Sample records for bohrium 264

  1. Road dust and its effect on human health: a literature review

    PubMed Central

    2018-01-01

    The purpose of this study was to determine the effects of road dust on human health. A PubMed search was used to extract references that included the words “road dust” and “health” or “fugitive dust” and “health” in the title or abstract. A total of 46 references were extracted and selected for review after the primary screening of 949 articles. The respiratory system was found to be the most affected system in the human body. Lead, platinum-group elements (platinum, rhodium, and bohrium), aluminum, zinc, vanadium, and polycyclic aromatic hydrocarbons were the components of road dust that were most frequently referenced in the articles reviewed. Road dust was found to have harmful effects on the human body, especially on the respiratory system. To determine the complex mechanism of action of various components of road dust on the human body and the results thereof, the authors recommend a further meta-analysis and extensive risk-assessment research into the health impacts of dust exposure. PMID:29642653

  2. Comparative Effect of an Addition of a Surface Term to Woods-Saxon Potential on Thermodynamics of a Nucleon

    NASA Astrophysics Data System (ADS)

    Lütfüoğlu, B. C.

    2018-01-01

    In this study, we reveal the difference between Woods-Saxon (WS) and Generalized Symmetric Woods-Saxon (GSWS) potentials in order to describe the physical properties of a nucleon, by means of solving Schrödinger equation for the two potentials. The additional term squeezes the WS potential well, which leads an upward shift in the spectrum, resulting in a more realistic picture. The resulting GSWS potential does not merely accommodate extra quasi bound states, but also has modified bound state spectrum. As an application, we apply the formalism to a real problem, an α particle confined in Bohrium-270 nucleus. The thermodynamic functions Helmholtz energy, entropy, internal energy, specific heat of the system are calculated and compared for both wells. The internal energy and the specific heat capacity increase as a result of upward shift in the spectrum. The shift of the Helmholtz free energy is a direct consequence of the shift of the spectrum. The entropy decreases because of a decrement in the number of available states. Supported by the Turkish Science and Research Council (TÜBİTAK) and Akdeniz University

  3. Impact of a potential glycosylation site at neuraminidase amino acid 264 of influenza A/H9N2 virus.

    PubMed

    Shao, Hongxia; Zhou, Xiaoxiang; Fan, Zhonglei; Wan, Zhimin; Qian, Kun; Perez, Daniel; Qin, Aijian; Ye, Jianqiang

    2016-11-30

    To determine the role of the potential glycosylation site NA264N, which has been shown to be prevalent in recent Chinese H9N2 isolates, four reverse genetic viruses, rgWS1-NA264N, rgWS1-NA264H, rgBJ-NA264H and rgBJ-NA264N, were rescued. Growth kinetics showed that viruses with NA264H grew faster than viruses with NA264N. Mouse studies revealed that rgBJ-NA264H replicated to a significantly higher titer than rgBJ-NA264N at 3dpi. Notably, in contact chickens, rgBJ-NA264H and rgWS1-NA264H shed significantly more virus than rgBJ-NA264N at 6dpi from the larynx and rgWS1-NA264N at 4dpi from the cloaca, respectively. The present study demonstrates that NA264N affects viral replication of H9N2. Copyright © 2016 Elsevier B.V. All rights reserved.

  4. 40 CFR 264.1065 - Reporting requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... identification number of each pump for which a leak was not repaired as required in § 264.1052 (c) and (d)(6... control device installed as required by § 264.1052, 264.1053, 264.1054, or 264.1055 exceeded or operated outside of the design specifications as defined in § 264.1064(e) and as indicated by the control device...

  5. 40 CFR 264.1065 - Reporting requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... identification number of each pump for which a leak was not repaired as required in § 264.1052 (c) and (d)(6... control device installed as required by § 264.1052, 264.1053, 264.1054, or 264.1055 exceeded or operated outside of the design specifications as defined in § 264.1064(e) and as indicated by the control device...

  6. 40 CFR 264.1065 - Reporting requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... identification number of each pump for which a leak was not repaired as required in § 264.1052 (c) and (d)(6... control device installed as required by § 264.1052, 264.1053, 264.1054, or 264.1055 exceeded or operated outside of the design specifications as defined in § 264.1064(e) and as indicated by the control device...

  7. 40 CFR 264.1065 - Reporting requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... identification number of each pump for which a leak was not repaired as required in § 264.1052 (c) and (d)(6... control device installed as required by § 264.1052, 264.1053, 264.1054, or 264.1055 exceeded or operated outside of the design specifications as defined in § 264.1064(e) and as indicated by the control device...

  8. 40 CFR 264.1065 - Reporting requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... identification number of each pump for which a leak was not repaired as required in § 264.1052 (c) and (d)(6... control device installed as required by § 264.1052, 264.1053, 264.1054, or 264.1055 exceeded or operated outside of the design specifications as defined in § 264.1064(e) and as indicated by the control device...

  9. 40 CFR 264.224-264.225 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false [Reserved] 264.224-264.225 Section 264.224-264.225 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  10. 40 CFR 264.274-264.275 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....274-264.275 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment §§ 264.274-264.275 [Reserved] ...

  11. 40 CFR 264.274-264.275 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....274-264.275 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment §§ 264.274-264.275 [Reserved] ...

  12. 40 CFR 264.274-264.275 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....274-264.275 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment §§ 264.274-264.275 [Reserved] ...

  13. 40 CFR 264.274-264.275 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....274-264.275 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment §§ 264.274-264.275 [Reserved] ...

  14. 40 CFR 264.274-264.275 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....274-264.275 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment §§ 264.274-264.275 [Reserved] ...

  15. 40 CFR 264.117 - Post-closure care and use of property.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... 264.117 Section 264.117 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... for each hazardous waste management unit subject to the requirements of §§ 264.117 through 264.120... preceding partial closure of a hazardous waste management unit subject to post-closure care requirements or...

  16. 40 CFR 264.52 - Content of contingency plan.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Content of contingency plan. 264.52... Contingency Plan and Emergency Procedures § 264.52 Content of contingency plan. (a) The contingency plan must describe the actions facility personnel must take to comply with §§ 264.51 and 264.56 in response to fires...

  17. 40 CFR 264.52 - Content of contingency plan.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Content of contingency plan. 264.52... Contingency Plan and Emergency Procedures § 264.52 Content of contingency plan. (a) The contingency plan must describe the actions facility personnel must take to comply with §§ 264.51 and 264.56 in response to fires...

  18. 40 CFR 264.52 - Content of contingency plan.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Content of contingency plan. 264.52... Contingency Plan and Emergency Procedures § 264.52 Content of contingency plan. (a) The contingency plan must describe the actions facility personnel must take to comply with §§ 264.51 and 264.56 in response to fires...

  19. 40 CFR 264.602 - Monitoring, analysis, inspection, response, reporting, and corrective action.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE....75, 264.76, 264.77, and -264.101 as well as meet any additional requirements needed to protect human...

  20. 40 CFR 264.602 - Monitoring, analysis, inspection, response, reporting, and corrective action.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE....75, 264.76, 264.77, and -264.101 as well as meet any additional requirements needed to protect human...

  1. 40 CFR 264.602 - Monitoring, analysis, inspection, response, reporting, and corrective action.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE....75, 264.76, 264.77, and -264.101 as well as meet any additional requirements needed to protect human...

  2. 40 CFR 264.602 - Monitoring, analysis, inspection, response, reporting, and corrective action.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE....75, 264.76, 264.77, and -264.101 as well as meet any additional requirements needed to protect human...

  3. 40 CFR 264.602 - Monitoring, analysis, inspection, response, reporting, and corrective action.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE....75, 264.76, 264.77, and -264.101 as well as meet any additional requirements needed to protect human...

  4. 40 CFR 264.258 - Closure and post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Waste Piles § 264.258 Closure and post-closure care. (a) At closure, the owner or operator must remove... that apply to landfills (§ 264.310). (c)(1) The owner or operator of a waste pile that does not comply...(c) or § 264.251(b), must: (i) Include in the closure plan for the pile under § 264.112 both a plan...

  5. 40 CFR 264.230 - Special requirements for incompatible wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... wastes. 264.230 Section 264.230 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Surface Impoundments § 264.230 Special requirements for incompatible wastes...

  6. 40 CFR 264.255 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false [Reserved] 264.255 Section 264.255 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Waste Piles § 264.255...

  7. 40 CFR 264.2 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false [Reserved] 264.2 Section 264.2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES General § 264.2...

  8. 40 CFR 264.277 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false [Reserved] 264.277 Section 264.277 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264...

  9. 40 CFR 264.282 - Special requirements for incompatible wastes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... wastes. 264.282 Section 264.282 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.282 Special requirements for incompatible wastes. The owner or...

  10. 40 CFR 264.277 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false [Reserved] 264.277 Section 264.277 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264...

  11. 40 CFR 264.277 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false [Reserved] 264.277 Section 264.277 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264...

  12. 40 CFR 264.282 - Special requirements for incompatible wastes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... wastes. 264.282 Section 264.282 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.282 Special requirements for incompatible wastes. The owner or...

  13. 40 CFR 264.277 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false [Reserved] 264.277 Section 264.277 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264...

  14. 40 CFR 264.282 - Special requirements for incompatible wastes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... wastes. 264.282 Section 264.282 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.282 Special requirements for incompatible wastes. The owner or...

  15. 40 CFR 264.282 - Special requirements for incompatible wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... wastes. 264.282 Section 264.282 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.282 Special requirements for incompatible wastes. The owner or...

  16. 40 CFR 264.179 - Air emission standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Air emission standards. 264.179 Section 264.179 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Use and Management of Containers § 264.179 Air emission standards. The owner or operator shall manage...

  17. 40 CFR 264.232 - Air emission standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Air emission standards. 264.232 Section 264.232 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Surface Impoundments § 264.232 Air emission standards. The owner or operator shall manage all hazardous...

  18. 40 CFR 264.179 - Air emission standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Air emission standards. 264.179 Section 264.179 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Use and Management of Containers § 264.179 Air emission standards. The owner or operator shall manage...

  19. 40 CFR 264.200 - Air emission standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Air emission standards. 264.200 Section 264.200 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Tank Systems § 264.200 Air emission standards. The owner or operator shall manage all hazardous waste...

  20. 40 CFR 264.200 - Air emission standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Air emission standards. 264.200 Section 264.200 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Tank Systems § 264.200 Air emission standards. The owner or operator shall manage all hazardous waste...

  1. 40 CFR 264.232 - Air emission standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Air emission standards. 264.232 Section 264.232 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Surface Impoundments § 264.232 Air emission standards. The owner or operator shall manage all hazardous...

  2. 39 CFR 264.4 - Vital Records Program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Vital Records Program. 264.4 Section 264.4 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.4 Vital Records Program. Complete procedures concerning the identification, categorization, processing, protection, and...

  3. 40 CFR 264.232 - Air emission standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Air emission standards. 264.232 Section 264.232 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Surface Impoundments § 264.232 Air emission standards. The owner or operator shall manage all hazardous...

  4. 40 CFR 264.200 - Air emission standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Air emission standards. 264.200 Section 264.200 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Tank Systems § 264.200 Air emission standards. The owner or operator shall manage all hazardous waste...

  5. 40 CFR 264.179 - Air emission standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Air emission standards. 264.179 Section 264.179 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Use and Management of Containers § 264.179 Air emission standards. The owner or operator shall manage...

  6. 40 CFR 264.232 - Air emission standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Air emission standards. 264.232 Section 264.232 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Surface Impoundments § 264.232 Air emission standards. The owner or operator shall manage all hazardous...

  7. 40 CFR 264.200 - Air emission standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Air emission standards. 264.200 Section 264.200 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Tank Systems § 264.200 Air emission standards. The owner or operator shall manage all hazardous waste...

  8. 40 CFR 264.179 - Air emission standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Air emission standards. 264.179 Section 264.179 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Use and Management of Containers § 264.179 Air emission standards. The owner or operator shall manage...

  9. 39 CFR 264.3 - Responsibility.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 39 Postal Service 1 2013-07-01 2013-07-01 false Responsibility. 264.3 Section 264.3 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.3 Responsibility. (a) Manager, Records Office. The Manager, Records Office, is responsible for categorizing records as vital...

  10. 39 CFR 264.3 - Responsibility.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 39 Postal Service 1 2012-07-01 2012-07-01 false Responsibility. 264.3 Section 264.3 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.3 Responsibility. (a) Manager, Records Office. The Manager, Records Office, is responsible for categorizing records as vital...

  11. 39 CFR 264.3 - Responsibility.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 39 Postal Service 1 2011-07-01 2011-07-01 false Responsibility. 264.3 Section 264.3 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.3 Responsibility. (a) Manager, Records Office. The Manager, Records Office, is responsible for categorizing records as vital...

  12. 39 CFR 264.3 - Responsibility.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Responsibility. 264.3 Section 264.3 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.3 Responsibility. (a) Manager, Records Office. The Manager, Records Office, is responsible for categorizing records as vital...

  13. 39 CFR 264.3 - Responsibility.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 39 Postal Service 1 2014-07-01 2014-07-01 false Responsibility. 264.3 Section 264.3 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.3 Responsibility. (a) Manager, Records Office. The Manager, Records Office, is responsible for categorizing records as vital...

  14. 40 CFR 264.229 - Special requirements for ignitable or reactive waste.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... reactive waste. 264.229 Section 264.229 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Surface Impoundments § 264.229 Special requirements for ignitable or reactive...

  15. 40 CFR 264.281 - Special requirements for ignitable or reactive waste.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... reactive waste. 264.281 Section 264.281 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.281 Special requirements for ignitable or reactive waste...

  16. 40 CFR 264.281 - Special requirements for ignitable or reactive waste.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... reactive waste. 264.281 Section 264.281 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.281 Special requirements for ignitable or reactive waste...

  17. 40 CFR 264.281 - Special requirements for ignitable or reactive waste.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... reactive waste. 264.281 Section 264.281 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.281 Special requirements for ignitable or reactive waste...

  18. 40 CFR 264.281 - Special requirements for ignitable or reactive waste.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... reactive waste. 264.281 Section 264.281 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.281 Special requirements for ignitable or reactive waste...

  19. 36 CFR 264.12 - Use without permission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Use without permission. 264.12 Section 264.12 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE PROPERTY MANAGEMENT Mount St. Helens National Volcanic Monument Symbol § 264.12 Use without permission...

  20. 40 CFR 264.553 - Temporary Units (TU).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Temporary Units (TU). 264.553 Section 264.553 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... Provisions for Cleanup § 264.553 Temporary Units (TU). (a) For temporary tanks and container storage areas...

  1. 40 CFR 264.553 - Temporary Units (TU).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Temporary Units (TU). 264.553 Section 264.553 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... Provisions for Cleanup § 264.553 Temporary Units (TU). (a) For temporary tanks and container storage areas...

  2. 40 CFR 264.14 - Security.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Security. 264.14 Section 264.14... Standards § 264.14 Security. (a) The owner or operator must prevent the unknowing entry, and minimize the...) for discussion of security requirements at disposal facilities during the post-closure care period...

  3. 40 CFR 264.14 - Security.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Security. 264.14 Section 264.14... Standards § 264.14 Security. (a) The owner or operator must prevent the unknowing entry, and minimize the...) for discussion of security requirements at disposal facilities during the post-closure care period...

  4. 40 CFR 264.14 - Security.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Security. 264.14 Section 264.14... Standards § 264.14 Security. (a) The owner or operator must prevent the unknowing entry, and minimize the...) for discussion of security requirements at disposal facilities during the post-closure care period...

  5. 40 CFR 264.14 - Security.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Security. 264.14 Section 264.14... Standards § 264.14 Security. (a) The owner or operator must prevent the unknowing entry, and minimize the...) for discussion of security requirements at disposal facilities during the post-closure care period...

  6. 34 CFR 75.264 - Transfers among budget categories.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Transfers among budget categories. 75.264 Section 75.264 Education Office of the Secretary, Department of Education DIRECT GRANT PROGRAMS How Grants Are Made Miscellaneous § 75.264 Transfers among budget categories. A grantee may, notwithstanding any...

  7. 40 CFR 264.343 - Performance standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Performance standards. 264.343 Section... Incinerators § 264.343 Performance standards. An incinerator burning hazardous waste must be designed... § 264.345, it will meet the following performance standards: (a)(1) Except as provided in paragraph (a...

  8. 40 CFR 264.343 - Performance standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Performance standards. 264.343 Section... Incinerators § 264.343 Performance standards. An incinerator burning hazardous waste must be designed... § 264.345, it will meet the following performance standards: (a)(1) Except as provided in paragraph (a...

  9. 40 CFR 264.343 - Performance standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Performance standards. 264.343 Section... Incinerators § 264.343 Performance standards. An incinerator burning hazardous waste must be designed... § 264.345, it will meet the following performance standards: (a)(1) Except as provided in paragraph (a...

  10. 40 CFR 264.343 - Performance standards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Performance standards. 264.343 Section... Incinerators § 264.343 Performance standards. An incinerator burning hazardous waste must be designed... § 264.345, it will meet the following performance standards: (a)(1) Except as provided in paragraph (a...

  11. 40 CFR 264.148 - Incapacity of owners or operators, guarantors, or financial institutions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., guarantors, or financial institutions. 264.148 Section 264.148 Protection of Environment ENVIRONMENTAL... TREATMENT, STORAGE, AND DISPOSAL FACILITIES Financial Requirements § 264.148 Incapacity of owners or operators, guarantors, or financial institutions. (a) An owner or operator must notify the Regional...

  12. 40 CFR 264.54 - Amendment of contingency plan.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Amendment of contingency plan. 264.54 Section 264.54 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Contingency Plan and Emergency Procedures § 264.54 Amendment of contingency plan. The contingency plan must be...

  13. 40 CFR 264.16 - Personnel training.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Personnel training. 264.16 Section 264... Facility Standards § 264.16 Personnel training. (a)(1) Facility personnel must successfully complete a program of classroom instruction or on-the-job training that teaches them to perform their duties in a way...

  14. 40 CFR 264.16 - Personnel training.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Personnel training. 264.16 Section 264... Facility Standards § 264.16 Personnel training. (a)(1) Facility personnel must successfully complete a program of classroom instruction or on-the-job training that teaches them to perform their duties in a way...

  15. 40 CFR 264.116 - Survey plat.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Survey plat. 264.116 Section 264.116...-Closure § 264.116 Survey plat. No later than the submission of the certification of closure of each... authority with jurisdiction over local land use, and to the Regional Administrator, a survey plat indicating...

  16. 40 CFR 264.116 - Survey plat.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Survey plat. 264.116 Section 264.116...-Closure § 264.116 Survey plat. No later than the submission of the certification of closure of each... authority with jurisdiction over local land use, and to the Regional Administrator, a survey plat indicating...

  17. 40 CFR 264.116 - Survey plat.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Survey plat. 264.116 Section 264.116...-Closure § 264.116 Survey plat. No later than the submission of the certification of closure of each... authority with jurisdiction over local land use, and to the Regional Administrator, a survey plat indicating...

  18. 40 CFR 264.116 - Survey plat.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Survey plat. 264.116 Section 264.116...-Closure § 264.116 Survey plat. No later than the submission of the certification of closure of each... authority with jurisdiction over local land use, and to the Regional Administrator, a survey plat indicating...

  19. 40 CFR 264.116 - Survey plat.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Survey plat. 264.116 Section 264.116...-Closure § 264.116 Survey plat. No later than the submission of the certification of closure of each... authority with jurisdiction over local land use, and to the Regional Administrator, a survey plat indicating...

  20. 40 CFR 264.119 - Post-closure notices.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 264.119 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... closure of each hazardous waste disposal unit, the owner or operator must submit to the local zoning... required by §§ 264.116 and 264.119(a) have been filed with the local zoning authority or the authority with...

  1. 40 CFR 264.119 - Post-closure notices.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 264.119 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... closure of each hazardous waste disposal unit, the owner or operator must submit to the local zoning... required by §§ 264.116 and 264.119(a) have been filed with the local zoning authority or the authority with...

  2. 40 CFR 264.151 - Wording of the instruments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Wording of the instruments. 264.151 Section 264.151 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Financial Requirements § 264.151 Wording o...

  3. 50 CFR 665.264 - Gear restrictions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Gear restrictions. 665.264 Section 665.264..., DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES IN THE WESTERN PACIFIC Hawaii Fisheries § 665.264 Gear restrictions. Only selective gear may be used to harvest coral from any precious coral permit area. ...

  4. 40 CFR 264.73 - Operating record.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... System, Recordkeeping, and Reporting § 264.73 Operating record. (a) The owner or operator must keep a written operating record at his facility. (b) The following information must be recorded, as it becomes... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Operating record. 264.73 Section 264...

  5. 39 CFR 264.2 - Policy.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 39 Postal Service 1 2011-07-01 2011-07-01 false Policy. 264.2 Section 264.2 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.2 Policy. It is the policy of the U.S. Postal Service to ensure the availability of all records considered critical to the...

  6. 39 CFR 264.2 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 39 Postal Service 1 2014-07-01 2014-07-01 false Policy. 264.2 Section 264.2 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION VITAL RECORDS § 264.2 Policy. It is the policy of the U.S. Postal Service to ensure the availability of all records considered critical to the...

  7. 40 CFR 264.280 - Closure and post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Closure and post-closure care. 264.280 Section 264.280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... § 264.278, except that soil-pore liquid monitoring may be terminated 90 days after the last application...

  8. 27 CFR 28.264 - Lading for exportation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Lading for exportation. 28.264 Section 28.264 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS EXPORTATION OF ALCOHOL Proceedings at Ports of Export § 28.264 Lading for...

  9. 27 CFR 28.264 - Lading for exportation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Lading for exportation. 28.264 Section 28.264 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS EXPORTATION OF ALCOHOL Proceedings at Ports of Export § 28.264 Lading for...

  10. 27 CFR 28.264 - Lading for exportation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Lading for exportation. 28.264 Section 28.264 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL EXPORTATION OF ALCOHOL Proceedings at Ports of Export § 28.264 Lading for...

  11. 27 CFR 28.264 - Lading for exportation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Lading for exportation. 28.264 Section 28.264 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL EXPORTATION OF ALCOHOL Proceedings at Ports of Export § 28.264 Lading for...

  12. 27 CFR 28.264 - Lading for exportation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Lading for exportation. 28.264 Section 28.264 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS EXPORTATION OF ALCOHOL Proceedings at Ports of Export § 28.264 Lading for...

  13. 40 CFR 721.10416 - Brominated polyphenyl ether (generic) (P-11-264).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) (P-11-264). 721.10416 Section 721.10416 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Specific Chemical Substances § 721.10416 Brominated polyphenyl ether (generic) (P-11-264). (a) Chemical... as brominated polyphenyl ether (PMN P-11-264) is subject to reporting under this section for the...

  14. 40 CFR 721.10416 - Brominated polyphenyl ether (generic) (P-11-264).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) (P-11-264). 721.10416 Section 721.10416 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Specific Chemical Substances § 721.10416 Brominated polyphenyl ether (generic) (P-11-264). (a) Chemical... as brominated polyphenyl ether (PMN P-11-264) is subject to reporting under this section for the...

  15. 40 CFR 721.10416 - Brominated polyphenyl ether (generic) (P-11-264).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) (P-11-264). 721.10416 Section 721.10416 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Specific Chemical Substances § 721.10416 Brominated polyphenyl ether (generic) (P-11-264). (a) Chemical... as brominated polyphenyl ether (PMN P-11-264) is subject to reporting under this section for the...

  16. 21 CFR 26.4 - Product coverage.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Product coverage. 26.4 Section 26.4 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL MUTUAL RECOGNITION OF... Provisions for Pharmaceutical Good Manufacturing Practices § 26.4 Product coverage. (a) The provisions of...

  17. 45 CFR 264.83 - How will we know if a Territory failed to meet the Matching Grant funding requirements at § 264.80?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the Matching Grant funding requirements at § 264.80? We will require the Territories to report the expenditures required by § 264.80(a)(2) and (a)(3) on the quarterly Territorial Financial Report. ...

  18. 40 CFR 264.222 - Action leakage rate.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Action leakage rate. 264.222 Section... Impoundments § 264.222 Action leakage rate. (a) The Regional Administrator shall approve an action leakage rate for surface impoundment units subject to § 264.221 (c) or (d). The action leakage rate is the maximum...

  19. 40 CFR 264.252 - Action leakage rate.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Action leakage rate. 264.252 Section... Piles § 264.252 Action leakage rate. (a) The Regional Administrator shall approve an action leakage rate for waste pile units subject to § 264.251(c) or (d). The action leakage rate is the maximum design...

  20. 40 CFR 264.302 - Action leakage rate.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Action leakage rate. 264.302 Section... § 264.302 Action leakage rate. (a) The Regional Administrator shall approve an action leakage rate for landfill units subject to § 264.301(c) or (d). The action leakage rate is the maximum design flow rate that...

  1. 40 CFR 264.92 - Ground-water protection standard.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Ground-water protection standard. 264... Releases From Solid Waste Management Units § 264.92 Ground-water protection standard. The owner or operator... constituents under § 264.93 detected in the ground water from a regulated unit do not exceed the concentration...

  2. 40 CFR 264.92 - Ground-water protection standard.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Ground-water protection standard. 264... Releases From Solid Waste Management Units § 264.92 Ground-water protection standard. The owner or operator... constituents under § 264.93 detected in the ground water from a regulated unit do not exceed the concentration...

  3. 40 CFR 264.276 - Food-chain crops.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Food-chain crops. 264.276 Section 264... Treatment § 264.276 Food-chain crops. The Regional Administrator may allow the growth of food-chain crops in... Regional Administrator will specify in the facility permit the specific food-chain crops which may be grown...

  4. 40 CFR 264.276 - Food-chain crops.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Food-chain crops. 264.276 Section 264... Treatment § 264.276 Food-chain crops. The Regional Administrator may allow the growth of food-chain crops in... Regional Administrator will specify in the facility permit the specific food-chain crops which may be grown...

  5. 40 CFR 264.276 - Food-chain crops.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Food-chain crops. 264.276 Section 264... Treatment § 264.276 Food-chain crops. The Regional Administrator may allow the growth of food-chain crops in... Regional Administrator will specify in the facility permit the specific food-chain crops which may be grown...

  6. 40 CFR 264.276 - Food-chain crops.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Food-chain crops. 264.276 Section 264... Treatment § 264.276 Food-chain crops. The Regional Administrator may allow the growth of food-chain crops in... Regional Administrator will specify in the facility permit the specific food-chain crops which may be grown...

  7. 40 CFR 264.276 - Food-chain crops.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Food-chain crops. 264.276 Section 264... Treatment § 264.276 Food-chain crops. The Regional Administrator may allow the growth of food-chain crops in... Regional Administrator will specify in the facility permit the specific food-chain crops which may be grown...

  8. 12 CFR 264a.3 - What special post-employment restrictions apply to senior examiners?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... to senior examiners? 264a.3 Section 264a.3 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM POST-EMPLOYMENT RESTRICTIONS FOR SENIOR EXAMINERS § 264a.3 What special post-employment restrictions apply to senior examiners? (a) Senior Examiners of State...

  9. 25 CFR 26.4 - Who administers the Job Placement and Training Program?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false Who administers the Job Placement and Training Program? 26.4 Section 26.4 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR HUMAN SERVICES JOB PLACEMENT AND TRAINING PROGRAM General Applicability § 26.4 Who administers the Job Placement and Training...

  10. 25 CFR 26.4 - Who administers the Job Placement and Training Program?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false Who administers the Job Placement and Training Program? 26.4 Section 26.4 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR HUMAN SERVICES JOB PLACEMENT AND TRAINING PROGRAM General Applicability § 26.4 Who administers the Job Placement and Training...

  11. 25 CFR 26.4 - Who administers the Job Placement and Training Program?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 1 2012-04-01 2011-04-01 true Who administers the Job Placement and Training Program? 26.4 Section 26.4 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR HUMAN SERVICES JOB PLACEMENT AND TRAINING PROGRAM General Applicability § 26.4 Who administers the Job Placement and Training...

  12. 25 CFR 26.4 - Who administers the Job Placement and Training Program?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false Who administers the Job Placement and Training Program? 26.4 Section 26.4 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR HUMAN SERVICES JOB PLACEMENT AND TRAINING PROGRAM General Applicability § 26.4 Who administers the Job Placement and Training...

  13. 40 CFR 264.3 - Relationship to interim status standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Relationship to interim status standards. 264.3 Section 264.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES General § 264.3 Relationship to...

  14. 40 CFR 264.92 - Ground-water protection standard.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Ground-water protection standard. 264... Releases From Solid Waste Management Units § 264.92 Ground-water protection standard. The owner or operator... constituents under § 264.93 detected in the ground water from a regulated unit do not exceed the concentration...

  15. 40 CFR 264.92 - Ground-water protection standard.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Ground-water protection standard. 264... Releases From Solid Waste Management Units § 264.92 Ground-water protection standard. The owner or operator... constituents under § 264.93 detected in the ground water from a regulated unit do not exceed the concentration...

  16. 40 CFR 264.92 - Ground-water protection standard.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Ground-water protection standard. 264... Releases From Solid Waste Management Units § 264.92 Ground-water protection standard. The owner or operator... constituents under § 264.93 detected in the ground water from a regulated unit do not exceed the concentration...

  17. 27 CFR 20.264 - User's records and report of products and processes.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of products and processes. 20.264 Section 20.264 Alcohol, Tobacco Products and Firearms ALCOHOL AND... AND RUM Records and Reports § 20.264 User's records and report of products and processes. (a) Records. (1) Each user shall maintain separate accountings of— (i) The number of gallons of each formula of...

  18. 40 CFR 264.316 - Disposal of small containers of hazardous waste in overpacked drums (lab packs).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Landfills § 264.316 Disposal of small containers of hazardous waste in overpacked drums (lab packs). Small containers of hazardous waste in overpacked... hazardous waste in overpacked drums (lab packs). 264.316 Section 264.316 Protection of Environment...

  19. 27 CFR 26.264 - Determination of tax on wine.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... wine. 26.264 Section 26.264 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Procedure at Port of Entry From the Virgin Islands § 26.264 Determination of tax on wine. If the certificate prescribed in § 26.205 covers wine, the wine tax will be collected at the rates imposed by section 5041...

  20. 40 CFR 264.341 - Waste analysis.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Waste analysis. 264.341 Section 264... Incinerators § 264.341 Waste analysis. (a) As a portion of the trial burn plan required by § 270.62 of this chapter, or with part B of the permit application, the owner or operator must have included an analysis of...

  1. 40 CFR 264.572 - Design and installation of new drip pads.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Design and installation of new drip pads. 264.572 Section 264.572 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Drip Pads § 264.572 Design...

  2. 40 CFR 264.341 - Waste analysis.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Waste analysis. 264.341 Section 264... Incinerators § 264.341 Waste analysis. (a) As a portion of the trial burn plan required by § 270.62 of this chapter, or with part B of the permit application, the owner or operator must have included an analysis of...

  3. 40 CFR 264.341 - Waste analysis.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Waste analysis. 264.341 Section 264... Incinerators § 264.341 Waste analysis. (a) As a portion of the trial burn plan required by § 270.62 of this chapter, or with part B of the permit application, the owner or operator must have included an analysis of...

  4. 45 CFR 264.77 - How will we determine if a State met its Contingency Fund expenditure requirements?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Contingency Fund expenditure requirements? 264.77 Section 264.77 Public Welfare Regulations Relating to Public... Contingency Fund? § 264.77 How will we determine if a State met its Contingency Fund expenditure requirements? (a) States receiving contingency funds for a fiscal year must complete the quarterly TANF Financial...

  5. 45 CFR 264.80 - If a Territory receives Matching Grant funds, what funds must it expend?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... funds must it expend? 264.80 Section 264.80 Public Welfare Regulations Relating to Public Welfare OFFICE... Levels of the Territories? § 264.80 If a Territory receives Matching Grant funds, what funds must it expend? (a) If a Territory receives Matching Grant funds under section 1108(b) of the Act, it must: (1...

  6. 27 CFR 44.264a - To a foreign-trade zone.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2012-04-01 2011-04-01 true To a foreign-trade zone. 44.264a Section 44.264a Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU... Removal of Shipment § 44.264a To a foreign-trade zone. Where cigars are withdrawn from a customs warehouse...

  7. 27 CFR 44.264a - To a foreign-trade zone.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2014-04-01 2014-04-01 false To a foreign-trade zone. 44.264a Section 44.264a Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Warehouses Notice of Removal of Shipment § 44.264a To a foreign-trade zone. Where cigars are withdrawn from a...

  8. 27 CFR 44.264a - To a foreign-trade zone.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2013-04-01 2013-04-01 false To a foreign-trade zone. 44.264a Section 44.264a Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Warehouses Notice of Removal of Shipment § 44.264a To a foreign-trade zone. Where cigars are withdrawn from a...

  9. 27 CFR 44.264a - To a foreign-trade zone.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false To a foreign-trade zone. 44.264a Section 44.264a Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Warehouses Notice of Removal of Shipment § 44.264a To a foreign-trade zone. Where cigars are withdrawn from a...

  10. 40 CFR 264.231 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.231 Section 264.231 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Surface Impoundments § 264.231 Special...

  11. 40 CFR 264.317 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.317 Section 264.317 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Landfills § 264.317 Special requirements for...

  12. 40 CFR 264.317 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.317 Section 264.317 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Landfills § 264.317 Special requirements for...

  13. 40 CFR 264.231 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.231 Section 264.231 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Surface Impoundments § 264.231 Special...

  14. 40 CFR 264.231 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.231 Section 264.231 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Surface Impoundments § 264.231 Special...

  15. 40 CFR 264.317 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.317 Section 264.317 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Landfills § 264.317 Special requirements for...

  16. H.264/AVC Video Compression on Smartphones

    NASA Astrophysics Data System (ADS)

    Sharabayko, M. P.; Markov, N. G.

    2017-01-01

    In this paper, we studied the usage of H.264/AVC video compression tools by the flagship smartphones. The results show that only a subset of tools is used, meaning that there is still a potential to achieve higher compression efficiency within the H.264/AVC standard, but the most advanced smartphones are already reaching the compression efficiency limit of H.264/AVC.

  17. 20 CFR 1002.264 - Is the employee allowed to repay a previous distribution from a pension benefits plan upon being...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... distribution from a pension benefits plan upon being reemployed? 1002.264 Section 1002.264 Employees' Benefits... and Benefits Pension Plan Benefits § 1002.264 Is the employee allowed to repay a previous distribution from a pension benefits plan upon being reemployed? Yes, provided the plan is a defined benefit plan...

  18. 40 CFR 264.283 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.283 Section 264.283 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.283 Special requirements...

  19. 40 CFR 264.283 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.283 Section 264.283 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.283 Special requirements...

  20. 40 CFR 264.283 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.283 Section 264.283 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.283 Special requirements...

  1. 40 CFR 264.283 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.283 Section 264.283 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.283 Special requirements...

  2. 45 CFR 264.72 - What requirements are imposed on a State if it receives contingency funds?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... receives contingency funds? 264.72 Section 264.72 Public Welfare Regulations Relating to Public Welfare... Contingency Fund? § 264.72 What requirements are imposed on a State if it receives contingency funds? (a)(1) A State must meet a Contingency Fund MOE level of 100 percent of historic State expenditures for FY 1994...

  3. 45 CFR 264.70 - What makes a State eligible to receive a provisional payment of contingency funds?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... provisional payment of contingency funds? 264.70 Section 264.70 Public Welfare Regulations Relating to Public... Contingency Fund? § 264.70 What makes a State eligible to receive a provisional payment of contingency funds? (a) In order to receive a provisional payment of contingency funds, a State must: (1) Be a needy...

  4. 40 CFR 264.259 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.259 Section 264.259 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Waste Piles § 264.259 Special requirements for...

  5. 40 CFR 264.259 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.259 Section 264.259 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Waste Piles § 264.259 Special requirements for...

  6. 40 CFR 264.259 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.259 Section 264.259 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Waste Piles § 264.259 Special requirements for...

  7. 26 CFR 1.264-1 - Premiums on life insurance taken out in a trade or business.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 3 2012-04-01 2012-04-01 false Premiums on life insurance taken out in a trade or business. 1.264-1 Section 1.264-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.264-1 Premiums on life insurance taken out in a trade or...

  8. 45 CFR 264.71 - What determines the amount of the provisional payment of contingency funds that will be made to a...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... payment of contingency funds that will be made to a State? 264.71 Section 264.71 Public Welfare... Requirements for the Contingency Fund? § 264.71 What determines the amount of the provisional payment of contingency funds that will be made to a State? We will make a provisional payment to a State that meets the...

  9. 36 CFR 264.11 - Use of symbol.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MANAGEMENT Mount St. Helens National Volcanic Monument Symbol § 264.11 Use of symbol. Except as provided in § 264.12, use of the Mount St. Helens National Volcanic Monument official symbol, including a facsimile...

  10. 45 CFR 264.76 - What action will we take if a State fails to remit funds after failing to meet its required...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false What action will we take if a State fails to remit funds after failing to meet its required Contingency Fund MOE level? 264.76 Section 264.76 Public... What Are the Requirements for the Contingency Fund? § 264.76 What action will we take if a State fails...

  11. 45 CFR 264.76 - What action will we take if a State fails to remit funds after failing to meet its required...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false What action will we take if a State fails to remit funds after failing to meet its required Contingency Fund MOE level? 264.76 Section 264.76 Public... What Are the Requirements for the Contingency Fund? § 264.76 What action will we take if a State fails...

  12. 45 CFR 264.76 - What action will we take if a State fails to remit funds after failing to meet its required...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true What action will we take if a State fails to remit funds after failing to meet its required Contingency Fund MOE level? 264.76 Section 264.76 Public... What Are the Requirements for the Contingency Fund? § 264.76 What action will we take if a State fails...

  13. 45 CFR 264.76 - What action will we take if a State fails to remit funds after failing to meet its required...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true What action will we take if a State fails to remit funds after failing to meet its required Contingency Fund MOE level? 264.76 Section 264.76 Public... What Are the Requirements for the Contingency Fund? § 264.76 What action will we take if a State fails...

  14. 45 CFR 264.76 - What action will we take if a State fails to remit funds after failing to meet its required...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false What action will we take if a State fails to remit funds after failing to meet its required Contingency Fund MOE level? 264.76 Section 264.76 Public... What Are the Requirements for the Contingency Fund? § 264.76 What action will we take if a State fails...

  15. Mode-dependent templates and scan order for H.264/AVC-based intra lossless coding.

    PubMed

    Gu, Zhouye; Lin, Weisi; Lee, Bu-Sung; Lau, Chiew Tong; Sun, Ming-Ting

    2012-09-01

    In H.264/advanced video coding (AVC), lossless coding and lossy coding share the same entropy coding module. However, the entropy coders in the H.264/AVC standard were original designed for lossy video coding and do not yield adequate performance for lossless video coding. In this paper, we analyze the problem with the current lossless coding scheme and propose a mode-dependent template (MD-template) based method for intra lossless coding. By exploring the statistical redundancy of the prediction residual in the H.264/AVC intra prediction modes, more zero coefficients are generated. By designing a new scan order for each MD-template, the scanned coefficients sequence fits the H.264/AVC entropy coders better. A fast implementation algorithm is also designed. With little computation increase, experimental results confirm that the proposed fast algorithm achieves about 7.2% bit saving compared with the current H.264/AVC fidelity range extensions high profile.

  16. Transcoding method from H.264/AVC to high efficiency video coding based on similarity of intraprediction, interprediction, and motion vector

    NASA Astrophysics Data System (ADS)

    Liu, Mei-Feng; Zhong, Guo-Yun; He, Xiao-Hai; Qing, Lin-Bo

    2016-09-01

    Currently, most video resources on line are encoded in the H.264/AVC format. More fluent video transmission can be obtained if these resources are encoded in the newest international video coding standard: high efficiency video coding (HEVC). In order to improve the video transmission and storage on line, a transcoding method from H.264/AVC to HEVC is proposed. In this transcoding algorithm, the coding information of intraprediction, interprediction, and motion vector (MV) in H.264/AVC video stream are used to accelerate the coding in HEVC. It is found through experiments that the region of interprediction in HEVC overlaps that in H.264/AVC. Therefore, the intraprediction for the region in HEVC, which is interpredicted in H.264/AVC, can be skipped to reduce coding complexity. Several macroblocks in H.264/AVC are combined into one PU in HEVC when the MV difference between two of the macroblocks in H.264/AVC is lower than a threshold. This method selects only one coding unit depth and one prediction unit (PU) mode to reduce the coding complexity. An MV interpolation method of combined PU in HEVC is proposed according to the areas and distances between the center of one macroblock in H.264/AVC and that of the PU in HEVC. The predicted MV accelerates the motion estimation for HEVC coding. The simulation results show that our proposed algorithm achieves significant coding time reduction with a little loss in bitrates distortion rate, compared to the existing transcoding algorithms and normal HEVC coding.

  17. 40 CFR 264.571 - Assessment of existing drip pad integrity.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... leak detection systems of § 264.573(b). No later than the effective date of this rule, the owner or..., except the standards for liners and leak detection systems, specified in § 264.573(b). (b) The owner or...

  18. 40 CFR 264.95 - Point of compliance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 264.95 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Releases From Solid Waste Management Units § 264.95 Point of compliance. (a) The Regional Administrator will...

  19. 40 CFR 264.95 - Point of compliance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 264.95 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Releases From Solid Waste Management Units § 264.95 Point of compliance. (a) The Regional Administrator will...

  20. 40 CFR 264.178 - Closure.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Closure. 264.178 Section 264.178 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR... from the containment system. Remaining containers, liners, bases, and soil containing or contaminated...

  1. 40 CFR 264.194 - General operating requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....194 Section 264.194 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Tank Systems § 264.194 General operating requirements. (a) Hazardous wastes or treatment reagents must...

  2. 40 CFR 264.270 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264... treat or dispose of hazardous waste in land treatment units, except as § 264.1 provides otherwise. ...

  3. 40 CFR 264.270 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264... treat or dispose of hazardous waste in land treatment units, except as § 264.1 provides otherwise. ...

  4. 40 CFR 264.270 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264... treat or dispose of hazardous waste in land treatment units, except as § 264.1 provides otherwise. ...

  5. 40 CFR 264.272 - Treatment demonstration.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 264.272 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.272 Treatment demonstration. (a) For each waste that will be applied to the...

  6. 40 CFR 264.272 - Treatment demonstration.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 264.272 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.272 Treatment demonstration. (a) For each waste that will be applied to the...

  7. 40 CFR 264.270 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264... treat or dispose of hazardous waste in land treatment units, except as § 264.1 provides otherwise. ...

  8. 40 CFR 264.270 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264... treat or dispose of hazardous waste in land treatment units, except as § 264.1 provides otherwise. ...

  9. 40 CFR 264.272 - Treatment demonstration.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 264.272 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.272 Treatment demonstration. (a) For each waste that will be applied to the...

  10. 40 CFR 264.272 - Treatment demonstration.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 264.272 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.272 Treatment demonstration. (a) For each waste that will be applied to the...

  11. 40 CFR 264.272 - Treatment demonstration.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 264.272 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.272 Treatment demonstration. (a) For each waste that will be applied to the...

  12. 40 CFR 264.50 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Applicability. 264.50 Section 264.50 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Contingency Plan and...

  13. Adapting hierarchical bidirectional inter prediction on a GPU-based platform for 2D and 3D H.264 video coding

    NASA Astrophysics Data System (ADS)

    Rodríguez-Sánchez, Rafael; Martínez, José Luis; Cock, Jan De; Fernández-Escribano, Gerardo; Pieters, Bart; Sánchez, José L.; Claver, José M.; de Walle, Rik Van

    2013-12-01

    The H.264/AVC video coding standard introduces some improved tools in order to increase compression efficiency. Moreover, the multi-view extension of H.264/AVC, called H.264/MVC, adopts many of them. Among the new features, variable block-size motion estimation is one which contributes to high coding efficiency. Furthermore, it defines a different prediction structure that includes hierarchical bidirectional pictures, outperforming traditional Group of Pictures patterns in both scenarios: single-view and multi-view. However, these video coding techniques have high computational complexity. Several techniques have been proposed in the literature over the last few years which are aimed at accelerating the inter prediction process, but there are no works focusing on bidirectional prediction or hierarchical prediction. In this article, with the emergence of many-core processors or accelerators, a step forward is taken towards an implementation of an H.264/AVC and H.264/MVC inter prediction algorithm on a graphics processing unit. The results show a negligible rate distortion drop with a time reduction of up to 98% for the complete H.264/AVC encoder.

  14. 40 CFR 264.601 - Environmental performance standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Environmental performance standards. 264.601 Section 264.601 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... of human health and the environment. Permits for miscellaneous units are to contain such terms and...

  15. 40 CFR 264.340 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Incinerators § 264... waste incinerators (as defined in § 260.10 of this chapter), except as § 264.1 provides otherwise. (b...

  16. 40 CFR 264.4 - Imminent hazard action.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Imminent hazard action. 264.4 Section 264.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES General...

  17. 40 CFR 264.150 - State assumption of responsibility.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false State assumption of responsibility. 264.150 Section 264.150 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL...

  18. 40 CFR 264.13 - General waste analysis.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false General waste analysis. 264.13 Section 264.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES General...

  19. 40 CFR 264.76 - Unmanifested waste report.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Unmanifested waste report. 264.76 Section 264.76 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  20. 40 CFR 264.13 - General waste analysis.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false General waste analysis. 264.13 Section 264.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES General...

  1. 40 CFR 264.13 - General waste analysis.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false General waste analysis. 264.13 Section 264.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES General...

  2. 40 CFR 264.13 - General waste analysis.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false General waste analysis. 264.13 Section 264.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES General...

  3. 40 CFR 264.76 - Unmanifested waste report.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Unmanifested waste report. 264.76 Section 264.76 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  4. 40 CFR 264.76 - Unmanifested waste report.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Unmanifested waste report. 264.76 Section 264.76 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  5. 40 CFR 264.76 - Unmanifested waste report.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Unmanifested waste report. 264.76 Section 264.76 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  6. 40 CFR 264.76 - Unmanifested waste report.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Unmanifested waste report. 264.76 Section 264.76 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  7. 40 CFR 264.1200 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Waste Munitions and Explosives Storage § 264.1200 Applicability. The requirements of this subpart apply to owners or operators who store munitions and explosive hazardous wastes, except as § 264.1 provides otherwise. (NOTE: Depending on explosive hazards, hazardous waste munitions and explosives may also be...

  8. 40 CFR 264.1200 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Waste Munitions and Explosives Storage § 264.1200 Applicability. The requirements of this subpart apply to owners or operators who store munitions and explosive hazardous wastes, except as § 264.1 provides otherwise. (NOTE: Depending on explosive hazards, hazardous waste munitions and explosives may also be...

  9. 40 CFR 264.1200 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Waste Munitions and Explosives Storage § 264.1200 Applicability. The requirements of this subpart apply to owners or operators who store munitions and explosive hazardous wastes, except as § 264.1 provides otherwise. (NOTE: Depending on explosive hazards, hazardous waste munitions and explosives may also be...

  10. 40 CFR 264.1200 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Waste Munitions and Explosives Storage § 264.1200 Applicability. The requirements of this subpart apply to owners or operators who store munitions and explosive hazardous wastes, except as § 264.1 provides otherwise. (NOTE: Depending on explosive hazards, hazardous waste munitions and explosives may also be...

  11. 40 CFR 264.1200 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Waste Munitions and Explosives Storage § 264.1200 Applicability. The requirements of this subpart apply to owners or operators who store munitions and explosive hazardous wastes, except as § 264.1 provides otherwise. (NOTE: Depending on explosive hazards, hazardous waste munitions and explosives may also be...

  12. 40 CFR 264.273 - Design and operating requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....273 Section 264.273 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.273 Design and operating requirements. The Regional Administrator will specify in...

  13. 40 CFR 264.273 - Design and operating requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....273 Section 264.273 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.273 Design and operating requirements. The Regional Administrator will specify in...

  14. 40 CFR 264.273 - Design and operating requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....273 Section 264.273 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.273 Design and operating requirements. The Regional Administrator will specify in...

  15. 40 CFR 264.273 - Design and operating requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....273 Section 264.273 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.273 Design and operating requirements. The Regional Administrator will specify in...

  16. 40 CFR 264.273 - Design and operating requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....273 Section 264.273 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.273 Design and operating requirements. The Regional Administrator will specify in...

  17. 40 CFR 264.1083 - Waste determination procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Waste determination procedures. 264... WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1083 Waste...

  18. 40 CFR 264.198 - Special requirements for ignitable or reactive wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... waste, mixture, or dissolved material no longer meets the definition of ignitable or reactive waste... comply with the requirements for the maintenance of protective distances between the waste management... reactive wastes. 264.198 Section 264.198 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...

  19. 40 CFR 264.142 - Cost estimate for closure.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 264.142 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... may be realized with the sale of hazardous wastes, or non-hazardous wastes if applicable under § 264...

  20. 40 CFR 264.142 - Cost estimate for closure.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 264.142 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... may be realized with the sale of hazardous wastes, or non-hazardous wastes if applicable under § 264...

  1. 40 CFR 264.142 - Cost estimate for closure.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 264.142 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... may be realized with the sale of hazardous wastes, or non-hazardous wastes if applicable under § 264...

  2. 40 CFR 264.142 - Cost estimate for closure.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 264.142 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... may be realized with the sale of hazardous wastes, or non-hazardous wastes if applicable under § 264...

  3. 40 CFR 264.1201 - Design and operating standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Design and operating standards. 264... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1201 Design and operating standards. (a... Operating Procedure specifying procedures to ensure safety, security, and environmental protection. If these...

  4. 40 CFR 264.1201 - Design and operating standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Design and operating standards. 264... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1201 Design and operating standards. (a... Operating Procedure specifying procedures to ensure safety, security, and environmental protection. If these...

  5. 40 CFR 264.1201 - Design and operating standards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Design and operating standards. 264... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1201 Design and operating standards. (a... Operating Procedure specifying procedures to ensure safety, security, and environmental protection. If these...

  6. 40 CFR 264.1201 - Design and operating standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Design and operating standards. 264... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1201 Design and operating standards. (a... Operating Procedure specifying procedures to ensure safety, security, and environmental protection. If these...

  7. 40 CFR 264.603 - Post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Post-closure care. 264.603 Section 264.603 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... treatment or storage unit has contaminated soils or ground water that cannot be completely removed or...

  8. 40 CFR 264.1086 - Standards: Containers.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... integral part of the container structural design (e.g., a “portable tank” or bulk cargo container equipped... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Standards: Containers. 264.1086... Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1086 Standards...

  9. 40 CFR 264.1086 - Standards: Containers.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... integral part of the container structural design (e.g., a “portable tank” or bulk cargo container equipped... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Standards: Containers. 264.1086... Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1086 Standards...

  10. 40 CFR 264.1086 - Standards: Containers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... integral part of the container structural design (e.g., a “portable tank” or bulk cargo container equipped... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Standards: Containers. 264.1086... Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1086 Standards...

  11. 40 CFR 264.1086 - Standards: Containers.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... integral part of the container structural design (e.g., a “portable tank” or bulk cargo container equipped... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Standards: Containers. 264.1086... Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1086 Standards...

  12. 40 CFR 264.1201 - Design and operating standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Design and operating standards. 264.1201 Section 264.1201 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... inspection procedures that assure the controls and containment systems are working as designed and that...

  13. 38 CFR 26.4 - Policy.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Policy. 26.4 Section 26.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) ENVIRONMENTAL EFFECTS OF... toward the maximum attainable recycling of nonrenewable resources. (b) VA elements shall: (1) Interpret...

  14. 38 CFR 26.4 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Policy. 26.4 Section 26.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) ENVIRONMENTAL EFFECTS OF... toward the maximum attainable recycling of nonrenewable resources. (b) VA elements shall: (1) Interpret...

  15. 38 CFR 26.4 - Policy.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Policy. 26.4 Section 26.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) ENVIRONMENTAL EFFECTS OF... toward the maximum attainable recycling of nonrenewable resources. (b) VA elements shall: (1) Interpret...

  16. 38 CFR 26.4 - Policy.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Policy. 26.4 Section 26.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) ENVIRONMENTAL EFFECTS OF... toward the maximum attainable recycling of nonrenewable resources. (b) VA elements shall: (1) Interpret...

  17. 38 CFR 26.4 - Policy.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Policy. 26.4 Section 26.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) ENVIRONMENTAL EFFECTS OF... toward the maximum attainable recycling of nonrenewable resources. (b) VA elements shall: (1) Interpret...

  18. 40 CFR 264.554 - Staging piles.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Staging piles. 264.554 Section 264.554 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR... environment; and (3) Any additional information the Director determines is necessary to protect human health...

  19. 40 CFR 264.554 - Staging piles.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Staging piles. 264.554 Section 264.554 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR... environment; and (3) Any additional information the Director determines is necessary to protect human health...

  20. 40 CFR 264.554 - Staging piles.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Staging piles. 264.554 Section 264.554 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR... environment; and (3) Any additional information the Director determines is necessary to protect human health...

  1. 40 CFR 264.554 - Staging piles.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Staging piles. 264.554 Section 264.554 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR... environment; and (3) Any additional information the Director determines is necessary to protect human health...

  2. 40 CFR 264.280 - Closure and post-closure care.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 264.280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.280 Closure and post-closure care. (a) During the closure period the owner or...

  3. 40 CFR 264.280 - Closure and post-closure care.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 264.280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.280 Closure and post-closure care. (a) During the closure period the owner or...

  4. 40 CFR 264.280 - Closure and post-closure care.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 264.280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.280 Closure and post-closure care. (a) During the closure period the owner or...

  5. 40 CFR 264.280 - Closure and post-closure care.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 264.280 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.280 Closure and post-closure care. (a) During the closure period the owner or...

  6. 40 CFR 264.114 - Disposal or decontamination of equipment, structures and soils.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... equipment, structures and soils. 264.114 Section 264.114 Protection of Environment ENVIRONMENTAL PROTECTION... of equipment, structures and soils. During the partial and final closure periods, all contaminated equipment, structures and soils must be properly disposed of or decontaminated unless otherwise specified in...

  7. 40 CFR 264.114 - Disposal or decontamination of equipment, structures and soils.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... equipment, structures and soils. 264.114 Section 264.114 Protection of Environment ENVIRONMENTAL PROTECTION... of equipment, structures and soils. During the partial and final closure periods, all contaminated equipment, structures and soils must be properly disposed of or decontaminated unless otherwise specified in...

  8. 40 CFR 264.114 - Disposal or decontamination of equipment, structures and soils.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... equipment, structures and soils. 264.114 Section 264.114 Protection of Environment ENVIRONMENTAL PROTECTION... of equipment, structures and soils. During the partial and final closure periods, all contaminated equipment, structures and soils must be properly disposed of or decontaminated unless otherwise specified in...

  9. 40 CFR 264.114 - Disposal or decontamination of equipment, structures and soils.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... equipment, structures and soils. 264.114 Section 264.114 Protection of Environment ENVIRONMENTAL PROTECTION... of equipment, structures and soils. During the partial and final closure periods, all contaminated equipment, structures and soils must be properly disposed of or decontaminated unless otherwise specified in...

  10. 40 CFR 264.114 - Disposal or decontamination of equipment, structures and soils.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... equipment, structures and soils. 264.114 Section 264.114 Protection of Environment ENVIRONMENTAL PROTECTION... of equipment, structures and soils. During the partial and final closure periods, all contaminated equipment, structures and soils must be properly disposed of or decontaminated unless otherwise specified in...

  11. 40 CFR 264.140 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... applying to a regulated unit with alternative requirements for financial assurance set out in the permit or... OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Financial...) Prescribes alternative requirements for the regulated unit under § 264.90(f) and/or § 264.110(c); and (2...

  12. 40 CFR 264.99 - Compliance monitoring program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... be based on a compliance monitoring program developed to meet the requirements of this section. (i... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Compliance monitoring program. 264.99... Releases From Solid Waste Management Units § 264.99 Compliance monitoring program. An owner or operator...

  13. 40 CFR 264.309 - Surveying and recordkeeping.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Surveying and recordkeeping. 264.309... Landfills § 264.309 Surveying and recordkeeping. The owner or operator of a landfill must maintain the... dimensions, including depth, of each cell with respect to permanently surveyed benchmarks; and (b) The...

  14. 40 CFR 264.309 - Surveying and recordkeeping.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Surveying and recordkeeping. 264.309... Landfills § 264.309 Surveying and recordkeeping. The owner or operator of a landfill must maintain the... dimensions, including depth, of each cell with respect to permanently surveyed benchmarks; and (b) The...

  15. 40 CFR 264.309 - Surveying and recordkeeping.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Surveying and recordkeeping. 264.309... Landfills § 264.309 Surveying and recordkeeping. The owner or operator of a landfill must maintain the... dimensions, including depth, of each cell with respect to permanently surveyed benchmarks; and (b) The...

  16. 40 CFR 264.309 - Surveying and recordkeeping.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Surveying and recordkeeping. 264.309... Landfills § 264.309 Surveying and recordkeeping. The owner or operator of a landfill must maintain the... dimensions, including depth, of each cell with respect to permanently surveyed benchmarks; and (b) The...

  17. 40 CFR 264.1034 - Test methods and procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Test methods and procedures. 264.1034... Air Emission Standards for Process Vents § 264.1034 Test methods and procedures. (a) Each owner or operator subject to the provisions of this subpart shall comply with the test methods and procedures...

  18. 40 CFR 264.1063 - Test methods and procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Test methods and procedures. 264.1063... Air Emission Standards for Equipment Leaks § 264.1063 Test methods and procedures. (a) Each owner or operator subject to the provisions of this subpart shall comply with the test methods and procedures...

  19. 40 CFR 264.555 - Disposal of CAMU-eligible wastes in permitted hazardous waste landfills.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... permitted hazardous waste landfills. 264.555 Section 264.555 Protection of Environment ENVIRONMENTAL...-eligible wastes in permitted hazardous waste landfills. (a) The Regional Administrator with regulatory... hazardous waste landfills not located at the site from which the waste originated, without the wastes...

  20. 45 CFR 264.75 - For the annual reconciliation, what are qualifying State expenditures?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...)(5) of this chapter. (b) Qualifying State expenditures do not include: (1) Child care expenditures... State expenditures? 264.75 Section 264.75 Public Welfare Regulations Relating to Public Welfare OFFICE... HEALTH AND HUMAN SERVICES OTHER ACCOUNTABILITY PROVISIONS What Are the Requirements for the Contingency...

  1. 45 CFR 264.75 - For the annual reconciliation, what are qualifying State expenditures?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...)(5) of this chapter. (b) Qualifying State expenditures do not include: (1) Child care expenditures... State expenditures? 264.75 Section 264.75 Public Welfare Regulations Relating to Public Welfare OFFICE... HEALTH AND HUMAN SERVICES OTHER ACCOUNTABILITY PROVISIONS What Are the Requirements for the Contingency...

  2. 45 CFR 264.75 - For the annual reconciliation, what are qualifying State expenditures?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...)(5) of this chapter. (b) Qualifying State expenditures do not include: (1) Child care expenditures... State expenditures? 264.75 Section 264.75 Public Welfare Regulations Relating to Public Welfare OFFICE... HEALTH AND HUMAN SERVICES OTHER ACCOUNTABILITY PROVISIONS What Are the Requirements for the Contingency...

  3. 45 CFR 264.75 - For the annual reconciliation, what are qualifying State expenditures?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...)(5) of this chapter. (b) Qualifying State expenditures do not include: (1) Child care expenditures... State expenditures? 264.75 Section 264.75 Public Welfare Regulations Relating to Public Welfare OFFICE... HEALTH AND HUMAN SERVICES OTHER ACCOUNTABILITY PROVISIONS What Are the Requirements for the Contingency...

  4. 45 CFR 264.75 - For the annual reconciliation, what are qualifying State expenditures?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...)(5) of this chapter. (b) Qualifying State expenditures do not include: (1) Child care expenditures... State expenditures? 264.75 Section 264.75 Public Welfare Regulations Relating to Public Welfare OFFICE... HEALTH AND HUMAN SERVICES OTHER ACCOUNTABILITY PROVISIONS What Are the Requirements for the Contingency...

  5. 20 CFR 416.264 - When does the special SSI eligibility status apply.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false When does the special SSI eligibility status apply. 416.264 Section 416.264 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A...

  6. 40 CFR 264.1054 - Standards: Pressure relief devices in gas/vapor service.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... gas/vapor service. 264.1054 Section 264.1054 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE... relief devices in gas/vapor service. (a) Except during pressure releases, each pressure relief device in...

  7. 40 CFR 264.1087 - Standards: Closed-vent systems and control devices.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Standards: Closed-vent systems and control devices. 264.1087 Section 264.1087 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY....1087 Standards: Closed-vent systems and control devices. (a) This section applies to each closed-vent...

  8. 40 CFR 264.173 - Management of containers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Management of containers. 264.173... Use and Management of Containers § 264.173 Management of containers. (a) A container holding hazardous... container holding hazardous waste must not be opened, handled, or stored in a manner which may rupture the...

  9. 40 CFR 264.171 - Condition of containers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Condition of containers. 264.171... Use and Management of Containers § 264.171 Condition of containers. If a container holding hazardous... leak, the owner or operator must transfer the hazardous waste from this container to a container that...

  10. 40 CFR 264.314 - Special requirements for bulk and containerized liquids.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... containerized liquids. 264.314 Section 264.314 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... test must be used: Method 9095B (Paint Filter Liquids Test) as described in “Test Methods for Evaluating Solid Waste, Physical/Chemical Methods,” EPA Publication SW-846, as incorporated by reference in...

  11. 40 CFR 264.281 - Special requirements for ignitable or reactive waste.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Special requirements for ignitable or reactive waste. 264.281 Section 264.281 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... immediately incorporated into the soil so that: (1) The resulting waste, mixture, or dissolution of material...

  12. 40 CFR 264.51 - Purpose and implementation of contingency plan.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Purpose and implementation of contingency plan. 264.51 Section 264.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... plan must be designed to minimize hazards to human health or the environment from fires, explosions, or...

  13. 40 CFR 264.31 - Design and operation of facility.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Design and operation of facility. 264.31 Section 264.31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES...-sudden release of hazardous waste or hazardous waste constituents to air, soil, or surface water which...

  14. 40 CFR 60.264 - Emission monitoring.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Emission monitoring. 60.264 Section 60... Facilities § 60.264 Emission monitoring. (a) The owner or operator subject to the provisions of this subpart shall install, calibrate, maintain and operate a continuous monitoring system for measurement of the...

  15. 40 CFR 264.97 - General ground-water monitoring requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false General ground-water monitoring requirements. 264.97 Section 264.97 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Releases From Solid Waste...

  16. 40 CFR 264.54 - Amendment of contingency plan.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Amendment of contingency plan. 264.54... Contingency Plan and Emergency Procedures § 264.54 Amendment of contingency plan. The contingency plan must be reviewed, and immediately amended, if necessary, whenever: (a) The facility permit is revised; (b) The plan...

  17. 40 CFR 264.54 - Amendment of contingency plan.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Amendment of contingency plan. 264.54... Contingency Plan and Emergency Procedures § 264.54 Amendment of contingency plan. The contingency plan must be reviewed, and immediately amended, if necessary, whenever: (a) The facility permit is revised; (b) The plan...

  18. 40 CFR 264.174 - Inspections.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Containers § 264.174 Inspections. At least weekly, the owner or operator must inspect areas where containers are stored, except for Performance Track member facilities, that may conduct inspections at least once... Track member facility must follow the procedures identified in § 264.15(b)(5) of this part. The owner or...

  19. 40 CFR 264.174 - Inspections.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Containers § 264.174 Inspections. At least weekly, the owner or operator must inspect areas where containers are stored, except for Performance Track member facilities, that may conduct inspections at least once... Track member facility must follow the procedures identified in § 264.15(b)(5) of this part. The owner or...

  20. 40 CFR 264.174 - Inspections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Containers § 264.174 Inspections. At least weekly, the owner or operator must inspect areas where containers are stored, except for Performance Track member facilities, that may conduct inspections at least once... Track member facility must follow the procedures identified in § 264.15(b)(5) of this part. The owner or...

  1. 40 CFR 264.174 - Inspections.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Containers § 264.174 Inspections. At least weekly, the owner or operator must inspect areas where containers are stored, except for Performance Track member facilities, that may conduct inspections at least once... Track member facility must follow the procedures identified in § 264.15(b)(5) of this part. The owner or...

  2. 40 CFR 264.174 - Inspections.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Containers § 264.174 Inspections. At least weekly, the owner or operator must inspect areas where containers are stored, except for Performance Track member facilities, that may conduct inspections at least once... Track member facility must follow the procedures identified in § 264.15(b)(5) of this part. The owner or...

  3. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  4. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  5. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  6. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  7. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  8. 21 CFR 26.4 - Product coverage.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... Products regulated by the Food and Drug Administration's Center for Biologics Evaluation and Research or... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Product coverage. 26.4 Section 26.4 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL MUTUAL RECOGNITION OF...

  9. 21 CFR 26.4 - Product coverage.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... Products regulated by the Food and Drug Administration's Center for Biologics Evaluation and Research or... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Product coverage. 26.4 Section 26.4 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL MUTUAL RECOGNITION OF...

  10. 34 CFR 75.264 - Transfers among budget categories.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 1 2014-07-01 2014-07-01 false Transfers among budget categories. 75.264 Section 75... Made Miscellaneous § 75.264 Transfers among budget categories. A grantee may, notwithstanding any... these transfers. (Authority: 20 U.S.C. 1221e-3 and 3474) [62 FR 40425, July 28, 1997] ...

  11. 37 CFR 2.64 - Final action.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Final action. 2.64 Section 2... COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Examination of Application and Action by Applicants § 2.64 Final action. (a) On the first or any subsequent reexamination or reconsideration the refusal of the...

  12. 37 CFR 2.64 - Final action.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2011-07-01 2011-07-01 false Final action. 2.64 Section 2... COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Examination of Application and Action by Applicants § 2.64 Final action. (a) On the first or any subsequent reexamination or reconsideration the refusal of the...

  13. 40 CFR 264.13 - General waste analysis.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false General waste analysis. 264.13 Section... Facility Standards § 264.13 General waste analysis. (a)(1) Before an owner or operator treats, stores, or... detailed chemical and physical analysis of a representative sample of the wastes. At a minimum, the...

  14. 40 CFR 264.53 - Copies of contingency plan.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Copies of contingency plan. 264.53... Contingency Plan and Emergency Procedures § 264.53 Copies of contingency plan. A copy of the contingency plan... called upon to provide emergency services. [Comment: The contingency plan must be submitted to the...

  15. 20 CFR 416.264 - When does the special SSI eligibility status apply.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false When does the special SSI eligibility status apply. 416.264 Section 416.264 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A...

  16. 20 CFR 416.264 - When does the special SSI eligibility status apply.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false When does the special SSI eligibility status apply. 416.264 Section 416.264 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A...

  17. 20 CFR 416.264 - When does the special SSI eligibility status apply.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false When does the special SSI eligibility status apply. 416.264 Section 416.264 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Special Provisions for People Who Work Despite A...

  18. 40 CFR 264.144 - Cost estimate for post-closure care.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... § 264.145. For owners or operators using the financial test or corporate guarantee, the post-closure... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Cost estimate for post-closure care... FACILITIES Financial Requirements § 264.144 Cost estimate for post-closure care. (a) The owner or operator of...

  19. 40 CFR 264.144 - Cost estimate for post-closure care.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... § 264.145. For owners or operators using the financial test or corporate guarantee, the post-closure... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Cost estimate for post-closure care... FACILITIES Financial Requirements § 264.144 Cost estimate for post-closure care. (a) The owner or operator of...

  20. 40 CFR 264.144 - Cost estimate for post-closure care.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... § 264.145. For owners or operators using the financial test or corporate guarantee, the post-closure... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Cost estimate for post-closure care... FACILITIES Financial Requirements § 264.144 Cost estimate for post-closure care. (a) The owner or operator of...

  1. 40 CFR 264.144 - Cost estimate for post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... § 264.145. For owners or operators using the financial test or corporate guarantee, the post-closure... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Cost estimate for post-closure care... FACILITIES Financial Requirements § 264.144 Cost estimate for post-closure care. (a) The owner or operator of...

  2. 40 CFR 264.145 - Financial assurance for post-closure care.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... in accordance with § 264.145(i). (f) Financial test and corporate guarantee for post-closure care. (1... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Financial assurance for post-closure... DISPOSAL FACILITIES Financial Requirements § 264.145 Financial assurance for post-closure care. The owner...

  3. 40 CFR 264.257 - Special requirements for incompatible wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... DISPOSAL FACILITIES Waste Piles § 264.257 Special requirements for incompatible wastes. (a) Incompatible... placed in the same pile, unless § 264.17(b) is complied with. (b) A pile of hazardous waste that is incompatible with any waste or other material stored nearby in containers, other piles, open tanks, or surface...

  4. 76 FR 28675 - Spirotetramat; Pesticide Tolerances

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    ...; vegetables, legume, group 06 (except soybean) at 4 ppm; plum, prune, dried at 4.5 ppm; vegetables, foliage of legume, except soybean, subgroup 07A at 5 ppm; cotton, gin byproducts at 7 ppm; soybean at 4 ppm; soybean..., 264-1050, 264-1051, 264-1065), on cotton; soybeans; vegetable, legume, crop group 6; acerola; atemoya...

  5. 40 CFR 264.145 - Financial assurance for post-closure care.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in accordance with § 264.145(i). (f) Financial test and corporate guarantee for post-closure care. (1... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Financial assurance for post-closure... DISPOSAL FACILITIES Financial Requirements § 264.145 Financial assurance for post-closure care. The owner...

  6. 40 CFR 264.145 - Financial assurance for post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... in accordance with § 264.145(i). (f) Financial test and corporate guarantee for post-closure care. (1... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Financial assurance for post-closure... DISPOSAL FACILITIES Financial Requirements § 264.145 Financial assurance for post-closure care. The owner...

  7. 40 CFR 264.151 - Wording of the instruments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... assurance for closure or post-closure care is demonstrated through the financial test specified in subpart H... parts 264 and 265. The current closure and/or post-closure cost estimates covered by such a test are... CFR parts 264 and 265. The current closure and/or post-closure cost estimate covered by the test are...

  8. 40 CFR 264.145 - Financial assurance for post-closure care.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... in accordance with § 264.145(i). (f) Financial test and corporate guarantee for post-closure care. (1... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Financial assurance for post-closure... DISPOSAL FACILITIES Financial Requirements § 264.145 Financial assurance for post-closure care. The owner...

  9. 40 CFR 264.151 - Wording of the instruments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... assurance for closure or post-closure care is demonstrated through the financial test specified in subpart H... parts 264 and 265. The current closure and/or post-closure cost estimates covered by such a test are... CFR parts 264 and 265. The current closure and/or post-closure cost estimate covered by the test are...

  10. 40 CFR 264.151 - Wording of the instruments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... assurance for closure or post-closure care is demonstrated through the financial test specified in subpart H... parts 264 and 265. The current closure and/or post-closure cost estimates covered by such a test are... CFR parts 264 and 265. The current closure and/or post-closure cost estimate covered by the test are...

  11. 40 CFR 264.145 - Financial assurance for post-closure care.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... in accordance with § 264.145(i). (f) Financial test and corporate guarantee for post-closure care. (1... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Financial assurance for post-closure... DISPOSAL FACILITIES Financial Requirements § 264.145 Financial assurance for post-closure care. The owner...

  12. 40 CFR 264.35 - Required aisle space.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Required aisle space. 264.35 Section... Preparedness and Prevention § 264.35 Required aisle space. The owner or operator must maintain aisle space to... Regional Administrator that aisle space is not needed for any of these purposes. [Comment: Part 270 of this...

  13. 40 CFR 264.35 - Required aisle space.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Required aisle space. 264.35 Section... Preparedness and Prevention § 264.35 Required aisle space. The owner or operator must maintain aisle space to... Regional Administrator that aisle space is not needed for any of these purposes. [Comment: Part 270 of this...

  14. 40 CFR 264.35 - Required aisle space.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Required aisle space. 264.35 Section... Preparedness and Prevention § 264.35 Required aisle space. The owner or operator must maintain aisle space to... Regional Administrator that aisle space is not needed for any of these purposes. [Comment: Part 270 of this...

  15. 40 CFR 264.35 - Required aisle space.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Required aisle space. 264.35 Section... Preparedness and Prevention § 264.35 Required aisle space. The owner or operator must maintain aisle space to... Regional Administrator that aisle space is not needed for any of these purposes. [Comment: Part 270 of this...

  16. 40 CFR 264.35 - Required aisle space.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Required aisle space. 264.35 Section... Preparedness and Prevention § 264.35 Required aisle space. The owner or operator must maintain aisle space to... Regional Administrator that aisle space is not needed for any of these purposes. [Comment: Part 270 of this...

  17. 40 CFR 264.1064 - Recordkeeping requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... facility (e.g., identify the hazardous waste management unit on a facility plot plan). (iii) Type of... schedule as specified in § 264.1033(a)(2). (3) Where an owner or operator chooses to use test data to... device, a performance test plan as specified in § 264.1035(b)(3). (4) Documentation of compliance with...

  18. 40 CFR 264.1064 - Recordkeeping requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... facility (e.g., identify the hazardous waste management unit on a facility plot plan). (iii) Type of... schedule as specified in § 264.1033(a)(2). (3) Where an owner or operator chooses to use test data to... device, a performance test plan as specified in § 264.1035(b)(3). (4) Documentation of compliance with...

  19. 40 CFR 264.1064 - Recordkeeping requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... facility (e.g., identify the hazardous waste management unit on a facility plot plan). (iii) Type of... schedule as specified in § 264.1033(a)(2). (3) Where an owner or operator chooses to use test data to... device, a performance test plan as specified in § 264.1035(b)(3). (4) Documentation of compliance with...

  20. 40 CFR 264.1064 - Recordkeeping requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... facility (e.g., identify the hazardous waste management unit on a facility plot plan). (iii) Type of... schedule as specified in § 264.1033(a)(2). (3) Where an owner or operator chooses to use test data to... device, a performance test plan as specified in § 264.1035(b)(3). (4) Documentation of compliance with...

  1. 49 CFR 195.264 - Impoundment, protection against entry, normal/emergency venting or pressure/vacuum relief for...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... devices must be provided for each low-pressure and high-pressure breakout tank. (e) For normal/emergency... and vacuum-relieving devices installed on high pressure tanks built to API Standard 2510 (incorporated.../emergency venting or pressure/vacuum relief for aboveground breakout tanks. 195.264 Section 195.264...

  2. 42 CFR 2.64 - Procedures and criteria for orders authorizing disclosures for noncriminal purposes.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Procedures and criteria for orders authorizing disclosures for noncriminal purposes. 2.64 Section 2.64 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS...

  3. 42 CFR 2.64 - Procedures and criteria for orders authorizing disclosures for noncriminal purposes.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Procedures and criteria for orders authorizing disclosures for noncriminal purposes. 2.64 Section 2.64 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS...

  4. 42 CFR 2.64 - Procedures and criteria for orders authorizing disclosures for noncriminal purposes.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Procedures and criteria for orders authorizing disclosures for noncriminal purposes. 2.64 Section 2.64 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS...

  5. 42 CFR 2.64 - Procedures and criteria for orders authorizing disclosures for noncriminal purposes.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Procedures and criteria for orders authorizing disclosures for noncriminal purposes. 2.64 Section 2.64 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS...

  6. 42 CFR 2.64 - Procedures and criteria for orders authorizing disclosures for noncriminal purposes.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Procedures and criteria for orders authorizing disclosures for noncriminal purposes. 2.64 Section 2.64 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS CONFIDENTIALITY OF ALCOHOL AND DRUG ABUSE PATIENT RECORDS...

  7. 40 CFR 264.53 - Copies of contingency plan.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Copies of contingency plan. 264.53... Contingency Plan and Emergency Procedures § 264.53 Copies of contingency plan. A copy of the contingency plan and all revisions to the plan must be: (a) Maintained at the facility; and (b) Submitted to all local...

  8. 40 CFR 264.53 - Copies of contingency plan.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Copies of contingency plan. 264.53... Contingency Plan and Emergency Procedures § 264.53 Copies of contingency plan. A copy of the contingency plan and all revisions to the plan must be: (a) Maintained at the facility; and (b) Submitted to all local...

  9. 40 CFR 264.53 - Copies of contingency plan.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Copies of contingency plan. 264.53... Contingency Plan and Emergency Procedures § 264.53 Copies of contingency plan. A copy of the contingency plan and all revisions to the plan must be: (a) Maintained at the facility; and (b) Submitted to all local...

  10. 40 CFR 264.53 - Copies of contingency plan.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Copies of contingency plan. 264.53... Contingency Plan and Emergency Procedures § 264.53 Copies of contingency plan. A copy of the contingency plan and all revisions to the plan must be: (a) Maintained at the facility; and (b) Submitted to all local...

  11. 40 CFR 264.1033 - Standards: Closed-vent systems and control devices.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Standards: Closed-vent systems and control devices. 264.1033 Section 264.1033 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... no greater than 20 per-cent of the time required to con-sume the total carbon working cap-a-city...

  12. 20 CFR 667.264 - What other activities are prohibited under title I of WIA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false What other activities are prohibited under title I of WIA? 667.264 Section 667.264 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION...) WIA title I funds must not be spent on: (1) The wages of incumbent employees during their...

  13. 28 CFR 115.264 - Staff first responder duties.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Staff first responder duties. 115.264... Report § 115.264 Staff first responder duties. (a) Upon learning of an allegation that a resident was sexually abused, the first security staff member to respond to the report shall be required to: (1...

  14. 28 CFR 115.264 - Staff first responder duties.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Staff first responder duties. 115.264... Report § 115.264 Staff first responder duties. (a) Upon learning of an allegation that a resident was sexually abused, the first security staff member to respond to the report shall be required to: (1...

  15. 28 CFR 115.264 - Staff first responder duties.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Staff first responder duties. 115.264... Report § 115.264 Staff first responder duties. (a) Upon learning of an allegation that a resident was sexually abused, the first security staff member to respond to the report shall be required to: (1...

  16. Improved lossless intra coding for H.264/MPEG-4 AVC.

    PubMed

    Lee, Yung-Lyul; Han, Ki-Hun; Sullivan, Gary J

    2006-09-01

    A new lossless intra coding method based on sample-by-sample differential pulse code modulation (DPCM) is presented as an enhancement of the H.264/MPEG-4 AVC standard. The H.264/AVC design includes a multidirectional spatial prediction method to reduce spatial redundancy by using neighboring samples as a prediction for the samples in a block of data to be encoded. In the new lossless intra coding method, the spatial prediction is performed based on samplewise DPCM instead of in the block-based manner used in the current H.264/AVC standard, while the block structure is retained for the residual difference entropy coding process. We show that the new method, based on samplewise DPCM, does not have a major complexity penalty, despite its apparent pipeline dependencies. Experiments show that the new lossless intra coding method reduces the bit rate by approximately 12% in comparison with the lossless intra coding method previously included in the H.264/AVC standard. As a result, the new method is currently being adopted into the H.264/AVC standard in a new enhancement project.

  17. Comparison of RAW264.7, human whole blood and PBMC assays to screen for immunomodulators.

    PubMed

    Elisia, Ingrid; Pae, Han Bee; Lam, Vivian; Cederberg, Rachel; Hofs, Elyse; Krystal, Gerald

    2018-01-01

    The RAW264.7 mouse macrophage cell line is used extensively to carry out in vitro screens for immunomodulators. Compounds that are effective at reducing the expression of pro-inflammatory cytokines or nitric oxide (NO) from lipopolysaccharide (LPS)-stimulated RAW264.7 cells are often considered candidate anti-inflammatory agents for humans. There is, however, very little data on the reliability of this screen to identify bona fide human immunomodulators. We compared the efficacy of 37 purported immunomodulators to modulate LPS or E. coli-induced inflammatory responses in RAW264.7 cell, whole human blood and human peripheral blood mononuclear cell (PBMC) assays. Interestingly, there was no significant correlation (R=0.315) between the responses obtained with RAW264.7 cells and the whole blood assay (WBA), suggesting that compounds demonstrating efficacy in RAW264.7 cells may be ineffective in humans, and, more importantly, compounds that are effective in humans may be missed with a RAW264.7 screen. Interestingly, there was also no significant correlation between the WBA and human PBMCs when the latter were cultured with 10% FCS, but a moderate correlation was seen when the PBMCs were cultured with 25% autologous plasma. The presence of plasma thus contributes to the overall inflammatory response observed in the WBA. We then asked if RAW264.7 cells, given that they are mouse macrophage-like cells, respond in a manner similar to primary murine derived macrophages. Intriguingly, there was no significant correlation (R=0.012) with the 37 putative immunomodulators, pointing to distinct inflammatory response mechanisms in the two model systems. We conclude that the use of a WBA to confirm potential immunomodulators obtained from high throughput screening with RAW264.7 cells is advisable and that future screens be carried out using a WBA. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Possibilities of production of transfermium nuclei in complete fusion reactions with radioactive beams

    NASA Astrophysics Data System (ADS)

    Hong, Juhee; Adamian, G. G.; Antonenko, N. V.

    2017-07-01

    The possibilities of direct production of new isotopes of transfermium nuclei 261,263,264No, 264Lr263, 263,264,266,268Rf, 265Db264, and 267,268,270,272Sg are studied in various asymmetric hot fusion-evaporation reactions with radioactive beams. The optimal reaction partners and conditions for the synthesis of new isotopes are suggested. The products of the suggested reactions can fill a gap of unknown isotopes between the isotopes of heaviest nuclei obtained in the x n evaporation channels of the cold and hot complete fusion reactions with the stable beams.

  19. [Zinc-dependent metalloprotease 1 promotes apoptosis of RAW264.7 macrophages].

    PubMed

    Li, Peng; He, Yonglin; Zhang, Jiming; Fang, Chencheng

    2015-12-01

    To construct the eukaryotic expression vector of zinc-dependent metalloprotease 1 (zmp1) gene from Bacillus Calmette-Guerin (BCG) and investigate its impact on the apoptosis of RAW264.7 macrophages. Zmp1 gene was amplified from the genome of BCG by PCR. The zmp1 gene fragment was inserted into multiple cloning sites of pEGFP-N1 to construct the eukaryotic expression vector pEGFP-N1-zmp1. The constructed pEGFP-N1-zmp1 was transfected into RAW264.7 cells by Lipofectamine(TM) 2000. The expression of green fluorescent protein (GFP) was observed by fluorescence microscopy. The zmp1 mRNA was detected by quantitative real-time PCR (qR-PCR). The effect of Zmp1 protein on the apoptosis of RAW264.7 macrophages was detected by flow cytometry (FCM). With zmp1 gene amplified by PCR, we successfully constructed the recombinant vector pEGFP-N1-zmp1 as demonstrated by restriction enzyme analysis and sequencing. GFP was seen in RAW264.7 cells 24 hours after transfected with the recombinant plasmid. As qRT-PCR showed, the expression level of zmp1 mRNA was up-regulated. The early apoptotic rate increased 48 hours after transfection. The increased expression of Zmp1 in RAW264.7 cells promotes the apoptosis of RAW264.7 cells.

  20. 40 CFR 264.146 - Use of a mechanism for financial assurance of both closure and post-closure care.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... assurance of both closure and post-closure care. 264.146 Section 264.146 Protection of Environment... mechanism for financial assurance of both closure and post-closure care. An owner or operator may satisfy the requirements for financial assurance for both closure and post-closure care for one or more...

  1. 41 CFR 301-10.264 - What amount must the Government be reimbursed for travel on Government aircraft?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... regularly scheduled commercial airline service and are authorized to use Government aircraft, you do not have to reimburse the Government. (c) For political travel on a Government aircraft (i.e., for any trip... Government be reimbursed for travel on Government aircraft? 301-10.264 Section 301-10.264 Public Contracts...

  2. 41 CFR 301-10.264 - What amount must the Government be reimbursed for travel on Government aircraft?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... regularly scheduled commercial airline service and are authorized to use Government aircraft, you do not have to reimburse the Government. (c) For political travel on a Government aircraft (i.e., for any trip... Government be reimbursed for travel on Government aircraft? 301-10.264 Section 301-10.264 Public Contracts...

  3. 8 CFR 264.2 - Application for creation of record of permanent residence.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... permanent residence. 264.2 Section 264.2 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION... record of permanent residence. (a) Jurisdiction. An applicant who believes that he/she is eligible for presumption of lawful admission for permanent residence under § 101.1 or § 101.2 of this chapter or for lawful...

  4. 12 CFR 264b.5 - Gifts of more than minimal value.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Gifts of more than minimal value. 264b.5 Section 264b.5 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL... entirely outside the United States (such as transportation, food, and lodging) of more than minimal value...

  5. 40 CFR 264.228 - Closure and post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Closure and post-closure care. 264.228... Surface Impoundments § 264.228 Closure and post-closure care. (a) At closure, the owner or operator must... materials are left in place at final closure, the owner or operator must comply with all post-closure...

  6. 40 CFR 264.228 - Closure and post-closure care.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Closure and post-closure care. 264.228... Surface Impoundments § 264.228 Closure and post-closure care. (a) At closure, the owner or operator must... materials are left in place at final closure, the owner or operator must comply with all post-closure...

  7. 40 CFR 264.228 - Closure and post-closure care.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Closure and post-closure care. 264.228... Surface Impoundments § 264.228 Closure and post-closure care. (a) At closure, the owner or operator must... materials are left in place at final closure, the owner or operator must comply with all post-closure...

  8. 40 CFR 264.228 - Closure and post-closure care.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Closure and post-closure care. 264.228... Surface Impoundments § 264.228 Closure and post-closure care. (a) At closure, the owner or operator must... materials are left in place at final closure, the owner or operator must comply with all post-closure...

  9. 40 CFR 264.228 - Closure and post-closure care.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Closure and post-closure care. 264.228... Surface Impoundments § 264.228 Closure and post-closure care. (a) At closure, the owner or operator must... materials are left in place at final closure, the owner or operator must comply with all post-closure...

  10. 40 CFR 264.1057 - Standards: Valves in gas/vapor service or in light liquid -service.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...: Valves in gas/vapor service or in light liquid -service. (a) Each valve in gas/vapor or light liquid... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Standards: Valves in gas/vapor service or in light liquid -service. 264.1057 Section 264.1057 Protection of Environment ENVIRONMENTAL...

  11. 40 CFR 264.1057 - Standards: Valves in gas/vapor service or in light liquid -service.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...: Valves in gas/vapor service or in light liquid -service. (a) Each valve in gas/vapor or light liquid... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Standards: Valves in gas/vapor service or in light liquid -service. 264.1057 Section 264.1057 Protection of Environment ENVIRONMENTAL...

  12. 40 CFR 264.1057 - Standards: Valves in gas/vapor service or in light liquid -service.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...: Valves in gas/vapor service or in light liquid -service. (a) Each valve in gas/vapor or light liquid... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Standards: Valves in gas/vapor service or in light liquid -service. 264.1057 Section 264.1057 Protection of Environment ENVIRONMENTAL...

  13. 40 CFR 264.1057 - Standards: Valves in gas/vapor service or in light liquid -service.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...: Valves in gas/vapor service or in light liquid -service. (a) Each valve in gas/vapor or light liquid... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Standards: Valves in gas/vapor service or in light liquid -service. 264.1057 Section 264.1057 Protection of Environment ENVIRONMENTAL...

  14. 40 CFR 264.1057 - Standards: Valves in gas/vapor service or in light liquid -service.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...: Valves in gas/vapor service or in light liquid -service. (a) Each valve in gas/vapor or light liquid... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Standards: Valves in gas/vapor service or in light liquid -service. 264.1057 Section 264.1057 Protection of Environment ENVIRONMENTAL...

  15. [Chrysin inhibits lipopolysaccharide-induced inflammatory responses of macrophages via JAK-STATs signaling pathway].

    PubMed

    Qi, Shi-Mei; Li, Qiang; Jiang, Qi; Qi, Zhi-Lin; Zhang, Yao

    2018-03-20

    To investigate the mechanism of chrysin in regulating lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells. RAW264.7 cells were treated with different concentrations (0, 5, 10, 20, 40, 60, 80, 100, 150, and 200 µg/mL) of chrysin for 24 h, and the cell viability was measured using CCK-8. RAW264.7 cells were pre-treated with 10, 30, or 60 µg/mL chrysin for 2 h before stimulation with LPS for different times. The levels of TNF-α, IL-6 and MCP-1 were detected by ELISA, and Western blotting was used to detect the phosphorylation of JAK- 1, JAK-2, STAT-1 and STAT-3. The level of reactive oxygen species in RAW264.7 cells was detected by CM-H2DCFDA fluorescence probe. The effect of ROS on LPS-induced JAK-STATs signal and the inflammatory response of RAW264.7 cells was detected by ROS scavenger NAC. The transcription factors STAT-1 and STAT-3 nuclear translocation were observed by laser confocal microscopy. Chrysin below 60 µg/mL did not significantly affect the viability of RAW264.7 cells. At 10, 30, and 60 µg/mL, chrysin dose-dependently inhibited the expression of iNOS induced by LPS. Chrysin treatment also inhibited LPS-induced phosphorylation of JAK-STATs, nuclear translocation of STAT1 and STAT3, release of TNF-α, IL-6 and MCP-1, and the production of ROS in RAW264.7 cells; ROS acted as an upstream signal to mediate the activation of JAK-STATs signaling pathway. Chrysin blocks the activity of JAK-STATs mediated by ROS to inhibit LPS-induced inflammatory response in RAW264.7 cells.

  16. Activation of AMPA receptor promotes TNF-α release via the ROS-cSrc-NFκB signaling cascade in RAW264.7 macrophages

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Cheng, Xiu-Li; Ding, Fan; Li, Hui

    2015-05-29

    The relationship between glutamate signaling and inflammation has not been well defined. This study aimed to investigate the role of AMPA receptor (AMPAR) in the expression and release of tumor necrosis factor-alpha (TNF-α) from macrophages and the underlying mechanisms. A series of approaches, including confocal microscopy, immunofluorescency, flow cytometry, ELISA and Western blotting, were used to estimate the expression of AMPAR and downstream signaling molecules, TNF-α release and reactive oxygen species (ROS) generation in the macrophage-like RAW264.7 cells. The results demonstrated that AMPAR was expressed in RAW264.7 cells. AMPA significantly enhanced TNF-α release from RAW264.7 cells, and this effect wasmore » abolished by CNQX (AMPAR antagonist). AMPA also induced elevation of ROS production, phosphorylation of c-Src and activation of nuclear factor (NF)-κB in RAW264.7 cells. Blocking c-Src by PP2, scavenging ROS by glutathione (GSH) or inhibiting NF-κB activation by pyrrolidine dithiocarbamate (PDTC) decreased TNF-α production from RAW264.7 cells. We concluded that AMPA promotes TNF-α release in RAW264.7 macrophages likely through the following signaling cascade: AMPAR activation → ROS generation → c-Src phosphorylation → NF-κB activation → TNF-α elevation. The study suggests that AMPAR may participate in macrophage activation and inflammation. - Highlights: • AMPAR is expressed in RAW264.7 macrophages and is upregulated by AMPA stimulation. • Activation of AMPAR stimulates TNF-α release in macrophages through the ROS-cSrc-NFκB signaling cascade. • Macrophage AMPAR signaling may play an important role in inflammation.« less

  17. Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer.

    PubMed

    Funston, Scott J; Tsaousi, Konstantina; Rudden, Michelle; Smyth, Thomas J; Stevenson, Paul S; Marchant, Roger; Banat, Ibrahim M

    2016-09-01

    Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, incubation at the lower temperature resulted in consistently higher concentration of RL production throughout the growth period. After 264 h, the concentration of crude RL extract for the 25 °C culture was 2.79 g/l compared to 1.99 g/l for the 30 °C culture. Overall RL production concentration after 264 h was 0.258 g/g dry cell biomass (DCB) for the 30 °C culture compared to 0.587 g/g DCB for the 25 °C culture. Real-time PCR (qPCR) was also used to analyse expression of the RL biosynthesis genes throughout the incubation period at 25 °C showing that the expression of the rhlA, rhlB and rhlC genes is continuous. During the log and early stationary phases of growth, expression levels remain low and are increased upon entry to the late stationary phase. B. thailandensis E264 produces mostly di-RLs and the Di-RL C14-C14 in most abundance (41.88 %). Fermentations were also carried out in small-scale bioreactors (4 l working volume) under controlled conditions, and results showed that RL production was maintained. Our findings show that B. thailandensis E264 has excellent potential for industrial scale RL production.

  18. TAK-264 (MLN0264) in Previously Treated Asian Patients with Advanced Gastrointestinal Carcinoma Expressing Guanylyl Cyclase C: Results from an Open-Label, Non-randomized Phase 1 Study

    PubMed Central

    Bang, Yung-Jue; Takano, Toshimi; Lin, Chia-Chi; Fasanmade, Adedigbo; Yang, Huyuan; Danaee, Hadi; Asato, Takayuki; Kalebic, Thea; Wang, Hui; Doi, Toshihiko

    2018-01-01

    Purpose This phase 1 dose-escalation portion of the study evaluated the safety, pharmacokinetics (PK), and antitumor activity of TAK-264 in Asian patients with advanced gastrointestinal (GI) carcinoma or metastatic or recurrent gastric or gastroesophageal junction adenocarcinoma expressing guanylyl cyclase C (GCC). Materials and Methods Adult patients with advanced GI malignancies expressing GCC (H-score ≥ 10) received TAK-264 on day 1 of 3-week cycles as 30-minute intravenous infusions for up to 1 year or until disease progression or unacceptable toxicity. The primary objectives were to evaluate the safety profile including dose-limiting toxicities (DLTs) during cycle 1, determine the maximum tolerated dose (MTD), and characterize the PK profile of TAK-264. Results Twelve patients were enrolled and treated with 1.2 mg/kg (n=3), 1.5 mg/kg (n=3), or 1.8 mg/kg TAK-264 (n=6). Median number of treatment cycles received was two (range, 1 to 10). None of the patients experienced a DLT and the MTD was not determined. Ten patients (83%) experienced adverse events (AEs). The most common were neutropenia, anorexia, and nausea (each reported by four patients). Five patients (42%) experienced grade ≥ 3 AEs consisting of tumor hemorrhage and hypertension, ascites, adrenal insufficiency, neutropenia and asthenia. Serum exposure to TAK-264 increased proportionally with the dose and the median half-life was approximately 5.5-6.6 days. No patients experienced an objective response. Conclusion TAK-264 demonstrated a manageable safety profile with limited antitumor activity consistent with studies conducted in Western patients with advanced GI malignancies. TAK-264 exposure increased proportionally with the dose. PMID:28494535

  19. Separation and purification of bioactive botrallin and TMC-264 by a combination of HSCCC and semi-preparative HPLC from endophytic fungus Hyalodendriella sp. Ponipodef12.

    PubMed

    Mao, Ziling; Luo, Ruiya; Luo, Haiyu; Tian, Jin; Liu, Hongwei; Yue, Yang; Wang, Mingan; Peng, Youliang; Zhou, Ligang

    2014-09-01

    Two dibenzo-α-pyrones, botrallin (1) and TMC-264 (2) were preparatively separated from crude ethyl acetate extract of the endophytic fungus Hyalodendriella sp. Ponipodef12, which was isolated from the hybrid 'Neva' of Populus deltoides Marsh × P. nigra L. using a combination of high-speed counter-current chromatography (HSCCC) and semi-preparative HPLC. Botrallin (1) with 74.73% of purity and TMC-264 (2) with 82.29% of purity were obtained through HSCCC by employing a solvent system containing n-hexane-ethyl acetate-methanol-water at a volume ratio of 1.2:1.0:0.9:1.0. It was the first time for TMC-264 (2) to be isolated from this fungus. TMC-264 (2) showed strong antimicrobial and antinematodal activity, and botrallin (1) exhibited moderate inhibitory activity on acetylcholinesterase.

  20. 40 CFR 264.1033 - Standards: Closed-vent systems and control devices.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... § 264.1035(b)(4)(iii)(F). (h) An owner or operator using a carbon adsorption system such as a carbon...). (iii) A steam-assisted or nonassisted flare designed for and operated with an exit velocity, as... established as a requirement of § 264.1035(b)(4)(iii)(G), whichever is longer. (2) Replace the existing carbon...

  1. 40 CFR 264.1033 - Standards: Closed-vent systems and control devices.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... § 264.1035(b)(4)(iii)(F). (h) An owner or operator using a carbon adsorption system such as a carbon...). (iii) A steam-assisted or nonassisted flare designed for and operated with an exit velocity, as... established as a requirement of § 264.1035(b)(4)(iii)(G), whichever is longer. (2) Replace the existing carbon...

  2. 40 CFR 264.1033 - Standards: Closed-vent systems and control devices.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... § 264.1035(b)(4)(iii)(F). (h) An owner or operator using a carbon adsorption system such as a carbon...). (iii) A steam-assisted or nonassisted flare designed for and operated with an exit velocity, as... established as a requirement of § 264.1035(b)(4)(iii)(G), whichever is longer. (2) Replace the existing carbon...

  3. 40 CFR 264.555 - Disposal of CAMU-eligible wastes in permitted hazardous waste landfills.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... taking place identifies principal hazardous constitutes in such waste, in accordance with § 264.552(e)(4... following standards specified for CAMU-eligible wastes: (i) The treatment standards under § 264.552(e)(4)(iv... authorizes receipt of such waste. (e) For each remediation, CAMU-eligible waste may not be placed in an off...

  4. 40 CFR 264.555 - Disposal of CAMU-eligible wastes in permitted hazardous waste landfills.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... taking place identifies principal hazardous constitutes in such waste, in accordance with § 264.552(e)(4... following standards specified for CAMU-eligible wastes: (i) The treatment standards under § 264.552(e)(4)(iv... authorizes receipt of such waste. (e) For each remediation, CAMU-eligible waste may not be placed in an off...

  5. 40 CFR 264.555 - Disposal of CAMU-eligible wastes in permitted hazardous waste landfills.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... taking place identifies principal hazardous constitutes in such waste, in accordance with § 264.552(e)(4... following standards specified for CAMU-eligible wastes: (i) The treatment standards under § 264.552(e)(4)(iv... authorizes receipt of such waste. (e) For each remediation, CAMU-eligible waste may not be placed in an off...

  6. 40 CFR 264.555 - Disposal of CAMU-eligible wastes in permitted hazardous waste landfills.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... taking place identifies principal hazardous constitutes in such waste, in accordance with § 264.552(e)(4... following standards specified for CAMU-eligible wastes: (i) The treatment standards under § 264.552(e)(4)(iv... authorizes receipt of such waste. (e) For each remediation, CAMU-eligible waste may not be placed in an off...

  7. 40 CFR 264.1062 - Alternative standards for valves in gas/vapor service or in light liquid service: skip period...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... gas/vapor service or in light liquid service: skip period leak detection and repair. 264.1062 Section... Emission Standards for Equipment Leaks § 264.1062 Alternative standards for valves in gas/vapor service or in light liquid service: skip period leak detection and repair. (a) An owner or operator subject to...

  8. 40 CFR 264.1062 - Alternative standards for valves in gas/vapor service or in light liquid service: skip period...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... gas/vapor service or in light liquid service: skip period leak detection and repair. 264.1062 Section... Emission Standards for Equipment Leaks § 264.1062 Alternative standards for valves in gas/vapor service or in light liquid service: skip period leak detection and repair. (a) An owner or operator subject to...

  9. 40 CFR 264.1062 - Alternative standards for valves in gas/vapor service or in light liquid service: skip period...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... gas/vapor service or in light liquid service: skip period leak detection and repair. 264.1062 Section... Emission Standards for Equipment Leaks § 264.1062 Alternative standards for valves in gas/vapor service or in light liquid service: skip period leak detection and repair. (a) An owner or operator subject to...

  10. 40 CFR 264.1061 - Alternative standards for valves in gas/vapor service or in light liquid service: percentage of...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... gas/vapor service or in light liquid service: percentage of valves allowed to leak. 264.1061 Section... Emission Standards for Equipment Leaks § 264.1061 Alternative standards for valves in gas/vapor service or in light liquid service: percentage of valves allowed to leak. (a) An owner or operator subject to...

  11. 40 CFR 264.1061 - Alternative standards for valves in gas/vapor service or in light liquid service: percentage of...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... gas/vapor service or in light liquid service: percentage of valves allowed to leak. 264.1061 Section... Emission Standards for Equipment Leaks § 264.1061 Alternative standards for valves in gas/vapor service or in light liquid service: percentage of valves allowed to leak. (a) An owner or operator subject to...

  12. 40 CFR 264.1061 - Alternative standards for valves in gas/vapor service or in light liquid service: percentage of...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... gas/vapor service or in light liquid service: percentage of valves allowed to leak. 264.1061 Section... Emission Standards for Equipment Leaks § 264.1061 Alternative standards for valves in gas/vapor service or in light liquid service: percentage of valves allowed to leak. (a) An owner or operator subject to...

  13. 40 CFR 264.1061 - Alternative standards for valves in gas/vapor service or in light liquid service: percentage of...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... gas/vapor service or in light liquid service: percentage of valves allowed to leak. 264.1061 Section... Emission Standards for Equipment Leaks § 264.1061 Alternative standards for valves in gas/vapor service or in light liquid service: percentage of valves allowed to leak. (a) An owner or operator subject to...

  14. 40 CFR 264.1062 - Alternative standards for valves in gas/vapor service or in light liquid service: skip period...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... gas/vapor service or in light liquid service: skip period leak detection and repair. 264.1062 Section... Emission Standards for Equipment Leaks § 264.1062 Alternative standards for valves in gas/vapor service or in light liquid service: skip period leak detection and repair. (a) An owner or operator subject to...

  15. 40 CFR 264.1061 - Alternative standards for valves in gas/vapor service or in light liquid service: percentage of...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... gas/vapor service or in light liquid service: percentage of valves allowed to leak. 264.1061 Section... Emission Standards for Equipment Leaks § 264.1061 Alternative standards for valves in gas/vapor service or in light liquid service: percentage of valves allowed to leak. (a) An owner or operator subject to...

  16. 40 CFR 264.1062 - Alternative standards for valves in gas/vapor service or in light liquid service: skip period...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... gas/vapor service or in light liquid service: skip period leak detection and repair. 264.1062 Section... Emission Standards for Equipment Leaks § 264.1062 Alternative standards for valves in gas/vapor service or in light liquid service: skip period leak detection and repair. (a) An owner or operator subject to...

  17. Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway.

    PubMed

    Cheng, Feier; Yan, Xiaoyan; Zhang, Miaoqing; Chang, Mingchang; Yun, Shaojun; Meng, Junlong; Liu, Jingyu; Feng, Cui-Ping

    2017-04-19

    Agaricus blazei Murill (ABM) is a common anticancer folk remedy. Its active ingredients, i.e., polysaccharides, have been isolated and exhibit indirect tumor-suppressing activity via immunological activation. The effects of polysaccharides derived from A. blazei Murill (ABMP) on RAW 264.7 cells were examined by western blotting and real-time reverse transcription polymerase chain reaction (RT-PCR). The effects of 500, 1000, and 2000 μg mL -1 ABMP on the growth of RAW 264.7 cells were evaluated by measuring the OD 490 value; the optimum concentration was found to be 1000 μg mL -1 . Based on the RT-PCR results, the expression levels of JNK, ERK, and p38 decreased substantially in lipopolysaccharide (LPS)-induced RAW 264.7 cells treated with ABMP. In RAW 264.7 cells treated with LPS, the protein expression levels of JNK, ERK, and p38 were decreased, as were the levels of phosphorylated JNK, ERK, and p38. These results indicate that the MAPK signal transduction pathway is a potential mechanism by which ABMP regulates the cell-mediated immunity of RAW 264.7 cells.

  18. Video traffic characteristics of modern encoding standards: H.264/AVC with SVC and MVC extensions and H.265/HEVC.

    PubMed

    Seeling, Patrick; Reisslein, Martin

    2014-01-01

    Video encoding for multimedia services over communication networks has significantly advanced in recent years with the development of the highly efficient and flexible H.264/AVC video coding standard and its SVC extension. The emerging H.265/HEVC video coding standard as well as 3D video coding further advance video coding for multimedia communications. This paper first gives an overview of these new video coding standards and then examines their implications for multimedia communications by studying the traffic characteristics of long videos encoded with the new coding standards. We review video coding advances from MPEG-2 and MPEG-4 Part 2 to H.264/AVC and its SVC and MVC extensions as well as H.265/HEVC. For single-layer (nonscalable) video, we compare H.265/HEVC and H.264/AVC in terms of video traffic and statistical multiplexing characteristics. Our study is the first to examine the H.265/HEVC traffic variability for long videos. We also illustrate the video traffic characteristics and statistical multiplexing of scalable video encoded with the SVC extension of H.264/AVC as well as 3D video encoded with the MVC extension of H.264/AVC.

  19. Video Traffic Characteristics of Modern Encoding Standards: H.264/AVC with SVC and MVC Extensions and H.265/HEVC

    PubMed Central

    2014-01-01

    Video encoding for multimedia services over communication networks has significantly advanced in recent years with the development of the highly efficient and flexible H.264/AVC video coding standard and its SVC extension. The emerging H.265/HEVC video coding standard as well as 3D video coding further advance video coding for multimedia communications. This paper first gives an overview of these new video coding standards and then examines their implications for multimedia communications by studying the traffic characteristics of long videos encoded with the new coding standards. We review video coding advances from MPEG-2 and MPEG-4 Part 2 to H.264/AVC and its SVC and MVC extensions as well as H.265/HEVC. For single-layer (nonscalable) video, we compare H.265/HEVC and H.264/AVC in terms of video traffic and statistical multiplexing characteristics. Our study is the first to examine the H.265/HEVC traffic variability for long videos. We also illustrate the video traffic characteristics and statistical multiplexing of scalable video encoded with the SVC extension of H.264/AVC as well as 3D video encoded with the MVC extension of H.264/AVC. PMID:24701145

  20. [Establishment of RAW264.7 cell strain stably expressing RFP-GFP-LC3].

    PubMed

    Wang, Wan; Zhang, Qing; Zhao, Runpeng; Xu, Xuewei; Xing, Yingru; Zhang, Rongbo; Wu, Jing; Hu, Dong

    2015-09-01

    To establish murine macrophage RAW264.7 cell strain with stable expression of red fluorescent protein-green fluorescent protein-microtubule associated protein light chain 3 (RFP-GFP-LC3). A lentiviral vector containing RFP-GFP-LC3 gene was constructed and then packaged in HEK293T cells with the packaging plasmids. The viral supernatant was collected to infect RAW264.7 cells. The RAW264.7 cell strain with stable expression of RFP-GFP-LC3 was screened with puromycin and analyzed with flow cytometry and fluorescent microscopy for infection efficiency. The number of RFP-GFP-LC3 puncta was observed using florescence microscopy following starvation treatment. The recombinant lentivirus pLV-CMV-RFP-GFP-LC3 was successfully constructed. The RAW264.7 cells with stable expression of RFP-GFP-LC3 were obtained by viral infection and puromycin screening. Fluorescent microscopy and flow cytometry demonstrated the expression rates of RFP and GFP reached to 100%. The number of autophagic puncta significantly increased after starvation treatment. The RAW264.7 cell strain with stable expression of RFP-GFP-LC3 has been successfully constructed, which provides a reliable cellular platform for autophagy research.

  1. Chemoattractant signaling between tumor cells and macrophages regulates cancer cell migration, metastasis and neovascularization.

    PubMed

    Green, Chad E; Liu, Tiffany; Montel, Valerie; Hsiao, Gene; Lester, Robin D; Subramaniam, Shankar; Gonias, Steven L; Klemke, Richard L

    2009-08-21

    Tumor-associated macrophages are known to influence cancer progression by modulation of immune function, angiogenesis, and cell metastasis, however, little is known about the chemokine signaling networks that regulate this process. Utilizing CT26 colon cancer cells and RAW 264.7 macrophages as a model cellular system, we demonstrate that treatment of CT26 cells with RAW 264.7 conditioned medium induces cell migration, invasion and metastasis. Inflammatory gene microarray analysis indicated CT26-stimulated RAW 264.7 macrophages upregulate SDF-1alpha and VEGF, and that these cytokines contribute to CT26 migration in vitro. RAW 264.7 macrophages also showed a robust chemotactic response towards CT26-derived chemokines. In particular, microarray analysis and functional testing revealed CSF-1 as the major chemoattractant for RAW 264.7 macrophages. Interestingly, in the chick CAM model of cancer progression, RAW 264.7 macrophages localized specifically to the tumor periphery where they were found to increase CT26 tumor growth, microvascular density, vascular disruption, and lung metastasis, suggesting these cells home to actively invading areas of the tumor, but not the hypoxic core of the tumor mass. In support of these findings, hypoxic conditions down regulated CSF-1 production in several tumor cell lines and decreased RAW 264.7 macrophage migration in vitro. Together our findings suggest a model where normoxic tumor cells release CSF-1 to recruit macrophages to the tumor periphery where they secrete motility and angiogenic factors that facilitate tumor cell invasion and metastasis.

  2. 40 CFR 264.231 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.231 Section 264.231 Protection of Environment... chemical characteristics of the wastes, including their potential to migrate through soil or to volatilize...

  3. 40 CFR 270.315 - What air emissions control information must I keep at my facility?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... specifications listed in 40 CFR 264.1084(e)(1) or (f)(1). (b) Identification of each container area subject to... or containers under requirements of 40 CFR 264.1084(d)(5) or 264.1086(e)(1)(ii). You must include... monitoring plan for both Method 21 in 40 CFR part 60, appendix A and control device monitoring methods. This...

  4. 40 CFR 270.315 - What air emissions control information must I keep at my facility?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... specifications listed in 40 CFR 264.1084(e)(1) or (f)(1). (b) Identification of each container area subject to... or containers under requirements of 40 CFR 264.1084(d)(5) or 264.1086(e)(1)(ii). You must include... monitoring plan for both Method 21 in 40 CFR part 60, appendix A and control device monitoring methods. This...

  5. 40 CFR 270.315 - What air emissions control information must I keep at my facility?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... specifications listed in 40 CFR 264.1084(e)(1) or (f)(1). (b) Identification of each container area subject to... or containers under requirements of 40 CFR 264.1084(d)(5) or 264.1086(e)(1)(ii). You must include... monitoring plan for both Method 21 in 40 CFR part 60, appendix A and control device monitoring methods. This...

  6. 40 CFR 270.315 - What air emissions control information must I keep at my facility?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... specifications listed in 40 CFR 264.1084(e)(1) or (f)(1). (b) Identification of each container area subject to... or containers under requirements of 40 CFR 264.1084(d)(5) or 264.1086(e)(1)(ii). You must include... monitoring plan for both Method 21 in 40 CFR part 60, appendix A and control device monitoring methods. This...

  7. 40 CFR 270.315 - What air emissions control information must I keep at my facility?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... specifications listed in 40 CFR 264.1084(e)(1) or (f)(1). (b) Identification of each container area subject to... or containers under requirements of 40 CFR 264.1084(d)(5) or 264.1086(e)(1)(ii). You must include... monitoring plan for both Method 21 in 40 CFR part 60, appendix A and control device monitoring methods. This...

  8. Robust Transmission of H.264/AVC Streams Using Adaptive Group Slicing and Unequal Error Protection

    NASA Astrophysics Data System (ADS)

    Thomos, Nikolaos; Argyropoulos, Savvas; Boulgouris, Nikolaos V.; Strintzis, Michael G.

    2006-12-01

    We present a novel scheme for the transmission of H.264/AVC video streams over lossy packet networks. The proposed scheme exploits the error-resilient features of H.264/AVC codec and employs Reed-Solomon codes to protect effectively the streams. A novel technique for adaptive classification of macroblocks into three slice groups is also proposed. The optimal classification of macroblocks and the optimal channel rate allocation are achieved by iterating two interdependent steps. Dynamic programming techniques are used for the channel rate allocation process in order to reduce complexity. Simulations clearly demonstrate the superiority of the proposed method over other recent algorithms for transmission of H.264/AVC streams.

  9. Antipeptide antibodies that can distinguish specific subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.)

    NASA Technical Reports Server (NTRS)

    Cai, X.; Henry, R. L.; Takemoto, L. J.; Guikema, J. A.; Wong, P. P.; Spooner, B. S. (Principal Investigator)

    1992-01-01

    The amino acid sequences of the beta and gamma subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.) root nodules are very similar. However, there are small regions within the sequences that are significantly different between the two polypeptides. The sequences between amino acids 2 and 9 and between 264 and 274 are examples. Three peptides (gamma 2-9, gamma 264-274, and beta 264-274) corresponding to these sequences were synthesized. Antibodies against these peptides were raised in rabbits and purified with corresponding peptide-Sepharose affinity chromatography. Western blot analysis of polyacrylamide gel electrophoresis of bean nodule proteins demonstrated that the anti-beta 264-274 antibodies reacted specifically with the beta polypeptide and the anti-gamma 264-274 and anti-gamma 2-9 antibodies reacted specifically with the gamma polypeptide of the native and denatured glutamine synthetase. These results showed the feasibility of using synthetic peptides in developing antibodies that are capable of distinguishing proteins with similar primary structures.

  10. 40 CFR 264.283 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.283 Section 264.283 Protection of Environment... characteristics of the wastes, including their potential to migrate through soil or to volatilize or escape into...

  11. 40 CFR 264.317 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.317 Section 264.317 Protection of Environment..., including their potential to migrate through the soil or to volatilize or escape into the atmosphere; (2...

  12. 40 CFR 264.259 - Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Special requirements for hazardous wastes FO20, FO21, FO22, FO23, FO26, and FO27. 264.259 Section 264.259 Protection of Environment... through soil or to volatilize or escape into the atmosphere; (2) The attenuative properties of underlying...

  13. Evaluation of H.264 and H.265 full motion video encoding for small UAS platforms

    NASA Astrophysics Data System (ADS)

    McGuinness, Christopher D.; Walker, David; Taylor, Clark; Hill, Kerry; Hoffman, Marc

    2016-05-01

    Of all the steps in the image acquisition and formation pipeline, compression is the only process that degrades image quality. A selected compression algorithm succeeds or fails to provide sufficient quality at the requested compression rate depending on how well the algorithm is suited to the input data. Applying an algorithm designed for one type of data to a different type often results in poor compression performance. This is mostly the case when comparing the performance of H.264, designed for standard definition data, to HEVC (High Efficiency Video Coding), which the Joint Collaborative Team on Video Coding (JCT-VC) designed for high-definition data. This study focuses on evaluating how HEVC compares to H.264 when compressing data from small UAS platforms. To compare the standards directly, we assess two open-source traditional software solutions: x264 and x265. These software-only comparisons allow us to establish a baseline of how much improvement can generally be expected of HEVC over H.264. Then, specific solutions leveraging different types of hardware are selected to understand the limitations of commercial-off-the-shelf (COTS) options. Algorithmically, regardless of the implementation, HEVC is found to provide similar quality video as H.264 at 40% lower data rates for video resolutions greater than 1280x720, roughly 1 Megapixel (MPx). For resolutions less than 1MPx, H.264 is an adequate solution though a small (roughly 20%) compression boost is earned by employing HEVC. New low cost, size, weight, and power (CSWAP) HEVC implementations are being developed and will be ideal for small UAS systems.

  14. Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.

    PubMed

    Wen, Zheng-Shun; Liu, Li-Jia; OuYang, Xiao-Kun; Qu, You-Le; Chen, Yin; Ding, Guo-Fang

    2014-07-01

    This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells. Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide. After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05). SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05). Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05). These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. CLONING AND CHARACTERIZATION OF OSTEOCLAST PRECURSORS FROM THE RAW264.7 CELL LINE

    PubMed Central

    Cuetara, Bethany L. V.; Crotti, Tania N.; O'Donoghue, Anthony J.

    2006-01-01

    SUMMARY Osteoclasts are bone-resorbing cells that differentiate from macrophage precursors in response to receptor activator of NF-κB (RANKL). In vitro models of osteoclast differentiation are principally based on primary cell culture, which are poorly suited to molecular and transgene studies due to the limitations associated with the use of primary macrophage. RAW264.7 is a transfectable macrophage cell line with the capacity to form osteoclast-like cells. In the present study we have identified osteoclast precursors among clones of RAW264.7 cells. RAW264.7 cell were cloned by limiting dilution and induced to osteoclast differentiation by treatment with recombinant RANKL. Individual RAW264.7 cell clones formed tartrate resistant acid phosphatase (TRAP) positive multinuclear cells to various degrees with RANKL treatment. All clones tested expressed the RANKL receptor RANK. Each of the clones expressed the osteoclast marker genes TRAP and cathepsin-K mRNA with RANKL treatment. However, we noted that only select clones were able to form large, well-spread, TRAP positive multinuclear cells. Clones capable of forming large TRAP positive multinuclear cells also expressed β3 integrin and calcitonin receptor mRNAs and were capable of resorbing a mineralized matrix. All clones tested activated NF-κB with RANKL treatment. cDNA expression profiling of osteoclast precursor RAW264.7 cell clones demonstrates appropriate expression of a large number of genes before and after osteoclastic differentiation. These osteoclast precursor RAW264.7 cell clones provide a valuable model for dissecting the cellular and molecular regulation of osteoclast differentiation and activation. PMID:16948499

  16. Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the intracellular bacterial pathogen salmonella within the RAW 264.7 macrophage.

    PubMed

    Kim, Gon Sup; Kim, Dong Hyeok; Lim, Jeong Ju; Lee, Jin Ju; Han, Dae Yong; Lee, Whi Min; Jung, Won Chul; Min, Won Gi; Won, Chung Gil; Rhee, Man Hee; Lee, Hu Jang; Kim, Suk

    2008-11-01

    Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans. Recently, the emergence of multidrug-resistant strains of Salmonella sp. has caused more serious problems in public health. The present study investigated the antibacterial effects of Houttuynia cordata water extract (HCWE) against murine salmonellosis. In RAW 264.7 cells, there was no detectable cytotoxic effect of HCWE at any concentration between 25 and 100 microg/ml after 8-h incubation. The antibacterial activity of HCWE was then examined in a Salmonella enterica serovar (Salmonella typhimurium), and was found to increase in a dose-dependent manner at concentrations from 25 to 100 microg/ml during 8-h incubation. HCWE also affected RAW 264.7 cells including morphologic change and bacterial uptake, but there was no significant difference in bacterial replication in RAW 264.7 cells. With HCWE alone, nitric oxide (NO) production by RAW 264.7 cells did not increase, but when RAW 264.7 cells were infected by S. typhimurium, with or without HCWE, NO production with HCWE was 2-fold higher than that without HCWE. Treatment with HCWE did not affect inducible NO synthase (iNOS) mRNA expression by RAW 264.7 cells, but when RAW 264.7 cells with HCWE were infected by S. typhimurium, iNOS mRNA expression was increased during 8-h incubation. Furthermore, HCWE showed virulence reduction effects in S. typhimurium-infected BALB/c mice. After a lethal dose of S. typhimurium, the mortality rate in the HCWE untreated group was 100% at 7 d, but the HCWE 25, 50, and 100 microg/ml groups survived until 11, 17, and 23 d, respectively. These data suggest that HCWE is stable and beneficial in the treatment of bacterial infection including intracellularly replicating pathogens and may solve antimicrobial misuse and overuse.

  17. 45 CFR 264.50 - What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal to the reduction to the adjusted SFAG resulting from a penalty? 264.50 Section 264.50 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATIO...

  18. 45 CFR 264.50 - What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal to the reduction to the adjusted SFAG resulting from a penalty? 264.50 Section 264.50 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS),...

  19. 45 CFR 264.50 - What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal to the reduction to the adjusted SFAG resulting from a penalty? 264.50 Section 264.50 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS),...

  20. 45 CFR 264.50 - What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal to the reduction to the adjusted SFAG resulting from a penalty? 264.50 Section 264.50 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATIO...

  1. 45 CFR 264.50 - What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false What happens if, in a fiscal year, a State does not expend, with its own funds, an amount equal to the reduction to the adjusted SFAG resulting from a penalty? 264.50 Section 264.50 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS),...

  2. Video Compression Study: h.265 vs h.264

    NASA Technical Reports Server (NTRS)

    Pryor, Jonathan

    2016-01-01

    H.265 video compression (also known as High Efficiency Video Encoding (HEVC)) promises to provide double the video quality at half the bandwidth, or the same quality at half the bandwidth of h.264 video compression [1]. This study uses a Tektronix PQA500 to determine the video quality gains by using h.265 encoding. This study also compares two video encoders to see how different implementations of h.264 and h.265 impact video quality at various bandwidths.

  3. Comparative assessment of H.265/MPEG-HEVC, VP9, and H.264/MPEG-AVC encoders for low-delay video applications

    NASA Astrophysics Data System (ADS)

    Grois, Dan; Marpe, Detlev; Nguyen, Tung; Hadar, Ofer

    2014-09-01

    The popularity of low-delay video applications dramatically increased over the last years due to a rising demand for realtime video content (such as video conferencing or video surveillance), and also due to the increasing availability of relatively inexpensive heterogeneous devices (such as smartphones and tablets). To this end, this work presents a comparative assessment of the two latest video coding standards: H.265/MPEG-HEVC (High-Efficiency Video Coding), H.264/MPEG-AVC (Advanced Video Coding), and also of the VP9 proprietary video coding scheme. For evaluating H.264/MPEG-AVC, an open-source x264 encoder was selected, which has a multi-pass encoding mode, similarly to VP9. According to experimental results, which were obtained by using similar low-delay configurations for all three examined representative encoders, it was observed that H.265/MPEG-HEVC provides significant average bit-rate savings of 32.5%, and 40.8%, relative to VP9 and x264 for the 1-pass encoding, and average bit-rate savings of 32.6%, and 42.2% for the 2-pass encoding, respectively. On the other hand, compared to the x264 encoder, typical low-delay encoding times of the VP9 encoder, are about 2,000 times higher for the 1-pass encoding, and are about 400 times higher for the 2-pass encoding.

  4. Immunomodulatory effects of xanthan gum in LPS-stimulated RAW 264.7 macrophages.

    PubMed

    Liu, Fuyan; Zhang, Xiaofeng; Ling, Peixue; Liao, Joshua; Zhao, Mingsheng; Mei, Li; Shao, Huarong; Jiang, Peng; Song, Zhigang; Chen, Qixin; Wang, Fengshan

    2017-08-01

    In this study, we evaluated the immunomodulatory effects of xanthan gum (XG) in RAW264.7 macrophages and the underlying molecular mechanisms. We used scanning electron microscopy (SEM) to analyze the morphology of XG-treated RAW264.7 cells with and without lipopolysaccharide (LPS) stimulation and investigated the subsequent effects on nitric oxide (NO), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor (TNF-α), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) levels in LPS-activated mouse RAW264.7 macrophages. We also analyzed the binding affinity of XG to Toll-like receptor 4 (TLR4) with surface plasmon resonance (SPR) analysis and observed that XG decreased NO, IL-6 and TNF-α secretion into the culture medium and iNOS and COX-2 protein levels induced by LPS. This study reveals a two-way immunomodulatory effect of XG on inflammatory mediators in RAW264.7 macrophages that may involve the TLR4 signal pathway, providing a pharmacological basis for the use of XG in the control of inflammatory disorders. Copyright © 2017. Published by Elsevier Ltd.

  5. 36 CFR 264.10 - Establishment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MANAGEMENT Mount St. Helens National Volcanic Monument Symbol § 264.10 Establishment. There is hereby established an official symbol, as depicted herein, to designate and represent the Mount St. Helens National...

  6. 6-Gingerol Suppresses Adipocyte-Derived Mediators of Inflammation In Vitro and in High-Fat Diet-Induced Obese Zebra Fish.

    PubMed

    Choi, Jia; Kim, Kui-Jin; Kim, Byung-Hak; Koh, Eun-Jeong; Seo, Min-Jung; Lee, Boo-Yong

    2017-02-01

    The present study was performed to investigate the molecular mechanism of 6-gingerol on adipocyte-mediated systemic inflammation in vitro and in high-fat diet-induced obese zebra fish. 6-Gingerol decreased adipogenesis due to the suppression of adipocyte differentiation markers, including peroxisome proliferator-activated receptor gamma, CCAATT enhancer binding protein α , and adipocyte protein 2, and triglyceride synthesis enzymes, including sterol regulatory element-binding protein-1, fatty acid synthase, lysophosphatidic acid acyltransferase, and acyl-coA : diacylglycerol acyltransferase 1, in 3T3-L1. A coculture insert system using 3T3-L1 with RAW 264.7 (coculture insert system using fully differentiated 3T3-L1 cells with RAW 264.7 macrophages) revealed that 6-gingerol increased anti-inflammatory cytokine interleukin-10. The expression of TNF α , monocyte chemotactic protein-1, interleukin-1 β , and interleukin-6 were decreased in the coculture insert system using fully differentiated 3T3-L1 cells with RAW 264.7 macrophages treated with 6-gingerol. Moreover, the coculture insert system using fully differentiated 3T3-L1 cells with RAW 264.7 macrophages treated with 6-gingerol inhibited the protein expression of TNF α and monocyte chemotactic protein-1 in RAW 264.7. 6-Gingerol decreased c-JUN N-terminal kinase and I kappa B kinase beta and its downstream target AP-1 expression in the coculture insert system using fully differentiated 3T3-L1 cells with RAW 264.7 macrophages. Furthermore, 6-gingerol decreased the expression of inducible nitric oxide synthase stimulated by the coculture insert system using fully differentiated 3T3-L1 cells with RAW 264.7 macrophages in RAW 264.7 and attenuated nitric oxide production in diet-induced obese zebra fish. Our results suggest that 6-gingerol suppresses inflammation through the regulation of the c-JUN N-terminal kinase-I kappa B kinase beta and its downstream targets. Georg Thieme Verlag KG Stuttgart · New York.

  7. 36 CFR 264.13 - Unauthorized use.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MANAGEMENT Mount St. Helens National Volcanic Monument Symbol § 264.13 Unauthorized use. Except as provided... St. Helens National Volcanic Monument may be subject to criminal penalty under 18 U.S.C. 701. ...

  8. 75 FR 26227 - Product Cancellation Order for Certain Pesticide Registrations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-11

    ... Butoxide MGK 264 000769-00620 SMCP Malathion 57% Malathion Premium Grade 000769-00621 SMCP Malathion EM-5... Home & Garden Pyrethrins Insect Bomb Piperonyl Butoxide MGK 264 000769-00857 Science Red Arrow...

  9. 40 CFR 264.250 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... so that neither run-off nor leachate is generated is not subject to regulation under § 264.251 or..., by means other than wetting; and (4) The pile will not generate leachate through decomposition or...

  10. 40 CFR 264.250 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... so that neither run-off nor leachate is generated is not subject to regulation under § 264.251 or..., by means other than wetting; and (4) The pile will not generate leachate through decomposition or...

  11. 40 CFR 264.250 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... so that neither run-off nor leachate is generated is not subject to regulation under § 264.251 or..., by means other than wetting; and (4) The pile will not generate leachate through decomposition or...

  12. 40 CFR 264.250 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... so that neither run-off nor leachate is generated is not subject to regulation under § 264.251 or..., by means other than wetting; and (4) The pile will not generate leachate through decomposition or...

  13. 40 CFR 264.250 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... so that neither run-off nor leachate is generated is not subject to regulation under § 264.251 or..., by means other than wetting; and (4) The pile will not generate leachate through decomposition or...

  14. 76 FR 4686 - Product Cancellation Order for Certain Pesticide Registrations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ... Duty Bird Paradichlorobenze Guard. ne. 002517-00049 Sergeant's Pump Soap Bioallethrin MGK for Dogs. 264 Phenothrin. 002517-00059 Sergeant's Skip-Flea MGK 264 Soap (with D- Phenothrin. Phenothrin). 002517-00067...

  15. Studies on palauan medicinal herbs. II. Activation of mouse macrophages RAW 264.7 by Ongael, leaves of Phaleria cumingii (Meisn.) F. Vill. and its acylglucosylsterols.

    PubMed

    Matsuda, Hideaki; Tokunaga, Masashi; Iwahashi, Hiroyasu; Naruto, Shunsuke; Yagi, Hideki; Masuko, Takashi; Kubo, Michinori

    2005-05-01

    The extract of Ongael [leaves of Phaleria cumingii (MEISN.) F. VILL.], a Palauan medicinal herb, enhanced an in vitro phagocytic activity of mouse macrophages RAW 264.7 cells (RAW 264.7). Activity-guided fractionation of the Ongael extract by the in vitro phagocytosis assay using RAW 264.7 led to the isolation of a mixture of acylglucosylsterols (1) as an active constituent along with other inactive constituents, tetracosanol and mangiferin. On the basis of chemical modifications and spectral analyses, the compound 1 was deduced to be a mixture of the known 3-O-(6-O-acyl-beta-D-glucosyl)-beta-sitosterols, the acyl moiety being mainly palmitoyl (57%), oleoyl (12%) and alpha-linolenoyl (12%) with small amount of stearoyl (7%) and linoleoyl (4%).

  16. [Effect of qinghuobaiduyin on the expression of high mobility group box chromosomal protein 1 in macrophage].

    PubMed

    Li, Ping; Xu, Dan; Luo, Chengqun

    2010-07-01

    To observe the expression of high mobility group box chromosomal protein 1(HMGB1) in RAW264.7 macrophages after interfering with burning serum and qinghuobaidu-yin (QHBDY), and to find out the endogenous protection mechanism of QHBDY resisting inflammation reaction. RT-PCR was used to detect the expression of HMGB1 in RAW264.7 macrophages after interfering RAW264.7 macrophages with normal SD rat serum, burning SD rat serum, and QHBDY feeding SD rat serum. Small quantity of HMGB1 mRNA was expressed in RAW264.7. The expression of HMGB1 mRNA fluctuated around the standard level after interfering with normal serum of SD rats. The expression of HMGB1 mRNA rose at 3 h, and then decreased to the standard level; at 18 h, it rose rapidly; at 36 h, it reached the peak; and at 48 h, it remained at the high level after interfering with burning serum. The expression of HMGB1 mRNA increased at 3 h, and then decreased to the standard level. At 24 h, it started to rise after interfering with herb serum, and was lower than that of; the burning serum group (P<0.05). Burning serum can increase the expression of HMGB1 mRNA in RAW264.7. QHBDY can decrease the high expression of HMGB1 mRNA in RAW264.7 caused by burning serum.

  17. [Gallic acid inhibits inflammatory response of RAW264.7 macrophages by blocking the activation of TLR4/NF-κB induced by LPS].

    PubMed

    Huang, Lihua; Hou, Lin; Xue, Hainan; Wang, Chunjie

    2016-12-01

    Objective To observe the influence of gallic acid on Toll-like receptor 4/nuclear factor-κB (TLR4/NF-κB) pathway in the RAW264.7 macrophages stimulated by lipopolysaccharide (LPS). Methods RAW264.7 macrophages were divided into the following groups: control group, LPS group, LPS combined with gallic acid group, LPS combined with pyrrolidine dithiocarbamate (PDTC) group and LPS combined with dexamethasone (DM) group. RAW264.7 cells were cultured for 24 hours after corresponding treatments. The levels of tumor necrosis factor α (TNF-α), interleukin-1 (IL-1) and IL-6 were detected by ELISA. The levels of TLR4 and NF-κB mRNAs were tested by real-time PCR. The levels of p-IκBα, p65, p-p65 and TLR4 proteins were examined by Western blotting. Results The expression levels of TNF-α, IL-1 and IL-6 were up-regulated in the RAW264.7 macrophages after stimulated by LPS. Gallic acid could reduce the elevated expression levels of TNF-α, IL-1 and IL-6 induced by LPS. The expression of TLR4 significantly increased after stimulated by LPS and NF-κB was activated. Gallic acid could reverse the above changes and prevent the activation of NF-κB. Conclusion Gallic acid could inhibit LPS-induced inflammatory response in RAW264.7 macrophages via TLR4/NF-κB pathway.

  18. Targeting RAW 264.7 macrophages (M1 type) with Withaferin-A decorated mannosylated liposomes induces repolarization via downregulation of NF-κB and controlled elevation of STAT-3.

    PubMed

    Neog, Manoj Kumar; Sultana, Farhath; Rasool, Mahaboobkhan

    2018-05-25

    In the present study, we intend to gain an insight into the mechanism of Withaferin-A (WA), a steroidal lactone with reference to repolarization of RAW 264.7 macrophages (M1 to M2 type). We found that successful internalization of WA via mannosylated liposomal delivery system (ML-WA) reduced the RAW 264.7 macrophage (M1) mediated pro-inflammatory cytokines (IL-1β, IL-6, IL-23, and TNF-α) through the attenuation of transcription factor NF-κB-p65 expression. Whereas, ML-WA treatment induced a controlled upregulation of p-STAT3, and ablated the key oxidative stress markers (NO, iNOS, and ROS) in M1 → M2 RAW 264.7 macrophage repolarization, which suggested the recalibration of M1 macrophage metabolic function. Further, the elevated expression of M2 macrophage associated CD163 over the M1 macrophage related CD86 concluded that ML-WA induces an anti-inflammatory response by repolarizing the M1 → M2 RAW 264.7 macrophage. Copyright © 2018 Elsevier B.V. All rights reserved.

  19. 40 CFR 264.32 - Required equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Preparedness and Prevention § 264.32 Required equipment. All facilities must be equipped with the following... referred to above must do so with part B of the permit application.] [45 FR 33221, May 19, 1980, as amended...

  20. 40 CFR 264.1202 - Closure and post-closure care.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1202 Closure and post-closure care. (a) At... it remains in service as a munitions or explosives magazine or storage unit. (b) If, after removing...

  1. 40 CFR 264.1202 - Closure and post-closure care.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1202 Closure and post-closure care. (a) At... it remains in service as a munitions or explosives magazine or storage unit. (b) If, after removing...

  2. 40 CFR 264.1202 - Closure and post-closure care.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1202 Closure and post-closure care. (a) At... it remains in service as a munitions or explosives magazine or storage unit. (b) If, after removing...

  3. 40 CFR 264.1202 - Closure and post-closure care.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... FACILITIES Hazardous Waste Munitions and Explosives Storage § 264.1202 Closure and post-closure care. (a) At... it remains in service as a munitions or explosives magazine or storage unit. (b) If, after removing...

  4. RCRA, superfund and EPCRA hotline training module. Introduction to: Miscellaneous and other units (40 cfr part 264, subpart x and 40 cfr part 265, subparts p, q, and r) updated July 1996

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-07-01

    The module describes the basic requirements and types of units of 40 CFR Part 264, Subpart X and standards for broadly defined treatment processes - Thermal treatment (Part 265, Subpart P); chemical, physical, and biological treatment (Subpart Q); and underground injection (Subpart R). Explains when corrective action applies to these subparts. It presents the relationship between Part 264, Subpart X, and Part 265, Subparts P, Q, and R.

  5. Anti-inflammatory effects of ursodeoxycholic acid by lipopolysaccharide-stimulated inflammatory responses in RAW 264.7 macrophages

    PubMed Central

    Ko, Wan-Kyu; Lee, Soo-Hong; Kim, Sung Jun; Jo, Min-Jae; Kumar, Hemant; Han, In-Bo; Sohn, Seil

    2017-01-01

    Purpose The aim of this study was to investigate the anti-inflammatory effects of Ursodeoxycholic acid (UDCA) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods We induced an inflammatory process in RAW 264.7 macrophages using LPS. The anti-inflammatory effects of UDCA on LPS-stimulated RAW 264.7 macrophages were analyzed using nitric oxide (NO). Pro-inflammatory and anti-inflammatory cytokines were analyzed by quantitative real time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylations of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 in mitogen-activated protein kinase (MAPK) signaling pathways and nuclear factor kappa-light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) signaling pathways were evaluated by western blot assays. Results UDCA decreased the LPS-stimulated release of the inflammatory mediator NO. UDCA also decreased the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin 1-α (IL-1α), interleukin 1-β (IL-1β), and interleukin 6 (IL-6) in mRNA and protein levels. In addition, UDCA increased an anti-inflammatory cytokine interleukin 10 (IL-10) in the LPS-stimulated RAW 264.7 macrophages. UDCA inhibited the expression of inflammatory transcription factor nuclear factor kappa B (NF-κB) in LPS-stimulated RAW 264.7 macrophages. Furthermore, UDCA suppressed the phosphorylation of ERK, JNK, and p38 signals related to inflammatory pathways. In addition, the phosphorylation of IκBα, the inhibitor of NF-κB, also inhibited by UDCA. Conclusion UDCA inhibits the pro-inflammatory responses by LPS in RAW 264.7 macrophages. UDCA also suppresses the phosphorylation by LPS on ERK, JNK, and p38 in MAPKs and NF-κB pathway. These results suggest that UDCA can serve as a useful anti-inflammatory drug. PMID:28665991

  6. Saccharum Alhagi polysaccharide-1 and −2 promote the immunocompetence of RAW264.7 macrophages in vitro

    PubMed Central

    Li, Gairu; Xiang, Yang; Zhao, Jin; Chang, Junmin

    2018-01-01

    The in vitro immune activities of Saccharum Alhagi polysaccharides (SAP) have been previously studied. The present study aimed to investigate the effects of SAP-1 and SAP-2 on the activity of RAW264.7 mouse macrophages. RAW264.7 cells were treated with 150, 300 and 600 mg/l concentrations of SAP-1 (a 50% alcohol precipitation) and SAP-2 (an 80% alcohol precipitation) or with 10 mg/l lipopolysaccharide. Untreated cells were used as a negative control. An MTT assay was used to detect the proliferation of the cells, and Hoechst 33528 staining was conducted in order to visualize the cell nuclei. Additionally, the Griess method was used to measure nitric oxide (NO) levels. A neutral red uptake assay was performed to determine the phagocytic activity of the macrophages, and ELISAs were performed to detect cytokine levels. Furthermore, reverse transcription-quantitative polymerase chain reaction was used to measure the mRNA expression of certain cytokines. The results demonstrated that SAP increased the proliferative activity and activated the immune function of RAW264.7 cells, and was lacking in cytotoxicity. In addition, SAP-1 exhibited a stronger effect in promoting RAW264.7 cell proliferation than did SAP-2. Furthermore, SAP-1 and SAP-2 significantly increased the level of NO, with the effect of SAP-1 being stronger than that of SAP-2. SAP-1 increased the phagocytic activity of RAW264.7 cells and promoted the secretion of the cytokines interleukin (IL)-1β, IL-2 and tumor necrosis factor (TNF)-α by RAW264.7 cells, with an effect that was stronger than that of SAP-2. Finally, different concentrations of SAP-1 or SAP-2 had distinct effects in upregulating the expression of TNF-α, IL-1β, nuclear factor-κB and inducible nitric oxide synthase mRNA. The results of the present study demonstrate that SAP is capable of enhancing the immune activity of mouse macrophages. PMID:29545883

  7. The Anti-Inflammatory Effects of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Higher Basidiomycetes) in a Coculture System of 3T3-L1 Adipocytes and RAW264 Macrophages.

    PubMed

    Mori, Koichiro; Ouchi, Kenji; Hirasawa, Noriyasu

    2015-01-01

    Chronic low-grade inflammation in the adipose tissue accompanying obesity is thought to be an underlying driver of metabolic diseases. In this study, we aimed to investigate the efficacy of Hericium erinaceus on adipose tissue inflammation. The anti-inflammatory effects of the ethyl acetate soluble fraction of H. erinaceus (EAHE) were examined using cocultures of 3T3-L1 adipocytes and RAW264 macrophages. EAHE significantly suppressed tumor necrosis factor (TNF)-α and interleukin (IL)-6 production in cultured RAW264 macrophages stimulated by lipopolysaccharide (LPS). EAHE also caused notable inhibition of c-Jun N-terminal kinase (JNK) activation, which is thought to be involved in the suppression of proinflammatory cytokines by EAHE. In a coculture system with 3T3-L1 and RAW264 cells stimulated with LPS, EAHE reduced TNF-α and IL-6 concentrations in the conditioned medium and lowered the gene expression levels of these cytokines in 3T3-L1 adipocytes. Furthermore, EAHE suppressed the LPS-induced reduction of adiponectin mRNA levels in 3T3-L1 adipocytes cocultured with RAW264 macrophages. However, in 3T3-L1 adipocytes cultured alone, the concentration of LPS used in this study did not affect the gene expression levels of these adipokines. We attributed the anti-inflammatory effects of EAHE on 3T3-L1 adipocytes cocultured with RAW264 macrophages to the suppression of Toll-like receptor 4 (TLR4) signaling and subsequent proinflammatory cytokine secretion in RAW264 cells. Our findings indicate the possibility that H. erinaceus exerts anti-inflammatory effects on macrophages through the inhibition of TLR4-JNK signaling and prevents or ameliorates adipose tissue inflammation associated with obesity.

  8. 24 CFR 203.264 - Payment of periodic MIP.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Payment § 203.264 Payment of periodic MIP. The mortgagee shall pay each MIP in twelve equal monthly installments. Each monthly installment shall be due and payable to the Commissioner no later than the tenth day...

  9. 41 CFR 105-60.303 - Rules for public inspection and copying.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., New York, the Commonwealth of Puerto Rico, and the Virgin Islands) General Services Administration (2AR) 26 Federal Plaza, New York, NY 10278 Telephone: 212-264-1234 FAX: 212-264-2760 Mid-Atlantic...

  10. 40 CFR 264.1055 - Standards: Sampling connection systems.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... that complies with the requirements of § 264.1060 of this subpart. (c) In-situ sampling systems and... collected or captured. (b) Each closed-purge, closed-loop, or closed-vent system as required in paragraph (a...

  11. 40 CFR 264.1055 - Standards: Sampling connection systems.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... that complies with the requirements of § 264.1060 of this subpart. (c) In-situ sampling systems and... collected or captured. (b) Each closed-purge, closed-loop, or closed-vent system as required in paragraph (a...

  12. 40 CFR 264.1055 - Standards: Sampling connection systems.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... that complies with the requirements of § 264.1060 of this subpart. (c) In-situ sampling systems and... collected or captured. (b) Each closed-purge, closed-loop, or closed-vent system as required in paragraph (a...

  13. Suppression of the lipopolysaccharide-induced expression of MARCKS-related protein (MRP) affects transmigration in activated RAW264.7 cells.

    PubMed

    Chun, Kwang-Rok; Bae, Eun Mi; Kim, Jae-Kwan; Suk, Kyoungho; Lee, Won-Ha

    2009-01-01

    The molecular action mechanism of MRP, one of the protein kinase C (PKC) substrates, has been under intense investigation, but reports on its role in macrophage function remain controversial. The treatment of macrophage cell lines with bacterial lipopolysaccharide (LPS) induced a high level of MRP expression suggesting that MRP plays a role in the function of activated macrophages. In order to investigate the role of MRP in activated RAW264.7 cells, we stably transfected MRP-specific shRNA expression constructs and tested for alterations in macrophage-related functions. The down-regulation of MRP expression resulted in a marked reduction in chemotaxis toward MCP-1 or extracellular matrix proteins. Furthermore, pharmacological inhibitors of PKC significantly inhibited the chemotaxis in RAW264.7 cells. These data reveals the pivotal role of MRP in the transmigration of activated RAW264.7 cells.

  14. Platinum Nanoparticles Induce Apoptosis on Raw 264.7 Macrophage Cells.

    PubMed

    Loan, Ta Thi; Do, Le Thanh; Yoo, Hoon

    2018-02-01

    The cellular effects of platinum nanoparticles (PNP05, average size of 5 nm, and PNP30, average size of 30 nm) were investigated on murine leukemia Raw 264.7 cells. Cells treated with various concentrations of PNPs showed size-dependent cytotoxicity in an MTT assay with PNP5 of smaller nanoparticles higher toxicity than PNP30. Investigations on cell morphology, Annexin V assay, DNA fragmentation and the activity of caspase-3/-7 showed that PNPs induced apoptosis on Raw 264.7 cells by changing cell morphology and density, increasing cell population in apoptosis and causing nucleus fragmentation. Further study on caspase activity by Western blotting revealed that the apoptosis was induced by the activation of caspase-3 and -7. In addition, PNPs inactivated DNA repair system, generating dose-dependent DNA ladder bands on agarose gel electrophoresis. Taken together, PNPs triggered cytotoxicity on Raw 264.7 cells by suppressing cell growth/survival and inducing apoptosis.

  15. [Inhibitory effects of pseudolaric acid B on inflammatory response and M1 phenotype polarization in RAW264.7 macrophages induced by lipopolysaccharide].

    PubMed

    Li, Yuxiu; Li, Tan; Ji, Wenjie; Li, Xiao; Ma, Yongqiang; Zhao, Jihong; Zhou, Xin; Li, Yuming

    2016-05-01

    To investigate the effects of pseudolaric acid B (PLAB) on the inflammatory response and M1 phenotype polarization in RAW264.7 cells induced by lipopolysaccharide (LPS) and the related mechanisms. The inflammatory model in vitro was made using RAW264.7 cells stimulated by LPS, and then was treated with 0.5 μmol/L PLAB and 1 μmol/L GW9662, a peroxisome proliferators-activated receptor γ (PPARγ) antagonist. The cell cycle was tested by flow cytometry. The mRNA expressions of PPARγ and M1 phenotype markers interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) were measured by real-time PCR. The expression levels of signal molecules involved in nuclear factor-κB (NF-κB) signal pathway were detected by Western blotting. PLAB markedly decreased the expressions of IL-1β and TNF-α mRNAs induced by LPS and increased PPARγ mRNA level. Moreover, the expressions of NF-κB p65, pNF-κB p65, IKKα, IKKβ, pIKKα/β, IκBα and pIκBα decreased in PLAB-treated cells. Meanwhile, RAW264.7 cells were arrested in G0 and G2 phase after the treatment with PLAB. However, the effects of PLAB on RAW264.7 cells could be reversed by GW9662 obviously. PLAB could inhibit the inflammatory response and M1 phenotype polarization in RAW264.7 cells induced by LPS via modulating cell cycle and NF-κB/PPARγ signal pathway.

  16. Roadway lighting study : Rte. 264 in downtown Norfolk.

    DOT National Transportation Integrated Search

    1974-01-01

    A study of roadway lighting is currently being conducted at several locations in Virginia by using a mobile illumination recording system to obtain lighting measurements. Areas being surveyed include the I-264 interchange area in downtown Norfolk and...

  17. 40 CFR 264.96 - Compliance period.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....96 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Releases From Solid Waste Management Units § 264.96 Compliance period. (a) The Regional Administrator will...

  18. 40 CFR 264.279 - Recordkeeping.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.279 Recordkeeping. The owner or operator must include hazardous waste application dates and rates in...

  19. 40 CFR 264.279 - Recordkeeping.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.279 Recordkeeping. The owner or operator must include hazardous waste application dates and rates in...

  20. 40 CFR 264.279 - Recordkeeping.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.279 Recordkeeping. The owner or operator must include hazardous waste application dates and rates in...

  1. 40 CFR 264.279 - Recordkeeping.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.279 Recordkeeping. The owner or operator must include hazardous waste application dates and rates in...

  2. 40 CFR 264.340 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... finds that the waste will pose a threat to human health and the environment when burned in an... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Incinerators § 264...

  3. 40 CFR 264.111 - Closure performance standard.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 264.111 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... eliminates, to the extent necessary to protect human health and the environment, post-closure escape of...

  4. 40 CFR 264.340 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... finds that the waste will pose a threat to human health and the environment when burned in an... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Incinerators § 264...

  5. 40 CFR 264.340 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... finds that the waste will pose a threat to human health and the environment when burned in an... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Incinerators § 264...

  6. 40 CFR 264.111 - Closure performance standard.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 264.111 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... eliminates, to the extent necessary to protect human health and the environment, post-closure escape of...

  7. 40 CFR 264.93 - Hazardous constituents.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 264.93 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Releases... the ground-water quality; (vii) The potential for health risks caused by human exposure to waste...

  8. 40 CFR 264.93 - Hazardous constituents.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 264.93 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Releases... the ground-water quality; (vii) The potential for health risks caused by human exposure to waste...

  9. 40 CFR 264.72 - Manifest discrepancies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 264.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Manifest... waste acid, or toxic constituents not reported on the manifest or shipping paper. (c) Upon discovering a...

  10. 40 CFR 264.72 - Manifest discrepancies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 264.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Manifest... waste acid, or toxic constituents not reported on the manifest or shipping paper. (c) Upon discovering a...

  11. 40 CFR 264.72 - Manifest discrepancies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 264.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Manifest... waste acid, or toxic constituents not reported on the manifest or shipping paper. (c) Upon discovering a...

  12. 40 CFR 264.72 - Manifest discrepancies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 264.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Manifest... waste acid, or toxic constituents not reported on the manifest or shipping paper. (c) Upon discovering a...

  13. 40 CFR 264.72 - Manifest discrepancies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 264.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Manifest... waste acid, or toxic constituents not reported on the manifest or shipping paper. (c) Upon discovering a...

  14. 40 CFR 264.220 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Surface Impoundments... that use surface impoundments to treat, store, or dispose of hazardous waste except as § 264.1 provides...

  15. 40 CFR 264.54 - Amendment of contingency plan.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 264.54 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... of hazardous waste or hazardous waste constituents, or changes the response necessary in an emergency...

  16. 40 CFR 264.54 - Amendment of contingency plan.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 264.54 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... of hazardous waste or hazardous waste constituents, or changes the response necessary in an emergency...

  17. 78 FR 20642 - Notice of Proposed CERCLA Agreement and Order on Consent for Removal Action by Bona Fide...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-05

    ... Fernando Valley Area 2 Superfund Site, Glendale Chromium Operable Unit, 264 W. Spazier Ave., Burbank... Site, Glendale Chromium Operable Unit, located at 264 W. Spazier Ave., Burbank, California. Under the...

  18. Draft Genome Sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a Citrate-Fermenting Strain

    PubMed Central

    Zuljan, Federico; Espariz, Martín; Blancato, Victor S.; Esteban, Luis; Alarcón, Sergio

    2016-01-01

    We report the draft genome sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a natural strain isolated from artisanal cheese from northwest Argentina. L. lactis subsp. lactis bv. diacetylactis is one of the most important microorganisms used as starter culture around the world. The CRL264 strain constitutes a model microorganism in the studies on the generation of aroma compounds (diacetyl, acetoin, and 2,3-butanediol) by lactic acid bacteria. Our genome analysis shows similar genetic organization to other available genomes of L. lactis bv. diacetylactis strains. PMID:26847906

  19. INHIBITORY EFFECT OF EMODIN ON RAW 264.7 ACTIVATED WITH DOUBLE STRANDED RNA ANALOGUE POLY I:C.

    PubMed

    Kim, Young-Jin; Lee, Ji Young; Kim, Hyun-Ju; Kim, Do-Hoon; Lee, Tae Hee; Kang, Mi Suk; Choi, You-Kyung; Lee, Hye Lim; Kim, Jaieun; An, Hyo-Jin; Park, Wansu

    2017-01-01

    Emodin (3-methyl-1, 6, 8-trihydroxyanthraquinone) is a compound which can be found in Polygoni Multiflori Radix (PMR). PMR is the root of Polygonum multiflorum . PMR is used to treat dizziness, spermatorrhea, sores, and scrofula as well as chronic malaria traditionally in China and Korea. The anti-tumor property of emodin was already reported. However, anti-viral activity of emodin on macrophages are not fully reported. Effects of emodin on RAW 264.7 mouse macrophages induced by polyinosinic-polycytidylic acid (poly I:C), a synthetic analog of double-stranded RNA, were evaluated. Emodin restored the cell viability in poly I: C-induced RAW 264.7 at concentrations of up to 50 μM. Emodin significantly inhibited the production of nitric oxide, IL-1α, IL-Ιβ, IL-6, GM-CSF, G-CSF, M-CSF, MCP-1, MIP-1a, MIP-Ιβ, MIP-2, RANTES, and IP-10 as well as calcium release and mRNA expression of signal transducer and activated transcription 1 (STAT1) in poly I:C-induced RAW 264.7 ( P < 0.05). This study shows the inhibitory effect of emodin on poly I: C-induced RAW 264.7 via calcium-STAT pathway.

  20. INHIBITORY EFFECT OF EMODIN ON RAW 264.7 ACTIVATED WITH DOUBLE STRANDED RNA ANALOGUE POLY I:C

    PubMed Central

    Kim, Young-Jin; Lee, Ji Young; Kim, Hyun-Ju; Kim, Do-Hoon; Lee, Tae Hee; Kang, Mi Suk; Choi, You-Kyung; Lee, Hye Lim; Kim, Jaieun; An, Hyo-Jin; Park, Wansu

    2017-01-01

    Background: Emodin (3-methyl-1, 6, 8-trihydroxyanthraquinone) is a compound which can be found in Polygoni Multiflori Radix (PMR). PMR is the root of Polygonum multiflorum. PMR is used to treat dizziness, spermatorrhea, sores, and scrofula as well as chronic malaria traditionally in China and Korea. The anti-tumor property of emodin was already reported. However, anti-viral activity of emodin on macrophages are not fully reported. Materials and Methods: Effects of emodin on RAW 264.7 mouse macrophages induced by polyinosinic-polycytidylic acid (poly I:C), a synthetic analog of double-stranded RNA, were evaluated. Results: Emodin restored the cell viability in poly I: C-induced RAW 264.7 at concentrations of up to 50 μM. Emodin significantly inhibited the production of nitric oxide, IL-1α, IL-Ιβ, IL-6, GM-CSF, G-CSF, M-CSF, MCP-1, MIP-1a, MIP-Ιβ, MIP-2, RANTES, and IP-10 as well as calcium release and mRNA expression of signal transducer and activated transcription 1 (STAT1) in poly I:C-induced RAW 264.7 (P < 0.05). Conclusion: This study shows the inhibitory effect of emodin on poly I: C-induced RAW 264.7 via calcium-STAT pathway. PMID:28480427

  1. Eicosapentaenoic Acid (EPA) Induced Macrophages Activation through GPR120-Mediated Raf-ERK1/2-IKKβ-NF-κB p65 Signaling Pathways

    PubMed Central

    Han, Lirong; Song, Shumin; Niu, Yabing; Meng, Meng; Wang, Chunling

    2017-01-01

    Objectives: To investigate the immunomodulatory effect and molecular mechanisms of Eicosapentaenoic acid (EPA, a typical kind of n-3PUFAs) on RAW264.7 cells. Methods: A variety of research methods, including the RAW264.7 cells culture, cell proliferation assays, morphologic observations, measurements of NO production, cytokine assays, nuclear protein extractions, western blot analyses and NF-κB p65 immunofluorescence assays were used in this study. Results: The results showed that EPA could increase the proliferation index and enhance the release of nitric oxide (NO) and cytokines in RAW264.7 cells. Western blotting results revealed that the protein level of GPR120 increased significantly in RAW264.7 cells after EPA treatment. Meanwhile, EPA elevated the phosphorylation status of Raf, which may act as an upstream regulator of EPA-induced phosphorylated ERK1/2. In addition, the phosphorylated ERK1/2 may then promote IKKβ in endochylema and translocate the NF-κB p65 subunit into the nucleus, thus regulating the production of inducible nitric oxide synthase (iNOS) and cytokines. Conclusions: EPA (0.6–3.0 μmol) activates RAW264.7 cells through GPR120-mediated Raf-ERK1/2-IKKβ-NF-κB p65 signaling pathways. PMID:28841192

  2. Eicosapentaenoic Acid (EPA) Induced Macrophages Activation through GPR120-Mediated Raf-ERK1/2-IKKβ-NF-κB p65 Signaling Pathways.

    PubMed

    Han, Lirong; Song, Shumin; Niu, Yabing; Meng, Meng; Wang, Chunling

    2017-08-25

    Objectives: To investigate the immunomodulatory effect and molecular mechanisms of Eicosapentaenoic acid (EPA, a typical kind of n-3PUFAs) on RAW264.7 cells. Methods: A variety of research methods, including the RAW264.7 cells culture, cell proliferation assays, morphologic observations, measurements of NO production, cytokine assays, nuclear protein extractions, western blot analyses and NF-κB p65 immunofluorescence assays were used in this study. Results: The results showed that EPA could increase the proliferation index and enhance the release of nitric oxide (NO) and cytokines in RAW264.7 cells. Western blotting results revealed that the protein level of GPR120 increased significantly in RAW264.7 cells after EPA treatment. Meanwhile, EPA elevated the phosphorylation status of Raf, which may act as an upstream regulator of EPA-induced phosphorylated ERK1/2. In addition, the phosphorylated ERK1/2 may then promote IKKβ in endochylema and translocate the NF-κB p65 subunit into the nucleus, thus regulating the production of inducible nitric oxide synthase (iNOS) and cytokines. Conclusions: EPA (0.6-3.0 μmol) activates RAW264.7 cells through GPR120-mediated Raf-ERK1/2-IKKβ-NF-κB p65 signaling pathways.

  3. Crosstalk between autophagy and apoptosis in RAW 264.7 macrophages infected with ectromelia orthopoxvirus.

    PubMed

    Martyniszyn, Lech; Szulc-Dąbrowska, Lidia; Boratyńska-Jasińska, Anna; Struzik, Justyna; Winnicka, Anna; Niemiałtowski, Marek

    2013-10-01

    Several studies have provided evidence that complex relationships between autophagic and apoptotic cell death pathways occur in cancer and virus-infected cells. Previously, we demonstrated that infection of macrophages with Moscow strain of ectromelia virus (ECTV-MOS) induces apoptosis under in vitro and in vivo conditions. Here, we found that autophagy was induced in RAW 264.7 cells during infection with ECTV-MOS. Silencing of beclin 1, an autophagy-related gene, reduced the percentage of late apoptotic cells in virus-infected RAW 264.7 macrophages. Pharmacological modulation of autophagy by wortmannin (inhibitor) or rapamycin (inductor) did not affect or cause increased apoptosis in ECTV-MOS-infected RAW 264.7 cells, respectively. Meantime, blocking apoptosis by a pan-caspase inhibitor, Z-VAD-FMK, increased the formation of autophagosomes in infected macrophages. Taken together, three important points arise from our study. First, autophagy may co-occur with apoptosis in RAW 264.7 cells exposed to ECTV-MOS. Second, at later stages of infection, autophagy may partially participate in the execution of macrophage cell death by enhancing apoptosis. Third, when apoptosis is blocked infected macrophages undergo increased autophagy. Our results provide new information about the relationship between autophagy and apoptosis in ECTV-MOS-infected macrophages.

  4. Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells.

    PubMed

    Chen, Hai-Lan; Yang, Jian; Fu, Yuan-Fang; Meng, Xi-Nan; Zhao, Wei-Dan; Hu, Ting-Jun

    2017-05-02

    This study was carried out to investigate the effect of total flavonoids of Spatholobus suberectus Dunn (TFSD) on PCV2 induced oxidative stress in RAW264.7 cells. Oxidative stress model was established in RAW264.7 cells by infecting with PCV2. Virus infected cells were then treated with various concentrations (25 mg/ml, 50 mg/ml and 100 mg/ml) of TFSD. The levels of oxidative stress related molecules (NO, ROS, GSH and GSSG) and activities of associated enzymes (SOD, MPO and XOD were analyzed using ultraviolet spectrophotometry, fluorescence method and commercialized detection kits. PCV2 infection induced significant increase of NO secretion, ROS generation, GSSG content, activities of both XOD and MPO, and dramatically decrease of GSH content and SOD activity in RAW264.7 cells (P < 0.05). After treating with TFSD, PCV2 induced alteration of oxidative stress related molecule levels and enzyme activities were recovered to a level similar to control. Our findings indicated that TFSD was able to regulate oxidative stress induced by PCV2 infection in RAW264.7 cells, which supports the ethnomedicinal use of this herb as an alternative or complementary therapeutic drug for reactive oxygen-associated pathologies.

  5. Anti-inflammatory effect of the water fraction from hawthorn fruit on LPS-stimulated RAW 264.7 cells

    PubMed Central

    Li, Chunmei

    2011-01-01

    The hawthorn fruit (Crataegus pinnatifida Bunge var. typica Schneider) is used as a traditional medicine in Korea. The objective of this study was to understand the mechanisms of the anti-inflammatory effects of the water fractionated portion of hawthorn fruit on a lipopolysaccharide (LPS)-stimulated RAW 264.7 cellular model. The level of nitric oxide (NO) production in the water fraction and LPS-treated RAW 264.7 cells were determined with an ELISA. The cytotoxicity of the water fraction and LPS was measured with an MTT assay. Expression of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin 6 (IL-6), and interleukin 1β (IL-1β) mRNA were analyzed with a reverse transcription polymerase chain reaction (RT-PCR). The water fraction of hawthorn fruit was determined to be safe and significantly inhibited NO production in LPS-stimulated RAW 264.7 cells and suppressed COX-2, TNF-α, IL-1β, and IL-6 expression. The observed anti-inflammatory effects of the water fraction of hawthorn fruit might be attributed to the down-regulation of COX-2, TNF-α, IL-1β, and IL-6 expression in LPS-stimulated RAW 264.7 cells. PMID:21556222

  6. Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages

    PubMed Central

    Wang, Xiaoli; Hu, Xiaorong; Yan, Huiqing; Ma, Zhaocheng; Deng, Xiuxin

    2016-01-01

    It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat, gum swelling, oral cavity ulcers and even fever and inflammation, which significantly impair the quality of life of a large population. Using the RAW264.7 cell line, a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse reactions. The adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program, and a sequence alignment of the homologous proteins was performed. MTT tests were used to determine the cytotoxicity of a litchi protein extract in RAW264.7 macrophages, and real-time PCR was applied to analyze the expression of inflammatory genes in the RAW264.7 cells treated with lipopolysaccharide or the litchi protein extract. The results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β, iNOS and COX-2, and the anti-inflammatory mediator HO-1 in the RAW264.7 cell line. The 14-3-3-like proteins GF14 lambda, GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects. PMID:27195125

  7. 40 CFR 264.93 - Hazardous constituents.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the ground-water quality; (vii) The potential for health risks caused by human exposure to waste... quality; (viii) The potential for health risks caused by human exposure to waste constituents; (ix) The... 264.93 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED...

  8. 40 CFR 264.171 - Condition of containers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... waste is not in good condition (e.g., severe rusting, apparent structural defects) or if it begins to... Section 264.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  9. 40 CFR 264.1059 - Standards: Delay of repair.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 264.1059 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... infeasible without a hazardous waste management unit shutdown. In such a case, repair of this equipment shall...

  10. 40 CFR 264.171 - Condition of containers.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... waste is not in good condition (e.g., severe rusting, apparent structural defects) or if it begins to... Section 264.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  11. 40 CFR 264.171 - Condition of containers.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... waste is not in good condition (e.g., severe rusting, apparent structural defects) or if it begins to... Section 264.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  12. 40 CFR 264.171 - Condition of containers.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... waste is not in good condition (e.g., severe rusting, apparent structural defects) or if it begins to... Section 264.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...

  13. [Perception of nosocomial risk among healthcare workers at "Hopital Principal" in Dakar, Senegal (survey 2004)].

    PubMed

    Chevalier, B; Margery, J; Wade, B; Ka, S; Diatta, B; Gueye, M; Mbaye, P S; Debonne, J M

    2008-12-01

    Nosocomial Infection (NI) is also observed in healthcare facilities in non-Western countries. The purpose of this report is to describe the findings of a survey undertaken to evaluate hygiene procedures implemented at the "Hopital Principal" in Dakar, Senegal and to assess perception and awareness of nosocomial risk among the hospital staff. A total of 264 healthcare workers were interviewed. Mean age was 39 years (range, 18-60) and the sex ratio was 1.3 (150 men/114 women). Sixty (22.7%) had university degrees, 106 (40.2%) had secondary school diplomas, 50 (18.9%) had attended middle school, and 13 (4.9%) had no schooling. Analysis of interview data showed that 56.1% (157/264) defined NI as infection acquired at the hospital but that only 9.8% (n=26) knew that a minimum 48-hour delay was necessary to distinguish nosocomial from community acquired infection. While understanding about NI was correlated with education level, data showed that 1 out of 3 physicians (13/39) failed to give the exact definition. Hand contact was cited as the second route of transmission. Isolation precautions were understood by 22.7% of personnel (60/264). Systematic handwashing was reported by 363% (96/264) but observation demonstrated that it was not performed properly regardless of the category of personnel. Care protocols were understood by 54.6% of persons interviewed (144/264). A hygiene-training course had been attended by 52.2% (n=138). Two thirds of the staff (69.7%: 54/264) was able to identify the hygiene nurse. Ninety-eight health care providers (37.1%) were familiar with the CLIN (Comités de Lutte contre les Infections Nosocomiales).

  14. A large-scale video codec comparison of x264, x265 and libvpx for practical VOD applications

    NASA Astrophysics Data System (ADS)

    De Cock, Jan; Mavlankar, Aditya; Moorthy, Anush; Aaron, Anne

    2016-09-01

    Over the last years, we have seen exciting improvements in video compression technology, due to the introduction of HEVC and royalty-free coding specifications such as VP9. The potential compression gains of HEVC over H.264/AVC have been demonstrated in different studies, and are usually based on the HM reference software. For VP9, substantial gains over H.264/AVC have been reported in some publications, whereas others reported less optimistic results. Differences in configurations between these publications make it more difficult to assess the true potential of VP9. Practical open-source encoder implementations such as x265 and libvpx (VP9) have matured, and are now showing high compression gains over x264. In this paper, we demonstrate the potential of these encoder imple- mentations, with settings optimized for non-real-time random access, as used in a video-on-demand encoding pipeline. We report results from a large-scale video codec comparison test, which includes x264, x265 and libvpx. A test set consisting of a variety of titles with varying spatio-temporal characteristics from our catalog is used, resulting in tens of millions of encoded frames, hence larger than test sets previously used in the literature. Re- sults are reported in terms of PSNR, SSIM, MS-SSIM, VIF and the recently introduced VMAF quality metric. BD-rate calculations show that using x265 and libvpx vs. x264 can lead to significant bitrate savings for the same quality. x265 outperforms libvpx in most cases, but the performance gap narrows (or even reverses) at the higher resolutions.

  15. LPS receptor CD14 participates in release of TNF-alpha in RAW 264.7 and peritoneal cells but not in kupffer cells.

    PubMed

    Lichtman, S N; Wang, J; Lemasters, J J

    1998-07-01

    Lipopolysaccharide (LPS) is a bacterial polymer that stimulates macrophages to release tumor necrosis factor-alpha (TNF-alpha). In macrophages (RAW 264.7 and peritoneal cells), LPS binds to the CD14 surface receptor as the first step toward signaling. Liver macrophages, Kupffer cells, are the most numerous fixed-tissue macrophage in the body. The presence of CD14 on Kupffer cells and its role in LPS stimulation of TNF-alpha were examined. TNF-alpha release by Kupffer cells after LPS stimulation was the same in the presence and absence of serum. RAW 264.7 and peritoneal cells, which utilize the CD14 receptor, released significantly less TNF-alpha after LPS stimulation in the absence of serum because of the absence of LPS-binding protein. Phosphatidylinositol-phospholipase C treatment, which cleaves the CD14 receptor, decreased LPS-stimulated TNF-alpha release by RAW 264.7 cells but not by Kupffer cells. Deacylated LPS (dLPS) competes with LPS at the CD14 receptor when incubated in a ratio of 100:1 (dLPS/LPS). Such competition blocked LPS-stimulated TNF-alpha release from RAW 264.7 cells but not from Kupffer cells. Western and fluorescence-activated cell sorter analysis directly demonstrated the presence of CD14 on RAW 264.7 cells and murine peritoneal cells but showed only minimal amounts of CD14 in murine Kupffer cells. LPS stimulation did not increase the amount of CD14 detectable on mouse Kupffer cells. CD14 expression is very low in Kupffer cells, and LPS-stimulated TNF-alpha release is independent of CD14 in these cells.

  16. 40 CFR 264.31 - Design and operation of facility.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....31 Section 264.31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...-sudden release of hazardous waste or hazardous waste constituents to air, soil, or surface water which...

  17. 40 CFR 264.31 - Design and operation of facility.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....31 Section 264.31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...-sudden release of hazardous waste or hazardous waste constituents to air, soil, or surface water which...

  18. 40 CFR 264.31 - Design and operation of facility.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....31 Section 264.31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...-sudden release of hazardous waste or hazardous waste constituents to air, soil, or surface water which...

  19. 40 CFR 264.31 - Design and operation of facility.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....31 Section 264.31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES...-sudden release of hazardous waste or hazardous waste constituents to air, soil, or surface water which...

  20. 40 CFR 264.344 - Hazardous waste incinerator permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... initial introduction of hazardous waste to the incinerator and ending with initiation of the trial burn... standards of § 264.343, based on the Regional Administrator's engineering judgment. The Regional... Regional Administrator's engineering judgement. (4) For the remaining duration of the permit, the operating...

  1. 40 CFR 264.149 - Use of State-required mechanisms.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....149 Section 264.149 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... where EPA is administering the requirements of this subpart but where the State has hazardous waste...

  2. 40 CFR 264.197 - Closure and post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 264.197 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... as hazardous waste, unless § 261.3(d) of this chapter applies. The closure plan, closure activities...

  3. 40 CFR 264.190 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Tank Systems § 264... use tank systems for storing or treating hazardous waste except as otherwise provided in paragraphs (a... treat hazardous waste which contains no free liquids and are situated inside a building with an...

  4. Coronary angiogram video compression for remote browsing and archiving applications.

    PubMed

    Ouled Zaid, Azza; Fradj, Bilel Ben

    2010-12-01

    In this paper, we propose a H.264/AVC based compression technique adapted to coronary angiograms. H.264/AVC coder has proven to use the most advanced and accurate motion compensation process, but, at the cost of high computational complexity. On the other hand, analysis of coronary X-ray images reveals large areas containing no diagnostically important information. Our contribution is to exploit the energy characteristics in slice equal size regions to determine the regions with relevant information content, to be encoded using the H.264 coding paradigm. The others regions, are compressed using fixed block motion compensation and conventional hard-decision quantization. Experiments have shown that at the same bitrate, this procedure reduces the H.264 coder computing time of about 25% while attaining the same visual quality. A subjective assessment, based on the consensus approach leads to a compression ratio of 30:1 which insures both a diagnostic adequacy and a sufficient compression in regards to storage and transmission requirements. Copyright © 2010 Elsevier Ltd. All rights reserved.

  5. Segmentation-driven compound document coding based on H.264/AVC-INTRA.

    PubMed

    Zaghetto, Alexandre; de Queiroz, Ricardo L

    2007-07-01

    In this paper, we explore H.264/AVC operating in intraframe mode to compress a mixed image, i.e., composed of text, graphics, and pictures. Even though mixed contents (compound) documents usually require the use of multiple compressors, we apply a single compressor for both text and pictures. For that, distortion is taken into account differently between text and picture regions. Our approach is to use a segmentation-driven adaptation strategy to change the H.264/AVC quantization parameter on a macroblock by macroblock basis, i.e., we deviate bits from pictorial regions to text in order to keep text edges sharp. We show results of a segmentation driven quantizer adaptation method applied to compress documents. Our reconstructed images have better text sharpness compared to straight unadapted coding, at negligible visual losses on pictorial regions. Our results also highlight the fact that H.264/AVC-INTRA outperforms coders such as JPEG-2000 as a single coder for compound images.

  6. Evaluation of the anti-inflammatory effects of phloretin and phlorizin in lipopolysaccharide-stimulated mouse macrophages.

    PubMed

    Chang, Wei-Tien; Huang, Wen-Chung; Liou, Chian-Jiun

    2012-09-15

    Many reports suggest that phloretin and phlorizin have antioxidant properties and can inhibit glucose transportation, the anti-inflammatory effects and mechanism of phloretin and phlorizin remain unclear. This study aims to evaluate the anti-inflammatory effects of phloretin and phlorizin in LPS-stimulated murine RAW264.7 macrophages. RAW264.7 cells were pretreated with various concentrations of phloretin or phlorizin (3-100 μM) and cell inflammatory responses were induced with LPS. Pretreated with 10 μM phloretin significantly inhibited the levels of NO, PGE(2), IL-6, TNF-α, iNOS and COX-2. Furthermore, it was demonstrated that phloretin suppressed the nuclear translocation of NF-κB subunit p65 proteins, and decreased phosphorylation in MAPK pathways. Surprisingly, phlorizin did not suppress the inflammatory response in LPS-stimulated RAW264.7 cells. These results suggest that phloretin has an anti-inflammatory effect that reduces levels of proinflammatory cytokines and mediators in RAW264.7 cells. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells.

    PubMed

    Shi, Dazhi; Xu, Mengyi; Ren, Mengyue; Pan, Enshan; Luo, Chaohua; Zhang, Wei; Tang, Qingfa

    2017-01-01

    This study aimed to explore the immunoregulatory effect of flavonoids of blueberry ( Vaccinium corymbosum L.) leaves (FBL). The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of FBL were investigated in lipopolysaccharide- (LPS-) induced RAW 264.7 cells. According to the results of UPLC/Q-Tof-MS, nine flavonoids of blueberry leaves were identified. FBL showed a significant reduction in the production of TNF- α in LPS-stimulated RAW 264.7 cells. FBL significantly decreased the expression of NF- κ B p65 and P-NF- κ B p65 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Our study showed the immunoregulatory effect of FBL through the suppression of TNF- α via the NF- κ B signal pathway.

  8. Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells

    PubMed Central

    Shi, Dazhi; Xu, Mengyi; Pan, Enshan; Luo, Chaohua

    2017-01-01

    Objective This study aimed to explore the immunoregulatory effect of flavonoids of blueberry (Vaccinium corymbosum L.) leaves (FBL). Methods The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of FBL were investigated in lipopolysaccharide- (LPS-) induced RAW 264.7 cells. Results According to the results of UPLC/Q-Tof-MS, nine flavonoids of blueberry leaves were identified. FBL showed a significant reduction in the production of TNF-α in LPS-stimulated RAW 264.7 cells. FBL significantly decreased the expression of NF-κB p65 and P-NF-κB p65 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Conclusion Our study showed the immunoregulatory effect of FBL through the suppression of TNF-α via the NF-κB signal pathway. PMID:29445755

  9. The Nuclear Orphan Receptor NR4A1 is Involved in the Apoptotic Pathway Induced by LPS and Simvastatin in RAW 264.7 Macrophages.

    PubMed

    Kim, Yong Chan; Song, Seok Bean; Lee, Sang Kyu; Park, Sang Min; Kim, Young Sang

    2014-04-01

    Macrophage death plays a role in several physiological and inflammatory pathologies such as sepsis and arthritis. In our previous work, we showed that simvastatin triggers cell death in LPS-activated RAW 264.7 mouse macrophage cells through both caspase-dependent and independent apoptotic pathways. Here, we show that the nuclear orphan receptor NR4A1 is involved in a caspase-independent apoptotic process induced by LPS and simvastatin. Simvastatin-induced NR4A1 expression in RAW 264.7 macrophages and ectopic expression of a dominant-negative mutant form of NR4A1 effectively suppressed both DNA fragmentation and the disruption of mitochondrial membrane potential (MMP) during LPS- and simvastatin-induced apoptosis. Furthermore, apoptosis was accompanied by Bcl-2-associated X protein (Bax) translocation to the mitochondria. Our findings suggest that NR4A1 expression and mitochondrial translocation of Bax are related to simvastatin-induced apoptosis in LPS-activated RAW 264.7 macrophages.

  10. Association of the I264T Variant in the Sulfide Quinone Reductase-Like (SQRDL) Gene with Osteoporosis in Korean Postmenopausal Women

    PubMed Central

    Park, Eunkuk; Kim, Bo-Young; Choi, Vit-Na; Yoo, Young-Hyun; Kim, Bom-Taeck; Jeong, Seon-Yong

    2015-01-01

    To identify novel susceptibility variants for osteoporosis in Korean postmenopausal women, we performed a genome-wide association analysis of 1180 nonsynonymous single nucleotide polymorphisms (nsSNPs) in 405 individuals with osteoporosis and 722 normal controls of the Korean Association Resource cohort. A logistic regression analysis revealed 72 nsSNPs that showed a significant association with osteoporosis (p<0.05). The top 10 nsSNPs showing the lowest p-values (p = 5.2×10-4–8.5×10-3) were further studied to investigate their effects at the protein level. Based on the results of an in silico prediction of the protein’s functional effect based on amino acid alterations and a sequence conservation evaluation of the amino acid residues at the positions of the nsSNPs among orthologues, we selected one nsSNP in the SQRDL gene (rs1044032, SQRDL I264T) as a meaningful genetic variant associated with postmenopausal osteoporosis. To assess whether the SQRDL I264T variant played a functional role in the pathogenesis of osteoporosis, we examined the in vitro effect of the nsSNP on bone remodeling. Overexpression of the SQRDL I264T variant in the preosteoblast MC3T3-E1 cells significantly increased alkaline phosphatase activity, mineralization, and the mRNA expression of osteoblastogenesis markers, Runx2, Sp7, and Bglap genes, whereas the SQRDL wild type had no effect or a negative effect on osteoblast differentiation. Overexpression of the SQRDL I264T variant did not affect osteoclast differentiation of the primary-cultured monocytes. The known effects of hydrogen sulfide (H2S) on bone remodeling may explain the findings of the current study, which demonstrated the functional role of the H2S-catalyzing enzyme SQRDL I264T variant in osteoblast differentiation. In conclusion, the results of the statistical and experimental analyses indicate that the SQRDL I264T nsSNP may be a significant susceptibility variant for osteoporosis in Korean postmenopausal women that is involved in osteoblast differentiation. PMID:26258864

  11. RCRA/UST, superfund, and EPCRA hotline training module. Introduction to: Treatment, storage, and disposal facilities (40 CFR parts 264/265, subparts A-E) updated as of July 1995

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1995-11-01

    The module presents an overview of the general treatment, storage, and disposal facility (TSDF) standards found in 40 CFR parts 264/265, subparts A through E. It identifies and explains each exclusion from parts 264/265, and provides definitions of excluded units, such as wastewater treatment unit and elementary neutralization unit. It locates and describes the requirements for waste analysis and personnel training. It also describes the purpose of a contingency plan and lists the emergency notification procedures. It describes manifest procedures and responsibilities, and lists the unmanifested waste reporting requirements.

  12. Draft Genome Sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a Citrate-Fermenting Strain.

    PubMed

    Zuljan, Federico; Espariz, Martín; Blancato, Victor S; Esteban, Luis; Alarcón, Sergio; Magni, Christian

    2016-02-04

    We report the draft genome sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a natural strain isolated from artisanal cheese from northwest Argentina. L. lactis subsp. lactis bv. diacetylactis is one of the most important microorganisms used as starter culture around the world. The CRL264 strain constitutes a model microorganism in the studies on the generation of aroma compounds (diacetyl, acetoin, and 2,3-butanediol) by lactic acid bacteria. Our genome analysis shows similar genetic organization to other available genomes of L. lactis bv. diacetylactis strains. Copyright © 2016 Zuljan et al.

  13. 40 CFR 264.1055 - Standards: Sampling connection systems.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Standards: Sampling connection systems... FACILITIES Air Emission Standards for Equipment Leaks § 264.1055 Standards: Sampling connection systems. (a) Each sampling connection system shall be equipped with a closed-purge, closed-loop, or closed-vent...

  14. 40 CFR 264.1055 - Standards: Sampling connection systems.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Standards: Sampling connection systems... FACILITIES Air Emission Standards for Equipment Leaks § 264.1055 Standards: Sampling connection systems. (a) Each sampling connection system shall be equipped with a closed-purge, closed-loop, or closed-vent...

  15. 40 CFR 264.254 - Monitoring and inspection.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... in the case of existing portions of piles exempt from § 264.251(a)) and cover systems (e.g., membranes, sheets, or coatings) must be inspected for uniformity, damage, and imperfections (e.g., holes, cracks, thin spots, or foreign materials). Immediately after construction or installation: (1) Synthetic...

  16. 40 CFR 264.254 - Monitoring and inspection.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... in the case of existing portions of piles exempt from § 264.251(a)) and cover systems (e.g., membranes, sheets, or coatings) must be inspected for uniformity, damage, and imperfections (e.g., holes, cracks, thin spots, or foreign materials). Immediately after construction or installation: (1) Synthetic...

  17. 40 CFR 264.254 - Monitoring and inspection.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in the case of existing portions of piles exempt from § 264.251(a)) and cover systems (e.g., membranes, sheets, or coatings) must be inspected for uniformity, damage, and imperfections (e.g., holes, cracks, thin spots, or foreign materials). Immediately after construction or installation: (1) Synthetic...

  18. 40 CFR 264.254 - Monitoring and inspection.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... in the case of existing portions of piles exempt from § 264.251(a)) and cover systems (e.g., membranes, sheets, or coatings) must be inspected for uniformity, damage, and imperfections (e.g., holes, cracks, thin spots, or foreign materials). Immediately after construction or installation: (1) Synthetic...

  19. 40 CFR 264.254 - Monitoring and inspection.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... in the case of existing portions of piles exempt from § 264.251(a)) and cover systems (e.g., membranes, sheets, or coatings) must be inspected for uniformity, damage, and imperfections (e.g., holes, cracks, thin spots, or foreign materials). Immediately after construction or installation: (1) Synthetic...

  20. 40 CFR 264.113 - Closure; time allowed for closure.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....113 Section 264.113 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES... the final volume of hazardous wastes, or the final volume of non-hazardous wastes if the owner or...

  1. 18 CFR 284.264 - Terms and conditions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., DEPARTMENT OF ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.264 Terms and...

  2. 40 CFR 264.1088 - Inspection and monitoring requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Inspection and monitoring requirements... FACILITIES Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1088 Inspection and monitoring requirements. (a) The owner or operator shall inspect and monitor air emission control equipment...

  3. 40 CFR 264.1089 - Recordkeeping requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1089 Recordkeeping... this section. (d) The owner or operator of containers using Container Level 3 air emission controls in... container is or would be operating at capacity or the highest level reasonably expected to occur. (ii) If a...

  4. 40 CFR 264.1089 - Recordkeeping requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1089 Recordkeeping... this section. (d) The owner or operator of containers using Container Level 3 air emission controls in... container is or would be operating at capacity or the highest level reasonably expected to occur. (ii) If a...

  5. 40 CFR 264.1088 - Inspection and monitoring requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Inspection and monitoring requirements... FACILITIES Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1088 Inspection and monitoring requirements. (a) The owner or operator shall inspect and monitor air emission control equipment...

  6. 40 CFR 264.1088 - Inspection and monitoring requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Inspection and monitoring requirements... FACILITIES Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1088 Inspection and monitoring requirements. (a) The owner or operator shall inspect and monitor air emission control equipment...

  7. 40 CFR 264.1088 - Inspection and monitoring requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Inspection and monitoring requirements... FACILITIES Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1088 Inspection and monitoring requirements. (a) The owner or operator shall inspect and monitor air emission control equipment...

  8. 40 CFR 264.1088 - Inspection and monitoring requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Inspection and monitoring requirements... FACILITIES Air Emission Standards for Tanks, Surface Impoundments, and Containers § 264.1088 Inspection and monitoring requirements. (a) The owner or operator shall inspect and monitor air emission control equipment...

  9. 40 CFR 264.55 - Emergency coordinator.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Contingency Plan and Emergency Procedures § 264.55 Emergency coordinator. At all times, there must be at least...'s contingency plan, all operations and activities at the facility, the location and characteristics... addition, this person must have the authority to commit the resources needed to carry out the contingency...

  10. Nanosized silver (II) pyridoxine complex to cause greater inflammatory response and less cytotoxicity to RAW264.7 macrophage cells

    NASA Astrophysics Data System (ADS)

    Paul, Avijit; Ju, Hee; Rangasamy, Sabarinathan; Shim, Yumi; Song, Joon Myong

    2015-03-01

    With advancements in nanotechnology, silver has been engineered into a nanometre size and has attracted great research interest for use in the treatment of wounds. Silver nanoparticles (AgNPs) have emerged as a potential alternative to conventional antibiotics because of their potential antimicrobial property. However, AgNPs also induce cytotoxicity, generate reactive oxygen species (ROS), and cause mitochondrial damage to human cells. Pyridoxine possesses antioxidant and cell proliferation activity. Therefore, in the present investigation, a nanosilver-pyridoxine complex (AgPyNP) was synthesized, and its cytotoxicity and immune response was compared with AgNPs in macrophage RAW264.7 cells. Results revealed that AgPyNPs showed less cytotoxicity compared with AgNPs by producing a smaller amount of ROS in RAW264.7 cells. Surprisingly, however, AgPyNPs caused macrophage RAW264.7 cells to secrete a larger amount of interleukin-8 (IL-8) and generate a more active inflammatory response compared to AgNPs. It activated TNF-α, NF-κB p65, and NF-κB p50 to generate a more vigorous immune protection that produces a greater amount of IL-8 compared to AgNPs. Overall findings indicate that AgPyNPs exhibited less cytotoxicity and evoked a greater immune response in macrophage RAW264.7 cells. Thus, it can be used as a better wound-healing agent than AgNPs.

  11. 40 CFR 264.97 - General ground-water monitoring requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... collection of ground-water samples. The annular space (i.e., the space between the bore hole and well casing... 40 Protection of Environment 27 2013-07-01 2013-07-01 false General ground-water monitoring... FACILITIES Releases From Solid Waste Management Units § 264.97 General ground-water monitoring requirements...

  12. 40 CFR 264.37 - Arrangements with local authorities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Arrangements with local authorities... FACILITIES Preparedness and Prevention § 264.37 Arrangements with local authorities. (a) The owner or... familiarize local hospitals with the properties of hazardous waste handled at the facility and the types of...

  13. 40 CFR 264.37 - Arrangements with local authorities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Arrangements with local authorities... FACILITIES Preparedness and Prevention § 264.37 Arrangements with local authorities. (a) The owner or... familiarize local hospitals with the properties of hazardous waste handled at the facility and the types of...

  14. 27 CFR 25.264 - Transfer between breweries.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., DEPARTMENT OF THE TREASURY LIQUORS BEER Beer Concentrate § 25.264 Transfer between breweries. (a) Authorized transfers. A brewer may remove from the brewery, without payment of tax, concentrate produced from beer for... transfer. The invoice will be clearly marked to indicate that concentrate produced from beer is being...

  15. 40 CFR 266.102 - Permit standards for burners.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... subpart G (Closure and post-closure), §§ 264.111-264.115; (viii) In subpart H (Financial requirements... prescribed in paragraph (e)(6) of this section; (C) Appropriate controls of the hazardous waste firing system...; (C) Appropriate controls on operation and maintenance of the hazardous waste firing system and any...

  16. 40 CFR 266.102 - Permit standards for burners.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... subpart G (Closure and post-closure), §§ 264.111-264.115; (viii) In subpart H (Financial requirements... prescribed in paragraph (e)(6) of this section; (C) Appropriate controls of the hazardous waste firing system...; (C) Appropriate controls on operation and maintenance of the hazardous waste firing system and any...

  17. 40 CFR 266.102 - Permit standards for burners.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... subpart G (Closure and post-closure), §§ 264.111-264.115; (viii) In subpart H (Financial requirements... prescribed in paragraph (e)(6) of this section; (C) Appropriate controls of the hazardous waste firing system...; (C) Appropriate controls on operation and maintenance of the hazardous waste firing system and any...

  18. 40 CFR 266.102 - Permit standards for burners.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... subpart G (Closure and post-closure), §§ 264.111-264.115; (viii) In subpart H (Financial requirements... prescribed in paragraph (e)(6) of this section; (C) Appropriate controls of the hazardous waste firing system...; (C) Appropriate controls on operation and maintenance of the hazardous waste firing system and any...

  19. 40 CFR 266.102 - Permit standards for burners.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... subpart G (Closure and post-closure), §§ 264.111-264.115; (viii) In subpart H (Financial requirements... prescribed in paragraph (e)(6) of this section; (C) Appropriate controls of the hazardous waste firing system...; (C) Appropriate controls on operation and maintenance of the hazardous waste firing system and any...

  20. 27 CFR 25.264 - Transfer between breweries.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., DEPARTMENT OF THE TREASURY ALCOHOL BEER Beer Concentrate § 25.264 Transfer between breweries. (a) Authorized transfers. A brewer may remove from the brewery, without payment of tax, concentrate produced from beer for... transfer. The invoice will be clearly marked to indicate that concentrate produced from beer is being...

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