33 CFR 117.263 - Banana River.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Banana River. 117.263 Section 117.263 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.263 Banana River. (a) The draw of the Mathers (SR...

33 CFR 117.263 - Banana River.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Banana River. 117.263 Section 117.263 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.263 Banana River. (a) The draw of the Mathers (SR...

33 CFR 117.263 - Banana River.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Banana River. 117.263 Section 117.263 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.263 Banana River. (a) The draw of the Mathers (SR...

33 CFR 117.263 - Banana River.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Banana River. 117.263 Section 117.263 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.263 Banana River. (a) The draw of the Mathers (SR...

12 CFR 263.29 - Summary disposition.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Summary disposition. 263.29 Section 263.29... RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.29 Summary disposition. (a... a motion for summary disposition if the undisputed pleaded facts, admissions, affidavits...

32 CFR 263.3 - Compliance.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Compliance. 263.3 Section 263.3 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.3 Compliance. (a) All persons entering the...

32 CFR 263.3 - Compliance.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Compliance. 263.3 Section 263.3 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.3 Compliance. (a) All persons entering the...

12 CFR 263.99 - Petition for reinstatement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Petition for reinstatement. 263.99 Section 263... SYSTEM RULES OF PRACTICE FOR HEARINGS Practice Before the Board § 263.99 Petition for reinstatement. The Board may entertain a petition for reinstatement from any person debarred from practice before the Board...

32 CFR 263.4 - Registration of vehicles.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false Registration of vehicles. 263.4 Section 263.4...) MISCELLANEOUS TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.4 Registration of vehicles. (a) Newly assigned or employed individuals who intend to operate a privately-owned vehicle at the...

32 CFR 263.4 - Registration of vehicles.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Registration of vehicles. 263.4 Section 263.4...) MISCELLANEOUS TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.4 Registration of vehicles. (a) Newly assigned or employed individuals who intend to operate a privately-owned vehicle at the...

32 CFR 263.4 - Registration of vehicles.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Registration of vehicles. 263.4 Section 263.4...) MISCELLANEOUS TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.4 Registration of vehicles. (a) Newly assigned or employed individuals who intend to operate a privately-owned vehicle at the...

27 CFR 25.263 - Production of concentrate and reconstitution of beer.

Code of Federal Regulations, 2010 CFR

2010-04-01

... and reconstitution of beer. 25.263 Section 25.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Beer Concentrate § 25.263 Production of concentrate and reconstitution of beer. (a) Operations at brewery. A brewer may concentrate beer...

27 CFR 25.263 - Production of concentrate and reconstitution of beer.

Code of Federal Regulations, 2013 CFR

2013-04-01

... and reconstitution of beer. 25.263 Section 25.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL BEER Beer Concentrate § 25.263 Production of concentrate and reconstitution of beer. (a) Operations at brewery. A brewer may concentrate beer...

27 CFR 25.263 - Production of concentrate and reconstitution of beer.

Code of Federal Regulations, 2012 CFR

2012-04-01

... and reconstitution of beer. 25.263 Section 25.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Beer Concentrate § 25.263 Production of concentrate and reconstitution of beer. (a) Operations at brewery. A brewer may concentrate beer...

27 CFR 25.263 - Production of concentrate and reconstitution of beer.

Code of Federal Regulations, 2014 CFR

2014-04-01

... and reconstitution of beer. 25.263 Section 25.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL BEER Beer Concentrate § 25.263 Production of concentrate and reconstitution of beer. (a) Operations at brewery. A brewer may concentrate beer...

40 CFR 60.263 - Standard for carbon monoxide.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Standard for carbon monoxide. 60.263 Section 60.263 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Ferroalloy Production Facilities § 60.263 Standard for carbo...

40 CFR 60.263 - Standard for carbon monoxide.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Standard for carbon monoxide. 60.263 Section 60.263 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Ferroalloy Production Facilities § 60.263 Standard for carbo...

40 CFR 60.263 - Standard for carbon monoxide.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Standard for carbon monoxide. 60.263 Section 60.263 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Ferroalloy Production Facilities § 60.263 Standard for carbo...

39 CFR 263.2 - Policy.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 39 Postal Service 1 2011-07-01 2011-07-01 false Policy. 263.2 Section 263.2 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION RECORDS RETENTION AND DISPOSITION § 263.2 Policy. It is the policy of the U.S. Postal Service to establish and maintain schedules specifying the...

39 CFR 263.2 - Policy.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 39 Postal Service 1 2014-07-01 2014-07-01 false Policy. 263.2 Section 263.2 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION RECORDS RETENTION AND DISPOSITION § 263.2 Policy. It is the policy of the U.S. Postal Service to establish and maintain schedules specifying the...

12 CFR 263.405 - Petition for reinstatement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Petition for reinstatement. 263.405 Section 263.405 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE... Audit Services § 263.405 Petition for reinstatement. (a) Form of petition. Unless otherwise ordered by...

27 CFR 25.263 - Production of concentrate and reconstitution of beer.

Code of Federal Regulations, 2011 CFR

2011-04-01

... and reconstitution of beer. 25.263 Section 25.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Beer Concentrate § 25.263 Production of concentrate and reconstitution of beer. (a) Operations at brewery. A brewer may concentrate beer...

34 CFR 263.9 - When does payback begin?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false When does payback begin? 263.9 Section 263.9 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION INDIAN EDUCATION DISCRETIONARY GRANT PROGRAMS Professional Development Program § 263.9 When does payback begin? (a) For...

12 CFR 263.7 - Good faith certification.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Good faith certification. 263.7 Section 263.7... (CONTINUED) RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.7 Good faith... in fact and is warranted by existing law or a good faith argument for the extension, modification, or...

12 CFR 263.7 - Good faith certification.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Good faith certification. 263.7 Section 263.7... RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.7 Good faith certification... in fact and is warranted by existing law or a good faith argument for the extension, modification, or...

12 CFR 263.7 - Good faith certification.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Good faith certification. 263.7 Section 263.7... (CONTINUED) RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.7 Good faith... in fact and is warranted by existing law or a good faith argument for the extension, modification, or...

12 CFR 263.7 - Good faith certification.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Good faith certification. 263.7 Section 263.7... (CONTINUED) RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.7 Good faith... in fact and is warranted by existing law or a good faith argument for the extension, modification, or...

12 CFR 263.7 - Good faith certification.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Good faith certification. 263.7 Section 263.7... RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.7 Good faith certification... in fact and is warranted by existing law or a good faith argument for the extension, modification, or...

26 CFR 1.263A-12 - Production period.

Code of Federal Regulations, 2013 CFR

2013-04-01

... required to capitalize the $100,000 interest on traced debt incurred during the two measurement periods... 26 Internal Revenue 3 2013-04-01 2013-04-01 false Production period. 1.263A-12 Section 1.263A-12...) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-12 Production period. (a) In general...

26 CFR 1.263A-12 - Production period.

Code of Federal Regulations, 2012 CFR

2012-04-01

... required to capitalize the $100,000 interest on traced debt incurred during the two measurement periods... 26 Internal Revenue 3 2012-04-01 2012-04-01 false Production period. 1.263A-12 Section 1.263A-12...) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-12 Production period. (a) In general...

26 CFR 1.263A-12 - Production period.

Code of Federal Regulations, 2014 CFR

2014-04-01

... required to capitalize the $100,000 interest on traced debt incurred during the two measurement periods... 26 Internal Revenue 3 2014-04-01 2014-04-01 false Production period. 1.263A-12 Section 1.263A-12...) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-12 Production period. (a) In general...

26 CFR 1.263A-12 - Production period.

Code of Federal Regulations, 2011 CFR

2011-04-01

... required to capitalize the $100,000 interest on traced debt incurred during the two measurement periods... 26 Internal Revenue 3 2011-04-01 2011-04-01 false Production period. 1.263A-12 Section 1.263A-12...) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-12 Production period. (a) In general...

32 CFR 263.7 - Emergency vehicles.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 2 2013-07-01 2013-07-01 false Emergency vehicles. 263.7 Section 263.7 National... TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.7 Emergency vehicles. No person shall fail or refuse to yield the right-of-way to an emergency vehicle when operating with siren or...

32 CFR 263.7 - Emergency vehicles.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false Emergency vehicles. 263.7 Section 263.7 National... TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.7 Emergency vehicles. No person shall fail or refuse to yield the right-of-way to an emergency vehicle when operating with siren or...

32 CFR 263.7 - Emergency vehicles.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Emergency vehicles. 263.7 Section 263.7 National... TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.7 Emergency vehicles. No person shall fail or refuse to yield the right-of-way to an emergency vehicle when operating with siren or...

32 CFR 263.7 - Emergency vehicles.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Emergency vehicles. 263.7 Section 263.7 National... TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.7 Emergency vehicles. No person shall fail or refuse to yield the right-of-way to an emergency vehicle when operating with siren or...

32 CFR 263.7 - Emergency vehicles.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Emergency vehicles. 263.7 Section 263.7 National... TRAFFIC AND VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.7 Emergency vehicles. No person shall fail or refuse to yield the right-of-way to an emergency vehicle when operating with siren or...

12 CFR 263.12 - Construction of time limits.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Construction of time limits. 263.12 Section 263.12 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.12 Construction of...

28 CFR 26.3 - Date, time, place, and method of execution.

Code of Federal Regulations, 2010 CFR

2010-07-01

... intravenous injection of a lethal substance or substances in a quantity sufficient to cause death, such... execution. 26.3 Section 26.3 Judicial Administration DEPARTMENT OF JUSTICE DEATH SENTENCES PROCEDURES Implementation of Death Sentences in Federal Cases § 26.3 Date, time, place, and method of execution. (a) Except...

12 CFR 263.8 - Conflicts of interest.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Conflicts of interest. 263.8 Section 263.8... RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.8 Conflicts of interest. (a) Conflict of interest in representation. No person shall appear as counsel for another person in an...

10 CFR 26.3 - Scope.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 1 2014-01-01 2014-01-01 false Scope. 26.3 Section 26.3 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Administrative Provisions § 26.3 Scope. (a) Licensees who are authorized to operate a nuclear power reactor under 10 CFR 50.57, and holders of a combined license under 10 CFR...

10 CFR 26.3 - Scope.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 1 2013-01-01 2013-01-01 false Scope. 26.3 Section 26.3 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Administrative Provisions § 26.3 Scope. (a) Licensees who are authorized to operate a nuclear power reactor under 10 CFR 50.57, and holders of a combined license under 10 CFR...

10 CFR 26.3 - Scope.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 1 2012-01-01 2012-01-01 false Scope. 26.3 Section 26.3 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Administrative Provisions § 26.3 Scope. (a) Licensees who are authorized to operate a nuclear power reactor under 10 CFR 50.57, and holders of a combined license under 10 CFR...

10 CFR 26.3 - Scope.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Scope. 26.3 Section 26.3 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Administrative Provisions § 26.3 Scope. (a) Licensees who are authorized to operate a nuclear power reactor under 10 CFR 50.57, and holders of a combined license under 10 CFR...

10 CFR 26.3 - Scope.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Scope. 26.3 Section 26.3 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Administrative Provisions § 26.3 Scope. (a) Licensees who are authorized to operate a nuclear power reactor under 10 CFR 50.57, and holders of a combined license under 10 CFR...

12 CFR 263.54 - Delegation to the Office of Financial Institution Adjudication.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Uniform Rules § 263.54 Delegation to the Office of Financial Institution Adjudication. Unless otherwise... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Delegation to the Office of Financial Institution Adjudication. 263.54 Section 263.54 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF...

12 CFR 263.11 - Service of papers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Service of papers. 263.11 Section 263.11 Banks... OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.11 Service of papers. (a) By the parties. Except as otherwise provided, a party filing papers shall serve a copy upon the counsel...

12 CFR 263.10 - Filing of papers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Filing of papers. 263.10 Section 263.10 Banks... OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.10 Filing of papers. (a) Filing. Any papers required to be filed, excluding documents produced in response to a discovery request...

12 CFR 263.10 - Filing of papers.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Filing of papers. 263.10 Section 263.10 Banks... OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.10 Filing of papers. (a) Filing. Any papers required to be filed, excluding documents produced in response to a discovery request...

12 CFR 263.11 - Service of papers.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Service of papers. 263.11 Section 263.11 Banks... OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.11 Service of papers. (a) By the parties. Except as otherwise provided, a party filing papers shall serve a copy upon the counsel...

12 CFR 263.21 - Failure to appear.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Failure to appear. 263.21 Section 263.21 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.21 Failure to appear. Failure...

12 CFR 263.30 - Partial summary disposition.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Partial summary disposition. 263.30 Section 263... SYSTEM RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.30 Partial summary disposition. If the administrative law judge determines that a party is entitled to summary disposition as to...

FURTHER OBSERVATIONS ON THE SIGNIFICANCE OF A/EQUINE-2/63 ANTIBODIES IN MAN

PubMed Central

Davenport, F. M.; Hennessy, A. V.; Minuse, Elva

1967-01-01

The antibody pattern of equine-2/63 viruses has been more sharply defined using a large number of human sera collected in 1964. The birth dates of persons exhibiting the richest experience with equine-2/63-like viruses delineate a period of past prevalence in man of equine-2/63-like viruses. The period is believed to have begun in the mid-1870's and to have terminated in 1889–1890 at the time of the first Asian pandemic. The equine-2/63 antibodies found in human sera react specifically in the photometric test of Drescher. The equine-2/63 antibody pattern advances along the age scale in exact concordance with the passage of time. The homologous antibody response of the older subjects to equine-2/63 vaccine is more vigorous, reflecting the conditioning effects of prior exposures to equine-2/63 antigens. A "one-way cross" between equine-2/63 virus and A2 and A1 strains has been demonstrated. The antigenic ties between strains of influenza A isolated from humans, swine, horses, and birds is recognized and discussed. It is apparent that horses do not constitute an active reservoir for strains of human involvement. The epidemiologic significance of the antigenic linkages between strains isolated from different species remains obscure. PMID:6069928

Mechanism of artemisinin resistance for malaria PfATP6 L263 mutations and discovering potential antimalarials: An integrated computational approach

NASA Astrophysics Data System (ADS)

Nagasundaram, N.; George Priya Doss, C.; Chakraborty, Chiranjib; Karthick, V.; Thirumal Kumar, D.; Balaji, V.; Siva, R.; Lu, Aiping; Ge, Zhang; Zhu, Hailong

2016-07-01

Artemisinin resistance in Plasmodium falciparum threatens global efforts in the elimination or eradication of malaria. Several studies have associated mutations in the PfATP6 gene in conjunction with artemisinin resistance, but the underlying molecular mechanism of the resistance remains unexplored. Associated mutations act as a biomarker to measure the artemisinin efficacy. In the proposed work, we have analyzed the binding affinity and efficacy between PfATP6 and artemisinin in the presence of L263D, L263E and L263K mutations. Furthermore, we performed virtual screening to identify potential compounds to inhibit the PfATP6 mutant proteins. In this study, we observed that artemisinin binding affinity with PfATP6 gets affected by L263D, L263E and L263K mutations. This in silico elucidation of artemisinin resistance enhanced the identification of novel compounds (CID: 10595058 and 10625452) which showed good binding affinity and efficacy with L263D, L263E and L263K mutant proteins in molecular docking and molecular dynamics simulations studies. Owing to the high propensity of the parasite to drug resistance the need for new antimalarial drugs will persist until the malarial parasites are eventually eradicated. The two compounds identified in this study can be tested in in vitro and in vivo experiments as possible candidates for the designing of new potential antimalarial drugs.

33 CFR 263.24 - Authority for snagging and clearing for flood control (Section 208).

Code of Federal Regulations, 2010 CFR

2010-07-01

... clearing for flood control (Section 208). 263.24 Section 263.24 Navigation and Navigable Waters CORPS OF... Policy § 263.24 Authority for snagging and clearing for flood control (Section 208). (a) Legislative... 26 of the Water Resources Development Act approved March 7, 1974 states: The Secretary of the Army is...

7 CFR 2.63 - Deputy Under Secretary for Research, Education, and Economics.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Economics. 2.63 Section 2.63 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Under Secretary for Research, Education, and Economics § 2.63 Deputy Under Secretary for Research, Education, and Economics. Pursuant to § 2.21(a), subject to reservations in § 2.21(b), and subject to policy...

7 CFR 2.63 - Deputy Under Secretary for Research, Education, and Economics.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Economics. 2.63 Section 2.63 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Under Secretary for Research, Education, and Economics § 2.63 Deputy Under Secretary for Research, Education, and Economics. Pursuant to § 2.21(a), subject to reservations in § 2.21(b), and subject to policy...

7 CFR 2.63 - Deputy Under Secretary for Research, Education, and Economics.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Economics. 2.63 Section 2.63 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Under Secretary for Research, Education, and Economics § 2.63 Deputy Under Secretary for Research, Education, and Economics. Pursuant to § 2.21(a), subject to reservations in § 2.21(b), and subject to policy...

7 CFR 2.63 - Deputy Under Secretary for Research, Education, and Economics.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Economics. 2.63 Section 2.63 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Under Secretary for Research, Education, and Economics § 2.63 Deputy Under Secretary for Research, Education, and Economics. Pursuant to § 2.21(a), subject to reservations in § 2.21(b), and subject to policy...

7 CFR 2.63 - Deputy Under Secretary for Research, Education, and Economics.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Economics. 2.63 Section 2.63 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Under Secretary for Research, Education, and Economics § 2.63 Deputy Under Secretary for Research, Education, and Economics. Pursuant to § 2.21(a), subject to reservations in § 2.21(b), and subject to policy...

34 CFR 263.2 - Who is eligible to apply under the Professional Development program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... GRANT PROGRAMS Professional Development Program § 263.2 Who is eligible to apply under the Professional... 34 Education 1 2010-07-01 2010-07-01 false Who is eligible to apply under the Professional Development program? 263.2 Section 263.2 Education Regulations of the Offices of the Department of Education...

34 CFR 263.2 - Who is eligible to apply under the Professional Development program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... GRANT PROGRAMS Professional Development Program § 263.2 Who is eligible to apply under the Professional... 34 Education 1 2011-07-01 2011-07-01 false Who is eligible to apply under the Professional Development program? 263.2 Section 263.2 Education Regulations of the Offices of the Department of Education...

12 CFR 263.21 - Failure to appear.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Failure to appear. 263.21 Section 263.21 Banks... (CONTINUED) RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.21 Failure to appear. Failure of a respondent to appear in person at the hearing or by a duly authorized counsel constitutes a...

12 CFR 263.21 - Failure to appear.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Failure to appear. 263.21 Section 263.21 Banks... (CONTINUED) RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.21 Failure to appear. Failure of a respondent to appear in person at the hearing or by a duly authorized counsel constitutes a...

45 CFR 263.9 - May a State avoid a penalty for failing to meet the basic MOE requirement through reasonable...

Code of Federal Regulations, 2010 CFR

2010-10-01

... the basic MOE requirement through reasonable cause or corrective compliance? 263.9 Section 263.9... FEDERAL TANF FUNDS What Rules Apply to a State's Maintenance of Effort? § 263.9 May a State avoid a penalty for failing to meet the basic MOE requirement through reasonable cause or corrective compliance...

77 FR 64994 - Agency Information Collection Activities; Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-24

..., or an average increase of 1,263 new businesses a year [(21,900 businesses in 2008-11,800 businesses... 2013 through 2016, the average number of established businesses during the three-year period for which.... Established Year businesses New entrants 2013 28,215 1,263 2014 29,478 1,263 2015 30,741 1,263 Average 29,478...

27 CFR 26.263 - Determination of tax on beer.

Code of Federal Regulations, 2010 CFR

2010-04-01

... beer. 26.263 Section 26.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Procedure at Port of Entry From the Virgin Islands § 26.263 Determination of tax on beer. If the certificate prescribed in § 26.205 covers beer, the beer tax will be collected on the basis of the number of barrels of...

27 CFR 26.263 - Determination of tax on beer.

Code of Federal Regulations, 2014 CFR

2014-04-01

... beer. 26.263 Section 26.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Procedure at Port of Entry From the Virgin Islands § 26.263 Determination of tax on beer. If the certificate prescribed in § 26.205 covers beer, the beer tax will be collected on the basis of the number of barrels of...

27 CFR 26.263 - Determination of tax on beer.

Code of Federal Regulations, 2013 CFR

2013-04-01

... beer. 26.263 Section 26.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Procedure at Port of Entry From the Virgin Islands § 26.263 Determination of tax on beer. If the certificate prescribed in § 26.205 covers beer, the beer tax will be collected on the basis of the number of barrels of...

27 CFR 26.263 - Determination of tax on beer.

Code of Federal Regulations, 2012 CFR

2012-04-01

... beer. 26.263 Section 26.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Procedure at Port of Entry From the Virgin Islands § 26.263 Determination of tax on beer. If the certificate prescribed in § 26.205 covers beer, the beer tax will be collected on the basis of the number of barrels of...

27 CFR 26.263 - Determination of tax on beer.

Code of Federal Regulations, 2011 CFR

2011-04-01

... beer. 26.263 Section 26.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Procedure at Port of Entry From the Virgin Islands § 26.263 Determination of tax on beer. If the certificate prescribed in § 26.205 covers beer, the beer tax will be collected on the basis of the number of barrels of...

Consolidated Checklist for C4 40 CFR 263

EPA Pesticide Factsheets

No final rules affecting 40 CFR Part 263 were promulgated between June 30, 2002 and December 31, 2002, therefore, this Consolidated Checklist corresponds to 40 CFR Part 263 as published on July 1, 2002.

40 CFR 408.263 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true [Reserved] 408.263 Section 408.263 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Atlantic and Gulf Coast Hand-Shucked Oyster...

ABT-263 induces G1/G0-phase arrest, apoptosis and autophagy in human esophageal cancer cells in vitro.

PubMed

Lin, Qing-Huan; Que, Fu-Chang; Gu, Chun-Ping; Zhong, De-Sheng; Zhou, Dan; Kong, Yi; Yu, Le; Liu, Shu-Wen

2017-12-01

Both the anti- and pro-apoptotic members of the Bcl-2 family are regulated by a conserved Bcl-2 homology (BH3) domain. ABT-263 (Navitoclax), a novel BH3 mimetic and orally bioavailable Bcl-2 family inhibitor with high affinity for Bcl-xL, Bcl-2 and Bcl-w has entered clinical trials for cancer treatment. But the anticancer mechanisms of ABT-263 have not been fully elucidated. In this study we investigated the effects of ABT-263 on human esophageal cancer cells in vitro and to explore its anticancer mechanisms. Treatment with ABT-263 dose-dependently suppressed the viability of 3 human esophageal cancer cells with IC 50 values of 10.7±1.4, 7.1±1.5 and 8.2±1.6 μmol/L, in EC109, HKESC-2 and CaES-17 cells, respectively. ABT-263 (5-20 μmol/L) dose-dependently induced G 1 /G 0 -phase arrest in the 3 cancer cell lines and induced apoptosis evidenced by increased the Annexin V-positive cell population and elevated levels of cleaved caspase 3, cleaved caspase 9 and PARP. We further demonstrated that ABT-263 treatment markedly increased the expression of p21 Waf1/Cip1 and decreased the expression of cyclin D1 and phospho-Rb (retinoblastoma tumor suppressor protein) (Ser780) proteins that contributed to the G 1 /G 0 -phase arrest. Knockdown of p21 Waf1/Cip1 attenuated ABT-263-induced G 1 /G 0 -phase arrest. Moreover, ABT-263 treatment enhanced pro-survival autophagy, shown as the increased LC3-II levels and decreased p62 levels, which counteracted its anticancer activity. Our results suggest that ABT-263 exerts cytostatic and cytotoxic effects on human esophageal cancer cells in vitro and enhances pro-survival autophagy, which counteracts its anticancer activity.

42 CFR 2.63 - Confidential communications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Confidential communications. 2.63 Section 2.63 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS... Confidential communications. (a) A court order under these regulations may authorize disclosure of confidential...

37 CFR 263.1 - General.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 37 Patents, Trademarks, and Copyrights 1 2014-07-01 2014-07-01 false General. 263.1 Section 263.1 Patents, Trademarks, and Copyrights U.S. COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION... payments for the public performance of sound recordings in certain digital transmissions by certain...

37 CFR 263.1 - General.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 37 Patents, Trademarks, and Copyrights 1 2011-07-01 2011-07-01 false General. 263.1 Section 263.1 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY... payments for the public performance of sound recordings in certain digital transmissions by certain...

37 CFR 263.1 - General.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 37 Patents, Trademarks, and Copyrights 1 2012-07-01 2012-07-01 false General. 263.1 Section 263.1 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY... payments for the public performance of sound recordings in certain digital transmissions by certain...

37 CFR 263.1 - General.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 37 Patents, Trademarks, and Copyrights 1 2013-07-01 2013-07-01 false General. 263.1 Section 263.1 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY... payments for the public performance of sound recordings in certain digital transmissions by certain...

37 CFR 263.1 - General.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false General. 263.1 Section 263.1 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY... payments for the public performance of sound recordings in certain digital transmissions by certain...

40 CFR 263.11 - EPA identification number.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 27 2013-07-01 2013-07-01 false EPA identification number. 263.11... (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.11 EPA identification number. (a) A transporter must not transport hazardous wastes without having received an EPA...

40 CFR 263.11 - EPA identification number.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 27 2012-07-01 2012-07-01 false EPA identification number. 263.11... (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.11 EPA identification number. (a) A transporter must not transport hazardous wastes without having received an EPA...

40 CFR 263.11 - EPA identification number.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 25 2010-07-01 2010-07-01 false EPA identification number. 263.11... (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.11 EPA identification number. (a) A transporter must not transport hazardous wastes without having received an EPA...

40 CFR 263.11 - EPA identification number.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 26 2014-07-01 2014-07-01 false EPA identification number. 263.11... (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.11 EPA identification number. (a) A transporter must not transport hazardous wastes without having received an EPA...

40 CFR 263.11 - EPA identification number.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 26 2011-07-01 2011-07-01 false EPA identification number. 263.11... (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.11 EPA identification number. (a) A transporter must not transport hazardous wastes without having received an EPA...

20 CFR 416.263 - No additional application needed.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false No additional application needed. 416.263 Section 416.263 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A Disabling...

21 CFR 26.3 - Scope.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Scope. 26.3 Section 26.3 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL MUTUAL RECOGNITION OF PHARMACEUTICAL GOOD MANUFACTURING PRACTICE REPORTS, MEDICAL DEVICE QUALITY SYSTEM AUDIT REPORTS, AND CERTAIN...

20 CFR 416.263 - No additional application needed.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false No additional application needed. 416.263 Section 416.263 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A Disabling...

20 CFR 416.263 - No additional application needed.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false No additional application needed. 416.263 Section 416.263 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A Disabling...

20 CFR 416.263 - No additional application needed.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false No additional application needed. 416.263 Section 416.263 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A Disabling...

26 CFR 1.263A-11 - Accumulated production expenditures.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 26 Internal Revenue 3 2011-04-01 2011-04-01 false Accumulated production expenditures. 1.263A-11... TAX (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-11 Accumulated production expenditures. (a) General rule. Accumulated production expenditures generally means the cumulative amount of...

26 CFR 1.263A-11 - Accumulated production expenditures.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 26 Internal Revenue 3 2013-04-01 2013-04-01 false Accumulated production expenditures. 1.263A-11... TAX (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-11 Accumulated production expenditures. (a) General rule. Accumulated production expenditures generally means the cumulative amount of...

26 CFR 1.263A-11 - Accumulated production expenditures.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 26 Internal Revenue 3 2014-04-01 2014-04-01 false Accumulated production expenditures. 1.263A-11... TAX (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-11 Accumulated production expenditures. (a) General rule. Accumulated production expenditures generally means the cumulative amount of...

26 CFR 1.263A-11 - Accumulated production expenditures.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 26 Internal Revenue 3 2012-04-01 2012-04-01 false Accumulated production expenditures. 1.263A-11... TAX (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-11 Accumulated production expenditures. (a) General rule. Accumulated production expenditures generally means the cumulative amount of...

50 CFR 665.263 - Prohibitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false Prohibitions. 665.263 Section 665.263 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION.... In addition to the general prohibitions specified in 50 CFR 600.725 and in 665.15, it is unlawful for...

26 CFR 1.263A-14 - Rules for related persons.

Code of Federal Regulations, 2011 CFR

2011-04-01

....263A-14 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.263A-14 Rules for related persons. Taxpayers must account for average excess expenditures allocated to related persons under applicable...

26 CFR 1.263A-14 - Rules for related persons.

Code of Federal Regulations, 2010 CFR

2010-04-01

....263A-14 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Items Not Deductible § 1.263A-14 Rules for related persons. Taxpayers must account for average excess expenditures allocated to related persons under applicable administrative...

24 CFR 26.3 - Ex parte communications.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Ex parte communications. 26.3... Development HEARING PROCEDURES Hearings Before Hearing Officers Hearing Officer § 26.3 Ex parte communications. (a) Definition. An ex parte communication is any communication with a hearing officer, direct or...

Publications - GMC 263 | Alaska Division of Geological & Geophysical

Science.gov Websites

Visiting Alaska State Employees DGGS State of Alaska search Alaska Division of Geological & Geophysical Facebook DGGS News Natural Resources Geological & Geophysical Surveys Publications GMC 263 main content DGGS GMC 263 Publication Details Title: Map location and geological logs of core for 1994 diamond drill

12 CFR 263.38 - Recommended decision and filing of record.

Code of Federal Regulations, 2010 CFR

2010-01-01

... FEDERAL RESERVE SYSTEM RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.38... expiration of the time allowed for filing reply briefs under § 263.37(b), the administrative law judge shall... the administrative law judge's recommended decision, recommended findings of fact, recommended...

12 CFR 263.53 - Discovery depositions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Discovery depositions. 263.53 Section 263.53... depositions. (a) In general. In addition to the discovery permitted in subpart A of this part, limited discovery by means of depositions shall be allowed for individuals with knowledge of facts material to the...

12 CFR 263.53 - Discovery depositions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Discovery depositions. 263.53 Section 263.53... depositions. (a) In general. In addition to the discovery permitted in subpart A of this part, limited discovery by means of depositions shall be allowed for individuals with knowledge of facts material to the...

12 CFR 263.24 - Scope of document discovery.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Scope of document discovery. 263.24 Section 263.24 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE... deliberative-process privilege, and any other privileges the Constitution, any applicable act of Congress, or...

34 CFR 263.5 - What priority is given to certain projects and applicants?

Code of Federal Regulations, 2010 CFR

2010-07-01

... an Indian institution of higher education that is eligible to participate in the Professional... applicants? 263.5 Section 263.5 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION INDIAN EDUCATION DISCRETIONARY GRANT PROGRAMS...

34 CFR 263.5 - What priority is given to certain projects and applicants?

Code of Federal Regulations, 2011 CFR

2011-07-01

... an Indian institution of higher education that is eligible to participate in the Professional... applicants? 263.5 Section 263.5 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION INDIAN EDUCATION DISCRETIONARY GRANT PROGRAMS...

12 CFR 263.102 - Prevailing party.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Prevailing party. 263.102 Section 263.102 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM RULES... Prevailing party. Only an eligible applicant that prevailed on the merits of an adversary proceeding may...

12 CFR 263.9 - Ex parte communications.

Code of Federal Regulations, 2010 CFR

2010-01-01

.... (a) Definition—(1) Ex parte communication means any material oral or written communication relevant... oral, a memorandum stating the substance of the communication) to be placed on the record of the... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Ex parte communications. 263.9 Section 263.9...

12 CFR 263.53 - Discovery depositions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Discovery depositions. 263.53 Section 263.53... Discovery depositions. (a) In general. In addition to the discovery permitted in subpart A of this part, limited discovery by means of depositions shall be allowed for individuals with knowledge of facts...

12 CFR 263.53 - Discovery depositions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Discovery depositions. 263.53 Section 263.53... Discovery depositions. (a) In general. In addition to the discovery permitted in subpart A of this part, limited discovery by means of depositions shall be allowed for individuals with knowledge of facts...

12 CFR 263.82 - Establishment of minimum capital levels.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Establishment of minimum capital levels. 263.82... Maintain Adequate Capital § 263.82 Establishment of minimum capital levels. The Board has established minimum capital levels for state member banks and bank holding companies in its Capital Adequacy...

47 CFR 1.263 - Proposed findings and conclusions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 1 2010-10-01 2010-10-01 false Proposed findings and conclusions. 1.263... Proceedings Hearing and Intermediate Decision § 1.263 Proposed findings and conclusions. (a) Each party to the proceeding may file proposed findings of fact and conclusions, briefs, or memoranda of law: Provided, however...

12 CFR 263.21 - Failure to appear.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Failure to appear. 263.21 Section 263.21 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM RULES... to the relief sought in the notice. Without further proceedings or notice to the respondent, the...

12 CFR 263.17 - Collateral attacks on adjudicatory proceeding.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Collateral attacks on adjudicatory proceeding. 263.17 Section 263.17 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE... proceeding shall continue without regard to the pendency of that court proceeding. No default or other...

27 CFR 41.263 - Maintenance of records and reports.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 2 2011-04-01 2011-04-01 false Maintenance of records and reports. 41.263 Section 41.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) TOBACCO IMPORTATION OF TOBACCO PRODUCTS, CIGARETTE PAPERS...

27 CFR 41.263 - Maintenance of records and reports.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Maintenance of records and reports. 41.263 Section 41.263 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) TOBACCO IMPORTATION OF TOBACCO PRODUCTS, CIGARETTE PAPERS...

34 CFR 263.1 - What is the Professional Development program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 1 2011-07-01 2011-07-01 false What is the Professional Development program? 263.1... Development Program § 263.1 What is the Professional Development program? (a) The Professional Development... Professional Development program requires individuals who receive training to— (1) Perform work related to the...

12 CFR 263.105 - Statement of net worth.

Code of Federal Regulations, 2010 CFR

2010-01-01

... initiation of the adversary adjudication. Unaudited financial statements are acceptable for individual... judge or the Board otherwise requires. Financial statements or reports filed with or reported to a... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Statement of net worth. 263.105 Section 263.105...

12 CFR 263.15 - Opportunity for informal settlement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Opportunity for informal settlement. 263.15 Section 263.15 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL... proposal, or any subsequent negotiation or resolution, is admissible as evidence in any proceeding. ...

40 CFR 60.263 - Standard for carbon monoxide.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Standard for carbon monoxide. 60.263... Production Facilities § 60.263 Standard for carbon monoxide. (a) On and after the date on which the... furnace any gases which contain, on a dry basis, 20 or greater volume percent of carbon monoxide...

12 CFR 263.91 - Censure, suspension or debarment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Censure, suspension or debarment. 263.91... RESERVE SYSTEM RULES OF PRACTICE FOR HEARINGS Practice Before the Board § 263.91 Censure, suspension or debarment. The Board may censure an individual or suspend or debar such individual from practice before the...

26 CFR 1.263A-5 - Exception for qualified creative expenses incurred by certain free-lance authors, photographers...

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Exception for qualified creative expenses incurred by certain free-lance authors, photographers, and artists. [Reserved] 1.263A-5 Section 1.263A-5... certain free-lance authors, photographers, and artists. [Reserved] ...

26 CFR 1.263(a)-2 - Examples of capital expenditures.

Code of Federal Regulations, 2010 CFR

2010-04-01

... the capital of the corporation for any corporate purpose. Such amounts are capital investments and are... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Examples of capital expenditures. 1.263(a)-2...) INCOME TAX (CONTINUED) INCOME TAXES Items Not Deductible § 1.263(a)-2 Examples of capital expenditures...

12 CFR 263.404 - Notice of removal, suspension, or debarment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... accountants and firms. An accountant or accounting firm that provides audit services to a banking organization... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Notice of removal, suspension, or debarment. 263.404 Section 263.404 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE...

40 CFR 60.263 - Standard for carbon monoxide.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 6 2011-07-01 2011-07-01 false Standard for carbon monoxide. 60.263... Production Facilities § 60.263 Standard for carbon monoxide. (a) On and after the date on which the... furnace any gases which contain, on a dry basis, 20 or greater volume percent of carbon monoxide...

12 CFR 263.4 - Authority of the Board.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Authority of the Board. 263.4 Section 263.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM.... The Board may, at any time during the pendency of a proceeding, perform, direct the performance of, or...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2010 CFR

2010-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false History of Program and Project...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2011 CFR

2011-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false History of Program and Project...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2013 CFR

2013-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false History of Program and Project...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2012 CFR

2012-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false History of Program and Project...

43 CFR 2.63 - Can you receive expedited processing of appeals?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false Can you receive expedited processing of appeals? 2.63 Section 2.63 Public Lands: Interior Office of the Secretary of the Interior FREEDOM OF... the need for expedited processing is true and correct to the best of your knowledge and belief. (b...

43 CFR 2.63 - Can you receive expedited processing of appeals?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false Can you receive expedited processing of appeals? 2.63 Section 2.63 Public Lands: Interior Office of the Secretary of the Interior FREEDOM OF... the need for expedited processing is true and correct to the best of your knowledge and belief. (b...

18 CFR 284.263 - Exemption from section 7 of Natural Gas Act and certain regulatory conditions.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 7 of Natural Gas Act and certain regulatory conditions. 284.263 Section 284.263 Conservation of... UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale...

26 CFR 1.263A-7 - Changing a method of accounting under section 263A.

Code of Federal Regulations, 2012 CFR

2012-04-01

... regulations thereunder and the various procedures described in this paragraph (c). (4) Anti-abuse rule—(i) In... of accounting under section 263A. (a) Introduction—(1) Purpose. These regulations provide guidance to... these regulations is to provide guidance regarding how taxpayers are to revalue property on hand at the...

12 CFR 263.1 - Scope.

Code of Federal Regulations, 2012 CFR

2012-01-01

... penalty proceedings under section 10(k) of the FDI Act (12 U.S.C. 1820(k)) for violations of the special... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Scope. 263.1 Section 263.1 Banks and Banking...-desist proceedings under section 8(b) of the Federal Deposit Insurance Act (“FDIA”) (12 U.S.C. 1818(b...

12 CFR 263.1 - Scope.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Removal, prohibition, and civil monetary penalty proceedings under section 10(k) of the FDI Act (12 U.S.C... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Scope. 263.1 Section 263.1 Banks and Banking... section 8(b) of the Federal Deposit Insurance Act (“FDIA”) (12 U.S.C. 1818(b)); (b) Removal and...

12 CFR 263.1 - Scope.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) Removal, prohibition, and civil monetary penalty proceedings under section 10(k) of the FDI Act (12 U.S.C... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Scope. 263.1 Section 263.1 Banks and Banking... section 8(b) of the Federal Deposit Insurance Act (“FDIA”) (12 U.S.C. 1818(b)); (b) Removal and...

12 CFR 263.1 - Scope.

Code of Federal Regulations, 2013 CFR

2013-01-01

... penalty proceedings under section 10(k) of the FDI Act (12 U.S.C. 1820(k)) for violations of the special... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Scope. 263.1 Section 263.1 Banks and Banking...-desist proceedings under section 8(b) of the Federal Deposit Insurance Act (“FDIA”) (12 U.S.C. 1818(b...

12 CFR 263.1 - Scope.

Code of Federal Regulations, 2014 CFR

2014-01-01

... penalty proceedings under section 10(k) of the FDI Act (12 U.S.C. 1820(k)) for violations of the special... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Scope. 263.1 Section 263.1 Banks and Banking...-desist proceedings under section 8(b) of the Federal Deposit Insurance Act (“FDIA”) (12 U.S.C. 1818(b...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false History of Program and Project Limitations Continuing Authorities Program A Appendix A to Part 263 Navigation and Navigable Waters CORPS OF... Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section...

26 CFR 1.263A-15 - Effective dates, transitional rules, and anti-abuse rule.

Code of Federal Regulations, 2010 CFR

2010-04-01

... TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Items Not Deductible § 1.263A-15 Effective dates... reasonable positions on their federal income tax returns when applying section 263A(f). For purposes of this... (relating to the capitalization of real property interest and taxes) or pursuant to an election that was in...

18 CFR 284.263 - Exemption from section 7 of Natural Gas Act and certain regulatory conditions.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 7 of Natural Gas Act and certain regulatory conditions. 284.263 Section 284.263 Conservation of... UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale...

12 CFR 263.303 - Filing of safety and soundness compliance plan.

Code of Federal Regulations, 2010 CFR

2010-01-01

... that plan, order, agreement, or response, subject to the deadline provided in paragraph (a)(1) of this... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Filing of safety and soundness compliance plan. 263.303 Section 263.303 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE...

12 CFR 263.303 - Filing of safety and soundness compliance plan.

Code of Federal Regulations, 2011 CFR

2011-01-01

... that plan, order, agreement, or response, subject to the deadline provided in paragraph (a)(1) of this... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Filing of safety and soundness compliance plan. 263.303 Section 263.303 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE...

12 CFR 263.303 - Filing of safety and soundness compliance plan.

Code of Federal Regulations, 2012 CFR

2012-01-01

... this section as part of that plan, order, agreement, or response, subject to the deadline provided in... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Filing of safety and soundness compliance plan. 263.303 Section 263.303 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE...

12 CFR 263.303 - Filing of safety and soundness compliance plan.

Code of Federal Regulations, 2014 CFR

2014-01-01

... this section as part of that plan, order, agreement, or response, subject to the deadline provided in... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Filing of safety and soundness compliance plan. 263.303 Section 263.303 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE...

12 CFR 263.303 - Filing of safety and soundness compliance plan.

Code of Federal Regulations, 2013 CFR

2013-01-01

... this section as part of that plan, order, agreement, or response, subject to the deadline provided in... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Filing of safety and soundness compliance plan. 263.303 Section 263.303 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE...

42 CFR 137.263 - May the Secretary provide a grant to a Self-Governance Tribe for technical assistance to overcome...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 137.263 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES INDIAN HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Reassumption § 137.263 May the... assistance as provided in section 103 of the Act [25 U.S.C. 458aaa-h]. ...

42 CFR 137.263 - May the Secretary provide a grant to a Self-Governance Tribe for technical assistance to overcome...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 137.263 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES INDIAN HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Reassumption § 137.263 May the... assistance as provided in section 103 of the Act [25 U.S.C. 458aaa-h]. ...

76 FR 4250 - Operating Certain Railroad Tank Cars in Excess of 263,000 Pounds Gross Rail Load; Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-25

... Railroad Tank Cars in Excess of 263,000 Pounds Gross Rail Load; Approval AGENCY: Federal Railroad... certain railroad tank cars in excess of 263,000 pounds gross rail load. SUMMARY: On May 14, 2010, the... to allow certain rail tank cars, transporting hazardous materials, to exceed the gross weight on rail...

29 CFR 779.263 - Excise taxes not at the retail level.

Code of Federal Regulations, 2010 CFR

2010-07-01

... ACT AS APPLIED TO RETAILERS OF GOODS OR SERVICES Employment to Which the Act May Apply; Enterprise Coverage Excise Taxes § 779.263 Excise taxes not at the retail level. There are also a wide variety of... 29 Labor 3 2010-07-01 2010-07-01 false Excise taxes not at the retail level. 779.263 Section 779...

31 CFR 26.3 - Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental Impact Assessments (EIAs). 26.3 Section 26.3 Money and...) 466-8191, not a toll-free call). (ii) If a copy of an EIA Summary is not available from the BIC...

31 CFR 26.3 - Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental Impact Assessments (EIAs). 26.3 Section 26.3 Money and...) 466-8191, not a toll-free call). (ii) If a copy of an EIA Summary is not available from the BIC...

31 CFR 26.3 - Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental Impact Assessments (EIAs). 26.3 Section 26.3 Money and...) 466-8191, not a toll-free call). (ii) If a copy of an EIA Summary is not available from the BIC...

31 CFR 26.3 - Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental Impact Assessments (EIAs). 26.3 Section 26.3 Money and...) 466-8191, not a toll-free call). (ii) If a copy of an EIA Summary is not available from the BIC...

31 CFR 26.3 - Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Availability of Environmental Impact Assessment Summaries (EIA Summaries) and Environmental Impact Assessments (EIAs). 26.3 Section 26.3 Money and...) 466-8191, not a toll-free call). (ii) If a copy of an EIA Summary is not available from the BIC...

29 CFR 779.263 - Excise taxes not at the retail level.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false Excise taxes not at the retail level. 779.263 Section 779... Coverage Excise Taxes § 779.263 Excise taxes not at the retail level. There are also a wide variety of taxes levied at the manufacturer's or distributor's level and not at the retail level. It should be...

29 CFR 779.263 - Excise taxes not at the retail level.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false Excise taxes not at the retail level. 779.263 Section 779... Coverage Excise Taxes § 779.263 Excise taxes not at the retail level. There are also a wide variety of taxes levied at the manufacturer's or distributor's level and not at the retail level. It should be...

29 CFR 779.263 - Excise taxes not at the retail level.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false Excise taxes not at the retail level. 779.263 Section 779... Coverage Excise Taxes § 779.263 Excise taxes not at the retail level. There are also a wide variety of taxes levied at the manufacturer's or distributor's level and not at the retail level. It should be...

29 CFR 779.263 - Excise taxes not at the retail level.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false Excise taxes not at the retail level. 779.263 Section 779... Coverage Excise Taxes § 779.263 Excise taxes not at the retail level. There are also a wide variety of taxes levied at the manufacturer's or distributor's level and not at the retail level. It should be...

43 CFR 30.263 - May a surviving spouse reserve a life estate when a tribe exercises its statutory option to...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true May a surviving spouse reserve a life estate when a tribe exercises its statutory option to purchase? 30.263 Section 30.263 Public Lands... exercises its statutory option to purchase? Yes. When the heir or devisee whose interests are subject to the...

43 CFR 30.263 - May a surviving spouse reserve a life estate when a tribe exercises its statutory option to...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false May a surviving spouse reserve a life estate when a tribe exercises its statutory option to purchase? 30.263 Section 30.263 Public Lands... exercises its statutory option to purchase? Yes. When the heir or devisee whose interests are subject to the...

43 CFR 30.263 - May a surviving spouse reserve a life estate when a tribe exercises its statutory option to...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false May a surviving spouse reserve a life estate when a tribe exercises its statutory option to purchase? 30.263 Section 30.263 Public Lands... exercises its statutory option to purchase? Yes. When the heir or devisee whose interests are subject to the...

43 CFR 30.263 - May a surviving spouse reserve a life estate when a tribe exercises its statutory option to...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false May a surviving spouse reserve a life estate when a tribe exercises its statutory option to purchase? 30.263 Section 30.263 Public Lands... exercises its statutory option to purchase? Yes. When the heir or devisee whose interests are subject to the...

43 CFR 30.263 - May a surviving spouse reserve a life estate when a tribe exercises its statutory option to...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false May a surviving spouse reserve a life estate when a tribe exercises its statutory option to purchase? 30.263 Section 30.263 Public Lands... exercises its statutory option to purchase? Yes. When the heir or devisee whose interests are subject to the...

77 FR 74583 - Guidance Regarding Deduction and Capitalization of Expenditures Related to Tangible Property

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-17

...)-1T, 1.168(i)-7T, 1.168(i)-8T, 1.263A-1T, and 1.1016-3T, Kathleen Reed or Patrick Clinton, Office... 162, 165, 167,168, 263, 263A, and 1016 of the Internal Revenue Code. The temporary regulations (TD..., 2012. * * * * * 0 Par. 10. Section 1.168(i)-7T is amended by: 0 1. Revising paragraph (e)(1). 0 2...

Characteristics of 263K Scrapie Agent in Multiple Hamster Species

PubMed Central

Barbian, Kent D.; Race, Brent; Favara, Cynthia; Gardner, Don; Taubner, Lara; Porcella, Stephen; Race, Richard

2009-01-01

Transmissible spongiform encephalopathy (TSE) diseases are known to cross species barriers, but the pathologic and biochemical changes that occur during transmission are not well understood. To better understand these changes, we infected 6 hamster species with 263K hamster scrapie strain and, after each of 3 successive passages in the new species, analyzed abnormal proteinase K (PK)–resistant prion protein (PrPres) glycoform ratios, PrPres PK sensitivity, incubation periods, and lesion profiles. Unique 263K molecular and biochemical profiles evolved in each of the infected hamster species. Characteristics of 263K in the new hamster species seemed to correlate best with host factors rather than agent strain. Furthermore, 2 polymorphic regions of the prion protein amino acid sequence correlated with profile differences in these TSE-infected hamster species. PMID:19193264

29 CFR 500.263 - Authority of the Secretary.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Administrative Law Judge § 500.263 Authority of the Secretary. The Secretary may modify or vacate the Decision... inconsistent with a policy or precedent established by the Department of Labor, (b) Encompasses determinations...

50 CFR 648.263 - Red crab possession and landing restrictions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE NORTHEASTERN UNITED STATES Management Measures for the Atlantic Deep-Sea Red Crab Fishery § 648.263 Red crab possession and landing...

Structural and Biochemical Characterization of Chlamydia trachomatis Hypothetical Protein CT263 Supports That Menaquinone Synthesis Occurs through the Futalosine Pathway*

PubMed Central

Barta, Michael L.; Thomas, Keisha; Yuan, Hongling; Lovell, Scott; Battaile, Kevin P.; Schramm, Vern L.; Hefty, P. Scott

2014-01-01

The obligate intracellular human pathogen Chlamydia trachomatis is the etiological agent of blinding trachoma and sexually transmitted disease. Genomic sequencing of Chlamydia indicated this medically important bacterium was not exclusively dependent on the host cell for energy. In order for the electron transport chain to function, electron shuttling between membrane-embedded complexes requires lipid-soluble quinones (e.g. menaquionone or ubiquinone). The sources or biosynthetic pathways required to obtain these electron carriers within C. trachomatis are poorly understood. The 1.58Å crystal structure of C. trachomatis hypothetical protein CT263 presented here supports a role in quinone biosynthesis. Although CT263 lacks sequence-based functional annotation, the crystal structure of CT263 displays striking structural similarity to 5′-methylthioadenosine nucleosidase (MTAN) enzymes. Although CT263 lacks the active site-associated dimer interface found in prototypical MTANs, co-crystal structures with product (adenine) or substrate (5′-methylthioadenosine) indicate that the canonical active site residues are conserved. Enzymatic characterization of CT263 indicates that the futalosine pathway intermediate 6-amino-6-deoxyfutalosine (kcat/Km = 1.8 × 103 m−1 s−1), but not the prototypical MTAN substrates (e.g. S-adenosylhomocysteine and 5′-methylthioadenosine), is hydrolyzed. Bioinformatic analyses of the chlamydial proteome also support the futalosine pathway toward the synthesis of menaquinone in Chlamydiaceae. This report provides the first experimental support for quinone synthesis in Chlamydia. Menaquinone synthesis provides another target for agents to combat C. trachomatis infection. PMID:25253688

Development of an Atmospheric Dispersion Model for Heavier-Than-Air Gas Mixtures. Volume 1.

DTIC Science & Technology

1985-05-01

aspirated concentration sensor used a balanced Wheatstone bridge to measure the heat loss from a sensing element placed in the sample stream. Shaded...a semipermeable membrane and electrochemical cell. A fast response sensor (10 Hz) basically aspirated a sample past the cell membrane. Reported...ramp function around the freezing point of water by X11., X= vap for T 273.15 K vap fus 263.15 for 263.15 <T < 273.15 Xvap +x fus for T < 263.15 (A-4

LkHα 262/263: the paradigm of multiplicity vs disk fraction in low-mass stellar systems.

NASA Astrophysics Data System (ADS)

Velasco, S.; Rebolo, R.; Oscoz, A.; Labadie, L.; Pérez-Garrido, A.

2017-03-01

The study of multiple systems and their link with the presence of discs around their components is key to understanding the evolution of low-mass pre-main sequence stars. Although there are indications that high-multiplicity systems are much more frequent among very young stars, until now, only a few of these young low-mass stellar systems have been confirmed. Here, we present high spatial resolution i band imaging of the system formed by LkHα 262 and LkHα 263, in the MBM12 cloud. It was obtained during the first commissioning period of the Adaptive Optics Lucky Imager (AOLI) at the 4.2 m William Herschel Telescope, using its Lucky Imaging mode. The multiple system LkHα 262/263 is composed of four low-mass very young M-type stars and some discs, including an edge-on disc around LkHα 263C. The AOLI data combined with previously available and newly obtained optical and infrared imaging show that the three components of LkHα 263 are co-moving, that there is orbital motion in the AB pair (0.41arcsec separation), and, remarkably, that LkHα 262-263 is a common proper motion system with a less than 1 mas/yr relative motion. According to BT-settl models the mass of each of the five components is close to 0.4 M and the age is in the range 1-2 Myr. We also give marginal evidence of a cooler companion to LkHα 262, at less than 0.15 arcsec, turning LkHα 262-263 into a five-component likely gravitationally bounded system. The presence of discs in some of the components offers an interesting opportunity to investigate the formation and evolution of discs in the early stages of multiple very low-mass systems.

PI3K and Bcl-2 inhibition primes glioblastoma cells to apoptosis through downregulation of Mcl-1 and Phospho-BAD.

PubMed

Pareja, Fresia; Macleod, David; Shu, Chang; Crary, John F; Canoll, Peter D; Ross, Alonzo H; Siegelin, Markus D

2014-07-01

Glioblastoma multiforme (GBM) is a highly malignant human brain neoplasm with limited therapeutic options. GBMs display a deregulated apoptotic pathway with high levels of the antiapoptotic Bcl-2 family of proteins and overt activity of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. Therefore, combined interference of the PI3K pathway and the Bcl-2 family of proteins is a reasonable therapeutic strategy. ABT-263 (Navitoclax), an orally available small-molecule Bcl-2 inhibitor, and GDC-0941, a PI3K inhibitor, were used to treat established glioblastoma and glioblastoma neurosphere cells, alone or in combination. Although GDC-0941 alone had a modest effect on cell viability, treatment with ABT-263 displayed a marked reduction of cell viability and induction of apoptotic cell death. Moreover, combinatorial therapy using ABT-263 and GDC-0941 showed an enhanced effect, with a further decrease in cellular viability. Furthermore, combination treatment abrogated the ability of stem cell-like glioma cells to form neurospheres. ABT-263 and GDC-0941, in combination, resulted in a consistent and significant increase of Annexin V positive cells and loss of mitochondrial membrane potential compared with either monotherapy. The combination treatment led to enhanced cleavage of both initiator and effector caspases. Mechanistically, GDC-0941 depleted pAKT (Serine 473) levels and suppressed Mcl-1 protein levels, lowering the threshold for the cytotoxic actions of ABT-263. GDC-0941 decreased Mcl-1 in a posttranslational manner and significantly decreased the half-life of Mcl-1 protein. Ectopic expression of human Mcl-1 mitigated apoptotic cell death induced by the drug combination. Furthermore, GDC-0941 modulated the phosphorylation status of BAD, thereby further enhancing ABT-263-mediated cell death. Combination therapy with ABT-263 and GDC-0941 has novel therapeutic potential by specifically targeting aberrantly active, deregulated pathways in GBM, overcoming endogenous resistance to apoptosis. ©2014 American Association for Cancer Research.

Molecular cytogenetic characterization of Xp22.32→pter deletion and Xq26.3→qter duplication in a male fetus associated with 46,Y,rec(X)dup(Xq) inv(X)(p22.3q26.3), a hypoplastic left heart, short stature, and maternal X chromosome pericentric inversion.

PubMed

Chen, Chih-Ping; Chen, Chen-Yu; Chern, Schu-Rern; Wu, Peih-Shan; Chen, Yen-Ni; Chen, Shin-Wen; Lee, Chen-Chi; Town, Dai-Dyi; Lee, Meng-Shan; Yang, Chien-Wen; Wang, Wayseen

2016-10-01

We present molecular cytogenetic characterization of an Xp22.32→pter deletion and an Xq26.3→qter duplication in a male fetus with congenital malformations and maternal X chromosome pericentric inversion. A 22-year-old woman underwent amniocentesis at 17 weeks of gestation because of an abnormal maternal serum screening result. Prenatal ultrasound revealed a hypoplastic left heart and short limbs. Amniocentesis revealed a karyotype of 46,Y,der(X) t(X;?)(p22.31;?). The pregnancy was subsequently terminated, and a malformed fetus was delivered with short stature and facial dysmorphism. Repeat amniocentesis was performed before termination of the pregnancy. Array comparative genomic hybridization was performed on uncultured amniocytes and maternal blood. Conventional cytogenetic analysis was performed on cultured amniocytes, cord blood, and blood from both parents. Fluorescence in situ hybridization was performed on cultured amniocytes. The maternal karyotype was 46,X,inv(X)(p22.3q26.3). The fetal karyotype was 46,Y, rec(X)dup(Xq)inv(X)(p22.3q26.3) or 46,Y, rec(X)(qter→q26.3::p22.3→qter). Array comparative genomic hybridization on uncultured amniocytes revealed a 4.56-Mb deletion of Xp22.33-p22.32 encompassing SHOX, CSF2RA, and ARSE, and a 19.22-Mb duplication of Xq26.3-q28 encompassing SOX3, FMR1, MECP2, RAB39B, and CLIC2 in the fetus. The mother did not have X chromosome imbalance. Detection of X chromosome aberration in a male fetus should give suspicion of a recombinant X chromosome derived from maternal X chromosome pericentric inversion. Copyright © 2016. Published by Elsevier B.V.

26 CFR 1.263(a)-0 - Table of contents.

Code of Federal Regulations, 2010 CFR

2010-04-01

...) adjustment. § 1.263(a)-5Amounts paid or incurred to facilitate an acquisition of a trade or business, a... costs. (5) Stock issuance costs of open-end regulated investment companies. (6) Integration costs. (7...

34 CFR 263.7 - What are the requirements for a leave of absence?

Code of Federal Regulations, 2011 CFR

2011-07-01

... SECONDARY EDUCATION, DEPARTMENT OF EDUCATION INDIAN EDUCATION DISCRETIONARY GRANT PROGRAMS Professional Development Program § 263.7 What are the requirements for a leave of absence? (a) A participant shall submit a...

34 CFR 263.7 - What are the requirements for a leave of absence?

Code of Federal Regulations, 2010 CFR

2010-07-01

... SECONDARY EDUCATION, DEPARTMENT OF EDUCATION INDIAN EDUCATION DISCRETIONARY GRANT PROGRAMS Professional Development Program § 263.7 What are the requirements for a leave of absence? (a) A participant shall submit a...

A large Indian family with rearrangement of chromosome 4p16 and 3p26.3 and divergent clinical presentations.

PubMed

Iype, Thomas; Alakbarzade, Vafa; Iype, Mary; Singh, Royana; Sreekantan-Nair, Ajith; Chioza, Barry A; Mohapatra, Tribhuvan M; Baple, Emma L; Patton, Michael A; Warner, Thomas T; Proukakis, Christos; Kulkarni, Abhi; Crosby, Andrew H

2015-11-10

The deletion of the chromosome 4p16.3 Wolf-Hirschhorn syndrome critical region (WHSCR-2) typically results in a characteristic facial appearance, varying intellectual disability, stereotypies and prenatal onset of growth retardation, while gains of the same chromosomal region result in a more variable degree of intellectual deficit and dysmorphism. Similarly the phenotype of individuals with terminal deletions of distal chromosome 3p (3p deletion syndrome) varies from mild to severe intellectual deficit, micro- and trigonocephaly, and a distinct facial appearance. We investigated a large Indian five-generation pedigree with ten affected family members in which chromosomal microarray and fluorescence in situ hybridization analyses disclosed a complex rearrangement involving chromosomal subregions 4p16.1 and 3p26.3 resulting in a 4p16.1 deletion and 3p26.3 microduplication in three individuals, and a 4p16.1 duplication and 3p26.3 microdeletion in seven individuals. A typical clinical presentation of WHS was observed in all three cases with 4p16.1 deletion and 3p26.3 microduplication. Individuals with a 4p16.1 duplication and 3p26.3 microdeletion demonstrated a range of clinical features including typical 3p microdeletion or 4p partial trisomy syndrome to more severe neurodevelopmental delay with distinct dysmorphic features. We present the largest pedigree with complex t(4p;3p) chromosomal rearrangements and diverse clinical outcomes including Wolf Hirschorn-, 3p deletion-, and 4p duplication syndrome amongst affected individuals.

Anticancer compound ABT-263 accelerates apoptosis in virus-infected cells and imbalances cytokine production and lowers survival rates of infected mice.

PubMed

Kakkola, L; Denisova, O V; Tynell, J; Viiliäinen, J; Ysenbaert, T; Matos, R C; Nagaraj, A; Ohman, T; Kuivanen, S; Paavilainen, H; Feng, L; Yadav, B; Julkunen, I; Vapalahti, O; Hukkanen, V; Stenman, J; Aittokallio, T; Verschuren, E W; Ojala, P M; Nyman, T; Saelens, X; Dzeyk, K; Kainov, D E

2013-07-25

ABT-263 and its structural analogues ABT-199 and ABT-737 inhibit B-cell lymphoma 2 (Bcl-2), BCL2L1 long isoform (Bcl-xL) and BCL2L2 (Bcl-w) proteins and promote cancer cell death. Here, we show that at non-cytotoxic concentrations, these small molecules accelerate the deaths of non-cancerous cells infected with influenza A virus (IAV) or other viruses. In particular, we demonstrate that ABT-263 altered Bcl-xL interactions with Bcl-2 antagonist of cell death (Bad), Bcl-2-associated X protein (Bax), uveal autoantigen with coiled-coil domains and ankyrin repeats protein (UACA). ABT-263 thereby activated the caspase-9-mediated mitochondria-initiated apoptosis pathway, which, together with the IAV-initiated caspase-8-mediated apoptosis pathway, triggered the deaths of IAV-infected cells. Our results also indicate that Bcl-xL, Bcl-2 and Bcl-w interact with pattern recognition receptors (PRRs) that sense virus constituents to regulate cellular apoptosis. Importantly, premature killing of IAV-infected cells by ABT-263 attenuated the production of key pro-inflammatory and antiviral cytokines. The imbalance in cytokine production was also observed in ABT-263-treated IAV-infected mice, which resulted in an inability of the immune system to clear the virus and eventually lowered the survival rates of infected animals. Thus, the results suggest that the chemical inhibition of Bcl-xL, Bcl-2 and Bcl-w could potentially be hazardous for cancer patients with viral infections.

12 CFR 263.16 - The Board's right to conduct examination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... FEDERAL RESERVE SYSTEM RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.16 The... or continue any form of investigation authorized by law. [56 FR 38052, Aug. 9, 1991; 56 FR 60056, Nov...

40 CFR 263.31 - Discharge clean up.

Code of Federal Regulations, 2010 CFR

2010-07-01

... hazardous waste discharge no longer presents a hazard to human health or the environment. ....31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.31 Discharge clean...

40 CFR 263.31 - Discharge clean up.

Code of Federal Regulations, 2014 CFR

2014-07-01

... hazardous waste discharge no longer presents a hazard to human health or the environment. ....31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.31 Discharge clean...

40 CFR 263.10 - Scope.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.10 Scope. (a) These regulations establish standards which apply to persons transporting hazardous waste within the United States if the transportation...

40 CFR 263.31 - Discharge clean up.

Code of Federal Regulations, 2013 CFR

2013-07-01

... hazardous waste discharge no longer presents a hazard to human health or the environment. ....31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.31 Discharge clean...

40 CFR 263.31 - Discharge clean up.

Code of Federal Regulations, 2011 CFR

2011-07-01

... hazardous waste discharge no longer presents a hazard to human health or the environment. ....31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.31 Discharge clean...

40 CFR 263.10 - Scope.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.10 Scope. (a) These regulations establish standards which apply to persons transporting hazardous waste within the United States if the transportation...

40 CFR 263.31 - Discharge clean up.

Code of Federal Regulations, 2012 CFR

2012-07-01

... hazardous waste discharge no longer presents a hazard to human health or the environment. ....31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.31 Discharge clean...

40 CFR 263.10 - Scope.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.10 Scope. (a) These regulations establish standards which apply to persons transporting hazardous waste within the United States if the transportation...

40 CFR 263.10 - Scope.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE General § 263.10 Scope. (a) These regulations establish standards which apply to persons transporting hazardous waste within the United States if the transportation...

40 CFR 263.30 - Immediate action.

Code of Federal Regulations, 2014 CFR

2014-07-01

... responsibilities determines that immediate removal of the waste is necessary to protect human health or the....30 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.30 Immediate action...

40 CFR 263.30 - Immediate action.

Code of Federal Regulations, 2013 CFR

2013-07-01

... responsibilities determines that immediate removal of the waste is necessary to protect human health or the....30 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.30 Immediate action...

40 CFR 263.30 - Immediate action.

Code of Federal Regulations, 2010 CFR

2010-07-01

... responsibilities determines that immediate removal of the waste is necessary to protect human health or the....30 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.30 Immediate action...

40 CFR 263.30 - Immediate action.

Code of Federal Regulations, 2011 CFR

2011-07-01

... responsibilities determines that immediate removal of the waste is necessary to protect human health or the....30 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.30 Immediate action...

40 CFR 263.30 - Immediate action.

Code of Federal Regulations, 2012 CFR

2012-07-01

... responsibilities determines that immediate removal of the waste is necessary to protect human health or the....30 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Hazardous Waste Discharges § 263.30 Immediate action...

Possibilities of production of transfermium nuclei in complete fusion reactions with radioactive beams

NASA Astrophysics Data System (ADS)

Hong, Juhee; Adamian, G. G.; Antonenko, N. V.

2017-07-01

The possibilities of direct production of new isotopes of transfermium nuclei 261,263,264No, 264Lr263, 263,264,266,268Rf, 265Db264, and 267,268,270,272Sg are studied in various asymmetric hot fusion-evaporation reactions with radioactive beams. The optimal reaction partners and conditions for the synthesis of new isotopes are suggested. The products of the suggested reactions can fill a gap of unknown isotopes between the isotopes of heaviest nuclei obtained in the x n evaporation channels of the cold and hot complete fusion reactions with the stable beams.

42 CFR 2.63 - Confidential communications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS Court Orders Authorizing Disclosure and Use § 2.63... bodily injury, including circumstances which constitute suspected child abuse and neglect and verbal... injury, including homicide, rape, kidnapping, armed robbery, assault with a deadly weapon, or child abuse...

42 CFR 2.63 - Confidential communications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS Court Orders Authorizing Disclosure and Use § 2.63... bodily injury, including circumstances which constitute suspected child abuse and neglect and verbal... injury, including homicide, rape, kidnapping, armed robbery, assault with a deadly weapon, or child abuse...

42 CFR 2.63 - Confidential communications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS Court Orders Authorizing Disclosure and Use § 2.63... bodily injury, including circumstances which constitute suspected child abuse and neglect and verbal... injury, including homicide, rape, kidnapping, armed robbery, assault with a deadly weapon, or child abuse...

42 CFR 2.63 - Confidential communications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS Court Orders Authorizing Disclosure and Use § 2.63... bodily injury, including circumstances which constitute suspected child abuse and neglect and verbal... injury, including homicide, rape, kidnapping, armed robbery, assault with a deadly weapon, or child abuse...

34 CFR 263.9 - When does payback begin?

Code of Federal Regulations, 2011 CFR

2011-07-01

..., DEPARTMENT OF EDUCATION INDIAN EDUCATION DISCRETIONARY GRANT PROGRAMS Professional Development Program § 263... Professional Development program, payback shall begin within six months from the date of completion of the training. (b) For participants who do not complete their training under the Professional Development...

40 CFR 263.22 - Recordkeeping.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Compliance With the Manifest System and Recordkeeping § 263.22 Recordkeeping. (a) A transporter of hazardous waste must keep a copy of the manifest signed by the generator...

40 CFR 263.22 - Recordkeeping.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO TRANSPORTERS OF HAZARDOUS WASTE Compliance With the Manifest System and Recordkeeping § 263.22 Recordkeeping. (a) A transporter of hazardous waste must keep a copy of the manifest signed by the generator...

Outbreak of Invasive Infections From Subtype emm26.3 Group A Streptococcus Among Homeless Adults-Anchorage, Alaska, 2016-2017.

PubMed

Mosites, Emily; Frick, Anna; Gounder, Prabhu; Castrodale, Louisa; Li, Yuan; Rudolph, Karen; Hurlburt, Debby; Lecy, Kristen D; Zulz, Tammy; Adebanjo, Tolu; Onukwube, Jennifer; Beall, Bernard; Van Beneden, Chris A; Hennessy, Thomas; McLaughlin, Joseph; Bruce, Michael G

2018-03-19

In 2016, we detected an outbreak of group A Streptococcus (GAS) invasive infections among the estimated 1000 persons experiencing homelessness (PEH) in Anchorage, Alaska. We characterized the outbreak and implemented a mass antibiotic intervention at homeless service facilities. We identified cases through the Alaska GAS laboratory-based surveillance system. We conducted emm typing, antimicrobial susceptibility testing, and whole-genome sequencing on all invasive isolates and compared medical record data of patients infected with emm26.3 and other emm types. In February 2017, we offered PEH at 6 facilities in Anchorage a single dose of 1 g of azithromycin. We collected oropharyngeal and nonintact skin swabs on a subset of participants concurrent with the intervention and 4 weeks afterward. From July 2016 through April 2017, we detected 42 invasive emm26.3 cases in Anchorage, 35 of which were in PEH. The emm26.3 isolates differed on average by only 2 single-nucleotide polymorphisms. Compared to other emm types, infection with emm26.3 was associated with cellulitis (odds ratio [OR], 2.5; P = .04) and necrotizing fasciitis (OR, 4.4; P = .02). We dispensed antibiotics to 391 PEH. Colonization with emm26.3 decreased from 4% of 277 at baseline to 1% of 287 at follow-up (P = .05). Invasive GAS incidence decreased from 1.5 cases per 1000 PEH/week in the 6 weeks prior to the intervention to 0.2 cases per 1000 PEH/week in the 6 weeks after (P = .01). In an invasive GAS outbreak in PEH in Anchorage, mass antibiotic administration was temporally associated with reduced invasive disease cases and colonization prevalence.

12 CFR 26.3 - Prohibitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... total assets of $50 million or more. (c) Major assets. A management official of a depository... Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY MANAGEMENT OFFICIAL INTERLOCKS § 26.3 Prohibitions. (a) Community. A management official of a depository organization may not serve at the same time...

CW and pulsed electrically detected magnetic resonance spectroscopy at 263 GHz/12 T on operating amorphous silicon solar cells

NASA Astrophysics Data System (ADS)

Akhtar, W.; Schnegg, A.; Veber, S.; Meier, C.; Fehr, M.; Lips, K.

2015-08-01

Here we describe a new high frequency/high field continuous wave and pulsed electrically detected magnetic resonance (CW EDMR and pEDMR) setup, operating at 263 GHz and resonance fields between 0 and 12 T. Spin dependent transport in illuminated hydrogenated amorphous silicon p-i-n solar cells at 5 K and 90 K was studied by in operando 263 GHz CW and pEDMR alongside complementary X-band CW EDMR. Benefiting from the superior resolution at 263 GHz, we were able to better resolve EDMR signals originating from spin dependent hopping and recombination processes. 5 K EDMR spectra were found to be dominated by conduction and valence band tail states involved in spin dependent hopping, with additional contributions from triplet exciton states. 90 K EDMR spectra could be assigned to spin pair recombination involving conduction band tail states and dangling bonds as the dominating spin dependent transport process, with additional contributions from valence band tail and triplet exciton states.

26 CFR 1.263A-0 - Outline of regulations under section 263A.

Code of Federal Regulations, 2014 CFR

2014-04-01

...) In general. (ii) Pre-production costs. (iii) Post-production costs. (4) Practical capacity concept...) Taxes. (M) Insurance. (N) Utilities. (O) Repairs and maintenance. (P) Engineering and design costs. (Q... processing services. (E) Engineering and design services. (F) Safety engineering services. (v) Accounting...

14 CFR 121.263 - Fire-extinguishing systems.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 121.263 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... the airplane in case of fire is provided by the use of fireproof materials in the nacelle and other... designated fire zones. (b) Materials in the fire-extinguishing system must not react chemically with the...

14 CFR 121.263 - Fire-extinguishing systems.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 121.263 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... the airplane in case of fire is provided by the use of fireproof materials in the nacelle and other... designated fire zones. (b) Materials in the fire-extinguishing system must not react chemically with the...

26 CFR 1.263A-1 - Uniform capitalization of costs.

Code of Federal Regulations, 2014 CFR

2014-04-01

... or facilities. (P) Engineering and design costs. Engineering and design costs include pre-production costs, such as costs attributable to research, experimental, engineering, and design activities (to the... customer demand. (9) Research and experimental expenditures. See section 263A(c)(2) for an exception for...

Effects of storage environment on the moisture content and microbial growth of food waste.

PubMed

Chen, Ying-Chu; Hsu, Yi-Cheng; Wang, Chung-Ting

2018-05-15

Food waste (FW) has become a critical issue in sustainable development as the world's population has increased. Direct incineration of FW remains the primary treatment option. The moisture content of FW may affect the energy efficiency of incineration. In Taiwan, FW, which includes raw (r-FW) and post-consumer (p-FW) waste, is often stored in freezers before pretreatment. This study evaluated the effects of storage environment on the moisture content and microbial growth of FW. Storage at 263 K was associated with the largest reduction in moisture content in both r-FW and p-FW. At 263 K, the moisture content of r-FW and p-FW was lowest at 96 and 72 h, respectively. The E.coli and total bacteria counts were steady over 120 h when stored at 263 K. Storage at 253 K required the greatest electricity consumption, followed by 263 K and 258 K. Based on the reduction of moisture content and increase in energy efficiency, it is suggested that FW is placed in temporary storage at 263 K before (pre)treatment. The results of this study will help waste-to-energy plants, incinerators, and waste management enterprises to implement proper (pre)treatment of FW for sustainable waste management. Copyright © 2018 Elsevier Ltd. All rights reserved.

Carbohydrates and activity of natural and recombinant tissue factor.

PubMed

Krudysz-Amblo, Jolanta; Jennings, Mark E; Mann, Kenneth G; Butenas, Saulius

2010-01-29

The effect of glycosylation on tissue factor (TF) activity was evaluated, and site-specific glycosylation of full-length recombinant TF (rTF) and that of natural TF from human placenta (pTF) were studied by liquid chromatography-tandem mass spectrometry. The amidolytic activity of the TF.factor VIIa (FVIIa) complex toward a fluorogenic substrate showed that the catalytic efficiency (V(max)) of the complex increased in the order rTF(1-243) (Escherichia coli) < rTF(1-263) (Sf9 insect cells) < pTF for the glycosylated and deglycosylated forms. Substrate hydrolysis was unaltered by deglycosylation. In FXase, the K(m) of FX for rTF(1-263)-FVIIa remained unchanged after deglycosylation, whereas the k(cat) decreased slightly. A pronounced decrease, 4-fold, in k(cat) was observed for pTF.FVIIa upon deglycosylation, whereas the K(m) was minimally altered. The parameters of FX activation by both rTF(1-263D)-FVIIa and pTF(D)-FVIIa were identical and similar to those for rTF(1-243)-FVIIa. In conclusion, carbohydrates significantly influence the activity of TF proteins. Carbohydrate analysis revealed glycosylation on asparagines 11, 124, and 137 in both rTF(1-263) and pTF. The carbohydrates of rTF(1-263) contain high mannose, hybrid, and fucosylated glycans. Natural pTF contains no high mannose glycans but is modified with hybrid, highly fucosylated, and sialylated sugars.

26 CFR 1.263A-0 - Outline of regulations under section 263A.

Code of Federal Regulations, 2010 CFR

2010-04-01

...) Utilities. (O) Repairs and maintenance. (P) Engineering and design costs. (Q) Spoilage. (R) Tools and...) Engineering and design services. (F) Safety engineering services. (v) Accounting method change. (h) Simplified... mixed service costs. (7) Costs allocable to more than one business. (8) De minimis rule. (9) Separate...

26 CFR 1.263A-0 - Outline of regulations under section 263A.

Code of Federal Regulations, 2012 CFR

2012-04-01

...) Utilities. (O) Repairs and maintenance. (P) Engineering and design costs. (Q) Spoilage. (R) Tools and...) Engineering and design services. (F) Safety engineering services. (v) Accounting method change. (h) Simplified... mixed service costs. (7) Costs allocable to more than one business. (8) De minimis rule. (9) Separate...

26 CFR 1.263A-0 - Outline of regulations under section 263A.

Code of Federal Regulations, 2011 CFR

2011-04-01

...) Utilities. (O) Repairs and maintenance. (P) Engineering and design costs. (Q) Spoilage. (R) Tools and...) Engineering and design services. (F) Safety engineering services. (v) Accounting method change. (h) Simplified... mixed service costs. (7) Costs allocable to more than one business. (8) De minimis rule. (9) Separate...

26 CFR 1.263A-0 - Outline of regulations under section 263A.

Code of Federal Regulations, 2013 CFR

2013-04-01

...) Utilities. (O) Repairs and maintenance. (P) Engineering and design costs. (Q) Spoilage. (R) Tools and...) Engineering and design services. (F) Safety engineering services. (v) Accounting method change. (h) Simplified... mixed service costs. (7) Costs allocable to more than one business. (8) De minimis rule. (9) Separate...

263. Photocopy of drawing (1977 piping drawing by StearnsRoger Incorporated) ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

263. Photocopy of drawing (1977 piping drawing by Stearns-Roger Incorporated) NOAA MODIFICATION, SECTIONS FOR NITROGEN SUPPLY CABINET FOR INERTIAL MEASUREMENT UNIT, SHEET 501-P8 - Vandenberg Air Force Base, Space Launch Complex 3, Launch Pad 3 East, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

32 CFR 263.10 - Parking.

Code of Federal Regulations, 2010 CFR

2010-07-01

... VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.10 Parking. (a) No person, unless otherwise authorized by a posted traffic sign or directed by a uniformed guard, shall stand or park a motor vehicle: (1) On a sidewalk, lawn, plants or shrubs. (2) Within an intersection or within a crosswalk. (3) Within...

32 CFR 263.10 - Parking.

Code of Federal Regulations, 2011 CFR

2011-07-01

... VEHICLE CONTROL ON CERTAIN DEFENSE MAPPING AGENCY SITES § 263.10 Parking. (a) No person, unless otherwise authorized by a posted traffic sign or directed by a uniformed guard, shall stand or park a motor vehicle: (1) On a sidewalk, lawn, plants or shrubs. (2) Within an intersection or within a crosswalk. (3) Within...

12 CFR 263.402 - Removal, suspension, or debarment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... accountant from performing audit services for banking organizations that are subject to section 36 of the..., immediately suspend such accountant or firm from performing audit services for banking organizations, if the... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Removal, suspension, or debarment. 263.402...

26 CFR 1.263A-1 - Uniform capitalization of costs.

Code of Federal Regulations, 2010 CFR

2010-04-01

... these terms and conditions, the principles of § 1.263A-7 must be applied in revaluing inventory property... tax principles. In determining whether a taxpayer is a bona-fide service provider under this paragraph... costs are marketing, selling, advertising, and distribution costs. (B) Research and experimental...

33 CFR 263.17 - Planning, design and construction procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... recommendations. Public notices are not required when a feasibility study is terminated without submittal of a DPR...) WASH DC 20314, depending on the study authority, as provided for in § 263.16(a). (a) Initiation of... sufficient justification for authorizing a feasibility study. Reporting officers are to notify the Division...

33 CFR 263.17 - Planning, design and construction procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

... recommendations. Public notices are not required when a feasibility study is terminated without submittal of a DPR...) WASH DC 20314, depending on the study authority, as provided for in § 263.16(a). (a) Initiation of... sufficient justification for authorizing a feasibility study. Reporting officers are to notify the Division...

34 CFR 263.3 - What definitions apply to the Professional Development program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 1 2011-07-01 2011-07-01 false What definitions apply to the Professional Development... Professional Development Program § 263.3 What definitions apply to the Professional Development program? The following definitions apply to the Professional Development program: Bureau-funded school means a Bureau...

34 CFR 263.4 - What training costs may a Professional Development program include?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false What training costs may a Professional Development... GRANT PROGRAMS Professional Development Program § 263.4 What training costs may a Professional Development program include? (a) A Professional Development program may include, as training costs, assistance...

34 CFR 263.4 - What training costs may a Professional Development program include?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 1 2011-07-01 2011-07-01 false What training costs may a Professional Development... GRANT PROGRAMS Professional Development Program § 263.4 What training costs may a Professional Development program include? (a) A Professional Development program may include, as training costs, assistance...

26 CFR 1.263(a)-0 - Outline of regulations under section 263(a).

Code of Federal Regulations, 2014 CFR

2014-04-01

... amounts paid for improvements. (e) Determining the unit of property. (1) In general. (2) Building. (i) In general. (ii) Application of improvement rules to a building. (A) Building structure. (B) Building system. (iii) Condominium. (A) In general. (B) Application of improvement rules to a condominium. (iv...

26 CFR 1.263A-1 - Uniform capitalization of costs.

Code of Federal Regulations, 2013 CFR

2013-04-01

... costs. Engineering and design costs include pre-production costs, such as costs attributable to research, experimental, engineering, and design activities (to the extent that such amounts are not research and... customer demand. (9) Research and experimental expenditures. See section 263A(c)(2) for an exception for...

26 CFR 1.263A-1 - Uniform capitalization of costs.

Code of Federal Regulations, 2011 CFR

2011-04-01

... costs. Engineering and design costs include pre-production costs, such as costs attributable to research, experimental, engineering, and design activities (to the extent that such amounts are not research and... customer demand. (9) Research and experimental expenditures. See section 263A(c)(2) for an exception for...

26 CFR 1.263A-1 - Uniform capitalization of costs.

Code of Federal Regulations, 2012 CFR

2012-04-01

... costs. Engineering and design costs include pre-production costs, such as costs attributable to research, experimental, engineering, and design activities (to the extent that such amounts are not research and... customer demand. (9) Research and experimental expenditures. See section 263A(c)(2) for an exception for...

12 CFR 263.403 - Automatic removal, suspension, and debarment.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Accountants From Performing Audit Services § 263.403 Automatic removal, suspension, and debarment. (a) An independent public accountant or accounting firm may not perform audit services for banking organizations if the accountant or firm: (1) Is subject to a final order of removal, suspension, or debarment (other...

12 CFR 263.403 - Automatic removal, suspension, and debarment.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Accountants From Performing Audit Services § 263.403 Automatic removal, suspension, and debarment. (a) An independent public accountant or accounting firm may not perform audit services for banking organizations if the accountant or firm: (1) Is subject to a final order of removal, suspension, or debarment (other...

12 CFR 263.404 - Notice of removal, suspension, or debarment.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Accountants From Performing Audit Services § 263.404 Notice of removal, suspension, or debarment. (a) Notice... independent public accountant or accounting firm from providing audit services, the Board shall make the order... Board by accountants and firms. An accountant or accounting firm that provides audit services to a...

12 CFR 263.403 - Automatic removal, suspension, and debarment.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Accountants From Performing Audit Services § 263.403 Automatic removal, suspension, and debarment. (a) An independent public accountant or accounting firm may not perform audit services for banking organizations if the accountant or firm: (1) Is subject to a final order of removal, suspension, or debarment (other...

12 CFR 263.404 - Notice of removal, suspension, or debarment.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Accountants From Performing Audit Services § 263.404 Notice of removal, suspension, or debarment. (a) Notice... independent public accountant or accounting firm from providing audit services, the Board shall make the order... Board by accountants and firms. An accountant or accounting firm that provides audit services to a...

12 CFR 263.404 - Notice of removal, suspension, or debarment.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Accountants From Performing Audit Services § 263.404 Notice of removal, suspension, or debarment. (a) Notice... independent public accountant or accounting firm from providing audit services, the Board shall make the order... Board by accountants and firms. An accountant or accounting firm that provides audit services to a...

12 CFR 263.13 - Change of time limits.

Code of Federal Regulations, 2010 CFR

2010-01-01

... RULES OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.13 Change of time limits. Except as otherwise provided by law, the administrative law judge may, for good cause shown, extend the time limits prescribed by the Uniform Rules or by any notice or order issued in the proceedings. After...

26 CFR 1.263A-3 - Rules relating to property acquired for resale.

Code of Federal Regulations, 2013 CFR

2013-04-01

..., attributable to transactions occurring between the two corporations. (c) Purchasing, handling, and storage... years (test period) do not exceed $10,000,000. However, taxpayers that acquire real property for resale... section 263A(b)(2)(B). (i) Test period for new taxpayers. For purposes of applying this exception, if a...

26 CFR 1.263A-3 - Rules relating to property acquired for resale.

Code of Federal Regulations, 2014 CFR

2014-04-01

..., attributable to transactions occurring between the two corporations. (c) Purchasing, handling, and storage... years (test period) do not exceed $10,000,000. However, taxpayers that acquire real property for resale... section 263A(b)(2)(B). (i) Test period for new taxpayers. For purposes of applying this exception, if a...

26 CFR 1.263A-3 - Rules relating to property acquired for resale.

Code of Federal Regulations, 2011 CFR

2011-04-01

..., attributable to transactions occurring between the two corporations. (c) Purchasing, handling, and storage... years (test period) do not exceed $10,000,000. However, taxpayers that acquire real property for resale... section 263A(b)(2)(B). (i) Test period for new taxpayers. For purposes of applying this exception, if a...

26 CFR 1.263A-3 - Rules relating to property acquired for resale.

Code of Federal Regulations, 2012 CFR

2012-04-01

..., attributable to transactions occurring between the two corporations. (c) Purchasing, handling, and storage... years (test period) do not exceed $10,000,000. However, taxpayers that acquire real property for resale... section 263A(b)(2)(B). (i) Test period for new taxpayers. For purposes of applying this exception, if a...

12 CFR 263.62 - Relevant considerations for assessment of civil penalty.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Relevant considerations for assessment of civil... Collection of Civil Money Penalties § 263.62 Relevant considerations for assessment of civil penalty. In... the penalty with respect to the financial resources and good faith of the person charged, the gravity...

45 CFR 263.3 - When do child care expenditures count?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 2 2013-10-01 2012-10-01 true When do child care expenditures count? 263.3... do child care expenditures count? (a) State funds expended to meet the requirements of the CCDF... amounts), or any other Federal child care program, may also count as basic MOE expenditures. The limit...

45 CFR 263.3 - When do child care expenditures count?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 2 2010-10-01 2010-10-01 false When do child care expenditures count? 263.3... do child care expenditures count? (a) State funds expended to meet the requirements of the CCDF... amounts), or any other Federal child care program, may also count as basic MOE expenditures. The limit...

45 CFR 263.3 - When do child care expenditures count?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 2 2011-10-01 2011-10-01 false When do child care expenditures count? 263.3... do child care expenditures count? (a) State funds expended to meet the requirements of the CCDF... amounts), or any other Federal child care program, may also count as basic MOE expenditures. The limit...

45 CFR 263.3 - When do child care expenditures count?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 2 2014-10-01 2012-10-01 true When do child care expenditures count? 263.3... do child care expenditures count? (a) State funds expended to meet the requirements of the CCDF... amounts), or any other Federal child care program, may also count as basic MOE expenditures. The limit...

45 CFR 263.3 - When do child care expenditures count?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 2 2012-10-01 2012-10-01 false When do child care expenditures count? 263.3... do child care expenditures count? (a) State funds expended to meet the requirements of the CCDF... amounts), or any other Federal child care program, may also count as basic MOE expenditures. The limit...

7 CFR 1951.263 - Graduation of non-Farm Credit programs borrowers.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 14 2012-01-01 2012-01-01 false Graduation of non-Farm Credit programs borrowers... Credit Needs and Graduation of Borrowers § 1951.263 Graduation of non-Farm Credit programs borrowers. (a... required for refinancing, or when a borrower is a public body and must issue bonds to accomplish graduation...

7 CFR 1951.263 - Graduation of non-Farm Credit programs borrowers.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 14 2013-01-01 2013-01-01 false Graduation of non-Farm Credit programs borrowers... Credit Needs and Graduation of Borrowers § 1951.263 Graduation of non-Farm Credit programs borrowers. (a... required for refinancing, or when a borrower is a public body and must issue bonds to accomplish graduation...

7 CFR 1951.263 - Graduation of non-Farm Credit programs borrowers.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 14 2011-01-01 2011-01-01 false Graduation of non-Farm Credit programs borrowers... Credit Needs and Graduation of Borrowers § 1951.263 Graduation of non-Farm Credit programs borrowers. (a... required for refinancing, or when a borrower is a public body and must issue bonds to accomplish graduation...

7 CFR 1951.263 - Graduation of non-Farm Credit programs borrowers.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 14 2014-01-01 2014-01-01 false Graduation of non-Farm Credit programs borrowers... Credit Needs and Graduation of Borrowers § 1951.263 Graduation of non-Farm Credit programs borrowers. (a... required for refinancing, or when a borrower is a public body and must issue bonds to accomplish graduation...

26 CFR 1.263A-13 - Oil and gas activities.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 263A(g)) of oil or gas property. For this purpose, oil or gas property consists of each separate... described in section 614(b) (section 614 property). Thus, an oil or gas property is designated property... rules of this paragraph (c) are consistently applied for all the taxpayer's oil or gas properties for...

45 CFR 263.2 - What kinds of State expenditures count toward meeting a State's basic MOE expenditure requirement?

Code of Federal Regulations, 2013 CFR

2013-10-01

... Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN...) Cash assistance, including the State's share of the assigned child support collection that is... payment; (2) Child care assistance (see § 263.3); (3) Education activities designed to increase self...

45 CFR 263.2 - What kinds of State expenditures count toward meeting a State's basic MOE expenditure requirement?

Code of Federal Regulations, 2014 CFR

2014-10-01

... Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN...) Cash assistance, including the State's share of the assigned child support collection that is... payment; (2) Child care assistance (see § 263.3); (3) Education activities designed to increase self...

45 CFR 263.2 - What kinds of State expenditures count toward meeting a State's basic MOE expenditure requirement?

Code of Federal Regulations, 2010 CFR

2010-10-01

... Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN...) Cash assistance, including the State's share of the assigned child support collection that is... payment; (2) Child care assistance (see § 263.3); (3) Education activities designed to increase self...

45 CFR 263.2 - What kinds of State expenditures count toward meeting a State's basic MOE expenditure requirement?

Code of Federal Regulations, 2011 CFR

2011-10-01

... Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN...) Cash assistance, including the State's share of the assigned child support collection that is... payment; (2) Child care assistance (see § 263.3); (3) Education activities designed to increase self...

45 CFR 263.2 - What kinds of State expenditures count toward meeting a State's basic MOE expenditure requirement?

Code of Federal Regulations, 2012 CFR

2012-10-01

... Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN...) Cash assistance, including the State's share of the assigned child support collection that is... payment; (2) Child care assistance (see § 263.3); (3) Education activities designed to increase self...

33 CFR 263.25 - Authority for emergency streambank and shoreline protection of public works and nonprofit public...

Code of Federal Regulations, 2010 CFR

2010-07-01

... protection works to prevent damage to highways, bridge approaches, public works, churches, hospitals, schools... streambank and shoreline protection of public works and nonprofit public services (Section 14). 263.25... and shoreline protection of public works and nonprofit public services (Section 14). (a) Legislative...

50 CFR 648.263 - Red crab possession and landing restrictions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Red crab possession and landing... Management Measures for the Atlantic Deep-Sea Red Crab Fishery § 648.263 Red crab possession and landing restrictions. (a) Vessels issued limited access red crab permits. (1) [Reserved] (2) Conversion to whole crab...

12 CFR 263.22 - Consolidation and severance of actions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Consolidation and severance of actions. (a) Consolidation. (1) On the motion of any party, or on the administrative law judge's own motion, the administrative law judge may consolidate, for some or all purposes... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Consolidation and severance of actions. 263.22...

34 CFR 263.21 - What priority is given to certain projects and applicants?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 34 Education 1 2014-07-01 2014-07-01 false What priority is given to certain projects and... PROGRAMS Demonstration Grants for Indian Children Program § 263.21 What priority is given to certain... subject matters, including math and science, to enable Indian students to successfully transition to...

33 CFR 263.25 - Authority for emergency streambank and shoreline protection of public works and nonprofit public...

Code of Federal Regulations, 2014 CFR

2014-07-01

... Environmental Quality objectives. (c) Legislative interpretations. (1) “Public Works” are considered to be those... streambank and shoreline protection of public works and nonprofit public services (Section 14). 263.25... and shoreline protection of public works and nonprofit public services (Section 14). (a) Legislative...

Effects of oral Lactobacillus administration on antioxidant activities and CD4+CD25+forkhead box P3 (FoxP3)+ T cells in NZB/W F1 mice.

PubMed

Tzang, Bor-Show; Liu, Chung-Hsien; Hsu, Kuo-Ching; Chen, Yi-Hsing; Huang, Chih-Yang; Hsu, Tsai-Ching

2017-09-01

Systemic lupus erythematosus (SLE) is an autoimmune disease that is characterised by a dysregulation of the immune system, which causes inflammation responses, excessive oxidative stress and a reduction in the number of cluster of differentiation (CD)4+CD25+forkhead box P3 (FoxP3)+ T cells. Supplementation with certain Lactobacillus strains has been suggested to be beneficial in the comprehensive treatment of SLE. However, little is known about the effect and mechanism of certain Lactobacillus strains on SLE. To investigate the effects of Lactobacillus on SLE, NZB/W F1 mice were orally gavaged with Lactobacillus paracasei GMNL-32 (GMNL-32), Lactobacillus reuteri GMNL-89 (GMNL-89) and L. reuteri GMNL-263 (GMNL-263). Supplementation with GMNL-32, GMNL-89 and GMNL-263 significantly increased antioxidant activity, reduced IL-6 and TNF-α levels and significantly decreased the toll-like receptors/myeloid differentiation primary response gene 88 signalling in NZB/W F1 mice. Notably, supplementation with GMNL-263, but not GMNL-32 and GMNL-89, in NZB/W F1 mice significantly increased the differentiation of CD4+CD25+FoxP3+ T cells. These findings reveal beneficial effects of GMNL-32, GMNL-89 and GMNL-263 on NZB/W F1 mice and suggest that these specific Lactobacillus strains can be used as part of a comprehensive treatment of SLE patients.

Terrain Park Injuries

PubMed Central

Moffat, Craig; McIntosh, Scott; Bringhurst, Jade; Danenhauer, Karen; Gilmore, Nathan; Hopkins, Christy L.

2009-01-01

Background: This study examined demographics, injury pattern, and hospital outcome in patients injured in winter resort terrain parks. Methods: The study included patients ≥12 years of age who presented to a regional trauma center with an acute injury sustained at a winter resort. Emergency department (ED) research assistants collected patient injury and helmet use information using a prospectively designed questionnaire. ED and hospital data were obtained from trauma registry and hospital records. Results: Seventy-two patients were injured in a terrain park, and 263 patients were injured on non-terrain park slopes. Patients injured in terrain parks were more likely to be male [68/72 (94%) vs. 176/263 (67%), p<0.0001], younger in age [23 ± 7 vs. 36 ± 17, p<0.0001], live locally [47/72 (65%) vs. 124/263 (47%), p=0.006], use a snowboard [50/72 (69%) vs. 91/263 (35%), p<0.0001], hold a season pass [46/66 (70%) vs. 98/253 (39%), p<0.0001], and sustain an upper extremity injury [29/72 (40%) vs. 52/263 (20%), p<0.001] when compared to patients injured on non-terrain park slopes. There were no differences between the groups in terms of EMS transport to hospital, helmet use, admission rate, hospital length of stay, and patients requiring specialty consultation in the ED. Conclusions: Patients injured in terrain parks represent a unique demographic within winter resort patrons. Injury severity appears to be similar to those patients injured on non-terrain park slopes. PMID:20046245

Highly Efficient Luminescent Metal-Organic Framework for the Simultaneous Detection and Removal of Heavy Metals from Water.

PubMed

Rudd, Nathan D; Wang, Hao; Fuentes-Fernandez, Erika M A; Teat, Simon J; Chen, Feng; Hall, Gene; Chabal, Yves J; Li, Jing

2016-11-09

We have designed and synthesized an isoreticular series of luminescent metal-organic frameworks (LMOFs) by incorporating a strongly emissive molecular fluorophore and functionally diverse colinkers into Zn-based structures. The three-dimensional porous networks of LMOF-261, -262, and -263 represent a unique/new type of nets, classified as a 2-nodal, (4,4)-c net (mot-e type) with 4-fold, class IIIa interpenetration. All compounds crystallize in a body-centered tetragonal crystal system (space group I4 1 /a). A systematic study has been implemented to analyze their interactions with heavy metals. LMOF-263 exhibits impressive water stability, high porosity, and strong luminescence, making it an excellent candidate as a fluorescent chemical sensor and adsorbent for aqueous contaminants. It is extremely responsive to toxic heavy metals at a parts per billion level (3.3 ppb Hg 2+ , 19.7 ppb Pb 2+ ) and demonstrates high selectivity for heavy metals over light metals, with detection ratios of 167.4 and 209.5 for Hg 2+ /Ca 2+ and Hg 2+ /Mg 2+ , respectively. Mixed-metal adsorption experiments also show that LMOF-263 selectively adsorbs Hg 2+ over other heavy metal ions in addition to light metals. The Pb 2+ K SV value for LMOF-263 (55,017 M -1 ) is the highest among LMOFs reported to date, and the Hg 2+ K SV value is the second highest (459,446 M -1 ). LMOF-263 exhibits a maximum adsorption capacity of 380 mg Hg 2+ /g. The Hg 2+ adsorption process follows pseudo-second-order kinetics, removing 99.1% of the metal within 30 min. An in situ XPS study provides insight to help understand the interaction mechanism between Hg 2+ and LMOF-263. No other MOFs have demonstrated such a high performance in both the detection and the capture of Hg 2+ from aqueous solution.

p62/sequestosome-1 up-regulation promotes ABT-263-induced caspase-8 aggregation/activation on the autophagosome.

PubMed

Huang, Shengbing; Okamoto, Koichi; Yu, Chunrong; Sinicrope, Frank A

2013-11-22

Autophagy and apoptosis regulate cancer cell viability in response to cytotoxic stress; however, their functional relationship remains unclear. p62/sequestosome 1 is a multifunctional protein and a signaling hub that shuttles ubiquitinated proteins to the lysosome during autophagy. Autophagy inhibition up-regulates p62, and prior data suggest that p62 may mediate apoptosis. Here, we demonstrate that p62 can regulate a caspase-8-dependent apoptosis in response to the BH3 mimetic agent, ABT-263. Up-regulation of p62 was shown to enhance ABT-263-induced caspase-8 activation that was Bax-dependent and resulted from mitochondrial amplification. Dependence upon caspase-8 was confirmed using caspase-8-deficient cells and by caspase-8 siRNA. Ectopic wild-type p62, but not p62 mutants with loss of ability to promote apoptosis, was shown to co-localize with caspase-8 and to promote its self-aggregation in ABT-263-treated cells, shown using a bimolecular fluorescence complementation assay. Endogenous p62 co-localized with caspase-8 in the presence of ABT-263 plus an autophagy inhibitor. Caspase-8 was shown to interact and co-localize with the autophagosome marker, LC3II. Knockdown of p62 attenuated binding between caspase-8 and LC3II, whereas p62 overexpression enhanced the co-localization of caspase-8 aggregates with LC3. LC3 knockdown did not affect interaction between caspase-8 and p62, suggesting that p62 may facilitate caspase-8 translocation to the autophagosomal membrane. A direct activator of caspase-8, i.e., TRAIL, alone or combined with ABT-263, induced caspase-8 aggregation and co-localization with p62 that was associated with a synergistic drug interaction. Together, these results demonstrate that up-regulation of p62 can mediate apoptosis via caspase-8 in the setting of autophagy inhibition.

p62/Sequestosome-1 Up-regulation Promotes ABT-263-induced Caspase-8 Aggregation/Activation on the Autophagosome*

PubMed Central

Huang, Shengbing; Okamoto, Koichi; Yu, Chunrong; Sinicrope, Frank A.

2013-01-01

Autophagy and apoptosis regulate cancer cell viability in response to cytotoxic stress; however, their functional relationship remains unclear. p62/sequestosome 1 is a multifunctional protein and a signaling hub that shuttles ubiquitinated proteins to the lysosome during autophagy. Autophagy inhibition up-regulates p62, and prior data suggest that p62 may mediate apoptosis. Here, we demonstrate that p62 can regulate a caspase-8-dependent apoptosis in response to the BH3 mimetic agent, ABT-263. Up-regulation of p62 was shown to enhance ABT-263-induced caspase-8 activation that was Bax-dependent and resulted from mitochondrial amplification. Dependence upon caspase-8 was confirmed using caspase-8-deficient cells and by caspase-8 siRNA. Ectopic wild-type p62, but not p62 mutants with loss of ability to promote apoptosis, was shown to co-localize with caspase-8 and to promote its self-aggregation in ABT-263-treated cells, shown using a bimolecular fluorescence complementation assay. Endogenous p62 co-localized with caspase-8 in the presence of ABT-263 plus an autophagy inhibitor. Caspase-8 was shown to interact and co-localize with the autophagosome marker, LC3II. Knockdown of p62 attenuated binding between caspase-8 and LC3II, whereas p62 overexpression enhanced the co-localization of caspase-8 aggregates with LC3. LC3 knockdown did not affect interaction between caspase-8 and p62, suggesting that p62 may facilitate caspase-8 translocation to the autophagosomal membrane. A direct activator of caspase-8, i.e., TRAIL, alone or combined with ABT-263, induced caspase-8 aggregation and co-localization with p62 that was associated with a synergistic drug interaction. Together, these results demonstrate that up-regulation of p62 can mediate apoptosis via caspase-8 in the setting of autophagy inhibition. PMID:24121507

33 CFR Appendix B to Part 263 - Application of Multiobjective Planning Framework to Continuing Authorities Program

Code of Federal Regulations, 2014 CFR

2014-07-01

... Authorities Program 1. General. The planning process described in the ER 1105-2-200 series of regulations... at the same time keeping the requirements for information and analyses consistent with the scope of the study, solutions recommended, and the Program completion-time objectives outlined in § 263.18 of...

33 CFR Appendix B to Part 263 - Application of Multiobjective Planning Framework to Continuing Authorities Program

Code of Federal Regulations, 2012 CFR

2012-07-01

... Authorities Program 1. General. The planning process described in the ER 1105-2-200 series of regulations... at the same time keeping the requirements for information and analyses consistent with the scope of the study, solutions recommended, and the Program completion-time objectives outlined in § 263.18 of...

33 CFR Appendix B to Part 263 - Application of Multiobjective Planning Framework to Continuing Authorities Program

Code of Federal Regulations, 2011 CFR

2011-07-01

... Authorities Program 1. General. The planning process described in the ER 1105-2-200 series of regulations... at the same time keeping the requirements for information and analyses consistent with the scope of the study, solutions recommended, and the Program completion-time objectives outlined in § 263.18 of...

33 CFR Appendix B to Part 263 - Application of Multiobjective Planning Framework to Continuing Authorities Program

Code of Federal Regulations, 2010 CFR

2010-07-01

... Authorities Program 1. General. The planning process described in the ER 1105-2-200 series of regulations... at the same time keeping the requirements for information and analyses consistent with the scope of the study, solutions recommended, and the Program completion-time objectives outlined in § 263.18 of...

33 CFR Appendix B to Part 263 - Application of Multiobjective Planning Framework to Continuing Authorities Program

Code of Federal Regulations, 2013 CFR

2013-07-01

... Authorities Program 1. General. The planning process described in the ER 1105-2-200 series of regulations... at the same time keeping the requirements for information and analyses consistent with the scope of the study, solutions recommended, and the Program completion-time objectives outlined in § 263.18 of...

77 FR 54930 - Chartis Global Services, Inc., a Subsidiary of Chartis, Inc., Regional Processing Organization...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-06

... Organization, Regional Service Center, Dallas, TX; Amended Certification Regarding Eligibility To Apply for... Processing Organization and that workers and former workers at an affiliated facility in Dallas, Texas... location in Dallas, Texas (TA-W- 81,263A). The amended notice applicable to TA-W-81,263 is hereby issued as...

26 CFR 1.263(a)-2T - Amounts paid to acquire or produce tangible property (temporary).

Code of Federal Regulations, 2012 CFR

2012-04-01

..., see section 263A requiring taxpayers to capitalize the direct and indirect costs of producing property... direct and allocable indirect costs (including otherwise deductible costs) to be capitalized to property... company to move storage tanks from Y's plant to X's plant. Under paragraph (f)(2)(ii)(A) of this section...

26 CFR 1.263(a)-2T - Amounts paid to acquire or produce tangible property (temporary).

Code of Federal Regulations, 2013 CFR

2013-04-01

..., see section 263A requiring taxpayers to capitalize the direct and indirect costs of producing property... direct and allocable indirect costs (including otherwise deductible costs) to be capitalized to property... company to move storage tanks from Y's plant to X's plant. Under paragraph (f)(2)(ii)(A) of this section...

26 CFR 1.263A-7 - Changing a method of accounting under section 263A.

Code of Federal Regulations, 2010 CFR

2010-04-01

... extrapolation, rather than based on the facts and circumstances of a particular year's data. All three methods... analyze the production and resale data for that particular year and apply the rules and principles of... books and records, actual financial and accounting data which is required to apply the capitalization...

26 CFR 1.263A-7 - Changing a method of accounting under section 263A.

Code of Federal Regulations, 2011 CFR

2011-04-01

... extrapolation, rather than based on the facts and circumstances of a particular year's data. All three methods... analyze the production and resale data for that particular year and apply the rules and principles of... books and records, actual financial and accounting data which is required to apply the capitalization...

GCK-MODY diabetes associated with protein misfolding, cellular self-association and degradation.

PubMed

Negahdar, Maria; Aukrust, Ingvild; Johansson, Bente B; Molnes, Janne; Molven, Anders; Matschinsky, Franz M; Søvik, Oddmund; Kulkarni, Rohit N; Flatmark, Torgeir; Njølstad, Pål Rasmus; Bjørkhaug, Lise

2012-11-01

GCK-MODY, dominantly inherited mild fasting hyperglycemia, has been associated with >600 different mutations in the glucokinase (GK)-encoding gene (GCK). When expressed as recombinant pancreatic proteins, some mutations result in enzymes with normal/near-normal catalytic properties. The molecular mechanism(s) of GCK-MODY due to these mutations has remained elusive. Here, we aimed to explore the molecular mechanisms for two such catalytically 'normal' GCK mutations (S263P and G264S) in the F260-L270 loop of GK. When stably overexpressed in HEK293 cells and MIN6 β-cells, the S263P- and G264S-encoded mutations generated misfolded proteins with an increased rate of degradation (S263P>G264S) by the protein quality control machinery, and a propensity to self-associate (G264S>S263P) and form dimers (SDS resistant) and aggregates (partly Triton X-100 insoluble), as determined by pulse-chase experiments and subcellular fractionation. Thus, the GCK-MODY mutations S263P and G264S lead to protein misfolding causing destabilization, cellular dimerization/aggregation and enhanced rate of degradation. In silico predicted conformational changes of the F260-L270 loop structure are considered to mediate the dimerization of both mutant proteins by a domain swapping mechanism. Thus, similar properties may represent the molecular mechanisms for additional unexplained GCK-MODY mutations, and may also contribute to the disease mechanism in other previously characterized GCK-MODY inactivating mutations. Copyright © 2012 Elsevier B.V. All rights reserved.

Prenatal diagnosis and molecular cytogenetic characterization of rec(10)dup(10p)inv(10)(p11.2q26.3) in a fetus associated with paternal pericentric inversion.

PubMed

Chen, Chih-Ping; Ko, Tsang-Ming; Su, Yi-Ning; Wang, Liang-Kai; Chern, Schu-Rern; Wu, Peih-Shan; Chen, Yen-Ni; Chen, Shin-Wen; Ko, Kevin; Lee, Chen-Chi; Chen, Li-Feng; Yang, Chien-Wen; Wang, Wayseen

2016-10-01

We present prenatal diagnosis and molecular cytogenetic characterization of a recombinant chromosome 10 in a fetus associated with a paternal pericentric inversion. A 35-year-old woman underwent amniocentesis at 18 weeks of gestation because of an advanced maternal age. Amniocentesis revealed a karyotype of 46,XY,der(10)del(10) (q26.3)dup(10)(p11.2p15). She underwent repeat amniocentesis at 21 weeks of gestation and array comparative genomic hybridization revealed a 31.65-Mb duplication of chromosome 10p15.3-p11.22 and a 3.07-Mb deletion of chromosome 10q26.3. Prenatal ultrasound findings were unremarkable. She was referred for genetic counseling and cytogenetic analysis revealed a karyotype of 46,XY,inv(10)(p11.2q26.3) in the father and a karyotype of 46,XX in the mother. The pregnancy was subsequently terminated, and a fetus was delivered with prominent facial dysmorphism. Postnatal cytogenetic analysis of the placenta revealed a karyotype of 46,XY, rec(10)dup(10p)inv(10)(p11.2q26.3). Fluorescence in situ hybridization analysis revealed a duplication of terminal 10p and a deletion of terminal 10q in the recombinant chromosome 10. Array comparative genomic hybridization analysis of the cord blood and umbilical cord confirmed the prenatal diagnosis. Prenatal diagnosis of a recombinant chromosome because of an advanced maternal age should alert the possibility of a paternal pericentric inversion. Copyright © 2016. Published by Elsevier B.V.

7 CFR 868.261 - Grade and grade requirements for the classes of brown rice for processing. (See also § 868.263.)

Code of Federal Regulations, 2012 CFR

2012-01-01

... rice for processing. (See also § 868.263.) 868.261 Section 868.261 Agriculture Regulations of the... COMMODITIES United States Standards for Brown Rice for Processing Principles Governing Application of Standards § 868.261 Grade and grade requirements for the classes of brown rice for processing. (See also...

7 CFR 868.261 - Grade and grade requirements for the classes of brown rice for processing. (See also § 868.263.)

Code of Federal Regulations, 2010 CFR

2010-01-01

... rice for processing. (See also § 868.263.) 868.261 Section 868.261 Agriculture Regulations of the... COMMODITIES United States Standards for Brown Rice for Processing Principles Governing Application of Standards § 868.261 Grade and grade requirements for the classes of brown rice for processing. (See also...

7 CFR 868.261 - Grade and grade requirements for the classes of brown rice for processing. (See also § 868.263.)

Code of Federal Regulations, 2013 CFR

2013-01-01

... rice for processing. (See also § 868.263.) 868.261 Section 868.261 Agriculture Regulations of the... COMMODITIES United States Standards for Brown Rice for Processing Principles Governing Application of Standards § 868.261 Grade and grade requirements for the classes of brown rice for processing. (See also...

7 CFR 868.261 - Grade and grade requirements for the classes of brown rice for processing. (See also § 868.263.)

Code of Federal Regulations, 2014 CFR

2014-01-01

... rice for processing. (See also § 868.263.) 868.261 Section 868.261 Agriculture Regulations of the... COMMODITIES United States Standards for Brown Rice for Processing Principles Governing Application of Standards § 868.261 Grade and grade requirements for the classes of brown rice for processing. (See also...

7 CFR 868.261 - Grade and grade requirements for the classes of brown rice for processing. (See also § 868.263.)

Code of Federal Regulations, 2011 CFR

2011-01-01

... rice for processing. (See also § 868.263.) 868.261 Section 868.261 Agriculture Regulations of the... COMMODITIES United States Standards for Brown Rice for Processing Principles Governing Application of Standards § 868.261 Grade and grade requirements for the classes of brown rice for processing. (See also...

Corrigendum to ;Wounded quarks and diquarks in heavy ion collisions; [Phys. Lett. B 649 (2007) 263

NASA Astrophysics Data System (ADS)

Bialas, A.; Bzdak, A.

2017-10-01

Numerical result in Eq. (15), σqq /σNN = 1.147 - 1.148, as given in [Phys. Lett. B 649 (2007) 263] should be corrected to σqq /σNN = 0.147 - 0.148. The conclusions of the original paper remain unchanged. We thank Partha Pratim Bhaduri for pointing out this typo.

25 CFR 26.3 - What is the purpose of the Job Placement and Training Program?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false What is the purpose of the Job Placement and Training... PLACEMENT AND TRAINING PROGRAM General Applicability § 26.3 What is the purpose of the Job Placement and Training Program? The purpose of the Job Placement and Training Program is to assist eligible applicants to...

Composite Laminate With Coefficient of Thermal Expansion Matching D263 Glass

NASA Technical Reports Server (NTRS)

Robinson, David; Rodini, Benjamin

2012-01-01

The International X-ray Observatory project seeks to make an X-ray telescope assembly with 14,000 flexible glass segments. The glass used is commercially available SCHOTT D263 glass. Thermal expansion causes the mirror to distort out of alignment. A housing material is needed that has a matching coefficient of thermal expansion (CTE) so that when temperatures change in the X-ray mirror assembly, the glass and housing pieces expand equally, thus reducing or eliminating distortion. Desirable characteristics of this material include a high stiffness/weight ratio, and low density. Some metal alloys show promise in matching the CTE of D263 glass, but their density is high compared to aluminum, and their stiffness/weight ratio is not favorable. A laminate made from carbon fiber reinforced plastic (CFRP) should provide more favorable characteristics, but there has not been any made with the CTE matching D263 Glass. It is common to create CFRP laminates of various CTEs by stacking layers of prepreg material at various angles. However, the CTE of D263 glass is 6.3 ppm/ C at 20 C, which is quite high, and actually unachievable solely with carbon fiber and resin. A composite laminate has been developed that has a coefficient of thermal expansion identical to that of SCHOTT D263 glass. The laminate is made of a combination of T300 carbon fiber, Eglass, and RS3C resin. The laminate has 50% uni-T300 plies and 50% uni-E-glass plies, with each fiber-layer type laid up in a quasi-isotropic laminate for a total of 16 plies. The fiber volume (percent of fiber compared to the resin) controls the CTE to a great extent. Tests have confirmed that a fiber volume around 48% gives a CTE of 6.3 ppm/ C. This is a fairly simple composite laminate, following well established industry procedures. The unique feature of this laminate is a somewhat unusual combination of carbon fiber with E-glass (fiberglass). The advantage is that the resulting CTE comes out to 6.3 ppm/ C at 20 C, which matches D263 glass. The trick with this laminate is to establish the proper fiber volume to get the desired CTE. Laminates were made with several different fiber volumes and coupons were tested to establish the relationship between fiber volume and CTE. Testing proved that fiber volume should be about 48%.

40 CFR 421.263 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... Ammonia (as N) 599.900 263.700 (b) Raw material granulation. BAT Limitations for the Secondary Precious....051 Zinc 0.653 0.269 Combined metals 0.192 Palladium 0.064 Platinum 0.064 Ammonia (as N) 85.310 37.500... Combined metals 0.300 Ammonia (as N) 133.300 58.600 (d) Spent cyanide stripping solutions. BAT Limitations...

40 CFR 421.263 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... Ammonia (as N) 599.900 263.700 (b) Raw material granulation. BAT Limitations for the Secondary Precious....051 Zinc 0.653 0.269 Combined metals 0.192 Palladium 0.064 Platinum 0.064 Ammonia (as N) 85.310 37.500... Combined metals 0.300 Ammonia (as N) 133.300 58.600 (d) Spent cyanide stripping solutions. BAT Limitations...

40 CFR 421.263 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... Ammonia (as N) 599.900 263.700 (b) Raw material granulation. BAT Limitations for the Secondary Precious....051 Zinc 0.653 0.269 Combined metals 0.192 Palladium 0.064 Platinum 0.064 Ammonia (as N) 85.310 37.500... Combined metals 0.300 Ammonia (as N) 133.300 58.600 (d) Spent cyanide stripping solutions. BAT Limitations...

40 CFR 421.263 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... Ammonia (as N) 599.900 263.700 (b) Raw material granulation. BAT Limitations for the Secondary Precious....051 Zinc 0.653 0.269 Combined metals 0.192 Palladium 0.064 Platinum 0.064 Ammonia (as N) 85.310 37.500... Combined metals 0.300 Ammonia (as N) 133.300 58.600 (d) Spent cyanide stripping solutions. BAT Limitations...

26 CFR 1.263(c)-1 - Intangible drilling and development costs in the case of oil and gas wells.

Code of Federal Regulations, 2014 CFR

2014-04-01

... § 1.263(c)-1 Intangible drilling and development costs in the case of oil and gas wells. For rules relating to the option to deduct as expenses intangible drilling and development costs in the case of oil... 26 Internal Revenue 3 2014-04-01 2014-04-01 false Intangible drilling and development costs in the...

26 CFR 1.263(c)-1 - Intangible drilling and development costs in the case of oil and gas wells.

Code of Federal Regulations, 2013 CFR

2013-04-01

... § 1.263(c)-1 Intangible drilling and development costs in the case of oil and gas wells. For rules relating to the option to deduct as expenses intangible drilling and development costs in the case of oil... 26 Internal Revenue 3 2013-04-01 2013-04-01 false Intangible drilling and development costs in the...

26 CFR 1.263(c)-1 - Intangible drilling and development costs in the case of oil and gas wells.

Code of Federal Regulations, 2011 CFR

2011-04-01

... § 1.263(c)-1 Intangible drilling and development costs in the case of oil and gas wells. For rules relating to the option to deduct as expenses intangible drilling and development costs in the case of oil... 26 Internal Revenue 3 2011-04-01 2011-04-01 false Intangible drilling and development costs in the...

26 CFR 1.263(c)-1 - Intangible drilling and development costs in the case of oil and gas wells.

Code of Federal Regulations, 2012 CFR

2012-04-01

... § 1.263(c)-1 Intangible drilling and development costs in the case of oil and gas wells. For rules relating to the option to deduct as expenses intangible drilling and development costs in the case of oil... 26 Internal Revenue 3 2012-04-01 2012-04-01 false Intangible drilling and development costs in the...

Ultra-urban baseflow and stormflow concentrations and fluxes in a watershed undergoing restoration (WS263)

Treesearch

Kenneth T. Belt; William P. Stack; Richard V. Pouyat; Kimberly Burgess; Peter M. Groffman; William M. Frost; Sujay S. Kaushal; Guy Hager

2014-01-01

We discuss the results of sampling baseflow and stormwater runoff in Watershed 263, an ultra-urban catchment in west Baltimore City that is undergoing restoration aimed at both improving water quality as well as the quality of life in its neighborhoods. We focus on urban hydrology and describe the high baseflow and stormwater nutrient, metal, bacterial and other...

45 CFR 263.1 - How much State money must a State expend annually to meet the basic MOE requirement?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 2 2010-10-01 2010-10-01 false How much State money must a State expend annually... State's Maintenance of Effort? § 263.1 How much State money must a State expend annually to meet the... historic State expenditures. (2) However, if a State meets the minimum work participation rate requirements...

Constitutional 3p26.3 terminal microdeletion in an adolescent with neuroblastoma.

PubMed

Pezzolo, Annalisa; Sementa, Angela Rita; Lerone, Margherita; Morini, Martina; Ognibene, Marzia; Defferrari, Raffaella; Mazzocco, Katia; Conte, Massimo; Gigliotti, Anna Rita; Garaventa, Alberto; Pistoia, Vito; Varesio, Luigi

2017-05-04

Neuroblastoma (NB) is a common and often lethal cancer of early childhood that accounts for 10% of pediatric cancer mortality. Incidence peaks in infancy and then rapidly declines, with less than 5% of cases diagnosed in children and adolescents ≥ 10 y. There is increasing evidence that NB has unique biology and an chronic disease course in older children and adolescents, but ultimately dismal survival. We describe a rare constitutional 3p26.3 terminal microdeletion which occurred in an adolescent with NB, with apparently normal phenotype without neurocognitive defects. We evaluated the association of expression of genes involved in the microdeletion with NB patient outcomes using R2 platform. We screened NB patient's tumor cells for CHL1 protein expression using immunofluorescence. Constitutional and tumor DNA were tested by array-comparative genomic hybridization and single nucleotide-polymorphism-array analyses. Peripheral blood mononuclear cells from the patient showed a 2.54 Mb sub-microscopic constitutional terminal 3p deletion that extended to band p26.3. The microdeletion 3p disrupted the CNTN4 gene and the neighboring CNTN6 and CHL1 genes were hemizygously deleted, each of these genes encode neuronal cell adhesion molecules. Low expression of CNTN6 and CNTN4 genes did not stratify NB patients, whereas low CHL1 expression characterized 417 NB patients having worse overall survival. CHL1 protein expression on tumor cells from the patient was weaker than positive control. This is the first report of a constitutional 3p26.3 deletion in a NB patient. Since larger deletions of 3p, indicative of the presence of one or more tumor suppressor genes in this region, occur frequently in neuroblastoma, our results pave the way to the identification of one putative NB suppressor genes mapping in 3p26.3.

45 CFR 263.8 - What happens if a State fails to meet the basic MOE requirement?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 2 2010-10-01 2010-10-01 false What happens if a State fails to meet the basic... Maintenance of Effort? § 263.8 What happens if a State fails to meet the basic MOE requirement? (a) If any State fails to meet its basic MOE requirement for any fiscal year, then we will reduce dollar-for-dollar...

Non-LTE analysis of extremely helium-rich stars. The hot sdO stars LSE 153, 259 and 263

NASA Technical Reports Server (NTRS)

Husfeld, D.; Butler, K.; Heber, U.; Drilling, J. S.

1989-01-01

Results of a non-LTE fine analysis based mainly on high-resolution CASPEC spectra for three extremely helium-rich sdO stars are discussed in order to explain hydrogen deficiency in single stars. High temperature (Teff = 70,000 to 75,000 K) and a position in the log Teff - log g diagram were found close to the Eddington limit. Various abundance estimates are derived for hydrogen (upper limits only), carbon, nitrogen, and magnesium. Hydrogen is reduced to less than 10 percent by number in LSE 153 and LSE 263, and to less than 5 percent in LSE 259. The hydrogen deficiency is accompanied by nitrogen- and carbon-enrichment in LSE 153 and LSE 259 only. In LSE 263, carbon is depleted by about 1 dex. Stellar masses obtained by assuming that a core mass-luminosity relation holds for these stars, were found to be in the range 0.6-0.9 solar mass, yielding luminosities log L/L:solar = 3.7-4.5. Two of the program stars (LSE 153 and 259) appear to be possible successors of the R CrB and helium B stars, whereas the third star (LSE 263) displays a much lower carbon content in its photosphere making it an exceptional case among the known hydrogen deficient stars.

45 CFR 263.8 - What happens if a State fails to meet the basic MOE requirement?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 2 2014-10-01 2012-10-01 true What happens if a State fails to meet the basic MOE...? § 263.8 What happens if a State fails to meet the basic MOE requirement? (a) If any State fails to meet... SFAG payable to the State for the following fiscal year. (b) If a State fails to meet its basic MOE...

45 CFR 263.8 - What happens if a State fails to meet the basic MOE requirement?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 2 2013-10-01 2012-10-01 true What happens if a State fails to meet the basic MOE...? § 263.8 What happens if a State fails to meet the basic MOE requirement? (a) If any State fails to meet... SFAG payable to the State for the following fiscal year. (b) If a State fails to meet its basic MOE...

RLE (Research Laboratory of Electronics) Progress Report Number 126.

DTIC Science & Technology

1984-01-01

Loudness 184 26.3 Binaural Hearing 186 S.26.4 Hearing Aid Research 188 26.5 Discrimination of Spectral Shape 191 26.6 Tactile Perception of Speech... beating in the pulse. It is these high intensities which are responsible for large A.C. Stark shifts and ionization RLE P.R. No. 126 12 * . . . Atomic...Department of Aeronautics and Astronautics, Massachusetts Institute of Technology, 1984. 26.3 Binaural Hearing National Institutes of Health (Grant

The C-terminal domain of glyceraldehyde 3-phosphate dehydrogenase plays an important role in suppression of tRNALys3 packaging into human immunodeficiency virus type-1 particles.

PubMed

Kishimoto, Naoki; Onitsuka-Kishimoto, Ayano; Iga, Nozomi; Takamune, Nobutoki; Shoji, Shozo; Misumi, Shogo

2016-12-01

Human immunodeficiency virus type-1 (HIV-1) requires the packaging of human tRNA Lys3 as a primer for effective viral reverse transcription. Previously, we reported that glyceraldehyde 3-phosphate dehydrogenase (GAPDH) suppresses the packaging efficiency of tRNA Lys3 . Although the binding of GAPDH to Pr55 gag is important for the suppression mechanism, it remains unclear which domain of GAPDH is responsible for the interaction with Pr55 gag . In this study, we show that Asp 256 , Lys 260 , Lys 263 and Glu 267 of GAPDH are important for the suppression of tRNA Lys3 packaging. Yeast two-hybrid analysis demonstrated that the C -terminal domain of GAPDH (151-335) interacts with both the matrix region (MA; 1-132) and capsid N -terminal domain (CA-NTD; 133-282). The D256R, K263E or E267R mutation of GAPDH led to the loss of the ability to bind to wild-type (WT) MA, and the D256R/K260E double mutation of GAPDH resulted in the loss of detectable binding activity to WT CA-NTD. In contrast, R58E, Q59A or Q63A of MA, and E76R or R82E of CA-NTD abrogated the interaction with the C -terminal domain of GAPDH. Multiple-substituted GAPDH mutant (D256R/K260E/K263E/E267R) retained the oligomeric formation with WT GAPDH in HIV-1 producing cells, but the incorporation level of the hetero-oligomer was decreased in viral particles. Furthermore, the viruses produced from cells expressing the D256R/K260E/K263E/E267R mutant restored tRNA Lys3 packaging efficiency because the mutant exerted a dominant negative effect by preventing WT GAPDH from binding to MA and CA-NTD and improved the reverse transcription. These findings indicate that the amino acids Asp 256 , Lys 260 , Lys 263 and Glu 267 of GAPDH is essential for the mechanism of tRNA Lys3 -packaging suppression and the D256R/K260E/K263E/E267R mutant of GAPDH acts in a dominant negative manner to suppress tRNA Lys3 packaging.

Effects of senolytic drugs on human mesenchymal stromal cells.

PubMed

Grezella, Clara; Fernandez-Rebollo, Eduardo; Franzen, Julia; Ventura Ferreira, Mónica Sofia; Beier, Fabian; Wagner, Wolfgang

2018-04-18

Senolytic drugs are thought to target senescent cells and might thereby rejuvenate tissues. In fact, such compounds were suggested to increase health and lifespan in various murine aging models. So far, effects of senolytic drugs have not been analysed during replicative senescence of human mesenchymal stromal cells (MSCs). In this study, we tested four potentially senolytic drugs: ABT-263 (navitoclax), quercetin, nicotinamide riboside, and danazol. The effects of these compounds were analysed during long-term expansion of MSCs, until replicative senescence. Furthermore, we determined the effect on molecular markers for replicative senescence, such as senescence-associated beta-galactosidase staining (SA-β-gal), telomere attrition, and senescence-associated DNA methylation changes. Co-culture experiments of fluorescently labelled early and late passages revealed that particularly ABT-263 had a significant but moderate senolytic effect. This was in line with reduced SA-β-gal staining in senescent MSCs upon treatment with ABT-263. However, none of the drugs had significant effects on the maximum number of population doublings, telomere length, or epigenetic senescence predictions. Of the four tested drugs, only ABT-263 revealed a senolytic effect in human MSCs-and even treatment with this compound did not rejuvenate MSCs with regard to telomere length or epigenetic senescence signature. It will be important to identify more potent senolytic drugs to meet the high hopes for regenerative medicine.

Adaptive optics imaging of the MBM 12 association. Seven binaries and an edge-on disk in a quadruple system

NASA Astrophysics Data System (ADS)

Chauvin, G.; Ménard, F.; Fusco, T.; Lagrange, A.-M.; Beuzit, J.-L.; Mouillet, D.; Augereau, J.-C.

2002-11-01

We report adaptive optics (AO) observations of the young and nearby association MBM 12 obtained with the Canada-France-Hawaii Telescope. Our main observational result is the discovery of six new binary systems, LkHα 264, E 0255+2018, RX J0255.4+2005, S18, MBM 12-10, RX J0255.3+1915, and the confirmation of HD 17332, already known as a binary. We also detected a possible quadruple system. It is composed of the close binary LkHα 263 AB (separation of ~ 0.41''), of LkHα 262 located ~ 15.25'' from LkHα 263 A, and of LkHα 263 C, located ~ 4.1'' from LkHα 263 A. A preliminary study of the binary fraction suggests a binary excess in the MBM 12 association as compared to the field and IC 348. Because of the high binarity rate, previous estimations of spectral types and measurements of IR excesses for several candidate members of MBM 12 have to be revised. LkHα 263 C is a nebulous object that we interpret as a disk oriented almost perfectly edge-on and seen in scattered light. This object has already been reported by Jayawardhana et al. (\\cite{Jayawardhana2002}). Scattered light models allow us to estimate some of the structural parameters (i.e. inclination, diameter and to a lesser extent dust mass) of the circumstellar disk. We find an inclination of 89o and a outer radius for the disk, ~ 165 AU if the distance to MBM 12 is 275 pc. With the present data set, we do not attempt to re-assess the distance to MBM 12. We estimate however that the distance to the candidate member RX J0255.3+1915 is d > 175 pc. Based on data collected at the Canada-France-Hawaii Telescope. The CFHT corporation is funded by the Governments of Canada and France, and by the University of Hawaii.

Odd-Z Transactinide Compound Nucleus Reactions Including the Discovery of 260Bh

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nelson, Sarah L.

Several reactions producing odd-Z transactinide compound nuclei were studiedwith the 88-Inch Cyclotron and the Berkeley Gas-Filled Separator at the Lawrence Berkeley National Laboratory. The goal was to produce the same compound nucleus ator near the same excitation energy with similar values of angular momentum via differentnuclear reactions. In doing so, it can be determined if there is a preference in entrancechannel, because under these experimental conditions the survival portion of Swiatecki, Siwek-Wilcznska, and Wilczynski's"Fusion By Diffusion" model is nearly identical forthe two reactions. Additionally, because the same compound nucleus is produced, theexit channel is the same. Four compound nuclei were examined in this study: 258Db, 262Bh, 266Mt, and 272Rg. These nuclei were produced by using very similar heavy-ion induced-fusion reactions which differ only by one proton in the projectile or target nucleus (e.g.: 50Ti + 209Bi vs. 51V + 208Pb). Peak 1n exit channel cross sections were determined for each reaction in each pair, and three of the four pairs' cross sections were identical within statistical uncertainties. This indicates there is not an obvious preference of entrancechannel in these paired reactions. Charge equilibration immediately prior to fusionleading to a decreased fusion barrier is the likely cause of this phenomenon. In addition to this systematic study, the lightest isotope of element 107, bohrium, was discovered in the 209Bi( 52Cr,n) reaction. 260Bh was found to decay by emission of a 10.16 MeV alpha particle with a half-life of 35more » $$+19\\atop{-9}$$ ms. The cross section is 59 pb at an excitation energy of 15.0 MeV. The effect of the N = 152 shell is also seen in this isotope's alpha particle energy, the first evidence of such an effect in Bh. All reactions studied are also compared to model predictions by Swiatecki, Siwek-Wilcznska, and Wilczynski 's" Fusion By Diffusion " theory.« less

All-optical Integrated Switches Based on Azo-benzene Liquid Crystals on Silicon

DTIC Science & Technology

2011-11-01

Glass D263 SU8 Polymer Polymer NLC n̂ n̂ Refractive index @1.55 µm Materials n// = 1.689 n⊥= 1.502 n = 1.575 n = 1.516 E7 Glass D263 SU8 ...In the other case we have a nonlinear LCW based on glass substrates. It consists in a rectangular hollow realized in SU8 photoresist two glass...and discussion 5. All optical polymeric waveguide: methods, assumptions and procedure 6. All optical polymeric waveguide: results and discussion 7

On the classification of exoplanets according to Safronov number

NASA Astrophysics Data System (ADS)

Öztürk, O.; Erdem, A.

2018-02-01

We reexamine the classification of transiting exoplanets proposed by Hansen & Barman (2007) based on equilibrium temperatures and Safronov numbers. We used more sensitive data, namely, photometric and spectroscopic orbital solutions, of 263 well-known planets given in The Exoplanet Data Explorer, while Hansen & Barman (2007) used data on 18 transiting planets. Diagrams of the planet gravity vs. orbital period, planet gravity vs. equilibrium temperature, and Safronov number vs. equilibrium temperature of the 263 transiting planets show that the division of planets into two classes is indistinct.

Real-time motion-based H.263+ frame rate control

NASA Astrophysics Data System (ADS)

Song, Hwangjun; Kim, JongWon; Kuo, C.-C. Jay

1998-12-01

Most existing H.263+ rate control algorithms, e.g. the one adopted in the test model of the near-term (TMN8), focus on the macroblock layer rate control and low latency under the assumptions of with a constant frame rate and through a constant bit rate (CBR) channel. These algorithms do not accommodate the transmission bandwidth fluctuation efficiently, and the resulting video quality can be degraded. In this work, we propose a new H.263+ rate control scheme which supports the variable bit rate (VBR) channel through the adjustment of the encoding frame rate and quantization parameter. A fast algorithm for the encoding frame rate control based on the inherent motion information within a sliding window in the underlying video is developed to efficiently pursue a good tradeoff between spatial and temporal quality. The proposed rate control algorithm also takes the time-varying bandwidth characteristic of the Internet into account and is able to accommodate the change accordingly. Experimental results are provided to demonstrate the superior performance of the proposed scheme.

Optical diagnostic suite (schlieren, interferometry, and grid image refractometry) on OMEGA EP using a 10-ps, 263-nm probe beam.

PubMed

Froula, D H; Boni, R; Bedzyk, M; Craxton, R S; Ehrne, F; Ivancic, S; Jungquist, R; Shoup, M J; Theobald, W; Weiner, D; Kugland, N L; Rushford, M C

2012-10-01

A 10-ps, 263-nm (4ω) laser is being built to probe plasmas produced on the OMEGA EP [J. H. Kelly, L. J. Waxer, V. Bagnoud, I. A. Begishev, J. Bromage, B. E. Kruschwitz, T. E. Kessler, S. J. Loucks, D. N. Maywar, R. L. McCrory et al., J. Phys. IV France 133, 75-80 (2006)]. A suite of optical diagnostics (schlieren, interferometry, and grid image refractometry) has been designed to diagnose and characterize a wide variety of plasmas. Light scattered by the probe beam is collected by an f/4 catadioptric telescope and a transport system is designed to image with a near-diffraction-limited resolution (~1 - μm full width at half maximum) over a 5-mm field of view to a diagnostic table. The transport system provides a contrast greater than 1 : 10(4) with respect to all wavelengths outside of the 263 ± 2 nm measurement range.

Optical diagnostic suite (schlieren, interferometry, and grid image refractometry) on OMEGA EP using a 10-ps, 263-nm probe beama)

NASA Astrophysics Data System (ADS)

Froula, D. H.; Boni, R.; Bedzyk, M.; Craxton, R. S.; Ehrne, F.; Ivancic, S.; Jungquist, R.; Shoup, M. J.; Theobald, W.; Weiner, D.; Kugland, N. L.; Rushford, M. C.

2012-10-01

A 10-ps, 263-nm (4ω) laser is being built to probe plasmas produced on the OMEGA EP [J. H. Kelly, L. J. Waxer, V. Bagnoud, I. A. Begishev, J. Bromage, B. E. Kruschwitz, T. E. Kessler, S. J. Loucks, D. N. Maywar, R. L. McCrory et al., J. Phys. IV France 133, 75-80 (2006)], 10.1051/jp4:2006133015. A suite of optical diagnostics (schlieren, interferometry, and grid image refractometry) has been designed to diagnose and characterize a wide variety of plasmas. Light scattered by the probe beam is collected by an f/4 catadioptric telescope and a transport system is designed to image with a near-diffraction-limited resolution (˜1 - μm full width at half maximum) over a 5-mm field of view to a diagnostic table. The transport system provides a contrast greater than 1 : 104 with respect to all wavelengths outside of the 263 ± 2 nm measurement range.

Effect of Quorum Quenching Lactonase in Clinical Isolates of Pseudomonas aeruginosa and Comparison with Quorum Sensing Inhibitors

PubMed Central

Guendouze, Assia; Plener, Laure; Bzdrenga, Janek; Jacquet, Pauline; Rémy, Benjamin; Elias, Mikael; Lavigne, Jean-Philippe; Daudé, David; Chabrière, Eric

2017-01-01

Pseudomonas aeruginosa is a Gram negative pathogenic bacterium involved in many human infections including otitis, keratitis, pneumonia, and diabetic foot ulcers. P. aeruginosa uses a communication system, referred to as quorum sensing (QS), to adopt a group behavior by synchronizing the expression of certain genes. Among the regulated traits, secretion of proteases or siderophores, motility and biofilm formation are mainly involved in the pathogenicity. Many efforts have been dedicated to the development of quorum sensing inhibitors (QSI) and quorum quenching (QQ) agents to disrupt QS. QQ enzymes have been particularly considered as they may act in a catalytic way without entering the cell. Here we focus on the lactonase SsoPox which was previously investigated for its ability to degrade the signaling molecules, acyl-homoserine lactones, in particular on the engineered variant SsoPox-W263I. We highlight the potential of SsoPox-W263I to inhibit the virulence of 51 clinical P. aeruginosa isolates from diabetic foot ulcers by decreasing the secretion of two virulence factors, proteases and pyocyanin, as well as biofilm formation. We further compared the effect of SsoPox-W263I to the comprehensively described QSI, 5-fluorouracil and C-30. We found the lactonase SsoPox-W263I to be significantly more effective than the tested QSI at their respective concentration optimum and to retain its activity after immobilization steps, paving the way for future therapeutic applications. PMID:28261183

[Identification of Hydrocarbon-Oxidizing Dietzia Bacteria from Petroleum Reservoirs Based on Phenotypic Properties and Analysis of the 16S rRNA and gyrB Genes].

PubMed

Nazina, T N; Shumkova, E S; Sokolova, D Sh; Babich, T L; Zhurina, M V; Xue, Yan-Fen; Osipov, G A; Poltaraus, A B; Tourova, T P

2015-01-01

The taxonomic position of hydrocarbon-oxidizing bacterial strains 263 and 32d isolated from formation water of the Daqing petroleum reservoir (PRC) was determined by polyphasic taxonomy techniques, including analysis of the 16S rRNA and the gyrB genes. The major chemotaxonomic characteristics of both strains, including the IV type cell wall, composition of cell wall fatty acids, mycolic acids, and menaquinones, agreed with those typical of Dietzia strains. The DNA G+C content of strains 263 and 32d were 67.8 and 67.6 mol%, respectively. Phylogenetic analysis of the 16S rRNA gene of strain 32d revealed 99.7% similarity to the gene of D. maris, making it possible to identify strain 32d as belonging to this species. The 16S rRNA gene sequence of strain 263 exhibited 99.7 and 99.9% similarity to those of D. natronolimnaea and D. cercidiphylli YIM65002(T), respectively. Analysis of the gyrB genes of the subterranean isolates and of a number of Dietzia type strains confirmed classiffication of strain 32d as a D. maris strain and of strain 263, as a D. natronolimnaea strain. A conclusion was made concerning higher resolving power of phylogenetic analysis of the gyrB gene compared to the 16S rRNA gene analysis in the case of determination of the species position of Dietzia isolates.

Relationship of envelope antigens of animal influenza viruses to human A2 influenza strains isolated in the years 1957-68*

PubMed Central

Tumova, Bela; Easterday, Bernard C.

1969-01-01

This study demonstrates relationships in envelope antigens of 4 human influenza A2 strains isolated during the period 1957-68 (including A2/Hong Kong/68), 2 strains of A/Equi-2/63 and 7 avian influenza viruses isolated in Europe, North America, and the Ukraine in the years 1960-67. Antigenic relationships among the strains were determined on the basis of haemagglutination-inhibition, virus-neutralization, strain-specific complement-fixation, and neuraminidase-inhibition tests. North American avian influenza strains, Turkey/California/64, Turkey/Massachusetts/65, Turkey/Wisconsin/66, Turkey/Ontario/6828/67, and the Italian strain Duck/Italy/574/66 are antigenically related to human A2 influenza viruses by haemagglutinin and/or neuraminidase. None of these viruses is antigenically related to the A/Equi-2/63 strains, Duck/Ukraine/2/60, Duck/Ukraine/1/63 or A2/Hong Kong/68. However, A2/Hong Kong/68 has neuraminidase similar to other A2 strains from previous years. A definite relationship was shown between the haemagglutinin of A/Equi-2/63, A2/Hong Kong/68, Duck/Ukraine/2/60 and Duck/Ukraine/1/63 strains by the use of hyperimmune sera in 3 different serological tests. Related neuraminidase was demonstrated only between A/Equi-2/63 and both duck strains from the Ukraine. The significance of these findings and their interpretation with respect to the ecology of influenza viruses are discussed. PMID:5309452

VES263: an intermediate mass YSO candidate as a new eruptive variable

NASA Astrophysics Data System (ADS)

Giannini, T.; Antoniucci, S.; Lorenzetti, D.; Harutyunyan, A.; Licchelli, D.; Munari, U.

2018-05-01

In the framework of our EXor monitoring programme dubbed EXORCISM (EXOR OptiCal and Infrared Systematic Monitoring - Antoniucci et al. 2013 PPVI, Lorenzetti et al. 2007 ApJ 665, 1182; Lorenzetti et al. 2009 ApJ 693, 1056), we observed the object VES263 signalled by a recent GAIA alert (http://gsaweb.ast.cam.ac.uk/alerts/alert/Gaia18azl) as "an emission line star undergoing repeat outbursts" having detected an alerting magnitude of 11.66 wrt a historic magnitude of 12.16.

Architecture design of motion estimation for ITU-T H.263

NASA Astrophysics Data System (ADS)

Ku, Chung-Wei; Lin, Gong-Sheng; Chen, Liang-Gee; Lee, Yung-Ping

1997-01-01

Digitalized video and audio system has become the trend of the progress in multimedia, because it provides great performance in quality and feasibility of processing. However, as the huge amount of information is needed while the bandwidth is limitted, data compression plays an important role in the system. Say, for a 176 x 144 monochromic sequence with 10 frames/sec frame rate, the bandwidth is about 2Mbps. This wastes much channel resource and limits the applications. MPEG (moving picttre ezpert groip) standardizes the video codec scheme, and it performs high compression ratio while providing good quality. MPEG-i is used for the frame size about 352 x 240 and 30 frames per second, and MPEG-2 provides scalibility and can be applied on scenes with higher definition, say HDTV (high definition television). On the other hand, some applications concerns the very low bit-rate, such as videophone and video-conferencing. Because the channel bandwidth is much limitted in telephone network, a very high compression ratio must be required. ITU-T announced the H.263 video coding standards to meet the above requirements.8 According to the simulation results of TMN-5,22 it outperforms 11.263 with little overhead of complexity. Since wireless communication is the trend in the near future, low power design of the video codec is an important issue for portable visual telephone. Motion estimation is the most computation consuming parts in the whole video codec. About 60% of the computation is spent on this parts for the encoder. Several architectures were proposed for efficient processing of block matching algorithms. In this paper, in order to meet the requirements of 11.263 and the expectation of low power consumption, a modified sandwich architecture in21 is proposed. Based on the parallel processing philosophy, low power is expected and the generation of either one motion vector or four motion vectors with half-pixel accuracy is achieved concurrently. In addition, we will present our solution how to solve the other addition modes in 11.263 with the proposed architecture.

Microduplication of 3p26.3 Implicated in Cognitive Development

PubMed Central

Te Weehi, Leah; Maikoo, Raj; Mc Cormack, Adrian; Mazzaschi, Roberto; Ashton, Fern; Zhang, Liangtao; George, Alice M.; Love, Donald R.

2014-01-01

We report here a 34-month-old boy with global developmental delay referred for molecular karyotyping and fragile X studies. Molecular karyotype analysis revealed a microduplication in the 3p26.3 region involving part of the CHL1 and CNTN6 genes. Several deletions, one translocation, and one duplication have previously been described in this region of chromosome 3. The CHL1 gene has been proposed as a dosage-sensitive gene with a central role in cognitive development, and so the microduplication reported here appears to be implicated in our patient's phenotype. PMID:24778888

TIC10/ONC201 synergizes with Bcl-2/Bcl-xL inhibition in glioblastoma by suppression of Mcl-1 and its binding partners in vitro and in vivo

PubMed Central

Karpel-Massler, Georg; Bâ, Maïmouna; Shu, Chang; Halatsch, Marc-Eric; Westhoff, Mike-Andrew; Bruce, Jeffrey N.; Canoll, Peter; Siegelin, Markus D.

2015-01-01

Glioblastoma is the most frequent primary brain tumor in adults. Current therapeutic options are sparse and the prognosis of patients suffering from this disease is grim. Abundance in intratumoral heterogeneity among different deregulated signaling pathways is a hallmark of glioblastoma and likely accounts for its recurrence and resistance to treatment. Glioblastomas harbor a plethora of deregulated pathways driving tumor formation and growth. In this study, we show that TIC10/ONC201, a promising compound that is currently in planned clinical development, along with Bcl-2/Bcl-xL inhibition by ABT263 yields a strong synergistic antiproliferative effect on pediatric, adult, proneural glioblastoma and glioma stem-like cells. On the molecular level, treatment with TIC10/ONC201 results in a posttranslational decrease of the anti-apoptotic Bcl-2 family member, myeloid cell leukemia 1 (Mcl-1), through modulation of the chaperone Bag3 and the deubiquitinase Usp9X. Consistently, the combination treatment of TIC10/ONC201 and ABT263 required the presence of functional BAX and BAK to drive intrinsic apoptosis, but is surprisingly independent of the extrinsic apoptotic pathway. Moreover, the expression of Noxa protein was required for efficient apoptosis induction by TIC10/ONC201 and ABT263. Importantly, the drug combination of TIC10/ONC201 and the BH3-mimetic, ABT263, led to a regression of tumors in vivo, without any notable toxicity and side effects. Overall, TIC10/ONC201 along with Bcl-2/Bcl-xL inhibition holds significant promise as a novel potential approach for the treatment of recalcitrant tumors such as glioblastoma. PMID:26474387

TIC10/ONC201 synergizes with Bcl-2/Bcl-xL inhibition in glioblastoma by suppression of Mcl-1 and its binding partners in vitro and in vivo.

PubMed

Karpel-Massler, Georg; Bâ, Maïmouna; Shu, Chang; Halatsch, Marc-Eric; Westhoff, Mike-Andrew; Bruce, Jeffrey N; Canoll, Peter; Siegelin, Markus D

2015-11-03

Glioblastoma is the most frequent primary brain tumor in adults. Current therapeutic options are sparse and the prognosis of patients suffering from this disease is grim. Abundance in intratumoral heterogeneity among different deregulated signaling pathways is a hallmark of glioblastoma and likely accounts for its recurrence and resistance to treatment. Glioblastomas harbor a plethora of deregulated pathways driving tumor formation and growth. In this study, we show that TIC10/ONC201, a promising compound that is currently in planned clinical development, along with Bcl-2/Bcl-xL inhibition by ABT263 yields a strong synergistic antiproliferative effect on pediatric, adult, proneural glioblastoma and glioma stem-like cells. On the molecular level, treatment with TIC10/ONC201 results in a posttranslational decrease of the anti-apoptotic Bcl-2 family member, myeloid cell leukemia 1 (Mcl-1), through modulation of the chaperone Bag3 and the deubiquitinase Usp9X. Consistently, the combination treatment of TIC10/ONC201 and ABT263 required the presence of functional BAX and BAK to drive intrinsic apoptosis, but is surprisingly independent of the extrinsic apoptotic pathway. Moreover, the expression of Noxa protein was required for efficient apoptosis induction by TIC10/ONC201 and ABT263. Importantly, the drug combination of TIC10/ONC201 and the BH3-mimetic, ABT263, led to a regression of tumors in vivo, without any notable toxicity and side effects. Overall, TIC10/ONC201 along with Bcl-2/Bcl-xL inhibition holds significant promise as a novel potential approach for the treatment of recalcitrant tumors such as glioblastoma.

Insights Into Hypertrophic Cardiomyopathy Evaluation Through Follow-up of a Founder Pathogenic Variant.

PubMed

Lorca, Rebeca; Gómez, Juan; Martín, María; Cabanillas, Rubén; Calvo, Juan; León, Víctor; Pascual, Isaac; Morís, César; Coto, Eliecer; R Reguero, José Julián

2018-04-06

Hypertrophic cardiomyopathy (HCM) is the most common inherited cardiac disease. The current challenge relies on the accurate classification of the pathogenicity of the variants. Transthoracic echocardiography (TTE) is recommended at initial evaluation and cardiac magnetic resonance (CMR) imaging should also be considered. We aimed to reappraise the penetrance and clinical expression of the MYBPC3 p.G263* variant. Three hundred and eighty-four HCM probands and a control cohort of 450 individuals were studied for the main sarcomere genes by next-generation sequencing. All MYBPC3 p.G263* carriers were identified and family screening was performed. Clinical information was recorded retrospectively before 2015 and prospectively thereafter. Extra effort was invested in performing CMR in all carriers, despite TTE results. Thirteen HCM probands and none of the controls were carriers of the MYBPC3 p.G263* pathogenic variant (according to the American College of Medical Genetics and Genomics and the Association for Molecular Pathology). A total of 39 carriers were identified with family screening. Most patients with HCM were asymptomatic at the time of diagnosis and showed late-onset disease. Despite having a relatively benign course in the young, late HCM-related complications could occur. Penetrance was around 70% when evaluated by TTE and was 87.2% with TTE plus CMR. Penetrance was age-dependent, reaching 100% in carriers older than 55 years. MYBPC3 p.G263* shares with most truncating pathogenic variants in this gene a late onset, relatively benign clinical course in the young, and high penetrance. Cardiac magnetic resonance could be a useful tool to evaluate carriers despite TTE results. Copyright © 2018 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.

Somatic Mosaicism Underlies X-linked Acrogigantism (XLAG) Syndrome in Sporadic Male Subjects

PubMed Central

Daly, Adrian F.; Yuan, Bo; Fina, Frederic; Caberg, Jean-Hubert; Trivellin, Giampaolo; Rostomyan, Liliya; de Herder, Wouter W.; Naves, Luciana A.; Metzger, Daniel; Cuny, Thomas; Rabl, Wolfgang; Shah, Nalini; Jaffrain-Rea, Marie-Lise; Zatelli, Maria Chiara; Faucz, Fabio R; Castermans, Emilie; Nanni-Metellus, Isabelle; Lodish, Maya; Muhammad, Ammar; Palmeira, Leonor; Potorac, Iulia; Mantovani, Giovanna; Neggers, Sebastian J.; Klein, Marc; Barlier, Anne; Liu, Pengfei; Ouafik, L'Houcine; Bours, Vincent; Lupski, James R.; Stratakis, Constantine A.; Beckers., Albert

2016-01-01

Somatic mosaicism has been implicated as a causative mechanism in a number of genetic and genomic disorders. X-linked acrogigantism (XLAG) syndrome is a recently characterized genomic form of pediatric gigantism due to aggressive pituitary tumors that is caused by submicroscopic chromosome Xq26.3 duplications that include GPR101. We studied XLAG syndrome patients (N=18) to determine if somatic mosaicism contributed to the genomic pathophysiology. Eighteen subjects with XLAG syndrome were identified with Xq26.3 duplications using high definition array comparative genome hybridization (HD-aCGH). We noted males with XLAG had a decreased log2 ratio compared with expected values, suggesting potential mosaicism, while females showed no such decrease. As compared with familial male XLAG cases, sporadic males had more marked evidence for mosaicism, with levels of Xq26.3 duplication between 16.1-53.8%. These characteristics were replicated using a novel, personalized breakpoint-junction specific quantification droplet digital PCR (ddPCR) technique. Using a separate ddPCR technique we studied the feasibility of identifying XLAG syndrome cases in a distinct patient population of 64 unrelated subjects with acromegaly/gigantism and identified one female gigantism patient that had increased copy number variation (CNV) threshold for GPR101 that was subsequently diagnosed as having XLAG syndrome on HD-aCGH. Employing a combination of HD-aCGH and novel ddPCR approaches, we have demonstrated, for the first time, that XLAG syndrome can be caused by variable degrees of somatic mosaicism for duplications at chromosome Xq26.3. Somatic mosaicism was shown to occur in sporadic males but not in females with XLAG syndrome, although the clinical characteristics of the disease were similarly severe in both sexes. PMID:26935837

Gigantism and acromegaly due to Xq26 microduplications and GPR101 mutation.

PubMed

Trivellin, Giampaolo; Daly, Adrian F; Faucz, Fabio R; Yuan, Bo; Rostomyan, Liliya; Larco, Darwin O; Schernthaner-Reiter, Marie Helene; Szarek, Eva; Leal, Letícia F; Caberg, Jean-Hubert; Castermans, Emilie; Villa, Chiara; Dimopoulos, Aggeliki; Chittiboina, Prashant; Xekouki, Paraskevi; Shah, Nalini; Metzger, Daniel; Lysy, Philippe A; Ferrante, Emanuele; Strebkova, Natalia; Mazerkina, Nadia; Zatelli, Maria Chiara; Lodish, Maya; Horvath, Anelia; de Alexandre, Rodrigo Bertollo; Manning, Allison D; Levy, Isaac; Keil, Margaret F; Sierra, Maria de la Luz; Palmeira, Leonor; Coppieters, Wouter; Georges, Michel; Naves, Luciana A; Jamar, Mauricette; Bours, Vincent; Wu, T John; Choong, Catherine S; Bertherat, Jerome; Chanson, Philippe; Kamenický, Peter; Farrell, William E; Barlier, Anne; Quezado, Martha; Bjelobaba, Ivana; Stojilkovic, Stanko S; Wess, Jurgen; Costanzi, Stefano; Liu, Pengfei; Lupski, James R; Beckers, Albert; Stratakis, Constantine A

2014-12-18

Increased secretion of growth hormone leads to gigantism in children and acromegaly in adults; the genetic causes of gigantism and acromegaly are poorly understood. We performed clinical and genetic studies of samples obtained from 43 patients with gigantism and then sequenced an implicated gene in samples from 248 patients with acromegaly. We observed microduplication on chromosome Xq26.3 in samples from 13 patients with gigantism; of these samples, 4 were obtained from members of two unrelated kindreds, and 9 were from patients with sporadic cases. All the patients had disease onset during early childhood. Of the patients with gigantism who did not carry an Xq26.3 microduplication, none presented before the age of 5 years. Genomic characterization of the Xq26.3 region suggests that the microduplications are generated during chromosome replication and that they contain four protein-coding genes. Only one of these genes, GPR101, which encodes a G-protein-coupled receptor, was overexpressed in patients' pituitary lesions. We identified a recurrent GPR101 mutation (p.E308D) in 11 of 248 patients with acromegaly, with the mutation found mostly in tumors. When the mutation was transfected into rat GH3 cells, it led to increased release of growth hormone and proliferation of growth hormone-producing cells. We describe a pediatric disorder (which we have termed X-linked acrogigantism [X-LAG]) that is caused by an Xq26.3 genomic duplication and is characterized by early-onset gigantism resulting from an excess of growth hormone. Duplication of GPR101 probably causes X-LAG. We also found a recurrent mutation in GPR101 in some adults with acromegaly. (Funded by the Eunice Kennedy Shriver National Institute of Child Health and Human Development and others.).

Gigantism and Acromegaly Due to Xq26 Microduplications and GPR101 Mutation

PubMed Central

Trivellin, G.; Daly, A.F.; Faucz, F.R.; Yuan, B.; Rostomyan, L.; Larco, D.O.; Schernthaner-Reiter, M.H.; Szarek, E.; Leal, L.F.; Caberg, J.-H.; Castermans, E.; Villa, C.; Dimopoulos, A.; Chittiboina, P.; Xekouki, P.; Shah, N.; Metzger, D.; Lysy, P.A.; Ferrante, E.; Strebkova, N.; Mazerkina, N.; Zatelli, M.C.; Lodish, M.; Horvath, A.; de Alexandre, R. Bertollo; Manning, A.D.; Levy, I.; Keil, M.F.; de la Luz Sierra, M.; Palmeira, L.; Coppieters, W.; Georges, M.; Naves, L.A.; Jamar, M.; Bours, V.; Wu, T.J.; Choong, C.S.; Bertherat, J.; Chanson, P.; Kamenický, P.; Farrell, W.E.; Barlier, A.; Quezado, M.; Bjelobaba, I.; Stojilkovic, S.S.; Wess, J.; Costanzi, S.; Liu, P.; Lupski, J.R.; Beckers, A.; Stratakis, C.A.

2015-01-01

BACKGROUND Increased secretion of growth hormone leads to gigantism in children and acromegaly in adults; the genetic causes of gigantism and acromegaly are poorly understood. METHODS We performed clinical and genetic studies of samples obtained from 43 patients with gigantism and then sequenced an implicated gene in samples from 248 patients with acromegaly. RESULTS We observed microduplication on chromosome Xq26.3 in samples from 13 patients with gigantism; of these samples, 4 were obtained from members of two unrelated kindreds, and 9 were from patients with sporadic cases. All the patients had disease onset during early childhood. Of the patients with gigantism who did not carry an Xq26.3 microduplication, none presented before the age of 5 years. Genomic characterization of the Xq26.3 region suggests that the microduplications are generated during chromosome replication and that they contain four protein-coding genes. Only one of these genes, GPR101, which encodes a G-protein–coupled receptor, was overexpressed in patients’ pituitary lesions. We identified a recurrent GPR101 mutation (p.E308D) in 11 of 248 patients with acromegaly, with the mutation found mostly in tumors. When the mutation was transfected into rat GH3 cells, it led to increased release of growth hormone and proliferation of growth hormone–producing cells. CONCLUSIONS We describe a pediatric disorder (which we have termed X-linked acrogigantism [X-LAG]) that is caused by an Xq26.3 genomic duplication and is characterized by early-onset gigantism resulting from an excess of growth hormone. Duplication of GPR101 probably causes X-LAG. We also found a recurrent mutation in GPR101 in some adults with acromegaly. (Funded by the Eunice Kennedy Shriver National Institute of Child Health and Human Development and others.) PMID:25470569

Somatic mosaicism underlies X-linked acrogigantism syndrome in sporadic male subjects.

PubMed

Daly, Adrian F; Yuan, Bo; Fina, Frederic; Caberg, Jean-Hubert; Trivellin, Giampaolo; Rostomyan, Liliya; de Herder, Wouter W; Naves, Luciana A; Metzger, Daniel; Cuny, Thomas; Rabl, Wolfgang; Shah, Nalini; Jaffrain-Rea, Marie-Lise; Zatelli, Maria Chiara; Faucz, Fabio R; Castermans, Emilie; Nanni-Metellus, Isabelle; Lodish, Maya; Muhammad, Ammar; Palmeira, Leonor; Potorac, Iulia; Mantovani, Giovanna; Neggers, Sebastian J; Klein, Marc; Barlier, Anne; Liu, Pengfei; Ouafik, L'Houcine; Bours, Vincent; Lupski, James R; Stratakis, Constantine A; Beckers, Albert

2016-04-01

Somatic mosaicism has been implicated as a causative mechanism in a number of genetic and genomic disorders. X-linked acrogigantism (XLAG) syndrome is a recently characterized genomic form of pediatric gigantism due to aggressive pituitary tumors that is caused by submicroscopic chromosome Xq26.3 duplications that include GPR101 We studied XLAG syndrome patients (n= 18) to determine if somatic mosaicism contributed to the genomic pathophysiology. Eighteen subjects with XLAG syndrome caused by Xq26.3 duplications were identified using high-definition array comparative genomic hybridization (HD-aCGH). We noted that males with XLAG had a decreased log2ratio (LR) compared with expected values, suggesting potential mosaicism, whereas females showed no such decrease. Compared with familial male XLAG cases, sporadic males had more marked evidence for mosaicism, with levels of Xq26.3 duplication between 16.1 and 53.8%. These characteristics were replicated using a novel, personalized breakpoint junction-specific quantification droplet digital polymerase chain reaction (ddPCR) technique. Using a separate ddPCR technique, we studied the feasibility of identifying XLAG syndrome cases in a distinct patient population of 64 unrelated subjects with acromegaly/gigantism, and identified one female gigantism patient who had had increased copy number variation (CNV) threshold for GPR101 that was subsequently diagnosed as having XLAG syndrome on HD-aCGH. Employing a combination of HD-aCGH and novel ddPCR approaches, we have demonstrated, for the first time, that XLAG syndrome can be caused by variable degrees of somatic mosaicism for duplications at chromosome Xq26.3. Somatic mosaicism was shown to occur in sporadic males but not in females with XLAG syndrome, although the clinical characteristics of the disease were similarly severe in both sexes. © 2016 Society for Endocrinology.

Polarimetry diagnostic on OMEGA EP using a 10-ps, 263-nm probe beam.

PubMed

Davies, A; Haberberger, D; Boni, R; Ivancic, S; Brown, R; Froula, D H

2014-11-01

A polarimetry diagnostic was built and characterized for magnetic-field measurements in laser-plasma experiments on the OMEGA EP laser. This diagnostic was built into the existing 4ω (263-nm) probe system that employs a 10-ps laser pulse collected with an f/4 imaging system. The diagnostic measures the rotation of the probe beam's polarization. The polarimeter uses a Wollaston prism to split the probe beam into orthogonal polarization components. Spatially localized intensity variations between images indicate polarization rotation. Magnetic fields can be calculated by combining the polarimetry data with the measured plasma density profile obtained from angular filter refractometry.

The DUV Stability of Superlattice-Doped CMOS Detector Arrays

NASA Technical Reports Server (NTRS)

Hoenk, M. E.; Carver, A. G.; Jones, T.; Dickie, M.; Cheng, P.; Greer, H. F.; Nikzad, S.; Sgro, J.; Tsur, S.

2013-01-01

JPL and Alacron have recently developed a high performance, DUV camera with a superlattice doped CMOS imaging detector. Supperlattice doped detectors achieve nearly 100% internal quantum efficiency in the deep and far ultraviolet, and a single layer, Al2O3 antireflection coating enables 64% external quantum efficiency at 263nm. In lifetime tests performed at Applied Materials using 263 nm pulsed, solid state and 193 nm pulsed excimer laser, the quantum efficiency and dark current of the JPL/Alacron camera remained stable to better than 1% precision during long-term exposure to several billion laser pulses, with no measurable degradation, no blooming and no image memory at 1000 fps.

78 FR 57685 - Guidance Regarding Deduction and Capitalization of Expenditures Related to Tangible Property

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-19

...This document contains final regulations that provide guidance on the application of sections 162(a) and 263(a) of the Internal Revenue Code (Code) to amounts paid to acquire, produce, or improve tangible property. The final regulations clarify and expand the standards in the current regulations under sections 162(a) and 263(a). These final regulations replace and remove temporary regulations under sections 162(a) and 263(a) and withdraw proposed regulations that cross referenced the text of those temporary regulations. This document also contains final regulations under section 167 regarding accounting for and retirement of depreciable property and final regulations under section 168 regarding accounting for property under the Modified Accelerated Cost Recovery System (MACRS) other than general asset accounts. The final regulations will affect all taxpayers that acquire, produce, or improve tangible property. These final regulations do not finalize or remove the 2011 temporary regulations under section 168 regarding general asset accounts and disposition of property subject to section 168, which are addressed in the notice of proposed rulemaking on this subject in the Proposed Rules section in this issue of the Federal Register.

Steam Oxidation Behavior of Advanced Steels and Ni-Based Alloys at 800 °C

NASA Astrophysics Data System (ADS)

Dudziak, T.; Boroń, L.; Deodeshmukh, V.; Sobczak, J.; Sobczak, N.; Witkowska, M.; Ratuszek, W.; Chruściel, K.

2017-03-01

This publication studies the steam oxidation behavior of advanced steels (309S, 310S and HR3C) and Ni-based alloys (Haynes® 230®, alloy 263, alloy 617 and Haynes® 282®) exposed at 800 °C for 2000 h under 1 bar pressure, in a pure water steam system. The results revealed that all exposed materials showed relatively low weight gain, with no spallation of the oxide scale within the 2000 h of exposure. XRD analysis showed that Ni-based alloys developed an oxide scale consisting of four main phases: Cr2O3 (alloy 617, Haynes® 282®, alloy 263 and Haynes® 230®), MnCr2O4 (alloy 617, Haynes® 282® and Haynes® 230®), NiCr2O4 (alloy 617) and TiO2 (alloy 263, Haynes® 282®). In contrast, advanced steels showed the development of Cr2O3, MnCr2O4, Mn7SiO12, FeMn(SiO4) and SiO2 phases. The steel with the highest Cr content showed the formation of Fe3O4 and the thickest oxide scale.

Multiframe video coding for improved performance over wireless channels.

PubMed

Budagavi, M; Gibson, J D

2001-01-01

We propose and evaluate a multi-frame extension to block motion compensation (BMC) coding of videoconferencing-type video signals for wireless channels. The multi-frame BMC (MF-BMC) coder makes use of the redundancy that exists across multiple frames in typical videoconferencing sequences to achieve additional compression over that obtained by using the single frame BMC (SF-BMC) approach, such as in the base-level H.263 codec. The MF-BMC approach also has an inherent ability of overcoming some transmission errors and is thus more robust when compared to the SF-BMC approach. We model the error propagation process in MF-BMC coding as a multiple Markov chain and use Markov chain analysis to infer that the use of multiple frames in motion compensation increases robustness. The Markov chain analysis is also used to devise a simple scheme which randomizes the selection of the frame (amongst the multiple previous frames) used in BMC to achieve additional robustness. The MF-BMC coders proposed are a multi-frame extension of the base level H.263 coder and are found to be more robust than the base level H.263 coder when subjected to simulated errors commonly encountered on wireless channels.

Viral fitness cost prevents HIV-1 from evading dolutegravir drug pressure

PubMed Central

2013-01-01

Background Clinical studies have shown that integrase strand transfer inhibitors can be used to treat HIV-1 infection. Although the first-generation integrase inhibitors are susceptible to the emergence of resistance mutations that impair their efficacy in therapy, such resistance has not been identified to date in drug-naïve patients who have been treated with the second-generation inhibitor dolutegravir. During previous in vitro selection study, we identified a R263K mutation as the most common substitution to arise in the presence of dolutegravir with H51Y arising as a secondary mutation. Additional experiments reported here provide a plausible explanation for the absence of reported dolutegravir resistance among integrase inhibitor-naïve patients to date. Results We now show that H51Y in combination with R263K increases resistance to dolutegravir but is accompanied by dramatic decreases in both enzymatic activity and viral replication. Conclusions Since H51Y and R263K may define a unique resistance pathway to dolutegravir, our results are consistent with the absence of resistance mutations in antiretroviral drug-naive patients treated with this drug. PMID:23432922

The role of apoptosis repressor with a CARD domain (ARC) in the therapeutic resistance of renal cell carcinoma (RCC): the crucial role of ARC in the inhibition of extrinsic and intrinsic apoptotic signalling.

PubMed

Toth, Csaba; Funke, Sarah; Nitsche, Vanessa; Liverts, Anna; Zlachevska, Viktoriya; Gasis, Marcia; Wiek, Constanze; Hanenberg, Helmut; Mahotka, Csaba; Schirmacher, Peter; Heikaus, Sebastian

2017-05-02

Renal cell carcinomas (RCCs) display broad resistance against conventional radio- and chemotherapies, which is due at least in part to impairments in both extrinsic and intrinsic apoptotic pathways. One important anti-apoptotic factor that is strongly overexpressed in RCCs and known to inhibit both apoptotic pathways is ARC (apoptosis repressor with a CARD domain). Expression and subcellular distribution of ARC in RCC tissue samples and RCC cell lines were determined by immunohistochemistry and fluorescent immunohistochemistry, respectively. Extrinsic and intrinsic apoptosis signalling were induced by TRAIL (TNF-related apoptosis-inducing ligand), ABT-263 or topotecan. ARC knock-down was performed in clearCa-12 cells using lentiviral transduction of pGIPZ. shRNAmir constructs. Extrinsic respectively intrinsic apoptosis were induced by TRAIL (TNF-related apoptosis-inducing ligand), ABT263 or topotecan. Potential synergistic effects were tested by pre-treatment with topotecan and subsequent treatment with ABT263. Activation of different caspases and mitochondrial depolarisation (JC-1 staining) were analysed by flow cytometry. Protein expression of Bcl-2 family members and ARC in RCC cell lines was measured by Western blotting. Statistical analysis was performed by Student's t-test. Regarding the extrinsic pathway, ARC knockdown strongly enhanced TRAIL-induced apoptosis by increasing the activation level of caspase-8. Regarding the intrinsic pathway, ARC, which was only weakly expressed in the nuclei of RCCs in vivo, exerted its anti-apoptotic effect by impairing mitochondrial activation rather than inhibiting p53. Topotecan- and ABT-263-induced apoptosis was strongly enhanced following ARC knockdown in RCC cell lines. In addition, topotecan pre-treatment enhanced ABT-263-induced apoptosis and this effect was amplified in ARC-knockdown cells. Taken together, our results are the first to demonstrate the importance of ARC protein in the inhibition of both the extrinsic and intrinsic pathways of apoptosis in RCCs. In this context, ARC cooperates with anti-apoptotic Bcl-2 family members to exert its strong anti-apoptotic effects and is therefore an important factor not only in the therapeutic resistance but also in future therapy strategies (i.e., Bcl-2 inhibitors) in RCC. In sum, targeting of ARC may enhance the therapeutic response in combination therapy protocols.

Improved performance of collagen scaffolds crosslinked by Traut's reagent and Sulfo-SMCC.

PubMed

Li, Yiming; He, Qifen; Hu, Xiucheng; Liu, Yun; Cheng, Xiaohui; Li, Xiachen; Deng, Feilong

2017-05-01

Collagen scaffolds are frequently employed for applications in regenerative medicine. In previous studies, we affirmed that Traut's reagent (2-Iminothiolane hydrochloride) and Sulfo-SMCC (4-(N-Maleimidomethyl) cyclohexane-1-carboxylic acid 3-sulpho-N-hydroxysuccinimide ester sodium salt) could covalently bind growth factors on collagen scaffolds. We also observed that crosslinking formed within the collagen scaffolds with excess dosage of Sulfo-SMCC, which improved the biological performance of collagen scaffolds together with growth factors. In order to evaluate changes in capacity caused by crosslinking, Traut's reagent and adjusted different concentrations of Sulfo-SMCC (0.263, 1.315, 2.63 and 5.26 mM) were used to construct collagen scaffolds with differing extents of crosslinking in this study. The results demonstrated that resistance of collagen scaffolds to enzymatic digestion, cellularization and vascularization in vivo were enhanced by the crosslinking procedure. The cell culture studies indicated that the crosslinking procedure did not influence biocompatibility. Moreover, there were no statistical differences in the degradation rate, cellularization or vascularization among 1.315, 2.63 and 5.26 mM crosslinked groups. These results demonstrated that crosslinking collagen scaffolds with an appropriate amount of Traut's reagent and Sulfo-SMCC was an effective and safe method to modify naturally derived collagen scaffolds with notable potential uses in tissue regeneration.

Molecular analysis of functional redundancy among anti-apoptotic Bcl-2 proteins and its role in cancer cell survival.

PubMed

Eichhorn, Joshua M; Alford, Sarah E; Sakurikar, Nandini; Chambers, Timothy C

2014-04-01

Bcl-2 family proteins act as essential regulators and mediators of intrinsic apoptosis. Several lines of evidence suggest that the anti-apoptotic members of the family, including Bcl-2, Bcl-xL and Mcl-1, exhibit functional redundancy. However, the current evidence is largely indirect, and based mainly on pharmacological data using small-molecule inhibitors. In order to study compensation and redundancy of anti-apoptotic Bcl-2 proteins at the molecular level, we used a combined knockdown/overexpression strategy to essentially replace the function of one member with another. The results show that HeLa cells are strictly dependent on Mcl-1 for survival and correspondingly refractory to the Bcl-2/Bcl-xL inhibitor ABT-263, and remain resistant to ABT-263 in the context of Bcl-xL overexpression because endogenous Mcl-1 continues to provide the primary guardian role. However, if Mcl-1 is knocked down in the context of Bcl-xL overexpression, the cells become Bcl-xL-dependent and sensitive to ABT-263. We also show that Bcl-xL compensates for loss of Mcl-1 by sequestration of two key pro-apoptotic Bcl-2 family members, Bak and Bim, normally bound to Mcl-1, and that Bim is essential for cell death induced by Mcl-1 knockdown. To our knowledge, this is the first example where cell death induced by loss of Mcl-1 was rescued by the silencing of a single BH3-only Bcl-2 family member. In colon carcinoma cell lines, Bcl-xL and Mcl-1 also play compensatory roles, and Mcl-1 knockdown sensitizes cells to ABT-263. The results, obtained employing a novel strategy of combining knockdown and overexpression, provide unique molecular insight into the mechanisms of compensation by pro-survival Bcl-2 family proteins. Copyright © 2014 Elsevier Inc. All rights reserved.

12 CFR 263.400 - Scope.

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2010-01-01

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Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263.

PubMed

Guncheva, Maya; Ivanov, Ivaylo; Galunsky, Boris; Stambolieva, Nicolina; Kaneti, Jose

2004-06-01

Kinetic experiments with a substrate series of phenylacetyl-arylamides reveal that at least one polar group in the amine moiety is required for the proper orientation of the substrate in the large nucleophile-binding subsite of penicillin acylase of Escherichia coli. Quantum mechanical molecular modelling of enzyme-substrate interactions in the enzyme active site shows that in the case of substrates lacking local symmetry, the productive binding implies two nonsymmetrical arrangements with respect to the two positively charged guanidinium residues of ArgA145 and ArgB263. This indicates a crucial role of the specified arginine pair in the substrate- and stereoselectivity of penicillin acylase.

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39 CFR 263.5 - Inquiries.

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2014-07-01

... Inquiries. Inquiries regarding records maintenance and disposition should be directed to the Manager, Records Office, United States Postal Service, 475 L'Enfant Plaza, SW., Washington, DC 20260, or, by...

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Code of Federal Regulations, 2011 CFR

2011-07-01

... Inquiries. Inquiries regarding records maintenance and disposition should be directed to the Manager, Records Office, United States Postal Service, 475 L'Enfant Plaza, SW., Washington, DC 20260, or, by...

39 CFR 263.5 - Inquiries.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Inquiries. Inquiries regarding records maintenance and disposition should be directed to the Manager, Records Office, United States Postal Service, 475 L'Enfant Plaza, SW., Washington, DC 20260, or, by...

PMCA2 silencing potentiates MDA-MB-231 breast cancer cell death initiated with the Bcl-2 inhibitor ABT-263.

PubMed

Curry, Merril; Roberts-Thomson, Sarah J; Monteith, Gregory R

2016-09-30

PMCA2 overexpression in some breast cancers suggests that this calcium pump isoform may play a role in breast pathophysiology. To investigate PMCA2 as a potential drug target for breast cancer therapy, we assessed the functional consequence of PMCA2 silencing on cell death pathways and calcium signals in the basal-like MDA-MB-231 breast cancer cell line. Silencing PMCA2 expression alone has no effect on MDA-MB-231 cell viability, however, PMCA2 silencing promotes calcium-induced cell death initiated with the calcium ionophore ionomycin. Assessment of cytoplasmic calcium responses generated with various agents including ionomycin demonstrates that in MDA-MB-231 cells, PMCA2 does not play a major role in shaping global calcium signals. We also examined the ability of PMCA2 silencing to modulate caspase-dependent cell death triggered by a Bcl-2 inhibitor that is in clinical development for the treatment of various cancers, ABT-263 (Navitoclax). Despite the lack of effect on global calcium responses, PMCA2 silencing augmented Bcl-2 inhibitor (ABT-263)-mediated MDA-MB-231 breast cancer cell death. These studies provide evidence that PMCA2 inhibitors could sensitize PMCA2-positive breast cancers to cell death initiators that work through mechanisms involving the Bcl-2 survival pathway. Copyright © 2016 Elsevier Inc. All rights reserved.

[Non iatrogenic primary hypothyrodism in adults at Le Dantec Hospital : clinical features, diagnosis and treatment. Review of 19 cases].

PubMed

Leye, A; Pouye, A; Fall, S; Ndongo, S; Ould Isselmou, El B; Ka, M M; Moreira-Diop, T

2004-01-01

The authors report 19 cases of non iatrogenic primary hypothyroidism in adults at Le Dantec Hospital of Dakar. Those cases had been found during a period of 6 years and half in the internal medicine service. The aim was to study clinical features, diagnosis and outcome of patients after treatment. The mean age of patients was 42.2 years with a sex-ratio of 0.33 M/F. The diagnosis delay was around 6,1 years. All patients presented clinical signs of hypometabolism: physical asthenia (63.15%), frilosity (26.3%), bradycardia (47.3%), constipation (36.8%). The cutaneomucal syndrom was composed by myxoedema (73.6%), macroglossia (26.3%), raucousness of voice (26.3%), alopecia (57.9%). Muscle weakness was found in 2 cases and genital troubles in 3 cases. Five patients presented goiter and 9 others had spontaneous thyroid atrophy. All patients presented a high level of TSH associated with decreased level of T4. Anemia was found in 7 cases and hypercholesterolemia in 13 cases. Treatment was based on substitutive hormonotherapy with L-Thyroxin (75 to 250 microg/day). Evolution was favorable after 10 month mean duration of processing. More alertness is necessary on behalf of the practitioners in front of any sign suggesting hypometabolism to reduce the diagnostic delay and prevent complete form of hypothyroidism that might be complicated, by cardiac involvement in particular.

Assessment of prion reduction filters in decreasing infectivity of ultracentrifuged 263K scrapie-infected brain homogenates in "spiked" human blood and red blood cells.

PubMed

Cardone, Franco; Sowemimo-Coker, Samuel; Abdel-Haq, Hanin; Sbriccoli, Marco; Graziano, Silvia; Valanzano, Angelina; Berardi, Vito Angelo; Galeno, Roberta; Puopolo, Maria; Pocchiari, Maurizio

2014-04-01

The safety of red blood cells (RBCs) is of concern because of the occurrence of four transfusion-transmitted variant Creutzfeldt-Jakob disease (vCJD) cases in the United Kingdom. The absence of validated screening tests requires the use of procedures to remove prions from blood to minimize the risk of transmission. These procedures must be validated using infectious prions in a form that is as close as possible to one in blood. Units of human whole blood (WB) and RBCs were spiked with high-speed supernatants of 263K scrapie-infected hamster brain homogenates. Spiked samples were leukoreduced and then passed through prion-removing filters (Pall Corporation). In another experiment, RBCs from 263K scrapie-infected hamsters were treated as above, and residual infectivity was measured by bioassay. The overall removal of infectivity by the filters from prion-spiked WB and RBCs was approximately two orders of magnitude. No infectivity was detected in filtered hamster RBCs endogenously infected with scrapie. The use of prion-removing filters may help to reduce the risk of transfusion-transmitted vCJD. To avoid overestimation of prion removal efficiency in validation studies, it may be more appropriate to use supernates from ultracentrifugation of scrapie-infected hamster brain homogenate rather than the current standard brain homogenates. © 2013 American Association of Blood Banks.

Proteomic Analysis of Interactions between a Deep-Sea Thermophilic Bacteriophage and Its Host at High Temperature ▿ †

PubMed Central

Wei, Dahai; Zhang, Xiaobo

2010-01-01

The virus-host interaction is essential to understanding the role that viruses play in ecological and geochemical processes in deep-sea vent ecosystems. Virus-induced changes in cellular gene expression and host physiology have been studied extensively. However, the molecular mechanism of interaction between a bacteriophage and its host at high temperature remains poorly understood. In the present study, the virus-induced gene expression profile of Geobacillus sp. E263, a thermophile isolated from a deep-sea hydrothermal ecosystem, was characterized. Based on proteomic analysis and random arbitrarily primed PCR (RAP-PCR) of Geobacillus sp. E263 cultured under non-bacteriophage GVE2 infection and GVE2 infection conditions, there were two types of protein/gene profiles in response to GVE2 infection. Twenty differentially expressed genes and proteins were revealed that could be grouped into 3 different categories based on cellular function, suggesting a coordinated response to infection. These differentially expressed genes and proteins were further confirmed by Northern blot analysis. To characterize the host proteins in response to virus infection, aspartate aminotransferase (AST) was inactivated to construct the AST mutant of Geobacillus sp. E263. The results showed that the AST protein was essential in virus infection. Thus, transcriptional and proteomic analyses and functional analysis revealed previously unknown host responses to deep-sea thermophilic virus infection. PMID:20015994

Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications

PubMed Central

Rémy, Benjamin; Plener, Laure; Poirier, Laetitia; Elias, Mikael; Daudé, David; Chabrière, Eric

2016-01-01

Extremozymes have gained considerable interest as they could meet industrial requirements. Among these, SsoPox is a hyperthermostable enzyme isolated from the archaeon Sulfolobus solfataricus. This enzyme is a lactonase catalyzing the hydrolysis of acyl-homoserine lactones; these molecules are involved in Gram-negative bacterial communication referred to as quorum sensing. SsoPox exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents. Owing to its bi-functional catalytic abilities as well as its intrinsic stability, SsoPox is appealing for many applications, having potential uses in the agriculture, defense, food and health industries. Here we investigate the biotechnological properties of the mutant SsoPox-W263I, a variant with increased lactonase and phosphotriesterase activities. We tested enzyme resistance against diverse process-like and operating conditions such as heat resistance, contact with organic solvents, sterilization, storage and immobilization. Bacterial secreted materials from both Gram-negative and positive bacteria were harmless on SsoPox-W263I activity and could reactivate heat-inactivated enzyme. SsoPox showed resistance to harsh conditions demonstrating that it is an extremely attractive enzyme for many applications. Finally, the potential of SsoPox-W263I to be active at subzero temperature is highlighted and discussed in regards to the common idea that hyperthermophile enzymes are nearly inactive at low temperatures. PMID:27876889

Pacific region influenza surveillance for oseltamivir resistance.

PubMed

Miller, Heather B; Gose, Remedios B; Nagata, Mark T; Sciulli, Rebecca H; Whelen, A Christian

2012-05-01

Hawaii and the United States-affiliated Pacific islands (USAPI) host over 8 million travelers annually, most of whom originate in Asia, Australia, and the Americas where prevalence of oseltamivir resistance in 2009 pandemic influenza A (H1N1) has been reported to be 2.5-3.5%. To survey a collection of samples from Hawaii and the USAPI that had tested positive for the 2009 pandemic influenza A (H1N1) virus by RTI-PCR to assess whether antiviral resistance emerged in these island communities during the 2009 H1N1 pandemic. We examined RNA extracted from Hawaiian and USAPI cases for the neuraminidase H275Y mutation associated with oseltamivir resistance by pyrosequencing. Two hundred and sixty-three (263) 2009 pandemic influenza A (H1N1) positive specimens were tested and 263/263 (100%) were shown to lack the mutation most commonly associated with oseltamivir resistance. There was no evidence of oseltamivir resistant A(H1N1)pdm09 virus during the 2009 pandemic in the Pacific islands despite considerable travel exposure. Geographic isolation, the lack of a "second wave" of pandemic influenza, judicious antiviral use, aggressive vaccination, and below average tourism due to the global economic crisis may have been contributing factors. Continued surveillance and vigilance is necessary to monitor unpredictable influenza activity. Copyright © 2012 Elsevier B.V. All rights reserved.

The Kappa Opioid Agonist U-50, 488H Antagonizes Respiratory Effects of Mu Opioid Receptor Agonists in Conscious Rats

DTIC Science & Technology

1993-01-01

AD 263 043 •1TATION PAGEl ,,, ’, .~- AD- A263 043 §1(d4A 𔃾f *. V A II t~ji lw A I L L A I t C At ( 0 4. ti11 !f AND SUSTITLIf 7 ik’h LiA 6. AUTHOR...and mu antagonistic properties of U-S0,488H were blocked corn- increased arterial PCO2 and blood pressure. Morphine and a low pletely after...I’Nkl IOF AM A (󈧏,00V AND FXP5FHI’-INIAt To IIFHAVFITI)’. Vol , 24, •, *,,iq nr:1ii ,, 11. hv 𔃻’h1. American S .io, fir I’hnrniar ,,.gv and IF rlmn

Patenting and Innovation in China: Incentives, Policy, and Outcomes

DTIC Science & Technology

2015-01-01

database  using a difference‐in‐differences approach with count model specifications. Second, it uses firm‐ level data in a  pretest ‐ posttest  design to... on  the  targeted technology  groups . Firms exhibit increased inventiveness in the year of the award, but  rapid declines in subsequent years, with...Treatment/Control  Groups 263 treatment/263 control    To evaluate the effect  on  innovation of the 2006 announcement of a shift towards an II model—which

75 FR 16204 - Reporting and Recordkeeping Requirements Under OMB Review

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-31

..., Office of Management and Budget, New Executive Office Building, Washington, DC 20503. FOR FURTHER... Burden: 263. Title: Federal Cash Transaction Report, Financial Status Report, Program Income Report...

50 CFR 263.53 - Other funds.

Code of Federal Regulations, 2014 CFR

2014-10-01

... distribute these funds after he or she has made a thorough evaluation of the scientific information submitted... only by existing methods and technology. Any fishery resource used in computing the states' amount...

50 CFR 263.53 - Other funds.

Code of Federal Regulations, 2011 CFR

2011-10-01

... distribute these funds after he or she has made a thorough evaluation of the scientific information submitted... only by existing methods and technology. Any fishery resource used in computing the states' amount...

50 CFR 263.53 - Other funds.

Code of Federal Regulations, 2012 CFR

2012-10-01

... distribute these funds after he or she has made a thorough evaluation of the scientific information submitted... only by existing methods and technology. Any fishery resource used in computing the states' amount...

50 CFR 263.53 - Other funds.

Code of Federal Regulations, 2013 CFR

2013-10-01

... distribute these funds after he or she has made a thorough evaluation of the scientific information submitted... only by existing methods and technology. Any fishery resource used in computing the states' amount...

International Fisheries Stewardship and Enforcement Act

THOMAS, 113th Congress

Sen. Rockefeller, John D., IV [D-WV

2013-02-11

Senate - 12/17/2013 Placed on Senate Legislative Calendar under General Orders. Calendar No. 263. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

26 CFR 1.263A-8 - Requirement to capitalize interest.

Code of Federal Regulations, 2010 CFR

2010-04-01

... must include anticipated expense and time for delay, rework, change orders, and technological, design..., roads, bridges, tunnels, paved parking areas and other pavements, special foundations, wharves and docks...

34 CFR 263.8 - What are the payback requirements?

Code of Federal Regulations, 2010 CFR

2010-07-01

... participant performs. (Approved by the Office of Management and Budget under control number 1810-0580... for which training was actually received under the Professional Development program. (c) The cash...

34 CFR 263.1 - What is the Professional Development program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... become teachers, administrators, teacher aides, social workers, and ancillary educational personnel; and (3) Improve the skills of qualified Indian individuals who serve in the education field. (b) The...

Allelopathy effect of rice straw on the germination and growth of Echinochloa crus-galli (L.) P. Beauv

NASA Astrophysics Data System (ADS)

Anuar, Fitryana Dewi Khairul; Ismail B., S.; Ahmad, Wan Juliana Wan

2015-09-01

A study on the effect of extract and decomposing rice straw of MR220 CL2, MR253 and MR263 on the germination and seedling growth of Echinochloa crus-galli has been conducted in the laboratory and greenhouse of Universiti Kebangsaan Malaysia. Three concentrations of aqueous extract (25, 50 and 100 g L-1) and decomposing rice straw (5, 10 and 15 g 500g-1) were used in the experiment. The experimental design used was the Complete Randomized Design (CRD) to evaluate the allelopathic effect of various concentrations of rice straw on various growth parameters of the test plants. All the experiments were carried out in three replications and conducted twice. Results showed that the rice straw extract of all the varieties showed significant effects on the germination and seedling growth of E. crus-galli. Aqueous extract of MR263 showed the greatest reduction on the germination of E.crus-galli compared to the other varieties at 100 g L-1 concentration (26% as compared to control). As the extract concentration of rice straw increased, the radicle length of E. crus-galli was significantly reduced. The radicle and hypocotyl length of E. crus-galli was significantly inhibited by 82.28% and 41.13% respectively at 100 g L-1 concentration of the aqueous extract of MR263. Decomposing rice straw of all rice varieties inhibited germination and all the growth parameters of the test plants. As the concentration of rice debris increased, the radicle length of the test plant decreased for all treatments. Decomposing rice straw of MR220 CL2 showed the greatest inhibitory effect on the growth of E. crus-galli compared to the other varieties. It inhibited the radicle, hypocotyl, fresh and dry weight of the test plants by 63.29%, 62.61%, 83.68% and 82.49% respectively as compared to the control. Therefore, rice straw of MR220 CL2, MR253 and MR263 showed allelopathic characteristics as they inhibited the germination and various growth parameters of E. crus-galli. However, further studies need to be conducted to determine the mode of action of the allelochemicals involved in rice allelopathy.

49 CFR 234.263 - Relays.

Code of Federal Regulations, 2010 CFR

2010-10-01

... affects the proper functioning of a crossing warning system shall be tested at least once every four years... on four year intervals shall be completed in accordance with the following schedule: (1) Not less...

12 CFR 263.93 - Eligibility to practice.

Code of Federal Regulations, 2010 CFR

2010-01-01

... debarment pursuant to this subpart may practice before the Board. (b) Accountants. Any accountant who is qualified to practice as a certified public accountant or public accountant and is not currently under...

26 CFR 1.263(a)-6 - Election to deduct or capitalize certain expenditures.

Code of Federal Regulations, 2014 CFR

2014-04-01

... expenditures); (2) Section 174 (research and experimental expenditures); (3) Section 175 (soil and water conservation expenditures; endangered species recovery expenditures); (4) Section 179 (election to expense...

33 CFR 263.18 - Program completion-time objectives.

Code of Federal Regulations, 2010 CFR

2010-07-01

... submission of funding request or negative report to OCE 2 1 2 (b) Completion of feasibility study by... these objectives is encouraged for specific studies and projects when appropriate. However, high...

33 CFR 263.18 - Program completion-time objectives.

Code of Federal Regulations, 2011 CFR

2011-07-01

... submission of funding request or negative report to OCE 2 1 2 (b) Completion of feasibility study by... these objectives is encouraged for specific studies and projects when appropriate. However, high...

26 CFR 1.263A-8 - Requirement to capitalize interest.

Code of Federal Regulations, 2011 CFR

2011-04-01

... orders, and technological, design or other problems. To the extent that several distinct activities... time, such as swimming pools, roads, bridges, tunnels, paved parking areas and other pavements, special...

26 CFR 1.263A-8 - Requirement to capitalize interest.

Code of Federal Regulations, 2014 CFR

2014-04-01

... orders, and technological, design or other problems. To the extent that several distinct activities... time, such as swimming pools, roads, bridges, tunnels, paved parking areas and other pavements, special...

26 CFR 1.263A-8 - Requirement to capitalize interest.

Code of Federal Regulations, 2013 CFR

2013-04-01

... orders, and technological, design or other problems. To the extent that several distinct activities... time, such as swimming pools, roads, bridges, tunnels, paved parking areas and other pavements, special...

26 CFR 1.263A-8 - Requirement to capitalize interest.

Code of Federal Regulations, 2012 CFR

2012-04-01

... orders, and technological, design or other problems. To the extent that several distinct activities... time, such as swimming pools, roads, bridges, tunnels, paved parking areas and other pavements, special...

12 CFR 263.93 - Eligibility to practice.

Code of Federal Regulations, 2011 CFR

2011-01-01

... debarment pursuant to this subpart may practice before the Board. (b) Accountants. Any accountant who is qualified to practice as a certified public accountant or public accountant and is not currently under...

12 CFR 263.93 - Eligibility to practice.

Code of Federal Regulations, 2013 CFR

2013-01-01

... debarment pursuant to this subpart may practice before the Board. (b) Accountants. Any accountant who is qualified to practice as a certified public accountant or public accountant and is not currently under...

12 CFR 263.93 - Eligibility to practice.

Code of Federal Regulations, 2012 CFR

2012-01-01

... debarment pursuant to this subpart may practice before the Board. (b) Accountants. Any accountant who is qualified to practice as a certified public accountant or public accountant and is not currently under...

12 CFR 263.93 - Eligibility to practice.

Code of Federal Regulations, 2014 CFR

2014-01-01

... debarment pursuant to this subpart may practice before the Board. (b) Accountants. Any accountant who is qualified to practice as a certified public accountant or public accountant and is not currently under...

12 CFR 263.39 - Exceptions to recommended decision.

Code of Federal Regulations, 2014 CFR

2014-01-01

... exceptions and briefs in support of exceptions must set forth page or paragraph references to the specific parts of the administrative law judge's recommendations to which exception is taken, the page or...

12 CFR 263.39 - Exceptions to recommended decision.

Code of Federal Regulations, 2010 CFR

2010-01-01

... exceptions and briefs in support of exceptions must set forth page or paragraph references to the specific parts of the administrative law judge's recommendations to which exception is taken, the page or...

25 CFR 141.1 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-04-01

... part is to prescribe rules for the regulation of reservation businesses for the protection of Indian consumers on the Navajo, Hopi and Zuni Reservations as required by 25 U.S.C. 261, 262, 263, and 264. ...

American Medical Isotopes Production Act of 2010

THOMAS, 111th Congress

Rep. Markey, Edward J. [D-MA-7

2009-07-21

Senate - 01/28/2010 Placed on Senate Legislative Calendar under General Orders. Calendar No. 263. (All Actions) Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

34 CFR 263.3 - What definitions apply to the Professional Development program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... improve performance, (2) Access to research materials and information on teaching and learning, (3... collaboration, feedback, and peer networking and support. In-service training means professional activities and...

10 CFR 431.263 - Materials incorporated by reference.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Society for Testing and Materials (ASTM) Standard F2324-03, “Standard Test Method for Prerinse Spray..., Forrestal Building, Room 1J-018 (Resource Room of the Building Technologies Program), 1000 Independence...

10 CFR 431.263 - Materials incorporated by reference.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Society for Testing and Materials (ASTM) Standard F2324-03, “Standard Test Method for Prerinse Spray..., Forrestal Building, Room 1J-018 (Resource Room of the Building Technologies Program), 1000 Independence...

12 CFR 263.24 - Scope of document discovery.

Code of Federal Regulations, 2010 CFR

2010-01-01

... the principles of common law provide. (d) Time limits. All discovery, including all responses to... finds on the record that good cause exists for waiving the requirements of this paragraph. [56 FR 38052...

26 CFR 1.162-1 - Business expenses.

Code of Federal Regulations, 2013 CFR

2013-04-01

... included in business expenses are management expenses, commissions (but see section 263 and the regulations... from such business. In the case of any sports program to which section 114 (relating to sports programs...

26 CFR 1.162-1 - Business expenses.

Code of Federal Regulations, 2012 CFR

2012-04-01

... included in business expenses are management expenses, commissions (but see section 263 and the regulations... from such business. In the case of any sports program to which section 114 (relating to sports programs...

26 CFR 1.162-1 - Business expenses.

Code of Federal Regulations, 2011 CFR

2011-04-01

... included in business expenses are management expenses, commissions (but see section 263 and the regulations... from such business. In the case of any sports program to which section 114 (relating to sports programs...

26 CFR 1.162-1 - Business expenses.

Code of Federal Regulations, 2014 CFR

2014-04-01

... included in business expenses are management expenses, commissions (but see section 263 and the regulations... from such business. In the case of any sports program to which section 114 (relating to sports programs...

Bcl-XL represents a druggable molecular vulnerability during aurora B inhibitor-mediated polyploidization.

PubMed

Shah, O Jameel; Lin, Xiaoyu; Li, Leiming; Huang, Xiaoli; Li, Junling; Anderson, Mark G; Tang, Hua; Rodriguez, Luis E; Warder, Scott E; McLoughlin, Shaun; Chen, Jun; Palma, Joann; Glaser, Keith B; Donawho, Cherrie K; Fesik, Stephen W; Shen, Yu

2010-07-13

Aurora kinase B inhibitors induce apoptosis secondary to polyploidization and have entered clinical trials as an emerging class of neocytotoxic chemotherapeutics. We demonstrate here that polyploidization neutralizes Mcl-1 function, rendering cancer cells exquisitely dependent on Bcl-XL/-2. This "addiction" can be exploited therapeutically by combining aurora kinase inhibitors and the orally bioavailable BH3 mimetic, ABT-263, which inhibits Bcl-XL, Bcl-2, and Bcl-w. The combination of ABT-263 with aurora B inhibitors produces a synergistic loss of viability in a range of cell lines of divergent tumor origin and exhibits more sustained tumor growth inhibition in vivo compared with aurora B inhibitor monotherapy. These data demonstrate that Bcl-XL/-2 is necessary to support viability during polyploidization in a variety of tumor models and represents a druggable molecular vulnerability with potential therapeutic utility.

A synthetic peptide targeting the BH4 domain of Bcl-2 induces apoptosis in multiple myeloma and follicular lymphoma cells alone or in combination with agents targeting the BH3-binding pocket of Bcl-2.

PubMed

Lavik, Andrew R; Zhong, Fei; Chang, Ming-Jin; Greenberg, Edward; Choudhary, Yuvraj; Smith, Mitchell R; McColl, Karen S; Pink, John; Reu, Frederic J; Matsuyama, Shigemi; Distelhorst, Clark W

2015-09-29

Bcl-2 inhibits apoptosis by two distinct mechanisms but only one is targeted to treat Bcl-2-positive malignancies. In this mechanism, the BH1-3 domains of Bcl-2 form a hydrophobic pocket, binding and inhibiting pro-apoptotic proteins, including Bim. In the other mechanism, the BH4 domain mediates interaction of Bcl-2 with inositol 1,4, 5-trisphosphate receptors (IP3Rs), inhibiting pro-apoptotic Ca2+ signals. The current anti-Bcl-2 agents, ABT-263 (Navitoclax) and ABT-199 (Venetoclax), induce apoptosis by displacing pro-apoptotic proteins from the hydrophobic pocket, but do not inhibit Bcl-2-IP3R interaction. Therefore, to target this interaction we developed BIRD-2 (Bcl-2 IP3 Receptor Disruptor-2), a decoy peptide that binds to the BH4 domain, blocking Bcl-2-IP3R interaction and thus inducing Ca2+-mediated apoptosis in chronic lymphocytic leukemia, multiple myeloma, and follicular lymphoma cells, including cells resistant to ABT-263, ABT-199, or the Bruton's tyrosine kinase inhibitor Ibrutinib. Moreover, combining BIRD-2 with ABT-263 or ABT-199 enhances apoptosis induction compared to single agent treatment. Overall, these findings provide strong rationale for developing novel therapeutic agents that mimic the action of BIRD-2 in targeting the BH4 domain of Bcl-2 and disrupting Bcl-2-IP3R interaction.

X-linked Acrogigantism (X-LAG) Syndrome: Clinical Profile and Therapeutic Responses

PubMed Central

Beckers, Albert; Lodish, Maya Beth; Trivellin, Giampaolo; Rostomyan, Liliya; Lee, Misu; Faucz, Fabio R; Yuan, Bo; Choong, Catherine S; Caberg, Jean-Hubert; Verrua, Elisa; Naves, Luciana Ansaneli; Cheetham, Tim D; Young, Jacques; Lysy, Philippe A; Petrossians, Patrick; Cotterill, Andrew; Shah, Nalini Samir; Metzger, Daniel; Castermans, Emilie; Ambrosio, Maria Rosaria; Villa, Chiara; Strebkova, Natalia; Mazerkina, Nadia; Gaillard, Stéphan; Barra, Gustavo Barcelos; Casulari, Luis Augusto; Neggers, Sebastian J.; Salvatori, Roberto; Jaffrain-Rea, Marie-Lise; Zacharin, Margaret; Santamaria, Beatriz Lecumberri; Zacharieva, Sabina; Lim, Ee Mun; Mantovani, Giovanna; Zatelli, Maria Chaira; Collins, Michael T; Bonneville, Jean-François; Quezado, Martha; Chittiboina, Prashant; Oldfield, Edward H.; Bours, Vincent; Liu, Pengfei; De Herder, Wouter; Pellegata, Natalia; Lupski, James R.; Daly, Adrian F.; Stratakis, Constantine A.

2015-01-01

X-linked acro-gigantism (X-LAG) is a new syndrome of pituitary gigantism, caused by microduplications on chromosome Xq26.3, encompassing the gene GPR101, which is highly upregulated in pituitary tumors. We conducted this study to explore the clinical, radiological and hormonal phenotype and responses to therapy in patients with X-LAG syndrome. The study included 18 patients (13 sporadic) with X-LAG and a microduplication in chromosome Xq26.3. All sporadic cases had unique duplications and the inheritance pattern in 2 families was dominant with all Xq26.3 duplication carriers being affected. Patients began to grow rapidly as early as 2–3 months of age (median 12 months). At diagnosis (median delay 27 months), patients had a median height and weight SDS score of >+3.9 SDS. Apart from the increased overall body size, the children had acromegalic symptoms including acral enlargement and facial coarsening. More than a third of cases had increased appetite. Patients had marked hypersecretion of GH/IGF-1 and prolactin, usually due to a pituitary macroadenoma or hyperplasia. Primary neurosurgical control was achieved with extensive anterior pituitary resection but postoperative hypopituitarism was frequent. Control with somatostatin analogs was not readily achieved despite moderate to high somatostatin receptor subtype-2 expression in tumor tissue. Postoperative adjuvant pegvisomant achieved control of IGF-1 all 5 cases in which it was employed. X-LAG is a new infant-onset gigantism syndrome that has a severe clinical phenotype leading to challenging disease management. PMID:25712922

Mutational scanning of the human serotonin transporter reveals fast translocating serotonin transporter mutants.

PubMed

Kristensen, Anders S; Larsen, Mads B; Johnsen, Laust B; Wiborg, Ove

2004-03-01

The serotonin transporter (SERT) belongs to a family of sodium-chloride-dependent transporters responsible for uptake of amino acids and biogenic amines from the extracellular space. SERT represents a major pharmacological target in the treatment of several clinical conditions, including depression and anxiety. In the present study we have undertaken a mutational scanning of human SERT in order to identify residues that are responsible for individual differences among related monoamine transporters. One mutant, G100A, was inactive in transport. However, ligand binding affinity was similar to wild-type, suggesting that G100A amongst different possible SERT conformations is restrained to a binding conformation. We suggest that the main role of glycine-100 is to confer structural flexibility during substrate translocation. For the two single mutants, T178A and F263C, uptake rates and K(m) values were both several-fold higher than wild-type while binding affinities and inhibitory potencies decreased considerably for several drugs. Ion dependency increased and only at hyperosmotic concentrations were K(m) values partly restored. For the double mutant, T178A/F263C, shifts in uptake kinetics and ligand affinities, as well as ion dependencies, were drastic. Effects were synergistic compared to the corresponding single mutants. In conclusion, we suggest that mutating threonine-178 to an alanine and phenylalanine-263 to a cysteine mainly alter the overall uptake kinetics of SERT by affecting the conformational equilibrium of different transporter conformations.

34 CFR 263.21 - What priority is given to certain projects and applicants?

Code of Federal Regulations, 2010 CFR

2010-07-01

... successful entry into school at the kindergarten school level. (2) Early childhood and kindergarten programs... subject matters, including math and science, to enable Indian students to successfully transition to...

HOMER® Energy Modeling Software V2.63

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lambert, Tom

2003-12-31

The HOMER® energy modeling software is a tool for designing and analyzing hybrid power systems, which contain a mix of conventional generators, cogeneration, wind turbines, solar photovoltaic, hydropower, batteries, fuel cells, hydropower, biomass and other inputs.

45 CFR 263.20 - What definitions apply to Individual Development Accounts (IDAs)?

Code of Federal Regulations, 2013 CFR

2013-10-01

... FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH... Carl D. Perkins Vocational and Applied Technology Education Act (20 U.S.C. 2471(4)) that is in any...

45 CFR 263.20 - What definitions apply to Individual Development Accounts (IDAs)?

Code of Federal Regulations, 2010 CFR

2010-10-01

... FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH... Carl D. Perkins Vocational and Applied Technology Education Act (20 U.S.C. 2471(4)) that is in any...

45 CFR 263.20 - What definitions apply to Individual Development Accounts (IDAs)?

Code of Federal Regulations, 2014 CFR

2014-10-01

... FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH... Carl D. Perkins Vocational and Applied Technology Education Act (20 U.S.C. 2471(4)) that is in any...

45 CFR 263.20 - What definitions apply to Individual Development Accounts (IDAs)?

Code of Federal Regulations, 2011 CFR

2011-10-01

... FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH... Carl D. Perkins Vocational and Applied Technology Education Act (20 U.S.C. 2471(4)) that is in any...

45 CFR 263.20 - What definitions apply to Individual Development Accounts (IDAs)?

Code of Federal Regulations, 2012 CFR

2012-10-01

... FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH... Carl D. Perkins Vocational and Applied Technology Education Act (20 U.S.C. 2471(4)) that is in any...

27 CFR 46.263 - Conditions for approval.

Code of Federal Regulations, 2010 CFR

2010-04-01

... CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette... any provision of law; (e) It will not result in an increase in cost to the Government; (f) It will not...

26 CFR 1.263(a)-6T - Election to deduct or capitalize certain expenditures (temporary).

Code of Federal Regulations, 2012 CFR

2012-04-01

... 195 (start-up expenditures); (16) Section 198 (expensing of environmental remediation costs); (17... environmental protection agency sulfur regulations); (7) Section 179C (election to expense certain refineries...

26 CFR 1.263(a)-6T - Election to deduct or capitalize certain expenditures (temporary).

Code of Federal Regulations, 2013 CFR

2013-04-01

... 195 (start-up expenditures); (16) Section 198 (expensing of environmental remediation costs); (17... environmental protection agency sulfur regulations); (7) Section 179C (election to expense certain refineries...

27 CFR 46.263 - Conditions for approval.

Code of Federal Regulations, 2013 CFR

2013-04-01

... CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette... any provision of law; (e) It will not result in an increase in cost to the Government; (f) It will not...

27 CFR 46.263 - Conditions for approval.

Code of Federal Regulations, 2011 CFR

2011-04-01

... CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette... any provision of law; (e) It will not result in an increase in cost to the Government; (f) It will not...

27 CFR 46.263 - Conditions for approval.

Code of Federal Regulations, 2012 CFR

2012-04-01

... CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette... any provision of law; (e) It will not result in an increase in cost to the Government; (f) It will not...

27 CFR 46.263 - Conditions for approval.

Code of Federal Regulations, 2014 CFR

2014-04-01

... CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette... any provision of law; (e) It will not result in an increase in cost to the Government; (f) It will not...

Work-Centered Technology Development (WTD)

DTIC Science & Technology

2005-03-01

theoretical, testable, inductive, and repeatable foundations of science. o Theoretical foundations include notions such as statistical versus analytical...Human Factors and Ergonomics Society, 263-267. 179 Eggleston, R. G. (2005). Coursebook : Work-Centered Design (WCD). AFRL/HECS WCD course training

29 CFR 780.1016 - Use of evergreens and forest products.

Code of Federal Regulations, 2010 CFR

2010-07-01

... the purpose for which evergreens may be harvested under section 13(d). Harvesting of these materials... evergreens are used for wreathmaking by persons other than the homeworkers (see Mitchell v. Hunt, 263 F. 2d...

29 CFR 780.1016 - Use of evergreens and forest products.

Code of Federal Regulations, 2014 CFR

2014-07-01

... the purpose for which evergreens may be harvested under section 13(d). Harvesting of these materials... evergreens are used for wreathmaking by persons other than the homeworkers (see Mitchell v. Hunt, 263 F. 2d...

29 CFR 780.1016 - Use of evergreens and forest products.

Code of Federal Regulations, 2012 CFR

2012-07-01

... the purpose for which evergreens may be harvested under section 13(d). Harvesting of these materials... evergreens are used for wreathmaking by persons other than the homeworkers (see Mitchell v. Hunt, 263 F. 2d...

29 CFR 780.1016 - Use of evergreens and forest products.

Code of Federal Regulations, 2013 CFR

2013-07-01

... the purpose for which evergreens may be harvested under section 13(d). Harvesting of these materials... evergreens are used for wreathmaking by persons other than the homeworkers (see Mitchell v. Hunt, 263 F. 2d...

29 CFR 780.1016 - Use of evergreens and forest products.

Code of Federal Regulations, 2011 CFR

2011-07-01

... the purpose for which evergreens may be harvested under section 13(d). Harvesting of these materials... evergreens are used for wreathmaking by persons other than the homeworkers (see Mitchell v. Hunt, 263 F. 2d...

Alternative strategy for converting an inverting glycoside hydrolase into a glycosynthase.

PubMed

Honda, Yuji; Fushinobu, Shinya; Hidaka, Masafumi; Wakagi, Takayoshi; Shoun, Hirofumi; Taniguchi, Hajime; Kitaoka, Motomitsu

2008-04-01

The tyrosine residue Y198 is known to support a nucleophilic water molecule with the general base residue, D263, in the reducing-end xylose-releasing exo-oligoxylanase (Rex). A mutation in the tyrosine residue changing it into phenylalanine caused a drastic decrease in the hydrolytic activity and a small increase in the F(-) releasing activity from alpha-xylobiosyl fluoride in the presence of xylose. In contrast, mutations at D263 resulted in the decreased F(-) releasing activity. As a result of the high F(-) releasing activity and low hydrolytic activity, Y198F of Rex accumulates a large amount of product during the glycosynthase reaction. We propose a novel method for producing a glycosynthase from an inverting glycoside hydrolase by mutating a residue that holds the nucleophilic water molecule with the general base residue while keeping the general base residue intact.

Clinical Characterization of Canine Acanthomatous Ameloblastoma (CAA) in 263 dogs and the Influence of Postsurgical Histopathological Margin on Local Recurrence.

PubMed

Goldschmidt, Stephanie L; Bell, Cindy M; Hetzel, Scott; Soukup, Jason

2017-12-01

Canine acanthomatous ameloblastoma (CAA) has been reported to be the most common odontogenic tumor in dogs. This retrospective study evaluated 263 dogs with histopathologically confirmed CAA. Within this data set, CAA presents most commonly in the rostral mandible in adult large breed dogs, with golden retriever dogs being overrepresented. Patients with appropriate follow-up after curative intent surgery were evaluated to assess the effect of histopathological margin on local tumor recurrence. No local recurrence was noted in any patient. This study raises questions about what the recommended surgical margin should be for treatment of CAA. It also serves as a stimulus for discussion as to whether further treatment for CAA is required when inadequate surgical margins are obtained, or if medical surveillance would be an appropriate management recommendation. Prospective studies are necessary to answer these questions.

Evaluating the effectiveness of SW-only video coding for real-time video transmission over low-rate wireless networks

NASA Astrophysics Data System (ADS)

Bartolini, Franco; Pasquini, Cristina; Piva, Alessandro

2001-04-01

The recent development of video compression algorithms allowed the diffusion of systems for the transmission of video sequences over data networks. However, the transmission over error prone mobile communication channels is yet an open issue. In this paper, a system developed for the real time transmission of H263 video coded sequences over TETRA mobile networks is presented. TETRA is an open digital trunked radio standard defined by the European Telecommunications Standardization Institute developed for professional mobile radio users, providing full integration of voice and data services. Experimental tests demonstrate that, in spite of the low frame rate allowed by the SW only implementation of the decoder and by the low channel rate a video compression technique such as that complying with the H263 standard, is still preferable to a simpler but less effective frame based compression system.

Bcl-XL represents a druggable molecular vulnerability during aurora B inhibitor-mediated polyploidization

PubMed Central

Shah, O. Jameel; Lin, Xiaoyu; Li, Leiming; Huang, Xiaoli; Li, Junling; Anderson, Mark G.; Tang, Hua; Rodriguez, Luis E.; Warder, Scott E.; McLoughlin, Shaun; Chen, Jun; Palma, Joann; Glaser, Keith B.; Donawho, Cherrie K.; Fesik, Stephen W.; Shen, Yu

2010-01-01

Aurora kinase B inhibitors induce apoptosis secondary to polyploidization and have entered clinical trials as an emerging class of neocytotoxic chemotherapeutics. We demonstrate here that polyploidization neutralizes Mcl-1 function, rendering cancer cells exquisitely dependent on Bcl-XL/-2. This “addiction” can be exploited therapeutically by combining aurora kinase inhibitors and the orally bioavailable BH3 mimetic, ABT-263, which inhibits Bcl-XL, Bcl-2, and Bcl-w. The combination of ABT-263 with aurora B inhibitors produces a synergistic loss of viability in a range of cell lines of divergent tumor origin and exhibits more sustained tumor growth inhibition in vivo compared with aurora B inhibitor monotherapy. These data demonstrate that Bcl-XL/-2 is necessary to support viability during polyploidization in a variety of tumor models and represents a druggable molecular vulnerability with potential therapeutic utility. PMID:20616035

Synthesis, structure and electrical properties of Cu 3.21Ti 1.16Nb 2.63O 12 and the CuO x-TiO 2-Nb 2O 5 pseudoternary phase diagram

NASA Astrophysics Data System (ADS)

Reeves-McLaren, Nik; Ferrarelli, Matthew C.; Tung, Yuan-Wei; Sinclair, Derek C.; West, Anthony R.

2011-07-01

Subsolidus phase relations in the CuO x-TiO 2-Nb 2O 5 system were determined at 935 °C. The phase diagram contains one new phase, Cu 3.21Ti 1.16Nb 2.63O 12 (CTNO) and one rutile-structured solid solution series, Ti 1-3 xCu xNb 2 xO 2: 0< x<0.2335 (35). The crystal structure of CTNO is similar to that of CaCu 3Ti 4O 12 (CCTO) with square planar Cu 2+ but with A site vacancies and a disordered mixture of Cu +, Ti 4+ and Nb 5+ on the octahedral sites. It is a modest semiconductor with relative permittivity ˜63 and displays non-Arrhenius conductivity behavior that is essentially temperature-independent at the lowest temperatures.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Froula, D. H.; Boni, R.; Bedzyk, M.

A 10-ps, 263-nm (4{omega}) laser is being built to probe plasmas produced on the OMEGA EP [J. H. Kelly, L. J. Waxer, V. Bagnoud, I. A. Begishev, J. Bromage, B. E. Kruschwitz, T. E. Kessler, S. J. Loucks, D. N. Maywar, R. L. McCrory et al., J. Phys. IV France 133, 75-80 (2006)]. A suite of optical diagnostics (schlieren, interferometry, and grid image refractometry) has been designed to diagnose and characterize a wide variety of plasmas. Light scattered by the probe beam is collected by an f/4 catadioptric telescope and a transport system is designed to image with a near-diffraction-limitedmore » resolution ({approx}1 -{mu}m full width at half maximum) over a 5-mm field of view to a diagnostic table. The transport system provides a contrast greater than 1 : 10{sup 4} with respect to all wavelengths outside of the 263 {+-} 2 nm measurement range.« less

76 FR 81059 - Guidance Regarding Deduction and Capitalization of Expenditures Related to Tangible Property

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-27

...This document contains temporary regulations that provide guidance on the application of sections 162(a) and 263(a) of the Internal Revenue Code to amounts paid to acquire, produce, or improve tangible property. The temporary regulations clarify and expand the standards in the current regulations under sections 162(a) and 263(a) and provide certain bright-line tests (for example, a de minimis rule for certain acquisitions) for applying these standards. The temporary regulations also provide guidance under section 168 regarding the accounting for, and dispositions of, property subject to section 168. The temporary regulations also amend the general asset account regulations. The temporary regulations will affect all taxpayers that acquire, produce, or improve tangible property. The text of the temporary regulations also serves as the text of proposed regulations set forth in the notice of proposed rulemaking on this subject appearing elsewhere in this issue of the Federal Register.

Sodium taurocholate cotransporting polypeptide (NTCP) deficiency: Identification of a novel SLC10A1 mutation in two unrelated infants presenting with neonatal indirect hyperbilirubinemia and remarkable hypercholanemia

PubMed Central

Qiu, Jian-Wu; Deng, Mei; Cheng, Ying; Atif, Raza-Muhammad; Lin, Wei-Xia; Guo, Li; Li, Hua; Song, Yuan-Zong

2017-01-01

Sodium taurocholate cotransporting polypeptide (NTCP) is encoded by the gene SLC10A1 and expressed in the basolateral membrane of the hepatocyte, functioning to uptake bile acids from plasma. Although SLC10A1 has been cloned and NTCP function studied intensively for years, clinical description of NTCP deficiency remains rather limited. This study reported the genotypic and phenotypic features of two neonatal patients with NTCP deficiency. They both presented with neonatal indirect hyperbilirubinemia and remarkable hypercholanemia, and harbored the SLC10A1 variants c.800C>T (p.S267F) and c.263T>C (p.I88T). On genetic analysis of the two family trios, the latter missense variant was detected in trans with the former, a reported loss-of-function variant. Having not been reported in any databases, the c.263T>C (p.I88T) variant demonstrated an allele frequency of 0.67% (1/150) in healthy controls. Moreover, this variant involved a relatively conservative amino acid, and was predicted to be pathogenic or deleterious by changing the conformation of the NTCP molecule. In conclusion, the novel variant c.263T>C (p.I88T) in this study enriched the SLC10A1 mutation spectrum; the clinical findings lent support to the primary role of NTCP in hepatic bile acid clearance, and suggested that NTCP deficiency might be a contributing factor for the development of neonatal indirect hyperbilirubinemia. PMID:29290974

Sodium taurocholate cotransporting polypeptide (NTCP) deficiency: Identification of a novel SLC10A1 mutation in two unrelated infants presenting with neonatal indirect hyperbilirubinemia and remarkable hypercholanemia.

PubMed

Qiu, Jian-Wu; Deng, Mei; Cheng, Ying; Atif, Raza-Muhammad; Lin, Wei-Xia; Guo, Li; Li, Hua; Song, Yuan-Zong

2017-12-05

Sodium taurocholate cotransporting polypeptide (NTCP) is encoded by the gene SLC10A1 and expressed in the basolateral membrane of the hepatocyte, functioning to uptake bile acids from plasma. Although SLC10A1 has been cloned and NTCP function studied intensively for years, clinical description of NTCP deficiency remains rather limited. This study reported the genotypic and phenotypic features of two neonatal patients with NTCP deficiency. They both presented with neonatal indirect hyperbilirubinemia and remarkable hypercholanemia, and harbored the SLC10A1 variants c.800C>T (p.S267F) and c.263T>C (p.I88T). On genetic analysis of the two family trios, the latter missense variant was detected in trans with the former, a reported loss-of-function variant. Having not been reported in any databases, the c.263T>C (p.I88T) variant demonstrated an allele frequency of 0.67% (1/150) in healthy controls. Moreover, this variant involved a relatively conservative amino acid, and was predicted to be pathogenic or deleterious by changing the conformation of the NTCP molecule. In conclusion, the novel variant c.263T>C (p.I88T) in this study enriched the SLC10A1 mutation spectrum; the clinical findings lent support to the primary role of NTCP in hepatic bile acid clearance, and suggested that NTCP deficiency might be a contributing factor for the development of neonatal indirect hyperbilirubinemia.

Amor: Investigating The Triple Asteroid System 2001 SN263

NASA Astrophysics Data System (ADS)

Jones, T.; Bellerose, Julie; Lee, P.; Prettyman, T.; Lawrence, D.; Smith, P.; Gaffey, M.; Nolan, M.; Goldsten, J.; Thomas, P.; Veverka, J.; Farquhar, R.; Heldmann, J.; Reddy, V.; Williams, B.; Chartres, J.; DeRosee, R.; Dunham, D.

2010-10-01

The Amor mission will rendezvous and land at the triple Near-Earth Asteroid system (153591) 2001 SN263 and execute detailed, in-situ science investigations. The spacecraft reaches 2001 SN263 by using a two-year ΔVEGA (ΔV-Earth Gravity Assist) trajectory with a relatively low launch C3 of 33.5 km2/s2. Rendezvous will enable reconnaissance activities including global and regional imaging, shape modeling, system dynamics, and compositional mapping. After landing, Amor will conduct in-situ imaging (panoramic to microscopic scale) and compositional measurements to include elemental abundance. The main objectives are to 1) establish in-situ the long-hypothesized link between C-type asteroids and the primitive carbonaceous chondrite (CC) meteorites, 2) investigate the nature, origin and evolution of C-type asteroids, and 3) investigate the origin and evolution of a multiple asteroid system. The mission also addresses the distribution of volatiles and organic materials, impact hazards, and resources for future exploration. Amor is managed by NASA Ames Research Center in partnership with Orbital Sciences, KinetX, MDA, and Draper with heritage instruments provided by Ball Aerospace, JHU/APL, and Firestar Engineering. The science team brings experience from NEAR, Hayabusa, Deep Impact, Dawn, LCROSS, Kepler, and Mars missions. In this paper, we describe the science, mission design, and main operational challenges of performing in-situ science at this triple asteroid system. Challenges include landing on the asteroid components, thermal environment, short day-night cycles, and the operation of deployed instruments in a low gravity (10^-5 g) environment.

Run 263 with Black Thunder Mine subbituminous coal and dispersed molybdenum catalysts. Technical progress report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This report presents the results of Run 263 performed at the Advanced Coal Liquefaction R&D Facility in Wilsonville, Alabama. The run started on October 31, 1991 and continued until February 23, 1992. Tests were conducted by operating the reactors in the Close-Coupled Integrated Two-Stage Liquefaction mode and by processing Black Thunder Mine subbituminous coal from Wyodak-Anderson seam in Wyoming Powder River Basin. Half volume reactors were used for the entire run. In the first part of Run 263, a dispersed molybdenum catalyst was evaluated for its performance without a supported catalyst in the second stage. Molyvan L and Molyvan 822more » (commercially available as friction reducing lubricants) were used as precursors for the dispersed molybdenum catalyst. The effect of the dispersed catalyst on eliminating the solids buildup was also evaluated. For the second part of the run, the hybrid catalyst system was tested with supported Criterion 324 1/1611 catalyst in the second stage at catalyst replacement rates of 2 and 3 lb/ton of MF coal. The molybdenum concentration was 100--200 ppm based on MF coal. Iron oxide was used as a slurry catalyst precursor at a rate of 1--2 wt % MF coal throughout the run with dimethyl disulfide (DMDS) as the sulfiding agent. The close-coupled reactor unit was on-stream for 2482 hours for an on-stream factor of 91.2% and the ROSE-SR{sup sm} unit was on-feed for 2126 hours for an on-stream factor of 96.4% for the entire run.« less

Run 263 with Black Thunder Mine subbituminous coal and dispersed molybdenum catalysts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This report presents the results of Run 263 performed at the Advanced Coal Liquefaction R D Facility in Wilsonville, Alabama. The run started on October 31, 1991 and continued until February 23, 1992. Tests were conducted by operating the reactors in the Close-Coupled Integrated Two-Stage Liquefaction mode and by processing Black Thunder Mine subbituminous coal from Wyodak-Anderson seam in Wyoming Powder River Basin. Half volume reactors were used for the entire run. In the first part of Run 263, a dispersed molybdenum catalyst was evaluated for its performance without a supported catalyst in the second stage. Molyvan L and Molyvanmore » 822 (commercially available as friction reducing lubricants) were used as precursors for the dispersed molybdenum catalyst. The effect of the dispersed catalyst on eliminating the solids buildup was also evaluated. For the second part of the run, the hybrid catalyst system was tested with supported Criterion 324 1/1611 catalyst in the second stage at catalyst replacement rates of 2 and 3 lb/ton of MF coal. The molybdenum concentration was 100--200 ppm based on MF coal. Iron oxide was used as a slurry catalyst precursor at a rate of 1--2 wt % MF coal throughout the run with dimethyl disulfide (DMDS) as the sulfiding agent. The close-coupled reactor unit was on-stream for 2482 hours for an on-stream factor of 91.2% and the ROSE-SR[sup sm] unit was on-feed for 2126 hours for an on-stream factor of 96.4% for the entire run.« less

X-linked acrogigantism syndrome: clinical profile and therapeutic responses.

PubMed

Beckers, Albert; Lodish, Maya Beth; Trivellin, Giampaolo; Rostomyan, Liliya; Lee, Misu; Faucz, Fabio R; Yuan, Bo; Choong, Catherine S; Caberg, Jean-Hubert; Verrua, Elisa; Naves, Luciana Ansaneli; Cheetham, Tim D; Young, Jacques; Lysy, Philippe A; Petrossians, Patrick; Cotterill, Andrew; Shah, Nalini Samir; Metzger, Daniel; Castermans, Emilie; Ambrosio, Maria Rosaria; Villa, Chiara; Strebkova, Natalia; Mazerkina, Nadia; Gaillard, Stéphan; Barra, Gustavo Barcelos; Casulari, Luis Augusto; Neggers, Sebastian J; Salvatori, Roberto; Jaffrain-Rea, Marie-Lise; Zacharin, Margaret; Santamaria, Beatriz Lecumberri; Zacharieva, Sabina; Lim, Ee Mun; Mantovani, Giovanna; Zatelli, Maria Chaira; Collins, Michael T; Bonneville, Jean-François; Quezado, Martha; Chittiboina, Prashant; Oldfield, Edward H; Bours, Vincent; Liu, Pengfei; W de Herder, Wouter; Pellegata, Natalia; Lupski, James R; Daly, Adrian F; Stratakis, Constantine A

2015-06-01

X-linked acrogigantism (X-LAG) is a new syndrome of pituitary gigantism, caused by microduplications on chromosome Xq26.3, encompassing the gene GPR101, which is highly upregulated in pituitary tumors. We conducted this study to explore the clinical, radiological, and hormonal phenotype and responses to therapy in patients with X-LAG syndrome. The study included 18 patients (13 sporadic) with X-LAG and microduplication of chromosome Xq26.3. All sporadic cases had unique duplications and the inheritance pattern in two families was dominant, with all Xq26.3 duplication carriers being affected. Patients began to grow rapidly as early as 2-3 months of age (median 12 months). At diagnosis (median delay 27 months), patients had a median height and weight standard deviation scores (SDS) of >+3.9 SDS. Apart from the increased overall body size, the children had acromegalic symptoms including acral enlargement and facial coarsening. More than a third of cases had increased appetite. Patients had marked hypersecretion of GH/IGF1 and usually prolactin, due to a pituitary macroadenoma or hyperplasia. Primary neurosurgical control was achieved with extensive anterior pituitary resection, but postoperative hypopituitarism was frequent. Control with somatostatin analogs was not readily achieved despite moderate to high levels of expression of somatostatin receptor subtype-2 in tumor tissue. Postoperative use of adjuvant pegvisomant resulted in control of IGF1 in all five cases where it was employed. X-LAG is a new infant-onset gigantism syndrome that has a severe clinical phenotype leading to challenging disease management. © 2015 Society for Endocrinology.

Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinical Aerobic Gram-Negative Bacterial Isolates

PubMed Central

Faron, Matthew L.; Buchan, Blake W.; Hyke, Josh; Madisen, Neil; Lillie, Jennifer L.; Granato, Paul A.; Wilson, Deborah A.; Procop, Gary W.; Novak-Weekley, Susan; Marlowe, Elizabeth; Cumpio, Joven; Griego-Fullbright, Christen; Kindig, Sandra; Timm, Karen; Young, Stephen; Ledeboer, Nathan A.

2015-01-01

The prompt and accurate identification of bacterial pathogens is fundamental to patient health and outcome. Recent advances in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have revolutionized bacterial identification in the clinical laboratory, but uniform incorporation of this technology in the U.S. market has been delayed by a lack of FDA-cleared systems. In this study, we conducted a multicenter evaluation of the MALDI Biotyper CA (MBT-CA) System (Bruker Daltonics Inc, Billerica, MA) for the identification of aerobic gram-negative bacteria as part of a 510(k) submission to the FDA. A total of 2,263 aerobic gram negative bacterial isolates were tested representing 23 genera and 61 species. Isolates were collected from various clinical sources and results obtained from the MBT-CA System were compared to DNA sequencing and/or biochemical testing. Isolates that failed to report as a "high confidence species ID" [log(score) ≥2.00] were re-tested using an extraction method. The MBT-CA System identified 96.8% and 3.1% of isolates with either a "high confidence" or a "low confidence" [log(score) value between 1.70 and <2.00] species ID, respectively. Two isolates did not produce acceptable confidence scores after extraction. The MBT-CA System correctly identified 99.8% (2,258/2,263) to genus and 98.2% (2,222/2,263) to species level. These data demonstrate that the MBT-CA System provides accurate results for the identification of aerobic gram-negative bacteria. PMID:26529504

Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinical Aerobic Gram-Negative Bacterial Isolates.

PubMed

Faron, Matthew L; Buchan, Blake W; Hyke, Josh; Madisen, Neil; Lillie, Jennifer L; Granato, Paul A; Wilson, Deborah A; Procop, Gary W; Novak-Weekley, Susan; Marlowe, Elizabeth; Cumpio, Joven; Griego-Fullbright, Christen; Kindig, Sandra; Timm, Karen; Young, Stephen; Ledeboer, Nathan A

2015-01-01

The prompt and accurate identification of bacterial pathogens is fundamental to patient health and outcome. Recent advances in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have revolutionized bacterial identification in the clinical laboratory, but uniform incorporation of this technology in the U.S. market has been delayed by a lack of FDA-cleared systems. In this study, we conducted a multicenter evaluation of the MALDI Biotyper CA (MBT-CA) System (Bruker Daltonics Inc, Billerica, MA) for the identification of aerobic gram-negative bacteria as part of a 510(k) submission to the FDA. A total of 2,263 aerobic gram negative bacterial isolates were tested representing 23 genera and 61 species. Isolates were collected from various clinical sources and results obtained from the MBT-CA System were compared to DNA sequencing and/or biochemical testing. Isolates that failed to report as a "high confidence species ID" [log(score) ≥2.00] were re-tested using an extraction method. The MBT-CA System identified 96.8% and 3.1% of isolates with either a "high confidence" or a "low confidence" [log(score) value between 1.70 and <2.00] species ID, respectively. Two isolates did not produce acceptable confidence scores after extraction. The MBT-CA System correctly identified 99.8% (2,258/2,263) to genus and 98.2% (2,222/2,263) to species level. These data demonstrate that the MBT-CA System provides accurate results for the identification of aerobic gram-negative bacteria.

43 CFR 2.63 - Requests for access to records: Submission.

Code of Federal Regulations, 2010 CFR

2010-10-01

... handling, the request shall be prominently marked, both on the envelope and on the face of the request...) the failure to state willingness to pay fees as high as are anticipated by the Department will delay...

21 CFR 900.11 - Requirements for certification.

Code of Federal Regulations, 2011 CFR

2011-04-01

... clinical images needed to complete the accreditation process. To apply for and receive a provisional... basis of an act described in 41 U.S.C. 263b(i)(1), no person who owned or operated that facility at the...

26 CFR 1.263(a)-3 - Amounts paid to improve tangible property.

Code of Federal Regulations, 2014 CFR

2014-04-01

.... In addition, the turbine contains a series of blades that cause the turbine to rotate when affected... turbine blades, as separate units of property because each of these components does not perform a discrete...

DOE and NREL to Host Meeting for Community

Science.gov Websites

and NREL to Host Meeting for Community For more information contact: e:mail: Public Affairs Golden Center, 15013 Denver West Parkway in Golden. To reach the Visitors Center, take Interstate 70 to exit 263

12 CFR 263.65 - Civil penalty inflation adjustments.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) Knowingly or recklessly false or misleading reports, inter alia—$1,375,000. (2) 12 U.S.C. 504, 505, 1817(j...)(5) against an institution or enterprise during any calendar year—$135,000. [73 FR 58032, Oct. 6...

12 CFR 225.6 - Penalties for violations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Act or any regulation or order issued under it, or for making a false entry in any book, report, or... be made in accordance with subpart C of the Board's Rules of Practice for Hearings (12 CFR part 263...

Synthesis, structure and electrical properties of Cu{sub 3.21}Ti{sub 1.16}Nb{sub 2.63}O{sub 12} and the CuO{sub x}-TiO{sub 2}-Nb{sub 2}O{sub 5} pseudoternary phase diagram

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reeves-McLaren, Nik, E-mail: n.reeves@sheffield.ac.uk; Ferrarelli, Matthew C.; Tung, Yuan-Wei

2011-07-15

Subsolidus phase relations in the CuO{sub x}-TiO{sub 2}-Nb{sub 2}O{sub 5} system were determined at 935 deg. C. The phase diagram contains one new phase, Cu{sub 3.21}Ti{sub 1.16}Nb{sub 2.63}O{sub 12} (CTNO) and one rutile-structured solid solution series, Ti{sub 1-3x}Cu{sub x}Nb{sub 2x}O{sub 2}: 0 Subsolidus phase relations in the CuO{sub x}-TiO{sub 2}-Nb{sub 2}O{sub 5} system were determined at 935 deg. C. > A new phase, Cu{sub 3.21}Ti{sub 1.16}Nb{sub 2.63}O{sub 12} (CTNO), was found with a CaCu{sub 3}Ti{sub 4}O{sub 12}-like crystal structure. > We discovered one rutile-structured solid solution series, Ti{sub 1-3x}Cu{sub x}Nb{sub 2x}O{sub 2}: 0 CTNO has square planar Cu{sup 2+}, A site vacancies and Cu{sup +}, Ti{sup 4+} and Nb{sup 5+} disordered on octahedral sites. > CTNO is a modest semiconductor with relative permittivity {approx}63.« less

33 CFR 263.15 - Program policies.

Code of Federal Regulations, 2013 CFR

2013-07-01

... undertaken, without regard to time. (b) Applicability of costs to Federal and non-Federal shares. Unless... Corps of Engineers costs for investigations, design, and construction (including costs of supervision... costs of investigations, planning, design and construction, to include those incurred prior to...

33 CFR 263.15 - Program policies.

Code of Federal Regulations, 2012 CFR

2012-07-01

... undertaken, without regard to time. (b) Applicability of costs to Federal and non-Federal shares. Unless... Corps of Engineers costs for investigations, design, and construction (including costs of supervision... costs of investigations, planning, design and construction, to include those incurred prior to...

12 CFR 263.94 - Conduct warranting sanctions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... an attorney, or debarment or suspension from practice as a certified public accountant or public... that individual's period of suspension, debarment, or ineligibility; (f) Contemptuous conduct in... circulating or publishing malicious or libelous matter; (g) Suspension or debarment from practice before the...

Genetics Home Reference: glucose phosphate isomerase deficiency

MedlinePlus

... patient homozygous for the L487F mutation in the human GPI gene. Int J Hematol. 2012 Aug;96(2):263- ... PubMed Xu W, Beutler E. The characterization of gene mutations for human glucose phosphate isomerase deficiency associated with chronic hemolytic ...

26 CFR 1.263(a)-3T - Amounts paid to improve tangible property (temporary).

Code of Federal Regulations, 2013 CFR

2013-04-01

... electrical energy. In addition, the turbine contains a series of blades that cause the turbine to rotate when... is not required to treat components, such as the turbine blades, as separate units of property...

26 CFR 1.263(a)-3T - Amounts paid to improve tangible property (temporary).

Code of Federal Regulations, 2012 CFR

2012-04-01

... electrical energy. In addition, the turbine contains a series of blades that cause the turbine to rotate when... is not required to treat components, such as the turbine blades, as separate units of property...

78 FR 1570 - Semiannual Regulatory Agenda

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-08

... Transformers (energy efficiency standards) Residential clothes washers (energy efficiency standards... Distribution Transformers (Reg Plan Seq No. 32). 263 Test Procedures for 1904-AC76 Residential Refrigerators... Efficiency Standards for Distribution Transformers Regulatory Plan: This entry is Seq. No. 32 in part II of...

26 CFR 1.263(a)-1 - Capital expenditures; In general.

Code of Federal Regulations, 2010 CFR

2010-04-01

... amount paid out for new buildings or for permanent improvements or betterments made to increase the value... experimentation, (3) Section 175 and §§ 1.175-1 through 1.175-6, relating to soil and water conservation, (4...

26 CFR 1.263(a)-1 - Capital expenditures; In general.

Code of Federal Regulations, 2011 CFR

2011-04-01

... for: (1) Any amount paid out for new buildings or for permanent improvements or betterments made to... experimentation, (3) Section 175 and §§ 1.175-1 through 1.175-6, relating to soil and water conservation, (4...

49 CFR 173.121 - Class 3-Assignment of packing group.

Code of Federal Regulations, 2013 CFR

2013-10-01

... than 100 L (26.3 gallons); and (iv) The viscosity and flash point are in accordance with the following... paragraph (b)(1) of this section shall be performed are as follows: (i) Viscosity test. The flow time in...

49 CFR 173.121 - Class 3-Assignment of packing group.

Code of Federal Regulations, 2014 CFR

2014-10-01

... than 100 L (26.3 gallons); and (iv) The viscosity and flash point are in accordance with the following... paragraph (b)(1) of this section shall be performed are as follows: (i) Viscosity test. The flow time in...

75 FR 57020 - Proposed Agency Information Collection Activities: Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-17

... may call 202-263-4869. SUPPLEMENTARY INFORMATION: Proposal to extend for three years with revision the... collections are mandatory: 12 U.S.C. 3105(c)(2), 1817(a)(1) and (3), and 3102(b). Except for select sensitive...

Advancing the Science of Team Science

PubMed Central

Falk‐Krzesinski, Holly J.; Börner, Katy; Contractor, Noshir; Fiore, Stephen M.; Hall, Kara L.; Keyton, Joann; Spring, Bonnie; Stokols, Daniel; Trochim, William; Uzzi, Brian

2010-01-01

Abstract The First Annual International Science of Team Science (SciTS) Conference was held in Chicago, IL April 22–24, 2010. This article presents a summary of the Conference proceedings. Clin Trans Sci 2010; Volume 3: 263–266. PMID:20973925

12 CFR 263.401 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... be performed by an independent public accountant by section 36 of the FDIA and 12 CFR part 363... insured subsidiary bank of that holding company. (d) Independent public accountant (accountant) means any... PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From Performing Audit Services...

TANZANIA: Toward 20 Years of Unity.

DTIC Science & Technology

1983-06-01

mainland and Zanzibar. The principal cash crops are cashew nuts , coffee, sisal, sugar, tea, tobacco, and various spices. These agricul- tural products...coffee (33.8 percent), cotton (26.3 percent), and cashew nuts (22 percent). It recommended that the Tanzanian Government limit expenditures on less

12 CFR 263.402 - Removal, suspension, or debarment.

Code of Federal Regulations, 2011 CFR

2011-01-01

... recklessly engaged in conduct that results in a violation of applicable professional standards, including... Company Accounting Oversight Board and the Securities and Exchange Commission; (iii) Has engaged in... violation of applicable professional standards in circumstances in which an accountant knows, or should know...

12 CFR 263.402 - Removal, suspension, or debarment.

Code of Federal Regulations, 2014 CFR

2014-01-01

... recklessly engaged in conduct that results in a violation of applicable professional standards, including... Company Accounting Oversight Board and the Securities and Exchange Commission; (iii) Has engaged in... violation of applicable professional standards in circumstances in which an accountant knows, or should know...

12 CFR 263.402 - Removal, suspension, or debarment.

Code of Federal Regulations, 2012 CFR

2012-01-01

... recklessly engaged in conduct that results in a violation of applicable professional standards, including... Company Accounting Oversight Board and the Securities and Exchange Commission; (iii) Has engaged in... violation of applicable professional standards in circumstances in which an accountant knows, or should know...

12 CFR 263.402 - Removal, suspension, or debarment.

Code of Federal Regulations, 2013 CFR

2013-01-01

... recklessly engaged in conduct that results in a violation of applicable professional standards, including... Company Accounting Oversight Board and the Securities and Exchange Commission; (iii) Has engaged in... violation of applicable professional standards in circumstances in which an accountant knows, or should know...

Utilization of an Academic Nursing Center.

ERIC Educational Resources Information Center

Cole, Frank L.; Mackey, Thomas

1996-01-01

Using data from an academic nursing center that cared for 3,263 patients over eight months, diseases were classified using International Classification of Diseases codes, and procedures were classified using Current Procedural Terminology codes. Patterns of health care emerged, with implications for clinical teaching. (SK)

41 CFR 101-26.301 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Section 101-26.301 Public Contracts and Property Management Federal Property Management Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 26-PROCUREMENT SOURCES AND PROGRAM 26.3-Procurement of GSA Stock Items § 101-26.301 Applicability. All executive agencies within the United States...

29 CFR 1952.263 - Compliance staffing benchmarks.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... State operating an approved State plan. In 1992, Michigan completed, in conjunction with OSHA, a reassessment of the levels initially established in 1980 and proposed revised benchmarks of 56 safety and 45...

29 CFR 1952.263 - Compliance staffing benchmarks.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... State operating an approved State plan. In 1992, Michigan completed, in conjunction with OSHA, a reassessment of the levels initially established in 1980 and proposed revised benchmarks of 56 safety and 45...

40 CFR 52.263 - Priority treatment for buses and carpools-Los Angeles Region.

Code of Federal Regulations, 2012 CFR

2012-07-01

... distinctive painted, pylon, or physical barriers. (4) Vehicles using a bus/carpool lane shall have the right of way when crossing other portions of the road to enter or leave such lanes. (5) At a minimum, the...

40 CFR 52.263 - Priority treatment for buses and carpools-Los Angeles Region.

Code of Federal Regulations, 2014 CFR

2014-07-01

... distinctive painted, pylon, or physical barriers. (4) Vehicles using a bus/carpool lane shall have the right of way when crossing other portions of the road to enter or leave such lanes. (5) At a minimum, the...

40 CFR 52.263 - Priority treatment for buses and carpools-Los Angeles Region.

Code of Federal Regulations, 2010 CFR

2010-07-01

... distinctive painted, pylon, or physical barriers. (4) Vehicles using a bus/carpool lane shall have the right of way when crossing other portions of the road to enter or leave such lanes. (5) At a minimum, the...

40 CFR 52.263 - Priority treatment for buses and carpools-Los Angeles Region.

Code of Federal Regulations, 2013 CFR

2013-07-01

... distinctive painted, pylon, or physical barriers. (4) Vehicles using a bus/carpool lane shall have the right of way when crossing other portions of the road to enter or leave such lanes. (5) At a minimum, the...

40 CFR 52.263 - Priority treatment for buses and carpools-Los Angeles Region.

Code of Federal Regulations, 2011 CFR

2011-07-01

... distinctive painted, pylon, or physical barriers. (4) Vehicles using a bus/carpool lane shall have the right of way when crossing other portions of the road to enter or leave such lanes. (5) At a minimum, the...

34 CFR 263.8 - What are the payback requirements?

Code of Federal Regulations, 2011 CFR

2011-07-01

... EDUCATION, DEPARTMENT OF EDUCATION INDIAN EDUCATION DISCRETIONARY GRANT PROGRAMS Professional Development... Professional Development program are required to— (1) Sign an agreement, at the time of selection for training... for which training was actually received under the Professional Development program. (c) The cash...

77 FR 4708 - Proposed Amendment of Class E Airspace; Tobe, CO

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-31

.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 [Amended] 2. The incorporation by reference... the southeast by V-263, and on the west by V-389. Issued in Seattle, Washington, on January 23, 2012...

76 FR 13122 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-10

... Malinowski, Southeast Regional Office, NMFS, 263 13th Avenue, South, St. Petersburg, FL 33701. Instructions... INFORMATION CONTACT: Rich Malinowski, 727-824-5305; fax: 727-824-5308. SUPPLEMENTARY INFORMATION: The reef...

50 CFR 600.502 - Vessel reports.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., National Marine Fisheries Service, NOAA, One Blackburn Drive, Gloucester, MA 01930-2298 Director, Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, 166 Water St., Woods Hole, MA 02543-1097... Region, National Marine Fisheries Service, 263 13th Ave. South, St. Petersburg, FL 33701 Director...

50 CFR 600.502 - Vessel reports.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., National Marine Fisheries Service, NOAA, One Blackburn Drive, Gloucester, MA 01930-2298 Director, Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, 166 Water St., Woods Hole, MA 02543-1097... Region, National Marine Fisheries Service, 263 13th Ave. South, St. Petersburg, FL 33701 Director...

50 CFR 600.502 - Vessel reports.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., National Marine Fisheries Service, NOAA, One Blackburn Drive, Gloucester, MA 01930-2298 Director, Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, 166 Water St., Woods Hole, MA 02543-1097... Region, National Marine Fisheries Service, 263 13th Ave. South, St. Petersburg, FL 33701 Director...

12 CFR 263.401 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

...) RULES OF PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From Performing Audit... be performed by an independent public accountant by section 36 of the FDIA and 12 CFR part 363... company. (d) Independent public accountant (accountant) means any individual who performs or participates...

12 CFR 263.401 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From Performing Audit Services... be performed by an independent public accountant by section 36 of the FDIA and 12 CFR part 363... insured subsidiary bank of that holding company. (d) Independent public accountant (accountant) means any...

12 CFR 263.401 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

...) RULES OF PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From Performing Audit... be performed by an independent public accountant by section 36 of the FDIA and 12 CFR part 363... company. (d) Independent public accountant (accountant) means any individual who performs or participates...

12 CFR 263.401 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

...) RULES OF PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From Performing Audit... be performed by an independent public accountant by section 36 of the FDIA and 12 CFR part 363... company. (d) Independent public accountant (accountant) means any individual who performs or participates...

Structure-5-HT/D2 Receptor Affinity Relationship in a New Group of 1-Arylpiperazynylalkyl Derivatives of 8-Dialkylamino-3,7-dimethyl-1H-purine-2,6(3H,7H)-dione.

PubMed

Żmudzki, Paweł; Satała, Grzegorz; Chłoń-Rzepa, Grażyna; Bojarski, Andrzej J; Kazek, Grzegorz; Siwek, Agata; Gryboś, Anna; Głuch-Lutwin, Monika; Wesołowska, Anna; Pawłowski, Maciej

2016-10-01

In our previous papers, we have reported that some 8-amino-1,3-dimethyl-1H-purine-2,6(3H,7H)-dione derivatives possessed high affinity and displayed agonistic, partial agonistic, or antagonistic activity for serotonin 5-HT 1A and dopamine D 2 receptors. In order to examine further the influence of the substituent in the position 8 of the purine moiety and the influence of the xanthine core on the affinity for serotonin 5-HT 1A , 5-HT 2A , 5-HT 6 , 5-HT 7 , and dopamine D 2 receptors, two series of 1-arylpiperazynylalkyl derivatives of 8-amino-3,7-dimethyl-1H-purine-2,6(3H,7H)-dione were synthesized. All the final compounds were investigated in in vitro competition binding experiments for the serotonin 5-HT 1A , 5-HT 2A , 5-HT 6 , 5-HT 7 , and dopamine D 2 receptors. The structure-affinity relationships for this group of compounds were discussed. For selected compounds, the functional assays for the 5-HT 1A and D 2 receptors were carried out. The results of the assays indicated that these groups of derivatives possessed antagonistic activity for 5-HT 1A receptors and agonistic, partial agonistic, or antagonistic activity for D 2 receptors. In total, 26 new compounds were synthesized, 20 of which were tested in in vitro binding experiments and 5 were tested in in vitro functional assays. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Metabolic reprogramming of glioblastoma cells by L-asparaginase sensitizes for apoptosis in vitro and in vivo

PubMed Central

Karpel-Massler, Georg; Ramani, Doruntina; Shu, Chang; Halatsch, Marc-Eric; Westhoff, Mike-Andrew; Bruce, Jeffrey N.; Canoll, Peter; Siegelin, Markus D.

2016-01-01

Cancer cells display a variety of global metabolic changes, which aside from the glycolytic pathway largely involve amino acid metabolism. To ensure aggressive growth, tumor cells highly depend on amino acids, most notably due to their pivotal need of protein synthesis. In this study, we assessed the overall hypothesis that depletion of asparagine by E. coli-derived L-asparaginase might be a novel means for the therapy of one of the most recalcitrant neoplasms and for which no efficient treatment currently exists - glioblastoma (WHO grade IV). Our results suggest that certain glioma cell cultures are particularly susceptible to inhibition of proliferation by L-asparaginase, while others display a more resistant phenotype. In sensitive cells, L-asparaginase induces apoptosis with dissipation of mitochondrial membrane potential and activation of effector caspases. L-asparaginase-mediated apoptosis was accompanied by modulation of pro- and anti-apoptotic Bcl-2 family members, including Noxa, Mcl-1 and the deubiquitinase Usp9X. Given the impact of L-asparaginase on these molecules, we found that L-asparaginase potently overcomes resistance to both intrinsic apoptosis induced by the Bcl-2/Bcl-xL inhibitor, ABT263, and extrinsic apoptosis mediated by TRAIL even in glioma cells that are resistant towards L-asparaginase single treatment. RNA interference studies showed that Usp9X, Mcl-1, Noxa and Bax/Bak are involved in ABT263/L-asparaginase-mediated cell death. In vivo, combined treatment with ABT263 and L-asparaginase led to an enhanced reduction of tumor growth when compared to each reagent alone without induction of toxicity. These observations suggest that L-asparaginase might be useful for the treatment of malignant glial neoplasms. PMID:27172899

Zygotic and somatic embryo morphogenesis in Pinus pinaster: comparative histological and histochemical study.

PubMed

Tereso, Susana; Zoglauer, Kurt; Milhinhos, Ana; Miguel, Célia; Oliveira, M Margarida

2007-05-01

We compared morphogenesis and accumulation of storage proteins and starch in Pinus pinaster Ait. zygotic embryos with those in somatic embryos grown with different carbohydrate sources. The maturation medium for somatic embryos included 80 microM abscisic acid (ABA), 9 g l(-1) gellam gum and either glucose, sucrose or maltose at 44, 88, 175 or 263 mM in the presence or absence of 6% (w/v) polyethylene glycol (PEG) 4000 MW. Maturation medium containing 44 or 88 mM of a carbohydrate source produced only one or no cotyledonary somatic embryos per 0.6 g fresh mass of culture. The addition of PEG to the basal maturation medium resulted in a low yield of cotyledonary somatic embryos that generally showed incomplete development and anatomical abnormalities such as large intercellular spaces and large vacuoles. High concentrations of maltose also induced large intercellular spaces in the somatic embryonic cells, and 263 mM sucrose produced fewer and less developed cotyledonary somatic embryos compared with 175 mM sucrose, indicating that the effect of carbohydrate source is partially osmotic. Zygotic embryos had a lower dry mass than somatic embryos at the same stage of development. Starch granules followed a similar accumulation pattern in zygotic and somatic embryos. A low starch content was found in cotyledonary zygotic embryos and in somatic embryos developed in the presence of 175 mM maltose or 263 mM glucose. In zygotic embryos and in PEG-treated somatic embryos, protein bodies appeared later and were smaller and fewer than in well-developed somatic embryos grown without PEG. We propose that storage protein concentration might be a marker of embryo quality.

Extended cold snare polypectomy for small colorectal polyps increases the R0 resection rate.

PubMed

Abe, Yasuhiro; Nabeta, Haruaki; Koyanagi, Ryota; Nakamichi, Taro; Hirashima, Hayato; Lefor, Alan Kawarai; Shinozaki, Satoshi

2018-02-01

 Despite widespread use of cold snare polypectomy (CSP), the R0 resection rate is not well documented. We perform extended CSP, resecting polyps with a > 1 mm circumferential margin. The aim of this study is to compare the R0 resection rate of extended CSP with conventional CSP and to assess safety. From April 2014 to September 2016, 712 non-pedunculated colorectal polyps, < 10 mm in size, resected using CSP from 316 patients were retrospectively analyzed.  We divided lesions into conventional CSP (n = 263) and extended CSP groups (n = 449). The baseline characteristics of these two groups were not significantly different in univariate or multivariate analyses. Sessile polyps comprised 94 % (668/712), and the remaining were flat-elevated polyps. Mean size of polyps (±standard deviation) was 4.2 ± 1.5 mm. The most frequent pathology was low grade adenoma (97 %, 689/712). The R0 resection rate was significantly higher in the extended CSP group (439/449 [98 %]) than in the conventional CSP group (222/263 [84 %], P  < 0.001). There was no delayed bleeding or perforation in either group (conventional CSP group, 0/263, 95 % confidence interval: 0.0 - 1.4 % and extended CSP group, 0/449, 95 % confidence interval: 0.0 - 0.8 %).  Extended CSP results in a higher R0 resection rate compared with conventional CSP. Extended CSP did not result in a higher rate of delayed bleeding or perforation. Extended CSP is a safe and promising procedure for endoscopic resection of non-pedunculated colorectal polyps < 10 mm in size.

Scrapie Agent (Strain 263K) Can Transmit Disease via the Oral Route after Persistence in Soil over Years

PubMed Central

Groschup, Martin H.; Peters, Rainer; Beekes, Michael; Terytze, Konstantin

2007-01-01

The persistence of infectious biomolecules in soil constitutes a substantial challenge. This holds particularly true with respect to prions, the causative agents of transmissible spongiform encephalopathies (TSEs) such as scrapie, bovine spongiform encephalopathy (BSE), or chronic wasting disease (CWD). Various studies have indicated that prions are able to persist in soil for years without losing their pathogenic activity. Dissemination of prions into the environment can occur from several sources, e.g., infectious placenta or amniotic fluid of sheep. Furthermore, environmental contamination by saliva, excrements or non-sterilized agricultural organic fertilizer is conceivable. Natural transmission of scrapie in the field seems to occur via the alimentary tract in the majority of cases, and scrapie-free sheep flocks can become infected on pastures where outbreaks of scrapie had been observed before. These findings point to a sustained contagion in the environment, and notably the soil. By using outdoor lysimeters, we simulated a contamination of standard soil with hamster-adapted 263K scrapie prions, and analyzed the presence and biological activity of the soil-associated PrPSc and infectivity by Western blotting and hamster bioassay, respectively. Our results showed that 263K scrapie agent can persist in soil at least over 29 months. Strikingly, not only the contaminated soil itself retained high levels of infectivity, as evidenced by oral administration to Syrian hamsters, but also feeding of aqueous soil extracts was able to induce disease in the reporter animals. We could also demonstrate that PrPSc in soil, extracted after 21 months, provides a catalytically active seed in the protein misfolding cyclic amplification (PMCA) reaction. PMCA opens therefore a perspective for considerably improving the detectability of prions in soil samples from the field. PMID:17502917

Mutations close to a hub residue affect the distant active site of a GH1 β-glucosidase.

PubMed

Souza, Valquiria P; Ikegami, Cecília M; Arantes, Guilherme M; Marana, Sandro R

2018-01-01

The tertiary structure of proteins has been represented as a network, in which residues are nodes and their contacts are edges. Protein structure networks contain residues, called hubs or central, which are essential to form short connection pathways between any pair of nodes. Hence hub residues may effectively spread structural perturbations through the protein. To test whether modifications nearby to hub residues could affect the enzyme active site, mutations were introduced in the β-glycosidase Sfβgly (PDB-ID: 5CG0) directed to residues that form an α-helix (260-265) and a β-strand (335-337) close to one of its main hub residues, F251, which is approximately 14 Å from the Sfβgly active site. Replacement of residues A263 and A264, which side-chains project from the α-helix towards F251, decreased the rate of substrate hydrolysis. Mutation A263F was shown to weaken noncovalent interactions involved in transition state stabilization within the Sfβgly active site. Mutations placed on the opposite side of the same α-helix did not show these effects. Consistently, replacement of V336, which side-chain protrudes from a β-strand face towards F251, inactivated Sfβgly. Next to V336, mutation S337F also caused a decrease in noncovalent interactions involved in transition state stabilization. Therefore, we suggest that mutations A263F, A264F, V336F and S337F may directly perturb the position of the hub F251, which could propagate these perturbations into the Sfβgly active site through short connection pathways along the protein network.

Structural modulation of factor VIIa by full-length tissue factor (TF1-263): implication of novel interactions between EGF2 domain and TF.

PubMed

Prasad, Ramesh; Sen, Prosenjit

2018-02-01

Tissue factor (TF)-mediated factor VII (FVII) activation and a subsequent proteolytic TF-FVIIa binary complex formation is the key step initiating the coagulation cascade, with implications in various homeostatic and pathologic scenarios. TF binding allosterically modifies zymogen-like free FVIIa to its highly catalytically active form. As a result of unresolved crystal structure of the full-length TF 1-263 -FVIIa binary complex and free FVIIa, allosteric alterations in FVIIa following its binding to full-length TF and the consequences of these on function are not entirely clear. The present study aims to map and identify structural alterations in FVIIa and TF resulting from full-length TF binding to FVIIa and the key events responsible for enhanced FVIIa activity in coagulation. We constructed the full-length TF 1-263 -FVIIa membrane bound complex using computational modeling and subjected it to molecular dynamics (MD) simulations. MD simulations showed that TF alters the structure of each domain of FVIIa and these combined alterations contribute to enhanced TF-FVIIa activity. Detailed, domain-wise investigation revealed several new non-covalent interactions between TF and FVIIa that were not found in the truncated soluble TF-FVIIa crystal structure. The structural modulation of each FVIIa domain imparted by TF indicated that both inter and intra-domain communication is crucial for allosteric modulation of FVIIa. Our results suggest that these newly formed interactions can provide additional stability to the protease domain and regulate its activity profile by governing catalytic triad (CT) orientation and localization. The unexplored newly formed interactions between EGF2 and TF provides a possible explanation for TF-induced allosteric activation of FVIIa.

Direct on-strip analysis of size- and time-resolved aerosol impactor samples using laser induced fluorescence spectra excited at 263 and 351 nm.

PubMed

Wang, Chuji; Pan, Yong-Le; James, Deryck; Wetmore, Alan E; Redding, Brandon

2014-04-11

We report a novel atmospheric aerosol characterization technique, in which dual wavelength UV laser induced fluorescence (LIF) spectrometry marries an eight-stage rotating drum impactor (RDI), namely UV-LIF-RDI, to achieve size- and time-resolved analysis of aerosol particles on-strip. The UV-LIF-RDI technique measured LIF spectra via direct laser beam illumination onto the particles that were impacted on a RDI strip with a spatial resolution of 1.2mm, equivalent to an averaged time resolution in the aerosol sampling of 3.6 h. Excited by a 263 nm or 351 nm laser, more than 2000 LIF spectra within a 3-week aerosol collection time period were obtained from the eight individual RDI strips that collected particles in eight different sizes ranging from 0.09 to 10 μm in Djibouti. Based on the known fluorescence database from atmospheric aerosols in the US, the LIF spectra obtained from the Djibouti aerosol samples were found to be dominated by fluorescence clusters 2, 5, and 8 (peaked at 330, 370, and 475 nm) when excited at 263 nm and by fluorescence clusters 1, 2, 5, and 6 (peaked at 390 and 460 nm) when excited at 351 nm. Size- and time-dependent variations of the fluorescence spectra revealed some size and time evolution behavior of organic and biological aerosols from the atmosphere in Djibouti. Moreover, this analytical technique could locate the possible sources and chemical compositions contributing to these fluorescence clusters. Advantages, limitations, and future developments of this new aerosol analysis technique are also discussed. Published by Elsevier B.V.

Somatic GPR101 Duplication Causing X-Linked Acrogigantism (XLAG)-Diagnosis and Management.

PubMed

Rodd, Celia; Millette, Maude; Iacovazzo, Donato; Stiles, Craig E; Barry, Sayka; Evanson, Jane; Albrecht, Steffen; Caswell, Richard; Bunce, Benjamin; Jose, Sian; Trouillas, Jacqueline; Roncaroli, Federico; Sampson, Julian; Ellard, Sian; Korbonits, Márta

2016-05-01

Recent reports have proposed that sporadic or familial germline Xq26.3 microduplications involving the GPR101 gene are associated with early-onset X-linked acrogigantism (XLAG) with a female preponderance. A 4-year-old boy presented with rapid growth over the previous 2 years. He complained of sporadic headaches and had coarse facial features. His height Z-score was +4.89, and weight Z-score was +5.57. Laboratory testing revealed elevated serum prolactin (185 μg/L; normal, <18 μg/L), IGF-1 (745 μg/L; normal, 64-369 μg/L), and fasting GH > 35.0 μg/L. Magnetic resonance imaging demonstrated a homogenous bulky pituitary gland (18 × 15 × 13 mm) without obvious adenoma. A pituitary biopsy showed hyperplastic pituitary tissue with enlarged cords of GH and prolactin cells. Germline PRKAR1A, MEN1, AIP, DICER1, CDKN1B, and somatic GNAS mutations were negative. Medical management was challenging until institution of continuous sc infusion of short-acting octreotide combined with sc pegvisomant and oral cabergoline. The patient remains well controlled with minimal side effects 7 years after presentation. His phenotype suggested XLAG, but his peripheral leukocyte-, saliva-, and buccal cell-derived DNA tested negative for microduplication in Xq26.3 or GPR101. However, DNA isolated from the pituitary tissue and forearm skin showed duplicated dosage of GPR101, suggesting that he is mosaic for this genetic abnormality. Our patient is the first to be described with somatic microduplication leading to typical XLAG phenotype. This patient demonstrates that a negative test for Xq26.3 microduplication or GPR101 duplication on peripheral blood DNA does not exclude the diagnosis of XLAG because it can result from a mosaic mutation affecting the pituitary.

Randomised controlled trials of homeopathy in humans: characterising the research journal literature for systematic review.

PubMed

Mathie, Robert T; Hacke, Daniela; Clausen, Jürgen; Nicolai, Ton; Riley, David S; Fisher, Peter

2013-01-01

A new programme of systematic reviews of randomised controlled trials (RCTs) in homeopathy will distinguish important attributes of RCT records, including: placebo controlled versus other-than-placebo (OTP) controlled; individualised versus non-individualised homeopathy; peer-reviewed (PR) versus non peer-reviewed (NPR) sources. (a) To outline the methods used to search and categorise the RCT literature; (b) to report details of the records retrieved; (c) to compare our retrieved records with those reported in two previous systematic reviews (Linde et al., 1997; Shang et al., 2005). Ten major electronic databases were searched for records published up to the end of 2011. A record was accepted for subsequent systematic review if it was a substantive report of a clinical trial of homeopathic treatment or prophylaxis in humans, randomised and controlled, and published in a PR or NPR journal. 489 records were potentially eligible: 226 were rejected as non-journal, minor or repeat publications, or lacking randomisation and/or controls and/or a 'homeopathic' intervention; 263 (164 PR, 99 NPR) were acceptable for systematic review. The 263 accepted records comprised 217 (137 PR, 80 NPR) placebo-controlled RCTs, of which 121 were included by, 66 were published after, and 30 were potentially eligible for, but not listed by, Linde or Shang. The 137 PR records of placebo-controlled RCTs comprise 41 on individualised homeopathy and 96 on non-individualised homeopathy. Our findings clarify the RCT literature in homeopathy. The 263 accepted journal papers will be the basis for our forthcoming programme of systematic reviews. Copyright © 2012 The Faculty of Homeopathy. Published by Elsevier Ltd. All rights reserved.