputation plane and extensive washing, the regenerating tail was imaged in vivo until staining was no longer visible. The percent area of the amputation plane positive for methylene blue, representing the area of the amputation plane not yet re-epithelialized, was measured for each time point. The loss of methylene blue occurred rapidly, within ~2.5 h in larval and juvenile axolotls and <1 h in adult zebrafish, consistent with high rates of re-epithelialization in these models of regeneration. The assay allows simple, rapid estimation of the time course for regenerative re-epithelialization without affecting subsequent regenerative ability. This technique will permit comparison of re-epithelialization across different strains and stages, as well as under the influence of various pharmacological inhibitors that affect regeneration.

  7. Composite x-ray image assembly for large-field digital mammography with one- and two-dimensional positioning of a focal plane array

    NASA Technical Reports Server (NTRS)

    Halama, G.; McAdoo, J.; Liu, H.

    1998-01-01

    To demonstrate the feasibility of a novel large-field digital mammography technique, a 1024 x 1024 pixel Loral charge-coupled device (CCD) focal plane array (FPA) was positioned in a mammographic field with one- and two-dimensional scan sequences to obtain 950 x 1800 pixel and 3600 x 3600 pixel composite images, respectively. These experiments verify that precise positioning of FPAs produced seamless composites and that the CCD mosaic concept has potential for high-resolution, large-field imaging. The proposed CCD mosaic concept resembles a checkerboard pattern with spacing left between the CCDs for the driver and readout electronics. To obtain a complete x-ray image, the mosaic must be repositioned four times, with an x-ray exposure at each position. To reduce the patient dose, a lead shield with appropriately patterned holes is placed between the x-ray source and the patient. The high-precision motorized translation stages and the fiber-coupled-scintillating-screen-CCD sensor assembly were placed in the position usually occupied by the film cassette. Because of the high mechanical precision, seamless composites were constructed from the subimages. This paper discusses the positioning, image alignment procedure, and composite image results. The paper only addresses the formation of a seamless composite image from subimages and will not consider the effects of the lead shield, multiple CCDs, or the speed of motion.

  8. Pixel color clustering of multi-temporally acquired digital photographs of a rice canopy by luminosity-normalization and pseudo-red-green-blue color imaging.

    PubMed

    Doi, Ryoichi; Arif, Chusnul; Setiawan, Budi Indra; Mizoguchi, Masaru

    2014-01-01

    Red-green-blue (RGB) channels of RGB digital photographs were loaded with luminosity-adjusted R, G, and completely white grayscale images, respectively (RGwhtB method), or R, G, and R + G (RGB yellow) grayscale images, respectively (RGrgbyB method), to adjust the brightness of the entire area of multi-temporally acquired color digital photographs of a rice canopy. From the RGwhtB or RGrgbyB pseudocolor image, cyan, magenta, CMYK yellow, black, L*, a*, and b* grayscale images were prepared. Using these grayscale images and R, G, and RGB yellow grayscale images, the luminosity-adjusted pixels of the canopy photographs were statistically clustered. With the RGrgbyB and the RGwhtB methods, seven and five major color clusters were given, respectively. The RGrgbyB method showed clear differences among three rice growth stages, and the vegetative stage was further divided into two substages. The RGwhtB method could not clearly discriminate between the second vegetative and midseason stages. The relative advantages of the RGrgbyB method were attributed to the R, G, B, magenta, yellow, L*, and a* grayscale images that contained richer information to show the colorimetrical differences among objects than those of the RGwhtB method. The comparison of rice canopy colors at different time points was enabled by the pseudocolor imaging method.

  9. Pluto’s Blue Haze

    NASA Video Gallery

    The sky on Pluto is blue! Kind of. This is Pluto in an Minute. So it’s not exactly the case that the sky on Pluto is blue, rather, what the New Horizons science team has found in recent images do...

  10. Blue Laser.

    DTIC Science & Technology

    1985-12-01

    HOLLOW CATHODE LASER FABRICATION 13 4. EXPERIENCE WITH THE BLUE LASER 18 4.1 Operational and Processing Experience 18 4.2 Performance Testing 20 5...34 -. - . •. SECTION 3 BLUE HOLLOW CATHODE LASER FABRICATION This section presents an overview of the steps taken in creating a HCL. There is...to the laser assembly. These steps can actually be considered as the final steps in laser fabrication because some of them involve adding various

  11. Characterization and comparison of three microfabrication methods to generate out-of-plane microvortices for single cell rotation and 3D imaging

    NASA Astrophysics Data System (ADS)

    Shetty, Rishabh M.; Myers, Jakrey R.; Sreenivasulu, Manoj; Teller, Wacey; Vela, Juan; Houkal, Jeff; Chao, Shih-Hui; Johnson, Roger H.; Kelbauskas, Laimonas; Wang, Hong; Meldrum, Deirdre R.

    2017-01-01

    This paper presents three different microfabrication technologies for manufacturing out-of-plane, flat-bottomed, undercut trapezoidal structures for generating a fluidic microscale vortex (microvortex). The first method is based on anisotropic silicon etching and a ‘sandwich’ UV polymer casting assembly; the second method uses a backside diffuser photolithography technique; and the third method features a tilted backside photolithography technique. We discuss the advantages, limitations, and utility of each technique. We further demonstrate that the microvortex generated in the resultant undercut trapezoidal structures can be used to rotate biological microparticles, e.g. single, live cells for multiperspective, high resolution 3D imaging using computed tomography, and angularly resolved confocal imaging.

  12. Method of holographic imaging of inhomogeneities in the density of a substance of a semitransparent medium using plane-parallel laser radiation

    NASA Astrophysics Data System (ADS)

    Volosyuk, V. K.; Zhyla, S. S.; Kravchenko, V. F.; Morozov, A. V.; Pustovoit, V. I.; Hunziker, P.

    2016-12-01

    A new method for coherent superbroadband sounding of a semitransparent substance with the aim to construct a three-dimensional image of its inner structure is proposed and justified. The method is based on irradiating the studied medium with a plane ray, spectral analysis by the two-dimensional Fresnel-Fourier transform, interferometric comparison of spatial spectra of scattered and reference signals, and holographic construction of a series of two-dimensional images of the medium followed by tomographic reconstruction of its three-dimensional image. The stochastic image model has been justified in a coherent approach for the first time both in the region of high frequencies and after the transfer of the radiation spectrum to the lowfrequency range of spatial frequencies to the surface of the radiation recorder. The spectral correlation characteristics of the speckle structure of the reconstructed image have been studied. Calculations and investigations of the spread function of the structure implementing the proposed method have been carried out.

  13. Voyager 1 'Blue Movie'

    NASA Technical Reports Server (NTRS)

    2000-01-01

    This is the original Voyager 'Blue Movie' (so named because it was built from Blue filter images). It records the approach of Voyager 1 during a period of over 60 Jupiter days. Notice the difference in speed and direction of the various zones of the atmosphere. The interaction of the atmospheric clouds and storms shows how dynamic the Jovian atmosphere is.

    As Voyager 1 approached Jupiter in 1979, it took images of the planet at regular intervals. This sequence is made from 66 images taken once every Jupiter rotation period (about 10 hours). This time-lapse movie uses images taken every time Jupiter longitude 68W passed under the spacecraft. These images were acquired in the Blue filter from Jan. 6 to Feb. 3 1979. The spacecraft flew from 58 million kilometers to 31 million kilometers from Jupiter during that time.

    This time-lapse movie was produced at JPL by the Image Processing Laboratory in 1979.

  14. The Q/U Imaging Experiment: Polarization Measurements of the Galactic Plane at 43 and 95 GHz

    NASA Astrophysics Data System (ADS)

    Ruud, T. M.; Fuskeland, U.; Wehus, I. K.; Vidal, M.; Araujo, D.; Bischoff, C.; Buder, I.; Chinone, Y.; Cleary, K.; Dumoulin, R. N.; Kusaka, A.; Monsalve, R.; Næss, S. K.; Newburgh, L. B.; Reeves, R. A.; Zwart, J. T. L.; Bronfman, L.; Davies, R. D.; Davis, R.; Dickinson, C.; Eriksen, H. K.; Gaier, T.; Gundersen, J. O.; Hasegawa, M.; Hazumi, M.; Huffenberger, K. M.; Jones, M. E.; Lawrence, C. R.; Leitch, E. M.; Limon, M.; Miller, A. D.; Pearson, T. J.; Piccirillo, L.; Radford, S. J. E.; Readhead, A. C. S.; Samtleben, D.; Seiffert, M.; Shepherd, M. C.; Staggs, S. T.; Tajima, O.; Thompson, K. L.; QUIET Collaboration

    2015-10-01

    We present polarization observations of two Galactic plane fields centered on Galactic coordinates (l, b) = (0°, 0°) and (329°, 0°) at both Q (43 GHz) and W bands (95 GHz), covering between 301 and 539 square degrees depending on frequency and field. These measurements were made with the QUIET instrument between 2008 October and 2010 December, and include a total of 1263 hr of observations. The resulting maps represent the deepest large-area Galactic polarization observations published to date at the relevant frequencies with instrumental rms noise varying between 1.8 and 2.8 μK deg, 2.3-6 times deeper than corresponding WMAP and Planck maps. The angular resolution is 27.‧3 and 12.‧8 FWHM at Q and W bands, respectively. We find excellent agreement between the QUIET and WMAP maps over the entire fields, and no compelling evidence for significant residual instrumental systematic errors in either experiment, whereas the Planck 44 GHz map deviates from these in a manner consistent with reported systematic uncertainties for this channel. We combine QUIET and WMAP data to compute inverse-variance-weighted average maps, effectively retaining small angular scales from QUIET and large angular scales from WMAP. From these combined maps, we derive constraints on several important astrophysical quantities, including a robust detection of polarized synchrotron spectral index steepening of ≈0.2 off the plane, as well as the Faraday rotation measure toward the Galactic center (RM = -4000 ± 200 rad m-2), all of which are consistent with previously published results. Both the raw QUIET and the co-added QUIET+WMAP maps are made publicly available together with all necessary ancillary information.

  15. Least squares restoration of multichannel images

    NASA Technical Reports Server (NTRS)

    Galatsanos, Nikolas P.; Katsaggelos, Aggelos K.; Chin, Roland T.; Hillery, Allen D.

    1991-01-01

    Multichannel restoration using both within- and between-channel deterministic information is considered. A multichannel image is a set of image planes that exhibit cross-plane similarity. Existing optimal restoration filters for single-plane images yield suboptimal results when applied to multichannel images, since between-channel information is not utilized. Multichannel least squares restoration filters are developed using the set theoretic and the constrained optimization approaches. A geometric interpretation of the estimates of both filters is given. Color images (three-channel imagery with red, green, and blue components) are considered. Constraints that capture the within- and between-channel properties of color images are developed. Issues associated with the computation of the two estimates are addressed. A spatially adaptive, multichannel least squares filter that utilizes local within- and between-channel image properties is proposed. Experiments using color images are described.

  16. 4D in-vivo ultrafast ultrasound imaging using a row-column addressed matrix and coherently-compounded orthogonal plane waves.

    PubMed

    Flesch, Martin; Pernot, Mathieu; Provost, Jean; Ferin, Guillaume; Nguyen-Dinh, An; Tanter, Mickael; Deffieux, Thomas

    2017-03-01

    4D ultrafast ultrasound imaging was recently shown using a 2D matrix (i.e., fully populated) connected to a 1024-channel ultrafast ultrasound scanner. In this study, we investigate the Row-Column Addressing (RCA) matrix approach, which allows a reduction of independent channels from N x N to N + N, with a dedicated beamforming strategy for ultrafast ultrasound imaging based on the coherent compounding of Orthogonal Plane Wave (OPW). OPW is based on coherent compounding of plane wave transmissions in one direction with receive beamforming along the orthogonal direction and its orthogonal companion sequence. Such coherent recombination of complementary orthogonal sequences leads to virtual transmit focusing in both directions which results into a final isotropic Point Spread Function (PSF). In this study, a 32 x 32 2D matrix array probe (1024 channels), centered at 5 MHz was considered. An RCA array, of same footprint with 32 + 32 elements (64 channels), was emulated by summing the elements either along a line or a column in software prior to beamforming. This approach allowed for the direct comparison of the 32 + 32 RCA scheme to the optimal fully sampled 32 x 32 2D matrix configuration, which served as the gold standard. This approach was first studied through PSF simulations and then validated experimentally on a phantom consisting of anechoic cysts and echogenic wires. The Contrast-to-Noise Ratio (CNR) and the lateral resolution of the RCA approach were found to be approximately equal to half (in decibel) and twice the values, respectively, obtained when using the 2D matrix approach. Results in a Doppler phantom and the human humeral artery in vivo confirmed that OPW compound imaging using emulated RCA matrix can achieve a power Doppler with sufficient contrast to recover the vein shape and provides an accurate Doppler spectrum.

  17. On Seeing Reddish Green and Yellowish Blue.

    ERIC Educational Resources Information Center

    Crane, Hewitt D.; Piantanida, Thomas P.

    1983-01-01

    Stabilization of the retinal image of the boundary between a pair of red/green or yellow/blue stripes, but not their outer edges, results in the entire region being perceived simultaneously as both red/green or yellow/blue. This suggests that the percepts of reddish-green/yellowish-blue apparently are possible in corticocortical color vision…

  18. Integrating Spray Plane-Based Remote Sensing and Rapid Image Processing with Variable-Rate Aerial Application.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A remote sensing and variable rate application system was configured for agricultural aircraft. This combination system has the potential of providing a completely integrated solution for all aspects of aerial site-specific application and includes remote sensing, image processing and georegistratio...

  19. Low-radio-frequency eclipses of the redback pulsar J2215+5135 observed in the image plane with LOFAR.

    PubMed

    Broderick, J W; Fender, R P; Breton, R P; Stewart, A J; Rowlinson, A; Swinbank, J D; Hessels, J W T; Staley, T D; van der Horst, A J; Bell, M E; Carbone, D; Cendes, Y; Corbel, S; Eislöffel, J; Falcke, H; Grießmeier, J-M; Hassall, T E; Jonker, P; Kramer, M; Kuniyoshi, M; Law, C J; Markoff, S; Molenaar, G J; Pietka, M; Scheers, L H A; Serylak, M; Stappers, B W; Ter Veen, S; van Leeuwen, J; Wijers, R A M J; Wijnands, R; Wise, M W; Zarka, P

    2016-07-01

    The eclipses of certain types of binary millisecond pulsars (i.e. 'black widows' and 'redbacks') are often studied using high-time-resolution, 'beamformed' radio observations. However, they may also be detected in images generated from interferometric data. As part of a larger imaging project to characterize the variable and transient sky at radio frequencies <200 MHz, we have blindly detected the redback system PSR J2215+5135 as a variable source of interest with the Low-Frequency Array (LOFAR). Using observations with cadences of two weeks - six months, we find preliminary evidence that the eclipse duration is frequency dependent (∝ν(-0.4)), such that the pulsar is eclipsed for longer at lower frequencies, in broad agreement with beamformed studies of other similar sources. Furthermore, the detection of the eclipses in imaging data suggests an eclipsing medium that absorbs the pulsed emission, rather than scattering it. Our study is also a demonstration of the prospects of finding pulsars in wide-field imaging surveys with the current generation of low-frequency radio telescopes.

  20. Single-wavelength-controlled in situ dynamic super-resolution fluorescence imaging for block copolymer nanostructures via blue-light-switchable FRAP.

    PubMed

    Gong, Wen-Liang; Yan, Jie; Zhao, Ling-Xi; Li, Chong; Huang, Zhen-Li; Tang, Ben Zhong; Zhu, Ming-Qiang

    2016-11-02

    Photoswitchable fluorophores are promising in single-molecule optical devices and super-resolution fluorescence imaging, especially in single-molecule photo-activated localization microscopy (PALM) or stochastic optical reconstruction microscopy (STORM). However, the scarcity of current photoswitchable fluorophores stimulates researchers to develop complicated optical systems and processing software, in accordance with the limited photoswitchable fluorescent proteins and organic fluorophores. Previous efforts to develop synthetic photoswitchable fluorophores have exhibited their promising potential in super-resolution fluorescence imaging. Here, we have designed and synthesized a fluorescence molecular switch with reversible green emission, a napthalimide-hexaarylbiimidazole conjugate (NI-N-HABI), which exhibits strong fluorescence in the emissive state, with fast thermal fading of the photochromism and spontaneous fluorescence recovery after photobleaching (FRAP) induced by blue-light. The photoswitchable fluorophore enables the red-edge wavelength of the optical response to red-shift from the initial near-UV region at less than 400 nm, to 500 nm. The relatively fast fading speed of NI-N-HABI and its sensitivity to longer blue-light irradiation (400-500 nm) have allowed simplification of the optical microscopic system from a two-wavelength laser source to a single-wavelength laser. We applied NI-N-HABI in single-wavelength-controlled in situ dynamic super-resolution fluorescence imaging for the self-assembly and solvent annealing of amphiphilic block polymers, with 50 nm of optical resolution. Single-wavelength-controlled dynamic super-resolution fluorescence imaging facilitates nanoscale optical visualization for the dynamic physical and chemical fluctuation processes of stimuli-responsive nanostructures.

  1. Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium

    SciTech Connect

    Quaroni, Luca; Zlateva, Theodora; Sarafimov, Blagoj; Kreuzer, Helen W.; Wehbe, Katia; Hegg, Eric L.; Cinque, Gianfelice

    2014-03-26

    We tested the viability of using synchrotron based infrared imaging to study biochemical processes inside living cells. As a model system, we studied fibroblast cells exposed to a medium highly enriched with D2O. We could show that the experimental technique allows us to reproduce at the cellular level measurements that are normally performed on purified biological molecules. We can obtain information about lipid conformation and distribution, kinetics of hydrogen/deuterium exchange, and the formation of concentration gradients of H and O isotopes in water that are associated with cell metabolism. The implementation of the full field technique in a sequential imaging format gives a description of cellular biochemistry and biophysics that contains both spatial and temporal information.

  2. Blue gods, blue oil, and blue people.

    PubMed

    Fairbanks, V F

    1994-09-01

    Studies of the composition of coal tar, which began in Prussia in 1834, profoundly affected the economies of Germany, Great Britain, India, and the rest of the world, as well as medicine and surgery. Such effects include the collapse of the profits of the British indigo monopoly, the growth in economic power of Germany based on coal tar chemistry, and an economic crisis in India that led to more humane tax laws and, ultimately, the independence of India and the end of the British Empire. Additional consequences were the development of antiseptic surgery and the synthesis of a wide variety of useful drugs that have eradicated infections and alleviated pain. Many of these drugs, particularly the commonly used analgesics, sulfonamides, sulfones, and local anesthetics, are derivatives of aniline, originally called "blue oil" or "kyanol." Some of these aniline derivatives, however, have also caused aplastic anemia, agranulocytosis, and methemoglobinemia (that is, "blue people"). Exposure to aniline drugs, particularly when two or three aniline drugs are taken concurrently, seems to be the commonest cause of methemoglobinemia today.

  3. Characterizing the transplanar and in-plane water transport of textiles with gravimetric and image analysis technique: Spontaneous Uptake Water Transport Tester

    PubMed Central

    Tang, K. P. M.; Wu, Y. S.; Chau, K. H.; Kan, C. W.; Fan, J. T.

    2015-01-01

    Water absorption and transport property of textiles is important since it affects wear comfort, efficiency of treatment and functionality of product. This paper introduces an accurate and reliable measurement tester, which is based on gravimetric and image analysis technique, for characterising the transplanar and in-plane wicking property of fabrics. The uniqueness of this instrument is that it is able to directly measure the water absorption amount in real-time, monitor the direction of water transport and estimate the amount of water left on skin when sweating. Throughout the experiment, water supply is continuous which simulates profuse sweating. Testing automation could even minimise variation caused by subjective manipulation, thus enhancing testing accuracy. This instrument is versatile in terms of the fabrics could be tested. A series of shirting fabrics made by different fabric structure and yarn were investigated and the results show that the proposed method has high sensitivity in differentiating fabrics with varying geometrical differences. Fabrics with known hydrophobicity were additionally tested to examine the sensitivity of the instrument. This instrument also demonstrates the flexibility to test on high performance moisture management fabrics and these fabrics were found to have excellent transplanar and in-plane wicking properties. PMID:25875329

  4. Autofocus technique for three-dimensional imaging, direct-detection laser radar using Geiger-mode avalanche photodiode focal-plane array.

    PubMed

    Oh, Min Seok; Kong, Hong Jin; Kim, Tae Hoon; Jo, Sung Eun

    2010-12-15

    An autofocus technique is proposed for a three-dimensional imaging, direct-detection laser radar system that uses a Geiger-mode avalanche photodiode focal plane array (GmAPD-FPA). This technique is implemented by pointing laser pulses on a target of interest and observing its scattered photon distribution on a GmAPD-FPA. Measuring the standard deviation of the photon distribution on a GmAPD-FPA enables the best focus condition to be found. The feasibility of this technique is demonstrated experimentally by employing a 1 × 8 pixel GmAPD-FPA. It is shown that the spatial resolution improves when the GmAPD-FPA is located in the best focus position found by the autofocus technique.

  5. Sodium cholate-templated blue light-emitting Ag subnanoclusters: in vivo toxicity and imaging in zebrafish embryos.

    PubMed

    Chandirasekar, Shanmugam; Chandrasekaran, Chandramouli; Muthukumarasamyvel, Thangavel; Sudhandiran, Ganapasam; Rajendiran, Nagappan

    2015-01-28

    We report a novel green chemical approach for the synthesis of blue light-emitting and water-soluble Ag subnanoclusters, using sodium cholate (NaC) as a template at a concentration higher than the critical micelle concentration (CMC) at room temperature. However, under photochemical irradiation, small anisotropic and spherically shaped Ag nanoparticles (3-11 nm) were obtained upon changing the concentration of NaC from below to above the CMC. The matrix-assisted laser desorption ionization time-of-flight and electrospray ionization mass spectra showed that the cluster sample was composed of Ag4 and Ag6. The optical properties of the clusters were studied by UV-visible and luminescence spectroscopy. The lifetime of the synthesized fluorescent Ag nanoclusters (AgNCs) was measured using a time-correlated single-photon counting technique. High-resolution transmission electron microscopy was used to assess the size of clusters and nanoparticles. A protocol for transferring nanoclusters to organic solvents is also described. Toxicity and bioimaging studies of NaC templated AgNCs were conducted using developmental stage zebrafish embryos. From the survival and hatching experiment, no significant toxic effect was observed at AgNC concentrations of up to 200 μL/mL, and the NC-stained embryos exhibited blue fluorescence with high intensity for a long period of time, which shows that AgNCs are more stable in living system.

  6. BLUE STRAGGLERS IN GLOBULAR CLUSTER 47 TUCANAE

    NASA Technical Reports Server (NTRS)

    2002-01-01

    The core of globular cluster 47 Tucanae is home to many blue stragglers, rejuvenated stars that glow with the blue light of young stars. A ground-based telescope image (on the left) shows the entire crowded core of 47 Tucanae, located 15,000 light-years away in the constellation Tucana. Peering into the heart of the globular cluster's bright core, the Hubble Space Telescope's Wide Field and Planetary Camera 2 separated the dense clump of stars into many individual stars (image on right). Some of these stars shine with the light of old stars; others with the blue light of blue stragglers. The yellow circles in the Hubble telescope image highlight several of the cluster's blue stragglers. Analysis for this observation centered on one massive blue straggler. Astronomers theorize that blue stragglers are formed either by the slow merger of stars in a double-star system or by the collision of two unrelated stars. For the blue straggler in 47 Tucanae, astronomers favor the slow merger scenario. This image is a 3-color composite of archival Hubble Wide Field and Planetary Camera 2 images in the ultraviolet (blue), blue (green), and violet (red) filters. Color tables were assigned and scaled so that the red giant stars appear orange, main-sequence stars are white/green, and blue stragglers are appropriately blue. The ultraviolet images were taken on Oct. 25, 1995, and the blue and violet images were taken on Sept. 1, 1995. Credit: Rex Saffer (Villanova University) and Dave Zurek (STScI), and NASA

  7. Streaking images that appear only in the plane of diffraction in undoped GaAs single crystals: Diffraction imaging (topography) by monochromatic synchrotron radiation

    NASA Technical Reports Server (NTRS)

    Kuriyama, Masao; Steiner, Bruce; Dobbyn, Ronald C.; Laor, Uri; Larson, David; Brown, Margaret

    1988-01-01

    Streaking images restricted to the direction of the diffraction (scattering) vector have been observed on transmission through undoped GaAs. These disruption images (caused by the reduction of diffraction in the direction of observation) appear both in the forward and in Bragg diffracted directions in monochromatic synchrontron radiation diffraction imaging. This previously unobserved phenomenon can be explained in terms of planar defects (interfaces) or platelets which affects the absorption coefficient in anomalous transmission. Such regions of the crystal look perfect despite the presence of imperfections when the scattering vector is not perpendicular to the normal of the platelets. The observed crystallographic orientation of these interfaces strongly indicates that they are antiphase boundaries.

  8. On the image of AGS 3He2+ + $\\vec{n}$0 in the blue

    SciTech Connect

    Meot, F.; Huang, H.; Tsoupas, N.

    2015-03-01

    This note addresses the transport of Helion spin $\\vec{n}$0 vector, from its periodic orientation in the AGS to RHIC Blue ring injection kicker, via the AGS extraction system and the AtR line. The goal is to investigate optimal injection energy into RHIC, in the matter of Helion spin matching, in the hypothesis of equal warm and cold snake strengths in the AGS. The study uses recently computed OPERA 3-D field maps of the AGS cold snake, including possibility of independent solenoid and helix settings (as discussed in Tech. Note C-A/AP/485), together with the machinery of the AGS and AtR models developed in the stepwise ray-tracing code Zgoubi. Computing tools and methods employed are discussed as well, in order to facilitate possible further checks or investigations. They are however similar to those used in an earlier study regarding the image in RHIC Blue and Yellow of AGS $\\vec{n}$0 via the AtR in the case of proton beam (Tech. Note C-A/AP/502), which can be referred to for additional details.

  9. THE DEEP BLUE COLOR OF HD 189733b: ALBEDO MEASUREMENTS WITH HUBBLE SPACE TELESCOPE/SPACE TELESCOPE IMAGING SPECTROGRAPH AT VISIBLE WAVELENGTHS

    SciTech Connect

    Evans, Thomas M.; Aigrain, Suzanne; Barstow, Joanna K.; Pont, Frederic; Sing, David K.; Desert, Jean-Michel; Knutson, Heather A.; Gibson, Neale; Heng, Kevin; Lecavelier des Etangs, Alain

    2013-08-01

    We present a secondary eclipse observation for the hot Jupiter HD 189733b across the wavelength range 290-570 nm made using the Space Telescope Imaging Spectrograph on the Hubble Space Telescope. We measure geometric albedos of A{sub g} = 0.40 {+-} 0.12 across 290-450 nm and A{sub g} < 0.12 across 450-570 nm at 1{sigma} confidence. The albedo decrease toward longer wavelengths is also apparent when using six wavelength bins over the same wavelength range. This can be interpreted as evidence for optically thick reflective clouds on the dayside hemisphere with sodium absorption suppressing the scattered light signal beyond {approx}450 nm. Our best-fit albedo values imply that HD 189733b would appear a deep blue color at visible wavelengths.

  10. Assessment of anterior-posterior jaw relationships in Korean adults using the nasion true vertical plane in cone-beam computed tomography images

    PubMed Central

    Park, Youngju; Cho, Youngserk; Mah, James

    2016-01-01

    Objective The aims of this study were to investigate a simple method for assessing anterior-posterior jaw relationships via cone-beam computed tomography (CBCT) images taken in the natural head position (NHP) relative to the nasion true vertical plane (NTVP), and measure normative data in Korean adults with normal profiles. Methods Subjects were selected from patients presenting for third molar extraction and evaluated as having normal profiles by three examiners. The CBCT images of 80 subjects (39 males, 41 females) were taken in the NHP according to Solow and Tallgren's method. Linear measurements of the A-point, B-point, and Pog were calculated relative to the NTVP. Student's t-test was used to assess sexual differences in these measurements. Results The mean linear measurements of the A-point, B-point, and Pog relative to the NTVP were 0.18 mm (standard deviation [SD], 4.77 mm), −4.00 mm (SD, 6.62 mm), and −2.49 mm (SD, 7.14 mm) respectively in Korean males, and 1.48 mm (SD, 4.21 mm), −4.07 mm (SD, 6.70 mm) and −2.91 mm (SD, 7.25 mm) in Korean females respectively. There were no statistically significant differences between Korean males and females (p < 0.05). Conclusions Three-dimensional CBCT analysis using the NTVP is a simple and reliable method for assessing anterior-posterior skeletal relationships. PMID:27226962

  11. The Blue Marble

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This spectacular Moderate Resolution Imaging Spectroradiometer (MODIS) 'blue marble' image is based on the most detailed collection of true-color imagery of the entire Earth to date. Using a collection of satellite-based observations, scientists and visualizers stitched together months of observations of the land surface, oceans, sea ice, and clouds into a seamless, true-color mosaic of every square kilometer (.386 square mile) of our planet. Most of the information contained in this image came from MODIS, illustrating MODIS' outstanding capacity to act as an integrated tool for observing a variety of terrestrial, oceanic, and atmospheric features of the Earth. The land and coastal ocean portions of this image is based on surface observations collected from June through September 2001 and combined, or composited, every eight days to compensate for clouds that might block the satellite's view on any single day. Global ocean color (or chlorophyll) data was used to simulate the ocean surface. MODIS doesn't measure 3-D features of the Earth, so the surface observations were draped over topographic data provided by the U.S. Geological Survey EROS Data Center. MODIS observations of polar sea ice were combined with observations of Antarctica made by the National Oceanic and Atmospheric Administration's AVHRR sensor-the Advanced Very High Resolution Radiometer. The cloud image is a composite of two days of MODIS imagery collected in visible light wavelengths and a third day of thermal infra-red imagery over the poles. A large collection of imagery based on the blue marble in a variety of sizes and formats, including animations and the full (1 km) resolution imagery, is available at the Blue Marble page. Image by Reto Stockli, Render by Robert Simmon. Based on data from the MODIS Science Team

  12. Prussian blue/serum albumin/indocyanine green as a multifunctional nanotheranostic agent for bimodal imaging guided laser mediated combinatorial phototherapy.

    PubMed

    Sahu, Abhishek; Lee, Jong Hyun; Lee, Hye Gyeong; Jeong, Yong Yeon; Tae, Giyoong

    2016-08-28

    Developing novel nanotheranostic agent using only clinically approved materials is highly desirable and challenging. In this study, we combined three clinically approved materials, Prussian blue (PB), serum albumin (BSA), and indocyanine green (ICG), by a simple and biocompatible method to prepare a multifunctional theranostic PB-BSA-ICG nanoparticle. The multifunctional nanoparticle system could provide dual mode magnetic resonance (MR) and near infrared (NIR) fluorescence imaging as well as combined photothermal and photodynamic (PTT-PDT) therapy in response to a single NIR laser. This nanoparticle showed an excellent stability in physiological solutions and could suppress the photo-instability of ICG. In the absence of light, the nanoparticles showed no cytotoxicity, but significant cell death was induced through combined PTT-PDT effect after irradiation with NIR laser light. A high tumor accumulation and minimal nonspecific uptake by other major organs of PB-BSA-ICG nanoparticle were observed in vivo, analyzed by T1-weighted MR and NIR fluorescence bimodal imaging in tumor xenograft mice after intravenous injection. The nanoparticles efficiently suppressed the tumor growth through combinatorial phototherapy with no tumor recurrence upon a single NIR laser irradiation. These results demonstrated that PB-BSA-ICG is potentially an interesting nanotheranostic agent for imaging guided cancer therapy by overcoming the limitations of each technology and enhancing the therapeutic efficiency as well as reducing side effects.

  13. Comparison of computerized image analysis with traditional semiquantitative scoring of Perls' Prussian Blue stained hepatic iron deposition.

    PubMed

    Hall, A Peter; Davies, Wendy; Stamp, Katie; Clamp, Isabel; Bigley, Alison

    2013-01-01

    Image analysis is now routinely employed as a tool in toxicologic pathology to help quantitate end points of efficacy and safety. It is regarded as a proficient and a sensitive technique to generate numerical data that can be easily interrogated for statistical evaluation. Traditional semiquantitative pathology scoring on the other hand is sometimes regarded as less accurate due to the limitations of the scoring systems employed and the day-to-day variations often noted between pathologists. We therefore decided to generate an optimized histochemical staining and image analysis protocol to compare the accuracy of semiquantitative scoring with computerized image analysis. In order to achieve this, we describe a standardized protocol for staining and image analysis that eliminates or minimizes as many sources of error as possible. The results of this experiment demonstrate that despite consistent variations in scoring between two independent pathologists, correlation with image analysis data of 0.91 to 0.95 (Spearman's Rho test) was achieved. These data indicate that either image analysis or traditional semiquantitative scoring can generate accurate data. As a result of this, it appears that it is equally safe to employ either method dependent upon the complexity and the practicality of the task at hand provided that the experimental conditions are rigorously optimized and rigidly adhered to.

  14. Isolating stem cells in the inter-follicular epidermis employing synchrotron radiation-based Fourier-transform infrared microspectroscopy and focal plane array imaging.

    PubMed

    Patel, Imran I; Harrison, Wesley J; Kerns, Jemma G; Filik, Jacob; Wehbe, Katia; Carmichael, Paul L; Scott, Andrew D; Philpott, Mike P; Frogley, Mark D; Cinque, Gianfelice; Martin, Francis L

    2012-10-01

    Normal function and physiology of the epidermis is maintained by the regenerative capacity of this tissue via adult stem cells (SCs). However, definitive identifying markers for SCs remain elusive. Infrared (IR) spectroscopy exploits the ability of cellular biomolecules to absorb in the mid-IR region (λ = 2.5-25 μm), detecting vibrational transitions of chemical bonds. In this study, we exploited the cell's inherent biochemical composition to discriminate SCs of the inter-follicular skin epidermis based on IR-derived markers. Paraffin-embedded samples of human scalp skin (n = 4) were obtained, and 10-μm thick sections were mounted for IR spectroscopy. Samples were interrogated in transmission mode using synchrotron radiation-based Fourier-transform IR (FTIR) microspectroscopy (15 × 15 μm) and also imaged employing globar-source FTIR focal plane array (FPA) imaging (5.4 × 5.4 μm). Dependent on the location of derived spectra, wavenumber-absorbance/intensity relationships were examined using unsupervised principal component analysis. This approach showed clear separation and spectral differences dependent on cell type. Spectral biomarkers concurrently associated with segregation of SCs, transit-amplifying cells and terminally-differentiated cells of epidermis were primarily PO(2)(-) vibrational modes (1,225 and 1,080 cm(-1)), related to DNA conformational alterations. FPA imaging coupled with hierarchical cluster analysis also indicated the presence of specific basal layer cells potentially originating from the follicular bulge, suggested by co-clustering of spectra. This study highlights PO (2) (-) vibrational modes as potential putative SC markers.

  15. The Blue Bottle Revisited.

    ERIC Educational Resources Information Center

    Vandaveer, Walter R., IV; Mosher, Mel

    1997-01-01

    Presents a modification of the classic Blue Bottle demonstration that involves the alkaline glucose reduction of methylene blue. Uses other indicators in the classic Blue Bottle to produce a rainbow of colors. (JRH)

  16. Blue-phase liquid crystal cored optical fiber array with photonic bandgaps and nonlinear transmission properties.

    PubMed

    Khoo, Iam Choon; Hong, Kuan Lung; Zhao, Shuo; Ma, Ding; Lin, Tsung-Hsien

    2013-02-25

    Blue-phase liquid crystal (BPLC) is introduced into the pores of capillary arrays to fabricate fiber arrays. Owing to the photonic-crystals like properties of BPLC, these fiber arrays exhibit temperature dependent photonic bandgaps in the visible spectrum. With the cores maintained in isotropic as well as the Blue phases, the fiber arrays allow high quality image transmission when inserted in the focal plane of a 1x telescope. Nonlinear transmission and optical limiting action on a cw white-light continuum laser is also observed and is attributed to laser induced self-defocusing and propagation modes changing effects caused by some finite absorption of the broadband laser at the short wavelength regime. These nonlinear and other known electro-optical properties of BPLC, in conjunction with their fabrication ease make these fiber arrays highly promising for imaging, electro-optical or all-optical modulation, switching and passive optical limiting applications.

  17. Two‐dimensional‐NGC‐SENSE‐GRAPPA for fast, ghosting‐robust reconstruction of in‐plane and slice‐accelerated blipped‐CAIPI echo planar imaging

    PubMed Central

    2016-01-01

    Purpose Ghosting‐robust reconstruction of blipped‐CAIPI echo planar imaging simultaneous multislice data with low computational load. Methods To date, Slice‐GRAPPA, with “odd–even” kernels that improve ghosting performance, has been the framework of choice for such reconstructions due to its predecessor SENSE‐GRAPPA being deemed unsuitable for blipped‐CAIPI data. Modifications to SENSE‐GRAPPA are used to restore CAIPI compatibility and to make it robust against ghosting. Two implementations are tested, one where slices and in‐plane unaliasing are dealt in the same serial manner as in Slice‐GRAPPA [referred to as one‐dimensional (1D)‐NGC‐SENSE‐GRAPPA, where NGC stands for Nyquist Ghost Corrected] and one where both are unaliased in a single step (2D‐NGC‐SENSE‐GRAPPA), which is analytically and experimentally shown to be computationally cheaper. Results The 1D‐NGC‐SENSE‐GRAPPA and odd‐even Slice‐GRAPPA perform identically, whereas 2D‐NGC‐SENSE‐GRAPPA shows reduced error propagation, less residual ghosting when reliable reference data were available. When the latter was not the case, error propagation was increased. Conclusion Unlike Slice‐GRAPPA, SENSE‐GRAPPA operates fully within the GRAPPA framework, for which improved reconstructions (e.g., iterative, nonlinear) have been developed over the past decade. It could, therefore, bring benefit to the reconstruction of SMS data as an attractive alternative to Slice‐GRAPPA. Magn Reson Med 77:998–1009, 2017. © 2016 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. PMID:26932565

  18. Delayed Methylene Blue Improves Lesion Volume, Multi-Parametric Quantitative Magnetic Resonance Imaging Measurements, and Behavioral Outcome after Traumatic Brain Injury.

    PubMed

    Talley Watts, Lora; Long, Justin Alexander; Boggs, Robert Cole; Manga, Hemanth; Huang, Shiliang; Shen, Qiang; Duong, Timothy Q

    2016-01-15

    Traumatic brain injury (TBI) remains a primary cause of death and disability in both civilian and military populations worldwide. There is a critical need for the development of neuroprotective agents that can circumvent damage and provide functional recovery. We previously showed that methylene blue (MB), a U.S. Food and Drug Administration-grandfathered drug with energy-enhancing and antioxidant properties, given 1 and 3 h post-TBI, had neuroprotective effects in rats. This study aimed to further investigate the neuroprotection of delayed MB treatment (24 h postinjury) post-TBI as measured by lesion volume and functional outcomes. Comparisons were made with vehicle and acute MB treatment. Multi-modal magnetic resonance imaging and behavioral studies were performed at 1 and 3 h and 2, 7, and 14 days after an impact to the primary forelimb somatosensory cortex. We found that delaying MB treatment 24 h postinjury still minimized lesion volume and functional deficits, compared to vehicle-treated animals. The data further support the potential for MB as a neuroprotective treatment, especially when medical teatment is not readily available. MB has an excellent safety profile and is clinically approved for other indications. MB clinical trials on TBI can thus be readily explored.

  19. A Hot Companion to a Blue Straggler in NGC 188 as Revealed by the Ultra-Violet Imaging Telescope (UVIT) on ASTROSAT

    NASA Astrophysics Data System (ADS)

    Subramaniam, Annapurni; Sindhu, N.; Tandon, S. N.; Kameswara Rao, N.; Postma, J.; Côté, Patrick; Hutchings, J. B.; Ghosh, S. K.; George, K.; Girish, V.; Mohan, R.; Murthy, J.; Sankarasubramanian, K.; Stalin, C. S.; Sutaria, F.; Mondal, C.; Sahu, S.

    2016-12-01

    We present early results from the Ultra-Violet Imaging Telescope (UVIT) on board the ASTROSAT observatory. We report the discovery of a hot companion associated with one of the blue straggler stars (BSSs) in the old open cluster, NGC 188. Using fluxes measured in four filters in UVIT’s far-UV (FUV) channel, and two filters in the near-UV (NUV) channel, we have constructed the spectral energy distribution (SED) of the star WOCS-5885, after combining with flux measurements from GALEX, Ultraviolet Imaging Telescope, Ultraviolet Optical Telescope, SPITZER, WISE, and several ground-based facilities. The resulting SED spans a wavelength range of 0.15 μm to 7.8 μm. This object is found to be one of the brightest FUV sources in the cluster. An analysis of the SED reveals the presence of two components. The cooler component is found to have a temperature of 6000 ± 150 K, confirming that it is a BSS. Assuming it to be a main-sequence star, we estimate its mass to be ˜1.1-1.2 M ⊙. The hotter component, with an estimated temperature of 17,000 ± 500 K, has a radius of ˜ 0.6 R ⊙ and L ˜30 L ⊙. Bigger and more luminous than a white dwarf, yet cooler than a sub-dwarf, we speculate that it is a post-AGB/HB star that has recently transferred its mass to the BSS, which is known to be a rapid rotator. This binary system, which is the first BSS with a post-AGB/HB companion identified in an open cluster, is an ideal laboratory to study the process of BSS formation via mass transfer.

  20. A high-NA solid immersion objective for imaging a Blue-ray disc and investigating subsurface damage

    NASA Astrophysics Data System (ADS)

    Densmore, Victor; Kim, Young Sik; Milster, Tom; Watson, Matt; Kwok, Dolaphine

    2014-09-01

    Nano-scale resolution in miniature optical systems has been realized in the optical data storage industry. Numerical apertures greater than unity have been achieved in by utilizing the high index material of a hemispherical Solid Immersion Lens (SIL), which increases the resolution of the backing objective by a factor that is related to the refractive index of the SIL. In this research, a custom Hyper-Blu-Disc (HBD) NA=1.4 SIL objective is utilized for high-fidelity readout of data pits beneath a 100μm thick cover layer on an optical Blu-Ray Disc. If realized commercially, the increase in data density could be 3X today's Blu-Ray technology. A distinct difference between this work and other work with SILs in optical data storage is the relatively thick cover layer of 100μm. Recently, there has been interest in discovering new ways to apply the technology and methods used in optical data storage for other means. The inherent design of the HBD objective to image through a shallow layer of dielectric material may lend itself to be used as an effective means for characterizing subsurface damage in optical materials. This research will furthermore investigate the HBD objective as a means of detecting subsurface damage.

  1. Preparation of multi-coloured different sized fluorescent gold clusters from blue to NIR, structural analysis of the blue emitting Au7 cluster, and cell-imaging by the NIR gold cluster.

    PubMed

    Roy, Subhasish; Baral, Abhishek; Bhattacharjee, Rameswar; Jana, Batakrishna; Datta, Ayan; Ghosh, Surajit; Banerjee, Arindam

    2015-02-07

    Blue, green, orange-red, red and NIR emitting gold quantum clusters have been prepared in aqueous media by using a bioactive peptide glutathione (reduced) at physiological pH. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analyses show that the core structure sizes of the five different gold clusters are Au7 (blue), Au16 (green), Au19 (orange-red), Au21 (red) and Au22 (NIR). The photo-stability and pH-stability of these quantum clusters have been measured, and these are photo-stable against continuous UV irradiation for a few hours. They also exhibit moderate to good pH-stability within the pH range of 5-12.5. A computational study reveals the organisation of gold atoms in the thiolate-protected blue quantum cluster and its several structural parameters, including the mode of interaction of ligand molecules with Au atoms in the Au7 cluster. Interestingly, it has been found that NIR emitting gold quantum cluster can easily be internalized into the adenocarcinomic human alveolar basal epithelial cell line (A549 cell line). Moreover, a MTT assay indicates that our NIR emitting gold quantum cluster show very low cytotoxicy to A549 cancer cells.

  2. Double plane wave reverse time migration with plane wave Green's function

    NASA Astrophysics Data System (ADS)

    Zhao, Z.; Sen, M. K.; Stoffa, P. L.

    2015-12-01

    Reverse time migration (RTM) is effective in obtaining complex subsurface structures from seismic data. By solving the two-way wave equation, RTM can use entire wavefield for imaging. Although powerful computer are becoming available, the conventional pre-stack shot gather RTM is still computationally expensive. Solving forward and backward wavefield propagation for each source location and shot gather is extremely time consuming, especially for large seismic datasets. We present an efficient, accurate and flexible plane wave RTM in the frequency domain where we utilize a compressed plane wave dataset, known as the double plane wave (DPW) dataset. Provided with densely sampled seismic dataset, shot gathers can be decomposed into source and receiver plane wave components with minimal artifacts. The DPW RTM is derived under the Born approximation and utilizes frequency domain plane wave Green's function for imaging. Time dips in the shot profiles can help to estimate the range of plane wave components present in shot gathers. Therefore, a limited number of plane wave Green's functions are needed for imaging. Plane wave Green's functions can be used for imaging both source and receiver plane waves. Source and receiver reciprocity can be used for imaging plane wave components at no cost and save half of the computation time. As a result, the computational burden for migration is substantially reduced. Plane wave components can be migrated independently to recover specific targets with given dips, and ray parameter common image gathers (CIGs) can be generated after migration directly. The ray parameter CIGs can be used to justify the correctness of velocity models. Subsurface anisotropy effects can also be included in our imaging condition, provided with plane wave Green's functions in the anisotropic media.

  3. Identification of microplastic in effluents of waste water treatment plants using focal plane array-based micro-Fourier-transform infrared imaging.

    PubMed

    Mintenig, S M; Int-Veen, I; Löder, M G J; Primpke, S; Gerdts, G

    2017-01-01

    The global presence of microplastic (MP) in aquatic ecosystems has been shown by various studies. However, neither MP concentrations nor their sources or sinks are completely known. Waste water treatment plants (WWTPs) are considered as significant point sources discharging MP to the environment. This study investigated MP in the effluents of 12 WWTPs in Lower Saxony, Germany. Samples were purified by a plastic-preserving enzymatic-oxidative procedure and subsequent density separation using a zinc chloride solution. For analysis, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FT-IR) and focal plane array (FPA)-based transmission micro-FT-IR imaging were applied. This allowed the identification of polymers of all MP down to a size of 20 μm. In all effluents MP was found with quantities ranging from 0 to 5 × 10(1) m(-3) MP > 500 μm and 1 × 10(1) to 9 × 10(3) m(-3) MP < 500 μm. By far, polyethylene was the most frequent polymer type in both size classes. Quantities of synthetic fibres ranged from 9 × 10(1) to 1 × 10(3) m(-3) and were predominantly made of polyester. Considering the annual effluxes of tested WWTPs, total discharges of 9 × 10(7) to 4 × 10(9) MP particles and fibres per WWTP could be expected. Interestingly, one tertiary WWTP had an additionally installed post-filtration that reduced the total MP discharge by 97%. Furthermore, the sewage sludge of six WWTPs was examined and the existence of MP, predominantly polyethylene, revealed. Our findings suggest that WWTPs could be a sink but also a source of MP and thus can be considered to play an important role for environmental MP pollution.

  4. A 128×96 Pixel Stack-Type Color Image Sensor: Stack of Individual Blue-, Green-, and Red-Sensitive Organic Photoconductive Films Integrated with a ZnO Thin Film Transistor Readout Circuit

    NASA Astrophysics Data System (ADS)

    Seo, Hokuto; Aihara, Satoshi; Watabe, Toshihisa; Ohtake, Hiroshi; Sakai, Toshikatsu; Kubota, Misao; Egami, Norifumi; Hiramatsu, Takahiro; Matsuda, Tokiyoshi; Furuta, Mamoru; Hirao, Takashi

    2011-02-01

    A color image was produced by a vertically stacked image sensor with blue (B)-, green (G)-, and red (R)-sensitive organic photoconductive films, each having a thin-film transistor (TFT) array that uses a zinc oxide (ZnO) channel to read out the signal generated in each organic film. The number of the pixels of the fabricated image sensor is 128×96 for each color, and the pixel size is 100×100 µm2. The current on/off ratio of the ZnO TFT is over 106, and the B-, G-, and R-sensitive organic photoconductive films show excellent wavelength selectivity. The stacked image sensor can produce a color image at 10 frames per second with a resolution corresponding to the pixel number. This result clearly shows that color separation is achieved without using any conventional color separation optical system such as a color filter array or a prism.

  5. Perilesional Inflammation in Neurocysticercosis - Relationship Between Contrast-Enhanced Magnetic Resonance Imaging, Evans Blue Staining and Histopathology in the Pig Model

    PubMed Central

    Bustos, Javier A.; Calcina, Juan; Vargas-Calla, Ana; Suarez, Diego; Gonzalez, Armando E.; Chacaltana, Juan; Guerra-Giraldez, Cristina; Mahanty, Siddhartha; Nash, Theodore E.; García, Hector H.

    2016-01-01

    Background Disease manifestations in neurocysticercosis (NCC) are frequently due to inflammation of degenerating Taenia solium brain cysts. Exacerbated inflammation post anthelmintic treatment is associated with leakage of the blood brain barrier (BBB) using Evans blue (EB) staining. How well EB extravasation into the brain correlates with magnetic resonance imaging (MRI) using gadolinium (Gd) enhancement as a contrast agent and pericystic inflammation was analyzed in pigs harboring brain cysts of Taenia solium. Methodology/Principal Findings Three groups of 4 naturally infected pigs were assessed. The first and second groups were treated with both praziquantel plus albendazole and sacrificed two and five days post treatment, respectively. A third untreated group remained untreated. Pigs were injected with EB two hours prior to evaluation by Gd-enhanced T1-MRI, and euthanized. The EB staining for each cyst capsule was scored (EB grades were 0: 0%; 1: up to 50%; 2: over 50% but less than 100%; 3: 100%). Similarly, the Gd enhancement around each cyst was qualitatively and quantitatively scored from the MRI. The extent of pericystic inflammation on histology was scored in increasing severity as IS1, IS2, IS3 and IS4. Grade 3 EB staining and enhancement was only seen in treated capsules. Also, treated groups had higher Gd intensity than the untreated group. Grades of enhancement correlated significantly with Gd enhancement intensity. EB staining was correlated with Gd enhancement intensity and with IS4 in the treated groups. These correlations were stronger in internally located cysts compared to superficial cysts in treated groups. Significance EB staining and Gd enhancement strongly correlate. The intensity of enhancement determined by MRI is a good indication of the degree of inflammation. Similarly, EB staining highly correlates with the degree of inflammation and may be applied to study inflammation in the pig model of NCC. PMID:27459388

  6. An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy

    NASA Astrophysics Data System (ADS)

    Wollman, Adam J. M.; Miller, Helen; Foster, Simon; Leake, Mark C.

    2016-10-01

    Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image analysis framework for automated detection and image segmentation of cells in S. aureus cell clusters, and explicit identification of their cell division planes. We use a new combination of several existing analytical tools of image analysis to detect cellular and subcellular morphological features relevant to cell division from millisecond time scale sampled images of live pathogens at a detection precision of single molecules. We demonstrate this approach using a fluorescent reporter GFP fused to the protein EzrA that localises to a mid-cell plane during division and is involved in regulation of cell size and division. This image analysis framework presents a valuable platform from which to study candidate new antimicrobials which target the cell division machinery, but may also have more general application in detecting morphologically complex structures of fluorescently labelled proteins present in clusters of other types of cells.

  7. SU-C-18C-02: Specifcation of X-Ray Projection Angles Which Are Aligned with the Aortic Valve Plane From a Planar Image of a Valvuloplasty Balloon Inflated Across the Aortic Valve

    SciTech Connect

    Fetterly, K; Mathew, V

    2014-06-01

    Purpose: Transcatheter aortic valve replacement (TAVR) procedures provide a method to implant a prosthetic aortic valve via a minimallyinvasive, catheter-based procedure. TAVR procedures require use of interventional fluoroscopy c-arm projection angles which are aligned with the aortic valve plane to minimize prosthetic valve positioning error due to x-ray imaging parallax. The purpose of this work is to calculate the continuous range of interventional fluoroscopy c-arm projection angles which are aligned with the aortic valve plane from a single planar image of a valvuloplasty balloon inflated across the aortic valve. Methods: Computational methods to measure the 3D angular orientation of the aortic valve were developed. Required inputs include a planar x-ray image of a known valvuloplasty balloon inflated across the aortic valve and specifications of x-ray imaging geometry from the DICOM header of the image. A-priori knowledge of the species-specific typical range of aortic orientation is required to specify the sign of the angle of the long axis of the balloon with respect to the x-ray beam. The methods were validated ex-vivo and in a live pig. Results: Ex-vivo experiments demonstrated that the angular orientation of a stationary inflated valvuloplasty balloon can be measured with precision less than 1 degree. In-vivo pig experiments demonstrated that cardiac motion contributed to measurement variability, with precision less than 3 degrees. Error in specification of x-ray geometry directly influences measurement accuracy. Conclusion: This work demonstrates that the 3D angular orientation of the aortic valve can be calculated precisely from a planar image of a valvuloplasty balloon inflated across the aortic valve and known x-ray geometry. This method could be used to determine appropriate c-arm angular projections during TAVR procedures to minimize x-ray imaging parallax and thereby minimize prosthetic valve positioning errors.

  8. The Blue Water

    ERIC Educational Resources Information Center

    Berger, J. Joel

    1973-01-01

    Describes some of the advantages of an elementary science activity in which students discover that blowing through a straw into a bromthymol blue solution changes the color to yellow. Directions are provided for preparing the bromthymol blue solution. (JR)

  9. Blue nightshade poisoning

    MedlinePlus

    ... when someone eats parts of the blue nightshade plant. This article is for information only. DO NOT ... is found in the blue nightshade ( Solanum dulcamara ) plant, especially in the fruit and leaves.

  10. Mosaic focal plane for star sensors

    NASA Astrophysics Data System (ADS)

    Chang, N. C.

    1981-02-01

    The basic principles of star sensors are reviewed with reference to the advantages of replacing photodiodes, image dissectors, and vidicons with mosaic charge transfer device (CTD) focal planes. The desirable characteristics of CTD focal planes include: high uniformity, high transfer effect, low dark current, low hot and cold spots, low dead space, low angular misalignment, high coplanarity, and high thermal stability. An implementation of a mosaic CTD array star sensor which achieves high angular position accuracy and frequency attitude update is presented. Two focal plane packaging concepts, the planar and vertical board packagings, are examined.

  11. Analysis of the Maillard reaction in human hair using Fourier transform infrared spectroscopic imaging and a focal-plane array detector.

    PubMed

    Jung, In-Keun; Park, Sang-Chul; Bin, Sung-Ah; Roh, Young Sup; Lee, John Hwan; Kim, Boo-Min

    2016-03-01

    The Maillard reaction has been well researched and used in the food industry and the fields of environmental science and organic chemistry. Here, we induced the Maillard reaction inside human hair and analyzed its effects by using Fourier transform infrared spectroscopy with a focal-plane array (FTIR-FPA) detector. We used arginine (A), glycine (G), and D-xylose (X) to generate the Maillard reaction by dissolving them in purified water and heating it to 150 °C. This label-free process generated a complex compound (named AGX after its ingredients) with a monomer structure, which was determined by using nuclear magnetic resonance (NMR) and FTIR-FPA. This compound was stable in hair and substantially increased its tensile strength. To our knowledge, we are the first to report the formation of this monomer in human hair, and our study provides insights into a new method that could be used to improve the condition of damaged or aging hair.

  12. Image Calibration

    NASA Technical Reports Server (NTRS)

    Peay, Christopher S.; Palacios, David M.

    2011-01-01

    Calibrate_Image calibrates images obtained from focal plane arrays so that the output image more accurately represents the observed scene. The function takes as input a degraded image along with a flat field image and a dark frame image produced by the focal plane array and outputs a corrected image. The three most prominent sources of image degradation are corrected for: dark current accumulation, gain non-uniformity across the focal plane array, and hot and/or dead pixels in the array. In the corrected output image the dark current is subtracted, the gain variation is equalized, and values for hot and dead pixels are estimated, using bicubic interpolation techniques.

  13. Visualization of microvascular proliferation as a tumor infiltration structure in rat glioma specimens using the diffraction-enhanced imaging in-plane CT technique

    NASA Astrophysics Data System (ADS)

    Seo, Seung-Jun; Sunaguchi, Naoki; Yuasa, Tetsuya; Huo, Qingkai; Ando, Masami; Choi, Gi-Hwan; Kim, Hong-Tae; Kim, Ki-Hong; Jeong, Eun-Ju; Chang, Won-Seok; Kim, Jong-Ki

    2012-03-01

    In order to study potent microenvironments of malignant gliomas with a high- resolution x-ray imaging technique, an injection orthotopic glioma model was made using the Sprague-Dawley rat. Total brain tissue, taken out as an ex vivo model, was examined with diffraction-enhanced imaging (DEI) computed tomography (CT) acquired with a 35 keV monochromatic x-ray. In the convolution-reconstructed 2D/3D images with a spatial resolution of 12.5 × 12.5 × 25 µm, distinction among necrosis, typical ring-shaped viable tumors, edemas and healthy tissues was clearly observed near the frontal lobe in front of the rat's caudate nucleus. Multiple microvascular proliferations (MVPs) were observed surrounding peritumoral edemas as a tumor infiltration structure. Typical dimensions of tubular MVPs were 130 (diameter) ×250 (length) µm with a partial sprout structure revealed in the 3D reconstructed image. Hyperplasia of cells around vessel walls was revealed with tumor cell infiltration along the perivascular space in microscopic observations of mild MVP during histological analysis. In conclusion, DEI-CT is capable of imaging potent tumor-infiltrating MVP structures surrounding high-grade gliomas.

  14. SNAP Satellite Focal Plane Development

    SciTech Connect

    Bebek, C.; Akerlof, C.; Aldering, G.; Amanullah, R.; Astier, P.; Baltay, C.; Barrelet, E.; Basa, S.; Bercovitz, J.; Bergstrom, L.; Berstein, G.P.; Bester, M.; Bohlin, R.; Bonissent, A.; Bower, C.; Campbell, M.; Carithers, W.; Commins, E.; Day, C.; Deustua, S.; DiGennaro, R.; Ealet, A.; Ellis, R.; Emmett, W.; Eriksson, M.; Fouchez,D.; Fruchter, A.; Genat, J-F.; Goldhaber, G.; Goobar, A.; Groom, D.; Heetderks, H.; Holland, S.; Huterer, D.; Johnson, W.; Kadel, R.; Karcher,A.; Kim, A.; Kolbe, W.; Lafever, R.; Lamoureaux, J.; Lampton, M.; Lefevre, O.; Levi, M.; Levin, D.; Linder, E.; Loken, S.; Malina, R.; Mazure, A.; McKay, T.; McKee, S.; Miquel, R.; Morgan, N.; Mortsell, E.; Mostek, N.; Mufson, S.; Musser, J.; Roe, N.; Nugent, P.; Oluseyi, H.; Pain, R.; Palaio, N.; Pankow, D.; Perlmutter, S.; Prieto, E.; Rabinowitz,D.; Refregier, A.; Rhodes, J.; Schubnell, M.; Sholl, M.; Smadja, G.; Smith, R.; Smoot, G.; Snyder, J.; Spadafora, A.; Szymkowiak, A.; Tarle,G.; Taylor, K.; Tilquin, A.; Tomasch, A.; Vincent, D.; von der Lippe, H.; Walder, J-P.; Wang, G.

    2003-07-07

    The proposed SuperNova/Acceleration Probe (SNAP) mission will have a two-meter class telescope delivering diffraction-limited images to an instrumented 0.7 square degree field in the visible and near-infrared wavelength regime. The requirements for the instrument suite and the present configuration of the focal plane concept are presented. A two year R&D phase, largely supported by the Department of Energy, is just beginning. We describe the development activities that are taking place to advance our preparedness for mission proposal in the areas of detectors and electronics.

  15. The MSX Galactic Plane Survey Submillimeter Results

    NASA Astrophysics Data System (ADS)

    Price, S.; Carey, S.; Egan, M. P.

    The MidCourse Space eXperiment (MSX) surveyed the Galactic plane within 5° latitude in four mid-infrared spectral bands. A set of full resolution (20'') 1.5^circ×1.5^circ images on 6'' pixel centers has been created in each spectral band by co-adding all the survey data. A lower (1.2') resolution atlas of 10^circ×10^circ images provide large-scale panoramas of the plane. A new class of objects has been identified in the images, infrared dark clouds, which are silhouetted against the mid-infrared background emission from the interstellar medium in the Galactic plane. The IRAS ISSA plates indicate that these clouds are dark out to 100 μm. Submillimeter emission traces the form of the dark cloud and reveals cores indicative of class 0 protostars.

  16. GLAMER - II. Multiple-plane gravitational lensing

    NASA Astrophysics Data System (ADS)

    Petkova, Margarita; Metcalf, R. Benton; Giocoli, Carlo

    2014-12-01

    We present an extension to multiple planes of the gravitational lensing code GLAMER. The method entails projecting the mass in the observed light-cone on to a discrete number of lens planes and inverse ray-shooting from the image to the source plane. The mass on each plane can be represented as haloes, simulation particles, a projected mass map extracted form a numerical simulation or any combination of these. The image finding is done in a source-oriented fashion, where only regions of interest are iteratively refined on an initially coarse image plane grid. The calculations are performed in parallel on shared memory machines. The code is able to handle different types of analytic haloes (NFW, NSIE, power law, etc.), haloes extracted from numerical simulations and clusters constructed from semi-analytic models (MOKA). Likewise, there are several different options for modelling the source(s) which can be distributed throughout the light-cone. The distribution of matter in the light-cone can be either taken from a pre-existing N-body numerical simulations, from halo catalogues, or are generated from an analytic mass function. We present several tests of the code and demonstrate some of its applications such as generating mock images of galaxy and galaxy cluster lenses.

  17. List 47: blue honeysuckle

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This summary presents the descriptions of a newly released blue honeysuckle (Lonicera cerulea L.) cultivar for the List of New Fruit and Nut Cultivars. This blue honeysuckle cultivar was released in Canada in 2012 and has pending Plant Breeder’s Rights Certification with Agriculture Canada. The cult...

  18. Blue Willow Story Plates

    ERIC Educational Resources Information Center

    Fontes, Kris

    2009-01-01

    In the December 1997 issue of "SchoolArts" is a lesson titled "Blue Willow Story Plates" by Susan Striker. In this article, the author shares how she used this lesson with her middle-school students many times over the years. Here, she describes a Blue Willow plate painting project that her students made.

  19. Blue Ocean Thinking

    ERIC Educational Resources Information Center

    Orem, Donna

    2016-01-01

    This article describes a concept called the "blue ocean thinking strategy," developed by W. Chan Kim and Renée Mauborgne, professors at INSEAD, an international graduate school of business in France. The "blue ocean" thinking strategy considers opportunities to create new markets for services, rather than focusing solely on…

  20. Introducing the Blues.

    ERIC Educational Resources Information Center

    Sinclair, Bryan

    2000-01-01

    Discusses the history of the blues and presents a list of resources that are designed to introduce the blues, both as a feeling and as an influential part of American music and culture. Includes picture books and nonfiction for young readers, nonfiction for older readers, Web sites, and compact disks. (LRW)

  1. After Stroke, 'Blue' Light May Help Beat the Blues

    MedlinePlus

    ... Light May Help Beat the Blues Akin to sunlight, it could ward off depression during rehab, study ... facility used "blue" light in its lighting system. Sunlight is humans' largest source of blue-spectrum light, ...

  2. FROM BLUE JEANS TO BLUE GENES

    PubMed Central

    Boon, Laurence M.; Vikkula, Miikka

    2010-01-01

    Cutaneous venous anomalies are common. They are blue in color and vary in size, number and location, and account for the majority of consultations at specialized interdisciplinary clinics for vascular anomalies. Venous lesions are clinically important as they cause pain, dysfunction, destruction of adjacent tissues and esthetic concern. Only resection and sclerotherapy are helpful, although not always curative. Understanding etiopathogenesis could help design animal models and develop novel therapeutic approaches. Dr Mulliken envisioned a project to uncover the genetic basis of an inherited form of venous malformation in a large New England family. Recruitment of two young fellows resulted in a collaborative project that unraveled the searched-for-gene and its mutation. This was an opening for a new era in the field of vascular anomalies. Two blue genes’ mutations were discovered, which account for the majority, if not all, of the inherited forms of venous anomalies, but other genes as well, for rheologically diverse lesions. Differential diagnosis and management has improved, and animal models are being made. This was achieved thanks to Dr Mulliken, who inspired two young investigators in blue jeans to find two blue genes. PMID:19190503

  3. From blue jeans to blue genes.

    PubMed

    Boon, Laurence M; Vikkula, Miikka

    2009-03-01

    Cutaneous venous anomalies are common. They are blue and vary in size, number, and location and account for most consultations at specialized interdisciplinary clinics for vascular anomalies. Venous lesions are clinically important because they cause pain, dysfunction, destruction of adjacent tissues, and esthetic concern. Only resection and sclerotherapy are helpful, although not always curative. Understanding etiopathogenesis could help design animal models and develop novel therapeutic approaches. John B. Mulliken, MD, envisioned a project to uncover the genetic basis of an inherited form of venous malformation in a large New England family. Recruitment of 2 young fellows resulted in a collaborative project that unraveled the searched-for gene and its mutation. This was an opening for a new era in vascular anomalies. Two blue genes' mutations were discovered, which account for most, if not all, of the inherited forms of venous anomalies, but other genes as well, for rheologically diverse lesions. Differential diagnosis and management has improved, and animal models are being made. This was achieved through the help of Dr Mulliken, who inspired 2 young investigators in blue jeans to find 2 blue genes.

  4. 50-kHz-rate 2D imaging of temperature and H2O concentration at the exhaust plane of a J85 engine using hyperspectral tomography.

    PubMed

    Ma, Lin; Li, Xuesong; Sanders, Scott T; Caswell, Andrew W; Roy, Sukesh; Plemmons, David H; Gord, James R

    2013-01-14

    This paper describes a novel laser diagnostic and its demonstration in a practical aero-propulsion engine (General Electric J85). The diagnostic technique, named hyperspectral tomography (HT), enables simultaneous 2-dimensional (2D) imaging of temperature and water-vapor concentration at 225 spatial grid points with a temporal response up to 50 kHz. To our knowledge, this is the first time that such sensing capabilities have been reported. This paper introduces the principles of the HT techniques, reports its operation and application in a J85 engine, and discusses its perspective for the study of high-speed reactive flows.

  5. Blue ocean strategy.

    PubMed

    Kim, W Chan; Mauborgne, Renée

    2004-10-01

    Despite a long-term decline in the circus industry, Cirque du Soleil profitably increased revenue 22-fold over the last ten years by reinventing the circus. Rather than competing within the confines of the existing industry or trying to steal customers from rivals, Cirque developed uncontested market space that made the competition irrelevant. Cirque created what the authors call a blue ocean, a previously unknown market space. In blue oceans, demand is created rather than fought over. There is ample opportunity for growth that is both profitable and rapid. In red oceans--that is, in all the industries already existing--companies compete by grabbing for a greater share of limited demand. As the market space gets more crowded, prospects for profits and growth decline. Products turn into commodities, and increasing competition turns the water bloody. There are two ways to create blue oceans. One is to launch completely new industries, as eBay did with online auctions. But it's much more common for a blue ocean to be created from within a red ocean when a company expands the boundaries of an existing industry. In studying more than 150 blue ocean creations in over 30 industries, the authors observed that the traditional units of strategic analysis--company and industry--are of limited use in explaining how and why blue oceans are created. The most appropriate unit of analysis is the strategic move, the set of managerial actions and decisions involved in making a major market-creating business offering. Creating blue oceans builds brands. So powerful is blue ocean strategy, in fact, that a blue ocean strategic move can create brand equity that lasts for decades.

  6. Structure analysis for plane geometry figures

    NASA Astrophysics Data System (ADS)

    Feng, Tianxiao; Lu, Xiaoqing; Liu, Lu; Li, Keqiang; Tang, Zhi

    2013-12-01

    As there are increasing numbers of digital documents for education purpose, we realize that there is not a retrieval application for mathematic plane geometry images. In this paper, we propose a method for retrieving plane geometry figures (PGFs), which often appear in geometry books and digital documents. First, detecting algorithms are applied to detect common basic geometry shapes from a PGF image. Based on all basic shapes, we analyze the structural relationships between two basic shapes and combine some of them to a compound shape to build the PGF descriptor. Afterwards, we apply matching function to retrieve candidate PGF images with ranking. The great contribution of the paper is that we propose a structure analysis method to better describe the spatial relationships in such image composed of many overlapped shapes. Experimental results demonstrate that our analysis method and shape descriptor can obtain good retrieval results with relatively high effectiveness and efficiency.

  7. Precise measurement of planeness.

    PubMed

    Schulz, G; Schwider, J

    1967-06-01

    Interference methods are reviewed-particularly those developed at the German Academy of Sciences in Berlin-with which the deviations of an optically flat surface from the ideal plane can be measured with a high degree of exactness. One aid to achieve this is the relative methods which measure the differences in planeness between two surfaces. These are then used in the absolute methods which determine the absolute planeness of a surface. This absolute determination can be effected in connection with a liquid surface, or (as done by the authors) only by suitable evaluation of relative measurements between unknown plates in various positional combinations. Experimentally, one uses two- or multiple-beam interference fringes of equal thickness(1) or of equal inclination. The fringes are observed visually, scanned, or photographed, and in part several wavelengths or curves of equal density (Aquidensiten) are employed. The survey also brings the following new methods: a relative method, where, with the aid of fringes of superposition, the fringe separation is subdivided equidistantly thus achieving an increase of measuring precision, and an absolute method which determines the deviations of a surface from ideal planeness along arbitrary central sections, without a liquid surface, from four relative interference photographs.

  8. Dual band QWIP focal plane array

    NASA Technical Reports Server (NTRS)

    Gunapala, Sarath D. (Inventor); Choi, Kwong Kit (Inventor); Bandara, Sumith V. (Inventor)

    2005-01-01

    A quantum well infrared photodetector (QWIP) that provides two-color image sensing. Two different quantum wells are configured to absorb two different wavelengths. The QWIPs are arrayed in a focal plane array (FPA). The two-color QWIPs are selected for readout by selective electrical contact with the two different QWIPs or by the use of two different wavelength sensitive gratings.

  9. High speed multi focal plane optical system

    NASA Technical Reports Server (NTRS)

    Minott, P. O. (Inventor)

    1983-01-01

    An apparatus for eliminating beamsplitter generated optical aberrations in a pupil concentric optical system providing a plurality of spatially separated images on different focal planes or surfaces is presented. The system employs a buried surface beamsplitter having spherically curved entrance and exit faces which are concentric to a system aperture stop with the entrance face being located in the path of a converging light beam directed there from an image forming objective element which is also concentric to the aperture stop.

  10. Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

    PubMed Central

    Basu, Koli; Garnham, Christopher P.; Nishimiya, Yoshiyuki; Tsuda, Sakae; Braslavsky, Ido; Davies, Peter

    2014-01-01

    Antifreeze proteins (AFPs) are expressed in a variety of cold-hardy organisms to prevent or slow internal ice growth. AFPs bind to specific planes of ice through their ice-binding surfaces. Fluorescence-based ice plane affinity (FIPA) analysis is a modified technique used to determine the ice planes to which the AFPs bind. FIPA is based on the original ice-etching method for determining AFP-bound ice-planes. It produces clearer images in a shortened experimental time. In FIPA analysis, AFPs are fluorescently labeled with a chimeric tag or a covalent dye then slowly incorporated into a macroscopic single ice crystal, which has been preformed into a hemisphere and oriented to determine the a- and c-axes. The AFP-bound ice hemisphere is imaged under UV light to visualize AFP-bound planes using filters to block out nonspecific light. Fluorescent labeling of the AFPs allows real-time monitoring of AFP adsorption into ice. The labels have been found not to influence the planes to which AFPs bind. FIPA analysis also introduces the option to bind more than one differently tagged AFP on the same single ice crystal to help differentiate their binding planes. These applications of FIPA are helping to advance our understanding of how AFPs bind to ice to halt its growth and why many AFP-producing organisms express multiple AFP isoforms. PMID:24457629

  11. Mongolian blue spots

    MedlinePlus

    ... bruises. This can raise a question about possible child abuse. It is important to recognize that Mongolian blue ... Elsevier Saunders; 2011:chap 11. Read More Benign Child abuse - physical Rashes Review Date 4/14/2015 Updated ...

  12. Methylene blue test

    MedlinePlus

    Methemoglobinemia - methylene blue test ... No special preparation is required for this test. ... which are genetic (problem with your genes). This test is used to tell the difference between methemoglobinemia ...

  13. Blue Ribbon Panel Report

    Cancer.gov

    An NCI Cancer Currents blog by the NCI acting director thanking the cancer community for contributing to the Cancer Moonshot Blue Ribbon Panel report, which was presented to the National Cancer Advisory Board on September 7.

  14. Focal Plane Metrology for the LSST Camera

    SciTech Connect

    A Rasmussen, Andrew P.; Hale, Layton; Kim, Peter; Lee, Eric; Perl, Martin; Schindler, Rafe; Takacs, Peter; Thurston, Timothy; /SLAC

    2007-01-10

    Meeting the science goals for the Large Synoptic Survey Telescope (LSST) translates into a demanding set of imaging performance requirements for the optical system over a wide (3.5{sup o}) field of view. In turn, meeting those imaging requirements necessitates maintaining precise control of the focal plane surface (10 {micro}m P-V) over the entire field of view (640 mm diameter) at the operating temperature (T {approx} -100 C) and over the operational elevation angle range. We briefly describe the hierarchical design approach for the LSST Camera focal plane and the baseline design for assembling the flat focal plane at room temperature. Preliminary results of gravity load and thermal distortion calculations are provided, and early metrological verification of candidate materials under cold thermal conditions are presented. A detailed, generalized method for stitching together sparse metrology data originating from differential, non-contact metrological data acquisition spanning multiple (non-continuous) sensor surfaces making up the focal plane, is described and demonstrated. Finally, we describe some in situ alignment verification alternatives, some of which may be integrated into the camera's focal plane.

  15. An anatomical evaluation of the serratus anterior plane block.

    PubMed

    Mayes, J; Davison, E; Panahi, P; Patten, D; Eljelani, F; Womack, J; Varma, M

    2016-09-01

    The serratus anterior plane block has been described for analgesia of the hemithorax. This study was conducted to determine the spread of injectate and investigate the anatomical basis of the block. Ultrasound-guided serratus anterior plane block was performed on six soft-fix embalmed cadavers. All cadavers received bilateral injections, on one side performed with 20 ml latex and on the other with 20 ml methylene blue. Subsequent dissection explored the extent of spread and nerve involvement. Photographs were taken throughout dissection. The intercostal nerves were involved on three occasions with dye, but not with latex. The lateral cutaneous branches of the intercostal nerve contained dye and latex on all occasions. The serratus plane block appears to be mediated through blockade of the lateral cutaneous branches of the intercostal nerves. Anatomically, serratus plane block does not appear to be equivalent to paravertebral block for rib fracture analgesia.

  16. Parameter Plane Design Method

    DTIC Science & Technology

    1989-03-01

    Th usr a toente aninteer a thca sms b esta 1 Fp-ocsing 2. Enter P1 values, lwgt, ldig - > 9 Table I give us proper values. Table 1. PARAMETER TABLE...necessary and identify by block number) In this thesis a control systems analysis package is developed using parameter plane methods. It is an interactive...designer is able to choose values of the parameters which provide a good compromise between cost and dynamic behavior. 20 Distribution Availability of

  17. Multispectral imaging of absorption and scattering properties of in vivo exposed rat brain using a digital red-green-blue camera

    NASA Astrophysics Data System (ADS)

    Yoshida, Keiichiro; Nishidate, Izumi; Ishizuka, Tomohiro; Kawauchi, Satoko; Sato, Shunichi; Sato, Manabu

    2015-05-01

    In order to estimate multispectral images of the absorption and scattering properties in the cerebral cortex of in vivo rat brain, we investigated spectral reflectance images estimated by the Wiener estimation method using a digital RGB camera. A Monte Carlo simulation-based multiple regression analysis for the corresponding spectral absorbance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) was then used to specify the absorption and scattering parameters of brain tissue. In this analysis, the concentrations of oxygenated hemoglobin and that of deoxygenated hemoglobin were estimated as the absorption parameters, whereas the coefficient a and the exponent b of the reduced scattering coefficient spectrum approximated by a power law function were estimated as the scattering parameters. The spectra of absorption and reduced scattering coefficients were reconstructed from the absorption and scattering parameters, and the spectral images of absorption and reduced scattering coefficients were then estimated. In order to confirm the feasibility of this method, we performed in vivo experiments on exposed rat brain. The estimated images of the absorption coefficients were dominated by the spectral characteristics of hemoglobin. The estimated spectral images of the reduced scattering coefficients had a broad scattering spectrum, exhibiting a larger magnitude at shorter wavelengths, corresponding to the typical spectrum of brain tissue published in the literature. The changes in the estimated absorption and scattering parameters during normoxia, hyperoxia, and anoxia indicate the potential applicability of the method by which to evaluate the pathophysiological conditions of in vivo brain due to the loss of tissue viability.

  18. Multispectral imaging of absorption and scattering properties of in vivo exposed rat brain using a digital red-green-blue camera.

    PubMed

    Yoshida, Keiichiro; Nishidate, Izumi; Ishizuka, Tomohiro; Kawauchi, Satoko; Sato, Shunichi; Sato, Manabu

    2015-05-01

    In order to estimate multispectral images of the absorption and scattering properties in the cerebral cortex of in vivo rat brain, we investigated spectral reflectance images estimated by the Wiener estimation method using a digital RGB camera. A Monte Carlo simulation-based multiple regression analysis for the corresponding spectral absorbance images at nine wavelengths (500, 520, 540, 560, 570, 580, 600, 730, and 760 nm) was then used to specify the absorption and scattering parameters of brain tissue. In this analysis, the concentrations of oxygenated hemoglobin and that of deoxygenated hemoglobin were estimated as the absorption parameters, whereas the coefficient a and the exponent b of the reduced scattering coefficient spectrum approximated by a power law function were estimated as the scattering parameters. The spectra of absorption and reduced scattering coefficients were reconstructed from the absorption and scattering parameters, and the spectral images of absorption and reduced scattering coefficients were then estimated. In order to confirm the feasibility of this method, we performed in vivo experiments on exposed rat brain. The estimated images of the absorption coefficients were dominated by the spectral characteristics of hemoglobin. The estimated spectral images of the reduced scattering coefficients had a broad scattering spectrum, exhibiting a larger magnitude at shorter wavelengths, corresponding to the typical spectrum of brain tissue published in the literature. The changes in the estimated absorption and scattering parameters during normoxia, hyperoxia, and anoxia indicate the potential applicability of the method by which to evaluate the pathophysiological conditions of in vivo brain due to the loss of tissue viability.

  19. Focal plane scanner with reciprocating spatial window

    NASA Technical Reports Server (NTRS)

    Mao, Chengye (Inventor)

    2000-01-01

    A focal plane scanner having a front objective lens, a spatial window for selectively passing a portion of the image therethrough, and a CCD array for receiving the passed portion of the image. All embodiments have a common feature whereby the spatial window and CCD array are mounted for simultaneous relative reciprocating movement with respect to the front objective lens, and the spatial window is mounted within the focal plane of the front objective. In a first embodiment, the spatial window is a slit and the CCD array is one-dimensional, and successive rows of the image in the focal plane of the front objective lens are passed to the CCD array by an image relay lens interposed between the slit and the CCD array. In a second embodiment, the spatial window is a slit, the CCD array is two-dimensional, and a prism-grating-prism optical spectrometer is interposed between the slit and the CCD array so as to cause the scanned row to be split into a plurality of spectral separations onto the CCD array. In a third embodiment, the CCD array is two-dimensional and the spatial window is a rectangular linear variable filter (LVF) window, so as to cause the scanned rows impinging on the LVF to be bandpass filtered into spectral components onto the CCD array through an image relay lens interposed between the LVF and the CCD array.

  20. Noncontact imaging of plethysmographic pulsation and spontaneous low-frequency oscillation in skin perfusion with a digital red-green-blue camera

    NASA Astrophysics Data System (ADS)

    Nishidate, Izumi; Hoshi, Akira; Aoki, Yuta; Nakano, Kazuya; Niizeki, Kyuichi; Aizu, Yoshihisa

    2016-03-01

    A non-contact imaging method with a digital RGB camera is proposed to evaluate plethysmogram and spontaneous lowfrequency oscillation. In vivo experiments with human skin during mental stress induced by the Stroop color-word test demonstrated the feasibility of the method to evaluate the activities of autonomic nervous systems.

  1. Atypical cellular blue nevus or malignant blue nevus?*

    PubMed Central

    Daltro, Luise Ribeiro; Yaegashi, Lygia Bertalha; Freitas, Rodrigo Abdalah; Fantini, Bruno de Carvalho; Souza, Cacilda da Silva

    2017-01-01

    Blue nevus is a benign melanocytic lesion whose most frequent variants are dendritic (common) blue nevus and cellular blue nevus. Atypical cellular blue nevus presents an intermediate histopathology between the typical and a rare variant of malignant blue nevus/melanoma arising in a cellular blue nevus. An 8-year-old child presented a pigmented lesion in the buttock since birth, but with progressive growth in the last two years. After surgical excision, histopathological examination revealed atypical cellular blue nevus. Presence of mitoses, ulceration, infiltration, cytological atypia or necrosis may occur in atypical cellular blue nevus, making it difficult to differentiate it from melanoma. The growth of blue nevus is unusual and considered of high-risk for malignancy, being an indicator for complete resection and periodic follow-up of these patients. PMID:28225968

  2. Bioinspired Polarization Imaging Sensors: From Circuits and Optics to Signal Processing Algorithms and Biomedical Applications: Analysis at the focal plane emulates nature's method in sensors to image and diagnose with polarized light.

    PubMed

    York, Timothy; Powell, Samuel B; Gao, Shengkui; Kahan, Lindsey; Charanya, Tauseef; Saha, Debajit; Roberts, Nicholas W; Cronin, Thomas W; Marshall, Justin; Achilefu, Samuel; Lake, Spencer P; Raman, Baranidharan; Gruev, Viktor

    2014-10-01

    In this paper, we present recent work on bioinspired polarization imaging sensors and their applications in biomedicine. In particular, we focus on three different aspects of these sensors. First, we describe the electro-optical challenges in realizing a bioinspired polarization imager, and in particular, we provide a detailed description of a recent low-power complementary metal-oxide-semiconductor (CMOS) polarization imager. Second, we focus on signal processing algorithms tailored for this new class of bioinspired polarization imaging sensors, such as calibration and interpolation. Third, the emergence of these sensors has enabled rapid progress in characterizing polarization signals and environmental parameters in nature, as well as several biomedical areas, such as label-free optical neural recording, dynamic tissue strength analysis, and early diagnosis of flat cancerous lesions in a murine colorectal tumor model. We highlight results obtained from these three areas and discuss future applications for these sensors.

  3. Stabilized dispersive focal plane systems for space

    NASA Astrophysics Data System (ADS)

    Roming, Peter W. A.; Bayless, Amanda J.; Beebe, Chip R.; Brooks, Mark J.; Davis, Michael W.; Klar, Robert A.; Roberts, John M.; Rose, Randall J.; Winters, Gregory S.

    2012-09-01

    As the costs of space missions continue to rise, the demand for compact, low mass, low-cost technologies that maintain high reliability and facilitate high performance is increasing. One such technology is the stabilized dispersive focal plane system (SDFPS). This technology provides image stabilization while simultaneously delivering spectroscopic or direct imaging functionality using only a single optical path and detector. Typical systems require multiple expensive optical trains and/or detectors, sometimes at the expense of photon throughput. The SDFPS is ideal for performing wide-field low-resolution space-based spectroscopic and direct-imaging surveys. In preparation for a suborbital flight, we have built and ground tested a prototype SDFPS that will concurrently eliminate unwanted image blurring due to the lack of adequate platform stability, while producing images in both spectroscopic and direct-imaging modes. We present the overall design, testing results, and potential scientific applications.

  4. Achromatic phase shifting focal plane masks

    NASA Astrophysics Data System (ADS)

    Newman, Kevin

    The search for life on other worlds is an exciting scientific endeavor that could change the way we perceive our place in the universe. Thousands of extrasolar planets have been discovered using indirect detection techniques. One of the most promising methods for discovering new exoplanets and searching for life is direct imaging with a coronagraph. Exoplanet coronagraphy of Earth-like planets is a challenging task, but we have developed many of the tools necessary to make it feasible. The Phase-Induced Amplitude Apodization (PIAA) Coronagraph is one of the highest-performing architectures for direct exoplanet imaging. With a complex phase-shifting focal plane mask, the PIAA Complex Mask Coronagraph (PIAACMC) can approach the theoretical performance limit for any direct detection technique. The architecture design is flexible enough to be applied to any arbitrary aperture shape, including segmented and obscured apertures. This is an important feature for compatibility with next-generation ground and space-based telescopes. PIAA and PIAACMC focal plane masks have been demonstrated in monochromatic light. An important next step for high-performance coronagraphy is the development of broadband phase-shifting focal plane masks. In this dissertation, we present an algorithm for designing the PIAA and PIAACMC focal plane masks to operate in broadband. We also demonstrate manufacturing of the focal plane masks, and show laboratory results. We use simulations to show the potential performance of the coronagraph system, and the use of wavefront control to correct for mask manufacturing errors. Given the laboratory results and simulations, we show new areas of exoplanet science that can potentially be explored using coronagraph technology. The main conclusion of this dissertation is that we now have the tools required to design and manufacture PIAA and PIAACMC achromatic focal plane masks. These tools can be applied to current and future telescope systems to enable new

  5. Restoring Aperture Profile At Sample Plane

    SciTech Connect

    Jackson, J L; Hackel, R P; Lungershausen, A W

    2003-08-03

    Off-line conditioning of full-size optics for the National Ignition Facility required a beam delivery system to allow conditioning lasers to rapidly raster scan samples while achieving several technical goals. The main purpose of the optical system designed was to reconstruct at the sample plane the flat beam profile found at the laser aperture with significant reductions in beam wander to improve scan times. Another design goal was the ability to vary the beam size at the sample to scan at different fluences while utilizing all of the laser power and minimizing processing time. An optical solution was developed using commercial off-the-shelf lenses. The system incorporates a six meter relay telescope and two sets of focusing optics. The spacing of the focusing optics is changed to allow the fluence on the sample to vary from 2 to 14 Joules per square centimeter in discrete steps. More importantly, these optics use the special properties of image relaying to image the aperture plane onto the sample to form a pupil relay with a beam profile corresponding almost exactly to the flat profile found at the aperture. A flat beam profile speeds scanning by providing a uniform intensity across a larger area on the sample. The relayed pupil plane is more stable with regards to jitter and beam wander. Image relaying also reduces other perturbations from diffraction, scatter, and focus conditions. Image relaying, laser conditioning, and the optical system designed to accomplish the stated goals are discussed.

  6. The Aerodynamic Plane Table

    NASA Technical Reports Server (NTRS)

    Zahm, A F

    1924-01-01

    This report gives the description and the use of a specially designed aerodynamic plane table. For the accurate and expeditious geometrical measurement of models in an aerodynamic laboratory, and for miscellaneous truing operations, there is frequent need for a specially equipped plan table. For example, one may have to measure truly to 0.001 inch the offsets of an airfoil at many parts of its surface. Or the offsets of a strut, airship hull, or other carefully formed figure may require exact calipering. Again, a complete airplane model may have to be adjusted for correct incidence at all parts of its surfaces or verified in those parts for conformance to specifications. Such work, if but occasional, may be done on a planing or milling machine; but if frequent, justifies the provision of a special table. For this reason it was found desirable in 1918 to make the table described in this report and to equip it with such gauges and measures as the work should require.

  7. Study of the nucleation and growth of antibiotic labeled Au NPs and blue luminescent Au8 quantum clusters for Hg2+ ion sensing, cellular imaging and antibacterial applications

    NASA Astrophysics Data System (ADS)

    Khandelwal, Puneet; Singh, Dheeraj K.; Sadhu, Subha; Poddar, Pankaj

    2015-11-01

    Herein, we report a detailed experimental study supported by DFT calculations to understand the mechanism behind the synthesis of cefradine (CFD - an antibiotic) labeled gold nanoparticles (Au NPs) by employing CFD as both a mild reducing and capping agent. The analysis of the effect of growth conditions reveals that a higher concentration of HAuCl4 results in the formation of an increasing fraction of anisotropic structures, higher temperature leads to the formation of quasi-spherical particles instead of anisotropic ones, and larger pH leads to the formation of much smaller particles. The cyclic voltammetry (CV) results show that when the pH of the reaction medium increases from 4 to 6, the reduction potential of CFD increases which leads to the synthesis of nanoparticles (in a pH 4 reaction) to quantum clusters (in a pH 6 reaction). The MALDI-TOF mass spectrometry results of supernatant of the pH 6 reaction indicate the formation of [Au8(CFD)2S6] QCs which show fluorescence at ca. 432 nm with a Stokes shift of ca. 95 nm. The blue luminescence from Au8 QCs was applied for sensing of Hg2+ ions on the basis of an aggregation-induced fluorescence quenching mechanism and offers good selectivity and a high sensitivity with a limit of detection ca. 2 nM which is lower than the detection requirement of 10 nM by the U.S. EPA and 30 nM by WHO for drinking water. We have also applied the sensing probe to detect Hg2+ ions in bacterial samples. Further, we have investigated the antibacterial property of as-synthesized Au NPs using MIC, growth curve and cell survival assay. The results show that Au NPs could reduce the cell survival very efficiently rather than the cell growth in comparison to the antibiotic itself. The scanning electron microscopy study shows the degradation and blebbing of the bacterial cell wall upon exposure with Au NPs which was further supported by fluorescence microscopy results. These Au NPs did not show reactive oxygen species generation. We believe

  8. Astronomical imaging by pupil plane interferometry

    NASA Technical Reports Server (NTRS)

    Ribak, Erez

    1989-01-01

    Comparing rotational shear interferometry to standard speckle interferometry, it is found that it is easier in the first case to separate the atmospheric phases from the object transform phases. Phase closure and blind deconvolution should be directly applicable. Laboratory simulations were conducted to verify theoretical predictions and computer simulations for the phase closure case, and preliminary results show promise.

  9. MTI Focal Plane Assembly Design and Performance

    SciTech Connect

    Ballard, M.; Rienstra, J.L.

    1999-06-17

    The focal plane assembly for the Multispectral Thermal Imager (MTI) consists of sensor chip assemblies, optical filters, and a vacuum enclosure. Sensor chip assemblies, composed of linear detector arrays and readout integrated circuits, provide spatial resolution in the cross-track direction for the pushbroom imager. Optical filters define 15 spectral bands in a range from 0.45 {micro}m to 10.7 {micro}m. All the detector arrays are mounted on a single focal plane and are designed to operate at 75 K. Three pairs of sensor chip assemblies (SCAs) are required to provide cross-track coverage in all 15 spectral bands. Each pair of SCAs includes detector arrays made from silicon, iridium antimonide, and mercury cadmium telluride. Read out integrated circuits multiplex the signals from the detectors to 18 separate video channels. Optical filter assemblies defining the spectral bands are mounted over the linear detector arrays. Each filter assembly consists of several filter strips bonded together side-by-side. The MTI focal plane assembly has been integrated with the rest of the payload and has undergone detailed testing and calibration. This paper includes representative test data for the various spectral bands and the overall performance of the focal plane assembly.

  10. Techniques for identifying dust devils in mars pathfinder images

    USGS Publications Warehouse

    Metzger, S.M.; Carr, J.R.; Johnson, J. R.; Parker, T.J.; Lemmon, M.T.

    2000-01-01

    Image processing methods used to identify and enhance dust devil features imaged by IMP (Imager for Mars Pathfinder) are reviewed. Spectral differences, visible red minus visible blue, were used for initial dust devil searches, driven by the observation that Martian dust has high red and low blue reflectance. The Martian sky proved to be more heavily dust-laden than pre-Pathfinder predictions, based on analysis of images from the Hubble Space Telescope. As a result, these initial spectral difference methods failed to contrast dust devils with background dust haze. Imager artifacts (dust motes on the camera lens, flat-field effects caused by imperfections in the CCD, and projection onto a flat sensor plane by a convex lens) further impeded the ability to resolve subtle dust devil features. Consequently, reference images containing sky with a minimal horizon were first subtracted from each spectral filter image to remove camera artifacts and reduce the background dust haze signal. Once the sky-flat preprocessing step was completed, the red-minus-blue spectral difference scheme was attempted again. Dust devils then were successfully identified as bright plumes. False-color ratios using calibrated IMP images were found useful for visualizing dust plumes, verifying initial discoveries as vortex-like features. Enhancement of monochromatic (especially blue filter) images revealed dust devils as silhouettes against brighter background sky. Experiments with principal components transformation identified dust devils in raw, uncalibrated IMP images and further showed relative movement of dust devils across the Martian surface. A variety of methods therefore served qualitative and quantitative goals for dust plume identification and analysis in an environment where such features are obscure.

  11. NASA Connect: 'Plane Weather'

    NASA Technical Reports Server (NTRS)

    1999-01-01

    Excerpt from the NASA Connect show 'Plane Weather' This clip explains what high and low pressure weather systems are, and how they form. Weather affects our daily lives. The elements of weather: rain, wind, fog, ice and snow affect the operation and flight of an airplane. In this program, NASA and FAA researchers will introduce students to math, science, and weather; demonstrate how these elements influence flight; and show how NASA and FAA research is used to limit the effects of these elements on flight. Students will examine: the tools, techniques, and technologies used by engineers and scientists to detect these and other climatological factors affecting aircraft in flight. The lesson and classroom experiment will involve students in the scientific process and emphasizing problem solving, measurement, and reasoning skills.

  12. NASA Connect: 'Plane Weather'

    NASA Technical Reports Server (NTRS)

    1999-01-01

    Excerpt from the NASA Connect show 'Plane Weather' This clip explains what high and low pressure weather systems are, and how these affect weather patterns. Weather affects our daily lives. The elements of weather: rain, wind, fog, ice and snow affect the operation and flight of an airplane. In this program, NASA and FAA researchers will introduce students to math, science, and weather; demonstrate how these elements influence flight; and show how NASA and FAA research is used to limit the effects of these elements on flight. Students will examine: the tools, techniques, and technologies used by engineers and scientists to detect these and other climatological factors affecting aircraft in flight. The lesson and classroom experiment will involve students in the scientific process and emphasizing problem solving, measurement, and reasoning skills.

  13. Phase measurement profilometry based on a virtual reference plane method

    NASA Astrophysics Data System (ADS)

    Ren, Hongbing; Lee, Jinlong; Gao, Xiaorong

    2016-09-01

    In Phase Measurement Profilometry(PMP), the setting of the reference plane plays an important role. It is a critical step to capture the grating fringe projected onto the reference plane in PMP. However, it is sometimes difficult to choose and place the reference plane in practical applications. In this paper, a virtual reference plane is introduced into PMP, with which 3D measurement can be realized without using the physical reference plane. The virtual reference plane is generated through extracting a partial area of the deformed fringe image that corresponds to a planar region and employing the interpolation algorithm. The method is proved theoretically through simulation experiments, providing a new suggestion for actual measurement by PMP.

  14. Blue Fermi flat spectrum radio quasars

    NASA Astrophysics Data System (ADS)

    Ghisellini, G.; Tavecchio, F.; Foschini, L.; Sbarrato, T.; Ghirlanda, G.; Maraschi, L.

    2012-09-01

    Many blazars detected by the Fermi satellite, observed spectroscopically in the optical, are line-less, and have been classified as BL Lac objects. Optical-ultraviolet (UV) photometry of nearly 100 of them allowed us to determine the redshift for a handful of objects and redshift upper limits in the great majority. A few of these are candidates to be 'blue quasars', namely flat spectrum radio quasars whose broad emission lines are hidden by an overwhelming synchrotron emission peaking in the UV. This implies that the emitting electrons have high energies. In turn, this requires relatively weak radiative cooling, a condition that can be met if the main radiative dissipation of the jet power occurs outside the broad-line region. We confirm this hypothesis by studying and modelling the spectral energy distributions of the four 'blue quasars' recently discovered. Furthermore, we discuss the distribution of Fermi blazars in the γ-ray spectral index-γ-ray luminosity plane, and argue that 'blue quasars' objects are a minority within the blazar populations.

  15. [Blue light and eye health].

    PubMed

    Zou, Leilei; Dai, Jinhui

    2015-01-01

    Blue light, with the wavelength between 400 nm and 500 nm, has caused public concern because of the injury to the retinal cells. Meanwhile, it is important in circadian rhythm regulation, scotopic vision and ocular growth. Is the blue light safe? Should it be eliminated from the daily life? Here we review the effect and safety of the blue light.

  16. Learning the Blues. [Lesson Plan].

    ERIC Educational Resources Information Center

    2001

    This lesson introduces students to the "blues," one of the most distinctive and influential elements of African-American musical tradition. With this lesson plan, students can take a virtual field trip to Memphis, Tennessee, one of the prominent centers of blues activities, and explore the history of the blues in the work of W. C. Handy…

  17. Materials, devices, techniques, and applications for Z-plane focal plane array technology II; Proceedings of the Meeting, San Diego, CA, July 12, 13, 1990

    NASA Astrophysics Data System (ADS)

    Carson, John C.

    1990-11-01

    Various papers on materials, devices, techniques, and applications for X-plane focal plane array technology are presented. Individual topics addressed include: application of Z-plane technology to the remote sensing of the earth from GEO, applications of smart neuromorphic focal planes, image-processing of Z-plane technology, neural network Z-plane implementation with very high interconnection rates, using a small IR surveillance satellite for tactical applications, establishing requirements for homing applications, Z-plane technology. Also discussed are: on-array spike suppression signal processing, algorithms for on-focal-plane gamma circumvention and time-delay integration, current HYMOSS Z-technology, packaging of electrons for on- and off-FPA signal processing, space/performance qualification of tape automated bonded devices, automation in tape automated bonding, high-speed/high-volume radiometric testing of Z-technology focal planes, 128-layer HYMOSS-module fabrication issues, automation of IRFPA production processes.

  18. The Blue Emu

    NASA Technical Reports Server (NTRS)

    Descalzi, Doug; Gillett, John; Gordon, Carlton; Keener, ED; Novak, Ken; Puente, Laura

    1993-01-01

    The primary goal in designing the Blue Emu was to provide an airline with a cost efficient and profitable means of transporting passengers between the major cities in Aeroworld. The design attacks the market where a demand for inexpensive transportation exists and for this reason the Blue Emu is an attractive investment for any airline. In order to provide a profitable aircraft, special attention was paid to cost and economics. For example, in manufacturing, simplicity was stressed in structural design to reduce construction time and cost. Aerodynamic design employed a tapered wing which reduced the induced drag coefficient while also reducing the weight of the wing. Even the propulsion system was selected with cost effectiveness in mind, yet also to maintain the marketability of the aircraft. Thus, in every aspect of the design, consideration was given to economics and marketability of the final product.

  19. Blue metal-poor stars

    NASA Astrophysics Data System (ADS)

    Preston, George W.; Sneden, Christopher

    2004-12-01

    We review the discovery of blue metal-poor (BMP) stars and the resolution of this population into blue stragglers and intermediate-age Main-Sequence stars by use of binary fractions. We show that the specific frequencies of blue stragglers in the halo field and in globular clusters differ by an order of magnitude. We attribute this difference to the different modes of production of these two populations. We report carbon and s-process enrichment among very metal-poor field blue stragglers and discuss how this result can be used to further resolve field blue stragglers into groups formed during RGB and AGB evolution of their erstwhile primary companions.

  20. A Panel of Trypanosoma brucei Strains Tagged with Blue and Red-Shifted Luciferases for Bioluminescent Imaging in Murine Infection Models

    PubMed Central

    Van Reet, Nick; Van de Vyver, Hélène; Pyana, Patient Pati; Van der Linden, Anne Marie; Büscher, Philippe

    2014-01-01

    Background Genetic engineering with luciferase reporter genes allows monitoring Trypanosoma brucei (T.b.) infections in mice by in vivo bioluminescence imaging (BLI). Until recently, luminescent T.b. models were based on Renilla luciferase (RLuc) activity. Our study aimed at evaluating red-shifted luciferases for in vivo BLI in a set of diverse T.b. strains of all three subspecies, including some recently isolated from human patients. Methodology/Principal findings We transfected T.b. brucei, T.b. rhodesiense and T.b. gambiense strains with either RLuc, click beetle red (CBR) or Photinus pyralis RE9 (PpyRE9) luciferase and characterised their in vitro luciferase activity, growth profile and drug sensitivity, and their potential for in vivo BLI. Compared to RLuc, the red-shifted luciferases, CBR and PpyRE9, allow tracking of T.b. brucei AnTaR 1 trypanosomes with higher details on tissue distribution, and PpyRE9 allows detection of the parasites with a sensitivity of at least one order of magnitude higher than CBR luciferase. With CBR-tagged T.b. gambiense LiTaR1, T.b. rhodesiense RUMPHI and T.b. gambiense 348 BT in an acute, subacute and chronic infection model respectively, we observed differences in parasite tropism for murine tissues during in vivo BLI. Ex vivo BLI on the brain confirmed central nervous system infection by all luminescent strains of T.b. brucei AnTaR 1, T.b. rhodesiense RUMPHI and T.b. gambiense 348 BT. Conclusions/Significance We established a genetically and phenotypically diverse collection of bioluminescent T.b. brucei, T.b. gambiense and T.b. rhodesiense strains, including drug resistant strains. For in vivo BLI monitoring of murine infections, we recommend trypanosome strains transfected with red-shifted luciferase reporter genes, such as CBR and PpyRE9. Red-shifted luciferases can be detected with a higher sensitivity in vivo and at the same time they improve the spatial resolution of the parasites in the entire body due to the better

  1. Platinum blue staining of cells grown in electrospun scaffolds.

    PubMed

    Yusuf, Mohammed; Millas, Ana Luiza G; Estandarte, Ana Katrina C; Bhella, Gurdeep K; McKean, Robert; Bittencourt, Edison; Robinson, Ian K

    2014-01-01

    Fibroblast cells grown in electrospun polymer scaffolds were stained with platinum blue, a heavy metal stain, and imaged using scanning electron microscopy. Good contrast on the cells was achieved compared with samples that were gold sputter coated. The cell morphology could be clearly observed, and the cells could be distinguished from the scaffold fibers. Here we optimized the required concentration of platinum blue for imaging cells grown in scaffolds and show that a higher concentration causes platinum aggregation. Overall, platinum blue is a useful stain for imaging cells because of its enhanced contrast using scanning electron microscopy (SEM). In the future it would be useful to investigate cell growth and morphology using three-dimensional imaging methods.

  2. Blue upconversion thulium laser

    NASA Astrophysics Data System (ADS)

    Nguyen, Dinh C.; Faulkner, George E.; Weber, Michael E.; Dulick, Michael

    1990-04-01

    We report a blue emission upconversion solid-state laser based on Tm3+:YLF. Under double resonance excitation at 780.8 nm (near-ir) and 648.8 nm (red), the Tm3+ ion is sequentially excited from the 3H6 ground state to the 1D2 excited state through the 3H4 intermediate level. The laser output at 450 and 453 nm corresponds to the 1D2 -> 3F4 transitions of Tm3+ ions in YLF.

  3. Keratoglobus and blue sclera.

    PubMed

    Biglan, A W; Brown, S I; Johnson, B L

    1977-02-01

    Five patients from two families had similar features including keratoglobus, blue scleras, hyperextensibility of the hand, wrist, and ankle joints, sensorineural conduction hearing alterations, and mottling of the teeth. Keratoglobus had been observed in all patients at, or shortly after, birth. Corneal perforations developed in seven of the ten eyes after minimal trauma. Repair of these perforations was complicated by the extremely thin corneas and six eyes had to be either enucleated or eviscerated. Histopathological examination of two of the enucleated eyes showed the corneal stromas of both eyes to be estremely thin, Bowman's membrane was absent, and Descemet's membrane was unusually thick. This condition has an autosomal-recessive inheritance pattern.

  4. Root planing with interdental papilla reflection and fiber optic illumination.

    PubMed

    Reinhardt, R A; Johnson, G K; Tussing, G J

    1985-12-01

    The complete removal of accretions during closed scaling and root planing in moderate-deep pockets is difficult, presumably due to inadequate mechanical and visual access. The purpose of this study was to evaluate the effect of minimal papilla reflection and illumination with a prototype fiber optic unit on root planing efficiency. Nonmolar teeth with moderate-deep interproximal pockets (greater than 3 mm) in four patients scheduled to receive immediate complete dentures were randomly divided into groups for treatment: Group I--interproximal root planing augmented by papilla reflection and fiber optic illumination (n = 26 surfaces); Group II--interproximal root planing with papilla reflection only (n = 24); Group III--untreated controls (n = 23). Immediately after treatment, the experimental teeth were extracted, stained with toluidine blue and interproximal areas were evaluated for remaining accretions with a microscope-digitizing pad-computer system. Significantly less (P less than 0.01) root surface was covered by deposits in Group I than Group II (0.57 +/- 0.29% vs. 2.42 +/- 0.63%), and both treatment groups had fewer (P less than 0.0005) accretions than untreated controls (57.72 +/- 3.40%). These results suggest that root planing with papilla reflection produces an interproximal surface with few remaining deposits, and fiber optic illumination and transillumination further enhance this effect.

  5. Stereo Imaging Miniature Endoscope

    NASA Technical Reports Server (NTRS)

    Bae, Youngsam; Manohara, Harish; White, Victor; Shcheglov, Kirill V.; Shahinian, Hrayr

    2011-01-01

    Stereo imaging requires two different perspectives of the same object and, traditionally, a pair of side-by-side cameras would be used but are not feasible for something as tiny as a less than 4-mm-diameter endoscope that could be used for minimally invasive surgeries or geoexploration through tiny fissures or bores. The proposed solution here is to employ a single lens, and a pair of conjugated, multiple-bandpass filters (CMBFs) to separate stereo images. When a CMBF is placed in front of each of the stereo channels, only one wavelength of the visible spectrum that falls within the passbands of the CMBF is transmitted through at a time when illuminated. Because the passbands are conjugated, only one of the two channels will see a particular wavelength. These time-multiplexed images are then mixed and reconstructed to display as stereo images. The basic principle of stereo imaging involves an object that is illuminated at specific wavelengths, and a range of illumination wavelengths is time multiplexed. The light reflected from the object selectively passes through one of the two CMBFs integrated with two pupils separated by a baseline distance, and is focused onto the imaging plane through an objective lens. The passband range of CMBFs and the illumination wavelengths are synchronized such that each of the CMBFs allows transmission of only the alternate illumination wavelength bands. And the transmission bandwidths of CMBFs are complementary to each other, so that when one transmits, the other one blocks. This can be clearly understood if the wavelength bands are divided broadly into red, green, and blue, then the illumination wavelengths contain two bands in red (R1, R2), two bands in green (G1, G2), and two bands in blue (B1, B2). Therefore, when the objective is illuminated by R1, the reflected light enters through only the left-CMBF as the R1 band corresponds to the transmission window of the left CMBF at the left pupil. This is blocked by the right CMBF. The

  6. Blue ocean leadership.

    PubMed

    Kim, W Chan; Mauborgne, Renée

    2014-05-01

    Ten years ago, two INSEAD professors broke ground by introducing "blue ocean strategy," a new model for discovering uncontested markets that are ripe for growth. In this article, they apply their concepts and tools to what is perhaps the greatest challenge of leadership: closing the gulf between the potential and the realized talent and energy of employees. Research indicates that this gulf is vast: According to Gallup, 70% of workers are disengaged from their jobs. If companies could find a way to convert them into engaged employees, the results could be transformative. The trouble is, managers lack a clear understanding of what changes they could make to bring out the best in everyone. Here, Kim and Mauborgne offer a solution to that problem: a systematic approach to uncovering, at each level of the organization, which leadership acts and activities will inspire employees to give their all, and a process for getting managers throughout the company to start doing them. Blue ocean leadership works because the managers' "customers"-that is, the people managers oversee and report to-are involved in identifying what's effective and what isn't. Moreover, the approach doesn't require leaders to alter who they are, just to undertake a different set of tasks. And that kind of change is much easier to implement and track than changes to values and mind-sets.

  7. Design study of the accessible focal plane telescope for shuttle

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The design and cost analysis of an accessible focal plane telescope for Spacelab is presented in blueprints, tables, and graphs. Topics covered include the telescope tube, the telescope mounting, the airlock plus Spacelab module aft plate, the instrument adapter, and the instrument package. The system allows access to the image plane with instrumentation that can be operated by a scientist in a shirt sleeve environment inside a Spacelab module.

  8. Active point out-of-plane ultrasound calibration

    NASA Astrophysics Data System (ADS)

    Cheng, Alexis; Guo, Xiaoyu; Zhang, Haichong K.; Kang, Hyunjae; Etienne-Cummings, Ralph; Boctor, Emad M.

    2015-03-01

    Image-guided surgery systems are often used to provide surgeons with informational support. Due to several unique advantages such as ease of use, real-time image acquisition, and no ionizing radiation, ultrasound is a common intraoperative medical imaging modality used in image-guided surgery systems. To perform advanced forms of guidance with ultrasound, such as virtual image overlays or automated robotic actuation, an ultrasound calibration process must be performed. This process recovers the rigid body transformation between a tracked marker attached to the transducer and the ultrasound image. Point-based phantoms are considered to be accurate, but their calibration framework assumes that the point is in the image plane. In this work, we present the use of an active point phantom and a calibration framework that accounts for the elevational uncertainty of the point. Given the lateral and axial position of the point in the ultrasound image, we approximate a circle in the axial-elevational plane with a radius equal to the axial position. The standard approach transforms all of the imaged points to be a single physical point. In our approach, we minimize the distances between the circular subsets of each image, with them ideally intersecting at a single point. We simulated in noiseless and noisy cases, presenting results on out-of-plane estimation errors, calibration estimation errors, and point reconstruction precision. We also performed an experiment using a robot arm as the tracker, resulting in a point reconstruction precision of 0.64mm.

  9. Wafer plane inspection with soft resist thresholding

    NASA Astrophysics Data System (ADS)

    Hess, Carl; Shi, Rui-fang; Wihl, Mark; Xiong, Yalin; Pang, Song

    2008-10-01

    Wafer Plane Inspection (WPI) is an inspection mode on the KLA-Tencor TeraScaTM platform that uses the high signalto- noise ratio images from the high numerical aperture microscope, and then models the entire lithographic process to enable defect detection on the wafer plane[1]. This technology meets the needs of some advanced mask manufacturers to identify the lithographically-significant defects while ignoring the other non-lithographically-significant defects. WPI accomplishes this goal by performing defect detection based on a modeled image of how the mask features would actually print in the photoresist. There are several advantages to this approach: (1) the high fidelity of the images provide a sensitivity advantage over competing approaches; (2) the ability to perform defect detection on the wafer plane allows one to only see those defects that have a printing impact on the wafer; (3) the use of modeling on the lithographic portion of the flow enables unprecedented flexibility to support arbitrary illumination profiles, process-window inspection in unit time, and combination modes to find both printing and non-printing defects. WPI is proving to be a valuable addition to the KLA-Tencor detection algorithm suite. The modeling portion of WPI uses a single resist threshold as the final step in the processing. This has been shown to be adequate on several advanced customer layers, but is not ideal for all layers. Actual resist chemistry has complicated processes including acid and base-diffusion and quench that are not consistently well-modeled with a single resist threshold. We have considered the use of an advanced resist model for WPI, but rejected it because the burdensome requirements for the calibration of the model were not practical for reticle inspection. This paper describes an alternative approach that allows for a "soft" resist threshold to be applied that provides a more robust solution for the most challenging processes. This approach is just

  10. Hyperspectral Fluorescence and Reflectance Imaging Instrument

    NASA Technical Reports Server (NTRS)

    Ryan, Robert E.; O'Neal, S. Duane; Lanoue, Mark; Russell, Jeffrey

    2008-01-01

    The system is a single hyperspectral imaging instrument that has the unique capability to acquire both fluorescence and reflectance high-spatial-resolution data that is inherently spatially and spectrally registered. Potential uses of this instrument include plant stress monitoring, counterfeit document detection, biomedical imaging, forensic imaging, and general materials identification. Until now, reflectance and fluorescence spectral imaging have been performed by separate instruments. Neither a reflectance spectral image nor a fluorescence spectral image alone yields as much information about a target surface as does a combination of the two modalities. Before this system was developed, to benefit from this combination, analysts needed to perform time-consuming post-processing efforts to co-register the reflective and fluorescence information. With this instrument, the inherent spatial and spectral registration of the reflectance and fluorescence images minimizes the need for this post-processing step. The main challenge for this technology is to detect the fluorescence signal in the presence of a much stronger reflectance signal. To meet this challenge, the instrument modulates artificial light sources from ultraviolet through the visible to the near-infrared part of the spectrum; in this way, both the reflective and fluorescence signals can be measured through differencing processes to optimize fluorescence and reflectance spectra as needed. The main functional components of the instrument are a hyperspectral imager, an illumination system, and an image-plane scanner. The hyperspectral imager is a one-dimensional (line) imaging spectrometer that includes a spectrally dispersive element and a two-dimensional focal plane detector array. The spectral range of the current imaging spectrometer is between 400 to 1,000 nm, and the wavelength resolution is approximately 3 nm. The illumination system consists of narrowband blue, ultraviolet, and other discrete

  11. Characterization of DECam focal plane detectors

    SciTech Connect

    Diehl, H.Thomas; Angstadt, Robert; Campa, Julia; Cease, Herman; Derylo, Greg; Emes, John H.; Estrada, Juan; Kibik, Donna; Flaugher, Brenna L.; Holland, Steve E.; Jonas, Michelle; /Fermilab /Madrid, CIEMAT /LBL, Berkeley /Argonne /Pennsylvania U.

    2008-06-01

    DECam is a 520 Mpix, 3 square-deg FOV imager being built for the Blanco 4m Telescope at CTIO. This facility instrument will be used for the 'Dark Energy Survey' of the southern galactic cap. DECam has chosen 250 ?m thick CCDs, developed at LBNL, with good QE in the near IR for the focal plane. In this work we present the characterization of these detectors done by the DES team, and compare it to the DECam technical requirements. The results demonstrate that the detectors satisfy the needs for instrument.

  12. Adolescent Development: Body Blues.

    ERIC Educational Resources Information Center

    Yates, Alayne; Brodkin, Adele M.

    1994-01-01

    When early adolescents equate body image with self-image, they risk eating and exercise disorders, with dangerous results. Interviews with two experts present information to help middle school teachers understand the problem and intervene with students whose preoccupation with appearance or prowess can, taken to the extreme, be fatal. (SM)

  13. CCD photometry in the globular cluster NGC 288. I - Blue stragglers and main-sequence binary stars

    NASA Technical Reports Server (NTRS)

    Bolte, Michael

    1992-01-01

    Photometry based on a mosaic of CCD images in B and V is presented for the globular cluster NGC 288. The spatial coverage ranges from the cluster core to about 6 core radii, and stars have been measured over the absolute visual magnitude range -1.2 to 8.4. The cluster is shown to contain a significant number of blue straggler stars in the central regions, and there is an excess of objects brighter and redder than the single-star main-sequence in the color-magnitude plane. These objects are interpreted as a population of main-sequence binary stars. With this interpretation, the explicity measured fraction of binary stars is 10 percent, which sets a lower limit for the total binary population.

  14. National Aerospace Plane (NASP) program

    NASA Technical Reports Server (NTRS)

    1990-01-01

    Artists concept of the X-30 aerospace plane flying through Earth's atmosphere on its way to low-Earth orbit. the experimental concept is part of the National Aero-Space Plane Program. The X-30 is planned to demonstrate the technology for airbreathing space launch and hypersonic cruise vehicles. Photograph and caption published in Winds of Change, 75th Anniversary NASA publication (page 117), by James Schultz.

  15. A multilinear constraint on dichromatic planes for illumination estimation.

    PubMed

    Toro, Javier; Funt, Brian

    2007-01-01

    A new multilinear constraint on the color of the scene illuminant based on the dichromatic reflection model is proposed. The formulation avoids the problem, common to previous dichromatic methods, of having to first identify pixels corresponding to the same surface material. Once pixels from two or more materials have been identified, their corresponding dichromatic planes can be intersected to yield the illuminant color. However, it is not always easy to determine which pixels from an arbitrary region of an image belong to which dichromatic plane. The image region may cover an area of the scene encompassing several different materials and, hence, pixels from several different dichromatic planes. The new multilinear constraint accounts for this multiplicity of materials and provides a mechanism for choosing the most plausible illuminant from a finite set of candidate illuminants. The performance of this new method is tested on a database of real images.

  16. Jupiter in blue, ultraviolet and near infrared

    NASA Technical Reports Server (NTRS)

    2000-01-01

    These three images of Jupiter, taken through the narrow angle camera of NASA's Cassini spacecraft from a distance of 77.6 million kilometers (48.2 million miles) on October 8, reveal more than is apparent to the naked eye through a telescope.

    The image on the left was taken through the blue filter. The one in the middle was taken in the ultraviolet. The one on the right was taken in the near infrared.

    The blue-light filter is within the part of the electromagnetic spectrum detectable by the human eye. The appearance of Jupiter in this image is, consequently, very familiar. The Great Red Spot (below and to the right of center) and the planet's well-known banded cloud lanes are obvious. The brighter bands of clouds are called zones and are probably composed of ammonia ice particles. The darker bands are called belts and are made dark by particles of unknown composition intermixed with the ammonia ice.

    Jupiter's appearance changes dramatically in the ultraviolet and near infrared images. These images are near negatives of each other and illustrate the way in which observations in different wavelength regions can reveal different physical regimes on the planet.

    All gases scatter sunlight efficiently at short wavelengths; this is why the sky appears blue on Earth. The effect is even more pronounced in the ultraviolet. The gases in Jupiter's atmosphere, above the clouds, are no different. They scatter strongly in the ultraviolet, making the deep banded cloud layers invisible in the middle image. Only the very high altitude haze appears dark against the bright background. The contrast is reversed in the near infrared, where methane gas, abundant on Jupiter but not on Earth, is strongly absorbing and therefore appears dark. Again the deep clouds are invisible, but now the high altitude haze appears relatively bright against the dark background. High altitude haze is seen over the poles and the equator.

    The Great Red Spot, prominent in all images, is

  17. Sunspot temperatures from red and blue photometry

    NASA Astrophysics Data System (ADS)

    Chapman, G. A.; Cookson, A. M.; Preminger, D. G.

    2011-08-01

    Photometric images are used to measure the temperature of sunspots at different wavelengths. Images at 672.3 nm and 472.3 nm are obtained at the San Fernando Observatory using the CFDT2 (2.5'' x 2.5'' pixels). Images at 607.1 nm and 409.4 nm are obtained by the PSPT at Mauna Loa Observatory. Monochromatic intensities are converted to temperatures as in Steinegger et al (1990). The pixel by pixel temperature for a sunspot is converted into a bolometric contrast for that sunspot according to Chapman et al (1994). Sunspot temperatures, i.e., their bolometric contrasts, are calculated from both red (672.3 nm) and blue wavelengths (472.3 nm) and compared.

  18. Resolution in 3D in multifocal plane microscopy

    NASA Astrophysics Data System (ADS)

    Chao, Jerry; Ram, Sripad; Abraham, Anish V.; Ward, E. Sally; Ober, Raimund J.

    2008-02-01

    Using single molecule microscopy, biological interactions can be imaged and studied at the level of individual biomolecules. When characterizing an imaged biological interaction, the distance separating the two participating biomolecules can provide valuable information. Therefore, the resolvability of an imaging setup is of practical significance in the analysis of the acquired image data. Importantly, the resolvability of the imaging setup needs evaluation in the 3D context, since in general biomolecules reside in 3D space within the cellular environment. We recently introduced an information-theoretic 2D resolution measure which shows that the resolution limit due to Rayleigh's criterion can be overcome. This new result predicts that the resolution of optical microscopes is not limited, but rather can be improved with increased photon counts detected from the single molecules. The 2D result was subsequently extended to the 3D context, and the proposed information-theoretic 3D resolution measure can readily be used to determine the resolvability of a conventional single focal plane imaging setup. Here, we consider the 3D resolution measure for a multifocal plane microscope setup, an imaging system which allows the concurrent imaging of multiple focal planes within a specimen. The technique is useful in applications such as the tracking of subcellular objects in 3D. By comparing their 3D resolution measures, we find a two-plane setup to outperform a comparable conventional single-plane setup in resolvability over a range of axial locations for the single molecule pair. Moreover, we investigate and compare the impact of noise on the resolvability of the two setups.

  19. The blue copper protein gene of Alcaligenes faecalis S-6 directs secretion of blue copper protein from Escherichia coli cells.

    PubMed Central

    Yamamoto, K; Uozumi, T; Beppu, T

    1987-01-01

    The gene encoding a blue copper protein (a member of the pseudoazurins) of 123 amino acid residues, containing a single type I Cu2+ ion, was cloned from Alcaligenes faecalis S-6. The nucleotide sequence of the coding region, as well as the 5'- and 3'-flanking regions, was determined. The deduced amino acid sequence after Glu-24 coincided with the reported sequence of the blue protein, and its NH2-terminal sequence of 23 residues resembled a typical signal peptide. The cloned gene was expressed under the control of the tac promoter in Escherichia coli, and the correctly processed blue protein was secreted into the periplasm. The blue protein produced in E. coli possessed the activity to transfer electrons to the copper-containing nitrite reductase of A. faecalis S-6 in vitro. Images PMID:2824441

  20. The Blue Comet: A Railroad's Astronomical Heritage

    NASA Astrophysics Data System (ADS)

    Rumstay, Kenneth S.

    2009-01-01

    Between 1929 February 21 and 1941 September 27, the Central New Jersey Railroad operated a luxury passenger train between Jersey City and Atlantic City. Named The Blue Comet, the locomotive, tender, and coaches sported a unique royal blue paint scheme designed to evoke images of celestial bodies speeding through space. Inside each car were etched window panes and lampshades featuring stars and comets. And each coach sported the name of a famous comet on its side; these comets were of course named for their discoverers. Some of the astronomers honored in this unique fashion remain famous to this day, or at least their comets do. The names D'Arrest, Barnard, Encke, Faye, Giacobini, Halley, Olbers, Temple, Tuttle, and Westphal are familiar ones. But Biela, Brorsen, deVico, Spitaler, and Winnecke have now largely faded into obscurity; their stories are recounted here. Although more than sixty years have elapsed since its last run, The Blue Comet, perhaps the most famous passenger train in American history, lives on in the memories of millions of passengers and railfans. This famous train returned to the attention of millions of television viewers on the evening of 2007 June 3, in an episode of the HBO series The Sopranos. This work was supported by a faculty development grant from Valdosta State University.

  1. A method of plane geometry primitive presentation

    NASA Astrophysics Data System (ADS)

    Jiao, Anbo; Luo, Haibo; Chang, Zheng; Hui, Bin

    2014-11-01

    Point feature and line feature are basic elements in object feature sets, and they play an important role in object matching and recognition. On one hand, point feature is sensitive to noise; on the other hand, there are usually a huge number of point features in an image, which makes it complex for matching. Line feature includes straight line segment and curve. One difficulty in straight line segment matching is the uncertainty of endpoint location, the other is straight line segment fracture problem or short straight line segments joined to form long straight line segment. While for the curve, in addition to the above problems, there is another difficulty in how to quantitatively describe the shape difference between curves. Due to the problems of point feature and line feature, the robustness and accuracy of target description will be affected; in this case, a method of plane geometry primitive presentation is proposed to describe the significant structure of an object. Firstly, two types of primitives are constructed, they are intersecting line primitive and blob primitive. Secondly, a line segment detector (LSD) is applied to detect line segment, and then intersecting line primitive is extracted. Finally, robustness and accuracy of the plane geometry primitive presentation method is studied. This method has a good ability to obtain structural information of the object, even if there is rotation or scale change of the object in the image. Experimental results verify the robustness and accuracy of this method.

  2. In-plane and out-of-plane tissue micro-displacement measurement by correlation coefficients of optical coherence tomography.

    PubMed

    Kurokawa, Kazuhiro; Makita, Shuichi; Hong, Young-Joo; Yasuno, Yoshiaki

    2015-05-01

    We propose a method to measure the in-plane and out-of-plane displacements of tissue using the correlation coefficients of optical coherence tomography (OCT) signals. The displacements are determined by the local correlation coefficients between digitally shifted reference OCT images and a target image. The method achieves sub-micron displacement measurement with an accuracy better than 0.32 μm and repeatability better than 0.36 μm. The feasibility of the method was examined by measuring the displacement field of a laser irradiated porcine retina. This method successfully visualized the dynamic change of the displacement field during laser irradiation.

  3. Ultrafast vascular strain compounding using plane wave transmission.

    PubMed

    Hansen, H H G; Saris, A E C M; Vaka, N R; Nillesen, M M; de Korte, C L

    2014-03-03

    Deformations of the atherosclerotic vascular wall induced by the pulsating blood can be estimated using ultrasound strain imaging. Because these deformations indirectly provide information on mechanical plaque composition, strain imaging is a promising technique for differentiating between stable and vulnerable atherosclerotic plaques. This paper first explains 1-D radial strain estimation as applied intravascularly in coronary arteries. Next, recent methods for noninvasive vascular strain estimation in a transverse imaging plane are discussed. Finally, a compounding technique that our group recently developed is explained. This technique combines motion estimates of subsequently acquired focused ultrasound images obtained at various insonification angles. However, because the artery moves and deforms during the multi-angle acquisition, errors are introduced when compounding. Recent advances in computational power have enabled plane wave ultrasound acquisition, which allows 100 times faster image acquisition and thus might resolve the motion artifacts. In this paper the performance of strain imaging using plane wave compounding is investigated using simulations of an artery with a vulnerable plaque and experimental data of a two-layered vessel phantom. The results show that plane wave compounding outperforms 0° focused strain imaging. For the simulations, the root mean squared error reduced by 66% and 50% for radial and circumferential strain, respectively. For the experiments, the elastographic signal-to-noise and contrast-to-noise ratio (SNR(e) and CNR(e)) increased with 2.1 dB and 3.7 dB radially, and 5.6 dB and 16.2dB circumferentially. Because of the high frame rate, the plane wave compounding technique can even be further optimized and extended to 3D in future.

  4. Blue metal complex pigments involved in blue flower color

    PubMed Central

    Takeda, Kosaku

    2006-01-01

    The blue pigment of cornflower, protocyanin, has been investigated for a long time, but its precise structure was not entirely explained until recently. The molecular structure of the pigment was recently shown to be a metal complex of six molecules each of anthocyanin and flavone glycoside, with one ferric iron, one magnesium and two calcium ions by X-ray crystallographic analysis. The studies provided the answer to the question posed in the early part of the last century, “why is the cornflower blue and rose red when both flowers contain the same anthocyanin?” This work was achieved on the basis of the results of long years of the studies made by many researchers. In this review, the author focuses on the investigations of the blue metal complex pigments involved in the bluing of flowers, commelinin from Commelina commusis, protocyanin from Centaurea cyanus, protodelphin from Salvia patens and hydrangea blue pigment. PMID:25792777

  5. Blue metal complex pigments involved in blue flower color.

    PubMed

    Takeda, Kosaku

    2006-05-01

    The blue pigment of cornflower, protocyanin, has been investigated for a long time, but its precise structure was not entirely explained until recently. The molecular structure of the pigment was recently shown to be a metal complex of six molecules each of anthocyanin and flavone glycoside, with one ferric iron, one magnesium and two calcium ions by X-ray crystallographic analysis. The studies provided the answer to the question posed in the early part of the last century, "why is the cornflower blue and rose red when both flowers contain the same anthocyanin?" This work was achieved on the basis of the results of long years of the studies made by many researchers. In this review, the author focuses on the investigations of the blue metal complex pigments involved in the bluing of flowers, commelinin from Commelina commusis, protocyanin from Centaurea cyanus, protodelphin from Salvia patens and hydrangea blue pigment.

  6. The Laplace Planes of Uranus and Pluto

    NASA Technical Reports Server (NTRS)

    Dobrovolskis, Anthony R.

    1993-01-01

    Satellite orbits close to an oblate planet precess about its equatorial plane, while distant satellites precess around the plane of the planet's heliocentric orbit. In between, satellites in nearly circular orbits precess about a warped intermediate surface called the Laplace 'plane.' Herein we derive general formulas for locating the Laplace plane. Because Uranus and Pluto have high obliquities, their Laplace planes are severely warped. We present maps of these Laplace planes, of interest in telescopic searches for new satellites. The Laplace plane of the Solar System as a whole is similarly distorted, but comets in the inner Oort cloud precess too slowly to sense the Laplace plane.

  7. Focal-plane electric field sensing with pupil-plane holograms

    NASA Astrophysics Data System (ADS)

    Por, Emiel H.; Keller, Christoph U.

    2016-07-01

    The direct detection and spectral characterization of exoplanets requires a coronagraph to suppress the diffracted star light. Amplitude and phase aberrations in the optical train fill the dark zone of the coronagraph with quasi-static speckles that limit the achievable contrast. Focal-plane electric field sensing, such as phase diversity introduced by a deformable mirror (DM), is a powerful tool to minimize this residual star light. The residual electric field can be estimated by sequentially applying phase probes on the DM to inject star light with a well-known amplitude and phase into the dark zone and analyzing the resulting intensity images. The DM can then be used to add light with the same amplitude but opposite phase to destructively interfere with this residual star light. Using a static phase-only pupil-plane element we create holographic copies of the point spread function (PSF), each superimposed with a certain pupil-plane phase probe. We therefore obtain all intensity images simultaneously while still retaining a central, unaltered science PSF. The electric field sensing method only makes use of the holographic copies, allowing for correction of the residual electric field while retaining the central PSF for uninterrupted science data collection. In this paper we demonstrate the feasibility of this method with numerical simulations.

  8. Explosive plane-wave lens

    DOEpatents

    Marsh, S.P.

    1988-03-08

    An explosive plane-wave air lens which enables a spherical wave form to be converted to a planar wave without the need to specially machine or shape explosive materials is described. A disc-shaped impactor having a greater thickness at its center than around its periphery is used to convert the spherical wave into a plane wave. When the wave reaches the impactor, the center of the impactor moves first because the spherical wave reaches the center of the impactor first. The wave strikes the impactor later in time as one moves radially along the impactor. Because the impactor is thinner as one moves radially outward, the velocity of the impactor is greater at the periphery than at the center. An acceptor explosive is positioned so that the impactor strikes the acceptor simultaneously. Consequently, a plane detonation wave is propagated through the acceptor explosive. 4 figs.

  9. Explosive plane-wave lens

    DOEpatents

    Marsh, Stanley P.

    1988-01-01

    An explosive plane-wave air lens which enables a spherical wave form to be converted to a planar wave without the need to specially machine or shape explosive materials is described. A disc-shaped impactor having a greater thickness at its center than around its periphery is used to convert the spherical wave into a plane wave. When the wave reaches the impactor, the center of the impactor moves first because the spherical wave reaches the center of the impactor first. The wave strikes the impactor later in time as one moves radially along the impactor. Because the impactor is thinner as one moves radially outward, the velocity of the impactor is greater at the periphery than at the center. An acceptor explosive is positioned so that the impactor strikes the acceptor simultaneously. Consequently, a plane detonation wave is propagated through the acceptor explosive.

  10. Explosive plane-wave lens

    DOEpatents

    Marsh, S.P.

    1987-03-12

    An explosive plane-wave air lens which enables a spherical wave form to be converted to a planar wave without the need to specially machine or shape explosive materials is described. A disc-shaped impactor having a greater thickness at its center than around its periphery is used to convert the spherical wave into a plane wave. When the wave reaches the impactor, the center of the impactor moves first because the spherical wave reaches the center of the impactor first. The wave strikes the impactor later in time as one moves radially along the impactor. Because the impactor is thinner as one moves radially outward, the velocity of the impactor is greater at the periphery than at the center. An acceptor explosive is positioned so that the impactor strikes the acceptor simultaneously. Consequently, a plane detonation wave is propagated through the acceptor explosive. 3 figs., 3 tabs.

  11. Three Dimensional Sheaf of Ultrasound Planes Reconstruction (SOUPR) of Ablated Volumes

    PubMed Central

    Ingle, Atul; Varghese, Tomy

    2014-01-01

    This paper presents an algorithm for three dimensional reconstruction of tumor ablations using ultrasound shear wave imaging with electrode vibration elastography. Radiofrequency ultrasound data frames are acquired over imaging planes that form a subset of a sheaf of planes sharing a common axis of intersection. Shear wave velocity is estimated separately on each imaging plane using a piecewise linear function fitting technique with a fast optimization routine. An interpolation algorithm then computes velocity maps on a fine grid over a set of C-planes that are perpendicular to the axis of the sheaf. A full three dimensional rendering of the ablation can then be created from this stack of C-planes; hence the name “Sheaf Of Ultrasound Planes Reconstruction” or SOUPR. The algorithm is evaluated through numerical simulations and also using data acquired from a tissue mimicking phantom. Reconstruction quality is gauged using contrast and contrast-to-noise ratio measurements and changes in quality from using increasing number of planes in the sheaf are quantified. The highest contrast of 5 dB is seen between the stiffest and softest regions of the phantom. Under certain idealizing assumptions on the true shape of the ablation, good reconstruction quality while maintaining fast processing rate can be obtained with as few as 6 imaging planes suggesting that the method is suited for parsimonious data acquisitions with very few sparsely chosen imaging planes. PMID:24808405

  12. Simultaneous measurement of in-plane and out-of-plane displacement derivatives using dual-wavelength digital holographic interferometry.

    PubMed

    Rajshekhar, Gannavarpu; Gorthi, Sai Siva; Rastogi, Pramod

    2011-12-01

    The paper introduces a method for simultaneously measuring the in-plane and out-of-plane displacement derivatives of a deformed object in digital holographic interferometry. In the proposed method, lasers of different wavelengths are used to simultaneously illuminate the object along various directions such that a unique wavelength is used for a given direction. The holograms formed by multiple reference-object beam pairs of different wavelengths are recorded by a 3-color CCD camera with red, green, and blue channels. Each channel stores the hologram related to the corresponding wavelength and hence for the specific direction. The complex reconstructed interference field is obtained for each wavelength by numerical reconstruction and digital processing of the recorded holograms before and after deformation. Subsequently, the phase derivative is estimated for a given wavelength using two-dimensional pseudo Wigner-Ville distribution and the in-plane and out-of-plane components are obtained from the estimated phase derivatives using the sensitivity vectors of the optical configuration.

  13. Space-Plane Spreadsheet Program

    NASA Technical Reports Server (NTRS)

    Mackall, Dale

    1993-01-01

    Basic Hypersonic Data and Equations (HYPERDATA) spreadsheet computer program provides data gained from three analyses of performance of space plane. Equations used to perform analyses derived from Newton's second law of physics, derivation included. First analysis is parametric study of some basic factors affecting ability of space plane to reach orbit. Second includes calculation of thickness of spherical fuel tank. Third produces ratio between volume of fuel and total mass for each of various aircraft. HYPERDATA intended for use on Macintosh(R) series computers running Microsoft Excel 3.0.

  14. STREPTOMYCES SPECIES COMPRISING THE BLUE-SPORE SERIES

    PubMed Central

    Trejo, W. H.; Bennett, R. E.

    1963-01-01

    Trejo, W. H. (Squibb Institute for Medical Research, New Brunswick, N.J.) and R. E. Bennett. Streptomyces species comprising the blue-spore series. J. Bacteriol. 85:676–690. 1963.—The objective of this study was to define and delimit the streptomycetes of the blue-spored (Viridochromogenes) series. The series, as defined in this study, includes 11 blue and blue-green species. The green-spored species were excluded on the basis of morphology as well as color. It was proposed that NRRL B-1511 be designated as the neotype strain of Streptomyces viridochromogenes (Krainsky) Waksman and Henrici, and that IMRU 3761 be designated as the neotype for Streptomyces cyaneus (Krassilnikov) Waksman. Evidence was presented to show that physiological criteria cannot be used to differentiate these organisms below the series level. The major characteristics of the Viridochromogenes series are blue to blue-green spores borne in spirals, and chromogenicity (melanin-positive). Reverse color and spore morphology provide a basis for separation below the series level. Images PMID:14042949

  15. Prototypical colors of skin, green plant, and blue sky

    NASA Astrophysics Data System (ADS)

    Zeng, Huanzhao; Luo, Ronnier

    2013-02-01

    Colors of skin, green plant, and blue sky of digital photographic images were studied for modeling and detection of these three important memory color regions. The color modeling of these three regions in CIELAB and CAM02-UCS was presented, and the properties of these three color groups were investigated.

  16. Numerical investigation of lensless zoomable holographic multiple projections to tilted planes

    NASA Astrophysics Data System (ADS)

    Shimobaba, Tomoyoshi; Makowski, Michal; Kakue, Takashi; Okada, Naohisa; Endo, Yutaka; Hirayama, Ryuji; Hiyama, Daisuke; Hasegawa, Satoki; Nagahama, Yuki; Ito, Tomoyoshi

    2014-12-01

    This paper numerically investigates the feasibility of lensless zoomable holographic multiple projections to tilted planes. We have already developed lensless zoomable holographic single projection using scaled diffraction, which calculates diffraction between parallel planes with different sampling pitches. The structure of this zoomable holographic projection is very simple because it does not need a lens; however, it only projects a single image to a plane parallel to the hologram. The lensless zoomable holographic projection in this paper is capable of projecting multiple images onto tilted planes simultaneously.

  17. Affine Contractions on the Plane

    ERIC Educational Resources Information Center

    Celik, D.; Ozdemir, Y.; Ureyen, M.

    2007-01-01

    Contractions play a considerable role in the theory of fractals. However, it is not easy to find contractions which are not similitudes. In this study, it is shown by counter examples that an affine transformation of the plane carrying a given triangle onto another triangle may not be a contraction even if it contracts edges, heights or medians.…

  18. Photosensitizer methylene blue-semiconductor nanocrystals hybrid system for photodynamic therapy.

    PubMed

    Rakovich, Aliaksandra; Rakovich, Tatsiana; Kelly, Vincent; Lesnyak, Vladimir; Eychmüller, Alexander; Rakovich, Yury P; Donegan, John F

    2010-04-01

    In this work we report on the development of novel hybrid material with enhanced photodynamic properties based on methylene blue and CdTe nanocrystals. Absorption spectroscopy, visible photoluminescence spectroscopy and fluorescence lifetime imaging of this system reveal efficient charge transfer between nanocrystals and the methylene blue dye. Near infra-red photoluminescence measurements provide evidence for an increased efficiency of singlet oxygen production by the methylene blue dye. In vitro studies on the growth of HepG2 and HeLa cancerous cells were also performed, they point towards an improvement in the cell kill efficiency for the methylene blue-semiconductor nanocrystals hybrid system.

  19. Specimen housing unit for cinemicrographic studies in the vertical plane.

    PubMed Central

    Wilkins, J R; Tynan, C I; Boykin, E H

    1976-01-01

    A compact housing unit for low-power (X6 to X50) cinemicrographic studies of microbial specimens in the vertical plane is described. This unit was used successfully to record the development of a "halo" of cells around subsurface colonies of Escherichia coli. Images PMID:788640

  20. A perspective view of the plane mixing layer

    NASA Technical Reports Server (NTRS)

    Jimenez, J.; Cogollos, M.; Bernal, L. P.

    1984-01-01

    A three-dimensional model of the plane mixing layer is constructed by applying digital image processing and computer graphic techniques to laser fluorescent motion pictures of its transversal sections. A system of streamwise vortex pairs is shown to exist on top of the classical spanwise eddies. Its influence on mixing is examined.

  1. Integrating fringe projection and digital image correlation for high-quality measurements of shape changes

    NASA Astrophysics Data System (ADS)

    Felipe-Sesé, Luis; Siegmann, Philip; Díaz, Francisco A.; Patterson, Eann A.

    2014-04-01

    An approach for the measurement of surface displacement fields in three dimensions is presented based on the combination of two-dimensional digital image correlation with fringe projection. Only a single RGB image is required at each deformation state, thereby allowing real-time data acquisition, which is achieved using red speckle and projected blue fringes that are captured in the single image and separated using a Bayer filter. The approach allows both a perpendicular alignment relative to a flat reference surface and self-calibration, i.e., no calibration object is employed. The minimum measurement uncertainty of such a system is found to be 0.0083±0.00239 and 0.0238±0.0068 mm, respectively, for the in-plane and out-of-plane displacements. The potential of the approach is demonstrated for an elastic membrane undergoing large (5 to 20 mm) applied out-of-plane displacements, and the results show no significant difference (<1%) in the measured in-plane displacement fields compared with a commercially available system for stereoscopic digital image correlation.

  2. Numerical study for the calculation of computer-generated hologram in color holographic 3D projection enabled by modified wavefront recording plane method

    NASA Astrophysics Data System (ADS)

    Chang, Chenliang; Qi, Yijun; Wu, Jun; Yuan, Caojin; Nie, Shouping; Xia, Jun

    2017-03-01

    A method of calculating computer-generated hologram (CGH) for color holographic 3D projection is proposed. A color 3D object is decomposed into red, green and blue components. For each color component, a virtual wavefront recording plane (WRP) is established which is nonuniformly sampled according to the depth map of the 3D object. The hologram of each color component is calculated from the nonuniform sampled WRP using the shifted Fresnel diffraction algorithm. Finally three holograms of RGB components are encoded into one single CGH based on the multiplexing encoding method. The computational cost of CGH generation is reduced by converting diffraction calculation from huge 3D voxels to three 2D planar images. Numerical experimental results show that the CGH generated by our method is capable to project zoomable color 3D object with clear quality.

  3. Antimicrobial blue light therapy for Candida albicans burn infection in mice

    NASA Astrophysics Data System (ADS)

    Zhang, Yunsong; Wang, Yucheng; Murray, Clinton K.; Hamblin, Michael R.; Gu, Ying; Dai, Tianhong

    2015-05-01

    In this preclinical study, we investigated the utility of antimicrobial blue light therapy for Candida albicans infection in acutely burned mice. A bioluminescent strain of C. albicans was used. The susceptibilities to blue light inactivation were compared between C. albicans and human keratinocyte. In vitro serial passaging of C. albicans on blue light exposure was performed to evaluate the potential development of resistance to blue light inactivation. A mouse model of acute thermal burn injury infected with the bioluminescent strain of C. albicans was developed. Blue light (415 nm) was delivered to mouse burns for decolonization of C. albicans. Bioluminescence imaging was used to monitor in real time the extent of fungal infection in mouse burns. Experimental results showed that C. albicans was approximately 42-fold more susceptible to blue light inactivation in vitro than human keratinocyte (P=0.0022). Serial passaging of C. albicans on blue light exposure implied a tendency for the fungal susceptibility to blue light inactivation to decrease with the numbers of passages. Blue light reduced fungal burden by over 4-log10 (99.99%) in acute mouse burns infected with C. albicans in comparison to infected mouse burns without blue light therapy (P=0.015).

  4. Stokes problems for moving half-planes.

    PubMed

    Zeng, Y; Weinbaum, S

    1995-01-01

    New exact solutions of the Navier-Stokes equations are obtained for the unbounded and bounded oscillatory and impulsive tangential edgewise motion of touching half-infinite plates in their own plane. In contrast to Stokes classical solutions for the harmonic and impulsive motion of an infinite plane wall, where the solutions are separable or have a simple similarity form, the present solutions have a two-dimensional structure in the near region of the contact between the half-infinite plates. Nevertheless, it is possible to obtain relatively simple closed-form solutions for the flow field in each case by defining new variables which greatly simplify the r- and theta- dependence of the solutions in the vicinity of the contact region. These solutions for flow in a half-infinite space are then extended to bounded flows in a channel using an image superposition technique. The impulsive motion has application to the motion near geophysical faults, whereas the oscillatory motion has arisen in the design of a novel oscillating half-plate flow chamber for examining the effect of fluid shear stress on cultured cell monolayers.

  5. "Clothed in triple blues": sorting out the Italian blues.

    PubMed

    Bimler, David; Uusküla, Mari

    2014-04-01

    Cross-cultural comparisons of color perception and cognition often feature versions of the "similarity sorting" procedure. By interpreting the assignment of two color samples to different groups as an indication that the dissimilarity between them exceeds some threshold, sorting data can be regarded as low-resolution similarity judgments. Here we analyze sorting data from speakers of Italian, Russian, and English, applying multidimensional scaling to delineate the boundaries between perceptual categories while highlighting differences between the three populations. Stimuli were 55 color swatches, predominantly from the blue region. Results suggest that at least two Italian words for "blue" are basic, a similar situation to Russian, in contrast to English where a single "blue" term is basic.

  6. Eight plane IPND mechanical testing.

    SciTech Connect

    Zhao, A.; Guarino, V.; Wood, K.; Nephew, T.; Ayres, D.; Lee, A.; High Energy Physics; FNAL

    2008-03-18

    A mechanical test of an 8 plane IPND mechanical prototype, which was constructed using extrusions from the testing/tryout of the 16 cell prototype extrusion die in Argonne National Laboratory, was conducted. There were 4 vertical and 4 horizontal planes in this 8 plane IPND prototype. Each vertical plane had four 16 cell extrusions, while each horizontal plane had six 16 cell extrusions. Each plane was glued together using the formulation of Devcon adhesive, Devcon 60. The vertical extrusions used in the vertical planes shares the same dimensions as the horizontal extrusions in the horizontal planes with the average web thickness of 2.1 mm and the average wall thickness of 3.1 mm. This mechanical prototype was constructed with end-seals on the both ends of the vertical extrusions. The gaps were filled with epoxy between extrusions and end-seals. The overall dimension of IPND is 154.8 by 103.1 by 21.7 inches with the weight of approximately 1200 kg, as shown in a figure. Two similar mechanical tests of 3 layer and 11 layer prototypes have been done in order to evaluate the strength of the adhesive joint between extrusions in the NOvA detector. The test showed that the IPND prototype was able to sustain under the loading of weight of itself and scintillator. Two FEA models were built to verify the measurement data from the test. The prediction from FEA slice model seems correlated reasonably well to the test result, even under a 'rough' estimated condition for the wall thickness (from an untuned die) and an unknown property of 'garage type' extrusion. A full size of FEA 3-D model also agrees very well with the test data from strain gage readings. It is worthy to point out that the stress distribution of the structure is predominantly determined by the internal pressure, while the buckling stability relies more on the loading weight from the extrusions themselves and scintillate. Results of conducted internal pressure tests, including 3- cell, 11-cell and the IPND

  7. IUE observations of blue halo high luminosity stars

    NASA Technical Reports Server (NTRS)

    Hack, M.; Franco, M. L.; Stalio, R.

    1981-01-01

    Two high luminosity population II blue stars of high galactic latitude, BD+33 deg 2642 and HD 137569 were observed at high resolution. The stellar spectra show the effect of mass loss in BD+33 deg 2642 and abnormally weak metallic lines in HD 137569. The interstellar lines in the direction of BD+33 deg 2642, which lies at a height z greater than or equal to 6.2 kpc from the galactic plane, are split into two components. No high ionization stages are found at the low velocity component; nor can they be detected in the higher velocity clouds because of mixing with the corresponding stellar/circumstellar lines.

  8. Blue phase liquid crystals stabilized by linear photo-polymerization

    NASA Astrophysics Data System (ADS)

    Xu, Daming; Yuan, Jiamin; Schadt, Martin; Wu, Shin-Tson

    2014-08-01

    Stabilizing a photopolymer-embedded blue phase liquid crystal precursor with linearly polarized UV light is investigated experimentally. When the UV polarization axis is perpendicular to the stripe electrodes of an in-plane-switching cell, anisotropic polymer networks are formed through the linear photo-polymerization process and the electrostriction effect is suppressed. As a result, the measured hysteresis is dramatically reduced from 6.95% to 0.36% and the response time shortened by ˜2× compared to unpolarized UV exposure. To induce larger anisotropy in polymer networks for mitigating the electrostriction effect, high-intensity linearly polarized UV exposure is preferred.

  9. SETI in the Ecliptic Plane

    NASA Astrophysics Data System (ADS)

    Conn Henry, Richard; Kilston, S.; Shostak, S.

    2008-05-01

    The strong advantages of SETI searches in the ecliptic plane have been pointed out by Kilston, Shostak, and Henry (2008). In our poster we show one possible history of civilizations in the galaxy, from birth, through galactic colonization, up to death - and even beyond. Should this scenario be correct, the pattern suggests that the best hope for success in SETI is exploration of the possibility that there are a few extremely ancient but non-colonizing civilizations; civilizations that, aeons ago, detected the existence of Earth (oxygen, and hence life) and of its Moon (stabilizing Earth's rotation) via observations of transits of the Sun (hence, ecliptic, which is stable over millions of years [Laskar et al. 2004]), and have been beaming voluminous information in our direction ever since, in their faint hope (now realized) that a technological "receiving” species would appear. To maintain such a targeted broadcast would be extremely cheap for an advanced civilization. A search of a swath centered on our ecliptic plane should easily find such civilizations, if they exist. We hope to carry out such a search, using the Allen Telescope Array. http://henry.pha.jhu.edu/poster.SETI.pdf References: Kilston, Steven; Shostak, Seth; & Henry, Richard Conn; "Who's Looking at You, Kid?: SETI Advantages near the Ecliptic Plane," AbSciCon 2008, April 14-17, Santa Clara, CA.; Laskar, J., et al., A&A 428, 261, 2004 This work was supported by Maryland Space Grant Consortium.

  10. Optic flow and the metric of the visual ground plane.

    PubMed

    Beusmans, J M

    1998-04-01

    A theory is developed in which the optic flow of an observer translating over the ground plane determines the metric of egocentric visual space. Optic flow is used to operationalize the equality of spatial intervals not unlike physicists use time to compare spatial intervals. The theory predicts empirical matching ratios for collinear, sagittal intervals to within 2% of the mean (eight subjects, standard error also 2%). The theory predicts that frontoparallel intervals on the ground plane will match sagittal intervals if their relative image motions match, which was found empirically. It is suggested that the optic flow metric serves to calibrate static depth cues such as angular elevation and binocular parallax.

  11. THE WHIQII SURVEY: METALLICITIES AND SPECTROSCOPIC PROPERTIES OF LUMINOUS COMPACT BLUE GALAXIES

    SciTech Connect

    Tollerud, Erik J.; Barton, Elizabeth J.; Cooke, Jeff; Van Zee, Liese

    2010-01-10

    As part of the WIYN High Image Quality Indiana-Irvine (WHIQII) survey, we present 123 spectra of faint emission-line galaxies, selected to focus on intermediate redshift (0.4 approx< z approx< 0.8) galaxies with blue colors that appear physically compact on the sky. The sample includes 15 true Luminous Compact Blue Galaxies (LCBGs) and an additional 27 slightly less extreme emission-line systems. These galaxies represent a highly evolving class that may play an important role in the decline of star formation since z approx 1, but their exact nature and evolutionary pathways remain a mystery. Here, we use emission lines to determine metallicities and ionization parameters, constraining their intrinsic properties and state of star formation. Some LCBG metallicities are consistent with a 'bursting dwarf' scenario, while a substantial fraction of others are not, further confirming that LCBGs are a highly heterogeneous population but are broadly consistent with the intermediate redshift field. In agreement with previous studies, we observe overall evolution in the luminosity-metallicity relation at intermediate redshift. Our sample, and particularly the LCBGs, occupies a region in the empirical R{sub 23}-O{sub 32} plane that differs from luminous local galaxies and is more consistent with dwarf irregulars at the present epoch, suggesting that cosmic 'downsizing' is observable in even the most fundamental parameters that describe star formation. These properties for our sample are also generally consistent with lying between local galaxies and those at high redshift, as expected by this scenario. Surprisingly, our sample exhibits no detectable correlation between compactness and metallicity, strongly suggesting that at these epochs of rapid star formation, the morphology of compact star-forming galaxies is largely transient.

  12. Usage patterns of blue flower color representation by Encyclopedia of Life content providers

    PubMed Central

    Wright, Chantal-Marie

    2014-01-01

    Abstract Encyclopedia of Life (EOL) is a resource for community-driven biodiversity data, focusing on species information and images. Research into blue flowers to compare color ('blueness') at different elevations revealed that data content providers describe flowers as blue for any color hue in the range from blue to magenta. We propose methods for standardizing color values and color searching within EOL by means of an expanded color vocabulary and improved access to image metadata, in order to improve the research capacity of this valuable resource. PMID:25197234

  13. Usage patterns of blue flower color representation by Encyclopedia of Life content providers.

    PubMed

    Wright, Chantal-Marie; Seltmann, Katja C

    2014-01-01

    Encyclopedia of Life (EOL) is a resource for community-driven biodiversity data, focusing on species information and images. Research into blue flowers to compare color ('blueness') at different elevations revealed that data content providers describe flowers as blue for any color hue in the range from blue to magenta. We propose methods for standardizing color values and color searching within EOL by means of an expanded color vocabulary and improved access to image metadata, in order to improve the research capacity of this valuable resource.

  14. The BlueSky Smoke Modeling Framework: Recent Developments

    NASA Astrophysics Data System (ADS)

    Sullivan, D. C.; Larkin, N.; Raffuse, S. M.; Strand, T.; ONeill, S. M.; Leung, F. T.; Qu, J. J.; Hao, X.

    2012-12-01

    BlueSky systems—a set of decision support tools including SmartFire and the BlueSky Framework—aid public policy decision makers and scientific researchers in evaluating the air quality impacts of fires. Smoke and fire managers use BlueSky systems in decisions about prescribed burns and wildland firefighting. Air quality agencies use BlueSky systems to support decisions related to air quality regulations. We will discuss a range of recent improvements to the BlueSky systems, as well as examples of applications and future plans. BlueSky systems have the flexibility to accept basic fire information from virtually any source and can reconcile multiple information sources so that duplication of fire records is eliminated. BlueSky systems currently apply information from (1) the National Oceanic and Atmospheric Administration's (NOAA) Hazard Mapping System (HMS), which represents remotely sensed data from the Moderate Resolution Imaging Spectroradiometer (MODIS), Advanced Very High Resolution Radiometer (AVHRR), and Geostationary Operational Environmental Satellites (GOES); (2) the Monitoring Trends in Burn Severity (MTBS) interagency project, which derives fire perimeters from Landsat 30-meter burn scars; (3) the Geospatial Multi-Agency Coordination Group (GeoMAC), which produces helicopter-flown burn perimeters; and (4) ground-based fire reports, such as the ICS-209 reports managed by the National Wildfire Coordinating Group. Efforts are currently underway to streamline the use of additional ground-based systems, such as states' prescribed burn databases. BlueSky systems were recently modified to address known uncertainties in smoke modeling associated with (1) estimates of biomass consumption derived from sparse fuel moisture data, and (2) models of plume injection heights. Additional sources of remotely sensed data are being applied to address these issues as follows: - The National Aeronautics and Space Administration's (NASA) Tropical Rainfall Measuring Mission

  15. Validation of Multi-plane Particle Shadow Velocimetry to Quantify Turbulence Scales

    NASA Astrophysics Data System (ADS)

    Truong, Christine; Hinkle, Steven; Sinding, Kyle; Harris, Jeff; Krane, Michael; Jefferies, Rhett

    2016-11-01

    Estimates of radial integral length scales using multi-plane Particle Shadow Velocimetry (PSV) are presented using measurements from the 11.2-inch diameter glycerin tunnel in the Applied Research Lab Garfield Thomas Water Tunnel. Particle shadow velocimetry (PSV) enables illumination of a volume and is an efficient means of obtaining multi-plane illumination. The combination of two colors in the LED backlight and a dichroic mirror makes possible the imaging of two planes in space. Thus, velocity fields in two imaging planes separated radially along the optical axis can be simultaneously measured. These multi-plane velocity fields are correlated over a range of separations to obtain integral length scales. Integral time scales are also calculated and converted into a streamwise length scale using Taylor's hypothesis for further confirmation. The inter-plane radial length scales, the in-plane length scales, the converted time scale in the inter-plane radial direction, and multi-plane turbulent statistics are compared with published studies, which used proven measurement methods. An additional, independent check is provided from PSV measurements in a single radial-axial plane.

  16. Point-to-plane and plane-to-plane electrostatic charge injection atomization for insulating liquids

    NASA Astrophysics Data System (ADS)

    Malkawi, Ghazi

    An electrostatic charge injection atomizer was fabricated and used to introduce and study the electrostatic charge injection atomization methods for highly viscous vegetable oils and high conductivity low viscosity aviation fuel, JP8. The total, spray and leakage currents and spray breakup characteristics for these liquids were investigated and compared with Diesel fuel data. Jet breakup and spray atomization mechanism showed differences for vegetable oils and lower viscosity hydrocarbon fuels. For vegetable oils, a bending/spinning instability phenomenon was observed similar to the phenomenon found in liquid jets of high viscosity polymer solutions. The spray tip lengths and cone angles were presented qualitatively and quantitatively and correlated with the appropriate empirical formulas. The different stages of the breakup mechanisms for such oils, as a function of specific charges and flow rates, were discussed. In order to make this method of atomization more suitable for practical use in high flow rate applications, a blunt face electrode (plane-to-plane) was used as the charge emitter in place of a single pointed electrode (point-to-plane). This allowed the use of a multi-orifice emitter that maintained a specific charge with the flow rate increase which could not be achieved with the needle electrode. The effect of the nozzle geometry, liquid physical properties and applied bulk flow on the spray charge, total charge, maximum critical spray specific charge and electrical efficiency compared with the needle point-to-plane atomizer results was presented. Our investigation revealed that the electrical efficiency of the atomizer is dominated by the charge forced convection rate rather than charge transport by ion motilities and liquid motion by the electric field. As a result of the electric coulomb forces between the electrified jets, the multi-orifice atomizer provided a unique means of dispersing the fuel in a hollow cone with wide angles making the new

  17. Agminated blue nevus - Case report*

    PubMed Central

    Lisboa, Alice Paixão; Silvestre, Keline Jácome; Pedreira, Renata Leite; Alves, Natália Ribeiro de Magalhães; Obadia, Daniel Lago; Azulay-Abulafia, Luna

    2016-01-01

    Blue nevi are benign melanocytic lesions located in the deeper reticular dermis, consequence of failure of melanocytic migration into the dermal-epidermal junction from the neural crest. Lesions are usually asymptomatic and solitary, but may present in a multiple or agminated (grouped) pattern. The agminated subtype is formed when bluish-pigmented lesions cluster together in a well-defined area. Lesions can be flat or raised. We report the case of a patient who presented multiple bluish macules (1-3 mm in diameter) grouped on the left upper back. Dermoscopy and anatomic pathological examination were consistent with blue nevus. PMID:27828645

  18. Blue light emitting thiogallate phosphor

    DOEpatents

    Dye, Robert C.; Smith, David C.; King, Christopher N.; Tuenge, Richard T.

    1998-01-01

    A crystalline blue emitting thiogallate phosphor of the formula RGa.sub.2 S.sub.4 :Ce.sub.x where R is selected from the group consisting of calcium, strontium, barium and zinc, and x is from about 1 to 10 atomic percent, the phosphor characterized as having a crystalline microstructure on the size order of from about 100 .ANG. to about 10,000 .ANG. is provided together with a process of preparing a crystalline blue emitting thiogallate phosphor by depositing on a substrate by CVD and resultant thin film electroluminescent devices including a layer of such deposited phosphor on an ordinary glass substrate.

  19. Blue-green upconversion laser

    SciTech Connect

    Nguyen, Dinh C.; Faulkner, George E.

    1990-01-01

    A blue-green laser (450-550 nm) uses a host crystal doped with Tm.sup.3+. The Tm.sup.+ is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP.

  20. The Blue-Collar Brain

    PubMed Central

    Van Orden, Guy; Hollis, Geoff; Wallot, Sebastian

    2012-01-01

    Much effort has gone into elucidating control of the body by the brain, less so the role of the body in controlling the brain. This essay develops the idea that the brain does a great deal of work in the service of behavior that is controlled by the body, a blue-collar role compared to the white-collar control exercised by the body. The argument that supports a blue-collar role for the brain is also consistent with recent discoveries clarifying the white-collar role of synergies across the body’s tensegrity structure, and the evidence of critical phenomena in brain and behavior. PMID:22719730

  1. Blue-green upconversion laser

    DOEpatents

    Nguyen, D.C.; Faulkner, G.E.

    1990-08-14

    A blue-green laser (450--550 nm) uses a host crystal doped with Tm[sup 3+]. The Tm[sup 3+] is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP. 3 figs.

  2. Orbital Space Plane Cost Credibility

    NASA Technical Reports Server (NTRS)

    Creech, Steve

    2003-01-01

    NASA's largest new start development program is the Orbital Space Plane (OSP) Program. The program is currently in the formulation stage. One of the critical issues to be resolved, prior to initiating full-scale development, is establishing cost credibility of NASA s budget estimates for development, production, and operations of the OSP. This paper will discuss the processes, tools, and methodologies that NASA, along with its industry partners, are implementing to assure cost credibility for the OSP program. Results of benchmarking of current tools and the development of new cost estimating capabilities and approaches will be discussed.

  3. Augmented-plane-wave forces

    NASA Astrophysics Data System (ADS)

    Soler, José M.; Williams, Arthur R.

    1990-11-01

    Results are presented that demonstrate the effectiveness of a calculational method of electronic-structure theory. The method combines the power (tractable basis-set size) and flexibility (transition and first-row elements) of the augmented-plane-wave method with the computational efficiency of the Car-Parrinello method of molecular dynamics and total-energy minimization. Equilibrium geometry and vibrational frequencies in agreement with experiment are presented for Si, to demonstrate agreement with existing methods and for Cu, N2, and H2O to demonstrate the broader applicability of the approach.

  4. Blue Polar Dunes In False Color

    NASA Technical Reports Server (NTRS)

    2005-01-01

    [figure removed for brevity, see original site]

    The theme for the weeks of 1/17 and 1/24 is the north polar region of Mars as seen in false color THEMIS images. Ice/frost will typically appear as bright blue in color; dust mantled ice will appear in tones of red/orange.

    The small dunes in this image are 'bluer' than the rest of the layered ice/dust units to the left.

    Image information: VIS instrument. Latitude 84.5, Longitude 206.6 East (153.4 West). 19 meter/pixel resolution.

    Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time.

    NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in Pasadena.

  5. Blue Marble Western Hemisphere

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Drawing on data from multiple satellite missions (not all collected at the same time), a team of NASA scientists and graphic artists created layers of global data for everything from the land surface, to polar sea ice, to the light reflected by the chlorophyll in the billions of microscopic plants that grow in the ocean. They wrapped these layers around a globe, set it against a black background, and simulated the hazy edge of the Earth's atmosphere (the limb) that appears in astronaut photography of the Earth. The land surface layer is based on photo-like surface reflectance observations (reflected sunlight) measured by the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Terra satellite in July 2004. The sea ice layer near the poles comes from Terra MODIS observations of daytime sea ice observed between August 28 and September 6, 2001. The ocean layer is a composite. In shallow water areas, the layer shows surface reflectances observed by Terra MODIS in July 2004. In the open ocean, the photo-like layer is overlaid with observations of the average ocean chlorophyll content for 2004. NASA's Aqua MODIS collected the chlorophyll data. The cloud layer shows a single-day snapshot of clouds observed by Terra MODIS across the planet on July 29, 2001. City lights on Earth's night side are visualized from data collected by the Defense Meteorological Satellite Program mission between 1994-1995. The topography layer is based on radar data collected by the Space Shuttle Endeavour during an 11-day mission in February of 2000. Topography over Antarctica comes from the Radarsat Antarctic Mapping Project, version 2.

  6. Blue Marble Eastern Hemisphere

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Drawing on data from multiple satellite missions (not all collected at the same time), a team of NASA scientists and graphic artists created layers of global data for everything from the land surface, to polar sea ice, to the light reflected by the chlorophyll in the billions of microscopic plants that grow in the ocean. They wrapped these layers around a globe, set it against a black background, and simulated the hazy edge of the Earth's atmosphere (the limb) that appears in astronaut photography of the Earth. The land surface layer is based on photo-like surface reflectance observations (reflected sunlight) measured by the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Terra satellite in July 2004. The sea ice layer near the poles comes from Terra MODIS observations of daytime sea ice observed between August 28 and September 6, 2001. The ocean layer is a composite. In shallow water areas, the layer shows surface reflectances observed by Terra MODIS in July 2004. In the open ocean, the photo-like layer is overlaid with observations of the average ocean chlorophyll content for 2004. NASA's Aqua MODIS collected the chlorophyll data. The cloud layer shows a single-day snapshot of clouds observed by Terra MODIS across the planet on July 29, 2001. City lights on Earth's night side are visualized from data collected by the Defense Meteorological Satellite Program mission between 1994-1995. The topography layer is based on radar data collected by the Space Shuttle Endeavour during an 11-day mission in February of 2000. Topography over Antarctica comes from the Radarsat Antarctic Mapping Project, version 2.

  7. Application of high-resolution multispectral data for mapping blue ice areas in the Antarctic environment

    NASA Astrophysics Data System (ADS)

    Jawak, Shridhar D.; Luis, Alvarinho J.

    2016-05-01

    High resolution calibrated PAN-sharpened images from WorldView-2 (WV-2) were used for extracting blue ice areas in Schirmacher Oasis, east Antarctica. The Schirmacher oasis extends from 70°45' S to 70° 75' S and 11°38' E to 11° 38' E. Blue ice areas represents long-term ablation. The amplitude of blue ice is lower than that of snow, because the ice surface is smoother than the latter. But the difference is not so obvious when applying automatic extraction techniques. To achieve desirable results and support comparative analysis, multiband image combinations were generated from atmospherically-corrected WV-2 data. For feature extraction process, regions of interest (ROI) were considered in which blue ice was used as target and white snow/ice appearing on the blue ice was considered as non-target. Various semiautomatic feature extraction methods, such as, target detection, mapping methods, etc, and many trials were used for extracting blue ice areas. Surface patterns of alternating snow and blue ice bands were found in east Antarctica which becomes obstacle to clearly extract blue ice feature. From the high resolution WV-2 data, reference data (digitized data) were prepared for blue ice area. By comparing reference data and extracted data, bias and root mean square (RMS) error values were calculated. Accuracy assessment was done considering the entire necessary prior results of the blue ice area. Our results indicate that the pixel-based supervised classification methods yielded an overall accuracy ranging from 82%-89% for extraction of blue ice areas.

  8. The blue luminous events observed by ISUAL payload on board FORMOSAT-2 satellite

    NASA Astrophysics Data System (ADS)

    Kuo, C. L.; Su, H. T.; Hsu, R. R.

    2015-11-01

    We report the blue luminous events observed by the Imager of Sprite/Upper Atmospheric Lightning (ISUAL) payload on board the FORMOSAT-2 satellite. The ISUAL 427.8 nm filtered Imager campaign was conducted near Australia in the summer of the Southern Hemisphere during February-March 2008. On 18 February 2008, the 427.8 nm filtered Imager recorded a series of blue luminous events. One blue starter and nine smaller blue starters were recorded in 2 min and 34 s in a localized region with the radius < 4 km over the cloud top. The average time interval between subsequent blue luminous events was ~ 17 s. The occurrence rate of blue luminous events was 3.5 events per minute, slightly lower than the occurrence rate of pixies (4.2 events per minute) but higher than the occurrence rate of gnomes and blue jets in the previous observations. The recorded first blue starter lasted up to 2-3 frame times (60-90 ms) and extended its altitude about 8 ± 0.3 km with a width of ~2-4 km over the cloud top. After the first blue starter, subsequent nine smaller blue starters had the decreased heights of ~2-4 km, and their optical duration was shorter and is down to 1 ms. But their major emissions were 2PN2 and 1NN2+, without lightning OI 777.4 nm emission. The ISUAL recorded blue smaller starters had the spatial average brightness of 130 kR for the 427.8 nm filtered Imager with exposure time (29 ms) and 1.2 MR for the spectrophotometer (337 nm). Using the spectrophotometer, the emission time of blue starters was 1 ms. It is estimated that the 1NN2+ emission was ~ 22 MR and the 2PN2 emission was ~ 132 MR. We can estimate the degree of ionization was 10-11-10-12 in these blue luminous events using the 427.8 nm filtered Imager measured 1NN2+ (0,1) emission.

  9. Singing' the Black and Blues

    ERIC Educational Resources Information Center

    Fisher, Diane

    2004-01-01

    It is so obvious that the sky is blue in the daytime and black at night, but it took the smartest humans thousands of years of observation, thought, discussion, conjecture, and analysis to finally come up with answers that make scientific sense as to why the sky is these colors. This article discusses light and the scientific research…

  10. Teaching Sherman Alexie's "Reservation Blues."

    ERIC Educational Resources Information Center

    McFarland, Ronald E.

    2001-01-01

    A college teacher discusses his experiences of departing from the established literary canon to teach Sherman Alexie's "Reservation Blues" as part of an upper-level American literature survey class. Students reacted to the novel and its characters, evaluated Alexie's writing techniques, and discussed their personal experiences with Native…

  11. The Taos Blue Lake Ceremony.

    ERIC Educational Resources Information Center

    Bodine, John J.

    1988-01-01

    Describes the Blue Lake Ceremony of the Taos Pueblo Indians of New Mexico. Reproduces the 1906 account of the ceremony by anthropologist Matilda Coxe Stevenson and notes modern verification and change. Discusses the importance of this annual August pilgrimage and initiation rite to the preservation of Taos culture. (SV)

  12. Nobel Prize for blue LEDs

    NASA Astrophysics Data System (ADS)

    Kraftmakher, Yaakov

    2015-05-01

    A brief review of lighting technologies is presented. Unavoidable restrictions for incandescent light bulbs caused by the Planck distribution and properties of the human eye are illustrated. The efficiency and luminous efficacy of thermal radiation are calculated for various temperatures; the results clearly show the limitations for thermal radiators. The only way to overcome these limitations is using non-thermal radiators, such as fluorescent lamps and light-emitting diodes (LEDs). Unique advantages of LEDs undoubtedly made a revolution in this field. A crucial element of this progress is the blue LEDs (Nobel Prize 2014). Some experiments with a blue and a green LED are described: (i) the luminescence triggered in a green-yellow phosphor inside a white LED by the blue LED; (ii) radiant spectra and ‘efficiency droop’ in the LEDs; (iii) modulation of the blue LED up to 4 MHz; and (iv) the h/e ratio from the turn-on voltage of the green LED. The experiments are suitable for undergraduate laboratories and usable as classroom demonstrations.

  13. A compact bio-inspired visible/NIR imager for image-guided surgery (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Gao, Shengkui; Garcia, Missael; Edmiston, Chris; York, Timothy; Marinov, Radoslav; Mondal, Suman B.; Zhu, Nan; Sudlow, Gail P.; Akers, Walter J.; Margenthaler, Julie A.; Liang, Rongguang; Pepino, Marta; Achilefu, Samuel; Gruev, Viktor

    2016-03-01

    Inspired by the visual system of the morpho butterfly, we have designed, fabricated, tested and clinically translated an ultra-sensitive, light weight and compact imaging sensor capable of simultaneously capturing near infrared (NIR) and visible spectrum information. The visual system of the morpho butterfly combines photosensitive cells with spectral filters at the receptor level. The spectral filters are realized by alternating layers of high and low dielectric constant, such as air and cytoplasm. We have successfully mimicked this concept by integrating pixelated spectral filters, realized by alternating silicon dioxide and silicon nitrate layers, with an array of CCD detectors. There are four different types of pixelated spectral filters in the imaging plane: red, green, blue and NIR. The high optical density (OD) of all spectral filters (OD>4) allow for efficient rejections of photons from unwanted bands. The single imaging chip weighs 20 grams with form factor of 5mm by 5mm. The imaging camera is integrated with a goggle display system. A tumor targeted agent, LS301, is used to identify all spontaneous tumors in a transgenic PyMT murine model of breast cancer. The imaging system achieved sensitivity of 98% and selectivity of 95%. We also used our imaging sensor to locate sentinel lymph nodes (SLNs) in patients with breast cancer using indocyanine green tracer. The surgeon was able to identify 100% of SLNs when using our bio-inspired imaging system, compared to 93% when using information from the lymphotropic dye and 96% when using information from the radioactive tracer.

  14. Application of morphological bit planes in retinal blood vessel extraction.

    PubMed

    Fraz, M M; Basit, A; Barman, S A

    2013-04-01

    The appearance of the retinal blood vessels is an important diagnostic indicator of various clinical disorders of the eye and the body. Retinal blood vessels have been shown to provide evidence in terms of change in diameter, branching angles, or tortuosity, as a result of ophthalmic disease. This paper reports the development for an automated method for segmentation of blood vessels in retinal images. A unique combination of methods for retinal blood vessel skeleton detection and multidirectional morphological bit plane slicing is presented to extract the blood vessels from the color retinal images. The skeleton of main vessels is extracted by the application of directional differential operators and then evaluation of combination of derivative signs and average derivative values. Mathematical morphology has been materialized as a proficient technique for quantifying the retinal vasculature in ocular fundus images. A multidirectional top-hat operator with rotating structuring elements is used to emphasize the vessels in a particular direction, and information is extracted using bit plane slicing. An iterative region growing method is applied to integrate the main skeleton and the images resulting from bit plane slicing of vessel direction-dependent morphological filters. The approach is tested on two publicly available databases DRIVE and STARE. Average accuracy achieved by the proposed method is 0.9423 for both the databases with significant values of sensitivity and specificity also; the algorithm outperforms the second human observer in terms of precision of segmented vessel tree.

  15. HgCdTe hybrid focal plane

    NASA Astrophysics Data System (ADS)

    Rode, J. P.

    1984-09-01

    Second-generation IR systems, consisting of 2-D mosaics of IR detectors, have been under intense development for the last few years. One of the most successful architectures has been a HgCdTe hybrid focal plane array (FPA), using a Si charge-coupled device (CCD) readout chip interfaced to epitaxial HgCdTe. Detection is made by backside-illuminated photovoltaic detectors with high fill factors and quantum efficiency. The detectors are coupled into the CCD by In bumps which mass bond each detector in the mosaic to a CCD input. Advances have been made in uniform, large area HgCdTe detector material that can be grown with a bandgap from less than 0.1 eV to greater than 1 eV. CCD architectures have been developed with simple, linear inputs and dynamic ranges up to 80 dB. Hybrid FPAs are currently being tested in prototype imaging systems, for detecting thermal differences as well as reflected sunlight in the IR. In the 3-5μm region, these arrays have proven capable of noise-equivalent temperature differences as low as 0.01 K, acquired at a 400 Hz frame rate. In addition to improving current imaging systems, these area arrays allow new system concepts to be brought to fruition.

  16. Artificial impedance ground planes for low profile antenna applications

    NASA Astrophysics Data System (ADS)

    McMichael, Ian T.

    Recent interest in artificial impedance surfaces for low-profile antennas has led to extensive research with the goal of optimizing the ground plane's characteristics for a given antenna configuration and broadening the operational bandwidth, or alternatively creating a multi-band functionality. A method of determining the optimal reflection phase for a low-profile dipole antenna over an electromagnetic band gap (EBG) ground plane has been developed based on image theory and is presented with experimental and numerical validation. A new artificial impedance surface has also been developed, which is composed of an annular slot ring on a thin grounded dielectric. The main difference between the proposed ground plane and a conventional EBG is that the high impedance condition exists only in the vicinity of the slot and is therefore best suited for antennas with a current distribution that has a similar shape as the annular slot ring. It is shown that a loop antenna positioned closely over an annular slot loaded ground plane exhibits approximately the same gain as a loop antenna over a conventional EBG ground plane. The advantage of the new structure is its lack of periodicity, which significantly eases manufacturing. Additionally, it is shown that multiple concentric slot rings can be designed into the ground plane, which excites multiple resonances in low-profile wideband antennas. The result is a multi-band high impedance ground plane constructed using a simple arrangement of annular slots. Finally, a manufacturing technique is presented for the application of arbitrarily configured EBG antennas to handheld dual-sensor landmine detection systems. It is shown that creating an EBG antenna using very thin layers of metal will enable it to be used for ground penetrating radar (GPR) when it is co-located with a low frequency metal detector without compromising the operation of the metal detector. The potential benefit of such an antenna would be a lower profile sensor

  17. The Bolocam Galactic Plane Survey

    NASA Technical Reports Server (NTRS)

    Glenn, Jason; Aguirre, James; Bally, John; Battersby, Cara; Bradley, Eric Todd; Cyganowski, Claudia; Dowell, Darren; Drosback, Meredith; Dunham, Miranda K.; Evans, Neal J., II; Ginsburg, Adam; Harvey, Paul; Rosolowsky, Erik; Schlingman, Wayne; Shirley, Yancy L.; Stringfellow, Guy S.; Walawender, Josh; Williams, Jonathan

    2009-01-01

    The Bolocam Galactic Plane Survey (BGPS) is a 1.1 millimeter continuum survey of the northern Galactic Plane made with Bolocam and the Caltech Submillimeter Observatory. The coverage totals 170 square degrees, comprised of a contiguous range from -10.5 deg is less than or equal to 90.5 deg, 0.5 deg is less than or equal to b is less than or equal to 0.5 deg, with extended coverage in b in selected regions, and four targeted regions in the outer Galaxy, including: IC1396, toward the Perseus arm at l is approximately 111 deg, W3/4/5, and Gem OB1. Depths of the maps range from 30 to 60 mJy beam (sup 1). Approximately 8,400 sources were detected and the maps and source catalog have been made publicly available. Millimeter-wave thermal dust emission reveals dense regions within molecular clouds, thus the BGPS serves as a database for studies of the dense interstellar medium and star formation within the Milky Way.

  18. Singularities from colliding plane gravitational waves

    NASA Astrophysics Data System (ADS)

    Tipler, Frank J.

    1980-12-01

    A simple geometrical argument is given which shows that a collision between two plane gravitational waves must result in singularities. The argument suggests that these singularities are a peculiar feature of plane waves, because singularities are also a consequence of a collision between self-gravitating plane waves of other fields with arbitrarily small energy density.

  19. Blue Marble: Remote Characterization of Habitable Planets

    NASA Technical Reports Server (NTRS)

    Woolf, Neville; Lewis, Brian; Chartres, James; Genova, Anthony

    2009-01-01

    The study of the nature and distribution of habitable environments beyond the Solar System is a key area for Astrobiology research. At the present time, our Earth is the only habitable planet that can be characterized in the same way that we might characterize planets beyond the Solar System. Due to limitations in our current and near-future technology, it is likely that extra-solar planets will be observed as single-pixel objects. To understand this data, we must develop skills in analyzing and interpreting the radiation obtained from a single pixel. These skills must include the study of the time variation of the radiation, and the range of its photometric, spectroscopic and polarimetric properties. In addition, to understand whether we are properly analyzing the single pixel data, we need to compare it with a ground truth of modest resolution images in key spectral bands. This paper discusses the concept for a mission called Blue Marble that would obtain data of the Earth using a combination of spectropolarimetry, spectrophotometry, and selected band imaging. To obtain imagery of the proper resolution, it is desirable to place the Blue Marble spacecraft no closer than the outer region of cis-lunar space. This paper explores a conceptual mission design that takes advantage of low-cost launchers, bus designs and mission elements to provide a cost effective observing platform located at one of the stable Earth-moon Lagrangian points (L4, L5). The mission design allows for the development and use of novel technologies, such as a spinning moon sensor for attitude control, and leverages lessons-learned from previous low-cost spacecraft such as Lunar Prospector to yield a low-risk mission concept.

  20. Quantum-Well Infrared Photodetector (QWIP) Focal Plane Assembly

    NASA Technical Reports Server (NTRS)

    Jhabvala, Murzy; Jhabvala, Christine A.; Ewin, Audrey J.; Hess, Larry A.; Hartmann, Thomas M.; La, Anh T.

    2012-01-01

    A paper describes the Thermal Infrared Sensor (TIRS), a QWIP-based instrument intended to supplement the Operational Land Imager (OLI) for the Landsat Data Continuity Mission (LDCM). The TIRS instrument is a far-infrared imager operating in the pushbroom mode with two IR channels: 10.8 and 12 microns. The focal plane will contain three 640x512 QWIP arrays mounted on a silicon substrate. The silicon substrate is a custom-fabricated carrier board with a single layer of aluminum interconnects. The general fabrication process starts with a 4-in. (approx.10-cm) diameter silicon wafer. The wafer is oxidized, a single substrate contact is etched, and aluminum is deposited, patterned, and alloyed. This technology development is aimed at incorporating three large-format infrared detecting arrays based on GaAs QWIP technology onto a common focal plane with precision alignment of all three arrays. This focal plane must survive the rigors of flight qualification and operate at a temperature of 43 K (-230 C) for five years while orbiting the Earth. The challenges presented include ensuring thermal compatibility among all the components, designing and building a compact, somewhat modular system and ensuring alignment to very tight levels. The multi-array focal plane integrated onto a single silicon substrate is a new application of both QWIP array development and silicon wafer scale integration. The Invar-based assembly has been tested to ensure thermal reliability.

  1. 21 CFR 73.50 - Ultramarine blue.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... ultramarine blue is a blue pigment obtained by calcining a mixture of kaolin, sulfur, sodium carbonate, and... order to vary the shade. The pigment is a complex sodium aluminum sulfo-silicate having the...

  2. 21 CFR 73.50 - Ultramarine blue.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... ultramarine blue is a blue pigment obtained by calcining a mixture of kaolin, sulfur, sodium carbonate, and... order to vary the shade. The pigment is a complex sodium aluminum sulfo-silicate having the...

  3. 21 CFR 73.50 - Ultramarine blue.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... ultramarine blue is a blue pigment obtained by calcining a mixture of kaolin, sulfur, sodium carbonate, and... order to vary the shade. The pigment is a complex sodium aluminum sulfo-silicate having the...

  4. 21 CFR 73.50 - Ultramarine blue.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ultramarine blue is a blue pigment obtained by calcining a mixture of kaolin, sulfur, sodium carbonate, and... order to vary the shade. The pigment is a complex sodium aluminum sulfo-silicate having the...

  5. 21 CFR 73.50 - Ultramarine blue.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... ultramarine blue is a blue pigment obtained by calcining a mixture of kaolin, sulfur, sodium carbonate, and... order to vary the shade. The pigment is a complex sodium aluminum sulfo-silicate having the...

  6. Eta Carinae: Orientation of The Orbital Plane

    NASA Technical Reports Server (NTRS)

    Gull, T. R.; Nielsen, K. E.; Ivarsson, S.; Corcoran, M. F.; Verner, E.; Hillier, J. D.

    2006-01-01

    Evidence continues to build that Eta Carinae is a massive binary system with a hidden hot companion in a highly elliptical orbit. We present imaging and spectroscopic evidence that provide clues to the orientation of the orbital plane. The circumstellar ejecta, known as the Homunculus and Little Homunculus, are hourglass-shaped structures, one encapsulated within the other, tilted at about 45 degrees from the sky plane. A disk region lies between the bipolar lobes. Based upon their velocities and proper motions, Weigelt blobs B, C and D, very bright emission clumps 0.1 to 0.3" Northwest from Eta Carinae, lie in the disk. UV flux from the hot companion, Eta Car B, photoexcites the Weigelt blobs. Other clumps form a complete chain around the star, but are not significantly photoexcited. The strontium filament, a 'neutral' emission structure, lies in the same general direction as the Weigelt blobs and exhibits peculiar properties indicative that much mid-UV, but no hydrogen-ionizing radiation impinges on this structure. It is shielded by singly-ionized iron. P Cygni absorptions in Fe I I lines, seen directly in line of sight from Eta Carinae, are absent in the stellar light scattered by the Weigelt blobs. Rather than a strong absorption extending to -600 km/s, a low velocity absorption feature extends from -40 to -150 km/s. No absorbing Fe II exists between Eta Carinae and Weigelt D, but the outer reaches of the wind are intercepted in line of sight from Weigelt D to the observer. This indicates that the UV radiation is constrained by the dominating wind of Eta Car A to a small cavity carved out by the weaker wind of Eta Car B. Since the high excitation nebular lines are seen in the Weigelt blobs at most phases, the cavity, and hence the major axis of the highly elliptical orbit, must lie in the general direction of the Weigelt blobs. The evidence is compelling that the orbital major axis of Eta Carinae is projected at -45 degrees position angle on the sky. Moreover

  7. Geometric plane shapes for computer-generated holographic engraving codes

    NASA Astrophysics Data System (ADS)

    Augier, Ángel G.; Rabal, Héctor; Sánchez, Raúl B.

    2017-04-01

    We report a new theoretical and experimental study on hologravures, as holographic computer-generated laser-engravings. A geometric theory of images based on the general principles of light ray behaviour is shown. The models used are also applicable for similar engravings obtained by any non-laser method, and the solutions allow for the analysis of particular situations, not only in the case of light reflection mode, but also in transmission mode geometry. This approach is a novel perspective allowing the three-dimensional (3D) design of engraved images for specific ends. We prove theoretically that plane curves of very general geometric shapes can be used to encode image information onto a two-dimensional (2D) engraving, showing notable influence on the behaviour of reconstructed images that appears as an exciting investigation topic, extending its applications. Several cases of code using particular curvilinear shapes are experimentally studied. The computer-generated objects are coded by using the chosen curve type, and engraved by a laser on a plane surface of suitable material. All images are recovered optically by adequate illumination. The pseudoscopic or orthoscopic character of these images is considered, and an appropriate interpretation is presented.

  8. Signal processing on the focal plane array: an overview

    NASA Astrophysics Data System (ADS)

    Graham, Roger W.; Trautfield, Walter C.; Taylor, Scott M.; Murray, Mark P.; Mesh, Frank J.; Horn, Stuart B.; Finch, James A.; Dang, Khoa V.; Caulfield, John T.

    2000-12-01

    Raytheon's Infrared Operations (RIO) has invented and developed a new class of focal plane arrays; the Adaptive IR Sensor (AIRS) and Thinfilm Analog Image Processor (TAIP). The AIRS FPA is based upon biologically inspired on-focal- plane circuitry, which adaptively removes detector and optic temperature drift and l/f induced fixed pattern noise. This third-generation multimode IRFPA, also called a Smart FPA, is a 256x256-array format capable of operation in four modes: 1) Direct Injection (DI), 2) Adaptive Non-uniformity Correction (NUC), 3) Motion/Edge Detection, and 4) Subframe Averaging. Also the 320x240 TAIP results have shown excellent image processing in the form of Spatial and Temporal processing.

  9. Functional Aesthetic Occlusal Plane (FAOP)

    PubMed Central

    Câmara, Carlos Alexandre; Martins, Renato Parsekian

    2016-01-01

    ABSTRACT Introduction: A reasonable exposure of incisors and gingival tissues is generally considered more attractive than excess or lack of exposure. A reasonable gingival exposure is considered to be around 0 to 2 mm when smiling and 2-4 mm exposure of the maxillary incisor edge when the lips are at rest. Objective: The aim of this paper is to present the Functional Aesthetic Occlusal Plane (FAOP), which aims to help in the diagnosis of the relationships established among molars, incisors and the upper lip. Conclusion: FAOP can complement an existing and established orthodontic treatment plan, facilitating the visualization of functional and aesthetic demands by giving a greater focus on the position of incisors in the relationship established among the incisors, molars and the upper lip stomion. PMID:27653271

  10. Thermodynamics of black plane solution

    NASA Astrophysics Data System (ADS)

    Rodrigues, Manuel E.; Jardim, Deborah F.; Houndjo, Stéphane J. M.; Myrzakulov, Ratbay

    2013-11-01

    We obtain a new phantom black plane solution in D of the Einstein-Maxwell theory coupled with a cosmological constant. We analyse their basic properties, as well as its causal structure, and obtain the extensive and intensive thermodynamic variables, as well as the specific heat and the first law. Through the specific heat and the so-called geometric methods, we analyse in detail their thermodynamic properties, the extreme and phase transition limits, as well as the local and global stabilities of the system. The normal case is shown with an extreme limit and the phantom one with a phase transition only for null mass, which is physically inaccessible. The systems present local and global stabilities for certain values of the entropy density with respect to the electric charge, for the canonical and grand canonical ensembles.

  11. Smov Baseline Focal Plane Check

    NASA Astrophysics Data System (ADS)

    Gilmozzi, Roberto

    1994-01-01

    This test will be executed during the period after the servicing mission and before the extension of the COSTAR assembly. Its purpose is to verify that the FOS, HRS, and FOC focal planes have not been altered by the activities performed by Story and the Astronauts during the servicing mission. A large unknown deviation in aperture position would severly impact subsequent COSTAR alignment activities. If this test reveals a deviation, we may be able to compensate for any offsets prior to the complex and delicate COSTAR alignment calibrations. This enhanced version of the Heptathlon is designed to verify course alignments and measure relative aperture positions to within a few arcsecs. SPECIAL REQUIREMENTS: ***This test uses pre-servicing parameters for HRS, FOS, and FOC and the Cycle 4 parameters for WFPC2.*** ***This test requires special alignment and special guide stars.** ***This test requires special commanding for telemetry setups.**

  12. Snakes Out of the Plane

    NASA Astrophysics Data System (ADS)

    McCormick, Andrew; Young, Bruce A.; Mahadevan, L.

    2012-02-01

    We develop a new computational model of elastic rods, taking into account shear and full rotational dynamics, as well as friction, adhesion, and collision. This model is used to study the movement of snakes in different environments. By applying different muscular activation patterns to the snake, we observe many different patterns of motion, from planar undulation to sudden strikes. Many of the most interesting behaviors involve the snake rising out of the horizontal plane in the vertical direction. Such behaviors include a sand snake sidewinding over the hot desert sand and a cobra rearing up into a defensive striking position. Experimental videos of live snakes are analyzed and compared with computational results. We identify and explain a new form of movement previously unobserved: ``collateral locomotion.''

  13. Broken chiral symmetry on a null plane

    SciTech Connect

    Beane, Silas R.

    2013-10-15

    On a null-plane (light-front), all effects of spontaneous chiral symmetry breaking are contained in the three Hamiltonians (dynamical Poincaré generators), while the vacuum state is a chiral invariant. This property is used to give a general proof of Goldstone’s theorem on a null-plane. Focusing on null-plane QCD with N degenerate flavors of light quarks, the chiral-symmetry breaking Hamiltonians are obtained, and the role of vacuum condensates is clarified. In particular, the null-plane Gell-Mann–Oakes–Renner formula is derived, and a general prescription is given for mapping all chiral-symmetry breaking QCD condensates to chiral-symmetry conserving null-plane QCD condensates. The utility of the null-plane description lies in the operator algebra that mixes the null-plane Hamiltonians and the chiral symmetry charges. It is demonstrated that in a certain non-trivial limit, the null-plane operator algebra reduces to the symmetry group SU(2N) of the constituent quark model. -- Highlights: •A proof (the first) of Goldstone’s theorem on a null-plane is given. •The puzzle of chiral-symmetry breaking condensates on a null-plane is solved. •The emergence of spin-flavor symmetries in null-plane QCD is demonstrated.

  14. Argand-plane vorticity singularities in complex scalar optical fields: an experimental study using optical speckle.

    PubMed

    Rothschild, Freda; Bishop, Alexis I; Kitchen, Marcus J; Paganin, David M

    2014-03-24

    The Cornu spiral is, in essence, the image resulting from an Argand-plane map associated with monochromatic complex scalar plane waves diffracting from an infinite edge. Argand-plane maps can be useful in the analysis of more general optical fields. We experimentally study particular features of Argand-plane mappings known as "vorticity singularities" that are associated with mapping continuous single-valued complex scalar speckle fields to the Argand plane. Vorticity singularities possess a hierarchy of Argand-plane catastrophes including the fold, cusp and elliptic umbilic. We also confirm their connection to vortices in two-dimensional complex scalar waves. The study of vorticity singularities may also have implications for higher-dimensional fields such as coherence functions and multi-component fields such as vector and spinor fields.

  15. The Physics of the Blues

    NASA Astrophysics Data System (ADS)

    Gibson, J. Murray

    2009-03-01

    In looking at the commonalities between music and science, one sees that the musician's palette is based on the principles of physics. The pitch of a musical note is determined by the frequency of the sound wave. The scales that musicians use to create and play music can be viewed as a set of rules. What makes music interesting is how musicians develop those rules and create ambiguity with them. I will discuss the evolution of western musical scales in this context. As a particular example, ``Blue'' notes are very harmonic notes that are missing from the equal temperament scale. The techniques of piano blues and jazz represent the melding of African and Western music into something totally new and exciting. Live keyboard demonstrations will be used. Beyond any redeeming entertainment value the talk will emphasize the serious connections between science and art in music. Nevertheless tips will be accepted.

  16. Blue phases of cholesteryl nonanoate

    NASA Astrophysics Data System (ADS)

    Meiboom, S.; Sammon, M.

    1981-07-01

    The transformation on heating of an ordinary (helical) cholesteric liquid crystal (CHOL) into the isotropic phase (ISO) often occurs via a number of intermediate "blue" phases. We find the following scheme of phase transitions in cholesteryl nonanoate: CHOL-->91.35BPI-->91.76BPII-->91.84BPIII-->91.95ISO. Here BPI, BPII, and BPIII indicate three distinct, thermodynamically stable phases; transition temperatures are in °C. From observations of supercooling and coexistence, we conclude that all these transformations are first order, except possibly the BPIII-->ISO, the character of which remains in doubt. A similar behavior is found in cholesteryl myristate and in a mixture of cholesteryl nonanoate and cholesteryl chloride. A few observations having a bearing on the structure of the blue phases are reported.

  17. Status of Blue Ridge Reservoir

    SciTech Connect

    Not Available

    1990-09-01

    This is one in a series of reports prepared by the Tennessee Valley Authority (TVA) for those interested in the conditions of TVA reservoirs. This overview of Blue Ridge Reservoir summarizes reservoir and watershed characteristics, reservoir uses and use impairments, water quality and aquatic biological conditions, and activities of reservoir management agencies. This information was extracted from the most current reports and data available, as well as interview with water resource professionals in various federal, state, and local agencies. Blue Ridge Reservoir is a single-purpose hydropower generating project. When consistent with this primary objective, the reservoir is also operated to benefit secondary objectives including water quality, recreation, fish and aquatic habitat, development of shoreline, aesthetic quality, and other public and private uses that support overall regional economic growth and development. 8 refs., 1 fig.

  18. Ol' Blue Eyes, in Focus

    ERIC Educational Resources Information Center

    Nelson, Michael

    2009-01-01

    Scholarly books with "identity" and "culture" in the title have loomed large on academic publishing lists for several years. Scholarly books with "Sinatra" in the title are a more recent phenomenon. Despite his six-decade career as the Voice (the 1940s), the Chairman of the Board (the 50s and 60s), and Ol' Blue Eyes (the 70s through his death, in…

  19. Models of Individual Blue Stragglers

    NASA Astrophysics Data System (ADS)

    Sills, Alison

    This chapter describes the current state of models of individual blue stragglers. Stellar collisions, binary mergers (or coalescence), and partial or ongoing mass transfer have all been studied in some detail. The products of stellar collisions retain memory of their parent stars and are not fully mixed. Very high initial rotation rates must be reduced by an unknown process to allow the stars to collapse to the main sequence. The more massive collision products have shorter lifetimes than normal stars of the same mass, while products between low mass stars are long-lived and look very much like normal stars of their mass. Mass transfer can result in a merger, or can produce another binary system with a blue straggler and the remnant of the original primary. The products of binary mass transfer cover a larger portion of the colour-magnitude diagram than collision products for two reasons: there are more possible configurations which produce blue stragglers, and there are differing contributions to the blended light of the system. The effects of rotation may be substantial in both collision and merger products, and could result in significant mixing unless angular momentum is lost shortly after the formation event. Surface abundances may provide ways to distinguish between the formation mechanisms, but care must be taken to model the various mixing mechanisms properly before drawing strong conclusions. Avenues for future work are outlined.

  20. The Cryptochrome Blue Light Receptors

    PubMed Central

    Yu, Xuhong; Liu, Hongtao; Klejnot, John; Lin, Chentao

    2010-01-01

    Cryptochromes are photolyase-like blue light receptors originally discovered in Arabidopsis but later found in other plants, microbes, and animals. Arabidopsis has two cryptochromes, CRY1 and CRY2, which mediate primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, respectively. In addition, cryptochromes also regulate over a dozen other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. Cryptochromes have two domains, the N-terminal PHR (Photolyase-Homologous Region) domain that bind the chromophore FAD (flavin adenine dinucleotide), and the CCE (CRY C-terminal Extension) domain that appears intrinsically unstructured but critical to the function and regulation of cryptochromes. Most cryptochromes accumulate in the nucleus, and they undergo blue light-dependent phosphorylation or ubiquitination. It is hypothesized that photons excite electrons of the flavin molecule, resulting in redox reaction or circular electron shuttle and conformational changes of the photoreceptors. The photoexcited cryptochrome are phosphorylated to adopt an open conformation, which interacts with signaling partner proteins to alter gene expression at both transcriptional and posttranslational levels and consequently the metabolic and developmental programs of plants. PMID:21841916

  1. The Cryptochrome Blue Light Receptors.

    PubMed

    Yu, Xuhong; Liu, Hongtao; Klejnot, John; Lin, Chentao

    2010-09-23

    Cryptochromes are photolyase-like blue light receptors originally discovered in Arabidopsis but later found in other plants, microbes, and animals. Arabidopsis has two cryptochromes, CRY1 and CRY2, which mediate primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, respectively. In addition, cryptochromes also regulate over a dozen other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. Cryptochromes have two domains, the N-terminal PHR (Photolyase-Homologous Region) domain that bind the chromophore FAD (flavin adenine dinucleotide), and the CCE (CRY C-terminal Extension) domain that appears intrinsically unstructured but critical to the function and regulation of cryptochromes. Most cryptochromes accumulate in the nucleus, and they undergo blue light-dependent phosphorylation or ubiquitination. It is hypothesized that photons excite electrons of the flavin molecule, resulting in redox reaction or circular electron shuttle and conformational changes of the photoreceptors. The photoexcited cryptochrome are phosphorylated to adopt an open conformation, which interacts with signaling partner proteins to alter gene expression at both transcriptional and posttranslational levels and consequently the metabolic and developmental programs of plants.

  2. Improved wheal detection from skin prick test images

    NASA Astrophysics Data System (ADS)

    Bulan, Orhan

    2014-03-01

    Skin prick test is a commonly used method for diagnosis of allergic diseases (e.g., pollen allergy, food allergy, etc.) in allergy clinics. The results of this test are erythema and wheal provoked on the skin where the test is applied. The sensitivity of the patient against a specific allergen is determined by the physical size of the wheal, which can be estimated from images captured by digital cameras. Accurate wheal detection from these images is an important step for precise estimation of wheal size. In this paper, we propose a method for improved wheal detection on prick test images captured by digital cameras. Our method operates by first localizing the test region by detecting calibration marks drawn on the skin. The luminance variation across the localized region is eliminated by applying a color transformation from RGB to YCbCr and discarding the luminance channel. We enhance the contrast of the captured images for the purpose of wheal detection by performing principal component analysis on the blue-difference (Cb) and red-difference (Cr) color channels. We finally, perform morphological operations on the contrast enhanced image to detect the wheal on the image plane. Our experiments performed on images acquired from 36 different patients show the efficiency of the proposed method for wheal detection from skin prick test images captured in an uncontrolled environment.

  3. Focal plane detectors possible detector technologies for OWL/AIRWATCH

    SciTech Connect

    Flyckt, Esso

    1998-06-15

    New satellite-born projects OWL and AIRWATCH will need single-photon focal-plane detectors of a million pixels in a design which is optimized to the focusing optics and electronics at acceptable cost. We discuss different phototube possibilities and their pros and cons with crude cost estimates. We conclude that a multichannel-photomultiplier solution is safe. A better compromise may be to adapt a 6 or 9 inch X-ray image intensifier tube or develop a 12 inch image intensifier for detecting individual photons, and adapt the optics to have many mirror modules. The possibility of developing super-large-area phototubes is also discussed.

  4. A comparison of blue light and caffeine effects on cognitive function and alertness in humans.

    PubMed

    Beaven, C Martyn; Ekström, Johan

    2013-01-01

    The alerting effects of both caffeine and short wavelength (blue) light have been consistently reported. The ability of blue light to enhance alertness and cognitive function via non-image forming neuropathways have been suggested as a non-pharmacological countermeasure for drowsiness across a range of occupational settings. Here we compare and contrast the alerting and psychomotor effects of 240 mg of caffeine and a 1-h dose of ~40 lx blue light in a non-athletic population. Twenty-one healthy subjects performed a computer-based psychomotor vigilance test before and after each of four randomly assigned trial conditions performed on different days: white light/placebo; white light/240 mg caffeine; blue light/placebo; blue light/240 mg caffeine. The Karolinska Sleepiness Scale was used to assess subjective measures of alertness. Both the caffeine only and blue light only conditions enhanced accuracy in a visual reaction test requiring a decision and an additive effect was observed with respect to the fastest reaction times. However, in a test of executive function, where a distraction was included, caffeine exerted a negative effect on accuracy. Furthermore, the blue light only condition consistently outperformed caffeine when both congruent and incongruent distractions were presented. The visual reactions in the absence of a decision or distraction were also enhanced in the blue light only condition and this effect was most prominent in the blue-eyed participants. Overall, blue light and caffeine demonstrated distinct effects on aspects of psychomotor function and have the potential to positively influence a range of settings where cognitive function and alertness are important. Specifically, despite the widespread use of caffeine in competitive sporting environments, the possible impact of blue light has received no research attention.

  5. Platinum blue as an alternative to uranyl acetate for staining in transmission electron microscopy.

    PubMed

    Inaga, Sumire; Katsumoto, Tetsuo; Tanaka, Keiichi; Kameie, Toshio; Nakane, Hironobu; Naguro, Tomonori

    2007-04-01

    This paper introduces an aqueous solution of platinum blue (Pt-blue) as an alternative to uranyl acetate (UA) for staining in transmission electron microscopy (TEM). Pt-blue was prepared from a reaction of cis-dichlorodiamine-platinum (II) (cis-platin) with thymidine. When Pt-blue was dried on a microgrid and observed by TEM it showed a uniform appearance with tiny particles less than 1 nm in diameter. The effect of Pt-blue as an electron stain was then examined not only for positive staining of conventional ultrathin resin sections and counterstaining of post-embedding immuno-electron microscopy but also for negative staining. In ultrathin sections of the rat liver and renal glomerulus, Pt-blue provided good contrast images, especially in double staining combined with a lead stain (Pb). Almost all cell organelles were clearly observed with high contrast in these sections. Glycogen granules in the hepatic parenchymal cells were particularly electron dense in Pt-blue stained sections compared with those treated with UA. In longitudinal and transverse sections of budding influenza A viruses, a specific arrangement of rod-like structures, which correspond to the ribonucleoprotein complexes, was clearly shown in each virion stained with Pt-blue and Pb. When post-embedding immunoelectron microscopy was performed in ultrathin sections of HeLa cells embedded in Lowicryl K4M, the localization of Ki-67 protein was sufficiently detected even after Pt-blue and Pb staining. The present study also revealed that Pt-blue could be used for the negative staining of E. coli, allowing the visualization of a flagellum. These findings indicate that Pt-blue is a useful, safe, and easily obtainable electron stain that is an alternative to UA for TEM preparations.

  6. A Comparison of Blue Light and Caffeine Effects on Cognitive Function and Alertness in Humans

    PubMed Central

    Beaven, C. Martyn; Ekström, Johan

    2013-01-01

    The alerting effects of both caffeine and short wavelength (blue) light have been consistently reported. The ability of blue light to enhance alertness and cognitive function via non-image forming neuropathways have been suggested as a non-pharmacological countermeasure for drowsiness across a range of occupational settings. Here we compare and contrast the alerting and psychomotor effects of 240 mg of caffeine and a 1-h dose of ~40 lx blue light in a non-athletic population. Twenty-one healthy subjects performed a computer-based psychomotor vigilance test before and after each of four randomly assigned trial conditions performed on different days: white light/placebo; white light/240 mg caffeine; blue light/placebo; blue light/240 mg caffeine. The Karolinska Sleepiness Scale was used to assess subjective measures of alertness. Both the caffeine only and blue light only conditions enhanced accuracy in a visual reaction test requiring a decision and an additive effect was observed with respect to the fastest reaction times. However, in a test of executive function, where a distraction was included, caffeine exerted a negative effect on accuracy. Furthermore, the blue light only condition consistently outperformed caffeine when both congruent and incongruent distractions were presented. The visual reactions in the absence of a decision or distraction were also enhanced in the blue light only condition and this effect was most prominent in the blue-eyed participants. Overall, blue light and caffeine demonstrated distinct effects on aspects of psychomotor function and have the potential to positively influence a range of settings where cognitive function and alertness are important. Specifically, despite the widespread use of caffeine in competitive sporting environments, the possible impact of blue light has received no research attention. PMID:24282477

  7. Multispectral Focal Plane Assembly for Satellite Remote Sensing

    SciTech Connect

    Rienstra, J.; Ballard, M.

    1997-12-31

    Sandia National Laboratories and several subsystem contractors are developing technologies applicable to multispectral remote sensing from space. A proof of concept multispectral sensor system is under development. The objective of building this sensor is to demonstrate and evaluate multispectral imaging technologies for various applications. The three major subsystems making up the sensor are the focal plane assembly (FPA), the cryocooler, and the telescope. This paper covers the focal plane assembly, which is the basis of the sensor system. The focal plane assembly includes sensor chip assemblies, optical filters, and a vacuum enclosure with cold shielding. Linear detector arrays provide spatial resolution in the cross-track direction for a pushbroom imager configuration. The optical filters define 15 spectral bands in a range from 0.45 microns to 10.7 microns. All the detector arrays are mounted on a single focal plane and are designed to operate at 75 K. No beam splitters are used. The four spectral bands covering the visible to near infrared have roughly 2400 pixels each, and the remaining 11 spectral bands have roughly 600 pixels each. The average total rate of multispectral data from the FPA is approximately 15.4 megapixels per second. At the time this paper is being written, the multispectral focal plane assembly is in the fabrication phase. A thermal/mechanical mockup has been built and tested for the vibration environment and to determine the thermal load. Some of the sensor chip assemblies and filters have been built and tested. Several notable features of the design are covered in the paper as well as preliminary test data.

  8. Blue straggler stars: lessons from open clusters.

    NASA Astrophysics Data System (ADS)

    Geller, Aaron M.

    Open clusters enable a deep dive into blue straggler characteristics. Recent work shows that the binary properties (frequency, orbital elements and companion masses and evolutionary states) of the blue stragglers are the most important diagnostic for determining their origins. To date the multi-epoch radial-velocity observations necessary for characterizing these blue straggler binaries have only been carried out in open clusters. In this paper, I highlight recent results in the open clusters NGC 188, NGC 2682 (M67) and NGC 6819. The characteristics of many of the blue stragglers in these open clusters point directly to origins through mass transfer from an evolved donor star. Additionally, a handful of blue stragglers show clear signatures of past dynamical encounters. These comprehensive, diverse and detailed observations also reveal important challenges for blue straggler formation models (and particularly the mass-transfer channel), which we must overcome to fully understand the origins of blue straggler stars and other mass-transfer products.

  9. Localized Eruptive Blue Nevi after Herpes Zoster

    PubMed Central

    Colson, Fany; Arrese, Jorge E.; Nikkels, Arjen F.

    2016-01-01

    A 52-year-old White man presented with a dozen small, well-restricted, punctiform, asymptomatic, blue-gray macules on the left shoulder. A few months earlier, he had been treated with oral acyclovir for herpes zoster (HZ) affecting the left C7–C8 dermatomes. All the blue macules appeared over a short period of time and then remained stable. The patient had not experienced any previous trauma or had tattooing in this anatomical region. The clinical diagnosis suggested blue nevi. Dermatoscopy revealed small, well-limited, dark-blue, compact, homogeneous areas evoking dermal blue nevi. An excisional biopsy was performed and the histological examination confirmed a blue nevus. As far as we are aware of, this is the first report of eruptive blue nevi following HZ, and it should be included in the differential diagnosis of zosteriform dermatoses responding to an isotopic pathway. In addition, a brief review concerning eruptive nevi is presented. PMID:27462219

  10. Out-of-plane properties

    SciTech Connect

    Jackson, W.C.; Portanova, M.A.

    1995-10-01

    This paper summarizes three areas of research which were performed to characterize out-of-plane properties of composite materials. In the first investigation, a series of tests was run to characterize the through-the-thickness tensile strength for a variety of composites that included 2D braids, 2D and 3D weaves, and prepreg tapes. A new test method based on a curved beam was evaluated. Failures were significantly different between the 2D materials and the 3D weaves. The 2D materials delaminated between layers due to out-of-plane tensile stresses while the 3D weaves failed due to the formation of radial cracks between the surface plies caused by high circumferential stresses along the inner radius. The strength of the 2D textile composites did not increase relative to the tapes. Final failure in the 3D weaves was caused by a circumferential crack similar to the 2D materials and occurred at a lower bending moment than in other materials. The early failures in the 3D weaves were caused by radial crack formation rather than a low through-the-thickness strength. The second investigation focused on the development of a standard impact test method to measure impact damage resistance. The only impact tests that currently exist are compression after impact (CAI) tests which incorporate elements of both damage resistance and damage tolerance. A new impact test method is under development which uses a quasi-static indentation (QSI) test to directly measure damage resistance. Damage resistance is quantified in terms of the contact force to produce a unit of damage where a metric for damage may be area in C-scan, depth of residual dent, penetration, damage growth, etc. A final draft of an impact standard that uses a QSI test method will be presented to the ASTM Impact Task Group on impact. In the third investigation, the impact damage resistance behavior of a variety of textile materials was studied using the QSI test method.

  11. The UKIDSS Galactic Plane Survey

    NASA Astrophysics Data System (ADS)

    Lucas, P. W.; Hoare, M. G.; Longmore, A.; Schröder, A. C.; Davis, C. J.; Adamson, A.; Bandyopadhyay, R. M.; de Grijs, R.; Smith, M.; Gosling, A.; Mitchison, S.; Gáspár, A.; Coe, M.; Tamura, M.; Parker, Q.; Irwin, M.; Hambly, N.; Bryant, J.; Collins, R. S.; Cross, N.; Evans, D. W.; Gonzalez-Solares, E.; Hodgkin, S.; Lewis, J.; Read, M.; Riello, M.; Sutorius, E. T. W.; Lawrence, A.; Drew, J. E.; Dye, S.; Thompson, M. A.

    2008-11-01

    The UKIDSS Galactic Plane Survey (GPS) is one of the five near-infrared Public Legacy Surveys that are being undertaken by the UKIDSS consortium, using the Wide Field Camera on the United Kingdom Infrared Telescope. It is surveying 1868 deg2 of the northern and equatorial Galactic plane at Galactic latitudes -5° < b < 5° in the J, H and K filters and a ~200-deg2 area of the Taurus-Auriga-Perseus molecular cloud complex in these three filters and the 2.12 μm (1-0) H2 filter. It will provide data on ~2 × 109 sources. Here we describe the properties of the data set and provide a user's guide for its exploitation. We also present brief Demonstration Science results from DR2 and from the Science Verification programme. These results illustrate how GPS data will frequently be combined with data taken in other wavebands to produce scientific results. The Demonstration Science comprises six studies. (1) A GPS-Spitzer-GLIMPSE cross-match for the star formation region G28.983-0.603 to identify YSOs. This increases the number of YSOs identified by a factor of 10 compared to GLIMPSE alone. (2) A wide-field study of the M17 nebula, in which an extinction map of the field is presented and the effect of source confusion on luminosity functions in different subregions is noted. (3) H2 emission in the ρ Ophiuchi dark cloud. All the molecular jets are traced back to a single active clump containing only a few protostars, which suggests that the duration of strong jet activity and associated rapid accretion in low-mass protostars is brief. (4) X-ray sources in the nuclear bulge. The GPS data distinguishes local main-sequence counterparts with soft X-ray spectra from nuclear bulge giant counterparts with hard X-ray spectra. (5) External galaxies in the zone of avoidance. The galaxies are clearly distinguished from stars in fields at longitudes l > 90°. (6) IPHAS-GPS optical-infrared spectrophotometric typing. The (i' - J) versus (J - H) diagram is used to distinguish A-F type

  12. Out-of-plane properties

    NASA Technical Reports Server (NTRS)

    Jackson, Wade C.; Portanova, Marc A.

    1995-01-01

    This paper summarizes three areas of research which were performed to characterize out-of-plane properties of composite materials. In the first investigation, a series of tests was run to characterize the through-the-thickness tensile strength for a variety of composites that included 2D braids, 2D and 3D weaves, and prepreg tapes. A new test method based on a curved beam was evaluated. Failures were significantly different between the 2D materials and the 3D weaves. The 2D materials delaminated between layers due to out-of-plane tensile stresses while the 3D weaves failed due to the formation of radial cracks between the surface plies caused by high circumferential stresses along the inner radius. The strength of the 2D textile composites did not increase relative to the tapes. Final failure in the 3D weaves was caused by a circumferential crack similar to the 2D materials and occurred at a lower bending moment than in other materials. The early failures in the 3D weaves were caused by radial crack formation rather than a low through-the-thickness strength. The second investigation focused on the development of a standard impact test method to measure impact damage resistance. The only impact tests that currently exist are compression after impact (CAI) tests which incorporate elements of both damage resistance and damage tolerance. A new impact test method is under development which uses a quasi-static indentation (QSI) test to directly measure damage resistance. Damage resistance is quantified in terms of the contact force to produce a unit of damage where a metric for damage may be area in C-scan, depth of residual dent , penetration, damage growth, etc. A final draft of an impact standard that uses a QSI test method will be presented to the ASTM Impact Task Group on impact. In the third investigation, the impact damage resistance behavior of a variety of textile materials was studied using the QSI test method. In this study, the force where large damage

  13. 2-tier in-plane motion correction and out-of-plane motion filtering for contrast-enhanced ultrasound

    PubMed Central

    Ta, Casey N.; Eghtedari, Mohammad; Mattrey, Robert F.; Kono, Yuko; Kummel, Andrew C.

    2014-01-01

    Objectives Contrast-enhanced ultrasound (CEUS) cines of focal liver lesions (FLL) can be quantitatively analyzed to measure tumor perfusion on a pixel-by-pixel basis for diagnostic indication. However, CEUS cines acquired freehand and during free breathing cause non-uniform in-plane and out-of-plane motion from frame to frame. These motions create fluctuations in the time-intensity curves (TIC), reducing accuracy of quantitative measurements. Out-of-plane motion cannot be corrected by image registration in 2D CEUS and degrades the quality of in-plane motion correction (IPMC). A 2-tier IPMC strategy and adaptive out-of-plane motion filter (OPMF) are proposed to provide a stable correction of non-uniform motion to reduce the impact of motion on quantitative analyses. Materials and Methods 22 cines of FLLs were imaged with dual B-mode and contrast specific imaging to acquire a 3-minute TIC. B-mode images were analyzed for motion, and the motion correction was applied to both B-mode and contrast images. For IPMC, the main reference frame was automatically selected for each cine, and subreference frames were selected in each respiratory cycle and sequentially registered toward the main reference frame. All other frames were sequentially registered toward the local subreference frame. Four OPMFs were developed and tested: subsample Normalized Correlation (NC), subsample Sum of Absolute Differences (SAD), mean frame NC, and histogram. The frames that were most dissimilar to the OPMF reference frame using one of the four above criteria in each respiratory cycle were adaptively removed by thresholding against the low-pass filter of the similarity curve. OPMF was quantitatively evaluated by an out-of-plane motion metric (OPMM) that measured normalized variance in the high-pass filtered time-intensity curve within the tumor region-of-interest with low OPMM being the goal. IPMC and OPMF results were qualitatively evaluated by two blinded observers who ranked the motion in the

  14. Polish Terms for "Blue" in the Perspective of Vantage Theory

    ERIC Educational Resources Information Center

    Stanulewicz, Danuta

    2010-01-01

    The Polish set of terms for blue includes, inter alia, the following adjectives: "niebieski" "blue", "blekitny" "(sky) blue", "granatowy" "navy blue", "lazurowy" "azure", "modry" "(intense) blue" and "siny" "(grey) violet-blue". The adjective "niebieski" is the basic term; however, it shares some of its functions with "blekitny", which is…

  15. Galactic plane gamma-radiation

    NASA Technical Reports Server (NTRS)

    Hartman, R. C.; Kniffen, D. A.; Thompson, D. J.; Fichtel, C. E.; Ogelman, H. B.; Tumer, T.; Ozel, M. E.

    1979-01-01

    Analysis of the SAS 2 data together with the COS B results shows that the distribution of galactic gamma-radiation has several similarities to that of other large-scale tracers of galactic structure. The radiation is primarily confined to a thin disc which exhibits offsets from b = 0 degrees similar to warping at radio frequencies. The principal distinction of the gamma-radiation is a stronger contrast in intensity between the region from 310 to 45 degrees in longitude and the regions away from the center that can be attributed to a variation in cosmic-ray density as a function of position in Galaxy. The diffuse galactic gamma-ray energy spectrum shows no significant variation in direction, and the spectrum seen along the plane is the same as that for the galactic component of the gamma-radiation at high altitudes. The uniformity of the galactic gamma-ray spectrum, the smooth decrease in intensity as a function of altitude, and the absence of any galactic gamma-ray sources at high altitudes indicate a diffuse origin for bulk of the galactic gamma-radiation rather than a collection of localized sources.

  16. Duel-Plane Optical Disdrometer

    NASA Astrophysics Data System (ADS)

    Winsky, B. E.; Eichinger, W. E.

    2011-12-01

    Acquiring better drop-size distributions of rainfall will improve our understanding of the spatial and temporal variability of rainfall. In order to fully capture the spatial and temporal variability of rainfall, a robust, calibration free, low-cost instrument that provides an accurate drop-size distribution is required. Therefore, The University of Iowa Lidar Group has developed and built a new duel-plane optical disdrometer that meets these criteria. Two sheets of laser light, vertically spaced by 1 cm are produced by two 670nm laser beams passing through a collecting lens and culminating lens, respectively. The two sheets of laser light then pass through a convex lens located 20 cm from the lasers that focuses the light on a photo detector. A computer reads in and stores the voltages at 10 kHz. The velocity, diameter, shape and drop-size distribution of raindrops are extracted from the voltage measurements. Rainfall data collected in Iowa City, IA tested our disdrometer's robustness and accuracy of providing drop-size distributions. Our distrometer is advantageous because it is simple, low-cost, and requires no calibration.

  17. Radioactivity in the galactic plane

    NASA Technical Reports Server (NTRS)

    Walraven, G. D.; Haymes, R. C.

    1976-01-01

    The paper reports the detection of a large concentration of interstellar radioactivity during balloon-altitude measurements of gamma-ray energy spectra in the band between 0.02 and 12.27 MeV from galactic and extragalactic sources. Enhanced counting rates were observed in three directions towards the plane of the Galaxy; a power-law energy spectrum is computed for one of these directions (designated B 10). A large statistical deviation from the power law in a 1.0-FWHM interval centered near 1.16 MeV is discussed, and the existence of a nuclear gamma-ray line at 1.15 MeV in B 10 is postulated. It is suggested that Ca-44, which emits gamma radiation at 1.156 MeV following the decay of radioactive Sc-44, is a likely candidate for this line, noting that Sc-44 arises from Ti-44 according to explosive models of supernova nucleosynthesis. The 1.16-MeV line flux inferred from the present data is shown to equal the predicted flux for a supernova at a distance of approximately 3 kpc and an age not exceeding about 100 years.

  18. On plane submerged laminar jets

    NASA Astrophysics Data System (ADS)

    Coenen, Wilfried; Sanchez, Antonio L.

    2016-11-01

    We address the laminar flow generated when a developed stream of liquid of kinematic viscosity ν flowing along channel of width 2 h discharges into an open space bounded by two symmetric plane walls departing from the channel rim with an angle α 1 . Attention is focused on values of the jet volume flux 2 Q such that the associated Reynolds number Re = Qh / ν is of order unity. The formulation requires specification of the boundary conditions far from the channel exit. If the flow is driven by the volume flux, then the far-field solution corresponds to Jeffery-Hamel self-similar flow. However, as noted by Fraenkel (1962), such solutions exist only for α <129o in a limited range of Reynolds numbers 0 <=Re <=Rec (α) (e.g. Rec = 1 . 43 for α = π / 2). It is reasoned that an alternative solution, driven by a fraction of the momentum flux of the feed stream, may also exist for all values of Re and α, including a near-centerline Bickley jet, a surrounding Taylor potential flow driven by the jet entrainment, and a Falkner-Skan near-wall boundary layer. Numerical integrations of the Navier-Stokes equations are used to ascertain the existence of these different solutions.

  19. Adsorption of Methylene Blue, Bromophenol Blue, and Coomassie Brilliant Blue by α-chitin nanoparticles

    PubMed Central

    Dhananasekaran, Solairaj; Palanivel, Rameshthangam; Pappu, Srinivasan

    2015-01-01

    Expelling of dyestuff into water resource system causes major thread to the environment. Adsorption is the cost effective and potential method to remove the dyes from the effluents. Therefore, an attempt was made to study the adsorption of dyestuff (Methylene Blue (MB), Bromophenol Blue (BPB) and Coomassie Brilliant Blue (CBB)) by α-chitin nanoparticles (CNP) prepared from Penaeus monodon (Fabricius, 1798) shell waste. On contrary to the most recognizable adsorption studies using chitin, this is the first study using unique nanoparticles of ⩽50 nm used for the dye adsorption process. The results showed that the adsorption process increased with increase in the concentration of CNP, contact time and temperature with the dyestuff, whereas the adsorption process decreased with increase in the initial dye concentration and strong acidic pH. The results from Fourier transform infrared (FTIR) spectroscopy confirmed that the interaction between dyestuff and CNP involved physical adsorption. The adsorption process obeys Langmuir isotherm (R2 values were 0.992, 0.999 and 0.992 for MB, BPB and CBB, and RL value lies between 0 and 1 for all the three dyes) and pseudo second order kinetics (R2 values were 0.996, 0.999 and 0.996 for MB, BPB and CBB) more effectively. The isotherm and kinetic models confirmed that CNP can be used as a suitable adsorbent material for the removal of dyestuff from effluents. PMID:26843977

  20. Adsorption of Methylene Blue, Bromophenol Blue, and Coomassie Brilliant Blue by α-chitin nanoparticles.

    PubMed

    Dhananasekaran, Solairaj; Palanivel, Rameshthangam; Pappu, Srinivasan

    2016-01-01

    Expelling of dyestuff into water resource system causes major thread to the environment. Adsorption is the cost effective and potential method to remove the dyes from the effluents. Therefore, an attempt was made to study the adsorption of dyestuff (Methylene Blue (MB), Bromophenol Blue (BPB) and Coomassie Brilliant Blue (CBB)) by α-chitin nanoparticles (CNP) prepared from Penaeus monodon (Fabricius, 1798) shell waste. On contrary to the most recognizable adsorption studies using chitin, this is the first study using unique nanoparticles of ⩽50 nm used for the dye adsorption process. The results showed that the adsorption process increased with increase in the concentration of CNP, contact time and temperature with the dyestuff, whereas the adsorption process decreased with increase in the initial dye concentration and strong acidic pH. The results from Fourier transform infrared (FTIR) spectroscopy confirmed that the interaction between dyestuff and CNP involved physical adsorption. The adsorption process obeys Langmuir isotherm (R (2) values were 0.992, 0.999 and 0.992 for MB, BPB and CBB, and RL value lies between 0 and 1 for all the three dyes) and pseudo second order kinetics (R (2) values were 0.996, 0.999 and 0.996 for MB, BPB and CBB) more effectively. The isotherm and kinetic models confirmed that CNP can be used as a suitable adsorbent material for the removal of dyestuff from effluents.