Sample records for bohrium 271

  1. Road dust and its effect on human health: a literature review

    PubMed Central

    2018-01-01

    The purpose of this study was to determine the effects of road dust on human health. A PubMed search was used to extract references that included the words “road dust” and “health” or “fugitive dust” and “health” in the title or abstract. A total of 46 references were extracted and selected for review after the primary screening of 949 articles. The respiratory system was found to be the most affected system in the human body. Lead, platinum-group elements (platinum, rhodium, and bohrium), aluminum, zinc, vanadium, and polycyclic aromatic hydrocarbons were the components of road dust that were most frequently referenced in the articles reviewed. Road dust was found to have harmful effects on the human body, especially on the respiratory system. To determine the complex mechanism of action of various components of road dust on the human body and the results thereof, the authors recommend a further meta-analysis and extensive risk-assessment research into the health impacts of dust exposure. PMID:29642653

  2. 30. BUILDING NO.S 271K AND 271L, VIEW LOOKING SOUTH AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. BUILDING NO.S 271-K AND 271-L, VIEW LOOKING SOUTH AT BACK OF BUILDING NO. 271-L (LEFT), 271-K (MIDDLE) AND ROOF OF BUILDING NO. 271-I (VISIBLE OVER WALKWAY ON RIGHT). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  3. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells.

    PubMed

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-07-29

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells' tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC.

  4. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells

    PubMed Central

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-01-01

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells’ tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC. PMID:27469492

  5. CD271 Defines a Stem Cell-Like Population in Hypopharyngeal Cancer

    PubMed Central

    Imai, Takayuki; Tamai, Keiichi; Oizumi, Sayuri; Oyama, Kyoko; Yamaguchi, Kazunori; Sato, Ikuro; Satoh, Kennichi; Matsuura, Kazuto; Saijo, Shigeru; Sugamura, Kazuo; Tanaka, Nobuyuki

    2013-01-01

    Cancer stem cells contribute to the malignant phenotypes of a variety of cancers, but markers to identify human hypopharyngeal cancer (HPC) stem cells remain poorly understood. Here, we report that the CD271+ population sorted from xenotransplanted HPCs possesses an enhanced tumor-initiating capability in immunodeficient mice. Tumors generated from the CD271+ cells contained both CD271+ and CD271− cells, indicating that the population could undergo differentiation. Immunohistological analyses of the tumors revealed that the CD271+ cells localized to a perivascular niche near CD34+ vasculature, to invasive fronts, and to the basal layer. In accordance with these characteristics, a stemness marker, Nanog, and matrix metalloproteinases (MMPs), which are implicated in cancer invasion, were significantly up-regulated in the CD271+ compared to the CD271− cell population. Furthermore, using primary HPC specimens, we demonstrated that high CD271 expression was correlated with a poor prognosis for patients. Taken together, our findings indicate that CD271 is a novel marker for HPC stem-like cells and for HPC prognosis. PMID:23626764

  6. Comparative Effect of an Addition of a Surface Term to Woods-Saxon Potential on Thermodynamics of a Nucleon

    NASA Astrophysics Data System (ADS)

    Lütfüoğlu, B. C.

    2018-01-01

    In this study, we reveal the difference between Woods-Saxon (WS) and Generalized Symmetric Woods-Saxon (GSWS) potentials in order to describe the physical properties of a nucleon, by means of solving Schrödinger equation for the two potentials. The additional term squeezes the WS potential well, which leads an upward shift in the spectrum, resulting in a more realistic picture. The resulting GSWS potential does not merely accommodate extra quasi bound states, but also has modified bound state spectrum. As an application, we apply the formalism to a real problem, an α particle confined in Bohrium-270 nucleus. The thermodynamic functions Helmholtz energy, entropy, internal energy, specific heat of the system are calculated and compared for both wells. The internal energy and the specific heat capacity increase as a result of upward shift in the spectrum. The shift of the Helmholtz free energy is a direct consequence of the shift of the spectrum. The entropy decreases because of a decrement in the number of available states. Supported by the Turkish Science and Research Council (TÜBİTAK) and Akdeniz University

  7. 48 CFR 719.271-5 - Cognizant technical officers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... officers. 719.271-5 Section 719.271-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-5 Cognizant technical officers... cognizant technical officers can affect the opportunity of small business to participate equitably...

  8. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  9. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  10. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  11. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  12. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  13. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State...

  14. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.11 Requirements for transporters of hazardous wastes. (a) The State...

  15. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.11 Requirements for transporters of hazardous wastes. (a) The State...

  16. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State...

  17. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State...

  18. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations § 115.271...

  19. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations § 115.271...

  20. 28 CFR 115.271 - Criminal and administrative agency investigations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Criminal and administrative agency investigations. 115.271 Section 115.271 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Community Confinement Facilities Investigations § 115.271...

  1. 47 CFR 80.271 - Technical requirements for portable survival craft radiotelephone transceivers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... craft radiotelephone transceivers. 80.271 Section 80.271 Telecommunication FEDERAL COMMUNICATIONS... Authorization for Compulsory Ships § 80.271 Technical requirements for portable survival craft radiotelephone transceivers. (a) Portable survival craft radiotelephone transceivers must comply with the following: (1) The...

  2. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 22 2012-07-01 2012-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must...

  3. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must...

  4. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  5. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  6. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  7. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  8. 47 CFR 1.736 - Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Complaints filed pursuant to 47 U.S.C. 271(d)(6)(B). (a) Where a complaint is filed pursuant to 47 U.S.C. 271... deadline contained in 47 U.S.C. 271(d)(6)(B) in the following manner: (1) The complainant shall so indicate... filed pursuant to 47 U.S.C. 271(d)(6)(B) will not be entertained by the Commission staff subsequent to...

  9. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  10. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  11. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  12. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  13. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  14. 48 CFR 719.271 - Agency program direction and operation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Agency program direction and operation. 719.271 Section 719.271 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271 Agency program direction and...

  15. 48 CFR 719.271 - Agency program direction and operation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Agency program direction and operation. 719.271 Section 719.271 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271 Agency program direction and...

  16. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  17. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  18. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  19. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  20. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Fire-extinguishing system materials. 121.271 Section 121.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each...

  1. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission may... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Waiver. 284.271 Section 284.271 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF...

  2. 14 CFR 271.7 - Subsidy payout formula.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Subsidy payout formula. 271.7 Section 271.7 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.7 Subsidy...

  3. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... activities. 719.271-4 Section 719.271-4 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities. In order for the agency small business program to be effective, the active support of top management...

  4. 49 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Purpose. 27.1 Section 27.1 Transportation Office of the Secretary of Transportation NONDISCRIMINATION ON THE BASIS OF DISABILITY IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE General § 27.1 Purpose. The purpose of this part is to...

  5. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used in...

  6. CD271+ cells are diagnostic and prognostic and exhibit elevated MAPK activity in SHH medulloblastoma.

    PubMed

    Liang, Lisa; Morrison, Ludivine Coudière; Tatari, Nazanin; Stromecki, Margaret; Fresnoza, Agnes; Porter, Christopher J; Del Bigio, Marc R; Hawkins, Cynthia; Chan, Jennifer A; Ryken, Timothy C; Taylor, Michael D; Ramaswamy, Vijay; Werbowetski-Ogilvie, Tamra E

    2018-06-21

    The extensive heterogeneity both between and within the medulloblastoma (MB) subgroups underscores a critical need for variant-specific biomarkers and therapeutic strategies. We previously identified a role for the CD271/p75 neurotrophin receptor (p75NTR) in regulating stem/progenitor cells in the SHH MB subgroup. Here we demonstrate the utility of CD271 as a novel diagnostic and prognostic marker for SHH MB using immunohistochemical analysis and transcriptome data across 763 primary tumors. RNA sequencing of CD271+ and CD271- cells revealed molecularly distinct, co-existing cellular subsets both in vitro and in vivo. MAPK/ERK signaling was upregulated in the CD271+ population, and inhibiting this pathway reduced endogenous CD271 levels, stem/progenitor cell proliferation, and cell survival as well as cell migration in vitro. Treatment with the MEK inhibitor selumetinib extended survival and reduced CD271 levels in vivo; whereas, treatment with vismodegib, a well-known smoothened (SMO) inhibitor currently in clinical trials for the treatment of recurrent SHH medulloblastoma, had no significant effect in our models. Our study demonstrates the clinical utility of CD271 as both a diagnostic and prognostic tool for SHH MB tumors and reveals a novel role for MEK inhibitors in targeting CD271+ SHH MB cells. Copyright ©2018, American Association for Cancer Research.

  7. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  8. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  9. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  10. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  11. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances. [70 FR 73150...

  12. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  13. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 3 2010-07-01 2010-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  14. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 3 2011-07-01 2011-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  15. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 3 2013-07-01 2013-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  16. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 3 2012-07-01 2012-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  17. 40 CFR 52.271 - Malfunction, startup, and shutdown regulations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 3 2014-07-01 2014-07-01 false Malfunction, startup, and shutdown regulations. 52.271 Section 52.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF IMPLEMENTATION PLANS California § 52.271 Malfunction, startup, and shutdown regulations. (a)...

  18. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  19. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  20. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  1. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  2. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  3. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  4. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  5. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  6. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  7. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  8. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  9. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  10. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  11. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  12. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  13. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  14. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  15. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true NASA Solicitation provision and contract clause. 1823.271 Section 1823.271 Federal Acquisition Regulations System NATIONAL... Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at 1852.223-76...

  16. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Published information. 271.3 Section 271.3... AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the... the actions, and the votes taken. (c) Other published information. From time to time, other...

  17. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Rate period. 271.8 Section 271.8... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period. (a) The subsidy rate generally will be set for a 2-year period, or two consecutive 1-year periods...

  18. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Rate period. 271.8 Section 271.8... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period. (a) The subsidy rate generally will be set for a 2-year period, or two consecutive 1-year periods...

  19. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Rate period. 271.8 Section 271.8... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period. (a) The subsidy rate generally will be set for a 2-year period, or two consecutive 1-year periods...

  20. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Government Technical Monitor (GTM). 642.271 Section 642.271 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACT MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The...

  1. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  2. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 21 2011-07-01 2011-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if you...

  3. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 22 2013-07-01 2013-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if you...

  4. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 21 2014-07-01 2014-07-01 false Reporting threshold. 98.271 Section 98.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if you...

  5. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  6. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  7. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  8. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where EPA...

  9. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  10. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  11. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  12. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  13. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the following clause: Time Spent by Counselee in Counseling Process (APR 1984) The contractor agrees that no...

  14. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Requirements (AIR)-growth work 1352.271-72 Section 1352.271-72 Federal Acquisition Regulations System... Clauses 1352.271-72 Additional Item Requirements (AIR)—growth work As prescribed in 48 CFR 1371.103, insert the following clause: Additional Item Requirements (Air)—Growth Work (APR 2010) (a) This clause...

  15. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  16. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  17. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  18. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  19. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... and Disadvantaged Business Utilization (SDB). 719.271-2 Section 719.271-2 Federal Acquisition... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is... business enterprises) program. (b) SDB, headed by the Director SDB, who also serves as the Minority...

  20. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to human...

  1. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to human...

  2. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the Board... of 31 U.S.C. 751. For purposes of the regulations in this part and 4 CFR part 28, a simple majority...

  3. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and copied during normal business hours at the following locations: Office of State Programs... 29 Labor 9 2010-07-01 2010-07-01 false Where the plan may be inspected. 1952.271 Section 1952.271..., DEPARTMENT OF LABOR (CONTINUED) APPROVED STATE PLANS FOR ENFORCEMENT OF STATE STANDARDS Vermont § 1952.271...

  4. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-87 Changes—ship repair. As prescribed in 48 CFR 1371.118,...

  5. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  6. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  7. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  8. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  9. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Records available to the public on request. 271... RULES REGARDING AVAILABILITY OF INFORMATION § 271.5 Records available to the public on request. (a) Types of records made available. All records of the Committee that are not available under §§ 271.3 and...

  10. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Records available to the public on request. 271... RULES REGARDING AVAILABILITY OF INFORMATION § 271.5 Records available to the public on request. (a) Types of records made available. All records of the Committee that are not available under §§ 271.3 and...

  11. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  12. 48 CFR 1352.271-71 - Method of payment and invoicing instructions for ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Method of payment and invoicing instructions for ship repair. 1352.271-71 Section 1352.271-71 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-71 Method of...

  13. 30 CFR 550.271 - For what reasons will BOEM disapprove the DPP or DOCD?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false For what reasons will BOEM disapprove the DPP or DOCD? 550.271 Section 550.271 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF... Information Review and Decision Process for the Dpp Or Docd § 550.271 For what reasons will BOEM disapprove...

  14. 30 CFR 550.271 - For what reasons will BOEM disapprove the DPP or DOCD?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false For what reasons will BOEM disapprove the DPP or DOCD? 550.271 Section 550.271 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF... Information Review and Decision Process for the Dpp Or Docd § 550.271 For what reasons will BOEM disapprove...

  15. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 1984 (HSWA) include any requirement or prohibition which has taken effect under HSWA, such as: (1) All... have taken effect. Note: See §§ 264.1(f)(3), 265.1(c)(4)(ii), 271.3(b), 271.21(e)(2) and 271.121(c)(3... the recycling of hazardous wastes in the coke by-products industry 57 FR 27888 June 22, 1992. Aug. 18...

  16. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 1984 (HSWA) include any requirement or prohibition which has taken effect under HSWA, such as: (1) All... have taken effect. Note: See §§ 264.1(f)(3), 265.1(c)(4)(ii), 271.3(b), 271.21(e)(2) and 271.121(c)(3... the recycling of hazardous wastes in the coke by-products industry 57 FR 27888 June 22, 1992. Aug. 18...

  17. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 1984 (HSWA) include any requirement or prohibition which has taken effect under HSWA, such as: (1) All... have taken effect. Note: See §§ 264.1(f)(3), 265.1(c)(4)(ii), 271.3(b), 271.21(e)(2) and 271.121(c)(3... the recycling of hazardous wastes in the coke by-products industry 57 FR 27888 June 22, 1992. Aug. 18...

  18. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Francisella tularensis SCHU S4 Infection in BALB/c Mice and Cynomolgus Macaques.

    PubMed

    Grossman, Trudy H; Anderson, Michael S; Christ, David; Gooldy, Melanie; Henning, Lisa N; Heine, Henry S; Kindt, M Victoria; Lin, Winston; Siefkas-Patterson, Kaylyn; Radcliff, Anne K; Tam, Vincent H; Sutcliffe, Joyce A

    2017-08-01

    TP-271 is a novel, fully synthetic fluorocycline in development for complicated bacterial respiratory infections. TP-271 was active in vitro against a panel of 29 Francisella tularensis isolates, showing MICs against 50% and 90% of isolates of 0.25 and 0.5 μg/ml, respectively. In a mouse model of inhalational tularemia, animals were exposed by aerosol to 91 to 283 50% lethal doses (LD 50 )/mouse of F. tularensis SCHU S4. Following 21 days of once-daily intraperitoneal dosing with TP-271 at 3, 6, 12, and 18 mg/kg of body weight/day, initiating at 24 h postchallenge, survival was 80%, 100%, 100%, and 100%, respectively. When treatment was initiated at 72 h postchallenge, survival was 89%, 100%, 100%, and 100% in the 3-, 6-, 12-, and 18-mg/kg/day TP-271 groups, respectively. No mice treated with the vehicle control survived. Surviving mice treated with TP-271 showed little to no relapse during 14 days posttreatment. In a nonhuman primate model of inhalational tularemia, cynomolgus macaques received an average aerosol exposure of 1,144 CFU of F. tularensis SCHU S4. Once-daily intravenous infusion with 1 or 3 mg/kg TP-271, or vehicle control, for 21 days was initiated within 6 h of confirmed fever. All animals treated with TP-271 survived to the end of the study, with no relapse during 14 days after the last treatment, whereas no vehicle control-treated animals survived. The protection and low relapse afforded by TP-271 treatment in these studies support continued investigation of TP-271 for use in the event of aerosolized exposure to F. tularensis . Copyright © 2017 American Society for Microbiology.

  19. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Francisella tularensis SCHU S4 Infection in BALB/c Mice and Cynomolgus Macaques

    PubMed Central

    Anderson, Michael S.; Christ, David; Gooldy, Melanie; Henning, Lisa N.; Heine, Henry S.; Kindt, M. Victoria; Lin, Winston; Siefkas-Patterson, Kaylyn; Radcliff, Anne K.; Tam, Vincent H.; Sutcliffe, Joyce A.

    2017-01-01

    ABSTRACT TP-271 is a novel, fully synthetic fluorocycline in development for complicated bacterial respiratory infections. TP-271 was active in vitro against a panel of 29 Francisella tularensis isolates, showing MICs against 50% and 90% of isolates of 0.25 and 0.5 μg/ml, respectively. In a mouse model of inhalational tularemia, animals were exposed by aerosol to 91 to 283 50% lethal doses (LD50)/mouse of F. tularensis SCHU S4. Following 21 days of once-daily intraperitoneal dosing with TP-271 at 3, 6, 12, and 18 mg/kg of body weight/day, initiating at 24 h postchallenge, survival was 80%, 100%, 100%, and 100%, respectively. When treatment was initiated at 72 h postchallenge, survival was 89%, 100%, 100%, and 100% in the 3-, 6-, 12-, and 18-mg/kg/day TP-271 groups, respectively. No mice treated with the vehicle control survived. Surviving mice treated with TP-271 showed little to no relapse during 14 days posttreatment. In a nonhuman primate model of inhalational tularemia, cynomolgus macaques received an average aerosol exposure of 1,144 CFU of F. tularensis SCHU S4. Once-daily intravenous infusion with 1 or 3 mg/kg TP-271, or vehicle control, for 21 days was initiated within 6 h of confirmed fever. All animals treated with TP-271 survived to the end of the study, with no relapse during 14 days after the last treatment, whereas no vehicle control-treated animals survived. The protection and low relapse afforded by TP-271 treatment in these studies support continued investigation of TP-271 for use in the event of aerosolized exposure to F. tularensis. PMID:28559261

  20. Functional analysis of potassium channels in Kv7.2 G271V mutant causing early onset familial epilepsy.

    PubMed

    Wang, Juanjuan; Li, Yuan; Hui, Zhiyan; Cao, Min; Shi, Ruiming; Zhang, Wei; Geng, Limeng; Zhou, Xihui

    2015-08-07

    Kv7 (KCNQ) channels underlying a class of voltage-gated K+ current are best known for regulating neuronal excitability. The first glycine (G) residue in the pore helix of Kv7.2 (KCNQ2) subunit is highly conserved among different classes of Kv7 channel family. A missense mutation causing the replacement of the corresponding G residues with a valine (p.G271V) in Kv7.2 was found in a large, four-generation pedigree. Here, we set out to examine the molecular pathomechanism of G271V mutants using patch clamp technology combined with biochemical and immunocytochemical techniques in transiently transfected human embryonic kidney (HEK) 293 cells. The expression of Kv7.2 protein had the same intensity for both wild type (WT) and G271V. In transfected HEK cells, G271V mutants induced large depolarizing shifts of the conductance-voltage relationships and marked slowing of current activation kinetics compared to WT. In addition, G271V mutants abolished currents in homomeric channels, and resulted in about 50% reduction of current in Kv7.2/G271V/Kv7.3 heteromultimeric condition, indicating a more severe functional defect. To test for G271V mutant channel expression in surface membrane, we performed fluorescence confocal microscopy imaging, which revealed no differences between the mutant and WT, suggesting that G271V channels fail to open in response to depolarization even though they are present in the membrane. Furthermore, pharmacologic intervention experiments revealed that upon specific incubation of transfected HEK 293 cells expressing G271V heteromultimeric channels in presence of Kv7 channel enhancer retigabine (ezogabine), the potassium currents increased significantly, suggesting the potential of retigabine as gene-specific therapy. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. 47 CFR 0.271 - Authority delegated.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Authority delegated. 0.271 Section 0.271 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL COMMISSION ORGANIZATION Delegations of Authority Office... amended at 68 FR 11747, Mar. 12, 2003] Chief, Media Bureau ...

  2. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Discrimination prohibited. 271.9 Section 271.9 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR...

  3. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  4. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  5. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  6. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  7. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Specialized definitions. [Reserved] 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  8. 14 CFR 27.1 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Applicability. 27.1 Section 27.1 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... standards for the issue of type certificates, and changes to those certificates, for normal category...

  9. 14 CFR 27.1 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Applicability. 27.1 Section 27.1 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... standards for the issue of type certificates, and changes to those certificates, for normal category...

  10. 40 CFR 271.3 - Availability of final authorization.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... authority of the Hazardous Waste Electronic Manifest Establishment Act: (i) Shall take effect in each State.... 271.3 Section 271.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final...

  11. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  12. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  13. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  14. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  15. 48 CFR 1352.271-89 - Temporary services.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Temporary services. 1352.271-89 Section 1352.271-89 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND..., electricity, telephone service, toilet facilities, garbage removal, office space, parking places or similar...

  16. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  17. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  18. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  19. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  20. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  1. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  2. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  3. Single-Stage Preparation of Human Cartilage Grafts Generated from Bone Marrow-Derived CD271+ Mononuclear Cells.

    PubMed

    Petters, Oliver; Schmidt, Christian; Henkelmann, Ralf; Pieroh, Philipp; Hütter, Gero; Marquass, Bastian; Aust, Gabriela; Schulz, Ronny M

    2018-04-15

    Due to the limited self-healing capacity of articular cartilage, innovative, regenerative approaches are of particular interest. The use of two-stage procedures utilizing in vitro-expanded mesenchymal stromal cells (MSCs) from various cell sources requires good manufacturing practice-compliant production, a process with high demands on time, staffing, and financial resources. In contrast, one- stage procedures are directly available, but need a safe enrichment of potent MSCs. CD271 is a surface marker known to marking the majority of native MSCs in bone marrow (BM). In this study, the feasibility of generating a single-stage cartilage graft of enriched CD271 + BM-derived mononuclear cells (MNCs) without in vitro monolayer expansion from eight healthy donors was investigated. Cartilage grafts were generated by magnetic-activated cell sorting and separated cells were directly transferred into collagen type I hydrogels, followed by 3D proliferation and differentiation period of CD271 + , CD271 - , or unseparated MNCs. CD271 + MNCs showed the highest proliferation rate, cell viability, sulfated glycosaminoglycan deposition, and cartilage marker expression compared to the CD271 - or unseparated MNC fractions in 3D culture. Analysis according to the minimal criteria of the International Society for Cellular Therapy highlighted a 66.8-fold enrichment of fibroblast colony-forming units in CD271 + MNCs and the only fulfillment of the MSC marker profile compared to unseparated MNCs. In summary, CD271 + MNCs are capable of generating adequate articular cartilage grafts presenting high cell viability and notable chondrogenic matrix deposition in a CE-marked collagen type I hydrogel, which can obviate the need for an initial monolayer expansion.

  4. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... that such wastes do not present a hazard to human health or the environment. These requirements shall... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements...

  5. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... that such wastes do not present a hazard to human health or the environment. These requirements shall... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements...

  6. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... that such wastes do not present a hazard to human health or the environment. These requirements shall... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements...

  7. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  8. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  9. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  10. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  11. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  12. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Facility pest management practice standard. 205.271... MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED... Requirements § 205.271 Facility pest management practice standard. (a) The producer or handler of an organic...

  13. 48 CFR 719.271-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 719.271-1 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-1 General. The purpose of this section is to prescribe responsibilities and procedures for carrying out the small business program policy set forth in 219.270, and in...

  14. 48 CFR 719.271-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 719.271-1 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-1 General. The purpose of this section is to prescribe responsibilities and procedures for carrying out the small business program policy set forth in 219.270, and in...

  15. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the transportation and delivery of hazardous waste shipments. No other electronic signature other than... in connection with the signing of an electronic manifest. (3) Ensure that all wastes offered for... hazardous wastes. 271.10 Section 271.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED...

  16. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  17. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  18. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  19. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  20. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Rate period. 271.8 Section 271.8 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... place essential air service level; or (5) The uncertainties of the market or other circumstances warrant...

  1. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  2. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  3. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  4. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  5. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor shall...

  6. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Heads of contracting activities. 719.271-4 Section 719.271-4 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL... decisions in cases resulting from non-acceptances by their contracting officers of set-aside recommendations...

  7. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE...

  8. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE...

  9. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  10. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  11. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  12. 48 CFR 1352.271-84 - Access to the vessel.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Access to the vessel. 1352.271-84 Section 1352.271-84 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND... make reasonable arrangements to provide access for these personnel to office space, work areas, storage...

  13. 14 CFR 125.271 - Emergency and emergency evacuation duties.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Emergency and emergency evacuation duties. 125.271 Section 125.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... CERTIFICATION AND OPERATIONS: AIRPLANES HAVING A SEATING CAPACITY OF 20 OR MORE PASSENGERS OR A MAXIMUM PAYLOAD...

  14. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of forest...

  15. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall...

  16. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall...

  17. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  18. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  19. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  20. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  1. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... minimize the impacts of use as a dust suppressant on the environment. (h)(1) Unless otherwise provided in... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Requirements for used oil management. 271.26 Section 271.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID...

  2. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... minimize the impacts of use as a dust suppressant on the environment. (h)(1) Unless otherwise provided in... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Requirements for used oil management. 271.26 Section 271.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID...

  3. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... minimize the impacts of use as a dust suppressant on the environment. (h)(1) Unless otherwise provided in... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Requirements for used oil management. 271.26 Section 271.26 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID...

  4. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  5. 40 CFR 271.7 - Attorney General's statement.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Attorney General's statement. 271.7... Authorization § 271.7 Attorney General's statement. (a) Any State that seeks to administer a program under this subpart shall submit a statement from the State Attorney General (or the attorney for those State agencies...

  6. 20 CFR 404.271 - When automatic cost-of-living increases apply.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false When automatic cost-of-living increases apply. 404.271 Section 404.271 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Computing Primary Insurance Amounts Cost-Of-Living Increases § 404...

  7. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  8. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  9. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  10. 36 CFR 271.1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Definitions. 271.1 Section... BEARâ SYMBOL § 271.1 Definitions. (a) The term Smokey Bear as used in the regulations in this part means... Agriculture in cooperation with the Association of State Foresters and The Advertising Council, or any...

  11. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of forest...

  12. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Title. 1352.271-80 Section... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following clause: Title (APR 2010) (a) Title to all materials and...

  13. 36 CFR 271.8 - Consultation with Association of State Foresters and the Advertising Council.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Consultation with Association of State Foresters and the Advertising Council. 271.8 Section 271.8 Parks, Forests, and Public... Association of State Foresters and the Advertising Council. These regulations in this part have been issued...

  14. 48 CFR 1352.271-86 - Lay days.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Lay days. 1352.271-86... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-86 Lay days. As prescribed in 48 CFR 1371.117, insert the following clause: Lay Days (APR 2010) (a) A lay day is defined as an...

  15. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY THE SECRETARY OF AGRICULTURE AND GENERAL OFFICERS OF THE DEPARTMENT Delegations of Authority by the...

  16. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY THE SECRETARY OF AGRICULTURE AND GENERAL OFFICERS OF THE DEPARTMENT Delegations of Authority by the...

  17. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY BY THE SECRETARY OF AGRICULTURE AND GENERAL OFFICERS OF THE DEPARTMENT Delegations of Authority by the...

  18. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System, 20th Street and Constitution Avenue, N.W., Washington, D.C. 20551 (the pedestrian entrance is on C...

  19. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System, 20th Street and Constitution Avenue, N.W., Washington, D.C. 20551 (the pedestrian entrance is on C...

  20. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System, 20th Street and Constitution Avenue, N.W., Washington, D.C. 20551 (the pedestrian entrance is on C...

  1. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware of...

  2. Characterization of novel biomarkers in selecting for subtype specific medulloblastoma phenotypes.

    PubMed

    Liang, Lisa; Aiken, Christopher; McClelland, Robyn; Morrison, Ludivine Coudière; Tatari, Nazanin; Remke, Marc; Ramaswamy, Vijay; Issaivanan, Magimairajan; Ryken, Timothy; Del Bigio, Marc R; Taylor, Michael D; Werbowetski-Ogilvie, Tamra E

    2015-11-17

    Major research efforts have focused on defining cell surface marker profiles for characterization and selection of brain tumor stem/progenitor cells. Medulloblastoma is the most common primary malignant pediatric brain cancer and consists of 4 molecular subgroups: WNT, SHH, Group 3 and Group 4. Given the heterogeneity within and between medulloblastoma variants, surface marker profiles may be subtype-specific. Here, we employed a high throughput flow cytometry screen to identify differentially expressed cell surface markers in self-renewing vs. non-self-renewing SHH medulloblastoma cells. The top 25 markers were reduced to 4, CD271/p75NTR/NGFR, CD106/VCAM1, EGFR and CD171/NCAM-L1, by evaluating transcript levels in SHH tumors relative to samples representing the other variants. However, only CD271/p75NTR/NGFR and CD171/NCAM-L1 maintain differential expression between variants at the protein level. Functional characterization of CD271, a low affinity neurotrophin receptor, in cell lines and primary cultures suggested that CD271 selects for lower self-renewing progenitors or stem cells. Moreover, CD271 levels were negatively correlated with expression of SHH pathway genes. Our study reveals a novel role for CD271 in SHH medulloblastoma and suggests that targeting CD271 pathways could lead to the design of more selective therapies that lessen the broad impact of current treatments on developing nervous systems.

  3. Functional characteristics of a novel SMAD4 mutation from thoracic aortic aneurysms (TAA).

    PubMed

    Wu, Lifei

    2017-09-10

    SMAD4 is as an essential mediator of the transforming growth factor β (TGF-β) signaling pathway, and dysregulated TGF-β signaling is linked with thoracic aortic aneurysms (TAAs). In this study, we functionally characterized the Smad4 S271N mutation (the mutation c. 812G>A in Smad4 results in the amino acid substitution Ser271Asn) that was isolated from TAA individuals. We first constructed wild-type human Smad4 and Smad4 S271N plasmids. These constructs were then transiently transfected into HEK293T cells, and subsequent real-time PCR and western blotting demonstrated that wild-type Smad4 and Smad4 S271N were successfully expressed in 293T cells. We found that HEK293T cells overexpressing Smad4 S271N showed a strong increase in both cytoplasmic and nuclear Smad4 protein levels in response to TGF-β1. Although TGF-β signaling was the same in wild-type Smad4- and Smad4 S271N-transfected cells following TGF-β1 exposure, interestingly, we observed that transient Smad4 S271N expression in HEK293T cells caused a significant basal activation of TGF-β signaling. These results indicated that Smad4 may not directly induce TAA; rather it may contribute to TAA in combination with other risk factors. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of the...

  5. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of the...

  6. Aggressive pituitary adenomas occurring in young patients in a large Polynesian kindred with a germline R271W mutation in the AIP gene.

    PubMed

    Jennings, Juliet E; Georgitsi, Marianthi; Holdaway, Ian; Daly, Adrian F; Tichomirowa, Maria; Beckers, Albert; Aaltonen, Lauri A; Karhu, Auli; Cameron, Fergus J

    2009-11-01

    Mutations in the aryl hydrocarbon receptor-interacting protein (AIP) were recently shown to confer a pituitary adenoma predisposition in patients with familial isolated pituitary adenomas (FIPA). We report a large Samoan FIPA kindred from Australia/New Zealand with an R271W mutation that was associated with aggressive pituitary tumors. Case series with germline screening of AIP and haplotype analyses among R271W families. This previously unreported kindred consisted of three affected individuals that either presented with or had first symptoms of a pituitary macroadenoma in late childhood or adolescence. The index case, a 15-year-old male with incipient gigantism and his maternal aunt, had somatotropinomas, and the maternal uncle of the index case had a prolactinoma. All tumors were large (15, 40, and 60 mm maximum diameter) and two required transcranial surgery and radiotherapy. All three affected subjects and ten other unaffected relatives were found to be positive for a germline R271W AIP mutation. Comparison of the single nucleotide polymorphism patterns among this family and two previously reported European FIPA families with the same R271W mutation demonstrated no common ancestry. This kindred exemplifies the aggressive features of pituitary adenomas associated with AIP mutations, while genetic analyses among three R271W FIPA families indicate that R271W represents a mutational hotspot that should be studied further in functional studies.

  7. 40 CFR 80.271 - How can a small refiner obtain an adjustment of its 2004-2007 per-gallon cap standard?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... adjustment of its 2004-2007 per-gallon cap standard? 80.271 Section 80.271 Protection of Environment...-2007 per-gallon cap standard? (a) EPA may in its discretion adjust the small refiner per-gallon cap... that the per-gallon cap creates; (2) The refiner's proposed adjusted per-gallon cap standard and the...

  8. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  9. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  10. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  11. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  12. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the duplicate copy with the documentation required by Block 5 of the form to SDB. The original will be filed in the contract file. (1) If, upon review of the material submitted under 719.271-7(a) above, SDB... documentation required by Block 5 of the form to SDB. In addition to the documentation called for in 719.271-6...

  13. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Bacillus anthracis Infection in BALB/c Mice and Cynomolgus Macaques.

    PubMed

    Grossman, Trudy H; Anderson, Michael S; Drabek, Lindsay; Gooldy, Melanie; Heine, Henry S; Henning, Lisa N; Lin, Winston; Newman, Joseph V; Nevarez, Rene; Siefkas-Patterson, Kaylyn; Radcliff, Anne K; Sutcliffe, Joyce A

    2017-10-01

    The fluorocycline TP-271 was evaluated in mouse and nonhuman primate (NHP) models of inhalational anthrax. BALB/c mice were exposed by nose-only aerosol to Bacillus anthracis Ames spores at a level of 18 to 88 lethal doses sufficient to kill 50% of exposed individuals (LD 50 ). When 21 days of once-daily dosing was initiated at 24 h postchallenge (the postexposure prophylaxis [PEP] study), the rates of survival for the groups treated with TP-271 at 3, 6, 12, and 18 mg/kg of body weight were 90%, 95%, 95%, and 84%, respectively. When 21 days of dosing was initiated at 48 h postchallenge (the treatment [Tx] study), the rates of survival for the groups treated with TP-271 at 6, 12, and 18 mg/kg TP-271 were 100%, 91%, and 81%, respectively. No deaths of TP-271-treated mice occurred during the 39-day posttreatment observation period. In the NHP model, cynomolgus macaques received an average dose of 197 LD 50 of B. anthracis Ames spore equivalents using a head-only inhalation exposure chamber, and once-daily treatment of 1 mg/kg TP-271 lasting for 14 or 21 days was initiated within 3 h of detection of protective antigen (PA) in the blood. No (0/8) animals in the vehicle control-treated group survived, whereas all 8 infected macaques treated for 21 days and 4 of 6 macaques in the 14-day treatment group survived to the end of the study (56 days postchallenge). All survivors developed toxin-neutralizing and anti-PA IgG antibodies, indicating an immunologic response. On the basis of the results obtained with the mouse and NHP models, TP-271 shows promise as a countermeasure for the treatment of inhalational anthrax. Copyright © 2017 American Society for Microbiology.

  14. The Fluorocycline TP-271 Is Efficacious in Models of Aerosolized Bacillus anthracis Infection in BALB/c Mice and Cynomolgus Macaques

    PubMed Central

    Anderson, Michael S.; Drabek, Lindsay; Gooldy, Melanie; Heine, Henry S.; Henning, Lisa N.; Lin, Winston; Newman, Joseph V.; Nevarez, Rene; Siefkas-Patterson, Kaylyn; Radcliff, Anne K.; Sutcliffe, Joyce A.

    2017-01-01

    ABSTRACT The fluorocycline TP-271 was evaluated in mouse and nonhuman primate (NHP) models of inhalational anthrax. BALB/c mice were exposed by nose-only aerosol to Bacillus anthracis Ames spores at a level of 18 to 88 lethal doses sufficient to kill 50% of exposed individuals (LD50). When 21 days of once-daily dosing was initiated at 24 h postchallenge (the postexposure prophylaxis [PEP] study), the rates of survival for the groups treated with TP-271 at 3, 6, 12, and 18 mg/kg of body weight were 90%, 95%, 95%, and 84%, respectively. When 21 days of dosing was initiated at 48 h postchallenge (the treatment [Tx] study), the rates of survival for the groups treated with TP-271 at 6, 12, and 18 mg/kg TP-271 were 100%, 91%, and 81%, respectively. No deaths of TP-271-treated mice occurred during the 39-day posttreatment observation period. In the NHP model, cynomolgus macaques received an average dose of 197 LD50 of B. anthracis Ames spore equivalents using a head-only inhalation exposure chamber, and once-daily treatment of 1 mg/kg TP-271 lasting for 14 or 21 days was initiated within 3 h of detection of protective antigen (PA) in the blood. No (0/8) animals in the vehicle control-treated group survived, whereas all 8 infected macaques treated for 21 days and 4 of 6 macaques in the 14-day treatment group survived to the end of the study (56 days postchallenge). All survivors developed toxin-neutralizing and anti-PA IgG antibodies, indicating an immunologic response. On the basis of the results obtained with the mouse and NHP models, TP-271 shows promise as a countermeasure for the treatment of inhalational anthrax. PMID:28784679

  15. 15 CFR 904.271 - Initial decision.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES Hearing and Appeal Procedures Decision § 904.271 Initial decision. (a) After expiration of the period...

  16. 15 CFR 904.271 - Initial decision.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES Hearing and Appeal Procedures Decision § 904.271 Initial decision. (a) After expiration of the period...

  17. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government..., or because of special skills or knowledge necessary for monitoring the contractor's work. The...

  18. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government..., or because of special skills or knowledge necessary for monitoring the contractor's work. The...

  19. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government..., or because of special skills or knowledge necessary for monitoring the contractor's work. The...

  20. In vitro migration and proliferation ("wound healing") potential of mesenchymal stromal cells generated from human CD271(+) bone marrow mononuclear cells.

    PubMed

    Latifi-Pupovci, Hatixhe; Kuçi, Zyrafete; Wehner, Sibylle; Bönig, Halvard; Lieberz, Ralf; Klingebiel, Thomas; Bader, Peter; Kuçi, Selim

    2015-09-25

    Emerging evidence indicates that mesenchymal stromal cells (MSCs) isolated from different tissue sources may be used in vivo as tissue restorative agents. To date, there is no evidence, however, on migration and proliferation ("wound healing") potential of different subsets of MSCs. The main goal of this study was therefore to compare the in vitro "wound healing" capacity of MSCs generated from positively selected CD271(+) bone marrow mononuclear cells (CD271-MSCs) and MSCs generated by plastic adherence (PA-MSCs). The in vitro model of wound healing (CytoSelect™ 24-Well Wound Healing Assay) was used in order to compare the migration and proliferation potential of CD271-MSCs and PA-MSCs of passage 2 and 4 cultured in presence or absence of growth factors or cytokines. CD271-MSCs of both passages when compared to PA-MSCs demonstrated a significantly higher potential to close the wound 12 and 24 h after initiation of the wound healing assay (P < 0.003 and P < 0.002, respectively). Noteworthy, the migration capacity of PA-MSCs of second passage was significantly improved after stimulation with FGF-2 (P < 0.02), PDGF-BB (P < 0.006), MCP-1 (P < 0.002) and IL-6 (P < 0.03), whereas only TGF-β enhanced significantly migration process of PA-MSCs of P4 12 h after the treatment (P < 0.02). Interestingly, treatment of CD271-MSCs of both passages with growth factors or cytokines did not affect their migratory potential. Our in vitro data provide the first evidence that CD271-MSCs are significantly more potent in "wound healing" than their counterparts PA-MSCs.

  1. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification for...

  2. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification for...

  3. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.6 Complaint... Jackson Blvd., 20th Floor, Chicago, IL 60604-3507. (iv) For Arkansas, Louisiana, New Mexico, Oklahoma, and...

  4. 48 CFR 1516.405-271 - Limitations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1516.405-271 Limitations. (a) No award... satisfactory or excellent. All award fee plans shall disclose to offerors the numerical rating necessary to be...

  5. Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fukuchi, Mikoto; Wanotayan, Rujira; Liu, Sicheng

    2015-06-12

    XRCC4 and DNA Ligase IV (LIG4) cooperate to join two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). However, it is not fully understood how these proteins are localized to the nucleus. Here we created XRCC4{sup K271R} mutant, as Lys271 lies within the putative nuclear localization signal (NLS), and XRCC4{sup K210R} mutant, as Lys210 was reported to undergo SUMOylation, implicated in the nuclear localization of XRCC4. Wild-type and mutated XRCC4 with EGFP tag were introduced into HeLa cell, in which endogenous XRCC4 had been knocked down using siRNA directed to 3′-untranslated region,more » and tested for the nuclear localization function by fluorescence microscopy. XRCC4{sup K271R} was defective in the nuclear localization of itself and LIG4, whereas XRCC4{sup K210R} was competent for the nuclear localization with LIG4. To examine DSB repair function, wild-type and mutated XRCC4 were introduced into XRCC4-deficient M10. M10-XRCC4{sup K271R}, but not M10-XRCC4{sup K210R}, showed significantly reduced surviving fraction after 2 Gy γ-ray irradiation as compared to M10-XRCC4{sup WT}. The number of γ-H2AX foci remaining 2 h after 2 Gy γ-ray irradiation was significantly greater in M10-XRCC4{sup K271R} than in M10-XRCC4{sup WT}, whereas it was only marginally increased in M10-XRCC4{sup K210R} as compared to M10-XRCC4{sup WT}. The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4. - Highlights: • XRCC4{sup K271R} is defective in the nuclear localization of itself and LIG4. • XRCC4{sup K210R} is competent for the nuclear localization of itself and LIG4. • XRCC4{sup K271R} is deficient in DSB repair function. • XRCC4{sup K210R} is mostly normal in DSB repair function.« less

  6. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  7. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  8. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  9. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  10. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  11. Acceptance test procedure bldg. 271-U remote monitoring of project W-059 B-Plant canyon exhaust system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    MCDANIEL, K.S.

    1999-09-01

    The test procedure provides for verifying indications and alarms The test procedure provides for verifying indications and alarms associated with the B Plant Canyon Ventilation System as they are being displayed on a remote monitoring workstation located in building 271-U. The system application software was installed by PLCS Plus under contract from B&W Hanford Company. The application software was installed on an existing operator workstation in building 271U which is owned and operated by Bechtel Hanford Inc.

  12. Dose-Dependent Dual Role of PIT-1 (POU1F1) in Somatolactotroph Cell Proliferation and Apoptosis

    PubMed Central

    Jullien, Nicolas; Roche, Catherine; Brue, Thierry; Figarella-Branger, Dominique; Graillon, Thomas; Barlier, Anne; Herman, Jean-Paul

    2015-01-01

    To test the role of wtPIT-1 (PITWT) or PIT-1 (R271W) (PIT271) in somatolactotroph cells, we established, using inducible lentiviral vectors, sublines of GH4C1 somatotroph cells that allow the blockade of the expression of endogenous PIT-1 and/or the expression of PITWT or PIT271, a dominant negative mutant of PIT-1 responsible for Combined Pituitary Hormone Deficiency in patients. Blocking expression of endogenous PIT-1 induced a marked decrease of cell proliferation. Overexpressing PITWT twofold led also to a dose-dependent decrease of cell proliferation that was accompanied by cell death. Expression of PIT271 induced a strong dose-dependent decrease of cell proliferation accompanied by a very pronounced cell death. These actions of PIT271 are independent of its interaction/competition with endogenous PIT-1, as they were unchanged when expression of endogenous PIT-1 was blocked. All these actions are specific for somatolactotroph cells, and could not be observed in heterologous cells. Cell death induced by PITWT or by PIT271 was accompanied by DNA fragmentation, but was not inhibited by inhibitors of caspases, autophagy or necrosis, suggesting that this cell death is a caspase-independent apoptosis. Altogether, our results indicate that under normal conditions PIT-1 is important for the maintenance of cell proliferation, while when expressed at supra-normal levels it induces cell death. Through this dual action, PIT-1 may play a role in the expansion/regression cycles of pituitary lactotroph population during and after lactation. Our results also demonstrate that the so-called “dominant-negative” action of PIT271 is independent of its competition with PIT-1 or a blockade of the actions of the latter, and are actions specific to this mutant variant of PIT-1. PMID:25822178

  13. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  14. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  15. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  16. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  17. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This insurance shall be for $1,000,000.00 on account of any one accident or occurrence with respect to each...

  18. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) Types of records made available. All records of the Committee that are not available under §§ 271.3 and... requester represents an educational or noncommercial scientific institution, or news media; (3) A statement...

  19. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Types of records made available. All records of the Committee that are not available under §§ 271.3 and... requester represents an educational or noncommercial scientific institution, or news media; (3) A statement...

  20. 78 FR 59752 - Notice of a Record of Decision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ... ROD may be viewed online or during regular business hours at the following locations: 1. Online at www.... Grey may be contacted during business hours at (907) 271-5453 (telephone) and (907) 271-2851 (fax), or...

  1. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... cetacean species (not including mother/calf pairs, unless of species of concern listed in § 216.271(b)(1.... (ii) A single individual or mother/calf pair of any of the following marine mammals of concern: Beaked...

  2. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271...

  3. 14 CFR 271.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.1 Purpose... establishing the fair and reasonable amount of compensation needed to ensure the continuation of essential air...

  4. 40 CFR 271.4 - Consistency.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.4... hazardous waste programs which have received final authorization under this part. (a) Any aspect of the...

  5. 40 CFR 271.4 - Consistency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.4... hazardous waste programs which have received final authorization under this part. (a) Any aspect of the...

  6. 40 CFR 271.4 - Consistency.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.4... hazardous waste programs which have received final authorization under this part. (a) Any aspect of the...

  7. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  8. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  9. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  10. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  11. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land... land treatment program that is designed to ensure that hazardous constituents placed in or on the...

  12. Alzheimer’s Disease in Social Media: Content Analysis of YouTube Videos

    PubMed Central

    Tang, Weizhou; Olscamp, Kate; Friedman, Daniela B

    2017-01-01

    Background Approximately 5.5 million Americans are living with Alzheimer’s disease (AD) in 2017. YouTube is a popular platform for disseminating health information; however, little is known about messages specifically regarding AD that are being communicated through YouTube. Objective This study aims to examine video characteristics, content, speaker characteristics, and mobilizing information (cues to action) of YouTube videos focused on AD. Methods Videos uploaded to YouTube from 2013 to 2015 were searched with the term “Alzheimer’s disease” on April 30th, 2016. Two coders viewed the videos and coded video characteristics (the date when a video was posted, Uniform Resource Locator, video length, audience engagement, format, author), content, speaker characteristics (sex, race, age), and mobilizing information. Descriptive statistics were used to examine video characteristics, content, audience engagement (number of views), speaker appearances in the video, and mobilizing information. Associations between variables were examined using Chi-square and Fisher’s exact tests. Results Among the 271 videos retrieved, 25.5% (69/271) were posted by nonprofit organizations or universities. Informal presentations comprised 25.8% (70/271) of all videos. Although AD symptoms (83/271, 30.6%), causes of AD (80/271, 29.5%), and treatment (76/271, 28.0%) were commonly addressed, quality of life of people with AD (34/271, 12.5%) had more views than those more commonly-covered content areas. Most videos featured white speakers (168/187, 89.8%) who were adults aged 20 years to their early 60s (164/187, 87.7%). Only 36.9% (100/271) of videos included mobilizing information. Videos about AD symptoms were significantly less likely to include mobilizing information compared to videos without AD symptoms (23/83, 27.7% vs 77/188, 41.0% respectively; P=.03). Conclusions This study contributes new knowledge regarding AD messages delivered through YouTube. Findings of the current study highlight a potential gap between available information and viewers’ interests. YouTube videos on AD could be beneficial if the messages delivered meet users’ needs and provide mobilizing information for further resources. Study findings will be useful to government agencies, researchers, nonprofit organizations that promote information about AD, and those responsible for social media to provide useful and accurate health information for the public. PMID:29051137

  13. Alzheimer's Disease in Social Media: Content Analysis of YouTube Videos.

    PubMed

    Tang, Weizhou; Olscamp, Kate; Choi, Seul Ki; Friedman, Daniela B

    2017-10-19

    Approximately 5.5 million Americans are living with Alzheimer's disease (AD) in 2017. YouTube is a popular platform for disseminating health information; however, little is known about messages specifically regarding AD that are being communicated through YouTube. This study aims to examine video characteristics, content, speaker characteristics, and mobilizing information (cues to action) of YouTube videos focused on AD. Videos uploaded to YouTube from 2013 to 2015 were searched with the term "Alzheimer's disease" on April 30th, 2016. Two coders viewed the videos and coded video characteristics (the date when a video was posted, Uniform Resource Locator, video length, audience engagement, format, author), content, speaker characteristics (sex, race, age), and mobilizing information. Descriptive statistics were used to examine video characteristics, content, audience engagement (number of views), speaker appearances in the video, and mobilizing information. Associations between variables were examined using Chi-square and Fisher's exact tests. Among the 271 videos retrieved, 25.5% (69/271) were posted by nonprofit organizations or universities. Informal presentations comprised 25.8% (70/271) of all videos. Although AD symptoms (83/271, 30.6%), causes of AD (80/271, 29.5%), and treatment (76/271, 28.0%) were commonly addressed, quality of life of people with AD (34/271, 12.5%) had more views than those more commonly-covered content areas. Most videos featured white speakers (168/187, 89.8%) who were adults aged 20 years to their early 60s (164/187, 87.7%). Only 36.9% (100/271) of videos included mobilizing information. Videos about AD symptoms were significantly less likely to include mobilizing information compared to videos without AD symptoms (23/83, 27.7% vs 77/188, 41.0% respectively; P=.03). This study contributes new knowledge regarding AD messages delivered through YouTube. Findings of the current study highlight a potential gap between available information and viewers' interests. YouTube videos on AD could be beneficial if the messages delivered meet users' needs and provide mobilizing information for further resources. Study findings will be useful to government agencies, researchers, nonprofit organizations that promote information about AD, and those responsible for social media to provide useful and accurate health information for the public. ©Weizhou Tang, Kate Olscamp, Seul Ki Choi, Daniela B Friedman. Originally published in the Interactive Journal of Medical Research (http://www.i-jmr.org/), 19.10.2017.

  14. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.5 Carrier revenues. (a) The projected passenger revenue for a carrier providing essential air service at an eligible...

  15. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.4 Carrier costs. (a) The reasonable costs projected for a carrier providing essential air service at an eligible...

  16. 14 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.2 Definitions... compensation under Subchapter II of Chapter 417 of the Statute. Essential air service is that air...

  17. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.6 Profit element. The reasonable return for a carrier for providing essential air service at an eligible place...

  18. 75 FR 38540 - Notice of Filing of Plats of Survey, Alaska

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ...: Stephen B. Hamrick, 907-271-5481, fax 907-271-4549, e-mail [email protected] . SUPPLEMENTARY INFORMATION.... Stephen B. Hamrick, Chief Cadastral Surveyor. [FR Doc. 2010-16220 Filed 7-1-10; 8:45 am] BILLING CODE 4310...

  19. 75 FR 53333 - Notice of Filing of Plats of Survey, Alaska

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... CONTACT: Stephen B. Hamrick, 907-271-5481, fax 907-271-4549, e-mail [email protected] . SUPPLEMENTARY... guarantee that we will be able to do so. Authority: 43 U.S.C. 3; 53. Stephen B. Hamrick, Chief Cadastral...

  20. 48 CFR 719.271-5 - Cognizant technical officers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-5 Cognizant technical officers. Since the procurement process starts with the establishment of a requirement, the actions of the cognizant technical officers can affect the opportunity of small business to participate equitably...

  1. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  2. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  3. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  4. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  5. 40 CFR 424.35 - Standards of performance for new sources.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

  6. Mapping selected general literature of international nursing.

    PubMed

    Shams, Marie-Lise Antoun; Dixon, Lana S

    2007-01-01

    This study, part of a wider project to map the literature of nursing, identifies core journals cited in non-US nursing journals and determines the extent of their coverage by indexing services. Four general English-language journals were analyzed for format types and publication dates. Core titles were identified and nine bibliographic databases were scanned for indexing coverage. Findings show that 57.5% (13,391/23,271) of the cited references from the 4 core journals were to journal articles, 27.8% (6,471/23,271) to books, 9.5% (2,208/23,271) to government documents, 4.9% (1,131/23,271) to miscellaneous sources, and less than 1% (70/23,271) to Internet resources. Eleven journals produced one-third of the citations; the next third included 146 journals, followed by a dispersion of 1,622 titles. PubMed received the best database coverage scores, followed by CINAHL and Science Citation Index. None of the databases provided complete coverage of all 11 core titles. The four source journals contain a diverse group of cited references. The currency of citations to government documents makes these journals a good source for regulatory and legislative awareness. Nurses consult nursing and biomedical journals and must search both nursing and biomedical databases to cover the literature.

  7. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period... place essential air service level; or (5) The uncertainties of the market or other circumstances warrant...

  8. 14 CFR 271.3 - Carrier subsidy need.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.3 Carrier subsidy need. In establishing the subsidy for an air carrier providing essential air service at an...

  9. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TREASURY LIQUORS BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as...

  10. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TREASURY LIQUORS BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and operate a pilot brewing plant off the brewery premises for research, analytical, experimental, or developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as...

  11. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System (ISMLS) § 171.271 Installation requirements. (a) The ISMLS facility must be permanent in nature, located... codes, FCC licensing requirements, and paragraphs (a) and (c) of § 171.261. (b) The ISMLS facility must...

  12. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System (ISMLS) § 171.271 Installation requirements. (a) The ISMLS facility must be permanent in nature, located... codes, FCC licensing requirements, and paragraphs (a) and (c) of § 171.261. (b) The ISMLS facility must...

  13. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System (ISMLS) § 171.271 Installation requirements. (a) The ISMLS facility must be permanent in nature, located... codes, FCC licensing requirements, and paragraphs (a) and (c) of § 171.261. (b) The ISMLS facility must...

  14. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor (GTM...

  15. Isolation and characterisation of mesenchymal stem/stromal cells in the ovine endometrium.

    PubMed

    Letouzey, Vincent; Tan, Ker Sin; Deane, James A; Ulrich, Daniela; Gurung, Shanti; Ong, Y Rue; Gargett, Caroline E

    2015-01-01

    Mesenchymal stem/stromal cells (MSC) were recently discovered in the human endometrium. These cells possess key stem cell properties and show promising results in small animal models when used for preclinical tissue engineering studies. A small number of surface markers have been identified that enrich for MSC from bone marrow and human endometrium, including the Sushi Domain-containing 2 (SUSD2; W5C5) and CD271 markers. In preparation for developing a large animal preclinical model for urological and gynecological tissue engineering applications we aimed to identify and characterise MSC in ovine endometrium and determine surface markers to enable their prospective isolation. Ovine endometrium was obtained from hysterectomised ewes following progesterone synchronisation, dissociated into single cell suspensions and tested for MSC surface markers and key stem cell properties. Purified stromal cells were obtained by flow cytometry sorting with CD49f and CD45 to remove epithelial cells and leukocytes respectively, and MSC properties investigated. There was a small population CD271+ stromal cells (4.5 ± 2.3%) in the ovine endometrium. Double labelling with CD271 and CD49f showed that the sorted CD271+CD49f- stromal cell population possessed significantly higher cloning efficiency, serial cloning capacity and a qualitative increased ability to differentiate into 4 mesodermal lineages (adipocytic, smooth muscle, chondrocytic and osteoblastic) than CD271-CD49f- cells. Immunolabelling studies identified an adventitial perivascular location for ovine endometrial CD271+ cells. This is the first study to characterise MSC in the ovine endometrium and identify a surface marker profile identifying their location and enabling their prospective isolation. This knowledge will allow future preclinical studies with a large animal model that is well established for pelvic organ prolapse research.

  16. 27 CFR 19.271 - Construction of buildings

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Construction of buildings..., DEPARTMENT OF THE TREASURY LIQUORS DISTILLED SPIRITS PLANTS Construction, Equipment and Security § 19.271 Construction of buildings Buildings in which spirits, denatured spirits, articles, or wines are produced...

  17. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... from the Publications Services of the Federal Reserve Board, 20th Street and Constitution Avenue, N.W... Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the...

  18. 12 CFR 271.3 - Published information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... from the Publications Services of the Federal Reserve Board, 20th Street and Constitution Avenue, N.W... Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.3 Published information. (a) Federal Register. The Committee publishes in the...

  19. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Committee includes rules, statements, decisions, minutes, memoranda, letters, reports, transcripts, accounts... INFORMATION § 271.2 Definitions. (a) Board means the Board of Governors of the Federal Reserve System... from or on behalf of one who seeks information for a use or purpose that furthers the commercial, trade...

  20. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  1. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  2. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  3. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... contracts to SDB for screening (see 719.271-6); (h) Taking action to assure that unnecessary qualifications... determinations, the contracting officer shall consider the recommendations, if any, of SDB together with the... small minority business enterprises, to SDB for information and advice; (k) Promoting increased...

  4. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review Form...

  5. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission may...

  6. Photocopy of drawing located at National Archives, San Bruno, California ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of drawing located at National Archives, San Bruno, California (Navy 271-A-17). Minsy building 271 record 1st floor plan; N.D. - Mare Island Naval Shipyard, Mechanics Shop, Waterfront Avenue, west side between A Street & Third Street, Vallejo, Solano County, CA

  7. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  8. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  9. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  10. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  11. 7 CFR 1924.271 - Processing applications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS CONSTRUCTION AND REPAIR Complaints and Compensation for Construction Defects § 1924.271 Processing applications. An application for compensation for construction defects shall be submitted by the claimant to Fm... entirety. All structural defects and claims for which compensation is sought will be listed. Borrowers will...

  12. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  13. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  14. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  15. 48 CFR 1352.271-79 - Liability and insurance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-79... against all suits, actions, claims, costs or demands (including without limitation, suits, actions, claims.... Such indemnity shall include, without limitation, suits, actions, claims, costs or demands of any kind...

  16. Eielson AFB, Alaska. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    DTIC Science & Technology

    1987-12-01

    26/ 609 111 301 13/ 369 13O 271 1 301 281 60 j I :7T 239 259 9/ 309 1lt 271 41 299 29 309 26/ 231 26/ 311 26/ 229 261 281 29O 629 271 61 271 6 ?I I ISO ... 27001 90.9 93. 9 98.8 95.2 96.8 96.9 96.1 96.3 96.6 96.6 96.6 96.6 96.6 96.6 96.6 96.6 b E 1803o 91.1 94.2 95.1 95.8 96.6 96.6 96.8 97.0 97.2 97.2...91.9 91.6 91.6 91.6 91.9 91.9 91.9 92.0 92.0 92.0 92.0 2.0 92.0 92.0 6E 27001 67.0 92.0 92.6 92.9 93.3 93.3 93.3 93.3 93.3 93.0 93.9 91.4 93.0 93.4

  17. Fluorocycline TP-271 Is Potent against Complicated Community-Acquired Bacterial Pneumonia Pathogens

    PubMed Central

    Fyfe, Corey; O’Brien, William; Hackel, Meredith; Minyard, Mary Beth; Waites, Ken B.; Dubois, Jacques; Murphy, Timothy M.; Slee, Andrew M.; Weiss, William J.; Sutcliffe, Joyce A.

    2017-01-01

    ABSTRACT TP-271 is a novel, fully synthetic fluorocycline antibiotic in clinical development for the treatment of respiratory infections caused by susceptible and multidrug-resistant pathogens. TP-271 was active in MIC assays against key community respiratory Gram-positive and Gram-negative pathogens, including Streptococcus pneumoniae (MIC90 = 0.03 µg/ml), methicillin-sensitive Staphylococcus aureus (MSSA; MIC90 = 0.25 µg/ml), methicillin-resistant S. aureus (MRSA; MIC90 = 0.12 µg/ml), Streptococcus pyogenes (MIC90 = 0.03 µg/ml), Haemophilus influenzae (MIC90 = 0.12 µg/ml), and Moraxella catarrhalis (MIC90 ≤0.016 µg/ml). TP-271 showed activity (MIC90 = 0.12 µg/ml) against community-acquired MRSA expressing Panton-Valentine leukocidin (PVL). MIC90 values against Mycoplasma pneumoniae, Legionella pneumophila, and Chlamydia pneumoniae were 0.004, 1, and 4 µg/ml, respectively. TP-271 was efficacious in neutropenic and immunocompetent animal pneumonia models, generally showing, compared to the burden at the start of dosing, ~2 to 5 log10 CFU reductions against MRSA, S. pneumoniae, and H. influenzae infections when given intravenously (i.v.) and ~1 to 4 log10 CFU reductions when given orally (p.o.). TP-271 was potent against key community-acquired bacterial pneumonia (CABP) pathogens and was minimally affected, or unaffected, by tetracycline-specific resistance mechanisms and fluoroquinolone or macrolide drug resistance phenotypes. IMPORTANCE Rising resistance rates for macrolides, fluoroquinolones, and β-lactams in the most common pathogens associated with community-acquired bacterial pneumonia (CABP) are of concern, especially for cases of moderate to severe infections in vulnerable populations such as the very young and the elderly. New antibiotics that are active against multidrug-resistant Streptococcus pneumoniae and Staphylococcus aureus are needed for use in the empirical treatment of the most severe forms of this disease. TP-271 is a promising new fluorocycline antibiotic demonstrating in vitro potency and nonclinical efficacy by intravenous and oral administration against the major pathogens associated with moderate to severe CABP. PMID:28251179

  18. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a...), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining the...

  19. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE FISHERIES OF THE CARIBBEAN, GULF OF MEXICO, AND SOUTH ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a...), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining the...

  20. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  1. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  2. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  3. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  4. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Helicopter hospital emergency medical....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... certificate holder may assign a helicopter flight crewmember, and no flight crewmember may accept an...

  5. 34 CFR 271.10 - What types of projects may be funded?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... members, and other elementary or secondary school personnel to deal effectively with special educational... 271.10 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND... Secretary awards grants to SEAs for projects offering technical assistance (including training) to school...

  6. 34 CFR 271.10 - What types of projects may be funded?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... members, and other elementary or secondary school personnel to deal effectively with special educational... 271.10 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND... Secretary awards grants to SEAs for projects offering technical assistance (including training) to school...

  7. 47 CFR 0.271 - Authority delegated.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... of Strategic Planning and Policy Analysis § 0.271 Authority delegated. (a) Insofar as authority is... status, the Chief, Office of Strategic Planning and Policy Analysis is delegated authority to deny... Strategic Planning and Policy Analysis has primary authority. (b) [Reserved] [45 FR 10347, Feb. 15, 1980, as...

  8. Prothrombin activation on the activated platelet surface optimizes expression of procoagulant activity

    PubMed Central

    Wood, Jeremy P.; Silveira, Jay R.; Maille, Nicole M.; Haynes, Laura M.

    2011-01-01

    Effective hemostasis relies on the timely formation of α-thrombin via prothrombinase, a Ca2+-dependent complex of factors Va and Xa assembled on the activated platelet surface, which cleaves prothrombin at Arg271 and Arg320. Whereas initial cleavage at Arg271 generates the inactive intermediate prethrombin-2, initial cleavage at Arg320 generates the enzymatically active intermediate meizothrombin. To determine which of these intermediates is formed when prothrombin is processed on the activated platelet surface, the cleavage of prothrombin, and prothrombin mutants lacking either one of the cleavage sites, was monitored on the surface of either thrombin- or collagen-activated platelets. Regardless of the agonist used, prothrombin was initially cleaved at Arg271 generating prethrombin-2, with α-thrombin formation quickly after via cleavage at Arg320. The pathway used was independent of the source of factor Va (plasma- or platelet-derived) and was unaffected by soluble components of the platelet releasate. When both cleavage sites are presented within the same substrate molecule, Arg271 effectively competes against Arg320 (with an apparent IC50 = 0.3μM), such that more than 90% to 95% of the initial cleavage occurs at Arg271. We hypothesize that use of the prethrombin-2 pathway serves to optimize the procoagulant activity expressed by activated platelets, by limiting the anticoagulant functions of the alternate intermediate, meizothrombin. PMID:21131592

  9. Prothrombin activation on the activated platelet surface optimizes expression of procoagulant activity.

    PubMed

    Wood, Jeremy P; Silveira, Jay R; Maille, Nicole M; Haynes, Laura M; Tracy, Paula B

    2011-02-03

    Effective hemostasis relies on the timely formation of α-thrombin via prothrombinase, a Ca(2+)-dependent complex of factors Va and Xa assembled on the activated platelet surface, which cleaves prothrombin at Arg271 and Arg320. Whereas initial cleavage at Arg271 generates the inactive intermediate prethrombin-2, initial cleavage at Arg320 generates the enzymatically active intermediate meizothrombin. To determine which of these intermediates is formed when prothrombin is processed on the activated platelet surface, the cleavage of prothrombin, and prothrombin mutants lacking either one of the cleavage sites, was monitored on the surface of either thrombin- or collagen-activated platelets. Regardless of the agonist used, prothrombin was initially cleaved at Arg271 generating prethrombin-2, with α-thrombin formation quickly after via cleavage at Arg320. The pathway used was independent of the source of factor Va (plasma- or platelet-derived) and was unaffected by soluble components of the platelet releasate. When both cleavage sites are presented within the same substrate molecule, Arg271 effectively competes against Arg320 (with an apparent IC(50) = 0.3μM), such that more than 90% to 95% of the initial cleavage occurs at Arg271. We hypothesize that use of the prethrombin-2 pathway serves to optimize the procoagulant activity expressed by activated platelets, by limiting the anticoagulant functions of the alternate intermediate, meizothrombin.

  10. Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum-plasma membrane contact sites.

    PubMed

    Siao, Wei; Wang, Pengwei; Voigt, Boris; Hussey, Patrick J; Baluska, Frantisek

    2016-11-01

    Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulum-plasma membrane (ER-PM) contact sites in leaf and root cells. The ER-PM localization of Arabidopsis SYT1 resembles that of the extended synaptotagmins (E-SYTs) in animal cells. In mammals, E-SYTs have been shown to regulate calcium signaling, lipid transfer, and endocytosis. Arabidopsis SYT1 was reported to be essential for maintaining cell integrity and virus movement. This study provides detailed insight into the subcellular localization of SYT1 and VAP27-1, another ER-PM-tethering protein. SYT1 and VAP27-1 were shown to be localized on distinct ER-PM contact sites. The VAP27-1-enriched ER-PM contact sites (V-EPCSs) were always in contact with the SYT1-enriched ER-PM contact sites (S-EPCSs). The V-EPCSs still existed in the leaf epidermal cells of the SYT1 null mutant; however, they were less stable than those in the wild type. The polygonal networks of cortical ER disassembled and the mobility of VAP27-1 protein on the ER-PM contact sites increased in leaf cells of the SYT1 null mutant. These results suggest that SYT1 is responsible for stabilizing the ER network and V-EPCSs. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  11. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c...

  12. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Requirements for generators of... for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State program must cover all generators covered by 40 CFR part 262. States must require new generators to...

  13. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  14. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  15. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  16. 24 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... FORECLOSURE OF MULTIFAMILY AND SINGLE FAMILY MORTGAGES Nonjudicial Foreclosure of Multifamily Mortgages § 27.1... Multifamily Mortgage Foreclosure Act of 1981 (the Act) (12 U.S.C. 3701-3717), that clarify, or are in addition... multifamily mortgages. Under a delegation of authority published on February 5, 1982 (47 FR 5468), the...

  17. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... fostering public information in the prevention of forest fires. All authorities and licenses granted under... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Power to revoke. 271.7...

  18. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for used oil management... Final Authorization § 271.26 Requirements for used oil management. The State shall have standards for used oil management which are equivalent to 40 CFR part 279. These standards shall include: (a...

  19. 76 FR 8626 - Revision of Class E Airspace; Shungnak, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ...-1104; Airspace Docket No. 10-AAL-19] Revision of Class E Airspace; Shungnak, AK AGENCY: Federal... Shungnak, AK, to accommodate amended Standard Instrument Approach Procedures (SIAPs) at the Shungnak..., Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850; e- mail...

  20. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... fostering public information in the prevention of forest fires. All authorities and licenses granted under... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Power to revoke. 271.7...

  1. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Commercial license. 271.4...

  2. 76 FR 8627 - Revision of Class E Airspace; Platinum, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ...-1105; Airspace Docket No. 10-AAL-20] Revision of Class E Airspace; Platinum, AK AGENCY: Federal... Platinum, AK, to accommodate the addition of a Standard Instrument Approach Procedure (SIAP), at the... 7th Avenue, Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850; e...

  3. 75 FR 62458 - Revision of Class E Airspace; Tanana, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-12

    ...-0588 Airspace Docket No. 10-AAL-16] Revision of Class E Airspace; Tanana, AK AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action revises Class E airspace at Tanana, AK. The... West 7th Avenue, Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850...

  4. 75 FR 12680 - Revision of Class E Airspace; Scammon Bay, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-17

    ...-1038; Airspace Docket No. 09-AAL-19] Revision of Class E Airspace; Scammon Bay, AK AGENCY: Federal... Scammon Bay, AK, to accommodate new Area Navigation (RNAV) Standard Instrument Approach Procedures (SIAPs..., 222 West 7th Avenue, Box 14, Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271...

  5. 75 FR 32272 - Revision of Class E Airspace; Kaltag, AK

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ...-0082; Airspace Docket No. 10-AAL-4] Revision of Class E Airspace; Kaltag, AK AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action revises Class E airspace at Kaltag, AK, to..., Anchorage, AK 99513-7587; telephone number (907) 271-5898; fax: (907) 271-2850; e-mail: [email protected

  6. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Commercial license. 271.4...

  7. 26. BUILDING NO. 271I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. BUILDING NO. 271-I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER OF BUILDING SHOWING DOORS TO MIXING ROOM NO. 4. HAND CRANK VISIBLE AT RIGHT ROTATED SMALL POTS (CONTAINING LEAD AZIDE) IN MIXING ROOMS. - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  8. Corrective Action Plan for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems, Nevada Test Site, Nevada

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    R. B. Jackson

    2003-05-01

    The Areas 25, 26 and 27 Septic Systems are in the Federal Facility Agreement and Consent Order (FFACO) of 1996 as Corrective Action Unit (CAU) 271. This Corrective Action Plan (CAP) provides selected corrective action alternatives and proposes the closure methodology for CAU 271. CAU 271 is located on the Nevada Test Site (NTS) approximately 105 kilometers (65 miles) northwest of Las Vegas, Nevada, and consists of the following 15 Corrective Action Sites (CAS): CAS 25-04-1, Septic System; CAS 25-04-03, Septic System; CAS25-04-04, Septic System; CAS 25-04-08, Septic System; CAS 25-04-09, Septic System; CAS 25-04-10, Septic System; CAS 25-04-11, Septicmore » System; CAS 26-03-01, Contaminated Water Reservoir; CAS 26-04-1, Septic System; CAS 26-04-02, Septic System; CAS 26-05-01, Radioactive Leachfield; CAS-26-05-03, Septic System; CAS 26-05-04, Septic System; CAS 26-05-05, Septic System; and CAS 27-05-02, Leachfield.« less

  9. Evaluation of acetone vapors toxicity on Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae) eggs.

    PubMed

    Pourmirza, Ali Asghr; Nasab, Fershteh Sadeghi; Zadeh, Abas Hossein

    2007-08-01

    The efficacy of acetone vapors against carefully aged eggs of Plodia interpunctella (Hubner) at 17+/-1 and 27+/-1 degrees C at different dosage levels of acetone over various exposure times was determined. Acetone was found to be toxic to Indian meal moth eggs. Considerable variation in the susceptibility of different age groups of eggs was apparent in the fiducial limits of the LD50 values. An inverse relationship between LD50 values and exposure times was observed in age groups of tested eggs. At 27+/-1 degrees C and 24 h exposure period, eggs aged 1-2 day-old were more tolerant to acetone than other age groups, followed by 0-1 day-old, 2-3 day-old and 3-4 day-old eggs. A similar pattern of susceptibility of eggs was observed at 72 h exposure. In all bioassays, eggs exposed to higher dosages of acetone developed at smaller rate. This was significant for the eggs, which were exposed to the highest dosage for 24 h. Increasing the temperature from 17+/-1 to 27+/-1 degrees C greatly increased the efficacy of acetone. At 27+/-1 degrees C eggs of P. interpunctella were killed by less than one-third of the dosage required for control at 17+/-1 degrees C. Acetone achieved 50% mortality with a dosage of 82.76 mg L(-1) in 1-2 day-old eggs at 27+/-1 degrees C. At this temperature hatching was retarded and greatly diminished when eggs aged 1-2 day-old were exposed to 80 mg L(-1) of acetone for the 24 h exposure period. There was no evidence of a hatch delay longer than the time spent under vapors for eggs exposed at 17+/-1 or 27+/-1 degrees C, indicating that some development must have occurred under fumigation.

  10. 30 CFR 250.271 - For what reasons will MMS disapprove the DPP or DOCD?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Decision Process for the Dpp Or Docd § 250.271 For what reasons will MMS disapprove the DPP or DOCD? The...), implementing regulations, or other applicable Federal laws. (b) No consistency concurrence. (1) An affected State has not yet issued a final decision on your coastal zone consistency certification (see 15 CFR 930...

  11. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2013-10-01 2013-10-01 false Status conference. 1.733 Section 1.733...

  12. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2010-10-01 2010-10-01 false Status conference. 1.733 Section 1.733...

  13. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2014-10-01 2014-10-01 false Status conference. 1.733 Section 1.733...

  14. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2011-10-01 2011-10-01 false Status conference. 1.733 Section 1.733...

  15. 47 CFR 1.733 - Status conference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., and key legal issues; and (vi) In a 47 U.S.C. 271(d)(6)(B) proceeding, whether or not the parties agree to waive the 47 U.S.C. 271(d)(6)(B) 90-day resolution deadline. (2) Subject to paragraph (i) of... 47 Telecommunication 1 2012-10-01 2012-10-01 false Status conference. 1.733 Section 1.733...

  16. Protein Engineering and Homologous Expression of Serratia marcescens Lipase for Efficient Synthesis of a Pharmaceutically Relevant Chiral Epoxyester.

    PubMed

    Chen, Ke-Cai; Zheng, Ming-Min; Pan, Jiang; Li, Chun-Xiu; Xu, Jian-He

    2017-10-01

    The lipase isolated from Serratia marcescens (LipA) is a useful biocatalyst for kinetic resolution of a pharmaceutically relevant epoxyester, (±)-3-(4'-methoxyphenyl) glycidic acid methyl ester [(±)-MPGM], to afford optically pure (-)-MPGM, a key intermediate for the synthesis of diltiazem hydrochloride. Two mutants, LipA L315S and LipA S271F , were identified from the combinatorial saturation mutation library of 14 amino acid residues lining the substrate-binding pocket. LipA L315S , LipA S271F , and their combination LipA L315S/S271F showed 2.6-, 2.2-, and 4.6-fold improvements in their specific activities towards para-nitrophenyl butyrate (pNPB), respectively. Among these positive mutants, LipA S271F displayed a 3.5-fold higher specific activity towards the pharmaco substrate (±)-MPGM. Kinetic study showed that the improvement in catalytic efficiency of LipA S271F against (±)-MPGM was mainly resulted from the enhanced affinity between substrate and enzyme, as indicated by the decrease of K m . Furthermore, to address the insoluble expression issue in Escherichia coli, the homologous expression of LipA gene in S. marcescens was achieved by introducing it into an expression vector pUC18, resulting in ca. 20-fold higher lipase production. The significantly improved volumeric production and specific activity of S. marcescens lipase make it very attractive as a new-generation biocatalyst for more efficient and economical manufacturing of (-)-MPGM.

  17. Immunohistochemical CD271 expression correlates with melanoma progress in a case-control study.

    PubMed

    Nielsen, Patricia Switten; Riber-Hansen, Rikke; Steiniche, Torben

    2018-06-01

    Putative cancer stem cell (CSC) markers have arisen from melanoma mouse and in vitro models, but their expression in paraffin embedded patient samples relative to clinical outcome remains largely unexplored. Rather than cells of the tumour bulk, conceivably, CSC drive tumour progression. Accordingly, complete eradication may prevent melanoma relapse. Because elevated tumour-cell proliferation is an established indicator of aggressive disease, this study aimed to investigate the correlation between melanoma recurrence and proliferation of putative CSC that express CD271, CD166, or CD20. Additionally, the expression of these markers was studied in naevi, melanomas, and their recurrence. In melanoma patients, 30 with relapse (cases) and 30 without (controls) were matched for tumour thickness, ulceration, Clark level, subtype, site, gender, and age. One paraffin-embedded section of the patients' primary melanoma (n = 60), relapse (n = 21), and naevus (n = 17) were immunohistochemically double-stained for Ki-67/MART1 and single-stained for CD271, CD166, and CD20. Their whole slide images were aligned as virtual quadruple stains. Image analysis established proliferation indices of each putative stem cell marker and the tumour bulk in addition to the markers' percentage level in tumour areas and the epidermis. In cases vs controls, median dermal proliferation indices (no./mm 2 ) were 211 vs 103 (p = 0.04) for CD271, 512 vs 227 (p = 0.3) for CD166, 184 vs 97 (p = 0.3) for CD20, and 95 vs 103 (p = 0.6) for the tumour bulk. Of additional interest, epidermal CD271 + keratinocytes totalled 8.8% in naevi and 0.98% in melanomas (p = 0.0007). Even though differences between naevi and melanomas also were observed for CD166 in both the epidermis (p = 0.002) and dermis (p = 0.006), they were visually less apparent. CD20 + MART1 + cells were absent in half of the melanomas, and all naevi and relapses. In conclusion, high levels of CD271 + Ki-67 + MART1 + cells were linked to melanoma relapse as opposed to common Ki-67 indices in this particular case-control study. With further investigation, such cells could be potential targets of therapy. Especially, loss of epidermal CD271 + keratinocytes seemed necessary for melanoma development; hence, identification may serve as a diagnostic tool with additional research. Copyright © 2018 Royal College of Pathologists of Australasia. Published by Elsevier B.V. All rights reserved.

  18. An Overview of Production Systems

    DTIC Science & Technology

    1975-10-01

    DISTRIBUTED BY: Matonal Tochnica! Infonu srice U. S. DEPARTMENT OF COMMERCE 028143 Stanford Artificil Inteligence Laboratory October 1975 Memo AIM-271...ORGANIZATION NAMEL AND ADDRESS 18. PROGRAM ELEMENT. PROJECT. TASK Artificial Intelligence Laboratory AE OKUI UBR Stanford University ARPA Order 249...014-64011I j SEC-jRITY CLASSIFICATION OF THIS PAGE (When, Data bHISP011 A Stanford Artificial ktteligncs Laboratory October 1975 Memo AIM-271 Computer

  19. Decision Aiding and Coordination in Decision-Making Organizations.

    DTIC Science & Technology

    1988-01-01

    30111 211100 30131 281 150 90 131 231 100 40 9 151 241 90 30151 241 90 30181 331 150 90 161 261 100 40 10 181 271 90 30 181 271 90 30231 381 150 90 181...check mark is present, the delayed execution will take place. I’, Hm ~One: function: Used to specify that the execution is implemented one firing at a

  20. Carboxylesterase-mediated insecticide resistance: Quantitative increase induces broader metabolic resistance than qualitative change.

    PubMed

    Cui, Feng; Li, Mei-Xia; Chang, Hai-Jing; Mao, Yun; Zhang, Han-Ying; Lu, Li-Xia; Yan, Shuai-Guo; Lang, Ming-Lin; Liu, Li; Qiao, Chuan-Ling

    2015-06-01

    Carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (OP) insecticides. Carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (G/A151D) or from tryptophan to leucine at the 271 site (W271L), following the numbering of Drosophila melanogaster AChE. Qualitative mechanism has been observed in few species. However, whether this carboxylesterase mutation mechanism is prevalent in insects remains unclear. In this study, wild-type, G/A151D and W271L mutant carboxylesterases from Culex pipiens and Aphis gossypii were subjected to germline transformation and then transferred to D. melanogaster. These germlines were ubiquitously expressed as induced by tub-Gal4. In carboxylesterase activity assay, the introduced mutant carboxylesterase did not enhance the overall carboxylesterase activity of flies. This result indicated that G/A151D or W271L mutation disrupted the original activities of the enzyme. Less than 1.5-fold OP resistance was only observed in flies expressing A. gossypii mutant carboxylesterases compared with those expressing A. gossypii wild-type carboxylesterase. However, transgenic flies universally showed low resistance to OP insecticides compared with non-transgenic flies. The flies expressing A. gossypii W271L mutant esterase exhibited 1.5-fold resistance to deltamethrin, a pyrethroid insecticide compared with non-transgenic flies. The present transgenic Drosophila system potentially showed that a quantitative increase in carboxylesterases induced broader resistance of insects to insecticides than a qualitative change. Copyright © 2014 Elsevier Inc. All rights reserved.

  1. Development of a Procurement Task Classification Scheme.

    DTIC Science & Technology

    1987-12-01

    Office of Sci- entific Research, Arlington, Virginia, January 1970. Tornow , Walter W . and Pinto, Patrick R. "The Development of a Man- agerial Job...classification. [Ref. 4:271 -. 20 6 %° w Numerical taxonomy proponents hold [Ref. 4:271, ... that the relationships of contiguity and similarity should be...solving. 22 W i * These primitive categories are based on a sorting of learning pro- cesses into classes that have obvious differences at the

  2. Identification of essential histidine residues in the active site of Escherichia coli xylose (glucose) isomerase.

    PubMed Central

    Batt, C A; Jamieson, A C; Vandeyar, M A

    1990-01-01

    Two conserved histidine residues (His-101 and His-271) appear to be essential components in the active site of the enzyme xylose (glucose) isomerase (EC 5.3.1.5). These amino acid residues were targeted for mutagenesis on the basis of sequence homology among xylose isomerases isolated from Escherichia coli, Bacillus subtilis, Ampullariella sp. strain 3876, and Streptomyces violaceus-niger. Each residue was selectively replaced by site-directed mutagenesis and shown to be essential for activity. No measurable activity was observed for any mutations replacing either His-101 or His-271. Circular dichroism measurements revealed no significant change in the overall conformation of the mutant enzymes, and all formed dimers similar to the wild-type enzyme. Mutations at His-271 could be distinguished from those at His-101, since the former resulted in a thermolabile protein whereas no significant change in heat stability was observed for the latter. Based upon these results and structural data recently reported, we speculate that His-101 is the catalytic base mediating the reaction. Replacement of His-271 may render the enzyme thermolabile, since this residue appears to be a ligand for one of the metal ions in the active site of the enzyme. Images PMID:2405386

  3. Electronic State Decomposition of Energetic Materials and Model Systems

    DTIC Science & Technology

    2010-11-17

    Nitromethane at 226 nm and 271 nm at both Nanosecond and Femtosecond Temporal Scales," J. Phys. Chem. A 113, 85 (2009). Y. Q. Guo, A. Bhattacharya and E...less "energetic". 8. Photodissociation Dynamics of Nitromethane at 226 and 271 nm at Both Nanosecond and Femtosecond Time Scales Photodissociation...of nitromethane has been investigated for decades both theoretically and experimentally; however, as a whole picture, the dissociation dynamics for

  4. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 9 2012-10-01 2012-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  5. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 9 2013-10-01 2013-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  6. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 9 2014-10-01 2014-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  7. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  8. 46 CFR Exhibit No. 1 to Subpart Q... - Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 9 2011-10-01 2011-10-01 false Application for Refund or Waiver of Freight Charges Due to Tariff or Quoting Error No. Exhibit No. 1 to Subpart Q [§ 502.271(d)] of Part 502 Shipping... or Waiver of Freight Charges Pt. 502, Subpt. Q, Exh. 1 Exhibit No. 1 to Subpart Q [§ 502.271(d)] of...

  9. 27. BUILDING NO. 271I, INTERIOR OF LEAD AZIDE MIXING ROOM. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. BUILDING NO. 271-I, INTERIOR OF LEAD AZIDE MIXING ROOM. HERE, TWO POUNDS (MAXIMUM) OF LEAD AZIDE WAS MIXED IN SEVEN SMALL POTS EACH SEPARATED FROM THE OTHERS BY THE STEEL WALLS PICTURED HERE. THE POTS ARE ALL FIXED TO A COMMON SHAFT WHICH IS ROTATED BY A HAND CRANK OUTSIDE OF ROOM (SEE HAER NJ-36A-26). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  10. Opportunities for increasing natural gas production in the near term. Volume VI. The East Cameron Block 271 Field. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1978-02-01

    This report examines the potential for increasing the rate of production of natural gas from the East Cameron Block 271 Field in the Gulf of Mexico Outer Continental Shelf. Proved reserves are estimated using all available reservoir data, including well logs and pressure tests, and cost parameters typical in the area. Alternative schedules for future production are devised, and net present values calculated from which the maximum production rate that also maximizes net present value is determined.

  11. Odd-Z Transactinide Compound Nucleus Reactions Including the Discovery of 260Bh

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Nelson, Sarah L.

    Several reactions producing odd-Z transactinide compound nuclei were studiedwith the 88-Inch Cyclotron and the Berkeley Gas-Filled Separator at the Lawrence Berkeley National Laboratory. The goal was to produce the same compound nucleus ator near the same excitation energy with similar values of angular momentum via differentnuclear reactions. In doing so, it can be determined if there is a preference in entrancechannel, because under these experimental conditions the survival portion of Swiatecki, Siwek-Wilcznska, and Wilczynski's"Fusion By Diffusion" model is nearly identical forthe two reactions. Additionally, because the same compound nucleus is produced, theexit channel is the same. Four compound nuclei were examined in this study: 258Db, 262Bh, 266Mt, and 272Rg. These nuclei were produced by using very similar heavy-ion induced-fusion reactions which differ only by one proton in the projectile or target nucleus (e.g.: 50Ti + 209Bi vs. 51V + 208Pb). Peak 1n exit channel cross sections were determined for each reaction in each pair, and three of the four pairs' cross sections were identical within statistical uncertainties. This indicates there is not an obvious preference of entrancechannel in these paired reactions. Charge equilibration immediately prior to fusionleading to a decreased fusion barrier is the likely cause of this phenomenon. In addition to this systematic study, the lightest isotope of element 107, bohrium, was discovered in the 209Bi( 52Cr,n) reaction. 260Bh was found to decay by emission of a 10.16 MeV alpha particle with a half-life of 35more » $$+19\\atop{-9}$$ ms. The cross section is 59 pb at an excitation energy of 15.0 MeV. The effect of the N = 152 shell is also seen in this isotope's alpha particle energy, the first evidence of such an effect in Bh. All reactions studied are also compared to model predictions by Swiatecki, Siwek-Wilcznska, and Wilczynski 's" Fusion By Diffusion " theory.« less

  12. Molecular characteristics and efficacy of 16D10 siRNAs in inhibiting root-knot nematode infection in transgenic grape hairy roots.

    PubMed

    Yang, Yingzhen; Jittayasothorn, Yingyos; Chronis, Demosthenis; Wang, Xiaohong; Cousins, Peter; Zhong, Gan-Yuan

    2013-01-01

    Root-knot nematodes (RKNs) infect many annual and perennial crops and are the most devastating soil-born pests in vineyards. To develop a biotech-based solution for controlling RKNs in grapes, we evaluated the efficacy of plant-derived RNA interference (RNAi) silencing of a conserved RKN effector gene, 16D10, for nematode resistance in transgenic grape hairy roots. Two hairpin-based silencing constructs, containing a stem sequence of 42 bp (pART27-42) or 271 bp (pART27-271) of the 16D10 gene, were transformed into grape hairy roots and compared for their small interfering RNA (siRNA) production and efficacy on suppression of nematode infection. Transgenic hairy root lines carrying either of the two RNAi constructs showed less susceptibility to nematode infection compared with control. Small RNA libraries from four pART27-42 and two pART27-271 hairy root lines were sequenced using an Illumina sequencing technology. The pART27-42 lines produced hundred times more 16D10-specific siRNAs than the pART27-271 lines. On average the 16D10 siRNA population had higher GC content than the 16D10 stem sequences in the RNAi constructs, supporting previous observation that plant dicer-like enzymes prefer GC-rich sequences as substrates for siRNA production. The stems of the 16D10 RNAi constructs were not equally processed into siRNAs. Several hot spots for siRNA production were found in similar positions of the hairpin stems in pART27-42 and pART27-271. Interestingly, stem sequences at the loop terminus produced more siRNAs than those at the stem base. Furthermore, the relative abundance of guide and passenger single-stranded RNAs from putative siRNA duplexes was largely correlated with their 5' end thermodynamic strength. This study demonstrated the feasibility of using a plant-derived RNAi approach for generation of novel nematode resistance in grapes and revealed several interesting molecular characteristics of transgene siRNAs important for optimizing plant RNAi constructs.

  13. Molecular Characteristics and Efficacy of 16D10 siRNAs in Inhibiting Root-Knot Nematode Infection in Transgenic Grape Hairy Roots

    PubMed Central

    Chronis, Demosthenis; Wang, Xiaohong; Cousins, Peter; Zhong, Gan-Yuan

    2013-01-01

    Root-knot nematodes (RKNs) infect many annual and perennial crops and are the most devastating soil-born pests in vineyards. To develop a biotech-based solution for controlling RKNs in grapes, we evaluated the efficacy of plant-derived RNA interference (RNAi) silencing of a conserved RKN effector gene, 16D10, for nematode resistance in transgenic grape hairy roots. Two hairpin-based silencing constructs, containing a stem sequence of 42 bp (pART27-42) or 271 bp (pART27-271) of the 16D10 gene, were transformed into grape hairy roots and compared for their small interfering RNA (siRNA) production and efficacy on suppression of nematode infection. Transgenic hairy root lines carrying either of the two RNAi constructs showed less susceptibility to nematode infection compared with control. Small RNA libraries from four pART27-42 and two pART27-271 hairy root lines were sequenced using an Illumina sequencing technology. The pART27-42 lines produced hundred times more 16D10-specific siRNAs than the pART27-271 lines. On average the 16D10 siRNA population had higher GC content than the 16D10 stem sequences in the RNAi constructs, supporting previous observation that plant dicer-like enzymes prefer GC-rich sequences as substrates for siRNA production. The stems of the 16D10 RNAi constructs were not equally processed into siRNAs. Several hot spots for siRNA production were found in similar positions of the hairpin stems in pART27-42 and pART27-271. Interestingly, stem sequences at the loop terminus produced more siRNAs than those at the stem base. Furthermore, the relative abundance of guide and passenger single-stranded RNAs from putative siRNA duplexes was largely correlated with their 5′ end thermodynamic strength. This study demonstrated the feasibility of using a plant-derived RNAi approach for generation of novel nematode resistance in grapes and revealed several interesting molecular characteristics of transgene siRNAs important for optimizing plant RNAi constructs. PMID:23874962

  14. Hyperekplexia mutation R271L of alpha1 glycine receptors potentiates allosteric interactions of nortropeines, propofol and glycine with [3H]strychnine binding.

    PubMed

    Maksay, Gábor; Bíró, Tímea; Laube, Bodo; Nemes, Péter

    2008-01-01

    Human alpha1 and hyperekplexia mutant alpha1(R271L) glycine receptors (GlyRs) were transiently expressed in human embryonic kidney 293 cells for [3H]strychnine binding. Binding parameters were determined using a ternary allosteric model. The hyperekplexia mutation increased the positive cooperativity of 0.3 mM propofol and glycine binding by about six times: the cooperativity factor beta was 0.26 for alpha1 GlyRs and 0.04 for alpha1(R271L) GlyRs. Thus, propofol restored the potency of glycine impaired by the mutation. Five nortropeines, i.e. substituted benzoates of nortropine and a new compound, nortropisetron were prepared and also examined on [3H]strychnine binding. They showed nanomolar displacing potencies amplified by the hyperekplexia mutation. The affinity of nor-O-zatosetron (2.6 nM) is one of the highest reported for GlyRs. This binding test offers an in vitro method to evaluate agents against neurological disorders associated with inherited mutations of GlyRs.

  15. Photodissociation dynamics of nitromethane at 226 and 271 nm at both nanosecond and femtosecond time scales.

    PubMed

    Guo, Y Q; Bhattacharya, A; Bernstein, E R

    2009-01-08

    Photodissociation of nitromethane has been investigated for decades both theoretically and experimentally; however, as a whole picture, the dissociation dynamics for nitromethane are still not clear, although many different mechanisms have been proposed. To make a complete interpretation of these different mechanisms, photolysis of nitromethane at 226 and 271 nm under both collisional and collisionless conditions is investigated at nanosecond and femtosecond time scales. These two laser wavelengths correspond to the pi* <-- pi and pi* <-- n excitations of nitromethane, respectively. In nanosecond 226 nm (pi* <-- pi) photolysis experiments, CH(3) and NO radicals are observed as major products employing resonance enhanced multiphoton ionization techniques and time-of-flight mass spectrometry. Additionally, OH and CH(3)O radicals are weakly observed as dissociation products employing laser induced fluorescence spectroscopy; the CH(3)O product is only observed under collisional conditions. In femtosecond 226 nm experiments, CH(3), NO(2), and NO products are observed. These results confirm that rupture of C-N bond should be the main primary process for the photolysis of nitromethane after the pi* <-- pi excitation at 226 nm, and the NO(2) molecule should be the precursor of the observed NO product. Formation of the CH(3)O radical after the recombination of CH(3) and NO(2) species under collisional conditions rules out a nitro-nitrite isomerization mechanism for the generation of CH(3)O and NO from pi pi* CH(3)NO(2). The OH radical formation for pi pi* CH(3)NO(2) should be a minor dissociation channel because of the weak OH signal in both nanosecond and femtosecond (nonobservable) experiments. Single color femtosecond pump-probe experiments at 226 nm are also employed to monitor the dynamics of the dissociation of nitromethane after the pi* <-- pi excitation. Because of the ultrafast dynamics of product formation at 226 nm, the pump-probe transients for the three dissociation products are measured as an autocorrelation of the laser pulse, indicating the dissociation of nitromethane in the pi pi* excited state is faster than the laser pulse duration (180 fs). In nanosecond 271 nm (pi* <-- n) photolysis experiments, pump-probe experiments are performed to detect potential dissociation products, such as CH(3), NO(2), CH(3)O, and OH; however, none of them is observed. In femtosecond 271 nm laser experiments, the nitromethane parent ion is observed with major intensity, together with CH(3), NO(2), and NO fragment ions with only minor intensities. Pump-probe transients for both nitromethane parent and fragment ions at 271 nm excitation and 406.5 nm ionization display a fast exponential decay with a constant time of 36 fs, which we suggest to be the lifetime of the excited n pi* state of nitromethane. Combined with the 271 nm nanosecond pump-probe experiments, in which none of the CH(3), NO(2), CH(3)O, or OH fragment is observed, we suggest that all the fragment ions generated in 271 nm femtosecond laser experiments are derived from the parent ion, and dissociation of nitromethane from the n pi* excited electronic state does not occur in a supersonic molecular beam under collisionless conditions.

  16. Identification of tetrapeptides from a mixture based positional scanning library that can restore nM full agonist function of the L106P, I69T, I102S, A219V, C271Y, and C271R human melanocortin-4 polymorphic receptors (hMC4Rs).

    PubMed

    Haslach, Erica M; Huang, Huisuo; Dirain, Marvin; Debevec, Ginamarie; Geer, Phaedra; Santos, Radleigh G; Giulianotti, Marc A; Pinilla, Clemencia; Appel, Jon R; Doering, Skye R; Walters, Michael A; Houghten, Richard A; Haskell-Luevano, Carrie

    2014-06-12

    Human obesity has been linked to genetic factors and single nucleotide polymorphisms (SNPs). Melanocortin-4 receptor (MC4R) SNPs have been associated with up to 6% frequency in morbidly obese children and adults. A potential therapy for individuals possessing such genetic modifications is the identification of molecules that can restore proper receptor signaling and function. These compounds could serve as personalized medications improving quality of life issues as well as alleviating diseases symptoms associated with obesity including type 2 diabetes. Several hMC4 SNP receptors have been pharmacologically characterized in vitro to have a decreased, or a lack of response, to endogenous agonists such as α-, β-, and γ2-melanocyte stimulating hormones (MSH) and adrenocorticotropin hormone (ACTH). Herein we report the use of a mixture based positional scanning combinatorial tetrapeptide library to discover molecules with nM full agonist potency and efficacy to the L106P, I69T, I102S, A219V, C271Y, and C271R hMC4Rs. The most potent compounds at all these hMC4R SNPs include Ac-His-(pI)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Arg-(pI)Phe-NH2, and Ac-Arg-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, revealing new ligand pharmacophore models for melanocortin receptor drug design strategies.

  17. Identification of Tetrapeptides from a Mixture Based Positional Scanning Library That Can Restore nM Full Agonist Function of the L106P, I69T, I102S, A219V, C271Y, and C271R Human Melanocortin-4 Polymorphic Receptors (hMC4Rs)

    PubMed Central

    2015-01-01

    Human obesity has been linked to genetic factors and single nucleotide polymorphisms (SNPs). Melanocortin-4 receptor (MC4R) SNPs have been associated with up to 6% frequency in morbidly obese children and adults. A potential therapy for individuals possessing such genetic modifications is the identification of molecules that can restore proper receptor signaling and function. These compounds could serve as personalized medications improving quality of life issues as well as alleviating diseases symptoms associated with obesity including type 2 diabetes. Several hMC4 SNP receptors have been pharmacologically characterized in vitro to have a decreased, or a lack of response, to endogenous agonists such as α-, β-, and γ2-melanocyte stimulating hormones (MSH) and adrenocorticotropin hormone (ACTH). Herein we report the use of a mixture based positional scanning combinatorial tetrapeptide library to discover molecules with nM full agonist potency and efficacy to the L106P, I69T, I102S, A219V, C271Y, and C271R hMC4Rs. The most potent compounds at all these hMC4R SNPs include Ac-His-(pI)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Arg-(pI)Phe-NH2, and Ac-Arg-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, revealing new ligand pharmacophore models for melanocortin receptor drug design strategies. PMID:24517312

  18. High-Throughput Analytical Techniques for Determination of Residues of 653 Multiclass Pesticides and Chemical Pollutants in Tea, Part VI: Study of the Degradation of 271 Pesticide Residues in Aged Oolong Tea by Gas Chromatography-Tandem Mass Spectrometry and Its Application in Predicting the Residue Concentrations of Target Pesticides.

    PubMed

    Chang, Qiao-Ying; Pang, Guo-Fang; Fan, Chun-Lin; Chen, Hui; Wang, Zhi-Bin

    2016-07-01

    The degradation rate of 271 pesticide residues in aged Oolong tea at two spray concentrations, named a and b (a < b), were monitored for 120 days using GC-tandem MS (GC-MS/MS). To research the degradation trends and establish regression equations, determination days were plotted as horizontal ordinates and the residue concentrations of pesticide were plotted as vertical ordinates. Here, we consider the degradation equations of 271 pesticides over 40 and 120 days, summarize the degradation rates in six aspects (A-F), and discuss the degradation trends of the 271 pesticides in aged Oolong tea in detail. The results indicate that >70% of the determined pesticides coincide with the degradation regularity of trends A, B, and E, i.e., the concentration of pesticide will decrease within 4 months. Next, 20 representative pesticides were selected for further study at higher spray concentrations, named c and d (d > c > b > a), in aged Oolong tea over another 90 days. The determination days were plotted on the x-axis, and the differences between each determined result and first-time-determined value of target pesticides were plotted on the y-axis. The logarithmic function was obtained by fitting the 90-day determination results, allowing the degradation value of a target pesticide on a specific day to be calculated. These logarithmic functions at d concentration were applied to predict the residue concentrations of pesticides at c concentration. Results revealed that 70% of the 20 pesticides had the lower deviation ratios of predicted and measured results.

  19. Multi-institutional analysis of CT and MRI reports evaluating indeterminate renal masses: comparison to a national survey investigating desired report elements.

    PubMed

    Hu, Eric M; Zhang, Andrew; Silverman, Stuart G; Pedrosa, Ivan; Wang, Zhen J; Smith, Andrew D; Chandarana, Hersh; Doshi, Ankur; Shinagare, Atul B; Remer, Erick M; Kaffenberger, Samuel D; Miller, David C; Davenport, Matthew S

    2018-04-17

    To determine the need for a standardized renal mass reporting template by analyzing reports of indeterminate renal masses and comparing their contents to stated preferences of radiologists and urologists. The host IRB waived regulatory oversight for this multi-institutional HIPAA-compliant quality improvement effort. CT and MRI reports created to characterize an indeterminate renal mass were analyzed from 6 community (median: 17 reports/site) and 6 academic (median: 23 reports/site) United States practices. Report contents were compared to a published national survey of stated preferences by academic radiologists and urologists from 9 institutions. Descriptive statistics and Chi-square tests were calculated. Of 319 reports, 85% (271; 192 CT, 79 MRI) reported a possibly malignant mass (236 solid, 35 cystic). Some essential elements were commonly described: size (99% [269/271]), mass type (solid vs. cystic; 99% [268/271]), enhancement (presence vs. absence; 92% [248/271]). Other essential elements had incomplete penetrance: the presence or absence of fat in solid masses (14% [34/236]), size comparisons when available (79% [111/140]), Bosniak classification for cystic masses (54% [19/35]). Preferred but non-essential elements generally were described in less than half of reports. Nephrometry scores usually were not included for local therapy candidates (12% [30/257]). Academic practices were significantly more likely than community practices to include mass characterization details, probability of malignancy, and staging. Community practices were significantly more likely to include management recommendations. Renal mass reporting elements considered essential or preferred often are omitted in radiology reports. Variation exists across radiologists and practice settings. A standardized template may mitigate these inconsistencies.

  20. Testosterone Deficiency and Bone Metabolism Damage in Testicular Cancer Survivors

    PubMed Central

    Ondrusova, Martina; Spanikova, Beata; Sevcikova, Katarina; Ondrus, Dalibor

    2016-01-01

    The aim of the study was to investigate the influence of therapeutic modalities and sexual hormone levels on changes in bone mineral density (BMD) in testicular cancer (TC) survivors. In a cross-sectional descriptive, long-term follow-up study, a total of 1,249 long-term TC survivors were evaluated according to treatment modality: orchiectomy (OE) only, OE + chemotherapy (CT), or OE + radiotherapy (RT). Luteinizing hormone (LH), total testosterone (TST), marker of bone resorption (β-carboxyl-terminal cross-linking telopeptide of type I collagen—CTx), and BMD were evaluated. Standard statistical techniques were used to test the differences between groups of patients. TST decrease was observed in 46/313 TC survivors after OE alone, in 103/665 after OE + CT, and in 66/271 after OE + RT. LH increase was observed in 23/313 TC survivors after OE alone, in 154/665 after OE + CT, and in 43/271 after OE + RT. CTx increase was observed in 116/313 TC survivors after OE alone, in 324/665 after OE + CT, and in 82/271 after OE + RT. Osteopenia/osteoporosis occurred in 136/313 TC survivors after OE alone, in 298/665 after OE + CT, and in 139/271 after OE + RT. TC survivors after RT have statistically significant decreased TST levels, increased LH and nonsignificant worse BMD (osteopenia/osteoporosis) in comparison with TC survivors after OE alone or CT. TST decrease and LH increase were statistically significant, more frequently observed in patients with osteopenia/osteoporosis. Examination of TST is an important part of follow-up in TC survivors with bilateral as well as unilateral disease. The important part of standard examination algorithm should be also the osteological examination of TC survivors mainly in patients with androgen deficiency. PMID:27489147

  1. In Vitro Activities of Gemifloxacin versus Five Quinolones and Two Macrolides against 271 Spanish Isolates of Legionella pneumophila: Influence of Charcoal on Susceptibility Test Results

    PubMed Central

    García, M. T.; Pelaz, C.; Giménez, M. J.; Aguilar, L.

    2000-01-01

    The MICs at which 90% of isolates are inhibited for gemifloxacin, trovafloxacin, and grepafloxacin were low (≤0.01 μg/ml) for 271 Legionella isolates when they were determined by the broth microdilution method but increased (≥6 dilutions) when they were determined by the agar dilution method. This was due to the charcoal in the agar dilution medium, as shown by the progressive decrease in the MICs when the charcoal concentrations decreased. As free drug is the active fraction, charcoal binding should be considered. PMID:10898695

  2. Rates of upgrade to malignancy for 271 cases of flat epithelial atypia (FEA) diagnosed by breast core biopsy.

    PubMed

    Peres, Alexandre; Barranger, Emmanuel; Becette, Véronique; Boudinet, Alain; Guinebretiere, Jean-Marc; Cherel, Pascal

    2012-06-01

    Flat epithelial atypia (FEA) is a borderline lesion that might represent an early stage in the development of certain low-grade carcinomas in situ and invasive cancers. There are no guidelines on its management. Our objectives were to determine the upgrade to malignancy rate and identify a subpopulation of patients that might undergo just mammographic surveillance. We retrospectively reviewed the data for 271 FEA cases among 5,555 breast core biopsies obtained over a 7-year period (January 2003-2010). We collated clinical data (age, history of cancer, menopausal status), radiological data (lesion type, size, Bi-Rads category), technical data (number of biopsies, needle gauge, excision quality) and histological data and sought correlations between these factors and upgrade rate. The 271 FEA comprised 128 cases of pure FEA, 135 cases of FEA + atypical ductal hyperplasia, and 8 cases of FEA + atypical lobular hyperplasia. Overall, 184 patients underwent surgery and 46 mammographic surveillance. Surgery detected 34 cases of malignancy (23 CIS, 7 invasive cancers, and 4 mixed cases) giving a 15% upgrade rate. Quality of excision was the only factor associated with under-diagnosis. The presence of FEA at biopsy warrants surgery.

  3. Structural and functional characterization of a ubiquitin variant engineered for tight and specific binding to an alpha-helical ubiquitin interacting motif.

    PubMed

    Manczyk, Noah; Yates, Bradley P; Veggiani, Gianluca; Ernst, Andreas; Sicheri, Frank; Sidhu, Sachdev S

    2017-05-01

    Ubiquitin interacting motifs (UIMs) are short α-helices found in a number of eukaryotic proteins. UIMs interact weakly but specifically with ubiquitin conjugated to other proteins, and in so doing, mediate specific cellular signals. Here we used phage display to generate ubiquitin variants (UbVs) targeting the N-terminal UIM of the yeast Vps27 protein. Selections yielded UbV.v27.1, which recognized the cognate UIM with high specificity relative to other yeast UIMs and bound with an affinity more than two orders of magnitude higher than that of ubiquitin. Structural and mutational studies of the UbV.v27.1-UIM complex revealed the molecular details for the enhanced affinity and specificity of UbV.v27.1, and underscored the importance of changes at the binding interface as well as at positions that do not contact the UIM. Our study highlights the power of the phage display approach for selecting UbVs with unprecedented affinity and high selectivity for particular α-helical UIM domains within proteomes, and it establishes a general approach for the development of inhibitors targeting interactions of this type. © 2017 The Protein Society.

  4. Structural analysis of an HLA-B27 functional variant, B27d detected in American blacks

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rojo, S.; Aparicio, P.; Hansen, J.A.

    1987-11-15

    The structure of a new functional variant B27d has been established by comparative peptide mapping and radiochemical sequencing. This analysis complete the structural characterization of the six know histocompatibility leukocyte antigen (HLA)-B27 subtypes. The only detected amino acid change between the main HLA-B27.1 subtype and B27d is that of Try/sub 59/ to His/sub 59/. Position 59 has not been previously found to vary among class I HLA or H-2 antigens. Such substitution accounts for the reported isoelectric focusing pattern of this variant. HLA-B27d is the only B27 variant found to differ from other subtypes by a single amino acid replacement.more » The nature of the change is compatible with its origin by a point mutation from HLB-B27.1. Because B27d was found only American blacks and in no other ethnic groups, it is suggested that this variant originated as a result of a mutation of the B27.1 gene that occurred within the black population. Structural analysis of B27d was done by comparative mapping. Radiochemical sequencing was carried out with /sup 14/C-labeled and /sup 3/H-labeled amino acids.« less

  5. CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization

    PubMed Central

    Tormin, Ariane; Li, Ou; Brune, Jan Claas; Walsh, Stuart; Schütz, Birgit; Ehinger, Mats; Ditzel, Nicholas; Kassem, Moustapha

    2011-01-01

    Nonhematopoietic bone marrow mesenchymal stem cells (BM-MSCs) are of central importance for bone marrow stroma and the hematopoietic environment. However, the exact phenotype and anatomical distribution of specified MSC populations in the marrow are unknown. We characterized the phenotype of primary human BM-MSCs and found that all assayable colony-forming units-fibroblast (CFU-Fs) were highly and exclusively enriched not only in the lin−/CD271+/CD45−/CD146+ stem-cell fraction, but also in lin−/CD271+/CD45−/CD146−/low cells. Both populations, regardless of CD146 expression, shared a similar phenotype and genotype, gave rise to typical cultured stromal cells, and formed bone and hematopoietic stroma in vivo. Interestingly, CD146 was up-regulated in normoxia and down-regulated in hypoxia. This was correlated with in situ localization differences, with CD146 coexpressing reticular cells located in perivascular regions, whereas bone-lining MSCs expressed CD271 alone. In both regions, CD34+ hematopoietic stem/progenitor cells were located in close proximity to MSCs. These novel findings show that the expression of CD146 differentiates between perivascular versus endosteal localization of non-hematopoietic BM-MSC populations, which may be useful for the study of the hematopoietic environment. PMID:21415267

  6. Improved penicillin amidase production using a genetically engineered mutant of escherichia coli ATCC 11105

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Robas, N.; Zouheiry, H.; Branlant, G.

    Penicillin G amidase (PGA) is a key enzyme for the industrial production of penicillin G derivatives used in therapeutics. Escherichia coli ATCC 11105 is the more commonly used strain for PGA production. To improve enzyme yield, the authors constructed various recombinant E. coli HB 101 and ATCC 11105 strains. For each strain, PGA production was determined for various concentrations of glucose and phenylacetic acid (PAA) in the medium. The E. coli strain, G271, was identified as the best performer (800 U NIPAB/L). This strain was obtained as follows: an E. coli ATCC 11105 mutant (E. coli G133) was first selectedmore » based on a low negative effect of glucose on PGA production. This mutant was then transformed with a pBR322 derivative containing the PGA gene. Various experiments were made to try to understand the reason for the high productivity of E. coli G271. The host strain, E. coli G133, was found to be mutated in one (or more) gene(s) whose product(s) act(s) in trans on the PGA gene expression. Its growth is not inhibited by high glucose concentration in the medium. Interestingly, whereas glucose still exerts some negative effect on the PGA production by E. coli G133, PGA production by its transformant (E. coli G271) is stimulated by glucose. The reason for this stimulation is discussed. Transformation of E. coli G133 with a pBR322 derivative containing the HindIII fragment of the PGA gene, showed that the performance of E. coli G271 depends both upon the host strain properties and the plasmid structure. Study of the production by the less efficient E. coli HB101 derivatives brought some light on the mechanism of regulation of the PGA gene.« less

  7. Three-dimensional structure of phosphoribosyl pyrophosphate synthetase from E. coli at 2.71 Å resolution

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Timofeev, V. I., E-mail: inna@ns.crys.ras.ru, E-mail: tostars@mail.ru, E-mail: ugama@yandex.ru; Abramchik, Yu. A.; Zhukhlistova, N. E.

    2016-01-15

    Phosphoribosyl pyrophosphate synthetase from Escherichia coli was cloned, purified, and crystallized. Single crystals of the enzyme were grown under microgravity. The X-ray diffraction data set was collected at the Spring-8 synchrotron facility and used to determine the three-dimensional structure of the enzyme by the molecular-replacement method at 2.71 Å resolution. The active and regulatory sites in the molecule of E. coli phosphoribosyl pyrophosphate synthetase were revealed by comparison with the homologous protein from Bacillus subtilis, the structure of which was determined in a complex with functional ligands. The conformations of polypeptide-chain fragments surrounding and composing the active and regulatory sitesmore » were shown to be identical in both proteins.« less

  8. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... alone, or in conjunction with another access device, to obtain payments, allotments, benefits, money... retail food business operated from a truck, bus, pushcart, or other mobile vehicle. Identification (ID...

  9. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... alone, or in conjunction with another access device, to obtain payments, allotments, benefits, money... retail food business operated from a truck, bus, pushcart, or other mobile vehicle. Identification (ID...

  10. 7 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... alone, or in conjunction with another access device, to obtain payments, allotments, benefits, money... retail food business operated from a truck, bus, pushcart, or other mobile vehicle. Identification (ID...

  11. Incidence of complications associated with tibial tuberosity advancement in Boxer dogs.

    PubMed

    de Lima Dantas, Brigite; Sul, Rui; Parkin, Tim; Calvo, Ignacio

    2016-01-01

    To retrospectively review and describe the incidence of complications associated with tibial tuberosity advancement (TTA) surgical procedures in a group of Boxer dogs (n = 36 stifles) and compare the data with a non-Boxer control population (n = 271 stifles). Retrospective analysis of medical records to identify all dogs that underwent TTA surgery due to cranial cruciate ligament disease. These records were categorized into two groups: Boxer dogs and non-Boxer dogs (controls - all other breeds). Of the 307 stifles included, 69 complications were reported in 58 joints. The complication rate differed significantly for Boxer dogs (16/36 stifles) and non-Boxer dogs (42/271 stifles), corresponding to an odds ratio of 5.8 (confidence interval: 1.96-17.02; p-value <0.001). Boxer dogs were more likely to undergo revision surgery and to develop multiple complications. The incidence of tibial tuberosity fractures requiring surgical repair (2/36 versus 1/271) and incisional infections requiring antibiotic treatment (three in each group) was significantly higher in the Boxer group. Boxer dogs had more major and multiple complications after TTA surgery than the control non-Boxer group; these complications included higher rates of revision surgery, tibial tuberosity fractures requiring stabilization, and infection related complications. The pertinence and value of breed-specific recommendations for cranial cruciate ligament disease appears to be a subject worthy of further investigation.

  12. Self-preservation and structural transition of gas hydrates during dissociation below the ice point: an in situ study using Raman spectroscopy

    NASA Astrophysics Data System (ADS)

    Zhong, Jin-Rong; Zeng, Xin-Yang; Zhou, Feng-He; Ran, Qi-Dong; Sun, Chang-Yu; Zhong, Rui-Qin; Yang, Lan-Ying; Chen, Guang-Jin; Koh, Carolyn A.

    2016-12-01

    The hydrate structure type and dissociation behavior for pure methane and methane-ethane hydrates at temperatures below the ice point and atmospheric pressure were investigated using in situ Raman spectroscopic analysis. The self-preservation effect of sI methane hydrate is significant at lower temperatures (268.15 to 270.15 K), as determined by the stable C-H region Raman peaks and AL/AS value (Ratio of total peak area corresponding to occupancies of guest molecules in large cavities to small cavities) being around 3.0. However, it was reduced at higher temperatures (271.15 K and 272.15 K), as shown from the dramatic change in Raman spectra and fluctuations in AL/AS values. The self-preservation effect for methane-ethane double hydrate is observed at temperatures lower than 271.15 K. The structure transition from sI to sII occurred during the methane-ethane hydrate decomposition process, which was clearly identified by the shift in peak positions and the change in relative peak intensities at temperatures from 269.15 K to 271.15 K. Further investigation shows that the selectivity for self-preservation of methane over ethane leads to the structure transition; this kind of selectivity increases with decreasing temperature. This work provides new insight into the kinetic behavior of hydrate dissociation below the ice point.

  13. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... this part are designed and promulgated to establish minimum standards which local zoning bylaws must... commercial and industrial use, other than existing commercial or industrial use not inconsistent with the...

  14. Brief Report: Autistic Features in Children and Adolescents with Gender Dysphoria.

    PubMed

    Skagerberg, Elin; Di Ceglie, Domenico; Carmichael, Polly

    2015-08-01

    This paper looks at the association between gender dysphoria (GD), scores on the Social Responsiveness Scale (SRS), and reported diagnoses of autism spectrum disorder (ASD). Parents of 166 young people presenting with GD (Mean age = 14.26, SD = 2.68) completed the SRS. Information concerning an ASD diagnosis was also extracted from the patient files. 45.8% fell within the normal range on the SRS and of those 2.8% had an ASD diagnosis. 27.1% fell within the mild/moderate range and of those 15.6% had an ASD diagnosis and 6.7% an ASD query. 27.1% fell within the severe range and of those 24.4% had an ASD diagnosis and 26.7% an ASD query. No difference was found in autistic features between the natal females and males.

  15. After Opportunity's First Drive in Six Weeks

    NASA Technical Reports Server (NTRS)

    2007-01-01

    NASA's Mars Exploration Rover Opportunity used its front hazard-identification camera to obtain this image at the end of a drive on the rover's 1,271st sol, or Martian day (Aug. 21, 2007).

    Due to sun-obscuring dust storms limiting the rover's supply of solar energy, Opportunity had not driven since sol 1,232 (July 12, 2007). On sol 1,271, after the sky above Opportunity had been gradually clearing for more than two weeks, the rover rolled 13.38 meters (44 feet). Wheel tracks are visible in front of the rover because the drive ended with a short test of driving backwards.

    Opportunity's turret of four tools at the end of the robotic arm fills the center of the image. Victoria Crater, site of the rover's next science targets, lies ahead.

  16. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... concerned individuals or groups. This shall not include complaints alleging discrimination on the basis of..., Pennsylvania, Puerto Rico, Virginia, the Virgin Islands of the United States, and West Virginia: Mid-Atlantic...

  17. 40 CFR 421.271 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Rare Earth Metals... rare earths to about 94-99 percent. The balance of tha alloy includes traces of other elements and one...

  18. 10 CFR 600.250 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Programs (SF-271) (as applicable). (3) Final request for payment (SF-270) (if applicable). (4) Invention... extend this timeframe. These may include but are not limited to: (1) Final performance or progress report...

  19. 13 CFR 143.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Programs (SF-271) (as applicable). (3) Final request for payment (SF-270) (if applicable). (4) Invention... extend this timeframe. These may include but are not limited to: (1) Final performance or progress report...

  20. 15 CFR 24.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Programs (SF-271) (as applicable). (3) Final request for payment (SF-270) (if applicable). (4) Invention... extend this timeframe. These may include but are not limited to: (1) Final performance or progress report...

  1. 14 CFR 1273.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Programs (SF-271) (as applicable). (3) Final request for payment (SF-270) (if applicable). (4) Invention... extend this timeframe. These may include but are not limited to: (1) Final performance or progress report...

  2. 41 CFR 101-27.102-1 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 27-INVENTORY MANAGEMENT 27.1... having recurring demands, except items held at points of final use, in accordance with the economic order...

  3. First State National Historical Park Act

    THOMAS, 112th Congress

    Sen. Carper, Thomas R. [D-DE

    2011-02-10

    Senate - 01/13/2012 Placed on Senate Legislative Calendar under General Orders. Calendar No. 271. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  4. 40 CFR 124.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 271.2 (RCRA), the definitions below apply to this part, except for PSD permits which are governed by... Administrator delegates authority to the Environmental Appeals Board to issue final decisions in RCRA, PSD, UIC...

  5. 40 CFR 124.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 271.2 (RCRA), the definitions below apply to this part, except for PSD permits which are governed by... Administrator delegates authority to the Environmental Appeals Board to issue final decisions in RCRA, PSD, UIC...

  6. 40 CFR 124.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 271.2 (RCRA), the definitions below apply to this part, except for PSD permits which are governed by... Administrator delegates authority to the Environmental Appeals Board to issue final decisions in RCRA, PSD, UIC...

  7. 40 CFR 124.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 271.2 (RCRA), the definitions below apply to this part, except for PSD permits which are governed by... Administrator delegates authority to the Environmental Appeals Board to issue final decisions in RCRA, PSD, UIC...

  8. 40 CFR 124.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 271.2 (RCRA), the definitions below apply to this part, except for PSD permits which are governed by... Administrator delegates authority to the Environmental Appeals Board to issue final decisions in RCRA, PSD, UIC...

  9. 40 CFR 408.271 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Steamed and Canned Oyster... this chapter shall apply to this subpart. (b) The term product shall mean the weight of the oyster meat...

  10. 14 CFR 21.271 - Airworthiness approval tags.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... may issue an airworthiness approval tag (FAA Form 8130-3) for each engine and propeller covered by... shall be stamped on the engine or propeller identification data place instead of issuing an...

  11. 12 CFR 271.7 - Exemptions from disclosure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... its statutory functions; (2) Interfere with the orderly conduct of the foreign affairs of the United States; (3) Permit speculators or others to gain unfair profits or other unfair advantages by speculative...

  12. 77 FR 271 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    ... Threat Reduction Agency, 1680 Texas Street SE., Kirtland Air Force Base, Albuquerque, NM 87117-5669... Threat Reduction Agency, 1680 Texas Street SE., Kirtland Air Force Base, Albuquerque, NM 87117-5669...

  13. 48 CFR 1352.271-88 - Guarantees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... when practicable, the Government shall afford the contractor an opportunity to effect such corrections... expiration of the time frame stated in this clause, it is not subject to the time limitations stated in this...

  14. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Occupational Safety and Health Administration, U.S. Department of Labor, 200 Constitution Avenue, NW, Room... Administration, U.S. Department of Labor, John F. Kennedy Federal Building, Room E-340, Boston, Massachusetts...

  15. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., Occupational Safety and Health Administration, U.S. Department of Labor, 200 Constitution Avenue, NW, Room... Administration, U.S. Department of Labor, John F. Kennedy Federal Building, Room E-340, Boston, Massachusetts...

  16. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... agricultural economy, as well as result in more orderly marketing and distribution of foods. To alleviate such... households to obtain a more nutritious diet through normal channels of trade by increasing food purchasing...

  17. 14 CFR 21.271 - Airworthiness approval tags.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... (FAA Form 8130-3) for each engine and propeller covered by § 21.251(b)(4), and may issue an... Limitation Record, the production certification number shall be stamped on the engine or propeller...

  18. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... may have information about the matter. Interviews may be conducted by written correspondence, telephone or personal interview. (c) If circumstances warrant, the district director may also conduct an...

  19. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  20. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  1. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  2. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  3. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... For other contracts, such as those for hardware or software development, the procurement officer may... during the life of the contract at defined intervals on a provisional basis. If the final award fee...

  4. Hemolytic uremic syndrome (HUS) secondary to cobalamin C (cblC) disorder.

    PubMed

    Sharma, Ajay P; Greenberg, Cheryl R; Prasad, Asuri N; Prasad, Chitra

    2007-12-01

    Diarrhea-positive hemolytic uremic syndrome (HUS) is a common cause of acute renal failure in children. Diarrhea-negative (D-), or atypical HUS, is etiologically distinct. A Medline search identified seven previously reported D- cases of HUS secondary to cobalamin C (cblC) disease presenting in infancy. An infantile presentation is reported to be associated with a high mortality rate (6/7 cases). We describe the results of a 5-year longitudinal follow-up in a child diagnosed with D- HUS secondary to cblC disease in infancy. Mutation analysis in this patient identified homozygosity for the 271 dupA mutation (c.271 dupA) in the cblC MMACHC gene. We briefly review the published experience in cblC-associated HUS to highlight the clinical characteristics of this uncommon, but potentially treatable, condition.

  5. Multiple viral/self immunological cross-reactivity in liver kidney microsomal antibody positive hepatitis C virus infected patients is associated with the possession of HLA B51.

    PubMed

    Bogdanos, D-P; Lenzi, M; Okamoto, M; Rigopoulou, E I; Muratori, P; Ma, Y; Muratori, L; Tsantoulas, D; Mieli- Vergani, G; Bianchi, F B; Vergani, D

    2004-01-01

    Liver Kidney Microsomal autoantibody type 1(LKM1) directed to cytochrome P4502D6 (CYP2D6) characterises autoimmune hepatitis type-2 (AIH-2), but is also found in a proportion of chronic hepatitis C virus (HCV) infected patients, CYP2D6252-271 being a major B- cell autoepitope. Molecular mimicry and immunological cross-reactivity between CYP2D6252-271, HCV polyprotein and the infected cell protein 4 (ICP4) of herpes simplex virus type 1 (HSV-1) have been suggested as triggers for the induction of LKM1, but reactivity and cross-reactivity to the relevant sequences have not been investigated experimentally. CYP2D6252-271 and its viral homologues were constructed and tested by ELISA in the sera of 46 chronically infected HCV patients, 23 of whom were LKM1 positive. Reactivity to the E1 HCV and ICP4 HSV1 mimics was frequently found in HCV infected patients irrespectively of their LKM1 status; viral/self cross-reactivity (as indicated by inhibition studies), however, was present in the only 2 of the 23 LKM1 seropositive HCV patients, who possessed the HLA allotype B51. Our results indicate that in HCV infected patients virus/self cross-reactivity is dependent on a specific immunogenetic background, a finding awaiting confirmation by studies in larger series of patients.

  6. Self-preservation and structural transition of gas hydrates during dissociation below the ice point: an in situ study using Raman spectroscopy

    PubMed Central

    Zhong, Jin-Rong; Zeng, Xin-Yang; Zhou, Feng-He; Ran, Qi-Dong; Sun, Chang-Yu; Zhong, Rui-Qin; Yang, Lan-Ying; Chen, Guang-Jin; Koh, Carolyn A.

    2016-01-01

    The hydrate structure type and dissociation behavior for pure methane and methane-ethane hydrates at temperatures below the ice point and atmospheric pressure were investigated using in situ Raman spectroscopic analysis. The self-preservation effect of sI methane hydrate is significant at lower temperatures (268.15 to 270.15 K), as determined by the stable C-H region Raman peaks and AL/AS value (Ratio of total peak area corresponding to occupancies of guest molecules in large cavities to small cavities) being around 3.0. However, it was reduced at higher temperatures (271.15 K and 272.15 K), as shown from the dramatic change in Raman spectra and fluctuations in AL/AS values. The self-preservation effect for methane-ethane double hydrate is observed at temperatures lower than 271.15 K. The structure transition from sI to sII occurred during the methane-ethane hydrate decomposition process, which was clearly identified by the shift in peak positions and the change in relative peak intensities at temperatures from 269.15 K to 271.15 K. Further investigation shows that the selectivity for self-preservation of methane over ethane leads to the structure transition; this kind of selectivity increases with decreasing temperature. This work provides new insight into the kinetic behavior of hydrate dissociation below the ice point. PMID:27941857

  7. Self-preservation and structural transition of gas hydrates during dissociation below the ice point: an in situ study using Raman spectroscopy.

    PubMed

    Zhong, Jin-Rong; Zeng, Xin-Yang; Zhou, Feng-He; Ran, Qi-Dong; Sun, Chang-Yu; Zhong, Rui-Qin; Yang, Lan-Ying; Chen, Guang-Jin; Koh, Carolyn A

    2016-12-12

    The hydrate structure type and dissociation behavior for pure methane and methane-ethane hydrates at temperatures below the ice point and atmospheric pressure were investigated using in situ Raman spectroscopic analysis. The self-preservation effect of sI methane hydrate is significant at lower temperatures (268.15 to 270.15 K), as determined by the stable C-H region Raman peaks and A L /A S value (Ratio of total peak area corresponding to occupancies of guest molecules in large cavities to small cavities) being around 3.0. However, it was reduced at higher temperatures (271.15 K and 272.15 K), as shown from the dramatic change in Raman spectra and fluctuations in A L /A S values. The self-preservation effect for methane-ethane double hydrate is observed at temperatures lower than 271.15 K. The structure transition from sI to sII occurred during the methane-ethane hydrate decomposition process, which was clearly identified by the shift in peak positions and the change in relative peak intensities at temperatures from 269.15 K to 271.15 K. Further investigation shows that the selectivity for self-preservation of methane over ethane leads to the structure transition; this kind of selectivity increases with decreasing temperature. This work provides new insight into the kinetic behavior of hydrate dissociation below the ice point.

  8. Arctic Dinoflagellate Migration Marks the Oligocene Glacial Maximum: Implications for the Rupelian-Chattian Boundary

    NASA Astrophysics Data System (ADS)

    van Simaeys, S.; Brinkhuis, H.; Pross, J.; Williams, G. L.; Zachos, J. C.

    2004-12-01

    Various geochemical and biotic climate proxies, and notably deep-sea benthic foraminiferal δ 18O records indicate that the Eocene 'greenhouse' state of the Earth gradually evolved towards an earliest Oligocene 'icehouse' state, eventually triggering the abrupt appearance of large continental ice-sheets on Antarctic at ˜33.3 Ma (Oi-1 event). This, however, was only the first of two major glacial events in the Oligocene. Benthic foraminiferal δ 18O records show a second positive excursion in the mid Oligocene, consistent with a significant ice-sheet expansion and/or cooling at 27.1 Ma (Oi-2b) coincident with magnetosubchron C9n. Here, we report on a mid Oligocene, globally synchronous, Arctic dinoflagellate migration event, calibrated against the upper half of C9n. A sudden appearance, and abundance increases of the Arctic taxon Svalbardella at lower-middle latitudes coincides with the so-called Oi-2b benthic δ 18O event, dated at ˜27.1 Ma. This phenomenon is taken to indicate significant high-latitude surface water cooling, concomitant Antarctic ice-sheet growth, and sea level lowering. The duration of the Svalbardella migrations, and the episode of profound cooling is estimated as ˜500 ka, and is here termed the Oligocene Glacial Maximum (OGM). Our records suggest a close link between the OGM, sea-level fall, and the classic Rupelian-Chattian boundary, magnetostratigraphically dating this boundary as ˜27.1 Ma.

  9. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Methyl Bromide 54 FR 41402-408 Apr. 6, 1990. Dec. 11, 1989 Listing Certain Hydrocarbons Produced by Free Radical Catalyzed Processes 54 FR 50968-978 June 11, 1990. Mar. 29, 1990 Toxicity characteristic 55 FR...

  10. 40 CFR 271.1 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Methyl Bromide 54 FR 41402-408 Apr. 6, 1990. Dec. 11, 1989 Listing Certain Hydrocarbons Produced by Free Radical Catalyzed Processes 54 FR 50968-978 June 11, 1990. Mar. 29, 1990 Toxicity characteristic 55 FR...

  11. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  12. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  13. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  14. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  15. 40 CFR 271.21 - Procedures for revision of State programs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... immediate final decision takes effect or reverses the decision. (4) The procedures for proposed and final... Monitoring Activities, Methods Innovation Rule July 14, 2005 70 FR 34538, June 14, 2005 Process Vent and...

  16. 12 CFR 271.6 - Processing requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ten working days of receipt of the request. In exceptional situations, the Secretary of the Committee... Committee, 20th and C Street, N.W., Washington, D.C. 20551; or sent by facsimile to the Secretary of the...

  17. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... individuals of any cetacean species (not including mother/calf pairs, unless of species of concern listed in... one another. (ii) A single individual or mother/calf pair of any of the following marine mammals of...

  18. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Department of the Navy. The welder's qualifications shall be appropriate for the particular service... waste material or rubbish caused by contractor employees or the work, and at the completion of the work...

  19. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Department of the Navy. The welder's qualifications shall be appropriate for the particular service... waste material or rubbish caused by contractor employees or the work, and at the completion of the work...

  20. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Department of the Navy. The welder's qualifications shall be appropriate for the particular service... waste material or rubbish caused by contractor employees or the work, and at the completion of the work...

  1. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Department of the Navy. The welder's qualifications shall be appropriate for the particular service... waste material or rubbish caused by contractor employees or the work, and at the completion of the work...

  2. 48 CFR 1352.271-70 - Inspection and manner of doing work.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Department of the Navy. The welder's qualifications shall be appropriate for the particular service... waste material or rubbish caused by contractor employees or the work, and at the completion of the work...

  3. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... available data pertaining to the number of people participating in the program and the amounts of benefits... for completing the processing of such cases shall be five calendar days or the end of the month of...

  4. 75 FR 20985 - North Pacific Fishery Management Council; Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-22

    .... SUMMARY: The North Pacific Fishery Management Council's Crab Plan Team (CPT) will meet in Alaska on May 10... Council; telephone: (907) 271-2809. SUPPLEMENTARY INFORMATION: The Plan Team will discuss recent Council...

  5. 78 FR 70016 - North Pacific Fishery Management Council; Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-22

    ...) Implementation Team will meet in Anchorage, AK. DATES: The meeting will be held on December 9, 2013, from 8:30 a..., telephone: (907) 271-2809. SUPPLEMENTARY INFORMATION: The Team will review: [[Page 70017

  6. Mobile Technologies and Geographic Information Systems to Improve Health Care Systems: A Literature Review

    PubMed Central

    2014-01-01

    Background A growing body of research has employed mobile technologies and geographic information systems (GIS) for enhancing health care and health information systems, but there is yet a lack of studies of how these two types of systems are integrated together into the information infrastructure of an organization so as to provide a basis for data analysis and decision support. Integration of data and technical systems across the organization is necessary for efficient large-scale implementation. Objective The aim of this paper is to identify how mobile technologies and GIS applications have been used, independently as well as in combination, for improving health care. Methods The electronic databases PubMed, BioMed Central, Wiley Online Library, Scopus, Science Direct, and Web of Science were searched to retrieve English language articles published in international academic journals after 2005. Only articles addressing the use of mobile or GIS technologies and that met a prespecified keyword strategy were selected for review. Results A total of 271 articles were selected, among which 220 concerned mobile technologies and 51 GIS. Most articles concern developed countries (198/271, 73.1%), and in particular the United States (81/271, 29.9%), United Kingdom (31/271, 11.4%), and Canada (14/271, 5.2%). Applications of mobile technologies can be categorized by six themes: treatment and disease management, data collection and disease surveillance, health support systems, health promotion and disease prevention, communication between patients and health care providers or among providers, and medical education. GIS applications can be categorized by four themes: disease surveillance, health support systems, health promotion and disease prevention, and communication to or between health care providers. Mobile applications typically focus on using text messaging (short message service, SMS) for communication between patients and health care providers, most prominently reminders and advice to patients. These applications generally have modest benefits and may be appropriate for implementation. Integration of health data using GIS technology also exhibit modest benefits such as improved understanding of the interplay of psychological, social, environmental, area-level, and sociodemographic influences on physical activity. The studies evaluated showed promising results in helping patients treating different illnesses and managing their condition effectively. However, most studies use small sample sizes and short intervention periods, which means limited clinical or statistical significance. Conclusions A vast majority of the papers report positive results, including retention rate, benefits for patients, and economic gains for the health care provider. However, implementation issues are little discussed, which means the reasons for the scarcity of large-scale implementations, which might be expected given the overwhelmingly positive results, are yet unclear. There is also little combination between GIS and mobile technologies. In order for health care processes to be effective they must integrate different kinds of existing technologies and data. Further research and development is necessary to provide integration and better understand implementation issues. PMID:25099368

  7. Mobile technologies and geographic information systems to improve health care systems: a literature review.

    PubMed

    Nhavoto, José António; Grönlund, Ake

    2014-05-08

    A growing body of research has employed mobile technologies and geographic information systems (GIS) for enhancing health care and health information systems, but there is yet a lack of studies of how these two types of systems are integrated together into the information infrastructure of an organization so as to provide a basis for data analysis and decision support. Integration of data and technical systems across the organization is necessary for efficient large-scale implementation. The aim of this paper is to identify how mobile technologies and GIS applications have been used, independently as well as in combination, for improving health care. The electronic databases PubMed, BioMed Central, Wiley Online Library, Scopus, Science Direct, and Web of Science were searched to retrieve English language articles published in international academic journals after 2005. Only articles addressing the use of mobile or GIS technologies and that met a prespecified keyword strategy were selected for review. A total of 271 articles were selected, among which 220 concerned mobile technologies and 51 GIS. Most articles concern developed countries (198/271, 73.1%), and in particular the United States (81/271, 29.9%), United Kingdom (31/271, 11.4%), and Canada (14/271, 5.2%). Applications of mobile technologies can be categorized by six themes: treatment and disease management, data collection and disease surveillance, health support systems, health promotion and disease prevention, communication between patients and health care providers or among providers, and medical education. GIS applications can be categorized by four themes: disease surveillance, health support systems, health promotion and disease prevention, and communication to or between health care providers. Mobile applications typically focus on using text messaging (short message service, SMS) for communication between patients and health care providers, most prominently reminders and advice to patients. These applications generally have modest benefits and may be appropriate for implementation. Integration of health data using GIS technology also exhibit modest benefits such as improved understanding of the interplay of psychological, social, environmental, area-level, and sociodemographic influences on physical activity. The studies evaluated showed promising results in helping patients treating different illnesses and managing their condition effectively. However, most studies use small sample sizes and short intervention periods, which means limited clinical or statistical significance. A vast majority of the papers report positive results, including retention rate, benefits for patients, and economic gains for the health care provider. However, implementation issues are little discussed, which means the reasons for the scarcity of large-scale implementations, which might be expected given the overwhelmingly positive results, are yet unclear. There is also little combination between GIS and mobile technologies. In order for health care processes to be effective they must integrate different kinds of existing technologies and data. Further research and development is necessary to provide integration and better understand implementation issues.

  8. Radiobiological characteristics of cancer stem cells from esophageal cancer cell lines

    PubMed Central

    Wang, Jian-Lin; Yu, Jing-Ping; Sun, Zhi-Qiang; Sun, Su-Ping

    2014-01-01

    AIM: To study the cancer stem cell population in esophageal cancer cell lines KYSE-150 and TE-1 and identify whether the resulting stem-like spheroid cells display cancer stem cells and radiation resistance characteristics. METHODS: A serum-free medium (SFM) suspension was used to culture esophageal cancer stem cell lines and enrich the esophageal stem-like spheres. A reverse transcription polymerase chain reaction assay was used to detect stem cell gene expression in the spheroid cells. Radiosensitivity of stem-like spheres and parental cells were evaluated by clonogenic assays. Furthermore, different cells after different doses of irradiation were tested to evaluate the change in sphere formation, cell cycle and CD44+CD271+ expression of tumor stem-like spheroid cells using flow cytometry before and after irradiation. RESULTS: The cells were observed to generate an increased number of spheres in SFM with increasing cell passage. Radiation increased the rate of generation of stem-like spheres in both types of cells. The average survival fraction (SF2) of the cultured KYSE-150 compared with TE-1 stem-like spheres after 2 Gy of radiation was 0.81 ± 0.03 vs 0.87 ± 0.01 (P < 0.05), while the average SF2 of KYSE-150 compared with TE-1 parental cells was 0.69 ± 0.04 vs 0.80 ± 0.03, P < 0.05. In the esophageal parental cells, irradiation dose-dependently induced G2 arrest. Stem-like esophageal spheres were resistant to irradiation-induced G2 arrest without significant changes in the percentage population of irradiated stem-like cells. Under irradiation at 0, 4, and 8 Gy, the CD44+CD271+ cell percentage for KYSE150 parental cells was 1.08% ± 0.03% vs 1.29% ± 0.07% vs 1.11% ± 0.09%, respectively; the CD44+CD271+ cell percentage for TE1 parental cells was 1.16% ± 0.11% vs 0.97% ± 0.08% vs 1.45% ± 0.35%, respectively. The differences were not statistically significant. Under irradiation at 0, 4, and 8 Gy, the CD44+CD271+ cell percentage for KYSE-150 stem-like spheres was 35.83% ± 1.23% vs 44.9% ± 1.67% vs 57.77% ± 1.88%, respectively; the CD44+CD271+ cell percentage for TE1 stem-like spheres was 16.07% ± 0.91% vs 22.67% ± 1.12%, 16.07% ± 0.91% vs 33.27% ± 1.07%, respectively. The 4 and 8 Gy irradiated KYSE-150 and TE-1 stem-like spheres were compared with the 0 Gy irradiated group, and the differences were statistically significant (P < 0.05). CONCLUSION: The KYSE-150 and TE-1 stem-like spheres are more radioresistant than their parental cells which may suggest that cancer stem cells are related to radioresistance. PMID:25561796

  9. Radiobiological characteristics of cancer stem cells from esophageal cancer cell lines.

    PubMed

    Wang, Jian-Lin; Yu, Jing-Ping; Sun, Zhi-Qiang; Sun, Su-Ping

    2014-12-28

    To study the cancer stem cell population in esophageal cancer cell lines KYSE-150 and TE-1 and identify whether the resulting stem-like spheroid cells display cancer stem cells and radiation resistance characteristics. A serum-free medium (SFM) suspension was used to culture esophageal cancer stem cell lines and enrich the esophageal stem-like spheres. A reverse transcription polymerase chain reaction assay was used to detect stem cell gene expression in the spheroid cells. Radiosensitivity of stem-like spheres and parental cells were evaluated by clonogenic assays. Furthermore, different cells after different doses of irradiation were tested to evaluate the change in sphere formation, cell cycle and CD44(+)CD271(+) expression of tumor stem-like spheroid cells using flow cytometry before and after irradiation. The cells were observed to generate an increased number of spheres in SFM with increasing cell passage. Radiation increased the rate of generation of stem-like spheres in both types of cells. The average survival fraction (SF2) of the cultured KYSE-150 compared with TE-1 stem-like spheres after 2 Gy of radiation was 0.81 ± 0.03 vs 0.87 ± 0.01 (P < 0.05), while the average SF2 of KYSE-150 compared with TE-1 parental cells was 0.69 ± 0.04 vs 0.80 ± 0.03, P < 0.05. In the esophageal parental cells, irradiation dose-dependently induced G2 arrest. Stem-like esophageal spheres were resistant to irradiation-induced G2 arrest without significant changes in the percentage population of irradiated stem-like cells. Under irradiation at 0, 4, and 8 Gy, the CD44(+)CD271(+) cell percentage for KYSE150 parental cells was 1.08% ± 0.03% vs 1.29% ± 0.07% vs 1.11% ± 0.09%, respectively; the CD44(+)CD271(+) cell percentage for TE1 parental cells was 1.16% ± 0.11% vs 0.97% ± 0.08% vs 1.45% ± 0.35%, respectively. The differences were not statistically significant. Under irradiation at 0, 4, and 8 Gy, the CD44(+)CD271(+) cell percentage for KYSE-150 stem-like spheres was 35.83% ± 1.23% vs 44.9% ± 1.67% vs 57.77% ± 1.88%, respectively; the CD44(+)CD271(+) cell percentage for TE1 stem-like spheres was 16.07% ± 0.91% vs 22.67% ± 1.12%, 16.07% ± 0.91% vs 33.27% ± 1.07%, respectively. The 4 and 8 Gy irradiated KYSE-150 and TE-1 stem-like spheres were compared with the 0 Gy irradiated group, and the differences were statistically significant (P < 0.05). The KYSE-150 and TE-1 stem-like spheres are more radioresistant than their parental cells which may suggest that cancer stem cells are related to radioresistance.

  10. U.S. Postal Service Improvements Act of 2011

    THOMAS, 112th Congress

    Sen. Collins, Susan M. [R-ME

    2011-02-15

    Senate - 09/06/2011 Committee on Homeland Security and Governmental Affairs. Hearings held. Hearings printed: S.Hrg. 112-271. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  11. 77 FR 22806 - Proposed Extension of Existing Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-17

    ... annually. Total Burden Cost (capital/startup): $0. Total Burden Cost (operating/maintenance): $45,979... (Section 8(f) Payments) 1,425 ESA-100 (20 SFR 702.201) 840 LS-271 (Self Insurance Application) 60 LS-274...

  12. 7 CFR 273.3 - Residency.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... resident of a shelter for battered women and children as defined in § 271.2 and was a member of a household containing the person who had abused him or her. Residents of shelters for battered women and children shall...

  13. 7 CFR 273.3 - Residency.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... individual is a resident of a shelter for battered women and children as defined in § 271.2 and was a member of a household containing the person who had abused him or her. Residents of shelters for battered...

  14. 7 CFR 273.11 - Action on households with special circumstances.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... battered women and children. (1) Prior to certifying its residents under this paragraph, the State agency shall determine that the shelter for battered women and children meets the definition in § 271.2 and...

  15. 7 CFR 273.11 - Action on households with special circumstances.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... battered women and children. (1) Prior to certifying its residents under this paragraph, the State agency shall determine that the shelter for battered women and children meets the definition in § 271.2 and...

  16. Postal Operations Sustainment and Transformation Act of 2011

    THOMAS, 112th Congress

    Sen. Carper, Thomas R. [D-DE

    2011-05-17

    Senate - 09/06/2011 Committee on Homeland Security and Governmental Affairs. Hearings held. Hearings printed: S.Hrg. 112-271. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  17. Harmful Algal Blooms and Hypoxia Research and Control Amendments Act of 2009

    THOMAS, 111th Congress

    Sen. Snowe, Olympia J. [R-ME

    2009-05-01

    Senate - 02/04/2010 Placed on Senate Legislative Calendar under General Orders. Calendar No. 271. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  18. 34 CFR 271.20 - What conditions must an applicant meet to obtain funding?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., color, sex, age, or handicapping condition. (i) The applicant must have familiarity with materials used..., gender, age or handicapping condition. (l) The project must have an adequate budget to support the...

  19. 34 CFR 271.20 - What conditions must an applicant meet to obtain funding?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., color, sex, age, or handicapping condition. (i) The applicant must have familiarity with materials used..., gender, age or handicapping condition. (l) The project must have an adequate budget to support the...

  20. 78 FR 54240 - North Pacific Fishery Management Council; Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-03

    ... Plan Team (CPT) will meet in Seattle, WA. DATES: The meeting will be held September 17-20, 2013--from 9... Stram, at (907) 271-2809. SUPPLEMENTARY INFORMATION: The Plan Team meeting agenda includes review of...

  1. Performance Characterization of Loctite (Registered Trademark) 242 and 271 Liquid Locking Compounds (LLCs) as a Secondary Locking Feature for International Space Station (ISS) Fasteners

    NASA Technical Reports Server (NTRS)

    Dube, Michael J.; Gamwell, Wayne R.

    2011-01-01

    Several International Space Station (ISS) hardware components use Loctite (and other polymer based liquid locking compounds (LLCs)) as a means of meeting the secondary (redundant) locking feature requirement for fasteners. The primary locking method is the fastener preload, with the application of the Loctite compound which when cured is intended to resist preload reduction. The reliability of these compounds has been questioned due to a number of failures during ground testing. The ISS Program Manager requested the NASA Engineering and Safety Center (NESC) to characterize and quantify sensitivities of Loctite being used as a secondary locking feature. The findings and recommendations provided in this investigation apply to the anaerobic LLCs Loctite 242 and 271. No other anaerobic LLCs were evaluated for this investigation. This document contains the findings and recommendations of the NESC investigation

  2. Plant Endocytosis Requires the ER Membrane-Anchored Proteins VAP27-1 and VAP27-3.

    PubMed

    Stefano, Giovanni; Renna, Luciana; Wormsbaecher, Clarissa; Gamble, Jessie; Zienkiewicz, Krzysztof; Brandizzi, Federica

    2018-05-22

    Through yet-undefined mechanisms, the plant endoplasmic reticulum (ER) has a critical role in endocytosis. The plant ER establishes a close association with endosomes and contacts the plasma membrane (PM) at ER-PM contact sites (EPCSs) demarcated by the ER membrane-associated VAMP-associated-proteins (VAP). Here, we investigated two plant VAPs, VAP27-1 and VAP27-3, and found an interaction with clathrin and a requirement for the homeostasis of clathrin dynamics at endocytic membranes and endocytosis. We also demonstrated direct interaction of VAP27-proteins with phosphatidylinositol-phosphate lipids (PIPs) that populate endocytic membranes. These results support that, through interaction with PIPs, VAP27-proteins bridge the ER with endocytic membranes and maintain endocytic traffic, likely through their interaction with clathrin. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

  3. 21 CFR 1403.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... OFFICE OF NATIONAL DRUG CONTROL POLICY UNIFORM ADMINISTRATIVE REQUIREMENTS FOR GRANTS AND COOPERATIVE... Status Report” (or other equivalent report); (2) For construction grants, the SF-271 “Outlay Report and Request for Reimbursement” (or other equivalent report). Federally recognized Indian tribal government...

  4. 34 CFR 271.20 - What conditions must an applicant meet to obtain funding?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... a plan of operation for the proposed project that includes: (1) An effective plan of management that..., gender, age or handicapping condition. (l) The project must have an adequate budget to support the...

  5. 34 CFR 271.20 - What conditions must an applicant meet to obtain funding?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... a plan of operation for the proposed project that includes: (1) An effective plan of management that..., gender, age or handicapping condition. (l) The project must have an adequate budget to support the...

  6. 34 CFR 271.20 - What conditions must an applicant meet to obtain funding?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... a plan of operation for the proposed project that includes: (1) An effective plan of management that..., gender, age or handicapping condition. (l) The project must have an adequate budget to support the...

  7. 75 FR 57849 - Maintenance of Incombustible Content of Rock Dust in Underground Coal Mines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-23

    ... Richard Arrington Jr. Blvd N, Birmingham, AL 35203. November 16, 2010 Hilton Suites (859) 271-4000... Fairness Act (SBREFA) Pursuant to the Regulatory Flexibility Act (RFA) of 1980, as amended by SBREFA, MSHA...

  8. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under the... states the conditions under which spectrum is made available and licensed for the provision of wireless...

  9. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and...-stop shop for agency alternative fuel and vehicle information needs. http://www.afdc.nrel.gov. (5) The...

  10. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and...-stop shop for agency alternative fuel and vehicle information needs. http://www.afdc.nrel.gov. (5) The...

  11. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and...-stop shop for agency alternative fuel and vehicle information needs. http://www.afdc.nrel.gov. (5) The...

  12. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and...-stop shop for agency alternative fuel and vehicle information needs. http://www.afdc.nrel.gov. (5) The...

  13. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... organically produced products or ingredients with the substance used. (e) The handler of an organic handling... MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic Production and Handling...

  14. 75 FR 2489 - North Pacific Fishery Management Council; Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-15

    ...) Implementation Team will have a teleconference on February 4, 2010. The conference number to call is (907) 271... reviewed on the Council website at http://www.alaskafisheries.noaa.gov/npfmc/ and rank the priority of all...

  15. 75 FR 13297 - Alaska Native Claims Selection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... for 118.47 acres, located southeast of the Native village of Hughes, Alaska. Notice of the decision...: The Bureau of Land Management by phone at 907-271-5960, or by e-mail at ak[email protected]ak.blm.gov...

  16. A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination.

    PubMed

    Ribeiro, Lucas Ferreira; Tullman, Jennifer; Nicholes, Nathan; Silva, Sérgio Ruschi Bergamachi; Vieira, Davi Serradella; Ostermeier, Marc; Ward, Richard John

    2016-01-01

    Saccharification of lignocellulosic material by xylanases and other glycoside hydrolases is generally conducted at high concentrations of the final reaction products, which frequently inhibit the enzymes used in the saccharification process. Using a random nonhomologous recombination strategy, we have fused the GH11 xylanase from Bacillus subtilis (XynA) with the xylose binding protein from Escherichia coli (XBP) to produce an enzyme that is allosterically stimulated by xylose. The pT7T3GFP_XBP plasmid containing the XBP coding sequence was randomly linearized with DNase I, and ligated with the XynA coding sequence to create a random XynA-XBP insertion library, which was used to transform E. coli strain JW3538-1 lacking the XBP gene. Screening for active XBP was based on the expression of GFP from the pT7T3GFP_XBP plasmid under the control of a xylose inducible promoter. In the presence of xylose, cells harboring a functional XBP domain in the fusion protein (XBP+) showed increased GFP fluorescence and were selected using FACS. The XBP+ cells were further screened for xylanase activity by halo formation around xylanase producing colonies (XynA+) on LB-agar-xylan media after staining with Congo red. The xylanase activity ratio with xylose/without xylose in supernatants from the XBP+/XynA+ clones was measured against remazol brilliant blue xylan. A clone showing an activity ratio higher than 1.3 was selected where the XynA was inserted after the asparagine 271 in the XBP, and this chimera was denominated as XynA-XBP271. The XynA-XBP271 was more stable than XynA at 55 °C, and in the presence of xylose the catalytic efficiency was ~3-fold greater than the parental xylanase. Molecular dynamics simulations predicted the formation of an extended protein-protein interface with coupled movements between the XynA and XBP domains. In the XynA-XBP271 with xylose bound to the XBP domain, the mobility of a β-loop in the XynA domain results in an increased access to the active site, and may explain the observed allosteric activation. The approach presented here provides an important advance for the engineering enzymes that are stimulated by the final product.

  17. cAMP and in vivo hypoxia induce tob, ifr1, and fos expression in erythroid cells of the chick embryo.

    PubMed

    Dragon, Stefanie; Offenhäuser, Nina; Baumann, Rosemarie

    2002-04-01

    During avian embryonic development, terminal erythroid differentiation occurs in the circulation. Some of the key events, such as the induction of erythroid 2,3-bisphosphoglycerate (2,3-BPG), carbonic anhydrase (CAII), and pyrimidine 5'-nucleotidase (P5N) synthesis are oxygen dependent (Baumann R, Haller EA, Schöning U, and Weber M, Dev Biol 116: 548-551, 1986; Dragon S and Baumann R, Am J Physiol Regulatory Integrative Comp Physiol 280: R870-R878, 2001; Dragon S, Carey C, Martin K, and Baumann R, J Exp Biol 202: 2787-2795, 1999; Dragon S, Glombitza S, Götz R, and Baumann R, Am J Physiol Regulatory Integrative Comp Physiol 271: R982-R989, 1996; Dragon S, Hille R, Götz R, and Baumann R, Blood 91: 3052-3058, 1998; Million D, Zillner P, and Baumann R, Am J Physiol Regulatory Integrative Comp Physiol 261: R1188-R1196, 1991) in an indirect way: hypoxia stimulates the release of norepinephrine (NE)/adenosine into the circulation (Dragon et al., J Exp Biol 202: 2787-2795, 1999; Dragon et al., Am J Physiol Regulatory Integrative Comp Physiol 271: R982-R989, 1996). This leads via erythroid beta-adrenergic/adenosine A(2) receptor activation to a cAMP signal inducing several proteins in a transcription-dependent manner (Dragon et al., Am J Physiol Regulatory Integrative Comp Physiol 271: R982-R989, 1996; Dragon et al., Blood 91: 3052-3058, 1998; Glombitza S, Dragon S, Berghammer M, Pannermayr M, and Baumann R, Am J Physiol Regulatory Integrative Comp Physiol 271: R973-R981, 1996). To understand how the cAMP-dependent processes are initiated, we screened an erythroid cDNA library for cAMP-regulated genes. We detected three genes that were strongly upregulated (>5-fold) by cAMP in definitive and primitive red blood cells. They are homologous to the mammalian Tob, Ifr1, and Fos proteins. In addition, the genes are induced in the intact embryo during short-term hypoxia. Because the genes are regulators of proliferation and differentiation in other cell types, we suggest that cAMP might promote general differentiating processes in erythroid cells, thereby allowing adaptive modulation of the latest steps of erythroid differentiation during developmental hypoxia.

  18. Dietary protein during gestation affects maternal insulin-like growth factor, insulin-like growth factor binding protein, leptin concentrations, and fetal growth in heifers.

    PubMed

    Sullivan, T M; Micke, G C; Perkins, N; Martin, G B; Wallace, C R; Gatford, K L; Owens, J A; Perry, V E A

    2009-10-01

    The influence of supplemental protein during gestation on maternal hormones and fetal growth was determined in composite beef heifers. At AI, 118 heifers were stratified by BW within each composite genotype (BeefX = 1/2 Senepol, 1/4 Brahman, 1/8 Charolais, 1/8 Red Angus and CBX = 1/2 Senepol, 1/4 Brahman, 1/4 Charolais) into 4 treatment groups: high high (HH = 1.4 kg CP/d for first and second trimesters of gestation), high low (HL = 1.4 kg of CP/d for first trimester and 0.4 kg of CP/d for second trimester), low high (lowH = 0.4 kg CP/d for first trimester and 1.4 kg of CP/d and for second trimester), or low low (LL = 0.4 kg CP/d for first and second trimesters). Maternal plasma IGF-I and -II, total IGFBP, and leptin concentrations were determined at 14 d before AI and at d 28, 82, 179, and 271 post-AI (mean gestation length 286 d), and leptin concentrations were also determined at calving. Increased dietary protein increased maternal plasma IGF-I (P < 0.001 on d 28, 82, and 179), IGF-II (P = 0.01 on d 82; P = 0.04 on d 271), and total IGFBP (P = 0.002 on d 82; P = 0.005 on d 179; P = 0.03 on d 271). Maternal plasma IGF-I at d 271 was negatively associated with calf crown-rump length at birth (P = 0.003). BeefX had greater birth weight calves (P = 0.01), greater IGF-II (P < 0.001), increased ratios of IGF-I:total IGFBP (P = 0.008) and IGF-II:total IGFBP (P < 0.001), and reduced total IGFBP compared with CBX (P = 0.02). Increased dietary protein during second trimester increased maternal plasma leptin at calving (P = 0.005). Maternal plasma leptin near term was positively associated with heifer BCS (P = 0.02) and with calf birth weight (P = 0.04), and at calving was positively associated with heifer age at AI (P = 0.02). These findings suggest that maternal dietary protein, age, and genotype influence plasma concentrations of metabolic hormones and fetal growth in Bos indicus-influenced heifers.

  19. 78 FR 47048 - Notice of Availability of Final Environmental Impact Statement (Final EIS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-02

    ... may be viewed online or during regular business hours at the following locations: 1. Online at www..., Anchorage, AK 99513. Ms. Grey may be contacted during business hours at (907) 271-5453 (telephone) and (907...

  20. 29 CFR 97.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... limited to: (1) Final performance or progress report. (2) Financial Status Report (SF 269) or Outlay Report and Request for Reimbursement for Construction Programs (SF-271) (as applicable.) (3) Final request for payment (SF-270) (if applicable). (4) Invention disclosure (if applicable). (5) Federally...

  1. 36 CFR 1207.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... limited to: (1) Final performance or progress report. (2) Financial Status Report (SF 269) or Outlay Report and Request for Reimbursement for Construction Programs (SF-271) (as applicable). (3) Final request for payment (SF-270) (if applicable). (4) Invention disclosure (if applicable). (5) Federally...

  2. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  3. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  4. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  5. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  6. 40 CFR 60.271 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... (c) Control device means the air pollution control equipment used to remove particulate matter... air pollution control device. (e) Charge means the addition of iron and steel scrap or other materials... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF...

  7. Publications - GMC 271 | Alaska Division of Geological & Geophysical

    Science.gov Websites

    ') and of core (9,683-9,694') from the ARCO Alaska Inc. Tulaga #1 well Authors: Unknown Publication Date Reference Unknown, 1996, Vitrinite reflectance maceral data of cuttings (1,200-11,742') and of core (9,683

  8. 41 CFR 101-27.101 - General.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 27-INVENTORY MANAGEMENT 27.1-Stock... inventory which is that portion carried to satisfy average expected demand, and safety stock which is that portion carried for protection against stock depletion occurring when demand exceeds average expected...

  9. Effects of elevated CO2 and ozone on phenolic glycosides of trembling aspen

    Treesearch

    James K. Nitao; Muraleedharan G. Nair; William J. Mattson; Daniel A. Herms; Bruce A. Birr; Mark D. Coleman; Terry M. Trier; J. G. Isebrands

    1996-01-01

    We tested the effects of elevated CO2 and ozone on concentrations of the phenolic glycosides salicortin and tremulacin in immature and mature foliage of the trembling aspen (Populus tremuloides) clones 216, 259, and 271.

  10. 48 CFR 1371.107 - Performance.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Performance. 1371.107 Section 1371.107 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE DEPARTMENT SUPPLEMENTAL... Performance. Insert clause 1352.271-76, Performance, in all solicitations and contracts for ship construction...

  11. 48 CFR 1371.107 - Performance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Performance. 1371.107 Section 1371.107 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE DEPARTMENT SUPPLEMENTAL... Performance. Insert clause 1352.271-76, Performance, in all solicitations and contracts for ship construction...

  12. 48 CFR 1371.107 - Performance.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Performance. 1371.107 Section 1371.107 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE DEPARTMENT SUPPLEMENTAL... Performance. Insert clause 1352.271-76, Performance, in all solicitations and contracts for ship construction...

  13. 48 CFR 1371.107 - Performance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Performance. 1371.107 Section 1371.107 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE DEPARTMENT SUPPLEMENTAL... Performance. Insert clause 1352.271-76, Performance, in all solicitations and contracts for ship construction...

  14. 48 CFR 1371.107 - Performance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Performance. 1371.107 Section 1371.107 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE DEPARTMENT SUPPLEMENTAL... Performance. Insert clause 1352.271-76, Performance, in all solicitations and contracts for ship construction...

  15. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... marketers which are equivalent to those under subpart H of part 279 of this chapter; and (g) Standards for...) and (b) Sec. 279.66(b) Sec. 279.72(b) 1 Contains additional new definitions that were not included in...

  16. 47 CFR 80.271 - Technical requirements for portable survival craft radiotelephone transceivers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...:9 with no significant adverse effect upon the performance of the device; (5) The transceivers must... Commission's database. [51 FR 31213, Sept. 2, 1986, as amended at 63 FR 36607, July 7, 1998; 73 FR 4483, Jan...

  17. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  18. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  19. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  20. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  1. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... and 266. These standards shall include: (a) Technical standards for tanks, containers, waste piles...-closure monitoring and maintenance; (e) Groundwater monitoring; (f) Security to prevent unauthorized access to the facility; (g) Facility personnel training; (h) Inspections, monitoring, recordkeeping, and...

  2. Pilot tracking performance during successive in-flight simulated instrument approaches.

    DOT National Transportation Integrated Search

    1972-02-01

    Eight instrument rated pilots with flying experience ranging from 600 to 12,271 hours each flew 10 simulated ILS instrument approaches in a single engine, general aviation aircraft equipped with a primary flight display arranged in a conventional 'T'...

  3. 75 FR 69457 - Alaska Native Claims Selection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-12

    ..., AA-8102-30, AA-8102-31, AA-8102-32, AA-8102-33, AA-8102-34, AA-8102-47; LLAK965000-L14100000-KC0000-P... at 907-271-5960, by e-mail at ak[email protected] , or by telecommunication device (TTD...

  4. 76 FR 65530 - Alaska Native Claims Selection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-21

    ... 26; Secs. 33, 35, and 36. Containing 3,689 acres. T. 21 N., R. 47 W., Sec. 34. Containing 457.50.... FOR FURTHER INFORMATION CONTACT: The BLM by phone at 907-271-5960 or by e-mail at ak.blm.conveyance...

  5. 76 FR 75899 - Alaska Native Claims Selection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    ...., Tracts C, D and E; Sec. 36. Containing approximately 2,327 acres. T. 47 S., R. 60 W., Sec. 4, lots 1 to 4... phone at (907) 271-5960 or by email at ak[email protected] . Persons who use a Telecommunications...

  6. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., and 805-806 MHz. (3) 698-746 MHz. (4) 1390-1392 MHz. (5) 1392-1395 MHz and 1432-1435 MHz. (6) 1670-1675 MHz. (7) [Reserved] (8) 1710-1755 MHz and 2110-2155 MHz. (9) 2495-2690 MHz. (c) Scope. The rules...

  7. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  8. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  9. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  10. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  11. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for... environment. Note: This paragraph requires that States have a mechanism (e.g., an administrative cease and... obtainable against any person who knowingly transports any hazardous waste to an unpermitted facility; who...

  12. 7 CFR 3016.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... are not limited to: (1) Final performance or progress report. (2) Financial Status Report (SF 269) or Outlay Report and Request for Reimbursement for Construction Programs (SF-271) (as applicable.) (3) Final request for payment (SF-270) (if applicable). (4) Invention disclosure (if applicable). (5) Federally...

  13. 40 CFR 31.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., Federal agencies may extend this timeframe. These may include but are not limited to: (1) Final... Reimbursement for Construction Programs (SF-271) (as applicable.) (3) Final request for payment (SF-270) (if applicable). (4) Invention disclosure (if applicable). (5) Federally-owned property report: In accordance...

  14. 45 CFR 2541.500 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., Federal agencies may extend this time frame. These may include but are not limited to: (1) Final... Reimbursement for Construction Programs (SF-271) (as applicable); (3) Final request for payment (SF-270) (if applicable); (4) Invention disclosure (if applicable); (5) Federally-owned property report. In accordance...

  15. 38 CFR 43.50 - Closeout.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., Federal agencies may extend this timeframe. These may include but are not limited to: (1) Final... Reimbursement for Construction Programs (SF-271) (as applicable.) (3) Final request for payment (SF-270) (if applicable). (4) Invention disclosure (if applicable). (5) Federally-owned property report: In accordance...

  16. 40 CFR 124.5 - Modification, revocation and reissuance, or termination of permits.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... §§ 123.25 (NPDES), 145.11 (UIC), 233.26 (404), and 271.14 (RCRA).) Permits (other than PSD permits) may... requirements of § 124.3. (g)(1) (Reserved for PSD Modification Provisions). (2) PSD permits may be terminated...

  17. 40 CFR 124.5 - Modification, revocation and reissuance, or termination of permits.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... §§ 123.25 (NPDES), 145.11 (UIC), 233.26 (404), and 271.14 (RCRA).) Permits (other than PSD permits) may... requirements of § 124.3. (g)(1) (Reserved for PSD Modification Provisions). (2) PSD permits may be terminated...

  18. 40 CFR 124.5 - Modification, revocation and reissuance, or termination of permits.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... §§ 123.25 (NPDES), 145.11 (UIC), 233.26 (404), and 271.14 (RCRA).) Permits (other than PSD permits) may... requirements of § 124.3. (g)(1) (Reserved for PSD Modification Provisions). (2) PSD permits may be terminated...

  19. 40 CFR 124.5 - Modification, revocation and reissuance, or termination of permits.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... §§ 123.25 (NPDES), 145.11 (UIC), 233.26 (404), and 271.14 (RCRA).) Permits (other than PSD permits) may... requirements of § 124.3. (g)(1) (Reserved for PSD Modification Provisions). (2) PSD permits may be terminated...

  20. 40 CFR 124.5 - Modification, revocation and reissuance, or termination of permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... §§ 123.25 (NPDES), 145.11 (UIC), 233.26 (404), and 271.14 (RCRA).) Permits (other than PSD permits) may... requirements of § 124.3. (g)(1) (Reserved for PSD Modification Provisions). (2) PSD permits may be terminated...

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