Nanomaterials from bacterial cellulose for antimicrobial wound dressing

NASA Astrophysics Data System (ADS)

Liyaskina, E.; Revin, V.; Paramonova, E.; Nazarkina, M.; Pestov, N.; Revina, N.; Kolesnikova, S.

2017-01-01

Bacterial nanocellulose (BNC) is widely used in biomedical applications. BNC has attracted increasing attention as a novel wound dressing material, but it has no antimicrobial activity. To get over this problem in the present study the BNC was saturated with antibiotic fusidic acid (FA). The subject of the experiment was BNC, produced by bacteria Gluconacetobacter sucrofermentans B-11267. The resulting biocomposites have high antibiotic activity against Staphylococcus aureus and can be used in medicine as a wound dressing. The structure of BNC was analyzed by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR).

Cryogenic Feedthrough Test Rig

NASA Technical Reports Server (NTRS)

Skaff, Antony

2009-01-01

The cryogenic feedthrough test rig (CFTR) allows testing of instrumentation feedthroughs at liquid oxygen and liquid hydrogen temperature and pressure extremes (dangerous process fluid) without actually exposing the feedthrough to a combustible or explosive process fluid. In addition, the helium used (inert gas), with cryogenic heat exchangers, exposes the feedthrough to that environment that allows definitive leak rates of feedthrough by typical industry-standard helium mass spectrometers.

HIV-1 Therapy with Monoclonal Antibody 3BNC117 Elicits Host Immune Responses against HIV-1

PubMed Central

Schoofs, Till; Klein, Florian; Braunschweig, Malte; Kreider, Edward F.; Feldmann, Anna; Nogueira, Lilian; Oliveira, Thiago; Lorenzi, Julio C. C.; Parrish, Erica H.; Learn, Gerald H.; West, Anthony P.; Bjorkman, Pamela J.; Schlesinger, Sarah J.; Seaman, Michael S.; Czartoski, Julie; McElrath, M. Juliana; Pfeifer, Nico; Hahn, Beatrice H.; Caskey, Marina; Nussenzweig, Michel C.

2016-01-01

3BNC117 is a broad and potent anti-HIV-1 neutralizing antibody that targets the CD4 binding site on the viral envelope spike. When administered passively, this antibody can prevent infection in animal models and suppress viremia in HIV-1-infected individuals. Here we report that HIV-1 immunotherapy with a single injection of 3BNC117 impacts host antibody responses in viremic subjects. In comparison to untreated controls that showed little change in their neutralizing activity over a six-month period, 3BNC117 infusion significantly improved neutralizing responses to heterologous tier 2 viruses in nearly all study participants. We conclude that 3BNC117-mediated immunotherapy enhances host humoral immunity to HIV-1. PMID:27199429

Synthesis and characterization of a novel polyborosilazane for SiBNC ceramic

NASA Astrophysics Data System (ADS)

Zhang, C. Y.; Liu, Y.; Han, K. Q.; Chang, X. F.; Yu, M. H.

2018-05-01

A novel polyborosilazane (PBSZ) for preparing SiBNC ceramics was successfully synthesized via co-condensation approach using tetrachlorosilan (SiCl4), trichloride (BCl3) and propylamine (C3H7NH2) as starting materials. After pyrolysis of these precursors, amorphous SiBNC ceramics were obtained. The chemical composition, structure and thermal stability of the synthesized PBSZ precursor and SiBNC ceramics were analyzed by using FT-IR, NMR, TGA and XRD methods. The results indicated that the PBSZ contained the major framework of –Si-N-B- and six-membered boron-nitrogen rings. The PBSZ precursor had an approximately ceramic yield of 63 wt% prolyzed at 900°C in nitrogen atmosphere. The SiBNC ceramics shows excellent oxidation resistance and maintained amorphous up to 1600°C.

Active wound dressings based on bacterial nanocellulose as drug delivery system for octenidine.

PubMed

Moritz, Sebastian; Wiegand, Cornelia; Wesarg, Falko; Hessler, Nadine; Müller, Frank A; Kralisch, Dana; Hipler, Uta-Christina; Fischer, Dagmar

2014-08-25

Although bacterial nanocellulose (BNC) may serve as an ideal wound dressing, it exhibits no antibacterial properties by itself. Therefore, in the present study BNC was functionalized with the antiseptic drug octenidine. Drug loading and release, mechanical characteristics, biocompatibility, and antimicrobial efficacy were investigated. Octenidine release was based on diffusion and swelling according to the Ritger-Peppas equation and characterized by a time dependent biphasic release profile, with a rapid release in the first 8h, followed by a slower release rate up to 96 h. The comparison between lab-scale and up-scale BNC identified thickness, water content, and the surface area to volume ratio as parameters which have an impact on the control of the release characteristics. Compression and tensile strength remained unchanged upon incorporation of octenidine in BNC. In biological assays, drug-loaded BNC demonstrated high biocompatibility in human keratinocytes and antimicrobial activity against Staphylococcus aureus. In a long-term storage test, the octenidine loaded in BNC was found to be stable, releasable, and biologically active over a period of 6 months without changes. In conclusion, octenidine loaded BNC presents a ready-to-use wound dressing for the treatment of infected wounds that can be stored over 6 months without losing its antibacterial activity. Copyright © 2014 Elsevier B.V. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hu Jing; Rovey, Joshua L.

A movable Faraday cup design with simple structure and adjustable impedance is described in this work. This Faraday cup has external adjustable shunt resistance for self-biased measurement setup and 50 {Omega} characteristic impedance to match with 50 {Omega} standard BNC coaxial cable and vacuum feedthroughs for nanosecond-level pulse signal measurements. Adjustable shunt resistance allows self-biased measurements to be quickly acquired to determine the electron energy distribution function. The performance of the Faraday cup is validated by tests of response time and amplitude of output signal. When compared with a reference source, the percent difference of the Faraday cup signal fallmore » time is less than 10% for fall times greater than 10 ns. The percent difference of the Faraday cup signal pulse width is below 6.7% for pulse widths greater than 10 ns. A pseudospark-generated electron beam is used to compare the amplitude of the Faraday cup signal with a calibrated F-70 commercial current transformer. The error of the Faraday cup output amplitude is below 10% for the 4-14 kV tested pseudospark voltages. The main benefit of this Faraday cup is demonstrated by adjusting the external shunt resistance and performing the self-biased method for obtaining the electron energy distribution function. Results from a 4 kV pseudospark discharge indicate a ''double-humped'' energy distribution.« less

Hermetic electronics package with dual-sided electrical feedthrough configuration

DOEpatents

Shah, Kedar G.; Pannu, Satinderpall S.

2016-11-22

A hermetic electronics package includes a metal case with opposing first and second open ends, with each end connected to a first feedthrough construction and a second feedthrough construction. Each feedthrough contruction has an electrically insulating substrate and an array of electrically conductive feedthroughs extending therethrough, with the electrically insulating substrates connected to the opposing first and second open ends, respectively, of the metal case so as to form a hermetically sealed enclosure. A set of electronic components are located within the hermetically sealed enclosure and are operably connected to the feedthroughs of the first and second feedthrough constructions so as to electrically communicate outside the package from opposite sides of the package.

Bladder neck contracture after robot-assisted laparoscopic radical prostatectomy: evaluation of incidence and risk factors and impact on urinary function.

PubMed

Msezane, Lambda P; Reynolds, W Stuart; Gofrit, Ofer N; Shalhav, Arieh L; Zagaja, Gregory P; Zorn, Kevin C

2008-01-01

Bladder neck contracture (BNC) after radical prostatectomy has been reported to occur in 5% to 32% of men after open retropubic prostatectomy (RRP) and in 0% to 3% after laparoscopic RRP. Optimal anastomotic closure involves creating a watertight, tension-free anastomosis with well-vascularized, mucosal apposition and correct realignment of the urethra. The cause of BNC is poorly understood; however, it is likely related to multiple factors, including excessive luminal narrowing at the site of reconstruction, local tissue ischemia, failed mucosal apposition, and urinary leakage. In this large series of patients who underwent robot-assisted laparoscopic radical prostatectomy (RLRP), we report the incidence of BNC, evaluate the influence of age, body mass index (BMI), estimated blood loss (EBL), surgical time, and prostate weight on its development and assess follow-up urinary function. Between February 2003 and July 2006, 650 consecutive men underwent RLRP at our institution. Patients with aborted or open conversion procedures were excluded from analysis. The mean overall follow-up for the remaining 634 patients was 19.5 months. Patients presenting with symptoms of outlet obstruction were evaluated with cystoscopy to confirm a BNC. Comparisons of age, BMI, EBL, operative time, and prostate weight were performed using the Student t-test and chi-square analysis. BNC was the diagnosis in seven patients (1.1%) with a mean time of presentation of 4.8 (3-12) months postoperatively. The BNC patients had comparable mean age, BMI, prostate weight, and EBL to the non-BNC cohort. Their operative time, however, was significantly longer (283 v 225 min., P = 0.04). The incidence of BNC after radical prostatectomy is 2.2% in a large series of men undergoing RLRP. The diagnosis was made within 1 year. No significant impact on urinary continence or quality-of-life urinary function was observed after BNC management. A running anastomosis, better visualization, improved instrument maneuverability, and decreased blood loss may account for such a low rate.

Bladder neck contracture after robot-assisted laparoscopic radical prostatectomy: evaluation of incidence and risk factors and impact on urinary function.

PubMed

Msezane, Lambda P; Reynolds, W Stuart; Gofrit, Ofer N; Shalhav, Arieh L; Zagaja, Gregory P; Zorn, Kevin C

2008-02-01

Bladder neck contracture (BNC) after radical prostatectomy has been reported to occur in 5% to 32% of men after open radical retropubic prostatectomy (RRP) and 0% to 3% after laparoscopic RRP. Optimal anastomotic closure involves creating a watertight, tension-free anastomosis with well-vascularized, mucosal apposition and correct realignment of the urethra. The cause of BNC is poorly understood; however, it is likely related to multiple factors, including excessive luminal narrowing at the site of reconstruction, local tissue ischemia, failed mucosal apposition, and urinary leakage. In this large series of patients who underwent robot-assisted laparoscopic radical prostatectomy (RLRP), we report the incidence of BNC, evaluate the influence of age, body mass index (BMI), estimated blood loss (EBL), surgical time, and prostate weight on its development and assess follow-up urinary function. Between February 2003 and July 2006, 650 consecutive men underwent RLRP at our institution. Patients with aborted or open conversion procedures were excluded from analysis. The mean overall follow-up for the remaining 634 patients was 19.5 months. Patients presenting with symptoms of outlet obstruction were evaluated with cystoscopy to confirm a BNC. Comparisons of age, BMI, EBL, operative time, and prostate weight were performed using the Student t-test and chi-square analysis. BNC was the diagnosis in seven patients (1.1%), with a mean time of presentation of 4.8 (3-12) months postoperatively. The BNC patients had comparable mean age, BMI, prostate weight, and EBL to the non-BNC cohort. Their operative time, however, was significantly longer (283 v 225 min, P = 0.04). The incidence of BNC after radical prostatectomy is 1.1% in a large series of men undergoing RLRP. The diagnosis was made within 1 year. No significant impact on urinary continence or quality-of-life urinary function was observed after BNC management. A running anastomosis, better visualization, improved instrument maneuverability, and decreased blood loss may account for such a low rate.

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

PubMed Central

Arias, Sandra L.; Shetty, Akshath R.; Senpan, Angana; Echeverry-Rendón, Mónica; Reece, Lisa M.; Allain, Jean Paul

2016-01-01

In this study, bacterial nanocellulose (BNC) produced by the bacteria Gluconacetobacter xylinus is synthesized and impregnated in situ with iron oxide nanoparticles (IONP) (Fe3O4) to yield a magnetic bacterial nanocellulose (MBNC). The synthesis of MBNC is a precise and specifically designed multi-step process. Briefly, bacterial nanocellulose (BNC) pellicles are formed from preserved G. xylinus strain according to our experimental requirements of size and morphology. A solution of iron(III) chloride hexahydrate (FeCl3·6H2O) and iron(II) chloride tetrahydrate (FeCl2·4H2O) with a 2:1 molar ratio is prepared and diluted in deoxygenated high purity water. A BNC pellicle is then introduced in the vessel with the reactants. This mixture is stirred and heated at 80 °C in a silicon oil bath and ammonium hydroxide (14%) is then added by dropping to precipitate the ferrous ions into the BNC mesh. This last step allows forming in situ magnetite nanoparticles (Fe3O4) inside the bacterial nanocellulose mesh to confer magnetic properties to BNC pellicle. A toxicological assay was used to evaluate the biocompatibility of the BNC-IONP pellicle. Polyethylene glycol (PEG) was used to cover the IONPs in order to improve their biocompatibility. Scanning electron microscopy (SEM) images showed that the IONP were located preferentially in the fibril interlacing spaces of the BNC matrix, but some of them were also found along the BNC ribbons. Magnetic force microscope measurements performed on the MBNC detected the presence magnetic domains with high and weak intensity magnetic field, confirming the magnetic nature of the MBNC pellicle. Young's modulus values obtained in this work are also in a reasonable agreement with those reported for several blood vessels in previous studies. PMID:27285589

Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo.

PubMed

Martínez Ávila, Héctor; Feldmann, Eva-Maria; Pleumeekers, Mieke M; Nimeskern, Luc; Kuo, Willy; de Jong, Willem C; Schwarz, Silke; Müller, Ralph; Hendriks, Jeanine; Rotter, Nicole; van Osch, Gerjo J V M; Stok, Kathryn S; Gatenholm, Paul

2015-03-01

Tissue engineering provides a promising alternative therapy to the complex surgical reconstruction of auricular cartilage by using ear-shaped autologous costal cartilage. Bacterial nanocellulose (BNC) is proposed as a promising scaffold material for auricular cartilage reconstruction, as it exhibits excellent biocompatibility and secures tissue integration. Thus, this study evaluates a novel bilayer BNC scaffold for auricular cartilage tissue engineering. Bilayer BNC scaffolds, composed of a dense nanocellulose layer joined with a macroporous composite layer of nanocellulose and alginate, were seeded with human nasoseptal chondrocytes (NC) and cultured in vitro for up to 6 weeks. To scale up for clinical translation, bilayer BNC scaffolds were seeded with a low number of freshly isolated (uncultured) human NCs combined with freshly isolated human mononuclear cells (MNC) from bone marrow in alginate and subcutaneously implanted in nude mice for 8 weeks. 3D morphometric analysis showed that bilayer BNC scaffolds have a porosity of 75% and mean pore size of 50 ± 25 μm. Furthermore, endotoxin analysis and in vitro cytotoxicity testing revealed that the produced bilayer BNC scaffolds were non-pyrogenic (0.15 ± 0.09 EU/ml) and non-cytotoxic (cell viability: 97.8 ± 4.7%). This study demonstrates that bilayer BNC scaffolds offer a good mechanical stability and maintain a structural integrity while providing a porous architecture that supports cell ingrowth. Moreover, bilayer BNC scaffolds provide a suitable environment for culture-expanded NCs as well as a combination of freshly isolated NCs and MNCs to form cartilage in vitro and in vivo as demonstrated by immunohistochemistry, biochemical and biomechanical analyses. Copyright © 2014 Elsevier Ltd. All rights reserved.

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles.

PubMed

Arias, Sandra L; Shetty, Akshath R; Senpan, Angana; Echeverry-Rendón, Mónica; Reece, Lisa M; Allain, Jean Paul

2016-05-26

In this study, bacterial nanocellulose (BNC) produced by the bacteria Gluconacetobacter xylinus is synthesized and impregnated in situ with iron oxide nanoparticles (IONP) (Fe3O4) to yield a magnetic bacterial nanocellulose (MBNC). The synthesis of MBNC is a precise and specifically designed multi-step process. Briefly, bacterial nanocellulose (BNC) pellicles are formed from preserved G. xylinus strain according to our experimental requirements of size and morphology. A solution of iron(III) chloride hexahydrate (FeCl3·6H2O) and iron(II) chloride tetrahydrate (FeCl2·4H2O) with a 2:1 molar ratio is prepared and diluted in deoxygenated high purity water. A BNC pellicle is then introduced in the vessel with the reactants. This mixture is stirred and heated at 80 °C in a silicon oil bath and ammonium hydroxide (14%) is then added by dropping to precipitate the ferrous ions into the BNC mesh. This last step allows forming in situ magnetite nanoparticles (Fe3O4) inside the bacterial nanocellulose mesh to confer magnetic properties to BNC pellicle. A toxicological assay was used to evaluate the biocompatibility of the BNC-IONP pellicle. Polyethylene glycol (PEG) was used to cover the IONPs in order to improve their biocompatibility. Scanning electron microscopy (SEM) images showed that the IONP were located preferentially in the fibril interlacing spaces of the BNC matrix, but some of them were also found along the BNC ribbons. Magnetic force microscope measurements performed on the MBNC detected the presence magnetic domains with high and weak intensity magnetic field, confirming the magnetic nature of the MBNC pellicle. Young's modulus values obtained in this work are also in a reasonable agreement with those reported for several blood vessels in previous studies.

Bio-nanocapsule-based scaffold improves the sensitivity and ligand-binding capacity of mammalian receptors on the sensor chip.

PubMed

Iijima, Masumi; Yoshimoto, Nobuo; Niimi, Tomoaki; Maturana, Andrés D; Kuroda, Shun'ichi

2016-06-01

Mammalian receptors are recognized as target molecules for drug discovery, and chemical libraries have been screened for both potential antagonists and agonists mainly by ligand-binding assays using immobilized receptors. A bio-nanocapsule (BNC) of approximately 30 nm that displays a tandem form of the protein A-derived immunoglobulin G (IgG) Fc-binding Z domains (denoted as ZZ-BNC) has been developed for both clustering and oriented immobilization of IgGs on the solid phase of immunosensors. In this study, human IgG1 Fc-fused vascular endothelial growth factor (VEGF) receptor was immobilized through ZZ-BNC on the sensor chip of quartz crystal microbalance (ZZ-BNC-coating). When compared with direct adsorption and protein A-coating, the sensor chip showed higher sensitivity (∽46- and ∽165-fold, respectively) and larger ligand-binding capacity (∽4- and ∽18-fold, respectively). Furthermore, the number of VEGF molecules bound to its receptor increased from 0.20 (direct adsorption) to 2.06 by ZZ-BNC-coating, strongly suggesting that ZZ-BNC reduced the steric hindrance near ligand recognition sites through oriented immobilization. Similarly, the sensitivity and ligand-binding capacity of leptin and prolactin receptors were both enhanced at a level comparable to that observed for the VEGF receptor. Thus, the combination of ZZ-BNC and Fc-fused receptors could significantly improve the function of ligand-binding assays. Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

High-density percutaneous chronic connector for neural prosthetics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shah, Kedar G.; Bennett, William J.; Pannu, Satinderpall S.

2015-09-22

A high density percutaneous chronic connector, having first and second connector structures each having an array of magnets surrounding a mounting cavity. A first electrical feedthrough array is seated in the mounting cavity of the first connector structure and a second electrical feedthrough array is seated in the mounting cavity of the second connector structure, with a feedthrough interconnect matrix positioned between a top side of the first electrical feedthrough array and a bottom side of the second electrical feedthrough array to electrically connect the first electrical feedthrough array to the second electrical feedthrough array. The two arrays of magnetsmore » are arranged to attract in a first angular position which connects the first and second connector structures together and electrically connects the percutaneously connected device to the external electronics, and to repel in a second angular position to facilitate removal of the second connector structure from the first connector structure.« less

Fiber Optic Cable Feedthrough and Sealing

NASA Technical Reports Server (NTRS)

Fan, Robert J.

1998-01-01

A novel fiberoptic hermetic bulkhead feedthrough has been developed which will offer cryogenic sealing at leak rates of 10(exp -11) cc/sec helium. This feedthrough was developed for NASA in response to needs for a hermetically sealed feedthrough which could withstand a range of temperatures from low cryogenic (-196 C), due to liquid fuels and oxidizers, to high temperatures (+200 C) encountered in the proximity of combustion gasses. The development effort will be reported from conceptual design of single and multi-channel feedthrough units with single interconnection interfaces to units with double-ended interconnection interfaces. Various combinations of fiber/buffers are reported with recommendations based on test results. A comprehensive series of environmental and mechanical tests were performed to evaluate the feedthroughs in adverse conditions. Test results are reported including insertion loss, salt spray, sinusoidal vibration, random vibration, mechanical shock, thermal shock and humidity. A second set of feedthrough units was exposed to 3 different types of radiation. Optical transmittance changes during the tests were monitored and leak rate testing was done after each test. State-of-the-art technology in optical fiber feedthroughs constructed with polycrystalline ceramic is presented.

Inexpensive high vacuum feedthroughs.

NASA Technical Reports Server (NTRS)

Gerber, S.; Post, D.

1973-01-01

Description of the use of rigid coaxial cable in the construction of high vacuum coaxial and coaxial push-pull rotary motion feedthroughs. This type of feedthroughs is shown to be extremely cheap and simple to make and modify. It can be used for moderately high voltages and provides a continuous, well shielded, low-noise feedthrough cable in any desired configuration.

Nest-site selection, reproductive ecology and shifts within core-use areas of Black-necked Cranes at the northern limit of the Tibetan Plateau

PubMed Central

An, Bei; Shu, Meilin

2017-01-01

We investigated population dynamics, breeding pairs, breeding habitat selection, nest density, distance between neighboring nests, nest survival, reproductive success, and recruitment rate for Black-necked Cranes (BNC, Grus nigricollis) during 2013–2015 in Yanchiwan National Nature Reserve (YCW), Gansu, China. Numbers of BNC and breeding pairs remained relatively stable at around 140 individuals and 40 pairs. Recruitment rates ranged from 15.7% to 25.8%. The average nest distance was 718.66 ± 430.50 m (2013), 1064.51 ± 323.99 m (2014) and 534.99 ± 195.45 m (2015). Average nest survival rate, hatching success, and breeding success of all 29 nests were 65.56 ± 5.09%, 57.04 ± 6.12% and 32.78% ± 2.55. Water depth, water body area, and distance to land were positively related to nest survival, while disturbance level showed a negative relationship. However, nest site selection of BNC was determined by habitat type, disturbance and water depth. BNC often foraged in mudflats and freshwater marsh but seldom foraged in saline-alkali wet meadows due to food density and quantity in April, the month when BNC choose nest sites. Conservation strategies based on habitats should consider ecological factors that may not be well predicted by nest site selection. Shifts within core-use areas from satellite tracking of BNC demonstrated that maintaining populations demands that conservation areas are large enough to permit breeding BNC changes in space use. Our results are important for conservation management and provide quantitative reproductive data for this species. PMID:28168110

Nest-site selection, reproductive ecology and shifts within core-use areas of Black-necked Cranes at the northern limit of the Tibetan Plateau.

PubMed

Zhang, Lixun; An, Bei; Shu, Meilin; Yang, Xiaojun

2017-01-01

We investigated population dynamics, breeding pairs, breeding habitat selection, nest density, distance between neighboring nests, nest survival, reproductive success, and recruitment rate for Black-necked Cranes (BNC, Grus nigricollis ) during 2013-2015 in Yanchiwan National Nature Reserve (YCW), Gansu, China. Numbers of BNC and breeding pairs remained relatively stable at around 140 individuals and 40 pairs. Recruitment rates ranged from 15.7% to 25.8%. The average nest distance was 718.66 ± 430.50 m (2013), 1064.51 ± 323.99 m (2014) and 534.99 ± 195.45 m (2015). Average nest survival rate, hatching success, and breeding success of all 29 nests were 65.56 ± 5.09%, 57.04 ± 6.12% and 32.78% ± 2.55. Water depth, water body area, and distance to land were positively related to nest survival, while disturbance level showed a negative relationship. However, nest site selection of BNC was determined by habitat type, disturbance and water depth. BNC often foraged in mudflats and freshwater marsh but seldom foraged in saline-alkali wet meadows due to food density and quantity in April, the month when BNC choose nest sites. Conservation strategies based on habitats should consider ecological factors that may not be well predicted by nest site selection. Shifts within core-use areas from satellite tracking of BNC demonstrated that maintaining populations demands that conservation areas are large enough to permit breeding BNC changes in space use. Our results are important for conservation management and provide quantitative reproductive data for this species.

Alignment-enhancing feed-through conductors for stackable silicon-on-sapphire wafers

NASA Technical Reports Server (NTRS)

Anthony, Thomas R. (Inventor)

1983-01-01

Alignment-enhancing electrically conductive feed-through paths are provided for the high-speed low-loss transfer of electrical signals between integrated circuits of a plurality of silicon-on-sapphire bodies arrayed in a stack. The alignment-enhancing feed-throughs are made by a process involving the drilling of holes through the body, double-sided sputtering, electroplating, and the filling of the holes with solder by capillary action. The alignment-enhancing feed-throughs are activated by forming a stack of wafers and remelting the solder whereupon the wafers, and the feed-through paths, are pulled into alignment by surface tension forces.

Faraday cup with nanosecond response and adjustable impedance for fast electron beam characterization.

PubMed

Hu, Jing; Rovey, Joshua L

2011-07-01

A movable Faraday cup design with simple structure and adjustable impedance is described in this work. This Faraday cup has external adjustable shunt resistance for self-biased measurement setup and 50 Ω characteristic impedance to match with 50 Ω standard BNC coaxial cable and vacuum feedthroughs for nanosecond-level pulse signal measurements. Adjustable shunt resistance allows self-biased measurements to be quickly acquired to determine the electron energy distribution function. The performance of the Faraday cup is validated by tests of response time and amplitude of output signal. When compared with a reference source, the percent difference of the Faraday cup signal fall time is less than 10% for fall times greater than 10 ns. The percent difference of the Faraday cup signal pulse width is below 6.7% for pulse widths greater than 10 ns. A pseudospark-generated electron beam is used to compare the amplitude of the Faraday cup signal with a calibrated F-70 commercial current transformer. The error of the Faraday cup output amplitude is below 10% for the 4-14 kV tested pseudospark voltages. The main benefit of this Faraday cup is demonstrated by adjusting the external shunt resistance and performing the self-biased method for obtaining the electron energy distribution function. Results from a 4 kV pseudospark discharge indicate a "double-humped" energy distribution.

Radio frequency coaxial feedthrough device

DOEpatents

Owens, Thomas L.; Baity, Frederick W.; Hoffman, Daniel J.; Whealton, John H.

1987-01-01

A radio frequency coaxial vacuum feedthrough is provided which utilizes a cylindrical ceramic vacuum break formed of an alumina ceramic. The cylinder is coaxially disposed and brazed between tapered coaxial conductors to form a vacuum sealed connection between a pressurized upstream coaxial transmission line and a utilization device located within a vacuum container. The feedthrough provides 50 ohm matched impedance RF feedthrough up to about 500 MHz at power levels in the multimegawatt range.

Method for making alignment-enhancing feed-through conductors for stackable silicon-on-sapphire

NASA Technical Reports Server (NTRS)

Anthony, Thomas R. (Inventor)

1985-01-01

Alignment-enhancing electrically conductive feed-through paths are provided for the high-speed low-loss transfer of electrical signals between integrated circuits of a plurality of silicon-on-sapphire bodies arrayed in a stack. The alignment-enhancing feed-throughs are made by a process of this invention involving the drilling of holes through the body, double-sided sputtering, electroplating, and the filling of the holes with solder by capillary action. The alignment-enhancing feed-throughs are activated by forming a stack of wafers and remelting the solder whereupon the wafers, and the feed-through paths, are pulled into alignment by surface tension forces.

Development of a macrophage-targeting and phagocytosis-inducing bio-nanocapsule-based nanocarrier for drug delivery.

PubMed

Li, Hao; Tatematsu, Kenji; Somiya, Masaharu; Iijima, Masumi; Kuroda, Shun'ichi

2018-06-01

Macrophage hyperfunction or dysfunction is tightly associated with various diseases, such as osteoporosis, inflammatory disorder, and cancers. However, nearly all conventional drug delivery system (DDS) nanocarriers utilize endocytosis for entering target cells; thus, the development of macrophage-targeting and phagocytosis-inducing DDS nanocarriers for treating these diseases is required. In this study, we developed a hepatitis B virus (HBV) envelope L particle (i.e., bio-nanocapsule (BNC)) outwardly displaying a tandem form of protein G-derived IgG Fc-binding domain and protein L-derived IgG Fab-binding domain (GL-BNC). When conjugated with the macrophage-targeting ligand, mouse IgG2a (mIgG2a), the GL-BNC itself, and the liposome-fused GL-BNC (i.e., GL-virosome) spontaneously initiated aggregation by bridging between the Fc-binding domain and Fab-binding domain with mIgG2a. The aggregates were efficiently taken up by macrophages, whereas this was inhibited by latrunculin B, a phagocytosis-specific inhibitor. The mIgG2a-GL-virosome containing doxorubicin exhibited higher cytotoxicity toward macrophages than conventional liposomes and other BNC-based virosomes. Thus, GL-BNCs and GL-virosomes may constitute promising macrophage-targeting and phagocytosis-inducing DDS nanocarriers. We have developed a novel macrophage-targeting and phagocytosis-inducing bio-nanocapsule (BNC)-based nanocarrier named GL-BNC, which comprises a hepatitis B virus envelope L particle outwardly displaying protein G-derived IgG Fc- and protein L-derived IgG Fab-binding domains in tandem. The GL-BNC alone or liposome-fused form (GL-virosomes) could spontaneously aggregate when conjugated with macrophage-targeting IgGs, inducing phagocytosis by the interaction between IgG Fc of aggregates and FcγR on phagocytes. Thereby these aggregates were efficiently taken up by macrophages. GL-virosomes containing doxorubicin exhibited higher cytotoxicity towards macrophages than ZZ-virosomes and liposomes. Our results suggested that GL-BNCs and GL-virosomes would serve as promising drug delivery system nanocarriers for targeting delivery to macrophages. Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Design and Manufacturing Criteria for Beam Position Monitor (BPM) of Taiwan Photon Source (TPS)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hsueh, H. P.; Chang, C. C.; Hsu, S. N.

2010-06-23

There are quite some considerations when the button feedthrough is designed and manufactured in a new 3rd generation synchrotron light source like the to-be-constructed TPS. It is the responsibility of the button feedthrough designer to design a feedthrough with the lowest probable HOM problem. It is also required for the designer to analyze the consequences for each possible mechanical error during manufacturing and therefore set standards of tolerance. We have been using MAFIA to optimize the design of feedthrough including flange type on bending chamber section and primary BPM on insertion device section. We also have set some criteria formore » feedthrough manufacturing, inspection, and welding based on hands-on experience on feedthrough electrical properties measuring, sorting, inspection, and laser welding. Here we will present the MAFIA results, electrical measurement results, and laser welding results and analysis. Criteria based on these results will also be presented.« less

Chip-scale hermetic feedthroughs for implantable bionics.

PubMed

Guenther, Thomas; Dodds, Christopher W D; Lovell, Nigel H; Suaning, Gregg J

2011-01-01

Most implantable medical devices such as cochlear implants and visual prostheses require protection of the stimulating electronics. This is achieved by way of a hermetic feedthrough system which typically features three important attributes: biocompatibility with the human body, device hermeticity and density of feedthrough conductors. On the quest for building a visual neuroprosthesis, a high number of stimulating channels is required. This has encouraged new technologies with higher rates of production yield and further miniaturization. An Al(2)O(3) based feedthrough system has been developed comprising up to 20 platinum feedthroughs per square millimeter. Ceramics substrates are shown to have leak rates below 1 × 10(-12) atm × cc/s, thus exceeding the resolution limits of most commercially available leak detectors. A sheet resistance of 0.05 Ω can be achieved. This paper describes the design, fabrication process and hermeticity testing of high density feedthroughs for use in neuroprosthetic implants.

Design and Manufacturing Criteria for Beam Position Monitor (BPM) of Taiwan Photon Source (TPS)

NASA Astrophysics Data System (ADS)

Hsueh, H. P.; Chang, C. C.; Hsu, S. N.; Huang, I. T.; Chen, Y. B.; Kuan, C. K.; Hsiung, G. Y.; Chen, J. R.

2010-06-01

There are quite some considerations when the button feedthrough is designed and manufactured in a new 3rd generation synchrotron light source like the to-be-constructed TPS. It is the responsibility of the button feedthrough designer to design a feedthrough with the lowest probable HOM problem. It is also required for the designer to analyze the consequences for each possible mechanical error during manufacturing and therefore set standards of tolerance. We have been using MAFIA to optimize the design of feedthrough including flange type on bending chamber section and primary BPM on insertion device section. We also have set some criteria for feedthrough manufacturing, inspection, and welding based on hands-on experience on feedthrough electrical properties measuring, sorting, inspection, and laser welding. Here we will present the MAFIA results, electrical measurement results, and laser welding results and analysis. Criteria based on these results will also be presented.

Compact, compression-free, displaceable, and resealable vacuum feedthrough with built-in strain relief for sensitive components such as optical fibers.

PubMed

Buchholz, B; Ebert, V

2014-05-01

For the direct fiber coupling of small optical measurement cells, we developed a new compact vacuum feedthrough for glass fibers and other similarly shaped objects that are compact and that offer the possibility of adjusting the fiber in longitudinal and in circular direction. The feedthrough assembly avoids compression or torsion on the fiber and thus protects, e.g., highly frangible fiber materials. In the following, we will present a brief simulation of the tightness requirements for low-pressure and low-concentration water vapor measurements and we will explain an integrated concept for a displaceable and self-adjustable, compression-free, compact, ultra-high vacuum, resealable feedthrough with good strain relief. The feedthrough has been successfully tested in a laboratory test facility and in several extractive airborne tunable diode laser absorption spectroscopy hygrometers. The leakage rate of the feedthrough presented here was tested via a helium leak searcher and was quantified further in an 8-week vacuum measurement campaign. The leakage rate is determined to be 0.41 ± 0.04 × 10(-9) hPa l/s, which--to our knowledge--is the first time a leakage rate for such a feedthrough has been quantified.

Capacitive Feedthroughs for Medical Implants

PubMed Central

Grob, Sven; Tass, Peter A.; Hauptmann, Christian

2016-01-01

Important technological advances in the last decades paved the road to a great success story for electrically stimulating medical implants, including cochlear implants or implants for deep brain stimulation. However, there are still many challenges in reducing side effects and improving functionality and comfort for the patient. Two of the main challenges are the wish for smaller implants on one hand, and the demand for more stimulation channels on the other hand. But these two aims lead to a conflict of interests. This paper presents a novel design for an electrical feedthrough, the so called capacitive feedthrough, which allows both reducing the size, and increasing the number of included channels. Capacitive feedthroughs combine the functionality of a coupling capacitor and an electrical feedthrough within one and the same structure. The paper also discusses the progress and the challenges of the first produced demonstrators. The concept bears a high potential in improving current feedthrough technology, and could be applied on all kinds of electrical medical implants, even if its implementation might be challenging. PMID:27660602

Capacitive Feedthroughs for Medical Implants.

PubMed

Grob, Sven; Tass, Peter A; Hauptmann, Christian

2016-01-01

Important technological advances in the last decades paved the road to a great success story for electrically stimulating medical implants, including cochlear implants or implants for deep brain stimulation. However, there are still many challenges in reducing side effects and improving functionality and comfort for the patient. Two of the main challenges are the wish for smaller implants on one hand, and the demand for more stimulation channels on the other hand. But these two aims lead to a conflict of interests. This paper presents a novel design for an electrical feedthrough, the so called capacitive feedthrough, which allows both reducing the size, and increasing the number of included channels. Capacitive feedthroughs combine the functionality of a coupling capacitor and an electrical feedthrough within one and the same structure. The paper also discusses the progress and the challenges of the first produced demonstrators. The concept bears a high potential in improving current feedthrough technology, and could be applied on all kinds of electrical medical implants, even if its implementation might be challenging.

Centrosome Clustering in the Development of Bovine Binucleate Trophoblast Giant Cells.

PubMed

Klisch, Karl; Schraner, Elisabeth M; Boos, Alois

2017-01-01

Binucleate trophoblast giant cells (BNC) are the characteristic feature of the ruminant placenta. During their development, BNC pass through 2 acytokinetic mitoses and become binucleate with 2 tetraploid nuclei. In this study, we investigate the number and location of centrosomes in bovine BNC. Centrosomes typically consist of 2 centrioles surrounded by electron-dense pericentriolar material. Duplication of centrosomes is tightly linked to the cell cycle, which ensures that the number of centrosomes remains constant in proliferating diploid cells. Alterations of the cell cycle, which affect the number of chromosome sets, also affect the number of centrosomes. In this study, we use placentomal tissue from pregnant cows (gestational days 80-230) for immunohistochemical staining of γ-tubulin (n = 3) and transmission electron microscopy (n = 3). We show that mature BNC have 4 centrosomes with 8 centrioles, clustered in the angle between the 2 cell nuclei. During the second acytokinetic mitosis, the centrosomes must be clustered to form the poles of a bipolar spindle. In rare cases, centrosome clustering fails and tripolar mitosis leads to the formation of trinucleate "BNC". Generally, centrosome clustering occurs in polyploid tumor cells, which have an increased number of centrioles, but it is absent in proliferating diploid cells. Thus, inhibition of centrosome clustering in tumor cells is a novel promising strategy for cancer treatment. BNC are a cell population in which centrosome clustering occurs as part of the normal life history. Thus, they might be a good model for the study of the molecular mechanisms of centrosome clustering. © 2016 S. Karger AG, Basel.

An all-diamond, hermetic electrical feedthrough array for a retinal prosthesis.

PubMed

Ganesan, Kumaravelu; Garrett, David J; Ahnood, Arman; Shivdasani, Mohit N; Tong, Wei; Turnley, Ann M; Fox, Kate; Meffin, Hamish; Prawer, Steven

2014-01-01

The interface between medical implants and the human nervous system is rapidly becoming more and more complex. This rise in complexity is driving the need for increasing numbers of densely packed electrical feedthrough to carry signals to and from implanted devices. This is particularly crucial in the field of neural prosthesis where high resolution stimulating or recording arrays near peripheral nerves or in the brain could dramatically improve the performance of these devices. Here we describe a flexible strategy for implementing high density, high count arrays of hermetic electrical feedthroughs by forming conducting nitrogen doped nanocrystalline diamond channels within an insulating polycrystalline diamond substrate. A unique feature of these arrays is that the feedthroughs can themselves be used as stimulating electrodes for neural tissue. Our particular application is such a feedthrough, designed as a component of a retinal implant to restore vision to the blind. The hermeticity of the feedthroughs means that the array can also form part of an implantable capsule which can interface directly with internal electronic chips. The hermeticity of the array is demonstrated by helium leak tests and electrical and electrochemical characterisation of the feedthroughs is described. The nitrogen doped nanocrystalline diamond forming the electrical feedthroughs is shown to be non-cyctotoxic. New fabrication strategies, such as the one described here, combined with the exceptional biostability of diamond can be exploited to generate a range of biomedical implants that last for the lifetime of the user without fear of degradation.

Perineal Hernia Is an Unusual Complication Post Perineal Bladder Neck Closure: A Case Report.

PubMed

Omar, Helmy; Helmy, Tamer E; Hafez, Ashraf T; Dawaba, Mohamed E

2017-03-01

Bladder neck closure (BNC) is the ultimate bladder neck reconstruction. If reconstruction fails, closure must be considered as it gives the highest continence rate. The vast majority of BNCs are performed through an abdominal approach (either transvesical or extravesical approach), but perineal approach remains an option for BNC with considerable success rate. Perineal hernia, which is defined as protrusion of abdominal contents through the perineal defect, is a very rare complication after urologic procedures. We report a case of perineal hernia post perineal BNC. Copyright © 2016 Elsevier Inc. All rights reserved.

Bacterial nanocellulose stimulates mesenchymal stem cell expansion and formation of stable collagen-I networks as a novel biomaterial in tissue engineering.

PubMed

Vielreicher, Martin; Kralisch, Dana; Völkl, Simon; Sternal, Fabian; Arkudas, Andreas; Friedrich, Oliver

2018-06-20

Biomimetic scaffolds are of great interest to tissue engineering (TE) and tissue repair as they support important cell functions. Scaffold coating with soluble collagen-I has been used to achieve better tissue integration in orthopaedy, however, as collagen persistence was only temporary such efforts were limited. Adequate coverage with cell-derived ECM collagen-I would promise great success, in particular for TE of mechanically challenged tissues. Here, we have used label-free, non-invasive multiphoton microscopy (MPM) to characterise bacterial nanocellulose (BNC) - a promising biomaterial for bone TE - and their potency to stimulate collagen-I formation by mesenchymal stem cells (MSCs). BNC fleeces were investigated by Second Harmonic Generation (SHG) imaging and by their characteristic autofluorescence (AF) pattern, here described for the first time. Seeded MSCs adhered fast, tight and very stable, grew to multilayers and formed characteristic, wide-spread and long-lasting collagen-I. MSCs used micron-sized lacunae and cracks on the BNC surface as cell niches. Detailed analysis using a collagen-I specific binding protein revealed a highly ordered collagen network structure at the cell-material interface. In addition, we have evidence that BNC is able to stimulate MSCs towards osteogenic differentiation. These findings offer new options for the development of engineered tissue constructs based on BNC.

Controlled extended octenidine release from a bacterial nanocellulose/Poloxamer hybrid system.

PubMed

Alkhatib, Y; Dewaldt, M; Moritz, S; Nitzsche, R; Kralisch, D; Fischer, D

2017-03-01

Although bacterial nanocellulose (BNC) has been widely investigated in the last 10years as drug delivery system, up to now no long-term controlled release of drugs could be realized. Therefore, the aim of the present work was the development of a BNC-based drug delivery system that provides prolonged retention time for the antiseptic octenidine up to one week with improved mechanical and antimicrobial properties as well as a high biocompatibility. BNC was modified by incorporation of differently concentrated Poloxamers 338 and 407 as micelles and gels that were extensively investigated regarding size, surface charge, and dynamic viscosity. Depending on type and concentration of the Poloxamer, a retarded octenidine release up to one week could be accomplished. Additionally, superior material properties such as high compression stability and water binding could be achieved. The antimicrobial activity of octenidine against Staphylococcus aureus and Pseudomonas aeruginosa was not changed by the use of Poloxamers. Excellent biocompatibility of the Poloxamer loaded BNC could be demonstrated after local administration in a shell-less hen's egg model. In conclusion, a long-term delivery system consisting of BNC and Poloxamer could be developed for octenidine as a ready-to-use system e.g. for long-term dermal wound treatment. Copyright © 2016 Elsevier B.V. All rights reserved.

Improved astigmatic focus error detection method

NASA Technical Reports Server (NTRS)

Bernacki, Bruce E.

1992-01-01

All easy-to-implement focus- and track-error detection methods presently used in magneto-optical (MO) disk drives using pre-grooved media suffer from a side effect known as feedthrough. Feedthrough is the unwanted focus error signal (FES) produced when the optical head is seeking a new track, and light refracted from the pre-grooved disk produces an erroneous FES. Some focus and track-error detection methods are more resistant to feedthrough, but tend to be complicated and/or difficult to keep in alignment as a result of environmental insults. The astigmatic focus/push-pull tracking method is an elegant, easy-to-align focus- and track-error detection method. Unfortunately, it is also highly susceptible to feedthrough when astigmatism is present, with the worst effects caused by astigmatism oriented such that the tangential and sagittal foci are at 45 deg to the track direction. This disclosure outlines a method to nearly completely eliminate the worst-case form of feedthrough due to astigmatism oriented 45 deg to the track direction. Feedthrough due to other primary aberrations is not improved, but performance is identical to the unimproved astigmatic method.

Highly precise and compact ultrahigh vacuum rotary feedthrough.

PubMed

Aiura, Y; Kitano, K

2012-03-01

The precision and rigidity of compact ultrahigh vacuum (UHV) rotary feedthroughs were substantially improved by preparing and installing an optimal crossed roller bearing with mounting holes. Since there are mounting holes on both the outer and inner races, the bearing can be mounted directly to rotary and stationary stages without any fixing plates and housing. As a result, it is possible to increase the thickness of the bearing or the size of the rolling elements in the bearing without increasing the distance between the rotating and fixing International Conflat flanges of the UHV rotary feedthrough. Larger rolling elements enhance the rigidity of the UHV rotary feedthrough. Moreover, owing to the structure having integrated inner and outer races and mounting holes, the performance is almost entirely unaffected by the installation of the bearing, allowing for a precise optical encoder to be installed in the compact UHV rotary feedthrough. Using position feedback via a worm gear system driven by a stepper motor and a precise rotary encoder, the actual angle of the compact UHV rotary feedthrough can be controlled with extremely high precision.

Highly precise and compact ultrahigh vacuum rotary feedthrough

NASA Astrophysics Data System (ADS)

Aiura, Y.; Kitano, K.

2012-03-01

The precision and rigidity of compact ultrahigh vacuum (UHV) rotary feedthroughs were substantially improved by preparing and installing an optimal crossed roller bearing with mounting holes. Since there are mounting holes on both the outer and inner races, the bearing can be mounted directly to rotary and stationary stages without any fixing plates and housing. As a result, it is possible to increase the thickness of the bearing or the size of the rolling elements in the bearing without increasing the distance between the rotating and fixing International Conflat flanges of the UHV rotary feedthrough. Larger rolling elements enhance the rigidity of the UHV rotary feedthrough. Moreover, owing to the structure having integrated inner and outer races and mounting holes, the performance is almost entirely unaffected by the installation of the bearing, allowing for a precise optical encoder to be installed in the compact UHV rotary feedthrough. Using position feedback via a worm gear system driven by a stepper motor and a precise rotary encoder, the actual angle of the compact UHV rotary feedthrough can be controlled with extremely high precision.

A novel in situ synthesis of SiBCN-Zr composites prepared by a sol-gel process and spark plasma sintering.

PubMed

Miao, Yang; Yang, Zhihua; Liang, Bin; Li, Quan; Chen, Qingqing; Jia, Dechang; Cheng, Yi-Bing; Zhou, Yu

2016-08-09

In the work reported here, SiBCN amorphous powders were first prepared by a mechanical alloying technique, employing cubic silicon, graphite and hexagonal boron nitride powders as raw materials. Zirconia was then introduced via sol-gel methods. The resulting powder composite was then consolidated via SPS sintering. The SPS sintering sample was evaluated using XRD, SEM and TEM. XRD reveals a chemical transformation wherein amorphous BN(C) and ZrO2 form the primary ZrC and ZrB2 phases after SPS processing along with SiC and BN(C). Thereafter ZrC reacts with BN(C) completely to form ZrB2. The reaction starts at the temperature of 1500 °C and is complete at the temperature of 1900 °C. The fracture toughness of the sintered composites reaches 4.9 ± 0.2 MPa m(1/2) due to the presence of the laminated structure of the BN(C) phase.

Glycosylation and immunocytochemistry of binucleate cells in pronghorn (Antilocapra americana, Antilocapridae) show features of both Giraffidae and Bovidae.

PubMed

Jones, Carolyn J P; Silvia, W J; Hamilton, C H; Geary, T W; Zezeski, A L; Wooding, F B P

2017-09-01

Although the pronghorn (Antilocapra americana) resembles an antelope, its nearest relatives are the giraffe and okapi. In this study we have examined the placentae of 6 pronghorns using lectin- and immunocytochemistry to identify giraffid and bovid features. Binucleate cells (BNC) of the placenta exhibited features intermediate between those of the giraffe and bovine; Dolichos biflorus agglutinin binding - strong in the bovine BNC and absent in the giraffe - was evident in only a subpopulation of BNC while binding to blood vessels, as in the giraffe. Binding of Phytolacca americana agglutinin resembled that of the giraffe and okapi whereas many other glycans were found in all four clades. PAG antigens were similar to bovine and okapi but not giraffe. In summary, although the pronghorn outwardly resembles an antelope, placental BNC show giraffid features. Although each clade has its own individual characteristics, there are far more similarities than differences between them, emphasizing the common ancestry of all four clades. Copyright © 2017 Elsevier Ltd. All rights reserved.

Molecular study of wound healing after using biosynthesized BNC/Fe3O4 nanocomposites assisted with a bioinformatics approach

PubMed Central

Moniri, Mona; Boroumand Moghaddam, Amin; Azizi, Susan; Abdul Rahim, Raha; Zuhainis Saad, Wan; Navaderi, Mohammad; Arulselvan, Palanisamy; Mohamad, Rosfarizan

2018-01-01

Background Molecular investigation of wound healing has allowed better understanding about interaction of genes and pathways involved in healing progression. Objectives The aim of this study was to prepare magnetic/bacterial nanocellulose (Fe3O4/BNC) nanocomposite films as ecofriendly wound dressing in order to evaluate their physical, cytotoxicity and antimicrobial properties. The molecular study was carried out to evaluate expression of genes involved in healing of wounds after treatment with BNC/Fe3O4 films. Study design, materials, and methods Magnetic nanoparticles were biosynthesized by using Aloe vera extract in new isolated bacterial nanocellulose (BNC) RM1. The nanocomposites were characterized using X-ray diffraction, Fourier transform infrared, and field emission scanning electron microscopy. Moreover, swelling property and metal ions release profile of the nanocomposites were investigated. The ability of nanocomposites to promote wound healing of human dermal fibroblast cells in vitro was examined. Bioinformatics databases were used to identify genes with important healing effect. Key genes which interfered with healing were studied by quantitative real time PCR. Results Spherical magnetic nanoparticles (15–30 nm) were formed and immobilized within the structure of BNC. The BNC/Fe3O4 was nontoxic (IC50>500 μg/mL) with excellent wound healing efficiency after 48 hours. The nanocomposites showed good antibacterial activity ranging from 6±0.2 to 13.40±0.10 mm against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa. The effective genes for the wound healing process were TGF-B1, MMP2, MMP9, Wnt4, CTNNB1, hsa-miR-29b, and hsa-miR-29c with time dependent manner. BNC/Fe3O4 has an effect on microRNA by reducing its expression and therefore causing an increase in the gene expression of other genes, which consequently resulted in wound healing. Conclusion This eco-friendly nanocomposite with excellent healing properties can be used as an effective wound dressing for treatment of cutaneous wounds. PMID:29861630

Biased normalized cuts for target detection in hyperspectral imagery

NASA Astrophysics Data System (ADS)

Zhang, Xuewen; Dorado-Munoz, Leidy P.; Messinger, David W.; Cahill, Nathan D.

2016-05-01

The Biased Normalized Cuts (BNC) algorithm is a useful technique for detecting targets or objects in RGB imagery. In this paper, we propose modifying BNC for the purpose of target detection in hyperspectral imagery. As opposed to other target detection algorithms that typically encode target information prior to dimensionality reduction, our proposed algorithm encodes target information after dimensionality reduction, enabling a user to detect different targets in interactive mode. To assess the proposed BNC algorithm, we utilize hyperspectral imagery (HSI) from the SHARE 2012 data campaign, and we explore the relationship between the number and the position of expert-provided target labels and the precision/recall of the remaining targets in the scene.

Concentration determination of collagen and proteoglycan in bovine nasal cartilage by Fourier transform infrared imaging and PLS

NASA Astrophysics Data System (ADS)

Zhang, Xuexi; Xiao, Zhi-Yan; Yin, Jianhua; Xia, Yang

2014-09-01

Fourier transform infrared imaging (FTIRI) combined with chemometrics can be used to detect the structure of bio-macromolecule, measure the concentrations of some components, and so on. In this study, FTIRI with Partial Least-Squares (PLS) regression was applied to study the concentration of two main components in bovine nasal cartilage (BNC), collagen and proteoglycan. An infrared spectrum library was built by mixing the collagen and chondroitin 6-sulfate (main of proteoglycan) at different ratios. Some pretreatments are needed for building PLS model. FTIR images were collected from BNC sections at 6.25μm and 25μm pixel size. The spectra extracted from BNC-FTIR images were imported into the PLS regression program to predict the concentrations of collagen and proteoglycan. These PLS-determined concentrations are agreed with the result in our previous work and biochemical analytical results. The prediction shows that the concentrations of collagen and proteoglycan in BNC are comparative on the whole. However, the concentration of proteoglycan is a litter higher than that of collagen, to some extent.

Hermetically sealed electrical feedthrough for high temperature secondary cells

DOEpatents

Knoedler, R.; Nelson, P.A.; Shimotake, H.; Battles, J.E.

1983-07-26

A passthrough seal is disclosed for electrically isolating the terminal in a lithium/metal sulfide cell from the structural cell housing. The seal has spaced upper and lower insulator rings fitted snuggly between the terminal and an annularly disposed upstanding wall, and outwardly of a powdered insulator also confined between the upstanding wall and terminal. The adjacent surfaces of the upper insulator ring and the respective upstanding wall and terminal are conically tapered, diverging in the axial direction away from the cell interior, and a sealing ring is located between each pair of the adjacent surfaces. The components are sized so that upon appropriate movement of the upper insulator ring toward the lower insulator ring the powdered insulator and sealing rings are each compressed to a high degree. This compacts the powdered insulator thereby rendering the same highly impervious and moreover fuses the sealing rings to and between the adjacent surfaces. The upper and lower insulator rings might be formed of beryllium oxide and/or alumina, the powdered insulator might be formed of boron nitride, and the sealing rings might be formed of aluminum.

Hermetically sealed electrical feedthrough for high temperature secondary cells

DOEpatents

Knoedler, Reinhard; Nelson, Paul A.; Shimotake, Hiroshi; Battles, James E.

1985-01-01

A passthrough seal is disclosed for electrically isolating the terminal in a lithium/metal sulfide cell from the structural cell housing. The seal has spaced upper and lower insulator rings fitted snuggly between the terminal and an annularly disposed upstanding wall, and outwardly of a powdered insulator also confined between the upstanding wall and terminal. The adjacent surfaces of the upper insulator ring and the respective upstanding wall and terminal are conically tapered, diverging in the axial direction away from the cell interior, and a sealing ring is located between each pair of the adjacent surfaces. The components are sized so that upon appropriate movement of the upper insulator ring toward the lower insulator ring the powdered insulator and sealing rings are each compressed to a high degree. This compacts the powdered insulator thereby rendering the same highly impervious and moreover fuses the sealing rings to and between the adjacent surfaces. The upper and lower insulator rings might be formed of beryllium oxide and/or alumina, the powdered insulator might be formed of boron nitride, and the sealing rings might be formed of aluminum.

3D culturing and differentiation of SH-SY5Y neuroblastoma cells on bacterial nanocellulose scaffolds.

PubMed

Innala, Marcus; Riebe, Ilse; Kuzmenko, Volodymyr; Sundberg, Johan; Gatenholm, Paul; Hanse, Eric; Johannesson, Sara

2014-10-01

A new in vitro model, mimicking the complexity of nerve tissue, was developed based on a bacterial nanocellulose (BNC) scaffold that supports 3D culturing of neuronal cells. BNC is extracellularly excreted by Gluconacetobacter xylinus (G. xylinus) in the shape of long non-aggregated nanofibrils. The cellulose network created by G. xylinus has good mechanical properties, 99% water content, and the ability to be shaped into 3D structures by culturing in different molds. Surface modification with trimethyl ammonium beta-hydroxypropyl (TMAHP) to induce a positive surface charge, followed by collagen I coating, has been used to improve cell adhesion, growth, and differentiation on the scaffold. In the present study, we used SH-SY5Y neuroblastoma cells as a neuronal model. These cells attached and proliferated well on the BNC scaffold, as demonstrated by scanning electron microscopy (SEM) and the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay. Following neuronal differentiation, we demonstrated functional action potentials (APs) by electrophysiological recordings, indicating the presence of mature neurons on the scaffolds. In conclusion, we have demonstrated for the first time that neurons can attach, proliferate, and differentiate on BNC. This 3D model based on BNC scaffolds could possibly be used for developing in vitro disease models, when combined with human induced pluripotent stem (iPS) cells (derived from diseased patients) for detailed investigations of neurodegenerative disease mechanisms and in the search for new therapeutics.

Tuning the electronic and optical properties of hexagonal boron-nitride nanosheet by inserting graphene quantum dots

NASA Astrophysics Data System (ADS)

Ding, Yi-Min; Shi, Jun-Jie; Zhang, Min; Wu, Meng; Wang, Hui; Cen, Yu-Lang; Pan, Shu-Hang; Guo, Wen-Hui

2018-02-01

It is difficult to integrate two-dimensional (2D) graphene and hexagonal boron-nitride (h-BN) in optoelectronic nanodevices, due to the semi-metal and insulator characteristic of graphene and h-BN, respectively. Using the state-of-the-art first-principles calculations based on many-body perturbation theory, we investigate the electronic and optical properties of h-BN nanosheet embedded with graphene dots. We find that C atom impurities doped in h-BN nanosheet tend to phase-separate into graphene quantum dots (QD), and BNC hybrid structure, i.e. a graphene dot within a h-BN background, can be formed. The band gaps of BNC hybrid structures have an inverse relationship with the size of graphene dot. The calculated optical band gaps for BNC structures vary from 4.71 eV to 3.77 eV, which are much smaller than that of h-BN nanosheet. Furthermore, the valence band maximum is located in C atoms bonded to B atoms and conduction band minimum is located in C atoms bonded to N atoms, which means the electron and hole wave functions are closely distributed around the graphene dot. The bound excitons, localized around the graphene dot, determine the optical spectra of the BNC hybrid structures, in which the exciton binding energies decrease with increase in the size of graphene dots. Our results provide an important theoretical basis for the design and development of BNC-based optoelectronic nanodevices.

Battery cell feedthrough apparatus

DOEpatents

Kaun, Thomas D.

1995-01-01

A compact, hermetic feedthrough apparatus comprising interfitting sleeve portions constructed of chemically-stable materials to permit unique battery designs and increase battery life and performance.

First test of a high voltage feedthrough for liquid Argon TPCs connected to a 300 kV power supply

NASA Astrophysics Data System (ADS)

Cantini, C.; Gendotti, A.; Molina Bueno, L.; Murphy, S.; Radics, B.; Regenfus, C.; Rigaut, Y.-A.; Rubbia, A.; Sergiampietri, F.; Viant, T.; Wu, S.

2017-03-01

Voltages above a hundred kilo-volt will be required to generate the drift field of future very large liquid Argon Time Projection Chambers. One of the most delicate component is the feedthrough whose role is to safely deliver the very high voltage to the cathode through the thick insulating walls of the cryostat without compromising the purity of the argon inside. This requires a feedthrough that is typically meters long and carefully designed to be vacuum tight and have small heat input. Furthermore, all materials should be carefully chosen to allow operation in cryogenic conditions. In addition, electric fields in liquid argon should be kept below a threshold to reduce risks of discharges. The combination of all above requirements represents significant challenges from the design and manufacturing perspective. In this paper, we report on the successful operation of a feedthrough satisfying all the above requirements. The details of the feedthrough design and its manufacturing steps are provided. Very high voltages up to unprecedented voltages of -300 kV could be applied during long periods repeatedly. A source of instability was observed, which was specific to the setup configuration which was used for the test and not due to the feedthrough itself.

Reliability and Characterization of High Voltage Power Capacitors

DTIC Science & Technology

2014-03-01

Cable The HVPS cable is a specialized coaxial cable that utilizes a high voltage bayonet connector. The cable itself has a voltage rating in excess...the( LabVIEW(program( GPIB( CABLE ( HVPS( HVPS( COAXIAL ( CABLE ( BNC( COAXIAL ( CABLE ( BNC( COAXIAL ( CABLE ( CAPACITOR(‘C’(DATA( CAPACITOR(‘A’(DATA( Circuit...16   F.   CABLES AND CONNECTORS ...................................................................16

Method of fabricating high-density hermetic electrical feedthroughs

DOEpatents

Shah, Kedar G.; Pannu, Satinderpall S.; Delima, Terri L.

2015-06-02

A method of fabricating electrical feedthroughs selectively removes substrate material from a first side of an electrically conductive substrate (e.g. a bio-compatible metal) to form an array of electrically conductive posts in a substrate cavity. An electrically insulating material (e.g. a bio-compatible sealing glass) is then flowed to fill the substrate cavity and surround each post, and solidified. The solidified insulating material is then exposed from an opposite second side of the substrate so that each post is electrically isolated from each other as well as the bulk substrate. In this manner a hermetic electrically conductive feedthrough construction is formed having an array of electrical feedthroughs extending between the first and second sides of the substrate from which it was formed.

Battery cell feedthrough apparatus

DOEpatents

Kaun, T.D.

1995-03-14

A compact, hermetic feedthrough apparatus is described comprising interfitting sleeve portions constructed of chemically-stable materials to permit unique battery designs and increase battery life and performance. 8 figs.

The new version 2.12 of BKG Ntrip Client (BNC)

NASA Astrophysics Data System (ADS)

Stürze, Andrea; Mervart, Leos; Weber, Georg; Rülke, Axel; Wiesensarter, Erwin; Neumaier, Peter

2016-04-01

A new version of the BKG Ntrip Client (BNC) has been released. Originally developed in cooperation of the Federal Agency for Cartography and Geodesy (BKG) and the Czech Technical University (CTU) with a focus on multi-stream real-time access to GPS observations, the software has once again been substantially extended. Promoting Open Standards as recommended by the Radio Technical Commission for Maritime Services (RTCM) remains the prime subject. Beside its Graphical User Interface (GUI), the real-time software for Windows, Linux, Mac, and Linux platforms now comes with complete Command Line Interface (CLI) and considerable post processing functionality. RINEX Version 3 file editing & Quality Check (QC) with full support of Galileo, BeiDou, and SBAS - besides GPS and GLONASS - is part of the new features. Comparison of satellite orbit/clock files in SP3 format is another fresh ability of BNC. Simultaneous multi-station Precise Point Positioning (PPP) for real-time displacement-monitoring of entire reference station networks is one more recent addition to BNC. Implemented RTCM messages for PPP (under development) comprise satellite orbit and clock corrections, code and phase observation biases, and the Vertical Total Electron Content (VTEC) of the ionosphere. The well established, mature codebase is mostly written in C++ language. Its publication under GNU GPL is thought to be well-suited for test, validation and demonstration of new approaches in precise real-time satellite navigation when IP streaming is involved. The poster highlights BNC features which are new in version 2.12 and beneficial to IAG institutions and services such as IGS/RT-IGS and to the interested public in general.

Sealed fiber-optic bundle feedthrough

DOEpatents

Tanner, Carol E.

2002-01-01

A sealed fiber-optic bundle feedthrough by which a multitude of fiber-optic elements may be passed through an opening or port in a wall or structure separating two environments at different pressures or temperatures while maintaining the desired pressure or temperature in each environment. The feedthrough comprises a rigid sleeve of suitable material, a bundle of individual optical fibers, and a resin-based sealing material that bonds the individual optical fibers to each other and to the rigid sleeve.

Sodium heat engine electrical feedthrough

DOEpatents

Weber, N.

1985-03-19

A thermoelectric generator device which converts heat energy to electrical energy is disclosed. An alkali metal is used with a solid electrolyte and a hermetically sealed feedthrough structure. 4 figs.

Mutational analysis of hepatitis B virus pre-S1 (9–24) fusogenic peptide

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Qiushi; Somiya, Masaharu; Shimada, Naohiko

A hollow nanoparticle known as a bio-nanocapsule (BNC) consisting of hepatitis B virus (HBV) envelope L protein and liposome (LP) can encapsulate drugs and genes and thereby deliver them in vitro and in vivo to human hepatic tissues, specifically by utilizing the HBV-derived infection machinery. Recently, we identified a low pH-dependent fusogenic domain at the N-terminal part of the pre-S1 region of the HBV L protein (amino acid residues 9 to 24; NPLGFFPDHQLDPAFG), which shows membrane destabilizing activity (i.e., membrane fusion, membrane disruption, and payload release) upon interaction with target LPs. In this study, instead of BNC and HBV, we generated LPsmore » displaying a mutated form of the pre-S1 (9–24) peptide, and performed a membrane disruption assay using target LPs containing pyranine (fluorophore) and p-xylene-bis (N-pyridinium bromide) (DPX) as a quencher. The membrane disruption activity was found to correlate with the hydrophobicity of the whole structure, while the peptide retained a random-coil structure even under low pH condition. One large hydrophobic cluster (I) and one small hydrophobic cluster (II) residing in the peptide would be connected by the protonation of residues D16 and D20, and thereby exhibit strong membrane disruption activity in a low pH-dependent manner. Furthermore, the introduction of a positively charged residue enhanced the activity significantly, suggesting that a sole positively charged residue (H17) may be important for the interaction with target LPs by electrostatic interaction. Collectively, these results suggest that the pre-S1 (9–24) peptide may be involved in the endosomal escape of the BNC's payloads, as well as in the HBV uncoating process. -- Highlights: •Low pH-dependent fusogenic domain of hepatitis B virus pre-S1 region is analyzed. •The domain resides in pre-S1 (9–24) region, exhibiting random-coil structure. •Membrane disruption activity of the domain is mainly driven by its hydrophobicity. •Two Asp residues of the domain function as low-pH sensing molecule.« less

Control of vacuum induction brazing system for sealing of instrumentation feed-through

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sung Ho Ahn; Jintae Hong; Chang Young Joung

2015-07-01

The integrity of instrumentation cables is an important performance parameter in addition to the sealing performance in the brazing process. An accurate brazing control was developed for the brazing of the instrumentation feed-through in the vacuum induction brazing system in this paper. The experimental results show that the accurate brazing temperature control performance is achieved by the developed control scheme. Consequently, the sealing performances of the instrumentation feed-through and the integrities of the instrumentation cables were satisfied after brazing. (authors)

Control of Vacuum Induction Brazing System for Sealing of Instrumentation Feedthrough

NASA Astrophysics Data System (ADS)

Ahn, Sung Ho; Hong, Jintae; Joung, Chang Young; Heo, Sung Ho

2017-04-01

The integrity of instrumentation cables is an important performance parameter in the brazing process, along with the sealing performance. In this paper, an accurate control scheme for brazing of the instrumentation feedthrough in a vacuum induction brazing system was developed. The experimental results show that the accurate brazing temperature control performance is achieved by the developed control scheme. It is demonstrated that the sealing performances of the instrumentation feedthrough and the integrity of the instrumentation cables are to be acceptable after brazing.

Method of fabricating high-density hermetic electrical feedthroughs using insulated wire bundles

DOEpatents

Shah, Kedar G.; Benett, William J.; Pannu, Satinderpall S.

2016-05-10

A method of fabricating electrical feedthroughs coats of a plurality of electrically conductive wires with an electrically insulating material and bundles the coated wires together in a substantially parallel arrangement. The bundled coated wires are secured to each other by joining the electrically insulating material of adjacent wires together to form a monolithic block which is then cut transverse to the wires to produce a block section having opposing first and second sides with a plurality of electrically conductive feedthroughs extending between them.

Investigation of thermally activated deformation in amorphous PMMA and Zr-Cu-Al bulk metallic glasses with broadband nanoindentation creep

Treesearch

J.B. Puthoff; J.E. Jakes; H. Cao; D.S. Stone

2009-01-01

The development of nanoindentation test systems with high data collection speeds has made possible a novel type of indentation creep test: broadband nanoindentation creep (BNC). Using the high density of data points generated and analysis techniques that can model the instantaneous projected indent area at all times during a constant-load indentation experiment, BNC...

Ultra-high-vacuum electrical feedthrough

NASA Technical Reports Server (NTRS)

Gavaler, J. R.; Janocko, M. A.

1976-01-01

Device for cathodic sputtering utilizes cathode dark-space region adjacent to high negative-potential surfaces. Feedthrough is made of metal and glass, is helium leaktight, and is bakeable; it can be incorporated into any vacuum apparatus.

A multiplexable, microfluidic platform for the rapid quantitation of a biomarker panel for early ovarian cancer detection at the point-of-care

PubMed Central

Shadfan, Basil H.; Simmons, Archana R.; Simmons, Glennon W.; Ho, Andy; Wong, Jorge; Lu, Karen H.; Bast, Robert C.; McDevitt, John T.

2015-01-01

Point-of-care (POC) diagnostic platforms have the potential to enable low-cost, large-scale screening. As no single biomarker is shed by all ovarian cancers, multiplexed biomarker panels promise improved sensitivity and specificity to address the unmet need for early detection of ovarian cancer. We have configured the programmable bio-nano-chip (p-BNC) - a multiplexable, microfluidic, modular platform - to quantify a novel multimarker panel comprising CA125, HE4, MMP-7 and CA72-4. The p-BNC is a bead-based immunoanalyzer system with a credit-card-sized footprint that integrates automated sample metering, bubble and debris removal, reagent storage and waste disposal, permitting POC analysis. Multiplexed p-BNC immunoassays demonstrated high specificity, low cross-reactivity, low limits of detection suitable for early detection, and a short analysis time of 43 minutes. Day-to-day variability, a critical factor for longitudinally monitoring biomarkers, ranged between 5.4–10.5%, well below the biological variation for all four markers. Biomarker concentrations for 31 late-stage sera correlated well (R2 = 0.71 to 0.93 for various biomarkers) with values obtained on the Luminex® platform. In a 31 patient cohort encompassing early- and late-stage ovarian cancers along with benign and healthy controls, the multiplexed p-BNC panel was able to distinguish cases from controls with 68.7% sensitivity at 80% specificity. Utility for longitudinal biomarker monitoring was demonstrated with pre-diagnostic sera from 2 cases and 4 controls. Taken together, the p-BNC shows strong promise as a diagnostic tool for large-scale screening that takes advantage of faster results and lower costs while leveraging possible improvement in sensitivity and specificity from biomarker panels. PMID:25388014

Customized Hermetic Feedthrough Developed to Isolate Fluids

NASA Technical Reports Server (NTRS)

Meredith, Roger D.

1999-01-01

A common problem occurs when refrigerant fluids wick inside the insulation of thermocouple wires through a compressor's casing feedthrough and then leak into the adjacent disconnect box outside the casing. Leaking fluids create an unfavorable situation inside the disconnect box and may contaminate the fluids. To address this problem, NASA Lewis Research Center s Manufacturing Engineering Division developed a customized hermetic feedthrough for a bank of Worthington compressors. In these compressors, bearing temperatures are measured by internal thermocouples embedded in bearings located inside the compressor casings. The thermocouple wires need to be routed outside the casing and read at another location. These wires are short and are terminated to a disconnect strip inside the casing. The bearings operate at about 170 F, but because the casing is filled with R12 refrigerant oil, the casing has a maximum temperature of about 100 F. The operating conditions of these compressors permit the use of an epoxy that is compatible with the R12 fluid. The desired finished product is a stainless steel tube that has been filled solid with epoxy after thermocouple wires bonded and sealed by epoxy have been inserted through its length. Shrink tubing extends from both ends of the tube. The process that was developed to isolate the thermocouple wires from the R12 fluid follows. For this application, use an 8-in.-long piece of 0.500-in. 304 stainless steel tube with six pairs of 24-gauge stranded, PTFE-insulated (polytetrafluoroethylene) type "T" thermocouple wires for each feedthrough. Use shrink tubing to strain relief the insulated wires at their exit from the stainless steel tube. Cut the wire to length and identify the location of the stainless steel tube sleeve with masking tape. Then, remove the outer insulation from a 2-in. section of wire that will be inside the tube, and carefully strip to bare wire a 1-in. section in the middle of the section with the outer insulation removed. For an effective seal, the epoxy must penetrate between the strands when stranded conductors are used. Make the seal with epoxy bond on the bare wire. The bare wire must be encapsulated with a thin layer of the epoxy that leaves only a very low profile. These encapsulated wires must cure before the assembly can be continued. Then, inspect the cured wires for complete encapsulation before going to the next step. Insert the wires in the stainless steel tube and orient them so that the epoxied stripped sections are staggered within the tube; then, apply shrink tubing to one end of the cleaned wires, positioning it inside the edge of the tube. The small gaps between the wires on the other end will be used to inject the epoxy into the tube. Let the epoxy cure inside the tube, free of any voids. Then, continue to fill the tube until the entire 8-in. length is nearly filled, allowing room for the other strain-relieving shrink tubing. Since this first design, the process has been adjusted to fit many needs and situations. Customized feedthroughs have been assembled from various wire types, wire gauges, and/or stainless steel tube passages. The fittings selected to mount these feedthroughs allow their use in other areas, such as pressure or vacuum systems.

Shear transformation zone activation during deformation in bulk metallic glasses characterized using a new indentation creep technique

Treesearch

J.B. Puthoff; H.B. Cao; Joseph E. Jakes; P.M. Voyles; D.S. Stone

2009-01-01

We have developed a novel type of nanoindentation creep experiment, called broadband nanoindentation creep (BNC), and used it to characterize the thermal activation of shear transformation zones (STZs) in three BMGs in the Zr-Cu-Al system. Using BNC, material hardness can be determined across a wide range of strain rates (10–4 to 10 s–...

A Novel Small Molecule Modulator of Amyloid Pathology.

PubMed

Lovell, Mark A; Lynn, Bert C; Fister, Shuling; Bradley-Whitman, Melissa; Murphy, M Paul; Beckett, Tina L; Norris, Christopher M

2016-05-04

Because traditional approaches to drug development for Alzheimer's disease are becoming increasingly expensive and in many cases disappointingly unsuccessful, alternative approaches are required to shift the paradigm. Following leads from investigations of dihydropyridine calcium channel blockers, we observed unique properties from a class of functionalized naphthyridines and sought to develop these as novel therapeutics that minimize amyloid pathology without the adverse effects associated with current therapeutics. Our data show methyl 2,4-dimethyl-5-oxo-5,6-dihydrobenzo[c][2,7]naphthyridine-1-carboxylate (BNC-1) significantly decreases amyloid burden in a well-established mouse model of amyloid pathology through a unique mechanism mediated by Elk-1, a transcriptional repressor of presenilin-1. Additionally, BNC-1 treatment leads to increased levels of synaptophysin and synapsin, markers of synaptic integrity, but does not adversely impact presenilin-2 or processing of Notch-1, thus avoiding negative off target effects associated with pan-gamma secretase inhibition. Overall, our data show BNC-1 significantly decreases amyloid burden and improves markers of synaptic integrity in a well-established mouse model of amyloid deposition by promoting phosphorylation and activation of Elk-1, a transcriptional repressor of presenilin-1 but not presenilin-2. These data suggest BNC-1 might be a novel, disease-modifying therapeutic that will alter the pathogenesis of Alzheimer's disease.

Trinucleate uterine epithelial cells as evidence for White-tail Deer trophoblast binucleate cell migration and as markers of placental binucleate cell dynamics in a variety of wild ruminants.

PubMed

Wooding, F B Peter; Osborn, David; Killian, Gary J

2018-02-01

The unicellular trophoblast epithelium of all ruminants so far investigated contains 15-20% binucleate cells with numerous secretory granules. Electron microscope (EM) studies of the domesticated cow, ewe, goat and deer species have established that these BNC migrate out of the trophoblast epithelium to fuse with the apposed maternal uterine epithelial cells or derivative to form fetomaternal tissue throughout pregnancy. However there is one careful EM study of the trophoblast of a wild ruminant, the White-tail deer, which found the usual number of BNC but no evidence of any migration or fusion. Since there are up to 200 species of wild ruminants, it was important to establish whether there really are two possible scenarios for BNC function. This paper reports a light microscope (LM) immunocytochemical study of cell dynamics in ruminant placentas using 1-2 mμ deresinated sections. The results clearly demonstrate that the White-tail deer and all of the other 15 (see Table 1) randomly selected wild ruminants show the same BNC migration and fusion pattern. These results suggest that this remarkable cellular behaviour is fundamental to the ruminant evolutionary success. Copyright © 2017 Elsevier Ltd. All rights reserved.

Waveform synthesis for imaging and ranging applications

DOEpatents

Doerry, Armin W.; Dudley, Peter A.; Dubert, Dale F.; Tise, Bertice L.

2004-12-07

Frequency dependent corrections are provided for quadrature imbalance and Local Oscillator (LO) feed-through. An operational procedure filters imbalance and LO feed-through effects without prior calibration or equalization. Waveform generation can be adjusted/corrected in a synthetic aperture radar system (SAR), where a rolling phase shift is applied to the SAR's QDWS signal where it is demodulated in a receiver; unwanted energies, such as LO feed-through and/or imbalance energy, are separated from a desired signal in Doppler; the separated energy is filtered from the receiver leaving the desired signal; and the separated energy in the receiver is measured to determine the degree of imbalance that is represented by it. Calibration methods can also be implemented into synthesis. The degree of LO feed-through and imbalance can be used to determine calibration values that can then be provided as compensation for frequency dependent errors in components, such as the QDWS and SSB mixer, affecting quadrature signal quality.

Waveform Synthesizer For Imaging And Ranging Applications

DOEpatents

Dubbert, Dale F.; Dudley, Peter A.; Doerry, Armin W.; Tise, Bertice L.

2004-12-28

Frequency dependent corrections are provided for Local Oscillator (LO) feed-through. An operational procedure filters LO feed-through effects without prior calibration or equalization. Waveform generation can be adjusted/corrected in a synthetic aperture radar system (SAR), where a rolling phase shift is applied to the SAR's QDWS signal where it is demodulated in a receiver, unwanted energies, such as LO feed-through energy, are separated from a desired signal in Doppler; the separated energy is filtered from the receiver leaving the desired signal; and the separated energy in the receiver is measured to determine the degree of imbalance that is represented by it. Calibration methods can also be implemented into synthesis. The degree of LO feed-through can be used to determine calibration values that can then be provided as compensation for frequency dependent errors in components, such as the QDWS and SSB mixer, affecting quadrature signal quality.

Development of a high power electron beam welding gun with replaceable high voltage feed-through insulators

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saha, T.K; Mascarenhas, M.; Kandaswamy, E., E-mail: tanmay@barc.gov.in

Ceramic to metal sealed feed-through insulators are commonly used in electron beam welding gun. The above feed-through insulators are susceptible to failure, as the brazing joints in them are not always very strong. Failure in one of these feed-through could render the complete gun unusable. This problem has already been faced in BARC, which led to the development of the electron gun with replaceable feed through insulators. A 24 kW Electron Beam Welding (EBW) gun with indigenous designed replaceable insulators is fabricated in BARC. Emphasis during the design of the gun had been to reduce the use of imported componentsmore » to zero. This paper describes the design and fabrication of this gun and reports various simulations and tests performed. Beam trajectory of the gun is numerically computed and presented. Weld passes were carried out on stainless steel plates show satisfactory penetrations. (author)« less

Basic newborn care and neonatal resuscitation: a multi-country analysis of health system bottlenecks and potential solutions

PubMed Central

2015-01-01

Background An estimated two-thirds of the world's 2.7 million newborn deaths could be prevented with quality care at birth and during the postnatal period. Basic Newborn Care (BNC) is part of the solution and includes hygienic birth and newborn care practices including cord care, thermal care, and early and exclusive breastfeeding. Timely provision of resuscitation if needed is also critical to newborn survival. This paper describes health system barriers to BNC and neonatal resuscitation and proposes solutions to scale up evidence-based strategies. Methods The maternal and newborn bottleneck analysis tool was applied by 12 countries in Africa and Asia as part of the Every Newborn Action Plan process. Country workshops engaged technical experts to complete the survey tool, which is designed to synthesise and grade health system "bottlenecks" that hinder the scale up of maternal-newborn intervention packages. We used quantitative and qualitative methods to analyse the bottleneck data, combined with literature review, to present priority bottlenecks and actions relevant to different health system building blocks for BNC and neonatal resuscitation. Results Eleven of the 12 countries provided grading data. Overall, bottlenecks were graded more severely for resuscitation. The most severely graded bottlenecks for BNC were health workforce (8 of 11 countries), health financing (9 out of 11) and service delivery (7 out of 9); and for neonatal resuscitation, workforce (9 out of 10), essential commodities (9 out of 10) and service delivery (8 out of 10). Country teams from Africa graded bottlenecks overall more severely. Improving workforce performance, availability of essential commodities, and well-integrated health service delivery were the key solutions proposed. Conclusions BNC was perceived to have the least health system challenges among the seven maternal and newborn intervention packages assessed. Although neonatal resuscitation bottlenecks were graded more severe than for BNC, similarities particularly in the workforce and service delivery building blocks highlight the inextricable link between the two interventions and the need to equip birth attendants with requisite skills and commodities to assess and care for every newborn. Solutions highlighted by country teams include ensuring more investment to improve workforce performance and distribution, especially numbers of skilled birth attendants, incentives for placement in challenging settings, and skills-based training particularly for neonatal resuscitation. PMID:26391000

Early Dexamethasone Treatment Induces Placental Apoptosis in Sheep

PubMed Central

Meng, Wenbin; Shang, Hongkai; Li, Shaofu; Sloboda, Deborah M.; Ehrlich, Loreen; Lange, Karolin; Xu, Huaisheng; Henrich, Wolfgang; Dudenhausen, Joachim W.; Plagemann, Andreas; Newnham, John P.; Challis, John R. G.

2015-01-01

Glucocorticoid treatment given in late pregnancy in sheep resulted in altered placental development and function. An imbalance of placental survival and apoptotic factors resulting in an increased rate of apoptosis may be involved. We have now investigated the effects of dexamethasone (DEX) in early pregnancy on binucleate cells (BNCs), placental apoptosis, and fetal sex as a determinant of these responses. Pregnant ewes carrying singleton fetuses (n = 105) were randomized to control (n = 56, 2 mL saline/ewe) or DEX treatment (n = 49, intramuscular injections of 0.14 mg/kg ewe weight per 12 hours over 48 hours) at 40 to 41 days of gestation (dG). Placentomes were collected at 50, 100, 125, and 140 dG. At 100 dG, DEX in females reduced BNC numbers, placental antiapoptotic (proliferating cell nuclear antigen), and increased proapoptotic factors (Bax, p53), associated with a temporarily decrease in fetal growth. At 125 dG, BNC numbers and apoptotic markers were restored to normal. In males, ovine placental lactogen-protein levels after DEX were increased at 50 dG, but at 100 and 140 dG significantly decreased compared to controls. In contrast to females, these changes were independent of altered BNC numbers or apoptotic markers. Early DEX was associated with sex-specific, transient alterations in BNC numbers, which may contribute to changes in placental and fetal development. Furthermore, in females, altered placental apoptosis markers may be involved. PMID:25063551

Diverse carrier mobility of monolayer BNC_x: A combined density functional theory and Boltzmann transport theory study.

PubMed

Wu, Tao; Deng, Kaiming; Deng, Wei-Qiao; Lu, Ruifeng

2017-09-19

BNCX monolayer as a kind of two-dimensional material has numerous chemical atomic ratios and arrangements with different electronic structures. Via calculations on the basis of density functional theory and Boltzmann transport theory under deformation potential approximation, the band structures and carrier mobilities of BNCX (x=1,2,3,4) nanosheets are systematically investigated. The calculated results show that BNC2-1 is a material with very small band gap (0.02 eV) among all the structures while other BNCX monolayers are semiconductors with band gap ranging from 0.51 to 1.32 eV. The carrier mobility of BNCX varies considerably from tens to millions of cm2 V-1 s-1. For BNC2-1, the hole mobility and electron mobility along both x and y directions can reach 105 orders of magnitude, which is similar to the carrier mobility of graphene. Besides, all studied BNCX monolayers obviously have anisotropic hole mobility and electron mobility. In particular, for semiconductor BNC4, its hole mobility along y direction and electron mobility along x direction unexpectedly reach 106 orders of magnitude, even higher than that of graphene. Our findings suggest that BNCX layered materials with proper ratio and arrangement of carbon atoms will possess desirable charge transport properties, exhibiting potential applications in nanoelectronic devices. © 2017 IOP Publishing Ltd.

Linear motion feed through with thin wall rubber sealing element

NASA Astrophysics Data System (ADS)

Mikhailov, V. P.; Deulin, E. A.

2017-07-01

The patented linear motion feedthrough is based on elastic thin rubber walls usage being reinforced with analeptic string fixed in the middle part of the walls. The pneumatic or hydro actuators create linear movement of stock. The length of this movement is two times more the rubber wall length. This flexible wall is a sealing element of feedthrough. The main advantage of device is negligible resistance force that is less then mentioned one in sealing bellows that leads to positioning error decreasing. Nevertheless, the thin wall rubber sealing element (TRE) of the feedthrough is the main unreliable element that was the reason of this element longevity research. The theory and experimental results help to create equation for TRE longevity calculation under vacuum or extra high pressure difference action. The equation was used for TRE longevity determination for hydraulic or vacuum equipment realization also as it helps for gas flow being leaking through the cracks in thin walls of rubber sealing element of linear motion feedthrough calculation.

Robotic Y-V Plasty for Recalcitrant Bladder Neck Contracture.

PubMed

Granieri, Michael A; Weinberg, Aaron C; Sun, Jeffrey Y; Stifleman, Michael; Zhao, Lee

2018-07-01

To demonstrate the technique and the outcomes of robot assisted Y-V plasty bladder neck reconstruction (RYVBNR). We present our technique for treatment of recalcitrant bladder neck contracture (BNC) in 7 patients who underwent RYVBNR at our institution between March 2016 and September 2017. Indication for the procedure was incomplete emptying, recurrent urinary tract infections, and dysuria. On follow-up, patients were assessed for clinical success by absence of infections, symptoms, and cystoscopic evaluation. Robotic assisted dissection is performed to open the space of Retzius and mobilize the bladder. The cystoscope is passed to the level of the BNC, and Firefly technology is used to localize the BNC. The BNC is incised anteriorly, and a V-shaped bladder flap is advanced into the BNC in a Y-V plasty fashion. We place a perioperative closed suction drain, which is removed before discharge, and a 22 Fr catheter, which that will be removed in the office at approximately 2 weeks. Six men developed recalcitrant BNCs and 1 developed a recalcitrant vesicourethral anastomotic stenosis. All patients had previously undergone an endoscopic procedure. Median time for last attempt at endoscopic management to robot-assisted bladder neck repair was 4.7 months. The average number of prior attempts at endoscopic management was 2. All patients underwent RYVBNR without conversion to open surgery. The median operative time was 240 minutes, estimated blood loss was 67 mL, and length of stay was 1 day. There were no intraoperative complications. Catheters were removed in the office at a median time of 15 days. At a median follow-up of 8 months, all cases were successful with no evidence of recurrence. Only 2 patients had persistent urinary incontinence at 1 pad per day. RYVBNR with a Y-V plasty is a feasible and effective technique for managing a difficult reconstructive problem. Copyright © 2018 Elsevier Inc. All rights reserved.

Feed-through connector couples RF power into vacuum chamber

NASA Technical Reports Server (NTRS)

Grandy, G. L.

1967-01-01

Feed-through device connects RF power to an RF coil in a vacuum chamber. The coil and leads are water cooled and vacuum tight seals are provided at the junctions. The device incorporates silver soldered copper tubes, polytetrafluoroethylene electrical insulators, and O-ring vacuum seals.

Industrialization of the ion plating process

NASA Technical Reports Server (NTRS)

Spalvins, T.

1976-01-01

A new process referred to as ion plating by induction heating (IPIH) is described, which combines the advantages of both ion plating and induction heating. The IPIH apparatus consists of the specimen (cathode) to be coated and the evaporation heating source, which is a ceramic crucible containing the metal to be heated. The specimen is an internal part of the high-voltage ceramic-metal vacuum feedthrough and is connected to the negative terminal of the high-voltage power supply, the positive terminal of the power supply being grounded. The plating conditions are the same as those most commonly used in industrial ion plating. A number of metals - such as nickel, iron, platinum - which were practically impossible to deposit by resistance heating evaporation can now be effectively evaporated and deposited to any desired thickness. Excellent adherence is observed for many metals deposited on various metal surfaces in thicknesses from 0.15 to 50 microns, regardless of the materials selected for coating and substrate.

Biotransformation of sweet lime pulp waste into high-quality nanocellulose with an excellent productivity using Komagataeibacter europaeus SGP37 under static intermittent fed-batch cultivation.

PubMed

Dubey, Swati; Singh, Jyoti; Singh, R P

2018-01-01

Herein, sweet lime pulp waste (SLPW) was utilized as a low- or no-cost feedstock for the production of bacterial nanocellulose (BNC) alone and in amalgamation with other nutritional supplements by the isolate K. europaeus SGP37 under static batch and static intermittent fed-batch cultivation. The highest yield (26.2±1.50gL -1 ) was obtained in the hot water extract of SLPW supplemented with the components of HS medium, which got further boosted to 38±0.85gL -1 as the cultivation strategy was shifted from static batch to static intermittent fed-batch. BNC obtained from various SLPW medium was similar or even superior to that obtained with standard HS medium in terms of its physicochemical properties. The production yields of BNC thus obtained are significantly higher and fit well in terms of industrial scale production. Copyright © 2017 Elsevier Ltd. All rights reserved.

Targeting of polyplex to human hepatic cells by bio-nanocapsules, hepatitis B virus surface antigen L protein particles.

PubMed

Somiya, Masaharu; Yoshimoto, Nobuo; Iijima, Masumi; Niimi, Tomoaki; Dewa, Takehisa; Jung, Joohee; Kuroda, Shun'ichi

2012-06-15

We have previously demonstrated that lipoplex, a complex of cationic liposomes and DNA, could be targeted to human hepatic cells in vitro and in vivo by conjugation with bio-nanocapsules (BNCs) comprising hepatitis B virus (HBV) surface antigen L protein particles. Because the BNC-lipoplex complexes were endowed with the human hepatic cell-specific infection machinery from HBV, the complexes showed excellent specific transfection efficiency in human hepatic cells. In this study, we have found that polyplex (a complex of polyethyleneimine (PEI) and DNA) could form stable complexes with BNCs spontaneously. The diameter and ζ-potential of BNC-polyplex complexes are about 240 nm and +3.54 mV, respectively, which make them more suitable for in vivo use than polyplex alone. BNC-polyplex complexes with an N/P ratio (the molar ratio of the amine group of PEI to the phosphate group of DNA) of 40 showed excellent transfection efficiency in human hepatic cells. When acidification of endosomes was inhibited by bafilomycin A1, the complexes showed higher transfection efficiency than polyplex itself, strongly suggesting that the complexes escaped from endosomes by both fusogenic activity of BNCs and proton sponge activity of polyplex. Furthermore, the cytotoxicity is comparable to that of polyplex of the same N/P value. Thus, BNC-polyplex complexes would be a promising gene delivery carrier for human liver-specific gene therapy. Copyright © 2012 Elsevier Ltd. All rights reserved.

A radio frequency coaxial feedthrough

DOEpatents

Owens, T.L.

1987-12-07

An improved radio frequency coaxial transmission line vacuum feedthrough is provided based on the use of a half-wavelength annular dielectric pressure barrier disk, or multiple disks comprising an effective half wavelength structure to eliminate reflection from the barrier surfaces. Gas-tight seals are formed about the outer and inner diameter surfaces of the barrier disk using a sealing technique which generates radial forces sufficient to form seals by forcing the conductor walls against the surfaces of the barrier disks in a manner which does not deform the radii of the inner and outer conductors, thereby preventing enhancement of the electric field at the barrier faces which limits the voltage and power handling capabilities of a feedthrough.

Radio frequency coaxial feedthrough

DOEpatents

Owens, Thomas L.

1989-01-17

An improved radio frequency coaxial transmission line vacuum feed-through provided based on the use of a half-wavelength annular dielectric pressure barrier disk, or multiple disks comprising an effective half wavelength structure to eliminate reflections from the barrier surfaces. Gas-tight seals are formed about the outer and inner diameter surfaces of the barrier disk using a sealing technique which generates radial forces sufficient to form seals by forcing the conductor walls against the surfaces of the barrier disks in a manner which does not deform the radii of the inner and outer conductors, thereby preventing enhancement of the electric field at the barrier faces which limits voltage and power handling capabilities of a feedthrough.

Cable Feedthrough Between Liquid Oxygen And Ambient

NASA Technical Reports Server (NTRS)

Myers, Don A.

1992-01-01

Encapsulant and back pressure provide double protection. Cable-feedthrough tube between ambient air and interior of vessel containing liquid oxygen protects external instrumentation and cable from oxygen. Cable in tube surrounded by potting compound. Provides flow of gaseous nitrogen to dilute oxidant and makes it harmless in case of leakage through crack in potting compound.

KSC-07pd3652

NASA Image and Video Library

2007-12-21

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a United Space Alliance technician carefully cuts away the foam insulation surrounding the covers over the feed-through connector box on the external tank for space shuttle Atlantis' STS-122 mission. The covers will be removed for access to the feed-through connectors. Following the failure of some of the tank's engine cutoff sensors, or ECO sensors, during propellant tanking for launch attempts on Dec. 6 and Dec. 9, a tanking test was conducted on Dec. 18 to aid in troubleshooting the cause. Technicians spliced test wiring into the ECO sensor electrical system and used time domain reflectometry equipment to help locate the electrical anomaly. Results of the tanking test pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. During the holiday period, workers from Lockheed Martin will begin inspecting and testing the connector. Shuttle program managers will meet on Dec. 27 to review the test and analysis, and decide on a forward plan. Photo credit: NASA/Kim Shiflett

Propulsion and Power Rapid Response Research and Development (R&D) Support. Delivery Order 0011: Analysis of Synthetic Aviation Fuels

DTIC Science & Technology

2011-04-01

from a solvent bottle 4) Allow the k-cell to drain thoroughly 7 Approved for public release; distribution unlimited. 5) Submerge the k-cell into a...beaker filled with isopropanol. Do not submerge the BNC connectors of the k-cell. 6) Remove the k-cell from the isopropanol. 7) Repeat steps 5-6...two more times 8) Allow the k-cell to drain thoroughly. 9) Submerge the k-cell into a second beaker filled with isopropanol. Do not submerge the BNC

High voltage variable diameter insulator

DOEpatents

Vanacek, D.L.; Pike, C.D.

1982-07-13

A high voltage feedthrough assembly having a tubular insulator extending between the ground plane ring and the high voltage ring. The insulator is made of Pyrex and decreases in diameter from the ground plane ring to the high voltage ring, producing equipotential lines almost perpendicular to the wall of the insulator to optimize the voltage-holding capability of the feedthrough assembly.

KSC-08pd0040

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- Mike Berger, with Lockheed Martin, examines the replacement feed-through connector for the engine cutoff, or ECO, sensor system in space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

KSC-08pd0043

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- Lloyd Johns, with Lockheed Martin, attaches the replacement feed-through connector in the engine cutoff, or ECO, sensor system to the internal connector on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

KSC-08pd0044

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- Lloyd Johns, with Lockheed Martin, attaches the replacement feed-through connector in the engine cutoff, or ECO, sensor system to the internal connector on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

KSC-08pd0041

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- This closeup of the replacement feed-through connector for the engine cutoff, or ECO, sensor system on space shuttle Atlantis shows the pins that were soldered at Kennedy. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

Application of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluids.

PubMed

Christodoulides, Nicolaos; De La Garza, Richard; Simmons, Glennon W; McRae, Michael P; Wong, Jorge; Newton, Thomas F; Smith, Regina; Mahoney, James J; Hohenstein, Justin; Gomez, Sobeyda; Floriano, Pierre N; Talavera, Humberto; Sloan, Daniel J; Moody, David E; Andrenyak, David M; Kosten, Thomas R; Haque, Ahmed; McDevitt, John T

2015-08-01

There is currently a gap in on-site drug of abuse monitoring. Current detection methods involve invasive sampling of blood and urine specimens, or collection of oral fluid, followed by qualitative screening tests using immunochromatographic cartridges. While remote laboratories then may provide confirmation and quantitative assessment of a presumptive positive, this instrumentation is expensive and decoupled from the initial sampling making the current drug-screening program inefficient and costly. The authors applied a noninvasive oral fluid sampling approach integrated with the in-development chip-based Programmable bio-nano-chip (p-BNC) platform for the detection of drugs of abuse. The p-BNC assay methodology was applied for the detection of tetrahydrocannabinol, morphine, amphetamine, methamphetamine, cocaine, methadone and benzodiazepines, initially using spiked buffered samples and, ultimately, using oral fluid specimen collected from consented volunteers. Rapid (∼10min), sensitive detection (∼ng/mL) and quantitation of 12 drugs of abuse was demonstrated on the p-BNC platform. Furthermore, the system provided visibility to time-course of select drug and metabolite profiles in oral fluids; for the drug cocaine, three regions of slope were observed that, when combined with concentration measurements from this and prior impairment studies, information about cocaine-induced impairment may be revealed. This chip-based p-BNC detection modality has significant potential to be used in the future by law enforcement officers for roadside drug testing and to serve a variety of other settings, including outpatient and inpatient drug rehabilitation centers, emergency rooms, prisons, schools, and in the workplace. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Application of Programmable Bio-Nano-Chip System for the Quantitative Detection of Drugs of Abuse in Oral Fluids*

PubMed Central

Christodoulides, Nicolaos; De La Garza, Richard; Simmons, Glennon W.; McRae, Michael P.; Wong, Jorge; Newton, Thomas F.; Smith, Regina; Mahoney, James J.; Hohenstein, Justin; Gomez, Sobeyda; Floriano, Pierre N.; Talavera, Humberto; Sloan, Daniel J.; Moody, David E.; Andrenyak, David M.; Kosten, Thomas R.; Haque, Ahmed; McDevitt, John T.

2015-01-01

Objective There is currently a gap in on-site drug of abuse monitoring. Current detection methods involve invasive sampling of blood and urine specimens, or collection of oral fluid, followed by qualitative screening tests using immunochromatographic cartridges. While remote laboratories then may provide confirmation and quantitative assessment of a presumptive positive, this instrumentation is expensive and decoupled from the initial sampling making the current drug-screening program inefficient and costly. The authors applied a noninvasive oral fluid sampling approach integrated with the in-development chip-based Programmable Bio-Nano-Chip (p-BNC) platform for the detection of drugs of abuse. Method The p-BNC assay methodology was applied for the detection of tetrahydrocannabinol, morphine, amphetamine, methamphetamine, cocaine, methadone and benzodiazepines, initially using spiked buffered samples and, ultimately, using oral fluid specimen collected from consented volunteers. Results Rapid (~10 minutes), sensitive detection (~ng/ml) and quantitation of 12 drugs of abuse was demonstrated on the p-BNC platform. Furthermore, the system provided visibility to time-course of select drug and metabolite profiles in oral fluids; for the drug cocaine, three regions of slope were observed that, when combined with concentration measurements from this and prior impairment studies, information about cocaine-induced impairment may be revealed. Conclusions This chip-based p-BNC detection modality has significant potential to be used in the future by law enforcement officers for roadside drug testing and to serve a variety of other settings, including outpatient and inpatient drug rehabilitation centers, emergency rooms, prisons, schools, and in the workplace. PMID:26048639

Programmable bio-nano-chip system: a flexible point-of-care platform for bioscience and clinical measurements

PubMed Central

McRae, Michael. P.; Simmons, Glennon. W.; Wong, Jorge; Shadfan, Basil; Gopalkrishnan, Sanjiv; Christodoulides, Nicolaos

2015-01-01

The development of integrated instrumentation for universal bioassay systems serves as a key goal for the lab-on-a-chip community. The programmable bio-nano-chip (p-BNC) system is a versatile multiplexed and multiclass chemical- and bio-sensing system for bioscience and clinical measurements. The system is comprised of two main components, a disposable cartridge and a portable analyzer. The customizable single-use plastic cartridges, which now can be manufactured in high volumes using injection molding, are designed for analytical performance, ease of use, reproducibility, and low cost. These labcard devices implement high surface area nano-structured biomarker capture elements that enable high performance signaling and are index matched to real-world biological specimens. This detection modality, along with the convenience of on-chip fluid storage in blisters and self-contained waste, represents a standard process to digitize biological signatures at the point-of-care. A companion portable analyzer prototype has been developed to integrate fluid motivation, optical detection, and automated data analysis, and it serves as the human interface for complete assay automation. In this report, we provide a systems-level perspective of the p-BNC universal biosensing platform with an emphasis on flow control, device integration, and automation. To demonstrate the flexibility of the p-BNC, we distinguish diseased and non-case patients across three significant disease applications: prostate cancer, ovarian cancer, and acute myocardial infarction. Progress towards developing a rapid 7 minute myoglobin assay is presented using the fully automated p-BNC system. PMID:26308851

Properties and characterization of agar/CuNP bionanocomposite films prepared with different copper salts and reducing agents.

PubMed

Shankar, Shiv; Teng, Xinnan; Rhim, Jong-Whan

2014-12-19

Various types of agar-based bio-nanocomposite (BNC) films were prepared by blending agar and six different copper nanoparticles (CuNPs) with different shapes and sizes obtained from three different sources of copper salts and two different reducing agents. The BNC films were characterized by UV-visible, FE-SEM, FT-IR, and XRD. The thermogravimetric study showed that the melting point of BNC films was increased when ascorbic acid was used as a reducing agent for CuNPs synthesis. Apparent surface color and transmittance of agar film was greatly influenced by the reinforcement of CuNPs. However, mechanical and water vapor barrier properties did not change significantly (p>0.05) by blending with CuNPs. Tensile modulus and tensile strength decreased slightly for all types of CuNPs reinforced while elongation at break slightly increased when CuNPs produced by ascorbic acid were blended. The agar bio-nanocomposite films showed profound antibacterial activity against both Gram-positive and Gram-negative food-borne pathogenic bacteria. Copyright © 2014 Elsevier Ltd. All rights reserved.

KSC-07pd3655

NASA Image and Video Library

2007-12-21

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a United Space Alliance technician carefully cuts away the foam insulation surrounding the covers over the feed-through connector box on the external tank for space shuttle Atlantis' STS-122 mission, revealing the fastener holes on the covers. The covers will be removed for access to the feed-through connectors. Following the failure of some of the tank's engine cutoff sensors, or ECO sensors, during propellant tanking for launch attempts on Dec. 6 and Dec. 9, a tanking test was conducted on Dec. 18 to aid in troubleshooting the cause. Technicians spliced test wiring into the ECO sensor electrical system and used time domain reflectometry equipment to help locate the electrical anomaly. Results of the tanking test pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. During the holiday period, workers from Lockheed Martin will begin inspecting and testing the connector. Shuttle program managers will meet on Dec. 27 to review the test and analysis, and decide on a forward plan. Photo credit: NASA/Kim Shiflett

KSC-07pd3654

NASA Image and Video Library

2007-12-21

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a United Space Alliance technician carefully cuts away the foam insulation surrounding the covers over the feed-through connector box on the external tank for space shuttle Atlantis' STS-122 mission, revealing the fastener holes on the covers. The covers will be removed for access to the feed-through connectors. Following the failure of some of the tank's engine cutoff sensors, or ECO sensors, during propellant tanking for launch attempts on Dec. 6 and Dec. 9, a tanking test was conducted on Dec. 18 to aid in troubleshooting the cause. Technicians spliced test wiring into the ECO sensor electrical system and used time domain reflectometry equipment to help locate the electrical anomaly. Results of the tanking test pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. During the holiday period, workers from Lockheed Martin will begin inspecting and testing the connector. Shuttle program managers will meet on Dec. 27 to review the test and analysis, and decide on a forward plan. Photo credit: NASA/Kim Shiflett

KSC-07pd3656

NASA Image and Video Library

2007-12-21

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a United Space Alliance technician removes foam insulation revealing the fastener holes on the covers over the feed-through connector box on the external tank for space shuttle Atlantis' STS-122 mission. The covers will be removed for access to the feed-through connectors. Following the failure of some of the tank's engine cutoff sensors, or ECO sensors, during propellant tanking for launch attempts on Dec. 6 and Dec. 9, a tanking test was conducted on Dec. 18 to aid in troubleshooting the cause. Technicians spliced test wiring into the ECO sensor electrical system and used time domain reflectometry equipment to help locate the electrical anomaly. Results of the tanking test pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. During the holiday period, workers from Lockheed Martin will begin inspecting and testing the connector. Shuttle program managers will meet on Dec. 27 to review the test and analysis, and decide on a forward plan. Photo credit: NASA/Kim Shiflett

KSC-07pd3653

NASA Image and Video Library

2007-12-21

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, the foam insulation surrounding the covers over the feed-through connector box on the external tank for space shuttle Atlantis' STS-122 mission is carefully cut away by a United Space Alliance technician. The covers will be removed for access to the feed-through connectors. Following the failure of some of the tank's engine cutoff sensors, or ECO sensors, during propellant tanking for launch attempts on Dec. 6 and Dec. 9, a tanking test was conducted on Dec. 18 to aid in troubleshooting the cause. Technicians spliced test wiring into the ECO sensor electrical system and used time domain reflectometry equipment to help locate the electrical anomaly. Results of the tanking test pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. During the holiday period, workers from Lockheed Martin will begin inspecting and testing the connector. Shuttle program managers will meet on Dec. 27 to review the test and analysis, and decide on a forward plan. Photo credit: NASA/Kim Shiflett

Epigenetic Testing for Breast Cancer Risk Stratification

DTIC Science & Technology

2012-10-01

The genes selected for this validation were: ER-POS: GSTP1 , HBA2, BNC1, and WDR66 ER-NEG: IRF7, PECI, ARTN, VCAN, ADM, LIPG, and PLAU Figure 1...6 maintained in only a small fraction of the tumor cells. Only BNC1, CCNA1, and GSTP1 show noticeable expansion of the methylated population in...ADM 0.820 0.609 ARTN 0.192 0.138 GSTP1 0.218 0.070 LIPG 0.090 0.011 CCNA1 0.655 0.428 VCAN 0.301 0.128 IRF7 0.483 0.496 HBA2 cND ND PLAU ND ND

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, X.; Yao, C.

A prototype dual-blade stripline kicker for the APS multi-bend achromat (MBA) upgrade has been designed and developed. It was optimized with 3D CST Microwave Studio. The high voltage (HV) feedthrough and air-side connector were designed and optimized. Electromagnetic fields along the beam path, the deflecting angle, the high electric fields and their locations were calculated with 15kV differential pulse voltage applied to the kicker blades through the feedthroughs. Beam impedance and the power dissipation on different parts of the kicker and external loads were studied for a 48-bunch fill pattern. Our results show that the prototype kicker with its HVmore » feedthroughs meets the specified requirements. The results of TDR (time-domain reflectometer) test, high voltage pulse test and beam test of the prototype kicker assembly agreed with the simulations.« less

Method Of Making A Vacuum-Tight Continuous Cable Feedthrough Device

DOEpatents

Bazizi, Kamel Abdel; Haelen, Thomas Eugene; Lobkowicz, Frederick; Slattery, Paul Francis

2001-07-17

A vacuum-tight cable feedthrough device includes a metallic first flange that is penetrated by a slot. Passing through the slot is a flat stripline cable that includes a plurality of conductive signal channels encompassed by a dielectric material on whose upper and lower surfaces is disposed a conductive material includes a ground. The stripline cable is sealed within the slot to provide a substantially vacuum-tight seal between the cable and the first flange. In a preferred embodiment, the cable feedthrough device includes a plurality, at least 16, of stripline cables. In a further preferred embodiment, the device includes a second flange and a bellows sealably connecting the first and second flanges, thereby providing a substantially vacuum-tight, flexible housing for the plurality of cables.

KSC-08pd0038

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- This closeup shows the internal connector to which the replacement feed-through connector in the engine cutoff, or ECO, sensor system on space shuttle Atlantis' external tank will be installed. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

KSC-08pd0045

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- Ray Clark, a Lockheed Martin engineer, completes attaching the replacement feed-through connector in the engine cutoff, or ECO, sensor system to the internal connector on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

KSC-08pd0042

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- Jim Davis, a NASA quality inspector, examines the internal connector on space shuttle Atlantis' external tank to which the replacement feed-through connector in the engine cutoff, or ECO, sensor system will be attached. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

Anomalous NMR Relaxation in Cartilage Matrix Components and Native Cartilage: Fractional-Order Models

PubMed Central

Magin, Richard L.; Li, Weiguo; Velasco, M. Pilar; Trujillo, Juan; Reiter, David A.; Morgenstern, Ashley; Spencer, Richard G.

2011-01-01

We present a fractional-order extension of the Bloch equations to describe anomalous NMR relaxation phenomena (T1 and T2). The model has solutions in the form of Mittag-Leffler and stretched exponential functions that generalize conventional exponential relaxation. Such functions have been shown by others to be useful for describing dielectric and viscoelastic relaxation in complex, heterogeneous materials. Here, we apply these fractional-order T1 and T2 relaxation models to experiments performed at 9.4 and 11.7 Tesla on type I collagen gels, chondroitin sulfate mixtures, and to bovine nasal cartilage (BNC), a largely isotropic and homogeneous form of cartilage. The results show that the fractional-order analysis captures important features of NMR relaxation that are typically described by multi-exponential decay models. We find that the T2 relaxation of BNC can be described in a unique way by a single fractional-order parameter (α), in contrast to the lack of uniqueness of multi-exponential fits in the realistic setting of a finite signal-to-noise ratio. No anomalous behavior of T1 was observed in BNC. In the single-component gels, for T2 measurements, increasing the concentration of the largest components of cartilage matrix, collagen and chondroitin sulfate, results in a decrease in α, reflecting a more restricted aqueous environment. The quality of the curve fits obtained using Mittag-Leffler and stretched exponential functions are in some cases superior to those obtained using mono- and bi-exponential models. In both gels and BNC, α appears to account for microstructural complexity in the setting of an altered distribution of relaxation times. This work suggests the utility of fractional-order models to describe T2 NMR relaxation processes in biological tissues. PMID:21498095

Anomalous NMR relaxation in cartilage matrix components and native cartilage: Fractional-order models

NASA Astrophysics Data System (ADS)

Magin, Richard L.; Li, Weiguo; Pilar Velasco, M.; Trujillo, Juan; Reiter, David A.; Morgenstern, Ashley; Spencer, Richard G.

2011-06-01

We present a fractional-order extension of the Bloch equations to describe anomalous NMR relaxation phenomena ( T1 and T2). The model has solutions in the form of Mittag-Leffler and stretched exponential functions that generalize conventional exponential relaxation. Such functions have been shown by others to be useful for describing dielectric and viscoelastic relaxation in complex, heterogeneous materials. Here, we apply these fractional-order T1 and T2 relaxation models to experiments performed at 9.4 and 11.7 Tesla on type I collagen gels, chondroitin sulfate mixtures, and to bovine nasal cartilage (BNC), a largely isotropic and homogeneous form of cartilage. The results show that the fractional-order analysis captures important features of NMR relaxation that are typically described by multi-exponential decay models. We find that the T2 relaxation of BNC can be described in a unique way by a single fractional-order parameter ( α), in contrast to the lack of uniqueness of multi-exponential fits in the realistic setting of a finite signal-to-noise ratio. No anomalous behavior of T1 was observed in BNC. In the single-component gels, for T2 measurements, increasing the concentration of the largest components of cartilage matrix, collagen and chondroitin sulfate, results in a decrease in α, reflecting a more restricted aqueous environment. The quality of the curve fits obtained using Mittag-Leffler and stretched exponential functions are in some cases superior to those obtained using mono- and bi-exponential models. In both gels and BNC, α appears to account for micro-structural complexity in the setting of an altered distribution of relaxation times. This work suggests the utility of fractional-order models to describe T2 NMR relaxation processes in biological tissues.

Programmable Bio-Nano-Chip Systems for Serum CA125 Quantification: Towards Ovarian Cancer Diagnostics at the Point-of-Care

PubMed Central

Raamanathan, Archana; Simmons, Glennon W.; Christodoulides, Nicolaos; Floriano, Pierre N.; Furmaga, Wieslaw B.; Redding, Spencer W.; Lu, Karen H.; Bast, Robert C.; McDevitt, John T.

2013-01-01

Point-of-care (POC) implementation of early detection and screening methodologies for ovarian cancer may enable improved survival rates through early intervention. Current laboratory-confined immunoanalyzers have long turnaround times and are often incompatible with multiplexing and POC implementation. Rapid, sensitive and multiplexable POC diagnostic platforms compatible with promising early detection approaches for ovarian cancer are needed. To this end, we report the adaptation of the programmable bio-nano-chip (p-BNC), an integrated, microfluidic, modular (Programmable) platform for CA125 serum quantitation, a biomarker prominently implicated in multi-modal and multi-marker screening approaches. In the p-BNC, CA125 from diseased sera (Bio) is sequestered and assessed with a fluorescence-based sandwich immunoassay, completed in the nano-nets (Nano) of sensitized agarose microbeads localized in individually addressable wells (Chip), housed in a microfluidic module, capable of integrating multiple sample, reagent and biowaste processing and handling steps. Antibody pairs that bind to distinct epitopes on CA125 were screened. To permit efficient biomarker sequestration in a 3-D microfluidic environment, the p-BNC operating variables (incubation times, flow rates and reagent concentrations) were tuned to deliver optimal analytical performance under 45 minutes. With short analysis times, competitive analytical performance (Inter- and intra-assay precision of 1.2% and 1.9% and LODs of 1.0 U/mL) was achieved on this mini-sensor ensemble. Further validation with sera of ovarian cancer patients (n=20) demonstrated excellent correlation (R2 = 0.97) with gold-standard ELISA. Building on the integration capabilities of novel microfluidic systems programmed for ovarian cancer, the rapid, precise and sensitive miniaturized p-BNC system shows strong promise for ovarian cancer diagnostics. PMID:22490510

Visual Internal Urethrotomy With Intralesional Mitomycin C and Short-term Clean Intermittent Catheterization for the Management of Recurrent Urethral Strictures and Bladder Neck Contractures.

PubMed

Farrell, Michael R; Sherer, Benjamin A; Levine, Laurence A

2015-06-01

To evaluate our longitudinal experience using visual internal urethrotomy (VIU) with intralesional mitomycin C (MMC) and short-term clean intermittent catheterization (CIC) for urethral strictures and bladder neck contractures (BNC) after failure of endoscopic management. This case series involved review of our prospectively developed database of all men who underwent VIU with MMC and CIC in a standardized fashion for urethral stricture or BNC between 2010 and 2013 at our tertiary care medical center. Etiology was identified as radiation-induced stricture (RIS) or non-RIS and analyzed by stricture location. Cold knife incisions were made in a tri or quadrant fashion followed by intralesional injection of MMC and 1 month of once daily CIC. All 37 patients previously underwent at least 1 intervention for urethral stricture or BNC before VIU with MMC and CIC. Mean stricture length was 2.0 cm (range, 1-6 cm; standard deviation, 1.0 cm). Over the median follow-up period of 23 months (range, 12-39 months), 75.7% of patients required no additional surgical intervention (RIS, 54.5%; non-RIS, 84.6%; P = .051). In those that did recur, median time to stricture recurrence was 8 months (range, 2-28 months). One patient with recurrence required urethroplasty. VIU with MMC followed by short-term CIC provides a minimally invasive and widely available tool to manage complex recurrent urethral strictures (<3 cm) and BNC without significant morbidity. This approach may be most attractive for patients who are poor candidates for open surgery. Copyright © 2015 Elsevier Inc. All rights reserved.

KSC-08pd0066

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, foam is being replaced around the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The foam was removed to enable engineers to remove and replace a feed-through ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0039

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- Mike Berger, with Lockheed Martin, examines the internal connector on space shuttle Atlantis' external tank to which the replacement feed-through connector in the engine cutoff, or ECO, sensor system will be attached. The replacement connector is seen below Berger's hand. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

KSC-08pd0067

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, foam is being replaced around the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The foam was removed to enable engineers to remove and replace a feed-through ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0036

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- This closeup shows the final splicing completed on the wiring between space shuttle Atlantis' external tank and the engine cutoff, or ECO, sensor system before the replacement feed-through connector in the ECO sensor system is installed. Cryogenic shielding is installed around the wiring. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

High voltage variable diameter insulator

DOEpatents

Vanecek, David L.; Pike, Chester D.

1984-01-01

A high voltage feedthrough assembly (10) having a tubular insulator (15) extending between the ground plane ring (16) and the high voltage ring (30). The insulator (15) is made of Pyrex and decreases in diameter from the ground plane ring (16) to the high voltage ring (30), producing equipotential lines almost perpendicular to the wall (27) of the insulator (15) to optimize the voltage-holding capability of the feedthrough assembly (10).

A novel in vitro bovine cartilage punch model for assessing the regeneration of focal cartilage defects with biocompatible bacterial nanocellulose.

PubMed

Pretzel, David; Linss, Stefanie; Ahrem, Hannes; Endres, Michaela; Kaps, Christian; Klemm, Dieter; Kinne, Raimund W

2013-01-01

Current therapies for articular cartilage defects fail to achieve qualitatively sufficient tissue regeneration, possibly because of a mismatch between the speed of cartilage rebuilding and the resorption of degradable implant polymers. The present study focused on the self-healing capacity of resident cartilage cells in conjunction with cell-free and biocompatible (but non-resorbable) bacterial nanocellulose (BNC). This was tested in a novel in vitro bovine cartilage punch model. Standardized bovine cartilage discs with a central defect filled with BNC were cultured for up to eight weeks with/without stimulation with transforming growth factor-β1 (TGF-β1. Cartilage formation and integrity were analyzed by histology, immunohistochemistry and electron microscopy. Content, release and neosynthesis of the matrix molecules proteoglycan/aggrecan, collagen II and collagen I were also quantified. Finally, gene expression of these molecules was profiled in resident chondrocytes and chondrocytes migrated onto the cartilage surface or the implant material. Non-stimulated and especially TGF-β1-stimulated cartilage discs displayed a preserved structural and functional integrity of the chondrocytes and surrounding matrix, remained vital in long-term culture (eight weeks) without signs of degeneration and showed substantial synthesis of cartilage-specific molecules at the protein and mRNA level. Whereas mobilization of chondrocytes from the matrix onto the surface of cartilage and implant was pivotal for successful seeding of cell-free BNC, chondrocytes did not immigrate into the central BNC area, possibly due to the relatively small diameter of its pores (2 to 5 μm). Chondrocytes on the BNC surface showed signs of successful redifferentiation over time, including increase of aggrecan/collagen type II mRNA, decrease of collagen type I mRNA and initial deposition of proteoglycan and collagen type II in long-term high-density pellet cultures. Although TGF-β1 stimulation showed protective effects on matrix integrity, effects on other parameters were limited. The present bovine cartilage punch model represents a robust, reproducible and highly suitable tool for the long-term culture of cartilage, maintaining matrix integrity and homoeostasis. As an alternative to animal studies, this model may closely reflect early stages of cartilage regeneration, allowing the evaluation of promising biomaterials with/without chondrogenic factors.

KSC-07pd3657

NASA Image and Video Library

2007-12-29

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician cuts the external connector cable. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3666

NASA Image and Video Library

2007-12-30

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, technicians wrap the connector for transport to NASA's Marshall Space Flight Center in Huntsville, Ala., for further cryogenic testing. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3658

NASA Image and Video Library

2007-12-29

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician removes a pair of support brackets. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3659

NASA Image and Video Library

2007-12-29

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician gives the connector a cleaning, removing any residual foam insulation, and begins disconnecting the connector assembly. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3665

NASA Image and Video Library

2007-12-30

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, technicians wrap the connector for transport to NASA's Marshall Space Flight Center in Huntsville, Ala., for further cryogenic testing. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3661

NASA Image and Video Library

2007-12-29

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, technicians set up equipment that will be used to take X-rays of the connector cable. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3664

NASA Image and Video Library

2007-12-30

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician inspects the connector just removed from the external tank. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3663

NASA Image and Video Library

2007-12-30

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician demates the connector from the external tank. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3662

NASA Image and Video Library

2007-12-30

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician disconnects the connector before it is demated from the external tank. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3660

NASA Image and Video Library

2007-12-29

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician pulls the connector assembly, with its associated electrical harness, away from the tank. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-08pd0062

NASA Image and Video Library

2008-01-16

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, foam insulation is being trimmed for placement around the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The foam was removed to enable engineers to remove and replace a feed-through ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0064

NASA Image and Video Library

2008-01-16

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, foam insulation is being trimmed for placement around the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The foam was removed to enable engineers to remove and replace a feed-through ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0063

NASA Image and Video Library

2008-01-16

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, foam insulation is being trimmed for placement around the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The foam was removed to enable engineers to remove and replace a feed-through ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0068

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, a technician trims foam placed around the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The foam was removed to enable engineers to remove and replace a feed-through ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0065

NASA Image and Video Library

2008-01-16

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, foam insulation is being trimmed for placement around the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The foam was removed to enable engineers to remove and replace a feed-through ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

Increasing the High Voltage Capabilities and Exploring Parameter Space of an Inertial Electrostatic Confinement Fusion Neutron Source for the Detection of Chemical Explosives

NASA Astrophysics Data System (ADS)

Michalak, Matthew K.

The objectives of the work presented here include understanding key operating principles and providing precise data sets that can be used to test inertial electrostatic confinement (IEC) fusion theory and optimize IEC device operation. The underlying physical behavior was separated from superficial trends observed in an IEC device at the University of Wisconsin-Madison (UW). The effects of changing voltage (30-170 kV) and current (30-100 mA) were thoroughly explored, pressure effects (0.15-1.25 mTorr) were mapped, and the effect of impurities in the system was quantified. The most challenging part of this work was designing a high voltage feedthrough that could reliably operate at higher voltages for far longer times than previously attained. A system to detect conventional explosives using fusion neutrons was also designed, constructed, and tested. Precise data sets were created by taking into account and minimizing the effects of short and long term trends in the experiment. Detailed meter current scans were taken that showed a linear relationship of the neutron production rate with current. Cathode voltage scans were slightly greater than linear in the neutron rate from 30 to 170 kV, but the rate increase diminished to near linear as 170 kV was approached. A new high voltage feedthrough was designed that surpassed the performance of past UW IEC lab feedthroughs and shows promise for long duration operation at still higher voltages. Limitations of other equipment in the IEC lab prevented testing the feedthrough to voltages above 175 kV. A more robust construction of the feedthrough and reducing the consequences of a feedthrough failure were also important design criteria that were met. A detector array was made to detect explosives via the 10.8 MeV neutron capture prompt gamma from nitrogen. Signals from four separate detectors were combined to make the individual detectors act similar to one large detector. The detector signals were both summed and combined to compare the performance of the two methods. An overwhelming background radiation signal and insufficient time resolution were two factors that led to the combined signal not performing as well as the summed signal.

Single electron beam rf feedback free electron laser

DOEpatents

Brau, C.A.; Stein, W.E.; Rockwood, S.D.

1981-02-11

A free electron laser system and electron beam system for a free electron laser which uses rf feedback to enhance efficiency are described. Rf energy is extracted from a single electron beam by decelerating cavities and energy is returned to accelerating cavities using rf returns, such as rf waveguides, rf feedthroughs, resonant feedthroughs, etc. This rf energy is added to rf klystron energy to reduce the required input energy and thereby enhance energy efficiency of the system.

A biodynamic feedthrough model based on neuromuscular principles.

PubMed

Venrooij, Joost; Abbink, David A; Mulder, Mark; van Paassen, Marinus M; Mulder, Max; van der Helm, Frans C T; Bulthoff, Heinrich H

2014-07-01

A biodynamic feedthrough (BDFT) model is proposed that describes how vehicle accelerations feed through the human body, causing involuntary limb motions and so involuntary control inputs. BDFT dynamics strongly depend on limb dynamics, which can vary between persons (between-subject variability), but also within one person over time, e.g., due to the control task performed (within-subject variability). The proposed BDFT model is based on physical neuromuscular principles and is derived from an established admittance model-describing limb dynamics-which was extended to include control device dynamics and account for acceleration effects. The resulting BDFT model serves primarily the purpose of increasing the understanding of the relationship between neuromuscular admittance and biodynamic feedthrough. An added advantage of the proposed model is that its parameters can be estimated using a two-stage approach, making the parameter estimation more robust, as the procedure is largely based on the well documented procedure required for the admittance model. To estimate the parameter values of the BDFT model, data are used from an experiment in which both neuromuscular admittance and biodynamic feedthrough are measured. The quality of the BDFT model is evaluated in the frequency and time domain. Results provide strong evidence that the BDFT model and the proposed method of parameter estimation put forward in this paper allows for accurate BDFT modeling across different subjects (accounting for between-subject variability) and across control tasks (accounting for within-subject variability).

Substrate Specificities and Conformational Flexibility of 3-Ketosteroid 9α-Hydroxylases*

PubMed Central

Penfield, Jonathan S.; Worrall, Liam J.; Strynadka, Natalie C.; Eltis, Lindsay D.

2014-01-01

KshA is the oxygenase component of 3-ketosteroid 9α-hydroxylase, a Rieske oxygenase involved in the bacterial degradation of steroids. Consistent with its role in bile acid catabolism, KshA1 from Rhodococcus rhodochrous DSM43269 had the highest apparent specificity (kcat/Km) for steroids with an isopropyl side chain at C17, such as 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oate (1,4-BNC). By contrast, the KshA5 homolog had the highest apparent specificity for substrates with no C17 side chain (kcat/Km >105 s−1 m−1 for 4-estrendione, 5α-androstandione, and testosterone). Unexpectedly, substrates such as 4-androstene-3,17-dione (ADD) and 4-BNC displayed strong substrate inhibition (KiS ∼100 μm). By comparison, the cholesterol-degrading KshAMtb from Mycobacterium tuberculosis had the highest specificity for CoA-thioesterified substrates. These specificities are consistent with differences in the catabolism of cholesterol and bile acids, respectively, in actinobacteria. X-ray crystallographic structures of the KshAMtb·ADD, KshA1·1,4-BNC-CoA, KshA5·ADD, and KshA5·1,4-BNC-CoA complexes revealed that the enzymes have very similar steroid-binding pockets with the substrate's C17 oriented toward the active site opening. Comparisons suggest Tyr-245 and Phe-297 are determinants of KshA1 specificity. All enzymes have a flexible 16-residue “mouth loop,” which in some structures completely occluded the substrate-binding pocket from the bulk solvent. Remarkably, the catalytic iron and α-helices harboring its ligands were displaced up to 4.4 Å in the KshA5·substrate complexes as compared with substrate-free KshA, suggesting that Rieske oxygenases may have a dynamic nature similar to cytochrome P450. PMID:25049233

Activity of 3-Ketosteroid 9α-Hydroxylase (KshAB) Indicates Cholesterol Side Chain and Ring Degradation Occur Simultaneously in Mycobacterium tuberculosis*

PubMed Central

Capyk, Jenna K.; Casabon, Israël; Gruninger, Robert; Strynadka, Natalie C.; Eltis, Lindsay D.

2011-01-01

Mycobacterium tuberculosis (Mtb), a significant global pathogen, contains a cholesterol catabolic pathway. Although the precise role of cholesterol catabolism in Mtb remains unclear, the Rieske monooxygenase in this pathway, 3-ketosteroid 9α-hydroxylase (KshAB), has been identified as a virulence factor. To investigate the physiological substrate of KshAB, a rhodococcal acyl-CoA synthetase was used to produce the coenzyme A thioesters of two cholesterol derivatives: 3-oxo-23,24-bisnorchol-4-en-22-oic acid (forming 4-BNC-CoA) and 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (forming 1,4-BNC-CoA). The apparent specificity constant (kcat/Km) of KshAB for the CoA thioester substrates was 20–30 times that for the corresponding 17-keto compounds previously proposed as physiological substrates. The apparent KmO2 was 90 ± 10 μm in the presence of 1,4-BNC-CoA, consistent with the value for two other cholesterol catabolic oxygenases. The Δ1 ketosteroid dehydrogenase KstD acted with KshAB to cleave steroid ring B with a specific activity eight times greater for a CoA thioester than the corresponding ketone. Finally, modeling 1,4-BNC-CoA into the KshA crystal structure suggested that the CoA moiety binds in a pocket at the mouth of the active site channel and could contribute to substrate specificity. These results indicate that the physiological substrates of KshAB are CoA thioester intermediates of cholesterol side chain degradation and that side chain and ring degradation occur concurrently in Mtb. This finding has implications for steroid metabolites potentially released by the pathogen during infection and for the design of inhibitors for cholesterol-degrading enzymes. The methodologies and rhodococcal enzymes used to generate thioesters will facilitate the further study of cholesterol catabolism. PMID:21987574

Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis.

PubMed

Capyk, Jenna K; Casabon, Israël; Gruninger, Robert; Strynadka, Natalie C; Eltis, Lindsay D

2011-11-25

Mycobacterium tuberculosis (Mtb), a significant global pathogen, contains a cholesterol catabolic pathway. Although the precise role of cholesterol catabolism in Mtb remains unclear, the Rieske monooxygenase in this pathway, 3-ketosteroid 9α-hydroxylase (KshAB), has been identified as a virulence factor. To investigate the physiological substrate of KshAB, a rhodococcal acyl-CoA synthetase was used to produce the coenzyme A thioesters of two cholesterol derivatives: 3-oxo-23,24-bisnorchol-4-en-22-oic acid (forming 4-BNC-CoA) and 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (forming 1,4-BNC-CoA). The apparent specificity constant (k(cat)/K(m)) of KshAB for the CoA thioester substrates was 20-30 times that for the corresponding 17-keto compounds previously proposed as physiological substrates. The apparent K(m)(O(2)) was 90 ± 10 μM in the presence of 1,4-BNC-CoA, consistent with the value for two other cholesterol catabolic oxygenases. The Δ(1) ketosteroid dehydrogenase KstD acted with KshAB to cleave steroid ring B with a specific activity eight times greater for a CoA thioester than the corresponding ketone. Finally, modeling 1,4-BNC-CoA into the KshA crystal structure suggested that the CoA moiety binds in a pocket at the mouth of the active site channel and could contribute to substrate specificity. These results indicate that the physiological substrates of KshAB are CoA thioester intermediates of cholesterol side chain degradation and that side chain and ring degradation occur concurrently in Mtb. This finding has implications for steroid metabolites potentially released by the pathogen during infection and for the design of inhibitors for cholesterol-degrading enzymes. The methodologies and rhodococcal enzymes used to generate thioesters will facilitate the further study of cholesterol catabolism.

KSC-07pd3667

NASA Image and Video Library

2007-12-30

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, technicians place the wrapped connector in a shipping container for transport to NASA's Marshall Space Flight Center in Huntsville, Ala., for further cryogenic testing. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-07pd3668

NASA Image and Video Library

2007-12-30

KENNEDY SPACE CENTER, FLA. -- At Launch Pad 39A, a team of external tank specialists from Lockheed Martin and the United Space Alliance undertakes the task of removing the hydrogen feed-through connector in support of space shuttle Atlantis' STS-122 mission. Here, a technician carries the shipping container in which the wrapped connector is secured for transport to NASA's Marshall Space Flight Center in Huntsville, Ala., for further cryogenic testing. Some of the tank's engine cutoff sensors, or ECO sensors, failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the data from additional testing on the connector is analyzed, shuttle program managers will decide on a forward plan. Launch of STS-122 is targeted for January 2008. Photo credit: NASA/George Shelton

KSC-08pd0037

NASA Image and Video Library

2008-01-13

KENNEDY SPACE CENTER, FLA. -- Lloyd Johns, with Lockheed Martin, prepares the area where the replacement feed-through connector in the engine cutoff, or ECO, sensor system on space shuttle Atlantis' external tank will be installed. Seen next to Johns' arm is the internal connector. The area is wrapped in material for weather protection. Berger wears safety equipment as helium gas is employed to keep the equipment under wrap dry. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The work is being done on Launch Pad 39A. Space shuttle Atlantis is now targeted for launch on Feb. 7. Photo credit: NASA/George Shelton

A facile method to modify bentonite nanoclay with silane

NASA Astrophysics Data System (ADS)

Abeywardena, Sujani B. Y.; Perera, Srimala; Nalin de Silva, K. M.; Tissera, Nadeeka P.

2017-07-01

Immobilization of smectite clay onto a desirable surface has received much attention, since its nanospace can be utilized for many applications in material science. Here, we present an efficient method to functionalize surface of bentonite nanoclay (BNC) through the grafting of 3-aminotriethoxysilane (APTES). Infrared spectroscopy and elemental analysis confirmed the presence of organic chains and amine groups in modified nanoclay. XRD analysis confirmed grafting of APTES on the surface of bentonite nanoclay without intercalation. The accomplishment of the surface modification was quantitatively proved by TGA analysis. Modified BNC can covalently couple with different material surfaces, allowing its nanospace to be utilized for intercalation of cations, bio-molecules, and polymeric materials, to be used in advanced military aerospace, pharmaceuticals, and many other commercial applications.

Signs of atopic dermatitis and contact dermatitis affected by distinct H2-haplotype in the NC/Nga genetic background.

PubMed

Ohkusu-Tsukada, Kozo; Ito, Daiki; Okuno, Yuki; Tsukada, Teruyo; Takahashi, Kimimasa

2018-02-07

We recently advocated in favour of naming a novel H2-haplotype consisting of K d , D/L dm7 , I-A k and I-E k in the atopic dermatitis (AD) mouse model NC/Nga as "H-2 nc ." The role of the H2-haplotype in AD development was investigated in H2 b -congenic NC/Nga mice (NC.h2 b/b and NC.h2 b/nc ) established by backcrossing. A severe 2,4-dinitrofluorobenzene (DNFB)-induced dermatitis in NC/Nga was alleviated partially in NC.h2 b/nc and significantly in NC.h2 b/b . The AD phenotype was correlated with thymic stromal lymphopoietin (TSLP)-epidermal expression levels and serum levels of total IgE and IL-18/IL-33. Histologically, allergic contact dermatitis (ACD) was accompanied by lymphocytes and plasma cells-infiltrating perivasculitis in NC.h2 b/nc and NC.h2 b/b and clearly differed from AD accompanied by neutrophils, eosinophils and macrophages-infiltrating diffuse suppurative dermatitis in NC/Nga. Interestingly, IFN-γ/IL-17 production from autoreactive CD4 + T-cells remarkably increased in DNFB-sensitised NC.h2 b/b but not in NC/Nga. Our findings suggest that AD or ACD may depend on haplotype H-2 nc or H-2 b , respectively, in addition to the NC/Nga genetic background.

High voltage testing for the Majorana Demonstrator

DOE PAGES

Abgrall, N.; Arnquist, I. J.; Avignone, III, F. T.; ...

2016-04-04

The Majorana Collaboration is constructing the Majorana Demonstrator, an ultra-low background, 44-kg modular high-purity Ge (HPGe) detector array to search for neutrinoless double-beta decay in 76Ge. The phenomenon of surface micro-discharge induced by high-voltage has been studied in the context of the Majorana Demonstrator. This effect can damage the front-end electronics or mimic detector signals. To ensure the correct performance, every high-voltage cable and feedthrough must be capable of supplying HPGe detector operating voltages as high as 5 kV without exhibiting discharge. R&D measurements were carried out to understand the testing system and determine the optimum design configuration of themore » high-voltage path, including different improvements of the cable layout and feedthrough flange model selection. Every cable and feedthrough to be used at the Majorana Demonstrator was characterized and the micro-discharge effects during the Majorana Demonstrator commissioning phase were studied. Furthermore, a stable configuration has been achieved, and the cables and connectors can supply HPGe detector operating voltages without exhibiting discharge.« less

High voltage testing for the Majorana Demonstrator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abgrall, N.; Arnquist, Isaac J.; Avignone, F. T.

2016-07-01

The Majorana Collaboration is constructing theMajorana Demonstrator, an ultra-low background, 44-kg modular high-purity Ge (HPGe) detector array to search for neutrinoless double-beta decay in 76Ge. The phenomenon of surface micro-discharge induced by high-voltage has been studied in the context of theMajorana Demonstrator. This effect can damage the front-end electronics or mimic detector signals. To ensure the correct performance, every high-voltage cable and feedthrough must be capable of supplying HPGe detector operating voltages as high as 5 kV without exhibiting discharge. R&D measurements were carried out to understand the testing system and determine the optimum design configuration of the high-voltage path,more » including different improvements of the cable layout and feedthrough flange model selection. Every cable and feedthrough to be used at the Majorana Demonstrator was characterized and the micro-discharge effects during theMajorana Demonstrator commissioning phase were studied. A stable configuration has been achieved, and the cables and connectors can supply HPGe detector operating voltages without exhibiting discharge.« less

KSC-08pd0078

NASA Image and Video Library

2008-01-23

KENNEDY SPACE CENTER, FLA. -- Quality inspectors with NASA and Lockheed Martin examine a red-line drawing of foam placement on space shuttle Atlantis's external tank (in front of them) to verify the foam insulation that was reapplied. The foam covers the feed-through engine cut-off, or ECO, sensor connector. The foam was removed to enable engineers to remove and replace the ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Cory Husten

KSC-08pd0033

NASA Image and Video Library

2008-01-11

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A, Lockheed Martin engineer Ray Clark splices wires between space shuttle Atlantis' external tank and the engine cutoff, or ECO, sensor system. The replacement feed-through connector in the ECO sensor system will be installed later. Some of the tank's ECO sensors gave failed readings during propellant tanking for Atlantis' STS-122 mission launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. No problems with the ECO sensors themselves have been found. NASA's Space Shuttle Program has proposed a target launch date of Feb. 7 for the STS-122 mission. That proposed launch date remains under evaluation pending coordination with all partners in the International Space Station Program. Photo credit: NASA/George Shelton

KSC-08pd0077

NASA Image and Video Library

2008-01-23

KENNEDY SPACE CENTER, FLA. -- Quality inspectors with NASA and Lockheed Martin examine a red-line drawing of foam placement on space shuttle Atlantis's external tank (in front of them) to verify the foam insulation that was reapplied. The foam covers the feed-through engine cut-off, or ECO, sensor connector. The foam was removed to enable engineers to remove and replace the ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Cory Husten

KSC-08pd0008

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, the first two of nine pins have been soldered to the socket of the replacement feed-through connector that will be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. Two United Launch Alliance technicians, who performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994, will be doing the soldering. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

KSC-08pd0007

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, the first two of nine pins have been soldered to the socket of the replacement feed-through connector that will be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. Two United Launch Alliance technicians, who performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994, will be doing the soldering. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

KSC-08pd0076

NASA Image and Video Library

2008-01-23

KENNEDY SPACE CENTER, FLA. -- The white foam insulation freshly reapplied to space shuttle Atlantis's external tank completes the work to remove and replace the feed-through connector on the engine cut-off, or ECO, sensor connector. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The foam was removed to enable engineers to remove and replace the ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Cory Husten

KSC-08pd0035

NASA Image and Video Library

2008-01-11

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A, Lockheed Martin engineer Ray Clark splices wires between space shuttle Atlantis' external tank and the engine cutoff, or ECO, sensor system. The replacement feed-through connector in the ECO sensor system will be installed later. Some of the tank's ECO sensors gave failed readings during propellant tanking for Atlantis' STS-122 mission launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. No problems with the ECO sensors themselves have been found. NASA's Space Shuttle Program has proposed a target launch date of Feb. 7 for the STS-122 mission. That proposed launch date remains under evaluation pending coordination with all partners in the International Space Station Program. Photo credit: NASA/George Shelton

KSC-08pd0080

NASA Image and Video Library

2008-01-23

KENNEDY SPACE CENTER, FLA. -- Quality inspectors with NASA and Lockheed Martin examine a red-line drawing of foam placement on space shuttle Atlantis's external tank (in front of them) to verify the foam insulation that was reapplied. The foam covers the feed-through engine cut-off, or ECO, sensor connector. The foam was removed to enable engineers to remove and replace the ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Cory Husten

KSC-08pd0034

NASA Image and Video Library

2008-01-11

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A, Lockheed Martin engineer Ray Clark splices wires between space shuttle Atlantis' external tank and the engine cutoff, or ECO, sensor system. The replacement feed-through connector in the ECO sensor system will be installed later. Some of the tank's ECO sensors gave failed readings during propellant tanking for Atlantis' STS-122 mission launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. The pins in the replacement connector have been precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. No problems with the ECO sensors themselves have been found. NASA's Space Shuttle Program has proposed a target launch date of Feb. 7 for the STS-122 mission. That proposed launch date remains under evaluation pending coordination with all partners in the International Space Station Program. Photo credit: NASA/George Shelton

KSC-08pd0075

NASA Image and Video Library

2008-01-23

KENNEDY SPACE CENTER, FLA. -- The white foam insulation freshly reapplied to space shuttle Atlantis's external tank completes the work to remove and replace the feed-through connector on the engine cut-off, or ECO, sensor connector. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The foam was removed to enable engineers to remove and replace the ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Cory Husten

KSC-08pd0079

NASA Image and Video Library

2008-01-23

KENNEDY SPACE CENTER, FLA. -- Quality inspectors with NASA and Lockheed Martin examine a red-line drawing of foam placement on space shuttle Atlantis's external tank (in front of them) to verify the foam insulation that was reapplied. The foam covers the feed-through engine cut-off, or ECO, sensor connector. The foam was removed to enable engineers to remove and replace the ECO sensor connector on the tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The repair work was done on Atlantis while the shuttle has been on Launch Pad 39A at NASA's Kennedy Space Center. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Cory Husten

Adaptive piezoelectric sensoriactuator

NASA Technical Reports Server (NTRS)

Clark, Jr., Robert L. (Inventor); Vipperman, Jeffrey S. (Inventor); Cole, Daniel G. (Inventor)

1996-01-01

An adaptive algorithm implemented in digital or analog form is used in conjunction with a voltage controlled amplifier to compensate for the feedthrough capacitance of piezoelectric sensoriactuator. The mechanical response of the piezoelectric sensoriactuator is resolved from the electrical response by adaptively altering the gain imposed on the electrical circuit used for compensation. For wideband, stochastic input disturbances, the feedthrough capacitance of the sensoriactuator can be identified on-line, providing a means of implementing direct-rate-feedback control in analog hardware. The device is capable of on-line system health monitoring since a quasi-stable dynamic capacitance is indicative of sustained health of the piezoelectric element.

Next Generation Programmable Bio-Nano-Chip System for On-Site Detection in Oral Fluids.

PubMed

Christodoulides, Nicolaos; De La Garza, Richard; Simmons, Glennon W; McRae, Michael P; Wong, Jorge; Newton, Thomas F; Kosten, Thomas R; Haque, Ahmed; McDevitt, John T

2015-11-23

Current on-site drug of abuse detection methods involve invasive sampling of blood and urine specimens, or collection of oral fluid, followed by qualitative screening tests using immunochromatographic cartridges. Test confirmation and quantitative assessment of a presumptive positive are then provided by remote laboratories, an inefficient and costly process decoupled from the initial sampling. Recently, a new noninvasive oral fluid sampling approach that is integrated with the chip-based Programmable Bio-Nano-Chip (p-BNC) platform has been developed for the rapid (~ 10 minutes), sensitive detection (~ ng/ml) and quantitation of 12 drugs of abuse. Furthermore, the system can provide the time-course of select drug and metabolite profiles in oral fluids. For cocaine, we observed three slope components were correlated with cocaine-induced impairment using this chip-based p-BNC detection modality. Thus, this p-BNC has significant potential for roadside drug testing by law enforcement officers. Initial work reported on chip-based drug detection was completed using 'macro' or "chip in the lab" prototypes, that included metal encased "flow cells", external peristaltic pumps and a bench-top analyzer system instrumentation. We now describe the next generation miniaturized analyzer instrumentation along with customized disposables and sampling devices. These tools will offer real-time oral fluid drug monitoring capabilities, to be used for roadside drug testing as well as testing in clinical settings as a non-invasive, quantitative, accurate and sensitive tool to verify patient adherence to treatment.

Atomically thin layers of B-N-C-O with tunable composition.

PubMed

Ozturk, Birol; de-Luna-Bugallo, Andres; Panaitescu, Eugen; Chiaramonti, Ann N; Liu, Fangze; Vargas, Anthony; Jiang, Xueping; Kharche, Neerav; Yavuzcetin, Ozgur; Alnaji, Majed; Ford, Matthew J; Lok, Jay; Zhao, Yongyi; King, Nicholas; Dhar, Nibir K; Dubey, Madan; Nayak, Saroj K; Sridhar, Srinivas; Kar, Swastik

2015-07-01

In recent times, atomically thin alloys of boron, nitrogen, and carbon have generated significant excitement as a composition-tunable two-dimensional (2D) material that demonstrates rich physics as well as application potentials. The possibility of tunably incorporating oxygen, a group VI element, into the honeycomb sp(2)-type 2D-BNC lattice is an intriguing idea from both fundamental and applied perspectives. We present the first report on an atomically thin quaternary alloy of boron, nitrogen, carbon, and oxygen (2D-BNCO). Our experiments suggest, and density functional theory (DFT) calculations corroborate, stable configurations of a honeycomb 2D-BNCO lattice. We observe micrometer-scale 2D-BNCO domains within a graphene-rich 2D-BNC matrix, and are able to control the area coverage and relative composition of these domains by varying the oxygen content in the growth setup. Macroscopic samples comprising 2D-BNCO domains in a graphene-rich 2D-BNC matrix show graphene-like gate-modulated electronic transport with mobility exceeding 500 cm(2) V(-1) s(-1), and Arrhenius-like activated temperature dependence. Spin-polarized DFT calculations for nanoscale 2D-BNCO patches predict magnetic ground states originating from the B atoms closest to the O atoms and sizable (0.6 eV < E g < 0.8 eV) band gaps in their density of states. These results suggest that 2D-BNCO with novel electronic and magnetic properties have great potential for nanoelectronics and spintronic applications in an atomically thin platform.

Gram-scale production of B, N co-doped graphene-like carbon for high performance supercapacitor electrodes

NASA Astrophysics Data System (ADS)

Chen, Zhuo; Hou, Liqiang; Cao, Yan; Tang, Yushu; Li, Yongfeng

2018-03-01

Boron and nitrogen co-doped graphene-like carbon (BNC) with a gram scale was synthesized via a two-step method including a ball-milling process and a calcination process and used as electrode materials for supercapacitors. High surface area and abundant active sites of graphene-like carbon were created by the ball-milling process. Interestingly, the nitrogen atoms are doped in carbon matrix without any other N sources except for air. The textual and chemical properties can be easily tuned by changing the calcination temperature, and at 900 oC the BNC with a high surface area (802.35 m2/g), a high boron content (2.19 at%), a hierarchical pore size distribution and a relatively high graphitic degree was obtained. It shows an excellent performance of high specific capacitance retention about 78.2% at high current density (199 F/g at 100 A/g) of the initial capacitance (254 F/g at 0.25 A/g) and good cycling stability (90% capacitance retention over 1000 cycles at 100 A/g) measured in a three-electrode system. Furthermore, in a two-electrode system, a specific capacitance of 225 F/g at 0.25 A/g and a good cycling stability (93% capacitance retention over 20,000 cycles at 25 A/g) were achieved by using BNC as electrodes. The strategy of synthesis is facile and effective to fabricate multi-doped graphene-like carbon for promising candidates as electrode materials in supercapacitors.

Dimensions and Measurements of Debuncher Band 1 and 2 Waveguide-Coax Launchers (Final Version)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, Ding; /Fermilab

2000-02-15

This note is a document about dimensions and measurement results of waveguide-coax launchers (Band 1 and 2) installed on the arrays in debuncher cooling upgrade. Shown in Figure 1, 5, 8 and 12 are schematic drawings of launchers in the cross section along the longitudinal direction (beam direction) of the arrays. The unit in these drawings is inch. Note: although there are upper band and lower band for pickup arrays, the launchers are the same to avoid possible confusion during installation. RF Measurements were made on all launchers (port) and printed in hard copies for future reference. Since the measurementmore » results are similar to each other, only a few plots for each type of launcher/band are presented in this document. There are two types of measured S11 parameters. One is the measurement made at the end of design/tuning stage using a straight section of band 1 or 2 waveguide terminated with a cone of absorber. I use 'Original' to denote this kind of measurement. As shown in Figure 2, 6, 9 and 13, the original S11 of all launchers are below or around - 20 db over the full band 1 or 2. The other type of measurement is the one made after these launchers were installed onto the array including elbows and several type N feedthrough or connectors. The kicker arrays were terminated with wedges of absorber. During all measurements (pickup array or kicker array) when one launcher was being measured, all other launchers were terminated with 50 ohm terminator. As shown in Figure 3, 4, 7, 10, 11 and 14 these 'final' S11s are around -15 db.« less

Correction for Thermal EMFs in Thermocouple Feedthroughs

NASA Technical Reports Server (NTRS)

Ziemke, Robert A.

2006-01-01

A straightforward measurement technique provides for correction of thermal-electromotive-force (thermal-EMF) errors introduced by temperature gradients along the pins of non-thermocouple-alloy hermetic feedthrough connectors for thermocouple extension wires that must pass through bulkheads. This technique is an alternative to the traditional technique in which the thermal-EMF errors are eliminated by use of custom-made multipin hermetic feedthrough connectors that contain pins made of the same alloys as those of the thermocouple extension wires. One disadvantage of the traditional technique is that it is expensive and time-consuming to fabricate multipin custom thermocouple connectors. In addition, the thermocouple-alloy pins in these connectors tend to corrode easily and/or tend to be less rugged compared to the non-thermocouple-alloy pins of ordinary connectors. As the number of thermocouples (and thus pins) is increased in a given setup, the magnitude of these disadvantages increases accordingly. The present technique is implemented by means of a little additional hardware and software, the cost of which is more than offset by the savings incurred through the use of ordinary instead of thermocouple connectors. The figure schematically depicts a typical measurement setup to which the technique is applied. The additional hardware includes an isothermal block (made of copper) instrumented with a reference thermocouple and a compensation thermocouple. The reference thermocouple is connected to an external data-acquisition system (DAS) through a two-pin thermocouple-alloy hermetic feedthrough connector, but this is the only such connector in the apparatus. The compensation thermocouple is connected to the DAS through two pins of the same ordinary multipin connector that connects the measurement thermocouples to the DAS.

High accuracy switched-current circuits using an improved dynamic mirror

NASA Technical Reports Server (NTRS)

Zweigle, G.; Fiez, T.

1991-01-01

The switched-current technique, a recently developed circuit approach to analog signal processing, has emerged as an alternative/compliment to the well established switched-capacitor circuit technique. High speed switched-current circuits offer potential cost and power savings over slower switched-capacitor circuits. Accuracy improvements are a primary concern at this stage in the development of the switched-current technique. Use of the dynamic current mirror has produced circuits that are insensitive to transistor matching errors. The dynamic current mirror has been limited by other sources of error including clock-feedthrough and voltage transient errors. In this paper we present an improved switched-current building block using the dynamic current mirror. Utilizing current feedback the errors due to current imbalance in the dynamic current mirror are reduced. Simulations indicate that this feedback can reduce total harmonic distortion by as much as 9 dB. Additionally, we have developed a clock-feedthrough reduction scheme for which simulations reveal a potential 10 dB total harmonic distortion improvement. The clock-feedthrough reduction scheme also significantly reduces offset errors and allows for cancellation with a constant current source. Experimental results confirm the simulated improvements.

KSC-08pd0006

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, Bob Arp, an aerospace technician with the United Launch Alliance, solders a pin to the socket of the replacement feed-through connector that will be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. The technician performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994 and was specifically chosen for the task. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

KSC-08pd0004

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, aerospace technicians with the United Launch Alliance inspect an electrical wiring harness that has been inserted into a replacement feed-through connector during preparations to solder the pins to the socket of the connector that will be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. The technicians performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994 and were specifically chosen for the task. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

KSC-08pd0001

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, a Lockheed Martin technician prepares an electrical wiring harness during a procedure to solder the pins to the socket of the replacement feed-through connector that will be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. Two United Launch Alliance technicians, who performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994, will be doing the soldering. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

KSC-08pd0009

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, Bob Arp, an aerospace technician with the United Launch Alliance, examinies the pins remaining to be soldered to the socket of the replacement feed-through connector that will be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. The technician performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994 and was specifically chosen for the task. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

KSC-08pd0003

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, Kevin Wyckoff, an aerospace technician with the United Launch Alliance, inserts an electrical wiring harness into a replacement feed-through connector during preparations to solder the pins to the socket of the connector. The connector will later be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. The technician performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994 and was specifically chosen for the task. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

A framework for biodynamic feedthrough analysis--part I: theoretical foundations.

PubMed

Venrooij, Joost; van Paassen, Marinus M; Mulder, Mark; Abbink, David A; Mulder, Max; van der Helm, Frans C T; Bulthoff, Heinrich H

2014-09-01

Biodynamic feedthrough (BDFT) is a complex phenomenon, which has been studied for several decades. However, there is little consensus on how to approach the BDFT problem in terms of definitions, nomenclature, and mathematical descriptions. In this paper, a framework for biodynamic feedthrough analysis is presented. The goal of this framework is two-fold. First, it provides some common ground between the seemingly large range of different approaches existing in the BDFT literature. Second, the framework itself allows for gaining new insights into BDFT phenomena. It will be shown how relevant signals can be obtained from measurement, how different BDFT dynamics can be derived from them, and how these different dynamics are related. Using the framework, BDFT can be dissected into several dynamical relationships, each relevant in understanding BDFT phenomena in more detail. The presentation of the BDFT framework is divided into two parts. This paper, Part I, addresses the theoretical foundations of the framework. Part II, which is also published in this issue, addresses the validation of the framework. The work is presented in two separate papers to allow for a detailed discussion of both the framework's theoretical background and its validation.

A network model of correlated growth of tissue stiffening in pulmonary fibrosis

NASA Astrophysics Data System (ADS)

Oliveira, Cláudio L. N.; Bates, Jason H. T.; Suki, Béla

2014-06-01

During the progression of pulmonary fibrosis, initially isolated regions of high stiffness form and grow in the lung tissue due to collagen deposition by fibroblast cells. We have previously shown that ongoing collagen deposition may not lead to significant increases in the bulk modulus of the lung until these local remodeled regions have become sufficiently numerous and extensive to percolate in a continuous path across the entire tissue (Bates et al 2007 Am. J. Respir. Crit. Care Med. 176 617). This model, however, did not include the possibility of spatially correlated deposition of collagen. In the present study, we investigate whether spatial correlations influence the bulk modulus in a two-dimensional elastic network model of lung tissue. Random collagen deposition at a single site is modeled by increasing the elastic constant of the spring at that site by a factor of 100. By contrast, correlated collagen deposition is represented by stiffening the springs encountered along a random walk starting from some initial spring, the rationale being that excess collagen deposition is more likely in the vicinity of an already stiff region. A combination of random and correlated deposition is modeled by performing random walks of length N from randomly selected initial sites, the balance between the two processes being determined by N. We found that the dependence of bulk modulus, B(N,c), on both N and the fraction of stiff springs, c, can be described by a strikingly simple set of empirical equations. For c<0.3, B(N,c) exhibits exponential growth from its initial value according to B(N,c)\\approx {{B}_{0}}exp (2c)\\left[ 1+{{c}^{\\beta }}ln \\left( {{N}^{{{a}_{I}}}} \\right) \\right], where \\beta =0.994+/- 0.024 and {{a}_{I}}=0.54+/- 0.026. For intermediate concentrations of stiffening, 0.3\\leqslant c\\leqslant 0.8, another exponential rule describes the bulk modulus as B(N,c)=4{{B}_{0}}exp \\left[ {{a}_{II}}\\left( c-{{c}_{c}} \\right) \\right], where {{a}_{II}} and {{c}_{c}} are parameters that depend on N. For c>0.8, B(N,c) is linear in c and independent of N, such that B(N,c)=100\\;{{B}_{0}}-100{{a}_{III}}(1-c){{B}_{0}}, where {{a}_{III}}=2.857. For small concentrations, the physiologically most relevant regime, the forces in the network springs are distributed according to a power law. When c = 0.3, the exponent of this power law increases from -4.5, when N = 1, and saturates to about -2, as N increases above 40. These results suggest that the spatial correlation of collagen deposition in the fibrotic lung has a strong effect on the rate of lung function decline and on the mechanical environment in which the cells responsible for remodeling find themselves.

BN-C Hybrid Nanoribbons as Gas Sensors

NASA Astrophysics Data System (ADS)

Darvishi Gilan, Mahdi; Chegel, Raad

2018-02-01

The effects of carbon monoxide (CO) and ammonia (NH3) molecules adsorption on the various composites of boron nitride and graphene BN-C hybrid nanoribbons are investigated using the non-equilibrium Green's function (NEGF) technique based on density functional theory (DFT). The effects of adsorption with possible random configurations on the average of the density of states (DOS), transmission coefficient, and the current-voltage ( I- V) characteristics are calculated. The results indicate that, by embedding armchair graphene nanoribbon (AGNR) with boron nitride nanoribbon (BNNR), the various electronic properties can be observed after gas molecule adsorption. The electronic structure and gap of hybrids system is modified due to gas adsorption, and the systems act like the n-type semiconductor by NH3 molecule adsorption. The hybrid structures due to their tunable band gap are better candidates for gas detecting compared to the pristine BNNRs and AGNRs.

A Genome-Wide Association Study Identifies the Skin Color Genes IRF4, MC1R, ASIP, and BNC2 Influencing Facial Pigmented Spots.

PubMed

Jacobs, Leonie C; Hamer, Merel A; Gunn, David A; Deelen, Joris; Lall, Jaspal S; van Heemst, Diana; Uh, Hae-Won; Hofman, Albert; Uitterlinden, André G; Griffiths, Christopher E M; Beekman, Marian; Slagboom, P Eline; Kayser, Manfred; Liu, Fan; Nijsten, Tamar

2015-07-01

Facial pigmented spots are a common skin aging feature, but genetic predisposition has yet to be thoroughly investigated. We conducted a genome-wide association study for pigmented spots in 2,844 Dutch Europeans from the Rotterdam Study (mean age: 66.9±8.0 years; 47% male). Using semi-automated image analysis of high-resolution digital facial photographs, facial pigmented spots were quantified as the percentage of affected skin area (mean women: 2.0% ±0.9, men: 0.9% ±0.6). We identified genome-wide significant association with pigmented spots at three genetic loci: IRF4 (rs12203592, P=1.8 × 10(-27)), MC1R (compound heterozygosity score, P=2.3 × 10(-24)), and RALY/ASIP (rs6059655, P=1.9 × 10(-9)). In addition, after adjustment for the other three top-associated loci the BNC2 locus demonstrated significant association (rs62543565, P=2.3 × 10(-8)). The association signals observed at all four loci were successfully replicated (P<0.05) in an independent Dutch cohort (Leiden Longevity Study n=599). Although the four genes have previously been associated with skin color variation and skin cancer risk, all association signals remained highly significant (P<2 × 10(-8)) when conditioning the association analyses on skin color. We conclude that genetic variations in IRF4, MC1R, RALY/ASIP, and BNC2 contribute to the acquired amount of facial pigmented spots during aging, through pathways independent of the basal melanin production.

Cryogenic vacuumm RF feedthrough device

DOEpatents

Wu, Genfa [Yorktown, VA; Phillips, Harry Lawrence [Hayes, VA

2008-12-30

A cryogenic vacuum rf feedthrough device comprising: 1) a probe for insertion into a particle beam; 2) a coaxial cable comprising an inner conductor and an outer conductor, a dielectric/insulating layer surrounding the inner conductor, the latter being connected to the probe for the transmission of higher mode rf energy from the probe; and 3) a high thermal conductivity stub attached to the coaxial dielectric about and in thermal contact with the inner conductor which high thermal conductivity stub transmits heat generated in the vicinity of the probe efficiently and radially from the area of the probe and inner conductor all while maintaining useful rf transmission line characteristics between the inner and outer coaxial conductors.

KSC-08pd0005

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, Bob Arp, an aerospace technician with the United Launch Alliance, inserts a wire from an electrical harness onto the pin of a replacement feed-through connector during preparations to solder the pins to the socket of the connector. The connector will be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. The technician performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994 and was specifically chosen for the task. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

KSC-08pd0002

NASA Image and Video Library

2008-01-04

KENNEDY SPACE CENTER, FLA. -- At a lab at NASA's Kennedy Space Center, Kevin Wyckoff, an aerospace technician with the United Launch Alliance, examines an electrical wiring harness. The harness will be inserted into a replacement feed-through connector during preparations to solder the pins to the socket of the connector. The connector will later be installed in the external fuel tank for space shuttle Atlantis' STS-122 mission. The technician performed this exacting task on the Centaur upper stage for Atlas and Titan launches in 1994 and was specifically chosen for the task. Soldering the connector pins and sockets together addresses the most likely cause of a problem in the engine cutoff sensor system, or ECO system. Some of the tank's ECO sensors failed during propellant tanking for launch attempts on Dec. 6 and Dec. 9. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The feed-through connector passes the wires from the inside of the tank to the outside. After the soldering is completed and the connector is reinstalled, shuttle program managers will decide on how to proceed. The launch date for mission STS-122 is under review. Photo credit: NASA/Kim Shiflett

The T-plasty: a modified YV-plasty for highly recurrent bladder neck contracture after transurethral surgery for benign hyperplasia of the prostate: clinical outcome and patient satisfaction.

PubMed

Reiss, C P; Rosenbaum, C M; Becker, A; Schriefer, P; Ludwig, T A; Engel, O; Riechardt, S; Fisch, M; Dahlem, R

2016-10-01

To describe a modified surgical technique for treatment of highly recurrent bladder neck contracture (BNC) after transurethral surgery for benign hyperplasia and to evaluate success rate and patient satisfaction of this novel technique. Ten patients with highly recurrent BNC and multiple prior attempts of endoscopic treatment underwent the T-plasty. Perioperative complications were recorded and classified according to the Clavien classification. Patient reported functional outcomes were retrospectively analysed using a standardized questionnaire assessing recurrence of stenosis, incontinence, satisfaction and changes in quality of life (QoL). The questionnaires included validated IPSS and SF-8-health survey items. Mean age at the time of surgery was 69.2 years (range 61-79), and the mean follow-up was 26 months (range 3-46). No complications grade 3 or higher according to the Clavien classification occurred. Success rate was 100 %. No de novo stress incontinence occurred. Urinary stream was described as very strong to moderate by 80 % of the patients, mean post-operative IPSS-score was 11.3 (range 4-29), and mean post-operative IPSS-QoL was 2.4 (range 1-5). Patients satisfaction was very high or high in 90 %, and QoL improved in 90 %. The SF-8-health survey showed values comparable to the reference population. The T-plasty represents a safe and valuable option in treating highly recurrent BNC after surgery for benign hyperplasia. It offers multiple advantages compared to other techniques such as a single-staged approach and the opportunity for reconstruction of a reliable wide bladder neck by usage of two well-vascularized flaps. Success rate, low rate of complications and preservation of continence are highly encouraging.

A Modularity-Based Method Reveals Mixed Modules from Chemical-Gene Heterogeneous Network

PubMed Central

Song, Jianglong; Tang, Shihuan; Liu, Xi; Gao, Yibo; Yang, Hongjun; Lu, Peng

2015-01-01

For a multicomponent therapy, molecular network is essential to uncover its specific mode of action from a holistic perspective. The molecular system of a Traditional Chinese Medicine (TCM) formula can be represented by a 2-class heterogeneous network (2-HN), which typically includes chemical similarities, chemical-target interactions and gene interactions. An important premise of uncovering the molecular mechanism is to identify mixed modules from complex chemical-gene heterogeneous network of a TCM formula. We thus proposed a novel method (MixMod) based on mixed modularity to detect accurate mixed modules from 2-HNs. At first, we compared MixMod with Clauset-Newman-Moore algorithm (CNM), Markov Cluster algorithm (MCL), Infomap and Louvain on benchmark 2-HNs with known module structure. Results showed that MixMod was superior to other methods when 2-HNs had promiscuous module structure. Then these methods were tested on a real drug-target network, in which 88 disease clusters were regarded as real modules. MixMod could identify the most accurate mixed modules from the drug-target 2-HN (normalized mutual information 0.62 and classification accuracy 0.4524). In the end, MixMod was applied to the 2-HN of Buchang naoxintong capsule (BNC) and detected 49 mixed modules. By using enrichment analysis, we investigated five mixed modules that contained primary constituents of BNC intestinal absorption liquid. As a matter of fact, the findings of in vitro experiments using BNC intestinal absorption liquid were found to highly accord with previous analysis. Therefore, MixMod is an effective method to detect accurate mixed modules from chemical-gene heterogeneous networks and further uncover the molecular mechanism of multicomponent therapies, especially TCM formulae. PMID:25927435

Comparing the effects of two Swedish massage techniques on the vital signs and anxiety of healthy women

PubMed Central

Gholami-Motlagh, Farzaneh; Jouzi, Mina; Soleymani, Bahram

2016-01-01

Background: Anxiety is an inseparable part of our lives and a serious threat to health. Therefore, it is necessary to use certain strategies to prevent disorders caused by anxiety and adjust the vital signs of people. Swedish massage is one of the most recognized techniques for reducing anxiety. This study aims to compare the effects of two massage techniques on the vital signs and anxiety of healthy women. Materials and Methods: This quasi-experimental study with a two-group, crossover design was conducted on 20 healthy women who were selected by simple sampling method and were randomly assigned to BNC (Back, Neck, and Chest) or LAF (Leg, Arm, and Face) groups. Massage therapy was carried out for a 14-week period (two 4-week massage therapy sessions and 6 weeks washout stage). Gathered data were analyzed using paired t-test with a significance level of P < 0.05. Results: Both BNC and LAF methods caused a significant decrease in systolic BP in the first stage (P = 0.02, 0.00); however, diastolic BP showed significant decrease only in BNC group (P = 0.01). The mean average of body temperature of LAF group showed a significant decrease in the first stage (P = 0.0.3), and pulse and respiratory rate showed significant decrease in both groups during the second stage (P = 0.00). In addition, anxiety scores showed no significant difference before and after massage therapy (P < 0.05). Conclusions: Massage therapy caused a decrease in systolic BP, pulse, and respiratory rate. It can be concluded that massage therapy was useful for decreasing the vital signs associated with anxiety in healthy women. PMID:27563325

The Synthesis, Characterization and Dehydrogenation of Sigma-Complexes of BN-Cyclohexanes

DOE PAGES

Kumar, Amit; Ishibashi, Jacob S. A.; Hooper, Thomas N.; ...

2015-11-25

The coordination chemistry of the 1,2-BN-cyclohexanes 2,2-R 2-1,2-B,N-C 4H 10 (R 2=HH, MeH, Me 2) with Ir and Rh metal fragments has been studied. This led to the solution (NMR spectroscopy) and solid-state (X-ray diffraction) characterization of [Ir(PCy 3) 2(H) 2(η 2η 2-H 2BNR 2C 4H 8)][BAr F 4] (NR 2=NH 2, NMeH) and [Rh( iPr 2PCH 2CH 2CH 2P iPr 2)(η 2η 2-H 2BNR 2C 4H 8)][BAr F 4] (NR 2=NH 2, NMeH, NMe 2). For NR 2=NH 2 subsequent metal-promoted, dehydrocoupling shows the eventual formation of the cyclic tricyclic borazine [BNC 4H 8] 3, via amino-borane and, tentativelymore » characterized using DFT/GIAO chemical shift calculations, cycloborazane intermediates. For NR 2=NMeH the final product is the cyclic amino-borane HBNMeC 4H 8. The mechanism of dehydrogenation of 2,2-H,Me-1,2-B,N-C 4H 10 using the {Rh( iPr 2PCH 2CH 2CH 2P iPr 2)} + catalyst has been probed. Catalytic experiments indicate the rapid formation of a dimeric species, [Rh 2( iPr 2PCH 2CH 2CH 2P iPr 2) 2H 5][BAr F 4]. Using the initial rate method starting from this dimer, a first-order relationship to [amine-borane], but half-order to [Rh] is established, which is suggested to be due to a rapid dimer–monomer equilibrium operating.« less

The brain as a "hyper-network": the key role of neural networks as main producers of the integrated brain actions especially via the "broadcasted" neuroconnectomics.

PubMed

Agnati, Luigi F; Marcoli, Manuela; Maura, Guido; Woods, Amina; Guidolin, Diego

2018-06-01

Investigations of brain complex integrative actions should consider beside neural networks, glial, extracellular molecular, and fluid channels networks. The present paper proposes that all these networks are assembled into the brain hyper-network that has as fundamental components, the tetra-partite synapses, formed by neural, glial, and extracellular molecular networks. Furthermore, peri-synaptic astrocytic processes by modulating the perviousness of extracellular fluid channels control the signals impinging on the tetra-partite synapses. It has also been surmised that global signalling via astrocytes networks and highly pervasive signals, such as electromagnetic fields (EMFs), allow the appropriate integration of the various networks especially at crucial nodes level, the tetra-partite synapses. As a matter of fact, it has been shown that astrocytes can form gap-junction-coupled syncytia allowing intercellular communication characterised by a rapid and possibly long-distance transfer of signals. As far as the EMFs are concerned, the concept of broadcasted neuroconnectomics (BNC) has been introduced to describe highly pervasive signals involved in resetting the information handling of brain networks at various miniaturisation levels. In other words, BNC creates, thanks to the EMFs, generated especially by neurons, different assemblages among the various networks forming the brain hyper-network. Thus, it is surmised that neuronal networks are the "core components" of the brain hyper-network that has as special "nodes" the multi-facet tetra-partite synapses. Furthermore, it is suggested that investigations on the functional plasticity of multi-partite synapses in response to BNC can be the background for a new understanding and perhaps a new modelling of brain morpho-functional organisation and integrative actions.

Programmable bio-nano-chip systems for serum CA125 quantification: toward ovarian cancer diagnostics at the point-of-care.

PubMed

Raamanathan, Archana; Simmons, Glennon W; Christodoulides, Nicolaos; Floriano, Pierre N; Furmaga, Wieslaw B; Redding, Spencer W; Lu, Karen H; Bast, Robert C; McDevitt, John T

2012-05-01

Point-of-care (POC) implementation of early detection and screening methodologies for ovarian cancer may enable improved survival rates through early intervention. Current laboratory-confined immunoanalyzers have long turnaround times and are often incompatible with multiplexing and POC implementation. Rapid, sensitive, and multiplexable POC diagnostic platforms compatible with promising early detection approaches for ovarian cancer are needed. To this end, we report the adaptation of the programmable bio-nano-chip (p-BNC), an integrated, microfluidic, and modular (programmable) platform for CA125 serum quantitation, a biomarker prominently implicated in multimodal and multimarker screening approaches. In the p-BNCs, CA125 from diseased sera (Bio) is sequestered and assessed with a fluorescence-based sandwich immunoassay, completed in the nano-nets (Nano) of sensitized agarose microbeads localized in individually addressable wells (Chip), housed in a microfluidic module, capable of integrating multiple sample, reagent and biowaste processing, and handling steps. Antibody pairs that bind to distinct epitopes on CA125 were screened. To permit efficient biomarker sequestration in a three-dimensional microfluidic environment, the p-BNC operating variables (incubation times, flow rates, and reagent concentrations) were tuned to deliver optimal analytical performance under 45 minutes. With short analysis times, competitive analytical performance (inter- and intra-assay precision of 1.2% and 1.9% and limit of detection of 1.0 U/mL) was achieved on this minisensor ensemble. Furthermore, validation with sera of patients with ovarian cancer (n = 20) showed excellent correlation (R(2) = 0.97) with gold-standard ELISA. Building on the integration capabilities of novel microfluidic systems programmed for ovarian cancer, the rapid, precise, and sensitive miniaturized p-BNC system shows strong promise for ovarian cancer diagnostics.

Feedback-Resistant Acetohydroxy Acid Synthase Increases Valine Production in Corynebacterium glutamicum

PubMed Central

Elišáková, Veronika; Pátek, Miroslav; Holátko, Jiří; Nešvera, Jan; Leyval, Damien; Goergen, Jean-Louis; Delaunay, Stéphane

2005-01-01

Acetohydroxy acid synthase (AHAS), which catalyzes the key reactions in the biosynthesis pathways of branched-chain amino acids (valine, isoleucine, and leucine), is regulated by the end products of these pathways. The whole Corynebacterium glutamicum ilvBNC operon, coding for acetohydroxy acid synthase (ilvBN) and aceto hydroxy acid isomeroreductase (ilvC), was cloned in the newly constructed Escherichia coli-C. glutamicum shuttle vector pECKA (5.4 kb, Kmr). By using site-directed mutagenesis, one to three amino acid alterations (mutations M8, M11, and M13) were introduced into the small (regulatory) AHAS subunit encoded by ilvN. The activity of AHAS and its inhibition by valine, isoleucine, and leucine were measured in strains carrying the ilvBNC operon with mutations on the plasmid or the ilvNM13 mutation within the chromosome. The enzyme containing the M13 mutation was feedback resistant to all three amino acids. Different combinations of branched-chain amino acids did not inhibit wild-type AHAS to a greater extent than was measured in the presence of 5 mM valine alone (about 57%). We infer from these results that there is a single binding (allosteric) site for all three amino acids in the enzyme molecule. The strains carrying the ilvNM13 mutation in the chromosome produced more valine than their wild-type counterparts. The plasmid-free C. glutamicum ΔilvA ΔpanB ilvNM13 strain formed 90 mM valine within 48 h of cultivation in minimal medium. The same strain harboring the plasmid pECKAilvBNC produced as much as 130 mM valine under the same conditions. PMID:15640189

KSC-08pd0072

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, a technician begins attaching the cover over the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0073

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, a technician attaches the cover over the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0069

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, technicians prepare the cover to be installed over the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0074

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, a technician completes installing the cover over the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

KSC-08pd0071

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, a technician gets ready to place the cover over the engine cutoff, or ECO, sensor system connector and wiring on space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

Tunable thermal rectification in graphene/hexagonal boron nitride hybrid structures

NASA Astrophysics Data System (ADS)

Chen, Xue-Kun; Hu, Ji-Wen; Wu, Xi-Jun; Jia, Peng; Peng, Zhi-Hua; Chen, Ke-Qiu

2018-02-01

Using non-equilibrium molecular dynamics simulations, we investigate thermal rectification (TR) in graphene/hexagonal boron nitride (h-BN) hybrid structures. Two different structural models, partially substituting graphene into h-BN (CBN) and partially substituting h-BN into graphene (BNC), are considered. It is found that CBN has a significant TR effect while that of BNC is very weak. The observed TR phenomenon can be attributed to the resonance effect between out-of-plane phonons of graphene and h-BN domains in the low-frequency region under negative temperature bias. In addition, the influences of ambient temperature, system size, defect number and substrate interaction are also studied to obtain the optimum conditions for TR. More importantly, the TR ratio could be effectively tuned through chemical and structural diversity. A moderate C/BN ratio and parallel arrangement are found to enhance the TR ratio. Detailed phonon spectra analyses are conducted to understand the thermal transport behavior. This work extends hybrid engineering to 2D materials for achieving TR.

Programmable Pulse Generator for Aditya Gas Puffing System

NASA Astrophysics Data System (ADS)

Patel, Narendra; Chavda, Chhaya; Bhatt, S. B.; Chattopadhyay, Prabal; Saxena, Y. C.

2012-11-01

In the Aditya Tokamak, one of primary requirement for plasma generation is to feed the required quantity of the fuel gas prior to plasma shot. Gas feed system mainly consists of piezoelectric gas leak valve and gas reservoir. The Hydrogen gas is prior to 300ms loop voltage for the duration of 4 msec to 7 msec. Gas is puffed during the shot for required plasma parameters and to increase plasma density using the same system. The valve is controlled by either continuous voltage or pulses of different width, amplitude and delay with respect to loop voltage. These voltage pulses are normally applied through standard pulse generator. The standard pulse generator is replaced by micro controller based in housed developed programmable pulse generator system consists of in built power supply, BNC input for external trigger, BNC output and serial interface. This programmable pulse generator is successfully tested and is in operation for gas puffing during ADITYA Tokamak experiments. The paper discusses the design and development aspect of the system.

Dimensions and Measurements of Debuncher Band 3 and 4 Waveguide-Coax Launchers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, Ding; /Fermilab

2000-09-13

This note is a document about dimensions and measurement results of waveguide-coax launchers (Band 3 and 4) installed on the arrays in debuncher cooling upgrade. Shown in Figure 1 and 5 are schematic drawings of launchers (pick-up) in the cross section along the longitudinal direction (beam direction) of the arrays. The unit in these drawings is inch. Note: although there are upper band and lower band for pickup arrays, the launchers are the same to avoid possible confusion during installation. Launchers for band 3 and 4 kickers were made by Penn-engineering Inc., therefor no schematic drawings are presented in thismore » note. RF Measurements were made on all launchers (port) and printed in hard copies for future reference. Since the measurement results are similar to each other, only a few plots for each type of launcher/band are presented in this document. There are two types of measured S11 parameters. One is the measurement made at the end of design/tuning stage using a launcher and a straight section of band 3 or 4 waveguide terminated with a cone of absorber. I use 'Original' to denote this kind of measurement. As shown in Figure 2, 6, 9 and 12, the original S11 of all launchers are below or around -20 db over the full band 3 or 4. The other type of measurement is the one made after these launchers were installed onto the array including several type N feedthrough or connectors, elbows, waveguide bends (kicker) and magic Ts (kicker) etc. The kicker arrays were terminated with wedges of absorber. During all measurements (pickup array or kicker array) when one launcher was being measured, all other launchers were terminated with 50 ohm terminator. As shown in Figure 3, 4, 7, 8, 10, 11, 13 and 14 these 'final' S11s are around -15 db.« less

Map of Pseudo-F-statistics of seismic noise parameters as an indicator of current seismic danger in Japan

NASA Astrophysics Data System (ADS)

Lyubushin, Alexey

2016-04-01

The problem of estimate of current seismic danger based on monitoring of seismic noise properties from broadband seismic network F-net in Japan (84 stations) is considered. Variations of the following seismic noise parameters are analyzed: multifractal singularity spectrum support width, generalized Hurst exponent, minimum Hölder-Lipschitz exponent and minimum normalized entropy of squared orthogonal wavelet coefficients. These parameters are estimated within adjacent time windows of the length 1 day for seismic noise waveforms from each station. Calculating daily median values of these parameters by all stations provides 4-dimensional time series which describes integral properties of the seismic noise in the region covered by the network. Cluster analysis is applied to the sequence of clouds of 4-dimensional vectors within moving time window of the length 365 days with mutual shift 3 days starting from the beginning of 1997 up to the current time. The purpose of the cluster analysis is to find the best number of clusters (BNC) from probe numbers which are varying from 1 up to the maximum value 40. The BNC is found from the maximum of pseudo-F-statistics (PFS). A 2D map could be created which presents dependence of PFS on the tested probe number of clusters and the right-hand end of moving time window which is rather similar to usual spectral time-frequency diagrams. In the paper [1] it was shown that the BNC before Tohoku mega-earthquake on March 11, 2011, has strongly chaotic regime with jumps from minimum up to maximum values in the time interval 1 year before the event and this time intervals was characterized by high PFS values. The PFS-map is proposed as the method for extracting time intervals with high current seismic danger. The next danger time interval after Tohoku mega-EQ began at the end of 2012 and was finished at the middle of 2013. Starting from middle of 2015 the high PFS values and chaotic regime of BNC variations were returned. This could be interpreted as the increasing of the danger of the next mega-EQ in Japan in the region of Nankai Trough [1] at the first half of 2016. References 1. Lyubushin, A., 2013. How soon would the next mega-earthquake occur in Japan? // Natural Science, 5 (8A1), 1-7. http://dx.doi.org/10.4236/ns.2013.58A1001

Optical readout of a two phase liquid argon TPC using CCD camera and THGEMs

NASA Astrophysics Data System (ADS)

Mavrokoridis, K.; Ball, F.; Carroll, J.; Lazos, M.; McCormick, K. J.; Smith, N. A.; Touramanis, C.; Walker, J.

2014-02-01

This paper presents a preliminary study into the use of CCDs to image secondary scintillation light generated by THick Gas Electron Multipliers (THGEMs) in a two phase LAr TPC. A Sony ICX285AL CCD chip was mounted above a double THGEM in the gas phase of a 40 litre two-phase LAr TPC with the majority of the camera electronics positioned externally via a feedthrough. An Am-241 source was mounted on a rotatable motion feedthrough allowing the positioning of the alpha source either inside or outside of the field cage. Developed for and incorporated into the TPC design was a novel high voltage feedthrough featuring LAr insulation. Furthermore, a range of webcams were tested for operation in cryogenics as an internal detector monitoring tool. Of the range of webcams tested the Microsoft HD-3000 (model no:1456) webcam was found to be superior in terms of noise and lowest operating temperature. In ambient temperature and atmospheric pressure 1 ppm pure argon gas, the THGEM gain was ≈ 1000 and using a 1 msec exposure the CCD captured single alpha tracks. Successful operation of the CCD camera in two-phase cryogenic mode was also achieved. Using a 10 sec exposure a photograph of secondary scintillation light induced by the Am-241 source in LAr has been captured for the first time.

Investigation on the hermeticity of an implantable package with 32 feedthroughs for neural prosthetic applications.

PubMed

Bisoni, Lorenzo; Mueller, Matthias; Cvancara, Paul; Carboni, Caterina; Puddu, Roberto; Raffo, Luigi; Barbaro, Massimo; Stieglitz, Thomas

2016-08-01

This paper presents an implantable package aimed at hosting a bidirectional neural interface for neural prosthetic applications. The package has been conceived to minimize the invasivity for the patient, for this reason a cylindrical container with an outer diameter of 7 mm and a length of 21 mm has been designed. The package, realized in alumina (Al2O3), presents 32 hermetic feedthroughs located at the top and bottom base of the cylinder. The hermetic housing has been assembled using a low-temperature soldering method based on a previous platinum/gold (Pt/Au) metallization of the ceramic parts. The package's hermeticity has been successfully proved by means of in-vitro tests, exhibiting an increase in the inner relative humidity of 20 %RH over 75 days of observation.

High voltage feedthrough bushing

DOEpatents

Brucker, John P.

1993-01-01

A feedthrough bushing for a high voltage diode provides for using compression sealing for all sealing surfaces. A diode assembly includes a central conductor extending through the bushing and a grading ring assembly circumferentially surrounding and coaxial with the central conductor. A flexible conductive plate extends between and compressively seals against the central conductor and the grading ring assembly, wherein the flexibility of the plate allows inner and outer portions of the plate to axially translate for compression sealing against the central conductor and the grading ring assembly, respectively. The inner portion of the plate is bolted to the central conductor for affecting sealing. A compression beam is also bolted to the central conductor and engages the outer portion of the plate to urge the outer portion toward the grading ring assembly to obtain compression sealing therebetween.

KSC-08pd0070

NASA Image and Video Library

2008-01-17

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A at NASA's Kennedy Space Center, technicians prepare the cover to be installed over the engine cutoff, or ECO, sensor system connector and wiring on space shuttle space shuttle Atlantis' external tank. The feed-through connector passes the wires from the inside of the tank to the outside. Results of a tanking test on Dec. 18 pointed to an open circuit in the feed-through connector wiring, which is located at the base of the tank. The pins in the replacement connector were precisely soldered to create a connection that allows sensors inside the tank to send signals to the computers onboard Atlantis. The launch date for the shuttle's STS-122 mission has now been targeted for Feb. 7. Photo credit: NASA/Kim Shiflett

Broadband nanoindentation of glassy polymers: Part II. Viscoplasticity

Treesearch

Joseph E. Jakes; Rod S. Lakes; Don S. Stone

2012-01-01

The relationship between hardness and flow stress in glassy polymers is examined. Materials studied include poly(methylmethacrylate), polystyrene, and polycarbonate. Properties are strongly rate dependent, so broadband nanoindentation creep (BNC) is used to measure hardness across a broad range of indentation strain rates (10-4 to 10 s

Glycosylation and immunocytochemistry of binucleate cells in pronghorn (Antilocapra Americana, Antilocapridae) shows features of both Giraffidae and Bovidae

USDA-ARS?s Scientific Manuscript database

Although the Pronghorn (Antilocapra americana) resembles an antelope, its nearest relatives are the Giraffe and Okapi. In this study we have examined the placentae of 6 pronghorns using lectin histochemistry to identify any giraffid features. Results showed that the binucleate cell (BNC) of the pla...

A Corpus-Based Comparative Study of "Learn" and "Acquire"

ERIC Educational Resources Information Center

Yang, Bei

2016-01-01

As an important yet intricate linguistic feature in English language, synonymy poses a great challenge for second language learners. Using the 100 million-word British National Corpus (BNC) as data and the software Sketch Engine (SkE) as an analyzing tool, this article compares the usage of "learn" and "acquire" used in natural…

Carbon-tuned bonding method significantly enhanced the hydrogen storage of BN-Li complexes.

PubMed

Deng, Qing-ming; Zhao, Lina; Luo, You-hua; Zhang, Meng; Zhao, Li-xia; Zhao, Yuliang

2011-11-01

Through first-principles calculations, we found doping carbon atoms onto BN monolayers (BNC) could significantly strengthen the Li bond on this material. Unlike the weak bond strength between Li atoms and the pristine BN layer, it is observed that Li atoms are strongly hybridized and donate their electrons to the doped substrate, which is responsible for the enhanced binding energy. Li adsorbed on the BNC layer can serve as a high-capacity hydrogen storage medium, without forming clusters, which can be recycled at room temperature. Eight polarized H(2) molecules are attached to two Li atoms with an optimal binding energy of 0.16-0.28 eV/H(2), which results from the electrostatic interaction of the polarized charge of hydrogen molecules with the electric field induced by positive Li atoms. This practical carbon-tuned BN-Li complex can work as a very high-capacity hydrogen storage medium with a gravimetric density of hydrogen of 12.2 wt%, which is much higher than the gravimetric goal of 5.5 wt % hydrogen set by the U.S. Department of Energy for 2015.

Bus bar electrical feedthrough for electrorefiner system

DOEpatents

Williamson, Mark; Wiedmeyer, Stanley G; Willit, James L; Barnes, Laurel A; Blaskovitz, Robert J

2013-12-03

A bus bar electrical feedthrough for an electrorefiner system may include a retaining plate, electrical isolator, and/or contact block. The retaining plate may include a central opening. The electrical isolator may include a top portion, a base portion, and a slot extending through the top and base portions. The top portion of the electrical isolator may be configured to extend through the central opening of the retaining plate. The contact block may include an upper section, a lower section, and a ridge separating the upper and lower sections. The upper section of the contact block may be configured to extend through the slot of the electrical isolator and the central opening of the retaining plate. Accordingly, relatively high electrical currents may be transferred into a glovebox or hot-cell facility at a relatively low cost and higher amperage capacity without sacrificing atmosphere integrity.

Performance degradation of ferrofluidic feedthroughs in a mixed irradiation field

NASA Astrophysics Data System (ADS)

Simos, Nikolaos; Fernandes, S.; Mittig, Wolfgang; Pellemoine, Frederique; Avilov, M.; Kostin, M.; Mausner, L.; Ronningen, R.; Schein, M.; Bollen, G.

2017-01-01

Ferrofluidic feedthrough (FF) rotary seals containing either NdFeB or SmCo-type permanent magnets have been considered for use in the target and beam dump systems of the Facility for Rare Isotope Beams (FRIB). To evaluate their performance under irradiation three FF seals were irradiated in a mixed field consisting of fast neutrons, protons and γ-rays to an average absorbed dose of 0.2, 2.0, and 20.0 MGy at the Brookhaven Linac Isotope Producer facility (BLIP). The radiation types and energy profiles mimic those expected at the FRIB facility. Degradation of the operational performance of these devices due to irradiation is expected to be the result of the de-magnetization of the permanent magnets contained within the seal and the changes in the ferrofluid properties. Post-irradiation performance was evaluated by determining the ferrofluidic seal vacuum tightness and torque under static and dynamic conditions. The study revealed that the ferrofluidic feedthrough seal irradiated to a dose of 0.2 MGy maintained its vacuum tightness under both static and rotational condition while the one irradiated to a dose of 2.0 MGy exhibited signs of ferrofluid damage but no overall performance loss. At 20 MGy dose the effects of irradiation on the ferrofluid properties (viscosity and particle agglomeration) were shown to be severe. Furthermore, limited de-magnetization of the annular shaped Nd2Fe14B and Sm2Co17 magnets located within the irradiated FFs was observed for doses of 0.2 MGy and 20 MGy respectively.

Fine-Scale Mapping at 9p22.2 Identifies Candidate Causal Variants That Modify Ovarian Cancer Risk in BRCA1 and BRCA2 Mutation Carriers

PubMed Central

Vigorito, Elena; Kuchenbaecker, Karoline B.; Beesley, Jonathan; Adlard, Julian; Agnarsson, Bjarni A.; Andrulis, Irene L.; Arun, Banu K.; Barjhoux, Laure; Belotti, Muriel; Benitez, Javier; Berger, Andreas; Bojesen, Anders; Bonanni, Bernardo; Brewer, Carole; Caldes, Trinidad; Caligo, Maria A.; Campbell, Ian; Chan, Salina B.; Claes, Kathleen B. M.; Cohn, David E.; Cook, Jackie; Daly, Mary B.; Damiola, Francesca; Davidson, Rosemarie; de Pauw, Antoine; Delnatte, Capucine; Diez, Orland; Domchek, Susan M.; Dumont, Martine; Durda, Katarzyna; Dworniczak, Bernd; Easton, Douglas F.; Eccles, Diana; Edwinsdotter Ardnor, Christina; Eeles, Ros; Ejlertsen, Bent; Ellis, Steve; Evans, D. Gareth; Feliubadalo, Lidia; Fostira, Florentia; Foulkes, William D.; Friedman, Eitan; Frost, Debra; Gaddam, Pragna; Ganz, Patricia A.; Garber, Judy; Garcia-Barberan, Vanesa; Gauthier-Villars, Marion; Gehrig, Andrea; Gerdes, Anne-Marie; Giraud, Sophie; Godwin, Andrew K.; Goldgar, David E.; Hake, Christopher R.; Hansen, Thomas V. O.; Healey, Sue; Hodgson, Shirley; Hogervorst, Frans B. L.; Houdayer, Claude; Hulick, Peter J.; Imyanitov, Evgeny N.; Isaacs, Claudine; Izatt, Louise; Izquierdo, Angel; Jacobs, Lauren; Jakubowska, Anna; Janavicius, Ramunas; Jaworska-Bieniek, Katarzyna; Jensen, Uffe Birk; John, Esther M.; Vijai, Joseph; Karlan, Beth Y.; Kast, Karin; Investigators, KConFab; Khan, Sofia; Kwong, Ava; Laitman, Yael; Lester, Jenny; Lesueur, Fabienne; Liljegren, Annelie; Lubinski, Jan; Mai, Phuong L.; Manoukian, Siranoush; Mazoyer, Sylvie; Meindl, Alfons; Mensenkamp, Arjen R.; Montagna, Marco; Nathanson, Katherine L.; Neuhausen, Susan L.; Nevanlinna, Heli; Niederacher, Dieter; Olah, Edith; Olopade, Olufunmilayo I.; Ong, Kai-ren; Osorio, Ana; Park, Sue Kyung; Paulsson-Karlsson, Ylva; Pedersen, Inge Sokilde; Peissel, Bernard; Peterlongo, Paolo; Pfeiler, Georg; Phelan, Catherine M.; Piedmonte, Marion; Poppe, Bruce; Pujana, Miquel Angel; Radice, Paolo; Rennert, Gad; Rodriguez, Gustavo C.; Rookus, Matti A.; Ross, Eric A.; Schmutzler, Rita Katharina; Simard, Jacques; Singer, Christian F.; Slavin, Thomas P.; Soucy, Penny; Southey, Melissa; Steinemann, Doris; Stoppa-Lyonnet, Dominique; Sukiennicki, Grzegorz; Sutter, Christian; Szabo, Csilla I.; Tea, Muy-Kheng; Teixeira, Manuel R.; Teo, Soo-Hwang; Terry, Mary Beth; Thomassen, Mads; Tibiletti, Maria Grazia; Tihomirova, Laima; Tognazzo, Silvia; van Rensburg, Elizabeth J.; Varesco, Liliana; Varon-Mateeva, Raymonda; Vratimos, Athanassios; Weitzel, Jeffrey N.; McGuffog, Lesley; Kirk, Judy; Toland, Amanda Ewart; Hamann, Ute; Lindor, Noralane; Ramus, Susan J.; Greene, Mark H.; Couch, Fergus J.; Offit, Kenneth; Pharoah, Paul D. P.; Chenevix-Trench, Georgia; Antoniou, Antonis C.

2016-01-01

Population-based genome wide association studies have identified a locus at 9p22.2 associated with ovarian cancer risk, which also modifies ovarian cancer risk in BRCA1 and BRCA2 mutation carriers. We conducted fine-scale mapping at 9p22.2 to identify potential causal variants in BRCA1 and BRCA2 mutation carriers. Genotype data were available for 15,252 (2,462 ovarian cancer cases) BRCA1 and 8,211 (631 ovarian cancer cases) BRCA2 mutation carriers. Following genotype imputation, ovarian cancer associations were assessed for 4,873 and 5,020 SNPs in BRCA1 and BRCA 2 mutation carriers respectively, within a retrospective cohort analytical framework. In BRCA1 mutation carriers one set of eight correlated candidate causal variants for ovarian cancer risk modification was identified (top SNP rs10124837, HR: 0.73, 95%CI: 0.68 to 0.79, p-value 2× 10−16). These variants were located up to 20 kb upstream of BNC2. In BRCA2 mutation carriers one region, up to 45 kb upstream of BNC2, and containing 100 correlated SNPs was identified as candidate causal (top SNP rs62543585, HR: 0.69, 95%CI: 0.59 to 0.80, p-value 1.0 × 10−6). The candidate causal in BRCA1 mutation carriers did not include the strongest associated variant at this locus in the general population. In sum, we identified a set of candidate causal variants in a region that encompasses the BNC2 transcription start site. The ovarian cancer association at 9p22.2 may be mediated by different variants in BRCA1 mutation carriers and in the general population. Thus, potentially different mechanisms may underlie ovarian cancer risk for mutation carriers and the general population. PMID:27463617

Hapten Optimization for Cocaine Vaccine with Improved Cocaine Recognition

PubMed Central

Ramakrishnan, Muthu; Kinsey, Berma M.; Singh, Rana A.; Kosten, Thomas R.; Orson, Frank M.

2014-01-01

In the absence of any effective pharmacotherapy for cocaine addiction, immunotherapy is being actively pursued as a therapeutic intervention. While several different cocaine haptens have been explored to develop anti-cocaine antibodies, none of the hapten was successfully designed which had a protonated tropane nitrogen as is found in native cocaine under physiological conditions, including the succinyl norcocaine (SNC) hapten that has been tested in phase II clinical trials. Herein, we discuss three different cocaine haptens: hexyl-norcocaine (HNC), bromoacetamido butyl- norcocaine (BNC), and succinyl-butyl- norcocaine (SBNC), each with a tertiary nitrogen structure mimicking that of native cocaine which could optimize the specificity of anti-cocaine antibodies for better cocaine recognition. Mice immunized with these haptens conjugated to immunogenic proteins produced high titer anti-cocaine antibodies. However, during chemical conjugation of HNC and BNC haptens to carrier proteins, the 2β methyl ester group is hydrolyzed and immunizing mice with these conjugate vaccines in mice produced antibodies that bound both cocaine and the inactive benzoylecgonine metabolite. While in the case of the SBNC conjugate vaccine hydrolysis of the methyl ester did not appear to occur, leading to antibodies with high specificity to cocaine over BE. Though we observed similar specificity with a SNC hapten, the striking difference is that SBNC carries a positive charge on the tropane nitrogen atom, and therefore it is expected to have better binding of cocaine. The 50% cocaine inhibitory concentration (IC50) value for SBNC antibodies (2.8 μM) was significantly better than the SNC antibodies (9.4 μM) when respective hapten-BSA was used as a substrate. In addition, antibodies from both sera had no inhibitory effect from BE. In contrast to BNC and HNC, the SBNC conjugate was also found to be highly stable without any noticeable hydrolysis for several months at 4°C and 2-3 days in pH 10 buffer at 37°C. PMID:24803171

Fine-Scale Mapping at 9p22.2 Identifies Candidate Causal Variants That Modify Ovarian Cancer Risk in BRCA1 and BRCA2 Mutation Carriers.

PubMed

Vigorito, Elena; Kuchenbaecker, Karoline B; Beesley, Jonathan; Adlard, Julian; Agnarsson, Bjarni A; Andrulis, Irene L; Arun, Banu K; Barjhoux, Laure; Belotti, Muriel; Benitez, Javier; Berger, Andreas; Bojesen, Anders; Bonanni, Bernardo; Brewer, Carole; Caldes, Trinidad; Caligo, Maria A; Campbell, Ian; Chan, Salina B; Claes, Kathleen B M; Cohn, David E; Cook, Jackie; Daly, Mary B; Damiola, Francesca; Davidson, Rosemarie; Pauw, Antoine de; Delnatte, Capucine; Diez, Orland; Domchek, Susan M; Dumont, Martine; Durda, Katarzyna; Dworniczak, Bernd; Easton, Douglas F; Eccles, Diana; Edwinsdotter Ardnor, Christina; Eeles, Ros; Ejlertsen, Bent; Ellis, Steve; Evans, D Gareth; Feliubadalo, Lidia; Fostira, Florentia; Foulkes, William D; Friedman, Eitan; Frost, Debra; Gaddam, Pragna; Ganz, Patricia A; Garber, Judy; Garcia-Barberan, Vanesa; Gauthier-Villars, Marion; Gehrig, Andrea; Gerdes, Anne-Marie; Giraud, Sophie; Godwin, Andrew K; Goldgar, David E; Hake, Christopher R; Hansen, Thomas V O; Healey, Sue; Hodgson, Shirley; Hogervorst, Frans B L; Houdayer, Claude; Hulick, Peter J; Imyanitov, Evgeny N; Isaacs, Claudine; Izatt, Louise; Izquierdo, Angel; Jacobs, Lauren; Jakubowska, Anna; Janavicius, Ramunas; Jaworska-Bieniek, Katarzyna; Jensen, Uffe Birk; John, Esther M; Vijai, Joseph; Karlan, Beth Y; Kast, Karin; Investigators, KConFab; Khan, Sofia; Kwong, Ava; Laitman, Yael; Lester, Jenny; Lesueur, Fabienne; Liljegren, Annelie; Lubinski, Jan; Mai, Phuong L; Manoukian, Siranoush; Mazoyer, Sylvie; Meindl, Alfons; Mensenkamp, Arjen R; Montagna, Marco; Nathanson, Katherine L; Neuhausen, Susan L; Nevanlinna, Heli; Niederacher, Dieter; Olah, Edith; Olopade, Olufunmilayo I; Ong, Kai-Ren; Osorio, Ana; Park, Sue Kyung; Paulsson-Karlsson, Ylva; Pedersen, Inge Sokilde; Peissel, Bernard; Peterlongo, Paolo; Pfeiler, Georg; Phelan, Catherine M; Piedmonte, Marion; Poppe, Bruce; Pujana, Miquel Angel; Radice, Paolo; Rennert, Gad; Rodriguez, Gustavo C; Rookus, Matti A; Ross, Eric A; Schmutzler, Rita Katharina; Simard, Jacques; Singer, Christian F; Slavin, Thomas P; Soucy, Penny; Southey, Melissa; Steinemann, Doris; Stoppa-Lyonnet, Dominique; Sukiennicki, Grzegorz; Sutter, Christian; Szabo, Csilla I; Tea, Muy-Kheng; Teixeira, Manuel R; Teo, Soo-Hwang; Terry, Mary Beth; Thomassen, Mads; Tibiletti, Maria Grazia; Tihomirova, Laima; Tognazzo, Silvia; van Rensburg, Elizabeth J; Varesco, Liliana; Varon-Mateeva, Raymonda; Vratimos, Athanassios; Weitzel, Jeffrey N; McGuffog, Lesley; Kirk, Judy; Toland, Amanda Ewart; Hamann, Ute; Lindor, Noralane; Ramus, Susan J; Greene, Mark H; Couch, Fergus J; Offit, Kenneth; Pharoah, Paul D P; Chenevix-Trench, Georgia; Antoniou, Antonis C

2016-01-01

Population-based genome wide association studies have identified a locus at 9p22.2 associated with ovarian cancer risk, which also modifies ovarian cancer risk in BRCA1 and BRCA2 mutation carriers. We conducted fine-scale mapping at 9p22.2 to identify potential causal variants in BRCA1 and BRCA2 mutation carriers. Genotype data were available for 15,252 (2,462 ovarian cancer cases) BRCA1 and 8,211 (631 ovarian cancer cases) BRCA2 mutation carriers. Following genotype imputation, ovarian cancer associations were assessed for 4,873 and 5,020 SNPs in BRCA1 and BRCA 2 mutation carriers respectively, within a retrospective cohort analytical framework. In BRCA1 mutation carriers one set of eight correlated candidate causal variants for ovarian cancer risk modification was identified (top SNP rs10124837, HR: 0.73, 95%CI: 0.68 to 0.79, p-value 2× 10-16). These variants were located up to 20 kb upstream of BNC2. In BRCA2 mutation carriers one region, up to 45 kb upstream of BNC2, and containing 100 correlated SNPs was identified as candidate causal (top SNP rs62543585, HR: 0.69, 95%CI: 0.59 to 0.80, p-value 1.0 × 10-6). The candidate causal in BRCA1 mutation carriers did not include the strongest associated variant at this locus in the general population. In sum, we identified a set of candidate causal variants in a region that encompasses the BNC2 transcription start site. The ovarian cancer association at 9p22.2 may be mediated by different variants in BRCA1 mutation carriers and in the general population. Thus, potentially different mechanisms may underlie ovarian cancer risk for mutation carriers and the general population.

The Family of Object-Related Depictives in English and Spanish: Towards a Usage-Based Constructionist Analysis

ERIC Educational Resources Information Center

Gonzalvez-Garcia, Francisco

2009-01-01

Drawing on naturally-occurring data extracted from the British National Corpus (BNC) and the Corpus de Referencia del Espanol Actual (CREA) in conjunction with data elicited from native speakers by means of questionnaires, this paper provides a bottom-up, usage-based analysis of instances of depictive secondary predicates involving mainly verba…

Is There a Core General Vocabulary? Introducing the "New General Service List"

ERIC Educational Resources Information Center

Brezina, Vaclav; Gablasova, Dana

2015-01-01

The current study presents a "New General Service List (new-GSL)", which is a result of robust comparison of four language corpora ("LOB," "BNC," "BE06," and "EnTenTen12") of the total size of over 12 billion running words. The four corpora were selected to represent a variety of corpus sizes and…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kung, C C; Brunkhorst, C; Greenough, N

Experimental results have shown that the high harmonic fast wave (HHFW) at 30 MHz can provide substantial plasma heating and current drive for the NSTX spherical tokamak operation. However, the present antenna strap design rarely achieves the design goal of delivering the full transmitter capability of 6 MW to the plasma. In order to deliver more power to the plasma, a new antenna strap design and the associated coaxial line feeds are being constructed. This new antenna strap design features two feedthroughs to replace the old single feed-through design. In the design process, CST Microwave Studio has been used tomore » simulate the entire new antenna strap structure including the enclosure and the Faraday shield. In this paper, the antenna strap model and the simulation results will be discussed in detail. The test results from the new antenna straps with their associated resonant loops will be presented as well.« less

Qualification and cryogenic performance of cryomodule components at CEBAF

DOE Office of Scientific and Technical Information (OSTI.GOV)

Heckman, J.; Macha, K.; Fischer, J.

1996-12-31

At CEBAF an electron beam is accelerated by superconducting resonant niobium cavities which are operated submerged in superfluid helium. The accelerator has 42 1/4 cryomodules, each containing eight cavities. The qualification and design of components for the cryomodules under went stringent testing and evaluation for acceptance. Indium wire seals are used between the cavity and helium vessel interface to make a superfluid helium leak tight seal. Each cavity is equipped with a mechanical tuner assembly designed to stretch and compress the cavities. Two rotary feedthroughs are used to operate each mechanical tuner assembly. Ceramic feedthroughs not designed for super-fluid weremore » qualified for tuner and cryogenic instrumentation. To ensure long term integrity of the machine special attention is required for material specifications and machine processes. The following is to share the qualification methods, design and performance of the cryogenic cryomodule components.« less

Development of a full-length external-fuel thermionic converter for in-pile testing.

NASA Technical Reports Server (NTRS)

Schock, A.; Raab, B.

1971-01-01

Description of an external-fuel thermionic converter which utilizes a thoriated-tungsten fuel-emitter body. Performance in out-of-pile tests was comparable to that of an arc-cast tungsten emitter body, with 400-eW output power (about 5 W/sq cm) at 10.8% efficiency. Maximum fuel clad temperature averaged from 1650 to 1700 C during the 300-hour test. This converter has been processed for in-pile testing. The various processing steps, including the installation of six emitter thermocouples, encapsulation in the secondary container, and joining to the fission-gas collection system, are described in detail. In addition to the converter assembly, a doubly contained fission gas collection assembly with radiation-hardened differential pressure transducers was fabricated. The experiment support plate required for the in-pile test, containing electrically insulated instrumentation feedthroughs and coolant line feedthroughs to the vacuum test chamber, was also fabricated.

Multiple internal seal right micro-electro-mechanical system vacuum package

NASA Technical Reports Server (NTRS)

Shcheglov, Kirill V. (Inventor); Wiberg, Dean V. (Inventor); Hayworth, Ken J. (Inventor); Yee, Karl Y. (Inventor); Bae, Youngsam (Inventor); Challoner, A. Dorian (Inventor); Peay, Chris S. (Inventor)

2007-01-01

A Multiple Internal Seal Ring (MISR) Micro-Electro-Mechanical System (MEMS) vacuum package that hermetically seals MEMS devices using MISR. The method bonds a capping plate having metal seal rings to a base plate having metal seal rings by wafer bonding the capping plate wafer to the base plate wafer. Bulk electrodes may be used to provide conductive paths between the seal rings on the base plate and the capping plate. All seals are made using only metal-to-metal seal rings deposited on the polished surfaces of the base plate and capping plate wafers. However, multiple electrical feed-through metal traces are provided by fabricating via holes through the capping plate for electrical connection from the outside of the package through the via-holes to the inside of the package. Each metal seal ring serves the dual purposes of hermetic sealing and providing the electrical feed-through metal trace.

Insulation Test Cryostat with Lift Mechanism

NASA Technical Reports Server (NTRS)

Dokos, Adam G. (Inventor); Fesmire, James E. (Inventor)

2014-01-01

A multi-purpose, cylindrical thermal insulation test apparatus is used for testing insulation materials and systems of materials using a liquid boil-off calorimeter system for absolute measurement of the effective thermal conductivity (k-value) and heat flux of a specimen material at a fixed environmental condition (cold-side temperature, warm-side temperature, vacuum pressure level, and residual gas composition). The apparatus includes an inner vessel for receiving a liquid with a normal boiling point below ambient temperature, such as liquid nitrogen, enclosed within a vacuum chamber. A cold mass assembly, including the upper and lower guard chambers and a middle test vessel, is suspended from a lid of the vacuum canister. Each of the three chambers is filled and vented through a single feedthrough. All fluid and instrumentation feedthroughs are mounted and suspended from a top domed lid to allow easy removal of the cold mass. A lift mechanism allows manipulation of the cold mass assembly and insulation test article.

Multiple internal seal ring micro-electro-mechanical system vacuum packaging method

NASA Technical Reports Server (NTRS)

Hayworth, Ken J. (Inventor); Yee, Karl Y. (Inventor); Shcheglov, Kirill V. (Inventor); Bae, Youngsam (Inventor); Wiberg, Dean V. (Inventor); Challoner, A. Dorian (Inventor); Peay, Chris S. (Inventor)

2008-01-01

A Multiple Internal Seal Ring (MISR) Micro-Electro-Mechanical System (MEMS) vacuum packaging method that hermetically seals MEMS devices using MISR. The method bonds a capping plate having metal seal rings to a base plate having metal seal rings by wafer bonding the capping plate wafer to the base plate wafer. Bulk electrodes may be used to provide conductive paths between the seal rings on the base plate and the capping plate. All seals are made using only metal-to-metal seal rings deposited on the polished surfaces of the base plate and capping plate wafers. However, multiple electrical feed-through metal traces are provided by fabricating via holes through the capping plate for electrical connection from the outside of the package through the via-holes to the inside of the package. Each metal seal ring serves the dual purposes of hermetic sealing and providing the electrical feed-through metal trace.

Insulation Test Cryostat with Lift Mechanism

NASA Technical Reports Server (NTRS)

Fesmire, James E. (Inventor); Dokos, Adam G. (Inventor)

2016-01-01

A multi-purpose, cylindrical thermal insulation test apparatus is used for testing insulation materials and systems of materials using a liquid boil-off calorimeter system for absolute measurement of the effective thermal conductivity (k-value) and heat flux of a specimen material at a fixed environmental condition (cold-side temperature, warm-side temperature, vacuum pressure level, and residual gas composition). An inner vessel receives liquid with a normal boiling point below ambient temperature, such as liquid nitrogen, enclosed within a vacuum chamber. A cold mass assembly, including upper and lower guard chambers and middle test vessel, is suspended from a lid of the vacuum canister. Each of the three chambers is filled and vented through a single feedthrough. All fluid and instrumentation feedthroughs are mounted and suspended from a top domed lid allowing easy removal of the cold mass. A lift mechanism allows manipulation of the cold mass assembly and insulation test article.

Performance degradation of ferrofluidic feedthroughs in a mixed irradiation field

DOE PAGES

Simos, Nikolaos; Fernandes, S.; Mittig, Wolfgang; ...

2016-10-06

We present ferrofluidic feedthrough (FF) rotary seals containing either NdFeB or SmCo-type permanent magnets that have been considered for use in the target and beam dump systems of the Facility for Rare Isotope Beams (FRIB). To evaluate their performance under irradiation three FF seals were irradiated in a mixed field consisting of fast neutrons, protons and γ-rays to an average absorbed dose of 0.2, 2.0, and 20.0 MGy at the Brookhaven Linac Isotope Producer facility (BLIP). The radiation types and energy profiles mimic those expected at the FRIB facility. Degradation of the operational performance of these devices due to irradiationmore » is expected to be the result of the de-magnetization of the permanent magnets contained within the seal and the changes in the ferrofluid properties. Post-irradiation performance was evaluated by determining the ferrofluidic seal vacuum tightness and torque under static and dynamic conditions. The study revealed that the ferrofluidic feedthrough seal irradiated to a dose of 0.2 MGy maintained its vacuum tightness under both static and rotational condition while the one irradiated to a dose of 2.0 MGy exhibited signs of ferrofluid damage but no overall performance loss. Lastly, at 20 MGy dose the effects of irradiation on the ferrofluid properties (viscosity and particle agglomeration) were shown to be severe. Furthermore, limited de-magnetization of the annular shaped Nd 2Fe 14B and Sm 2Co 17 magnets located within the irradiated FFs was observed for doses of 0.2 MGy and 20 MGy respectively.« less

Stretched Verb Collocations with "Give": Their Use and Translation into Spanish Using the BNC and CREA Corpora

ERIC Educational Resources Information Center

Molina-Plaza, Silvia; de Gregorio-Godeo, Eduardo

2010-01-01

Within the context of on-going research, this paper explores the pedagogical implications of contrastive analyses of multiword units in English and Spanish based on electronic corpora as a CALL resource. The main tenets of collocations from a contrastive perspective--and the points of contact and departure between both languages--are discussed…

In a Manner of Speaking: Assessing Frequent Spoken Figurative Idioms to Assist ESL/EFL Teachers

ERIC Educational Resources Information Center

Grant, Lynn E.

2007-01-01

This article outlines criteria to define a figurative idiom, and then compares the frequent figurative idioms identified in two sources of spoken American English (academic and contemporary) to their frequency in spoken British English. This is done by searching the spoken part of the British National Corpus (BNC), to see whether they are frequent…

Analyzing Idioms and Their Frequency in Three Advanced ILI Textbooks: A Corpus-Based Study

ERIC Educational Resources Information Center

Alavi, Sepideh; Rajabpoor, Aboozar

2015-01-01

The present study aimed at identifying and quantifying the idioms used in three ILI "Advanced" level textbooks based on three different English corpora; MICASE, BNC and the Brown Corpus, and comparing the frequencies of the idioms across the three corpora. The first step of the study involved searching the books to find multi-word…

"Sort of" in British Women's and Men's Speech

ERIC Educational Resources Information Center

Miettinen, Hanna; Watson, Greg

2013-01-01

This paper (Note 1) examines the form sort of in British men and women's speech, and investigates whether there is a gender difference in the use of this form. We do so through corpus analysis of the British National Corpus (BNC). We contend there is no quantitative difference in the use of sort of in men and women's speech. Contrary to general…

Isolation of Neospora caninum from kidney and brain of a bovine foetus and molecular characterization in Brazil.

PubMed

Locatelli Dittrich, Rosangela; Regidor-Cerrillo, Javier; Ortega-Mora, Luis Miguel; Oliveira Koch, Marília de; Busch, Ana Paula B; Gonçalves, Kamila Alcalá; Cruz, Amilcar A

2018-02-01

Bovine neosporosis has become a disease of international concern as it is among the main causes of abortion in cattle. Viable N. caninum has been isolated from brains of fetuses and neonatal calves, and there is no report of isolation of tachyzoites from kidney. Also, detailed information about the genetic diversity of N. caninum is scarce. N. caninum tachyzoites were isolated from the kidney and the brain of an aborted 4-month-old bovine foetus. The parasite was confirmed to be N. caninum by PCR. The tachyzoites of the new isolate, named BNC-PR4, were propagated in Vero cell cultures. Pathogenicity of the parasite was examined in BALB/c mice. Mice inoculated intraperitoneally with BNC-PR4 failed to yield clinical signs of disease and did not induce severe brain lesions, suggesting a bovine isolate with low virulence. The N. caninum-positive DNA sample was further analyzed by multilocus microsatellite (MS) genotyping for MS4, MS5, MS6A, MS6B, MS7, MS8, MS10, MS12, and MS21. Multilocus-microsatellite genotyping revealed a unique genetic profile that differed from previously reported isolates. Published by Elsevier Inc.

Microdosimetric intercomparison of BNCT beams at BNL and MIT.

PubMed

Burmeister, Jay; Riley, Kent; Coderre, Jeffrey A; Harling, Otto K; Ma, Ruimei; Wielopolski, Lucian; Kota, Chandrasekhar; Maughan, Richard L

2003-08-01

Microdosimetric measurements have been performed at the clinical beam intensities in two epithermal neutron beams, the Brookhaven Medical Research Reactor and the M67 beam at the Massachusetts Institute of Technology Research Reactor, which have been used to treat patients with Boron Neutron Capture Therapy (BNCT). These measurements offer an independent assessment of the dosimetry used at these two facilities, as well as provide information about the radiation quality not obtainable from conventional macrodosimetric techniques. Moreover, they provide a direct measurement of the absorbed dose resulting from the BNC reaction. BNC absorbed doses measured within this study are approximately 15% lower than those estimated using foil activation at both MIT and BNL. Finally, an intercomparison of the characteristics and radiation quality of these two clinical beams is presented. The techniques described here allow an accurate quantitative comparison of the physical absorbed dose as well as a measure of the biological effectiveness of the absorbed dose delivered by different epithermal beams. No statistically significant differences were observed in the predicted RBEs of these two beams. The methodology presented here can help to facilitate the effective sharing of clinical results in an effort to demonstrate the clinical utility of BNCT.

Evaluation of oxidative stress and genotoxicity in battery manufacturing workers occupationally exposed to lead.

PubMed

Singh, Zorawar; Chadha, Pooja; Sharma, Suman

2013-01-01

Battery manufacturing workers are occupationally exposed to lead (Pb), which is a highly toxic heavy metal. The aim of this study was to investigate the blood lead levels (BLL) of 30 battery manufacturing workers and find the correlation between BLL, micronucleated cell (MNC) frequency, binucleated cell (BNC) frequency in buccal mucosal cells and malondialdehyde concentrations in serum. 30 subjects of the BMW group, exposed to lead, and 30 control subjects, matched with the exposed subjects with respect to age, socio-economic status, sex, diet, smoking and drinking habits, were monitored for this study. BLL was found to have highly significant difference between both the groups (P < 0.001). The serum MDA levels were observed at significantly higher levels (6.76 ± 3.26) for the exposed group as compared to the control group (2.10 ± 1.02; P < 0.001). Buccal micronucleus test showed that both MNC and BNC frequencies were higher among the workers, in comparison to the control subjects. A positive correlation has been found between BLL and all the parameters. Our results indicate an increased health associated risk for workers occupationally exposed to lead.

1KW Power Transmission Using Wireless Acoustic-Electric Feed-Through (WAEF)

NASA Technical Reports Server (NTRS)

Sherrit, S.; Bao, X.; Badescu, M.; Aldrich, J.; Bar-Cohen, Y.; Biederman, W.

2008-01-01

A variety of space applications require the delivery of power into sealed structures. Since the structural integrity can be degraded by holes for cabling we present an alternative method of delivering power and information using stress waves to the internal space of a sealed structure. One particular application of this technology is in sample return missions where it is critical to preserve the sample integrity and to prevent earth contamination. Therefore, the container has to be hermetically sealed and the integrity of the seal must be monitored in order to insure to a high degree of reliability the integrity of the sample return vessel. In this study we investigated the use of piezoelectric acoustic-electric power feed-through devices to transfer electric power wirelessly through a solid wall by using elastic or acoustic waves. The technology is applicable to a range of space and terrestrial applications where power is required by electronic equipment inside sealed containers, vacuum or pressure vessels, etc., where holes in the wall are prohibitive or may result in significant structural performance degradation or unnecessarily complex designs. To meet requirements of higher power applications, the feasibility to transfer kilowatts level power was investigated. Pre-stressed longitudinal piezoelectric feed-through devices were analyzed by finite element models and an equivalent circuit model was developed to predict the power transfer characteristics to different electric loads. Based on the results of the analysis a prototype device was designed, fabricated and a demonstration of the transmission of electric power up to 1.068-kW was successfully conducted. Efficiencies in the 80-90% range were also demonstrated and methods to increase the efficiency further are currently being considered.

Gadolinium-Based GaN for Neutron Detection with Gamma Discrimination

DTIC Science & Technology

2016-06-01

spectroscopy system: 1, Earth ground; 2, Shielding and vacuum chamber; 3, Probe station; 4, Ohmic contact; 5, Schottky Contact; 6, BNC cable; 7...NUMBER 6. AUTHOR( S ) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME( S ) AND ADDRESS(ES) 8...PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING / MONITORING AGENCY NAME( S ) AND ADDRESS(ES) 10. SPONSOR

Loanwords and Vocabulary Size Test Scores: A Case of Different Estimates for Different L1 Learners

ERIC Educational Resources Information Center

Laufer, Batia; McLean, Stuart

2016-01-01

The article investigated how the inclusion of loanwords in vocabulary size tests affected the test scores of two L1 groups of EFL learners: Hebrew and Japanese. New BNC- and COCA-based vocabulary size tests were constructed in three modalities: word form recall, word form recognition, and word meaning recall. Depending on the test modality, the…

Programmable bio-nano-chip system for saliva diagnostics

NASA Astrophysics Data System (ADS)

Christodoulides, Nicolaos; De La Garza, Richard; Simmons, Glennon W.; McRae, Michael P.; Wong, Jorge; Kosten, Thomas R.; Miller, Craig S.; Ebersole, Jeffrey L.; McDevitt, John

2014-06-01

This manuscript describes programmable Bio-Nano-Chip (p-BNC) approach that serves as miniaturized assay platform designed for the rapid detection and quantitation of multiple analytes in biological fluids along with the specific applications in salivary diagnostics intended for the point of need (PON). Included here are oral fluid-based tests for local periodontal disease, systemic cardiac disease and multiplexed tests for drugs of abuse.

Environmental Security: What Environmental Issues Impact Regional Stability and Affect United States Foreign Policy with Mexico?

DTIC Science & Technology

1997-03-01

extraregional sponsor of Alianza para el Desarrollo Sostenible (Sustainable Development Alliance - ALIDES), along with United States and Canada. ALIDES is...more stable the government the better the country will be able to solve its environmental problems. 42 Glossary ALIDES Alianza para el Desarrollo ... Sostenible (Sustainable Development Alliance) C Centigrade CFCs Chlorofluorocarbons BECC Border Environment Cooperation Commission BNC Binational

QTL mapping for quinone reductase activity in broccoli with Hepa1c1c7 cell lines

USDA-ARS?s Scientific Manuscript database

Floret tissue from 125 F2:3 broccoli families derived from the cross 'VI-158 x Brocolette Neri E. Cespuglio (BNC)' was harvested in 2009. Tissue was freeze-dried and stored in the dark at -80 until use. Distilled water was added to floret tissue (50 mg/mL) and auto-hydrolyzed for 24 hours in room te...

Hapten optimization for cocaine vaccine with improved cocaine recognition.

PubMed

Ramakrishnan, Muthu; Kinsey, Berma M; Singh, Rana A; Kosten, Thomas R; Orson, Frank M

2014-09-01

In the absence of any effective pharmacotherapy for cocaine addiction, immunotherapy is being actively pursued as a therapeutic intervention. While several different cocaine haptens have been explored to develop anticocaine antibodies, none of the hapten was successfully designed, which had a protonated tropane nitrogen as is found in native cocaine under physiological conditions, including the succinyl norcocaine (SNC) hapten that has been tested in phase II clinical trials. Herein, we discuss three different cocaine haptens: hexyl norcocaine (HNC), bromoacetamido butyl norcocaine (BNC), and succinyl butyl norcocaine (SBNC), each with a tertiary nitrogen structure mimicking that of native cocaine which could optimize the specificity of anticocaine antibodies for better cocaine recognition. Mice immunized with these haptens conjugated to immunogenic proteins produced high titre anticocaine antibodies. However, during chemical conjugation of HNC and BNC haptens to carrier proteins, the 2β methyl ester group is hydrolyzed, and immunizing mice with these conjugate vaccines in mice produced antibodies that bound both cocaine and the inactive benzoylecgonine metabolite. While in the case of the SBNC conjugate, vaccine hydrolysis of the methyl ester did not appear to occur, leading to antibodies with high specificity to cocaine over BE. Although we observed similar specificity with a SNC hapten, the striking difference is that SBNC carries a positive charge on the tropane nitrogen atom, and therefore, it is expected to have better binding of cocaine. The 50% cocaine inhibitory concentration (IC50 ) value for SBNC antibodies (2.8 μm) was significantly better than the SNC antibodies (9.4 μm) when respective hapten-BSA was used as a substrate. In addition, antibodies from both sera had no inhibitory effect from BE. In contrast to BNC and HNC, the SBNC conjugate was also found to be highly stable without any noticeable hydrolysis for several months at 4 °C and 2-3 days in pH 10 buffer at 37 °C. © Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

A Genome-Wide Association Study Identifies a Novel Major Locus for Glycemic Control in Type 1 Diabetes, as Measured by Both A1C and Glucose

PubMed Central

Paterson, Andrew D.; Waggott, Daryl; Boright, Andrew P.; Hosseini, S. Mohsen; Shen, Enqing; Sylvestre, Marie-Pierre; Wong, Isidro; Bharaj, Bhupinder; Cleary, Patricia A.; Lachin, John M.; Below, Jennifer E.; Nicolae, Dan; Cox, Nancy J.; Canty, Angelo J.; Sun, Lei; Bull, Shelley B.

2010-01-01

OBJECTIVE Glycemia is a major risk factor for the development of long-term complications in type 1 diabetes; however, no specific genetic loci have been identified for glycemic control in individuals with type 1 diabetes. To identify such loci in type 1 diabetes, we analyzed longitudinal repeated measures of A1C from the Diabetes Control and Complications Trial. RESEARCH DESIGN AND METHODS We performed a genome-wide association study using the mean of quarterly A1C values measured over 6.5 years, separately in the conventional (n = 667) and intensive (n = 637) treatment groups of the DCCT. At loci of interest, linear mixed models were used to take advantage of all the repeated measures. We then assessed the association of these loci with capillary glucose and repeated measures of multiple complications of diabetes. RESULTS We identified a major locus for A1C levels in the conventional treatment group near SORCS1 (10q25.1, P = 7 × 10−10), which was also associated with mean glucose (P = 2 × 10−5). This was confirmed using A1C in the intensive treatment group (P = 0.01). Other loci achieved evidence close to genome-wide significance: 14q32.13 (GSC) and 9p22 (BNC2) in the combined treatment groups and 15q21.3 (WDR72) in the intensive group. Further, these loci gave evidence for association with diabetic complications, specifically SORCS1 with hypoglycemia and BNC2 with renal and retinal complications. We replicated the SORCS1 association in Genetics of Diabetes in Kidneys (GoKinD) study control subjects (P = 0.01) and the BNC2 association with A1C in nondiabetic individuals. CONCLUSIONS A major locus for A1C and glucose in individuals with diabetes is near SORCS1. This may influence the design and analysis of genetic studies attempting to identify risk factors for long-term diabetic complications. PMID:19875614

Indium sealing techniques.

NASA Technical Reports Server (NTRS)

Hochuli, U.; Haldemann, P.

1972-01-01

Gold films are used as an alloying flux to form 5-micron-thick indium film seals at temperatures below 300 C. Pyrex was sealed to quartz, ULE, CER-VIT, Irtran 2, Ge, GaAs, Invar, Kovar, Al, and Cu. The seals can also be used as current feedthroughs and graded seals.

Advanced Dynamic Anthropomorphic Manikin (ADAM) Final Design Report

DTIC Science & Technology

1990-03-01

an ejection sequence, the human body is subjected to numerous dynamic loadings from the catapult and sustaining rocket, as well as from wind blast. In...ML12S33 CMF’I.B #1.33, (A6) Selection = 3? BNC.S MU2S34 No - check for 4 LEA.L F’ARMSG,A2 Else display Parity prompt MOVC..W PARCT,D2 BSFR DI.; PMSG CLR.W

Telescope Fabra ROA Montsec: A New Robotic Wide Field Baker-Nunn Facility

NASA Astrophysics Data System (ADS)

Fors, Octavi; Núñez, Jorge; Muiños, José Luis; Montojo, Francisco Javier; Baena-Gallé, Roberto; Boloix, Jaime; Morcillo, Ricardo; Merino, María Teresa; Downey, Elwood C.; Mazur, Michael J.

2013-05-01

A Baker-Nunn Camera (BNC), originally installed at the Real Instituto y Observatorio de la Armada (ROA) in 1958, was refurbished and robotized. The new facility, called Telescope Fabra ROA Montsec (TFRM), was installed at the Observatori Astronòmic del Montsec (OAdM). The process of refurbishment is described in detail. Most of the steps of the refurbishment project were accomplished by purchasing commercial components, which involve little posterior engineering assembling work. The TFRM is a 0.5 m aperture f/0.96 optically modified BNC, which offers a unique combination of instrumental specifications: fully robotic and remote operation, wide field of view (4°.4 × 4°.4), moderate limiting magnitude (V ˜ 19.5 mag), ability of tracking at arbitrary right ascension (α) and declination (δ) rates, as well as opening and closing CCD shutter at will during an exposure. Nearly all kinds of image survey programs can benefit from those specifications. Apart from other less time-consuming programs, since the beginning of science TFRM operations we have been conducting two specific and distinct surveys: super-Earths transiting around M-type dwarfs stars, and geostationary debris in the context of Space Situational Awareness/Space Surveillance and Tracking (SSA/SST) programs. Preliminary results for both cases will be shown.

Major cluster mergers and the location of the brightest cluster galaxy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martel, Hugo; Robichaud, Fidèle; Barai, Paramita, E-mail: Hugo.Martel@phy.ulaval.ca

Using a large N-body cosmological simulation combined with a subgrid treatment of galaxy formation, merging, and tidal destruction, we study the formation and evolution of the galaxy and cluster population in a comoving volume (100 Mpc){sup 3} in a ΛCDM universe. At z = 0, our computational volume contains 1788 clusters with mass M {sub cl} > 1.1 × 10{sup 12} M {sub ☉}, including 18 massive clusters with M {sub cl} > 10{sup 14} M {sub ☉}. It also contains 1, 088, 797 galaxies with mass M {sub gal} ≥ 2 × 10{sup 9} M {sub ☉} and luminositymore » L > 9.5 × 10{sup 5} L {sub ☉}. For each cluster, we identified the brightest cluster galaxy (BCG). We then computed two separate statistics: the fraction f {sub BNC} of clusters in which the BCG is not the closest galaxy to the center of the cluster in projection, and the ratio Δv/σ, where Δv is the difference in radial velocity between the BCG and the whole cluster and σ is the radial velocity dispersion of the cluster. We found that f {sub BNC} increases from 0.05 for low-mass clusters (M {sub cl} ∼ 10{sup 12} M {sub ☉}) to 0.5 for high-mass clusters (M {sub cl} > 10{sup 14} M {sub ☉}) with very little dependence on cluster redshift. Most of this result turns out to be a projection effect and when we consider three-dimensional distances instead of projected distances, f {sub BNC} increases only to 0.2 at high-cluster mass. The values of Δv/σ vary from 0 to 1.8, with median values in the range 0.03-0.15 when considering all clusters, and 0.12-0.31 when considering only massive clusters. These results are consistent with previous observational studies and indicate that the central galaxy paradigm, which states that the BCG should be at rest at the center of the cluster, is usually valid, but exceptions are too common to be ignored. We built merger trees for the 18 most massive clusters in the simulation. Analysis of these trees reveal that 16 of these clusters have experienced 1 or several major or semi-major mergers in the past. These mergers leave each cluster in a non-equilibrium state, but eventually the cluster settles into an equilibrium configuration, unless it is disturbed by another major or semi-major merger. We found evidence that these mergers are responsible for the off-center positions and peculiar velocities of some BCGs. Our results thus support the merging-group scenario, in which some clusters form by the merging of smaller groups in which the galaxies have already formed, including the galaxy destined to become the BCG. Finally, we argue that f {sub BNC} is not a very robust statistics, as it is very sensitive to projection and selection effects, but that Δv/σ is more robust. Still, both statistics exhibit a signature of major mergers between clusters of galaxies.« less

A Method for Correcting Broken Hyphenations in Noisy English Text

DTIC Science & Technology

2012-04-01

words, such as a frequency list . An algorithm that would make use of word validation, taking into account the various usages of hyphens in English, is...commas, and question marks from the surrounding words. The British National Corpus (2) (BNC) frequency list was used to perform the validation...rather than a separate spell checking program. This was primarily because implementation of the algorithm using a frequency list was quite trivial

Effect of component compression on the initial performance of an IPV nickel-hydrogen cell

NASA Technical Reports Server (NTRS)

Gahn, Randall F.

1987-01-01

An experimental method was developed for evaluating the effect of component compression on the charge and discharge voltage characteristics of a 3 1/2 in. diameter boiler plate cell. A standard boiler plate pressure vessel was modified by the addition of a mechanical feedthrough on the bottom of the vessel which permitted different compressions to be applied to the components without disturbing the integrity of the stack. Compression loadings from 0.94 to 27.4 psi were applied by suspending weights from the feedthrough rod. Cell voltages were measured for 0.96-C, 55-min charge and for 1.37-C, 35-min and 2-C, 24-min discharges. An initial change in voltage performance on both charge and discharge as the loading increased was attributed to seating of the components. Subsequent variation of the compression from 2.97 to 27.4 psi caused only minor changes in either the charge or the discharge voltages. Several one month open-circuit voltage stands and 1100 cycles under LEO conditions at the maximum loading have produced no change in performance.

Effect of component compression on the initial performance of an IPV nickel-hydrogen cell. [Individual Pressure Vessel

NASA Technical Reports Server (NTRS)

Gahn, Randall F.

1987-01-01

An experimental method was developed for evaluating the effect of component compression on the charge and discharge voltage characteristics of a 3 1/2 in. diameter boiler plate cell. A standard boiler plate pressure vessel was modified by the addition of a mechanical feedthrough on the bottom of the vessel which permitted different compressions to be applied to the components without disturbing the integrity of the stack. Compression loadings from 0.94 to 27.4 psi were applied by suspending weights from the feedthrough rod. Cell voltages were measured for 0.96-C, 55-min charge and for 1.37-C, 35-min and 2-C, 24-min discharges. An initial change in voltage performance on both charge and discharge as the loading increased was attributed to seating of the components. Subsequent variation of the compression from 2.97 to 27.4 psi caused only minor changes in either the charge or the discharge voltages. Several one month open-circuit voltage stands and 1100 cycles under LEO conditions at the maximum loading have produced no change in performance.

An integrated wire harp and readout electronics inside vacuum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chatterjee, Mou; Nabhiraj, P. Y.

A wire harp is a well known instrument used in ion beam profile measurement and beam diagnostics. Till date, for beam instrumentation, the harp is placed inside the vacuum chamber or beam line in direct exposure to the beam profile to be measured, whereas the related readout electronics is placed outside somewhere at a convenient place. Here, a harp has been developed along with the readout electronics as an integrated part of it and both were placed inside the beam line vacuum (order of 10{sup −7} Torr) to make the system much simpler, easy to operate, and measure small beammore » current more accurately. The entire signal conversion and processing is done inside the vacuum unlike other systems; hence, the electronics is kept inside. This results in a lesser number (only 4 pin) of electrical connections (feedthrough) including power which otherwise would have required 32 feedthrough pins only for signal readout for a 13 × 13 (X × Y) channel harp. This paper describes a completely new approach to the design of a conventional beam harp widely used for beam instrumentation.« less

Modeling of Low Feed-Through CD Mix Implosions

NASA Astrophysics Data System (ADS)

Pino, Jesse; MacLaren, Steven; Greenough, Jeff; Casey, Daniel; Dittrich, Tom; Kahn, Shahab; Kyrala, George; Ma, Tammy; Salmonson, Jay; Smalyuk, Vladimir; Tipton, Robert

2015-11-01

The CD Mix campaign previously demonstrated the use of nuclear diagnostics to study the mix of separated reactants in plastic capsule implosions at the National Ignition Facility. However, the previous implosions suffered from large instability growth seeded from perturbations on the outside of the capsule. Recently, the separated reactants technique has been applied to two platforms designed to minimize this feed-through and isolate local mix at the gas-ablator interface: the Two Shock (TS) and Adiabat-Shaped (AS) Platforms. Additionally, the background contamination of Deuterium in the gas has been greatly reduced, allowing for simultaneous observation of TT, DT, and DD neutrons, which respectively give information about core gas performance, gas-shell atomic mix, and heating of the shell. In this talk, we describe efforts to model these implosions using high-resolution 2D ARES simulations with both a Reynolds-Averaged Navier Stokes method and an enhanced diffusivity model. This work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344. LLNL-ABS-674867.

An integrated wire harp and readout electronics inside vacuum.

PubMed

Chatterjee, Mou; Nabhiraj, P Y

2015-03-01

A wire harp is a well known instrument used in ion beam profile measurement and beam diagnostics. Till date, for beam instrumentation, the harp is placed inside the vacuum chamber or beam line in direct exposure to the beam profile to be measured, whereas the related readout electronics is placed outside somewhere at a convenient place. Here, a harp has been developed along with the readout electronics as an integrated part of it and both were placed inside the beam line vacuum (order of 10(-7) Torr) to make the system much simpler, easy to operate, and measure small beam current more accurately. The entire signal conversion and processing is done inside the vacuum unlike other systems; hence, the electronics is kept inside. This results in a lesser number (only 4 pin) of electrical connections (feedthrough) including power which otherwise would have required 32 feedthrough pins only for signal readout for a 13 × 13 (X × Y) channel harp. This paper describes a completely new approach to the design of a conventional beam harp widely used for beam instrumentation.

An integrated wire harp and readout electronics inside vacuum

NASA Astrophysics Data System (ADS)

Chatterjee, Mou; Nabhiraj, P. Y.

2015-03-01

A wire harp is a well known instrument used in ion beam profile measurement and beam diagnostics. Till date, for beam instrumentation, the harp is placed inside the vacuum chamber or beam line in direct exposure to the beam profile to be measured, whereas the related readout electronics is placed outside somewhere at a convenient place. Here, a harp has been developed along with the readout electronics as an integrated part of it and both were placed inside the beam line vacuum (order of 10-7 Torr) to make the system much simpler, easy to operate, and measure small beam current more accurately. The entire signal conversion and processing is done inside the vacuum unlike other systems; hence, the electronics is kept inside. This results in a lesser number (only 4 pin) of electrical connections (feedthrough) including power which otherwise would have required 32 feedthrough pins only for signal readout for a 13 × 13 (X × Y) channel harp. This paper describes a completely new approach to the design of a conventional beam harp widely used for beam instrumentation.

Upgrades to the NSTX HHFW antenna

NASA Astrophysics Data System (ADS)

Ellis, R.; Brunkhorst, C.; Hosea, J.

2014-02-01

The High Harmonic Fast Wave (HHFW) antenna for the National Spherical Torus Experiment (NSTX) at PPPL will be upgraded as part of the NSTX upgrade project. Higher magnetic fields and plasma current result in disruption forces on the current straps that can be up to four times the original design values. The current straps on the HHFW antenna are presently fed by coaxial feedthroughs with rigid center conductors. The additional forces on the current straps require a compliant section in the center conductor in order to minimize the forces on the feedthrough. The design of this compliant section has been an integrated effort involving electrostatic calculations in parallel with mechanical and thermal analyses, in order to arrive at a design that is optimized for mechanical, thermal and electrical considerations. The voltage standoff obtained from this design will be verified when a prototype antenna is evaluated on our RF test stand. This paper describes the design of the compliant section of the center conductor, mechanical, thermal and electrostatic calculations, and plans for full implementation of the upgrade on NSTX.

Acoustic Mechanical Feedthroughs

NASA Technical Reports Server (NTRS)

Sherrit, Stewart; Walkemeyer, Phillip; Bao, Xiaoqi; Bar-Cohen, Yoseph; Badescu, Mircea

2013-01-01

Electromagnetic motors can have problems when operating in extreme environments. In addition, if one needs to do mechanical work outside a structure, electrical feedthroughs are required to transport the electric power to drive the motor. In this paper, we present designs for driving rotary and linear motors by pumping stress waves across a structure or barrier. We accomplish this by designing a piezoelectric actuator on one side of the structure and a resonance structure that is matched to the piezoelectric resonance of the actuator on the other side. Typically, piezoelectric motors can be designed with high torques and lower speeds without the need for gears. One can also use other actuation materials such as electrostrictive, or magnetostrictive materials in a benign environment and transmit the power in acoustic form as a stress wave and actuate mechanisms that are external to the benign environment. This technology removes the need to perforate a structure and allows work to be done directly on the other side of a structure without the use of electrical feedthroughs, which can weaken the structure, pipe, or vessel. Acoustic energy is pumped as a stress wave at a set frequency or range of frequencies to produce rotary or linear motion in a structure. This method of transferring useful mechanical work across solid barriers by pumping acoustic energy through a resonant structure features the ability to transfer work (rotary or linear motion) across pressure or thermal barriers, or in a sterile environment, without generating contaminants. Reflectors in the wall of barriers can be designed to enhance the efficiency of the energy/power transmission. The method features the ability to produce a bi-directional driving mechanism using higher-mode resonances. There are a variety of applications where the presence of a motor is complicated by thermal or chemical environments that would be hostile to the motor components and reduce life and, in some instances, not be feasible. A variety of designs that have been designed, fabricated and tested will be presented

Studies of acoustic-electric feed-throughs for power transmission through structures

NASA Technical Reports Server (NTRS)

Sherrit, Stewart; Doty, Benjamin; Badescu, Mircea; Bao, Xiaoqi; Bar-Cohen, Yoseph; Aldrich, Jack; Chang, Zensheu

2006-01-01

There are numerous engineering design problems where the use of wires to transfer power and communicate data thru the walls of a structure is prohibitive or significantly difficult that it may require a complex design. Using physical feedthroughs in such systems may make them susceptible to leakage of chemicals or gasses, loss of pressure or vacuum, as well as difficulties in providing adequate thermal or electrical insulation. Moreover, feeding wires thru a wall of a structure reduces the strength of the structure and makes the structure prone to cracking due to fatigue that can result from cyclic loading and stress concentrations. One area that has already been identified to require a wireless alternative to electrical feedthroughs is the container of the Mars Sample Return Mission, which will need wireless sensors to sense a pressure leak and to avoid potential contamination. The idea of using elastic or acoustic waves to transfer power was suggested recently by [Y. Hu, et al., July 2003]. This system allows for the avoidance of cabling or wiring. The technology is applicable to the transfer of power for actuation, sensing and other tasks inside any sealed container or vacuum/pressure vessel. An alternative approach to the modeling presented previously [Sherrit et a., 2005] used network analysis to solve the same problem in a clear and expandable manner. Experimental tests on three different designs of these devices were performed. The three designs used different methods of coupling the piezoelectric element to the wall. In the first test the piezoelectric material was bolted using a backing structure. In the second test the piezoelectric was clamped after the application of grease and finally the piezoelectric element was attached using a conductive epoxy. The mechanical clamp with grease produced the highest measured efficiency of 53% however this design was the least practical from a fabrication viewpoint. The power transfer efficiency of conductive epoxy joint was 40% and the stress bolts (12%).

Anomalous scaling of Δ C versus T c in the Fe-based superconductors: the $${S}_{\\pm }$$-wave pairing state model

DOE PAGES

Bang, Yunkyu; Stewart, G. R.

2016-02-01

The strong power law behavior of the specific heat jumpmore » $${\\rm{\\Delta }}C\\;$$ versus T c $$({\\rm{\\Delta }}C/{T}_{{\\rm{c}}}\\sim {T}_{{\\rm{c}}}^{\\alpha },\\alpha \\approx 2)$$, first observed by Bud'ko et al (2009 Phys. Rev. B 79 220516), has been confirmed with several families of the Fe-based superconducting compounds with various dopings. We tested a minimal two band BCS model to understand this anomalous behavior and showed that this non-BCS relation between $${\\rm{\\Delta }}C\\;$$ versus T c is a generic property of the multiband superconducting state paired by a dominant interband interaction ($${V}_{\\mathrm{inter}}\\gt {V}_{\\mathrm{intra}}$$) reflecting the relation $$\\frac{{{\\rm{\\Delta }}}_{{\\rm{h}}}}{{{\\rm{\\Delta }}}_{{\\rm{e}}}}\\sim \\sqrt{\\frac{{N}_{{\\rm{e}}}}{{N}_{{\\rm{h}}}}}$$ near T c, as in the $${S}_{\\pm }$$-wave pairing state. We also found that this $${\\rm{\\Delta }}C\\;$$ versus T c power law can continuously change from the ideal BNC scaling to a considerable deviation by a moderate variation of the impurity scattering rate $${{\\rm{\\Gamma }}}_{0}$$ (non-pair-breaking). Finally, as a result, our model provides a consistent explanation why the electron-doped Fe-based superconductors follow the ideal BNC scaling very well while the hole-doped systems often show varying degree of deviations.« less

Using the MicroASAR on the NASA SIERRA UAS in the Characterization of Arctic Sea Ice Experiment

DTIC Science & Technology

2010-05-01

such that the dechirped signal is at an intermediate frequency. Feedthrough rejection is done by a surface acoustic wave (SAW) filter with its first...Fig. 3. NASA SIERRA UAS 3-View and Specifications • Up-looking and down-looking shortwave spectrometers. • Down-looking temperature sensors ( pyrometers

Ultrasonic Determination of Combinations of Third-Order Elastic Constants of Small Cubic Single Crystals

DTIC Science & Technology

1981-05-01

crystals Cesium cadmium fluoride Ultrasonic wave propagation Potassium zinc fluoride Nonlinear acoustics 20. A’?S1 RACT (Continue on reverse side If...is the stray capacitance of the detector, L is the inductance of the wire leading from the banana jack to the BNC connector (shown in Figure 111-2). Z...The samples on which measurements were made included [lO0] and [1111 copper samples, a sample of potassium zinc fluoride (KZnF 3 ) and a sample of

Mix Model Comparison of Low Feed-Through Implosions

NASA Astrophysics Data System (ADS)

Pino, Jesse; MacLaren, S.; Greenough, J.; Casey, D.; Dewald, E.; Dittrich, T.; Khan, S.; Ma, T.; Sacks, R.; Salmonson, J.; Smalyuk, V.; Tipton, R.; Kyrala, G.

2016-10-01

The CD Mix campaign previously demonstrated the use of nuclear diagnostics to study the mix of separated reactants in plastic capsule implosions at the NIF. Recently, the separated reactants technique has been applied to the Two Shock (TS) implosion platform, which is designed to minimize this feed-through and isolate local mix at the gas-ablator interface and produce core yields in good agreement with 1D clean simulations. The effects of both inner surface roughness and convergence ratio have been probed. The TT, DT, and DD neutron signals respectively give information about core gas performance, gas-shell atomic mix, and heating of the shell. In this talk, we describe efforts to model these implosions using high-resolution 2D ARES simulations. Various methods of interfacial mix will be considered, including the Reynolds-Averaged Navier Stokes (RANS) KL method as well as and a multicomponent enhanced diffusivity model with species, thermal, and pressure gradient terms. We also give predictions of a upcoming campaign to investigate Mid-Z mixing by adding a Ge dopant to the CD layer. LLNL-ABS-697251 This work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

Lysine 362 in cytochrome c oxidase regulates opening of the K-channel via changes in pKA and conformation.

PubMed

Woelke, Anna Lena; Galstyan, Gegham; Knapp, Ernst-Walter

2014-12-01

The metabolism of aerobic life uses the conversion of molecular oxygen to water as an energy source. This reaction is catalyzed by cytochrome e oxidase (CeO) consuming four electrons and four protons, which move along specific routes. While all four electrons are transferred via the same cofactors to the binuclear reaction center (BNC), the protons take two different routes in the A-type CeO, i.e., two of the four chemical protons consumed in the reaction arrive via the D-channel in the oxidative first half starting after oxygen binding. The other two chemical protons enter via the K-channel in the reductive second half of the reaction cycle. To date, the mechanism behind these separate proton transport pathways has not been understood. In this study, we propose a model that can explain the reaction-step specific opening and closing of the K-channel by conformational and pKA changes of its central lysine 362. Molecular dynamics simulations reveal an upward movement of Lys362 towards the BNC, which had already been supposed by several experimental studies. Redox state-dependent pKA calculations provide evidence that Lys362 may protonate transiently, thereby opening the K-channel only in the reductive second half of the reaction cycle. From our results, we develop a model that assigns a key role to Lys362 in the proton gating between the two proton input channels of the A-type CeO.

Conceptual Design and Analysis of Cold Mass Support of the CS3U Feeder for the ITER

NASA Astrophysics Data System (ADS)

Zhu, Yinfeng; Song, Yuntao; Zhang, Yuanbin; Wang, Zhongwei

2013-06-01

In the International Thermonuclear Experimental Reactor (ITER) project, the feeders are one of the most important and critical systems. To convey the power supply and the coolant for the central solenoid (CS) magnet, 6 sets of CS feeders are employed, which consist mainly of an in-cryostat feeder (ICF), a cryostat feed-through (CFT), an S-bend box (SBB), and a coil terminal box (CTB). To compensate the displacements of the internal components of the CS feeders during operation, sliding cold mass supports consisting of a sled plate, a cylindrical support, a thermal shield, and an external ring are developed. To check the strength of the developed cold mass supports of the CS3U feeder, electromagnetic analysis of the two superconducting busbars is performed by using the CATIA V5 and ANSYS codes based on parametric technology. Furthermore, the thermal-structural coupling analysis is performed based on the obtained results, except for the stress concentration, and the max. stress intensity is lower than the allowable stress of the selected material. It is found that the conceptual design of the cold mass support can satisfy the required functions under the worst case of normal working conditions. All these performed activities will provide a firm technical basis for the engineering design and development of cold mass supports.

Wireless acoustic-electric feed-through for power and signal transmission

NASA Technical Reports Server (NTRS)

Doty, Benjamin (Inventor); Badescu, Mircea (Inventor); Sherrit, Stewart (Inventor); Bao, Xiaoqi (Inventor); Bar-Cohen, Yoseph (Inventor); Chang, Zensheu (Inventor)

2011-01-01

An embodiment provides electrical energy from a source on one side of a medium to a load on the other side of the medium, the embodiment including a first piezoelectric to generate acoustic energy in response to electrical energy from the source, and a second piezoelectric to convert the received acoustic energy to electrical energy used by the load. Other embodiments are described and claimed.

MEMS for Practical Applications

NASA Astrophysics Data System (ADS)

Esashi, Masayoshi

Silicon MEMS as electrostatically levitated rotational gyroscopes and 2D optical scanners, and wafer level packaged devices as integrated capacitive pressure sensors and MEMS switches are described. MEMS which use non-silicon materials as LTCC with electrical feedthrough, SiC and LiNbO3 for probe cards for wafer-level burn-in test, molds for glass press molding and SAW wireless passive sensors respectively are also described.

Embedding Optical Fibers In Cast Metal Parts

NASA Technical Reports Server (NTRS)

Gibler, William N.; Atkins, Robert A.; Lee, Chung E.; Taylor, Henry F.

1995-01-01

Use of metal strain reliefs eliminates breakage of fibers during casting process. Technique for embedding fused silica optical fibers in cast metal parts devised. Optical fiber embedded in flange, fitting, or wall of vacuum or pressure chamber, to provide hermetically sealed feedthrough for optical transmission of measurement or control signals. Another example, optical-fiber temperature sensor embedded in metal structural component to measure strain or temperature inside component.

Rf Feedback free electron laser

DOEpatents

Brau, Charles A.; Swenson, Donald A.; Boyd, Jr., Thomas J.

1981-01-01

A free electron laser system and electron beam system for a free electron laser which use rf feedback to enhance efficiency. Rf energy is extracted from an electron beam by decelerating cavities and returned to accelerating cavities using rf returns such as rf waveguides, rf feedthroughs, etc. This rf energy is added to rf klystron energy to lower the required input energy and thereby enhance energy efficiency of the system.

Microfabricated Nickel Based Sensors for Hostile and High Pressure Environments

NASA Astrophysics Data System (ADS)

Holt, Christopher Michael Bjustrom

This thesis outlines the development of two platforms for integrating microfabricated sensors with high pressure feedthroughs for application in hostile high temperature high pressure environments. An application in oil well production logging is explored and two sensors were implemented with these platforms for application in an oil well. The first platform developed involved microfabrication directly onto a cut and polished high pressure feedthrough. This technique enables a system that is more robust than the wire bonded silicon die technique used for MEMS integration in pressure sensors. Removing wire bonds from the traditional MEMS package allows for direct interface of a microfabricated sensor with a hostile high pressure fluid environment which is not currently possible. During the development of this platform key performance metrics included pressure testing to 70MPa and temperature cycling from 20°C to 200°C. This platform enables electronics integration with a variety of microfabricated electrical and thermal based sensors which can be immersed within the oil well environment. The second platform enabled free space fabrication of nickel microfabricated devices onto an array of pins using a thick tin sacrificial layer. This technique allowed microfabrication of metal MEMS that are released by distances of 1cm from their substrate. This method is quite flexible and allows for fabrication to be done on any pin array substrate regardless of surface quality. Being able to place released MEMS sensors directly onto traditional style circuit boards, ceramic circuit boards, electrical connectors, ribbon cables, pin headers, or high pressure feedthroughs greatly improves the variety of possible applications and reduces fabrication costs. These two platforms were then used to fabricate thermal conductivity sensors that showed excellent performance for distinguishing between oil, water, and gas phases. Testing was conducted at various flow rates and performance of the released platform was shown to be better than the performance seen in the anchored sensors while both platforms were significantly better than a simply fabricated wrapped wire sensor. The anchored platform was also used to demonstrate a traditional capacitance based fluid dielectric sensor which was found to work similarly to conventional commercial capacitance probes while being significantly smaller in size.

Ultra high speed image processing techniques. [electronic packaging techniques

NASA Technical Reports Server (NTRS)

Anthony, T.; Hoeschele, D. F.; Connery, R.; Ehland, J.; Billings, J.

1981-01-01

Packaging techniques for ultra high speed image processing were developed. These techniques involve the development of a signal feedthrough technique through LSI/VLSI sapphire substrates. This allows the stacking of LSI/VLSI circuit substrates in a 3 dimensional package with greatly reduced length of interconnecting lines between the LSI/VLSI circuits. The reduced parasitic capacitances results in higher LSI/VLSI computational speeds at significantly reduced power consumption levels.

Rf feedback free electron laser

DOEpatents

Brau, C.A.; Swenson, D.A.; Boyd, T.J. Jr.

1979-11-02

A free electron laser system and electron beam system for a free electron laser are provided which use rf feedback to enhance efficiency. Rf energy is extracted from an electron beam by decelerating cavities and returned to accelerating cavities using rf returns such as rf waveguides, rf feedthroughs, etc. This rf energy is added to rf klystron energy to lower the required input energy and thereby enhance energy efficiency of the system.

A low feed-through 3D vacuum packaging technique with silicon vias for RF MEMS resonators

NASA Astrophysics Data System (ADS)

Zhao, Jicong; Yuan, Quan; Kan, Xiao; Yang, Jinling; Yang, Fuhua

2017-01-01

This paper presents a wafer-level three-dimensional (3D) vacuum packaging technique for radio frequency microelectromechanical systems (RF MEMS) resonators. A Sn-rich Au-Sn solder bonding is employed to provide a vacuum encapsulation as well as electrical conductions. Vertical silicon vias are micro-fabricated by glass reflow process. The optimized grounding, via pitch, and all-round shielding effectively reduce feed-through capacitance. Thus the signal-to-background ratios (SBRs) of the transmission signals increase from 17 dB to 20 dB, and the quality factor (Q) values of the packaged resonators go from around 8000 up to more than 9500. The measured average leak rate and shear strength are (2.55  ±  0.9)  ×  10-8 atm-cc s-1 and 42.53  ±  4.19 MPa, respectively. Furthermore, thermal cycling test between  -40 °C and 100 °C and high temperature storage test at 150 °C show that the resonant-frequency drifts are less than  ±7 ppm. In addition, the SBRs and the Q values have no obvious change after the tests. The experimental results demonstrated that the proposed encapsulation technique is well suited for the applications of RF MEMS devices.

Purification and Characterization of EDTA Monooxygenase from the EDTA-Degrading Bacterium BNC1

PubMed Central

Payne, Jason W.; Bolton, Harvey; Campbell, James A.; Xun, Luying

1998-01-01

The synthetic chelating agent EDTA can mobilize radionuclides and heavy metals in the environment. Biodegradation of EDTA should reduce this mobilization. Although several bacteria have been reported to mineralize EDTA, little is known about the biochemistry of EDTA degradation. Understanding the biochemistry will facilitate the removal of EDTA from the environment. EDTA-degrading activities were detected in cell extracts of bacterium BNC1 when flavin mononucleotide (FMN), NADH, and O2 were present. The degradative enzyme system was separated into two different enzymes, EDTA monooxygenase and an FMN reductase. EDTA monooxygenase oxidized EDTA to glyoxylate and ethylenediaminetriacetate (ED3A), with the coconsumption of FMNH2 and O2. The FMN reductase provided EDTA monooxygenase with FMNH2 by reducing FMN with NADH. The FMN reductase was successfully substituted in the assay mixture by other FMN reductases. EDTA monooxygenase was purified to greater than 95% homogeneity and had a single polypeptide with a molecular weight of 45,000. The enzyme oxidized both EDTA complexed with various metal ions and uncomplexed EDTA. The optimal conditions for activity were pH 7.8 and 35°C. Kms were 34.1 μM for uncomplexed EDTA and 8.5 μM for MgEDTA2−; this difference in Km indicates that the enzyme has greater affinity for MgEDTA2−. The enzyme also catalyzed the release of glyoxylate from nitrilotriacetate and diethylenetriaminepentaacetate. EDTA monooxygenase belongs to a small group of FMNH2-utilizing monooxygenases that attack carbon-nitrogen, carbon-sulfur, and carbon-carbon double bonds. PMID:9683478

Atomistic Interrogation of B–N Co-dopant Structures and Their Electronic Effects in Graphene

DOE PAGES

Schiros, Theanne; Nordlund, Dennis; Palova, Lucia; ...

2016-06-21

Chemical doping has been demonstrated to be an effective method for producing high-quality, large-area graphene with controlled carrier concentrations and an atomically tailored work function. Furthermore, the emergent optoelectronic properties and surface reactivity of carbon nanostructures are dictated by the microstructure of atomic dopants. Co-doping of graphene with boron and nitrogen offers the possibility to further tune the electronic properties of graphene at the atomic level, potentially creating p- and n-type domains in a single carbon sheet, opening a gap between valence and conduction bands in the 2-D semimetal. When using a suite of high-resolution synchrotron-based X-ray techniques, scanning tunnelingmore » microscopy, and density functional theory based computation we visualize and characterize B–N dopant bond structures and their electronic effects at the atomic level in single-layer graphene grown on a copper substrate. We find there is a thermodynamic driving force for B and N atoms to cluster into BNC structures in graphene, rather than randomly distribute into isolated B and N graphitic dopants, although under the present growth conditions, kinetics limit segregation of large B–N domains. We also observe that the doping effect of these BNC structures, which open a small band gap in graphene, follows the B:N ratio (B > N, p-type; B < N, n-type; B=N, neutral). We attribute this to the comparable electron-withdrawing and -donating effects, respectively, of individual graphitic B and N dopants, although local electrostatics also play a role in the work function change.« less

Three Dimensional Imaging of Helicon Wave Fields Via Magnetic Induction Probes

DTIC Science & Technology

2009-07-13

Elastomer Flange 50 The chamber is pumped by a Varian TV-300 HT turbomolecular vacuum pump with a pumping speed of 250 l/s backed by a dry scroll ... vacuum diffusion chamber with pump locations .................................................. 49 Figure 3.2. RF power delivery system...steel, 0.5 meter diameter by 1.0 meter long vacuum chamber. It has 24 access ports / flanges of varying diameter for diagnostic feed-throughs, pumping

The Deployed Warfighter Protection (DWFP) Research Program: Developing New Public Health Pesticides, Application Technologies, and Repellent Systems

DTIC Science & Technology

2013-01-01

military use. This article highlights DWFP program accomplishments achieved through its competitive grants process, exemplified by the rodent feed...accomplishments achieved through its competitive grants process exemplified by the rodent feed-through technique with insecticidal baits for controlling...Lima, Peru . The remaining 5% of the DWFP grants have been awarded to other Federal agencies such as the Centers for Disease Control and Prevention

DOE Office of Scientific and Technical Information (OSTI.GOV)

Desjardins, Tiffany; Schmidt, Derek William; Di Stefano, Carlos

These experiments are the first experiments in the Mshock campaign at the National Ignition Facility. The experiment is scheduled to be conducted on Dec. 14, 2017. The goal of the Mshock campaign is to study feedthrough dynamics of the Richtmyer- Meshkov instability in a thin layer. These dynamics will be studied in both a reshock configuration (initially) and then in a multi-shock configuration where it is planned to reshock the RM instability up to 3 times (four shocks total).

Performance Evaluation of Real-Time Precise Point Positioning Method

NASA Astrophysics Data System (ADS)

Alcay, Salih; Turgut, Muzeyyen

2017-12-01

Post-Processed Precise Point Positioning (PPP) is a well-known zero-difference positioning method which provides accurate and precise results. After the experimental tests, IGS Real Time Service (RTS) officially provided real time orbit and clock products for the GNSS community that allows real-time (RT) PPP applications. Different software packages can be used for RT-PPP. In this study, in order to evaluate the performance of RT-PPP, 3 IGS stations are used. Results, obtained by using BKG Ntrip Client (BNC) Software v2.12, are examined in terms of both accuracy and precision.

Programmable Bio-nanochip Platform: A Point-of-Care Biosensor System with the Capacity To Learn.

PubMed

McRae, Michael P; Simmons, Glennon; Wong, Jorge; McDevitt, John T

2016-07-19

The combination of point-of-care (POC) medical microdevices and machine learning has the potential transform the practice of medicine. In this area, scalable lab-on-a-chip (LOC) devices have many advantages over standard laboratory methods, including faster analysis, reduced cost, lower power consumption, and higher levels of integration and automation. Despite significant advances in LOC technologies over the years, several remaining obstacles are preventing clinical implementation and market penetration of these novel medical microdevices. Similarly, while machine learning has seen explosive growth in recent years and promises to shift the practice of medicine toward data-intensive and evidence-based decision making, its uptake has been hindered due to the lack of integration between clinical measurements and disease determinations. In this Account, we describe recent developments in the programmable bio-nanochip (p-BNC) system, a biosensor platform with the capacity for learning. The p-BNC is a "platform to digitize biology" in which small quantities of patient sample generate immunofluorescent signal on agarose bead sensors that is optically extracted and converted to antigen concentrations. The platform comprises disposable microfluidic cartridges, a portable analyzer, automated data analysis software, and intuitive mobile health interfaces. The single-use cartridges are fully integrated, self-contained microfluidic devices containing aqueous buffers conveniently embedded for POC use. A novel fluid delivery method was developed to provide accurate and repeatable flow rates via actuation of the cartridge's blister packs. A portable analyzer instrument was designed to integrate fluid delivery, optical detection, image analysis, and user interface, representing a universal system for acquiring, processing, and managing clinical data while overcoming many of the challenges facing the widespread clinical adoption of LOC technologies. We demonstrate the p-BNC's flexibility through the completion of multiplex assays within the single-use disposable cartridges for three clinical applications: prostate cancer, ovarian cancer, and acute myocardial infarction. Toward the goal of creating "sensors that learn", we have developed and describe here the Cardiac ScoreCard, a clinical decision support system for a spectrum of cardiovascular disease. The Cardiac ScoreCard approach comprises a comprehensive biomarker panel and risk factor information in a predictive model capable of assessing early risk and late-stage disease progression for heart attack and heart failure patients. These marker-driven tests have the potential to radically reduce costs, decrease wait times, and introduce new options for patients needing regular health monitoring. Further, these efforts demonstrate the clinical utility of fusing data from information-rich biomarkers and the Internet of Things (IoT) using predictive analytics to generate single-index assessments for wellness/illness status. By promoting disease prevention and personalized wellness management, tools of this nature have the potential to improve health care exponentially.

Footprint Reduction for the Acoustic Electric Feedthrough Technique

DTIC Science & Technology

2010-03-01

input current measured using a 1 Ω sense resistor . Modulation depth of the peak- to-peak input current was 2Δ ~ 20...behaviour of an AEF arrangement formed using piezo -ceramic disks with diameter 38 mm and thickness 2 mm, across an aluminium plate with thickness 1.6 to 5...the 38 mm diameter piezo -ceramic disks. In an attempt to resolve this matter, the DSTO has examined an AEF system formed using disks with 10 mm

Reproducibility and Temporal Structure in Weekly Resting-State fMRI over a Period of 3.5 Years

PubMed Central

Choe, Ann S.; Jones, Craig K.; Joel, Suresh E.; Muschelli, John; Belegu, Visar; Caffo, Brian S.; Lindquist, Martin A.; van Zijl, Peter C. M.; Pekar, James J.

2015-01-01

Resting-state functional MRI (rs-fMRI) permits study of the brain’s functional networks without requiring participants to perform tasks. Robust changes in such resting state networks (RSNs) have been observed in neurologic disorders, and rs-fMRI outcome measures are candidate biomarkers for monitoring clinical trials, including trials of extended therapeutic interventions for rehabilitation of patients with chronic conditions. In this study, we aim to present a unique longitudinal dataset reporting on a healthy adult subject scanned weekly over 3.5 years and identify rs-fMRI outcome measures appropriate for clinical trials. Accordingly, we assessed the reproducibility, and characterized the temporal structure of, rs-fMRI outcome measures derived using independent component analysis (ICA). Data was compared to a 21-person dataset acquired on the same scanner in order to confirm that the values of the single-subject RSN measures were within the expected range as assessed from the multi-participant dataset. Fourteen RSNs were identified, and the inter-session reproducibility of outcome measures—network spatial map, temporal signal fluctuation magnitude, and between-network connectivity (BNC)–was high, with executive RSNs showing the highest reproducibility. Analysis of the weekly outcome measures also showed that many rs-fMRI outcome measures had a significant linear trend, annual periodicity, and persistence. Such temporal structure was most prominent in spatial map similarity, and least prominent in BNC. High reproducibility supports the candidacy of rs-fMRI outcome measures as biomarkers, but the presence of significant temporal structure needs to be taken into account when such outcome measures are considered as biomarkers for rehabilitation-style therapeutic interventions in chronic conditions. PMID:26517540

A cloning and expression analysis of pregnancy-associated glycoproteins expressed in trophoblasts of the white-tail deer placenta.

PubMed

Brandt, Gretchen A; Parks, Tina E; Killian, Gary; Ealy, Alan D; Green, Jonathan A

2007-11-01

The pregnancy-associated glycoproteins (PAGs) are placental proteins that have been cloned from swine, sheep, goats, and cattle, but never from animals within the Cervidae family. The goal of this work was to characterize PAGs in white-tailed deer. Placenta and uterine tissues were collected from pregnant does at days 85 and 90 of pregnancy. RNA from cotyledons was used to amplify deer PAGs by RT-PCR. Ten distinct cDNAs were cloned and sequenced. Some normally conserved amino acids comprising the catalytic site were found to be altered in deer PAGs 4, 5, and 8; another PAG, (PAG-9) was a splice variant that lacked exon 7. In each case, these mutations would likely preclude proteolytic activity for these proteins. A phylogenetic analysis revealed that most of the deer PAGs fell within the ancient PAG grouping. The remainder fell within the more modern (BNC-specific) PAG group. Western blotting was performed with anti-PAG antibodies and this analysis revealed that deer PAGs comprise a heterogeneous group based on different antigenicities and electrophoretic mobilities. Immunohistochemistry and in situ hybridization revealed some unique localization patterns of PAGs in the deer placentome compared to those in other ruminants. Most notably, deer PAGs 4 and 5, which according to the phylogeny, are "ancient PAGs," were expected to be present in all trophoblasts; instead, they were localized to the BNC. Although many of the PAGs identified here are very similar to those in Bovidae, some are clearly distinct in their expression pattern and probably possess functional roles unique to cervid reproduction. (c) 2007 Wiley-Liss, Inc.

Haloperidol-loaded lipid-core polymeric nanocapsules reduce DNA damage in blood and oxidative stress in liver and kidneys of rats

NASA Astrophysics Data System (ADS)

Roversi, Katiane; Benvegnú, Dalila M.; Roversi, Karine; Trevizol, Fabíola; Vey, Luciana T.; Elias, Fabiana; Fracasso, Rafael; Motta, Mariana H.; Ribeiro, Roseane F.; dos S. Hausen, Bruna; Moresco, Rafael N.; Garcia, Solange C.; da Silva, Cristiane B.; Burger, Marilise E.

2015-04-01

Haloperidol (HP) nanoencapsulation improves therapeutic efficacy, prolongs the drug action time, and reduces its motor side effects. However, in a view of HP toxicity in organs like liver and kidneys in addition to the lack of knowledge regarding the toxicity of polymeric nanocapsules, our aim was to verify the influence of HP-nanoformulation on toxicity and oxidative stress markers in the liver and kidneys of rats, also observing the damage caused in the blood. For such, 28 adult male Wistar rats were designated in four experimental groups ( n = 7) and treated with vehicle (C group), free haloperidol suspension (FH group), blank nanocapsules suspension (B-Nc group), and haloperidol-loaded lipid-core nanocapsules suspension (H-Nc group). The nanocapsules formulation presented the size of approximately 250 nm. All suspensions were administered to the animals (0.5 mg/kg/day-i.p.) for a period of 28 days. Our results showed that FH caused damage in the liver, evidenced by increased lipid peroxidation, plasma levels of aspartate aminotransferase, and alanine aminotransferase, as well as decreased cellular integrity and vitamin C levels. In kidneys, FH treatment caused damage to a lesser extent, observed by decreased activity of δ-aminolevulinate dehydratase (ALA-D) and levels of VIT C. In addition, FH treatment was also related to a higher DNA damage index in blood. On the other hand, animals treated with H-Nc and B-Nc did not show damage in liver, kidneys, and DNA. Our study indicates that the nanoencapsulation of haloperidol was able to prevent the sub-chronic toxicity commonly observed in liver, kidneys, and DNA, thus reflecting a pharmacological superiority in relation to free drug.

FPGA-Based Networked Phasemeter for a Heterodyne Interferometer

NASA Technical Reports Server (NTRS)

Rao, Shanti

2009-01-01

A document discusses a component of a laser metrology system designed to measure displacements along the line of sight with precision on the order of a tenth the diameter of an atom. This component, the phasemeter, measures the relative phase of two electrical signals and transfers that information to a computer. Because the metrology system measures the differences between two optical paths, the phasemeter has two inputs, called measure and reference. The reference signal is nominally a perfect square wave with a 50- percent duty cycle (though only rising edges are used). As the metrology system detects motion, the difference between the reference and measure signal phases is proportional to the displacement of the motion. The phasemeter, therefore, counts the elapsed time between rising edges in the two signals, and converts the time into an estimate of phase delay. The hardware consists of a circuit board that plugs into a COTS (commercial, off-the- shelf) Spartan-III FPGA (field-programmable gate array) evaluation board. It has two BNC inputs, (reference and measure), a CMOS logic chip to buffer the inputs, and an Ethernet jack for transmitting reduced-data to a PC. Two extra BNC connectors can be attached for future expandability, such as external synchronization. Each phasemeter handles one metrology channel. A bank of six phasemeters (and two zero-crossing detector cards) with an Ethernet switch can monitor the rigid body motion of an object. This device is smaller and cheaper than existing zero-crossing phasemeters. Also, because it uses Ethernet for communication with a computer, instead of a VME bridge, it is much easier to use. The phasemeter is a key part of the Precision Deployable Apertures and Structures strategic R&D effort to design large, deployable, segmented space telescopes.

Hydrogen gas sensor and method of manufacture

DOEpatents

McKee, John M.

1991-01-01

A sensor for measuring the pressure of hydrogen gas in a nuclear reactor, and method of manufacturing the same. The sensor comprises an elongated tube of hydrogen permeable material which is connected to a pressure transducer through a feedthrough tube which passes through a wall at the boundary of the region in which hydrogen is present. The tube is pressurized and flushed with hydrogen gas at an elevated temperature during the manufacture of the sensor in order to remove all gasses other than hydrogen from the device.

Micro/nano electro mechanical systems for practical applications

NASA Astrophysics Data System (ADS)

Esashi, Masayoshi

2009-09-01

Silicon MEMS as electrostatically levitated rotational gyroscope, 2D optical scanner and wafer level packaged devices as integrated capacitive pressure sensor and MEMS switch are described. MEMS which use non-silicon materials as diamond, PZT, conductive polymer, CNT (carbon nano tube), LTCC with electrical feedthrough, SiC (silicon carbide) and LiNbO3 for multi-probe data storage, multi-column electron beam lithography system, probe card for wafer-level burn-in test, mould for glass press moulding and SAW wireless passive sensor respectively are also described.

Planar hydrodynamic instability computations and experiments with rugby-shaped hohlraums at the Omega laser

NASA Astrophysics Data System (ADS)

Vandenboomgaerde, M.; Liberatore, S.; Galmiche, D.; Casner, A.; Huser, G.; Jadaud, J. P.; Villette, B.

2008-05-01

Implosion of inertial confinement fusion (ICF) capsule is very sensitive to the growth of sphericity perturbations. The control of the feeding of such perturbations and their transport ('feedthrough') through the ablator is a key point to reach ignition. Since 2002 [1, 2], experiments have been designed and performed on the Omega laser facility in order to study these phenomena in planar geometry. A new 'rugby shaped' hohlraum was used [3, 4]. We present experimental results and comparisons with numerical simulations.

Ventilation duct with concurrent acoustic feed-forward and decentralised structural feedback active control

NASA Astrophysics Data System (ADS)

Rohlfing, J.; Gardonio, P.

2014-02-01

This paper presents theoretical and experimental work on concurrent active noise and vibration control for a ventilation duct. The active noise control system is used to reduce the air-borne noise radiated via the duct outlet whereas the active vibration control system is used to both reduce the structure-borne noise radiated by the duct wall and to minimise the structural feed-through effect that reduces the effectiveness of the active noise control system. An elemental model based on structural mobility functions and acoustic impedance functions has been developed to investigate the principal effects and limitations of feed-forward active noise control and decentralised velocity feedback vibration control. The principal simulation results have been contrasted and validated with measurements taken on a laboratory duct set-up, equipped with an active noise control system and a decentralised vibration control system. Both simulations and experimental results show that the air-borne noise radiated from the duct outlet can be significantly attenuated using the feed-forward active noise control. In the presence of structure-borne noise the performance of the active noise control system is impaired by a structure-borne feed-through effect. Also the sound radiation from the duct wall is increased. In this case, if the active noise control is combined with a concurrent active vibration control system, the sound radiation by the duct outlet is further reduced and the sound radiation from the duct wall at low frequencies reduces noticeably.

SAFT nickel hydrogen cell cycling status

NASA Technical Reports Server (NTRS)

Borthomieu, Yannick; Duquesne, Didier

1994-01-01

An overview of the NiH2 cell development is given. The NiH2 SAFT system is an electrochemical (single or dual) stack (IPV). The stack is mounted in an hydroformed Inconel 718 vessel operating at high pressure, equipped with 'rabbit ears' ceramic brazed electrical feedthroughs. The cell design is described: positive electrode, negative electrode, and stack configuration. Overviews of low earth orbit and geostationary earth orbit cyclings are provided. DPA results are also provided. The cycling and DPA results demonstrate that SAFT NiH2 is characterized by high reliability and very stable performances.

Direct metal brazing to cermet feedthroughs

DOEpatents

Not Available

1982-07-29

An improved method for brazing metallic components to a cermet surface in an alumina substrate eliminates the prior art metallized layer over the cermet via and adjoining alumina surfaces. Instead, a nickel layer is applied over the cermet surface only and metallic components are brazed directly to this nickel coated cermet surface. As a result, heretofore unachievable tensile strength joints are produced. In addition, cermet vias with their brazed metal components can be spaced more closely in the alumina substrate because of the elimination of the prior art metallized alumina surfaces.

Direct metal brazing to cermet feedthroughs

DOEpatents

Hopper, Jr., Albert C.

1984-12-18

An improved method for brazing metallic components to a cermet surface in an alumina substrate eliminates the prior art metallized layer over the cermet via and adjoining alumina surfaces. Instead, a nickel layer is applied over the cermet surface only and metallic components are brazed directly to this nickel coated cermet surface. As a result, heretofore unachievable tensile strength joints are produced. In addition, cermet vias with their brazed metal components can be spaced more closely in the alumina substrate because of the elimination of the prior art metallized alumina surfaces.

H_Hyd_Shktub_Mshock_III, JJJ, KKK (S01,S02,S03) on the National Ignition Facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Desjardins, Tiffany; Schmidt, Derek William; Di Stefano, Carlos

2017-12-15

These experiments are the first experiments in the Mshock campaign at the National Ignition Facility. The experiment is scheduled to be conducted on Dec. 14, 2017. The goal of the Mshock campaign is to study feedthrough dynamics of the Richtmyer- Meshkov instability in a thin layer. These dynamics will be studied in both a reshock configuration (initially) and then in a multi-shock configuration where it is planned to reshock the RM instability up to 3 times (four shocks total).

Semiconductor bridge (SCB) detonator

DOEpatents

Bickes, Jr., Robert W.; Grubelich, Mark C.

1999-01-01

The present invention is a low-energy detonator for high-density secondary-explosive materials initiated by a semiconductor bridge igniter that comprises a pair of electrically conductive lands connected by a semiconductor bridge. The semiconductor bridge is in operational or direct contact with the explosive material, whereby current flowing through the semiconductor bridge causes initiation of the explosive material. Header wires connected to the electrically-conductive lands and electrical feed-throughs of the header posts of explosive devices, are substantially coaxial to the direction of current flow through the SCB, i.e., substantially coaxial to the SCB length.

Kwangju, K-57, Korea. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

DTIC Science & Technology

1974-03-06

JtTHER I 5fU -17 o CLSS HOURS (L S T. SPEED Mi..EAN (KNTS) 1 -3 4 6 7. 10 11 16 17 - 21 22 - 27 28 33 34 - 40 41 47 48- 55 56 % WIND DIR. S _ _ PEED E .3...the summary consists of a bivariate percentage frequency distribution of wet-bulb depression in 17 classes spread horizontally; by 2-degree intervals...AC 4)25f. K4{bNC() ILIA K-57 3-59,A 4 .72 ALL N STATION NAME YEARS., PArF I ~R ALL-- T5 L .T. WET BULB TEMPERATURE DEPRESSION (F) I_ TOTAL TOTAL (F

Hermetic diamond capsules for biomedical implants enabled by gold active braze alloys.

PubMed

Lichter, Samantha G; Escudié, Mathilde C; Stacey, Alastair D; Ganesan, Kumaravelu; Fox, Kate; Ahnood, Arman; Apollo, Nicholas V; Kua, Dunstan C; Lee, Aaron Z; McGowan, Ceara; Saunders, Alexia L; Burns, Owen; Nayagam, David A X; Williams, Richard A; Garrett, David J; Meffin, Hamish; Prawer, Steven

2015-01-01

As the field of biomedical implants matures the functionality of implants is rapidly increasing. In the field of neural prostheses this is particularly apparent as researchers strive to build devices that interact with highly complex neural systems such as vision, hearing, touch and movement. A retinal implant, for example, is a highly complex device and the surgery, training and rehabilitation requirements involved in deploying such devices are extensive. Ideally, such devices will be implanted only once and will continue to function effectively for the lifetime of the patient. The first and most pivotal factor that determines device longevity is the encapsulation that separates the sensitive electronics of the device from the biological environment. This paper describes the realisation of a free standing device encapsulation made from diamond, the most impervious, long lasting and biochemically inert material known. A process of laser micro-machining and brazing is described detailing the fabrication of hermetic electrical feedthroughs and laser weldable seams using a 96.4% gold active braze alloy, another material renowned for biochemical longevity. Accelerated ageing of the braze alloy, feedthroughs and hermetic capsules yielded no evidence of corrosion and no loss of hermeticity. Samples of the gold braze implanted for 15 weeks, in vivo, caused minimal histopathological reaction and results were comparable to those obtained from medical grade silicone controls. The work described represents a first account of a free standing, fully functional hermetic diamond encapsulation for biomedical implants, enabled by gold active alloy brazing and laser micro-machining. Copyright © 2015 Elsevier Ltd. All rights reserved.

Development of a static bioactive stent prototype and dynamic aneurysm-on-a-chip(TM) model for the treatment of aneurysms

NASA Astrophysics Data System (ADS)

Reece, Lisa M.

Aneurysms are pockets of blood that collect outside blood vessel walls forming dilatations and leaving arterial walls very prone to rupture. Current treatments include: (1) clipping, and (2) coil embolization, including stent-assisted coiling. While these procedures can be effective, it would be advantageous to design a biologically active stent, modified with magnetic stent coatings, allowing cells to be manipulated to heal the arterial lining. Further, velocity, pressure, and wall shear stresses aid in the disease development of aneurysmal growth, but the shear force mechanisms effecting wound closure is elusive. Due to these factors, there is a definite need to cultivate a new stent device that will aid in healing an aneurysm in situ. To this end, a static bioactive stent device was synthesized. Additionally, to study aneurysm pathogenesis, a lab-on-a-chip device (a dynamic stent device) is the key to discovering the underlying mechanisms of these lesions. A first step to the reality of a true bioactive stent involves the study of cells that can be tested against the biomaterials that constitute the stent itself. The second step is to test particles/cells in a microfluidic environment. Therefore, biocompatability data was collected against PDMS, bacterial nanocellulose (BNC), and magnetic bacterial nanocellulose (MBNC). Preliminary static bioactive stents were synthesized whereby BNC was grown to cover standard nitinol stents. In an offshoot of the original research, a two-dimensional microfluidic model, the Aneurysm-on-a-ChipTM (AOC), was the logical answer to study particle flow within an aneurysm "sac" - this was the dynamic bioactive stent device. The AOC apparatus can track particles/cells when it is coupled to a particle image velocimetry software (PIV) package. The AOC fluid flow was visualized using standard microscopy techniques with commercial microparticles/cells. Movies were taken during fluid flow experiments and PIV was utilized to monitor.

Raman Study of Uncoated and p-BN/SiC-Coated Hi-Nicalon Fiber-Reinforced Celsian Matrix Composites. Part 1; Distribution and Nanostructure of Different Phases

NASA Technical Reports Server (NTRS)

Gouadec, Gwenael; Colomban, Philippe; Bansal, Narottam P.

2000-01-01

Hi-Nicalon fiber reinforced celsian matrix composites were characterized by Raman spectroscopy and imaging, using several laser wavelengths. Composite #1 is reinforced by as-received fibers while coatings of p-BN and SiC protect the fibers in composite #2. The matrix contains traces of the hexagonal phase of celsian, which is concentrated in the neighborhood of fibers in composite #1. Some free silicon was evident in the coating of composite #2 which might involve a {BN + SiC yields BNC + Si} "reaction" at the p-BN/SiC interface. Careful analysis of C-C peaks revealed no abnormal degradation of the fiber core in the composites.

Chemical and Bandgap Engineering in Monolayer Hexagonal Boron Nitride

PubMed Central

Ba, Kun; Jiang, Wei; Cheng, Jingxin; Bao, Jingxian; Xuan, Ningning; Sun, Yangye; Liu, Bing; Xie, Aozhen; Wu, Shiwei; Sun, Zhengzong

2017-01-01

Monolayer hexagonal boron nitride (h-BN) possesses a wide bandgap of ~6 eV. Trimming down the bandgap is technically attractive, yet poses remarkable challenges in chemistry. One strategy is to topological reform the h-BN’s hexagonal structure, which involves defects or grain boundaries (GBs) engineering in the basal plane. The other way is to invite foreign atoms, such as carbon, to forge bizarre hybrid structures like hetero-junctions or semiconducting h-BNC materials. Here we successfully developed a general chemical method to synthesize these different h-BN derivatives, showcasing how the chemical structure can be manipulated with or without a graphene precursor, and the bandgap be tuned to ~2 eV, only one third of the pristine one’s. PMID:28367992

Massively parallel information processing systems for space applications

NASA Technical Reports Server (NTRS)

Schaefer, D. H.

1979-01-01

NASA is developing massively parallel systems for ultra high speed processing of digital image data collected by satellite borne instrumentation. Such systems contain thousands of processing elements. Work is underway on the design and fabrication of the 'Massively Parallel Processor', a ground computer containing 16,384 processing elements arranged in a 128 x 128 array. This computer uses existing technology. Advanced work includes the development of semiconductor chips containing thousands of feedthrough paths. Massively parallel image analog to digital conversion technology is also being developed. The goal is to provide compact computers suitable for real-time onboard processing of images.

Monopole HOMs Dumping in the LCLS-II 1.3 GHz Structure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lunin, Andrei; Khabiboulline, Timergali; Solyak, Nikolay

2017-05-01

Developing an upgrade of Linac Coherent Light Source (LCLS-II) is currently underway. The central part of LCLS-II is a continuous wave superconducting RF (CW SRF) electron linac. High order modes (HOMs) excited in SRF structures by passing beam may deteriorate beam quality and affect beam stability. In this paper we report the simulation results of monopole High Order Modes (HOM) spectrum in the 1.3 GHz accelerating structure. Optimum parameters of the HOM feedthrough are suggested for minimizing RF losses on the HOM antenna tip and for preserving an efficiency of monopole HOMs damping simultaneously.

A normal incidence, high resolution X-ray telescope for solar coronal observations

NASA Technical Reports Server (NTRS)

Golub, L.

1985-01-01

The following major activities were advanced or completed: complete design of the entire telescope assembly and fabrication of all front-end components; specification of all rocket skin sections including bulkheads, feedthroughs and access door; fabrication, curing, and delivery of the large graphite-epoxy telescope tube; engineering analysis of the primary mirror vibration test was completed and a decision made to redesign the mirror attachment to a kinematic three-point mount; detail design of the camera control, payload and housekeeping electronics; and multilayer mirror flats with 2d spacings of 50 A and 60 A.

Optimal integral force feedback for active vibration control

NASA Astrophysics Data System (ADS)

Teo, Yik R.; Fleming, Andrew J.

2015-11-01

This paper proposes an improvement to Integral Force Feedback (IFF), which is a popular method for active vibration control of structures and mechanical systems. Benefits of IFF include robustness, guaranteed stability and simplicity. However, the maximum damping performance is dependent on the stiffness of the system; hence, some systems cannot be adequately controlled. In this paper, an improvement to the classical force feedback control scheme is proposed. The improved method achieves arbitrary damping for any mechanical system by introducing a feed-through term. The proposed improvement is experimentally demonstrated by actively damping an objective lens assembly for a high-speed confocal microscope.

Semiconductor bridge (SCB) detonator

DOEpatents

Bickes, R.W. Jr.; Grubelich, M.C.

1999-01-19

The present invention is a low-energy detonator for high-density secondary-explosive materials initiated by a semiconductor bridge (SCB) igniter that comprises a pair of electrically conductive lands connected by a semiconductor bridge. The semiconductor bridge is in operational or direct contact with the explosive material, whereby current flowing through the semiconductor bridge causes initiation of the explosive material. Header wires connected to the electrically-conductive lands and electrical feed-throughs of the header posts of explosive devices, are substantially coaxial to the direction of current flow through the SCB, i.e., substantially coaxial to the SCB length. 3 figs.

The response of Galileo aft cover components to laser radiation

NASA Technical Reports Server (NTRS)

Metzger, J. W.

1982-01-01

The aft region of the Galileo probe will be subjected to severe heat transfer rates dominated by the radiation contributions. To assess the response of several vehicle aft region components to thermal radiation, tests employing a 10 KW CO2 laser were conducted. The experiments evaluated the annulus/aft cover interface, the umbilical feedthrough assembly and the mortar cover seal assembly. Experimental evidence of the response of the phenolic nylon heatshield and quantitative measures of its effect on gap geometries of several vehicle components were acquired. In addition, qualitative measures of the survivability of the irradiated components were obtained.

High-voltage R-F feedthrough bushing

DOEpatents

Grotz, G.F.

1982-09-03

Described is a multi-element, high voltage radio frequency bushing for transmitting rf energy to an antenna located in a vacuum container. The bushing includes a center conductor of complex geometrical shape, an outer coaxial shield conductor, and a thin-walled hollow truncated cone insulator disposed between central and outer conductors. The shape of the center conductor, which includes a reverse curvature portion formed of a radially inwardly directed shoulder and a convex portion, controls the uniformity of the axial surface gradient on the insulator cone. The outer shield has a first substantially cylindrical portion and a second radially inwardly extending truncated cone portion.

High voltage RF feedthrough bushing

DOEpatents

Grotz, Glenn F.

1984-01-01

Described is a multi-element, high voltage radio frequency bushing for trmitting RF energy to an antenna located in a vacuum container. The bushing includes a center conductor of complex geometrical shape, an outer coaxial shield conductor, and a thin-walled hollow truncated cone insulator disposed between central and outer conductors. The shape of the center conductor, which includes a reverse curvature portion formed of a radially inwardly directed shoulder and a convex portion, controls the uniformity of the axial surface gradient on the insulator cone. The outer shield has a first substantially cylindrical portion and a second radially inwardly extending truncated cone portion.

Development of a High Performance, Low-Profile Translation Table with Wire Feedthrough

NASA Technical Reports Server (NTRS)

Few, Alex

2016-01-01

NEAScout, a 6U cubesat, will use an 85 sq m solar sail to travel to a near-earth asteroid for observation. Over the course of the 3-year mission, a combination of reaction wheels, cold gas reaction control system, and a slow rotisserie roll about the solar sail's normal axis were expected to handle attitude control and adjust for imperfections in the deployed sail. As the design for NEAScout matured, one of the critical design parameters, the offset in the center of mass and center of pressure (CP/CM offset), proved to be sub-optimal. After significant mission and control analysis, the CP/CM offset was addressed and a new subsystem was introduced to NEAScout. This system, called the Active Mass Translator (AMT), would reside near the geometric center of NEAScout and adjust the CM by moving one portion of the flight system relative to the other. The AMT was given limited design space-about 17 mm of the vehicle's assembly height-and was required to generate +/-10 cm by +/-5 cm translation to sub-millimeter accuracy. Furthermore, the design must accommodate a large wire bundle of small gage, single strand wire and coax cables fed through the center of the mechanism. The bend radius, bend resistance, and the exposure to deep space environment complicates the AMT design and operation and necessitated a unique design to mitigate risks of wire bundle damage, binding, and cold-welding during operation. This paper will outline the design constraints for the AMT, discuss the methods and reasoning for design, and identify the lessons learned through the design downselect process and breadboarding for designing low-profile translation stages with feedthrough capabilities.

Disturbance accommodating control design for wind turbines using solvability conditions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Na; Wright, Alan D.; Balas, Mark J.

In this study, solvability conditions for disturbance accommodating control (DAC) have been discussed and applied on wind turbine controller design in above-rated wind speed to regulate rotor speed and to mitigate turbine structural loads. DAC incorporates a predetermined waveform model and uses it as part of the state-space formulation, which is known as the internal model principle to reduce or minimize the wind disturbance effects on the outputs of the wind turbine. An asymptotically stabilizing DAC controller with disturbance impact on the wind turbine being totally canceled out can be found if certain conditions are fulfilled. Designing a rotor speedmore » regulation controller without steady-state error is important for applying linear control methodology such as DAC on wind turbines. Therefore, solvability conditions of DAC without steady-state error are attractive and can be taken as examples when designing a multitask turbine controller. DAC controllers solved via Moore-Penrose Pseudoinverse and the Kronecker product are discussed, and solvability conditions of using them are given. Additionally, a new solvability condition based on inverting the feed-through D term is proposed for the sake of reducing computational burden in the Kronecker product. Applications of designing collective pitch and independent pitch controllers based on DAC are presented. Recommendations of designing a DAC-based wind turbine controller are given. A DAC controller motivated by the proposed solvability condition that utilizes the inverse of feed-through D term is developed to mitigate the blade flapwise once-per-revolution bending moment together with a standard proportional integral controller in the control loop to assist rotor speed regulation. Simulation studies verify the discussed solvability conditions of DAC and show the effectiveness of the proposed DAC control design methodology.« less

A High Power Helicon Antenna Design for DIII-D

DOE PAGES

Nagy, A.; deGrassie, J.; Moeller, C.; ...

2017-08-02

A new antenna design for driving current in high beta tokamaks using electromagnetic waves, called Helicons, will be experimentally tested for the first time at power approaching 1 megawatt (MW) in the DIII-D Tokamak. This method is expected to be more efficient than current drive using electron cyclotron waves or neutral beam injection, and may be well suited to reactor-like configurations. A low power (100 watt (W)) 476 megahertz (MHz) “comb-line” antenna, consisting of 12 inductively coupled electrostatically shielded, modular resonators, was tested in DIII-D and showed strong coupling to the plasma without disturbing its characteristics or introducing metal impurities.more » The high power antenna consists of 30 modules affixed to back-plates and mounted on the outer wall of the vacuum vessel above the mid-plane. The antenna design follows a similar low power antenna design modified to minimize RF loss. Heat removal is provided by water cooling and a novel heat conducting path using pyrolytic graphite sheet. The CuCrZr antenna modules are designed to handle high eddy current forces. The modules use molybdenum Faraday shields that have the plasma side coated with boron carbide to enhance thermal resistance and minimize high Z impurities. A RF strip-line feed routes the RF power from coaxial vacuum feed-throughs to the antenna. Multipactor analysis of the antenna, strip line, and feedthrough will be performed. A 1.2 MW, 476 MHz klystron system, provided by the Stanford Linear Accelerator (SLAC) will provide RF power to the new antenna. Lastly, a description of the design of the high power antenna, the RF strip-line feeds, and the vessel installation will be presented.« less

A High Power Helicon Antenna Design for DIII-D

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nagy, A.; deGrassie, J.; Moeller, C.

A new antenna design for driving current in high beta tokamaks using electromagnetic waves, called Helicons, will be experimentally tested for the first time at power approaching 1 megawatt (MW) in the DIII-D Tokamak. This method is expected to be more efficient than current drive using electron cyclotron waves or neutral beam injection, and may be well suited to reactor-like configurations. A low power (100 watt (W)) 476 megahertz (MHz) “comb-line” antenna, consisting of 12 inductively coupled electrostatically shielded, modular resonators, was tested in DIII-D and showed strong coupling to the plasma without disturbing its characteristics or introducing metal impurities.more » The high power antenna consists of 30 modules affixed to back-plates and mounted on the outer wall of the vacuum vessel above the mid-plane. The antenna design follows a similar low power antenna design modified to minimize RF loss. Heat removal is provided by water cooling and a novel heat conducting path using pyrolytic graphite sheet. The CuCrZr antenna modules are designed to handle high eddy current forces. The modules use molybdenum Faraday shields that have the plasma side coated with boron carbide to enhance thermal resistance and minimize high Z impurities. A RF strip-line feed routes the RF power from coaxial vacuum feed-throughs to the antenna. Multipactor analysis of the antenna, strip line, and feedthrough will be performed. A 1.2 MW, 476 MHz klystron system, provided by the Stanford Linear Accelerator (SLAC) will provide RF power to the new antenna. Lastly, a description of the design of the high power antenna, the RF strip-line feeds, and the vessel installation will be presented.« less

Deep Cryogenic Low Power 24 Bits Analog to Digital Converter with Active Reverse Cryostat

NASA Astrophysics Data System (ADS)

Turqueti, Marcos; Prestemon, Soren; Albright, Robert

LBNL is developing an innovative data acquisition module for superconductive magnets where the front-end electronics and digitizer resides inside the cryostat. This electronic package allows conventional electronic technologies such as enhanced metal-oxide-semiconductor to work inside cryostats at temperatures as low as 4.2 K. This is achieved by careful management of heat inside the module that keeps the electronic envelop at approximately 85 K. This approach avoids all the difficulties that arise from changes in carrier mobility that occur in semiconductors at deep cryogenic temperatures. There are several advantages in utilizing this system. A significant reduction in electrical noise from signals captured inside the cryostat occurs due to the low temperature that the electronics is immersed in, reducing the thermal noise. The shorter distance that signals are transmitted before digitalization reduces pickup and cross-talk between channels. This improved performance in signal-to-noise rate by itself is a significant advantage. Another important advantage is the simplification of the feedthrough interface on the cryostat head. Data coming out of the cryostat is digital and serial, dramatically reducing the number of lines going through the cryostat feedthrough interface. It is important to notice that all lines coming out of the cryostat are digital and low voltage, reducing the possibility of electric breakdown inside the cryostat. This paper will explain in details the architecture and inner workings of this data acquisition system. It will also provide the performance of the analog to digital converter when the system is immersed in liquid helium, and in liquid nitrogen. Parameters such as power dissipation, integral non-linearity, effective number of bits, signal-to-noise and distortion, will be presented for both temperatures.

Deep Cryogenic Low Power 24 Bits Analog to Digital Converter with Active Reverse Cryostat

DOE PAGES

Turqueti, Marcos; Prestemon, Soren; Albright, Robert

2015-07-15

LBNL is developing an innovative data acquisition module for superconductive magnets where the front-end electronics and digitizer resides inside the cryostat. This electronic package allows conventional electronic technologies such as enhanced metal–oxide–semiconductor to work inside cryostats at temperatures as low as 4.2 K. This is achieved by careful management of heat inside the module that keeps the electronic envelop at approximately 85 K. This approach avoids all the difficulties that arise from changes in carrier mobility that occur in semiconductors at deep cryogenic temperatures. There are several advantages in utilizing this system. A significant reduction in electrical noise from signalsmore » captured inside the cryostat occurs due to the low temperature that the electronics is immersed in, reducing the thermal noise. The shorter distance that signals are transmitted before digitalization reduces pickup and cross-talk between channels. This improved performance in signal-to-noise rate by itself is a significant advantage. Another important advantage is the simplification of the feedthrough interface on the cryostat head. Data coming out of the cryostat is digital and serial, dramatically reducing the number of lines going through the cryostat feedthrough interface. It is important to notice that all lines coming out of the cryostat are digital and low voltage, reducing the possibility of electric breakdown inside the cryostat. This paper will explain in details the architecture and inner workings of this data acquisition system. It will also provide the performance of the analog to digital converter when the system is immersed in liquid helium, and in liquid nitrogen. Parameters such as power dissipation, integral non-linearity, effective number of bits, signal-to-noise and distortion, will be presented for both temperatures.« less

Disturbance accommodating control design for wind turbines using solvability conditions

DOE PAGES

Wang, Na; Wright, Alan D.; Balas, Mark J.

2017-02-07

In this study, solvability conditions for disturbance accommodating control (DAC) have been discussed and applied on wind turbine controller design in above-rated wind speed to regulate rotor speed and to mitigate turbine structural loads. DAC incorporates a predetermined waveform model and uses it as part of the state-space formulation, which is known as the internal model principle to reduce or minimize the wind disturbance effects on the outputs of the wind turbine. An asymptotically stabilizing DAC controller with disturbance impact on the wind turbine being totally canceled out can be found if certain conditions are fulfilled. Designing a rotor speedmore » regulation controller without steady-state error is important for applying linear control methodology such as DAC on wind turbines. Therefore, solvability conditions of DAC without steady-state error are attractive and can be taken as examples when designing a multitask turbine controller. DAC controllers solved via Moore-Penrose Pseudoinverse and the Kronecker product are discussed, and solvability conditions of using them are given. Additionally, a new solvability condition based on inverting the feed-through D term is proposed for the sake of reducing computational burden in the Kronecker product. Applications of designing collective pitch and independent pitch controllers based on DAC are presented. Recommendations of designing a DAC-based wind turbine controller are given. A DAC controller motivated by the proposed solvability condition that utilizes the inverse of feed-through D term is developed to mitigate the blade flapwise once-per-revolution bending moment together with a standard proportional integral controller in the control loop to assist rotor speed regulation. Simulation studies verify the discussed solvability conditions of DAC and show the effectiveness of the proposed DAC control design methodology.« less

Acoustic Mechanical Feed-Throughs for Producing Work Across a Structure

NASA Technical Reports Server (NTRS)

Sherril, Stewart (Inventor); Bar-Cohen, Yoseph (Inventor); Bao, Xiaoqi (Inventor)

2017-01-01

An apparatus that passes vibrational energy across a mechanical structure lacking a perforation. The disclosed apparatus and method provide the ability to transfer work (rotary or linear motion) across pressure or thermal barriers or in a sterile environment without generating contaminants; the presence of reflectors in the solid barrier to enhance the efficiency of the energy/power transmission, and the ability to produce a bi-directional driving mechanism using a plurality of different mode resonances, such as a fundamental frequency resonance and a higher frequency resonance. In some instances, a plane within the mechanical structure lacking a perforation is a nodal plane of the vibrational energy field.

Reduction of CMOS Image Sensor Read Noise to Enable Photon Counting.

PubMed

Guidash, Michael; Ma, Jiaju; Vogelsang, Thomas; Endsley, Jay

2016-04-09

Recent activity in photon counting CMOS image sensors (CIS) has been directed to reduction of read noise. Many approaches and methods have been reported. This work is focused on providing sub 1 e(-) read noise by design and operation of the binary and small signal readout of photon counting CIS. Compensation of transfer gate feed-through was used to provide substantially reduced CDS time and source follower (SF) bandwidth. SF read noise was reduced by a factor of 3 with this method. This method can be applied broadly to CIS devices to reduce the read noise for small signals to enable use as a photon counting sensor.

STS-55 German payload specialists pose in front of SL-D2 module at KSC

NASA Technical Reports Server (NTRS)

1992-01-01

STS-55 Columbia, Orbiter Vehicle (OV) 102, German payload specialists pose in front of the Spacelab Deutsche 2 (SL-D2) science module at a Kennedy Space Center (KSC) processing facility. These two Germans have been assigned to support the STS-55/SL-D2 mission. They are Payload Specialist 2 Hans Schlegel (left) and Payload Specialist 1 Ulrich Walter. Walter and Schlegel are scheduled to fly aboard OV-102 for the mission, joining five NASA astronauts. Clearly visible on the SL-D2 module are the European Space Agency (ESA) insignia, the feedthrough plate, and the D2 insignia.

Method for making electro-fluidic connections in microfluidic devices

DOEpatents

Frye-Mason, Gregory C.; Martinez, David; Manginell, Ronald P.; Heller, Edwin J.; Chanchani, Rajen

2004-08-10

A method for forming electro-fluidic interconnections in microfluidic devices comprises forming an electrical connection between matching bond pads on a die containing an active electrical element and a microfluidic substrate and forming a fluidic seal ring that circumscribes the active electrical element and a fluidic feedthrough. Preferably, the electrical connection and the seal ring are formed in a single bonding step. The simple method is particularly useful for chemical microanalytical systems wherein a plurality of microanalytical components, such as a chemical preconcentrator, a gas chromatography column, and a surface acoustic wave detector, are fluidically interconnected on a hybrid microfluidic substrate having electrical connection to external support electronics.

Electrically tunable liquid crystal photonic bandgap fiber laser

NASA Astrophysics Data System (ADS)

Olausson, Christina B.; Scolari, Lara; Wei, Lei; Noordegraaf, Danny; Weirich, Johannes; Alkeskjold, Thomas T.; Hansen, Kim P.; Bjarklev, Anders

2010-02-01

We demonstrate electrical tunability of a fiber laser using a liquid crystal photonic bandgap fiber. Tuning of the laser is achieved by combining the wavelength filtering effect of a liquid crystal photonic bandgap fiber device with an ytterbium-doped photonic crystal fiber. We fabricate an all-spliced laser cavity based on a liquid crystal photonic bandgap fiber mounted on a silicon assembly, a pump/signal combiner with single-mode signal feed-through and an ytterbium-doped photonic crystal fiber. The laser cavity produces a single-mode output and is tuned in the range 1040- 1065 nm by applying an electric field to the silicon assembly.

Exploring signatures of positive selection in pigmentation candidate genes in populations of East Asian ancestry

PubMed Central

2013-01-01

Background Currently, there is very limited knowledge about the genes involved in normal pigmentation variation in East Asian populations. We carried out a genome-wide scan of signatures of positive selection using the 1000 Genomes Phase I dataset, in order to identify pigmentation genes showing putative signatures of selective sweeps in East Asia. We applied a broad range of methods to detect signatures of selection including: 1) Tests designed to identify deviations of the Site Frequency Spectrum (SFS) from neutral expectations (Tajima’s D, Fay and Wu’s H and Fu and Li’s D* and F*), 2) Tests focused on the identification of high-frequency haplotypes with extended linkage disequilibrium (iHS and Rsb) and 3) Tests based on genetic differentiation between populations (LSBL). Based on the results obtained from a genome wide analysis of 25 kb windows, we constructed an empirical distribution for each statistic across all windows, and identified pigmentation genes that are outliers in the distribution. Results Our tests identified twenty genes that are relevant for pigmentation biology. Of these, eight genes (ATRN, EDAR, KLHL7, MITF, OCA2, TH, TMEM33 and TRPM1,) were extreme outliers (top 0.1% of the empirical distribution) for at least one statistic, and twelve genes (ADAM17, BNC2, CTSD, DCT, EGFR, LYST, MC1R, MLPH, OPRM1, PDIA6, PMEL (SILV) and TYRP1) were in the top 1% of the empirical distribution for at least one statistic. Additionally, eight of these genes (BNC2, EGFR, LYST, MC1R, OCA2, OPRM1, PMEL (SILV) and TYRP1) have been associated with pigmentary traits in association studies. Conclusions We identified a number of putative pigmentation genes showing extremely unusual patterns of genetic variation in East Asia. Most of these genes are outliers for different tests and/or different populations, and have already been described in previous scans for positive selection, providing strong support to the hypothesis that recent selective sweeps left a signature in these regions. However, it will be necessary to carry out association and functional studies to demonstrate the implication of these genes in normal pigmentation variation. PMID:23848512

PGV04, an HIV-1 gp120 CD4 Binding Site Antibody, Is Broad and Potent in Neutralization but Does Not Induce Conformational Changes Characteristic of CD4

PubMed Central

Falkowska, Emilia; Ramos, Alejandra; Feng, Yu; Zhou, Tongqing; Moquin, Stephanie; Walker, Laura M.; Wu, Xueling; Seaman, Michael S.; Wrin, Terri; Kwong, Peter D.; Wyatt, Richard T.; Mascola, John R.; Poignard, Pascal

2012-01-01

Recently, several broadly neutralizing monoclonal antibodies (bnMAbs) directed to the CD4-binding site (CD4bs) of gp120 have been isolated from HIV-1-positive donors. These include VRC01, 3BNC117, and NIH45-46, all of which are capable of neutralizing about 90% of circulating HIV-1 isolates and all of which induce conformational changes in the HIV-1 gp120 monomer similar to those induced by the CD4 receptor. In this study, we characterize PGV04 (also known as VRC-PG04), a MAb with potency and breadth that rivals those of the prototypic VRC01 and 3BNC117. When screened on a large panel of viruses, the neutralizing profile of PGV04 was distinct from those of CD4, b12, and VRC01. Furthermore, the ability of PGV04 to neutralize pseudovirus containing single alanine substitutions exhibited a pattern distinct from those of the other CD4bs MAbs. In particular, substitutions D279A, I420A, and I423A were found to abrogate PGV04 neutralization. In contrast to VRC01, PGV04 did not enhance the binding of 17b or X5 to their epitopes (the CD4-induced [CD4i] site) in the coreceptor region on the gp120 monomer. Furthermore, in contrast to CD4, none of the anti-CD4bs MAbs induced the expression of the 17b epitope on cell surface-expressed cleaved Env trimers. We conclude that potent CD4bs bnMAbs can display differences in the way they recognize and access the CD4bs and that mimicry of CD4, as assessed by inducing conformational changes in monomeric gp120 that lead to enhanced exposure of the CD4i site, is not uniquely correlated with effective neutralization at the site of CD4 binding on HIV-1. PMID:22345481

Exploring signatures of positive selection in pigmentation candidate genes in populations of East Asian ancestry.

PubMed

Hider, Jessica L; Gittelman, Rachel M; Shah, Tapan; Edwards, Melissa; Rosenbloom, Arnold; Akey, Joshua M; Parra, Esteban J

2013-07-12

Currently, there is very limited knowledge about the genes involved in normal pigmentation variation in East Asian populations. We carried out a genome-wide scan of signatures of positive selection using the 1000 Genomes Phase I dataset, in order to identify pigmentation genes showing putative signatures of selective sweeps in East Asia. We applied a broad range of methods to detect signatures of selection including: 1) Tests designed to identify deviations of the Site Frequency Spectrum (SFS) from neutral expectations (Tajima's D, Fay and Wu's H and Fu and Li's D* and F*), 2) Tests focused on the identification of high-frequency haplotypes with extended linkage disequilibrium (iHS and Rsb) and 3) Tests based on genetic differentiation between populations (LSBL). Based on the results obtained from a genome wide analysis of 25 kb windows, we constructed an empirical distribution for each statistic across all windows, and identified pigmentation genes that are outliers in the distribution. Our tests identified twenty genes that are relevant for pigmentation biology. Of these, eight genes (ATRN, EDAR, KLHL7, MITF, OCA2, TH, TMEM33 and TRPM1,) were extreme outliers (top 0.1% of the empirical distribution) for at least one statistic, and twelve genes (ADAM17, BNC2, CTSD, DCT, EGFR, LYST, MC1R, MLPH, OPRM1, PDIA6, PMEL (SILV) and TYRP1) were in the top 1% of the empirical distribution for at least one statistic. Additionally, eight of these genes (BNC2, EGFR, LYST, MC1R, OCA2, OPRM1, PMEL (SILV) and TYRP1) have been associated with pigmentary traits in association studies. We identified a number of putative pigmentation genes showing extremely unusual patterns of genetic variation in East Asia. Most of these genes are outliers for different tests and/or different populations, and have already been described in previous scans for positive selection, providing strong support to the hypothesis that recent selective sweeps left a signature in these regions. However, it will be necessary to carry out association and functional studies to demonstrate the implication of these genes in normal pigmentation variation.

MCNP simulation of the dose distribution in liver cancer treatment for BNC therapy

NASA Astrophysics Data System (ADS)

Krstic, Dragana; Jovanovic, Zoran; Markovic, Vladimir; Nikezic, Dragoslav; Urosevic, Vlade

2014-10-01

The Boron Neutron Capture Therapy ( BNCT) is based on selective uptake of boron in tumour tissue compared to the surrounding normal tissue. Infusion of compounds with boron is followed by irradiation with neutrons. Neutron capture on 10B, which gives rise to an alpha particle and recoiled 7Li ion, enables the therapeutic dose to be delivered to tumour tissue while healthy tissue can be spared. Here, therapeutic abilities of BNCT were studied for possible treatment of liver cancer using thermal and epithermal neutron beam. For neutron transport MCNP software was used and doses in organs of interest in ORNL phantom were evaluated. Phantom organs were filled with voxels in order to obtain depth-dose distributions in them. The result suggests that BNCT using an epithermal neutron beam could be applied for liver cancer treatment.

Secure Wake-Up Scheme for WBANs

NASA Astrophysics Data System (ADS)

Liu, Jing-Wei; Ameen, Moshaddique Al; Kwak, Kyung-Sup

Network life time and hence device life time is one of the fundamental metrics in wireless body area networks (WBAN). To prolong it, especially those of implanted sensors, each node must conserve its energy as much as possible. While a variety of wake-up/sleep mechanisms have been proposed, the wake-up radio potentially serves as a vehicle to introduce vulnerabilities and attacks to WBAN, eventually resulting in its malfunctions. In this paper, we propose a novel secure wake-up scheme, in which a wake-up authentication code (WAC) is employed to ensure that a BAN Node (BN) is woken up by the correct BAN Network Controller (BNC) rather than unintended users or malicious attackers. The scheme is thus particularly implemented by a two-radio architecture. We show that our scheme provides higher security while consuming less energy than the existing schemes.

Ring lens focusing and push-pull tracking scheme for optical disk systems

NASA Technical Reports Server (NTRS)

Gerber, R.; Zambuto, J.; Erwin, J. K.; Mansuripur, M.

1993-01-01

An experimental comparison of the ring lens and the astigmatic techniques of generating focus-error-signal (FES) in optical disk systems reveals that the ring lens generates a FES over two times steeper than that produced by the astigmat. Partly due to this large slope and, in part, because of its diffraction-limited behavior, the ring lens scheme exhibits superior performance characteristics. In particular the undesirable signal known as 'feedthrough' (induced on the FES by track-crossings during the seek operation) is lower by a factor of six compared to that observed with the astigmatic method. The ring lens is easy to align and has reasonable tolerance for positioning errors.

Diffraction analysis and evaluation of several focus- and track-error detection schemes for magneto-optical disk systems

NASA Technical Reports Server (NTRS)

Bernacki, Bruce E.; Mansuripur, M.

1992-01-01

A commonly used tracking method on pre-grooved magneto-optical (MO) media is the push-pull technique, and the astigmatic method is a popular focus-error detection approach. These two methods are analyzed using DIFFRACT, a general-purpose scalar diffraction modeling program, to observe the effects on the error signals due to focusing lens misalignment, Seidel aberrations, and optical crosstalk (feedthrough) between the focusing and tracking servos. Using the results of the astigmatic/push-pull system as a basis for comparison, a novel focus/track-error detection technique that utilizes a ring toric lens is evaluated as well as the obscuration method (focus error detection only).

Development of a high temperature ceramic-to-metal seal for Air Force Weapons Laboratory Laser

NASA Astrophysics Data System (ADS)

Honnell, R. E.; Stoddard, S. D.

1987-03-01

Procedures were developed for fabricating vacuum tight metal-to-ceramic ring seals between Inconel 625 and MgO-3 wt % Y2O3 tubes metallized with a calcia-alumina-silica glass (CaO-29 wt % Al2O3-35 wt % SiO2) containing 50 vol % molybdenum filler. Palniro No. 1 (Au-25 wt % Pd-25 wt % Ni) was found to be the most reliable braze for joining Inconel to metallized MgO-3 wt % Y2O3 bodies. The reliabilities of the processing procedures and the material systems were demonstrated. A prototype electrical feedthrough was fabricated for 1173 K operation in air or vacuum.

A normal incidence X-ray telescope sounding rocket payload

NASA Technical Reports Server (NTRS)

Golub, L.

1985-01-01

Progress is reported on the following major activities on the X-ray telescope: (1) complete design of the entire telescope assembly and fabrication of all front-end components was completed; (2) all rocket skin sections, including bulkheads, feedthroughs and access door, were specified; (3) fabrication, curing and delivery of the large graphite-epoxy telescope tube were completed; (4) an engineering analysis of the primary mirror vibration test was completed and a decision made to redesign the mirror attachment system to a kinematic three-point mount; (5) detail design of the camera control, payload and housekeeping electronics were completed; and (6) multilayer mirror plates with 2d spacings of 50 A and 60 A were produced.

Reduction of CMOS Image Sensor Read Noise to Enable Photon Counting

PubMed Central

Guidash, Michael; Ma, Jiaju; Vogelsang, Thomas; Endsley, Jay

2016-01-01

Recent activity in photon counting CMOS image sensors (CIS) has been directed to reduction of read noise. Many approaches and methods have been reported. This work is focused on providing sub 1 e− read noise by design and operation of the binary and small signal readout of photon counting CIS. Compensation of transfer gate feed-through was used to provide substantially reduced CDS time and source follower (SF) bandwidth. SF read noise was reduced by a factor of 3 with this method. This method can be applied broadly to CIS devices to reduce the read noise for small signals to enable use as a photon counting sensor. PMID:27070625

Development of a High Performance, Low Profile Translation Table with Wire Feedthrough for a Deep Space CubeSat

NASA Technical Reports Server (NTRS)

Few, Alex

2016-01-01

NEAScout, a 6U cubesat and secondary payload on NASA's EM-1, will use an 85 sq m solar sail to travel to a near-earth asteroid at about 1 Astronomical Unit (about 1.5 x 10(exp 8) km) for observation and reconnaissance1. A combination of reaction wheels, reaction control system, and a slow rotisserie roll about the solar sail's normal axis were expected to handle attitude control and adjust for imperfections in the deployed sail during the 2.5-year mission. As the design for NEAScout matured, one of the critical design parameters, the offset in the center of mass and center of pressure (CP/CM offset), proved to be sub-optimal. After significant mission and control analysis, the CP/CM offset was accommodated by the addition of a new subsystem to NEAScout. This system, called the Active Mass Translator (AMT), would reside near the geometric center of NEAScout and adjust the CM by moving one portion of the flight system relative to the other. The AMT was given limited design space - 17 mm of the vehicle's assembly height-and was required to generate +/-8 cm by +/-2 cm translation to sub-millimeter accuracy. Furthermore, the design must accommodate a large wire bundle of small gage, single strand wire and coax cables fed through the center of the mechanism. The bend radius, bend resistance, and the exposure to deep space environment complicates the AMT design and operation and necessitated a unique design to mitigate risks of wire bundle damage, binding, and cold-welding during operation. This paper will outline the design constraints for the AMT, discuss the methods and reasoning for design, and identify the lessons learned through the designing, breadboarding and testing for the low-profile translation stages with wire feedthrough capability.

Development of a High-Performance, Low-Profile Translation Table with Wire Feedthrough for a Deep Space CubeSat

NASA Technical Reports Server (NTRS)

Few, Alex

2016-01-01

NEAScout, a 6U cubesat and secondary payload on NASA's EM-1, will use an 85 sq m solar sail to travel to a near-earth asteroid at about 1 Astronomical Unit (about 1.5 x 10(exp 8) km) for observation and reconnaissance1. A combination of reaction wheels, reaction control system, and a slow rotisserie roll about the solar sail's normal axis were expected to handle attitude control and adjust for imperfections in the deployed sail during the 2.5-year mission. As the design for NEAScout matured, one of the critical design parameters, the offset in the center of mass and center of pressure (CP/CM offset), proved to be sub-optimal. After significant mission and control analysis, the CP/CM offset was accommodated by the addition of a new subsystem to NEAScout. This system, called the Active Mass Translator (AMT), would reside near the geometric center of NEAScout and adjust the CM by moving one portion of the flight system relative to the other. The AMT was given limited design space - 17 mm of the vehicle's assembly height-and was required to generate +/-8 cm by +/-2 cm translation to sub-millimeter accuracy. Furthermore, the design must accommodate a large wire bundle of small gage, single strand wire and coax cables fed through the center of the mechanism. The bend radius, bend resistance, and the exposure to deep space environment complicates the AMT design and operation and necessitated a unique design to mitigate risks of wire bundle damage, binding, and cold-welding during operation. This paper will outline the design constraints for the AMT, discuss the methods and reasoning for design, and identify the lessons learned through the designing, breadboarding and testing for the low-profile translation stages with wire feedthrough capability.

Highly-hermetic feedthrough fiber pigtailed circular TO-can electro-optic sensor for avionics applications

NASA Astrophysics Data System (ADS)

Lauzon, Jocelyn; Leduc, Lorrain; Bessette, Daniel; Bélanger, Nicolas

2012-06-01

Electro-optic sensors made of lasers or photodetectors assemblies can be associated with a window interface. In order to use these sensors in an avionics application, this interface has to be set on the periphery of the aircraft. This creates constraints on both the position/access of the associated electronics circuit card and the aircraft fuselage. Using an optical fiber to guide the light signal to a sensor being situated inside the aircraft where electronics circuit cards are deployed is an obvious solution that can be readily available. Fiber collimators that adapt to circular TO-can type window sensors do exist. However, they are bulky, add weight to the sensor and necessitate regular maintenance of the optical interface since both the sensor window and the collimator end-face are unprotected against contamination. Such maintenance can be complex since the access to the electronics circuit card, where the sensor is sitting, is usually difficult. This interface alignment can also be affected by vibrations and mechanical shocks, thus impacting sensor performances. As a solution to this problem, we propose a highly-hermetic feedthrough fiber pigtailed circular TO-can package. The optical element to optical fiber interface being set inside the hermetic package, there is no risk of contamination and thus, such a component does not require any maintenance. The footprint of these sensors being identical to their window counterparts, they offer drop-in replacement opportunities. Moreover, we have validated such packaged electro-optic sensors can be made to operate between -55 to 115°C, sustain 250 temperature cycles, 1500G mechanical shocks, 20Grms random vibrations without any performance degradations. Their water content is much smaller than the 0.5% limit set by MIL-STD-883, Method 1018. They have also been verified to offer a fiber pigtail strain relief resistance over 400g. Depending on the electronics elements inside these sensors, they can be made to have a MTBF over 50 000h at 100°C.

Efficient Strategies for Predictive Cell-Level Control of Lithium-Ion Batteries

NASA Astrophysics Data System (ADS)

Xavier, Marcelo A.

This dissertation introduces a set of state-space based model predictive control (MPC) algorithms tailored to a non-zero feedthrough term to account for the ohmic resistance that is inherent to the battery dynamics. MPC is herein applied to the problem of regulating cell-level measures of performance for lithium-ion batteries; the control methodologies are used first to compute a fast charging profile that respects input, output, and state constraints, i.e., input current, terminal voltage, and state of charge for an equivalent circuit model of the battery cell, and extended later to a linearized physics-based reduced-order model. The novelty of this work can summarized as follows: (1) the MPC variants are employed to a physics based reduce-order model in order to make use of the available set of internal electrochemical variables and mitigate internal mechanisms of cell degradation. (e.g., lithium plating); (2) we developed a dual-mode MPC closed-loop paradigm that suits the battery control problem with the objective of reducing computational effort by solving simpler optimization routines and guaranteeing stability; and finally (3) we developed a completely new approach of the use of a predictive control strategy where MPC is employed as a "smart sensor" for power estimation. Results are presented that show the comparative performance of the MPC algorithms for both EMC and PBROM These results highlight that dual-mode MPC can deliver optimal input current profiles by using a shorter horizon while still guaranteeing stability. Additionally, rigorous mathematical developments are presented for the development of the MPC algorithms. The use of MPC as a "smart sensor" presents it self as an appealing method for power estimation, since MPC permits a fully dynamic input profile that is able to achieve performance right at the proper constraint boundaries. Therefore, MPC is expected to produce accurate power limits for each computed sample time when compared to the Bisection method [1] which assumes constant input values over the prediction interval.

Electrophysiological and biochemical evidence that DEG/ENaC cation channels are composed of nine subunits.

PubMed

Snyder, P M; Cheng, C; Prince, L S; Rogers, J C; Welsh, M J

1998-01-09

Members of the DEG/ENaC protein family form ion channels with diverse functions. DEG/ENaC subunits associate as hetero- and homomultimers to generate channels; however the stoichiometry of these complexes is unknown. To determine the subunit stoichiometry of the human epithelial Na+ channel (hENaC), we expressed the three wild-type hENaC subunits (alpha, beta, and gamma) with subunits containing mutations that alter channel inhibition by methanethiosulfonates. The data indicate that hENaC contains three alpha, three beta, and three gamma subunits. Sucrose gradient sedimentation of alphahENaC translated in vitro, as well as alpha-, beta-, and gammahENaC coexpressed in cells, was consistent with complexes containing nine subunits. FaNaCh and BNC1, two related DEG/ENaC channels, produced complexes of similar mass. Our results suggest a novel nine-subunit stoichiometry for the DEG/ENaC family of ion channels.

Characterization of the Etna volcanic emissions through an active biomonitoring technique (moss-bags): part 2--morphological and mineralogical features.

PubMed

Calabrese, S; D'Alessandro, W

2015-01-01

Volcanic emissions were studied at Mount Etna (Italy) by using moss-bags technique. Mosses were exposed around the volcano at different distances from the active vents to evaluate the impact of volcanic emissions in the atmosphere. Morphology and mineralogy of volcanic particulate intercepted by mosses were investigated using scanning electron microscopy (SEM) equipped with energy dispersive spectrometer (EDS). Particles emitted during passive degassing activity from the two active vents, Bocca Nuova and North East Crater (BNC and NEC), were identified as silicates, sulfates and halide compounds. In addition to volcanic particles, we found evidences also of geogenic, anthropogenic and marine spray input. The study has shown the robustness of this active biomonitoring technique to collect particles, very useful in active volcanic areas characterized by continuous degassing and often not easily accessible to apply conventional sampling techniques. Copyright © 2014 Elsevier Ltd. All rights reserved.

Solder glass sealing technology for use in packaging of fiber optic sensors

NASA Astrophysics Data System (ADS)

Kreutzmann, Gerd

1990-08-01

The solder glass sealing technology is an alternative to the direct sealing method of the socalled hboptocansu. Using solder glass for the junction of glass and the metal can the temperature at about 500 °C does not destroy the optical quality of the precision glass components. The glass can also be coated with an antireflective layer and even the sealing of filterglass is possible. In cases where coupling losses can't be tolerated, the fiber has to be fed directly through the wall into the housing. Fiber feedthroughs, using solder glass for the sealing of the fiber into a metal tube, are commonly metal soldered or welded into the wall and the fiber surface is directly leading to the semiconductor.

The design improvement of horizontal stripline kicker in TPS storage ring

NASA Astrophysics Data System (ADS)

Chou, P. J.; Chan, C. K.; Chang, C. C.; Hsu, K. T.; Hu, K. H.; Kuan, C. K.; Sheng, I. C.

2017-07-01

We plan to replace the existing horizontal stripline kicker of the transverse feedback system with an improved design. Large reflected power was observed at the downstream port of stripline kicker driven by the feedback amplifier. A rapid surge of vacuum pressure was observed when we tested the high current operation in TPS storage ring in April 2016. A burned feedthrough of the horizontal stripline kicker was discovered during a maintenance shutdown. The improved design is targeted to reduce the reflection of driving power from feedback system and to reduce beam induced RF heating. This major modification of the design is described. The results of RF simulation performed with the electromagnetic code GdfidL are reported as well.

Physical and chemical controls on the critical zone

USGS Publications Warehouse

Anderson, S.P.; Von Blanckenburg, F.; White, A.F.

2007-01-01

Geochemists have long recognized a correlation between rates of physical denudation and chemical weathering. What underlies this correlation? The Critical Zone can be considered as a feed-through reactor. Downward advance of the weathering front brings unweathered rock into the reactor. Fluids are supplied through precipitation. The reactor is stirred at the top by biological and physical processes. The balance between advance of the weathering front by mechanical and chemical processes and mass loss by denudation fixes the thickness of the Critical Zone reactor. The internal structure of this reactor is controlled by physical processes that create surface area, determine flow paths, and set the residence time of material in the Critical Zone. All of these impact chemical weathering flux.

Thin Shell, High Velocity Inertial Confinement Fusion Implosions on the National Ignition Facility

NASA Astrophysics Data System (ADS)

Ma, T.; Hurricane, O. A.; Callahan, D. A.; Barrios, M. A.; Casey, D. T.; Dewald, E. L.; Dittrich, T. R.; Döppner, T.; Haan, S. W.; Hinkel, D. E.; Berzak Hopkins, L. F.; Le Pape, S.; MacPhee, A. G.; Pak, A.; Park, H.-S.; Patel, P. K.; Remington, B. A.; Robey, H. F.; Salmonson, J. D.; Springer, P. T.; Tommasini, R.; Benedetti, L. R.; Bionta, R.; Bond, E.; Bradley, D. K.; Caggiano, J.; Celliers, P.; Cerjan, C. J.; Church, J. A.; Dixit, S.; Dylla-Spears, R.; Edgell, D.; Edwards, M. J.; Field, J.; Fittinghoff, D. N.; Frenje, J. A.; Gatu Johnson, M.; Grim, G.; Guler, N.; Hatarik, R.; Herrmann, H. W.; Hsing, W. W.; Izumi, N.; Jones, O. S.; Khan, S. F.; Kilkenny, J. D.; Knauer, J.; Kohut, T.; Kozioziemski, B.; Kritcher, A.; Kyrala, G.; Landen, O. L.; MacGowan, B. J.; Mackinnon, A. J.; Meezan, N. B.; Merrill, F. E.; Moody, J. D.; Nagel, S. R.; Nikroo, A.; Parham, T.; Ralph, J. E.; Rosen, M. D.; Rygg, J. R.; Sater, J.; Sayre, D.; Schneider, M. B.; Shaughnessy, D.; Spears, B. K.; Town, R. P. J.; Volegov, P. L.; Wan, A.; Widmann, K.; Wilde, C. H.; Yeamans, C.

2015-04-01

Experiments have recently been conducted at the National Ignition Facility utilizing inertial confinement fusion capsule ablators that are 175 and 165 μ m in thickness, 10% and 15% thinner, respectively, than the nominal thickness capsule used throughout the high foot and most of the National Ignition Campaign. These three-shock, high-adiabat, high-foot implosions have demonstrated good performance, with higher velocity and better symmetry control at lower laser powers and energies than their nominal thickness ablator counterparts. Little to no hydrodynamic mix into the DT hot spot has been observed despite the higher velocities and reduced depth for possible instability feedthrough. Early results have shown good repeatability, with up to 1 /2 the neutron yield coming from α -particle self-heating.

The Ferrofluids Story

NASA Technical Reports Server (NTRS)

1993-01-01

A new Ferrofluidics exclusion seal promises improvement in controlling "fugitive emissions" -vapors that escape into the atmosphere from petroleum refining and chemical processing facilities. These are primarily volatile organic compounds, and their emissions are highly regulated by the EPA. The ferrofluid system consists of a primary mechanical seal working in tandem with a secondary seal. Ferrofluids are magnetic liquids - fluids in which microscopic metal particles have been suspended, allowing the liquid to be controlled by a magnetic force. The concept was developed in the early years of the Space program, but never used. Two Avco scientists, however, saw commercial potential in ferrofluids and formed a company. Among exclusion seal commercial applications are rotary feedthrough seals, hydrodynamic bearings and fluids for home and automotive loudspeakers. Ferrofluidics has subsidiaries throughout the world.

TIME CALIBRATED OSCILLOSCOPE SWEEP CIRCUIT

DOEpatents

Smith, V.L.; Carstensen, H.K.

1959-11-24

An improved time calibrated sweep circuit is presented, which extends the range of usefulness of conventional oscilloscopes as utilized for time calibrated display applications in accordance with U. S. Patent No. 2,832,002. Principal novelty resides in the provision of a pair of separate signal paths, each of which is phase and amplitude adjustable, to connect a high-frequency calibration oscillator to the output of a sawtooth generator also connected to the respective horizontal deflection plates of an oscilloscope cathode ray tube. The amplitude and phase of the calibration oscillator signals in the two signal paths are adjusted to balance out feedthrough currents capacitively coupled at high frequencies of the calibration oscillator from each horizontal deflection plate to the vertical plates of the cathode ray tube.

Robust energy-absorbing compensators for the ACTEX II test article

NASA Astrophysics Data System (ADS)

Blaurock, Carl A.; Miller, David W.; Nye, Ted

1995-05-01

The paper addresses the problem of satellite solar panel vibration. A multi-layer vibration control scheme is investigated using a flight test article. Key issues in the active control portion are presented in the paper. The paper discusses the primary control design drivers, which are the time variations in modal frequencies due to configuration and thermal changes. A local control design approach is investigated, but found to be unworkable due to sensor/actuator non-collocation. An alternate design process uses linear robust control techniques, by describing the modal shifts as uncertainties. Multiple modal design, alpha- shifted multiple model, and a feedthrough compensation scheme are examined. Ground and simulation tests demonstrate that the resulting controllers provide significant vibration reduction in the presence of expected system variations.

Thin shell, high velocity inertial confinement fusion implosions on the national ignition facility.

PubMed

Ma, T; Hurricane, O A; Callahan, D A; Barrios, M A; Casey, D T; Dewald, E L; Dittrich, T R; Döppner, T; Haan, S W; Hinkel, D E; Berzak Hopkins, L F; Le Pape, S; MacPhee, A G; Pak, A; Park, H-S; Patel, P K; Remington, B A; Robey, H F; Salmonson, J D; Springer, P T; Tommasini, R; Benedetti, L R; Bionta, R; Bond, E; Bradley, D K; Caggiano, J; Celliers, P; Cerjan, C J; Church, J A; Dixit, S; Dylla-Spears, R; Edgell, D; Edwards, M J; Field, J; Fittinghoff, D N; Frenje, J A; Gatu Johnson, M; Grim, G; Guler, N; Hatarik, R; Herrmann, H W; Hsing, W W; Izumi, N; Jones, O S; Khan, S F; Kilkenny, J D; Knauer, J; Kohut, T; Kozioziemski, B; Kritcher, A; Kyrala, G; Landen, O L; MacGowan, B J; Mackinnon, A J; Meezan, N B; Merrill, F E; Moody, J D; Nagel, S R; Nikroo, A; Parham, T; Ralph, J E; Rosen, M D; Rygg, J R; Sater, J; Sayre, D; Schneider, M B; Shaughnessy, D; Spears, B K; Town, R P J; Volegov, P L; Wan, A; Widmann, K; Wilde, C H; Yeamans, C

2015-04-10

Experiments have recently been conducted at the National Ignition Facility utilizing inertial confinement fusion capsule ablators that are 175 and 165  μm in thickness, 10% and 15% thinner, respectively, than the nominal thickness capsule used throughout the high foot and most of the National Ignition Campaign. These three-shock, high-adiabat, high-foot implosions have demonstrated good performance, with higher velocity and better symmetry control at lower laser powers and energies than their nominal thickness ablator counterparts. Little to no hydrodynamic mix into the DT hot spot has been observed despite the higher velocities and reduced depth for possible instability feedthrough. Early results have shown good repeatability, with up to 1/2 the neutron yield coming from α-particle self-heating.

Architecture of PAU survey camera readout electronics

NASA Astrophysics Data System (ADS)

Castilla, Javier; Cardiel-Sas, Laia; De Vicente, Juan; Illa, Joseph; Jimenez, Jorge; Maiorino, Marino; Martinez, Gustavo

2012-07-01

PAUCam is a new camera for studying the physics of the accelerating universe. The camera will consist of eighteen 2Kx4K HPK CCDs: sixteen for science and two for guiding. The camera will be installed at the prime focus of the WHT (William Herschel Telescope). In this contribution, the architecture of the readout electronics system is presented. Back- End and Front-End electronics are described. Back-End consists of clock, bias and video processing boards, mounted on Monsoon crates. The Front-End is based on patch panel boards. These boards are plugged outside the camera feed-through panel for signal distribution. Inside the camera, individual preamplifier boards plus kapton cable completes the path to connect to each CCD. The overall signal distribution and grounding scheme is shown in this paper.

Continuous flow, explosives vapor generator and sensor chamber.

PubMed

Collins, Greg E; Giordano, Braden C; Sivaprakasam, Vasanthi; Ananth, Ramagopal; Hammond, Mark; Merritt, Charles D; Tucker, John E; Malito, Michael; Eversole, Jay D; Rose-Pehrsson, Susan

2014-05-01

A novel liquid injection vapor generator (LIVG) is demonstrated that is amenable to low vapor pressure explosives, 2,4,6-trinitrotoluene and hexahydro-1,3,5-trinitro-1,3,5-triazine. The LIVG operates in a continuous manner, providing a constant and stable vapor output over a period of days and whose concentration can be extended over as much as three orders of magnitude. In addition, a large test atmosphere chamber attached to the LIVG is described, which enables the generation of a stable test atmosphere with controllable humidity and temperature. The size of the chamber allows for the complete insertion of testing instruments or arrays of materials into a uniform test atmosphere, and various electrical feedthroughs, insertion ports, and sealed doors permit simple and effective access to the sample chamber and its vapor.

Portable Hyperbaric Chamber

NASA Technical Reports Server (NTRS)

Schneider, William C. (Inventor); Locke, James P. (Inventor); DeLaFuente, Horacio (Inventor)

2001-01-01

A portable, collapsible hyperbaric chamber was developed. A toroidal inflatable skeleton provides initial structural support for the chamber, allowing the attendant and/or patient to enter the chamber. Oval hatches mate against bulkhead rings, and the hyperbaric chamber is pressurized. The hatches seal against an o-ring, and the internal pressure of the chamber provides the required pressure against the hatch to maintain an airtight seal. In the preferred embodiment, the hyperbaric chamber has an airlock to allow the attendant to enter and exit the patient chamber during treatment. Visual communication is provided through portholes in the patient and/or airlock chamber. Life monitoring and support systems are in communication with the interior of the hyperbaric chamber and/or airlock chamber through conduits and/or sealed feed-through connectors into the hyperbaric chamber.

High Voltage Design Considerations for the Electrostatic Septum for the Mu2e Beam Resonant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alvarez, Matthew L.; Jensen, C.; Morris, D.

aTwo electrostatic septa (ESS) are being designed for the slow extraction of 8GeV proton beam for the Mu2e experiment at Fermilab. Special attention is given to the high voltage components that affect the performance of the septa. The components under consideration are the high voltage (HV) feedthrough, cathode standoff (CS), and clearing electrode ceramic standoffs (CECS). Previous experience with similar HV systems at Fermilab was used to define the evaluation criteria of the design of the high voltage components. Using electric field simulation software, high E-field intensities on the components and integrated field strength along the surface of the dielectricmore » material were minimized. Here we discuss the limitations found and improvements made based on those studies.« less

Finite-thickness effects on the Rayleigh-Taylor instability in accelerated elastic solids

NASA Astrophysics Data System (ADS)

Piriz, S. A.; Piriz, A. R.; Tahir, N. A.

2017-05-01

A physical model has been developed for the linear Rayleigh-Taylor instability of a finite-thickness elastic slab laying on top of a semi-infinite ideal fluid. The model includes the nonideal effects of elasticity as boundary conditions at the top and bottom interfaces of the slab and also takes into account the finite transit time of the elastic waves across the slab thickness. For Atwood number AT=1 , the asymptotic growth rate is found to be in excellent agreement with the exact solution [Plohr and Sharp, Z. Angew. Math. Mech. 49, 786 (1998), 10.1007/s000330050121], and a physical explanation is given for the reduction of the stabilizing effectiveness of the elasticity for the thinner slabs. The feedthrough factor is also calculated.

A robot control architecture supported on contraction theory

NASA Astrophysics Data System (ADS)

Silva, Jorge; Sequeira, João; Santos, Cristina

2017-01-01

This paper proposes fundamentals for stability and success of a global system composed by a mobile robot, a real environment and a navigation architecture with time constraints. Contraction theory is a typical framework that provides tools and properties to prove the stability and convergence of the global system to a unique fixed point that identifies the mission success. A stability indicator based on the combination contraction property is developed to identify the mission success as a stability measure. The architecture is fully designed through C1 nonlinear dynamical systems and feedthrough maps, which makes it amenable for contraction analysis. Experiments in a realistic and uncontrolled environment are realised to verify if inherent perturbations of the sensory information and of the environment affect the stability and success of the global system.

CMOS micromachined capacitive cantilevers for mass sensing

NASA Astrophysics Data System (ADS)

Li, Ying-Chung; Ho, Meng-Han; Hung, Shi-Jie; Chen, Meng-Huei; S-C Lu, Michael

2006-12-01

In this paper, we present the design, fabrication and characterization of the CMOS micromachined cantilevers for mass sensing in the femtogram range. The cantilevers consisting of multiple metal and dielectric layers are fabricated after completion of a conventional CMOS process by dry etching steps. The cantilevers are electrostatically actuated to resonance by in-plane electrodes. The mechanical resonant frequency is detected capacitively with on-chip circuitry, where the modulation technique is applied to eliminate capacitive feedthrough from the driving port and to lessen the effect of flicker noise. The highest resonant frequency of the cantilevers is measured at 396.46 kHz with a quality factor of 2600 at 10 mTorr. The resonant frequency shift after deposition of a 0.1 µm SiO2 layer is 140 Hz, averaging 353 fg Hz-1.

Trigger probe for determining the orientation of the power distribution of an electron beam

DOEpatents

Elmer, John W [Danville, CA; Palmer, Todd A [Livermore, CA; Teruya, Alan T [Livermore, CA

2007-07-17

The present invention relates to a probe for determining the orientation of electron beams being profiled. To accurately time the location of an electron beam, the probe is designed to accept electrons from only a narrowly defined area. The signal produced from the probe is then used as a timing or triggering fiducial for an operably coupled data acquisition system. Such an arrangement eliminates changes in slit geometry, an additional signal feedthrough in the wall of a welding chamber and a second timing or triggering channel on a data acquisition system. As a result, the present invention improves the accuracy of the resulting data by minimizing the adverse effects of current slit triggering methods so as to accurately reconstruct electron or ion beams.

Thin Shell, High Velocity Inertial Confinement Fusion Implosions on the National Ignition Facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ma, T.; Hurricane, O. A.; Callahan, D. A.

Experiments have recently been conducted at the National Ignition Facility utilizing inertial confinement fusion capsule ablators that are 175 and 165 μm in thickness, 10% and 15% thinner, respectively, than the nominal thickness capsule used throughout the high foot and most of the National Ignition Campaign. These three-shock, high-adiabat, high-foot implosions have demonstrated good performance, with higher velocity and better symmetry control at lower laser powers and energies than their nominal thickness ablator counterparts. Little to no hydrodynamic mix into the DT hot spot has been observed despite the higher velocities and reduced depth for possible instability feedthrough. Earlier resultsmore » have shown good repeatability, with up to 1/2 the neutron yield coming from α-particle self-heating.« less

On the measurement of intensity correlations from laboratory and astronomical sources with SPADs and SNSPDs

NASA Astrophysics Data System (ADS)

Schroeder, Edward; Mauskopf, Philip; Pilyavsky, Genady; Sinclair, Adrian; Smith, Nathan; Bryan, Sean; Mani, Hamdi; Morozov, Dmitry; Berggren, Karl; Zhu, Di; Smirnov, Konstantin; Vakhtomin, Yuriy

2016-08-01

We describe the performance of detector modules containing silicon single photon avalanche photodiodes (SPADs) and superconducting nanowire single photon detectors (SNSPDs) to be used for intensity interferometry. The SPADs are mounted in fiber-coupled and free-space coupled packages. The SNSPDs are mounted in a small liquid helium cryostat coupled to single mode fiber optic cables which pass through a hermetic feed-through. The detectors are read out with microwave amplifiers and FPGA-based coincidence electronics. We present progress on measurements of intensity correlations from incoherent sources including gas-discharge lamps and stars with these detectors. From the measured laboratory performance of the correlation system, we estimate the sensitivity to intensity correlations from stars using commercial telescopes and larger existing research telescopes.

Electroporation and use of hepatitis B virus envelope L proteins as bionanocapsules.

PubMed

Yamada, Tadanori; Jung, Joohee; Seno, Masaharu; Kondo, Akihiko; Ueda, Masakazu; Tanizawa, Katsuyuki; Kuroda, Shun'ichi

2012-06-01

Hepatitis B virus (HBV) envelope L proteins, when synthesized in yeast cells, form a hollow bionanocapsule (BNC) in which genes (including large plasmids up to 40 kbp), small interfering RNA (siRNA), drugs, and proteins can be enclosed by electroporation. BNCs made from L proteins have several advantages as a delivery system: Because they display a human liver-specific receptor (the pre-S region of the L protein) on their surface, BNCs can efficiently and specifically deliver their contents to human liver-derived cells and tissues ex vivo (in cell culture) and in vivo (in a mouse xenograft model). Retargeting can be achieved simply by substituting other biorecognition molecules such as antibodies, ligands, receptors, and homing peptides for the pre-S region. In addition, BNCs have already been proven to be safe for use in humans during their development as an immunogen of hepatitis B vaccine. This protocol describes the loading of BNCs and their use in cell culture and in vivo.

Genome-wide association study identifies novel susceptibility loci for cutaneous squamous cell carcinoma.

PubMed

Chahal, Harvind S; Lin, Yuan; Ransohoff, Katherine J; Hinds, David A; Wu, Wenting; Dai, Hong-Ji; Qureshi, Abrar A; Li, Wen-Qing; Kraft, Peter; Tang, Jean Y; Han, Jiali; Sarin, Kavita Y

2016-07-18

Cutaneous squamous cell carcinoma represents the second most common cutaneous malignancy, affecting 7-11% of Caucasians in the United States. The genetic determinants of susceptibility to cutaneous squamous cell carcinoma remain largely unknown. Here we report the results of a two-stage genome-wide association study of cutaneous squamous cell carcinoma, totalling 7,404 cases and 292,076 controls. Eleven loci reached genome-wide significance (P<5 × 10(-8)) including seven previously confirmed pigmentation-related loci: MC1R, ASIP, TYR, SLC45A2, OCA2, IRF4 and BNC2. We identify an additional four susceptibility loci: 11q23.3 CADM1, a metastasis suppressor gene involved in modifying tumour interaction with cell-mediated immunity; 2p22.3; 7p21.1 AHR, the dioxin receptor involved in anti-apoptotic pathways and melanoma progression; and 9q34.3 SEC16A, a putative oncogene with roles in secretion and cellular proliferation. These susceptibility loci provide deeper insight into the pathogenesis of squamous cell carcinoma.

A 100 MV cryomodule for CW operation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Charles Reece

2005-07-10

A cryomodule designed for high-gradient CW operation has been built at Jefferson Lab. The Renascence cryomodule is the final prototype of a design for use in the 12 GeV CEBAF upgrade. The module uses eight 7-cell 1497 MHz cavities to be individually powered by 13 kW klystrons. Specifications call for providing >109 MV CW with < 250 W of dynamic heat at 2.07 K. The module incorporates a new generation of tuners and higher power input waveguides. A mixture of the new HG and LL cavity shapes are used. A new high thermal conductivity RF feedthrough has been developed andmore » used on the 32 HOM coupler probes of Renascence. The cryomodule assembly is complete. Testing is to begin late June. Design features and initial test data will be presented.« less

Elastic airtight container for the compaction of air-sensitive materials

NASA Astrophysics Data System (ADS)

Shoulders, W. Taylor; Locke, Richard; Gaume, Romain M.

2016-06-01

We report on the design and fabrication of a simple and versatile elastic canister for the compaction and hot-pressing of air-sensitive materials. This device consists of a heated double-ended floating die assembly, enclosed in a compressible stainless steel bellows that allows the action of an external hydraulic press in a uniaxial motion. The enclosure is fitted with vacuum, gas, and electrical feedthroughs to allow for atmosphere control, heating, and in situ process monitoring. The overall chamber is compact enough to be portable and transferrable into and out of a standard laboratory glovebox, thus eliminating the problem of exposing samples to ambient atmosphere during loading and unloading. Our design has been tested up to 600 °C and 7500 kg-force applied load, conditions within which transparent ceramics of anhydrous halides can be produced.

Optical memory development. Volume 3: The membrane light value page composer

NASA Technical Reports Server (NTRS)

Cosentino, L. S.; Nagle, E. M.; Stewart, W. C.

1972-01-01

The feasibility of producing a page composer for optical memory systems using thin, deformable, membrane-mirror elements as light valves was investigated. The electromechanical and optical performances of such elements were determined both analytically and experimentally. It was found that fast switching (approximately 10 microseconds), high-contrast (10 or greater), fatigue-free operation over missions of cycles, and efficient utilization of input light could be obtained with membrane light valves. Several arrays of 64 elements were made on substrates with feedthroughs, allowing access to individual elements from the backside of the substrate. Single light valves on such arrays were successfully operated with the transistors designed and produced for selection and storage at each bit location. This simulated the operation of a prototype page composer with semiconductor chips beam-lead bonded to the back of the substrate.

A low cost ion beam profile monitor

NASA Astrophysics Data System (ADS)

Godfrey, L.; Hoyes, G. G.; Pairsuwan, W.

1990-09-01

An intercepting multiwire ion beam profile monitor, of thickness 0.9 cm and active area 5 × 5 cm, has been developed for use with the low-intensity deuteron beamline at the Fast Neutron Research Facility (FNRF), Chiang Mai University. It has been used to optimise the transport of a continuous ion beam of current up to 200 μA and kinetic energy up to 140 keV. The monitor enables the determination of the two-dimensional beam profile using closely-spaced samples at 1.5 mm, and the measurement of relative beam current. The design incorporates low material and labour costs, elimination of the need for commercial vacuum feedthroughs, a minimal amount of devoted electronics with no need for preamplifiers, and permits quick insertion of the monitors, wherever needed along the beamline, with minimum disruption to neighbouring elements.

STS-55 German payload specialists and backups pose in front of SL-D2 at KSC

NASA Technical Reports Server (NTRS)

1992-01-01

STS-55 Columbia, Orbiter Vehicle (OV) 102, German payload specialists and backup (alternate) payload specialists pose in front of the Spacelab Deutsche 2 (SL-D2) science module at a Kennedy Space Center (KSC) processing facility. These four Germans have been assigned to support the STS-55/SL-D2 mission. Left to right are Payload Specialist 2 Hans Schlegel, backup Payload Specialist Dr. P. Gerhard Thiele (kneeling), Payload Specialist 1 Ulrich Walter, and backup Payload Specialist Renate Brummer. Walter and Schlegel are scheduled to fly aboard OV-102 for the mission while Brummer and Thiele will serve as alternates and fill supportive roles on the ground. Clearly visible on the SL-D2 module are the European Space Agency (ESA) insignia, the feedthrough plate, and the D2 insignia.

Micromachined electrical cauterizer

DOEpatents

Lee, Abraham P.; Krulevitch, Peter A.; Northrup, M. Allen

1999-01-01

A micromachined electrical cauterizer. Microstructures are combined with microelectrodes for highly localized electro cauterization. Using boron etch stops and surface micromachining, microneedles with very smooth surfaces are made. Micromachining also allows for precision placement of electrodes by photolithography with micron sized gaps to allow for concentrated electric fields. A microcauterizer is fabricated by bulk etching silicon to form knife edges, then parallelly placed microelectrodes with gaps as small as 5 .mu.m are patterned and aligned adjacent the knife edges to provide homeostasis while cutting tissue. While most of the microelectrode lines are electrically insulated from the atmosphere by depositing and patterning silicon dioxide on the electric feedthrough portions, a window is opened in the silicon dioxide to expose the parallel microelectrode portion. This helps reduce power loss and assist in focusing the power locally for more efficient and safer procedures.

Micromachined electrical cauterizer

DOEpatents

Lee, A.P.; Krulevitch, P.A.; Northrup, M.A.

1999-08-31

A micromachined electrical cauterizer is disclosed. Microstructures are combined with microelectrodes for highly localized electro cauterization. Using boron etch stops and surface micromachining, microneedles with very smooth surfaces are made. Micromachining also allows for precision placement of electrodes by photolithography with micron sized gaps to allow for concentrated electric fields. A microcauterizer is fabricated by bulk etching silicon to form knife edges, then parallelly placed microelectrodes with gaps as small as 5 {mu}m are patterned and aligned adjacent the knife edges to provide homeostasis while cutting tissue. While most of the microelectrode lines are electrically insulated from the atmosphere by depositing and patterning silicon dioxide on the electric feedthrough portions, a window is opened in the silicon dioxide to expose the parallel microelectrode portion. This helps reduce power loss and assist in focusing the power locally for more efficient and safer procedures. 7 figs.

A generalised optimal linear quadratic tracker with universal applications. Part 2: discrete-time systems

NASA Astrophysics Data System (ADS)

Ebrahimzadeh, Faezeh; Tsai, Jason Sheng-Hong; Chung, Min-Ching; Liao, Ying Ting; Guo, Shu-Mei; Shieh, Leang-San; Wang, Li

2017-01-01

Contrastive to Part 1, Part 2 presents a generalised optimal linear quadratic digital tracker (LQDT) with universal applications for the discrete-time (DT) systems. This includes (1) a generalised optimal LQDT design for the system with the pre-specified trajectories of the output and the control input and additionally with both the input-to-output direct-feedthrough term and known/estimated system disturbances or extra input/output signals; (2) a new optimal filter-shaped proportional plus integral state-feedback LQDT design for non-square non-minimum phase DT systems to achieve a minimum-phase-like tracking performance; (3) a new approach for computing the control zeros of the given non-square DT systems; and (4) a one-learning-epoch input-constrained iterative learning LQDT design for the repetitive DT systems.

Fiber-Optic Continuous Liquid Sensor for Cryogenic Propellant Gauging

NASA Technical Reports Server (NTRS)

Xu. Wei

2010-01-01

An innovative fiber-optic sensor has been developed for low-thrust-level settled mass gauging with measurement uncertainty <0.5 percent over cryogenic propellant tank fill levels from 2 to 98 percent. The proposed sensor uses a single optical fiber to measure liquid level and liquid distribution of cryogenic propellants. Every point of the sensing fiber is a point sensor that not only distinguishes liquid and vapor, but also measures temperature. This sensor is able to determine the physical location of each point sensor with 1-mm spatial resolution. Acting as a continuous array of numerous liquid/vapor point sensors, the truly distributed optical sensing fiber can be installed in a propellant tank in the same manner as silicon diode point sensor stripes using only a single feedthrough to connect to an optical signal interrogation unit outside the tank. Either water or liquid nitrogen levels can be measured within 1-mm spatial resolution up to a distance of 70 meters from the optical interrogation unit. This liquid-level sensing technique was also compared to the pressure gauge measurement technique in water and liquid nitrogen contained in a vertical copper pipe with a reasonable degree of accuracy. It has been demonstrated that the sensor can measure liquid levels in multiple containers containing water or liquid nitrogen with one signal interrogation unit. The liquid levels measured by the multiple fiber sensors were consistent with those virtually measured by a ruler. The sensing performance of various optical fibers has been measured, and has demonstrated that they can survive after immersion at cryogenic temperatures. The fiber strength in liquid nitrogen has also been measured. Multiple water level tests were also conducted under various actual and theoretical vibration conditions, and demonstrated that the signal-to-noise ratio under these vibration conditions, insofar as it affects measurement accuracy, is manageable and robust enough for a wide variety of spacecraft applications. A simple solution has been developed to absorb optical energy at the termination of the optical sensor, thereby avoiding any feedback to the optical interrogation unit

Carbon Nanofibers Synthesized on Selective Substrates for Nonvolatile Memory and 3D Electronics

NASA Technical Reports Server (NTRS)

Kaul, Anupama B.; Khan, Abdur R.

2011-01-01

A plasma-enhanced chemical vapor deposition (PECVD) growth technique has been developed where the choice of starting substrate was found to influence the electrical characteristics of the resulting carbon nanofiber (CNF) tubes. It has been determined that, if the tubes are grown on refractory metallic nitride substrates, then the resulting tubes formed with dc PECVD are also electrically conducting. Individual CNFs were formed by first patterning Ni catalyst islands using ebeam evaporation and liftoff. The CNFs were then synthesized using dc PECVD with C2H2:NH3 = [1:4] at 5 Torr and 700 C, and approximately equal to 200-W plasma power. Tubes were grown directly on degenerately doped silicon <100> substrates with resistivity rho approximately equal to 1-5 meterohm-centimeter, as well as NbTiN. The approximately equal to 200-nanometer thick refractory NbTiN deposited using magnetron sputtering had rho approximately equal to 113 microohm-centimeter and was also chemically compatible with CNF synthesis. The sample was then mounted on a 45 beveled Al holder, and placed inside a SEM (scanning electron microscope). A nanomanipulator probe stage was placed inside the SEM equipped with an electrical feed-through, where tungsten probes were used to make two-terminal electrical measurements with an HP 4156C parameter analyzer. The positive terminal nanoprobe was mechanically manipulated to physically contact an individual CNF grown directly on NbTiN as shown by the SEM image in the inset of figure (a), while the negative terminal was grounded to the substrate. This revealed the tube was electrically conductive, although measureable currents could not be detected until approximately equal to 6 V, after which point current increased sharply until compliance (approximately equal to 50 nA) was reached at approximately equal to 9.5 V. A native oxide on the tungsten probe tips may contribute to a tunnel barrier, which could be the reason for the suppressed transport at low biases. Currents up to approximately 100 nA could be cycled, which are likely to propagate via the tube surface, or sidewalls, rather than the body, which is shown by the I-V in figure (a). Electrical conduction via the sidewalls is a necessity for dc NEMS (nanoelectromechanical system) applications, more so than for the field emission applications of such tubes. During the tests, high conductivity was expected, because both probes were shorted to the substrate, as shown by curve 1 in the I-V characteristic in figure (b). When a tube grown on NbTiN was probed, the response was similar to the approximately equal to 100 nA and is represented by curve 2 in figure (b), which could be cycled and propagated via the tube surface or the sidewalls. However, no measureable currents for the tube grown directly on Si were observed as shown by curve 3 in figure (b), even after testing over a range of samples. This could arise from a dielectric coating on the sidewalls for tubes on Si. As a result of the directional nature of ion bombardment during dc PECVD, Si from the substrate is likely re-sputtered and possibly coats the sidewalls.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peplov, Vladimir V; Saethre, Robert B

The Spallation Neutron Source (SNS) Linac Low Energy Beam Transport (LEBT) chopper system provides fast chopping of the H- ion beam in the LEBT structure. Four identical pulsed power supplies (pulsers) create a series of 2.5 kV pulses to the four deflection electrodes floating on the focusing voltage of -50 kV. Each pulser is connected to the electrode through the network which consists of high voltage (HV) cables, a blocking capacitor, HV feed-through connectors, current-limiting resistors and transient voltage suppressors. Effective beam chopping requires minimal rise/fall time of the rectangular HV pulses on the load. In the present configuration thesemore » values are approximately 100 ns. Methods of reducing rise/fall time on the LEBT electrodes are discussed. Results of simulation and comparative measurements of the original and upgraded system on the test stand are presented. Furthermore, the effect of these changes on reliability degradation caused by arcing in the LEBT structure is discussed.« less

Temporal evolution of surface ripples on a finite plasma slab subject to the magneto-Rayleigh-Taylor instability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weis, M. R.; Zhang, P.; Lau, Y. Y., E-mail: yylau@umich.edu

2014-12-15

Using the ideal magnetohydrodynamic model, we calculate the temporal evolution of initial ripples on the boundaries of a planar plasma slab that is subjected to the magneto-Rayleigh-Taylor instability. The plasma slab consists of three regions. We assume that in each region the plasma density is constant with an arbitrary value and the magnetic field is also constant with an arbitrary magnitude and an arbitrary direction parallel to the interfaces. Thus, the instability may be driven by a combination of magnetic pressure and kinetic pressure. The general dispersion relation is derived, together with the feedthrough factor between the two interfaces. Themore » temporal evolution is constructed from the superposition of the eigenmodes. Previously established results are recovered in the various limits. Numerical examples are given on the temporal evolution of ripples on the interfaces of the finite plasma slab.« less

Orthogonally interdigitated shielded serpentine travelling wave cathode ray tube deflection structure

DOEpatents

Hagen, E.C.; Hudson, C.L.

1995-07-25

A new deflection structure which deflects a beam of charged particles, such as an electron beam, includes a serpentine set for transmitting a deflection field, and a shielding frame for housing the serpentine set. The serpentine set includes a vertical serpentine deflection element and a horizontal serpentine deflection element. These deflection elements are identical, and are interdigitatedly and orthogonally disposed relative to each other, for forming a central transmission passage, through which the electron beam passes, and is deflected by the deflection field, so as to minimize drift space signal distortion. The shielding frame includes a plurality of ground blocks, and forms an internal serpentine trough within these ground blocks, for housing the serpentine set. The deflection structure further includes a plurality of feedthrough connectors which are inserted through the shielding frame, and which are electrically connected to the serpentine set. 10 figs.

Four-to-one power combiner for 20 GHz phased array antenna using RADC MMIC phase shifters

NASA Technical Reports Server (NTRS)

1991-01-01

The design and microwave simulation of two-to-one microstrip power combiners is described. The power combiners were designed for use in a four element phase array receive antenna subarray at 20 GHz. Four test circuits are described which were designed to enable testing of the power combiner and the four element phased array antenna. Test Circuit 1 enables measurement of the two-to-one power combiner. Test Circuit 2 enables measurement of the four-to-one power combiner. Test Circuit 3 enables measurement of a four element antenna array without phase shifting MMIC's in order to characterize the power combiner with the antenna patch-to-microstrip coaxial feedthroughs. Test circuit 4 is the four element phased array antenna including the RADC MMIC phase shifters and appropriate interconnects to provide bias voltages and control phase bits.

An exact algebraic solution of the infimum in H-infinity optimization with output feedback

NASA Technical Reports Server (NTRS)

Chen, Ben M.; Saberi, Ali; Ly, Uy-Loi

1991-01-01

This paper presents a simple and noniterative procedure for the computation of the exact value of the infimum in the standard H-infinity-optimal control with output feedback. The problem formulation is general and does not place any restrictions on the direct feedthrough terms between the control input and the controlled output variables, and between the disturbance input and the measurement output variables. The method is applicable to systems that satisfy (1) the transfer function from the control input to the controlled output is right-invertible and has no invariant zeros on the j(w) axis and, (2) the transfer function from the disturbance to the measurement output is left-invertible and has no invariant zeros on the j(w) axis. A set of necessary and sufficient conditions for the solvability of H-infinity-almost disturbance decoupling problem via measurement feedback with internal stability is also given.

The reverse laser drilling of transparent materials

NASA Technical Reports Server (NTRS)

Anthony, T. R.; Lindner, P. A.

1980-01-01

Within a limited range of incident laser-beam intensities, laser drilling of a sapphire wafer initiates on the surface of the wafer where the laser beam exits and proceeds upstream in the laser beam to the surface where the laser beam enters the wafer. This reverse laser drilling is the result of the constructive interference between the laser beam and its reflected component on the exit face of the wafer. Constructive interference occurs only at the exit face of the sapphire wafer because the internally reflected laser beam suffers no phase change there. A model describing reverse laser drilling predicts the ranges of incident laser-beam intensity where no drilling, reverse laser drilling, and forward laser drilling can be expected in various materials. The application of reverse laser drilling in fabricating feed-through conductors in silicon-on-sapphire wafers for a massively parallel processer is described.

Fabrication of SOI structures with buried cavities using Si wafer direct bonding and electrochemical etch-stop

NASA Astrophysics Data System (ADS)

Chung, Gwiy-Sang

2003-10-01

This paper describes the fabrication of SOI structures with buried cavities using SDB and electrochemical etch-stop. These methods are suitable for thick membrane fabrication with accurate thickness, uniformity, and flatness. After a feed-through hole for supplied voltage and buried cavities was formed on a handle Si wafer with p-type, the handle wafer was bonded to an active Si wafer consisting of a p-type substrate with an n-type epitaxial layer corresponding to membrane thickness. The bonded pair was then thinned until electrochemical etch-stop occurred at the pn junction during electrochemical etchback. By using the SDB SOI structure with buried cavities, active membranes, which have a free standing structure with a dimension of 900×900 μm2, were fabricated. It is confirmed that the fabrication process of the SDB SOI structure with buried cavities is a powerful and versatile technology for new MEMS applications.

Increasing the dynamic range of CMOS photodiode imagers

NASA Technical Reports Server (NTRS)

Pain, Bedabrata (Inventor); Cunningham, Thomas J. (Inventor); Hancock, Bruce R. (Inventor)

2007-01-01

A multiple-step reset process and circuit for resetting a voltage stored on a photodiode of an imaging device. A first stage of the reset occurs while a source and a drain of a pixel source-follower transistor are held at ground potential and the photodiode and a gate of the pixel source-follower transistor are charged to an initial reset voltage having potential less that of a supply voltage. A second stage of the reset occurs after the initial reset voltage is stored on the photodiode and the gate of the pixel source-follower transistor and the source and drain voltages of the pixel source-follower transistor are released from ground potential thereby allowing the source and drain voltages of the pixel source-follower transistor to assume ordinary values above ground potential and resulting in a capacitive feed-through effect that increases the voltage on the photodiode to a value greater than the initial reset voltage.

A cost-effective 25-Gb/s EML TOSA using all-in-one FPCB wiring and metal optical bench.

PubMed

Han, Young-Tak; Kwon, Oh-Kee; Lee, Dong-Hun; Lee, Chul-Wook; Leem, Young-Ahn; Shin, Jang-Uk; Park, Sang-Ho; Baek, Yongsoon

2013-11-04

We present a cost-effective 25-Gb/s electro-absorption modulator integrated laser (EML) transmitter optical sub-assembly (TOSA) using all-in-one flexible printed circuit board (FPCB) wiring and a metal optical bench (MOB). For a low cost and high bandwidth TOSA, internal and external wirings and feed-through of the TOSA to transmit radio-frequency (RF) signal are configured all-in-one using the FPCB. The FPCB is extended from an exterior of the TOSA package up to an EML chip inside the package through the slit formed on a rear sidewall of the package and die-bonded on the MOB. The EML TOSA shows a modulated output power of more than 3.5 dBm and a clear eye pattern with a dynamic extinction ratio of ~8.4 dB at a data rate of 25.78 Gb/s.

Water permeation through organic materials

NASA Astrophysics Data System (ADS)

Doughty, D. H.; West, I. A.

1981-09-01

Atmospheric moisture is routinely excluded from weapon systems by the use of elastomer seals at assembly joints and electrical feedthroughs while internal moisture is minimized by relying on desiccants and on pre-dried components assembled in special low humidity assembly rooms. Published values of the water permeation coefficient for ethylene-propylene rubber and other o-ring materials are subject to some variability and the effects of aging on water permability are unknown. We have thus devised a new and extremely sensitive method for measuring moisture permeation coefficients in organic materials. This method uses dilute tritiated water as a tracer and it is approximately two orders of magnitude more sensitive than other methods. We are therefore able to make measurements on materials under STS temperature and humidity conditions. Rate data showing the approach to equilibrium and water permeability values for a variety of elastomers are presented. The test apparatus is also described.

Noniterative computation of infimum in H(infinity) optimisation for plants with invariant zeros on the j(omega)-axis

NASA Technical Reports Server (NTRS)

Chen, B. M.; Saber, A.

1993-01-01

A simple and noniterative procedure for the computation of the exact value of the infimum in the singular H(infinity)-optimization problem is presented, as a continuation of our earlier work. Our problem formulation is general and we do not place any restrictions in the finite and infinite zero structures of the system, and the direct feedthrough terms between the control input and the controlled output variables and between the disturbance input and the measurement output variables. Our method is applicable to a class of singular H(infinity)-optimization problems for which the transfer functions from the control input to the controlled output and from the disturbance input to the measurement output satisfy certain geometric conditions. In particular, the paper extends the result of earlier work by allowing these two transfer functions to have invariant zeros on the j(omega) axis.

A wafer-level vacuum package using glass-reflowed silicon through-wafer interconnection for nano/micro devices.

PubMed

Jin, Joo-Young; Yoo, Seung-Hyun; Yoo, Byung-Wook; Kim, Yong-Kweon

2012-07-01

We propose a vacuum wafer-level packaging (WLP) process using glass-reflowed silicon via for nano/micro devices (NMDs). A through-wafer interconnection (TWIn) substrate with silicon vias and reflowed glass is introduced to accomplish a vertical feed-through of device. NMDs are fabricated in the single crystal silicon (SCS) layer which is formed on the TWIn substrate by Au eutectic bonding including Cr adhesion layer. The WLPof the devices is achieved with the capping glass wafer anodically bonded to the SCS layer. In order to demonstrate the successful hermetic packaging, we fabricated the micro-Pirani gauge in the SCS layer, and packaged it in the wafer-level. The vacuum level inside the packaging was measured to be 3.1 Torr with +/- 0.12 Torr uncertainty, and the packaging leakage was not detected during 24 hour after the packaging.

Low-temperature THz time domain waveguide spectrometer with butt-coupled emitter and detector crystal.

PubMed

Qiao, W; Stephan, D; Hasselbeck, M; Liang, Q; Dekorsy, T

2012-08-27

A compact high-resolution THz time-domain waveguide spectrometer that is operated inside a cryostat is demonstrated. A THz photo-Dember emitter and a ZnTe electro-optic detection crystal are directly attached to a parallel copper-plate waveguide. This allows the THz beam to be excited and detected entirely inside the cryostat, obviating the need for THz-transparent windows or external THz mirrors. Since no external bias for the emitter is required, no electric feed-through into the cryostat is necessary. Using asynchronous optical sampling, high resolution THz spectra are obtained in the frequency range from 0.2 to 2.0 THz. The THz emission from the photo-Dember emitter and the absorption spectrum of 1,2-dicyanobenzene film are measured as a function of temperature. An absorption peak around 750 GHz of 1,2-dicyanobenzene displays a blue shift with increasing temperature.

DC partial discharge/environmental test screening of space TWTS

NASA Astrophysics Data System (ADS)

Hai, F.; Paschen, K. W.

Direct-current partial discharge/environmental tests are being conducted on traveling wave tubes (TWTs) designated for long-term space operation to screen out tubes with high voltage defects. Two types of TWTs with different external high-voltage insulation are being examined: (1) TWTs with polymeric potting, and (2) TWTs with ceramic feedthroughs. Detection of high voltage defects in the form of cracks and seprations in potted systems is enhanced by combining dc partial discharge testing with environmental (temperature and pressure) testing. These defects are usually caused by high stresses in the potting produced during temperature excursions by the difference in thermal expansion between the potting material and the confining ceramic-metal structure. Tests of all-ceramic-insulated TWTs indicate that the high voltage problem is internal to the vacuum envelope and requires both leakage and discharge measurements for diagnosis. This problem appears to be field emission from contaminated surfaces.

MEMS-Based Solid Propellant Rocket Array Thruster

NASA Astrophysics Data System (ADS)

Tanaka, Shuji; Hosokawa, Ryuichiro; Tokudome, Shin-Ichiro; Hori, Keiichi; Saito, Hirobumi; Watanabe, Masashi; Esashi, Masayoshi

The prototype of a solid propellant rocket array thruster for simple attitude control of a 10 kg class micro-spacecraft was completed and tested. The prototype has 10×10 φ0.8 mm solid propellant micro-rockets arrayed at a pitch of 1.2 mm on a 20×22 mm substrate. To realize such a dense array of micro-rockets, each ignition heater is powered from the backside of the thruster through an electrical feedthrough which passes along a propellant cylinder wall. Boron/potassium nitrate propellant (NAB) is used with/without lead rhodanide/potassium chlorate/nitrocellulose ignition aid (RK). Impulse thrust was measured by a pendulum method in air. Ignition required electric power of at least 3 4 W with RK and 4 6 W without RK. Measured impulse thrusts were from 2×10-5 Ns to 3×10-4 Ns after the calculation of compensation for air dumping.

Laser Inspection Or Soldered Connections

NASA Astrophysics Data System (ADS)

Alper, Richard I.; Traub, Alan C.

1986-07-01

A sensitive infrared detection system monitors the slight warming and cooling of a solder joint on a PWB in response to a focused laser beam pulse lasting for 30 milliseconds. Heating and cooling rates depend on the surface finish of the solder and also upon its interr.1 features. Joints which are alike show similar heating rates; defects behave differently and are flagged as showing abnormal thermal signatures Defects include surface voids, cold solder, insufficient or missing solder, residual solder flux, contamination and large subsurface voids. Solder bridges can usually be found by targeting at suspected bridge locations. Feed-through joints at DIPs and lap joints at flat-pack ICs are readily inspected by this method. By use of computer-controlled tiltable optics, access is had to the "harder to see" joints such as at leadless chip carriers and other surface mounts. Inspection rates can be up to 10 joints per second.

Temporal evolution of surface ripples on a finite plasma slab subject to the magneto-Rayleigh-Taylor instability

DOE PAGES

Weis, Matthew Robert; Zhang, Peng; Lau, Yue Ying; ...

2014-12-17

Using the ideal magnetohydrodynamic model, we calculate the temporal evolution of initial ripples on the boundaries of a planar plasma slab that is subjected to the magneto-Rayleigh-Taylor instability. The plasma slab consists of three regions. We assume that in each region the plasma density is constant with an arbitrary value and the magnetic field is also constant with an arbitrary magnitude and an arbitrary direction parallel to the interfaces. Then, the instability may be driven by a combination of magnetic pressure and kinetic pressure. Thus the general dispersion relation is derived, together with the feedthrough factor between the two interfaces.more » The temporal evolution is constructed from the superposition of the eigenmodes. Those previously established results are recovered in the various limits. Numerical examples are given on the temporal evolution of ripples on the interfaces of the finite plasma slab.« less

Refractory metals for ARPS AMTEC cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Svedberg, R.C.; Sievers, R.C.

1998-07-01

Alkali Metal Thermal-to-Electric Converter (AMTEC) cells for the Advanced Radioisotope Power Systems (ARPS) program are being developed with refractory metals and alloys as the basic structural materials. AMTEC cell efficiency increases with cell operating temperature. For space applications, long term reliability and high efficiency are essential and refractory metals were selected because of their high temperature strength, low vapor pressure, and compatibility with sodium. However, refractory metals are sensitive to oxygen, nitrogen and hydrogen contamination and refractory metal cells cannot be processed in air. Because of this sensitivity, new manufacturing and processing techniques are being developed. In addition to structuralmore » elements, development of other refractory metal components for the AMTEC cells, such as the artery and evaporator wicks, pinchoff tubes and feedthroughs are required. Changes in cell fabrication techniques and processing procedures being implemented to manufacture refractory metal cells are discussed.« less

A Convex Formulation for Magnetic Particle Imaging X-Space Reconstruction.

PubMed

Konkle, Justin J; Goodwill, Patrick W; Hensley, Daniel W; Orendorff, Ryan D; Lustig, Michael; Conolly, Steven M

2015-01-01

Magnetic Particle Imaging (mpi) is an emerging imaging modality with exceptional promise for clinical applications in rapid angiography, cell therapy tracking, cancer imaging, and inflammation imaging. Recent publications have demonstrated quantitative mpi across rat sized fields of view with x-space reconstruction methods. Critical to any medical imaging technology is the reliability and accuracy of image reconstruction. Because the average value of the mpi signal is lost during direct-feedthrough signal filtering, mpi reconstruction algorithms must recover this zero-frequency value. Prior x-space mpi recovery techniques were limited to 1d approaches which could introduce artifacts when reconstructing a 3d image. In this paper, we formulate x-space reconstruction as a 3d convex optimization problem and apply robust a priori knowledge of image smoothness and non-negativity to reduce non-physical banding and haze artifacts. We conclude with a discussion of the powerful extensibility of the presented formulation for future applications.

Solar orbiter/PHI full disk telescope entrance window mechanical mount

NASA Astrophysics Data System (ADS)

Barandiaran, J.; Zuluaga, P.; Fernandez, A. B.; Vera, I.; Garranzo, D.; Nuñez, A.; Bastide, L.; Royo, M. T.; Alvarez, A.

2017-11-01

PHI is a diffraction limited, wavelength tunable, quasi-monochromatic, and polarization sensitive imager. These capabilities are needed to infer the magnetic field and line-of-sight (LOS) velocity of the region targeted by the spacecraft (spacecraft (S/C)). PHI will consist of two telescopes: The High Resolution Telescope (HRT)[1] and the Full Disk Telescope (FDT). The HRT and the FDT will view the Sun through entrance windows located in the S/C heat shield. These windows act as heat rejecting filters with a transmission band of about 30 nm width centered on the science wavelength, such that the total transmittance (integral over the filter curve weighted with solar spectrum, including white leakage plus transmission profile of the pass band) does not exceed 4% of the total energy falling onto the window [2][3]. The HREW filter has been designed by SELEX in the framework of an ESA led technology development activity under original ESTEC contract No. 20018/06/NL/CP[4], and extensions thereof. For FDT HREW SLEX will provide the windows and it coatings. The HREW consists of two parallel-plane substrate plates (window 1 & window 2)[5] made of SUPRASIL 300 with a central thickness of 9 mm and a wedge of 30 arcsec each. These two substrates are each coated on both sides with four different coatings. These coatings and the choice of SUPRASIL help to minimize the optical absorptivity in the substrate and to radiatively decouple the HREW, which is expected to run at high temperatures during perihelion passages, from the PHI instrument cavity. The temperature distribution of the HREW is driven by two main factors: the mechanical mounting of the substrates to the feedthrough, and the radiative environment within the heat-shield/feedthrough assembly. The mechanical mount must ensure the correct integration of both suprasil substrates in its correct position and minimize the loads in windows due to thermal induced deformations and launching vibration environment. All the subsystem must survive to a launching vibration environment and fulfill optical requirements in an environmental conditions according o its position in the external part of the spacecraft with a pressure of 0.0013Pa and a temperature -163°C

Solid-State Photomultiplier with Integrated Front End Electronics

NASA Astrophysics Data System (ADS)

Christian, James; Stapels, Christopher; Johnson, Erik; Mukhopadhyay, Sharmistha; Jie Chen, Xiao; Miskimen, Rory

2009-10-01

The instrumentation cost of physics experiments has been reduced per channel, by the use of solid-state detectors, but these cost-effective techniques have not been translated to scintillation-based detectors. When considering photodetectors, the cost per channel is determined by the use of high-voltage, analog-to-digital converters, BNC cables, and any other ancillary devices. The overhead associated with device operation limits the number of channels for the detector system, while potentially limiting the scope of physics that can be explored. The PRIMEX experiment at JLab, which is being designed to measure the radiative widths of the η and η' pseudo-scalar mesons for a more comprehensive understanding of QCD at low energies, is an example where CMOS solid-state photomultipliers (SSPMs) can be implemented. The ubiquitous nature of CMOS allows for on-chip signal processing to provide front-end electronics within the detector package. We present the results of the device development for the PRIMEX calorimeter, discussing the characteristics of SSPMs, the potential cost savings, and experimental results of on-chip signal processing.

Metabolic engineering of Corynebacterium glutamicum ATCC13869 for L-valine production.

PubMed

Chen, Cheng; Li, Yanyan; Hu, Jinyu; Dong, Xunyan; Wang, Xiaoyuan

2015-05-01

In this study, an L-valine-producing strain was developed from Corynebacterium glutamicum ATCC13869 through deletion of the three genes aceE, alaT and ilvA combined with the overexpression of six genes ilvB, ilvN, ilvC, lrp1, brnF and brnE. Overexpression of lrp1 alone increased L-valine production by 16-fold. Deletion of the aceE, alaT and ilvA increased L-valine production by 44-fold. Overexpression of the six genes ilvB, ilvN, ilvC, lrp1, brnE and brnF in the triple deletion mutant WCC003 further increased L-valine production. The strain WCC003/pJYW-4-ilvBNC1-lrp1-brnFE produced 243mM L-valine in flask cultivation and 437mM (51g/L) L-valine in fed-batch fermentation and lacked detectable amino-acid byproduct such as l-alanine and l-isoleucine that are usually found in the fermentation of L-valine-producing C. glutamicum. Copyright © 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Orthogonally interdigitated shielded serpentine travelling wave cathode ray tube deflection structure

DOEpatents

Hagen, Edward C.; Hudson, Charles L.

1995-01-01

A new deflection structure (12) which deflects a beam of charged particles, uch as an electron beam (15), includes a serpentine set (20) for transmitting a deflection field, and a shielding frame (25) for housing the serpentine set (20). The serpentine set (20) includes a vertical serpentine deflection element (22) and a horizontal serpentine deflection element (24). These deflection elements (22, 24) are identical, and are interdigitatedly and orthogonally disposed relative to each other, for forming a central transmission passage (75), through which the electron beam (15) passes, and is deflected by the deflection field, so as to minimize drift space signal distortion. The shielding frame (25) includes a plurality of ground blocks (26, 28, 30, 32), and forms an internal serpentine trough (77) within these ground blocks, for housing the serpentine set (20). The deflection structure (12) further includes a plurality of feedthrough connectors (35, 37, 35I, 37I), which are inserted through the shielding frame (25), and which are electrically connected to the serpentine set (20).

Remote temperature distribution sensing using permanent magnets

DOE PAGES

Chen, Yi; Guba, Oksana; Brooks, Carlton F.; ...

2016-10-31

Remote temperature sensing is essential for applications in enclosed vessels where feedthroughs or optical access points are not possible. A unique sensing method for measuring the temperature of multiple closely-spaced points is proposed using permanent magnets and several three-axis magnetic field sensors. The magnetic field theory for multiple magnets is discussed and a solution technique is presented. Experimental calibration procedures, solution inversion considerations and methods for optimizing the magnet orientations are described in order to obtain low-noise temperature estimates. The experimental setup and the properties of permanent magnets are shown. Finally, experiments were conducted to determine the temperature of ninemore » magnets in different configurations over a temperature range of 5 to 60 degrees Celsius and for a sensor-to-magnet distance of up to 35 mm. Furthermore, to show the possible applications of this sensing system for measuring temperatures through metal walls, additional experiments were conducted inside an opaque 304 stainless steel cylinder.« less

The Ames Vertical Gun Range

NASA Technical Reports Server (NTRS)

Karcz, J. S.; Bowling, D.; Cornelison, C.; Parrish, A.; Perez, A.; Raiche, G.; Wiens, J.-P.

2016-01-01

The Ames Vertical Gun Range (AVGR) is a national facility for conducting laboratory- scale investigations of high-speed impact processes. It provides a set of light-gas, powder, and compressed gas guns capable of accelerating projectiles to speeds up to 7 km s(exp -1). The AVGR has a unique capability to vary the angle between the projectile-launch and gravity vectors between 0 and 90 deg. The target resides in a large chamber (diameter approximately 2.5 m) that can be held at vacuum or filled with an experiment-specific atmosphere. The chamber provides a number of viewing ports and feed-throughs for data, power, and fluids. Impacts are observed via high-speed digital cameras along with investigation-specific instrumentation, such as spectrometers. Use of the range is available via grant proposals through any Planetary Science Research Program element of the NASA Research Opportunities in Space and Earth Sciences (ROSES) calls. Exploratory experiments (one to two days) are additionally possible in order to develop a new proposal.

Design of the Cryostat for HT-7U Superconducting Tokamak

NASA Astrophysics Data System (ADS)

Yu, Jie; Wu, Song-tao; Song, Yun-tao; Weng, Pei-de

2002-06-01

The cryostat of HT-7U tokamak is a large vacuum vessel surrounding the entire basic machine with a cylindrical shell, a dished top and a flat bottom. The main function of HT-7U cryostat is to provide a thermal barrier between an ambient temperature test hall and a liquid helium-cooled superconducting magnet. The loads applied to the cryostat are from sources of vacuum pressure, dead weight, seismic events and electromagnetic forces originated by eddy currents. It also provides feed-through penetrations for all the connecting elements inside and outside the cryostat. The main material selected for the cryostat is stainless steel 304L. The structural analyses including buckling for the cryostat vessel under the plasma operation condition have been carried out by using a finite element code. Stress analysis results show that the maximum stress intensity was below the allowable value. In this paper, the structural analyses and design of HT-7U cryostat are emphasized.

Time delay and integration array (TDI) using charge transfer device technology. Phase 2, volume 1: Technical

NASA Technical Reports Server (NTRS)

1977-01-01

The 20x9 TDI array was developed to meet the LANDSAT Thematic Mapper Requirements. This array is based upon a self-aligned, transparent gate, buried channel process. The process features: (1) buried channel, four phase, overlapping gate CCD's for high transfer efficiency without fat zero; (2) self-aligned transistors to minimize clock feedthrough and parasitic capacitance; and (3) transparent tin oxide electrode for high quantum efficiency with front surface irradiation. The requirements placed on the array and the performance achieved are summarized. This data is the result of flat field measurements only, no imaging or dynamic target measurements were made during this program. Measurements were performed with two different test stands. The bench test equipment fabricated for this program operated at the 8 micro sec line time and employed simple sampling of the gated MOSFET output video signal. The second stand employed Correlated Doubled Sampling (CDS) and operated at 79.2 micro sec line time.

A universal sample manipulator with 50 kV negative bias

NASA Astrophysics Data System (ADS)

Kenny, M. J.; Wielunski, L. S.; Scott, M. D.; Clissold, R. A.; Stevenson, D.; Baxter, G.

1991-04-01

A manipulator incorporating a number of novel features has been built for a research ion implanter. The system is designed to enable uniform dose implantation of both planar and nonplanar surfaces by incorporating one translational and two rotational degrees of freedom. Negative target bias of up to 50 kV may be applied to the target, thus increasing the ion energy by this amount. The target chamber and all external manipulator controls are grounded. With the exception of the high voltage power supply, cable and feedthrough, all high voltage components are within the vacuum system. A secondary electron suppression cage which can be held at a negative bias of up to 60 kV relative to the chamber (i.e. 10 kV relative to the manipulator) surrounds the manipulator. Performance has been evaluated using 15N ions and nuclear reaction analysis through 15N(p,α) 12C to profile ion concentrations for dose uniformity and for ion depth at elevated target potentials.

In situ TEM Raman spectroscopy and laser-based materials modification.

PubMed

Allen, F I; Kim, E; Andresen, N C; Grigoropoulos, C P; Minor, A M

2017-07-01

We present a modular assembly that enables both in situ Raman spectroscopy and laser-based materials processing to be performed in a transmission electron microscope. The system comprises a lensed Raman probe mounted inside the microscope column in the specimen plane and a custom specimen holder with a vacuum feedthrough for a tapered optical fiber. The Raman probe incorporates both excitation and collection optics, and localized laser processing is performed using pulsed laser light delivered to the specimen via the tapered optical fiber. Precise positioning of the fiber is achieved using a nanomanipulation stage in combination with simultaneous electron-beam imaging of the tip-to-sample distance. Materials modification is monitored in real time by transmission electron microscopy. First results obtained using the assembly are presented for in situ pulsed laser ablation of MoS 2 combined with Raman spectroscopy, complimented by electron-beam diffraction and electron energy-loss spectroscopy. Copyright © 2016 Elsevier B.V. All rights reserved.

Image charge multi-role and function detectors

NASA Astrophysics Data System (ADS)

Milnes, James; Lapington, Jon S.; Jagutzki, Ottmar; Howorth, Jon

2009-06-01

The image charge technique used with microchannel plate imaging tubes provides several operational and practical benefits by serving to isolate the electronic image readout from the detector. The simple dielectric interface between detector and readout provides vacuum isolation and no vacuum electrical feed-throughs are required. Since the readout is mechanically separate from the detector, an image tube of generic design can be simply optimised for various applications by attaching it to different readout devices and electronics. We present imaging performance results using a single image tube with a variety of readout devices suited to differing applications: (a) A four electrode charge division tetra wedge anode, optimised for best spatial resolution in photon counting mode. (b) A cross delay line anode, enabling higher count rate, and the possibility of discriminating near co-incident events, and an event timing resolution of better than 1 ns. (c) A multi-anode readout connected, either to a multi-channel oscilloscope for analogue measurements of fast optical pulses, or alternately, to a multi-channel time correlated single photon counting (TCSPC) card.

First steps towards a constructal Microbial Fuel Cell.

PubMed

Lepage, Guillaume; Perrier, Gérard; Ramousse, Julien; Merlin, Gérard

2014-06-01

In order to reach real operating conditions with consequent organic charge flow, a multi-channel reactor for Microbial Fuel Cells is designed. The feed-through double chamber reactor is a two-dimensional system with four parallel channels and Reticulated Vitreous Carbon as electrodes. Based on thermodynamical calculations, the constructal-inspired distributor is optimized with the aim to reduce entropy generation along the distributing path. In the case of negligible singular pressure drops, the Hess-Murray law links the lengths and the hydraulic diameters of the successive reducing ducts leading to one given working channel. The determination of generated entropy in the channels of our constructal MFC is based on the global hydraulic resistance caused by both regular and singular pressure drops. Polarization, power and Electrochemical Impedance Spectroscopy show the robustness and the efficiency of the cell, and therefore the potential of the constructal approach. Routes towards improvements are suggested in terms of design evolutions. Copyright © 2014 Elsevier Ltd. All rights reserved.

Electromagnetic interference in cardiac rhythm management devices.

PubMed

Sweesy, Mark W; Holland, James L; Smith, Kerry W

2004-01-01

Clinicians caring for cardiac device patients with implanted pacemakers or cardioverter defibrillators (ICDs) are frequently asked questions by their patients concerning electromagnetic interference (EMI) sources and the devices. EMI may be radiated or conducted and may be present in many different forms including (but not limited to) radiofrequency waves, microwaves, ionizing radiation, acoustic radiation, static and pulsed magnetic fields, and electric currents. Manufacturers have done an exemplary job of interference protection with device features such as titanium casing, signal filtering, interference rejection circuits, feedthrough capacitors, noise reversion function, and programmable parameters. Nevertheless, EMI remains a real concern and a potential danger. Many factors influence EMI including those which the patient can regulate (eg, distance from and duration of exposure) and some the patient cannot control (eg, intensity of the EMI field, signal frequency). Potential device responses are many and range from simple temporary oversensing to permanent device damage Several of the more common EMI-generating devices and their likely effects on cardiac devices are considered in the medical, home, and daily living and work environments.

Development of an Advanced Hydraulic Fracture Mapping System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Norm Warpinski; Steve Wolhart; Larry Griffin

The project to develop an advanced hydraulic fracture mapping system consisted of both hardware and analysis components in an effort to build, field, and analyze combined data from tiltmeter and microseismic arrays. The hardware sections of the project included: (1) the building of new tiltmeter housings with feedthroughs for use in conjunction with a microseismic array, (2) the development of a means to use separate telemetry systems for the tilt and microseismic arrays, and (3) the selection and fabrication of an accelerometer sensor system to improve signal-to-noise ratios. The analysis sections of the project included a joint inversion for analysismore » and interpretation of combined tiltmeter and microseismic data and improved methods for extracting slippage planes and other reservoir information from the microseisms. In addition, testing was performed at various steps in the process to assess the data quality and problems/issues that arose during various parts of the project. A prototype array was successfully tested and a full array is now being fabricated for industrial use.« less

Fabrication and microstructures of functional gradient SiBCN–Nb composite by hot pressing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, Min, E-mail: lcxsunmin@163.com; Fu, Ruoyu; Chen, Jun

2016-04-15

A functional gradient material with five layers composed of SiBCN ceramic and niobium (Nb) was prepared successfully by hot pressing. The phase composition, morphology features and microstructures were investigated in each layer of the gradient material. The Nb-containing compounds involving NbC, Nb{sub 6}C{sub 5}, Nb{sub 4}C{sub 3}, Nb{sub 5}Si{sub 3} and NbN increase with the volume fraction of Nb increasing in the sub-layer. They are randomly scattered (≤ 25 vol.% Nb), then strip-like, and finally distribute continuously (≥ 75 vol.% Nb). The size of BN(C) and SiC grains in Nb-containing layers is larger than in 100% SiBCN layer due tomore » the loss of the capsule-like structures. No distinct interfaces form in the transition regions indicating the gradual changes in phase composition and microstructures. - Highlights: • A functional gradient SiBCN–Nb material was prepared successfully by hot pressing. • Phase composition, morphology features and microstructures were investigated. • Thermodynamic calculation was used to aid in the phase analysis. • No distinct interfaces form typical of the functional gradient material.« less

Cutaneous biocompatible rutin-loaded gelatin-based nanoparticles increase the SPF of the association of UVA and UVB filters.

PubMed

Oliveira, Camila Areias de; Peres, Daniela D'Almeida; Graziola, Fabiana; Chacra, Nádia Araci Bou; Araújo, Gabriel Lima Barros de; Flórido, Ana Catarina; Mota, Joana; Rosado, Catarina; Velasco, Maria Valéria Robles; Rodrigues, Luís Monteiro; Fernandes, Ana Sofia; Baby, André Rolim

2016-01-01

The encapsulation of natural ingredients, such as rutin, can offer improvements in sun protection effectiveness. This strategy can provide enhanced flavonoid content and produces an improved bioactive compound with new physical and functional characteristics. As an alternative to common synthetic-based sunscreens, rutin-entrapped gelatin nanoparticles (GNPs) were designed and associated with ethylhexyl dimethyl PABA (EHDP), ethylhexyl methoxycinnamate (EHMC) and methoxydibenzoylmethane (BMDBM) in sunscreen formulations. The purpose of this study was to develop rutin-loaded gelatin nanoparticles and characterize their physicochemical, thermal, functional and safety properties. Rutin-loaded gelatin nanoparticles increased antioxidant activity by 74% relative to free-rutin (FR) solution. Also, this new ingredient upgraded the Sun Protection Factor (SPF) by 48%, indicating its potential as a raw material for bioactive sunscreens. The safety profile indicated that GNPs and glutaraldehyde (GTA) decreased HaCaT cell viability in a concentration/time-dependent manner. However, both blank nanoparticles (B-NC) and rutin-loaded nanoparticles (R-NC) had good performance on skin compatibility tests. These results functionally characterized rutin-loaded nanoparticles as a safe SPF enhancer in sunscreens, especially in association with UV filters. Copyright © 2015 Elsevier B.V. All rights reserved.

Boron microlocalization in oral mucosal tissue: implications for boron neutron capture therapy

PubMed Central

Morris, G M; Smith, D R; Patel, H; Chandra, S; Morrison, G H; Hopewell, J W; Rezvani, M; Micca, P L; Coderre, J A

2000-01-01

Clinical studies of the treatment of glioma and cutaneous melanoma using boron neutron capture therapy (BNCT) are currently taking place in the USA, Europe and Japan. New BNCT clinical facilities are under construction in Finland, Sweden, England and California. The observation of transient acute effects in the oral mucosa of a number of glioma patients involved in the American clinical trials, suggests that radiation damage of the oral mucosa could be a potential complication in future BNCT clinical protocols, involving higher doses and larger irradiation field sizes. The present investigation is the first to use a high resolution surface analytical technique to relate the microdistribution of boron-10 (10B) in the oral mucosa to the biological effectiveness of the 10B(n,α)7Li neutron capture reaction in this tissue. The two boron delivery agents used clinically in Europe/Japan and the USA, borocaptate sodium (BSH) and p-boronophenylalanine (BPA), respectively, were evaluated using a rat ventral tongue model. 10B concentrations in various regions of the tongue mucosa were estimated using ion microscopy. In the epithelium, levels of 10B were appreciably lower after the administration of BSH than was the case after BPA. The epithelium:blood 10B partition ratios were 0.2:1 and 1:1 for BSH and BPA respectively. The 10B content of the lamina propria was higher than that measured in the epithelium for both BSH and BPA. The difference was most marked for BSH, where 10B levels were a factor of six higher in the lamina propria than in the epithelium. The concentration of 10B was also measured in blood vessel walls where relatively low levels of accumulation of BSH, as compared with BPA, was demonstrated in blood vessel endothelial cells and muscle. Vessel wall:blood 10B partition ratios were 0.3:1 and 0.9:1 for BSH and BPA respectively. Evaluation of tongue mucosal response (ulceration) to BNC irradiation indicated a considerably reduced radiation sensitivity using BSH as the boron delivery agent relative to BPA. The compound biological effectiveness (CBE) factor for BSH was estimated at 0.29 ± 0.02. This compares with a previously published CBE factor for BPA of 4.87 ± 0.16. It was concluded that variations in the microdistribution profile of 10B, using the two boron delivery agents, had a significant effect on the response of oral mucosa to BNC irradiation. From a clinical perspective, based on the findings of the present study, it is probable that potential radiation-induced oral mucositis will be restricted to BNCT protocols involving BPA. However, a thorough high resolution analysis of 10B microdistribution in human oral mucosal tissue, using a technique such as ion microscopy, is a prerequisite for the use of experimentally derived CBE factors in clinical BNCT. © 2000 Cancer Research Campaign PMID:10839288

Development of cable fed flash X-ray (FXR) system

NASA Astrophysics Data System (ADS)

Menon, Rakhee; Mitra, S.; Patel, A. S.; Kumar, R.; Singh, G.; Senthil, K.; Kumar, Ranjeet; Kolge, T. S.; Roy, Amitava; Acharya, S.; Biswas, D.; Sharma, Archana

2017-08-01

Flash X-ray sources driven by pulsed power find applications in industrial radiography, and a portable X-ray source is ideal where the radiography needs to be taken at the test site. A compact and portable flash X-ray (FXR) system based on a Marx generator has been developed with the high voltage fed to the FXR tube via a cable feed-through arrangement. Hard bremsstrahlung X-rays of few tens of nanosecond duration are generated by impinging intense electron beams on an anode target of high Z material. An industrial X-ray source is developed with source size as low as 1 mm. The system can be operated from 150 kV to 450 kV peak voltages and a dose of 10 mR has been measured at 1 m distance from the source window. The modeling of the FXR source has been carried out using particle-in-cell and Monte Carlo simulations for the electron beam dynamics and X-ray generation, respectively. The angular dose profile of X-ray has been measured and compared with the simulation.

Method and apparatus for performing in-situ vacuum-assisted metal to glass sealing

DOEpatents

Kramer, D.P.; Massey, R.T.

1985-07-18

A method and apparatus for assembling and fusing glass to metal in a glass-metal electrical component is disclosed. The component includes a metallic shell formed with upper and lower cylindrical recesses connected together by longitudinal passages, a pair of metal rings and plural metal pins assembled to define electrical feed-throughs. The component parts are assembled on a fixture having a sleeve-like projection and a central mounting projection establishing concentric nesting surfaces to which the metal rings are slip-fitted in concentric alignment with each other spaced from sidewalls of the lower recess. The pins are in electrical contact with the metal rings. A glass pre-form is seated within the upper recess. The assembled structure is heated to a temperature sufficient to melt the glass pre-form which flows under gravity through the passages into the lower recess to provide an insulative seal between the metal parts. The gravity flow of glass is assisted by applying vacuum to the lower recess, ensuring that all spaces between the metal parts are filled with sealing glass without formation of bubbles.

Method and apparatus for performing in-situ vacuum-assisted metal to glass sealing

DOEpatents

Kramer, Daniel P.; Massey, Richard T.

1986-01-01

A method and apparatus for assembling and fusing glass to metal in a glass-metal electrical component is disclosed. The component includes a metallic shell formed with upper and lower cylindrical recesses connected together by longitudinal passages, a pair of metal rings and plural metal pins assembled to define electrical feed-throughs. The component parts are assembled on a fixture having a sleeve-like projection and a central mounting projection establishing concentric nesting surfaces to which the metal rings are slip-fitted in concentric alignment with each other spaced from sidewalls of the lower recess. The pins are in electrical contact with the metal rings. A glass pre-form is seated within the upper recess. The assembled structure is heated to a temperature sufficient to melt the glass pre-form which flows under gravity through the passages into the lower recess to provide an insulative seal between the metal parts. The gravity flow of glass is assisted by applying vacuum to the lower recess, ensuring that all spaces between the metal parts are filled with sealing glass without formation of bubbles.

Beam position monitor engineering

NASA Astrophysics Data System (ADS)

Smith, Stephen R.

1997-01-01

The design of beam position monitors often involves challenging system design choices. Position transducers must be robust, accurate, and generate adequate position signal without unduly disturbing the beam. Electronics must be reliable and affordable, usually while meeting tough requirements on precision, accuracy, and dynamic range. These requirements may be difficult to achieve simultaneously, leading the designer into interesting opportunities for optimization or compromise. Some useful techniques and tools are shown. Both finite element analysis and analytic techniques will be used to investigate quasi-static aspects of electromagnetic fields such as the impedance of and the coupling of beam to striplines or buttons. Finite-element tools will be used to understand dynamic aspects of the electromagnetic fields of beams, such as wake fields and transmission-line and cavity effects in vacuum-to-air feedthroughs. Mathematical modeling of electrical signals through a processing chain will be demonstrated, in particular to illuminate areas where neither a pure time-domain nor a pure frequency-domain analysis is obviously advantageous. Emphasis will be on calculational techniques, in particular on using both time domain and frequency domain approaches to the applicable parts of interesting problems.

Mechanically coupled CMOS-MEMS free-free beam resonator arrays with enhanced power handling capability.

PubMed

Li, Ming-Huang; Chen, Wen-Chien; Li, Sheng-Shian

2012-03-01

Integrated CMOS-MEMS free-free beam resonator arrays operated in a standard two-port electrical configuration with low motional impedance and high power handling capability, centered at 10.5 MHz, have been demonstrated using the combination of pull-in gap reduction mechanism and mechanically coupled array design. The mechanical links (i.e., coupling elements) using short stubs connect each constituent resonator of an array to its adjacent ones at the high-velocity vibrating locations to accentuate the desired mode and reject all other spurious modes. A single second-mode free-free beam resonator with quality factor Q > 2200 and motional impedance R(m) < 150 kΩ has been used to achieve mechanically coupled resonator arrays in this work. In array design, a 9-resonator array has been experimentally characterized to have performance improvement of approximately 10× on motional impedance and power handling as compared with that of a single resonator. In addition, the two-port electrical configuration is much preferred over a one-port configuration because of its low-feedthrough and high design flexibility for future oscillator and filter implementation.

Prototype high speed optical delay line for stellar interferometry

NASA Astrophysics Data System (ADS)

Colavita, M. M.; Hines, B. E.; Shao, M.; Klose, G. J.; Gibson, B. V.

1991-12-01

The long baselines of the next-generation ground-based optical stellar interferometers require optical delay lines which can maintain nm-level path-length accuracy while moving at high speeds. NASA-JPL is currently designing delay lines to meet these requirements. The design is an enhanced version of the Mark III delay line, with the following key features: hardened, large diameter wheels, rather than recirculating ball bearings, to reduce mechanical noise; a friction-drive cart which bears the cable-dragging forces, and drives the optics cart through a force connection only; a balanced PZT assembly to enable high-bandwidth path-length control; and a precision aligned flexural suspension for the optics assembly to minimize bearing noise feedthrough. The delay line is fully programmable in position and velocity, and the system is controlled with four cascaded software feedback loops. Preliminary performance is a jitter in any 5 ms window of less than 10 nm rms for delay rates of up to 28 mm/s; total jitter is less than 10 nm rms for delay rates up to 20 mm/s.

High-Power Piezoelectric Acoustic-Electric Power Feedthru for Metal Walls

NASA Technical Reports Server (NTRS)

Bao, Xiaoqi; Biederman, Will; Sherrit, Stewart; Badescu, Mircea; Bar-Cohen, Yoseph; Jones, Christopher; Aldrich, Jack; Chang, Zensheu

2008-01-01

Piezoelectric acoustic-electric power feed-through devices transfer electric power wirelessly through a solid wall by using acoustic waves. This approach allows for the removal of holes through structures. The technology is applicable to power supply for electric equipment inside sealed containers, vacuum or pressure vessels, etc where the holes on the wall are prohibitive or result in significant performance degrade or complex designs. In the author's previous work, 100-W electric power was transferred through a metal wall by a small, simple-structure piezoelectric device. To meet requirements of higher power applications, the feasibility to transfer kilowatts level power was investigated. Pre-stressed longitudinal piezoelectric feedthru devices were analyzed by finite element model. An equivalent circuit model was developed to predict the power transfer characteristics to different electric loads. Based on the analysis results, a prototype device was designed, fabricated and a demonstration of the transmission of electric power up to 1-kW was successfully conducted. The methods to minimize the plate wave excitation on the wall were also analyzed. Both model analysis and experimental results are presented in detail in this presentation.

Key technologies for tritium storage bed development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yu, S.H.; Chang, M.H.; Kang, H.G.

2015-03-15

ITER Storage and Delivery System (SDS) is a complex system involving tens of storage beds. The most important SDS getter bed will be used for the absorption and desorption of hydrogen isotopes in accordance with the fusion fuel cycle scenario. In this paper the current status concerning research/development activities for the optimal approach to the final SDS design is introduced. A thermal analysis is performed and discussed on the aspect of heat losses considering whether the reflector and/or the feed-through is present or not. A thermal hydraulic simulation shows that the presence of 3 or 4 reflectors minimize the heatmore » loss. Another important point is to introduce the real-time gas analysis in the He{sup 3} collection system. In this study 2 independent strength methods based on gas chromatography and quadruple mass spectrometer for one and on a modified self-assaying quadruple mass spectrometer for the second are applied to separate the hydrogen isotopes in helium gas. Another issue is the possibility of using depleted uranium getter material for the storage of hydrogen isotopes, especially of tritium.« less

Killer whale caller localization using a hydrophone array in an oceanarium pool

NASA Astrophysics Data System (ADS)

Bowles, Ann E.; Greenlaw, Charles F.; McGehee, Duncan E.; van Holliday, D.

2004-05-01

A system to localize calling killer whales was designed around a ten-hydrophone array in a pool at SeaWorld San Diego. The array consisted of nine ITC 8212 and one ITC 6050H hydrophones mounted in recessed 30×30 cm2 niches. Eight of the hydrophones were connected to a Compaq Armada E500 laptop computer through a National Instruments DAQ 6024E PCMCIA A/D data acquisition card and a BNC-2120 signal conditioner. The system was calibrated with a 139-dB, 4.5-kHz pinger. Acoustic data were collected during four 48-72 h recording sessions, simultaneously with video recorded from a four-camera array. Calling whales were localized by one of two methods, (1) at the hydrophone reporting the highest sound exposure level and (2) using custom-designed 3-D localization software based on time-of-arrival (ORCA). Complex reverberations in the niches and pool made locations based on time of arrival difficult to collect. Based on preliminary analysis of data from four sessions (400+ calls/session), the hydrophone reporting the highest level reliably attributed callers 51%-100% of the time. This represents a substantial improvement over attribution rates of 5%-15% obtained with single hydrophone recordings. [Funding provided by Hubbs-SeaWorld Research Institute and the Hubbs Society.

X-ray Photon Counting Using 100 MHz Ready-Made Silicon P-Intrinsic-N X-ray Diode and Its Application to Energy-Dispersive Computed Tomography

NASA Astrophysics Data System (ADS)

Kodama, Hajime; Watanabe, Manabu; Sato, Eiichi; Oda, Yasuyuki; Hagiwara, Osahiko; Matsukiyo, Hiroshi; Osawa, Akihiro; Enomoto, Toshiyuki; Kusachi, Shinya; Sato, Shigehiro; Ogawa, Akira

2013-07-01

X-ray photons are directly detected using a 100 MHz ready-made silicon P-intrinsic-N X-ray diode (Si-PIN-XD). The Si-PIN-XD is shielded using an aluminum case with a 25-µm-thick aluminum window and a BNC connector. The photocurrent from the Si-PIN-XD is amplified by charge sensitive and shaping amplifiers, and the event pulses are sent to a multichannel analyzer (MCA) to measure X-ray spectra. At a tube voltage of 90 kV, we observe K-series characteristic X-rays of tungsten. Photon-counting computed tomography (PC-CT) is accomplished by repeated linear scans and rotations of an object, and projection curves of the object are obtained by linear scanning at a tube current of 2.0 mA. The exposure time for obtaining a tomogram is 10 min with scan steps of 0.5 mm and rotation steps of 1.0°. At a tube voltage of 90 kV, the maximum count rate is 150 kcps. We carry out PC-CT using gadolinium media and confirm the energy-dispersive effect with changes in the lower level voltage of the event pulse using a comparator.

GWAS reveals loci associated with velopharyngeal dysfunction.

PubMed

Chernus, Jonathan; Roosenboom, Jasmien; Ford, Matthew; Lee, Myoung Keun; Emanuele, Beth; Anderton, Joel; Hecht, Jacqueline T; Padilla, Carmencita; Deleyiannis, Frederic W B; Buxo, Carmen J; Feingold, Eleanor; Leslie, Elizabeth J; Shaffer, John R; Weinberg, Seth M; Marazita, Mary L

2018-05-31

Velopharyngeal dysfunction (VPD) occurs when the muscular soft palate (velum) and lateral pharyngeal walls are physically unable to separate the oral and nasal cavities during speech production leading to hypernasality and abnormal speech reduction. Because VPD is often associated with overt or submucous cleft palate, it could be present as a subclinical phenotype in families with a history of orofacial clefting. A key assumption to this model is that the overt and subclinical manifestations of the orofacial cleft phenotype exist on a continuum and therefore share common etiological factors. We performed a genome-wide association study in 976 unaffected relatives of isolated CP probands, 54 of whom had VPD. Five loci were significantly (p < 5 × 10 -8 ) associated with VPD: 3q29, 9p21.1, 12q21.31, 16p12.3 and 16p13.3. An additional 15 loci showing suggestive evidence of association with VPD were observed. Several genes known to be involved in orofacial clefting and craniofacial development are located in these regions, such as TFRC, PCYT1A, BNC2 and FREM1. Although further research is necessary, this could be an indication for a potential shared genetic architecture between VPD and cleft palate, and supporting the hypothesis that VPD is a subclinical phenotype of orofacial clefting.

Genomic anatomy of the Tyrp1 (brown) deletion complex

PubMed Central

Smyth, Ian M.; Wilming, Laurens; Lee, Angela W.; Taylor, Martin S.; Gautier, Phillipe; Barlow, Karen; Wallis, Justine; Martin, Sancha; Glithero, Rebecca; Phillimore, Ben; Pelan, Sarah; Andrew, Rob; Holt, Karen; Taylor, Ruth; McLaren, Stuart; Burton, John; Bailey, Jonathon; Sims, Sarah; Squares, Jan; Plumb, Bob; Joy, Ann; Gibson, Richard; Gilbert, James; Hart, Elizabeth; Laird, Gavin; Loveland, Jane; Mudge, Jonathan; Steward, Charlie; Swarbreck, David; Harrow, Jennifer; North, Philip; Leaves, Nicholas; Greystrong, John; Coppola, Maria; Manjunath, Shilpa; Campbell, Mark; Smith, Mark; Strachan, Gregory; Tofts, Calli; Boal, Esther; Cobley, Victoria; Hunter, Giselle; Kimberley, Christopher; Thomas, Daniel; Cave-Berry, Lee; Weston, Paul; Botcherby, Marc R. M.; White, Sharon; Edgar, Ruth; Cross, Sally H.; Irvani, Marjan; Hummerich, Holger; Simpson, Eleanor H.; Johnson, Dabney; Hunsicker, Patricia R.; Little, Peter F. R.; Hubbard, Tim; Campbell, R. Duncan; Rogers, Jane; Jackson, Ian J.

2006-01-01

Chromosome deletions in the mouse have proven invaluable in the dissection of gene function. The brown deletion complex comprises >28 independent genome rearrangements, which have been used to identify several functional loci on chromosome 4 required for normal embryonic and postnatal development. We have constructed a 172-bacterial artificial chromosome contig that spans this 22-megabase (Mb) interval and have produced a contiguous, finished, and manually annotated sequence from these clones. The deletion complex is strikingly gene-poor, containing only 52 protein-coding genes (of which only 39 are supported by human homologues) and has several further notable genomic features, including several segments of >1 Mb, apparently devoid of a coding sequence. We have used sequence polymorphisms to finely map the deletion breakpoints and identify strong candidate genes for the known phenotypes that map to this region, including three lethal loci (l4Rn1, l4Rn2, and l4Rn3) and the fitness mutant brown-associated fitness (baf). We have also characterized misexpression of the basonuclin homologue, Bnc2, associated with the inversion-mediated coat color mutant white-based brown (Bw). This study provides a molecular insight into the basis of several characterized mouse mutants, which will allow further dissection of this region by targeted or chemical mutagenesis. PMID:16505357

B and N isolate-doped graphitic carbon nanosheets from nitrogen-containing ion-exchanged resins for enhanced oxygen reduction

NASA Astrophysics Data System (ADS)

Wang, Lei; Yu, Peng; Zhao, Lu; Tian, Chungui; Zhao, Dongdong; Zhou, Wei; Yin, Jie; Wang, Ruihong; Fu, Honggang

2014-06-01

B,N-codoped carbon nanostructures (BNCS) can serve as alternative low-cost metal-free electrocatalysts for oxygen reduction reactions (ORR). However, the compensation effect between the p- (B atoms) and n-type (N atoms) dopants would make the covalent boron-nitride (BN) easily formed during the synthesis of BNCS, leading to a unsatisfactory ORR activity. Therefore, it has been challenging to develop facile and rapid synthetic strategies for highly active BNCS without forming the direct covalent BN. Here, a facile method is developed to prepare B and N isolate-doped graphitic nanosheets (BNGS) by using iron species for saving N element and simultaneous doping the B element from nitrogen-containing ion-exchanged resins (NR). The resulting BNGS exhibits much more onset potential (Eonset) compared with the B-doped graphitic carbon nanosheets (BGS), N-doped graphitic carbon nanosheets (NGS), as well as B,N-codoped disorder carbon (BNC). Moreover, the BNGS shows well methanol tolerance propery and excellent stability (a minimal loss of activity after 5,000 potential cycles) compared to that of commercial Pt/C catalyst. The goog performance for BNGS towards ORR is attributed to the synergistic effect between B and N, and the well electrons transport property of graphitic carbon in BNGS.

Synthesis of boron, nitrogen co-doped porous carbon from asphaltene for high-performance supercapacitors

NASA Astrophysics Data System (ADS)

Zhou, Ying; Wang, Dao-Long; Wang, Chun-Lei; Jin, Xin-Xin; Qiu, Jie-Shan

2014-08-01

Oxidized asphaltene (OA), a thermosetting material with plenty of functional groups, is synthesized from asphaltene (A) using HNO3/H2SO4 as the oxidizing agent. Boron, nitrogen co-doped porous carbon (BNC—OA) is prepared by carbonization of the mixture of boric acid and OA at 1173 K in an argon atmosphere. X-ray photoelectron spectroscopy (XPS) characterization reveals that the BNC—OA has a nitrogen content of 3.26 at.% and a boron content of 1.31 at.%, while its oxidation-free counterpart (BNC—SA) has a nitrogen content of 1.61 at.% and a boron content of 3.02 at.%. The specific surface area and total pore volume of BNC—OA are 1103 m2·g-1 and 0.921 cm3·g-1, respectively. At a current density of 0.1 A·g-1, the specific capacitance of BNC-OA is 335 F·g-1 and the capacitance retention can still reach 83% at 1 A·g-1. The analysis shows that the superior electrochemical performance of the BNC—OA is attributed to the pseudocapacitance behavior of surface heteroatom functional groups and an abundant pore-structure. Boron, nitrogen co-doped porous carbon is a promising electrode material for supercapacitors.

Biodegradation of PuEDTA and Impacts on Pu Mobility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bolton, H., Jr.; Rai, D.; Xun, L.

The contamination of many DOE sites by Pu presents a long-term problem because of its long half-life (240,000 yrs) and the low drinking water standard (<10{sup -12} M). EDTA was co-disposed with radionuclides (e.g., Pu, {sup 60}Co), formed strong complexes, and enhanced radionuclide transport at several DOE sites. Biodegradation of EDTA should decrease Pu mobility. One objective of this project was to determine the biodegradation of EDTA in the presence of PuEDTA complexes. The aqueous system investigated at pH 7 (10{sup -4} M EDTA and 10{sup -6} M Pu) contained predominantly Pu(OH){sub 2}EDTA{sup 2-}. The EDTA was degraded at amore » faster rate in the presence of Pu. As the total concentration of both EDTA and PuEDTA decreased (i.e., 10{sup -5} M EDTA and 10{sup -7} M PuEDTA), the presence of Pu decreased the biodegradation rate of the EDTA. It is currently unclear why the concentration of Pu directly affects the increase/decrease in rate of EDTA biodegradation. The soluble Pu concentration decreased, in agreement with thermodynamic predictions, as the EDTA was biodegraded, indicating that biodegradation of EDTA will decrease Pu mobility when the Pu is initially present as Pu(IV)EDTA. A second objective was to investigate how the presence of competing metals, commonly encountered in geologic media, will influence the speciation and biodegradation of Pu(IV)-EDTA. Studies on the solubilities of Fe(OH){sub 3}(s) and of Fe(OH){sub 3}(s) plus PuO{sub 2}(am) in the presence of EDTA and as a function of pH showed that Fe(III) out competes the Pu(IV) for the EDTA complex, thereby showing that Pu(IV) will not form stable complexes with EDTA for enhanced transport of Pu in Fe(III) dominated subsurface systems. A third objective is to investigate the genes and enzymes involved in EDTA biodegradation. BNC1 can use EDTA and another synthetic chelating agent nitrilotriacetate (NTA) as sole carbon and nitrogen sources. The same catabolic enzymes are responsible for both EDTA and NTA degradation except that additional enzymes are required for EDTA degradation. When the catabolic genes were cloned and sequenced, the gene cluster also contained genes encoding a hypothetical ABC-type transporter. RT-PCR analysis showed that the transporter genes and EDTA monooxygenase gene (emoA) are co-transcribed. EppA is one of the transporter genes, and it codes for a periplasmic binding protein responsible for binding to the substrate before transport across the membrane can occur. EppA was cloned, expressed, and purified in Escherichia coli and found to bind, MgEDTA, CaEDTA, Fe(III)EDTA, MgNTA, CaNTA, and Fe(III)NTA. Our data also suggest that BNC1 uses the same ABC-type transporter for both EDTA and NTA uptake. Results from these studies can provide mechanistic understanding and approaches to assist in the bioremediate PuEDTA and other radionuclide-EDTA complexes at DOE sites.« less

Potent In Vivo NK Cell-Mediated Elimination of HIV-1-Infected Cells Mobilized by a gp120-Bispecific and Hexavalent Broadly Neutralizing Fusion Protein

PubMed Central

Bardhi, Ariola; Wu, Yanling; Chen, Weizao; Li, Wei; Zhu, Zhongyu; Zheng, Jian Hua; Wong, Hing; Jeng, Emily; Jones, Jennifer; Ochsenbauer, Christina; Kappes, John C.; Dimitrov, Dimiter S.; Ying, Tianlei

2017-01-01

ABSTRACT Antibodies bound to human immunodeficiency virus type 1 (HIV-1) envelope protein expressed by infected cells mobilize antibody-dependent cellular cytotoxicity (ADCC) to eliminate the HIV-1-infected cells and thereby suppress HIV-1 infection and delay disease progression. Studies treating HIV-1-infected individuals with latency reactivation agents to reduce their latent HIV-1 reservoirs indicated that their HIV-1-specific immune responses were insufficient to effectively eliminate the reactivated latent HIV-1-infected T cells. Mobilization of ADCC may facilitate elimination of reactivated latent HIV-1-infected cells to deplete the HIV-1 reservoir and contribute to a functional HIV-1 cure. The most effective antibodies for controlling and eradicating HIV-1 infection would likely have the dual capacities of potently neutralizing a broad range of HIV-1 isolates and effectively mobilizing HIV-1-specific ADCC to eliminate HIV-1-infected cells. For this purpose, we constructed LSEVh-LS-F, a broadly neutralizing, defucosylated hexavalent fusion protein specific for both the CD4 and coreceptor gp120-binding sites. LSEVh-LS-F potently inhibited in vivo HIV-1 and simian-human immunodeficiency virus (SHIV) infection in humanized mouse and macaque models, respectively, including in vivo neutralization of HIV-1 strains resistant to the broadly neutralizing antibodies VRC01 and 3BNC117. We developed a novel humanized mouse model to evaluate in vivo human NK cell-mediated elimination of HIV-1-infected cells by ADCC and utilized it to demonstrate that LSEVh-LS-F rapidly mobilized NK cells to eliminate >80% of HIV-1-infected cells in vivo 1 day after its administration. The capacity of LSEVh-LS-F to eliminate HIV-1-infected cells via ADCC combined with its broad neutralization activity supports its potential use as an immunotherapeutic agent to eliminate reactivated latent cells and deplete the HIV-1 reservoir. IMPORTANCE Mobilization of antibody-dependent cellular cytotoxicity (ADCC) to eliminate reactivated latent HIV-1-infected cells is a strategy which may contribute to depleting the HIV-1 reservoir and achieving a functional HIV-1 cure. To more effectively mobilize ADCC, we designed and constructed LSEVh-LS-F, a broadly neutralizing, defucosylated hexavalent fusion protein specific for both the CD4 and coreceptor gp120-binding sites. LSEVh-LS-F potently inhibited in vivo HIV-1 and SHIV infection in humanized mouse and macaque models, respectively, including in vivo neutralization of an HIV-1 strain resistant to the broadly neutralizing antibodies VRC01 and 3BNC117. Using a novel humanized mouse model, we demonstrated that LSEVh-LS-F rapidly mobilized NK cells to eliminate >80% of HIV-1-infected cells in vivo 1 day after its administration. The capacity of LSEVh-LS-F to eliminate HIV-1-infected cells via ADCC combined with its broad neutralization activity supports its potential use as an immunotherapeutic agent to eliminate reactivated latent cells and deplete the HIV-1 reservoir. PMID:28794022

Potent In Vivo NK Cell-Mediated Elimination of HIV-1-Infected Cells Mobilized by a gp120-Bispecific and Hexavalent Broadly Neutralizing Fusion Protein.

PubMed

Bardhi, Ariola; Wu, Yanling; Chen, Weizao; Li, Wei; Zhu, Zhongyu; Zheng, Jian Hua; Wong, Hing; Jeng, Emily; Jones, Jennifer; Ochsenbauer, Christina; Kappes, John C; Dimitrov, Dimiter S; Ying, Tianlei; Goldstein, Harris

2017-10-15

Antibodies bound to human immunodeficiency virus type 1 (HIV-1) envelope protein expressed by infected cells mobilize antibody-dependent cellular cytotoxicity (ADCC) to eliminate the HIV-1-infected cells and thereby suppress HIV-1 infection and delay disease progression. Studies treating HIV-1-infected individuals with latency reactivation agents to reduce their latent HIV-1 reservoirs indicated that their HIV-1-specific immune responses were insufficient to effectively eliminate the reactivated latent HIV-1-infected T cells. Mobilization of ADCC may facilitate elimination of reactivated latent HIV-1-infected cells to deplete the HIV-1 reservoir and contribute to a functional HIV-1 cure. The most effective antibodies for controlling and eradicating HIV-1 infection would likely have the dual capacities of potently neutralizing a broad range of HIV-1 isolates and effectively mobilizing HIV-1-specific ADCC to eliminate HIV-1-infected cells. For this purpose, we constructed LSEVh-LS-F, a broadly neutralizing, defucosylated hexavalent fusion protein specific for both the CD4 and coreceptor gp120-binding sites. LSEVh-LS-F potently inhibited in vivo HIV-1 and simian-human immunodeficiency virus (SHIV) infection in humanized mouse and macaque models, respectively, including in vivo neutralization of HIV-1 strains resistant to the broadly neutralizing antibodies VRC01 and 3BNC117. We developed a novel humanized mouse model to evaluate in vivo human NK cell-mediated elimination of HIV-1-infected cells by ADCC and utilized it to demonstrate that LSEVh-LS-F rapidly mobilized NK cells to eliminate >80% of HIV-1-infected cells in vivo 1 day after its administration. The capacity of LSEVh-LS-F to eliminate HIV-1-infected cells via ADCC combined with its broad neutralization activity supports its potential use as an immunotherapeutic agent to eliminate reactivated latent cells and deplete the HIV-1 reservoir. IMPORTANCE Mobilization of antibody-dependent cellular cytotoxicity (ADCC) to eliminate reactivated latent HIV-1-infected cells is a strategy which may contribute to depleting the HIV-1 reservoir and achieving a functional HIV-1 cure. To more effectively mobilize ADCC, we designed and constructed LSEVh-LS-F, a broadly neutralizing, defucosylated hexavalent fusion protein specific for both the CD4 and coreceptor gp120-binding sites. LSEVh-LS-F potently inhibited in vivo HIV-1 and SHIV infection in humanized mouse and macaque models, respectively, including in vivo neutralization of an HIV-1 strain resistant to the broadly neutralizing antibodies VRC01 and 3BNC117. Using a novel humanized mouse model, we demonstrated that LSEVh-LS-F rapidly mobilized NK cells to eliminate >80% of HIV-1-infected cells in vivo 1 day after its administration. The capacity of LSEVh-LS-F to eliminate HIV-1-infected cells via ADCC combined with its broad neutralization activity supports its potential use as an immunotherapeutic agent to eliminate reactivated latent cells and deplete the HIV-1 reservoir. Copyright © 2017 American Society for Microbiology.

In vivo biocompatibility of boron doped and nitrogen included conductive-diamond for use in medical implants.

PubMed

Garrett, David J; Saunders, Alexia L; McGowan, Ceara; Specks, Joscha; Ganesan, Kumaravelu; Meffin, Hamish; Williams, Richard A; Nayagam, David A X

2016-01-01

Recently, there has been interest in investigating diamond as a material for use in biomedical implants. Diamond can be rendered electrically conducting by doping with boron or nitrogen. This has led to inclusion of boron doped and nitrogen included diamond elements as electrodes and/or feedthroughs for medical implants. As these conductive device elements are not encapsulated, there is a need to establish their clinical safety for use in implants. This article compares the biocompatibility of electrically conducting boron doped diamond (BDD) and nitrogen included diamond films and electrically insulating poly crystalline diamond films against a silicone negative control and a BDD sample treated with stannous octoate as a positive control. Samples were surgically implanted into the back muscle of a guinea pig for a period of 4-15 weeks, excised and the implant site sectioned and submitted for histological analysis. All forms of diamond exhibited a similar or lower thickness of fibrotic tissue encapsulating compared to the silicone negative control samples. All forms of diamond exhibited similar or lower levels of acute, chronic inflammatory, and foreign body responses compared to the silicone negative control indicating that the materials are well tolerated in vivo. © 2015 Wiley Periodicals, Inc.

Using Drained Spacecraft Propellant Tanks for Habitation

NASA Technical Reports Server (NTRS)

Thomas, Andrew S. W.

2009-01-01

A document proposes that future spacecraft for planetary and space exploration be designed to enable reuse of drained propellant tanks for occupancy by humans. This proposal would enable utilization of volume and mass that would otherwise be unavailable and, in some cases, discarded. Such utilization could enable reductions in cost, initial launch mass, and number of launches needed to build up a habitable outpost in orbit about, or on the surface of, a planet or moon. According to the proposal, the large propellant tanks of a spacecraft would be configured to enable crews to gain access to their interiors. The spacecraft would incorporate hatchways, between a tank and the crew volume, that would remain sealed while the tank contained propellant and could be opened after the tank was purged by venting to outer space and then refilled with air. The interior of the tank would be pre-fitted with some habitation fixtures that were compatible with the propellant environment. Electrical feed-throughs, used originally for gauging propellants, could be reused to supply electric power to equipment installed in the newly occupied space. After a small amount of work, the tank would be ready for long-term use as a habitation module.

Progress in Acoustic Transmission of Power through Walls

NASA Technical Reports Server (NTRS)

Sherrit,Stewart; Coty, Benjamin; Bao, Xiaoqi; Bar-Cohen, Yoseph; Badescu, Mircea; Chang, Zensheu

2008-01-01

A document presents updated information on implementation of the wireless acoustic-electric feed-through (WAEF) concept, which was reported in Using Piezoelectric Devices To Transmit Power Through Walls (NPO-41157), NASA Tech Briefs, Vol. 32, No. 6 (June 2008), page 70. To recapitulate: In a basic WAEF setup, a transmitting piezoelectric transducer on one side of a wall is driven at resonance to excite ultrasonic vibrations in the wall. A receiving piezoelectric transducer on the opposite side of the wall converts the vibrations back to an ultrasonic AC electric signal, which is then detected and otherwise processed in a manner that depends on the modulation (if any) applied to the signal and whether the signal is used to transmit power, data, or both. The present document expands upon the previous information concerning underlying physical principles, advantages, and potential applications of WAEF. It discusses the design and construction of breadboard prototype piezoelectric transducers for WAEF. It goes on to present results of computational simulations of performance and results of laboratory tests of the prototypes. In one notable test, a 100-W light bulb was lit by WAEF to demonstrate the feasibility of powering a realistic load.

Fractional order implementation of Integral Resonant Control - A nanopositioning application.

PubMed

San-Millan, Andres; Feliu-Batlle, Vicente; Aphale, Sumeet S

2017-10-04

By exploiting the co-located sensor-actuator arrangement in typical flexure-based piezoelectric stack actuated nanopositioners, the polezero interlacing exhibited by their axial frequency response can be transformed to a zero-pole interlacing by adding a constant feed-through term. The Integral Resonant Control (IRC) utilizes this unique property to add substantial damping to the dominant resonant mode by the use of a simple integrator implemented in closed loop. IRC used in conjunction with an integral tracking scheme, effectively reduces positioning errors introduced by modelling inaccuracies or parameter uncertainties. Over the past few years, successful application of the IRC control technique to nanopositioning systems has demonstrated performance robustness, easy tunability and versatility. The main drawback has been the relatively small positioning bandwidth achievable. This paper proposes a fractional order implementation of the classical integral tracking scheme employed in tandem with the IRC scheme to deliver damping and tracking. The fractional order integrator introduces an additional design parameter which allows desired pole-placement, resulting in superior closed loop bandwidth. Simulations and experimental results are presented to validate the theory. A 250% improvement in the achievable positioning bandwidth is observed with proposed fractional order scheme. Copyright © 2017. Published by Elsevier Ltd.

Plasma electron analysis: Voyager plasma science experiment

NASA Technical Reports Server (NTRS)

Sittler, E. C., Jr.

1983-01-01

The Plasma Science Experiment (PLS) on the Voyager spacecraft provide data on the plasma ions and electrons in the interplanetary medium and the magnetospheres of the giant planets Jupiter and Saturn. A description of the analysis used to obtain electron parameters (density, temperature, etc.) from the plasma science experiment PLS electron measurements which cover the energy range from 10 eV to 5950 eV is presented. The electron sensor (D cup) and its transmission characteristics are described. A derivation of the fundamental analytical expression of the reduced distribution function F(e) is given. The electron distribution function F(e), used in the moment integrations, can be derived from F(e). Positive ions produce a correction current (ion feedthrough) to the measured electron current, which can be important to the measurements of the suprathermal electron component. In the case of Saturn, this correction current, which can either add to or subtract from the measured electron current, is less than 20% of the measured signal at all times. Comments about the corrections introduced by spacecraft charging to the Saturn encounter data, which can be important in regions of high density and shadow when the spacecraft can become negatively charged are introduced.

Application of Au-Sn eutectic bonding in hermetic radio-frequency microelectromechanical system wafer level packaging

NASA Astrophysics Data System (ADS)

Wang, Qian; Choa, Sung-Hoon; Kim, Woonbae; Hwang, Junsik; Ham, Sukjin; Moon, Changyoul

2006-03-01

Development of packaging is one of the critical issues toward realizing commercialization of radio-frequency-microelectromechanical system (RF-MEMS) devices. The RF-MEMS package should be designed to have small size, hermetic protection, good RF performance, and high reliability. In addition, packaging should be conducted at sufficiently low temperature. In this paper, a low-temperature hermetic wafer level packaging scheme for the RF-MEMS devices is presented. For hermetic sealing, Au-Sn eutectic bonding technology at temperatures below 300°C is used. Au-Sn multilayer metallization with a square loop of 70 µm in width is performed. The electrical feed-through is achieved by the vertical through-hole via filling with electroplated Cu. The size of the MEMS package is 1 mm × 1 mm × 700 µm. The shear strength and hermeticity of the package satisfies the requirements of MIL-STD-883F. Any organic gases or contamination are not observed inside the package. The total insertion loss for the packaging is 0.075 dB at 2 GHz. Furthermore, the robustness of the package is demonstrated by observing no performance degradation and physical damage of the package after several reliability tests.

Lessons Learned from the Space Shuttle Engine Cutoff System (ECO) Anomalies

NASA Technical Reports Server (NTRS)

Martinez, Hugo E.; Welzyn, Ken

2011-01-01

The Space Shuttle Orbiter's main engine cutoff (ECO) system first failed ground checkout in April, 2005 during a first tanking test prior to Return-to-Flight. Despite significant troubleshooting and investigative efforts that followed, the root cause could not be found and intermittent anomalies continued to plague the Program. By implementing hardware upgrades, enhancing monitoring capability, and relaxing the launch rules, the Shuttle fleet was allowed to continue flying in spite of these unexplained failures. Root cause was finally determined following the launch attempts of STS-122 in December, 2007 when the anomalies repeated, which allowed drag-on instrumentation to pinpoint the fault (the ET feedthrough connector). The suspect hardware was removed and provided additional evidence towards root cause determination. Corrective action was implemented and the system has performed successfully since then. This white paper presents the lessons learned from the entire experience, beginning with the anomalies since Return-to-Flight through discovery and correction of the problem. To put these lessons in better perspective for the reader, an overview of the ECO system is presented first. Next, a chronological account of the failures and associated investigation activities is discussed. Root cause and corrective action are summarized, followed by the lessons learned.

A Hermetic Wireless Subretinal Neurostimulator for Vision Prostheses

PubMed Central

Shire, Douglas B.; Chen, Jinghua; Doyle, Patrick; Gingerich, Marcus D.; Cogan, Stuart F.; Drohan, William A.; Behan, Sonny; Theogarajan, Luke; Wyatt, John L.; Rizzo, Joseph F.

2016-01-01

A miniaturized, hermetically encased, wirelessly operated retinal prosthesis has been developed for preclinical studies in the Yucatan minipig, and includes several design improvements over our previously reported device. The prosthesis attaches conformally to the outside of the eye and electrically drives a microfabricated thin-film polyimide array of sputtered iridium oxide film electrodes. This array is implanted into the subretinal space using a customized ab externo surgical technique. The implanted device includes a hermetic titanium case containing a 15-channel stimulator chip and discrete circuit components. Feedthroughs in the case connect the stimulator chip to secondary power and data receiving coils on the eye and to the electrode array under the retina. Long-term in vitro pulse testing of the electrodes projected a lifetime consistent with typical devices in industry. The final assembly was tested in vitro to verify wireless operation of the system in physiological saline using a custom RF transmitter and primary coils. Stimulation pulse strength, duration, and frequency were programmed wirelessly from a Peripheral Component Interconnect eXtensions for Instrumentation (PXI) computer. Operation of the retinal implant has been verified in two pigs for up to five and a half months by detecting stimulus artifacts generated by the implanted device. PMID:21859595

Validation Assessment of a Glass-to-Metal Seal Finite-Element Model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jamison, Ryan Dale; Buchheit, Thomas E.; Emery, John M

Sealing glasses are ubiquitous in high pressure and temperature engineering applications, such as hermetic feed-through electrical connectors. A common connector technology are glass-to-metal seals where a metal shell compresses a sealing glass to create a hermetic seal. Though finite-element analysis has been used to understand and design glass-to-metal seals for many years, there has been little validation of these models. An indentation technique was employed to measure the residual stress on the surface of a simple glass-to-metal seal. Recently developed rate- dependent material models of both Schott 8061 and 304L VAR stainless steel have been applied to a finite-element modelmore » of the simple glass-to-metal seal. Model predictions of residual stress based on the evolution of material models are shown. These model predictions are compared to measured data. Validity of the finite- element predictions is discussed. It will be shown that the finite-element model of the glass-to-metal seal accurately predicts the mean residual stress in the glass near the glass-to-metal interface and is valid for this quantity of interest.« less

Method of forming a package for MEMS-based fuel cell

DOEpatents

Morse, Jeffrey D; Jankowski, Alan F

2013-05-21

A MEMS-based fuel cell package and method thereof is disclosed. The fuel cell package comprises seven layers: (1) a sub-package fuel reservoir interface layer, (2) an anode manifold support layer, (3) a fuel/anode manifold and resistive heater layer, (4) a Thick Film Microporous Flow Host Structure layer containing a fuel cell, (5) an air manifold layer, (6) a cathode manifold support structure layer, and (7) a cap. Fuel cell packages with more than one fuel cell are formed by positioning stacks of these layers in series and/or parallel. The fuel cell package materials such as a molded plastic or a ceramic green tape material can be patterned, aligned and stacked to form three dimensional microfluidic channels that provide electrical feedthroughs from various layers which are bonded together and mechanically support a MEMS-based miniature fuel cell. The package incorporates resistive heating elements to control the temperature of the fuel cell stack. The package is fired to form a bond between the layers and one or more microporous flow host structures containing fuel cells are inserted within the Thick Film Microporous Flow Host Structure layer of the package.

Method of forming a package for mems-based fuel cell

DOEpatents

Morse, Jeffrey D.; Jankowski, Alan F.

2004-11-23

A MEMS-based fuel cell package and method thereof is disclosed. The fuel cell package comprises seven layers: (1) a sub-package fuel reservoir interface layer, (2) an anode manifold support layer, (3) a fuel/anode manifold and resistive heater layer, (4) a Thick Film Microporous Flow Host Structure layer containing a fuel cell, (5) an air manifold layer, (6) a cathode manifold support structure layer, and (7) a cap. Fuel cell packages with more than one fuel cell are formed by positioning stacks of these layers in series and/or parallel. The fuel cell package materials such as a molded plastic or a ceramic green tape material can be patterned, aligned and stacked to form three dimensional microfluidic channels that provide electrical feedthroughs from various layers which are bonded together and mechanically support a MEMOS-based miniature fuel cell. The package incorporates resistive heating elements to control the temperature of the fuel cell stack. The package is fired to form a bond between the layers and one or more microporous flow host structures containing fuel cells are inserted within the Thick Film Microporous Flow Host Structure layer of the package.

NASA Tech Briefs, June 2009

NASA Technical Reports Server (NTRS)

2009-01-01

Topics covered include: Device for Measuring Low Flow Speed in a Duct, Measuring Thermal Conductivity of a Small Insulation Sample, Alignment Jig for the Precise Measurement of THz Radiation, Autoignition Chamber for Remote Testing of Pyrotechnic Devices, Microwave Power Combiners for Signals of Arbitrary Amplitude, Synthetic Foveal Imaging Technology, Airborne Antenna System for Minimum-Cycle-Slip GPS Reception, Improved Starting Materials for Back-Illuminated Imagers, Multi-Modulator for Bandwidth-Efficient Communication, Some Improvements in Utilization of Flash Memory Devices, GPS/MEMS IMU/Microprocessor Board for Navigation, T/R Multi-Chip MMIC Modules for 150 GHz, Pneumatic Haptic Interfaces, Device Acquires and Retains Rock or Ice Samples, Cryogenic Feedthrough Test Rig, Improved Assembly for Gas Shielding During Welding or Brazing, Two-Step Plasma Process for Cleaning Indium Bonding Bumps, Tool for Crimping Flexible Circuit Leads, Yb14MnSb11 as a High-Efficiency Thermoelectric Material, Polyimide-Foam/Aerogel Composites for Thermal Insulation, Converting CSV Files to RKSML Files, Service Management Database for DSN Equipment, Chemochromic Hydrogen Leak Detectors, Compatibility of Segments of Thermoelectric Generators, Complementary Barrier Infrared Detector, JPL Greenland Moulin Exploration Probe, Ultra-Lightweight Self-Deployable Nanocomposite Structure for Habitat Applications, and Room-Temperature Ionic Liquids for Electrochemical Capacitors.

Frequency response improvement of a two-port surface acoustic wave device based on epitaxial AlN thin film

NASA Astrophysics Data System (ADS)

Gao, Junning; Hao, Zhibiao; Luo, Yi; Li, Guoqiang

2018-01-01

This paper presents an exploration on improving the frequency response of the symmetrical two-port AlN surface acoustic wave (SAW) device, using epitaxial AlN thin film on (0001) sapphire as the piezoelectric substrate. The devices were fabricated by lift-off processes with Ti/Al composite electrodes as interleaved digital transducers (IDT). The impact of DL and the number of the IDT finger pairs on the frequency response was carefully investigated. The overall properties of the device are found to be greatly improved with DL elongation, indicated by the reduced pass band ripple and increased stop band rejection ratio. The rejection increases by 8.3 dB when DL elongates from 15.5λ to 55.5λ and 4.4 dB further accompanying another 50λ elongation. This is because larger DL repels the stray acoustic energy out of the propagation path and provides a cleaner traveling channel for functional SAW, and at the same time restrains electromagnetic feedthrough. It is also found that proper addition of the IDT finger pairs is beneficial for the device response, indicated by the ripple reduction and the insertion loss drop.

Development and Testing of a ``Backlash-Free'' Gas-Tight High Precision Sample Handling Mechanism for Combined Science on the ExoMars 2018 Rover

NASA Astrophysics Data System (ADS)

Paul, R.; Redlich, D.; Richter, L.; Zuknik, K.-H.; Muhlbauer, Q.; Thiel, M.; Fowler, L.; Tattusch, T.; Weisz, H.; Musso, F.; Durrant, S.

2015-09-01

This paper presents the development and testing by the OHB System AG of the Powdered Sample Handling Mechanism (PSHS) that is part of the rover of the European Space Agency 2018 ExoMars Mission, a cooperative mission with Roscosmos including a scientific instrument contribution from NASA. The task of this mechanism is to flatten and position powdered Martian soil samples allowing subsequent investigation of selected grains by different optical instruments thus providing combined science in an ultra-clean environment.The exceptional sensitivity of these instruments causes extremely challenging requirements with respect to positioning performance as well as cleanliness and contamination control. The impact of these design drivers is highlighted focusing on specific mechanism features such as the pre-torque device to minimize the backlash and the dynamic feed-through, allowing a gas-tight encapsulation of an ultra-clean zone free of drive-train components.Subsequently the results of the test campaign of an elegant breadboard under Mars-like conditions, as well as first QM test results are described. Furthermore the outcomes of combined tests with an optical instrument are reported.

Investigation and Prediction of RF Window Performance in APT Accelerators

DOE Office of Scientific and Technical Information (OSTI.GOV)

Humphries, S. Jr.

1997-05-01

The work described in this report was performed between November 1996 and May 1997 in support of the APT (Accelerator Production of Tritium) Program at Los Alamos National Laboratory. The goal was to write and to test computer programs for charged particle orbits in RF fields. The well-documented programs were written in portable form and compiled for standard personal computers for easy distribution to LANL researchers. They will be used in several APT applications including the following. Minimization of multipactor effects in the moderate {beta} superconducting linac cavities under design for the APT accelerator. Investigation of suppression techniques for electronmore » multipactoring in high-power RF feedthroughs. Modeling of the response of electron detectors for the protection of high power RF vacuum windows. In the contract period two new codes, Trak{_}RF and WaveSim, were completed and several critical benchmark etests were carried out. Trak{_}RF numerically tracks charged particle orbits in combined electrostatic, magnetostatic and electromagnetic fields. WaveSim determines frequency-domain RF field solutions and provides a key input to Trak{_}RF. The two-dimensional programs handle planar or cylindrical geometries. They have several unique characteristics.« less

Advancement Of Tritium Powered Betavoltaic Battery Systems FY16 EOY Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Staack, G.; Gaillard, J.; Hitchcock, D.

2016-10-12

The goal of this work is to increase the power output of tritium-powered betavoltaic batteries and investigate the change in power output and film resistance in real-time during tritium loading of adsorbent films. To this end, several tritium-compatible test vessels with the capability of measuring both the resistivity of a tritium trapping film and the power output of a betavoltaic device in-situ have been designed and fabricated using four electrically insulated feedthroughs in tritium-compatible load cells. Energy conversion devices were received from Widetronix, a betavoltaic manufacturing firm based in Ithaca, NY. Thin films were deposited on the devices and cappedmore » with palladium to facilitate hydrogen loading. Gold contacts were then deposited on top of the films to allow resistivity measurements of the film during hydrogen loading. Finally, the chips were wire bonded and installed in the test cells. The cells were then baked-out under vacuum and leak checked at temperature to reduce the chances of tritium leaks during loading. Following the bake-out, IV curves were measured to verify no internal wires were compromised, and the cells were delivered to Tritium for loading. Tritium loading is anticipated in October, 2017.« less

A study of dielectric breakdown along insulators surrounding conductors in liquid argon

DOE PAGES

Lockwitz, Sarah; Jostlein, Hans

2016-03-22

High voltage breakdown in liquid argon is an important concern in the design of liquid argon time projection chambers, which are often used as neutrino and dark matter detectors. We have made systematic measurements of breakdown voltages in liquid argon along insulators surrounding negative rod electrodes where the breakdown is initiated at the anode. The measurements were performed in an open cryostat filled with commercial grade liquid argon exposed to air, and not the ultra-pure argon required for electron drift. While not addressing all high voltage concerns in liquid argon, these measurements have direct relevance to the design of highmore » voltage feedthroughs especially for averting the common problem of flash-over breakdown. The purpose of these tests is to understand the effects of materials, of breakdown path length, and of surface topology for this geometry and setup. We have found that the only material-specific effects are those due to their permittivity. We have found that the breakdown voltage has no dependence on the length of the exposed insulator. Lastly, a model for the breakdown mechanism is presented that can help inform future designs.« less

High-power piezoelectric acoustic-electric power feedthru for metal walls

NASA Astrophysics Data System (ADS)

Bao, Xiaoqi; Biederman, Will; Sherrit, Stewart; Badescu, Mircea; Bar-Cohen, Yoseph; Jones, Christopher; Aldrich, Jack; Chang, Zensheu

2008-03-01

Piezoelectric acoustic-electric power feed-through devices transfer electric power wirelessly through a solid wall using elastic waves. This approach allows for the elimination of the need for holes through structures for cabling or electrical feed-thrus . The technology supplies power to electric equipment inside sealed containers, vacuum or pressure vessels, etc where holes in the wall are prohibitive or may result in significant performance degradation or requires complex designs. In the our previous work, 100-W of electric power was transferred through a metal wall by a small, piezoelectric device with a simple-structure. To meet requirements of higher power applications, the feasibility to transfer kilowatts level power was investigated. Pre-stressed longitudinal piezoelectric feed-thru devices were analyzed by finite element modeling. An equivalent circuit model was developed to predict the characteristics of power transfer to different electric loads. Based on the analytical results, a prototype device was designed, fabricated and successfully demonstrated to transfer electric power at a level of 1-kW. Methods of minimizing plate wave excitation on the wall were also analyzed. Both model analysis and experimental results are presented in detail in this paper.

Web-Based, Participant-Driven Studies Yield Novel Genetic Associations for Common Traits

PubMed Central

Eriksson, Nicholas; Macpherson, J. Michael; Tung, Joyce Y.; Hon, Lawrence S.; Naughton, Brian; Saxonov, Serge; Avey, Linda; Wojcicki, Anne; Pe'er, Itsik; Mountain, Joanna

2010-01-01

Despite the recent rapid growth in genome-wide data, much of human variation remains entirely unexplained. A significant challenge in the pursuit of the genetic basis for variation in common human traits is the efficient, coordinated collection of genotype and phenotype data. We have developed a novel research framework that facilitates the parallel study of a wide assortment of traits within a single cohort. The approach takes advantage of the interactivity of the Web both to gather data and to present genetic information to research participants, while taking care to correct for the population structure inherent to this study design. Here we report initial results from a participant-driven study of 22 traits. Replications of associations (in the genes OCA2, HERC2, SLC45A2, SLC24A4, IRF4, TYR, TYRP1, ASIP, and MC1R) for hair color, eye color, and freckling validate the Web-based, self-reporting paradigm. The identification of novel associations for hair morphology (rs17646946, near TCHH; rs7349332, near WNT10A; and rs1556547, near OFCC1), freckling (rs2153271, in BNC2), the ability to smell the methanethiol produced after eating asparagus (rs4481887, near OR2M7), and photic sneeze reflex (rs10427255, near ZEB2, and rs11856995, near NR2F2) illustrates the power of the approach. PMID:20585627

Tunable electronic properties of partially edge-hydrogenated armchair boron-nitrogen-carbon nanoribbons.

PubMed

Alaal, Naresh; Medhekar, Nikhil; Shukla, Alok

2018-04-18

We employ a first-principles calculations based density-functional-theory (DFT) approach to study the electronic properties of partially and fully edge-hydrogenated armchair boron-nitrogen-carbon (BNC) nanoribbons (ABNCNRs), with widths between 0.85 nm to 2.3 nm. Due to the partial passivation of edges, the electrons, which do not participate in the bonding, form new energy states located near the Fermi-level. Because of these additional bands, some ABNCNRs exhibit metallic behavior, which is quite uncommon in armchair nanoribbons. Our calculations reveal that metallic behavior is observed for the following passivation patterns: (i) when the B atom from one edge and the N atom from another edge are unpassivated. (ii) when the N atoms from both the edges are unpassivated. (iii) when the C atom from one edge and the N atom from another edge are unpassivated. Furthermore, spin-polarization is also observed for certain passivation schemes, which is also quite uncommon for armchair nanoribbons. Thus, our results suggest that the ABNCNRs exhibit a wide range of electronic and magnetic properties in that the fully edge-hydrogenated ABNCNRs are direct band gap semiconductors, while the partially edge-hydrogenated ones are either semiconducting, or metallic, while simultaneously exhibiting spin polarization, based on the nature of passivation. We also find that the ribbons with larger widths are more stable as compared to the narrower ones.

A Real-Time Imaging System for Stereo Atomic Microscopy at SPring-8's BL25SU

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matsushita, Tomohiro; Guo, Fang Zhun; Muro, Takayuki

2007-01-19

We have developed a real-time photoelectron angular distribution (PEAD) and Auger-electron angular distribution (AEAD) imaging system at SPring-8 BL25SU, Japan. In addition, a real-time imaging system for circular dichroism (CD) studies of PEAD/AEAD has been newly developed. Two PEAD images recorded with left- and right-circularly polarized light can be regarded as a stereo image of the atomic arrangement. A two-dimensional display type mirror analyzer (DIANA) has been installed at the beamline, making it possible to record PEAD/AEAD patterns with an acceptance angle of {+-}60 deg. in real-time. The twin-helical undulators at BL25SU enable helicity switching of the circularly polarized lightmore » at 10Hz, 1Hz or 0.1Hz. In order to realize real-time measurements of the CD of the PEAD/AEAD, the CCD camera must be synchronized to the switching frequency. The VME computer that controls the ID is connected to the measurement computer with two BNC cables, and the helicity information is sent using TTL signals. For maximum flexibility, rather than using a hardware shutter synchronizing with the TTL signal we have developed software to synchronize the CCD shutter with the TTL signal. We have succeeded in synchronizing the CCD camera in both the 1Hz and 0.1Hz modes.« less

49 CFR 1242.27 - Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 9 2014-10-01 2014-10-01 false Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor vehicle loading and distribution facilities, and... Structures § 1242.27 Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals...

49 CFR 1242.27 - Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 9 2013-10-01 2013-10-01 false Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor vehicle loading and distribution facilities, and... Structures § 1242.27 Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals...

49 CFR 1242.27 - Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 9 2012-10-01 2012-10-01 false Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor vehicle loading and distribution facilities, and... Structures § 1242.27 Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals...

49 CFR 1242.27 - Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 9 2011-10-01 2011-10-01 false Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor vehicle loading and distribution facilities, and... Structures § 1242.27 Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals...

49 CFR 1242.27 - Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 9 2010-10-01 2010-10-01 false Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals, motor vehicle loading and distribution facilities, and... Structures § 1242.27 Coal marine terminals, ore marine terminals, TOFC/COFC terminals, other marine terminals...

Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET.

PubMed

Clerte, Caroline; Declerck, Nathalie; Margeat, Emmanuel

2013-02-01

The control of transcription termination by RNA-binding proteins that modulate RNA-structures is an important regulatory mechanism in bacteria. LicT and SacY from Bacillus subtilis prevent the premature arrest of transcription by binding to an anti-terminator RNA hairpin that overlaps an intrinsic terminator located in the 5'-mRNA leader region of the gene to be regulated. In order to investigate the molecular determinants of this anti-termination/termination balance, we have developed a fluorescence-based nucleic acids system that mimics the competition between the LicT or SacY anti-terminator targets and the overlapping terminators. Using Förster Resonance Energy Transfer on single diffusing RNA hairpins, we could monitor directly their opening or closing state, and thus investigate the effects on this equilibrium of the binding of anti-termination proteins or terminator-mimicking oligonucleotides. We show that the anti-terminator hairpins adopt spontaneously a closed structure and that their structural dynamics is mainly governed by the length of their basal stem. The induced stability of the anti-terminator hairpins determines both the affinity and specificity of the anti-termination protein binding. Finally, we show that stabilization of the anti-terminator hairpin, by an extended basal stem or anti-termination protein binding can efficiently counteract the competing effect of the terminator-mimic.

Measurement and modeling of intrinsic transcription terminators

PubMed Central

Cambray, Guillaume; Guimaraes, Joao C.; Mutalik, Vivek K.; Lam, Colin; Mai, Quynh-Anh; Thimmaiah, Tim; Carothers, James M.; Arkin, Adam P.; Endy, Drew

2013-01-01

The reliable forward engineering of genetic systems remains limited by the ad hoc reuse of many types of basic genetic elements. Although a few intrinsic prokaryotic transcription terminators are used routinely, termination efficiencies have not been studied systematically. Here, we developed and validated a genetic architecture that enables reliable measurement of termination efficiencies. We then assembled a collection of 61 natural and synthetic terminators that collectively encode termination efficiencies across an ∼800-fold dynamic range within Escherichia coli. We simulated co-transcriptional RNA folding dynamics to identify competing secondary structures that might interfere with terminator folding kinetics or impact termination activity. We found that structures extending beyond the core terminator stem are likely to increase terminator activity. By excluding terminators encoding such context-confounding elements, we were able to develop a linear sequence-function model that can be used to estimate termination efficiencies (r = 0.9, n = 31) better than models trained on all terminators (r = 0.67, n = 54). The resulting systematically measured collection of terminators should improve the engineering of synthetic genetic systems and also advance quantitative modeling of transcription termination. PMID:23511967

14 CFR 1260.161 - Termination.

Code of Federal Regulations, 2011 CFR

2011-01-01

..., Hospitals, and Other Non-Profit Organizations Termination and Enforcement § 1260.161 Termination. (a) Awards... termination conditions, including the effective date and, in the case of partial termination, the portion to... reasons for such termination, the effective date, and, in the case of partial termination, the portion to...

Through-Metal-Wall Power Delivery and Data Transmission for Enclosed Sensors: A Review

PubMed Central

Yang, Ding-Xin; Hu, Zheng; Zhao, Hong; Hu, Hai-Feng; Sun, Yun-Zhe; Hou, Bao-Jian

2015-01-01

The aim of this review was to assess the current viable technologies for wireless power delivery and data transmission through metal barriers. Using such technologies sensors enclosed in hermetical metal containers can be powered and communicate through exterior power sources without penetration of the metal wall for wire feed-throughs. In this review, we first discuss the significant and essential requirements for through-metal-wall power delivery and data transmission and then we: (1) describe three electromagnetic coupling based techniques reported in the literature, which include inductive coupling, capacitive coupling, and magnetic resonance coupling; (2) present a detailed review of wireless ultrasonic through-metal-wall power delivery and/or data transmission methods; (3) compare various ultrasonic through-metal-wall systems in modeling, transducer configuration and communication mode with sensors; (4) summarize the characteristics of electromagnetic-based and ultrasound-based systems, evaluate the challenges and development trends. We conclude that electromagnetic coupling methods are suitable for through thin non-ferromagnetic metal wall power delivery and data transmission at a relatively low data rate; piezoelectric transducer-based ultrasonic systems are particularly advantageous in achieving high power transfer efficiency and high data rates; the combination of more than one single technique may provide a more practical and reliable solution for long term operation. PMID:26694392

Wireless Strain Measurement with a Micromachined Magnetoelastic Resonator Using Ringdown Frequency Locking

PubMed Central

Green, Scott R.; Gianchandani, Yogesh B.

2017-01-01

Resonant magnetoelastic devices are widely used as anti-theft tags and are also being investigated for a range of sensing applications. The vast majority of magnetoelastic devices are operated at resonance, and rely upon an external interface to wirelessly detect the resonant frequency, and other characteristics. For micromachined devices, this detection method must accommodate diminished signal strength and elevated resonant frequencies. Feedthrough of the interrogating stimulus to the detector also presents a significant challenge. This paper describes a method of interrogating wireless magnetoelastic strain sensors using a new frequency-lock approach. Following a brief excitation pulse, the sensor ring-down is analyzed and a feedback loop is used to match the excitation frequency and the resonant frequency. Data acquisition hardware is used in conjunction with custom software to implement the frequency-lock loop. Advantages of the method include temporal isolation of interrogating stimulus from the sensor response and near real-time tracking of resonant frequencies. The method was investigated using a family of wireless strain sensors with resonant frequencies ranging from 120 to 240 kHz. Strain levels extending to 3.5 mstrain and sensitivities up to 14300 ppm/mstrain were measured with response times faster than 0.5 s. The standard deviation of the locked frequency did not exceed 0.1%. PMID:28713873

Space-Qualified Traveling-Wave Tube

NASA Technical Reports Server (NTRS)

Wilson, Jeffrey D.; Krawczyk, Richard; Simons, Rainee N.; Williams, Wallace D.; Robbins, Neal R.; Dibb, Daniel R.; Menninger, William L.; Zhai, Xiaoling; Benton, Robert T.

2010-01-01

The L-3 Communications Electron Technologies, Inc. Model 999HA traveling-wave tube (TWT), was developed for use as a high-power microwave amplifier for high-rate transmission of data and video signals from deep space to Earth (see figure). The 999HA is a successor to the 999H a non-space qualified TWT described in High-Power, High-Efficiency Ka-Band Traveling-Wave Tube (LEW-17900-1), NASA Tech Briefs, Vol. 31, No. 2 (February 2007), page 32. Operating in the 31.8-to-32.3 GHz frequency band, the 999HA has been shown to generate 252 W of continuous- wave output power at 62 percent overall power efficiency a 75-percent increase in output power over the 999H. The mass of the 999HA is 35 percent less than that of the 999H. Moreover, taking account of the elimination of a Faraday cage that is necessary for operation of the 999H but is obviated by a redesign of high-voltage feed-throughs for the 999HA, the overall reduction in mass becomes 57 percent with an 82 percent reduction in volume. Through a series of rigorous tests, the 999HA has been qualified for operation aboard spacecraft with a lifetime exceeding seven years. Offspring of the 999HA will fly on the Kepler and Lunar Reconnaissance Orbiter missions.

Toward increased reliability in the electric power industry: direct temperature measurement in transformers using fiber optic sensors

NASA Astrophysics Data System (ADS)

McDonald, Greg

1998-09-01

Optimal loading, prevention of catastrophic failures and reduced maintenance costs are some of the benefits of accurate determination of hot spot winding temperatures in medium and high power transformers. Temperature estimates obtained using current theoretical models are not always accurate. Traditional technology (IR, thermocouples...) are unsuitable or inadequate for direct measurement. Nortech fiber-optic temperature sensors offer EMI immunity and chemical resistance and are a proven solution to the problem. The Nortech sensor's measurement principle is based on variations in the spectral absorption of a fiber-mounted semiconductor chip and probes are interchangeable with no need for recalibration. Total length of probe + extension can be up to several hundred meters allowing system electronics to be located in the control room or mounted in the transformer instrumentation cabinet. All of the sensor materials withstand temperatures up to 250 degree(s)C and have demonstrated excellent resistance to the harsh transformer environment (hot oil, kerosene). Thorough study of the problem and industry collaboration in testing and installation allows Nortech to identify and meet the need for durable probes, leak-proof feedthroughs, standard computer interfaces and measurement software. Refined probe technology, the method's simplicity and reliable calibration are all assets that should lead to growing acceptance of this type of direct measuring in the electric power industry.

Technical Capability Upgrades to the NASA Langley Research Center 8 ft. by 15 ft. Thermal Vacuum Chamber

NASA Technical Reports Server (NTRS)

Thornblom, Mark N.; Beverly, Joshua; O'Connell, Joseph J.; Duncan, Dwight L.

2016-01-01

The 8 ft. by 15 ft. thermal vacuum chamber (TVAC), housed in Building 1250 at the NASA Langley Research Center (LaRC), and managed by the Systems Integration and Test Branch within the Engineering Directorate, has undergone several significant modifications to increase testing capability, safety, and quality of measurements of articles under environmental test. Significant modifications include: a new nitrogen distribution manifold for supplying the shroud and other cold surfaces to liquid nitrogen temperatures; a new power supply and distribution system for accurately controlling a quartz IR lamp suite; a suite of contamination monitoring sensors for outgassing measurements and species identification; a new test article support system; signal and power feed-throughs; elimination of unnecessary penetrations; and a new data acquisition and control commanding system including safety interlocks. This paper will provide a general overview of the LaRC 8 ft. by 15 ft. TVAC chamber, an overview of the new technical capabilities, and will illustrate each upgrade in detail, in terms of mechanical design and predicted performance. Additionally, an overview of the scope of tests currently being performed in the chamber will be documented, and sensor plots from tests will be provided to show chamber temperature and pressure performance with actual flight hardware under test.

Wafer-level vacuum/hermetic packaging technologies for MEMS

NASA Astrophysics Data System (ADS)

Lee, Sang-Hyun; Mitchell, Jay; Welch, Warren; Lee, Sangwoo; Najafi, Khalil

2010-02-01

An overview of wafer-level packaging technologies developed at the University of Michigan is presented. Two sets of packaging technologies are discussed: (i) a low temperature wafer-level packaging processes for vacuum/hermeticity sealing, and (ii) an environmentally resistant packaging (ERP) technology for thermal and mechanical control as well as vacuum packaging. The low temperature wafer-level encapsulation processes are implemented using solder bond rings which are first patterned on a cap wafer and then mated with a device wafer in order to encircle and encapsulate the device at temperatures ranging from 200 to 390 °C. Vacuum levels below 10 mTorr were achieved with yields in an optimized process of better than 90%. Pressures were monitored for more than 4 years yielding important information on reliability and process control. The ERP adopts an environment isolation platform in the packaging substrate. The isolation platform is designed to provide low power oven-control, vibration isolation and shock protection. It involves batch flip-chip assembly of a MEMS device onto the isolation platform wafer. The MEMS device and isolation structure are encapsulated at the wafer-level by another substrate with vertical feedthroughs for vacuum/hermetic sealing and electrical signal connections. This technology was developed for high performance gyroscopes, but can be applied to any type of MEMS device.

Localized heating/bonding techniques in MEMS packaging

NASA Astrophysics Data System (ADS)

Mabesa, J. R., Jr.; Scott, A. J.; Wu, X.; Auner, G. W.

2005-05-01

Packaging is used to protect and enable intelligent sensor systems utilized in manned/unmanned ground vehicle systems/subsystems. Because Micro electro mechanical systems (MEMS) are used often in these sensor or actuation products, it must interact with the surrounding environment, which may be in direct conflict with the desire to isolate the electronics for improved reliability/durability performance. For some very simple devices, performance requirements may allow a high degree of isolation from the environment (e.g., stints and accelerometers). Other more complex devices (i.e. chemical and biological analysis systems, particularly in vivo systems) present extremely complex packaging requirements. Power and communications to MEMS device arrays are also extremely problematic. The following describes the research being performed at the U.S. Army Research, Development, and Engineering Command (RDECOM) Tank and Automotive Research, Development, and Engineering Center (TARDEC), in collaboration with Wayne State University, in Detroit, MI. The focus of the packaging research is limited to six main categories: a) provision for feed-through for electrical, optical, thermal, and fluidic interfaces; b) environmental management including atmosphere, hermiticity, and temperature; c) control of stress and mechanical durability; d) management of thermal properties to minimize absorption and/or emission; e) durability and structural integrity; and f) management of RF/magnetic/electrical and optical interference and/or radiation properties and exposure.

Technical Capability Upgrades to the NASA Langley Research Center 6 ft. by 6 ft. Thermal Vacuum Chamber

NASA Technical Reports Server (NTRS)

Thornblom, Mark N.; Beverly, Joshua; O'Connell, Joseph J.; Mau, Johnny C.; Duncan, Dwight L.

2014-01-01

The 6 ft. by 6 ft. thermal vacuum chamber (TVAC), housed in Building 1250 at the NASA Langley Research Center (LaRC), and managed by the Systems Integration and Test Branch within the Engineering Directorate, has undergone several significant modifications to increase testing capability, safety, and quality of measurements of articles under environmental test. Significant modifications include: a new nitrogen thermal conditioning unit for controlling shroud temperatures from -150degC to +150degC; two horizontal auxiliary cold plates for independent temperature control from -150degC to +200degC; a suite of contamination monitoring sensors for outgassing measurements and species identification; signal and power feed-throughs; new pressure gauges; and a new data acquisition and control commanding system including safety interlocks. This presentation will provide a general overview of the LaRC 6 ft. by 6 ft. TVAC chamber, an overview of the new technical capabilities, and illustrate each upgrade in detail, in terms of mechanical design and predicted performance. Additionally, an overview of the scope of tests currently being performed in the chamber will be documented, and sensor plots from tests will be provided to show chamber temperature and pressure performance with actual flight hardware under test.

Numerical simulations of Richtmyer{endash}Meshkov instabilities in finite-thickness fluid layers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mikaelian, K.O.

1996-05-01

Direct numerical simulations of Richtmyer{endash}Meshkov instabilities in shocked fluid layers are reported and compared with analytic theory. To investigate new phenomena such as freeze-out, interface coupling, and feedthrough, several new configurations are simulated on a two-dimensional hydrocode. The basic system is an {ital A}/{ital B}/{ital A} combination, where {ital A} is air and {ital B} is a finite-thickness layer of freon, SF{sub 6}, or helium. The middle layer {ital B} has perturbations either on its upstream or downstream side, or on both sides, in which case the perturbations may be in phase (sinuous) or out of phase (varicose). The evolutionmore » of such perturbations under a Mach 1.5 shock is calculated, including the effect of a reshock. Recently reported gas curtain experiments [J. M. Budzinski {ital et} {ital al}., Phys. Fluids {bold 6}, 3510 (1994)] are also simulated and the code results are found to agree very well with the experiments. A new gas curtain configuration is also considered, involving an initially sinuous SF{sub 6} or helium layer and a new pattern, opposite mushrooms, is predicted to emerge. Upon reshock a relatively simple sinuous gas curtain is found to evolve into a highly complex pattern of nested mushrooms. {copyright} {ital 1996 American Institute of Physics.}« less

Characterization facility for magneto-optic media and systems

NASA Technical Reports Server (NTRS)

Mansuripur, M.; Fu, H.; Gadetsky, S.; Sugaya, S.; Wu, T. H.; Zambuto, J.; Gerber, R.; Goodman, T.; Erwin, J. K.

1993-01-01

Objectives of this research are: (1) to measure the hysteresis loop, Kerr rotation angle, anisotropy energy profile, Hall voltage, and magnetoresistance of thin-film magneto-optic media using our loop-tracer; (2) measure the wavelength-dependence of the Kerr rotation angle, Theta(sub k), and ellipticity, epsilon(sub k), for thin-film media using our magneto-optic Kerr spectrometer (MOKS); (3) measure the dielectric tensor of thin-film and multilayer samples using our variable-angle magneto-optic ellipsometer (VAMOE); (4) measure the hysteresis loop, coercivity, remanent magnetization, saturation magnetization, and anisotropy energy constant for thin film magnetic media using vibrating sample magnetometry; (5) observe small magnetic domains and investigate their interaction with defects using magnetic force microscopy; (6) perform static read/write/erase experiments on thin-film magneto-optic media using our static test station; (7) integrate the existing models of magnetization, magneto-optic effects, coercivity, and anisotropy in an interactive and user-friendly environment, and analyze the characterization data obtained in the various experiments, using this modeling package; (8) measure focusing- and tracking-error signals on a static testbed, determine the 'feedthrough' for various focusing schemes, investigate the effects of polarization and birefringence, and compare the results with diffraction-based calculations; and (9) measure the birefringence of optical disk substrates using two variable angle ellipsometers.

Evaluation of inlet sampling integrity on NSF/NCAR airborne platforms

NASA Astrophysics Data System (ADS)

Campos, T. L.; Stith, J. L.; Stephens, B. B.; Romashkin, P.

2017-12-01

An inlet test project was conducted during IDEAS-IV-GV (2013), to evaluate the sampling integrity of two inlet designs. Use of a single CO2 sensor provided a high precision detector and a large difference in the mean cabin and external concentrations (500-700 ppmv in the cabin). The original HIAPER Modular InLet (HIMIL) is comprised of a tapered flow straightening flow through `cigar' mounted to a strut. The cigar center sampling line sits 12" from the fuselage skin. An o-ring seals the feedthrough plate coupling sampling lines from the strut into the cigar. However, there is no seal to prevent air inside the strut from seeping out around the cigar body. A pressure-equalizing drain hole in the strut access panel; it was positioned at an approximate distance of 4" from the fuselage to ensure that air from any source that drained out of the strut was confined to a low release point. A second aft-facing inlet design was also evaluated. The sampling center line was moved farther from the fuselage at a height of 16". A similar approach was also applied to sampling locations on the C-130 in 2015. The results of these tests and recommendations for best practices will be presented.

Poly-Si TFTs integrated gate driver circuit with charge-sharing structure

NASA Astrophysics Data System (ADS)

Chen, Meng; Lei, Jiefeng; Huang, Shengxiang; Liao, Congwei; Deng, Lianwen

2017-06-01

A p-type low-temperature poly-Si thin film transistors (LTPS TFTs) integrated gate driver using 2 non-overlapped clocks is proposed. This gate driver features charge-sharing structure to turn off buffer TFT and suppresses voltage feed-through effects. It is analyzed that the conventional gate driver suffers from waveform distortions due to voltage uncertainty of internal nodes for the initial period. The proposed charge-sharing structure also helps to suppress the unexpected pulses during the initialization phases. The proposed gate driver shows a simple circuit, as only 6 TFTs and 1 capacitor are used for single-stage, and the buffer TFT is used for both pulling-down and pulling-up of output electrode. Feasibility of the proposed gate driver is proven through detailed analyses. Investigations show that voltage bootrapping can be maintained once the bootrapping capacitance is larger than 0.8 pF, and pulse of gate driver outputs can be reduced to 5 μs. The proposed gate driver can still function properly with positive {V}{TH} shift within 0.4 V and negative {V}{TH} shift within -1.2 V and it is robust and promising for high-resolution display. Project supported by the Science and Technology Project of Hunan Province, China (No. 2015JC3401)

STUDY OF THE RHIC BPM SMA CONNECTOR FAILURE PROBLEM

DOE Office of Scientific and Technical Information (OSTI.GOV)

LIAW,C.; SIKORA, R.; SCHROEDER, R.

2007-06-25

About 730 BPMs are mounted on the RHIC CQS and Triplet super-conducting magnets. Semi-rigid coaxial cables are used to bring the electrical signal from the BPM feedthroughs to the outside flanges. at the ambient temperature. Every year around 10 cables will lose their signals during the operation. The connection usually failed at the warm end of the cable. The problems were either the solder joint failed or the center conductor retracted out of the SMA connector. Finite element analyses were performed to understand the failure mechanism of the solder joint. The results showed that (1) The SMA center conductor canmore » separate from the mating connector due to the thermal retraction. (2) The maximum thermal stress at the warm end solder joint can exceed the material strength of the Pb37/Sn63 solder material and (3) The magnet ramping frequency (-10 Hz), during the machine startup, can possibly resonant the coaxial cable and damage the solder joints, especially when a fracture is initiated. Test results confirmed that by using the silver bearing solder material (a higher strength material) and by crimping the cable at the locations close to the SMA connector (to prevent the center conductor from retracting) can effectively resolve the connector failure problem.« less

Self-contained in-vacuum in situ thin film stress measurement tool

NASA Astrophysics Data System (ADS)

Reinink, J.; van de Kruijs, R. W. E.; Bijkerk, F.

2018-05-01

A fully self-contained in-vacuum device for measuring thin film stress in situ is presented. The stress was measured by measuring the curvature of a cantilever on which the thin film was deposited. For this, a dual beam laser deflectometer was used. All optics and electronics needed to perform the measurement are placed inside a vacuum-compatible vessel with the form factor of the substrate holders of the deposition system used. The stand-alone nature of the setup allows the vessel to be moved inside a deposition system independently of optical or electronic feedthroughs while measuring continuously. A Mo/Si multilayer structure was analyzed to evaluate the performance of the setup. A radius of curvature resolution of 270 km was achieved. This allows small details of the stress development to be resolved, such as the interlayer formation between the layers and the amorphous-to-crystalline transition of the molybdenum which occurs at around 2 nm. The setup communicates with an external computer via a Wi-Fi connection. This wireless connection allows remote control over the acquisition and the live feedback of the measured stress. In principle, the vessel can act as a general metrology platform and add measurement capabilities to deposition setups with no modification to the deposition system.

On the mechanisms of interference between mobile phones and pacemakers: parasitic demodulation of GSM signal by the sensing amplifier.

PubMed

Barbaro, V; Bartolini, P; Calcagnini, G; Censi, F; Beard, B; Ruggera, P; Witters, D

2003-06-07

The aim of this study was to investigate the mechanisms by which the radiated radiofrequency (RF) GSM (global system for mobile communication) signal may affect pacemaker (PM) function. We measured the signal at the output of the sensing amplifier of PMs with various configurations of low-pass filters. We used three versions of the same PM model: one with a block capacitor which short circuits high-frequency signals; one with a ceramic feedthrough capacitor, a hermetically sealed mechanism connecting the internal electronics to the external connection block, and one with both. The PMs had been modified to have an electrical shielded connection to the output of the sensing amplifier. For each PM, the output of the sensing amplifier was monitored under exposure to modulated and non-modulated RF signals, and to GSM signals (900 and 1800 MHz). Non-modulated RF signals did not alter the response of the PM sensing amplifier. Modulated RF signals showed that the block capacitor did not succeed in short circuiting the RF signal, which is somehow demodulated by the PM internal non-linear circuit elements. Such a demodulation phenomenon poses a critical problem because digital cellular phones use extremely low-frequency modulation (as low as 2 Hz). which can be mistaken for normal heartbeat.

Model reduction and frequency residuals for a robust estimation of nonlinearities in subspace identification

NASA Astrophysics Data System (ADS)

De Filippis, G.; Noël, J. P.; Kerschen, G.; Soria, L.; Stephan, C.

2017-09-01

The introduction of the frequency-domain nonlinear subspace identification (FNSI) method in 2013 constitutes one in a series of recent attempts toward developing a realistic, first-generation framework applicable to complex structures. If this method showed promising capabilities when applied to academic structures, it is still confronted with a number of limitations which needs to be addressed. In particular, the removal of nonphysical poles in the identified nonlinear models is a distinct challenge. In the present paper, it is proposed as a first contribution to operate directly on the identified state-space matrices to carry out spurious pole removal. A modal-space decomposition of the state and output matrices is examined to discriminate genuine from numerical poles, prior to estimating the extended input and feedthrough matrices. The final state-space model thus contains physical information only and naturally leads to nonlinear coefficients free of spurious variations. Besides spurious variations due to nonphysical poles, vibration modes lying outside the frequency band of interest may also produce drifts of the nonlinear coefficients. The second contribution of the paper is to include residual terms, accounting for the existence of these modes. The proposed improved FNSI methodology is validated numerically and experimentally using a full-scale structure, the Morane-Saulnier Paris aircraft.

Air Pressure Controlled Mass Measurement System

NASA Astrophysics Data System (ADS)

Zhong, Ruilin; Wang, Jian; Cai, Changqing; Yao, Hong; Ding, Jin'an; Zhang, Yue; Wang, Xiaolei

Mass measurement is influenced by air pressure, temperature, humidity and other facts. In order to reduce the influence, mass laboratory of National Institute of Metrology, China has developed an air pressure controlled mass measurement system. In this system, an automatic mass comparator is installed in an airtight chamber. The Chamber is equipped with a pressure controller and associate valves, thus the air pressure can be changed and stabilized to the pre-set value, the preferred pressure range is from 200 hPa to 1100 hPa. In order to keep the environment inside the chamber stable, the display and control part of the mass comparator are moved outside the chamber, and connected to the mass comparator by feed-throughs. Also a lifting device is designed for this system which can easily lift up the upper part of the chamber, thus weights can be easily put inside the mass comparator. The whole system is put on a marble platform, and the temperature and humidity of the laboratory is very stable. The temperature, humidity, and carbon dioxide content inside the chamber are measured in real time and can be used to get air density. Mass measurement cycle from 1100 hPa to 200 hPa and back to 1100 hPa shows the effective of the system.

A genetic variant in the seed region of miR-4513 shows pleiotropic effects on lipid and glucose homeostasis, blood pressure, and coronary artery disease.

PubMed

Ghanbari, Mohsen; de Vries, Paul S; de Looper, Hans; Peters, Marjolein J; Schurmann, Claudia; Yaghootkar, Hanieh; Dörr, Marcus; Frayling, Timothy M; Uitterlinden, Andre G; Hofman, Albert; van Meurs, Joyce B J; Erkeland, Stefan J; Franco, Oscar H; Dehghan, Abbas

2014-12-01

MicroRNAs (miRNA) play a crucial role in the regulation of diverse biological processes by post-transcriptional modulation of gene expression. Genetic polymorphisms in miRNA-related genes can potentially contribute to a wide range of phenotypes. The effect of such variants on cardiometabolic diseases has not yet been defined. We systematically investigated the association of genetic variants in the seed region of miRNAs with cardiometabolic phenotypes, using the thus far largest genome-wide association studies on 17 cardiometabolic traits/diseases. We found that rs2168518:G>A, a seed region variant of miR-4513, associates with fasting glucose, low-density lipoprotein-cholesterol, total cholesterol, systolic and diastolic blood pressure, and risk of coronary artery disease. We experimentally showed that miR-4513 expression is significantly reduced in the presence of the rs2168518 mutant allele. We sought to identify miR-4513 target genes that may mediate these associations and revealed five genes (PCSK1, BNC2, MTMR3, ANK3, and GOSR2) through which these effects might be taking place. Using luciferase reporter assays, we validated GOSR2 as a target of miR-4513 and further demonstrated that the miRNA-mediated regulation of this gene is changed by rs2168518. Our findings indicate a pleiotropic effect of miR-4513 on cardiometabolic phenotypes and may improve our understanding of the pathophysiology of cardiometabolic diseases. © 2014 WILEY PERIODICALS, INC.

Comparison of GEANT4 Physics Models with Measured Beta Particle Data in Aluminum using a Strontium-90 Source

NASA Astrophysics Data System (ADS)

Everett, Samantha

2010-10-01

A transmission curve experiment was carried out to measure the range of beta particles in aluminum in the health physics laboratory located on the campus of Texas Southern University. The transmission count rate through aluminum for varying radiation lengths was measured using beta particles emitted from a low activity (˜1 μCi) Sr-90 source. The count rate intensity was recorded using a Geiger Mueller tube (SGC N210/BNC) with an active volume of 61 cm^3 within a systematic detection accuracy of a few percent. We compared these data with a realistic simulation of the experimental setup using the Geant4 Monte Carlo toolkit (version 9.3). The purpose of this study was to benchmark our Monte Carlo for future experiments as part of a more comprehensive research program. Transmission curves were simulated based on the standard and low-energy electromagnetic physics models, and using the radioactive decay module for the electrons primary energy distribution. To ensure the validity of our measurements, linear extrapolation techniques were employed to determine the in-medium beta particle range from the measured data and was found to be 1.87 g/cm^2 (˜0.693 cm), in agreement with literature values. We found that the general shape of the measured data and simulated curves were comparable; however, a discrepancy in the relative count rates was observed. The origin of this disagreement is still under investigation.

Energy efficiency in wireless communication systems

DOEpatents

Caffrey, Michael Paul; Palmer, Joseph McRae

2012-12-11

Wireless communication systems and methods utilize one or more remote terminals, one or more base terminals, and a communication channel between the remote terminal(s) and base terminal(s). The remote terminal applies a direct sequence spreading code to a data signal at a spreading factor to provide a direct sequence spread spectrum (DSSS) signal. The DSSS signal is transmitted over the communication channel to the base terminal which can be configured to despread the received DSSS signal by a spreading factor matching the spreading factor utilized to spread the data signal. The remote terminal and base terminal can dynamically vary the matching spreading factors to adjust the data rate based on an estimation of operating quality over time between the remote terminal and base terminal such that the amount of data being transmitted is substantially maximized while providing a specified quality of service.

Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET

PubMed Central

Clerte, Caroline; Declerck, Nathalie; Margeat, Emmanuel

2013-01-01

The control of transcription termination by RNA-binding proteins that modulate RNA-structures is an important regulatory mechanism in bacteria. LicT and SacY from Bacillus subtilis prevent the premature arrest of transcription by binding to an anti-terminator RNA hairpin that overlaps an intrinsic terminator located in the 5′-mRNA leader region of the gene to be regulated. In order to investigate the molecular determinants of this anti-termination/termination balance, we have developed a fluorescence-based nucleic acids system that mimics the competition between the LicT or SacY anti-terminator targets and the overlapping terminators. Using Förster Resonance Energy Transfer on single diffusing RNA hairpins, we could monitor directly their opening or closing state, and thus investigate the effects on this equilibrium of the binding of anti-termination proteins or terminator-mimicking oligonucleotides. We show that the anti-terminator hairpins adopt spontaneously a closed structure and that their structural dynamics is mainly governed by the length of their basal stem. The induced stability of the anti-terminator hairpins determines both the affinity and specificity of the anti-termination protein binding. Finally, we show that stabilization of the anti-terminator hairpin, by an extended basal stem or anti-termination protein binding can efficiently counteract the competing effect of the terminator-mimic. PMID:23303779

Client-therapist agreement in the termination process and its association with therapeutic relationship.

PubMed

Olivera, Julieta; Challú, Laura; Gómez Penedo, Juan Martín; Roussos, Andrés

2017-03-01

There is no consensus among different therapeutic approaches on the process of termination when therapy does not have a prefixed duration. Moreover, both clinicians and researchers are still exploring decision making in the termination of treatment. The present study assessed former client's perspective of therapy termination in a nonprobabilistic sample from Buenos Aires, Argentina. Seventy-three semistructured interviews, lasting ∼60 min each, were conducted with participants that had finished a therapeutic treatment or dropped out. They were asked about several aspects of therapy, including their experience of termination, specifically who decided to terminate, if there was agreement on termination or not, and their thoughts on the termination process. All interviews were transcribed and analyzed using an adaptation of Consensual Qualitative Research (CQR). Quantitative analyses were also conducted to examine associations between variables. Two main factors emerged from the analysis: client/therapist initiative on termination; and level of agreement between client and therapist regarding termination. Whereas nearly all (95%) of therapist-initiated termination cases agreed on termination, client-initiated termination cases could be sorted in agreed (49%) and disagreed (51%) terminations. Both therapist-initiated terminations and agreed upon terminations presented more categories of positive termination motives, better therapeutic bond, and higher overall satisfaction with treatment. Implications for research and clinical practice are discussed. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

Opposite consequences of two transcription pauses caused by an intrinsic terminator oligo(U): antitermination versus termination by bacteriophage T7 RNA polymerase.

PubMed

Lee, Sooncheol; Kang, Changwon

2011-05-06

The RNA oligo(U) sequence, along with an immediately preceding RNA hairpin structure, is an essential cis-acting element for bacterial class I intrinsic termination. This sequence not only causes a pause in transcription during the beginning of the termination process but also facilitates transcript release at the end of the process. In this study, the oligo(U) sequence of the bacteriophage T7 intrinsic terminator Tφ, rather than the hairpin structure, induced pauses of phage T7 RNA polymerase not only at the termination site, triggering a termination process, but also 3 bp upstream, exerting an antitermination effect. The upstream pause presumably allowed RNA to form a thermodynamically more stable secondary structure rather than a terminator hairpin and to persist because the 5'-half of the terminator hairpin-forming sequence could be sequestered by a farther upstream sequence via sequence-specific hybridization, prohibiting formation of the terminator hairpin and termination. The putative antiterminator RNA structure lacked several base pairs essential for termination when probed using RNases A, T1, and V1. When the antiterminator was destabilized by incorporation of IMP into nascent RNA at G residue positions, antitermination was abolished. Furthermore, antitermination strength increased with more stable antiterminator secondary structures and longer pauses. Thus, the oligo(U)-mediated pause prior to the termination site can exert a cis-acting antitermination activity on intrinsic terminator Tφ, and the termination efficiency depends primarily on the termination-interfering pause that precedes the termination-facilitating pause at the termination site.

46 CFR 525.2 - Terminal schedules.

Code of Federal Regulations, 2011 CFR

2011-10-01

... scrap, new assembled motor vehicles, waste paper and paper waste in terminal schedules. (2) Marine... MARITIME COMMISSION REGULATIONS AFFECTING OCEAN SHIPPING IN FOREIGN COMMERCE MARINE TERMINAL OPERATOR SCHEDULES § 525.2 Terminal schedules. (a) Marine terminal operator schedules. A marine terminal operator, at...

46 CFR 525.2 - Terminal schedules.

Code of Federal Regulations, 2010 CFR

2010-10-01

... scrap, new assembled motor vehicles, waste paper and paper waste in terminal schedules. (2) Marine... MARITIME COMMISSION REGULATIONS AFFECTING OCEAN SHIPPING IN FOREIGN COMMERCE MARINE TERMINAL OPERATOR SCHEDULES § 525.2 Terminal schedules. (a) Marine terminal operator schedules. A marine terminal operator, at...

Suppression of Factor-Dependent Transcription Termination by Antiterminator RNA

PubMed Central

King, Rodney A.; Weisberg, Robert A.

2003-01-01

Nascent transcripts of the phage HK022 put sites modify the transcription elongation complex so that it terminates less efficiently at intrinsic transcription terminators and accelerates through pause sites. We show here that the modification also suppresses termination in vivo at two factor-dependent terminators, one that depends on the bacterial Rho protein and a second that depends on the HK022-encoded Nun protein. Suppression was efficient when the termination factors were present at physiological levels, but an increase in the intracellular concentration of Nun increased termination both in the presence and absence of put. put-mediated antitermination thus shows no apparent terminator specificity, suggesting that put inhibits a step that is common to termination at the different types of terminator. PMID:14645267

Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator

PubMed Central

Li, Rui; Zhang, Qing; Li, Junbai; Shi, Hualin

2016-01-01

An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position–function relations were quantified for the terminator tR2 in Escherichia coli. The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE–distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology. PMID:26602687

Sequences required for transcription termination at the intrinsic lambdatI terminator.

PubMed

Martínez-Trujillo, Miguel; Sánchez-Trujillo, Alejandra; Ceja, Víctor; Avila-Moreno, Federico; Bermúdez-Cruz, Rosa María; Court, Donald; Montañez, Cecilia

2010-02-01

The lambdatI terminator is located approximately 280 bp beyond the lambdaint gene, and it has a typical structure of an intrinsic terminator. To identify sequences required for lambdatI transcription termination a set of deletion mutants were generated, either from the 5' or the 3' end onto the lambdatI region. The termination efficiency was determined by measuring galactokinase (galK) levels by Northern blot assays and by in vitro transcription termination. The importance of the uridines and the stability of the stem structure in the termination were demonstrated. The nontranscribed DNA beyond the 3' end also affects termination. Additionally, sequences upstream have a small effect on transcription termination. The in vivo RNA termination sites at lambdatI were determined by S1 mapping and were located at 8 different positions. Processing of transcripts from the 3' end confirmed the importance of the hairpin stem in protection against exonuclease.

10 CFR 600.161 - Termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Nonprofit Organizations Post-Award Requirements § 600.161 Termination. (a) Awards may be terminated in whole... effective date and, in the case of partial termination, the portion to be terminated. (3) By the recipient upon sending to DOE written notification setting forth the reasons for such termination, the effective...

10 CFR 600.161 - Termination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Nonprofit Organizations Post-Award Requirements § 600.161 Termination. (a) Awards may be terminated in whole... effective date and, in the case of partial termination, the portion to be terminated. (3) By the recipient upon sending to DOE written notification setting forth the reasons for such termination, the effective...

45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2014 CFR

2014-10-01

... NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8 Termination file and Examiner's report. (a) Preparation and Content... part of the termination file. (b) Review by Volunteer. On completion of the termination file, the...

45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2011 CFR

2011-10-01

... NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8 Termination file and Examiner's report. (a) Preparation and Content... part of the termination file. (b) Review by Volunteer. On completion of the termination file, the...

45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8 Termination file and Examiner's report. (a) Preparation and Content... part of the termination file. (b) Review by Volunteer. On completion of the termination file, the...

45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2013 CFR

2013-10-01

... NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8 Termination file and Examiner's report. (a) Preparation and Content... part of the termination file. (b) Review by Volunteer. On completion of the termination file, the...

78 FR 17890 - Energy Efficiency Program for Commercial and Industrial Equipment: Public Meeting and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-25

... Document for Packaged Terminal Air Conditioners and Packaged Terminal Heat Pumps AGENCY: Office of Energy... must identify the framework document for packaged terminal air conditioners and packaged terminal heat... packaged terminal air conditioners and packaged terminal heat pumps. 78 FR 12252. The document provided for...

7 CFR 1469.25 - Contract violations and termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... termination without delay. (c) If NRCS terminates a contract due to breach of contract, the participant will... terminates a contract due to breach of contract, or the participant voluntarily terminates the contract... 7 Agriculture 10 2010-01-01 2010-01-01 false Contract violations and termination. 1469.25 Section...

29 CFR 4041.42 - Administration of plan during termination process.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 9 2010-07-01 2010-07-01 false Administration of plan during termination process. 4041.42... TERMINATIONS TERMINATION OF SINGLE-EMPLOYER PLANS Distress Termination Process § 4041.42 Administration of plan during termination process. (a) General rule. Except to the extent specifically prohibited by this...

Trigger loop dynamics can explain stimulation of intrinsic termination by bacterial RNA polymerase without terminator hairpin contact.

PubMed

Ray-Soni, Ananya; Mooney, Rachel A; Landick, Robert

2017-10-31

In bacteria, intrinsic termination signals cause disassembly of the highly stable elongating transcription complex (EC) over windows of two to three nucleotides after kilobases of RNA synthesis. Intrinsic termination is caused by the formation of a nascent RNA hairpin adjacent to a weak RNA-DNA hybrid within RNA polymerase (RNAP). Although the contributions of RNA and DNA sequences to termination are largely understood, the roles of conformational changes in RNAP are less well described. The polymorphous trigger loop (TL), which folds into the trigger helices to promote nucleotide addition, also is proposed to drive termination by folding into the trigger helices and contacting the terminator hairpin after invasion of the hairpin in the RNAP main cleft [Epshtein V, Cardinale CJ, Ruckenstein AE, Borukhov S, Nudler E (2007) Mol Cell 28:991-1001]. To investigate the contribution of the TL to intrinsic termination, we developed a kinetic assay that distinguishes effects of TL alterations on the rate at which ECs terminate from effects of the TL on the nucleotide addition rate that indirectly affect termination efficiency by altering the time window in which termination can occur. We confirmed that the TL stimulates termination rate, but found that stabilizing either the folded or unfolded TL conformation decreased termination rate. We propose that conformational fluctuations of the TL (TL dynamics), not TL-hairpin contact, aid termination by increasing EC conformational diversity and thus access to favorable termination pathways. We also report that the TL and the TL sequence insertion (SI3) increase overall termination efficiency by stimulating pausing, which increases the flux of ECs into the termination pathway. Published under the PNAS license.

Trigger loop dynamics can explain stimulation of intrinsic termination by bacterial RNA polymerase without terminator hairpin contact

PubMed Central

Ray-Soni, Ananya; Mooney, Rachel A.; Landick, Robert

2017-01-01

In bacteria, intrinsic termination signals cause disassembly of the highly stable elongating transcription complex (EC) over windows of two to three nucleotides after kilobases of RNA synthesis. Intrinsic termination is caused by the formation of a nascent RNA hairpin adjacent to a weak RNA−DNA hybrid within RNA polymerase (RNAP). Although the contributions of RNA and DNA sequences to termination are largely understood, the roles of conformational changes in RNAP are less well described. The polymorphous trigger loop (TL), which folds into the trigger helices to promote nucleotide addition, also is proposed to drive termination by folding into the trigger helices and contacting the terminator hairpin after invasion of the hairpin in the RNAP main cleft [Epshtein V, Cardinale CJ, Ruckenstein AE, Borukhov S, Nudler E (2007) Mol Cell 28:991–1001]. To investigate the contribution of the TL to intrinsic termination, we developed a kinetic assay that distinguishes effects of TL alterations on the rate at which ECs terminate from effects of the TL on the nucleotide addition rate that indirectly affect termination efficiency by altering the time window in which termination can occur. We confirmed that the TL stimulates termination rate, but found that stabilizing either the folded or unfolded TL conformation decreased termination rate. We propose that conformational fluctuations of the TL (TL dynamics), not TL-hairpin contact, aid termination by increasing EC conformational diversity and thus access to favorable termination pathways. We also report that the TL and the TL sequence insertion (SI3) increase overall termination efficiency by stimulating pausing, which increases the flux of ECs into the termination pathway. PMID:29078293

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

PubMed Central

Liskova, Jana; Babchenko, Oleg; Varga, Marian; Kromka, Alexander; Hadraba, Daniel; Svindrych, Zdenek; Burdikova, Zuzana; Bacakova, Lucie

2015-01-01

Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings. PMID:25670900

Does Individual Gambling Behavior Vary across Gambling Venues with Differing Numbers of Terminals? An Empirical Real-World Study using Player Account Data.

PubMed

Sagoe, Dominic; Pallesen, Ståle; Griffiths, Mark D; Mentzoni, Rune A; Leino, Tony

2018-01-01

Research examining gambling behavior via experiments, self-report, and/or observation presents many methodical challenges particularly in relation to objectivity. However, the use of player account-based gambling data provides purely objective data. Based on this real-world data, the primary aim of the present study was to examine gambling behavior in gambling venues with different numbers of gambling terminals (i.e., venues with one terminal; 2-5 terminals; 6-10 terminals; 11-16 terminals). Player account-based gambling data aggregated over a year (2015) amounting to 153,379 observations within 93,034 individual gamblers (males = 74%; mean age = 44.1, SD = 16.4 years) were analyzed. Gambling frequency was highest in venues with 2-5 terminals (54.5%) and lowest in venues with 11-16 terminals (1.6%). Approximately half of the sample (52.5%) gambled in only one venue category, with the majority (81.5%) preferring venues with 2-5 terminals present. Only 0.8% of the sample gambled in all four venue categories. Compared to venues with one terminal, venues with two or more terminals were associated with gamblers placing more bets, and spending more time and money per session. However, gamblers had higher losses (albeit small) in venues with one terminal compared to venues with 2-5 terminals. No differences in net outcome were found between venues with one terminal and those with 6-10 and 11-16 terminals. Overall, the present study demonstrates that in the natural gambling environment, gambling behavior is reinforced in venues with multiple terminals.

Problem-Solving Test: The Mechanism of Transcription Termination by the Rho Factor

ERIC Educational Resources Information Center

Szeberenyi, Jozsef

2012-01-01

Transcription termination comes in two forms in "E. coli" cells. Rho-dependent termination requires the binding of a termination protein called Rho factor to the transcriptional machinery at the terminator region, whereas Rho-independent termination is achieved by conformational changes in the transcript itself. This article presents a test…

Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator.

PubMed

Hollands, Kerry; Sevostiyanova, Anastasia; Groisman, Eduardo A

2014-05-13

Up to half of all transcription termination events in bacteria rely on the RNA-dependent helicase Rho. However, the nucleic acid sequences that promote Rho-dependent termination remain poorly characterized. Defining the molecular determinants that confer Rho-dependent termination is especially important for understanding how such terminators can be regulated in response to specific signals. Here, we identify an extraordinarily long-lived pause at the site where Rho terminates transcription in the 5'-leader region of the Mg(2+) transporter gene mgtA in Salmonella enterica. We dissect the sequence elements required for prolonged pausing in the mgtA leader and establish that the remarkable longevity of this pause is required for a riboswitch to stimulate Rho-dependent termination in the mgtA leader region in response to Mg(2+) availability. Unlike Rho-dependent terminators described previously, where termination occurs at multiple pause sites, there is a single site of transcription termination directed by Rho in the mgtA leader. Our data suggest that Rho-dependent termination events that are subject to regulation may require elements distinct from those operating at constitutive Rho-dependent terminators.

Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator.

PubMed

Li, Rui; Zhang, Qing; Li, Junbai; Shi, Hualin

2016-04-07

An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position-function relations were quantified for the terminator tR2 in Escherichia coli The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE-distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Does Individual Gambling Behavior Vary across Gambling Venues with Differing Numbers of Terminals? An Empirical Real-World Study using Player Account Data

PubMed Central

Sagoe, Dominic; Pallesen, Ståle; Griffiths, Mark D.; Mentzoni, Rune A.; Leino, Tony

2018-01-01

Research examining gambling behavior via experiments, self-report, and/or observation presents many methodical challenges particularly in relation to objectivity. However, the use of player account-based gambling data provides purely objective data. Based on this real-world data, the primary aim of the present study was to examine gambling behavior in gambling venues with different numbers of gambling terminals (i.e., venues with one terminal; 2–5 terminals; 6–10 terminals; 11–16 terminals). Player account-based gambling data aggregated over a year (2015) amounting to 153,379 observations within 93,034 individual gamblers (males = 74%; mean age = 44.1, SD = 16.4 years) were analyzed. Gambling frequency was highest in venues with 2–5 terminals (54.5%) and lowest in venues with 11–16 terminals (1.6%). Approximately half of the sample (52.5%) gambled in only one venue category, with the majority (81.5%) preferring venues with 2–5 terminals present. Only 0.8% of the sample gambled in all four venue categories. Compared to venues with one terminal, venues with two or more terminals were associated with gamblers placing more bets, and spending more time and money per session. However, gamblers had higher losses (albeit small) in venues with one terminal compared to venues with 2–5 terminals. No differences in net outcome were found between venues with one terminal and those with 6–10 and 11–16 terminals. Overall, the present study demonstrates that in the natural gambling environment, gambling behavior is reinforced in venues with multiple terminals. PMID:29503626

Definition of RNA Polymerase II CoTC Terminator Elements in the Human Genome

PubMed Central

Nojima, Takayuki; Dienstbier, Martin; Murphy, Shona; Proudfoot, Nicholas J.; Dye, Michael J.

2013-01-01

Summary Mammalian RNA polymerase II (Pol II) transcription termination is an essential step in protein-coding gene expression that is mediated by pre-mRNA processing activities and DNA-encoded terminator elements. Although much is known about the role of pre-mRNA processing in termination, our understanding of the characteristics and generality of terminator elements is limited. Whereas promoter databases list up to 40,000 known and potential Pol II promoter sequences, fewer than ten Pol II terminator sequences have been described. Using our knowledge of the human β-globin terminator mechanism, we have developed a selection strategy for mapping mammalian Pol II terminator elements. We report the identification of 78 cotranscriptional cleavage (CoTC)-type terminator elements at endogenous gene loci. The results of this analysis pave the way for the full understanding of Pol II termination pathways and their roles in gene expression. PMID:23562152

Evolvable circuit with transistor-level reconfigurability

NASA Technical Reports Server (NTRS)

Stoica, Adrian (Inventor); Salazar-Lazaro, Carlos Harold (Inventor)

2004-01-01

An evolvable circuit includes a plurality of reconfigurable switches, a plurality of transistors within a region of the circuit, the plurality of transistors having terminals, the plurality of transistors being coupled between a power source terminal and a power sink terminal so as to be capable of admitting power between the power source terminal and the power sink terminal, the plurality of transistors being coupled so that every transistor terminal to transistor terminal coupling within the region of the circuit comprises a reconfigurable switch.

AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination

PubMed Central

White, Eleanor; Kamieniarz-Gdula, Kinga; Dye, Michael J.; Proudfoot, Nick J.

2013-01-01

RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tracts within the human β-globin CoTC-mediated terminator element play a critical role in Pol II termination. These short A/T tracts, dispersed within seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirms the presence of such sequences in 70% of the putative terminator regions (PTRs) genome-wide. PMID:23258704

Dynamic probability of reinforcement for cooperation: Random game termination in the centipede game.

PubMed

Krockow, Eva M; Colman, Andrew M; Pulford, Briony D

2018-03-01

Experimental games have previously been used to study principles of human interaction. Many such games are characterized by iterated or repeated designs that model dynamic relationships, including reciprocal cooperation. To enable the study of infinite game repetitions and to avoid endgame effects of lower cooperation toward the final game round, investigators have introduced random termination rules. This study extends previous research that has focused narrowly on repeated Prisoner's Dilemma games by conducting a controlled experiment of two-player, random termination Centipede games involving probabilistic reinforcement and characterized by the longest decision sequences reported in the empirical literature to date (24 decision nodes). Specifically, we assessed mean exit points and cooperation rates, and compared the effects of four different termination rules: no random game termination, random game termination with constant termination probability, random game termination with increasing termination probability, and random game termination with decreasing termination probability. We found that although mean exit points were lower for games with shorter expected game lengths, the subjects' cooperativeness was significantly reduced only in the most extreme condition with decreasing computer termination probability and an expected game length of two decision nodes. © 2018 Society for the Experimental Analysis of Behavior.

Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator

PubMed Central

Hollands, Kerry; Sevostiyanova, Anastasia; Groisman, Eduardo A.

2014-01-01

Up to half of all transcription termination events in bacteria rely on the RNA-dependent helicase Rho. However, the nucleic acid sequences that promote Rho-dependent termination remain poorly characterized. Defining the molecular determinants that confer Rho-dependent termination is especially important for understanding how such terminators can be regulated in response to specific signals. Here, we identify an extraordinarily long-lived pause at the site where Rho terminates transcription in the 5′-leader region of the Mg2+ transporter gene mgtA in Salmonella enterica. We dissect the sequence elements required for prolonged pausing in the mgtA leader and establish that the remarkable longevity of this pause is required for a riboswitch to stimulate Rho-dependent termination in the mgtA leader region in response to Mg2+ availability. Unlike Rho-dependent terminators described previously, where termination occurs at multiple pause sites, there is a single site of transcription termination directed by Rho in the mgtA leader. Our data suggest that Rho-dependent termination events that are subject to regulation may require elements distinct from those operating at constitutive Rho-dependent terminators. PMID:24778260

Confocal Laser Scanning Microscopy and Ultrastructural Study of VGLUT2 Thalamic Input to Striatal Projection Neurons in Rats

PubMed Central

Lei, Wanlong; Deng, Yunping; Liu, Bingbing; Mu, Shuhua; Guley, Natalie M.; Wong, Ting; Reiner, Anton

2014-01-01

We examined thalamic input to striatum in rats using immunolabeling for the vesicular glutamate transporter (VGLUT2). Double immunofluorescence viewed with confocal laser scanning microscopy (CLSM) revealed that VGLUT2+ terminals are distinct from VGLUT1+ terminals. CLSM of Phaseolus vulgaris-leucoagglutinin (PHAL)-labeled cortical or thalamic terminals revealed that VGLUT2 is rare in corticostriatal terminals but nearly always present in thalamostriatal terminals. Electron microscopy revealed that VGLUT2+ terminals made up 39.4% of excitatory terminals in striatum (with VGLUT1+ corticostriatal terminals constituting the rest), and 66.8% of VGLUT2+ terminals synapsed on spines and the remainder on dendrites. VGLUT2+ axo-spinous terminals had a mean diameter of 0.624 lm, while VGLUT2+ axodendritic terminals a mean diameter of 0.698 µm. In tissue in which we simultaneously immunolabeled thalamostriatal terminals for VGLUT2 and striatal neurons for D1 (with about half of spines immunolabeled for D1), 54.6% of VGLUT2+ terminals targeted D1+ spines (i.e., direct pathway striatal neurons), and 37.3% of D1+ spines received VGLUT2+ synaptic contacts. By contrast, 45.4% of VGLUT2+ terminals targeted D1-negative spines (i.e., indirect pathway striatal neurons), and only 25.8% of D1-negative spines received VGLUT2+ synaptic contacts. Similarly, among VGLUT2+ axodendritic synaptic terminals, 59.1% contacted D1+ dendrites, and 40.9% contacted D1-negative dendrites. VGLUT2+ terminals on D1+ spines and dendrites tended to be slightly smaller than those on D1-negative spines and dendrites. Thus, thala-mostriatal terminals contact both direct and indirect pathway striatal neurons, with a slight preference for direct. These results are consistent with physiological studies indicating slightly different effects of thalamic input on the two types of striatal projection neurons. PMID:23047588

Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats.

PubMed

Lei, Wanlong; Deng, Yunping; Liu, Bingbing; Mu, Shuhua; Guley, Natalie M; Wong, Ting; Reiner, Anton

2013-04-15

We examined thalamic input to striatum in rats using immunolabeling for the vesicular glutamate transporter (VGLUT2). Double immunofluorescence viewed with confocal laser scanning microscopy (CLSM) revealed that VGLUT2+ terminals are distinct from VGLUT1+ terminals. CLSM of Phaseolus vulgaris-leucoagglutinin (PHAL)-labeled cortical or thalamic terminals revealed that VGLUT2 is rare in corticostriatal terminals but nearly always present in thalamostriatal terminals. Electron microscopy revealed that VGLUT2+ terminals made up 39.4% of excitatory terminals in striatum (with VGLUT1+ corticostriatal terminals constituting the rest), and 66.8% of VGLUT2+ terminals synapsed on spines and the remainder on dendrites. VGLUT2+ axospinous terminals had a mean diameter of 0.624 μm, while VGLUT2+ axodendritic terminals a mean diameter of 0.698 μm. In tissue in which we simultaneously immunolabeled thalamostriatal terminals for VGLUT2 and striatal neurons for D1 (with about half of spines immunolabeled for D1), 54.6% of VGLUT2+ terminals targeted D1+ spines (i.e., direct pathway striatal neurons), and 37.3% of D1+ spines received VGLUT2+ synaptic contacts. By contrast, 45.4% of VGLUT2+ terminals targeted D1-negative spines (i.e., indirect pathway striatal neurons), and only 25.8% of D1-negative spines received VGLUT2+ synaptic contacts. Similarly, among VGLUT2+ axodendritic synaptic terminals, 59.1% contacted D1+ dendrites, and 40.9% contacted D1-negative dendrites. VGLUT2+ terminals on D1+ spines and dendrites tended to be slightly smaller than those on D1-negative spines and dendrites. Thus, thalamostriatal terminals contact both direct and indirect pathway striatal neurons, with a slight preference for direct. These results are consistent with physiological studies indicating slightly different effects of thalamic input on the two types of striatal projection neurons. Copyright © 2012 Wiley Periodicals, Inc.

The dimerization of half-molecule fragments of transferrin.

PubMed Central

Williams, J; Moreton, K

1988-01-01

Partial proteolysis was used to prepare half-molecule fragments of hen ovotransferrin. N-Terminal and C-terminal fragments associate to form an N-terminal fragment-C-terminal fragment dimer. Variant forms of the N- and C-terminal fragments can be prepared in which a few amino acid residues are lacking from the C-terminal ends of the fragments. These variant fragments are partially or completely unable to associate; the suggestion that the molecular recognition sites are located in these C-terminal stretches of the N-terminal half-molecule (320-332) and of the C-terminal half-molecule (683-686) is in agreement with X-ray-crystallography data for human lactotransferrin [Anderson, Baker, Dodson, Norris, Rumball, Waters & Baker (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 1769-1773]. PMID:3415649

Low inductance busbar assembly

DOEpatents

Holbrook, Meghan Ann

2010-09-21

A busbar assembly for electrically coupling first and second busbars to first and second contacts, respectively, on a power module is provided. The assembly comprises a first terminal integrally formed with the first busbar, a second terminal integrally formed with the second busbar and overlapping the first terminal, a first bridge electrode having a first tab electrically coupled to the first terminal and overlapping the first and second terminals, and a second tab electrically coupled to the first contact, a second bridge electrode having a third tab electrically coupled to the second terminal, and overlapping the first and second terminals and the first tab, and a fourth tab electrically coupled to the second contact, and a fastener configured to couple the first tab to the first terminal, and the third tab to the second terminal.

The NH2-terminal and COOH-terminal fragments of dentin matrix protein 1 (DMP1) localize differently in the compartments of dentin and growth plate of bone.

PubMed

Maciejewska, Izabela; Cowan, Cameron; Svoboda, Kathy; Butler, William T; D'Souza, Rena; Qin, Chunlin

2009-02-01

Multiple studies have shown that dentin matrix protein 1 (DMP1) is essential for bone and dentin mineralization. After post-translational proteolytic cleavage, DMP1 exists within the extracellular matrix of bone and dentin as an NH2-terminal fragment, a COOH-terminal fragment, and the proteoglycan form of the NH2-terminal fragment (DMP1-PG). To begin to assess the biological function of each fragment, we evaluated the distribution of both fragments in the rat tooth and bone using antibodies specific to the NH2-terminal and COOH-terminal regions of DMP1 and confocal microscopy. In rat first molar organs, the NH2-terminal fragment localized to predentin, whereas the COOH-terminal fragment was mainly restricted to mineralized dentin. In the growth plate of bone, the NH2-terminal fragment appeared in the proliferation and hypertrophic zones, whereas the COOH-terminal fragment occupied the ossification zone. Forster resonance energy transfer analysis showed colocalization of both fragments of DMP1 in odontoblasts and predentin, as well as hypertrophic chondrocytes within the growth plates of bone. The biochemical analysis of bovine teeth showed that predentin is rich in DMP1-PG, whereas mineralized dentin primarily contains the COOH-terminal fragment. We conclude that the differential patterns of expression of NH2-terminal and COOH-terminal fragments of DMP1 reflect their potentially distinct roles in the biomineralization of dentin and bone matrices.